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Sample records for synophyrs curly eyelashes

  1. Eyelash Transplantation Using Leg Hair by Follicular Unit Extraction

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    Sanusi Umar, MD

    2015-03-01

    Full Text Available Summary: Fine hairs of the head and nape areas have been used as donor sources in eyelash transplantation but are straight, coarse, and grow rapidly, requiring frequent eyelash maintenance. This is the first reported case of eyelash transplantation by follicular unit extraction using leg hair as a donor source; findings were compared with that of another patient who underwent a similar procedure with donor hairs from the nape area. Although both patients reported marked improvement in fullness of eyelashes within 3 months postsurgery, the transplanted leg hair eyelashes required less frequent trimming (every 5–6 weeks compared with nape hair eyelashes (every 2–3 weeks. Additionally, in leg hair eyelashes, the need for perming to sustain a natural looking eyelash curl was eliminated. Eyelash transplantation using leg donor hair in hirsute women may result in good cosmetic outcomes and require less maintenance compared with nape donor hair.

  2. Iris Heterochromia and Unilateral Eyelash Hypertrichosis

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    Rao, Rajesh C.; Ballard, Tiffany N.S.; Chen, Teresa C.

    2015-01-01

    An 87-year-old female with a history of open-angle glaucoma presented with longer, thicker eyelashes on the right side compared to the left. Her irides were also different colors, as the right iris was brown and the left was hazel (green-brown). The patient had noticed a gradual darkening of her right iris and lengthening of her eyelashes over the last year, but denied visual changes or foreign-body sensation. Past medical history included a remote history of breast cancer treated by lumpecto...

  3. [Trichotillomania involving the eyelashes: about a case].

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    Touzani, Khadija Drissi; Lamari, Zineb; Chraibi, Fouad; Abdellaoui, Meryem; Andaloussi, Idriss Benatiya

    2017-01-01

    Trichotillomania is a neglected psychiatric disorder characterized by the urge to pull out the hair, the eyebrows or any other hair, but rarely the eyelashes. In the Diagnostic and Statistical Manual of Mental Disorders it is defined as habits and pulses disturbance. We here report the case of a girl with trichotillomania involving her left eyelashes due to low self-esteem. The disease may be episodic but it is usually chronic and difficult to treat. Given the possible clinical and psychiatric complications, early and accurate diagnosis is essential. Cognitive-behavioral therapy is the gold standard treatment.

  4. Ocular discomforts following eyelash extension | Koffuor | Journal of ...

    African Journals Online (AJOL)

    Eyelash extension has become common practice for enhancing beauty among Ghanaian women on occasions such as weddings, festivities, and other social gatherings including funerals. This study was therefore conducted to ascertain the effect of eyelash extension on the eyelid and on vision. One hundred and twenty ...

  5. Bimatoprost for the treatment of eyelash, eyebrow and scalp alopecia.

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    Barrón-Hernández, Yevher Lorena; Tosti, Antonella

    2017-04-01

    Alopecia is a common condition observed among people of all ages. It is a disorder that can involve only the scalp as observed in androgenetic alopecia or scalp and body as in alopecia areata or patients under chemotherapy treatment. There are several treatment options with different safety and efficacy outcomes. Bimatoprost, a synthetic prostamide F2α analog originally approved for the treatment of ocular hypertension and open-angle glaucoma, is now FDA approved as a 0.03%, solution to be applied once daily to increase eyelashes growth. Areas covered: In this review, the authors evaluate the role of bimatoprost in idiopathic hypotrichosis of the eyelashes, in hypotrichosis of the eyelashes associated to chemotherapy, in alopecia areata of the eyelashes and eyebrows and in androgenetic alopecia. In addition, pharmacokinetics, pharmacodynamics, safety and tolerability of bimatoprost are discussed. Expert opinion: Bimatoprost will likely be the third FDA approved weapon in the fight against hair loss. Prostaglandin analogs are the only possible treatment for hypotrichosis and alopecia of the eyelashes regardless of its etiology. Eyebrow hypotrichosis due to alopecia areata or frontal fibrosis alopecia can also possibly benefit of these medications.

  6. Eyelash Extension Use among Female Students in a Tertiary ...

    African Journals Online (AJOL)

    Others were curiosity, peer pressure, and replacement therapy. The most common complication was itching 45.8% (n = 142). Others were redness, pain, heavy eyelids, loss of lashes, casting of shadow in vision, tearing, burning sensation, foreign body sensation, and boils (stye) on the eyelid. Conclusion: Eyelash extension ...

  7. Heterochromia of the Hair and Eyelashes with Blaschkoid Dyspigmentation.

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    Dumitrascu, Catalina I; Hoss, Elika; Hogeling, Marcia

    2016-01-01

    Heterochromia of the hair involves the presence of two distinctive colors of scalp hair in one individual. We report the case of a 4-year-old girl with reddish streaks of scalp hair, heterochromia of the eyelashes, and cutaneous hypopigmentation following the lines of Blaschko. © 2016 Wiley Periodicals, Inc.

  8. An evaluation of the safety and efficacy of bimatoprost for eyelash growth in pediatric subjects

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    Borchert M

    2016-03-01

    Full Text Available Mark Borchert,1 Suzanne Bruce,2 David Wirta,3 Steven G Yoelin,4 Sungwook Lee,5 Cheri Mao,5 Amanda VanDenburgh5 1Children’s Hospital Los Angeles, Los Angeles, CA, USA; 2Suzanne Bruce and Associates, PA, Houston, TX, USA; 3David Wirta and Associates, Newport Beach, CA, USA; 4Medical Associates Inc., Newport Beach, CA, USA; 5Allergan plc, Irvine, CA, USA Purpose: Evaluate the safety and effectiveness of bimatoprost 0.03% for treatment of eyelash hypotrichosis in a pediatric population. Patients and methods: This multicenter, randomized, double-masked, parallel-group study was conducted at seven sites in the US and Brazil. Subjects with eyelash hypotrichosis caused by chemotherapy or alopecia areata (aged 5–17 years or healthy adolescents aged 15–17 years were enrolled (N=71. Subjects applied bimatoprost 0.03% or vehicle to upper eyelid margins once nightly for 4 months and were followed for 1 month post-treatment. Eyelash prominence was assessed using the validated 4-grade Global Eyelash Assessment scale with photonumeric guide. Changes in eyelash length, thickness, and darkness were measured by digital image analysis. Safety was assessed by adverse events and ophthalmic observations. Results: Eyelash prominence improved in a significantly greater proportion of subjects treated with bimatoprost compared with vehicle at month 4 (70.8% versus 26.1%; P<0.001. This benefit was sustained at month 5 post-treatment assessment. Digital image analysis measures were significantly improved with bimatoprost. Significant treatment benefits with bimatoprost versus vehicle were evident among the healthy adolescents but not in the postchemotherapy or alopecia areata subgroups. The safety profile of bimatoprost was consistent with previous studies in adults. Conclusion: Bimatoprost was safe and well tolerated in pediatric subjects with eyelash hypotrichosis. In this study with limited sample size, subgroup analyses showed that treatment was effective in

  9. Congo Red Interactions with Curli-Producing E. coli and Native Curli Amyloid Fibers.

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    Reichhardt, Courtney; Jacobson, Amy N; Maher, Marie C; Uang, Jeremy; McCrate, Oscar A; Eckart, Michael; Cegelski, Lynette

    2015-01-01

    Microorganisms produce functional amyloids that can be examined and manipulated in vivo and in vitro. Escherichia coli assemble extracellular adhesive amyloid fibers termed curli that mediate adhesion and promote biofilm formation. We have characterized the dye binding properties of the hallmark amyloid dye, Congo red, with curliated E. coli and with isolated curli fibers. Congo red binds to curliated whole cells, does not inhibit growth, and can be used to comparatively quantify whole-cell curliation. Using Surface Plasmon Resonance, we measured the binding and dissociation kinetics of Congo red to curli. Furthermore, we determined that the binding of Congo red to curli is pH-dependent and that histidine residues in the CsgA protein do not influence Congo red binding. Our results on E. coli strain MC4100, the most commonly employed strain for studies of E. coli amyloid biogenesis, provide a starting point from which to compare the influence of Congo red binding in other E. coli strains and amyloid-producing organisms.

  10. Congo Red Interactions with Curli-Producing E. coli and Native Curli Amyloid Fibers.

    Directory of Open Access Journals (Sweden)

    Courtney Reichhardt

    Full Text Available Microorganisms produce functional amyloids that can be examined and manipulated in vivo and in vitro. Escherichia coli assemble extracellular adhesive amyloid fibers termed curli that mediate adhesion and promote biofilm formation. We have characterized the dye binding properties of the hallmark amyloid dye, Congo red, with curliated E. coli and with isolated curli fibers. Congo red binds to curliated whole cells, does not inhibit growth, and can be used to comparatively quantify whole-cell curliation. Using Surface Plasmon Resonance, we measured the binding and dissociation kinetics of Congo red to curli. Furthermore, we determined that the binding of Congo red to curli is pH-dependent and that histidine residues in the CsgA protein do not influence Congo red binding. Our results on E. coli strain MC4100, the most commonly employed strain for studies of E. coli amyloid biogenesis, provide a starting point from which to compare the influence of Congo red binding in other E. coli strains and amyloid-producing organisms.

  11. Low incidence of iris pigmentation and eyelash changes in 2 randomized clinical trials with unoprostone isopropyl 0.15%.

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    McCarey, Bernard E; Kapik, Barry M; Kane, Frances E

    2004-08-01

    To assess whether iris color and eyelash changes occur with the use of unoprostone for 2 years. The 2 clinical trials described herein were prospective, randomized, double-masked, active-controlled, parallel group, multicenter studies. A total of 1131 patients with primary open-angle glaucoma or ocular hypertension participated in 2 clinical trials and received either unoprostone isopropyl 0.15% (659), timolol maleate 0.5% (331), or betaxolol hydrochloride 0.5% (141), 1 drop per eye twice daily for up to 24 months. Color photographs (1:1 magnification) were taken of the iris and eyelid of each patient at baseline and at regular intervals thereafter through month 24 using a standardized camera system. Photography included 7 views of each eye plus a calibration photograph and a patient identification photograph, for a total of 16 photographs per patient per visit. Two independent (masked) readers subjectively compared baseline iris colors to subsequent visits. Side view photographs of the upper and lower eyelashes were used for the eyelash length analysis, with each having sufficient depth of field and a sufficient number of eyelashes in focus. Similarly, frontal eyelash views were used for the eyelash density analysis. Changes from baseline in iris color and eyelash length and density within and between treatment groups. Seven cases of iris color change (1.06%) were confirmed in patients treated with unoprostone for up to 24 months; no confirmed cases were reported in the timolol or betaxolol groups. In the unoprostone group, cases of iris color change were confirmed at months 12 (1 case), 18 (2 cases), and 24 (4 cases). No clinically relevant differences were observed among treatment groups for changes from baseline in eyelash length or density. Although iris hyperpigmentation and abnormal eyelash changes may occur after treatment with unoprostone, the incidence of these events appears to be low in the 2-year clinical study.

  12. Ocular argyrosis after long-term self-application of eyelash tint.

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    Gallardo, Mark J; Randleman, J Bradley; Price, Kristina M; Johnson, Daniel A; Acosta, Sharon; Grossniklaus, Hans E; Stulting, R Doyle

    2006-01-01

    To report cases of ocular argyrosis that developed after long-term self-application of commercially available eyelash tint. Observational case series. Case review, clinicopathologic analysis, and literature review. Three patients developed ocular argyrosis after the long-term self-application of Revlon Professional Roux Lash and Brow Tint (Colomer USA Corp, New York, New York, USA). Clinical evaluation revealed various degrees of silver deposition on the upper eyelid, lid margin, caruncle and conjunctiva, and diffuse Descemet's membrane deposits. In one case, histologic examination demonstrated silver deposition in the basement membrane and superficial substantia propria of the conjunctiva. Argyrosis can occur after long-term application of readily available eyelash tints, and the deposition of silver may be permanent. In certain circumstances, conjunctival argyrosis may simulate benign and malignant lesions, including conjunctival melanoma. These products should only be applied by trained cosmetologists.

  13. Safety and efficacy of bimatoprost solution 0.03% topical application in patients with chemotherapy-induced eyelash loss.

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    Ahluwalia, Gurpreet S

    2013-12-01

    Few dermatologic conditions carry as much anxiety and emotional distress as hair loss resulting from a disease condition such as alopecia areata or as a result of cytotoxic drug treatment, e.g., after chemotherapy. Bimatoprost 0.03% solution is a Food and Drug Administration-approved prescription product indicated for the treatment of eyelash hypotrichosis. The product was investigated in a double-masked, randomized, and placebo-controlled study in patients who had significant eyelash loss or hypotrichosis as a result of chemotherapy. Once-daily treatment with bimatoprost ophthalmic solution 0.03% to the upper eyelid margin restored eyelash growth and prominence more quickly than the slower, natural course of recovery observed in the vehicle control subjects. The eyelash prominence measured using a validated Global Eyelash Assessment (GEA) scale demonstrated a statistically significant increase over placebo following 6 months of treatment. Efficacy was also demonstrated using a validated objective digital image analysis methodology to show significant increase in eyelash length, thickness/fullness, and darkness in these patients. Bimatoprost was found to be well tolerated over the 1-year treatment period.

  14. Prevalence of Demodex mites in eyelashes among people of Oaxaca, Mexico.

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    Vargas-Arzola, Jaime; Reyes-Velasco, Leobardo; Segura-Salvador, Aristeo; Márquez-Navarro, Adrián; Díaz-Chiguer, Dylan L; Nogueda-Torres, Benjamín

    2012-06-01

    This study was carried out to determine the prevalence of Demodex mites in eyelashes of people from different places from Oaxaca. One thousand and ten patients underwent epilation of eight eyelashes from each eye and they were processed to search Demodex which was found in 208 (20%) of the patients and among them male was more affected than female, 119 (57%) and 89 (43%), respectively. There was not found a relationship between clinical manifestations among these patients since 101 (49%) and 107 (51%) of them were symptomatic and asymptomatic, respectively. The range of age that was more affected was 96-105 (100%), followed by 86-95 (75%) and 76-85 (64%) years old. The less affected were individuals of 15-25 years old (11%). Demodex folliculorum was relatively high in the eyelashes of individuals in young and old people in the rural region studied. These people are generally the most vulnerable patients because they live in overcrowded conditions and poor hygiene which facilitates the direct infection. Demodex brevis was found in only one sample.

  15. Curli fimbria: an Escherichia coli adhesin associated with human cystitis.

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    Cordeiro, Melina Aparecida; Werle, Catierine Hirsch; Milanez, Guilherme Paier; Yano, Tomomasa

    2016-01-01

    Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its mutant (ΔcsgA) were analyzed for their adhesion ability over HTB-9 (human bladder carcinoma), Vero (kidney cells of African green monkey) and HUVEC (human umbilical vein) cells in the presence of α-d-mannose. All the wild-type UPEC strains tested (100%) were able to adhere to all three cell types, while the UPEC-4 ΔcsgA mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells. The results suggest that curli fimbria has an important role in the adhesion processes associated with human UPEC-induced cystitis. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

  16. Opname van tevredenheid van B.Cur. - finalejaarstudente aan die ...

    African Journals Online (AJOL)

    The fourth-year students of 2001 were the first group to complete their studies with the revised curriculum. Thus it was important to evaluate student satisfaction. In order to achieve the purpose of the study, a quantitative survey design was used. The total population (N = 18) of the B Cur fourth-year group participated in the ...

  17. Effect of curtovirus species competitiveness in host plants on transmission and incidence of Beet severe curly top virus and Beet mild curly top virus

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    Curly top disease, caused by viruses in the genus Curtovirus, causes significant economic losses for sugarbeet and other crops throughout the western United States. Recent studies demonstrated the two most abundant curtovirus species in the US are Beet severe curly top virus (BSCTV) and Beet mild c...

  18. The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coli

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    Smith, Daniel R.; Price, Janet E.; Burby, Peter E.; Blanco, Luz P.; Chamberlain, Justin; Chapman, Matthew R.

    2017-01-01

    Curli amyloid fibers are the major protein component of the extracellular matrix produced by Enterobacteriaceae during biofilm formation. Curli are required for proper biofilm development and environmental persistence by Escherichia coli. Here, we present a complete and vetted genetic analysis of functional amyloid fiber biogenesis. The Keio collection of single gene deletions was screened on Congo red indicator plates to identify E. coli mutants that had defective amyloid production. We discovered that more than three hundred gene products modulated curli production. These genes were involved in fundamental cellular processes such as regulation, environmental sensing, respiration, metabolism, cell envelope biogenesis, transport, and protein turnover. The alternative sigma factors, σS and σE, had opposing roles in curli production. Mutations that induced the σE or Cpx stress response systems had reduced curli production, while mutant strains with increased σS levels had increased curli production. Mutations in metabolic pathways, including gluconeogenesis and the biosynthesis of lipopolysaccharide (LPS), produced less curli. Regulation of the master biofilm regulator, CsgD, was diverse, and the screen revealed several proteins and small RNAs (sRNA) that regulate csgD messenger RNA (mRNA) levels. Using previously published studies, we found minimal overlap between the genes affecting curli biogenesis and genes known to impact swimming or swarming motility, underlying the distinction between motile and sessile lifestyles. Collectively, the diversity and number of elements required suggest curli production is part of a highly regulated and complex developmental pathway in E. coli. PMID:29088115

  19. Patient-reported outcomes of bimatoprost for eyelash growth: results from a randomized, double-masked, vehicle-controlled, parallel-group study.

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    Fagien, Steven; Walt, John G; Carruthers, Jean; Cox, Sue Ellen; Wirta, David; Weng, Emily; Beddingfield, Frederick C

    2013-08-01

    Hypotrichosis of the eyelashes may negatively influence an individual's self-perception and appearance. Assessing the impact of treatment from a patient's perspective may be particularly relevant in trials of aesthetic agents. Once-daily dermal (topically applied) administration of bimatoprost ophthalmic solution 0.03% has been associated with increased eyelash prominence (ie, length, thickness, darkness). The authors assess patient-reported outcomes (PRO) after treatment with bimatoprost for hypotrichosis of the eyelashes. In this multicenter, double-masked, randomized, vehicle-controlled, parallel clinical trial, 4 PRO questionnaires were distributed to 278 patients (bimatoprost [n = 137] and vehicle [n = 141]). The primary PRO questionnaire was the 23-item Eyelash Satisfaction Questionnaire (ESQ), which measured satisfaction in 3 domains: length, fullness, and overall satisfaction (LFOS); confidence, attractiveness, and professionalism (CAP); and impact on daily routine (DR). By week 16, the bimatoprost group reported significantly greater improvements from baseline on all ESQ items (P ≤ .0433). These improvements were sustained through the 4-week posttreatment study visit. Patient satisfaction was significantly greater in the bimatoprost group than in the vehicle group for all 3 domains: LFOS (weeks 8-20; P ≤ .0052), CAP (weeks 12-20; P solution 0.03% at producing more prominent eyelashes in adults.

  20. Impact of curly top host plants on accumulation, competitiveness, and durability of curtovirus species.

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    Curly top disease, caused by viruses in the genus, Curtovirus, has affected sugarbeet production throughout much of the West for over a century; however, over that period the viruses responsible for causing the disease have changed. The two curly top virus species currently affecting production, Bee...

  1. Registration of sugar beet doubled haploid line KDH13 with resistance to beet curly top

    Science.gov (United States)

    KDH13 is a sugar beet (Beta vulgaris L. ssp vulgaris) doubled haploid line (PI 663862) released as a genetic stock by USDA-ARS in cooperation with the Beet Sugar Development Foundation, Denver, CO. KDH13 is resistant to beet curly top (BCT) caused by Beet curly top virus which is transmitted by the ...

  2. Foliar insecticides for the control of curly top in Idaho sugar beet, 2017

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    Curly top caused by Beet curly top virus (BCTV) is a widespread disease problem vectored by the beet leafhopper in semiarid sugar beet production areas. Host resistance is the primary defense against this problem, but resistance in commercial cultivars is only low to intermediate. The neonicotiono...

  3. Eyelash Epilation in the Absence of Trichiasis: Results of a Population-Based Prevalence Survey in the Western Division of Fiji.

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    Colin Macleod

    2017-01-01

    Full Text Available The WHO definition of trachomatous trichiasis (TT is "at least one eyelash touching the globe, or evidence of recent epilation of in-turned eyelashes", reflecting the fact that epilation is used as a self-management tool for TT. In Fiji's Western Division, a high TT prevalence (8.7% in those aged ≥15 years was reported in a 2012 survey, yet a 2013 survey found no TT and Fijian ophthalmologists rarely see TT cases. Local anecdote suggests that eyelash epilation is a common behaviour, even in the absence of trichiasis. Epilators may have been identified as TT cases in previous surveys.We used a preliminary focus group to design an interview questionnaire, and subsequently conducted a population-based prevalence survey to estimate the prevalence of epilation in the absence of trichiasis, and factors associated with this behaviour, in the Western Division of Fiji.We sampled 695 individuals aged ≥15 years from a total of 457 households in 23 villages. 125 participants (18% reported epilating their eyelashes at least once within the past year. Photographs were obtained of the eyes of 121/125 (97% individuals who epilated, and subsequent analysis by an experienced trachoma grader found no cases of trachomatous conjunctival scarring or trichiasis. The age- and sex- adjusted prevalence of epilation in those aged ≥15 years was 8.6% (95% CI 5.7-11.3%. iTaukei ethnicity, female gender, and a higher frequency of drinking kava root were independently associated with epilation.Epilation occurs in this population in the absence of trichiasis, with sufficient frequency to have markedly inflated previous estimates of local TT prevalence. Individuals with epilated eyelashes should be confirmed as having epilated in-turned eyelashes in an eye with scarring of the conjunctiva before being counted as cases of TT.

  4. Eyelash Epilation in the Absence of Trichiasis: Results of a Population-Based Prevalence Survey in the Western Division of Fiji.

    Science.gov (United States)

    Macleod, Colin; Yalen, Chelsea; Butcher, Robert; Mudaliar, Umesh; Natutusau, Kinisimere; Rainima-Qaniuci, Mere; Haffenden, Chris; Watson, Conall; Cocks, Naomi; Cikamatana, Luisa; Roberts, Chrissy H; Marks, Michael; Rafai, Eric; Mabey, David C W; Kama, Mike; Solomon, Anthony W

    2017-01-01

    The WHO definition of trachomatous trichiasis (TT) is "at least one eyelash touching the globe, or evidence of recent epilation of in-turned eyelashes", reflecting the fact that epilation is used as a self-management tool for TT. In Fiji's Western Division, a high TT prevalence (8.7% in those aged ≥15 years) was reported in a 2012 survey, yet a 2013 survey found no TT and Fijian ophthalmologists rarely see TT cases. Local anecdote suggests that eyelash epilation is a common behaviour, even in the absence of trichiasis. Epilators may have been identified as TT cases in previous surveys. We used a preliminary focus group to design an interview questionnaire, and subsequently conducted a population-based prevalence survey to estimate the prevalence of epilation in the absence of trichiasis, and factors associated with this behaviour, in the Western Division of Fiji. We sampled 695 individuals aged ≥15 years from a total of 457 households in 23 villages. 125 participants (18%) reported epilating their eyelashes at least once within the past year. Photographs were obtained of the eyes of 121/125 (97%) individuals who epilated, and subsequent analysis by an experienced trachoma grader found no cases of trachomatous conjunctival scarring or trichiasis. The age- and sex- adjusted prevalence of epilation in those aged ≥15 years was 8.6% (95% CI 5.7-11.3%). iTaukei ethnicity, female gender, and a higher frequency of drinking kava root were independently associated with epilation. Epilation occurs in this population in the absence of trichiasis, with sufficient frequency to have markedly inflated previous estimates of local TT prevalence. Individuals with epilated eyelashes should be confirmed as having epilated in-turned eyelashes in an eye with scarring of the conjunctiva before being counted as cases of TT.

  5. Morphological criteria of feminine upper eyelashes, quantified by a new semi-automatized image analysis: Application to the objective assessment of mascaras.

    Science.gov (United States)

    Shaiek, A; Flament, F; François, G; Vicic, M; Cointereau-Chardron, S; Curval, E; Canevet-Zaida, S; Coubard, O; Idelcaid, Y

    2018-02-01

    The wide diversity of feminine eyelashes in shape, length, and curvature makes it a complex domain that remains to be quantified in vivo, together with their changes brought by application of mascaras that are visually assessed by women themselves or make-up experts. A dedicated software was developed to semi-automatically extract and quantify, from digital images (frontal and lateral pictures), the major parameters of feminine eyelashes of Mexican and Caucasian women and to record the changes brought by the applications of various mascaras and their brushes, being self or professionally applied. The diversity of feminine eyelashes appears as a major influencing factor in the application of mascaras and their related results. Eight marketed mascaras and their respective brushes were tested and their quantitative profiles, in terms of coverage, morphology, or curvature were assessed. Standard applications by trained aestheticians led to higher and more homogeneous deposits of mascara, as compared to those resulting from self-applications. The developed software appears a precious tool for both quantifying the major characteristics of eyelashes and assessing the making-up results brought by mascaras and their associated brushes. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  6. Confirmatory factor analysis (CFA of the Crack Use Relapse Scale (CURS

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    Rosemeri Pedroso

    2016-06-01

    Full Text Available Abstract Background When it comes to crack/drug use, relapse is a relatively common event in the first weeks after the end of treatment. However little is known about what happens to patients who relapse after discharge. Objective To report the confirmatory factor analysis (CFA of the Crack Use Relapse Scale (CURS in an inpatient population. Methods A five-point Likert scale with 25 items and, initially, 9 theoretical factors was generated and utilized in a cross-sectional study with a sample of 333 hospitalized male crack users. Results CFA indicated a well-fitting model for the CURS. Discussion The CFA shows that the CURS model is appropriate and well-fitting for assessment of latent variables common to psychiatric and psychological constructs – in this case, relapse of crack cocaine use after inpatient treatment.

  7. Transpositional inactivation of gadW enhances curli production and biofilm formation in Enterohemorrhagic Escherichia coli O157:H7

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    Enterohemorrhagic Escherichia coli (EHEC) O157:H7 has been shown to produce variants that either express or are repressed in the expression of curli fimbriae promoting bacterial attachment, aggregation, and biofilm formation. The variant expression of curli fimbriae in some instances could result fr...

  8. Neonicotinoid Seed Treatments and Foliar Sprays on Sugarbeet for Control of Severe Curly Top

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    Sugarbeet production in semiarid regions is hindered by yield loss caused with Beet severe curly top virus and other closely related species vectored by the beet leafhopper. In 2010, a study was established to investigate the level of control from seed treatments and supplemental foliar insecticide...

  9. Arabidopsis thaliana Remorins Interact with SnRK1 and Play a Role in Susceptibility to Beet Curly Top Virus and Beet Severe Curly Top Virus

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    Seungmin Son

    2014-09-01

    Full Text Available Remorins, a family of plant-specific proteins containing a variable N-terminal region and conserved C-terminal domain, play a role in various biotic and abiotic stresses, including host-microbe interactions. However, their functions remain to be completely elucidated, especially for the Arabidopsis thaliana remorin group 4 (AtREM4. To elucidate the role of remorins in Arabidopsis, we first showed that AtREM4s have typical molecular characteristics of the remorins, such as induction by various types of biotic and abiotic stresses, localization in plasma membrane and homo- and hetero-oligomeric interaction. Next, we showed that their loss-of-function mutants displayed reduced susceptibility to geminiviruses, Beet Curly Top Virus and Beet Severe Curly Top Virus, while overexpressors enhanced susceptibility. Moreover, we found that they interacted with SnRK1, which phosphorylated AtREM4.1, and were degraded by the 26S proteasome pathway. These results suggest that AtREM4s may be involved in the SnRK1-mediated signaling pathway and play a role as positive regulators of the cell cycle during geminivirus infection.

  10. A novel nanofiber Cur-loaded polylactic acid constructed by electrospinning

    International Nuclear Information System (INIS)

    Trang Mai, Thi Thu; Duong Le, Quang; Binh Nguyen, Hai; Hoa Phan, Thi Bich; Lam Tran, Dai; Nguyen, Xuan Phuc; Thuy Nguyen, Thi Thu; Seo Park, Jun; Ngoan Nguyen, Thi; Cham Ba, Thi

    2012-01-01

    Curcumin (Cur), extracted from the Curcuma longa L. plant, is well known for its anti-tumor, anti-oxidant, anti-inflammatory and anti-bacterial properties. Nanofiber mats of polylactic acid (PLA) loading Cur (5 wt%) were fabricated by electrospinning (e-spinning). Morphology and structure of the fibers were characterized by field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared (FTIR) spectroscopy, respectively. The diameters of the obtained fibers varied from 200 to 300 nm. The release capacity of curcumin from curcumin-loaded PLA fibers was investigated in phosphate buffer saline (PBS) containing ethanol. After 24 h, 50% of the curcumin was released from curcumin-loaded PLA fibers. These results of electrospun (e-spun) fibers exhibit the potential for biomedical application

  11. A novel nanofiber Cur-loaded polylactic acid constructed by electrospinning

    Science.gov (United States)

    Thu Trang Mai, Thi; Thu Thuy Nguyen, Thi; Duong Le, Quang; Ngoan Nguyen, Thi; Cham Ba, Thi; Binh Nguyen, Hai; Bich Hoa Phan, Thi; Tran, Dai Lam; Phuc Nguyen, Xuan; Park, Jun Seo

    2012-06-01

    Curcumin (Cur), extracted from the Curcuma longa L. plant, is well known for its anti-tumor, anti-oxidant, anti-inflammatory and anti-bacterial properties. Nanofiber mats of polylactic acid (PLA) loading Cur (5 wt%) were fabricated by electrospinning (e-spinning). Morphology and structure of the fibers were characterized by field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared (FTIR) spectroscopy, respectively. The diameters of the obtained fibers varied from 200 to 300 nm. The release capacity of curcumin from curcumin-loaded PLA fibers was investigated in phosphate buffer saline (PBS) containing ethanol. After 24 h, 50% of the curcumin was released from curcumin-loaded PLA fibers. These results of electrospun (e-spun) fibers exhibit the potential for biomedical application.

  12. Composition of lactic acid bacteria during spontaneous curly kale (Brassica oleracea var. sabellica) fermentation.

    Science.gov (United States)

    Michalak, Magdalena; Gustaw, Klaudia; Waśko, Adam; Polak-Berecka, Magdalena

    2018-01-01

    The present work is the first report on spontaneous fermentation of curly kale and characteristics of autochthonous lactic acid bacteria (LAB). Our results indicate that curly kale fermentation is the new possibility of the technological use of this vegetable. Bacteria representing ten different species were isolated from three phases of curly kale fermentation and identified by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Among them, four species were identified as Lactobacillus spp. (Lb. plantarum 332, Lb. paraplantarum G2114, Lb. brevis R413, Lb. curvatus 154), two as Weissella spp. (W. hellenica 152, W. cibaria G44), two as Pediococcus spp. (P. pentosaceus 45AN, P. acidilactici 2211), one as Leuconostoc mesenteroides 153, and one as Lactococcus lactis 37BN. The functional properties of isolates, i.e. acid, NaCl and bile salt tolerance, enzyme activities, adhesion to hydrocarbons, and antibiotic resistance, were examined. Among the tested strains, Lb. plantarum 332, Lb. paraplantarum G2114, P. pentosaceus 2211, and Lb. brevis R413 exhibited the best hydrophobicity value and high tolerance to bile salts, NaCl, and low pH. Copyright © 2017 Elsevier GmbH. All rights reserved.

  13. Clinical and pathological features of hair coat abnormalities in curly coated retrievers from UK and Sweden.

    Science.gov (United States)

    Bond, R; Varjonen, K; Hendricks, A; Chang, Y M; Brooks Brownlie, H

    2016-12-01

    To gain information on hair loss amongst curly coated retrievers by questionnaire and to define the clinical and pathological features of hair coat abnormalities in affected dogs in the United Kingdom and Sweden. Questionnaires were completed by members of the Curly Coated Retriever Clubs. Fourteen dogs (six in the United Kingdom, eight in Sweden) were clinically examined and skin/hair samples collected for microscopy and histopathology. Blood was collected for haematological, biochemical and endocrine assays. Of 90 dogs surveyed, 39 had current or previous episodes of symmetrical, non-pruritic alopecia and or frizzy coat changes, usually affecting caudal thighs, axillae, dorsum and neck before 18 months of age; 23 dogs had a waxing/waning course. Examined dogs generally matched the pattern described in questionnaires. Hair shaft anomalies comprised occasional distorted anagen bulbs (10 dogs) and transverse fractures (8 dogs). Vertical histopathological sections showed infundibular hyperkeratosis (28 of 30 sections) and low-grade pigment clumping (17 of 30). Subtle telogenisation of hair follicles was unequivocally confirmed by transverse histomorphometric analyses. The follicular dysplasia of curly coated retriever reported here is similar to that of Irish water spaniels and Chesapeake Bay retrievers but distinct from that of Portuguese water dogs. The genetic basis requires further assessment. © 2016 British Small Animal Veterinary Association.

  14. Control of Paternally Expressed Imprinted UPWARD CURLY LEAF1, a Gene Encoding an F-Box Protein That Regulates CURLY LEAF Polycomb Protein, in the Arabidopsis Endosperm.

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    Cheol Woong Jeong

    Full Text Available Genomic imprinting, an epigenetic process in mammals and flowering plants, refers to the differential expression of alleles of the same genes in a parent-of-origin-specific manner. In Arabidopsis, imprinting occurs primarily in the endosperm, which nourishes the developing embryo. Recent high-throughput sequencing analyses revealed that more than 200 loci are imprinted in Arabidopsis; however, only a few of these imprinted genes and their imprinting mechanisms have been examined in detail. Whereas most imprinted loci characterized to date are maternally expressed imprinted genes (MEGs, PHERES1 (PHE1 and ADMETOS (ADM are paternally expressed imprinted genes (PEGs. Here, we report that UPWARD CURLY LEAF1 (UCL1, a gene encoding an E3 ligase that degrades the CURLY LEAF (CLF polycomb protein, is a PEG. After fertilization, paternally inherited UCL1 is expressed in the endosperm, but not in the embryo. The expression pattern of a β-glucuronidase (GUS reporter gene driven by the UCL1 promoter suggests that the imprinting control region (ICR of UCL1 is adjacent to a transposable element in the UCL1 5'-upstream region. Polycomb Repressive Complex 2 (PRC2 silences the maternal UCL1 allele in the central cell prior to fertilization and in the endosperm after fertilization. The UCL1 imprinting pattern was not affected in paternal PRC2 mutants. We found unexpectedly that the maternal UCL1 allele is reactivated in the endosperm of Arabidopsis lines with mutations in cytosine DNA METHYLTRANSFERASE 1 (MET1 or the DNA glycosylase DEMETER (DME, which antagonistically regulate CpG methylation of DNA. By contrast, maternal UCL1 silencing was not altered in mutants with defects in non-CpG methylation. Thus, silencing of the maternal UCL1 allele is regulated by both MET1 and DME as well as by PRC2, suggesting that divergent mechanisms for the regulation of PEGs evolved in Arabidopsis.

  15. The Geosciences Division of the Council on Undergraduate Research (GeoCUR): Supporting Faculty that Mentor Undergraduate Researchers

    Science.gov (United States)

    Fox, L. K.; Guertin, L. A.; Manley, P. L.; Fortner, S. K.

    2012-12-01

    Undergraduate research is a proven effective pedagogy that has a number of benefits including: enhancing student learning through mentoring relationships with faculty; increasing retention; increasing enrollment in graduate programs; developing critical thinking, creativity, problem solving and intellectual independence; and, developing an understanding of research methodology. Undergraduate research also has been demonstrated in preparing students for careers. In addition to developing disciplinary and technical expertise, participation in undergraduate research helps students improve communication skills (written, oral, and graphical) and time management. Early involvement in undergraduate research improves retention and, for those engaged at the 2YC level, helps students successfully transfers to 4YC. The Geosciences Division of the Council on Undergraduate Research (GeoCUR) supports faculty in their development of undergraduate research programs at all levels. GeoCUR leads workshops for new and future faculty covering all aspects of undergraduate research including incorporating research into coursework, project design, mentoring students, sustaining programs, and funding sources. GeoCUR members support new faculty by providing a range of services including: peer-review of grant proposals; advice on establishing an undergraduate research program; balancing teaching and research demands; and networking with other geoscientist. GeoCUR has also developed web resources that support faculty and departments in development of undergraduate research programs (http://serc.carleton.edu/NAGTWorkshops/undergraduate_research/index.html). This presentation will describe the services provided by GeoCUR and highlight examples of programs and resources available to geoscientists in all career stages for effective undergraduate research mentoring and development.

  16. Heat transfer nanofluid based on curly ultra-long multi-wall carbon nanotubes

    Science.gov (United States)

    Boncel, Sławomir; Zniszczoł, Aurelia; Pawlyta, Mirosława; Labisz, Krzysztof; Dzido, Grzegorz

    2018-02-01

    The main challenge in the use of multi-wall carbon nanotube (MWCNT) as key components of nanofluids is to transfer excellent thermal properties from individual nanotubes into the bulk systems. We present studies on the performance of heat transfer nanofluids based on ultra-long ( 2 mm), curly MWCNTs - in the background of various other nanoC-sp2, i.e. oxidized MWCNTs, commercially available Nanocyl™ MWCNTs and spherical carbon nanoparticles (SCNs). The nanofluids prepared via ultrasonication from water and propylene glycol were studied in terms of heat conductivity and heat transfer in a scaled up thermal circuit containing a copper helical heat exchanger. Ultra-long curly MWCNT (1 wt.%) nanofluids (stabilized with Gum Arabic in water) emerged as the most thermally conducting ones with a 23-30%- and 39%-enhancement as compared to the base-fluids for water and propylene glycol, respectively. For turbulent flows ( Re = 8000-11,000), the increase of heat transfer coefficient for the over-months stable 1 wt.% ultra-long MWCNT nanofluid was found as high as >100%. The findings allow to confirm that longer MWCNTs are promising solid components in nanofluids and hence to predict their broader application in heat transfer media.

  17. The DigCurV Curriculum Framework for Digital Curation in the Cultural Heritage Sector

    Directory of Open Access Journals (Sweden)

    Laura Molloy

    2014-07-01

    Full Text Available In 2013, the DigCurV collaborative network completed development of a Curriculum Framework for digital curation skills in the European cultural heritage sector. DigCurV synthesised a variety of established skills and competence models in the digital curation and LIS sectors with expertise from digital curation professionals, in order to develop a new Curriculum Framework. The resulting Framework provides a common language and helps define the skills, knowledge and abilities that are necessary for the development of digital curation training; for benchmarking existing programmes; and for promoting the continuing production, improvement and refinement of digital curation training programmes. This paper describes the salient points of this work, including how the project team conducted the research necessary to develop the Framework, the structure of the Framework, the processes used to validate the Framework, and three ‘lenses’ onto the Framework. The paper also provides suggestions as to how the Framework might be used, including a description of potential audiences and purposes.

  18. Expression of curli by Escherichia coli O157:H7 strains isolated from patients during outbreaks is different from similar strains isolated from leafy green production environments

    Directory of Open Access Journals (Sweden)

    Subbarao Venkata Ravva

    2016-12-01

    Full Text Available We previously reported that the strains of Escherichia coli O157:H7 (EcO157 that survived longer in austere soil environment lacked expression of curli, a fitness trait linked with intestinal colonization. In addition, the proportion of curli-positive variants of EcO157 decreased with repeated soil exposure. Here we evaluated 84 and 176 clinical strains from outbreaks and sporadic infections in the US, plus 211 animal fecal and environmental strains for curli expression. These shiga-toxigenic strains were from 328 different genotypes, as characterized by multi-locus variable-number tandem-repeat analysis (MLVA. More than half of the fecal strains (human and animal and a significant proportion of environmental isolates (82% were found to lack curli expression. EcO157 strains from several outbreaks linked with the consumption of contaminated apple juice, produce, hamburgers, steak and beef were also found to lack curli expression. Phylogenetic analysis of fecal strains indicates curli expression is distributed throughout the population. However, a significant proportion of animal fecal isolates (84% gave no curli expression compared to human fecal isolates (58%. In addition, analysis of environmental isolates indicated nearly exclusive clustering of curli expression to a single branch of the minimal spanning tree. This indicates that curli expression depends primarily upon the type of environmental exposure and the isolation source, although genotypic differences also contribute to clonal variation in curli. Furthermore, curli-deficient phenotype appears to be a selective trait for survival of EcO157 in agricultural environments.

  19. Defective DNAs of beet curly top virus from long-term survivor sugar beet plants.

    Science.gov (United States)

    Bach, Judith; Jeske, Holger

    2014-04-01

    Long-term surviving sugar beet plants were investigated after beet curly top virus infection to characterize defective (D) viral DNAs as potential symptom attenuators. Twenty or 14 months after inoculation, 20 D-DNAs were cloned and sequenced. In contrast to known D-DNAs, they exhibited a large range of sizes. Deletions were present in most open reading frames except ORF C4, which encodes a pathogenicity factor. Direct repeats and inverted sequences were observed. Interestingly, the bidirectional terminator of transcription was retained in all D-DNAs. A model is presented to explain the deletion sites and sizes with reference to the viral minichromosome structure, and symptom attenuation by D-DNAs is discussed in relation to RNA interference. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Acute oxalate poisoning attributable to ingestion of curly dock (Rumex crispus) in sheep.

    Science.gov (United States)

    Panciera, R J; Martin, T; Burrows, G E; Taylor, D S; Rice, L E

    1990-06-15

    Ten of 100 mature ewes were afflicted with acute oxalate toxicosis within 40 hours after being temporarily penned in a lot that contained considerable growing Rumex crispus (curly dock). Clinical signs of toxicosis included excess salivation, tremors, ataxia, and recumbency. Affected ewes were markedly hypocalcemic and azotemic. Oxalate crystals were not observed in urine. Gross postmortem lesions were minimal and nondiagnostic in 2 ewes that died peracutely, but perirenal edema and renal tubular degeneration were clearly observable in ewes euthanatized on the third day of toxicosis. Diagnosis of oxalate toxicosis was confirmed by histopathologic findings. Samples of Rumex spp contained 6.6 to 11.1% oxalic acid on a dry-weight basis, a concentration comparable with that in other oxalate-containing plants that have caused acute oxalate toxicosis.

  1. Involvement of C4 protein of beet severe curly top virus (family Geminiviridae in virus movement.

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    Kunling Teng

    Full Text Available BACKGROUND: Beet severe curly top virus (BSCTV is a leafhopper transmitted geminivirus with a monopartite genome. C4 proteins encoded by geminivirus play an important role in virus/plant interaction. METHODS AND FINDINGS: To understand the function of C4 encoded by BSCTV, two BSCTV mutants were constructed by introducing termination codons in ORF C4 without affecting the amino acids encoded by overlapping ORF Rep. BSCTV mutants containing disrupted ORF C4 retained the ability to replicate in Arabidopsis protoplasts and in the agro-inoculated leaf discs of N. benthamiana, suggesting C4 is not required for virus DNA replication. However, both mutants did not accumulate viral DNA in newly emerged leaves of inoculated N. benthamiana and Arabidopsis, and the inoculated plants were asymptomatic. We also showed that C4 expression in plant could help C4 deficient BSCTV mutants to move systemically. C4 was localized in the cytosol and the nucleus in both Arabidopsis protoplasts and N. benthamiana leaves and the protein appeared to bind viral DNA and ds/ssDNA nonspecifically, displaying novel DNA binding properties. CONCLUSIONS: Our results suggest that C4 protein in BSCTV is involved in symptom production and may facilitate virus movement instead of virus replication.

  2. Managing female urinary incontinence: A regional prospective analysis of cost-utility ratios (curs and effectiveness

    Directory of Open Access Journals (Sweden)

    Elisabetta Costantini

    2014-06-01

    Full Text Available Introduction: To evaluate the cost-utility of incontinence treatments, particularly anticholinergic therapy, by examining costs and quality-adjusted life years. Materials and methods: A prospective cohort study of women who were consecutively referred by general practitioners (GPs to the Urology Department because of urinary incontinence. The primary outcome was evaluation of the cost-utility of incontinence treatments (surgery, medical therapy and physiotherapy for stress and/or urgency incontinence by examining costs and quality-adjusted life years. Results: 137 consecutive female patients (mean age 60.6 ± 11.6; range 36-81 were enrolled and stratified according to pathologies: SUI and UUI. Group A: SUI grade II-III: 43 patients who underwent mid-urethral sling (MUS; Group B: SUI grade I-II 57 patients who underwent pelvic floor muscle exercise and Group C: UUI: 37 patients who underwent antimuscarinic treatment with 5 mg solifenacin daily. The cost utility ratio (CUR was estimated as saving more than €1200 per QALY for surgery and physiotherapy and as costing under € 100 per QALY for drug therapy. Conclusions: This study shows that appropriate diagnosis and treatment of a patient with incontinence lowers National Health Service costs and improves the benefits of treatment and quality of life.

  3. TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity

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    Bo Hou

    2014-01-01

    Full Text Available While a high osmolarity medium activates Cpx signaling and causes CpxR to repress csgD expression, and efflux protein TolC protein plays an important role in biofilm formation in Escherichia coli, whether TolC also responds to an osmolarity change to regulate biofilm formation in extraintestinal pathogenic E. coli (ExPEC remains unknown. In this study, we constructed ΔtolC mutant and complement ExPEC strains to investigate the role of TolC in the retention of biofilm formation and curli production capability under different osmotic conditions. The ΔtolC mutant showed significantly decreased biofilm formation and lost the ability to produce curli fimbriae compared to its parent ExPEC strain PPECC42 when cultured in M9 medium or 1/2 M9 medium of increased osmolarity with NaCl or sucrose at 28°C. However, biofilm formation and curli production levels were restored to wild-type levels in the ΔtolC mutant in 1/2 M9 medium. We propose for the first time that TolC protein is able to form biofilm even under high osmotic stress. Our findings reveal an interplay between the role of TolC in ExPEC biofilm formation and the osmolarity of the surrounding environment, thus providing guidance for the development of a treatment for ExPEC biofilm formation.

  4. Correcting Inconsistencies and Errors in Bacterial Genome Metadata Using an Automated Curation Tool in Excel (AutoCurE).

    Science.gov (United States)

    Schmedes, Sarah E; King, Jonathan L; Budowle, Bruce

    2015-01-01

    Whole-genome data are invaluable for large-scale comparative genomic studies. Current sequencing technologies have made it feasible to sequence entire bacterial genomes with relative ease and time with a substantially reduced cost per nucleotide, hence cost per genome. More than 3,000 bacterial genomes have been sequenced and are available at the finished status. Publically available genomes can be readily downloaded; however, there are challenges to verify the specific supporting data contained within the download and to identify errors and inconsistencies that may be present within the organizational data content and metadata. AutoCurE, an automated tool for bacterial genome database curation in Excel, was developed to facilitate local database curation of supporting data that accompany downloaded genomes from the National Center for Biotechnology Information. AutoCurE provides an automated approach to curate local genomic databases by flagging inconsistencies or errors by comparing the downloaded supporting data to the genome reports to verify genome name, RefSeq accession numbers, the presence of archaea, BioProject/UIDs, and sequence file descriptions. Flags are generated for nine metadata fields if there are inconsistencies between the downloaded genomes and genomes reports and if erroneous or missing data are evident. AutoCurE is an easy-to-use tool for local database curation for large-scale genome data prior to downstream analyses.

  5. Characteristics and Expression Profile of KRT71 Screened by Suppression Subtractive Hybridization cDNA Library in Curly Fleece Chinese Tan Sheep.

    Science.gov (United States)

    Kang, Xiaolong; Liu, Yufang; Zhang, Jibin; Xu, Qinqin; Liu, Chengkun; Fang, Meiying

    2017-07-01

    As an important commercial trait for sheep, curly fleece has a great economic impact on production costs and efficiency in sheep industry. To identify genes that are important for curly fleece formation in mammals, a suppression subtractive hybridization analysis was performed on the shoulder skin tissues exposed to two different growth stages of Chinese Tan sheep with different phenotypes (curly fleece and noncurling fleece). BLAST analysis identified 67 differentially expressed genes, of which 31 were expressed lower and 36 were expressed higher in lambs than in adult sheep. Differential expressions of seven randomly selected genes were verified using quantitative real-time polymerase chain reaction (qRT-PCR). KRT71 gene was selected for further study due to its high correlation with the curly hair phenotype in various mammal species. Semi-qPCR showed distinctively high expression of KRT71 in skin tissues. Moreover, qPCR result showed a significantly higher expression of KRT71 in curly fleece than noncurling Tan sheep. The luciferase assay and electrophoresis mobility shift assay showed that there were transcription factor binding sites in the promoter region of KRT71 related to the differential expression of KRT71 at the two growth stages of Tan sheep. Online bioinformation tools predicted MFZ1 as a transcriptional factor that regulates the expression of KRT71. These studies on KRT71 gene revealed some mechanisms underlying the relationship between the KRT71 gene and the curly fleece phenotype of Tan sheep.

  6. AP2/ERF Transcription Factors Involved in Response to Tomato Yellow Leaf Curly Virus in Tomato

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    Ying Huang

    2016-07-01

    Full Text Available Tomato yellow leaf curly virus (TYLCV, transmitted by the whitefly (, causes leaf curling and yellowing, plant dwarfism, and growth inhibition in tomato ( L.. The APETALA2 (AP2 and ethylene response factor (ERF transcription factor (TF family, the largest plant-specific TF family, was identified to function in plant development and pathogen defense. Our study aimed to analyze the mechanism underlying the function of ERF (SlERF TFs in response to TYLCV infection and improve useful information to increase the resistance to TYLCV in tomato. A total of 22 tomato AP2/ERF TFs in response to TYLCV were identified according to transcriptome database. Five ERF-B3 TFs were identified in cultivars Hongbeibei (highly resistant, Zheza-301, Zhefen-702 (both resistant, Jinpeng-1, and Xianke-6 (both susceptible. Interaction network indicated that SlERF TFs could interact with mitogen-activated protein kinase (MAPK. Expression profiles of five ERF-B3 genes (, , , , and were detected by quantitative real-time–polymerase chain reaction (qRT-PCR after TYLCV infection in five tomato cultivars. expression was upregulated in five tomato cultivars. The expressions of three genes (, , and were upregulated in Zheza-301 and Zhefen-702. and expressions were downregulated in Hongbeibei and Xianke-6, respectively. Yeast one-hybrid showed that the GCC-box binding ability of ERF-B3 TFs differed in resistant and susceptible tomato cultivars. Expression profiles were related to the GCC-box binding ability of SlERF TFs in resistant and susceptible tomato cultivars. The defense mechanism underlying the tomato’s response to TYLCV involved a complicated network, which provided important information for us in breeding and genetic analysis.

  7. Short communication. Effect of deficit irrigation on curly lettuce grown under semiarid conditions

    Energy Technology Data Exchange (ETDEWEB)

    Kuslu, Y.; Dursun, A.; Sahin, U.; Kiziloglu, F. M.; Turan, M.

    2008-07-01

    Field experiments were conducted to characterize the effects of deficit irrigation on curly lettuce (Lactuca sativa var. Crispa cv. Bohemia) evapotranspiration, water use efficiency, marketable yield, yield components and mineral contents. The experiments were performed under semiarid climatic conditions in Erzurum province (east of Turkey) in the summer periods of 2005 and 2006. Irrigation water levels were selected to be 100% of usable soil water in full irrigation treatment (control) (T-100) and 80%, 60%, 40% and 20% of usable soil water in deficit irrigation treatments (T-80, T-60, T-40 and T- 20, respectively). Average seasonal evapotranspiration was 232 mm in T-100 and 121 mm in T-20. Average marketable yield was 39.49 Mg ha{sup -}1 in T-100 and 14.57 Mg ha{sup -}1 in T-20. A linear relationship (y=0.23x-13.97; R{sup 2}0.94) was found between seasonal evapotranspiration (x) and marketable plant yield (y). According to the regression equation, the yield response factor (k{sub y}) was found to be 1.39, and the coefficient of determination 0.91. Average water use efficiency was 168.88 kg ha{sup -}1 mm{sup -}1 in T-100 and 117.39 kg ha{sup -}1 mm{sup -}1 in T-20. The lowest plant length, width, steam diameter, leaf number, macro and micro element content values were obtained for T-20 in both years. (Author) 21 refs.

  8. El clasicismo greco-romano en «La Curée», de Zola

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    Luis LÓPEZ JIMÉNEZ

    2009-08-01

    Full Text Available Cuando Zola concibió La Curée, no parece que pensara inicialmente en la antigüedad clásica. En 1869, tres años antes de la publicación, dijo al editor que trataría en su novela «de la vida estúpida y de crápula elegante de nuestros jóvenes ricos» y «de las turbias y desenfrenadas especulaciones del Segundo Imperio»1. La idea de lo clásico debió de presentársele luego, al introducir el incesto, que le traería inmediatamente a la mente la tragedia de Racine Fedra (1677, inspirada en la griega de Eurípides (s. V a. de J.C. y en la latina del cordobés Séneca (s. I d. de J.C; se le iría imponiendo esa idea de introducir lo clásico en la novela, movido por la fuerza poética del tema y el prestigio literario y artístico de la antigüedad greco-latina. Obedecería, pues, en un principio a su propósito de reflejar un sector de la sociedad pudiente corrompida de París bajo Napolón III, y sólo más tarde forzó la comparación con el tema de Fedra, introdujo la fábula de Eco y Narciso y añadió otros elementos ambientales y literarios de la antigüedad clásica

  9. The impact of sesquiterpene lactones and phenolics on sensory attributes: An investigation of a curly endive and escarole germplasm collection.

    Science.gov (United States)

    Filippo D'Antuono, L; Ferioli, Federico; Manco, Manuela Agata

    2016-05-15

    In the present study, curly endive (Cichorium endivia L. var. crispum) and escarole (Cichorium endivia L. var. latifolium) accessions were investigated for their sensory characters (bitterness, astringency and herbaceous flavour) and acceptance in relation to sesquiterpene lactone and phenolic content. Different facets of the perception of these sensory traits in relation to lactones and phenolics were brought out. Lactucopicrin and kaempferol malonyl glucoside were consistently related to bitterness, astringency and herbaceous flavour perceptions. Overall acceptance was significantly and inversely related mainly to bitterness. The generic statement that sesquiterpene lactones and phenolic compounds are determinants of bitterness and other related sensory characters does not seem to be fully consistent with our data, that indicated how the balance of different compounds affects these traits individually, in a rather complex manner, with a prevailing negative impact of phenolics. Bitter, astringent, and herbaceous perceptions were significantly affected by variety, with curly endive showing on average higher scores in comparison to escarole, with particular respect to bitterness. Copyright © 2015 Elsevier Ltd. All rights reserved.

  10. Distribution and Rate of Movement of the Curtovirus Beet mild curly top virus (Family Geminiviridae) in the Beet Leafhopper.

    Science.gov (United States)

    Soto, Maria J; Gilbertson, Robert L

    2003-04-01

    ABSTRACT A polymerase chain reaction (PCR)-based method for the detection of the curtovirus Beet mild curly top virus (BMCTV, previously named the Worland strain of Beet curly top virus) was developed and used to investigate the BMCTV-beet leafhopper interaction. Using PCR and a BMCTV-specific primer pair, an approximately 1.1-kb BMCTV DNA fragment was amplified from adult leafhoppers and from the organs involved in circulative transmission: the digestive tract, hemolymph, and salivary glands. The temporal distribution of BMCTV in the leafhopper was determined using insects given acquisition access periods (AAPs) ranging from 1 to 48 h on BMCTV-infected shepherd's purse plants. BMCTV was detected in the digestive tract after all AAPs, in the hemolymph after AAPs of 3 h or greater, and in the salivary glands after AAPs of 4 h or greater. The amount of virus detected in the hemolymph and salivary glands increased with AAP length. The virus persisted for up to 30 days in leafhoppers (given a 3-day AAP on BMCTV-infected plants) maintained on corn plants, a nonhost for BMCTV, but transovarial transmission was not detected. These results are consistent with a persistent but nonpropagative mode of circulative transmission.

  11. Influence of the amyloid dye Congo red on curli, cellulose, and the extracellular matrix in E. coli during growth and matrix purification.

    Science.gov (United States)

    Reichhardt, Courtney; McCrate, Oscar A; Zhou, Xiaoxue; Lee, Jessica; Thongsomboon, Wiriya; Cegelski, Lynette

    2016-11-01

    Microbial biofilms are communities of cells characterized by a hallmark extracellular matrix (ECM) that confers functional attributes to the community, including enhanced cohesion, adherence to surfaces, and resistance to external stresses. Understanding the composition and properties of the biofilm ECM is crucial to understanding how it functions and protects cells. New methods to isolate and characterize ECM are emerging for different biofilm systems. Solid-state nuclear magnetic resonance was used to quantitatively track the isolation of the insoluble ECM from the uropathogenic Escherichia coli strain UTI89 and understand the role of Congo red in purification protocols. UTI89 assembles amyloid-integrated biofilms when grown on YESCA nutrient agar. The ECM contains curli amyloid fibers and a modified form of cellulose. Biofilms formed by UTI89 and other E. coli and Salmonella strains are often grown in the presence of Congo red to visually emphasize wrinkled agar morphologies and to score the production of ECM. Congo red is a hallmark amyloid-binding dye and binds to curli, yet also binds to cellulose. We found that growth in Congo red enabled more facile extraction of the ECM from UTI89 biofilms and facilitates isolation of cellulose from the curli mutant, UTI89ΔcsgA. Yet, Congo red has no influence on the isolation of curli from curli-producing cells that do not produce cellulose. Sodium dodecyl sulfate can remove Congo red from curli, but not from cellulose. Thus, Congo red binds strongly to cellulose and possibly weakens cellulose interactions with the cell surface, enabling more complete removal of the ECM. The use of Congo red as an extracellular matrix purification aid may be applied broadly to other organisms that assemble extracellular amyloid or cellulosic materials. Graphical abstract Solid-state NMR was used to quantitatively track the isolation of the insoluble amyloid-associated ECM from uropathogenic E. coli and understand the role of Congo red in

  12. Differential expression of CURS gene during various growth stages, climatic condition and soil nutrients in turmeric (Curcuma longa): Towards site specific cultivation for high curcumin yield.

    Science.gov (United States)

    Sandeep, I Sriram; Das, Suryasnata; Nasim, Noohi; Mishra, Antaryami; Acharya, Laxmikanta; Joshi, Raj Kumar; Nayak, Sanghamitra; Mohanty, Sujata

    2017-09-01

    Curcuma longa L., accumulates substantial amount of curcumin and essential oil. Little is known about the differential expression of curcumin synthase (CURS) gene and consequent curcumin content variations at different agroclimatic zones. The present study aimed to evaluate the effect of climate, soil and harvesting phase on expression of CURS gene for curcumin yield in two high yielding turmeric cultivars. Expression of CURS gene at different experimental zones as well as at different harvesting phase was studied through transcriptional analysis by qRT-PCR. Curcumin varied from 1.5 to 5% and 1.4-5% in Surama and Roma respectively. The expression of CURS also varied from 0.402 to 5.584 fold in Surama and 0.856-5.217 fold in Roma. Difference in curcumin content at a particular zone varied among different harvesting period from 3.95 to 4.31% in Surama and 3.57-3.83% in Roma. Expression of CURS gene was also effected by harvesting time of the rhizome which varied from 7.389 to 16.882 fold in Surama and 4.41-8.342 fold in Roma. The CURS gene expression was found regardless of variations in curcumin content at different experimental zones. This may be due to the effects of soil and environmental variables. Expression was positively correlated with curcumin content with different harvesting time at a particular zone. This find indicates effect of soil and environment on molecular and biochemical dynamics of curcumin biosynthesis and could be useful in genetic improvement of turmeric. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  13. Expression and activity of sulfate transporters and APS reductase in curly kale in response to sulfate deprivation and re-supply

    NARCIS (Netherlands)

    Koralewska, Aleksandra; Buchner, Peter; Stuiver, C. Elisabeth E.; Posthumus, Freek S.; Kopriva, Stanislav; Hawkesford, Malcolm J.; De Kok, Luit J.

    2009-01-01

    Both activity and expression of sulfate transporters and APS reductase in plants are modulated by the sulfur status of the plant. To examine the regulatory mechanisms in curly kale (Brossica olerracea L.), the sulfate supply was manipulated by the transfer of seedlings to sulfate-deprived

  14. Transfer of Salmonella enterica serovar Typhimurium from contaminated irrigation water to parsley is dependent on curli and cellulose, the biofilm matrix components.

    Science.gov (United States)

    Lapidot, Anat; Yaron, Sima

    2009-03-01

    Enteric pathogens can contaminate fresh produce, and this contaminated produce can be a significant potential source of human illness. The objective of this study was to determine a possible mode of transfer of Salmonella Typhimurium from contaminated irrigation water to mature parsley plants and to investigate the role of bacterial cellulose and curli. Parsley plants were drip irrigated with water containing green fluorescent protein-labeled Salmonella Typhimurium. Stems and leaves were harvested 1 day after the third irrigation and examined for the presence of Salmonella Typhimurium. Three weeks after harvesting, the presence of Salmonella was again confirmed in the regrown plants. During this period, bacterial numbers on leaves declined from 4.1 (+/- 0.3) to 2.3 (+/- 0.1) log CFU g(-1) (P edible parts of the plants. Confocal laser scanning microscopic images revealed that Salmonella Typhimurium formed aggregates at a depth of 8 to 32 microm beneath the leaf surface. Penetration might be achieved through the roots or the phyllosphere. The importance of the bacterial cellulose and curli was determined by comparing the wild-type strain with its mutants, which lack the ability to synthesize cellulose and curli. Counts of the double mutant were 2-log higher in the soil but 1-log lower in the leaves (P < 0.05). Deletion of the agfBA gene (for curli) was more effective than deletion of bcsA (for cellulose). Thus, curli and cellulose play a role in the transfer or survival of Salmonella Typhimurium in the plant, as they do for plant pathogens.

  15. El volcanismo bimodal del volcán cerro Corona, Alta Sierra de Somún Curá, provincia de Río Negro Bimodal volcanism of the Cerro Corona volcano in the Alta Sierra de Somún Curá (Río Negro province)

    OpenAIRE

    Guadalupe Maro; Marcela B Remesal

    2012-01-01

    La Alta Sierra de Somún Curá es uno de los complejos volcánicos terciarios postplateau en el ambiente de la meseta basáltica de Somún Curá, en la Patagonia extrandina. En ella se encuentra el cerro Corona, un volcán en escudo que corresponde al centro de mayor altura en la región. Está construido por rocas efusivas y piroclásticas subordinadas, fundamentalmente traquiandesíticas-basálticas, con variaciones traquíticas tanto en sus flancos como en su cráter. A pesar de la bimodalidad compositi...

  16. The curli regulator CsgD mediates stationary phase counter-silencing of csgBA in Salmonella Typhimurium.

    Science.gov (United States)

    Newman, S L; Will, W R; Libby, S J; Fang, F C

    2018-04-01

    Integration of horizontally acquired genes into transcriptional networks is essential for the regulated expression of virulence in bacterial pathogens. In Salmonella enterica, expression of such genes is repressed by the nucleoid-associated protein H-NS, which recognizes and binds to AT-rich DNA. H-NS-mediated silencing must be countered by other DNA-binding proteins to allow expression under appropriate conditions. Some genes that can be transcribed by RNA polymerase (RNAP) associated with the alternative sigma factor σ S or the housekeeping sigma factor σ 70 in vitro appear to be preferentially transcribed by σ S in the presence of H-NS, suggesting that σ S may act as a counter-silencer. To determine whether σ S directly counters H-NS-mediated silencing and whether co-regulation by H-NS accounts for the σ S selectivity of certain promoters, we examined the csgBA operon, which is required for curli fimbriae expression and is known to be regulated by both H-NS and σ S . Using genetics and in vitro biochemical analyses, we found that σ S is not directly required for csgBA transcription, but rather up-regulates csgBA via an indirect upstream mechanism. Instead, the biofilm master regulator CsgD directly counter-silences the csgBA promoter by altering the DNA-protein complex structure to disrupt H-NS-mediated silencing in addition to directing the binding of RNAP. © 2018 John Wiley & Sons Ltd.

  17. Curly Top of Tomato

    OpenAIRE

    Heflebower, Rick; Reid, Chad; Frank, Erin; Evans, Kent

    2008-01-01

    Crickets are closely related to grasshoppers and katydids, all belonging in the order Orthoptera. The family of “true crickets” is called Gryllidae, which includes more than 900 different species worldwide. In Utah, we have several species of field crickets and snowy tree crickets located throughout the state. True crickets are often confused with grasshoppers and katydids because they have similar body shapes and large hind legs for jumping.

  18. Impact of some Environmental Factors on Germination and Emergence Characteristics of Black Henbane (Hyoscyamus niger, Ground Cherry (Physalis divaricata and Curly Dock (Rumex crispus

    Directory of Open Access Journals (Sweden)

    R Gorbani

    2016-01-01

    Full Text Available To understand germination and emergence characteristics of black henbane, ground cherry and curly dock a laboratory experiment, based on randomized complete block design with four replications, was carried out at the Faculty of Agriculture, Ferdowsi University of Mashhad in 2012. Treatments were temperature ranges (5/15, 10/20, 15/25, 15/30 and 20/35 ° C night / day and different levels of salinity (0, 10, 20, 40, 80, 160 and 320 mM of NaCl. The effect of pH adjusted to 5-9 by using acid buffer solution. Seeds were planted at different depth (0, 1, 2, 3 and 4 cm to study their effects on seedling emergence. Results showed that black henbane exhibited highest germination percentage at 25/15 diurnal tempratures, with 86.50 and 60.50 respectivly for daylight and complete darkness. Germination of ground cherry was highest (93% at 10/20 diurnal temprature and 41% in complete darkness. Highest germination percentage for curly dock at diurnal temperatures of 20/10, 25/15 and 30/20 with light / dark regime of constant darkness was abserved. The seeds at diurnal temprature of 35/25 in both light regimes did not germinate. Increasing salinity reduced germination. Increasing the osmotic potential, also reduced germination significantly. Highest germination percentage of black henbane (97% was observed at pH 7, and lowest germination (17% at pH 9. Increasing planting depth reduced seedling emergence significantly. Germination percentages of ground cherry and curly dock at pH 6 and 7 were highest (97% and 96% respectively and lowest 26 and 25 % respectively at pH 9.

  19. Facile approach to synthesis the curly leaf-like Nano-sheets of g-C3N4 with enhanced photocatalytic ability

    Science.gov (United States)

    Zhao, Zhiren; Li, Kebin; Muhmood, Tahir; Xia, Mingzhu; Wang, Fengyun

    2018-03-01

    Exfoliation of porous g-C3N4 has been proved a very effective way to prepare g-C3N4 nanosheets (2D layered materials). Here, we present an environment-friendly, high-efficiency and easy scale-up preparation method of curly leaf-like g-C3N4 nanosheets (CL-CN) by liquid-phase exfoliation of honeycomb-like porous g-C3N4 (HP-CN). Two-dimensional curly nanosheets have induced excellent physicochemical properties, i.e. large surface area, high fluorescence quantum efficiency, wide band gap and good water-dispersibility. The photocatalytic performance of CL-CN in degradation of RhB under visible light is much better than that of honeycomb-like porous g-C3N4 and bulk g-C3N4. The improved photocatalytic performance of CL-CN is well explained by the improved physicochemical properties and photocatalytic mechanism. In addition, CL-CN being a 2D layered material with excellent photoluminescence characteristic and non-toxic behavior can be widely applied in bio-medicine, bio-imaging and biosensors field.

  20. Joint environmental assessment 1997--2001 of the California Department of Food and Agriculture Curly Top Virus Control Program for Bureau of Land Management and Department of Energy

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The DOE, Naval Petroleum reserves in California (NPRC), proposes to sign an Amendment to the Cooperative Agreement and Supplement with the California Department of Food and Agriculture (CDFA) to extend the term of the Curly Top Virus Control Program (CTVCP) in California. This program involves Malathion spraying on NPRC lands to control the beet leafhopper, over a five year period from 1997 through 2001. It is expected that approximately 330 acres on Naval Petroleum Reserve Number 1 (NPR-1) and approximately 9,603 acres on Naval Petroleum Reserve Number 2 (NPR-2) will be treated with Malathion annually by CDFA during the course of this program. The actual acreage subject to treatment can vary from year to year. Pursuant to the requirements of the National Environmental Policy Act of 1969 (NEPA), as amended, the potential impacts of the proposed action were analyzed in a Joint Environmental Assessment (DOE/EA-1011) with the US Department of Interior, Bureau of Land Management (BLM) acting as lead agency, in consultation with the CDFA, and the DOE acting as a cooperating agency. Based on the analysis in the EA, DOE has determined that the conduct of the Curly Top Virus Control Program in California is not a major Federal action significantly affecting the quality of the human environment, within the meaning of the NEPA. Therefore, the preparation of an Environmental Impact Statement is not required and DOE is consequently issuing a FONSI.

  1. Rare Variant of Ankyloblepharon-ectodermal Defect-cleft Lip/Cleft Palate Syndrome: Curly Hair-ankyloblepharon-nail Disease Syndrome

    Science.gov (United States)

    Chopra, Ajay; Mitra, Debdeep; Kandpal, Renu; Agarwal, Reetu

    2018-01-01

    Ankyloblepharon-ectodermal defect-cleft lip/cleft palate (AEC) syndrome is one of the variants of ectodermal dysplasia. It is an autosomal dominant disorder comprising of ankyloblepharon, ectodermal dysplasia, and cleft palate or cleft lip. In 1976, it wasfirst described by Hay and Wells, therefore also known as Hay–Wells syndrome. The characteristic feature of this syndrome is “ankyloblepharon filiforme adnatum”, which refers to the partial thickness fusion of the eyelid margins. The “curly hair-ankyloblepharon-nail disease (CHAND) syndrome” is a clinical variant of AEC syndrome. We report a rare case of a 7-year-old girl child who presented with history of abnormal dentition, 20 nail dystrophy, and light-colored, sparse curly hairs since birth. Parents gave history that at the time of birth, her both eyelids were fused partially, which was surgically corrected by an ophthalmologist at 1 month of age. There was no history of hypohidrosis or anhidrosis, heat intolerance, cleft lip or cleft palate. Microscopy of the hair shaft found “bubbly hair” morphology. This case is unique as it is a rare presentation, and awareness should be there for this constellation of findings so that the systemic associations can be investigated. “Bubble hair” morphology on microscopy is a unique feature in this rare autosomal recessive condition. PMID:29440852

  2. Presencia de Circulifer tenellus Baker y Beet mild curly top virus en maleza durante el invierno en el centro norte de México Circulifer tenellus Baker and Beet mild curly top virus presence in weeds during the winter in north-central Mexico

    OpenAIRE

    Rodolfo Velásquez-Valle; Luis Roberto Reveles-Torres; Mario Domingo Amador-Ramírez; María Mercedes Medina-Aguilar; Guillermo Medina-García

    2012-01-01

    Una de las enfermedades más importantes del chile para secado en el norte centro de México es la denominada amarillamientos del chile. Existe poca información acerca de la interacción entre el vector (Circulifer tenellus Baker), el Beet mild curly top virus y la maleza durante el invierno en esta región, consecuentemente el objetivo del trabajo fue identificar maleza de invierno que sirve como refugio para el vector y hospedero del virus en esta región. Entre enero y marzo de 2011 se muestrea...

  3. Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses, and metabolism

    Science.gov (United States)

    Background: Escherichia coli O157:H7 (O157) strain 86-24, linked to a 1986 disease outbreak, displays biofilm- and curli-negative phenotypes that are correlated with the lack of Congo red (CR) binding and formation of white colonies (CR negative) on a CR negative containing medium. However, on a CR ...

  4. The Transformative Impact of Undergraduate Research Mentoring on Students and the Role of the Council on Undergraduate Research (CUR) in Supporting Faculty Mentors

    Science.gov (United States)

    Fox, L. K.; Singer, J.

    2015-12-01

    Undergraduate Research (UR) is broadly accepted as a high impact educational practice. Student participation in UR contributes to measurable gains in content knowledge and skills/methodology, oral and written communication skills, problem solving and critical thinking, self-confidence, autonomy, among others. First-generation college students and students from underrepresented minorities that participate in UR are more likely to remain in STEM majors, persist to graduation, and pursue graduate degrees. While engagement in the research process contributes to these outcomes, the impact of the interaction with the faculty mentor is critical. A number of studies provide evidence that it is the relationship that forms with the faculty mentor that is most valued by students and strongly contributes to their career development. Faculty mentors play an important role in student development and the relationship between mentor and student evolves from teacher to coach to colleague. Effective mentoring is not an inherent skill and is generally not taught in graduate school and generally differs from mentoring of graduate students. Each UR mentoring relationship is unique and there are many effective mentoring models and practices documented in the literature. The Council on Undergraduate Research (CUR) has a long history of supporting faculty who engage in research with undergraduates and offers resources for establishing UR programs at individual, departmental, and institutional levels. The Geosciences Division of CUR leads faculty development workshops at professional meetings and provides extensive resources to support geosciences faculty as UR mentors (http://serc.carleton.edu/NAGTWorkshops/undergraduate_research/index.html). Examples of effective mentoring strategies are highlighted, including a model developed by SUNY- Buffalo State that integrates mentoring directly into the evaluation of UR.

  5. Role of curli and plant cultivation conditions on Escherichia coli O157:H7 internalization into spinach grown on hydroponics and in soil.

    Science.gov (United States)

    Macarisin, Dumitru; Patel, Jitendra; Sharma, Vijay K

    2014-03-03

    Contamination of fresh produce could represent a public health concern because no terminal kill step is applied during harvest or at the processing facility to kill pathogens. In addition, once contaminated, pathogens may internalize into produce and be protected from disinfectants during the postharvest processing step. The objective of the current study was to determine the potential internalization of Escherichia coli O157:H7 into spinach roots and subsequent transfer to the edible parts. Because curli are involved in biofilm formation, we investigated whether their presence influence the internalization of E. coli O157:H7 into spinach. Further, the effect of the spinach cultivar on E. coli O157:H7 internalization was evaluated. Spinach plants were grown in contaminated soil as well as hydroponically to prevent mechanical wounding of the roots and inadvertent transfer of pathogens from the contamination source to the non-exposed plant surfaces. Results showed that E. coli O157:H7 could internalize into hydroponically grown intact spinach plants through the root system and move to the stem and leaf level. The incidence of internalization was significantly higher in hydroponically grown plants when roots were exposed to 7 log CFU/mL compared to those exposed to 5 log CFU/mL. The effect of cultivar on E. coli O157:H7 internalization was not significant (P>0.05) for the analyzed spinach varieties, internalization incidences showing almost equal distribution between Space and Waitiki, 49.06% and 50.94% respectively. Wounding of the root system in hydroponically grown spinach increased the incidence of E. coli O157:H7 internalization and translocation to the edible portions of the plant. Experimental contamination of the plants grown in soil resulted in a greater number of internalization events then in those grown hydroponically, suggesting that E. coli O157:H7 internalization is dependent on root damage, which is more likely to occur when plants are grown in soil

  6. Curly arrows meet electron density transfers in chemical reaction mechanisms: from electron localization function (ELF) analysis to valence-shell electron-pair repulsion (VSEPR) inspired interpretation.

    Science.gov (United States)

    Andrés, Juan; Berski, Sławomir; Silvi, Bernard

    2016-07-07

    Probing the electron density transfers during a chemical reaction can provide important insights, making possible to understand and control chemical reactions. This aim has required extensions of the relationships between the traditional chemical concepts and the quantum mechanical ones. The present work examines the detailed chemical insights that have been generated through 100 years of work worldwide on G. N. Lewis's ground breaking paper on The Atom and the Molecule (Lewis, G. N. The Atom and the Molecule, J. Am. Chem. Soc. 1916, 38, 762-785), with a focus on how the determination of reaction mechanisms can be reached applying the bonding evolution theory (BET), emphasizing how curly arrows meet electron density transfers in chemical reaction mechanisms and how the Lewis structure can be recovered. BET that combines the topological analysis of the electron localization function (ELF) and Thom's catastrophe theory (CT) provides a powerful tool providing insight into molecular mechanisms of chemical rearrangements. In agreement with physical laws and quantum theoretical insights, BET can be considered as an appropriate tool to tackle chemical reactivity with a wide range of possible applications. Likewise, the present approach retrieves the classical curly arrows used to describe the rearrangements of chemical bonds for a given reaction mechanism, providing detailed physical grounds for this type of representation. The ideas underlying the valence-shell-electron pair-repulsion (VSEPR) model applied to non-equilibrium geometries provide simple chemical explanations of density transfers. For a given geometry around a central atom, the arrangement of the electronic domain may comply or not with the VSEPR rules according with the valence shell population of the considered atom. A deformation yields arrangements which are either VSEPR defective (at least a domain is missing to match the VSEPR arrangement corresponding to the geometry of the ligands), VSEPR compliant

  7. Evaluation of a synthetic peptide for the detection of anti-Mycobacterium tuberculosis curli pili IgG antibodies in patients with pulmonary tuberculosis.

    Science.gov (United States)

    Naidoo, Natasha; Pillay, Balakrishna; Bubb, Martin; Pym, Alexander; Chiliza, Thamsanqa; Naidoo, Kogieleum; Ndung'u, Thumbi; Kasprowicz, Victoria O; Pillay, Manormoney

    2018-03-01

    Tuberculosis (TB) remains a serious threat in underdeveloped areas. Mycobacterium tuberculosis curli pili (MTP), a virulence factor, is a potential biomarker for a reliable point of care (POC) test and was evaluated for its ability to react with Immunoglobulin G (IgG) in TB patients. An MTP synthetic peptide in a slot blot assay was used to screen serum/plasma samples (n = 65) in 3 separate cohorts, including 40 TB positive (16 HIV co-infected), 20 TB negative/HIV negative patients and 5 healthy volunteers. Forty samples were true positives (HIV positive, n = 16), 23 true negatives (HIV negative) and 2 false positives (HIV negative). The McNemar test demonstrated a 3.08% accuracy estimate (CI: -2.1% - 3.08%). This confirms that MTP is expressed during infection, including HIV-TB co-infection, is likely to be suitable for the design of a POC test and supports the validation of MTP for TB detection in larger patient populations. Copyright © 2018 Elsevier Ltd. All rights reserved.

  8. The Effects on Water Consumption and Some Growth Parameters of Different Level of Leonardite in Curly Leaf Salad (Lactuva sativa var. crispa

    Directory of Open Access Journals (Sweden)

    Sertan Sesveren

    2018-04-01

    Full Text Available In the research, effects of Leonardite which is an important source of humic and fulvic acid applied in different levels on the basis of weight to soil on the water consumption and agricultural productivity in curly leaf salad (Lactuva sativa var. crispa were evaluated. The study was carried out under the greenhouse conditions as a pot experiment. The soil used was clayey-loamy and the PE pots with 10 L volume were used in the experiment. The study was designed to test random blocks with 3 replications in 4 different treatments. The subjects were formed as L0: (Control, L5: (5% leonardite + 95% soil, L10: (10% leonardite + 90% soil and L20: (20% leonardite + 80% soil. Plant water depletions were monitored by weighing the pots. The highest water consumption was achieved with 9.74 L for L0 control (non-leonardite applied potting soil, while it was lowest as 7.20 L for the L20. The effect of the Leonardite applied as different levels on the yield and some growth parameters were found statistically significant (P

  9. cur | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Ethical Guidelines and Procedures document. Posted on 17 January 2017. A revised version of the document 'Scientific Values: Ethical Guidelines and Procedures' has been produced by the Academy's Panel on Scientific Values. For more details, see the Academy Committee on Scientific Values page.

  10. A DEIM Induced CUR Factorization

    Science.gov (United States)

    2015-09-18

    1320866. †Department of Computational and Applied Mathematics , MS 134, Rice University, Houston, Texas 77005-1892. ‡Department of Mathematics , 225...NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Rice University,Department of Computational and Applied Mathematics ...129 0.002 1 1.000 bullet 15 216 35 0.009 38 0.430 music 16 78 3 0.246 24 0.492 south 17 213 19 0.018 110 0.259 their 18 138 14 0.030 43 0.408 please 19

  11. Basic evidence for epidermal H2O2/ONOO(-)-mediated oxidation/nitration in segmental vitiligo is supported by repigmentation of skin and eyelashes after reduction of epidermal H2O2 with topical NB-UVB-activated pseudocatalase PC-KUS.

    Science.gov (United States)

    Schallreuter, Karin U; Salem, Mohammed A E L; Holtz, Sarah; Panske, A

    2013-08-01

    Nonsegmental vitiligo (NSV) is characterized by loss of inherited skin color. The cause of the disease is still unknown despite accumulating in vivo and in vitro evidence of massive epidermal oxidative stress via H2O2 and peroxynitrite (ONOO(-)) in affected individuals. The most favored hypothesis is based on autoimmune mechanisms. Strictly segmental vitiligo (SSV) with dermatomal distribution is a rare entity, often associated with stable outcome. Recently, it was documented that this form can be associated with NSV (mixed vitiligo). We here asked the question whether ROS and possibly ONOO(-) could be players in the pathogenesis of SSV. Our in situ results demonstrate for the first time epidermal biopterin accumulation together with significantly decreased epidermal catalase, thioredoxin/thioreoxin reductase, and MSRA/MSRB expression. Moreover, we show epidermal ONOO(-) accumulation. In vivo FT-Raman spectroscopy reveals the presence of H2O2, methionine sulfoxide, and tryptophan metabolites; i.e., N-formylkynurenine and kynurenine, implying Fenton chemistry in the cascade (n=10). Validation of the basic data stems from successful repigmentation of skin and eyelashes in affected individuals, regardless of SSV or segmental vitiligo in association with NSV after reduction of epidermal H2O2 (n=5). Taken together, our contribution strongly supports H2O2/ONOO-mediated stress in the pathogenesis of SSV. Our findings offer new treatment intervention for lost skin and hair color.

  12. Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses and metabolism.

    Science.gov (United States)

    Sharma, V K; Bayles, D O; Alt, D P; Looft, T; Brunelle, B W; Stasko, J A

    2017-03-08

    Escherichia coli O157:H7 (O157) strain 86-24, linked to a 1986 disease outbreak, displays curli- and biofilm-negative phenotypes that are correlated with the lack of Congo red (CR) binding and formation of white colonies (CR - ) on a CR-containing medium. However, on a CR medium this strain produces red isolates (CR + ) capable of producing curli fimbriae and biofilms. To identify genes controlling differential expression of curli fimbriae and biofilm formation, the RNA-Seq profile of a CR + isolate was compared to the CR - parental isolate. Of the 242 genes expressed differentially in the CR + isolate, 201 genes encoded proteins of known functions while the remaining 41 encoded hypothetical proteins. Among the genes with known functions, 149 were down- and 52 were up-regulated. Some of the upregulated genes were linked to biofilm formation through biosynthesis of curli fimbriae and flagella. The genes encoding transcriptional regulators, such as CsgD, QseB, YkgK, YdeH, Bdm, CspD, BssR and FlhDC, which modulate biofilm formation, were significantly altered in their expression. Several genes of the envelope stress (cpxP), heat shock (rpoH, htpX, degP), oxidative stress (ahpC, katE), nutrient limitation stress (phoB-phoR and pst) response pathways, and amino acid metabolism were downregulated in the CR + isolate. Many genes mediating acid resistance and colanic acid biosynthesis, which influence biofilm formation directly or indirectly, were also down-regulated. Comparative genomics of CR + and CR - isolates revealed the presence of a short duplicated sequence in the rcsB gene of the CR + isolate. The alignment of the amino acid sequences of RcsB of the two isolates showed truncation of RcsB in the CR + isolate at the insertion site of the duplicated sequence. Complementation of CR + isolate with rcsB of the CR - parent restored parental phenotypes to the CR + isolate. The results of this study indicate that RcsB is a global regulator affecting bacterial survival in

  13. Leucemia linfóide aguda: uma doença curável Acute lymphoblastic leukemia: a curable disease

    Directory of Open Access Journals (Sweden)

    Francisco Pedrosa

    2002-04-01

    Full Text Available RESUMO: Leucemia linfóide Aguda (LLA, é o tipo mais comum de câncer infantil, constituindo cerca de um terço de todas as neoplasias malignas da criança. A incidência de LLA em crianças nos Estados Unidos é aproximadamente de 3,4 casos por 100.000 crianças menores de 15 anos de idade. Até a metade do século passado, as leucemias eram consideras universalmene, uma doença fatal. O desenvolvimento de combinações terapêuticas, utilizando diversas drogas citotóxicas com ou sem transplante de medula óssea, tem aumentado o percentual de cura da criança portadora de Leucemia linfóide Aguda em mais de 80%. Anualmente cerca de 1.500 crianças com LLA, nos Estados Unidos, estão sendo curadas. Contudo, dos 75.000 novos casos de LLA, que são diagnosticados anualmente em todo o mundo, cerca de 60.000 (80% não têm acesso a essas modernas formas de tratamento e são, portanto excluídos desse processo de cura. O desafio de tornar a LLA uma doença curável começa a ser vencido com o relato de taxas de sobrevida maior que 50% para crianças tratadas em países com recursos limitados. Em El salvador, Bonilla, conseguiu aumentar a taxa de sobrevida de crianças com LLA de 5% para 50%. No Recife, Pernambuco, Brasil, Pedrosa evoluiu de uma taxa de 29% nos anos 80, para 75% na era atual.ABSTRACT: Acute lymphoblastic leukemia (ALL is the most common type of cancer in children, accoun-ting for one third of all malignant neoplasias affecting them. ALL incidences in children in the United States are of approximately 3,4 cases per 100.000 children under fifteen years old. Up to the middle of last century leukemia was universally considered a fatal di-sease. The development of therapeutic combinations through the use of various cytotoxic drugs with or without bone marrow transplantation has increased cure percentages of children with ALL in over 80%. Annually around 1.500 children with ALL in the Uni-ted States are being cured. Nevertheless, of the

  14. Signal peptide prediction suggests Mycobacterium tuberculosis curli pilin subunit secretion via the Sec pathway may hinder MTP overexpression in Escherichia coli

    International Nuclear Information System (INIS)

    Naidoo, N.; Pillay, B.; Bubb, M.; Kumar, A.; Chiliza, T.; Pillay, M.

    2017-01-01

    Introduction Mycobacterium tuberculosis curli pili (MTP) are novel, potential TB diagnostic biomarkers as they are important virulence attributes, unique to the M. tuberculosis complex (MTBC). The production of high quality recombinant transmembrane and secretory proteins that can serve as biomarkers may be challenging due to their secretion attributes. For example, the signal peptide of MTP governed by the classical secretion pathway may hinder the purification of the protein in E. coli systems. In this study, the secretion characteristics of MTP were determined and the cloning, expression and purification of MTP was attempted in E.coli. Materials and methods A fragment of MTP unique to MTBC was cloned into pet101 and pGEX-6P-1 vectors. The clones were confirmed by nucleotide sequencing and expression of the protein was attempted at IPTG concentrations ranging from 0.1mM to 1mM and at temperatures between 25 °C to 37 °C. The pGEX-6P-1/mtp clone expressed protein was purified, yielding a MTP-GST fusion protein and a free GST band that were analysed by LC/MS mass spectrometry. Inclusion body preparation attempted from the pet101/mtp clone yielded two bands at 10 kDa and below 10 kDa, both of which were analysed by LC/MS mass spectrometry. Transcription activity of both the clones was interrogated by real time PCR on the cDNA derived from the induced clones at various time points after induction with IPTG. The signal peptide and protein secretion characteristics of the MTP protein were determined by bioinformatics analysis of the amino acid sequence using publically available software. Results The truncated MTP fragments were successfully cloned in both the vectors as confirmed by nucleotide sequencing. Expression of the pGEX-6P-1/mtp clone using 0.5 mM IPTG at 27 °C demonstrated the presence of the expected fragment at approximately 35 kDa. This was confirmed by Western Blotting using anti-GST antibodies. However, purification of MTP in adequate quantities as a

  15. Signal peptide prediction suggests Mycobacterium tuberculosis curli pilin subunit secretion via the Sec pathway may hinder MTP overexpression in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Natasha Naidoo

    2017-07-01

    Full Text Available Introduction Mycobacterium tuberculosis curli pili (MTP are novel, potential TB diagnostic biomarkers as they are important virulence attributes, unique to the M. tuberculosis complex (MTBC. The production of high quality recombinant transmembrane and secretory proteins that can serve as biomarkers may be challenging due to their secretion attributes. For example, the signal peptide of MTP governed by the classical secretion pathway may hinder the purification of the protein in E. coli systems. In this study, the secretion characteristics of MTP were determined and the cloning, expression and purification of MTP was attempted in E.coli.  Materials and methods A fragment of MTP unique to MTBC was cloned into pet101 and pGEX-6P-1 vectors. The clones were confirmed by nucleotide sequencing and expression of the protein was attempted at IPTG concentrations ranging from 0.1mM to 1mM and at temperatures between 25 °C to 37 °C. The pGEX-6P-1/mtp clone expressed protein was purified, yielding a MTP-GST fusion protein and a free GST band that were analysed by LC/MS mass spectrometry. Inclusion body preparation attempted from the pet101/mtp clone yielded two bands at 10 kDa and below 10 kDa, both of which were analysed by LC/MS mass spectrometry. Transcription activity of both the clones was interrogated by real time PCR on the cDNA derived from the induced clones at various time points after induction with IPTG. The signal peptide and protein secretion characteristics of the MTP protein were determined by bioinformatics analysis of the amino acid sequence using publically available software.  Results The truncated MTP fragments were successfully cloned in both the vectors as confirmed by nucleotide sequencing. Expression of the pGEX-6P-1/mtp clone using 0.5 mM IPTG at 27 °C demonstrated the presence of the expected fragment at approximately 35 kDa. This was confirmed by Western Blotting using anti-GST antibodies. However, purification of MTP in

  16. Signal peptide prediction suggests Mycobacterium tuberculosis curli pilin subunit secretion via the Sec pathway may hinder MTP overexpression in Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Naidoo, N.; Pillay, B.; Bubb, M.; Kumar, A.; Chiliza, T.; Pillay, M.

    2017-07-01

    Introduction Mycobacterium tuberculosis curli pili (MTP) are novel, potential TB diagnostic biomarkers as they are important virulence attributes, unique to the M. tuberculosis complex (MTBC). The production of high quality recombinant transmembrane and secretory proteins that can serve as biomarkers may be challenging due to their secretion attributes. For example, the signal peptide of MTP governed by the classical secretion pathway may hinder the purification of the protein in E. coli systems. In this study, the secretion characteristics of MTP were determined and the cloning, expression and purification of MTP was attempted in E.coli. Materials and methods A fragment of MTP unique to MTBC was cloned into pet101 and pGEX-6P-1 vectors. The clones were confirmed by nucleotide sequencing and expression of the protein was attempted at IPTG concentrations ranging from 0.1mM to 1mM and at temperatures between 25 °C to 37 °C. The pGEX-6P-1/mtp clone expressed protein was purified, yielding a MTP-GST fusion protein and a free GST band that were analysed by LC/MS mass spectrometry. Inclusion body preparation attempted from the pet101/mtp clone yielded two bands at 10 kDa and below 10 kDa, both of which were analysed by LC/MS mass spectrometry. Transcription activity of both the clones was interrogated by real time PCR on the cDNA derived from the induced clones at various time points after induction with IPTG. The signal peptide and protein secretion characteristics of the MTP protein were determined by bioinformatics analysis of the amino acid sequence using publically available software. Results The truncated MTP fragments were successfully cloned in both the vectors as confirmed by nucleotide sequencing. Expression of the pGEX-6P-1/mtp clone using 0.5 mM IPTG at 27 °C demonstrated the presence of the expected fragment at approximately 35 kDa. This was confirmed by Western Blotting using anti-GST antibodies. However, purification of MTP in adequate quantities as a

  17. Construção do conceito de número: uma análise de atividades matemáticas desenvolvidas pelo Subprojeto PIBID / UFMT / CUR nas escolas do Ensino Fundamental de Rondonópolis

    Directory of Open Access Journals (Sweden)

    Kelly Bomfim Alves de Oliveira

    2015-06-01

    Full Text Available Este artigo origina-se de uma pesquisa fundamentada na teoria de Jean Piaget, realizada no decorrer de atividades do subprojeto Alfabetização Matemática do PIBID / UFMT / CUR / Pedagogia. O objetivo foi investigar e problematizar a importância da construção do conceito de número segundo Piaget no processo de alfabetização matemática. A pesquisa foi qualitativa, com estudo bibliográfico, no qual replicamos 3 provas de conservação numérica, com 2 crianças, sendo 1 de 5 anos e a outra de 6 anos. Os dados foram descritos e analisados utilizando-se o método interpretativo. Consideramos que os resultados nos possibilitou constatar o nível de representação/fase de construção do número que as crianças se encontravam. Palavras-chave: alfabetização matemática; construção do conceito do número; prova de conservação.

  18. C2-mediated decrease in DNA methylation, accumulation of siRNAs, and increase in expression for genes involved in defense pathways in plants infected with beet severe curly top virus.

    Science.gov (United States)

    Yang, Li-Ping; Fang, Yuan-Yuan; An, Chun-Peng; Dong, Li; Zhang, Zhong-Hui; Chen, Hao; Xie, Qi; Guo, Hui-Shan

    2013-03-01

    Cytosine methylation is one of epigenetic information marked on the DNA sequence. In plants, small interfering RNAs (siRNAs) target homologous genomic DNA sequences for cytosine methylation. This process, known as RNA-directed DNA methylation (RdDM), plays an important role in transposon control, regulation of gene expression and virus resistance. In this paper, we demonstrate that the C2 protein encoded by a geminivirus (beet severe curly top virus, BSCTV) mediated a decrease in DNA methylation of repeat regions in the promoters of ACD6, an upstream regulator of the salicylic acid defense pathway, and GSTF14, an endogenous gene of the glutathione S-transferase superfamily that is implicated in numerous stress responses. C2-mediated decreases in DNA methylation reduced accumulation of the siRNAs derived from the promoter repeats and enhanced the steady-state expression of both ACD6 and GSTF14 transcripts. Reduced accumulation of BSCTV-derived siRNAs was detected in BSCTV-infected plants, but not in plants infected with C2-deficient BSCTV (c2(- ) BSCTV). C2 protein exhibited no siRNA-binding activity. Instead, our results revealed that C2 protein-mediated decreases in DNA methylation appeared to affect the production of siRNAs that are required for targeting and reinforcing RdDM, a process that activated expression of defense-related genes that are normally dampened by these siRNAs in the host plants. However, C2-dependent reduction in virus-derived siRNAs also benefits the viruses by disrupting the feedback loop reinforcing DNA methylation-mediated antiviral silencing. © 2012 The Authors The Plant Journal © 2012 Blackwell Publishing Ltd.

  19. Methodology for Project Governence in CURS

    OpenAIRE

    Hribovšek, Anja

    2010-01-01

    Rare are organizations that haven’t met with project way of work yet. This also applies to government bodies. The degree addresses two areas which are very important for organizations that want to maintain control over costs, risk, functionality and complexity of a project. The first area is project management and the second area is system development process. The main purpose of the degree is to execute a complete analysis on both mentioned areas and to prepare or to renovate the met...

  20. Disseny, implementació i avaluació d'un curs de formació on-line per a mestres : "Recursos i aplicació de les TIC a l'educació infantil i primària"

    OpenAIRE

    Calvo Gordillo, Rosa Mª

    2014-01-01

    En aquest treball final de màster s'ha dissenyat una proposta de formació on-line per a mestres d'educació infantil i primària sobre el coneixement i la utilització d'algunes eines TIC de la web 2.0 a l'àmbit educatiu. Aquest curs de formació ha sigut desenvolupat al CEFIRE de Torrent, en València. En este trabajo final de máster se ha diseñado una propuesta de formación on-line para maestros de educación infantil y primaria sobre el conocimiento y la utilización de algunas herramientas TI...

  1. High-temperature ceramic-curly building insulation ВЫСОКОТЕМПЕРАТУРНАЯ КЕРАМОВОЛОКНИСТАЯ ТЕПЛИЗОЛЯЦИЯ

    Directory of Open Access Journals (Sweden)

    Treskova Nadezhda Vladimirovna

    2011-07-01

    Full Text Available The technology of composite fiber products for high-temperature insulation and the properties research is described. The ceramic-curly materials application for line operating of thermal generating units is justified.ТЕПЛИЗОЛЯЦИЯ Рассмотрена технология композиционных волокнистых изделий для высокотемпературной изоляции и исследованы их свойства. Обосновано применение керамоволокнистых материалов при футеровочных работах тепловых агрегатов.

  2. Cur Wild Neighbors: Teaching Unit (Grades 1-3).

    Science.gov (United States)

    Sammut-Tovar, Dorothy

    Designed to sensitize primary grade students to the responsibilities of protecting wild animals, this teaching unit contains a variety of interdisciplinary worksheets and activities. Although focusing on wild animals indigenous to San Mateo County (California), materials are easily adaptable for use in other areas. Examples of activities are…

  3. Statutul Curții Penale Internaționale

    Directory of Open Access Journals (Sweden)

    Daniel BUDA

    2002-02-01

    Full Text Available International Penal Court is an international juridical and permanent institution with general and unique competition in trying and penalisation of psihical persons acused by serious infringements of the international humanitary law. The international penalisation of genocide infrictional, crimes against humanity, war crimes and the agresion crimes constitute an imperative and an obligation of international comunity.

  4. EL CUR DEBETUR: ¿ELEMENTO ESENCIAL DEL CONTRATO?

    Directory of Open Access Journals (Sweden)

    Arsul José Vázquez Pérez

    2008-11-01

    Full Text Available Desde una perspectiva integral se valoran las principales proposiciones teóricas que en torno a la causa contractual han sido realizadas por los creadores de las más importantes concepciones en torno al tema. Se comprenden los períodos desde el Derecho Romano hasta la última teoría que sobre la causa de los contratos ha tenido relevancia en la aplicación normativa y jurisprudencial. Con un nuevo enfoque del problema, el autor alcanza un resultado que riñe con las antiguas posiciones y esboza los postulados de una nueva concepción en torno a la causa en este tipo de negocios jurídicos. Si bien es una posición crítica a las concepciones imperantes en torno al tema, el criterio del autor aprovecha lo mejor de las concepciones precedentes y contribuye en cierta medida a perfeccionar la noción de causa en el derecho de contratos.

  5. Rectal Prolapse Associated with Constipation in a Curly Colt (Case ...

    African Journals Online (AJOL)

    However, it must be differentiated from prolapsed ileo-ceco colic intussusceptions by passing a probe, blunt instrument, or finger between the prolapsed mass and the inner rectal wall. In rectal prolapse, the instrument cannot be inserted due to the presence of a fornix Cynthia, (2005), this is very important in differentiating ...

  6. Atmospheric NH3 deposition, S and N metabolism in curly kale

    NARCIS (Netherlands)

    Ambrosio de Castro, Ana Helena

    2006-01-01

    A well-regulated nutrient uptake is of crucial importance for every organism. Plants, as sessile organisms have developed different strategies to cope with environmental changes. Therefore, the uptake of nitrogen and sulfur are regulated in a coordinate manner and adjusted to meet the nutrient

  7. Food Science and Technology Abstracts: guia ràpida [text]. Curs 2017-18

    OpenAIRE

    Universitat de Barcelona. CRAI

    2017-01-01

    Guia ràpida de la base de dades Food Science and Technology Abstracts (FSTA) que és una base de dades bibliogràfica d'àmbit mundial sobre ciència, tecnologia i química alimentària, nutrició i salut humana, biotecnologia i toxicologia.

  8. Food Science and Technology Abstracts: guia ràpida [text]. Curs 2015-16

    OpenAIRE

    Universitat de Barcelona. CRAI

    2016-01-01

    Guia ràpida de la base de dades Food Science and Technology Abstracts (FSTA) que és una base de dades bibliogràfica d'àmbit mundial sobre ciència, tecnologia i química alimentària, nutrició i salut humana, biotecnologia i toxicologia.

  9. Web of Science [vídeo]: tutorial. Curs 2016-17

    OpenAIRE

    Universitat de Barcelona. CRAI

    2016-01-01

    El portal Web of Science (WOS), abans conegut com a Web of Knowledge, ofereix un conjunt de recursos bibliogràfics. A través del WOS es poden fer cerques transversals a les bases de dades que l'integren: Biosis Previews, CAB Abstracts, Zoological Record i també Medline.

  10. CINAHL: guia ràpida [text]. Curs 2016-17

    OpenAIRE

    Universitat de Barcelona. CRAI

    2016-01-01

    CINAHL (Cumulative Index to Nursing and Allied Health Literature) és una base de dades que conté informació bibliogràfica de publicacions especialitzades en infermeria, biomedicina, medicina alternativa i altres disciplines relacionades amb ciències de la salut.

  11. CINAHL COMPLETE: guia d'ús [text]. Curs 2016-17

    OpenAIRE

    Universitat de Barcelona. CRAI

    2017-01-01

    CINAHL COMPLETE (Cumulative Index to Nursing and Allied Health Literature) és una base de dades que conté informació bibliogràfica de publicacions especialitzades en infermeria, biomedicina, medicina alternativa i altres disciplines relacionades amb ciències de la salut.

  12. THE INFLUENCE OF CROSS-TRAINING DUE TO SPA TREATMENT IN CURRING FUNCTIONAL NEUROTIC DISORDERS

    Directory of Open Access Journals (Sweden)

    Rade Stefanović

    2006-06-01

    Full Text Available Modern civilization moves to endless automation and mechanization. This fact, in one hand, has positive effect on increase of life standard, but also carries its own risks and threats of mankind. Axelberg, famous academician, makes an interesting remark that only a hundred years ago man did 96 % of his work by himself, while today most manufacturing processes are 100% automated. Stress, lack of physical activity and bad food regime are canses of organic damages of cardiopulmonary system. Latest research, conducted in many countries, shows that for proper functioning of every organism the minimum of moving is necessary.Term neurosis was introduced in medical literature by Scottish doctor W. Cullen in 1777. By that term were considered different clinical appearances which are believed neurotic todey, but also the phenomena today considered as psychosomatic disorders of character and behavior. Pavlov’s reflexive theory is basic in treatment of some types of neurossis’ during spa treatments in Nerečinska Banja. It usually starts at the beginning of May and ends in October, but sometimes if weather is nice, it can last until the middle of November. The treatment cure last tventy-one day, doesn’t include medicaments, but only spa water, baths and massage.

  13. Pràctiques de tècniques instrumentals separatives (Curs 2012/2013)

    OpenAIRE

    Maicas i Prieto, Sergi

    2013-01-01

    El quadernet forma part dels materials docents programats mitjançant l'ajut del Servei de Política Lingüística de la Universitat de València. Cromatografia. La cromatografia en paper és una tècnica analítica que s’utilitza per a la separació i la identificació de mescles que són, o poden ser, de color. Aquest mètode ha estat substituït en gran mesura per la cromatografia de capa fina. Tanmateix, continua sent una poderosa eina per l’ensenyament. En algunes ocasions es realitzen cromatograf...

  14. Com cooperen els estudiants de primer curs de ADE? Treball en equip: disseny basat en Economia Experimental

    Directory of Open Access Journals (Sweden)

    Penélope Hernández

    2013-06-01

    Full Text Available En este trabajo presentamos el diseño y los resultados de un experimento económico realizado por estudiantes de primero del grado de administración y dirección de empresas en el transcurso de una clase práctica de la asignatura transversal de incorporación a los estudios universitarios. Los estudiantes tomaron sus decisiones en un marco donde coexisten los conflictos individuales con los colectivos. El efecto del grupo les ilustró el efecto de la coordinación y del trabajo en equipo. El principal objetivo de esta actividad es desarrollar y evaluar la competencia de trabajo en equipo, así como la responsabilidad social, capacidad reflexiva y actitud crítica de los estudiantes.

  15. Why God Became Man: Saint Anselm, Cur Deus Homo, and a Religious Challenge in Anglo-Norman England

    National Research Council Canada - National Science Library

    Wolfe, Adam

    2004-01-01

    ... of Canterbury during the Gregorian Reform. Anselm's life was theology in action, and yet no historian has fully explored the possibility that Anselm's policies as head of the Catholic Church in England were the natural outgrowth...

  16. Straight or Curly? Hair and Race in Carmen Montañez's "Pelo bueno, pelo malo"

    Science.gov (United States)

    Ortiz-Loyola, Brenda

    2017-01-01

    Historically, black women's hair has been a site where power and social relations are defined. In Puerto Rico, cultural production has been critical in perpetuating as well as in contesting the prevailing white European ideal of beauty and its impact on women's hairstyling practices. Nevertheless, the link between aesthetic preferences and the…

  17. Hha Represses Biofilm Formation in Escherichia coli O157:H7 by Affecting the Expression of Flagella and Curli Fimbriae

    Science.gov (United States)

    Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen that produces a broad-spectrum of diarrheal illnesses in infected humans. Although the genetic and molecular mechanisms enabling EHEC O157:H7 to produce characteristic adherence on epithelial cells are well characterized, the g...

  18. Effect of H2S exposure on S-35-sulfate uptake, transport and utilization in curly kale

    NARCIS (Netherlands)

    Westerman, S; Weidner, W; De Kok, LJ; Stulen, I.

    2000-01-01

    When Brassica oleracea L. was exposed to 0.2 mul l(-1) H2S the sulfate uptake measured during a dark or light period was decreased to the same extent. Both the xylem loading and the net sulfate uptake rate were decreased by 42% after 6 days of exposure to 0.4 mul l(-1) H2S. This suggested that the

  19. Phthiriasis palpebrarum misdiagnosed as allergic ...

    African Journals Online (AJOL)

    Removal of the eyelashes, cutting of scalp hairs, and phenothrin shampoo may be effective in treating phthiriasis palpebrarum. In cases of blepharoconjunctivitis, eyelids and eyelashes should be carefully examined by slit lamp to avoid misdiagnosis. Key words: Blepharoconjunctivitis, crab louse, eyelashes, nits, pthirus ...

  20. Cornelia de Lange syndrome with optic disk pit: Novel association and review of literature.

    Science.gov (United States)

    Shenoy, Bhamy Hariprasad; Gupta, Amit; Sachdeva, Virender; Kekunnaya, Ramesh

    2014-05-01

    Cornelia de Lange syndrome (CdLS), also called Brachmann-de Lange syndrome, is a multiple congenital anomaly syndrome characterized by a distinctive facial appearance, ophthalmological abnormalities, prenatal and postnatal growth deficiency, psychomotor delay, behavioral problems, and malformations of the upper extremities. Most common and consistent ophthalmic features reported are nasolacrimal duct obstruction, long and curly eyelashes, blepharitis, ptosis, synophrys, telecanthus, hypertelorism, microcornea, peripapillary pigment ring, and myopia. In this report we report a case of a 5-year old boy who presented to our institution with complaint of blurring of vision in the right eye since birth. A diagnosis of Cornelia de Lange syndrome was arrived at based on the characteristic external and ophthalmic examination. He was found to have a rare association of optic nerve head coloboma in the right eye and a novel finding of an optic disk pit in the left eye. The association of optic disk pit with CdLS has never been reported earlier. We aim to provide a thorough review of literature of this not so uncommon syndrome.

  1. Cornelia de Lange syndrome with optic disk pit: Novel association and review of literature

    Directory of Open Access Journals (Sweden)

    Bhamy Hariprasad Shenoy

    2014-01-01

    Full Text Available Cornelia de Lange syndrome (CdLS, also called Brachmann-de Lange syndrome, is a multiple congenital anomaly syndrome characterized by a distinctive facial appearance, ophthalmological abnormalities, prenatal and postnatal growth deficiency, psychomotor delay, behavioral problems, and malformations of the upper extremities. Most common and consistent ophthalmic features reported are nasolacrimal duct obstruction, long and curly eyelashes, blepharitis, ptosis, synophrys, telecanthus, hypertelorism, microcornea, peripapillary pigment ring, and myopia. In this report we report a case of a 5-year old boy who presented to our institution with complaint of blurring of vision in the right eye since birth. A diagnosis of Cornelia de Lange syndrome was arrived at based on the characteristic external and ophthalmic examination. He was found to have a rare association of optic nerve head coloboma in the right eye and a novel finding of an optic disk pit in the left eye. The association of optic disk pit with CdLS has never been reported earlier. We aim to provide a thorough review of literature of this not so uncommon syndrome.

  2. Distribution and Abundance of Eurasian Watermilfoil (Myriophyllum spicatum L.) and Curly-Leaf Pondweed (Potamogeton crispus L.) in Shawano Lake, Wisconsin

    National Research Council Canada - National Science Library

    Owens, Chetta S; James, William F; Skogerboe, John G; Smart, R. M

    2007-01-01

    ... (Potamogeton crispus L.) and Eurasian watermilfoil (Myriophyllum spicatum L.). In addition to problems associated with invasive plant species, Shawano Lake has been experiencing declining water quality associated with phosphorus (P...

  3. Blowout heroes : while his crew mate risked fire and falling rock to save him, Curly Slater fought right to the last breath

    Energy Technology Data Exchange (ETDEWEB)

    Louie, J.

    2008-11-15

    This article discussed a blow-out that resulted in a fatality at a drilling rig near Helmut, British Columbia (BC). The blowout caused a column of fire, drilling mud, and rock to explode from the floor of the rig. As the rig's derrickman was trapped when drilling mud caked onto his easy rider cable and froze the escape sliding mechanism. The derrickman fell and was left dangling by his harness. Crew members spotted him from a distance and returned to the site in order to unhook the easy rider cable from the manifold shack and move the steel line to a location that would allow him to descend more easily. The derrickman was unable to un-jam his fall arrestor. He freed himself using his own strength, seized a cable, but lost his grip while still 75 feet in the air and fell to his death. The tragedy was caused by the presence of a very large gas pocket encountered at an unusually shallow depth. Several of the crew members have been unable to continue working in the oil and gas industry, due to the psychological trauma of witnessing the man's death. The Canadian Association of Oilwell Drilling Contractors (CAODC) has issued awards of merit for the crew men who attempted to save the derrickman, and the entire crew will receive medals of bravery from the Governor General of Canada. 2 figs.

  4. Corpusurile paralele. O modalitate de cercetare în timp real a schimbării lingvistice în curs de realizare

    Directory of Open Access Journals (Sweden)

    Christian Mair

    2015-01-01

    Full Text Available Lucrarea valorifică date din patru corpusuri corespondente de cîte un milion de cuvinte, Brown (Engleză americană, 1961, LOB (Engleză britanică, 1961, Frown (Engleză americană, 1992 și FLOB (Engleză britanică, 1991 pentru a oferi o descriere integrată a variației sincronice (regionale și stilistice și a schimbărilor diacronice pe termen scurt din engleza literară scrisă. Analiza unui număr relativ mare de variabile morfosintactice arată că schimbările structurale directe sînt mai degrabă rare în perioada avută în vedere. Cu toate acestea, există numeroase evoluții diacronice semnificative sub aspect statistic care, considerate împreună, oferă dovezi ce atestă o tendință discursiv-pragmatică unitară, și anume, „colocvializarea” normelor limbii engleze scrise. Se consideră că această evoluție lingvistică este motivată de un curent socio-cultural mai larg, deplasarea gustului public către o exprimare informală din ce în ce mai pregnantă.

  5. Role of curli and contamination level on Escherichia coli O157:H7 internalization into organic spinach plants grown on hydroponics and in soil

    Science.gov (United States)

    Introduction: E. coli O157:H7 may be internalized into organic leafy greens via root uptake. Understanding the mechanisms of E. coli O157:H7 internalization into organic leafy greens is important as produce wash treatment may not remove internalized pathogens. Purpose: The internalization potential...

  6. Role of curli and plant cultivation conditions on Escherichia coli O157:H7 internalization into organic spinach grown on hydroponics and in soil

    Science.gov (United States)

    Contamination of organic fresh produce is an important public health concern because no terminal disinfection treatment is applied during harvest or at the packing facility to kill pathogens. In addition, once contaminated, pathogens may internalize into produce and be protected from disinfectants d...

  7. Mascara induced milphosis, an etiological evaluation.

    Science.gov (United States)

    Kadri, Rajani; Achar, Asha; Tantry, Thrivikrama Padur; Parameshwar, Devika; Kudva, Ajay; Hegde, Sudhir

    2013-07-01

    Eyelashes play an important role in one's personality and builds confidence. Now-a- days, mascaras are used very commonly as an eye cosmetic. Mascara induced loss of eyelashes has been evaluated in this study. The aim of this study is to determine the effect of mascara on eyelashes and to find an association between the usage of mascara and fall of eyelashes. Cross-sectional questionnaire based study done on medical students. Medical students were interviewed with a semi-structured questionnaire on mascara usage. MedCalc 9.3.6.0 version. A total of 128 medical students who used mascara were included in this study. Mean (standard deviation) age of the students was 23.7 ± 2.0 years. Nearly 31% of the subjects faced the eye problems. Eyelash fall was observed in 19%. Higher mean years of use of mascara (5.17 ± 3.8 vs. 3.19 ± 2.6, P = 0.027, t-test) influenced the fall of eyelashes in the subjects. Itching of the eye prior to fall of eyelashes was observed in all subjects (P = 0.0002, Fisher exact test). A higher percentage of eyelash falls was observed in subjects who used water for removal of waterproof mascara (27%). There is a significant positive association between long-term use of eye cosmetics like mascara and fall of eyelashes. Furthermore, use of water for removal of waterproof mascara was associated with a higher incidence of eyelash fall.

  8. ORIGINAL ARTICLE

    African Journals Online (AJOL)

    User

    trend all over the world. Long eyelashes are consid- ... cara to thicken the look of eyelashes which creates a bold look (Champion, ... natural look. To the beautician, the procedure may seem simple. However, from the health care point of view, it is still important to pay close attention to its effect on vision and how safe the ...

  9. Metabolic fate of poly-(lactic-co-glycolic acid-based curcumin nanoparticles following oral administration

    Directory of Open Access Journals (Sweden)

    Harigae T

    2016-06-01

    Full Text Available Takahiro Harigae,1 Kiyotaka Nakagawa,1 Taiki Miyazawa,2 Nao Inoue,3 Fumiko Kimura,1 Ikuo Ikeda,3 Teruo Miyazawa4,5 1Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; 2Vascular Biology Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA; 3Laboratory of Food and Biomolecular Science, Graduate School of Agricultural Science, 4Food and Biotechnology Innovation Project, New Industry Creation Hatchery Center, 5Food and Health Science Research Unit, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan Purpose: Curcumin (CUR, the main polyphenol in turmeric, is poorly absorbed and rapidly metabolized following oral administration, which severely curtails its bioavailability. Poly-(lactic-co-glycolic acid-based CUR nanoparticles (CUR-NP have recently been suggested to improve CUR bioavailability, but this has not been fully verified. Specifically, no data are available about curcumin glucuronide (CURG, the major metabolite of CUR found in the plasma following oral administration of CUR-NP. Herein, we investigated the absorption and metabolism of CUR-NP and evaluated whether CUR-NP improves CUR bioavailability.Methods: Following oral administration of CUR-NP in rats, we analyzed the plasma and organ distribution of CUR and its metabolites using high-performance liquid chromatography-tandem mass spectrometry. To elucidate the mechanism of increased intestinal absorption of CUR-NP, we prepared mixed micelles comprised of phosphatidylcholine and bile salts and examined the micellar solubility of CUR-NP. Additionally, we investigated the cellular incorporation of the resultant micelles into differentiated Caco-2 human intestinal cells.Results: Following in vivo administration of CUR-NP, CUR was effectively absorbed and present mainly as CURG in the plasma which contained significant amounts of the metabolite compared with

  10. High drug payload curcumin nanosuspensions stabilized by mPEG-DSPE and SPC: in vitro and in vivo evaluation.

    Science.gov (United States)

    Hong, Jingyi; Liu, Yingying; Xiao, Yao; Yang, Xiaofeng; Su, Wenjing; Zhang, Mingzhu; Liao, Yonghong; Kuang, Haixue; Wang, Xiangtao

    2017-11-01

    Curcumin (CUR) is a promising drug candidate based on its broad bioactivities and good antitumor effect, but the application of CUR is potentially restricted because of its poor solubility and bioavailability. This study aims at developing a simple and effective drug delivery system for CUR to enhance its solubility and bioavailability thus to improve its antitumor efficacy. Curcumin nanosuspensions (CUR-NSps) were prepared by precipitation-ultrasonication method using mPEG2000-DSPE and soybean lecithin as a combined stabilizer. CUR-NSps with a high drug payload of 67.07% were successfully prepared. The resultant CUR-NSps had a mean particle size of 186.33 ± 2.73 nm with a zeta potential of -19.00 ± 1.31 mV. In vitro cytotoxicity assay showed that CUR-NSps exhibited enhanced cytotoxicity compared to CUR solution. The pharmacokinetics results demonstrated that CUR-NSps exhibited a significantly greater AUC 0-24 and prolonged MRT compared to CUR injections after intravenous administration. In the biodistribution study, CUR-NSps demonstrated enhanced biodistribution compared with CUR injections in liver, spleen, kidney, brain, and tumor. The CUR-NSps also showed improved antitumor therapeutic efficacy over the injections (70.34% versus 40.03%, p < 0.01). These results suggest that CUR-NSps might represent a promising drug formulation for intravenous administration of CUR for the treatment of cancer.

  11. Hybridization of polyvinylpyrrolidone to a binary composite of curcumin/α-glucosyl stevia improves both oral absorption and photochemical stability of curcumin.

    Science.gov (United States)

    Kadota, Kazunori; Okamoto, Daiki; Sato, Hideyuki; Onoue, Satomi; Otsu, Shigeyuki; Tozuka, Yuichi

    2016-12-15

    The tri-component system curcumin/α-glucosyl stevia (Stevia-G)/polyvinylpyrrolidone (PVP) was developed to improve the oral bioavailability and physicochemical properties of curcumin (CUR). The tri-component CUR formulation with Stevia-G and PVP was prepared with freeze-drying. The tri-component CUR system exhibited 13,000-fold higher solubility of CUR than the equilibrium solubility of CUR for 24h, indicating a stable tri-composite structure involving CUR. CUR could be converted into an amorphous form in the presence of Stevia-G and PVP by freeze-drying. The photo-degradation of CUR in the tri-component system was negligible even under an amorphous state of CUR. After oral administration in rats, the oral absorption of the tri-component CUR formulation (20mgCUR/kg) was 6.7-fold higher than that of crystalline CUR. The tri-component CUR formulation would therefore be a promising option to improve physicochemical properties and oral absorption of CUR. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Influence of clay minerals on curcumin properties: Stability and singlet oxygen generation

    Science.gov (United States)

    Gonçalves, Joyce L. S.; Valandro, Silvano R.; Poli, Alessandra L.; Schmitt, Carla C.

    2017-09-01

    Curcumin (CUR) has showed promising photophysical properties regarding to biological and chemical sciences. However, the main barrier for those applications are their low solubility and stability in aqueous solution. The effects of two different clay minerals, the montmorillonite (SWy-2) and the Laponite RD (Lap) nanoclay, on the stabilization of Curcumin were investigated. Their effects were compared with two well-established environments (acidic and neutral aqueous media). CUR/clay hybrids were prepared using a simple and fast method, where CUR solution was added into clay suspensions, to obtain well dispersed hybrids in water. The degradation process of CUR and CUR/clays hybrids was investigated using UV-Vis spectroscopic. For both studied hybrids, the CUR degradation process was suppressed by the presence of the clay particles. Furthermore, the Lap showed a great stabilization effect than SWy-2. This behavior was due to the smaller particle size and higher exfoliation ability of Lap, providing a large surface for CUR adsorption compared to SWy-2. The degradation process of CUR solutions and CUR/clay hybrids was also studied in the presence of light. CUR photodegradation process was faster not only in the aqueous solution but also in the clay suspension compared to those studied in the dark. The presence of clay particles accelerated the photodegradation of CUR due to the products formation in the reactions between CUR and oxygen radicals. Our results showed that the singlet oxygen quantum yield (ΦΔ) of CUR were about 59% higher in the clay suspensions than CUR in aqueous solution. Therefore, the formation of CUR/clay hybrids, in particularly with Lap, suppressed the degradation in absence light of CUR and increased the singlet oxygen generation, which makes this hybrids of CUR/clay a promising material to enlarge the application of CUR in the biological sciences.

  13. Interactions of cationic trimeric, gemini and monomeric surfactants with trianionic curcumin in aqueous solution.

    Science.gov (United States)

    Wang, Meina; Wu, Chunxian; Tang, Yongqiang; Fan, Yaxun; Han, Yuchun; Wang, Yilin

    2014-05-21

    Interactions of trianionic curcumin (Cur(3-)) with a series of cationic surfactants, monomeric surfactant dodecyl trimethylammonium bromide (DTAB), dimeric surfactant hexamethylene-1,6-bis(dodecyldimethylammonium bromide) (12-6-12) and trimeric surfactant tri(dodecyldimethylammonioacetoxy)diethyltriamine trichloride (DTAD), have been investigated in aqueous solution of pH 13.0. Surface tension and spectral measurements indicate that the cationic surfactants display a similar surfactant concentration dependent interaction process with Cur(3-), involving three interaction stages. At first the three cationic surfactants electrostatically bind on Cur(3-) to form the surfactant-Cur(3-) complex. Then the bound and unbound cationic surfactants with Cur(3-) aggregate into surfactant-Cur(3-) mixed micelles through hydrophobic interactions above the critical micelle concentration of the surfactants (CMCC) in the presence of Cur(3-). Finally excess unbound surfactants self-assemble into micelles like those without Cur(3-). For all the three surfactants, the addition of Cur(3-) only decreases the critical micelle concentration of 12-6-12 but does not affect the critical micelle concentration of DTAB and DTAD. As the oligomeric degree of surfactants increases, the intermolecular interaction of the cationic surfactants with Cur(3-) increases and the surfactant amount needed for Cur(3-) encapsulation decreases. Compared with 12-6-12, either the weaker interaction of DTAB with Cur(3-) or stronger interaction of DTAD with Cur(3-) limits the stability or solubility of Cur(3-) in surfactant micelles. Therefore, gemini surfactant 12-6-12 is the best choice to effectively suppress Cur(3-) degradation at very low concentrations. Isothermal titration microcalorimetry, surface tension and (1)H NMR results reveal that 12-6-12 and Cur(3-) form a (12-6-12)2-Cur(3-) complex and start to form micelles at extremely decreased concentrations, where either 12-6-12 or Cur(3-) works as a bridge

  14. Iris and periocular adverse reactions to bimatoprost in Japanese patients with glaucoma or ocular hypertension

    Directory of Open Access Journals (Sweden)

    Inoue K

    2012-01-01

    Full Text Available Kenji Inoue1, Minako Shiokawa1, Michitaka Sugahara1, Risako Higa1, Masato Wakakura1, Goji Tomita21Inouye Eye Hospital, Tokyo, Japan; 2Second Department of Ophthalmology, Toho University School of Medicine, Tokyo, JapanPurpose: To prospectively investigate adverse reactions to bimatoprost in Japanese patients with glaucoma or ocular hypertension. We also examined patient attitudes to adverse reactions via a questionnaire.Methods: Fifty-two Japanese patients with glaucoma or ocular hypertension were enrolled. Iridial, eyelid, and eyelash photographs were taken before and at 6 months after bimatoprost treatment. Increase in eyelid pigmentation, iridial pigmentation, eyelash growth and bristle, and vellus hair of the lid was assessed from the photographs. Questionnaires completed by patients provided insight into their subjective judgment of adverse reactions.Results: Increase in eyelash bristle (53.8%, iris pigmentation (50.0%, eyelash growth (46.2%, vellus hair of the lid (40.4%, and eyelid pigmentation (7.7% was evident after bimatoprost treatment. The objective and subjective assessments were in agreement in terms of increase in eyelash bristle, eyelash growth, and increase in vellus hair of the lid.Conclusion: Most patients were conscious of these adverse reactions. Before administering bimatoprost, sufficient explanation of potential adverse reactions should be provided; after initiating treatment, careful observation is required.Keywords: bimatoprost, adverse reaction, eyelid pigmentation, changes in eyelashes, iris pigmentation 

  15. Curcumin loaded NLC induces histone hypoacetylation in the CNS after intraperitoneal administration in mice.

    Science.gov (United States)

    Puglia, Carmelo; Frasca, Giuseppina; Musumeci, Teresa; Rizza, Luisa; Puglisi, Giovanni; Bonina, Francesco; Chiechio, Santina

    2012-06-01

    The natural p300-specific histone acetyltransferase (HAT) inhibitor, curcumin (CUR), has been widely investigated for its potential therapeutic effect as an anticancer and anti-inflammatory agent. Notwithstanding this interesting pharmacological profile, CUR shows some drawbacks, such as poor absorption and a very fast metabolism and elimination, that limit its clinical use. Aim of the present study was to formulate CUR loaded nanostructured lipid carriers (NLC-CUR) in order to improve the bioavailability and stability of this compound after systemic administration with increased effects in the central nervous system (CNS). NLC-CUR were prepared and characterized on their physicochemical properties by PCS and DSC analyses. Thus, NLC-CUR were systemically injected and the effects in the CNS were compared with a CUR control formulation containing 0.05% DMSO (DMSO-CUR). Our results demonstrate that CUR is able to decrease histone acetylation in the CNS when included in NLCs. Western blot analysis shows that intraperitoneal injection of NLC-CUR (100mg/kg) in mice induces a marked hypoacetylation of histone 4 (H4) at lysine 12 (K12) in the spinal cord compared with control group. Notably, DMSO-CUR (100mg/kg) did not change the H4K12 acetylation level in the CNS. Our study suggests a novel approach to ameliorate the pharmacokinetics of CUR that allows a better permeation in the CNS. Copyright © 2012 Elsevier B.V. All rights reserved.

  16. Curcumin complexation with cyclodextrins by the autoclave process: Method development and characterization of complex formation.

    Science.gov (United States)

    Hagbani, Turki Al; Nazzal, Sami

    2017-03-30

    One approach to enhance curcumin (CUR) aqueous solubility is to use cyclodextrins (CDs) to form inclusion complexes where CUR is encapsulated as a guest molecule within the internal cavity of the water-soluble CD. Several methods have been reported for the complexation of CUR with CDs. Limited information, however, is available on the use of the autoclave process (AU) in complex formation. The aims of this work were therefore to (1) investigate and evaluate the AU cycle as a complex formation method to enhance CUR solubility; (2) compare the efficacy of the AU process with the freeze-drying (FD) and evaporation (EV) processes in complex formation; and (3) confirm CUR stability by characterizing CUR:CD complexes by NMR, Raman spectroscopy, DSC, and XRD. Significant differences were found in the saturation solubility of CUR from its complexes with CD when prepared by the three complexation methods. The AU yielded a complex with expected chemical and physical fingerprints for a CUR:CD inclusion complex that maintained the chemical integrity and stability of CUR and provided the highest solubility of CUR in water. Physical and chemical characterizations of the AU complexes confirmed the encapsulated of CUR inside the CD cavity and the transformation of the crystalline CUR:CD inclusion complex to an amorphous form. It was concluded that the autoclave process with its short processing time could be used as an alternate and efficient methods for drug:CD complexation. Copyright © 2017 Elsevier B.V. All rights reserved.

  17. Trachoma

    Science.gov (United States)

    ... very irritated, the eyelashes may turn in and rub against the cornea. This can cause corneal ulcers, ... Pathogenesis and control of blinding trachoma. In: Duane TD, Tasman W, Jaeger EA, eds. Duane's Ophthalmology 2013 . ...

  18. Disease: H01905 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available tions, such as allergic rhinitis, atopic dermatitis, HIV infection, and certain e...also acquired conditions and drugs that are known to cause eyelash trichomegaly. For example, acquired condi

  19. Sty

    Science.gov (United States)

    ... that causes inflammation of the eyelid is a chalazion. A chalazion occurs when there's a blockage in one of ... just behind the eyelashes. Unlike a sty, a chalazion usually isn't painful and tends to be ...

  20. Lice

    Science.gov (United States)

    ... your pubic area and, less frequently, on coarse body hair, such as chest hair, eyebrows or eyelashes. You ... your scalp, body, clothing, or pubic or other body hair. Adult lice may be about the size of ...

  1. Effect of novel curcumin-encapsulated chitosan-bioglass drug on bone and skin repair after gamma radiation: experimental study on a Wistar rat model.

    Science.gov (United States)

    Jebahi, S; Saoudi, M; Farhat, L; Oudadesse, H; Rebai, T; Kabir, A; El Feki, A; Keskes, H

    2015-04-01

    Radiation therapy contributes to a significant increase in bone osteoporosis and skin loss. Various natural health products might be beneficial to reduce bone and skin alterations. Curcumin (CUR) medicines derived from natural plants have played an important role in health care. This study aims at synthesizing and evaluating the performance therapy of CUR-encapsulated bioglass-chitosan (CUR-BG-CH). In vitro, the antioxidant assay was evaluated by using 1,1-diphenyl-2-picrylhydrazyl free-radical (DPPH) scavenging and the nitroblue tetrazolium reduction. The CUR-BG-CH antimicrobial effects were tested in liquid media. In vivo, after rat (60) Co γ-radiation, the tissue wound-healing process was studied by grafting CUR and CUR-BG-CH in femoral condyle and dorsal skin rat tissue. The antioxidant studies indicated that CUR-BG-CH quenches free radicals more efficiently than unmodified CUR and had effective DPPH (91%) and superoxide anion (51%) radical scavenging activities. The CUR-BG-CH biomaterial exhibited an important antimicrobial activity against Staphylococcus aureus. The histomorphometric parameters showed amelioration in CUR-BG-CH-treated rats. An improved mechanical property was noticed (33.16 ± 5.0 HV) when compared with that of unmodified CUR group (23.15 ± 4.9 HV). A significant decrease in tumour necrosis factor-α cytokine production was noted in the CUR-BG-CH rats (90 pg/ml) as compared with that of unmodified CUR group (240 pg/ml). The total amount of hydroxyproline was significantly enhanced (33.5%) in CUR-BG-CH group as compared with that of control. Our findings suggested that CUR-BG-CH might have promising potential applications for wound healing. Copyright © 2015 John Wiley & Sons, Ltd.

  2. Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications.

    Science.gov (United States)

    Yallapu, Murali M; Othman, Shadi F; Curtis, Evan T; Bauer, Nichole A; Chauhan, Neeraj; Kumar, Deepak; Jaggi, Meena; Chauhan, Subhash C

    2012-01-01

    The next generation magnetic nanoparticles (MNPs) with theranostic applications have attracted significant attention and will greatly improve nanomedicine in cancer therapeutics. Such novel MNP formulations must have ultra-low particle size, high inherent magnetic properties, effective imaging, drug targeting, and drug delivery properties. To achieve these characteristic properties, a curcumin-loaded MNP (MNP-CUR) formulation was developed. MNPs were prepared by chemical precipitation method and loaded with curcumin (CUR) using diffusion method. The physicochemical properties of MNP-CUR were characterized using dynamic light scattering, transmission electron microscopy, and spectroscopy. The internalization of MNP-CUR was achieved after 6 hours incubation with MDA-MB-231 breast cancer cells. The anticancer potential was evaluated by a tetrazolium-based dye and colony formation assays. Further, to prove MNP-CUR results in superior therapeutic effects over CUR, the mitochondrial membrane potential integrity and reactive oxygen species generation were determined. Magnetic resonance imaging capability and magnetic targeting property were also evaluated. MNP-CUR exhibited individual particle grain size of ~9 nm and hydrodynamic average aggregative particle size of ~123 nm. Internalized MNP-CUR showed a preferential uptake in MDA-MB-231 cells in a concentration-dependent manner and demonstrated accumulation throughout the cell, which indicates that particles are not attached on the cell surface but internalized through endocytosis. MNP-CUR displayed strong anticancer properties compared to free CUR. MNP-CUR also amplified loss of potential integrity and generation of reactive oxygen species upon treatment compared to free CUR. Furthermore, MNP-CUR exhibited superior magnetic resonance imaging characteristics and significantly increased the targeting capability of CUR. MNP-CUR exhibits potent anticancer activity along with imaging and magnetic targeting capabilities. This

  3. Clinical management of trichotillomania with bimatoprost.

    Science.gov (United States)

    Peabody, Todd; Reitz, Sarah; Smith, Jedidiah; Teti, Brittany

    2013-06-01

    This case report presents the use of bimatoprost 0.03% to help regrow eyelashes on a patient suffering from trichotillomania. Trichotillomania is characterized by repetitive compulsive hair pulling that leads to noticeable hair loss. Trichotillomania sufferers are affected by this condition in many ways including shame, embarrassment, guilt, and lower self-esteem. Although a formal therapy regimen does not exist, current treatment approaches include behavioral therapy and pharmacotherapy. In cases where a patient pulls the eyelashes, topical bimatoprost (Latisse) may be used to treat madarosis of the eyelashes by increasing growth, including length, thickness, and darkness. A 55-year-old white female presented to the clinic with an interest in using bimatoprost ophthalmic solution (Latisse; Allergan). The patient reported a history of eyelash pulling since age 12 and began treatment to control the impulses with amitriptyline 1 week before her appointment. Follow-up appointments showed increased lash growth bilaterally over a 4-month period as a result of Latisse usage. Those who pull their eyelashes may benefit from using bimatoprost 0.03%. The management of trichotillomania involving the eyelashes presents an opportunity for eye care professionals to collaborate with mental health providers for the betterment of shared patients. By expediting the patient's lash growth, Latisse can improve patients' self-image, reinforce compliance, and play an important adjunctive role in any therapy regimen.

  4. Particle size effect of curcumin nanosuspensions on cytotoxicity, cellular internalization, in vivo pharmacokinetics and biodistribution.

    Science.gov (United States)

    Bi, Chao; Miao, Xiao Qing; Chow, Sing Fung; Wu, Wen Jin; Yan, Ru; Liao, Y H; Chow, Albert Hee-Lum; Zheng, Ying

    2017-04-01

    This study aimed to investigate the particle size effect on bioactivity, cellular internalization and bioavailability of curcumin (CUR) nanosuspension (CUR-NS). CUR-NSs of different particle sizes were prepared by two different anti-solvent precipitation methods. CUR-NS with the smallest size showed similar in vitro anticancer activity and bioavailability to the CUR solution, whereas nanosuspensions of larger particle sizes displayed higher in vitro cellular internalization and cytotoxicity, as well as higher in vivo AUC and slower clearance rate after i.v. administration in rats. CUR solution and different sized CUR-NSs reached the highest concentrations in the lung, followed by liver and spleen while the lowest concentration was observed in the brain after i.v. administration in mice. Specifically, CUR-NS of 70nm accumulated more in the brain, whereas CUR-NS of 200nm accrued more in liver and spleen. CUR-NS of 20nm displayed no significant biodistribution difference compared with CUR solution in all tissues. Copyright © 2016 Elsevier Inc. All rights reserved.

  5. Visible Light-Cured Glycol Chitosan Hydrogel Containing a Beta-Cyclodextrin-Curcumin Inclusion Complex Improves Wound Healing In Vivo.

    Science.gov (United States)

    Yoon, Sun-Jung; Hyun, Hoon; Lee, Deok-Won; Yang, Dae Hyeok

    2017-09-10

    Scarless wound healing is ideal for patients suffering from soft tissue defects. In this study, we prepared a novel wet dressing (β-CD-ic-CUR/GC) based on the visible light-cured glycol chitosan (GC) hydrogel and inclusion complex between beta-cyclodextrin (β-CD) and curcumin (CUR). We also evaluated its efficacy in the acceleration of wound healing as compared to that of CUR-loaded GC (CUR/GC). The conjugation of glycidyl methacrylate (GM) to GC for photo-curing was confirmed by ¹H-NMR measurement, and the photo-cured GC hydrogel was characterized by the analyses of rheology, swelling ratio, SEM and degradation rate. After visible light irradiation, the surface/cross-sectional morphologies and storage (G')/loss (G'') moduli revealed the formation of hydrogel with interconnected porosity. The dressing β-CD-ic-CUR/GC exhibited a controlled release of 90% CUR in a sustained manner for 30 days. On the other hand, CUR/GC showed CUR release of 16%. β-CD acted as an excipient in improving the water-solubility of CUR and affected the release behavior of CUR. The in vivo animal tests including measurement of the remaining unhealed wound area and histological analyses showed that β-CD-ic-CUR/GC may have potential as a wet dressing agent to enhance soft tissue recovery in open fractures.

  6. Enhanced photocytotoxicity of curcumin delivered by solid lipid nanoparticles

    Directory of Open Access Journals (Sweden)

    Jiang S

    2016-12-01

    Full Text Available Shan Jiang,1 Rongrong Zhu,1 Xiaolie He,1 Jiao Wang,1 Mei Wang,1 Yechang Qian,2 Shilong Wang1 1Tenth People’s Hospital, School of Life Science and Technology, Tongji University, 2Department of Respiratory Disease, Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, People’s Republic of China Abstract: Curcumin (Cur is a promising photosensitizer that could be used in photodynamic therapy. However, its poor solubility and hydrolytic instability limit its clinical use. The aim of the present study was to encapsulate Cur into solid lipid nanoparticles (SLNs in order to improve its therapeutic activity. The Cur-loaded SLNs (Cur-SLNs were prepared using an emulsification and low-temperature solidification method. The functions of Cur and Cur-SLNs were studied on the non-small cell lung cancer A549 cells for photodynamic therapy. The results revealed that Cur-SLNs induced ~2.27-fold toxicity higher than free Cur at a low concentration of 15 µM under light excitation, stocking more cell cycle at G2/M phase. Cur-SLNs could act as an efficient drug delivery system to increase the intracellular concentration of Cur and its accumulation in mitochondria; meanwhile, the hydrolytic stability of free Cur could be improved. Furthermore, Cur-SLNs exposed to 430 nm light could produce more reactive oxygen species to induce the disruption of mitochondrial membrane potential. Western blot analysis revealed that Cur-SLNs increased the expression of caspase-3, caspase-9 proteins and promoted the ratio of Bax/Bcl-2. Overall, the results from these studies demonstrated that the SLNs could enhance the phototoxic effects of Cur. Keywords: photodynamic therapy, curcumin, solid lipid nanoparticles, drug delivery, reactive oxygen species

  7. Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae.

    Science.gov (United States)

    Azad, Gajendra Kumar; Singh, Vikash; Thakare, Mayur Jankiram; Baranwal, Shivani; Tomar, Raghuvir Singh

    2014-12-19

    Curcumin (CUR), an active polyphenol derived from the spice turmeric, has been traditionally used for centuries in ancient Indian medicine to treat a number of diseases. The physiological effects of CUR have been shown to be diverse; however, the target molecules and pathways that CUR affects have yet to be fully described. Here, we demonstrate for the first time that the budding yeast mitogen-activated protein kinase (MAPK) Hog1 is essential for the response to CUR. Moreover, CUR-induced Hog1 phosphorylation was rescued by supplementation of iron to the growth medium. Hog1 was rapidly phosphorylated upon CUR treatment, but unlike the response to hyperosmotic shock (0.8 M NaCl), it remains activated for an extended period of time. A detailed analysis of HOG pathway mutants revealed that Pbs2p, Ptc2p, and Ssk2p are required for optimal CUR-induced Hog1 phosphorylation. We also observed a Hog1 dependent transcriptional response to CUR treatment that involved the up-regulation of glycerol-3-phosphate dehydrogenase 1 (GPD1), a factor that is essential for the hyperosmotic stress response. Our present finding revealed the role of Hog1 MAPK in regulation of CUR-induced transcriptional response. We anticipate that our finding will enhance the understanding on the molecular mode of action of CUR on S. cerevisiae.

  8. Biodegradable micelles enhance the antiglioma activity of curcumin in vitro and in vivo

    Directory of Open Access Journals (Sweden)

    Zheng S

    2016-06-01

    Full Text Available Songping Zheng,1,* Xiang Gao,1,2,* Xiaoxiao Liu,1 Ting Yu,1 Tianying Zheng,1 Yi Wang,1 Chao You1 1Department of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, People’s Republic of China; 2Department of Pharmacology, Yale School of Medicine, Yale University, New Haven, CT, USA *These authors contributed equally to this work Abstract: Curcumin (Cur, a natural polyphenol of Curcuma longa, has been recently reported to possess antitumor activities. However, due to its poor aqueous solubility and low biological availability, the clinical application of Cur is quite limited. The encapsulation of hydrophobic drugs into nanoparticles is an effective way to improve their pharmaceutical activities. In this research, nanomicelles loaded with Cur were formulated by a self-assembly method with biodegradable monomethoxy poly(ethylene glycol-poly(lactide copolymers (MPEG-PLAs. After encapsulation, the cellular uptake was increased and Cur could be released from MPEG-PLA micelles in a sustained manner. The Cur-loaded MPEG-PLA micelles (Cur/MPEG-PLA micelles exhibited an enhanced toxicity on C6 and U251 glioma cells and induced more apoptosis on C6 glioma cells compared with free Cur. Moreover, the therapy efficiency of Cur/MPEG-PLA micelles was evaluated at length on a nude mouse model bearing glioma. The Cur/MPEG-PLA micelles were more effective on suppressing tumor growth compared with free Cur, which indicated that Cur/MPEG-PLA micelles improved the antiglioma activity of Cur in vivo. The results of immunohistochemical and immunofluorescent analysis indicated that the induction of apoptosis, antiangiogenesis, and inhibition of cell proliferation may contribute to the improvement in antiglioma effects. Our data suggested that Cur/MPEG-PLA may have potential clinic applications in glioma therapy. Keywords: curcumin, glioma, cell apoptosis, cell proliferation, angiogenesis 

  9. Transformation of curcumin from food additive to multifunctional medicine: nanotechnology bridging the gap.

    Science.gov (United States)

    Ahmad, Mohammad Zaki; Akhter, Shabib; Mohsin, Nehal; Abdel-Wahab, Basel A; Ahmad, Javed; Warsi, Musarrat Husain; Rahman, Mahfoozur; Mallick, Neha; Ahmad, Farhan Jalees

    2014-01-01

    Curcumin (CUR) is a yellow-coloured polyphenolic compound obtained from the rhizomes of Curcuma longa. In-depth pharmacological screening of curcumin has given the evidence that CUR persuades shielding and curative effects against various cancers, cardiovascular, wound healing effect and neuro disorders etc owning to anti-oxidant, antiproliferative, anti-inflammatory, anti-angiogenic and antimicrobial activities. However, miserable bioavailability due to poor aqueous solubility limits the application of CUR in various ailments. Different methodologies including the nanoparticle technology have been reported for the bioavailability enhancement of CUR. Nanoparticles exhibit not only the improvement in the solubility of CUR and alike lipophilic molecules (resulted in improved bioavailability) but also giving the opportunity for the disease specific cellular and organ targeting. Improved bioavailability and disease based site specific delivery of CUR is more likely to bring it as a safe multifunctional medicine.

  10. Curcumin Nanoparticle Therapy for Gulf War Illness

    Science.gov (United States)

    2017-10-01

    and stress. It is envisaged that improved cognitive, memory and mood function in GWI-rats with nCUR administration will occur via improvements in...biodegradable polymer nanosystems (nCUR) for alleviating cognitive, memory and mood impairments in a rat model of gulf war illness (GWI). Specific...Aim 1 studies involve quantification of the efficacy of oral administration of different doses of nCUR for improving neurogenesis and suppressing

  11. Novel star-type methoxy-poly(ethylene glycol) (PEG)-poly({epsilon}-caprolactone) (PCL) copolymeric nanoparticles for controlled release of curcumin

    Energy Technology Data Exchange (ETDEWEB)

    Feng Runliang; Zhu Wenxia; Song Zhimei, E-mail: zhimei_song@126.com [University of Jinan, Shandong Academy of Medical Science, Department of Pharmaceutical Engineering, School of Medicine and Life Sciences (China); Zhao Liyan [Hebei North University, Department of Pharmacy (China); Zhai Guangxi [Shandong University, Department of Pharmaceutics, College of Pharmacy (China)

    2013-06-15

    To improve curcumin's (CURs) water solubility and release property, a novel star methoxy poly(ethylene glycol)-poly({epsilon}-caprolactone) (MPEG-PCL) copolymer was synthesized through O-alkylation, basic hydrolysis and ring-opening polymerization reaction with MPEG, epichlorohydrin, and {epsilon}-caprolactone as raw materials. The structure of the novel copolymer was characterized by {sup 1}H NMR, FT-IR, and GPC. The results of FT-IR and differential scanning calorimeter of CUR-loaded nanoparticles (NPs) prepared by dialysis method showed that CUR was successfully encapsulated into the SMP12 copolymeric NPs with 98.2 % of entrapment efficiency, 10.91 % of drug loading, and 88.4 {+-} 11.2 nm of mean particle diameter in amorphous forms. The dissolubility of nanoparticulate CUR was increased by 1.38 Multiplication-Sign 10{sup 5} times over CUR in water. The obtained blank copolymer showed no hemolysis. A sustained CUR release to a total of approximately 56.13 % was discovered from CUR-NPs in 40 % of ethanol saline solution within 72 h on the use of dialysis method. The release behavior fitted the ambiexponent and biphasic kinetics equation. In conclusion, the copolymeric NPs loading CUR might serve as a potential nanocarrier to improve the solubility and release property of CUR.

  12. Bovine Serum Albumin (BSA) coated iron oxide magnetic nanoparticles as biocompatible carriers for curcumin-anticancer drug.

    Science.gov (United States)

    Nosrati, Hamed; Sefidi, Naser; Sharafi, Ali; Danafar, Hossein; Kheiri Manjili, Hamidreza

    2018-02-01

    The bovine serum albumin-coated magnetic nanoparticles (F@BSA NPs) were prepared as curcumin (CUR) carriers through desolvation and chemical co-precipitation process. The characteristics of CUR loaded F@BSA NPs (F@BSA@CUR NPs) were determined by X-ray diffraction (XRD), thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and vibrating-sampling magnetometry (VSM) techniques. It was found that the synthesized F@BSA@CUR NPs were spherical in shape with an average size of 56 ± 11.43 nm (mean ± SD (n = 33)), ζ-potential of -10.1 mV, and good magnetic responsivity. Meanwhile, the drug content of the nanoparticles was 6.88%. These F@BSA@CUR NPs also demonstrated sustained release of CUR at 37 °C in different buffer solutions. Cellular toxicity of F@BSA@CUR NPs was studied on HFF2 cell line. Also, the cytotoxicity of F@BSA@CUR NPs towards MCF-7 breast cancer cells was investigated. The results revealed that F@BSA@CUR NPs have significant cytotoxicity activity on MCF-7 cell line. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Cancer Therapy Using Ultrahigh Hydrophobic Drug-Loaded Graphene Derivatives

    Science.gov (United States)

    Some, Surajit; Gwon, A-Ryeong; Hwang, Eunhee; Bahn, Ga-hee; Yoon, Yeoheung; Kim, Youngmin; Kim, Seol-Hee; Bak, Sora; Yang, Junghee; Jo, Dong-Gyu; Lee, Hyoyoung

    2014-01-01

    This study aimed to demonstrate that curcumin (Cur)-containing graphene composites have high anticancer activity. Specifically, graphene-derivatives were used as nanovectors for the delivery of the hydrophobic anticancer drug Cur based on pH dependence. Different Cur-graphene composites were prepared based on polar interactions between Cur and the number of oxygen-containing functional groups of respective starting materials. The degree of drug-loading was found to be increased by increasing the number of oxygen-containing functional groups in graphene-derivatives. We demonstrated a synergistic effect of Cur-graphene composites on cancer cell death (HCT 116) both in vitro and in vivo. As-prepared graphene quantum dot (GQD)-Cur composites contained the highest amount of Cur nano-particles and exhibited the best anticancer activity compared to the other composites including Cur alone at the same dose. This is the first example of synergistic chemotherapy using GQD-Cur composites simultaneous with superficial bioprobes for tumor imaging. PMID:25204358

  14. Novel star-type methoxy-poly(ethylene glycol) (PEG)–poly(ε-caprolactone) (PCL) copolymeric nanoparticles for controlled release of curcumin

    International Nuclear Information System (INIS)

    Feng Runliang; Zhu Wenxia; Song Zhimei; Zhao Liyan; Zhai Guangxi

    2013-01-01

    To improve curcumin’s (CURs) water solubility and release property, a novel star methoxy poly(ethylene glycol)–poly(ε-caprolactone) (MPEG–PCL) copolymer was synthesized through O-alkylation, basic hydrolysis and ring-opening polymerization reaction with MPEG, epichlorohydrin, and ε-caprolactone as raw materials. The structure of the novel copolymer was characterized by 1 H NMR, FT-IR, and GPC. The results of FT-IR and differential scanning calorimeter of CUR-loaded nanoparticles (NPs) prepared by dialysis method showed that CUR was successfully encapsulated into the SMP12 copolymeric NPs with 98.2 % of entrapment efficiency, 10.91 % of drug loading, and 88.4 ± 11.2 nm of mean particle diameter in amorphous forms. The dissolubility of nanoparticulate CUR was increased by 1.38 × 10 5 times over CUR in water. The obtained blank copolymer showed no hemolysis. A sustained CUR release to a total of approximately 56.13 % was discovered from CUR-NPs in 40 % of ethanol saline solution within 72 h on the use of dialysis method. The release behavior fitted the ambiexponent and biphasic kinetics equation. In conclusion, the copolymeric NPs loading CUR might serve as a potential nanocarrier to improve the solubility and release property of CUR.

  15. Novel star-type methoxy-poly(ethylene glycol) (PEG)-poly(ɛ-caprolactone) (PCL) copolymeric nanoparticles for controlled release of curcumin

    Science.gov (United States)

    Feng, Runliang; Zhu, Wenxia; Song, Zhimei; Zhao, Liyan; Zhai, Guangxi

    2013-06-01

    To improve curcumin's (CURs) water solubility and release property, a novel star methoxy poly(ethylene glycol)-poly(ɛ-caprolactone) (MPEG-PCL) copolymer was synthesized through O-alkylation, basic hydrolysis and ring-opening polymerization reaction with MPEG, epichlorohydrin, and ɛ-caprolactone as raw materials. The structure of the novel copolymer was characterized by 1H NMR, FT-IR, and GPC. The results of FT-IR and differential scanning calorimeter of CUR-loaded nanoparticles (NPs) prepared by dialysis method showed that CUR was successfully encapsulated into the SMP12 copolymeric NPs with 98.2 % of entrapment efficiency, 10.91 % of drug loading, and 88.4 ± 11.2 nm of mean particle diameter in amorphous forms. The dissolubility of nanoparticulate CUR was increased by 1.38 × 105 times over CUR in water. The obtained blank copolymer showed no hemolysis. A sustained CUR release to a total of approximately 56.13 % was discovered from CUR-NPs in 40 % of ethanol saline solution within 72 h on the use of dialysis method. The release behavior fitted the ambiexponent and biphasic kinetics equation. In conclusion, the copolymeric NPs loading CUR might serve as a potential nanocarrier to improve the solubility and release property of CUR.

  16. Enhanced Bioavailability and Anticancer Effect of Curcumin-Loaded Electrospun Nanofiber: In Vitro and In Vivo Study

    Science.gov (United States)

    Wang, Chuan; Ma, Chao; Wu, Zhenkai; Liang, He; Yan, Peng; Song, Jia; Ma, Nan; Zhao, Qinghua

    2015-11-01

    Nanofibers have attracted increasing attention in drug delivery and other biomedical applications due to their some special properties. The present study aims to prepare a fiber-based nanosolid dispersion system to enhance the bioavailability of curcumin (CUR). CUR-loaded polyvinyl pyrrolidone (CUR@PVP) nanofibers were successfully prepared via electrospinning. Scanning electron microscopy (SEM) was employed to observe the morphology of the nanofibers, and the SEM image showed that the drug-loaded nanofibers were smooth, and no CUR clusters were found on the surface of the nanofibers. The results of X-ray diffraction (XRD) demonstrated that the CUR was evenly distributed in the nanofibers in an amorphous state. Fourier transform infrared (FTIR) spectroscopy analysis indicated that intermolecular hydrogen bonding occurred between the CUR and the polymer matrix. In vitro dissolution profiles showed that CUR@PVP nanofiber could be quickly dissolved in phosphate-buffered saline (PBS) solution, while negligible dissolution was observed in pure CUR sample. Importantly, in vitro cell viability assays and in vivo animal tests revealed that the nanosolid dispersion system dramatically enhanced the bioavailability and showed effective anticancer effect of the CUR.

  17. Functionalization of nanodiamond with vitamin E TPGS to facilitate oral absorption of curcumin.

    Science.gov (United States)

    Cheng, Bingchao; Pan, Hao; Liu, Dandan; Li, Dongyang; Li, Jinyu; Yu, Shihui; Tan, Guoxin; Pan, Weisan

    2018-04-05

    The purpose of this work was to develop a d-α-tocopherol polyethylene glycol 1000 succinate (TPGS) decorated nanodiamond (ND) system loading water-insoluble curcumin (ND/CUR/TPGS) to improve the colloidal dispersity and oral bioavailability of the preparation. CUR was physically loaded into ND clusters, then TPGS was coated to the ND/CUR complex forming amorphous nanostructure on the interparticle nanocage of the ND substrate. The formulation of the nanocomplexes was optimized using response surface methodology, and the optimal ND/CUR/TPGS showed small particle size (196.32 nm), high drug loading efficiency (81.59%) and core-shell structure. In vitro release study demonstrated that the nanocomplexes provided a sustained release behavior. The absorptive concentration of ND/CUR/TPGS was dramatically improved in total intestinal tract compared with CUR suspension, and the absorption was controlled by multiple transcytosis mechanisms. Furthermore, the pharmacokinetic studies demonstrated that ND/CUR/TPGS had significantly higher C max (4.50-fold), larger AUC 0-t (10.67-fold), and longer MRT 0-t (3.07-fold) in contrast with that of CUR suspension. Therefore, ND/CUR/TPGS presented great potential for oral delivery of insoluble and poorly permeable drugs. Copyright © 2018 Elsevier B.V. All rights reserved.

  18. CDIS: Circle Density Based Iris Segmentation

    Science.gov (United States)

    Gupta, Anand; Kumari, Anita; Kundu, Boris; Agarwal, Isha

    Biometrics is an automated approach of measuring and analysing physical and behavioural characteristics for identity verification. The stability of the Iris texture makes it a robust biometric tool for security and authentication purposes. Reliable Segmentation of Iris is a necessary precondition as an error at this stage will propagate into later stages and requires proper segmentation of non-ideal images having noises like eyelashes, etc. Iris Segmentation work has been done earlier but we feel it lacks in detecting iris in low contrast images, removal of specular reflections, eyelids and eyelashes. Hence, it motivates us to enhance the said parameters. Thus, we advocate a new approach CDIS for Iris segmentation along with new algorithms for removal of eyelashes, eyelids and specular reflections and pupil segmentation. The results obtained have been presented using GAR vs. FAR graphs at the end and have been compared with prior works related to segmentation of iris.

  19. Inhibitory Activity Of Curcumin Derivatives Towards Metal-free And Metal-induced Amyloid-β Aggregation.

    Science.gov (United States)

    Kochi, Akiko; Lee, Hyuck Jin; Vithanarachchi, Sashiprabha M; Padmini, Vediappen; Allen, Matthew J; Lim, Mi Hee

    2015-01-01

    When Alzheimer's disease (AD) progresses, several pathological features arise including accumulation of misfolded protein aggregates [e.g., amyloid-β (Aβ) plaques], metal ion dyshomeostasis, and oxidative stress. These characteristics are recently suggested to be interconnected through a potential factor, metal-associated Aβ (metal-Aβ) species. The role of metal-Aβ species in AD pathogenesis remains unclear, however. To elucidate the contribution of metal-Aβ species to AD pathology, as well as to develop small molecules as chemical tools and/or theranostic (therapeutic and diagnostic) agents for this disease, curcumin (Cur), a natural product from turmeric, and its derivatives have been studied towards both metal-free and metal-induced Aβ aggregation. Although Cur has indicated anti-amyloidogenic activities and antioxidant properties, its biological use has been hindered due to low solubility and stability in physiologically relevant conditions. Herein, we report the reactivity of Cur and its derivatives (Gd-Cur, a potential multimodal Aβ imaging agent; Cur-S, a water soluble derivative of Cur that has substitution at the phenolic hydroxyls) with metal-free Aβ and metal-Aβ species. Our results and observations indicate that Gd-Cur could modulate Cu(II)-triggered Aβ aggregation more noticeably over metal-free or Zn(II)-induced analogues; however, Cur-S was not observed to noticeably modulate Aβ aggregation with and without metal ions. Overall, our studies present information that could aid in optimizing the molecular scaffold of Cur for the development of chemical tools or theranostics for metal-Aβ species.

  20. Curcumin Protects against Monosodium Glutamate Neurotoxicity and Decreasing NMDA2B and mGluR5 Expression in Rat Hippocampus

    Directory of Open Access Journals (Sweden)

    Rania M. Khalil

    2016-08-01

    Full Text Available Background: Monosodium glutamate (MSG is a flavor enhancer used in food industries. MSG is well documented to induce neurotoxicity. Curcumin (CUR reportedly possesses beneficial effects against various neurotoxic insults. Hence, this present study has been designed to evaluate the neuroprotective effect of curcumin on MSG-induced neurotoxicity in rats. Methods: Thirty-two male Wister rats were divided into four groups (n=8: Control group, MSG group, CUR group and MSG + CUR group. CUR (Curcumin 150 mg/kg, orally was given day after day for four weeks along with MSG (4 mg/kg, orally. After 4 weeks, rats were sacrificed and brain hippocampus was isolated immediately on ice. Inflammatory marker TNFα and acetylcholinesterase (AChE activity (marker for cholinergic function were estimated. Gene expressions of metabotropic glutamate receptor 5 (mGluR5 and N-methyl-D-aspartate receptor 2B (NMDA2B along with glutamate concentration were assessed. Results: Treatment with CUR significantly attenuated AChE activity and TNFα in MSG-treated animals. The anti-inflammatory properties of CUR may be responsible for this observed neuroprotective action. A possible role of CUR to attenuate both glutamate level and gene expression of NMDA2B and mGLUR5 in brain hippocampus was established when compared to MSG group. Conclusion: We concluded that CUR as flavor enhancer protects against MSG-induced neurotoxicity in rats.

  1. Novel Dual Mitochondrial and CD44 Receptor Targeting Nanoparticles for Redox Stimuli-Triggered Release

    Science.gov (United States)

    Wang, Kaili; Qi, Mengjiao; Guo, Chunjing; Yu, Yueming; Wang, Bingjie; Fang, Lei; Liu, Mengna; Wang, Zhen; Fan, Xinxin; Chen, Daquan

    2018-02-01

    In this work, novel mitochondrial and CD44 receptor dual-targeting redox-sensitive multifunctional nanoparticles (micelles) based on oligomeric hyaluronic acid (oHA) were proposed. The amphiphilic nanocarrier was prepared by (5-carboxypentyl)triphenylphosphonium bromide (TPP), oligomeric hyaluronic acid (oHA), disulfide bond, and curcumin (Cur), named as TPP-oHA-S-S-Cur. The TPP targeted the mitochondria, the antitumor drug Cur served as a hydrophobic core, the CD44 receptor targeting oHA worked as a hydrophilic shell, and the disulfide bond acted as a connecting arm. The chemical structure of TPP-oHA-S-S-Cur was characterized by 1HNMR technology. Cur was loaded into the TPP-oHA-S-S-Cur micelles by self-assembly. Some properties, including the preparation of micelles, morphology, redox sensitivity, and mitochondrial targeting, were studied. The results showed that TPP-oHA-S-S-Cur micelles had a mean diameter of 122.4 ± 23.4 nm, zeta potential - 26.55 ± 4.99 mV. In vitro release study and cellular uptake test showed that TPP-oHA-S-S-Cur micelles had redox sensibility, dual targeting to mitochondrial and CD44 receptor. This work provided a promising smart multifunctional nanocarrier platform to enhance the solubility, decrease the side effects, and improve the therapeutic efficacy of anticancer drugs.

  2. Telomerase: a target for therapeutic effects of curcumin and a curcumin derivative in Aβ1-42 insult in vitro.

    Science.gov (United States)

    Xiao, Zijian; Zhang, Aiwu; Lin, Jianwen; Zheng, Zhenyang; Shi, Xiaolei; Di, Wei; Qi, Weiwei; Zhu, Yingting; Zhou, Guijuan; Fang, Yannan

    2014-01-01

    This study was designed to investigate whether telomerase was involved in the neuroprotective effect of curcumin and Cur1. Alzheimer's disease is a consequence of an imbalance between the generation and clearance of amyloid-beta peptide in the brain. In this study, we used Aβ1-42 (10 µg/ml) to establish a damaged cell model, and curcumin and Cur1 were used in treatment groups. We measured cell survival and cell growth, intracellular oxidative stress and hTERT expression. After RNA interference, the effects of curcumin and Cur1 on cells were verified. Exposure to Aβ1-42 resulted in significant oxidative stress and cell toxicity, and the expression of hTERT was significantly decreased. Curcumin and Cur1 both protected SK-N-SH cells from Aβ1-42 and up-regulated the expression of hTERT. Furthermore, Cur1 demonstrated stronger protective effects than curcumin. However, when telomerase was inhibited by TERT siRNA, the neuroprotection by curcumin and Cur1 were ceased. Our study indicated that the neuroprotective effects of curcumin and Cur1 depend on telomerase, and thus telomerase may be a target for therapeutic effects of curcumin and Cur1.

  3. Download this PDF file

    African Journals Online (AJOL)

    Sensitization of singlet oxygen. RESULTS AND DISCUSSION. Figure 2 shows the emission and absorption spectra of CUR and Th. The emission spectrum of CUR (normalised at 520 nm) overlaps with the absorption spectrum of Th with the overlap integral of 4.7 x 10'13 (equation ( 1). 2.50. 2.00. Absotbanee. 1.00. 0.50 ".

  4. Evaluation of an oral carrier system in rats: bioavailability and gastrointestinal absorption properties of curcumin encapsulated PBCA nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Sun Min; Zhao Lixia; Guo Chenyu; Cao Fengliang; Chen Huanlei; Zhao Liyan; Tan Qi; Zhu Xiuqing; Zhu Fanping; Ding Tingting; Zhai Yingjie; Zhai Guangxi, E-mail: professorzhai@yeah.net [Shandong University, Department of Pharmaceutics, College of Pharmacy (China)

    2012-02-15

    A new oral delivery system, polybutylcyanoacrylate nanoparticles (PBCNs), was introduced to improve the oral bioavailability of curcumin (CUR), a poorly soluble drug. The formulation was optimized by orthogonal design and the optimal PBCNs loading CUR exhibited a spherical shape under transmission electron microscopy with a range of 40-400 nm. Physicochemical state of CUR in PBCN was investigated by X-ray diffraction and the possible structure changes occurring in CUR after conjugating with polybutylcyanoacrylate were studied with FTIR. The results indicated that CUR in PBCN was in a non-crystalline state and CUR was encapsulated in PBCN without chemical reaction. The oral pharmacokinetic study was conducted in rats and the relative bioavailability of CUR encapsulated PBCNs to the crude CUR was more than 800%. The in situ absorption experiment in rat intestine indicated the absorption was first order with passive diffusion mechanism. The absorption results in various segments of intestine showed that the main absorption sites were ileum and colon. It can be concluded that PBCNs as an oral carrier can significantly improve the oral absorption of a poorly soluble drug.

  5. Reliability of quay walls using finite element analysiscalibration of partial safety factors in quay wall design by probabilistic plaxis calculations

    NARCIS (Netherlands)

    Wolters, H.J.; Bakker, K.J.; De Gijt, J.G.

    2013-01-01

    During the last two years, CUR committee 183 has worked on the upgrade of the Dutch Quay Walls handbook (CUR 211), which is to be published in 2013. Two of the main elements that are considered in this new edition are the addition of Finite Element analysis (FEM) as a method for design, comparable

  6. Regulation of the csgD promoter by global regulators HN-S, IHF, and RpoS in E. coli O157:H7 isolates

    Science.gov (United States)

    Introduction: Curli production is essential for the biofilm formation in E. coli and Salmonella. The control of expression of CsgD, the key regulator of the curli operon, is very complex, and has been shown to be regulated by several global regulators and is influenced by various growth and stress...

  7. Curcumin Attenuates Gentamicin-Induced Kidney Mitochondrial Alterations: Possible Role of a Mitochondrial Biogenesis Mechanism

    Directory of Open Access Journals (Sweden)

    Mario Negrette-Guzmán

    2015-01-01

    Full Text Available It has been shown that curcumin (CUR, a polyphenol derived from Curcuma longa, exerts a protective effect against gentamicin- (GM- induced nephrotoxicity in rats, associated with a preservation of the antioxidant status. Although mitochondrial dysfunction is a hallmark in the GM-induced renal injury, the role of CUR in mitochondrial protection has not been studied. In this work, LLC-PK1 cells were preincubated 24 h with CUR and then coincubated 48 h with CUR and 8 mM GM. Treatment with CUR attenuated GM-induced drop in cell viability and led to an increase in nuclear factor (erythroid-2-related factor 2 (Nrf2 nuclear accumulation and peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α cell expression attenuating GM-induced losses in these proteins. In vivo, Wistar rats were injected subcutaneously with GM (75 mg/Kg/12 h during 7 days to develop kidney mitochondrial alterations. CUR (400 mg/Kg/day was administered orally 5 days before and during the GM exposure. The GM-induced mitochondrial alterations in ultrastructure and bioenergetics as well as decrease in activities of respiratory complexes I and IV and induction of calcium-dependent permeability transition were mostly attenuated by CUR. Protection of CUR against GM-induced nephrotoxicity could be in part mediated by maintenance of mitochondrial functions and biogenesis with some participation of the nuclear factor Nrf2.

  8. Characteristics and cytotoxicity of folate-modified curcumin-loaded PLA-PEG micellar nano systems with various PLA:PEG ratios.

    Science.gov (United States)

    Phan, Quoc Thong; Le, Mai Huong; Le, Thi Thu Huong; Tran, Thi Hong Ha; Xuan, Phuc Nguyen; Ha, Phuong Thu

    2016-06-30

    Targeting delivery system use natural drugs for tumor cells is an appealing platform help to reduce the side effects and enhance the therapeutic effects of the drug. In this study, we synthesized curcumin (Cur) loaded (D, L Poly lactic - Poly ethylenglycol) micelle (Cur/PLA-PEG) with the ratio of PLA/PEG of 3:1 2:1 1:1 1:2 and 1:3 (w/w) and another micelle modified by folate (Cur/PLA-PEG-Fol) for targeting cancer therapy. The PLA-PEG copolymer was synthesized by ring opening polymerization method. After loading onto the micelle, solubility of Cur increased from 0.38 to 0.73mgml(-1). The average size of prepared Cur/PLA-PEG micelles was from 60 to 69nm (corresponding to the ratio difference of PLA/PEG) and the drug encapsulating efficiency was from 48.8 to 91.3%. Compared with the Cur/PLA-PEG micelles, the size of Cur/PLA-PEG-Fol micelles were from 80 to 86nm and showed better in vitro cellular uptake and cytotoxicity towards HepG2 cells. The cytotoxicity of the NPs however depends much on the PEG component. The results demonstrated that Folate-modified micelles could serve as a potential nano carrier to improve solubility, anti-cancer activity of Cur and targeting ability of the system. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. Curcumin Generates Oxidative Stress and Induces Apoptosis in Adult Schistosoma mansoni Worms

    Science.gov (United States)

    de Paula Aguiar, Daniela; Brunetto Moreira Moscardini, Mayara; Rezende Morais, Enyara; Graciano de Paula, Renato; Ferreira, Pedro Manuel; Afonso, Ana; Belo, Silvana; Tomie Ouchida, Amanda; Curti, Carlos; Cunha, Wilson Roberto; Rodrigues, Vanderlei

    2016-01-01

    Inducing apoptosis is an interesting therapeutic approach to develop drugs that act against helminthic parasites. Researchers have investigated how curcumin (CUR), a biologically active compound extracted from rhizomes of Curcuma longa, affects Schistosoma mansoni and several cancer cell lines. This study evaluates how CUR influences the induction of apoptosis and oxidative stress in couples of adult S. mansoni worms. CUR decreased the viability of adult worms and killed them. The tegument of the parasite suffered morphological changes, the mitochondria underwent alterations, and chromatin condensed. Different apoptotic parameters were determined in an attempt to understand how CUR affected adult S. mansoni worms. CUR induced DNA damage and fragmentation and increased the expression of SmCASP3/7 transcripts and the activity of Caspase 3 in female and male worms. However, CUR did not intensify the activity of Caspase 8 in female or male worms. Evaluation of the superoxide anion and different antioxidant enzymes helped to explore the mechanism of parasite death further. The level of superoxide anion and the activity of Superoxide Dismutase (SOD) increased, whereas the activity of Glutathione-S-Transferase (GST), Glutathione reductase (GR), and Glutathione peroxidase (GPX) decreased, which culminated in the oxidation of proteins in adult female and male worms incubated with CUR. In conclusion, CUR generated oxidative stress followed by apoptotic-like-events in both adult female and male S. mansoni worms, ultimately killing them. PMID:27875592

  10. Curcumin Generates Oxidative Stress and Induces Apoptosis in Adult Schistosoma mansoni Worms.

    Directory of Open Access Journals (Sweden)

    Daniela de Paula Aguiar

    Full Text Available Inducing apoptosis is an interesting therapeutic approach to develop drugs that act against helminthic parasites. Researchers have investigated how curcumin (CUR, a biologically active compound extracted from rhizomes of Curcuma longa, affects Schistosoma mansoni and several cancer cell lines. This study evaluates how CUR influences the induction of apoptosis and oxidative stress in couples of adult S. mansoni worms. CUR decreased the viability of adult worms and killed them. The tegument of the parasite suffered morphological changes, the mitochondria underwent alterations, and chromatin condensed. Different apoptotic parameters were determined in an attempt to understand how CUR affected adult S. mansoni worms. CUR induced DNA damage and fragmentation and increased the expression of SmCASP3/7 transcripts and the activity of Caspase 3 in female and male worms. However, CUR did not intensify the activity of Caspase 8 in female or male worms. Evaluation of the superoxide anion and different antioxidant enzymes helped to explore the mechanism of parasite death further. The level of superoxide anion and the activity of Superoxide Dismutase (SOD increased, whereas the activity of Glutathione-S-Transferase (GST, Glutathione reductase (GR, and Glutathione peroxidase (GPX decreased, which culminated in the oxidation of proteins in adult female and male worms incubated with CUR. In conclusion, CUR generated oxidative stress followed by apoptotic-like-events in both adult female and male S. mansoni worms, ultimately killing them.

  11. Final Environmental Assessment: For Construction at the Colorado Army National Guard Army Aviation Support Facility Complex, Buckley Air Force Base, Colorado

    Science.gov (United States)

    2005-01-01

    supports a small pocket of narrow-leafed cattail (Typha angustifolia) and scattered individuals of curly dock ( Rumex crispus ). Check holes dug in...Typha angustifolia) and scattered individuals of curly dock ( Rumex crispus ). Check holes dug in this area indicated reducing conditions and a hydric

  12. Progress in nanotechnology-based drug carrier in designing of curcumin nanomedicines for cancer therapy: current state-of-the-art.

    Science.gov (United States)

    Ahmad, Mohammad Zaki; Alkahtani, Saad Ahmed; Akhter, Sohail; Ahmad, Farhan Jalees; Ahmad, Javed; Akhtar, Mohammad Shabib; Mohsin, Nehal; Abdel-Wahab, Basel A

    2016-01-01

    Comprehensive pharmacological screening of curcumin (CUR) has given the evidence that it is an excellent naturally occurring therapeutic moiety for cancer. It is very well tolerated with insignificant toxicity even after high doses of oral administration. Irrespective of its better quality as an anticancer agent, therapeutic application of CUR is hampered by its extremely low-aqueous solubility and poor bioavailability, rapid clearance and low-cellular uptake. A simple means of breaking up the restrictive factor of CUR is to perk-up its aqueous solubility, improve its bioavailability, protect it from degradation, and metabolism and potentiate its targeting capacity towards the cancer cell. The development in the field of nanomedicine has made excellent progresses toward enhancing the bioavailability of lipophilic drugs like CUR. Nanoparticles (NPs) are capable to deliver the CUR at specific area and thereby prevent it from physiological degradation and systemic clearance. In recent year, an assortment of nanomedicine-based novel drug delivery system has been designed to potentiate the bioavailability of CUR towards anticancer therapy. In this review, we discuss the recent development in the field of nanoCUR (NanoCur), including polymeric micelles, liposome, polymeric NPs, nanoemulsion, nanosuspension, solid lipid NPs (SLNPs), polymer conjugates, nanogel, etc. in anticancer application.

  13. Telomerase: a target for therapeutic effects of curcumin and a curcumin derivative in Aβ1-42 insult in vitro.

    Directory of Open Access Journals (Sweden)

    Zijian Xiao

    Full Text Available This study was designed to investigate whether telomerase was involved in the neuroprotective effect of curcumin and Cur1. Alzheimer's disease is a consequence of an imbalance between the generation and clearance of amyloid-beta peptide in the brain. In this study, we used Aβ1-42 (10 µg/ml to establish a damaged cell model, and curcumin and Cur1 were used in treatment groups. We measured cell survival and cell growth, intracellular oxidative stress and hTERT expression. After RNA interference, the effects of curcumin and Cur1 on cells were verified. Exposure to Aβ1-42 resulted in significant oxidative stress and cell toxicity, and the expression of hTERT was significantly decreased. Curcumin and Cur1 both protected SK-N-SH cells from Aβ1-42 and up-regulated the expression of hTERT. Furthermore, Cur1 demonstrated stronger protective effects than curcumin. However, when telomerase was inhibited by TERT siRNA, the neuroprotection by curcumin and Cur1 were ceased. Our study indicated that the neuroprotective effects of curcumin and Cur1 depend on telomerase, and thus telomerase may be a target for therapeutic effects of curcumin and Cur1.

  14. Intraperitoneal curcumin decreased lung, renal and heart injury in abdominal aorta ischemia/reperfusion model in rat.

    Science.gov (United States)

    Aydin, Mehmet Salih; Caliskan, Ahmet; Kocarslan, Aydemir; Kocarslan, Sezen; Yildiz, Ali; Günay, Samil; Savik, Emin; Hazar, Abdussemet; Yalcin, Funda

    2014-01-01

    Previous studies have demonstrated that curcumin (CUR) has protective effects against ischemia reperfusion injury to various organs. We aimed to determine whether CUR has favorable effects on tissues and oxidative stress in abdominal aorta ischemia-reperfusion injury. Thirty rats were divided into three groups as sham, control and treatment (CUR) group. Control and CUR groups underwent abdominal aorta ischemia for 60 min followed by a 120 min period of reperfusion. In the CUR group, CUR was given 5 min before reperfusion at a dose of 200 mg/kg via an intraperitoneal route. Total antioxidant capacity (TAC), total oxidative status (TOS), and oxidative stress index (OSI) in blood serum were measured, and lung, renal and heart tissue histopathology were evaluated with light microscopy. TOS and OSI activity in blood samples were statistically decreased in sham and CUR groups compared to the control group (p OSI). Renal, lung, heart injury scores of sham and CUR groups were statistically decreased compared to control group (p model. Copyright © 2014 Surgical Associates Ltd. Published by Elsevier Ltd. All rights reserved.

  15. Epithelial cell adhesion molecule aptamer functionalized PLGA-lecithin-curcumin-PEG nanoparticles for targeted drug delivery to human colorectal adenocarcinoma cells

    Directory of Open Access Journals (Sweden)

    Li L

    2014-02-01

    Full Text Available Lei Li,1,* Dongxi Xiang,2,* Sarah Shigdar,2 Wenrong Yang,3 Qiong Li,2 Jia Lin,4 Kexin Liu,1 Wei Duan2 1College of Pharmacy, Dalian Medical University, Dalian, People's Republic of China; 2School of Medicine, Faculty of Health, Deakin University, Waurn Ponds, VIC, Australia; 3School of Life and Environmental Sciences, Faculty of Science, Engineering and Built Environment, Deakin University, Waurn Ponds, VIC, Australia; 4Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, People's Republic of China *These authors contributed equally to this work Abstract: To improve the efficacy of drug delivery, active targeted nanotechnology-based drug delivery systems are gaining considerable attention as they have the potential to reduce side effects, minimize toxicity, and improve efficacy of anticancer treatment. In this work CUR-NPs (curcumin-loaded lipid-polymer-lecithin hybrid nanoparticles were synthesized and functionalized with ribonucleic acid (RNA Aptamers (Apts against epithelial cell adhesion molecule (EpCAM for targeted delivery to colorectal adenocarcinoma cells. These CUR-encapsulated bioconjugates (Apt-CUR-NPs were characterized for particle size, zeta potential, drug encapsulation, stability, and release. The in vitro specific cell binding, cellular uptake, and cytotoxicity of Apt-CUR-NPs were also studied. The Apt-CUR-NP bioconjugates exhibited increased binding to HT29 colon cancer cells and enhancement in cellular uptake when compared to CUR-NPs functionalized with a control Apt (P<0.01. Furthermore, a substantial improvement in cytotoxicity was achieved toward HT29 cells with Apt-CUR-NP bioconjugates. The encapsulation of CUR in Apt-CUR-NPs resulted in the increased bioavailability of delivered CUR over a period of 24 hours compared to that of free CUR in vivo. These results show that the EpCAM Apt-functionalized CUR-NPs enhance the targeting and drug

  16. Preparation, characterization, and in vitro diffusion study of nonwoven electrospun nanofiber of curcumin-loaded cellulose acetate phthalate polymer

    Directory of Open Access Journals (Sweden)

    Rramaswamy Ravikumar

    2017-09-01

    Full Text Available Novel curcumin (CUR-loaded cellulose acetate phthalate (CAP nonwoven electrospun nanofiber (NF transdermal mat was developed and evaluated for its in vitro CUR diffusion properties. Various CAP solutions from 5 to 20 wt% were tested; 17.5 wt% was found to be a suitable concentration for NF fabrication without defects, such as bubble or ribbon structures. The selected wt% CAP solution was loaded with CUR and electrospun into NFs. The prepared CUR-loaded NFs were characterized using scanning electron microscopy, X-ray diffraction, ultraviolet–visible spectroscopy, thermogravimetric analysis (TGA, and in vitro diffusion studies. The as-prepared fibers demonstrated controlled in vitro transdermal delivery of CUR for up to 24 h.

  17. Preparation, characterization, and in vitro diffusion study of nonwoven electrospun nanofiber of curcumin-loaded cellulose acetate phthalate polymer.

    Science.gov (United States)

    Ravikumar, Rramaswamy; Ganesh, Mani; Ubaidulla, Udumansha; Young Choi, Eun; Tae Jang, Hyun

    2017-09-01

    Novel curcumin (CUR)-loaded cellulose acetate phthalate (CAP) nonwoven electrospun nanofiber (NF) transdermal mat was developed and evaluated for its in vitro CUR diffusion properties. Various CAP solutions from 5 to 20 wt% were tested; 17.5 wt% was found to be a suitable concentration for NF fabrication without defects, such as bubble or ribbon structures. The selected wt% CAP solution was loaded with CUR and electrospun into NFs. The prepared CUR-loaded NFs were characterized using scanning electron microscopy, X-ray diffraction, ultraviolet-visible spectroscopy, thermogravimetric analysis (TGA), and in vitro diffusion studies. The as-prepared fibers demonstrated controlled in vitro transdermal delivery of CUR for up to 24 h.

  18. Antifungal curcumin promotes chitin accumulation associated with decreased virulence of Sporothrix schenckii.

    Science.gov (United States)

    Huang, Lilin; Zhang, Jing; Song, Tianzhang; Yuan, Liyan; Zhou, Junjie; Yin, Hongling; He, Tailong; Gao, Wenchao; Sun, Yao; Hu, Xuchu; Huang, Huaiqiu

    2016-05-01

    Curcumin, a yellow polyphenol compound, is known to possess antifungal activity for a range of pathogenic fungi. However, the fungicidal mechanism of curcumin (CUR) has not been identified. We have occasionally found that chitin redistributes to the cell wall outer layer of Sporothrix schenckii (S. schenckii) upon sublethal CUR treatment. Whether CUR can affect chitin synthesis via the protein kinase C (PKC) signaling pathway has not been investigated. This study describes a direct fungicidal activity of CUR against S. schenckii demonstrated by the results of a checkerboard microdilution assay and, for the first time, a synergistic effect of CUR with terbinafine (TRB). Furthermore, the results of real-time PCR showed that sublethal CUR upregulated the transcription of PKC, chitin synthase1 (CHS1), and chitin synthase3 (CHS3) in S. schenckii. The fluorescence staining results using wheat germ agglutinin-fluorescein isothiocyanate (WGA-FITC) and calcofluor white (CFW) consistently showed that chitin exposure and total chitin content were increased on the conidial cell wall of S. schenckii by sublethal CUR treatment. A histopathological analysis of mice infected with CUR-treated conidia showed dampened inflammation in the local lesion and a reduced fungal burden. The ELISA results showed proinflammatory cytokine secretion at an early stage from macrophages stimulated by the CUR-treated conidia. The present data led to the conclusion that CUR is a potential antifungal agent and that its fungicidal mechanism may involve chitin accumulation on the cell wall of S. schenckii, which is associated with decreased virulence in infected mice. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Nanotechnology-based treatment for chemotherapy-resistant breast cancer

    Science.gov (United States)

    Abouzeid, Abraham H.; Patel, Niravkumar R.; Rachman, Ilya M.; Senn, Sean; Torchilin, Vladimir P.

    2014-08-01

    Background: Treatment of metastatic cancer remains a formidable clinical challenge. Better therapeutic options with improved tissue penetration and tumor cell uptake are urgently needed. Targeted nanotherapy, for improved delivery, and combinatory drug administration aimed at inhibiting chemo-resistance may be the solution. Purpose: The study was performed to evaluate the therapeutic efficacy of polymeric PEG-PE micelles, co-loaded with curcumin (CUR) and doxorubicin (DOX), and targeted with anti-GLUT1 antibody (GLUT1) against MDA-MB-231 human breast adenocarcinoma cells both in vitro and in vivo. Methods: MDA-MB-231 DOX-resistant cells were treated with non-targeted and GLUT1-targeted CUR and DOX micelles as a single agent or in combination. Tumor cells were also inoculated in female nude mice. Established tumors were treated with the micellar formulations at a dose of 6 mg/kg CUR and 1 mg/kg DOX every 2 d for a total of 7 injections. Results: CUR+DOX-loaded micelles decorated with GLUT1 had a robust killing effect even at low doses of DOX in vitro. At the doses chosen, non-targeted CUR and CUR+DOX micelles did not exhibit significant tumor inhibition versus control. However, GLUT1-CUR and GLUT1-CUR+DOX micelles showed a significant tumor inhibition effect with an improvement in survival. Conclusion: We showed a dramatic improvement in efficacy between the non-targeted and GLUT1-targeted formulations both in vitro and in vivo. Also, importantly, the addition of CUR to the micelle, has restored sensitivity to DOX, with resultant tumor growth inhibition. Hence, we confirmed that GLUT1-CUR+DOX micelles are effective in vitro and in vivo and deserve further investigation.

  20. Christ-Siemens-Touraine syndrome with cleft palate, absent nipples ...

    African Journals Online (AJOL)

    Rabah M. Shawky

    2015-06-27

    Jun 27, 2015 ... depressed nasal bridge, saddle nose, dry lips, angular stomati- tis, high arched palate ... normal. Figure 1. Sparse, thin dry hair over the scalp, scanty eyebrows and eyelashes, frontal bossing, prominent supraorbital ridges, depressed ..... [2] Weech AA. Hereditary ectodermal dysplasia (congenital ectoder-.

  1. Recovery of orbital fat pad prolapsus and deepening of the lid sulcus from topical bimatoprost therapy: 2 case reports and review of the literature.

    Science.gov (United States)

    Aydin, Sayime; Işikligil, Işil; Tekşen, Yasemin Aktan; Kir, Erkin

    2010-09-01

    To report 2 cases of resolution of orbital fat pad prolapsus and deepening of the lid sulcus caused by topical bimatoprost therapy, and to review the literature. This was a retrospective, observational chart review. A 54-year-old man with normotensive glaucoma presented with recovery of left lower lid orbital fat pad prolapsus, deepening of the lid sulcus, and iris color and eyelash changes 8 months after initiation of bimatoprost therapy in his left eye (OS). After stopping bimatoprost, his eyelashes and the lower lid orbital fat pad partially regained their natural appearance at 2 months and 4 months, respectively. However, there was no improvement of the left upper eyelid deepening and the iris hyperpigmentation, even after 30 months. A 75-year-old man with unilateral pseudoexfoliative glaucoma had been treated with bimatoprost OS. Three months after initiation of the therapy, his examination showed recovery of the inferior and superomedial orbital fat pad prolapsus, a deep and prominent upper lid sulcus with eyelash changes, and iris hyperpigmentation OS. Five months after discontinuation of bimatoprost, the eyelashes and superomedial orbital fat pad improved. Twenty-four months later, the inferior orbital fat pad partially got its natural appearance back, but the upper lid sulcus was slightly more deep and prominent than the fellow right eye. There was no alteration in iris hyperpigmentation. Clinicians and patients should be aware of these possible effects of topical bimatoprost therapy. These adverse effects may not be completely reversible after discontinuation of the medication.

  2. Sammenlimning af begge øjne skal ikke behandles konservativt

    DEFF Research Database (Denmark)

    Øgard, Carsten; Kjaerbo, Hadi; Sørensen, Torben Lykke

    2010-01-01

    We describe a case of a 4-year-old boy who accidentally had both eyes glued together. The eyelashes were cut and the boy's eyelids were separated in general anaesthesia. When both eyes are glued together, we do not recommend expectancy, especially not in paediatric cases. For psychological reason...

  3. KERATOSIS FOLLICULARIS SPINULOSA DECALVANS: REPORT OF A CASE AND LITERATURE REVIEW

    Directory of Open Access Journals (Sweden)

    P. Mansouri.

    1999-07-01

    Full Text Available Keratosis follicularis spinulosa decalvans (KFSD represents a rare, probably X-linked recessive genodermatosis, characterized by keratosis pilaris of face, trunk and extremities, followed by atrophy, cicatricial alopecia of the scalp, eyebrows and eyelashes, photophobia and corneal abnormalities. We report a rare case of KFSD and review the literature.

  4. 21 CFR 73.2190 - Henna.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Henna. 73.2190 Section 73.2190 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR ADDITIVES... safely used for coloring hair only. It may not be used for coloring the eyelashes or eyebrows, or...

  5. Microcephalic osteodysplastic primordial dwarfism (MOPD) type I ...

    African Journals Online (AJOL)

    We report a 4 month old male, 4th in order of birth of healthy consanguineous Egyptian parents with typical characteristics of microcephalic osteodysplastic primordial dwarfism most probably belongs to type I (MOPD I). The patient had intrauterine growth retardation, sparse scalp hair, sparse eyebrows and eyelashes, high ...

  6. In silico modeling of lipase H | Jabeen | African Journal of ...

    African Journals Online (AJOL)

    LAH 2 is a type of autosomal recessive hypotrichosis that affect hairs, eyebrows, scalp and eyelashes. Mutations in Lipase H gene result in LAH 2. Changes that result from mutation on physiochemical properties, post-translational modifications, functional sites, secondary structure and tertiary structure lipase H gene (LIPH) ...

  7. Chemotherapy and Hair Loss: What to Expect during Treatment

    Science.gov (United States)

    ... Sometimes your eyelash, eyebrow, armpit, pubic and other body hair also falls out. Some chemotherapy drugs are more ... dose of chemotherapy as the rest of your body. People undergoing scalp hypothermia ... a drug approved for hair loss — to your scalp before and during chemotherapy ...

  8. Comparison of two surgical treatments for pediatric lower eyelid trichiasis

    Directory of Open Access Journals (Sweden)

    Yan-Xia Xiao

    2014-06-01

    Full Text Available AIM: To compare the clinical results of suture method and partial eyelash resection treating for pediatric eyelid trichiasis, and screen an effective method for the treatment of pediatric lower eyelid trichiasis. METHODS: Fifty-six cases of pediatric patients with lower eyelid trichiasis were randomly divided into a control group and an observation group in accordance with the method of drawing lots, and each group was 28 cases. The control group was treated with suture method, and the observation group was treated with partial eyelash resection. The clinical efficacy, patient satisfaction before and after treatment, and the incidence of complications were compared. RESULTS:(1The clinically total effective rate was 74% of the control group, which was 89% of the observation group, and there were statistical differences of the clinical efficacy between the two groups(PPPPCONCLUSION: The treatment of children with lower eyelid trichiasis, suture method is simple and can be performed under local anesthesia in collaboration with children, but with a higher relapse rate, some patients required reoperation; partial resection of eyelashes can be more thoroughly solve the problem of pediatric eyelid trichiasis with low recurrence rate, but children need to be under general anesthesia with some of big risk. So partial resection of eyelashes is unsuitable for using in clinical practice widely and can be used in special cases.

  9. 21 CFR 73.2396 - Lead acetate.

    Science.gov (United States)

    2010-04-01

    ... Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR... mustaches, eyelashes, eyebrows, or hair on parts of the body other than the scalp. (d) Labeling requirements... displayed thereon: CAUTION: Contains lead acetate. For external use only. Keep this product out of children...

  10. Body image and psychological distress in women with breast cancer: a French online survey on patients' perceptions and expectations.

    Science.gov (United States)

    Pierrisnard, Camille; Baciuchka, Marjorie; Mancini, Julien; Rathelot, Pascal; Vanelle, Patrice; Montana, Marc

    2017-12-29

    Altered body image caused by alopecia, loss of eyebrows or eyelashes, or mastectomy is a major source of psychological distress in women with breast cancer. To identify and to assess patients' perceptions and expectations regarding altered body image. Opinion survey conducted among patients treated for breast cancer and member of French online support groups. Anonymous online self-administered survey sent to women with breast cancer. 85% of the women interviewed experienced alopecia during treatment and 67% of them loss of eyebrows or eyelashes. About half of patients suffering alopecia and loss of eyebrows or eyelashes reported fearing what others think. Mastectomy was experienced by 84% of the women in our study, but only 32% of them reported fearing what others think. 87% of our study cohort received information about the possibility of adverse events. 70, 56, and 60% of women felt helped by information they received for the management of alopecia, loss of eyebrows or eyelashes, or mastectomy, respectively. This study confirms that altered body image is a critical psychosocial issue for women with breast cancer. Effective information can be a source of reassurance and may constitute one of the most important sources of emotional support.

  11. Disease: H01906 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H01906 Poikiloderma, hereditary fibrosing, with tendon contractures, myopathy, and... pulmonary fibrosis Poikiloderma, hereditary fibrosing, with tendon contractures, myopathy, and pulmonary fi...ies especially on the face and sun-exposed areas. Scalp hair, eyelashes, and eyebrows are typically sparse. Tendon contract

  12. A Study of Kaduna Polytechnic, Kaduna

    African Journals Online (AJOL)

    2018-01-30

    Jan 30, 2018 ... the age range was 16–52 with a mean of 23.0 years ± 4.8. The prevalence of eyelash use was 38.7%. Beauty was the most common reason for lash extension. 56.1% (n = 174). Others were curiosity, peer pressure, and replacement therapy. The most common complication was itching 45.8% (n = 142).

  13. Christ-Siemens-Touraine syndrome with cleft palate, absent nipples ...

    African Journals Online (AJOL)

    We report a 6 year old child, second in order of birth of non consanguineous Egyptian parents with typical characteristics of Christ-Siemens-Touraine syndrome. The patient had sparse light hair over the scalp, scanty eyebrows and eyelashes, a high arched cleft palate, decayed oligodontic teeth, hyperpigmentation all over ...

  14. Comparison of features response in texture-based iris segmentation

    CSIR Research Space (South Africa)

    Bachoo, A

    2009-03-01

    Full Text Available and eyelashes that corrupt the iris region of interest. An accurate segmentation algorithm must localize and remove these noise components. Texture features are considered in this paper for describing iris and non-iris regions. These regions are classified using...

  15. Tactics Young Women Use to Resist Condom Use When a Partner Wants to Use a Condom.

    Science.gov (United States)

    Wegner, Rhiana; Lewis, Melissa A; Davis, Kelly Cue; Neilson, Elizabeth C; Norris, Jeanette

    2017-10-18

    Although there is a growing literature on men's condom use resistance (CUR) tactics (e.g., direct requests, deception), little research exists on women's CUR tactics. This study investigated young women's (ages 18 to 21) self-reported use of CUR tactics since age 14 and related individual difference factors. Participants included 235 sexually active heterosexual women from a nationwide convenience survey sample who completed a newly adapted women's version of the Condom Use Resistance Survey. Consistent with the limited previous research, women were most likely to use risk-level reassurance (37.9%) and seduction (33.2%) tactics. A higher frequency and quantity of alcohol consumption, particularly prior to sex, lower perceived risk of sexually transmitted infections (STIs), and a history of STI diagnosis were associated with having previously used a greater number and variety of CUR tactics. This highlights the need for CUR prevention and intervention programming for women. Future research should specifically examine women's rationale for using CUR tactics and utilize longitudinal and experimental methods to further elucidate directional and causal relationships among individual-level risk factors, CUR, and negative sexual health outcomes.

  16. Solid Lipid Curcumin Particles Induce More DNA Fragmentation and Cell Death in Cultured Human Glioblastoma Cells than Does Natural Curcumin

    Directory of Open Access Journals (Sweden)

    Panchanan Maiti

    2017-01-01

    Full Text Available Despite recent advancements in cancer therapies, glioblastoma multiforme (GBM remains largely incurable. Curcumin (Cur, a natural polyphenol, has potent anticancer effects against several malignancies, including metastatic brain tumors. However, its limited bioavailability reduces its efficiency for treating GBM. Recently, we have shown that solid lipid Cur particles (SLCPs have greater bioavailability and brain tissue penetration. The present study compares the efficiency of cell death by Cur and/or SLCPs in cultured GBM cells derived from human (U-87MG and mouse (GL261 tissues. Several cell viability and cell death assays and marker proteins (MTT assay, annexin-V staining, TUNEL staining, comet assay, DNA gel electrophoresis, and Western blot were investigated following the treatment of Cur and/or SLCP (25 μM for 24–72 h. Relative to Cur, the use of SLCP increased cell death and DNA fragmentation, produced longer DNA tails, and induced more fragmented nuclear lobes. In addition, cultured GBM cells had increased levels of caspase-3, Bax, and p53, with decreases in Bcl2, c-Myc, and both total Akt, as well as phosphorylated Akt, when SLCP, rather Cur, was used. Our in vitro work suggests that the use of SLCP may be a promising strategy for reversing or preventing GBM growth, as compared to using Cur.

  17. Pre-treatment with curcumin modulates acetylcholinesterase activity and proinflammatory cytokines in rats infected with Trypanosoma evansi.

    Science.gov (United States)

    Wolkmer, Patrícia; Silva, Cássia B da; Paim, Francine C; Duarte, Marta M M F; Castro, Verônica; Palma, Heloisa E; França, Raqueli T; Felin, Diandra V; Siqueira, Lucas C; Lopes, Sonia T A; Schetinger, Maria Rosa C; Monteiro, Silvia G; Mazzanti, Cinthia M

    2013-04-01

    The potent activity against Trypanosomes and health beneficial effects of curcumin (Cur) has been demonstrated in various experimental models. In this study, we evaluated the in vivo effect of Cur as trypanocide and as potential anti-inflammatory agent, through the evaluation of immunomodulatory mechanisms in rats infected with Trypanosoma evansi. Daily oral Cur was administered at doses of 0, 20 or 60mg/kg as preventive treatment (30 and 15days pre infection) and as treatment (post infection). The treatment of the groups continued until the day of euthanasia. Fifteen days after inoculation, parasitemia, plasma proinflammatory cytokines (IFN-γ, TNF-α, IL-1, IL-6), anti-inflammatory cytokines (IL-10) and blood acetylcholinesterase activity (AChE) were analyzed. Pretreatment with Cur reduced parasitemia and lethality. Cur inhibited AChE activity and improved immunological response by cytokines proinflammatory, fundamental during T. evansi infection. We found that Cur is not so important as an antitrypanosomal activity but as immunomodulator agent. These findings reveal that the preventive use of Cur stimulates anti-inflammatory mechanisms, reducing an excessive inflammatory response. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  18. Development of Food-Grade Curcumin Nanoemulsion and its Potential Application to Food Beverage System: Antioxidant Property and In Vitro Digestion.

    Science.gov (United States)

    Joung, Hee Joung; Choi, Mi-Jung; Kim, Jun Tae; Park, Seok Hoon; Park, Hyun Jin; Shin, Gye Hwa

    2016-03-01

    Curcumin nanoemulsions (Cur-NEs) were developed with various surfactant concentrations by using high pressure homogenization and finally applied to the commercial milk system. Characterization of Cur-NEs was performed by measuring the droplet size and polydispersity index value at different Tween 20 concentrations. The morphology of the Cur-NEs was observed by confocal laser scanning microscopy and transmission electron microscopy. Antioxidant activity and in vitro digestion ability were tested using 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, pH-stat method, and thiobarbituric acid reactive substances assays. Cur-NEs were found to be physically stable for 1 mo at room temperature. The surfactant concentration affects particle formation and droplet size. The mean droplet size decreased from 122 to 90 nm when surfactant concentration increased 3 times. Cur-NEs had shown an effective oxygen scavenging activity. Cur-NEs-fortified milk showed significantly lower lipid oxidation than control (unfortified) milk and milk containing curcumin-free nanoemulsions. These properties make Cur-NEs suitable systems for the beverage industry. © 2016 Institute of Food Technologists®

  19. Preparation and anti-cancer activity of polymer-encapsulated curcumin nanoparticles

    International Nuclear Information System (INIS)

    Ha, Phuong Thu; Tran, Dai Lam; Nguyen, Xuan Phuc; Le, Mai Huong; Ha Tran, Thi Hong; Hoang, Thi My Nhung; Huong Le, Thi Thu; Duong, Tuan Quang

    2012-01-01

    Curcumin (Cur) is a yellow compound isolated from rhizome of the herb curcuma longa. Curcumin possesses antioxidant, anti-inflammatory, anti-carcinogenic and antimicrobial properties, and suppresses proliferation of many tumor cells. However, the clinical application of curcumin in cancer treatment is considerably limited due to its serious poor delivery characteristics. In order to increase the hydrophilicity and drug delivery capability, we encapsulated curcumin into copolymer PLA-TPGS, 1,3-beta-glucan (Glu), O-carboxymethyl chitosan (OCMCs) and folate-conjugated OCMCs (OCMCs-Fol). These polymer-encapsulated curcumin nanoparticles (Cur-PLA-TPGS, Cur-Glu, Cur-OCMCs and Cur-OCMCs-Fol) were characterized by infrared (IR), fluorescence (FL), photoluminescence (PL) spectra, field emission scanning electron microscopy (FE-SEM), and found to be spherical particles with an average size of 50–100 nm, being suitable for drug delivery applications. They were much more soluble in water than not only free curcumin but also other biodegradable polymer-encapsulated curcumin nanoparticles. The anti-tumor promoting assay was carried out, showing the positive effects of Cur-Glu and Cur-PLA-TPGS on tumor promotion of Hep-G2 cell line in vitro. Confocal microscopy revealed that the nano-sized curcumin encapsulated by polymers OCMCs and OCMCs-Fol significantly enhanced the cellular uptake (cancer cell HT29 and HeLa). (paper)

  20. Preparation and anti-cancer activity of polymer-encapsulated curcumin nanoparticles

    Science.gov (United States)

    Thu Ha, Phuong; Huong Le, Mai; Nhung Hoang, Thi My; Thu Huong Le, Thi; Quang Duong, Tuan; Tran, Thi Hong Ha; Tran, Dai Lam; Phuc Nguyen, Xuan

    2012-09-01

    Curcumin (Cur) is a yellow compound isolated from rhizome of the herb curcuma longa. Curcumin possesses antioxidant, anti-inflammatory, anti-carcinogenic and antimicrobial properties, and suppresses proliferation of many tumor cells. However, the clinical application of curcumin in cancer treatment is considerably limited due to its serious poor delivery characteristics. In order to increase the hydrophilicity and drug delivery capability, we encapsulated curcumin into copolymer PLA-TPGS, 1,3-beta-glucan (Glu), O-carboxymethyl chitosan (OCMCs) and folate-conjugated OCMCs (OCMCs-Fol). These polymer-encapsulated curcumin nanoparticles (Cur-PLA-TPGS, Cur-Glu, Cur-OCMCs and Cur-OCMCs-Fol) were characterized by infrared (IR), fluorescence (FL), photoluminescence (PL) spectra, field emission scanning electron microscopy (FE-SEM), and found to be spherical particles with an average size of 50-100 nm, being suitable for drug delivery applications. They were much more soluble in water than not only free curcumin but also other biodegradable polymer-encapsulated curcumin nanoparticles. The anti-tumor promoting assay was carried out, showing the positive effects of Cur-Glu and Cur-PLA-TPGS on tumor promotion of Hep-G2 cell line in vitro. Confocal microscopy revealed that the nano-sized curcumin encapsulated by polymers OCMCs and OCMCs-Fol significantly enhanced the cellular uptake (cancer cell HT29 and HeLa).

  1. A Glycyrrhetinic Acid-Modified Curcumin Supramolecular Hydrogel for liver tumor targeting therapy

    Science.gov (United States)

    Chen, Guoqin; Li, Jinliang; Cai, Yanbin; Zhan, Jie; Gao, Jie; Song, Mingcai; Shi, Yang; Yang, Zhimou

    2017-03-01

    Curcumin (Cur), a phenolic anti-oxidant compound obtained from Curcuma longa plant, possesses a variety of therapeutic properties. However, it is suffered from its low water solubility and low bioavailability property, which seriously restricts its clinical application. In this study, we developed a glycyrrhetinic acid (GA) modified curcumin supramolecular pro-gelator (GA-Cur) and a control compound Nap-Cur by replacing GA with the naphthylacetic acid (Nap). Both compounds showed good water solubility and could form supramolecular gels by disulfide bond reduction triggered by glutathione (GSH) in vitro. Both formed gels could sustainedly release Cur in buffer solutions. We also investigated the cytotoxicity of pro-gelators to HepG2 cells by a MTT assay and determined the cellular uptake behaviours of them by fluorescence microscopy and LC-MS. Due to the over expression of GA receptor in liver cancer cells, our pro-gelator of GA-Cur showed an enhanced cellular uptake and better inhibition capacity to liver tumor cells than Nap-Cur. Therefore, the GA-Cur could significantly inhibit HepG2 cell growth. Our study provides a novel nanomaterial for liver tumor chemotherapy.

  2. One-step formation of lipid-polyacrylic acid-calcium carbonate nanoparticles for co-delivery of doxorubicin and curcumin.

    Science.gov (United States)

    Peng, Jianqing; Fumoto, Shintaro; Miyamoto, Hirotaka; Chen, Yi; Kuroda, Naotaka; Nishida, Koyo

    2017-09-01

    A doxorubicin (Dox) and curcumin (Cur) combination treatment regimen has been widely studied in pre-clinical research. However, the nanoparticles developed for this combination therapy require a consecutive drug loading process because of the different water-solubility of these drugs. This study provides a strategy for the "one-step" formation of nanoparticles encapsulating both Dox and Cur. We took advantage of polyacrylic acid (PAA) and calcium carbonate (CaCO 3 ) to realise a high drug entrapment efficiency (EE) and pH-sensitive drug release using a simplified preparation method. Optimisation of lipid ratios and concentrations of CaCO 3 was conducted. Under optimal conditions, the mean diameter of PEGylated lipid/PAA/CaCO 3 nanoparticles with encapsulated Cur and Dox (LPCCD) was less than 100 nm. An obvious pH-sensitive release of both drugs was observed, with different Dox and Cur release rates. Successful co-delivery of Cur and Dox was achieved via LPCCD on HepG2 cells. LPCCD altered the bio-distribution of Dox and Cur in vivo and decreased Dox-induced cardiotoxicity. The current investigation has developed an efficient ternary system for co-delivery of Dox and Cur to tumours, using a "one-step" formation resulting in nanoparticles possessing remarkable pH-sensitive drug release behaviour, which may be valuable for further clinical studies and eventual clinical application.

  3. Metabolomics Reveals Metabolic Targets and Biphasic Responses in Breast Cancer Cells Treated by Curcumin Alone and in Association with Docetaxel

    Science.gov (United States)

    2013-01-01

    Background Curcumin (CUR) has deserved extensive research due to its anti-inflammatory properties, of interest in human diseases including cancer. However, pleiotropic even paradoxical responses of tumor cells have been reported, and the mechanisms of action of CUR remain uncompletely elucidated. Methodology/Principal Findings 1H-NMR spectroscopy-based metabolomics was applied to get novel insight into responses of MCF7 and MDA-MB-231 breast cancer cells to CUR alone, and MCF7 cells to CUR in cotreatment with docetaxel (DTX). In both cell types, a major target of CUR was glutathione metabolism. Total glutathione (GSx) increased at low dose CUR (≤ 10 mg.l−1–28 µM-) (up to +121% in MCF7 cells, Phomocystein, creatine and taurine (−60 to −80%, all, P<0.05). Together with glutathione-S-transferase actvity, data established that GSx biosynthesis was upregulated at low dose, and GSx consumption activated at high dose. Another major target, in both cell types, was lipid metabolism involving, at high doses, accumulation of polyunsaturated and total free fatty acids (between ×4.5 and ×11, P<0.025), and decrease of glycerophospho-ethanolamine and -choline (about −60%, P<0.025). Multivariate statistical analyses showed a metabolic transition, even a biphasic behavior of some metabolites including GSx, between low and high doses. In addition, CUR at 10 mg.l−1 in cotreatment with DTX induced modifications in glutathione metabolism, lipid metabolism, and glucose utilization. Some of these changes were biphasic depending on the duration of exposure to CUR. Conclusions/Significance Metabolomics reveals major metabolic targets of CUR in breast cancer cells, and biphasic responses that challenge the widely accepted beneficial effects of the phytochemical. PMID:23472124

  4. Discovery and in Vivo Evaluation of Novel RGD-Modified Lipid-Polymer Hybrid Nanoparticles for Targeted Drug Delivery

    Directory of Open Access Journals (Sweden)

    Yinbo Zhao

    2014-09-01

    Full Text Available In the current study, the lipid-shell and polymer-core hybrid nanoparticles (lpNPs modified by Arg–Gly–Asp(RGD peptide, loaded with curcumin (Cur, were developed by emulsification-solvent volatilization method. The RGD-modified hybrid nanoparticles (RGD–lpNPs could overcome the poor water solubility of Cur to meet the requirement of intravenous administration and tumor active targeting. The obtained optimal RGD-lpNPs, composed of PLGA (poly(lactic-co-glycolic acid–mPEG (methoxyl poly(ethylene- glycol, RGD–polyethylene glycol (PEG–cholesterol (Chol copolymers and lipids, had good entrapment efficiency, submicron size and negatively neutral surface charge. The core-shell structure of RGD–lpNPs was verified by TEM. Cytotoxicity analysis demonstrated that the RGD–lpNPs encapsulated Cur retained potent anti-tumor effects. Flow cytometry analysis revealed the cellular uptake of Cur encapsulated in the RGD–lpNPs was increased for human umbilical vein endothelial cells (HUVEC. Furthermore, Cur loaded RGD–lpNPs were more effective in inhibiting tumor growth in a subcutaneous B16 melanoma tumor model. The results of immunofluorescent and immunohistochemical studies by Cur loaded RGD–lpNPs therapies indicated that more apoptotic cells, fewer microvessels, and fewer proliferation-positive cells were observed. In conclusion, RGD–lpNPs encapsulating Cur were developed with enhanced anti-tumor activity in melanoma, and Cur loaded RGD–lpNPs represent an excellent tumor targeted formulation of Cur which might be an attractive candidate for cancer therapy.

  5. Neuromuscular blockade of slow twitch muscle fibres elevates muscle oxygen uptake and energy turnover during submaximal exercise in humans

    DEFF Research Database (Denmark)

    Krustrup, Peter; Secher, Niels; Relu, Mihai U.

    2008-01-01

    without (CON) and with (CUR) arterial injections of the non-depolarizing neuromuscular blocking agent cisatracurium. In CUR, creatine phosphate (CP) was unaltered in slow twitch (ST) fibres and decreased (P CON, CP decreased (P ... fibres, respectively. From 127 s of exercise, muscle VO2 was higher (P CON (425 +/- 25 (+/- S.E.M.) versus 332 +/- 30 ml min(-1)) and remained higher (P ... was slower (P CON (55 +/- 6 versus 33 +/- 5 s). During CUR and CON, muscle homogenate CP was lowered (P CON (37.8 +/- 4.1 versus 35.2 +/- 6.2 mmol). Estimated total muscle ATP turnover was 19...

  6. Curcumin attenuated paracetamol overdose induced hepatitis

    Science.gov (United States)

    Somanawat, Kanjana; Thong-Ngam, Duangporn; Klaikeaw, Naruemon

    2013-01-01

    AIM: To investigate whether curcumin could attenuate hepatitis in mice with paracetamol overdose. METHODS: Male mice were divided into four groups. Group 1 (control, n = 8); was fed with distilled water; Group 2 [N-acetyl-P-aminophenol (APAP), n = 8]; was fed with a single dose of 400 mg/kg APAP dissolved in distilled water; Group 3 [APAP + curcumin (CUR) 200, n = 8], was fed with a single dose of 400 mg/kg APAP and 200 mg/kg CUR; Group 4 (APAP + CUR 600, n = 8), was fed with a single dose of 400 mg/kg APAP and 600 mg/kg CUR. Twenty-four hours later, the liver was removed to examine hepatic glutathione (GSH), hepatic malondialdehyde (MDA), and histopathologically. Then whole blood was withdrawn from heart to determine transaminase (serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase) and inflammatory cytokines [interleukin (IL)-12 and IL-18] levels by enzyme linked immunosorbent assay. RESULTS: Serum transaminase, hepatic MDA, and inflammatory cytokines increased significantly in the APAP compared with the control group. Curcumin supplementation in APAP + CUR 200 and APAP + CUR 600 groups significantly decreased these parameters compared with the APAP group. The level of GSH decreased significantly in the APAP compared with the control group. Curcumin supplementation in APAP + CUR 200 and APAP + CUR 600 groups significantly increased these parameters compared with the APAP group. The histological appearance of the liver in the control group showed normal. In the APAP-treated group, the liver showed extensive hemorrhagic hepatic necrosis at all zones. Curcumin supplementation in APAP + CUR 200 and APAP + CUR 600 groups, caused the liver histopathology to improve. In the APAP + CUR 200 group, the liver showed focal necrosis and but the normal architecture was well preserved in APAP + CUR 600 group. CONCLUSION: APAP overdose can cause liver injury. Results indicate that curcumin prevents APAP-induced hepatitis through the improvement of

  7. Hyaluronic acid-functionalized polymeric nanoparticles for colon cancer-targeted combination chemotherapy

    Science.gov (United States)

    Xiao, Bo; Han, Moon Kwon; Viennois, Emilie; Wang, Lixin; Zhang, Mingzhen; Si, Xiaoying; Merlin, Didier

    2015-10-01

    Nanoparticle (NP)-based combination chemotherapy has been proposed as an effective strategy for achieving synergistic effects and targeted drug delivery for colon cancer therapy. Here, we fabricated a series of hyaluronic acid (HA)-functionalized camptothecin (CPT)/curcumin (CUR)-loaded polymeric NPs (HA-CPT/CUR-NPs) with various weight ratios of CPT to CUR (1 : 1, 2 : 1 and 4 : 1). The resultant spherical HA-CPT/CUR-NPs had a desirable particle size (around 289 nm), relative narrow size distribution, and slightly negative zeta potential. These NPs exhibited a simultaneous sustained release profile for both drugs throughout the time frame examined. Subsequent cellular uptake experiments demonstrated that the introduction of HA to the NP surface endowed NPs with colon cancer-targeting capability and markedly increased cellular uptake efficiency compared with chitosan-coated NPs. Importantly, the combined delivery of CPT and CUR in one HA-functionalized NP exerted strong synergistic effects. HA-CPT/CUR-NP (1 : 1) showed the highest antitumor activity among the three HA-CPT/CUR-NPs, resulting in an extremely low combination index. Collectively, our findings indicate that this HA-CPT/CUR-NP can be exploited as an efficient formulation for colon cancer-targeted combination chemotherapy.Nanoparticle (NP)-based combination chemotherapy has been proposed as an effective strategy for achieving synergistic effects and targeted drug delivery for colon cancer therapy. Here, we fabricated a series of hyaluronic acid (HA)-functionalized camptothecin (CPT)/curcumin (CUR)-loaded polymeric NPs (HA-CPT/CUR-NPs) with various weight ratios of CPT to CUR (1 : 1, 2 : 1 and 4 : 1). The resultant spherical HA-CPT/CUR-NPs had a desirable particle size (around 289 nm), relative narrow size distribution, and slightly negative zeta potential. These NPs exhibited a simultaneous sustained release profile for both drugs throughout the time frame examined. Subsequent cellular uptake experiments

  8. El Derecho a la Salud en Latinoamérica y la Unión Europea. Accesibilidad en contextos de crisis

    OpenAIRE

    Quercetti, Florencia

    2014-01-01

    Treballs finals del Màster "Estudios Internacionales: organizaciones internacionales y cooperación – Colección Memorias MEI", Facultat de Dret, Universitat de Barcelona, Curs:2013-2014 , Directora: Marta Ortega Gomez

  9. Modified gum Arabic hydrogels as matrices for controlled release of curcumin supramolecular complexes

    International Nuclear Information System (INIS)

    Gerola, Adriana P.; Silva, Danielle C.; Rubira, Adley F.; Muniz, Edvani C.

    2015-01-01

    Modified gum Arabic (GA) hydrogels show a pH-responsive behavior making them excellent matrices to be used for oral administration of drugs. Our goal is to study the behavior of those matrices in simulated gastric and intestinal fluids. In this work we will present how the methacrylation degree of GA, by using glycidyl methacrylate, can affect the properties of these hydrogels for controlled release. The drug used in this work is the curcumin (Cur). Cur is associated with numerous pharmacological activities, but their application is limited by the low water solubility. We will present some studies involving the formation of host-guest complexes between Cur and natural cyclodextrins. Both modified GA and hydrogels were characterized by different techniques. The kinetics release of Cur complex-containing modified GA hydrogels was studied to have an insight on the release mechanism and rate constants. Toxicity studies on undifferentiated and differentiated Caco-2 were also carried out. (author)

  10. Las sombras de la Sagrada Familia

    OpenAIRE

    Castaño Ramos, Anaïs Tania; Coca Lozano, Marta; Ensenyat Rubí, Àlex; Sabariego Aguilar, Adriana; Villaverde Ruiz, Víctor

    2016-01-01

    Treballs de l'alumnat del Grau de Comunicació Audiovisual, Facultat de Biblioteconomia i Documentació, Universitat de Barcelona, [Projectes I - Grup II]. Curs: 2015-2016, Tutor: Jaume Vilaseca Corderroure.

  11. Estándares internacionales del trabajo infantil: el caso de Costa Rica

    OpenAIRE

    Salvador Ferrer, Ángel

    2015-01-01

    Treballs finals del Màster "Estudios Internacionales: organizaciones internacionales y cooperación – Colección Memorias MEI", Facultat de Dret, Universitat de Barcelona, Curs:2013-2014 , Tutor: Jordi Bonet Pérez

  12. Acute and chronic urine retention among adults at the urology ...

    African Journals Online (AJOL)

    E.M.T. Yenli

    AUR) and chronic urine retention. (CUR) are different and varied in both gender and age. Urine retention has been well studied among males worldwide, while data on urine retention among women are sparse. This study aimed at determining the.

  13. La seguridad alimentaria en el África Subsahariana y la cooperación Sur-Sur brasileña en materia agrícola: potencialidades y límites

    OpenAIRE

    De Andrade e Sousa, Maria Eduarda

    2014-01-01

    Treballs finals del Màster "Estudios Internacionales: organizaciones internacionales y cooperación – Colección Memorias MEI", Facultat de Dret, Universitat de Barcelona, Curs:2013-2014 , Director: Xavier Fernández Pons

  14. Folliculitis

    Science.gov (United States)

    ... sometimes develops if you're receiving long-term antibiotic therapy for acne. Boils (furuncles) and carbuncles. These occur ... medications, such as steroid creams or long-term antibiotic therapy for acne Being a male with curly hair ...

  15. Tribal Decisions-Makers Guide to Solid Waste Management: Chapter 2 - Developing Solid Waste Management Plans

    Science.gov (United States)

    Solid waste management plans offer a host of benefits for tribes and Alaskan Native villages. Through the preparation of these plans, you can assess your cur-rent and future waste management needs, set priorities, and allocate resources accordingly.

  16. Children with bronchial asthma assessed for psychosocial problems ...

    African Journals Online (AJOL)

    .5,8 Social problems that may oc- cur in asthma are also many, these include: educational problems, family dysfunction, stigma, parental distress, sibling resentment and jealousy, maternal over protec- tiveness and altered relationships with ...

  17. Descobrim les emocions amb l’art. Proposta d’activitats educatives per alumnat d’Educació Primària

    OpenAIRE

    Roger Lorenzo, Inmaculada

    2007-01-01

    Postgrau en Educació Emocional i Benestar, Facultat de Pedagogia, Departament de Mètodes d’Investigació i Diagnòstic en Educació, Universitat de Barcelona, curs: 2006-2007, Tutor: Rafel Bisquerra Alzina

  18. L’educació emocional en el context educatiu (Educació en valors i convivència en els centres)

    OpenAIRE

    Gaya-Catasus, Jaume

    2004-01-01

    Postgrau en Educació Emocional i Benestar, Facultat de Pedagogia, Departament de Mètodes d’Investigació i Diagnòstic en Educació, Universitat de Barcelona, curs: 2003-2004, Tutor: Rafel Bisquerra Alzina

  19. Successful treatment of recalcitrant folliculitis barbae and pseudofolliculitis barbae with photodynamic therapy

    DEFF Research Database (Denmark)

    Fraes Diernaes, Jon Erik; Bygum, Anette

    2013-01-01

    Folliculitis and pseudofolliculitis barbae typically affects men with curly hair who shave too close. Treatment modalities vary in effectiveness and include improved hair removal methods, topical corticosteroids, topical and oral antibiotics, and retinoids as well as laser surgery. We report...

  20. Route-Clearance Operations

    Science.gov (United States)

    1995-08-01

    Battalion in Alaska. He served as an Observer I Controller at the Joint Readiness Training Center at Fort Polh , LA. and is cur- rently working as the engineer in the JRTC Plans I Exercise Maneuver Control Center. Engineer 61

  1. ¿Existe un Impacto Significativo de la distribución del Canon Minero en las Inversiones Públicas Regionales en el Perú? Metodología de Distribución y Eficiencia del Gasto en Debate

    OpenAIRE

    Vargas Merino, Jorge Alberto

    2015-01-01

    Treballs Finals del Màster Universitari d'Economia, Regulació i Competència als Serveis Públics (ERCSP), Facultat d'Economia i Empresa, Universitat de Barcelona, Curs: 2015-2016, Tutora: Monserrat Termes Rife

  2. Modified gum Arabic hydrogels as matrices for controlled release of curcumin supramolecular complexes; Hidrogeis de goma arabica modificada como matrizes para libertacao controlada de complexos supramoleculares de curcumina

    Energy Technology Data Exchange (ETDEWEB)

    Gerola, Adriana P.; Silva, Danielle C., E-mail: avalente@ci.uc.pt [Department of Chemistry, University of Coimbra, Coimbra (Portugal); Rubira, Adley F.; Muniz, Edvani C. [Universidade Estadual de Maringa (GMPC/UEM), PR (Brazil). Grupo de Materiais Polimericos e Compositos; Jesus, Sandra; Borges, Olga [Faculty of Pharmacy, University of Coimbra, Coimbra (Portugal)

    2015-07-01

    Modified gum Arabic (GA) hydrogels show a pH-responsive behavior making them excellent matrices to be used for oral administration of drugs. Our goal is to study the behavior of those matrices in simulated gastric and intestinal fluids. In this work we will present how the methacrylation degree of GA, by using glycidyl methacrylate, can affect the properties of these hydrogels for controlled release. The drug used in this work is the curcumin (Cur). Cur is associated with numerous pharmacological activities, but their application is limited by the low water solubility. We will present some studies involving the formation of host-guest complexes between Cur and natural cyclodextrins. Both modified GA and hydrogels were characterized by different techniques. The kinetics release of Cur complex-containing modified GA hydrogels was studied to have an insight on the release mechanism and rate constants. Toxicity studies on undifferentiated and differentiated Caco-2 were also carried out. (author)

  3. Taking Care of Your Hair

    Science.gov (United States)

    ... clean so hair oils don't clog your pores. If you're washing your hair every day ... in curly hair. Relaxers containing lye can cause skin irritation and hair breakage. Although "no lye" relaxers ...

  4. The Clementine mission – A 10-year perspective

    Indian Academy of Sciences (India)

    CPT) that measured plasma, total .... Assurance Experiment. The RRELAX consisted of static random access memory (SRAM) ..... schedule, and ground network status – the cur- rent operational factors external to the science mission that may ...

  5. Dissolution of South African Eucalyptus sawdust wood in [Emim][OAc]/Co-solvent mixtures

    CSIR Research Space (South Africa)

    Tywabi, Zikhona

    2017-03-01

    Full Text Available that the surface morphology of the regenerated cellulose samples exhibited a structure that was rather loose, disordered and curly: this was probably due to removal of lignin and decrease in cellulose crystallinity....

  6. Biological and Host Range Studies with Bagous affinis, An Indian Weevil that Destroys Hydrilla Tubers. Aquatic Plant Control Research Program.

    Science.gov (United States)

    1994-12-01

    Fabacae); iris, ornamental Iris sp. (Iridaceae); lily, ornamental Lilium sp. (Liliaceae); banana, Musa X paradisiaca L (Musaceae); curly dock, Rumex ... crispus L. (Polygonaceae); smartweed, Polygonum densiflorum Meissner (Polygonaceae); portulaca, ornamental Portulaca sp. (Portulacaceae). 2 Plant

  7. How hard do mineworkers work? An assessment of workplace stress associated with routine mining activities

    CSIR Research Space (South Africa)

    Schutte, PC

    2012-03-01

    Full Text Available Mining operations are frequently associated with difficult working conditions and high levels of workplace stress. Workplace stress can be defined as the harmful physical and emotional responses that oc-cur when the psychological and...

  8. Maksuameti eksjuht ostis 66000kroonise Picasso

    Index Scriptorium Estoniae

    1999-01-01

    Modernistliku kunsti klassikute näituselt galeriis "Vaal" osteti kaks Picasso keraamilist teost: "Curly Hair Face" omandas 66000 krooni eest Kalev Järvelill ja "Man's Face" 33000 krooni eest ERA-Panga pankrotihaldur Veli Kraavi.

  9. Preparation and in vivo pharmacokinetics of curcumin-loaded PCL-PEG-PCL triblock copolymeric nanoparticles

    Directory of Open Access Journals (Sweden)

    Feng R

    2012-07-01

    Full Text Available Runliang Feng,1,* Zhimei Song,1,* Guangxi Zhai2 1Department of Pharmaceutical Engineering, College of Medicine and Life Science, University of Jinan, Jinan, Shandong Province, 2Department of Pharmaceutics, College of Pharmacy, Shandong University, Jinan, Shandong Province, People's Republic of China*These authors contributed equally to this workBackground: Curcumin (CUR has been linked with antioxidant, anti-inflammatory, antimicrobial, anti amyloid, and antitumor effects, but its application is limited because of its low aqueous solubility and poor oral bioavailability.Methods: To improve its bioavailability and water solubility, we synthesized two series of poly (ε-Caprolactone-poly (ethylene glycol-poly (ε-Caprolactone triblock copolymers by ring-opening polymerization of poly (ethylene glycol and ε-Caprolactone, with stannous 2-ethylhexanoate as the catalyst. Structure of the copolymers was characterized by proton nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and gel permeation chromatography. The nanoparticles (NPs were prepared using a probe-type ultrasonic emulsion and solvent evaporation method. To obtain an optimal delivery system, we explored the effect of the length of the copolymers' hydrophilic and hydrophobic chains on the encapsulation of hydrophobic CUR, performing entrapment efficiency and drug loading evaluations, as well as studying the particle distribution and in vitro release using the direct dispersion method. Finally, study of the in vivo pharmacokinetics of the CUR-loaded NPs was also carried out on selected copolymers in comparison with CUR solution formulations.Results: CUR was encapsulated with 94.3% and 95.5% efficiency in biodegradable nanoparticulate formulations based on NP43 and NP63, respectively. Dynamic laser light scattering and transmission electron microscopy indicated a particle diameter of 55.6 nm and 62.4 nm for NP43 and NP63, respectively. Fourier transform

  10. Studies on Drosophila radiosensitive strains

    International Nuclear Information System (INIS)

    Varentsova, E.P.; Zakharov, I.A.

    1976-01-01

    45 of radiosensitive strains of Drosophila melanogaster were isolated by Curly/Lobe technique after EMS treatment of Livadia population males. The lethality of non-Curly late larvae after gamma-irradiation (4000r) characterized radiosensitivity strains. Most of them exhibited higher frequency of the spontaneous dominant lethals (up to 69%). The males of 6 strains were semi-sterile. 5 of these strains exhibited higher frequency of X-chromosome non-disjunction

  11. Los problemas actuales de la libertad

    Directory of Open Access Journals (Sweden)

    Carlos Llano C.

    2013-11-01

    Full Text Available The article studies three aspects of Freedom: if it exists (an sit in Latin, why it is (cur sit, what it is (quid sit. Psychological Determinism has objected the an sit. While Logical Positivism questions the possibility of knowing the reason (cur sit of Freedom. Finally, the essence of Freedom (quid sit has been obscured by some particulars forms of Freedom (as autonomy and liberation.

  12. Synergistic effects of thymoquinone and curcumin on immune response and anti-viral activity against avian influenza virus (H9N2) in turkeys.

    Science.gov (United States)

    Umar, S; Shah, M A A; Munir, M T; Yaqoob, M; Fiaz, M; Anjum, S; Kaboudi, K; Bouzouaia, M; Younus, M; Nisa, Q; Iqbal, M; Umar, W

    2016-07-01

    The main objective of this study was to determine the possible effects of thymoquinone (TQ) and curcumin (Cur) on immune-response and pathogenesis of H9N2 avian influenza virus (AIV) in turkeys. The experiment was performed on 75 non-vaccinated mixed-sex turkey poults, divided into 5 experimental groups (A, B, C, D, and E) of 15 birds each. Group A was kept as non-infected and a non-treated negative control (ctrl group) while group B was kept as infected and non-treated positive control (H9N2 group). Turkeys in groups A and B received normal commercial feed while turkeys in groups C and D received TQ, and Cur respectively, and group E concurrently received TQ and Cur from d one through the entire experiment period. All groups were challenged intra-nasally with H9N2 AIV (A/chicken/Pakistan/10RS3039-284-48/2010) at the fourth wk of age except group A. Infected turkeys showed clinical signs of different severity, showing the most prominent disease signs in turkeys in group B. All infected turkeys showed positive results for virus shedding; however, the pattern of virus shedding was different, and with turkeys in group B showing more pronounced virus secretion than the turkeys in the other groups receiving different levels of TQ and Cur. Moreover, significantly higher antibody titer against H9N2 AIV in turkeys shows the immunomodulatory nature of TQ and Cur. Similarly, increased cytokine gene expression suggests antiviral behavior of TQ and Cur especially in combination, leading to suppressed pathogenesis of H9N2 viruses. However, reduced virus shedding and enhanced immune responses were more pronounced in those turkeys receiving TQ and Cur concurrently. This study showed that supplements of TQ and Cur in combination would significantly enhance immune responsiveness and suppress pathogenicity of influenza viruses in turkeys. © 2016 Poultry Science Association Inc.

  13. Curcumin improves regulatory T cells in gut-associated lymphoid tissue of colitis mice.

    Science.gov (United States)

    Zhao, Hai-Mei; Xu, Rong; Huang, Xiao-Ying; Cheng, Shao-Min; Huang, Min-Fang; Yue, Hai-Yang; Wang, Xin; Zou, Yong; Lu, Ai-Ping; Liu, Duan-Yong

    2016-06-21

    To explore the probable pathway by which curcumin (Cur) regulates the function of Treg cells by observing the expression of costimulatory molecules of dendritic cells (DCs). Experimental colitis was induced by administering 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution. Forty male C57BL/6 mice were randomly divided into four groups: normal, TNBS + Cur, TNBS + mesalazine (Mes) and TNBS groups. The mice in the TNBS + Cur and TNBS +Mes groups were treated with Cur and Mes, respectively, while those in the TNBS group were treated with physiological saline for 7 d. After treatment, the curative effect of Cur was evaluated by colonic weight, colonic length, weight index of the colon, and histological observation and score. The levels of CD4(+)CD25+Foxp3(+) T cells (Treg cells) and costimulatory molecules of DCs were measured by flow cytometry. Also, related cytokines were analyzed by enzyme-linked immunosorbent assay. Cur alleviated inflammatory injury of the colonic mucosa, decreased colonic weigh and histological score, and restored colonic length. The number of Treg cells was increased, while the secretion of TNF-α, IL-2, IL-6, IL-12 p40, IL-17 and IL-21 and the expression of costimulatory molecules (CD205, CD54 [ICAM-1], TLR4, CD252[OX40 L], CD256 [RANK] and CD254 [RANK L]) of DCs were notably inhibited in colitis mice treated with Cur. Cur potentially modulates activation of DCs to enhance the suppressive functions of Treg cells and promote the recovery of damaged colonic mucosa in inflammatory bowel disease.

  14. Tocotrienol Nanoemulsion Platform of Curcumin Elicit Elevated Apoptosis and Augmentation of Anticancer Efficacy against Breast and Ovarian Carcinomas

    Directory of Open Access Journals (Sweden)

    Nelson Steuber

    2016-10-01

    Full Text Available Vitamin E (VE tocotrienols (T3, recognized for their cancer-specific anti-proliferative and pro-apoptotic activities, have been previously fabricated into bio-active nanoemulsion (NE formulations. Here, our viscosity-adapted δ-T3 NE platform was developed to additionally incorporate curcumin (CUR, which is known for its potent suppression of signaling pathways involved in malignant cell growth, survival and metastasis. Thanks to efficient 70:30 wt % surfactant mix of Lutrol F-127:VE-TPGS, in conjunction with optimal CUR loading, a prototype CUR in δ-T3 NE was successfully prepared. Model CUR/δ-T3 NE demonstrated excellent nano-scale aspects (mean particle size = 261 nm, PDI = 0.27, and ζ-potential = −35 mV, pharmaceutical stability, and controlled release properties. Suitability for systemic administration was also verified via standardized in vitro biocompatibility and hemocompatibility assays. In two human cancer cells (MCF-7 and OVCAR-8, our CUR/δ-T3 NE prominently suppressed constitutive NF-κB activation, and significantly induced apoptosis. Finally, the combined CUR/δ-T3 NE produced superior cytotoxicity profiles, in concentration- and time-dependent manners (p ≤ 0.05, at least three to four folds lower IC50 than in closest CUR control. The strong synergism, estimated in both cultured carcinomas, revealed the augmented therapeutic efficacy of our CUR/δ-T3 NE combined platform, supporting its strong potential towards pharmaceutical development for cancer therapy.

  15. The role of the vascular endothelial growth factor/vascular endothelial growth factor receptors axis mediated angiogenesis in curcumin-loaded nanostructured lipid carriers induced human HepG2 cells apoptosis

    Directory of Open Access Journals (Sweden)

    Fengling Wang

    2015-01-01

    Full Text Available Background: Curcumin (diferuloylmethane, the active constituent of turmeric extract has potent anti-cancer properties have been demonstrated in hepatocellular carcinoma (HCC. However, its underlying molecular mechanism of therapeutic effects remains unclear. Vascular endothelial growth factor (VEGF and its receptors (VEGFRs have crucial roles in tumor angiogenesis. Purpose: The goal of this study was to investigate the role of the VEGF/VEGFRs mediated angiogenesis during the proliferation and apoptosis of human HepG2 hepatoma cell line and the effect of curcumin-loaded nanostructured lipid carriers (Cur-NLC. Materials and Methods: The proliferation of HepG2 cells was determined by methyl thiazolyl tetrazolium after exposure to Cur-NLC and native curcumin. Apoptosis was quantified by flow cytometry with annexin V-fluorescein isothiocyanate and propidium iodide staining. Cellular internalization of Cur-NLC was observed by fluorescent microscope. The level of VEGF was detected by enzyme-linked immunosorbent assay kits. The expression of VEGFRs was quantified by Western blotting. Results: Cur-NLC was more effective in inhibiting the proliferation and enhancing the apoptosis of HepG2 cells than native curcumin. Fluorescent microscope analysis showed that HepG2 cells internalized Cur-NLC more effectively than native curcumin. Furthermore, Cur-NLC down-regulated the level of VEGF and the expression of VEGFR-2, but had a slight effect on VEGFR-1. Conclusion: These results clearly demonstrated that Cur-NLC was more effective in anti-cancer activity than the free form of curcumin. These studies demonstrate for the 1 st time that Cur-NLC exerts an antitumor effect on HepG2 cells by modulating VEGF/VEGFRs signaling pathway.

  16. Curcumin-mediated anti-microbial photodynamic therapy against Candida dubliniensis biofilms.

    Science.gov (United States)

    Sanitá, Paula Volpato; Pavarina, Ana Cláudia; Dovigo, Lívia Nordi; Ribeiro, Ana Paula Dias; Andrade, Mariana Carvalho; Mima, Ewerton Garcia de Oliveira

    2017-11-13

    The purpose of this study was to evaluate the effectiveness of anti-microbial photodynamic therapy (aPDT) mediated by curcumin (Cur) associated with LED light against biofilms of Candida dubliniensis, and further, investigate cellular uptake and drug penetration through the biofilms under confocal laser scanning microscopy (CLSM). Four C. dubliniensis strains were tested: three clinical isolates from HIV-positive patients and one reference strain (CBS 7987). Biofilms were treated with three Cur concentrations (20.0, 30.0, and 40.0 μM). All samples were incubated in the dark for 20 min and exposed to a 5.28 J/cm 2 of LED light fluence. Additional samples of each strain were treated either with Cur or LED light only. Control samples had neither Cur nor light. After aPDT, results were read using the XTT salt reduction method. The data were statistically analyzed by two-way ANOVA followed by Games-Howell post-hoc test (α = 0.05). Confocal laser scanning microscopy was used to verify both the uptake of Cur by yeast cells and its penetration through the biofilm. The results showed that aPDT promoted significant reduction on the metabolism of the biofilm-organized cells of C. dubliniensis. Further, while Cur was rapidly taken up by C. dubliniensis cells, a longer time interval was required to allow Cur penetration into biofilm cells. Based on these results, aPDT associating LED and Cur presents promising potential on fungal control of biofilms of C. dubliniensis.

  17. Design and evaluation of dual CD44 receptor and folate receptor-targeting double-smart pH-response multifunctional nanocarrier

    Science.gov (United States)

    Chen, Daquan; Song, Xiaoyan; Wang, Kaili; Guo, Chunjing; Yu, Yueming; Fan, Huaying; Zhao, Feng

    2017-12-01

    In this article, in order to enhance the bioavailiability and tumor targeting of curcumin (Cur), the oligosaccharides of hyaluronan conjugates, folic acid-oligosaccharides of hyaluronan-acetal-menthone 1,2-glycerol ketal (FA-oHA-Ace-MGK) carried oHA as a ligand to CD44 receptor, double-pH-sensitive Ace-MGK as hydrophobic moieties, and FA as the target of folate receptor. The structure characteristics of this smart response multifunctional dual-targeting nano-sized carrier was measured by fourier-transform infrared (FT-IR) and nuclear magnetic resonance (1H-NMR). Cur, an anticancer drug, was successfully loaded in FA-oHA-Ace-MGK micelles by self-assembly. The measurement results of transmission electron microscopy (TEM) presented that the Cur-loaded micelles were spherical in shape with the average size of 166.3 ± 2.12 nm and zeta potential - 30.07 mV. Much more encapsulated Cur could be released at mildly acidic environments than at pH 7.4, from the Cur-FA-oHA-Ace-MGK micelles. Cytotoxicity assay indicated that non-Cur loaded micelles mostly had no cytotoxicity to MCF-7 cells and A549 cells, and Cur-loaded micelles had significantly lower survival rate than Cur suspension in the same concentration, which proved that the drug-loaded micelles can effectively inhibit tumor cell growth. The targeting of CD44 receptors and folate receptors was proved in vitro cellular uptake assay. These results showed the promising potential of FA-oHA-Ace-MGK as an effective nano-sized carrier for anti-tumor drug delivery.

  18. The Effectiveness of Natural Diarylheptanoids against Trypanosoma cruzi: Cytotoxicity, Ultrastructural Alterations and Molecular Modeling Studies.

    Directory of Open Access Journals (Sweden)

    Vitor Sueth-Santiago

    Full Text Available Curcumin (CUR is the major constituent of the rhizomes of Curcuma longa and has been widely investigated for its chemotherapeutic properties. The well-known activity of CUR against Leishmania sp., Trypanosoma brucei and Plasmodium falciparum led us to investigate its activity against Trypanosoma cruzi. In this work, we tested the cytotoxic effects of CUR and other natural curcuminoids on different forms of T. cruzi, as well as the ultrastructural changes induced in epimastigote form of the parasite. CUR was verified as the curcuminoid with more significant trypanocidal properties (IC50 10.13 μM on epimastigotes. Demethoxycurcumin (DMC was equipotent to CUR (IC50 11.07 μM, but bisdemethoxycurcumin (BDMC was less active (IC50 45.33 μM and cyclocurcumin (CC was inactive. In the experiment with infected murine peritoneal macrophages all diarylheptanoids were more active than the control in the inhibition of the trypomastigotes release. The electron microscopy images showed ultrastructural changes associated with the cytoskeleton of the parasite, indicating tubulin as possible target of CUR in T. cruzi. The results obtained by flow cytometry analysis of DNA content of the parasites treated with natural curcuminoids suggested a mechanism of action on microtubules related to the paclitaxel`s mode of action. To better understand the mechanism of action highlighted by electron microscopy and flow cytometry experiments we performed the molecular docking of natural curcuminoids on tubulin of T. cruzi in a homology model and the results obtained showed that the observed interactions are in accordance with the IC50 values found, since there CUR and DMC perform similar interactions at the binding site on tubulin while BDMC do not realize a hydrogen bond with Lys163 residue due to the absence of methoxyl groups. These results indicate that trypanocidal properties of CUR may be related to the cytoskeletal alterations.

  19. Curcumin improves regulatory T cells in gut-associated lymphoid tissue of colitis mice

    Science.gov (United States)

    Zhao, Hai-Mei; Xu, Rong; Huang, Xiao-Ying; Cheng, Shao-Min; Huang, Min-Fang; Yue, Hai-Yang; Wang, Xin; Zou, Yong; Lu, Ai-Ping; Liu, Duan-Yong

    2016-01-01

    AIM: To explore the probable pathway by which curcumin (Cur) regulates the function of Treg cells by observing the expression of costimulatory molecules of dendritic cells (DCs). METHODS: Experimental colitis was induced by administering 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution. Forty male C57BL/6 mice were randomly divided into four groups: normal, TNBS + Cur, TNBS + mesalazine (Mes) and TNBS groups. The mice in the TNBS + Cur and TNBS +Mes groups were treated with Cur and Mes, respectively, while those in the TNBS group were treated with physiological saline for 7 d. After treatment, the curative effect of Cur was evaluated by colonic weight, colonic length, weight index of the colon, and histological observation and score. The levels of CD4+CD25+Foxp3+ T cells (Treg cells) and costimulatory molecules of DCs were measured by flow cytometry. Also, related cytokines were analyzed by enzyme-linked immunosorbent assay. RESULTS: Cur alleviated inflammatory injury of the colonic mucosa, decreased colonic weigh and histological score, and restored colonic length. The number of Treg cells was increased, while the secretion of TNF-α, IL-2, IL-6, IL-12 p40, IL-17 and IL-21 and the expression of costimulatory molecules (CD205, CD54 [ICAM-1], TLR4, CD252[OX40 L], CD256 [RANK] and CD254 [RANK L]) of DCs were notably inhibited in colitis mice treated with Cur. CONCLUSION: Cur potentially modulates activation of DCs to enhance the suppressive functions of Treg cells and promote the recovery of damaged colonic mucosa in inflammatory bowel disease. PMID:27340353

  20. Effects of Micro-environmental pH of Liposome on Chemical Stability of Loaded Drug.

    Science.gov (United States)

    Shao, Xiao-Ru; Wei, Xue-Qin; Zhang, Shu; Fu, Na; Lin, Yun-Feng; Cai, Xiao-Xiao; Peng, Qiang

    2017-08-23

    Liposome is a promising carrier system for delivering bioactive molecules. However, the successful delivery of pH-sensitive molecules is still limited by the intrinsic instability of payloads in physiological environment. Herein, we developed a special liposome system that possesses an acidic micro-environment in the internal aqueous chamber to improve the chemical stability of pH-sensitive payloads. Curcumin-loaded liposomes (Cur-LPs) with varied internal pH values (pH 2.5, 5.0, or 7.4) were prepared. These Cur-LPs have similar particle size of 300 nm, comparable physical stabilities and analogous in vitro release profiles. Interestingly, the chemical stability of liposomal curcumin in 50% fetal bovine serum and its anticancer efficacy in vitro are both micro-environmental pH-dependent (Cur-LP-2.5 > Cur-LP-5.0 > Cur-LP-7.4). This serum stability still has space to be further enhanced to improve the applicability of Cur-LP. In conclusion, creating an acidic micro-environment in the internal chamber of liposome is feasible and efficient to improve the chemical stability of pH-sensitive payloads.

  1. A comparative study of the spectral, fluorometric properties and photostability of natural curcumin, iron- and boron- complexed curcumin

    Science.gov (United States)

    Mohammed, Fatima; Rashid-Doubell, Fiza; Cassidy, Seamas; Henari, Fryad

    2017-08-01

    Curcumin is a yellow phenolic compound with a wide range of reported biological effects. However, two main obstacles hinder the use of curcumin therapeutically, namely its poor bioavailability and photostability. We have synthesized two curcumin complexes, the first a boron curcumin complex (B-Cur2) and the second an iron (Fe-Cur3) complex of curcumin. Both derivatives showed high fluorescence efficiency (quantum yield) and greater photostability in solution. The improved photostability could be attributed to the coordination structures and the removal of β-diketone group from curcumin. The fluorescence and ultra violet/visible absorption spectra of curcumin, B-Cur2 and Fe-Cur3 all have a similar spectral pattern when dissolved in the same organic solvent. However, a shift towards a lower wavelength was observed when moving from polar to non-polar solvents, possibly due to differences in solvent polarity. A plot of Stokes' shift vs the orientation polarity parameter (Δf) or vs the solvent polarity parameter (ET 30) showed an improved correlation between the solvent polarity parameter than with the orientation polarity parameter and indicating that the red shift observed could be due to hydrogen-bonding between the solvent molecules. A similar association was obtained when Stokes' shift was replaced by maximum synchronous fluorescence. Both B-Cur2 and Fe-Cur3 had larger quantum yields than curcumin, suggesting they may be good candidates for medical imaging and in vitro studies.

  2. Effects of early morning care, named "Comfort upon Rising" care, on postoperative orthopedic ambulation and morning activity.

    Science.gov (United States)

    Ohashi, Kumiko

    2014-10-01

    To examine the effects of "Comfort upon Rising" care (CUR) as a new type of early morning care. This was a quasi-experimental study using a convenience sampling and non-random assignment of 80 orthopedic postoperative inpatients needing ambulation assistance. The intervention group of 40 inpatients was given CUR designed to support the patients' needs. The control group of 40 inpatients was given conventional early morning care (CMC). Floor nurses conducted either CMC or CUR for subjects beginning the morning after surgery and continuing for 3 days. Mood was measured by the Wake-up Questionnaire (question I) and Profile of Mood States (POMS). Morning activities, which were daily living activities, breakfast behaviors including breakfast intake rate, were measured by Wake-up Questionnaire (question IV) and observation. Completing the study were 36 patients from the control group and 36 patients from the intervention group. Mood and activities in the morning were improved in the CUR group. CUR is a patient-centered care based on patients' individual needs and promotes self-care. CUR is more effective than CMC in promoting natural recovery after an operation. © 2013 The Author. Japan Journal of Nursing Science © 2013 Japan Academy of Nursing Science.

  3. Effects of curcumin on growth of human cervical cancer xenograft in nude mice and underlying mechanism

    Directory of Open Access Journals (Sweden)

    Aixue LIU

    Full Text Available Abstract The present study investigated the effects of curcumin (Cur on growth of human cervical cancer xenograft in nude mice and underlying mechanism. The nude mice modeled with human cervical cancer HeLa cell xenograft were treated with normal saline (control, 3 mg/kg Cisplatin, 50, 100 and 200 mg/kg Cur, respectively. The animal body weight and growth of tumor were measured. The expressions of Bax, Bcl-2, p53, p21, HIF-1α, VEGF and MIF protein in tumor tissue were determined. Results showed that, after treatment for 20 days, the tumor mass and tumor volume in 100 and 200 mg/kg Cur group were significantly lower than control group (P < 0.05. The expressions of Bax, p53 and p21 protein in tumor tissue in 200 mg/kg Cur group were significantly higher than control group (P < 0.05, and the expressions of Bcl-2, HIF-1α, VEGF and MIF protein in tumor tissue in 200 mg/kg Cur group were significantly lower than control group (P < 0.05. Cur can inhibit the growth of HeLa cell xenograft in nude mice. The possible mechanism may be related to its up-regulation of Bax, p53 and p21 protein expression in tumor tissue, and down-regulation of Bcl-2, HIF-1α, VEGF and MIF protein expression.

  4. Nanocarrier with self-antioxidative property for stabilizing and delivering ascorbyl palmitate into skin.

    Science.gov (United States)

    Janesirisakule, Sirinapa; Sinthusake, Tarit; Wanichwecharungruang, Supason

    2013-08-01

    The concept of a nanocarrier with a self-antioxidative property to deliver and stabilize a labile drug while at the same time providing a free radical scavenging activity is demonstrated. Curcumin was grafted onto a poly(vinyl alcohol) [PV(OH)] chain, and the nanocarriers fabricated from the obtained curcumin-grafted PV(OH) polymer [CUR-PV(OH)] showed a good free radical scavenging activity. Ascorbyl palmitate (AP) could be effectively loaded into the CUR-PV(OH) at 29% by weight. The CUR-PV(OH)-encapsulated AP was 77% more stable than the free (unencapsulated) AP, and 47% more stable than AP encapsulated in the control nanocarrier with no antioxidative property [cinnamoyl-grafted PV(OH); CIN-PV(OH)]. Although coencapsulation of curcumin and AP into CIN-PV(OH) showed some improvement on the AP stability, AP was more stable when encapsulated in CUR-PV(OH). Compared with the free AP, encapsulated AP within the CUR-PV(OH) nanocarriers showed not only a better penetration into pig skin dermis via hair follicle pathway followed by the release and diffusion of the AP, but also a greater AP stability after skin application. Although a proof of principle is shown for CUR-PV(OH) and AP, it is likely that other carriers of the same principal could be designed and applied to different oxidation-sensitive drugs. Copyright © 2013 Wiley Periodicals, Inc.

  5. Greening Industrial Production through Waste Recovery: "Comprehensive Utilization of Resources" in China.

    Science.gov (United States)

    Zhu, Junming; Chertow, Marian R

    2016-03-01

    Using nonhazardous wastes as inputs to production creates environmental benefits by avoiding disposal impacts, mitigating manufacturing impacts, and conserving virgin resources. China has incentivized reuse since the 1980s through the "Comprehensive Utilization of Resources (CUR)" policy. To test whether and to what extent environmental benefits are generated, 862 instances in Jiangsu, China are analyzed, representing eight industrial sectors and 25 products that qualified for tax relief through CUR. Benefits are determined by comparing life cycle inventories for the same product from baseline and CUR-certified production, adjusted for any difference in the use phase. More than 50 million tonnes of solid wastes were reused, equivalent to 51% of the provincial industrial total. Benefits included reduction of 161 petajoules of energy, 23 million tonnes of CO2 equivalent, 75 000 tonnes of SO2 equivalent, 33 000 tonnes of NOX, and 28 000 tonnes of PM10 equivalent, which were 2.5%-7.3% of the provincial industrial consumption and emissions. The benefits vary substantially across industries, among products within the same industry, and when comparing alternative reuse processes for the same waste. This first assessment of CUR results shows that CUR has established a firm foundation for a circular economy, but also suggest additional opportunities to refine incentives under CUR to increase environmental gain.

  6. Structural characterization, formation mechanism and stability of curcumin in zein-lecithin composite nanoparticles fabricated by antisolvent co-precipitation.

    Science.gov (United States)

    Dai, Lei; Sun, Cuixia; Li, Ruirui; Mao, Like; Liu, Fuguo; Gao, Yanxiang

    2017-12-15

    Curcumin (Cur) exhibits a range of bioactive properties, but its application is restrained due to its poor water solubility and sensitivity to environmental stresses. In this study, zein-lecithin composite nanoparticles were fabricated by antisolvent co-precipitation technique for delivery of Cur. The result showed that the encapsulation efficiency of Cur was significantly enhanced from 42.03% in zein nanoparticles to 99.83% in zein-lecithin composite nanoparticles. The Cur entrapped in the nanoparticles was in an amorphous state confirmed by differential scanning calorimetry and X-ray diffraction. Fourier transform infrared analysis revealed that hydrogen bonding, electrostatic interaction and hydrophobic attraction were the main interactions among zein, lecithin, and Cur. Compared with single zein and lecithin nanoparticles, zein-lecithin composite nanoparticles significantly improved the stability of Cur against thermal treatment, UV irradiation and high ionic strength. Therefore, zein-lecithin composite nanoparticles could be a potential delivery system for water-insoluble bioactive compounds with enhanced encapsulation efficiency and chemical stability. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Facile and large-scale synthesis of curcumin/PVA hydrogel: effectively kill bacteria and accelerate cutaneous wound healing in the rat.

    Science.gov (United States)

    He, Fei; Jiao, Hongjing; Tian, Yu; Zhao, Libo; Liao, Xiaozhu; Fan, Zengjie; Liu, Bin

    2018-03-01

    The complicated synthesis procedure and limited preparation size of hydrogel inhibit its clinical application. Therefore, a facile preparation method for large-size hydrogel is required. In this study, a series of curcumin (Cur)/polyvinyl alcohol (PVA) hydrogel in a large size with different Cur concentrations is prepared by a facile physical-chemical crosslinking. The physicochemical properties, antibacterial performance and accelerating wound healing ability are evaluated with the aim of attaining a novel and effective wound dressing. The results show that the as-prepared hydrogel with the optimal Cur to PVA volume ratio of 1:5 (20% Cur/PVA) exhibits the best antibacterial abilities to E. coli (85.6%) and S. aureus (97%) than other hydrogels. When the volume ratio of Cur to PVA is 1:10 (10% Cur/PVA), the hydrogel can significantly accelerate the wound healing in rats, and successfully reconstruct intact and thickened epidermis during 14 day of healing of impaired wounds after histological examination. In one word, the present approach can shed new light on designing new type of hydrogels with promising applications in wound dressing.

  8. A Novel Iris Segmentation Scheme

    Directory of Open Access Journals (Sweden)

    Chen-Chung Liu

    2014-01-01

    Full Text Available One of the key steps in the iris recognition system is the accurate iris segmentation from its surrounding noises including pupil, sclera, eyelashes, and eyebrows of a captured eye-image. This paper presents a novel iris segmentation scheme which utilizes the orientation matching transform to outline the outer and inner iris boundaries initially. It then employs Delogne-Kåsa circle fitting (instead of the traditional Hough transform to further eliminate the outlier points to extract a more precise iris area from an eye-image. In the extracted iris region, the proposed scheme further utilizes the differences in the intensity and positional characteristics of the iris, eyelid, and eyelashes to detect and delete these noises. The scheme is then applied on iris image database, UBIRIS.v1. The experimental results show that the presented scheme provides a more effective and efficient iris segmentation than other conventional methods.

  9. On-line analysis of AEP and EEG for monitoring depth of anaesthesia

    DEFF Research Database (Denmark)

    Capitanio, L; Jensen, E W; Filligoi, G C

    1997-01-01

    the left hemisphere and analysed by FFT to 1 sec epochs and the spectral edge frequency was calculated. Both the changes in ARX extracted AEP and the spectral edge frequency of the EEG correlated well with the time interval between propofol induction and onset of anaesthesia measured by clinical signs (i.......e., cessation of eye-lash reflex). The MTA extracted AEP was significantly slower in tracing the transition from consciousness to unconsciousness....

  10. [Ophthalmological manifestations of Cornelia de Lange syndrome: Case report and review of the literature].

    Science.gov (United States)

    Avgitidou, G; Cursiefen, C; Heindl, L M

    2015-05-01

    A 2-year-old boy suffering from Cornelia de Lange syndrome, presented with mucopurulent ocular discharge and epiphora since birth. Irrigation and probing of the nasolacrimal system revealed and successfully treated bilateral nasolacrimal duct obstructions. Cornelia de Lange syndrome is characterized not only by typical facial features, visceral and urogenital anomalies but also by ophthalmological manifestations in 99% of cases. The most common ophthalmological disorders are synophrys, blepharitis, epiphora, hypertrichosis of the eyebrows and eyelashes, myopia, ptosis and nasolacrimal duct obstruction.

  11. Hutchinson-Gilford progeria syndrome

    Directory of Open Access Journals (Sweden)

    Agarwal Uma

    2010-01-01

    Full Text Available Progeria is a rare genetic disorder characterized by premature aging, involving the skin, bones, heart, and blood vessels. We report a 4-year-old boy who presented with clinical manifestations of progeria. He had characteristic facies, prominent eyes, scalp and leg veins, senile look, loss of scalp hair, eyebrows and eyelashes, stunted growth, and sclerodermatous changes. The present case is reported due to its rarity.

  12. Nickel ferrule applicators: a source of nickel exposure in children.

    Science.gov (United States)

    Jacob, Sharon E; Silverberg, Jonathan I; Rizk, Christopher; Silverberg, Nanette

    2015-01-01

    Eye makeup has been investigated for nickel content and found to have no direct association with nickel allergy and cosmetic dermatitis. However, the tools used (e.g., eyelash curlers, hairdressing scissors, hair curlers, and eye shadow and makeup applicators) may be sources. Nickel is ubiquitous and a wide range of sources have been reported, and makeup applicators (ferrules) now join the list. © 2015 Wiley Periodicals, Inc.

  13. Eye cosmetics.

    Science.gov (United States)

    Draelos, Z K

    1991-01-01

    Eye cosmetics are useful to highlight and emphasize the eyes. Currently available eye cosmetics include eye shadows, eye shadow setting creams, under-eye concealers, eye-liners, mascaras, artificial eyelashes, and eyebrow pencils. Special care must be taken when patients with sensitive skin or contact lens wearers select eye cosmetics. Eye cosmetics may also be the cause of either irritant or allergic contact dermatitis, which are two causes of the upper-eyelid dermatitis syndrome.

  14. Beard reconstruction using hair grafts in a free flap.

    Science.gov (United States)

    Levesque, Andre Y; Schalet, Benjamin J; Dolph, James L

    2011-01-01

    Hair transplantation using micrografts or minigrafts is a standard procedure used for hair restoration in androgenic, burn scar and cicatricial alopecia. These grafts have also been used to reconstruct the eyebrow, eyelash, mustache, beard and pubic escutcheon. A patient who underwent successful micrograft and minigraft hair transplantation into a free osteocutaneous mandibular flap reconstruction is presented. The patient was very satisfied with his reconstruction, and the hair transplants provided excellent camouflage for the flap.

  15. Beard reconstruction using hair grafts in a free flap

    OpenAIRE

    Levesque, Andre Y; Schalet, Benjamin J; Dolph, James L

    2011-01-01

    Hair transplantation using micrografts or minigrafts is a standard procedure used for hair restoration in androgenic, burn scar and cicatricial alopecia. These grafts have also been used to reconstruct the eyebrow, eyelash, mustache, beard and pubic escutcheon. A patient who underwent successful micrograft and minigraft hair transplantation into a free osteocutaneous mandibular flap reconstruction is presented. The patient was very satisfied with his reconstruction, and the hair transplants p...

  16. Investigating the effects of metabolic dysregulation on hair follicles: a comparison of HIV-infected women with and without central lipohypertrophy.

    Science.gov (United States)

    Mirmirani, Paradi; Maurer, Toby; Cohen, Mardge; D'Souza, Gypsymber; Karim, Roksana; Plankey, Michael; Robison, Esther; Sharma, Anjali; Tien, Phyllis C; Hessol, Nancy A

    2014-10-01

    Normal lipid metabolism and functioning of the peroxisome proliferator-activated receptor gamma (PPAR-gamma) in the sebaceous gland is critical to maintaining a normal hair follicle. Human immunodeficiency virus (HIV) infection affects lipid metabolism; some have hypothesized a link between PPAR-gamma function and lipodystrophy in HIV infection. Our objective was to determine whether lipodystrophy is associated with altered hair characteristics in HIV-infected women from the Women's Interagency HIV Study. Hair characteristics and scalp inflammation were assessed by an interviewer-administered questionnaire. Central lipohypertrophy and peripheral lipoatrophy were defined by self-report of moderate to severe fat gain in central body sites and fat loss in peripheral body sites, respectively confirmed by clinical examination. Additional covariates considered in the analyses included demographics, behavioral characteristics, medical history, and HIV-related factors. There were 1037 women with data on all study variables; 76 women reported central lipohypertrophy, while only four women reported lipoatrophy. Women with central lipohypertrophy were more likely to be older, had a self-reported history of injection drug use, statin medication use, diabetes, elevated cholesterol, and have self-reported less hair and shorter eyelashes. After adjustment for age, central lipohypertrophy was associated with shorter eyelashes (OR 2.3; 95% CI 1.4-3.8). Central lipohypertrophy was not associated with change in scalp hair texture or scalp inflammation in this cohort. Rather, we found an association between central lipohypertrophy and shorter eyelash length. This finding may be explained by an influence of prostaglandin E2 mediators on eyelash follicles. © 2013 The International Society of Dermatology.

  17. Ichthyosis follicularis with alopecia and photophobia (IFAP syndrome

    Directory of Open Access Journals (Sweden)

    Rai Vandana

    2006-01-01

    Full Text Available A 12-year-old boy born of a nonconsanguineous marriage presented with dry rough skin and photophobia since birth. His growth and developmental milestones were normal and there was no history of any neurological problem, hearing deficit or scarring around the hair follicles. Cutaneous examination revealed diffuse thinning of scalp hair with loss of eyebrows and eyelashes and a sandpapery texture of the skin all over the body, suggestive of ichthyosis follicularis with alopecia and photophobia syndrome.

  18. Determination of the minor and trace elements in Biriniwa's tin pyrite and ornamental lead/zinc ore using neutron activation analysis

    OpenAIRE

    A.O. Adebayo; H.O. Ogunsuyi; M.O.A. Oladipo; O.O. Ajayi

    2002-01-01

    Preliminary results of analysis of two common decorative/ornamental minerals analysed for minor and trace elements with the neutron activation analysis technique are discussed. The samples of interest were the Biriniwa tin pyrite, which the local indigenous used to paint their huts and the ornamental lead which women use to adorn their eyelashes nation-wide. These samples were irradiated along the certified reference sample, CANMET-BLI, with thermal neutron at the Julich Reactor Centre, Julic...

  19. Trichiasis surgery: a patient-based approach

    Directory of Open Access Journals (Sweden)

    Wondu Alemayehu

    2010-12-01

    Full Text Available Trachoma is the leading infectious cause of blindness worldwide. Corneal scarring, which causes trachoma related blindness, occurs when the upper eyelashes are turned inward and rub on the eye (cornea. This is called trichiasis, and if the lid margin turns inward, the term entropion is used. Currently, there are an estimated 8.2 million people with trichiasis and 3.1 million people are blind from trachoma.

  20. Cyclic hexapeptide-conjugated nanoparticles enhance curcumin delivery to glioma tumor cells and tissue

    Directory of Open Access Journals (Sweden)

    Zhang X

    2017-08-01

    Full Text Available Xuemei Zhang,1–3 Xuejuan Li,1,4 Hongchen Hua,1 Aiping Wang,1 Wanhui Liu,1–3 Youxin Li,1–3 Fenghua Fu,1–3 Yanan Shi,5 Kaoxiang Sun1 1School of Pharmacy, Yantai University, Yantai, Shandong Province, People’s Republic of China; 2State Key Laboratory of Long-acting and Targeting Drug Delivery System, Yantai, Shandong Province, People’s Republic of China; 3Luye Pharmaceutical Co., Ltd., Shandong Province, People’s Republic of China; 4National Engineering and Technology Research Center of Chirality Pharmaceutical, Lunan Pharmaceutical Group Co., Ltd., Shandong Province, People’s Republic of China; 5School of Pharmacy, Binzhou Medical University, Shandong Province, People’s Republic of China Abstract: Glioma has one of the highest mortality rates among primary brain tumors. The clinical treatment for glioma is very difficult due to its infiltration and specific growth locations. To achieve improved drug delivery to a brain tumor, we report the preparation and in vitro and in vivo evaluation of curcumin nanoparticles (Cur-NPs. The cyclic hexapeptide c(RGDf(N-meVK-C (cHP has increased affinity for cells that overexpress integrins and was designed to target Cur-NPs to tumors. Functional polyethyleneglycol-modified poly(D,L-lactide-co-glycolide (PEG-PLGA conjugated to cHP was synthesized, and targeted Cur-NPs were prepared using a self-assembly nanoprecipitation process. The physicochemical properties and the in vitro cytotoxicity, accuracy, and penetration capabilities of Cur-NPs targeting cells with high levels of integrin expression were investigated. The in vivo targeting and penetration capabilities of the NPs were also evaluated against glioma in rats using in vivo imaging equipment. The results showed that the in vitro cytotoxicity of the targeted cHP-modified curcumin nanoparticles (cHP/Cur-NPs was higher than that of either free curcumin or non-targeted Cur-NPs due to the superior ability of the cHP/Cur-NPs to target tumor cells

  1. Cardioprotective actions of curcumin on the pathogenic NFAT/COX-2/prostaglandin E2pathway induced during Trypanosoma cruzi infection.

    Science.gov (United States)

    Hernández, Matías; Wicz, Susana; Corral, Ricardo S

    2016-11-15

    Diverse cardiovascular signaling routes have been considered critical for Chagas cardiomyopathy caused by the protozoan parasite Trypanosoma cruzi. Along this line, T. cruzi infection and endothelin-1 (ET-1) have been shown to cooperatively activate the Ca 2+ /NFAT cascade in cardiomyocytes, leading to cyclooxygenase type 2 (COX-2) induction and increased release of prostanoids and prohypertrophic peptides. To determine whether the well-known cardioprotective and anti-inflammatory effects of curcumin (Cur) could be helpful to interfere with this key machinery for pathogenesis of Chagas myocarditis. Cur treatment was evaluated through in vivo studies using a murine model of acute T. cruzi infection and in vitro experiments using ET-1-stimulated and parasite-infected mouse cardiomyocytes. Cur-treated and untreated infected mice were followed-up to estimate survival postinfection and heart tissues from both groups were analyzed for inflammatory infiltration by histopathology, whereas parasite load, induction of arachidonic acid pathway and natriuretic peptide expression were determined by real-time PCR. Molecular analysis of Cur myocardial targets included intracellular calcium measurement, NFAT and COX-2 induction in transfected cells, and assessment of NFAT, COX-2 and microsomal prostaglandin E synthase-1 (mPGES-1) levels by immunoblotting, prostaglandin E 2 (PGE 2 ) by ELISA, b-type natriuretic peptide (BNP) by real-time PCR, and PGE 2 /EP4 receptor/BNP interaction by transwell experiments. Cur treatment of acute Chagas mice enhanced survival and proved to hinder relevant inflammatory processes in the heart, including leukocyte recruitment, activation of the eicosanoid pathway and BNP overexpression, without modifying parasite burden in the organ. Cur was capable of blocking Ca 2+ -dependent NFATc1 transcriptional activity, COX-2 and mPGES-1 induction, and subsequent PGE 2 production in ET-1-stimulated and parasite-infected cardiomyocytes. Furthermore, the decline

  2. Escherichia coli O157:H7 Acid Sensitivity Correlates with Flocculation Phenotype during Nutrient Limitation

    Directory of Open Access Journals (Sweden)

    Kathryn L. Kay

    2017-07-01

    Full Text Available Shiga toxin producing Escherichia coli (STEC strains vary in acid resistance; however, little is known about the underlying mechanisms that result in strain specific differences. Among 25 STEC O157:H7 strains tested, 7 strains flocculated when grown statically for 18 h in minimal salts medium at 37°C, while 18 strains did not. Interestingly, the flocculation phenotype (cells came out of suspension was found to correlate with degree of acid sensitivity in an assay with 400 mM acetic acid solution at pH 3.3 targeting acidified foods. Strains exhibiting flocculation were more acid sensitive and were designated FAS, for flocculation acid sensitive, while the acid resistant strain designated PAR for planktonic acid resistant. Flocculation was not observed for any strains during growth in complex medium (Luria Bertani broth. STEC strains B201 and B241 were chosen as representative FAS (2.4 log reduction and PAR (0.15 log reduction strains, respectively, due to differences in acid resistance and flocculation phenotype. Results from electron microscopy showed evidence of fimbriae production in B201, whereas fimbriae were not observed in B241.Curli fimbriae production was identified through plating on Congo red differential medium, and all FAS strains showed curli fimbriae production. Surprisingly, 5 PAR strains also had evidence of curli production. Transcriptomic and targeted gene expression data for B201 and B241indicated that csg and hde (curli and acid induced chaperone genes, respectively expression positively correlated with the phenotypic differences observed for these strains. These data suggest that FAS strains grown in minimal medium express curli, resulting in a flocculation phenotype. This may be regulated by GcvB, which positively regulates curli fimbriae production and represses acid chaperone proteins. RpoS and other regulatory mechanisms may impact curli fimbriae production, as well. These findings may help elucidate mechanisms

  3. Transport of curcumin derivatives in Caco-2 cell monolayers.

    Science.gov (United States)

    Zeng, Zhen; Shen, Zhe L; Zhai, Shuo; Xu, Jia L; Liang, Hui; Shen, Qin; Li, Qing Y

    2017-08-01

    Curcumin (Cur) is a strong natural antioxidant, who can prevent multiple diseases such as anti-cancer, anti-inflammatory, have a resistance to alzheimer's disease and various malignant diseases. But it has poor oral bioavailability due to its poor aqueous solubility, as well as instability. While its novel derivatives (CB and FE), showed better anti-tumor activity, better anti-oxidant activity and better stability than the original drug (Cur). The aim of this study was to study the intestinal transport of Cur, CB and FE using an in vitro Caco-2 cell monolayer model. The results showed that Cur had a lower permeability coefficient (1.13×10 -6 ±0.11×10 -6 cm/s) for apical-to-basolated (AP-BL) transport at 25μM, while the transport rate for AP to BL flux of CB (3.18×10 -6 ±0.31×10 -6 cm/s) and FE (5.28×10 -6 ±0.83×10 -6 cm/s) were significantly greater than that of Cur. The efflux ratio (ER) value at the concentration of 25μM was 1.31 for Cur, 1.26 for CB and 1.33 for FE, suggesting there was no active efflux involved in the translocation across the Caco-2 cell monolayers for the three compounds. Furthermore, the transport flux of CB and FE was in a concentration dependent manner, suggesting the intestinal transport mechanism in them was passive transport. In summary, the results demonstrated that both the intestinal permeability of CB and FE across Caco-2 cell monolayers was significantly improved compare to Cur. Thus they might show a higher oral bioavailability in vivo, and show the potential application in clinic or nutraceutical. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. A comparative study of dietary curcumin, nanocurcumin, and other classical amyloid-binding dyes for labeling and imaging of amyloid plaques in brain tissue of 5×-familial Alzheimer's disease mice.

    Science.gov (United States)

    Maiti, Panchanan; Hall, Tia C; Paladugu, Leela; Kolli, Nivya; Learman, Cameron; Rossignol, Julien; Dunbar, Gary L

    2016-11-01

    Deposition of amyloid beta protein (Aβ) is a key component in the pathogenesis of Alzheimer's disease (AD). As an anti-amyloid natural polyphenol, curcumin (Cur) has been used as a therapy for AD. Its fluorescent activity, preferential binding to Aβ, as well as structural similarities with other traditional amyloid-binding dyes, make it a promising candidate for labeling and imaging of Aβ plaques in vivo. The present study was designed to test whether dietary Cur and nanocurcumin (NC) provide more sensitivity for labeling and imaging of Aβ plaques in brain tissues from the 5×-familial AD (5×FAD) mice than the classical Aβ-binding dyes, such as Congo red and Thioflavin-S. These comparisons were made in postmortem brain tissues from the 5×FAD mice. We observed that Cur and NC labeled Aβ plaques to the same degree as Aβ-specific antibody and to a greater extent than those of the classical amyloid-binding dyes. Cur and NC also labeled Aβ plaques in 5×FAD brain tissues when injected intraperitoneally. Nanomolar concentrations of Cur or NC are sufficient for labeling and imaging of Aβ plaques in 5×FAD brain tissue. Cur and NC also labeled different types of Aβ plaques, including core, neuritic, diffuse, and burned-out, to a greater degree than other amyloid-binding dyes. Therefore, Cur and or NC can be used as an alternative to Aβ-specific antibody for labeling and imaging of Aβ plaques ex vivo and in vivo. It can provide an easy and inexpensive means of detecting Aβ-plaque load in postmortem brain tissue of animal models of AD after anti-amyloid therapy.

  5. Characterization of the Biosynthetic Genes for 10,11-Dehydrocurvularin, a Heat Shock Response-Modulating Anticancer Fungal Polyketide from Aspergillus terreus

    Science.gov (United States)

    Xu, Yuquan; Espinosa-Artiles, Patricia; Schubert, Vivien; Xu, Ya-ming; Zhang, Wei; Lin, Min; Gunatilaka, A. A. Leslie; Süssmuth, Roderich

    2013-01-01

    10,11-Dehydrocurvularin is a prevalent fungal phytotoxin with heat shock response and immune-modulatory activities. It features a dihydroxyphenylacetic acid lactone polyketide framework with structural similarities to resorcylic acid lactones like radicicol or zearalenone. A genomic locus was identified from the dehydrocurvularin producer strain Aspergillus terreus AH-02-30-F7 to reveal genes encoding a pair of iterative polyketide synthases (A. terreus CURS1 [AtCURS1] and AtCURS2) that are predicted to collaborate in the biosynthesis of 10,11-dehydrocurvularin. Additional genes in this locus encode putative proteins that may be involved in the export of the compound from the cell and in the transcriptional regulation of the cluster. 10,11-Dehydrocurvularin biosynthesis was reconstituted in Saccharomyces cerevisiae by heterologous expression of the polyketide synthases. Bioinformatic analysis of the highly reducing polyketide synthase AtCURS1 and the nonreducing polyketide synthase AtCURS2 highlights crucial biosynthetic programming differences compared to similar synthases involved in resorcylic acid lactone biosynthesis. These differences lead to the synthesis of a predicted tetraketide starter unit that forms part of the 12-membered lactone ring of dehydrocurvularin, as opposed to the penta- or hexaketide starters in the 14-membered rings of resorcylic acid lactones. Tetraketide N-acetylcysteamine thioester analogues of the starter unit were shown to support the biosynthesis of dehydrocurvularin and its analogues, with yeast expressing AtCURS2 alone. Differential programming of the product template domain of the nonreducing polyketide synthase AtCURS2 results in an aldol condensation with a different regiospecificity than that of resorcylic acid lactones, yielding the dihydroxyphenylacetic acid scaffold characterized by an S-type cyclization pattern atypical for fungal polyketides. PMID:23335766

  6. Aluminium-Induced Oxidative Stress, Apoptosis and Alterations in Testicular Tissue and Sperm Quality in Wistar Rats: Ameliorative Effects of Curcumin

    Directory of Open Access Journals (Sweden)

    Ebrahim Cheraghi

    2017-09-01

    Full Text Available Background Reproductive toxicity is a major challenge associated with aluminum (Al exposure. No studies have evaluated the possible effects of curcumin (CUR on Al-induced reproductive dysfunction. Therefore, this study investigated the effects of CUR treatment on Al-induced reproductive damage. Materials and Methods In this experimental study, 40 male Wistar rats were allocated to the five groups (n=8 based on the treatment they received: no treatment (control, solvent [dimethyl sulfoxide (DMSO or distilled water], CUR 10 mg/kg body weight (BW, Al chloride 10 mg/kg BW, and CUR+Al chloride (10 mg/kg BW/each alone. Treatments were performed by intraperitoneal (IP injections for 28 days. The left testis was assessed for histopathological analysis as well as the incidence of germ cell apoptosis. One-way analysis of variance (ANOVA followed by the Tukey’s test was used. P<0.05 was considered significant. A value of P<0.05 was considered significant. Results Significant reductions in body and testis weight; plasma testosterone and luteinizing hormone levels; sperm count, motility, morphology, and viability; germinal epithelium thickness; seminiferous tubules diameter; as well as, superoxide dismutase activity were observed in rats treated with Al. Moreover, Al exposure caused significant increments in the lumen diameter of tubules, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL-positive cells and malondialdehyde (MDA levels compared to the control group. However, in rats receiving CUR+Al, CUR significantly reversed the adverse effects of Al on testis and sperm quality. No significant differences in follicle-stimulating hormone (FSH levels and nuclear diameter of spermatogonia were detected among all groups. Conclusion It can be concluded that Al causes reproductive dysfunction by creating oxidative damage. CUR, on the other hand, reduces the toxic effects of Al and improves the antioxidant status and sperm quality in male rats.

  7. Protective effects and mechanisms of curcumin on podophyllotoxin toxicity in vitro and in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Li, Juan; Dai, Cai-Xia; Sun, Hua [Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632 (China); Jin, Lu [Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632 (China); State Key Laboratory of New Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203 (China); Guo, Chong-Yi; Cao, Wei; Wu, Jie; Tian, Hai-Yan [Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632 (China); Luo, Cheng [State Key Laboratory of New Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203 (China); Ye, Wen-Cai [Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632 (China); Jiang, Ren-Wang, E-mail: trwjiang@jnu.edu.cn [Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632 (China)

    2012-12-01

    Podophyllotoxin (POD) is a naturally occurring lignan with pronounced antineoplastic and antiviral properties. POD binds to tubulin and prevents the formation of mitotic spindle. Although cases of overdose or accidental ingestion are quite often, no specific therapy is currently available to treat the POD intoxication. In the current investigation, the protective effects and mechanisms of curcumin (CUR) on podophyllotoxin toxicity were evaluated in vitro and in vivo. The results showed that CUR could protect POD-induced cytotoxicity by recovering the G2/M arrest and decrease the changes of membrane potential and microtubule structure in Vero cells. A significant decrease of mortality rates was observed in Swiss mice treated by intragastrical administration of POD + CUR as compared with POD alone. The POD + CUR group also exhibited decreases in plasma transaminases, alkaline phosphatase, lactate dehydrogenase, plasma urea, creatinine and malondialdehyde level but elevated superoxide dismutase and glutathione levels as compared to the POD group. Histological examination of the liver and kidney demonstrated less morphological changes in the treatment of POD + CUR as compared with POD alone. The mechanism of the protective effects might be due to the competitive binding of CUR with POD in the same colchicines binding site as revealed by the tubulin polymerization assay and the molecular docking analysis, and the antioxidant activity against the oxidative stress induced by POD. In summary, both in vitro and in vivo data indicated the promising role of CUR as a protective agent against the POD poisoning. Highlights: ► A potential antidote to treat the podophyllotoxin (POD) intoxication is found. ► Curcumin showed promising effects against POD poisoning in vitro and in vivo. ► The mechanisms lie in the antioxidant activity and competitive binding with tubulin.

  8. Curcumin attenuates morphine antinociceptive tolerance through suppressing up-regulation of spinal Toll-like receptor 4 in rats

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    Fei GAO

    2017-12-01

    Full Text Available Objective To investigate the effects of curcumin (Cur on activation of spinal Toll-like receptor 4 (TLR4 and on the chronic antinociceptive tolerance of morphine. Methods Sixty male Sprague-Dawley rats with successful intrathecal catheterization were randomly divided into four groups (n=15: saline (NS group; morphine (MOR group; curcumin (Cur group and morphine plus curcumin (MOR+Cur group. A morphine tolerance model of rats was induced by intrathecal injection of morphine 15μg, once a day for 7 consecutive days in MOR and MOR+Cur group; 100μg curcumin was administered intrathecally once a day for 7 consecutive days in Cur and MOR+Cur group, 10μl saline was administered intrathecally once a day for 7 consecutive days in NS group. The effect of curcumin intrathecal catheterization on morphine antinociceptive tolerance was explored by the tail flick latency (TFL method and mechanical withdrawal threshold (MWT, and then the maximum possible potential effect (MPE was calculated. The immunofluorescence staining method was applied to detect the effect of curcumin on the activation of lumbar spinal microglia. Real-time PCR and Western blotting were used to evaluate the effect of curcumin on the expression of mRNA and protein of spinal TLR4. Results The %MPE TFL and %MPE MWT increased significantly in MOR+Cur group than in MOR group (P0.05. The lumbar spinal microglia increased markedly and the expressions of polyclonal antibody IBA-1 and TLR4 were significantly up-regulated in MOR group than in NS group (P0.05. Conclusion Curcumin may attenuate chronic morphine antinociceptive tolerance through inhibiting spinal TLR4 up-regulation. DOI: 10.11855/j.issn.0577-7402.2017.12.06

  9. Increased chemopreventive effect by combining arctigenin, green tea polyphenol and curcumin in prostate and breast cancer cells

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    Wang, Piwen; Wang, Bin; Chung, Seyung; Wu, Yanyuan; Henning, Susanne M.; Vadgama, Jaydutt V.

    2014-01-01

    The low bioavailability of most flavonoids limits their application as anti-carcinogenic agents in humans. A novel approach of treatment with a mixture of bioactive compounds that share molecular anti-carcinogenic targets may enhance the effect on these targets at low concentrations of individual compound, thereby overcoming the limitations of reduced bioavailability. We therefore investigated whether a combination of three natural products arctigenin (Arc), a novel anti-inflammatory lignan from the seeds of Arctium lappa, green tea polyphenol (−)-epigallocatechin gallate (EGCG) and curcumin (Cur) increases the chemopreventive potency of individual compounds. LNCaP prostate cancer and MCF-7 breast cancer cells were treated with 2–4 mg/L (about 5–10μM) Cur, 1μM Arc and 40μM EGCG alone or in combination for 48h. In both cell lines treatment with the mixture of Cur, Arc and EGCG synergistically increased the antiproliferative effect. In LNCaP cells both Arc and EGCG increased the pro-apoptotic effect of Cur. Whereas in MCF-7 cells Arc increased the cell apoptosis of Cur while EGCG enhanced cell cycle arrest of Cur at G0/G1 phase. The strongest effects on cell cycle arrest and apoptosis were achieved by combining all three compounds in both cell lines. The combination treatment significantly increased the ratio of Bax to Bcl-2 proteins, decreased the activation of NFκB, PI3K/Akt and Stat3 pathways and cell migration compared to individual treatment. These results warrant in vivo studies to confirm the efficacy of this novel regimen by combining Arc and EGCG with Cur to enhance chemoprevention in both prostate and breast cancer. PMID:25243063

  10. Sequel of MgO nanoparticles in PLACL nanofibers for anti-cancer therapy in synergy with curcumin/β-cyclodextrin

    International Nuclear Information System (INIS)

    Sudakaran, Shruthi Vathaluru; Venugopal, Jayarama Reddy; Vijayakumar, Gnaneshwar Puvala; Abisegapriyan, Sivasubramanian; Grace, Andrews Nirmala; Ramakrishna, Seeram

    2017-01-01

    Pharmaceutical industries spend more money in developing new and efficient methods for delivering successful drugs for anticancer therapy. Electrospun nanofibers and nanoparticles loaded with drugs have versatile biomedical applications ranging from wound healing to anticancer therapy. We aimed to attempt for fabricating elastomeric poly (L-lactic acid-co-ε-caprolactone) (PLACL) with Aloe Vera (AV), magnesium oxide (MgO) nanoparticles, curcumin (CUR) and β-cyclodextrin (β-CD) composite nanofibers to control the growth of MCF-7 cells for breast cancer therapy. The study focused on the interaction of MgO nanoparticle with CUR and β-CD inhibiting the proliferation of Michigan Cancer Foundation-7 (MCF-7) breast cancer cells. FESEM micrographs of fabricated electrospun PLACL, PLACL/AV, PLACL/AV/MgO, PLACL/AV/MgO/CUR and PLACL/AV/MgO/β-CD nanofibrous scaffolds achieved bead free, random and uniform nanofibers with fiber diameter in the range of 786 ± 286, 507 ± 171, 334 ± 95, 360 ± 94 and 326 ± 80 nm respectively. Proliferation of MCF-7 cells was decreased by 65.92% in PLACL/AV/MgO/CUR with respect to PLACL/AV/MgO nanofibrous scaffolds on day 9. The obtained results proved that 1% CUR interacting with MgO nanoparticles showed higher inhibition of MCF-7 cells among all other nanofibrous scaffolds thus serving as a promising biocomposite material system for the breast cancer therapy. - Highlights: • Biocomposite nanofibrous scaffolds fabricated bead free, uniform and possess rough fiber surface • Aloe Vera and MgO nanoparticles initiates cell adhesion and proliferation • CUR (1%)/MgO nanoparticle showed higher level of cell inhibition of MCF-7 cells compared to all other nanofibrous scaffolds • PLACL/AV/MgO/CUR nanofibrous scaffolds proved to be a potential material system for cancer therapy

  11. Suppressing the cytotoxicity of CuO nanoparticles by uptake of curcumin/BSA particles.

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    Zhang, Wenjing; Jiang, Pengfei; Chen, Ying; Luo, Peihua; Li, Guanqun; Zheng, Botuo; Chen, Wei; Mao, Zhengwei; Gao, Changyou

    2016-05-05

    The adverse effects of metal-based nanoparticles on human beings and the environment have received extensive attention recently. It is urgently required to develop a simple and effective method to suppress the toxicity of metal-based nanomaterials. In this study, a hydrophobic antioxidant and a chelation agent curcumin (CUR) were encapsulated into bovine serum albumin (BSA) particles by a simple co-precipitation method, and followed by glutaraldehyde cross-linking. The CUR/BSA particles had an average size of 300 nm in diameter with a negatively charged surface and sustained curcumin release properties. The cellular uptake and cytotoxicity of CUR/BSA particles were followed on A549 cells, HepG2 cells and RAW264.7 cells. The CUR/BSA particles had higher intracellular accumulation and lower cytotoxicity compared with the free curcumin at the same drug concentration. The CUR/BSA particles could suppress the cytotoxicity generated by CuO nanoparticles as a result of decrease of both the intracellular reactive oxygen species (ROS) level and Cu(2+) concentration, while the free curcumin did not show any obvious detoxicating effect. The detoxicating effects of CUR/BSA particles were further studied in an intratracheal instillation model in vivo, demonstrating significant reduction of toxicity and inflammatory response in rat lungs induced by CuO nanoparticles. The concept-proving study demonstrates the potential of the CUR/BSA particles in suppressing cytotoxicity of metal-based nanomaterials, which is a paramount requirement for the safe application of nanotechnology.

  12. Protective Effect of Curcumin Against Oxidative Stress-Induced Injury in Rats with Parkinson’s Disease Through the Wnt/ β-Catenin Signaling Pathway

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    Yun-Liang Wang

    2017-10-01

    Full Text Available Background/Aims: The study aimed to investigate the protective effect of curcumin against oxidative stress-induced injury of Parkinson’s disease (PD through the Wnt/β-catenin signaling pathway in rats. Methods: The successfully established PD rat models and normal healthy rats were randomly assigned into the 6-hydroxydopamine (6-OHDA, the curcumin (Cur and the control groups. Immunohistochemistry was used to detect the positive expression of tyrosine hydroxylase (TH, dopamine transporter (DAT and glial fibrillary acidic protein (GFAP. Deutocerebrum primary cells were extracted and classified into the control, 6-OHDA, Cur (5, 10, 15 µmol/L, Dickkopf-1 (DKK-1 and Cur + DKK-1 groups. MTT assays, adhesion tests and TUNEL staining were used to assess cell viability, adhesion and apoptosis, respectively. Western blotting and qRT-PCR were used to examine the protein and mRNA expressions of Wnt3a and β-catenin and the c-myc and cyclinD1 mRNA expressions. Results: TH and DAT expressions in the Cur group were elevated and GFAP was reduced compared with the 6-OHDA group. Curcumin enhanced viability, survival and adhesion and attenuated apoptosis of deutocerebrum primary cells by activating the Wnt/β-catenin signaling pathway. Higher Wnt3a and β-catenin mRNA and protein expressions and c-myc and cyclinD1 mRNA expressions, enhanced superoxide dismutase (SOD and glutathione peroxidase (GSH-Px contents, decreased malondialdehyde (MDA content and elevated mitochondrial membrane potential (∆ψm were found in the 10 and 15 µmol/L Cur groups compared with the 6-OHDA group. However, opposite tendencies were found in the Cur + DKK-1 group compared to the 10 µmol/L Cur group. Conclusion: This study suggests that curcumin could protect against oxidative stress-induced injury in PD rats via the Wnt/β-catenin signaling pathway.

  13. Antitumor and Antiangiogenic Activities of Curcumin in Cervical Cancer Xenografts in Nude Mice

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    Pornphrom Yoysungnoen-Chintana

    2014-01-01

    Full Text Available To evaluate the effects of curcumin (CUR on tumor progression and angiogenesis in cervical cancer- (CaSki- implanted nude mice and on the angiogenic biomarkers: vascular endothelial growth factor (VEGF, cyclooxygenase-2 (COX-2, and epidermal growth factor receptor (EGFR. CaSki cells were subcutaneously injected in nude mice to establish subcutaneous tumors. One month after injection, mice were orally administered vehicle or 500, 1,000, and 1,500 mg/kg of CUR daily × 30 consecutive days. Tumor volume was measured every 3-4 days. At the end of the study, tumor microvasculature was observed under confocal microscope, and immunohistochemical analyses were performed to detect CD31, VEGF, COX-2, and EGFR. CUR at the doses of 1,000 and 1,500 mg/kg showed significant tumor growth retardation (21.03% and 35.57% versus CaSki + vehicle group. The microvascular density (MVD in CaSki + vehicle group was significantly increased versus Control + vehicle group and significantly reduced by CUR (1,000 and 1,500 mg/kg. VEGF, COX-2, and EGFR expressions were upregulated in CaSki + vehicle group and attenuated significantly by CUR (1,000 and 1,500 mg/kg. In conclusion, high dose CUR inhibited tumor growth and angiogenesis in CaSki-implanted mice probably mediated by the downregulation of VEGF, COX-2 and EGFR. CUR may have a role in treating human cervical cancer and should be explored further.

  14. Suppressing the cytotoxicity of CuO nanoparticles by uptake of curcumin/BSA particles

    Science.gov (United States)

    Zhang, Wenjing; Jiang, Pengfei; Chen, Ying; Luo, Peihua; Li, Guanqun; Zheng, Botuo; Chen, Wei; Mao, Zhengwei; Gao, Changyou

    2016-05-01

    The adverse effects of metal-based nanoparticles on human beings and the environment have received extensive attention recently. It is urgently required to develop a simple and effective method to suppress the toxicity of metal-based nanomaterials. In this study, a hydrophobic antioxidant and a chelation agent curcumin (CUR) were encapsulated into bovine serum albumin (BSA) particles by a simple co-precipitation method, and followed by glutaraldehyde cross-linking. The CUR/BSA particles had an average size of 300 nm in diameter with a negatively charged surface and sustained curcumin release properties. The cellular uptake and cytotoxicity of CUR/BSA particles were followed on A549 cells, HepG2 cells and RAW264.7 cells. The CUR/BSA particles had higher intracellular accumulation and lower cytotoxicity compared with the free curcumin at the same drug concentration. The CUR/BSA particles could suppress the cytotoxicity generated by CuO nanoparticles as a result of decrease of both the intracellular reactive oxygen species (ROS) level and Cu2+ concentration, while the free curcumin did not show any obvious detoxicating effect. The detoxicating effects of CUR/BSA particles were further studied in an intratracheal instillation model in vivo, demonstrating significant reduction of toxicity and inflammatory response in rat lungs induced by CuO nanoparticles. The concept-proving study demonstrates the potential of the CUR/BSA particles in suppressing cytotoxicity of metal-based nanomaterials, which is a paramount requirement for the safe application of nanotechnology.

  15. The association between a low cerebro-umbilical ratio at 30-34 weeks gestation, increased intrapartum operative intervention and adverse perinatal outcomes.

    Science.gov (United States)

    Twomey, Sarah; Flatley, Christopher; Kumar, Sailesh

    2016-08-01

    The aim of this study was to investigate the relationship between the cerebro-umbilical ratio (CUR), measured at 30-34 weeks, and adverse intrapartum and perinatal outcomes. This was a retrospective cross-sectional cohort study of women delivering at the Mater Mothers' Hospital in Brisbane, Australia. Fetal Doppler indices for 1224 singleton pregnancies were correlated with maternal demographics and intrapartum and perinatal outcomes. Only women who attempted vaginal delivery were included in the study. Infants delivered by emergency cesarean section for fetal compromise had the lowest median CUR, 1.65 (IQR 1.17-2.12), compared to any other delivery group. The proportion of infants with a CUR ≤1 who required emergency cesarean section for fetal compromise was 33.3% compared to 9.3% of infants with a CUR >1 (adjusted OR 6.92 (95% CI 2.04-25.75), p<0.001). However, the detection rate of CUR ≤1 as a predictor for emergency cesarean delivery for fetal compromise was poor (18.9%). Detection rates increased in cohorts of infants born within two weeks of the scan or with birth weights <10th centile or <5th centile. Additionally, a CUR ≤1 was associated with lower median birth weight, higher rates of admission to the neonatal critical care unit and increased neonatal mortality. This study suggests that a CUR ≤1, measured at 30-34 weeks, is associated with a greater risk of emergency cesarean delivery for fetal compromise and a number of other adverse perinatal outcomes. The association was strongest in low birth weight babies. Copyright © 2016. Published by Elsevier Ireland Ltd.

  16. Curcumin Attenuates Hepatotoxicity Induced by Zinc Oxide Nanoparticles in Rats

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    Layasadat Khorsandi

    2016-06-01

    Full Text Available Background: Zinc oxide nanoparticles (NZnO are increasingly used in modern life. Most metal nanoparticles have adverse effects on the liver. Aims: To explore the protective action of curcumin (Cur against hepatotoxicity induced by NZnO in rats. Study Design: Animal experimentation. Methods: Control group animals received normal saline, while the Cur group animals were treated with 200 mg/kg of Cur orally for 21 days. NZnO-intoxicated rats received 50 mg/kg of NZnO for 14 days by gavage method. In the NZnO+Cur group, rats were pretreated with Cur for 7 days before NZnO administration. Plasma activities of Alanine aminotransferase (ALT, aspartate aminotransferase (AST and alkaline phosphatase (ALP were measured as biomarkers of hepatotoxicity. Hepatic levels of malondialdehyde (MDA and superoxide dismutase (SOD and glutathione peroxidase (GPx activities were measured for detection of oxidative stress in liver tissue. Histological changes and apoptosis in liver tissue were studied by using Hematoxylin-eosin staining and the transferase dUTP nick end labeling (TUNEL method. Results: NZnO induced a significant increase in plasma AST (2.8-fold, ALT (2.7-fold and ALP (1.97-fold activity in comparison to the control group (p<0.01. NZnO increased MDA content and reduced SOD and GPx activities. NZnO caused liver damage including centrilobular necrosis and microvesicular steatosis. The percentage of apoptosis in hepatocytes was increased in NZnO-treated rats (p<0.01. Pre-treatment of Cur significantly reduced lipid peroxidation (39%, increased SOD (156% and GPx (26% activities, and attenuated ALT (47%, AST (41% and ALP (30% activities. Pre-treatment with Cur also decreased the histology changes and apoptotic index of hepatocytes (p<0.05. Conclusion: These findings indicate that Cur effectively protects against NZnO-induced hepatotoxicity in rats. However, future studies are required to propose Cur as a potential protective agent against hepatotoxicity

  17. Folic acid-targeted disulfide-based cross-linking micelle for enhanced drug encapsulation stability and site-specific drug delivery against tumors

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    Zhang Y

    2016-03-01

    Full Text Available Yumin Zhang,1,* Junhui Zhou,2,* Cuihong Yang,1 Weiwei Wang,3 Liping Chu,1 Fan Huang,1 Qiang Liu,1 Liandong Deng,2 Deling Kong,3 Jianfeng Liu,1 Jinjian Liu1 1Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, 2Department of Polymer Science and Technology, School of Chemical Engineering and Technology, Tianjin University, 3Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, People’s Republic of China *These authors contributed equally in this work Abstract: Although the shortcomings of small molecular antitumor drugs were efficiently improved by being entrapped into nanosized vehicles, premature drug release and insufficient tumor targeting demand innovative approaches that boost the stability and tumor responsiveness of drug-loaded nanocarriers. Here, we show the use of the core cross-linking method to generate a micelle with enhanced drug encapsulation ability and sensitivity of drug release in tumor. This kind of micelle could increase curcumin (Cur delivery to HeLa cells in vitro and improve tumor accumulation in vivo. We designed and synthesized the core cross-linked micelle (CCM with polyethylene glycol and folic acid-polyethylene glycol as the hydrophilic units, pyridyldisulfide as the cross-linkable and hydrophobic unit, and disulfide bond as the cross-linker. CCM showed spherical shape with a diameter of 91.2 nm by the characterization of dynamic light scattering and transmission electron microscope. Attributed to the core cross-linking, drug-loaded CCM displayed higher Nile Red or Cur-encapsulated stability and better sensitivity to glutathione than noncross-linked micelle (NCM. Cellular uptake and in vitro antitumor studies proved the enhanced endocytosis and better cytotoxicity of CCM-Cur against

  18. Metabolomics reveals metabolic targets and biphasic responses in breast cancer cells treated by curcumin alone and in association with docetaxel.

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    Mathilde Bayet-Robert

    Full Text Available BACKGROUND: Curcumin (CUR has deserved extensive research due to its anti-inflammatory properties, of interest in human diseases including cancer. However, pleiotropic even paradoxical responses of tumor cells have been reported, and the mechanisms of action of CUR remain uncompletely elucidated. METHODOLOGY/PRINCIPAL FINDINGS: (1H-NMR spectroscopy-based metabolomics was applied to get novel insight into responses of MCF7 and MDA-MB-231 breast cancer cells to CUR alone, and MCF7 cells to CUR in cotreatment with docetaxel (DTX. In both cell types, a major target of CUR was glutathione metabolism. Total glutathione (GSx increased at low dose CUR (≤ 10 mg.l(-1-28 µM- (up to +121% in MCF7 cells, P<0.01, and +138% in MDA-MB-231 cells, P<0.01, but decreased at high dose (≥ 25 mg.l(-1 -70 µM- (-49%, in MCF7 cells, P<0.02, and -56% in MDA-MB-231 cells, P<0.025. At high dose, in both cell types, GSx-related metabolites decreased, including homocystein, creatine and taurine (-60 to -80%, all, P<0.05. Together with glutathione-S-transferase actvity, data established that GSx biosynthesis was upregulated at low dose, and GSx consumption activated at high dose. Another major target, in both cell types, was lipid metabolism involving, at high doses, accumulation of polyunsaturated and total free fatty acids (between ×4.5 and ×11, P<0.025, and decrease of glycerophospho-ethanolamine and -choline (about -60%, P<0.025. Multivariate statistical analyses showed a metabolic transition, even a biphasic behavior of some metabolites including GSx, between low and high doses. In addition, CUR at 10 mg.l(-1 in cotreatment with DTX induced modifications in glutathione metabolism, lipid metabolism, and glucose utilization. Some of these changes were biphasic depending on the duration of exposure to CUR. CONCLUSIONS/SIGNIFICANCE: Metabolomics reveals major metabolic targets of CUR in breast cancer cells, and biphasic responses that challenge the widely accepted

  19. The Effects of Men's Hostility Toward Women, Acute Alcohol Intoxication, and Women's Condom Request Style on Men's Condom Use Resistance Tactics.

    Science.gov (United States)

    Wegner, Rhiana; Davis, Kelly Cue; Stappenbeck, Cynthia A; Kajumulo, Kelly F; Norris, Jeanette; George, William H

    2017-10-01

    To extend previous research demonstrating that intoxicated men high in hostility toward women report stronger intentions to use coercive condom use resistance (CUR) tactics to have unprotected sex by examining the role of women's condom request style. Community, non-problem drinking men, ages 21-30 ( N = 296) completed standard alcohol administration procedures and read an eroticized story of a casual sexual interaction. Following the woman's request to use a condom, intentions to engage in coercive CUR tactics were assessed. Generalized linear models with gamma distributions assessed the 3-way interaction of men's hostility toward women, beverage condition (alcohol or sober), and the woman's condom request style (indirect, direct, or insistent). The 3-way interaction between hostility toward women, beverage condition, and indirect condom request (vs. direct) significantly predicted men's coercive CUR intentions. Men high in hostility toward women reported stronger CUR intentions after experiencing an indirect condom request, when sober or intoxicated, and after a direct condom request when intoxicated. Men high in hostility toward women reported the weakest CUR intentions when sober following an insistent or direct condom request. Men high in hostility toward women pose a threat to women's sexual safety, particularly when intoxicated or following an indirect condom request. Hostility toward women and alcohol consumption should be addressed in sexual risk prevention programs. Pending further replication, women should be informed of the relative effectiveness of using insistent condom requests.

  20. Rescuing apoptotic neurons in Alzheimer's disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin.

    Science.gov (United States)

    Kuo, Yung-Chih; Lin, Ching-Chun

    2015-01-01

    Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood-brain barrier and treat Alzheimer's disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were used to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced by β-amyloid1-42 (Aβ(1-42)) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute zeta potential value, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ(1-42), but decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability of NGF and CUR across the blood-brain barrier, but reduced the absolute zeta potential value and biocompatibility. WGA-CL-liposomes carrying NGF and CUR could be promising colloidal delivery carriers for future clinical application in targeting the blood-brain barrier and inhibiting neurotoxicity.

  1. Acupuncture for Chronic Urinary Retention due to Spinal Cord Injury: A Systematic Review

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    Jia Wang

    2016-01-01

    Full Text Available No systematic review has been published on the use of acupuncture for the treatment of chronic urinary retention (CUR due to spinal cord injury (SCI. The aim of this review was to assess the effectiveness and safety of acupuncture for CUR due to SCI. Three randomized controlled trials (RCTs including 334 patients with CUR due to SCI were included. Meta-analysis showed that acupuncture plus rehabilitation training was much better than rehabilitation training alone in decreasing postvoid residual (PVR urine volume (MD −109.44, 95% CI −156.53 to −62.35. Likewise, a combination of acupuncture and aseptic intermittent catheterization was better than aseptic intermittent catheterization alone in improving response rates (RR 1.23, 95% CI 1.10 to 1.38. No severe adverse events were reported. In conclusion, acupuncture as a complementary therapy may have a potential effect in CUR due to SCI in decreasing PVR and improving bladder voiding. Additionally, acupuncture may be safe in treating CUR caused by SCI. However, due to the lack of high quality RCTs, we could not draw any definitive conclusions. More well-designed RCTs are needed to provide strong evidence.

  2. Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin delivery.

    Science.gov (United States)

    Liu, Jianfeng; Liu, Jinjian; Xu, Hongyan; Zhang, Yumin; Chu, Liping; Liu, Qingfen; Song, Naling; Yang, Cuihong

    2014-01-01

    The poor aqueous solubility and low bioavailability of curcumin restrict its clinical application for cancer treatment. In this study, a novel tumor-targeting nanofiber carrier was developed to improve the solubility and tumor-targeting ability of curcumin using a self-assembled Nap-GFFYG-RGD peptide. The morphologies of the peptide nanofiber and the curcumin-encapsulated nanofiber were visualized by transmission electron microscopy. The tumor-targeting activity of the curcumin-encapsulated Nap-GFFYG-RGD peptide nanofiber (f-RGD-Cur) was studied in vitro and in vivo, using Nap-GFFYG-RGE peptide nanofiber (f-RGE-Cur) as the control. Curcumin was encapsulated into the peptide nanofiber, which had a diameter of approximately 10-20 nm. Curcumin showed sustained-release behavior from the nanofibers in vitro. f-RGD-Cur showed much higher cellular uptake in αvβ3 integrin-positive HepG2 liver carcinoma cells than did non-targeted f-RGE-Cur, thereby leading to significantly higher cytotoxicity. Ex vivo studies further demonstrated that curcumin could accumulate markedly in mouse tumors after administration of f-RGD-Cur via the tail vein. These results indicate that Nap-GFFYG-RGD peptide self-assembled nanofibers are a promising hydrophobic drug delivery system for targeted treatment of cancer.

  3. Synthesis and characterization of curcumin loaded PLA-Hyperbranched polyglycerol electrospun blend for wound dressing applications.

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    Perumal, Govindaraj; Pappuru, Sreenath; Chakraborty, Debashis; Maya Nandkumar, A; Chand, Dillip Kumar; Doble, Mukesh

    2017-07-01

    This study is aimed to develop curcumin (Cur) incorporated electrospun nanofibers of a blend of poly (lactic acid) (PLA) and hyperbranched polyglycerol (HPG) for wound healing applications. Both the polymers are synthesized and fabricated by electrospinning technique. The produced nanofibers were characterized by Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Colorimetry (DSC) and Thermogravimetric Analysis (TGA). Electrospun scaffolds (PLA/HPG/Cur) exhibits very high hydrophilicity, high swelling and drug uptake and promotes better cell viability, adhesion and proliferation when compared to PLA/Cur electrospun nanofibers. Biodegradation study revealed that the morphology of the nanofibers were unaffected even after 14days immersion in Phosphate Buffered Saline. In vitro scratch assay indicates that migration of the cells in the scratch treated with PLA/HPG/Cur is complete within 36h. These results suggest that PLA/HPG/Cur nanofibers can be a potential wound patch dressing for acute and chronic wound applications. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Curcumin decreases astrocytic reaction after gliotoxic injury in the rat brainstem

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    Eduardo Bondan

    Full Text Available ABSTRACT Recent studies have demonstrated that curcumin (Cur has antioxidant, anti-inflammatory and anti-fibrotic effects. Ethidium bromide (EB injections into the central nervous system (CNS are known to induce local oligodendroglial and astrocytic loss, resulting in primary demyelination and neuroinflammation. Peripheral astrogliosis is seen around the injury site with increased immunoreactivity to glial fibrillary acidic protein (GFAP. This investigation aimed to evaluate the effect of Cur administration on astrocytic response following gliotoxic injury. Wistar rats were injected with EB into the cisterna pontis and treated, or not, with Cur (100 mg/kg/day, intraperitoneal route during the experimental period. Brainstem sections were collected at 15, 21 and 31 days after EB injection and processed for GFAP immunohistochemical staining. Astrocytic reactivity was measured in a computerized system for image analysis. In Cur-treated rats, the GFAP-stained area around the lesion was significantly smaller in all periods after EB injection compared to untreated animals, showing that Cur reduces glial scar development following injury.

  5. Development of antibacterial paper coated with sodium hyaluronate stabilized curcumin-Ag nanohybrid and chitosan via polyelectrolyte complexation for medical applications

    Science.gov (United States)

    Rao Kummara, Madhusudana; Kumar, Anuj; Soo, Han Sung

    2017-11-01

    Sodium hyaluronate (HA) stabilized curcumin-Ag (Cur-Ag) hybrid nanoparticles were prepared in the water-ethanol mixture under constant mechanical stirring condition. The obtained HA stabilized Cur-Ag hybrid nanoparticles were characterized by fourier transform infrared spectroscopy, UV-visible spectroscopy, and x-ray diffraction to confirm the formation and structural interactions. The obtained Cur-Ag hybrid nanoparticles showed spherical shape with their size range 5-12 nm that was increased with the increasing a amount of silver ions as confirmed by transmission electron microscopic analysis. Further, a fibrous cellulose filter paper was impregnated with these hybrid nanoparticles and chitosan (CS) as biopolymer via polyelectrolyte complexation. The morphological analysis confirmed the uniform distribution of hybrid nanoparticle system onto the cellulose fibers of the fibrous filter paper. As per disc diffusion method, the Cur-Ag hybrid nanoparticles impregnated CS-coated filter paper exhibited excellent antibacterial properties against gram-negative Escherichia coli (E.coli) bacteria compared to HA stabilized Cur only. Moreover, as prepared hybrid nanoparticles impregnated biocomposite system is eco-friendly with efficient antibacterial property and have good potential to be used in medical applications.

  6. Curcumin ameliorates diclofenac sodium-induced nephrotoxicity in male albino rats.

    Science.gov (United States)

    Ahmed, Ahmady Y; Gad, Amany M; El-Raouf, Ola M Abd

    2017-10-01

    Exposure to drugs often results in toxicity in the kidney which represents the major control system maintaining homeostasis of the body and thus is especially susceptible to xenobiotics. Nephrotoxicity is a life-threatening side-effect of nonsteroidal anti-inflammatory drugs (NSAIDs). Diclofenac is one of the most frequently prescribed NSAIDs and have been reported to cause multiple organs damage. Curcumin (CUR) exhibits nephroprotective properties. Therefore, rats were divided into four groups; rats of groups 3 and 4 received diclofenac (100 mg/kg, i.m.), whereas rats of groups 2 and 4 received CUR (100 mg/kg, p.o.) for 3 days. Diclofenac revealed a significant increase in urea and creatinine levels and malondialdehyde concentration and marked reduction in catalase activity and reduced glutathione concentration. Histopathologically, diclofenac produced fatty changes and eosinophilic casts were detected in the renal tubules, those were attenuated by administration of CUR prior diclofenac. © 2017 Wiley Periodicals, Inc.

  7. Effect of concentration of Curcuma longa L. on chitosan-starch based edible coating

    Science.gov (United States)

    Yusof, N. M.; Jai, J.; Hamzah, F.; Yahya, A.; Pinijsuwan, S.

    2017-08-01

    The ability of chitosan-starch based coating to extend shelf life of strawberry were studied. The main objectives of this paper is to study the effects of different concentrations (20, 15, 10 and 5 µL) of Curcuma longa L. (CUR) essential oil into chitosan-based edible coating on surface tension in order to increase the effectiveness of the coating. CUR or turmeric is one of the commercially planted herbs in Malaysia for its phytochemical benefits. Application of edible coating using dipping technique has been analysed and evaluated for their effectiveness in extending shelf life of fruits. Surface tension was analysed to investigate the adhesion properties. The best CUR concentration was 15 µL with the optimum surface tension was found to be 31.92 dynes/cm.

  8. Què ha de tenir en compte l'usuari d'ordinador?

    OpenAIRE

    Valls Oriol, Maria Mercè

    2014-01-01

    El treball que aquí es presenta és la creació d'un curs online pilot amb la plataforma online i gratuïta Chamilo per realitzar formació en l'empresa Consorci Sanitari (que no té plataforma virtual d'aprenentatge implantada). La temàtica del curs es basa en tot el que fa referència a l'ergonomia relacionada amb la utilització dels ordinadors. La creació del curs s'ha realitzat amb la metodologia ADDIE obtenint resultats positius, fet que conclou que la implementació d'una plataforma virtual d'...

  9. Curcumin-conjugated magnetic nanoparticles for detecting amyloid plaques in Alzheimer's disease mice using magnetic resonance imaging (MRI).

    Science.gov (United States)

    Cheng, Kwok Kin; Chan, Pui Shan; Fan, Shujuan; Kwan, Siu Ming; Yeung, King Lun; Wáng, Yì-Xiáng J; Chow, Albert Hee Lum; Wu, Ed X; Baum, Larry

    2015-03-01

    Diagnosis of Alzheimer's disease (AD) can be performed with the assistance of amyloid imaging. The current method relies on positron emission tomography (PET), which is expensive and exposes people to radiation, undesirable features for a population screening method. Magnetic resonance imaging (MRI) is cheaper and is not radioactive. Our approach uses magnetic nanoparticles (MNPs) made of superparamagnetic iron oxide (SPIO) conjugated with curcumin, a natural compound that specifically binds to amyloid plaques. Coating of curcumin-conjugated MNPs with polyethylene glycol-polylactic acid block copolymer and polyvinylpyrrolidone by antisolvent precipitation in a multi-inlet vortex mixer produces stable and biocompatible curcumin magnetic nanoparticles (Cur-MNPs) with mean diameter <100 nm. These nanoparticles were visualized by transmission electron microscopy and atomic force microscopy, and their structure and chemistry were further characterized by X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, time-of-flight secondary ion mass spectrometry, and Fourier transform infrared spectroscopy. Cur-MNPs exhibited no cytotoxicity in either Madin-Darby canine kidney (MDCK) or differentiated human neuroblastoma cells (SH-SY5Y). The Papp of Cur-MNPs was 1.03 × 10(-6) cm/s in an in vitro blood-brain barrier (BBB) model. Amyloid plaques could be visualized in ex vivo T2*-weighted magnetic resonance imaging (MRI) of Tg2576 mouse brains after injection of Cur-MNPs, and no plaques could be found in non-transgenic mice. Immunohistochemical examination of the mouse brains revealed that Cur-MNPs were co-localized with amyloid plaques. Thus, Cur-MNPs have the potential for non-invasive diagnosis of AD using MRI. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Crystalline ethylene oxide and propylene oxide triblock copolymer solid dispersion enhance solubility, stability and promoting time-controllable release of Curcumin.

    Science.gov (United States)

    Alves, Thais Francine Ribeiro; Lopes, Franciely; Rebelo, Marcia; Souza, Juliana; Pontes, Katiusca; Santos, Carolina; Severino, Patricia; Junior, Jose; Komatsu, Daniel; Chaud, Marco Vinicius

    2018-01-17

    The design and development of an effective medicine are however often faced with a number of challenges. One of them is the close relationship of drug's bioavailability with solubility, dissolution rate and permeability. The use of curcumin's (CUR) therapeutic potential is limited by its poor water solubility and low chemical stability. The purpose was to evaluate the effect of polymer and solid dispersion (SD) preparation techniques to enhance the aqueous solubility, dissolution rate and stability of the CUR. The recent patents on curcumin SD were reported as (i) curcumin with polyvinylpyrrolidone (WO2006022012 and CN20151414227-20150715), (ii) curcumin-zinc/polyvinylpyrrolidone (WO2009079902), (iii) curcumin-poloxamer 188 (CN2008171177 20080605), (iv) curcumin SD prepared by melting method (CN20161626746-20160801). SD and physical mixture of CUR with Poloxamer-407, Hydroxypropylmetylcellulose-K4M and Polyvinylpyrrolidone-K30 were prepared at the ratios of 1:2; 1:1 and 2:1. The samples were evaluated by solubility, stability, dissolution rate and characterized by SEM, PXRD, DSC and FTIR. The solubility, stability (pH 7.0) and dissolution rate were significantly greater for SD (CUR:P-407 1:2). The PXRD,SEM and DSC indicated a change in the crystalline state of CUR. The enhancement of solubility was dependent on a combination of factors including the weight ratio, preparation techniques, and carrier properties. The drug release data fitted well with the Weibull equation, indicating that the drug release was controlled by diffusion, polymer relaxation and erosion occurring simultaneously. Thus, these SDs, specifically CUR:P-407 1:2 w/w, can overcome the barriers of poor bioavailability to reap its many beneficial properties. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  11. Curtovirus-cucurbit interaction: acquisition host plays a role in leafhopper transmission in a host-dependent manner.

    Science.gov (United States)

    Chen, Li-Fang; Gilbertson, Robert L

    2009-01-01

    Curly top disease (CTD) of vegetable crops is caused by viruses in the genus Curtovirus (family Geminiviridae). Cucurbits are reported to be susceptible to CTD; however, the disease is rare in California despite annual outbreaks in other hosts (e.g., common bean, pepper, sugar beet, and tomato). Consistent with these observations, no obvious curly top symptoms were observed in melon fields surveyed for CTD in Central California in 2004 and 2005, whereas the disease was readily observed in tomato plants in nearby fields. However, samples of cucurbits from Idaho with curly top-like symptoms, collected in 2005 and 2007, were confirmed to have the disease. The susceptibility of cucurbits (cantaloupe, honeydew melon, pumpkin, and watermelon) to the three curtoviruses known to occur in California (Beet curly top virus, BCTV; Beet severe curly top virus, BSCTV; and Beet mild curly top virus, BMCTV) was evaluated by agroinoculation or leafhopper transmission. Irrespective of the curtovirus species and inoculation method, low rates of infection and mild or symptomless disease phenotypes were observed in cucurbits. In contrast, all inoculated tomato, Nicotiana benthamiana, or shepherd's purse plants were infected and developed severe symptoms. In leafhopper transmission experiments, BMCTV infected cucurbits when leafhoppers acquired the virus from a symptomatic host with a high viral titer (shepherd's purse), whereas no infection occurred when the acquisition host had mild symptoms and a low viral titer (sugar beet); in contrast, the acquisition host did not influence transmission of BMCTV to tomato or shepherd's purse (all plants were infected). This revealed an influence of the acquisition host on leafhopper transmission in a host-specific manner. Our results also indicate that, although cucurbits can develop CTD, they are relatively poor hosts for these curtoviruses.

  12. Self-Monitoring and Self-Delivery of Photosensitizer-Doped Nanoparticles for Highly Effective Combination Cancer Therapy in Vitro and in Vivo.

    Science.gov (United States)

    Zhang, Jinfeng; Liang, Yu-Chuan; Lin, Xudong; Zhu, Xiaoyue; Yan, Li; Li, Shengliang; Yang, Xia; Zhu, Guangyu; Rogach, Andrey L; Yu, Peter K N; Shi, Peng; Tu, Lung-Chen; Chang, Chia-Ching; Zhang, Xiaohong; Chen, Xianfeng; Zhang, Wenjun; Lee, Chun-Sing

    2015-10-27

    Theranostic nanomedicine is capable of diagnosis, therapy, and monitoring the delivery and distribution of drug molecules and has received growing interest. Herein, a self-monitored and self-delivered photosensitizer-doped FRET nanoparticle (NP) drug delivery system (DDS) is designed for this purpose. During preparation, a donor/acceptor pair of perylene and 5,10,15,20-tetro (4-pyridyl) porphyrin (H2TPyP) is co-doped into a chemotherapeutic anticancer drug curcumin (Cur) matrix. In the system, Cur works as a chemotherapeutic agent. In the meantime, the green fluorescence of Cur molecules is quenched (OFF) in the form of NPs and can be subsequently recovered (ON) upon release in tumor cells, which enables additional imaging and real-time self-monitoring capabilities. H2TPyP is employed as a photodynamic therapeutic drug, but it also emits efficient NIR fluorescence for diagnosis via FRET from perylene. By exploiting the emission characteristics of these two emitters, the combinatorial drugs provide a real-time dual-fluorescent imaging/tracking system in vitro and in vivo, and this has not been reported before in self-delivered DDS which simultaneously shows a high drug loading capacity (77.6%Cur). Overall, our carrier-free DDS is able to achieve chemotherapy (Cur), photodynamic therapy (H2TPyP), and real-time self-monitoring of the release and distribution of the nanomedicine (Cur and H2TPyP). More importantly, the as-prepared NPs show high cancer therapeutic efficiency both in vitro and in vivo. We expect that the present real-time self-monitored and self-delivered DDS with multiple-therapeutic and multiple-fluorescent ability will have broad applications in future cancer therapy.

  13. Analisis Produksi USAha Pengasapan Ikan Di Desa Koto Mesjid Kecamatan XIII Koto Kampar Kabupaten Kampar

    OpenAIRE

    Sari, Yesi Purmita; ', Harlen; Harahap, Azwar

    2017-01-01

    The purpose of this research is to know the capital, raw materials and labor effect of positive and negative factors of production and to know what is the most dominant affect of curring fish industrial production at Koto Mesjid Kampar XIII District of Kampar.The population in this research is the whole industry entrepeneur fogging producing smoked fish/ fish salai in the village of Koto Mesjid that addup to 10 industry. Sampling techniques in the study using census by taking the whole of cur...

  14. Mange udfører dagligt et meget vigtig velfærdsarbejde

    DEFF Research Database (Denmark)

    Damsgaard, Janne Brammer

    2011-01-01

    Det er på tide, at vi, der har nydt og nyder godt af velfærdsstaten og dens engagerede medarbejdere og borgere, fortæller de gode historier om den vigtige menneskelige livsverdens-dimension. Det mener sygeplejerske og cand.cur. Janne Brammer Damsgaard......Det er på tide, at vi, der har nydt og nyder godt af velfærdsstaten og dens engagerede medarbejdere og borgere, fortæller de gode historier om den vigtige menneskelige livsverdens-dimension. Det mener sygeplejerske og cand.cur. Janne Brammer Damsgaard...

  15. Dėl kuršių kalbos palikimo

    Directory of Open Access Journals (Sweden)

    Vytautas Mažiulis

    2011-12-01

    Full Text Available ON THE HERITAGE OF CURONIANSummaryLithuanian dial. aukà/ aũkas “Opfer” (“victim, sacrifice” is from Lith. alkà / al̃̃kas “Hain”, which is from Balt. *alkā / *alkas „Hain“, which along with Goth. alks „Hain“ suggests that I.-E. *alk- „Hain“, is most likely derived from the I.-E. verb *alk- (: *alek- „to preserve, protect“. Perhaps the I.-E. verb *ol- / *el- „to flow“,“to twist, meander“ is hidden in it.The Samogitian hill Girgždū̃tė is in the place of the older Girgždutà < Cur. *Girzdutā, which is mobile subst. (fem. *girzdutā „that (stream, which gurgles, babbles“← verb. Cur. *girzd- „to gurgle“, a derivative from subst. Cur. *girzda- „gurgle, gravel“. Criticism is provided against the traditional opinion, that the name of the hill Cur. *Girzdutā is attested inaccurately to in historical sources (XIV—XV centuries.The settlement Lith. (Žem. Krãžiai, as the plur. collectivum, presupposes the former Cur. river/settlement *Kraźā́ from onomatopoetic Cur. (mobile subst. *kraźā́ „the murmurer“← verb. Cur. *kreź-/*kraź- „to murmur“. Afterwards, when the river *Kraźantē appeared, the form *Kraźā́ from river/settlement turned into the settlement name *Kraźā́. The issue of the West Balt. (and Cur. words for „ant“ are touched upon. First of all Prus. (E 791 sangis „ant“, is most likely from *zangis „speedy one“; further on the Lith. skruzdìs „ant“ is discussed. Also discussed is the Curonian origin of the Žem. place names Šatrijà and Var̃niai, and evidence is given, that the latter appeared not from the word „varna (crow“ which is the traditionally held view, but from „that, which is blackish“.

  16. [Dynamic accumulation regulation of curcumin, demethoxycurcumin and bisdemethoxyeurcumin in three strains of curcuma longae rhizome].

    Science.gov (United States)

    Li, Qing-Miao; Yang, Wen-Yu; Tang, Xue-Mei; Zhang, Mei; Zhou, Xian-Jian; Shu, Guang-Ming; Zhao, Jun-Ning; Fang, Qing-Mao

    2014-06-01

    The paper is aimed to study the dynamic accumulation regulation of curcumin (Cur), demethoxycurcumin (DMC) and bisdemethoxyeurcumin (BDMC) in three strains of Curcuma longa, and provide scientific references for formalized cultivation, timely harvesting, quality control and breeding cultivation of C. longa. The accumulation regulation of the three curcumin derivatives was basically the same in rhizome of three strains. The relative contents decreased along with plant development growing, while the accumulation per hectare increased with plant development growing. The accumulation of curcuminoids per hectare could be taken as the assessment standard for the best harvest time of C. longa. A3 was the best strain in terms of Cur and BDMC content.

  17. Shaping the manufacturing industry performance: MIDAS approach

    International Nuclear Information System (INIS)

    Turhan, Ibrahim M.; Sensoy, Ahmet; Hacihasanoglu, Erk

    2015-01-01

    We aim to find out whether the exchange rate (against US dollar) or the interest rate (in local currency) is a better variable in predicting the capacity utilization rate of manufacturing industry (CUR) of Turkey after the 2008 global financial crisis. In that manner, we implement dynamic mixed data sampling (MIDAS) regression model to forecast monthly changes in CUR by using daily changes in the exchange rate and the interest rate separately. The results show that exchange rate has a better forecast performance suggesting that it is a stronger determinant in shaping the manufacturing industry

  18. Demodex and rosacea: Is there a relationship?

    Directory of Open Access Journals (Sweden)

    Diana Gonzalez-Hinojosa

    2018-01-01

    Full Text Available Purpose: The objective of the study is to compare the frequency of Demodex on the eyelash follicle of patients with rosacea and referents without rosacea or ophthalmological disorders. Methods: This is a comparative, open, observational, and cross-sectional study that included 41 patients diagnosed with rosacea and 41 referents without rosacea diagnosis or ophthalmic alterations. The individuals underwent a slit-lamp examination in which two eyelashes per eyelid were removed with fine forceps. The presence of Demodex was sought by direct visualization under a light microscope. The results were expressed as “positive” when at least one mite on one lash was found and “negative” when no mite was identified. Chi-square test was used to compare the presence of mites in both groups. Results: Eighty-two study individuals (45 females and 37 males were included, of which 41 patients were diagnosed with rosacea and 41 were without rosacea or ophthalmic alterations. The average mean age was 37 years with a minimum of 19 and a maximum of 87 years. Of the 41 patients with rosacea, 31 had erythematotelangiectatic rosacea and 10 had papulopustular rosacea. There were no patients with phymatous or ocular rosacea. The presence of Demodex was found in 32 patients: 24 patients with rosacea diagnosis (16 of the erythematotelangiectatic subtype and 8 of papulopustular subtype and 8 patients without rosacea or ophthalmic alterations (P ≤ 0.001. Conclusion: Rosacea was found to be a statistically significant risk factor for Demodex infestation in eyelashes, irrespective of age and sex, with a higher prevalence in papulopustular variety.

  19. Ichthyosis Follicularis with Atrichia and Photophobia Syndrome: First Case Report in Thailand

    Directory of Open Access Journals (Sweden)

    Rattanavalai Nitiyarom

    2017-05-01

    Full Text Available We herein describe the first reported case of Ichthyosis Follicularis with Atrichia and Photophobia (IFAP syndrome in Thailand. A 6-year-old boy presented with a history of photophobia since 1 month of age. Then he developed widespread follicular hyperkeratotic papules and subtotal non-scarring alopecia by the age of 10 months and 5 years, respectively. Sparse eyelashes and nail dystrophy were also noted. No neurological abnormalities, systemic involvement, and hearing impairment were observed. The clinical manifestations were consistent with IFAP syndrome, although genetic testing did not confirm the diagnosis of this rare disorder.

  20. Use of body hair and beard hair in hair restoration.

    Science.gov (United States)

    Umar, Sanusi

    2013-08-01

    For many hair restoration patients with limited scalp donor hair it is possible to use nonhead hair sources to increase the potential follicle supply. Follicular unit extraction provides the hair restoration surgeon with a useful surgical means for accessing this valuable source of donor reserve. Nonhead hair can also be used to restore eyebrows, eyelashes, and moustaches. This article focuses on the use of body hair and beard in hair restoration. Discussed are the indications and effective techniques for performing hair transplants using non head hair donor sources, along with the pitfalls and risks of this surgical modality. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. Iris Recognition for Human Identification

    Science.gov (United States)

    Alandkar, Lajari; Gengaje, Sachin

    2010-11-01

    Iris recognition system is the biometric identification system. Iris has an intricate structure, uniqueness, stability, and natural protection. Due to these features of the iris it can be used for biometric identification. This system gives better performance than other biometric identification systems. A novel eyelash removal method for preprocessing of human iris images in a human iris recognition system is presented.. Discrete cosine transform (DCT) method is used for feature extraction. For matching of two-iris code Hamming distance calculation is used. EER value must be less for the optimum performance of the system.

  2. [The application of highly hydrophilic contact lenses in the Fuchs-Steven-Johnson syndrome (author's transl)].

    Science.gov (United States)

    Schulz, E

    1979-01-01

    In 3 patients with status after Fuchs-Steven-Johnson syndrome highly hydrophilic contact lenses (72% H2O-up take) were adapted and worn permanently. 2 patients had worn already for a long time usual HEMA-lenses. The lenses showed themselves to be favorable against mechanical irritation from cilia and accessory eyelashes and from scarring conjunctival conditions, and they affected favorably the signs of dessication of the cornea. Corneal defects healed, and the patients had subjectively no complaints. It is sometimes necessary to replace the tears. An increase in the corneal vascularisation already present could not be observed during the 18 months observation time.

  3. Mucocutaneous complications of intraarterial 5-bromodeoxyuridine and radiation

    Energy Technology Data Exchange (ETDEWEB)

    McCuaig, C.C.; Ellis, C.N.; Greenberg, H.S.; Hegarty, T.J.; Page, M.A. (Univ. of Michigan Medical Center, Ann Arbor (USA))

    1989-12-01

    5-Bromodeoxyuridine (BUDR), a halopyrimidine thymidine analogue, is incorporated into the DNA of dividing cells and causes photoradiosensitization. Twenty-five patients with malignant astrocytomas were treated with continuous intracarotid BUDR radiosensitization and radiotherapy for 8 1/2 weeks. Unique dose-limiting mucocutaneous complications were encountered. Ipsilateral facial dermatitis with epilation of eyebrows and eyelashes, ocular irritation, and bilateral nail dystrophy developed in all patients. Less common reactions included oral ulceration in six patients, body exanthem on the trunk in five, and atypical erythema multiforme major in one.

  4. Premature birth, respiratory distress, intracerebral hemorrhage, and silvery-gray hair: differential diagnosis of the 3 types of Griscelli syndrome.

    Science.gov (United States)

    Al-Idrissi, Eman; ElGhazali, Geyhad; Alzahrani, Mofarah; Ménasché, Gaël; Pachlopnik Schmid, Jana; Basile, Geneviève de Saint

    2010-08-01

    A preterm neonate, born to consanguineous parents, presented with respiratory distress, intracerebral hemorrhage, and a silvery-gray sheen of the hair and eyelashes. Griscelli syndrome (GS) type 3 was diagnosed after the detection of a novel homozygous mutation of the melanophilin gene. Thus, only the hypopigmentation, but not the patient's other clinical features, were attributable to this form of GS. Differential diagnosis of the various forms of GS must be performed as early as possible as GS2 is associated with a life threatening but curable immune disorder.

  5. The starry room naked eye astronomy in the intimate universe

    CERN Document Server

    Schaaf, Fred

    2002-01-01

    This inspiring and enriching book, a collection of essays that evokes the beauty and wonder of the night sky, explains to beginning skywatchers how to find and where to look for specific celestial objects. Author Fred Schaaf, once described as a naturalist in the tradition of Henry David Thoreau and John Burroughs, reveals an intense love of his subject and a keen knowledge of the optimum ways of viewing astronomical phenomena with the naked eye. Schaaf not only describes such special sights as an eyelash-thin moon, a shooting star, streaking comets, and a lunar eclipse, but he also explains w

  6. Diffuse heterochromia of scalp hair.

    Science.gov (United States)

    Lee, W S; Lee, I W; Ahn, S K

    1996-11-01

    Heterochromia of hair is the presence of more than one distinct color of hair in the same person. A color difference between scalp hair and a mustache or sideburns is not uncommon. Pubic and axillary hair and eyebrows and eyelashes are often darker than scalp hair in a fair-haired person. Rarely, a circumscribed patch of hair of different colors occurs. However, diffuse heterochromia of black and red scalp hair has not been previously reported. We describe a father and son with this condition.

  7. Negative edge plasma currents in the SINP tokamak

    Indian Academy of Sciences (India)

    X-ray flux ejected out from the plasma column, with reduced magnetic fluctuations. We have made use of an IRC and has been able to show that the improvement in confinement properties is observed in the presence of sustained negative currents. These negative cur- rents have been observed to be initiated after the fall of ...

  8. SPECIAL ARTICLE.PM6

    African Journals Online (AJOL)

    QuickSilver

    2003-05-20

    May 20, 2003 ... expect that research exert a significant impact on clinical prac- tice, if clinical practice does not exert a significant ... specific mental disorder and contains several reviews of cur- rently available research evidence ... eating disorders and phobias are in preparation. World Psychiatry is sent free of charge to ...

  9. Download this PDF file

    African Journals Online (AJOL)

    Hepatitis C Virus (HCV) is a significant cause of morbidity and mortality. This infectious disease has spread to more than 200 million people worldwide with steady increases in the number of cases each year1. Despite recent progress, cur- rent therapies (First generation–nonspe- cific) remain inadequate for the majority.

  10. Optimization of the anterior cruciate ligament injury prevention paradigm: novel feedback techniques to enhance motor learning and reduce injury risk

    NARCIS (Netherlands)

    Ariel V. Dowling; Gregory D. Myer; Timothy E. Hewett; Anne Benjaminse; James A. Onate; Alli Gokeler; Avery Faigenbaum; Kevin R. Ford; Bert Otten

    2015-01-01

    Primary anterior cruciate ligament (ACL) injury prevention programs effectively reduce ACL injury risk in the short term. Despite these programs, ACL injury incidence is still high, making it imperative to continue to improve cur- rent prevention strategies. A potential limitation of current ACL

  11. Research Article Special Issue

    African Journals Online (AJOL)

    2017-09-10

    Sep 10, 2017 ... ABSTRACT. This work was carried out at the health ce patients with diseases related to heal cardiovascular related-disease. The aim o visitors and patients to determine the occup the occupants' perception on the cur. Recommendations have been made to impr accepted level of relative humidity, tempera.

  12. Fast Radio Bursts

    Indian Academy of Sciences (India)

    Akshaya Rane

    2017-09-12

    Sep 12, 2017 ... effects observed in large-scale structures and is cur- rently estimated to constitute about ∼26% of the total energy density of the Universe. The remaining ∼5% comprises all ordinary matter that is made of protons and neutrons that make up atoms and we refer to it as the baryonic matter (detectable matter).

  13. State-of-the-art inventory

    NARCIS (Netherlands)

    Verhagen, H.J.; Van Gerven, K.A.J.; Akkerman, G.J.

    2005-01-01

    The present report provides a state-of-the-art inventory of relevant information and technical concepts for the ComCoast project, being the first phase of the research stages of Work Package 3 (WP3). This project was assigned to Royal Haskoning by CUR. The information scan was set-up in a systematic

  14. 51 ST. AUGUSTINE VERSUS NIGERIA ON CORPORATE ...

    African Journals Online (AJOL)

    Tracie1

    Angwe Ken Agede:St. Augusrine Versus Nigeria on Corporate Corruption and impunity… 53 ... and South. Korea are making frantic efforts to curЛ this trend. It ... interest in philosophy and re-oriented his values. In a draft of a correspondence of 371 to his mother. Augustine shares his discovery and contemplates course.

  15. Article

    African Journals Online (AJOL)

    2012-06-15

    Jun 15, 2012 ... cur in alternative forms such as through the use of written and audiovisual educational materials in appropriate languages, or over the telephone, both of which have .... The translation of US testing kits to the. South African context requires consideration of several issues. First, an appropriate test must detect ...

  16. The effect of anticholinergic drugs on 123I-IMP SPECT in Parkinson's disease

    International Nuclear Information System (INIS)

    Mizuno, Tomoyuki; Nishiyama, Kazutoshi; Hitoshi, Seiji; Takeda, Koichi; Sakuta, Manabu

    1992-01-01

    Anticholinergic drugs may be responsible for mental deterioration in Parkinson's disease (PD). This study was thus performed to examine effects of anticholinergic drugs on the brain by using N-isopropyl-p-[I-123] iodoamphetamine SPECT. The purpose of the study was twofold: (I) to compare regional cerebral uptake of tracer during treatment with anticholinergic drugs and one month after the discontinuation of the drugs in 7 PD patients given them for 6 months or more; and (II) to compare tracer uptake in 11 PD patients administered anticholinergic drugs and 25 PD patients not administered them. Each 16 regions in the bilateral cerebral cortexes and each one region in the bilateral basal ganglia, thalamus, and cerebellum were assigned as regions of interest (ROI). The count ratio of each ROI in the cerebrum to ROI in the cerebellum was designated as regional cerebral uptake ratio (rCUR). A mean rCUR was lower during administration of anticholinergic drugs in all ROIs, except for two in Group I and one in Group II, than during the period not administered the drugs. The administration of anticholinergic drugs was sigificantly associated with decreased rCUR in 10 ROIs in Group I, and in 15 ROIs in Group II. The rCUR in the occipital, basal ganglia, and thalamus was independent of the administration of anticholinergic drugs. These results suggest that anticholinergic drugs may inhibit the cortical cholinergic system in PD patients. (N.K.)

  17. Transport properties of poly(GACT)–poly(CTGA) deoxyribonucleic acid

    Indian Academy of Sciences (India)

    Deoxyribonucleic acid; electronic transmission; carbon nanotube; ladder model; Green's function method. PACS Nos 73.63.-b; 73.63.Fg; 81.07.Nb. 1. Introduction. The discovery that the deoxyribonucleic acid (DNA) can conduct the electric cur- rent, has made it an interesting candidate for the roles that nature did not intend.

  18. Uso y abuso de los ansiolíticos y antidepresivos

    OpenAIRE

    Jeremías, Cristina; Sánchez, Estela; Ruiz, Ilda; Galván, Jessica; Roquer, Marc; Zayas, Patricia

    2017-01-01

    Treballs d'Educació Farmacèutica als ciutadans. Unitat Docent d'Estades en Pràctiques Tutelades. Facultat de Farmàcia, Universitat de Barcelona. Curs: 2016-2017. Tutors: Jorge Casas Sánchez, i Marian March Pujol.

  19. Seasonal variation in vitamin D status in elite athletes: a longitudinal study

    NARCIS (Netherlands)

    Backx, E.M.P.; Avoort, van der Cindy; Tieland, C.A.B.; Maase, K.; Kies, Arie; Loon, van L.J.C.; Groot, de C.P.G.M.; Mensink, M.R.

    2017-01-01

    Studies monitoring vitamin D status in athletes are seldom conducted for a period of 12 months or longer, thereby lacking insight into seasonal fluctuations. The objective of the cur-rent study was to identify seasonal changes in total 25-hydroxyvitamin D (25(OH)D) concen-tration throughout the

  20. Capture of interplanetary bodies in geocentric orbits and early lunar ...

    Indian Academy of Sciences (India)

    several orders of magnitude compared to the planetary accumulation era. 1. Introduction. The formation of various bodies in the solar system, starting from the solar nebula, is cur- rently understood in terms of the standard model of planet formation given by Safronov. (1969), Wetherill (1980, 1986) and Wetherill and.

  1. Evolution of Trace Gases and Particles Emitted by a Chaparral Fire in California

    Science.gov (United States)

    2012-02-07

    measurements in BB plumes and concluded that O3 formation is ubiquitous in tropical BB plumes, but O3 formation or destruction can oc- cur in extratropical BB...Fearnside, P. M., Leal Jr., N., and Fernandes, F. M.: Rainforest burning and the global carbon budget: biomass, combustion effi- ciency, and charcoal

  2. Introduced organisms pose the most significant threat to the ...

    African Journals Online (AJOL)

    spamer

    Introduced organisms pose the most significant threat to the conservation status of oceanic islands (e.g.. Williamson 1996). Subantarctic Prince Edward Island, the smaller of the two islands in the Prince Edward. Island group, has few introduced organisms; it is cur- rently known to support only three introduced animals.

  3. La lucha contra el Acoso y el Hostigamiento Sexual: Apuntes para una propuesta de trabajo desde la práctica política de la Diferencia Sexual

    OpenAIRE

    Pineda López, Yolanda

    2014-01-01

    Diploma de Postgrau "La pràctica de la diferència", dins del Màster en Estudis de la Diferència Sexual, Universitat de Barcelona. DUODA, Centre de recerca de dones, curs: 2012-2013, Tutora: Dra. Asun López Carretero

  4. Facile Synthesis of Curcumin-Loaded Starch-Maleate Nanoparticles

    Directory of Open Access Journals (Sweden)

    Suh Cem Pang

    2014-01-01

    Full Text Available We have demonstrated the loading of curcumin onto starch maleate (SM under mild conditions by mixing dissolved curcumin and SM nanoparticles separately in absolute ethanol and ethanol/aqueous (40 : 60 v/v, respectively. Curcumin-loaded starch-maleate (CurSM nanoparticles were subsequently precipitated from a homogeneous mixture of these solutions in absolute ethanol based on the solvent exchange method. TEM analysis indicated that the diameters of CurSM nanoparticles were ranged between 30 nm and 110 nm with a mean diameter of 50 nm. The curcumin loading capacity of SM as a function of loading duration was investigated using the UV-visible spectrophotometer. The loading of curcumin onto SM increased rapidly initially with loading duration, and the curcumin loading capacity of 15 mg/g was reached within 12 hours. CurSM nanoparticles exhibited substantially higher water solubility of 6.0 × 10−2 mg/mL which is about 300 times higher than that of pure curcumin. With enhanced water solubility and bioaccessibility of curcumin, the potential utility of CurSM nanoparticles in various biomedical applications is therefore envisaged.

  5. Investigaciones con el virus del tifo exantemático en nariño

    OpenAIRE

    Groot, Hernando

    2011-01-01

    Estudiando en ciento cincuenta curíes las características del virus del tifo exantemático, aislado de enfermos del departamento de Nariño, hemos visto que estas corresponden a las de un virus de tipo europeo, clásico, no orquítico.

  6. Investigaciones con el virus del tifo exantemático en Nariño

    Directory of Open Access Journals (Sweden)

    Hernando Groot

    1941-10-01

    Full Text Available Estudiando en ciento cincuenta curíes las características del virus del tifo exantemático, aislado de enfermos del departamento de Nariño, hemos visto que estas corresponden a las de un virus de tipo europeo, clásico, no orquítico.

  7. Biodegradable poly(lactic acid) microspheres containing total ...

    Indian Academy of Sciences (India)

    Administrator

    major interest in the microencapsulation process is cur- rently in the application of copying papers, cosmetics, adhesives ... control diffusion of the drug, followed by polymer bio- degradation and elimination of the degradation ... Each batch sample of microshperes was dissolved in dichloromethane and the drug content was ...

  8. Gehalten van dioxinen in boerenkool

    NARCIS (Netherlands)

    Liem AKD; Theelen RMC; Hoogerbrugge R; de Jong APJM; LOC; TOX

    1993-01-01

    This report describes the results of a study on dioxin levels in samples of curly kale, collected in deposition areas of municipal waste incinerators and a metal reclamation plant as well as in background locations in the Netherlands. From earlier reports on environmental contamination with dioxins

  9. Untitled

    African Journals Online (AJOL)

    Ergotamine and ergometrine standard curVEs were ~ drawn from stock solutions{ by dilution with distilled water and ... samples was determined using direct plating of 100 grain kernels or flour on PDA (10). The alkaloids ... curve was drawn by taking the absorbance value of. difi'erent dilutions of standard ergotamine and".

  10. CIFSRF / IRIACC PROFILES

    International Development Research Centre (IDRC) Digital Library (Canada)

    In Phase 1 of the project, researchers developed a heat-stable, single-shot vaccine that can protect sheep and goats from five common diseases. It is cur- rently being fine-tuned for use with cattle. A single-dose vaccine makes it easier for suppliers to streamline production, marketing, and distribution to reach farmers in rural.

  11. Why heaven kissed earth : the christology of Thomas Goodwin (1600-1680)

    NARCIS (Netherlands)

    Jones, Mark

    2009-01-01

    My dissertation, “Why Heaven Kissed Earth”, looks at the Christology of the Reformed theologian and English Puritan, Thomas Goodwin (1600-1680). The thesis attempts to answer a question first posed by St. Anselm of Cantebury, namely, Cur Deus Homo (“Why did God become man?). Goodwin answers that

  12. Two-electron Oxidation of a Twisted Non Anti-aromatic 40π ...

    Indian Academy of Sciences (India)

    Abstract. Expanded isophlorins are typical examples for stable anti-aromatic systems. Paratropic ring cur- rent effects are observed in their NMR spectra mainly due to their planar conformation. Herein we report the synthesis of the first twisted 40π expanded isophlorin and also its two-electron oxidation to a 38π dication.

  13. TREATMENT OF SEVERE PARKINSONISM WITH L-DOPA*

    African Journals Online (AJOL)

    1971-05-08

    May 8, 1971 ... day bears his name in 1817. He had a sceptical attitude towards the medicinal treatment of the disease, but cur- rent research has given hope of providing more effective drug therapy. A fruitful ;;.pproach has been the treatment of parkinsonism by stereotactic surgery but recently, with the advent of L-dopa, ...

  14. Carrier dynamics analysis in metal-semiconductor-metal device for mid-IR silicon photonics

    DEFF Research Database (Denmark)

    Hui, Alvin Tak Lok; Ding, Yunhong; Hu, Hao

    2017-01-01

    A modelling platform for active carrier removal based on metal-semiconductor-metal structure is reported on analysis of carrier dynamics. The analysis reveals electric current hot spots exist in geometric singularities and curly trajectory of carriers should be considered when accurately estimati...... the effective carrier lifetime....

  15. La lectura pot ser un factor de resiliència? L’anaguet Lleig: ell també era un cigne!

    OpenAIRE

    Torta i Reverté, Mercè

    2015-01-01

    Postgrau en Educació Emocional i Benestar, Facultat de Pedagogia, Departament de Mètodes d’Investigació i Diagnòstic en Educació, Universitat de Barcelona, curs: 2009-2010, Tutor/Tutora: Montserrat Deulofeu i Fontanillas

  16. XML Schema of PaGE-OM: page-om.xsd [

    Lifescience Database Archive (English)

    Full Text Available because it is identified by a SimpleIdentifier). Usual evidence sources are BiblioReferences, Studies and Ontology...t unit, e.g. CA ...tation> ogy...curs=0 maxOccurs=unbounded> _term minOccurs=1 maxOccurs=1>

  17. Army Support of Military Cyberspace Operations: Joint Contexts and Global Escalation Implications

    Science.gov (United States)

    2015-01-01

    Alexander as well as its cur- rent operations led by Admiral Michael Rog - ers. Also, it assesses the command’s mission to direct operations, defend...Questions for Vice Admiral Michael S. Rog - ers, USN Nominee for Commander, United States Cyber Com- mand,” Washington, DC: U.S. Senate, March 11

  18. Decoupled Double Synchronous Reference Frame Current Controller for Unbalanced Grid Voltage Conditions

    DEFF Research Database (Denmark)

    Reyes, M.; Rodriguez, Pedro; Vazquez, S.

    2012-01-01

    Due to the growing changes in the electrical network related to the new distributed generation scheme and the integration of renewable energy sources, new requirements for grid-connected power converters are being defined in the new grid codes. The injection of positive- and negative-sequence cur...

  19. Moisture in Crawl Spaces

    Science.gov (United States)

    Anton TenWolde; Samuel V. Glass

    2013-01-01

    Crawl space foundations can be designed and built to avoid moisture problems. In this article we provide a brief overview of crawl spaces with emphasis on the physics of moisture. We review trends that have been observed in the research literature and summarize cur-rent recommendations for moisture control in crawl spaces.

  20. Building a shared understanding of the skills and competences in order to respond to the current global technical challenges

    DEFF Research Database (Denmark)

    Friesel, Anna; Ward, Anthony; Welzer, Tatjana

    2014-01-01

    A pan-European team, including the representatives from 45 European universities, is working on an EU supported project to firstly explore and then provide models for ways in which Higher Education Institutions of Europe in the Electrical and Information Engineering disciplines can respond to cur...

  1. New Vibrio cholerae O1 Biotype ElTor bacteriophages

    Directory of Open Access Journals (Sweden)

    Sen Anindito

    2005-04-01

    Full Text Available Abstract We report the presence of three new O1 ElTor vibriophages named AS1, AS2 and AS3, isolated from the sewage and pond waters of the outskirts of Kolkata. A few phages, named AS4, with hexagonal heads and abnormally long tails with typical curly projections were also found in the water samples.

  2. Analysis of Liquid Rocket Tankage

    Science.gov (United States)

    1975-04-01

    pitting and essencially no attack of’ the base metal. The internal surfacees showed some shallow pitting and discoloration which 28 were less severe than...301 STAINLESS STEEL. spoIclus Cur FROM PARENT KETAL AND W=O JOINTS Oil A2IDE CRYOPORMNT OLTIATZ 0’ .% OFFSET TOS ILE YI AID 5 LOCATION"UNDOU S?1C1M N

  3. Antimicrobial synergy of apple skin polyphenol and thyme essential oil in Açaí edible films and their effects on film properties (abstract)

    Science.gov (United States)

    Introduction: Diseases caused by food borne pathogens are of great concern to the food industry. The consumption of foods with chemical preservatives has led to increased consumer concern and demand for natural and minimally processed foods. In addition, environmental concerns are generated from cur...

  4. Protective Effects of Tetrahydrocurcumin and Curcumin against ...

    African Journals Online (AJOL)

    Purpose: To investigate the cytoprotective effect of tetrahydrocurcumin, (THC) and curcumin (CUR) on cytotoxicity induced by doxorubicin and cadmium in Chang liver cells. Methods: Cytotoxicity was determined by sulforhodamine B assay. The expression of nuclear factorerythroid- 2-related factor 2 (Nrf2) Nrf2 regulated ...

  5. Curcumin and salsalate suppresses colonic inflammation and procarcinogenic signaling in high-fat-fed, azoxymethane-treated mice

    Science.gov (United States)

    High-fat diets (HFDs) and excess adiposity increase proinflammatory cytokines in the colon, altering gene expression in a manner that promotes the development of colorectal cancer (CRC). Thus, compounds that reduce this biochemical inflammation are potential chemopreventive agents. Curcumin (CUR), a...

  6. Threats to the validity of locally developed multiple-choice tests in medical education: construct-irrelevant variance and construct underrepresentation.

    Science.gov (United States)

    Downing, Steven M

    2002-01-01

    Construct-irrelevant variance (CIV) - the erroneous inflation or deflation of test scores due to certain types of uncontrolled or systematic measurement error - and construct underrepresentation (CUR) - the under-sampling of the achievement domain - are discussed as threats to the meaningful interpretation of scores from objective tests developed for local medical education use. Several sources of CIV and CUR are discussed and remedies are suggested. Test score inflation or deflation, due to the systematic measurement error introduced by CIV, may result from poorly crafted test questions, insecure test questions and other types of test irregularities, testwiseness, guessing, and test item bias. Using indefensible passing standards can interact with test scores to produce CIV. Sources of content underrepresentation are associated with tests that are too short to support legitimate inferences to the domain and which are composed of trivial questions written at low-levels of the cognitive domain. "Teaching to the test" is another frequent contributor to CUR in examinations used in medical education. Most sources of CIV and CUR can be controlled or eliminated from the tests used at all levels of medical education, given proper training and support of the faculty who create these important examinations.

  7. Proceedings of the Workshop on Unsteady Separated Flow Held at the United States Air Force Academy on August 10-11, 1983

    Science.gov (United States)

    1984-05-01

    file for further analysis. The procedure abcve is then repeated for each probe position. In this runner , the complete time...Calculation Methods Cur e’s original nethod has been modified to ..j" .. Kite trie jnsteady bpunddry layer dev.e1oprnent. rnis is

  8. A Modular Coassembly Approach to All-In-One Multifunctional Nanoplatform for Synergistic Codelivery of Doxorubicin and Curcumin

    Directory of Open Access Journals (Sweden)

    Muyang Yang

    2018-03-01

    Full Text Available Synergistic combination therapy by integrating chemotherapeutics and chemosensitizers into nanoparticles has demonstrated great potential to reduce side effects, overcome multidrug resistance (MDR, and thus improve therapeutic efficacy. However, with regard to the nanocarriers for multidrug codelivery, it remains a strong challenge to maintain design simplicity, while incorporating the desirable multifunctionalities, such as coloaded high payloads, targeted delivery, hemodynamic stability, and also to ensure low drug leakage before reaching the tumor site, but simultaneously the corelease of drugs in the same cancer cell. Herein, we developed a facile modular coassembly approach to construct an all-in-one multifunctional multidrug delivery system for the synergistic codelivery of doxorubicin (DOX, chemotherapeutic agent and curcumin (CUR, MDR modulator. The acid-cleavable PEGylated polymeric prodrug (DOX-h-PCEC, tumor cell-specific targeting peptide (CRGDK-PEG-PCL, and natural chemosensitizer (CUR were ratiometrically assembled into in one single nanocarrier (CUR/DOX-h-PCEC@CRGDK NPs. The resulting CUR/DOX-h-PCEC@CRGDK NPs exhibited several desirable characteristics, such as efficient and ratiometric drug loading, high hemodynamic stability and low drug leakage, tumor intracellular acid-triggered cleavage, and subsequent intracellular simultaneous drug corelease, which are expected to maximize a synergistic effect of chemotherapy and chemosensitization. Collectively, the multifunctional nanocarrier is feasible for the creation of a robust nanoplatform for targeted multidrug codelivery and efficient MDR modulation.

  9. Guideline for service life design of structural concrete. A performance based approach with regard to chloride induced corrosion

    NARCIS (Netherlands)

    Wegen, G. van der; Polder, R.B.; Breugel, K. van

    2012-01-01

    Nowadays major structures require a service life of 100 years or even more. CUR committee VC81 has developed a guideline for service life design based on initiation of corrosion due to chloride penetration for exposure classes XD or XS. A semi-probabilistic simplification of the DuraCrete

  10. Guideline for service life design of structural concrete : A performance based approach with regard to chloride induced corrosion

    NARCIS (Netherlands)

    Wegen, G. Van der; Polder, R.B.; Breugel, K. Van

    2012-01-01

    Nowadays major structures require a service life of 100 years or even more. CUR committee VC81 has developed a guideline for service life design based on initiation of corrosion due to chloride penetration for exposure classes XD or XS. A semi-probabilistic simplification of the DuraCrete

  11. Protective Effects of Tetrahydrocurcumin and Curcumin against ...

    African Journals Online (AJOL)

    Purpose: To investigate the cytoprotective effect of tetrahydrocurcumin, (THC) and curcumin (CUR) on cytotoxicity induced by doxorubicin and cadmium in Chang liver cells. Methods: Cytotoxicity was determined by sulforhodamine B assay. The expression of nuclear factor- erythroid-2-related factor 2 (Nrf2) Nrf2 regulated ...

  12. AFRRI (Armed Forces Radiobiology Research Institute) Reports, April-June 1985.

    Science.gov (United States)

    1985-01-01

    body (Metcalfe et al., 1981) and is increased gastrointestinal blood flow in dogs released under the stimulus of ionizing radia- " tion (Doyle and...jury models that we have estab- rmyonecrosis Igas gangrene) oc- mechanisms, ie, peristalsis and lished. The canine, because of its curs when

  13. Aspects of the Biology of the Carrot Aphid Chomaphis (dysaphis ...

    African Journals Online (AJOL)

    1958), and often covered with soil. Although C.foeniculus has hitherto not been reported from Uganda, recent observations at. Makerere University Agricultural Research In- stitute, Kabanyolo (MUARIK), suggest that sig- nificant infestation of carrots by the aphid oc- curs in the country. Given this pest potential (a.

  14. Low resistance polycrystalline diamond thin films deposited by hot ...

    Indian Academy of Sciences (India)

    Administrator

    resistivity was investigated using a 4-point probe cur- rent/voltage in van der Pauw (1958) geometry at room temperature with maximum voltage of ± 100 V. The cu- rrent and voltage were measured using a semiconductor device analyser (Agilent B1500A). 3. Results and discussion. To investigate the effect of varying gas ...

  15. JOURN AL OFTHE CAMEROON ACADEMY OFSCIENCES

    African Journals Online (AJOL)

    basic principle is that a strategic planning framework must be customized to fit the needs and resources of an .... The principles, the theories of information handlingare established and taught through the substance of cur- .... former school of journalism where the library/infor- mation science programis housed, gives the ...

  16. Untitled

    African Journals Online (AJOL)

    edge of the ulcer was done using cutting cur- rent. Resection was started from outside the edge of the ulcer (in healthy tissue) towards the centre of the ulcer in a semi-filled bladder. In cases with multiple ulcers, the lower ones were resected before the upper ones. Care was taken to ensure a rapid resection with low.

  17. Biomonitoring of toxic compounds of airborne particulate matter in urban and industriel areas

    DEFF Research Database (Denmark)

    Klumpp, Andreas; Ro-Poulsen, Helge

    2010-01-01

    on man and environment. Two widely applied biomonitoring procedures, namely the standardised ryegrass exposure for monitoring of trace metals, and the standardised exposure of curly kale for monitoring of PAH compounds, is presented taking examples from a Europe-wide biomonitoring study conducted in 11...

  18. clinical

    African Journals Online (AJOL)

    py, histology and a CD4 count every 6 months for 36 months showed ... by CD4 counts. A Finnish study25 of 153 HIV-positive women followed up for a mean of 5.6 years showed. 33% to have cervical neoplasia. The risk of cervical ne- oplasia was not ..... genome and oncogenic gene changes are already oc- curring, ART ...

  19. Pulsed nanocrystalline plasma electrolytic boriding as a novel ...

    Indian Academy of Sciences (India)

    WINTEC

    Abstract. Potentiodynamic polarization and electrochemical impedance spectroscopy were employed to test borided CP-Ti, treated by a relatively new method called pulsed plasma electrolytic boriding. The results show excellent corrosion resistance for modified CP-Ti. The effect of frequency and duty cycle of pulsed cur-.

  20. Isolation and Characterisation of Yeasts and Bacteria From From ...

    African Journals Online (AJOL)

    ," .. normal growth curVe: When the ratio of Aeeto-. ,Haggblade, S. and Holzapfel, W. 1989. Industrialization of baeter to yeast was' 1: 1 very···· iittle growth oc-. AfricaI].~~ .. iI].digenous beer brewing. Page 191-283 \\1}:. K.H.Steinkrans (Ed.).

  1. Analysis of tokamak plasma confinement modes using the fast ...

    Indian Academy of Sciences (India)

    2016-10-20

    Oct 20, 2016 ... without external field (electric biasing), and then compared it with each other. After the Fourier analysis of Mirnov coil data, the ... radial electric field at the plasma edge. It is commonly accepted that improvement of ... The biased electrode drives a radial cur- rent between itself and the vacuum vessel and the.

  2. Vegetative Analysis of the Flookplain of the Trinity River, Texas,

    Science.gov (United States)

    1974-09-30

    Willd. Cupgrass Eriochloa sericea (Scheele) Monro. Curly-cup gumweed Grindelia squarrosa, (Pursh.) Dun. var. squarrosa Cut-leaved evening Oenothera ...Eryngo Eryngium Hookeri Waip. Eryngo Eryngium integrifolium Walt. Evening primrose Oenothera heterophylla Spach. Eyebane Euphorbia nutans Lag. Fall...Heipi helianthoides (L)Sweetj Ozark grass Limnodea arkansana (Nutt.) L. H. DeweyI Palafoxia Palafoxia Reverchonji (Bush) Cory Pulafoxia Palafoxia rosea

  3. A practical solution in delineating thin conducting structures and ...

    Indian Academy of Sciences (India)

    density at various depths for varying current elec- trode separations. The derivation of horizontal cur- rent density is given in Appendix I. In Appendix I, it has been shown that horizontal current density increases ... this level corresponds to the contact between clay and lateritic basement. Since calcareous laterite has very ...

  4. The ergonomics of safe needle disposal following venous cannula ...

    African Journals Online (AJOL)

    QuickSilver

    PRIZE SUBMISSION. Southern African Journal of Anaesthesia & Analgesia - July 2003. 15. The ergonomics of safe needle disposal following venous cannula insertion ... jury per year. Again two studies have demonstrated that 40% of needlesticks occur during the actual procedure and almost 40% of injuries oc- cur during ...

  5. Pegylated niosomal nanoparticles loaded with vincristine ...

    African Journals Online (AJOL)

    lead to autonomic and peripheral sensorimotor neuropathy [3]. To decrease the side effects and simultaneously increase the therapeutic activity of VCR, studies ..... Nanomedicine. 2015; 10: 3081. 4. Swain S, Sahu PK, Beg S, Babu SM. Nanoparticles for cancer targeting: Current and future directions. Cur Drug. Deliv. 2016 ...

  6. Differences between angiotensin-converting enzyme inhibition and angiotensin II-AT(1) antagonism on angiotensin-mediated responses in human internal mammary arteries

    NARCIS (Netherlands)

    Voors, AA; Oosterga, M; Buikema, H; Mariani, M; Grandjean, JG; van Glist, WH

    The cur-rent study aimed to demonstrate differences between angiotensin (Ang)-converting enzyme (ACE) inhibition and Ang II-AT(1) receptor antagonism on full concentration-contraction responses to Ang I. Contraction responses to increasing concentrations of Ang I (1 nM-1 muM) were evaluated in organ

  7. fluoride iontophoresis versus topical fluoride application

    African Journals Online (AJOL)

    The process of influencing ionic motion by electrical cur- rent has been termed iontophoresis, electophoresis ... In iontophroresis, it is believed that fluoride ion is elec- trically driven deeper into the dentinal tubules. ..... the hydraulic conductance of Human Dentin, in vitro, J. Dent. Res. 1981; 60:686698. 6. Gillam D G. Clinical ...

  8. Strengthening Sustainability and Resiliency of a Future Force, Phase 1. FY2010-2011 Summer Study

    Science.gov (United States)

    2011-03-01

    with hasty planning, excessive stockages , little recycling, and limited local (onsite) production. Although this mode of operation is still a...calculated stockage objective in the two theaters, based upon weapons densities, historical planning factors, anticipated consumption data, and the...Growing power and thermal management demands are being added to new and cur- rent vehicles. o More equipment, armor, silent overwatch, and

  9. Defense Systems Management Review. Volume 3, Number 2, Spring 1980. Managing the Process.

    Science.gov (United States)

    1980-01-01

    suffi- cient, resulting in ineffective competition since they would feel no compulsion to be the low bidder. -There is no incentive fer a new company...advantageous to the government. In practice, however, the cur- rent flexibility to make these essentially subjective decisions is difficult to exer- cise. Buyers

  10. Proposed Barge Terminal Expansion, Packer River Terminal, Inc., South St. Paul, Dakota County, Minnesota.

    Science.gov (United States)

    1977-09-01

    evening primrose (Oenothera S biennis), goose-foot (Chenopodium hybridum), curly dock ( Rumex crispus ), and mullen (Verbascum thapsus). 2.38 Area G - Area G...dock ( Rumex sp.), and various grasses (Graminae) most prevalent. Area A would be entirely filled. 2.32 Area B - Area B (Type I wetland) is a dense

  11. Terrestrial Biological Inventory, Hillview Drainage and Levee District, Greene and Scott Counties, Illinois.

    Science.gov (United States)

    1982-01-01

    Field Sorrel) Rumex crispus L. (Curly Dock) PORTtJLACACEAE Portulaca oleracea L. (Purslane) POTAH’OGETOkIACEAB Potaniogeton foliosus Raf. (Leafy Pondveed...Ell. (Dotted Smartweed) Polygonum scandens L. (Climbing False Buckwheat) Polygonum virginianum L. (Virginia Knotweed) A-6 ,U" Rumex acetosella L

  12. Approaches to Revegetate Shorelines at Lake Wallula on the Columbia River, Washington-Orgeon.

    Science.gov (United States)

    1986-01-01

    ofL Xanthiwn spp., Canyza spp., Rumex crispus , Olenothera spp., Salsola kali, big sagebrush (Arteniesia tridentata), and cheatgrass (Bromus tectorum...Rosa nultiflora nutfoars Rtumex crispus curly dock Saqittaria latifolia duck potato Salix exigua slender willow . . - Salix fragilis crack willow

  13. Bel Marin Keys Unit V Expansion of the Hamilton Wetland Restoration Project. General Reevaluation Report. Volume 1

    Science.gov (United States)

    2003-04-01

    cattail (Typha spp.), salt marsh bulrush (Scirpus maritimus), and curly dock ( Rumex crispus ). Seasonal wetlands commonly provide high tide refugia...dock ( Rumex crispus ). Additionally, non-native grasses such as Mediterranean barley (Hordeum marinum) and perennial ryegrass (Lolium perenne) are

  14. Dual function of the McaS small RNA in controlling biofilm formation

    DEFF Research Database (Denmark)

    Jørgensen, Mikkel Girke; Thomason, Maureen K.; Havelund, Johannes

    2013-01-01

    -pairing and protein titration. Previous work established that McaS base pairs with the mRNAs encoding master transcription regulators of curli and flagella synthesis, respectively, resulting in down-regulation and up-regulation of these important cell surface structures. In this study, we demonstrate that Mca...

  15. Future of European Financial Supervision, Towards a European System of Financial Supervisors

    NARCIS (Netherlands)

    Arons, T.M.C.

    The 2008 financial crisis made clear the shortcomings in the European structure of financial supervision. In the cur­rent system of financial supervision the financial supervi­sor of the home Member State is in principle the only autho­rity entitled to supervise financial institutions even in case

  16. Hacia la conquista de la serenidad

    OpenAIRE

    Rodríguez Neira, Verònica

    2015-01-01

    Postgrau en Educació Emocional i Benestar, Facultat de Pedagogia, Departament de Mètodes d’Investigació i Diagnòstic en Educació, Universitat de Barcelona, curs: 2009-2010, Tutor/Tutora: Esther García Navarro

  17. Potential effects of curcumin on peroxisome proliferator-activated receptor-gamma in vitro and in vivo

    Science.gov (United States)

    Natural peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonists are found in food and may be important for health through their anti-inflammatory properties. Curcumin (Cur) is a bright yellow spice, derived from the rhizome of Curcuma longa Linn. It has been shown to have many biologi...

  18. Proyecto de Alfabetización emocional. La felicidad como actitud. Higiene mental

    OpenAIRE

    Fañanás Ferrer, Goretti

    2013-01-01

    Màster en Educació Emocional i Benestar, Facultat de Pedagogia, Departament de Mètodes d’Investigació i Diagnòstic en Educació, Universitat de Barcelona, curs: 2010-2011, Tutor/Tutora: Esther García Navarro

  19. Análisis del artículo 16.2 de la Ley de Competencia Desleal. (Supuesto práctico Mercadona)

    OpenAIRE

    Sánchez Güell, Roger

    2017-01-01

    Treballs Finals del Doble Grau d'Administració i Direcció d'Empreses i de Dret, Facultat d'Economia i Empresa i Facultat de Dret, Universitat de Barcelona, Curs: 2016-2017 , Tutor: Silvia Gómez Trinidad

  20. A flexible low-voltage ride-through operation for the distributed generation converters

    DEFF Research Database (Denmark)

    Chen, Hsin-Chih; Lee, Chia-Tse; Cheng, Po-Tai

    2013-01-01

    With more and more distributed energy resources (DERs) are installed in the utility grid, the utility requires the DER generation system to remain grid-connected and injects reactive and active power to support grid voltage during voltage sags. In this paper, a positive- and negative-sequence cur...

  1. The perceived roles and functions of school science subject advisors

    African Journals Online (AJOL)

    revisited (Chisholm, 2000) the principles of outcomes-based education still serve as ... chers, field and administrative work, identification of good teachers, communicating and establishing good relations with teachers and cur- riculum development. This positive development occurred at the end of the apartheid era in South ...

  2. Single Assignment C (SAC): High Productivity meets High Performance

    NARCIS (Netherlands)

    Grelck, C.; Zsók, V.; Horváth, Z.; Plasmeijer, R.

    2012-01-01

    We present the ins and outs of the purely functional, data parallel programming language SaC (Single Assignment C). SaC defines state- and side-effect-free semantics on top of a syntax resembling that of imperative languages like C/C++/C# or Java: functional programming with curly brackets. In

  3. NJP VOLUME 41 NO 4

    African Journals Online (AJOL)

    PROF. EZECHUKWU

    2014-04-13

    Apr 13, 2014 ... our children. The aim of the cur- rent study was to compare the ade- quacy of formula methods of weight and height estimation with measured values in children. Materials and Methods: This was a comparative observational study. Children who met the inclusion criteria were selected consecutively.

  4. s~uth africa a~[) i~()erial ~aval [)efe~ce

    African Journals Online (AJOL)

    This trade had been disrupted by the rise of Islam however, and for many years Venice alone managed to se-. CUrE)the spices filtering through the markets ..... guese, they had found the maintenance of a world-wide commercial system an increasing drain on their limited resources and man- power. _The Netherlands.

  5. Pulmonary alveolar proteinosis — a case report and review

    African Journals Online (AJOL)

    Enrique

    with granular, eosinophilic, proteina- ceous material (Fig. 2) with preserva- tion of the alveolar architecture. The patient was treated by whole-lung lavage, followed by a 4-week trial of subcutaneous granulocyte-macro- phage colony-stimulating factor. (GM-CSF). The trial of GM-CSF failed, however, and the patient is cur-.

  6. Cr(VI) generation during sample preparation of solid samples – A ...

    African Journals Online (AJOL)

    2009-05-15

    May 15, 2009 ... mines and the local environment. None of these mines cur- rently make any provision for water-soluble Cr(VI) leaching from their ores. The beneficiation processes employed (e.g. spiral concentration) are water-intensive and would contribute to the mobilisation of soluble hazardous compounds, if present.

  7. Pilot del projecte "L'evolució del vestuari al llarg de la història" : Un projecte interdisciplinari amb l'ús de les TIC per a 6è de primària

    OpenAIRE

    Salvador Galí, Xavier

    2013-01-01

    Projecte interdisciplinari per a 6è curs de primària amb l'ús de les TIC. Proyecto interdisciplinario para 6º curso de primaria con el uso de las TIC. Master thesis for the Education and ICT (e-learning) program.

  8. Classroom

    Indian Academy of Sciences (India)

    Transition to Turbulence in a Smoke Jet: A vertical smoke jet was created by burning incense. To reduce the effect of air cur- rents in the vicinity of the jet, the burning incense was covered on three sides by a piece of cardboard (with black paper pasted on it for contrast). A video of the resulting KH instability was taken.

  9. lessons from fly models

    Indian Academy of Sciences (India)

    1991) and spinal and bulbar muscular atrophy (SBMA; Laspada et al. 1991). Cur- rently, about 20 such disorders are known, nine of which are neurodegenerative and result from expansion of CAG re- peats coding for polyglutamine (polyQ) tracts. Among these,. Huntington's disease (HD) and Machado–Joseph disease.

  10. 1H-indoles: Synthesis, SAR and biological evaluation as a novel ...

    Indian Academy of Sciences (India)

    impairment; structure activity relationship; cross selectivity; pharmacokinetic profile. 1. Introduction. Cognitive decline is a defining symptom of many severe neurological disorders, viz., Alzheimer's disease (AD),. Schizophrenia, Parkinson's disease (PD), etc.1 The cur- rent options to treat cognitive symptoms associated with.

  11. Genètica en embriologia humana

    OpenAIRE

    Megías Vericat, Javier

    2015-01-01

    Aquests materials han rebut un incentiu del Servei de Política Lingüística. Diapositives de les classes de l'assignatura d'Embriologia del grau de Medicina. Grup de valencià. Curs 2014-15. Slides from the subject of Embriology. Degree of Medicine. 2014-2015.

  12. Worldwide Crisis Alerting Network, Phase II. Task 2. Identification of Existing Communications Systems,

    Science.gov (United States)

    1980-04-01

    MARIA RTF SONORE STROMFJORD LTF With drop at: Avec dfrivation 9: GOOSE ICon derivaci6n en: HABANA ACC HOUSTON ACC LTF Through/via/a traves de: MIAMI...1) [(2) (3) (4) (5) (6) Muscat Oman NOT BASHBY Nepal Km1~ Netherlands all M Netherlamms Antilles AUR IB KL L4 PR VA CUR NYC BON all other airlines PrP

  13. Fluir en la escuela. Una aproximación a la experiencia óptima desde la educación emocional

    OpenAIRE

    Sánchez Orpella, Verónica

    2015-01-01

    Postgrau en Educació Emocional i Benestar, Facultat de Pedagogia, Departament de Mètodes d’Investigació i Diagnòstic en Educació, Universitat de Barcelona, curs: 2014-2015, Tutor/Tutora: Manuel Álvarez

  14. Shunt hybrid active power filter for harmonic mitigation: A practical ...

    Indian Academy of Sciences (India)

    In summary since PPF is not a cur- rent controlled system, the amount and order of harmonics sinking/sourcing is uncontrollable. To overcome these shortcomings of PPF, various Active Power Filter (APF) configurations have been reported. At the same time, the following problems of shunt APF have been pointed out.

  15. YL-SUBSTITUTED PHENOLS AND THEIR POTENTIAL ...

    African Journals Online (AJOL)

    nb

    Based on Cashew Nut Shell Liquid. (CNSL). Int. J. Cur. Res. 2: 285-292. Nagia MMS, El-Metwally MM, Shaaban M,. El-Zalabani SM and Hanna AG. 2012 Four Butyrolactones and. Diverse. Bioactive. Secondary. Metabolites from. Terrestrial. Aspergillus flavipes MM2: Isolation and Structural Determination. Org. Med. Chem.

  16. ONR Far East Scientific Information Bulletin. Volume 14, Number 4, October-December 1989

    Science.gov (United States)

    1989-12-01

    laboratory is cur- Parthenon , allows the design of an LSI circuit rently involved in research in social impacts, to be expressed in a high-level...facilities and integrated-circuit design tool Parthenon , had a short discussion with a couple of developed by Mr. Yukihiro Nakamura and researchers working

  17. Clinical Guidelines for Stellate Ganglion Block to Treat Anxiety Associated With Posttraumatic Stress Disorder

    Science.gov (United States)

    2015-07-01

    certified pain medicine physicians, and is widely available at many hospitals and interventional pain clinics. All cur- rent indications for SGB...the level of the provider’s chest usually facilitates proper ergonomics . The depth of the ultra- sound unit is set to visualize the ventral border of

  18. Original Research Article Effect of Curing Time on pH and Time ...

    African Journals Online (AJOL)

    Erah

    osmotic pressure gradient across the polymeric coating upon contact with dissolution medium.8,9. Marked difference between the curred and uncured dissolution profile was revealed in. Kollicoat SR 30D coated non-pareil-based system.11 Curing the pellets resulted in release profiles, which did not change during.

  19. Fulltext PDF

    Indian Academy of Sciences (India)

    Unknown

    New challenges in human genetics: modifier genes ... and humans. Two well recognized causes for variable phenotypes are the presence of alternative alleles and physiological interaction of environmental factors. ... van Straaten H W and Copp A J 2001 Curly tail: a 50-year history of the mouse spina bifida model; Anat.

  20. Antioxidant Potential of ulva rigida c. Agardh Extract: Protection from Oxidative Stress Hypothyroidism

    Directory of Open Access Journals (Sweden)

    S. TAŞ

    2014-06-01

    Full Text Available The purpose of this study was to evaluate the effects of Ulva rigida C. Agardh, one of the green algae, on and antioxidative system in the propylthiouracil (PTU-induced hypothyroid rats. Thirty-two rats randomly divided into four groups: control (C, control+U. rigida extract (C+UR, hypothyroid (H and hypothyroid+U. rigida extract (H+UR. U. rigida (2% was administered in drinking water for 5 weeks after the induction of hypothyroidism. Hypothyroid rats were under oxidative stress as reflected by icreased plasma and tissue malondialdehyde (MDA levels. U. rigida reduced serum total cholesterol and,- triglyceride levels and plasma and heart skeletal muscle, liver and,- kidney tissue MDA levels in the H+UR group. Serum total cholesterol and tissues MDA levels were reduced in the C+UR group. Whole blood glutathione peroxidase and erythrocyte superoxide dismutase activities were increased in the H+UR and C+UR groups compared with those of te respective control groups. Paraoxonase and arylesterase activities were lower in the H group and U. rigida increased paraoxonase and arylesterase activities in C+UR and H+UR groups. We conclude that hypothyroidism is associated with oxidative stress and, U. rigida extract might have a potential use as a protective antioxidant agent in hypothroidism.

  1. Theory for electrochemical impedance spectroscopy of ...

    Indian Academy of Sciences (India)

    Shweta Dhillon

    having high mean with large variance in the RCT and cd can behave same as an electrode having low mean with small variance in the RCT and .... component of the current density, i0 is the exchange cur- rent density and is related to RCT , i0 ...... Interfaces: Finite Charge Transfer Rates J. Electroanal. Chem. 396 285. 58.

  2. Download this PDF file

    African Journals Online (AJOL)

    The wavelength of exciting light used in the determination of fluorescence spectra was 410 nm. The emission wavelength for CUR excitation was 500 nm. To remove stray light, sharp cut-off .... difference between the values in ethanol and acetonitrile is because the different solvent media are known to affect electronically ...

  3. Zur Logik des Christentums : eine philosophische Grundlegung ökumenischen Denkens im Ausgang von Anselm von Canterbury

    NARCIS (Netherlands)

    Göbel, Christian

    2012-01-01

    Highlighting the (often ignored) link between "Cur Deus homo" and "Proslogion", the thesis examines the ‘logic of Christianity’ Anselm of Canterbury seeks to demonstrate in an inter-religious context; his atonement theory is not necessarily a result of an ill-conceived religious image of God but

  4. Recent advances in nanoformulations for co-delivery of curcumin and chemotherapeutic drugs

    Directory of Open Access Journals (Sweden)

    Maryam Hashemi

    2017-01-01

    Full Text Available The application of chemotherapy in cancer treatment has been limited due to cause side effects such as toxicity against normal cells and drug resistance. In recent years, numerous studies have been focused on using natural products with chemotherapeutic drugs to enhance therapeutic efficiency and reduce cytotoxicity. On the other hand, encapsulation of drugs into nanoparticles (NPs can improve solubility of hydrophobic drug; circulation time in blood and the residence at the pathological site by enhance permeation and retention (EPR effect. It has been shown that curcumin (CUR has  wide range of pharmacological activities against many diseases such as cancer. CUR has been demonstrated to be a potent chemosensitizer that can induce additive or synergistic effects with chemotherapeutic drugs against different cancer cell lines.  Recently, various types of nanocarriers have been investigated for CUR.  In this review, different co-formulations containing Cur and chemotherapeutic drugs used in cancer therapy are discussed with emphasis on their pharmaceutical properties.

  5. Programa experimental de recitació i cant en cursa (EFDI21 145)

    OpenAIRE

    Galera, Antonio D.

    2015-01-01

    Treball acadèmic per a l'assignatura: Educació Física i la seva didàctica I. Curs: 2014‐15. Resultats de la recerca sobre activitats associades a la cursa econòmica per al desenvolupament de la resistència aeròbica a les edats escolars.

  6. Manganese-mediated ferromagnetism in La2 Mn2x O6 perovskite ...

    Indian Academy of Sciences (India)

    Administrator

    Introduction. Among the ferromagnetic perovskite oxides of cur- rent interest,. 1. La2NiMnO6 is an attractive material that exhibits ferromagnetism near room temperature. (TC ~ 280 K). 2. The magnetism of this oxide is regar- ded as a typical manifestation of the celebrated. Goodenough–Kanamori (G–K) rule,. 3 where the su ...

  7. Design of a CMOS PFD-CP module for a PLL

    Indian Academy of Sciences (India)

    MS received 3 September 2014; revised 23 January 2015; accepted 4 February 2015. Abstract. This paper introduces a modified design of Phase frequency detector. (PFD) with reduced dead zone and improved charge pump (CP) with reduced cur- rent mismatch for a Phase Locked Loop (PLL). Three modified PFD circuits ...

  8. Is the current theory of construction a hindrance to innovation?

    NARCIS (Netherlands)

    Koskela, L.; Vrijhoef, R.

    2001-01-01

    An explanation for the low innovation activity in const¡uction is put forward. The central argument is that the cur¡ent theory of construction is one root cause for low innovation activity. Instead, an explicit and more powerful theory of construction is needed for further imovation, which is 'to

  9. Validation of PPP1R12B as a candidate gene for childhood asthma ...

    Indian Academy of Sciences (India)

    serum IgE levels; however, only a few of them were repli- cated in independent studies (Akhabir and Sandford 2011). Recently, a systematic survey of 117 previously ... PPP1R12B gene; however, only 26 of them passed geno- typing quality criteria in the GABRIEL study. For the cur- rent study, the genotypes for these 26 ...

  10. Protective effects of silymarin and curcumin on cyclophosphamide-induced cardiotoxicity.

    Science.gov (United States)

    Avci, H; Epikmen, E T; Ipek, E; Tunca, R; Birincioglu, S S; Akşit, H; Sekkin, S; Akkoç, A N; Boyacioglu, M

    2017-06-14

    Cyclophosphamide (CP) is a potent anticancer agent; its clinical use is limited due to its marked cardiotoxicity. The present study was aimed at evaluating the cardioprotective effects of silymarin (SLY) and curcumin (CUR), which have strong antioxidant properties, against the toxic effects of high-dose CP on the heart of rats. A total of 36 adult Wistar albino female rats were randomly divided into six groups. Group I (control group; nothing was administered), Group II (CP group; 30mg/kg/day CP was administered intraperitoneally to each animal for seven days), Group III (SLY group; 100mg/kg/day SLY by gavage for 14 days), Group IV (CUR group; 100mg/kg/day CUR by gavage for 14 days), Group V (SLY+CP group; 100mg/kg/day SLY by gavage for 14days plus 30mg/kg/day CP intraperitoneally starting from the seventh day) and Group VI (CUR+CP group; 100mg/kg/day CUR by gavage for 14days plus 30mg/kg/day CP intraperitoneally starting from the seventh day). Biochemical, histopathological and immunohistochemical methods were utilised for evaluation of the cardiotoxicity. The result showed that an increase in heart MDA and DNA fragmentation levels were detected while significant decreases were seen in SOD levels in CP alone group when compared to the other groups. CP caused severe damage in the histopathological status of heart tissue including intersititial oedema, haemorrhage, degeneration and necrosis in muscle fibrils and perinuclear vacuolization. A significant increase in the percentage of TUNEL-positive cells and γH2AX protein expression was detected in the CP-treated group compared to the control and other treated groups. There was significant increase in the percentage of caspase 3-positive cells and decrease in the percentage of Bcl-2 positive cells in the CP group compared to the control group and other treated groups. However, a significant decrease in the percentage of cTnI and cTnT immunoreactivity was also observed in the CP-treated group compared to the control

  11. Thiol Starvation induces redox-mediated dysregulation ofEscherichia colibiofilm components.

    Science.gov (United States)

    Hufnagel, David A; Price, Janet E; Stephenson, Rachel E; Kelley, Jesse; Benoit, Matthew F; Chapman, Matthew R

    2017-10-16

    A hallmark of bacterial biofilms is the production of an e xtra c ellular m atrix (ECM) that encases and protects the community from environmental stressors. Biofilm formation is an integral portion of the u ro p athogenic E scherichia c oli (UPEC) lifecycle. Approximately 2% of UPEC are cysteine auxotrophs. Here, we investigated how cysteine homeostasis impacted UPEC UTI89 strain biofilm formation and, specifically, the production of the ECM components curli and cellulose. Cysteine auxotrophs produced less cellulose and slightly more curli compared to wildtype (WT) strains, and cysteine auxotrophs formed smooth, non-rugose colonies. Cellulose production was restored in cysteine auxotrophs when YfiR was inactivated. YfiR is a redox-sensitive regulator of the diguanylate cyclase, YfiN. Curli production, a temperature-regulated appendage, was independent of temperature in UTI89 cysteine auxotrophs. In a screen of UPEC isolates, we found that ∼60% of UPEC cysteine auxotrophs produced curli at 37°C, but only ∼2% of cysteine prototrophic UPEC produced curli at 37°C. Interestingly, sub-lethal concentrations of mecillinam and trimethoprim/sulfamethoxazole inhibited curli production, whereas strains auxotrophic for cysteine continued to produce curli even in the presence of mecillinam and trimethoprim/sulfamethoxazole. The dysregulation of ECM components and resistance to mecillinam in cysteine auxotrophs may be linked to hyper-oxidation, since the addition of exogenous cysteine or glutathione restored WT biofilm phenotypes to strains unable to produce cysteine and glutathione. Importance Uropathogenic Escherichia coli (UPEC) are the predominant causative agent of urinary tract infections (UTIs). UTIs account for billions of dollars of financial burden to the healthcare industry in the United States annually. Biofilms are an important aspect of the UPEC pathogenesis cascade and for the establishment of chronic infections. Approximately 2% of UPEC strains isolated

  12. Effects of curcumin on the social behavior, blood composition, reproductive hormones in plasma and brain acetylcholinesterase in cadmium intoxicated mice.

    Science.gov (United States)

    Abu-Taweel, Gasem Mohammad

    2016-03-01

    Cadmium (Cd) exposure can induce acute lethal health-related threats in humans since it has an exceptional ability to accumulate in living organism tissues and cause toxicological effects. Curcumin (Cur) on the other hand has a wide variety of biological activities and several studies have suggested its potential therapeutic or protective effects against several ailments and infections. To study the effect of Cur on the toxicity of Cd, Swiss-Webster strain male and female mice (sixty each) were divided into 6 groups of ten each at random. Group-1 served as the naïve control and received no treatment. Group-2, 3 and 4 were the experimental controls and were administered once a day with a single oral dose of 50% dimethyl sulfoxide (DMSO), Cur (300 mg/kg) or Cd (100 mg/kg) respectively, for 2 weeks. Group-5 and 6 received Cur and Cd in combination once a day orally for 2 weeks except that Cur in a dose of 150 and 300 mg/kg to group 5 and 6 respectively, was administered one hour before Cd administration to both groups. After treatment period, the male animals were subjected to social standard opponent test and females were subjected to the tube restraint tests and thereafter, their blood was collected to measure the blood composition indices and level of reproductive hormones. The animals were sacrificed to collect their brain for the estimation of acetylcholinesterase (AChE). Results indicated that Cd significantly increased nonsocial activities in males and latency to first bite in females, whereas the social activities in males and the number of bites in females were significantly decreased. All measured indices of blood composition and levels of progesterone (female) and testosterone (male) in blood and AChE in their brain tissues were significantly decreased due to Cd treatment. However, administration of Cur along with Cd had an ameliorating effect on all the behavioral and biochemical parameters studied herein and reduced the toxicity of Cd significantly

  13. Codelivery of SH-aspirin and curcumin by mPEG-PLGA nanoparticles enhanced antitumor activity by inducing mitochondrial apoptosis

    Directory of Open Access Journals (Sweden)

    Zhou L

    2015-08-01

    Full Text Available Lin Zhou,1,2,* Xingmei Duan,1,2,* Shi Zeng,1 Ke Men,1 Xueyan Zhang,1 Li Yang,1 Xiang Li1 1State Key Laboratory of Biotherapy, Cancer Center and Department of Urology, West China Hospital, Sichuan University, Chengdu, People’s Republic of China; 2Sichuan Food and Drug Safety Monitoring and Review of Certification, Adverse Reaction Monitoring Center, Drug Abuse Monitoring Center, Chengdu, People’s Republic of China *These authors contributed equally to this work Abstract: Natural product curcumin (Cur and H2S-releasing prodrug SH-aspirin (SH-ASA are potential anticancer agents with diverse mechanisms, but their clinical application prospects are restricted by hydrophobicity and limited efficiency. In this work, we coencapsulated SH-ASA and Cur into methoxy poly(ethylene glycol-poly (lactide-coglycolide (mPEG-PLGA nanoparticles through a modified oil-in-water single-emulsion solvent evaporation process. The prepared SH-ASA/Cur-coloaded mPEG-PLGA nanoparticles had a mean particle size of 122.3±6.8 nm and were monodispersed (polydispersity index =0.179±0.016 in water, with high drug-loading capacity and stability. Intriguingly, by treating with SH-ASA/Cur-coloaded mPEG-PLGA nanoparticles, obvious synergistic anticancer effects on ES-2 and SKOV3 human ovarian carcinoma cells were observed in vitro, and activation of the mitochondrial apoptosis pathway was indicated. Our results demonstrated that SH-ASA/Cur-coloaded mPEG-PLGA nanoparticles could have potential clinical advantages for the treatment of ovarian cancer. Keywords: drug delivery, cancer therapy, ovarian cancer, synergistic effect

  14. Combination of arabinogalactan and curcumin induces apoptosis in breast cancer cells in vitro and inhibits tumor growth via overexpression of p53 level in vivo.

    Science.gov (United States)

    Moghtaderi, Hassan; Sepehri, Houri; Attari, Farnoosh

    2017-04-01

    Increased mortality associated with breast cancer in women has spurred the studies to develop new drugs. Arabinogalactan (AG) and curcumin (Cur) are two natural products broadly explored in cancer therapy. Our major goal in the current study was to assess anticancer properties of combination these reagents in vitro on human breast cancer cells and in vivo utilizing animal model of breast cancer. We evaluated cell proliferation, apoptosis, cell cycle, and protein expression in vitro on MDA-MB-231 human breast cancer cells. For in vivo studies, murine breast cancer cells were implanted into BALB/c mice. Thereafter, volume of the developing tumor was calculated and expression of Ki67 and p53 proteins was evaluated to analyze cell proliferation and apoptosis. Combination of AG and Cur significantly decreased cell growth in human breast cancer cells without any significant effect on normal cell growth. This combination could increase cell population in sub-G1 phase, which was indicative of apoptosis. Western blotting showed that the combination of AG and Cur significantly increased Bax/Bcl2 ratio as well as cleaved-caspase3 level in MDA-MB-231 cells. Combination of AG and Cur promoted apoptosis by increasing ROS level, changing mitochondrial membrane and reduction of glutathione. In addition, in vivo studies in mouse showed that this combination could inhibit the progression of breast tumors through over-expression of p53 and reduction of Ki67 levels. Our findings suggest that the combination of AG and Cur is of great potential to induce apoptosis in breast cancer cells in vitro and in vivo. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  15. Cypermethrin-induced histopathological, ultrastructural and biochemical changes in liver of albino rats: The protective role of propolis and curcumin

    Directory of Open Access Journals (Sweden)

    Manal Abdul-Hamid

    2017-06-01

    Full Text Available Cypermethrin (CYP, an insecticide belongs to a synthetic pyrethroid, is used for agriculture and household applications. The present study aimed to examine the toxic effects of CYP on rat liver and to clarify the hepatoprotective effects of propolis (PRO and curcumin (CUR against CYP. This study was assessed in male albino rats, each weighting (120–150 g. The rats were equally divided into six groups as follow; the 1st control group, 2nd PRO group (100 mg/kg/day and 3rd CUR group (100 mg/kg/day. While 4th, 5th and 6th groups were orally treated with CYP (30 mg/kg/day, CYP plus PRO and CYP plus CUR, respectively for 28 days. The present study revealed that CYP-induced significant increase in hepatic markers enzymes (ALT, AST and ALP and elevation in MDA concomitant with significant decrease of SOD and GPx levels. Histological and histochemical results revealed extensive vacuolar degeneration of hepatocytes, fatty change, blood vessel congestion and fibrosis in the liver of CYP- treated group and depletion of glycogen, protein and DNA. Moreover, ultrastructural observations showed vacuolation, damage of mitochondria and nuclear changes. On the other hand, treatment with PRO and CUR led to an obvious improvement of the injured liver tissues and ameliorating the damaging effects of CYP. In conclusion, PRO is markedly effective than CUR in protecting rats against CYP-induced histopathological, ultrastructural and biochemical changes. This protection may be due to its antioxidant properties and scavenging abilities against active free radicals.

  16. Hha controls Escherichia coli O157:H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD.

    Science.gov (United States)

    Sharma, Vijay K; Bearson, Bradley L

    2013-04-01

    Although molecular mechanisms promoting adherence of enterohemorrhagic Escherichia coli (EHEC) O157:H7 on epithelial cells are well characterized, regulatory mechanisms controlling biofilm formation are not fully understood. In this study, we demonstrate that biofilm formation in EHEC O157:H7 strain 86-24 is highly repressed compared to that in an isogenic hha mutant. The hha mutant produced large quantities of biofilm compared to the wild-type strain at 30°C and 37°C. Complementation of the hha mutant reduced the level of biofilm formation to that of the wild-type strain, indicating that Hha is a negative regulator of biofilm production. While swimming motility and expression of the flagellar gene fliC were significantly reduced, the expression of csgA (encoding curlin of curli fimbriae) and the ability to bind Congo red were significantly enhanced. The expression of both fliC and csgA and the phenotypes of motility and curli production affected by these two genes, respectively, were restored to wild-type levels in the complemented hha mutant. The csgA deletion abolished biofilm formation in the hha mutant and wild-type strain, and csgA complementation restored biofilm formation to these strains, indicating the importance of csgA and curli in biofilm formation. The regulatory effects of Hha on flagellar and curli gene expression appear to occur via the induction and repression of FlhDC and CsgD, as demonstrated by reduced flhD and increased csgD transcription in the hha mutant, respectively. In gel shift assays Hha interacted with flhDC and csgD promoters. In conclusion, Hha regulates biofilm formation in EHEC O157:H7 by differential regulation of FlhDC and CsgD, the global regulators of motility and curli production, respectively.

  17. Dietary curcumin supplementation counteracts reduction in levels of molecules involved in energy homeostasis after brain trauma.

    Science.gov (United States)

    Sharma, S; Zhuang, Y; Ying, Z; Wu, A; Gomez-Pinilla, F

    2009-07-21

    Traumatic brain injury (TBI) is followed by an energy crisis that compromises the capacity of the brain to cope with challenges, and often reduces cognitive ability. New research indicates that events that regulate energy homeostasis crucially impact synaptic function and this can compromise the capacity of the brain to respond to challenges during the acute and chronic phases of TBI. The goal of the present study is to determine the influence of the phenolic yellow curry pigment curcumin on molecular systems involved with the monitoring, balance, and transduction of cellular energy, in the hippocampus of animals exposed to mild fluid percussion injury (FPI). Young adult rats were exposed to a regular diet (RD) without or with 500 ppm curcumin (Cur) for four weeks, before an FPI was performed. The rats were assigned to four groups: RD/Sham, Cur/Sham, RD/FPI, and Cur/FPI. We found that FPI decreased the levels of AMP-activated protein kinase (AMPK), ubiquitous mitochondrial creatine kinase (uMtCK) and cytochrome c oxidase II (COX-II) in RD/FPI rats as compared to the RD/sham rats. The curcumin diet counteracted the effects of FPI and elevated the levels of AMPK, uMtCK, COX-II in Cur/FPI rats as compared to RD/sham rats. In addition, in the Cur/sham rats, AMPK and uMtCK increased compared to the RD/sham. Results show the potential of curcumin to regulate molecules involved in energy homeostasis following TBI. These studies may foster a new line of therapeutic treatments for TBI patients by endogenous upregulation of molecules important for functional recovery.

  18. Polyphenols Sensitization Potentiates Susceptibility of MCF-7 and MDA MB-231 Cells to Centchroman

    Science.gov (United States)

    Singh, Neetu; Zaidi, Deeba; Shyam, Hari; Sharma, Ramesh; Balapure, Anil Kumar

    2012-01-01

    Polyphenols as “sensitizers” together with cytotoxic drugs as “inducers” cooperate to trigger apoptosis in various cancer cells. Hence, their combination having similar mode of mechanism may be a novel approach to enhance the efficacy of inducers. Additionally, this will also enable to achieve the physiological concentrations facilitating significant increase in the activity at concentrations which the compound can individually provide. Here we propose that polyphenols (Resveratrol (RES) and Curcumin (CUR)) pre-treatment may sensitize MCF-7/MDA MB-231 (Human Breast Cancer Cells, HBCCs) to Centchroman (CC, antineoplastic agent). 6 h pre-treated cells with 10 µM RES/CUR and 100 µM RES/30 µM CUR doses, followed by 10 µM CC for 18 h were investigated for Ser-167 ER-phosphorylation, cell cycle arrest, redox homeostasis, stress activated protein kinase (SAPKs: JNK and p38 MAPK) pathways and downstream apoptosis effectors. Low dose RES/CUR enhances the CC action through ROS mediated JNK/p38 as well as mitochondrial pathway in MCF-7 cells. However, RES/CUR sensitization enhanced apoptosis in p53 mutant MDA MB-231 cells without/with involvement of ROS mediated JNK/p38 adjunct to Caspase-9. Contrarily, through high dose sensitization in CC treated cells, the parameters remained unaltered as in polyphenols alone. We conclude that differential sensitization of HBCCs with low dose polyphenol augments apoptotic efficacy of CC. This may offer a novel approach to achieve enhanced action of CC with concomitant reduction of side effects enabling improved management of hormone-dependent breast cancer. PMID:22768036

  19. Lyophilized sponges loaded with curcumin solid lipid nanoparticles for buccal delivery: Development and characterization.

    Science.gov (United States)

    Hazzah, Heba A; Farid, Ragwa M; Nasra, Maha M A; El-Massik, Magda A; Abdallah, Ossama Y

    2015-08-15

    This study aimed to prepare and evaluate mucoadhesive sponges as dosage forms for delivering solid lipid nanoparticles. For this purpose curcumin (Cur) was formulated as solid nanoparticles (SLN) using Gelucire 50/13, and polaxomer 407. The prepared CurSLN dispersion was thickened with different mucoadhesive polymers. Different concentrations of glycerol, and mannitol of range (0.25-20%), and (0-1%), respectively were also examined. The formed gel was poured into oblong molds and freeze dried to form mucoadhesive sponge to be applied to the buccal mucosa. The prepared sponges were evaluated for their, in-vivo residence time, in-vitro and in-vivo drug release, and hydration capacity. Surface morphology for the different sponges were examined using SEM. TEM was also carried out for sponge fragments previously dispersed into water. Infrared spectroscopy was conducted to investigate interaction between used ingredients. The results showed that the CurSLN loaded HPMC, and Polycarbophil sponges showed 4, and 15 h in-vivo residence time, respectively, providing a considerable amount of curcumin into saliva. The incorporation of glycerol and mannitol at concentration of 1% provided elegant and flexible sponges. The SEM showed that the deposition of CurSLN differed according to the type of polymer used. TEM confirmed the integrity of liberated CurSLN from sponges. IR spectra showed an interaction between HPMC and poloxamer 407, which affected its behavior as a gelling agent. The obtained results provide an efficient approach for delivering solid lipid nanoparticles in a solid dosage form keeping the nanoparticle characters and integrity. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. THE ROLE OF LABORATORY EXAMINATION IN RED EYE SYNDROME

    Directory of Open Access Journals (Sweden)

    G. M. Chernakova

    2015-01-01

    Full Text Available Aim: To analyze etiological structure of inflammatory diseases of ocular surface. Materials and methods: The study included outpatients (n = 49 who referred to Ophthalmological office at the Herpetic center during the period 2012 to 2014. Complaints duration was 60.65 ± 12.28 days. All patients underwent routine ophthalmologic examination; tear and/or conjunctival scraping PCR for type 1 and 2 herpes simplex viruses, varicella-zoster virus, cytomegalovirus, Epstein-Barr virus, type 6 human herpesvirus, adenoviruses, enteroviruses, ureaplasma, chlamydia; conjunctival swab microbiology; eyelash microscopy for eyelash mites. Results: After the examination, all patients were divided into three groups: 1 with herpesvirus infections of the eye (n = 24, 2 with non-herpetic infection of the eye (enterovirus, adenovirus, bacteria, fungi, n = 18, 3 with ocular surface lesions due to non-infectious causes (n = 7. In three cases U. urealyticum was detected, doxycycline was administrated. Identification of pathogens in the groups 1 and 2 resulted in effective causal treatment with subsequent clinical recovery. Exclusion of infection in the third group allowed to avoid inappropriate antibacterial or antiviral therapy. Conclusion: In patients with chronic red eye syndrome of unknown etiology, laboratory examination of tear and/or conjunctival scraping is essential for differentiating between infectious and non-infectious disease.

  1. Synthesis and characterization of (68)Ga-labeled curcumin and curcuminoid complexes as potential radiotracers for imaging of cancer and Alzheimer's disease.

    Science.gov (United States)

    Asti, Mattia; Ferrari, Erika; Croci, Stefania; Atti, Giulia; Rubagotti, Sara; Iori, Michele; Capponi, Pier C; Zerbini, Alessandro; Saladini, Monica; Versari, Annibale

    2014-05-19

    Curcumin (CUR) and curcuminoids complexes labeled with fluorine-18 or technetium-99m have recently shown their potential as diagnostic tools for Alzheimer's disease. Gallium-68 is a positron-emitting, generator-produced radionuclide, and its properties can be exploited in situ in medical facilities without a cyclotron. Moreover, CUR showed a higher uptake in tumor cells compared to normal cells, suggesting potential diagnostic applications in this field. In spite of this, no studies using labeled CUR have been performed in this direction, so far. Herein, (68)Ga-labeled complexes with CUR and two curcuminoids, namely diacetyl-curcumin (DAC) and bis(dehydroxy)curcumin (bDHC), were synthesized and characterized by means of experimental and theoretical approaches. Moreover, a first evaluation of their affinity to synthetic β-amyloid fibrils and uptake by A549 lung cancer cells was performed to show the potential application of these new labeled curcuminoids in these diagnostic fields. The radiotracers were prepared by reacting (68)Ga(3+) obtained from a (68)Ge/(68)Ga generator with 1 mg/mL curcuminoids solutions. Reaction parameters (precursor amount, reaction temperature, and pH) were optimized to obtain high and reproducible radiochemical yield and purity. Stoichiometry and formation of the curcuminoid complexes were investigated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, NMR, ultraviolet-visible, and fluorescence spectroscopy on the equivalent (nat)Ga-curcuminoids (nat = natural) complexes, and their structure was computed by theoretical density functional theory calculations. The analyses evidenced that CUR, DAC, and bDHC were predominantly in the keto-enol form and attested to Ga(L)2(+) species formation. Identity of the (68)Ga(L)2(+) complexes was confirmed by coelution with the equivalent (nat)Ga(L)2(+) complexes in ultrahigh-performance liquid chromatography analyses.(68)Ga(CUR)2(+), (68)Ga(DAC)2(+), and (68)Ga(bDHC)2

  2. Molluscicidal activities of curcumin-nisin polylactic acid nanoparticle on Biomphalaria pfeifferi.

    Directory of Open Access Journals (Sweden)

    Michael E Omobhude

    2017-08-01

    Full Text Available Snail intermediate host control is a widely canvassed strategy for schistosomiasis control in endemic countries. While there have been increasing studies on the search for potent molluscicides in the past years, the use of nanoparticulate agents as molluscicides is yet to gain wide attention. The aim of this study was to assess the molluscicidal potential of curcumin-nisin poly lactic acid (PLA entrapped nanoparticle (CurNisNp against Biomphalaria pfeifferi, a snail intermediate host for Schistosoma mansoni.CurNisNp formulated by double emulsion method was tested against the young adults, < 1 week, 1-2-week old juveniles, 1 day (blastula and 7 day-old (hippo-stage egg masses of B. pfeifferi. Mortality in the different stages was determined after 96-h of exposure at varying concentrations (350, 175, 87.5, 43.75 and 21.88 ppm. The sub-lethal effects of CurNisNp on the hatchability of the 7-day-old egg masses and egg laying capacity of the young adult snails were determined. The CurNisNp diameter, polydispersity index (PDI, zeta potential and drug entrapment efficiency were 284.0 ± 17.9 nm, 0.166 ± 0.03, -16.6 ± 2.45 mV and 35.0% respectively. The < 1 week old juveniles and the 1-day-old egg stage (blastula of B. pfeifferi with LC50 277.9 ppm and 4279.5 ppm were the most susceptible and resistant stages to the drug respectively. CurNisNp was also observed to cause significant reductions (P<0.05 in egg hatchability and egg laying capacity with strong negative correlation between egg laying capacity and concentration (r = -0.928; P<0.05.This study showed that CurNisNp has molluscicidal activities on different developmental stages of B. pfeifferi. It is therefore recommended that the formulation be more optimised to give a nanoparticle with a narrow range monodispersed PDI for better drug distribution and eventual greater molluscicidal activities.

  3. Development of antiproliferative long-circulating liposomes co-encapsulating doxorubicin and curcumin, through the use of a quality-by-design approach

    Directory of Open Access Journals (Sweden)

    Tefas LR

    2017-05-01

    Full Text Available Lucia Ruxandra Tefas,1 Bianca Sylvester,1 Ioan Tomuta,1 Alina Sesarman,2,3 Emilia Licarete,2,3 Manuela Banciu,2,3 Alina Porfire1 1Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Hatieganu”, 2Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, 3Molecular Biology Centre, Institute for Interdisciplinary Research in Bio-Nano-Sciences, Babeş-Bolyai University, Cluj-Napoca, Romania Abstract: The aim of this work was to use the quality-by-design (QbD approach in the development of long-circulating liposomes co-loaded with curcumin (CUR and doxorubicin (DOX and to evaluate the cytotoxic potential of these liposomes in vitro using C26 murine colon carcinoma cell line. Based on a risk assessment, six parameters, namely the phospholipid, CUR and DOX concentrations, the phospholipid:cholesterol molar ratio, the temperature during the evaporation and hydration steps and the pH of the phosphate buffer, were identified as potential risk factors for the quality of the final product. The influence of these variables on the critical quality attributes of the co-loaded liposomal CUR and DOX was investigated: particle size, zeta potential, drug loading and entrapment efficiency. For this, a 26-2 factorial design was employed to establish a proper regression model and to generate the contour plots for the responses. The obtained data served to establish the design space for which different combinations of variables yielded liposomes with characteristics within predefined specifications. The validation of the model was carried out by preparing two liposomal formulations corresponding to the robust set point from within the design space and one outside the design space and calculating the percentage bias between the predicted and actual experimental results. The in vitro antiproliferative test showed that at higher CUR concentrations, the liposomes co

  4. Glyphosate-Resistant Parthenium hysterophorus in the Caribbean Islands: Non Target Site Resistance and Target Site Resistance in Relation to Resistance Levels.

    Directory of Open Access Journals (Sweden)

    Enzo Bracamonte

    2016-12-01

    Full Text Available Glyphosate has been the most intensely herbicide used worldwide for decades, and continues to be a single tool for controlling weeds in woody crops. However, the adoption of this herbicide in a wide range of culture systems has led to the emergence of resistant weeds. Glyphosate has been widely used primarily on citrus in the Caribbean area, but a study of resistance in the Caribbean islands of Cuba and the Dominican Republic has never been carried out. Unfortunately, Parthenium hysterophorus has developed glyphosate-resistance in both islands, independently. The resistance level and mechanisms of different P. hysterophorus accessions (three collected in Cuba (Cu-R and four collected in the Dominican Republic (Do-R have been studied under greenhouse and laboratory conditions. In in vivo assays (glyphosate dose causing 50% reduction in above-ground vegetative biomass and survival, the resistance factor levels showed susceptible accessions (Cu-S≥Do-S, low-resistance accessions (Cu-R3Do-R2>Cu-R2>Do-R3>Do-R4>Cu-R3>>Cu-S≥Do-S. Glyphosate was degraded to aminomethylphosphonic acid, glyoxylate and sarcosine by >88% in resistant accessions except in Cu-R3 and Do-R4 resistant accessions (51.12 and 44.21, respectively, whereas a little glyphosate (<9.32% was degraded in both susceptible accessions at 96 h after treatment. There were significant differences between P. hysterophorus accessions in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS activity enzyme with and without different glyphosate rates. The R accessions showed values of between 0.026 and 0.21 µmol µg-1 TSP protein min-1 basal EPSPS activity values with respect to the S (0.024 and 0.025 accessions. The same trend was found in the EPSPS enzyme activity treated with glyphosate, where a higher enzyme activity inhibition (glyphosate µM corresponded to greater resistance levels in P. hysterophorus accessions. One amino acid substitution was found at position 106 in EPSPS, consisting

  5. Curcumin protects against tartrazine-mediated oxidative stress and hepatotoxicity in male rats.

    Science.gov (United States)

    El-Desoky, G E; Abdel-Ghaffar, A; Al-Othman, Z A; Habila, M A; Al-Sheikh, Y A; Ghneim, H K; Giesy, J P; Aboul-Soud, M A M

    2017-02-01

    Synthetic dyes have been reported to exert detrimental effects on the health of humans. This study evaluated the effects of a diet containing tartrazine (Tz) on rats which included: i) biochemical parameters including hepatic enzymes, kidney functions and profiles of lipids; ii) markers of oxidative stress in cells by measuring concentrations of malondialdehyde (MDA) and glutathione (GSH); iii) activities of selected, key hepatic antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx); iv) pathologies of liver. Also, protective effects of three doses of curcumin (CUR), a natural food coloring agent, on these parameters in rats that had been co-exposed to Tz. Fifty Wistar male albino rats were randomly divided into five groups: Group I, control, where rats were fed a normal diet; Group II, rats were fed normal diets containing 7.5 mg Tz/kg diet, dry mass (dm); In Groups III, IV and V, rats were fed diets containing Tz plus 1.0, 2.0 or 4.0 g CUR/kg diet, dm, respectively. Whole blood was collected after 90 d of exposure, homogenates of liver were prepared and the above analyses were conducted. Exposure to Tz in the diet caused statistically significant (peffects on functions of liver and kidney and the profile of relative concentrations of lipids. CUR significantly (peffects on enzymatic and non-enzymatic antioxidant and indicators of oxidative stress about rats fed Tz (Group II) to values in control rats. However, co-administration of 1.0 g CUR with Tz (Group III) exhibited a negligible effect on those parameters. The results of this study suggest benefits of the use of CUR, as a promising natural food additive to counteract oxidative stress caused by dietary exposure to the synthetic dye Tz due to potent protective antioxidant activity. Blending some natural food additives, such as CUR with diets containing synthetic dyes, could moderate potential effects of these artificial dyes. Decreasing or removing toxins in

  6. The international biological program/human adaptability studies among the Skolt Sami in Finland (1966–1970

    Directory of Open Access Journals (Sweden)

    Henrik Forsius

    2012-04-01

    Full Text Available Background: The population is increasingly lighter pigmented moving in a northward direction in Europe until reaching the Arctic Circle, where the Samis (Lapps are clearly more pigmented. Methods: In 1966–1970, we investigated a total of 689 subjects in the villages of Sevettijärvi and Nellim, including persons with mixed Sami and Finnish heritage; of these, 487 (242 males, 245 females had both parents classified as Skolt Sami. For estimation of the colour of the iris and hair, international scales were used. For translucency of the iris, pigmentation of the fundus was estimated in 3 different shades. The length and type of eyelashes were classified into 3 categories. To our knowledge, a simultaneous study of the pigmentation of eyebrows, eyelashes and eye fundus at different ages has not previously been published. Results: The age differences of iris colour were highly significant. Iris colour in children varied markedly, and they generally had lighter colours than later in life. Age and sex effects on the translucency of irises were found. Male irises were more translucent. Fundus pigmentation was scanty in the youngest age groups, with full pigmentation being reached at 20 years. Among young individuals hair colour darkens with increasing age. Eyebrow colour was slightly lighter for both sexes in the youngest age groups that in older cohorts. Women had longer eyelashes than males. Conclusions: The main factor of the lighter skin is a higher ability to synthesize vitamin D, providing superior protection against rickets. The Skolt Samis are more pigmented than other Nordic people. In earlier times they had problems with rickets but our studies did not show any essential symptoms of rickets today. Visual acuity among Skolt Samis was good. They had lower prevalence of myopia compared to Finns. The stronger pigmentation of Skolt Samis is probably due to their origin from darker Eastern populations. Since our investigations were made, the Skolt

  7. Unilateral corneal leukoplakia without limbal involvement

    Directory of Open Access Journals (Sweden)

    Hirano K

    2015-05-01

    Full Text Available Koji Hirano,1 Mihoko Koide,2 Yoshikazu Mizoguchi,3 Yasuhiro Osakabe,4 Kaoru-Araki Sasaki5 1Department of Ophthalmology, Ban Buntane Hotokukai Hospital, School of Medicine, Fujita Health University, Nagoya, Japan; 2Koide Internal Medicine and Eye Clinic, Nagoya, Japan; 3Department of Pathology, Ban Buntane Hotokukai Hospital, School of Medicine, Fujita Health University, Nagoya, Japan; 4Department of Molecular Pathology, Tokyo Medical University, Tokyo, Japan; 5Department of Ophthalmology, Japan Health Care Organization, Hoshigaoka Medical Center, Hirakata, Japan Purpose: Leukoplakia is the term given to a white patch or plaque that is found mainly on the oral mucus membrane. It can occasionally be seen on the corneal surface. We report our clinical and histopathological findings in a case of unilateral corneal leukoplakia. Methods: A 26-year-old woman was referred to our hospital because of a white patch on her right cornea that continued to expand. She first noticed the white patch when she was 20 years old, and the white patch had expanded to cover the pupillary area affecting her vision. After plastic surgery on both eyelids for bilateral entropion to alleviate the pain caused by the eyelashes rubbing the cornea, the white corneal patch decreased in size. Because of this reduction, we performed surgery to remove the patch with microforceps under topical anesthesia. The plaque was removed easily and completely, and submitted for histopathological examination. Results: Histopathological examination showed that the specimen had characteristics of epidermis with a basal cell layer, spinous cell layer, granular cell layer, and horny layer with hyperkeratosis. She was diagnosed with leukoplakia of the corneal surface. The basic structure of the squamous cell layer was preserved, and there were no signs of metaplasia. Six months after the removal of the leukoplakia, no recurrence was seen and her corrected decimal visual acuity recovered to 1

  8. Trichotillomania: Bizzare Patern of Hair Loss at 11-Year-old Girl.

    Science.gov (United States)

    Zímová, Jana; Zímová, Pavlína

    2016-06-01

    negative along the edges of the alopecia. Mycological examination from the skin capillitium was negative. The trichoscopy and skin biopsy of the parietotemporal region of the capillitium (Figure 3) confirmed trichotillomania. Laboratory tests (blood count, iron, ferritin, transferrin, selenium, zinc, vitamin B12, folic acid, serology and hormones of thyroid gland) were negative. We referred the girl for ophthalmologic and psychological examination. Ophthalmologic examination proved that there was no need to add any more diopters. The psychological examination provided us with a picture in which she drew her family (Figure 4). The strongest authority in the family was the mother because she looked after the girls for most of the day. She was in the first place in the picture. The father had longer working hours and spent more time outside the home. He worked as a long vehicle driver. He was in the second place in the picture. There was sibling rivalry between the girls, but the parents did not notice this problem and preferred the older daughter. She was successful at school and was prettier (slim, higher, curly brown hair, without spectacles). Our 11-years-old patient noticed all these differences between them, but at her level of mental development was not able to cope with this problem. She wanted to be her sister's equal. The sister is drawn in the picture in the third place next to father, while the patient's own figure was drawn larger and slim even though she was obese. Notably, all three female figures had very nice long brown hair. It seemed that the mother and our patient had better quality of hair and more intense color than the sister in the drawing. The only hairless person in the picture was the father. The girl did not want to talk about her problems and feelings at home. Then it was confirmed that our patient was very sensitive, anxious, willful, and withdrawn. She was interested in her body and very perceptive of her physical appearance. From the

  9. Small regulatory RNAs control the multi-cellular adhesive lifestyle of Escherichia coli

    DEFF Research Database (Denmark)

    Jørgensen, Mikkel Girke; Nielsen, Jesper Sejrup; Boysen, Anders

    2012-01-01

    Small regulatory RNA molecules have recently been recognized as important regulatory elements of developmental processes in both eukaryotes and bacteria. We here describe a striking example in Escherichia coli that can switch between a single-cell motile lifestyle and a multi-cellular, sessile...... and adhesive state that enables biofilm formation on surfaces. For this, the bacterium needs to reprogramme its gene expression, and in many E. coli and Salmonella strains the lifestyle shift relies on control cascades that inhibit flagellar expression and activate the synthesis of curli, extracellular...... adhesive fibres important for co-aggregation of cells and adhesion to biotic and abiotic surfaces. By combining bioinformatics, genetic and biochemical analysis we identified three small RNAs that act by an antisense mechanism to downregulate translation of CsgD, the master regulator of curli synthesis...

  10. Andrographolide: A Novel Antimalarial Diterpene Lactone Compound from Andrographis paniculata and Its Interaction with Curcumin and Artesunate

    Directory of Open Access Journals (Sweden)

    Kirti Mishra

    2011-01-01

    Full Text Available Andrographolide (AND, the diterpene lactone compound, was purified by HPLC from the methanolic fraction of the plant Andrographis paniculata. The compound was found to have potent antiplasmodial activity when tested in isolation and in combination with curcumin and artesunate against the erythrocytic stages of Plasmodium falciparum in vitro and Plasmodium berghei ANKA in vivo. IC50s for artesunate (AS, andrographolide (AND, and curcumin (CUR were found to be 0.05, 9.1 and 17.4 μM, respectively. The compound (AND was found synergistic with curcumin (CUR and addictively interactive with artesunate (AS. In vivo, andrographolide-curcumin exhibited better antimalarial activity, not only by reducing parasitemia (29%, compared to the control (81%, but also by extending the life span by 2-3 folds. Being nontoxic to the in vivo system this agent can be used as template molecule for designing new derivatives with improved antimalarial properties.

  11. Curcumin Targeted, Polymalic Acid-Based MRI Contrast Agent for the Detection of Aβ Plaques in Alzheimer's Disease.

    Science.gov (United States)

    Patil, Rameshwar; Gangalum, Pallavi R; Wagner, Shawn; Portilla-Arias, Jose; Ding, Hui; Rekechenetskiy, Arthur; Konda, Bindu; Inoue, Satoshi; Black, Keith L; Ljubimova, Julia Y; Holler, Eggehard

    2015-09-01

    Currently, there is no gadolinium-based contrast agent available for conventional magnetic resonance imaging (MRI) detection of amyloidal beta (Aβ) plaques in Alzheimer's disease (AD). Its timely finding would be vital for patient survival and quality of life. Curcumin (CUR), a common Indian spice effectively binds to Aβ plaques which is a hallmark of AD. To address this binding, we have designed a novel nanoimaging agent (NIA) based on nature-derived poly(β-l-malic acid) (PMLA) containing covalently attached gadolinium-DOTA(Gd-DOTA) and nature-derived CUR. The all-in-one agent recognizes and selectively binds to Aβ plaques and is detected by MRI. It efficiently detected Aβ plaques in human and mouse samples by an ex vivo staining. The method can be useful in clinic for safe and noninvasive diagnosis of AD. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Molluscicidal activities of curcumin-nisin polylactic acid nanoparticle on Biomphalaria pfeifferi.

    Science.gov (United States)

    Omobhude, Michael E; Morenikeji, Olajumoke A; Oyeyemi, Oyetunde T

    2017-08-01

    Snail intermediate host control is a widely canvassed strategy for schistosomiasis control in endemic countries. While there have been increasing studies on the search for potent molluscicides in the past years, the use of nanoparticulate agents as molluscicides is yet to gain wide attention. The aim of this study was to assess the molluscicidal potential of curcumin-nisin poly lactic acid (PLA) entrapped nanoparticle (CurNisNp) against Biomphalaria pfeifferi, a snail intermediate host for Schistosoma mansoni. CurNisNp formulated by double emulsion method was tested against the young adults, molluscicidal activities on different developmental stages of B. pfeifferi. It is therefore recommended that the formulation be more optimised to give a nanoparticle with a narrow range monodispersed PDI for better drug distribution and eventual greater molluscicidal activities.

  13. New Solutions to Line-Driven Winds of Hot Massive Stars

    Science.gov (United States)

    Gormaz-Matamala, Alex C.; Curé, Michel; Cidale, Lydia; Venero, Roberto

    2017-11-01

    In the frame of radiation driven wind theory (Castor et al.1975), we present self-consistent hydrodynamical solutions to the line-force parameters (k, α, δ) under LTE conditions. Hydrodynamic models are provided by HydWind (Curé 2004). We evaluate these results with those ones previously found in literature, focusing in different regions of the optical depth to be used to perform the calculations. The values for mass-loss rate and terminal velocity obtained from our calculations are also presented. We also examine the line-force parameters for the case when large changes in ionization throughout the wind occurs (δ-slow solutions, Curé et al.2011).

  14. Tomás de Aquino, Anselmo de Canterbury e a crítica da razão teológica = Aquinas and Anselm: a critique of theological reason

    Directory of Open Access Journals (Sweden)

    Jean Lauand

    2010-01-01

    Full Text Available Em 2009 se comemorou o 9º. centenário da morte de S. Anselmo (1033-1109. O artigo examina sua proposta de Teologia como ciência em contraste com a de Tomás de Aquino (1125-1274: as “rationes necessariae” anselmianas - no caso da Teologia da Redenção no "Cur Deus Homo?" - são confrontadas com as do Aquinate, contextualizadas pelo quadro geral do pensamento de Tomás.Nine hundred years after the death of Anselm of Canterbury, this article examines his idea of Theology as a science in contrast with Aquinas’s analysis of the same subject: the “rationes necessariae” ofAnselm – in Theology of Redemption in his work “Cur Deus Homo?” – are compared with the reasons of Thomas Aquinas, who emphasizes the liberty of God.

  15. Preparation, characterization and anti-colitis activity of curcumin-asafoetida complex encapsulated in turmeric nanofiber.

    Science.gov (United States)

    Gopi, Sreeraj; Amalraj, Augustine; Jude, Shintu; Varma, Karthik; Sreeraj, T R; Haponiuk, Józef T; Thomas, Sabu

    2017-12-01

    Ulcerative colitis (UC) is a main form of inflammatory bowel disease (IBD). Asafoetida (ASF) and turmeric have traditionally been used for the treatment of various inflammatory diseases, including UC, because ASF is rich in sulfur compounds and turmeric contains curcumin (CUR). Turmeric nanofiber (TNF), the modified cell wall component of turmeric is considered to play important role in the human diet, health and can be used as a carrier agent to encapsulate bioactive components. A novel gut health product (GHP) was formulated by encapsulation of ASF and CUR complex onto TNF. The GHP was characterized by UPLC, GC-MS, FTIR, XRD, SEM with EDS and DSC studies. GHP was evaluated for anti-colitis activity in a rat model of 5% dextran sulfate sodium (DSS) induced UC. Treatment with GHP significantly attenuated the disease activity index, colitis score, histopathological changes and myeloperoxidase activity. GHP has significant protective effects against DSS induced colitis. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. A Smart Assistant for Shooting Virtual Cinematography with Motion-Tracked Cameras

    OpenAIRE

    Lino , Christophe; Christie , Marc; Ranon , Roberto; Bares , William

    2011-01-01

    International audience; This demonstration shows how an automated assistant encoded with knowledge of cinematography practice can off er suggested viewpoints to a fi lmmaker operating a hand-held motion-tracked virtual camera device. Our system, called Director's Lens, uses an intelligent cinematography engine to compute, at the request of the lmmaker, a set of suitable camera placements for starting a shot that represent semantically and cinematically distinct choices for visualizing the cur...

  17. The Representation of Workingwomen in Spanish Television Fiction

    OpenAIRE

    Lacalle, Charo; Gómez, Beatriz (UNIR)

    2016-01-01

    During the sixties and seventies the limited presence of women in the public sphere was reflected in the restricted repertoire of roles played by female characters in television fiction (mainly those of mothers and wives). The strengthening of the feminist movement in the following decades increased and diversified the portrayals of women in the workplace, and further encouraged academic research on the social construction of working women. Despite the relevance of female professionals in cur...

  18. Written corrective feedback in secondary education: learners' and teachers' preferences and perceptions

    OpenAIRE

    Martínez Buffa, Ignacio

    2016-01-01

    Treball Final de Màster Universitari en Ensenyament i Adquisició de la Llengua Anglesa en Contextos Multilingües (Pla de 2013). Codi: SAY031. Curs: 2015/2016 The role of corrective feedback (CF, henceforth) in second language acquisition (SLA, henceforth), more specifically written corrective feedback (WCF, henceforth), has been highly studied in the last couple of decades (Truscott, 1996; 1999; Ferris, 1999; Ashwell, 2000; Chandler, 2003; Russell & Spada, 2006; Sheen, 2010; 2...

  19. Publicidad nativa em Instangram: el fenómeno de los influencers y la moda

    OpenAIRE

    García Mestre, Aitana

    2017-01-01

    Treball final de Grau en Publicitat i Relacions Públiques. Codi: PU0932. Curs acadèmic: 2016/2017 Traditional advertising has led companies to search for a market niche inside the digital environment and, in particular, inside social networks, where they can develop their activity. The new environment involves the appearance of a more demanding consumer that would like to take part on the content production, so it is required a figure that acts as an opinion leader; this is the...

  20. The management of support groups for patients with chronic diseases

    OpenAIRE

    2010-01-01

    M.Cur. The role players participating within the support groups for patients with chronic diseases in Primary Health care services are facing a difficult task of managing support groups effectively. They are confronted by managerial challenges involved in the managing of support groups for patients with chronic diseases, such as careful planning, organising, leading and controlling the support groups. This is evidently confirmed by the disintegration of support groups and patients openly a...

  1. Kernel-based manifold visualization of GPCR sequences

    OpenAIRE

    Cárdenas Domíınguez, Martha Ivón

    2011-01-01

    G-Protein Coupled Receptors (GPCRs) are key players in cell- cell communication. They transduce a wide range of extracellular signals such as light, odors, hormones or neurotransmitters into ap- propriated cellular responses. These receptors regulate many cell functions and are encoded by the largest gene family in mammalian genomes, representing more than 3% of the human genes. GPCRs are the estimated target of approximately half of the medicines cur- rently in clinical ...

  2. Instabilities of Damage and Surface Degradation Mechanisms in Brittle Material Structural Systems

    Science.gov (United States)

    1992-03-15

    radiography and diffraction have been developed as non-destructive techniques (NDT), to assess the integrity of structures, machines and other systems...Most of the methods cur- rently employed rely on the inspection of the determinant of the tangent stiffness matrix, and employ simple bisection ...offer very attractive alternatives to radiation. Each NDT has its own advantages and limitations. A major problems of many techniques is that the

  3. Environmental Impact Statement. Disposal and Reuse of Chanute Air Force Base, Illinois

    Science.gov (United States)

    1991-07-01

    aster (Aster sp.), goldenrod (Solidago sp.), prairie violet (Viola p6datffida), and white wild Indigo (Baptisia leucantha). Land surrounding Chanute AFB...a card? 2 error was created because ttiei did not proparl) MAYOR PQDA1;hOGII May I make one more S research cur State kid Fare from the Stat, of...to operate within th Village forms. The Village contises this thy will hecoime Nh owner Kid operiato fte pubic Pt" Iteof SN rlectric systmss 2012

  4. Lliçó inaugural: "L'aroma de la innovació"

    OpenAIRE

    Cornellà i Valls, Pere

    2005-01-01

    Llicó inaugural del curs acadèmic 2005-06 de les universitats catalanes, a càrrec del Sr.Pere Cornellà. A partir de la formulació d'una premissa desenvolupa tot un discurs al voltant del cafè, la seva història, la manera d'entendre el cafè lligat amb la innovació, la universitat i l'empresa

  5. Evaluación e intervención psicológica en pacientes con lumbalgia crónica atendidos en el hospital Arnau de Vilanova (Valencia)

    OpenAIRE

    Gregori Coronado, Jessica

    2014-01-01

    Treball Final de Grau en Psicologia. Codi: PS1048. Curs acadèmic 2013-2014 Chronic low back pain, the most common chronic pain syndrome in Europe, consists of a persistent pain in the muscles located in the lower back that exceeds three months. Psychological interventions are effective, alone or as part of a multidisciplinary intervention, for chronic low back pain both of known and unknown etiology. This research tested preliminary a cognitive behavioral therapy program to determine it...

  6. Use of Welfare Technology in Elderly Care

    OpenAIRE

    Woll, Anita

    2017-01-01

    This thesis is an interpretive qualitative research study exploring the use of welfare technology in elderly care. In Norway, health care and social services for elderly people are important parts of the welfare policy. A growing elderly population is putting a strain on the society’s welfare system. Scarce welfare resources restrict human care services for our future generations. Alternative housings and the use of welfare technology are effective techniques that can be used to transform cur...

  7. The (R) evolution and future of banking system and commerce

    OpenAIRE

    Neusa Pinto, Daniel Andrés

    2017-01-01

    Treball Final de Grau. Grau en Finances i Comptabilitat. Codi: FC1049. Curs acadèmic 2016-2017 The aim of this paper is providing information giving an overview about a new age in the financial field. Financial Technology (Fintech) industry is growing faster than other industries and it is changing the way we know the financial markets, customer’s behavior, banking system, payments and, in general, the interaction between financial clients and the financial system. Banks are on...

  8. Oceanic circulation models help to predict global biogeography of pelagic yellow-bellied sea snake

    OpenAIRE

    Brischoux, François; Cotté, Cédric; Lillywhite, Harvey B.; Bailleul, Frédéric; Lalire, Maxime; Gaspar, Philippe

    2016-01-01

    It is well recognized that most marine vertebrates, and especially tetrapods, precisely orient and actively move in apparently homogeneous oceanic environments. Here, we investigate the presumptive role of oceanic currents in biogeographic patterns observed in a secondarily marine tetrapod, the yellow-bellied sea snake (Hydrophis [Pelamis] platurus). State-of-the-art world ocean circulation models show how H. platurus, the only pelagic species of sea snake, can potentially exploit oceanic cur...

  9. Interpreting physical flows in networks as a communication system

    Indian Academy of Sciences (India)

    1Universidad de la República, Instituto de Física de la Facultad de Ciencias, Iguá 4225, Montevideo,. 11400, Uruguay. 2University of Aberdeen, King's ... E-mail: nrubido@fisica.edu.uy. Abstract. Information theory concepts and .... s-t, F(st) is the flow vector containing the total cur- rent at each node (i.e., [F(st)]i = j f. (s, t).

  10. The core-curriculum as "Idealtypus " : life -centered education which has "Activities"in the center of a curriculum

    OpenAIRE

    金馬, 国晴

    2004-01-01

    In the field of education, an important question has been how to reconstruct and integrate a curriculum which is sectionalized by each subject. Some educators have introduced seikatsu, which means "living experiences" or "activities", into the curriculum. There are many theories, practices or movements of seikatsu kyoiku, or life-centered education. The Core-Curriculum, one type of New Education in the early post World War II period, will be discussed and redefined in this paper. The Core-Cur...

  11. Historia clínica y examen radiográfico a propósito de un caso de "mielomas multiples"

    Directory of Open Access Journals (Sweden)

    Guillermo de Angulo

    1939-12-01

    Antecedentes patológicos: Además de las enfermedades de la infancia: sarampión, tos ferina y varicela, padeció de una pulmonía a los 23 años que, le curó después de ocho días sin dejarle secuelas. A los 32 años tuvo varios accidentes disentéricos.

  12. Aprendizaje por Proyectos: Una Aproximación Docente al Diseño Digital Basado en VHDL

    OpenAIRE

    Rosado Muñoz, Alfredo; Bataller Mompean, Manuel; Guerrero Martínez, Juan Francisco

    2008-01-01

    Abstract-- Digital design based on hardware description languages is difficult for students, especially when the course covers from basics to advanced design systems and hardware implementation topics. This paper describes the proposal of a course where students have basic knowledge in digital design but null knowledge in hardware description languages as VHDL and FPGA (Field Programmable Gate Array) devices. Using Project Based Learning (PBL), this proposal allows increasing the learning cur...

  13. Military Handbook: Management and Design Guidance Electromagnetic Radiation Hardness for Air Launched Ordnance Systems

    Science.gov (United States)

    1981-01-15

    system is attacted to the delivery aircraft until it Impacto a target, it is exposed to electromagnetic radiation from emitters aboard the delivery...homogeneous, isotropic, ambient medium may be a lossy dielectric. Antenna computations include cur- rent distribution, input impedance, radiation...permissible ambient interference level in the system, and when determining the expected signal-to-inter- ference ratio of the signal transmission circuits

  14. Basic Processes of Plasma Propulsion

    Science.gov (United States)

    1991-04-01

    compensation (means) and orbit maneuvering. With the advent of onboard nuclear power plants of 100kW electric and more high power arcjets and even MPD...advanced thrusters are the high power arcjets while MPD thrusters are cur- rently investigated up into the several hundred kilowatt range in steady state...Propuls. Conf. Orlando, Fl. July 1990 [4] T. Yamada, Y. Shimizu, K. Toki and K. Kuriki, " Thermal Analysis and Thrust Performance of a Low Power

  15. Geophysical Survey of McMurdo Ice Shelf to Determine Infrastructure Stability and for Future Planning

    Science.gov (United States)

    2017-01-01

    Laboratory (CRREL) 72 Lyme Road Hanover, NH 03755-1290 Joel Wilner Department of Geology McCardell Bicentennial Hall 276 Bicentennial Way Middlebury...EPOLAR) EP- ANT -15-36, “Geophysical Survey of McMurdo Ice Shelf to Determine Cur- rent Infrastructure Stability and for Future Planning” ERDC/CRREL TR...threaten research in Antarctica. Researchers at the U.S. Army Cold Regions Research and Engineering Laboratory collected approximately 1300 km of

  16. What Does a Swiss Franc Mortgage Cost? The Tale of Polish Trust for Foreign Currency Denominated Mortgages: Implications for Well-Being and Health

    OpenAIRE

    Bia?owolski, Piotr; W?ziak-Bia?owolska, Dorota

    2016-01-01

    It is commonly agreed that excessive household financial debts are detrimental to psychological and physical health. Research also demonstrates that housing instability, mortgage indebtedness and mortgage foreclosure negatively influence subjective well-being. In Poland at the beginning of 2015, homeowners with Swiss franc denominated mortgages suffered from an abrupt swing in the Swiss franc/Polish zloty (CHF/PLN) exchange rate, which resulted in the considerable increase of the local cur...

  17. Aplikace moderních prvků v klasickém projektovém řízení

    OpenAIRE

    Országh, Martin

    2013-01-01

    This thesis deals with project management and with different ways in which can be cur-rently approached. Beside the conventional, classical approach based on processes, modern approaches based on the theory of constraints and agile principles are discussed. Based on analysis, the main objective of the thesis is firstly to find weaknesses in the classical project management and strong key elements of modern approaches and after that to make suggestions to enhance the classical approach with th...

  18. Historický vývoj pohybové skladby a její zařazení v soutěži Teamgym

    OpenAIRE

    Maroušková, Zuzana

    2014-01-01

    Title: The historical development of movement (gymnastics) composition and its inclusion in Teamgym competition. Objectives: To describe historical development, current inclusion in Teamgym and present contain and choreographic trends. Methods: In my thesis I mostly used analytic and synthetic method based on consolidation of data from written and video sources and their organization in content of my thesis and context of time period. Results: Description of the historical development and cur...

  19. Prestasiebeoordeling van akademiese personeel aan 'n verplegingskollege

    OpenAIRE

    2014-01-01

    M.Cur. Performance assessment of academic staff (tutor) at a nursing college is an essential component in the establishment of a well-prepared teacher corps which contributes to quality nursing education. There is widespread 'dissatisfaction with the quality of performance assessment of academic staff (tutor). Factors contributing to the ineffectiveness of performance achievement are linked to the performance assessment system itself, on the one hand, and to the person who performs the ass...

  20. Alice KV On the limit-classifications of even and odd-order formally ...

    Indian Academy of Sciences (India)

    R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

    Inverse solutions for a second-grade fluid for porous medium channel and Hall cur- rent effects. 79. Nadkarni M G see Kłopotowski A. 181. Navada K Gowri. Some remarks on good sets. 389. Nitsure Nitin. Representability of Hom implies flatness. 7. ¨Oztürk Sermin see Yıldırım Huseyin. 375. Padmanabhan A see Alice K V.

  1. National Needs for Critically Evaluated Physical and Chemical Data.

    Science.gov (United States)

    1978-01-01

    Scientific Laboratory Orson L. Anderson University of California, Los Angeles Fay Ajzenberg-Selove University of Pennsylvania Edward P. Bartkus E. I. du...the ICSU Committee on Data for Science and Technology (CODATA), play an active role. Cur- rent U.S. data programs appear to be well coordinated with...systems. In contrast to the above example dealing with an urgent national need and having a well -defined path from science to application, the importance of

  2. SIMULATION STUDIES FOR UPQC

    OpenAIRE

    Dr. PRASAD M. JOSHI; Mukul Kumar

    2016-01-01

    Nowadays, widespread use of power electronics based load in house-hold and industry have increased the importance and application of power Quality studies. Power electronic devices having property of non-linearity draw harmonic and reactive power from the source. Unified Power Quality conditioner (UPQC) is the device that mitigates the harmonics and improves the quality of power. This paper presents the working principle of UOQC based on instantaneous p-q theory which compensates the load cur...

  3. Navy Tactical Applications Guide. Volume 2. Environmental Phenomena and Effects

    Science.gov (United States)

    1979-01-01

    Mexico and surrounding land areas. Numerous bright spots, produced by city lights, appear over the land areas. Some of the larger metro - politan areas...10 0250 CLR 7 0249 CUR 10 0350 CLR 7 0350 CLR 10 0450 CLR 6 11 0449 CIR 85 0 0 250- SCT 4 H 0549 CLR 8 0650 250- SCT 4 H 0648 CLR 8 2C-5 Case 3 Fog, Haze

  4. Modulation of anxiety behavior by intranasally administered vaccinia virus complement control protein and curcumin in a mouse model of Alzheimer's disease.

    Science.gov (United States)

    Kulkarni, A P; Govender, D A; Kotwal, G J; Kellaway, L A

    2011-02-01

    Widespread neuroinflammation in the central nervous system (CNS) of Alzheimer's disease (AD) patients, involving pro-inflammatory mediators such as complement components, might be responsible for AD associated behavioral symptoms such as anxiety. Vaccinia virus complement control protein (VCP) and curcumin (Cur) are the bioactive compounds of natural origin shown to inhibit the in-vitro complement activation. In order to develop complement regulatory compounds which could be delivered to the CNS by a non-invasive route, VCP, its truncated version (tVCP), and Cur were administered to Wistar rats intranasally. The distribution of these compounds in cerebrospinal fluid (CSF) was studied using an enzyme linked immunosorbent assay (ELISA), using VCP and tVCP as antigens and a modified fluorimetric method (Cur). VCP and tVCP were also detected in the olfactory lobes of the rat brain using immunohistochemical analysis. These compounds were then compared for their ability to attenuate the anxiety levels in APPswePS1δE9 mice using an elevated plus maze (EPM) apparatus. VCP treatment significantly improved the exploratory behavior and reduced the anxiety behavior in APPswePS1δE9 mice. tVCP however showed an opposite effect to VCP, whereas Cur showed no effect on the anxiety behavior of these mice. When these mice were subsequently tested for their cognitive performance in the Morris water maze (MWM), they showed tendencies to collide with the periphery of the walls of MWM. This unusual activity was termed "kissperi" behavior. This newly defined index of anxiety was comparable to the anxiety profile of the VCP and tVCP treated groups on EPM. VCP can thus be delivered to the CNS effectively via intranasal route of administration to attenuate anxiety associated with AD.

  5. Química general II

    OpenAIRE

    Olba Torrent, Amparo

    2018-01-01

    El document forma part dels materials docents programats mitjançant l'ajut del Servei de Política Lingüística de la Universitat de València Temes de l'assignatura: Química general II del primer curs del Grau de Química Topics of the course: General Chemistry II in the first year of the Degree in Chemistry

  6. Gamification Quest: Rhythm. Music as a game mechanic

    OpenAIRE

    Granell Díaz, Marina

    2017-01-01

    Treball Final de Grau en Disseny i Desenvolupament de Videojocs. Codi: VJ1241. Curs acadèmic: 2016/2017 This document constitutes the Technical Report for the project Gamification Quest: Rhythm, music as a game mechanic for the Videogame Design and Development bachelor degree. The project consists on the design and implementation of rhythm game mechanics integrated in a gamification environment applied to education. The video game will be implemented on the game engine Unity (10), ...

  7. Journal of Special Operations Medicine Volume 1, Edition 2

    Science.gov (United States)

    2001-01-01

    from hives to anaphy- laxis. No fatalities have been reported. Two cases of Guillian - Barre (ascending paralysis) syndrome have oc- curred following...developed decreased right finger movement. Nerve conduction studies were consistent with carpal tunnel syndrome . On October 19, while travelling in...any hematocrit under 30% was sufficient indica- tion for a couple units of blood. Then gay-related im- mune deficiency syndrome (GRIDS), later to be

  8. Strategies for the assessment of critical thinking in a four year nursing programme

    OpenAIRE

    2014-01-01

    D.Cur. (Professional Nursing Science ) The nursing practice needs critical thinkers because of diverse and rapidly changing developments and desired outcomes in health care. These developments are based on health and informational changes, organisational and cultural change sin nursing education, changes in the scope o fpractice, and increasing patient acuity and the movement of patient care from acute care facility to community selling have increased demands for nurses to think critically...

  9. The emotional intelligence of professional nurses commencing critical care nursing in private hospitals in Gauteng

    OpenAIRE

    Nagel, Yvette Juanita

    2015-01-01

    M.Cur. (Nursing Science) The primary objective of this study was to determine the emotional intelligence (EI) of, and make recommendations to facilitate an improvement in the EI of professional nurses commencing work in critical care units in private hospitals in Gauteng, South Africa. The quality of nursing care directly affects patient outcomes such as morbidity, mortality, adverse events as well as the total cost of care. This places the nurse central in good, comprehensive health care,...

  10. Polyelectrolyte Complex Nanoparticles from Chitosan and Acylated Rapeseed Cruciferin Protein for Curcumin Delivery.

    Science.gov (United States)

    Wang, Fengzhang; Yang, Yijie; Ju, Xingrong; Udenigwe, Chibuike C; He, Rong

    2018-03-21

    Curcumin is a polyphenol that exhibits several biological activities, but its low aqueous solubility results in low bioavailability. To improve curcumin bioavailability, this study has focused on developing a polyelectrolyte complexation method to form layer-by-layer assembled nanoparticles, for curcumin delivery, with positively charged chitosan (CS) and negatively charged acylated cruciferin (ACRU), a rapeseed globulin. Nanoparticles (NPs) were prepared from ACRU and CS (2:1) at pH 5.7. Three samples with weight of 5%, 10%, and 15% of curcumin, respectively, in ACRU/CS carrier were prepared. To verify the stability of the NPs, encapsulation efficiency and size of the 5% Cur-ACRU/CS NPs were determined at intervals of 5 days in a one month period. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction, and differential scanning calorimetry confirmed the electrostatic interaction and hydrogen bond formation between the carrier and core. The result showed that hollow ACRU/CS nanocapsules (ACRU/CS NPs) and curcumin-loaded ACRU/CS nanoparticles (Cur-ACRU/CS NPs) were homogenized spherical with average sizes of 200-450 nm and zeta potential of +15 mV. Encapsulation and loading efficiencies were 72% and 5.4%, respectively. In vitro release study using simulated gastro (SGF) and intestinal fluids (SIF) showed controlled release of curcumin in 6 h of exposure. Additionally, the Cur-ACRU/CS NPs are nontoxic to cultured Caco-2 cells, and the permeability assay indicated that Cur-ACRU/CS NPs had improved permeability efficiency of free curcumin through the Caco-2 cell monolayer. The findings suggest that ACRU/CS NPs can be used for encapsulation and delivery of curcumin in functional foods.

  11. Bevordering van kommunikasie en samewerking tussen oorplantingskoördineerders en intensiewesorgverpleegkundiges tydens die orgaanskenkingsproses

    OpenAIRE

    2012-01-01

    M.Cur. Various factors influence the process of organ donation. As a result there is a shortage of organs for transplant. Poor communication and cooperation between transplant coordinators and intensive care nursing staff can lead to the non-referral of potential organ donors, which is detrimental to the process. The purpose of the research is to ascertain the attitude and knowledge of the transplant coordinators and intensive care nursing staff who work in intensive care units during orga...

  12. Blunting the Spear: Why Good People Get Out

    Science.gov (United States)

    2015-03-01

    the current monthly ACIP for eligible officers. RIGHTSIZING AND REQUIREMENTS EXPLAINED 26 Years of Aviation Service M o n th ly M o n et ar y P ay...fighter pilots reach burnout . Recapitalization of human assets must oc- cur at a rate coincident with our material assets, as exemplified by the use of...been continuously engaged in war. The number of pilots pulled to operate a nonprimary weap- ons system was unable to meet the growing demand without

  13. Acondicionamiento de un local comercial para un estudio de arquitectura e ingeniería

    OpenAIRE

    Cruzado Abad, Enrique

    2016-01-01

    Treball final de Grau en Arquitectura Tècnica. Codi: ED0945. Curs acadèmic 2015-2016 Mediante el presente proyecto lo que se pretende es realizar un cambio de uso de un local a un estudio de arquitectura e ingeniería, con el correspondiente cumplimiento de la normativa destinada a este uso, además de conseguir el otorgamiento de las correspondientes licencias para la apertura de este estudio.

  14. A Statistical Model of Radar Bird Clutter at the DEW Line

    Science.gov (United States)

    1991-05-01

    Iswbuctions. serching .rxasg data ecure.. gathering and mcaintaining the data needed, and conmpleting and reviewing the collecion of information. Send...species of birds would have a particular RCS is determined. Also, probability cur-ves are derived that predict how many flocks out of those observed...probability curve, we can use the equation to portray the number of birds per day. If the number of birds per day is fl(x), and x is the day of travel

  15. Operation IVY: 1952

    Science.gov (United States)

    1982-12-01

    per cubic centimeter). A curie is a measure of radio- activity (3.7 x 10 disintegrations per second). Thus, for example, the max- imum beta or gamma...the decision to allow the 20-R exposure could be a conb-4Tand "* The abbreviation "c’ was commonly used in 1952 to represent " curie ;" the cur- rently...Jedrol - Jieroru Bogombogo BELLE - Bokombako Bogon IRENE - Boken Bokaidrik HELEN - Bokaidrikdrik - Bogairikk - Bogeirik Bokaidrikdrik HELEN - Bogairikk

  16. Coca-Cola’s case

    OpenAIRE

    Fabregat García, Noelia

    2015-01-01

    Treball final de Grau en Finances i Comptabilitat. Codi: FC1049. Curs acadèmic 2014-2015 Coca-Cola’s case is a research paper based on cocacola bottling companies in Spain. Its aim is to analyse its financial statements and the conclusions about its corresponding profitability and liquidity indicators to finally reach the conclusion that this large reorganizations and the labour force adjustment plan suffered were not really argued and the alleged causes were not certa...

  17. Operational Models of Infrastructure Resilience

    Science.gov (United States)

    2015-01-01

    Wiemer S. A stochastic forecast of California earth - quakes based on fault slip and smoothed seismicity. Bulletin of the Seismological Society of America...mean time between failures). For so- called rare events there is ongoing debate about how to model the frequencies with which disruptions oc- cur (e.g...in a simple priority list of importance; however, the frequency with which a link appears in attack or defense solutions provides an indication of

  18. Current Issues in Maritime Health

    DEFF Research Database (Denmark)

    Wagtmann, Maria Anne

    2008-01-01

     In the early part of 2008, Maria Anne Wagtmann had the opportunity to interview the former president of the International Maritime Health Association, Dr. Tim Carter, in London about a number of current maritime health issues. In this interview, Dr. Tim Carter, who is cur­rently employed as the ...... as the Chief Medical Advisor for the British government's Department for Transport, gives his personal - and thus non-official - opinions on these issues....

  19. Curcumin-carboxymethyl chitosan (CNC) conjugate and CNC/LHR mixed polymeric micelles as new approaches to improve the oral absorption of P-gp substrate drugs.

    Science.gov (United States)

    Ni, Jiang; Tian, Fengchun; Dahmani, Fatima Zohra; Yang, Hui; Yue, Deren; He, Shuwang; Zhou, Jianping; Yao, Jing

    2016-11-01

    The low oral bioavailability of numerous drugs has been mostly attributed to the significant effect of P-gp-mediated efflux on intestinal drug transport. Herein, we developed mixed polymeric micelles (MPMs) comprised of curcumin-carboxymethyl chitosan (CNC) conjugate, as a potential inhibitor of P-gp-mediated efflux and gastrointestinal absorption enhancer, and low-molecular-weight heparin-all-trans-retinoid acid (LHR) conjugate, as loading material, with the aim to improve the oral absorption of P-gp substrate drugs. CNC conjugate was synthesized by chemical bonding of curcumin (Cur) and carboxymethyl chitosan (CMCS) taking advantage of the inhibition of intestinal P-gp-mediated secretion by Cur and the intestinal absorption enhancement by CMCS. The chemical structure of CNC conjugate was characterized by 1 H NMR with a degree of substitution of Cur of 4.52-10.20%. More importantly, CNC conjugate markedly improved the stability of Cur in physiological pH. Cyclosporine A-loaded CNC/LHR MPMs (CsA-CNC/LHR MPMs) were prepared by dialysis method, with high drug loading 25.45% and nanoscaled particle size (∼200 nm). In situ single-pass perfusion studies in rats showed that both CsA + CNC mixture and CsA-CNC/LHR MPMs achieved significantly higher K a and P eff than CsA suspension in the duodenum and jejunum segments (p CNC mixture and CsA-CNC/LHR MPMs significantly increased the oral bioavailability of CsA as compared to CsA suspension. These results suggest that CNC conjugate might be considered as a promising gastrointestinal absorption enhancer, while CNC/LHR MPMs had the potential to improve the oral absorption of P-gp substrate drugs.

  20. An examination of different etiological pathways to alcohol use and misuse in adolescence

    OpenAIRE

    López Fernández, Francisco Javier

    2016-01-01

    Treball Final de Màster Universitari en Investigació en Cervell i Conducta. Codi: SBM024. Curs: 2015/2016 Alcohol abuse, especially among adolescents, causes important health, economic and social problems. Different theoretical pathways have been suggested for the etiology of alcohol use and abuse. The aim of our study was to describe some of these different etiological pathways in adolescents. We explored the importance and specific role of personality, cognitive variables (m...

  1. Marine Forage Fishes in Puget Sound

    Science.gov (United States)

    2007-03-01

    spawning is known to oc- cur in both southern Puget Sound and the Strait of Georgia ( Whatcom County) during the summer months, suggesting resident...commonly ends in nearly all areas by early April (Figure 3). The Cherry Point herring stock of western Whatcom County is un- usual among Washington...substrate (Figure 7). In certain parts of the Puget Sound Basin, especially western Whatcom County, the intertidal and shallow subtidal marine algal turf

  2. In-Use and Emerging Disruptive Technology Trends

    Science.gov (United States)

    2015-03-31

    Windows Server and the Azure cloud service. (Zander 2014) Although this cur- rently limits the utility of this particular program, it does not limit...to container technology in the market place. Major compa- nies supporting Docker include Microsoft ( Azure ), Google (Com- pute Engine), Red Hat...14, 2015) Zander, Jason. “New Windows Server containers and Azure support for Docker.” Octo- ber 15, 2014. azure.microsoft.com/blog/2014/10/15/new

  3. Lightning Technology.

    Science.gov (United States)

    1980-04-01

    thunder . The thunder signals were recorded on a second FM magnetic tape channel. In this manner, the time of occurrence, the nature of the lightning ...from the Kiva to the lightning cur- rent element of interest is based on the assumption that the time from flash to thunder multiplied by the speed of...some corona-like discharges before the main lightning event. The acoustically determined (per section 4) thunder sources are displayed in figure 5.4

  4. Endoscopic Treatment for Early Gastric Cancer

    OpenAIRE

    Kim, Sang Gyun

    2011-01-01

    Endoscopic resection has been accepted as a curative modality for early gastric cancer (EGC). Since conventional endoscopic mucosal resection (EMR) has been introduced, many improvements in endoscopic accessories and techniques have been achieved. Recently, endoscopic submucosal dissection (ESD) using various electrosurgical knives has been performed for complete resection of EGC and enables complete resection of EGC, which is difficult to completely resect in the era of conventional EMR. Cur...

  5. Electrochemical polymer electrolyte membranes

    CERN Document Server

    Fang, Jianhua; Wilkinson, David P

    2015-01-01

    Electrochemical Polymer Electrolyte Membranes covers PEMs from fundamentals to applications, describing their structure, properties, characterization, synthesis, and use in electrochemical energy storage and solar energy conversion technologies. Featuring chapters authored by leading experts from academia and industry, this authoritative text: Discusses cutting-edge methodologies in PEM material selection and fabricationPoints out important challenges in developing PEMs and recommends mitigation strategies to improve PEM performanceAnalyzes the cur

  6. Bio systematics of two species of the genus globular ia L. in Jordan and Tuns ia (Research Note)

    International Nuclear Information System (INIS)

    Oran, A. S.; Kochkar, N.; Raies, A.

    1999-01-01

    The status of the genus Globular ia L. in Jordan and Tunisia is presented. Bio systematic data related to morphology, habitats, geographical distribution, pollen and seed morphology as revealed by both light and scanning electron microscopy were studied based on material collected from both countries. Based on the collected bio systematic information on, this study has confirmed that only one species Globular ia arabica ac curs in Jordan and one species, G. alypum occurs in Tunisia. (authors). 12 refs., 1 table. 1 fig

  7. Devenir De l'azote de L'engrais enrichi au n applique au tournesol ...

    African Journals Online (AJOL)

    L'utilisation de I' azote de I' engrais par la culture de toumesol a ete appreciee par Ia determination du coefficient d'utilisation apparent (CUA) estime par Ia methode de difference et du coefficient d'utilisation reel (CUR) estime par Ia methode isotopique. 80 kg N ha-1 a 4.87% d'atomes en exces de 15N ont ete appliques au ...

  8. San Antonio Creek Restoration, Vandenberg Air Force Base, California

    Science.gov (United States)

    2008-06-27

    quarries located in Santa Margarita (San Luis Obispo County), and Corona (Riverside County). Currently, truck access to the Curly Road borrow pit is...10 inches in height spanning the vertical range of the pit, was removed from each hole. Soil color and texture were characterized from this sample...Soil color was determined by the comparison of moist samples to the color plates in the Munsell Soil Color Charts (2000). Texture was evaluated

  9. Environmental Assessment: San Antonio Creek Restoration at Vandenberg Air Force Base, California

    Science.gov (United States)

    2008-09-08

    Curly Road on north VAFB, and quarries located in Santa Margarita (San Luis Obispo County), and Corona (Riverside County). Currently, truck access...range of the pit, was removed from each hole. Soil color and texture were characterized from this sample. Soil color was determined by the comparison...of moist samples to the color plates in the Munsell Soil Color Charts (2000). Texture was evaluated by touch, following procedures adapted from

  10. Hyperspectral image representation and processing with binary partition trees

    OpenAIRE

    Valero Valbuena, Silvia

    2012-01-01

    Premi extraordinari doctorat curs 2011-2012, àmbit Enginyeria de les TIC The optimal exploitation of the information provided by hyperspectral images requires the development of advanced image processing tools. Therefore, under the title Hyperspectral image representation and Processing with Binary Partition Trees, this PhD thesis proposes the construction and the processing of a new region-based hierarchical hyperspectral image representation: the Binary Partition Tree (BPT). This hierarc...

  11. Pay schemes, bargaining, and competition for talent

    OpenAIRE

    Lindbeck, Assar; Weibull, Jörgen W.

    2015-01-01

    The paper provides a framework for analysis of remuneration to agents whose task is to make well-informed decisions on behalf of a principal, with managers in large corporations as the most prominent example. The principal and agent initially bargain over the pay scheme to the latter. The bargaining outcome depends both on competition for agents and on the relative bargaining power of the two parties, given their outside options, thus allowing for the possibility that the agent may be the cur...

  12. Trade flows analysis in textile sector between China, EU-28 and selected group of countries (2008-2014). A gravity model

    OpenAIRE

    Giménez Méndez, Jairo

    2016-01-01

    Treball Final de Grau en Economia. Codi: EC1049. Curs acadèmic 2015-2016 In an increasingly globalized world, political and economic relations between countries are of great importance. This article shows the existing business relationship between China and the European Union from 2008 to 2014. Thus focusing on the textile sector, we want to analyze the impact that have exports and Chinese trade flows on the countries of the European Union during the period of economic crisis t...

  13. Construction control of cable-stayed bridges

    OpenAIRE

    Lozano Galant, José Antonio

    2013-01-01

    Premi extraordinari doctorat curs 2012-2013, àmbit d’Enginyeria Civil This work presents a study of the simulation of cable-stayed bridges built on temporary supports focused on their response during construction and in service. To simulate the behavior during construction, a set of four different algorithms has been developed to deal with initial design (Backward Algorithm), updating the tensioning process when deviations on site are measured (Forward Algorithm), optimization processes (D...

  14. Padri della Chiesa e scrittori ecclesiastici occidentali (secoli II-XIII)

    OpenAIRE

    Guerrini, Mauro; Pieri, Paola

    2010-01-01

    ACOLIT is an authority list of catholic authors (persons or corporate bodies) and of liturgical and religious anonymus works. ACOLIT formulates catalogue entries on the basis of RICA, but also takes into account the Norme per il catalogo degli stampati of the Biblioteca Apostolica Vaticana, the AACR2R, the Regeln für die alphabetische Katalogisierung (RAK), the Reglas de catalogación, and the recommendations of IFLA. Three volumes have already been published: Bible, Catholic Church, Roman Cur...

  15. The influence of leadership in the working environment, teamwork and organisational learning : a theoretical review

    OpenAIRE

    Lacedón Montemayor, Marta

    2016-01-01

    Treball Final de Grau en Administració d'Empreses. Codi: AE1049. Curs: 2015/2016 The objective of this paper is to examine the influence that leadership has on creating a good working environment, on work teams and on organisational learning, through a theoretical revision. For this, concepts are addressed related to leadership such as the leader's profile, the role he represents within an organisation, his functions and skills, which will help us understand the importance of ...

  16. Matthew Jones House: Historic Maintenance and Repair Manual

    Science.gov (United States)

    2014-08-01

    space versus the closed-off drywalled space. This issue needs to be investigated; one suggestion would be to have two separate HVAC systems—one per...specialized space—to control the appropriate amount of humidity. Drywall has mold retention capabilities, therefore, some of it might have to be re...separates this room from the stair pas- sage. The ceiling joists have been covered with drywall . This room is cur- rently used as a viewing room for the

  17. Conservation and utilization of natural resources in the East Usambara forest reserves: conventional views and local perspectives

    OpenAIRE

    Kessy, J.F.

    1998-01-01

    The importance of conserving biological resources and the need for managing these resources for present and future generations has been given much attention internationally in the past three decades. These ideas have been emphasized in key documents such as the Brundtland report as well as in international conventions such as the global convention on biodiversity which followed the Rio meeting in 1992. The challenge in implementing these ideas lies in finding the proper trade-offs between cur...

  18. Finding Malicious Cyber Discussions in Social Media

    Science.gov (United States)

    2016-02-02

    reverse engineering, security, malware , blackhat) were labeled as cyber and posts on non- cyber topics (e.g., astronomy, electronics, beer, biology, mu...firewall, hash, infect, inject, install, key, malicious, malware , network, obfuscate, overflow, packet, password, payload, request, risk, scan, script...randomly sampled user discussing cyber topics, no extra labeling or cost is in- curred by incorporating additional training data. The same 127 cyber

  19. Definición y análisis de indicadores estratégicos para redes sociales : un caso de estudio en el sector automovilístico

    OpenAIRE

    Lanza Cruz, Indira Lázara

    2016-01-01

    Treball Final de Màster Universitari en Sistemes Intel·ligents. Codi: SIU043. Curs: 2015/2016 La disciplina Inteligencia de Negocios se dedica a definir indicadores estratégicos a partir de medidas de interés definidas sobre un conjunto de datos temporales recolectados desde diferentes fuentes, e integrados bajo un mismo esquema multidimensional. Tradicionalmente, los datos recolectados tienen un carácter corporativo (ventas, promociones, etc.) y son generados dentro de la mis...

  20. The teaching of the delivery procedure by the simulation method

    OpenAIRE

    2014-01-01

    M.Cur. (Advanced Midwifery and Neonatal Nursing) With the introduction of the four year comprehensive course, the students at a particular college of nursing receive instruction on normal labour at the beginning of their second year. They are then allocated to the maternity wards of a particular hospital for their midwifery nursing science practica. The practical placement of these students to the midwifery clinical area is variable as a few may be allocated to the practice immediately aft...

  1. Characterization and quantification of proteins secreted by single human embryos prior to implantation

    OpenAIRE

    Poli, Maurizio; Ori, Alessandro; Child, Tim; Jaroudi, Souraya; Spath, Katharina; Beck, Martin; Wells, Dagan

    2015-01-01

    The use of in?vitro fertilization (IVF) has revolutionized the treatment of infertility and is now responsible for 1?5% of all births in industrialized countries. During IVF, it is typical for patients to generate multiple embryos. However, only a small proportion of them possess the genetic and metabolic requirements needed in order to produce a healthy pregnancy. The identification of the embryo with the greatest developmental capacity represents a major challenge for fertility clinics. Cur...

  2. Využití staveb lidové architektury ve venkovském cestovním ruchu

    OpenAIRE

    Smejkalová, Šárka

    2014-01-01

    The topic of this bachelor thesis is The use of the monuments of folk architecture in rural tourism. The introduction deals with the characteristics of folk architecture, its history and regional types of rural monuments. The next part describes the protection of the monuments of folk architecture, their use in tourism and an overview of contemporary museums of folk architecture in the Czech Republic, focusing on the events. In the third part there is used SWOT analysis, evaluation of the cur...

  3. Caracterização nutricional e toxicológica de espécies de plantas silvestres. Análise mineral, antioxidante e de lectinas

    OpenAIRE

    Branco, Ana Carolina Franco Versos Brígida

    2015-01-01

    Mestrado em Engenharia Alimentar - Qualidade e Segurança Alimentar - Instituto Superior de Agronomia In the present work, leaves of four wild plants (Rumex crispus (curly dock), Sinapis arvensis (wild mustard), Beta maritima (sea beet) and Sonchus oleraceus (sow thistle)) were studied in order to analyse for their mineral composition, phenolic content, antioxidant capacity, and detection of lectins (anti-nutritive compound). The mineral and antioxidant study of different species was com...

  4. Psychological morbidity, sources of stress and coping strategies among undergraduate medical students of Nepal

    OpenAIRE

    Sreeramareddy, Chandrashekhar T; Shankar, Pathiyil R; Binu, VS; Mukhopadhyay, Chiranjoy; Ray, Biswabina; Menezes, Ritesh G

    2007-01-01

    Abstract Background In recent years there has been a growing appreciation of the issues of quality of life and stresses involved medical training as this may affect their learning and academic performance. However, such studies are lacking in medical schools of Nepal. Therefore, we carried out this study to assess the prevalence of psychological morbidity, sources and severity of stress and coping strategies among medical students in our integrated problem-stimulated undergraduate medical cur...

  5. Proposal of elaboration of the sustainability report to wine importer company in China

    OpenAIRE

    Franco López, Cristina

    2015-01-01

    Treball Final de Grau en Finances i Comptabilitat. Codi: FC1049. Curs acadèmic 2015-2016 Every time, the companies are more concerned with sustainability reports. This is due to the fact that the society is becoming aware with social, environmental and economic consequences. The society is changing their lifestyle and they are trying to change their actions to be more sustainable. The companies take a lot of decisions, and those could cause disasters because we live in a glo...

  6. Naval War College Review. Volume 67, Number 1, Winter 2014

    Science.gov (United States)

    2014-01-01

    professional. He is cur- rently engaged in advanced study at the assisi insti- tute of Brattleboro, vermont, in the area of archetyp - al pattern...thought processes. as Jung aptly noted, “in the West, consciousness has been developed mainly through science and technology—not through art, social...Fundamentals of war gaming, pp. 101–103. 17. Ibid., pp. 104–11. 18. Carl g. Jung , The earth Has a Soul: c. g. Jung on nature, Technology

  7. Evidences of methane-derived authigenic carbonates from the sediments of the Krishna–Godavari Basin, eastern continental margin of India

    Digital Repository Service at National Institute of Oceanography (India)

    Kocherla, M.; Mazumdar, A.; Karisiddaiah, S.M.; Borole, D.V.; Rao, B.R.

    mineral phases 10,11 , viz. calcite, aragonite, dol o- mite and siderite are brought about by the e n richment in pore water alkalinity. Enrichment in bicarbonate ion co n- centration has been attributed to a n aerobic oxidation of methane (AOM... cur as individual slabs, thinly lithified pavements, vertical pi l lars, mushroom - like structures, microbial mats, dispersed cry s tals an d as micro - concretions. The bicarbonate pool pr o duced as a result of bacterial anoxic methane...

  8. LEAD POISONING IN ANCIENT ROME 1. INTRODUCTION

    African Journals Online (AJOL)

    of organic lead compounds such as tetra-ethyl lead, found in modern times in leaded petrol.2 Significant absorption of lead can take place .... of the Empire period consumed 250 mg of lead a day, as against 35 mg for plebeians and 15 mg for slaves. He compares this with cur- rent figures of 30-50 mg lead a day for the ...

  9. Die gebruik van ontwikkelingsiftingskale vir gemeenskapsverpleegkunde

    OpenAIRE

    2014-01-01

    M.Cur. (General Nursing) Child development is a subject that is increasingly becoming the focus of discussion. The interest in this subject, however dates back to the twenties when Arnold Gesell started researching this field. Over the years scales were developed to make the measurement of the level of child development easier. The community health nurse should have a thorough knowledge of the use of child development scales. This will enable her to identify, diagnose and refer possible ca...

  10. Cellulose as an Architectural Element in Spatially Structured Escherichia coli Biofilms

    Science.gov (United States)

    Serra, Diego O.; Richter, Anja M.

    2013-01-01

    Morphological form in multicellular aggregates emerges from the interplay of genetic constitution and environmental signals. Bacterial macrocolony biofilms, which form intricate three-dimensional structures, such as large and often radially oriented ridges, concentric rings, and elaborate wrinkles, provide a unique opportunity to understand this interplay of “nature and nurture” in morphogenesis at the molecular level. Macrocolony morphology depends on self-produced extracellular matrix components. In Escherichia coli, these are stationary phase-induced amyloid curli fibers and cellulose. While the widely used “domesticated” E. coli K-12 laboratory strains are unable to generate cellulose, we could restore cellulose production and macrocolony morphology of E. coli K-12 strain W3110 by “repairing” a single chromosomal SNP in the bcs operon. Using scanning electron and fluorescence microscopy, cellulose filaments, sheets and nanocomposites with curli fibers were localized in situ at cellular resolution within the physiologically two-layered macrocolony biofilms of this “de-domesticated” strain. As an architectural element, cellulose confers cohesion and elasticity, i.e., tissue-like properties that—together with the cell-encasing curli fiber network and geometrical constraints in a growing colony—explain the formation of long and high ridges and elaborate wrinkles of wild-type macrocolonies. In contrast, a biofilm matrix consisting of the curli fiber network only is brittle and breaks into a pattern of concentric dome-shaped rings separated by deep crevices. These studies now set the stage for clarifying how regulatory networks and in particular c-di-GMP signaling operate in the three-dimensional space of highly structured and “tissue-like” bacterial biofilms. PMID:24097954

  11. Forced migration: a consequence of human rights violations: case study: Southern Cameroon

    OpenAIRE

    Tanjong, Ndeffor Bryan

    2014-01-01

    Treball final de Màster Universitari en Estudis internacionals de Pau, Conflictes i Desenvolupament. Codi SAA074. Curs acadèmic 2013/2014 Torture in particular is a complex trauma that often occurs within the context of widespread persecution and human rights violations. Modern welfare shows that whole populations are at risk of suffering extensive trauma, injustice loss and resultantly emigration- forced to migrate. Such has being the faith of Southern Cameroonians forced ...

  12. Figured grain in aspen is heritable and not affected by graft-transmissible signals

    Science.gov (United States)

    Youran Fan; Kendal Rupert; Alex C. Wiedenhoeft; Keith Woeste; Christian Lexer; Richard. Meilan

    2013-01-01

    Figure can add value to wood products, but its occurrence is unpredictable. A first step in reliably producing figured wood is determining whether it is faithfully transmitted to progeny via sexual and asexual reproduction. We describe a 26-year-old male aspen genotype, designated ‘Curly Poplar’, which was shown to be a Populus × canescens hybrid using microsatellite...

  13. Ceci est mon testament : Marc-Antoine Laugier

    OpenAIRE

    Restrepo Hernández, Fabio

    2011-01-01

    Premi extraordinari doctorat curs 2010-2011, àmbit d’Arquitectura, Urbanisme i Edificació The demolition the monolithic interpretation the engraving, which accompanies as Irontispiece Marc-Antoine Laugier's Essai sur l'Architecture, object the present work, aims to liberate the question about the origin architecture Irom the vicious circle created between the image the primitive hut and the myth the noble savage hidden within il. The engraving is without a doubt, the most ...

  14. La representación del trastorno de personalidad múltiple en "Shutter Island" y "El Club de la Lucha"

    OpenAIRE

    Olivares Alcántara, Ana

    2015-01-01

    Treball Final del Grau en Comunicació Audiovisual. Codi: CA0932. Curs: 2014/2015 Todo director de cine busca una historia única, una trama espectacular y, si puede ser, un giro inesperado de los acontecimientos para desestabilizar al espectador. El trastorno de identidad disociativo o trastorno de personalidad múltiple, como era conocido antes, permite crear una historia profunda para luego ser destruida y presentar la realidad de la trama, esto sorprende al espectador y le ...

  15. Analysis of the contribution of wind power plants to damp power system oscillations

    OpenAIRE

    Domínguez García, José Luis

    2013-01-01

    Premi extraordinari doctorat curs 2012-2013, àmbit d’Enginyeria Industrial Wind power has emerged as one of the most promising renewable energy sources. The very penetration levels of wind energy in power systems have altered several aspects of power system operation, such as system stability. Owing to the large penetration of wind power, transmission system operators (TSOs) have established special grid codes for wind farms connection. These grid codes require wind farms to provide ancill...

  16. JPRS Report, Science & Technology, Europe

    Science.gov (United States)

    1988-08-01

    Industrial R&D Project Funds [GAZZETTA UFFICIALE DELIA REPUBLICA ITALIANA, 13 Apr 88] 34 Trieste ’Scientific Pole’ Activities, Organization Reviewed...Italian Industrial R&D Project Funds 3698M355 Rome GAZZETTA UFFICIALE DELLA REPUBLICA ITALIANA in Italian No 86, 13 Apr 88 pp 11-19 [Under the...cur- rent news and information and is published Monday through Friday in 8 volumes: China , East Europe, Soviet Union, East Asia, Near East & South

  17. Système d'approvisionnement pharmaceutique au Maroc : opportunités et défaillances

    OpenAIRE

    TADLAOUI, KENZA; Anas, CHAFI; Abdelali, ENNADI

    2015-01-01

    International audience; Pharmaceuticals (PF) occupy an important place in the Moroccan health system. Indeed, medicines and medical goods alone absorbs 31.7% of total health spending. However, despite efforts to date in connection with the supply of PF in terms of savings made ( grouping of purchases) and quality assurance (European norms and standards), the availability of these products at health facilities is a concern. The patients do not fully benefit from these efforts.Moreover, the cur...

  18. European Scientific Notes. Volume 38, Number 8.

    Science.gov (United States)

    1984-08-01

    group’s Reference existence and its uniqueness with Doise -, W ., and S. Moscovici, eds., Cur- respect to other groups) influences at rent Issues in European...CAET M.A. Howard, USN August 1984 Scientific Director ..... James W . Daniel Volume 38 Editor .................. Larry E. Shaffer Number 8...for shallow- and deep-water research. SCIENCE POuCY 0 Holland’s Science Policy and Budget ..................... .James W . Daniel 438 Organizations

  19. Effect of curing time on microstructure and mechanical strength ...

    Indian Academy of Sciences (India)

    showed an increase in the total weight loss (%) with the cur- ing time. After 72 h of curing, the total weight loss achieves a maximum value of 19·17%. The increase in weight loss is related to the progress of geopolymerization process. The dissolution process in alkaline medium implies that Si–O–. Si, Al–O–Al and Si–O–Al ...

  20. CMS delegation for MTB55 held at Tata Institute in Mumbai (Bombay) during CMS week 14 December 2000.

    CERN Multimedia

    A. Payn

    2000-01-01

    CMS delegation at MTB55 in Tata Institue Mumbai on 14 December 2000. From left to right Bertrand Blau/ETHZ, Stefania Farinon/INFN-Genova, Alain Hervé/CERN, Lucien Veillet/CERN, Jean-Paul Grillet/CERN, Yves Pabot/Saclay, Bruno Levesy/Saclay, Alain Payn/Saclay, François Kircher/Saclay, Benoît Curé/CERN.