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Sample records for sweetpotato ipomoea batatas

  1. Rapid genetic transformation of sweetpotato ( Ipomoea batatas (L ...

    African Journals Online (AJOL)

    An efficient and rapid. Agrobacterium-mediated transformation method based on de novo (via callus) organogenesis has been developed from petioles with leaf for sweetpotato (Ipomoea batatas (L.) Lam). Stable transgenic sweetpotato plants cv. Jewel were obtained in six to ten weeks after infection with Agrobacterium ...

  2. Potentials of sweetpotato ( Ipomoea Batatas ) for confectionaries ...

    African Journals Online (AJOL)

    Sweetpotato roots were used as base for the preparation of confectioneries namely crisps, doughnuts, strips, chin-chin, bread, cakes biscuits, pancake, crunch and muffins. Sweetpotato starch was used to produce salad cream and the leaves were used to prepare stew and soup. Traditional foods including abacha, ncha, ...

  3. Effect of sweetpotato leaf (Ipomoea Batatas) extract on some ...

    African Journals Online (AJOL)

    The use of plants and herbs as food supplements and medicinal additives is fast gaining grounds and recognition in the world especially in Africa. Sweet potato leaves (Ipomoea batatas) decoction is a folk remedy for asthma, bugbites, burns, catarrh, diarrhea, fever, nausea, stomach distress and tumours. Also this plant has ...

  4. Cryopreservation of sweetpotato (Ipomoea batatas) and its pathogen eradication by cryotherapy.

    Science.gov (United States)

    Feng, Chaohong; Yin, Zhenfang; Ma, Yanli; Zhang, Zhibo; Chen, Long; Wang, Biao; Li, Baiquan; Huang, Yushen; Wang, Qiaochun

    2011-01-01

    Sweetpotato (Ipomoea batatas) ranks as the seventh most important staple crop in the world and the fifth in developing countries after rice, wheat, maize and cassava. Sweetpotato is mainly grown in developing countries, which account for more than 95% of total production of the whole world. Genetic resources, including cultivated varieties and wild species, are a prerequisite for novel sweetpotato breeding in both conventional and genetic engineering programs. Various cryopreservation protocols have been developed for shoot tips and embryogenic tissues. The former explants are preferred for long-term conservation of sweetpotato genetic resources, while the latter are valuable for sweetpotato genetic improvement. This review provides update comprehensive information on cryopreservation of sweetpotato shoot tips and embryogenic tissues. Plant pathogens such as viruses and phytoplasma severely hamper high yield and high quality production of sweetpotato. Thus, usage of pathogen-free planting materials is pivotal for sustainable sweetpotato production. Cryotherapy of shoot tips can efficiently eradicate sweetpotato pathogens such as viruses and phytoplasma. The mechanism behind pathogen eradication by cryotherapy of shoot tips has been elucidated. Pathogen eradication by cryotherapy provides an alternative, efficient strategy for production of pathogen-free plants. In addition, cryopreserved tissues may also be considered to be safer for exchange of germplasm between countries and regions. Copyright © 2010 Elsevier Inc. All rights reserved.

  5. Distributions, ex situ conservation priorities, and genetic resource potential of crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas

    NARCIS (Netherlands)

    Khoury, C.K.; Heider, B.; Castaneda-Alvarez, N.P.; Achicanoy, H.A.; Sosa, C.C.; Miller, R.E.; Scotland, R.W.; Wood, J.R.I.; Rossel, G.; Eserman, L.A.; Jarret, R.L.; Yencho, G.C.; Bernau, V.; Juarez, H.; Sotelo, S.; Haan, de S.; Struik, P.C.

    2015-01-01

    Crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, the limited availability of germplasm with which

  6. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas).

    Science.gov (United States)

    Wang, Zhangying; Li, Jun; Luo, Zhongxia; Huang, Lifei; Chen, Xinliang; Fang, Boping; Li, Yujun; Chen, Jingyi; Zhang, Xiongjian

    2011-10-20

    Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9%) being the most abundant followed by di- (41.2%), tetra- (9.2%), penta- (3.7%) and hexa-nucleotide (3.1%) repeat types. The top five motifs included AG/CT (26.9%), AAG/CTT (13.5%), AT/TA (10.6%), CCG/CGG (5.8%) and AAT/ATT (4.5%). After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30) and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9%) and 342 (41.9%) primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would be useful for qualitative and quantitative

  7. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Li Yujun

    2011-10-01

    Full Text Available Abstract Background Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas, which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. Results In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9% being the most abundant followed by di- (41.2%, tetra- (9.2%, penta- (3.7% and hexa-nucleotide (3.1% repeat types. The top five motifs included AG/CT (26.9%, AAG/CTT (13.5%, AT/TA (10.6%, CCG/CGG (5.8% and AAT/ATT (4.5%. After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30 and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9% and 342 (41.9% primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. Conclusion This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would

  8. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas)

    Science.gov (United States)

    2011-01-01

    Background Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. Results In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9%) being the most abundant followed by di- (41.2%), tetra- (9.2%), penta- (3.7%) and hexa-nucleotide (3.1%) repeat types. The top five motifs included AG/CT (26.9%), AAG/CTT (13.5%), AT/TA (10.6%), CCG/CGG (5.8%) and AAT/ATT (4.5%). After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30) and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9%) and 342 (41.9%) primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. Conclusion This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would be useful for

  9. Demographic comparison of sweetpotato weevil reared on a major host, Ipomoea batatas, and an alternative host, I. triloba

    OpenAIRE

    Gadi V. P. Reddy; Hisn Chi

    2015-01-01

    In this study, we collected life table data for the sweetpotato weevil, Cylas formicarius, grown on Ipomoea batatas and Ipomoea triloba, and analyzed them using an age-stage, two-sex life table. We also demonstrated the growth potential of C. formicarius on these two host plants by using population projection. These data will be useful to the growers to the selection or eradication of host plants in an integrated control strategy for C. formicarius for the entire area of the targeted areas. W...

  10. Ipomoea Batatas

    African Journals Online (AJOL)

    Tope

    Sweet potato leaves (Ipomoea batatas) decoction is a folk remedy for asthma, bugbites, burns, catarrh, diarrhea, fever, nausea, stomach distress and tumours. Also this plant has haematinic effects and has been used in the treatment of anaemia and other related ailments. Sweet potato leaves were used to feed rabbits.

  11. Demographic comparison of sweetpotato weevil reared on a major host, Ipomoea batatas, and an alternative host, I. triloba.

    Science.gov (United States)

    Reddy, Gadi V P; Chi, Hisn

    2015-07-09

    In this study, we collected life table data for the sweetpotato weevil, Cylas formicarius, grown on Ipomoea batatas and Ipomoea triloba, and analyzed them using an age-stage, two-sex life table. We also demonstrated the growth potential of C. formicarius on these two host plants by using population projection. These data will be useful to the growers to the selection or eradication of host plants in an integrated control strategy for C. formicarius for the entire area of the targeted areas. We found that C. formicarius developed faster on I. batatas than on I. triloba. The developmental times of the larval and pupal stages on I. batatas than on I. triloba were 37.01 and 8.3 days. The adult females emerged before and began to produce eggs at 42 days earlier when reared on I. batatas. The fecundity of females was 90.0 eggs on I. batatas significantly higher than the mean fecundity of 68.5 eggs on I. triloba. Although this insect has a higher intrinsic rate of increase on I. batatas, the study indicated that C. formicarius can successfully survive and reproduce on both host plants.

  12. Demographic comparison of sweetpotato weevil reared on a major host, Ipomoea batatas, and an alternative host, I. triloba

    Science.gov (United States)

    Reddy, Gadi V. P.; Chi, Hisn

    2015-01-01

    In this study, we collected life table data for the sweetpotato weevil, Cylas formicarius, grown on Ipomoea batatas and Ipomoea triloba, and analyzed them using an age-stage, two-sex life table. We also demonstrated the growth potential of C. formicarius on these two host plants by using population projection. These data will be useful to the growers to the selection or eradication of host plants in an integrated control strategy for C. formicarius for the entire area of the targeted areas. We found that C. formicarius developed faster on I. batatas than on I. triloba. The developmental times of the larval and pupal stages on I. batatas than on I. triloba were 37.01 and 8.3 days. The adult females emerged before and began to produce eggs at 42 days earlier when reared on I. batatas. The fecundity of females was 90.0 eggs on I. batatas significantly higher than the mean fecundity of 68.5 eggs on I. triloba. Although this insect has a higher intrinsic rate of increase on I. batatas, the study indicated that C. formicarius can successfully survive and reproduce on both host plants. PMID:26156566

  13. Carotenoids gene markers for sweetpotato (Ipomoea batatas L. Lam): applications in genetic mapping, diversity evaluation and cross-species transference.

    Science.gov (United States)

    Arizio, C M; Costa Tártara, S M; Manifesto, M M

    2014-04-01

    Carotenoids play essential biological roles in plants, and genes involved in the carotenoid biosynthesis pathway are evolutionarily conserved. Orange sweetpotato is an important source of β-carotene, a precursor of vitamin A. In spite of this, only a few research studies have focussed on the molecular aspects of carotenoid genes regarding their specific sequence and structure. In this study, we used published carotenoid gene sequences from Ipomoea and other species for "exon-primed intron-crossing" approaches. Fifteen pairs of primers representing six carotenoid genes were designed for different introns, eleven of which amplified scorable and reproducible alleles. The sequence of PCR products showed high homology to the original ones. Moreover, the structure and sequence of the introns and exons from five carotenoid structural genes were partially defined. Intron length polymorphism and intron single nucleotide polymorphisms were detected in amplified sequences. Marker dosages and allelic segregations were analysed in a mapping population. The developed markers were evaluated in a set of Ipomoeas batatas accessions so as to analyse genetic diversity and conservation applicability. Using CG strategy combined with EPIC-PCR technique, we developed carotenoid gene markers in sweetpotato. We reported the first set of polymorphic Candidate Gene markers for I. batatas, and demonstrated transferability in seven wild Ipomoea species. We described the sequence and structure of carotenoid genes and introduced new information about genomic constitution and allele dosage.

  14. A genome-wide BAC-end sequence survey provides first insights into sweetpotato (Ipomoea batatas (L.) Lam.) genome composition.

    Science.gov (United States)

    Si, Zengzhi; Du, Bing; Huo, Jinxi; He, Shaozhen; Liu, Qingchang; Zhai, Hong

    2016-11-21

    Sweetpotato, Ipomoea batatas (L.) Lam., is an important food crop widely grown in the world. However, little is known about the genome of this species because it is a highly heterozygous hexaploid. Gaining a more in-depth knowledge of sweetpotato genome is therefore necessary and imperative. In this study, the first bacterial artificial chromosome (BAC) library of sweetpotato was constructed. Clones from the BAC library were end-sequenced and analyzed to provide genome-wide information about this species. The BAC library contained 240,384 clones with an average insert size of 101 kb and had a 7.93-10.82 × coverage of the genome, and the probability of isolating any single-copy DNA sequence from the library was more than 99%. Both ends of 8310 BAC clones randomly selected from the library were sequenced to generate 11,542 high-quality BAC-end sequences (BESs), with an accumulative length of 7,595,261 bp and an average length of 658 bp. Analysis of the BESs revealed that 12.17% of the sweetpotato genome were known repetitive DNA, including 7.37% long terminal repeat (LTR) retrotransposons, 1.15% Non-LTR retrotransposons and 1.42% Class II DNA transposons etc., 18.31% of the genome were identified as sweetpotato-unique repetitive DNA and 10.00% of the genome were predicted to be coding regions. In total, 3,846 simple sequences repeats (SSRs) were identified, with a density of one SSR per 1.93 kb, from which 288 SSRs primers were designed and tested for length polymorphism using 20 sweetpotato accessions, 173 (60.07%) of them produced polymorphic bands. Sweetpotato BESs had significant hits to the genome sequences of I. trifida and more matches to the whole-genome sequences of Solanum lycopersicum than those of Vitis vinifera, Theobroma cacao and Arabidopsis thaliana. The first BAC library for sweetpotato has been successfully constructed. The high quality BESs provide first insights into sweetpotato genome composition, and have significant hits to the genome

  15. Expression of class I knotted1-like homeobox genes in the storage roots of sweetpotato (Ipomoea batatas).

    Science.gov (United States)

    Tanaka, Masaru; Kato, Nakao; Nakayama, Hiroki; Nakatani, Makoto; Takahata, Yasuhiro

    2008-11-01

    As a first step in clarifying the involvement of class I knotted1-like homeobox (KNOXI) genes in the storage root development of sweetpotato (Ipomoea batatas), we isolated three KNOXI genes, named Ibkn1, Ibkn2 and Ibkn3, expressed in the storage roots. Phylogenetic analysis showed that Ibkn1 was homologous to the SHOOT MERISTEMLESS (STM) gene of Arabidopsis, while Ibkn2 and Ibkn3 were homologous to the BREVIPEDICELLUS (BP) gene. Of these, expression of Ibkn1 and Ibkn2 were upregulated in developing and mature storage roots compared with fibrous roots. Ibkn1 and Ibkn2 showed different expression patterns in the storage roots. Ibkn1 was preferentially expressed at the proximal end and around the primary vascular cambium, while Ibkn2 expression was highest in the thickest part and lower in both the proximal and distal ends. In contrast to Ibkn1 and Ibkn2, expression of Ibkn3 in roots was not consistent among sweetpotato cultivars. The distribution of endogenous trans-zeatin riboside (t-ZR) in sweetpotato roots showed a similarity to the expression pattern of KNOXI genes, supporting the idea that KNOXI genes control cytokinin levels in the storage roots. The physiological functions of these KNOXI genes in storage root development are discussed.

  16. A high-density SNP genetic map consisting of a complete set of homologous groups in autohexaploid sweetpotato (Ipomoea batatas).

    Science.gov (United States)

    Shirasawa, Kenta; Tanaka, Masaru; Takahata, Yasuhiro; Ma, Daifu; Cao, Qinghe; Liu, Qingchang; Zhai, Hong; Kwak, Sang-Soo; Cheol Jeong, Jae; Yoon, Ung-Han; Lee, Hyeong-Un; Hirakawa, Hideki; Isobe, Sachiko

    2017-03-10

    Sweetpotato (Ipomoea batatas) is an autohexaploid species with 90 chromosomes (2n = 6x = 90) and a basic chromosome number of 15, and is therefore regarded as one of the most challenging species for high-density genetic map construction. Here, we used single nucleotide polymorphisms (SNPs) identified by double-digest restriction site-associated DNA sequencing based on next-generation sequencing technology to construct a map for sweetpotato. We then aligned the sequence reads onto the reference genome sequence of I. trifida, a likely diploid ancestor of sweetpotato, to detect SNPs. In addition, to simplify analysis of the complex genetic mode of autohexaploidy, we used an S1 mapping population derived from self-pollination of a single parent. As a result, 28,087 double-simplex SNPs showing a Mendelian segregation ratio in the S1 progeny could be mapped onto 96 linkage groups (LGs), covering a total distance of 33,020.4 cM. Based on the positions of the SNPs on the I. trifida genome, the LGs were classified into 15 groups, each with roughly six LGs and six small extra groups. The molecular genetic techniques used in this study are applicable to high-density mapping of other polyploid plant species, including important crops.

  17. A high-density SNP genetic map consisting of a complete set of homologous groups in autohexaploid sweetpotato (Ipomoea batatas)

    Science.gov (United States)

    Shirasawa, Kenta; Tanaka, Masaru; Takahata, Yasuhiro; Ma, Daifu; Cao, Qinghe; Liu, Qingchang; Zhai, Hong; Kwak, Sang-Soo; Cheol Jeong, Jae; Yoon, Ung-Han; Lee, Hyeong-Un; Hirakawa, Hideki; Isobe, Sachiko

    2017-01-01

    Sweetpotato (Ipomoea batatas) is an autohexaploid species with 90 chromosomes (2n = 6x = 90) and a basic chromosome number of 15, and is therefore regarded as one of the most challenging species for high-density genetic map construction. Here, we used single nucleotide polymorphisms (SNPs) identified by double-digest restriction site-associated DNA sequencing based on next-generation sequencing technology to construct a map for sweetpotato. We then aligned the sequence reads onto the reference genome sequence of I. trifida, a likely diploid ancestor of sweetpotato, to detect SNPs. In addition, to simplify analysis of the complex genetic mode of autohexaploidy, we used an S1 mapping population derived from self-pollination of a single parent. As a result, 28,087 double-simplex SNPs showing a Mendelian segregation ratio in the S1 progeny could be mapped onto 96 linkage groups (LGs), covering a total distance of 33,020.4 cM. Based on the positions of the SNPs on the I. trifida genome, the LGs were classified into 15 groups, each with roughly six LGs and six small extra groups. The molecular genetic techniques used in this study are applicable to high-density mapping of other polyploid plant species, including important crops. PMID:28281636

  18. Distributions, ex situ conservation priorities, and genetic resource potential of crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas].

    Science.gov (United States)

    Khoury, Colin K; Heider, Bettina; Castañeda-Álvarez, Nora P; Achicanoy, Harold A; Sosa, Chrystian C; Miller, Richard E; Scotland, Robert W; Wood, John R I; Rossel, Genoveva; Eserman, Lauren A; Jarret, Robert L; Yencho, G C; Bernau, Vivian; Juarez, Henry; Sotelo, Steven; de Haan, Stef; Struik, Paul C

    2015-01-01

    Crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, the limited availability of germplasm with which to perform crosses, and the difficulty of introgression of genes from wild species has constrained their utilization. Here, we compile geographic occurrence data on relevant sweetpotato wild relatives and produce potential distribution models for the species. We then assess the comprehensiveness of ex situ germplasm collections, contextualize these results with research and breeding priorities, and use ecogeographic information to identify species with the potential to contribute desirable agronomic traits. The fourteen species that are considered the closest wild relatives of sweetpotato generally occur from the central United States to Argentina, with richness concentrated in Mesoamerica and in the extreme Southeastern United States. Currently designated species differ among themselves and in comparison to the crop in their adaptations to temperature, precipitation, and edaphic characteristics and most species also show considerable intraspecific variation. With 79% of species identified as high priority for further collecting, we find that these crop genetic resources are highly under-represented in ex situ conservation systems and thus their availability to breeders and researchers is inadequate. We prioritize taxa and specific geographic locations for further collecting in order to improve the completeness of germplasm collections. In concert with enhanced conservation of sweetpotato wild relatives, further taxonomic research, characterization and evaluation of germplasm, and improving the techniques to overcome barriers to introgression with wild species are needed in order to mobilize these genetic resources for crop breeding.

  19. Distributions, ex situ conservation priorities, and genetic resource potential of crop wild relatives of sweetpotato [Ipomoea batatas (L. Lam., I. series Batatas

    Directory of Open Access Journals (Sweden)

    Colin Kahlil Khoury

    2015-04-01

    Full Text Available Crop wild relatives of sweetpotato [Ipomoea batatas (L. Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, the limited availability of germplasm with which to perform crosses, and the difficulty of introgression of genes from wild species has constrained their utilization. Here we compile geographic occurrence data on relevant sweetpotato wild relatives and produce potential distribution models for the species. We then assess the comprehensiveness of ex situ germplasm collections, contextualize these results with research and breeding priorities, and use ecogeographic information to identify species with the potential to contribute desirable agronomic traits. The fourteen species that are considered the closest wild relatives of sweetpotato generally occur from the central United States to Argentina, with richness concentrated in Mesoamerica and in the extreme southeastern United States. Currently designated species differ among themselves and in comparison to the crop in their adaptations to temperature, precipitation, and edaphic characteristics and most species also show considerable intraspecific variation. With 79% of species identified as high priority for further collecting, we find that these crop genetic resources are highly under-represented in ex situ conservation systems and thus their availability to breeders and researchers is inadequate. We prioritize taxa and specific geographic locations for further collecting in order to improve the completeness of germplasm collections. In concert with enhanced conservation of sweetpotato wild relatives, further taxonomic research, characterization and evaluation of germplasm, and improving the techniques to overcome barriers to introgression with wild species are needed in order to mobilize these genetic resources for crop breeding.

  20. Distributions, ex situ conservation priorities, and genetic resource potential of crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas

    Science.gov (United States)

    Khoury, Colin K.; Heider, Bettina; Castañeda-Álvarez, Nora P.; Achicanoy, Harold A.; Sosa, Chrystian C.; Miller, Richard E.; Scotland, Robert W.; Wood, John R. I.; Rossel, Genoveva; Eserman, Lauren A.; Jarret, Robert L.; Yencho, G. C.; Bernau, Vivian; Juarez, Henry; Sotelo, Steven; de Haan, Stef; Struik, Paul C.

    2015-01-01

    Crop wild relatives of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, the limited availability of germplasm with which to perform crosses, and the difficulty of introgression of genes from wild species has constrained their utilization. Here, we compile geographic occurrence data on relevant sweetpotato wild relatives and produce potential distribution models for the species. We then assess the comprehensiveness of ex situ germplasm collections, contextualize these results with research and breeding priorities, and use ecogeographic information to identify species with the potential to contribute desirable agronomic traits. The fourteen species that are considered the closest wild relatives of sweetpotato generally occur from the central United States to Argentina, with richness concentrated in Mesoamerica and in the extreme Southeastern United States. Currently designated species differ among themselves and in comparison to the crop in their adaptations to temperature, precipitation, and edaphic characteristics and most species also show considerable intraspecific variation. With 79% of species identified as high priority for further collecting, we find that these crop genetic resources are highly under-represented in ex situ conservation systems and thus their availability to breeders and researchers is inadequate. We prioritize taxa and specific geographic locations for further collecting in order to improve the completeness of germplasm collections. In concert with enhanced conservation of sweetpotato wild relatives, further taxonomic research, characterization and evaluation of germplasm, and improving the techniques to overcome barriers to introgression with wild species are needed in order to mobilize these genetic resources for crop breeding. PMID:25954286

  1. Comparative analysis of the root transcriptomes of cultivated sweetpotato (Ipomoea batatas [L.] Lam) and its wild ancestor (Ipomoea trifida [Kunth] G. Don).

    Science.gov (United States)

    Ponniah, Sathish K; Thimmapuram, Jyothi; Bhide, Ketaki; Kalavacharla, Venu Kal; Manoharan, Muthusamy

    2017-01-13

    The complex process of formation of storage roots (SRs) from adventitious roots affects sweetpotato yield. Identifying the genes that are uniquely expressed in the SR forming cultivated species, Ipomoea batatas (Ib), and its immediate ancestral species, Ipomoea trifida (It), which does not form SRs, may provide insights into the molecular mechanisms underlying SR formation in sweetpotato. Illumina paired-end sequencing generated ~208 and ~200 million reads for Ib and It, respectively. Trinity assembly of the reads resulted in 98,317 transcripts for Ib and 275,044 for It, after post-assembly removal of trans-chimeras. From these sequences, we identified 4,865 orthologous genes in both Ib and It, 60 paralogous genes in Ib and 2,286 paralogous genes in It. Among paralogous gene sets, transcripts encoding the transcription factor RKD, which may have a role in nitrogen regulation and starch formation, and rhamnogalacturonate lyase (RGL) family proteins, which produce the precursors of cell wall polysaccharides, were found only in Ib. In addition, transcripts encoding a K + efflux antiporter (KEA5) and the ERECTA protein kinase, which function in phytohormonal regulation and root proliferation, respectively, were also found only in Ib. qRT-PCR indicated that starch and sucrose metabolism genes, such as those encoding ADP-glucose pyrophosphorylase and beta-amylase, showed lower expression in It than Ib, whereas lignin genes such as caffeoyl-CoA O-methyltransferase (CoMT) and cinnamyl alcohol dehydrogenase (CAD) showed higher expression in It than Ib. A total of 7,067 and 9,650 unique microsatellite markers, 1,037,396 and 495,931 single nucleotide polymorphisms (SNPs) and 103,439 and 69,194 InDels in Ib and It, respectively, were also identified from this study. The detection of genes involved in the biosynthesis of RGL family proteins, the transcription factor RKD, and genes encoding a K + efflux antiporter (KEA5) and the ERECTA protein kinase only in I. batatas indicate

  2. A comparative study of proteomic differences between pencil and storage roots of sweetpotato (Ipomoea batatas (L.) Lam.).

    Science.gov (United States)

    Lee, Jeung Joo; Kim, Yun-Hee; Kwak, Youn-Sig; An, Jae Young; Kim, Pil Joo; Lee, Byung Hyun; Kumar, Vikranth; Park, Kee Woong; Chang, Eun Sil; Jeong, Jae Cheol; Lee, Haeng-Soon; Kwak, Sang-Soo

    2015-02-01

    Fibrous roots of sweetpotato (Ipomoea batatas (L.) Lam.) usually develop into both pencil and storage roots. To understand protein function in root development, a proteomic analysis was conducted on the pencil and storage roots of the light orange-fleshed sweetpotato cultivar, Yulmi. Two-dimensional gel electrophoresis showed that expression of 30 protein spots differed between pencil and storage roots: 15 proteins were up-regulated or expressed in pencil roots and 15 in storage roots. Differentially expressed proteins spots were investigated using matrix-assisted laser desorption/ionization time of flight mass spectrometry, and 10 proteins from pencil roots were identified as binding protein isoform A, catechol oxidase, peroxidases, ascorbate peroxidase, endochitinase, flavanone 3-hydroxylase and unknown proteins. Of the proteins up-regulated in, or restricted to, storage roots, 13 proteins were identified as protein disulfide isomerase, anionic peroxidase, putative ripening protein, sporamin B, sporamin A and sporamin A precursor. An analysis of enzyme activity revealed that catechol oxidase and peroxidase as the first and last enzymes of the lignin biosynthesis pathway, and ascorbate peroxidase had higher activities in pencil than in storage roots. The total concentration of phenolic compounds was also far higher in pencil than in storage roots, and lignin accumulated only in pencil roots. These results provide important insight into sweetpotato proteomics, and imply that lignin biosynthesis and stress-related proteins are up-regulated or uniquely expressed in pencil roots. The results indicate that the reduction of carbon flow toward phenylpropanoid biosynthesis and its delivery to carbohydrate metabolism is a major event in storage root formation. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  3. Carotenoid analysis of sweetpotato Ipomoea batatas and functional identification of its lycopene β- and ε-cyclase genes.

    Science.gov (United States)

    Khan, Muhammad Zubair; Takemura, Miho; Maoka, Takashi; Otani, Motoyasu; Misawa, Norihiko

    2016-09-01

    Sweetpotato Ipomoea batatas is known as a hexaploid species. Here, we analyzed carotenoids contained in the leaves and tubers of sweetpotato cultivars 'White Star' (WS) and W71. These cultivars were found to contain several carotenoids unique to sweetpotato tubers such as β-carotene-5,6,5',8'-diepoxide and β-carotene-5,8-epoxide. Next, we isolated two kinds of carotene cyclase genes that encode lycopene β- and ε-cyclases from the WS and W71 leaves, by RT-PCR and subsequent RACE. Two and three lycopene β-cyclase gene sequences were, respectively, isolated from WS, named IbLCYb1, 2, and from W71, IbLCYb3, 4, 5. Meanwhile, only a single lycopene ε-cyclase gene sequence, designated IbLCYe, was isolated from both WS and W71. These genes were separately introduced into a lycopene-synthesizing Escherichia coli transformed with the Pantoea ananatis crtE, crtB and crtI genes, followed by HPLC analysis. β-Carotene was detected in E. coli cells that carried IbLCYb1-4, indicating that the IbLCYb1-4 genes encode lycopene β-cyclase. Meanwhile, the introduction of IbLCYe into the lycopene-synthesizing E. coli led to efficient production of δ-carotene with a monocyclic ε-ring, providing evidence that the IbLCYe gene codes for lycopene ε-(mono)cyclase. Expression of the β- and ε-cyclase genes was analyzed as well.

  4. Antibacterial Activity of Sweetpotato (Ipomoea batatas L.) Fiber on Food Hygienic Bacteria

    OpenAIRE

    吉元, 誠; 木戸, めぐみ; 倉田, 理恵; 小林, 透; ヨシモト, マコト; キド, メグミ; クラタ, リエ; コバヤシ, トオル; Makoto, YOSHIMOTO; Megumi, KIDO; Rie, KURATA; Tooru, KOBAYASHI

    2011-01-01

    The antibacterial activity of sweetpotato fiber against pathogenic Escherichia coli (O157:H7), Salmonella typhimurium, Staphylococcus aureus, and Saccharomyces cerevisiae was investigated using microcalorimetry. The fiber enzymatically prepared from three varieties of sweetpotato storage roots (Koganesengan, Shiroyutaka, and Kyushu No. 124) exhibited bacteriostatic activity against pathogenic E. coli, S. typhimurium, and S. aureus; however, commercial fiber (Satsumaimo fiber) after citric aci...

  5. Comparison of proteomic and metabolomic profiles of two contrasting ecotypes of sweetpotato (Ipomoea batata L.).

    Science.gov (United States)

    Shekhar, Shubhendu; Mishra, Divya; Gayali, Saurabh; Buragohain, Alak Kumar; Chakraborty, Subhra; Chakraborty, Niranjan

    2016-06-30

    Sweetpotato has long been acknowledged as a significant contributor of global caloric needs, which continues to be of remarkable economic value. It is an important staple and emergency food in many countries and its annual world production hovers to about 130 million tons. The tubers act as sink and compete for the available photoassimilates eventually leading to the acquisition of nutrients and phytochemicals. Differential display of genes or gene-products, and metabolites causes differences in nutritive value of closely related ecotypes. To better understand the molecular basis for differential nutrient availability and phytochemicals, and exploit the natural genetic variation(s), we aimed at developing proteometabolic profiles of two contrasting ecotypes of sweetpotato. Proteomic analyses led to the identification of 1541 and 1201 proteins in orange fleshed and white fleshed sweetpotato ecotypes, respectively, presumably associated with binding, followed by catalytic, transferase, hydrolase, kinase and transporter activities. Furthermore, metabolome profiling revealed 148 and 126 metabolites in cv. OFSP and WFSP, respectively. This study would provide a basis for future comparative proteometabolomic efforts for sweetpotato, in particular and tuber crops in general. The results would expand our understanding of the proteome as well as metabolome and give new insights into how ecotype-specific traits are developed. Sweetpotato, the potato of the tropics, is the seventh most important crop worldwide in terms of production for food and additional industrial resources. Over 95% of the global sweet potato is produced in developing countries where it is considered as emergency food. It is also a vegetable, a snack food and confectionery item in most countries. It greatly contributes as a phytochemical source of nutrition and can produce more edible energy per hectare per day than wheat or rice. The adaptability to a wide range of agroecological conditions with least

  6. Temporal patterns of gene expression associated with tuberous root formation and development in sweetpotato (Ipomoea batatas).

    Science.gov (United States)

    Wang, Zhangying; Fang, Boping; Chen, Xinliang; Liao, Minghuan; Chen, Jingyi; Zhang, Xiongjian; Huang, Lifei; Luo, Zhongxia; Yao, Zhufang; Li, Yujun

    2015-07-16

    The tuberous root of sweetpotato is undisputedly an important organ from agronomic and biological perspectives. Little is known regarding the regulatory networks programming tuberous root formation and development. Here, as a first step toward understanding these networks, we analyzed and characterized the genome-wide transcriptional profiling and dynamics of sweetpotato root in seven distinct developmental stages using a customized microarray containing 39,724 genes. Analysis of these genes identified temporal programs of gene expression, including hundreds of transcription factor (TF) genes. We found that most genes active in roots were shared across all developmental stages, although significant quantitative changes in gene abundance were observed for 5,368 (including 435 TFs) genes. Clustering analysis of these differentially expressed genes pointed out six distinct expression patterns during root development. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that genes involved in different processes were enriched at specific stages of root development. In contrast with the large number of shared expressed genes in root development, each stage or period of root development has only a small number of specific genes. In total, 712 (including 27 TFs) and 1,840 (including 115 TFs) genes were identified as root-stage and root-period specific, respectively at the level of microarray. Several of the specific TF genes are known regulators of root development, including DA1-related protein, SHORT-ROOT and BEL1-like. The remaining TFs with unknown roles would also play critical regulatory roles during sweetpotato tuberous root formation and development. The results generated in this study provided spatiotemporal patterns of root gene expression in support of future efforts for understanding the underlying molecular mechanism that control sweetpotato yield and quality.

  7. Adventitious root primordia formation and development in stem nodes of 'Georgia Jet' sweetpotato, Ipomoea batatas.

    Science.gov (United States)

    Ma, Jun; Aloni, Roni; Villordon, Arthur; Labonte, Don; Kfir, Yanir; Zemach, Hanita; Schwartz, Amnon; Althan, Leviah; Firon, Nurit

    2015-07-01

    • Yield in sweetpotato is determined by the number of storage roots produced per plant. Storage roots develop from adventitious roots (ARs) present in stem cuttings that serve as propagation material. Data on the origin of sweetpotato ARs and the effect of nodal position on AR establishment and further development are limited.• We anatomically described root primordium initiation using stem sections and measured number of root primordia formed at different nodal positions using light microscopy and correlated nodal positions with AR number and length 14 d after planting (DAP).• Primordia for ARs initiate at the junction of the stem pith ray and the cambium, on both sides of the leaf gap, and they are well developed before emerging from the stem. The number of ARs that develop from isolated stem nodes 14 DAP corresponded to the number of AR primordia detected inside the stem. The total length of established roots at nodes 9-13 from the apex is about 2-fold longer than at nodes 5-8.• Nodal position (age) has a significant effect on the developmental status and number of root primordia inside the stem, determining the number and length of ARs that have developed by 14 DAP. Adventitious roots originating from nodes 9-13 possess similar AR systems and develop better than those originating from younger nodes 3-8. The mechanism regulating AR initiation in nodes is discussed. This system can serve for studying the effect of environmental conditions on AR initiation, development, and capacity to form storage roots. © 2015 Botanical Society of America, Inc.

  8. SRD1 is involved in the auxin-mediated initial thickening growth of storage root by enhancing proliferation of metaxylem and cambium cells in sweetpotato (Ipomoea batatas).

    Science.gov (United States)

    Noh, Seol Ah; Lee, Haeng-Soon; Huh, Eun Joo; Huh, Gyung Hye; Paek, Kyung-Hee; Shin, Jeong Sheop; Bae, Jung Myung

    2010-03-01

    A sweetpotato (Ipomoea batatas cv. 'Jinhongmi') MADS-box protein cDNA (SRD1) has been isolated from an early stage storage root cDNA library. The role of the SRD1 gene in the formation of the storage root in sweetpotato was investigated by an expression pattern analysis and characterization of SRD1-overexpressing (ox) transgenic sweetpotato plants. Transcripts of SRD1 were detected only in root tissues, with the fibrous root having low levels of the transcript and the young storage root showing relatively higher transcript levels. SRD1 mRNA was mainly found in the actively dividing cells, including the vascular and cambium cells of the young storage root. The transcript level of SRD1 in the fibrous roots increased in response to 1000 muM indole-3-acetic acid (IAA) applied exogenously. During the early stage of storage root development, the endogenous IAA content and SRD1 transcript level increased concomitantly, suggesting an involvement of SRD1 during the early stage of the auxin-dependent development of the storage root. SRD1-ox sweetpotato plants cultured in vitro produced thicker and shorter fibrous roots than wild-type plants. The metaxylem and cambium cells of the fibrous roots of SRD1-ox plants showed markedly enhanced proliferation, resulting in the fibrous roots of these plants showing an earlier thickening growth than those of wild-type plants. Taken together, these results demonstrate that SRD1 plays a role in the formation of storage roots by activating the proliferation of cambium and metaxylem cells to induce the initial thickening growth of storage roots in an auxin-dependent manner.

  9. SRD1 is involved in the auxin-mediated initial thickening growth of storage root by enhancing proliferation of metaxylem and cambium cells in sweetpotato (Ipomoea batatas)

    Science.gov (United States)

    Noh, Seol Ah; Lee, Haeng-Soon; Huh, Eun Joo; Huh, Gyung Hye; Paek, Kyung-Hee; Shin, Jeong Sheop; Bae, Jung Myung

    2010-01-01

    A sweetpotato (Ipomoea batatas cv. ‘Jinhongmi’) MADS-box protein cDNA (SRD1) has been isolated from an early stage storage root cDNA library. The role of the SRD1 gene in the formation of the storage root in sweetpotato was investigated by an expression pattern analysis and characterization of SRD1-overexpressing (ox) transgenic sweetpotato plants. Transcripts of SRD1 were detected only in root tissues, with the fibrous root having low levels of the transcript and the young storage root showing relatively higher transcript levels. SRD1 mRNA was mainly found in the actively dividing cells, including the vascular and cambium cells of the young storage root. The transcript level of SRD1 in the fibrous roots increased in response to 1000 μM indole-3-acetic acid (IAA) applied exogenously. During the early stage of storage root development, the endogenous IAA content and SRD1 transcript level increased concomitantly, suggesting an involvement of SRD1 during the early stage of the auxin-dependent development of the storage root. SRD1-ox sweetpotato plants cultured in vitro produced thicker and shorter fibrous roots than wild-type plants. The metaxylem and cambium cells of the fibrous roots of SRD1-ox plants showed markedly enhanced proliferation, resulting in the fibrous roots of these plants showing an earlier thickening growth than those of wild-type plants. Taken together, these results demonstrate that SRD1 plays a role in the formation of storage roots by activating the proliferation of cambium and metaxylem cells to induce the initial thickening growth of storage roots in an auxin-dependent manner. PMID:20150515

  10. In vivo anti-fatigue activity of total flavonoids from sweetpotato [Ipomoea batatas (L.) Lam.] leaf in mice.

    Science.gov (United States)

    Li, Chunguang; Zhang, Lianying

    2013-08-01

    The in vivo anti-fatigue activity of the total flavonoids from sweet potato [Ipomoea batatas (L.) Lam.] leaf was investigated in male Kunming mice. The total flavonoids from sweet potato leaf (TFSL) were orally administered at doses of 50, 100 and 200 mg/kg for 4 weeks and the anti-fatigue effect was studied using a weight-loaded swimming test, along with the determination of serum urea nitrogen (SUN), blood lactic acid (BLA) and hepatic and muscle glycogen contents. The results showed that TFSL had significant anti-fatigue effects. TFSL extended the exhaustive swimming time, effectively inhibited the increase of BLA, decreased the level of SUN and increased the hepatic and muscle glycogen content of mice. Thus, TFSL may have potential as an anti-fatigue agent.

  11. Toxic Ipomeamarone accumulation in healthy parts of Sweetpotato (Ipomoea batatas L. Lam) storage roots upon infection by Rhizopus stolonifer.

    Science.gov (United States)

    Wamalwa, Lydia N; Cheseto, Xavier; Ouna, Elizabeth; Kaplan, Fatma; Maniania, Nguya K; Machuka, Jesse; Torto, Baldwyn; Ghislain, Marc

    2015-01-14

    Furanoterpenoid accumulation in response to microbial attack in rotting sweetpotatoes has long been linked to deaths and lung edema of cattle in the world. However, it is not known whether furanoterpenoid ipomeamarone accumulates in the healthy-looking parts of infected sweetpotato storage roots. This is critical for effective utilization as animal feed and assessment of the potential negative impact on human health. Therefore, we first identified the fungus from infected sweetpotatoes as a Rhizopus stolonifer strain and then used it to infect healthy sweetpotato storage roots for characterization of furanoterpenoid content. Ipomeamarone and its precursor, dehydroipomeamarone, were identified through spectroscopic analyses, and detected in all samples and controls at varying concentrations. Ipomeamarone concentration was at toxic levels in healthy-looking parts of some samples. Our study provides fundamental information on furanoterpenoids in relation to high levels reported that could subsequently affect cattle on consumption and high ipomeamarone levels in healthy-looking parts.

  12. Toxic Ipomeamarone Accumulation in Healthy Parts of Sweetpotato (Ipomoea batatas L. Lam) Storage Roots upon Infection by Rhizopus stolonifer

    Science.gov (United States)

    2015-01-01

    Furanoterpenoid accumulation in response to microbial attack in rotting sweetpotatoes has long been linked to deaths and lung edema of cattle in the world. However, it is not known whether furanoterpenoid ipomeamarone accumulates in the healthy-looking parts of infected sweetpotato storage roots. This is critical for effective utilization as animal feed and assessment of the potential negative impact on human health. Therefore, we first identified the fungus from infected sweetpotatoes as a Rhizopus stolonifer strain and then used it to infect healthy sweetpotato storage roots for characterization of furanoterpenoid content. Ipomeamarone and its precursor, dehydroipomeamarone, were identified through spectroscopic analyses, and detected in all samples and controls at varying concentrations. Ipomeamarone concentration was at toxic levels in healthy-looking parts of some samples. Our study provides fundamental information on furanoterpenoids in relation to high levels reported that could subsequently affect cattle on consumption and high ipomeamarone levels in healthy-looking parts. PMID:25418792

  13. (Ipomoea batatas) clones of Togo

    African Journals Online (AJOL)

    Tozo koffi

    Sweet potato (Ipomoea batatas) is an hexaploide plant with complex incompatibility that reduces chances of self-fertilization. In vitro culture of sweet potato by means of biotechnological approaches is an advantage for its study and variety improvement. Three sweet potato cultivars from Togo: Damadoami, Tombolo ...

  14. Adaptation to mid-season drought in a sweetpotato (Ipomoea batatas [L.] Lam germplasm collection grown in Mozambique

    Directory of Open Access Journals (Sweden)

    Makunde Godwill S.

    2017-02-01

    Full Text Available Drought has negative effects on sweetpotato production. Two experiments with two watering treatments (irrigated and water-stressed were conducted at Umbeluzi Research Station in 2015. The objectives were to (i determine response of 48 sweetpotato germplasms to mid-season drought, (ii determine best traits for improvement of storage root yield under mid-season drought and (iii assess the selection criteria for identifying drought tolerance in sweetpotato germplasms. The irrigated and water- stressed trials received 640 and 400 mm of water, respectively, throughout the season. Water stress was imposed from 30 to 70 days after planting. Each treatment had two replicates arranged in a randomized complete block design. Data collected on storage root and vine yield and derived drought tolerance indices including harvest index were subjected to analysis of variance in R. Sweetpotato germplasms with high storage root yield under mid-season drought were associated with a high harvest index. Harvest index stability and the geometric mean are key to identifying cultivars with high and stable storage root yield under both treatments. MUSGP0646-126, Irene and Ivone combined both low TOL, SSI, HI and high yield storage root yield across the treatments and over seasons. The use of drought and harvest indices is encouraged for selecting improved cultivars for varied production environments and their regular use in accelerated breeding schemes is suggested.

  15. Evaluation of sweetpotato (Ipomoea batatas (L.) Lam.) germplasm from north-eastern Uganda through a Farmer Participatory Approach

    NARCIS (Netherlands)

    Abidin, P.E.; Eeuwijk, van F.A.; Stam, P.; Struik, P.C.; Zhang, D.P.; Hermann, M.; Carey, E.E.

    2002-01-01

    Ugandan farmers grow many landrace sweetpotato varieties, but some of these are relatively low yielding and susceptible to pests. The objective of the present research was to involve farmers in a large-scale assessment of Ugandan farmers’ varieties to rapidly identify those with superior yield

  16. Distributions, ex situ conservation priorities, and genetic resources potential of crop wild relatives of sweeetpotato (Ipomoea batatas (L.) Lam. I. series Batatas)

    Science.gov (United States)

    Crop wild relatives (CWR) of sweetpotato [Ipomoea batatas (L.) Lam., I. series Batatas] have the potential to contribute to breeding objectives for this important root crop. Uncertainty in regard to species boundaries and their phylogenetic relationships, and the limited availability of germplasm wi...

  17. Involvement of an ABI-like protein and a Ca2+-ATPase in drought tolerance as revealed by transcript profiling of a sweetpotato somatic hybrid and its parents Ipomoea batatas (L.) Lam. and I. triloba L.

    Science.gov (United States)

    Yang, Yufeng; Wang, Yannan; Jia, Licong; Yang, Guohong; Xu, Xinzhi; Zhai, Hong; He, Shaozhen; Li, Junxia; Dai, Xiaodong; Qin, Na; Zhu, Cancan; Liu, Qingchang

    2018-01-01

    Previously, we obtained the sweetpotato somatic hybrid KT1 from a cross between sweetpotato (Ipomoea batatas (L.) Lam.) cv. Kokei No. 14 and its drought-tolerant wild relative I. triloba L. KT1 not only inherited the thick storage root characteristic of Kokei No. 14 but also the drought-tolerance trait of I. triloba L. The aim of this study was to explore the molecular mechanism of the drought tolerance of KT1. Four-week-old in vitro-grown plants of KT1, Kokei No. 14, and I. triloba L. were subjected to a simulated drought stress treatment (30% PEG6000) for 0, 6, 12 and 24 h. Total RNA was extracted from samples at each time point, and then used for transcriptome sequencing. The gene transcript profiles of KT1 and its parents were compared to identify differentially expressed genes, and drought-related modules were screened by a weighted gene co-expression network analysis. The functions of ABI-like protein and Ca2+-ATPase, two proteins screened from the cyan and light yellow modules, were analyzed in terms of their potential roles in drought tolerance in KT1 and its parents. These analyses of the drought responses of KT1 and its somatic donors at the transcriptional level provide new annotations for the molecular mechanism of drought tolerance in the somatic hybrid KT1 and its parents.

  18. Compatibility and economic assessment of sweetpotato and garden ...

    African Journals Online (AJOL)

    ecological zone of Nigeria, to determine the compatibility and economic viability of sweetpotato (Ipomoea batatas) and garden egg (Solanum gelio) intercrop during 2011 and 2012 cropping seasons. Two sweetpotato varieties; NR05/022 and ...

  19. Green light synergistically enhances male sweetpotato weevil sex pheromone response

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lamarck, commercially grown in over 100 countries, is the 7th most important staple crop in the world. Sweetpotato weevil is a major pest of sweetpotato in most areas of cultivation, the feeding of which induces production in the sweetpotato root of extremely bitter...

  20. The anthocyanidin synthase gene from sweetpotato [Ipomoea ...

    African Journals Online (AJOL)

    USER

    2010-06-21

    Jun 21, 2010 ... 1Laboratory of Natural Products and Metabolic Engineering, Chongqing Sweetpotato Engineering Technological. Research Center ... 3Institute of Ornamental Plants Research, Chongqing Sweetpotato Engineering Technological Research Center, ... mainly women grow sweet potato (Luo et al., 2006). The.

  1. Eradication programs of two sweetpotato [Ipomoea batatas] pests, Cylas formicarius and Euscepes postfasciatus, in Japan with special reference to their dispersal ability

    International Nuclear Information System (INIS)

    Moriya, S.; Miyatake, T.

    2001-01-01

    Eradication programs are being implemented for 2 sweetpotato weevil pests, Cylas formicarius and Euscepes postfasciatus, in the Ryiikyu Islands located in the southernmost part of Japan by the application of the sterile insect technique (SIT). As it is essential for the implementation of the programs to assess the dispersal ability of the weevils, recent studies were reviewed. Both the flight and walking ability of C. formicarius was much higher in males than in females when determined in the laboratory. Synthesized sex pheromone of C. formicarius has been used as a strong lure to capture the males in the field. Results of the mark-recapture experiments suggest that a distance of at least 2 km is needed for the range of the buffer zone to separate the SIT target area from others. Since E. postfasciatus is unable to fly, only the walking ability was evaluated in the laboratory, indicating that the females exhibited a fairly higher locomotion activity compared with the males. Since no effective attractant for E. postfasciatus has been identified yet, information on the dispersal activity in the field is limited. Fundamental studies should be conducted to determine the dispersal ability of both weevil pests to implement successfully the eradication programs

  2. ‘Covington’ sweetpotato

    Science.gov (United States)

    ‘Covington’ is an orange-fleshed, smooth-skinned, rose-colored, table-stock sweetpotato [Ipomoea batatas (L.) Lam.] developed by North Carolina State University (NCSU). ‘Covington’, named after the late Henry M. Covington an esteemed sweetpotato scientist at NC State, was evaluated as NC98-608 in mu...

  3. In vitro regeneration of sweet potato (Ipomoea batatas (L.) Lam ...

    African Journals Online (AJOL)

    Ipomoea batatas (L.) Lam.) yield. The establishment of in vitro regeneration protocol for this plant is essential to improve it through tissue culture and genetic engineering. The objective of this study was to establish in vitro regeneration protocol ...

  4. Sweet potato for biomass. [Ipomoea batatas

    Energy Technology Data Exchange (ETDEWEB)

    Dangler, J.M.; Locascio, S.J.; Halsey, L.H.

    1984-01-01

    Field experiments were conducted in 1980 and 1981 to determine the root and plant top yield of sweet potato (Ipomoea batatas L. (Lam)) grown on a sandy soil. Cultivars 'GaTG-3', 'Morado', 'Rojo Blanco', 'Travis' and 'White Star' were evaluated at 2 harvest times. Mean starch yields from 'GaTG-3' at 105-15 days (7.2 t/ha) and at 210-30 days (9.6 t/ha) during two seasons were higher than from the other cultivars. With an increase in the growth period from 105-15 to 210-30 days the mean starch yield increased from 4.6 to 7.3 t/ha but the starch concentration of all cultivars decreased significantly during the same period. 17 references, 2 tables.

  5. Protective Effect of Purple Sweet Potato (Ipomoea batatas Linn ...

    African Journals Online (AJOL)

    Purpose: To evaluate the protective effects of purple sweet potato (Ipomoea batatas Linn, Convolvulaceae) extract (IBE) in stimulated BV-2 microglial cells and its anti-oxidant properties. Methods: Cell viability assessment was performed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) assay.

  6. Effects of feeding sweet potato ( Ipomoea batatas ) leaves on growth ...

    African Journals Online (AJOL)

    Forty-five (45) weaner rabbits were randomly allotted to five dietary treatments (T) to evaluate the effects of graded levels of sweet potato (Ipomoea batatas) leaves (SPL) and pelletized concentrate feed (PCF) on growth performance and nutrient digestibility of rabbits. The treatments were: T1 (0% SPL; 100% PCF); T2 (25% ...

  7. Growth and yield responses of sweet potato (ipomoea batatas) to ...

    African Journals Online (AJOL)

    Two field experiments were conducted at the Michael Okpara University of Agriculture, Umudike research farm in 2005 and 2006 wet seasons to determine the growth and yield responses of two sweet potato (Ipomoea batatas L. Lam) varieties viz TIS 87/0087 and TIS 8164 to time of potassium (K) fertilizer application in a ...

  8. Aqueous Extract of Ipomoea batatas Reduces Food Intake in Male ...

    African Journals Online (AJOL)

    help in weight management in the long run. Potato, a common starchy tuber in our environment is believed to contain substances that can help maintain and increases short‑term satiety. Aim: This study was conducted to determine the effect of the aqueous extract of sweet potato (Ipomoea batatas [IB]) on food intake in male ...

  9. Insect Interactions in Sweetpotato Breeding Nurseries

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lam. (Convolvulaceae), is a vital staple food crop in much of the developing world, and it is an important specialty crop in the United States. American consumers prefer sweetpotatoes with sweet, moist orange flesh. After many years of decline beginning in the 195...

  10. Management of sweet potato leaf curl virus in sweetpotatoes using insecticides

    Science.gov (United States)

    Sweetpotato leaf curl virus (SPLCV), which is transmitted by the sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), can severely affect yields of commercial sweetpotatoes, Ipomoea batatas (L.) Lam. (Convolvulaceae). This virus occurs every year at the U.S. Vegetable Laborato...

  11. Sweetpotato breeding for northeastern Uganda: farmer varieties, farmer-participatory selection, and stability of performance

    NARCIS (Netherlands)

    Abidin, P.E.

    2004-01-01

    Keywords: Agro-biodiversity, farmer varieties, indigenous knowledge, farmer-participatory research, genetic diversity, genotype-by-environment interaction, germplasm collection, Ipomoea batatas , specific adaptation, yield stability, sweetpotato, variance component

  12. Genetic analysis of yield and flesh colour in sweetpotato | Naidoo ...

    African Journals Online (AJOL)

    Pre-breeding information on the inheritance mechanism of important sweetpotato (Ipomoea batatas L.) agronomic traits is still limited. This study aimed at assessing the inheritance of five sweetpotato agronomic traits, viz. marketable fresh root yield (MFRY) and number (MNR), total fresh root yield (TFRY) and number (TNR) ...

  13. An Ipomoea batatas iron-sulfur cluster scaffold protein gene, IbNFU1, is involved in salt tolerance.

    Science.gov (United States)

    Liu, Degao; Wang, Lianjun; Liu, Chenglong; Song, Xuejin; He, Shaozhen; Zhai, Hong; Liu, Qingchang

    2014-01-01

    Iron-sulfur cluster biosynthesis involving the nitrogen fixation (Nif) proteins has been proposed as a general mechanism acting in various organisms. NifU-like protein may play an important role in protecting plants against abiotic and biotic stresses. An iron-sulfur cluster scaffold protein gene, IbNFU1, was isolated from a salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line LM79 in our previous study, but its role in sweetpotato stress tolerance was not investigated. In the present study, the IbNFU1 gene was introduced into a salt-sensitive sweetpotato cv. Lizixiang to characterize its function in salt tolerance. The IbNFU1-overexpressing sweetpotato plants exhibited significantly higher salt tolerance compared with the wild-type. Proline and reduced ascorbate content were significantly increased, whereas malonaldehyde (MDA) content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbNFU1 up-regulated pyrroline-5-carboxylate synthase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS) scavenging genes was found in the transgenic plants under salt stress. These findings suggest that IbNFU1gene is involved in sweetpotato salt tolerance and enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system.

  14. An Ipomoea batatas Iron-Sulfur Cluster Scaffold Protein Gene, IbNFU1, Is Involved in Salt Tolerance

    Science.gov (United States)

    Song, Xuejin; He, Shaozhen; Zhai, Hong; Liu, Qingchang

    2014-01-01

    Iron-sulfur cluster biosynthesis involving the nitrogen fixation (Nif) proteins has been proposed as a general mechanism acting in various organisms. NifU-like protein may play an important role in protecting plants against abiotic and biotic stresses. An iron-sulfur cluster scaffold protein gene, IbNFU1, was isolated from a salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line LM79 in our previous study, but its role in sweetpotato stress tolerance was not investigated. In the present study, the IbNFU1 gene was introduced into a salt-sensitive sweetpotato cv. Lizixiang to characterize its function in salt tolerance. The IbNFU1-overexpressing sweetpotato plants exhibited significantly higher salt tolerance compared with the wild-type. Proline and reduced ascorbate content were significantly increased, whereas malonaldehyde (MDA) content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbNFU1 up-regulated pyrroline-5-carboxylate synthase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS) scavenging genes was found in the transgenic plants under salt stress. These findings suggest that IbNFU1gene is involved in sweetpotato salt tolerance and enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system. PMID:24695556

  15. Persilangan Interspesifik Ipomoea Batatas (L.) Lam. Dengan Ipomoea Trifida (H.b.k.) G. Don. Berumbi Asal Citatah Jawa Barat

    OpenAIRE

    Setiawati, Tia; Karuniawan, Agung; Supriatun, Titin; Karyono, Karyono

    2016-01-01

    Ipomoea trifida merupakan kerabat liar ubi jalar (I. batatas) yang sangat berpotensi sebagai sumber gen dalam pemuliaan untuk memperbaiki karakter daya hasil, kadar bahan kering, pati, protein, ketahanan terhadap hama, dan penyakit tertentu. Persilangan interspesifik merupakan salah satu upaya untuk meningkatkan keragaman genetik dalam program pemuliaan tanaman, sehingga kajian mengenai persilangan interspesifik antara I. batatas dengan I. trifida menjadi sangat penting dilakukan. Tujuan pene...

  16. Trapping sweetpotato weevil, Cylas formicarius elegantulus (Coleoptera: Brentidae), with high doses of sex pheromone: Catch enhancement and weathering rate in Hawaii

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lamarck, one of the top ten staple crops produced worldwide, has increased in production in Hawaii in recent years. The sweetpotato weevil, Cylas formicarius elegantulus (Summers)(Coleoptera: Brentidae), is a major economic and quarantine pest of sweetpotato in Hawa...

  17. Natural antifungal compounds from the peels of Ipomoea batatas Lam.

    Science.gov (United States)

    Oluyori, Abimbola P; Shaw, Arun Kumar; Preeti, Rastogi; Reddy, Sammajay; Atolani, Olubunmi; Olatunji, Gabriel A; Fabiyi, Oluwatoyin A

    2016-09-01

    Three antifungal compounds have been isolated for the first time from the peels of Ipomoea batatas Lam. Their structures were established on the basis of 1D and 2D NMR spectra data as well as ESI-MS and IR analysis. Urs-13(18)-ene-3β-yl acetate was found to possess a weak activity against Sporothrix schenckii and Trichophyton metagrophytes fungi with an MIC value of 50 μg/mL each. Stigmasterol and 3-friedelanol were equally active against T. metagrophytes.

  18. Wound healing activity of Ipomoea batatas tubers (sweet potato

    Directory of Open Access Journals (Sweden)

    Madhav Sonkamble

    2011-10-01

    Full Text Available Background: Ipomoea batatas (L. Lam. from the family Convolvulaceae is the world’s sixth largest food crop. The tubers of Ipomoea batatas commonly known as sweet potato are consumed as a vegetable globally. The tubers contain high levels of polyphenols such as anthocyanins and phenolic acids and vitamins A, B and C, which impart a potent antioxidant activity that can translate well to show wound healing effects. To check their effects on wound healing, the peels and peel bandage were tested on various injury models in rats in the present study.Methods: The methanolic extracts of the peels and peel bandage of Ipomoea batatas tubers (sweet potato were screened for wound healing by excision and incision wound models on Wistar rats. Three types of gel formulations were prepared, viz., gel containing 3.0% (w/w peel extract, gel containing 6.0% (w/w peel extract and gel containing 10% (w/w peel extract. Betadine (5% w/w povidone iodine cream was used as a reference standard. In the incision wound model, Tensile strength of the skin was measured. Epithelization time, wound contraction, hydroxyproline content of the scab, and ascorbic acid and malondialdehyde content of the plasma were determined in the excision wound model.Results: In the incision wound model, high tensile strength of the wounded skin was observed in animals treated with the peel extract gels and the peel bandage when compared with wounded control animals. The increase in tensile strength indicates the promotion of collagen fibers and that the disrupted wound surfaces are being firmly knit by collagen. In the excision wound model, significant wound closure was observed on the 4th day in rats treated with all three gel formulations when compared with the wounded control rats. A significant increase inFunctional Foods in Health and Disease 2011; 10:403-415hydroxyproline and ascorbic acid content in the gel-treated animals and a significant decrease in malondialdehyde content in the

  19. ‘Charleston Scarlet’ Sweetpotato

    Science.gov (United States)

    The sweetpotato [Ipomoea batatas (L.) Lam.] cultivar, ‘Charleston Scarlet’ was developed by the U.S. Department of Agriculture, Agricultural Research Service, Charleston, SC. ‘Charleston Scarlet’ produces orange-fleshed, sweet storage roots with attractive scarlet-colored skin (periderm). Vine gro...

  20. [Study on antitumor effect and its toxicity of Ipomoea Batatas Poir Cv anthocyanins].

    Science.gov (United States)

    Liu, Ning; Wang, Hongbing; Wang, Chunbo

    2008-07-01

    To investigate the antitumor effect and estimate the toxicity of Ipomoea Batatas Poir Cv anthocyanins. Mice sarcinoma S180 and mice liver cancer H22 were administered with positive Ftorafur and different dose of Ipomoea Batatas Poir Cv anthocyanins by pouring into the stomach. The antitumor effects of Ipomoea Batatas Poir Cv anthocyanins were observed by calculating the inhibition rate. Subchronic toxicity tests and micronucleus test of bone marrow cell in mice and Ames test were carried out to evaluate the toxicity. At the doses of 150 mg and 75 mg Ipomoea Batatas Poir Cv anthocyanins, the rates of sarcinoma 180 inhibition were 45.04% and 36.64% respectively. The rate of liver cancer H22 inhibition at the dose of 150mg Batatas Poir Cv anthocyanins was 33.33% . The result of subchronic toxicity tests showed that Ipomoea Batatas Poir Cv anthocyanins had no obvious toxicity to rats. The results of the Ames test and micronucleus test were negative. Ipomoea Batatas Poir Cv anthocyanins could have inhibitory effect on transplantation tumor of mice, and have no toxicity and no mutation.

  1. Processos biocatalÃticos utilizando o complexo enzimÃtico dos rizomas de Ipomoea batatas (batata-doce)

    OpenAIRE

    Leonardo AlcÃntara Alves

    2013-01-01

    A espÃcie Ipomoea batatas, pertencente à famÃlia Convolvulaceae e popularmente conhecida como batata-doce, batata, camote, boniato, apichu e kumara à uma planta tuberosa de folhas longas e caule que atinge atà 3 metros de comprimento sendo cultivada em todo Brasil devido a sua ampla capacidade de adaptaÃÃo. Diversos trabalhos encontrados na literatura envolvendo o isolamento de substÃncias da batata-doce (BD) descreveram a presenÃa de compostos com atividades biolÃgicas e farmacolÃgicas em su...

  2. Ipomoea batatas and Agarics blazei ameliorate diabetic disorders with therapeutic antioxidant potential in streptozotocin-induced diabetic rats

    OpenAIRE

    Niwa, Atsuko; Tajiri, Takashi; Higashino, Hideaki

    2011-01-01

    Ipomoea batatas, Agaricus blazei and Smallanthus sonchifolius are known to favorably influence diabetes mellitus. To clarify their antidiabetic efficacy and hypoglycemic mechanisms, we treated streptozotocin-induced diabetic rats with daily oral feeding of powdered Ipomoea batatas (5 g kg−1 d−1), Agaricus blazei (1 g kg−1 d−1) or Smallanthus sonchifolius (4 g kg−1 d−1) for 2 months. Treatments with Ipomoea batatas or Agaricus blazei, but not Smallanthus sonchifolius, significantly suppressed ...

  3. The resin glycosides from the sweet potato (Ipomoea batatas L. LAM.).

    Science.gov (United States)

    Noda, Naoki; Horiuchi, Yoshinori

    2008-11-01

    Four new and two known ether-soluble resin glycosides were isolated from popular sweet potato (the roots of Ipomoea batatas L. LAM., Kokei 14 go, Convolvulaceae) in Japan. Unlike ester-type dimers, batatins I and II, obtained from other sweet potato (Ipomoea batabas var. batatas), the glycosides were tetra or pentasaccharide monomers in which the sugar moieties are partially acylated by organic acids and combine with the aglycone, jalapinolic acid, to form a macrocyclic ester.

  4. Sensorial, physical and chemical evaluation of bio-fortified Ipomoea batatas

    OpenAIRE

    Mara N.G. Santos; Joice V.C. Orsine; Alexandre I. de A. Pereira; Roberto Cañete; María R.C.G. Novaes

    2014-01-01

    Context: Bio-fortified sweet potato, Ipomoea batatas (L.) Lamarck beta-carotene rich, has been included in the most vulnerable population’s diet with the purpose of reducing health disturbances associated with hipovitaminosis. Aims:To evaluate a physical and chemical analysis of total carotenes and beta-carotene and to determine the antioxidant potential as well as to carry out a sensorial analysis of the bio-fortified Ipomoea batatas (L.) Lamarck (BDB) in nature and processed. Method...

  5. Transcriptional profiling of sweetpotato (Ipomoea batatas) roots indicates down-regulation of lignin biosynthesis and up-regulation of starch biosynthesis at an early stage of storage root formation.

    Science.gov (United States)

    Firon, Nurit; LaBonte, Don; Villordon, Arthur; Kfir, Yanir; Solis, Julio; Lapis, Evgenia; Perlman, Temima Schnitzer; Doron-Faigenboim, Adi; Hetzroni, Amots; Althan, Leviah; Adani Nadir, Lahan

    2013-07-09

    The number of fibrous roots that develop into storage roots determines sweetpotato yield. The aim of the present study was to identify the molecular mechanisms involved in the initiation of storage root formation, by performing a detailed transcriptomic analysis of initiating storage roots using next-generation sequencing platforms. A two-step approach was undertaken: (1) generating a database for the sweetpotato root transcriptome using 454-Roche sequencing of a cDNA library created from pooled samples of two root types: fibrous and initiating storage roots; (2) comparing the expression profiles of initiating storage roots and fibrous roots, using the Illumina Genome Analyzer to sequence cDNA libraries of the two root types and map the data onto the root transcriptome database. Use of the 454-Roche platform generated a total of 524,607 reads, 85.6% of which were clustered into 55,296 contigs that matched 40,278 known genes. The reads, generated by the Illumina Genome Analyzer, were found to map to 31,284 contigs out of the 55,296 contigs serving as the database. A total of 8,353 contigs were found to exhibit differential expression between the two root types (at least 2.5-fold change). The Illumina-based differential expression results were validated for nine putative genes using quantitative real-time PCR. The differential expression profiles indicated down-regulation of classical root functions, such as transport, as well as down-regulation of lignin biosynthesis in initiating storage roots, and up-regulation of carbohydrate metabolism and starch biosynthesis. In addition, data indicated delicate control of regulators of meristematic tissue identity and maintenance, associated with the initiation of storage root formation. This study adds a valuable resource of sweetpotato root transcript sequences to available data, facilitating the identification of genes of interest. This resource enabled us to identify genes that are involved in the earliest stage of storage

  6. Transcriptional profiling of sweetpotato (Ipomoea batatas) roots indicates down-regulation of lignin biosynthesis and up-regulation of starch biosynthesis at an early stage of storage root formation

    Science.gov (United States)

    2013-01-01

    Background The number of fibrous roots that develop into storage roots determines sweetpotato yield. The aim of the present study was to identify the molecular mechanisms involved in the initiation of storage root formation, by performing a detailed transcriptomic analysis of initiating storage roots using next-generation sequencing platforms. A two-step approach was undertaken: (1) generating a database for the sweetpotato root transcriptome using 454-Roche sequencing of a cDNA library created from pooled samples of two root types: fibrous and initiating storage roots; (2) comparing the expression profiles of initiating storage roots and fibrous roots, using the Illumina Genome Analyzer to sequence cDNA libraries of the two root types and map the data onto the root transcriptome database. Results Use of the 454-Roche platform generated a total of 524,607 reads, 85.6% of which were clustered into 55,296 contigs that matched 40,278 known genes. The reads, generated by the Illumina Genome Analyzer, were found to map to 31,284 contigs out of the 55,296 contigs serving as the database. A total of 8,353 contigs were found to exhibit differential expression between the two root types (at least 2.5-fold change). The Illumina-based differential expression results were validated for nine putative genes using quantitative real-time PCR. The differential expression profiles indicated down-regulation of classical root functions, such as transport, as well as down-regulation of lignin biosynthesis in initiating storage roots, and up-regulation of carbohydrate metabolism and starch biosynthesis. In addition, data indicated delicate control of regulators of meristematic tissue identity and maintenance, associated with the initiation of storage root formation. Conclusions This study adds a valuable resource of sweetpotato root transcript sequences to available data, facilitating the identification of genes of interest. This resource enabled us to identify genes that are involved

  7. Combining ability of sweetpotato germplasm for yield, dry matter content, and anthocyanin production

    Science.gov (United States)

    Interest in the potential of sweetpotato (Ipomoea batatas) for the production of industrial products is increasing. As part of an effort to evaluate the potential of sweetpotatoes for starch and anthocyanin production in the southeastern United States, a 5 x 5 North Carolina mating design II (NCII m...

  8. Product evaluation for reniform nematode suppression in Mississippi Delta sweetpotato production, 2011

    Science.gov (United States)

    The reniform nematode, Rotylenchulus reniformis, can cause significant losses in sweetpotato, Ipomoea batatas, production in the Mississippi Delta. Reniform nematode is a microscopic plant parasite that feeds on sweetpotato roots causing severe stunting of root growth. Reduction in yield due to the ...

  9. Release of two orange-fleshed sweetpotato cultivars, 'SPK004' ('Kakamega') and 'Ejumula', in Uganda

    NARCIS (Netherlands)

    Mwanga, R.O.M.; Odongo, B.; Niringiye, C.; Alajo, A.; Abidin, P.E.; Kapinga, R.; Tumwegamire, S.; Lemaga, B.; Nsumba, J.; Carey, E.E.

    2007-01-01

    Two orange-fleshed landrace sweetpotato [Ipomoea batatas L. (Lam.)] cultivars named ‘SPK004’ (‘Kakamega’) and ‘Ejumula’ were approved for release by the Ugandan Plant Variety Release Committee in Apr. 2004 (Mwanga et al., 2004a). This is the third lot of sweetpotato cultivars to be officially

  10. Current status and future direction of sweetpotato research at the U. S. Vegetable Laboratory

    Science.gov (United States)

    Sweetpotato (Ipomoea batatas L.) is one of the world’s most important root crops that is grown in more than 100 countries worldwide. It is an important specialty crop in the U.S. Since 1939, there has been continuous research on sweetpotato at the USDA, and for over 40 years at the U.S. Vegetable ...

  11. Ipomoea batatas (L.) Lam.: a rich source of lipophilic phytochemicals.

    Science.gov (United States)

    Cordeiro, Nereida; Freitas, Nélia; Faria, Marisa; Gouveia, Manuela

    2013-12-18

    The lipophilic extracts from the storage root of 13 cultivars of sweet potato (Ipomoea batatas (L.) Lam.) were evaluated by gas chromatography-mass spectrometry with the aim to valorize them and offer information on their nutritional properties and potential health benefits. The amount of lipophilic extractives ranged from 0.87 to 1.32% dry weight. Fatty acids and sterols were the major families of compounds identified. The most abundant saturated and unsaturated fatty acids were hexadecanoic acid (182-428 mg/kg) and octadeca-9,12-dienoic acid (133-554 mg/kg), respectively. β-Sitosterol was the principal phytosterol, representing 55.2-77.6% of this family, followed by campesterol. Long-chain aliphatic alcohols and α-tocopherol were also detected but in smaller amounts. The results suggest that sweet potato should be considered as an important dietary source of lipophilic phytochemicals.

  12. Ipomoea batatas and Agarics blazei ameliorate diabetic disorders with therapeutic antioxidant potential in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Niwa, Atsuko; Tajiri, Takashi; Higashino, Hideaki

    2011-05-01

    Ipomoea batatas, Agaricus blazei and Smallanthus sonchifolius are known to favorably influence diabetes mellitus. To clarify their antidiabetic efficacy and hypoglycemic mechanisms, we treated streptozotocin-induced diabetic rats with daily oral feeding of powdered Ipomoea batatas (5 g kg(-1) d(-1)), Agaricus blazei (1 g kg(-1) d(-1)) or Smallanthus sonchifolius (4 g kg(-1) d(-1)) for 2 months. Treatments with Ipomoea batatas or Agaricus blazei, but not Smallanthus sonchifolius, significantly suppressed the increases of fasting plasma glucose and hemoglobin A1c levels, and restored body weight loss during diabetes. Serum insulin levels after oral glucose administration tests increased along the treatments of Ipomoea batatas or Agaricus blazei. Moreover, Ipomoea batatas and Agaricus blazei reduced superoxide production from leukocytes and vascular homogenates, serum 8-oxo-2'-deoxyguanosine, and vascular nitrotyrosine formation of diabetic rats to comparable levels of normal control animals. Stress- and inflammation-related p38 mitogen-activated protein kinase activity and tumor necrosis factor-α production of diabetic rats were significantly depressed by Ipomoea batatas administration. Histological examination also exhibited improvement of pancreatic β-cells mass after treatments with Ipomoea batatas or Agaricus blazei. These results suggest that hypoglycemic effects of Ipomoea batatas or Agaricus blazei result from their suppression of oxidative stress and proinflammatory cytokine production followed by improvement of pancreatic β-cells mass.

  13. Ipomoea batatas and Agarics blazei ameliorate diabetic disorders with therapeutic antioxidant potential in streptozotocin-induced diabetic rats

    Science.gov (United States)

    Niwa, Atsuko; Tajiri, Takashi; Higashino, Hideaki

    2011-01-01

    Ipomoea batatas, Agaricus blazei and Smallanthus sonchifolius are known to favorably influence diabetes mellitus. To clarify their antidiabetic efficacy and hypoglycemic mechanisms, we treated streptozotocin-induced diabetic rats with daily oral feeding of powdered Ipomoea batatas (5 g kg−1 d−1), Agaricus blazei (1 g kg−1 d−1) or Smallanthus sonchifolius (4 g kg−1 d−1) for 2 months. Treatments with Ipomoea batatas or Agaricus blazei, but not Smallanthus sonchifolius, significantly suppressed the increases of fasting plasma glucose and hemoglobin A1c levels, and restored body weight loss during diabetes. Serum insulin levels after oral glucose administration tests increased along the treatments of Ipomoea batatas or Agaricus blazei. Moreover, Ipomoea batatas and Agaricus blazei reduced superoxide production from leukocytes and vascular homogenates, serum 8-oxo-2'-deoxyguanosine, and vascular nitrotyrosine formation of diabetic rats to comparable levels of normal control animals. Stress- and inflammation-related p38 mitogen-activated protein kinase activity and tumor necrosis factor-α production of diabetic rats were significantly depressed by Ipomoea batatas administration. Histological examination also exhibited improvement of pancreatic β-cells mass after treatments with Ipomoea batatas or Agaricus blazei. These results suggest that hypoglycemic effects of Ipomoea batatas or Agaricus blazei result from their suppression of oxidative stress and proinflammatory cytokine production followed by improvement of pancreatic β-cells mass. PMID:21562638

  14. ‘Liberty’ Dry-Fleshed Sweetpotato

    Science.gov (United States)

    The sweetpotato [Ipomoea batatas (L.) Lam.] cultivar, ‘Liberty’ was jointly developed by the U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS), and Clemson University, South Carolina Agriculture and Forestry Research System. This cultivar is a dry-fleshed type with attracti...

  15. Color analysis of storage roots from the USDA, ARS sweetpotato germplasm collection

    Science.gov (United States)

    USDA, ARS, Plant Genetic Resources Conservation Unit (PGRCU) in Griffin, GA, maintains the U.S. germplasm collection for Ipomoea spp. (Convolvulaceae). During 2012-2014, 737 sweetpotato, Ipomoea batatas (L.) Lam., plant introduction (PI) accessions were acquired as tissue culture plantlets, acclima...

  16. Identification of quantitative trait Loci for dry-matter, starch, and ß-carotene content in Sweetpotato

    Science.gov (United States)

    Development of orange-fleshed sweetpotatoes (OFSP) is essential for the improvement of the food supply and nutritional status of millions of people in developing countries, particularly in sub Saharan Africa. However, sweetpotato [Ipomoea batatas (L.) Lam] breeding is challenging due to its genetic ...

  17. Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati) under Salinity and Its Comparative Analysis with Sweetpotato

    Science.gov (United States)

    Solis, Julio; Baisakh, Niranjan; Brandt, Steven R.; Villordon, Arthur; La Bonte, Don

    2016-01-01

    The response and adaption to salt remains poorly understood for beach morning glory [Ipomoea imperati (Vahl) Griseb], one of a few relatives of sweetpotato, known to thrive under salty and extreme drought conditions. In order to understand the genetic mechanisms underlying salt tolerance of a Convolvulaceae member, a genome-wide transcriptome study was carried out in beach morning glory by 454 pyrosequencing. A total of 286,584 filtered reads from both salt stressed and unstressed (control) root and shoot tissues were assembled into 95,790 unigenes with an average length of 667 base pairs (bp) and N50 of 706 bp. Putative differentially expressed genes (DEGs) were identified as transcripts overrepresented under salt stressed tissues compared to the control, and were placed into metabolic pathways. Most of these DEGs were involved in stress response, membrane transport, signal transduction, transcription activity and other cellular and molecular processes. We further analyzed the gene expression of 14 candidate genes of interest for salt tolerance through quantitative reverse transcription PCR (qRT-PCR) and confirmed their differential expression under salt stress in both beach morning glory and sweetpotato. The results comparing transcripts of I. imperati against the transcriptome of other Ipomoea species, including sweetpotato are also presented in this study. In addition, 6,233 SSR markers were identified, and an in silico analysis predicted that 434 primer pairs out of 4,897 target an identifiable homologous sequence in other Ipomoea transcriptomes, including sweetpotato. The data generated in this study will help in understanding the basics of salt tolerance of beach morning glory and the SSR resources generated will be useful for comparative genomics studies and further enhance the path to the marker-assisted breeding of sweetpotato for salt tolerance. PMID:26848754

  18. Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati under Salinity and Its Comparative Analysis with Sweetpotato.

    Directory of Open Access Journals (Sweden)

    Julio Solis

    Full Text Available The response and adaption to salt remains poorly understood for beach morning glory [Ipomoea imperati (Vahl Griseb], one of a few relatives of sweetpotato, known to thrive under salty and extreme drought conditions. In order to understand the genetic mechanisms underlying salt tolerance of a Convolvulaceae member, a genome-wide transcriptome study was carried out in beach morning glory by 454 pyrosequencing. A total of 286,584 filtered reads from both salt stressed and unstressed (control root and shoot tissues were assembled into 95,790 unigenes with an average length of 667 base pairs (bp and N50 of 706 bp. Putative differentially expressed genes (DEGs were identified as transcripts overrepresented under salt stressed tissues compared to the control, and were placed into metabolic pathways. Most of these DEGs were involved in stress response, membrane transport, signal transduction, transcription activity and other cellular and molecular processes. We further analyzed the gene expression of 14 candidate genes of interest for salt tolerance through quantitative reverse transcription PCR (qRT-PCR and confirmed their differential expression under salt stress in both beach morning glory and sweetpotato. The results comparing transcripts of I. imperati against the transcriptome of other Ipomoea species, including sweetpotato are also presented in this study. In addition, 6,233 SSR markers were identified, and an in silico analysis predicted that 434 primer pairs out of 4,897 target an identifiable homologous sequence in other Ipomoea transcriptomes, including sweetpotato. The data generated in this study will help in understanding the basics of salt tolerance of beach morning glory and the SSR resources generated will be useful for comparative genomics studies and further enhance the path to the marker-assisted breeding of sweetpotato for salt tolerance.

  19. Uptake of iodine-131 in tropical crops. [Ipomoea batatas; Ipomoea reptans; Lycopersicon

    Energy Technology Data Exchange (ETDEWEB)

    Asprer, G.A.; Lansangan, L.M.

    1986-01-01

    Vegetable crops which include sweet potato tops (Ipomoea batatas), kangkong (Ipomoea repitans) and tomato plants were grown in dark-painted jars containing Hoagland-Arnon modified nutrient solution, utilizing the technique of hydroponics. The experiments for sweet potato tops and kangkong plants were duplicated for replicate studies and steady-state conditions were simulated throughout. Tomato plants were grown in the same manner but growth was observed to be hampered when starting from mature plants. Radioiodine was added to the nutrient medium containing 0.5% non-radioactive NaI solution. The solution in the jar was adjusted daily so as to maintain a constant concentration which would simulate routine releases that are essentially continuous. After incorporating the radioiodine to the solution, 10 ml aliquot was taken and counted for radioactivity by means of a 5'' x 5'' NaI(T1) detector connected to the multichannel gamma analyzer. Both plants and solution were counted for radioactivity at different time intervals using the same geometry. Results indicate that the activity in the plants were relatively higher than that of the solution. The activity tends to level off or decrease after a few days. The concentration factor which is the ratio of the activity in the plant (uCi/gm) over the activity in the medium (uCi/ml) varied for each time interval. 12 references, 2 figures, 3 tables.

  20. Susceptibility of sweetpotato (Ipomoea batatas) peel proteins to digestive enzymes

    Science.gov (United States)

    Sweet potato proteins have been shown to possess antioxidant and antidiabetic properties in vivo. The ability of a protein to exhibit systemic effects is somewhat unusual as proteins are typically susceptible to digestive enzymes. This study was undertaken to better understand how digestive enzymes ...

  1. Ipomoea batatas attenuates amyloid β peptide-induced neurotoxicity in ICR mice.

    Science.gov (United States)

    Kim, Jae Kyeom; Choi, Soo Jung; Cho, Hong Yon; Kim, Young Jun; Lim, Seung-Taik; Kim, Chang-Ju; Kim, Eun Ki; Kim, Hye Kyung; Peterson, Sabrina; Shin, Dong-Hoon

    2011-03-01

    In this study, the protective effects of 17 Korean native plants against amyloid β peptide (Aβ)-induced oxidative stress were screened using the 2',7'-dichlorofluorescin diacetate assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Ipomoea batatas exerted the highest protective effects against oxidative stress and was selected for further investigation. To confirm the protective activity of this extract, the I. batatas extract was fed to ICR mice that had been injected with Aβ to induce neuronal deficits. In these experiments, the extract of I. batatas significantly reversed Aβ-induced neurotoxicity as assessed by the passive avoidance test, a behavioral experiment. Moreover, I. batatas administration reduced the level of lipid peroxidation and increased catalase activities in biochemical studies using the brain tissue of mice. These results indicate that I. batatas might be beneficial against Alzheimer's disease, especially by limiting oxidative stress in the brain.

  2. [FISH analysis of chromosomes of sweet potato(Ipomoea batatas cv.Xushu No.18)].

    Science.gov (United States)

    Tang, Jia-Li; Qi, Da-Shi; Zhang, Yu; Liu, Hui-Juan; Sun, Jian-Ying; Cao, Qing-He; Ma, Dai-Fu; Li, Zong-Yun

    2010-02-01

    In order to understand the chromosome structure of sweet potato (Ipomoea batatas cv. Xushu 18), molecular cytogenetic analyses were carried out on I. batatas. by using 45S rDNA fluorescence in situ hybridization (45S rDNA-FISH), self genomic in situ hybridization (self-GISH), and silver staining techniques. Twelve, sixteen, and eighteen regions were silver stained in the interphase nucleus of I. batatas. The results of FISH analysis demonstrated 16 or 18 signals with different intensity on chromosomes of I. batatas. Self-GISH analysis showed that the intensive signals on I. batatas mitotic chromosomes were distributed along the chromosomes. However, the signals located in centromeric, subcentromeric, and telomeric regions were stronger and denser than those in other regions.

  3. Four new resin glycosides, murasakimasarins I-IV, from the tuber of Ipomoea batatas.

    Science.gov (United States)

    Ono, Masateru; Teramoto, Sena; Naito, Saori; Takahashi, Asuka; Yoneda, Akito; Shinkai, Masato; Taga, Naoki; Yasuda, Sin; Tsuchihasi, Ryota; Okawa, Masafumi; Kinjo, Junei; Yoshimitsu, Hitoshi; Nohara, Toshihiro

    2018-03-05

    Four new resin glycosides having macrolactone structures (jalapins), murasakimasarins I-IV, were isolated from the tubers of Ipomoea batatas (L.) Lam. ('Murasakimasari', Convolvulaceae), along with three known glycosides. Their structures were determined on the basis of spectroscopic data as well as chemical evidence. Murasakimasarin III is the first representative of a resin glycoside with 10-methylundecanoic acid as the component organic acid.

  4. Gamma irradiation effect on the chemical composition and the antioxidant activity of Ipomoea batatas L

    International Nuclear Information System (INIS)

    Tahir, D.; Halide, H.; Kurniawan, D.; Wahab, A. W.

    2014-01-01

    The chemical composition and antioxidant activity of Ipomoea batatas L. (sweet potato) were studied by x-ray fluorescence (XRF) spectroscopy, Fourier transform infrared spectroscopy, and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The irradiation treatment was performed by using Cs-137 as a gamma sources in experimental equipment. Treatment by irradiation emerges as a possible conservation technique that has been tested successfully in several food products. The amount of chemical composition was changed and resulting new chemical for absorbed dose 40 mSv. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by DPPH radical scavenging capacity. The antioxidant activity of Ipomoea batatas L. extract was dramatically increased in the non-irradiated sample to the sample irradiated at 40 mSv. These results indicate that gamma irradiation of Ipomoea batatas L. extract can enhance its antioxidant activity through the formation of a new chemical compound. Based on these results, increased antioxidant activity of Ipomoea batatas L. extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals

  5. Bio-deterioration of sweet potato (ipomoea batatas lam) in storage ...

    African Journals Online (AJOL)

    Michael Horsfall

    ABSTRACT: The biodeterioration of sweet potato (Ipomoea batatas) was investigated at Port. Harcourt, south southern Nigeria. Aspergillus niger, Fusarium oxysporum, Rhizopus stolonifer,. Botryodiplodia theobroma and Penicillium sp. were found to be associated with deteriorating sweet potato tubers and used for ...

  6. Gamma irradiation effect on the chemical composition and the antioxidant activity of Ipomoea batatas L.

    Science.gov (United States)

    Tahir, D.; Halide, H.; Wahab, A. W.; Kurniawan, D.

    2014-09-01

    The chemical composition and antioxidant activity of Ipomoea batatas L. (sweet potato) were studied by x-ray fluorescence (XRF) spectroscopy, Fourier transform infrared spectroscopy, and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The irradiation treatment was performed by using Cs-137 as a gamma sources in experimental equipment. Treatment by irradiation emerges as a possible conservation technique that has been tested successfully in several food products. The amount of chemical composition was changed and resulting new chemical for absorbed dose 40 mSv. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by DPPH radical scavenging capacity. The antioxidant activity of Ipomoea batatas L. extract was dramatically increased in the non-irradiated sample to the sample irradiated at 40 mSv. These results indicate that gamma irradiation of Ipomoea batatas L. extract can enhance its antioxidant activity through the formation of a new chemical compound. Based on these results, increased antioxidant activity of Ipomoea batatas L. extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  7. Gamma irradiation effect on the chemical composition and the antioxidant activity of Ipomoea batatas L

    Energy Technology Data Exchange (ETDEWEB)

    Tahir, D., E-mail: dtahir@fmipa.unhas.ac.id; Halide, H., E-mail: dtahir@fmipa.unhas.ac.id; Kurniawan, D. [Department of Physics, Hasanuddin University, Makassar 90245 (Indonesia); Wahab, A. W. [Department of Chemistry, Hasanuddin University, Makassar 90245 (Indonesia)

    2014-09-25

    The chemical composition and antioxidant activity of Ipomoea batatas L. (sweet potato) were studied by x-ray fluorescence (XRF) spectroscopy, Fourier transform infrared spectroscopy, and by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity. The irradiation treatment was performed by using Cs-137 as a gamma sources in experimental equipment. Treatment by irradiation emerges as a possible conservation technique that has been tested successfully in several food products. The amount of chemical composition was changed and resulting new chemical for absorbed dose 40 mSv. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by DPPH radical scavenging capacity. The antioxidant activity of Ipomoea batatas L. extract was dramatically increased in the non-irradiated sample to the sample irradiated at 40 mSv. These results indicate that gamma irradiation of Ipomoea batatas L. extract can enhance its antioxidant activity through the formation of a new chemical compound. Based on these results, increased antioxidant activity of Ipomoea batatas L. extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  8. Bio-deterioration of sweet potato ( ipomoea batatas lam ) in storage ...

    African Journals Online (AJOL)

    The biodeterioration of sweet potato (Ipomoea batatas) was investigated at Port Harcourt, south southern Nigeria. Aspergillus niger, Fusarium oxysporum, Rhizopus stolonifer, Botryodiplodia theobroma and Penicillium sp. were found to be associated with deteriorating sweet potato tubers and used for inoculation of fresh ...

  9. Characterization and expression of Rubisco activase genes in Ipomoea batatas.

    Science.gov (United States)

    Jiang, Yusong; Wang, Jianxi; Tao, Xiang; Zhang, Yizheng

    2013-11-01

    Two-dimensional electrophoresis, coupled with MALDI-TOF-MS, was used to identify differentially expressed proteins between young and mature leaves of sweet potato [Ipomoea batatas (L.) Lam]. The results showed that there were 25 differential proteins between young and mature leaves. The Rubisco activase (RCA) that catalyzes the activation of Rubisco in vivo and plays a crucial role in photosynthesis was among these 25 proteins. So far, little was known about the molecular biology of RCA in sweet potato. Here, this research reports the cloning and characterization of two genes encoding the short isoform and the long isoform of sweet potato RCAs. Analysis of DNA sequences of RCA suggested that the corresponding mRNAs were transcribed from two different genes. To study the roles of these two RCA isoforms in photosynthesis, we investigated the expression patterns of these RCA genes at the mRNA and protein levels every 2 h in a photoperiod and under different temperatures conditions. The results indicated that these two RCA isoforms may play different roles in regulating photosynthesis and they may be regulated by light, heat or both. In addition, there were interactions between Rubisco large subunit (RBCl) and short isoform RCA (RCAs) as well as RCAs and long isoform RCA (RCAl), but no interaction between RBCl and RCAl, implying they might form a sandwich-like structure (RBCl-RCAs-RCAl), at least in yeast cells. These results provided new information on the modulation of RCA genes in sweet potato, which could be useful in improving photosynthesis and plant growth in sweet potato.

  10. Novel begomovirus species of recombinant nature in sweet potato (Ipomoea batatas) and Ipomoea indica: taxonomic and phylogenetic implications.

    Science.gov (United States)

    Lozano, Gloria; Trenado, Helena P; Valverde, Rodrigo A; Navas-Castillo, Jesús

    2009-10-01

    Viral diseases occur wherever sweet potato (Ipomoea batatas) is cultivated and because this crop is vegetatively propagated, accumulation and perpetuation of viruses can become a major constraint for production. Up to 90% reductions in yield have been reported in association with viral infections. About 20 officially accepted or tentative virus species have been found in sweet potato and other Ipomoea species. They include three species of begomoviruses (genus Begomovirus, family Geminiviridae) whose genomes have been fully sequenced. In this investigation, we conducted a search for begomoviruses infecting sweet potato and Ipomoea indica in Spain and characterized the complete genome of 15 isolates. In addition to sweet potato leaf curl virus (SPLCV) and Ipomoea yellowing vein virus, we identified three new begomovirus species and a novel strain of SPLCV. Our analysis also demonstrated that extensive recombination events have shaped the populations of Ipomoea-infecting begomoviruses in Spain. The increased complexity of the unique Ipomoea-infecting begomovirus group, highlighted by our results, open new horizons to understand the phylogeny and evolution of the family Geminiviridae.

  11. Disentangling the origins of cultivated sweet potato (Ipomoea batatas (L.) Lam.).

    Science.gov (United States)

    Roullier, Caroline; Duputié, Anne; Wennekes, Paul; Benoit, Laure; Fernández Bringas, Víctor Manuel; Rossel, Genoveva; Tay, David; McKey, Doyle; Lebot, Vincent

    2013-01-01

    Sweet potato (Ipomoea batatas (L.) Lam., Convolvulaceae) counts among the most widely cultivated staple crops worldwide, yet the origins of its domestication remain unclear. This hexaploid species could have had either an autopolyploid origin, from the diploid I. trifida, or an allopolyploid origin, involving genomes of I. trifida and I. triloba. We generated molecular genetic data for a broad sample of cultivated sweet potatoes and its diploid and polyploid wild relatives, for noncoding chloroplast and nuclear ITS sequences, and nuclear SSRs. Our data did not support an allopolyploid origin for I. batatas, nor any contribution of I. triloba in the genome of domesticated sweet potato. I. trifida and I. batatas are closely related although they do not share haplotypes. Our data support an autopolyploid origin of sweet potato from the ancestor it shares with I. trifida, which might be similar to currently observed tetraploid wild Ipomoea accessions. Two I. batatas chloroplast lineages were identified. They show more divergence with each other than either does with I. trifida. We thus propose that cultivated I. batatas have multiple origins, and evolved from at least two distinct autopolyploidization events in polymorphic wild populations of a single progenitor species. Secondary contact between sweet potatoes domesticated in Central America and in South America, from differentiated wild I. batatas populations, would have led to the introgression of chloroplast haplotypes of each lineage into nuclear backgrounds of the other, and to a reduced divergence between nuclear gene pools as compared with chloroplast haplotypes.

  12. Disentangling the Origins of Cultivated Sweet Potato (Ipomoea batatas (L.) Lam.)

    Science.gov (United States)

    Roullier, Caroline; Duputié, Anne; Wennekes, Paul; Benoit, Laure; Fernández Bringas, Víctor Manuel; Rossel, Genoveva; Tay, David; McKey, Doyle; Lebot, Vincent

    2013-01-01

    Sweet potato (Ipomoea batatas (L.) Lam., Convolvulaceae) counts among the most widely cultivated staple crops worldwide, yet the origins of its domestication remain unclear. This hexaploid species could have had either an autopolyploid origin, from the diploid I. trifida, or an allopolyploid origin, involving genomes of I. trifida and I. triloba. We generated molecular genetic data for a broad sample of cultivated sweet potatoes and its diploid and polyploid wild relatives, for noncoding chloroplast and nuclear ITS sequences, and nuclear SSRs. Our data did not support an allopolyploid origin for I. batatas, nor any contribution of I. triloba in the genome of domesticated sweet potato. I. trifida and I. batatas are closely related although they do not share haplotypes. Our data support an autopolyploid origin of sweet potato from the ancestor it shares with I. trifida, which might be similar to currently observed tetraploid wild Ipomoea accessions. Two I. batatas chloroplast lineages were identified. They show more divergence with each other than either does with I. trifida. We thus propose that cultivated I. batatas have multiple origins, and evolved from at least two distinct autopolyploidization events in polymorphic wild populations of a single progenitor species. Secondary contact between sweet potatoes domesticated in Central America and in South America, from differentiated wild I. batatas populations, would have led to the introgression of chloroplast haplotypes of each lineage into nuclear backgrounds of the other, and to a reduced divergence between nuclear gene pools as compared with chloroplast haplotypes. PMID:23723970

  13. Evaluation of South African bred cultivars of sweetpotato under ...

    African Journals Online (AJOL)

    Sweetpotato (Ipomoea batatas) is widely produced and consumed in Botswana. Identifying cultivars adaptable to the local climatic conditions may stimulate production and give producers and consumers a variety of tuber quality attributes to choose from. Since the country has no capacity to breed its own cultivars, a trial ...

  14. Cassava and sweetpotato yield assessment in Malawi | Moyo ...

    African Journals Online (AJOL)

    Cassava (Manihot esculenta Crantz) and sweetpotato (Ipomoea batatas Lam) are important food and cash crops in Malawi. However, key information regarding varieties grown, sources of planting materials, post-harvest handling, utilisation and tuber yields is lacking. A study was, therefore, conducted to source this ...

  15. Pneumonia intersticial em bovinos associada à ingestão de batata-doce (Ipomoea batatas mofada Interstitial pneumonia in cattle fed moldy sweet potatoes (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Rafael A. Fighera

    2003-12-01

    Full Text Available Uma doença respiratória foi diagnosticada em cinco dentre 23 bovinos (21,7% após terem sido alimentados com batata-doce (Ipomoea batatas mofada em uma pequena propriedade rural em São Vicente do Sul, Rio Grande do Sul, Brasil. Três dos cinco bovinos afetados morreram espontaneamente, e o quarto foi sacrificado para necropsia quando mostrava sinais clínicos respiratórios avançados. A manifestação clínica iniciara cerca de 24 horas após a ingestão das batatas-doces e a evolução clínica foi de 1 a 4 dias. Os sinais clínicos incluíam dispnéia (respiração laboriosa e abdominal, taquipnéia, pescoço estendido com cabeça baixa e dilatação ritmada das narinas. Dois bovinos foram necropsiados. Os achados de necropsia incluíam pulmões distendidos, pálidos e de consistência borrachenta, que não colapsavam quando o tórax era aberto; enfisema e edema acentuados eram evidentes no pulmão. Os linfonodos e o baço apresentavam alterações características de hiperplasia linfóide. Histologica-mente, as lesões eram típicas de pneumonia intersticial. Os septos alveolares estavam espessados por fibroblastos e células inflamatórias, havia hipertrofia e hiperplasia de pneumócitos tipo II; os septos interlobulares estavam distendidos por edema e enfisema. A cultura de amostras das batatas-doces mofadas produziu Fusarium solani e F. oxysporum.Cases of respiratory disease were diagnosed in five out of 23 cattle (21.7% after they were fed moldy damaged sweet potatoes (Ipomoea batatas on a small farm in the county of São Vicente do Sul, state of Rio Grande do Sul, Brazil. Of those five cattle, three died spontaneously and another one was euthanatized for necropsy while showing advanced respiratory clinical signs. The disease manifested itself approximately 24 hours after the ingestion of the sweet potatoes and lasted from 1 to 4 days. Clinical signs included dyspnea (labored breathing and abdominal respiration, tachypnea, extended

  16. Phyotoxicity of diesel soil contamination on the germination of Lactuca sativa and Ipomoea batatas.

    Science.gov (United States)

    Fatokun, Kayode; Lewu, Francis Bayo; Zharare, Godfrey Elijah

    2015-11-01

    Phytotoxic effect of diesel contaminated soil on germination rate of Lactuca sativa and Ipomoea batatas, at two concentrations ranges (0-6ml and 0-30ml), were investigated and compared. Diesel soil contamination was simulated and soil samples were taken from contaminated soil at 1, 5,10, 15, 25, 50, 75 and 100 days should be after planting. The result showed that in both plant species, diesel inhibited germination in a concentration dependent manner, Also, the influence of diesel contamination diminished with increased time duration; suggesting possible reduction in diesel toxicity over time. However, germination of lettuce was significant and negatively correlated (r2 = -0.941) with diesel contamination as compared to sweet potato (r2 = -0.638).Critical concentration of diesel in relation to seed germination of L. sativa was lower than vegetative germination of I. batatas, indicating that germination of I. batatas was less sensitive to diesel contamination as compared to L. sativa.

  17. Seleção de clones de batata-doce resistentes a Meloidogyne incognita raça 1 Selection of sweetpotato clones resistant to Meloidogyne incognita race 1

    Directory of Open Access Journals (Sweden)

    Aline Marchese

    2010-09-01

    Full Text Available O objetivo deste trabalho foi selecionar clones de batata-doce (Ipomoea batatas resistentes à raça 1 de Meloidogyne incognita e avaliar a eficiência do método de seleção empregado, pela estimação dos coeficientes de variação genética e ambiental e das herdabilidades no sentido amplo. Foram utilizados 123 genótipos de batata-doce, entre os quais quatro cultivares comerciais - Brazlândia Rosada, Brazlândia Roxa, Brazlândia Branca e Palmas -, e 119 acessos previamente selecionados no programa de melhoramento vegetal da Universidade Federal de Lavras. O delineamento experimental utilizado foi o de blocos aumentados, com três tratamentos comuns: as cultivares de batata-doce Brazlândia Branca e Palmas, e a cultivar de tomate Santa Clara, suscetível ao nematoide. A classificação dos níveis de resistência foi realizada de acordo com o fator de reprodução do nematoide e o índice de reprodução relativo à cultivar Santa Clara, de tomateiro. A relação entre os coeficientes de variação genética e ambiental e as herdabilidades no sentido amplo foram altas, tanto para o fator de reprodução quanto para o índice de reprodução dos nematoides, o que demonstra a eficiência do método empregado para a seleção de genótipos resistentes. Foram identificados 57 genótipos promissores de batata-doce, resistentes à raça 1 de M. incognita, e selecionados para continuar no programa de melhoramento.The objective of this work was to select sweetpotato (Ipomoea batatas resistant clones to Meloidogyne incognita race 1, and to assess the efficiency of the selection method deployed, through the estimation of genetic and environmental coefficients of variation, and broad-sense heritabilities. Genotypes assessed comprised 123 sweetpotato entries altogether, including four commercial cultivars - Brazlândia Rosada, Brazlândia Roxa, Brazlândia Branca, Palmas - and 119 clones previously selected by the Universidade Federal de Lavras

  18. Recursos genéticos del boniato (Ipomoea batatas (L. Lam. en Cuba. Especies silvestres

    Directory of Open Access Journals (Sweden)

    Oscar Leopoldo Parrado Alvarez

    2013-01-01

    Full Text Available RESUMEN Se presentan los resultados de la valoración de los recursos genéticos del boniato (Ipomoea batatas (L. Lam a partir del estudio de material de herbario y fuentes bibliográficas teniendo en consideración el criterio de grupo taxón de Maxted de acuerdo con el cual en Cuba se reporta la presencia de los grupos siguientes: Grupo Taxón 2 – misma serie o sección que el cultivo (7 especies; Grupo Taxón 3 – mismo subgénero que el cultivo Eriospermum (45 especies dentro de este grupo se encuentran 21 especies endémicas y el Grupo Taxón 4 – mismo género que el cultivo (57 especies lo que potencia a Cuba como un centro de diversidad importante para Ipomoea batatas en Latinoamérica y el Caribe . Se ofrece una descripción sucinta de las especies tratadas que incluye distribución, fenología, datos etnobotánicos y el material de herbario consultado. Genetic resources of sweet potato (Ipomoea batatas (L. lam. in Cuba. Wild species. ABSTRACT The results of the evaluation of the genetic resources of sweet potato (Ipomoea batatas (L. Lam are presented from the study of herbarium material and bibliographic sources taking into account the criterion of Maxted´s taxon group according to which in Cuba is reported the presence of the following groups : Taxon group 2 - same series or section cultivation (7 species Taxon group 3 - same subgenus Eriospermum the crop (45 species within this group are 21 endemic species and Taxon group 4 - same genus as the crop (57 species that fact potenciate to Cuba as an important center of diversity for Ipomoea batatas in Latin America and the Caribbean. A brief description of the treated species including distribution, phenology, ethnobotanical data and herbarium material consulted is provided. A brief description of the treated species including distribution, phenology, ethnobotanical data and herbarium material consulted is provided.

  19. Yield and consumer acceptability of ‘Evangeline’ sweetpotato for production in North Carolina

    Science.gov (United States)

    Studies were conducted in 2012 and 2013 to compare Evangeline to various sweetpotato (Ipomoea batatas) varieties (Bayou Belle, Beauregard, Bonita, Covington, NC05-198, and Orleans) for commercial production in North Carolina. In another study, microwaved and oven-baked ‘Evangeline’ and ‘Covington’ s...

  20. Whitefly transmission of Sweet potato leaf curl virus in sweetpotato germplasm

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lam., is among an extensive number of plant species attacked by Bemisia tabaci (Gennadius). Because this important world food crop is vegetatively propagated, it can conveniently accumulate infections by several viruses. Sweet potato leaf curl virus (SPLCV) (ssDNA...

  1. The performance and profitability of sweet potato ( Ipomoea batatas L.)

    African Journals Online (AJOL)

    This study was conducted in 2013 and 2014 at the Teaching and Research Farm of the Faculty of Agriculture, University of Benin, Benin City, Nigeria. The aim of the study was to evaluate the effect of propagule length and cattle dung application rates on the growth, yield and profitability of sweet potato (Ipomea batatas L.) in ...

  2. growth and yield responses of sweet potato (ipomoea batatas)

    African Journals Online (AJOL)

    DJFLEX

    batatas L. Lam) varieties viz TIS 87/0087 and TIS 8164 to time of potassium (K) fertilizer application in a humid forest zone of .... relative humidity of 75 -85 % . ..... CIP, Lima. Edmond, J.B. 1971. Sweet potato production, processing and marketing. Avi Publishers,. Westport, Connecticut. FAO, 2004. (Food and Agricultural ...

  3. Identificación de virus en el cultivo de camote (Ipomoea batatas L.) en Costa Rica

    OpenAIRE

    Rodrigo Valverde; Marco A. Moreira

    2004-01-01

    Identificación de virus en el cultivo de camote (Ipomoea batatas L.) en Costa Rica. En el período entre febrero y mayo del 2002, se realizó un reconocimiento de los virus del cultivo de camote (Ipomoea batatas L.), en cinco plantaciones comerciales ubicadas en las principales regiones productoras de este cultivo en Costa Rica. Previo al muestreo, en cada plantación se evaluó la incidencia de plantas con síntomas de enfermedades virales. Para la identificación de los virus...

  4. Pemakaian Prebiotik Ubi Jalar Kuning (Ipomoea Batatas L) Dan Probiotik Lactobacillus Casei Pada Pembuatan Susu Fermentasi Sinbiotik

    OpenAIRE

    FAJAR, NAJMIATUL; Indriati, Gustina

    2010-01-01

    This research study about use of prebiotic Ipomea batatas L. and probiotic Lactobacillus casei at milk fermentation. The USAge of simbiotic increase, recentely, especially, for husbandarys products, such as milk fermentation the purpose of this study was to find out the best composition of prebiotic Ipomoea batatas L and probiotic Lactobacillus casei given in the process of simbiotic milk fermentation. The method used for this experiment was random cluster, factorial 4 x 3 with 3 times of r...

  5. IbMADS1 (Ipomoea batatas MADS-box 1 gene) is involved in tuberous root initiation in sweet potato (Ipomoea batatas).

    Science.gov (United States)

    Ku, Amy Tsu; Huang, Yi-Shiuan; Wang, Yu-Shu; Ma, Daifu; Yeh, Kai-Wun

    2008-07-01

    The tuberization mechanism of sweet potato (Ipomoea batatas) has long been studied using various approaches. Morphological data have revealed that the tuberizing events result from the activation of the cambium, followed by cell proliferation. However, uncertainties still remain regarding the regulators participating in this signal-transduction pathway. An attempt was made to characterize the role of one MADS-box transcription factor, which was preferentially expressed in sweet potato roots at the early tuberization stage. A differential expression level of IbMADS1 (Ipomoea batatas MADS-box 1) was detected temporally and spatially in sweet potato tissues. IbMADS1 responses to tuberization-related hormones were assessed. In order to identify the evolutionary significance, the expression pattern of IbMADS1 was surveyed in two tuber-deficient Ipomoea relatives, I. leucantha and I. trifida, and compared with sweet potato. In functional analyses, potato (Solanum tuberosum) was employed as a heterologous model. The resulting tuber morphogenesis was examined anatomically in order to address the physiological function of IbMADS1, which should act similarly in sweet potato. IbMADS1 was preferentially expressed as tuberous root development proceeded. Its expression was inducible by tuberization-related hormones, such as jasmonic acid and cytokinins. In situ hybridization data showed that IbMADS1 transcripts were specifically distributed around immature meristematic cells within the stele and lateral root primordia. Inter-species examination indicated that IbMADS1 expression was relatively active in sweet potato roots, but undetectable in tuber-deficient Ipomoea species. IbMADS1-transformed potatoes exhibited tuber morphogenesis in the fibrous roots. The partial swellings along fibrous roots were mainly due to anomalous proliferation and differentiation in the xylem. Based on this study, it is proposed that IbMADS1 is an important integrator at the initiation of tuberization

  6. Advances in functional use of sweet potato, [Ipomoea batatas (L.) Lam].

    Science.gov (United States)

    Barnes, Sandra L; Sanders, Sheila A

    2012-08-01

    This article reviews the patents that have been presented over the past two decades related to alternative functional use of the Sweet potato, Ipomoea batatas (L.) Lam. The major categories of available patents include alternative food products such as Sweet potato chips and fries, Sweet potato ornamental products, and fuel ethanol production from Sweet potato. The majority of recent patents fall under the category of ornamental products and alternative food products, with only a few fuel ethanol products. Figure 1 shows the major categories of patented alternative products from Sweet potato.

  7. Ether-soluble resin glycosides from the roots of Ipomoea batatas.

    Science.gov (United States)

    Yin, Yong-Qin; Kong, Ling-Yi

    2008-01-01

    Two new resin glycosides, batataosides I (1) and II (2), and five known compounds, friedelin (3), scopoletin (4), octadecyl caffeate (5), beta-sistosterol (6) and daucosterol (7), were isolated from the roots of Ipomoea batatas. Their structures have been determined based on the chemical and spectral data. Batataosides I and II have novel structures because the core simonic acid B was esterised with cinnamic acid for the first time, and three different substituent esterification groups in one resin glycoside is scarce. The absolute configuration of the aglycone was elucidated to be S by Mosher's method.

  8. Somatic embryogenesis in Malaysian cultivars of sweet potato (Ipomoea batatas L.).

    OpenAIRE

    De Silva, Angela Ee

    2017-01-01

    Three Malaysian sweet potato cultivars, namely Ipomoea batatas (L.) cv. Gendut, Jalomas and Telong, were investigated for their abilities to produce somatic embryos. Shoot meristems were used as the starting materials and cultured on Murashige and Skoog basal media (Murashige and Skoog, 1962) in the presence of auxin 2,4-dichlorophenoxyacetic acid (2,4-D) or 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). Auxin 2,4,5-T at 5 µM was most effective for the initiation of primary embryogenic formati...

  9. Challenges to genome sequence dissection in sweetpotato

    Science.gov (United States)

    Isobe, Sachiko; Shirasawa, Kenta; Hirakawa, Hideki

    2017-01-01

    The development of next generation sequencing (NGS) technologies has enabled the determination of whole genome sequences in many non-model plant species. However, genome sequencing in sweetpotato (Ipomoea batatas (L.) Lam) is still difficult because of the hexaploid genome structure. Previous studies suggested that a diploid wild relative, I. trifida (H.B.K.) Don., is the most possible ancestor of sweetpotato. Therefore, the genetic and genomic features of I. trifida have been studied as a potential reference for sweetpotato. Meanwhile, several research groups have begun the challenging task of directly sequencing the sweetpotato genome. In this manuscript, we review the recent results and activities of large-scale genome and transcriptome analysis related to genome sequence dissection in sweetpotato under the sections as follows: I. trifida genome and transcript sequencing, genome sequences of I. nil (Japanese morning glory), transcript sequences in sweetpotato, chloroplast sequences, transposable elements and transfer DNA. The recent international activities of de novo whole genome sequencing in sweetpotato are also described. The large-scale publically available genome and transcript sequence resources and the international genome sequencing streams are expected to promote the genome sequence dissection in sweetpotato. PMID:28465666

  10. Weathering rate of male rubber septa impregnator sex pheromone of Sweetpotato Weevil, Cylas formicarius elegantulus (Coleoptera: Brentidae), in East Hawaii

    Science.gov (United States)

    In recent years, sweet potato, Ipomoea batatas (L.) Lamarck, production in Hawaii has been increasing, reaching 190 harvested ha, with a total production of 3.78 million kg in 2009. Sweet potato production in Hawaii is hindered by three major quarantine pests, for which only one, the sweetpotato we...

  11. Identificación in silico de un grupo de secuencias ortólogas conservadas (COS de Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Christian Solís Calero

    2013-03-01

    Full Text Available En el presente trabajo se describe una serie de procedimientos bioinformáticos para la predicción de un grupo secuencias ortólogas conservadas (COS de Ipomoea batatas, así como la evaluación de su potencial utilidad para la generación de marcadores moleculares y estudios de diversidad en esta especie. Con ese propósito usando los programas BLAST X y TBLASTN se realizó una comparación reciproca por similaridad entre secuencias ESTs procedentes de librerías de cDNAs de Ipomoea batatas, propias o disponibles de modo público en la base de datos GenBank, con secuencias COS de Arabidopsis thaliana. La anotación funcional de las secuencias COS predichas en Ipomoea batatas se realizo usando los programas BLASTX, INTERPROSCAN y PSI-BLAST. Se obtuvieron en total 204 secuencias COS candidatos de Ipomoea batatas, siendo 16 secuencias provenientes de una librería generada a partir de raíces de reserva. Se evaluó de modo computacional el polimorfismo de las secuencias COS de raíces de reserva, obteniéndose SNPs en 8 secuencias, y secuencias repetidas en tandem en una de ellas.

  12. A Functional mathematical index for predicting effects of food processing on eight sweet potato(Ipomoea batatas)cultivars

    Science.gov (United States)

    In this paper we apply an improved functional mathematical index (FMI), modified from those presented in previous publications, to define the influence of different cooking processes of eight sweet potato (Ipomoea batatas) cultivars on composition of six bioactive phenolic compounds (flavonoids). Th...

  13. Characterization of Secondary Metabolites from Purple Ipomoea batatas Leaves and Their Effects on Glucose Uptake.

    Science.gov (United States)

    Lee, Chia-Lin; Lee, Shou-Lun; Chen, Chao-Jung; Chen, Hsin-Chun; Kao, Ming-Ching; Liu, Chuan-Hao; Chen, Jau-Yang; Lai, Yen-Ting; Wu, Yang-Chang

    2016-06-08

    Ipomoea batatas has long been used in folk medicine for the treatment of hyperglycemia or as a food additive for the prevention of type 2 diabetes. However, neither the plant extract nor its active components have been evaluated systematically. In this work four crude extracts, including n-hexane- (IBH), 95% MeOH- (IBM), n-BuOH- (IBB), and H₂O-soluble (IBW) fractions, were prepared by fractionation of a methanolic extract of purple I. batatas leaves. Twenty-four pure compounds 1-24 were then isolated by various chromatographic techniques and their structures identified from NMR and MS data. Glucose uptake assays in differentiated 3T3-L1 adipocytes and rat primary hepatocytes, as well as western blot analysis, were carried out to evaluate the antidiabetic activity of this species. The IBH crude fraction, with methyl decanoate (22) as a major and active compound, showed the greatest effect on glucose uptake, most likely via activation of Glut4 and regulation of the PI3K/AKT pathway. Quercetin 3-O-β-d-sophoroside (1), quercetin (3), benzyl β-d-glucoside (10), 4-hydroxy-3-methoxybenzaldehyde (12), and methyl decanoate (22) could be important components contributing to the antidiabetic effects. We conclude that purple I. batatas leaves have potential as an antidiabetic plant source and the active constituents 1, 3, 10, 12, and 22 are promising lead candidates for future investigation.

  14. Detection and classification of SPLCV isolates in the U.S. sweetpotato germplasm collection via a real-time PCR assay and phylogenetic analysis

    Science.gov (United States)

    The USDA/ARS sweetpotato [Ipomoea batatas (L.) Lam] germplasm collection contains accessions that were initially collected from various countries worldwide. These materials have been maintained and distributed as in vitro plantlets since the 1980s. The status of viral infection by the emerging Swe...

  15. PEMAKAIAN PREBIOTIK UBI JALAR KUNING (Ipomoea batatas L) DAN PROBIOTIK Lactobacillus casei PADA PEMBUATAN SUSU FERMENTASI SINBIOTIK

    OpenAIRE

    NAJMIATUL FAJAR; Gustina Indriati

    2016-01-01

    This research study about use of prebiotic Ipomea batatas L. and probiotic  Lactobacillus casei at milk fermentation. The usage of simbiotic increase, recentely, especially, for husbandarys products, such as milk fermentation the purpose of this study was to find out the best composition of prebiotic  Ipomoea batatas L and probiotic Lactobacillus casei given in the process of simbiotic milk fermentation. The method used for this experiment was random cluster, factorial 4 x 3 with 3 times of r...

  16. The optimal extraction parameters and anti-diabetic activity of flavonoids from Ipomoea batatas leaf.

    Science.gov (United States)

    Li, Fenglin; Li, Qingwang; Gao, Dawei; Peng, Yong

    2009-03-07

    Extraction parameters of flavonoids from Ipomoea batatas leaf (FIBL) and anti-diabetic activity of FIBL on alloxan induced diabetic mice were studied. The optimal extraction parameters of FIBL were obtained by single factor test and orthogonal test, as follows: ethanol concentration 60 %, ratio of solvent to raw material 30, extraction temperature 75 degrees and extraction time 1.5 h, while extraction yield of FIBL was 5.94 %. FIBL treatment (50, 100, and 150 mg/ kg body weight) for 28 days resulted in a significant decrease in the concentration of fasting blood glucose (FBG), total cholesterol (TC) and triglyceride (TG) in diabetes mellitus mice. Furthermore, FIBL significantly increased body weight (bw) and serum high-density lipoprotein cholesterol (HDL-c) level. The data demonstrated FIBL at the dose of 100 mg/kg bw exhibited the optimal effect. The above results suggest that FIBL can control blood glucose and modulate the metabolism of blood lipid in diabetes mellitus mice.

  17. Calf thymus DNA-binding ability study of anthocyanins from purple sweet potatoes ( Ipomoea batatas L.).

    Science.gov (United States)

    Wang, Dan; Wang, Xirui; Zhang, Chao; Ma, Yue; Zhao, Xiaoyan

    2011-07-13

    A total of 10 anthocyanin compounds were identified from five purple sweet potato ( Ipomoea batatas L.) varieties, Qunzi, Zishu038, Ji18, Jingshu6, and Ziluolan, by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to assess their calf thymus DNA-binding ability in vitro. The interaction between anthocyanins and calf thymus DNA in Tris-HCl buffer solution (pH 6.9) was evaluated by fluorescence spectroscopy. Using ethidium bromide (EB) as a fluorescence probe, fluorescence quenching of the emission peak was seen in the DNA-EB system when anthocyanins were added, indicating that the anthocyanins bound with DNA. The acylated groups influenced the ability of the interaction with DNA. Anthocyanins from purple sweet potato with more acylated groups in sorphorose have a stronger binding ability with DNA.

  18. Resin glycosides from the yellow-skinned variety of sweet potato (Ipomoea batatas).

    Science.gov (United States)

    Rosas-Ramírez, Daniel; Pereda-Miranda, Rogelio

    2013-10-02

    Native to tropical America, Ipomoea batatas has been cultivated for over 5000 years in Mexico. The yellow-skinned tuber crop variety, with an orange flesh, has a higher nutritional value than potato. Raw sweet potato can cause a purge due to its resin glycoside content. Purification of the chloroform-soluble resin glycosides from the roots of this variety was accomplished by preparative-scale HPLC, which allowed for the collection of six oligosaccharides, batatin VII (1) and batatinosides VII-IX (2-4), all of novel structure, together with the known resin glycosides pescaprein I and batatinoside IV. High-field NMR spectroscopy and FAB mass spectrometry were used to characterize each structure, identifying operculinic acid A for compounds 2 and 4, and simonic acid B for 3, as their pentasaccharide glycosidic cores. Batatin VII (1) represents a dimer of the know batatinoside IV, consisting of two units of simonic acid B.

  19. Batatins VIII-XI, glycolipid ester-type dimers from Ipomoea batatas.

    Science.gov (United States)

    Rosas-Ramírez, Daniel; Pereda-Miranda, Rogelio

    2015-01-23

    Sweet potato (Ipomoea batatas) is native to the tropics of Central and South America, where many varieties have been consumed for more that 5000 years. In developing countries, this crop is a recognized effective food for fighting malnutrition. Purification of the minor lipophilic glicolipids found in the n-hexane-soluble resin glycosides from the white-skinned variety was performed by preparative-scale recycling HPLC. Application of column overload, peak shaving, heart cutting, and recycling techniques permitted the purification of four new oligosaccharide ester-type dimer derivatives of jalapinolic acid, batatins VIII-XI (1-4). The structural characterization of these complex lipo-oligosaccharides was performed through NMR spectroscopy and MS, indicating that batatins VIII-XI (1-4) possess an oligomeric structure consisting of two pentasaccharide units of the known simonic acid B.

  20. Batatins III-VI, glycolipid ester-type dimers from Ipomoea batatas.

    Science.gov (United States)

    Rosas-Ramírez, Daniel; Escalante-Sánchez, Edgar; Pereda-Miranda, Rogelio

    2011-06-01

    Batatins III-VI (1-4), glycolipid ester-type dimers, were isolated from the tuberous roots of sweet potato (Ipomoea batatas) using recycle high performance liquid chromatography. Their structures were characterized by means of several high-resolution NMR and mass spectrometry techniques. These compounds are the first examples of ester-type dimers which consist of two units of the heterotetrasaccharide operculinic acid C. Each unit was esterified by a different amount and type of acid residues: (2S)-methylbutanoic, cinnamic, decanoic (capric) and dodecanoic (lauric) acids. Batatins III-VI (1-4) are an example of the presence of a large number of resin glycoside congeners in each morning glory species caused by partial acylation of their constitutive saccharide cores. Copyright © 2011 Elsevier Ltd. All rights reserved.

  1. Genetic variability, correlation and path analysis of yield contributing characters in sweet potato (ipomoea batatas lam.)

    International Nuclear Information System (INIS)

    Hossain, M.D.; Rabbani, M.G.; Mollah, M.L.R.

    2000-01-01

    Evaluation of 30 sweet potato (Ipomoea batatas Lam.) genotypes for yield contributing characters and tuber yield per plant revealed high phenotypic and genotypic coefficient of variation (PCV and GCV, respectively) for number of tubers per plant, average tuber weight and tuber yield per plant. The heritability and genetic advance were higher for tuber yield per plant, average tuber weight and number of tubers per plant. These three characters also reflected high heritability as well as high genetic advance. As high positive significant correlation, as well as positive direct effect of average tuber weight and number of tubers per plant on tuber yield per plant were found, these characters should be given prime importance for selecting high yielding sweet potato genotypes. (author)

  2. Sweet potato (Ipomoea batatas [L.] Lam)--a valuable medicinal food: a review.

    Science.gov (United States)

    Mohanraj, Remya; Sivasankar, Subha

    2014-07-01

    Ipomoea batatas (L.) Lam, also known as sweet potato, is an extremely versatile and delicious vegetable that possesses high nutritional value. It is also a valuable medicinal plant having anti-cancer, antidiabetic, and anti-inflammatory activities. Sweet potato is now considered a valuable source of unique natural products, including some that can be used in the development of medicines against various diseases and in making industrial products. The overall objective of this review is to give a bird's-eye view of the nutritional value, health benefits, phytochemical composition, and medicinal properties of sweet potato. Specifically, this review outlines the biological activities of some of the sweet potato compounds that have been isolated, the pharmacological action of the sweet potato extract, clinical studies, and plausible medicinal applications of sweet potato (along with a safety evaluation), and demonstrates the potential of sweet potato as a medicinal food.

  3. Processamento de barras de cereais com adição de farinha de batata-doce (Ipomoea batatas L.) Processing of cereal bars with added sweet potato flour (Ipomea batatas L.)

    OpenAIRE

    Bezerra, José Raniere Mazile Vidal; UNICENTRO; Rigo, Maurício; UNICENTRO; Teixeira, Ângela Moraes; UNICENTRO; Angelo, Mariana Alves; UNICENTRO; Czaikoski, Aline; Graduanda em Engenharia de Alimentos, bolsista Fundação Araucária. BIC/Universidade Estadual do Centro Oeste - UNICENTRO.

    2015-01-01

    Considerando o crescimento do segmento de barras de cereais, a importância da cultura da batata-doce (Ipomoea batatas L.) e seu valor nutricional, este trabalho teve por objetivo a elaboração de barras de cereais com adição de diferentes teores de farinha de batata-doce e sua avaliação de aceitação sensorial, e ainda, a determinação da composição proximal da formulação melhor avaliada sensorialmente. A farinha da batata-doce e as barras de cereais adicionadas a farinha foram submetidas às det...

  4. Sensorial, physical and chemical evaluation of bio-fortified Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Mara N.G. Santos

    2014-08-01

    Full Text Available Context: Bio-fortified sweet potato, Ipomoea batatas (L. Lamarck beta-carotene rich, has been included in the most vulnerable population’s diet with the purpose of reducing health disturbances associated with hipovitaminosis. Aims:To evaluate a physical and chemical analysis of total carotenes and beta-carotene and to determine the antioxidant potential as well as to carry out a sensorial analysis of the bio-fortified Ipomoea batatas (L. Lamarck (BDB in nature and processed. Methods: BDB was processed as sweet (in natura, paste and syrup and physicochemical characteristics were compared, total carotenoids, beta-carotene, antioxidant activity (DPPH and microbiological and sensory analyses were performed. Results:The analysis of total carotenoids, beta-carotene and anti-oxidant potential showed the following results: BDB in nature – 11.81 mg/100 g/440.5 mg/100 g/26.30%; sweet paste – 0.61 mg/100 g/53.5 mg/100 g/53.40% and savored syrup – 0.85 mg/100 g/46.0 mg/100 g/14.30%. The methods of conservation avoided the coliforms growth at 35 and 45°C, Staphylococcus coagulasa positivo, Bacillus cereus and Salmonella in all elaborated candies. The sensorial analysis didn’t detect significant differences among the syrup or paste. Conclusions: The conservation methods have an important function keeping the physical, chemical characteristics and sensorial of BDB, although they can influence in their chemical and nutritional composition, mainly in relation to the quantity of total carotenoids and beta-carotene.

  5. Changes in electrophoretic profiles of Ipomoea batatas (sweet potato induced by gamma radiation

    Directory of Open Access Journals (Sweden)

    Celso Luiz Salgueiro Lage

    2002-06-01

    Full Text Available The ability of nodal segments of Ipomoea batatas to differentiate shoots and roots was evaluated after gamma irradiation. Shoot differentiation was less sensitive to irradiation than roots. However, at 90 Gy, no shoot was able to regenerate a new plant; in contrast 76 % of the roots from irradiated nodal segments continued to grow. The gamma radiation also induced changes in electrophoretic profiles of peroxidases of storage roots. Plants originated from irradiated storage roots presented changes in leaf peroxidase profiles very similar to those produced by leaves directly irradiated. The peroxidase profile of absorbent roots from irradiated storage roots was different from that obtained from directly irradiated absorbent roots.A capacidade de Ipomoea batatas diferenciar parte aérea e raízes foi avaliada após irradiação com raios gama. A diferenciação da parte aérea foi menos sensível que a das raízes. Contudo, na dose de 90 Gy nenhum broto diferenciado regenerou planta, enquanto 76 % das raízes diferenciadas dos segmentos nodais irradiados manteve o crescimento. A radiação também induziu mudanças no perfil elotroforético de isoperoxidases. Plantas originadas de raízes tuberosas irradiadas apresentaram alteração no perfil de isoperoxidases foliares semelhantes ao perfil de folhas diretamente irradiadas. O perfil de isoperoxidases de raízes absorventes irradiadas diretamente não apresentou o mesmo padrão do perfil das raízes absorventes desenvolvidas de raízes tuberosas irradiadas.

  6. Pengaruh Ekstrak Tape Ubi Ungu (Ipomoea Batatas L.) Terhadap Kadar Ast Dan Alt Darah Tikus Setelah Aktifitas Fisik Maksimal

    OpenAIRE

    Wardana, I Made Prasetya; Wijayahadi, Noor

    2015-01-01

    Background : physical activity can cause oxidative stress and tissue damage. Lier is an important organ that has many functions. Physical activity reduces blood perfusion to the liver and can damage liver cells. Consuming exogenous antioxidants is one way to prevent cell damage from oxidative stress. Purple sweet potato (Ipomoea batatas L.) is one source of antioxidants due to the high content of antosianin. Fermentation can increase the stability of antioxidants.Aim : To investigate the effe...

  7. On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity

    OpenAIRE

    Roullier, C; Kambouo, R; Paofa, J; McKey, D; Lebot, V

    2013-01-01

    New Guinea is considered the most important secondary centre of diversity for sweet potato (Ipomoea batatas). We analysed nuclear and chloroplast genetic diversity of 417 New Guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical America. The molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical America. Nuclear data confirm p...

  8. Analyses of the complete genome and gene expression of chloroplast of sweet potato [Ipomoea batata].

    Science.gov (United States)

    Yan, Lang; Lai, Xianjun; Li, Xuedan; Wei, Changhe; Tan, Xuemei; Zhang, Yizheng

    2015-01-01

    Sweet potato [Ipomoea batatas (L.) Lam] ranks among the top seven most important food crops cultivated worldwide and is hexaploid plant (2n=6x=90) in the Convolvulaceae family with a genome size between 2,200 to 3,000 Mb. The genomic resources for this crop are deficient due to its complicated genetic structure. Here, we report the complete nucleotide sequence of the chloroplast (cp) genome of sweet potato, which is a circular molecule of 161,303 bp in the typical quadripartite structure with large (LSC) and small (SSC) single-copy regions separated by a pair of inverted repeats (IRs). The chloroplast DNA contains a total of 145 genes, including 94 protein-encoding genes of which there are 72 single-copy and 11 double-copy genes. The organization and structure of the chloroplast genome (gene content and order, IR expansion/contraction, random repeating sequences, structural rearrangement) of sweet potato were compared with those of Ipomoea (L.) species and some basal important angiosperms, respectively. Some boundary gene-flow and gene gain-and-loss events were identified at intra- and inter-species levels. In addition, by comparing with the transcriptome sequences of sweet potato, the RNA editing events and differential expressions of the chloroplast functional-genes were detected. Moreover, phylogenetic analysis was conducted based on 77 protein-coding genes from 33 taxa and the result may contribute to a better understanding of the evolution progress of the genus Ipomoea (L.), including phylogenetic relationships, intraspecific differentiation and interspecific introgression.

  9. Analyses of the Complete Genome and Gene Expression of Chloroplast of Sweet Potato [Ipomoea batata

    Science.gov (United States)

    Yan, Lang; Lai, Xianjun; Li, Xuedan; Wei, Changhe; Tan, Xuemei; Zhang, Yizheng

    2015-01-01

    Sweet potato [Ipomoea batatas (L.) Lam] ranks among the top seven most important food crops cultivated worldwide and is hexaploid plant (2n=6x=90) in the Convolvulaceae family with a genome size between 2,200 to 3,000 Mb. The genomic resources for this crop are deficient due to its complicated genetic structure. Here, we report the complete nucleotide sequence of the chloroplast (cp) genome of sweet potato, which is a circular molecule of 161,303 bp in the typical quadripartite structure with large (LSC) and small (SSC) single-copy regions separated by a pair of inverted repeats (IRs). The chloroplast DNA contains a total of 145 genes, including 94 protein-encoding genes of which there are 72 single-copy and 11 double-copy genes. The organization and structure of the chloroplast genome (gene content and order, IR expansion/contraction, random repeating sequences, structural rearrangement) of sweet potato were compared with those of Ipomoea (L.) species and some basal important angiosperms, respectively. Some boundary gene-flow and gene gain-and-loss events were identified at intra- and inter-species levels. In addition, by comparing with the transcriptome sequences of sweet potato, the RNA editing events and differential expressions of the chloroplast functional-genes were detected. Moreover, phylogenetic analysis was conducted based on 77 protein-coding genes from 33 taxa and the result may contribute to a better understanding of the evolution progress of the genus Ipomoea (L.), including phylogenetic relationships, intraspecific differentiation and interspecific introgression. PMID:25874767

  10. IPOMOEA BATATAS)

    African Journals Online (AJOL)

    eobe

    c bowl. Six young plants of sweet potato were transplanted to each of the plastic bowl and allowed eight weeks to grow and stabilize. ... grow, sustain growth and survive in the contaminated soil environment; the plant reduced the initial TPH content in the soil from ... Phytoremediation is the use of plants and their associated ...

  11. Immune activity of sweet potato (Ipomoea batatas L.) glycoprotein after enzymatic and chemical modifications.

    Science.gov (United States)

    Xia, Xuejuan; Li, Guannan; Zheng, Jiong; Wu, Jinsong; Kan, Jianquan

    2015-06-01

    This study aimed to investigate the immune activity of sweet potato (Ipomoea batatas L.) glycoprotein (SPG-1) before and after enzymatic and chemical modifications. The protein portion of SPG-1 was modified by pepsin, trypsin, and acetylation treatments. The carbohydrate portion was modified by glucoamylase, NaIO4, and sulfation treatments. The carbohydrate chain of SPG-1 (SPG-1-C) was obtained. Immune activity was analyzed by measuring the serum lysozyme activity and T cell immune response. SPG-1 increased immune activity with a dose-response effect. Immune activity was slightly decreased after pepsin and trypsin hydrolysis, whereas it increased after a moderate degree (DS = 0.68) of acetylation. Immune activity was partly decreased after glucoamylase hydrolysis, remarkably decreased after NaIO4 oxidation, or was lost after a high modification by sulfation. Compared with SPG-1 groups, the SPG-1-C groups increased immune activities had insignificant (P > 0.05) differences. Hence, appropriate modifications of the protein portion could be conducted and it was found that high modifications of the carbohydrate portion should be avoided to improve or maintain the immune function of SPG-1.

  12. Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves’ extract from Ipomoea batatas (L.) Lam

    Science.gov (United States)

    Pochapski, Márcia Thaís; Fosquiera, Eliana Cristina; Esmerino, Luís Antônio; dos Santos, Elizabete Brasil; Farago, Paulo Vitor; Santos, Fábio André; Groppo, Francisco Carlos

    2011-01-01

    Background: Ipomoea batatas (L.) Lam., popularly known as sweet potato (SP), has played an important role as an energy and a phytochemical source in human nutrition and animal feeding. Ethnopharmacological data show that SP leaves have been effectively used in herbal medicine to treat inflammatory and/or infectious oral diseases in Brazil. The aim of this research was to evaluate the phytochemical, antioxidant, and antimicrobial activities of the crude leaves’ extract of SP leaves. Materials and Methods: The screening was performed for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. The color intensity or the precipitate formation was used as analytical responses to these tests. The total antioxidant capacity was evaluated by the phosphomolybdenum complex method. Antimicrobial activity was made by agar disk and agar well diffusion tests. Results: The phytochemical screening showed positive results for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids. Total contents of 345.65, 328.44, and 662.02 mg were respectively obtained for alkaloids, anthraquinones, and phenolic compounds in 100 g of the dry sample. The total antioxidant capacity was 42.94% as compared to ascorbic acid. For antimicrobial studies, no concentration of the SP freeze dried extract was able to inhibit the growth of Streptococcus mutans, S. mitis, Staphylococcus aureus, and Candida albicans in both agar disk and agar well diffusion tests. Conclusions: SP leaves demonstrated the presence of secondary metabolites with potential biological activities. No antimicrobial activity was observed. PMID:21716926

  13. Sweet potato (Ipomoea batatas L.) leaves as nutritional and functional foods.

    Science.gov (United States)

    Sun, Hongnan; Mu, Taihua; Xi, Lisha; Zhang, Miao; Chen, Jingwang

    2014-08-01

    In this study, the nutritional compositions of leaves from 40 sweet potato (Ipomoea batatas L.) cultivars were assessed. The correlations between antioxidant activity and crude protein, crude fat, crude fiber, carbohydrate, and polyphenol contents were determined. The crude protein, crude fiber, crude fat, carbohydrate and ash contents ranged between 16.69-31.08, 9.15-14.26, 2.08-5.28, 42.03-61.36, and 7.39-14.66g/100gdryweight(DW), respectively. According to the index of nutritional quality, sweet potato leaves are good sources of protein, fiber, and minerals, especially K, P, Ca, Mg, Fe, Mn, and Cu. The correlation coefficient between antioxidant activity and total polyphenol content was the highest (0.76032, p<0.0001), indicating that polyphenols are important antioxidants in sweet potato leaves. Sweet potato leaves, which contain several nutrients and bioactive compounds, should be consumed as leafy vegetables in an attempt to reduce malnutrition, especially in developing countries. Copyright © 2014 Elsevier Ltd. All rights reserved.

  14. Aqueous Extract of Ipomoea batatas Reduces Food Intake in Male Wistar Rats: A Pilot Study.

    Science.gov (United States)

    Olubobokun, Th; Aluko, Eo; Iyare, Ee; Anyaehie, Usb; Olatunbosun, Ed; Aizenabor, Gi

    2014-07-01

    Food intake is regulated by the complex interaction of psychological and physiological events associated with ingestion. Food that increases short-term satiety decreases the amount of energy ingested subsequently and thus could potentially help in weight management in the long run. Potato, a common starchy tuber in our environment is believed to contain substances that can help maintain and increases short-term satiety. This study was conducted to determine the effect of the aqueous extract of sweet potato (Ipomoea batatas [IB]) on food intake in male Wistar rats. The sweet potato tubers were chopped into small pieces and homogenized in distilled water for 30 s. Homogenate was filtered through muslin cloth and then centrifuged at 120 rpm for 20 min. For use, the residue was evaporated to dryness. The dried extract was reconstituted in freshly prepared normal saline for administration to test animals. The 20 acclimatized male Wistar rats weighing 170-180 g were used for this study. The animals were randomly assigned into four groups of five rats each. Group 1 served as the control and was fed with 0.3 ml of normal saline while Groups 2-4 were fed with IB extract. The results showed that in the extract-treated groups, the food intake was significantly reduced at P < 0.01 in a dose dependent manner when compared with the control group. Consumption of IB caused a reduction in food intake probably by reducing appetite and increasing satiety.

  15. Chemical optimization of protein extraction from sweet potato (Ipomoea batatas) peel.

    Science.gov (United States)

    Maloney, Katherine P; Truong, Van-Den; Allen, Jonathan C

    2012-11-01

    Proteins isolated from sweet potatoes (Ipomoea batatas) have been shown to possess antidiabetic, antioxidant, and antiproliferative properties. The objective of this study was to chemically optimize a process for extracting proteins from sweet potato peel. The extraction procedure involved mixing peel with saline solvent to dissolve proteins and then precipitating with CaCl(2). Quadratic and segmented models were used to determine the optimum NaCl concentration and peel to solvent ratio to maximize protein solubility while minimizing solvent usage. A segmented model was also used to optimize the concentration of CaCl(2) used for precipitation. The highest yield was obtained by mixing blanched peelings with 59.7 mL of 0.025 mM NaCl per g peel and then precipitating with 6.8 mM CaCl(2). The results of this study show that potentially valuable proteins can be extracted from peel generated during processing of sweet potatoes and industrial costs can be minimized by using these optimum conditions. Potentially valuable proteins can be extracted from sweet potato peel, a waste product of sweet potato processing. © 2012 Institute of Food Technologists®

  16. In vivo wound healing and antiulcer properties of white sweet potato (Ipomoea batatas).

    Science.gov (United States)

    Hermes, Daniele; Dudek, Débora N; Maria, Mariana D; Horta, Lívia P; Lima, Eliete N; de Fátima, Ângelo; Sanches, Andréia C C; Modolo, Luzia V

    2013-07-01

    The potential of tuber flour of Ipomoea batatas (L.) Lam. cv. Brazlândia Branca (white sweet potato) as wound healing and antiulcerogenic agent was investigated in vivo in animal model. Excision on the back of Wistar rats was performed to induce wounds that were topically treated with Beeler's base containing tuber flour of white sweet potato at 2.5%. Number of cells undergoing metaphase and the degree of tissue re-epithelialization were investigated 4, 7 and 10 days post-treatment. The protective effect of aqueous suspension of tuber flour (75 and 100 mg/kg animal weight) on gastric mucosa of Wistar rats was also studied by using the ethanol-induced ulceration model. Ointment based on white sweet potato at 2.5% effectively triggered the healing of cutaneous wound as attested by the increased number of cells undergoing metaphase and tissue re-epithelialization regardless the time of wound treatment. Tuber flour potentially prevented ethanol-induced gastric ulceration by suppressing edema formation and partly protecting gastric mucosa wrinkles. Crude extracts also exhibited potential as free radical scavengers. The results from animal model experiments indicate the potential of tuber flour of white sweet potato to heal wounds.

  17. [Screening of the anti-tumor active fraction from Ipomoea batatas Lam. (cv.simon) leaves].

    Science.gov (United States)

    Lü, Shuhe; Lin, Cong; Xu, Pingsheng

    2015-05-01

    Three fractions (SM, SM-A, SM-B) were prepared from different polarity parts of Ipomoea batatas Lam. (cv.simon) leaves and the anti-tumor potency as well as the dose-response relations were evaluated. The anti-tumor activities of fraction SM, SM-A or SM-B were screened by MTS in human hepatic cancer Hep3B cells, lung cancer A549 cells or gastric carcinoma MGC803 cells, respectively. The three fractions all showed anti-tumor activities in three cancer cells with different sensitivity. Among them, SM-B was the most potent fraction with IC50 values at 15.17 mg/L, 72.64 mg/L or 165.47 mg/L in MGC803 cells, A549 cells or Hep3B cells, respectively (P<0.05). Th e extraction of Brazil sweet potato leaves displayed anti-tumor activity and SM-B was the most potent fraction.

  18. Fractionation, enzyme inhibitory and cellular antioxidant activity of bioactives from purple sweet potato (Ipomoea batatas).

    Science.gov (United States)

    Esatbeyoglu, Tuba; Rodríguez-Werner, Miriam; Schlösser, Anke; Winterhalter, Peter; Rimbach, Gerald

    2017-04-15

    Sweet potato (Ipomoea batatas L.) is mainly cultivated in Asia. The deep purple color of purple sweet potato (PSP) is due to the high content of acylated anthocyanins. In the present study, PSP-derived polyphenols were identified using HPLC-PDA and HPLC-ESI-MS n analyses. After concentration of the polyphenols from PSP, preparative separation into two fractions, designated anthocyanins (AF) and copigments (CF), was carried out using adsorptive membrane chromatography. In enzyme inhibitory assays, all PSP samples inhibited the enzymes α-amylase, α-glucosidase and xanthine oxidase. Additionally, the cell signaling cellular antioxidant properties of the PSP extracts were investigated in cultured cells. PSP induced the transcription factor Nrf2, which regulates the expression of genes encoding heme oxygenase 1 (Hmox1), glutamate-cysteine ligase catalytic subunit (Gclc) and paraoxonase 1 (PON1). Furthermore, PSP enhanced cellular glutathione concentrations and decreased lipid peroxidation in cultured hepatocytes. Overall, these results suggest that PSP extracts exhibit enzyme inhibitory and cellular antioxidant properties, especially PSP CF. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. A proteomic analysis of storage stress responses in Ipomoea batatas (L.) Lam. tuberous root.

    Science.gov (United States)

    Jiang, Yusong; Chen, Cheng; Tao, Xiang; Wang, Jianxi; Zhang, Yizheng

    2012-08-01

    During post-harvest storage, tuberous roots of sweet potato (Ipomoea batatas L. Lam.) usually undergo a biotic and abiotic stress influencing protein expression pattern and substance contents. This research compared the change of total proteins and carbohydrate content in tuberous roots of sweet potato during the storage period. The result of the two-dimensional electrophoresis analysis demonstrated that there were 25 differentially expressed proteins between day 0 and day 75 during the storage. Among these proteins, 11 proteins were down-regulated and the other 14 were up-regulated. The results from MALDI-TOF-TOF/MS analyses and mascot database searching showed that 11 of the 25 differentially expressed proteins were identified as store-stress regulated proteins. It was also found that the proteins involved in the energy metabolism and the stress-response were drastically up-regulated, whereas those in biomacromolecule synthesis were markedly down-regulated. Meanwhile, under the experimental conditions, the content of the starch and the cellulose was decreased by more than a quarter and the amylase activity was increased moderately.

  20. In vivo wound healing and antiulcer properties of white sweet potato (Ipomoea batatas)

    Science.gov (United States)

    Hermes, Daniele; Dudek, Débora N.; Maria, Mariana D.; Horta, Lívia P.; Lima, Eliete N.; de Fátima, Ângelo; Sanches, Andréia C.C.; Modolo, Luzia V.

    2012-01-01

    The potential of tuber flour of Ipomoea batatas (L.) Lam. cv. Brazlândia Branca (white sweet potato) as wound healing and antiulcerogenic agent was investigated in vivo in animal model. Excision on the back of Wistar rats was performed to induce wounds that were topically treated with Beeler’s base containing tuber flour of white sweet potato at 2.5%. Number of cells undergoing metaphase and the degree of tissue re-epithelialization were investigated 4, 7 and 10 days post-treatment. The protective effect of aqueous suspension of tuber flour (75 and 100 mg/kg animal weight) on gastric mucosa of Wistar rats was also studied by using the ethanol-induced ulceration model. Ointment based on white sweet potato at 2.5% effectively triggered the healing of cutaneous wound as attested by the increased number of cells undergoing metaphase and tissue re-epithelialization regardless the time of wound treatment. Tuber flour potentially prevented ethanol-induced gastric ulceration by suppressing edema formation and partly protecting gastric mucosa wrinkles. Crude extracts also exhibited potential as free radical scavengers. The results from animal model experiments indicate the potential of tuber flour of white sweet potato to heal wounds. PMID:25685447

  1. Polyphenols and phenolic acids in sweet potato (Ipomoea batatas L. roots

    Directory of Open Access Journals (Sweden)

    Janette Musilová

    2017-01-01

    Full Text Available Sweet potato (Ipomoea batatas L. is one of the most important food crops in the world. They are rich in polyphenols, proteins, vitamins, minerals and some functional microcomponents. Polyphenols are bioactive compounds, which can protect the human body from the oxidative stress which may cause many diseases including cancer, aging and cardiovascular problems.The polyphenol content is two to three times higher than in some common vegetables. Total polyphenols (determined spectrophotometrically and phenolic acids (i.e. caffeic acid, chlorogenic acid and isomers - using high performance liquid chromatography contents were determined in three varieties of sweet potatoes (O´Henry - white, Beauregard-orange and 414-purple. Phenolic compounds contents were determined in raw peeled roots, jackets of raw roots and water steamed sweet potato roots. For all analysis lyophilised samples were used. Total polyphenol content ranged from 1161 (O´Henry, flesh-raw to 13998 (414, peel-raw mg.kg-1 dry matter, caffeic acid content from the non-detected values (414, flesh-raw to 320.7 (Beauregard, peel-raw mg.kg-1 dry matter and 3-caffeoylquinic acid content from 57.57 (O´Henry, flesh-raw to 2392 (414, peel-raw mg.kg-1 dry matter. Statistically significant differences (p ≤0.05 existed between varieties, morphological parts of the root, or raw and heat-treated sweet potato in phenolic compounds contents.

  2. Functional components in sweetpotato and their genetic improvement

    Science.gov (United States)

    Tanaka, Masaru; Ishiguro, Koji; Oki, Tomoyuki; Okuno, Shigenori

    2017-01-01

    In addition to the nutritionally important components such as starches, vitamins and minerals, storage roots and leaves of sweetpotato (Ipomoea batatas) contains several components with health-promoting functions. Of these, the functionalities of carotenoids, anthocyanins and caffeoylquinic acids have been well established by in vitro and in vivo experiments. Several sweetpotato cultivars containing high levels of these components have been developed in Japan; e.g., ‘Ayamurasaki’, which has high amounts of anthocyanin in its storage roots. To further improve the content and also to change the composition of these functional components, the identification of the genes involved in their biosynthesis and genetic modification of the biosynthetic pathway has been attempted. In this review, we summarize the present status of the research and breeding for these functional components, and we discuss the future prospects for improving sweetpotato functionality. PMID:28465668

  3. AMIDO DE BATATA DOCE (Ipomoea batatas L, Lam: II – CARACTERIZAÇÃO MORFOLÓGICA E ESTUDO DE ALGUMAS PROPRIEDADES FUNCIONAIS

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    José Paschoal BATISTUTI

    2009-07-01

    Full Text Available

    RESUMO: O objetivo deste trabalho foi caracterizar morfologicamente e estudar a relação estrutura: função do grânulo de amido extraído de tubérculos, de diferentes cultivares de batata-doce (Ipomoea batatas L, Lam. Nas condições do experimento e mediante os resultados obtidos, observou-se que o amido dos diferentes cultivares estudados mostrou diferenças acentuadas na sua morfologia. Foram observadas, também, diferenças nas propriedades viscoamilográficas, devidas particularmente à heterogeneidade de forças envolvidas na manutenção da estrutura do grânulo. O amido obtido, dos cultivares estudados, mostrou maior resistência à desintegração mecânica, durante a gelatinização, quando comparado aos padrões comerciais testados. Apenas um cultivar mostrou pico de viscosidade máxima. PALAVRAS-CHAVE: Batata-doce; amido; funcionalidade; morfologia.

  4. Malt hydrolysis of sweet-potatoes and eddoes for ethanol production

    Energy Technology Data Exchange (ETDEWEB)

    Hosein, Rhonda; Mellowes, W.A. (University of the West Indies, St. Augustine, Trinidad. Dept. of Chemical Engineering)

    1989-01-01

    In the Caribbean the main root crops produced are cassava, sweet-potatoes, eddoes, dasheen and yam. The production of ethanol from these starchy substrates first requires the hydrolysis of the starch into simpler sugars. Hydrolysis can be performed enzymatically or by means of acids. The root crops selected for study were sweet-potatoes (Ipomoea batatas) and eddoes (Colocasia antiquorum esculenta var. globulifera). They were hydrolysed using the enzymes contained in malt. The sugars obtained under the above conditions were 5.6 and 5.4% (w/v) for sweet-potatoes and eddoes, respectively. The corresponding starch conversions were 88 and 92%. Fermentation of the above hydrolysates gave alcohol in the region of 2.3 and 2.2% (v/v) for sweet-potatoes and eddoes, respectively. The conversion of sugar to alcohol was 91 and 89%. (author).

  5. Plant-growth regulators alter phytochemical constituents and pharmaceutical quality in Sweet potato (Ipomoea batatas L.).

    Science.gov (United States)

    Ghasemzadeh, Ali; Talei, Daryush; Jaafar, Hawa Z E; Juraimi, Abdul Shukor; Mohamed, Mahmud Tengku Muda; Puteh, Adam; Halim, Mohd Ridzwan A

    2016-05-28

    Sweet potato (Ipomoea batatas L.) is one of the most important consumed crops in many parts of the world because of its economic importance and content of health-promoting phytochemicals. With the sweet potato (Ipomoea batatas L.) as our model, we investigated the exogenous effects of three plant-growth regulators methyl jasmonate (MeJA), salicylic acid (SA), and abscisic acid (ABA) on major phytochemicals in relation to phenylalanine ammonia lyase (PAL) activity. Specifically, we investigated the total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and total β-carotene content (TCC). Individual phenolic and flavonoid compounds were identified using ultra-high performance liquid chromatography (UHPLC). Antioxidant activities of treated plants were evaluated using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and a β-carotene bleaching assay. Anticancer activity of extracts was evaluated against breast cancer cell lines (MCF-7 and MDA-MB-231) using MTT assay. TPC, TFC, TAC, and TCC and antioxidant activities were substantially increased in MeJA-, SA-, and ABA-treated plants. Among the secondary metabolites identified in this study, MeJA application significantly induced production of quercetin, kaempferol, myricetin, gallic acid, chlorogenic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid. Luteolin synthesis was significantly induced by SA application. Compared with control plants, MeJA-treated sweet potato exhibited the highest PAL activity, followed by SA and ABA treatment. The high DPPH activity was observed in MeJA followed by SA and ABA, with half-maximal inhibitory concentration (IC50) values of 2.40, 3.0, and 3.40 mg/mL compared with α-tocopherol (1.1 mg/mL). Additionally, MeJA-treated sweet potato showed the highest β-carotene bleaching activity, with an IC50 value of 2.90 mg/mL, followed by SA (3.30 mg/mL), ABA (3.70 mg/mL), and control plants (4.5 mg/mL). Extracts of sweet potato root treated

  6. Structure of sweet potato (Ipomoea batatas diversity in West Africa covaries with a climatic gradient.

    Directory of Open Access Journals (Sweden)

    Kodjo Glato

    Full Text Available Sub-Saharan agriculture has been identified as vulnerable to ongoing climate change. Adaptation of agriculture has been suggested as a way to maintain productivity. Better knowledge of intra-specific diversity of varieties is prerequisites for the successful management of such adaptation. Among crops, root and tubers play important roles in food security and economic growth for the most vulnerable populations in Africa. Here, we focus on the sweet potato. The Sweet potato (Ipomoea batatas was domesticated in Central and South America and was later introduced into Africa and is now cultivated throughout tropical Africa. We evaluated its diversity in West Africa by sampling a region extending from the coastal area of Togo to the northern Sahelian region of Senegal that represents a range of climatic conditions. Using 12 microsatellite markers, we evaluated 132 varieties along this gradient. Phenotypic data from field trials conducted in three seasons was also obtained. Genetic diversity in West Africa was found to be 18% lower than in America. Genetic diversity in West Africa is structured into five groups, with some groups found in very specific climatic areas, e.g. under a tropical humid climate, or under a Sahelian climate. We also observed genetic groups that occur in a wider range of climates. The genetic groups were also associated with morphological differentiation, mainly the shape of the leaves and the color of the stem or root. This particular structure of diversity along a climatic gradient with association to phenotypic variability can be used for conservation strategies. If such structure is proved to be associated with specific climatic adaptation, it will also allow developing strategies to adapt agriculture to ongoing climate variation in West Africa.

  7. Structure of sweet potato (Ipomoea batatas) diversity in West Africa covaries with a climatic gradient.

    Science.gov (United States)

    Glato, Kodjo; Aidam, Atsou; Kane, Ndjido Ardo; Bassirou, Diallo; Couderc, Marie; Zekraoui, Leila; Scarcelli, Nora; Barnaud, Adeline; Vigouroux, Yves

    2017-01-01

    Sub-Saharan agriculture has been identified as vulnerable to ongoing climate change. Adaptation of agriculture has been suggested as a way to maintain productivity. Better knowledge of intra-specific diversity of varieties is prerequisites for the successful management of such adaptation. Among crops, root and tubers play important roles in food security and economic growth for the most vulnerable populations in Africa. Here, we focus on the sweet potato. The Sweet potato (Ipomoea batatas) was domesticated in Central and South America and was later introduced into Africa and is now cultivated throughout tropical Africa. We evaluated its diversity in West Africa by sampling a region extending from the coastal area of Togo to the northern Sahelian region of Senegal that represents a range of climatic conditions. Using 12 microsatellite markers, we evaluated 132 varieties along this gradient. Phenotypic data from field trials conducted in three seasons was also obtained. Genetic diversity in West Africa was found to be 18% lower than in America. Genetic diversity in West Africa is structured into five groups, with some groups found in very specific climatic areas, e.g. under a tropical humid climate, or under a Sahelian climate. We also observed genetic groups that occur in a wider range of climates. The genetic groups were also associated with morphological differentiation, mainly the shape of the leaves and the color of the stem or root. This particular structure of diversity along a climatic gradient with association to phenotypic variability can be used for conservation strategies. If such structure is proved to be associated with specific climatic adaptation, it will also allow developing strategies to adapt agriculture to ongoing climate variation in West Africa.

  8. Growth and yield response of sweet potato to atmospheric CO/sub 2/ enrichment. [Ipomoea batatas

    Energy Technology Data Exchange (ETDEWEB)

    Bhattacharya, N.C.; Biswas, P.K.; Bhattacharya, S.; Sionit, N.; Strain, B.R.

    Tuber growth of sweet potato (Ipomoea batatas) is a sink that may be limited by source capacity under present ambient CO/sub 2/ levels. Hence, sweet potato may demonstrate more response to predicted increases in atmospheric CO/sub 2/ than many other annual plants. The present investigation was undertaken to determine the long-term effects of CO/sub 2/ enrichment on some physiological parameters, growth, and yield, as well as on the source-sink relationship in sweet potato at different stages of growth. Plants of the cultivar Georgia Jet were grown in controlled environment chambers at 350, 675, and 1000 ..mu..L L/sup -1/ CO/sub 2/. The temperature was 28/sup 0/C during 14-h days and 20/sup 0/C during 10-h nights. The photosynthetic photon flux density was 550 ..mu..mol m/sup -2/ s/sup -1/. The length of main stem, total branch length, number of branches, and leaf area were increased for plants grown at 675 or 1000 ..mu..L L/sup -1/ CO/sub 2/. The production of total dry matter of plants increased at each harvest interval in response to CO/sub 2/ enrichment but it was greatest in 1000 ..mu..L L/sup -1/ CO/sub 2/. Specific leaf weight also increased with increased CO/sub 2/ concentration. The number and diameter of tubers increased at high CO/sub 2/ concentration. At the final harvest, the dry weight of roots and tubers increased 1.8 and 2.6 times in plants grown at 675 and 1000 ..mu..L L/sup -1/ CO/sub 2/, respectively, compared to those grown at 350 ..mu..L L/sup -1/ CO/sub 2/. Carbon dioxide enrichment resulted in the modulation of sink capacity to enhance the production of tubers in sweet potato.

  9. Multiple biological functions of sporamin related to stress tolerance in sweet potato (Ipomoea batatas Lam).

    Science.gov (United States)

    Senthilkumar, Rajendran; Yeh, Kai-Wun

    2012-01-01

    The initial investigation of the nature of the proteins in the tuber of sweet potato (Ipomoea batatas Lam.) revealed a globulin-designated "ipomoein," which was reported by Jones and Gersdorff, (1931). Later, "ipomoein" was renamed "sporamin" and was found to be a major storage protein that accounted for over 80% of the total protein in the tuberous root. To date, sporamin has been studied by a series of biochemical and molecular approaches. The first purification of sporamin into two major fractions, A and B, was successfully completed in 1985. Several characteristics of the protein, such as the diversification of the nucleotide sequences in the gene family, the protein structure, the biological functions of storage, defense, inhibitory activity and ROS scavenging, were identified. In the past decade, sporamin was classified as a Kunitz-type trypsin inhibitor, and its insect-resistance capability has been examined in transgenic tobacco and cauliflower plants, indicating the multiple functions of this protein has evolved to facilitate the growth and development of sweet potato. Sporamin is constitutively expressed in the tuberous root and is not normally expressed in the stem or leaves. However, this protein is expressed systemically in response to wounding and other abiotic stresses. These dual expression patterns at the transcriptional level revealed that the complex regulatory mechanism of sporamin was modulated by environmental stresses. The versatile functions of sporamin make this storage protein a good research model to study molecular evolution, regulatory mechanisms and physiological functions in plants. This review summarizes and discusses recent approaches and future perspectives in agricultural biotechnology. Copyright © 2012 Elsevier Inc. All rights reserved.

  10. Two rubisco activase genes from ipomoea batatas have different roles in photosynthesis of arabidopsis

    International Nuclear Information System (INIS)

    Jiang, Y.

    2014-01-01

    Rubisco activase (RCA) that functions as a molecular chaperone regulates the activity of the Calvin-Benson cycle via regulation of the Rubisco activity. In plants such as Arabidopsis thaliana, Spinacia oleracea, and Oryza sativa, there are two RCA isoforms from two mRNAs that are produced from alternative splicing of the transcribed pre-mRNA of a single RCA gene. However, this research reported that the transcripts of the two IbRCA isoforms in sweet potato (Ipomoea batatas) were transcribed from two different genes. To study the roles of these two IbRCA isoforms in photosynthesis, we inserted these two IbRCA genes into the genome of Arabidopsis with deletion of RCA gene (RCA), resulting in IbRCAs- and IbRCAl-expressing plants, respectively. Analysis of these transgenic Arabidopsis indicated that the IbRCAs-expressing plants were similar to wild-type plants under ambient CO/sub 2/ concentration and 22 degree C conditions, suggesting that expression of IbRCAs gene was sufficient for functional complementation of RCA plants under normal conditions. However, IbRCAs-expressing plants were more susceptible to moderate heat stress (26 degree C) compared to wild-type plants. In contrast, although the IbRCAl-expressing plants had to grow normally in high CO/sub 2/ concentration conditions, there were almost no differences in growth and photosynthesis between normally grown and heat-treated plants, implying that IbRCAl-expressing plants had a better heat-resistance than IbRCAs-expressing plants. (author)

  11. Structure of sweet potato (Ipomoea batatas) diversity in West Africa covaries with a climatic gradient

    Science.gov (United States)

    Aidam, Atsou; Kane, Ndjido Ardo; Bassirou, Diallo; Couderc, Marie; Zekraoui, Leila; Scarcelli, Nora; Barnaud, Adeline; Vigouroux, Yves

    2017-01-01

    Sub-Saharan agriculture has been identified as vulnerable to ongoing climate change. Adaptation of agriculture has been suggested as a way to maintain productivity. Better knowledge of intra-specific diversity of varieties is prerequisites for the successful management of such adaptation. Among crops, root and tubers play important roles in food security and economic growth for the most vulnerable populations in Africa. Here, we focus on the sweet potato. The Sweet potato (Ipomoea batatas) was domesticated in Central and South America and was later introduced into Africa and is now cultivated throughout tropical Africa. We evaluated its diversity in West Africa by sampling a region extending from the coastal area of Togo to the northern Sahelian region of Senegal that represents a range of climatic conditions. Using 12 microsatellite markers, we evaluated 132 varieties along this gradient. Phenotypic data from field trials conducted in three seasons was also obtained. Genetic diversity in West Africa was found to be 18% lower than in America. Genetic diversity in West Africa is structured into five groups, with some groups found in very specific climatic areas, e.g. under a tropical humid climate, or under a Sahelian climate. We also observed genetic groups that occur in a wider range of climates. The genetic groups were also associated with morphological differentiation, mainly the shape of the leaves and the color of the stem or root. This particular structure of diversity along a climatic gradient with association to phenotypic variability can be used for conservation strategies. If such structure is proved to be associated with specific climatic adaptation, it will also allow developing strategies to adapt agriculture to ongoing climate variation in West Africa. PMID:28552989

  12. Identificación de virus en el cultivo de camote (Ipomoea batatas L. en Costa Rica

    Directory of Open Access Journals (Sweden)

    Rodrigo Valverde

    2004-01-01

    Full Text Available Identificación de virus en el cultivo de camote (Ipomoea batatas L. en Costa Rica. En el período entre febrero y mayo del 2002, se realizó un reconocimiento de los virus del cultivo de camote (Ipomoea batatas L., en cinco plantaciones comerciales ubicadas en las principales regiones productoras de este cultivo en Costa Rica. Previo al muestreo, en cada plantación se evaluó la incidencia de plantas con síntomas de enfermedades virales. Para la identificación de los virus, se recolectaron secciones apicales de tallo de plantas de camote con síntomas las cuales se injertaron sobre las plantas indicadoras de virus, de la especia Ipomoea setosa para inocularlas. Las muestras foliares de plantas inoculadas fueron analizadas serológicamente para el virus del moteado plumoso del camote (SPFMV y el virus del mosaico del pepino (CMV; con la reacción en cadena de polimerasa de transcripción reversa (RT-PCR para el virus del enanismo clorótico del camote (SPCSV y por hibridación molecular para el virus del enrollamiento de la hoja del camote (SPLCV. Las plantaciones ubicadas en el Cacao y el Coyol de Alajuela, y San Pedro de Santa Bárbara, presentaron las mayores incidencias de plantas con síntomas virales; a saber: 30, 90 y 90% respectivamente. El SPFMV, transmitido por los áfidos fue el virus más común. Otros virus detectados fueron el SPCSV y el SPLCV. El CMV, no se encontró en ninguna de las muestras analizadas.

  13. Segregation of Hydroxycinnamic Acid Esters Mediating Sweetpotato Weevil Resistance in Storage Roots of Sweetpotato

    Science.gov (United States)

    Anyanga, Milton O.; Yada, Benard; Yencho, G. C.; Ssemakula, Gorrettie N.; Alajo, Agnes; Farman, Dudley I.; Mwanga, Robert O. M.; Stevenson, Philip C.

    2017-01-01

    Resistance to sweetpotato weevils (Cylas spp.) has been identified in several sweetpotato (Ipomoea batatas) landraces from East Africa and shown to be conferred by hydroxycinnamic acids that occur on the surface of storage roots. The segregation of resistance in this crop is unknown and could be monitored using these chemical traits as markers for resistance in F1 offspring from breeding programs. For the first time in a segregating population, we quantified the plant chemicals that confer resistance and evaluated levels of insect colonization of the same progeny in field and laboratory studies. We used a bi-parental mapping population of 287 progenies from a cross between I. batatas ‘New Kawogo,’ a weevil resistant Ugandan landrace and I. batatas ‘Beauregard’ a North American orange-fleshed and weevil susceptible cultivar. The progenies were evaluated for resistance to sweetpotato weevil, Cylas puncticollis at three field locations that varied climatically and across two seasons to determine how environment and location influenced resistance. To augment our field open-choice resistance screening, each clone was also evaluated in a no choice experiment with weevils reared in the laboratory. Chemical analysis was used to determine whether differences in resistance to weevils were associated with plant compounds previously identified as conferring resistance. We established linkage between field and laboratory resistance to Cylas spp. and sweetpotato root chemistry. The data also showed that resistance in sweetpotato was mediated by root chemicals in most but not all cases. Multi-location trials especially from Serere data provided evidence that the hydroxycinnamic acid esters are produced constitutively within the plants in different clonal genotypes and that the ecological interaction of these chemicals in sweetpotato with weevils confers resistance. Our data suggest that these chemical traits are controlled quantitatively and that ultimately a knowledge of

  14. PEMANFAATAN UBI JALAR UNGU (Ipomoea batatas L. Poir SEBAGAI PENGGANTI SEBAGIAN TEPUNG TERIGU DAN SUMBER ANTIOKSIDAN PADA ROTI TAWAR [Purple Sweet Potato (Ipomoea batatas L. Poir as a Partial Subtitute of Wheat flour and Source of Antioxidant on Plain Bread

    Directory of Open Access Journals (Sweden)

    Hardoko*

    2010-06-01

    Full Text Available Purple sweet potato (Ipomoea batatas L. Poir is potential for functional food, especially as antioxidant source due to its purple color and other nutrient content. The objective of this research was to produce plain bread containing antioxidant by partial substitution of wheat flour with purple sweet potato flour. The result showed that the best plain bread was produced by a maximum substitution of 20% purple sweet potato flour to wheat flour. The hedonic characteristics of the resulting bread including aroma, taste, and texture were not significantly different from bread without substitution. Nevertheless, the crust was harder and darker. Addition of 1.0% GMS emulsifier and reduction of purple potato flour to 15% increased the score of softness, hedonic texture, acceptance level, as well as the volume of the bread. The substituted bread had antioxidant activity of 55833.78 ppm DPPH as shown by the IC50 value and contained 4.30% of dietary fiber.

  15. Obtención de Maltodextrinas por vía enzimática a partir del almidón de camote (Ipomoea batatas).

    OpenAIRE

    Medina García, Luis

    2013-01-01

    El objetivo del presente trabajo de investigación consistió en producir maltodextrinas por vía enzimática a partir del almidón del camote (Ipomoea batatas); materia prima que es ampliamente cultivada en varios estados de la República Mexicana. 5 Inicialmente se realizó el análisis químico proximal de los tubérculos del camote (Ipomoea batatas variedad nylon), después se llevó a cabo la extracción y caracterización del almidón y posteriormente se determinaron las condiciones de hidrólisi...

  16. The effects of an arabinogalactan-protein from the white-skinned sweet potato (Ipomoea batatas L.) on blood glucose in spontaneous diabetic mice.

    Science.gov (United States)

    Oki, Naoko; Nonaka, Seiko; Ozaki, Sei

    2011-01-01

    We examined the effects of an arabinogalactanprotein (WSSP-AGP) from Ipomoea batatas L. on hyperglycemia in db/db mice. An oral glucose tolerance test indicated significantly decreased plasma glucose levels by WSSP-AGP. Additionally, an insulin tolerance test found improvement in insulin sensitivity due to treatment with WSSP-AGP. This suggests that amelioration of insulin resistance by WSSP-AGP causes to lead its hypoglycemic effects.

  17. Sweet potato (Ipomoea batatas L. growing in conditions of southern Slovak republic

    Directory of Open Access Journals (Sweden)

    Miroslav Šlosár

    2016-07-01

    Full Text Available The sweet potato (Ipomoea batatas L. belongs to very important crops from aspect of its world production. It is grown in large areas in Asia, on the contrary, sweet potato production in Europe presents minimal part of its total world rate. The sweet potato is less-known crop, grown only on small area in home gardens in Slovak Republic. Tubers of sweet potato are characterized by anti-diabetic, anti-oxidant and anti-proliferative properties due to the presence of valuable health-promoting components, such as carotenoids or vitamin C. The main objective of study was testing of sweet potato growing in conditions of southern Slovak Republic with focus on quantity and quality of its yield. The field trial was realised on land of the Slovak University of Agriculture in Nitra in 2015. Within trial, effect of cultivar and mulching on the selected quantitative (average tuber weight; yield per plant; yield in t.ha-1 and qualitative (total carotenoids; vitamin C parameters were tested. One certified cultivar of sweet potato 'Beauregard' was used as a comparative cultivar. Other two cultivars were marked according to the market place at which were purchased and sequentially used for seedling preparation. Tubers of first un-known cultivar were purchased in the Serbian market (marked as 'Serbian'. Tubers of next sweet potato cultivar were purchased on the market in Zagreb (marked as 'Zagrebian'. Outplating of sweet potato seedlings were realised on the 19th May 2015. The sweet potato was grown by hillock system. Each cultivar was planted in two variants (rows: non-mulching (bare soil and mulching by black non-woven textile. All variants were divided to three replications with 6 plants. Difference between rows was 1.20 m and seedlings were planted in distance of 0.30 m in row. The harvested tubers were classified in two size classes: >150 g (marketable yield and <150 g (non-marketable yield. Total carotenoid content was determined spectrophotometrically. The

  18. Production of 4-ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of Ipomoea batatas Lam.: a comparative study.

    Science.gov (United States)

    Mohanraj, Remya; Subha, S

    2015-05-01

    A comparative study was done on the production of 4-ipomeanol from root tubers of Ipomoea batatas and its rhizogenic callus. Best callusing response was obtained on MS medium supplemented with 11 μM NAA (α-Naphthaleneacetic acid) and 1 μM KIN (Kinetin). Effect of various elicitors (Fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. Methanol extract of the samples were purified by column chromatography and detected using TLC. Identification of 4-ipomeanol was confirmed using HPLC and quantified spectrophotometrically. A mass spectrum was recorded to confirm the presence of 4-ipomeanol. The calli grown under chitin produced highest (6.61mg g(-1)) amount of 4-ipomeanol. This is the first report on in vitro production of 4-ipomeanol from I. batatas. Since 4-ipomeanol is reported to be present only in I. batatas, this study would help in standardizing protocols for large scale production without affecting its natural flora.

  19. Recent progress in sweetpotato breeding and cultivars for diverse applications in Japan

    Science.gov (United States)

    Katayama, Kenji; Kobayashi, Akira; Sakai, Tetsufumi; Kuranouchi, Toshikazu; Kai, Yumi

    2017-01-01

    Sweetpotato (Ipomoea batatas (L.) Lam.) is an outcrossing hexaploid that is cultivated in the tropics and warm-temperate regions of the world. Sweetpotato has played an important role as a famine-relief crop during its long history and has recently been reevaluated as a health-promoting food. In Japan, sweetpotato is used for a wide range of applications, such as table use, processed foods, and alcohol and starch production, and two groups at National Agriculture Research Organization (NARO) undertake the breeding of cultivars for these applications. Sweetpotato breeders utilize breeding processes such as grafting for flower induction and the identification of incompatibility groups before crossing to conquer problems peculiar to sweetpotato. For table use, new cultivars with high sugar content were released recently and have become popular among Japanese consumers. New cultivars with high anthocyanin or β-carotene content were released for processed foods and use as colorants. As raw materials, new cultivars with high alcohol yield were released for the production of shochu spirits. In addition, new cultivars with high starch yield and a cultivar containing starch with excellent cold-storage ability were released for starch production. This review deals with recent progress in sweetpotato breeding and cultivars for diverse applications in Japan. PMID:28465663

  20. Integrating biological treatment of crop residue into a hydroponic sweetpotato culture

    Science.gov (United States)

    Trotman, A. A.; David, P. P.; Bonsi, C. K.; Hill, W. A.; Mortley, D. G.; Loretan, P. A.

    1997-01-01

    Residual biomass from hydroponic culture of sweetpotato [Ipomoea batatas (L.) Lam.] was degraded using natural bacterial soil isolates. Sweetpotato was grown for 120 days in hydroponic culture with a nutrient solution comprised of a ratio of 80% modified half Hoagland solution to 20% filtered effluent from an aerobic starch hydrolysis bioreactor. The phytotoxicity of the effluent was assayed with `Waldmann's Green' lettuce (Lactuca sativa L.) and the ratio selected after a 60-day bioassay using sweetpotato plants propagated vegetatively from cuttings. Controlled environment chamber experiments were conducted to investigate the impact of filtrate from biological treatment of crop residue on growth and storage root production with plants grown in a modified half Hoagland solution. Incorporation of bioreactor effluent, reduced storage root yield of `Georgia Jet' sweetpotato but the decrease was not statistically significant when compared with yield for plants cultured in a modified half Hoagland solution without filtrate. However, yield of `TU-82-155' sweetpotato was significantly reduced when grown in a modified half Hoagland solution into which filtered effluent had been incorporated. Total biomass was significantly reduced for both sweetpotato cultivars when grown in bioreactor effluent. The leaf area and dry matter accumulation were significantly (P < 0.05) reduced for both cultivars when grown in solution culture containing 20% filtered effluent.

  1. On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity.

    Science.gov (United States)

    Roullier, C; Kambouo, R; Paofa, J; McKey, D; Lebot, V

    2013-06-01

    New Guinea is considered the most important secondary centre of diversity for sweet potato (Ipomoea batatas). We analysed nuclear and chloroplast genetic diversity of 417 New Guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical America. The molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical America. Nuclear data confirm previous results, suggesting that New Guinea landraces are principally derived from the Northern neotropical genepool (Camote and Batata lines, from the Caribbean and Central America). However, chloroplast data suggest that South American clones (early Kumara line clones or, more probably, later reintroductions) were also introduced into New Guinea and then recombined with existing genotypes. The frequency distribution of pairwise distances between New Guinea landraces suggests that sexual reproduction, rather than somaclonal variation, has played a predominant role in the diversification of sweet potato. The frequent incorporation of plants issued from true seed by farmers, and the geographical and cultural barriers constraining crop diffusion in this topographically and linguistically heterogeneous island, has led to the accumulation of an impressive number of variants. As the diversification of sweet potato in New Guinea is primarily the result of farmers' management of the reproductive biology of their crop, we argue that on-farm conservation programmes that implement distribution of core samples (clones representing the useful diversity of the species) and promote on-farm selection of locally adapted variants may allow local communities to fashion relatively autonomous strategies for coping with ongoing global change.

  2. Molecular cloning and characterization of a flavonoid 3'-hydroxylase gene from purple-fleshed sweet potato (Ipomoea batatas).

    Science.gov (United States)

    Zhou, Wei; Gong, Yifu; Lu, Xu; Huang, Chengtao; Gao, Feng

    2012-01-01

    Flavonoid 3'-hydroxylase (F3'H: EC 1.14.13.21) is an important enzyme which determines the hydroxylation pattern of anthocyanins. In this study, the full-length cDNA and genomic DNA of F3'H were isolated and characterized from the purple-fleshed sweet potato (Ipomoea batatas). IbF3''H was 1,789 bp containing a 1,554 bp open reading frame (ORF) encoding 518 amino acids. Comparative and bioinformatic analysis revealed that IbF3'H was highly homologous with F3'Hs from other plant species. Conserved domain search revealed that IbF3'H was a cytochrome P450 dependent enzyme. Three F3'H-specific motifs (V75VVAAS80, G427GEK430 and V433DVKG437) were conserved in IbF3'H. Phylogenetic analysis revealed that IbF3'H was clustered into the same subgroup with the homologues from I. purpurea, I. tricolor and I. nil. There were multiple copies of the IbF3'H gene in the genome of I. batatas. IbF3'H was constitutively expressed in all tested tissues including fibrous roots, thick roots, storage roots, stems and leaves. During storage root formation, IbF3'H was expressed most abundantly in the storage roots, suggesting that the anthocyanin biosynthesis is also active in the under-ground organs. IbF3'H expression was associated with anthocyanin accumulation in five different sweet potato cultivars tested. Complementative analysis implied that the full-length cDNA of IbF3'H could encode a functional protein and had a special catalytic activity of flavonoid 3'-hydroxylase.

  3. On the origin of sweet potato (Ipomoea batatas (L.) Lam.) genetic diversity in New Guinea, a secondary centre of diversity

    Science.gov (United States)

    Roullier, C; Kambouo, R; Paofa, J; McKey, D; Lebot, V

    2013-01-01

    New Guinea is considered the most important secondary centre of diversity for sweet potato (Ipomoea batatas). We analysed nuclear and chloroplast genetic diversity of 417 New Guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical America. The molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical America. Nuclear data confirm previous results, suggesting that New Guinea landraces are principally derived from the Northern neotropical genepool (Camote and Batata lines, from the Caribbean and Central America). However, chloroplast data suggest that South American clones (early Kumara line clones or, more probably, later reintroductions) were also introduced into New Guinea and then recombined with existing genotypes. The frequency distribution of pairwise distances between New Guinea landraces suggests that sexual reproduction, rather than somaclonal variation, has played a predominant role in the diversification of sweet potato. The frequent incorporation of plants issued from true seed by farmers, and the geographical and cultural barriers constraining crop diffusion in this topographically and linguistically heterogeneous island, has led to the accumulation of an impressive number of variants. As the diversification of sweet potato in New Guinea is primarily the result of farmers' management of the reproductive biology of their crop, we argue that on-farm conservation programmes that implement distribution of core samples (clones representing the useful diversity of the species) and promote on-farm selection of locally adapted variants may allow local communities to fashion relatively autonomous strategies for coping with ongoing global change. PMID:23531982

  4. Characterization of Acylated Anthocyanins in Callus Induced From Storage Root of Purple-Fleshed Sweet Potato, Ipomoea batatas L

    Science.gov (United States)

    Terahara, N.

    2004-01-01

    Four anthocyanins were isolated from a highly pigmented callus induced from the storage root of purple-fleshed sweet potato (Ipomoea batatas L) cultivar Ayamurasaki. The anthocyanins were respectively identified as cyanidin 3-O-(2-O-(6-O-(E)-caffeoyl-β-D-glucopyranosyl)-β-D-glucopyranoside) -5-O-β-D-glucopyranoside, cyanidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-caffeoyl-β-D-glucopyranoside)-5-O-β-D-glucopyranoside, cyanidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-p-coumaroyl-β-D-glucopyranoside)- 5-O-β-D-glucopyranoside, and peonidin 3-O-(2-O-(6-O-(E)-p -coumaroyl-β-D-glucopyranosyl)-6-O-(E)-p-coumaroyl-β-D-glucopyranoside)-5-O-β-D-glucopyranoside by chemical and spectroscopic analyses. These anthocyanins were examined with respect to the stability in neutral aqueous solution as well as the radical scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. These acylated anthocyanins exhibited both higher stability and higher DPPH radical scavenging activity than corresponding nonacylated cyanidin and peonidin 3-O-sophoroside-5-O-glucosides. PMID:15577190

  5. Occurrence of LINE, gypsy-like, and copia-like retrotransposons in the clonally propagated sweet potato (Ipomoea batatas L.).

    Science.gov (United States)

    Okpul, Tom; Harding, Robert M; Dieters, Mark J; Godwin, Ian D

    2011-07-01

    Retrotransposons are a class of transposable elements that represent a major fraction of the repetitive DNA of most eukaryotes. Their abundance stems from their expansive replication strategies. We screened and isolated sequence fragments of long terminal repeat (LTR), gypsy-like reverse transcriptase (rt) and gypsy-like envelope (env) domains, and two partial sequences of non-LTR retrotransposons, long interspersed element (LINE), in the clonally propagated allohexaploid sweet potato (Ipomoea batatas (L.) Lam.) genome. Using dot-blot hybridization, these elements were found to be present in the ~1597 Mb haploid sweet potato genome with copy numbers ranging from ~50 to ~4100 as observed in the partial LTR (IbLtr-1) and LINE (IbLi-1) sequences, respectively. The continuous clonal propagation of sweet potato may have contributed to such a multitude of copies of some of these genomic elements. Interestingly, the isolated gypsy-like env and gypsy-like rt sequence fragments, IbGy-1 (~2100 copies) and IbGy-2 (~540 copies), respectively, were found to be homologous to the Bagy-2 cDNA sequences of barley (Hordeum vulgare L.). Although the isolated partial sequences were found to be homologous to other transcriptionally active elements, future studies are required to determine whether they represent elements that are transcriptionally active under normal and (or) stressful conditions.

  6. Anti-Inflammatory and Anticancer Activities of Taiwanese Purple-Fleshed Sweet Potatoes (Ipomoea batatas L. Lam) Extracts.

    Science.gov (United States)

    Sugata, Marcelia; Lin, Chien-Yih; Shih, Yang-Chia

    2015-01-01

    Purple-fleshed sweet potato (PFSP) (Ipomoea batatas L. Lam) has been known to possess high amount of anthocyanins which contribute to its antioxidant activity. However, a few reports are available concerning its anti-inflammatory and anticancer properties. In this study, PFSP "Tainung 73," which is locally grown in Taiwan, was steamed and extracted using acidified ethanol pH 3.5 under 80°C. Two kinds of crude anthocyanins extracts were obtained, namely, SP (Steamed, Peeled) and SNP (Steamed, No Peeled). Then, anti-inflammatory and anticancer activities of these extracts were investigated. Cell viability assay (MTT) showed that SP and SNP extracts were not toxic to RAW 264.7 cells. They even exhibited anti-inflammatory activities by suppressing the production of NO and proinflammatory cytokines, such as NF-κβ, TNF-α, and IL-6, in LPS-induced macrophage cells. Anticancer activities of these extracts were displayed through their ability to inhibit the growth of cancer cell lines, such as MCF-7 (breast cancer), SNU-1 (gastric cancer), and WiDr (colon adenocarcinoma), in concentration- and time-dependent manner. Further studies also revealed that SP extracts could induce apoptosis in MCF-7 and SNU-1 cancer cells through extrinsic and intrinsic pathway. In the future, PSFP extracts may have potential to be applied in nutraceutical, pharmaceutical, and food industries.

  7. A dithiol glutaredoxin cDNA from sweet potato (Ipomoea batatas [L.] Lam): enzyme properties and kinetic studies.

    Science.gov (United States)

    Chi, X-W; Lin, C-T; Jiang, Y-C; Wen, L; Lin, C-T

    2012-07-01

    Glutaredoxins (Grx) play an important role in reduction of protein glutathione mixed disulphides. An IbGrx cDNA (561 bp, EF362614) encoding a putative dithiol Grx was cloned from sweet potato (Ipomoea batatas [L.] Lam). The deduced amino acid sequence is conserved among the reported dithiol Grx, having a CGYC dithiol motif at the active site. A 3-D structural model was created based on the known crystal structure of a poplar Grx (GrxC1). To characterise the IbGrx protein, the coding region was subcloned into an expression vector and transformed into Escherichia coli. The recombinant His(6) -tagged IbGrx was expressed and purified by metal affinity chromatography. The purified enzyme showed a monomeric band, as demonstrated with 15% SDS-PAGE. The Michaelis constant (K(M) ) for ß-hydroxyethyl disulphide (HED) was 0.50 ± 0.08 Mm. The enzyme retained 60% activity at 80 °C for 16 min. The enzyme was active over a broad pH range from 6.0 to 11.0, and in the presence of imidazole up to 0.4 M. The enzyme was susceptible to protease. © 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.

  8. Effect of high pressure on the saccharification of starch in the tuberous root of sweet potato (Ipomoea batatas).

    Science.gov (United States)

    Shigematsu, Toru; Furukawa, Naho; Takaoka, Ryo; Hayashi, Mayumi; Sasao, Shoji; Ueno, Shigeaki; Nakajima, Kanako; Kido, Miyuki; Nomura, Kazuki; Iguchi, Akinori

    2017-12-01

    We analyzed the effect of high hydrostatic pressure (HHP) treatment on reducing sugar production in the tuberous root of sweet potato (Ipomoea batatas), based on pressure-gelatinization of starch and subsequent saccharification by internal amylases. HHP treatment at up to 600MPa at ambient temperature for 10min did not apparently affect the reducing sugar concentration in tuberous root. However, HHP treatment at 100 to 500MPa and 60°C or 70°C for 10min increased reducing sugar concentration as both the pressure and temperature increased. The reducing sugar concentration after HHP treatment at 500MPa and 70°C for 10min was roughly comparable to that of the thermal treatment control (80°C for 10min under atmospheric pressure). HHP treatment enabled the gelatinization and enzymatic saccharification of starch in the tuberous root of sweet potato, at a lower temperature than required by thermal treatment at atmospheric pressure. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. The physicochemical properties of microwave-assisted encapsulated anthocyanins from Ipomoea batatas as affected by different wall materials

    Science.gov (United States)

    Mohd Nawi, Norazlina; Muhamad, Ida Idayu; Mohd Marsin, Aishah

    2015-01-01

    This study focuses on the impact of different wall materials on the physicochemical properties of microwave-assisted encapsulated anthocyanins from Ipomoea batatas. Using the powder characterization technique, purple sweet potato anthocyanin (PSPAs) powders were analysed for moisture content, water activity, dissolution time, hygroscopicity, color and morphology. PSPAs were produced using different wall materials: maltodextrin (MD), gum arabic (GA) and a combination of gum arabic and maltodextrin (GA + MD) at a 1:1 ratio. Each of the wall materials was homogenized to the core material at a core/wall material ratio of 5 and were microencapsulated by microwave-assisted drying at 1100 W. Results indicated that encapsulated powder with the GA and MD combination presented better quality of powder with the lowest value of moisture content and water activity. With respect to morphology, the microcapsule encapsulated with GA + MD showed several dents in coating surrounding its core material, whereas other encapsulated powders showed small or slight dents entrapped onto the bioactive compound. Colorimetric analysis showed changes in values of L, a*, b*, hue and chroma in the reconstituted powder compared to the initial powder. PMID:25838887

  10. The influence of deep frying using various vegetable oils on acrylamide formation in sweet potato (Ipomoea batatas L. Lam) chips.

    Science.gov (United States)

    Lim, P K; Jinap, S; Sanny, M; Tan, C P; Khatib, A

    2014-01-01

    The objective of this study was to evaluate the precursors of acrylamide formation in sweet potato (SP) (Ipomoea batatas L. Lam) chips and to determine the effect of different types of vegetable oils (VOs), that is, palm olein, coconut oil, canola oil, and soya bean oil, on acrylamide formation. The reducing sugars and amino acids in the SP slices were analyzed, and the acrylamide concentrations of SP chips were measured. SP chips that were fried in a lower degree of unsaturation oils contained a lower acrylamide concentration (1443 μg/kg), whereas those fried with higher degree of unsaturated oils contained a higher acrylamide concentration (2019 μg/kg). SP roots were found to contain acrylamide precursors, that is, 4.17 mg/g glucose and 5.05 mg/g fructose, and 1.63 mg/g free asparagine. The type of VO and condition used for frying, significantly influenced acrylamide formation. This study clearly indicates that the contribution of lipids in the formation of acrylamide should not be neglected. © 2013 Institute of Food Technologists®

  11. Antioxidant and prebiotic activity of five peonidin-based anthocyanins extracted from purple sweet potato (Ipomoea batatas (L.) Lam.).

    Science.gov (United States)

    Sun, Hanju; Zhang, Pingping; Zhu, Yongsheng; Lou, Qiuyan; He, Shudong

    2018-03-22

    Twelve kinds of anthocyanins from the Chinese purple sweet potato cultivar (Ipomoea batatas (L.) Lam.) were extracted and identified using LC-MS/MS, which had a high content of peonidin-based anthocyanins. Five peonidin-based anthocyanin monomers (P1, P2, P3, P4 and P5) were isolated by preparative liquid chromatography with structural analyses using an Impact II Q-TOF MS/MS. Then, the functional properties of the anthocyanin monomers, such as the antioxidant activities, proliferative effects on probiotics, and their inhibition on harmful bacteria in vitro, were investigated. The peonidin-based components in purple sweet potato anthocyanins (PSPAs) showed good properties regarding scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anions, and had good potential in reducing the total power activity and Fe 2+ chelating ability. While the order of the antioxidant abilities was as follows: P4 > P5 > P3 > P2 > P1 > PSPAs. Microbial cultivations showed that P1, P2, P3, P4, P5 and PSPAs could induce the proliferation of Bifidobacterium bifidum, Bifidobacterium adolescentis, Bifidobacterium infantis and Lactobacillus acidophilus, and they inhibited the growth of Staphylococcus aureus and Salmonella typhimurium, suggesting the anthocyanins might have prebiotic-like activity through the modulation of the intestinal microbiota. Our results indicate that peonidin-based anthocyanins could be further utilized in health foods and pharmaceutical developments.

  12. IbACP, a sixteen-amino-acid peptide isolated from Ipomoea batatas leaves, induces carcinoma cell apoptosis.

    Science.gov (United States)

    Chang, Vincent H-S; Yang, Diane H-A; Lin, Hsin-Hung; Pearce, Gregory; Ryan, Clarence A; Chen, Yu-Chi

    2013-09-01

    A 16-amino-acid peptide was isolated from the leaves of sweet potato. The peptide caused a rapid alkalinization response in tomato suspension culture media, a characteristic of defense peptides in plants. No post-translational modification was observed on the peptide according to MALDI-MS analysis. We have named the peptide Ipomoea batatas anti-cancer peptide (IbACP). IbACP also was shown with the ability to dose-dependently inhibit Panc-1, a pancreatic cancer line, cell proliferation. The morphological observations of the Panc-1 cells by phase contrast microscopy showed significant changes after treatment with IbACP. Moreover, caspase-3 and PARP [poly(ADP-ribose) polymerase] were activated by IbACP treatment, followed by cell death. An increase in the levels of cleaved caspase-3 and -9 was also detected by an immunoblot assay after treatment with IbACP. In addition, genomic DNA fragmentation and decreased cellular proliferation were induced when IbACP was supplied to the Panc-1 cells, further demonstrating its biological relevance. The combined data indicates that IbACP peptide may have an important role in the regulation of cellular proliferation by inducing and promoting apoptosis through the mitochondrial apoptotic pathway. This report also showed that IbACP peptide contains potent anti-cancer effects and may play an important role in herbal medicine development. Copyright © 2013 Elsevier Inc. All rights reserved.

  13. Utilization of ensiled sweet potato (Ipomoea batatas (L.) Lam.) leaves as a protein supplement in diets for growing pigs.

    Science.gov (United States)

    Van An, L; Hong, T T T; Ogle, B; Lindberg, J E

    2005-01-01

    Four diets were formulated with protein from fishmeal (FM), groundnut cake (GC), ensiled sweet potato (Ipomoea batatas L. (Lam.)) leaves (SP) or ensiled sweet potato leaves with lysine (SPL). In experiment 1, 24 crossbred (Large White x Mong Cai) growing pigs were allocated randomly by sex into four groups of six pigs and given one of four diets. Experiment 2 was conducted using 16 crossbred pigs (Large White x Mong Cai) at four farms. On each farm, pigs were allocated to two experimental groups. One group was fed the FM diet and the other group the SPL diet. In experiment 1, the daily live weight gains (DLWG) were significantly higher (p 0.05). The feed cost per kg live weight gain was lowest for the SP and SPL diets compared to the FM and GC diets. The results of experiment 2 show that there were no significant differences in feed intake, DLWG and FCR between the two diets (p > 0.05). In conclusion, sweet potato leaves can replace fishmeal and groundnut cake in traditional Vietnamese diets for growing pigs.

  14. Relationship between Processing Method and the Glycemic Indices of Ten Sweet Potato (Ipomoea batatas Cultivars Commonly Consumed in Jamaica

    Directory of Open Access Journals (Sweden)

    Perceval S. Bahado-Singh

    2011-01-01

    Full Text Available This study investigated the effect of different traditional cooking methods on glycemic index (GI and glycemic response of ten Sweet potato (Ipomoea batatas cultivars commonly eaten in Jamaica. Matured tubers were cooked by roasting, baking, frying, or boiling then immediately consumed by the ten nondiabetic test subjects (5 males and 5 females; mean age of 27 ± 2 years. The GI varied between 41 ± 5–93 ± 5 for the tubers studied. Samples prepared by boiling had the lowest GI (41 ± 5–50 ± 3, while those processed by baking (82 ± 3–94 ± 3 and roasting (79 ± 4–93 ± 2 had the highest GI values. The study indicates that the glycemic index of Jamaican sweet potatoes varies significantly with the method of preparation and to a lesser extent on intravarietal differences. Consumption of boiled sweet potatoes could minimize postprandial blood glucose spikes and therefore, may prove to be more efficacious in the management of type 2 diabetes mellitus.

  15. Anti-Inflammatory and Anticancer Activities of Taiwanese Purple-Fleshed Sweet Potatoes (Ipomoea batatas L. Lam) Extracts

    Science.gov (United States)

    Sugata, Marcelia; Lin, Chien-Yih; Shih, Yang-Chia

    2015-01-01

    Purple-fleshed sweet potato (PFSP) (Ipomoea batatas L. Lam) has been known to possess high amount of anthocyanins which contribute to its antioxidant activity. However, a few reports are available concerning its anti-inflammatory and anticancer properties. In this study, PFSP “Tainung 73,” which is locally grown in Taiwan, was steamed and extracted using acidified ethanol pH 3.5 under 80°C. Two kinds of crude anthocyanins extracts were obtained, namely, SP (Steamed, Peeled) and SNP (Steamed, No Peeled). Then, anti-inflammatory and anticancer activities of these extracts were investigated. Cell viability assay (MTT) showed that SP and SNP extracts were not toxic to RAW 264.7 cells. They even exhibited anti-inflammatory activities by suppressing the production of NO and proinflammatory cytokines, such as NF-κβ, TNF-α, and IL-6, in LPS-induced macrophage cells. Anticancer activities of these extracts were displayed through their ability to inhibit the growth of cancer cell lines, such as MCF-7 (breast cancer), SNU-1 (gastric cancer), and WiDr (colon adenocarcinoma), in concentration- and time-dependent manner. Further studies also revealed that SP extracts could induce apoptosis in MCF-7 and SNU-1 cancer cells through extrinsic and intrinsic pathway. In the future, PSFP extracts may have potential to be applied in nutraceutical, pharmaceutical, and food industries. PMID:26509161

  16. Relationship between Processing Method and the Glycemic Indices of Ten Sweet Potato (Ipomoea batatas) Cultivars Commonly Consumed in Jamaica

    Science.gov (United States)

    Bahado-Singh, Perceval S.; Riley, Cliff K.; Wheatley, Andrew O.; Lowe, Henry I. C.

    2011-01-01

    This study investigated the effect of different traditional cooking methods on glycemic index (GI) and glycemic response of ten Sweet potato (Ipomoea batatas) cultivars commonly eaten in Jamaica. Matured tubers were cooked by roasting, baking, frying, or boiling then immediately consumed by the ten nondiabetic test subjects (5 males and 5 females; mean age of 27 ± 2 years). The GI varied between 41 ± 5–93 ± 5 for the tubers studied. Samples prepared by boiling had the lowest GI (41 ± 5–50 ± 3), while those processed by baking (82 ± 3–94 ± 3) and roasting (79 ± 4–93 ± 2) had the highest GI values. The study indicates that the glycemic index of Jamaican sweet potatoes varies significantly with the method of preparation and to a lesser extent on intravarietal differences. Consumption of boiled sweet potatoes could minimize postprandial blood glucose spikes and therefore, may prove to be more efficacious in the management of type 2 diabetes mellitus. PMID:22132322

  17. Sweet potato (Ipomoea batatas L.) leaves suppressed oxidation of low density lipoprotein (LDL) in vitro and in human subjects

    Science.gov (United States)

    Nagai, Miu; Tani, Mariko; Kishimoto, Yoshimi; Iizuka, Maki; Saita, Emi; Toyozaki, Miku; Kamiya, Tomoyasu; Ikeguchi, Motoya; Kondo, Kazuo

    2011-01-01

    Sweet potato (Ipomoea batatas L.) leaves are consumed as vegetables around the world, especially in Southeast Asia. The aim of this study was to investigate the inhibitory effect of sweet potato leaves on low-density lipoprotein oxidation in vitro and in human subjects. We compared the antioxidant activity of 8 kinds of sweet potato leaves. Every sweet potato leaf had high radical scavenging activity and prolonged a lag time for starting low-density lipoprotein oxidation in vitro. We found that sweet potato leaves contained abundant polyphenol compounds and the radical scavenging activity and prolongation rate of lag time were highly correlated with total polyphenol content. We also confirmed that thiobarbituric acid reactive substances production was increased in endothelial cell-mediated low-density lipoprotein oxidation, which was decreased by treatment with sweet potato leaves. We further measured the low-density lipoprotein oxidizability in 13 healthy volunteers after their intake of 18 g of “Suioh”, raw sweet potato leaves. “Suioh” prolonged a lag time for starting low-density lipoprotein oxidation and decreased low-density lipoprotein mobility. These results suggest that sweet potato leaves have antioxidant activity leading to the suppression of low-density lipoprotein oxidation. PMID:21562639

  18. The physicochemical properties of microwave-assisted encapsulated anthocyanins from Ipomoea batatas as affected by different wall materials.

    Science.gov (United States)

    Mohd Nawi, Norazlina; Muhamad, Ida Idayu; Mohd Marsin, Aishah

    2015-03-01

    This study focuses on the impact of different wall materials on the physicochemical properties of microwave-assisted encapsulated anthocyanins from Ipomoea batatas. Using the powder characterization technique, purple sweet potato anthocyanin (PSPAs) powders were analysed for moisture content, water activity, dissolution time, hygroscopicity, color and morphology. PSPAs were produced using different wall materials: maltodextrin (MD), gum arabic (GA) and a combination of gum arabic and maltodextrin (GA + MD) at a 1:1 ratio. Each of the wall materials was homogenized to the core material at a core/wall material ratio of 5 and were microencapsulated by microwave-assisted drying at 1100 W. Results indicated that encapsulated powder with the GA and MD combination presented better quality of powder with the lowest value of moisture content and water activity. With respect to morphology, the microcapsule encapsulated with GA + MD showed several dents in coating surrounding its core material, whereas other encapsulated powders showed small or slight dents entrapped onto the bioactive compound. Colorimetric analysis showed changes in values of L, a*, b*, hue and chroma in the reconstituted powder compared to the initial powder.

  19. Sweet potato (Ipomoea batatas L.) leaves suppressed oxidation of low density lipoprotein (LDL) in vitro and in human subjects.

    Science.gov (United States)

    Nagai, Miu; Tani, Mariko; Kishimoto, Yoshimi; Iizuka, Maki; Saita, Emi; Toyozaki, Miku; Kamiya, Tomoyasu; Ikeguchi, Motoya; Kondo, Kazuo

    2011-05-01

    Sweet potato (Ipomoea batatas L.) leaves are consumed as vegetables around the world, especially in Southeast Asia. The aim of this study was to investigate the inhibitory effect of sweet potato leaves on low-density lipoprotein oxidation in vitro and in human subjects. We compared the antioxidant activity of 8 kinds of sweet potato leaves. Every sweet potato leaf had high radical scavenging activity and prolonged a lag time for starting low-density lipoprotein oxidation in vitro. We found that sweet potato leaves contained abundant polyphenol compounds and the radical scavenging activity and prolongation rate of lag time were highly correlated with total polyphenol content. We also confirmed that thiobarbituric acid reactive substances production was increased in endothelial cell-mediated low-density lipoprotein oxidation, which was decreased by treatment with sweet potato leaves. We further measured the low-density lipoprotein oxidizability in 13 healthy volunteers after their intake of 18 g of "Suioh", raw sweet potato leaves. "Suioh" prolonged a lag time for starting low-density lipoprotein oxidation and decreased low-density lipoprotein mobility. These results suggest that sweet potato leaves have antioxidant activity leading to the suppression of low-density lipoprotein oxidation.

  20. Effects of a killed-cover crop mulching system on sweetpotato production, soil pests, and insect predators in South Carolina.

    Science.gov (United States)

    Jackson, D Michael; Harrison, Howard F

    2008-12-01

    Sweetpotatoes, Ipomoea batatas (L.) Lam. (Convolvulaceae), are typically grown on bare soil where weeds and erosion can be serious problems. Conservation tillage systems using cover crop residues as mulch can help reduce these problems, but little is known about how conservation tillage affects yield and quality of sweetpotato or how these systems impact populations of beneficial and pest insects. Therefore, field experiments were conducted at the U.S. Vegetable Laboratory, Charleston, SC, in 2002-2004 to evaluate production of sweetpotatoes in conventional tillage versus a conservation tillage system by using an oat (Avena sativa L. (Poaceae)-crimson clover (Trifolium incarnatum L.) (Fabaceae) killed-cover crop (KCC) mulch. The four main treatments were 1) conventional tillage, hand-weeded; 2) KCC, hand-weeded; 3) conventional tillage, weedy; and 4) KCC, weedy. Each main plot was divided into three subplots, whose treatments were sweetpotato genotypes: 'Ruddy', which is resistant to soil insect pests; and 'SC1149-19' and 'Beauregard', which are susceptible to soil insect pests. For both the KCC and conventional tillage systems, sweetpotato yields were higher in plots that received hand weeding than in weedy plots. Orthogonal contrasts revealed a significant effect of tillage treatment (conventional tillage versus KCC) on yield in two of the 3 yr. Ruddy remained resistant to injury by soil insect pests in both cropping systems; and it consistently had significantly higher percentages of clean roots and less damage by wireworm-Diabrotica-Systena complex, sweetpotato flea beetles, grubs, and sweetpotato weevils than the two susceptible genotypes. In general, injury to sweetpotato roots by soil insect pests was not significantly higher in the KCC plots than in the conventionally tilled plots. Also, more fire ants, rove beetles, and carabid beetle were captured by pitfall traps in the KCC plots than in the conventional tillage plots during at least 1 yr of the study

  1. Application of IBA in environmental remediation using skins of cocoa (Theobroma cacao) and sweet potatoes (Ipomoea batatas)

    Energy Technology Data Exchange (ETDEWEB)

    Sumbu, K. [Department of Chemistry, Cape Peninsula University of Technology, PO Box 1906, Bellville, 7535 (South Africa); Mars, J.A.; Gihwala, D. [Department of Biomedical Sciences, Cape Peninsula University of Technology, PO Box 1906, Bellville, 7535 (South Africa)

    2013-07-01

    Full text: The industrial sector is one of the most dynamic sectors of the economy and is of cardinal importance in economic development and poverty alleviation. Furthermore, economies with low levels of industrialisation are gradually shifting dependence from agriculture to the industrial sector, while developed economies, with a high level of industrialisation, are shifting from the industrial to the service sector [1]. Amongst the various industrial sectors, a substantial portion of effluents containing heavy metals are generated by electroplating (nickel, lead, zinc and copper), tanneries (chrome) and other chemical industries. Many engineering processes have been designed to remove the heavy metals from the polluted parts of the ecosystem. These processes are however highly capital intensive. To establish a less costly means of removal of heavy metal pollution, the dried skins of cocoa (Theobroma cacao) and sweet potato (Ipomoea batatas) were pulverized and used in the absorption of the heavy metals Ni and Pb. After adsorption by the metals, the powders were dried and then pressed into tablets. For quantification of the elemental adsorption, PIXE, using the Geo PIXE computational software, is a versatile multi-element analytical technique, and has minimum detection limits down to concentration ranges as low as 0.1 parts per million [2,3]. To determine the composition of the major elements such as C, O and N, Backscattering Spectrometry, using SimNRA software, [4] was used. In this study we report on the economic viability of using the skins of cocoa and sweet potato in the removal of Ni and Pd from polluted waters. (author)

  2. Elevated compartmentalization of Na+ into vacuoles improves salt and cold stress tolerance in sweet potato (Ipomoea batatas).

    Science.gov (United States)

    Fan, Weijuan; Deng, Gaifang; Wang, Hongxia; Zhang, Hongxia; Zhang, Peng

    2015-08-01

    Salinity and low temperature are the main limiting factors for sweet potato (Ipomoea batatas) growth and agricultural productivity. Various studies have shown that plant NHX-type antiporter plays a crucial role in regulating plant tolerance to salt stress by intracellular Na(+) compartmentalization. The Arabidopsis thaliana AtNHX1 gene that encodes a vacuolar Na(+) /H(+) antiporter was introduced into the sweet potato cultivar Xushu-22 by Agrobacterium-mediated transformation to confer abiotic stress tolerance. Stable insertion of AtNHX1 into the sweet potato genome and its expression was confirmed by Southern blot and reverse transcription-polymerase chain reaction (RT-PCR). A remarkably higher Na(+) /H(+) exchange activity of tonoplast membrane from transgenic sweet potato lines (NOE) in comparison with wild-type (WT) plants confirmed the vacuolar antiporter function in mediating Na(+) /H(+) exchange. Under salt stress, NOE plants accumulated higher Na(+) and K(+) levels in their tissues compared with WT plants, maintaining high K(+) /Na(+) ratios. Consequently, NOE plants showed enhanced protection against cell damage due to the increased proline accumulation, preserved cell membrane integrity, enhanced reactive oxygen species (ROS) scavenging (e.g. increased superoxide dismutase activity), and reduced H2 O2 and malondialdehyde (MDA) production. Moreover, the transgenic plants showed improved cold tolerance through multiple mechanisms of action, revealing the first molecular evidence for NHX1 function in cold response. The transgenic plants showed better biomass production and root yield under stressful conditions. These findings demonstrate that overexpressing AtNHX1 in sweet potato renders the crop tolerant to both salt and cold stresses, providing a greater capacity for the use of AtNHX1 in improving crop performance under combined abiotic stress conditions. © 2014 Scandinavian Plant Physiology Society.

  3. Digital gene expression analysis based on integrated de novo transcriptome assembly of sweet potato [Ipomoea batatas (L.) Lam].

    Science.gov (United States)

    Tao, Xiang; Gu, Ying-Hong; Wang, Hai-Yan; Zheng, Wen; Li, Xiao; Zhao, Chuan-Wu; Zhang, Yi-Zheng

    2012-01-01

    Sweet potato (Ipomoea batatas L. [Lam.]) ranks among the top six most important food crops in the world. It is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. However, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. Hence, studies based on high-throughput sequencing technologies are needed to get a comprehensive and integrated genomic resource and better understanding of gene expression patterns in different tissues and at various developmental stages. Illumina paired-end (PE) RNA-Sequencing was performed, and generated 48.7 million of 75 bp PE reads. These reads were de novo assembled into 128,052 transcripts (≥ 100 bp), which correspond to 41.1 million base pairs, by using a combined assembly strategy. Transcripts were annotated by Blast2GO and 51,763 transcripts got BLASTX hits, in which 39,677 transcripts have GO terms and 14,117 have ECs that are associated with 147 KEGG pathways. Furthermore, transcriptome differences of seven tissues were analyzed by using Illumina digital gene expression (DGE) tag profiling and numerous differentially and specifically expressed transcripts were identified. Moreover, the expression characteristics of genes involved in viral genomes, starch metabolism and potential stress tolerance and insect resistance were also identified. The combined de novo transcriptome assembly strategy can be applied to other organisms whose reference genomes are not available. The data provided here represent the most comprehensive and integrated genomic resources for cloning and identifying genes of interest in sweet potato. Characterization of sweet potato transcriptome provides an effective tool for better understanding the molecular mechanisms of cellular processes including development of leaves and storage roots, tissue-specific gene expression, potential biotic and abiotic stress response in sweet

  4. Improved tolerance to various abiotic stresses in transgenic sweet potato (Ipomoea batatas) expressing spinach betaine aldehyde dehydrogenase.

    Science.gov (United States)

    Fan, Weijuan; Zhang, Min; Zhang, Hongxia; Zhang, Peng

    2012-01-01

    Abiotic stresses are critical delimiters for the increased productivity and cultivation expansion of sweet potato (Ipomoea batatas), a root crop with worldwide importance. The increased production of glycine betaine (GB) improves plant tolerance to various abiotic stresses without strong phenotypic changes, providing a feasible approach to improve stable yield production under unfavorable conditions. The gene encoding betaine aldehyde dehydrogenase (BADH) is involved in the biosynthesis of GB in plants, and the accumulation of GB by the heterologous overexpression of BADH improves abiotic stress tolerance in plants. This study is to improve sweet potato, a GB accumulator, resistant to multiple abiotic stresses by promoted GB biosynthesis. A chloroplastic BADH gene from Spinacia oleracea (SoBADH) was introduced into the sweet potato cultivar Sushu-2 via Agrobacterium-mediated transformation. The overexpression of SoBADH in the transgenic sweet potato improved tolerance to various abiotic stresses, including salt, oxidative stress, and low temperature. The increased BADH activity and GB accumulation in the transgenic plant lines under normal and multiple environmental stresses resulted in increased protection against cell damage through the maintenance of cell membrane integrity, stronger photosynthetic activity, reduced reactive oxygen species (ROS) production, and induction or activation of ROS scavenging by the increased activity of free radical-scavenging enzymes. The increased proline accumulation and systemic upregulation of many ROS-scavenging genes in stress-treated transgenic plants also indicated that GB accumulation might stimulate the ROS-scavenging system and proline biosynthesis via an integrative mechanism. This study demonstrates that the enhancement of GB biosynthesis in sweet potato is an effective and feasible approach to improve its tolerance to multiple abiotic stresses without causing phenotypic defects. This strategy for trait improvement in

  5. Influence of diesel contamination in soil on growth and dry matter partitioning of Lactuca sativa and Ipomoea batatas.

    Science.gov (United States)

    Fatokun, Kayode; Zharare, Godfrey Elijah

    2015-09-01

    Phytotoxic effect of diesel contaminated soil was investigated on growth and dry matter partitioning in Lactuca sativa and Ipomoea batatas in greenhouse pot experiment at two concentration range (0-30 ml and 0-6 ml diesel kg(-1) soil) for 14 weeks. The results indicated thatwhole plant biomass, stem length, root length, number of leaves and leaf chlorophyll in two plants were negatively correlated with increasing diesel concentrations. The critical concentration of diesel associated with 10% decrease in plant growth was 0.33 ml for lettuce and 1.50 ml for sweet potato. Thus, growth of lettuce in diesel contaminated soil was more sensitive than sweet potato. The pattern of dry matter partitioning between root and shoot in both plants were similar. In 0-6 ml diesel contamination range, allocation of dry matter to shoot system was favoured resulting in high shoot: root ratio of 4.54 and 12.91 for lettuce and sweet potato respectively. However, in 0-30 ml diesel contamination range, allocation of dry matter to root was favoured, which may have been an adaptive mechanism in which the root system was used for storage in addition to increasing the capacity for foraging for mineral nutrients and water. Although lettuce accumulated more metals in its tissue than sweet potato, the tissue mineral nutrients in both species did not vary to great extent. The critical diesel concentration for toxicity suggested that the cause of mortality and poor growth of sweet potato and lettuce grown in diesel contaminated soil was due to presence of hydrocarbons in diesel.

  6. Efficient embryogenic suspension culturing and rapid transformation of a range of elite genotypes of sweet potato (Ipomoea batatas [L.] Lam.).

    Science.gov (United States)

    Yang, Jun; Bi, Hui-Ping; Fan, Wei-Juan; Zhang, Min; Wang, Hong-Xia; Zhang, Peng

    2011-12-01

    Efficient Agrobacterium tumefaciens-mediated transformation was developed using embryogenic suspension cell cultures of elite sweet potato (Ipomoea batatas [L.] Lam.) cultivars, including Ayamurasaki, Sushu2, Sushu9, Sushu11, Wanshu1, Xushu18 and Xushu22. Embryogenic suspension cultures were established in LCP medium using embryogenic calli induced from apical or axillary buds on an induction medium containing 2 mg l(-1) 2,4-D. Suspension cultures were co-cultivated with A. tumefaciens strain LBA4404 harboring the binary plasmid pCAMBIA1301 with the hpt gene as a selectable marker and an intron-interrupted uidA gene as a visible marker. Several key steps of the sweet potato transformation system have been investigated and optimized, including the appropriate antibiotics and their concentrations for suppressing Agrobacterium growth and the optimal doses of hygromycin for transformant selection. A total of 485 putative transgenic plant lines were produced from the transformed calli via somatic embryogenesis and germination to plants under 10 mg l(-1) hygromycin and 200 mg l(-1) cefotaxime. PCR, GUS and Southern blot analyses of the regenerated plants showed that 92.35% of them were transgenic. The number of T-DNA insertions varied from one to three in most transgenic plant lines. Plants showed 100% survival when 308 transgenics were transferred to soil in the greenhouse and then to the field. Most of them were morphologically normal, with the production of storage roots after 3 months of cultivation in the greenhouse or fields. The development of such a robust transformation method suitable to a range of sweet potato genotypes not only provides a routine tool for genetic improvement via transgenesis but also allows us to conduct a functional verification of endogenous genes in sweet potato. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  7. Sweet potato Ipomoea Batatas Modulates Radiation-induced Oxidative damage in Liver and kidney of Male Albino Rats

    International Nuclear Information System (INIS)

    Darwish, M. M.; Farag, M. F. S.; Osman, N. N.

    2010-01-01

    Sweet potato Ipomoea Batatas, one of the major vegetable crops consumed worldwide, is rich in phytochemicals, which displayed antioxidant activities. This work aims at assessing the radio-protective properties of sweet potato tubers on liver and kidney tissues. Male albino rats were whole body exposed to 0.5 Gy day after day for a period of 20 days. Animal received orally prepared aqueous extract of sweet potato tubers (100 mg kg/body weight), one week before irradiation and during the period of radiation exposure. The results demonstrated that irradiation of rats induced a significant increase in lipid peroxides level measured as thiobarbituric acid reactive substances (TBARS) concomitant with a significant decrease in superoxide dismutase (SOD), and catalase (CAT) activity and glutathione (GSH) content in liver and kidney tissues. Administration of a freshly prepared aqueous extract of sweet potato tubers to rats, one week pre-irradiation and during the period of radiation exposure has significantly of ameliorated the oxidative stress in both tissues. The significant amelioration in oxidative stress was substantiated by improvement of liver and kidney enzymes Treatment of rats with sweet potato has significantly reduced the increase in serum alanine amino transferase (ALT), aspartate amino transferase (AST) and lactate dehydrogenase (LDH) activity, serum creatinine and urea levels. Furthermore, hyperglycemia and alteration in lipid profile manifested by a significant increase in triglycerides (TG), total cholesterol (TC), and low density lipoprotein cholesterol (LDL-C), and a significant decrease in high density lipoprotein cholesterol (HDL-C), were improved in sweet potato-treated irradiated rats compared to those only irradiated. According to the results obtained in the present study, it could be concluded that sweet potato through its antioxidant activities could protect cellular membrane from radiation induced oxidative damage in animals and preserve the

  8. Application of IBA in environmental remediation using skins of cocoa (Theobroma cacao) and sweet potatoes (Ipomoea batatas)

    International Nuclear Information System (INIS)

    Sumbu, K.; Mars, J.A.; Gihwala, D.

    2013-01-01

    Full text: The industrial sector is one of the most dynamic sectors of the economy and is of cardinal importance in economic development and poverty alleviation. Furthermore, economies with low levels of industrialisation are gradually shifting dependence from agriculture to the industrial sector, while developed economies, with a high level of industrialisation, are shifting from the industrial to the service sector [1]. Amongst the various industrial sectors, a substantial portion of effluents containing heavy metals are generated by electroplating (nickel, lead, zinc and copper), tanneries (chrome) and other chemical industries. Many engineering processes have been designed to remove the heavy metals from the polluted parts of the ecosystem. These processes are however highly capital intensive. To establish a less costly means of removal of heavy metal pollution, the dried skins of cocoa (Theobroma cacao) and sweet potato (Ipomoea batatas) were pulverized and used in the absorption of the heavy metals Ni and Pb. After adsorption by the metals, the powders were dried and then pressed into tablets. For quantification of the elemental adsorption, PIXE, using the Geo PIXE computational software, is a versatile multi-element analytical technique, and has minimum detection limits down to concentration ranges as low as 0.1 parts per million [2,3]. To determine the composition of the major elements such as C, O and N, Backscattering Spectrometry, using SimNRA software, [4] was used. In this study we report on the economic viability of using the skins of cocoa and sweet potato in the removal of Ni and Pd from polluted waters. (author)

  9. Syk - GTP RAC-1 mediated immune-stimulatory effect of Cuscuta epithymum, Ipomoea batata and Euphorbia hirta plant extracts.

    Science.gov (United States)

    Sudam, Vanitha Sagar; Potnuri, Ajay Godwin; Subhashini, N J Prameela

    2017-12-01

    Polymorphonuclear neutrophils (PMNn) are the pivotal mediators of phagocytosis. In addition to neutropenia, impaired neutrophilic function is associated with pathological conditions and immuno-deficiencies. Henceforth, Immuno-stimulatory strategies targeting neutrophilic function are indeed powerful tools in combating obstinate infections. In appreciation towards the usefulness of herbal medicines in therapeutic scenario, the present study was carried out to analyse the immuno-stimulatory effect of Cuscuta epithymum, Ipomoea batata and Euphorbia hirta using in-vitro and in-vivo rodent experimental models. Throughout the experimentation, phagocytosis was studied and expressed as phagocytotic index and percentage phagocytosis. Different extracts of these plants were initially screened for their potency to induce phagocytosis in PMNn and the methanolic fractions, which are effective, were considered for further experimentation.The phagocytosis stimulation by the methanolic extracts was compared with the standard Granulocyte Macrophage - Colony Stimulating Factor (GM-CSF) at a dose of 65ng/ml. Immunoblotting analysis shown that the methanolic extracts induce the phosphorylation of Syk which in turn phosphorylates GDP-RAC-1, hinting the possible mechanism of action. Following these in vitro investigations, the potency of methanolic extracts was assessed using rat model by performing carbon clearance assay, Delayed Type Hypersensitivity and antibody titre.The phosphorylation status of Syk and GDP-RAC-1 was also assessed in the edematous fluid collected from the right hind paw. In vivo findings were in agreement with the in vitro findings by presenting an improved immune response and increased phosphorylation of Syk and GDP-RAC-1. Conclusively, this study provides the initial insights into the therapeutic implications of the tropical plants in inducing phagocytosis. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  10. Digital Gene Expression Analysis Based on Integrated De Novo Transcriptome Assembly of Sweet Potato [Ipomoea batatas (L.) Lam.

    Science.gov (United States)

    Wang, Hai-Yan; Zheng, Wen; Li, Xiao; Zhao, Chuan-Wu; Zhang, Yi-Zheng

    2012-01-01

    Background Sweet potato (Ipomoea batatas L. [Lam.]) ranks among the top six most important food crops in the world. It is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. However, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. Hence, studies based on high-throughput sequencing technologies are needed to get a comprehensive and integrated genomic resource and better understanding of gene expression patterns in different tissues and at various developmental stages. Methodology/Principal Findings Illumina paired-end (PE) RNA-Sequencing was performed, and generated 48.7 million of 75 bp PE reads. These reads were de novo assembled into 128,052 transcripts (≥100 bp), which correspond to 41.1 million base pairs, by using a combined assembly strategy. Transcripts were annotated by Blast2GO and 51,763 transcripts got BLASTX hits, in which 39,677 transcripts have GO terms and 14,117 have ECs that are associated with 147 KEGG pathways. Furthermore, transcriptome differences of seven tissues were analyzed by using Illumina digital gene expression (DGE) tag profiling and numerous differentially and specifically expressed transcripts were identified. Moreover, the expression characteristics of genes involved in viral genomes, starch metabolism and potential stress tolerance and insect resistance were also identified. Conclusions/Significance The combined de novo transcriptome assembly strategy can be applied to other organisms whose reference genomes are not available. The data provided here represent the most comprehensive and integrated genomic resources for cloning and identifying genes of interest in sweet potato. Characterization of sweet potato transcriptome provides an effective tool for better understanding the molecular mechanisms of cellular processes including development of leaves and storage roots, tissue-specific gene

  11. Improved Tolerance to Various Abiotic Stresses in Transgenic Sweet Potato (Ipomoea batatas) Expressing Spinach Betaine Aldehyde Dehydrogenase

    Science.gov (United States)

    Fan, Weijuan; Zhang, Min; Zhang, Hongxia; Zhang, Peng

    2012-01-01

    Abiotic stresses are critical delimiters for the increased productivity and cultivation expansion of sweet potato (Ipomoea batatas), a root crop with worldwide importance. The increased production of glycine betaine (GB) improves plant tolerance to various abiotic stresses without strong phenotypic changes, providing a feasible approach to improve stable yield production under unfavorable conditions. The gene encoding betaine aldehyde dehydrogenase (BADH) is involved in the biosynthesis of GB in plants, and the accumulation of GB by the heterologous overexpression of BADH improves abiotic stress tolerance in plants. This study is to improve sweet potato, a GB accumulator, resistant to multiple abiotic stresses by promoted GB biosynthesis. A chloroplastic BADH gene from Spinacia oleracea (SoBADH) was introduced into the sweet potato cultivar Sushu-2 via Agrobacterium-mediated transformation. The overexpression of SoBADH in the transgenic sweet potato improved tolerance to various abiotic stresses, including salt, oxidative stress, and low temperature. The increased BADH activity and GB accumulation in the transgenic plant lines under normal and multiple environmental stresses resulted in increased protection against cell damage through the maintenance of cell membrane integrity, stronger photosynthetic activity, reduced reactive oxygen species (ROS) production, and induction or activation of ROS scavenging by the increased activity of free radical-scavenging enzymes. The increased proline accumulation and systemic upregulation of many ROS-scavenging genes in stress-treated transgenic plants also indicated that GB accumulation might stimulate the ROS-scavenging system and proline biosynthesis via an integrative mechanism. This study demonstrates that the enhancement of GB biosynthesis in sweet potato is an effective and feasible approach to improve its tolerance to multiple abiotic stresses without causing phenotypic defects. This strategy for trait improvement in

  12. H+ -pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.].

    Science.gov (United States)

    Fan, Weijuan; Wang, Hongxia; Wu, Yinliang; Yang, Nan; Yang, Jun; Zhang, Peng

    2017-06-01

    Iron (Fe) deficiency is one of the most common micronutrient deficiencies limiting crop production globally, especially in arid regions because of decreased availability of iron in alkaline soils. Sweet potato [Ipomoea batatas (L.) Lam.] grows well in arid regions and is tolerant to Fe deficiency. Here, we report that the transcription of type I H + -pyrophosphatase (H + -PPase) gene IbVP1 in sweet potato plants was strongly induced by Fe deficiency and auxin in hydroponics, improving Fe acquisition via increased rhizosphere acidification and auxin regulation. When overexpressed, transgenic plants show higher pyrophosphate hydrolysis and plasma membrane H + -ATPase activity compared with the wild type, leading to increased rhizosphere acidification. The IbVP1-overexpressing plants showed better growth, including enlarged root systems, under Fe-sufficient or Fe-deficient conditions. Increased ferric precipitation and ferric chelate reductase activity in the roots of transgenic lines indicate improved iron uptake, which is also confirmed by increased Fe content and up-regulation of Fe uptake genes, e.g. FRO2, IRT1 and FIT. Carbohydrate metabolism is significantly affected in the transgenic lines, showing increased sugar and starch content associated with the increased expression of AGPase and SUT1 genes and the decrease in β-amylase gene expression. Improved antioxidant capacities were also detected in the transgenic plants, which showed reduced H 2 O 2 accumulation associated with up-regulated ROS-scavenging activity. Therefore, H + -PPase plays a key role in the response to Fe deficiency by sweet potato and effectively improves the Fe acquisition by overexpressing IbVP1 in crops cultivated in micronutrient-deficient soils. © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  13. Digital gene expression analysis based on integrated de novo transcriptome assembly of sweet potato [Ipomoea batatas (L. Lam].

    Directory of Open Access Journals (Sweden)

    Xiang Tao

    Full Text Available BACKGROUND: Sweet potato (Ipomoea batatas L. [Lam.] ranks among the top six most important food crops in the world. It is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. However, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. Hence, studies based on high-throughput sequencing technologies are needed to get a comprehensive and integrated genomic resource and better understanding of gene expression patterns in different tissues and at various developmental stages. METHODOLOGY/PRINCIPAL FINDINGS: Illumina paired-end (PE RNA-Sequencing was performed, and generated 48.7 million of 75 bp PE reads. These reads were de novo assembled into 128,052 transcripts (≥ 100 bp, which correspond to 41.1 million base pairs, by using a combined assembly strategy. Transcripts were annotated by Blast2GO and 51,763 transcripts got BLASTX hits, in which 39,677 transcripts have GO terms and 14,117 have ECs that are associated with 147 KEGG pathways. Furthermore, transcriptome differences of seven tissues were analyzed by using Illumina digital gene expression (DGE tag profiling and numerous differentially and specifically expressed transcripts were identified. Moreover, the expression characteristics of genes involved in viral genomes, starch metabolism and potential stress tolerance and insect resistance were also identified. CONCLUSIONS/SIGNIFICANCE: The combined de novo transcriptome assembly strategy can be applied to other organisms whose reference genomes are not available. The data provided here represent the most comprehensive and integrated genomic resources for cloning and identifying genes of interest in sweet potato. Characterization of sweet potato transcriptome provides an effective tool for better understanding the molecular mechanisms of cellular processes including development of leaves and storage roots

  14. Improved tolerance to various abiotic stresses in transgenic sweet potato (Ipomoea batatas expressing spinach betaine aldehyde dehydrogenase.

    Directory of Open Access Journals (Sweden)

    Weijuan Fan

    Full Text Available Abiotic stresses are critical delimiters for the increased productivity and cultivation expansion of sweet potato (Ipomoea batatas, a root crop with worldwide importance. The increased production of glycine betaine (GB improves plant tolerance to various abiotic stresses without strong phenotypic changes, providing a feasible approach to improve stable yield production under unfavorable conditions. The gene encoding betaine aldehyde dehydrogenase (BADH is involved in the biosynthesis of GB in plants, and the accumulation of GB by the heterologous overexpression of BADH improves abiotic stress tolerance in plants. This study is to improve sweet potato, a GB accumulator, resistant to multiple abiotic stresses by promoted GB biosynthesis. A chloroplastic BADH gene from Spinacia oleracea (SoBADH was introduced into the sweet potato cultivar Sushu-2 via Agrobacterium-mediated transformation. The overexpression of SoBADH in the transgenic sweet potato improved tolerance to various abiotic stresses, including salt, oxidative stress, and low temperature. The increased BADH activity and GB accumulation in the transgenic plant lines under normal and multiple environmental stresses resulted in increased protection against cell damage through the maintenance of cell membrane integrity, stronger photosynthetic activity, reduced reactive oxygen species (ROS production, and induction or activation of ROS scavenging by the increased activity of free radical-scavenging enzymes. The increased proline accumulation and systemic upregulation of many ROS-scavenging genes in stress-treated transgenic plants also indicated that GB accumulation might stimulate the ROS-scavenging system and proline biosynthesis via an integrative mechanism. This study demonstrates that the enhancement of GB biosynthesis in sweet potato is an effective and feasible approach to improve its tolerance to multiple abiotic stresses without causing phenotypic defects. This strategy for trait

  15. KAJIAN PEMANFAATAN KULIT UBI JALAR UNGU (Ipomoea batatas L TERFERMENTASI DALAM RANSUM TERHADAP KONSUMSI DAN NUTRISI RANSUM DAN EFISIENSI PENGGUNAAN RANSUM PADA ITIK BALI UMUR 22 MINGGU

    Directory of Open Access Journals (Sweden)

    Belawa Yadnya T. G.

    2016-01-01

    Full Text Available Penelitian ini dilaksanakan bertujuan untuk mengetahui pengaruh pemanfaatan kulit ubi jalar ungu (Ipomoea batatas L terfermentasi dalam ransum terhadap konsumsi ransun dan nutrisi dan efisoensi penggunaan ransum pada itik Bali, umur 22 minggu. Menggunakan rancangan acak lengkap (RAL dengan lima perlakuan yang terdiri atas ransum A (ransum tanpa mengandung ubi jalar ungu, ransum B mengandung 10% kulit ubi jalar ungu, ransum C mengandung 10% kulit ubi jalar ungu terfermentasi, ransum D mengandung 20% kulit ubi jalar ungu, dan ransum E mengandung 20% kulit ubi jalar ungu terfermentasi. Setiap perlakuan terdiri atas empat ulangan dan setiap ungan terdiri atas lima ekor itik. Variabel yang diamati meliputi konsumsi ransum, konsumsi protein serat kasar dan antosianin dan kapasitas antioksidan ransum, serta efisiensi penggunaan ransum. Hasi penelitian menunjukkan bahwa pemberian ransum B, C, D dan E mengkonsumsi ransum, protein dan serat kasar yang lebih rendah (P<0,05, dan dapat meningkatkan konsumsi antosianin dan kapasitas antioksidan ransum (P<0,05 dibandingkan dengan perlakuan A. Pemberian kulit ubi jalar ungu fermentasi atau tanpa fermentasi dalam ransum dapat meningkatkan pertambahan bobot badan (P<0,05, sedangkan pada FCR terjadi penurunan secara nyata (0,05 dibandingkan dengan pemberian perlakuan A.Dari hasil penelitian ini dapat disimpulkan bahwa pemberian kulit ubi jalar ungu (Ipomoea batatas L dapat mengurangi konsumsi ransu, protein dan serat kasar serta dapat meningkatkan efisiensi penggunaan ransum.

  16. Evaluation sensorielle du couscous de farine de manioc (Manihot esculenta, Crantz substituée par celle de patate douce (Ipomoea batatas, Lam

    Directory of Open Access Journals (Sweden)

    Amadou, NM.

    2016-01-01

    Full Text Available Consumer Acceptance of Cassava (Manihot esculenta, Crantz flour's fufu substituted by Sweet Potato (Ipomoea batatas, Lam Flour. This study investigated the consumer acceptance of fufu made by substituting cassava (Manihot esculenta, Crantz flour with sweet potato (Ipomoea batatas, Lam flour at the Food Technology and Post-harvest laboratory of IRAD in Bambui, Cameroon. Four samples of Fufu flour samples F0, F1, F2 and F3 obtained by substituting cassava flour with 0%, 20%, 40% and 50% potato flour respectively were compared. A panel of 30 persons (habitual consumers of Cassava fufu flour carried out a sensory evaluation of the four samples and gave their level of appreciation with regards to taste, aroma, colour, and texture. The study showed that, the substitution of cassava flour with sweet potato flour had no effect on the aroma of the fufu. The sample containing 20% of sweet potato flour (F1 showed no significant difference in texture and aroma compared (p>0.05 to the control (F0. It was also considered as having the best taste (p<0.05 and was the sample preferred by the panelists. In conclusion, an increase in the proportion of sweet potato flour in the cassava flour had a negative influence on the colour, texture, taste and on the acceptance of the fufu.

  17. Efeito do glutaraldeído na adsorção de extrato proteico da Ipomoea Batatas (L Lam na superficie de uma zeólita analcima Effect of the glutaraldeide on the adsorption of proteic extract of the Ipomoea Batatas (L Lam in analcime zeolite surface

    Directory of Open Access Journals (Sweden)

    A. C. S. Gondim

    2005-09-01

    Full Text Available A zeólita analcime (Na16Al16Si32O96.nH 2O foi submetida a ataque ácido com HCl 0,25 mol.L-1, seguido da funcionalização com glutaraldeído, foi imersa em extrato protéico obtido da polpa da batata-doce (Ipomoea Batatas (L Lam, que tem em sua composição a enzima polifenol oxidase (PFO. As amostras foram submetidas a procedimentos de caracterização por microscopia eletrônica de varredura, espectroscopia na região do infravermelho com transformada de Fourier e termogravimetria.The analcime zeolite (Na16Al16Si32O96.nH2O was treated with hydrochloric acid 0.25 mol.L-1, followed by the funcionalization with glutaraldehyde and immersed in the proteic extract obtained from gross extract of sweet potato pulp (Ipomoea Batatas (L Lam, which presents in its composition the polyphenol oxidase enzyme (PPO. Other samples were submitted to the same procedure with characterizations by scanning electron microscopy, Fourier Transform Infrared spectroscopy and thermogravimetry.

  18. Evaluación agronómica de diez clones promisorios CIP y dos materiales nativos de Ipomoea batatas L.

    Directory of Open Access Journals (Sweden)

    Tique José

    2009-08-01

    Full Text Available

    Con el propósito de rescatar la riqueza genética de la batata (Ipomoea batatas, durante dos ciclos de cultivo se realizaron evaluaciones agronómicas de dos materiales nativos, en comparación con diez clones provenientes del Centro Internacional de la Papa, clasificados allí como promisorios. El trabajo se llevó a cabo en el municipio de Coyaima, en el departamento del Tolima (Colombia, con la participación de los integrantes del Cabildo Indígena Autónomo Independiente Buenavista, y la colaboración del Consorcio Latinoamericano y del Caribe de Apoyo a la Investigación y el Desarrollo de la Yuca. Los materiales nativos se colectaron en parcelas de agricultores y se clasificaron como ‘Bonanza’ y ‘Sangretoro’, nombres tradicionales dados por los indígenas de la zona. El experimento fue desarrollado a través de un diseño de bloques completos al azar, con siete tratamientos y cuatro repeticiones. Los tratamientos correspondieron a los cinco clones CIP y las dos variedades regionales. Los resultados de la investigación permitieron cuantificar el alto potencial productivo de la batata como especie cultivada, encontrándose materiales muy promisorios que pueden ser incorporados a modelos agropecuarios de producción.

  19. Morphological and genetic differentiation and reproductive isolation among closely related taxa in the Ipomoea series Batatas.

    Science.gov (United States)

    Duncan, Tanya M; Rausher, Mark D

    2013-11-01

    Identifying recently diverged taxa can be useful for studying the process of speciation. Ipomoea lacunosa and I. cordatotriloba, along with a putative homoploid hybrid, I. ×leucantha, are closely related taxa, which are promising for investigating the early stages of speciation. The objectives of this investigation were to determine how distinct these purported taxa are morphologically and genetically, and to assess the magnitude of reproductive isolation among the taxa. We measured morphological characteristics and determined genotypes at four microsatellite loci in several populations of each of the taxa in North Carolina and South Carolina to quantify genetic and morphological differentiation. We also included a previously undescribed fourth taxon, which we term 'I. austinii'. Our study revealed that all four taxa had distinct but overlapping geographical ranges, and had significantly distinct morphologies. Patterns of microsatellite variation and the results of crosses indicate that I. ×leucantha and I. austinii are morphologically and genetically distinct taxa. Each exhibits substantial reproductive isolation from the other three taxa. By contrast, microsatellite markers indicate that I. lacunosa and I. cordatotriloba exhibit little differentiation at neutral markers, despite substantial morphological differentiation, and exhibit some reproductive isolation. I. ×leucantha and I. austinii should be considered separate species. Our results provide no evidence that either species originated through homoploid hybrid speciation. I. cordatotriloba and I. lacunosa should be considered incipient species, but may be experiencing considerable reciprocal gene flow.

  20. A new method to culture sweetpotato in space farming

    Science.gov (United States)

    Tsuyuki, I.; Ishii, Y.; Oda, M.; Kitaya, Y.; Mori, G.

    Sweetpotato production in space has many advantages over that of other crops; the plant has a higher growth rate and a higher yield with less fertilizer and less water, and functions as an efficient CO_2/O_2 converter. In a limited space in space farming, however, it is not favorable that sweetpotato shoots develop vigorously while the roots have not enlarged yet, because the sweetpotato organ of interest is not the shoot but the tuberous root. Cuttings of sweetpotato (Ipomoea batatas Lam. "Beniazuma") were used in this study. Each cutting was cut off from the 2nd - 10th nodes from the apices of mother branches and consisted of one expanded leaf, one node and five cm long stem. The cuttings were cultured suboptimally on a mixed soil (peat-moss:vermiculite=1:1 in volume) in a greenhouse under sunlight. Growth characteristics of the cuttings removed axillary buds were compared with cuttings with axillary buds in the first experiment. The cuttings without axillary buds started tuberous root bulking about 30 days after the onset of the experiment. The harvest index (tuberous root dry mass/total dry mass) was 0.5 after 70 days. Whereas, the control plant with an axillary bud developed a lateral shoot and formed no tuberous root during 70 days in the experiment. It was necessary to remove the axillary buds in order to form the tuberous roots in this method. To evaluate the effect of light intensity on tuberous root formation, cuttings without axillary buds were shaded with cheesecloth having 43% of light transmittance in the second experiment. The tuberous root formation was retarded 50 days in shaded cuttings compared with control cuttings. The tuberous roots were quickly formed and the large harvest index was ensured in this method with cuttings without axillary buds. Therefore the method is expected to be advantageous to culture sweetpotato at a high density with rapid turn over in a limited culture space in space farming.

  1. Transcriptome profiling of sweetpotato tuberous roots during low temperature storage.

    Science.gov (United States)

    Ji, Chang Yoon; Chung, Won-Hyong; Kim, Ho Soo; Jung, Won Yong; Kang, Le; Jeong, Jae Cheol; Kwak, Sang-Soo

    2017-03-01

    Sweetpotato [Ipomoea batatas (L.) Lam] is a globally important root crop with high industrial value. However, because sweetpotato tuberous roots undergo chilling injuries that negatively affect their quality at temperatures below 10 °C, postharvest damage during the winter season is a major constraint for industrialization. To understand chilling injury response during postharvest low temperature storage, we used next-generation sequencing technology to comprehensive analyze the transcriptome of tuberous roots stored at optimal (13 °C) or low temperature (4 °C) for 6 weeks. From nine cDNA libraries, we produced 298,765,564 clean reads, which were de novo assembled into 58,392 unigenes with an average length of 1100 bp. A total of 3216 differentially expressed genes (DEGs) were detected and categorized into six clusters, of which clusters 2, 4, and 5 (1464 DEGs) were up-regulated under low temperature. The genes in these three clusters are involved in biosynthesis of unsaturated fatty acids, pathogen defense, and phenylalanine metabolism. By contrast, genes in clusters 1, 3, and 6 (1752 DEGs), which were generally down-regulated at low temperature, encode antioxidant enzymes or are involved in glycerophospholipid, carbohydrate, or energy metabolism. We confirmed the results of the transcriptome analysis by quantitative RT-PCR. Our transcriptome analysis will advance our understanding of the comprehensive mechanisms of chilling injury during low temperature storage and facilitate improvements in postharvest storage of sweetpotato tuberous roots. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  2. Caracterização de variedades de batata doce (Ipomoea batatas L. através de descritores morfológicos e isoenzimáticos Morphological and isoenzymatic characterization of sweet potato (Ipomoea batatas L.

    Directory of Open Access Journals (Sweden)

    Eliane Augustin

    2000-03-01

    Full Text Available Ocorre grande variabilidade no germoplasma de batata-doce disponível no Rio Grande do Sul, mas nem todas populações podem ser caracterizadas através de aspectos morfológicos, havendo necessidade de recorrer a outros métodos, como análise de isoenzimas, para a sua diferenciação. A avaliação de características morfológicas e isoenzimáticas de dez acessos do Banco de Germoplasma de Hortaliças da Embrapa Clima Temperado foi o objetivo deste trabalho. Quatro descritores morfológicos foram utilizados, permitindo distinguir seis variedades. Análises de isoenzimas de peroxidase, aspartato transaminase e esterase foram efetuadas, utilizando-se eletroforese horizontal em gel de poliacrilamida. Foi observada similaridade máxima (1,00 estimada através do coeficiente de Jaccard, com base nesses marcadores, apenas entre as variedades Americana e Morada INTA. A análise de agrupamento permitiu a distinção de dois grupos e quatro subgrupos, utilizando UPGMA (método da média aritmética não ponderada. Os resultados obtidos permitiram a diferenciação dos dez acessos estudados e indicaram que a análise de isoenzimas associada a avaliações morfológicas é de grande valia na caracterização de germoplasma de batata-doce disponível no Rio Grande do Sul.Despite the variability observed in sweet potato germplasm found in the Rio Grande do Sul State, many populations can not be morphologically characterized without the use of additional methods, such as isoenzyme analysis. The aim of this work was to evaluate the morphological and isoenzymatic characteristics of ten accesses of the genetic resources program of Embrapa Clima Temperado. Four morphological descriptors were used, which allowed to distinguish six varieties. Polyacrylamyde electrophoresis was used to analyze peroxidase, aspartate transaminase and esterase isoenzymes. Maximum similarity (1.00, estimated through Jaccard coefficient, was observed only in comparisons between

  3. Enhanced accumulation of carotenoids in sweetpotato plants overexpressing IbOr-Ins gene in purple-fleshed sweetpotato cultivar.

    Science.gov (United States)

    Park, Sung-Chul; Kim, Sun Ha; Park, Seyeon; Lee, Hyeong-Un; Lee, Joon Seol; Park, Woo Sung; Ahn, Mi-Jeong; Kim, Yun-Hee; Jeong, Jae Cheol; Lee, Haeng-Soon; Kwak, Sang-Soo

    2015-01-01

    Sweetpotato [Ipomoea batatas (L.) Lam] is an important root crop that produces low molecular weight antioxidants such as carotenoids and anthocyanin. The sweetpotato orange (IbOr) protein is involved in the accumulation of carotenoids. To increase the levels of carotenoids in the storage roots of sweetpotato, we generated transgenic sweetpotato plants overexpressing IbOr-Ins under the control of the cauliflower mosaic virus (CaMV) 35S promoter in an anthocyanin-rich purple-fleshed cultivar (referred to as IbOr plants). IbOr plants exhibited increased carotenoid levels (up to 7-fold) in their storage roots compared to wild type (WT) plants, as revealed by HPLC analysis. The carotenoid contents of IbOr plants were positively correlated with IbOr transcript levels. The levels of zeaxanthin were ∼ 12 times elevated in IbOr plants, whereas β-carotene increased ∼ 1.75 times higher than those of WT. Quantitative RT-PCR analysis revealed that most carotenoid biosynthetic pathway genes were up-regulated in the IbOr plants, including PDS, ZDS, LCY-β, CHY-β, ZEP and Pftf, whereas LCY-ɛ was down-regulated. Interestingly, CCD1, CCD4 and NCED, which are related to the degradation of carotenoids, were also up-regulated in the IbOr plants. Anthocyanin contents and transcription levels of associated biosynthetic genes seemed to be altered in the IbOr plants. The yields of storage roots and aerial parts of IbOr plants and WT plants were not significantly different under field cultivation. Taken together, these results indicate that overexpression of IbOr-Ins can increase the carotenoid contents of sweetpotato storage roots. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  4. A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers

    Science.gov (United States)

    2010-01-01

    Background Sweetpotato (Ipomoea batatas (L.) Lam.), a hexaploid outcrossing crop, is an important staple and food security crop in developing countries in Africa and Asia. The availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. Previously existing sequence data were restricted to around 22,000 expressed sequence tag (EST) sequences and ~ 1,500 GenBank sequences. We have used 454 pyrosequencing to augment the available gene sequence information to enhance functional genomics and marker design for this plant species. Results Two quarter 454 pyrosequencing runs used two normalized cDNA collections from stems and leaves from drought-stressed sweetpotato clone Tanzania and yielded 524,209 reads, which were assembled together with 22,094 publically available expressed sequence tags into 31,685 sets of overlapping DNA segments and 34,733 unassembled sequences. Blastx comparisons with the UniRef100 database allowed annotation of 23,957 contigs and 15,342 singletons resulting in 24,657 putatively unique genes. Further, 27,119 sequences had no match to protein sequences of UniRef100database. On the basis of this gene index, we have identified 1,661 gene-based microsatellite sequences, of which 223 were selected for testing and 195 were successfully amplified in a test panel of 6 hexaploid (I. batatas) and 2 diploid (I. trifida) accessions. Conclusions The sweetpotato gene index is a useful source for functionally annotated sweetpotato gene sequences that contains three times more gene sequence information for sweetpotato than previous EST assemblies. A searchable version of the gene index, including a blastn function, is available at http://www.cipotato.org/sweetpotato_gene_index. PMID:20977749

  5. Dietary sweet potato (Ipomoea batatas L.) leaf extract attenuates hyperglycaemia by enhancing the secretion of glucagon-like peptide-1 (GLP-1).

    Science.gov (United States)

    Nagamine, Rika; Ueno, Shiori; Tsubata, Masahito; Yamaguchi, Kazuya; Takagaki, Kinya; Hira, Tohru; Hara, Hiroshi; Tsuda, Takanori

    2014-09-01

    'Suioh', a sweet potato (Ipomoea batatas L.) cultivar developed in Japan, has edible leaves and stems. The sweet potato leaves contain polyphenols such as caffeoylquinic acid (CQA) derivatives. It has multiple biological functions and may help to regulate the blood glucose concentration. In this study, we first examined whether sweet potato leaf extract powder (SP) attenuated hyperglycaemia in type 2 diabetic mice. Administration of dietary SP for 5 weeks significantly lowered glycaemia in type 2 diabetic mice. Second, we conducted in vitro experiments, and found that SP and CQA derivatives significantly enhanced glucagon-like peptide-1 (GLP-1) secretion. Third, pre-administration of SP significantly stimulated GLP-1 secretion and was accompanied by enhanced insulin secretion in rats, which resulted in a reduced glycaemic response after glucose injection. These results indicate that oral SP attenuates postprandial hyperglycaemia, possibly through enhancement of GLP-1 secretion.

  6. Construction of a genetic map using EST-SSR markers and QTL analysis of major agronomic characters in hexaploid sweet potato (Ipomoea batatas (L.) Lam).

    Science.gov (United States)

    Kim, Jin-Hee; Chung, Il Kyung; Kim, Kyung-Min

    2017-01-01

    The Sweet potato, Ipomoea batatas (L.) Lam, is difficult to study in genetics and genomics because it is a hexaploid. The sweet potato study not have been performed domestically or internationally. In this study was performed to construct genetic map and quantitative trait loci (QTL) analysis. A total of 245 EST-SSR markers were developed, and the map was constructed by using 210 of those markers. The total map length was 1508.1 cM, and the mean distance between markers was 7.2 cM. Fifteen characteristics were investigated for QTLs analysis. According to those, the Four QTLs were identified, and The LOD score was 3.0. Further studies need to develop molecular markers in terms of EST-SSR markers for doing to be capable of efficient breeding. The genetic map created here using EST-SSR markers will facilitate planned breeding of sweet potato cultivars with various desirable traits.

  7. Ipomoea batatas (Convolvulaceae)-mediated synthesis of silver nanoparticles for controlling mosquito vectors of Aedes albopictus, Anopheles stephensi, and Culex quinquefasciatus (Diptera:Culicidae).

    Science.gov (United States)

    Pavithra Bharathi, V; Ragavendran, C; Murugan, N; Natarajan, D

    2017-12-01

    We proposed an effective and eco-friendly control of dengue, malaria, and filariasis-causing vectors. We tested Ipomoea batatas leaves-mediated silver nanoparticles (AgNPs) against first to fourth instar larvae and adults of Aedes albopictus, Anopheles stephensi, and Culex quinquefasciatus at different concentrations. The synthesized AgNPs showed broad spectrum of larvicidal and adulticidal effects after 48 h of exposure. The characterization of synthesized AgNPs was done using various spectral and microscopy analyses. The maximum efficacy was observed in synthesized AgNPs against the adult of Ae. albopictus with the LC 50 and LC 90 values were 10.069 and 15.657 μg/mL, respectively, followed by others.

  8. Construction of a genetic map using EST-SSR markers and QTL analysis of major agronomic characters in hexaploid sweet potato (Ipomoea batatas (L.) Lam)

    Science.gov (United States)

    Kim, Jin-Hee; Chung, Il Kyung

    2017-01-01

    The Sweet potato, Ipomoea batatas (L.) Lam, is difficult to study in genetics and genomics because it is a hexaploid. The sweet potato study not have been performed domestically or internationally. In this study was performed to construct genetic map and quantitative trait loci (QTL) analysis. A total of 245 EST-SSR markers were developed, and the map was constructed by using 210 of those markers. The total map length was 1508.1 cM, and the mean distance between markers was 7.2 cM. Fifteen characteristics were investigated for QTLs analysis. According to those, the Four QTLs were identified, and The LOD score was 3.0. Further studies need to develop molecular markers in terms of EST-SSR markers for doing to be capable of efficient breeding. The genetic map created here using EST-SSR markers will facilitate planned breeding of sweet potato cultivars with various desirable traits. PMID:29020092

  9. Potencial productivo de esquejes de boniato (Ipomoea batatas (L. Lam. procedentes de una nueva tecnología de producción de semilla agámica.

    Directory of Open Access Journals (Sweden)

    Roberto Díaz Hernández

    2012-01-01

    Full Text Available RESUMEN El trabajo se realizó en el Instituto de Investigaciones en Viandas Tropicales durante el periodo de septiembre del 2008 a abril 2009, con los objetivos de determinar la cantidad de esquejes que proporciona el vivero y la frecuencia de corte, así como comparar el potencial productivo de cada corte. El Experimento consistió en el montaje de cámaras de aproximadamente 1m de ancho y 15m de largo, se utilizaron raíces tuberosas del clon comercial INIVIT B2-2005 como material de plantación. Se comprobó que durante la fase del vivero este proporcionó un total de nueve cortes tanto en la época frío como en primavera con un total de 764 y 1390 esquejes respectivamente. Después de cortados los esquejes fueron llevados al campo donde se realizó un estudio para determinar los rendimientos correspondientes a cada tratamiento (número de cortes. Se utilizó un diseño de bloques al azar con cuatros réplicas. Los resultados mostraron que en pequeñas áreas de viveros se puede obtener un número significativo de esquejes y de muy buena calidad productiva además de que los esquejes procedentes de diferentes momentos de cortes presentan igual comportamiento en cuanto al rendimiento. Productive potential of sweetpotato (Ipomoea batatas (L. Lam cuttings from a new technology for agamic seed production. ABSTRACT The work was conducted at the Research Institute of Tropical Root and Tuber Crops from September 2008 to April 2009 with the objectives of determining the amount of cuts provided by the nursery and cut frequency, as well as, to compare the productive potential of each cut. The experiment consisted of mounting chambers about 1m wide and 15m long. Tuberous roots from the commercial clone INIVIT B2-2005 were used as planting seeds. During the nursery stage, nine cuts were provided in the winter and rainy seasons with a total of 764 and 1390 cuttings respectively. Cuttings were transferred to field conditions to determine corresponding

  10. A novel α/β-hydrolase gene IbMas enhances salt tolerance in transgenic sweetpotato.

    Directory of Open Access Journals (Sweden)

    Degao Liu

    Full Text Available Salt stress is one of the major environmental stresses in agriculture worldwide and affects crop productivity and quality. The development of crops with elevated levels of salt tolerance is therefore highly desirable. In the present study, a novel maspardin gene, named IbMas, was isolated from salt-tolerant sweetpotato (Ipomoea batatas (L. Lam. line ND98. IbMas contains maspardin domain and belongs to α/β-hydrolase superfamily. Expression of IbMas was up-regulated in sweetpotato under salt stress and ABA treatment. The IbMas-overexpressing sweetpotato (cv. Shangshu 19 plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbMas up-regulated the salt stress responsive genes, including pyrroline-5-carboxylate synthase, pyrroline-5-carboxylate reductase, SOD, psbA and phosphoribulokinase genes, under salt stress. These findings suggest that overexpression of IbMas enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and increasing reactive oxygen species scavenging capacity.

  11. Variability of sugars in staple-type sweetpotato (Ipomoea batatas) cultivars: The effects of harvest time and storage

    Science.gov (United States)

    Total soluble sugar content and composition was studied by high performance liquid chromatography in four high dry-matter sweet potato cultivars at 3, 4, and 5 months maturity. Total soluble sugar consisted of sucrose, glucose, and fructose, ranging from 4.10–10.82 g/100 g (dry-weight basis). At har...

  12. Antioxidants and α-glucosidase inhibitors from Ipomoea batatas leaves identified by bioassay-guided approach and structure-activity relationships.

    Science.gov (United States)

    Zhang, Lu; Tu, Zong-Cai; Yuan, Tao; Wang, Hui; Xie, Xing; Fu, Zhi-Feng

    2016-10-01

    Sweet potato (Ipomoea batatas) leaf (SPL) is an underused commercial vegetable with considerable bio-activities. By means of DPPH scavenging ability and α-glucosidase inhibitory oriented isolation, 9 and 7 compounds were isolated and identified, respectively. Among them, trans-N-(p-coumaroyl)tyramine (1), trans-N-feruloyltyramine (2), cis-N-feruloyltyramine (3), 4,5-feruloylcourmaoylquinic acid (8), caffeic acid ethyl ester (10), 7-hydroxy-5-methoxycoumarin (11), 7,3'-dimethylquercetin (13) and indole-3-carboxaldehyde (15), were firstly identified from SPL, and four of them (1, 2, 3 and 10) were firstly identified from genus Ipomoea. Phenethyl cinnamides and 3,4,5-triCQA exhibited the strongest α-glucosidase inhibition, while 3,4,5-triCQA and diCQAs were the dominant antioxidants. Structure-activity relationship revealed that higher caffeoylation of quinic acid and lower methoxylation of flavonols resulted in stronger antioxidant activity, and methylation and cis-configuration structure of phenethyl cinnamides weaken the α-glucosidase inhibition. Aforementioned results could help to explain the antioxidant activity and anti-diabetic activity of SPL, and provide theoretical basis for its further application. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Etude du comportement au champ et des performances des variétés locales de patate douce Ipomoea batatas dans la sous-région de Ht un, à l'Est du Zaïre

    OpenAIRE

    Ngoy Kadiebwe, N.

    1993-01-01

    Study of the behaviour in the field and of local varieties performances of sweet potato Ipomoea batatas in eastern Zaire. The most cultivated twenty local varieties of Sweet Potato in Ituri District have been put in competition al INERA-NIOKA Research Center in order to compare their performances. Five of them have been eliminated during undergoing trials owing to their low productivity. The Kenya variety with yellowish skin and yellow flesh has proved itself to be resistant to viroses attack...

  14. Caracterización postcosecha y composición química de la batata (Ipomoea batatas (L. Lamb. variedad Topera.

    Directory of Open Access Journals (Sweden)

    Auris Damely García-Méndez

    2016-06-01

    Full Text Available El objetivo de este trabajo fue caracterizar la composición química y calidad postcosecha de la batata variedad Topera proveniente de una producción semi-mecanizada con buenas prácticas agrícolas (BPA. El estudio se realizó en el Laboratorio de Procesamiento Primario de Productos Agrícolas y Bioquímica de Alimentos de la Facultad de Agronomía (FAGRO de la Universidad Central de Venezuela (UCV durante el año 2014. La metodología permitió determinar algunas características físicas, texturales y composición química por los métodos de AOAC (Association of Official Analytical Chemists. Se obtuvieron batatas con un menor coeficiente de variabilidad en el peso (CV: 20,12%, forma (CV: 5,63% y tamaño (CV: 12,80%. Esta respuesta permitió establecer tres categorías de calidad con una baja incidencia de defectos (2,15% y daños físico-mecánicos (30,25%, esta última asociada a la alta resistencia del material (6,25+0,05 kgf/mm. Las batatas presentaron contenidos de azúcares reductores de 9,88%, fibra de 6,19% y proteína de 4,13%; estas características nutricionales sugieren su uso como suplemento alimenticio. La producción semi-mecanizada con la implementación de las técnicas de las BPA, permitió obtener batatas con características de importancia para la agroindustria procesadora de alimentos IV Gama y, a nivel del consumidor, con una calidad física homogénea.

  15. Profiling of differentially expressed genes critical to storage root development in hydroponically and in-vitro grown sweetpotato for space farming

    Science.gov (United States)

    Egnin, M.; Gao, H.; He, G.; Woullard, F.; Mortley, D.; Scoffield, J.; Bey, B.; Quain, M.; Prakash, C. S.; Bonsi, C.

    Environment is known to have significant effects on the nutrient content and quality of crop plants especially through its impact on the temporal and spatial expression of genes Little is known about the molecular changes and harvest index in plants in response to microgravity Sweetpotato underline Ipomoea underline batatas L Lam is one of the most important root crops and an excellent NASA crop for space farming to provide essential nutrients to sustain human life on long-term space missions The initiation and development of storage root formation is one of the most critical processes determining yield of sweetpotato The molecular mechanism of storage root initiation and development in sweetpotato is poorly understood To this end knowledge of gravity perception the genetic and molecular nature of the induction process of storage root will tremendously help improve on sweetpotato harvest index for space farming cDNA-AFLP techniques were employed to investigate temporal and spatial expressions to gain molecular insights and identify transcripts differentially expressed during early stages of sweetpotato storage root development Two hydroponically grown cultivars using Nutrient Film Technology NFT and microstorage roots were evaluated TU-82-155 an early maturing 90 DAP with orange flesh and tinge red skin and PI318846-3 a late maturing 135 DAP with white flesh and off-yellow skin were compared for differential genes expression during storage root development at 14 21 28 35 and 45 DAP Total RNA was isolated from

  16. Stable internal reference genes for the normalization of real-time PCR in different sweetpotato cultivars subjected to abiotic stress conditions.

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    Sung-Chul Park

    Full Text Available Reverse transcription quantitative real-time PCR (RT-qPCR has become one of the most widely used methods for gene expression analysis, but its successful application depends on the stability of suitable reference genes used for data normalization. In plant studies, the choice and optimal number of reference genes must be experimentally determined for the specific conditions, plant species, and cultivars. In this study, ten candidate reference genes of sweetpotato (Ipomoea batatas were isolated and the stability of their expression was analyzed using two algorithms, geNorm and NormFinder. The samples consisted of tissues from four sweetpotato cultivars subjected to four different environmental stress treatments, i.e., cold, drought, salt and oxidative stress. The results showed that, for sweetpotato, individual reference genes or combinations thereof should be selected for use in data normalization depending on the experimental conditions and the particular cultivar. In general, the genes ARF, UBI, COX, GAP and RPL were validated as the most suitable reference gene set for every cultivar across total tested samples. Interestingly, the genes ACT and TUB, although widely used, were not the most suitable reference genes in different sweetpotato sample sets. Taken together, these results provide guidelines for reference gene(s selection under different experimental conditions. In addition, they serve as a foundation for the more accurate and widespread use of RT-qPCR in various sweetpotato cultivars.

  17. Ipomoea batatas (L.)

    African Journals Online (AJOL)

    La patate douce est une culture négligée alors que sa contribution dans la sécurité alimentaire est importante au Bénin. Ce travail a permis ... l'alimentation animale (Owori et al., 2007). Dans l'industrie, les tubercules servent à la ... douce et la valorisation des tubercules par la fabrication de farines infantiles ont été ...

  18. Ipomoea batatas L.

    African Journals Online (AJOL)

    UKZN

    2012-10-18

    MS) media supplemented with 36 combinations .... percentage shoot generation was computed as the proportion of explants showing shoots .... Feng C, Yin Z, Ma Y, Zhang Z, Chen L, Wang B, Li B, Huang Y, Wang. Q (2011).

  19. ipomoea batatas (l

    African Journals Online (AJOL)

    preferred customer

    MATERIALS AND METHODS. Field study. Reconnaissance soil survey was conducted in ... caliper and meter-tape, respectively, and plant was harvested at the end of the seventh week. Soil sample was taken ... has a low available phosphorous as well as nitrogen. The percent base saturation was high and calcium took ...

  20. Produção da batata-doce adubada com esterco bovino e biofertilizante Yield of sweet potato (Ipomoea batatas (L. Lam. fertilized with cattle manure and biofertilizer

    Directory of Open Access Journals (Sweden)

    Ademar Pereira de Oliveira

    2007-12-01

    Full Text Available Objetivou-se avaliar a produção da batata-doce (Ipomoea batatas (L. Lam. adubada com esterco bovino e biofertilizante, conduziu-se um experimento no período de Janeiro a Junho de 2004, em NEOSSOLO REGOLÍTICO, psamítico típico, na Universidade Federal da Paraíba, em Areia-PB. O delineamento experimental empregado foi de blocos casualisados, com quatro repetições com os tratamentos distribuídos em arranjo fatorial 6 x 2, sendo seis doses de esterco bovino (0, 10, 20, 30, 40, 50 t ha-1 e ausência e presença de biofertilizante. As parcelas foram constituídas por 80 plantas, espaçadas de 80 x 30 cm. O número de raízes comerciais por planta atingiu valores máximos estimados de 2,0 e 1,7 raízes, respectivamente, nas doses de 26 e 25 t ha-1 de esterco bovino na presença e ausência de biofertilizante. O peso médio de raízes comerciais da batata-doce aumentou com elevação das doses de esterco bovino, atingindo na maior dose 360 e 224 g na presença e ausência de biofertilizante. As doses de 25,6 e 24,4 t ha-1 de esterco bovino foram responsáveis pela máxima produção total de raízes de 21,4 e 21,2 t ha-1, respectivamente, na presença e ausência de biofertilizante. As máximas produções de raízes comerciais, 15,2 e 12,9 t ha-1 foram alcançadas com 25,5 e 21,3 t ha-1 de esterco bovino, na presença e ausência de biofertilizante, respectivamente.Aiming to evaluate the sweet potato (Ipomoea batatas (L. Lam. yield fertilization with bovine manure and biofertilizer, an experiment was carried out from January to July, 2004, on ustipsamment soil, at Universidade Federal da Paraíba, Areia-PB. The Experimental outline used was randomized blocks, with four replications and, treatments distributed in factorial arrangement 6 x 2, being six levels of bovine manure (0, 10, 20, 30, 40, 50 t ha-1 and absence and presence of biofertilizer. Plots were constituted by 80 plants, spaced 80 x 30 cm. Commercial roots number per plant reached

  1. Ocorrência da broca do coleto (Megastes spp. no banco de germoplasma de batata-doce (Ipomoea batatas (L. Lam do SMGP-CECA/UFAL

    Directory of Open Access Journals (Sweden)

    Jair Tenório Cavalcante

    2013-09-01

    Full Text Available O presente trabalho teve como objetivo avaliar a ocorrência da Broca do Coleto (Megastes spp. no banco de germoplasma de batata doce (Ipomea batatas (L.Lam.  do Setor de Melhoramento Genético de Plantas do Centro de Ciências Agrárias da Universidade Federal de Alagoas (SMPG/CECA/UFAL, no município de Rio Largo – AL, no ano de 2011. Utilizou-se o delineamento inteiramente casualizado com 14 tratamentos, em duas épocas de plantio (estação chuvosa e estação seca e com cinco avaliações (aos 30, 45, 60, 75 e 90 dias após o plantio - DAP. Os genótipos avaliados foram: 12 clones obtidos pelo SMGP e duas variedades: Sergipana e Rainha de Penedo. As avaliações foram realizadas após o trigésimo dia do plantio através de escala de notas segundo a metodologia idealizada por Azevedo et. al., (1996. Os resultados permitiram concluir que há um ataque mais intenso da broca durante a época estação seca, a maior ocorrência aos 90 dias após o plantio, certamente não causará redução significativa na produção de raízes tuberosas, pois nesta fase a cultura praticamente já se encontra em condições de colheita. Os clones 2, 4, 6 e 14 e as variedades Sergipana e Rainha de Penedo, são de grande valia para o programa de melhoramento genético da cultura da batata-doce. 

  2. Six Peptide Wound Signals Derived from a Single Precursor Protein in Ipomoea batatas Leaves Activate the Expression of the Defense Gene Sporamin*

    Science.gov (United States)

    Chen, Yu-Chi; Siems, William F.; Pearce, Gregory; Ryan, Clarence A.

    2008-01-01

    A mixture of three homologous bioactive hydroxyproline-rich glycopeptides (HypSys peptides) of 18 amino acids in length, differing only at two residues, was isolated from leaves of Ipomoea batatas, the common sweet potato. One of the peptides represented over 95% of the isolated isopeptides, which, at 2.5 nm concentration, induced the expression of sporamin, a major defense protein of I. batatas. The sequence of the major isoform was used to synthesize a primer that identified a cDNA encoding a precursor protein. The protein contained six proline-rich regions whose sequences suggested that they might be HypSys defense signals. One of the encoded peptides, called IbHypSys IV, was identical to one of two minor components of the isolated isopeptides, but neither the major isopeptide nor the other minor isoform was found within the precursor. The six peptides encoded by the precursor gene were synthesized but with hydroxyproline residues at positions found in the native isoforms and lacking carbohydrate moieties. All of the peptides were biologically active when supplied to leaves of sweet potato plants. The gene is the first ortholog of the preproHypSys gene family to be found outside of the Solanaceae family, and its encoded peptide precursor is the first example in plants of a precursor protein with six potential peptide defense signals, a scenario only found previously in animals. The data indicate that multiple copies of the HypSys peptides in a single precursor may have an important role in amplifying wound signaling in leaves in response to herbivore attacks. PMID:18299332

  3. Características físicas e sensoriais de clones de batata-doce Physical and sensorial characteristics of sweetpotato clones

    Directory of Open Access Journals (Sweden)

    Adriana Dias Cardoso

    2007-12-01

    Full Text Available Com o objetivo de avaliar propriedades físicas e sensoriais de clones de batata-doce em Vitória da Conquista - BA foi realizado este experimento, composto por 16 clones oriundos de Janaúba- G, Viçosa - MG, Bom Jardim de Minas - MG, Gurupi - TO, Santo Antônio da Platina - PR, Holambra II - SP, Vitória da Conquista - BA e Condeúba - BA. Utilizou-se o delineamento em blocos casualizados, com 16 tratamentos e 3 repetições. Avaliaram-se as características sensoriais: aparência, umidade, doçura, coloração da polpa, dificuldade de deglutição das raízes tuberosas e as características físicas: tempo de cozimento e peso específico. Os dados foram submetidos à análise de variância e teste de Scott-Knott a 5% de probabilidade, entretanto, as características sensoriais foram obtidas apenas em valores de porcentagem. O clone 25 apresentou as melhores características sensoriais e o clone 7 apresentou melhor tempo de cozimento.The aim of this experiment was to evaluate the physical and sensorial characteristics of sweetpotato clones in Vitória da Conquista, Bahia State, Brazil. Sixteen clones were analyzed, originating from Janaúba, MG, Viçosa, MG; Bom Jardim de Minas, MG; Gurupi, TO; Santo Antônio da Platina, PR; Holambra II, SP; Vitória da Conquista, BA; and Condeúba, BA. One utilized randomized blocks with 16 treatments and three repetitions. The following characteristics were analyzed: aspect, humidity, sweetness, color, deglutition difficulty, cooking and specific gravity of the storage roots. The data were submitted to variance analysis using a ScottKnott test with 5% probability. Clone 25 presented the best sensorial characteristics, and clone 7 presented the best cooking time.

  4. Biocompatibility of sweetpotato and peanut in a hydroponic system

    Science.gov (United States)

    Mortley, D. G.; Loretan, P. A.; Hill, W. A.; Bonsi, C. K.; Morris, C. E.; Hall, R.; Sullen, D.

    1998-01-01

    'Georgia Red' peanut (Arachis hypogaea L.) and TU-82-155 sweetpotato [Ipomoea batatas (L.) Lam] were grown in monocultured or intercropped recirculating hydroponic systems in a greenhouse using the nutrient film technique (NFT). The objective was to determine whether growth and subsequent yield would be affected by intercropping. Treatments were sweetpotato monoculture (SP), peanut monoculture (PN), and sweetpotato and peanut grown in separate NFT channels but sharing a common nutrient solution (SP-PN). Greenhouse conditions ranged from 24 to 33 degrees C, 60% to 90% relative humidity (RH), and photosynthetic photon flux (PPF) of 200 to 1700 micromoles m-2 s-1. Sweetpotato cuttings (15 cm long) and 14-day-old seedlings of peanuts were planted into growth channels (0.15 x 0.15 x 1.2 m). Plants were spaced 25 cm apart within and 25 cm apart between growing channels. A modified half-Hoagland solution with a 1 N: 2.4 K ratio was used. Solution pH was maintained between 5.5 and 6.0 for treatments involving SP and 6.4 and 6.7 for PN. Electrical conductivity (EC) ranged between 1100 and 1200 microS cm-1. The number of storage roots per sweetpotato plant was similar for both SP and SP-PN. Storage root fresh and dry mass were 29% and 36% greater, respectively, for plants in the SP-PN treatment than for plants in the SP treatment. The percent dry mass of the storage roots, dry mass of fibrous and pencil roots, and the length-to-diameter ratio of storage roots were similar for SP and SP-PN sweetpotato plants. Likewise, foliage fresh and dry mass and harvest index were not significantly influenced by treatment. Total dry mass was 37% greater for PN than for SP-PN peanut plants, and pod dry mass was 82% higher. Mature and total seed dry mass and fibrous root dry mass were significantly greater for PN than for SP-PN plants. Harvest index (HI) was similar for both treatments. Root length tended to be lower for seedlings grown in the nutrient solution from the SP-PN treatment.

  5. Evaluation of structure and bioprotective activity of key high molecular weight acylated anthocyanin compounds isolated from the purple sweet potato (Ipomoea batatas L. cultivar Eshu No.8).

    Science.gov (United States)

    Luo, Chun-Li; Zhou, Qing; Yang, Zi-Wei; Wang, Rui-Dan; Zhang, Jiu-Liang

    2018-02-15

    In order to figure out the key acylated anthocyanin compounds accounting for the bioprotective activity of purple sweet potato (Ipomoea batatas L.), ODS packing column, semi-preparative HPLC method, activity evaluation assays, and ultra-high-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS) assays were employed. Additionally, our study revealed that the structures of two acylated monomeric anthocyanins, cyanidin 3-caffeoyl-feruloyl sophoroside-5-glucoside and peonidin 3-dicaffeoyl sophoroside-5-glucoside were found to have the strongest bioprotective activity, which was identified to be closely related with the ortho-dihydroxybenzene structure, suggesting the more the special structures of catechol moieties, such as caffeoyl and cyanidin, the stronger the bioprotective activity will be. Besides, the aglycon of cyanidin had higher antioxidant capacity than the peonidin, and the acylated residues strengthened the capacity which followed the order of caffeoyl>feruloyl>p-hydroxybenzoyl. These results will lay the groundwork for further researching the structure-activity relationships of acylated monomeric anthocyanins from purple sweet potato. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Plastidial α-glucan phosphorylase 1 complexes with disproportionating enzyme 1 in Ipomoea batatas storage roots for elevating malto-oligosaccharide metabolism.

    Science.gov (United States)

    Lin, Yi-Chen; Chang, Shih-Chung; Juang, Rong-Huay

    2017-01-01

    It has been proposed that malto-oligosaccharides (MOSs) are possibly recycled back into amylopectin biosynthesis via the sequential reactions catalyzed by plastidial α-glucan phosphorylase 1 (Pho1) and disproportionating enzyme 1 (Dpe1). In the present study, the reciprocal co-immunoprecipitation experiments using specific antibodies against Pho1 and Dpe1 demonstrated that these two enzymes can form a complex (the PD complex) in Ipomoea batatas storage roots. The immunohistochemistry analyses also revealed the co-localization of Pho1 and Dpe1 in the amyloplasts, and the protein levels of Pho1 and Dpe1 increased gradually throughout sweet potato storage root development. A high molecular weight PD complex was co-purified from sweet potato storage root lysates by size exclusion chromatography. Enzyme kinetic analyses showed that the PD complex can catalyze maltotriose and maltotetraose to generate glucose-1-phosphate in the presence of inorganic phosphate, and it also performs greater Dpe1 activity toward MOSs than does free form Dpe1. These data suggest that Pho1 and Dpe1 may form a metabolon complex, which provides elevated metabolic fluxes for MOS metabolism via a direct transfer of sugar intermediates, resulting in recycling of glucosyl units back into amylopectin biosynthesis more efficiently.

  7. The Administration of Orange Sweet Potato (Ipomoea Batatas L. Extract Increases Sperm Consentration, Mortility and Viability in Male Mice Exposed to Cigarette Smoke

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    Lintang Laila Rizqy

    2017-04-01

    Full Text Available Pendahuluan: Ipomoea batatas L (IPL mengandung antioksidan betakaroten, vitamin C, dan vitamin E. Rokok mengandung ROS yang terbukti menurunkan kualitas spermatozoa. Tujuan: untuk membuktikan bahwa pemberian IPL dapat memperbaiki jumlah, motilitas, dan viabilitas spermatozoa yang dipapar asap rokok. Metode: Penelitian eksperimental dengan post test only control group design, sebanyak 30 ekor mencit jantan, dibagi menjadi 6 kelompok. Kelompok normal (Nor-G, tidak ada interfensi; Kelompok kontrol negative (Neg-G, hanya dipapar asap rokok; Kelompok kontrol positif (Pos-G, hanya mendapat ekstrak IPL 16 mg/ml; Kelompok IPL-15, IPL-16, dan IPL-17, masing-masing dipapar asap rokok dan mendapat ekstrak IPL 15 mg/ml, 16 mg/ml dan 17 mg/ml.  Hasil: hasil uji Post Hoc LSD menunjukkan bahwa jumlah sperma pada IPL-15, IPL-16, dan IPL-17, lebih tinggi bermakna dibanding Neg-G, p 0.05. Kesimpulan:  pemberian ekstrak ubi jalar jingga dapat meningkatkan jumlah, motilitas, dan viabilitas spermatozoa mencit jantan yang dipapar asap rokok.

  8. Efek Ekstrak Bawang Dayak (Eleutherine palmifolia (L.Merr dan Ubi Ungu (Ipomoea batatas L terhadap Penurunan Kadar Kolesterol dan Trigliserida Darah pada Tikus Jantan

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    Anjar Mahardian Kusuma

    2017-02-01

    Full Text Available Bawang Dayak (Eleutherine palmifolia (L.Merr and Ubi Ungu (Ipomoea batatas L. are potential medicinal plant in lowering cholesterol. This study aims to measure the effect of bawang dayak extract and ubi unggu extract on lowering cholesterol and triglyceride blood levels in male rats fed with 10 ml/Kg BW quail egg yolk. The cholesterol and triglyceride blood levels of rats were measured with 50, 100 and 200 mg/Kg BW dose variations for each extract for 14 days. Cholesterol and triglyceride levels were measured at day 15th by ‘Multicare’. As controls were rats fed with 1,26 mg/Kg BW simvastatin, 1 % (b/v Na-CMC, and normal diet. The data was analyzed by Anova statistical tests and LSD (α = 0,05. The result shows that both extracts can reduce cholesterol and triglyceride blood levels with 200mg/Kg BW dose. Bawang dayak extract can reduce cholesterol and triglyceride blood levels to 80.8 ± 9.2 mg/dL and  95 ± 7.9 mg/dL, respectively. Ubi ungu extract can reduce cholesterol and triglyceride blood levels to 72 ± 8.2 mg/dL and 86.4 ± 4.3 mg/dL, respectively.

  9. Plastidial α-glucan phosphorylase 1 complexes with disproportionating enzyme 1 in Ipomoea batatas storage roots for elevating malto-oligosaccharide metabolism

    Science.gov (United States)

    Lin, Yi-Chen; Chang, Shih-Chung; Juang, Rong-Huay

    2017-01-01

    It has been proposed that malto-oligosaccharides (MOSs) are possibly recycled back into amylopectin biosynthesis via the sequential reactions catalyzed by plastidial α-glucan phosphorylase 1 (Pho1) and disproportionating enzyme 1 (Dpe1). In the present study, the reciprocal co-immunoprecipitation experiments using specific antibodies against Pho1 and Dpe1 demonstrated that these two enzymes can form a complex (the PD complex) in Ipomoea batatas storage roots. The immunohistochemistry analyses also revealed the co-localization of Pho1 and Dpe1 in the amyloplasts, and the protein levels of Pho1 and Dpe1 increased gradually throughout sweet potato storage root development. A high molecular weight PD complex was co-purified from sweet potato storage root lysates by size exclusion chromatography. Enzyme kinetic analyses showed that the PD complex can catalyze maltotriose and maltotetraose to generate glucose-1-phosphate in the presence of inorganic phosphate, and it also performs greater Dpe1 activity toward MOSs than does free form Dpe1. These data suggest that Pho1 and Dpe1 may form a metabolon complex, which provides elevated metabolic fluxes for MOS metabolism via a direct transfer of sugar intermediates, resulting in recycling of glucosyl units back into amylopectin biosynthesis more efficiently. PMID:28472155

  10. Bioethanol production from sweet potato (Ipomoea batatas L. flour using co-culture of Trichoderma sp. and Saccharomyces cerevisiae in solid-state fermentation

    Directory of Open Access Journals (Sweden)

    Manas Ranjan Swain

    2013-04-01

    Full Text Available The aim of this work was to study the optimiation of co-culturing of Trichoderma sp. and Saccharomyces cerevisiae (1:4 ratio on sweet potato (Ipomoea batatas L. flour (SPF for the production of bio-ethanol in solid-state fermentation (SSF. Maximum ethanol (172 g/kg substrate was produced in a medium containing 80% moisture, ammonium sulphate 0.2%, pH 5.0, inoculuted with 10% inoculum size and fermented at 30ºC for 72h. .Concomitant with highest ethanol concentration, maximum ethanol productivity (2.8 g/kg substrate/h, microbial biomass (23×10(8 CFU/ g substrate, ethanol yield (47 g/100g sugar consumed and fermentation efficiency (72% were also obtained under these conditions. Cell interaction was observed familiar between the viable cells of Trichoderma sp. and S. cerevisiae when co-cultured. Ethanol production ability by the co-culture was 65 % higher than the single culture of S. cerevisiae from un-saccharified SPF.

  11. Phylogenetic Placement and Morphological Characterization of Sclerotium rolfsii (Teleomorph: Athelia rolfsii) Associated with Blight Disease of Ipomoea batatas in Korea.

    Science.gov (United States)

    Paul, Narayan Chandra; Hwang, Eom-Ji; Nam, Sang-Sik; Lee, Hyeong-Un; Lee, Joon-Seol; Yu, Gyeong-Dan; Kang, Yong-Gu; Lee, Kyeong-Bo; Go, San; Yang, Jung-Wook

    2017-09-01

    In this study, we aimed to characterize fungal samples from necrotic lesions on collar regions observed in different sweetpotato growing regions during 2015 and 2016 in Korea. Sclerotia appeared on the root zone soil surface, and white dense mycelia were observed. At the later stages of infection, mother roots quickly rotted, and large areas of the plants were destroyed. The disease occurrence was monitored at 45 and 84 farms, and 11.8% and 6.8% of the land areas were found to be infected in 2015 and 2016, respectively. Fungi were isolated from disease samples, and 36 strains were preserved. Based on the cultural and morphological characteristics of colonies, the isolates resembled the reference strain of Sclerotium rolfsii . Representative strains were identified as S. rolfsii (teleomorph: Athelia rolfsii ) based on phylogenetic analysis of the internal transcribed spacer and large subunit genes along with morphological observations. To test the pathogenicity, sweetpotato storage roots were inoculated with different S. rolfsii strains. 'Yulmi' variety displayed the highest disease incidence, whereas 'Pungwonmi' resulted in the least. These findings suggested that morphological characteristics and molecular phylogenetic analysis were useful for identification of S. rolfsii .

  12. Development and Identification of SSR Markers Associated with Starch Properties and β-Carotene Content in the Storage Root of Sweet Potato (Ipomoea batatas L.)

    Science.gov (United States)

    Zhang, Kai; Wu, Zhengdan; Tang, Daobin; Lv, Changwen; Luo, Kai; Zhao, Yong; Liu, Xun; Huang, Yuanxin; Wang, Jichun

    2016-01-01

    Sweet potato (Ipomoea batatas L.) is a nutritious food crop and, based on the high starch content of its storage root, a potential bioethanol feedstock. Enhancing the nutritional value and starch quantity of storage roots are important goals of sweet potato breeding programs aimed at developing improved varieties for direct consumption, processing, and industrial uses. However, developing improved lines of sweet potato is challenging due to the genetic complexity of this plant and the lack of genome information. Short sequence repeat (SSR) markers are powerful molecular tools for tracking important loci in crops and for molecular-based breeding strategies; however, few SSR markers and marker-trait associations have hitherto been identified in sweet potato. In this study, we identified 1824 SSRs by using a de novo assembly of publicly available ESTs and mRNAs in sweet potato, and designed 1476 primer pairs based on SSR-containing sequences. We mapped 214 pairs of primers in a natural population comprised of 239 germplasms, and identified 1278 alleles with an average of 5.972 alleles per locus and a major allele frequency of 0.7702. Population structure analysis revealed two subpopulations in this panel of germplasms, and phenotypic characterization demonstrated that this panel is suitable for association mapping of starch-related traits. We identified 32, 16, and 17 SSR markers associated with starch content, β-carotene content, and starch composition in the storage root, respectively, using association analysis and further evaluation of a subset of sweet potato genotypes with various characteristics. The SSR markers identified here can be used to select varieties with desired traits and to investigate the genetic mechanism underlying starch and carotenoid formation in the starchy roots of sweet potato. PMID:26973669

  13. In silico identification and characterization of conserved miRNAs and their target genes in sweet potato (Ipomoea batatas L.) expressed sequence tags (ESTs).

    Science.gov (United States)

    Dehury, Budheswar; Panda, Debashis; Sahu, Jagajjit; Sahu, Mousumi; Sarma, Kishore; Barooah, Madhumita; Sen, Priyabrata; Modi, Mahendra

    2013-01-01

    The endogenous small non-coding micro RNAs (miRNAs), which are typically ~21-24 nt nucleotides, play a crucial role in regulating the intrinsic normal growth of cells and development of the plants as well as in maintaining the integrity of genomes. These small non-coding RNAs function as the universal specificity factors in post-transcriptional gene silencing. Discovering miRNAs, identifying their targets, and further inferring miRNA functions is a routine process to understand normal biological processes of miRNAs and their roles in the development of plants. Comparative genomics based approach using expressed sequence tags (EST) and genome survey sequences (GSS) offer a cost-effective platform for identification and characterization of miRNAs and their target genes in plants. Despite the fact that sweet potato (Ipomoea batatas L.) is an important staple food source for poor small farmers throughout the world, the role of miRNA in various developmental processes remains largely unknown. In this paper, we report the computational identification of miRNAs and their target genes in sweet potato from their ESTs. Using comparative genomics-based approach, 8 potential miRNA candidates belonging to miR168, miR2911, and miR156 families were identified from 23 406 ESTs in sweet potato. A total of 42 target genes were predicted and their probable functions were illustrated. Most of the newly identified miRNAs target transcription factors as well as genes involved in plant growth and development, signal transduction, metabolism, defense, and stress response. The identification of miRNAs and their targets is expected to accelerate the pace of miRNA discovery, leading to an improved understanding of the role of miRNA in development and physiology of sweet potato, as well as stress response.

  14. Development and Identification of SSR Markers Associated with Starch Properties and β-Carotene Content in the Storage Root of Sweet Potato (Ipomoea batatas L.).

    Science.gov (United States)

    Zhang, Kai; Wu, Zhengdan; Tang, Daobin; Lv, Changwen; Luo, Kai; Zhao, Yong; Liu, Xun; Huang, Yuanxin; Wang, Jichun

    2016-01-01

    Sweet potato (Ipomoea batatas L.) is a nutritious food crop and, based on the high starch content of its storage root, a potential bioethanol feedstock. Enhancing the nutritional value and starch quantity of storage roots are important goals of sweet potato breeding programs aimed at developing improved varieties for direct consumption, processing, and industrial uses. However, developing improved lines of sweet potato is challenging due to the genetic complexity of this plant and the lack of genome information. Short sequence repeat (SSR) markers are powerful molecular tools for tracking important loci in crops and for molecular-based breeding strategies; however, few SSR markers and marker-trait associations have hitherto been identified in sweet potato. In this study, we identified 1824 SSRs by using a de novo assembly of publicly available ESTs and mRNAs in sweet potato, and designed 1476 primer pairs based on SSR-containing sequences. We mapped 214 pairs of primers in a natural population comprised of 239 germplasms, and identified 1278 alleles with an average of 5.972 alleles per locus and a major allele frequency of 0.7702. Population structure analysis revealed two subpopulations in this panel of germplasms, and phenotypic characterization demonstrated that this panel is suitable for association mapping of starch-related traits. We identified 32, 16, and 17 SSR markers associated with starch content, β-carotene content, and starch composition in the storage root, respectively, using association analysis and further evaluation of a subset of sweet potato genotypes with various characteristics. The SSR markers identified here can be used to select varieties with desired traits and to investigate the genetic mechanism underlying starch and carotenoid formation in the starchy roots of sweet potato.

  15. Bocadito con alto contenido proteico: un extruido a partir de quinua (Chenopodium quinoa Willd., tarwi (Lupinus mutabilis Sweet y camote (Ipomoea batatas L.

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    Katherine Pérez Ramos

    2017-01-01

    Full Text Available La presente investigación tuvo como objetivo la elaboración de un bocadito extruido de elevado tenor proteico, a partir de quinua ( Chenopodium quinoa Willd., tarwi ( Lupinus mutabilis Sweet y fécula de camote ( Ipomoea batatas L.. Para su formulación se aplicó el método de diseño de mezclas usando la herramienta computacional - estadíst ica Design Expert® versión 7.0, siendo las variables independientes las harinas de quinua, tarwi y fécula de camote; y las variables dependientes el contenido de proteína, índice de expansión, densidad aparente y dureza de los extruidos. La formulación ópt ima se determinó aplicando la prueba de deseabilidad basada en los modelos de regresión ajustado, dicha formulación se obtuvo maximizando el contenido de proteína e índice de expansión; y minimizando la dureza y densidad aparente de los extruidos. La formu lación óptima tuvo un porcentaje de quinua, tarwi y fécula de camote de 57%, 26% y 17 % respectivamente. El análisis fisicoquímico de la fórmula óptima dio como resultado 20,16% de proteína, 2,19 de índice de expansión, 0,220 g/cm 3 densidad aparente y 9,31 N de dureza. La calidad proteica de la formulación óptima fue determinada mediante los ensayos de digestibilidad verdadera y valor biológico verdadero en ratas, obteniéndose 83,5% y 62,9% respectivamente.

  16. In silico identification and characterization of conserved miRNAs and their target genes in sweet potato (Ipomoea batatas L.) Expressed Sequence Tags (ESTs)

    Science.gov (United States)

    Dehury, Budheswar; Panda, Debashis; Sahu, Jagajjit; Sahu, Mousumi; Sarma, Kishore; Barooah, Madhumita; Sen, Priyabrata; Modi, Mahendra Kumar

    2013-01-01

    The endogenous small non-coding micro RNAs (miRNAs), which are typically ~21–24 nt nucleotides, play a crucial role in regulating the intrinsic normal growth of cells and development of the plants as well as in maintaining the integrity of genomes. These small non-coding RNAs function as the universal specificity factors in post-transcriptional gene silencing. Discovering miRNAs, identifying their targets, and further inferring miRNA functions is a routine process to understand normal biological processes of miRNAs and their roles in the development of plants. Comparative genomics based approach using expressed sequence tags (EST) and genome survey sequences (GSS) offer a cost-effective platform for identification and characterization of miRNAs and their target genes in plants. Despite the fact that sweet potato (Ipomoea batatas L.) is an important staple food source for poor small farmers throughout the world, the role of miRNA in various developmental processes remains largely unknown. In this paper, we report the computational identification of miRNAs and their target genes in sweet potato from their ESTs. Using comparative genomics-based approach, 8 potential miRNA candidates belonging to miR168, miR2911, and miR156 families were identified from 23 406 ESTs in sweet potato. A total of 42 target genes were predicted and their probable functions were illustrated. Most of the newly identified miRNAs target transcription factors as well as genes involved in plant growth and development, signal transduction, metabolism, defense, and stress response. The identification of miRNAs and their targets is expected to accelerate the pace of miRNA discovery, leading to an improved understanding of the role of miRNA in development and physiology of sweet potato, as well as stress response. PMID:24067297

  17. The effect of partial substitution of roasted soybean seed with graded levels of sweet potato (Ipomoea batatas) leaf meal on growth performances and carcass characteristics of broiler chickens.

    Science.gov (United States)

    Melesse, Aberra; Alemu, Temesgen; Banerjee, Sandip; Berihun, Kefyalew

    2016-06-30

    This study was designed to assess the effects of partial substitution of roasted soybean seed with sweet potato (Ipomoea batatas) leaf meal (SPLM) on growth performances and carcass components of broiler chickens. The experiment was a completely randomized design consisting of five dietary treatments replicated four times with ten chicks each. The control diet (treatment 1, T1) contained roasted soybean seed as the major protein source without SPLM and treatment diets containing SPLM at the levels of 30 g/kg (treatment 2, T2), 60 g/kg (treatment 3, T3), 90 g/kg (treatment 4, T4) and 120 g/kg (treatment 5, T5) by partially substituting the roasted soybean seed in the control diet. The results indicated that the body weight gain in chickens reared in T1 was (p<0.01) higher than those fed on T3, T4 and T5 diets. The individual feed intake in chickens reared in T1 was (p<0.001) higher than those fed on other treatment diets. The feed conversion ratio (kg feed/kg gain) of chickens fed on T1 was (p<0.05) lower than those reared in T4 and T5 diets. Chickens fed with T2 diet had numerically higher values of slaughter, dressed carcass and breast than those reared in T4 and T5 diets. It can be concluded that the partial substitution of roasted soybean seed with sweet potato leaf might be economically feasible feeding strategy of broiler chickens in smallholder poultry farm settings.

  18. Efforts to Increase Yield of Sweet Potato (Ipomoea batatas L. through Planting Medium On Roof Garden by Shallow Bed System and Plant Container Bag System

    Directory of Open Access Journals (Sweden)

    Sita Wati

    2017-11-01

    Full Text Available Roof garden is an alternative to overcome food security through breeding crops and horticultures of fruits and vegetables. The research was conducted in Malang by applying two planting systems, such as shallow bed (direct cropping and plant container bag by the same planting medium treatments. Research, which applied shallow bed and container bag, was conducted using Randomized Block Design (RBD by 6 treatments of planting medium and 4 replications, such as : Soil (100% (M0, Soil + Charcoal (1:1 (M1, Soil + Cocopeat (1:1 (M2, Soil + Compost (1:1 (M3, Soil + Manure (1:1 (M4, and Soil + Moss (1:1 (M5. Data analysis of the research used analysis of variance, and if any significant influence was found, it would be followed by the Least Significant Difference (LSD at level 5%. And then, it would be followed by T-test as comparison between shallow bed system and container bag system. Parameters that being observed in the research included length of crop, leaf area, numbers of tuber, fresh weight of tuber, dry weight of tuber, and total dry weight of plants. Results of the research showed that the application of medium types of soil and manure (1:1 by shallow bed system on Roof Garden concept may increase numbers of tuber, fresh weight of tuber, dry weight of tuber, and dry weight of Sweet Potato (Ipomoea batatas L. in comparison with the application of soil medium and manure (1:1 by plant container bag system.

  19. Antioxidant activity, phenolic and flavonoids total of ethanolic extract of Ipomoea batata L. leaves (white, yellow, orange, and purple)

    Science.gov (United States)

    Dewijanti, Indah Dwiatmi; Banjarnahor, Sofna D.; Triyuliani, Maryani, Faiza; Meilawati, Lia

    2017-11-01

    Antioxidant activity, phenolic and total flavonoids from sweet potato ethanol extract (Ipomea batatas L.) of different varieties (white, yellow, orange and purple) were studied. Sweet potatoes were collected from Research Centre for Chemistry. Sweet potato leaves have been used for numerous oxidative-associated diseases such as cancer, allergy, aging, HIV and cardiovascular. 1,1-diphenyl-2-picryl hydrazyl (DPPH) method was used to investigate antioxidant activity in leaves, in which the yellow and purple varieties showed the highest and the lowest scavenging activities of 47.65 µg/ ml (IC50) and 87.402 µg/ ml (IC50), respectively. In this study, the yellow leaves showed the highest concentrations of total phenolic and flavonoids contents at 11.293 µg/g and 44.963 µg/g, respectively. Therefore, sweet potato leaves can be used as a prospective natural antioxidant.

  20. Mecanismos de ação de himenópteros parasitóides sobre Megastes spp. (Lepidoptera: Pyralidae em agroecossistema de batata-doce (Ipomoea batatas L. Attack of parasitoids himenoptera on Megastes spp. (Lepidoptera: Pyralidae in sweet potato agroecossystem

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    Paulo Alves Wanderley

    2004-08-01

    Full Text Available Estudaram-se a presença e a ação de himenópteros parasitóides em agroecossistema de batata-doce (Ipomoea batatas L. no município de Pedra de Fogo - PB, sobre o complexo Megastes spp, com o objetivo de identificar as espécies de parasitóides existentes, bem como descrever os principais eventos na ação de parasitismo sobre Megastes grandalis Guenee e Megastes pusialis Snellen. No levantamento direto, em 14% das plantas, existiam fêmeas de microhimenópteros ectoparasitóides da família braconidae atacando lagartas de Megastes spp. No levantamento indireto, constatou-se um ataque de parasitóide em 22% das lagartas, emergindo adultos de sete espécies das famílias braconidae, Chalcididae e Ichneumonidae, com predominância de braconídeos ectoparasitóides. Concluiu-se que a presença de parasitóides no agroecossistema da batata-doce é bastante diversificada e proporcionam uma boa contribuição na mortalidade natural da praga.The presence and action of Hymenoptera parasitoids in agroecossystems of sweet potato (Ipomoea batatas L. in Pedra de Fogo county - PB, Brazil, on Megastes spp. complex were studied, with the objective of identifying parasitoids species and to describe the main aspects on parasitism action on Megastes grandalis Guenee and Megastes pusialis Snellen. On direct evaluation 14% of plants had females of ectopasitoids belonging to the braconidae family. The indirect evaluation showed the occurrence of 22% of worms attacked by parasitoids. The emergence of parasitoids adults of seven species belonging to the braconidae, Chalcididae and Ichneumonidae families has been observed. It was concluded that the presence of parasitoids in sweet potato agroecossystem is diversified and important to suppress the insect pest.

  1. Estimation of the population density of the sweetpotato weevils on the Mariana Islands

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    Gadi V.P. Reddy

    2012-05-01

    Full Text Available The sweetpotato Ipomoea batatas L. (Convolvulaceae has been one of the most important foods for Pacific islanders for centuries. However, the yield levels have been declining in the recent past due to the presence of sweetpotato weevils Cylas formicarius (Fabricius (Coleoptera, Brentidae, Euscepes postfasciatus (Fairmaire and Daealus tuberosus (Zimmer man (Coleoptera, Curculionidae. Therefore, urgent management or eradication methods are sought in the Mariana Islands (Guam, Rota, Saipan, and Tinian. However, the management or eradication of these weevil pests requires accurate assessments of the target pest density. Currently, no advice is provided to growers on the best method for sampling sweetpotato for weevil pests, although pheromone-based traps or chemicals are being used. This study defines the results of field counts designed to adjust relative sampling techniques for three sweetpotato weevil pests by inspecting plants visually and at random in the field with an absolute measure of population density. Significant relationships were detected between the relative four sampling sites between the three weevil pests. In the dry and wet season, 90% and 35.5%, respectively, of population density of C. formicarius was noticed in Rota. This density of the population levels of this species is significantly lower in Saipan, Guam and Tinian. No incidence of E. postfasciatus and D. tuberosus was observed on Guam. However, E. postfasciatus is identified as the second most destructive pest in Rota, Tinian and Saipan in both the dry and wet seasons. Likewise, D. tuberosus is the third major pest as the recorded population density ranged from 12.5% to 2.5%. Also, it is evident from the sampling study that the population densities of all three weevils are significantly higher in the dry season than the wet season.

  2. Overexpression of Arabidopsis P3B increases heat and low temperature stress tolerance in transgenic sweetpotato.

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    Ji, Chang Yoon; Jin, Rong; Xu, Zhen; Kim, Ho Soo; Lee, Chan-Ju; Kang, Le; Kim, So-Eun; Lee, Hyeong-Un; Lee, Joon Seol; Kang, Chang Ho; Chi, Yong Hun; Lee, Sang Yeol; Xie, Yiping; Li, Hongmin; Ma, Daifu; Kwak, Sang-Soo

    2017-08-14

    Sweetpotato (Ipomoea batatas [L.] Lam) is suitable for growth on marginal lands due to its abiotic stress tolerance. However, severe environmental conditions including low temperature pose a serious threat to the productivity and expanded cultivation of this crop. In this study, we aimed to develop sweetpotato plants with enhanced tolerance to temperature stress. P3 proteins are plant-specific ribosomal P-proteins that act as both protein and RNA chaperones to increase heat and cold stress tolerance in Arabidopsis. Here, we generated transgenic sweetpotato plants expressing the Arabidopsis ribosomal P3 (AtP3B) gene under the control of the CaMV 35S promoter (referred to as OP plants). Three OP lines (OP1, OP30, and OP32) were selected based on AtP3B transcript levels. The OP plants displayed greater heat tolerance and higher photosynthesis efficiency than wild type (WT) plants. The OP plants also exhibited enhanced low temperature tolerance, with higher photosynthesis efficiency and less membrane permeability than WT plants. In addition, OP plants had lower levels of hydrogen peroxide and higher activities of antioxidant enzymes such as peroxidase and catalase than WT plants under low temperature stress. The yields of tuberous roots and aerial parts of plants did not significantly differ between OP and WT plants under field cultivation. However, the tuberous roots of OP transgenic sweetpotato showed improved storage ability under low temperature conditions. The OP plants developed in this study exhibited increased tolerance to temperature stress and enhanced storage ability under low temperature compared to WT plants, suggesting that they could be used to enhance sustainable agriculture on marginal lands.

  3. Agronomic evaluation of sweetpotato varieties | Shigwedha | African ...

    African Journals Online (AJOL)

    The Namibia Root Crop Research Project has conducted sweetpotato (Ipomea batatas) variety evaluation for acceptable agronomic trials. Varieties Blesbok, Yan Shu 1, Xushu 18 and Ribbok were recommended for release in the northern Namibia. Varieties Jewel, Excel and TIS3290 performed above average under ...

  4. Feed and fuel: the dual-purpose advantage of an industrial sweetpotato.

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    Mussoline, Wendy A; Wilkie, Ann C

    2017-03-01

    Sustainable agricultural systems must support nutritional requirements, meet the energy demands of a growing population, preserve environmental resources and mitigate climate change. The sweetpotato (Ipomoea batatas L.) is a high-yielding crop that requires minimal fertilization and irrigation, and the CX-1 industrial cultivar offers superior potential for feed and fuel. CX-1 had the highest agronomic fresh vine yield (51.5 t ha -1 ), averaged over two cropping seasons, compared with Hernandez (33.7) and Beauregard (21.8) varieties. CX-1 vines were more nutritional than the table varieties, specifically in regard to relative feed value (205), water-soluble carbohydrates (171 g kg -1 dry matter (DM)), total digestible nutrients (643 g kg -1 DM), metabolizable energy (10.2 MJ kg -1 DM) and organic matter digestibility. Their lower fiber and lignin concentrations contributed to their freshness and digestibility throughout maturity. Significantly higher iron concentrations make the CX-1 vines a valuable, low-fat iron supplement for animal feed. The CX-1 roots also showed the highest bioethanol potential (82.3 g ethanol kg -1 fresh root) compared to Hernandez (64.5) and Beauregard (48.1). The CX-1 industrial sweetpotato is an ideal dual-purpose crop for tropical/subtropical climates that can be utilized as a non-grain-based feedstock for bioethanol production while contributing a valuable, high-yielding nutritional supplement for animal feed. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  5. Etude du comportement au champ et des performances des variétés locales de patate douce Ipomoea batatas dans la sous-région de Ht un, à l'Est du Zaïre

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    Ngoy Kadiebwe, N.

    1993-01-01

    Full Text Available Study of the behaviour in the field and of local varieties performances of sweet potato Ipomoea batatas in eastern Zaire. The most cultivated twenty local varieties of Sweet Potato in Ituri District have been put in competition al INERA-NIOKA Research Center in order to compare their performances. Five of them have been eliminated during undergoing trials owing to their low productivity. The Kenya variety with yellowish skin and yellow flesh has proved itself to be resistant to viroses attacks with a produre and a very high number of tubers. Again, given its very short vegetative cycle (about four months this variety can be used as a witness in further trials with exotic materials.

  6. A ζ-carotene desaturase gene, IbZDS, increases β-carotene and lutein contents and enhances salt tolerance in transgenic sweetpotato.

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    Li, Ruijie; Kang, Chen; Song, Xuejin; Yu, Ling; Liu, Degao; He, Shaozhen; Zhai, Hong; Liu, Qingchang

    2017-09-01

    ζ-Carotene desaturase (ZDS) is one of the key enzymes in carotenoid biosynthesis pathway. However, the ZDS gene has not been applied to carotenoid improvement of plants. Its roles in tolerance to abiotic stresses have not been reported. In this study, the IbZDS gene was isolated from storage roots of sweetpotato (Ipomoea batatas (L.) Lam.) cv. Nongdafu 14. Its overexpression significantly increased β-carotene and lutein contents and enhanced salt tolerance in transgenic sweetpotato (cv. Kokei No. 14) plants. Significant up-regulation of lycopene β-cyclase (β-LCY) and β-carotene hydroxylase (β-CHY) genes and significant down-regulation of lycopene ε-cyclase (ε-LCY) and ε-carotene hydroxylase (ε-CHY) genes were found in the transgenic plants. Abscisic acid (ABA) and proline contents and superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities were significantly increased, whereas malonaldehyde (MDA) content was significantly decreased in the transgenic plants under salt stress. The salt stress-responsive genes encoding pyrroline-5-carboxylate reductase (P5CR), SOD, CAT, ascorbate peroxidase (APX) and POD were found to be significantly up-regulated in the transgenic plants under salt stress. This study indicates that the IbZDS gene has the potential to be applied for improving β-carotene and lutein contents and salt tolerance in sweetpotato and other plants. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  7. Response of Photosynthesis and Yield of Sweetpotato and Peanut to Super-optimal CO2 levels

    Science.gov (United States)

    Bonsi, C.; Bullard, J.; Hileman, D.; Mortley, D.; Hill, J.; Hill, W.; Morrris, C.

    The fate of persons involved in long-term space travel and habitation will depend greatly on the ability to provide food and a livable environment for them In the National Aeronautics and Space Administration NASA Advanced Life Support ALS program photosynthesis of higher plants will be utilized to provide food and oxygen while removing carbon dioxide produced by humans and other heterotrophs as well as transpiring water that can be recycled for drinking This plant-mediated process is collectively referred to as Bioregenerative Life Support Carbon dioxide concentrations on board a space shuttle cabin atmosphere range between 4000 and 6000 mu mol mol -1 CO 2 but with large crews may exceed 10 000- mu mol mol -1 CO 2 Thus it is critical to evaluate the responses of candidate crops to super optimal levels of CO 2 Soybean and potato have been exposed to CO 2 concentrations up to 5000 and 10 000- mu mol mol -1 Very little research has been published about the effects of super-optimal CO 2 levels on sweetpotato and peanut growth and physiology thus indicating a need for extensive research on these plants The aim of this study was to evaluate the effects of super-optimal CO 2 enrichment on growth of TU-82-155 sweetpotato and Georgia Red peanut in a Microporous Tube Membrane MPT using Turface Media and Nutrient Film Technique NFT nutrient delivery systems Sweetpotato Ipomoea batatas L Lam and peanut Arachis hypogaea L were exposed to three CO 2 levels of 400

  8. Genetic variability and evolutionary implications of RNA silencing suppressor genes in RNA1 of sweet potato chlorotic stunt virus isolates infecting sweetpotato and related wild species.

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    Arthur K Tugume

    Full Text Available BACKGROUND: The bipartite single-stranded RNA genome of Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus; Closteroviridae encodes a Class 1 RNase III (RNase3, a putative hydrophobic protein (p7 and a 22-kDa protein (p22 from genes located in RNA1. RNase3 and p22 suppress RNA silencing, the basal antiviral defence mechanism in plants. RNase3 is sufficient to render sweetpotato (Ipomoea batatas virus-susceptible and predisposes it to development of severe diseases following infection with unrelated virus. The incidence, strains and gene content of SPCSV infecting wild plant species have not been studied. METHODOLOGY/PRINCIPAL FINDINGS: Thirty SPCSV isolates were characterized from 10 wild Ipomoea species, Hewittia sublobata or Lepistemon owariensis (family Convolvulaceae in Uganda and compared with 34 local SPCSV isolates infecting sweetpotatoes. All isolates belonged to the East African (EA strain of SPCSV and contained RNase3 and p7, but p22 was not detected in six isolates. The three genes showed only limited genetic variability and the proteins were under purifying selection. SPCSV isolates lacking p22 synergized with Sweet potato feathery mottle virus (SPFMV, genus potyvirus; Potyviridae and caused severe symptoms in co-infected sweetpotato plants. One SPCSV isolate enhanced accumulation of SPFMV, but no severe symptoms developed. A new whitefly-transmitted virus (KML33b encoding an RNase3 homolog (<56% identity to SPCSV RNase3 able to suppresses sense-mediated RNA silencing was detected in I. sinensis. CONCLUSIONS/SIGNIFICANCE: SPCSV isolates infecting wild species and sweetpotato in Uganda were genetically undifferentiated, suggesting inter-species transmission of SPCSV. Most isolates in Uganda contained p22, unlike SPCSV isolates characterized from other countries and continents. Enhanced accumulation of SPFMV and increased disease severity were found to be uncoupled phenotypic outcomes of RNase3-mediated viral synergism in

  9. On-farm evaluation of sweetpotato varieties in Zanzibar | Saleh ...

    African Journals Online (AJOL)

    On-farm evaluation of new crop varieties is crucial for speedy adoption of the materials by farmers. In Zanzibar, many experiments on sweetpotato (Ipomea batatas) have been conducted, but mostly on-station, hence, farmers\\' participation was limited. To improve farmers' adoption of new technologies including acceptance ...

  10. Hábito alimentario y descripción morfológica de la larva de dos especies de pega-pega (Helcystogramma spp. y Lygropia tripunctata (Fabricius en boniato (Ipomoea batatas (L. Lam.

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    Alfredo Morales Rodríguez

    2015-08-01

    Full Text Available Alimentary habit and morphological description of the larva of two species of leaf folder (Helcystogramma spp. and Lygropia tripunctata (Fabricius of sweet potato (Ipomoea batatas L. Lam. Too little are known about the majority of defoliators insects of the sweet potato, as its the case of lepidopterous Helcystogramma sp and Lygropia tripunctata. The objectives of present investigation are to know morphologic aspects and alimentary habit of the larva of these species. The work was done at Institute Research of Tropical Root (INIVIT. Insects were studied by direct observation in two fields of sweet potato. The larva of Helcystogramma sp has the head, three thoracic segments and three pairs of paws of black color, while the larva of L. tripunctata has the head of yellow color and its has the body green-bluish translucent. The larva of Helcystogramma sp glues the leaf with silk threads and when its feed leave the inferior epidermis or superior intact. L.tripunctata glues several leaf during its larval status and its does not eat the part of the leaf that it glues.

  11. Analysis of sphingolipids in potatoes (Solanum tuberosum L.) and sweet potatoes (Ipomoea batatas (L.) Lam.) by reversed phase high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS).

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    Bartke, Nana; Fischbeck, Anne; Humpf, Hans-Ulrich

    2006-12-01

    Ceramides and glucocerebrosides of potatoes (Solanum tuberosum L.) and sweet potatoes (Ipomoea batatas (L.) Lam.) were analyzed using RP-HPLC-ESI-MS/MS. Ceramides and glucocerebrosides containing the three different long-chain bases 4,8-sphingadienine (d18:2(delta4,delta8)), 4-hydroxy-8-sphingenine (t18:1(delta8)), and 8-sphingenine (d18:1(delta8)) acylated to saturated and unsaturated hydroxy- and nonhydroxy fatty acids with 16-26 carbon atoms were detected. For ceramides and glucocerebrosides 4,8-sphingadienine (d18:2(delta4,delta8)) was found as the major long-chain base, with lesser amounts of 4-hydroxy-8-sphingenine (t18:1(delta8)) and 8-sphingenine (d18:1(delta8)). 2-(Alpha-)hydroxypalmitic acid (C16:0h) was the major fatty acid, which was found to be acylated to the long-chain bases. For quantification of these compounds, an RP-HPLC-ESI-MS/MS method with an "echo-peak"-technique simulating internal standard injection was developed. The analyzed samples of potatoes and sweet potatoes showed amounts of approximately 0.1-8 microg/kg single ceramides and amounts up to 500 microg/kg glucocerebrosides, with C16:0h-glucosyl-4,8-sphingadienine as the major component.

  12. Molecular characterization of tocopherol biosynthetic genes in sweetpotato that respond to stress and activate the tocopherol production in tobacco.

    Science.gov (United States)

    Ji, Chang Yoon; Kim, Yun-Hee; Kim, Ho Soo; Ke, Qingbo; Kim, Gun-Woo; Park, Sung-Chul; Lee, Haeng-Soon; Jeong, Jae Cheol; Kwak, Sang-Soo

    2016-09-01

    Tocopherol (vitamin E) is a chloroplast lipid that is presumed to be involved in the plant response to oxidative stress. In this study, we isolated and characterized five tocopherol biosynthetic genes from sweetpotato (Ipomoea batatas [L.] Lam) plants, including genes encoding 4-hydroxyphenylpyruvate dioxygenase (IbHPPD), homogentisate phytyltransferase (IbHPT), 2-methyl-6-phytylbenzoquinol methyltransferase (IbMPBQ MT), tocopherol cyclase (IbTC) and γ-tocopherol methyltransferase (IbTMT). Fluorescence microscope analysis indicated that four proteins localized into the chloroplast, whereas IbHPPD observed in the nuclear. Quantitative RT-PCR analysis revealed that the expression patterns of the five tocopherol biosynthetic genes varied in different plant tissues and under different stress conditions. All five genes were highly expressed in leaf tissues, whereas IbHPPD and IbHPT were highly expressed in the thick roots. The expression patterns of these five genes significantly differed in response to PEG, NaCl and H2O2-mediated oxidative stress. IbHPPD was strongly induced following PEG and H2O2 treatment and IbHPT was strongly induced following PEG treatment, whereas IbMPBQ MT and IbTC were highly expressed following NaCl treatment. Upon infection of the bacterial pathogen Pectobacterium chrysanthemi, the expression of IbHPPD increased sharply in sweetpotato leaves, whereas the expression of the other genes was reduced or unchanged. Additionally, transient expression of the five tocopherol biosynthetic genes in tobacco (Nicotiana bentamiana) leaves resulted in increased transcript levels of the transgenes expressions and tocopherol production. Therefore, our results suggested that the five tocopherol biosynthetic genes of sweetpotato play roles in the stress defense response as transcriptional regulators of the tocopherol production. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  13. Utilization effect of cassava (Manihot esculenta and potatoe (Ipomoea batatas on pigmentation, carotenoids pull on egg yolk and performance yield hens Influencia de harinas de yuca y de batata sobre pigmentación, contenido de carotenoides en la yema y desempeño productivo de aves en postura

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    Ceballos L Hernán

    2006-09-01

    Full Text Available

    Utilization effect of cassava (Manihot esculenta and potatoe (Ipomoea batatas on pigmentation, carotenoids pull on egg yolk and performance yield hens. Eight diets (T1:without pigment, T2: commercial pigment, T3: cassava meal 15%, T4: cassava meal 30%, T5: sweet potato meal 15%, T6: sweet potato meal 30%, T7: cassava and sweet potato meal 15% each, T8: cassava and sweet potato meal 25% each in a randomized design using four replicates of three hens each during seven experimental periods were evaluated. Diets were iso-energetics (2.9 kcal/g and iso-proteis (14.5% CP. Carotenoid concentrations (UV-visible chromatography had differences (P<0.01 among diets. Pigmentation (Fan Roche and carotenoid concentration in egg yoks to come from cassava and sweet potato diets did not show differences (P>0.05 A comparison with egg yolk to come from test diets significant differences (P<0.01 were detected. Diet with commercial pigment showed the highest pigmentation and carotenoid concentration in egg yolk. The other hand, there saw not differences (P>0.05. A comparison with egg yolk to come from test diets significant differences (P<0.01 were detected. Diet with commercial pigment showed the highest pigmentation and carotenoid concentration in egg yolk. The other hand, there saw not differences (P>0.05 between egg yolk to come from diet without pigment and cassava and sweet potato diets. Lutein, -carotene, -carotene, and 9-cis-- carotene were the

  14. Influência da adição da fécula de batata-doce (Ipomoea batatas L. sobre a viscosidade do permeado de soro de queijo Influence of sweet potato starch at permeate whey viscosity

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    Rafael Leite Pinto de Andrade

    2002-12-01

    Full Text Available A viscosidade é um parâmetro importante na aceitabilidade de bebidas lácteas. O presente trabalho teve como objetivo principal fornecer dados relativos ao comportamento da viscosidade uma base de permeado de soro de queijo adicionada de fécula de batata-doce, dados que poderão ser utilizados na elaboração de uma nova bebida láctea. A fécula de batata-doce utilizada apresentou um grau de gelatinização de 72,64%, umidade média de 7,88% e a seguinte composição centesimal média em base seca: 82,59% de amido, 9,33% de fibras dietéticas totais, 4,90% de proteínas, 2,08% de cinzas e 1,11% de lipídios. O permeado de soro foi adicionado a três diferentes concentrações de fécula e submetido a diferentes tratamentos térmicos. A viscosidade mais adequada, em função dos dados obtidos junto aos produtos comerciais, estaria no intervalo de 45mPa.s a 70mPa.s. Assim sendo, a porcentagem de 6% de fécula de batata-doce e um tratamento térmico de 90ºC por cerca de 5 minutos mostrou-se ser o mais adequado. O fluido obtido apresentou um comportamento pseudoplástico.Viscosity is an important parameter of milky drinks acceptability. This work supply data of the viscosity properties of permeate whey plus sweet potato starch basis; these data will may be used at development of a new milky beverage. The used sweet potato starch presented 72.64% of gelatinization, 7.88% of humidity and the following dry composition: 82.59% starch, 9.33% total dietary fibres, 4.90% proteins, 2.08% ashes and 1.10% lipids. The permeate whey had mixed at three different percentages of potato starch and had submitted at various thermal treatments. The most appropriate viscosity, in accord of commercial products, it would be in the interval of 45mPa.s to 70mPa.s. Therefore, the percentage of 6% of sweet potato starch and a thermal treatment of 90ºC for about 5 minutes it was shown to be the most appropriate. The fluid presented a pseudoplastic properties.

  15. Molecular cloning and functional analysis of the ortho-hydroxylases of p-coumaroyl coenzyme A/feruloyl coenzyme A involved in formation of umbelliferone and scopoletin in sweet potato, Ipomoea batatas (L.) Lam.

    Science.gov (United States)

    Matsumoto, Seitaro; Mizutani, Masaharu; Sakata, Kanzo; Shimizu, Bun-Ichi

    2012-02-01

    Ortho-hydroxylation of cinnamates is a key step in coumarin biosynthesis in plants. Ortho-hydroxylated cinnamates undergo trans/cis isomerization of the side-chain and then lactonization to form coumarins. Sweet potato [Ipomoea batatas (L.) Lam.] accumulates umbelliferone and scopoletin after biotic and abiotic stresses. To elucidate molecular aspects of ortho-hydroxylation involved in umbelliferone formation in sweet potato, isolation and characterization of cDNAs encoding 2-oxoglutarate-dependent dioxygenases (2OGD) was performed from sweet potato tubers treated with a chitosan elicitor. Five cDNAs (designated as Ib) encoding a protein of 358 amino acid residues were cloned, and these were categorized into two groups, Ib1 and Ib2, based on their amino acid sequences. Whether the recombinant Ib proteins had any enzymatic activity toward cinnamates was examined. Ib1 proteins exhibited ortho-hydroxylation activity toward feruloyl coenzyme A (CoA) to form scopoletin (K(m)=~10 μM, k(cat)=~2.7s(-1)). By contrast, Ib2 proteins catalyzed ortho-hydroxylation of feruloyl-CoA (K(m)=7.3-14.0 μM, k(cat)=0.28-0.55 s(-1)) and also of p-coumaroyl-CoA (K(m)=6.1-15.2 μM, k(cat)=0.28-0.64 s(-1)) to form scopoletin and umbelliferone, respectively. Fungal and chitosan treatments increased levels of umbelliferone and its glucoside (skimmin) in the tubers, and expression of the Ib2 gene was induced concomitantly. Copyright © 2011 Elsevier Ltd. All rights reserved.

  16. Suppression of the invasive plant mile-a-minute (Mikania micrantha) by local crop sweet potato (Ipomoea batatas) by means of higher growth rate and competition for soil nutrients.

    Science.gov (United States)

    Shen, Shicai; Xu, Gaofeng; Clements, David Roy; Jin, Guimei; Chen, Aidong; Zhang, Fudou; Kato-Noguchi, Hisashi

    2015-01-28

    There are a variety of ways of increasing crop diversity to increase agricultural sustainability and in turn having a positive influence on nearby natural ecosystems. Competitive crops may provide potent management tools against invasive plants. To elucidate the competitive mechanisms between a sweet potato crop (Ipomoea batatas) and an invasive plant, mile-a-minute (Mikania micrantha), field experiments were carried out in Longchuan County of Yunnan Province, Southwest China, utilizing a de Wit replacement series. The trial incorporated seven ratios of sweet potato and mile-a-minute plants in 25 m(2) plots. In monoculture, the total biomass, biomass of adventitious root, leafstalk length, and leaf area of sweet potato were all higher than those of mile-a-minute, and in mixed culture the plant height, branch, leaf, stem node, adventitious root, flowering and biomass of mile-a-minute were suppressed significantly (P sweet potato was less than 1.0 in mixed culture, indicating that intraspecific competition was less than interspecific competition. The competitive balance index of sweet potato demonstrated a higher competitive ability than mile-a-minute. Except pH, other soil nutrient contents of initial soil (CK) were significantly higher than those of seven treatments. The concentrations of soil organic matter, total N, total K, available N, available P, available K, exchange Ca, exchange Mg, available Mn, and available B were significantly greater (P sweet potato monoculture soil, and were reduced by the competition of sweet potato in the mixture. Evidently sweet potato has a competitive advantage in terms of plant growth characteristics and greater absorption of soil nutrients. Thus, planting sweet potato is a promising technique for reducing infestations of mile-a-minute, providing weed management benefits and economic returns from harvest of sweet potatoes. This study also shows the potential value of replacement control methods which may apply to other crop

  17. Effects of feeding sweet potato (Ipomoea batatas) vines as a supplement on feed intake, growth performance, digestibility and carcass characteristics of Sidama goats fed a basal diet of natural grass hay.

    Science.gov (United States)

    Megersa, Tadesse; Urge, Mengistu; Nurfeta, Ajebu

    2013-02-01

    The objective of this study was to investigate the effects of substituting sweet potato [Ipomoea batatas (L) Lam] vines for concentrate on growth performance, digestibility, and carcass characteristics. Thirty yearling bucks (15.3 ± 1.64 kg) were assigned into six treatments in a randomized complete block design: natural grass hay alone (T1) or supplemented with 100 % sweet potato vines (SPV) (T2), 65 % SPV + 35 % concentrate (T3), 35 % SPV + 65 % concentrate (T4), and 100 % concentrate (T5) on dry matter (DM) basis. Supplemented goats (T2, T3, T4, and T5) consumed higher (p < 0.001) total DM (553, 567, 505, and 515 g/day), respectively, when compared to the nonsupplemented (T1) goats (349 g/day). The crude protein (CP) intake (32.0, 48.6, 54.7, and 69.2 g/day) increased with increasing levels of the concentrate in the diet for T2, T3, T4, and T5, respectively. The DM digestibility in T2, T3, T4, and T5, respectively, was higher (P < 0.01) (0.69, 0.72, 0.72, and 0.74) than in T1 (0.56). Apparent digestibility of CP was observed to be higher (P < 0.001) in T3, T4, T5 (0.78, 0.83, and 0.88) when compared to the bucks in T2 (0.60). Higher (P < 0.001) daily weight gain (31.2, 46.4, 48.6, and 47.6 g/day) were recorded for T2, T3, T4, and T5, respectively, whereas the nonsupplemented goats lost weight (-19.5 g/day). Slaughter weight, empty body weight, hot carcass weight, dressing percentage, rib-eye muscle area, and total edible offals were higher (P < 0.05) in supplemented goats compared with nonsupplemented ones. Therefore, it could be concluded that sweet potato vine can replace the conventional concentrate and could be fed with poor quality hay to prevent body weight loss of animal in the absence of other feed supplements.

  18. Characterisation and performance evaluation of twenty accessions of sweet potato (Ipomoea batatas (L.) Lam.) in the coastal savannah agro-ecological zone of Ghana

    International Nuclear Information System (INIS)

    Sossah, F.L.

    2012-01-01

    This study assessed the genetic diversity, using morphological and protein markers, as well as incidence of viral diseases among 20 accessions of sweet potato. Morphological analysis was carried out using 47 traits while protein analysis was performed with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Sample plants collected from the field were tested for viruses using nitrocellulose membrane enzyme-linked immuno-sorbent assay (NCM-ELISA) and PCR for a DNA virus (Sweet potato Leaf curl virus). Scions from symptomatic plants were grafted onto Ipomoea setosa, a nearly universal indicator plant for sweet potato viruses, and leaves were tested using NCM-ELISA. From the cluster analysis, the accessions were grouped into two clusters at a genetic similarity index of 61.6%. The accessions ER001 and HMA2 were shown to be possible duplicates. Accession US 020 recorded the highest total root yield and harvest index of 56.32 t/ha and 57.11% respectively. Similarly, two pairs of accession SA/BNARI and UE 007 as well as HMA I and US 029 were identified as duplicates based on their protein banding patterns. While, three entries, LOCAL I, US 004 and UK/BNARI were also identified as a single entity based on their banding patterns for leaf and tuber proteins. Symptoms observed in the field varied from vein clearing, chlorosis, leaf reduction to stunting and the highest index of symptom severity for only diseased plants (ISSdp) was 3.63 recorded for US 029. The highest disease incidence (100%) was recorded at 11 WAP and 12 WAP. NCM-ELISA test detected eight viruses out of ten, with SA/BNARI exhibiting all the eight viruses. Mixed infections ranged from duplet to octaplet. The indicator plant I. setosa grafted with scions from sweet potato showed clear symptoms similar to those observed on the field. PCR test using SPG3/SPG4 primers detected SPLCV in the UK/BNARI accessions. Based on the morphological characters as well as response to virus infection, two

  19. Composición mineral y comparación de raíces reservantes de variedades de batatas (Ipomoea batatas (L. Lam. cultivadas en la isla de La Palma, España

    Directory of Open Access Journals (Sweden)

    Adriana Isabel Montes Hernández

    2010-06-01

    Full Text Available Los minerales Na, K, Ca, Mg, Fe, Cu, Zn y Mn fueron determinados, mediante espectrofotometría de absorción atómica, en raíces de 15 variedades de batatas cultivadas en La Palma (De papa o maní, Parado, Cubana, Cubana de baselo gordo, Cubana de hoja redonda, Blanquita, Saucero o Lanzarotero, Pata de Gallo, Padrón de Seda, Alicantina, Amarillo de Año, De Lanzarote, Matojo, Matojo Fino y Rajada; las 5 primeras de cultivo corto y las 10 restantes de cultivo largo. Se realizó análisis de varianza y prueba de rango múltiple de Duncan a los datos de las variables estudiadas. Se calcularon los coeficientes de correlación de Pearson (r para establecer grados de relación de los minerales entre las variedades; prueba de t de Student para comparar clasificando a las muestras según ciclo de recolección (Temprana y Tardía; análisis factorial para interpretar las relaciones existentes a través factores y análisis discriminantes (de paso a paso con criterio de selección la lambda de Wilks (λ e introduciendo todas las variables validados a través de análisis de validación cruzada. El elemento mineral (en mg/kg presente en mayor concentración en la totalidad de las muestras fue K (2086 ± 418, seguido de Ca (974 ± 486 y Mg (244 ± 70, siendo el Zn el menos presente (1,12 ± 0,61. Las variedades con mayor contenido mineral fueron Alicantina (Ca: 1809 ± 327 y Cu: 2,97 ± 0,23 y Padrón de Seda (Mg: 435 ± 46 y Mn: 3,08 ± 0,49 y la de menor aporte fue Parado (Cu, Mn, Ca, K y Mg. Las variedades Tardías presentaron mayores concentraciones de Cu, Fe, Mn, Ca, K, Mg y menores de Zn y Na en relación a las variedades Tempranas (p < 0,05. Se evidenció que las concentraciones de Cu, Zn y Na presentaron alto número de correlaciones significativas. El análisis factorial permitió deducir que Mg, K y Fe son las variables que permiten caracterizar el sistema perdiendo un mínimo de información total. El porcentaje de casos originales

  20. Registration of Adu and Barkume: Improved Sweet Potato ( Ipomoea ...

    African Journals Online (AJOL)

    Two improved sweet potato (Ipomoea batatas) varieties, namely, Adu (Cuba-2) and Barkume (TIS-8250-2) were developed by Root and Tuber Crops Improvement Program and approved by the National Variety Releasing Committee in 2007. The performances of the varieties were evaluated at four locations in the eastern ...

  1. Genetic Diversity of Local and Introduced Sweet Potato [Ipomoea ...

    African Journals Online (AJOL)

    This study was therefore conducted to estimate the genetic diversity of 114 Sweet potato [Ipomoea batatas (L.) Lam] accessions obtained from Nigeria, Asia, Latin America and Local collections along with two improved varieties. Accessions were planted in 2012/13 cropping season at Haramaya University, eastern Ethiopia ...

  2. Evaluation of the performance of improved sweet potato ( Ipomoea ...

    African Journals Online (AJOL)

    Evaluation of the performance of improved sweet potato ( Ipomoea batatas L. LAM) varieties in Bayelsa State, Nigeria. ... There was incidence of diseases but that of insects was low. For fresh root phenotypic characteristics, Ex-Igbariam and 199004-2 had yellow flesh, indicative of the presence of vitamin A precursor.

  3. Etude comparative de la digestibilité in vivo de l'herbe de guinée (Panicum maximum Jacq. associée aux feuilles et tiges de patate douce (Ipomoea batatas (L. Lam (régime Panipo ou à l'herbe de lait (Euphorbia heterophylla L. (régime Paneuph chez le lapin (Oryctolagus cuniculus L. et le cobaye (Cavia porcellus L.

    Directory of Open Access Journals (Sweden)

    Kouakou, NGDV.

    2016-01-01

    Full Text Available Digestibility of Guinea grass (Panicum maximum Jacq. associated with sweet potato leaves and stems (Ipomoea batatas (L. Lam (Panipo diet or poison milk (Euphorbia heterophylla L. (Paneuph diet in Rabbits (Oryctolagus cuniculus L. and in Guinea Pigs (Cavia porcellus L.. A comparative study of in vivo digestibility of Guinea grass (Panicum maximum Jacq. associated with sweet potato leaves and stems (Ipomoea batatas (L. Lam (Panipo diet or with poison milk (Euphorbia heterophylla L. (Paneuph diet was conducted involving 16 rabbits and 16 weaned guinea pigs fed ad libitum. Paneuph was significantly better ingested (P0.05 for guinea pigs. Poison milk was better ingested than the leaves and stems of sweet potato (P0.05. The ADC of DM, OM and MAT of Paneuph exceeded 80% and were higher than those of Panipo in rabbits and guinea pigs (P<0.05. Based on the results obtained, the effects of the distribution of the Guinea grass associated with the poison milk during rabbits growing have to be further studied before recommending this new diet to farmers in areas where poison milk and Guinea grass are present and rabbit breeding enough developed.

  4. Genetic analysis and association of simple sequence repeat markers with storage root yield, dry matter, starch and β-carotene content in sweetpotato

    Science.gov (United States)

    Yada, Benard; Brown-Guedira, Gina; Alajo, Agnes; Ssemakula, Gorrettie N.; Owusu-Mensah, Eric; Carey, Edward E.; Mwanga, Robert O.M.; Yencho, G. Craig

    2017-01-01

    Molecular markers are needed for enhancing the development of elite sweetpotato (Ipomoea batatas (L.) Lam) cultivars with a wide range of commercially important traits in sub-Saharan Africa. This study was conducted to estimate the heritability and determine trait correlations of storage root yield, dry matter, starch and β-carotene content in a cross between ‘New Kawogo’ × ‘Beauregard’. The study was also conducted to identify simple sequence repeat (SSR) markers associated with these traits. A total of 287 progeny and the parents were evaluated for two seasons at three sites in Uganda and genotyped with 250 SSR markers. Broad sense heritability (H2) for storage root yield, dry matter, starch and β-carotene content were 0.24, 0.68, 0.70 and 0.90, respectively. Storage root β-carotene content was negatively correlated with dry matter (r = −0.59, P < 0.001) and starch (r = −0.93, P < 0.001) content, while storage root yield was positively correlated with dry matter (r = 0.57, P = 0.029) and starch (r = 0.41, P = 0.008) content. Through logistic regression, a total of 12, 4, 6 and 8 SSR markers were associated with storage root yield, dry matter, starch and β-carotene content, respectively. The SSR markers used in this study may be useful for quantitative trait loci analysis and selection for these traits in future. PMID:28588391

  5. Mixed Infections of Four Viruses, the Incidence and Phylogenetic Relationships of Sweet Potato Chlorotic Fleck Virus (Betaflexiviridae) Isolates in Wild Species and Sweetpotatoes in Uganda and Evidence of Distinct Isolates in East Africa.

    Science.gov (United States)

    Tugume, Arthur K; Mukasa, Settumba B; Valkonen, Jari P T

    2016-01-01

    Viruses infecting wild flora may have a significant negative impact on nearby crops, and vice-versa. Only limited information is available on wild species able to host economically important viruses that infect sweetpotatoes (Ipomoea batatas). In this study, Sweet potato chlorotic fleck virus (SPCFV; Carlavirus, Betaflexiviridae) and Sweet potato chlorotic stunt virus (SPCSV; Crinivirus, Closteroviridae) were surveyed in wild plants of family Convolvulaceae (genera Astripomoea, Ipomoea, Hewittia and Lepistemon) in Uganda. Plants belonging to 26 wild species, including annuals, biannuals and perennials from four agro-ecological zones, were observed for virus-like symptoms in 2004 and 2007 and sampled for virus testing. SPCFV was detected in 84 (2.9%) of 2864 plants tested from 17 species. SPCSV was detected in 66 (5.4%) of the 1224 plants from 12 species sampled in 2007. Some SPCSV-infected plants were also infected with Sweet potato feathery mottle virus (SPFMV; Potyvirus, Potyviridae; 1.3%), Sweet potato mild mottle virus (SPMMV; Ipomovirus, Potyviridae; 0.5%) or both (0.4%), but none of these three viruses were detected in SPCFV-infected plants. Co-infection of SPFMV with SPMMV was detected in 1.2% of plants sampled. Virus-like symptoms were observed in 367 wild plants (12.8%), of which 42 plants (11.4%) were negative for the viruses tested. Almost all (92.4%) the 419 sweetpotato plants sampled from fields close to the tested wild plants displayed virus-like symptoms, and 87.1% were infected with one or more of the four viruses. Phylogenetic and evolutionary analyses of the 3'-proximal genomic region of SPCFV, including the silencing suppressor (NaBP)- and coat protein (CP)-coding regions implicated strong purifying selection on the CP and NaBP, and that the SPCFV strains from East Africa are distinguishable from those from other continents. However, the strains from wild species and sweetpotato were indistinguishable, suggesting reciprocal movement of SPCFV

  6. Absorção e infiltração de água por raízes de batata-doce, através de ferimentos durante a lavagem Water absorption and infiltration in sweet-potato wound roots during washing

    Directory of Open Access Journals (Sweden)

    Adonai Gimenez Calbo

    2000-09-01

    Full Text Available Raízes intactas e segmentos transversais de batata-doce (Ipomea batatas L. Lam foram totalmente ou parcialmente imersos em água. Raízes ou segmentos parcialmente imersos ficaram com menos de 10% da superfície externa fora da água, em um suspiro. Nestes ensaios a pressão da atmosfera interna das raízes parcialmente imersas manteve-se apenas alguns milímetros inferior a pressão atmosférica. Nas raízes totalmente imersas a pressão reduziu-se quase linearmente até mais de 1m de coluna de água e depois voltou a aumentar lentamente. Nas raízes intactas ou nos seus segmentos transversais totalmente imersos, houve maior infiltração de água nos volumes intercelulares do que nas raízes ou segmentos parcialmente imersos. Nas raízes intactas praticamente houve apenas absorção de água através das paredes e membranas celulares. Nos segmentos a infiltração de água através dos volumes intercelulares foi dominante. Considerando-se as dimensões transversais dos volumes intercelulares, sugere-se que partículas como bactérias e esporos possam ser arrastados para o interior órgão pela infiltração de água durante a lavação dos tecidos mecânicamente danificados.Intact roots and transversal root segments of sweet-potato (Ipomea batatas L. Lam were partially or completely submerged in water. In partially submerged roots less than 10% of the dermal surface remained exposed to air through a vent. Internal atmosphere pressure in partially submerged roots remained a few millimeters bellow the atmospheric pressure, while a much larger and nearly linear pressure reduction occurred in completely submerged roots. This linear phase caused a pressure reduction larger than 1m of water column. After that, the pressure started to rise slowly. The observed pressure reduction was smaller than the one observed in the constant pressure manometry procedure where the roots were sealed with epoxy resin, which precluded any water infiltration and

  7. Effects of land use changes on kinetics of potassium release in sweetpotato garden soils of the highlands, Papua New Guinea

    Science.gov (United States)

    Rajashekhar Rao, B. K.

    2014-09-01

    The present study attempts to employ K release parameters to identify soil quality degradation due to changed land use pattern in sweetpotato (Ipomoea batatas (L.) Lam) gardens of Highlands of Papua New Guinea. Soils with widely differing exchangeable and non-exchangeable K contents were successively extracted 569 h in 0.01 M CaCl2 and K release data was fitted to four mathematical models: first order, power, parabolic diffusion and Elovich equations. Results showed two distinct parts in the K release curves and 58-80% of total K were released to solution phase within 76 h (first 5 extractions) with 20-42% K released in the later parts (after 76 h). Soils from older gardens which were subjected to intensive and prolonged land use showed significantly (P < 0.05) lower cumulative K release potential than the gardens which are recently brought to cultivation (new gardens). Among four equations, first order and power equations best described the K release pattern and the constant b, an index of K+ release rates, ranged from 0.005-0.008 mg kg-1h-1 in first order model, and was between 0.14 and 0.83 mg kg-1h-1 in power model for the soils. In the non-volcanic soils, model constant b values were significantly (P < 0.05) higher than the volcanic soils thus indicative of vulnerability of volcanic soils to K deficiency. The food garden soils need management interventions either through improved fallow management or through mineral fertilizers plus animal manures to sustain productivity.

  8. Batata-doce [Ipomoea batatas (L.) Lam.] nas roças e quintais do litoral paulista: diversidade genética morfoagronômica, com base em morfometria geométrica, descritores e produção de bioetanol

    OpenAIRE

    Hendrie Ferreira Nunes

    2016-01-01

    A batata-doce é uma das fontes de alimento mais importantes do mundo, sendo muito cultivada no Brasil por agricultores tradicionais. Os caiçaras, habitantes do litoral do Sudeste brasileiro, têm exercido enorme influência na manutenção da diversidade genética em muitas culturas, incluindo a batata-doce. Como os processos de mudanças socioeconômicas e agrícolas nesta região são muito rápidos e têm provocado alterações constantes nesses sistemas agroecológicos, pretendeu-se capturar o nível de ...

  9. The perspective of sweetpotato chlorotic stunt virus in sweetpotato ...

    African Journals Online (AJOL)

    The virus is transmitted by the whitefly species, Bemisia tabaci and Trialeurodes abutilonea, in a semi-persistent fashion. ... yields being small, and the major effect of SPCSV in constraining the yields of sweetpotato is perhaps through preventing the cultivation of high yielding but SPVD-susceptible sweetpotato cultivars.

  10. Exploitation of genetic potential of sweetpotato for end-user traits ...

    African Journals Online (AJOL)

    La patate douce (Ipomoea batatas Lam) est un aliment de base à l'échelle mondiale, mais c'est une ressource sousutilisée au Ghana en raison du manque de cultivars préféré par les consommateurs. Il est nécessaire de développer des cultivars de patate douce de type de base qui sont préférés par les consommateurs ...

  11. Produção da batata-doce adubada com esterco bovino e biofertilizante

    OpenAIRE

    Oliveira,Ademar Pereira de; Barbosa,Arthur Hennys Diniz; Cavalcante,Lourival Ferreira; Pereira,Walter Esfraim; Oliveira,Arnaldo Nonato Pereira de

    2007-01-01

    Objetivou-se avaliar a produção da batata-doce (Ipomoea batatas (L.) Lam.) adubada com esterco bovino e biofertilizante, conduziu-se um experimento no período de Janeiro a Junho de 2004, em NEOSSOLO REGOLÍTICO, psamítico típico, na Universidade Federal da Paraíba, em Areia-PB. O delineamento experimental empregado foi de blocos casualisados, com quatro repetições com os tratamentos distribuídos em arranjo fatorial 6 x 2, sendo seis doses de esterco bovino (0, 10, 20, 30, 40, 50 t ha-1) e ausê...

  12. Embryogenesis in sweet potato, Ipomea batatas (L.) Lam

    International Nuclear Information System (INIS)

    Sonnino, A.; Mini, P.

    1997-01-01

    Sweet potato (Ipomoea batatas (L.) Lam) ranks sixth among the cultivated crops of the world. In fact, it represents a major staple food in many tropical countries. Recently this crop has been proposed as a source of starch for industrial utilization. Somatic embryogenesis could prove useful as an alternative to traditional propagation by cuttings, which is labour intensive and can transmit diseases. Somatic embryos are reported to originate from single cells, so that, if regenerated from mutagenized tissues, should give rise to solid mutants. 2 refs

  13. Transmission of Sweet Potato Leaf Curl Virus by Bemisia tabaci

    Science.gov (United States)

    Sweetpotato, Ipomoea batatas (L.) Lam. (Solanales: Convolvulaceae), is an important world food crop, and Asia is the focal production region. Because it is vegetatively propagated, sweetpotato is especially prone to accumulate infections by several viruses. Sweet potato leaf curl virus (SPLCV) (ss...

  14. Transient expression of β-glucuronidase in recalcitrant Ugandan ...

    African Journals Online (AJOL)

    Sweetpotato (Ipomoea batatas Lam.) has high potential to contain hunger, malnutrition and poverty in sub-Saharan Africa (SSA), since it gives early yield with few inputs. However, productivity of the crop in Africa is very low due to various challenges, such as severe viral diseases and increasing attacks by sweetpotato ...

  15. Possibilities of sweet potato [ Ipomoea batatas (L.) Lam] value chain ...

    African Journals Online (AJOL)

    Sweet potato is one of the most important food security promoted root crops in the world, especially in sub-Saharan Africa. Unfortunately, the crop is still neglected and underutilized in Benin Republic. To establish baseline data for its better utilization for upgrading its value chain, 10 selected local varieties (01 cream, ...

  16. Genetic Fingerprinting of Sweet Potato [ Ipomoea batatas (L.) Lam ...

    African Journals Online (AJOL)

    Sweet potato is an important staple crop and many varieties have been released into farmers' fields in Nigeria, but no reliable means in tracking their identity, thus causing multiple naming of these varieties among farmers. The objective of the study is to establish objectively and reliable means of identifying released, local ...

  17. Genetic Fingerprinting of Sweet Potato [Ipomoea batatas (L.) Lam ...

    African Journals Online (AJOL)

    Prof. Ogunji

    The use of morphological characters to study the variability in sweet potato and hybrid progenies have been reported and observed the inert-relationship of different characters such as yield in association with length of petiole, number of tubers and the mean tuber weight (Thankamma et al. 1990,. Arslanoglu et al. 2011; and ...

  18. Diversity analysis of sweet potato (Ipomoea batatas [L.] Lam ...

    African Journals Online (AJOL)

    aghomotsegin

    2014-02-05

    Feb 5, 2014 ... matrix of component loadings. The eigenvalues and their associated eigenvectors, the correlation matrix are used to reduce the number of variables in the statistical analyses. (Daulfrey, 1976). A graphical display of the genetic relationships was also computed by principal coordinate analysis using the.

  19. Genetic Fingerprinting of Sweet Potato [Ipomoea batatas (L.) Lam ...

    African Journals Online (AJOL)

    Prof. Ogunji

    Isozyme data analysis revealed four clusters and insignificant correlations were observed between the isozyme and morphological analyses. .... and inner skin colour and the flesh colour. Data collected were based on International .... using the numerical taxonomy and multivariate analysis system (NTSYS-PC), Version 2.1 ...

  20. Possibilities of sweet potato [Ipomoea batatas (L.) Lam] value chain ...

    African Journals Online (AJOL)

    FAOUZIATH

    2016-03-30

    Mar 30, 2016 ... (2014) in Martinique. According to Trinidad et al. (2013), dietary fibers are important in preventing cardiovascular diseases and diabetes mellitus and they are also efficient in reducing the incidences of colon cancer and certain digestive diseases (Ingabire and. Hilda, 2011). Carbohydrate is represented in ...

  1. (Ipomoea batatas) leaves on growth performance and nutrient ...

    African Journals Online (AJOL)

    Dr Abonyi

    2012-02-21

    Feb 21, 2012 ... (2005) indicated several advantages rabbit has over other livestock in the tropics ... Dietary treatments ratio of pelletized concentrate feed (PCF) and sweet potato leaves (SPL). Diet. Treatment. T1. T2. T3. T4 ... analysis of variance using the PROC ANOVA statement as found in the SAS computer software ...

  2. Protective Effect of Purple Sweet Potato (Ipomoea batatas Linn ...

    African Journals Online (AJOL)

    University, Korea and a voucher specimen (IB-. KU2013) has been kept in our laboratory. Herbarium, Konkuk University, Korea for future reference. To obtain the I. ..... the Regional Strategic Planning Technology. Development Program funded by the Ministry of. Knowledge Economy of the Korean government, as well as by ...

  3. Reaction of sweetpotato clones to virus disease and their yield ...

    African Journals Online (AJOL)

    Sweetpotato virus disease (SPVD) caused by dual infection of sweetpotato chlorotic stunt virus (SPCSV) and sweetpotato featherly mottle virus (SPFMV) is a major constraint to sweetpotato production in Uganda, whose infestation often necessitates instituting control measures. Although among the available control ...

  4. Assessment of the Extent of Adoption of Sweetpotato Production ...

    African Journals Online (AJOL)

    The constraints to increased adoption of the technology were scarcity of land, difficulty in integrating sweetpotato production technology into existing production system, low consumer preference associated with sweetpotato products, lack of market, unavailability of sweetpotato vines, high cost of available sweetpotato vines ...

  5. Integrated pest management for sweetpotato in Eastern Africa

    OpenAIRE

    Smit, N.

    1997-01-01

    Sweetpotato is an important crop in Eastern Africa. Sweetpotato weevils ( Cylas puncticollis Boheman and C. brunneus Fabricius; Coleoptera: Apionidae) cause damage to roots and vines
    throughout the crop's production area. Other insect pests of sweetpotato are of regional importance. The aim of the research project was to gain insight in the biology and ecology of sweetpotato weevils and, based on this ...

  6. Species diversity and activity of parasitoids of the sweetpotato ...

    African Journals Online (AJOL)

    The species range, activity and relative abundance of parasitoids attacking the sweetpotato butterfly, Acraea acerata Hew. (Lepidoptera: Nymphalidae) in Uganda was investigated. Samples of eggs and larvae of the sweetpotato butterfly were collected from some of the major sweetpotato growing districts of Uganda to ...

  7. Evaluation of bioassays for testing Bt sweetpotato events against ...

    African Journals Online (AJOL)

    Sweetpotato weevil (Cylas puncticollis) Boheman is a serious pest throughout Sub-Saharan Africa region and is a big threat to sweetpotato cultivation. Ten transgenic sweetpotato events expressing Cry7Aa1, Cry3Ca1, and ET33-34 proteins from Bacillus thuringiensis (Bt) were evaluated for resistance against C.

  8. Gender differentials in sweetpotato production on the livelihood ...

    African Journals Online (AJOL)

    This study was conducted to analyse the determinants of sweetpotato production on the livelihood strategies of the male and female sweetpotato producers in Ebonyi State. A multi-stage randomized sampling procedure was used to collect cross sectional data in 2014. Data collected from 120 Sweetpotato producers were ...

  9. Uso de diferentes gelificantes e de um esterilizante em cultura de segmentos nodais de batata-doce

    OpenAIRE

    Minamiguchi, Joice Yuri

    2013-01-01

    A cultura da Ipomoea batatas (L.) é de imenso potencial alimentar, tanto para seres humanos quanto para animais. É uma raiz de alto rendimento e rico em nutrientes, que ainda ocupa áreas marginais da agricultura. A propagação é vegetativa utilizando-se ramas ou brotações de raízes para plantio. Porém, estes métodos podem afetar a propagação desta espécie, uma vez que pode disseminar doenças. O objetivo deste trabalho foi avaliar gelificantes e um desinfetante sistêmico na cultura de tecido de...

  10. Taxonomic Evaluation of Fifteen Species of Ipomoea L. (Convolvulaceae from South-Western Nigeria using Foliar Micromorphological Characters

    Directory of Open Access Journals (Sweden)

    Abayomi Ezekiel FOLORUNSO

    2013-05-01

    Full Text Available Foliar micromorphological study was carried out on I. triloba, I. eriocarpa, I. alba, I. hederifolia, I. muricata, I. intrapilosa, I. asarifolia, I. batatas, I. aquatica, I. mauritiana, I. carnea, I.involucrata, I. nil, I. heterotricha and I. obscura to determine the patterns of variation in their epidermal characteristics and search for useful and stable anatomical characters for the identification of the species. Leaf epidermal features that provided useful specific distinctions are cell shape, anticlinal wall pattern, stomata shape, stomata type, trichome, cuticular striations, crystal, granular periclinal wall, stomata size, stomata index, scale and tannin. The characters revealed interrelationships among the Ipomoea species and also suggest their monophyly.

  11. Induction of somatic embryogenesis in recalcitrant sweetpotato ...

    African Journals Online (AJOL)

    fluoroamphetamine (4-FA) and 4,5-trichlorophenoxyacetic acid (2,4,5-T) were investigated in this study for enhancing somatic embryogenesis from various plant organs of recalcitrant African sweetpotato cultivars. 2,4-D was found to be the best (p . 0.05) for ...

  12. Damage reduces shelf-life of sweetpotato during marketing | Mtunda ...

    African Journals Online (AJOL)

    Damage reduces shelf-life of sweetpotato during marketing. ... Although sweetpotato is primarily grown for home consumption, marketing is becoming increasingly important, and in this case, short shelf-life of the roots is a major constraint. An assessment of the levels of ... (African Crop Science Journal 2001 9(1): 301-308).

  13. Potential of orange and yellow fleshed sweetpotato cultivars for ...

    African Journals Online (AJOL)

    The potential of orange and yellow fleshed sweetpotato cultivars as a dietary source of Vitamin A in Mpigi and Luwero Districts of central Uganda was evaluated. On-farm agronomic performance, acceptability and b-carotene content of two orange (SPK004 and 316) and two yellow fleshed (Tanzania and 52) sweetpotato ...

  14. Integrated pest management for sweetpotato in Eastern Africa

    NARCIS (Netherlands)

    Smit, N.

    1997-01-01

    Sweetpotato is an important crop in Eastern Africa. Sweetpotato weevils ( Cylas puncticollis Boheman and C. brunneus Fabricius; Coleoptera: Apionidae) cause damage to roots and vines
    throughout the crop's

  15. Farmers' perceptions of orange-fleshed sweetpotato: Do common ...

    African Journals Online (AJOL)

    Contrary to the common beliefs, the study finds that sweetpotato is an important food crop to producing households, and that the common negative beliefs about sweetpotato production and consumption are not widely held. This study, therefore, recommends the need to upscale and out-scale efforts to sensitize farmers ...

  16. Evaluation of promising sweetpotato genotypes for high altitude ...

    African Journals Online (AJOL)

    Eighteen genotypes from the NationalSweetpotato Programme were evaluated for their fresh storage root yield and reaction to stem and leafbUght (Alternaria bataticola) at Kachwckano (2000 metres above sea level) in Southwest. Uganda. The trials were set up to identify sweetpotato genotypes with adaptation to highland ...

  17. Assessment of the sweetpotato market structure in Nasarawa state ...

    African Journals Online (AJOL)

    Product differentiation existed and there were little or no barriers to entry into the sweetpotato market. The Gini Coefficient values of 0.5457 (wholesalers) and 0.6001 (retailers) were recorded, indicating an existence of some degree of sellers' concentration. This implies that sweetpotato market is an imperfect competitive ...

  18. Evaluation of polycross sweetpotato seedlings for root yield potential ...

    African Journals Online (AJOL)

    The study aimed at determining the root yield potential of the sweetpotato seedlings, the variation in storage root flesh colour and response of the storage roots to major pests and diseases attacking sweetpotato in the field .The experiment was carried out in the screen house and at the Eastern experimental field of National ...

  19. Agronomic performance of new cream to yellow-orange sweetpotato ...

    African Journals Online (AJOL)

    aim of the present study was to assess the stability, agronomic performance and palatability of new ARC cultivars .... Site description for multi-environment trials with sweetpotato cultivars .... Table 4: Combined analysis of variance of AMMI model for yield components in sweetpotato trials across six environments and two.

  20. Influence of sweetpotato rooting characteristics on infestation and ...

    African Journals Online (AJOL)

    Studies were carried out in the field at Serere Agricultural and Animal Production Research Institute (SAARI), Eastern Uganda, to establish whether the existing sweetpotato germplasm in Uganda has cultivars resistant to the sweetpotato weevils, Cylas spp. The trials were conducted during the two growing seasons of 1997.

  1. Field evaluation of yield effects on the U.S.A. heirloom sweet potato cultivars infected by sweet potato leaf curl virus

    Science.gov (United States)

    The incidence of Sweet potato leaf curl virus (SPLCV), a Begomovirus, infection of sweetpotato Ipomoea batatas (L.) Lam. (Convolvulaceae) in South Carolina, USA has increased rapidly in recent years. This is likely due to the use of infected propagating materials and the increasing population of it...

  2. Journal of Agriculture and Food Sciences - Vol 14, No 2 (2016)

    African Journals Online (AJOL)

    Comparative economic assessment of the effect of selected fallow species for soil amendment on the yield of sweetpotato (Ipomoea batatas) alley in the Guinea savannah of Nigeria · EMAIL FULL TEXT EMAIL FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. J.E. Ewuziem, P.O. Anyaegbu, 1-14.

  3. Physicochemical composition and antioxidant properties of a ...

    African Journals Online (AJOL)

    Physicochemical composition and antioxidant properties of a sweetpotato variety ( Ipomoea batatas L) commercially sold in South Eastern Nigeria. ... Results show that this sweet potato variety has potentials of biological properties and could have wide utility in food, alcohol and sugar industries. In addition, it could serve as ...

  4. thidiazuron improves adventitious bud and shoot regeneration in ...

    African Journals Online (AJOL)

    Prof. Adipala Ekwamu

    Induction of adventitious buds and shoots from intact leaves and stem internode segments of two recalcitrant. Ugandan sweetpotato (Ipomoea batatas L.) cultivars was investigated in vitro on Murashige and Skoog (MS) medium, supplemented with 3 different levels (0.5, 2.0 and 4.0 µM) of Thidiazuron (TDZ). Shoots were.

  5. thidiazuron improves adventitious bud and shoot regeneration

    African Journals Online (AJOL)

    Prof. Adipala Ekwamu

    Induction of adventitious buds and shoots from intact leaves and stem internode segments of two recalcitrant. Ugandan sweetpotato (Ipomoea batatas L.) cultivars was investigated in vitro on Murashige and Skoog (MS) medium, supplemented with 3 different levels (0.5, 2.0 and 4.0 µM) of Thidiazuron (TDZ). Shoots were.

  6. utilization of sweetpotato based confection technology

    African Journals Online (AJOL)

    oma

    Keywords: Technology, Sweet potato, utilization, households, southeastern, Nigeria. INTRODUCTION. Sweet potato (lpomoea batatas L) is among the worlds most important, versatile, and under exploited food crops, with more than 133 million tones (FAOSTAT, 1997) in annual production. Sweet potato currently ranks as ...

  7. PENGARUH PENGOLAHAN PANAS TERHADAP KONSENTRASI ANTOSIANIN MONOMERIK UBI JALAR UNGU (Ipomoea batatas L (Efect of Heat Processing on Monomeric Anthocyanin of Purple Sweet Potato (Ipomoea batatas L

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    Ai Mahmudatussa'adah

    2015-09-01

    content in PSP during heat processing of flakes. The other purpose of this reseach was to observe the order kinetics model of effect temperature and time baking on total anthocyanin monomeric of fresh PSP and rehydration PSP flakes. The experimental applied a completely randomized design with three replications. The color and amount of anthocyanin (L * = 23.38 ± 0.71, C = 9.84 ± 0.98, Hue = 12.25 ± 1.61. Total monomeric anthocyanin in fresh PSP was 1.45 ± 0.00 mg cyanidin equivalent (CyE/g dry basis (db. In general, the color and the amount of PSP anthocyanin changed during the flakes processing. Steamed PSP for 7 minutes turned its color into a bright purple (L * = 25.88 ± 0.47, C = 24.64 ± 0.25, Hue = 348.83 ± 0.33 with the amount of monomeric anthocyanin increased to 3.76 ± 0.01 CyE mg/g db. Flakes PSP was very bright purple (L * = 36.12 ± 0.11, C = 9.97 ± 0.18, Hue = 359.29 ± 0.31 and the amount of monomericanthocyanin was slightly lower than that of steamed sweet potato (3.19 ± 0.12 mg CyE / g db. Total monomeric anthocyanin of fresh PSP and rehydration flakes PSP decrease during baking time. Keywords: Color, anthocyanin, purple sweet potato, flakes, degradation kinetics   ABSTRAK Antosianin merupakan salah satu kelompok zat warna alami yang terdapat pada tanaman, seperti daun, bunga, umbi, buah atau sayur. Salah satu sumber antosianin pada tanaman adalah ubi jalar ungu (UJU yang mengandung lebih dari 98% antosianin terasilasi dari konsentrasi antosianin umbi. Warna antosianin bervariasi mulai dari merah, ungu, biru, sampai kuning. Warna dan konsentrasi antosianin dapat berubah karena pengaruh panas. Penelitian ini bertujuan untuk mempelajari perubahan warna dan konsentrasi antosianin monomerik sebagai akibat proses pengolahan dalam pembuatan  UJU. Penelitian ini mengkaji juga mengenai model kinetika reaksi pengaruh suhu dan waktu panggang terhadap konsentrasi antosianin UJU segar dan  rehidrasi. Penelitian menggunakan rancangan acak lengkap dengan tiga kali ulangan. Warna dan konsentrasi antosianin dari UJU segar, setelah proses pengukusandan setelah menjadi  dibandingkan. UJU segar berwarna ungu kecoklatan (L =23,38±0,71, C=9,84±0,98, Hue=12,25±1,61. Konsentrasi antosianin monomerik pada UJU segar adalah 1,45±0,00 mg setara sianidin (CyE/gbasis kering (bk. Secara umum, warna dan konsentrasi antosianin UJU berubah selama proses pembuatan . UJU yang dikukus selama 7 menit berubah menjadi ungu cerah (L* * =25,88±0,47, C=24,64±0,25, Hue=348,83±0,33 dengan konsentrasi antosianin monomerik meningkat menjadi 3,76±0,01 mg CyE/g bk.  UJU berwarna ungu sangat cerah (L =36,12±0,11, C=9,97±0,18, Hue=359,29±0,31 dan konsentrasi antosianin monomerik sedikit lebih rendah dibandingkan ubi jalar setelah dikusus (3,19±0,12 mg CyE/g bk. Jumlah antosianin monomerik UJU segar dan rehidrasi menurun seiring dengan waktu dan suhu pemanggangan. Kata kunci: Warna, antosianin, ubi jalar ungu, kecepatan penururunan

  8. Adoption Potential of Improved Sweetpotato Varieties in Ghana ...

    African Journals Online (AJOL)

    50% yield loss), Grasshoppers (30% yield loss) and Caterpillar (20% yield loss). Pesticides (Actelic 50 EC) was extensively used in pest control. Farmers perceived that, excessive use of pesticides had a negative effect on the sweetpotato yield ...

  9. Seed mucilage from Ipomoea dasysperma.

    Science.gov (United States)

    Singh, V; Pandey, M; Srivastava, A; Srivastava, V

    2007-04-01

    A non-ionic water-soluble galactomannan, having galactose and mannose in 1:6 molar ratio, was isolated from endosperm of the seeds of Ipomoea dasysperma. The seed mucilage was found to have a structure having a linear chain of beta (1-->4) linked mannopyranosyl units with D-galactose side chains attached through alpha (1-->6) linkage to the main chain. I. dasysperma seed gum possesses non-ionic characteristics of commercial seed gums and has potential to be used in food and pharmaceutical industries.

  10. Chemical constituents of Sweetpotato genotypes in relation to textural characteristics of processed French fries

    Science.gov (United States)

    Sweetpotato French fries (SPFF) are growing in popularity but limited information is available on SPFF textural properties in relation to chemical composition. This study aimed to investigate the relationship between chemical components of different sweetpotato varieties and textural characteristics...

  11. Sensitivity of the quarantine pest rough sweetpotato weevil, Blosyrus asellus to postharvest irradiation treatment

    Science.gov (United States)

    Rough sweetpotato weevil, Blosyrus asellus (Olivier), is a new quarantine pest of Hawaii sweetpotatoes. Currently, sweetpotatoes can be exported from Hawaii to the U.S. mainland using a postharvest irradiation treatment of 150 Gy to control three other regulated insect pests. Studies were conducted...

  12. Topdressing fertilization with nitrogen and potassium levels in sweet-potatoAdubação de cobertura na batata-doce com doses combinadas de nitrogênio e potássio

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    José Salvador Simoneti Foloni

    2013-03-01

    Full Text Available Balanced fertilizations with N and K often increase the performance of crops, however, when there is absence of one of these nutrients in poor soils, can reduce crop response to fertilization with the other. The objective of this work was to evaluate the productivity of sweet-potato, the amount of sweetpotato without quality, and leaf nutrients analysis, of sweet-potato crop fertilized with N and K. The trial was conducted in farming for commercial production, in Presidente Prudente, São Paulo State, during February-June 2007, in a dystrophic Ultisol of medium texture. The experimental design was a randomized complete block design, with four replications, on factorial 4 x 4: levels of 0, 30, 60 and 120 kg N ha-1 (urea source combined with levels of 0, 30, 60 and 120 kg K2O ha-1 (KCl source, applied to 39 days after planting of the crop. The sweet-potato is responsive to topdressing application with nitrogen and potassium, however, the greatest increases in productivity occur when doses of N and K are combined. Fertilization with N and K not increase the amount of sweet-potato without quality marketing. The highest increase in productivity of sweet-potato is reached with topdressing combined with 100 kg N ha-1 plus 120 kg K2O ha-1. A adubação balanceada de N e K frequentemente aumenta o desempenho das lavouras, contudo, a falta de um desses nutrientes em solos deficientes pode levar a decréscimos na resposta ao outro. O objetivo deste trabalho foi avaliar a produtividade de batata-doce comercializável, a quantidade de raízes tuberosas para descarte e a diagnose foliar de lavoura de batata-doce adubada com N e K. O experimento foi realizado em lavoura comercial, em Presidente Prudente-SP, de fevereiro a junho de 2007, em um Argissolo Vermelho distroférrico de textura média. O delineamento experimental foi em blocos completos casualizados, com quatro repetições, no esquema fatorial 4 x 4, com 0, 30, 60 e 120 kg ha-1 de N (uréia, em

  13. EVALUACIÓN AGRONÓMICA DE TRECE GENOTIPOS DE CAMOTE (Ipomoea batatas L.

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    Rolbin Castillo Matamoros

    2014-01-01

    Full Text Available El objetivo del presente trabajo fue caracterizar desde el punto de vista agronómico, 13 genotipos de camote para su cultivo en Costa Rica. Diez fueron introducidos de la Unidad de Micropropagación de la Universidad Estatal de Carolina del Norte, y cultivados por primera vez en Costa Rica, 2 conocidos como “Exportación y Zanahoriaˮ son de introducción reciente, pero se han cultivado en el país por al menos 5 años y el restante Criollo es el que ocupa actualmente la mayor área de siembra. El trabajo se llevó a cabo en la Estación Experimental Fabio Baudrit de la Universidad de Costa Rica, ubicada en la provincia de Alajuela. Los tratamientos se dispusieron en el campo en un diseño de bloques completos al azar, con 13 genotipos y 4 repeticiones. El análisis de varianza mostró que los genotipos difirieron significativamente (p=0,0001 en todas las variables evaluadas: peso fresco y seco foliar; peso fresco y seco de raíz reservante; número de raíces reservantes; peso de raíz reservante/ planta; contenido de materia seca y rendimiento (t.ha-1. Todos los cultivares evaluados superaron el rendimiento de la variedad Criollo (6 t.ha-1 y el promedio nacional (5 a 7,8 t.ha-1, con rendimientos entre 12 y 48 t.ha-1. Algunos de los cultivares son promisorios para su cultivo en Costa Rica, no solo con base en su rendimiento, sino debido a su color de pulpa, amarilla o anaranjada, asociado a un mayor contenido de carotenos

  14. Pengaruh Macam dan Waktu Aplikasi Bahan Organik pada Tanaman Ubi Jalar (Ipomoea batatas L. Var. Kawi

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    Nur Edy Suminarti

    2015-07-01

    Full Text Available Diversifikasi pangan merupakan langkah tepat untuk mengantisipasi kondisi rawan pangan.  Hal ini menjadi penting karena setiap tahun luas lahan basah telah mengalami penyusutan sekitar 0,1% dari total luas lahan di Indonesia. Sehubungan dengan hal tersebut, dan dalam upaya untuk memenuhi kebutuhan pangan nasional, maka pemanfaatan ubi jalar sebagai sumber bahan pangan alternatif perlu dilakukan. Penelitian yang bertujuan untuk mendapatkan informasi tentang macam dan waktu aplikasi bahan organik yang tepat telah dilakukan di Desa Landungsari, Kabupaten Malang. Rancangan yang digunakan adalah Petak Terpisah, macam bahan organik ditempatkan pada petak utama, terdiri dari 3 macam, yaitu  pupuk kandang sapi, kompos azolla dan kompos sampah kota.  Waktu aplikasi bahan organik sebagai anak petak, terdiri dari 3 macam, yaitu : 30 hari sebelum tanam, 15 hari sebelum tanam dan bersamaan tanam. Pengumpulan data dilakukan secara destruktif, meliputi  komponen pertumbuhan dan panen, analisis pertumbuhan tanaman dan analisis tanah. Uji F taraf 5% ditujukan untuk menguji pengaruh perlakuan, sedang perbedaan diantara rata-rata perlakuan didasarkan pada nilai BNT taraf 5%. Interaksi nyata terjadi pada sebagian besar parameter yang diamati, dan hasil umbi tertinggi didapatkan pada kompos sampah kota yang waktu aplikasinya dilakukan 30 hari sebelum tanam : 28,03 ton umbi ha-1. Food diversification is a precise step to anticipate food shortage condition. It becomes important because wetland area is decreasing of approximately 0,1% each year of total area in Indonesia. Related to that point, also in order to meet food national demand, utilization of sweet potato as alternative food resource needs to be done. Research aimed to get information about proper kind and application time of organic matter had been done at Landungsari, Malang. The design used was Split Plot, kind of organic matter was placed as main plot, consisting of 3 kinds, ie: cow manure, azzola compost, and urban waste compost. Application time was set as subplot, consisting of 3 times, ie: 30 days before planting, 15 days before planting, and simultaneous with planting. Data collection was carried out destructively, including growth and yield components, plant growth analysis and soil analysis. F test on 5% level intended to test treatment effect, meanwhile difference between mean values based on LSD 5%. Real interaction occurred on almost all parameters observed, moreover the highest tuber yield was on urban waste compost which time application was 30 days before planting : 28,03 ton tuber ha-1.

  15. The Uptake of 134Cs From Soil To Sweet Potatoes Crops (Ipomoea batatas)

    International Nuclear Information System (INIS)

    Octolia Togibasa T; Idam Arif; Putu Sukmabuana, Poppy Intan Tjahaja

    2009-01-01

    This research studied the uptake of radiocesium from soil to crops. Study on food crops required to know the dose that can be received by human if there is a reactor accident or as a result of the global fall out in the area of tropical climate. Sweet potato crops cultivated on soil was contaminated with 134 Cs with the concentration of 167.62 Bq/gr. Sweet potatoes also cultivated on soil non contaminated as a control. Observation was carried out every 7 days for the contaminated media and 14 days for the control media, by sampling 3 plants and soil. The plants and soil samples were dried in with a temperature of 100 o C for 3 hours in the oven, and then counted using gamma spectrometer. The results indicated that sweet potato crops have significant ability to uptake 134 Cs from soil. The maximum plant uptake took place at the beginning of the cultivation with T F value of 1.26 and distributed to the entire plant.The tuber was formed at the sixth week, with T F value of 13.16. The highest concentration located at the root and tuber, it is important to note because the tuber is the main part of sweet potatoes crops which consumed by human. (author)

  16. The effect of gamma radiation on the ultrastructure of sweet potatoes (Ipomoea batatas)

    Energy Technology Data Exchange (ETDEWEB)

    Brown, A.

    1986-12-01

    Radiation is being used to increase the storage life of fresh foods. Various doses of gamma radiation were administered to Jewel cultivar sweet potatoes and the effects were monitored by direct observation and by scanning and transmission electron microscopy. Potatoes were divided into two groups: those irradiated immediately after harvest (doses = 0 kGy - 0.4 KGy) and those irradiated one week after harvest (doses = 0 kGy - 0.4 kGy). Potatoes were examined and viewed each month for 7 months. Gross observations included weight, color and texture of the sweet potatoes. Those potatoes irradiated immediately after harvest spoiled faster than those irradiated one week after harvest. Scanning electron microscopy demonstrated several cellular modifications accompanying spoilage. Cell collapse was greatest at the higher radiation doses during the periods of 1 to 5 months post-irradiation. The shape and size of starch granules varied with storage time and radiation levels. The mitochondria, cell walls and plasma membranes appeared normal as seen by transmission electron microscopy until 6 months post-irradiation for potatoes irradiated both immediately after harvest and one week after harvest. Thereafter, degradative changes were observed.

  17. Preparative purification of polyphenols from sweet potato (Ipomoea batatas L.) leaves by AB-8 macroporous resins.

    Science.gov (United States)

    Xi, Lisha; Mu, Taihua; Sun, Hongnan

    2015-04-01

    In this study, the adsorption properties of AB-8 macroporous resin for sweet potato leaf polyphenols was investigated. The adsorption mechanism was elaborated by the Langmuir and Freundlich equations, and the purification parameters were optimised by adsorption and desorption tests. The constituents and their contents of the purified products were analysed, and the antioxidant activities were determined. The results showed that the optimal processing parameters were as follows: an initial polyphenol concentration of 2.0mg chlorogenic acid equivalent (CAE)/ml, pH 3.0, an ethanol desorption solution concentration of 70% (v/v) and a flow rate for feeding and elution of 1BV/h. The purified products mainly contained eight phenolic constituents and the contents of three di-caffeoylquinic acids were relatively higher than the other constituents. The purified products possessed strong antioxidant activities. In conclusion, purification by AB-8 macroporous resin was highly efficient, economic and environmentally friendly and has a great industrial production potential. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. [Stress-responsive expression analysis of glutathione-S-transferase gene of Ipomoea batatas (L.) Lam].

    Science.gov (United States)

    Liu, Xun; He, Bo-Wen; Zhang, Yi-Zheng

    2009-08-01

    A prokaryotic expression plasmid pET-IbGST, which contains the full encoding region of a glutathione-S- transferase (GST) gene of sweet potato, was constructed. The recombinant IBGSTU1 protein was expressed in Escherichia coli and found in the soluble fraction, as well as in insoluble inclusion bodies of lysed cells. Its enzymatic activity was detected using UV spectrophotometer. The protein was purified and used to prepare antibody. Semi-quantitative RT-PCR and Western blotting analyses demonstrated that IBGSTU1 gene was not expressed under normal conditions. When subjected to some environ-mental stresses such as cold-stress or heavy-medal stress, the organism switches on the expression of IBGSTU1 at both mRNA and protein levels, and its expression has tissue specificity.

  19. Mercury content of sprouts and harvested roots from treated sweet potato mother roots. [Ipomoea batatas

    Energy Technology Data Exchange (ETDEWEB)

    Huisingh, D.; Nielsen, L.W.

    1972-01-01

    Mercury containing fungicides have been used extensively for seed and root disease control, but data on the fate of the mercury (Hg) are scarce. Experiments were designed to see if Hg applied to propagative sweet potato roots increased the Hg-content of edible roots. Roots were treated with Semesan Bel(hydroxymercurinitrophenol + hydroxymercurichlorophenol), Mertect (Thiabendazole: 2-(4-Thiazolyl)-benzimidazole), or Botran (2,6-Dichloro-4-nitroanaline) at recommended rates or with water. Treated roots were bedded into sandy loam soil, and the plants were harvested at 2 and 3 months after bedding. Some sprouts transplanted at 2 months were grown to maturity, and the harvested roots were analyzed. Hg analyses were performed by flameless atomic absorption. Roots treated prior to planting with Semesan Bel, Mertect, Botran, and water contained 23.0, 0.05, 0.03 and 0.03 ..mu..g/g dry wt, respectively. At the 2-month harvest, the leaves and stems of the Semesan Bel-treated plants contained 5 times more Hg than those of the other treatments. By the 3-month harvest, the amount of Hg in plant leaves and stems from Hg-treated roots was 2 to 3 times that of the others. Fall harvested fleshy roots contained 0.03, 0.02, 0.03, and 0.03 ..mu..g/g dry wt Hg for the Semesan Bel, Mertect, Botran, and water treatments, respectively. This demonstrates that the Hg applied to the mother root was translocated to the new plant, but little if any was translocated to the new fleshy roots.

  20. Evaluation of metals in several varieties of sweet potatoes (Ipomoea batatas L.): comparative study.

    Science.gov (United States)

    Luis, Gara; Rubio, Carmen; Gutiérrez, Angel J; González-Weller, Dailos; Revert, Consuelo; Hardisson, Arturo

    2014-01-01

    Sweet potatoes are a staple in the diet of some people and an excellent source of minerals. Metal monitoring in food, like sweet potatoes, provides basic information on safety aspects in regulatory processes as well as nutritional values. One hundred five samples of three varieties of sweet potatoes were randomly obtained from supermarkets, farmers markets, and farmers' plots in Tenerife (Canary Islands, Spain). The edible portion (pulp) was the only part considered for analysis. Flame atomic absorption spectrometry was used to determine the contents of sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn), while the levels of chromium (Cr), nickel (Ni), cadmium (Cd), and lead (Pb) were determined using graphite furnace atomic absorption spectrometry. The mean concentrations were 500 Na, 4409 K, 564 Ca, 609 Mg, 1.291 Cu, 6.554 Fe, 2.324 Mn, 2.348 Zn, 0.028 Cr, 0.048 Ni, 0.001 Cd, and 0.003 Pb mg/kg, respectively. Potassium presented the highest contents in all varieties of sweet potatoes. Iron was the most abundant microelement. The orange fleshed sweet potato variety offered greater nutritional contributions to the recommended intakes than the rest of the varieties studied. The estimated mean daily intake of Ni (0.72 mg/day) detected in our samples was highly consistent with other studies. Average daily intakes of Cd (0.015 μg/day) and Pb (0.045 μg/day) were below toxicological reference values. In conclusion, the levels of Cd and Pb detected in the sweet potatoes analyzed do not represent any toxicological risk to consumers.

  1. in vitro production of virus free sweet potato [ipomoea batatas (l

    African Journals Online (AJOL)

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    facilitation, most frequently described from figs. (Harrison et al., 2003). In forests and outside forest condition, figs take advantage of a host to escape herbivory and reach the upper canopy much sooner than seedlings established on the ground (Werner and Gradstein, 2008). Schefflera abyssinica (Araliaceae) is dominant.

  2. in vitro production of virus free sweet potato [ipomoea batatas (l

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    the least (2.63 mg/kg) was for enjera processed from brown tef grain baked using tap water. Daily dietary fluoride intake was different with ... studies, due to the increased health benefits associated with high iron contents in brown tef ... drinking water or food results in dental fluorosis and skeletal fluorosis (Liub et al., 2009).

  3. in vitro production of virus free sweet potato [ipomoea batatas (l

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    enset is reported from Vietnam, where it provided an emergency food during the Second. World War. In parts of north and central. Vietnam, the growing point was used as a vegetable (Admasu Tsegaye and Westphal, 2002). ... which show great variation in culture and agricultural practices (Admasu Tsegaye and. Westphal ...

  4. in vitro production of virus free sweet potato [ipomoea batatas (l

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    ABSTRACT: Pure cellulose was used as sole carbon source for the production of cellulase by. Trichoderma isolates under liquid state .... (peptone, ammonium sulphate, sodium nitrate and yeast extract). The control was prepared in ... To determine the effect of metal ions; salt solutions were used for source of the metal ions.

  5. Physiological responses of sweet potato (Ipomoea batatas L. plants due to different copper concentrations

    Directory of Open Access Journals (Sweden)

    Cristina Copstein Cuchiara

    2015-12-01

    Full Text Available At low concentrations, Cu is considered as an essential micronutrient for plants and as a constituent and activator of several enzymes. However, when in excess, Cu can negatively affect plant growth and metabolism. Therefore, the aim of this study was to evaluate physiological responses of sweet potato plants at different Cu concentrations by measuring morphological parameters, antioxidant metabolism, stomatal characteristics, and mineral profile. For this purpose, sweet potato plants were grown hydroponically in complete nutrient solution for six days. Then, the plants were transferred to solutions containing different Cu concentrations, 0.041 (control, 0.082, and 0.164 mM, and maintained for nine days. The main effect of increased Cu concentration was observed in the roots. The sweet potato plants grown in 0.082 mM Cu solution showed increased activity of antioxidant enzymes and no changes in growth parameters. However, at a concentration of 0.164 mM, Cu was transported from the roots to the shoots. This concentration altered morpho-anatomical characteristics and activated the antioxidant system because of the stress generated by excess Cu. On the basis of the results, it can be concluded that the sweet potato plants were able to tolerate Cu toxicity until 0.082 mM.

  6. Effects of incorporating nonmodified sweet potato (Ipomoea batatas) flour on wheat pasta functional characteristics.

    Science.gov (United States)

    Saleh, Mohammed; Lee, Youngseung; Obeidat, Hayat

    2017-12-28

    The effects of substituting wheat flour using fractions of blanched or nonblanched sweet potato flour on produced pasta functional characteristics were investigated. The use of sweet potato flour to replace fractions of wheat flour, regardless of blanching treatment, resulted in significant (p sweet potato flour and held at 55C resulted in 27% point increase in WHC compared to the control. Sweet potato flour was also contributed to the decrease in treatments pasting viscosities and in cooked pasta hardness. Cooked pasta hardness was significantly (p sweet potato flour replacements, respectively. Flow behavior index of wheat flour treatments containing fractions of sweet potato flour were fitted in a shear thinning model. Quality indices of pasta made using fractions of sweet potato were equivalent to or sometimes superior to that of the control sample. Results indicated the possible enhancement of pasta nutritional quality including firmness, cooking loss, and water uptake without impairing consumer acceptability. The use of sweet potato flour in pasta would enhance the nutritional and physicochemical properties of developed pasta and pasta products. Furthermore, since sweet potato is not cultivated most of the year; sweet potato flour pasta would be better utilized in food processing and is expected to enhance sweet potato consumption year around. © 2017 Wiley Periodicals, Inc.

  7. Sweet potato (Ipomoea batatas) attenuates diet-induced aortic stiffening independent of changes in body composition.

    Science.gov (United States)

    Garner, Tyler; Ouyang, An; Berrones, Adam J; Campbell, Marilyn S; Du, Bing; Fleenor, Bradley S

    2017-08-01

    We hypothesized a sweet potato intervention would prevent high-fat (HF) diet-induced aortic stiffness, which would be associated with decreased arterial oxidative stress and increased mitochondrial uncoupling. Young (8-week old) C57BL/6J mice were randomly divided into 4 groups: low fat (LF; 10% fat), HF (60% fat), low-fat sweet potato (LFSP; 10% fat containing 260.3 μg/kcal sweet potato), or high-fat sweet potato diet (HFSP; 60% fat containing 260.3 μg/kcal sweet potato) for 16 weeks. Compared with LF and LFSP, HF- and HFSP-fed mice had increased body mass and percent fat mass with lower percent lean mass (all, P Sweet potato intervention did not influence body composition (all, P > 0.05). Arterial stiffness, assessed by aortic pulse wave velocity and ex vivo mechanical testing of the elastin region elastic modulus (EEM) was greater in HF compared with LF and HFSP animals (all, P sweet potato attenuates diet-induced aortic stiffness independent of body mass and composition, which is associated with a normalization of arterial oxidative stress possibly due to mitochondrial uncoupling.

  8. The effect of gamma radiation on the ultrastructure of sweet potatoes (Ipomoea batatas)

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    Brown, A.

    1986-12-01

    Radiation is being used to increase the storage life of fresh foods. Various doses of gamma radiation were administered to Jewel cultivar sweet potatoes and the effects were monitored by direct observation and by scanning and transmission electron microscopy. Potatoes were divided into two groups: those irradiated immediately after harvest (doses = 0 kGy - 0.4 KGy) and those irradiated one week after harvest (doses = 0 kGy - 0.4 kGy). Potatoes were examined and viewed each month for 7 months. Gross observations included weight, color and texture of the sweet potatoes. Those potatoes irradiated immediately after harvest spoiled faster than those irradiated one week after harvest. Scanning electron microscopy demonstrated several cellular modifications accompanying spoilage. Cell collapse was greatest at the higher radiation doses during the periods of 1 to 5 months post-irradiation. The shape and size of starch granules varied with storage time and radiation levels. The mitochondria, cell walls and plasma membranes appeared normal as seen by transmission electron microscopy until 6 months post-irradiation for potatoes irradiated both immediately after harvest and one week after harvest. Thereafter, degradative changes were observed

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    The incidents of landslides and other types of mass movements have accounted not only for the loss of life of people and animals but have also damaged or ... Several techniques have been developed worldwide to demarcate and investigate landslide hazard zones. One such technique is expert evaluation approach, ...

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    It was previously reported that the African green monkey (grivet monkey) could serve as a non–human primate model of L. aethiopica infection. This report provides preliminary data on the development and progression of skin lesions in grivet monkeys when infected with L. aethiopica either in the eye lids or the tip of nose.

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    ABSTRACT: The current research work was designed to evaluate, test, and characterize effective antifungal extracts from Trichoderma isolates against coffee wilt pathogen (Gibberella xylarioides). For extraction of antifungal extracts from fungal mycelium different organic solvents, viz., chloroform, ethanol, methanol, ethyl ...

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    PO Box 31167, Addis Ababa, Ethiopia.E-mail: mengistu.goa@aau.edu.et. 3 Department of Mathematics, College of Sciences, Botswana International University of Science and. Technology, Private Mail Bag 16, Palapye, Botswana. E-mail: habtuzh@yahoo.com. ABSTRACT: In this paper, we introduce and investigate an ...

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    ABSTRACT: Viral disease is the major factor causing significant yield loss in sweet potato. Production of disease-free clones by tissue culture technique increases yield and income of farmers. Meristems from three varieties of sweet potato were cultured at different combinations of BAP, GA3 and NAA in MS basal medium.

  14. Mycoflora of sun-dried sweet potato (Ipomoea batatas L.) slices in ...

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    2009-07-20

    Jul 20, 2009 ... A study was carried out to isolate and quantify the fungi present in sun-dried sweet potato slices in. Benin City, Nigeria. Potato tubers were peeled, washed, sliced and sun-dried for 30 days. Oven-dried slices served as control. Meteorological data were obtained for the period of study. Fungal colonies on.

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    transgenic resistance” and “engineering resistance”, has attracted major interest and is the main one ..... Biotechnology Symposium. October 17–22,. 2004, Santiago, Chile. 24. Kobayashi, K., Cabral, S., Calamante, G.,. Maldonado, S. and Mentaberry, ...

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    2008). Habitat isolation changes the beta diversity of the vascular epiphyte community in lower montane forest, Veracruz, Mexico. Biodiversity and Conservation 17:191–207. 9. Fichtl, R., and Admasu Adi (1994). Honey Bee Flora of Ethiopia.

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    wheat hybrids were selected by EIAR and these were evaluated over a period of two years from. 2007 to 2008 in four locations namely, Adet,. Holeta, Kulumsa and Sinana under irrigated con- dition. The experimental layout was a rando- mized complete block design (RCBD) with four replications. Planting method was on ...

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    of energy and nutrient needs. Staple foods vary by location but typically include grains, tubers, legumes or seeds (World Bank, 2012). Grains such as tef .... boreholes and 2 to 68 mg/L in geothermal wells. (Tamiru Alemayehu, 2006). The Wonji area and its surroundings contain high fluoride in the groundwater attributed to ...

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    ABSTRACT: The phylogeny of the East African Hippopotamidae is problematic. A particularly controversial relationship is that between aff. Hippopotamus protamphibius and aff. .... ficient reconstruction of the symphysis, corpora, and canine processes has been completed to warrant a preliminary description of the fossil.

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    E-mail: Henok.Tiruneh@mines.sdsmt.edu. 2 School of Earth Sciences, College of Natural Sciences, PO Box 1176, Addis Ababa University, Addis ... Landslides are significant natural hazards in mountainous terrain all around the world. ...... for the purpose of agriculture and dwelling. Figure 9. Landslide hazard zonation of ...

  1. 61-64 Registration of "Tola" Sweet Potato [Ipomoea batatas(L.

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    Potato Post Harvest in East Africa,” CIP Sub-. Project Annual Progress Report, CIP Library,. Lima, pp 24 - 50. Purseglove, J. A. 1972. Tropical crops: Dicotyledons. Landon, Longman. Teshome Anshebo. 2002. Evaluation of sweet potato. (Ipomeabatatas Lam.) clones for high yield storage root yield with high starch and low ...

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    ABSTRACT: Wild edible plants are valuable resources in rural livelihoods for supplementing the staple food, ensuring food security, dietary diversification and sustained income. This study aimed to identify and document indigenous uses and management of wild edible plants being used by the. Afar and Oromo ...

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    nitrogen source, cellulose concentration, cultivation temperature and pH on enzyme production were optimized using LSF. ... cellulase producing Trichoderma isolates can be used in food industries, animal feed industries, brewing and wine making, ..... Production with the. Basal Medium Palm Oil Mill Effluent.Academy.

  4. In vitro Production of Virus-Free Sweet Potato [ Ipomoea batatas (L ...

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    There was 100% sweet potato virus elimination from all the three varieties by meristem culture as observed by using NCM-ELISA technique. Shoot thermotherapy was done for Awassa-83 and Awassa local at 37°C for 31 days and 88.89% and 100% SPFMV and SPCSV virus elimination was achieved for the two varieties, ...

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    Throughout this section unless otherwise stated, denotes a nonempty, closed and convex subset of a real Hilbert space . For every point ∈ , there exists a unique nearest point in , denoted by. , such that ‖ − ‖ = inf{‖ − ‖ ∶ ∈. }. is called the metric projection of onto . The following characterizes the metric projection.

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    1 Department of Biology, Adigrat University, PO Box 50, Adigrat, Ethiopia. E-mail: mebrahtuhishe@gmail.com. 2 Department of Biology, Bahir Dar University, PO Box 79, Bahir Dar Ethiopia. E-mail: berhanu.tsegay@yahoo.com. ABSTRACT: ..... increment of mistletoes. Sometimes non-host species could be infected under ...

  7. EVALUATION OF ELASTICITY AND MECHANICAL PROPERTIES OF BREAD DOUGH MADE WITH REPLACED FLOUR POTATO (IPOMOEA BATATA

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    Ely Fernando Sacón Vera

    2016-10-01

    Full Text Available The effect of the incorporation of sweet potato flour, with 30% replacement in 1kg of wheat flour was evaluated to determine the behavior of elastic and mechanical properties during the kneading and baking stage of bread. For the evaluation the following varieties were studied: Morado Brazil, Morado Ecuador, Guayaco Morado, Ina and Toquecita, and the evaluated properties were: texture (hardness, elasticity, firmness, chewiness measured by a texture meter Bloomfield and volume was measured by INEN standard (NTE INEN 0530: 80. The design employed was completely at random, using analysis of variance at 5% significance level. The results obtained showed that elasticity attribute in texture variable presented significant differences (P <0.05. Analysis concluded that including Toquecita flour in the mixture to form the dough, had the highest elasticity of 13.32mm. However, Morado Ecuador variety flour presented a 6.24 mm elasticity value, ideal for both the malleability of the dough and the freshness of the bread, and concerning volume, the inclusion of Ecuador Morado flour and Ina in the formulation of bread, showed an increase in volume at 93.30 and 93.67cm3 respectively, close to the normed value for wheat flour bread.

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    Computers and. Electronics in Agriculture 75:347–354. 41. Yuan, F., Sawaya, K.E., Loeffelholz, B.C. and. Bauer, M.E. (2005). Land cover classification and change analysis of the Twin Cities. (Minnesota) metropolitan area by multi- temporal Landsat remote sensing. Journal of. Remote Sensing of Environment 98:317–328.

  9. CARACTERIZACIÓN AGRONÓMICA DE GERMOPLASMA DE CAMOTE (Ipomoea batatas L. EN MANABÍ

    Directory of Open Access Journals (Sweden)

    Carmen Mariuxi Macías Figueroa

    2011-06-01

    Full Text Available La investigación tuvo como objetivo identificar genotipos de camote de alto rendimientos en raíces frescas y follaje. Se evaluaron 4 materiales locales y 11 introducidos del Centro Internacional de la Papa, CIP-Perú. El manejo del cultivo estuvo acorde a las recomendaciones del Programa Yuca-Camote del INIAP. Se evaluaron variables del follaje y raíces. Para el follaje fueron: numero de guías, peso a los 120 y 140 días después de la siembra y en la cosecha. En las raíces se evaluaron: numero de raíces y peso tanto en las comerciales y no comerciales, y rendimiento. Además, se identifico las principales plagas y benéficos que se presentaron en el cultivo. Los resultados de la caracterización agronómica permitieron observar gran variabilidad genética y del potencial productivo. Zapallo, Philipino y Morado-Ecuador mostraron un comportamiento de doble propósito al tener los mejores rendimientos en follaje y raíces a diferencia de Toquecita que se destaco solo en producción de raíces. Se identificaron artrópodos plagas y benéficos. Las principales especies de plagas estuvieron los de los generos : Omophoeta sp, Empoasca sp, Atta sp, Frankliniella sp, Dysdercus sp, Hedylepta sp, Aphis sp y Tetranychus sp. De los benéficos se identificaron cuatro a nivel de género y cinco a nivel de familia. La caracterización agronómica demostró la amplia variabilidad genética de los materiales en la zona en estudio identificándose cuatro materiales con potencial productivo.

  10. Evaluación agronómica de trece genotipos de camote (Ipomoea batatas L

    Directory of Open Access Journals (Sweden)

    Rolbin Castillo Matamoros

    2014-12-01

    Full Text Available El objetivo del presente trabajo fue caracterizar desde el punto de vista agronómico, 13 genotipos de camote para su cultivo en Costa Rica. Diez fueron introducidos de la Unidad de Micropropagación de la Universidad Estatal de Carolina del Norte, y cultivados por primera vez en Costa Rica, 2 conocidos como “Exportación y Zanahoriaˮ son de introducción reciente, pero se han cultivado en el país por al menos 5 años y el restante Criollo es el que ocupa actualmente la mayor área de siembra. El trabajo se llevó a cabo en la Estación Experimental Fabio Baudrit de la Universidad de Costa Rica, ubicada en la provincia de Alajuela. Los tratamientos se dispusieron en el campo en un diseño de bloques completos al azar, con 13 genotipos y 4 repeticiones. El análisis de varianza mostró que los genotipos difirieron significativamente (p=0,0001 en todas las variables evaluadas: peso fresco y seco foliar; peso fresco y seco de raíz reservante; número de raíces reservantes; peso de raíz reservante/ planta; contenido de materia seca y rendimiento (t.ha -1 . Todos los cultivares evaluados superaron el rendimiento de la variedad Criollo (6 t.ha -1 y el promedio nacional (5 a 7,8 t.ha -1 , con rendimientos entre 12 y 48 t.ha -1 . Algunos de los cultivares son promisorios para su cultivo en Costa Rica, no solo con base en su rendimiento, sino debido a su color de pulpa, amarilla o anaranjada, asociado a un mayor contenido de carotenos.

  11. ALTERNATIVAS DE SIEMBRA DEL CAMOTE (Ipomoea batatas L. PARA EL CANTON JARAMIIJO, PROVINCIA DE MANABI

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    Nelson Enrique Motato Alarcón

    2016-06-01

    Full Text Available El trabajo se realizó entre agosto/2014 y enero /2015, en el cantón Jaramijó, provincia de Manabí, tuvo el propósito de generar información tecnológica relacionada con variedades (2, distancias entre plantas 3 y longitudes de guía (3, en el cultivo de camote; complementariamente se hizo un análisis económico de los 18 tratamientos, distribuidos en tres repeticiones, en un diseño de Bloques Completos al Azar bajo un Arreglo Factorial 2x3x3. El análisis estadístico de los datos: números de guía por planta, producción de follaje y número de camotes y rendimiento de raíces frescas por hectárea que permitieron determinar que la variedad Toquecita fue más productiva que Guayaco Morado, la mejor combinación fue la variedad Toquecita sembrada a 0,50 m o 0,30 m de distancia entre plantas y con guías de 0,50 m de tamaño. Económicamente, la alternativa con fines de comercialización externa y/o producción industrial de alimentos recomendada es la de utilizar Toquecita sembrada a 0,40 m o 0,50 m entre plantas y con guías de 0,30 m o 0,50 m de longitud; otra opción que considera la preferencia de los consumidores en el mercado nacional, es la de emplear Guayaco Morado, sembrado a 0,50 m entre plantas y con guías de 0,40 m o 0,50 m de longitud.

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    cyanobacteria in Lake Kuriftu. Key words/phrases: Grazing, Lake Kuriftu, phytoplankton, size .... Driver (1963). A sample of 200 to 500 ml was filtered on a. GF/C filter for chlorophyll-a analysis from each sample (initial and manipulated). The pigments were extracted with alkaline (basic) methanol. Chlorophyll-a (μg/l) =.

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    -driven re- settlements from eastern part of the country on the banks of the Didessa River drove buffaloes and co-existing wildlife further east to the only surviving CTW. The Didessa River catchment constitutes the upper Blue Nile catchment.

  14. Evaluación de ocho nuevos clones de boniato (Ipomoea batatas (L. Lam.

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    Dania Rodríguez del Sol

    2015-09-01

    Full Text Available In Cuba results necessary identifying new sweet potato variety that it‟s possess bigger precociousness, that is, high yield in cycles inferior to five months. The present work was done at Institute of Tropical Root (INIVIT. It involved to evaluate six new sweet potato variety: „INIVIT B 18‟, „INIVIT B 12-9‟, „INIVIT B 13-9‟, „INIVIT B-14‟, „INIVIT B 240-2010‟ e „INIVIT B 16-2010‟ and the commercial variety „CEMSA 78 -354‟ e „INIVIT B2-2005‟, the ones that were considered like control. The clone INIVIT B 240-2010 presented values of 663.5 g and 550.0 g of weight of marketable tuberous roots for winter epoch and spring respectively and it was significantly superior to the rest of the variety in both epochs. The weight for marketable tuberous root, independently of the variety, it‟s different between both epochs of plantation with significant superiority for the epoch of winter as compared with spring epoch. The variety INIVIT B2-2005 presented bigger number of marketable tuberous roots by plant with significant differences to the rest of variety in both epochs. Variety INIVIT B 240-2010 and INIVIT B 16-2010 showed the bigger yield with values of 51.3 t.ha 1 and 49.0 t.ha 1 respectively, significantly superior to the rest of variety.

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    Arundinaria alpina K. Schum. Poaceae. Wosha. Kerkeha. Arundo donax L. Gramineae. Shemboko. Shembeko. Bersama abyssinica Fresen. Melianthaceae. Bo`a. Azamir. Brachystelma lineare A. Rich. Asclepiadaceae. Alwo. Brucea antidysenterica J.F.Mill. Simaroubaceae. Tolo. Buddleja polystachya Fresen. Loganiaceae.

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    G3pdh is under diversifying selection in both populations. This may indicate the importance for isoamylase1 in starch quality traits in cassava, a trait that is likely to have been the target for artificial selection by farmers and breeders, in addition to natural selection. This study also suggests that G3pdh may be a good marker ...

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    intervals. Zooplankton samples were collected using a haul net (mesh size of 67 µm with 31 cm diameter) from 2 m (littoral site) and 6 m depth. (open water). Phytoplankton samples were collected ... litter sample) was taken for zooplankton count- ing using a ... where N is number of cells, A is area of field. (mm2), Depth of a ...

  18. Evaluation of the effects of sweet potato (Ipomoea batatas (L.) Lam) in broiler diets.

    Science.gov (United States)

    Pandi, J; Glatz, P; Forder, R; Komolong, B; Chousalkar, K

    2018-02-01

    Cereal grains such as maize and wheat are used extensively in feed formulations for poultry as the primary source of carbohydrates. High cost of these grains in many developing countries necessitates the evaluation of other ingredients that are grown locally. Sweet potato is one such crop. The study was conducted as a proof of concept experiment to test the hypothesis that in the presence and absence of enzyme, sweet potato roots when included in diets of broiler chickens may affect the total metabolisable energy content of the diets which may exert certain influences on dry matter digestibility of these diets as well as impacting on production and certain gut parameters. A total of 120 chicks were raised on a commercial starter feed from day 0 to 19. On day 22, the birds were individually weighed and allocated to 96 single bird metabolism cages to conduct a 7-day classical apparent metabolisable energy (AME) assay. The test diets contained 0% and 25% sweet potato flour (SPF) with and without enzyme supplementation (Rovabio Excel AP T-flex) and replicated 24 times. AME of the control diet with and without enzyme was 14.05 and 13.91 MJ/kg whilst the AME of the SPF diets with and without enzymes were 13.45 and 13.43 MJ/kg respectively. AME of SPF was 12.08 MJ/kg. Birds fed the SPF had significantly reduced end weights (p = .002) and weight gains (p < .001) leading to significantly higher intake (p = .004) and FCRs (p < .001) in birds. These effects in growth parameters highlight the need to balance dietary protein and total amino acids when using SPF in broiler diets and may not be a negative effect of SPF per say as AME and dry matter digestibility of SPF diets were comparable to the control diet. The level of sucrase activity in the jejunum was significantly (p < .001) lower due to enzyme inclusion. Use of SPF in the current study did not negatively influence the activities of the brush border enzymes maltase and sucrase, gut morphology in the jejunum of broilers or the load of Enterobacteriaceae in the caecal of birds. This finding is promising in that the gut parameters associated with digestive capacity and gut health were not compromised with feeding of SPF to broilers. © 2017 Blackwell Verlag GmbH.

  19. Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene from Ipomoea batatas (L.) Lam.

    Science.gov (United States)

    Yong, Bin; Wang, Xiaoyan; Xu, Pan; Zheng, Haiyan; Fei, Xueting; Hong, Zixi; Ma, Qinqin; Miao, Yuzhi; Yuan, Xianghua; Jiang, Yusong; Shao, Huanhuan

    2017-01-01

    As an indicator of the antioxidant capability of plants, catalase can detoxify reactive oxygen species (ROS) generated by environmental stresses. Sweet potato is one of the top six most important crops in the world. However, its catalases remain largely unknown. In this study, a catalase encoding gene, IbCAT2 (accession number: KY615708), was identified and cloned from sweet potato cv. Xushu 18. It contained a 1479 nucleotides' open reading frame (ORF). S-R-L, Q-K-L, and a putative calmodulin binding domain were located at the C-terminus of IbCAT2, which suggests that IbCAT2 could be a peroxisomal catalase. Next-generation sequencing (NGS) based quantitative analyses showed that IbCAT2 was mainly expressed in young leaves and expanding tuberous roots under normal conditions. When exposed to 10% PEG6000 or 200 mmol/L NaCl solutions, IbCAT2 was upregulated rapidly in the first 11 days and then downregulated, although different tissues showed different degree of change. Overexpression of IbCAT2 conferred salt and drought tolerance in Escherichia coli and Saccharomyces cerevisiae . The positive response of IbCAT2 to abiotic stresses suggested that IbCAT2 might play an important role in stress responses.

  20. in vitro production of virus free sweet potato [ipomoea batatas (l

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    , potato virus Y, tobacco (Nicotiana benthamiana). INTRODUCTION. Protection of plants from viral diseases has often been a difficult task unless there exists a source of natural resistant gene within a species to be used for gene introgression.

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    the appearance of fish thus resulting in consumer rejection. Several groups of .... Data were captured into electronic data sheet. Records of the lengths ..... Clinostomum com- planatum infection in posterior wall of pharynx of a human. Japanese Journal of. Parasitology 36:142–144. 17. Kassahun Asmare (2005). Distribution ...

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    environment trials (MET) to test their per- formance across environments and to select the ... Analysis of variance and estimation of variance components. The relative performance of genotypes based on the mean grain yield (kg ha-1) environments.

  3. Scanning of transposable elements and analyzing expression of transposase genes of sweet potato [Ipomoea batatas].

    Science.gov (United States)

    Yan, Lang; Gu, Ying-Hong; Tao, Xiang; Lai, Xian-Jun; Zhang, Yi-Zheng; Tan, Xue-Mei; Wang, Haiyan

    2014-01-01

    Transposable elements (TEs) are the most abundant genomic components in eukaryotes and affect the genome by their replications and movements to generate genetic plasticity. Sweet potato performs asexual reproduction generally and the TEs may be an important genetic factor for genome reorganization. Complete identification of TEs is essential for the study of genome evolution. However, the TEs of sweet potato are still poorly understood because of its complex hexaploid genome and difficulty in genome sequencing. The recent availability of the sweet potato transcriptome databases provides an opportunity for discovering and characterizing the expressed TEs. We first established the integrated-transcriptome database by de novo assembling four published sweet potato transcriptome databases from three cultivars in China. Using sequence-similarity search and analysis, a total of 1,405 TEs including 883 retrotransposons and 522 DNA transposons were predicted and categorized. Depending on mapping sets of RNA-Seq raw short reads to the predicted TEs, we compared the quantities, classifications and expression activities of TEs inter- and intra-cultivars. Moreover, the differential expressions of TEs in seven tissues of Xushu 18 cultivar were analyzed by using Illumina digital gene expression (DGE) tag profiling. It was found that 417 TEs were expressed in one or more tissues and 107 in all seven tissues. Furthermore, the copy number of 11 transposase genes was determined to be 1-3 copies in the genome of sweet potato by Real-time PCR-based absolute quantification. Our result provides a new method for TE searching on species with transcriptome sequences while lacking genome information. The searching, identification and expression analysis of TEs will provide useful TE information in sweet potato, which are valuable for the further studies of TE-mediated gene mutation and optimization in asexual reproduction. It contributes to elucidating the roles of TEs in genome evolution.

  4. Characterization and development of EST-SSR markers in sweet potato (Ipomoea batatas (L.) Lam).

    Science.gov (United States)

    Kim, Jin-Hee; Kim, Jun-Hoi; Jo, Won-Sam; Ham, Jeong-Gwan; Chung, Il Kyung; Kim, Kyung-Min

    2016-12-01

    In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The lengths of the developed EST-SSR markers ranged from 100 to 499 bp (average length 238 bp). Their motif sequence types were varied, with most being dinucleotides and pentanucleotides, and the most commonly found motifs were CAGAAT (29.0%) and TCT (2.8%). Based on these SSR-containing sequences, 619 pairs of high-quality SSR primers were designed using WebSat and Primer3web. The total number of primers designed was 144. Polymorphism was evident in 82 EST-SSR markers among 20 Korean sweet potato cultivars tested and in 90 EST-SSR markers in the two parents of a mapping population, Yeseumi and Annobeny. In this study, the hexaploid sweet potato (2n = 6x = 90) EST-SSR markers were developed in the absence of full-sequence data. Moreover, by acting as a molecular tag for particular traits, the EST-SSR marker can also simultaneously identify information about the corresponding gene. These EST-SSR markers will allow the molecular analysis of sweet potato to be done more efficiently. Thus, we can develop high-quality sweet potato while overcoming the challenges from climate change and other unfavorable conditions.

  5. Mycoflora of sun-dried sweet potato ( Ipomoea batatas L .) slices in ...

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    A study was carried out to isolate and quantify the fungi present in sun-dried sweet potato slices in Benin City, Nigeria. Potato tubers were peeled, washed, sliced and sun-dried for 30 days. Oven-dried slices served as control. Meteorological data were obtained for the period of study. Fungal colonies on slices were counted ...

  6. Studies on mutation breeding in sweet potato (Ipomoea batatas (L.) Lam.)

    International Nuclear Information System (INIS)

    Kukimura, H.; Kouyama, Y.

    1982-01-01

    Different genotypes were subjected to gamma rays and EMS to examine the effects on tuber skin colour mutation. Different mutation rates were obtained according to the genotypes. The gamma irradiation induced larger sector size of skin colour mutation than EMS. Gamma rays had an effect on inducing flowering in MV 1 which is utilized in cross breeding. Mutagenic treatment by gamma rays and EMS on the hybrid true seed which segregates in a Mendelian ratio for pigmentation in leaf, stem and tuber and for shape of leaf gave some bias to their segregation ratios. Effects of gamma-ray irradiation on quantitative characters, such as dry matter content and total sugar content in tubers, were also investigated in hybrid populations. The treatments enlarged genetic variations on both the characters, being more effective on total sugar content. Clonal progenies derived from mutagenic treatment by gamma rays and EI were investigated for their quantitative MV 4 -MV 6 characters (tuber yield, dry matter content and total sugar content) in MV 4 -MV 6 . Heritabilities in a broad sense and phenotypic variances were estimated from the measurements on derivative strains obtained by random selection from mutagenic treatment plots. Artificial selection was effective only for tuber yield. Mutant clones with short stem length decreased their tuber yield and vice versa. A few mutant clones were found to excel the originals in dry matter content and total sugar content. Some aspects of mutation breeding in sweet potato are also discussed

  7. Susceptibility of sweet potato (Ipomoea batatas) peel proteins to digestive enzymes.

    Science.gov (United States)

    Maloney, Katherine P; Truong, Van-Den; Allen, Jonathan C

    2014-07-01

    Sweet potato proteins have been shown to possess antioxidant and antidiabetic properties in vivo. The ability of a protein to exhibit systemic effects is somewhat unusual as proteins are typically susceptible to digestive enzymes. This study was undertaken to better understand how digestive enzymes affect sweet potato proteins. Two fractions of industrially processed sweet potato peel, containing 6.8% and 8.5% protein and 80.5% and 83.3% carbohydrate, were used as a source of protein. Sweet potato proteins were incubated with pepsin, trypsin, and chymotrypsin and protein breakdown was visualized with SDS-PAGE. After pepsin digestion, samples were assayed for amylase inhibitory activity. Sporamin, the major storage protein in sweet potatoes, which functions as a trypsin inhibitor as well, exhibited resistance to pepsin, trypsin, and chymotrypsin. Sporamin from blanched peel of orange sweet potatoes was less resistant to pepsin digestion than sporamin from outer peel and from extract of the white-skinned Caiapo sweet potato. Trypsin inhibitory activity remained after simulated gastric digestion, with the Caiapo potato protein and peel samples exhibiting higher inhibitory activity compared to the blanched peel sample. Amylase and chymotrypsin inhibitory activity was not present in any of the samples after digestion.

  8. in vitro production of virus free sweet potato [ipomoea batatas (l

    African Journals Online (AJOL)

    preferred customer

    parallel to and spatially linked with mapped normal faults. This is consistent with the overall sub- latitudinal extension direction in the Quaternary deduced from fault slip analysis and earthquake focal mechanism solutions. Out of the rift axis, pre-existing faults were apparently reactivated and acted as conduits for magmatism ...

  9. in vitro production of virus free sweet potato [ipomoea batatas (l

    African Journals Online (AJOL)

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    ABSTRACT: An AFLP analysis was carried out to study the genetic diversity of Colletotrichum sublineolum .... To further elucidate the genetic relationship .... Alemayehu Chala. T ab le 3. Dice S im ila rity co e fficie n. t a m o n g. 2. 2 iso la te. s o f C . Sub lineolum fro m a sin g le fie ld in so u th e rn. E th io p ia . Isola tes. 2. 1.

  10. Sweetpotato Stem Cuttings Database in Preparation for Flight

    Science.gov (United States)

    Mortley, Desmond G.

    2002-01-01

    Since 1985, Tuskegee University has been engaged in research that addresses NASA's mission to achieve long-duration human space exploration on the moon and Mars. The successful research of Dr. George Washington Carver, the beginning of our long history of working with crops for food and industrial uses, is well known. This history and contemporary technical expertise in food/industrial crops, and our interest in pursuing new, interdisciplinary approaches to education and research led us to submit a proposal to NASA in 1985 to pursue the hydroponic production of sweetpotato for food for astronaut-explorers on future long-term, distant space missions. As a carbohydrate and dual vegetable (edible leaves), the versatile sweetpotato has the potential to be a key crop as a main source of energy and other nutrients such as beta-carotene, calcium and potassium. Sweetpotato is a crop very suited to space, as was documented in a monograph published by our scientists in 1984.

  11. Sweepoviruses Cause Disease in Sweet Potato and Related Ipomoea spp.: Fulfilling Koch's Postulates for a Divergent Group in the Genus Begomovirus

    Science.gov (United States)

    Márquez-Martín, Belén; Moriones, Enrique; Navas-Castillo, Jesús

    2011-01-01

    Sweet potato (Ipomoea batatas) and related Ipomoea species are frequently infected by monopartite begomoviruses (genus Begomovirus, family Geminiviridae), known as sweepoviruses. Unlike other geminiviruses, the genomes of sweepoviruses have been recalcitrant to rendering infectious clones to date. Thus, Koch's postulates have not been fullfilled for any of the viruses in this group. Three novel species of sweepoviruses have recently been described in Spain: Sweet potato leaf curl Lanzarote virus (SPLCLaV), Sweet potato leaf curl Spain virus (SPLCSV) and Sweet potato leaf curl Canary virus (SPLCCaV). Here we describe the generation of the first infectious clone of an isolate (ES:MAL:BG30:06) of SPLCLaV. The clone consisted of a complete tandem dimeric viral genome in a binary vector. Successful infection by agroinoculation of several species of Ipomoea (including sweet potato) and Nicotiana benthamiana was confirmed by PCR, dot blot and Southern blot hybridization. Symptoms observed in infected plants consisted of leaf curl, yellowing, growth reduction and vein yellowing. Two varieties of sweet potato, ‘Beauregard’ and ‘Promesa’, were infected by agroinoculation, and symptoms of leaf curl and interveinal loss of purple colouration were observed, respectively. The virus present in agroinfected plants was readily transmitted by the whitefly Bemisia tabaci to I. setosa plants. The progeny virus population present in agroinfected I. setosa and sweet potato plants was isolated and identity to the original isolate was confirmed by sequencing. Therefore, Koch's postulates were fulfilled for the first time for a sweepovirus. PMID:22073314

  12. Sweepoviruses cause disease in sweet potato and related Ipomoea spp.: fulfilling Koch's postulates for a divergent group in the genus begomovirus.

    Directory of Open Access Journals (Sweden)

    Helena P Trenado

    Full Text Available Sweet potato (Ipomoea batatas and related Ipomoea species are frequently infected by monopartite begomoviruses (genus Begomovirus, family Geminiviridae, known as sweepoviruses. Unlike other geminiviruses, the genomes of sweepoviruses have been recalcitrant to rendering infectious clones to date. Thus, Koch's postulates have not been fullfilled for any of the viruses in this group. Three novel species of sweepoviruses have recently been described in Spain: Sweet potato leaf curl Lanzarote virus (SPLCLaV, Sweet potato leaf curl Spain virus (SPLCSV and Sweet potato leaf curl Canary virus (SPLCCaV. Here we describe the generation of the first infectious clone of an isolate (ES:MAL:BG30:06 of SPLCLaV. The clone consisted of a complete tandem dimeric viral genome in a binary vector. Successful infection by agroinoculation of several species of Ipomoea (including sweet potato and Nicotiana benthamiana was confirmed by PCR, dot blot and Southern blot hybridization. Symptoms observed in infected plants consisted of leaf curl, yellowing, growth reduction and vein yellowing. Two varieties of sweet potato, 'Beauregard' and 'Promesa', were infected by agroinoculation, and symptoms of leaf curl and interveinal loss of purple colouration were observed, respectively. The virus present in agroinfected plants was readily transmitted by the whitefly Bemisia tabaci to I. setosa plants. The progeny virus population present in agroinfected I. setosa and sweet potato plants was isolated and identity to the original isolate was confirmed by sequencing. Therefore, Koch's postulates were fulfilled for the first time for a sweepovirus.

  13. Identification of relevant non-target organisms exposed to weevil-resistant Bt sweetpotato in Uganda.

    Science.gov (United States)

    Rukarwa, R J; Mukasa, S B; Odongo, B; Ssemakula, G; Ghislain, M

    2014-06-01

    Assessment of the impact of transgenic crops on non-target organisms (NTO) is a prerequisite to their release into the target environment for commercial use. Transgenic sweetpotato varieties expressing Cry proteins (Bt sweetpotato) are under development to provide effective protection against sweetpotato weevils (Coleoptera) which cause severe economic losses in sub-Saharan Africa. Like any other pest control technologies, genetically engineered crops expressing insecticidal proteins need to be evaluated to assess potential negative effects on non-target organisms that provide important services to the ecosystem. Beneficial arthropods in sweetpotato production systems can include pollinators, decomposers, and predators and parasitoids of the target insect pest(s). Non-target arthropod species commonly found in sweetpotato fields that are related taxonomically to the target pests were identified through expert consultation and literature review in Uganda where Bt sweetpotato is expected to be initially evaluated. Results indicate the presence of few relevant non-target Coleopterans that could be affected by Coleopteran Bt sweetpotato varieties: ground, rove and ladybird beetles. These insects are important predators in sweetpotato fields. Additionally, honeybee (hymenoptera) is the main pollinator of sweetpotato and used for honey production. Numerous studies have shown that honeybees are unaffected by the Cry proteins currently deployed which are homologous to those of the weevil-resistant Bt sweetpotato. However, because of their feeding behaviour, Bt sweetpotato represents an extremely low hazard due to negligible exposure. Hence, we conclude that there is good evidence from literature and expert opinion that relevant NTOs in sweetpotato fields are unlikely to be affected by the introduction of Bt sweetpotato in Uganda.

  14. Horizontal Transmission of "Candidatus Liberibacter solanacearum" by Bactericera cockerelli (Hemiptera: Triozidae on Convolvulus and Ipomoea (Solanales: Convolvulaceae.

    Directory of Open Access Journals (Sweden)

    Glenda L Torres

    Full Text Available "Candidatus Liberibacter solanacearum" (Proteobacteria is an important pathogen of solanaceous crops (Solanales: Solanaceae in North America and New Zealand, and is the putative causal agent of zebra chip disease of potato. This phloem-limited pathogen is transmitted to potato and other solanaceous plants by the potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae. While some plants in the Convolvulaceae (Solanales are also known hosts for B. cockerelli, previous efforts to detect Liberibacter in Convolvulaceae have been unsuccessful. Moreover, studies to determine whether Liberibacter can be acquired from these plants by B. cockerelli are lacking. The goal of this study was to determine whether horizontal transmission of Liberibacter occurs among potato psyllids on two species of Convolvulaceae, sweet potato (Ipomoea batatas and field bindweed (Convolvulus arvensis, which grows abundantly in potato growing regions of the United States. Results indicated that uninfected psyllids acquired Liberibacter from both I. batatas and C. arvensis if infected psyllids were present on plants concurrently with the uninfected psyllids. Uninfected psyllids did not acquire Liberibacter from plants if the infected psyllids were removed from the plants before the uninfected psyllids were allowed access. In contrast with previous reports, PCR did detect the presence of Liberibacter DNA in some plants. However, visible amplicons were faint and did not correspond with acquisition of the pathogen by uninfected psyllids. None of the plants exhibited disease symptoms. Results indicate that horizontal transmission of Liberibacter among potato psyllids can occur on Convolvulaceae, and that the association between Liberibacter and Convolvulaceae merits additional attention.

  15. Horizontal Transmission of "Candidatus Liberibacter solanacearum" by Bactericera cockerelli (Hemiptera: Triozidae) on Convolvulus and Ipomoea (Solanales: Convolvulaceae).

    Science.gov (United States)

    Torres, Glenda L; Cooper, W Rodney; Horton, David R; Swisher, Kylie D; Garczynski, Stephen F; Munyaneza, Joseph E; Barcenas, Nina M

    2015-01-01

    "Candidatus Liberibacter solanacearum" (Proteobacteria) is an important pathogen of solanaceous crops (Solanales: Solanaceae) in North America and New Zealand, and is the putative causal agent of zebra chip disease of potato. This phloem-limited pathogen is transmitted to potato and other solanaceous plants by the potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae). While some plants in the Convolvulaceae (Solanales) are also known hosts for B. cockerelli, previous efforts to detect Liberibacter in Convolvulaceae have been unsuccessful. Moreover, studies to determine whether Liberibacter can be acquired from these plants by B. cockerelli are lacking. The goal of this study was to determine whether horizontal transmission of Liberibacter occurs among potato psyllids on two species of Convolvulaceae, sweet potato (Ipomoea batatas) and field bindweed (Convolvulus arvensis), which grows abundantly in potato growing regions of the United States. Results indicated that uninfected psyllids acquired Liberibacter from both I. batatas and C. arvensis if infected psyllids were present on plants concurrently with the uninfected psyllids. Uninfected psyllids did not acquire Liberibacter from plants if the infected psyllids were removed from the plants before the uninfected psyllids were allowed access. In contrast with previous reports, PCR did detect the presence of Liberibacter DNA in some plants. However, visible amplicons were faint and did not correspond with acquisition of the pathogen by uninfected psyllids. None of the plants exhibited disease symptoms. Results indicate that horizontal transmission of Liberibacter among potato psyllids can occur on Convolvulaceae, and that the association between Liberibacter and Convolvulaceae merits additional attention.

  16. Survey of genome sequences in a wild sweet potato, Ipomoea trifida (H. B. K.) G. Don

    Science.gov (United States)

    Hirakawa, Hideki; Okada, Yoshihiro; Tabuchi, Hiroaki; Shirasawa, Kenta; Watanabe, Akiko; Tsuruoka, Hisano; Minami, Chiharu; Nakayama, Shinobu; Sasamoto, Shigemi; Kohara, Mitsuyo; Kishida, Yoshie; Fujishiro, Tsunakazu; Kato, Midori; Nanri, Keiko; Komaki, Akiko; Yoshinaga, Masaru; Takahata, Yasuhiro; Tanaka, Masaru; Tabata, Satoshi; Isobe, Sachiko N.

    2015-01-01

    Ipomoea trifida (H. B. K.) G. Don. is the most likely diploid ancestor of the hexaploid sweet potato, I. batatas (L.) Lam. To assist in analysis of the sweet potato genome, de novo whole-genome sequencing was performed with two lines of I. trifida, namely the selfed line Mx23Hm and the highly heterozygous line 0431-1, using the Illumina HiSeq platform. We classified the sequences thus obtained as either ‘core candidates’ (common to the two lines) or ‘line specific’. The total lengths of the assembled sequences of Mx23Hm (ITR_r1.0) was 513 Mb, while that of 0431-1 (ITRk_r1.0) was 712 Mb. Of the assembled sequences, 240 Mb (Mx23Hm) and 353 Mb (0431-1) were classified into core candidate sequences. A total of 62,407 (62.4 Mb) and 109,449 (87.2 Mb) putative genes were identified, respectively, in the genomes of Mx23Hm and 0431-1, of which 11,823 were derived from core sequences of Mx23Hm, while 28,831 were from the core candidate sequence of 0431-1. There were a total of 1,464,173 single-nucleotide polymorphisms and 16,682 copy number variations (CNVs) in the two assembled genomic sequences (under the condition of log2 ratio of >1 and CNV size >1,000 bases). The results presented here are expected to contribute to the progress of genomic and genetic studies of I. trifida, as well as studies of the sweet potato and the genus Ipomoea in general. PMID:25805887

  17. Survey of genome sequences in a wild sweet potato, Ipomoea trifida (H. B. K.) G. Don.

    Science.gov (United States)

    Hirakawa, Hideki; Okada, Yoshihiro; Tabuchi, Hiroaki; Shirasawa, Kenta; Watanabe, Akiko; Tsuruoka, Hisano; Minami, Chiharu; Nakayama, Shinobu; Sasamoto, Shigemi; Kohara, Mitsuyo; Kishida, Yoshie; Fujishiro, Tsunakazu; Kato, Midori; Nanri, Keiko; Komaki, Akiko; Yoshinaga, Masaru; Takahata, Yasuhiro; Tanaka, Masaru; Tabata, Satoshi; Isobe, Sachiko N

    2015-04-01

    Ipomoea trifida (H. B. K.) G. Don. is the most likely diploid ancestor of the hexaploid sweet potato, I. batatas (L.) Lam. To assist in analysis of the sweet potato genome, de novo whole-genome sequencing was performed with two lines of I. trifida, namely the selfed line Mx23Hm and the highly heterozygous line 0431-1, using the Illumina HiSeq platform. We classified the sequences thus obtained as either 'core candidates' (common to the two lines) or 'line specific'. The total lengths of the assembled sequences of Mx23Hm (ITR_r1.0) was 513 Mb, while that of 0431-1 (ITRk_r1.0) was 712 Mb. Of the assembled sequences, 240 Mb (Mx23Hm) and 353 Mb (0431-1) were classified into core candidate sequences. A total of 62,407 (62.4 Mb) and 109,449 (87.2 Mb) putative genes were identified, respectively, in the genomes of Mx23Hm and 0431-1, of which 11,823 were derived from core sequences of Mx23Hm, while 28,831 were from the core candidate sequence of 0431-1. There were a total of 1,464,173 single-nucleotide polymorphisms and 16,682 copy number variations (CNVs) in the two assembled genomic sequences (under the condition of log2 ratio of >1 and CNV size >1,000 bases). The results presented here are expected to contribute to the progress of genomic and genetic studies of I. trifida, as well as studies of the sweet potato and the genus Ipomoea in general. © The Author 2015. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

  18. Prevalence and implications of sweetpotato recovery from sweet ...

    African Journals Online (AJOL)

    Sweet potato virus disease (SPVD) is the most important disease of sweetpotato in the tropics. It causes yield losses of up to 98% and extinction of elite cultivars. Although there are no reports of immune cultivars, disease recovery phenomenon (a manifestation of some form of resistance) was reported in many vegetatively ...

  19. Determinants of marketing efficiency for sweetpotato in Nasarawa ...

    African Journals Online (AJOL)

    The study was conducted to assess the determinants of sweet potato marketing efficiency in Nasarawa State and FCT. Multistage sampling method was used for the study. One hundred and twenty sweetpotato marketers were randomly selected from the eight markets of Nasarawa State and FCT. Structured questionnaire ...

  20. Farmers' willingness to pay for quality orange fleshed sweetpotato ...

    African Journals Online (AJOL)

    The special nutrition need by people have shifted their focus to the adoption of Orange Flesh Sweet Potato for cumption due to its high content of Vitamin A. Sweetpotato which is one of the most important but underutilized food crops in the world have now attracted concerted efforts globally to in the past decade to feed the ...

  1. Advances in breeding for sweetpotato virus resitance in Uganda

    African Journals Online (AJOL)

    be officially released in Uganda, where sweetpotato is an important food crop. The newly released cultiva rs, five of them ... Uganda's human population is roughly I 8 million (World. Bank, 1995) indicating an annual per ... polycrosses that are open pollinated mainly by bees and hand crosses of specific male and female ...

  2. Evaluation of promising sweetpotato genotypes for high altitude ...

    African Journals Online (AJOL)

    The trials were set up to identify sweetpotato genotypes with adaptation to highland agroecologies with special reference to resistance to Ahemaria blight ... growth and at harvest, four genotypes and the local check, Magabari, bad high levels of resistance toA/Jemaria blight. Eight genotypes had total storage root yield ...

  3. Evaluation of the suitability of cassava and sweetpotato flours for ...

    African Journals Online (AJOL)

    The results showed that water binding capacity, solubility and swelling power affect the overall quality of pasta. Pasta made from sweetpotato composite flour was too brittle and crumbled easily when pressed between the fingers. Pasta made from 50% cassava (Abasafita)/50% hard wheat performed better and showed no ...

  4. Acceptability of bread produced from hausa-potato and sweetpotato ...

    African Journals Online (AJOL)

    This study was conducted to compare the quality of bread produced from Hausa potato (HP) flour and sweetpotato (SP) flour at different substitution levels with wheat flour. The proximate analysis and sensory evaluation of the bread samples were determined. The proximate composition analysis revealed that the ...

  5. Participatory consumer evaluation of twelve sweetpotato varieties in ...

    African Journals Online (AJOL)

    STORAGESEVER

    2010-03-15

    Mar 15, 2010 ... perception for evaluating storage roots for consumer preference. The farmers set their own criteria (consumer preference, taste, aroma, market preference and overall acceptance) for evaluation. During the evaluation process, farmers evaluated two replications of all the twelve sweetpotato varieties per ...

  6. Reaction of sweetpotato clones to virus disease and their yield ...

    African Journals Online (AJOL)

    Although among the available control measures use of resistant and high yielding varieties is the cheapest and effective means of controlling the disease, reaction of several sweetpotato clones to the disease is largely unknown. A study was conducted in three geographical regions (Namulonge, Kachwekano, Bulegeni, ...

  7. Assessment of the extent of adoption of sweetpotato production ...

    African Journals Online (AJOL)

    Ekwuruchi O.Mbanaso

    ASSESSMENT OF THE EXTENT OF ADOPTION OF SWEETPOTATO. PRODUCTION TECHNOLOGY BY FARMERS IN THE SOUTHEAST AGRO-. ECOLOGICAL ZONE OF NIGERIA. 1. MBANASO, E. O., A. E. AGWU. 2. , A. C. ANYANWU. 3. AND G. N. ASUMUGHA. 1. 1. National Root Crops Research Institute, Umudike. 2.

  8. Relative abundance of sweetpotato whitefly in orange-fleshed ...

    African Journals Online (AJOL)

    ACSS

    and one white fleshed (TIS87/0087) as a check at National Root Crops Research Institute (NRCRI),. Umudike ... sweetpotato (OFSP) is due mainly to the nutritional and health advantages in its high vitamin A content. Vitamin A deficiency affects over 144 million children under five ... transmission of a variety of plant viruses.

  9. Total β-carotene content of orange sweetpotato cultivated under ...

    African Journals Online (AJOL)

    MFaber

    harvesting at four months (warm climates) to six months. (temperate climates) after planting (Niederwieser, 2004). In many traditional settings sweetpotato is harvested as needed (a practice called piece meal harvesting). When the roots are kept in the soil until needed, the carotenoid and β-carotene content of the roots may ...

  10. Occurrence of white rust (Albugo ipomoeae-panduratae on Ipomoea acuminata in the brazilian mid-west

    Directory of Open Access Journals (Sweden)

    A.P.S Pagani

    2012-03-01

    Full Text Available Spontaneous plants of Ipomoea acuminata ("morning glory" exhibiting white rust pustules were found in a field crop area of Planaltina, DF, in the fall season of 2010 and the disease causal agent was identified as Albugo ipomoea-panduratae (Oomycota. No reports of the association between A. ipomoea-panduratae and I. acuminata were known in Brazil previously to 2010. A reference specimen was deposited at the University of Brasilia Mycological Reference Collection.

  11. Is the begomovirus, sweet potato leaf curl virus, really seed transmitted in sweetpotato?

    Science.gov (United States)

    Sweetpotato is one of the major root crops in the world and is also widely grown in the southern United States. Sweet potato leaf curl virus (SPLCV) is a begomovirus posing a serious threat to sweetpotato production worldwide and is primarily transmitted by whitefly (Bemisia tabaci) or through veget...

  12. Sistemas de labranza y densidades de la batata: calidad del suelo y de las raíces tuberosas.

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    Maiby Pérez-Darniz

    2016-12-01

    Full Text Available El objetivo de este trabajo fue determinar el efecto de sistemas de labranza y densidades de siembra en la calidad de las raíces tuberosas de batata (Ipomoea batata (L Lamb.. El estudio se realizó en la Universidad Central de Venezuela, en el año 2014. Se determinaron los grados de severidad de la degradación del suelo y su correspondencia con las escalas de preferencia basadas en el peso unitario y dimensiones de la raíz tuberosa. Se usó un arreglo en franjas, donde los sistemas de labranza (SL fueron asignados a las parcelas, y las densidades de siembra (Ds a las franjas. Los SL consistieron en labranza vertical con cincel (LC, con arado de disco (LA, convencional con tres pases de rastra de disco (LCo y mínima con un pase de rastra de disco (LM. Las Ds fueron de 0,25 cm (D1 y 0,15 cm (D2 en la hilera. La calidad física del suelo fue superior en los tratamientos de LA. De estos, la combinación con la distancia de siembra más larga (LA-D1 favoreció la mayor calidad postcosecha del cultivo. Al contrario, en los tratamientos con cincel se promovió la menor aceptación del consumidor. La ligera a moderada severidad de la degradación del suelo no explicó la variación de la calidad postcosecha. Se in ere sobre la menor tasa de movimiento de agua en LC, que promovió un exceso de humedad y la más baja calidad de la raíz tuberosa.

  13. Evaluation of South African bred cultivars of sweetpotato under ...

    African Journals Online (AJOL)

    RÉSUMÉ La patate douce (Ipomea batatas) est largement produite et consommée au Botwana. L'identification des variétés adaptées aux conditions climatiques locales peut stimuler la production et donner aux producteurs et aux consommateurs une un choix dans une gamme des qualités des tubercules. Comme le pays ...

  14. First field collection of the Rough Sweetpotato Weevil, Blosyrus asellus(Olivier)(Coleoptera: Curculionidae) on Hawaii Island, with notes on detection methods

    Science.gov (United States)

    Rough sweetpotato weevil, Blosyrus asellus(Olivier)(Coleoptera: Curculionidae), was first detected in the state of Hawaii at a commercial Okinawan sweetpotato farm in Waipio, Oahu, on 14 November 2008. Reported here is, the first detection of this pest in sweetpotato fields on the island of Hawaii (...

  15. Escuela de campo de agricultores para el fitomejoramiento participativo del boniato (Ipomoea batatas (L. Lam.. Farmer´s Field School for the sweet potato (Ipomoea batatas (L. Lam. participatory breeding.

    Directory of Open Access Journals (Sweden)

    Mario Díaz López

    2013-08-01

    Full Text Available Resumen.Se presenta una Escuela de Campo para capacitar a los agricultores de sub urbanos en el Fitomejoramiento Participativo del boniato. En su concepción se emplearon métodos del nivel teórico (análisis – síntesis, inducción - deducción, histórico y lógico, así como del nivel empírico (entrevistas, entrevistas a grupos focales, análisis de documentos, observación, encuestas que permitieron integrar el Fitomejoramiento Participativo con la capacitación de los agricultores. Se discuten los aspectos metodológicos relacionados con su aplicación. Abstract. A Farmer´s Field School is presented to train farmers in the sub urban Participatory Plant Breeding of sweet potato. And the empirical level (interviews, focus group interviews, document analysis, observation, surveys that allowed integrating Participatory Plant Breeding in concept with theoretical methods (analysis,- synthesis, induction -deduction, historical and logical analysis were used training of farmers. methodological issues related to its implementation are discussed.

  16. Escuela de campo de agricultores para el fitomejoramiento participativo del boniato (Ipomoea batatas (L.) Lam.). Farmer´s Field School for the sweet potato (Ipomoea batatas (L.) Lam.) participatory breeding.

    OpenAIRE

    Mario Díaz López

    2013-01-01

    Resumen.Se presenta una Escuela de Campo para capacitar a los agricultores de sub urbanos en el Fitomejoramiento Participativo del boniato. En su concepción se emplearon métodos del nivel teórico (análisis – síntesis, inducción - deducción, histórico y lógico), así como del nivel empírico (entrevistas, entrevistas a grupos focales, análisis de documentos, observación, encuestas que permitieron integrar el Fitomejoramiento Participativo con la capacitación de los agricultores. Se discuten lo...

  17. Digestibility and structural properties of thermal and high hydrostatic pressure treated sweet potato (Ipomoea batatas L.) protein.

    Science.gov (United States)

    Sun, Minjie; Mu, Taihua; Sun, Hongnan; Zhang, Miao

    2014-09-01

    This study assessed the effects of thermal (40, 60, 80, 100 and 127 °C) and high hydrostatic pressure (HHP, 200, 400 and 600 MPa) treatments on the in vitro digestibility and structural properties of sweet potato protein (SPP). The results showed that the in vitro digestibility of SPP increased significantly with increasing heating temperature and heating time (0-60 min), while HHP treatment had little or no effect. Native SPP denaturation temperature (T d ) and enthalpy change (ΔH) were 89.0 °C and 9.6 J/g, respectively. Thermal and HHP treated SPP had T d of 84.6-88.9 °C and 86.4-87.6 °C, respectively. ΔH of thermal treated SPP was 3.6-6.4 J/g, while that of HHP treated SPP was 5.9-7.8 J/g. The differential scanning calorimetry (DSC) results demonstrated that HHP and thermal treatments both significantly reduced SPP thermodynamic stability. Circular dichroism analyses revealed that native SPP contains α-helixes, β-sheets and random coils (4.3, 48.0 and 47.7%, respectively). After thermal treatment at 127 °C for 20 min, the content of α-helixes and turns increased significantly (13.2 and 27.6%, respectively), whereas the content of β-sheets decreased significantly (12.3%). In contrast, HHP treatment increased the content of β-sheets, but decreased the content of random coils. This study suggested that the SPP structure changes might be the main reason affecting the in vitro digestibility of SPP, and thermal treatment was more effective at changing SPP secondary structures and improving in vitro SPP digestibility than HHP treatment.

  18. Comparative analysis of phytochemicals and nutrient availability in two contrasting cultivars of sweet potato (Ipomoea batatas L.).

    Science.gov (United States)

    Shekhar, Shubhendu; Mishra, Divya; Buragohain, Alak Kumar; Chakraborty, Subhra; Chakraborty, Niranjan

    2015-04-15

    Sweet potato ranks as the world's seventh most important food crop, and has major contribution to energy and phytochemical source of nutrition. To unravel the molecular basis for differential nutrient availability, and to exploit the natural genetic variation(s) of sweet potato, a series of physiochemical and proteomics experiment was conducted using two contrasting cultivars, an orange-fleshed sweet potato (OFSP) and a white-fleshed sweet potato (WFSP). Phytochemical screening revealed high percentage of carbohydrate, reducing sugar and phenolics in WFSP, whereas OFSP showed increased levels of total protein, flavonoids, anthocyanins, and carotenoids. The rate of starch and cellulose degradation was found to be less in OFSP during storage, indicating tight regulation of gene(s) responsible for starch-degradation. Comparative proteomics displayed a cultivar-dependent expression of proteins along with evolutionarily conserved proteins. These results suggest that cultivar-specific expression of proteins and/or their interacting partners might play a crucial role for nutrient acquisition in sweet potato. Copyright © 2014 Elsevier Ltd. All rights reserved.

  19. Field studies of sweet potatoes and cowpeas in response to elevated carbon dioxide. Progress report. [Ipomoea batatas; Vigna unguiculata

    Energy Technology Data Exchange (ETDEWEB)

    Biswas, P.K.

    1985-01-01

    The physiological and biochemical effects of enriched CO/sub 2/ on sweet potatoes and cowpeas were studied in open top chambers. The main task was to assemble equipment to generate test atmospheres of CO/sub 2/ in the field, using open top chambers as the basic exposure unit for studying the response of sweet potatoes and cowpeas to enriched CO/sub 2/. Plant responses to CO/sub 2/ in open top chambers have been demonstrated by several investigators in a variety of crops. This study focused on growth and development of both sweet potatoes and cowpeas at levels of CO/sub 2/ ranging from the ambient level of 354 ppM to 659 ppM. The effects of CO/sub 2/ on leaf and stem weights, stem length, leaf area, and stomatal number and conductance were studied on both species. Additional studies on sweet potatoes included the effects of CO/sub 2/ on the weight, chemical content and quality of tubers. Additional studies on cowpeas included the effects of CO/sub 2/ on the weight of seeds and rate of nitrogen fixation. 27 figs., 35 tabs. (ACR)

  20. Comparison of the effectiveness of various pretreatment methods on the enzymatic hydrolysis of sweet potato (Ipomoea batatas L. ) biomass

    Energy Technology Data Exchange (ETDEWEB)

    Bhattacharya, S.; Biswas, P.K.; Tolbert, M.E.M.

    1987-01-01

    Lignin, phenolic substances and other interfering constituents in agricultural biomass act as physical barriers and diminish the rate and extent of hydrolytic degradation of cellulose. In order to enhance the enzymatic susceptibility of cellulose substrate, a variety of pretreatment processes prior to cellulose hydrolysis have been investigated. Waste leaves and stems of sweet potatoes have been examined as substrates for the production of fermentable sugars for conversion to ethanol. This investigation considers the effects of a variety of single and multiple pretreatment methods on the rate of enzymatic hydrolysis of stems and leaves by cellulase from Trichoderma viride. For the single pretreatments, each substrate was treated with either 2.0% or 4.0% (w/v) sodium hydroxide at room temperature (24 h), or with 70% (v/v) ethanol at 60C (3h), or with methanol, or was extracted directly in 75% zinc chloride (w/v) solution in 0.5% hydrochloric acid at 140C (12 min). Multiple pretreatments consisted of the following: methanol or methanol and acetone, sulfuric acid (1%, v/v) followed by sodium hydroxide (4.0%, w/v) or zinc chloride or sodium hydroxide and zinc chloride; ethanol, zinc chloride; methanol or methanol and acetone followed by zinc chloride. Single pretreatments were not promising. However, single or multiple pretreatments of leaves enhanced the enzymatic hydrolysis and yields of glucose. Neither single nor multiple pretreatment was found to be very effective for stem substrates, as untreated stems were highly susceptible to cellulolysis. Among all the pretreatments, those involving zinc chloride in hydrochloric acid generally resulted in higher glucose yields. 18 references.

  1. De novo sequencing and comprehensive analysis of the mutant transcriptome from purple sweet potato (Ipomoea batatas L.).

    Science.gov (United States)

    Ma, Peiyong; Bian, Xiaofeng; Jia, Zhaodong; Guo, Xiaoding; Xie, Yizhi

    2016-01-10

    Purple sweet potatoes, rich in anthocyanin, have been widely favored in light of increasing awareness of health and food safety. In this study, a mutant of purple sweet potato (white peel and flesh) was used to study anthocyanin metabolism by high-throughput RNA sequencing and comparative analysis of the mutant and wild type transcriptomes. A total of 88,509 unigenes ranging from 200nt to 14,986nt with an average length of 849nt were obtained. Unigenes were assigned to Gene Ontology (GO), Clusters of Orthologous Group (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Functional enrichment using GO and KEGG annotations showed that 3828 of the differently expressed genes probably influenced many important biological and metabolic pathways, including anthocyanin biosynthesis. Most importantly, the structural and transcription factor genes that contribute to anthocyanin biosynthesis were downregulated in the mutant. The unigene dataset that was used to discover the anthocyanin candidate genes can serve as a comprehensive resource for molecular research in sweet potato. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Purple sweet potato (Ipomoea batatas L.) anthocyanins: preventive effect on acute and subacute alcoholic liver damage and dealcoholic effect.

    Science.gov (United States)

    Sun, Hongnan; Mu, Taihua; Liu, Xingli; Zhang, Miao; Chen, Jingwang

    2014-03-19

    This study aimed to investigate the dealcoholic effect and preventive effect of anthocyanins from purple sweet potato (PSPAs) on acute and subacute alcoholic liver damage (ALD). Seven-week-old male inbred mice were grouped into five groups: control group (without PSPAs and ethanol treatments), model group (with ethanol treatment only), low-dose group (50 mg PSPAs/kg body weight), middle-dose group (125 mg PSPAs/kg body weight), and high-dose group (375 mg PSPAs/kg body weight), and the mice in all groups were administered intragastrically. Biochemical parameters of serum and liver were determined, and the histopathological changes of liver tissue were also analyzed. Results showed that all tested parameters were ameliorated after consumption of PSPAs. Therefore, PSPAs have preventive effect on acute and subacute ALD. It is suggested that PSPAs could be used as a supplementary reagent during prophylactic and curative managements of ALD.

  3. Characterization and hepatoprotective activity of anthocyanins from purple sweet potato (Ipomoea batatas L. cultivar Eshu No. 8

    Directory of Open Access Journals (Sweden)

    Lin Wang

    2017-07-01

    Full Text Available The hepatoprotective activity of anthocyanin-rich purple sweet potato extract (APSPE was demonstrated. Sixty mice were randomly divided into six groups: control group [without carbon tetrachloride (CCl4 or APSPE]; model group (with CCl4 only; positive control group (50 mg/kg body weight silymarin; low-dose group (100 mg/kg body weight APSPE; medium-dose group (200 mg/kg body weight APSPE; and high-dose group (400 mg/kg body weight APSPE. After 10 days intragastric administration of the respective supplements, the mice in all groups except control were injected intraperitoneally with CCl4 (0.15% in arachis oil, 10 mL/kg body weight, intravenous. Twelve hours after CCl4 injection, the mice were measured in terms of liver index, levels of aspartate aminotransferase and alanine aminotransferase in serum, as well as glutathione, superoxide dismutase, and malondialdehyde in liver homogenate. Additionally, the livers of mice were stained with hematoxylin and eosin and sectioned for observation. Nineteen purple sweet potato anthocyanins were identified from the purple sweet potato cultivar Eshu No. 8 and analyzed by liquid chromatography– electrospray ionization–tandem mass spectrometry. Peonidin 3-coumaryl-p-hydroxybenzoyl sophoroside-5-glucoside was first identified in purple sweet potato. The results showed that anthocyanins in Eshu No. 8 had good hepatoprotective activity.

  4. Enzymatic saccharification of cellulose and cellulosic biomass of sweet potato (Ipomoea batatas L. ): two-step hydrolysis

    Energy Technology Data Exchange (ETDEWEB)

    Tolbert, M.E.M.; Bhattacharya, S.; Biswas, P.K.

    1986-01-01

    The enzymatic hydrolysis of lignocellulosic biomass of sweet potato vines as well as microcrystalline cellulose, Sigmacell type 20, by cellulases from Trichoderma viride is reported. Material was treated with a concentrated solution of ZnCl2 and 0.5% hydrochloric acid and heated at 145C for 12 minutes. Hydrolysis of the cellulose precipitated by acetone was 2-2.5 times greater than untreated lignocellulose and approached 64% conversion. A two-step hydrolysis of treated or untreated cellulose resulted in higher conversion of cellulose into reducing sugars. Increasing cellulase concentrations increased the conversion, but an increase in substrate concentration resulted in a decrease in cellulose conversion. 20 references.

  5. Yield response of ten varieties of sweet potato (Ipomoea batatas L. cultivated on dryland in rainy season

    Directory of Open Access Journals (Sweden)

    Akbar Saitama

    2017-07-01

    Full Text Available Sweet potato is a tuber commodity and one of alternative crops in Indonesia. The demand of sweet potato in Indonesia continues to increase. However, the supply of sweet potatoes for consumption estimated is 2020 in Indonesia will be deficit. Low production of sweet potato is basically due to the decrease of land area as cultivation production and also sweet potatoes have a low yield when planted in rainy season. Based on the high utilization of sweet potato make demand for this commodities continues to increase.Therefore, several strategies to increase crop yields of sweet potato needs to be done. This study aimed to elucidate various sweet potato varieties that can cultivated on dry land in the rainy season. This study was conducted from November 2016 until March 2017 using a randomized block design with treatments of  ten varieties of sweet potato consisting of (V1 Papua Solossa variety, (V2 Jago variety, (V3 Kidal variety, (V4 Antin-1variety, (V5 Sari variety, (V6 Sawentar variety, (V7 Beta-2variety, (V8 Antin-2variety, (V9 Antin-3 variety, (V10 Beta-1variety. The results showed different responses of each variety.The vegetative growth was high as shown by the LAI value of 7.23 at 90 days after planting. In conclusion, the sweet potato leaves had to be prune to boost the agronomic yield. Yields of  ten varieties of sweet potato crops ranged from 8.86 to 44.76 t/ha. Some varieties such as Sari, Papua Salosa and Beta-2 varieties showed high yield although they were planted in moorland conditions in the rainy season.

  6. Altered Phenylpropanoid Metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development.

    Science.gov (United States)

    Wang, Hongxia; Yang, Jun; Zhang, Min; Fan, Weijuan; Firon, Nurit; Pattanaik, Sitakanta; Yuan, Ling; Zhang, Peng

    2016-01-04

    There is no direct evidence of the effect of lignin metabolism on early storage root development in sweet potato. In this study, we found that heterologous expression of the maize leaf color (Lc) gene in sweet potato increased anthocyanin pigment accumulation in the whole plant and resulted in reduced size with an increased length/width ratio, low yield and less starch content in the early storage roots. RT-PCR analysis revealed dramatic up-regulation of the genes involved in the lignin biosynthesis pathway in developing storage roots, leading to greater lignin content in the Lc transgenic lines, compared to the wild type. This was also evidenced by the enhanced lignification of vascular cells in the early storage roots. Furthermore, increased expression of the β-amylase gene in leaves and storage roots also accelerated starch degradation and increased the sugar use efficiency, providing more energy and carbohydrate sources for lignin biosynthesis in the Lc transgenic sweet potato. Lesser starch accumulation was observed in the developing storage roots at the initiation stage in the Lc plants. Our study provides experimental evidence of the basic carbohydrate metabolism underlying the development of storage roots, which is the transformation of lignin biosynthesis to starch biosynthesis.

  7. Screening for sweet potato (Ipomoea batatas (L.)) viruses and their elimination using thermotheraphy-meristem tip culture technique

    International Nuclear Information System (INIS)

    Arkorful, E.

    2012-11-01

    Despite its high potential for food security, production of sweet potato is constrained by viruses which reduce yield by 90%. It is therefore essential to screen for, identify and eliminate these viruses in elite clones before dissemination to farmers. In this study, visual symptomatology and PCR-based techniques were used to identify sweet potato viruses. Visual symptomatology revealed virus associated symptoms ranging from vein clearing, interveinal chlorosis, chlorotic spots, upward curling on leaf edges, leaf narrowing and distortion, purpling, blistering, reduction of the leaf blades and general leaf yellowing in all 22 accessions grown on the field. Disease Incidence (DI) significantly (p≤0.05) varied between accessions with US003 having the lowest (20%) while ten accessions had 90% DI at the end of the study. Index of symptom severity of all plants (ISSap) ranged from 1.08±0.09 to 3.67 ±0.11 with VOTCR003 having the lowest suggesting that it is a moderately susceptible accession while VOTCR002 had the highest suggesting that it is susceptible to viral diseases. Contrarily, index of symptom severity of diseased plants (ISSdp) ranged from 2.00±0.25 to 3.75±0.32. The accession VOTCR002 had the highest ISSdp. Visual symptomatology showed that VOTCR002 had the highest DI, ISSap and ISSdp suggesting that it is highly susceptible to viral diseases. Ten severely infected accessions were tested for Sweet Potato leaf curl virus (SPLCV), Sweet Potato chlorotic stunt virus (SPCSV), Sweet potato feathery mottle virus (SPFMV) and Sweet Potato mild mottle virus (SPMMV) using PCR and RT-PCR techniques. RT-PCR did not amplify any of the virus genomes due to prolonged storage enzymes, In contrast, PCR detected SPLCV in 30% of the accessions. Plants infected with SPLCV were grown in the chamber at 35 degrees celsius for 4 weeks followed by meristem top culture. The regenerants were indexed after ten weeks for SPLCV. Fifty two percent (52.385 od the regenerants were successfully cleaned of the virus and these are being multiplied for farmers. Virus indexing will enhance the dissemination of disease-free plants to farmers. [au

  8. Differences in morphology and sugar content of purple sweet potato (Ipomoea batatas L.) with potassium treatment at several altitudes

    Science.gov (United States)

    Sulistiani, R.; Rosmayati; Siregar, L. A. M.; Harahap, F.

    2018-02-01

    This research was conducted at three locations in low, medium and high plains. This study was aimed to determine the morphological changes and sugar content of sweet potato caused by potassium dose treatment and climate change. Data was analyzed by factorial randomized block design in time series with two factors. The first factor was Altitude: A1 (50 meter above sea level (MASL)), A2 (750 MASL) and A3 (1450 MASL). The second factor was Potassium: K0 (0 kg/ha), K1 (50 kg/ha), K2 (100 kg/ha) and K3 (150 kg/ha). The data of plant morphology change and sugar content was descriptively analized, while agronomic and harvest component data analysis by F test and continued with Duncan Multiple Range Test. The results of morphological observations showed different types of plant growth, which in the lowlands and medium plant growth types spread, twisted, more branches, wider leaf area. However, the plateau of plant growth was relatively dwarf, erect, no twist, huddled and short rods and small leaf area. The tuber weight per plant, number of tuber yield and tuber weight per plot in high altitude were significantly higher than lowland and medium. Similarly, increased altitude will be increased the sugar content significantly.

  9. Altered Phenylpropanoid Metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development

    Science.gov (United States)

    Wang, Hongxia; Yang, Jun; Zhang, Min; Fan, Weijuan; Firon, Nurit; Pattanaik, Sitakanta; Yuan, Ling; Zhang, Peng

    2016-01-01

    There is no direct evidence of the effect of lignin metabolism on early storage root development in sweet potato. In this study, we found that heterologous expression of the maize leaf color (Lc) gene in sweet potato increased anthocyanin pigment accumulation in the whole plant and resulted in reduced size with an increased length/width ratio, low yield and less starch content in the early storage roots. RT-PCR analysis revealed dramatic up-regulation of the genes involved in the lignin biosynthesis pathway in developing storage roots, leading to greater lignin content in the Lc transgenic lines, compared to the wild type. This was also evidenced by the enhanced lignification of vascular cells in the early storage roots. Furthermore, increased expression of the β-amylase gene in leaves and storage roots also accelerated starch degradation and increased the sugar use efficiency, providing more energy and carbohydrate sources for lignin biosynthesis in the Lc transgenic sweet potato. Lesser starch accumulation was observed in the developing storage roots at the initiation stage in the Lc plants. Our study provides experimental evidence of the basic carbohydrate metabolism underlying the development of storage roots, which is the transformation of lignin biosynthesis to starch biosynthesis. PMID:26727353

  10. CLEANER PRODUCTION OF ETHANOL FUEL IPOMOEA BATATA (POTATO) AND ITS IMPACT ON ACCOUNTING RESERVE ENERGY AND SUSTAINABLE DEVELOPMENT OF PERU

    OpenAIRE

    Anaya M., Fernando; Universidad Nacional Mayor de San Marcos, Lima, Perú; Gómez H., Leonel A.; Universidad Nacional Mayor de San Marcos, Lima, Perú; Guerrero A., Marco; Universidad Nacional Mayor de San Marcos, Lima, Perú; Porlles L., José; Universidad Nacional Mayor de San Marcos, Lima, Perú; Woolcott H., Juan; Universidad Nacional Mayor de San Marcos, Lima, Perú; Erazo E., Raymundo; Universidad Nacional Mayor de San Marcos, Lima, Perú

    2014-01-01

    The goal of this research work is the design of a cleaner process for the production of fuel ethanol sweet potato. Applying the principles of conceptual design, Pinch analysis and the use of simulators chem cad and pinch cad, it is determined that the process with molecular sieves is the most effective to get ethanol from 99.5% purity with a power demand of 1400 Kj/kg of ethanol. We conclude that these results impacting positively on the economy of Peru by establishing a path of sustainable d...

  11. Effect of gamma radiation on different explants of sweet potato (Ipomoea batatas L. (Lam)) to induce NaCl tolerance

    International Nuclear Information System (INIS)

    Gutierrez-Rosati, A.

    1997-01-01

    Clonal lines tolerant to NaCl were obtained by combining in vitro culture and gamma radiation in two Peruvian varieties 'Amarillo de Quillabamba' and 'Nemanete'. The most suitable explants were pedicel sections and leaf blades. Embryogenic callus was induced on basal MS basal medium containing 0.5 ppm 2,4-D. The embryogenic calli were irradiated with 5 Gy from a 137 Cs source. Several putative mutants appeared to be stable. (author). 9 refs, 3 tabs

  12. NUTRITIVE VALUE OF FRESH, DRIED (HAY AND ENSILED VINES OF FOUR SWEET POTATO (Ipomoea batatas VARIETIES GROWN IN SOUTHERN ETHIOPIA

    Directory of Open Access Journals (Sweden)

    Gebreegziabher Zereu Hadgu

    2015-08-01

    Full Text Available Chemical composition and in vitro true dry matter digestibility (IVTDMD of fresh, dried (hay and ensiled vines of two early maturing (Belela and Temesgen and two medium maturing varieties (Beletech and Tulla were investigated. Hay was made by sun drying for 3 days. Silages were S1: vine alone, S2: vine+2%molasses, S3: vine+4% molasses, S4: vine+10% sweet potato root (SPR, % of wilted vine weight, S5: vine+20%SPR and S6: vine+30%SPR. They were ensiled in plastic buckets (8 liter with airtight press cape in triplicate for 90 days in a lab at room temperature of 220C. Data were analyzed using SPSS version 20. Hay of Belela, Temesgen, Beletech and Tulla had 17.5, 13.9 and 15.2 %CP (%DM; 36.3, 35.6, 40.5 and 38.8% NDF (%DM and 81.1, 82.2, 75.0 and 76.7% IVTDMD, respectively, were found. There was a  decrease in pH of silages (p<0.05, with increasing levels of both molasses and SPR, respectively (3.91-3.66 and 3.91-3.64 in Belela; 3.92-3.72 and 3.92-3.68 in Temesgen; 3.99-3.81 and 3.99-3.75 in Beletech; 4.02-3.92 and 4.02-3.74 in Tulla; CP (19.8-15.8 and 9.8-13.9 in Belela;  20.0-14.2 and 20.0-12.3 in Temesgen; 17.2-11.2 and 17.2-10.9 in Beletech; 17.9-14.3 and17.9-11.6 inTulla; NDF (29.9-27.7 and 29.9-22.9 in Belela; 26.4-25.0 and  26.4.0-20.8 in Temesgen;36.1-29.3 and 36.1-25.4 in Beletech; 34.5-29.0 and 34.5-24.4 in Tulla; ADF (26.7-18.2 and 26.7-15.5 in Belela; 25.5-17.1 and 25.5-14.7 in Temesgen;30.0-20.2 and 30.0-16.0 in Beletech;29.3-19.1 and 29.3-16.2 in Tulla; and ADL (7.1-4.6 and 7.1-3.5 in Belela; 6.9-4.1 and 6.9-2.7 in Temesgen;9.1- 5.1 and 9.1-4.9 in Beletech; 7.9-5.0 and 7.9-4.1 in Tulla but increased IVTDMD ( 82.5-85.7 and 82.5-87.4, in Belela; 82.1-86.1 and 82.1-88.2 in Temesgen; 79.1-84.1 and 79.1-86.1 in Beletech; 78.9-84.0 and 78.9-85.6 in Tulla. Silages at all additive levels had acceptable pH and good IVTDMD, but 4% molasses, 20 and 30% SPR gave higher IVTDMD. Hay had lower nutrient content and IVTDMD than fresh vine and silages. In conclusion, Belela and Temesgen had better nutritive value than Beletech and Tulla; silage making was better than hay making. Animal evaluation is recommended for further confirmation of the results. Key words: Chemical composition; In vitro true dry matter digestibility; Sweet potato varieties   Key words: Chemical composition; In vitro true dry matter digestibility; Sweet potato varieties

  13. Orange-fleshed sweet potato (Ipomoea batatas) composite bread as a significant source of dietary vitamin A.

    Science.gov (United States)

    Awuni, Victoria; Alhassan, Martha Wunnam; Amagloh, Francis Kweku

    2018-01-01

    Refining food recipes with orange-fleshed sweet potato (OFSP) has the potential to improve dietary intake of vitamin A. The objectives of this study were to utilize OFSP in the development of two composite bread types and to assess their contribution to dietary intake of vitamin A using the dietary reference intake of lactating mothers. Two composite OFSP-wheat flour bread recipes-vita butter bread and vita tea bread-were developed by incorporating 46% OFSP puree in existing 100% wheat flour bread recipes consumed by Ghanaians. A paired-preference test was used to profile the appearance, aroma, sweetness, and overall degree of liking of the vita butter bread and vita tea bread and their respective 100% wheat flour bread types. Weighed bread intake by lactating mothers ( n  =   50) was used to estimate the contribution to dietary vitamin A based on the trans β-carotene content. The developed vita butter bread and vita tea bread were most preferred by at least 77% ( p  <   .05) of consumers ( n  =   310) for all the attributes considered. The average daily intake by the lactating mothers for vita butter bread was 247 g, and for vita tea bread was 196 g. The trans β-carotene content of vita butter bread and vita tea bread were found to be 1.333 mg/100 g and 0.985 mg/100 g, respectively. The estimated trans-β-carotene intake was 3,293 μg/day (vita butter) and 1,931 μg/day (vita tea) based on the weighed bread intake, respectively, meeting 21% and 12% of the daily requirement (1,300 μg RAE/day) for lactating mothers, the life stage group with the highest vitamin A requirement. OFSP therefore could composite wheat flour to bake butter and tea bread, and will contribute to significant amount of dietary intake of vitamin A.

  14. Sweet potato [Ipomoea batatas (L.) Lam.] cultivated as tuber or leafy vegetable supplier as affected by elevated tropospheric ozone.

    Science.gov (United States)

    Keutgen, Norbert; Keutgen, Anna J; Janssens, Marc J J

    2008-08-13

    Sweet potato cultivars respond differently to elevated tropospheric ozone concentrations of ca. 130 mug m (-3), 8 h a day for 4 weeks, which affects their selection for cultivation. In the first cultivar presented here, an adequate leafy vegetable supplier, the ozone load resulted in a shift of biomass to maintain the canopy at the expense of tuber development. Starch content of leaves was reduced, indicating an impairment of quality, but carotenoid content remained stable. The second cultivar may be grown for tuber production. Although the ratio tuber/plant remained stable under ozone, tuber yield and its starch content were significantly reduced. The lower starch content indicated a worse quality for certain industrial processing, but it is desirable for chip production. Elevated tropospheric ozone concentrations also influenced free amino acids and macronutrient contents of tubers, but these modifications were of minor significance for tuber quality in the second cultivar.

  15. Characterization and hepatoprotective activity of anthocyanins from purple sweet potato (Ipomoea batatas L. cultivar Eshu No. 8).

    Science.gov (United States)

    Wang, Lin; Zhao, Ying; Zhou, Qing; Luo, Chun-Li; Deng, Ai-Ping; Zhang, Zi-Cheng; Zhang, Jiu-Liang

    2017-07-01

    The hepatoprotective activity of anthocyanin-rich purple sweet potato extract (APSPE) was demonstrated. Sixty mice were randomly divided into six groups: control group [without carbon tetrachloride (CCl 4 ) or APSPE]; model group (with CCl 4 only); positive control group (50 mg/kg body weight silymarin); low-dose group (100 mg/kg body weight APSPE); medium-dose group (200 mg/kg body weight APSPE); and high-dose group (400 mg/kg body weight APSPE). After 10 days intragastric administration of the respective supplements, the mice in all groups except control were injected intraperitoneally with CCl 4 (0.15% in arachis oil, 10 mL/kg body weight, intravenous). Twelve hours after CCl 4 injection, the mice were measured in terms of liver index, levels of aspartate aminotransferase and alanine aminotransferase in serum, as well as glutathione, superoxide dismutase, and malondialdehyde in liver homogenate. Additionally, the livers of mice were stained with hematoxylin and eosin and sectioned for observation. Nineteen purple sweet potato anthocyanins were identified from the purple sweet potato cultivar Eshu No. 8 and analyzed by liquid chromatography- electrospray ionization-tandem mass spectrometry. Peonidin 3-coumaryl-p-hydroxybenzoyl sophoroside-5-glucoside was first identified in purple sweet potato. The results showed that anthocyanins in Eshu No. 8 had good hepatoprotective activity. Copyright © 2016. Published by Elsevier B.V.

  16. Rapid quantitative determination of maltose and total sugars in sweet potato (Ipomoea batatas L. [Lam.]) varieties using HPTLC.

    Science.gov (United States)

    Lebot, Vincent

    2017-03-01

    When a raw sweet potato root is analysed, only sucrose, glucose and fructose are present but during cooking, starch is hydrolysed into maltose giving the sweet flavour to cooked roots. This study aimed at developing an HPTLC protocol for the rapid quantitative determination of maltose and total sugars in four commercial varieties and to compare them to 243 hybrids grouped by flesh colour (white, orange, purple). In commercial varieties, mean maltose content varied from 10.26 to 15.60% and total sugars from 17.83 to 27.77% on fresh weight basis. Hybrids showed significant variation in maltose content within each group, with means ranging from 7.65% for white-fleshed, to 8.53% in orange- and 11.98% in purple-fleshed. Total mean sugars content was 20.24, 22.11 and 26.84% respectively for white, orange and purple flesh hybrids. No significant correlations were detected between individual sugars but maltose and total sugars content were highly correlated. Compared to the best commercial variety ( Baby ), 25 hybrids (10.3%) presented a higher maltose content and 40 (16.5%) showed a higher total sugars content. HPTLC was observed as an attractive, cost efficient, high-throughput technique for quantitating maltose and total sugars in sweet potatoes. Perspectives for improving sweet potato quality for consumers' requirements are also discussed.

  17. Modeling of mass transfer performance of hot-air drying of sweet potato (Ipomoea Batatas L. slices

    Directory of Open Access Journals (Sweden)

    Zhu Aishi

    2014-01-01

    Full Text Available In order to investigate the transfer characteristics of the sweet potato drying process, a laboratory convective hot air dryer was applied to study the influences of drying temperature, hot air velocity and thickness of sweet potato slice on the drying process. The experimental data of moisture ratio of sweet potato slices were used to fit the mathematical models, and the effective diffusion coefficients were calculated. The result showed that temperature, velocity and thickness influenced the drying process significantly. The Logarithmic model showed the best fit to experimental drying data for temperature and the Wang and Singh model were found to be the most satisfactory for velocity and thickness. It was also found that, with the increase of temperature from 60 to 80°C, the effective moisture diffusion coefficient varied from 2.962×10-10 to 4.694×10-10 m2×s-1, and it fitted the Arrhenius equation, the activation energy was 23.29 kJ×mol-1; with the increase of hot air velocity from 0.423 to 1.120 m×s-1, the values of effective moisture diffusion coefficient varied from 2.877×10-10 to 3.760×10-10 m2•s-1; with the increase of thickness of sweet potato slice from 0.002 m to 0.004 m, the values of effective moisture diffusion coefficient varied from 3.887×10-10 to 1.225×10-9 m2•s-1.

  18. Effect of processing techniques on color and active components amount of sweet potato (Ipomoea Batatas l) flakes

    Science.gov (United States)

    Rahmawati, Y.; Mahmudatussa'adah, A.; Yogha, S.

    2016-04-01

    Sweet potato processing is limited, such as flour, snacks, cystic, or chips. Flakes as pre-cooked meals are made through the stages of making pasta and drying. The purpose of this study was to optimize the production of sweet potato flakes at the stage of making pasta and drying. Making the pasta is done through techniques steamed or baked. Pasta drying using tools a drum dryer or cabinet dryer. As an indicator of optimization is the total of monomeric anthocyanins, β-carotene and color the resulting flakes. The results showed that the amount of anthocyanin monomeric flakes by using steam, and drum dryer (3.83 ± 0.03 mg CYE/g db), flakes by the technique of steam, and cabinet dryer (3.03 ± 0.02 mg CYE/g db), flakes with techniques bake, drum dryer (2.49 ± 0.05 CYE mg/g db), flakes with bake technique, cabinet dryer (1.98 ± 0.03 mg CYE/g db). The Color of purple sweet potato flakes produced through steamed techniques bright purple, while the color purple sweet potato flakes produced through techniques roast give a brownish purple color. The amount of β-carotene yellow flakes sweet potato with stages of cooking steamed, drum dryer (152±0.5 mg/Kg db), grilled drum dryer (136±0.4 mg/Kg db), flakes of yellow sweet potato with stages of roasted and cabinet dryer (140±0.8 mg/Kg db), and grilled stage with cabinet dryer (122±0.3 mg/Kg db). In conclusion sweet potato flakes production techniques through the stages of steam process, and used drum dryers have a number of anthocyanins or β-carotene bigger and brighter colors than the baked flakes techniques and used cabinet dryer.

  19. Diversitas dan sebaran kumbang staphylinid di lahan pertanaman padi (Oryza sativa L. dan ubi jalar (Ipomoea batatas L.

    Directory of Open Access Journals (Sweden)

    Tri Atmowidi

    2017-03-01

    Full Text Available Staphylinid beetle (Coleoptera is characterized by short elytra with orange, brown, or black body color. The objective of this research was to analyse diversity of staphylinid beetles in paddy and sweet potato fields and distribution of the species in sweet potato field. Collections of beetle were done by using road sampling method in two types of agricultural fields. Distribution of beetle population were observed in the plots (1 m x 1 m placed systematically. Seven species of staphylinids were found in this research. Five species (Paederus fuscipes Curtis, Stenus sp., Astenus sp., Hypostenus sp., and Cryptobium abdominale Motsch. were found in paddy fields. We also found five species of staphylinid beetles (P. fuscipes, Medon sp., Stenus sp., Hypostenus prahoeensis Benick, and Philonthus sp. in sweet potato fileds. In paddy and sweet potato fileds were dominated by P. fuscipes. Diversity of staphylinid in paddy and sweet potato fields were low. In sweet potato fields, P. fuscipes showed highest important value index (115.98%, followed by Stenus sp. (69.35%, and H. prahoeensis (5.65%. and Population distribution of P. fuscipes, Medon sp., Stenus sp., dan Philonthus sp. were uniform, while H. prahoeensis was clump.

  20. Biological degradation and composition of inedible sweetpotato biomass

    Science.gov (United States)

    Trotman, A. A.; Almazan, A. M.; Alexander, A. D.; Loretan, P. A.; Zhou, X.; Lu, J. Y.

    1996-01-01

    Many challenges are presented by biological degradation in a bioregenerative Controlled Ecological Life Support System (CELSS) as envisioned by the U.S. National Aeronautics and Space Administration (NASA). In the studies conducted with biodegradative microorganism indigenous to sweetpotato fields, it was determined that a particle size of 75 microns and incubation temperature of 30 degrees C were optimal for degradation. The composition of the inedible biomass and characterization of plant nutrient solution indicated the presence of potential energy sources to drive microbial transformations of plant waste. Selected indigenous soil isolates with ligno-cellulolytic or sulfate-reducing ability were utilized in biological studies and demonstrated diversity in ability to reduce sulfate in solution and to utilize alternative carbon sources: a lignin analog--4-hydroxy, 3-methoxy cinnamic acid, cellulose, arabinose, glucose, sucrose, mannitol, galactose, ascorbic acid.

  1. Soil-to-Plant Transfer of Radiocesium in Ipomoea aquatica

    International Nuclear Information System (INIS)

    Salmah Moosa; Anis Nadia Mohd Faisol Mahadeven; Mohd Noor Hidayat Adenan

    2016-01-01

    The effectiveness of the bio fertilizer regarding the growth promotion and radiocaesium ( 137 Cs) uptake was evaluated in Ipomoea aquatica. The growth of Ipomoea aquatica was enhanced with the application of bio fertilizer. The practice of bio fertilizer resulted in significant increase of 137 Cs concentration in all plant parts and higher 137 Cs transfer from soil to plants. TF values of 137 Cs ranged from 0.555 to 6.726 for the species. The plant roots possessed the lightest weight after being harvest, and show a highest 137 Cs concentration in plant roots. Ipomoea aquatica could be utilized for agricultural countermeasures and phyto remediation, to diminish the allocation of radiocaesium from soil to human. (author)

  2. Arthropod prey of imported fire ants (Hymenoptera: Formicidae) in Mississippi sweetpotato fields.

    Science.gov (United States)

    Rashid, Tahir; Chen, Jian; Vogt, James T; McLeod, Paul J

    2013-08-01

    The red imported fire ants, Solenopsis invicta (Buren), are generally considered pests. They have also been viewed as beneficial predators feeding on other insect pests of various agroecosystems. This study documents the foraging habits of fire ants in a sweetpotato field in Mississippi. Fire ant foraging trails connecting outside colonies to a sweetpotato field were exposed and foraging ants moving out of the field toward the direction of the colony were collected along with the solid food particles they were carrying. The food material was classified as arthropod or plant in origin. The arthropod particles were identified to orders. Fire ant foragers carried more arthropods than plant material. Coleoptera and Homoptera were the most abundant groups preyed upon. These insect orders contain various economically important pests of sweetpotato. Other major hexapod groups included the orders Hemiptera, Diptera and Collembola. The quantity of foraged material varied over the season. No damage to sweetpotato roots could be attributed to fire ant feeding. Imported fire ant foraging may reduce the number of insect pests in sweetpotato fields. © 2012 Institute of Zoology, Chinese Academy of Sciences.

  3. Resin glycosides from Ipomoea pes-caprae.

    Science.gov (United States)

    Escobedo-Martínez, Carolina; Pereda-Miranda, Rogelio

    2007-06-01

    Ipomoea pes-caprae (beach morning-glory; "riñonina" for the herbal drug in Mexico) is prescribed by traditional healers to moderate "heat" in an infected kidney. The hexane-soluble extract from the aerial parts of this medicinal plant, through preparative-scale recycling HPLC, yielded six new lipophilic oligosaccharides of jalapinolic acid: pescaproside B (1) and pescapreins V-IX (2-6). The previously known pescaproside A (7), pescapreins I-IV (8-11), and stoloniferin III (12) were also identified in the analyzed material by means of HPLC comparison with authentic samples. The glycosidic acid structure for all pentasaccharides was confirmed as simonic acid B. Pescaproside B (1), an acylated glycosidic acid methyl ester, is structurally related to pescaprein III (10). Pescapreins V (2) and VI (3) are diasteroisomeric tetraglycosidic lactones of operculinic acid C. Both of these compounds contain (2S)-methylbutyric and n-dodecanoic acids as their esterifying residues. Pescapreins VII (4) and IX (6) are pentasaccharides that contain an n-decanoyl group as their esterifying fatty acid residue instead of the n-dodecanoyl found in pescapreins I (8) and IV (11). Pescaprein VIII (5) represents an isomer of pescaprein II (9) containing an n-dodecanoyl unit as the esterifying residue at position C-4 of the third rhamnose moiety and a 2-methylpropanoyl at C-2 of the second rhamnose. High-field NMR spectroscopy and FAB mass spectrometry were used to characterize all new isolated compounds.

  4. Experimental swainsonine poisoning in goats ingesting Ipomoea sericophylla and Ipomoea riedelii (Convolvulaceae Intoxicação experimental por swainsonina em caprinos ingerindo Ipomoea sericophylla e Ipomoea riedelii

    Directory of Open Access Journals (Sweden)

    Rossemberg C. Barbosa

    2007-10-01

    Full Text Available Ipomoea sericophylla and Ipomoea riedelii cause a glycoprotein storage disease in goats. This paper reports the experimental poisoning in goats by dried I. sericophylla and I. riedelii containing 0.05% and 0.01% swainsonine, respectively. Three groups with four animals each were used. Group 1 received daily doses of 2g/kg body weight (bw of dried I. sericophylla (150mg of swainsonine/kg. Goats from this group had clinical signs 36-38 days after the start of ingestion. Group 2 received dried I. riedelii daily doses of 2g/kg of I. riedelii (30mg of swainsonine/kg for 70 days. No clinical signs were observed, therefore the swainsonine dose was increased to 60mg/kg for another 70 days. Goats from Group 2 had clinical signs 26-65 days after increase in swainsonine dose to 60mg/kg. Group 3 was used as control. In these experiments the minimum toxic dose was 60mg/kg which represents 0.0004% of the dry matter in goats ingesting 1.5% bw of the dry matter. For goats ingesting 2%-2.5% bw of dry matter this dose would be 0.00024%-0.0003% of the dry matter. After the end of the experiment two goats were euthanized and another six were observed for recovery of clinical signs. Four goats that continued to consume swainsonine containing plant for 39-89 days after the first clinical signs had non reversible signs, while two goats that ingested the plant for only 15 and 20 days after the first clinical signs recovered completely. These and previous results indicate that irreversible lesions due to neuronal loss occur in goats that continue to ingest the plants for about 30 days after the first clinical signs. Clinical signs and histological lesions were similar to those reported previously for goats poisoned by swainsonine containing plants. No significant alterations were found in packed cell volume, red and white blood cell counts, hemoglobin and mean corpuscular hemoglobin concentrations, mean corpuscular volume, and serum levels of glucose, total protein, and

  5. Variabilidade morfológica da coleção de germoplasma de batata-doce da Embrapa-Centro Nacional de Pesquisa de Hortaliças

    Directory of Open Access Journals (Sweden)

    Ritschel Patrícia Silva

    2002-01-01

    Full Text Available A coleção de germoplasma de batata-doce (Ipomoea batatas L., mantida pela Embrapa-Centro Nacional de Pesquisa de Hortaliças, foi reunida por meio de expedições de coleta e pela duplicação da coleção anteriormente mantida pela Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura. O objetivo deste trabalho foi descrever e analisar a variabilidade morfológica mantida nessa coleção. Foram avaliados 324 acessos nativos de batata-doce, utilizando-se 25 características morfológicas. Foi possível identificar 256 tipos morfológicos, sendo 223 acessos com morfologia única e 33 grupos de acessos morfologicamente duplicados. Cerca de 20% da coleção consistia de duplicações. Com base na estimativa da diversidade fenotípica mantida na coleção de cada descritor utilizado, considerou-se que a coleção apresenta um nível de polimorfismo alto. Os resultados obtidos foram discutidos em termos de sua utilização na organização da coleção, no planejamento da coleção in vitro dos campos de produção de sementes botânicas e das atividades de caracterização molecular, e no gerenciamento deste recurso genético em âmbito nacional.

  6. Nutrient composition, functional, and pasting properties of unripe cooking banana, pigeon pea, and sweetpotato flour blends.

    Science.gov (United States)

    Ohizua, Ehimen R; Adeola, Abiodun A; Idowu, Micheal A; Sobukola, Olajide P; Afolabi, T Adeniyi; Ishola, Raphael O; Ayansina, Simeon O; Oyekale, Tolulope O; Falomo, Ayorinde

    2017-05-01

    This study investigated some quality attributes of unripe cooking banana (UBF), pigeon pea (PPF), and sweetpotato (SPF) flour blends. Simplex centroid mixture design was used to obtain 17 blends from the flours. The nutrient composition, color, and functional properties of the blends were evaluated using standard methods. Data were subjected to analysis of variance and treatment means were compared using Duncan's multiple range test at 5% probability level. There were significant ( p  pigeon pea-sweetpotato flour blends are desirable for alleviating malnutrition in Nigeria and developing new food formulations.

  7. An Overview of Ipomoea carnea subspecies fistulosa toxicosis in ...

    African Journals Online (AJOL)

    Ipomoea carnea subsp. fistulosa (convolvulaceae) is identified and confirmed as a poisonous plant to animals in many parts of the world. It is evergreen and common in Sahel region of Nigeria where there is lack of green pasture in most part of the year. It contains two toxic principles, swainsonine and calystegines causing ...

  8. Impact of the invader Ipomoea hildebrandtii on grass biomass ...

    African Journals Online (AJOL)

    The invasive coloniser Ipomoea hildebrandtii aggravates the problem of inadequate grass forage in Kajiado district, Kenya. To test its impact on grass biomass, nitrogen (N) mineralisation and seedling establishment, grazing and coloniser density were controlled using experimental exclosures and weeding treatments ...

  9. Recovery of methane-rich gas from solid-feed anaerobic digestion of ipomoea (Ipomoea carnea).

    Science.gov (United States)

    Sankar Ganesh, P; Sanjeevi, R; Gajalakshmi, S; Ramasamy, E V; Abbasi, S A

    2008-03-01

    Studies are presented on new types of anaerobic digesters in which chopped or dry crushed Ipomoea carnea was fed without any other pretreatment, in an attempt to develop commercially viable means of utilizing the otherwise very harmful plant. Two types of solid-feed anaerobic digesters (SFADs) were studied. The first type had a single vessel in which the bottom 35% portion was separated from the top portion by a perforated PVC disk. The weed was charged from the top and inoculated with anaerobically digested cowdung-water slurry. The fermentation of the weed in the reactor led to the formation of volatile fatty acids (VFAs) plus some biogas. The leachate, rich in the VFAs, was passed through the perforated PVC sheet and collected in the lower portion of the vessel. The other type of reactors had two vessels, the first one was fully charged with the weed and the second received the VFA leachate. With both types were attached upflow anaerobic filters (UAFs) which converted the leachate into combustible biogas consisting of approximately 70% methane. All SFADs developed very consistent performance in terms of biogas yield within 17 weeks of start. The two-compartment reactors yielded significantly more biogas than the single-compartment reactors of corresponding total volume, and the reactors with which anaerobic filters (AF) were attached yielded more biogas than the ones without AF. The best performing units generated 2.41m(3) of biogas per m(3) of digester volume, as compared to 0.1-0.2m(3) of biogas, m(-3)d(-1), obtainable with conventional digesters. This indicates the viability of this technology. The spent weed can be vermicomposted directly to obtain good soil-conditioner cum fertilizer; earthworm Eudrilus eugeniae produced 540mg vermicast per animal every day, achieving near total conversion of feed to vermicast in 20 days. The proposed systems, thus, makes it possible to accomplish total utilization of ipomoea.

  10. Translocation of 14C-photosynthates from the leaves at different stages of development in Ipomoea grafts

    International Nuclear Information System (INIS)

    Kato, Shinjiro; Hozyo, Yoshio; Shimotsubo, Kunji

    1979-01-01

    The effects of a sink on the translocation process of 14 C-photosynthesized products from the leaves of several leaf plastochron indices (LPI) at different stages of development in Ipomoea grafts were examined. Grafts were obtained by grafting the scions of an improved variety (I. batatas Poiret var. Okinawa No. 100) on the stocks of wild type (I. trifida (H.B.K.) Don. T-15) or the stocks of Okinawa No. 100. The leaves of LPI 4, 7, 12 and 19 (LPI 4, leaf area was about 65% of that of mature leaves; LPI 12 and 19, leaves reached full expansion stage) were exposed to 14 CO 2 gas for 30 minutes. The plants were harvested 1 hour after the beginning of exposure to 14 CO 2 gas. When the leaves of LPI 4 were exposed to 14 CO 2 gas, 14 C-photosynthesized products translocated slightly in upward and downward directions. With the leaves of LPI 7, downward translocation of 14 C-photosynthesized products was more predominant in the grafts of Okinawa No. 100 stocks than that in the grafts of T-15 stocks. With LPI 12 leaves, downward translocation was greater than that of LPI 7 in the grafts on T-15 stocks. With the leaves of LPI 19, no difference of the translocation pattern of 14 C-photosynthesized products was found between the grafts, and translocation of 14 C-photosynthesized products was found predominantly in downward direction in both stock grafts. The translocation of 14 C-photosynthesized products from the leaves of LPI 4 seemed to be affected by the stock types, that from the leaves of LPI 7 to be affected strikingly in downward translocation by the stock types, and that from the leaves of LPI 19 to be affected in upward and downward translocation by the stock types. (Kaihara, S.)

  11. Long-term storage of sweetpotato by small-scale farmers through ...

    African Journals Online (AJOL)

    Mo

    Kapinga (Eds.), Sweetpotato post harvest assessment -. Experiences from East Africa, London, NRI, pp. 51-66. Rees, D., van Oirschot, Q. E. A., Mbilinyi, L. B., Muhanna,. M. and Tomlins, K. I., 2003b. Improving long-term storage under tropical conditions: role of cultivar selection. In: D. Rees, Q. van Oirschot and R. Kapinga.

  12. Isolation of endophytic diazotroph Pantoea agglomerans and nondiazotroph Enterobacter asburiae from sweetpotato stem in Japan.

    Science.gov (United States)

    Asis, C A; Adachi, K

    2004-01-01

    To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduction activity (ARA) 5 days after incubation at 30 degrees C. Twelve isolates were obtained from MR plates inoculated with a loop of semi-solid MR medium from ARA+ tubes. However, ARA test showed that only nine isolates were diazotrophic and three were nondiazotrophic strains. Using the API 20E diagnostic kit, four diazotrophic isolates were identified as strains of Pantoea spp. and five isolates as Klebsiella spp. The nondiazotrophic bacteria were strains of Enterobacter spp. A diazotrophic isolate Pantoea sp. MY1 and nondiazotrophic isolate Enterobacter sp. MY2 were identified to the species level by full sequence analysis of 16S rRNA gene. The results showed that MY1 had 99.2% similarity to Pantoea agglomerans ATCC 27155 and MY2 had 99.5% similarity to Enterobacter asburiae ATCC 35953. The stem of sweetpotato cv. Koganesengan was colonized by diazotrophic endophyte P. agglomerans and nondiazotrophic endophyte E. asburiae. This study is an essential step toward understanding the ecology and interaction between endophytic bacteria and sweetpotato.

  13. Arthropod prey of imported fire ants (Hymenopter: formicidae) in Mississippi sweetpotato fields

    Science.gov (United States)

    Imported fire ants, Solenopsis invicta (Buren) are generally considered pests. They have also been viewed as beneficial predators feeding on other insect pests of agricultural and medical importance in various agroecosystems. This study documents the foraging habits of fire ants in sweetpotato. Swee...

  14. exploitation of genetic potential of sweetpotato for end-user traits

    African Journals Online (AJOL)

    USER

    This study was conducted to evaluate the breeding potential of sweetpotato germplasm for the .... study are presented in Table 1. The planting arrangement was one row per ridge, with a distance of 1 m between ridges. The length of a ridge was 3.6 m and within row planting ..... content indicates that it is feasible to develop.

  15. Potencialidade de processamento industrial de cultivares de batatas

    Directory of Open Access Journals (Sweden)

    Émerson Loli Garcia

    2015-10-01

    Full Text Available A industrialização da batata tem sido limitada no Brasil, devido à falta de matéria-prima adequada, sendo a demanda suprida pelas importações de produtos processados. Oito cultivares de batata, adaptadas às condições da região serrana de Minas Gerais, safra 2011, foram avaliadas quanto a sua composição centesimal, cor e conteúdo de amilose. Os resultados obtidos mostraram que as cultivares 'Marcy' e 'Colorado' apresentam características importantes para o cozimento. As cultivares 'Marlen' e 'Pirassu' apresentam atributos requeridos para o processamento na forma de chips e/ou palha e a cultivar 'BRS Ana' para a forma de palitos pré-fritos.

  16. Pests, diseases and crop protection practices in the smallholder sweetpotato production system of the highlands of Papua New Guinea

    OpenAIRE

    Gurr, Geoff M.; Liu, Jian; Johnson, Anne C.; Woruba, Deane N.; Kirchhof, Gunnar; Fujinuma, Ryosuke; Sirabis, William; Jeffery, Yapo; Akkinapally, Ramakrishna

    2016-01-01

    Sweetpotato (Ipomea batatans) is a food crop of global significance. The storage roots and foliage of crop are attacked by a wide range of pests and diseases. Whilst these are generally well controlled in developed countries using approaches such as clean planting material and monitoring with pheromone traps to guide insecticide use, research into methods suitable for developing countries has lagged. In Papua New Guinea (PNG), sweetpotato is grown extensively as a subsistence crop and commerc...

  17. Respiratory activity and browning of minimally processed sweet potatoes Atividade respiratória e escurecimento de raízes de batata-doce minimamente processada

    Directory of Open Access Journals (Sweden)

    Celso L. Moretti

    2002-09-01

    Full Text Available Sweet potatoes (Ipomoea batatas L., 'Brazlândia Roxa', 'Brazlândia Branca' and 'Princesa' were harvested at optimum maturity to evaluate respiratory activity and browning susceptibility of minimally processed roots. After harvest, non-blemished roots were graded for size (18±2cm and diameter (5±1cm, and minimally processed inside a cold room. Processed roots were placed in sealed glass jars and stored at 3±0.5°C to evaluate respiratory activity during a 4-hour period or were packed in plastic films with partial vacuum to evaluate development of browning. Packages were stored under refrigerated conditions (3±0.5°C for 5 days. Daily, minimally processed roots were evaluated for browning according to a scale ranging from 0 (extremely browned to 5 (no browning and using an objective assay (absorbance at 340 nm. Minimally processed roots showed a pronounced increase in carbon dioxide evolution immediately after processing. 'Princesa' had the highest respiratory activity among the evaluated cultivars, being 40% higher two hours after processing than the other cultivars. 'Brazlândia Roxa' and 'Brazlândia Branca' were significantly less susceptible to browning compared to 'Princesa', which was rated as unacceptable for commercial use at the end of the storage period. 'Brazlândia Roxa' and 'Brazlândia Branca' were still marketable at the end of the storage period.Batata doce (Ipomoea batatas L., 'Brazlândia Roxa', 'Brazlândia Branca' e 'Princesa' foram colhidas no ponto ótimo de maturidade hortícola, com o objetivo de avaliar-se a atividade respiratória e a suscetibilidade ao escurecimento de raízes minimamente processadas. Após a colheita, raízes sem danos mecânicos aparentes foram selecionadas para tamanho (18±2cm e diâmetro (5±1cm e foram minimamente processadas no interior de uma câmara fria. As raízes minimamente processadas foram colocadas em frascos de vidro hermeticamente fechados e armazenados a 3±0,5°C para avaliar

  18. UNA NUEVA ESPECIE DE IPOMOEA (CONVOLVULACEAE PARA COLOMBIA

    Directory of Open Access Journals (Sweden)

    JHON INFANTE-BETANCOUR

    2014-12-01

    Full Text Available Se describe e ilustra Ipomoea austinii sp. nov. una especie nueva de la región Caribe colombiana. Esta especie se conoce solamente de bordes de caminos asociados a cultivos de palma de aceite (Elaeis guineensis Jacq. en el sur del departamento del Cesar. Se comentan características morfológicas novedosas para el género, así como las posibles afinidades taxonómicas de esta especie dentro del grupo.

  19. PERKEMBANGAN AERENKIM AKAR KANGKUNG DARAT (Ipomoea reptans Poir DAN KANGKUNG AIR (Ipomoea aquatic Forsk

    Directory of Open Access Journals (Sweden)

    Aliya Ningsih

    2016-09-01

    Full Text Available Abstrak Penelitian tentang perkembangan aerenkim pada kangkung darat dan kangkung air telah dilakukan pada bulan Oktober 2014 sampai Februari 2015 di Laboratorium Struktur Perkembangan Tumbuhan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas. Jaringan akar diproses dengan metode parafin, kemudian dideskripsikan secara kuantitatif. Rongga aerenkim pada kangkung air terbentuk pada minggu ke tiga dengan jumlah dua rongga sel sedangkan pada kangung darat terbentuk pada minggu ke empat. Proses pembentukan aerenkim terjadi melalui proses pelisisan sel korteks.Abstract Research of aerenchyma development on terrestrial kale Ipomea reptans poir and water kale Ipomoea aquatic Forsk was conducted from October, 2014 until February, 2015 at Laboratory of plant growth structure, the Faculty of Math and Science, Department of Biology, Andalas University. Kales were analyzed by using paraffin method then described by quantitive data. Results showed: (i aerenchyma cavities were formed in the 1st week, (ii aquatic Forsks with two cell cavities were formed in the 3rd week, (iii terrestrial kales were formed in the 4th week. The formation process of aerenchyma occurred through lysis process of cortical cell.

  20. Control of amphibious weed ipomoea (Ipomoea carnea by utilizing it for the extraction of volatile fatty acids as energy precursors

    Directory of Open Access Journals (Sweden)

    M. Rafiq Kumar

    2015-01-01

    Full Text Available Volatile fatty acids (VFAs, comprising mainly of acetic acid and lesser quantities of propionic and butyric acids, are generated when zoomass or phytomass is acted upon by acidogenic and acetogenic microorganisms. VFAs can be utilized by methanogens under anaerobic conditions to generate flammable methane–carbon dioxide mixtures known as ‘biogas’. Acting on the premise that this manner of VFA utilization for generating relatively clean energy can be easily accomplished in a controlled fashion in conventional biogas plants as well as higher-rate anaerobic digesters, we have carried out studies aimed to generate VFAs from the pernicious weed ipomoea (Ipomoea carnea. The VFA extraction was accomplished by a simple yet effective technology, appropriate for use even by laypersons. For this acid-phase reactors were set, to which measured quantities of ipomoea leaves were charged along with water inoculated with cow dung. The reactors were stirred intermittently. It was found that VFA production started within hours of the mixing of the reactants and peaked by the 10th or 11th day in all the reactors, effecting a conversion of over 10% of the biomass into VFAs. The reactor performance had good reproducibility and the process appeared easily controllable, frugal and robust.

  1. Polpa de batata residual acidificada desidratada: aplicação em biscoitos tipo rosquinha

    OpenAIRE

    Morais, Mariana Patricio de

    2014-01-01

    O descarte de resíduos provenientes do processamento da batata frita no meio ambiente pode trazer problemas de poluição. A reutilização desses resíduos traz uma redução gradual no custo de produção de produtos de consumo. Este estudo teve como objetivo verificar a viabilidade física, química, tecnológica, microbiológica e sensorial de substituir parcialmente a farinha de trigo por polpa de batata residual acidificada desidratada proveniente da industrialização de batata frita na formulação de...

  2. A plant culture system for producing food and recycling materials with sweetpotato in space

    Science.gov (United States)

    Kitaya, Yoshiaki; Yano, Sachiko; Hirai, Hiroaki

    2016-07-01

    The long term human life support in space is greatly dependent on the amounts of food, atmospheric O2 and clean water produced by plants. Therefore, the bio-regenerative life support system such as space farming with scheduling of crop production, obtaining high yields with a rapid turnover rate, converting atmospheric CO2 to O2 and purifying water should be established with employing suitable plant species and varieties and precisely controlling environmental variables around plants grown at a high density in a limited space. We are developing a sweetpotato culture system for producing tuberous roots as a high-calorie food and fresh edible leaves and stems as a nutritive functional vegetable food in space. In this study, we investigated the ability of food production, CO2 to O2 conversion through photosynthesis, and clean water production through transpiration in the sweetpotato production system. The biomass of edible parts in the whole plant was almost 100%. The proportion of the top (leaves and stems) and tuberous roots was strongly affected by environmental variables even when the total biomass production was mostly the same. The production of biomass and clean water was controllable especially by light, atmospheric CO2 and moisture and gas regimes in the root zone. It was confirmed that sweetpotato can be utilized for the vegetable crop as well as the root crop allowing a little waste and is a promising functional crop for supporting long-duration human activity in space.

  3. Produção de sementes de batata-doce, por fecundações artificiais, em casa de vegetação Seed setting of sweet potato hand pollinated under grenhouse conditions

    Directory of Open Access Journals (Sweden)

    Domingos Antonio Monteiro

    1992-01-01

    Full Text Available No período de 1970-1977, estudou-se a produção de sementes verdadeiras de batata-doce (Ipomoea batatas (L. Lam., por fecundações artificiais em condições de casa de vegetação, cultivando-se segmentos de ramas com botões florais em vasos com água. Os resultados evidenciaram diferença de comportamento dos genótipos quanto à capacidade de produção de sementes. O genótipo SRT 252 (Rama Grossa foi o que melhor se comportou como planta feminina, com formação de 57,40% de cápsulas quando cruzado com outros genótipos e 40,20% quando autofecundado.Seed setting of five sweet potato genotypes: SRT 72-Jacareí; SRT 248; SRT 252-Rama Grossa; IAC 66-118-Monalisa and IAC 3-4-Irani was studied under greenhouse conditions. Flowers from vine cuttings growing in water were hand pollinated. All five lines functioned about equally well as pollen parents but there were significant differences in their ability to function as seed parents. Cultivars SRT 248 and SRT 72 when used as female showed a low ability to seed production both when selfed and crossed. Such ability appeared to be a varietal characteristic which affected seed set. Differences were found between the percentage of seed set of open and cross-pollinated flowers. Self incompatibility was observed for four cultivars. The highest degree of self compatibility was showed by the cultivars SRT 252. There was an observed decline in seed set during the month of June, with the highest seed set during April.

  4. Atividade antiulcerogênica de Ipomoea imperati (Vahl) Griseb (Convolvulaceae)

    OpenAIRE

    Miyahara, Maria Regina Mortatti

    2008-01-01

    Ipomoea imperati (Convolvulaceae) é usada na medicina tradicional para o tratamento da inflamação, inchaço e feridas, bem como para tratar dor pós- parto e para problemas do estômago. I. imperati, apresenta compostos químicos como alcalóides indólicos e resinas glicosídicas, que são os componentes biológicos comumente ativos na família Convolvulaceae. Em estudos anteriores, I. imperati inibiu a inflamação tópica e sistêmica de maneira concentração-dependente, bloqueando significativamente a a...

  5. Pentasaccharide resin glycosides from Ipomoea cairica and their cytotoxic activities.

    Science.gov (United States)

    Yu, Bangwei; Luo, Jianguang; Wang, Junsong; Zhang, Dongming; Yu, Shishan; Kong, Lingyi

    2013-11-01

    Six partially acylated pentasaccharide resin glycosides, cairicosides A-F, were isolated from the aerial parts of Ipomoea cairica. These compounds were characterized as a group of macrolactones of simonic acid A, partially acylated with different organic acids. The lactonization site of 11S-hydroxyhexadecanoic acid (jalapinolic acid) was bound to the second saccharide moiety at C-3 in cairicosides A-E, while at C-2 in cairicoside F. Structures were established by spectroscopic and chemical methods. Compounds cairicosides A-E exhibited moderate cytotoxicity against a small panel of human tumor cell lines with IC50 values in the range of 4.28-14.31μM. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. Genotype Stability Index for Root Yield and Tolerance to Sweetpotato Weevil Cylas puncticolis: A Tool for Identifying Climate Smart Varieties

    Directory of Open Access Journals (Sweden)

    Chalwe A.

    2017-02-01

    Full Text Available Despite the ability of sweetpotato to grow in marginal areas, large differential genotypic responses have been reported under varying environmental conditions. Differences in pest and disease pressure contribute significantly to inconsistencies in performance of genotypes in various environments. Using a randomized complete block design, eight sweetpotato genotypes were evaluated in one location successively for five years (seasons (2010-2015. Additive main effects and multiplicative interaction (AMMI stability value (ASV was used to identify best genotypes that combine stability with high resistance to sweetpotato weevil Cylas puncticolis and root yield across the five seasons (years. Stability of genotypes for weevil infestation and damage thereof, and fresh storage root yield were determined for each season. The data on each of these parameters were correlated with rainfall and temperature data for each and across five seasons. Results show variability in the ranking of genotypes’ stability for resistance to weevil infestation and associated damage. Significant negative correlation was recorded between total rainfall and sweetpotato weevil damage. However, AMMI analysis of variance indicates genotype main effects, environmental main effects and the interaction thereof were all significant for root yield and weevil damage. Genotype selection index assisted to identify at least three genotypes namely Kokota, Lunga, and Kalungwishi which combined stability for high root yield and tolerance to weevil damage.

  7. Manejo da adubação nitrogenada paraa batata-doce: fontes e parcelamento de aplicação Management of nitrogen fertilization for sweet potato: sources and application parceling

    Directory of Open Access Journals (Sweden)

    Anarlete Ursulino Alves

    2009-12-01

    Full Text Available Com o objetivo de avaliar o rendimento da batata-doce [Ipomoea batatas (L. Lam.], cultivar Rainha Branca, submetida a fontes e parcelamentos da aplicação de 80 kg ha-1 de nitrogênio, instalou-se um experimento, no período de abril a agosto de 2007, na Universidade Federal da Paraíba, em Areia. O delineamento experimental utilizado foi o de blocos casualizados, em esquema fatorial 2 x 7, com duas fontes de nitrogênio (uréia e sulfato de amônio e oito de épocas parcelamentos da aplicação (100% no plantio; 100% aos 30 dias após o plantio (DAP; 100% aos 60 DAP; 50% no plantio e 50% aos 30 DAP; 50% no plantio e 50% aos 60 DAP; 50% aos 30 e 50% aos 60 DAP; 33% no plantio, 33% aos 30 e 33% aos 60 DAP, com quatro repetições. Foram avaliados o peso médio de raízes comerciais, a produção e o número de raízes comerciais por planta e as produtividades total e comercial de raízes de batatadoce. O maior peso médio de raízes comerciais (294 g foi obtido com o parcelamento do N, como uréia, 50% no plantio e 50% aos 30 DAP. O parcelamento de N 33% no plantio, 33% aos 30 DAP e 33% aos 60 DAP na forma sulfato de amônio foi responsável pela maior produção de raízes comerciais por planta (337 g. Para o número de raízes comercias e as produtividades total e comercial, o N na forma de sulfato de amônio parcelado 33% no plantio, 33% aos 30 DAP e 33% aos 60 DAP proporcionou os maiores valores, 1,50 raízes, 30,5 e 28,4 t ha-1, respectivamente.For the purpose of evaluating the yield of the sweet potato [Ipomoea batatas (L. Lam.], variety "Rainha Branca", subjected to sources and parceling of nitrogen, an experiment was carried out in soil classified as typical "Inceptisol Regolítico Psamítico" at the Universidade Federal da Paraíba, Areia county, from April to August 2007. The experimental design used was one of randomized blocks, with a factorial scheme of 2 x 7, using two sources of nitrogen fertilizer (urea and ammonium sulfate and

  8. Yield, nutritional quality and stability of orangefleshed sweetpotato cultivars successively later harvesting periods in Mozambique

    Directory of Open Access Journals (Sweden)

    Alvaro Abilio

    2017-08-01

    Full Text Available Long-term storage of sweetpotato roots is a great challenge for smallholder farmers in Mozambique. Piecemeal harvesting allows several months supply of roots for household consumption provided weevil infestation is avoided. The objectives of the present studies were to determine yield and changes in key macro- and micronutrients associated with early or late harvesting of orange-fleshed sweetpotato cultivars in Mozambique. Four trials representing harvesting periods of 3, 4, 5 or 6 months after planting were established at Gurue in 2015. The randomized complete block design with three replications was laid in each trial. Yield measurements were done in the field and samples were selected and scanned for dry matter, beta-carotene, iron, zinc and carbohydrate using Near Infrared Spectrometry. Collected data were statistically analysed (SAS 1997 software. Yield, dry matter, starch, iron and beta-carotene increased linearly in some cultivars as time to harvest was prolonged. Iron was not affected by harvesting period. Stability of micronutrients such as iron, zinc and beta-carotene is essential when piecemeal harvesting. The study allowed accurate grouping of the cultivars tested into maturity groups for the first time.

  9. KARAKTERISTIK FISIKO-KIMIA TEPUNG UBI JALAR (Ipomoea batatas VARIETAS SUKUH DENGAN VARIASI PROSES PENEPUNGAN [Physico-Chemical Characteristics of Sukuh Variety Sweet Potatoes (Ipomea batatas Flours Made with Various Methods

    Directory of Open Access Journals (Sweden)

    Trifena Honestin

    2009-12-01

    Full Text Available Sweet potato has considerable potencies to support food diversification program based on flour and starch product. Various processing methods in the flour processing show great effect on alteration of the physicochemical properties of sweet potato flour. This research investigated the effects of different flour processing methods on the physicochemical properties of sweet potato flour. The results showed that processing method had a significant effect on water content, bulk density, colour (L, a, b, microscopic properties of starch granule, water absorption index, water soluble index and pasting properties of the sweet potato flour.

  10. Genetic differentiation of Slovenian sweet potato varieties (Ipomoea batatas and effect of different growing media on their agronomic and nutritional traits

    Directory of Open Access Journals (Sweden)

    Lovro Sinkovič

    2017-12-01

    Full Text Available The objectives of the present study were firstly to analyse the genetic relatedness among three Slovenian sweet potato varieties; and secondly to assess the effects of different growing media on selected agronomic and nutritional traits of these varieties. Tubers of three varieties (Lučka, Janja, Martina with different skin/flesh colour were produced in planters under glasshouse conditions in five different growing media (perlite, peat, expanded clay, vermiculite, garden soil from prior raised seedlings. Genetic analysis was performed using a set of eight single sequence repeats markers. According to Nei’s genetic distance and pairwise population Fst analysis, the most related varieties are Janja and Martina; in contrast, Lučka and Martina show the weakest genetic relationships. The following agronomic traits were evaluated: vine length, thickness of vine-base, number of branches, weight of above ground part, number of leaves plant–1, number of tubers plant–1 and tubers weight plant–1. Between nutritional traits total phenolic content (TPC, antioxidant potential (AOP and ascorbic acid content (AA were determined in the range: 36.2 to 65.1 mg gallic acid equivalent 100 g–1 fresh weight, 0.18 to 0.56 mg Trolox equivalents g–1 FW and 13.7 to 23.5 mg 100 g–1 FW, respectively. Significant interactions of growing media (factor A × variety (factor B were observed for thickness of vine-base, weight of above ground part, AOP, TPC and AA. Overall, results showed variation in varieties response to growing media. Growing media provide a discriminant classification of the sweet potato varieties according to their agronomic and nutritional traits.

  11. Stability of carotenoids, total phenolics and in vitro antioxidant capacity in the thermal processing of orange-fleshed sweet potato (Ipomoea batatas Lam.) cultivars grown in Brazil.

    Science.gov (United States)

    Donado-Pestana, Carlos M; Mastrodi Salgado, Jocelem; de Oliveira Rios, Alessandro; dos Santos, Priscila Robertina; Jablonski, André

    2012-09-01

    Intervention strategies regarding the biofortification of orange-fleshed sweet potato, which is a rich source of carotenoids for combating vitamin A deficiency, are being developed in Brazil. This study was conducted to evaluate the concentrations of individual carotenoids, total phenolic compounds and antioxidant capacity in the roots of four biofortified sweet potato cultivars that were raw or processed by four common heat treatments. HPLC, Folin-Ciocalteu, DPPH and ABTS assays were used. All cultivars showed high levels of carotenoids in raw roots, predominantly all-trans-β-carotene (79.1-128.5 mg.100 g(-1) DW), suggesting a high estimated vitamin A activity. The CNPH 1194 cultivar reported carotenoids values highest than those of other cultivars (p < 0.05). The total phenolic compounds varied among cultivars and heat treatments (0.96-2.05 mg.g(-1) DW). In most cases, the heat treatments resulted in a significant decrease in the carotenoids and phenolic compounds contents as well as antioxidant capacity. Processing of flour presented the greatest losses of major carotenoids and phenolics. The phenolic compounds showed more stability than carotenoids after processing. There were significant correlations between the carotenoids and phenolic compounds and the antioxidant capacity.

  12. Effects of anthocyanins from purple sweet potato (Ipomoea batatas L. cultivar Eshu No. 8) on the serum uric acid level and xanthine oxidase activity in hyperuricemic mice.

    Science.gov (United States)

    Zhang, Zi-Cheng; Su, Guan-Hua; Luo, Chun-Li; Pang, Ya-Lu; Wang, Lin; Li, Xing; Wen, Jia-Hao; Zhang, Jiu-Liang

    2015-09-01

    This study was aimed at evaluating the hypouricemic effect of the anthocyanin-rich purple sweet potato extract (APSPE). In vitro, APSPE has been proved to significantly inhibit XO activity in a dose-dependent manner. In vivo, APSPE could not only inhibit the XO activity in mouse liver, but also reduce the serum uric acid level in hyperuricemic mice and affect the expression of mRNA levels of related renal transporters, such as mURAT1, mGLUT9, mOAT1 and mOCTN2. Moreover, APSPE could effectively regulate BUN and Cr levels to normal and decrease the inflammatory cellular influx in the tubule of the hyperuricemic mice. This study indicates the potential clinical utility of APSPE as a safe and effective anti-hyperuricemia bioactive agent or functional food.

  13. Defensin protein from sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage roots exhibits antioxidant activities in vitro and ex vivo.

    Science.gov (United States)

    Huang, Guan-Jhong; Deng, Jeng-Shyan; Chen, Hsien-Jung; Huang, Shyh-Shyun; Liao, Jung-Chun; Hou, Wen-Chi; Lin, Yaw-Huei

    2012-12-01

    This study was designed to investigate the antioxidant activities of sweet potato defensin (SPD1) in vitro and ex vivo. Antioxidant status [2,2'-azinobis[3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assay], scavenging activity against DPPH (1,1-dipheny-2-picrylhydrazyl) radical method, reducing power method, Fe(2+)-chelating ability, FTC (ferric thiocyanate) method, and protection of calf thymus DNA against hydroxyl radical-induced damage were studied in vitro. The ex vivo experiments revealed that SPD1 could decrease the production of intracellular peroxide in HepG2 cells. Four peptides, namely GFR, GPCSR, CFCTKPC and MCESASSK for testing antioxidative activity, were synthesized according to tryptic hydrolysis simulation. In the TEAC assay CFCTKPC performed the best (13.5±0.3μmol TE/g dw), even better than reduced glutathione (7.3±0.2μmol TE/g dw). In the DPPH radical assay (%), [IC(50) (μM) (the concentration required for scavenging 50% activity)] CFCTKPC again had the highest antioxidant activity (IC(50) is 11.3±3.2μM) even better than reduced glutathione (IC(50) is 74.3±2.4μM). In the lipid peroxidation assay, once again CFCTKPC performed the best, with an IC(50) value of 0.5±0.0μM better than reduced glutathione (1.2±0.1μM). These findings mean that cysteine residue is most important in antioxidant activities. It was suggested that SPD1 might contribute its antioxidant activities against hydroxyl and peroxyl radicals. Copyright © 2012 Elsevier Ltd. All rights reserved.

  14. Identification of low-Cd cultivars of sweet potato (Ipomoea batatas (L.) Lam.) after growing on Cd-contaminated soil: uptake and partitioning to the edible roots.

    Science.gov (United States)

    Huang, Baifei; Xin, Junliang; Dai, Hongwen; Zhou, Wenjing; Peng, Lijing

    2015-08-01

    Cadmium (Cd) contamination in agricultural products presents a threat to humans when consumed. Sweet potato is the world's seventh most important food crop. The aims of this study were to screen for low-Cd sweet potato cultivars and clarify the mechanisms of low-Cd accumulation in edible roots. A pot experiment was conducted to investigate the variation of Cd uptake and translocation among 30 sweet potato cultivars grown in contaminated soils with three different Cd concentrations. Cadmium concentrations in edible roots were significantly different among cultivars and were significantly affected by Cd treatment, and the interaction between cultivar and Cd treatment. High-Cd cultivars have higher ratios of edible root/shoot Cd concentration and edible root/feeder root Cd concentration than low-Cd cultivars; however, the ratio of shoot/feeder root Cd concentration seems unrelated to the ability of Cd accumulation in edible roots. Four sweet potato cultivars, Nan88 (No. 10), Xiang20 (No. 12), Ji78-066 (No. 15), and Ji73-427 (No. 16), were identified as low-Cd cultivars. Cadmium translocation from feeder roots to edible roots via the xylem, and from shoots to edible roots via the phloem, controls Cd accumulation in edible roots of sweet potato cultivars.

  15. LEMAK TUBUH DAN PROFIL LIPIDA DARAH ITIK BALI JANTAN YANG DIBERI RANSUM MENGANDUNG DAUN UBI JALAR UNGU (Ipomoea batatas L DISUPLEMENTASI STARBIO DAN PIGNOX (STARPIG

    Directory of Open Access Journals (Sweden)

    NI MADE SUCI SUKMAWATI

    2014-06-01

    Full Text Available Penelitian ini bertujuan untuk mengkaji pengaruh pemberian ransum yang mengandung tepung daun ubi jalar ungu disuplementasi Starbio dan Pignox (Starpig terhadap lemak tubuh dan profil lipida darah itik Bali. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL dengan tiga perlakuan, yaitu : Ransum tanpa daun ubi jalar ungu dan Starpig (A, ransum mengandung 0,5% daun ubi jalar ungu (B, dan ransum mengandung 0,5% daun ubi jalar ungu disuplementasi 0,5% Starpig (C. Setiap perlakuan terdiri dari lima ulangan, dan setiap ulangan menggunakan empat ekor itik Bali jantan umur 16 minggu. Variabel yang diamati, meliputi konsumsi antioksidan, lemak abdominal, lemak karkas, lemak tubuh, dan profil lipida darah. Hasil penelitian menunjukkan bahwa pemberian ransum mengandung daun ubi jalar ungu disuplementasi Starpig (C, nyata (P<0,05 dapat meningkatkan konsumsi antioksidan dan menurunkan lemak karkas, lemak abdominal, lemak tubuh, total kolesterol, HDL, LDL, dan trigliserida dibandingkan dengankontrol (A. Dari hasil penelitian ini dapat disimpulkan bahwa pemberian ransumyang mengandung tepung daun ubi jalar ungu disuplementasi Starpig dapat mengurangi lemak tubuh dan memperbaiki profil lipida darah itik Bali, umur 24 Minggu.

  16. Role of non enzymatic synthesis of indole-3-acetic acid in the Ipomoea batatas L. Lam. (sweet potato) response to gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Lage, C.L.S.; Esquibel, M.A. [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Biofisica. Lab. de Fisiologia Vegetal

    1995-12-01

    Indolacetic acid (IAA) production by tryptophan radiolysis was evaluated after irradiation of a tryptophan solution (1.0 mg/ml) with a 2000 Gy dose of gamma rays followed by High Performance Liquid Chromatography (HPLC) analysis. New absorbance peaks at 280 nm were detected in the irradiated solution, one of them having a migration time similar to that of IAA, indicating a0.14% yield for the conversion reaction. The low yield led us to abandon the hypothesis that non-enzymatic IAA synthesis may account for growth simulation. When the electrophoretic patterns of peroxidases from absorbent roots derived from storage roots treated with IAA (1.0 mg/l) or gamma radiation were compared with control, differences were detected only in the irradiated material. Thus the growth radiation-induced effects do not seem to result from a sudden endogenous IAA increment. (author)

  17. Anthocyanins from purple sweet potato (Ipomoea batatas (L.) Lam.) and their color modulation by the addition of phenolic acids and food-grade phenolic plant extracts.

    Science.gov (United States)

    Gras, Claudia C; Nemetz, Nicole; Carle, Reinhold; Schweiggert, Ralf M

    2017-11-15

    Anthocyanin profiles and contents of three purple sweet potato provenances were investigated by HPLC-DAD-MS n . In contrast to widely uniform profiles, the contents of total (558-2477mg/100gDM) and individual anthocyanins varied widely. Furthermore, quantitative and qualitative effects of intermolecular co-pigmentation were studied by adding chlorogenic and rosmarinic acids, and food-grade phenolic apple and rosemary extracts at various dosages to a diluted purple sweet potato concentrate at pH 0.9, 2.6, 3.6, and 4.6. Addition of co-pigments generally increased pK H estimate -values of anthocyanins from 3.28 (without co-pigments) to up to 4.71, thus substantially broadening the pH range wherein colored forms prevail. The most pronounced hyperchromic shift by up to +50.5% at the absorption maximum was observed at pH 4.6. Simply by blending the co-pigments with purple sweet potato anthocyanins at pH-values ranging from 2.6 to 4.6, purplish-blue, light pink, magenta, brick-red, and intense red hues were accessible as expressed by CIE-L ∗ a ∗ b ∗ color values. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. KAJIAN SIFAT FUNGSIONAL DAN AMILOGRAFI PATI UBI JALAR (Ipomoea batatas L. DENGAN PERLAKUAN SUHU DAN LAMA WAKTU HEAT MOISTURE TREATMENT SEBAGAI BAHAN SEDIAAN PANGAN DARURAT

    Directory of Open Access Journals (Sweden)

    marleen sunyoto

    2017-01-01

    Full Text Available Pemuliaan Tanaman Ubi Jalar yang dilakukan di Lahan Percobaan Universitas Padjadjaran telah menghasilkan klon-klon baru ubi jalar unggulan, salah satunya yaitu Awachy 5. Awachy 5 mengandung pati yang cukup tinggi, yaitu 25,46% sehingga dapat diolah lebih lanjut menjadi tepung pati sebagai bahan baku Emergency Food. Namun pati alami belum dimanfaatkan secara optimal karena sifat fungsional dan sifat amilografi pati yang kurang baik, sehingga diperlukan perlakuan lebih lanjut untuk memperbaiki sifat tersebut. Perlakuan modifikasi fisik dengan Heat Moisture Treatment (HMT dapat memperbaiki sifat fungsional dan amilografi pati. Tujuan penelitian adalah untuk memperoleh suhu dan lama waktu modifikasi HMT yang menghasilkan pati ubi jalar dengan sifat fungsional dan amilografi yang berbeda. Metode penelitian yang digunakan adalah metode percobaan dengan Rancangan Acak Kelompok (RAK yang terdiri dari 5 perlakuan dan 3 ulangan, yaitu tanpa pemanasan (kontrol, pemanasan HMT pada suhu 80oC 4 jam;  80oC 8 jam; 110oC 4 jam; dan 110oC 8 jam. Hasil penelitian menunjukkan bahwa pati ubi jalar dengan pemanasan HMT pada suhu 110oC selama 8 jam merupakan perlakuan terpilih dengan swelling volume 4,205 ml/g, solubility 2,117%, freeze thaw stability 48,655%, kekuatan gel 4,684 gf, derajat putih 76,717%, suhu awal gelatinisasi 83,388oC, viskositas puncak 5063,833 cP, viskositas breakdown 486,500 cP, dan viskositas setback 3596,833 cP. Kata kunci : Awachy 5, modifikasi HMT, pati, sifat fungsional, amilografi

  19. Simulation of transient heat transfer during cooling and heating of whole sweet potato (Ipomoea batatas (L.) Lam.) roots under forced-air conditions

    International Nuclear Information System (INIS)

    Korese, Joseph Kudadam; Sturm, Barbara; Román, Franz; Hensel, Oliver

    2017-01-01

    Highlights: • Heat transfer of whole sweet potato roots under forced-air cooling and heating is investigated. • Experiments were carried out in a cooling and heating chamber. • The cooling and heating rate and time was clearly depended on air velocity and roots size. • Simulated and experimental data on cooling and heating times were compared for validation. • Simulation results quantitatively agreed with experimental results. - Abstract: In this work, we investigated how different air velocity and temperature affect the cooling and heating rate and time of individual sweet potato roots. Additionally, we modified and applied a simulation model which is based on the fundamental solution of the transient equations for estimating the cooling and heating time at the centre of sweet potato roots. The model was adapted to receive input parameters such as thermo-physical properties of whole sweet potato roots as well as the surrounding air properties, and was verified with experimental transient temperature data. The experimental results showed that the temperature at the centre and the under skin of sweet potato roots is almost homogeneous during forced convection cooling and heating. The cooling and heating time was significantly (P < 0.05) affected by high air velocity and sweet potato root size. The simulation results quantitatively agreed with the experimental transient data. This research, thus provides a reliable experimental and theoretical basis for understanding the temperature variations as well as estimating the cooling and heating times in individual sweet potato roots under forced convection cooling and heating. The result from this study could be applied to design and optimize forced-air treatment equipments with improved energy efficiency as well as ensuring safety and the maintenance of sweet potato roots quality.

  20. Chemical composition, nitrogen degradability and in vitro ruminal biological activity of tannins in vines harvested from four tropical sweet potato (Ipomoea batatas L.) varieties.

    Science.gov (United States)

    Ali, R; Mlambo, V; Mangwe, M C; Dlamini, B J

    2016-02-01

    This study investigated the potential of vines from four sweet potato varieties (Tia Nong 57, Tia Nong 66, Ligwalagwala and Kenya) as alternative feed resources for ruminant livestock. The chemical composition [neutral detergent fibre (NDF), acid detergent fibre (ADF), crude protein (CP) and acid detergent insoluble nitrogen (ADIN)], in vitro ruminal nitrogen (N) degradability and in vitro ruminal biological activity of tannins in the vines, harvested at 70 and 110 days after planting (DAP), were determined. Variety and harvesting stage did not (p > 0.05) influence CP and NDF content of the vines. Concentration of CP ranged from 104.9 to 212.2 g/kg DM, while NDF ranged from 439.4 to 529.2 g/kg DM across harvesting stages and varieties. Nitrogen degradability (ND) at 70 and 110 DAP was highest (p < 0.05) in Ligwalagwala (743.1 and 985.0 g/kg DM, respectively). Treatment of vines with tannin-binding polyethylene glycol (PEG) increased (p < 0.05) in vitro ruminal cumulative gas production parameters (a, b and c). The in vitro ruminal biological activity of tannins, as measured by increment in gas production parameters upon PEG inclusion, had a maximum value of 18.2%, suggesting low to moderate antinutritional tannin activity. Ligwalagwala vines, with highly degradable N, would be the best protein supplement to use during the dry season when ruminant animals consume low N basal diets and maintenance is an acceptable production objective. Tia Nong 66 and Kenya varieties, with less degradable N, may be more suitable for use as supplements for high-producing animals such as dairy goats. Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH.

  1. Role of non enzymatic synthesis of indole-3-acetic acid in the Ipomoea batatas L. Lam. (sweet potato) response to gamma radiation

    International Nuclear Information System (INIS)

    Lage, C.L.S.; Esquibel, M.A.

    1995-01-01

    Indolacetic acid (IAA) production by tryptophan radiolysis was evaluated after irradiation of a tryptophan solution (1.0 mg/ml) with a 2000 Gy dose of gamma rays followed by High Performance Liquid Chromatography (HPLC) analysis. New absorbance peaks at 280 nm were detected in the irradiated solution, one of them having a migration time similar to that of IAA, indicating a0.14% yield for the conversion reaction. The low yield led us to abandon the hypothesis that non-enzymatic IAA synthesis may account for growth simulation. When the electrophoretic patterns of peroxidases from absorbent roots derived from storage roots treated with IAA (1.0 mg/l) or gamma radiation were compared with control, differences were detected only in the irradiated material. Thus the growth radiation-induced effects do not seem to result from a sudden endogenous IAA increment. (author)

  2. Water-Deficit Tolerance in Sweet Potato [Ipomoea batatas (L. Lam.] by Foliar Application of Paclobutrazol: Role of Soluble Sugar and Free Proline

    Directory of Open Access Journals (Sweden)

    Suravoot Yooyongwech

    2017-08-01

    Full Text Available The objective of this study was to elevate water deficit tolerance by improving soluble sugar and free proline accumulation, photosynthetic pigment stabilization, photosynthetic abilities, growth performance and storage root yield in sweet potato cv. ‘Tainung 57’ using a foliar application of paclobutrazol (PBZ. The experiment followed a Completely Randomized Block Design with four concentrations of PBZ: 0 (control, 17, 34, and 51 μM before exposure to 47.5% (well irrigation, 32.3% (mild water deficit or 17.5% (severe water deficit soil water content. A sweet potato cultivar, ‘Japanese Yellow’, with water deficit tolerance attributes was the positive check in this study. Total soluble sugar content (sucrose, glucose, and fructose increased by 3.96-folds in ‘Tainung 57’ plants treated with 34 μM PBZ grown under 32.3% soil water content (SWC compared to the untreated plants, adjusting osmotic potential in the leaves and controlling stomatal closure (represented by stomatal conductance and transpiration rate. In addition, under the same treatment, free proline content (2.15 μmol g-1 FW increased by 3.84-folds when exposed to 17.5% SWC. PBZ had an improved effect on leaf size, vine length, photosynthetic pigment stability, chlorophyll fluorescence, and net photosynthetic rate; hence, delaying wilting symptoms and maintaining storage root yield (26.93 g plant-1 at the harvesting stage. A positive relationship between photon yield of PSII (ΦPSII and net photosynthetic rate was demonstrated (r2 = 0.73. The study concludes that soluble sugar and free proline enrichment in PBZ-pretreated plants may play a critical role as major osmoprotectant to control leaf osmotic potential and stomatal closure when plants were subjected to low soil water content, therefore, maintaining the physiological and morphological characters as well as storage root yield.

  3. The potential of computer vision, optical backscattering parameters and artificial neural network modelling in monitoring the shrinkage of sweet potato (Ipomoea batatas L.) during drying.

    Science.gov (United States)

    Onwude, Daniel I; Hashim, Norhashila; Abdan, Khalina; Janius, Rimfiel; Chen, Guangnan

    2018-03-01

    Drying is a method used to preserve agricultural crops. During the drying of products with high moisture content, structural changes in shape, volume, area, density and porosity occur. These changes could affect the final quality of dried product and also the effective design of drying equipment. Therefore, this study investigated a novel approach in monitoring and predicting the shrinkage of sweet potato during drying. Drying experiments were conducted at temperatures of 50-70 °C and samples thicknesses of 2-6 mm. The volume and surface area obtained from camera vision, and the perimeter and illuminated area from backscattered optical images were analysed and used to evaluate the shrinkage of sweet potato during drying. The relationship between dimensionless moisture content and shrinkage of sweet potato in terms of volume, surface area, perimeter and illuminated area was found to be linearly correlated. The results also demonstrated that the shrinkage of sweet potato based on computer vision and backscattered optical parameters is affected by the product thickness, drying temperature and drying time. A multilayer perceptron (MLP) artificial neural network with input layer containing three cells, two hidden layers (18 neurons), and five cells for output layer, was used to develop a model that can monitor, control and predict the shrinkage parameters and moisture content of sweet potato slices under different drying conditions. The developed ANN model satisfactorily predicted the shrinkage and dimensionless moisture content of sweet potato with correlation coefficient greater than 0.95. Combined computer vision, laser light backscattering imaging and artificial neural network can be used as a non-destructive, rapid and easily adaptable technique for in-line monitoring, predicting and controlling the shrinkage and moisture changes of food and agricultural crops during drying. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  4. Physical, chemical and sensory properties of brownies substituted with sweet potato flour (Ipomoea batatas L.) with addition of black cumin oil (Nigella sativa L.)

    Science.gov (United States)

    Ligarnasari, I. P.; Anam, C.; Sanjaya, A. P.

    2018-01-01

    Effect of addition black cumin oil on the physical (hardness) characteristics, chemical (water, ash, fat, protein, carbohydrate, antioxidant IC50, total phenol and active component) characteristics and sensory (flavor, taste, texture, overall) characteristics of brownies substituted sweet potato flour were investigated. Substituted brownies was added with 0.05%, 0.10%, 0.15%, 0.20% and 0.25% of nigella sativa oil. The result showed that water content, ash, protein, fat, total phenol were increased and carbohydrate, antioxidant IC50 was decreased by the addition of nigella sativa oil. Due to the sensory characteristics, panelist gave the high score for substituted brownies which was added 0.05% nigella sativa oil. The result showed that the best formula of substituted brownies which was added 0.05% of nigella sativa oil had 24.89% water content, 1.19% ash content, 7.54% protein content, 37.79% fat content, 53.06% carbohydrate contain, 1043.6 ppm IC50 antioxidant and 0.22% total phenol. The active component on the brownies using GCMS identification were palmitic acid, oleic acid, lauric acid, theobromine and vitamin E.

  5. Effect of including sweet potato (Ipomoea batatas Lam) meal in finishing pig diets on growth performance, carcass traits and pork quality.

    Science.gov (United States)

    Pietrosemoli, Silvana; Moron-Fuenmayor, Oneida Elizabeth; Paez, Angel; Villamide, Maria Jesús

    2016-10-01

    The partial replacement of a commercial concentrate at 10-20% and 15-30% (the first percentage of each dietary treatment corresponded to weeks 1-3 and the second to weeks 4-7 of the experiment, respectively) by sweet potato meal (SPM; 70% foliage: 30% roots) was evaluated for growth performance, carcass yield, instrumental and sensory pork quality using 36 commercial crossbred pigs (56.8 ± 1.3 kg initial body weight). Three dietary treatments were compared in a randomized complete block design. Most growth, carcass traits and pork quality variables were not affected by the SPM inclusion. Growth performance averaged 868 g/day and feed efficiency 0.24 kg/kg. However, feed intake increased 2.2% (P = 0.04) in pigs fed the 10-20% SPM diets, in a similar order of magnitude as the decrease in dietary energy. Despite an increase in gastrointestinal tract as a percent of hot carcass weight (+14.7%) (P = 0.03) with SPM inclusion, carcass yield averaged 69.4%. Conversely, decreases in loin yield (-4.2%) (P = 0.05), backfat thickness (-6.0%) (P < 0.01) and pork tenderness (-13%) (P = 0.02) were observed with 15-30% SPM inclusion. Results suggest that up to 20% SPM inclusion is a viable feed strategy for finishing pigs, easily replicable in small farm settings. © 2016 Japanese Society of Animal Science. © 2016 Japanese Society of Animal Science.

  6. Water-Deficit Tolerance in Sweet Potato [Ipomoea batatas (L.) Lam.] by Foliar Application of Paclobutrazol: Role of Soluble Sugar and Free Proline

    Science.gov (United States)

    Yooyongwech, Suravoot; Samphumphuang, Thapanee; Tisarum, Rujira; Theerawitaya, Cattarin; Cha-um, Suriyan

    2017-01-01

    The objective of this study was to elevate water deficit tolerance by improving soluble sugar and free proline accumulation, photosynthetic pigment stabilization, photosynthetic abilities, growth performance and storage root yield in sweet potato cv. ‘Tainung 57’ using a foliar application of paclobutrazol (PBZ). The experiment followed a Completely Randomized Block Design with four concentrations of PBZ: 0 (control), 17, 34, and 51 μM before exposure to 47.5% (well irrigation), 32.3% (mild water deficit) or 17.5% (severe water deficit) soil water content. A sweet potato cultivar, ‘Japanese Yellow’, with water deficit tolerance attributes was the positive check in this study. Total soluble sugar content (sucrose, glucose, and fructose) increased by 3.96-folds in ‘Tainung 57’ plants treated with 34 μM PBZ grown under 32.3% soil water content (SWC) compared to the untreated plants, adjusting osmotic potential in the leaves and controlling stomatal closure (represented by stomatal conductance and transpiration rate). In addition, under the same treatment, free proline content (2.15 μmol g-1 FW) increased by 3.84-folds when exposed to 17.5% SWC. PBZ had an improved effect on leaf size, vine length, photosynthetic pigment stability, chlorophyll fluorescence, and net photosynthetic rate; hence, delaying wilting symptoms and maintaining storage root yield (26.93 g plant-1) at the harvesting stage. A positive relationship between photon yield of PSII (ΦPSII) and net photosynthetic rate was demonstrated (r2 = 0.73). The study concludes that soluble sugar and free proline enrichment in PBZ-pretreated plants may play a critical role as major osmoprotectant to control leaf osmotic potential and stomatal closure when plants were subjected to low soil water content, therefore, maintaining the physiological and morphological characters as well as storage root yield. PMID:28848596

  7. PENGARUH KONSENTRASI GARAM TERHADAP WARNA, TOTAL ASAM DAN TOTAL BAKTERI ASAM LAKTAT PIKEL UBI JALAR UNGU (Ipomoea batatas var Ayamurasaki SELAMA FERMENTASI

    Directory of Open Access Journals (Sweden)

    Setiawan Setiawan

    2013-07-01

    Full Text Available The objective of this research was to investigate the influence of salt concentration during fermentation towards color stability of purple swee tpotato pickle. This experiment was arranged as a factorial design in completely randomized group design with two factors and three replications. The first factor was salt concentration (G consisted of four levels: 3%, 6%, 9%, and 12%. The second factor was the fermentation duration (H consisted of five levels; 0, 3, 6, 9, and 12 days. Data were analyzed using ANOVA further tested using polynomial orthogonal test at 1% and 5% level of significance. The results showed that the salt concentration and fermentation duration influenced total acid, pH and color stability. The best product was resulted from treatment of 3% salt concentration and 12 days fermentation duration with the characteristic as follows: total lactate acid bacteria (0.3 x 104 colony/mL, 1.6% total acid and the stable color score 4 (pink. Keywords: fermentation, pickle, purple sweet potato, salt

  8. VARIACIÓN DE LA DIFUSIVIDAD TÉRMICA DEL LOCHE (Cucurbita moschata Duch.), LA PAPA (Solanum tuberosum L.) Y EL CAMOTE (Ipomoea batatas L.) DURANTE EL CALENTAMIENTO

    OpenAIRE

    Solano-Corenjo, Miguel Ángel; Universidad Señor de Sipan; Vidaurre-Ruiz, Julio Mauricio; Universidad Señor de Sipan

    2017-01-01

    El objetivo de esta investigación fue evaluar la variación de la difusividad térmica del loche, la papa y el camote durante el calentamiento. También se evaluó la bondad de ajuste con los diferentes modelos predictivos que relacionan la variación de la difusividad térmica con respecto a la temperatura. Los valores de difusividad térmica efectiva se obtuvieron utilizando la curva de calentamiento de las muestras a las temperaturas de 40, 50, 60, 70, 80 y 90°C y se compararon los valores experi...

  9. CARACTERIZACIÓN ANATÓMICA DEL DESARROLLO DE LA RAÍZ RESERVANTE DE CAMOTES PERUANOS (IPOMOEA BATATAS (L. LAM., CONVOLVULACEAE

    Directory of Open Access Journals (Sweden)

    M. Marín

    2014-06-01

    Full Text Available La caracterización anatómica del desarrollo de la raíz reservante fue llevada a cabo en tres genotipos de camotes. En los estadios iniciales se encontraron diferencias en el desarrollo del xilema primario, incluyendo la formación de un parénquima medular. La actividad cambial secundaria contribuyó en gran medida al desarrollo del parénquima reservante, determinando un arreglo diferencial de los haces vasculares. Así mismo, fueron hallados granos simples y compuestos de almidón, siendo las dimensiones de los primeros asociadas a las mayores dimensiones celulares del parénquima. Uno de los genotipos evaluados presentó altos valores en el espesor del súber y granos de almidón (123,9 µm y 29,5 µm respectivamente. Se sugiere usar estas características como parámetros en la selección de genotipos de camote.

  10. Oxidative comparison of emulsion systems from fish oil-based structured lipid versus physically blended lipid with purple-fleshed sweet potato (Ipomoea batatas L.) extracts.

    Science.gov (United States)

    Gan, Lu-Jing; Yang, Dan; Shin, Jung-Ah; Kim, Sun-Ju; Hong, Soon-Taek; Lee, Jeung Hee; Sung, Chang-Keun; Lee, Ki-Teak

    2012-01-11

    The effects of the purple-fleshed sweet potato extract (PFSPE) on oxidation stabilities of a model oil-in-water emulsion prepared with enzymatically synthesized fish oil-soybean oil structured lipid (SL) versus physically blended lipid (PBL) without modification were evaluated. The anthocyanins in PFSPE were analyzed and identified by HPLC-MS. The fatty acid composition of SL was similar to that of PBL, except palmitic acid (1.48 in PBL and 9.61% in SL) and linoleic acid (62.47 in PBL and 49.58% in SL). Peonidin 3-caffeoylsophoroside-5-glucoside, peonidin-3-(6',6'-caffeoylferuloylsophoroside)-5-glucoside, peonidin-dicaffeoylsophoroside-5-glucoside, peonidin 3-(6',6"-caffeoyl-p-hydroxybenzoylsophoroside)-5-glucoside were identified as the major anthocyanin compounds in PFSPE. Different levels (200, 500, 1000 ppm) of PFSPE were added into both SL- and PBL-based emulsions, with 200 ppm catechin as comparison. Oxidation was monitored by measuring the peroxide value and thiobarbituric acid reactive substances. The antioxidant activity of PFSPE increased with an increased concentration, the concentration of 1000 ppm showed high antioxidant ability similar to that of catechin in both PBL- and SL-based oil-in-water emulsions. It is notable that the SL-based emulsion appeared to have better oxidative stability than the PBL-based emulsion.

  11. Water-Deficit Tolerance in Sweet Potato [Ipomoea batatas (L.) Lam.] by Foliar Application of Paclobutrazol: Role of Soluble Sugar and Free Proline.

    Science.gov (United States)

    Yooyongwech, Suravoot; Samphumphuang, Thapanee; Tisarum, Rujira; Theerawitaya, Cattarin; Cha-Um, Suriyan

    2017-01-01

    The objective of this study was to elevate water deficit tolerance by improving soluble sugar and free proline accumulation, photosynthetic pigment stabilization, photosynthetic abilities, growth performance and storage root yield in sweet potato cv. 'Tainung 57' using a foliar application of paclobutrazol (PBZ). The experiment followed a Completely Randomized Block Design with four concentrations of PBZ: 0 (control), 17, 34, and 51 μM before exposure to 47.5% (well irrigation), 32.3% (mild water deficit) or 17.5% (severe water deficit) soil water content. A sweet potato cultivar, 'Japanese Yellow', with water deficit tolerance attributes was the positive check in this study. Total soluble sugar content (sucrose, glucose, and fructose) increased by 3.96-folds in 'Tainung 57' plants treated with 34 μM PBZ grown under 32.3% soil water content (SWC) compared to the untreated plants, adjusting osmotic potential in the leaves and controlling stomatal closure (represented by stomatal conductance and transpiration rate). In addition, under the same treatment, free proline content (2.15 μmol g -1 FW) increased by 3.84-folds when exposed to 17.5% SWC. PBZ had an improved effect on leaf size, vine length, photosynthetic pigment stability, chlorophyll fluorescence, and net photosynthetic rate; hence, delaying wilting symptoms and maintaining storage root yield (26.93 g plant -1 ) at the harvesting stage. A positive relationship between photon yield of PSII (Φ PSII ) and net photosynthetic rate was demonstrated ( r 2 = 0.73). The study concludes that soluble sugar and free proline enrichment in PBZ-pretreated plants may play a critical role as major osmoprotectant to control leaf osmotic potential and stomatal closure when plants were subjected to low soil water content, therefore, maintaining the physiological and morphological characters as well as storage root yield.

  12. Chemical, sensory and rheological properties of porridges from processed sorghum (Sorghum bicolor), bambara groundnut (Vigna subterranea L. Verdc) and sweet potato (Ipomoea batatas) flours.

    Science.gov (United States)

    Nnam, N M

    2001-01-01

    The chemical, sensory and rheological properties of porridges made from blends of sprouted sorghum, bambara groundnuts and fermented sweet potatoes were examined. Sorghum and bambara groundnuts were sprouted for 48 h while sweet potatoes were fermented for the same period. Blends were formulated from the processed ingredients in the ratio of 60:40:0, 57:42:1, 55:44:1 and 52:46:2 (protein basis) of sorghum, bambara groundnuts and sweet potatoes. Porridges were prepared from the composite flours and the traditional sorghum complementary food. Standard assay methods were used to evaluate the flours for nutrient composition. The porridges were also tested for sensory properties and viscosity. Processing increased the levels of most of the nutrients evaluated. Relative to the sorghum traditional complementary food, the composite flours had higher levels of lipids, protein, ash, crude fiber and minerals (p < 0.05). The porridges from the composite flours were generally liked slightly by the panelists and were about seven times less viscous than the porridge from the traditional sorghum complementary food. Use of the composite flours, particularly the 52:46:2 blend, as a traditional complementary food should be encouraged in Nigeria especially with the increasing cost of commercial complementary foods.

  13. Light interception, utilisation and conversion efficiencies and yield performance of sweet potato, Ipomoea batatas, grown from IBA-treated basal and untreated terminal vine cuttings

    International Nuclear Information System (INIS)

    Nzima, M.D.S.

    1985-01-01

    Sweet potatoes grown from IBA-treated basal vines produced a significantly higher total yield of 28.99 t ha -1 and marketable tubers 23.54 t ha -1 , than those grown from untreated terminal vine cuttings which produced 22.43 and 17.46 t ha -1 of total and marketable tubers respectively. Field measurements indicated that IBA-treated basal vine plants were significantly less efficient in light interception, yet this treatment produced a higher proportion (31.20%) of large tubers, compared with untreated terminal vine plants (14.64%). Untreated terminal vine plants intercepted 95% of the incident solar radiation three weeks earlier than IBA-treated basal vine plants. However, the treatment increased the efficiency of converting light energy to chemical energy (Ec%) and of utilising this chemical energy to produce plant dry matter (Eu%) through an increased 'sink' demand. The practical implications of the results and future research are outlined

  14. Physical Characteristics of White Sweet Potato (Ipomoea batatas L.), Rice (Oryza sativa L.), and Tapioca (Manihot esculenta) Flours - Based Seasoning Composite Flour

    Science.gov (United States)

    Alfani, NNA; Ishartani, D.; Anam, C.; Praseptiangga, D.; Manuhara, G. J.

    2017-04-01

    The objective of this study was to determine the physical characteristics of seasoning composite flour that made from white sweet potato, rice, and tapioca flours, and determined the best formula of seasoning composite flour. A completely randomized design (CRD) with formula as the single factor was used. The data were analyzed by one-way ANOVA method and followed by Duncan Multiple Range Test (DMRT) at significance 5% if there was a significant difference. The best formula of seasoning composite flour was 30% tapioca flour, 30% rice flour, and 40% white sweet potato flour. The physical characteristics of the best formula were 5.689 ml/g of swelling power, 2.681 g/g of water absorption capacity, 0.887 ml/g of oil absorption capacity, and 22.03% cooking loss. Physical characteristics of the best seasoning composite flour were significantly different from the commercial seasoning flour and showed a better cooking loss, oil absorption capacity, and swelling power than commercial seasoning flour.

  15. Use of abscisic acid, mannitol and salt concentration decrease in the culture medium in the in vitro conservation of Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Angel Espinosa Reyes

    2002-01-01

    Full Text Available Nodal segment from in vitro plants of Jewel clone sweet potato were culture in the following culture medias: a Murashige and Skoog (1962 salts supplemented with abscisic acid (0.0, 1.0, 5.0 y 10.0 mg.l-1, b Murashige and Skoog (1962 salts supplemented with mannitol (0.0, 10.0 15.0 y 20.0 g.l-1 and c different Murashige and Skoog (1962 salts concentration (100, 75, 50 and 25% were used. Survival during conservation was high in all experiment. The 5.0 and 10.0 mg.l-1 abscisic acid strongly inhibited the plants growth. It was reached a decrease of growth when using mannitol in the culture medium. The media with 25% and 50% MS salts concentration caused a low of plants growth. When cultured in fresh culture medium it was achieved a high recovery ratio of conserved material. Key words: sweet potato, minimal growth, phyto resources, biodiversity

  16. CHEMICAL COMPOSITION AND IN VITRO DRY MATTER DIGESTIBILITY OF VINES AND ROOTS OF FOUR SWEET POTATO (ipomoea batatas VARIETIES GROWN IN SOUTHERN ETHIOPIA

    Directory of Open Access Journals (Sweden)

    Gebreegziabher Zereu Hadgu

    2014-12-01

    Full Text Available Chemical composition and in vitro true dry matter digestibility (IVTDMD of vine and its fractions (leaf, petiole and stem and roots of two early maturing (Belela and Temesgen and two medium maturing varieties (Beletech and Tulla were investigated. Vine and root samples from each variety were harvested from triplicate plots (5.1m x 4.8m. Vine samples were partitioned in two equal parts: one part analyzed as whole vine and the second part was fractionated into leaf, petiole and stem. Differences in chemical composition and IVTDMD between vine and its fractions (leaf, petiole and stem were significant (p<0.05. Leaf had crude protein (CP content of 26.1, 22.3, 20.5 and 28.4%DM; Neutral detergent fiber (NDF 25.4, 25.3, 30.7 and 30.2%DM and IVTDMD 90.7, 90.6, 87.5 and 90.1%; petiole had CP of 7.9, 12.1, 7.7and 10.0%DM; NDF 27.8, 29.4, 28.5 and 27.9%DM and IVTDMD 93.4, 93.4, 91.4 and 91.5% and stem had CP of 8.2, 10.9, 8.3 and 10.3%DM; NDF 38.5, 37.5, 40.4%DM and 40.2 and IVTDMD 78.2, 77.8, 74.4 and 76.3%, respectively, for Belela, Temesgen, Beletech and Tulla. In vine CP of 18.7, 18.1, 15.5 and 17.3%DM; NDF 32.7, 31.4, 38.3%DM and 36.9 and IVTDMD 86.2, 86.3, 83.1 and 83.5%, respectively, for Belela, Temesgen, Beletech and Tulla were found. There was also significant (p<0.05 variations in CP and NDF but not (p<0.05 in IVTDMD of roots between varieties. Root CP of 6.9, 7.7, 6.8 and 6.3 % DM; NDF 7.2, 6.0, 6.5 and 6.3 %DM and IVTDMD 94.3, 94.5, 94.9 and 94.6%, respectively, for Belela, Temesgen, Beletech and Tulla were found. In conclusion, Belela and Temesgen had better quality than Beletech and Tulla in most measured parameters.

  17. Accumulation of lead and arsenic in Malabar spinach (Basella alba L.) and sweet potato (Ipomoea batatas L.) leaves grown on urban and orchard soils.

    Science.gov (United States)

    Migration of people of different ethnic backgrounds to U.S. urban areas has resulted in different ethnic vegetable crops being grown in urban gardens. There are concerns that some ethnic vegetable crops may accumulate heavy metals when grown on urban soils. The objective of this study was to evalua...

  18. NAS CULTIVARES DE BATATA ASTERIX E ATLANTIC POR MARCADORES MORFOLÓGICOS E MICROSSATÉLITES

    OpenAIRE

    Gisele Santiago

    2011-01-01

    A batata (Solanum tuberosum L.) ocupa o quarto lugar em nível mundial em produção, sendo superada apenas pelo trigo, arroz e milho. A cultura da batata é propagada de forma vegetativa e, devido à suscetibilidade a doenças, é imprescindível o emprego da cultura de ápices caulinares para efetuar limpeza clonal e, na sequência, da micropropagação das partes aéreas para a obtenção de mudas livres de patógenos para satisfazer a demanda dos agricultores. Entretanto, a cultura de tecidos pode induzi...

  19. A CULTURA DA BATATA-DOCE: CULTIVO, PARENTESCO E RITUAL ENTRE OS KRAHÔ

    OpenAIRE

    Lima, Ana Gabriela Morim de

    2017-01-01

    Resumo O artigo tematiza as relações de parentesco cotidianas e rituais entre humanos e plantas por meio de uma etnografia do ciclo de vida da batata-doce, cultivada pelos Krahô: desde o plantio, passando pelo crescimento, até a colheita, quando ocorre a “Festa da Batata”. O “aparentamento” mútuo entre as batatas e seus donos humanos envolve engajamentos corporais, afetivos e estéticos, implicando relações sociais de criação, troca, predação, transmissão de conhecimento xamânico e ritual. As ...

  20. Growth of sweetpotato cultured in the newly designed hydroponic system for space farming

    Science.gov (United States)

    Kitaya, Y.; Hirai, H.; Wei, X.; Islam, A. F. M. S.; Yamamoto, M.

    Life support of crews in long-duration space missions for other planets will be highly dependent on amounts of food, atmospheric O2 and clean water produced by plants. Therefore, the space farming system with scheduling of crop production, obtaining high yields with a rapid turnover rate, converting atmospheric CO2 to O2 and purifying water should be established with employing suitable plant species and cultivars and precisely controlling environmental variables around plants grown at a high density in a limited space. In this study, we developed a new hydroponic method for producing tuberous roots and fresh edible leaves and stems of sweetpotato. In the first experiment, we examined the effects of water contents in the rooting substrate on growth and tuberous root development of sweetpotato. The rooting substrates made with rockwool slabs were inclined in a culture container and absorbed nutrient solution from the lower end of the slabs by capillary action. Tuberous roots developed on the lower surface of the rockwool slabs. The tuberous roots showed better growth and development at locations farther from the water surface on the rockwool slabs, which had lower water content. In the second experiment, three sweetpotato cultivars were cultured in a hydroponic system for five months from June to November under the sun light in Osaka, Japan as a fundamental study for establishing the space farming system. The cultivars employed were ‘Elegant summer’, ‘Kokei-14’ and ‘Beniazuma’. The hydroponic system mainly consisted of culture containers and rockwool slabs. Dry weights of tuberous roots developed in the aerial space between the rockwool slab and the nutrient solution filled at the bottom of the culture container were 0.34, 0.45 and 0.23 kg/plant and dry weights of the top portion (leaves, petioles and stems) were 0.42, 0.29 and 0.61 kg/plant for ‘Elegant summer’, ‘Kokei-14’ and ‘Beniazuma’, respectively. Young stems and leaves as well as

  1. Alkaloidal components in the poisonous plant, Ipomoea carnea (Convolvulaceae).

    Science.gov (United States)

    Haraguchi, Mitsue; Gorniak, Silvana L; Ikeda, Kyoko; Minami, Yasuhiro; Kato, Atsushi; Watson, Alison A; Nash, Robert J; Molyneux, Russell J; Asano, Naoki

    2003-08-13

    Natural intoxication of livestock by the ingestion of Ipomoea carnea (Convolvulaceae) sometimes occurs in tropical regions of the world. Polyhydroxylated alkaloids were isolated from the leaves, flowers, and seeds of the poisonous plant and characterized. Chromatographic separation of the leaf extract resulted in the isolation of swainsonine (1), 2-epi-lentiginosine (2), calystegines B(1) (3), B(2) (4), B(3) (5), and C(1) (6), and N-methyl-trans-4-hydroxy-l-proline (7). The contents of 1 in the fresh leaves and flowers were 0.0029 and 0.0028%, respectively, whereas the contents of 1, 3, and 4 in the seeds were approximately 10 times higher than those in the leaves and flowers. Alkaloids 3, 4, and 6 showed a potent inhibitory activity toward rat lysosomal beta-glucosidase, with IC(50) values of 2.1, 0.75, and 0.84 microM, respectively, and alkaloid 5 was a moderate inhibitor of alpha- and beta-mannosidases. Although alkaloid 1 is known as a powerful inhibitor of lysosomal alpha-mannosidase (IC(50) = 0.02 microM), alkaloid 2, which has been thought to be an intermediate in the biosynthesis of 1, was also a potent inhibitor of alpha-mannosidase with an IC(50) value of 4.6 microM.

  2. The role of stamens in ethylene production in Ipomoea nil

    International Nuclear Information System (INIS)

    Kiss, H.G.

    1989-01-01

    Ethylene production inhibits filament and corolla growth during young stages in flower development, and it promotes corolla unfolding and senescence in Ipomoea nil. Initial studies with the in vitro application of gibberellic acid (GA 3 ), demonstrated that decreased filament growth occurred when the anthers remained attached to the filaments during the young stages in development. The removal of the anthers from intact plants did not enhance filament growth until the synthesis of wound ethylene was inhibited by applied aminoethoxyvinylglycine (AVG) or cobalt chloride. It was hypothesized that the anthers were source tissues and that the filaments were transport vectors for the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and indole-3-acetic acid (IAA) to regulate growth events in the various floral organs. To test this hypothesis, endogenous IAA and ACC and ethylene production were measured by enzyme linked immunosorbent assay (ELISA) or gas chromatography. The transport of 14 C-IAA and 14 C-ACC through filament segments and filaments within intact flower buds also was examined during flower development

  3. Secretory structures of Ipomoea asarifolia: anatomy and histochemistry

    Directory of Open Access Journals (Sweden)

    Fabiano M. Martins

    2012-02-01

    Full Text Available Ipomoea asarifolia (Desr. Roem. & Schult., Convolvulaceae, is a weed that infests agricultural areas and is toxic to cattle. In spite of its toxicity, the leaves of this plant are used in traditional remedies in the state of Bahia, Brazil. The present work describes the leaf anatomy of I. asarifolia and characterizes the exudates of its secretory structures. The leaves have a unistratified epidermis composed of ordinary cells with straight to slightly sinuous anticlinal walls and thin cuticles. Paracytic stomata are found on both surfaces of the leaves at the same level as the ordinary epidermal cells. Trichomes producing polysaccharide secretions occur on the petiole and leaf blade and are considered colleters. The mesophyll is dorsiventral and the vascular bundle of the central vein is bicollateral. Two opposed nectaries occur on the petiole near the leaf blade. Each nectary is composed of a small canal with internal ramifications and numerous secretory trichomes. The laticiferous glands are articulated, not anastomosed, and are composed of large diameter cells with thin cell walls. The secretions of the laticiferous glands are lipidic.

  4. Physical properties and sixth graders' acceptance of an extruded ready-to-eat sweetpotato breakfast cereal

    Science.gov (United States)

    Dansby, M. Y.; Bovell-Benjamin, A. C.

    2003-01-01

    Extruded ready-to-eat breakfast cereals (RTEBCs) were made from varying levels of sweetpotato flour (SPF), whole-wheat bran (WWB), and extrusion cooking. Moisture, protein, and ash contents were lower in the 100% SPF than the 100% WWB. Carbohydrate, beta-carotene, and ascorbic acid contents were higher in the 100% SPF. Fat, thiamin, riboflavin contents, bulk densities, and the water absorption index were similar for the cereals. However, the expansion ratio was highest in the 100% SPF cereal. The 100% WWB had the lightest color and most fibrous morphology. Extruded RTEBC containing 100% SPF and 75%/25% SPF/WWB were well-liked and acceptable to sixth graders attending an elementary school in Auburn, Alabama, but the 100% WWB was unacceptable.

  5. Sweet potato (Ipomea batatas) tuber - potential oral anti-diabetic agent

    African Journals Online (AJOL)

    Diabetes is one of the ailments that traditional healers had claimed cure capabilities by the use of some medicinal plants. Sweet potato (Ipomea batatas) is one of such plants in which much claims had been made. However, this claim has not previously been scientifically validated and experimentally assessed.

  6. Agricultural marketing in a country in transition : case of sweetpotato products in Sichuan, P.R. China

    OpenAIRE

    Zhang, X.

    1999-01-01

    China's achievements in its reform from a centrally planned economy toward a marketing economy during the last two decades are substantial. Although it still has a long way to go to reach its targeted aims, half of China's economy is now 'marketised, or is responsive to market forces (Lawrence, 1998). The influence of the transition on agricultural marketing is significant, particularly when the reforms were initiated by farmers and started in rural areas. The sweetpotato sector, one...

  7. RESISTENCIA MECÁNICA DEL SUELO A LA PENETRACIÓN DE RAÍCES Y FORMA DE LA RAÍZ RESERVANTE DE LA BATATA A PARTIR DEL DESCRIPTOR DE HUAMÁN

    Directory of Open Access Journals (Sweden)

    Maiby Pérez

    2016-01-01

    Full Text Available Se estableció un ensayo para determinar la influencia de la resistencia mecánica a la penetración de raíces (RP, en un suelo afectado por la labranza y por la distancia de siembra, sobre la forma de la raíz reservante del cultivo batata ( Ipomoea batatas (L. Lamb. El diseño del experimento se basó en un arreglo en franjas, donde los sistemas de labranza (SL fueron asig - nados a las parcelas grandes y las densidades de siembra (DS a las franjas. Los SL consistieron en: 1 labranza vertical profunda con cincel y 3 pases de rastra (LC; 2 profunda con arado de disco y 3 pases de rastra (LA; 3 media con 3 pases de rastra de disco (LCo; y 4 mínima con un pase de rastra de disco (LM. Con 4 profun - didades de trabajo de 18, 23, 12 y 7 cm, respecti - vamente. Las DS fueron de 0,25 cm (D1 y 0,15 cm (D2 en la hilera. La RP fue superior en los estratos del suelo a profundidades de 5 a 15 y 15 a 25 cm en los tratamientos de LM, (44 y 37%, respectivamente, respecto al promedio obser - vado en el resto de los tratamientos en los estra - tos mencionados. Hubo escasa diferenciación entre los sistemas de labranza media y profunda (LCo, LC, LA para RP. Se encontraron formas redondeadas preferiblemente en los tratamientos de LA; elíptica y redonda, elíptica bajo LC; y redonda y redonda elíptica en LCo y LM. No se observó una mayor restricción del crecimiento longitudinal de la raíz del cultivo en el suelo con mayor dureza bajo los tratamientos de LM. Esto en parte explicado a partir de los valores no limitantes a la penetración de raíces (< 2 MPa del suelo en los diferentes tratamientos.

  8. Sweetpotato-based complementary food would be less inhibitory on mineral absorption than a maize-based infant food assessed by compositional analysis.

    Science.gov (United States)

    Amagloh, Francis Kweku; Brough, Louise; Weber, Janet L; Mutukumira, Anthony N; Hardacre, Allan; Coad, Jane

    2012-12-01

    The availability of micronutrients from sweetpotato-based complementary foods (CFs): oven-toasted and roller-dried ComFa, and from a maize-based infant food, enriched Weanimix, was compared using phytate/mineral molar ratios, polyphenols and β-carotene levels. The phytate/calcium, iron and zinc molar ratios of approximately 0.17, 1 and 15 predict better absorption of calcium, iron and zinc respectively. Generally, the sweetpotato-based CFs had at least half the phytate/mineral ratios of enriched Weanimix. The phytate/iron ratio in both the sweetpotato- and the maize-based CFs was greater than 1. Only the ComFa formulations had phytate/zinc ratio lower than 15. The level of polyphenol (iron inhibitor) was similar for the formulations. Only the sweetpotato-based CFs contained measurable levels of β-carotene, a possible iron enhancer. The lower phytate/mineral ratios and the β-carotene level of the sweetpotato-based CFs suggest that calcium, iron and zinc absorption could be better from them than from the maize-based infant food.

  9. A New Resin Glycoside, Muricatin IX, from the Seeds of Ipomoea muricata.

    Science.gov (United States)

    Ono, Masateru; Taketomi, Saki; Kakiki, Yuichi; Yasuda, Shin; Okawa, Masafumi; Kinjo, Junei; Yoshimitsu, Hitoshi; Nohara, Toshihiro

    2016-01-01

    A new resin glycoside, named muricatin IX (1), was isolated from the seeds of Ipomoea muricata (L.) JACQ. (Convolvulaceae). The structure of 1 was determined on the basis of spectroscopic data as well as chemical evidence. Compound 1 is the first representative of resin glycosides in which an organic acid connects the sugar moiety and the aglycone moiety to form macrocyclic ester ring.

  10. Induction of callogenesis in Ipomoea obscura (L.) Ker- Gawl, a little ...

    African Journals Online (AJOL)

    ajl yemi

    seeds of Ipomoea obscura 62: 1257-1263. Kirtikar KR, Basu BD (1999). Indian Medicinal plants. Text Vol. III. Narayanaswamy S (2005). Plant cell and tissue Culture, Tata Mc-Graw. Hill publishing Co. Ltd. 6th reprint Plant Cell Tissue Cult. pp. 27-28. Pankaj, Oudhia Research Note @ (2001), (2002), (2003), Botanical. com.

  11. Wound healing and dry matter content of orange-fleshed sweetpotato cultivars as influenced by curing methods

    Directory of Open Access Journals (Sweden)

    Atuna Richard A.

    2017-02-01

    Full Text Available Curing in sweetpotato is a crucial pre- or postharvest practice that could guarantee improved shelf life,but rarely practised by sweetpotato farmers in Sub-Saharan Africa, principally due to lack of knowledge. Wound healing ability of cultivars has been associated with good root storability. In this study, two orange fleshed sweetpotato cultivars (Apomuden and Nane were either cured in-ground by dehaulming prior to harvest or field-piled over a seven-day period to study their responses to wound healing and changes in dry matter content. Apomuden is a low dry matter content(19% variety in Ghana while Nane is a high dry matter content (27% farmer cultivar under evaluation for formal release. A potato peeler was used to deliberately create the wounds on 21 storage roots. The curing treatment was applied and the subsequent post-treatment quality status of the storage roots was monitored daily over a seven-day period. Wound healing ability was scored as follows: 0 = no lignification, 0.5 = patchy lignification and1= complete lignification. Wound healing ability score was not significantly different for Apomuden and Nane (0.83,0.78, respectively; p = 0.120. However, storage roots curedby field-piled curing method resulted in significantly better wound healing ability than dehaulming (0.86, 0.75,respectively, p = 0.001. Over the seven-day curing period,Nane had a significantly higher and stable dry mattercontent compared with Apomuden (p = 0.008, whose dry matter content was lower and fluctuating. The field-piled curing resulted in higher (p = 0.020 dry matter content,24%, compared with in-ground curing (22%. The field piled curing method, which can easily be adopted by sweetpotato farmers, increased the dry matter content of the storage roots; therefore, it could potentially reduce the post-harvest losses in sweetpotato. The high dry matter content of Nane is a desirable root quality attribute for orange-fleshed cultivars and could augment existing

  12. Rhizobium ipomoeae sp. nov., isolated from a water convolvulus field.

    Science.gov (United States)

    Sheu, Shih-Yi; Chen, Zih-Han; Young, Chiu-Chung; Chen, Wen-Ming

    2016-04-01

    A bacterial strain, designated shin9-1T, was isolated from a water sample taken from a water convolvulus field in Taiwan and characterized using a polyphasic taxonomical approach. Cells of strain shin9-1T were aerobic, Gram-stain-negative, rod-shaped and surrounded by a thick capsule and formed cream-coloured colonies. Growth occurred at 10-45 °C (optimum, 30 °C), with 0-3.0% NaCl (optimum, 0.5%) and at pH 7.0-9.0 (optimum, pH 7.0). Strain shin9-1T did not form nodules on a legume plant, Macroptilium atropurpureum, and the nodulation genes nodA, nodC and the nitrogenase reductase gene nifH were not detected by PCR. Phylogenetic analyses based on 16S rRNA and three housekeeping gene sequences (recA, atpD and rpoB) showed that strain shin9-1T belonged to the genus Rhizobium. Strain shin9-1T had the highest level of 16S rRNA gene sequence similarity with respect to Rhizobium daejeonense L61T (97.6 %). The major fatty acid of strain shin9-1T was C18:1ω7c. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine and several uncharacterized lipids. The DNA G+C content was 58.3 mol%. The DNA-DNA relatedness of strain shin9-1T with respect to recognized species of the genus Rhizobium was less than 70%. Phenotypic characteristics of the novel strain also differed from those of the most closely related species of the genus Rhizobium. On the basis of the phylogenetic inference and phenotypic data, strain shin9-1T should be classified as a representative of a novel species, for which the name Rhizobium ipomoeae sp. nov. is proposed. The type strain is shin9-1T (=LMG 27163T=KCTC 32148T).

  13. Study of antidiabetic activity of white skinned sweet potato (Ipomoea batatas L.). Comparison of normal and streptozotocin induced diabetic rats and hereditary diabetic mice; Hakukansho (Ipomoea batatas L.) no kotonyobyo sayo. Seijo rat, streptozotocin tonyobyo rat, oyobi shizen hassho tonyobyo mausu ni taisuru sayo hikaku

    Energy Technology Data Exchange (ETDEWEB)

    Kusano, S.; Abe, H.; Okada, A. [Fuji Sangyo Co. Ltd., Kagawa (Japan)

    1998-09-01

    As a result of study on the effect of white skinned sweet potato (WSSP), it is found that the potato has potential hypoglycemic activity in streptozotocin (STZ) induced diabetic rats and hereditary diabetic mice (db/db). This report is the first one showing the antidiabetic effect of WSSP. Antidiabetic activity of WSSP in STZ induced diabetic rats and hereditary diabetic mice is shown. WSSP increases serum insulin levels of STZ induced diabetic rats like the tolbutamide treatment, and controls blood glucose after oral glucose load. WSSP decreases high blood glucose value with the decrease of insulin value in hereditary diabetic mice, and improves glucose tolerance. Those mice are models showing insulin resistance, and it is assumed from the effect on those diabetic models that blood glucose value is decreased by the improvement of insulin resistance. 37 refs., 4 figs., 2 tabs.

  14. Floral biology and breeding system of three Ipomoea weeds Biologia floral e sistema reprodutivo de três espécies daninhas de Ipomoea

    Directory of Open Access Journals (Sweden)

    R.C.S. Maimoni-Rodella

    2007-03-01

    Full Text Available The floral biology of three weeds, Ipomoea cairica, I. grandifolia and I. nil (Convolvulaceae, was studied in Botucatu and Jaboticabal, São Paulo, in southeastern Brazil. The three species are melittophilous, with a varied set of floral visitors, but with some overlapping. Cluster analysis using Jacquard similarity index indicated a greater similarity among different plant species in the same locality than among the populations at different places, in relation to floral visitor sets. The promiscuous and opportunistic features of the flowers were shown, with such type of adaptation to pollination being advantageous to weeds since pollinator availability is unpredictable at ruderal environments.A biologia floral de Ipomoea cairica, I. grandifolia e I. nil - plantas daninhas da família Convolvulaceae - foi estudada em Botucatu e Jaboticabal, Estado de São Paulo, Brasil. As três espécies são melitófilas, apresentando conjuntos de visitantes florais bastante diversificados, embora haja alguma sobreposição entre eles. Com relação aos visitantes florais, a análise de agrupamento, empregando-se o índice de similaridade de Jaccard, indicou maior similaridade entre diferentes espécies de Ipomoea ocorrentes no mesmo local do que entre populações da mesma espécie em diferentes localidades. O caráter promíscuo e oportunista da adaptação à polinização, presente nessas espécies, foi demonstrado, sendo essa adaptação vantajosa para plantas daninhas, uma vez que em ambientes ruderais a disponibilidade de polinizadores é imprevisível.

  15. Yield and gas exchange ability of sweetpotato plants cultured in a hydroponic system

    Science.gov (United States)

    Kitaya, Y.; Hirai, H.; Saiful Islam, A. F. M.; Yamamoto, M.

    Life support of crews in space is greatly dependent on the amounts of food atmospheric O 2 and clean water produced by plants Therefore the space farming systems with scheduling of crop production obtaining high yields with a rapid turnover rate converting atmospheric CO 2 to O 2 and purifying water should be established with employing suitable plant species and varieties and precisely controlling environmental variables around plants grown at a high density in a limited space In this study three sweetpotato varieties were cultured in a newly developed hydroponic system and the yield the photosynthetic rate and the transpiration rate were compared on the earth as a fundamental study for establishing the space farming systems The varieties were Elegant summer Koukei 14 and Beniazuma The hydroponic system mainly consisted of water channels and rockwool boards A growing space for roots was made between the rockwool board and nutrient solution in the water channel Storage roots were developed on the lower surface of the rockwool plates Fresh weights of the storage roots were 1 6 1 2 and 0 6 kg plant for Koukei 14 Elegant summer and Beniazuma respectively grown for five months from June to October under the sun light in Osaka Japan Koukei 14 and Elegant summer produced greater total phytomass than Beniazuma There were positive correlations among the total phytomass the net photosynthetic rate and the transpiration rate Young stems and leaves as well as storage roots of Elegant summer are edible Therefore Elegant-summer

  16. Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance.

    Directory of Open Access Journals (Sweden)

    Zhi Wang

    Full Text Available Alfalfa (Medicago sativa L., a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. In this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the IbOr gene into alfalfa (cv. Xinjiang Daye under the control of an oxidative stress-inducible peroxidase (SWPA2 promoter through Agrobacterium tumefaciens-mediated transformation. Among the 11 transgenic alfalfa lines (referred to as SOR plants, three lines (SOR2, SOR3, and SOR8 selected based on their IbOr transcript levels were examined for their tolerance to methyl viologen (MV-induced oxidative stress in a leaf disc assay. The SOR plants exhibited less damage in response to MV-mediated oxidative stress and salt stress than non-transgenic plants. The SOR plants also exhibited enhanced tolerance to drought stress, along with higher total carotenoid levels. The results suggest that SOR alfalfa plants would be useful as forage crops with improved nutritional value and increased tolerance to multiple abiotic stresses, which would enhance the development of sustainable agriculture on marginal lands.

  17. Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q.

    Science.gov (United States)

    Xie, Wen; Chen, Chunhai; Yang, Zezhong; Guo, Litao; Yang, Xin; Wang, Dan; Chen, Ming; Huang, Jinqun; Wen, Yanan; Zeng, Yang; Liu, Yating; Xia, Jixing; Tian, Lixia; Cui, Hongying; Wu, Qingjun; Wang, Shaoli; Xu, Baoyun; Li, Xianchun; Tan, Xinqiu; Ghanim, Murad; Qiu, Baoli; Pan, Huipeng; Chu, Dong; Delatte, Helene; Maruthi, M N; Ge, Feng; Zhou, Xueping; Wang, Xiaowei; Wan, Fanghao; Du, Yuzhou; Luo, Chen; Yan, Fengming; Preisser, Evan L; Jiao, Xiaoguo; Coates, Brad S; Zhao, Jinyang; Gao, Qiang; Xia, Jinquan; Yin, Ye; Liu, Yong; Brown, Judith K; Zhou, Xuguo Joe; Zhang, Youjun

    2017-05-01

    The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future 'pan-genomic' comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management. © The Author 2017. Published by Oxford University Press.

  18. Resistance Monitoring for Eight Insecticides on the Sweetpotato Whitefly (Hemiptera: Aleyrodidae) in China.

    Science.gov (United States)

    Wang, Shaoli; Zhang, Youjun; Yang, Xin; Xie, Wen; Wu, Qingjun

    2017-04-01

    The sweetpotato whitefly, Bemisia tabaci (Gennadius), is an important pest of many crops worldwide. Because control of B. tabaci still depends on the application of insecticides in China, monitoring the insecticide resistance of B. tabaci populations is essential for achieving control and for managing resistance. In this study, field populations of B. tabaci on vegetables were collected in three regions of China in 2011, 2012, and 2013. The resistance of these populations (all of which were determined to belong to biotype Q) to eight insecticides (abamectin, spinetoram, imidacloprid, thiamethoxam, acetamiprid, nitenpyram, chlorpyrifos, and bifenthrin) was assessed by the leaf-dip method. No resistance to abamectin and spinetoram was detected. All of the B. tabaci populations exhibited resistance to neonicotinoid insecticides; the resistance was 3.6- to 125.0-fold greater than that of a susceptible reference strain. The traditional insecticides chlorpyrifos and bifenthrin had very low toxicity. Bemisia tabaci specimens in some regions exhibited annual differences in resistance to some of the insecticides. The data presented will be helpful for making decisions on the proper insecticide usage in the field. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  19. Specialised Sweetpotato Vine Multiplication in Lake Zone, Tanzania: What “Sticks” and What Changes?

    Directory of Open Access Journals (Sweden)

    McEwan Margaret A.

    2017-02-01

    Full Text Available In Lake Zone, Tanzania, farmers were trained to multiply and distribute quality sweetpotato planting material. The objectives of this study were to assess changes in skills and practices among the trained farmers as vine multiplication became a specialized task. Nine months after the project ended, all 88 decentralized vine multipliers (DVMs operating as groups (72% or individuals (28% were visited and qualitative data on their current multiplication practices collected through a questionnaire, checklists and observations. Results showed that 69% of DVMs were still multiplying vines, but less than half were using the seed production technologies promoted by the project. 34% used rapid multiplication beds; 61% used conventional plant spacing on ridges for roots and vines and 5% used both. As the vine multiplication cycle became a specialised activity, the multiplication and root production cycles were separated. Vines were treated differently in terms of site selection, length of cutting and spacing, depending on whether the objective of their use was for high root or high vine production. Capacity building of specialised vine multipliers and scaling-up seed interventions should consider the implications of skilling and task segregation in a broader context based on society’s choice of technologies and agrarian change.

  20. Toxicity of insecticides to the sweetpotato whitefly (Hemiptera: Aleyrodidae) and its natural enemies.

    Science.gov (United States)

    Bacci, Leandro; Crespo, André L B; Galvan, Tederson L; Pereira, Eliseu J G; Picanço, Marcelo C; Silva, Gerson A; Chediak, Mateus

    2007-07-01

    Efficient chemical control is achieved when insecticides are active against insect pests and safe to natural enemies. In this study, the toxicity of 17 insecticides to the sweetpotato whitefly, Bemisia tabaci (Gennadius), and the selectivity of seven insecticides to natural enemies of this insect pest were evaluated. To determine the insecticide toxicity, B. tabaci adults were exposed to abamectin, acephate, acetamiprid, cartap, imidacloprid, malathion, methamidophos, bifenthrin, cypermethrin, deltamethrin, esfenvalerate, fenitrothion, fenpropathrin, fenthion, phenthoate, permethrin and trichlorphon at 50 and 100% of the field rate (FR), and to water (untreated control). To determine the insecticide selectivity, adults of Encarsia sp., Acanthinus sp., Discodon sp. and Lasiochilus sp. were exposed to abamectin, acephate, acetamiprid, cartap, imidacloprid, malathion and methamidophos at 50 and 100% FR, and to water. Groups of each insect species were exposed to kale leaves preimmersed in each treatment under laboratory conditions. Mortality of exposed individuals was recorded 24 h after treatment. Cartap and imidacloprid at 50 and 100% FR and abamectin and acetamiprid at 100% FR showed insecticidal activity to B. tabaci adults. Abamectin at 50 and 100% FR was the least insecticidal compound to the natural enemies Acanthinus sp., Discodon sp. and Lasiochilus sp. The present results suggest that abamectin at 100% FR may decrease B. tabaci field populations but can still be harmless to predators. Implications of these results within an integrated pest management context are discussed. Copyright (c) 2007 Society of Chemical Industry.