Relationship of intertidal surface sediment chlorophyll concentration to hyper-spectral reflectance and chlorophyll fluorescence

NARCIS (Netherlands)

Kromkamp, J.C.; Morris, E.P.; Forster, R.M.; Honeywill, C.; Hagerthey, S.; Paterson, D.M.

2006-01-01

Estimating biomass of microphytobenthos (MPB) on intertidal mud flats is extremely difficult due to their patchy occurrence, especially at the scale of an entire mud flat. We tested two optical approaches that can be applied in situ: spectral reflectance and chlorophyll fluorescence. These two

Phytoplankton chlorophyll

NARCIS (Netherlands)

van de Poll, W.H.; Kulk, G.; Timmermans, K.R.; Brussaard, C.P.D.; van der Woerd, H.J.; Kehoe, M.J.; Mojica, K.D.A.; Visser, R.J.W.; Rozema, P.D.; Buma, A.G.J.

2013-01-01

Relationships between sea surface temperature (SST, > 10 m) and vertical density stratification, nutrient concentrations, and phytoplankton biomass, composition, and chlorophyll a (Chl a) specific absorption were assessed in spring and summer from latitudes 29 to 63 degrees N in the northeast

Revisiting chlorophyll extraction methods in biological soil crusts - methodology for determination of chlorophyll a and chlorophyll a + b as compared to previous methods

Science.gov (United States)

Caesar, Jennifer; Tamm, Alexandra; Ruckteschler, Nina; Lena Leifke, Anna; Weber, Bettina

2018-03-01

Chlorophyll concentrations of biological soil crust (biocrust) samples are commonly determined to quantify the relevance of photosynthetically active organisms within these surface soil communities. Whereas chlorophyll extraction methods for freshwater algae and leaf tissues of vascular plants are well established, there is still some uncertainty regarding the optimal extraction method for biocrusts, where organism composition is highly variable and samples comprise major amounts of soil. In this study we analyzed the efficiency of two different chlorophyll extraction solvents, the effect of grinding the soil samples prior to the extraction procedure, and the impact of shaking as an intermediate step during extraction. The analyses were conducted on four different types of biocrusts. Our results show that for all biocrust types chlorophyll contents obtained with ethanol were significantly lower than those obtained using dimethyl sulfoxide (DMSO) as a solvent. Grinding of biocrust samples prior to analysis caused a highly significant decrease in chlorophyll content for green algal lichen- and cyanolichen-dominated biocrusts, and a tendency towards lower values for moss- and algae-dominated biocrusts. Shaking of the samples after each extraction step had a significant positive effect on the chlorophyll content of green algal lichen- and cyanolichen-dominated biocrusts. Based on our results we confirm a DMSO-based chlorophyll extraction method without grinding pretreatment and suggest the addition of an intermediate shaking step for complete chlorophyll extraction (see Supplement S6 for detailed manual). Determination of a universal chlorophyll extraction method for biocrusts is essential for the inter-comparability of publications conducted across all continents.

Identifying Distribution of Chlorophyll-a Concentration Using Landsat 8 OLI on Marine Waters Area of Cirebon

Science.gov (United States)

Buditama, Gilang; Damayanti, Astrid; Giok Pin, Tjiong

2017-12-01

Phytoplankton is a microscopic plant that has a function to produces oxygen and organic substances. It also plays a role as a main producer in the food chain of a marine ecosystem. Chlorophyll-a is a colour pigment most common in phytoplankton species, so that the concentration level of chlorophyll-a can be used as an indicator of the abundance of phytoplankton cells, and as a reference for predicting organic potency in the aquatic area. This research discusses about the spatial and temporal distribution of chlorophyll-a and its correlation with salinity and total suspended solid (TSS), in the seawaters of Cirebon, West Java. The goal of this research is to be a source of information for fishermen, and other stakeholders whose related subjects in the field of marine and fisheries to predict fertile water regions and can also be used as an indicator in discovering potential areas to catch pelagic fish in Cirebon seawaters. Chlorophyll-a concentration, salinity, and TSS are identified using remote sensing data such as Landsat-8 OLI multi temporal images according to dry and wet month parameters in the 2014-2015. The results of the processed image are then validated between in-situ measurements in the field and remote sensing imagery at the same time. This research utilizes descriptive analysis, and statistics with spatial approach. The results of the research show that temporally, chlorophyll-a levels have a tendency to be higher in wet months compared to dry months, while chlorophyll-a is higher in areas near the coastline compared to open sea areas. The distribution of chlorophyll-a concentration is affected by salinity and TSS distribution, where salinity has the negative relationship and TSS has the positive relationship with chlorophyll-a distribution.

Assessment of The trophic state and Chlorophyll-a concentrations using Landsat OLI in Karaoun reservoir, Lebanon

Directory of Open Access Journals (Sweden)

Ali Fadel

2016-12-01

Full Text Available Fadel, A., Faour G. and Slim K. 2016. Assessment of the trophic state and chlorophyll-a concentrations using Landsat OLI in Karaoun reservoir, Lebanon. Lebanese Science Journal, 17(2: 130-145. Harmful algal blooms have become a worldwide environmental problem. A regular and cost-effective monitoring of these blooms is highly needed by lakes managers. Satellite remote sensing imagery like Landsat Operational Land Imager (OLI can be used to assess and monitor chlorophyll-a in water bodies over large areas in a cost-effective way. In this study, the accuracy of Landsat OLI to estimate chlorophyll-a was examined. Four field campaigns and cloud free images of Landsat OLI with 30 m resolution (01 May 2013, 21 August 2013, 10 July 2015, and 11 August 2015 were used in this study to determine the accuracy of Landsat OLI in estimating chlorophyll-a in a 12 km2 freshwater body, Karaoun reservoir. After atmospheric correction of these images, reflectance of single and multiple band combinations were compared to field chlorophyll-a data. Results of field campaigns showed that the trophic state of Karaoun reservoir is still eutrophic to hypereutrophic with high nutrient concentration and low phytoplankton biodiversity, dominated by cyanobacteria species, Microcystis aeruginosa and Aphanizomenon ovalisporum. On single band level, the in situ chlorophyll-a measurement correlated best with band 5 (0.85 - 0.88 µm, with R=0.75 and R2=0.57. Highest correlation (R=0.84 and R2=0.72 was obtained using band combination, B2:B4 band ratio multiplied by B5. Results indicated that Landsat OLI can be used effectively to determine chlorophyll-a concentration in lakes and reservoirs. We recommend the application of Landsat OLI as a satisfactory and cost effective method for monitoring chlorophyll-a in other lakes through-out the world

Relationships between nutrients and chlorophyll a concentration in the international Alma Gol Wetland, Iran

Directory of Open Access Journals (Sweden)

Saeed Balali

2013-05-01

Full Text Available This study investigated the relationships between nutrients and chlorophyll, a concentration in the International Alma Gol Wetland. Chlorophyll a is the major photosynthetic pigment in lots of phytoplanktons and has been used as a trophy index in aquatic ecosystems. Water samples were collected fortnightly from five stations in the wetland during summer and autumn. Chlorophyll-a ranged between 4.38 to 156.55 mg/m3, sulfate ranged between 138 to 190 mg/l, total alkalinity ranged between 80 to 280 mg/l, silica ranged between 3.80 to 35.00 mg/l, phosphate ranged between 0.02 to 3.70 mg/l, ammonia ranged between 0.10 to 11.90 mg/l, nitrate ranged between 0.01 to 2.75 mg/l and nitrite ranged between 0.01 to 0.39 mg/l. There was a significant correlation between chlorophyll a and nitrate, nitrite and ammonia but there was no significant correlation between chlorophyll a and silica, total alkalinity, sulfate and phosphorus.

Filtering remotely sensed chlorophyll concentrations in the Red Sea using a space-time covariance model and a Kalman filter

KAUST Repository

Dreano, Denis

2015-04-27

A statistical model is proposed to filter satellite-derived chlorophyll concentration from the Red Sea, and to predict future chlorophyll concentrations. The seasonal trend is first estimated after filling missing chlorophyll data using an Empirical Orthogonal Function (EOF)-based algorithm (Data Interpolation EOF). The anomalies are then modeled as a stationary Gaussian process. A method proposed by Gneiting (2002) is used to construct positive-definite space-time covariance models for this process. After choosing an appropriate statistical model and identifying its parameters, Kriging is applied in the space-time domain to make a one step ahead prediction of the anomalies. The latter serves as the prediction model of a reduced-order Kalman filter, which is applied to assimilate and predict future chlorophyll concentrations. The proposed method decreases the root mean square (RMS) prediction error by about 11% compared with the seasonal average.

Filtering remotely sensed chlorophyll concentrations in the Red Sea using a space-time covariance model and a Kalman filter

KAUST Repository

Dreano, Denis; Mallick, Bani; Hoteit, Ibrahim

2015-01-01

A statistical model is proposed to filter satellite-derived chlorophyll concentration from the Red Sea, and to predict future chlorophyll concentrations. The seasonal trend is first estimated after filling missing chlorophyll data using an Empirical Orthogonal Function (EOF)-based algorithm (Data Interpolation EOF). The anomalies are then modeled as a stationary Gaussian process. A method proposed by Gneiting (2002) is used to construct positive-definite space-time covariance models for this process. After choosing an appropriate statistical model and identifying its parameters, Kriging is applied in the space-time domain to make a one step ahead prediction of the anomalies. The latter serves as the prediction model of a reduced-order Kalman filter, which is applied to assimilate and predict future chlorophyll concentrations. The proposed method decreases the root mean square (RMS) prediction error by about 11% compared with the seasonal average.

Ocean Chlorophyll as a Precursor of ENSO: An Earth System Modeling Study

Science.gov (United States)

Park, Jong-Yeon; Dunne, John P.; Stock, Charles A.

2018-02-01

Ocean chlorophyll concentration, a proxy for phytoplankton, is strongly influenced by internal ocean dynamics such as those associated with El Niño-Southern Oscillation (ENSO). Observations show that ocean chlorophyll responses to ENSO generally lead sea surface temperature (SST) responses in the equatorial Pacific. A long-term global Earth system model simulation incorporating marine biogeochemical processes also exhibits a preceding chlorophyll response. In contrast to simulated SST anomalies, which significantly lag the wind-driven subsurface heat response to ENSO, chlorophyll anomalies respond rapidly. Iron was found to be the key factor connecting the simulated surface chlorophyll anomalies to the subsurface ocean response. Westerly wind bursts decrease central Pacific chlorophyll by reducing iron supply through wind-driven thermocline deepening but increase western Pacific chlorophyll by enhancing the influx of coastal iron from the maritime continent. Our results mechanistically support the potential for chlorophyll-based indices to inform seasonal ENSO forecasts beyond previously identified SST-based indices.

Cross-system comparison of factors influencing chlorophyll-a concentration in Oregon estuaries

Science.gov (United States)

Water column chlorophyll-a (chla) is a proxy for phytoplankton biomass and is often used as a biological response indicator of eutrophication. Although watershed nutrient loading may influence chla concentration in estuaries, factors such as freshwater inflow, residence time, and...

Using visible reflectance spectroscopy to reconstruct historical changes in chlorophyll a concentration in East Antarctic ponds

Directory of Open Access Journals (Sweden)

Qianqian Chen

2013-12-01

Full Text Available The visible reflectance spectroscopy (VRS and chlorophyll a concentration were determined in three sediment profiles collected from East Antarctica to investigate the potential application of VRS in reconstructing historical changes in Antarctic lake primary productivity. The results showed that the appearance of a trough at 650–700 nm is an important marker for chlorophyll a concentration and can therefore be used to distinguish the sedimentary organic matter source from guano and algae. The measured chlorophyll a content had significant positive correlations with the trough area between 650 and 700 nm, and no distinct trough was found in the sediments with organic matter completely derived from guano. Modelling results showed that the spectra spectrally inferred chlorophyll a content, and the measured data exhibit consistent trends with depth, showing that the dimensionless trough area can serve as an independent proxy for reconstructing historical fluctuations in the primary production of Antarctic ponds. The correlation of phosphorus (P with measured and inferred chlorophyll a contents in ornithogenic sediments near penguin colonies indicates that the change in primary productivity in the Antarctic ponds investigated was closely related to the amount of guano input from these birds.

Seasonal variability of sea surface chlorophyll-a of waters around ...

Indian Academy of Sciences (India)

days during 1978--1986 are processed to produce sea surface chlorophyll maps ... shallow water areas, in particular waters in Palk Bay and Gulf of Mannar, should be carried out in order .... The circulation penetrates deeper, affecting the.

The effect of different uranium concentrations on physiological characteristics and chlorophyll contents in sunflowers and soy bean

International Nuclear Information System (INIS)

Bagherifam, S.; Lakzian, A.; Ahmadi, S. J.; Fotovat, A.; Rahimi, M. F.

2009-01-01

Uranium as a natural radioactive heavy metal, widely disperses throughout the earth's crust. In many cases, the natural abundance has been re-distributed due to anthropogenic activities, resulting in radionuclide contamination in groundwater and surface soil. A pot experiment had been conducted in the Agricultural College Research Greenhouse, at the Ferdowsi University of Mashhad under the controlled condition. The effect of six levels of uranium (0, 50, 100, 250, 500 and 1000 mg U kg -1 ) on physiological characteristics and chlorophyll contents in sunflower and soy bean were studied in a completely randomized design as a factorial experiment with three replications. Plants were harvested after 40 days and before the reproductive stages. Root and stem length, root dry weight, stem dry weight, biomass and chlorophyll contents were determined. The shoot and root length, fresh and dry mass as well as leaf area and chlorophyll contents showed a significant negative correlation with the applied uranium concentrations. The influence on plant growth was also measured in terms of tolerance index and grade of growth inhibition. The results showed that tolerance index increased and grade od growth inhibition decreased with the applied uranium concentration. Biomass and tolerance of sunflower during the experiment on higher uranium concentrations showed that sun flower is more resistant against uranium toxicity

Assessment of the Trophic State and Chlorophyll-A Concentrations using Landsat OLI in Karaoun Reservoir Lebanon

International Nuclear Information System (INIS)

Fadel, A.; Faour, GH.; Slim, K.

2016-01-01

Harmful algal blooms have become a worldwide environmental problem. A regular and cost -effective monitoring of these blooms is highly needed by lakes managers. Satellite remote sensing imagery like Landsat Operational Land Imager (OLI) can be used to assess and monitor chlorophyll-a in water bodies over large areas in a cost-effective way. In this study, the accuracy of Landsat OLI to estimate chlorophyll-a was examined. Four field campaigns and cloud free images of Landsat OLI with 30 m resolution (01 May 2013, 21 August 2013, 10 July 2015, and 11 August 2015) were used in this study to determine the accuracy of Landsat OLI in estimating chlorophyll-a in a 12 km2 fresh water body, Karaoun reservoir. After atmospheric correction of these images, reflectance of single and multiple band combinations were compared to field chlorophyll-a data. Results of field campaigns showed that the trophic state of Karaoun reservoir is still eutrophic to hypereutrophic withhigh nutrient concentration andlow phytoplankton biodiversity, dominatedby cyanobacteria species, Microcystis aeruginosa and Aphanizomenon ovalisporum. On single band level, the n situ chlorophyll-a measurement correlated best with band 5 (0.85 -0.88 μm), with R=0.75 and R2=0.57.Highest correlation (R=0.84 and R2=0.72) was obtained using band combination, B2:B4 band ratio multiplied by B5. Results indicated that Landsat OLI can be used effectively to determine chlorophyll-a concentration in lakes and reservoirs. We recommend the application of Landsat OLI as a satisfactory and cost effective method for monitoring chlorophyll-a in other lakes through-out the world. (author)

Associations between chlorophyll a and various microcystin health advisory concentrations [version 2; referees: 1 approved, 2 approved with reservations

Directory of Open Access Journals (Sweden)

Jeffrey W. Hollister

2016-06-01

Full Text Available Cyanobacteria harmful algal blooms (cHABs are associated with a wide range of adverse health effects that stem mostly from the presence of cyanotoxins. To help protect against these impacts, several health advisory levels have been set for some toxins. In particular, one of the more common toxins, microcystin, has several advisory levels set for drinking water and recreational use. However, compared to other water quality measures, field measurements of microcystin are not commonly available due to cost and advanced understanding required to interpret results. Addressing these issues will take time and resources. Thus, there is utility in finding indicators of microcystin that are already widely available, can be estimated quickly and in situ, and used as a first defense against high levels of microcystin. Chlorophyll a is commonly measured, can be estimated in situ, and has been shown to be positively associated with microcystin. In this paper, we use this association to provide estimates of chlorophyll a concentrations that are indicative of a higher probability of exceeding select health advisory concentrations for microcystin. Using the 2007 National Lakes Assessment and a conditional probability approach, we identify chlorophyll a concentrations that are more likely than not to be associated with an exceedance of a microcystin health advisory level. We look at the recent US EPA health advisories for drinking water as well as the World Health Organization levels for drinking water and recreational use and identify a range of chlorophyll a thresholds. A 50% chance of exceeding one of the specific advisory microcystin concentrations of 0.3, 1, 1.6, and 2 μg/L is associated with chlorophyll a concentration thresholds of 23, 68, 84, and 104 μg/L, respectively. When managing for these various microcystin levels, exceeding these reported chlorophyll a concentrations should be a trigger for further testing and possible management action.

Spatio-temporal patterns of Ulva prolifera blooms and the corresponding influence on chlorophyll-a concentration in the Southern Yellow Sea, China.

Science.gov (United States)

Sun, Xiao; Wu, Mengquan; Xing, Qianguo; Song, Xiaodong; Zhao, Deheng; Han, Qianqian; Zhang, Guangzong

2018-06-04

The world's largest macroalgal blooms (MABs) caused by the Ulva prolifera outbreaks have occurred every summer since 2007 in the Southern Yellow Sea, China. Accumulating evidence showed that MABs may deteriorate the regional marine environment and influence the growth of some primary producers such as phytoplankton. In this study, we investigated the spatio-temporal patterns of U. prolifera green tides and chlorophyll-a concentration in the Southern Yellow Sea in 2015 using satellite images obtained from HJ-1 CCD, MODIS, and GOCI. The correlation between the distributions of U. prolifera abundance and chlorophyll-a concentration was analyzed quantitatively by setting up a series of 5 × 5 km experimental grids, and we also discussed the possible mechanisms about the influence of U. prolifera blooms on the other floating microalgae. The results showed that the development of U. prolifera blooms in the Southern Yellow Sea in 2015 could be featured as "appearance - development - outbreak - decline - disappearance", while the concentration of chlorophyll-a showed "increase - sharp decline - slow recovery - stabilization" from April to August. We also found that the concentration of chlorophyll-a had the following relationships with U. prolifera temporally: (1) the concentration of chlorophyll-a increased with the growth of U. prolifera from April to mid-May; (2) the chlorophyll-a concentration decreased sharply with the dramatically increased coverage of U. prolifera in June; and (3) the chlorophyll-a concentration slowly recovered and finally stabilized as U. prolifera decreased in July. Generally, there was a negative correlation between the occurrence of U. prolifera and chlorophyll-a concentration in the Southern Yellow Sea, China. Our results showed that the outbreak of U. prolifera does have a certain impact on the growth and reproduction of planktonic microalgae, and it suggests that U. prolifera blooms have potentially altered the ecological balance in

Thermocline Regulated Seasonal Evolution of Surface Chlorophyll in the Gulf of Aden

KAUST Repository

Yao, Fengchao; Hoteit, Ibrahim

2015-01-01

, the Sea-Viewing Wide Field-of-View Sensor (SeaWiFS) ocean color data reveal that the Gulf of Aden also exhibits a prominent summer chlorophyll bloom and sustains elevated chlorophyll concentrations throughout the fall, and is a biophysical province

Revisiting chlorophyll extraction methods in biological soil crusts – methodology for determination of chlorophyll a and chlorophyll a + b as compared to previous methods

Directory of Open Access Journals (Sweden)

J. Caesar

2018-03-01

Full Text Available Chlorophyll concentrations of biological soil crust (biocrust samples are commonly determined to quantify the relevance of photosynthetically active organisms within these surface soil communities. Whereas chlorophyll extraction methods for freshwater algae and leaf tissues of vascular plants are well established, there is still some uncertainty regarding the optimal extraction method for biocrusts, where organism composition is highly variable and samples comprise major amounts of soil. In this study we analyzed the efficiency of two different chlorophyll extraction solvents, the effect of grinding the soil samples prior to the extraction procedure, and the impact of shaking as an intermediate step during extraction. The analyses were conducted on four different types of biocrusts. Our results show that for all biocrust types chlorophyll contents obtained with ethanol were significantly lower than those obtained using dimethyl sulfoxide (DMSO as a solvent. Grinding of biocrust samples prior to analysis caused a highly significant decrease in chlorophyll content for green algal lichen- and cyanolichen-dominated biocrusts, and a tendency towards lower values for moss- and algae-dominated biocrusts. Shaking of the samples after each extraction step had a significant positive effect on the chlorophyll content of green algal lichen- and cyanolichen-dominated biocrusts. Based on our results we confirm a DMSO-based chlorophyll extraction method without grinding pretreatment and suggest the addition of an intermediate shaking step for complete chlorophyll extraction (see Supplement S6 for detailed manual. Determination of a universal chlorophyll extraction method for biocrusts is essential for the inter-comparability of publications conducted across all continents.

Energy transfer between surface-immobilized light-harvesting chlorophyll a/b complex (LHCII) studied by surface plasmon field-enhanced fluorescence spectroscopy (SPFS).

Science.gov (United States)

Lauterbach, Rolf; Liu, Jing; Knoll, Wolfgang; Paulsen, Harald

2010-11-16

The major light-harvesting chlorophyll a/b complex (LHCII) of the photosynthetic apparatus in green plants can be viewed as a protein scaffold binding and positioning a large number of pigment molecules that combines rapid and efficient excitation energy transfer with effective protection of its pigments from photobleaching. These properties make LHCII potentially interesting as a light harvester (or a model thereof) in photoelectronic applications. Most of such applications would require the LHCII to be immobilized on a solid surface. In a previous study we showed the immobilization of recombinant LHCII on functionalized gold surfaces via a 6-histidine tag (His tag) in the protein moiety. In this work the occurrence and efficiency of Förster energy transfer between immobilized LHCII on a functionalized surface have been analyzed by surface plasmon field-enhanced fluorescence spectroscopy (SPFS). A near-infrared dye was attached to some but not all of the LHC complexes, serving as an energy acceptor to chlorophylls. Analysis of the energy transfer from chlorophylls to this acceptor dye yielded information about the extent of intercomplex energy transfer between immobilized LHCII.

Decomposing the effects of ocean warming on chlorophyll a concentrations into physically and biologically driven contributions

International Nuclear Information System (INIS)

Olonscheck, D; Hofmann, M; Schellnhuber, H J; Worm, B

2013-01-01

Recently compiled observational data suggest a substantial decline in the global median chlorophyll a concentration over the 20th century, a trend that appears to be linked to ocean warming. Several modelling studies have considered changes in the ocean’s physical structure as a possible cause, while experimental work supports a biological mechanism, namely an observed increase in zooplankton grazing rate that outpaces phytoplankton production at higher temperatures. Here, we present transient simulations derived from a coupled ocean general circulation and carbon cycle model forced by atmospheric fields under unabated anthropogenic global warming (IPCC SRES A1FI scenario). The simulations account for both physical and biological mechanisms, and can reproduce about one quarter of the observed chlorophyll a decline during the 20th century, when using realistically parameterized temperature sensitivity of zooplankton metabolism (Q 10 between 2 and 4) and phytoplankton growth (Q 10 ∼ 1.9). Therefore, we have employed and re-calibrated the standard ecosystem model which assumes a lower temperature sensitivity of zooplankton grazing (Q 10 = 1.1049) by re-scaling phytoplankton growth rates and zooplankton grazing rates. Our model projects a global chlorophyll a decline of >50% by the end of the 21st century. While phytoplankton abundance and chlorophyll a experience pronounced negative effects, primary production and zooplankton concentrations are less sensitive to ocean warming. Although changes in physical structure play an important role, much of the simulated change in chlorophyll a and productivity is related to the uneven temperature sensitivity of the marine ecosystem. (letter)

Principal Component Analysis of Chlorophyll Content in Tobacco, Bean and Petunia Plants Exposed to Different Tropospheric Ozone Concentrations

Science.gov (United States)

Borowiak, Klaudia; Zbierska, Janina; Budka, Anna; Kayzer, Dariusz

2014-06-01

Three plant species were assessed in this study - ozone-sensitive and -resistant tobacco, ozone-sensitive petunia and bean. Plants were exposed to ambient air conditions for several weeks in two sites differing in tropospheric ozone concentrations in the growing season of 2009. Every week chlorophyll contents were analysed. Cumulative ozone effects on the chlorophyll content in relation to other meteorological parameters were evaluated using principal component analysis, while the relation between certain days of measurements of the plants were analysed using multivariate analysis of variance. Results revealed variability between plant species response. However, some similarities were noted. Positive relations of all chlorophyll forms to cumulative ozone concentration (AOT 40) were found for all the plant species that were examined. The chlorophyll b/a ratio revealed an opposite position to ozone concentration only in the ozone-resistant tobacco cultivar. In all the plant species the highest average chlorophyll content was noted after the 7th day of the experiment. Afterwards, the plants usually revealed various responses. Ozone-sensitive tobacco revealed decrease of chlorophyll content, and after few weeks of decline again an increase was observed. Probably, due to the accommodation for the stress factor. While during first three weeks relatively high levels of chlorophyll contents were noted in ozone-resistant tobacco. Petunia revealed a slow decrease of chlorophyll content and the lowest values at the end of the experiment. A comparison between the plant species revealed the highest level of chlorophyll contents in ozone-resistant tobacco.

Chlorophyll-a Concentration Retrieval in the Optically Complex Waters of the St. Lawrence Estuary and Gulf Using Principal Component Analysis

Directory of Open Access Journals (Sweden)

Julien Laliberté

2018-02-01

Full Text Available Empirical methods based on band ratios to infer chlorophyll-a concentration by satellite do not perform well over the optically complex waters of the St. Lawrence Estuary and Gulf. Using a dataset of 93 match-ups, we explore an alternative method relying on empirical orthogonal functions (EOF to develop an algorithm that relates the satellite-derived remote sensing reflectances to in situ chlorophyll-a concentration for the Sea-viewing Wide Field-of-view Sensor (SeaWiFS. Results show that an accuracy of 41% at retrieving chlorophyll-a concentration can be reached using the EOF method compared to 140% for the widely-used Ocean Chlorophyll 4 (OC4v4 empirical algorithm, 53% for the Garver-Siegel-Maritorena (GSM01 and 54% for the Generalized Inherent Optical Property (GIOP semi-analytical algorithms. This result is possible because the EOF approach is able to extract region-specific radiometric features from the satellite remote sensing reflectances that are related to absorption properties of optical components (water, coloured dissolved organic matter and chlorophyll-a using the visible SeaWiFS channels. The method could easily be used with other ocean-colour satellite sensors (e.g., MODIS, MERIS, VIIRS, OLCI to extend the time series for the St. Lawrence Estuary and Gulf waters.

Associations between chlorophyll a and various microcystin-LR health advisory concentrations [version 1; referees: 1 approved, 2 approved with reservations

Directory of Open Access Journals (Sweden)

Jeffrey W. Hollister

2016-02-01

Full Text Available Cyanobacteria harmful algal blooms (cHABs are associated with a wide range of adverse health effects that stem mostly from the presence of cyanotoxins. To help protect against these impacts, several health advisory levels have been set for some toxins. In particular, one of the more common toxins, microcystin-LR, has several advisory levels set for drinking water and recreational use. However, compared to other water quality measures, field measurements of microcystin-LR are not commonly available due to cost and advanced understanding required to interpret results. Addressing these issues will take time and resources. Thus, there is utility in finding indicators of microcystin-LR that are already widely available, can be estimated quickly and in situ, and used as a first defense against high concentrations of microcystin-LR. Chlorophyll a is commonly measured, can be estimated in situ, and has been shown to be positively associated with microcystin-LR. In this paper, we use this association to provide estimates of chlorophyll a concentrations that are indicative of a higher probability of exceeding select health advisory concentrations for microcystin-LR. Using the 2007 National Lakes Assessment and a conditional probability approach, we identify chlorophyll a concentrations that are more likely than not to be associated with an exceedance of a microcystin-LR health advisory level. We look at the recent US EPA health advisories for drinking water as well as the World Health Organization levels for drinking water and recreational use and identify a range of chlorophyll a thresholds. A 50% chance of exceeding one of the microcystin-LR advisory concentrations of 0.3, 1, 1.6, and 2 g/L is associated with chlorophyll a concentration thresholds of 23.4, 67.0, 83.5, and 105.8, respectively. When managing for these various microcystin-LR levels, exceeding these reported chlorophyll a concentrations should be a trigger for

Temporal variability of chlorophyll distribution in the Gulf of Mexico: bio-optical data from profiling floats

Science.gov (United States)

Pasqueron de Fommervault, Orens; Perez-Brunius, Paula; Damien, Pierre; Camacho-Ibar, Victor F.; Sheinbaum, Julio

2017-12-01

Chlorophyll concentration is a key oceanic biogeochemical variable. In the Gulf of Mexico (GOM), its distribution, which is mainly obtained from satellite surface observations and scarce in situ experiments, is still poorly understood. In 2011-2012, eight profiling floats equipped with biogeochemical sensors were deployed for the first time in the GOM and generated an unprecedented dataset that significantly increased the number of chlorophyll vertical distribution measurements in the region. The analysis of these data, once calibrated, permits us to reconsider the spatial and temporal variability of the chlorophyll concentration in the water column. At a seasonal scale, results confirm the surface signal seen by satellites, presenting maximum concentrations in winter and low values in summer. It is shown that the deepening of the mixed layer is the primary factor triggering the chlorophyll surface increase in winter. In the GOM, a possible interpretation is that this surface increase corresponds to a biomass increase. However, the present dataset suggests that the basin-scale climatological surface increase in chlorophyll content results from a vertical redistribution of subsurface chlorophyll and/or photoacclimation processes, rather than a net increase of biomass. One plausible explanation for this is the decoupling between the mixed-layer depth and the deep nutrient reservoir since mixed-layer depth only reaches the nitracline in sporadic events in the observations. Float measurements also provide evidence that the depth and the magnitude of the deep chlorophyll maximum is strongly controlled by the mesoscale variability, with higher chlorophyll biomass generally observed in cyclones rather than anticyclones.

A new three-band algorithm for estimating chlorophyll concentrations in turbid inland lakes

International Nuclear Information System (INIS)

Duan Hongtao; Ma Ronghua; Zhao Chenlu; Zhou Lin; Shang Linlin; Zhang Yuanzhi; Loiselle, Steven Arthur; Xu Jingping

2010-01-01

A new three-band model was developed to estimate chlorophyll-a concentrations in turbid inland waters. This model makes a number of important improvements with respect to the three-band model commonly used, including lower restrictions on wavelength optimization and the use of coefficients which represent specific inherent optical properties. Results showed that the new model provides a significantly higher determination coefficient and lower root mean squared error (RMSE) with respect to the original model for upwelling data from Taihu Lake, China. The new model was tested using simulated data for the MERIS and GOCI satellite systems, showing high correlations with the former and poorer correlations with the latter, principally due to the lack of a 709 nm centered waveband. The new model provides numerous advantages, making it a suitable alternative for chlorophyll-a estimations in turbid and eutrophic waters.

Simultaneous Measurements of Chlorophyll Concentration by Lidar, Fluorometry, above-Water Radiometry, and Ocean Color MODIS Images in the Southwestern Atlantic

Directory of Open Access Journals (Sweden)

Cristina M. Bentz

2009-01-01

Full Text Available Comparisons between in situ measurements of surface chlorophyll-a concentration (CHL and ocean color remote sensing estimates were conducted during an oceanographic cruise on the Brazilian Southeastern continental shelf and slope, Southwestern South Atlantic. In situ values were based on fluorometry, above-water radiometry and lidar fluorosensor. Three empirical algorithms were used to estimate CHL from radiometric measurements: Ocean Chlorophyll 3 bands (OC3MRAD, Ocean Chlorophyll 4 bands (OC4v4RAD, and Ocean Chlorophyll 2 bands (OC2v4RAD. The satellite estimates of CHL were derived from data collected by the MODerate-resolution Imaging Spectroradiometer (MODIS with a nominal 1.1 km resolution at nadir. Three algorithms were used to estimate chlorophyll concentrations from MODIS data: one empirical - OC3MSAT, and two semi-analytical - Garver, Siegel, Maritorena version 01 (GSM01SAT, and CarderSAT. In the present work, MODIS, lidar and in situ above-water radiometry and fluorometry are briefly described and the estimated values of chlorophyll retrieved by these techniques are compared. The chlorophyll concentration in the study area was in the range 0.01 to 0.2 mg·m-3. In general, the empirical algorithms applied to the in situ radiometric and satellite data showed a tendency to overestimate CHL with a mean difference between estimated and measured values of as much as 0.17 mg/m3 (OC2v4RAD. The semi-analytical GSM01 algorithm applied to MODIS data performed better (rmse 0.28, rmse-L 0.08, mean diff. -0.01 mg/m3 than the Carder and the empirical OC3M algorithms (rmse 1.14 and 0.36, rmse-L 0.34 and 0.11, mean diff. 0.17 and 0.02 mg/m3, respectively. We find that rmsd values between MODIS relative to the in situ radiometric measurements are < 26%, i.e., there is a trend towards overestimation of RRS by MODIS for the stations considered in this work. Other authors have already reported over and under estimation of MODIS remotely sensed

Simultaneous Measurements of Chlorophyll Concentration by Lidar, Fluorometry, above-Water Radiometry, and Ocean Color MODIS Images in the Southwestern Atlantic.

Science.gov (United States)

Kampel, Milton; Lorenzzetti, João A; Bentz, Cristina M; Nunes, Raul A; Paranhos, Rodolfo; Rudorff, Frederico M; Politano, Alexandre T

2009-01-01

Comparisons between in situ measurements of surface chlorophyll-a concentration (CHL) and ocean color remote sensing estimates were conducted during an oceanographic cruise on the Brazilian Southeastern continental shelf and slope, Southwestern South Atlantic. In situ values were based on fluorometry, above-water radiometry and lidar fluorosensor. Three empirical algorithms were used to estimate CHL from radiometric measurements: Ocean Chlorophyll 3 bands (OC3M(RAD)), Ocean Chlorophyll 4 bands (OC4v4(RAD)), and Ocean Chlorophyll 2 bands (OC2v4(RAD)). The satellite estimates of CHL were derived from data collected by the MODerate-resolution Imaging Spectroradiometer (MODIS) with a nominal 1.1 km resolution at nadir. Three algorithms were used to estimate chlorophyll concentrations from MODIS data: one empirical - OC3M(SAT), and two semi-analytical - Garver, Siegel, Maritorena version 01 (GSM01(SAT)), and Carder(SAT). In the present work, MODIS, lidar and in situ above-water radiometry and fluorometry are briefly described and the estimated values of chlorophyll retrieved by these techniques are compared. The chlorophyll concentration in the study area was in the range 0.01 to 0.2 mg/m(3). In general, the empirical algorithms applied to the in situ radiometric and satellite data showed a tendency to overestimate CHL with a mean difference between estimated and measured values of as much as 0.17 mg/m(3) (OC2v4(RAD)). The semi-analytical GSM01 algorithm applied to MODIS data performed better (rmse 0.28, rmse-L 0.08, mean diff. -0.01 mg/m(3)) than the Carder and the empirical OC3M algorithms (rmse 1.14 and 0.36, rmse-L 0.34 and 0.11, mean diff. 0.17 and 0.02 mg/m(3), respectively). We find that rmsd values between MODIS relative to the in situ radiometric measurements are MODIS for the stations considered in this work. Other authors have already reported over and under estimation of MODIS remotely sensed reflectance due to several errors in the bio-optical algorithm

PIXE analysis of trace elements in relation to chlorophyll concentration in Plantago ovata Forsk

International Nuclear Information System (INIS)

Saha, Priyanka; Sen Raychaudhuri, Sarmistha; Chakraborty, Anindita; Sudarshan, Mathummal

2010-01-01

Plantago ovata Forsk - an economically important medicinal plant - was analyzed for trace elements and chlorophyll in a study of the effects of gamma radiation on physiological responses of the seedlings. Proton-induced X-ray emission (PIXE) technique was used to quantify trace elements in unirradiated and gamma-irradiated plants at the seedling stage. The experiments revealed radiation-induced changes in the trace element and chlorophyll concentrations.

Relationships between Concentrations of Phytoplankton Chlorophyll a and Total Nitrogen in Ten U.S. Estuaries

Science.gov (United States)

This presentation focuses on the summertime response of phytoplankton chlorophyll to nitrogen concentrations in the upper water columns of ten U.S. estuaries. Using publicly available data from monitoring programs, regression relationships have been developed between summer surfa...

Seasonality in sub-surface chlorophyll maxima in the Arabian Sea: Detection by IRS-P4/OCM and implication of it to primary productivity

Digital Repository Service at National Institute of Oceanography (India)

Matondkar, S.G.P.; Parab, S.G.; Dwivedi, R.M.

various seasons. During November at St. 1 surface chlorophyll a was 1.503 mgm-3and subsurface chlorophyll maxima was 12.692 mgm-3. Similarly, at St. 13 surface chlorophyll a was 0.584 mgm-3and surface chlorophyll maxima was 8.517 mgm-3. During upwelling...

Concentration Effect on Quenching of Chlorophyll a Fluorescence by All-Trans-β-Carotene in Photosynthesis

Directory of Open Access Journals (Sweden)

Chen Chen

2017-09-01

Full Text Available Absorption, fluorescence spectra of chlorophyll a (Chl-a and all-trans-β-carotene (β-Car mixing solution are investigated in different polarity and polarizability solvents. The carotenoids regulate the energy flow in photosynthesis by interaction with chlorophyll, leading to an observable reduction of Chl-a fluorescence. The fluorescence red shifts with the increasing solvent polarizability. The energy transfer in the Chl-a and β-Car system is proposed. The electron transfer should be dominant in quenching Chl-a fluorescence rather than the energy transfer in this system. Polar solvent with large polarizability shows high quenching efficiency. When dissolved in carbon tetrachloride, Chl-a presents red shift of absorption and blue shift of fluorescence spectra with increasing β-Car concentration, which implies a Chl-a conformational change.

Role of physical forcings and nutrient availability on the control of satellite-based chlorophyll a concentration in the coastal upwelling area of the Sicilian Channel

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Bernardo Patti

2010-08-01

Full Text Available The northern sector of the Sicilian Channel is an area of favourable upwelling winds, which ought to support primary production. However, the values for primary production are low when compared with other Mediterranean areas and very low compared with the most biologically productive regions of the world’s oceans: California, the Canary Islands, Humboldt and Benguela. The aim of this study was to identify the main factors that limit phytoplankton biomass in the Sicilian Channel and modulate its monthly changes. We compared satellite-based estimates of chlorophyll a concentration in the Strait of Sicily with those observed in the four Eastern Boundary Upwelling Systems mentioned above and in other Mediterranean wind-induced coastal upwelling systems (the Alboran Sea, the Gulf of Lions and the Aegean Sea. Our results show that this low level of chlorophyll is mainly due to the low nutrient level in surface and sub-surface waters, independently of wind-induced upwelling intensity. Further, monthly changes in chlorophyll are mainly driven by the mixing of water column and wind-induced and/or circulation-related upwelling processes. Finally, primary production limitation due to the enhanced stratification processes resulting from the general warming trend of Mediterranean waters is not active over most of the coastal upwelling area off the southern Sicilian coast.

Relationship between chlorophyll a concentration, light attenuation and diving depth of the Southern elephant seal Mirounga leonina.

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Thomas Jaud

Full Text Available Recently, a number of Antarctic marine environmental studies have used oceanographic parameters collected from instrumented top predators for ecological and physical information. Phytoplankton concentration is generally quantified through active measurement of chlorophyll fluorescence. In this study, light absorption coefficient (K(0.75 was used as an indicator of phytoplankton concentration. This measurement, easy to obtain and requiring low electric power, allows for assessing of the fine scale horizontal structuring of phytoplankton. As part of this study, Southern elephant seals (SES were simultaneously equipped with a fluorometer and a light logger. Along the SES tracks, variations in K(0.75 were strongly correlated with chlorophyll, a concentration measured by the fluorometer within the euphotic layer. With regards to SES foraging behaviour, bottom depth of the seal's dive was highly dependent on light intensity at 150 m, indicating that the vertical distribution of SES's prey such as myctophids is tightly related to light level. Therefore, change in phytoplankton concentration may not only have a direct effect on SES's prey abundance but may also determine their vertical accessibility with likely consequences on SES foraging efficiency.

A review of ocean chlorophyll algorithms and primary production models

Science.gov (United States)

Li, Jingwen; Zhou, Song; Lv, Nan

2015-12-01

This paper mainly introduces the five ocean chlorophyll concentration inversion algorithm and 3 main models for computing ocean primary production based on ocean chlorophyll concentration. Through the comparison of five ocean chlorophyll inversion algorithm, sums up the advantages and disadvantages of these algorithm,and briefly analyzes the trend of ocean primary production model.

Hydrography and chlorophyll a in a transect from the coast to the shelf-break in the Argentinian Sea

Science.gov (United States)

Carreto, JoséI.; Lutz, Vivian A.; Carignan, Marco O.; Cucchi Colleoni, Angel D.; De Marco, Silvia G.

1995-03-01

On the basis of data obtained during eight research cruises from a section of the Buenos Aires province shelf, three systems are characterized: (1) coastal system; (2) subantarctic shelf waters system; and (3) Malvinas system. These domains are frequently separated by two quasipermanent fronts: (1) the coastal front; and (2) the shelf-break front. The patterns of seasonal variation of phytoplankton biomass in these systems are strongly related to changes in vertical stability, that controls the availability of nutrients and light energy. In the coastal system, the mixing produced by winds and tides gives homogenenous waters all year round, having the lowest nitrate concentration and the lowest N:P ratio. The amplitude of the seasonal variation of chlorophyll was relatively small, although the highest concetrations were detected in spring and autumn. The subantarctic shelf waters system is characterized by the typical development and breakdown cycle of the seasonal thermocline. Two well defined chlorophyll a maxima are observed: the main peak during spring and the secondary one during autumn. Geographical differences occur in the timing of the development and breakdown of the thermocline. Another factor of variability is the advection of low salinity waters from the coastal region of the Rio de la Plata during spring. During winter, when nutrient concentration is the highest, an increasing nitrate gradient from the coastal front to the shelf-break region is observed. During summer, surface nitrate concentration is low over the whole continental shelf, and the highest chlorophyll a concentrations are associated with the depth of the pycnocline. However, associated with the surface nitrate maximum, chlorophyll a values higher than 2 mg m -3 are recorded at the shelf-break front. The observed surface maxima are thought to be related to physical processes associated with the slope, where the enhanced mixing results in nutrient renewal and subsequent phytoplankton growth

Optimizing the bio-optical algorithm for estimating chlorophyll-a and phycocyanin concentrations in inland waters in Korea

Science.gov (United States)

Several bio-optical algorithms were developed to estimate the chlorophyll-a (Chl-a) and phycocyanin (PC) concentrations in inland waters. This study aimed at identifying the influence of the algorithm parameters and wavelength bands on output variables and searching optimal parameter values. The opt...

The distribution feature of size-fractionated chlorophyll a and primary productivity in Prydz Bay and its north sea area during the austral summer

Institute of Scientific and Technical Information of China (English)

刘子琳; 陈忠元

2003-01-01

The investigation of size-fractionated chlorophyll a and primary productivity were carried out in three longitudinal sections (63°-69°12′S, 70°30′E, 73°E and 75(30′E) at December 18 -26, 1998 and January 12 -18, 1999 in Prydz Bay and its north sea area, Antarctica. The results showed that surface chlorophyll a concentration were 0.16 - 3.99 μg dm -3. The high values of chlorophyll a concentration ( more than 3.5 μg dm -3 ) were in Prydz Bay and in the west Ladies Bank. The average chlorophyll a concentration at sub-surface layer was higher than that at surface layer; its concentration at the deeper layers of 50 m decreased with increasing depth and that at 200 m depth was only 0.01 -0.95 μg dm-3. The results of size-fractionated chlorophyll a showed that the contribution of the netplanktion to total chlorophyll a was 56% , those of the nanoplankton and the picoplankton were 24% and 20% respectively in the surveyed area. The potential primary productivity at the euphotic zone in the surveyed area was 0. 11 - 11.67 mgC m-3 h -1 and average value was 2.00 ±2.80 mgC m-3h-1. The in-situ productivity in the bay and the continental shelf was higher and that in the deep-sea area was lower. The assimilation number of ted primary productivity show that the contribution of the netplanktion to total productivity was 58% , those of the nanoplankton and the picoplankton were 26% and 16% respectively. The cell abundance of phytoplankton was 1. 6 + 103 - 164. 8 + 103 cell dm-3 in the surface water.

Enzyme-assisted extraction of stabilized chlorophyll from spinach.

Science.gov (United States)

Özkan, Gülay; Ersus Bilek, Seda

2015-06-01

Zinc complex formation with chlorophyll derivatives in spinach pulp was studied by adding 300ppm Zn(2+) for production of stable food colorant, followed by the heating at 110°C for 15min. Zinc complex formation increased at pH values of 7.0 or greater. Pectinex Ultra SP-L was selected for enzyme-assisted release of zinc-chlorophyll derivatives from spinach pulp. Effect of enzyme concentration (1-9%), treatment temperature (30-60°C), and time (30-210min) on total chlorophyll content (TCC) were optimized using response surface methodology. A quadratic regression model (R(2)=0.9486) was obtained from the experimental design. Optimum treatment conditions were 8% enzyme concentration, 45°C, and 30min, which yielded a 50.747mgTCC/100g spinach pulp. Enzymatic treatment was followed by solvent extraction with ethanol at a solvent-to-sample ratio of 2.5:1 at 60°C for 45min for the highest TCC recovery. Pretreatment with enzyme and extraction in ethanol resulted in 39% increase in Zn-chlorophyll derivative yield. Copyright © 2014 Elsevier Ltd. All rights reserved.

SEASONAL DIFFERENCES IN SPATIAL SCALES OF CHLOROPHYLL-A CONCENTRATION IN LAKE TAIHU，CHINA

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Y. Bao

2012-08-01

Full Text Available Spatial distribution of chlorophyll-a (chla concentration in Lake Taihu is non-uniform and seasonal variability. Chla concentration retrieval algorithms were separately established using measured data and remote sensing images (HJ-1 CCD and MODIS data in October 2010, March 2011, and September 2011. Then parameters of semi- variance were calculated on the scale of 30m, 250m and 500m for analyzing spatial heterogeneity in different seasons. Finally, based on the definitions of Lumped chla (chlaL and Distributed chla (chlaD, seasonal model of chla concentration scale error was built. The results indicated that: spatial distribution of chla concentration in spring was more uniform. In summer and autumn, chla concentration in the north of the lake such as Meiliang Bay and Zhushan Bay was higher than that in the south of Lake Taihu. Chla concentration on different scales showed the similar structure in the same season, while it had different structure in different seasons. And inversion chla concentration from MODIS 500m had a greater scale error. The spatial scale error changed with seasons. It was higher in summer and autumn than that in spring. The maximum relative error can achieve 23%.

Arctic Ocean CO2 uptake: an improved multiyear estimate of the air-sea CO2 flux incorporating chlorophyll a concentrations

Science.gov (United States)

Yasunaka, Sayaka; Siswanto, Eko; Olsen, Are; Hoppema, Mario; Watanabe, Eiji; Fransson, Agneta; Chierici, Melissa; Murata, Akihiko; Lauvset, Siv K.; Wanninkhof, Rik; Takahashi, Taro; Kosugi, Naohiro; Omar, Abdirahman M.; van Heuven, Steven; Mathis, Jeremy T.

2018-03-01

We estimated monthly air-sea CO2 fluxes in the Arctic Ocean and its adjacent seas north of 60° N from 1997 to 2014. This was done by mapping partial pressure of CO2 in the surface water (pCO2w) using a self-organizing map (SOM) technique incorporating chlorophyll a concentration (Chl a), sea surface temperature, sea surface salinity, sea ice concentration, atmospheric CO2 mixing ratio, and geographical position. We applied new algorithms for extracting Chl a from satellite remote sensing reflectance with close examination of uncertainty of the obtained Chl a values. The overall relationship between pCO2w and Chl a was negative, whereas the relationship varied among seasons and regions. The addition of Chl a as a parameter in the SOM process enabled us to improve the estimate of pCO2w, particularly via better representation of its decline in spring, which resulted from biologically mediated pCO2w reduction. As a result of the inclusion of Chl a, the uncertainty in the CO2 flux estimate was reduced, with a net annual Arctic Ocean CO2 uptake of 180 ± 130 Tg C yr-1. Seasonal to interannual variation in the CO2 influx was also calculated.

Thermocline Regulated Seasonal Evolution of Surface Chlorophyll in the Gulf of Aden

KAUST Repository

Hoteit, Ibrahim; Yao, Fengchao

2015-01-01

The Gulf of Aden, although subject to seasonally reversing monsoonal winds, has been previously reported as an oligotrophic basin during summer, with elevated chlorophyll concentrations only occurring during winter due to convective mixing. However

Quantifying mangrove chlorophyll from high spatial resolution imagery

NARCIS (Netherlands)

Heenkenda, M.K.; Joyce, K.E.; Maier, S.W.; Bruin, de S.

2015-01-01

Lower than expected chlorophyll concentration of a plant can directly limit photosynthetic activity, and resultant primary production. Low chlorophyll concentration may also indicate plant physiological stress. Compared to other terrestrial vegetation, mangrove chlorophyll variations are poorly

An extended PROSPECT: Advance in the leaf optical properties model separating total chlorophylls into chlorophyll a and b.

Science.gov (United States)

Zhang, Yao; Huang, Jingfeng; Wang, Fumin; Blackburn, George Alan; Zhang, Hankui K; Wang, Xiuzhen; Wei, Chuanwen; Zhang, Kangyu; Wei, Chen

2017-07-25

The PROSPECT leaf optical model has, to date, well-separated the effects of total chlorophyll and carotenoids on leaf reflectance and transmittance in the 400-800 nm. Considering variations in chlorophyll a:b ratio with leaf age and physiological stress, a further separation of total plant-based chlorophylls into chlorophyll a and chlorophyll b is necessary for advanced monitoring of plant growth. In this study, we present an extended version of PROSPECT model (hereafter referred to as PROSPECT-MP) that can combine the effects of chlorophyll a, chlorophyll b and carotenoids on leaf directional hemispherical reflectance and transmittance (DHR and DHT) in the 400-800 nm. The LOPEX93 dataset was used to evaluate the capabilities of PROSPECT-MP for spectra modelling and pigment retrieval. The results show that PROSPECT-MP can both simultaneously retrieve leaf chlorophyll a and b, and also performs better than PROSPECT-5 in retrieving carotenoids concentrations. As for the simulation of DHR and DHT, the performances of PROSPECT-MP are similar to that of PROSPECT-5. This study demonstrates the potential of PROSPECT-MP for improving capabilities of remote sensing of leaf photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids) and for providing a framework for future refinements in the modelling of leaf optical properties.

Assessing spatial and temporal variability in phytoplankton concentration through chlorophyll-a satellite data: a case study of northern arabian sea

International Nuclear Information System (INIS)

Khan, I.A.; Ghazal, L.

2015-01-01

This study focuses on applying remote sensing technology to identify and assess seasonal and intra-annual variation of phytoplankton availability. A standard MODIS algorithm for Chlorophyll-a, is used to obtain a variation of phytoplankton with the help of MODIS time series images from April 2011 to March 2012 that describe the situation for a whole year, we also used periodical data for each three months, i.e., from April 2011 to June 2011, July 2011 to September 2011, October 2011 to December 2011 and finally January 2012 to March 2012. Chlorophyll-a (Chl-a), products were retrieved from the sensor data that demonstrates the spatio-temporal variability of phytoplankton concentration in the northern Arabian sea near the coastline and open sea water of Pakistan, India, Iran and Oman. High concentration of Chl-a, were observed during two periods August to September and February to March respectively. It was also revealed that Chl-a, concentration was almost identical between the latitude 20 and 21 degrees N throughout the year. (author)

Chlorophyll-a specific volume scattering function of phytoplankton.

Science.gov (United States)

Tan, Hiroyuki; Oishi, Tomohiko; Tanaka, Akihiko; Doerffer, Roland; Tan, Yasuhiro

2017-06-12

Chlorophyll-a specific light volume scattering functions (VSFs) by cultured phytoplankton in visible spectrum range is presented. Chlorophyll-a specific VSFs were determined based on the linear least squares method using a measured VSFs with different chlorophyll-a concentrations. We found obvious variability of it in terms of spectral and angular shapes of VSF between cultures. It was also presented that chlorophyll-a specific scattering significantly affected on spectral variation of the remote sensing reflectance, depending on spectral shape of b. This result is useful for developing an advance algorithm of ocean color remote sensing and for deep understanding of light in the sea.

Trends in Sea Ice Cover, Sea Surface Temperature, and Chlorophyll Biomass Across a Marine Distributed Biological Observatory in the Pacific Arctic Region

Science.gov (United States)

Frey, K. E.; Grebmeier, J. M.; Cooper, L. W.; Wood, C.; Panday, P. K.

2011-12-01

ice breakup and later sea ice formation. Sea surface temperatures have also shown warming, where sites show significant warming particularly during August, September, and October. Satellite-derived chlorophyll-a concentrations over the past decade have shown trends seemingly in direct response to changing sea ice conditions, with increasing trends in chlorophyll-a concentrations when sea ice declines (and vice versa). In some cases, however, satellite-derived chlorophyll-a concentrations do not show expected changes with sea ice variability, indicating that limitations on biological productivity in this region are complex and spatially heterogeneous. An understanding of these spatial and temporal complexities impacting biological productivity is needed for the accurate prediction of how overall ecosystems may be altered with further expected warming sea surface temperatures and declines in sea ice cover.

Identification and determination of flavonoids, carotenoids and chlorophyll concentration in Cynodon dactylon (L.) by HPLC analysis.

Science.gov (United States)

Muthukrishnan, Saradha Devi; Kaliyaperumal, Ashokkumar; Subramaniyan, Annapoorani

2015-01-01

Cynodon dactylon (L.) is a potent medicinal plant in the traditional and current Indian medicinal systems. The objective of this research was to find out the levels of flavonoids, carotenoids and chlorophyll b in C. dactylon leaves by high-performance liquid chromatography (HPLC) equipped with a diode array detector. HPLC analysis revealed that total carotenoid and total flavonoid concentration were 62 mg/100 g and 249.1 μg/g, respectively. The mean chlorophyll b was 85.1 mg/100 g in C. dactylon. Among the flavonoids, quercetin (164.7 μg/g) was the major flavonoid followed by kaempferol (48.2 μg/g), rutin (18.4 μg/g), catechin (12.1 μg/g) and myricetin (5.7 μg/g). Of the carotenoids, β-carotene (35.2 mg/100 g) was predominant followed by lutein (17.0 mg/100 g), violaxanthin (5.8 mg/100 g) and zeaxanthin (4.2 mg/100 g). Chlorophyll b concentration was 85.1 mg/100 g in C. dactylon. The results of this investigation should be useful information for further pharmacological studies.

Phytoplankton chlorophyll a biomass, composition, and productivity along a temperature and stratification gradient in the northeast Atlantic Ocean

NARCIS (Netherlands)

van de Poll, W. H.; Kulk, G.; Timmermans, K. R.; Brussaard, C. P. D.; van der Woerd, H. J.; Kehoe, M. J.; Mojica, K. D. A.; Visser, R. J. W.; Rozema, P. D.; Buma, A. G. J.

2013-01-01

Relationships between sea surface temperature (SST, > 10 m) and vertical density stratification, nutrient concentrations, and phytoplankton biomass, composition, and chlorophyll a (Chl a) specific absorption were assessed in spring and summer from latitudes 29 to 63 degrees N in the northeast

Chlorophyll a Covalently Bonded to Organo-Modified Translucent Silica Xerogels: Optimizing Fluorescence and Maximum Loading.

Science.gov (United States)

García-Sánchez, M A; Serratos, I N; Sosa, R; Tapia-Esquivel, T; González-García, F; Rojas-González, F; Tello-Solís, S R; Palacios-Enriquez, A Y; Esparza Schulz, J M; Arrieta, A

2016-07-22

Chlorophyll is a pyrrolic pigment with important optical properties, which is the reason it has been studied for many years. Recently, interest has been rising with respect to this molecule because of its outstanding physicochemical properties, particularly applicable to the design and development of luminescent materials, hybrid sensor systems, and photodynamic therapy devices for the treatment of cancer cells and bacteria. More recently, our research group has been finding evidence for the possibility of preserving these important properties of substrates containing chlorophyll covalently incorporated within solid pore matrices, such as SiO₂, TiO₂ or ZrO₂ synthesized through the sol-gel process. In this work, we study the optical properties of silica xerogels organo-modified on their surface with allyl and phenyl groups and containing different concentrations of chlorophyll bonded to the pore walls, in order to optimize the fluorescence that these macrocyclic species displays in solution. The intention of this investigation was to determine the maximum chlorophyll a concentration at which this molecule can be trapped inside the pores of a given xerogel and to ascertain if this pigment remains trapped as a monomer, a dimer, or aggregate. Allyl and phenyl groups were deposited on the surface of xerogels in view of their important effects on the stability of the molecule, as well as over the fluorescence emission of chlorophyll; however, these organic groups allow the trapping of either chlorophyll a monomers or dimers. The determination of the above parameters allows finding the most adequate systems for subsequent in vitro or in vivo studies. The characterization of the obtained xerogels was performed through spectroscopic absorption, emission and excitation spectra. These hybrid systems can be employed as mimics of natural systems; the entrapment of chlorophyll inside pore matrices indicates that it is possible to exploit some of the most physicochemical

Chlorophyll a Covalently Bonded to Organo-Modified Translucent Silica Xerogels: Optimizing Fluorescence and Maximum Loading

Directory of Open Access Journals (Sweden)

M. A. García-Sánchez

2016-07-01

Full Text Available Chlorophyll is a pyrrolic pigment with important optical properties, which is the reason it has been studied for many years. Recently, interest has been rising with respect to this molecule because of its outstanding physicochemical properties, particularly applicable to the design and development of luminescent materials, hybrid sensor systems, and photodynamic therapy devices for the treatment of cancer cells and bacteria. More recently, our research group has been finding evidence for the possibility of preserving these important properties of substrates containing chlorophyll covalently incorporated within solid pore matrices, such as SiO2, TiO2 or ZrO2 synthesized through the sol-gel process. In this work, we study the optical properties of silica xerogels organo-modified on their surface with allyl and phenyl groups and containing different concentrations of chlorophyll bonded to the pore walls, in order to optimize the fluorescence that these macrocyclic species displays in solution. The intention of this investigation was to determine the maximum chlorophyll a concentration at which this molecule can be trapped inside the pores of a given xerogel and to ascertain if this pigment remains trapped as a monomer, a dimer, or aggregate. Allyl and phenyl groups were deposited on the surface of xerogels in view of their important effects on the stability of the molecule, as well as over the fluorescence emission of chlorophyll; however, these organic groups allow the trapping of either chlorophyll a monomers or dimers. The determination of the above parameters allows finding the most adequate systems for subsequent in vitro or in vivo studies. The characterization of the obtained xerogels was performed through spectroscopic absorption, emission and excitation spectra. These hybrid systems can be employed as mimics of natural systems; the entrapment of chlorophyll inside pore matrices indicates that it is possible to exploit some of the most

Phytoplankton chlorophyll a biomass, composition, and productivity along a temperature and stratification gradient in the northeast Atlantic Ocean

NARCIS (Netherlands)

van de Poll, W.H.; Kulk, G.; Timmermans, K.R.; Brussaard, C.P.D.; van der Woerd, H.J.; Kehoe, M.J.; Mojica, K.D.A.; Visser, R.J.W.; Rozema, P.D.; Buma, A.G.J.

2013-01-01

Relationships between sea surface temperature (SST, > 10 m) and vertical density stratification, nutrient concentrations, and phytoplankton biomass, composition, and chlorophyll a (Chl a) specific absorption were assessed in spring and summer from latitudes 29 to 63° N in the northeast Atlantic

Indicators: Chlorophyll a

Science.gov (United States)

Chlorophyll allows plants (including algae) to photosynthesize, i.e., use sunlight to convert simple molecules into organic compounds. Chlorophyll a is the predominant type of chlorophyll found in green plants and algae.

Towards a merged satellite and in situ fluorescence ocean chlorophyll product

Directory of Open Access Journals (Sweden)

H. Lavigne

2012-06-01

Full Text Available Understanding the ocean carbon cycle requires a precise assessment of phytoplankton biomass in the oceans. In terms of numbers of observations, satellite data represent the largest available data set. However, as they are limited to surface waters, they have to be merged with in situ observations. Amongst the in situ data, fluorescence profiles constitute the greatest data set available, because fluorometers have operated routinely on oceanographic cruises since the 1970s. Nevertheless, fluorescence is only a proxy of the total chlorophyll a concentration and a data calibration is required. Calibration issues are, however, sources of uncertainty, and they have prevented a systematic and wide range exploitation of the fluorescence data set. In particular, very few attempts to standardize the fluorescence databases have been made. Consequently, merged estimations with other data sources (e.g. satellite are lacking.

We propose a merging method to fill this gap. It consists firstly in adjusting the fluorescence profile to impose a zero chlorophyll a concentration at depth. Secondly, each point of the fluorescence profile is then multiplied by a correction coefficient, which forces the chlorophyll a integrated content measured on the fluorescence profile to be consistent with the concomitant ocean colour observation. The method is close to the approach proposed by Boss et al. (2008 to correct fluorescence data of a profiling float, although important differences do exist. To develop and test our approach, in situ data from three open ocean stations (BATS, HOT and DYFAMED were used. Comparison of the so-called "satellite-corrected" fluorescence profiles with concomitant bottle-derived estimations of chlorophyll a concentration was performed to evaluate the final error (estimated at 31%. Comparison with the Boss et al. (2008 method, using a subset of the DYFAMED data set, demonstrated that the methods have similar

Synoptic-scale analysis of mechanisms driving surface chlorophyll dynamics in the North Atlantic

Directory of Open Access Journals (Sweden)

A. S. A. Ferreira

2015-06-01

Full Text Available Several hypotheses have been proposed for the onset of the spring phytoplankton bloom in the North Atlantic. Our main objective is to examine which bottom-up processes can best predict the annual increase in surface phytoplankton concentration in the North Atlantic by applying novel phenology algorithms to ocean colour data. We construct indicator fields and time series which, in various combinations, provide models consistent with the principle dynamics previously proposed. Using a multimodel inference approach, we investigate the evidence supporting these models and how it varies in space. We show that, in terms of bottom-up processes alone, there is a dominant physical mechanism, namely mixed-layer shoaling, that best predicts the interannual variation in the initial increase in surface chlorophyll across large sectors of the North Atlantic. We further show that different regions are governed by different physical phenomena and that wind-driven mixing is a common component, with either heat flux or light as triggers. We believe these findings to be relevant to the ongoing discussion on North Atlantic bloom onset.

Culture of a high-chlorophyll-producing and halotolerant Chlorella vulgaris.

Science.gov (United States)

Nakanishi, Koichi; Deuchi, Keiji

2014-05-01

In order to increase the value of freshwater algae as raw ingredients for health foods and feed for seawater-based farmed fish, we sought to breed high-chlorophyll halotolerant Chlorella with the objective of generating strains with both high chlorophyll concentrations (≥ 5%) and halotolerance (up to 1% NaCl). We used the Chlorella vulgaris K strain in our research institute culture collection and induced mutations with UV irradiation and acriflavine which is known to effect mutations of mitochondrial DNA that are associated with chlorophyll production. Screenings were conducted on seawater-based "For Chlorella spp." (FC) agar medium, and dark-green-colored colonies were visually selected by macroscopic inspection. We obtained a high-chlorophyll halotolerant strain (designated C. vulgaris M-207A7) that had a chlorophyll concentration of 6.7% (d.m.), a level at least three-fold higher than that of K strain. This isolate also exhibited a greater survival rate in seawater that of K strain. Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Synoptic-scale analysis of mechanisms driving surface chlorophyll dynamics in the North Atlantic

DEFF Research Database (Denmark)

Ferreira, Ana Sofia; Hatun, H.; Counillon, F.

2015-01-01

show that, in terms of bottom-up processes alone, there is a dominant physical mechanism, namely mixed-layer shoaling, that best predicts the interannual variation in the initial increase in surface chlorophyll across large sectors of the North Atlantic. We further show that different regions...

A bio-optical algorithm for the remote estimation of the chlorophyll-a concentration in case 2 waters

International Nuclear Information System (INIS)

Gitelson, Anatoly A; Gurlin, Daniela; Moses, Wesley J; Barrow, Tadd

2009-01-01

The objective of this work was to test the performance of a recently developed three-band model and its special case, a two-band model, for the remote estimation of the chlorophyll- a (chl-a) concentration in turbid productive case 2 waters. We specifically focused on (a) determining the ability of the models to estimate chl- a -3 , typical for coastal and estuarine waters, and (b) assessing the potential of MODIS and MERIS to estimate chl-a concentrations in turbid productive waters, using red and near-infrared (NIR) bands. Reflectance spectra and water samples were collected in 89 stations over lakes in the United States with a wide variability in optical parameters (i.e. 2.1 -3 ; 0.5 -1 ). The three-band model, using wavebands around 670, 710 and 750 nm, explains more than 89% of the chl- a variation for chl- a ranging from 2 to 20 mg m -3 and can be used to estimate chlorophyll-a concentrations with a root mean square error (RMSE) of -3 . MODIS (bands 13 and 15) and MERIS (bands 7, 9, and 10) red and NIR reflectances were simulated from the collected reflectance spectra and potential estimation errors were assessed. The MODIS two-band model is able to estimate chl- a concentrations with a RMSE of -3 for chl-a ranging from 2 to 50 mg m -3 ; however, the model loses its sensitivity for chl- a -3 . Benefiting from the higher spectral resolution of the MERIS data, the MERIS three-band model accounts for 93% of chl- a variation and is able to estimate chl-a concentrations with a RMSE of -3 for chl-a ranging from 2 to 50 mg m -3 , and a RMSE of -3 for chl-a ranging from 2 to 20 mg m -3 . These findings imply that, provided that an atmospheric correction scheme specific to the red and NIR spectral region is available, the extensive database of MODIS and MERIS images could be used to quantitatively monitor chl- a in case 2 waters.

The Use of a Chlorophyll Meter (SPAD-502) for Field Determinations of Red Mangrove (Rhizophora Mangle L.) Leaf Chlorophyll Amount

Science.gov (United States)

Connelly, Xana M.

1997-01-01

The red mangrove Rhizophora mangle L., is a halophytic woody spermatophyte common to the land-sea interface of tropical and subtropical intertidal zones. It has been reported that 60 to 75% of the coastline of the earth's tropical regions are lined with mangroves. Mangroves help prevent shoreline erosion, provide breeding, nesting and feeding areas for many marine animals and birds. Mangroves are important contributors of primary production in the coastal environment, and this is largely proportional to the standing crop of leaf chlorophylls. Higher intensities of ultraviolet radiation, resulting from stratospheric ozone depletion, can lead to a reduction of chlorophyll in terrestrial plants. Since the most common method for determining chlorophyll concentration is by extraction and this is labor intensive and time consuming, few studies on photosynthetic pigments of mangroves have been reported. Chlorophyll meter readings have been related to leaf chlorophyll content in apples and maples. It has also been correlated to nitrogen status in corn and cotton. Peterson et al., (1993) used a chlorophyll meter to detect nitrogen deficiency in crops and in determining the need for additional nitrogen fertilizer. Efforts to correlate chlorophyll meter measurements to chlorophyll content of mangroves have not been reported. This paper describes the use of a hand-held chlorophyll meter (Minolta SPAD-502) to determine the amount of red mangrove foliar chlorophyll present in the field.

Linking phytoplankton nitrogen uptake, macronutrients and chlorophyll- a in SW Atlantic waters: The case of the Gulf of San Jorge, Argentina

Science.gov (United States)

Paparazzo, Flavio E.; Williams, Gabriela N.; Pisoni, Juan P.; Solís, Miriam; Esteves, José L.; Varela, Diana E.

2017-08-01

We compared biological and chemical parameters in surface waters of the Gulf of San Jorge to better understand carbon export and the factors that control phytoplankton production in an area of the Argentinian Continental Shelf, a vastly under sampled region of the SW Atlantic Ocean. In April of 2012, we estimated new and regenerated primary production in the Gulf by measuring nitrate and ammonium uptake, respectively. We also measured macronutrient, and in situ chlorophyll-a concentrations, which were compared to chlorophyll-a estimates from remote sensing. Although the Gulf of San Jorge presents high levels of chlorophyll-a and primary production, the relationship between these parameters is not straightforward. Previous studies showed that surface chlorophyll-a explains only part of the variance in euphotic-zone integrated primary production, and that satellite-derived chlorophyll-a underestimates in situ primary production. Our results showed large spatial variability in the Gulf, with transitional physico-chemical conditions, such as fronts, that could favor an increase in biological production. In situ chlorophyll-a concentrations were highest at the mid-shelf station (6.0 mg m- 3) and lowest at the northernmost location by an order of magnitude. Remote sensing measurements of chlorophyll-a underestimated our in situ chlorophyll-a concentrations. Total nitrogen (nitrate + ammonium) uptake showed relatively similar rates throughout the study area (≈ 130 nM-N d- 1), except in the northernmost station where it was much lower (53 nM-N d- 1). This north region had a distinct water mass and maximal levels of macronutrients (nitrate ≈ 6 μM, ammonium ≈ 1.2 μM, phosphate ≈ 1.2 μM and silicic acid ≈ 4 μM). For the entire sampling region, chlorophyll-a concentrations strongly correlated with total nitrogen uptake (r = 0.76, n = 8, p < 0.05) and new primary production (r = 0.78, n = 8, p < 0.05). Values of the f-ratio were 0.9 in mid-shelf, and ranged

The biogeochemistry of nutrients, dissolved oxygen and chlorophyll a in the Catalan Sea (NW Mediterranean Sea

Directory of Open Access Journals (Sweden)

Mariona Segura-Noguera

2016-09-01

Full Text Available Reference depth profiles of dissolved inorganic nutrients, dissolved oxygen and chlorophyll a are described for the Catalan Sea using quality controlled data. Phosphate, nitrate and silicate show typical nutrient profiles, with nutriclines at different depths. Maximums of nitrite, dissolved oxygen and occasionally ammonium are found within the photic zone, close to the deep chlorophyll maximum. In intermediate waters we found a minimum of dissolved oxygen coincident with maximum concentrations of phosphate and nitrate. Ammonium concentration is unexpectedly high in the mesopelagic zone, where there are still measurable nitrite concentrations. The origin of such high ammonium and nitrite concentrations remains unclear. We also identify and describe anomalous data and profiles resulting from eutrophication, western Mediterranean Deep Water formation and dense shelf water cascading. The N:P ratio in deep waters is 22.4, which indicates P limitation relative to the Redfield ratio. However, the N:P ratio above the deep chlorophyll maximum in stratified surface waters is < 4 (< 8 including ammonium. The depth profiles of key biogeochemical variables described in this study will be a useful reference for future studies in the Catalan Sea (NW Mediterranean Sea in order to validate data sampled in this area, to identify anomalous processes, and to study the evolution of the ecosystem following the undergoing global change.

Variability in the correlation between Asian dust storms and chlorophyll a concentration from the North to Equatorial Pacific.

Directory of Open Access Journals (Sweden)

Sai-Chun Tan

Full Text Available A long-term record of Asian dust storms showed seven high-occurrence-frequency centers in China. The intrusion of Asian dust into the downwind seas, including the China seas, the Sea of Japan, the subarctic North Pacific, the North Pacific subtropical gyre, and the western and eastern Equatorial Pacific, has been shown to add nutrients to ocean ecosystems and enhance their biological activities. To explore the relationship between the transported dust from various sources to the six seas and oceanic biological activities with different nutrient conditions, the correlation between monthly chlorophyll a concentration in each sea and monthly dust storm occurrence frequencies reaching the sea during 1997-2007 was examined in this study. No correlations were observed between dust and chlorophyll a concentration in the 50 m China seas and the North Pacific subtropical gyre, the correlation coefficients were in the range 0.32-0.57. The correlation coefficients for the western and eastern Equatorial Pacific were relatively low (<0.36. These correlation coefficients were further interpreted in terms of the geographical distributions of dust sources, the transport pathways, the dust deposition, the nutrient conditions of oceans, and the probability of dust storms reaching the seas.

Recent Trends in Global Ocean Chlorophyll

Science.gov (United States)

Gregg, Watson; Casey, Nancy

2004-01-01

Recent analyses of SeaWiFS data have shown that global ocean chlorophyll has increased more than 5% since 1998. The North Pacific ocean basin has increased nearly 19%. To understand the causes of these trends we have applied the newly developed NASA Ocean Biogeochemical Assimilation Model (OBAM), which is driven in mechanistic fashion by surface winds, sea surface temperature, atmospheric iron deposition, sea ice, and surface irradiance. The mode1 utilizes chlorophyll from SeaWiFS in a daily assimilation. The model has in place many of the climatic variables that can be expected to produce the changes observed in SeaWiFS data. Ths enables us to diagnose the model performance, the assimilation performance, and possible causes for the increase in chlorophyll.

Impact of the river Liffey discharge on nutrient and chlorophyll concentrations in the Liffey estuary and Dublin Bay (Irish Sea)

Science.gov (United States)

O'Higgins, T. G.; Wilson, J. G.

2005-08-01

Temperature, salinity, nutrients (total oxidised nitrogen (TON), ammonium (NH 4) and orthophosphate (PO 4)) and chlorophyll a were monitored in the Liffey estuary and Dublin Bay from June 2000 to June 2003. Four groups of sites were defined comprising the upper estuary (Gp. I), the outer estuary (Gp. III) with a small set (Gp. II) of sites between Groups I and III heavily influenced by the sewage treatment works outflow, and the Bay proper (Gp. IV). Riverine inputs of TON and PO 4 were calculated at an average of 826 t N y -1 and 31 t P y -1, respectively, and were largely controlled by flow rate. The sewage treatment works were identified as a major source of PO 4 and NH 4 to the system. Mixing in the upper estuary of nutrient limited saline waters with hypernutrified river water regularly (i.e. annually) produced relatively high concentrations of chlorophyll a (>10 mg chl a m -3), and also sporadic blooms with extremely high chlorophyll a values (max. 121.6 mg chl a m -3). These latter phytoplankton blooms occurred in high salinity waters and were due to mixing of nutrient limited saline waters and nutrient rich river waters. The mean annual flux of phytoplankton carbon from the river Liffey was calculated at 23.5 t C y -1, of which half was accumulated or remineralised in the estuary and did not enter the Bay. In the Bay proper (Gp. IV) summer nutrient concentrations dropped below detection limits, and chlorophyll a concentrations followed the classic pattern with a spring bloom maximum of 5.5 mg chl a m -3. This pattern in nutrients and chlorophyll a came from the advection of waters into the Bay from an offshore source. Overall while there was considerable evidence for eutrophication in the estuary, the bay itself showed little biological response to nutrient loading.

Identification of the 7-Hydroxymethyl Chlorophyll a Reductase of the Chlorophyll Cycle in Arabidopsis[W

Science.gov (United States)

Meguro, Miki; Ito, Hisashi; Takabayashi, Atsushi; Tanaka, Ryouichi; Tanaka, Ayumi

2011-01-01

The interconversion of chlorophyll a and chlorophyll b, referred to as the chlorophyll cycle, plays a crucial role in the processes of greening, acclimation to light intensity, and senescence. The chlorophyll cycle consists of three reactions: the conversions of chlorophyll a to chlorophyll b by chlorophyllide a oxygenase, chlorophyll b to 7-hydroxymethyl chlorophyll a by chlorophyll b reductase, and 7-hydroxymethyl chlorophyll a to chlorophyll a by 7-hydroxymethyl chlorophyll a reductase. We identified 7-hydroxymethyl chlorophyll a reductase, which is the last remaining unidentified enzyme of the chlorophyll cycle, from Arabidopsis thaliana by genetic and biochemical methods. Recombinant 7-hydroxymethyl chlorophyll a reductase converted 7-hydroxymethyl chlorophyll a to chlorophyll a using ferredoxin. Both sequence and biochemical analyses showed that 7-hydroxymethyl chlorophyll a reductase contains flavin adenine dinucleotide and an iron-sulfur center. In addition, a phylogenetic analysis elucidated the evolution of 7-hydroxymethyl chlorophyll a reductase from divinyl chlorophyllide vinyl reductase. A mutant lacking 7-hydroxymethyl chlorophyll a reductase was found to accumulate 7-hydroxymethyl chlorophyll a and pheophorbide a. Furthermore, this accumulation of pheophorbide a in the mutant was rescued by the inactivation of the chlorophyll b reductase gene. The downregulation of pheophorbide a oxygenase activity is discussed in relation to 7-hydroxymethyl chlorophyll a accumulation. PMID:21934147

An overview of remote sensing of chlorophyll fluorescence

Science.gov (United States)

Xing, Xiao-Gang; Zhao, Dong-Zhi; Liu, Yu-Guang; Yang, Jian-Hong; Xiu, Peng; Wang, Lin

2007-03-01

Besides empirical algorithms with the blue-green ratio, the algorithms based on fluorescence are also important and valid methods for retrieving chlorophyll-a concentration in the ocean waters, especially for Case II waters and the sea with algal blooming. This study reviews the history of initial cognitions, investigations and detailed approaches towards chlorophyll fluorescence, and then introduces the biological mechanism of fluorescence remote sensing and main spectral characteristics such as the positive correlation between fluorescence and chlorophyll concentration, the red shift phenomena. Meanwhile, there exist many influence factors that increase complexity of fluorescence remote sensing, such as fluorescence quantum yield, physiological status of various algae, substances with related optical property in the ocean, atmospheric absorption etc. Based on these cognitions, scientists have found two ways to calculate the amount of fluorescence detected by ocean color sensors: fluorescence line height and reflectance ratio. These two ways are currently the foundation for retrieval of chlorophyl l - a concentration in the ocean. As the in-situ measurements and synchronous satellite data are continuously being accumulated, the fluorescence remote sensing of chlorophyll-a concentration in Case II waters should be recognized more thoroughly and new algorithms could be expected.

Flood-Induced Surface Blooms Alter Deep Chlorophyll Maxima Community Structure in Lake Michigan.

Science.gov (United States)

Aguilar, C.; Cuhel, R. L.; Seline, L.

2008-12-01

Watershed-wide floods can bring increased nutrients and phytoplankton to receiving waters. This input can alter physical, chemical and phytoplankton community structure in a major way. Phytoplankton species composition and size distribution are key factors in their use as ecological indicators. Since 2003, phytoplankton communities in Lake Michigan have shifted from diatom and big cell (>10μm)- dominated to small cell picocyanobacteria-dominated phytoplankton (Quagga Mussels, dampened seasonal cycling of silicate indicated a basin-wide reduction of diatom production, and unicellular Cyanobacteria became dominant in deep chlorophyll maximum (DCM) zones. In the DCM, Synechococcus-like cells reached populations of at least 210,000 cells/ml. DCM chlorophyll (chl) remained similar (3-4μg/l) but late summer species composition changed dramatically to mostly 10μm fraction increased from previous years, and over 75% of the particulate Si was also in this size fraction. Because of the rapid sinking of diatoms during calm weather of late June-early July of 2008, particulate Si did not reach high values in surfaces waters (ca. 1.5μM) but remained at a consistently higher level than in 2007. Sinking of diatoms from the surface depleted chl in a progression from inshore to offshore during July 2008. In July surface chl was higher 40-70 km offshore than in the coastal zone. Surface phytoplankton waxed and waned in population density as if a wave or lens moved continuously further offshore, with sinking cells depleting the surface algae following behind the crest. In the wake, strong DCM populations with higher chl and particulate Si accumulated in the 30-45m zone at the bottom of the thermocline. However, in 2008 DCM zones, picocyanobacteria attained only 70,000 cells/mL, one-third of the same dates in 2007. The ratio of chlorophyll per Synechococcus cell in 2008 was about 5-fold higher than in 2007, corroborating microscopic observations of lowered picoplanktonic abundance

Evaluation of Chlorophyll Content and Chlorophyll Fluorescence Parameters and Relationships between Chlorophyll a, b and Chlorophyll Content Index under Water Stress in Olea europaea cv. Dezful

OpenAIRE

E. Khaleghi; K. Arzani; N. Moallemi; M. Barzegar

2012-01-01

This study was conducted to determine effect of water stress on chlorophyll content and chlorophyll fluorescence parameter in young `Dezful- olive trees. Three irrigation regimes (40% ETcrop, 65% ETcrop and 100% ETcrop) were used. After irrigation treatments were applied, some of biochemical parameters including chlorophyll a, b, total chlorophyll, chlorophyll fluorescence and also chlorophyll content index (C.C.I) were measured. Results of Analysis of variance showed that irrigation treatmen...

An algorithm for detecting trophic status (chlorophyll-a), cyanobacterial-dominance, surface scums and floating vegetation in inland and coastal waters

CSIR Research Space (South Africa)

Matthews, MW

2012-09-01

Full Text Available A novel algorithm is presented for detecting trophic status (chlorophyll-a), cyanobacterial blooms (cyano-blooms), surface scum and floating vegetation in coastal and inland waters using top-ofatmosphere data from the Medium Resolution Imaging...

Seasonal and nonseasonal variability of satellite-derived chlorophyll and colored dissolved organic matter concentration in the California Current

Science.gov (United States)

Kahru, Mati; Mitchell, B. Greg

2001-02-01

Time series of surface chlorophyll a concentration (Chl) and colored dissolved organic matter (CDOM) derived from the Ocean Color and Temperature Sensor and Sea-Viewing Wide Field-of-View Sensor were evaluated for the California Current area using regional algorithms. Satellite data composited for 8-day periods provide the ability to describe large-scale changes in surface parameters. These changes are difficult to detect based on in situ observations alone that suffer from undersampling the large temporal and spatial variability, especially in Chl. We detected no significant bias in satellite Chl estimates compared with ship-based measurements. The variability in CDOM concentration was significantly smaller than that in Chl, both spatially and temporally. While being subject to large interannual and short-term variations, offshore waters (100-1000 km from the shore) have an annual cycle of Chl and CDOM with a maximum in winter-spring (December-March) and a minimum in late summer. For inshore waters the maximum is more likely in spring (April-May). We detect significant increase in both Chl and CDOM off central and southern California during the La Niña year of 1999. The trend of increasing Chl and CDOM from October 1996 to June 2000 is statistically significant in many areas.

Assessing the Variability in the Relationship Between the Particulate Backscattering Coefficient and the Chlorophyll a Concentration From a Global Biogeochemical-Argo Database

Science.gov (United States)

Barbieux, Marie; Uitz, Julia; Bricaud, Annick; Organelli, Emanuele; Poteau, Antoine; Schmechtig, Catherine; Gentili, Bernard; Obolensky, Grigor; Leymarie, Edouard; Penkerc'h, Christophe; D'Ortenzio, Fabrizio; Claustre, Hervé

2018-02-01

Characterizing phytoplankton distribution and dynamics in the world's open oceans requires in situ observations over a broad range of space and time scales. In addition to temperature/salinity measurements, Biogeochemical-Argo (BGC-Argo) profiling floats are capable of autonomously observing at high-frequency bio-optical properties such as the chlorophyll fluorescence, a proxy of the chlorophyll a concentration (Chla), the particulate backscattering coefficient (bbp), a proxy of the stock of particulate organic carbon, and the light available for photosynthesis. We analyzed an unprecedented BGC-Argo database of more than 8,500 multivariable profiles collected in various oceanic conditions, from subpolar waters to subtropical gyres. Our objective is to refine previously established Chla versus bbp relationships and gain insights into the sources of vertical, seasonal, and regional variability in this relationship. Despite some regional, seasonal and vertical variations, a general covariation occurs at a global scale. We distinguish two main contrasted situations: (1) concomitant changes in Chla and bbp that correspond to actual variations in phytoplankton biomass, e.g., in subpolar regimes; (2) a decoupling between the two variables attributed to photoacclimation or changes in the relative abundance of nonalgal particles, e.g., in subtropical regimes. The variability in the bbp:Chla ratio in the surface layer appears to be essentially influenced by the type of particles and by photoacclimation processes. The large BGC-Argo database helps identifying the spatial and temporal scales at which this ratio is predominantly driven by one or the other of these two factors.

Indian Ocean Dipole and El Niño/Southern Oscillation impacts on regional chlorophyll anomalies in the Indian Ocean

Directory of Open Access Journals (Sweden)

J. C. Currie

2013-10-01

Full Text Available The Indian Ocean Dipole (IOD and the El Niño/Southern Oscillation (ENSO are independent climate modes, which frequently co-occur, driving significant interannual changes within the Indian Ocean. We use a four-decade hindcast from a coupled biophysical ocean general circulation model, to disentangle patterns of chlorophyll anomalies driven by these two climate modes. Comparisons with remotely sensed records show that the simulation competently reproduces the chlorophyll seasonal cycle, as well as open-ocean anomalies during the 1997/1998 ENSO and IOD event. Results suggest that anomalous surface and euphotic-layer chlorophyll blooms in the eastern equatorial Indian Ocean in fall, and southern Bay of Bengal in winter, are primarily related to IOD forcing. A negative influence of IOD on chlorophyll concentrations is shown in a region around the southern tip of India in fall. IOD also depresses depth-integrated chlorophyll in the 5–10° S thermocline ridge region, yet the signal is negligible in surface chlorophyll. The only investigated region where ENSO has a greater influence on chlorophyll than does IOD, is in the Somalia upwelling region, where it causes a decrease in fall and winter chlorophyll by reducing local upwelling winds. Yet unlike most other regions examined, the combined explanatory power of IOD and ENSO in predicting depth-integrated chlorophyll anomalies is relatively low in this region, suggestive that other drivers are important there. We show that the chlorophyll impact of climate indices is frequently asymmetric, with a general tendency for larger positive than negative chlorophyll anomalies. Our results suggest that ENSO and IOD cause significant and predictable regional re-organisation of chlorophyll via their influence on near-surface oceanography. Resolving the details of these effects should improve our understanding, and eventually gain predictability, of interannual changes in Indian Ocean productivity, fisheries

A bio-optical algorithm for the remote estimation of the chlorophyll-a concentration in case 2 waters

Energy Technology Data Exchange (ETDEWEB)

Gitelson, Anatoly A; Gurlin, Daniela; Moses, Wesley J; Barrow, Tadd, E-mail: agitelson2@unl.ed [Center for Advanced Land Management Information Technologies (CALMIT), School of Natural Resources, University of Nebraska-Lincoln (United States)

2009-10-15

The objective of this work was to test the performance of a recently developed three-band model and its special case, a two-band model, for the remote estimation of the chlorophyll- a (chl-a) concentration in turbid productive case 2 waters. We specifically focused on (a) determining the ability of the models to estimate chl- a <20 mg m{sup -3}, typical for coastal and estuarine waters, and (b) assessing the potential of MODIS and MERIS to estimate chl-a concentrations in turbid productive waters, using red and near-infrared (NIR) bands. Reflectance spectra and water samples were collected in 89 stations over lakes in the United States with a wide variability in optical parameters (i.e. 2.1a<184 mg m{sup -3}; 0.5a variation for chl- a ranging from 2 to 20 mg m{sup -3} and can be used to estimate chlorophyll-a concentrations with a root mean square error (RMSE) of <1.65 mg m{sup -3}. MODIS (bands 13 and 15) and MERIS (bands 7, 9, and 10) red and NIR reflectances were simulated from the collected reflectance spectra and potential estimation errors were assessed. The MODIS two-band model is able to estimate chl- a concentrations with a RMSE of<7.5 mg m{sup -3} for chl-a ranging from 2 to 50 mg m{sup -3}; however, the model loses its sensitivity for chl- a<20 mg m{sup -3}. Benefiting from the higher spectral resolution of the MERIS data, the MERIS three-band model accounts for 93% of chl- a variation and is able to estimate chl-a concentrations with a RMSE of<5.1 mg m{sup -3} for chl-a ranging from 2 to 50 mg m{sup -3}, and a RMSE of<1.7 mg m{sup -3} for chl-a ranging from 2 to 20 mg m{sup -3}. These findings imply that, provided that an atmospheric correction scheme specific to the red and NIR spectral region is available, the extensive database of MODIS and MERIS images could be used to

Vertical Distributions of Coccolithophores, PIC, POC, Biogenic Silica, and Chlorophyll a Throughout the Global Ocean.

Science.gov (United States)

Balch, William M; Bowler, Bruce C; Drapeau, David T; Lubelczyk, Laura C; Lyczkowski, Emily

2018-01-01

Coccolithophores are a critical component of global biogeochemistry, export fluxes, and seawater optical properties. We derive globally significant relationships to estimate integrated coccolithophore and coccolith concentrations as well as integrated concentrations of particulate inorganic carbon (PIC) from their respective surface concentration. We also examine surface versus integral relationships for other biogeochemical variables contributed by all phytoplankton (e.g., chlorophyll a and particulate organic carbon) or diatoms (biogenic silica). Integrals are calculated using both 100 m integrals and euphotic zone integrals (depth of 1% surface photosynthetically available radiation). Surface concentrations are parameterized in either volumetric units (e.g., m -3 ) or values integrated over the top optical depth. Various relationships between surface concentrations and integrated values demonstrate that when surface concentrations are above a specific threshold, the vertical distribution of the property is biased to the surface layer, and when surface concentrations are below a specific threshold, the vertical distributions of the properties are biased to subsurface maxima. Results also show a highly predictable decrease in explained-variance as vertical distributions become more vertically heterogeneous. These relationships have fundamental utility for extrapolating surface ocean color remote sensing measurements to 100 m depth or to the base of the euphotic zone, well beyond the depths of detection for passive ocean color remote sensors. Greatest integrated concentrations of PIC, coccoliths, and coccolithophores are found when there is moderate stratification at the base of the euphotic zone.

Five Year Mean Surface Chlorophyll Estimates in the Northern Gulf of Mexico for 2005 through 2009

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — These images were created by combining the mean surface chlorophyll estimates to produce seasonal representations for winter, spring, summer and fall. Winter...

Control of quantum interference of an excitonic wave in a chlorophyll chain with a chlorophyll ring

International Nuclear Information System (INIS)

Hong, Suc-Kyoung; Nam, Seog-Woo; Yeon, Kyu-Hwang

2010-01-01

The quantum interference of an excitonic wave and its coherent control in a nanochain with a nanoring are studied. The nanochain is comprised of six chlorophylls, where four chlorophylls compose the nanoring and two chlorophylls are attached at two opposite sites on the nanoring. The exciton dynamics and the correlation of the excitation between chlorophylls are analyzed for a given configurational arrangement and dipolar orientation of the chlorophylls. The results of this study show that the excitation at specified chlorophylls is suppressed or enhanced by destructive or constructive interference of the excitonic wave in the chlorophyll nanochain.

Extracted chlorophyll-a and phaeopigment concentrations and other data from R/V Endeavor from 1995-01-11 to 1995-06-16 (NODC Accession 9900026)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains "extracted chlorophyll-a and phaeopigment" concentrations collected for the GLOBEC Georges Bank project by Dr. Dian Gifford, Univ. of Rhode...

Wind driven nutrient and subsurface chlorophyll-a enhancement in the Bay of La Paz, Gulf of California

Science.gov (United States)

Coria-Monter, Erik; Monreal-Gómez, María Adela; Salas de León, David Alberto; Durán-Campos, Elizabeth; Merino-Ibarra, Martín

2017-09-01

Nutrient and chlorophyll-a distributions in the Bay of La Paz, Gulf of California, Mexico were analyzed during the late spring of 2004 to assess their relations to hydrography and circulation patterns. The results show the presence of both Gulf of California Water and Subtropical Subsurface Water. Water circulation was dominated by wind stress driven cyclonic circulation along f / H contours (f is planetary vorticity and H is depth), and upwelling resulting from the divergence shows a vertical velocity of ∼0.4 m d-1. Nutrient concentrations were higher in the center of the cyclonic pattern, where a rise in the nutricline contributed nutrients to the euphotic layer as a result of Ekman pumping. The vertical section showed the presence of a chlorophyll-a maximum at the thermocline shoaling to a depth of only 12 m. Along the surface, two peaks of chlorophyll-a were observed, one at Boca Grande and another off San Juan de la Costa, associated with upwelling and mixing derived from current interactions with abrupt topographies. The chlorophyll-a maximum increased from 0.8 mg m-3 in the external part of the cyclonic pattern to 2.0 mg m-3 in its center. The vertically integrated chlorophyll-a concentrations followed a similar pattern, rising from 10 to 20 mg m-2 and reaching their highest values in the center of the cyclonic circulation pattern. A schematic model was developed to describe processes that occur in late spring: the wind stress driven cyclonic structure promotes upward nutrient flux, which in turn drives an enhancement of chlorophyll-a. Upwelling was found to be the main mechanism of fertilization responsible for the enhancement of productivity levels by means of nutrient transport into the euphotic zone during spring. Other chlorophyll enhancement areas point to the occurrence of additional fertilization processes that may derive from interactions between cyclonic circulation patterns and the topography off of San Juan de la Costa, where phosphate mining

Distribution of nutrients, chlorophyll and phytoplankton primary ...

African Journals Online (AJOL)

Distribution of nutrients, chlorophyll and phytoplankton primary production in ... Two cruises were undertaken in the vicinity of the Cape Frio upwelling cell ... and concentrations of nitrate, phosphate, silicate, oxygen and chlorophyll a. ... Estimates of the annual primary production for each of the water bodies were calculated.

Chlorophyll-a, Aqua MODIS, NPP, 0.0125 degrees, Indonesia

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from NASA's Aqua Spacecraft. Measurements are gathered by the Moderate Resolution Imaging...

Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis.

Science.gov (United States)

Lin, Yao-Pin; Wu, Meng-Chen; Charng, Yee-Yung

2016-12-01

Chlorophyll turns over in green organs during photosystem repair and is salvaged via de- and rephytylation, but the enzyme involved in dephytylation is unknown. We have identified an Arabidopsis thaliana thylakoid protein with a putative hydrolase domain that can dephytylate chlorophyll in vitro and in vivo. The corresponding locus, CHLOROPHYLL DEPHYTYLASE1 (CLD1), was identified by mapping a semidominant, heat-sensitive, missense allele (cld1-1). CLD1 is conserved in oxygenic photosynthetic organisms, sharing structural similarity with pheophytinase, which functions in chlorophyll breakdown during leaf senescence. Unlike pheophytinase, CLD1 is predominantly expressed in green organs and can dephytylate chlorophyll in vitro. The specific activity is significantly higher for the mutant protein encoded by cld1-1 than the wild-type enzyme, consistent with the semidominant nature of the cld1-1 mutation. Supraoptimal CLD1 activities in cld1-1 mutants and transgenic seedlings led to the proportional accumulation of chlorophyllides derived from chlorophyll dephytylation after heat shock, which resulted in light-dependent cotyledon bleaching. Reducing CLD1 expression diminished thermotolerance and the photochemical efficiency of photosystem II under prolonged moderate heat stress. Taken together, our results suggest that CLD1 is the long-sought enzyme for removing the phytol chain from chlorophyll during its turnover at steady state within the chloroplast. © 2016 American Society of Plant Biologists. All rights reserved.

Relationship between chlorophyll-a and column primary production

Digital Repository Service at National Institute of Oceanography (India)

Dalal, S.G.; Bhargava, R.M.S.

Relationship between surface chlorophyll a and column primary production has been established to help in estimating the latter more quickly and accurately. The equation derived is Primary Production, y = 0.54 Ln Chl a - 0.6. The relationship...

Assessment of a global climatology of oceanic dimethylsulfide (DMS) concentrations based on SeaWiFS imagery (1998-2001)

NARCIS (Netherlands)

Belviso, S; Moulin, C; Bopp, L; Stefels, J

A method is developed to estimate sea-surface particulate dimethylsulfoniopropionate (DMSP(p)) and dimethylsulfide (DMS) concentrations from sea-surface concentrations of chlorophyll a (Chl a). When compared with previous studies, the 1degrees x 1degrees global climatology of oceanic DMS

Time-resolved interaction investigations of carbocyanine dyes and chlorophyll a in solution

International Nuclear Information System (INIS)

Feller, K.H.; Fassler, D.

1983-01-01

Using a Nd:YAG laser/streak camera system of 30 ps time resolution the quenching of the fluorescence of the carbocyanine dye ICC by chlorophyll a in methanolic solution was investigated. The fluorescence lifetime of ICC decreased within the chlorophyll concentration range 0 - 9x10 - 5 mol/l from 170 ps to 135 ps. The observed very effective fluorescence quenching process suggests that the formation of heteroaggregates from ICC and chlorophyll is responsible for the rapid energy transfer. (author)

Regional ocean-colour chlorophyll algorithms for the Red Sea

KAUST Repository

Brewin, Robert J.W.

2015-05-18

The Red Sea is a semi-enclosed tropical marine ecosystem that stretches from the Gulf of Suez and Gulf of Aqaba in the north, to the Gulf of Aden in the south. Despite its ecological and economic importance, its biological environment is relatively unexplored. Satellite ocean-colour estimates of chlorophyll concentration (an index of phytoplankton biomass) offer an observational platform to monitor the health of the Red Sea. However, little is known about the optical properties of the region. In this paper, we investigate the optical properties of the Red Sea in the context of satellite ocean-colour estimates of chlorophyll concentration. Making use of a new merged ocean-colour product, from the European Space Agency (ESA) Climate Change Initiative, and in situ data in the region, we test the performance of a series of ocean-colour chlorophyll algorithms. We find that standard algorithms systematically overestimate chlorophyll when compared with the in situ data. To investigate this bias we develop an ocean-colour model for the Red Sea, parameterised to data collected during the Tara Oceans expedition, that estimates remote-sensing reflectance as a function of chlorophyll concentration. We used the Red Sea model to tune the standard chlorophyll algorithms and the overestimation in chlorophyll originally observed was corrected. Results suggest that the overestimation was likely due to an excess of CDOM absorption per unit chlorophyll in the Red Sea when compared with average global conditions. However, we recognise that additional information is required to test the influence of other potential sources of the overestimation, such as aeolian dust, and we discuss uncertainties in the datasets used. We present a series of regional chlorophyll algorithms for the Red Sea, designed for a suite of ocean-colour sensors, that may be used for further testing.

Carbon-14 labeling of phytoplankton carbon and chlorophyll a carbon: determination of specific growth rates

International Nuclear Information System (INIS)

Welschmeyer, N.A.; Lorenzen, C.J.

1984-01-01

The pattern of photosynthetic 14 C labeling over time is described for phytoplankton. The carbon-specific growth rate (d -1 ) is defined explicitly by changes in the specific activity (dpm μg -1 C) of the algae. For Skeletonema costatum, growing in axenic batch culture, the specific activities of both total cellular carbon and chlorophyll carbon increase at equal rates and the change in specific activity with time follows the predicted pattern. The specific activity of 14 C-labeled chlorophyll a was used to estimate phytoplankton growth rates and C:Chl ratios of field samples in Dabob Bay (Puget Sound), Washington. Growth rates decreased with depth and C:Chl ratios were higher for samples incubated under high light intensity. In several instances the C:Chl ratio increased from the beginning to the end of the incubation; this trend was most conspicuous near surface light intensities and for days of high total incident radiation. On these occasions, Chl a was actively 14 C labeled, yet little (or even negative) change was noted in the concentration of Chl a. These results suggest that some process (or processes) of chlorophyll degradation must be active at the same time that chlorophyll is being synthesized

A Statistical Algorithm for Estimating Chlorophyll Concentration in the New Caledonian Lagoon

Directory of Open Access Journals (Sweden)

Guillaume Wattelez

2016-01-01

Full Text Available Spatial and temporal dynamics of phytoplankton biomass and water turbidity can provide crucial information about the function, health and vulnerability of lagoon ecosystems (coral reefs, sea grasses, etc.. A statistical algorithm is proposed to estimate chlorophyll-a concentration ([chl-a] in optically complex waters of the New Caledonian lagoon from MODIS-derived “remote-sensing” reflectance (Rrs. The algorithm is developed via supervised learning on match-ups gathered from 2002 to 2010. The best performance is obtained by combining two models, selected according to the ratio of Rrs in spectral bands centered on 488 and 555 nm: a log-linear model for low [chl-a] (AFLC and a support vector machine (SVM model or a classic model (OC3 for high [chl-a]. The log-linear model is developed based on SVM regression analysis. This approach outperforms the classical OC3 approach, especially in shallow waters, with a root mean squared error 30% lower. The proposed algorithm enables more accurate assessments of [chl-a] and its variability in this typical oligo- to meso-trophic tropical lagoon, from shallow coastal waters and nearby reefs to deeper waters and in the open ocean.

One-electron oxidation of photosynthetic pigments in micelles. Bacteriochlorophyll a, chlorophyll a, chlorophyll b, and pheophytin a

International Nuclear Information System (INIS)

Chauvet, J.P.

1981-01-01

Chlorophyll a, chlorophyll b, and bacteriochlorophyll a in aqueous micellar solutions of Trition X 100 (2%) are readily oxidized by pulse-radiolytically generated N 3 ., Br 2 - ., and (SCN) 2 - . radicals at nearly diffusion-controlled rates. The kinetic study suggests that pigment molecules occupy multiple sites in the micelle. Pheophytin a is only oxidized by N 3 . and Br 2 - . radicals. The absolute spectra and the molar extinction coefficients of chlorophyll a, bacteriochlorophyll a, chlorophyll b, and pheophytin a cations have been determined. The chlorophyll a cation has been observed in the presence of pigment aggregates

Efficiency of portable chlorophyll meters in assessing the nutritional status of wheat plants

Directory of Open Access Journals (Sweden)

Alessana F. Schlichting

2015-12-01

Full Text Available ABSTRACT The objective of this study was to verify the efficiency of two portable chlorophyll meters (Minolta SPAD® 502 and Falker ClorofiLOG® 1030 in assessing the nutritional status of wheat plants, correlating the indices from the devices and the direct determination of chlorophyll content with the concentration of nitrogen (N in the plant. The experiment was conducted in a greenhouse, in pots with 5 dm3 of Oxisol, in a completely randomized design, with six N doses (0, 80, 160, 240, 320 and 400 mg dm-3 and five replicates. At 47 days after emergence, the readings of SPAD and Falker indices and the quantification of chlorophyll content and N concentration in wheat plants were performed, as well as analysis of variance and correlation test, both at 0.05 probability level. The chlorophyll meters Minolta SPAD® 502 and Falker ClorofiLOG® 1030 do not differ with respect to the indirect determination of chlorophyll in wheat plants. The Falker chlorophyll index was statistically equal to the chlorophyll content. Indirect chlorophyll indices and chlorophyll content showed a high correlation with the N concentration in the plant.

Influence of Typhoon Matsa on Phytoplankton Chlorophyll-a off East China.

Science.gov (United States)

Zhao, Hui; Shao, Jinchao; Han, Guoqi; Yang, Dezhou; Lv, Jianhai

2015-01-01

Typhoons can cause strong disturbance, mixing, and upwelling in the upper layer of the oceans. Rich nutrients from the subsurface layer can be brought to the euphotic layer, which will induce the phytoplankton to breed and grow rapidly. In this paper, we investigate the impact of an intense and fast moving tropical storm, Typhoon Matsa, on phytoplankton chlorophyll-a (Chl-a) concentration off East China. By using satellite remote sensing data, we analyze the changes of Chl-a concentration, Sea Surface Temperature (SST) and wind speed in the pre- and post-typhoon periods. We also give a preliminary discussion on the different responses of the Chl-a concentration between nearshore and offshore waters. In nearshore/coastal regions where nutrients are generally rich, the Chl-a maximum occurs usually at the surface or at the layer close to the surface. And, in offshore tropical oligotrophic oceans, the subsurface maxima of Chl-a exist usually in the stratified water column. In an offshore area east of Taiwan, the Chl-a concentration rose gradually in about two weeks after the typhoon. However, in a coastal area north of Taiwan high Chl-a concentration decreased sharply before landfall, rebounded quickly to some degree after landfall, and restored gradually to the pre-typhoon level in about two weeks. The Chl-a concentration presented a negative correlation with the wind speed in the nearshore area during the typhoon, which is opposite to the response in the offshore waters. The phenomena may be attributable to onshore advection of low Chl-a water, coastal downwelling and intensified mixing, which together bring pre-typhoon surface Chl-a downward in the coastal area. In the offshore area, the typhoon may trigger increase of Chl-a concentration through uptake of nutrients by typhoon-induced upwelling and entrainment mixing.

Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll-Protein Complex.

Science.gov (United States)

Hitchcock, Andrew; Jackson, Philip J; Chidgey, Jack W; Dickman, Mark J; Hunter, C Neil; Canniffe, Daniel P

2016-09-16

Improvements to photosynthetic efficiency could be achieved by manipulating pigment biosynthetic pathways of photosynthetic organisms in order to increase the spectral coverage for light absorption. The development of organisms that can produce both bacteriochlorophylls and chlorophylls is one way to achieve this aim, and accordingly we have engineered the bacteriochlorophyll-utilizing anoxygenic phototroph Rhodobacter sphaeroides to make chlorophyll a. Bacteriochlorophyll and chlorophyll share a common biosynthetic pathway up to the precursor chlorophyllide. Deletion of genes responsible for the bacteriochlorophyll-specific modifications of chlorophyllide and replacement of the native bacteriochlorophyll synthase with a cyanobacterial chlorophyll synthase resulted in the production of chlorophyll a. This pigment could be assembled in vivo into the plant water-soluble chlorophyll protein, heterologously produced in Rhodobacter sphaeroides, which represents a proof-of-principle for the engineering of novel antenna complexes that enhance the spectral range of photosynthesis.

Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis[OPEN

Science.gov (United States)

2016-01-01

Chlorophyll turns over in green organs during photosystem repair and is salvaged via de- and rephytylation, but the enzyme involved in dephytylation is unknown. We have identified an Arabidopsis thaliana thylakoid protein with a putative hydrolase domain that can dephytylate chlorophyll in vitro and in vivo. The corresponding locus, CHLOROPHYLL DEPHYTYLASE1 (CLD1), was identified by mapping a semidominant, heat-sensitive, missense allele (cld1-1). CLD1 is conserved in oxygenic photosynthetic organisms, sharing structural similarity with pheophytinase, which functions in chlorophyll breakdown during leaf senescence. Unlike pheophytinase, CLD1 is predominantly expressed in green organs and can dephytylate chlorophyll in vitro. The specific activity is significantly higher for the mutant protein encoded by cld1-1 than the wild-type enzyme, consistent with the semidominant nature of the cld1-1 mutation. Supraoptimal CLD1 activities in cld1-1 mutants and transgenic seedlings led to the proportional accumulation of chlorophyllides derived from chlorophyll dephytylation after heat shock, which resulted in light-dependent cotyledon bleaching. Reducing CLD1 expression diminished thermotolerance and the photochemical efficiency of photosystem II under prolonged moderate heat stress. Taken together, our results suggest that CLD1 is the long-sought enzyme for removing the phytol chain from chlorophyll during its turnover at steady state within the chloroplast. PMID:27920339

Determination of phytoplankton abundances (Chlorophyll-a) in the optically complex inland water - The Baltic Sea.

Science.gov (United States)

Zhang, Daoxi; Lavender, Samantha; Muller, Jan-Peter; Walton, David; Karlson, Bengt; Kronsell, Johan

2017-12-01

A novel approach, termed Summed Positive Peaks (SPP), is proposed for determining phytoplankton abundances (Chlorophyll-a or Chl-a) and surface phytoplankton bloom extent in the optically complex Baltic Sea. The SPP approach is established on the basis of a baseline subtraction method using Rayleigh corrected top-of-atmosphere data from the Medium Resolution Imaging Spectrometer (MERIS) measurements. It calculates the reflectance differences between phytoplankton related signals observed in the MERIS red and near infrared (NIR) bands, such as sun-induced chlorophyll fluorescence (SICF) and the backscattering at 709nm, and considers the summation of the positive line heights for estimating Chl-a concentrations. The SPP algorithm is calibrated against near coincident in situ data collected from three types of phytoplankton dominant waters encountered in the Baltic Sea during 2010 (N=379). The validation results show that the algorithm is capable of retrieving Chl-a concentrations ranging from 0.5 to 3mgm -3 , with an RMSE of 0.24mgm -3 (R 2 =0.69, N=264). Additionally, the comparison results with several Chl-a algorithms demonstrates the robustness of the SPP approach and its sensitivity to low to medium biomass waters. Based on the red and NIR reflectance features, a flagging method is also proposed to distinguish intensive surface phytoplankton blooms from the background water. Copyright © 2017 Elsevier B.V. All rights reserved.

A new method to generate a high-resolution global distribution map of lake chlorophyll

Science.gov (United States)

Sayers, Michael J; Grimm, Amanda G.; Shuchman, Robert A.; Deines, Andrew M.; Bunnell, David B.; Raymer, Zachary B; Rogers, Mark W.; Woelmer, Whitney; Bennion, David; Brooks, Colin N.; Whitley, Matthew A.; Warner, David M.; Mychek-Londer, Justin G.

2015-01-01

A new method was developed, evaluated, and applied to generate a global dataset of growing-season chlorophyll-a (chl) concentrations in 2011 for freshwater lakes. Chl observations from freshwater lakes are valuable for estimating lake productivity as well as assessing the role that these lakes play in carbon budgets. The standard 4 km NASA OceanColor L3 chlorophyll concentration products generated from MODIS and MERIS sensor data are not sufficiently representative of global chl values because these can only resolve larger lakes, which generally have lower chl concentrations than lakes of smaller surface area. Our new methodology utilizes the 300 m-resolution MERIS full-resolution full-swath (FRS) global dataset as input and does not rely on the land mask used to generate standard NASA products, which masks many lakes that are otherwise resolvable in MERIS imagery. The new method produced chl concentration values for 78,938 and 1,074 lakes in the northern and southern hemispheres, respectively. The mean chl for lakes visible in the MERIS composite was 19.2 ± 19.2, the median was 13.3, and the interquartile range was 3.90–28.6 mg m−3. The accuracy of the MERIS-derived values was assessed by comparison with temporally near-coincident and globally distributed in situmeasurements from the literature (n = 185, RMSE = 9.39, R2 = 0.72). This represents the first global-scale dataset of satellite-derived chl estimates for medium to large lakes.

Spatial and temporal variability of chlorophyll and primary productivity in surface waters of southern Chile (41.5 43° S)

Science.gov (United States)

Iriarte, J. L.; González, H. E.; Liu, K. K.; Rivas, C.; Valenzuela, C.

2007-09-01

The southern fjord region of Chile is a unique ecosystem characterized by complex marine-terrestrial-atmospheric interactions that result in high biological production. Since organic nitrogen from terrestrial and atmospheric compartments is highly significant in this region (>40%), as is the low NO 3:PO 4 ratio in surface waters, it is suggested that fertilization from inorganic and organic nitrogen sources has a strong influence on both phytoplankton biomass/primary production and harmful algae bloom dynamics. The data presented in this paper provide an opportunity to improve our knowledge of phytoplankton dynamics on temporal and spatial mesoscales. Ocean color data from NASA (SeaWiFS) for chlorophyll and primary production estimates and in situ surface measurement of inorganic nutrients, phytoplankton biomass, and primary productivity revealed that the coastal waters of southern Chile have a classical spring and autumn chlorophyll bloom cycle in which primary production is co-limited by strong seasonal changes in light and nitrate. During spring blooms, autotrophic biomass (such as chlorophyll a, Chl- a) and primary production estimates reached 25 mg Chl- a m -3 and 23 mg C m -3 h -1, respectively, and micro-phytoplankton accounted for a significant portion of the biomass (60%) in spring. The contribution of phytoplankton size classes to total chlorophyll a revealed the dominance of nanoplankton (>50%) in winter and post-bloom periods (<1.0 mg Chl- a m -3).

Regional ocean-colour chlorophyll algorithms for the Red Sea

KAUST Repository

Brewin, Robert J.W.; Raitsos, Dionysios E.; Dall'Olmo, Giorgio; Zarokanellos, Nikolaos; Jackson, Thomas; Racault, Marie-Fanny; Boss, Emmanuel S.; Sathyendranath, Shubha; Jones, Burton; Hoteit, Ibrahim

2015-01-01

an ocean-colour model for the Red Sea, parameterised to data collected during the Tara Oceans expedition, that estimates remote-sensing reflectance as a function of chlorophyll concentration. We used the Red Sea model to tune the standard chlorophyll

Microdroplet fusion mass spectrometry: accelerated kinetics of acid-induced chlorophyll demetallation.

Science.gov (United States)

Lee, Jae Kyoo; Nam, Hong Gil; Zare, Richard N

2017-01-01

Kinetics of acid-induced chlorophyll demetallation was recorded in microdroplets by fusing a stream of microdroplets containing 40 µM chlorophyll a or b dissolved in methanol with a stream of aqueous microdroplets containing 35 mM hydrochloric acid (pH = 1·46). The kinetics of the demetallation of chlorophyll in the fused microdroplets (14 ± 6 µm diameter; 84 ± 18 m s-1 velocity) was recorded by controlling the traveling distance of the fused microdroplets between the fusion region and the inlet of a mass spectrometer. The rate of acid-induced chlorophyll demetallation was about 960 ± 120 times faster in the charged microdroplets compared with that reported in bulk solution. If no voltage was applied to the sprayed microdroplets, then the acceleration factor was about 580 ± 90, suggesting that the applied voltage is not a major factor determining the acceleration. Chlorophyll a was more rapidly demetallated than chlorophyll b by a factor of ~26 in bulk solution and ~5 in charged microdroplets. The demetallation kinetics was second order in the H+ concentration, but the acceleration factor of microdroplets compared with bulk solution appeared to be unchanged in going from pH = 1·3 to 7·0. The water:methanol ratio of the fused microdroplets was varied from 7:3 to 3:7 causing an increase in the reaction rate of chlorophyll a demetallation by 20%. This observation demonstrates that the solvent composition, which has different evaporation rates, does not significantly affect the acceleration. We believe that a major portion of the acceleration can be attributed to confinement effects involving surface reactions rather than either to evaporation of solvents or to the introduction of charges to the microdroplets.

Effect of PEG-6000 Imposed Water Deficit on Chlorophyll Metabolism in Maize Leaves

Directory of Open Access Journals (Sweden)

Rekha Gadre

2013-08-01

Full Text Available Drought stress is one of the major abiotic constraint limiting plant growth and productivity world wide. The current study was undertaken with the aim to investigate the effect of water deficit imposed by PEG-6000, on chlorophyll metabolism in maize leaves to work out the mechanistic details. Leaf segments prepared from primary leaves of etiolated maize seedlings were treated with varying concentrations of polyethylene glycol-6000 (PEG-6000; w/v- 5%, 10%, 20%, 30% in continuous light of intensity 40 Wm-2 at 26±2 °C for 24 h in light chamber. The results demonstrate a concentration dependent decline in chlorophyll content with increasing concentration of polyethylene glycol-6000 (PEG-6000. Reduction in chlorophyll ‘a’ level was to a greater extent than the chlorophyll ‘b’. The RNA content decreased in a concentration dependent manner with PEG, however, proline content increased significantly. Relative water content decreased significantly with the supply of 30% PEG only. A substantial decrease in chlorophyll synthesis due to significant reduction in ALA content and ALAD activity, with no change in chlorophyllase activity with the supply of PEG suggests that water deficit affects chlorophyll formation rather than its degradation.

EmpiricalValues_Chlorophyll_GrandComposite

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This personal geodatabase contains raster images of chlorophyll concentrations in the Gulf of Maine. These raster images are a composite of several years (1997-2005)...

A modern robust approach to remotely estimate chlorophyll in coastal and inland zones

Science.gov (United States)

Shanmugam, Palanisamy; He, Xianqiang; Singh, Rakesh Kumar; Varunan, Theenathayalan

2018-05-01

The chlorophyll concentration of a water body is an important proxy for representing the phytoplankton biomass. Its estimation from multi or hyper-spectral remote sensing data in natural waters is generally achieved by using (i) the waveband ratioing in two or more bands in the blue-green or (ii) by using a combination of the radiance peak position and magnitude in the red-near-infrared (NIR) spectrum. The blue-green ratio algorithms have been extensively used with satellite ocean color data to investigate chlorophyll distributions in open ocean and clear waters and the application of red-NIR algorithms is often restricted to turbid productive water bodies. These issues present the greatest obstacles to our ability to formulate a modern robust method suitable for quantitative assessments of the chlorophyll concentration in a diverse range of water types. The present study is focused to investigate the normalized water-leaving radiance spectra in the visible and NIR region and propose a robust algorithm (Generalized ABI, GABI algorithm) for chlorophyll concentration retrieval based on Algal Bloom index (ABI) which separates phytoplankton signals from other constituents in the water column. The GABI algorithm is validated using independent in-situ data from various regional to global waters and its performance is further evaluated by comparison with the blue-green waveband ratios and red-NIR algorithms. The results revealed that GABI yields significantly more accurate chlorophyll concentrations (with uncertainties less than 13.5%) and remains more stable in different waters types when compared with the blue-green waveband ratios and red-NIR algorithms. The performance of GABI is further demonstrated using HICO images from nearshore turbid productive waters and MERIS and MODIS-Aqua images from coastal and offshore waters of the Arabian Sea, Bay of Bengal and East China Sea.

Instability of chlorophyll in yellow lupin seedlings grown in soil contaminated with ciprofloxacin and tetracycline.

Science.gov (United States)

Rydzyński, Dariusz; Piotrowicz-Cieślak, Agnieszka I; Grajek, Hanna; Michalczyk, Dariusz J

2017-10-01

With increasing soil concentrations of ciprofloxacin and tetracycline a decrease of leaf chlorophyll content was observed. Tetracycline was more detrimental than ciprofloxacin. The chlorophyll content in plants growing for ten days on a tetracycline containing soil decreased by 68%. The decrease of chlorophyll concentration was even sharper in new leaves that formed after application of the antibiotic (up to 81% drop). The comparison of absorption spectra of commercial, reagent grade chlorophyll, alone and incubated with antibiotics, has shown that ciprofloxacin and tetracycline can react directly with chlorophyll and decrease its concentration by 47.7% and 48.5%, respectively. The changes in fluorescence spectra confirmed the formation of chlorophyll degradation product. The chlorophyll decay was a second order reaction and depended on antibiotic concentration and duration of exposure. Reaction rate constants differed with antibiotics and their soil concentrations. With increasing contents of antibiotics in soil the constant of chlorophyll degradation rate in lupin plants increased from k = 870 M -1 day -1 for 3 mg ciprofloxacin to k = 2490 M -1 day -1 for 90 mg ciprofloxacin, and in the case of tetracycline the reaction rate constant increased from k = 1330 M -1 day -1 to k = 2910 M -1 day -1 . The sensitivity of chlorophyll to ciprofloxacin and tetracycline was confirmed by determining EC and TU indices. Copyright © 2017 Elsevier Ltd. All rights reserved.

Temporal Relationships Between African Dust and Chlorophyll-a in the Eastern Caribbean Basin

Science.gov (United States)

Gomez-Andujar, N. X.; Mayol-Bracero, O. L.; Torres-Delgado, E.

2017-12-01

Seasonal African Dust (AD) transports soluble iron to oligotrophic Caribbean waters, and when bioavailable, it could increase marine primary productivity (PP). Recently, the region has experienced the proliferation of unusually high quantities of Sargassum, an iron-absorbing macroalgae inhabiting the air-sea interface, which possess ecological and economic challenges and whose driving factors are still uncertain. AD events reach Puerto Rico (PR) mostly during boreal summer months. This is also the season when chlorophyll-α (CHL) concentrations are highest, when the algae starts to bloom, and when sediment plumes from the Orinoco River (ORP) also reach nutrient discharge maxima.This study seeks to better understand the temporal relationships between increases in chlorophyll-α and the presence of african dust events in the region. Aerosol data collected at the Cabezas de San Juan Atmospheric Observatory was used to identify AD events between January 2005 and December 2015. Light scattering coefficients were measured with an integrating Nephelometer, while light absorption coefficients were obtained from either the Particle Soot/Absorption Photometer (PSAP) or the Continuous Light Absorption Photometer (CLAP). Spectral properties suggesting AD events were cross-referenced with surface dust concentration image models and source-attributed air masses corresponding to dusty periods using Hybrid Single-Particle Lagrangian Integrated Trajectories (HYSPLIT). For all years with spectral data, modeled monthly wet dust deposition was correlated (r=0.64) with mean CHL concentrations from NASA's Moderate Resolution Imaging Spectroradiometer (MODIS). Daily dust mass column densities from NASA's MERRA-2 model were also correlated (r2= 0.53) to sea surface iron concentrations from NASA's Ocean Biogeochemical Model. We present the 2010 case study, which coincides with the start of the Sargassum bloom and shows CHL peaks occurring a month before ORPs but during the AD season

Chlorophyll-a, Aqua MODIS, OSU DB, 0.0125 degrees, West US

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from NASA's Aqua satellite. Measurements are gathered by the Moderate Resolution Imaging...

Chlorophyll-a, Aqua MODIS, NPP, 0.025 degrees, Pacific Ocean, EXPERIMENTAL

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from NASA's Aqua Spacecraft. Measurements are gathered by the Moderate Resolution Imaging...

Chlorophyll-a, Aqua MODIS, NPP, 0.05 degrees, Global, Science Quality

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from NASA's Aqua Spacecraft. Measurements are gathered by the Moderate Resolution Imaging...

Chlorophyll-a, Aqua MODIS, NPP, 0.0125 degrees, East US, EXPERIMENTAL

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from NASA's Aqua Spacecraft. Measurements are gathered by the Moderate Resolution Imaging...

Chlorophyll-a, Aqua MODIS, NPP, 0.0125 degrees, West US, EXPERIMENTAL

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from NASA's Aqua Spacecraft. Measurements are gathered by the Moderate Resolution Imaging...

Evaluating the impact of sea surface temperature (SST) on spatial distribution of chlorophyll-a concentration in the East China Sea

Science.gov (United States)

Ji, Chenxu; Zhang, Yuanzhi; Cheng, Qiuming; Tsou, JinYeu; Jiang, Tingchen; Liang, X. San

2018-06-01

In this study, we analyze spatial and temporal sea surface temperature (SST) and chlorophylla (Chl-a) concentration in the East China Sea (ECS) during the period 2003-2016. Level 3 (4 km) monthly SST and Chl-a data from the Moderate Resolution Imaging Spectroradiometer Satellite (MODIS-Aqua) were reconstructed using the data interpolation empirical orthogonal function (DINEOF) method and used to evaluated the relationship between the two variables. The approaches employed included correlation analysis, regression analysis, and so forth. Our results show that certain strong oceanic SSTs affect Chl-a concentration, with particularly high correlation seen in the coastal area of Jiangsu and Zhejiang provinces. The mean temperature of the high correlated region was 18.67 °C. This finding may suggest that the SST has an important impact on the spatial distribution of Chl-a concentration in the ECS.

Cu toxicity on growth and chlorophyll-a of Chaetoceros sp.

Science.gov (United States)

Puspitasari, R.; Suratno; Purbonegoro, T.; Agustin, A. T.

2018-02-01

Phytoplankton is a primary producer in marine aquatic ecosystem. Their sensitivity to metal makes them important to study to predict the environmental impact of pollution. Copper is an essential nutrient for aquatic life as micronutrients on an organism but toxic at high levels. The focus of this study was to assess the toxicity of copper to Chaetoceros sp. on growth and chlorophyll-a content. The result shows that inhibition concentration (IC50) of copper on the microalgae, Chaetoceros sp. was 30.25 μg L-1. Growth of Chaetoceros sp. decreased 16.84% in 16 μg L-1 and 81.97% in 44 μg L-1. Chlorophyll-a content decreased dramatically at 44 μg L-1 compared to control. Increase of the cell size, deformation of cell wall and loss of setae were observed at higher concentration of copper.

Overview of remote sensing of chlorophyll flourescene in ocean waters

African Journals Online (AJOL)

Overview of remote sensing of chlorophyll flourescene in ocean waters. ... Besides empirical algorithms with the blue-green ratio, the algorithms based on ... between fluorescence and chlorophyll concentration and the red shift phenomena.

Patterns and Variability in Global Ocean Chlorophyll: Satellite Observations and Modeling

Science.gov (United States)

Gregg, Watson

2004-01-01

Recent analyses of SeaWiFS data have shown that global ocean chlorophyll has increased more than 4% since 1998. The North Pacific ocean basin has increased nearly 19%. These trend analyses follow earlier results showing decadal declines in global ocean chlorophyll and primary production. To understand the causes of these changes and trends we have applied the newly developed NASA Ocean Biogeochemical Assimilation Model (OBAM), which is driven in mechanistic fashion by surface winds, sea surface temperature, atmospheric iron deposition, sea ice, and surface irradiance. The model utilizes chlorophyll from SeaWiFS in a daily assimilation. The model has in place many of the climatic variables that can be expected to produce the changes observed in SeaWiFS data. This enables us to diagnose the model performance, the assimilation performance, and possible causes for the increase in chlorophyll. A full discussion of the changes and trends, possible causes, modeling approaches, and data assimilation will be the focus of the seminar.

VIIRSN Level-3 Standard Mapped Image, Chlorophyll a, Daily, 4km

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from the NPP -Suonomi Spacecraft. Measurements are gathered by the VIIRS instrument carried aboard the...

Chlorophyll Detection and Mapping of Shallow Water Impoundments Using Image Spectrometry

International Nuclear Information System (INIS)

Artigas, F.; Pechmann, I.; Marti, A.; Yao, N.; Pechmann, I.

2008-01-01

There exists a common perception that chlorophyll a concentrations in tidal coastal waters are unsuitable to be captured by remote sensing techniques because of high water turbidity. In this study, we use band index measurements to separate active chlorophyll pigments from other constituents in the water. Published single- and multiband spectral indices are used to establish a relationship between algal chlorophyll concentration and reflectance data. We find an index which is suitable to map chlorophyll gradients in the impoundments, ditches, and associated waterways of the Hackensack Meadow lands (NJ, USA). The resulting images clearly depict the spatial distribution of plant pigments and their relationship with the biological conditions of the waters in the estuary. Since these biological conditions are often determined by land usage, the methods in this paper provide a simple tool to address water quality management issues in fragmented urban estuaries.

Parameterization of vertical chlorophyll a in the Arctic Ocean: impact of the subsurface chlorophyll maximum on regional, seasonal, and annual primary production estimates

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M. Ardyna

2013-06-01

Full Text Available Predicting water-column phytoplankton biomass from near-surface measurements is a common approach in biological oceanography, particularly since the advent of satellite remote sensing of ocean color (OC. In the Arctic Ocean, deep subsurface chlorophyll maxima (SCMs that significantly contribute to primary production (PP are often observed. These are neither detected by ocean color sensors nor accounted for in the primary production models applied to the Arctic Ocean. Here, we assemble a large database of pan-Arctic observations (i.e., 5206 stations and develop an empirical model to estimate vertical chlorophyll a (Chl a according to (1 the shelf–offshore gradient delimited by the 50 m isobath, (2 seasonal variability along pre-bloom, post-bloom, and winter periods, and (3 regional differences across ten sub-Arctic and Arctic seas. Our detailed analysis of the dataset shows that, for the pre-bloom and winter periods, as well as for high surface Chl a concentration (Chl asurf; 0.7–30 mg m−3 throughout the open water period, the Chl a maximum is mainly located at or near the surface. Deep SCMs occur chiefly during the post-bloom period when Chl asurf is low (0–0.5 mg m−3. By applying our empirical model to annual Chl asurf time series, instead of the conventional method assuming vertically homogenous Chl a, we produce novel pan-Arctic PP estimates and associated uncertainties. Our results show that vertical variations in Chl a have a limited impact on annual depth-integrated PP. Small overestimates found when SCMs are shallow (i.e., pre-bloom, post-bloom > 0.7 mg m−3, and the winter period somehow compensate for the underestimates found when SCMs are deep (i.e., post-bloom −3. SCMs are, however, important seasonal features with a substantial impact on depth-integrated PP estimates, especially when surface nitrate is exhausted in the Arctic Ocean and where highly stratified and oligotrophic conditions prevail.

Chlorophyll-a, Aqua MODIS, NPP, 0.0125 degrees, Gulf of Mexico, EXPERIMENTAL

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from NASA's Aqua Spacecraft. Measurements are gathered by the Moderate Resolution Imaging...

Chlorophyll-a, Terra MODIS, OSU DB, 0.0125 degrees, West US, EXPERIMENTAL

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration data from NASA's Terra satellite. Measurements are gathered by the Moderate Resolution Imaging...

Synoptic relationships between surface Chlorophyll-a and diagnostic pigments specific to phytoplankton functional types

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M. Noguchi-Aita

2011-02-01

Full Text Available Error-quantified, synoptic-scale relationships between chlorophyll-a (Chl-a and phytoplankton pigment groups at the sea surface are presented. A total of ten pigment groups were considered to represent three Phytoplankton Size Classes (PSCs, micro-, nano- and picoplankton and seven Phytoplankton Functional Types (PFTs, i.e. diatoms, dinoflagellates, green algae, prymnesiophytes (haptophytes, pico-eukaryotes, prokaryotes and Prochlorococcus sp.. The observed relationships between Chl-a and PSCs/PFTs were well-defined at the global scale to show that a community shift of phytoplankton at the basin and global scales is reflected by a change in Chl-a of the total community. Thus, Chl-a of the total community can be used as an index of not only phytoplankton biomass but also of their community structure. Within these relationships, we also found non-monotonic variations with Chl-a for certain pico-sized phytoplankton (pico-eukaryotes, Prokaryotes and Prochlorococcus sp. and nano-sized phytoplankton (Green algae, prymnesiophytes. The relationships were quantified with a least-square fitting approach in order to enable an estimation of the PFTs from Chl-a where PFTs are expressed as a percentage of the total Chl-a. The estimated uncertainty of the relationships depends on both PFT and Chl-a concentration. Maximum uncertainty of 31.8% was found for diatoms at Chl-a = 0.49 mg m−3. However, the mean uncertainty of the relationships over all PFTs was 5.9% over the entire Chl-a range observed in situ (0.02 < Chl-a < 4.26 mg m−3. The relationships were applied to SeaWiFS satellite Chl-a data from 1998 to 2009 to show the global climatological fields of the surface distribution of PFTs. Results show that microplankton are present in the mid and high latitudes, constituting only ~10.9% of the entire phytoplankton community in the mean field for 1998–2009, in which diatoms explain ~7.5%. Nanoplankton are ubiquitous throughout the global surface oceans

A multi-band semi-analytical algorithm for estimating chlorophyll-a concentration in the Yellow River Estuary, China.

Science.gov (United States)

Chen, Jun; Quan, Wenting; Cui, Tingwei

2015-01-01

In this study, two sample semi-analytical algorithms and one new unified multi-band semi-analytical algorithm (UMSA) for estimating chlorophyll-a (Chla) concentration were constructed by specifying optimal wavelengths. The three sample semi-analytical algorithms, including the three-band semi-analytical algorithm (TSA), four-band semi-analytical algorithm (FSA), and UMSA algorithm, were calibrated and validated by the dataset collected in the Yellow River Estuary between September 1 and 10, 2009. By comparing of the accuracy of assessment of TSA, FSA, and UMSA algorithms, it was found that the UMSA algorithm had a superior performance in comparison with the two other algorithms, TSA and FSA. Using the UMSA algorithm in retrieving Chla concentration in the Yellow River Estuary decreased by 25.54% NRMSE (normalized root mean square error) when compared with the FSA algorithm, and 29.66% NRMSE in comparison with the TSA algorithm. These are very significant improvements upon previous methods. Additionally, the study revealed that the TSA and FSA algorithms are merely more specific forms of the UMSA algorithm. Owing to the special form of the UMSA algorithm, if the same bands were used for both the TSA and UMSA algorithms or FSA and UMSA algorithms, the UMSA algorithm would theoretically produce superior results in comparison with the TSA and FSA algorithms. Thus, good results may also be produced if the UMSA algorithm were to be applied for predicting Chla concentration for datasets of Gitelson et al. (2008) and Le et al. (2009).

Spatiotemporal chlorophyll-a dynamics on the Louisiana continental shelf derived from a dual satellite imagery algorithm

Science.gov (United States)

A monthly time series of remotely sensed chlorophyll-a (Chlars) over the Louisiana continental shelf (LCS) was developed and examined for its relationship to river discharge, nitrate concentration, total phosphorus concentration, photosynthetically available radiation (PAR), wind...

Effects of global climate change on chlorophyll-a concentrations in a tropical aquatic system during a cyanobacterial bloom: a microcosm study

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Meirielle Euripa Pádua de Moura

2017-05-01

Full Text Available Recent studies have investigated the impact of climate change on aquatic environments, and Chlorophyll-a (Chl-a concentration is a quick and reliable variable for monitoring such changes. This study evaluated the impact of rainfall frequency as a diluting agent and the effect of increased temperature on Chl-a concentrations in eutrophic environments during a bloom of cyanobacteria. This was based on the hypothesis that the concentration of Chl-a will be higher in treatments in which the rainfall frequency is not homogeneous and that warmer temperatures predicted due to climate change should favor higher concentrations of Chl-a. The experiment was designed to investigate three factors: temperature, precipitation and time. Temperature was tested with two treatment levels (22°C and the future temperature of 25°C. Precipitation was tested with four treatments (no precipitation, a homogeneous precipitation pattern, and two types of concentrated precipitation patterns. Experiments were run for 15 days, and Chl-a concentration was measured every five days in each of the temperature and precipitation treatments. The water used in the microcosms was collected from a eutrophic lake located in Central Brazil during a bloom of filamentous cyanobacteria (Geilterinema amphibium. Chl-a levels were high in all treatments. The higher temperature treatment showed increased Chl-a concentration (F=10.343; P=0.002; however, the extreme precipitation events did not significantly influence Chl-a concentrations (F=1.198; P=0.326. Therefore, the study demonstrates that future climatic conditions (projected to 2100, such as elevated temperatures, may affect the primary productivity of aquatic environments in tropical aquatic systems.

Chlorophyll-a, Orbview-2 SeaWiFS, 0.1 degrees, Global

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NASA GSFC Ocean Color Web distributes science-quality chlorophyll-a concentration data from the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) on the Orbview-2...

Regional variability among nonlinear chlorophyll-phosphorus relationships in lakes

Science.gov (United States)

Filstrup, Christopher T.; Wagner, Tyler; Soranno, Patricia A.; Stanley, Emily H.; Stow, Craig A.; Webster, Katherine E.; Downing, John A.

2014-01-01

The relationship between chlorophyll a (Chl a) and total phosphorus (TP) is a fundamental relationship in lakes that reflects multiple aspects of ecosystem function and is also used in the regulation and management of inland waters. The exact form of this relationship has substantial implications on its meaning and its use. We assembled a spatially extensive data set to examine whether nonlinear models are a better fit for Chl a—TP relationships than traditional log-linear models, whether there were regional differences in the form of the relationships, and, if so, which regional factors were related to these differences. We analyzed a data set from 2105 temperate lakes across 35 ecoregions by fitting and comparing two different nonlinear models and one log-linear model. The two nonlinear models fit the data better than the log-linear model. In addition, the parameters for the best-fitting model varied among regions: the maximum and lower Chl aasymptotes were positively and negatively related to percent regional pasture land use, respectively, and the rate at which chlorophyll increased with TP was negatively related to percent regional wetland cover. Lakes in regions with more pasture fields had higher maximum chlorophyll concentrations at high TP concentrations but lower minimum chlorophyll concentrations at low TP concentrations. Lakes in regions with less wetland cover showed a steeper Chl a—TP relationship than wetland-rich regions. Interpretation of Chl a—TP relationships depends on regional differences, and theory and management based on a monolithic relationship may be inaccurate.

Estimation of chlorophyll-a concentration in productive turbid waters using a Hyperspectral Imager for the Coastal Ocean-the Azov Sea case study

International Nuclear Information System (INIS)

Gitelson, Anatoly A; Gao Bocai; Li Rongrong; Berdnikov, Sergey; Saprygin, Vladislav

2011-01-01

We present here the results of chlorophyll-a (chl-a) concentration estimation using the red and near infrared (NIR) spectral bands of a Hyperspectral Imager for the Coastal Ocean (HICO) in productive turbid waters of the Azov Sea, Russia. During the data collection campaign in the summer of 2010 in Taganrog Bay and the Azov Sea, water samples were collected and concentrations of chl-a were measured analytically. The NIR-red models were tuned to optimize the spectral band selections and chl-a concentrations were retrieved from HICO data. The NIR-red three-band model with HICO-retrieved reflectances at wavelengths 684, 700, and 720 nm explained more than 85% of chl-a concentration variation in the range from 19.67 to 93.14 mg m -3 and was able to estimate chl-a with root mean square error below 10 mg m -3 . The results indicate the high potential of HICO data to estimate chl-a concentration in turbid productive (Case II) waters in real-time, which will be of immense value to scientists, natural resource managers, and decision makers involved in managing the inland and coastal aquatic ecosystems.

Evaluation of the physicochemical and chlorophyll-a conditions of a subtropical aquaculture in Lake Nasser area, Egypt

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Ahmed Mohamed El-Otify

2015-12-01

Full Text Available Water quality, nutritional characteristics and phytoplankton biomass in aquaculture of Oreochromis niloticus (L. simultaneously with its water source supply (Lake Nasser, were monthly investigated over a one-year period (July 2009–2010. Analysis of the results showed that application of fertilizers and environmental conditions appeared to be the principal factors influencing the spatio-temporal variations of water quality and productivity. The data obtained revealed differences between the fish pond and its water supply. Monthly fluctuations in the availability of the major nutrients reflected the occasional supply of the fish pond with different nutrients. Phytoplankton biomass in terms of chlorophyll-a concentrations were always of relatively higher values in the fish pond than those in Lake Nasser's water. Elevation of pH values and dissolved oxygen saturation levels appeared concomitantly with the increase of chlorophyll-a concentrations due to the phytoplankton photosynthetic assimilation activities. The observations of the present study highlighted the chlorophyll-a concentrations as an indicator of phytoplankton biomass reflecting water quality in the Nile tilapia aquaculture ecosystem.

Oceanic turbidity and chlorophyll as inferred from ERTS-1 observations

Science.gov (United States)

Curran, R. J.

1973-01-01

Spectral signatures of phytoplankton and other obscuring effects are considered in order to determine how to best use satellite data. The results of this study were then used to analyze the spectral data obtained from the ERTS-1 multispectral scanner (MSS). The analyzed satellite data were finally compared with surface ship measurements of chlorophyll concentration. It was found that the effects of water turbidity on the multispectral imagery can be discriminated by rationing the two shortest wavelength channels so that the effect of phytoplankton is enhanced.

Effect of automobile pollution on chlorophyll content of roadside urban trees

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M. Iqbal

2015-09-01

Full Text Available The effect of automobile pollution was determined on chlorophyll content of four different tree species viz. Azadirachta indica L., Conocarpus erectus L., Guiacum officinale L.and Eucalyptus sp. growing along the roads of the city.  Significant changes in the level of chlorophyll “a”, chlorophyll “b” and total chlorophyll “a+b” were found in the leaves of four tree species (A. indica, C. erectus, G.officinale and Eucalyptus sp. collected from polluted sites (Airport, Malir Halt, Quaidabad as compared to control site (Karachi University Campus. Lowest concentration of chlorophyll “a”, chlorophyll “b” and chlorophyll “a+b” was recorded in the leaf samples of all tree species collected from Quaidabad site when compared with the leaf samples collected from control site. The highest levels of chlorophyll pigment were recorded in all tree species leave samples collected from Karachi University Campus.  Similarly, better levels of chlorophyll “a”, chlorophyll “b” and total chlorophyll “a+b” was observed in all tree species growing at Airport site as compared to plants growing at Malir Halt and Quaidabad sites.  This study clearly indicated that the vehicular activities induced air pollution problem and affected on the level of chlorophyll pigments in trees which were exposed to road side pollution.

Cadmium toxicity affects chlorophyll a and b content, antioxidant enzyme activities and mineral nutrient accumulation in strawberry

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Ferhad Muradoglu

2015-01-01

Full Text Available BACKGROUND: Cadmium (Cd is well known as one of the most toxic metals affecting the environment and can severely restrict plant growth and development. In this study, Cd toxicities were studied in strawberry cv. Camarosa using pot experiment. Chlorophyll and malondialdehyde (MDA contents, catalase (CAT, superoxide dismutase (SOD, ascorbate peroxidase (APX activities and mineral nutrient concentrations were investigated in both roots and leaves of strawberry plant after exposure Cd. RESULTS: Cd content in both roots and leaves was increased with the application of increasing concentrations of Cd. We found higher Cd concentration in roots rather than in leaves. Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased Cd concentration treatments in both roots and leaves. SOD and CAT activities was also increased with the increase Cd concentrations. K, Mn and Mg concentrations were found higher in leaves than roots under Cd stress. In general, increased Cd treatments increased K, Mg, Fe, Ca, Cu and Zn concentration in both roots and leaves. Excessive Cd treatments reduced chlorophyll contents, increased antioxidant enzyme activities and changes in plant nutrition concentrations in both roots and leaves. CONCLUSION: The results presented in this work suggested that Cd treatments have negative effect on chlorophyll content and nearly decreased 30% of plant growth in strawberry. Strawberry roots accumulated higher Cd than leaves. We found that MDA and antioxidant enzyme (CAT, SOD and APX contents may have considered a good indicator in determining Cd tolerance in strawberry plant.

Quantifying the biological impact of surface ocean light attenuation by colored detrital matter in an ESM using a new optical parameterization

Science.gov (United States)

Kim, G. E.; Pradal, M.-A.; Gnanadesikan, A.

2015-08-01

Light attenuation by colored detrital material (CDM) was included in a fully coupled Earth system model (ESM). This study presents a modified parameterization for shortwave attenuation, which is an empirical relationship between 244 concurrent measurements of the diffuse attenuation coefficient for downwelling irradiance, chlorophyll concentration and light absorption by CDM. Two ESM model runs using this parameterization were conducted, with and without light absorption by CDM. The light absorption coefficient for CDM was prescribed as the average of annual composite MODIS Aqua satellite data from 2002 to 2013. Comparing results from the two model runs shows that changes in light limitation associated with the inclusion of CDM decoupled trends between surface biomass and nutrients. Increases in surface biomass were expected to accompany greater nutrient uptake and therefore diminish surface nutrients. Instead, surface chlorophyll, biomass and nutrients increased together. These changes can be attributed to the different impact of light limitation on surface productivity versus total productivity. Chlorophyll and biomass increased near the surface but decreased at greater depths when CDM was included. The net effect over the euphotic zone was less total biomass leading to higher nutrient concentrations. Similar results were found in a regional analysis of the oceans by biome, investigating the spatial variability of response to changes in light limitation using a single parameterization for the surface ocean. In coastal regions, surface chlorophyll increased by 35 % while total integrated phytoplankton biomass diminished by 18 %. The largest relative increases in modeled surface chlorophyll and biomass in the open ocean were found in the equatorial biomes, while the largest decreases in depth-integrated biomass and chlorophyll were found in the subpolar and polar biomes. This mismatch of surface and subsurface trends and their regional dependence was analyzed by

Why is the Ratio of Reflectivity Effective for Chlorophyll Estimation in the Lake Water?

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Kazuo Oki

2010-07-01

Full Text Available The reasons why it is effective to estimate the chlorophyll-a concentration with the ratio of spectral radiance reflectance at the red light region and near infrared regions were shown in theory using a two-flow model. It was found that all of the backscattering coefficients can consequently be ignored by using the ratio of spectral radiance reflectance, which is the ratio of the upward radiance to the downward irradiance, at the red light and near infrared regions. In other words, the ratio can be expressed by using only absorption coefficients, which are more stable for measurement than backscattering coefficients. In addition, the band selection is crucial for producing the band ratio when the chlorophyll-a concentration is estimated without the effects of backscattering. I conclude that the two wavelengths selected must be close, but one must be within the absorption range of chlorophyll-a, and the other must be outside of the absorption range of chlorophyll-a, in order to accurately estimate the chlorophyll-a concentration.

Evaluation of water quality by chlorophyll and dissolved oxygen

International Nuclear Information System (INIS)

Latif, Z.; Tasneem, M.A.; Javed, T.; Butt, S.; Fazil, M.; Ali, M.; Sajjad, M.I.

2002-01-01

This paper focuses on the impact of Chlorophyll and dissolved Oxygen on water quality. Kalar Kahar and Rawal lakes were selected for this research. A Spectrophotometer was used for determination of Chlorophyll a, Chlorophyll b, Chlorophyll c and Pheophytin pigment. Dissolved Oxygen was measured in situ, using dissolved oxygen meter. The gamma O/sup 18/ of dissolved Oxygen, like concentration, is affected primarily by three processes: air water gas exchange, respiration and photosynthesis; gamma O/sup 18/ is analyzed on isotopic ratio mass spectrometer, after extraction of dissolved Oxygen from water samples, followed by purification and conversion into CO/sub 2/. Rawal lake receives most of the water from precipitation during monsoon period and supplemented by light rains in December and January. This water is used throughout the year for drinking purposes in Rawalpindi city. The water samples were collected from 5, 7.5, and 10 meters of depth for seasonal studies of physiochemical and isotopic parameters of water and dissolved Oxygen. Optimum experimental conditions for delta O/sup 18/ analysis of dissolved Oxygen from aqueous samples were determined. Stratification of dissolved Oxygen was observed in Rawal Lake before rainy season in summer. The water quality deteriorates with depth, because the respiration exceeds the photosynthesis and gas exchange. The concentration and delta O/sup 18/ of dissolved Oxygen show no variation with depth in 1998 winter sampling. Kalar Kahar lake gets water from springs, which are recharged by local rains on the nearby mountains. It is a big lake, with shallow and uniform depth of nearly 1.5 meters. A lot of vegetation can be seen on the periphery of the lake. Algae have grown on the floor of the lake Water samples were collected from the corner with large amount of vegetation and from the center of the lake for dissolved Oxygen and Chlorophyll measurements. Chlorophyll result shows that Kalar Kahar Lake falls in Eutrophic category

TOXICITY OF INDUSTRIAL EFFLUENT ON TOTAL CHLOROPHYLL CONTENT OF CERTAIN AQUATIC MACROPHYTES

OpenAIRE

Singh Priti; Vishen Ashish; Wadhwani R; Pandey Y.N

2012-01-01

To assess the toxicity of industrial effluents on certain macrophytes, the total chlorophyll content of free floating, submerged and emergent macrophytes were estimated in concentrations of industrial effluents at varying exposure duration. The result revealed reduction in total chlorophyll content of exposed macrophytes at higher concentrations of industrial effluents on prolonged duration.

Effect of crude oil contamination on the chlorophyll content and morpho-anatomy of Cyperus brevifolius (Rottb.) Hassk.

Science.gov (United States)

Baruah, Plabita; Saikia, Rashmi Rekha; Baruah, Partha Pratim; Deka, Suresh

2014-11-01

Chlorophyll plays a pivotal role in the plant physiology and its productivity. Cultivation of plants in crude oil contaminated soil has a great impact on the synthesis of chlorophyll pigment. Morpho-anatomy of the experimental plant also shows structural deformation in higher concentrations. Keeping this in mind, a laboratory investigation has been carried out to study the effect of crude oil on chlorophyll content and morpho-anatomy of Cyperus brevifolius plant. Fifteen-day-old seedling of the plant was planted in different concentrations of the crude oil mixed soil (i.e., 10,000, 20,000, 30,000, 40,000, and 50,000 ppm). A control setup was also maintained without adding crude oil. Results were recorded after 6 months of plantation. Investigation revealed that there is a great impact of crude oil contamination on chlorophyll content of the leaves of the experimental plant. It also showed that chlorophyll a, chlorophyll b, and total chlorophyll content of leaves grown in different concentrations of crude oil were found to be lower than those of the control plant. Further, results also demonstrated that chlorophyll content was lowest in the treatment that received maximum dose of crude oil. It also showed that chlorophyll content was decreased with increased concentration of crude oil. Results also demonstrated that there was a reduction in plant shoot and root biomass with the increase of crude oil concentration. Results also revealed that the shoot biomass is higher than root biomass. Morphology and anatomy of the experimental plant also show structural deformation in higher concentrations. Accumulation of crude oil on the cuticle of the transverse section of the leaves and shoot forms a thick dark layer. Estimation of the level of pollution in an environment due to oil spill is possible by the in-depth study of the harmful effects of oil on the morphology and anatomy and chlorophyll content of the plants grown in that particular environment.

Isolation of chlorophyll a from spinach leaves

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E.D. Dikio

2008-08-01

Full Text Available An efficient method for separating chlorophyll a from spinach leaves by column chromatography and solvent extraction techniques has been developed. The purity and identity of the chlorophyll a have been confirmed by UV-Vis, IR and mass spectrometry. Yields from 100 g of freeze-dried spinach were 23 – 24 mg of chlorophyll a.

Gas exchange and chlorophyll a fluorescence parameters of ornamental bromeliads

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Karina Gonçalves da Silva

2017-10-01

Full Text Available Gas exchange and chlorophyll a fluorescence are widely used in physiological and ecological studies; however, few studies have used these techniques with ornamental plants. This study tested the potential contribution of gas exchange and chlorophyll a fluorescence to evaluate the water and nutrients uptake by the tank and root system of epiphyte bromeliad Guzmania lingulata. For this purpose, we conducted an experiment with different water regime and another with different concentrations of nitrogen. The experiments were: 1 - Watering: Control (application of water into Tank and Root, Tank (watering into Tank, Root (watering Root and Drought (water suspension during the 90 days of experimentation and 2 - Nitrogen: Plants fertilized with Hoagland and Arnon nutrient solution exclusively into Tank or Root with nitrogen concentrations of control and 2.62 or 5.34 mM N applied as urea. The Fv /Fm ratio allowed comparing the treatments between experiments, demonstrating that Root and Tank both have the capacity to maintain G. lingulata photosynthetic activity and growth, while Drought treatment (water suspension was the limiting factor for energy conversion efficiency of PSII. However, gas exchange was more permissive as a parameter for comparing treatments in the nitrogen experiment, providing important information about the general aspects of the photosynthetic process in the watering experiment. Both gas exchange and chlorophyll a fluorescence can support the evaluation of G. lingulata physiological status and can be useful tools in ornamental horticultural studies.

Chlorophyll b degradation by chlorophyll b reductase under high-light conditions.

Science.gov (United States)

Sato, Rei; Ito, Hisashi; Tanaka, Ayumi

2015-12-01

The light-harvesting chlorophyll a/b binding protein complex of photosystem II (LHCII) is the main antenna complex of photosystem II (PSII). Plants change their LHCII content depending on the light environment. Under high-light conditions, the content of LHCII should decrease because over-excitation damages the photosystem. Chlorophyll b is indispensable for accumulating LHCII, and chlorophyll b degradation induces LHCII degradation. Chlorophyll b degradation is initiated by chlorophyll b reductase (CBR). In land plants, NON-YELLOW COLORING 1 (NYC1) and NYC1-Like (NOL) are isozymes of CBR. We analyzed these mutants to determine their functions under high-light conditions. During high-light treatment, the chlorophyll a/b ratio was stable in the wild-type (WT) and nol plants, and the LHCII content decreased in WT plants. The chlorophyll a/b ratio decreased in the nyc1 and nyc1/nol plants, and a substantial degree of LHCII was retained in nyc1/nol plants after the high-light treatment. These results demonstrate that NYC1 degrades the chlorophyll b on LHCII under high-light conditions, thus decreasing the LHCII content. After the high-light treatment, the maximum quantum efficiency of the PSII photochemistry was lower in nyc1 and nyc1/nol plants than in WT and nol plants. A larger light-harvesting system would damage PSII in nyc1 and nyc1/nol plants. The fluorescence spectroscopy of the leaves indicated that photosystem I was also damaged by the excess LHCII in nyc1/nol plants. These observations suggest that chlorophyll b degradation by NYC1 is the initial reaction for the optimization of the light-harvesting capacity under high-light conditions.

Dissolved inorganic nutrients and chlorophyll on the narrow continental shelf of Eastern Brazil

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Gilmara Fernandes Eça

2014-03-01

Full Text Available The eastern Brazilian continental shelf is narrow and subject to the influence of a western boundary current system, presenting lower biological productivity than other regions. In this study, the distribution of water masses, dissolved inorganic nutrients, chlorophyll-a and total suspended solids (TSS on the inner shelf (< 35 m depth, between Itacaré and Canavieiras, eastern Brazil, is presented. Sampling surveys were carried out in March and August 2006 and March 2007. Tropical water (TW prevailed during March 2006 and August 2007 with the lower salinity waters (< 36 found in most samples taken in March 2007, reflecting the influence of continental outflow and rain in coastal waters. Low concentrations of dissolved inorganic nutrients and Chl-a found were typical of TW and results suggested that the inner shelf waters were depleted in dissolved inorganic nitrogen in August 2006 and March 2007, and in phosphate in March 2006, potentially affecting phytoplankton growth. Stratification of the water column was observed due to differences in dissolved nutrient concentrations, chlorophyll-a and TSS when comparing surface and bottom samples, possibly the result of a colder water intrusion and mixing on the bottom shelf and a deep chlorophyll maximum and/or sediment resuspension effect. Despite this stratification, oceanographic processes such as lateral mixing driven by the Brazil Current as well as a northward alongshore drift driven by winds and tides transporting Coastal Water can lead to an enhanced mixing of these waters promoting some heterogeneity in this oligotrophic environment.

Chlorophyll-a, Orbview-2 SeaWiFS, 0.0125 degrees, West US

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA CoastWatch distributes chlorophyll-a concentration images from the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) on the Orbview-2 satellite. Because data is...

CHANGES IN CHLOROPHYLL A FLUORENSCENCE AND PIGMENT RATIOS DURING DIFFERENT GROWTH PHASES OF A UNICELLULAR MARINE CHEATOSEROS (BACILLARIOPHYCEAE) IN BATCH CULTURE

Science.gov (United States)

Photosystem II reaction centers per cell decreased as the cultures began to decline. The degree of inactivation increased daily as the cell numbers continued to decrease. The concentration of chlorophyll a per cell and the ratio of the major accessory pigments to chlorophyll a (e...

Leaf reflectance-nitrogen-chlorophyll relations among three south Texas woody rangeland plant species

Science.gov (United States)

Gausman, H. W.; Everitt, J. H.; Escobar, D. E. (Principal Investigator)

1982-01-01

Annual variations in the nitrogen-chlorophyll leaf reflectance of hackberry, honey mesquite and live oak in south Texas, were compared. In spring, leaf reflectance at the 0.55 m wavelength and nitrogen (N) concentration was high but leaf chlorophyll (chl) concentrations were low. In summer, leaf reflectance and N-concentration were low but lead chl concentrations were high. Linear correlations for both spring and summer of leaf reflectance with N and chl concentration or deviations from linear regression were not statistically significant.

Toxicity of copper on the growth of marine microalgae Pavlova sp. and its chlorophyll-a

Science.gov (United States)

Purbonegoro, T.; Suratno; Puspitasari, R.; Husna, N. A.

2018-02-01

Marine microalgae is the primary producer at the base of the marine food chain. Their sensitivity to metal contamination provides important information for predicting the environmental impact of pollution. Toxicity testing using marine microalgae Pavlova sp. was carried out to assess the toxicity of copper on the growth and chlorophyll-a content. Results of this study show that adverse effects were observed by the increase of copper concentration. Cell number began to decrease at the lowest concentration (13 μg/L) and reduced drastically at 98 μg/L. Minimum cell number was observed at the highest concentration (890 μg/L). The inhibition concentration (IC50) value of copper for Pavlova sp. was 51.46 μg/L and at concentrations >29 μgL-1 the chlorophyll-a content decreased dramatically compared to the control. A variation in cell size and morphology was also observed at the higher concentration by the increase in the cell size and loss of setae compared to normal cells.

Effects of biocides on chlorophyll contents of detached basil leaves

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Titima Arunrangsi

2013-06-01

Full Text Available Herbicides and insecticides have been widely and intensively used in agricultural areas worldwide to enhance crop yield. However, many biocides cause serious environmental problems. In addition, the biocides may also have some effects on the treated agricultural crops. To study effects of biocides on chlorophyll content in detached basil leaves, 2,4-D dimethylamine salt (2,4 D-Amine, paraquat, carbosulfan, and azadirachtin, were chosen as representatives of biocide. After applying the chemicals to detached basil leaves overnight in darkness, chlorophyll contents were determined. Only treatment with 2,4 D-Amine resulted in reduction of chlorophyll contents significantly compared to treatment with deionized (DI water. In the case of paraquat and carbosulfan, chlorophyll contents were not significantly changed, while slightly higher chlorophyll contents, compared to DI water, after the treatment with azadirachtin, were observed. The results indicated that 2,4 D-Amine shows an ability to accelerate chlorophyll degradation, but azadirachtin helps to retard chlorophyll degradation, when each biocide is used at the concentration recommended by the manufacturer.

The AtCAO gene, encoding chlorophyll a oxygenase, is required for chlorophyll b synthesis in Arabidopsis thaliana

Science.gov (United States)

Espineda, Cromwell E.; Linford, Alicia S.; Devine, Domenica; Brusslan, Judy A.

1999-01-01

Chlorophyll b is synthesized from chlorophyll a and is found in the light-harvesting complexes of prochlorophytes, green algae, and both nonvascular and vascular plants. We have used conserved motifs from the chlorophyll a oxygenase (CAO) gene from Chlamydomonas reinhardtii to isolate a homologue from Arabidopsis thaliana. This gene, AtCAO, is mutated in both leaky and null chlorina1 alleles, and DNA sequence changes cosegregate with the mutant phenotype. AtCAO mRNA levels are higher in three different mutants that have reduced levels of chlorophyll b, suggesting that plants that do not have sufficient chlorophyll b up-regulate AtCAO gene expression. Additionally, AtCAO mRNA levels decrease in plants that are grown under dim-light conditions. We have also found that the six major Lhcb proteins do not accumulate in the null ch1-3 allele. PMID:10468639

Concentration and characterization of dissolved organic matter in the surface microlayer and subsurface water of the Bohai Sea, China

Science.gov (United States)

Chen, Yan; Yang, Gui-Peng; Wu, Guan-Wei; Gao, Xian-Chi; Xia, Qing-Yan

2013-01-01

A total of 19 sea-surface microlayer and corresponding subsurface samples collected from the Bohai Sea, China in April 2010 were analyzed for chlorophyll a, dissolved organic carbon (DOC) and its major compound classes including total dissolved carbohydrates (TDCHO, including monosaccharides, MCHO, and polysaccharides, PCHO) and total hydrolysable amino acids (THAA, including dissolved free, DFAA, and combined fraction, DCAA). The concentrations of DOC in the subsurface water ranged from 130.2 to 407.7 μM C, with an average of 225.9±75.4 μM C, while those in the surface microlayer varied between 140.1 and 330.9 μM C, with an average of 217.8±56.8 μM C. The concentrations of chlorophyll a, DOC, TDCHO and THAA in the microlayer were, respectively correlated with their subsurface water concentrations, implying that there was a strong exchange effect between the microlayer and subsurface water. The concentrations of DOC and TDCHO were negatively correlated with salinity, respectively, indicating that water mixing might play an important role in controlling the distribution of DOC and TDCHO in the water column. Major constituents of DCAA and DFAA present in the study area were glycine, alanine, glutamic acid, serine and histidine. Principal component analysis (PCA) was applied to examine the complex compositional differences that existed among the sampling sites. Our results showed that DFAA had higher mole percentages of glycine, valine and serine in the microlayer than in the subsurface water, while DCAA tended to have higher mole percentages of glutamic acid, aspartic acid, threonine, arginine, alanine, tyrosine, phenylalanine and leucine in the microlayer. The yields of TDCHO and THAA exhibited similar trends between the microlayer and subsurface water. Carbohydrate species displayed significant enrichment in the microlayer, whereas the DFAA and DCAA exhibited non-uniform enrichment in the microlayer.

Vegetation chlorophyll estimates in the Amazon from multi-angle MODIS observations and canopy reflectance model

Science.gov (United States)

Hilker, Thomas; Galvão, Lênio Soares; Aragão, Luiz E. O. C.; de Moura, Yhasmin M.; do Amaral, Cibele H.; Lyapustin, Alexei I.; Wu, Jin; Albert, Loren P.; Ferreira, Marciel José; Anderson, Liana O.; dos Santos, Victor A. H. F.; Prohaska, Neill; Tribuzy, Edgard; Barbosa Ceron, João Vitor; Saleska, Scott R.; Wang, Yujie; de Carvalho Gonçalves, José Francisco; de Oliveira Junior, Raimundo Cosme; Cardoso Rodrigues, João Victor Figueiredo; Garcia, Maquelle Neves

2017-06-01

As a preparatory study for future hyperspectral missions that can measure canopy chemistry, we introduce a novel approach to investigate whether multi-angle Moderate Resolution Imaging Spectroradiometer (MODIS) data can be used to generate a preliminary database with long-term estimates of chlorophyll. MODIS monthly chlorophyll estimates between 2000 and 2015, derived from a fully coupled canopy reflectance model (ProSAIL), were inspected for consistency with eddy covariance fluxes, tower-based hyperspectral images and chlorophyll measurements. MODIS chlorophyll estimates from the inverse model showed strong seasonal variations across two flux-tower sites in central and eastern Amazon. Marked increases in chlorophyll concentrations were observed during the early dry season. Remotely sensed chlorophyll concentrations were correlated to field measurements (r2 = 0.73 and r2 = 0.98) but the data deviated from the 1:1 line with root mean square errors (RMSE) ranging from 0.355 μg cm-2 (Tapajós tower) to 0.470 μg cm-2 (Manaus tower). The chlorophyll estimates were consistent with flux tower measurements of photosynthetically active radiation (PAR) and net ecosystem productivity (NEP). We also applied ProSAIL to mono-angle hyperspectral observations from a camera installed on a tower to scale modeled chlorophyll pigments to MODIS observations (r2 = 0.73). Chlorophyll pigment concentrations (ChlA+B) were correlated to changes in the amount of young and mature leaf area per month (0.59 ≤ r2 ≤ 0.64). Increases in MODIS observed ChlA+B were preceded by increased PAR during the dry season (0.61 ≤ r2 ≤ 0.62) and followed by changes in net carbon uptake. We conclude that, at these two sites, changes in LAI, coupled with changes in leaf chlorophyll, are comparable with seasonality of plant productivity. Our results allowed the preliminary development of a 15-year time series of chlorophyll estimates over the Amazon to support canopy chemistry studies using future

Estimation of chlorophyll-a concentration in estuarine waters: case study of the Pearl River estuary, South China Sea

International Nuclear Information System (INIS)

Zhang Yuanzhi; Lin Hui; Chen, Chuqun; Chen Liding; Zhang Bing; Gitelson, Anatoly A

2011-01-01

The objective of this work is to estimate chlorophyll-a (chl-a) concentration in the Pearl River estuary in China. To test the performance of algorithms for the estimation of the chl-a concentration in these productive turbid waters, the maximum band ratio (MBR) and near-infrared-red (NIR-red) models are used in this study. Specific focus is placed on (a) comparing the ability of the models to estimate chl-a in the range 1-12 mg m -3 , which is typical for coastal and estuarine waters, and (b) assessing the potential of the Moderate Resolution Imaging Spectrometer (MODIS) and Medium Resolution Imaging Spectrometer (MERIS) to estimate chl-a concentrations. Reflectance spectra and water samples were collected at 13 stations with chl-a ranging from 0.83 to 11.8 mg m -3 and total suspended matter from 9.9 to 21.5 g m -3 . A close relationship was found between chl-a concentration and total suspended matter concentration with the determining coefficient (R 2 ) above 0.89. The MBR calculated in the spectral bands of MODIS proved to be a good proxy for chl-a concentration (R 2 > 0.93). On the other hand, both the NIR-red three-band model, with wavebands around 665, 700, and 730 nm, and the NIR-red two-band model (with bands around 665 and 700 nm) explained more than 95% of the chl-a variation, and we were able to estimate chl-a concentrations with a root mean square error below 1 mg m -3 . The two- and three-band NIR-red models with MERIS spectral bands accounted for 93% of the chl-a variation. These findings imply that the extensive database of MODIS and MERIS images could be used to quantitatively monitor chl-a in the Pearl River estuary.

Estimation of chlorophyll-a concentration in estuarine waters: case study of the Pearl River estuary, South China Sea

Energy Technology Data Exchange (ETDEWEB)

Zhang Yuanzhi; Lin Hui [Institute of Space and Earth Information Science, Yuen Yuen Research Centre for Satellite Remote Sensing, Chinese University of Hong Kong, Shatin, N.T. (Hong Kong); Chen, Chuqun [South China Institute of Oceanology, Chinese Academy of Sciences, Guangzhou (China); Chen Liding [Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing (China); Zhang Bing [Center for Earth Observation and Digital Earth, Chinese Academy of Sciences, Beijing (China); Gitelson, Anatoly A, E-mail: yuanzhizhang@cuhk.edu.hk [Center for Advanced Land Management Information Technologies (CALMIT), School of Natural Resources, University of Nebraska-Lincoln (United States)

2011-04-15

The objective of this work is to estimate chlorophyll-a (chl-a) concentration in the Pearl River estuary in China. To test the performance of algorithms for the estimation of the chl-a concentration in these productive turbid waters, the maximum band ratio (MBR) and near-infrared-red (NIR-red) models are used in this study. Specific focus is placed on (a) comparing the ability of the models to estimate chl-a in the range 1-12 mg m{sup -3}, which is typical for coastal and estuarine waters, and (b) assessing the potential of the Moderate Resolution Imaging Spectrometer (MODIS) and Medium Resolution Imaging Spectrometer (MERIS) to estimate chl-a concentrations. Reflectance spectra and water samples were collected at 13 stations with chl-a ranging from 0.83 to 11.8 mg m{sup -3} and total suspended matter from 9.9 to 21.5 g m{sup -3}. A close relationship was found between chl-a concentration and total suspended matter concentration with the determining coefficient (R{sup 2}) above 0.89. The MBR calculated in the spectral bands of MODIS proved to be a good proxy for chl-a concentration (R{sup 2} > 0.93). On the other hand, both the NIR-red three-band model, with wavebands around 665, 700, and 730 nm, and the NIR-red two-band model (with bands around 665 and 700 nm) explained more than 95% of the chl-a variation, and we were able to estimate chl-a concentrations with a root mean square error below 1 mg m{sup -3}. The two- and three-band NIR-red models with MERIS spectral bands accounted for 93% of the chl-a variation. These findings imply that the extensive database of MODIS and MERIS images could be used to quantitatively monitor chl-a in the Pearl River estuary.

Completing the Feedback Loop: The Impact of Chlorophyll Data Assimilation on the Ocean State

Science.gov (United States)

Borovikov, Anna; Keppenne, Christian; Kovach, Robin

2015-01-01

In anticipation of the integration of a full biochemical model into the next generation GMAO coupled system, an intermediate solution has been implemented to estimate the penetration depth (1Kd_PAR) of ocean radiation based on the chlorophyll concentration. The chlorophyll is modeled as a tracer with sources-sinks coming from the assimilation of MODIS chlorophyll data. Two experiments were conducted with the coupled ocean-atmosphere model. In the first, climatological values of Kpar were used. In the second, retrieved daily chlorophyll concentrations were assimilated and Kd_PAR was derived according to Morel et al (2007). No other data was assimilated to isolate the effects of the time-evolving chlorophyll field. The daily MODIS Kd_PAR product was used to validate the skill of the penetration depth estimation and the MERRA-OCEAN re-analysis was used as a benchmark to study the sensitivity of the upper ocean heat content and vertical temperature distribution to the chlorophyll input. In the experiment with daily chlorophyll data assimilation, the penetration depth was estimated more accurately, especially in the tropics. As a result, the temperature bias of the model was reduced. A notably robust albeit small (2-5 percent) improvement was found across the equatorial Pacific ocean, which is a critical region for seasonal to inter-annual prediction.

Preliminary study of internal wave effects to chlorophyll distribution in the Lombok Strait and adjacent areas

Science.gov (United States)

Arvelyna, Yessy; Oshima, Masaki

2005-01-01

This paper studies the effect of internal wave in the Lombok Strait to chlorophyll distribution in the surrounded areas using ERS SAR, ASTER, SeaWiFS and AVHRR-NOAA images data during 1996-2004 periods. The observation results shows that the internal waves were propagated to the south and the north of strait and mostly occurred during transitional season from dry to wet and wet season (rainy season) between September to December when the layers are strongly stratified. Wavelet transform of image using Meyer wavelet analysis is applied for internal wave detection in ERS SAR and ASTER images, for symmetric extension of data at the image boundaries, to prevent discontinuities by a periodic wrapping of data in fast algorithm and space-saving code. Internal wave created elongated pattern in detail and approximation of image from level 2 to 5 and retained value between 2-4.59 times compared to sea surface, provided accuracy in classification over than 80%. In segmentation process, the Canny edge detector is applied on the approximation image at level two to derive internal wave signature in image. The proposed method can extract the internal wave signature, maintain the continuity of crest line while reduce small strikes from noise. The segmentation result, i.e. the length between crest and trough, is used to compute the internal wave induced current using Korteweg-de Vries (KdV) equation. On ERS SAR data contains surface signature of internal wave (2001/8/20), we calculated that internal wave propagation speed was 1.2 m/s and internal wave induced current was 0.56 m/s, respectively. From the observation of ERS SAR and SeaWiFS images data, we found out that the distribution of maximum chlorophyll area at southern coastline off Bali Island when strong internal wave induced current occurred in south of the Lombok Strait was distributed further to westward, i.e. from 9.25°-10.25°LS, 115°-116.25°SE to 8.8°-10.7°LS, 114.5°-116°SE, and surface chlorophyll concentration

Wind effects on coastal zone color scanner chlorophyll patterns in the U.S. Mid-Atlantic Bight during spring 1979

Science.gov (United States)

Eslinger, David L.; Iverson, Richard L.

1986-01-01

Coastal zone color scanner (CZCS) chlorophyll concentration increases in the Mid-Atlantic Bight were associated with high wind speeds in continental shelf waters during March and May 1979. Maximum spring CZCS chlorophyll concentrations occurred during April when the water column was not thermally stratified and were spatially and temporally associated with reductions in wind speed both in onshelf and in offshelf regions. Increased chlorophyll concentrations in offshelf waters were associated with high wind speeds during May when a deep chlorophyll maximum was present. Chlorophyll patchiness was observed on length scales typical of those controlled by biological processes during the April low-wind period but not during March or May when wind speeds were greater. The spring CZCS chlorophyll maximum in the southern portion of the Mid-Atlantic Bight occurred in response to a reduction in mixed layer depth caused by decreased wind speeds and not by increased water column stratification.

A Comparison between Local and Global Spaceborne Chlorophyll Indices in the St. Lawrence Estuary

Directory of Open Access Journals (Sweden)

Martin A. Montes-Hugo

2012-11-01

Full Text Available Spaceborne chlorophyll indices based on red fluorescence (wavelength = 680 nm and water leaving radiance (Lw in the visible spectrum (i.e., 400–700 nm were evaluated in the St Lawrence Estuary (SLE during September of 2011. Relationships between chlorophyll concentration (chl and fluorescence were constructed based on fluorescence line height (FLH measurements derived from a compact laser-based spectrofluorometer developed by ENEA (CASPER and using spectral bands corresponding to the satellite sensor MERIS (MEdium Resolution Imaging Spectrometer. Chlorophyll concentration as estimated from CASPER (chlCASPER was relatively high NE of the MTZ (upper Estuary, and nearby areas influenced by fronts or freshwater plumes derived from secondary rivers (lower estuary. These findings agree with historical shipboard measurements. In general, global chl products calculated from Lw had large biases (up to 27-fold overestimation and 50-fold underestimation with respect to chlCASPER values. This was attributed to the smaller interference of detritus (mineral + organic non-living particulates and chromophoric dissolved organic matter on chlCASPER estimates. We encourage the use of spectrofluorometry for developing and validating remote sensing models of chl in SLE waters and other coastal environments characterized by relatively low to moderate (<10 g·m−3 concentrations of detritus.

Detection of surface algal blooms using the newly developed algorithm surface algal bloom index (SABI)

Science.gov (United States)

Alawadi, Fahad

2010-10-01

Quantifying ocean colour properties has evolved over the past two decades from being able to merely detect their biological activity to the ability to estimate chlorophyll concentration using optical satellite sensors like MODIS and MERIS. The production of chlorophyll spatial distribution maps is a good indicator of plankton biomass (primary production) and is useful for the tracing of oceanographic currents, jets and blooms, including harmful algal blooms (HABs). Depending on the type of HABs involved and the environmental conditions, if their concentration rises above a critical threshold, it can impact the flora and fauna of the aquatic habitat through the introduction of the so called "red tide" phenomenon. The estimation of chlorophyll concentration is derived from quantifying the spectral relationship between the blue and the green bands reflected from the water column. This spectral relationship is employed in the standard ocean colour chlorophyll-a (Chlor-a) product, but is incapable of detecting certain macro-algal species that float near to or at the water surface in the form of dense filaments or mats. The ability to accurately identify algal formations that sometimes appear as oil spill look-alikes in satellite imagery, contributes towards the reduction of false-positive incidents arising from oil spill monitoring operations. Such algal formations that occur in relatively high concentrations may experience, as in land vegetation, what is known as the "red-edge" effect. This phenomena occurs at the highest reflectance slope between the maximum absorption in the red due to the surrounding ocean water and the maximum reflectance in the infra-red due to the photosynthetic pigments present in the surface algae. A new algorithm termed the surface algal bloom index (SABI), has been proposed to delineate the spatial distributions of floating micro-algal species like for example cyanobacteria or exposed inter-tidal vegetation like seagrass. This algorithm was

Chlorophyll-a, Orbview-2 SeaWiFS, 0.04167 degrees, Alaska, Science Quality

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NASA GSFC Ocean Color Web distributes science-quality chlorophyll-a concentration data from the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) on the Orbview-2...

The effects of different salt concentrations on growth and chlorophyll content of some pumpkin rootstocks

Directory of Open Access Journals (Sweden)

Köksal AYDİNŞAKİR

2015-12-01

Full Text Available This study was conducted to determine the effects of different salinity levels, (0.7, 4.0, 8.0, 12.0, 16.0 dS m-1 on some physiological parameters of Obez F1, Ferro F1, RS841 F1, which are used as rootstocks in watermelon cultivation. Salty irrigation water was obtained through mixing of NaCl and CaCl2 salt into tap water. When the plants were at the 3-4 leaf stage, different salinity levels were applied. Plants were harvested during the phase of florescence. The study was carried out using split plots in randomized complete block design while rootstocks are main plot, salinity levels are sub-plot with three replications. While the salinity level was increasing, the physiological parameters decreased in each three rootstocks. While the plant height changed between 14.4-107.1 cm, the plant leaf area varied between 152.0-2182.7 cm2. Chlorophyll-a, chlorophyll-b and total carotenoid values decreased as the salinity level increased. Excluding the maximum value obtained from control plot, the highest chlorophyll-a, chlorophyll-b, and total carotenoid values were obtained in Obez variety under 4.0 dS m-1 application with 14.8 mg l-1, 12.8 mg l-1 and 0.28 mg g-1 fw, respectively.

A database of chlorophyll a in Australian waters

Science.gov (United States)

Davies, Claire H.; Ajani, Penelope; Armbrecht, Linda; Atkins, Natalia; Baird, Mark E.; Beard, Jason; Bonham, Pru; Burford, Michele; Clementson, Lesley; Coad, Peter; Crawford, Christine; Dela-Cruz, Jocelyn; Doblin, Martina A.; Edgar, Steven; Eriksen, Ruth; Everett, Jason D.; Furnas, Miles; Harrison, Daniel P.; Hassler, Christel; Henschke, Natasha; Hoenner, Xavier; Ingleton, Tim; Jameson, Ian; Keesing, John; Leterme, Sophie C.; James McLaughlin, M.; Miller, Margaret; Moffatt, David; Moss, Andrew; Nayar, Sasi; Patten, Nicole L.; Patten, Renee; Pausina, Sarah A.; Proctor, Roger; Raes, Eric; Robb, Malcolm; Rothlisberg, Peter; Saeck, Emily A.; Scanes, Peter; Suthers, Iain M.; Swadling, Kerrie M.; Talbot, Samantha; Thompson, Peter; Thomson, Paul G.; Uribe-Palomino, Julian; van Ruth, Paul; Waite, Anya M.; Wright, Simon; Richardson, Anthony J.

2018-02-01

Chlorophyll a is the most commonly used indicator of phytoplankton biomass in the marine environment. It is relatively simple and cost effective to measure when compared to phytoplankton abundance and is thus routinely included in many surveys. Here we collate 173, 333 records of chlorophyll a collected since 1965 from Australian waters gathered from researchers on regular coastal monitoring surveys and ocean voyages into a single repository. This dataset includes the chlorophyll a values as measured from samples analysed using spectrophotometry, fluorometry and high performance liquid chromatography (HPLC). The Australian Chlorophyll a database is freely available through the Australian Ocean Data Network portal (https://portal.aodn.org.au/). These data can be used in isolation as an index of phytoplankton biomass or in combination with other data to provide insight into water quality, ecosystem state, and relationships with other trophic levels such as zooplankton or fish.

Vertical nutrient fluxes, turbulence and the distribution of chlorophyll a in the north-eastern North Sea

Science.gov (United States)

Bendtsen, Jørgen; Richardson, Katherine

2017-04-01

During summer the northern North Sea is characterized by nutrient rich bottom water masses and nutrient poor surface layers. This explains the distribution of chlorophyll a in the water column where a subsurface maximum, referred to as the deep chlorophyll maximum (DCM), often is present during the growth season. Vertical transport of nutrients between bottom water masses and the well lit surface layer stimulates phytoplankton growth and this generally explains the location of the DCM. However, a more specific understanding of the interplay between vertical transports, nutrient fluxes and phytoplankton abundance is required for identifying the nature of the vertical transport processes, e.g the role of advection versus vertical turbulent diffusion or the role of localized mixing associated with mesoscale eddies. We present results from the VERMIX study in the north-eastern North Sea where nutrients, chlorophyll a and turbulence profiles were measured along five north-south directed transects in July 2016. A high-resolution sampling program, with horizontal distances of 1-10 km between CTD-stations, resolved the horizontal gradients of chlorophyll a across the steep bottom slope from the relatively shallow central North Sea ( 50-80 m) towards the deep Norwegian Trench (>700 m). Low oxygen concentrations in the bottom water masses above the slope indicated enhanced biological production where vertical mixing would stimulate phytoplankton growth around the DCM. Measurements of variable fluorescence (Fv/Fm) showed elevated values in the DCM which demonstrates a higher potential for electron transport in the Photosystem II in the phytoplankton cells, i.e. an indication of nutrient-rich conditions favorable for phytoplankton production. Profiles of the vertical shear and microstructure of temperature and salinity were measured by a VMP-250 turbulence profiler and the vertical diffusion of nutrients was calculated from the estimated vertical turbulent diffusivity and the

Assimilation of remotely sensed chlorophyll fluorescence data into the land surface model CLM4

Science.gov (United States)

Wieneke, S.; Ahrends, H. E.; Rascher, U.; Schween, J.; Schickling, A.; Crewell, S.

2013-12-01

Photosynthesis is the most important exchange process of CO2 between the atmosphere and the land-surface. Therefore, the prediction of vegetation response to environmental conditions like increasing CO2 concentrations or plant stress is crucial for a reliable prediction of climate change. Photosynthesis is a complex physiological process that consists of numerous bio-physical sub-processes and chemical reactions. Spatial and temporal patterns of photosynthesis depend on dynamic plant-specific adaptation strategies to highly variable environmental conditions. Photosynthesis can be estimated using land-surface models, but, while state-of-the-art models often rely on Plant Functional Type (PFT) specific constants, they poorly simulate the dynamic adaptation of the physiological status of plant canopies in space and time. Remotely sensed sun-induced chlorophyll fluorescence (SICF) gives us now the possibility to estimate the diurnal dynamic vitality of the photosynthetic apparatus at both, the leaf and canopy levels. We installed within the framework of the Transregio32 project (www.tr32.de) automated hyperspectral fluorescence sensors at an agricultural site (winter wheat) in the Rur catchment area in West Germany at the end of July 2012. End of August, additional measurements of SIFC on nearby temperate grassland site (riparian meadow) and on a sugar beet field were performed. Spatial covering SICF data of the region were obtained during a measurement campaign using the newly developed air-borne hyperspectral sensor HyPlant on the 23 and 27 August 2012. SIFC data and data provided by eddy covariance measurements will be used to update certain model parameters that are normally set as constants. First model results demonstrate that the assimilation of SIFC into the Community Land Model 4 (CLM4) will result in a more realistic simulation of plant-specific adaptation strategies and therefore in a more realistic simulation of photosynthesis in space and time.

Seasonal and inter-annual variation in the chlorophyll content of three co-existing Sphagnum species exceeds the effect of solar UV reduction in a subarctic peatland.

Science.gov (United States)

Hyyryläinen, Anna; Rautio, Pasi; Turunen, Minna; Huttunen, Satu

2015-01-01

We measured chlorophyll (chl) concentration and chl a/b ratio in Sphagnum balticum, S. jensenii, and S. lindbergii, sampled after 7 and 8 years of ultraviolet-B (UVB) and temperature manipulation in an open field experiment in Finnish Lapland (68°N). We used plastic filters with different transmittance of UVB radiation to manipulate the environmental conditions. The plants were exposed to (1) attenuated UVB and increased temperature, (2) ambient UVB and increased temperature and (3) ambient conditions. Chlorophyll was extracted from the capitula of the mosses and the content and a/b ratio were measured spectrophotometrically. Seasonal variation of chlorophyll concentration in the mosses was species specific. Temperature increase to 0.5-1 °C and/or attenuation of solar UVB radiation to ca. one fifth of the ambient (on average 12 vs. 59 uW/cm(2)) had little effect on the chlorophyll concentration or its seasonal variation. In the dominant S. lindbergii, UVB attenuation under increased temperature led to a transient decrease in chlorophyll concentration. Altogether, species-specific patterns of seasonal chlorophyll variation in the studied Sphagna were more pronounced than temperature and UVB treatment effects.

Estimation of chlorophyll-a concentration in case II waters using MODIS and MERIS data-successes and challenges

International Nuclear Information System (INIS)

Moses, W J; Gitelson, A A; Berdnikov, S; Povazhnyy, V

2009-01-01

We present and discuss here the results of our work using MODIS (moderate resolution imaging spectroradiometer) and MERIS (medium resolution imaging spectrometer) satellite data to estimate the concentration of chlorophyll- a (chl-a) in reservoirs of the Dnieper River and the Sea of Azov, which are typical case II waters, i.e., turbid and productive. Our objective was to test the potential of satellite remote sensing as a tool for near-real-time monitoring of chl- a distribution in these water bodies. We tested the performance of a recently developed three-band model, and its special case, a two-band model, which use the reflectance at red and near-infrared wavelengths for the retrieval of chl- a concentration. The higher spatial resolution and the availability of a spectral band at around 708 nm with the MERIS data offered great promise for these models. We compared results from several different atmospheric correction procedures available for MODIS and MERIS data. No one particular procedure was consistently and systematically better than the rest. Nevertheless, even in the absence of a perfect atmospheric correction procedure, both the three-band and the two-band models showed promising results when compared with in situ chl- a measurements. The challenges and limitations involved in satellite remote monitoring of the chl- a distribution in turbid productive waters are discussed.

Chlorophyllase in Piper betle L. has a role in chlorophyll homeostasis and senescence dependent chlorophyll breakdown.

Science.gov (United States)

Gupta, Supriya; Gupta, Sanjay Mohan; Sane, Aniruddha P; Kumar, Nikhil

2012-06-01

Total chlorophyll content and chlorophyllase (chlorophyll-chlorophyllido hydrolase EC 3.1.1.14) activity in fresh leaves of Piper betle L. landrace KS was, respectively, twofold higher and eight fold lower than KV, showing negative correlation between chlorophyll and chlorophyllase activity. Specific chlorophyllase activity was nearly eightfold more in KV than KS. ORF of 918 nt was found in cloned putative chlorophyllase cDNAs from KV and KS. The gene was present as single copy in both the landraces. The encoded polypeptide of 306 amino acids differed only at two positions between the KV and KS; 203 (cysteine to tyrosine) and 301 (glutamine to glycine). Difference in chlorophyllase gene expression between KV and KS was evident in fresh and excised leaves. Up regulation of chlorophyllase gene by ABA and down regulation by BAP was observed in both the landraces; however, there was quantitative difference between KV and KS. Data suggests that chlorophyllase in P. betle is involved in chlorophyll homeostasis and chlorophyll loss during post harvest senescence.

Relationship between chlorophyll density and SPAD chlorophyll meter reading for Jerusalem artichoke (Helianthus tuberosus L.)

Science.gov (United States)

Chlorophyll is an indicator of crop health and productivity. Measuring chlorophyll is usually done directly and requires significant time and resources. Indirect measurement of chlorophyll density using a handheld portable chlorophyll meter can reduce time. However, this information is very limit...

Characteristic sediment and water column chlorophyll-a in the sea cucumber’s Paracaudina sp. habitat on the Kenjeran Water, Surabaya

Science.gov (United States)

Widianingsih, W.; Zaenuri, M.; Anggoro, S.; Kusumaningrum, H. P.; Hartati, R.

2018-03-01

The study of characteristic sediment and water column chlorophyll-a has an important role in the sea cucumber habitat. Sediment chlorophyll-a represents a productivity primer for the benthic community. This research has a purpose to investigate characteristic sediment and water column chlorophyll-a on the Kenjeran water, Surabaya. Sediment samples were collected by the ekman grab for analysis, grain size and nutrient. The sample for sediment chlorophyll-a was taken by core sampler. The water samples were taken with Nansen Bottles. According to the research result, the values of sediment chlorophyll-a at station 10, 11 and 12 were higher than the other stations. In contrast, the value of chlorophyll-a in the column water had almost the same value for each station. The sediment chlorophyll-a value on clay and silt sediment type was higher than the fine sand and coarse sediment type. The suitable habitat characteristic for Paracaudina sp. was clay and silt sediment with sediment chlorophyll concentration ranging from 347.82 mg·m-2 to 1135.52 mg·m-2.

The Validity Chlorophyll-a Estimation by Sun Induced Fluorescence in Estuarine Waters: An Analysis of Long-term (2003-2011) Water Quality Data from Tampa Bay, Florida (USA)

Science.gov (United States)

Moreno-Madrinan, Max Jacobo; Fischer, Andrew

2012-01-01

for some areas within the Bay, FLH can be a good predictor of chlorophyll-alpha concentration and hence a useful tool for the analysis of water quality. Overall, the results show a 106% increase in the validity of chlorophyll -a concentration estimates using FLH over the standard the blue-green OC3M algorithm. This analysis also illustrates that the correlations between FLH and in situ chlorophyll -a measurements increases with increasing water depth and distance of the monitoring sites from both the shore and structures. However, due to confounding factors related to the complexity of the estuarine system, a linear improvement in the FLH to chlorophyll ]a relationship was not clearly noted with increasing depth and distance from shore alone. Correlations of FLH with turbidity, nutrients (total nitrogen and total phosphorous) biological oxygen demand, salinity, sea surface temperature correlated positively with FLH concentrations, while dissolved oxygen and pH showed negative correlations. Principle component analyses are employed to further describe the relationships between the multivariate water quality parameters and the FLH product. The majority of sites with higher and very significant correlations (pless than 0.01) also showed high correlation values for nutrients, turbidity and biological oxygen demand. These sites were on average in greater than seven meters of water and over five kilometers from shore. A thorough understanding of the relationship between the MODIS FLH product and in situ water quality parameters will enhance our understanding of the accuracy MODIS fs global FLH algorithm and assist in optimizing its calibration for use in monitoring the quality of estuarine and coastal waters worldwide.

HPLC Analysis of Chlorophyll a, Chlorophyll b, and Beta-Carotene in Collard Greens: A Project for a Problem-Oriented Laboratory Course.

Science.gov (United States)

Silveira, Augustine, Jr.; And Others

1984-01-01

High performance liquid chromatography (HPLC) is used to separate and quantitate beta-carotene, chlorophyll a, and chlorophyll b originating from collard greens. Experimental procedures used and typical results obtained are discussed. (JN)

Chlorophyll-a Estimation Around the Antarctica Peninsula Using Satellite Algorithms: Hints from Field Water Leaving Reflectance.

Science.gov (United States)

Zeng, Chen; Xu, Huiping; Fischer, Andrew M

2016-12-07

Ocean color remote sensing significantly contributes to our understanding of phytoplankton distribution and abundance and primary productivity in the Southern Ocean (SO). However, the current SO in situ optical database is still insufficient and unevenly distributed. This limits the ability to produce robust and accurate measurements of satellite-based chlorophyll. Based on data collected on cruises around the Antarctica Peninsula (AP) on January 2014 and 2016, this research intends to enhance our knowledge of SO water and atmospheric optical characteristics and address satellite algorithm deficiency of ocean color products. We collected high resolution in situ water leaving reflectance (±1 nm band resolution), simultaneous in situ chlorophyll-a concentrations and satellite (MODIS and VIIRS) water leaving reflectance. Field samples show that clouds have a great impact on the visible green bands and are difficult to detect because NASA protocols apply the NIR band as a cloud contamination threshold. When compared to global case I water, water around the AP has lower water leaving reflectance and a narrower blue-green band ratio, which explains chlorophyll-a underestimation in high chlorophyll-a regions and overestimation in low chlorophyll-a regions. VIIRS shows higher spatial coverage and detection accuracy than MODIS. After coefficient improvement, VIIRS is able to predict chlorophyll a with 53% accuracy.

Chlorophyll-a Estimation Around the Antarctica Peninsula Using Satellite Algorithms: Hints from Field Water Leaving Reflectance

Directory of Open Access Journals (Sweden)

Chen Zeng

2016-12-01

Full Text Available Ocean color remote sensing significantly contributes to our understanding of phytoplankton distribution and abundance and primary productivity in the Southern Ocean (SO. However, the current SO in situ optical database is still insufficient and unevenly distributed. This limits the ability to produce robust and accurate measurements of satellite-based chlorophyll. Based on data collected on cruises around the Antarctica Peninsula (AP on January 2014 and 2016, this research intends to enhance our knowledge of SO water and atmospheric optical characteristics and address satellite algorithm deficiency of ocean color products. We collected high resolution in situ water leaving reflectance (±1 nm band resolution, simultaneous in situ chlorophyll-a concentrations and satellite (MODIS and VIIRS water leaving reflectance. Field samples show that clouds have a great impact on the visible green bands and are difficult to detect because NASA protocols apply the NIR band as a cloud contamination threshold. When compared to global case I water, water around the AP has lower water leaving reflectance and a narrower blue-green band ratio, which explains chlorophyll-a underestimation in high chlorophyll-a regions and overestimation in low chlorophyll-a regions. VIIRS shows higher spatial coverage and detection accuracy than MODIS. After coefficient improvement, VIIRS is able to predict chlorophyll a with 53% accuracy.

Portable chlorophyll meter (PCM-502) values are related to total chlorophyll concentration and photosynthetic capacity in papaya (Carica papaya L.)

Science.gov (United States)

This study was carried out to verify the practical use of the portable chlorophyll meter-PCM502 (PCM) in two papaya cultivars with contrasting green coloring of the leaf blade (‘Golden’: yellowish-green; ‘Solo’: dark green). The relationship was studied between the photosynthetic process and leaf n...

The Effects of Chlorophyll Assimilation on Carbon Fluxes in a Global Biogeochemical Model. [Technical Report Series on Global Modeling and Data Assimilation

Science.gov (United States)

Koster, Randal D. (Editor); Rousseaux, Cecile Severine; Gregg, Watson W.

2014-01-01

In this paper, we investigated whether the assimilation of remotely-sensed chlorophyll data can improve the estimates of air-sea carbon dioxide fluxes (FCO2). Using a global, established biogeochemical model (NASA Ocean Biogeochemical Model, NOBM) for the period 2003-2010, we found that the global FCO2 values produced in the free-run and after assimilation were within -0.6 mol C m(sup -2) y(sup -1) of the observations. The effect of satellite chlorophyll assimilation was assessed in 12 major oceanographic regions. The region with the highest bias was the North Atlantic. Here the model underestimated the fluxes by 1.4 mol C m(sup -2) y(sup -1) whereas all the other regions were within 1 mol C m(sup -2) y(sup -1) of the data. The FCO2 values were not strongly impacted by the assimilation, and the uncertainty in FCO2 was not decreased, despite the decrease in the uncertainty in chlorophyll concentration. Chlorophyll concentrations were within approximately 25% of the database in 7 out of the 12 regions, and the assimilation improved the chlorophyll concentration in the regions with the highest bias by 10-20%. These results suggest that the assimilation of chlorophyll data does not considerably improve FCO2 estimates and that other components of the carbon cycle play a role that could further improve our FCO2 estimates.

Manipulation of Origin of Life Molecules: Recognizing Single-Molecule Conformations in β-Carotene and Chlorophyll-a/β-Carotene Clusters

Energy Technology Data Exchange (ETDEWEB)

Ngo, Anh T.; Skeini, Timur [Nanoscale; amp, Quantum Phenomena Institute and Physics & amp, Astronomy Department, Ohio University, Athens, Ohio 45701, United States; Iancu, Violeta [Nanoscale; amp, Quantum Phenomena Institute and Physics & amp, Astronomy Department, Ohio University, Athens, Ohio 45701, United States; Redfern, Paul C.; Curtiss, Larry A.; Hla, Saw Wai [Nanoscale; amp, Quantum Phenomena Institute and Physics & amp, Astronomy Department, Ohio University, Athens, Ohio 45701, United States

2018-01-11

Carotenoids and chlorophyll are essential parts of plant leaves and are involved in photosynthesis, a vital biological process responsible for the origin of life on Earth. Here, we investigate how beta-carotene and chlorophyll-a form mixed molecular phases On a Au(111) surface using low-temperature scanning tunneling microscopy and molecular manipulation at the single-molecule level supported by density functional theory calculations. By isolating individual molecules from nanoscale molecular clusters with a scanning tunneling microscope tip, we are able to identify five beta-carotene conformations including a structure exhibiting a three-dimensional conformation. Furthermore, molecular resolution images enable direct visualization of beta-carotene/chlorophyll-a clsuters, with intimate structural details highlighting how they pair: beta-carotene preferentially positions next to chlorophyll-a and induces switching of chlorophyll-a from straight to several bent tail conformations in the molecular clusters.

Phosphorus, nitrogen and chlorophyll-a are significant factors controlling ciliate communities in summer in the northern Beibu Gulf, South China Sea.

Directory of Open Access Journals (Sweden)

Yibo Wang

Full Text Available Ciliates (protozoa are ubiquitous components of plankton community and play important roles in aquatic ecosystems in regards of their abundance, biomass, diversity and energy turnover. Based on the stratified samples collected from the northern Beibu Gulf in August 2011, species composition, abundance, biomass, diversity and spatial pattern of planktonic ciliates were studied. Furthermore the main environmental factors controlling ciliate communities were determined. A total of 101 species belonging to 44 genera and 7 orders (i.e., Oligotrichida, Haptorida, Euplotida, Sessilida, Pleurostomatida, Scuticociliatida and Tintinnida were identified. The variation of ciliate communities was significant at horizontal level, but that was not at vertical level. Based on cluster analysis, ciliate communities were divided into three main groups. Redundancy analysis (RDA revealed that Group A, existing in the waters with higher concentration of phosphorus and nitrogen, was dominated by Tintinnidium primitivum. Group B in the waters with lower temperature and chlorophyll-a concentration, was dominated by Leegaardiella ovalis. Group C, existing in the waters with higher temperature and chlorophyll-a concentration, was dominated by large Strombidium spp. and Mesodinium rubrum. Combining multiple analytic methods, our results strongly supported that phosphorus, nitrogen and chlorophyll-a were the most significant factors affecting the ciliate communities in the northern Beibu Gulf in summer. Concentration of phosphorus and nitrogen primarily influenced ciliate biomass, implying a potential impact of eutrophication on ciliate growth. The correlation with chlorophyll-a concentration, on one hand indicate the response of ciliates to the food availability, and on the other hand, the ciliates containing chloroplasts or endosymbionts may contribute greatly to the chlorophyll-a.

Pulse amplitude modulated chlorophyll fluorometer

Science.gov (United States)

Greenbaum, Elias; Wu, Jie

2015-12-29

Chlorophyll fluorometry may be used for detecting toxins in a sample because of changes in micro algae. A portable lab on a chip ("LOAC") based chlorophyll fluorometer may be used for toxin detection and environmental monitoring. In particular, the system may include a microfluidic pulse amplitude modulated ("PAM") chlorophyll fluorometer. The LOAC PAM chlorophyll fluorometer may analyze microalgae and cyanobacteria that grow naturally in source drinking water.

Lead pollution: effects on chlorophyll. [Phalaris canariensis, Lemna minor, graminaceae

Energy Technology Data Exchange (ETDEWEB)

Fiussello, N

1973-01-01

The emissions of motors are responsible for the high concentrations of lead in soil and plants near roads. In man, when the concentration of lead in blood exceeds the value of 30 ..mu..g/100 ml, is shown a decrease of haemaglobin and an increase of ALA urinary content. Since the early stages of chlorophyll biosynthetic pathway are similar if not identical with those leading to haem as far as protoporphyrin IX, it is interesting to ascertain if lead can specifically interfere in chlorophyll biosynthesis. In sand cultures with 200 p.p.m. of lead (the conc. in roadside soils), after 2 weeks, wheat shows a diminution of 16,5% in fresh weight 7,5% in dry weight and 6,5% in chlorophyll; Phalaris canariensis shows a diminution of 68% f.w., 41% d.w. and 39% chl. in comparison with the controls. A water-plant, Lemna minor, is more sensitive: the chlorophyll content, referred to dry weight, shows after a week a diminution of 32% and 55% with 10/sup -4/M and 10/sup -3/M lead nitrate. Lead in 200 p.p.m. conc. is surely poisonous against the tested plants but a specific action on chlorophyll synthesis could be accepted, at present, only for Lemna minor. It is possible that in wheat and in Phalaris a part of lead is bound in root-system, the more damaged, while in Lemna it can reach more easily the chloroplasts. At present a detectable increase of ALA, in plants treated with lead, has not been proved both in Graminaceae and in Lemna minor.

Chlorophyll modulation of mixed layer thermodynamics in a mixed-layer isopycnal general circulation model - An example from Arabian Sea and Equatorial Pacific

Digital Repository Service at National Institute of Oceanography (India)

Nakamoto, S.; PrasannaKumar, S.; Oberhuber, J.M.; Saito, H.; Muneyama, K.

and supported by quasi-steady upwelling. Remotely sensed chlorophyll pigment concentrations from the Coastal Zone Color Scanner (CZCS) are used to investigate the chlorophyll modulation of ocean mixed layer thermodynamics in a bulk mixed-layer model, embedded...

Spatiotemporal Variation in Mangrove Chlorophyll Concentration Using Landsat 8

Directory of Open Access Journals (Sweden)

Julio Pastor-Guzman

2015-11-01

Full Text Available There is a need to develop indicators of mangrove condition using remotely sensed data. However, remote estimation of leaf and canopy biochemical properties and vegetation condition remains challenging. In this paper, we (i tested the performance of selected hyperspectral and broad band indices to predict chlorophyll concentration (CC on mangrove leaves and (ii showed the potential of Landsat 8 for estimation of mangrove CC at the landscape level. Relative leaf CC and leaf spectral response were measured at 12 Elementary Sampling Units (ESU distributed along the northwest coast of the Yucatan Peninsula, Mexico. Linear regression models and coefficients of determination were computed to measure the association between CC and spectral response. At leaf level, the narrow band indices with the largest correlation with CC were Vogelmann indices and the MTCI (R2 > 0.5. Indices with spectral bands around the red edge (705–753 nm were more sensitive to mangrove leaf CC. At the ESU level Landsat 8 NDVI green, which uses the green band in its formulation explained most of the variation in CC (R2 > 0.8. Accuracy assessment between estimated CC and observed CC using the leave-one-out cross-validation (LOOCV method yielded a root mean squared error (RMSE = 15 mg·cm−2, and R2 = 0.703. CC maps showing the spatiotemporal variation of CC at landscape scale were created using the linear model. Our results indicate that Landsat 8 NDVI green can be employed to estimate CC in large mangrove areas where ground networks cannot be applied, and mapping techniques based on satellite data, are necessary. Furthermore, using upcoming technologies that will include two bands around the red edge such as Sentinel 2 will improve mangrove monitoring at higher spatial and temporal resolutions.

Chlorophyll a is a favorable substrate for Chlamydomonas Mg-dechelatase encoded by STAY-GREEN.

Science.gov (United States)

Matsuda, Kaori; Shimoda, Yousuke; Tanaka, Ayumi; Ito, Hisashi

2016-12-01

Mg removal from chlorophyll by Mg-dechelatase is the first step of chlorophyll degradation. Recent studies showed that in Arabidopsis, Stay Green (SGR) encodes Mg-dechelatase. Though the Escherichia coli expression system is advantageous for investigating the properties of Mg-dechelatase, Arabidopsis Mg-dechelatase is not successfully expressed in E. coli. Chlamydomonas reinhardtii SGR (CrSGR) has a long, hydrophilic tail, suggesting that active CrSGR can be expressed in E. coli. After the incubation of chlorophyll a with CrSGR expressed in E. coli, pheophytin a accumulated, indicating that active CrSGR was expressed in E. coli. Substrate specificity of CrSGR against chlorophyll b and an intermediate molecule of the chlorophyll b degradation pathway was examined. CrSGR exhibited no activity against chlorophyll b and low activity against 7-hydroxymethyl chlorophyll a, consistent with the fact that chlorophyll b is degraded only after conversion to chlorophyll a. CrSGR exhibited low activity against divinyl chlorophyll a and chlorophyll a', and no activity against chlorophyllide a, protochlorophyll a, chlorophyll c 2 , and Zn-chlorophyll a. These observations indicate that chlorophyll a is the most favorable substrate for CrSGR. When CrSGR was expressed in Arabidopsis cells, the chlorophyll content decreased, further confirming that SGR has Mg-dechelating activity in chloroplasts. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Instrumentation in Developing Chlorophyll Fluorescence Biosensing: A Review

Science.gov (United States)

Fernandez-Jaramillo, Arturo A.; Duarte-Galvan, Carlos; Contreras-Medina, Luis M.; Torres-Pacheco, Irineo; de J. Romero-Troncoso, Rene; Guevara-Gonzalez, Ramon G.; Millan-Almaraz, Jesus R.

2012-01-01

Chlorophyll fluorescence can be defined as the red and far-red light emitted by photosynthetic tissue when it is excited by a light source. This is an important phenomenon which permits investigators to obtain important information about the state of health of a photosynthetic sample. This article reviews the current state of the art knowledge regarding the design of new chlorophyll fluorescence sensing systems, providing appropriate information about processes, instrumentation and electronic devices. These types of systems and applications can be created to determine both comfort conditions and current problems within a given subject. The procedure to measure chlorophyll fluorescence is commonly split into two main parts; the first involves chlorophyll excitation, for which there are passive or active methods. The second part of the procedure is to closely measure the chlorophyll fluorescence response with specialized instrumentation systems. Such systems utilize several methods, each with different characteristics regarding to cost, resolution, ease of processing or portability. These methods for the most part include cameras, photodiodes and satellite images. PMID:23112686

Instrumentation in Developing Chlorophyll Fluorescence Biosensing: A Review

Directory of Open Access Journals (Sweden)

Jesus R. Millan-Almaraz

2012-08-01

Full Text Available Chlorophyll fluorescence can be defined as the red and far-red light emitted by photosynthetic tissue when it is excited by a light source. This is an important phenomenon which permits investigators to obtain important information about the state of health of a photosynthetic sample. This article reviews the current state of the art knowledge regarding the design of new chlorophyll fluorescence sensing systems, providing appropriate information about processes, instrumentation and electronic devices. These types of systems and applications can be created to determine both comfort conditions and current problems within a given subject. The procedure to measure chlorophyll fluorescence is commonly split into two main parts; the first involves chlorophyll excitation, for which there are passive or active methods. The second part of the procedure is to closely measure the chlorophyll fluorescence response with specialized instrumentation systems. Such systems utilize several methods, each with different characteristics regarding to cost, resolution, ease of processing or portability. These methods for the most part include cameras, photodiodes and satellite images.

Nutrient and chlorophyll relations in selected streams of the New England Coastal Basins in Massachusetts and New Hampshire, June-September 2001

Science.gov (United States)

Riskin, Melissa L.; Deacon, J.R.; Liebman, M.L.; Robinson, K.W.

2003-01-01

The U.S. Environmental Protection Agency is developing guidance to assist states with defining nutrient criteria for rivers and streams and to better describe nutrient-algal relations. As part of this effort, 13 wadeable stream sites were selected, primarily in eastern Massachusetts, for a nutrient-assessment study during the summer of 2001. The sites represent a range of water-quality impairment conditions (reference, moderately impaired, impaired) based on state regulatory agency assessments and previously assessed nitrogen, phosphorus, and dissolved-oxygen data. In addition, a combination of open- and closed-canopy locations were sampled at six of the sites to investigate the effect of sunlight on algal growth. Samples for nutrients and for chlorophyll I from phytoplankton and periphyton were collected at all stream sites. Total nitrogen (dissolved nitrite + nitrate + total ammonia + organic nitrogen) and total phosphorus (phosphorus in an unfiltered water sample) concentrations were lowest at reference sites and highest at impaired sites. There were statistically significant differences (p phytoplankton were not significantly different among site impairment designations. Concentrations of chlorophyll a from periphyton were highest at nutrient-impaired open-canopy sites. Chlorophyll a concentrations from periphyton samples were positively correlated with total nitrogen and total phosphorus at the open- and closed-canopy sites. Correlations were higher at open-canopy sites (p periphyton samples were observed between the open- and closed-canopy sites (p relations between total nitrogen and total phosphorus to periphyton chlorophyll a in wadeable streams from this study were quantified to present potential techniques for determining nutrient concentrations. Linear regression was used to estimate the total nitrogen and total phosphorus concentrations that corresponded to various chlorophyll a concentrations. On the basis of this relation, a median concentration for

Identification and classification of vertical chlorophyll patterns in the ...

African Journals Online (AJOL)

A type of artificial neural network called a self-organizing map (SOM) was then used on these four parameters to identify characteristic profiles. The analysis identified a continuum of chlorophyll patterns, from those with large surface peaks (>10 mg m-3) to those with smaller near-surface peaks (<2 mg m-3). The frequency of ...

Empirical model for chlorophyll-a determination in inland waters from the forthcoming Sentinel-2 and 3. Validation from HICO images

Directory of Open Access Journals (Sweden)

J. Delegido

2014-06-01

Full Text Available Chlorophyll-a concentration is one of the main indicators of inland waters quality. Using CHRIS/PROBA images and in situ data obtained in four lakes in Colombia and Spain, we obtained empirical models for the estimation of chlorophyll-a concentration, which can be directly applied to future images of MSI Sentinel-2 and OLCI Sentinel-3 sensors. The models, based on spectral band indices, were validated with data from the hyperspectral sensor HICO, onboard of the International Space Station.

Predictability and environmental drivers of chlorophyll fluctuations vary across different time scales and regions of the North Sea

Science.gov (United States)

Blauw, Anouk N.; Benincà, Elisa; Laane, Remi W. P. M.; Greenwood, Naomi; Huisman, Jef

2018-02-01

Phytoplankton concentrations display strong temporal variability at different time scales. Recent advances in automated moorings enable detailed investigation of this variability. In this study, we analyzed phytoplankton fluctuations at four automated mooring stations in the North Sea, which measured phytoplankton abundance (chlorophyll) and several environmental variables at a temporal resolution of 12-30 min for two to nine years. The stations differed in tidal range, water depth and freshwater influence. This allowed comparison of the predictability and environmental drivers of phytoplankton variability across different time scales and geographical regions. We analyzed the time series using wavelet analysis, cross correlations and generalized additive models to quantify the response of chlorophyll fluorescence to various environmental variables (tidal and meteorological variables, salinity, suspended particulate matter, nitrate and sea surface temperature). Hour-to-hour and day-to-day fluctuations in chlorophyll fluorescence were substantial, and mainly driven by sinking and vertical mixing of phytoplankton cells, horizontal transport of different water masses, and non-photochemical quenching of the fluorescence signal. At the macro-tidal stations, these short-term phytoplankton fluctuations were strongly driven by the tides. Along the Dutch coast, variation in salinity associated with the freshwater influence of the river Rhine played an important role, while in the central North Sea variation in weather conditions was a major determinant of phytoplankton variability. At time scales of weeks to months, solar irradiance, nutrient conditions and thermal stratification were the dominant drivers of changes in chlorophyll concentrations. These results show that the dominant drivers of phytoplankton fluctuations differ across marine environments and time scales. Moreover, our findings show that phytoplankton variability on hourly to daily time scales should not be

The effects of heavy metal ions on the chlorophyll content and cell membrane permeability of charophytes

International Nuclear Information System (INIS)

Fu Hualong; Chen Hao; Dong Bin; Qing Renwei

2001-01-01

The authors studied the effects of several heavy metal ions in different concentrations (Cd 2+ , Hg 2+ , Pb 2+ , Cr 6+ ) on the chlorophyll content and cell membrane permeability of Chara vulgaris L. It was discovered that the effects of heavy metal ions on the chlorophyll content and cell membrane permeability of Chara vulgaris L. changed with their different concentration. The trend was that the chlorophyll content and cell membrane permeability were decreased with the increase of the heavy metal ions. The degree of chlorophyll content affected was Cr 6+ , Cd 2+ , Hg 2+ , Pb 2+ , and that of cell membrane permeability affected was Cd 2+ , Cr 6+ , Hg 2+ , Pb 2+

Assessment of water pollution by airborne measurement of chlorophyll

Science.gov (United States)

Arvesen, J. C.; Weaver, E. C.; Millard, J. P.

1972-01-01

Remote measurement of chlorophyll concentrations to determine extent of water pollution is discussed. Construction and operation of radiometer to provide measurement capability are explained. Diagram of equipment is provided.

Effects of Cd2+ on chlorophyll content in flag and grain yield of wheats

International Nuclear Information System (INIS)

Zhu Zhiyong; Li Youjun; Liu Yingjie; Duan Youqiang; Li Qiang; Hao Yufen; Guo Jia

2011-01-01

A field experiment was conducted with wheat cultivars Luohan 6 and Yumai 18 to investigate the effects of Cd 2+ stress on chlorophyll contents in flag leaves, flag leave area, thousand kernel weight, kernel filling velocity and yield of wheat. Results indicated that, under low Cd 2+ stress (10 mg/kg), the average contents of chlorophyll a + b of Luohan 6 reduced by 1.6%, however, its average area of flag leave and yield increased by 3.8% and 1.6%, respectively. At the same time, the average content of chlorophyll a + b, area of flag leave yield of Yumai 18 reduced 8.0%, 9.6% and 5.4%. Under high Cd 2+ stress (100 mg/kg), the average contents of chlorophyll a + b, areas of flag leaves and yields of Luohan 6 and Yumai 18 reduced by 29.2% and 30.5%, 6.3% and 17.4%, 16.7% and 36.7%, respectively. The results demonstrated that Cd 2+ restrained synthesis and accumulation of chlorophyll and its components. This study even showed that within a range of Cd 2+ concentration could promote the growth of flag leaves, and it also had an equal positive effect on yield of wheat if the Cd 2+ concentration in grains were not out of limit. The growth of flag leave and yield of wheat would be limited when Cd 2+ concentration exceed that range. Overall, Yumai 18 bore more poison from Cd 2+ than Luohan 6. (authors)

Chlorophyll d: the puzzle resolved

DEFF Research Database (Denmark)

Larkum, Anthony W D; Kühl, Michael

2005-01-01

Chlorophyll a (Chl a) has always been regarded as the sole chlorophyll with a role in photochemical conversion in oxygen-evolving phototrophs, whereas chlorophyll d (Chl d), discovered in small quantities in red algae in 1943, was often regarded as an artefact of isolation. Now, as a result...... of discoveries over the past year, it has become clear that Chl d is the major chlorophyll of a free-living and widely distributed cyanobacterium that lives in light environments depleted in visible light and enhanced in infrared radiation. Moreover, Chl d not only has a light-harvesting role but might also...... replace Chl a in the special pair of chlorophylls in both reactions centers of photosynthesis. Udgivelsesdato: 2005-Aug...

Photosynthetic bark: use of chlorophyll absorption continuum index to estimate Boswellia papyrifera bark chlorophyll content

NARCIS (Netherlands)

Girma, A.; Skidmore, A.K.; Bie, de C.A.J.M.; Bongers, F.; Schlerf, M.

2013-01-01

Quantification of chlorophyll content provides useful insight into the physiological performance of plants. Several leaf chlorophyll estimation techniques, using hyperspectral instruments, are available. However, to our knowledge, a non-destructive bark chlorophyll estimation technique is not

Photosynthetic bark : use of chlorophyll absorption continuum index to estimate Boswellia papyrifera bark chlorophyll content

NARCIS (Netherlands)

Girma Gebrekidan, A.; Skidmore, A.K.; de Bie, C.A.J.M.; Bongers, Frans; Schlerf, Martin; Schlerf, M.

2013-01-01

Quantification of chlorophyll content provides useful insight into the physiological performance of plants. Several leaf chlorophyll estimation techniques, using hyperspectral instruments, are available. However, to our knowledge, a non-destructive bark chlorophyll estimation technique is not

Validation of MODIS FLH and In Situ Chlorophyll a from Tampa Bay, Florida (USA)

Science.gov (United States)

Fischer, Andrew; MorenoMadrinan, Max J.

2012-01-01

Satellite observation of phytoplankton concentration or chlorophyll-a (chla) is an important characteristic, critically integral to monitoring coastal water quality. However, the optical properties of estuarine and coastal waters are highly variable and complex and pose a great challenge for accurate analysis. Constituents such as suspended solids and dissolved organic matter and the overlapping and uncorrelated absorptions in the blue region of the spectrum renders the blue-green ratio algorithms for estimating chl-a inaccurate. Measurement of suninduced chlorophyll fluorescence, on the other hand, which utilizes the near infrared portion of the electromagnetic spectrum may, provide a better estimate of phytoplankton concentrations. While modelling and laboratory studies have illustrated both the utility and limitations of satellite algorithms based on the sun induced chlorophyll fluorescence signal, few have examined the empirical validity of these algorithms or compared their accuracy against bluegreen ratio algorithms . In an unprecedented analysis using a long term (2003-2011) in situ monitoring data set from Tampa Bay, Florida (USA), we assess the validity of the FLH product from the Moderate Resolution Imaging Spectrometer against a suite of water quality parameters taken in a variety of conditions throughout this large optically complex estuarine system. . Overall, the results show a 106% increase in the validity of chla concentration estimation using FLH over the standard chla estimate from the blue-green OC3M algorithm. Additionally, a systematic analysis of sampling sites throughout the bay is undertaken to understand how the FLH product responds to varying conditions in the estuary and correlations are conducted to see how the relationships between satellite FLH and in situ chlorophyll-a change with depth, distance from shore, from structures like bridges, and nutrient concentrations and turbidity. Such analysis illustrates that the correlations between

Liquid scintillation counting of chlorophyll

International Nuclear Information System (INIS)

Fric, F.; Horickova, B.; Haspel-Horvatovic, E.

1975-01-01

A precise and reproducible method of liquid scintillation counting was worked out for measuring the radioactivity of 14 C-labelled chlorophyll a and chlorophyll b solutions without previous bleaching. The spurious count rate caused by luminescence of the scintillant-chlorophyll system is eliminated by using a suitable scintillant and by measuring the radioactivity at 4 to 8 0 C after an appropriate time of dark adaptation. Bleaching of the chlorophyll solutions is necessary only for measuring of very low radioactivity. (author)

Two-photon excited fluorescence from higher electronic states of chlorophylls in photosynthetic antenna complexes a new approach to detect strong excitonic chlorophyll a/b coupling

CERN Document Server

Leupold, D; Ehlert, J; Irrgang, K D; Renger, G; Lokstein, H

2002-01-01

Stepwise two-photon excitation of chlorophyll a and b in the higher plant main light-harvesting complex (LHC II) and the minor complex CP29 (as well as in organic solution) with 100-fs pulses in the Q/sub y/ region results in a weak blue fluorescence. The dependence of the spectral shape of the blue fluorescence on excitation wavelength offers a new approach to elucidate the long-standing problem of the origin of spectral "chlorophyll forms" in pigment-protein complexes, in particular the characterization of chlorophyll a/b-heterodimers. As a first result we present evidence for the existence of strong chlorophyll a/b-interactions (excitonically coupled transitions at 650 and 680 nm) in LHC II at ambient temperature. In comparison with LHC II, the experiments with CP29 provide further evidence that the lowest energy chlorophyll a transition (at ~680 nm) is not excitonically coupled to chlorophyll b. (22 refs).

Characterization of chlorophyll binding to LIL3.

Science.gov (United States)

Mork-Jansson, Astrid Elisabeth; Eichacker, Lutz Andreas

2018-01-01

The light harvesting like protein 3 (LIL 3) from higher plants, has been linked to functions in chlorophyll and tocopherol biosynthesis, photo-protection and chlorophyll transfer. However, the binding of chlorophyll to LIL3 is unclear. We present a reconstitution protocol for chlorophyll binding to LIL3 in DDM micelles. It is shown in the absence of lipids and carotenoids that reconstitution of chlorophyll binding to in vitro expressed LIL3 requires pre-incubation of reaction partners at room temperature. We show chlorophyll a but not chlorophyll b binding to LIL3 at a molar ratio of 1:1. Neither dynamic light scattering nor native PAGE, enabled a discrimination between binding of chlorophyll a and/or b to LIL3.

Evaluation of nitrogen status and total chlorophyll in longkong (Aglaia dookkoo Griff. leaves under water stress using a chlorophyll meter

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Sdoodee, S.

2005-07-01

Full Text Available A chlorophyll meter (SPAD-502 was used to assess nitrogen status and total chlorophyll in longkong leaves, leaves from twelve of 10-year-old trees grown in the experimental plot at Prince of Songkla University, Songkhla province. The relationship between SPAD-502 meter reading and nitrogen status and total chlorophyll content analyzed in the laboratory was evaluated during 8 months (May-December 2003. It was found that the trend of the relationships in each month was similar. There was no significant differenceamong regression linears of all months. The data of 8 months showed that SPAD-reading and nitrogen content, and SPAD-reading and total chlorophyll content were related in a positive manner. They were Y = 0.19X+10.10, r = 0.76** (n = 240, and Y = 0.43X-7.89, r = 0.79** (n = 400, respectively. The SPAD-502 was then used to assess total nitrogen and total chlorophyll content during imposed water stress. Fifteen 4-yearold plants were grown in pots (each pot containing 50 kg soil volume. The experiment was arranged in acompletely randomized design with 3 treatments: (1 daily watering (2 once watering on day 7 (3 no watering with 5 replications during 14 days of the experimental period. Measurements showed a continuous decrease of SPAD-reading in the treatment of no watering. On day 14, a significant difference of SPAD- reading values between the treatment of daily watering and no watering was found. Then, the values of nitrogen content and total chlorophyll were assessed by using the linear regression equations. From the result, it is suggested that the measurement by chlorophyll meter is a rapid technique for the evaluation of total chlorophyll and nitrogen status in longkong leaves during water stress.

Integrating satellite retrieved leaf chlorophyll into land surface models for constraining simulations of water and carbon fluxes

KAUST Repository

Houborg, Rasmus

2013-07-01

In terrestrial biosphere models, key biochemical controls on carbon uptake by vegetation canopies are typically assigned fixed literature-based values for broad categories of vegetation types although in reality significant spatial and temporal variability exists. Satellite remote sensing can support modeling efforts by offering distributed information on important land surface characteristics, which would be very difficult to obtain otherwise. This study investigates the utility of satellite based retrievals of leaf chlorophyll for estimating leaf photosynthetic capacity and for constraining model simulations of water and carbon fluxes. © 2013 IEEE.

Simple heterogeneity parametrization for sea surface temperature and chlorophyll

Science.gov (United States)

Skákala, Jozef; Smyth, Timothy J.

2016-06-01

Using satellite maps this paper offers a complex analysis of chlorophyll & SST heterogeneity in the shelf seas around the southwest of the UK. The heterogeneity scaling follows a simple power law and is consequently parametrized by two parameters. It is shown that in most cases these two parameters vary only relatively little with time. The paper offers a detailed comparison of field heterogeneity between different regions. How much heterogeneity is in each region preserved in the annual median data is also determined. The paper explicitly demonstrates how one can use these results to calculate representative measurement area for in situ networks.

Assessing the Skill of Chlorophyll Forecasts: Latest Development and Challenges Ahead Using the Case of the Equatorial Pacific

Science.gov (United States)

Rousseaux, Cecile S.; Gregg, Watson W.

2018-01-01

Using a global ocean biogeochemical model combined with a forecast of physical oceanic and atmospheric variables from the NASA Global Modeling and Assimilation Office, we assess the skill of a chlorophyll concentrations forecast in the Equatorial Pacific for the period 2012-2015 with a focus on the forecast of the onset of the 2015 El Nino event. Using a series of retrospective 9-month hindcasts, we assess the uncertainties of the forecasted chlorophyll by comparing the monthly total chlorophyll concentration from the forecast with the corresponding monthly ocean chlorophyll data from the Suomi-National Polar-orbiting Partnership Visible Infrared Imaging Radiometer Suite (S-NPP VIIRS) satellite. The forecast was able to reproduce the phasing of the variability in chlorophyll concentration in the Equatorial Pacific, including the beginning of the 2015-2016 El Nino. The anomaly correlation coefficient (ACC) was significant (p less than 0.05) for forecast at 1-month (R=0.33), 8-month (R=0.42) and 9-month (R=0.41) lead times. The root mean square error (RMSE) increased from 0.0399 microgram chl L(exp -1) for the 1-month lead forecast to a maximum of 0.0472 microgram chl L(exp -1) for the 9-month lead forecast indicating that the forecast of the amplitude of chlorophyll concentration variability was getting worse. Forecasts with a 3-month lead time were on average the closest to the S-NPP VIIRS data (23% or 0.033 microgram chl L(exp -1)) while the forecast with a 9-month lead time were the furthest (31% or 0.042 microgram chl L(exp -1)). These results indicate the potential for forecasting chlorophyll concentration in this region but also highlights various deficiencies and suggestions for improvements to the current biogeochemical forecasting system. This system provides an initial basis for future applications including the effects of El Nino events on fisheries and other ocean resources given improvements identified in the analysis of these results.

Chlorophyll: The wonder pigment

Digital Repository Service at National Institute of Oceanography (India)

Bhat, S.R.

Chlorophyll, the green plant pigment, a 'real life force' of living beings, besides synthesizing food, is a great source of vitamins, minerals and other phytochemicals. Adding chlorophyll rich food to our diet fortifies our body against health...

A Structurally Simplified Hybrid Model of Genetic Algorithm and Support Vector Machine for Prediction of Chlorophyll a in Reservoirs

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Jieqiong Su

2015-04-01

Full Text Available With decreasing water availability as a result of climate change and human activities, analysis of the influential factors and variation trends of chlorophyll a has become important to prevent reservoir eutrophication and ensure water supply safety. In this paper, a structurally simplified hybrid model of the genetic algorithm (GA and the support vector machine (SVM was developed for the prediction of monthly concentration of chlorophyll a in the Miyun Reservoir of northern China over the period from 2000 to 2010. Based on the influence factor analysis, the four most relevant influence factors of chlorophyll a (i.e., total phosphorus, total nitrogen, permanganate index, and reservoir storage were extracted using the method of feature selection with the GA, which simplified the model structure, making it more practical and efficient for environmental management. The results showed that the developed simplified GA-SVM model could solve nonlinear problems of complex system, and was suitable for the simulation and prediction of chlorophyll a with better performance in accuracy and efficiency in the Miyun Reservoir.

Optimization and effects of different culture conditions on growth of Halomicronema hongdechloris – a filamentous cyanobacterium containing chlorophyll f

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Yaqiong eLi

2014-02-01

Full Text Available A chlorophyll f containing cyanobacterium, Halomicronema hongdechloris (H. hongdechloris was isolated from a stromatolite cyanobacterial community. However, the extremely slower growth rate of H. hongdechloris culture became a critical factor, hindering the research on this newly isolated cyanobacterium and the investigation of chlorophyll f-photosynthesis. Therefore, optimizing H. hongdechloris culture conditions has become an essential requirement for future research. This work investigated the effects of various culture conditions, essential nutrients and light environments to determine the optimal growth conditions for H. hongdechloris and the biosynthetic rate of chlorophyll f. Based on the total chlorophyll concentration, an optimal growth rate of 0.22 ± 0.02 day-1 (doubling time: 3.1 ± 0.3 days was observed when cells were grown under continuous illumination with far-red light with an intensity of 20 µE at 32°C in modified K+ES seawater (pH 8.0 with additional supplements of 11.75 mM NaNO3 and 0.15 mM K2HPO4. High performance liquid chromatography on H. hongdechloris pigments confirmed that chlorophyll a is the major chlorophyll and chlorophyll f constitutes approximately 10% of the total chlorophyll from cells grown under far-red light. Fluorescence confocal image analysis demonstrated changes of photosynthetic membranes and the distribution of photopigments in response to different light conditions. The total photosynthetic oxygen evolution yield per cell showed no changes under different light conditions, which confirms the involvement of chlorophyll f in oxygenic photosynthesis. The implications of the presence of chlorophyll f in H. hongdechloris and its relationship to light environment are discussed.

Prooxidative and antioxidative properties of β-carotene in chlorophyll and riboflavin photosensitized oil-in-water emulsions.

Science.gov (United States)

Park, JungWoo; Kim, Tae Soo; Kim, Mi-Ja; Lee, JaeHwan

2013-09-01

Effects of β-carotene on the oxidative stability of chlorophyll or riboflavin photosensitized oil-in-water (O/W) emulsions were determined by analysing the depleted headspace oxygen content, lipid hydroperoxides, fluorescence intensity, and headspace volatiles. As the concentration of β-carotene increased from 0 to 10, 100, and 1000 μM, the oxidative stability of samples containing chlorophylls decreased in a concentration-dependent manner under light, indicating that β-carotene acted as a prooxidant. However, in riboflavin photosensitized O/W emulsions, 100 and 1000 μM β-carotene inhibited lipid oxidation. Although β-carotene protected both photosensitisers in O/W emulsions, β-carotene displayed antioxidative or prooxidative properties, which depended on the polarity of the photosensitisers. The development of t-2-heptenal and 1-octen-3-ol increased greatly in chlorophyll sensitised O/W emulsions with an increase in β-carotene concentration, implying that more singlet oxygen oxidation occurred due to the higher levels of remaining chlorophylls. However, the content of t-2-heptenal and 1-octen-3-ol in the riboflavin sensitised O/W emulsions was not significantly correlated with the β-carotene concentration, which indicates that β-carotene in the lipid particles effectively quenched singlet oxygen. Copyright © 2013 Elsevier Ltd. All rights reserved.

Extraction of chlorophyll from pandan leaves using ethanol and mass transfer study

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Putra Meilana Dharma

2017-01-01

Full Text Available Green pigments are used in many industrial branches including food, drinks, soap and cosmetics. Chlorophyll can substitute synthetic dyes which may affect health. Chlorophyll can be extracted from pandan leaves; the pandan crop grows in many tropical areas. The effects of temperature, 30–70°C and agitation speed, 100–400 rpm on chlorophyll extraction from pandan leaves, using ethanol and the evaluation of mass transfer coefficient, using dimensionless analysis were investigated. The optimal conditions of extraction was obtained at 60°C and 300 rpm; the chlorophyll concentration was 107.1 mg L-1. The volumetric mass transfer coefficient increased with the temperature and agitation speed. Determination of volumetric mass transfer coefficient and dimensionless correlations are useful for further process development or industrial applications.

Water-Soluble Chlorophyll Protein (WSCP) Stably Binds Two or Four Chlorophylls.

Science.gov (United States)

Palm, Daniel M; Agostini, Alessandro; Tenzer, Stefan; Gloeckle, Barbara M; Werwie, Mara; Carbonera, Donatella; Paulsen, Harald

2017-03-28

Water-soluble chlorophyll proteins (WSCPs) of class IIa from Brassicaceae form tetrameric complexes containing one chlorophyll (Chl) per apoprotein but no carotenoids. The complexes are remarkably stable toward dissociation and protein denaturation even at 100 °C and extreme pH values, and the Chls are partially protected against photooxidation. There are several hypotheses that explain the biological role of WSCPs, one of them proposing that they function as a scavenger of Chls set free upon plant senescence or pathogen attack. The biochemical properties of WSCP described in this paper are consistent with the protein acting as an efficient and flexible Chl scavenger. At limiting Chl concentrations, the recombinant WSCP apoprotein binds substoichiometric amounts of Chl (two Chls per tetramer) to form complexes that are as stable toward thermal dissociation, denaturation, and photodamage as the fully pigmented ones. If more Chl is added, these two-Chl complexes can bind another two Chls to reach the fully pigmented state. The protection of WSCP Chls against photodamage has been attributed to the apoprotein serving as a diffusion barrier for oxygen, preventing its access to triplet excited Chls and, thus, the formation of singlet oxygen. By contrast, the sequential binding of Chls by WSCP suggests a partially open or at least flexible structure, raising the question of how WSCP photoprotects its Chls without the help of carotenoids.

Optimal leaf positions for chlorophyll meter measurement in rice

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Zhaofeng eYuan

2016-05-01

Full Text Available The Soil Plant Analysis Development (SPAD chlorophyll meter is one of the most commonly used diagnostic tools to measure crop nitrogen status. However, the measurement method of the meter could significantly affect the accuracy of the final estimation. Thus, this research was undertaken to develop a new methodology to optimize SPAD meter measurements in rice (Oryza sativa L.. A flatbed color scanner was used to map the dynamic chlorophyll distribution and irregular leaf shapes. Calculus algorithm was adopted to estimate the potential positions for SPAD meter measurement along the leaf blade. Data generated by the flatbed color scanner and SPAD meter were analysed simultaneously. The results suggested that a position 2/3 of the distance from the leaf base to the apex (2/3 position could represent the chlorophyll content of the entire leaf blade, as indicated by the relatively low variance of measurements at that positon. SPAD values based on di-positional leaves and the extracted chlorophyll a and b contents were compared. This comparison showed that the 2/3 position on the lower leaves tended to be more sensitive to changes in chlorophyll content. Finally, the 2/3 position and average SPAD values of the fourth fully expanded leaf from the top were compared with leaf nitrogen concentration. The results showed the 2/3 position on that leaf was most suitable for predicting the nitrogen status of rice. Based on these results, we recommend making SPAD measurements at the 2/3 position on the fourth fully expanded leaf from the top. The coupling of dynamic chlorophyll distribution and irregular leaf shapes information can provide a promising approach for the calibration of SPAD meter measurement, which can further benefit the in situ nitrogen management by providing reliable estimation of crops nitrogen nutrition status.

Superflux chlorophyll-a analysis: An assessment of variability in results introduced prior to fluorometric analysis. [chesapeake bay and shelf regions

Science.gov (United States)

Cibik, S. J.; Rutledge, C. K.; Robertson, C. N.

1981-01-01

Several experiments were undertaken to identify variability in results that came from procedural differences in the processing of chlorophyll samples prior to fluorometric analysis. T-tests on group means indicated that significant differences (alpha = 0.05) in phaeopigment a concentrations did result in samples not initially screened, but not in the chlorophyll a concentrations. Highly significant differences (alpha = 0.001) in group means were found in samples which were held in acetone after filtering as compared to unfiltered seawater samples held for the same period. No difference in results was found between the 24-hour extraction and samples which were processed immediately.

Chlorophyll as a biomarker for early disease diagnosis

Science.gov (United States)

Manzoor Atta, Babar; Saleem, M.; Ali, Hina; Arshad, Hafiz Muhammad Imran; Ahmed, M.

2018-06-01

The current study was designed to identify the stage for the diagnosis of disease before visible symptoms appeared. Fluorescence spectroscopy has been employed to identify disease signatures for its early diagnosis in rice plant leaves. Bacterial leaf blight (BLB) diseased and healthy leaf samples were collected from the rice fields in September, 2017 which were then used to record spectra using an excitation wavelength at 410 nm. The spectral range of emission was set from 420 to 800 nm which covers the blue–green and the chlorophyll bands. It was found that diseased leaves have a narrower ‘chlorophyll a’ band than healthy ones, and furthermore, that the emission band at 730 nm was either declined or depleted in the sample with high infection symptoms. In contrast, the blue–green region was observed to increase due to the emergence of disease. As the band intensity of chlorophyll decreases during infection, this decrease in chlorophyll content and increase in the blue–green spectral region could provide a new approach for predicting BLB at an early stage. The important finding was that the chlorophyll degradation and rise in the blue–green region take place in leaves with BLB or during BLB infection. Principal component analysis has been applied to spectral data which successfully separated diseased samples from healthy ones even with very small spectral variations.

Data and Dynamics Driven Approaches for Modelling and Forecasting the Red Sea Chlorophyll

KAUST Repository

Dreano, Denis

2017-05-31

Phytoplankton is at the basis of the marine food chain and therefore play a fundamental role in the ocean ecosystem. However, the large-scale phytoplankton dynamics of the Red Sea are not well understood yet, mainly due to the lack of historical in situ measurements. As a result, our knowledge in this area relies mostly on remotely-sensed observations and large-scale numerical marine ecosystem models. Models are very useful to identify the mechanisms driving the variations in chlorophyll concentration and have practical applications for fisheries operation and harmful algae blooms monitoring. Modelling approaches can be divided between physics- driven (dynamical) approaches, and data-driven (statistical) approaches. Dynamical models are based on a set of differential equations representing the transfer of energy and matter between different subsets of the biota, whereas statistical models identify relationships between variables based on statistical relations within the available data. The goal of this thesis is to develop, implement and test novel dynamical and statistical modelling approaches for studying and forecasting the variability of chlorophyll concentration in the Red Sea. These new models are evaluated in term of their ability to efficiently forecast and explain the regional chlorophyll variability. We also propose innovative synergistic strategies to combine data- and physics-driven approaches to further enhance chlorophyll forecasting capabilities and efficiency.

Influence of monsoonal winds on chlorophyll-α distribution in the Beibu Gulf.

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Chunyan Shen

Full Text Available The influence of seasonal, monsoonal winds on the temporal and spatial variability of chlorophyll-a (chl-a in the Beibu Gulf is studied based on long-term satellite data of sea surface winds, chl-a concentration and sea surface temperature (SST and in-situ observations for the years from 2002 to 2014. The analysis results indicated that under northeasterly monsoonal winds, chl-a concentrations were substantially elevated in most area of the Beibu Gulf, with a high chl-a concentration (>2 mg m-3 patch extending southwestward from the coastal water of the northeastern Gulf, consistent with the winter wind pattern. Meanwhile, the spatial distribution of high chl-a concentration is correlated with low SST in the northeastern Gulf. In the southern Gulf, there was generally low chl-a, except in the coastal waters southwest of Hainan Island. Here, the upwelling cold water prevails outside the mouth of the Beibu Gulf, driven by the southwesterly monsoonal winds and the runoff from the Changhua River, as implied by low observed SST. Correlation analysis indicated the chl-a concentration was strongly modulated by wind speed (r = 0.63, p0.7, p<0.001. Integrated analysis also showed that stratification is weak and mixing is strong in winter as affected by the high wind speed, which suggests that the wind-induced mixing is a dominant mechanism for entrainment of nutrients and the spatial distribution of chl-a in winter.

Indirect Estimations of Lentil Leaf and Plant N by SPAD Chlorophyll Meter

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Hossein Zakeri

2015-01-01

Full Text Available A Soil Plant Analysis Development (SPAD chlorophyll meter can be used to screen for leaf nitrogen (N concentration in breeding programs. Lentil (Lens culinaris L. cultivars were grown under varied N regimes, SPAD chlorophyll meter readings (SCMR were recorded from the cultivars leaves, and leaf N concentration was measured by combustion. Linear regression and the nonlinear Radial Basis Functions (RBF neural networks models were employed to estimate leaf N concentration (LNC based on the SCMR values. The closest estimates of LNC were obtained from the multivariate models in which the combination of plant age, leaf thickness, and SCMR was employed as the independent variable. In comparison, SCMR as the single independent variable in both models estimated less than 50% of LNC variations. The results showed significant effects of soil moisture and plant age on the association of LNC –SCMR as well as the relationship of LNC with plant N, grain yield, and days to maturity. However, the effect of cultivar on the measured variables was negligible. Although lentil N can be diagnosed by comparing SCMR values of the crop with those from a well-fertilized (N fixing plot, the results did not support using SPAD chlorophyll meter for screening lentil LNC.

Induction of chlorophyll chimeras and chlorophyll mutations in mungbean (Vigna radiata) cv. T44

International Nuclear Information System (INIS)

Singh, V.P.; Yadav, R.D.S.

1993-01-01

Uniform and healthy seeds of mungbean (Vigna radiata) cv. T44 were exposed to varying doses of gamma rays, ethyl methane sulphonate (EMS) and combination treatment of gamma rays with EMS. The data were recorded for seed germination, plant survival, frequency and spectrum of chlorophyll chimeras in M 1 and chlorophyll mutations in M 2 generation. Among all, the combination treatments were found most effective for inducing chlorophyll chimeras and chlorophyll mutations than the gamma rays or EMS alone. Of the mutants under reference, the albino, xantha and chlorina showed monogenic recessive while viridis exhibited digenic recessive inheritance. (author). 8 refs., 2 tabs

Growth and content of Spirulina platensis biomass chlorophyll cultivated at different values of light intensity and temperature using different nitrogen sources

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Eliane Dalva Godoy Danesi

2011-03-01

Full Text Available The effects of light intensity and temperature in S. platensis cultivation with potassium nitrate or urea as nitrogen source were investigated, as well as the biomass chlorophyll contents of this cyanobacteria, through the Response Surface Methodology. Experiments were performed at temperatures from 25 to 34.5ºC and light intensities from 15 to 69 µmol photons m-2 s-1, in mineral medium. In cultivations with both sources of nitrogen, KNO3 and urea, statistic evaluation through multiple regression, no interactions of such independent variables were detected in the results of the dependent variables maximum cell concentration, chlorophyll biomass contents, cell and chlorophyll productivities, as well as in the nitrogen-cell conversion factor. In cultivation performed with both sources of nitrogen, it was possible to obtain satisfactory adjustments to relate the dependent variables to the independent variables. The best results were achieved at temperature of 30ºC, at light intensity of 60 µmol photons m-2s-1, for cell growth, with cell productivity of approximately 95 mg L-1 d-1 in cultivations with urea. For the chlorophyll biomass content, the most adequate light intensity was 24 µmol photons m-2 s-1.

On extreme atmospheric and marine nitrogen fluxes and chlorophyll-a levels in the Kattegat Strait

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C. B. Hasager

2003-01-01

Full Text Available A retrospective analysis is carried out to investigate the importance of the vertical fluxes of nitrogen to the marine sea surface layer in which high chlorophyll a levels may cause blooms of harmful algae and subsequent turn over and oxygen depletion at the bottom of the sea. Typically nitrogen is the limiting factor for phytoplankton in the Kattegat Strait during summer periods (May to August and the major nitrogen inputs come from the atmosphere and deep-water entrainment. The extreme reoccurrence values of nitrogen from atmospheric wet and dry deposition and deep-water flux entrainments are calculated by the periodic maximum method and the results are successfully compared to a map of chlorophyll return periods based on in-situ observations. The one-year return of extreme atmospheric wet deposition is around 60 mg N m-2 day-1 and 30 mg N m-2 day-1 for deep-water entrainment. Atmospheric nitrogen dry deposition is insignificant in the context of algal blooms. At longer time-scales e.g. at 10-year return, the nitrogen deep-water entrainment is larger than the extreme of atmospheric wet deposition. This indicates that the pool of nitrogen released from the sea bottom by deep-water entrainment forced by high winds greatly exceeds the atmospheric pool of nitrogen washed out by precipitation. At the frontal zone of the Kattegat Strait and Skagerrak, the nitrogen deep-water entrainment is very high and this explains the high 10-year return chlorophyll level at 8 mg m-3 in the Kattegat Strait. In the southern part, the extreme chlorophyll level is only 4 mg m-3 according to the statistics of a multi-year time-series of water samples. The chlorophyll level varies greatly in time and space as documented by a series of SeaWiFS satellite maps (OC4v4 algorithm of chlorophyll ScanFish and buoy observations from an experimental period in the Kattegat Strait. It is recommended to sample in-situ chlorophyll observation collocated in time to the satellite

Chlorophyll-a Concentrations Affected by Discharge and Climate Conditions in Galveston Bay, Texas

Science.gov (United States)

Shen, X.; Gao, H.; Zhang, S.

2017-12-01

As the transition zones between river and ocean, estuaries face increasing pressure on their ecosystem health due to changes of freshwater quantity and quality—especially under the impacts of population growth, land use/land cover change, and climate change. Located at the northeast of Houston, Galveston Bay is of particular social economic importance for the State of Texas. Its freshwater inflow primarily arises from two rivers, the San Jacinto and the Trinity. While it is well recognized that Chlorophyll a (chla) concentration—an indicator of ecosystem health—is closely linked to river inflows and other environmental factors, no quantitative relationships have been established. The objectives of this study are to identify the spatial-temporal variations of chla, and to investigate the impacts of freshwater inflow and climatic factors on chla variability—so that prediction models can be developed for chla forecasting to provide scientific support for ecological management (in Galveston Bay). A 10-year validated remote sensing dataset is used in this analysis. The results show that chla has higher concentrations near the shoreline, as compared to the center of the bay. Temporally, chla tends to be higher in wet years than in dry years. Similarly, the seasonal fluctuations of chla are more significant during the wet months (from February to May) than the dry months (especially from August to December). Chla in the bay is primarily determined by discharge from the Trinity River in AMJ (April, May, June) and JAS (July, August, September). However, the driving factor is mainly water temperature in OND (October, November, December). In JFM (January, February, March), almost all of the factors (except discharge from the San Jacinto River) show significant correlation with chla. Based on these analyses, a chla prediction model is developed and tested. This result can provide scientific support for coastal environmental management in Galveston Bay, maintaining the

Evaluation of Hyperspectral Multi-Band Indices to Estimate Chlorophyll-A Concentration Using Field Spectral Measurements and Satellite Data in Dianshan Lake, China

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Linna Li

2013-04-01

Full Text Available Chlorophyll-a (Chl-a concentration is considered as a key indicator of the eutrophic status of inland water bodies. Various algorithms have been developed for estimating Chl-a in order to improve the accuracy of predictive models. The objective of this study is to assess the potential of hyperspectral multi-band indices to estimate the Chl-a concentration in Dianshan Lake, which is the largest lake in Shanghai, an international metropolis of China. Based on field spectral measurements and in-situ Chl-a concentration collected on 7–8 September 2010, hyperspectral multi-band indices were calibrated to estimate the Chl-a concentration with optimal wavelengths selected by model tuning. A three-band index accounts for 87.36% (R2 = 0.8736 of the Chl-a variation. A four-band index, which adds a wavelength in the near infrared (NIR region, results in a higher R2 (0.8997 by removing the absorption and backscattering effects of suspended solids. To test the applicability of the proposed indices for routinely monitoring of Chl-a in inland lakes, simulated Hyperion and real HJ-1A satellite data were selected to estimate the Chl-a concentration. The results show that the explanatory powers of these satellite hyperspectral multi-band indices are relatively high with R2 = 0.8559, 0.8945, 0.7969, and 0.8241 for simulated Hyperion and real HJ-1A satellite data, respectively. All of the results provide strong evidence that hyperspectral multi-band indices are promising and applicable to estimate Chl-a in eutrophic inland lakes.

Chlorophyll Extraction from Microalgae: A Review on the Process Engineering Aspects

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Aris Hosikian

2010-01-01

Full Text Available Chlorophyll is an essential compound in many everyday products. It is used not only as an additive in pharmaceutical and cosmetic products but also as a natural food colouring agent. Additionally, it has antioxidant and antimutagenic properties. This review discusses the process engineering of chlorophyll extraction from microalgae. Different chlorophyll extraction methods and chlorophyll purification techniques are evaluated. Our preliminary analysis suggests supercritical fluid extraction to be superior to organic solvent extraction. When compared to spectroscopic technique, high performance liquid chromatography was shown to be more accurate and sensitive for chlorophyll analysis. Finally, through CO2 capture and wastewater treatment, microalgae cultivation process was shown to have strong potential for mitigation of environmental impacts.

On extreme atmospheric and marine nitrogen fluxes and chlorophyll-a levels in the Kattegat Strait

DEFF Research Database (Denmark)

Hasager, C.B.; Carstensen, J.; Ellermann, T.

2003-01-01

A retrospective analysis is carried out to investigate the importance of the vertical fluxes of nitrogen to the marine sea surface layer in which high chlorophyll a levels may cause blooms of harmful algae and subsequent turn over and oxygen depletion at the bottom of the sea. Typically nitrogen ...

Study of 660 nm laser-induced photoluminescence of chlorophyll-a and its applications

International Nuclear Information System (INIS)

Song, Y; Zhang, D X; Zhang, H J

2007-01-01

Based on the phenomenon of chlorophyll a photoluminescence, this paper introduces a new method to measure the chlorophyll a content, using 660nm laser diode as a new kind of light source to stimulate fluorescence as well as combining a fiber and spectrum technique. We analyze the characteristics of laser-induced fluorescence spectrum of chlorophyll a and then put forward the new method using two parameters, the relative fluorescence intensity and fluorescence intensity ratio F685/F735, to measure the chlorophyll a content in the water and green leaves respectively. The experimental results indicate that it is completely feasible to give a visual judgment for chlorophyll a content, according to the fluorescence emission spectrum of chlorophyll a. Subsequently, it is verified by three kinds of typical applications. All of these provide a new kind of light source to develop the chlorophyll a fluorometry and further give a technical foundation of on-spot monitoring the chlorophyll a content in the ocean or in green leaves

Mutagenic effects of gamma rays and EMS on frequency and spectrum of chlorophyll mutations in urdbean (Vigna mungo (L.) Hepper)

International Nuclear Information System (INIS)

Usharani, K.S.; Ananda Kumar, C.R.

2015-01-01

Chlorophyll mutations act as a significant index in the judgment of induced genetic variations in mutagen treated populations. Different types of chlorophyll mutation have been observed in various crop plants. In the current study, the effect of different concentrations (40 kR, 50 kR and 60 kR) of gamma rays, Ethyl Methane Sulphonate (50 mM, 60 mM and 70 mM) in single and combination dose/concentration on the frequency and spectrum of chlorophyll mutation and the effect of VBN 4 urdbean variety to such irradiation dose was observed. Results showed induction of broad spectrum of chlorophyll mutations which included albina, xantha, chlorina and viridis. Among these chlorina type was predominant in all the mutagenic treatments. The albina type of chlorophyll mutants occurred very rarely and was found only at 60 mM of EMS treatment and at 40 kR + 50 mM, 60 kR + 70 mM of combination treatments. Based on the chlorophyll mutation frequency, gamma rays were most effective followed by EMS and combination of treatments. (author)

Dinitrogen fixation in a unicellular chlorophyll d-containing cyanobacterium

NARCIS (Netherlands)

Pfreundt, U.; Stal, L.J.; Voss, B.; Hess, W.R.

2012-01-01

Marine cyanobacteria of the genus Acaryochloris are the only known organisms that use chlorophyll d as a photosynthetic pigment. However, based on chemical sediment analyses, chlorophyll d has been recognized to be widespread in oceanic and lacustrine environments. Therefore it is highly relevant to

Impact of chlorophyll bias on the tropical Pacific mean climate in an earth system model

Science.gov (United States)

Lim, Hyung-Gyu; Park, Jong-Yeon; Kug, Jong-Seong

2017-12-01

Climate modeling groups nowadays develop earth system models (ESMs) by incorporating biogeochemical processes in their climate models. The ESMs, however, often show substantial bias in simulated marine biogeochemistry which can potentially introduce an undesirable bias in physical ocean fields through biogeophysical interactions. This study examines how and how much the chlorophyll bias in a state-of-the-art ESM affects the mean and seasonal cycle of tropical Pacific sea-surface temperature (SST). The ESM used in the present study shows a sizeable positive bias in the simulated tropical chlorophyll. We found that the correction of the chlorophyll bias can reduce the ESM's intrinsic cold SST mean bias in the equatorial Pacific. The biologically-induced cold SST bias is strongly affected by seasonally-dependent air-sea coupling strength. In addition, the correction of chlorophyll bias can improve the annual cycle of SST by up to 25%. This result suggests a possible modeling approach in understanding the two-way interactions between physical and chlorophyll biases by biogeophysical effects.

Realization of a four-step molecular switch in scanning tunneling microscope manipulation of single chlorophyll-a molecules

Science.gov (United States)

Iancu, Violeta; Hla, Saw-Wai

2006-01-01

Single chlorophyll-a molecules, a vital resource for the sustenance of life on Earth, have been investigated by using scanning tunneling microscope manipulation and spectroscopy on a gold substrate at 4.6 K. Chlorophyll-a binds on Au(111) via its porphyrin unit while the phytyl-chain is elevated from the surface by the support of four CH3 groups. By injecting tunneling electrons from the scanning tunneling microscope tip, we are able to bend the phytyl-chain, which enables the switching of four molecular conformations in a controlled manner. Statistical analyses and structural calculations reveal that all reversible switching mechanisms are initiated by a single tunneling-electron energy-transfer process, which induces bond rotation within the phytyl-chain. PMID:16954201

Coincident patterns of waste water suspended solids reduction, water transparency increase and chlorophyll decline in Narragansett Bay.

Science.gov (United States)

Borkman, David G; Smayda, Theodore J

2016-06-15

Dramatic changes occurred in Narragansett Bay during the 1980s: water clarity increased, while phytoplankton abundance and chlorophyll concentration decreased. We examine how changes in total suspended solids (TSS) loading from wastewater treatment plants may have influenced this decline in phytoplankton chlorophyll. TSS loading, light and phytoplankton observations were compiled and a light- and temperature-dependent Skeletonema-based phytoplankton growth model was applied to evaluate chlorophyll supported by TSS nitrogen during 1983-1995. TSS loading declined 75% from ~0.60×10(6)kgmonth(-1) to ~0.15×10(6)kgmonth(-1) during 1983-1995. Model results indicate that nitrogen reduction related to TSS reduction was minor and explained a small fraction (~15%) of the long-term chlorophyll decline. The decline in NBay TSS loading appears to have increased water clarity and in situ irradiance and contributed to the long-term chlorophyll decline by inducing a physiological response of a ~20% reduction in chlorophyll per cell. Copyright © 2016 Elsevier Ltd. All rights reserved.

Impact of measurement uncertainties on determination of chlorophyll-specific absorption coefficient for marine phytoplankton

OpenAIRE

McKee, D.; Röttgers, R.; Neukermans, G.; Calzado, V.S.; Trees, C.; Ampolo-Rella, M.; Neil, C.; Cunningham, A.

2014-01-01

Understanding variability in the chlorophyll-specific absorption of marine phytoplankton, aph*Chl (λ), is essential for primary production modelling, calculation of underwater light field characteristics, and development of algorithms for remote sensing of chlorophyll concentrations. Previous field and laboratory studies have demonstrated significant apparent variability in aph*Chl (λ) for natural samples and algal cultures. However, the potential impact of measurement uncertain...

Relationship between photosynthetic pigments and chlorophyll fluorescence in soybean under varying phosphorus nutrition at ambient and elevated CO2

Science.gov (United States)

Photosynthetic pigments such as chlorophyll (Chl) a, Chl b and carotenoids concentration, and chlorophyll fluorescence (CF) have widely been used as indicators of stress and photosynthetic performance in plants. Although photosynthetic pigments and CF are partly interdependent due to absorption and ...

Detection of Glycoalkaloids and Chlorophyll in Potatoes (Solanum tuberosum L.) by Hyperspectral Imaging

DEFF Research Database (Denmark)

Kjær, Anders; Nielsen, Glenn; Stærke, Søren

2017-01-01

The purpose of the study was to investigate the use of hyperspectral imaging (HSI) to detect and quantify chlorophyll (Chl) and total glycoalkaloid concentrations (TGA) in potatoes. To create a set of tubers with different concentrations of Chl and TGA, potatoes of four varieties were wounded...

Chlorophyll-a Algorithms for Oligotrophic Oceans: A Novel Approach Based on Three-Band Reflectance Difference

Science.gov (United States)

Hu, Chuanmin; Lee, Zhongping; Franz, Bryan

2011-01-01

A new empirical algorithm is proposed to estimate surface chlorophyll-a concentrations (Chl) in the global ocean for Chl less than or equal to 0.25 milligrams per cubic meters (approximately 77% of the global ocean area). The algorithm is based on a color index (CI), defined as the difference between remote sensing reflectance (R(sub rs), sr(sup -1) in the green and a reference formed linearly between R(sub rs) in the blue and red. For low Chl waters, in situ data showed a tighter (and therefore better) relationship between CI and Chl than between traditional band-ratios and Chl, which was further validated using global data collected concurrently by ship-borne and SeaWiFS satellite instruments. Model simulations showed that for low Chl waters, compared with the band-ratio algorithm, the CI-based algorithm (CIA) was more tolerant to changes in chlorophyll-specific backscattering coefficient, and performed similarly for different relative contributions of non-phytoplankton absorption. Simulations using existing atmospheric correction approaches further demonstrated that the CIA was much less sensitive than band-ratio algorithms to various errors induced by instrument noise and imperfect atmospheric correction (including sun glint and whitecap corrections). Image and time-series analyses of SeaWiFS and MODIS/Aqua data also showed improved performance in terms of reduced image noise, more coherent spatial and temporal patterns, and consistency between the two sensors. The reduction in noise and other errors is particularly useful to improve the detection of various ocean features such as eddies. Preliminary tests over MERIS and CZCS data indicate that the new approach should be generally applicable to all existing and future ocean color instruments.

Spatial extent and dissipation of the deep chlorophyll layer in Lake Ontario during the Lake Ontario lower foodweb assessment, 2003 and 2008

Science.gov (United States)

Watkins, J. M.; Weidel, Brian M.; Rudstam, L. G.; Holek, K. T.

2014-01-01

Increasing water clarity in Lake Ontario has led to a vertical redistribution of phytoplankton and an increased importance of the deep chlorophyll layer in overall primary productivity. We used in situ fluorometer profiles collected in lakewide surveys of Lake Ontario in 2008 to assess the spatial extent and intensity of the deep chlorophyll layer. In situ fluorometer data were corrected with extracted chlorophyll data using paired samples from Lake Ontario collected in August 2008. The deep chlorophyll layer was present offshore during the stratified conditions of late July 2008 with maximum values from 4-13 μg l-1 corrected chlorophyll a at 10 to 17 m depth within the metalimnion. Deep chlorophyll layer was closely associated with the base of the thermocline and a subsurface maximum of dissolved oxygen, indicating the feature's importance as a growth and productivity maximum. Crucial to the deep chlorophyll layer formation, the photic zone extended deeper than the surface mixed layer in mid-summer. The layer extended through most of the offshore in July 2008, but was not present in the easternmost transect that had a deeper surface mixed layer. By early September 2008, the lakewide deep chlorophyll layer had dissipated. A similar formation and dissipation was observed in the lakewide survey of Lake Ontario in 2003.

Comprehensive chlorophyll composition in the main edible seaweeds.

Science.gov (United States)

Chen, Kewei; Ríos, José Julián; Pérez-Gálvez, Antonio; Roca, María

2017-08-01

Natural chlorophylls present in seaweeds have been studied regarding their biological activities and health benefit effects. However, detailed studies regarding characterization of the complete chlorophyll profile either qualitatively and quantitatively are scarce. This work deals with the comprehensive spectrometric study of the chlorophyll derivatives present in the five main coloured edible seaweeds. The novel complete MS 2 characterization of five chlorophyll derivatives: chlorophyll c 2 , chlorophyll c 1 , purpurin-18 a, pheophytin d and phytyl-purpurin-18 a has allowed to obtain fragmentation patterns associated with their different structural features. New chlorophyll derivatives have been identified and quantified by first time in red, green and brown seaweeds, including some oxidative structures. Quantitative data of the chlorophyll content comes to achieve significant information for food composition databases in bioactive compounds. Copyright © 2017 Elsevier Ltd. All rights reserved.

Integrating Biology into the General Chemistry Laboratory: Fluorometric Analysis of Chlorophyll "a"

Science.gov (United States)

Wesolowski, Meredith C.

2014-01-01

A laboratory experiment that introduces fluorometry of chlorophyll "a" at the general chemistry level is described. The use of thin-layer chromatography to isolate chlorophyll "a" from spirulina and leaf matter enables quantification of small amounts of chlorophyll "a" via fluorometry. Student results were reasonably…

Water deficit and salt stress diagnosis through LED induced chlorophyll fluorescence analysis in Jatropha curcas L. oil plants for biodiesel

Science.gov (United States)

Gouveia-Neto, Artur S.; Silva, Elias A., Jr.; Oliveira, Ronaldo A.; Cunha, Patrícia C.; Costa, Ernande B.; Câmara, Terezinha J. R.; Willadino, Lilia G.

2011-02-01

Light-emitting-diode induced chlorophyll fluorescence analysis is employed to investigate the effect of water and salt stress upon the growth process of physicnut(jatropha curcas) grain oil plants for biofuel. Red(Fr) and far-red (FFr) chlorophyll fluorescence emission signals around 685 nm and 735 nm, respectively, were observed and examined as a function of the stress intensity(salt concentration and water deficit) for a period of time of 30 days. The chlorophyll fluorescence(ChlF) ratio Fr/FFr which is a valuable nondestructive and nonintrusive indicator of the chlorophyll content of leaves was exploited to monitor the level of stress experienced by the jatropha plants. The ChlF technique data indicated that salinity plays a minor role in the chlorophyll concentration of leaves tissues for NaCl concentrations in the 25 to 200 mM range, and results agreed quite well with those obtained using conventional destructive spectrophotometric methods. Nevertheless, for higher NaCl concentrations a noticeable decrease in the Chl content was observed. The Chl fluorescence ratio analysis also permitted detection of damage caused by water deficit in the early stages of the plants growing process. A significant variation of the Fr/FFr ratio was observed sample in the first 10 days of the experiment when one compared control and nonwatered samples. The results suggest that the technique may potentially be applied as an early-warning indicator of stress caused by water deficit.

Genetic analysis of sunflower chlorophyll mutants

International Nuclear Information System (INIS)

Mashkina, E.V.; Guskov, E.P.

2001-01-01

The method of getting the chlorophyll mutations in sunflower was developed by Y.D. Beletskii in 1969 with the use of N-nitroso-N-methylurea (NMH). Certain concentrations of NMH are known to induce plastid mutations in growing seeds, and their yield depends on the duration of the exposure. The given work presented studies on the influence of rifampicin (R) and 2,4-dinitrophenol (DNP) on the genetic activity NMH, as an inductor of plastid and nuclear mutations

Parameterization of the chlorophyll a-specific in vivo light absorption coefficient covering estuarine, coastal and oceanic waters

DEFF Research Database (Denmark)

Stæhr, P. A.; Markager, S.

2004-01-01

We evaluated models predicting the spectral chlorophyll-a (Chl a)-specific absorption coefficient (a*ph (¿)) from Chl a concentration [Chl a] on the basis of 465 phytoplankton absorption spectra collected in estuarine, coastal and oceanic waters. A power model on ln-transformed data provided...

HYDROPT: A fast and flexible method to retrieve chlorophyll-a from multispectral satellite observations of optically complex coastal waters

NARCIS (Netherlands)

van der Woerd, H.J.; Pasterkamp, R.

2008-01-01

We present a generic innovative algorithm for remote sensing of coastal waters that can deal with a large range of concentrations of chlorophyll-a, SPM and CDOM and their inherent optical properties. The algorithm is based on the exact solutions of the HYDROLIGHT numerical radiative transfer model

Gas exchanges in peach palms as a function of the spad chlorophyll meter readings

Directory of Open Access Journals (Sweden)

Maria Luiza Sant'anna Tucci

2011-10-01

Full Text Available The close relationship between the chlorophyll-meters readings and the total chlorophyll and nitrogen contents in leaves, has allowed their evaluation both in annual and perennial species. Besides, some physiological events such as the CO2 assimilation have also been estimated by chlorophyll meters. This work was carried out aiming to evaluate the gas exchanges of peach palms as a function of the chlorophyll SPAD-Meter readings. Three year-old peach palms from Yurimaguas, Peru were studied in Ubatuba, SP, Brazil, spaced 2 x 1 m in area under a natural gradient of organic matter which allowed four plots to be considered, according to the peach palms leaves colors, from light yellow to dark green. The SPAD readings and the stomatal frequency of leaflets were evaluated. The photosynthetic photon flux density (PPFD, μmol m-2 s-1, the leaf temperature (Tleaf, ºC, the CO2 assimilation (A, μmol m-2 s-1, the stomatal conductance (g s, mol m-2 s-1, the transpiration (E, mmol m-2 s-1 and the intercellular CO2 concentration (Ci, μmol mol-1 were evaluated with a portable infrared gas analyzer (LCA-4, ADC BioScientific Ltd., Great Amwell, U.K.. A linear increase in the CO2 assimilation as a function of the SPAD readings (y = -0.34 + 0.19x, R² = 0.99, indicates that they can be a rapid and cheap complementary method to evaluate in peach palms some important physiological events, such as CO2 assimilation.

The dynamics of suspended particulate matter (SPM) and chlorophyll-a from intratidal to annual time scales in a coastal turbidity maximum

Science.gov (United States)

van der Hout, C. M.; Witbaard, R.; Bergman, M. J. N.; Duineveld, G. C. A.; Rozemeijer, M. J. C.; Gerkema, T.

2017-09-01

The analysis of 1.8 years of data gives an understanding of the response to varying forcing of suspended particulate matter (SPM) and chlorophyll-a (CHL-a) in a coastal turbidity maximum zone (TMZ). Both temporal and vertical concentration variations in the near-bed layer (0-2 m) in the shallow (11 m deep) coastal zone at 1 km off the Dutch coast are shown. Temporal variations in the concentration of both parameters are found on tidal and seasonal scales, and a marked response to episodic events (e.g. storms). The seasonal cycle in the near-bed CHL-a concentration is determined by the spring bloom. The role of the wave climate as the primary forcing in the SPM seasonal cycle is discussed. The tidal current provides a background signal, generated predominantly by local resuspension and settling and a minor role is for advection in the cross-shore and the alongshore direction. We tested the logarithmic Rouse profile to the vertical profiles of both the SPM and the CHL-a data, with respectively 84% and only 2% success. The resulting large percentage of low Rouse numbers for the SPM profiles suggest a mixed suspension is dominant in the TMZ, i.e. surface SPM concentrations are in the same order of magnitude as near-bed concentrations.

Characterizing the production and retention of dissolved iron as Fe(II) across a natural gradient in chlorophyll concentrations in the Southern Drake Passage - Final Technical Report

Energy Technology Data Exchange (ETDEWEB)

Katherine Barbeau

2007-04-10

Recent mesoscale iron fertilization studies in the Southern Ocean (e.g. SOIREE, EisenEx, SOFeX) have demonstrated the importance of iron as a limiting factor for phytoplankton growth in these high nutrient, low-chlorophyll (HNLC) waters. Results of these experiments have demonstrated that factors which influence the biological availability of the iron supplied to phytoplankton are crucial in bloom development, longevity, and generation of carbon export flux. These findings have important implications for the future development of iron fertilization protocols to enhance carbon sequestration in high-latitude oceans. In particular, processes which lead to the mobilization and retention of iron in dissolved form in the upper ocean are important in promoting continued biological availability of iron. Such processes can include photochemical redox cycling, which leads to the formation of soluble reduced iron, Fe(II), within iron-enriched waters. Creation of effective fertilization schemes will thus require more information about Fe(II) photoproduction in Southern Ocean waters as a means to retain new iron within the euphotic zone. To contribute to our knowledge base in this area, this project was funded by DOE with a goal of characterizing the production and retention of dissolved Fe as Fe(II) in an area of the southern Drake Passage near the Shackleton Transverse Ridge, a region with a strong recurrent chlorophyll gradient which is believed to be a site of natural iron enrichment in the Southern Ocean. This area was the focus of a multidisciplinary NSF/OPP-funded investigation in February 2004 (OPP02-30443, lead PI Greg Mitchell, SIO/UCSD) to determine the influence of mesoscale circulation and iron transport with regard to the observed patterns in sea surface chlorophyll in the region near the Shackleton Transverse Ridge. A number of parameters were assessed across this gradient in order to reveal interactions between plankton community structure and iron distributions

A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock

Directory of Open Access Journals (Sweden)

Tao Li

2016-09-01

Full Text Available Microalgae oil is an optimal feedstock for nutraceutical, pharmaceutical and biodiesel production, but its high levels of chlorophyll limit its large-scale application. To date, few effective approaches have been developed to remove chlorophyll from microalgae oil. The main purpose of this study was to present a preprocessing method of algae oil feedstock (Scenedesmus to remove chlorophyll by saponification. The results showed that 96% of chlorophyll in biomass was removed. High quality orange transparent oil could be extracted from the chlorophyll reduced biomass. Specifically, the proportion of neutral lipids and saturation levels of fatty acids increased, and the pigments composition became carotenoids-based. The critical parameters of chlorophyll reduced biodiesel conformed to the standards of the USA, China and EU. Sodium copper chlorophyllin could be prepared from the bleaching effluent. The results presented herein offer a useful pathway to improve the quality of microalgae oil and reduce the cost of microalgae biodiesel.

A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock

Science.gov (United States)

Li, Tao; Xu, Jin; Wu, Hualian; Wang, Guanghua; Dai, Shikun; Fan, Jiewei; He, Hui; Xiang, Wenzhou

2016-01-01

Microalgae oil is an optimal feedstock for nutraceutical, pharmaceutical and biodiesel production, but its high levels of chlorophyll limit its large-scale application. To date, few effective approaches have been developed to remove chlorophyll from microalgae oil. The main purpose of this study was to present a preprocessing method of algae oil feedstock (Scenedesmus) to remove chlorophyll by saponification. The results showed that 96% of chlorophyll in biomass was removed. High quality orange transparent oil could be extracted from the chlorophyll reduced biomass. Specifically, the proportion of neutral lipids and saturation levels of fatty acids increased, and the pigments composition became carotenoids-based. The critical parameters of chlorophyll reduced biodiesel conformed to the standards of the USA, China and EU. Sodium copper chlorophyllin could be prepared from the bleaching effluent. The results presented herein offer a useful pathway to improve the quality of microalgae oil and reduce the cost of microalgae biodiesel. PMID:27618070

A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock.

Science.gov (United States)

Li, Tao; Xu, Jin; Wu, Hualian; Wang, Guanghua; Dai, Shikun; Fan, Jiewei; He, Hui; Xiang, Wenzhou

2016-09-07

Microalgae oil is an optimal feedstock for nutraceutical, pharmaceutical and biodiesel production, but its high levels of chlorophyll limit its large-scale application. To date, few effective approaches have been developed to remove chlorophyll from microalgae oil. The main purpose of this study was to present a preprocessing method of algae oil feedstock (Scenedesmus) to remove chlorophyll by saponification. The results showed that 96% of chlorophyll in biomass was removed. High quality orange transparent oil could be extracted from the chlorophyll reduced biomass. Specifically, the proportion of neutral lipids and saturation levels of fatty acids increased, and the pigments composition became carotenoids-based. The critical parameters of chlorophyll reduced biodiesel conformed to the standards of the USA, China and EU. Sodium copper chlorophyllin could be prepared from the bleaching effluent. The results presented herein offer a useful pathway to improve the quality of microalgae oil and reduce the cost of microalgae biodiesel.

A model for chlorophyll fluorescence and photosynthesis at leaf scale

NARCIS (Netherlands)

Tol, van der C.; Verhoef, W.; Rosema, A.

2009-01-01

This paper presents a leaf biochemical model for steady-state chlorophyll fluorescence and photosynthesis of C3 and C4 vegetation. The model is a tool to study the relationship between passively measured steady-state chlorophyll fluorescence and actual photosynthesis, and its evolution during the

Assessment of chlorophyll variability along the northwestern coast of Iberian Peninsula

Science.gov (United States)

Picado, A.; Alvarez, I.; Vaz, N.; Varela, R.; Gomez-Gesteira, M.; Dias, J. M.

2014-10-01

The northwestern coast of the Iberian Peninsula is characterized by a high primary production mainly supported by coastal upwelling, creating an extraordinary commercial interest for fisheries and aquaculture. Considering chlorophyll-a (Chl-a) as an indicator of primary production, its spatio-temporal variability was researched in this study in the surface water of this upwelling region from 1998 to 2007. Satellite derived Chl-a, Sea Surface Temperature (SST) and Ekman transport data as well as the inflow of the main rivers discharging into the study area were used to investigate the origin of the Chl-a concentration. Empirical Orthogonal Function (EOF) analysis of weekly Chl-a images was performed, as well as correlation analysis between Chl-a concentration, Ekman transport and river discharge. EOF results suggest that the highest Chl-a concentration occurs near the coast up to 60 km offshore. The interannual variability of Chl-a, SST and Ekman transport was also studied considering summer and winter months. Generally, 2005, 2006 and 2007 were the most productive years during the summer months with high Chl-a concentrations along the coast associated to the strong upwelling conditions observed. Otherwise, 1998 seemed to be the most productive year during winter. The absence of upwelling favorable conditions together with localized low SST and considerable discharges, suggests that the high Chl-a concentrations observed during this period are mainly due to the entrance of nutrients through river runoff. However, in winter, high concentrations of colored dissolved organic matter (CDOM), associated with river runoff, are present in the ocean surface, leading to an erroneous strong signal of the satellite. During winter correlations of 0.58 and 0.49 were found between Chl-a concentration and Douro and Minho discharges, respectively, evidencing that high Chl-a concentration was related with river runoff. Otherwise, during summer, Chl-a and Ekman transport exhibited a

Chlorophyll modulation of sea surface temperature in the Arabian Sea in a mixed-layer isopycnal general circulation model

Digital Repository Service at National Institute of Oceanography (India)

Nakamoto, S.; PrasannaKumar, S.; Muneyama, K.; Frouin, R.

, embedded in the ocean isopycnal general circulation model (OPYC). A higher abundance of chlorophyll in October than in April in the Arabian Sea increases absorption of solar irradiance and heating rate in the upper ocean, resulting in decreasing the mixed...

Chlorophyll as a measure of plant health: Agroecological aspects

Directory of Open Access Journals (Sweden)

Danijela Pavlović

2014-03-01

Full Text Available As photosynthesis is the basic process during which light energy is absorbed and converted into organic matter, the importance of the plant pigment chlorophyll (a and b forms as an intermediary in transformation of the absorbed solar energy and its activity in the process of photosynthesis and synthesis of organic substances in plants are crucial. Therefore, this paper provides an overview of methods for monitoring the optical activity of chlorophyll molecules and methods (non-destructive and destructive for quantification of chlorophyll in plants. These methods are used to estimate the effects of different stress factors (abiotic, biotic and xenobiotic on the efficiency of photosynthesis and bioproductivity, aiming to assess the impact that these limiting factors have on the yield of various cultivars. Also, those methods for analysis of chlorophyll optical activity and/or content are appropriate for assessing the reaction of weed species to different agricultural practices (mineral nutrition, treatment by herbicides, etc. and studies of different aspects of weed ecophysiology and their influence on crop harvest.

Does Primary Productivity Turn Up the Volume? Exploring the Relationship Between Chlorophyll a and the Soundscape of Coral Reefs in the Pacific.

Science.gov (United States)

Fisher-Pool, Pollyanna I; Lammers, Marc O; Gove, Jamison; Wong, Kevin B

2016-01-01

Chlorophyll is the basis for ecosystem productivity in most marine environments. We report on an ongoing effort to examine whether ambient sounds are tied to chlorophyll levels. We hypothesized that an increase in food-web available energy will be distributed across trophic levels, eventually reaching sound-producing animals and increasing acoustic levels. To test our hypothesis, we compared reef environments to explore links between soundscapes and chlorophyll a concentrations. The study sites resided in disparate oceanographic regimes that experienced substantially different oceanographic conditions. We anticipated that the results would show differing patterns of primary productivity between sites and therefore would be reflected in the soundscapes.

SHORT COMMUNICATION ISOLATION OF CHLOROPHYLL A ...

African Journals Online (AJOL)

a

chromatography (CCC) technique has been applied to the separation of chlorophyll a from ... auxiliary gas flow rate, 0.06 L min-1; ion spray voltage, 3.5 kV; capillary .... This work presents a successful application of column chromatography ...

Identification of Genes Associated with Chlorophyll Accumulation in Flower Petals

Science.gov (United States)

Ohmiya, Akemi; Hirashima, Masumi; Yagi, Masafumi; Tanase, Koji; Yamamizo, Chihiro

2014-01-01

Plants have an ability to prevent chlorophyll accumulation, which would mask the bright flower color, in their petals. In contrast, leaves contain substantial amounts of chlorophyll, as it is essential for photosynthesis. The mechanisms of organ-specific chlorophyll accumulation are unknown. To identify factors that determine the chlorophyll content in petals, we compared the expression of genes related to chlorophyll metabolism in different stages of non-green (red and white) petals (very low chlorophyll content), pale-green petals (low chlorophyll content), and leaves (high chlorophyll content) of carnation (Dianthus caryophyllus L.). The expression of many genes encoding chlorophyll biosynthesis enzymes, in particular Mg-chelatase, was lower in non-green petals than in leaves. Non-green petals also showed higher expression of genes involved in chlorophyll degradation, including STAY-GREEN gene and pheophytinase. These data suggest that the absence of chlorophylls in carnation petals may be caused by the low rate of chlorophyll biosynthesis and high rate of degradation. Similar results were obtained by the analysis of Arabidopsis microarray data. In carnation, most genes related to chlorophyll biosynthesis were expressed at similar levels in pale-green petals and leaves, whereas the expression of chlorophyll catabolic genes was higher in pale-green petals than in leaves. Therefore, we hypothesize that the difference in chlorophyll content between non-green and pale-green petals is due to different levels of chlorophyll biosynthesis. Our study provides a basis for future molecular and genetic studies on organ-specific chlorophyll accumulation. PMID:25470367

Changes in chlorophyll and polyphenols content in Camellia sinensis var. sinensis at different stage of leaf maturity

Science.gov (United States)

Prawira-Atmaja, M. I.; Shabri; Khomaini, H. S.; Maulana, H.; Harianto, S.; Rohdiana, D.

2018-03-01

Chlorophyll and polyphenols are chemical compound related to parameter quality of green tea. We studied the variation of chlorophyll and polyphenol in the development stage of tea leaves (bud, 1st, 2nd, 3rd, and 4th). Five clones of tea (Camelia sinensis var. sinensis) from Indonesia and a clone from Japan were used in this study. The results showed that total chlorophyll and total polyphenol content in bud between 1.59-2.15 mg/g (db) and 12.24-14.59% respectively. The concentration of chlorophyll increased significantly with developments stage of leaf while total polyphenol tended to decrease with leaf maturity. Pearson Correlation analysis showed that chlorophyll content was negatively correlated (r = -0.83; p = 0.05) with total polyphenol during developmental stage of tea leaves. Results suggests that five clones of tea from Indonesia have similar quality with tea clone from Japan in chlorophyll and polyphenol content. The present study also provides guidelines on application plucking standard to produce high quality of green tea.

Estimation of surface area concentration of workplace incidental nanoparticles based on number and mass concentrations

Science.gov (United States)

Park, J. Y.; Ramachandran, G.; Raynor, P. C.; Kim, S. W.

2011-10-01

Surface area was estimated by three different methods using number and/or mass concentrations obtained from either two or three instruments that are commonly used in the field. The estimated surface area concentrations were compared with reference surface area concentrations (SAREF) calculated from the particle size distributions obtained from a scanning mobility particle sizer and an optical particle counter (OPC). The first estimation method (SAPSD) used particle size distribution measured by a condensation particle counter (CPC) and an OPC. The second method (SAINV1) used an inversion routine based on PM1.0, PM2.5, and number concentrations to reconstruct assumed lognormal size distributions by minimizing the difference between measurements and calculated values. The third method (SAINV2) utilized a simpler inversion method that used PM1.0 and number concentrations to construct a lognormal size distribution with an assumed value of geometric standard deviation. All estimated surface area concentrations were calculated from the reconstructed size distributions. These methods were evaluated using particle measurements obtained in a restaurant, an aluminum die-casting factory, and a diesel engine laboratory. SAPSD was 0.7-1.8 times higher and SAINV1 and SAINV2 were 2.2-8 times higher than SAREF in the restaurant and diesel engine laboratory. In the die casting facility, all estimated surface area concentrations were lower than SAREF. However, the estimated surface area concentration using all three methods had qualitatively similar exposure trends and rankings to those using SAREF within a workplace. This study suggests that surface area concentration estimation based on particle size distribution (SAPSD) is a more accurate and convenient method to estimate surface area concentrations than estimation methods using inversion routines and may be feasible to use for classifying exposure groups and identifying exposure trends.

Estimation of surface area concentration of workplace incidental nanoparticles based on number and mass concentrations

International Nuclear Information System (INIS)

Park, J. Y.; Ramachandran, G.; Raynor, P. C.; Kim, S. W.

2011-01-01

Surface area was estimated by three different methods using number and/or mass concentrations obtained from either two or three instruments that are commonly used in the field. The estimated surface area concentrations were compared with reference surface area concentrations (SA REF ) calculated from the particle size distributions obtained from a scanning mobility particle sizer and an optical particle counter (OPC). The first estimation method (SA PSD ) used particle size distribution measured by a condensation particle counter (CPC) and an OPC. The second method (SA INV1 ) used an inversion routine based on PM1.0, PM2.5, and number concentrations to reconstruct assumed lognormal size distributions by minimizing the difference between measurements and calculated values. The third method (SA INV2 ) utilized a simpler inversion method that used PM1.0 and number concentrations to construct a lognormal size distribution with an assumed value of geometric standard deviation. All estimated surface area concentrations were calculated from the reconstructed size distributions. These methods were evaluated using particle measurements obtained in a restaurant, an aluminum die-casting factory, and a diesel engine laboratory. SA PSD was 0.7–1.8 times higher and SA INV1 and SA INV2 were 2.2–8 times higher than SA REF in the restaurant and diesel engine laboratory. In the die casting facility, all estimated surface area concentrations were lower than SA REF . However, the estimated surface area concentration using all three methods had qualitatively similar exposure trends and rankings to those using SA REF within a workplace. This study suggests that surface area concentration estimation based on particle size distribution (SA PSD ) is a more accurate and convenient method to estimate surface area concentrations than estimation methods using inversion routines and may be feasible to use for classifying exposure groups and identifying exposure trends.

Chlorophyll Degradation: The Tocopherol Biosynthesis-Related Phytol Hydrolase in Arabidopsis Seeds Is Still Missing1[C][W][OPEN

Science.gov (United States)

Zhang, Wei; Liu, Tianqi; Ren, Guodong; Hörtensteiner, Stefan; Zhou, Yongming; Cahoon, Edgar B.; Zhang, Chunyu

2014-01-01

Phytyl diphosphate (PDP) is the prenyl precursor for tocopherol biosynthesis. Based on recent genetic evidence, PDP is supplied to the tocopherol biosynthetic pathway primarily by chlorophyll degradation and sequential phytol phosphorylation. Three enzymes of Arabidopsis (Arabidopsis thaliana) are known to be capable of removing the phytol chain from chlorophyll in vitro: chlorophyllase1 (CLH1), CLH2, and pheophytin pheophorbide hydrolase (PPH), which specifically hydrolyzes pheophytin. While PPH, but not chlorophyllases, is required for in vivo chlorophyll breakdown during Arabidopsis leaf senescence, little is known about the involvement of these phytol-releasing enzymes in tocopherol biosynthesis. To explore the origin of PDP for tocopherol synthesis, seed tocopherol concentrations were determined in Arabidopsis lines engineered for seed-specific overexpression of PPH and in single and multiple mutants in the three genes encoding known dephytylating enzymes. Except for modestly increasing tocopherol content observed in the PPH overexpressor, none of the remaining lines exhibited significantly reduced tocopherol concentrations, suggesting that the known chlorophyll-derived phytol-releasing enzymes do not play major roles in tocopherol biosynthesis. Tocopherol content of seeds from double mutants in NONYELLOWING1 (NYE1) and NYE2, regulators of chlorophyll degradation, had modest reduction compared with wild-type seeds, although mature seeds of the double mutant retained significantly higher chlorophyll levels. These findings suggest that NYEs may play limited roles in regulating an unknown tocopherol biosynthesis-related phytol hydrolase. Meanwhile, seeds of wild-type over-expressing NYE1 had lower tocopherol levels, suggesting that phytol derived from NYE1-dependent chlorophyll degradation probably doesn’t enter tocopherol biosynthesis. Potential routes of chlorophyll degradation are discussed in relation to tocopherol biosynthesis. PMID:25059706

Effects of sodium pentaborate pentahydrate exposure on Chlorella vulgaris growth, chlorophyll content, and enzyme activities.

Science.gov (United States)

Chen, Xueqing; Pei, Yuansheng

2016-10-01

Sodium pentaborate pentahydrate (SPP) is a rare mineral. In this study, SPP was synthesized from boric acid and borax through low-temperature crystallization, and its effects on the growth of the alga, Chlorella vulgaris (C. vulgaris) were assessed. The newly synthesized SPP was characterized by chemical analysis, X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis, and differential thermal analysis. The changes in C. vulgaris growth, chlorophyll content, and enzyme activities upon exposure to SPP for 168h were evaluated. Results showed that SPP treatment was detrimental to C. vulgaris growth during the first 24-120h of exposure. The harmful effects, however, diminished over time (168h), even at an effective medium concentration of 226.37mg BL(-1) (the concentration of boron applied per liter of culture medium). A similar trend was observed for chlorophyll content (chlorophyll a and b) and indicated that the photosynthesis of C. vulgaris was not affected and that high levels of SPP may even promote chlorophyll synthesis. Superoxide dismutase and catalase activities of C. vulgaris increased during 24-120h exposure to SPP, but these activities gradually decreased as culture time progressed. In other words, the initial detrimental effects of synthetic SPP on C. vulgaris were temporary and reversible. This research provides a scientific basis for applications of SPP in the environment. Copyright © 2016 Elsevier Inc. All rights reserved.

Presence of a chlorophyll d-like pigment in Chlorella extracts

NARCIS (Netherlands)

Michel-Wolwertz, M.R.; Sironval, C.; Goedheer, J.C.

1965-01-01

Three chlorophyll a isomers (a₁, a₂ and a₃) were separated by the chromatography of Chlorella extracts on paper 1. One of these, chlorophyll (a₃) showed additional absorption bands at 688 and 455 mμ in diethyl ether. Chromatographic analysis could not decide whether these bands were due to a₃ or

Trends in Pinus ponderosa foliar pigment concentration due to chronic exposure of ozone and acid rain

International Nuclear Information System (INIS)

Neuman, L.; Houpis, J.; Anderson, P.

1991-01-01

To determine the effects of ozone and acid rain on mature Ponderosa pine trees, Lawrence Livermore National Lab. has collaborated with University of California Berkeley, University of California Davis, California State University Chico, and the US Forest Service at the latter's Chico Tree Improvement Center. Foliar tissue from mature grafted scions of Pinus ponderosa were exposed to two times ambient ozone for ten months and to acid rain (3.0 pH) weekly for 10 weeks using branch exposure chambers. Pigment extracts were analyzed spectrophotometrically for concentrations of chlorophylls a and b, and carotenoid pigments, at 662 nm, 644 nm, and 470 nm, respectively. Pigment concentrations were expressed on a surface area basis. Preliminary results revealed that chlorophyll a showed a downward trend due to the ozone treatment. Acid rain caused no effects on these three pigments, however, chlorophyll b showed an upward trend due to the interaction of ozone and acid rain. The carotenoid pigments showed no changes due to the treatments either singly, or in combination

Chlorophyll loss associated with heat-induced senescence in bentgrass.

Science.gov (United States)

Jespersen, David; Zhang, Jing; Huang, Bingru

2016-08-01

Heat stress-induced leaf senescence is characterized by the loss of chlorophyll from leaf tissues. The objectives of this study were to examine genetic variations in the level of heat-induced leaf senescence in hybrids of colonial (Agrostis capillaris)×creeping bentgrass (Agrostis stolonifera) contrasting in heat tolerance, and determine whether loss of leaf chlorophyll during heat-induced leaf senescence was due to suppressed chlorophyll synthesis and/or accelerated chlorophyll degradation in the cool-season perennial grass species. Plants of two hybrid backcross genotypes ('ColxCB169' and 'ColxCB190') were exposed to heat stress (38/33°C, day/night) for 28 d in growth chambers. The analysis of turf quality, membrane stability, photochemical efficiency, and chlorophyll content demonstrated significant variations in the level of leaf senescence induced by heat stress between the two genotypes, with ColXCB169 exhibiting a lesser degree of decline in chlorophyll content, photochemical efficiency and membrane stability than ColXCB190. The assays of enzymatic activity or gene expression of several major chlorophyll-synthesizing (porphobilinogen deaminase, Mg-chelatase, protochlorophyllide-reductase) and chlorophyll-degrading enzymes (chlorophyllase, pheophytinase, and chlorophyll-degrading peroxidase) indicated heat-induced decline in leaf chlorophyll content was mainly due to accelerated chlorophyll degradation, as manifested by increased gene expression levels of chlorophyllase and pheophytinase, and the activity of pheophytinase (PPH), while chlorophyll-synthesizing genes and enzymatic activities were not differentially altered by heat stress in the two genotypes. The analysis of heat-induced leaf senescence of pph mutants of Arabidopsis further confirmed that PPH could be one enzymes that plays key roles in regulating heat-accelerated chlorophyll degradation. Further research on enzymes responsible in part for the loss of chlorophyll during heat

Dustfall Effect on Hyperspectral Inversion of Chlorophyll Content - a Laboratory Experiment

Science.gov (United States)

Chen, Yuteng; Ma, Baodong; Li, Xuexin; Zhang, Song; Wu, Lixin

2018-04-01

Dust pollution is serious in many areas of China. It is of great significance to estimate chlorophyll content of vegetation accurately by hyperspectral remote sensing for assessing the vegetation growth status and monitoring the ecological environment in dusty areas. By using selected vegetation indices including Medium Resolution Imaging Spectrometer Terrestrial Chlorophyll Index (MTCI) Double Difference Index (DD) and Red Edge Position Index (REP), chlorophyll inversion models were built to study the accuracy of hyperspectral inversion of chlorophyll content based on a laboratory experiment. The results show that: (1) REP exponential model has the most stable accuracy for inversion of chlorophyll content in dusty environment. When dustfall amount is less than 80 g/m2, the inversion accuracy based on REP is stable with the variation of dustfall amount. When dustfall amount is greater than 80 g/m2, the inversion accuracy is slightly fluctuation. (2) Inversion accuracy of DD is worst among three models. (3) MTCI logarithm model has high inversion accuracy when dustfall amount is less than 80 g/m2; When dustfall amount is greater than 80 g/m2, inversion accuracy decreases regularly and inversion accuracy of modified MTCI (mMTCI) increases significantly. The results provide experimental basis and theoretical reference for hyperspectral remote sensing inversion of chlorophyll content.

Fluorescência e teores de clorofilas em abacaxizeiro cv. pérola submetido a diferentes concentrações de sulfato de amônio Fluorescence and levels of chlorophyll in pineapple plants cv. perola submitted to different concentration of ammonium sulphate

Directory of Open Access Journals (Sweden)

Darlene Ana de Paula Vieira

2010-06-01

Full Text Available O presente trabalho teve como objetivo a análise da emissão da fluorescência da clorofila a e dos teores de clorofilas em plantas de Ananas comosus (L. Merril cv pérola, cultivadas em casa de vegetação, submetidas a quatro concentrações de nitrogênio por adição ou não de sulfato de amônio, de acordo com os seguintes tratamentos: Tº= 0 T1/2 = 15; T1 = 30; e T2 = 60 mg/kg solo. As determinações de fluorescência mínima (F0, máxima (Fm, variável (Fv, terminal (Ft e da eficiência fotoquímica máxima (Fv/Fm de folhas adaptadas ao escuro foram realizadas ao longo do dia, aos cinco dias após a segunda aplicação de sulfato de amônio, efetuada 120 dias após o transplantio. A adição de sulfato de amônio afetou a fluorescência variável e a máxima, mas não afetou a fluorescência mínima, a terminal nem a eficiência fotoquímica. Houve diferenças significativas entre os valores das variáveis da fluorescência ao longo do dia em que foram feitas as leituras. Houve diferenças nos teores de clorofilas foliares, em função das concentrações de sulfato de amônio aplicadas, com aumento para clorofila a e para a relação clorofila a/b, mas não para clorofila b.The present research aimed to analyze chlorophyll a fluorescence emission as well as chlorophyll levels in Ananas comosus (L. Merril cv Pérola grown under greenhouse conditions and submitted to four concentration of nitrogen, through addition or not of ammonium sulphate according to the following treatments: Tº= 0.000; T1/2 = 0.015; T1 =0.030; and T2 = 0.060 g/kg soil. Determinations of minimum (F0, maximum (Fm, variable (Fv, and terminal (Ft fluorescence and maximum photochemical efficiency (Fv/Fm of dark-adapted leaves were carried out during the day, five days after the second application of ammonium sulphate, carried out 120 days after the transplant. The results showed that the addition of ammonium sulphate affected variable and maximum fluorescence, but not

LAI and chlorophyll estimation for a heterogeneous grassland using hyperspectral measurements

NARCIS (Netherlands)

Darvishzadeh, R.; Skidmore, A.K.; Schlerf, M.; Atzberger, C.; Corsi, F.; Cho, M.A.

2008-01-01

The study shows that leaf area index (LAI), leaf chlorophyll content (LCC) and canopy chlorophyll content (CCC) can be mapped in a heterogeneous Mediterranean grassland from canopy spectral reflectance measurements. Canopy spectral measurements were made in the field using a GER 3700

A study of the low-lying singlet and triplet electronic states of chlorophyll A and B

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Etinski Mihajlo

2013-01-01

Full Text Available Chlorophylls have been extensively investigated both experimentally and theoretically owing to the fact that they are essential for photosynthesis. We have studied two forms of chlorophyll, chlorophyll a and chlorophyll b, by means of density functional theory. Optimization of S0, S1 and T1 states was performed with the B3-LYP functional. The computed fluorescence lifetimes show good agreement with the available experimental data. The electronic adiabatic energies of S1 and T1 states are 2.09/2.12 and 1.19/1.29 eV for chlorophyll a and chlorophyll b respectively. We discussed the implications of this results on the triplet formation. Also, the calculated vertical ionization potentials shows good agreement with the experimental results. [Projekat Ministarstva nauke Reoublike Srbije, br. 172040

Spectrum and frequency of chlorophyll mutations in urdbean (Vigna mungo L. Hepper) induced by EMS and gamma rays

International Nuclear Information System (INIS)

Sharma, A.K.; Singh, V.P.; Sarma, M.K.

2006-01-01

In mutation breeding experiment, plants with altered characteristics such as chlorophyll changes, sterility, plant lethality etc. could be the marker of the mutability of a variety. In fact, spectrum and frequency of chlorophyll mutations have been studied in the great detail. The chlorophyll mutation is the clear-cut indication of non-directional nature of mutation and possibility of induction of useful mutations. The spectrum and frequency of chlorophyll mutation was estimated by using gamma rays (100, 200, 300 and 400 Gy doses), EMS (0.2, 0.4, 0.6 and 0.8%) and combination of gamma rays (100, 200, 300 400 Gy) with 0.2 % concentration EMS on two cultivars, namely, Pant Urd-19 and Pant Urd-30 of urdbean ( Vigna mungo L. Hepper). Five different types of chlorophyll mutations viz., albina, xantha, viridis, chlorina and maculata were identified in both the cultivars. Almost all the combination treatments produced maximum frequency and wider spectrum of chlorophyll mutations followed by single treatment of gamma rays or EMS. The frequency of chlorophyll mutation increased with higher doses of mutagens but decreased at highest dose. Proc. Nat. Acad. Sci. India. 76(8), I, 2006. 64-68. (author)

Investigations on gamma ray induced chlorophyll variegated mutants

International Nuclear Information System (INIS)

Datta, S.K.; Dwivedi, A.K.; Banerji, B.K.

1995-01-01

Considering economic importance of chlorophyll variegation in floriculture trade an attempt was made for cytological, anatomical and biochemical analysis of four Bougainvillea and Lantana depressa chlorophyll variegated mutants for better and clear understanding of origin of chlorophyll variegation. No cytological evidence could be detected for their origin. Anatomical and biochemical examinations revealed that chlorophyll variegation in these mutants were due to changes in biosynthesis pathways and time of chlorophyll synthesis in palisade and spongy mesophyll cells. (author). 7 refs., 3 figs., 3 tabs

Gas exchange, dry weights, and chlorophyll contents of Populus tremuloides seedlings grown from gamma-irradiated seeds

International Nuclear Information System (INIS)

Salmonson, B.J.; Zavitkovski, J.

1977-01-01

Photosynthesis and respiration rates of Populus tremuloides seedlings grown from seeds acutely irradiated with gamma rays (at levels of 0, 0.47, 0.94, 1.8, 3.7, 7.5, and 15 kr) were measured using a closed system and infrared (IR) gas analyzer. Dry weights of seedling roots and shoots and chlorophyll contents were also determined. In general, gamma irradiation of seed had little effect on subsequent gas-exchange processes in the plant. Net photosynthesis and dark respiration rates of the seedling at any radiation level were not significantly different from those of the control group. Pooled net photosynthesis of all irradiated seedlings was nonsignificantly lower than that of the control seedlings. The data suggested a slight stimulation in dry-matter production of shoots by low levels of gamma radiation. At the highest levels of radiation, shoot dry weight was significantly reduced. Root production displayed a pattern similar to that of shoots. No trends different from controls were evident in the shoot/root ratios. Total chlorophyll concentrations of the seedling increased over the controls by radiation treatment. Age and radiation effects were evident in the chlorophyll a/b ratios. The chlorophyll a/b ratios decreased with age in both the control and treatment groups. Although in seedlings 39 through 46 days old chlorophyll a/b ratios were higher in the control, chlorophyll a/b ratios were significantly higher in the treatment seedlings at 68 days. Changes in chlorophyll a were primarily responsible for this change

Differential effects of glyphosate and aminomethylphosphonic acid (AMPA) on photosynthesis and chlorophyll metabolism in willow plants.

Science.gov (United States)

Gomes, Marcelo Pedrosa; Le Manac'h, Sarah Gingras; Maccario, Sophie; Labrecque, Michel; Lucotte, Marc; Juneau, Philippe

2016-06-01

We used a willow species (Salix miyabeana cultivar SX64) to examine the differential secondary-effects of glyphosate and aminomethylphosphonic acid (AMPA), the principal glyphosate by-product, on chlorophyll metabolism and photosynthesis. Willow plants were treated with different concentrations of glyphosate (equivalent to 0, 1.4, 2.1 and 2.8kgha(-1)) and AMPA (equivalent to 0, 0.28, 1.4 and 2.8kgha(-1)) and evaluations of pigment contents, chlorophyll fluorescence, and oxidative stress markers (hydrogen peroxide content and antioxidant enzyme activities) in leaves were performed after 12h of exposure. We observed that AMPA and glyphosate trigger different mechanisms leading to decreases in chlorophyll content and photosynthesis rates in willow plants. Both chemicals induced ROS accumulation in willow leaves although only glyphosate-induced oxidative damage through lipid peroxidation. By disturbing chlorophyll biosynthesis, AMPA induced decreases in chlorophyll contents, with consequent effects on photosynthesis. With glyphosate, ROS increases were higher than the ROS-sensitive threshold, provoking chlorophyll degradation (as seen by pheophytin accumulation) and invariable decreases in photosynthesis. Peroxide accumulation in both AMPA and glyphosate-treated plants was due to the inhibition of antioxidant enzyme activities. The different effects of glyphosate on chlorophyll contents and photosynthesis as described in the literature may be due to various glyphosate:AMPA ratios in those plants. Copyright © 2015 Elsevier Inc. All rights reserved.

Multifractal analysis of oceanic chlorophyll maps remotely sensed from space

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L. de Montera

2011-03-01

Full Text Available Phytoplankton patchiness has been investigated with multifractal analysis techniques. We analyzed oceanic chlorophyll maps, measured by the SeaWiFS orbiting sensor, which are considered to be good proxies for phytoplankton. The study area is the Senegalo-Mauritanian upwelling region, because it has a low cloud cover and high chlorophyll concentrations. Multifractal properties are observed, from the sub-mesoscale up to the mesoscale, and are found to be consistent with the Corssin-Obukhov scale law of passive scalars. This result indicates that, in this specific region and within this scale range, turbulent mixing would be the dominant effect leading to the observed variability of phytoplankton fields. Finally, it is shown that multifractal patchiness can be responsible for significant biases in the nonlinear source and sink terms involved in biogeochemical numerical models.

Improving the Algae Bloom Prediction through the Assimilation of the Remotely Sensed Chlorophyll-A Data in a Generic Ecological Model in the North Sea

Science.gov (United States)

El Serafy, Ghada

2010-05-01

Harmful algae can cause damage to co-existing organisms, tourism and farmers. Accurate predictions of algal future composition and abundance as well as when and where algal blooms may occur could help early warning and mitigating. The Generic Ecological Model, GEM, [Blauw et al 2008] is an instrument that can be applied to any water system (fresh, transitional or coastal) to calculate the primary production, chlorophyll-a concentration and phytoplankton species composition. It consists of physical, chemical and ecological model components which are coupled together to build one generic and flexible modeling tool. For the North Sea, the model has been analyzed to assess sensitivity of the simulated chlorophyll-a concentration to a subset of ecologically significant set of factors. The research led to the definition of the most significant set of parameters to the algae blooming process in the North Sea [Salacinska et al 2009]. In order to improve the prediction of the model, the set of parameters and the chlorophyll-a concentration can be further estimated through the use of data assimilation. In this research, the Ensemble Kalman Filter (EnKF) data assimilation technique is used to assimilate the chlorophyll-a data of the North Sea, retrieved from MEdium Resolution Imaging Sensor (MERIS) spectrometer data [Peters et al 2005], in the GEM model. The chlorophyll-a data includes concentrations and error information that enable their use in data assimilation. For the same purpose, the uncertainty of the ecological generic model, GEM has been quantified by means of Monte Carlo approach. Through a study covering the year of 2003, the research demonstrates that both data and model are sufficiently robust for a successful assimilation. The results show that through the assimilation of the satellite data, a better description of the algae bloom has been achieved and an improvement of the capability of the model to predict the algae bloom for the North Sea has been confirmed

The power of pigments, calibrating chemoclines with chlorophylls and carotenoids.

Science.gov (United States)

Junium, C. K.; Uveges, B. T.

2017-12-01

Phototrophic organisms produce a diversity of pigments that serve a broad range of specific biochemical functions. Pigments are either directly associated with the photosynthetic apparatus, the most notable being chlorophyll a, or are accessory pigments such as the carotenoid lutein. Their functions can also be categorized into roles that are related to light harvesting (e.g. fucoxanthin) or for photoprotection (e.g. scytonemin). The abundances of these two classes of pigments from environmental samples can provide specific information about photointensity and how it relates to environmental changes. For example, a deepening of the chemo/nutricline can result in the increased production of light gathering relative to photoprotective pigments. Here we apply a relatively simple approach that utilizes the abundance of photosynthetic relative to photoprotective pigments to help constrain changes in the water column position of the chemocline. To test the efficacy of this approach we have utilized the sedimentary record of the anoxic Lake Kivu in the East African Rift. Recent Lake Kivu sediments are punctuated by a series of sapropels that may be associated with overturn of the lake, and release of carbon dioxide and sulfide during potential limnic eruptions. Carbon and nitrogen isotopes decrease significantly at the onset of sapropel deposition and suggest that 13C-depleted dissolved inorganic carbon was upwelled into surface waters and was accompanied by high concentrations of ammonium, that allowed for 15N-depletion during incomplete nitrogen utilization. The pigment record, specifically the ratio of the photoprotective carotenoids lutein and zeaxanthin to chlorophyll a increases significantly at the onset of sapropel deposition. This suggests that the chemocline shallowed, displacing phototrophic communities toward the surface of the lake where light intensities required production of photoprotective pigments. This approach can easily be applied to a wide variety of

Characterization of a Thermo-Inducible Chlorophyll-Deficient Mutant in Barley

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Rong Wang

2017-11-01

Full Text Available Leaf color is an important trait for not only controlling crop yield but also monitoring plant status under temperature stress. In this study, a thermo-inducible chlorophyll-deficient mutant, named V-V-Y, was identified from a gamma-radiated population of the barley variety Vlamingh. The leaves of the mutant were green under normal growing temperature but turned yellowish under high temperature in the glasshouse experiment. The ratio of chlorophyll a and chlorophyll b in the mutant declined much faster in the first 7–9 days under heat treatment. The leaves of V-V-Y turned yellowish but took longer to senesce under heat stress in the field experiment. Genetic analysis indicated that a single nuclear gene controlled the mutant trait. The mutant gene (vvy was mapped to the long arm of chromosome 4H between SNP markers 1_0269 and 1_1531 with a genetic distance of 2.2 cM and a physical interval of 9.85 Mb. A QTL for grain yield was mapped to the same interval and explained 10.4% of the yield variation with a LOD score of 4. This QTL is coincident with the vvy gene interval that is responsible for the thermo-inducible chlorophyll-deficient trait. Fine mapping, based on the barley reference genome sequence, further narrowed the vvy gene to a physical interval of 0.428 Mb with 11 annotated genes. This is the first report of fine mapping a thermo-inducible chlorophyll-deficient gene in barley.

STAY-GREEN and Chlorophyll Catabolic Enzymes Interact at Light-Harvesting Complex II for Chlorophyll Detoxification during Leaf Senescence in Arabidopsis[C][W

Science.gov (United States)

Sakuraba, Yasuhito; Schelbert, Silvia; Park, So-Yon; Han, Su-Hyun; Lee, Byoung-Doo; Andrès, Céline Besagni; Kessler, Felix; Hörtensteiner, Stefan; Paek, Nam-Chon

2012-01-01

During leaf senescence, plants degrade chlorophyll to colorless linear tetrapyrroles that are stored in the vacuole of senescing cells. The early steps of chlorophyll breakdown occur in plastids. To date, five chlorophyll catabolic enzymes (CCEs), NONYELLOW COLORING1 (NYC1), NYC1-LIKE, pheophytinase, pheophorbide a oxygenase (PAO), and red chlorophyll catabolite reductase, have been identified; these enzymes catalyze the stepwise degradation of chlorophyll to a fluorescent intermediate, pFCC, which is then exported from the plastid. In addition, STAY-GREEN (SGR), Mendel’s green cotyledon gene encoding a chloroplast protein, is required for the initiation of chlorophyll breakdown in plastids. Senescence-induced SGR binds to light-harvesting complex II (LHCII), but its exact role remains elusive. Here, we show that all five CCEs also specifically interact with LHCII. In addition, SGR and CCEs interact directly or indirectly with each other at LHCII, and SGR is essential for recruiting CCEs in senescing chloroplasts. PAO, which had been attributed to the inner envelope, is found to localize in the thylakoid membrane. These data indicate a predominant role for the SGR-CCE-LHCII protein interaction in the breakdown of LHCII-located chlorophyll, likely to allow metabolic channeling of phototoxic chlorophyll breakdown intermediates upstream of nontoxic pFCC. PMID:22366162

Chlorophyll_Master

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This data set included chlorophyll for each subregion in the study (Georges Bank, Gulf of Maine, Southern New England, Middle Atlantic Bight) . The data came from...

Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana.

Science.gov (United States)

Mork-Jansson, Astrid Elisabeth; Gargano, Daniela; Kmiec, Karol; Furnes, Clemens; Shevela, Dmitriy; Eichacker, Lutz Andreas

2015-10-07

The two-helix light harvesting like (Lil) protein Lil3 belongs to the family of chlorophyll binding light harvesting proteins of photosynthetic membranes. A function in tetrapyrrol synthesis and stabilization of geranylgeraniol reductase has been shown. Lil proteins contain the chlorophyll a/b-binding motif; however, binding of chlorophyll has not been demonstrated. We find that Lil3.2 from Arabidopsis thaliana forms heterodimers with Lil3.1 and binds chlorophyll. Lil3.2 heterodimerization (25±7.8 nM) is favored relative to homodimerization (431±59 nM). Interaction of Lil3.2 with chlorophyll a (231±49 nM) suggests that heterodimerization precedes binding of chlorophyll in Arabidopsis thaliana. Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Shellfish Aquaculture from Space: Potential of Sentinel2 to Monitor Tide-Driven Changes in Turbidity, Chlorophyll Concentration and Oyster Physiological Response at the Scale of an Oyster Farm

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Pierre Gernez

2017-05-01

Full Text Available The algorithms of Novoa et al. (2017 and Gons et al. (2005 were recalibrated and applied to Sentinel2 data to retrieve suspended particulate matter (SPM and chlorophyll a (chl a concentration in the environmentally and economically important intertidal zones. Sentinel2-derived chl a and SPM concentration distributions were analyzed at the scale of an oyster farm over a variety of tidal conditions. Sentinel2 imagery was then coupled with ecophysiological modeling to analyze the influence of tide-driven chl a and SPM dynamics on oyster clearance and chl consumption rates. Within the studied oyster farming site (Bourgneuf Bay along the French Atlantic coast, chl consumption rate mirrored the changes in chl a concentration during neap tides, whereas oyster clearance and chl consumption rates were both negatively impacted by high SPM concentration during spring tides.

How diffusivity, thermocline and incident light intensity modulate the dynamics of deep chlorophyll maximum in Tyrrhenian Sea.

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Davide Valenti

Full Text Available During the last few years theoretical works have shed new light and proposed new hypotheses on the mechanisms which regulate the spatio-temporal behaviour of phytoplankton communities in marine pelagic ecosystems. Despite this, relevant physical and biological issues, such as effects of the time-dependent mixing in the upper layer, competition between groups, and dynamics of non-stationary deep chlorophyll maxima, are still open questions. In this work, we analyze the spatio-temporal behaviour of five phytoplankton populations in a real marine ecosystem by using a one-dimensional reaction-diffusion-taxis model. The study is performed, taking into account the seasonal variations of environmental variables, such as light intensity, thickness of upper mixed layer and profiles of vertical turbulent diffusivity, obtained starting from experimental findings. Theoretical distributions of phytoplankton cell concentration was converted in chlorophyll concentration, and compared with the experimental profiles measured in a site of the Tyrrhenian Sea at four different times (seasons of the year, during four different oceanographic cruises. As a result we find a good agreement between theoretical and experimental distributions of chlorophyll concentration. In particular, theoretical results reveal that the seasonal changes of environmental variables play a key role in the phytoplankton distribution and determine the properties of the deep chlorophyll maximum. This study could be extended to other marine ecosystems to predict future changes in the phytoplankton biomass due to global warming, in view of devising strategies to prevent the decline of the primary production and the consequent decrease of fish species.

Chlorophyll-a retrieval in the Philippine waters

Science.gov (United States)

Perez, G. J. P.; Leonardo, E. M.; Felix, M. J.

2017-12-01

Satellite-based monitoring of chlorophyll-a (Chl-a) concentration has been widely used for estimating plankton biomass, detecting harmful algal blooms, predicting pelagic fish abundance, and water quality assessment. Chl-a concentrations at 1 km spatial resolution can be retrieved from MODIS onboard Aqua and Terra satellites. However, with this resolution, MODIS has scarce Chl-a retrieval in coastal and inland waters, which are relevant for archipelagic countries such as the Philippines. These gaps on Chl-a retrieval can be filled by sensors with higher spatial resolution, such as the OLI of Landsat 8. In this study, assessment of Chl-a concentration derived from MODIS/Aqua and OLI/Landsat 8 imageries across the open, coastal and inland waters of the Philippines was done. Validation activities were conducted at eight different sites around the Philippines for the period October 2016 to April 2017. Water samples filtered on the field were processed in the laboratory for Chl-a extraction. In situ remote sensing reflectance was derived from radiometric measurements and ancillary information, such as bathymetry and turbidity, were also measured. Correlation between in situ and satellite-derived Chl-a concentration using the blue-green ratio yielded relatively high R2 values of 0.51 to 0.90. This is despite an observed overestimation for both MODIS and OLI-derived values, especially in turbid and coastal waters. The overestimation of Chl-a may be attributed to inaccuracies in i) remote sensing reflectance (Rrs) retrieval and/or ii) empirical model used in calculating Chl-a concentration. However, a good 1:1 correspondence between the satellite and in situ maximum Rrs band ratio was established. This implies that the overestimation is largely due to the inaccuracies from the default coefficients used in the empirical model. New coefficients were then derived from the correlation analysis of both in situ-measured Chl-a concentration and maximum Rrs band ratio. This

Efficiency of a concentric matrix track detector surface scanning

International Nuclear Information System (INIS)

Bek-Uzarov, Dj.; Nikezic, D.; Kostic, D.; Krstic, D.; Cuknic, O.

1995-01-01

Heavy particle ionizing radiation track counting on the surface of a solid state round surface detector is made using the microscope and scanning step by step by a round field of vision. The whole solid state detector surface could not be fully or completely covered by round fields of visions. Therefore detector surface could be divided on the two parts, the larger surface, being under fields of vision, really scanned and no scanned missed or omitted surface. The ratio between omitted and scanned surfaces is so called track scanning efficiency. The knowledge of really counted, or scanned surface is a important value for evaluating the real surface track density an exposed solid state track detector. In the paper a matrix of a concentric field of vision made around the first microscope field of vision placed in center of the round disc of the scanned track detector is proposed. In a such scanning matrix the real scanned surface could be easy calculated and by the microscope scanning made as well. By this way scanned surface is very precisely obtained as well. Precise knowledge of scanned and omitted surface allows to obtain more precise scanning efficiency factor as well as real surface track density, the main parameter in solid state track detection measurements. (author)

A Study of Mixed Vegetable Dyes with Different Extraction Concentrations for Use as a Sensitizer for Dye-Sensitized Solar Cells

Directory of Open Access Journals (Sweden)

Kun-Ching Cho

2014-01-01

Full Text Available Two vegetable dyes are used for the study: chlorophyll dye from sweet potato leaf extract and anthocyanin dye from extracts of blueberry, purple cabbage, and grape. The chlorophyll and anthocyanin dyes are blended in a cocktail in equal proportions, by volume. This study determines the effect of different extraction concentrations and different vegetable dyes on the photoelectric conversion efficiency of dye-sensitized solar cells. In order to make the electrode for the experiments, P25 TiO2 powder was coated on the ITO conducting surface, using a medical blade, to form a thin film with a thickness of around 35 μm. The experimental results show that the cocktail dye blended using extracts of sweet potato leaf and blueberries, in the volumetric proportion 1 : 1, at a weight concentration of 40%, using an extraction temperature of 50°C and an extraction heating time of 10 min produces the greatest photoelectric conversion efficiency (η of up to 1.57%, an open-circuit voltage (VOC of 0.61 V, and a short-circuit current density (JSC of 4.75 mA/cm2.

The Characterization of Chlorophyll-A and Microalgae Isolation Process of Wastewater Collected at Sembrong Dam

Science.gov (United States)

Wellson, R.; Othman, N.; Matias-Peralta, H. M.

2016-07-01

Recently, there has been an increasing number of river water quality deterioration that has brought into water quality disruptions that entering dams including in Johor and one of them is occurred in Sembrong Dam in Johor. Sembrong Dam is a major water source for some 120,000 people in the districts of Kluang and parts of Batu Pahat. The quality of water in Sembrong should be well-monitored in ensuring the continuous distribution of clean and safe water supply to peoples. Based on the news reported by The Star news dated on 11 May 2015, the water bodies in Sembrong Dam are polluted by the algae blooms which has started to cause problems in treating water phase by clogging up the filters and causing the production to be reduced and finally resulting in frequent water disruptions to residents. Therefore, there is a need to study the water quality of the dam water prior to further water treatment. One of important characterizations is by measuring chlorophyll-a and the isolation of the dominant microalgae species in the water body in which they are able to indicate the level of water pollution. This paper presents the determination of chlorophyll-a and the isolation of microalgae strains collected from Sembrong Dam. Chlorophyll-a is a photosynthetic pigment present in all species of phytoplankton, including algae and in some photosynthetic bacteria, known as cyanobacteria. The method used in measuring the chlorophyll-a is based on the standard method of IS0 10 260. The average chlorophyll-a concentration measured at Sembrong Dam is 175.9 µg L-1 and it is responsible for the appearance of green color in the sample and it is categorized into hypereutrophic state which is highly polluted. The technique used for isolation of microalgae strains is traditional method which is by spreading the sample on agar. The pure isolate indicated that the genus Botryococcus is the dominant algae species which is characterized morphologically. Both chlorophyll-a and microalgae

Crystal Structure and Catalytic Mechanism of 7-Hydroxymethyl Chlorophyll a Reductase*

Science.gov (United States)

Wang, Xiao; Liu, Lin

2016-01-01

7-Hydroxymethyl chlorophyll a reductase (HCAR) catalyzes the second half-reaction in chlorophyll b to chlorophyll a conversion. HCAR is required for the degradation of light-harvesting complexes and is necessary for efficient photosynthesis by balancing the chlorophyll a/b ratio. Reduction of the hydroxymethyl group uses redox cofactors [4Fe-4S] cluster and FAD to transfer electrons and is difficult because of the strong carbon-oxygen bond. Here, we report the crystal structure of Arabidopsis HCAR at 2.7-Å resolution and reveal that two [4Fe-4S]clusters and one FAD within a very short distance form a consecutive electron pathway to the substrate pocket. In vitro kinetic analysis confirms the ferredoxin-dependent electron transport chain, thus supporting a proton-activated electron transfer mechanism. HCAR resembles a partial reconstruction of an archaeal F420-reducing [NiFe] hydrogenase, which suggests a common mode of efficient proton-coupled electron transfer through conserved cofactor arrangements. Furthermore, the trimeric form of HCAR provides a biological clue of its interaction with light-harvesting complex II. PMID:27072131

Chlorophyll catalyse the photo-transformation of carcinogenic benzo[a]pyrene in water

Science.gov (United States)

Luo, Lijuan; Lai, Xueying; Chen, Baowei; Lin, Li; Fang, Ling; Tam, Nora F. Y.; Luan, Tiangang

2015-01-01

Algal blooms cause great damage to water quality and aquaculture. However, this study showed that dead algal cells and chlorophyll could accelerate the photo-transformation of benzo[a]pyrene (BaP), a ubiquitous and persistent pollutant with potently mutagenic and carcinogenic toxicities, under visible light irradiation. Chlorophyll was found to be the major active substance in dead algal cells, and generated a high level of singlet oxygen to catalyse the photo-transformation of BaP. According to various BaP metabolites formed, the degradation mechanism was proposed as that chlorophyll in dead algal cells photo-oxidized BaP to quinones via photocatalytic generation of singlet oxygen. The results provided a good insight into the role of chlorophyll in the photo-transformation of organic contaminants and could be a possible remediation strategy of organic pollutants in natural environment. PMID:26239357

Continuous excitation chlorophyll fluorescence parameters: a review for practitioners.

Science.gov (United States)

Banks, Jonathan M

2017-08-01

This review introduces, defines and critically reviews a number of chlorophyll fluorescence parameters with specific reference to those derived from continuous excitation chlorophyll fluorescence. A number of common issues and criticisms are addressed. The parameters fluorescence origin (F0) and the performance indices (PI) are discussed as examples. This review attempts to unify definitions for the wide range of parameters available for measuring plant vitality, facilitating their calculation and use. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Radiation induced chlorophyll mutations in rice

International Nuclear Information System (INIS)

Bari, G.; Mustafa, G.; Soomro, A.M.; Baloch, A.W.

1985-01-01

Air dried grains of four local varieties of rice were treated with gamma-rays and fast neutrons for determining their mutagenic effectiveness through the occurence of chlorophyll mutations. Fast neutrons were more effective in inducing chlorophyll mutations and the rice variety Basmati 370 produced maximum number of mutations followed by varieties Sonahri Sugdasi, Jajai 77 and Sada Gulab. The highest frequency of chlorophyll mutations was that of albina types followed by striata types. The xantha, viridis and tigrina types of mutations were less frequent. (authors)

The dynamics of suspended particulate matter (SPM) and chlorophyll- a from intratidal to annual time scales in a coastal turbidity maximum

NARCIS (Netherlands)

van der Hout, C.M.; Witbaard, R.; Bergman, M.J.N.; Duineveld, G.C.A.; Rozemeijer, M.J.C.; Gerkema, T.

2017-01-01

The analysis of 1.8 years of data gives an understanding of the response to varying forcing of suspended particulate matter (SPM) and chlorophyll-a (CHL-a) in a coastal turbidity maximum zone (TMZ). Both temporal and vertical concentration variations in the near-bed layer (0–2 m) in the shallow (11

The dynamics of suspended particulate matter (SPM) and chlorophyll-a from intratidal to annual time scales in a coastal turbidity maximum

NARCIS (Netherlands)

Hout, van der C.M.; Witbaard, R.; Bergman, M.J.N.; Duineveld, G.C.A.; Rozemeijer, M.J.C.; Gerkema, T.

2017-01-01

The analysis of 1.8. years of data gives an understanding of the response to varying forcing of suspended particulate matter (SPM) and chlorophyll-a (CHL-a) in a coastal turbidity maximum zone (TMZ). Both temporal and vertical concentration variations in the near-bed layer (0-2. m) in the shallow

Kinetics and mechanism of the dissociation of chlorophyll and its metalloanalogues in proton-donating media

International Nuclear Information System (INIS)

Berezin, B.D.; Drobysheva, A.N.; Karmanova, L.P.

1976-01-01

The kinetics of the dissociation of chlorophyll a and its metalloanalogues (Zn 2+ and Cd 2+ complexes of chlorophyllic acid) have been investigated in t-butyl alcohol-trichloracetic acid mixtures. The dissociation reaction is kinetically firts-order with respect to the complex. The rate constants and the activation energies and entropies for the dissociation reaction have been calculated. In order to determine the order of the reaction with respect to the protogenic species, a study was made of the ionisation of m-nitroaniline in t-butyl alcohol at 25 0 C in the trichloroacetic acid concentration range from 0.15 to 4.75 M. The dissociation reaction of chlorophyll and its zinc-containing metalloanalogue has been shown to be of second order with respect to the solvated proton. The cadmium complex dissociates by a second-order reaction with respect to trichloroacetic acid

Chlorophyll Synthase under Epigenetic Surveillance Is Critical for Vitamin E Synthesis, and Altered Expression Affects Tocopherol Levels in Arabidopsis.

Science.gov (United States)

Zhang, Chunyu; Zhang, Wei; Ren, Guodong; Li, Delin; Cahoon, Rebecca E; Chen, Ming; Zhou, Yongming; Yu, Bin; Cahoon, Edgar B

2015-08-01

Chlorophyll synthase catalyzes the final step in chlorophyll biosynthesis: the esterification of chlorophyllide with either geranylgeranyl diphosphate or phytyl diphosphate (PDP). Recent studies have pointed to the involvement of chlorophyll-linked reduction of geranylgeranyl by geranylgeranyl reductase as a major pathway for the synthesis of the PDP precursor of tocopherols. This indirect pathway of PDP synthesis suggests a key role of chlorophyll synthase in tocopherol production to generate the geranylgeranyl-chlorophyll substrate for geranylgeranyl reductase. In this study, contributions of chlorophyll synthase to tocopherol formation in Arabidopsis (Arabidopsis thaliana) were explored by disrupting and altering expression of the corresponding gene CHLOROPHYLL SYNTHASE (CHLSYN; At3g51820). Leaves from the homozygous chlysyn1-1 null mutant were nearly devoid of tocopherols, whereas seeds contained only approximately 25% of wild-type tocopherol levels. Leaves of RNA interference lines with partial suppression of CHLSYN displayed marked reductions in chlorophyll but up to a 2-fold increase in tocopherol concentrations. Cauliflower mosaic virus35S-mediated overexpression of CHLSYN unexpectedly caused a cosuppression phenotype at high frequencies accompanied by strongly reduced chlorophyll content and increased tocopherol levels. This phenotype and the associated detection of CHLSYN-derived small interfering RNAs were reversed with CHLSYN overexpression in rna-directed rna polymerase6 (rdr6), which is defective in RNA-dependent RNA polymerase6, a key enzyme in sense transgene-induced small interfering RNA production. CHLSYN overexpression in rdr6 had little effect on chlorophyll content but resulted in up to a 30% reduction in tocopherol levels in leaves. These findings show that altered CHLSYN expression impacts tocopherol levels and also, show a strong epigenetic surveillance of CHLSYN to control chlorophyll and tocopherol synthesis. © 2015 American Society of

Xanthophyll cycle-dependent quenching of photosystem II chlorophyll a fluorescence: Formation of a quenching complex with a short fluorescence lifetime

Energy Technology Data Exchange (ETDEWEB)

Gilmore, A.M.; Hazlett, T.L.; Govindjee [Univ. of Illinois, Urbana, IL (United States)

1995-03-14

Excess light triggers protective nonradiative dissipation of excitation energy in photosystem II through the formation of a trans-thylakoid pH gradient that in turn stimulates formation of zeaxanthin and antheraxanthin. These xanthophylls when combined with protonation of antenna pigment-protein complexes may increase nonradiative dissipation and, thus, quench chlorophyll a fluorescence. Here we measured, in parallel, the chlorophyll a fluorescence lifetime and intensity to understand the mechanism of this process. Increasing the xanthophyll concentration in the presence of a pH gradient (quenched conditions) decreases the fractional intensity of a fluorescence lifetime component centered at {approx}2 ns and increases a component at {approx}0.4 ns. Uncoupling the pH gradient (unquenched conditions) eliminates the 0.4-ns component. Changes in the xanthophyll concentration do not significantly affect the fluorescence lifetimes in either the quenched or unquenched sample conditions. However, there are differences in fluorescence lifetimes between the quenched and unquenched states that are due to pH-related, but nonxanthophyll-related, processes. Quenching of the maximal fluorescence intensity correlates with both the xanthophyll concentration and the fractional intensity of the 0.4-ns component. The unchanged fluorescence lifetimes and the proportional quenching of the maximal and dark-level fluorescence intensities indicate that the xanthophyllact on antenna, not reaction center processes. Further, the fluorescence quenching is interpreted as the combined effect of the pH gradient and xanthophyll concentration, resulting in the formation of a quenching complex with a short ({approx}0.4 ns) fluorescence lifetime. 33 refs., 6 figs., 2 tabs.

Variability of mesozooplankton biomass and individual size in a coast-offshore transect in the Catalan Sea: relationships with chlorophyll a and hydrographic features

KAUST Repository

Alcaraz, Miquel; Calbet, Albert; Isari, Stamatina; Irigoien, Xabier; Trepat, Isabel; Saiz, Enric

2016-01-01

The temporal and spatial changes of zooplankton and chlorophyll a concentration were studied during the warm stratification period (early June) at three stations whose traits corresponded to the coastal, frontal, and offshore-dome water conditions described for the Catalan Sea. We sampled the stations for 12 days at a frequency ranging from less than 10 to 102 h, with a spatial resolution ranging from 10 to 104 m. The objective was to determine the variability of mesozooplankton and phytoplankton (chlorophyll a) biomass, and average individual size (mass) across a coast-offshore transect in relation to the stratification conditions prevailing in the NW Mediterranean during summer. The vertical distribution of phytoplankton biomass displayed a clear deep maximum at 60 m depth except at the coastal station. This maximum exists during most of the year and is especially important during the density stratification period. It was accompanied during daylight hours by a coherent zooplankton maximum. At sunset mesozooplankton ascended and dispersed, with larger organisms from deeper layers joining the migrating community and increasing the average individual mass. The highest variability of mesozooplankton biomass, individual mass and chlorophyll a concentration occurred at the front station due to the coupling between the vertical migration of zooplankton and the particular characteristics of the front. According to the data shown, the highest variability was observed at the lowest scales.

Variability of mesozooplankton biomass and individual size in a coast-offshore transect in the Catalan Sea: relationships with chlorophyll a and hydrographic features

KAUST Repository

Alcaraz, Miquel

2016-10-11

The temporal and spatial changes of zooplankton and chlorophyll a concentration were studied during the warm stratification period (early June) at three stations whose traits corresponded to the coastal, frontal, and offshore-dome water conditions described for the Catalan Sea. We sampled the stations for 12 days at a frequency ranging from less than 10 to 102 h, with a spatial resolution ranging from 10 to 104 m. The objective was to determine the variability of mesozooplankton and phytoplankton (chlorophyll a) biomass, and average individual size (mass) across a coast-offshore transect in relation to the stratification conditions prevailing in the NW Mediterranean during summer. The vertical distribution of phytoplankton biomass displayed a clear deep maximum at 60 m depth except at the coastal station. This maximum exists during most of the year and is especially important during the density stratification period. It was accompanied during daylight hours by a coherent zooplankton maximum. At sunset mesozooplankton ascended and dispersed, with larger organisms from deeper layers joining the migrating community and increasing the average individual mass. The highest variability of mesozooplankton biomass, individual mass and chlorophyll a concentration occurred at the front station due to the coupling between the vertical migration of zooplankton and the particular characteristics of the front. According to the data shown, the highest variability was observed at the lowest scales.

Induced mutations in chickpea (Cicer arietinum L.) II. frequency and spectrum of chlorophyll mutations

International Nuclear Information System (INIS)

Kharkwal, M.C.

1998-01-01

A comparative study of frequency and spectrum of chlorophyll mutations induced by two physical (gamma rays, fast neutrons) and two chemical mutagens (NMU, EMS) in relation to the effects in M1 plants and induction of mutations in M2 was made in four chickpea (Cicer arietinum L.) varieties, two desi (G 130 & H 214) one Kabuli (C 104) and one green seeded (L 345). The treatments included three doses each of gamma rays (400, 500 & 600 Gy) and fast neutrons (5, 10 & 15 Gy) and two concentrations with two different durations of two chemical mutagens, NMU [0.01% (20h), & 0.02% (8h)] and EMS [0.1% (20h) & 0.2% (8h)]. The frequencies and spectrum of three different kinds of induced chlorophyll mutations in the order albina (43.5%), chlorina (27.3%) and xantha (24.2%) were recorded. Chemical mutagens were found to be efficient in inducing chlorophyll mutations in chickpea. Highest frequency of mutations was observed in green seeded var. L 345 (83% of M1 families and 19.9/1000 M2 plants). Kabuli var. C 104 was least responsive for chlorophyll mutations

Estimating chlorophyll with thermal and broadband multispectral high resolution imagery from an unmanned aerial system using relevance vector machines for precision agriculture

Science.gov (United States)

Elarab, Manal; Ticlavilca, Andres M.; Torres-Rua, Alfonso F.; Maslova, Inga; McKee, Mac

2015-12-01

Precision agriculture requires high-resolution information to enable greater precision in the management of inputs to production. Actionable information about crop and field status must be acquired at high spatial resolution and at a temporal frequency appropriate for timely responses. In this study, high spatial resolution imagery was obtained through the use of a small, unmanned aerial system called AggieAirTM. Simultaneously with the AggieAir flights, intensive ground sampling for plant chlorophyll was conducted at precisely determined locations. This study reports the application of a relevance vector machine coupled with cross validation and backward elimination to a dataset composed of reflectance from high-resolution multi-spectral imagery (VIS-NIR), thermal infrared imagery, and vegetative indices, in conjunction with in situ SPAD measurements from which chlorophyll concentrations were derived, to estimate chlorophyll concentration from remotely sensed data at 15-cm resolution. The results indicate that a relevance vector machine with a thin plate spline kernel type and kernel width of 5.4, having LAI, NDVI, thermal and red bands as the selected set of inputs, can be used to spatially estimate chlorophyll concentration with a root-mean-squared-error of 5.31 μg cm-2, efficiency of 0.76, and 9 relevance vectors.

Numerical simulation of the double pits stress concentration in a curved casing inner surface

Directory of Open Access Journals (Sweden)

Wei Yan

2016-12-01

Full Text Available Sour or sweet oil fields development is common in recent years. Casing and tubing are usually subjected to pitting corrosion because of exposure to the strong corrosion species, such as CO2, H2S, and saline water. When the corrosion pits formed in the casing inner surface, localized stress concentration will occur and the casing strength will be degraded. Thus, it is essential to evaluate the degree of stress concentration factor accurately. This article performed a numerical simulation on double pits stress concentration factor in a curved inner surface using the finite element software ABAQUS. The results show that the stress concentration factor of double pits mainly depends on the ratio of two pits distance to the pit radius (L/R. It should not be only assessed by the absolute distance between the two pits. When the two pits are close and tangent, the maximum stress concentration factor will appear on the inner tangential edges. Stress concentration increased by double pits in a curved casing inner surface is more serious than that in a flat surface. A correction factor of 1.9 was recommended in the curved inner surface double pits stress concentration factor predict model.

Isopleths of surface air concentration and surface air kerma rate due to a radioactive cloud released from a stack (3)

International Nuclear Information System (INIS)

Tachibana, Haruo; Kikuchi, Masamitsu; Sekita, Tsutomu; Yamaguchi, Takenori

2004-06-01

This report is a revised edition of 'Isopleths of Surface Air Concentration and Surface Air Absorbed Dose Rate due to a Radioactive Cloud Released from a Stack(II) '(JAERI-M 90-206) and based on the revised Nuclear Safety Guidelines reflected the ICRP1990 Recommendation. Characteristics of this report are the use of Air Karma Rate (Gy/h) instead of Air Absorbed Dose Rate (Gy/h), and the record of isopleths of surface air concentration and surface air karma rate on CD-ROM. These recorded data on CD-ROM can be printed out on paper and/or pasted on digital map by personal computer. (author)

Correlation of electronic carotenoid-chlorophyll interactions and fluorescence quenching with the aggregation of native LHC II and chlorophyll deficient mutants

International Nuclear Information System (INIS)

Liao, Pen-Nan; Bode, Stefan; Wilk, Laura; Hafi, Nour; Walla, Peter J.

2010-01-01

The aggregation dependent correlation between fluorescence quenching and the electronic carotenoid-chlorophyll interactions, φ Coupling Car S 1 -Chl , as measured by comparing chlorophyll fluorescence observed after two- and one-photon excitation, has been investigated using native LHC II samples as well as mutants lacking Chl 2 and Chl 13. For native LHC II the same linear correlation between φ Coupling Car S 1 -Chl and the fluorescence quenching was observed as previously reported for the pH and Zea-dependent quenching of LHC II . In order to elucidate which carotenoid-chlorophyll pair might dominate this correlation we also investigated the mutants lacking Chl 2 and Chl 13. However, also with these mutants the same linear correlation as for native LHC II was observed. This provides indication that these two chlorophylls play only a minor role for the observed effects. Nevertheless, we also conclude that this does not exclude that their neighboured carotenoids, lutein 1 and neoxanthin, might interact electronically with other chlorophylls close by.

Characterisation of chlorophyll oxidation mediated by peroxidative activity in olives (Olea europaea L.) cv. Hojiblanca.

Science.gov (United States)

Vergara-Domínguez, Honorio; Roca, María; Gandul-Rojas, Beatriz

2013-08-15

The oxidation of chlorophyll a (chl a) catalysed by peroxidase (POD) from mesocarp of the olive fruit (Olea europaea L., cv Hojiblanca) in the presence of H2O2 and 2,4-dichlorophenol (2,4-DCP), is characterised via the individualised quantification of the products of the enzymatic reaction using a new methodology of HPLC-UV spectrometry. This innovation has allowed the discovery that, in addition to 13(2) OH chl a and 15(1) OH lactone chl a, which are the first products of POD on chl a, the reaction process sequentially creates another series of oxidised chlorophyll derivatives which have not been previously described. Their origins have been linked to POD activity in the presence of 2,4-DCP. Likewise, a study of the effect of the concentration of the various cosubstrates on the POD reaction rate demonstrated that the correct establishment of the relative concentrations of the same ([H2O2]/[2,4-DCP]/[Chl]=1:3:0.02) is crucial to explaining inhibition effects by substrates and carrying out optimum measurements. Therefore, new essential parameters for the determination of POD activity on a chlorophyll substrate are established. Copyright © 2013 Elsevier Ltd. All rights reserved.

Satellite remote sensing of chlorophyll a in support of nutrient management in the Neuse and Tar-Pamlico River (North Carolina) estuaries

Science.gov (United States)

The North Carolina Environmental Management Commission (EMC) has adopted as a water quality standard that chlorophyll a concentration should not exceed 40 ug/L in sounds, estuaries and other slow-moving waters. Exceedances require regulators to develop a Total Maximum Daily Limit...

Helicopter-based lidar system for monitoring the upper ocean and terrain surface

International Nuclear Information System (INIS)

Lee, Kwi Joo; Park, Youngsik; Bunkin, Alexey; Pershin, Serguei; Voliak, Konstantin; Nunes, Raul

2002-01-01

A compact helicopter-based lidar system is developed and tested under laboratory and field conditions. It is shown that the lidar can measure concentrations of chlorophyll a and dissolved organic matter at the surface of water bodies, detect fluorescence spectra of ground vegetation at a distance of up to 530 m, and determine the vertical profile of light-scattering particle concentration in the upper ocean. The possibilities of the lidar system are demonstrated by detection of polluted areas at the ocean surface, by online monitoring of three-dimensional distribution of light-scattering layers, and by recognition of plant types and physiological states

Cylindrical aggregates of chlorophylls studied by small-angle neutron scatter

Energy Technology Data Exchange (ETDEWEB)

Worcester, D.L. [Univ. of Missouri, Columbus, MO (United States); Katz, J.J. [Argonne National Laboratory, IL (United States)

1994-12-31

Neutron small-angle scattering has demonstrated tubular chlorophyll aggregates formed by self-assembly of a variety of chlorophyll types in nonpolar solvents. The size and other properties of the tubular aggregates can be accounted for by stereochemical properties of the chlorophyll molecules. Features of some of the structures are remarkably similar to light harvesting chlorophyll complexes in vivo, particularly for photosynthetic bacteria. These nanotube chlorophyll structures may have applications as light harvesting biomaterials where efficient energy transfer occurs from an excited state which is highly delocalized.

Calibrations between chlorophyll meter values and chlorophyll contents vary as the result of differences in leaf structure

Science.gov (United States)

In order to relate leaf chlorophyll meter values with total leaf chlorophyll contents (µg cm-2), calibration equations are established with measured data on leaves. Many studies have documented differences in calibration equations using different species and using different growing conditions for th...

Notes on the instability of extracted chlorophyll and a reported effect of ozone on lichen algae

Energy Technology Data Exchange (ETDEWEB)

Brown, D H

1980-01-01

Recently Rosentreter and Ahmadjian reported on the effects of ozone on the chlorophyll content of Cladonia arbuscula and isolated Trebouxia cells. However, the chlorophyll data they presented, even for the control, unozone-treated material, appears to be unusual. They reported values of the chlorophyll a/b ratio of 0.308-0.463 from Cladonia arbuscula and 0.345-0.476 for Trebouxia which are substantially lower than other published values. Because chlorophyll b is normally present in green plants as a minor accessory photosynthetic pigment, the suggestion that it was the major chlorophyll component requires some explanation. The purpose of this study is to show that chlorophyll is unstable when extracted into methanol and to suggest that the extraction and storage conditions used by Rosentreter and Ahmadjian may have allowed pigment degradation to occur which thereby accounted for the low chlorophyll a/b ratios they obtained.

Contribution of Polyphenol Oxidation, Chlorophyll and Vitamin C Degradation to the Blackening of Piper nigrum L.

Science.gov (United States)

Gu, Fenglin; Huang, Feifei; Wu, Guiping; Zhu, Hongying

2018-02-09

Black pepper ( Piper nigrum L.) is the most widely used spice in the world. Blackening is considered to be beneficial and important in the processing of black pepper because it contributes to its color and flavor. The purpose of this paper is to investigate polyphenol oxidation as well as the chlorophyll and vitamin C (VC) degradation in the blackening of Piper nigrum L. Black pepper was produced by four methods, and changes in polyphenols, chlorophyll and VC were studied by high performance liquid chromatography (HPLC) and ultraviolet-visible and visible (UV-Vis) spectrophotometry. The results show that polyphenol oxidase activity significantly decreased during the preparation of black pepper, and the concentrations of phenolic compounds, VC, and chlorophyll a and b also significantly decreased. Polyphenol oxidation and chlorophyll and VC degradation contribute to the blackening. A crude extract of phenolic compounds from black pepper was prepared by the system solvent method. The greater the polarity of the extraction solvent, the higher the extraction rates of the phenolic compounds and the total phenol content. Pepper phenolic compounds were analyzed by HPLC analysis.

Contribution of Polyphenol Oxidation, Chlorophyll and Vitamin C Degradation to the Blackening of Piper nigrum L.

Directory of Open Access Journals (Sweden)

Fenglin Gu

2018-02-01

Full Text Available Black pepper (Piper nigrum L. is the most widely used spice in the world. Blackening is considered to be beneficial and important in the processing of black pepper because it contributes to its color and flavor. The purpose of this paper is to investigate polyphenol oxidation as well as the chlorophyll and vitamin C (VC degradation in the blackening of Piper nigrum L. Black pepper was produced by four methods, and changes in polyphenols, chlorophyll and VC were studied by high performance liquid chromatography (HPLC and ultraviolet-visible and visible (UV-Vis spectrophotometry. The results show that polyphenol oxidase activity significantly decreased during the preparation of black pepper, and the concentrations of phenolic compounds, VC, and chlorophyll a and b also significantly decreased. Polyphenol oxidation and chlorophyll and VC degradation contribute to the blackening. A crude extract of phenolic compounds from black pepper was prepared by the system solvent method. The greater the polarity of the extraction solvent, the higher the extraction rates of the phenolic compounds and the total phenol content. Pepper phenolic compounds were analyzed by HPLC analysis.

Thermal-based modeling of coupled carbon, water, and energy fluxes using nominal light use efficiencies constrained by leaf chlorophyll observations

KAUST Repository

Schull, M. A.

2015-03-11

Recent studies have shown that estimates of leaf chlorophyll content (Chl), defined as the combined mass of chlorophyll a and chlorophyll b per unit leaf area, can be useful for constraining estimates of canopy light use efficiency (LUE). Canopy LUE describes the amount of carbon assimilated by a vegetative canopy for a given amount of absorbed photosynthetically active radiation (APAR) and is a key parameter for modeling land-surface carbon fluxes. A carbon-enabled version of the remote-sensing-based two-source energy balance (TSEB) model simulates coupled canopy transpiration and carbon assimilation using an analytical sub-model of canopy resistance constrained by inputs of nominal LUE (βn), which is modulated within the model in response to varying conditions in light, humidity, ambient CO2 concentration, and temperature. Soil moisture constraints on water and carbon exchange are conveyed to the TSEB-LUE indirectly through thermal infrared measurements of land-surface temperature. We investigate the capability of using Chl estimates for capturing seasonal trends in the canopy βn from in situ measurements of Chl acquired in irrigated and rain-fed fields of soybean and maize near Mead, Nebraska. The results show that field-measured Chl is nonlinearly related to βn, with variability primarily related to phenological changes during early growth and senescence. Utilizing seasonally varying βn inputs based on an empirical relationship with in situ measured Chl resulted in improvements in carbon flux estimates from the TSEB model, while adjusting the partitioning of total water loss between plant transpiration and soil evaporation. The observed Chl-βn relationship provides a functional mechanism for integrating remotely sensed Chl into the TSEB model, with the potential for improved mapping of coupled carbon, water, and energy fluxes across vegetated landscapes.

Plant leaf chlorophyll content retrieval based on a field imaging spectroscopy system.

Science.gov (United States)

Liu, Bo; Yue, Yue-Min; Li, Ru; Shen, Wen-Jing; Wang, Ke-Lin

2014-10-23

A field imaging spectrometer system (FISS; 380-870 nm and 344 bands) was designed for agriculture applications. In this study, FISS was used to gather spectral information from soybean leaves. The chlorophyll content was retrieved using a multiple linear regression (MLR), partial least squares (PLS) regression and support vector machine (SVM) regression. Our objective was to verify the performance of FISS in a quantitative spectral analysis through the estimation of chlorophyll content and to determine a proper quantitative spectral analysis method for processing FISS data. The results revealed that the derivative reflectance was a more sensitive indicator of chlorophyll content and could extract content information more efficiently than the spectral reflectance, which is more significant for FISS data compared to ASD (analytical spectral devices) data, reducing the corresponding RMSE (root mean squared error) by 3.3%-35.6%. Compared with the spectral features, the regression methods had smaller effects on the retrieval accuracy. A multivariate linear model could be the ideal model to retrieve chlorophyll information with a small number of significant wavelengths used. The smallest RMSE of the chlorophyll content retrieved using FISS data was 0.201 mg/g, a relative reduction of more than 30% compared with the RMSE based on a non-imaging ASD spectrometer, which represents a high estimation accuracy compared with the mean chlorophyll content of the sampled leaves (4.05 mg/g). Our study indicates that FISS could obtain both spectral and spatial detailed information of high quality. Its image-spectrum-in-one merit promotes the good performance of FISS in quantitative spectral analyses, and it can potentially be widely used in the agricultural sector.

Effects of tributyltin on the chlorophyll contents of marine microalga Tetraselmis tetrathele, Nannochloropsis oculata and Dunaliella sp

OpenAIRE

Rumampuk, Natalie D. C.; Rumengan, Inneke F. M.; Ohji, Madoka; Arai, Takaomi; Miyazaki, Nobuyuki

2004-01-01

Three species of marine microalga Tetraselmis tetrathele, Nannochloropsis oculata and Dunaliella sp., the important phyto-plankton in many hatcheries, were used in an acute toxicity test of TBT with emphasis on the chlorophyll a and b contents. After a very short exposure time of three concentrations of TBT, 0.1, 0.5 and 1μgl^, the three species of algae showed different responses. The chlorophyll a and b contents of T. tetrathele in all treatments were higher than those in controls, even be ...

Analysis of chlorophyll pigment concentration in the northern Arabian Sea using remote sensing and GIS

Digital Repository Service at National Institute of Oceanography (India)

Thapa, S.

hits a phytoplankton, all of the li absorbed. All light, which is not abso back to the human eye, which is w Chlorophyll a absorbs maximally at 440 and 675nm. Using this principle of light absorption by phytoplankton, it is possible to detect them... and lanning, transportation and infrastructure planning, market analysis, visual pact analysis, facilities management, tax assessment, real estate analysis nd many other applications.�1�1 .2 FUNCTIONS OF GIS ata entry ata display ata management ata Output .3...

Studying of Salinity Stress Effect on Germination, Proline, Sugar, Protein, Lipid and Chlorophyll Content in Purslane (Portulaca oleracea L. Leaves

Directory of Open Access Journals (Sweden)

Parvaneh Rahdari

2012-04-01

Full Text Available Approximately 30% of country regions are under influence of salt and one of the main important methods for increasing of hectare performance in salt soils, using of resistant plant against with salt such as medicinal annual plant of Purslane (Portulaca oleraceae L..Studying of the salt stress effect on germination and some physiological parameters in Purslane was carried out in completely random framework and with three replications and under controlled conditions. In this study, salinity was imposed by sodium chloride (NaCl and in five levels of framework included of Control (distilled water, 50, 100, 150 and 200 Mmol from NaCl. Studied physiological parameters included of proline, sugar, protein, lipid and chlorophyll in Purslane leaves.In relation with observed germination that increasing of salt level, germination rate was reduced that the least germination rate in 200 Mmol and the highest germination was observed in control which in statistical level 0.01 showed meaningful difference. Also, results showed an increasing of leaf proline and sugar that was synchronize with increasing of sodium chloride, this increasing in statistical level of P<0.01 was meaningful, while blank had the least rate and salt level 200 Mmol was observed. Synchronize with increasing of salinity level, lipid and protein concentration decreasing that was meaningful (P<0.01 which highest of leaf lipid and protein content in control level and least concentration was observed in 200 Mmol of salinity level. Also, salinity stress due to increasing of chlorophyll a and b levels in Purslane leaves which this increasing in leaf chlorophyll b concentrations in 0.01 statistical level was meaningful and the least level chlorophyll a and b in blank and the highest level of chlorophyll a and b by order in 150 and 200 Mmol of NaCl was observed. The results showed that plant for resistance against salinity increasing of proline, sugar and chlorophyll content in leaves are useful for

The chemical bonds effect of anthocyanin and chlorophyll dyes on TiO2 for dye-sensitized solar cell (DSSC)

Science.gov (United States)

Ahliha, A. H.; Nurosyid, F.; Supriyanto, A.; Kusumaningsih, T.

2017-11-01

Anthocyanin and chlorophyll dyes have been blended as the photosensitizer of Dye-Sensitized Solar Cell (DSSC). The results study showed the effect of chemical bond dyes on TiO2 and the efficiency of DSSC. Ratio blend of the anthocyanin and chlorophyll dyes are 1:1. The absorbance of dyes and TiO2 were characterized using UV-Vis Spectrophotometer. The chemical bonds contained in TiO2-dyes were characterized using FT-IR spectrophotometer. The efficiency of DSSC was calculated using I-V meter. The absorption spectra of chlorophyll: anthocyanin blend dye solutions and TiO2 films can increase after the dye adsorption. Absorbance characterization of anthocyanin and chlorophyll dye blend solutions showed three peaks at the wavelength of 412 nm; 535.5 nm; and 656.5 nm. Absorbance characterization of spinach before being blend with anthocyanin dyes solutions showed two peaks at the wavelength of 431 nm and 665.5 nm. The absorption spectra of TiO2 films can increase after the dyes adsorption at the wavelength of 400 nm. FT-IR spectra of TiO2 founded the functional groups C-Br, C=C, and O-H. The functional groups founded in anthocyanin: chlorophyll dye blended on the surface of TiO2 are C-Br, C-O, O-H, C-H, C=C, C=O, and O-H. The result showed that the greatest efficiency of 0.0544% at dye red cabbage-spinach. Adsorption blends of anthocyanin and chlorophyll dyes on the surface of TiO2 can be used as the photosensitizer for DSSC.

Interannual variability of chlrophyll concentration in the eastern Arabian Sea

Digital Repository Service at National Institute of Oceanography (India)

Karanje, S.

.325 Chlorophyll Conce n tra t ion (m g/m * *3) 0.2 0.29 0.28 0.26 0.26 0.23 0.26 0.22 0.3 0.25 0.075 0.091 0.097 0.09 0.098 0.079 0.088 0.07 0.085 0.087 Chlorophyll Concentration (January)1998-2007 Legend Mean_Plot 1 SD_Plot 2 Mean_Fit 1: Linear Period... Mean_Fit 1: Linear Chlorophyll Concentration (February) 1998-2007 Monthly mean chlorophyll in March during 1998 to 2007 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 Year 0.025 0.05 0.075 0.1 0.125 0.15 0.175 0.2 0.225 0.25 C h l o ro p...

Oceanographic profile temperature, chlorophyll and other measurements collected using bottle from the SHIRASE (JSVY) in the Antarctic from 1984 to 1985 (NODC Accession 0001048)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Source: Temperature, chlorophyll and other profile data received at NODC on 04/01/03 by Todd O'Brien from "Fukuda, Y., M. Ohno, M. Fukuchi, 1986 "Surface Chlorophyll...

Dislocation behavior of surface-oxygen-concentration controlled Si wafers

International Nuclear Information System (INIS)

Asazu, Hirotada; Takeuchi, Shotaro; Sannai, Hiroya; Sudo, Haruo; Araki, Koji; Nakamura, Yoshiaki; Izunome, Koji; Sakai, Akira

2014-01-01

We have investigated dislocation behavior in the surface area of surface-oxygen-concentration controlled Si wafers treated by a high temperature rapid thermal oxidation (HT-RTO). The HT-RTO process allows us to precisely control the interstitial oxygen concentration ([O i ]) in the surface area of the Si wafers. Sizes of rosette patterns, generated by nano-indentation and subsequent thermal annealing at 900 °C for 1 h, were measured for the Si wafers with various [O i ]. It was found that the rosette size decreases in proportion to the − 0.25 power of [O i ] in the surface area of the Si wafers, which were higher than [O i ] of 1 × 10 17 atoms/cm 3 . On the other hand, [O i ] of lower than 1 × 10 17 atoms/cm 3 did not affect the rosette size very much. These experimental results demonstrate the ability of the HT-RTO process to suppress the dislocation movements in the surface area of the Si wafer. - Highlights: • Surface-oxygen-concentration controlled Si wafers have been made. • The oxygen concentration was controlled by high temperature rapid thermal oxidation. • Dislocation behavior in the surface area of the Si wafers has been investigated. • Rosette size decreased with increasing of interstitial oxygen atoms. • The interstitial oxygen atoms have a pinning effect of dislocations at the surface

Efficiency of chlorophyll in gross primary productivity: A proof of concept and application in crops.

Science.gov (United States)

Gitelson, Anatoly A; Peng, Yi; Viña, Andrés; Arkebauer, Timothy; Schepers, James S

2016-08-20

One of the main factors affecting vegetation productivity is absorbed light, which is largely governed by chlorophyll. In this paper, we introduce the concept of chlorophyll efficiency, representing the amount of gross primary production per unit of canopy chlorophyll content (Chl) and incident PAR. We analyzed chlorophyll efficiency in two contrasting crops (soybean and maize). Given that they have different photosynthetic pathways (C3 vs. C4), leaf structures (dicot vs. monocot) and canopy architectures (a heliotrophic leaf angle distribution vs. a spherical leaf angle distribution), they cover a large spectrum of biophysical conditions. Our results show that chlorophyll efficiency in primary productivity is highly variable and responds to various physiological and phenological conditions, and water availability. Since Chl is accessible through non-destructive, remotely sensed techniques, the use of chlorophyll efficiency for modeling and monitoring plant optimization patterns is practical at different scales (e.g., leaf, canopy) and under widely-varying environmental conditions. Through this analysis, we directly related a functional characteristic, gross primary production with a structural characteristic, canopy chlorophyll content. Understanding the efficiency of the structural characteristic is of great interest as it allows explaining functional components of the plant system. Copyright © 2016 Elsevier GmbH. All rights reserved.

Spatial variability of chlorophyll and nitrogen content of rice from hyperspectral imagery

Science.gov (United States)

Moharana, Shreedevi; Dutta, Subashisa

2016-12-01

Chlorophyll and nitrogen are the most essential parameters for paddy crop growth. Spectroradiometric measurements were collected at canopy level during critical growth period of rice. Chemical analysis was performed to quantify the total leaf content. By exploiting the ground based measurements, regression models were established for chlorophyll and nitrogen aimed indices with their corresponding crop growth variables. Vegetation index models were developed for mapping these parameters from Hyperion imagery in an agriculture system. It was inferred that the present Simple Ratio (SR) and Leaf Nitrogen Concentration (LNC) indices, which followed a linear and nonlinear relationship respectively, were completely different from published Tian et al. (2011). The nitrogen content varied widely from 1 to 4% and only 2 to 3% for paddy crop using present modified index models and Tian et al. (2011) respectively. The modified LNC index model performed better than the established Tian et al. (2011) model as far as estimated nitrogen content from Hyperion imagery was concerned. Furthermore, within the observed chlorophyll range obtained from the studied rice varieties grown in the rice agriculture system, the index models (LNC, OASVI, Gitelson, mSR and MTCI) performed well in the spatial distribution of rice chlorophyll content from Hyperion imagery. Spatial distribution of total chlorophyll content varied widely from 1.77 to 5.81 mg/g (LNC), 3.0 to 13 mg/g (OASVI), 0.5 to 10.43 mg/g (Gitelson), 2.18 to 10.61 mg/g (mSR) and 2.90 to 5.40 mg/g (MTCI). The spatial information of these parameters will help in proper nutrient management, yield forecasting, and will serve as inputs for crop growth and forecasting models for a precision rice agriculture system.

The most oligotrophic subtropical zones of the global ocean: similarities and differences in terms of chlorophyll and yellow substance

OpenAIRE

A. Morel; H. Claustre; B. Gentili

2010-01-01

The cores of the subtropical anticyclonic gyres are characterized by their oligotrophic status and minimal chlorophyll concentration, compared to that of the whole ocean. These zones are unambiguously detected by space borne ocean color sensors thanks to their typical spectral reflectance, which is that of extremely clear and deep blue waters. Not only the low chlorophyll (denoted [Chl]) level, but also a reduced amount of colored dissolved organic matter (CDOM or "yellow substance") account ...

The most oligotrophic subtropical zones of the global ocean: similarities and differences in terms of chlorophyll and yellow substance

OpenAIRE

Morel, A.; Claustre, H.; Gentili, B.

2010-01-01

The cores of the subtropical anticyclonic gyres are characterized by their oligotrophic status and minimal chlorophyll concentration, compared to that of the whole ocean. These zones are unambiguously detected by space borne ocean color sensors thanks to their typical spectral reflectance, which is that of extremely clear and deep blue waters. Not only the low chlorophyll (denoted [Chl]) level, but also a reduced amount of colored dissolved organic matter (CDOM or "yellow substance") acc...

Comparison of satellite reflectance algorithms for estimating chlorophyll-a in a temperate reservoir using coincident hyperspectral aircraft imagery and dense coincident surface observations

Science.gov (United States)

We analyzed 10 established and 4 new satellite reflectance algorithms for estimating chlorophyll-a (Chl-a) in a temperate reservoir in southwest Ohio using coincident hyperspectral aircraft imagery and dense water truth collected within one hour of image acquisition to develop si...

Genetic Analysis and Molecular Mapping of a Novel Chlorophyll-Deficit Mutant Gene in Rice

Directory of Open Access Journals (Sweden)

Xiao-qun HUANG

2008-03-01

Full Text Available A rice etiolation mutant 824ys featured with chlorophyll deficiency was identified from a normal green rice variety 824B. It showed whole green-yellow plant from the seedling stage, reduced number of tillers and longer growth duration. The contents of chlorophyll, chlorophyll a, chlorophyll b and net photosynthetic rate in leaves of the mutant obviously decreased, as well as the number of spikelets per panicle, seed setting rate and 1000-grain weight compared with its wild-type parent. Genetic analyses on F1 and F2 generations of 824ys crossed with three normal green varieties showed that the chlorophyll-deficit mutant character was controlled by a pair of recessive nuclear gene. Genetic mapping of the mutant gene was conducted by using microsatellite markers and F2 mapping population of 495R/824ys, and the mutant gene of 824ys was mapped on the short arm of rice chromosome 3. The genetic distances from the target gene to the markers RM218, RM282 and RM6959 were 25.6 cM, 5.2 cM and 21.8 cM, respectively. It was considered to be a new chlorophyll-deficit mutant gene and tentatively named as chl11(t.

Spectral difference method applied in determination of beta-carotene, chlorophyll a and b from irradiated seeds of Calendula Officinalis

International Nuclear Information System (INIS)

Cucu, Delia-Irina; Dorobantu, Ioan

2002-01-01

Beta-carotene is part of the class of the carotenoid pigments that have a special function in the pigmentation of flowers and fruits. These pigments are essential in photosynthesis where they are retaining energy and are photoprotectors by forming the redox system. They are also precursors of vitamin A, pigments involved in the process of seeing. Beta-carotene is responsible for the growth and development of organisms, for the protection and recovery of epithelial tissue, etc. Next to chlorophyll, carotenoids are the major components of the complex pigment-protein from the thylacoide membrane, and some of them, such as beta-carotene, were for a long time considered to be protecting the membrane against destructive events caused by the over excitement of the chlorophyll. This paper presents spectral difference method for physico-chemical characterisation of three components (beta-carotene, chlorophyll a and b) from Calendula officinalis leaves and stalks, after irradiation of germinated seeds. The germinated seeds of Calendula officinalis has been irradiated at 8 different doses ( 1 k Rad, 3 k Rad, 5 k Rad, 10 k Rad, 20 k Rad, 50 k Rad, 100 k Rad, 500 krad), one set of seeds remaining unirradiated for comparison. The plants had been grown in the same conditions of light, temperature and humidity. The identification of beta-carotene was performed through thin layer chromatography. The chemical concentrations of beta-carotene, chlorophyll a and b were determined by spectral difference method. (authors)

Concentration and saturation effects of tethered polymer chains on adsorbing surfaces

Science.gov (United States)

Descas, Radu; Sommer, Jens-Uwe; Blumen, Alexander

2006-12-01

We consider end-grafted chains at an adsorbing surface under good solvent conditions using Monte Carlo simulations and scaling arguments. Grafting of chains allows us to fix the surface concentration and to study a wide range of surface concentrations from the undersaturated state of the surface up to the brushlike regime. The average extension of single chains in the direction parallel and perpendicular to the surface is analyzed using scaling arguments for the two-dimensional semidilute surface state according to Bouchaud and Daoud [J. Phys. (Paris) 48, 1991 (1987)]. We find good agreement with the scaling predictions for the scaling in the direction parallel to the surface and for surface concentrations much below the saturation concentration (dense packing of adsorption blobs). Increasing the grafting density we study the saturation effects and the oversaturation of the adsorption layer. In order to account for the effect of excluded volume on the adsorption free energy we introduce a new scaling variable related with the saturation concentration of the adsorption layer (saturation scaling). We show that the decrease of the single chain order parameter (the fraction of adsorbed monomers on the surface) with increasing concentration, being constant in the ideal semidilute surface state, is properly described by saturation scaling only. Furthermore, the simulation results for the chains' extension from higher surface concentrations up to the oversaturated state support the new scaling approach. The oversaturated state can be understood using a geometrical model which assumes a brushlike layer on top of a saturated adsorption layer. We provide evidence that adsorbed polymer layers are very sensitive to saturation effects, which start to influence the semidilute surface scaling even much below the saturation threshold.

Potential controls of isoprene in the surface ocean

Science.gov (United States)

Hackenberg, S. C.; Andrews, S. J.; Airs, R.; Arnold, S. R.; Bouman, H. A.; Brewin, R. J. W.; Chance, R. J.; Cummings, D.; Dall'Olmo, G.; Lewis, A. C.; Minaeian, J. K.; Reifel, K. M.; Small, A.; Tarran, G. A.; Tilstone, G. H.; Carpenter, L. J.

2017-04-01

Isoprene surface ocean concentrations and vertical distribution, atmospheric mixing ratios, and calculated sea-to-air fluxes spanning approximately 125° of latitude (80°N-45°S) over the Arctic and Atlantic Oceans are reported. Oceanic isoprene concentrations were associated with a number of concurrently monitored biological variables including chlorophyll a (Chl a), photoprotective pigments, integrated primary production (intPP), and cyanobacterial cell counts, with higher isoprene concentrations relative to all respective variables found at sea surface temperatures greater than 20°C. The correlation between isoprene and the sum of photoprotective carotenoids, which is reported here for the first time, was the most consistent across all cruises. Parameterizations based on linear regression analyses of these relationships perform well for Arctic and Atlantic data, producing a better fit to observations than an existing Chl a-based parameterization. Global extrapolation of isoprene surface water concentrations using satellite-derived Chl a and intPP reproduced general trends in the in situ data and absolute values within a factor of 2 between 60% and 85%, depending on the data set and algorithm used.

Chlorophyll a, temperature, salinity and other variables collected from surface underway observations using flow-through pump from NOAA Ship Gordon Gunter off the U.S. East Coast during the East Coast Ocean Acidification (ECOA) Cruise from 2015-06-19 to 2015-07-24 (NCEI Accession 0157812)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This archival package contains chlorophyll a, temperature, salinity and other variables collected from surface underway observations during the East Coast Ocean...

Chlorophyll Synthase under Epigenetic Surveillance Is Critical for Vitamin E Synthesis, and Altered Expression Affects Tocopherol Levels in Arabidopsis1[OPEN

Science.gov (United States)

Zhang, Chunyu; Zhang, Wei; Ren, Guodong; Li, Delin; Cahoon, Rebecca E.; Chen, Ming; Zhou, Yongming; Yu, Bin

2015-01-01

Chlorophyll synthase catalyzes the final step in chlorophyll biosynthesis: the esterification of chlorophyllide with either geranylgeranyl diphosphate or phytyl diphosphate (PDP). Recent studies have pointed to the involvement of chlorophyll-linked reduction of geranylgeranyl by geranylgeranyl reductase as a major pathway for the synthesis of the PDP precursor of tocopherols. This indirect pathway of PDP synthesis suggests a key role of chlorophyll synthase in tocopherol production to generate the geranylgeranyl-chlorophyll substrate for geranylgeranyl reductase. In this study, contributions of chlorophyll synthase to tocopherol formation in Arabidopsis (Arabidopsis thaliana) were explored by disrupting and altering expression of the corresponding gene CHLOROPHYLL SYNTHASE (CHLSYN; At3g51820). Leaves from the homozygous chlysyn1-1 null mutant were nearly devoid of tocopherols, whereas seeds contained only approximately 25% of wild-type tocopherol levels. Leaves of RNA interference lines with partial suppression of CHLSYN displayed marked reductions in chlorophyll but up to a 2-fold increase in tocopherol concentrations. Cauliflower mosaic virus35S-mediated overexpression of CHLSYN unexpectedly caused a cosuppression phenotype at high frequencies accompanied by strongly reduced chlorophyll content and increased tocopherol levels. This phenotype and the associated detection of CHLSYN-derived small interfering RNAs were reversed with CHLSYN overexpression in rna-directed rna polymerase6 (rdr6), which is defective in RNA-dependent RNA polymerase6, a key enzyme in sense transgene-induced small interfering RNA production. CHLSYN overexpression in rdr6 had little effect on chlorophyll content but resulted in up to a 30% reduction in tocopherol levels in leaves. These findings show that altered CHLSYN expression impacts tocopherol levels and also, show a strong epigenetic surveillance of CHLSYN to control chlorophyll and tocopherol synthesis. PMID:26048882

The spontaneous chlorophyll mutation frequency in barley

DEFF Research Database (Denmark)

Jørgensen, Jørgen Helms; Jensen, Hans Peter

1986-01-01

A total of 1866 barley plants were progeny tested in the greenhouse. Twenty-five plants segregated for newly arisen, spontaneous chlorophyll mutant genes. Among the total of 470,129 seedlings screened there were 79 mutants (1.7 .+-. 0.6 .times. 10-4). The data are added to data from three similar...... materials and the resulting estimate of the chlorophyll mutant frequency is 1.6 .times. 10-4 in about 1.43 million seedlings. The estimate of the chlorophyll mutation rate per generation is close to 67.3 .times. 10-4 per diploid genome or in the order of 6 .times. 10-7 per locus and haploid genome....

Enhanced chlorophyll a and primary production in the northern Arabian Sea during the spring intermonsoon due to green Noctiluca scintillans bloom

Digital Repository Service at National Institute of Oceanography (India)

Madhu, N.V.; Jyothibabu, R.; Maheswaran, P.A.; Jayaraj, K.A.; Achuthankutty, C.T.

The surface waters of the northeastern Arabian Sea sustained relatively high chlorophyll a (average 0.81+ or -0.80 mg m sup(-3)) and primary production (average 29.5+ or -23.6 mgC m sup(-3)d sup(-1)) during the early spring intermonsoon 2000...

[Estimation of forest canopy chlorophyll content based on PROSPECT and SAIL models].

Science.gov (United States)

Yang, Xi-guang; Fan, Wen-yi; Yu, Ying

2010-11-01

The forest canopy chlorophyll content directly reflects the health and stress of forest. The accurate estimation of the forest canopy chlorophyll content is a significant foundation for researching forest ecosystem cycle models. In the present paper, the inversion of the forest canopy chlorophyll content was based on PROSPECT and SAIL models from the physical mechanism angle. First, leaf spectrum and canopy spectrum were simulated by PROSPECT and SAIL models respectively. And leaf chlorophyll content look-up-table was established for leaf chlorophyll content retrieval. Then leaf chlorophyll content was converted into canopy chlorophyll content by Leaf Area Index (LAD). Finally, canopy chlorophyll content was estimated from Hyperion image. The results indicated that the main effect bands of chlorophyll content were 400-900 nm, the simulation of leaf and canopy spectrum by PROSPECT and SAIL models fit better with the measured spectrum with 7.06% and 16.49% relative error respectively, the RMSE of LAI inversion was 0. 542 6 and the forest canopy chlorophyll content was estimated better by PROSPECT and SAIL models with precision = 77.02%.

DAYTIME VARIATIONS OF CHLOROPHYLL A FLUORESCENCE IN PAU D'ALHO SEEDLINGS

OpenAIRE

Bacarin, Marcos Antonio; Martinazzo, Emanuela Garbin; Cassol, Daniela; Falqueto, Antelmo Ralph; Silva, Diolina Moura

2016-01-01

ABSTRACT Analysis of transient and modulated fluorescence of chlorophyll a were made at one-hour intervals during an eight-hour period starting at 07:30h aiming to study mechanisms of photoprotection against high radiation and temperature in Gallesia integrifolia plants. Seeds were germinated inside plastic pots containing soil as substrate. At 120 days after emergence, chlorophyll fluorescence measurements were performed using Handy-PEA and FMS2 fluorometers. During the course of a day, an i...

Remote measurement of water color in coastal waters. [spectral radiance data used to obtain quantitative values for chlorophyll and turbidity

Science.gov (United States)

Weldon, J. W.

1973-01-01

An investigation was conducted to develop procedure to obtain quantitative values for chlorophyll and turbidity in coastal waters by observing the changes in spectral radiance of the backscattered spectrum. The technique under consideration consists of Examining Exotech model 20-D spectral radiometer data and determining which radiance ratios best correlated with chlorophyll and turbidity measurements as obtained from analyses of water samples and sechi visibility readings. Preliminary results indicate that there is a correlation between backscattered light and chlorophyll concentration and secchi visibility. The tests were conducted with the spectrometer mounted in a light aircraft over the Mississippi Sound at altitudes of 2.5K, 2.8K and 10K feet.

Variations of surface ozone concentration across the Klang Valley, Malaysia

Science.gov (United States)

Latif, Mohd Talib; Huey, Lim Shun; Juneng, Liew

2012-12-01

Hourly air quality data covering the period 2004-2008 was obtained from the Air Quality Division, the Department of Environment (DOE) through long-term monitoring by Alam Sekitar Sdn. Bhd. (ASMA) were analysed to investigate the variations of surface ozone (O3) in the Klang Valley, Malaysia. A total of nine monitoring stations were selected for analysis in this study and the results show that there are distinct seasonal patterns in the surface O3 across the Klang Valley. A high surface O3 concentration is usually observed between January and April, while a low surface O3 concentration is found between June and August. Analysis of daily variations in surface O3 and the precursors - NO, NO2, CO, NMHC and UVb, indicate that the surface O3 photochemistry in this study area exhibits a positive response to the intensity and wavelength in UVb while being influenced by the concentration of NOx, particularly through tritration processes. Although results from our study suggested that NMHCs may influence the maximum O3 concentration, further investigation is required. Wind direction during different monsoons was found to influence the concentration of O3 around the Klang Valley. HYSPLIT back trajectories (-72 h) were used to indicate the air-mass transport patterns on days with high concentrations of surface O3 in the study area. Results show that 47% of the high O3 days was associated with the localized circulation. The remaining 32% and 22% were associated with mid-range and long-range transport across the South China Sea from the northeast.