WorldWideScience

Sample records for super high multiplex

  1. Super-multiplex vibrational imaging

    Science.gov (United States)

    Wei, Lu; Chen, Zhixing; Shi, Lixue; Long, Rong; Anzalone, Andrew V.; Zhang, Luyuan; Hu, Fanghao; Yuste, Rafael; Cornish, Virginia W.; Min, Wei

    2017-04-01

    potential of this 24-colour (super-multiplex) optical imaging approach for elucidating intricate interactions in complex biological systems.

  2. New Compensation Technique for Fiber Impairments in Ultra High Capacity Super channel Based on (QDPASK Dense wavelength division multiplex (DWDM systems

    Directory of Open Access Journals (Sweden)

    Ibrahim A.Murdas

    2017-07-01

    Full Text Available In this paper we want to increase the data rate and to get larger transmission distance, therefore we use the Advanced modulation technique QDPASK in DWDM system with low channels space. In case of long transmission distance and multi channels system both giving rise to inter channel crosstalk induced by fiber nonlinearities as a result the use of advanced modulation technique becomes difficult. In this paper Quaternary differential phase amplitude shift keying (QDPASK is designed for 32 dense wavelength division multiplexing (DWDM channels, therefore we proposed a new cascade compensation system consist of optical and digital back propagation techniques (ODBP for compensate the linear and nonlinear effects . A performance of the system was reported using QDPASK DWDM fiber-optic system for various system parameters. The research is tested in VPI maker environment.

  3. Super Resolution from Hyperview Image Stack by Spatial Multiplexing

    Science.gov (United States)

    Grasnick, Armin

    2016-09-01

    An image stack for a hyperview representation could contain millions of different perspective views with extreme image similarity. The recording of all views from a computational 3d model implicates a lateral displacement of the virtual camera. Because of the huge number of views, the offset in between two adjoining camera positions can be very minor. If such a virtual setup reproduces a real hyperview screen setup, the offset can be below the wavelength of the visible light. But even with such small changes, there is an intrinsic probability for a measurable difference in between two neighbour images. Such image dissimilarity can be proofed successfully also in very basic 3d scenes. By using a quantity of juxtapositional images from the hyperview image stack, the resolution of the rendered images can be considerably improved, which is commonly known as super resolution. The utilisation of super resolution images in hyperview could cut the necessity of full frame computing and will reduce the effective render time.

  4. Super-channel oriented routing, spectrum and core assignment under crosstalk limit in spatial division multiplexing elastic optical networks

    Science.gov (United States)

    Zhao, Yongli; Zhu, Ye; Wang, Chunhui; Yu, Xiaosong; Liu, Chuan; Liu, Binglin; Zhang, Jie

    2017-07-01

    With the capacity increasing in optical networks enabled by spatial division multiplexing (SDM) technology, spatial division multiplexing elastic optical networks (SDM-EONs) attract much attention from both academic and industry. Super-channel is an important type of service provisioning in SDM-EONs. This paper focuses on the issue of super-channel construction in SDM-EONs. Mixed super-channel oriented routing, spectrum and core assignment (MS-RSCA) algorithm is proposed in SDM-EONs considering inter-core crosstalk. Simulation results show that MS-RSCA can improve spectrum resource utilization and reduce blocking probability significantly compared with the baseline RSCA algorithms.

  5. Single-exposure super-resolved interferometric microscopy by red-green-blue multiplexing.

    Science.gov (United States)

    Calabuig, Alejandro; Micó, Vicente; Garcia, Javier; Zalevsky, Zeev; Ferreira, Carlos

    2011-03-15

    We present single-exposure super-resolved interferometric microscopy (SESRIM) as a novel approach capable of providing one-dimensional (1-D) super-resolution (SR) imaging in holographic microscopy using a single illumination shot. The single-exposure SR working principle is achieved by combining angular and wavelength multiplexing incoming from a set of tilted beams with different wavelengths where each wavelength is tuned with the red-green-blue (RGB) channels of a color CCD. Thus, the information included in each color channel is retrieved by holographic recording using a single-color CCD capture and by analyzing the RGB channels. Finally, 1-D SR imaging is obtained after the digital postprocessing stage yielding the generation of a synthetic aperture. Experimental results are reported validating the proposed SESRIM approach while an extension of the proposed approach to the two-dimensional case is considered.

  6. Passive Standoff Super Resolution Imaging using Spatial-Spectral Multiplexing

    Science.gov (United States)

    2017-08-14

    with a Fourier transform spectrometer, requiring the FTAS system to operate in the near - infrared , which need only effect the coatings and FPE...A. Cook, and J. Hair, “ System analysis of a tilted field-widened Michelson interferometer for high spectral resolution lidar ”, Opt. Expr. Vol. 20, No...General Fabry-Perot System ................................................ 48 2.6 Tolerancing and Aberrations

  7. Time multiplexing super resolution using a 2D Barker-based array

    Science.gov (United States)

    Ilovitsh, Asaf; Ilovitsh, Tali; Preter, Eyal; Levanon, Nadav; Zalevsky, Zeev

    2016-03-01

    We propose the use of a two dimensional Barker-based array in order to improve the performance of the standard time multiplexing super resolution system. The Barker-based array is a 2D generalization of the standard 1D Barker code. It enables achieving a two dimensional super resolution image using only one dimensional scan, by exploiting its unique auto correlation property. A sequence of low resolution images are captured at different lateral positions of the array, and are decoded properly using the same array. In addition, we present the use of a mismatched array for the decoding process. The cross correlation between the Barker-based array and the mismatched array has a perfect peak to sidelobes ratio, making it ideal for the super resolution process. Also, we propose the projection of this array onto the object using a phase-only spatial light modulator. Projecting the array eliminates the need for printing it, mechanically shifting it, and having a direct contact with the object, which is not feasible in many imaging applications. The proposed method is presented analytically, demonstrated via numerical simulation, and validated by laboratory experiments.

  8. Single-exposure super-resolved interferometric microscopy by RGB multiplexing in lensless configuration

    Science.gov (United States)

    Granero, Luis; Ferreira, Carlos; Zalevsky, Zeev; García, Javier; Micó, Vicente

    2016-07-01

    Single-Exposure Super-Resolved Interferometric Microscopy (SESRIM) reports on a way to achieve one-dimensional (1-D) superresolved imaging in digital holographic microscopy (DHM) by a single illumination shot and digital recording. SESRIM provides color-coded angular multiplexing of the accessible sample's range of spatial frequencies and it allows their recording in a single CCD (color or monochrome) snapshot by adding 3 RGB coherent reference beams at the output plane. In this manuscript, we extend the applicability of SESRIM to the field of digital in-line holographic microscopy (DIHM), that is, working without lenses. As consequence of the in-line configuration, an additional restriction concerning the object field of view (FOV) must be imposed to the technique. Experimental results are reported for both a synthetic object (USAF resolution test target) and a biological sample (swine sperm sample) validating this new kind of superresolution imaging method named as lensless SESRIM (L-SESRIM).

  9. High Channel Count, Low Cost, Multiplexed FBG Sensor Systems

    Institute of Scientific and Technical Information of China (English)

    J. J. Pan; FengQing Zhou; Kejian Guan; Joy Jiang; Liang Dong; Albert Li; Xiangdong Qiu; Jonathan Zhang

    2003-01-01

    With rich products development experience in WDM telecommunication networks, we introduce a few of high channel count, multiplexed FBG fiber optic sensor systems featured in reliable high performance and low cost.

  10. Depth profilometry via multiplexed optical high-coherence interferometry

    National Research Council Canada - National Science Library

    Kazemzadeh, Farnoud; Wong, Alexander; Behr, Bradford B; Hajian, Arsen R

    2015-01-01

    ... such as defect detection, corrosion assessment, and dental assessment to name a few. In this study, we investigate the feasibility of depth profilometry using an Multiplexed Optical High-coherence Interferometry MOHI instrument...

  11. Characteristics of Multiplexed Grooved Nozzles for High Flow Rate Electrospray

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kyoung Tae; Kim, Woo Jin; Kim, Sang Soo [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of)

    2007-10-15

    The electrospray operated in the cone-jet mode can generate highly charged micro droplets in an almost uniform size at flow rates. Therefore, the multiplexing system which can retain the characteristics of the cone-jet mode is inevitable for the electrospray application. This experiment reports the multiplexed grooved nozzle system with the extractor. The effects of the grooves and the extractor on the performance of the electrospray were evaluated through experiments. Using the grooved nozzle, the stable cone-jet mode can be achieved at the each groove in the grooved mode. Furthermore, the number of nozzles per unit area is increased by the extractor. The multiplexing density is 12 jets per cm{sup 2} at 30 mm distance from the nozzle tip to the ground plate. The multiplexing system for the high flow rate electrospray is realized with the extractor which can diminish the space charge effect without sacrificing characteristics of the cone-jet mode.

  12. 76 FR 48169 - Advancing Regulatory Science for Highly Multiplexed Microbiology/Medical Countermeasure Devices...

    Science.gov (United States)

    2011-08-08

    ... following public meeting: ``Advancing Regulatory Science for Highly Multiplexed Microbiology/Medical... microbiology/MCM devices. The ultimate goal is to advance regulatory science for highly multiplexed devices... HUMAN SERVICES Food and Drug Administration Advancing Regulatory Science for Highly...

  13. 76 FR 71982 - Advancing Regulatory Science for Highly Multiplexed Microbiology/Medical Countermeasure Devices...

    Science.gov (United States)

    2011-11-21

    ... paper entitled ''Advancing Regulatory Science for Highly Multiplexed Microbiology/Medical Countermeasure... the ``Advancing Regulatory Science for Highly Multiplexed Microbiology/Medical Countermeasure Devices... HUMAN SERVICES Food and Drug Administration Advancing Regulatory Science for Highly...

  14. High resolution multiplexed functional imaging in live embryos (Conference Presentation)

    Science.gov (United States)

    Xu, Dongli; Zhou, Weibin; Peng, Leilei

    2017-02-01

    Fourier multiplexed fluorescence lifetime imaging (FmFLIM) scanning laser optical tomography (FmFLIM-SLOT) combines FmFLIM and Scanning laser optical tomography (SLOT) to perform multiplexed 3D FLIM imaging of live embryos. The system had demonstrate multiplexed functional imaging of zebrafish embryos genetically express Foster Resonant Energy Transfer (FRET) sensors. However, previous system has a 20 micron resolution because the focused Gaussian beam diverges quickly from the focused plane, makes it difficult to achieve high resolution imaging over a long projection depth. Here, we present a high-resolution FmFLIM-SLOT system with achromatic Bessel beam, which achieves 3 micron resolution in 3D deep tissue imaging. In Bessel-FmFLIM-SLOT, multiple laser excitation lines are firstly intensity modulated by a Michelson interferometer with a spinning polygon mirror optical delay line, which enables Fourier multiplexed multi-channel lifetime measurements. Then, a spatial light modulator and a prism are used to transform the modulated Gaussian laser beam to an achromatic Bessel beam. The achromatic Bessel beam scans across the whole specimen with equal angular intervals as sample rotated. After tomography reconstruction and the frequency domain lifetime analysis method, both the 3D intensity and lifetime image of multiple excitation-emission can be obtained. Using Bessel-FmFLIM-SLOT system, we performed cellular-resolution FLIM tomography imaging of live zebrafish embryo. Genetically expressed FRET sensors in these embryo will allow non-invasive observation of multiple biochemical processes in vivo.

  15. Method and apparatus for connecting high voltage leads to a high temperature super-conducting transformer

    Science.gov (United States)

    Golner, Thomas M.; Mehta, Shirish P.

    2005-07-26

    A method and apparatus for connecting high voltage leads to a super-conducting transformer is provided that includes a first super-conducting coil set, a second super-conducting coil set, and a third super-conducting coil set. The first, second and third super-conducting coil sets are connected via an insulated interconnect system that includes insulated conductors and insulated connectors that are utilized to connect the first, second, and third super-conducting coil sets to the high voltage leads.

  16. A high-throughput multiplex method adapted for GMO detection.

    Science.gov (United States)

    Chaouachi, Maher; Chupeau, Gaëlle; Berard, Aurélie; McKhann, Heather; Romaniuk, Marcel; Giancola, Sandra; Laval, Valérie; Bertheau, Yves; Brunel, Dominique

    2008-12-24

    A high-throughput multiplex assay for the detection of genetically modified organisms (GMO) was developed on the basis of the existing SNPlex method designed for SNP genotyping. This SNPlex assay allows the simultaneous detection of up to 48 short DNA sequences (approximately 70 bp; "signature sequences") from taxa endogenous reference genes, from GMO constructions, screening targets, construct-specific, and event-specific targets, and finally from donor organisms. This assay avoids certain shortcomings of multiplex PCR-based methods already in widespread use for GMO detection. The assay demonstrated high specificity and sensitivity. The results suggest that this assay is reliable, flexible, and cost- and time-effective for high-throughput GMO detection.

  17. Highly Multiplexed Profiling of Low Abundance Tumor Mutations in Plasma

    Science.gov (United States)

    Wiggin, Matthew; Pel, Joel; Vysotskaia, Valentina; Broemeling, David; Marziali, Andre; Hanson, Dan

    2013-01-01

    We have demonstrated a novel somatic mutation enrichment methodology demonstrating multiplexed detection of tumor mutations in plasma with sensitivity as low as 0.01% compared to normal DNA. This highly sensitive detection of low abundance mutations is achieved using electrophoretic separation and enrichment of DNA fragments containing point mutations over their wild-type counterparts. Commercialized as the OnTarget platform by Boreal Genomics, the system enriches nucleic acid samples for specific targets prior to amplification and detection, enabling the use of next-generation sequencing (NGS) or other detection assays for plasma or FFPE-based mutation detection and profiling. We present data demonstrating highly sensitive and multiplexed detection of panels of up to 100 mutations in plasma samples, improving the sensitivity of NGS assays to below 0.01% mutant content. We also report on concordance studies comparing low tumor content FFPE tissue and matched plasma in human samples demonstrating that OnTarget represents a robust, highly sensitive and multiplexed platform for non-invasive tumor monitoring.

  18. What is so super about super-emitters? Characterizing methane high emitters from natural gas infrastructure

    Science.gov (United States)

    Zavala Araiza, D.; Lyon, D. R.; Alvarez, R.; Harriss, R. C.; Palacios, V.; Hamburg, S.

    2015-12-01

    Methane emissions across the natural gas supply chain are dominated at any one time by a few high-emitters (super-emitters or fat-tail of the distribution), often underrepresented in published datasets used to construct emission inventories. Characterization of high-emitters is essential for improving emission estimates based on atmospheric data (top-down) and emission inventories (bottom-up). The population of high-emitters (e.g. 10-20% of sites that account for 80-90% of the emissions) is temporally and spatially dynamic. As a consequence, it is challenging to design sampling methods and construct estimates that accurately represent their frequency and magnitude of emissions. We present new methods to derive facility-specific emission distribution functions that explicitly integrate the influence of the relatively rare super-emitters. These methods were applied in the Barnett Shale region to construct a custom emission inventory that is then compared to top-down emission estimates for the region. We offer a methodological framework relevant to the design of future sampling campaigns, in which these high-emitters are seamlessly incorporated to representative emissions distributions. This framework can be applied to heterogeneous oil and gas production regions across geographies to obtain accurate regional emission estimates. Additionally, we characterize emissions relative to the fraction of a facility's total methane throughput; an effective metric to identify sites with excess emissions resulting from avoidable operating conditions, such as malfunctioning equipment (defined here as functional super-emitters). This work suggests that identifying functional super-emitters and correcting their avoidable operating conditions would result in significant emission reductions. However, due to their spatiotemporal dynamic behavior, achieving and maintaining uniformly low emissions across the entire population of sites will require mitigation steps (e.g. leak detection

  19. High density 3D printed microfluidic valves, pumps, and multiplexers.

    Science.gov (United States)

    Gong, Hua; Woolley, Adam T; Nordin, Gregory P

    2016-07-07

    In this paper we demonstrate that 3D printing with a digital light processor stereolithographic (DLP-SLA) 3D printer can be used to create high density microfluidic devices with active components such as valves and pumps. Leveraging our previous work on optical formulation of inexpensive resins (RSC Adv., 2015, 5, 106621), we demonstrate valves with only 10% of the volume of our original 3D printed valves (Biomicrofluidics, 2015, 9, 016501), which were already the smallest that have been reported. Moreover, we show that incorporation of a thermal initiator in the resin formulation along with a post-print bake can dramatically improve the durability of 3D printed valves up to 1 million actuations. Using two valves and a valve-like displacement chamber (DC), we also create compact 3D printed pumps. With 5-phase actuation and a 15 ms phase interval, we obtain pump flow rates as high as 40 μL min(-1). We also characterize maximum pump back pressure (i.e., maximum pressure the pump can work against), maximum flow rate (flow rate when there is zero back pressure), and flow rate as a function of the height of the pump outlet. We further demonstrate combining 5 valves and one DC to create a 3-to-2 multiplexer with integrated pump. In addition to serial multiplexing, we also show that the device can operate as a mixer. Importantly, we illustrate the rapid fabrication and test cycles that 3D printing makes possible by implementing a new multiplexer design to improve mixing, and fabricate and test it within one day.

  20. Subtyping Animal Influenza Virus with General Multiplex RT-PCR and Liquichip High Throughput (GMPLex)

    Institute of Scientific and Technical Information of China (English)

    Zhi-feng Qin; Bing Cheng; Zhou-xi Ruan; Ying-zuo Bi; Joseph J Giambrone; Hong-zhuan Wu; Jie Sun; Ti-kang Lu; Shao-ling Zeng; Qun-yi Hua; Qing-yan Ling; Shu-kun Chen; Jian-qiang Lv; Cai-hong Zhang

    2012-01-01

    This study developed a multiplex RT-PCR integrated with luminex technology to rapidly subtype simultaneously multiple influenza viruses.Primers and probes were designed to amplify NS and M genes of influenza A viruses HA gene of H1,H3,H5,H7,H9 subtypes,and NA gene of the N1 and N2 subtypes.Universal super primers were introduced to establish a multiplex RT-PCR (GM RT-PCR).It included three stages of RT-PCR amplification,and then the RT-PCR products were further tested by LiquiChip probe,combined to give an influenza virus (Ⅳ) rapid high throughput subtyping test,designated as GMPLex.The IV GMPLex rapid high throughput subtyping test presents the following features:high throughput,able to determine the subtypes of 9 target genes in H1,H3,H5,H7,H9,N1,and N2 subtypes of the influenza A virus at one time; rapid,completing the influenza subtyping within 6 hours; high specificity,ensured the specificity of the different subtypes by using two nested degenerate primers and one probe,no cross reaction occurring between the subtypes,no non-specific reactions with other pathogens and high sensitivity.When used separately to detect the product of single GM RT-PCR for single H5 or N1 gene,the GMPLex test showed a sensitivity of 105(=280ELD50) forboth tests and the Luminex qualitative ratio results were 3.08 and 3.12,respectively.When used to detect the product of GM RT-PCR for HSN1 strain at the same time,both showed a sensitivity of 10-4(=2800 ELD50).The GMPLex rapid high throughput subtyping test can satisfy the needs of influenza rapid testing.

  1. Multiplexed labeling system for high-throughput cell sorting.

    Science.gov (United States)

    Shin, Seung Won; Park, Kyung Soo; Song, In Hyun; Shin, Woo Jung; Kim, Byung Woo; Kim, Dong-Ik; Um, Soong Ho

    2016-09-01

    Flow cytometry and fluorescence activated cell sorting techniques were designed to realize configurable classification and separation of target cells. A number of cell phenotypes with different functionalities have recently been revealed. Before simultaneous selective capture of cells, it is desirable to label different samples with the corresponding dyes in a multiplexing manner to allow for a single analysis. However, few methods to obtain multiple fluorescent colors for various cell types have been developed. Even when restricted laser sources are employed, a small number of color codes can be expressed simultaneously. In this study, we demonstrate the ability to manifest DNA nanostructure-based multifluorescent colors formed by a complex of dyes. Highly precise self-assembly of fluorescent dye-conjugated oligonucleotides gives anisotropic DNA nanostructures, Y- and tree-shaped DNA (Y-DNA and T-DNA, respectively), which may be used as platforms for fluorescent codes. As a proof of concept, we have demonstrated seven different fluorescent codes with only two different fluorescent dyes using T-DNA. This method provides maximum efficiency for current flow cytometry. We are confident that this system will provide highly efficient multiplexed fluorescent detection for bioanalysis compared with one-to-one fluorescent correspondence for specific marker detection.

  2. Two-Dimensional Super High Density Multi-Fiber Connector

    Institute of Scientific and Technical Information of China (English)

    Takashi Shigenaga; Katsuki Suematsu; Masao Shinoda; Takayuki Ando

    2003-01-01

    We have developed 32-fiber and 60-fiber super high density multi fiber connector. This 32-fiber connector can be applicable for single-mode fiber and 60-fiber connector for multi-mode fiber. We have also established PC (physical contact) connection technology by optimizing polishing condition and clamping force.

  3. Depth profilometry via multiplexed optical high-coherence interferometry.

    Directory of Open Access Journals (Sweden)

    Farnoud Kazemzadeh

    Full Text Available Depth Profilometry involves the measurement of the depth profile of objects, and has significant potential for various industrial applications that benefit from non-destructive sub-surface profiling such as defect detection, corrosion assessment, and dental assessment to name a few. In this study, we investigate the feasibility of depth profilometry using an Multiplexed Optical High-coherence Interferometry MOHI instrument. The MOHI instrument utilizes the spatial coherence of a laser and the interferometric properties of light to probe the reflectivity as a function of depth of a sample. The axial and lateral resolutions, as well as imaging depth, are decoupled in the MOHI instrument. The MOHI instrument is capable of multiplexing interferometric measurements into 480 one-dimensional interferograms at a location on the sample and is built with axial and lateral resolutions of 40 μm at a maximum imaging depth of 700 μm. Preliminary results, where a piece of sand-blasted aluminum, an NBK7 glass piece, and an optical phantom were successfully probed using the MOHI instrument to produce depth profiles, demonstrate the feasibility of such an instrument for performing depth profilometry.

  4. Multiplexed CV quantum teleportation for high rates in quantum communication

    CERN Document Server

    Christ, Andreas; Silberhorn, Christine

    2012-01-01

    A major challenge of today's quantum communication systems lies in the transmission of quantum information with high rates over long distances in the presence of unavoidable losses. Thereby the achievable quantum communication rate is fundamentally limited by the amount of energy that can be transmitted per use of the channel. It is hence vital to develop quantum communication protocols which encode quantum information as energy efficiently as possible. To this aim we investigate continuous-variable quantum teleportation as a method of distributing quantum information. We explore the possibility to encode information on multiple optical modes and derive upper and lower bounds on the achievable quantum channel capacities. This analysis enables us to benchmark single-mode vs. multi-mode entanglement resources. Our research reveals that multiplexing does not only feature an enhanced energy efficiency, significantly increasing the achievable quantum communication rates in comparison to single-mode coding, but als...

  5. Super-SERS-active and highly effective antimicrobial Ag nanodendrites

    Science.gov (United States)

    Li, H. B.; Liu, P.; Liang, Y.; Xiao, J.; Yang, G. W.

    2012-07-01

    We have developed simple and green electrochemistry to synthesize Ag nanostructures with high purity, good crystallinity and smooth surface for applications as super-SERS (surface-enhanced Raman scattering), SERS-active substrates and with highly effective antimicrobial activities. This synthesis takes place in a clean and slow reaction environment without any chemical additives, which ensures an ultrahigh active surface of the as-synthesized Ag nanostructures owing to their purity, good crystallinity and smooth morphology. Using this method, we synthesized nearly perfect Ag nanodendrites (NDs), which exhibit super-SERS sensitivity when they are used to detect the SERS spectra of rhodamine 6G at concentrations as low as 5 × 10-16 M, and have an ultrahigh electromagnetic (EM) enhancement factor of the order of 1013, breaking through the theoretical limit of EM enhancement. Meanwhile, the as-synthesized Ag NDs possess highly effective antimicrobial activities for Escherichia coli, Candida albicans and Staphylococcus aureus, which are over 10 times that of silver nanoparticles. Additionally, the basic physics and chemistry involved in the fabrication of Ag nanostructures are pursued. These investigations show that silver nanostructures with highly active surfaces can make the most of Ag nanostructures functioning as super-SERS-active substrates and multiple antibiotics.

  6. High-speed pulse-shape generator, pulse multiplexer

    Science.gov (United States)

    Burkhart, Scott C.

    2002-01-01

    The invention combines arbitrary amplitude high-speed pulses for precision pulse shaping for the National Ignition Facility (NIF). The circuitry combines arbitrary height pulses which are generated by replicating scaled versions of a trigger pulse and summing them delayed in time on a pulse line. The combined electrical pulses are connected to an electro-optic modulator which modulates a laser beam. The circuit can also be adapted to combine multiple channels of high speed data into a single train of electrical pulses which generates the optical pulses for very high speed optical communication. The invention has application in laser pulse shaping for inertial confinement fusion, in optical data links for computers, telecommunications, and in laser pulse shaping for atomic excitation studies. The invention can be used to effect at least a 10.times. increase in all fiber communication lines. It allows a greatly increased data transfer rate between high-performance computers. The invention is inexpensive enough to bring high-speed video and data services to homes through a super modem.

  7. SuperB A High-Luminosity Asymmetric $e^+ e^-$ Super Flavour Factory : Conceptual Design Report

    CERN Document Server

    Bona, M.; Grauges Pous, E.; Colangelo, P.; De Fazio, F.; Palano, A.; Manghisoni, M.; Re, V.; Traversi, G.; Eigen, G.; Venturini, M.; Soni, N.; Bruschi, M.; De Castro, S.; Faccioli, P.; Gabrieli, A.; Giacobbe, B.; Semprini Cesare, N.; Spighi, R.; Villa, M.; Zoccoli, A.; Hearty, C.; McKenna, J.; Soni, A.; Khan, A.; Barniakov, A.Y.; Barniakov, M.Y.; Blinov, V.E.; Druzhinin, V.P.; Golubev, V.B.; Kononov, S.A.; Koop, I.A.; Kravchenko, E.A.; Levichev, E.B.; Nikitin, S.A.; Onuchin, A.P.; Piminov, P.A.; Serednyakov, S.I.; Shatilov, D.N.; Skovpen, Y.I.; Solodov, E.A.; Cheng, C.H.; Echenard, B.; Fang, F.; Hitlin, D.J.; Porter, F.C.; Asner, D.M.; Pham, T.N.; Fleischer, R.; Giudice, G.F.; Hurth, T.; Mangano, M.; Mancinelli, G.; Meadows, B.T.; Schwartz, A.J.; Sokoloff, M.D.; Soffer, A.; Beard, C.D.; Haas, T.; Mankel, R.; Hiller, G.; Ball, P.; Pappagallo, M.; Pennington, M.R.; Gradl, W.; Playfer, S.; Abada, A.; Becirevic, D.; Descotes-Genon, S.; Pene, O.; Andreotti, D.; Andreotti, M.; Bettoni, D.; Bozzi, C.; Calabresi, R.; Cecchi, A.; Cibinetto, G.; Franchini, P.; Luppi, E.; Negrini, M.; Petrella, A.; Piemontese, L.; Prencipe, E.; Santoro, V.; Stancari, G.; Anulli, F.; Baldini-Ferroli, R.; Biagini, M.E.; Boscolo, M.; Calcaterra, A.; Drago, A.; Finocchiaro, G.; Guiducci, S.; Isidori, G.; Pacetti, S.; Patteri, P.; Peruzzi, I.M.; Piccolo, M.; Preger, M.A.; Raimondi, P.; Rama, M.; Vaccarezza, C.; Zallo, A.; Zobov, M.; De Sangro, R.; Buzzo, A.; Lo Vetere, M.; Macri, M.; Monge, M.R.; Passaggio, S.; Patrignani, C.; Robutti, E.; Tosi, S.; Matias, J.; Panduro Vazquez, W.; Borzumati, F.; Eyges, V.; Prell, S.A.; Pedlar, T.K.; Korpar, S.; Pestonik, R.; Staric, M.; Neubert, M.; Denig, A.G.; Nierste, U.; Agoh, T.; Ohmi, K.; Ohnishi, Y.; Fry, J.R.; Touramanis, C.; Wolski, A.; Golob, B.; Krizan, P.; Flaecher, H.; Bevan, A.J.; Di Lodovico, F.; George, K.A.; Barlow, R.; Lafferty, G.; Jawahery, A.; Roberts, D.A.; Simi, G.; Patel, P.M.; Robertson, S.H.; Lazzaro, A.; Palombo, F.; Kaidalov, A.; Buras, A.J.; Tarantino, C.; Buchalla, G.; Sanda, A.I.; D'Ambrosio, G.; Ricciardi, G.; Bigi, I.; Jessop, C.P.; Losecco, J.M.; Honscheid, K.; Arnaud, N.; Chehab, R.; Fedala, Y.; Polci, F.; Roudeau, P.; Sordini, V.; Soskov, V.; Stocchi, A.; Variola, A.; Vivoli, A.; Wormser, G.; Zomer, F.; Bertolin, A.; Brugnera, R.; Gagliardi, N.; Gaz, A.; Margoni, M.; Morandin, M.; Posocco, M.; Rotondo, M.; Simonetto, F.; Stroili, R.; Bonneaud, G.R.; Lombardo, V.; Calderini, G.; Ratti, L.; Speziali, V.; Biasini, M.; Covarelli, R.; Manoni, E.; Servoli, L.; Angelini, C.; Batignani, G.; Bettarini, S.; Bosi, F.; Carpinelli, M.; Cenci, R.; Cervelli, A.; Dell'Orso, M.; Forti, F.; Giannetti, P.; Giorgi, M.; Lusiani, A.; Marchiori, G.; Massa, M.; Mazur, M.A.; Morsani, F.; Neri, N.; Paoloni, E.; Raffaelli, F.; Rizzo, G.; Walsh, J.; Braun, V.; Lenz, A.; Adams, G.S.; Danko, I.Z.; Baracchini, E.; Bellini, F.; Cavoto, G.; D'Orazio, A.; Del Re, D.; Di Marco, E.; Faccini, R.; Ferrarotto, F.; Gaspero, Mario; Jackson, P.; Martinelli, G.; Mazzoni, M.A.; Morganti, Silvio; Piredda, G.; Renga, F.; Silvestrini, L.; Voena, C.; Catani, L.; Di Ciaccio, A.; Messi, R.; Santovetti, E.; Satta, A.; Ciuchini, M.; Lubicz, V.; Wilson, F.F.; Godang, R.; Chen, X.; Liu, H.; Park, W.; Purohit, M.; Trivedi, A.; White, R.M.; Wilson, J.R.; Allen, M.T.; Aston, D.; Bartoldus, R.; Brodsky, S.J.; Cai, Y.; Coleman, J.; Convery, M.R.; DeBarger, S.; Dingfelder, J.C.; Dubois-Felsmann, G.P.; Ecklund, S.; Fisher, A.S.; Haller, G.; Heifets, S.A.; Kaminski, J.; Kelsey, M.H.; Kocian, M.L.; Leith, D.W.G.S.; Li, N.; Luitz, S.; Luth, V.; MacFarlane, D.; Messner, R.; Muller, D.R.; Nosochkov, Y.; Novokhatski, A.; Pivi, M.; Ratcliff, B.N.; Roodman, A.; Schwiening, J.; Seeman, J.; Snyder, A.; Sullivan, M.; Va'Vra, J.; Wienands, U.; Wisniewski, W.; Stoeck, H.; Cheng, H.Y.; Li, H.N.; Keum, Y.Y.; Gronau, M.; Grossman, Y.; Bianchi, F.; Gamba, D.; Gambino, P.; Marchetto, F.; Menichetti, Ezio A.; Mussa, R.; Pelliccioni, M.; Dalla Betta, G.F.; Bomben, M.; Bosisio, L.; Cartaro, C.; Lanceri, L.; Vitale, L.; Azzolini, V.; Bernabeu, J.; Lopez-March, N.; Martinez-Vidal, F.; Milanes, D.A.; Oyanguren, A.; Paradisi, P.; Pich, A.; Sanchis-Lozano, M.A.; Kowalewski, Robert V.; Roney, J.M.; Back, J.J.; Gershon, T.J.; Harrison, P.F.; Latham, T.E.; Mohanty, G.B.; Petrov, A.A.; Pierini, M.; INFN

    2007-01-01

    The physics objectives of SuperB, an asymmetric electron-positron collider with a luminosity above 10^36/cm^2/s are described, together with the conceptual design of a novel low emittance design that achieves this performance with wallplug power comparable to that of the current B Factories, and an upgraded detector capable of doing the physics in the SuperB environment.

  8. High-Speed Optical Local Access Network System Using Bi-Directional Polarization Multiplexing

    Institute of Scientific and Technical Information of China (English)

    Mitsuru Miyauchi; Yanjun Sun

    2003-01-01

    A high-speed and economical optical local access network system is proposed where bi-directional polarization multiplexing is applied to a bi-directional transmission. Experimental results using a prototype system confirmlow optical loss and environmental stabilities.

  9. High-Speed Optical Local Access Network System Using Bi-Directional Polarization Multiplexing

    Institute of Scientific and Technical Information of China (English)

    Mitsuru; Miyauchi; Yanjun; Sun

    2003-01-01

    A high-speed and economical optical local access network system is proposed where bi-directional polarization multiplexing is applied to a bi-directional transmission. Experimental results using a prototype system confirm low optical loss and environmental stabilities.

  10. Depth Profilometry via Multiplexed Optical High-Coherence Interferometry: e0121066

    National Research Council Canada - National Science Library

    Farnoud Kazemzadeh; Alexander Wong; Bradford B Behr; Arsen R Hajian

    2015-01-01

    ... such as defect detection, corrosion assessment, and dental assessment to name a few. In this study, we investigate the feasibility of depth profilometry using an Multiplexed Optical High-coherence Interferometry MOHI instrument...

  11. Low insertion loss highly mode-selective spatial multiplexers using multi-plane light conversion

    Science.gov (United States)

    Morizur, Jean-François; Barré, Nicolas; Pinel, Olivier; Lenglé, Kevin; Garcia, Lionel; Jaffres, Lionel; Jian, Pu; Labroille, Guillaume

    2016-02-01

    Multi-Plane Light Conversion enables novel beam shaping devices, including spatial multiplexers. After a presentation of the achievable performances of these spatial multiplexers, which can combine 10 spatial modes with cross-talk below -22 dB and insertion loss below 4 dB, we review the performances of Multi-Plane Light Con-version in multiple application cases. These application cases include mode-multiplexed optical amplification, high-power beam shaping and combining and LAN fiber capacity upgrade.

  12. SuperB: a Linear High-Luminosity B Factory

    Energy Technology Data Exchange (ETDEWEB)

    Albert, J.; Bettarini, S.; Biagini, M.; Bonneaud, G.; Cai, Y.; Calderini, G.; Ciuchini, M.; Dubois-Felsmann, G.P.; Ecklund, S.; Forti, F.; Gershon, T.J.; Giorgi, M.A.; Hitlin, D.G.; Leith, D.W.G.S.; Lusiani, A.; MacFarlane, D.B.; Martinez-Vidal, F.; Neri, N.; Novokhatski, A.; Pierini, M.; Piredda, G.; /Caltech /Pisa U. /Pisa, Scuola Normale

    2006-02-08

    This paper is based on the outcome of the activity that has taken place during the recent workshop on ''SuperB in Italy'' held in Frascati on November 11-12, 2005. The workshop was opened by a theoretical introduction of Marco Ciuchini and was structured in two working groups. One focused on the machine and the other on the detector and experimental issues.. The present status on CP is mainly based on the results achieved by BABAR and Belle. Establishment of the indirect CP violation in B sector in 2001 and of the direct CP violation in 2004 thanks to the success of PEP-II and KEKB e{sup +}e{sup -} asymmetric B Factories operating at the center of mass energy corresponding to the mass of the {Upsilon}(4S ). With the two B Factories taking data, the Unitarity Triangle is now beginning to be over constrained by improving the measurements of the sides and now also of the angles {alpha}, and {gamma}. We are also in presence of the very intriguing results about the measurements of sin2{beta} in the time dependent analysis of decay channels via penguin loops, where b {yields} s{bar s}s and b {yields} s{bar d}d. {tau} physics, in particular LFV search, as well as charm and ISR physics are important parts of the scientific program of a SuperB Factory. The physics case together with possible scenarios for the high luminosity SuperB Factory based on the concepts of the Linear Collider and the related experimental issues are discussed.

  13. A comprehensive platform for highly multiplexed mammalian functional genetic screens

    Directory of Open Access Journals (Sweden)

    Cheung-Ong Kahlin

    2011-05-01

    Full Text Available Abstract Background Genome-wide screening in human and mouse cells using RNA interference and open reading frame over-expression libraries is rapidly becoming a viable experimental approach for many research labs. There are a variety of gene expression modulation libraries commercially available, however, detailed and validated protocols as well as the reagents necessary for deconvolving genome-scale gene screens using these libraries are lacking. As a solution, we designed a comprehensive platform for highly multiplexed functional genetic screens in human, mouse and yeast cells using popular, commercially available gene modulation libraries. The Gene Modulation Array Platform (GMAP is a single microarray-based detection solution for deconvolution of loss and gain-of-function pooled screens. Results Experiments with specially constructed lentiviral-based plasmid pools containing ~78,000 shRNAs demonstrated that the GMAP is capable of deconvolving genome-wide shRNA "dropout" screens. Further experiments with a larger, ~90,000 shRNA pool demonstrate that equivalent results are obtained from plasmid pools and from genomic DNA derived from lentivirus infected cells. Parallel testing of large shRNA pools using GMAP and next-generation sequencing methods revealed that the two methods provide valid and complementary approaches to deconvolution of genome-wide shRNA screens. Additional experiments demonstrated that GMAP is equivalent to similar microarray-based products when used for deconvolution of open reading frame over-expression screens. Conclusion Herein, we demonstrate four major applications for the GMAP resource, including deconvolution of pooled RNAi screens in cells with at least 90,000 distinct shRNAs. We also provide detailed methodologies for pooled shRNA screen readout using GMAP and compare next-generation sequencing to GMAP (i.e. microarray based deconvolution methods.

  14. Ultra High Energy Cosmic Rays & Super-heavy Dark Matter

    CERN Document Server

    Marzola, Luca

    2016-01-01

    We reanalyse the prospects for upcoming Ultra-High Energy Cosmic Ray experiments in connection with the phenomenology of Super-heavy Dark Matter. We identify a set of observables well suited to reveal a possible anisotropy in the High Energy Cosmic Ray flux induced by the decays of these particles, and quantify their performance via Monte Carlo simulations that mimic the outcome of near-future and next-generation experiments. The spherical and circular dipoles are able to tell isotropic and anisotropic fluxes apart at a confidence level as large as $4\\sigma$ or $5\\sigma$, depending on the Dark Matter profile. The forward-to-backward flux ratio yields a comparable result for relatively large opening angles of about 40~deg, but it is less performing once a very large number of events is considered. We also find that an actual experiment employing these observables and collecting 300~events at 60~EeV would have a $50\\%$ chance of excluding isotropy against Super-heavy Dark Matter at a significance of at least $3...

  15. High temperature condensate clouds in super-hot Jupiter atmospheres

    CERN Document Server

    Wakeford, Hannah R; Lewis, Nikole K; Kataria, Tiffany; Marley, Mark S; Fortney, Jonathan J; Mandell, Avi M

    2016-01-01

    Deciphering the role of clouds is central to our understanding of exoplanet atmospheres, as they have a direct impact on the temperature and pressure structure, and observational properties of the planet. Super-hot Jupiters occupy a temperature regime similar to low mass M-dwarfs, where minimal cloud condensation is expected. However, observations of exoplanets such as WASP-12b (Teq ~ 2500 K) result in a transmission spectrum indicative of a cloudy atmosphere. We re-examine the temperature and pressure space occupied by these super-hot Jupiter atmospheres, to explore the role of the initial Al- and Ti-bearing condensates as the main source of cloud material. Due to the high temperatures a majority of the more common refractory material is not depleted into deeper layers and would remain in the vapor phase. The lack of depletion into deeper layers means that these materials with relatively low cloud masses can become significant absorbers in the upper atmosphere. We provide condensation curves for the initial ...

  16. Highly sensitive and multiplexed platforms for allergy diagnostics

    Science.gov (United States)

    Monroe, Margo R.

    Allergy is a disorder of the immune system caused by an immune response to otherwise harmless environmental allergens. Currently 20% of the US population is allergic and 90% of pediatric patients and 60% of adult patients with asthma have allergies. These percentages have increased by 18.5% in the past decade, with predicted similar trends for the future. Here we design sensitive, multiplexed platforms to detect allergen-specific IgE using the Interferometric Reflectance Imaging Sensor (IRIS) for various clinical settings. A microarray platform for allergy diagnosis allows for testing of specific IgE sensitivity to a multitude of allergens, while requiring only small volumes of patient blood sample. However, conventional fluorescent microarray technology is limited by i) the variation of probe immobilization, which hinders the ability to make quantitative, assertive, and statistically relevant conclusions necessary in immunodiagnostics and ii) the use of fluorophore labels, which is not suitable for some clinical applications due to the tendency of fluorophores to stick to blood particulates and require daily calibration methods. This calibrated fluorescence enhancement (CaFE) method integrates the low magnification modality of IRIS with enhanced fluorescence sensing in order to directly correlate immobilized probe (major allergens) density to allergen-specific IgE in patient serum. However, this platform only operates in processed serum samples, which is not ideal for point of care testing. Thus, a high magnification modality of IRIS was adapted as an alternative allergy diagnostic platform to automatically discriminate and size single nanoparticles bound to specific IgE in unprocessed, characterized human blood and serum samples. These features make IRIS an ideal candidate for clinical and diagnostic applications, such a POC testing. The high magnification (nanoparticle counting) modality in conjunction with low magnification of IRIS in a combined instrument

  17. Multiplexed communication over a high-speed quantum channel

    CERN Document Server

    Heurs, M; Dunlop, A E; Harb, C C; Ralph, T C; Huntington, E H

    2010-01-01

    In quantum information systems it is of particular interest to consider the best way in which to use the non-classical resources consumed by that system. Quantum communication protocols are integral to quantum information systems and are amongst the most promising near-term applications of quantum information science. Here we show that a multiplexed, digital quantum communications system supported by comb of vacuum squeezing has a greater channel capacity per photon than a source of broadband squeezing with the same analogue bandwidth. We report on the time-resolved, simultaneous observation of the first dozen teeth in a 2.4 GHz comb of vacuum squeezing produced by a sub-threshold OPO, as required for such a quantum communications channel. We also demonstrate multiplexed communication on that channel.

  18. Implementation of digital multiplexing for high resolution X-ray detector arrays.

    Science.gov (United States)

    Sharma, P; Swetadri Vasan, S N; Titus, A H; Cartwright, A N; Bednarek, D R; Rudin, S

    2012-01-01

    We describe and demonstrate for the first time the use of the novel Multiple Module Multiplexer (MMMIC) for a 2×2 array of new electron multiplying charge coupled device (EMCCD) based x-ray detectors. It is highly desirable for x-ray imaging systems to have larger fields of view (FOV) extensible in two directions yet to still be capable of doing high resolution imaging over regions-of-interest (ROI). The MMMIC achieves these goals by acquiring and multiplexing data from an array of imaging modules thereby enabling a larger FOV, and at the same time allowing high resolution ROI imaging through selection of a subset of modules in the array. MMMIC also supports different binning modes. This paper describes how a specific two stage configuration connecting three identical MMMICs is used to acquire and multiplex data from a 2×2 array of EMCCD based detectors. The first stage contains two MMMICs wherein each MMMIC is getting data from two EMCCD detectors. The multiplexed data from these MMMICs is then forwarded to the second stage MMMIC in the similar fashion. The second stage that has only one MMMIC gives the final 12 bit multiplexed data from four modules. This data is then sent over a high speed Camera Link interface to the image processing computer. X-ray images taken through the 2×2 array of EMCCD based detectors using this two stage configuration of MMMICs are shown successfully demonstrating the concept.

  19. The use of HEMTs in multiplexing large arrays of high impedance LTDs

    Energy Technology Data Exchange (ETDEWEB)

    Yates, S.J.C. [CNRS-CRTBT, 25 Rue des Martyrs, 38042 Grenoble Cedex 9 (France)]. E-mail: stephen.yates@grenoble.cnrs.fr; Benoit, A. [CNRS-CRTBT, 25 Rue des Martyrs, 38042 Grenoble Cedex 9 (France); Jin, Y. [CNRS-LPN, Route de Nozay, 91461 Marcoussis (France); Camus, Ph. [CNRS-CRTBT, 25 Rue des Martyrs, 38042 Grenoble Cedex 9 (France); Cavanna, A. [CNRS-LPN, Route de Nozay, 91461 Marcoussis (France); Durand, T. [CNRS-CRTBT, 25 Rue des Martyrs, 38042 Grenoble Cedex 9 (France); Etienne, B. [CNRS-LPN, Route de Nozay, 91461 Marcoussis (France); Gennser, U. [CNRS-LPN, Route de Nozay, 91461 Marcoussis (France); Gremion, E. [CNRS-LPN, Route de Nozay, 91461 Marcoussis (France); Hoffmann, C. [CNRS-CRTBT, 25 Rue des Martyrs, 38042 Grenoble Cedex 9 (France); Universite Joseph Fourier, Grenoble (France); Leclercq, S. [CNRS-CRTBT, 25 Rue des Martyrs, 38042 Grenoble Cedex 9 (France); Ulysse, Ch. [CNRS-LPN, Route de Nozay, 91461 Marcoussis (France)

    2006-04-15

    The use of a multiplexing system in close proximity to the detectors simplifies the electronics associated with large arrays of Low Temperature Detectors (LTDs). Here, we report the demonstration of an array of Quantum-Point-Contact High-Electron-Mobility-Transistors (QPC-HEMTs) down to 100mK used to time multiplex the signal from 8 simulated high impedance LTDs ({approx}10M{omega}) to an individual amplifier. Capacitors in parallel with the individual LTDs integrate the signal between measurements so giving a quasi-DC measurement of the LTDs. With the high impedance of the LTD this acts as a filter which counter acts the aliasing of the Johnson noise of the LTD associated with the time-multiplexing technique.

  20. An angle encoder for super-high resolution and super-high accuracy using SelfA

    Science.gov (United States)

    Watanabe, Tsukasa; Kon, Masahito; Nabeshima, Nobuo; Taniguchi, Kayoko

    2014-06-01

    Angular measurement technology at high resolution for applications such as in hard disk drive manufacturing machines, precision measurement equipment and aspherical process machines requires a rotary encoder with high accuracy, high resolution and high response speed. However, a rotary encoder has angular deviation factors during operation due to scale error or installation error. It has been assumed to be impossible to achieve accuracy below 0.1″ in angular measurement or control after the installation onto the rotating axis. Self-calibration (Lu and Trumper 2007 CIRP Ann. 56 499; Kim et al 2011 Proc. MacroScale; Probst 2008 Meas. Sci. Technol. 19 015101; Probst et al Meas. Sci. Technol. 9 1059; Tadashi and Makoto 1993 J. Robot. Mechatronics 5 448; Ralf et al 2006 Meas. Sci. Technol. 17 2811) and cross-calibration (Probst et al 1998 Meas. Sci. Technol. 9 1059; Just et al 2009 Precis. Eng. 33 530; Burnashev 2013 Quantum Electron. 43 130) technologies for a rotary encoder have been actively discussed on the basis of the principle of circular closure. This discussion prompted the development of rotary tables which achieve reliable and high accuracy angular verification. We apply these technologies for the development of a rotary encoder not only to meet the requirement of super-high accuracy but also to meet that of super-high resolution. This paper presents the development of an encoder with 221 = 2097 152 resolutions per rotation (360°), that is, corresponding to a 0.62″ signal period, achieved by the combination of a laser rotary encoder supplied by Magnescale Co., Ltd and a self-calibratable encoder (SelfA) supplied by The National Institute of Advanced Industrial Science & Technology (AIST). In addition, this paper introduces the development of a rotary encoder to guarantee ±0.03″ accuracy at any point of the interpolated signal, with respect to the encoder at the minimum resolution of 233, that is, corresponding to a 0.0015″ signal period after

  1. Application of HEMT for multiplexing large arrays of high impedance LTDs

    Energy Technology Data Exchange (ETDEWEB)

    Benoit, A.; Camus, Ph. E-mail: camus@grenoble.cnrs.fr; Cavanna, A.; Elhdiy, A.; Jin, Y.; Leclercq, S

    2004-03-11

    The development of large arrays of detectors requires using a proximate electronics at low temperatures for signal multiplexing and amplification. We report the use of commercial High Electronic Mobility Transistors (HEMT) for multiplexing signals from high impedance LTD arrays (typ. 10-100 M{omega}). The electronic architecture is based on HEMTs cooled at 0.1 K for the multiplexer and a JFET amplifier cooled at 100 K with <1 nV/Hz{sup 1/2} noise figure. For this time-multiplexing scheme, a capacitor is used to integrate the signal between measurements. Two main solutions are compared for the detectors polarization: the first uses a classical resistive method; the second uses a common capacitor allowing to polarize the detectors individually. The multiplexing ratio is mainly limited by the amplifier noise to about 20 detectors per amplifier with a pixel sampling rate of about 60 Hz. A specific development of HEMT arrays with Quantum Point Contacts (QPC-HEMT) with very small grid-to-channel capacitor ({approx}1 fF) allows minimizing the transient effects and realizing a close integration with the LTD arrays.

  2. SuperB: A High-Luminosity Asymmetric e+e- Super Flavor Factory

    Energy Technology Data Exchange (ETDEWEB)

    Bona, M.; /et al.

    2007-05-18

    We discuss herein the exciting physics program that can be accomplished with a very large sample of heavy quark and heavy lepton decays produced in the very clean environment of an e{sup +}e{sup -} collider; a program complementary to that of an experiment such as LHCb at a hadronic machine. It then presents the conceptual design of a new type of e{sup +}e{sup -} collider that produces a nearly two-order-of-magnitude increase in luminosity over the current generation of asymmetric B Factories. The key idea is the use of low emittance beams produced in an accelerator lattice derived from the ILC Damping Ring Design, together with a new collision region, again with roots in the ILC final focus design, but with important new concepts developed in this design effort. Remarkably, SuperB produces this very large improvement in luminosity with circulating currents and wallplug power similar to those of the current B Factories. There is clear synergy with ILC R&D; design efforts have already influenced one another, and many aspects of the ILC Damping Rings and Final Focus would be operationally tested at SuperB. Finally, the design of an appropriate detector, based on an upgrade of BABAR as an example, is discussed in some detail. A preliminary cost estimate is presented, as is an example construction timeline.

  3. Advances and Prospects in Breeding Japonica Rice for Super High Yield in the Northern China

    Institute of Scientific and Technical Information of China (English)

    CHEN Wen-fu; XU Zheng-jin; ZHANG Wen-zhong

    2007-01-01

    In this paper, advances and prospects in breeding japonica rice for super high yield in the northern China were analyzed comprehensively in terms of breeding theories, techniques and practices. The author holds that developing and spreading super rice is an important way to enhance the overall yielding ability of japonica rice and attaining immense expansion of rice production. After theories and technical guidelines for super rice breeding were formulated, which involved the creation of new plant morphology and strong hybrid vigor through crossing indica with japonica subspecies, the optimization of combination of desirable traits via multiple crossing or backcrossing, the assemblage of favorable genes and the integration of ideal plant morphology with the utilization of vigor-major breakthroughs have been made in conventional breeding of japonica super rice. A batch of new super rice varieties marked by superior rice quality and high disease resistance, such as Shennong 265, Shennong 606, and Jijing 88, etc., have been developed and released. In comparison with the advancement in conventional breeding of super rice, progress in hybrid japonica super rice breeding is slower because of climatic and ecological constraint in northern China. Therefore, solving the contradictions between vigor and growth duration, between yield and rice quality, and boosting vastly seed production are still serious challenges for breeders of hybrid japonica rice. Physiological and genetic problems in japonica super rice breeding are also discussed in this paper.

  4. Scalable multiplexed detector system for high-rate telecom-band single-photon detection.

    Science.gov (United States)

    Brida, G; Degiovanni, I P; Piacentini, F; Schettini, V; Polyakov, S V; Migdall, A

    2009-11-01

    We present an actively multiplexed photon-counting detection system at telecom wavelengths that overcomes the difficulties of photon-counting at high rates. We find that for gated detectors, the heretofore unconsidered deadtime associated with the detector gate is a critical parameter, that limits the overall scalability of the scheme to just a few detectors. We propose and implement a new scheme that overcomes this problem and restores full scalability that allows an order of magnitude improvement with systems with as few as 4 detectors. When using just two multiplexed detectors, our experimental results show a 5x improvement over a single detector and a greater than 2x improvement over multiplexed schemes that do not consider gate deadtime.

  5. Next Generation Molecular Histology Using Highly Multiplexed Ion Beam Imaging (MIBI) of Breast Cancer Tissue Specimens for Enhanced Clinical Guidance

    Science.gov (United States)

    2016-07-01

    AWARD NUMBER: W81XWH- 14-1-0192 TITLE: Next-Generation Molecular Histology Using Highly Multiplexed Ion Beam Imaging (MIBI) of Breast Cancer...DATES COVERED 4. TITLE AND SUBTITLE Next-Generation Molecular Histology Using Highly Multiplexed Ion Beam Imaging (MIBI) of Breast Cancer Tissue

  6. Fast Super-Resolution Imaging with Ultra-High Labeling Density Achieved by Joint Tagging Super-Resolution Optical Fluctuation Imaging (JT-SOFI)

    CERN Document Server

    Zeng, Zhiping; Wang, Hening; Huang, Ning; Shan, Chunyan; Zhang, Hao; Teng, Junlin; Xi, Peng

    2015-01-01

    Previous stochastic localization-based super-resolution techniques are largely limited by the labeling density and the fidelity to the morphology of specimen. We report on an optical super-resolution imaging scheme implementing joint tagging using multiple fluorescent blinking dyes associated with super-resolution optical fluctuation imaging (JT-SOFI), achieving ultra-high labeling density super-resolution imaging. To demonstrate the feasibility of JT-SOFI, quantum dots with different emission spectra were jointly labeled to the tubulin in COS7 cells, creating ultra-high density labeling. After analyzing and combining the fluorescence intermittency images emanating from spectrally resolved quantum dots, the microtubule networks are capable of being investigated with high fidelity and remarkably enhanced contrast at sub-diffraction resolution. The spectral separation also significantly decreased the frame number required for SOFI, enabling fast super-resolution microscopy through simultaneous data acquisition....

  7. Highly reconfigurable communication protocol multiplexing element for SCOPH

    Science.gov (United States)

    Brebner, Gordon

    2001-07-01

    The Soft Circuitry Optimised Protocol Harness (SCOPH) project is concerned with implementing streaming data paths that perform communication protocol functions. The data paths are implemented dynamically using soft circuitry on a programmed logic array, based upon a small set of parameterised function blocks that are instantiated and linked together on a per-connection basis, and may also be modified during connections. This allows the use of optimised bespoke protocols, with handling of protocol functions at hardware speeds. This paper focuses on one particular pair of function blocks, for the demultiplexing and multiplexing of multiple connections sharing a single communication channel. An example would be multiple TCP port connections simultaneously active over a single IP channel. In the SCOPH context, a single data stream emanating from a main processor or from a network interface would be split into multiple data streams, which undergo protocol processing, and are then recombined into a single stream to a network interface or main processor respectively. The demultiplexor block always requires a means of selecting one of a number of streams, given addressing information in the data stream itself. Symmetrically, the multiplexor block must insert appropriate addressing information into the data stream. However, the main thrust of the paper is in comparing area-time trade-offs between using blocks with a (small) fixed number of streams always configured and using blocks that vary in size and shape with the current number of active streams. Both options are being investigated on a Xilinx Virtex FPGA, using JBits for the dynamic configuration.

  8. Research Advances in High-Yielding Cultivation and Physiology of Super Rice

    Institute of Scientific and Technical Information of China (English)

    FU Jing; YANG Jian-chang

    2012-01-01

    In 1996,China launched a program to breed super rice or super hybrid rice by combining intersubspecific heterosis with ideal plant types.Today,approximately 80 super rice varieties have been released and some of them show high grain yields of 12-21 t/hm2 in field experiments.The main reasons for the high yields of super rice varieties,compared with those of conventional varieties,can be summarized as follows:more spikelets per panicle and larger sink size (number of spikelets per square meter); larger leaf area index,longer duration of green leaf,greater photosynthetic rate,higher lodging resistance,greater dry matter accumulation before the heading stage,greater remobilization of pre-stored carbohydrates from stems and leaves to grains during the grain-filling period; and larger root system and greater root activity.However,there are two main problems in super rice production:poor grain-filling of the later-flowering inferior spikelets (in contrast to earlier-flowering superior spikelets),and low and unstable seed-setting rate.Here,we review recent research advances in the crop physiology of super rice,focusing on biological features,formation of yield components,and population quality.Finally,we suggest further research on crop physiology of super rice.

  9. Combining target enrichment with barcode multiplexing for high throughput SNP discovery

    Directory of Open Access Journals (Sweden)

    Lunke Sebastian

    2010-11-01

    Full Text Available Abstract Background The primary goal of genetic linkage analysis is to identify genes affecting a phenotypic trait. After localisation of the linkage region, efficient genetic dissection of the disease linked loci requires that functional variants are identified across the loci. These functional variations are difficult to detect due to extent of genetic diversity and, to date, incomplete cataloguing of the large number of variants present both within and between populations. Massively parallel sequencing platforms offer unprecedented capacity for variant discovery, however the number of samples analysed are still limited by cost per sample. Some progress has been made in reducing the cost of resequencing using either multiplexing methodologies or through the utilisation of targeted enrichment technologies which provide the ability to resequence genomic areas of interest rather that full genome sequencing. Results We developed a method that combines current multiplexing methodologies with a solution-based target enrichment method to further reduce the cost of resequencing where region-specific sequencing is required. Our multiplex/enrichment strategy produced high quality data with nominal reduction of sequencing depth. We undertook a genotyping study and were successful in the discovery of novel SNP alleles in all samples at uniplex, duplex and pentaplex levels. Conclusion Our work describes the successful combination of a targeted enrichment method and index barcode multiplexing to reduce costs, time and labour associated with processing large sample sets. Furthermore, we have shown that the sequencing depth obtained is adequate for credible SNP genotyping analysis at uniplex, duplex and pentaplex levels.

  10. High-Speed Microscale Optical Tracking Using Digital Frequency-Domain Multiplexing

    OpenAIRE

    MacLachlan, Robert A.; Riviere, Cameron N.

    2009-01-01

    Position-sensitive detectors (PSDs), or lateral-effect photodiodes, are commonly used for high-speed, high-resolution optical position measurement. This paper describes the instrument design for multidimensional position and orientation measurement based on the simultaneous position measurement of multiple modulated sources using frequency-domain-multiplexed (FDM) PSDs. The important advantages of this optical configuration in comparison with laser/mirror combinations are that it has a large ...

  11. High-Speed Microscale Optical Tracking Using Digital Frequency-Domain Multiplexing

    OpenAIRE

    MacLachlan, Robert A.; Riviere, Cameron N.

    2009-01-01

    Position-sensitive detectors (PSDs), or lateral-effect photodiodes, are commonly used for high-speed, high-resolution optical position measurement. This paper describes the instrument design for multidimensional position and orientation measurement based on the simultaneous position measurement of multiple modulated sources using frequency-domain-multiplexed (FDM) PSDs. The important advantages of this optical configuration in comparison with laser/mirror combinations are that it has a large ...

  12. A novel lab-on-chip platform with integrated solid phase PCR and Supercritical Angle Fluorescence (SAF) microlens array for highly sensitive and multiplexed pathogen detection.

    Science.gov (United States)

    Hung, Tran Quang; Chin, Wai Hoe; Sun, Yi; Wolff, Anders; Bang, Dang Duong

    2017-04-15

    Solid-phase PCR (SP-PCR) has become increasingly popular for molecular diagnosis and there have been a few attempts to incorporate SP-PCR into lab-on-a-chip (LOC) devices. However, their applicability for on-line diagnosis is hindered by the lack of sensitive and portable on-chip optical detection technology. In this paper, we addressed this challenge by combining the SP-PCR with super critical angle fluorescence (SAF) microlens array embedded in a microchip. We fabricated miniaturized SAF microlens array as part of a microfluidic chamber in thermoplastic material and performed multiplexed SP-PCR directly on top of the SAF microlens array. Attribute to the high fluorescence collection efficiency of the SAF microlens array, the SP-PCR assay on the LOC platform demonstrated a high sensitivity of 1.6 copies/µL, comparable to off-chip detection using conventional laser scanner. The combination of SP-PCR and SAF microlens array allows for on-chip highly sensitive and multiplexed pathogen detection with low-cost and compact optical components. The LOC platform would be widely used as a high-throughput biosensor to analyze food, clinical and environmental samples.

  13. SUPER-THIN COATING MATERIAL FOR HIGH-GRADE HIGHWAY

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The road surface of cement concrete in highway is easily cracked and even destroyed due to inhomogeneous subsiding of the road foundation. In this work, a super-thin-coating material is prepared in order to repair the destroyed thin road surface, in which polymers and steel-fibers are added into ordinary concrete to form a steel fiber reinforced polymer-cement-based composite. the composite was successfully used to repair road surface. Microstructure and mechanical properties of the composites are measured and analyzed.

  14. Code Division Multiplexing Using AI Based Custom Constellation Scheme - Efficient Modulation for High Data rate Transmission

    Directory of Open Access Journals (Sweden)

    K.SESHADRI SASTRY,

    2010-06-01

    Full Text Available To achieve high bit rate in transmission over wireless channels frequency reuse is an encouraging concept . Rather than dividing allocated frequency spectrum into narrow band width channels , one for each user information is transmitted over a very wide frequency spectrum using the same carrier frequency within same frequency band . In this paper we propose code division multiplexing scheme in which Custom QAM modulator itself is used as code . The propose system is simulated and tested in Matlab 7.4.

  15. Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging.

    Science.gov (United States)

    Zeng, Zhiping; Chen, Xuanze; Wang, Hening; Huang, Ning; Shan, Chunyan; Zhang, Hao; Teng, Junlin; Xi, Peng

    2015-02-10

    Previous stochastic localization-based super-resolution techniques are largely limited by the labeling density and the fidelity to the morphology of specimen. We report on an optical super-resolution imaging scheme implementing joint tagging using multiple fluorescent blinking dyes associated with super-resolution optical fluctuation imaging (JT-SOFI), achieving ultra-high labeling density super-resolution imaging. To demonstrate the feasibility of JT-SOFI, quantum dots with different emission spectra were jointly labeled to the tubulin in COS7 cells, creating ultra-high density labeling. After analyzing and combining the fluorescence intermittency images emanating from spectrally resolved quantum dots, the microtubule networks are capable of being investigated with high fidelity and remarkably enhanced contrast at sub-diffraction resolution. The spectral separation also significantly decreased the frame number required for SOFI, enabling fast super-resolution microscopy through simultaneous data acquisition. As the joint-tagging scheme can decrease the labeling density in each spectral channel, thereby bring it closer to single-molecule state, we can faithfully reconstruct the continuous microtubule structure with high resolution through collection of only 100 frames per channel. The improved continuity of the microtubule structure is quantitatively validated with image skeletonization, thus demonstrating the advantage of JT-SOFI over other localization-based super-resolution methods.

  16. Fast Super-Resolution Imaging with Ultra-High Labeling Density Achieved by Joint Tagging Super-Resolution Optical Fluctuation Imaging

    Science.gov (United States)

    Zeng, Zhiping; Chen, Xuanze; Wang, Hening; Huang, Ning; Shan, Chunyan; Zhang, Hao; Teng, Junlin; Xi, Peng

    2015-01-01

    Previous stochastic localization-based super-resolution techniques are largely limited by the labeling density and the fidelity to the morphology of specimen. We report on an optical super-resolution imaging scheme implementing joint tagging using multiple fluorescent blinking dyes associated with super-resolution optical fluctuation imaging (JT-SOFI), achieving ultra-high labeling density super-resolution imaging. To demonstrate the feasibility of JT-SOFI, quantum dots with different emission spectra were jointly labeled to the tubulin in COS7 cells, creating ultra-high density labeling. After analyzing and combining the fluorescence intermittency images emanating from spectrally resolved quantum dots, the microtubule networks are capable of being investigated with high fidelity and remarkably enhanced contrast at sub-diffraction resolution. The spectral separation also significantly decreased the frame number required for SOFI, enabling fast super-resolution microscopy through simultaneous data acquisition. As the joint-tagging scheme can decrease the labeling density in each spectral channel, thereby bring it closer to single-molecule state, we can faithfully reconstruct the continuous microtubule structure with high resolution through collection of only 100 frames per channel. The improved continuity of the microtubule structure is quantitatively validated with image skeletonization, thus demonstrating the advantage of JT-SOFI over other localization-based super-resolution methods. PMID:25665878

  17. Fast and highly specific DNA-based multiplex detection on a solid support.

    Science.gov (United States)

    Barišić, Ivan; Kamleithner, Verena; Schönthaler, Silvia; Wiesinger-Mayr, Herbert

    2015-01-01

    Highly specific and fast multiplex detection methods are essential to conduct reasonable DNA-based diagnostics and are especially important to characterise infectious diseases. More than 1000 genetic targets such as antibiotic resistance genes, virulence factors and phylogenetic markers have to be identified as fast as possible to facilitate the correct treatment of a patient. In the present work, we developed a novel ligation-based DNA probe concept that was combined with the microarray technology and used it for the detection of bacterial pathogens. The novel linear chain (LNC) probes identified all tested species correctly within 1 h based on their 16S rRNA gene in a 25-multiplex reaction. Genomic DNA was used directly as template in the ligation reaction identifying as little as 10(7) cells without any pre-amplification. The high specificity was further demonstrated characterising a single nucleotide polymorphism leading to no false positive fluorescence signals of the untargeted single nucleotide polymorphism (SNP) variants. In comparison to conventional microarray probes, the sensitivity of the novel LNC3 probes was higher by a factor of 10 or more. In summary, we present a fast, simple, highly specific and sensitive multiplex detection method adaptable for a wide range of applications.

  18. Temporal and spatial multiplexed infrared single-photon counter based on high-speed avalanche photodiode

    Science.gov (United States)

    Chen, Xiuliang; Ding, Chengjie; Pan, Haifeng; Huang, Kun; Laurat, Julien; Wu, Guang; Wu, E.

    2017-03-01

    We report on a high-speed temporal and spatial multiplexed single-photon counter with photon-number-resolving capability up to four photons. The infrared detector combines a fiber loop to split, delay and recombine optical pulses and a 200 MHz dual-channel single-photon detector based on InGaAs/InP avalanche photodiode. To fully characterize the photon-number-resolving capability, we perform quantum detector tomography and then reconstruct its positive-operator-valued measure and the associated Wigner functions. The result shows that, despite of the afterpulsing noise and limited system detection efficiency, this temporal and spatial multiplexed single-photon counter can already find applications for large repetition rate quantum information schemes.

  19. Temporal and spatial multiplexed infrared single-photon counter based on high-speed avalanche photodiode

    Science.gov (United States)

    Chen, Xiuliang; Ding, Chengjie; Pan, Haifeng; Huang, Kun; Laurat, Julien; Wu, Guang; Wu, E

    2017-01-01

    We report on a high-speed temporal and spatial multiplexed single-photon counter with photon-number-resolving capability up to four photons. The infrared detector combines a fiber loop to split, delay and recombine optical pulses and a 200 MHz dual-channel single-photon detector based on InGaAs/InP avalanche photodiode. To fully characterize the photon-number-resolving capability, we perform quantum detector tomography and then reconstruct its positive-operator-valued measure and the associated Wigner functions. The result shows that, despite of the afterpulsing noise and limited system detection efficiency, this temporal and spatial multiplexed single-photon counter can already find applications for large repetition rate quantum information schemes. PMID:28294155

  20. The effect of water stress on super-high- density 'Koroneiki' olive oil quality.

    Science.gov (United States)

    Dag, Arnon; Naor, Amos; Ben-Gal, Alon; Harlev, Guy; Zipori, Isaac; Schneider, Doron; Birger, Reuven; Peres, Moti; Gal, Yoni; Kerem, Zohar

    2015-08-15

    Over the last two decades, the area of cultivated super-high-density olive orchards has increased rapidly. Water stress is an important tool in super-high-density orchards to reduce tree growth and promote suitability for overhead mechanical harvesters. Little is known regarding the effect of water stress in super-high-density orchards on oil quality parameters. In this study the effect of irrigation rate on oil quality parameters was evaluated in a six-year-old super-high-density 'Koreneiki' olive orchard for five consecutive seasons. Five water status levels, determined by irrigating in order to maintain various midday stem water potential threshold values (-1.5, -2, -2.5, -3 and -4 MPa), were applied during the oil accumulation stage. The MUFA/PUFA ratio and free fatty acid content generally decreased as a function of increasing tree water stress. In most seasons a reduction in polyphenols was found with decreasing irrigation level. Peroxide value was not affected by the water stress level. The present study demonstrates that limiting irrigation and exposure of olive trees to water stress in a super-high-density orchard lowers free fatty acid content and therefore benefits oil quality. However, the decreased MUFA/PUFA ratio and the reduction in polyphenol content that were also found under increased water stress negatively influence oil quality. © 2014 Society of Chemical Industry.

  1. Generating barcoded libraries for multiplex high-throughput sequencing.

    Science.gov (United States)

    Knapp, Michael; Stiller, Mathias; Meyer, Matthias

    2012-01-01

    Molecular barcoding is an essential tool to use the high throughput of next generation sequencing platforms optimally in studies involving more than one sample. Various barcoding strategies allow for the incorporation of short recognition sequences (barcodes) into sequencing libraries, either by ligation or polymerase chain reaction (PCR). Here, we present two approaches optimized for generating barcoded sequencing libraries from low copy number extracts and amplification products typical of ancient DNA studies.

  2. Recent advances in high-capacity free-space optical and radio-frequency communications using orbital angular momentum multiplexing

    Science.gov (United States)

    Willner, Alan E.; Ren, Yongxiong; Xie, Guodong; Yan, Yan; Li, Long; Zhao, Zhe; Wang, Jian; Tur, Moshe; Molisch, Andreas F.; Ashrafi, Solyman

    2017-02-01

    There is a continuing growth in the demand for data bandwidth, and the multiplexing of multiple independent data streams has the potential to provide the needed data capacity. One technique uses the spatial domain of an electromagnetic (EM) wave, and space division multiplexing (SDM) has become increasingly important for increased transmission capacity and spectral efficiency of a communication system. A subset of SDM is mode division multiplexing (MDM), in which multiple orthogonal beams each on a different mode can be multiplexed. A potential modal basis set to achieve MDM is to use orbital angular momentum (OAM) of EM waves. In such a system, multiple OAM beams each carrying an independent data stream are multiplexed at the transmitter, propagate through a common medium and are demultiplexed at the receiver. As a result, the total capacity and spectral efficiency of the communication system can be multiplied by a factor equal to the number of transmitted OAM modes. Over the past few years, progress has been made in understanding the advantages and limitations of using multiplexed OAM beams for communication systems. In this review paper, we highlight recent advances in the use of OAM multiplexing for high-capacity free-space optical and millimetre-wave communications. We discuss different technical challenges (e.g. atmospheric turbulence and crosstalk) as well as potential techniques to mitigate such degrading effects. This article is part of the themed issue 'Optical orbital angular momentum'.

  3. Recent advances in high-capacity free-space optical and radio-frequency communications using orbital angular momentum multiplexing.

    Science.gov (United States)

    Willner, Alan E; Ren, Yongxiong; Xie, Guodong; Yan, Yan; Li, Long; Zhao, Zhe; Wang, Jian; Tur, Moshe; Molisch, Andreas F; Ashrafi, Solyman

    2017-02-28

    There is a continuing growth in the demand for data bandwidth, and the multiplexing of multiple independent data streams has the potential to provide the needed data capacity. One technique uses the spatial domain of an electromagnetic (EM) wave, and space division multiplexing (SDM) has become increasingly important for increased transmission capacity and spectral efficiency of a communication system. A subset of SDM is mode division multiplexing (MDM), in which multiple orthogonal beams each on a different mode can be multiplexed. A potential modal basis set to achieve MDM is to use orbital angular momentum (OAM) of EM waves. In such a system, multiple OAM beams each carrying an independent data stream are multiplexed at the transmitter, propagate through a common medium and are demultiplexed at the receiver. As a result, the total capacity and spectral efficiency of the communication system can be multiplied by a factor equal to the number of transmitted OAM modes. Over the past few years, progress has been made in understanding the advantages and limitations of using multiplexed OAM beams for communication systems. In this review paper, we highlight recent advances in the use of OAM multiplexing for high-capacity free-space optical and millimetre-wave communications. We discuss different technical challenges (e.g. atmospheric turbulence and crosstalk) as well as potential techniques to mitigate such degrading effects.This article is part of the themed issue 'Optical orbital angular momentum'. © 2017 The Author(s).

  4. Super-resonant radiation stimulated by high-harmonics

    CERN Document Server

    Loures, Cristian Redondo; Faccio, Daniele; Biancalana, Fabio

    2016-01-01

    Solitons propagating in media with higher order dispersion will shed radiation known as dispersive wave or resonant radiation, with applications in frequency broadening, deep UV sources for spectroscopy or simply fundamental studies of soliton physics. Starting from a recently proposed equation that models the behaviour of ultrashort optical pulses in nonlinear materials using the analytic signal, we find that the resonant radiation associated with the third-harmonic generation term of the equation is parametrically stimulated with an unprecedented gain. Resonant radiation levels, typically only a small fraction of the soliton, are now as intense as the soliton itself. The mechanism is quite universal and works also in normal dispersion and with harmonics higher than the third. We report experimental hints of this super-resonant radiation stimulated by the fifth harmonic in diamond.

  5. Development of a multiplexed readout with high position resolution for positron emission tomography

    Science.gov (United States)

    Lee, Sangwon; Choi, Yong; Kang, Jihoon; Jung, Jin Ho

    2017-04-01

    Detector signals for positron emission tomography (PET) are commonly multiplexed to reduce the number of digital processing channels so that the system can remain cost effective while also maintaining imaging performance. In this work, a multiplexed readout combining Anger position estimation algorithm and position decoder circuit (PDC) was developed to reduce the number of readout channels by a factor of 24, 96-to-4. The data acquisition module consisted of a TDC (50 ps resolution), 4-channel ADCs (12 bit, 105 MHz sampling rate), 2 GB SDRAM and USB3.0. The performance of the multiplexed readout was assessed with a high-resolution PET detector block composed of 2×3 detector modules, each consisting of an 8×8 array of 1.52×1.52×6 mm3 LYSO, a 4×4 array of 3×3 mm2 silicon photomultiplier (SiPM) and 13.4×13.4 mm2 light guide with 0.7 mm thickness. The acquired flood histogram showed that all 384 crystals could be resolved. The average energy resolution at 511 keV was 13.7±1.6% full-width-at-half-maximum (FWHM) and the peak-to-valley ratios of the flood histogram on the horizontal and vertical lines were 18.8±0.8 and 22.8±1.3, respectively. The coincidence resolving time of a pair of detector blocks was 6.2 ns FWHM. The reconstructed phantom image showed that rods down to a diameter of 1.6 mm could be resolved. The results of this study indicate that the multiplexed readout would be useful in developing a PET with a spatial resolution less than the pixel size of the photosensor, such as a SiPM array.

  6. High Resolution Bathymetry Estimation Improvement with Single ImageSuper Resolution Algorithm Super Resolution Forests

    Science.gov (United States)

    2017-01-26

    process of the SRF algorithm, we were able to further increase the mean PSNR score of the high resolution estimated data from previously used bicubic...This meant that implementing the edited variance before the bicubic estimates were created caused the mean PSNR to increase the most, and all...interpolation (by about 1 dB). Figure 7: PSNR comparison (with mean scores) between Bicubic Interpolation and SRF Figure 7 shows the comparison between

  7. Development of a Fluorescent Multiplex Assay for Detection of MSI-High Tumors

    Directory of Open Access Journals (Sweden)

    Jeffery W. Bacher

    2004-01-01

    of MSI testing in that it is both extremely sensitive and specific and amenable to high-throughput analysis. The MSI Multiplex System meets the new recommendations proposed at the recent 2002 NCI workshop on HNPCC and MSI testing and overcomes problems inherent to the original five-marker panel. The use of a single multiplex fluorescent MSI assay reduces the time and costs involved in MSI testing with increased reliability and accuracy and thus should facilitate widespread screening for microsatellite instability in tumors of patients with gastrointestinal cancers.

  8. Astronomy. ASASSN-15lh: A highly super-luminous supernova.

    Science.gov (United States)

    Dong, Subo; Shappee, B J; Prieto, J L; Jha, S W; Stanek, K Z; Holoien, T W-S; Kochanek, C S; Thompson, T A; Morrell, N; Thompson, I B; Basu, U; Beacom, J F; Bersier, D; Brimacombe, J; Brown, J S; Bufano, F; Chen, Ping; Conseil, E; Danilet, A B; Falco, E; Grupe, D; Kiyota, S; Masi, G; Nicholls, B; Olivares E, F; Pignata, G; Pojmanski, G; Simonian, G V; Szczygiel, D M; Woźniak, P R

    2016-01-15

    We report the discovery of ASASSN-15lh (SN 2015L), which we interpret as the most luminous supernova yet found. At redshift z = 0.2326, ASASSN-15lh reached an absolute magnitude of Mu ,AB = -23.5 ± 0.1 and bolometric luminosity Lbol = (2.2 ± 0.2) × 10(45) ergs s(-1), which is more than twice as luminous as any previously known supernova. It has several major features characteristic of the hydrogen-poor super-luminous supernovae (SLSNe-I), whose energy sources and progenitors are currently poorly understood. In contrast to most previously known SLSNe-I that reside in star-forming dwarf galaxies, ASASSN-15lh appears to be hosted by a luminous galaxy (MK ≈ -25.5) with little star formation. In the 4 months since first detection, ASASSN-15lh radiated (1.1 ± 0.2) × 10(52) ergs, challenging the magnetar model for its engine. Copyright © 2016, American Association for the Advancement of Science.

  9. Highly super-Chandrasekhar white dwarfs in an extensive GRMHD framework

    CERN Document Server

    Das, Upasana

    2016-01-01

    Our consistent effort to unravel the mystery of super-Chandrasekhar white dwarfs (WDs), by exploiting the potential of magnetic fields, has brought this topic considerable attention. This is also evident from the recent surge in the corresponding literature. In the present work, by means of full-scale general relativistic magnetohydrodynamic (GRMHD) numerical analysis, we confirm the existence of stable, highly magnetized, significantly super-Chandrasekhar WDs having mass exceeding 3 solar mass. We have explored various possible field configurations, namely, poloidal, toroidal and mixed, by self-consistently incorporating the departure from spherical symmetry induced by a strong magnetic field. Such super-Chandrasekhar WDs can be ideal progenitors of peculiar, over-luminous type Ia supernovae.

  10. Multiplex APLP System for High-Resolution Haplogrouping of Extremely Degraded East-Asian Mitochondrial DNAs

    Science.gov (United States)

    Kakuda, Tsuneo; Shojo, Hideki; Tanaka, Mayumi; Nambiar, Phrabhakaran; Minaguchi, Kiyoshi; Umetsu, Kazuo; Adachi, Noboru

    2016-01-01

    Mitochondrial DNA (mtDNA) serves as a powerful tool for exploring matrilineal phylogeographic ancestry, as well as for analyzing highly degraded samples, because of its polymorphic nature and high copy numbers per cell. The recent advent of complete mitochondrial genome sequencing has led to improved techniques for phylogenetic analyses based on mtDNA, and many multiplex genotyping methods have been developed for the hierarchical analysis of phylogenetically important mutations. However, few high-resolution multiplex genotyping systems for analyzing East-Asian mtDNA can be applied to extremely degraded samples. Here, we present a multiplex system for analyzing mitochondrial single nucleotide polymorphisms (mtSNPs), which relies on a novel amplified product-length polymorphisms (APLP) method that uses inosine-flapped primers and is specifically designed for the detailed haplogrouping of extremely degraded East-Asian mtDNAs. We used fourteen 6-plex polymerase chain reactions (PCRs) and subsequent electrophoresis to examine 81 haplogroup-defining SNPs and 3 insertion/deletion sites, and we were able to securely assign the studied mtDNAs to relevant haplogroups. Our system requires only 1×10−13 g (100 fg) of crude DNA to obtain a full profile. Owing to its small amplicon size (<110 bp), this new APLP system was successfully applied to extremely degraded samples for which direct sequencing of hypervariable segments using mini-primer sets was unsuccessful, and proved to be more robust than conventional APLP analysis. Thus, our new APLP system is effective for retrieving reliable data from extremely degraded East-Asian mtDNAs. PMID:27355212

  11. Simulation and Experimental Analysis of Super High-Speed Grinding of Ductile Material

    Institute of Scientific and Technical Information of China (English)

    SHIMIZU; Jun; EDA; Hiroshi

    2002-01-01

    This study aims to reduce the work-affected layer of the machined surface by carrying out the grinding at the speed over static pr o pagation speed of plastic wave of ductile materials and also aims to clarify suc h super high-speed machining mechanism.This paper reports on the result obtain ed through the molecular dynamics simulations and experiments on the super-spee d grinding below and beyond static propagation speed of aluminum.From the simul ation results,it is verified that the plastic deformation ...

  12. High Resolution Pulse Compression Imaging Using Super Resolution FM-Chirp Correlation Method (SCM)

    Science.gov (United States)

    Fujiwara, M.; Okubo, K.; Tagawa, N.

    This study addresses the issue of the super-resolution pulse compression technique (PCT) for ultrasound imaging. Time resolution of multiple ultrasonic echoes using the FM-Chirp PCT is limited by the bandwidth of the sweep-frequency. That is, the resolution depends on the sharpness of auto-correlation function. We propose the Super resolution FM-Chirp correlation Method (SCM) and evaluate its performance. This method is based on the multiple signal classification (MUSIC) algorithm. Our simulations were made for the model assuming multiple signals reflected from some scatterers. We confirmed that SCM detects time delay of complicated reflected signals successfully with high resolution.

  13. Multiplexed homogeneous proximity ligation assays for high throughput protein biomarker research in serological material

    DEFF Research Database (Denmark)

    Lundberg, Martin; Thorsen, Stine Buch; Assarsson, Erika;

    2011-01-01

    A high throughput protein biomarker discovery tool has been developed based on multiplexed proximity ligation assays (PLA) in a homogeneous format in the sense of no washing steps. The platform consists of four 24-plex panels profiling 74 putative biomarkers with sub pM sensitivity each consuming...... only 1 micro Litre of human plasma sample. The system uses either matched monoclonal antibody pairs or the more readily available single batches of affinity purified polyclonal antibodies to generate the target specific reagents by covalently linking with unique nucleic acid sequences. These paired...

  14. Movable thin films with embedded high-index microspheres for super-resolution microscopy

    CERN Document Server

    Allen, Kenneth W; Li, Yangcheng; Limberopoulos, Nicholaos I; Walker, Dennis E; Urbas, Augustine M; Liberman, Vladimir; Astratov, Vasily N

    2015-01-01

    Microsphere-assisted imaging emerged as a surprisingly simple way of achieving optical super-resolution imaging. In this work, we use movable PDMS thin films with embedded high-index barium titanate glass microspheres a sample scanning capability was developed, thus removing the main limitation of this technology based on its small field-of-view.

  15. High resolution FISH on super-stretched flow-sorted plant chromosomes.

    NARCIS (Netherlands)

    Valárik, M.; Bartos, J.; Kovarova, P.; Kubalakova, M.; Jong, de J.H.S.G.M.; Dolezel, J.

    2004-01-01

    A novel high-resolution fluorescence in situ hybridisation (FISH) strategy, using super-stretched flow-sorted plant chromosomes as targets, is described. The technique that allows longitudinal extension of chromosomes of more than 100 times their original metaphase size is especially attractive for

  16. Applications of Luminex xMAP technology for rapid, high-throughput multiplexed nucleic acid detection.

    Science.gov (United States)

    Dunbar, Sherry A

    2006-01-01

    As we enter the post-genome sequencing era and begin to sift through the enormous amount of genetic information now available, the need for technologies that allow rapid, cost-effective, high-throughput detection of specific nucleic acid sequences becomes apparent. Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can greatly reduce the time, cost and labor associated with single reaction detection technologies. The Luminex xMAP system is a multiplexed microsphere-based suspension array platform capable of analyzing and reporting up to 100 different reactions in a single reaction vessel. This technology provides a new platform for high-throughput nucleic acid detection and is being utilized with increasing frequency. Here we review specific applications of xMAP technology for nucleic acid detection in the areas of single nucleotide polymorphism (SNP) genotyping, genetic disease screening, gene expression profiling, HLA DNA typing and microbial detection. These studies demonstrate the speed, efficiency and utility of xMAP technology for simultaneous, rapid, sensitive and specific nucleic acid detection, and its capability to meet the current and future requirements of the molecular laboratory for high-throughput nucleic acid detection.

  17. High-resolution and super stacking of time-reversal mirrors in locating seismic sources

    KAUST Repository

    Cao, Weiping

    2011-07-08

    Time reversal mirrors can be used to backpropagate and refocus incident wavefields to their actual source location, with the subsequent benefits of imaging with high-resolution and super-stacking properties. These benefits of time reversal mirrors have been previously verified with computer simulations and laboratory experiments but not with exploration-scale seismic data. We now demonstrate the high-resolution and the super-stacking properties in locating seismic sources with field seismic data that include multiple scattering. Tests on both synthetic data and field data show that a time reversal mirror has the potential to exceed the Rayleigh resolution limit by factors of 4 or more. Results also show that a time reversal mirror has a significant resilience to strong Gaussian noise and that accurate imaging of source locations from passive seismic data can be accomplished with traces having signal-to-noise ratios as low as 0.001. Synthetic tests also demonstrate that time reversal mirrors can sometimes enhance the signal by a factor proportional to the square root of the product of the number of traces, denoted as N and the number of events in the traces. This enhancement property is denoted as super-stacking and greatly exceeds the classical signal-to-noise enhancement factor of. High-resolution and super-stacking are properties also enjoyed by seismic interferometry and reverse-time migration with the exact velocity model. © 2011 European Association of Geoscientists & Engineers.

  18. Multiplex high-throughput gene mutation analysis in acute myeloid leukemia.

    Science.gov (United States)

    Dunlap, Jennifer; Beadling, Carol; Warrick, Andrea; Neff, Tanaya; Fleming, William H; Loriaux, Marc; Heinrich, Michael C; Kovacsovics, Tibor; Kelemen, Katalin; Leeborg, Nicky; Gatter, Ken; Braziel, Rita M; Press, Richard; Corless, Christopher L; Fan, Guang

    2012-12-01

    Classification of acute myeloid leukemia increasingly depends on genetic analysis. However, the number of known mutations in acute myeloid leukemia is expanding rapidly. Therefore, we tested a high-throughput screening method for acute myeloid leukemia mutation analysis using a multiplex mass spectrometry-based approach. To our knowledge, this is the first reported application of this approach to genotype leukemias in a clinical setting. One hundred seven acute myeloid leukemia cases were screened for mutations using a panel that covers 344 point mutations across 31 genes known to be associated with leukemia. The analysis was performed by multiplex polymerase chain reaction for mutations in genes of interest followed by primer extension reactions. Products were analyzed on a Sequenom MassARRAY system (San Diego, CA). The multiplex panel yielded mutations in 58% of acute myeloid leukemia cases with normal cytogenetics and 21% of cases with abnormal cytogenetics. Cytogenetics and routine polymerase chain reaction-based screening of NPM1, CEBPA, FLT3-ITD, and KIT was also performed on a subset of cases. When combined with the results of these standard polymerase chain reaction-based tests, the mutation frequency reached 78% in cases with normal cytogenetics. Of these, 42% harbored multiple mutations primarily involving NPM1 with NRAS, KRAS, CEBPA, PTPN11, IDH1, or FLT3. In contrast, cases with abnormal cytogenetics rarely harbored more than 1 mutation (1.5%), suggesting different underlying biology. This study demonstrates the feasibility and utility of broad-based mutation profiling of acute myeloid leukemia in a clinical setting. This approach will be helpful in defining prognostic subgroups of acute myeloid leukemia and contribute to the selection of patients for enrollment into trials with novel inhibitors.

  19. Highly multiplexible thermal kinetic inductance detectors for x-ray imaging spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Ulbricht, Gerhard, E-mail: ulbricht@physics.ucsb.edu; Mazin, Benjamin A.; Szypryt, Paul; Walter, Alex B.; Bockstiegel, Clint [Department of Physics, University of California, Santa Barbara, California 93106 (United States); Bumble, Bruce [NASA Jet Propulsion Laboratory, 4800 Oak Grove Drive, Pasadena, California 91125 (United States)

    2015-06-22

    For X-ray imaging spectroscopy, high spatial resolution over a large field of view is often as important as high energy resolution, but current X-ray detectors do not provide both in the same device. Thermal Kinetic Inductance Detectors (TKIDs) are being developed as they offer a feasible way to combine the energy resolution of transition edge sensors with pixel counts approaching CCDs and thus promise significant improvements for many X-ray spectroscopy applications. TKIDs are a variation of Microwave Kinetic Inductance Detectors (MKIDs) and share their multiplexibility: working MKID arrays with 2024 pixels have recently been demonstrated and much bigger arrays are under development. In this work, we present a TKID prototype, which is able to achieve an energy resolution of 75 eV at 5.9 keV, even though its general design still has to be optimized. We further describe TKID fabrication, characterization, multiplexing, and working principle and demonstrate the necessity of a data fitting algorithm in order to extract photon energies. With further design optimizations, we expect to be able to improve our TKID energy resolution to less than 10 eV at 5.9 keV.

  20. Highly Multiplexible Thermal Kinetic Inductance Detectors for X-Ray Imaging Spectroscopy

    CERN Document Server

    Ulbricht, Gerhard; Szypryt, Paul; Walter, Alex B; Bockstiegel, Clint; Bumble, Bruce

    2015-01-01

    For X-ray imaging spectroscopy, high spatial resolution over a large field of view is often as important as high energy resolution, but current X-ray detectors do not provide both in the same device. Thermal Kinetic Inductance Detectors (TKIDs) are being developed as they offer a feasible way to combine the energy resolution of transition edge sensors with pixel counts approaching CCDs and thus promise significant improvements for many X-ray spectroscopy applications. TKIDs are a variation of Microwave Kinetic Inductance Detectors (MKIDs) and share their multiplexibility: working MKID arrays with 2024 pixels have recently been demonstrated and much bigger arrays are under development. In this work, we present our first working TKID prototypes which are able to achieve an energy resolution of 75 eV at 5.9 keV, even though their general design still has to be optimized. We further describe TKID fabrication, characterization, multiplexing and working principle and demonstrate the necessity of a data fitting algo...

  1. "Paterniplex", a highly discriminative decaplex STR multiplex tailored for investigating special problems in paternity testing.

    Science.gov (United States)

    Betz, Thomas; Immel, Uta-Dorothee; Kleiber, Manfred; Klintschar, Michael

    2007-11-01

    The goal of the study was to develop a STR multiplex ("Paterniplex") that is--as supplement to commercially available multiplex kits like the Identifiler kit (Applied Biosystems, Foster City, CA)--suitable for solving complex paternity cases such as deficiency cases or cases with mutations. The Paterniplex comprises the nine highly polymorphic STRs D8S1132, D7S1517, D10S2325, D12S391, Se33, D17S976, Penta E, Penta D and FGA in addition to Amelogenin as sex determination marker. The loci were selected because of their high degree of polymorphism (higher than that of the widely used TH01 marker). Only one locus, FGA, is shared with the Identifiler kit to avoid sample mix up. The study further gives details on the population genetics of the loci in a German Caucasian population (allelic distribution, Hardy-Weinberg Equilibrium and forensic efficiency markers such as the Discriminating Power) and three examples for cases that could not be solved using commercially available kits alone, but using the Paterniplex in addition to a commercial kit.

  2. A Study of the Key Techniques of High-Speed Optical Time Division Multiplexing Systems

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@Several key techniques in high-speed optical time division multiplexing (OTDM) systems, including the generation and compression of gain-switched picosecond optical pulses, the all optical demultiplexer based on a nonlinear optical loop mirror (NOLM), terahertz optical asymmetric demultiplexer (TOAD) and the electroabsorption modulator in OTDM systems, are studied in this dissertation. The gain-switching characteristics and the nonlinear dynamics of a semiconductor laser are investigated by solving its single-mode rate equations numerically, highlighting the dependence of the pulse width and the peak power of a gain-switched pulse on the parameters such as the differential gain and the photon lifetime, and the effects of the DC bias current and the modulation current of the laser on the pulse width and the spectral width of the gain-switched pulse. The two-dimensional bifurcation diagram is computed in a large modulation current and modulation frequency range, and it is found that the low-frequency period doubling (multipling) and chaos region are not closed fully and outspread to the high modulation current direction with a narrow modulation frequency region. In addition, a new clock recovery scheme employing the period multipling is proposed. Besides compressing the gain-switched laser diode pulse can be compressed to 6.9ps by using this DILM compressor, and such a short pulse completely satisfies the requirement for a 40 Gbit/s optical time division multiplexing (OTDM) system.

  3. MassCode liquid arrays as a tool for multiplexed high-throughput genetic profiling.

    Directory of Open Access Journals (Sweden)

    Gregory S Richmond

    Full Text Available Multiplexed detection assays that analyze a modest number of nucleic acid targets over large sample sets are emerging as the preferred testing approach in such applications as routine pathogen typing, outbreak monitoring, and diagnostics. However, very few DNA testing platforms have proven to offer a solution for mid-plexed analysis that is high-throughput, sensitive, and with a low cost per test. In this work, an enhanced genotyping method based on MassCode technology was devised and integrated as part of a high-throughput mid-plexing analytical system that facilitates robust qualitative differential detection of DNA targets. Samples are first analyzed using MassCode PCR (MC-PCR performed with an array of primer sets encoded with unique mass tags. Lambda exonuclease and an array of MassCode probes are then contacted with MC-PCR products for further interrogation and target sequences are specifically identified. Primer and probe hybridizations occur in homogeneous solution, a clear advantage over micro- or nanoparticle suspension arrays. The two cognate tags coupled to resultant MassCode hybrids are detected in an automated process using a benchtop single quadrupole mass spectrometer. The prospective value of using MassCode probe arrays for multiplexed bioanalysis was demonstrated after developing a 14plex proof of concept assay designed to subtype a select panel of Salmonella enterica serogroups and serovars. This MassCode system is very flexible and test panels can be customized to include more, less, or different markers.

  4. High capacity fiber optic sensor networks using hybrid multiplexing techniques and their applications

    Science.gov (United States)

    Sun, Qizhen; Li, Xiaolei; Zhang, Manliang; Liu, Qi; Liu, Hai; Liu, Deming

    2013-12-01

    Fiber optic sensor network is the development trend of fiber senor technologies and industries. In this paper, I will discuss recent research progress on high capacity fiber sensor networks with hybrid multiplexing techniques and their applications in the fields of security monitoring, environment monitoring, Smart eHome, etc. Firstly, I will present the architecture of hybrid multiplexing sensor passive optical network (HSPON), and the key technologies for integrated access and intelligent management of massive fiber sensor units. Two typical hybrid WDM/TDM fiber sensor networks for perimeter intrusion monitor and cultural relics security are introduced. Secondly, we propose the concept of "Microstructure-Optical X Domin Refecltor (M-OXDR)" for fiber sensor network expansion. By fabricating smart micro-structures with the ability of multidimensional encoded and low insertion loss along the fiber, the fiber sensor network of simple structure and huge capacity more than one thousand could be achieved. Assisted by the WDM/TDM and WDM/FDM decoding methods respectively, we built the verification systems for long-haul and real-time temperature sensing. Finally, I will show the high capacity and flexible fiber sensor network with IPv6 protocol based hybrid fiber/wireless access. By developing the fiber optic sensor with embedded IPv6 protocol conversion module and IPv6 router, huge amounts of fiber optic sensor nodes can be uniquely addressed. Meanwhile, various sensing information could be integrated and accessed to the Next Generation Internet.

  5. Development of a New Foxtail Millet Germplasm with Super Early Maturity and High Iron Content

    Institute of Scientific and Technical Information of China (English)

    LIU Zheng-li; SUN Shi-xian; CHENG Ru-hong; HUANG Wen-sheng; LIU Jun-xin; QU Zhu-feng; XIA Xue-yan; SHI Zhi-gang

    2006-01-01

    Super Early Maturation No.2 is a new foxtail millet germplasm developed by using the targeted-character-gene-bankbreeding method. It has several outstanding characteristics. (1) Super early maturity. It can normally mature in Bashang,Hebei Province, China, where the altitude is around 1 400 meters, and it needs 1 650℃ of effective accumulated temperature in the growing period. (2) Millet is rich in iron. The iron content of the millet of Super Early Maturation No.2 is 54.10 mg kg-1,which is 62.0% higher than the average iron content of the foxtail millet varieties in China. (3) High crude fat content. Its crude fat content is 6.24%, which is 54.1% higher than the average content of foxtail millet varieties in China. (4) High crude protein content. The average of the crude protein content of all the foxtail millet varieties in China is 12.71%, only5% varieties surpass 14%, however, the crude protein content of Super Early Maturation No.2 is 14.36%, which is rare in improved varieties of foxtail millet in China. (5) Overall characteristics are good.

  6. High sensitive photonic crystal multiplexed biosensor array using H0 sandwiched cavities

    Science.gov (United States)

    Arafa, Safia; Bouchemat, Mohamed; Bouchemat, Touraya; Benmerkhi, Ahlem

    2017-03-01

    We theoretically investigate a high sensitive photonic crystal integrated biosensor array structure which is potentially used for label-free multiplexed sensing. The proposed device consists of an array of three sandwiched H0 cavities patterned above silicon on insulator (SOI) substrate; each cavity has been designed for different cavity spacing and different resonant wavelength. Results obtained by performing finite-difference time-domain (FDTD) simulations, indicate that the response of each detection unit shifts independently in terms of refractive index variations. The optimized design makes possible the combination of sensing as a function of location, as well as a function of time in the same platform. A refractive index sensitivity of 520nm/RIU and a quality factor over 104 are both achieved with an accompanied crosstalk of less than -26 dB. In addition, the device presents an improved detection limit (DL) of 1.24.10-6 RIU and a wide measurement range. These features make the designed device a promising element for performing label-free multiplexed detection in monolithic substrate for medical diagnostics and environmental monitoring.

  7. High sensitive photonic crystal multiplexed biosensor array using H0 sandwiched cavities

    Directory of Open Access Journals (Sweden)

    Arafa Safia

    2017-01-01

    Full Text Available We theoretically investigate a high sensitive photonic crystal integrated biosensor array structure which is potentially used for label-free multiplexed sensing. The proposed device consists of an array of three sandwiched H0 cavities patterned above silicon on insulator (SOI substrate; each cavity has been designed for different cavity spacing and different resonant wavelength. Results obtained by performing finite-difference time-domain (FDTD simulations, indicate that the response of each detection unit shifts independently in terms of refractive index variations. The optimized design makes possible the combination of sensing as a function of location, as well as a function of time in the same platform. A refractive index sensitivity of 520nm/RIU and a quality factor over 104 are both achieved with an accompanied crosstalk of less than -26 dB. In addition, the device presents an improved detection limit (DL of 1.24.10-6 RIU and a wide measurement range. These features make the designed device a promising element for performing label-free multiplexed detection in monolithic substrate for medical diagnostics and environmental monitoring.

  8. Hologram multiplexing in a highly photosensitive photopolymerizable material in a sol-gel matrix

    Science.gov (United States)

    Murciano, A.; Blaya, S.; Carretero, L.; Ulibarrena, M.; Fimia, A.

    2005-07-01

    In this paper, we present a photopolymerizable material in a sol-gel matrix suitable for recording a large number of multiplexed diffraction gratings with low total exposure energy. The matrix of this material is an inorganic porous silica glass, a material that does not shrink when radical photopolymerization is initiated. The photosensitive component is based on acrylamide, N,N‧-methylenebisacrylamide, triethanolamine and yellow eosine as a photoinitiator. In the reported study, 50 holograms were angularly multiplexed with diffraction efficiencies between 0.1 and 1.9% resulting in a dynamic range of M/# = 3.9. The total exposure was 4.5 mJ/cm2 and the energy used in recording each hologram was 95 μJ/cm2. This indicates a very high sensitivity for this material in the range of 3.3 to 15.5 cm/mJ. Due to this good holographic performance, the material is suitable for holographic data storage applications.

  9. Low complexity digital backpropagation for high baud subcarrier-multiplexing systems.

    Science.gov (United States)

    Zhang, Fangyuan; Zhuge, Qunbi; Qiu, Meng; Plant, David V

    2016-07-25

    In this paper, we propose two modifications to reduce the complexity of the subcarrier-multiplexing (SCM) based digital backpropagation (DBP) for high symbol rate SCM systems. The first one is to reduce the number of interfering subcarriers (RS-SCM-DBP) when evaluating the cross-subcarrier nonlinearity (CSN). The second one is to replace the original frequency domain CSN filters with the infinite impulse response (IIR) filters (IIR-RS-SCM-DBP) in the CSN compensation. The performance of the proposed schemes are numerically evaluated in three-channel dual-polarization (DP) 16QAM wavelength-division multiplexing (WDM) transmissions. The aggregate symbol rate for each channel is 120 GBaud and the transmission distance is 1600 km. For the SCM system with 16 subcarriers, the IIR-RS-SCM-DBP with only 4 interfering subcarriers and 2 steps can achieve a 0.3 dB Q-factor improvement in the WDM transmission. Compared to the original SCM-DBP, the proposed IIR-RS-SCM-DBP reduces the complexity by 48% at a performance loss of only 0.07 dB.

  10. Growth and development characteristics of super-high-yielding mid-season japonica rice

    Institute of Scientific and Technical Information of China (English)

    YANG Jianchang; DU Yong; WU Changfu; LIU Lijun; WANG Zhiqin; ZHU Qingsen

    2007-01-01

    Rice is one of the most important food crops in China.The realization of the super-high-yielding (SHY)type has great significance in ensuring food security in this country.This study investigated the growth and development characteristics of the super-high-yielding rice (grain yield>11 t/hm2).Four mid-season japonica rice cultivars (including lines):Lianjiajing 2,Huajing 5,0026 and 9823,were grown in the paddy field.Growth analysis was performed during the growth period,and yield components were determined at maturity.Results showed that SHY rice had more sipkelets per panicle and higher filled-grain percentage than the high-yielding rice (CK,grain yield 8.98-9.16 t/hm2).There was no significant difference in the 1 000-grain weight between the super-high-yielding and the CK.Super-high-yield rice exhib ited fewer tillers at the early growth stage (from transplanting to jointing),with a higher ratio of productive tillers to total tillers,when compared with the CK.The leaf area index (LAI),photosynthetic potential and dry matter accumulation were lower for the SHY rice than those for the CK at the early growth stage,and the differences were not significant between the two rice types at heading,but were greater in the former than the latter after heading.The root-shoot ratio at each growth stage,root bleedings from heading to maturity,grain-leaf ratio,translocation percentage of the matter from stems and sheaths and harvest index of super-high-yielding rice were greater than those of CK.The indexes for the growth and development of SHY mid-season rice population were suggested,i.e.total spikelets>4.5×104/m2,filled-grain percentage>90%,1 000-grain weight>26 g;ratio of productive tillers>80%,leaf area index at heading 7.5-8.0,photosynthetic potential during the whole growth period >22 t/hm2,harvest index>0.51;grain-leaf ratio (number of spikelets per cm2 leaf area)>0.58;root-shoot ratio at heading tion approaches and key cultivation techniques for raising the

  11. Fluorinated polymer with high thermal stability for fabrication of 32-channel arrayed waveguide grating multiplexer on silicon

    Institute of Scientific and Technical Information of China (English)

    WANG Fei; LI Ai-ze; SUN Wei; YI Mao-bin; JIANG Zhen-hua; LIU Shi-yong; ZHANG Da-ming

    2005-01-01

    A cross-linkable fluorinated poly (ether ether ketone) (FPEEK) was synthesized for the fabrication of arrayed waveguide grating (AWG) multiplexer. The results of thermal gravimetric analysis (TGA) and near-infrared absorption spectrum show that the materials have high thermal stability and high optical transparency in the infrared communication region. The refractive index of FPEEK can be controlled easily by changing the fluorine content of the materials. The 32-channel AWG multiplexer is fabricated using the FPEEK and oxygen reactive ion etching technology. The AWG multiplexer exhibits that the insertion loss is from 12.8 to 17.8 dB and the channel crosstalk is less than -20 dB. The wavelength channel spacing and the center wavelength are 0.8 nm and 1 548 nm, respectively.

  12. High throughput, multiplexed pathogen detection authenticates plague waves in medieval Venice, Italy.

    Science.gov (United States)

    Tran, Thi-Nguyen-Ny; Signoli, Michel; Fozzati, Luigi; Aboudharam, Gérard; Raoult, Didier; Drancourt, Michel

    2011-03-10

    Historical records suggest that multiple burial sites from the 14th-16th centuries in Venice, Italy, were used during the Black Death and subsequent plague epidemics. High throughput, multiplexed real-time PCR detected DNA of seven highly transmissible pathogens in 173 dental pulp specimens collected from 46 graves. Bartonella quintana DNA was identified in five (2.9%) samples, including three from the 16th century and two from the 15th century, and Yersinia pestis DNA was detected in three (1.7%) samples, including two from the 14th century and one from the 16th century. Partial glpD gene sequencing indicated that the detected Y. pestis was the Orientalis biotype. These data document for the first time successive plague epidemics in the medieval European city where quarantine was first instituted in the 14th century.

  13. High throughput, multiplexed pathogen detection authenticates plague waves in medieval Venice, Italy.

    Directory of Open Access Journals (Sweden)

    Thi-Nguyen-Ny Tran

    Full Text Available BACKGROUND: Historical records suggest that multiple burial sites from the 14th-16th centuries in Venice, Italy, were used during the Black Death and subsequent plague epidemics. METHODOLOGY/PRINCIPAL FINDINGS: High throughput, multiplexed real-time PCR detected DNA of seven highly transmissible pathogens in 173 dental pulp specimens collected from 46 graves. Bartonella quintana DNA was identified in five (2.9% samples, including three from the 16th century and two from the 15th century, and Yersinia pestis DNA was detected in three (1.7% samples, including two from the 14th century and one from the 16th century. Partial glpD gene sequencing indicated that the detected Y. pestis was the Orientalis biotype. CONCLUSIONS: These data document for the first time successive plague epidemics in the medieval European city where quarantine was first instituted in the 14th century.

  14. Effects of pattern dependence on high-power polarization-division-multiplexing applications

    Institute of Scientific and Technical Information of China (English)

    Anlin Yi; Lianshan Yan; Bin Luo; Wei Pan; Jia Ye

    2012-01-01

    High-power polarization-division-multiplexing (PDM) systems or functional modules,such as self-phasemodulation (SPM)-based all-optical regenerators,cross-phase-modulation (XPM)-based wavelength convertors or format convertors,all-optical logical gate,and so on,may suffer from the effects of pattern dependence.Such effects are experimentally investigated using relative time delay variation between bit sequences with orthogonal polarization states in a 2 × 10.65 Gb/s high-power on-off keying (OOK) PDM system.Eye-diagram-based signal-to-noise ratio (SNR) and bandwidth of broadened spectrum are measured and compared.An eye-diagram-based SNR fluctuation of up to 4 dB may occur as the delay changes.

  15. Discussion on Super High-temperature Weather and City Construction

    Institute of Scientific and Technical Information of China (English)

    OuYang Shoucheng; Wei Ming

    2006-01-01

    With the method of structure analysis, this work analyzes the real data of the high-temperature weather of China in 2003 and those in history, and finds out that the structure character of the hightemperature weather process corresponds to the distribution of urban buildings. The result shows that excessive dense buildings could influence the atmosphere structure, which leads to the urban temperature increasing sharply. On the other hand, the structure analysis also reveals some problems on urban construction, and the corresponding countermeasure is an efficient method for high-temperature weather forecast.

  16. Super soft silicone elastomers with high dielectric permittivity

    DEFF Research Database (Denmark)

    Madsen, Frederikke Bahrt; Yu, Liyun; Hvilsted, Søren;

    2015-01-01

    Dielectric elastomers (DEs) have many favourable properties. The obstacle of high driving voltages, however, limits the commercial viability of the technology at present. Driving voltage can be lowered by decreasing the Young’s modulus and increasing the dielectric permittivity of silicone elasto...

  17. High Spin Isomers and Super Heavy Elements (SHE) Synthesis

    Science.gov (United States)

    Popescu, Domitian G.

    2010-04-01

    To get closer to the SHE-Island the new radioactive beams are proposed for future fusion reaction. We suggest something different : to use the advantage of High Spin Isomer States, by tacking into account the importance of the G (spin-isospin cupling) suggested by Ripka 1.

  18. Super-Resolution Reconstruction of High-Resolution Satellite ZY-3 TLC Images.

    Science.gov (United States)

    Li, Lin; Wang, Wei; Luo, Heng; Ying, Shen

    2017-05-07

    Super-resolution (SR) image reconstruction is a technique used to recover a high-resolution image using the cumulative information provided by several low-resolution images. With the help of SR techniques, satellite remotely sensed images can be combined to achieve a higher-resolution image, which is especially useful for a two- or three-line camera satellite, e.g., the ZY-3 high-resolution Three Line Camera (TLC) satellite. In this paper, we introduce the application of the SR reconstruction method, including motion estimation and the robust super-resolution technique, to ZY-3 TLC images. The results show that SR reconstruction can significantly improve both the resolution and image quality of ZY-3 TLC images.

  19. Super-High Resolution Time Interval Measurement Method Based on Time-Space Relationships

    Institute of Scientific and Technical Information of China (English)

    DU Bao-Qiang; ZHOU Wei

    2009-01-01

    Based on the principle of quantized delay-time, a super-high resolution time interval measurement method is proposed based on time-space relationships.Using the delay-time stability that time and frequency signal travel in a specific medium, the measured time interval can be quantized.Combined with the phase coincidence detection technique, the measurement of time can be changed into the measurement of space length.The resolution and the stability of the measurement system are easily improved.Experimental results show that the measurement resolution of the measured time interval depends on the length difference of the double delay-time unit.When the length difference is set up on millimeter level or sub-millimeter level, super-high measurement resolution from hundreds of picosecond to tens of picosecond can be obtained.

  20. High contrast imaging of exoplanets on ELTs using a super-Nyquist wavefront control scheme

    CERN Document Server

    Gerard, Benjamin L

    2016-01-01

    One of the key science goals for extremely large telescopes (ELTs) is the detailed characterization of already known directly imaged exoplanets. The typical adaptive optics (AO) Nyquist control region for ELTs is ~0.4 arcseconds, placing many already known directly imaged planets outside the DM control region and not allowing any standard wavefront control scheme to remove speckles that would allow higher SNR images/spectra to be acquired. This can be fixed with super-Nyquist wavefront control (SNWFC), using a sine wave phase plate to allow for wavefront control outside the central DM Nyquist region. We demonstrate that SNWFC is feasible through a simple, deterministic, non-coronagraphic, super-Nyquist speckle nulling technique in the adaptive optics laboratory at the National Research Council of Canada. We also present results in simulation of how SNWFC using the self coherent camera (SCC) can be used for high contrast imaging. This technique could be implemented on future high contrast imaging instruments t...

  1. Multiplex enrichment quantitative PCR (ME-qPCR): a high-throughput, highly sensitive detection method for GMO identification.

    Science.gov (United States)

    Fu, Wei; Zhu, Pengyu; Wei, Shuang; Zhixin, Du; Wang, Chenguang; Wu, Xiyang; Li, Feiwu; Zhu, Shuifang

    2017-04-01

    Among all of the high-throughput detection methods, PCR-based methodologies are regarded as the most cost-efficient and feasible methodologies compared with the next-generation sequencing or ChIP-based methods. However, the PCR-based methods can only achieve multiplex detection up to 15-plex due to limitations imposed by the multiplex primer interactions. The detection throughput cannot meet the demands of high-throughput detection, such as SNP or gene expression analysis. Therefore, in our study, we have developed a new high-throughput PCR-based detection method, multiplex enrichment quantitative PCR (ME-qPCR), which is a combination of qPCR and nested PCR. The GMO content detection results in our study showed that ME-qPCR could achieve high-throughput detection up to 26-plex. Compared to the original qPCR, the Ct values of ME-qPCR were lower for the same group, which showed that ME-qPCR sensitivity is higher than the original qPCR. The absolute limit of detection for ME-qPCR could achieve levels as low as a single copy of the plant genome. Moreover, the specificity results showed that no cross-amplification occurred for irrelevant GMO events. After evaluation of all of the parameters, a practical evaluation was performed with different foods. The more stable amplification results, compared to qPCR, showed that ME-qPCR was suitable for GMO detection in foods. In conclusion, ME-qPCR achieved sensitive, high-throughput GMO detection in complex substrates, such as crops or food samples. In the future, ME-qPCR-based GMO content identification may positively impact SNP analysis or multiplex gene expression of food or agricultural samples. Graphical abstract For the first-step amplification, four primers (A, B, C, and D) have been added into the reaction volume. In this manner, four kinds of amplicons have been generated. All of these four amplicons could be regarded as the target of second-step PCR. For the second-step amplification, three parallels have been taken for

  2. SOWFA Super-Controller: A High-Fidelity Tool for Evaluating Wind Plant Control Approaches

    Energy Technology Data Exchange (ETDEWEB)

    Fleming, P.; Gebraad, P.; van Wingerden, J. W.; Lee, S.; Churchfield, M.; Scholbrock, A.; Michalakes, J.; Johnson, K.; Moriarty, P.

    2013-01-01

    This paper presents a new tool for testing wind plant controllers in the Simulator for Offshore Wind Farm Applications (SOWFA). SOWFA is a high-fidelity simulator for the interaction between wind turbine dynamics and the fluid flow in a wind plant. The new super-controller testing environment in SOWFA allows for the implementation of the majority of the wind plant control strategies proposed in the literature.

  3. Second Approximation Model for Optical Head in Super High Density Storage Technology

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The paper presents second approximation model for optical head in super high-density storage technology firstly and it is an important part for three grades approximate model of ultra-small-size quantum well corn-shaped laser and simulative calculations. It supplies the important and useful results for the NFOD optical head design with ultra thin active layer and ultra small spot laser.

  4. All-fibre multiplexed source of high-purity heralded single photons

    CERN Document Server

    Francis-Jones, Robert J A; Mosley, Peter J

    2016-01-01

    Single photon sources based on spontaneous photon-pair generation have enabled pioneering experiments in quantum optics. However, their non-determinism presents a bottleneck to scaling up photonic and hybrid quantum-enhanced technologies. Furthermore, photon pairs are typically emitted into many correlated frequency modes, producing an undesirable mixed state on heralding. Here we present a complete fibre-integrated heralded single photon source that addresses both these difficulties simultaneously. We use active switching to provide a path to deterministic operation by multiplexing separate spontaneous sources, and dispersion engineering to minimise frequency correlation for high-purity single photon generation. All the essential elements -- nonlinear material with dispersion control, wavelength isolation, optical delay, and fast switching -- are incorporated in a low-loss alignment-free package that heralds photons in telecoms single-mode fibre. Our results demonstrate a scalable approach to delivering pure...

  5. High-Speed Turbo-TCM-Coded Orthogonal Frequency-Division Multiplexing Ultra-Wideband Systems

    Directory of Open Access Journals (Sweden)

    2006-01-01

    Full Text Available One of the UWB proposals in the IEEE P802.15 WPAN project is to use a multiband orthogonal frequency-division multiplexing (OFDM system and punctured convolutional codes for UWB channels supporting a data rate up to 480 Mbps. In this paper, we improve the proposed system using turbo TCM with QAM constellation for higher data rate transmission. We construct a punctured parity-concatenated trellis codes, in which a TCM code is used as the inner code and a simple parity-check code is employed as the outer code. The result shows that the system can offer a much higher spectral efficiency, for example, 1.2 Gbps, which is 2.5 times higher than the proposed system. We identify several essential requirements to achieve the high rate transmission, for example, frequency and time diversity and multilevel error protection. Results are confirmed by density evolution.

  6. Advancing the High Throughput Identification of Liver Fibrosis Protein Signatures Using Multiplexed Ion Mobility Spectrometry*

    Science.gov (United States)

    Baker, Erin Shammel; Burnum-Johnson, Kristin E.; Jacobs, Jon M.; Diamond, Deborah L.; Brown, Roslyn N.; Ibrahim, Yehia M.; Orton, Daniel J.; Piehowski, Paul D.; Purdy, David E.; Moore, Ronald J.; Danielson, William F.; Monroe, Matthew E.; Crowell, Kevin L.; Slysz, Gordon W.; Gritsenko, Marina A.; Sandoval, John D.; LaMarche, Brian L.; Matzke, Melissa M.; Webb-Robertson, Bobbie-Jo M.; Simons, Brenna C.; McMahon, Brian J.; Bhattacharya, Renuka; Perkins, James D.; Carithers, Robert L.; Strom, Susan; Self, Steven G.; Katze, Michael G.; Anderson, Gordon A.; Smith, Richard D.

    2014-01-01

    Rapid diagnosis of disease states using less invasive, safer, and more clinically acceptable approaches than presently employed is a crucial direction for the field of medicine. While MS-based proteomics approaches have attempted to meet these objectives, challenges such as the enormous dynamic range of protein concentrations in clinically relevant biofluid samples coupled with the need to address human biodiversity have slowed their employment. Herein, we report on the use of a new instrumental platform that addresses these challenges by coupling technical advances in rapid gas phase multiplexed ion mobility spectrometry separations with liquid chromatography and MS to dramatically increase measurement sensitivity and throughput, further enabling future high throughput MS-based clinical applications. An initial application of the liquid chromatography - ion mobility spectrometry-MS platform analyzing blood serum samples from 60 postliver transplant patients with recurrent fibrosis progression and 60 nontransplant patients illustrates its potential utility for disease characterization. PMID:24403597

  7. Advancing the high throughput identification of liver fibrosis protein signatures using multiplexed ion mobility spectrometry.

    Science.gov (United States)

    Baker, Erin Shammel; Burnum-Johnson, Kristin E; Jacobs, Jon M; Diamond, Deborah L; Brown, Roslyn N; Ibrahim, Yehia M; Orton, Daniel J; Piehowski, Paul D; Purdy, David E; Moore, Ronald J; Danielson, William F; Monroe, Matthew E; Crowell, Kevin L; Slysz, Gordon W; Gritsenko, Marina A; Sandoval, John D; Lamarche, Brian L; Matzke, Melissa M; Webb-Robertson, Bobbie-Jo M; Simons, Brenna C; McMahon, Brian J; Bhattacharya, Renuka; Perkins, James D; Carithers, Robert L; Strom, Susan; Self, Steven G; Katze, Michael G; Anderson, Gordon A; Smith, Richard D

    2014-04-01

    Rapid diagnosis of disease states using less invasive, safer, and more clinically acceptable approaches than presently employed is a crucial direction for the field of medicine. While MS-based proteomics approaches have attempted to meet these objectives, challenges such as the enormous dynamic range of protein concentrations in clinically relevant biofluid samples coupled with the need to address human biodiversity have slowed their employment. Herein, we report on the use of a new instrumental platform that addresses these challenges by coupling technical advances in rapid gas phase multiplexed ion mobility spectrometry separations with liquid chromatography and MS to dramatically increase measurement sensitivity and throughput, further enabling future high throughput MS-based clinical applications. An initial application of the liquid chromatography--ion mobility spectrometry-MS platform analyzing blood serum samples from 60 postliver transplant patients with recurrent fibrosis progression and 60 nontransplant patients illustrates its potential utility for disease characterization.

  8. High-Speed Turbo-TCM-Coded Orthogonal Frequency-Division Multiplexing Ultra-Wideband Systems

    Directory of Open Access Journals (Sweden)

    Wang Yanxia

    2006-01-01

    Full Text Available One of the UWB proposals in the IEEE P802.15 WPAN project is to use a multiband orthogonal frequency-division multiplexing (OFDM system and punctured convolutional codes for UWB channels supporting a data rate up to 480 Mbps. In this paper, we improve the proposed system using turbo TCM with QAM constellation for higher data rate transmission. We construct a punctured parity-concatenated trellis codes, in which a TCM code is used as the inner code and a simple parity-check code is employed as the outer code. The result shows that the system can offer a much higher spectral efficiency, for example, 1.2 Gbps, which is 2.5 times higher than the proposed system. We identify several essential requirements to achieve the high rate transmission, for example, frequency and time diversity and multilevel error protection. Results are confirmed by density evolution.

  9. Highly multiplexed signal readout for a time-of-flight positron emission tomography detector based on silicon photomultipliers.

    Science.gov (United States)

    Cates, Joshua W; Bieniosek, Matthew F; Levin, Craig S

    2017-01-01

    Maintaining excellent timing resolution in the generation of silicon photomultiplier (SiPM)-based time-of-flight positron emission tomography (TOF-PET) systems requires a large number of high-speed, high-bandwidth electronic channels and components. To minimize the cost and complexity of a system's back-end architecture and data acquisition, many analog signals are often multiplexed to fewer channels using techniques that encode timing, energy, and position information. With progress in the development SiPMs having lower dark noise, after pulsing, and cross talk along with higher photodetection efficiency, a coincidence timing resolution (CTR) well below 200 ps FWHM is now easily achievable in single pixel, bench-top setups using 20-mm length, lutetium-based inorganic scintillators. However, multiplexing the output of many SiPMs to a single channel will significantly degrade CTR without appropriate signal processing. We test the performance of a PET detector readout concept that multiplexes 16 SiPMs to two channels. One channel provides timing information with fast comparators, and the second channel encodes both position and energy information in a time-over-threshold-based pulse sequence. This multiplexing readout concept was constructed with discrete components to process signals from a [Formula: see text] array of SensL MicroFC-30035 SiPMs coupled to [Formula: see text] Lu1.8Gd0.2SiO5 (LGSO):Ce (0.025 mol. %) scintillators. This readout method yielded a calibrated, global energy resolution of 15.3% FWHM at 511 keV with a CTR of [Formula: see text] FWHM between the 16-pixel multiplexed detector array and a [Formula: see text] LGSO-SiPM reference detector. In summary, results indicate this multiplexing scheme is a scalable readout technique that provides excellent coincidence timing performance.

  10. Development of a Parching Machine Using Super-Heated Vapor or Super-Heated High-Moisture Atmosphere

    Science.gov (United States)

    Sato, Shoichi; Shinsho, Seiji; Iriki, Hiroyuki; Asai, Junya; Suganuma, Hirofumi; Shibata, Tsutomu

    We developed a new parching machine with super-heated vapor or super-heated highmoisture atmosphere as a heat medium, and investigated the influence exerted on the characteristics of manufactured tea and crude tea quality. (1)We developed machine specifications that improved throughput and allowed us to control stable quality compared with the conventional kamairicha parching machine. (2)The new parching machine could not only manufacture like kamairicha but also achieve various degrees of steaming of products like green tea or heavily steamed sencha. (3)The new parching machine could not only deactivate enzymes but dry leaves. (4)The influence of throughput was great with respect to the grade of pan-parched flavour, which meant that there was a contact opportunity for tea leaves and the surface of machine's wall. (5)Unpleasant smells such as that produced in a summer crop of tea were reduced by the new parching machine.

  11. High core count single-mode multicore fiber for dense space division multiplexing

    DEFF Research Database (Denmark)

    Aikawa, K.; Sasaki, Y.; Amma, Y.;

    2016-01-01

    Multicore fibers and few-mode fibers have the potential to realize dense-space-division multiplexing systems. Several dense-space-division multiplexing system transmission experiments over multicore fibers and few-mode fibers have been demonstrated so far. Multicore fibers, including recent resul...

  12. Engineered nanoconstructs for the multiplexed and sensitive detection of high-risk pathogens

    Science.gov (United States)

    Seo, Youngmin; Kim, Ji-Eun; Jeong, Yoon; Lee, Kwan Hong; Hwang, Jangsun; Hong, Jongwook; Park, Hansoo; Choi, Jonghoon

    2016-01-01

    Many countries categorize the causative agents of severe infectious diseases as high-risk pathogens. Given their extreme infectivity and potential to be used as biological weapons, a rapid and sensitive method for detection of high-risk pathogens (e.g., Bacillus anthracis, Francisella tularensis, Yersinia pestis, and Vaccinia virus) is highly desirable. Here, we report the construction of a novel detection platform comprising two units: (1) magnetic beads separately conjugated with multiple capturing antibodies against four different high-risk pathogens for simple and rapid isolation, and (2) genetically engineered apoferritin nanoparticles conjugated with multiple quantum dots and detection antibodies against four different high-risk pathogens for signal amplification. For each high-risk pathogen, we demonstrated at least 10-fold increase in sensitivity compared to traditional lateral flow devices that utilize enzyme-based detection methods. Multiplexed detection of high-risk pathogens in a sample was also successful by using the nanoconstructs harboring the dye molecules with fluorescence at different wavelengths. We ultimately envision the use of this novel nanoprobe detection platform in future applications that require highly sensitive on-site detection of high-risk pathogens.

  13. Simultaneous measurements of auto-immune and infectious disease specific antibodies using a high throughput multiplexing tool.

    Directory of Open Access Journals (Sweden)

    Atul Asati

    Full Text Available Considering importance of ganglioside antibodies as biomarkers in various immune-mediated neuropathies and neurological disorders, we developed a high throughput multiplexing tool for the assessment of gangliosides-specific antibodies based on Biolpex/Luminex platform. In this report, we demonstrate that the ganglioside high throughput multiplexing tool is robust, highly specific and demonstrating ∼100-fold higher concentration sensitivity for IgG detection than ELISA. In addition to the ganglioside-coated array, the high throughput multiplexing tool contains beads coated with influenza hemagglutinins derived from H1N1 A/Brisbane/59/07 and H1N1 A/California/07/09 strains. Influenza beads provided an added advantage of simultaneous detection of ganglioside- and influenza-specific antibodies, a capacity important for the assay of both infectious antigen-specific and autoimmune antibodies following vaccination or disease. Taken together, these results support the potential adoption of the ganglioside high throughput multiplexing tool for measuring ganglioside antibodies in various neuropathic and neurological disorders.

  14. Variable-intercept panel model for deformation zoning of a super-high arch dam.

    Science.gov (United States)

    Shi, Zhongwen; Gu, Chongshi; Qin, Dong

    2016-01-01

    This study determines dam deformation similarity indexes based on an analysis of deformation zoning features and panel data clustering theory, with comprehensive consideration to the actual deformation law of super-high arch dams and the spatial-temporal features of dam deformation. Measurement methods of these indexes are studied. Based on the established deformation similarity criteria, the principle used to determine the number of dam deformation zones is constructed through entropy weight method. This study proposes the deformation zoning method for super-high arch dams and the implementation steps, analyzes the effect of special influencing factors of different dam zones on the deformation, introduces dummy variables that represent the special effect of dam deformation, and establishes a variable-intercept panel model for deformation zoning of super-high arch dams. Based on different patterns of the special effect in the variable-intercept panel model, two panel analysis models were established to monitor fixed and random effects of dam deformation. Hausman test method of model selection and model effectiveness assessment method are discussed. Finally, the effectiveness of established models is verified through a case study.

  15. High extinction ratio multiplexer/demultiplexer with a Mach-Zehnder interferometer and a fiber loop mirror

    Institute of Scientific and Technical Information of China (English)

    Yonglin Huang(黄勇林); Jie Li(李杰); Guiyun Kai(开桂云); Xiaoyi Dong(董孝义)

    2003-01-01

    A novel structure of high extinction ratio multiplexer/demultiplexer with a Mach-Zehnder interferometer (MZI) and a fiber loop mirror (FLM) is proposed. The experimental results show that the extinction ratio can be enhanced about two times in comparison with the conventional MZI.

  16. Photometric Redshifts for High Resolution Radio Galaxies in the SuperCLASS Field

    Science.gov (United States)

    Manning, Sinclaire; Casey, Caitlin; Battye, Richard; Hales, Christopher A.; Chapman, Scott; Smail, Ian; SuperCLASS Team

    2017-01-01

    SuperCLASS (the Super-Cluster Assisted Shear Survey) is a deep, wide-area (~2 square degrees) extragalactic field with high resolution (0.1”) radio continuum coverage from e-MERLIN (Multi-Element Radio Linked Interferometer Network.) The combination of sensitivity and spatial resolution make e-MERLIN an ideal tool to trace spatially resolved star-formation in heavily obscured, dusty star-forming galaxies (DSFGs). Plus, thanks to the tight relationship between radio continuum and far-IR observations we have an observationally inexpensive and accurate method of mapping star formation density in distant galaxies. We present a photometric redshift catalog for DSFGs located in the SuperCLASS field. Multiwavelength photometric data was obtained with Subaru SuprimeCam (B,V,r,i,z) and photometric redshifts were generated using the public photometric redshift code, EAZY. With these redshifts we aim to conduct the first large sample morphological analysis of z~1-3 obscured galaxies. We plan to address two important questions: 1) Are the majority of obscured SFR>50 Msolar/yr galaxies driven by major collisions? and 2) do luminous active galactic nuclei (AGN) play a crucial role in the quenching of highly obscured star-formation? These photometric redshifts are crucial in determining the physical origins of our DSFG sample and to also conduct radio weak lensing experiments with the e-MERLIN dataset.

  17. High sensitive immunoassay for multiplex mycotoxin detection with photonic crystal microsphere suspension array.

    Science.gov (United States)

    Deng, Guozhe; Xu, Kun; Sun, Yue; Chen, Yu; Zheng, Tiesong; Li, Jianlin

    2013-03-05

    A novel, sensitive, and high throughput competitive immunoassay for multiplex mycotoxins was established by immobilizing the artificial antigens (Ags) of mycotoxins on the surfaces of three kinds of silica photonic crystal microsphere (SPCM) suspension arrays. The SPCMs were encoded by their reflectance peak positions. Aflatoxin B1 (AFB1), fumonisin B1 (FB1), and citrinin (CIT) spiked in the cereals were extracted, and the fluorescein isothiocyanate (FITC) labeled antibodies (Abs) of these mycotoxins were added into the centrifuge tube which contained the SPCMs of the modified artificial antigens (Ags). The fluorescence signal was collected by an array fluorescent scanner. The limit of detection (LOD) was as low as 0.5, 1, and 0.8 pg/mL for AFB1, FB1, and CIT, respectively. The new method provided a wide linear detection range from 0.001 to 10, 0.001 to 10, and 0.001 to 1 ng/mL for AFB1, FB1, and CIT, respectively. The mean recovery rates are in range of 74.7 ± 4.0% to 127.9 ± 4.4% for the three mycotoxins in corn, peanuts, and wheat. The developed method for mycotoxins was used to assay the AFB1, FB1, and CIT level in 10 naturally contaminated cereal samples, and the results of detection were in agreement with that of a classic enzyme-linked immunosorbent assay (ELISA) method. This method saves a large amount of reagents (10 μL volume) and detection time (<3 h) for multiplex mycotoxin assay.

  18. Remote and high precision step height measurement with an optical fiber multiplexing interferometric system

    Science.gov (United States)

    Wang, Yunzhi; Xie, Fang; Ma, Sen; Chen, Liang

    2015-03-01

    An optical fiber multiplexing low coherence and high coherence interferometric system, which includes a Fizeau interferometer as the sensing element and a Michelson interferometer as the demodulating element, is designed for remote and high precision step height measurement. The Fizeau interferometer is placed in the remote field for sensing the measurand, while the Michelson interferometer which works in both modes of low coherence interferometry and high coherence interferometry is employed for demodulating the measurand. The range of the step height is determined by the low coherence interferometry and the value of it is measured precisely by the high coherence interferometry. High precision has been obtained by searching precisely the peak of the low coherence interferogram symmetrically from two sides of the low coherence interferogram and stabilizing the Michelson interferometer with a feedback loop. The maximum step height that could be measured is 6 mm while the measurement resolution is less than 1 nm. The standard deviation of 10 times measurement results of a step height of 1 mm configurated with two gauge blocks is 0.5 nm.

  19. Highly selective creation of hydrophilic micro-craters on super hydrophobic surface using electrohydrodynamic jet printing

    Science.gov (United States)

    Lee, Jaehyun; Hwang, Sangyeon; Prasetyo, Fariza Dian; Nguyen, Vu Dat; Hong, Jungwoo; Shin, Jennifer H.; Byun, Doyoung

    2014-11-01

    Selective surface modification is considered as an alternative to conventional printing techniques in high resolution patterning. Here, we present fabrication of hydrophilic patterns on the super hydrophobic surface, which makes structure on the hydrophilic region. The super hydrophobic surface is able to be chemically changed to hydrophilic with alcohols. As a consecutive process, electrohydrodynamic (EHD) jet printing was utilized to fabricate local hydrophilic craters with 30-200 μm sizes. 3 kinds of target liquids were deposited well on hydrophilic region; PEDOT (poly 3,4 ethylenediocythiophene), polystyrene nano-particles, and salmonella bacteria medium. Additionally, qualitative analysis were presented for modification mechanism and surface properties on super hydrophobic/hydrophilic by analysis of surface energy with contact angle, SEM (scanning electron microscopy) image, and SIMS (secondary ion mass spectroscopy) analysis. This new simple modification method provides possibility to be utilizing in bio-patterning engineering such as cell culturing microchip and lab on a chip. This research was supported by the Basi Science Research Program through the National Research Foundation of Korea (NRF) (Grand Number: 2014-023284).

  20. An optical fiber multiplexing interferometric system for measuring remote and high precision step height

    Science.gov (United States)

    Wang, Yunzhi; Xie, Fang; Ma, Sen; Chen, Liang

    2015-02-01

    In this paper, an optical fiber multiplexing interferometric system including a Fizeau interferometer and a Michelson interferometer is designed for remote and high precision step height measurement. The Fizeau interferometer which is inserted in the remote sensing field is used for sensing the measurand, while the Michelson interferometer which is stabilized by a feedback loop works in both modes of low coherence interferometry and high coherence interferometry to demodulate the measurand. The range of the step height is determined by the low coherence interferometry and the value of it is measured precisely by the high coherence interferometry. High precision has been obtained by using the symmetrical peak-searching method to address the peak of the low coherence interferogram precisely and stabilizing the Michelson interferometer with a feedback loop. The maximum step height that could be measured is 6 mm while the measurement resolution is less than 1 nm. The standard deviation of 10 times measurement results of a step height of 1 mm configurated with two gauge blocks is 0.5 nm.

  1. Multiplex cytokine profiling with highly pathogenic material: use of formalin solution in luminex analysis.

    Science.gov (United States)

    Dowall, Stuart D; Graham, Victoria A; Tipton, Thomas R W; Hewson, Roger

    2009-08-31

    Work with highly pathogenic material mandates the use of biological containment facilities, involving microbiological safety cabinets and specialist laboratory engineering structures typified by containment level 3 (CL3) and CL4 laboratories. Consequences of working in high containment are the practical difficulties associated with containing specialist assays and equipment often essential for experimental analyses. In an era of increased interest in biodefence pathogens and emerging diseases, immunological analysis has developed rapidly alongside traditional techniques in virology and molecular biology. For example, in order to maximise the use of small sample volumes, multiplexing has become a more popular and widespread approach to quantify multiple analytes simultaneously, such as cytokines and chemokines. The luminex microsphere system allows for the detection of many cytokines and chemokines in a single sample, but the detection method of using aligned lasers and fluidics means that samples often have to be analysed in low containment facilities. In order to perform cytokine analysis in materials from high containment (CL3 and CL4 laboratories), we have developed an appropriate inactivation methodology after staining steps, which although results in a reduction of median fluorescent intensity, produces statistically comparable outcomes when judged against non-inactivated samples. This methodology thus extends the use of luminex technology for material that contains highly pathogenic biological agents.

  2. On the High Temperature Deformation Behaviour of 2507 Super Duplex Stainless Steel

    Science.gov (United States)

    Mishra, M. K.; Balasundar, I.; Rao, A. G.; Kashyap, B. P.; Prabhu, N.

    2017-01-01

    High temperature deformation behaviour of 2507 super duplex stainless steel was investigated by conducting isothermal hot compression tests. The dominant restoration processes in ferrite and austenite phases present in the material were found to be distinct. The possible causes for these differences are discussed. Based on the dynamic materials model, processing map was developed to identify the optimum processing parameters. The microstructural mechanisms operating in the material were identified. A unified strain-compensated constitutive equation was established to describe the high temperature deformation behaviour of the material under the identified processing conditions. Standard statistical parameter such as correlation coefficient has been used to validate the established equation.

  3. On the High Temperature Deformation Behaviour of 2507 Super Duplex Stainless Steel

    Science.gov (United States)

    Mishra, M. K.; Balasundar, I.; Rao, A. G.; Kashyap, B. P.; Prabhu, N.

    2017-02-01

    High temperature deformation behaviour of 2507 super duplex stainless steel was investigated by conducting isothermal hot compression tests. The dominant restoration processes in ferrite and austenite phases present in the material were found to be distinct. The possible causes for these differences are discussed. Based on the dynamic materials model, processing map was developed to identify the optimum processing parameters. The microstructural mechanisms operating in the material were identified. A unified strain-compensated constitutive equation was established to describe the high temperature deformation behaviour of the material under the identified processing conditions. Standard statistical parameter such as correlation coefficient has been used to validate the established equation.

  4. PrimerSuite: A High-Throughput Web-Based Primer Design Program for Multiplex Bisulfite PCR.

    Science.gov (United States)

    Lu, Jennifer; Johnston, Andrew; Berichon, Philippe; Ru, Ke-Lin; Korbie, Darren; Trau, Matt

    2017-01-24

    The analysis of DNA methylation at CpG dinucleotides has become a major research focus due to its regulatory role in numerous biological processes, but the requisite need for assays which amplify bisulfite-converted DNA represents a major bottleneck due to the unique design constraints imposed on bisulfite-PCR primers. Moreover, a review of the literature indicated no available software solutions which accommodated both high-throughput primer design, support for multiplex amplification assays, and primer-dimer prediction. In response, the tri-modular software package PrimerSuite was developed to support bisulfite multiplex PCR applications. This software was constructed to (i) design bisulfite primers against multiple regions simultaneously (PrimerSuite), (ii) screen for primer-primer dimerizing artefacts (PrimerDimer), and (iii) support multiplex PCR assays (PrimerPlex). Moreover, a major focus in the development of this software package was the emphasis on extensive empirical validation, and over 1300 unique primer pairs have been successfully designed and screened, with over 94% of them producing amplicons of the expected size, and an average mapping efficiency of 93% when screened using bisulfite multiplex resequencing. The potential use of the software in other bisulfite-based applications such as methylation-specific PCR is under consideration for future updates. This resource is freely available for use at PrimerSuite website (www.primer-suite.com).

  5. A high-speed multiplexer-based fine-grain pipelined architecture for digital fuzzy logic controllers

    Science.gov (United States)

    Rashidi, Bahram; Masoud Sayedi, Sayed

    2015-12-01

    Design and implementation of a high-speed multiplexer-based fine-grain pipelined architecture for a general digital fuzzy logic controller has been presented. All the operators have been designed at gate level. For the multiplication, a multiplexer-based modified Wallace tree multiplier has been designed, and for the division and addition multiplexer-based non-restoring parallel divider and multiplexer-based Manchester adder have been used, respectively. To further increase the processing speed, fine-grain pipelining technique has been employed. By using this technique, the critical path of the circuit is broken into finer pieces. Based on the proposed architecture, and by using Quartus II 9.1, a sample two-input, one-output digital fuzzy logic controller with eight rules has been successfully synthesised and implemented on Stratix II field programmable gate array. Simulations were carried out using DSP Builder in the MATLAB/Simulink tool at a maximum clock rate of 301.84 MHz.

  6. PrimerSuite: A High-Throughput Web-Based Primer Design Program for Multiplex Bisulfite PCR

    Science.gov (United States)

    Lu, Jennifer; Johnston, Andrew; Berichon, Philippe; Ru, Ke-lin; Korbie, Darren; Trau, Matt

    2017-01-01

    The analysis of DNA methylation at CpG dinucleotides has become a major research focus due to its regulatory role in numerous biological processes, but the requisite need for assays which amplify bisulfite-converted DNA represents a major bottleneck due to the unique design constraints imposed on bisulfite-PCR primers. Moreover, a review of the literature indicated no available software solutions which accommodated both high-throughput primer design, support for multiplex amplification assays, and primer-dimer prediction. In response, the tri-modular software package PrimerSuite was developed to support bisulfite multiplex PCR applications. This software was constructed to (i) design bisulfite primers against multiple regions simultaneously (PrimerSuite), (ii) screen for primer-primer dimerizing artefacts (PrimerDimer), and (iii) support multiplex PCR assays (PrimerPlex). Moreover, a major focus in the development of this software package was the emphasis on extensive empirical validation, and over 1300 unique primer pairs have been successfully designed and screened, with over 94% of them producing amplicons of the expected size, and an average mapping efficiency of 93% when screened using bisulfite multiplex resequencing. The potential use of the software in other bisulfite-based applications such as methylation-specific PCR is under consideration for future updates. This resource is freely available for use at PrimerSuite website (www.primer-suite.com). PMID:28117430

  7. Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry.

    Science.gov (United States)

    Gilbert, Daniel F; Wilson, John C; Nink, Virginia; Lynch, Joseph W; Osborne, Geoffrey W

    2009-05-01

    Flow cytometry is an important drug discovery tool because it permits high-content multiparameter analysis of individual cells. A new method dramatically enhanced screening throughput by multiplexing many discrete fixed cell populations; however, this method is not suited to assays requiring functional cellular responses. HEK293 cells were transfected with unique mutant glycine receptors. Mutant receptor expression was confirmed by coexpression of yellow fluorescent protein (YFP). Commercially available cell-permeant dyes were used to label each glycine receptor expressing mutant with a unique optical code. All encoded cell lines were combined in a single tube and analyzed on a flow cytometer simultaneously before and after the addition of glycine receptor agonist. We decoded multiplexed cells that expressed functionally distinct glycine receptor chloride channels and analyzed responses to glycine in terms of chloride-sensitive YFP expression. Here, data provided by flow cytometry can be used to discriminate between functional and nonfunctional mutations in the glycine receptor, a process accelerated by the use of multiplexing. Further, this data correlates to data generated using a microscopy-based technique. The present study demonstrates multiplexed labeling of live cells, to enable cell populations to be subject to further cell culture and experimentation, and compares the results with those obtained using live cell microscopy. (c) 2009 International Society for Advancement of Cytometry.

  8. A highly accurate wireless digital sun sensor based on profile detecting and detector multiplexing technologies

    Science.gov (United States)

    Wei, Minsong; Xing, Fei; You, Zheng

    2017-01-01

    The advancing growth of micro- and nano-satellites requires miniaturized sun sensors which could be conveniently applied in the attitude determination subsystem. In this work, a profile detecting technology based high accurate wireless digital sun sensor was proposed, which could transform a two-dimensional image into two-linear profile output so that it can realize a high update rate under a very low power consumption. A multiple spots recovery approach with an asymmetric mask pattern design principle was introduced to fit the multiplexing image detector method for accuracy improvement of the sun sensor within a large Field of View (FOV). A FOV determination principle based on the concept of FOV region was also proposed to facilitate both sub-FOV analysis and the whole FOV determination. A RF MCU, together with solar cells, was utilized to achieve the wireless and self-powered functionality. The prototype of the sun sensor is approximately 10 times lower in size and weight compared with the conventional digital sun sensor (DSS). Test results indicated that the accuracy of the prototype was 0.01° within a cone FOV of 100°. Such an autonomous DSS could be equipped flexibly on a micro- or nano-satellite, especially for highly accurate remote sensing applications.

  9. Zero-voltage switching converter absorbing parasitic parameters for super high frequency induction heating

    Institute of Scientific and Technical Information of China (English)

    Zheng-shi WANG; Hui-ming CHEN

    2008-01-01

    This paper presents a novel mega-Hz-level super high frequency zero-voltage soft-switching converter for induction heating power supplies. The prominent advantage of this topology is that it can absorb both inductive and capacitive parasitic components in the converter. The switch devices operate in a zero-voltage soft-switching mode. Consequently, the high voltage and high current spikes caused by parasitic inductors or capacitors oscillation do not occur in this circuit, and the high power loss caused by high frequency switching can be greatly reduced. A large value inductor is adopted between the input capacitor and the switches, thus, this novel converter shares the benefits of both voltage-type and current-type circuits simultaneously, and there are no needs of dead time between two switches. The working principles in different modes are introduced, Results of simulation and experiments operated at around 1 MHz frequency verify the validity of parasitic components absorption and show that this converter is competent for super high frequency applications.

  10. Study on Plant Morphological Traits and Production Characteristics of Super High-Yielding Soybean

    Institute of Scientific and Technical Information of China (English)

    AO Xue; XIE Fu-ti; HAN Xiao-ri; ZHAO Ming-hui; ZHU Qian; LI Jie; ZHANG Hui-jun; WANG Hai-ying; YU Cui-mei; LI Chun-hong; YAO Xing-dong

    2013-01-01

    Super high-yielding soybean cultivar Liaodou 14, soybean cultivars from Ohio in the United States, and the common soybean cultivars from Liaoning Province, China, with similar geographic latitudes and identical pod-bearing habits were used as the study materials for a comparison of morphological traits and production characteristics to provide a theoretical basis for the breeding of improved super high-yielding soybean cultivars. Using a randomized block design, different soybean cultivars from the same latitude were compared under conventional and unconventional treatments for their production characteristics, including morphological traits, leaf area index (LAI), net photosynthesis rate, and dry matter accumulation. The specific characteristics of the super high-yielding soybean cultivar Liaodou 14 were analyzed. The results showed that the plant height of Liaodou 14 was significantly lower than that of the common cultivars from Liaoning, whereas the number of its main-stem nodes was higher than that of the cultivars from Ohio or Liaoning. A high pod density was observed in Liaodou 14 under conventional treatments. Under both conventional and unconventional treatments, the branch number of Liaodou 14 was markedly higher than that of the common cultivars from Liaoning, and its branch length and leaf inclination angle were significantly higher than those of common cultivars from Liaoning or Ohio. Only small changes in the leaf inclination angle were observed in Liaodou 14 treated with conventional or unconventional methods. Under each treatment, Liaodou 14 exhibited the lowest amplitude of reduction in SPAD values and net photosynthesis rates from the grain-filling to ripening stages;the cultivars from Ohio and the common cultivars from Liaoning exhibited more significant reductions. Liaodou 14 reached its peak LAI later than the other cultivars but maintained its LAI at a higher level for a longer duration. Under both conventional and unconventional treatments

  11. Optoelectronics for high-speed, wavelength-division- multiplexed optical communications networks

    Science.gov (United States)

    Hudgings, Janice Anne

    1999-11-01

    All-optical wavelength division multiplexed (WDM) communications networks have the potential to revolutionize future high-speed communications. All- optical networks offer enormous bandwidth capacity on the order of 25 THz, which is orders of magnitude greater than the capacity of radio or copper coaxial cable. In addition, wavelength division multiplexing offers a natural method of supporting with a single network multiple simultaneous applications with widely varying bandwidth requirements, modulation formats, and quality of service requirements. However, future implementation of all-optical WDM networks relies on further development of optical device technology. We present an experimental demonstration of a step- tunable, all-optical wavelength converter using cavity- enhanced four wave mixing (FWM) in a semiconductor laser. The converter is modulation format transparent, has a high conversion efficiency (> -10 dB), and is step- tunable over a large conversion range (> 2.5 THz). In addition, we derive a mathematical model of the wavelength converter, which is used to simulate step- tuning and to examine the relationship between the conversion efficiency and information bandwidth of the device. Likewise, the development of appropriate optical sources is critical to the success of all-optical communications networks. Vertical-cavity surface-emitting lasers (VCSELs) are rapidly emerging as strong competitors to the conventional edge-emitting semiconductor lasers. We have developed a novel three-contact VCSEL with an intracavity quantum-well absorber. The three-contact design of this structure enables independent control of the gain and absorber regions. If the VCSEL is designed such that the lasing wavelength is shorter than the bandedge of the intracavity absorber, the laser exhibits broad negative differential resistance, due to changes in the cavity photon density as the relative absorption is varied. With this design, the device is useful as an integrated

  12. High performance guard ring P-i-N photodetectors for microwave subcarrier wavelength division multiplexed networks

    Science.gov (United States)

    Odubanjo, Babatunde A.

    This dissertation is concerned with the design and fabrication of high performance guard ring photodetectors suitable for use in very dense WDM microwave subcarrier multiplexed (SCM) networks and Passive Optical Networks (PONs). Two issues are addressed. The first obvious quantity of concern is the problem of nonlinearity distortion characteristics of the detector arising from- internal electric field perturbation due to space charge and external loading induced effects-intermodulation effects due to the mixing between different microwave subcarriers-and optical beat notes interference issue. The second is the problem of slower responses due to diffusion currents. Our investigation begins with an analytic study of the transient response of a typical p-i-n and avalanche photodetectors (APDs) to determine the origin of their nonlinearities and slow response and their suitability for use in a narrow-band receiver required for SCM networks. We found that for a single channel link where the total receiver photocurrent is of the same magnitude as the signal photocurrent, avalanche photodetectors can dramatically increase receiver sensitivity. However in microwave subcarrier multiplexed networks the photodiodes in each receiver detect all subcarrier channels therefore the total received photocurrent is greater than that of a single channel by many orders. Thus under these criteria, APDs cannot provide any improvement in the receiver sensitivity. In fact avalanche gain noise mechanism degrades the expected S/N ratio due to its intrinsic excess noise factor. Furthermore, APDs typically require large bias voltage. There is also the problem of limited gain-bandwidth product which makes avalanche photodetectors unsuitable for very dense WDM networks. The clear choice for SCM-based networks is therefore the p-i-n photodiode which is shot-noise limited in high speed applications. We use our analysis to optimize a typical p-i-n photodiode. The result is a novel guard ring

  13. High-Speed Microscale Optical Tracking Using Digital Frequency-Domain Multiplexing.

    Science.gov (United States)

    Maclachlan, Robert A; Riviere, Cameron N

    2009-06-01

    Position-sensitive detectors (PSDs), or lateral-effect photodiodes, are commonly used for high-speed, high-resolution optical position measurement. This paper describes the instrument design for multidimensional position and orientation measurement based on the simultaneous position measurement of multiple modulated sources using frequency-domain-multiplexed (FDM) PSDs. The important advantages of this optical configuration in comparison with laser/mirror combinations are that it has a large angular measurement range and allows the use of a probe that is small in comparison with the measurement volume. We review PSD characteristics and quantitative resolution limits, consider the lock-in amplifier measurement system as a communication link, discuss the application of FDM to PSDs, and make comparisons with time-domain techniques. We consider the phase-sensitive detector as a multirate DSP problem, explore parallels with Fourier spectral estimation and filter banks, discuss how to choose the modulation frequencies and sample rates that maximize channel isolation under design constraints, and describe efficient digital implementation. We also discuss hardware design considerations, sensor calibration, probe construction and calibration, and 3-D measurement by triangulation using two sensors. As an example, we characterize the resolution, speed, and accuracy of an instrument that measures the position and orientation of a 10 mm × 5 mm probe in 5 degrees of freedom (DOF) over a 30-mm cube with 4-μm peak-to-peak resolution at 1-kHz sampling.

  14. High sensitivity automated multiplexed immunoassays using photonic crystal enhanced fluorescence microfluidic system.

    Science.gov (United States)

    Tan, Yafang; Tang, Tiantian; Xu, Haisheng; Zhu, Chenqi; Cunningham, Brian T

    2015-11-15

    We demonstrate a platform that integrates photonic crystal enhanced fluorescence (PCEF) detection of a surface-based microspot fluorescent assay with a microfluidic cartridge to achieve simultaneous goals of high analytic sensitivity (single digit pg/mL), high selectivity, low sample volume, and assay automation. The PC surface, designed to provide optical resonances for the excitation wavelength and emission wavelength of Cyanines 5 (Cy5), was used to amplify the fluorescence signal intensity measured from a multiplexed biomarker microarray. The assay system is comprised of a plastic microfluidic cartridge for holding the PC and an assay automation system that provides a leak-free fluid interface during introduction of a sequence of fluids under computer control. Through the use of the assay automation system and the PC embedded within the microfluidic cartridge, we demonstrate pg/mL-level limits of detection by performing representative biomarker assays for interleukin 3 (IL3) and Tumor Necrosis Factor (TNF-α). The results are consistent with limits of detection achieved without the use of the microfluidic device with the exception that coefficients of variability from spot-to-spot are substantially lower than those obtained by performing assays with manual manipulation of assay liquids. The system's capabilities are compatible with the goal of diagnostic instruments for point-of-care settings.

  15. Single particle tracking through highly scattering media with multiplexed two-photon excitation

    Science.gov (United States)

    Perillo, Evan; Liu, Yen-Liang; Liu, Cong; Yeh, Hsin-Chih; Dunn, Andrew K.

    2015-03-01

    3D single-particle tracking (SPT) has been a pivotal tool to furthering our understanding of dynamic cellular processes in complex biological systems, with a molecular localization accuracy (10-100 nm) often better than the diffraction limit of light. However, current SPT techniques utilize either CCDs or a confocal detection scheme which not only suffer from poor temporal resolution but also limit tracking to a depth less than one scattering mean free path in the sample (typically validated our microscope by tracking (1) fluorescent nanoparticles in a prescribed motion inside gelatin gel (with 1% intralipid) and (2) labeled single EGFR complexes inside skin cancer spheroids (at least 8 layers of cells thick) for ~10 minutes. Furthermore we discuss future capabilities of our multiplexed two-photon microscope design, specifically to the extension of (1) simultaneous multicolor tracking (i.e. spatiotemporal co-localization analysis) and (2) FRET studies (i.e. lifetime analysis). The high resolution, high depth penetration, and multicolor features of this microscope make it well poised to study a variety of molecular scale dynamics in the cell, especially related to cellular trafficking studies with in vitro tumor models and in vivo.

  16. Super-Resolution Imaging by Arrays of High-Index Spheres Embedded in Transparent Matrices

    CERN Document Server

    Allen, Kenneth W; Li, Yangcheng; Limberopoulos, Nicholaos I; Walker, Dennis E; Urbas, Augustine M; Astratov, Vasily N

    2014-01-01

    We fabricated thin-films made from polydimethylsiloxane (PDMS) with embedded high-index (n~1.9-2.2) microspheres for super-resolution imaging applications. To control the position of microspheres, such films can be translated along the surface of the nanoplasmonic structure to be imaged. Microsphere-assisted imaging, through these matrices, provided lateral resolution of ~{\\lambda}/7 in nanoplasmonic dimer arrays with an illuminating wavelength {\\lambda}=405 nm. Such thin films can be used as contact optical components to boost the resolution capability of conventional microscopes.

  17. Microstructures and constituents of super-high strength aluminum alloy ingots made through LFEC process

    Directory of Open Access Journals (Sweden)

    WANG Shuang

    2007-11-01

    Full Text Available Ingots of a new super-high strength Al-Zn-Mg-Cu-Zr alloy were produced respectively by low frequency electromagnetic casting (LFEC and by conventional direct chill (DC casting process. Microstructure and constituents of the ingots were studied. The results indicated that the LFEC process significantly refines microstructure and constituents of the alloy, and to some extent, decreases the area (or volume fraction of constituents and eutectic structure precipitated at grain boundaries. But, no difference in the type of constituents was observed between LFEC and DC ingots. The results also showed LFEC process can improve the as-cast mechanical properties.

  18. Stabilization of high and low solids Consolidated Incinerator Facility (CIF) waste with super cement

    Energy Technology Data Exchange (ETDEWEB)

    Walker, B.W.

    2000-01-11

    This report details solidification activities using selected Mixed Waste Focus Area technologies with the High and Low Solid waste streams. Ceramicrete and Super Cement technologies were chosen as the best possible replacement solidification candidates for the waste streams generated by the SRS incinerator from a list of several suggested Mixed Waste Focus Area technologies. These technologies were tested, evaluated, and compared to the current Portland cement technology being employed. Recommendation of a technology for replacement depends on waste form performance, process flexibility, process complexity, and cost of equipment and/or raw materials.

  19. Proposal to detect an emission of unusual super-high energy electrons in electron storage rings

    Directory of Open Access Journals (Sweden)

    Da-peng Qian

    2014-01-01

    Full Text Available According to an extended Lorentz–Einstein mass formula taken into the uncertainty principle, it is predicted that the electron beams passing accelerating electric field should with a small probability generate abnormal super-high energy electrons which are much higher than the beam energy. Author’s preliminary experiment result at electron storage ring has hinted these signs, so suggests to more strictly detect this unusual phenomenon, and thus to test the extended mass formula as well as a more perfect special relativity.

  20. Development of the super high angular resolution principle for X-ray imaging *

    Institute of Scientific and Technical Information of China (English)

    Chen Zhang; Shuang-Nan Zhang

    2011-01-01

    Development of the Super High Angular Resolution Principle (SHARP)for coded-mask X-ray imaging is presented. We prove that SHARP can be considered as a generalized coded mask imaging method with a coding pattern comprised of diffraction-interference fringes in the mask pattern. The angular resolution of SHARP can be improved by detecting the fringes more precisely than the mask's element size,i.e. by using a detector with a pixel size smaller than the mask's element size. The proposed mission SHARP-X for solar X-ray observations is also briefly discussed.

  1. A Super High Resolution Distance Measurement Method Based on Phase Comparison

    Institute of Scientific and Technical Information of China (English)

    LI Zhi-Qi; ZHOU Wei; MIAO Miao; ZHOU Hui; ZHENG Sheng-Feng

    2008-01-01

    @@ Phase comparison method can enhance the measurement resolution to 10-13/τ in time domain. This method can also be used in distance measurement in the navigation and positioning. We propose a super high-resolution distance measurement based on linear phase comparison method. A high resolution scheme is put forward on the basis of the research of major factors concerning the phase comparison in the distance measurement. Conversion of a high-linearity phase difference to voltage and high-resolution voltage meter make it possible to obtain a very high phase measurement resolution. When the purpose is to measure distance, the phase noise of frequency source used in the measurement can be reduced partly. Thus this method is favourable for high resolution distance measurement. The precision of the distance measurement can reach 0.1c ps with c being the velocity of light in vacuum.

  2. Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model

    Institute of Scientific and Technical Information of China (English)

    Florian Graepler; Ulrike A Lauer; Reinhard Vonthein; Michael Gregor; Sorin Armeanu; Michael Bitzer; Ulrich M. Lauer; Marie-Luise Lemken; Wolfgang A Wybranietz; Ulrike Schmidt; Irina Smirnow; Christine D Groβ; Martin Spiegel; Andrea Schenk; Hansj(o)rg Graf

    2005-01-01

    AIM: To investigate the effects of catalytically superior gene-directed enzyme prodrug therapy systems on a rat hepatoma model.METHODS: To increase hepatoma cell chemosensitivity for the prodrug 5-fluorocytosine (5-FC), we generated a chimeric bifunctional SuperCD suicide gene, a fusion of the yeast cytosine deaminase (YCD) and the yeast uracil phosphoribosyltransferase (YUPRT) gene.RESULTS: In vitro stably transduced Morris rat hepatoma cells (MH) expressing the bifunctional SuperCD suicide gene (MH SuperCD) showed a clearly marked enhancement in cell killing when incubated with 5-FC as compared with MH ceils stably expressing YCD solely (MH YCD) or the cytosine deaminase gene of bacterial origin(MH BCD), respectively. In vivo, MH SuperCD tumors implanted both subcutaneously as well as orthotopically into the livers of syngeneic ACI rats demonstrated significant tumor regressions (P<0.01) under both high dose as well as low dose systemic 5-FC application,whereas MH tumors without transgene expression (MH naive) showed rapid progression. For the first time, an order of in vivo suicide gene effectiveness (SuperCD>>YCD > > BCD > > > negative control) was defi ned as a result of a directin vivo comparison of all three suicide genes.CONCLUSION: Bifunctional SuperCD suicide gene expression is highly effective in a rat hepatoma model,thereby significantly improving both the therapeutic index and the efficacy of hepatocellular carcinoma killing by fluorocytosine.

  3. High throughput and multiplex localization of proteins and cells for in situ micropatterning using pneumatic microfluidics.

    Science.gov (United States)

    Wang, Jian-Chun; Liu, Wenming; Tu, Qin; Ma, Chao; Zhao, Lei; Wang, Yaolei; Ouyang, Jia; Pang, Long; Wang, Jinyi

    2015-02-07

    Micropatterning technologies are emerging as an enabling tool for various microfluidic-based applications in life sciences. However, the high throughput and multiplex localization of multiple bio-components in a microfluidic device has not yet been well established. In this paper, we describe a simple and in situ micropatterning method using an integrated microfluidic device with pneumatic microstructures (PμSs) for highly controllable immobilization of both proteins and cells in a high throughput, geometry-dynamic, and multi-patterning way. The precise Pluronic F127 passivation of a microchamber surface except the PμS-blocked regions was performed and characterized, and the spatial dynamics and consistency of both the PμSs and protein/cell micropatterning were optically evaluated and quantitatively demonstrated too. Furthermore, a systematic investigation of PμS-assisted micropatterning in microfluidics was carried out. The feature of high throughput and spatial control of micropatterning can be simply realized by using the well-designed PμS arrays. Meanwhile, the co-micropatterning of different proteins (bovine serum albumin and chicken egg albumin) and cells (human umbilical vein endothelial cells and human hepatocellular carcinoma cells) in a microfluidic device was successfully accomplished with the orderly serial manipulation of PμS groups. We demonstrate that PμS-assisted micropatterning can be applied as a convenient microfluidic component for large-scale and diversified protein/cell patterning and manipulation, which could be useful for cell-based tissue organization, high-throughput imaging, protein-related interactions and immunoassays.

  4. A novel multiplex cell viability assay for high-throughput RNAi screening.

    Science.gov (United States)

    Gilbert, Daniel F; Erdmann, Gerrit; Zhang, Xian; Fritzsche, Anja; Demir, Kubilay; Jaedicke, Andreas; Muehlenberg, Katja; Wanker, Erich E; Boutros, Michael

    2011-01-01

    Cell-based high-throughput RNAi screening has become a powerful research tool in addressing a variety of biological questions. In RNAi screening, one of the most commonly applied assay system is measuring the fitness of cells that is usually quantified using fluorescence, luminescence and absorption-based readouts. These methods, typically implemented and scaled to large-scale screening format, however often only yield limited information on the cell fitness phenotype due to evaluation of a single and indirect physiological indicator. To address this problem, we have established a cell fitness multiplexing assay which combines a biochemical approach and two fluorescence-based assaying methods. We applied this assay in a large-scale RNAi screening experiment with siRNA pools targeting the human kinome in different modified HEK293 cell lines. Subsequent analysis of ranked fitness phenotypes assessed by the different assaying methods revealed average phenotype intersections of 50.7±2.3%-58.7±14.4% when two indicators were combined and 40-48% when a third indicator was taken into account. From these observations we conclude that combination of multiple fitness measures may decrease false-positive rates and increases confidence for hit selection. Our robust experimental and analytical method improves the classical approach in terms of time, data comprehensiveness and cost.

  5. Family of enhanced photoacoustic imaging agents for high-sensitivity and multiplexing studies in living mice.

    Science.gov (United States)

    de la Zerda, Adam; Bodapati, Sunil; Teed, Robert; May, Salomón Y; Tabakman, Scott M; Liu, Zhuang; Khuri-Yakub, Butrus T; Chen, Xiaoyuan; Dai, Hongjie; Gambhir, Sanjiv S

    2012-06-26

    Photoacoustic imaging is a unique modality that overcomes to a great extent the resolution and depth limitations of optical imaging while maintaining relatively high contrast. However, since many diseases will not manifest an endogenous photoacoustic contrast, it is essential to develop exogenous photoacoustic contrast agents that can target diseased tissue(s). Here we present a family of novel photoacoustic contrast agents that are based on the binding of small optical dyes to single-walled carbon nanotubes (SWNT-dye). We synthesized five different SWNT-dye contrast agents using different optical dyes, creating five "flavors" of SWNT-dye nanoparticles. In particular, SWNTs that were coated with either QSY(21) (SWNT-QSY) or indocyanine green (SWNT-ICG) exhibited over 100-times higher photoacoustic contrast in living animals compared to plain SWNTs, leading to subnanomolar sensitivities. We then conjugated the SWNT-dye conjugates with cyclic Arg-Gly-Asp peptides to molecularly target the α(v)β(3) integrin, which is associated with tumor angiogenesis. Intravenous administration of these tumor-targeted imaging agents to tumor-bearing mice showed significantly higher photoacoustic signal in the tumor than in mice injected with the untargeted contrast agent. Finally, we were able to spectrally separate the photoacoustic signals of SWNT-QSY and SWNT-ICG in living animals injected subcutaneously with both particles in the same location, opening the possibility for multiplexing in vivo studies.

  6. Lanthanide-containing polymer microspheres by multiple-stage dispersion polymerization for highly multiplexed bioassays.

    Science.gov (United States)

    Abdelrahman, Ahmed I; Dai, Sheng; Thickett, Stuart C; Ornatsky, Olga; Bandura, Dmitry; Baranov, Vladimir; Winnik, Mitchell A

    2009-10-28

    We describe the synthesis and characterization of metal-encoded polystyrene microspheres by multiple-stage dispersion polymerization with diameters on the order of 2 mum and a very narrow size distribution. Different lanthanides were loaded into these microspheres through the addition of a mixture of lanthanide salts (LnCl(3)) and excess acrylic acid (AA) or acetoacetylethyl methacrylate (AAEM) dissolved in ethanol to the reaction after about 10% conversion of styrene, that is, well after the particle nucleation stage was complete. Individual microspheres contain ca. 10(6)-10(8) chelated lanthanide ions, of either a single element or a mixture of elements. These microspheres were characterized one-by-one utilizing a novel mass cytometer with an inductively coupled plasma (ICP) ionization source and time-of-flight (TOF) mass spectrometry detection. Microspheres containing a range of different metals at different levels of concentration were synthesized to meet the requirements of binary encoding and enumeration encoding protocols. With four different metals at five levels of concentration, we could achieve a variability of 624, and the strategy we report should allow one to obtain much larger variability. To demonstrate the usefulness of element-encoded beads for highly multiplexed immunoassays, we carried out a proof-of-principle model bioassay involving conjugation of mouse IgG to the surface of La and Tm containing particles and its detection by an antimouse IgG bearing a metal-chelating polymer with Pr.

  7. A novel multiplex cell viability assay for high-throughput RNAi screening.

    Directory of Open Access Journals (Sweden)

    Daniel F Gilbert

    Full Text Available Cell-based high-throughput RNAi screening has become a powerful research tool in addressing a variety of biological questions. In RNAi screening, one of the most commonly applied assay system is measuring the fitness of cells that is usually quantified using fluorescence, luminescence and absorption-based readouts. These methods, typically implemented and scaled to large-scale screening format, however often only yield limited information on the cell fitness phenotype due to evaluation of a single and indirect physiological indicator. To address this problem, we have established a cell fitness multiplexing assay which combines a biochemical approach and two fluorescence-based assaying methods. We applied this assay in a large-scale RNAi screening experiment with siRNA pools targeting the human kinome in different modified HEK293 cell lines. Subsequent analysis of ranked fitness phenotypes assessed by the different assaying methods revealed average phenotype intersections of 50.7±2.3%-58.7±14.4% when two indicators were combined and 40-48% when a third indicator was taken into account. From these observations we conclude that combination of multiple fitness measures may decrease false-positive rates and increases confidence for hit selection. Our robust experimental and analytical method improves the classical approach in terms of time, data comprehensiveness and cost.

  8. The high-energy environment in the super-earth system CoRoT-7

    CERN Document Server

    Poppenhaeger, K; Schröter, S; Lalitha, S; Kashyap, V; Schmitt, J H M M

    2012-01-01

    High-energy irradiation of exoplanets has been identified to be a key influence on the stability of these planets' atmospheres. So far, irradiation-driven mass-loss has been observed only in two Hot Jupiters, and the observational data remain even more sparse in the super-earth regime. We present an investigation of the high-energy emission in the CoRoT-7 system, which hosts the first known transiting super-earth. To characterize the high-energy XUV radiation field into which the rocky planets CoRoT-7b and CoRoT-7c are immersed, we analyzed a 25 ks XMM-Newton observation of the host star. Our analysis yields the first clear (3.5 sigma) X-ray detection of CoRoT-7. We determine a coronal temperature of ca. 3 MK and an X-ray luminosity of 3*10^28 erg/s. The level of XUV irradiation on CoRoT-7b amounts to ca. 37000 erg/cm^2/s. Current theories for planetary evaporation can only provide an order-of-magnitude estimate for the planetary mass loss; assuming that CoRoT-7b has formed as a rocky planet, we estimate that...

  9. Use of subcarrier multiplexing for self-routing of data packets in a high-performance system area network

    Science.gov (United States)

    Saraswat, Sanjay

    1998-10-01

    In self-routing packet networks, the state of intermediate nodes (switches) is set or reset on the basis of the information present in the packet header. Subcarrier multiplexing (SCM) modulates a number of frequency-separated RF sub-carriers onto a common laser at a single wavelength. SCM has the advantage of high data throughput. It also requires fewer opto-electronic components and avoids walk- off between header and payload due to fiber dispersion. In this paper we describe a novel use of sub-carrier multiplexing for self-routing of data packet within the switching fabric of a high performance system area network. Using SCM data packets are routed optically to the destination without being converted to the electrical domain at the intermediate stages within the network.

  10. Fuel rod behavior under normal operating conditions in Super Fast Reactor with high power density

    Energy Technology Data Exchange (ETDEWEB)

    Ju, Haitao, E-mail: haitaoju@gmail.com [Science and Technology on Reactor System Design Technology Laboratory, Chengdu, Sichuan 610041 (China); Ishiwatari, Yuki [Department of Nuclear Engineering and Management, The University of Tokyo, Hongo, Bunkyo, Tokyo 113-8656 (Japan); Oka, Yoshiaki [Joint Department of Nuclear Energy, Waseda University, Totsukamachi, Shinjuku, Tokyo 169-8050 (Japan)

    2015-08-15

    Highlights: • The improved core of Super Fast Reactor with high power density is analyzed. • We analyzed four types of the limiting fuel rods. • The influence of Pu enrichment and compressive stress to yield strength ratio are analyzed. • The improved fuel rod design of the new core is suggested. - Abstract: A Super Fast Reactor is a pressure-vessel type, fast spectrum SuperCritical Water Reactor (SCWR) which is presently researched in a Japanese project. A preliminary core has an average power density of 158.8 W/cc. However one of the most important advantages of the Super Fast Reactor is the higher power density compared to the thermal spectrum SCWR, which reduces the capital cost. After the sensitivity analyses on the fuel rod configurations, the fuel assembly configurations and the core configurations, an improved core with an average power density of 294.8 W/cc is designed by 3-D neutronic/thermal-hydraulic coupled calculations. In order to ensure the fuel rod integrity of new core design with high power density, the fuel rod behaviors under normal operating condition are analyzed using fuel performance code FEMAXI-6. The power histories of each fuel rod are taken from the neutronics calculation results in the core design. The cladding surface temperature histories are generated from the thermal-hydraulic calculation results in the core design. Four types of the limiting fuel rods, individually with the Maximum Cladding Surface Temperature (MCST), Maximum Power Peak (MPP), Maximum Discharge Burnup (MDB) and Different Coolant Flow Pattern (DCFP), are chosen to cover all the fuel rods in the core. The available design range of the fuel rod design parameters, such as initial gas plenum pressure, gas plenum position, gas plenum length, grain size and gap size, are found out in order to satisfy the following design criteria: (1) Maximum fuel centerline temperature should be less than 1900 °C. (2) Maximum cladding stress in circumferential direction should

  11. Double domain wavelength multiplexed Fizeau interferometer with high resolution dynamic sensing and absolute length detection

    Science.gov (United States)

    Antonacci, Julián; Arenas, Gustavo F.; Duchowicz, Ricardo

    2017-04-01

    In this work, we present a simple photonic instrument that has the ability of measuring positions, distances and vibrations with very high resolution by means of two Fizeau interferometers (FI), both using the same optical fiber end as a probe tip itself. On the one hand we have a time domain FI powered with a 1310 nm laser and monitored by an InGaAs detector providing displacement information with resolution around a tenth of nm but regardless of the absolute position of object and of the displacement sense. On the other, a spectral domain FI version based on a super luminescent source (SLED) centred at 800 nm with bandwidth of nearly 40 nm is analysed in real time by means of a digital spectrometer. Each spectrum is acquired in a very small time interval and provides information of both length of the cavity as well as its correct sense of evolution. Resolution of this system is lower than its complementary temporal case, but distance and sense measurements are absolute and can be determined successfully by adequate processing of spectral signal.Both interferometers are optically coupled to a single fiber optic probe and are wavelength modulated.Therefore, combination of both sensors results in a new one which allows the correct knowledge of an object or surfaces under test, i.e. a high resolution of displacement data plus its absolute position and true sense of movement.

  12. Highly efficient volume hologram multiplexing in thick dye-doped jelly-like gelatin.

    Science.gov (United States)

    Katarkevich, Vasili M; Rubinov, Anatoli N; Efendiev, Terlan Sh

    2014-08-01

    Dye-doped jelly-like gelatin is a thick-layer self-developing photosensitive medium that allows single and multiplexed volume phase holograms to be successfully recorded using pulsed laser radiation. In this Letter, we present a method for multiplexed recording of volume holograms in a dye-doped jelly-like gelatin, which provides significant increase in their diffraction efficiency. The method is based on the recovery of the photobleached dye molecule concentration in the hologram recording zone of gel, thanks to molecule diffusion from other unexposed gel areas. As an example, an optical recording of a multiplexed hologram consisting of three superimposed Bragg gratings with mean values of the diffraction efficiency and angular selectivity of ∼75% and ∼21', respectively, is demonstrated by using the proposed method.

  13. Multiplexer-based architecture for high-density, low-power gate arrays

    Science.gov (United States)

    Landers, Robert J.; Mahant-Shetti, Shivaling S.; Lemonds, Carl

    1995-04-01

    This paper presents a novel architecture that provides higher density and lower power dissipation than conventional basecells. The layout of transistors in this small basecell allows the efficient construction of multiplexers with minimal use of programmable layers. The multiplexer can be used to create any 2 input and some 3 input functions in one basecell. Internal fanout, rather than typical output load, defines the size of driver and multiplexer transistors, which can be independently tailored for the desired speed/area/power target. This basecell, which is well suited for implementing datapath elements, has been used to create a 16 x 16-b multiplier operating at 50 MHz in 314-500 micron(exp 2) in 0.6 micron technology.

  14. Weldability of 780 MPa Super-High Strength Heavy-Duty Truck Crossbeam Steel

    Institute of Scientific and Technical Information of China (English)

    WANG Xiao-nan; DI Hong-shuang; ZHANG Chit; DU Lin-xiu; DONG Xue-xir

    2012-01-01

    CO2-shielded welding experiments of newly developed, 780 MPa super-high strength heavy-duty truck crossbeam steel were conducted, and the microstructure, microhardness, mechanical properties, and impact tough- hess of the welded joint were studied. The evolution of the microstructure of the welded joint occurred as follows.. welding seam (acicular ferrite+proeutectoid ferrite)→fusion zone (granular bainite-long strip M/A island)→coarse grain zone (granular bainite-long strip or short bar M/A island)→fine grain zone (ferrite+ pearlite+ blocky M/A is- land)→mixed grained zone (ferrite+granular bainite+blocky M/A island)→base metal (proeutectoid ferrite+gran- ular bainite-hlocky or granular M/A island). Increasing the density of the grain boundaries can effectively improve the impact toughness, and the blocky M/A island hindered crack propagation more effectively than the long strip M/A island. The new hot-rolled 780 MPa super-high strength steel had excellent weldability. The welding technology was applied under the following conditions: welding voltage was 20 to 21 V, welding current was 200 to 210 A, and the gas flow rate was 25 L/rain.

  15. Automated high multiplex qPCR platform for simultaneous detection and quantification of multiple nucleic acid targets.

    Science.gov (United States)

    Hlousek, Louis; Voronov, Sergey; Diankov, Vesselin; Leblang, Amy B; Wells, Patrick J; Ford, Donna M; Nolling, Jork; Hart, Kyle W; Espinoza, Patricio A; Bristol, Michael R; Tsongalis, Gregory J; Yen-Lieberman, Belinda; Slepnev, Vladimir I; Kong, Lilly I; Lee, Ming-Chou

    2012-05-01

    Quantitative PCR (qPCR) using real-time detection of amplification is limited to a small number of targets within a single reaction. The ICEPlex system, using our scalable target analysis routine (STAR) technology, was developed to provide an automated, high multiplexing PCR solution. ICEPlex combines PCR thermal cycling with dynamic, sequential amplicon separation by capillary electrophoresis and two-color quantitative detection in a single integrated system. In contrast to probe-based qPCR, ICEPlex directly measures amplicon accumulation through incorporation of labeled primers. Three orders of magnitude of optical detection range and at least 7 logs of detectable target concentration range are demonstrated. The system can separate more than 50 amplicons per color channel, ranging from 100 to 500 bases, providing broad multiplexing capabilities for a wide spectrum of nucleic acid amplification applications. ICEPlex can be used for analysis of viral DNA or RNA targets, detection of genetic variants, and for reverse-transcriptase PCR gene expression panels.

  16. Meshing Highly Regular Structures: The Case of Super Carbon Nanotubes of Arbitrary Order

    Directory of Open Access Journals (Sweden)

    Christian Schröppel

    2015-01-01

    Full Text Available Mesh generation is an important step in many numerical methods. We present the “Hierarchical Graph Meshing” (HGM method as a novel approach to mesh generation, based on algebraic graph theory. The HGM method can be used to systematically construct configurations exhibiting multiple hierarchies and complex symmetry characteristics. The hierarchical description of structures provided by the HGM method can be exploited to increase the efficiency of multiscale and multigrid methods. In this paper, the HGM method is employed for the systematic construction of super carbon nanotubes of arbitrary order, which present a pertinent example of structurally and geometrically complex, yet highly regular, structures. The HGM algorithm is computationally efficient and exhibits good scaling characteristics. In particular, it scales linearly for super carbon nanotube structures and is working much faster than geometry-based methods employing neighborhood search algorithms. Its modular character makes it conducive to automatization. For the generation of a mesh, the information about the geometry of the structure in a given configuration is added in a way that relates geometric symmetries to structural symmetries. The intrinsically hierarchic description of the resulting mesh greatly reduces the effort of determining mesh hierarchies for multigrid and multiscale applications and helps to exploit symmetry-related methods in the mechanical analysis of complex structures.

  17. Real time quantitative amplification detection on a microarray: towards high multiplex quantitative PCR.

    NARCIS (Netherlands)

    Pierik, A.; Moamfa, M; van Zelst, M.; Clout, D.; Stapert, H.; Dijksman, Johan Frederik; Broer, D.; Wimberger-Friedl, R.

    2012-01-01

    Quantitative real-time polymerase chain reaction (qrtPCR) is widely used as a research and diagnostic tool. Notwithstanding its many powerful features, the method is limited in the degree of multiplexing to about 6 due to spectral overlap of the available fluorophores. A new method is presented that

  18. High-Spatial-Multiplicity Multicore Fibers for Future Dense Space-Division-Multiplexing Systems

    DEFF Research Database (Denmark)

    Matsuo, Shoichiro; Takenaga, Katsuhiro; Sasaki, Yusuke;

    2016-01-01

    Multicore fibers and few-mode fibers have potential application in realizing dense-space-division multiplexing systems. However, there are some tradeoff requirements for designing the fibers. In this paper, the tradeoff requirements such as spatial channel count, crosstalk, differential mode dela...

  19. Multiplexing Fluo-4 NW and a GeneBLAzer transcriptional assay for high-throughput screening of G-protein-coupled receptors.

    Science.gov (United States)

    Hanson, Bonnie J

    2006-09-01

    Activation of G-protein-coupled receptors (GPCRs) leads to a cascade of signaling events, including calcium mobilization and downstream transcriptional activation of various proteins. Two commonly used methods of high-throughput screening for GPCRs include calcium-sensitive dyes, such as Fluo-4 NW, and reporter gene assays, such as beta-lactamase. To determine whether the advantages of each assay format could be combined by multiplexing, Jurkat and CHO-K1 cell lines over-expressing the M1 muscarinic receptor and beta-lactamase under control of an NFAT response element were tested in a multiplexed format. The Jurkat cell line was further screened with a subset of the LOPAC(1280) library. The multiplexing assay was compatible with both the CHO-K1 and Jurkat cell lines. For the screen, there was 100% correlation of on-target hits in the multiplexed format, and several false positives with each assay format were identified. Therefore, not only can the assays be multiplexed, but by multiplexing, the false positives associated with each assay format also could be easily identified. In addition to enhanced reliability, this method saves time and money because only half the amount of compounds, cells, and consumables are needed to screen a cell line in a multiplexed mode versus separate screening by both methods.

  20. Volume and structural analysis of super-cooled water under high pressure

    Science.gov (United States)

    Duki, Solomon F.; Tsige, Mesfin

    2012-02-01

    Motivated by recent experimental study of super-cooled water at high pressure [1], we performed atomistic molecular dynamic simulations study on bulk water molecules at isothermal-isobaric ensemble. These simulations are performed at temperatures that range from 40 K to 380 K using two different cooling rates, 10K/ns and 10K/5ns, and pressure that ranges from 1atm to 10000 atm. Our analysis for the variation of the volume of the bulk sample against temperature indicates a downward concave shape for pressures above certain values, as reported in [1]. The same downward concave behavior is observed at high pressure on the mean-squared-displacements (MSD) of the water molecules when the MSD is plotted against time. To get further insight on the effect of the pressure on the sample we have also performed a structural analysis of the sample.[4pt] [1] O. Mishima, J. Chem. Phys. 133, 144503 (2010);

  1. Multiplex immunoassay for persistent organic pollutants in tilapia: comparison of imaging- and flow cytometry-based platforms using spectrally encoded paramagnetic microspheres

    NARCIS (Netherlands)

    Meimaridou, A.; Haasnoot, W.; Shelver, W.L.; Franek, M.; Nielen, M.W.F.

    2013-01-01

    Recent developments in spectrally encoded microspheres (SEMs)-based technologies provide high multiplexing possibilities. Most SEMs-based assays require a flow cytometer with sophisticated fluidics and optics. A new imaging super-paramagnetic SEMs-based alternative platform transports SEMs with cons

  2. Navigability of multiplex temporal network

    Science.gov (United States)

    Wang, Yan; Song, Qiao-Zhen

    2017-01-01

    Real world complex systems have multiple levels of relationships and in many cases, they need to be modeled as multiplex networks where the same nodes can interact with each other in different layers, such as social networks. However, social relationships only appear at prescribed times so the temporal structures of edge activations can also affect the dynamical processes located above them. To consider both factors are simultaneously, we introduce multiplex temporal networks and propose three different walk strategies to investigate the concurrent dynamics of random walks and the temporal structure of multiplex networks. Thus, we derive analytical results for the multiplex centrality and coverage function in multiplex temporal networks. By comparing them with the numerical results, we show how the underlying topology of the layers and the walk strategy affect the efficiency when exploring the networks. In particular, the most interesting result is the emergence of a super-diffusion process, where the time scale of the multiplex is faster than that of both layers acting separately.

  3. Arrayed Waveguide Gratings and Their Application Using Super-High-Δ Silica-Based Planar Lightwave Circuit Technology

    Science.gov (United States)

    Maru, Koichi; Uetsuka, Hisato

    This paper reviews our recent progress on arrayed waveguide gratings (AWGs) using super-high-Δ silica-based planar lightwave circuit (PLC) technology and their application to integrated optical devices. Factors affecting the chip size of AWGs and the impact of increasing relative index difference Δ on the chip size are investigated, and the fabrication result of a compact athermal AWG using 2.5%-Δ silica-based waveguides is presented. As an application of super-high-Δ AWGs to integrated devices, a flat-passband multi/demultiplexer consisting of an AWG and cascaded MZIs is presented.

  4. Generation and transmission of 512-Gb/s quad-carrier digital super-Nyquist spectral shaped signal.

    Science.gov (United States)

    Zhang, Junwen; Yu, Jianjun; Chi, Nan

    2013-12-16

    A novel digital super-Nyquist signal generation scheme is proposed to further suppress the Nyquist signal bandwidth and reduce the channel crosstalk without using optical pre-filtering. The spectrum of the generated super-Nyquist 9-QAM signal is much more compact compared with regular Nyquist QPSK signal. Therefore, only optical couplers are needed for super-Nyquist WDM channel multiplexing. By using the 64-GSa/s high speed DAC, 32-GBaud super-Nyquist 9-QAM signal is generated within 25-GHz grid for quad-carrier 400G channels. We successfully generate and transmit 4 channels quad-carrier 512-Gb/s super-Nyquist 9-QAM-like signal within 100-GHz grid over 2975-km at a net SE of 4b/s/Hz (after excluding the 20% soft-decision FEC overhead).

  5. Type III Dyson Sphere of Highly Advanced Civilizations around a Super Massive Black Hole

    CERN Document Server

    Inoue, Makoto

    2011-01-01

    We describe a new system for a society of highly advanced civilizations around a super massive black hole (SMBH), as an advanced Type III "Dyson Sphere", pointing out an efficient usage of energy for the advanced civilizations. SMBH also works as a sink for waste materials. Here we assume that Type III civilisations of Kardashev classification [1] form a galactic club [2] in a galaxy, and the energy from the SMBH will be delivered to the club members, forming an energy control system similar to power grids in our present society. The energy is probably transmitted by a sharp beam with coherent electro-magnetic waves, which provide a new concept for the search for extraterrestrial intelligence (SETI) via detection of such energy transmission signals. This expands the search window for other intelligences within the Universe.

  6. Crucial technologies in High the design of Xiluodu Super Arch Dam

    Institute of Scientific and Technical Information of China (English)

    Wang Renkun

    2012-01-01

    Some super high arch dams ( SHADs), like Xiluodu Arch Dam, after their heights reaching the magnitude of 300 m, confront lots of technical challenges in design and construction. Several crucial technologies of 6 SHADs will be reviewed and discussed in this and consecutive papers, including Xiluodu, Jinping I in China, Baktiary in Iran, ete. , on the topics of the research method, criterion for evaluation and engineering application of dam safety analysis and evaluation, reasonable dam base interface, dam shape optimization, comprehensive treatments of complex foundation, anti-seismic engineering, dam construction material, concrete placement and temperature control, instrumentation and monitoring of dam operation, etc. This paper will mainly focus on the overall safety of SHADs, reasonable dam base interface analysis and evaluation and their engineering application.

  7. A new ab initio approach to the development of high temperature super conducting materials

    CERN Document Server

    Turner, Philip

    2016-01-01

    We review recent theoretical developments, which suggest that a set of shared principles underpin macroscopic quantum phenomena observed in high temperature super conducting materials, room temperature coherence in photosynthetic processes and the emergence of long range order in biological structures. These systems are driven by dissipative systems, which lead to fractal assembly and a fractal network of charges (with associated quantum potentials) at the molecular scale. At critical levels of charge density and fractal dimension, individual quantum potentials merge to form a charged induced macroscopic quantum potential, which act as a structuring force dictating long range order. Whilst the system is only partially coherent (i.e. only the bosonic fields are coherent), within these processes many of the phenomena associated with standard quantum theory are recovered, with macroscopic quantum potentials and associated forces having their equivalence in standard quantum mechanics. We establish a testable hypo...

  8. Development of High Level Trigger Software for Belle II at SuperKEKB

    Science.gov (United States)

    Lee, S.; Itoh, R.; Katayama, N.; Mineo, S.

    2011-12-01

    The Belle collaboration has been trying for 10 years to reveal the mystery of the current matter-dominated universe. However, much more statistics is required to search for New Physics through quantum loops in decays of B mesons. In order to increase the experimental sensitivity, the next generation B-factory, SuperKEKB, is planned. The design luminosity of SuperKEKB is 8 x 1035cm-2s-1 a factor 40 above KEKB's peak luminosity. At this high luminosity, the level 1 trigger of the Belle II experiment will stream events of 300 kB size at a 30 kHz rate. To reduce the data flow to a manageable level, a high-level trigger (HLT) is needed, which will be implemented using the full offline reconstruction on a large scale PC farm. There, physics level event selection is performed, reducing the event rate by ~ 10 to a few kHz. To execute the reconstruction the HLT uses the offline event processing framework basf2, which has parallel processing capabilities used for multi-core processing and PC clusters. The event data handling in the HLT is totally object oriented utilizing ROOT I/O with a new method of object passing over the UNIX socket connection. Also under consideration is the use of the HLT output as well to reduce the pixel detector event size by only saving hits associated with a track, resulting in an additional data reduction of ~ 100 for the pixel detector. In this contribution, the design and implementation of the Belle II HLT are presented together with a report of preliminary testing results.

  9. Barcoding bias in high-throughput multiplex sequencing of miRNA.

    Science.gov (United States)

    Alon, Shahar; Vigneault, Francois; Eminaga, Seda; Christodoulou, Danos C; Seidman, Jonathan G; Church, George M; Eisenberg, Eli

    2011-09-01

    Second-generation sequencing is gradually becoming the method of choice for miRNA detection and expression profiling. Given the relatively small number of miRNAs and improvements in DNA sequencing technology, studying miRNA expression profiles of multiple samples in a single flow cell lane becomes feasible. Multiplexing strategies require marking each miRNA library with a DNA barcode. Here we report that barcodes introduced through adapter ligation confer significant bias on miRNA expression profiles. This bias is much higher than the expected Poisson noise and masks significant expression differences between miRNA libraries. This bias can be eliminated by adding barcodes during PCR amplification of libraries. The accuracy of miRNA expression measurement in multiplexed experiments becomes a function of sample number.

  10. High-speed phonon imaging using frequency-multiplexed kinetic inductance detectors

    CERN Document Server

    Swenson, L J; Benoit, A; Roesch, M; Yung, C ~S; Bideaud, A; Monfardini, A

    2010-01-01

    We present a measurement of phonon propagation in a silicon wafer utilizing an array of frequency-multiplexed superconducting resonators coupled to a single transmission line. The electronic readout permits fully synchronous array sampling with a per-resonator bandwidth of 1.2 MHz, allowing sub-$\\mu$s array imaging. This technological achievement is potentially vital in a variety of low-temperature applications, including single-photon counting, quantum-computing and dark-matter searches.

  11. Parallel segmented outlet flow high performance liquid chromatography with multiplexed detection

    Energy Technology Data Exchange (ETDEWEB)

    Camenzuli, Michelle [Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney (Parramatta), Sydney, NSW (Australia); Terry, Jessica M. [Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216 (Australia); Shalliker, R. Andrew, E-mail: r.shalliker@uws.edu.au [Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney (Parramatta), Sydney, NSW (Australia); Conlan, Xavier A.; Barnett, Neil W. [Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216 (Australia); Francis, Paul S., E-mail: paul.francis@deakin.edu.au [Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3216 (Australia)

    2013-11-25

    Graphical abstract: -- Highlights: •Multiplexed detection for liquid chromatography. •‘Parallel segmented outlet flow’ distributes inner and outer portions of the analyte zone. •Three detectors were used simultaneously for the determination of opiate alkaloids. -- Abstract: We describe a new approach to multiplex detection for HPLC, exploiting parallel segmented outlet flow – a new column technology that provides pressure-regulated control of eluate flow through multiple outlet channels, which minimises the additional dead volume associated with conventional post-column flow splitting. Using three detectors: one UV-absorbance and two chemiluminescence systems (tris(2,2′-bipyridine)ruthenium(III) and permanganate), we examine the relative responses for six opium poppy (Papaver somniferum) alkaloids under conventional and multiplexed conditions, where approximately 30% of the eluate was distributed to each detector and the remaining solution directed to a collection vessel. The parallel segmented outlet flow mode of operation offers advantages in terms of solvent consumption, waste generation, total analysis time and solute band volume when applying multiple detectors to HPLC, but the manner in which each detection system is influenced by changes in solute concentration and solution flow rates must be carefully considered.

  12. High-performance Multiplexer-based Logic Synthesis Using Pass-transistor Logic

    Directory of Open Access Journals (Sweden)

    Shen-Fu Hsiao

    2002-01-01

    Full Text Available An automatic logic/circuit synthesizer is developed which takes several Boolean functions as input and generates netlist output with basic composing cells from the pass-transistor cell library containing only two types of cells: 2-to-1 multiplexers and inverters. The synthesis procedure first constructs efficient binary decision diagrams (BDDs for these Boolean functions considering both multi-function sharing and minimum width. Each node in the BDD trees is realized by using a 2-to-1 multiplexer (MUX of proper driving capability designed pass-transistor logic. The inverters are then inserted all along the MUX paths in order to improve the speed performance and to alleviate the voltage-drop problem. Several methods are proposed to reduce the critical path delay in the multiplexer-chains for generation of faster circuits. Compared to the recently proposed pass-transistor-based top-down design, our synthesizer has better speed and area performance due to the reduced number of cascaded inverters.

  13. High-sensitivity high-throughput chip based biosensor array for multiplexed detection of heavy metals

    Science.gov (United States)

    Yan, Hai; Tang, Naimei; Jairo, Grace A.; Chakravarty, Swapnajit; Blake, Diane A.; Chen, Ray T.

    2016-03-01

    Heavy metal ions released into the environment from industrial processes lead to various health hazards. We propose an on-chip label-free detection approach that allows high-sensitivity and high-throughput detection of heavy metals. The sensing device consists of 2-dimensional photonic crystal microcavities that are combined by multimode interferometer to form a sensor array. We experimentally demonstrate the detection of cadmium-chelate conjugate with concentration as low as 5 parts-per-billion (ppb).

  14. Super high precision 200 ppi liquid crystal display series; Chokoseido 200 ppi ekisho display series

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    In mobile equipment, in demand is a high precision liquid crystal display (LCD) having the power of expression equivalent to printed materials like magazines because of the necessity of displaying a large amount of information on a easily potable small screen. In addition, with the spread and high-quality image of digital still cameras, it is strongly desired to display photographed digital image data in high quality. Toshiba Corp., by low temperature polysilicone (p-Si) technology, commercialized the liquid crystal display series of 200 ppi (pixels per inch) precision dealing with the rise of the high-precision high-image quality LCD market. The super high precision of 200 ppi enables the display of smooth beautiful animation comparable to printed sheets of magazines and photographs. The display series are suitable for the display of various information services such as electronic books and electronic photo-viewers including internet. The screen sizes lined up are No. 4 type VGA (640x480 pixels) of a small pocket notebook size and No. 6.3 type XGA (1,024x768 pixels) of a paperback size, with a larger screen to be furthered. (translated by NEDO)

  15. High-temperature condensate clouds in super-hot Jupiter atmospheres

    Science.gov (United States)

    Wakeford, H. R.; Visscher, C.; Lewis, N. K.; Kataria, T.; Marley, M. S.; Fortney, J. J.; Mandell, A. M.

    2017-02-01

    Deciphering the role of clouds is central to our understanding of exoplanet atmospheres, as they have a direct impact on the temperature and pressure structure, and observational properties of the planet. Super-hot Jupiters occupy a temperature regime similar to low-mass M-dwarfs, where minimal cloud condensation is expected. However, observations of exoplanets such as WASP-12b (Teq ˜ 2500 K) result in a transmission spectrum indicative of a cloudy atmosphere. We re-examine the temperature and pressure space occupied by these super-hot Jupiter atmospheres, to explore the role of the initial Al- and Ti-bearing condensates as the main source of cloud material. Due to the high temperatures, a majority of the more common refractory material is not depleted into deeper layers and would remain in the vapour phase. The lack of depletion into deeper layers means that these materials with relatively low cloud masses can become significant absorbers in the upper atmosphere. We provide condensation curves for the initial Al- and Ti-bearing condensates which may be used to provide quantitative estimates of the effect of metallicity on cloud masses, as planets with metal-rich hosts potentially form more opaque clouds because more mass is available for condensation. Increased metallicity also pushes the point of condensation to hotter, deeper layers in the planetary atmosphere further increasing the density of the cloud. We suggest that planets around metal-rich hosts are more likely to have thick refractory clouds, and discuss the implication on the observed spectra of WASP-12b.

  16. Detection and typing of highly pathogenic porcine reproductive and respiratory syndrome virus by multiplex real-time rt-PCR.

    Directory of Open Access Journals (Sweden)

    Kerstin Wernike

    Full Text Available Porcine reproductive and respiratory syndrome (PRRS causes economic losses in the pig industry worldwide, and PRRS viruses (PRRSV are classified into the two distinct genotypes "North American (NA, type 2" and "European (EU, type 1". In 2006, a highly pathogenic NA strain of PRRSV (HP-PRRSV, characterized by high fever as well as high morbidity and mortality, emerged in swine farms in China. Therefore, a real-time reverse transcription polymerase chain reaction (RT-qPCR assay specific for HP-PRRSV was developed and combined with type 1- and type 2-specific RT-qPCR systems. Furthermore, an internal control, based on a heterologous RNA, was successfully introduced. This final multiplex PRRSV RT-qPCR, detecting and typing PRRSV, had an analytical sensitivity of less than 200 copies per µl for the type 1-assay and 20 copies per µl for the type 2- and HP assays and a high diagnostic sensitivity. A panel of reference strains and field isolates was reliably detected and samples from an animal trial with a Chinese HP-PRRS strain were used for test validation. The new multiplex PRRSV RT-qPCR system allows for the first time the highly sensitive detection and rapid differentiation of PRRSV of both genotypes as well as the direct detection of HP-PRRSV.

  17. Stabilized super-thermite colloids: A new generation of advanced highly energetic materials

    Science.gov (United States)

    Elbasuney, Sherif; Gaber Zaky, M.; Radwan, Mostafa; Mostafa, Sherif F.

    2017-10-01

    One of the great impetus of nanotechnology on energetic materials is the achievement of nanothermites (metal-oxide/metal) which are characterized by massive heat output. Yet, full exploitation of super-thermites in highly energetic systems has not been achieved. This manuscript reports on the sustainable fabrication of colloidal Fe2O3 and CuO nanoparticles for thermite applications. TEM micrographs demonstrated mono-dispersed Fe2O3 and CuO with an average particle size of 3 and 15 nm respectively. XRD diffractograms demonstrated highly crystalline materials. SEM micrographs demonstrated a great tendency of the developed oxides to aggregate over drying process. The effective integration and dispersion of mono-dispersed colloidal thermite particles into energetic systems are vital for enhanced performance. Aluminum is of interest as highly energetic metal fuel. In this paper, synthesized Fe2O3 and CuO nanoparticles were re-dispersed in isopropyl alcohol (IPA) with aluminum nanoparticles using ultrasonic prope homogenizer. The colloidal thermite peraticles can be intgegrated into highly energetic system for subsequent nanocomposite development. Thanks to stabilization of colloidal CuO nanoparticles in IPA which could offer intimate mixing between oxidizer and metal fuel. The stabilization mechanism of CuO in IPA was correlated to steric stabilization with solvent molecules. This approach eliminated nanoparticle drying and the re-dispersion of dry aggregates into energetic materials. This manuscript shaded the light on the real development of colloidal thermite mixtures and their integration into highly energetic systems.

  18. Over-the-air demonstration of spatial multiplexing at high data rates using real-time base-band processing

    Directory of Open Access Journals (Sweden)

    V. Jungnickel

    2004-01-01

    Full Text Available Over-the-air transmission experiments with a realtime MIMO test-bed are reported. We describe in principle a hardware architecture for spatial multiplexing at high data rates, discuss in detail the implementation on a hybrid FPGA/DSP platform and show measured bit error rates from indoor transmission experiments. Per-antenna rate control and joint transmission are enabled as well using an ideal feed-back link. A functional test of these new techniques is described while detailed transmission experiments are still ongoing.

  19. Productive and vegetative behavior of olive cultivars in super high-density olive grove

    Directory of Open Access Journals (Sweden)

    Primo Proietti

    2015-02-01

    Full Text Available In recent years, there has been an increase in interest in super high-density (SHD olive (Olea europaea L. groves because they offer early entry into production, increased productivity and the possibility of using modified mechanical vine harvesters. This study was carried out in a young SHD olive grove to examine vegetative, histo-anatomical and productive characteristics and oil quality of the Spanish Arbequina and Italian Maurino and Leccino cultivars, characterized by low, low-to-medium and high vigor, respectively. Arbequina had low vigor and limited development in height and width, as well as a high leaf/wood ratio. Maurino had a canopy volume similar to that of Arbequina and, despite a great tendency to grow in height, had low vigor, a rather compact vegetative habitus, but good lighting in the canopy and high production efficiency. In Maurino, a greater palisade parenchyma height and a larger exposed lateral surface area of the palisade parenchyma cells were observed. In the fourth year after planting, fruit production of Arbequina was about 30 % less than Leccino and Maurino. The oil content on a dry weight basis was slightly higher in Arbequina and Maurino than in Leccino. Oil quality was good for all cultivars.

  20. Multiplexity and multireciprocity in directed multiplexes

    Science.gov (United States)

    Gemmetto, Valerio; Squartini, Tiziano; Picciolo, Francesco; Ruzzenenti, Franco; Garlaschelli, Diego

    2016-10-01

    Real-world multilayer networks feature nontrivial dependencies among links of different layers. Here we argue that if links are directed, then dependencies are twofold. Besides the ordinary tendency of links of different layers to align as the result of "multiplexity," there is also a tendency to antialign as a result of what we call "multireciprocity," i.e., the fact that links in one layer can be reciprocated by opposite links in a different layer. Multireciprocity generalizes the scalar definition of single-layer reciprocity to that of a square matrix involving all pairs of layers. We introduce multiplexity and multireciprocity matrices for both binary and weighted multiplexes and validate their statistical significance against maximum-entropy null models that filter out the effects of node heterogeneity. We then perform a detailed empirical analysis of the world trade multiplex (WTM), representing the import-export relationships between world countries in different commodities. We show that the WTM exhibits strong multiplexity and multireciprocity, an effect which is, however, largely encoded into the degree or strength sequences of individual layers. The residual effects are still significant and allow us to classify pairs of commodities according to their tendency to be traded together in the same direction and/or in opposite ones. We also find that the multireciprocity of the WTM is significantly lower than the usual reciprocity measured on the aggregate network. Moreover, layers with low (high) internal reciprocity are embedded within sets of layers with comparably low (high) mutual multireciprocity. This suggests that, in the WTM, reciprocity is inherent to groups of related commodities rather than to individual commodities. We discuss the implications for international trade research focusing on product taxonomies, the product space, and fitness and complexity metrics.

  1. High-performance supercapacitors using a nanoporous current collector made from super-aligned carbon nanotubes

    Energy Technology Data Exchange (ETDEWEB)

    Zhou Ruifeng; Meng Chuizhou; Zhu Feng; Li Qunqing; Liu Changhong; Fan Shoushan; Jiang Kaili, E-mail: JiangKL@tsinghua.edu.cn [Department of Physics and Tsinghua-Foxconn Nanotechnology Research Center, Tsinghua University, Beijing 100084 (China)

    2010-08-27

    Nanoporous current collectors for supercapacitors have been fabricated by cross-stacking super-aligned carbon nanotube (SACNT) films as a replacement for heavy conventional metallic current collectors. The CNT-film current collectors have good conductivity, extremely low density (27 {mu}g cm{sup -2}), high specific surface area, excellent flexibility and good electrochemical stability. Nanosized active materials such as NiO, Co{sub 3}O{sub 4} or Mn{sub 2}O{sub 3} nanoparticles can be directly synthesized on the SACNT films by a straightforward one-step, in situ decomposition strategy that is both efficient and environmentally friendly. These composite films can be integrated into a pseudo-capacitor that does not use metallic current collectors, but nevertheless shows very good performance, including high specific capacitance ({approx}500 F g{sup -1}, including the current collector mass), reliable electrochemical stability (<4.5% degradation in 2500 cycles) and a very high rate capability (245 F g{sup -1} at 155 A g{sup -1}).

  2. High-Capacity Angularly Multiplexed Holographic Memory Operating at the Single-Photon Level.

    Science.gov (United States)

    Chrapkiewicz, Radosław; Dąbrowski, Michał; Wasilewski, Wojciech

    2017-02-10

    We experimentally demonstrate an angularly multiplexed holographic memory capable of intrinsic generation, storage, and retrieval of multiple photons, based on an off-resonant Raman interaction in warm rubidium-87 vapors. The memory capacity of up to 60 independent atomic spin-wave modes is evidenced by analyzing angular distributions of coincidences between Stokes and time-delayed anti-Stokes light, observed down to the level of single spin-wave excitation during the several-microsecond memory lifetime. We also propose how to practically enhance rates of single- and multiple-photon generation by combining our multimode emissive memory with existing fast optical switches.

  3. High-latitude poynting flux from combined Iridium and SuperDARN data

    Directory of Open Access Journals (Sweden)

    C. L. Waters

    2004-09-01

    Full Text Available Field-aligned currents convey stress between the magnetosphere and ionosphere, and the associated low altitude magnetic and electric fields reflect the flow of electromagnetic energy to the polar ionosphere. We introduce a new technique to measure the global distribution of high latitude Poynting flux, S||, by combining electric field estimates from the Super Dual Auroral Radar Network (SuperDARN with magnetic perturbations derived using magnetometer data from the Iridium satellite constellation. Spherical harmonic methods are used to merge the data sets and calculate S|| for any magnetic local time (MLT from the pole to 60° magnetic latitude (MLAT. The effective spatial resolutions are 2° MLAT, 2h MLT, and the time resolution is about one hour due to the telemetry rate of the Iridium magnetometer data. The technique allows for the assessment of high-latitude net S|| and its spatial distribution on one hour time scales with two key advantages: (1 it yields the net S|| including the contribution of neutral winds; and (2 the results are obtained without recourse to estimates of ionosphere conductivity. We present two examples, 23 November 1999, 14:00-15:00 UT, and 11 March 2000, 16:00-17:00 UT, to test the accuracy of the technique and to illustrate the distributions of S|| that it gives. Comparisons with in-situ S|| estimates from DMSP satellites show agreement to a few mW/m2 and in the locations of S|| enhancements to within the technique's resolution. The total electromagnetic energy flux was 50GW for these events. At auroral latitudes, S|| tends to maximize in the morning and afternoon in regions less than 5° in MLAT by two hours in MLT having S||=10 to 20mW/m2 and total power up to 10GW. The power poleward of the Region 1 currents is about one

  4. Optimization design of foundation excavation for Xiluodu super-high arch dam in China

    Institute of Scientific and Technical Information of China (English)

    Qixiang Fan; Shaowu Zhou; Ning Yang

    2015-01-01

    With better understanding of the quality and physico-mechanical properties of rocks of dam foundation, and the physico-mechanical properties and structure design of arch dam in association with the foun-dation excavation of Xiluodu arch dam, the excavation optimization design was proposed for the foundation surface on the basis of feasibility study. Common analysis and numerical analysis results demonstrated the feasibility of using the weakly weathered rocks III1 and III2 as the foundation surface of super-high arch dam. In view of changes in the geological conditions at the dam foundation along the riverbed direction, the design of extending foundation surface excavation area and using consolidating grouting and optimizing structure of dam bottom was introduced, allowing for harmonization of the arch dam and foundation. Three-dimensional (3D) geomechanics model test and finite element analysis re-sults indicated that the dam body and foundation have good overload stability and high bearing capacity. The monitoring data showed that the behaviors of dam and foundation correspond with the designed patterns in the construction period and the initial operation period.

  5. Optimization design of foundation excavation for Xiluodu super-high arch dam in China

    Directory of Open Access Journals (Sweden)

    Qixiang Fan

    2015-04-01

    Full Text Available With better understanding of the quality and physico-mechanical properties of rocks of dam foundation, and the physico-mechanical properties and structure design of arch dam in association with the foundation excavation of Xiluodu arch dam, the excavation optimization design was proposed for the foundation surface on the basis of feasibility study. Common analysis and numerical analysis results demonstrated the feasibility of using the weakly weathered rocks III1 and III2 as the foundation surface of super-high arch dam. In view of changes in the geological conditions at the dam foundation along the riverbed direction, the design of extending foundation surface excavation area and using consolidating grouting and optimizing structure of dam bottom was introduced, allowing for harmonization of the arch dam and foundation. Three-dimensional (3D geomechanics model test and finite element analysis results indicated that the dam body and foundation have good overload stability and high bearing capacity. The monitoring data showed that the behaviors of dam and foundation correspond with the designed patterns in the construction period and the initial operation period.

  6. High-power production targets for the Super-FRS using a fast extraction scheme

    CERN Document Server

    Tahir, N A; Kojouharova, J; Roussel-Chomaz, P; Chishkine, V; Geissel, H; Hoffmann, Dieter H H; Kindler, B; Landre-Pellemoine, F; Lommel, B; Mittig, W; Münzenberg, G; Shutov, A; Weick, H; Yavor, M

    2003-01-01

    The high-power production target of the Super-FRS [H. Geissel et al., these Proceedings] will be irradiated by very intense heavy-ion beams which will be delivered from the future SIS100/200 [An International Accelerator Facility for Beams of Ions and Antiprotons, GSI-Report, 2001] synchrotron facility at the Gesellschaft fuer Schwerionenforschung (GSI) Darmstadt. This paper presents calculations of the thermodynamic and the hydrodynamic response of such a target, considering that a uranium ion beam with an energy of 1 GeV/u and an intensity of 10 sup 1 sup 2 particles will impinge within 50 ns on a solid carbon target with a thickness of 4 g/cm sup 2. Due to the high beam intensity the target may be strongly heated and could be destroyed in a single shot. The purpose of this work is to investigate with the help of two-dimensional numerical simulations how one can minimize target heating and avoid target destruction.

  7. Ultra - High Energy Cosmic Rays from decay of the Super Heavy Dark Matter Relics

    CERN Document Server

    Doroshkevich, A G

    2000-01-01

    In this paper we briefly discuss the problem of the origin of Ultra High Energy Cosmic Rays in the framework of Top-Down models. We show that, for high energy of decays and in a wide range of spectra of injected protons, their extragalactic flux is consistent with the observed fluxes of cosmic rays in the energy range 0.1 E_{GZK}< E < 10E_{GZK}. For suitable energy and spectra of injected protons, the contribution of galactic sources is moderate, in this energy range, but it dominates at smaller and larger energies. In such models we can expect that at these energies the anisotropy of cosmic rays distribution over sky will be especially small. Some possible manifestations of decays of super massive particles such as, for example, primordial black holes with masses M_{pbh} ~ 10^{-5} g, are considered. In particular, we show that partial conversion of energy released during these decays at redshifts z ~ 1000 to Ly-alpha photons can delay the hydrogen recombination and distort the spectrum of fluctuations ...

  8. A New Approach for Refining Carbide Dimensions in M42 Super Hard High-speed Steel

    Institute of Scientific and Technical Information of China (English)

    Xue-feng ZHOU; Wang-long ZHU; Hong-bing JIANG; Feng FANG; Yi-you TU; Jian-qing JIANG

    2016-01-01

    Obtaining small carbides is crucial but difficult for high-speed steels.A new approach for refining carbide dimensions in M42 super hard high-speed steel by increasing cooling rate and spheroidizing treatment was proposed. The morphologies and properties of eutectic carbides formed at different cooling rates were investigated by means of scanning electron microscopy (SEM),energy dispersive spectroscopy (EDS),X-ray diffraction (XRD),transmis-sion electron microscopy (TEM),electron back-scattered diffraction (EBSD)and differential scanning calorimeter (DSC).The results show that eutectic carbides change from a lamellar shape into a curved-rod shape as cooling rate increases.Despite different morphologies,the two carbides are both of M2 C type with a hexagonal close-packed structure and display a single crystal orientation in one eutectic colony.The morphology of M2 C mainly depends on the growing process of eutectic carbides,which is strongly influenced by cooling rate.Compared with lamellar car-bides,M2 C carbides with curved-rod shapes are less stable,and decompose into M6 C and MC at lower temperatures. They are more inclined to spheroidize during heating,which ultimately and distinguishably refines the carbide dimen-sions.As small carbides are much easier to dissolve into matrices during austenization,the process described herein improves the supersaturation of alloying elements in martensite,which leads to an increment of hardness in M42 steel.

  9. High-capacity millimetre-wave communications with orbital angular momentum multiplexing.

    Science.gov (United States)

    Yan, Yan; Xie, Guodong; Lavery, Martin P J; Huang, Hao; Ahmed, Nisar; Bao, Changjing; Ren, Yongxiong; Cao, Yinwen; Li, Long; Zhao, Zhe; Molisch, Andreas F; Tur, Moshe; Padgett, Miles J; Willner, Alan E

    2014-09-16

    One property of electromagnetic waves that has been recently explored is the ability to multiplex multiple beams, such that each beam has a unique helical phase front. The amount of phase front 'twisting' indicates the orbital angular momentum state number, and beams with different orbital angular momentum are orthogonal. Such orbital angular momentum based multiplexing can potentially increase the system capacity and spectral efficiency of millimetre-wave wireless communication links with a single aperture pair by transmitting multiple coaxial data streams. Here we demonstrate a 32-Gbit s(-1) millimetre-wave link over 2.5 metres with a spectral efficiency of ~16 bit s(-1) Hz(-1) using four independent orbital-angular momentum beams on each of two polarizations. All eight orbital angular momentum channels are recovered with bit-error rates below 3.8 × 10(-3). In addition, we demonstrate a millimetre-wave orbital angular momentum mode demultiplexer to demultiplex four orbital angular momentum channels with crosstalk less than -12.5 dB and show an 8-Gbit s(-1) link containing two orbital angular momentum beams on each of two polarizations.

  10. High-throughput mass-directed parallel purification incorporating a multiplexed single quadrupole mass spectrometer.

    Science.gov (United States)

    Xu, Rongda; Wang, Tao; Isbell, John; Cai, Zhe; Sykes, Christopher; Brailsford, Andrew; Kassel, Daniel B

    2002-07-01

    We report on the development of a parallel HPLC/MS purification system incorporating an indexed (i.e., multiplexed) ion source. In the method described, each of the flow streams from a parallel array of HPLC columns is directed toward the multiplexed (MUX) ion source and sampled in a time-dependent, parallel manner. A visual basic application has been developed and monitors in real-time the extracted ion current from each sprayer channel. Mass-directed fraction collection is initiated into a parallel array of fraction collectors specific for each of the spray channels. In the first embodiment of this technique, we report on a four-column semipreparative parallel LC/MS system incorporating MUX detection. In this parallel LC/MS application (in which sample loads between 1 and 10 mg on-column are typically made), no cross talk was observed. Ion signals from each of the channels were found reproducible over 192 injections, with interchannel signal variations between 11 and 17%. The visual basic fraction collection application permits preset individual start collection and end collection thresholds for each channel, thereby compensating for the slight variation in signal between sprayers. By incorporating postfraction collector UV detection, we have been able to optimize the valve-triggering delay time with precut transfer tubing between the mass spectrometer and fraction collectors and achieve recoveries greater than 80%. Examples of the MUX-guided, mass-directed fraction purification of both standards and real library reaction mixtures are presented within.

  11. Preparation and application of hollow molecularly imprinted polymers with a super-high selectivity to the template protein.

    Science.gov (United States)

    Chen, Yang; He, Xi-Wen; Mao, Jie; Li, Wen-You; Zhang, Yu-Kui

    2013-10-01

    Protein-imprinted polymers with hollow cores that have a super-high imprinting factor were prepared by etching the core of the surface-imprinted polymers that used silica particles as the support. Lysozyme as template was modified onto the surface of silica particles by a covalent method, and after polymerization and the removal of template molecules, channels through the polymer layer were formed, which allowed a single-protein molecule to come into the hollow core and attach to the binding sites inside the polymer layer. The adsorption experiments demonstrated that the hollow imprinted polymers had an extremely high binding capacity and selectivity, and thus a super-high imprinting factor was obtained. The as-prepared imprinted polymers were used to separate the template lysozyme from egg white successfully, indicating its high selectivity and potential application in the field of separation of protein from real samples.

  12. Segmented continuous-flow multiplex polymerase chain reaction microfluidics for high-throughput and rapid foodborne pathogen detection.

    Science.gov (United States)

    Shu, Bowen; Zhang, Chunsun; Xing, Da

    2014-05-15

    High-throughput and rapid identification of multiple foodborne bacterial pathogens is vital in global public health and food industry. To fulfill this need, we propose a segmented continuous-flow multiplex polymerase chain reaction (SCF-MPCR) on a spiral-channel microfluidic device. The device consists of a disposable polytetrafluoroethylene (PTFE) capillary microchannel coiled on three isothermal blocks. Within the channel, n segmented flow regimes are sequentially generated, and m-plex PCR is individually performed in each regime when each mixture is driven to pass three temperature zones, thus providing a rapid analysis throughput of m×n. To characterize the performance of the microfluidic device, continuous-flow multiplex PCR in a single segmented flow has been evaluated by investigating the effect of key reaction parameters, including annealing temperatures, flow rates, polymerase concentration and amount of input DNA. With the optimized parameters, the genomic DNAs from Salmonella enterica, Listeria monocytogenes, Escherichia coli O157:H7 and Staphylococcus aureus could be amplified simultaneously in 19min, and the limit of detection was low, down to 10(2) copiesμL(-1). As proof of principle, the spiral-channel SCF-MPCR was applied to sequentially amplify four different bacterial pathogens from banana, milk, and sausage, displaying a throughput of 4×3 with no detectable cross-contamination.

  13. Dynamic analyses of steel-reinforced super high-strength concrete columns

    Institute of Scientific and Technical Information of China (English)

    JIA Jinqing; JIANG Rui; HOU Tong; XU Shilang

    2007-01-01

    The dynamic behaviors of steel-reinforced super high-strength concrete columns under seismic loading were studied with a series of experiments. Thirteen specimens, with concrete strengths ranging from 94.9 to 105.4 Mpa and shear-span ratios of 2.75, were manufactured. The axial load ratio and the stirrup reinforcement ratio were the main experimental variables affecting the dynamic behavior of the specimens. The columns under low cyclic lateral loading mainly failed in the flexural-shear mode. Shear force- displacement hysteretic curves and skeleton curves were drawn. The coefficients of the specimens' displacement ductility were calculated. Experimental results indicate that ductility decreases while the axial load ratio increases, but it increases when the stirrup reinforcement ratio increases. The limit values of the axial load ratio and the minimum stirrup reinforcement ratio of the columns were investigated to satisfy definite ductility requirements. These values were suggested as references of engineering applications and of the amendment of the current Chinese design code of steel reinforced concrete composite structures.

  14. Application of evaporative cooling technology in super-high power density magnet.

    Science.gov (United States)

    Xiong, B; Ruan, L; Gu, G B; Guo, S Q; Cao, R; Li, Z G; Lu, W; Zhang, X Z; Sun, L T; Zhao, H W

    2014-02-01

    Evaporative cooling technology utilizes phase-change heat transfer mode to achieve the cooling for heating equipment. The heat transfer capacity of evaporative cooling technology is far more than air or water cooling technology. The Electron Cyclotron Resonance ion source magnet is a typical super-high power density magnet, and the evaporative cooling technology is an ideal cooling method for the coils of magnet. In this paper we show the structure and process of coils and the special design of flow channels of coolant for an experiment magnet model. Additionally, the heat transfer circulation is presented and analyzed. By the finite element method, the flow channels are optimized to rationally allocate coolant and to reduce the temperature of coils. For the experiment model, the current density of copper wire of coils is 19 A/mm(2), and the coil-windows current density is larger than 12 A/mm(2). The max temperature of coils is below 80 °C, and the total heat is about 200 kW.

  15. Kinetics and Microstructural Investigation of High-Temperature Oxidation of IN-738LC Super Alloy

    Science.gov (United States)

    Hamidi, S.; Rahimipour, M. R.; Eshraghi, M. J.; Hadavi, S. M. M.; Esfahani, H.

    2017-02-01

    The present study was carried out to investigate the kinetics and the surface chemistry of the oxide layers formed on the IN-738LC super alloy during high-temperature oxidation at 950 °C in air from 1 to 260 h. Oxidation kinetics were studied by mass gain measurement. The oxide layers were characterized by field emission scanning electron microscope, elemental distribution map, energy-dispersive spectroscopy as well as x-ray diffractometry (XRD). The oxidation kinetics followed the parabolic law. The XRD analysis revealed that the oxide scale contained mainly NiO, Ni (Cr, Al)2O4, Al2O3, TiO2 and Cr2O3. The oxide structure, from the top surface down to the substrate, was clarified by elemental map distribution studies as Ni-Ti oxides, Cr-Ti oxides, Cr2O3 oxide band, Ni-Co-Cr-W oxide and finally a blocky Al2O3 region. The oxidation scales were composed of three distinct layers of the outer and mid layers enriched by TiO2 and Cr2O3, NiCr2O4 oxide, respectively, and the innermost layer was composed of Al2O3 and matrix alloy. The depleted gamma prime layer was formed under the oxidation scales due to the impoverishment of Al and Ti which were induced by the formation of Al2O3 and TiO2.

  16. Simultaneous frequency stabilization and high-power dense wavelength division multiplexing (HP-DWDM) using an external cavity based on volume Bragg gratings (VBGs)

    Science.gov (United States)

    Hengesbach, Stefan; Klein, Sarah; Holly, Carlo; Witte, Ulrich; Traub, Martin; Hoffmann, Dieter

    2016-03-01

    Multiplexing technologies enable the development of high-brightness diode lasers for direct industrial applications. We present a High-Power Dense Wavelength Division Multiplexer (HP-DWDM) with an average channel spacing of 1.7 (1.5) nm and a subsequent external cavity mirror to provide feedback for frequency stabilization and multiplexing in one step. The "self-optimizing" multiplexing unit consists of four reflective Volume Bragg Gratings (VBGs) with 99% diffraction efficiency and seven dielectric mirrors to overlay the radiation of five input channels with an adjustable channel spacing of 1-2 nm. In detail, we focus on the analysis of the overall optical efficiency, the change of the beam parameter product and the spectral width. The performance is demonstrated using five 90 μm multimode 9xx single emitters with M2angular intensity distribution changes strongly and the beam parameter product decreases by a factor of 1.2 to 1.9. Thereby the angular intensity distribution is more affected than the width of the beam waist. The spectral width per emitter decreases to 3-200 pm (FWHM) depending on the injection current and the reflectance of the feedback mirror (0.75%, 1.5%, 4%, 6% or 8%). The overall optical multiplexing efficiency ranges between 77% and 86%. With some modifications (e.g. enhanced AR-coatings) we expect 90-95%.

  17. Advancing the High Throughput Identification of Liver Fibrosis Protein Signatures Using Multiplexed Ion Mobility Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Baker, Erin Shammel; Burnum-Johnson, Kristin E.; Jacobs, Jon M.; Diamond, Deborah L.; Brown, Roslyn N.; Ibrahim, Yehia M.; Orton, Daniel J.; Piehowski, Paul D.; Purdy, David E.; Moore, Ronald J.; Danielson, William F.; Monroe, Matthew E.; Crowell, Kevin L.; Slysz, Gordon W.; Gritsenko, Marina A.; Sandoval, John D.; Lamarche, Brian L.; Matzke, Melissa M.; Webb-Robertson, Bobbie-Jo M.; Simons, Brenna C.; McMahon, Brian J.; Bhattacharya, Renuka; Perkins, James D.; Carithers, Robert L.; Strom, Susan; Self, Steven; Katze, Michael G.; Anderson, Gordon A.; Smith, Richard D.

    2014-04-01

    Rapid diagnosis of disease states using less invasive, safer, and more clinically acceptable approaches than presently employed is an imperative goal for the field of medicine. While mass spectrometry (MS)-based proteomics approaches have attempted to meet these objectives, challenges such as the enormous dynamic range of protein concentrations in clinically relevant biofluid samples coupled with the need to address human biodiversity have slowed their employment. Herein, we report on the use of a new platform that addresses these challenges by coupling technical advances in rapid gas phase multiplexed ion mobility spectrometry (IMS) separations [1, 2] with liquid chromatography (LC) and MS to dramatically increase measurement sensitivity and throughput, further enabling future MS-based clinical applications. An initial application of the LC-IMS-MS platform for the analysis of blood serum samples from stratified post-liver transplant patients with recurrent fibrosis progression illustrates its potential utility for disease characterization and use in personalized medicine [3, 4].

  18. High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system.

    Science.gov (United States)

    Cobb, Ryan E; Wang, Yajie; Zhao, Huimin

    2015-06-19

    Actinobacteria, particularly those of genus Streptomyces, remain invaluable hosts for the discovery and engineering of natural products and their cognate biosynthetic pathways. However, genetic manipulation of these bacteria is often labor and time intensive. Here, we present an engineered CRISPR/Cas system for rapid multiplex genome editing of Streptomyces strains, demonstrating targeted chromosomal deletions in three different Streptomyces species and of various sizes (ranging from 20 bp to 30 kb) with efficiency ranging from 70 to 100%. The designed pCRISPomyces plasmids are amenable to assembly of spacers and editing templates via Golden Gate assembly and isothermal assembly (or traditional digestion/ligation), respectively, allowing rapid plasmid construction to target any genomic locus of interest. As such, the pCRISPomyces system represents a powerful new tool for genome editing in Streptomyces.

  19. Multiplex polymerase chain reaction for the detection of high-risk-human papillomavirus types in formalin-fixed paraffin-embedded cervical tissues

    Directory of Open Access Journals (Sweden)

    Mini P Singh

    2017-01-01

    Full Text Available Detecting high-risk-human papillomavirus (HPV types has become an integral part of the cervical cancer screening programmes. This study aimed to develop a multiplex polymerase chain reaction (PCR for identification of HPV types 16 and 18 along with the beta globin gene in formalin-fixed and paraffin-embedded cervical biopsy specimens. A total of 59 samples from patients with cervical abnormalities were tested. HPV 16 positivity was 50% in cervical cancers and 52.9% in cervical intraepithelial neoplasia. Our multiplex PCR protocol can be used as a simple and cost-effective tool for high-risk-HPV detection in cervical cancer screening programmes.

  20. Viewing-distance aware super-resolution for high-definition display.

    Science.gov (United States)

    Shen, Chih-Tsung; Liu, Hung-Hsun; Yang, Ming-Hsuan; Hung, Yi-Ping; Pei, Soo-Chang

    2015-01-01

    In this paper, we propose a novel algorithm for high-definition displays to enlarge low-resolution images while maintaining perceptual constancy (i.e., the same field-of-view, perceptual blur radius, and the retinal image size in viewer's eyes). We model the relationship between a viewer and a display by considering two main aspects of visual perception, i.e., scaling factor and perceptual blur radius. As long as we enlarge an image while adjust its image blur levels on the display, we can maintain viewer's perceptual constancy. We show that the scaling factor should be set in proportion to the viewing distance and the blur levels on the display should be adjusted according to the focal length of a viewer. Toward this, we first refer to edge directions to interpolate a low-resolution image with the increasing of viewing distance and the scaling factor. After images are interpolated, we utilize a local contrast to estimate the spatially varying image blur levels of the interpolated image. We then further adjust the image blur levels using a parametric deblurring method, which combines L1 as well as L2 reconstruction errors, and Tikhonov with total variation regularization terms. By taking these factors into account, high-resolution images adaptive to viewing distance on a display can be generated. Experimental results on both natural image metric and user subjective studies across image scales demonstrate that the proposed super-resolution algorithm for high-definition displays performs favorably against the state-of-the-art methods.

  1. Exploiting the multiplexing capabilities of tandem mass tags for high-throughput estimation of cellular protein abundances by mass spectrometry.

    Science.gov (United States)

    Ahrné, Erik; Martinez-Segura, Amalia; Syed, Afzal Pasha; Vina-Vilaseca, Arnau; Gruber, Andreas J; Marguerat, Samuel; Schmidt, Alexander

    2015-09-01

    The generation of dynamic models of biological processes critically depends on the determination of precise cellular concentrations of biomolecules. Measurements of system-wide absolute protein levels are particularly valuable information in systems biology. Recently, mass spectrometry based proteomics approaches have been developed to estimate protein concentrations on a proteome-wide scale. However, for very complex proteomes, fractionation steps are required, increasing samples number and instrument analysis time. As a result, the number of full proteomes that can be routinely analyzed is limited. Here we combined absolute quantification strategies with the multiplexing capabilities of isobaric tandem mass tags to determine cellular protein abundances in a high throughput and proteome-wide scale even for highly complex biological systems, such as a whole human cell line. We generated two independent data sets to demonstrate the power of the approach regarding sample throughput, dynamic range, quantitative precision and accuracy as well as proteome coverage in comparison to existing mass spectrometry based strategies.

  2. Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize

    Institute of Scientific and Technical Information of China (English)

    WANG FengGe; ZHAO JiuRan; DAI JingRui; YI HongMei; KUANG Meng; SUN YanMei; YU XinYan; GUO JingLun; WANG Lu

    2007-01-01

    In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome.

  3. Super-Twisting Differentiator-Based High Order Sliding Mode Voltage Control Design for DC-DC Buck Converters

    Directory of Open Access Journals (Sweden)

    Yigeng Huangfu

    2016-06-01

    Full Text Available This paper aims to focus on the smooth output of DC-DC buck converters in wireless power transfer systems under input perturbations and load disturbances using the high-order sliding mode controller (HOSM and HOSM with super-twisting differentiator (HOSM + STD. The proposed control approach needs only measurement of converter output voltage. Theoretical analysis and design procedures, as well as the super-twisting differentiator of the proposed controller are presented in detail with the prescribed convergence law of high-order sliding modes. Comparisons of both simulation and experimental results among conventional proportional-integral (PI control, traditional sliding mode control (SMC, HOSM and HOSM + STD under various test conditions such as steady state, input voltage perturbations and output load disturbances, are presented and discussed. The results demonstrate and validate the effectiveness and robustness of the proposed control method.

  4. High blocking temperature in SnO{sub 2} based super-paramagnetic diluted magnetic semiconductor

    Energy Technology Data Exchange (ETDEWEB)

    Mounkachi, O., E-mail: o.mounkachi@mascir.com [Institute of Nanomaterials and Nanotechnology, MAScIR, Rabat (Morocco); Institut Néel, CNRS et Université Joseph Fourier, BP 166, F-38042 Grenoble cedex 9 (France); Salmani, E. [LMPHE, associé au CNRST (URAC 12), Faculté des Sciences, Université Mohammed V-Agdal, Rabat (Morocco); El Moussaoui, H. [Institute of Nanomaterials and Nanotechnology, MAScIR, Rabat (Morocco); Masrour, R. [Laboratory of Materials, Processes, Environment and Quality, Cady Ayyed University, National School of Applied Sciences, Safi (Morocco); Institut Néel, CNRS et Université Joseph Fourier, BP 166, F-38042 Grenoble cedex 9 (France); Hamedoun, M. [Institute of Nanomaterials and Nanotechnology, MAScIR, Rabat (Morocco); Ez-Zahraouy, H. [LMPHE, associé au CNRST (URAC 12), Faculté des Sciences, Université Mohammed V-Agdal, Rabat (Morocco); Hlil, E.K. [Institut Néel, CNRS et Université Joseph Fourier, BP 166, F-38042 Grenoble cedex 9 (France); Benyoussef, A. [Institute of Nanomaterials and Nanotechnology, MAScIR, Rabat (Morocco); LMPHE, associé au CNRST (URAC 12), Faculté des Sciences, Université Mohammed V-Agdal, Rabat (Morocco)

    2014-11-25

    Highlights: • Simple doping, (Sn,Fe)O{sub 2} exhibits a soft ferromagnetism at low temperature. • High blocking temperature was observed for Cu doped (Sn,Fe)O{sub 2} nanocrystalline. • Experimental results are confirmed by ab initio calculations. - Abstract: (Fe,Cu)-doped SnO{sub 2} nanocrystals was synthesized using the co-precipitation method. Magnetic Properties Measurement System (MPMS) revealed that for simple doping, Fe-doped SnO{sub 2} soft ferromagnetism at low temperature appears, while the ferromagnetic phase is stable at temperature higher than room temperature for Cu co-doping element. The ferromagnetism is significantly enhanced by the Cu addition to Fe-doped SnO{sub 2}, according to the ZFC and FC magnetizations and the hysteresis loops. The evidences for the existence of superparamagnetism are characterized and high blocking temperature super-paramagnetism in (Fe,Cu)-doped SnO{sub 2} nanocrystals was observed. Based on first-principles calculations, we have investigated electronic structures and magnetic properties of Fe-doped SnO{sub 2} and (Fe,Cu)-doped SnO{sub 2} with and without defect with LDA and LDA-SIC approximations. The results suggest that the oxygen vacancies (V{sub O}) play a critical role in the activation of ferromagnetism in Fe doped SnO{sub 2}. For (Fe,Cu)-doped SnO{sub 2} the results exhibit that Cu strongly influences on the magnetic properties of these doped systems which are in good agreement with the experimental observations. Electronic structure show that the presence of Cu promote the ferromagnetic bound magnetic polaron interaction through the carriers introduce by d (Cu)

  5. High-resolution gamma-ray spectroscopy with a microwave-multiplexed transition-edge sensor array

    CERN Document Server

    Noroozian, Omid; Bennett, Douglas A; Brevik, Justus A; Fowler, Joseph W; Gao, Jiansong; Hilton, Gene C; Horansky, Robert D; Irwin, Kent D; Kang, Zhao; Schmidt, Daniel R; Vale, Leila R; Ullom, Joel N

    2013-01-01

    We demonstrate very high resolution photon spectroscopy with a microwave-multiplexed two-pixel transition-edge sensor (TES) array. We measured a $^{153}$Gd photon source and achieved an energy resolution of 63 eV full-width-at-half-maximum at 97 keV and an equivalent readout system noise of 86 pA/$\\sqrt{\\text{Hz}}$ at the TES. The readout circuit consists of superconducting microwave resonators coupled to radio-frequency superconducting-quantum-interference-devices (SQUID) and transduces changes in input current to changes in phase of a microwave signal. We use flux-ramp modulation to linearize the response and evade low-frequency noise. This demonstration establishes one path for the readout of cryogenic X-ray and gamma-ray sensor arrays with more than $10^3$ elements and spectral resolving powers $R=\\lambda/\\Delta\\lambda > 10^3$.

  6. [Characteristics of canopy structure of super high yielding japonica hybrid rice community].

    Science.gov (United States)

    Chen, Jinhong; Zhang, Guoping; Guo, Hengde; Mao, Guojuan

    2003-06-01

    In this paper, the characteristics of canopy structure, such as the numbers of seedling, panicle and grain, the distribution of dry matters in different canopy layers and different organs, and the distributions of LAI and of solar radiation in different canopy layers of super high yielding community of japonica hybrid rice were studied, in comparison with normal japonica rice. The results showed that the total the dry matter weight and the dry matter weight of layers below 40 cm, 40-60 cm, 60-80 cm and above 80 cm of japonica hybrid rice canopy were 32.29%, 29.12%, 13.95%, 16.45% and 100.17% higher those that of normal japonica rice, respectively. The ratios of dry leaf (photosynthetic organ) and of dry panicle (sink organ) weight to total dry weight were 24.8% and 12.8%, respectively, which were greater than those of normal japonica rice, while the ratios of dry sheath and stem (storage organs) weight were 33.6% and 28.9%, respectively, which were lower than those of normal japonica rice. The allotment of LAI in different layers of japonica hybrid rice canopy was reasonable, and the LAI of above 40 cm layer at full heading stage reached 5.44. The solar radiation was well-distributed inside japonica hybrid rice canopy, for example, the solar radiation in layers below 60 cm were 13.1%-37.0% higher, but 5.9%-12.2% lower above 60 cm than that of normal japonica rice. The extinction coefficients of solar radiation in layers below 20 cm, 20-40 cm, 40-60 cm and 60-80 cm of japonica hybrid rice canopy were 35.1%, 13.5%, 29.1% and 17.2% lower than that of normal japonica rice, respectively.

  7. Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution.

    Science.gov (United States)

    Meddens, Marjolein B M; Liu, Sheng; Finnegan, Patrick S; Edwards, Thayne L; James, Conrad D; Lidke, Keith A

    2016-06-01

    We have developed a method for performing light-sheet microscopy with a single high numerical aperture lens by integrating reflective side walls into a microfluidic chip. These 45° side walls generate light-sheet illumination by reflecting a vertical light-sheet into the focal plane of the objective. Light-sheet illumination of cells loaded in the channels increases image quality in diffraction limited imaging via reduction of out-of-focus background light. Single molecule super-resolution is also improved by the decreased background resulting in better localization precision and decreased photo-bleaching, leading to more accepted localizations overall and higher quality images. Moreover, 2D and 3D single molecule super-resolution data can be acquired faster by taking advantage of the increased illumination intensities as compared to wide field, in the focused light-sheet.

  8. Three-dimensional super-wideband micro-antenna for high-resolution millimeter-wave medical imaging.

    Science.gov (United States)

    Mirbeik, Amir; Tavassoli, Vahid; Ayazi, Farrokh; Tavassolian, Negar

    2014-01-01

    This paper reports on a novel super-wideband micro-hemispherical antenna with application in millimeter-wave medical imaging. The antenna is composed of a hemispherical shell suspended above a substrate and can be fabricated using a fabrication technology originally developed for micron-scale electromechanical resonators. The antenna exhibits a wide fractional bandwidth of more than 80% (from 64 GHz to 150 GHz) and a high gain of 8.6 dBi at its center frequency. Radiation parameters of the antenna are characterized and the effect of its super-wideband behavior on pulsed millimeter-wave imaging is demonstrated. Finally, a preliminary array configuration composed of two antennas placed side-by-side in the vicinity of a skin-mimicking target is evaluated and the ability to fully detect the target has been demonstrated.

  9. New views of the human NK cell immunological synapse: recent advances enabled by super- and high- resolution imaging techniques

    Directory of Open Access Journals (Sweden)

    Emily M. Mace

    2013-01-01

    Full Text Available Imaging technology has undergone rapid growth with the development of super resolution microscopy, which enables resolution below the diffraction barrier of light (~200 nm. In addition, new techniques for single molecule imaging are being added to the cell biologist’s arsenal. Immunologists have exploited these techniques to advance understanding of NK biology, particularly that of the immune synapse. The immune synapse’s relatively small size and complex architecture combined with its exquisitely controlled signaling milieu have made it a challenge to visualize. In this review we highlight and discuss new insights into NK cell immune synapse formation and regulation revealed by cutting edge imaging techniques, including super resolution microscopy and high resolution total internal reflection microscopy and Förster resonance energy transfer.

  10. Super continuum generation at 800 nm in highly nonlinear photonic crystal fibers with normal dispersion

    DEFF Research Database (Denmark)

    Hansen, Kim Per; Larsen, Jacob Juul; Jensen, Jacob Riis

    2001-01-01

    More than 90 nm broad self-phase modulation (SPM) induced pulses have been created from both 25 and 50 fs pulses in just 12.5 cm of fiber. The broadening is more than 2.5 times that observed in standard SMF. SPM broadening in PCFs has several advantages over more complex super continuum generation...

  11. Practice and Thought on Developing Hybrid Rice for Super High Yield by Exploiting Inter-subspecific Heterosis

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Since the breakthrough of grain yield owing to the development of dwarf rice and three-line system hybrid rice, rice breeding for high yield hardly had showed significant progress in the next successive two decades. It was considered that utilizing heterosis between subspecific varieties (Oryza sativa L.) would be an effective approach to increase yield further. During 1987-1993,an indica-japonica hybrid Yayou 2 yielded as high as 10.5 t/ha; however, it failed to be commercialized because of seed purity problem due to non-uniform emasculation by chemical agent in seed production, and sensitivity of seed setting in F1 plants to environmental conditions. In the past decade, two inter-subspeific hybrids, Liangyoupeijiu (Peiai 64S/9311, javanica/indica) and Liangyou E32 (Peiai 64S/E32, javanica/japonica); both of them exhibited grain yield higher than 10.5 t/ha, and were widely judged as the pioneers of super hybrid rice. Liangyoupeijiu has been successfully popularized over 4 million hectare in wide climatic areas, while Liangyou E32 made a yield record and offered a model of plant ideotype for super hybrid rice. It was considered that in combination with plant ideotype, active physiological functions, and wide-range adaptability to ecological conditions, exploitation of indica-japonica heterosis would be the key approach for super hybrid rice breeding.

  12. Individually addressable microelectrode arrays fabricated with gold-coated pencil graphite particles for multiplexed and high sensitive impedance immunoassays.

    Science.gov (United States)

    Zhang, Yun; Wang, Hua; Nie, Jinfang; Zhang, Yuwei; Shen, Guoli; Yu, Ruqin

    2009-09-15

    A renewable, site-selective immobilization platform of microelectrode array (MEA) for multiplexed immunoassays has been initially developed using pencil graphite particles coated with gold layers as microelectrodes. The graphite particles available on the common pencil were utilized for directing the electro-deposition of gold layers with uniform microstructures which displayed a well-defined sigmoidal voltammetric response. In the concept-of-proof experiments, the resulting MEA platform was modified with functionalized monolayer, on which anti-human IgG antibodies could be stably immobilized in a site-selective way through binding chemistry to selectively capture human IgG antigens from the sample media. The subsequent introduction of anti-human IgG antibodies conjugated with 15 nm electro-active gold nanoparticles to recognize the captured IgG proteins resulted in a significant decrease in the interfacial electron-transfer resistance. High sensitive electrochemical quantification by gold nanoparticle-amplified impedance responses could thus be achieved. Experimental results show that the developed MEA sensor can allow for the detection of human IgG with wide linear range (0.05-100 ng ml(-1)) and sensitivity over 10(3) larger than that of the conventional, bulk gold electrode. The rapid regeneration of the used MEA platform can additionally be realized by a simple electrochemical treatment. The high selectivity of four individually addressable MEA platforms for multiple antigens in a single sample has been further demonstrated in the multiplexed immunoassay experiments. Such a site-selective immobilization strategy of MEA platform may open a new door towards the development of various simple, sensitive, cost-effective, and reusable biological sensors and biochips.

  13. A scheme of optical interconnection for super high speed parallel computer

    Institute of Scientific and Technical Information of China (English)

    Youju Mao(毛幼菊); Yi L(u)(吕翊); Jiang Liu(刘江); Mingrui Dang(党明瑞)

    2004-01-01

    An optical cross connection network which adopts coarse wavelength division multiplexing (CWDM) and data packet is introduced. It can be used to realize communication between multi-CPU and multi-MEM in parallel computing system. It provides an effective way to upgrade the capability of parallel computer by combining optical wavelength division multiplexing (WDM) and data packet switching technology. CWDM used in network construction, optical cross connection (OXC) based on optical switch arrays, and data packet format used in network construction were analyzed. We have also done the optimizing analysis of the number of optical switches needed in different scales of network in this paper. The architecture of the optical interconnection for 8 wavelength channels and 128 bits parallel transmission has been researched. Finally, a parallel transmission system with 4 nodes, 8 channels per node, has been designed.

  14. Analysis of the Key and Difficult Points in the Engineering Construction Technology of the Steel Structures of a Super High-rise Building

    Science.gov (United States)

    Dou, Lijun; Li, Hengxu

    2017-07-01

    In recent years, with the rapid development of the economy of China, the traditional building structure has not been able to meet the current people’s demands and the super high-rise building has become a symbol of a city. In the current period, the research on the super high-rise building in the architectural industry of China is late and the technical blanks exist in some construction difficulties in the super high-rise steel structures. Based on the above, a brief analysis and discussion on the difficult construction technology in the steel structures of a super high-rise building and some measures are presented for reference of the relevant personnel in this paper.

  15. Orbital Angular Momentum-based Space Division Multiplexing for High-capacity Underwater Optical Communications

    Science.gov (United States)

    Ren, Yongxiong; Li, Long; Wang, Zhe; Kamali, Seyedeh Mahsa; Arbabi, Ehsan; Arbabi, Amir; Zhao, Zhe; Xie, Guodong; Cao, Yinwen; Ahmed, Nisar; Yan, Yan; Liu, Cong; Willner, Asher J.; Ashrafi, Solyman; Tur, Moshe; Faraon, Andrei; Willner, Alan E.

    2016-09-01

    To increase system capacity of underwater optical communications, we employ the spatial domain to simultaneously transmit multiple orthogonal spatial beams, each carrying an independent data channel. In this paper, we show up to a 40-Gbit/s link by multiplexing and transmitting four green orbital angular momentum (OAM) beams through a single aperture. Moreover, we investigate the degrading effects of scattering/turbidity, water current, and thermal gradient-induced turbulence, and we find that thermal gradients cause the most distortions and turbidity causes the most loss. We show systems results using two different data generation techniques, one at 1064 nm for 10-Gbit/s/beam and one at 520 nm for 1-Gbit/s/beam; we use both techniques since present data-modulation technologies are faster for infrared (IR) than for green. For the 40-Gbit/s link, data is modulated in the IR, and OAM imprinting is performed in the green using a specially-designed metasurface phase mask. For the 4-Gbit/s link, a green laser diode is directly modulated. Finally, we show that inter-channel crosstalk induced by thermal gradients can be mitigated using multi-channel equalisation processing.

  16. Stand-Sit Microchip for High-Throughput, Multiplexed Analysis of Single Cancer Cells.

    Science.gov (United States)

    Ramirez, Lisa; Herschkowitz, Jason I; Wang, Jun

    2016-01-01

    Cellular heterogeneity in function and response to therapeutics has been a major challenge in cancer treatment. The complex nature of tumor systems calls for the development of advanced multiplexed single-cell tools that can address the heterogeneity issue. However, to date such tools are only available in a laboratory setting and don't have the portability to meet the needs in point-of-care cancer diagnostics. Towards that application, we have developed a portable single-cell system that is comprised of a microchip and an adjustable clamp, so on-chip operation only needs pipetting and adjusting of clamping force. Up to 10 proteins can be quantitated from each cell with hundreds of single-cell assays performed in parallel from one chip operation. We validated the technology and analyzed the oncogenic signatures of cancer stem cells by quantitating both aldehyde dehydrogenase (ALDH) activities and 5 signaling proteins in single MDA-MB-231 breast cancer cells. The technology has also been used to investigate the PI3K pathway activities of brain cancer cells expressing mutant epidermal growth factor receptor (EGFR) after drug intervention targeting EGFR signaling. Our portable single-cell system will potentially have broad application in the preclinical and clinical settings for cancer diagnosis in the future.

  17. Antenna polarization diversity for high-speed polarization multiplexing wireless signal delivery at W-band.

    Science.gov (United States)

    Li, Xinying; Yu, Jianjun; Chi, Nan; Xiao, Jiangnan

    2014-03-01

    We propose and experimentally demonstrate a novel architecture for a W-band integrated optical wireless system, which adopts a 2×2 multiple-input multiple-output (MIMO) wireless link based on antenna polarization diversity, and can realize 80 km single-mode fiber-28 transmission and 2 m wireless delivery for up to 39 Gbaud polarization-division-multiplexing quadrature-phase-shift-keying (PDM-QPSK) signal at 100 GHz. Classic constant-modulus-algorithm (CMA) equalization is adopted at the receiver to implement polarization demultiplexing. The 2×2 MIMO wireless link adopts one pair of horizontal-polarization (H-polarization) horn antennas (HAs) and one pair of vertical-polarization (V-polarization) HAs. Because the two pairs of HAs are fully isolated, the wireless cross talk can be effectively avoided. Thus, compared to the 2×2 MIMO wireless link at the same antenna polarization, the adoption of antenna polarization diversity cannot only make the HA adjustment easier but can also reduce the required CMA tap number. After removing 20% forward-error-correction overhead, the 39 Gbaud baud rate corresponds to a net bit rate of 130  Gb/s, which, to our best knowledge, is the highest bit rate per PDM channel demonstrated for wireless signal delivery up to now.

  18. A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

    Directory of Open Access Journals (Sweden)

    Cécile Beck

    2015-01-01

    Full Text Available West Nile virus (WNV, Japanese encephalitis virus (JEV, and tick-borne encephalitis virus (TBEV are flaviviruses responsible for severe neuroinvasive infections in humans and horses. The confirmation of flavivirus infections is mostly based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs. These tests suffer from poor specificity, mainly due to antigenic cross-reactivity among flavivirus members. Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs which are time-consuming and require BSL3 facilities. The flavivirus envelope (E glycoprotein ectodomain is composed of three domains (D named DI, DII, and DIII, with EDIII containing virus-specific epitopes. In order to improve the serological differentiation of flavivirus infections, the recombinant soluble ectodomain of WNV E (WNV.sE and EDIIIs (rEDIIIs of WNV, JEV, and TBEV were synthesised using the Drosophila S2 expression system. Purified antigens were covalently bonded to fluorescent beads. The microspheres coupled to WNV.sE or rEDIIIs were assayed with about 300 equine immune sera from natural and experimental flavivirus infections and 172 nonimmune equine sera as negative controls. rEDIII-coupled microspheres captured specific antibodies against WNV, TBEV, or JEV in positive horse sera. This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases.

  19. A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

    Science.gov (United States)

    Beck, Cécile; Desprès, Philippe; Paulous, Sylvie; Vanhomwegen, Jessica; Lowenski, Steeve; Nowotny, Norbert; Durand, Benoit; Garnier, Annabelle; Blaise-Boisseau, Sandra; Guitton, Edouard; Yamanaka, Takashi; Zientara, Stéphan; Lecollinet, Sylvie

    2015-01-01

    West Nile virus (WNV), Japanese encephalitis virus (JEV), and tick-borne encephalitis virus (TBEV) are flaviviruses responsible for severe neuroinvasive infections in humans and horses. The confirmation of flavivirus infections is mostly based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs). These tests suffer from poor specificity, mainly due to antigenic cross-reactivity among flavivirus members. Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities. The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes. In order to improve the serological differentiation of flavivirus infections, the recombinant soluble ectodomain of WNV E (WNV.sE) and EDIIIs (rEDIIIs) of WNV, JEV, and TBEV were synthesised using the Drosophila S2 expression system. Purified antigens were covalently bonded to fluorescent beads. The microspheres coupled to WNV.sE or rEDIIIs were assayed with about 300 equine immune sera from natural and experimental flavivirus infections and 172 nonimmune equine sera as negative controls. rEDIII-coupled microspheres captured specific antibodies against WNV, TBEV, or JEV in positive horse sera. This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases. PMID:26457301

  20. Orbital Angular Momentum-based Space Division Multiplexing for High-capacity Underwater Optical Communications

    CERN Document Server

    Ren, Yongxiong; Wang, Zhe; Kamali, Seyedeh Mahsa; Arbabi, Ehsan; Arbabi, Amir; Zhao, Zhe; Xie, Guodong; Cao, Yinwen; Ahmed, Nisar; Yan, Yan; Liu, Cong; Willner, Asher J; Ashrafi, Solyman; Tur, Moshe; Faraon, Andrei; Willner, Alan E

    2016-01-01

    To increase system capacity of underwater optical communications, we employ the spatial domain to simultaneously transmit multiple orthogonal spatial beams, each carrying an independent data channel. In this paper, we multiplex and transmit four green orbital angular momentum (OAM) beams through a single aperture. Moreover, we investigate the degrading effects of scattering/turbidity, water current, and thermal gradient-induced turbulence, and we find that thermal gradients cause the most distortions and turbidity causes the most loss. We show systems results using two different data generation techniques, one at 1064 nm for 10-Gbit/s/beam and one at 520 nm for 1-Gbit/s/beam, we use both techniques since present data-modulation technologies are faster for infrared (IR) than for green. For the higher-rate link, data is modulated in the IR, and OAM imprinting is performed in the green using a specially-designed metasurface phase mask. For the lower rates, a green laser diode is directly modulated. Finally, we s...

  1. Orbital Angular Momentum-based Space Division Multiplexing for High-capacity Underwater Optical Communications

    Science.gov (United States)

    Ren, Yongxiong; Li, Long; Wang, Zhe; Kamali, Seyedeh Mahsa; Arbabi, Ehsan; Arbabi, Amir; Zhao, Zhe; Xie, Guodong; Cao, Yinwen; Ahmed, Nisar; Yan, Yan; Liu, Cong; Willner, Asher J.; Ashrafi, Solyman; Tur, Moshe; Faraon, Andrei; Willner, Alan E.

    2016-01-01

    To increase system capacity of underwater optical communications, we employ the spatial domain to simultaneously transmit multiple orthogonal spatial beams, each carrying an independent data channel. In this paper, we show up to a 40-Gbit/s link by multiplexing and transmitting four green orbital angular momentum (OAM) beams through a single aperture. Moreover, we investigate the degrading effects of scattering/turbidity, water current, and thermal gradient-induced turbulence, and we find that thermal gradients cause the most distortions and turbidity causes the most loss. We show systems results using two different data generation techniques, one at 1064 nm for 10-Gbit/s/beam and one at 520 nm for 1-Gbit/s/beam; we use both techniques since present data-modulation technologies are faster for infrared (IR) than for green. For the 40-Gbit/s link, data is modulated in the IR, and OAM imprinting is performed in the green using a specially-designed metasurface phase mask. For the 4-Gbit/s link, a green laser diode is directly modulated. Finally, we show that inter-channel crosstalk induced by thermal gradients can be mitigated using multi-channel equalisation processing. PMID:27615808

  2. Stand-Sit Microchip for High-Throughput, Multiplexed Analysis of Single Cancer Cells

    Science.gov (United States)

    Ramirez, Lisa; Herschkowitz, Jason I.; Wang, Jun

    2016-01-01

    Cellular heterogeneity in function and response to therapeutics has been a major challenge in cancer treatment. The complex nature of tumor systems calls for the development of advanced multiplexed single-cell tools that can address the heterogeneity issue. However, to date such tools are only available in a laboratory setting and don’t have the portability to meet the needs in point-of-care cancer diagnostics. Towards that application, we have developed a portable single-cell system that is comprised of a microchip and an adjustable clamp, so on-chip operation only needs pipetting and adjusting of clamping force. Up to 10 proteins can be quantitated from each cell with hundreds of single-cell assays performed in parallel from one chip operation. We validated the technology and analyzed the oncogenic signatures of cancer stem cells by quantitating both aldehyde dehydrogenase (ALDH) activities and 5 signaling proteins in single MDA-MB-231 breast cancer cells. The technology has also been used to investigate the PI3K pathway activities of brain cancer cells expressing mutant epidermal growth factor receptor (EGFR) after drug intervention targeting EGFR signaling. Our portable single-cell system will potentially have broad application in the preclinical and clinical settings for cancer diagnosis in the future. PMID:27581736

  3. High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell

    Directory of Open Access Journals (Sweden)

    Qureshi Nasib

    2006-05-01

    Full Text Available Abstract Background The field of plasmid-based functional proteomics requires the rapid assay of proteins expressed from plasmid libraries. Automation is essential since large sets of mutant open reading frames are being cloned for evaluation. To date no integrated automated platform is available to carry out the entire process including production of plasmid libraries, expression of cloned genes, and functional testing of expressed proteins. Results We used a functional proteomic assay in a multiplexed setting on an integrated plasmid-based robotic workcell for high-throughput screening of mutants of cellulase F, an endoglucanase from the anaerobic fungus Orpinomyces PC-2. This allowed us to identify plasmids containing optimized clones expressing mutants with improved activity at lower pH. A plasmid library of mutagenized clones of the celF gene with targeted variations in the last four codons was constructed by site-directed PCR mutagenesis and transformed into Escherichia coli. A robotic picker integrated into the workcell was used to inoculate medium in a 96-well deep well plate, combining the transformants into a multiplexed set in each well, and the plate was incubated on the workcell. Plasmids were prepared from the multiplexed culture on the liquid handler component of the workcell and used for in vitro transcription/translation. The multiplexed expressed recombinant proteins were screened for improved activity and stability in an azo-carboxymethylcellulose plate assay. The multiplexed wells containing mutants with improved activity were identified and linked back to the corresponding multiplexed cultures stored in glycerol. Spread plates were prepared from the glycerol stocks and the workcell was used to pick single colonies from the spread plates, prepare plasmid, produce recombinant protein, and assay for activity. The screening assay and subsequent deconvolution of the multiplexed wells resulted in identification of improved Cel

  4. Super-resolution

    DEFF Research Database (Denmark)

    Nasrollahi, Kamal; Moeslund, Thomas B.

    2014-01-01

    Super-resolution, the process of obtaining one or more high-resolution images from one or more low-resolution observations, has been a very attractive research topic over the last two decades. It has found practical applications in many real world problems in different fields, from satellite...... the contributions of different authors to the basic concepts of each group. Furthermore, common issues in super-resolution algorithms, such as imaging models and registration algorithms, optimization of the cost functions employed, dealing with color information, improvement factors, assessment of super...

  5. Evolution of undissolved phases in high-zinc content super-high strength aluminum alloy during ageing

    Institute of Scientific and Technical Information of China (English)

    张坤; 刘志义; 叶呈武; 许晓嫦; 郑青春

    2004-01-01

    The evolution of undissolved phases in the high-zinc content super-high strength aluminum alloy during ageing was investigated by means of SEM and EIS. The results show that undissolved phases of Cu-rich M(AlZnMgCu) exist in the silver-free alloy at solid-solution state. With increasing the ageing time, the precipitation of agehardening precipitates MgZn2 stimulates Zn atoms within the undissolved phases to diffuse into the matrix, and thus the Cu content in the M(AlZnMgCu) phase increases relatively. For the silver-bearing alloy, small addition of Ag promotes the formation of Ag-rich M(A1ZnMgCuAg) undissolved phases and deteriorates mechanical properties of the alloy. At the early stage of ageing, Ag content within the M(AlZnMgCuAg) phases greatly decreases due to rapid diffusing of Ag atoms into the matrix and the co-clustering of Ag and Mg atoms. As the ageing time prolonging, the precipitation of MgZn2 results in the decrease of Zn content in the undissolved phases, and the relative increase of Ag and Mg contents.

  6. High intensity uranium beams from the superHILAC and the bevatron: final report

    Energy Technology Data Exchange (ETDEWEB)

    1982-03-01

    The two injectors formerly used at the SuperHILAC were a 750-kV air-insulated Cockcroft-Walton (EVE) and a 2.5-MV pressurized HV multiplier (ADAM). The EVE injector can deliver adequate intensities of ions up to mass 40 (argon). The ADAM injector can accelerate ions with lower charge-to-mass ratios, and they can produce beams of heavier ions. The intensity of these beams decreases as the mass number increases, with the lowest practical intensity being achieved with lead beams. Experience with the two existing injectors provided substantial help in defining the general requirements for a new injector which would provide ample beams above mass 40. The requirements for acceptance by the first tank of the SuperHILAC are a particle velocity ..beta.. = 0.0154 (corresponding to an energy of 113 keV/amu) and a charge-to-mass ratio of 0.046 or larger. Present ion source performance dictates an air-insulated Cockcroft-Walton as a pre-accelerator because of its easy accessibility and its good overall reliability. The low charge state ions then receive further acceleration and, if necessary, subsequent stripping to the required charge state before injection into the SuperHILAC. A low-beta linac of the Widereoe type has been built to perform this acceleration. The injector system described consists of a Cockcroft-Walton pre-injector, injection beam lines and isotope analysis, a low-velocity linear accelerator, and SuperHILAC control center modifications.

  7. High-Efficiency Retrofit Lessons for Retail from a SuperTarget: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Langner, R.; Deru, M.; Hirsch, A.; Williams, S.

    2013-02-01

    The National Renewable Energy Laboratory partnered with Target under the Commercial Building Program to design and implement a retrofit of a SuperTarget in Thornton, CO. The result was a retrofit design that predicted 37% energy savings over ASHRAE Standard 90.1-2004, and 29% compared to existing (pre-retrofit) store consumption. The largest savings came from energy efficient lighting, energy efficient cooling systems, improved refrigeration, and better control of plug loads.

  8. Development of a novel depth of interaction PET detector using highly multiplexed G-APD cross-strip encoding

    Energy Technology Data Exchange (ETDEWEB)

    Kolb, A., E-mail: armin.kolb@med.uni-tuebingen.de; Parl, C.; Liu, C. C.; Pichler, B. J. [Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University, 72076 Tübingen (Germany); Mantlik, F. [Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University, 72076 Tübingen, Germany and Department of Empirical Inference, Max Planck Institute for Intelligent Systems, 72076 Tübingen (Germany); Lorenz, E. [Max Planck Institute for Physics, Föhringer Ring 6, 80805 München (Germany); Renker, D. [Department of Physics, Technische Universität München, 85748 Garching (Germany)

    2014-08-15

    Purpose: The aim of this study was to develop a prototype PET detector module for a combined small animal positron emission tomography and magnetic resonance imaging (PET/MRI) system. The most important factor for small animal imaging applications is the detection sensitivity of the PET camera, which can be optimized by utilizing longer scintillation crystals. At the same time, small animal PET systems must yield a high spatial resolution. The measured object is very close to the PET detector because the bore diameter of a high field animal MR scanner is limited. When used in combination with long scintillation crystals, these small-bore PET systems generate parallax errors that ultimately lead to a decreased spatial resolution. Thus, we developed a depth of interaction (DoI) encoding PET detector module that has a uniform spatial resolution across the whole field of view (FOV), high detection sensitivity, compactness, and insensitivity to magnetic fields. Methods: The approach was based on Geiger mode avalanche photodiode (G-APD) detectors with cross-strip encoding. The number of readout channels was reduced by a factor of 36 for the chosen block elements. Two 12 × 2 G-APD strip arrays (25μm cells) were placed perpendicular on each face of a 12 × 12 lutetium oxyorthosilicate crystal block with a crystal size of 1.55 × 1.55 × 20 mm. The strip arrays were multiplexed into two channels and used to calculate the x, y coordinates for each array and the deposited energy. The DoI was measured in step sizes of 1.8 mm by a collimated {sup 18}F source. The coincident resolved time (CRT) was analyzed at all DoI positions by acquiring the waveform for each event and applying a digital leading edge discriminator. Results: All 144 crystals were well resolved in the crystal flood map. The average full width half maximum (FWHM) energy resolution of the detector was 12.8% ± 1.5% with a FWHM CRT of 1.14 ± 0.02 ns. The average FWHM DoI resolution over 12 crystals was 2.90

  9. Development of a novel depth of interaction PET detector using highly multiplexed G-APD cross-strip encoding.

    Science.gov (United States)

    Kolb, A; Parl, C; Mantlik, F; Liu, C C; Lorenz, E; Renker, D; Pichler, B J

    2014-08-01

    The aim of this study was to develop a prototype PET detector module for a combined small animal positron emission tomography and magnetic resonance imaging (PET/MRI) system. The most important factor for small animal imaging applications is the detection sensitivity of the PET camera, which can be optimized by utilizing longer scintillation crystals. At the same time, small animal PET systems must yield a high spatial resolution. The measured object is very close to the PET detector because the bore diameter of a high field animal MR scanner is limited. When used in combination with long scintillation crystals, these small-bore PET systems generate parallax errors that ultimately lead to a decreased spatial resolution. Thus, we developed a depth of interaction (DoI) encoding PET detector module that has a uniform spatial resolution across the whole field of view (FOV), high detection sensitivity, compactness, and insensitivity to magnetic fields. The approach was based on Geiger mode avalanche photodiode (G-APD) detectors with cross-strip encoding. The number of readout channels was reduced by a factor of 36 for the chosen block elements. Two 12 × 2 G-APD strip arrays (25 μm cells) were placed perpendicular on each face of a 12 × 12 lutetium oxyorthosilicate crystal block with a crystal size of 1.55 × 1.55 × 20 mm. The strip arrays were multiplexed into two channels and used to calculate the x, y coordinates for each array and the deposited energy. The DoI was measured in step sizes of 1.8 mm by a collimated (18)F source. The coincident resolved time (CRT) was analyzed at all DoI positions by acquiring the waveform for each event and applying a digital leading edge discriminator. All 144 crystals were well resolved in the crystal flood map. The average full width half maximum (FWHM) energy resolution of the detector was 12.8% ± 1.5% with a FWHM CRT of 1.14 ± 0.02 ns. The average FWHM DoI resolution over 12 crystals was 2.90 ± 0.15 mm. The novel DoI PET

  10. Determination of ANA specificity using multiplexed fluorescent microsphere immunoassay in patients with ANA positivity at high titres after infliximab treatment: preliminary results.

    Science.gov (United States)

    Caramaschi, Paola; Ruzzenente, Orazio; Pieropan, Sara; Volpe, Alessandro; Carletto, Antonio; Bambara, Lisa Maria; Biasi, Domenico

    2007-05-01

    To evaluate ANA specificity using the fully automated multiplexed fluorescent microsphere immunoassay in patients affected either by rheumatoid arthritis or ankylosing spondylitis who developed strong positivity for ANA as assessed by indirect immunofluorescent method on HEp-2 cells during infliximab treatment. Three men affected by ankylosing spondylitis and 12 women affected by rheumatoid arthritis who developed ANA positivity at high titres during infliximab treatment underwent the identification of ANA specificity by multiplexed fluorescent microsphere immunoassay; moreover anti-DNA and anti-ENA antibodies were tested by indirect immunofluorescence and ELISA method, respectively. In 4 out of 15 cases, the determination of ANA reactivity by multiplexed fluorescent microsphere immunoassay was also performed on the serum collected before infliximab administration. One patient affected by rheumatoid arthritis showed multiple ANA reactivities against SS-A, SS-B, RNP, Sm, Jo-1 and histones; one patient affected by ankylosing spondylitis resulted positive for the same autoantibodies, except for anti-Sm antibody. Moreover, two patients, one with rheumatoid arthritis and one with ankylosing spondylitis, showed single antibody specificity to SS-B and RNP, respectively. The remaining 11 cases did not show any positivity. Instead, all the patients resulted negative for anti-ENA antibodies by the ELISA method. In the four cases tested for ANA specificity by multiplexed fluorescent microsphere immunoassay before and after infliximab administration no difference was found. The search for anti-DNA antibody always resulted negative by both the traditional immunofluorescent assay and the novel technique. The use of multiplexed fluorescent microsphere immunoassay in patients treated with infliximab with ANA positivity at high titres allowed to find some ANA specificities which were not revealed by ELISA method. Nevertheless, the majority of patients resulted negative in spite of

  11. Silicon Nanowire Biosensor for Highly Sensitive and Multiplexed Detection of Oral Squamous Cell Carcinoma Biomarkers in Saliva

    National Research Council Canada - National Science Library

    ZHANG, Yulin; CHEN, Rongmei; XU, Lu; NING, Yong; XIE, Shenggao; ZHANG, Guo-Jun

    2015-01-01

    .... Subsequently, we achieved the multiplexed detection of two biomarkers in saliva by specific recognition of the two biomarkers with their corresponding antibodies, which were modified on the SiNW...

  12. Super-resolution

    DEFF Research Database (Denmark)

    Nasrollahi, Kamal; Moeslund, Thomas B.

    2014-01-01

    and aerial imaging to medical image processing, to facial image analysis, text image analysis, sign and number plates reading, and biometrics recognition, to name a few. This has resulted in many research papers, each developing a new super-resolution algorithm for a specific purpose. The current......Super-resolution, the process of obtaining one or more high-resolution images from one or more low-resolution observations, has been a very attractive research topic over the last two decades. It has found practical applications in many real world problems in different fields, from satellite...... the contributions of different authors to the basic concepts of each group. Furthermore, common issues in super-resolution algorithms, such as imaging models and registration algorithms, optimization of the cost functions employed, dealing with color information, improvement factors, assessment of super...

  13. Development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five Brucella species.

    Science.gov (United States)

    Gopaul, Krishna K; Sells, Jessica; Lee, Robin; Beckstrom-Sternberg, Stephen M; Foster, Jeffrey T; Whatmore, Adrian M

    2014-12-11

    The zoonosis brucellosis causes economically significant reproductive problems in livestock and potentially debilitating disease of humans. Although the causative agent, organisms from the genus Brucella, can be differentiated into a number of species based on phenotypic characteristics, there are also significant differences in genotype that are concordant with individual species. This paper describes the development of a five target multiplex assay to identify five terrestrial Brucella species using real-time polymerase chain reaction (PCR) and subsequent high resolution melt curve analysis. This technology offers a robust and cost effective alternative to previously described hydrolysis-probe Single Nucleotide Polymorphism (SNP)-based species defining assays. Through the use of Brucella whole genome sequencing five species defining SNPs were identified. Individual HRM assays were developed to these target these changes and, following optimisation of primer concentrations, it was possible to multiplex all five assays in a single tube. In a validation exercise using a panel of 135 Brucella strains of terrestrial and marine origin, it was possible to distinguish the five target species from the other species within this panel. The HRM multiplex offers a number of diagnostic advantages over previously described SNP-based typing approaches. Further, and uniquely for HRM, the successful multiplexing of five assays in a single tube allowing differentiation of five Brucella species in the diagnostic laboratory in a cost-effective and timely manner is described. However there are possible limitations to using this platform on DNA extractions direct from clinical material.

  14. A low-cost, multiplexed μECoG system for high-density recordings in freely moving rodents

    Science.gov (United States)

    Insanally, Michele; Trumpis, Michael; Wang, Charles; Chiang, Chia-Han; Woods, Virginia; Palopoli-Trojani, Kay; Bossi, Silvia; Froemke, Robert C.; Viventi, Jonathan

    2016-04-01

    Objective. Micro-electrocorticography (μECoG) offers a minimally invasive neural interface with high spatial resolution over large areas of cortex. However, electrode arrays with many contacts that are individually wired to external recording systems are cumbersome and make recordings in freely behaving rodents challenging. We report a novel high-density 60-electrode system for μECoG recording in freely moving rats. Approach. Multiplexed headstages overcome the problem of wiring complexity by combining signals from many electrodes to a smaller number of connections. We have developed a low-cost, multiplexed recording system with 60 contacts at 406 μm spacing. We characterized the quality of the electrode signals using multiple metrics that tracked spatial variation, evoked-response detectability, and decoding value. Performance of the system was validated both in anesthetized animals and freely moving awake animals. Main results. We recorded μECoG signals over the primary auditory cortex, measuring responses to acoustic stimuli across all channels. Single-trial responses had high signal-to-noise ratios (SNR) (up to 25 dB under anesthesia), and were used to rapidly measure network topography within ∼10 s by constructing all single-channel receptive fields in parallel. We characterized evoked potential amplitudes and spatial correlations across the array in the anesthetized and awake animals. Recording quality in awake animals was stable for at least 30 days. Finally, we used these responses to accurately decode auditory stimuli on single trials. Significance. This study introduces (1) a μECoG recording system based on practical hardware design and (2) a rigorous analytical method for characterizing the signal characteristics of μECoG electrode arrays. This methodology can be applied to evaluate the fidelity and lifetime of any μECoG electrode array. Our μECoG-based recording system is accessible and will be useful for studies of perception and decision

  15. Noise evaluation of silicon strip super-module with ABCN250 readout chips for the ATLAS detector upgrade at the High Luminosity LHC

    Energy Technology Data Exchange (ETDEWEB)

    Todome, K., E-mail: todome@hep.phys.titech.ac.jp [Department of Physics, Tokyo Institute of Technology, Ookayama 2-12-1, Meguro-ku, Tokyo 152-8551 (Japan); Solid State Div., Hamamatsu Photonics K.K., 1126-1, Ichino-cho, Higashi-ku, Hamamatsu-shi, Shizuoka 435-8558 (Japan); Jinnouchi, O. [Department of Physics, Tokyo Institute of Technology, Ookayama 2-12-1, Meguro-ku, Tokyo 152-8551 (Japan); Solid State Div., Hamamatsu Photonics K.K., 1126-1, Ichino-cho, Higashi-ku, Hamamatsu-shi, Shizuoka 435-8558 (Japan); Clark, A.; Barbier, G.; Cadoux, F.; Favre, Y.; Ferrere, D.; Gonzalez-Sevilla, S.; Iacobucci, G.; La Marra, D.; Perrin, E.; Weber, M. [DPNC, University of Geneva, CH-1211 Geneva 4 (Switzerland); Solid State Div., Hamamatsu Photonics K.K., 1126-1, Ichino-cho, Higashi-ku, Hamamatsu-shi, Shizuoka 435-8558 (Japan); Ikegami, Y.; Nakamura, K.; Takubo, Y.; Unno, Y. [Institute of Particle and Nuclear Study, KEK, Oho 1-1, Tsukuba, Ibaraki 305-0801 (Japan); Solid State Div., Hamamatsu Photonics K.K., 1126-1, Ichino-cho, Higashi-ku, Hamamatsu-shi, Shizuoka 435-8558 (Japan); Takashima, R. [Department of Science Education, Kyoto University of Education, Kyoto 612-8522 (Japan); Solid State Div., Hamamatsu Photonics K.K., 1126-1, Ichino-cho, Higashi-ku, Hamamatsu-shi, Shizuoka 435-8558 (Japan); Tojo, J. [Department of Physics, Faculty of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395 (Japan); Solid State Div., Hamamatsu Photonics K.K., 1126-1, Ichino-cho, Higashi-ku, Hamamatsu-shi, Shizuoka 435-8558 (Japan); Kono, T. [Ochadai Academic Production, Ochanomizu University, 2-1-1, Otsuka, Bunkyo-ku, Tokyo 112-8610 (Japan); Solid State Div., Hamamatsu Photonics K.K., 1126-1, Ichino-cho, Higashi-ku, Hamamatsu-shi, Shizuoka 435-8558 (Japan); and others

    2016-09-21

    Toward High Luminosity LHC (HL-LHC), the whole ATLAS inner tracker will be replaced, including the semiconductor tracker (SCT) which is the silicon micro strip detector for tracking charged particles. In development of the SCT, integration of the detector is the important issue. One of the concepts of integration is the “super-module” in which individual modules are assembled to produce the SCT ladder. A super-module prototype has been developed to demonstrate its functionality. One of the concerns in integrating the super-modules is the electrical coupling between each module, because it may increase intrinsic noise of the system. To investigate the electrical performance of the prototype, the new Data Acquisition (DAQ) system has been developed by using SEABAS. The electric performance of the super-module prototype, especially the input noise and random noise hit rate, was investigated by using SEABAS system.

  16. Development of A Super High Speed Permanent Magnet Synchronous Motor (PMSM Controller and Analysis of The Experimental Results

    Directory of Open Access Journals (Sweden)

    Limei Zhao

    2005-02-01

    Full Text Available This paper presents the design and implementation of a DSP-based controller for a super high-speed (>80,000 rpm permanent magnet synchronous motor (PMSM. The PMSM is a key component of the centrifugal compressor drive of a reverse Brayton cryocooler that is currently under development for NASA and Florida Solar Energy Center. The design of the PMSM open-loop control is presented. Experimental results with open-loop control schemes are presented. System optimization and analysis are also illustrated. They verify the effectiveness of the controller design and the optimization scheme.

  17. SuperTIGER-2: A Very-Large-Area, High-Resolution Trans-Iron Cosmic Ray Investigation

    Science.gov (United States)

    Binns, Walter

    This is the lead proposal of a multi-institution proposal for the investigation titled “SuperTIGER-2: A Very-Large-Area, High Resolution Trans-Iron Cosmic Ray Investigation”. SuperTIGER is a very-large-area instrument for measuring the composition of galactic cosmic rays on high-altitude balloon flights over Antarctica. SuperTIGER-1 had a highly successful 55-day flight in 2012/2013. The data quality is excellent, enabling us to clearly resolve all nuclei from Z=10 to Z=40. Additionally, although statistics are low, there appears to be clear resolution of elements in the Z=40- 60 range. The excellent data from this flight will enable us to achieve the initial goals of the program. The high performance of the instrument, both in charge resolution and collecting power, and the science that can be addressed by measurements of nuclei heavier than Z=40, makes a compelling case to conduct additional flights to measure the abundances of individual nuclei up to Z=60. This is a 4-year proposal with the primary objective of measuring the abundances of individual nuclei with 41#Z#60 and to substantially increase the number of 30#Z#40 nuclei measured. This will be the first time that individual elemental abundances for the 41#Z#60 range will be obtained. These new measurements will provide sensitive tests and clarification of the OB-association model of galactic cosmic-ray origins and will test models for atomic processes by which nuclei are selected for acceleration to cosmic-ray energies. They will enable us to determine if the enrichment of refractory elements (those that exist primarily in dust grains in the interstellar medium) over volatile elements (those that exist primarily in the gas phase) extends into the Z=41-60 charge range. They will also enable us to unambiguously determine the extent of any r-process enrichment. SuperTIGER provides critical measurements to unravel the mystery of galactic cosmic ray (GCR) origins and complements instruments with different

  18. Construction and Use of Resting 12-Lead High Fidelity ECG "SuperScores" in Screening for Heart Disease

    Science.gov (United States)

    Schlegel, T. T.; Arenare, B.; Greco, E. C.; DePalma, J. L.; Starc, V.; Nunez, T.; Medina, R.; Jugo, D.; Rahman, M.A.; Delgado, R.

    2007-01-01

    We investigated the accuracy of several conventional and advanced resting ECG parameters for identifying obstructive coronary artery disease (CAD) and cardiomyopathy (CM). Advanced high-fidelity 12-lead ECG tests (approx. 5-min supine) were first performed on a "training set" of 99 individuals: 33 with ischemic or dilated CM and low ejection fraction (EF less than 40%); 33 with catheterization-proven obstructive CAD but normal EF; and 33 age-/gender-matched healthy controls. Multiple conventional and advanced ECG parameters were studied for their individual and combined retrospective accuracies in detecting underlying disease, the advanced parameters falling within the following categories: 1) Signal averaged ECG, including 12-lead high frequency QRS (150-250 Hz) plus multiple filtered and unfiltered parameters from the derived Frank leads; 2) 12-lead P, QRS and T-wave morphology via singular value decomposition (SVD) plus signal averaging; 3) Multichannel (12-lead, derived Frank lead, SVD lead) beat-to-beat QT interval variability; 4) Spatial ventricular gradient (and gradient component) variability; and 5) Heart rate variability. Several multiparameter ECG SuperScores were derivable, using stepwise and then generalized additive logistic modeling, that each had 100% retrospective accuracy in detecting underlying CM or CAD. The performance of these same SuperScores was then prospectively evaluated using a test set of another 120 individuals (40 new individuals in each of the CM, CAD and control groups, respectively). All 12-lead ECG SuperScores retrospectively generated for CM continued to perform well in prospectively identifying CM (i.e., areas under the ROC curve greater than 0.95), with one such score (containing just 4 components) maintaining 100% prospective accuracy. SuperScores retrospectively generated for CAD performed somewhat less accurately, with prospective areas under the ROC curve typically in the 0.90-0.95 range. We conclude that resting 12-lead

  19. SuperDARN CUTLASS Finland radar observations of high-latitude magnetic reconnections under northward interplanetary magnetic field (IMF) conditions

    Institute of Scientific and Technical Information of China (English)

    ZHANG QingHe; LIU RuiYuan; YANG HuiGen; HU HongQiao; ZHANG BeiChen; DUNLOP Malcolm; LESTER Mark; BOGDANOVA Yulia; WALSH Andrew

    2012-01-01

    A number of backscatter power enhancement events with “equatorward-moving radar auroral forms” in the high-latitude ionosphere were observed by SuperDARN CUTLASS Finland radar when the IMF was northward during 09:00 -10:00 UT on 26 March 2004.These events were also associated with sunward flow enhancements at each location in the Northern Hemisphere which were shown in ionospheric convections measured by the SuperDARN radars.These are typical features of high-latitude (lobe) magnetic reconnections.The durations of the velocity enhancements imply that the evolution time of the lobe reconnections is about 8-16 min from their origin at the reconnection site to their addition to the magnetotail lobe again.In additional,the Double Star TC-1 spacecraft was moving from magnetosheath into magnetosphere,and crossing the magnetopause near the subsolar region during this interval,and observed typical low-latitude magnetic reconnection signatures.This infers that the dayside high- and low-latitude reconnections may occur simultaneously.

  20. Super Special Codes using Super Matrices

    CERN Document Server

    Kandasamy, W B Vasantha; Ilanthenral, K

    2010-01-01

    The new classes of super special codes are constructed in this book using the specially constructed super special vector spaces. These codes mainly use the super matrices. These codes can be realized as a special type of concatenated codes. This book has four chapters. In chapter one basic properties of codes and super matrices are given. A new type of super special vector space is constructed in chapter two of this book. Three new classes of super special codes namely, super special row code, super special column code and super special codes are introduced in chapter three. Applications of these codes are given in the final chapter.

  1. Super Fuzzy Matrices and Super Fuzzy Models for Social Scientists

    CERN Document Server

    Kandasamy, W B Vasantha; Amal, K

    2008-01-01

    This book introduces the concept of fuzzy super matrices and operations on them. This book will be highly useful to social scientists who wish to work with multi-expert models. Super fuzzy models using Fuzzy Cognitive Maps, Fuzzy Relational Maps, Bidirectional Associative Memories and Fuzzy Associative Memories are defined here. The authors introduce 13 multi-expert models using the notion of fuzzy supermatrices. These models are described with illustrative examples. This book has three chapters. In the first chaper, the basic concepts about super matrices and fuzzy super matrices are recalled. Chapter two introduces the notion of fuzzy super matrices adn their properties. The final chapter introduces many super fuzzy multi expert models.

  2. The case for super-critical accretion on massive black holes at high redshift

    CERN Document Server

    Volonteri, Marta

    2014-01-01

    Short-lived intermittent phases of super-critical (super-Eddington) growth, coupled with star formation via feedback, may account for early growth of massive black holes (MBH) and coevolution with their host spheroids. We estimate the possible growth rates and duty cycles of these episodes, assuming slim disk solutions, where the emerging luminosity depends logarithmically on the accretion rate. As long as radiation is trapped in the disk, the effective radiative efficiency is low and several short episodes (~1e4 years) are possible, especially if energy flows through collimated funnels and feedback is limited. When the host galaxy is able to sustain inflow rates at 1-100 msun/yr, replenishing and circulation lead to a sequence of short episodes that may increase the growth rates of MBHs, with respect to an Eddington-limited case, by several orders of magnitude in ~ 1e7 years. Our model for e MBH growth predicts that the ratio of MBH accretion rate to star formation rate is 1e-2 or higher, naturally leading t...

  3. A joint compressed-sensing and super-resolution approach for very high-resolution diffusion imaging.

    Science.gov (United States)

    Ning, Lipeng; Setsompop, Kawin; Michailovich, Oleg; Makris, Nikos; Shenton, Martha E; Westin, Carl-Fredrik; Rathi, Yogesh

    2016-01-15

    Diffusion MRI (dMRI) can provide invaluable information about the structure of different tissue types in the brain. Standard dMRI acquisitions facilitate a proper analysis (e.g. tracing) of medium-to-large white matter bundles. However, smaller fiber bundles connecting very small cortical or sub-cortical regions cannot be traced accurately in images with large voxel sizes. Yet, the ability to trace such fiber bundles is critical for several applications such as deep brain stimulation and neurosurgery. In this work, we propose a novel acquisition and reconstruction scheme for obtaining high spatial resolution dMRI images using multiple low resolution (LR) images, which is effective in reducing acquisition time while improving the signal-to-noise ratio (SNR). The proposed method called compressed-sensing super resolution reconstruction (CS-SRR), uses multiple overlapping thick-slice dMRI volumes that are under-sampled in q-space to reconstruct diffusion signal with complex orientations. The proposed method combines the twin concepts of compressed sensing and super-resolution to model the diffusion signal (at a given b-value) in a basis of spherical ridgelets with total-variation (TV) regularization to account for signal correlation in neighboring voxels. A computationally efficient algorithm based on the alternating direction method of multipliers (ADMM) is introduced for solving the CS-SRR problem. The performance of the proposed method is quantitatively evaluated on several in-vivo human data sets including a true SRR scenario. Our experimental results demonstrate that the proposed method can be used for reconstructing sub-millimeter super resolution dMRI data with very good data fidelity in clinically feasible acquisition time.

  4. High-throughput multiplex quantitative polymerase chain reaction method for Giardia lamblia and Cryptosporidium species detection in stool samples.

    Science.gov (United States)

    Nurminen, Noora; Juuti, Rosa; Oikarinen, Sami; Fan, Yue-Mei; Lehto, Kirsi-Maarit; Mangani, Charles; Maleta, Kenneth; Ashorn, Per; Hyöty, Heikki

    2015-06-01

    Giardia lamblia and Cryptosporidium species belong to a complex group of pathogens that cause diseases hampering development and socioeconomic improvements in the developing countries. Both pathogens are recognized as significant causes of diarrhea and nutritional disorders. However, further studies are needed to clarify the role of parasitic infections, especially asymptomatic infections in malnutrition and stunting. We developed a high-throughput multiplex quantitative polymerase chain reaction (qPCR) method for G. lamblia and Cryptosporidium spp. detection in stool samples. The sensitivity and specificity of the method were ensured by analyzing confirmed positive samples acquired from diagnostics laboratories and participating in an external quality control round. Its capability to detect asymptomatic G. lamblia and Cryptosporidium spp. infections was confirmed by analyzing stool samples collected from 44 asymptomatic 6-month-old infants living in an endemic region in Malawi. Of these, five samples were found to be positive for G. lamblia and two for Cryptosporidium spp. In conclusion, the developed method is suitable for large-scale studies evaluating the occurrence of G. lamblia and Cryptosporidium spp. in endemic regions and for clinical diagnostics of these infections.

  5. Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes.

    Science.gov (United States)

    Bray, Mark-Anthony; Singh, Shantanu; Han, Han; Davis, Chadwick T; Borgeson, Blake; Hartland, Cathy; Kost-Alimova, Maria; Gustafsdottir, Sigrun M; Gibson, Christopher C; Carpenter, Anne E

    2016-09-01

    In morphological profiling, quantitative data are extracted from microscopy images of cells to identify biologically relevant similarities and differences among samples based on these profiles. This protocol describes the design and execution of experiments using Cell Painting, which is a morphological profiling assay that multiplexes six fluorescent dyes, imaged in five channels, to reveal eight broadly relevant cellular components or organelles. Cells are plated in multiwell plates, perturbed with the treatments to be tested, stained, fixed, and imaged on a high-throughput microscope. Next, an automated image analysis software identifies individual cells and measures ∼1,500 morphological features (various measures of size, shape, texture, intensity, and so on) to produce a rich profile that is suitable for the detection of subtle phenotypes. Profiles of cell populations treated with different experimental perturbations can be compared to suit many goals, such as identifying the phenotypic impact of chemical or genetic perturbations, grouping compounds and/or genes into functional pathways, and identifying signatures of disease. Cell culture and image acquisition takes 2 weeks; feature extraction and data analysis take an additional 1-2 weeks.

  6. Differentiation of Campylobacter jejuni and Campylobacter coli Using Multiplex-PCR and High Resolution Melt Curve Analysis.

    Science.gov (United States)

    Banowary, Banya; Dang, Van Tuan; Sarker, Subir; Connolly, Joanne H; Chenu, Jeremy; Groves, Peter; Ayton, Michelle; Raidal, Shane; Devi, Aruna; Vanniasinkam, Thiru; Ghorashi, Seyed A

    2015-01-01

    Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. Among Campylobacter spp. Campylobacter jejuni (C. jejuni) and C. coli are the most common causes of human infection. In this study, a multiplex PCR (mPCR) and high resolution melt (HRM) curve analysis were optimized for simultaneous detection and differentiation of C. jejuni and C. coli isolates. A segment of the hippuricase gene (hipO) of C. jejuni and putative aspartokinase (asp) gene of C. coli were amplified from 26 Campylobacter isolates and amplicons were subjected to HRM curve analysis. The mPCR-HRM was able to differentiate between C. jejuni and C. coli species. All DNA amplicons generated by mPCR were sequenced. Analysis of the nucleotide sequences from each isolate revealed that the HRM curves were correlated with the nucleotide sequences of the amplicons. Minor variation in melting point temperatures of C. coli or C. jejuni isolates was also observed and enabled some intraspecies differentiation between C. coli and/or C. jejuni isolates. The potential of PCR-HRM curve analysis for the detection and speciation of Campylobacter in additional human clinical specimens and chicken swab samples was also confirmed. The sensitivity and specificity of the test were found to be 100% and 92%, respectively. The results indicated that mPCR followed by HRM curve analysis provides a rapid (8 hours) technique for differentiation between C. jejuni and C. coli isolates.

  7. Molecular differentiation of Opisthorchis viverrini and Clonorchis sinensis eggs by multiplex real-time PCR with high resolution melting analysis.

    Science.gov (United States)

    Kaewkong, Worasak; Intapan, Pewpan M; Sanpool, Oranuch; Janwan, Penchom; Thanchomnang, Tongjit; Laummaunwai, Porntip; Lulitanond, Viraphong; Doanh, Pham Ngoc; Maleewong, Wanchai

    2013-12-01

    Opisthorchis viverrini and Clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in Asia. The standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. However, the method has low sensitivity, and eggs of O. viverrini and C. sinensis are difficult to distinguish from each other and from those of some other trematodes. Here, we report a multiplex real-time PCR coupled with high resolution melting (HRM) analysis for the differentiation of O. viverrini and C. sinensis eggs in fecal samples. Using 2 pairs of species-specific primers, DNA sequences from a portion of the mitochondrial NADH dehydrogenase subunit 2 (nad 2) gene, were amplified to generate 209 and 165 bp products for O. viverrini and C. sinensis, respectively. The distinct characteristics of HRM patterns were analyzed, and the melting temperatures peaked at 82.4±0.09℃ and 85.9±0.08℃ for O. viverrini and C. sinensis, respectively. This technique was able to detect as few as 1 egg of O. viverrini and 2 eggs of C. sinensis in a 150 mg fecal sample, which is equivalent to 7 and 14 eggs per gram of feces, respectively. The method is species-specific, rapid, simple, and does not require fluorescent probes or post-PCR processing for discrimination of eggs of the 2 species. It offers a new tool for differentiation and detection of Asian liver fluke infections in stool specimens.

  8. Addressing Decadal Survey Science through Community Access to Highly Multiplexed Spectroscopy with BigBOSS on the KPNO Mayall Telescope

    CERN Document Server

    Pilachowski, Caty; Bailey, Stephen; Barth, Aaron; Beaton, Rachel; Bell, Eric; Bernstein, Rebecca; Bian, Fuyan; Blanton, Michael; Blum, Robert; Bolton, Adam; Bond, Howard; Brodwin, Mark; Bullock, James; Carlin, Jeff; Chary, Ranga-Ram; Cinabro, David; Cooper, Michael; Cota, Jorge L C; Davis, Marc; Dawson, Kyle; Dey, Arjun; Donahue, Megan; Drake, Jeremy; Ellingson, Erica; Faccioli, Lorenzo; Fan, Xiaohui; Ferguson, Harry; Gawiser, Eric; Geha, Marla; Giavalisco, Mauro; Gonzalez, Anthony; Griest, Kim; Grossan, Bruce; Guhathakurta, Raja; Harding, Paul; Heap, Sara R; Ho, Shirley; Howell, Steve; Jannuzi, Buell; Kalirai, Jason; Keeney, Brian; Kewley, Lisa; Kong, Xu; Lampton, Michael; Lin, Wei-Peng; de la Macorra, Axel; Macri, Lucas; Majewski, Steve; Martini, Paul; Massey, Phil; McSwain, Virginia; Miller, Adam A; Minniti, Dante; Modjaz, Maryam; Morrison, Heather; Moustakas, John; Myers, Adam; Najita, Joan; Newman, Jeffrey; Norman, Dara; Olsen, Knut; Pierce, Michael; Pope, Alexandra; Prescott, Moire; Reddy, Naveen; Reil, Kevin; Rest, Armin; Rhode, Katherine; Rockosi, Connie; Rudnick, Greg; Saha, Abhijit; Salzer, John; Sanders, David; Schlegel, David; Sesar, Branimir; Shields, Joseph; Silverman, Jeffrey; Simon, Josh; Stanford, Adam; Stern, Daniel; Storrie-Lombardi, Lisa; Suntzeff, Nicholas; Surace, Jason; Szalay, Alex; Ulmer, Melville; Weiner, Ben; Willman, Beth; Windhorst, Rogier; Wood-Vasey, Michael

    2012-01-01

    This document summarizes the results of a community-based discussion of the potential science impact of the Mayall+BigBOSS highly multiplexed multi-object spectroscopic capability. The KPNO Mayall 4m telescope equipped with the DOE- and internationally-funded BigBOSS spectrograph offers one of the most cost-efficient ways of accomplishing many of the pressing scientific goals identified for this decade by the "New Worlds, New Horizons" report. The BigBOSS Key Project will place unprecedented constraints on cosmological parameters related to the expansion history of the universe. With the addition of an open (publicly funded) community access component, the scientific impact of BigBOSS can be extended to many important astrophysical questions related to the origin and evolution of galaxies, stars, and the IGM. Massive spectroscopy is the critical missing ingredient in numerous ongoing and planned ground- and space-based surveys, and BigBOSS is unique in its ability to provide this to the US community. BigBOSS ...

  9. Super High Dosing with a Novel Buttiauxella Phytase Continuously Improves Growth Performance, Nutrient Digestibility, and Mineral Status of Weaned Pigs.

    Science.gov (United States)

    Zeng, Zhikai; Li, Qingyun; Tian, Qiyu; Zhao, Panfeng; Xu, Xiao; Yu, Shukun; Piao, Xiangshu

    2015-11-01

    This study was conducted to evaluate the efficacy of a novel Buttiauxella phytase to pigs fed P-deficient, corn-soybean meal diets. One hundred and twenty crossbred piglets (9.53 ± 0.84 kg) were allocated to one of five treatments which consisted of four low P diets (0.61 % Ca and 0.46 % total P) supplemented with 0, 500, 1,000, or 20,000 FTU/kg phytase as well as a positive control diet (0.77 % Ca and 0.62 % total P). Each treatment had six replicated pens with four pigs per pen. Pigs were fed the experimental diets for 28 days. Phytase supplementation linearly improved (P phytase (20,000 FTU/kg) further increased (P phytase inclusion group, as well as ATTD of Ca and P. Metacarpal bone characteristics and several trace mineral concentration in bone, plasma, or organ tissues were linearly (P phytase. Super high dosing with phytase (20,000 FTU/kg) supplementation improved (P phytase supplementation (500 or 1,000 FTU/kg). In conclusion, supplementation of 500 FTU of Buttiauxella phytase/kg and above effectively hydrolyzed phytate in a low-P corn-soybean diet for pigs. In addition, a super high dosing with phytase (20,000 FTU/kg) improved macro- or micro mineral availability and growth performance.

  10. OptoCeramic-Based High Speed Fiber Multiplexer for Multimode Fiber Project

    Data.gov (United States)

    National Aeronautics and Space Administration — A fiber-based fixed-array laser transmitter can be combined with a fiber-arrayed detector to create the next-generation NASA array LIDAR systems. High speed optical...

  11. The use of multiplex PCR for the diagnosis of viral severe acute respiratory infection in children: a high rate of co-detection during the winter season.

    Science.gov (United States)

    El Kholy, A A; Mostafa, N A; Ali, A A; Soliman, M M S; El-Sherbini, S A; Ismail, R I; El Basha, N; Magdy, R I; El Rifai, N; Hamed, D H

    2016-10-01

    Respiratory tract infection is a major cause of hospitalization in children. Although most such infections are viral in origin, it is difficult to differentiate bacterial and viral infections, as the clinical symptoms are similar. Multiplex polymerase chain reaction (PCR) methods allow testing for multiple pathogens simultaneously and are, therefore, gaining interest. This prospective case-control study was conducted from October 2013 to February 2014. Nasopharyngeal (NP) and oropharyngeal (throat) swabs were obtained from children admitted with severe acute respiratory infection (SARI) at a tertiary hospital. A control group of 40 asymptomatic children was included. Testing for 16 viruses was done by real-time multiplex PCR. Multiplex PCR detected a viral pathogen in 159/177 (89.9 %) patients admitted with SARI. There was a high rate of co-infection (46.9 %). Dual detections were observed in 64 (36.2 %), triple detections in 17 (9.6 %), and quadruple detections in 2 (1.1 %) of 177 samples. Seventy-eight patients required intensive care unit (ICU) admission, of whom 28 (35.8 %) had co-infection with multiple viruses. AdV, HBoV, HRV, HEV, and HCoV-OC43 were also detected among asymptomatic children. This study confirms the high rate of detection of viral nucleic acids by multiplex PCR among hospitalized children admitted with SARI, as well as the high rate of co-detection of multiple viruses. AdV, HBoV, HRV, HEV, and HCoV-OC43 were also detected in asymptomatic children, resulting in challenges in clinical interpretation. Studies are required to provide quantitative conclusions that will facilitate clinical interpretation and application of the results in the clinical setting.

  12. High-throughput screen for the chemical inhibitors of antiapoptotic bcl-2 family proteins by multiplex flow cytometry.

    Science.gov (United States)

    Curpan, Ramona F; Simons, Peter C; Zhai, Dayong; Young, Susan M; Carter, Mark B; Bologa, Cristian G; Oprea, Tudor I; Satterthwait, Arnold C; Reed, John C; Edwards, Bruce S; Sklar, Larry A

    2011-10-01

    The human Bcl-2 family includes six antiapoptotic members (Bcl-2, Bcl-B, Bcl-W, Bcl-X(L), Bfl-1, and Mcl-1) and many proapoptotic members, wherein a balance between the two determines cell life or death in many physiological and disease contexts. Elevated expression of various antiapoptotic Bcl-2 members is commonly observed in cancers, and chemical inhibitors of these proteins have been shown to promote apoptosis of malignant cells in culture, in animal models, and in human clinical trials. All six antiapoptotic members bind a helix from the proapoptotic family member Bim, thus quenching Bim's apoptotic signal. Here, we describe the use of a multiplex, high-throughput flow cytometry assay for the discovery of small molecule modulators that disrupt the interaction between the antiapoptotic members of the Bcl-2 family and Bim. The six antiapoptotic Bcl-2 family members were expressed as glutathione-S-transferase fusion proteins and bound individually to six glutathione bead sets, with each set having a different intensity of red fluorescence. A fluorescein-conjugated Bcl-2 homology region 3 (BH3) peptide from Bim was employed as a universal ligand. Flow cytometry measured the amount of green peptide bound to each bead set in a given well, with inhibitory compounds resulting in a decrease of green fluorescence on one or more bead set(s). Hits and cheminformatically selected analogs were retested in a dose-response series, resulting in three "active" compounds for Bcl-B. These three compounds were validated by fluorescence polarization and isothermal titration calorimetry. We discuss some of the lessons learned about screening a chemical library provided by the National Institutes of Health Small Molecule Repository (∼195,000 compounds) using high-throughput flow cytometry.

  13. Extended multiplexing of TMT labeling reveals age and high fat diet specific proteome changes in mouse epididymal adipose tissue.

    Science.gov (United States)

    Plubell, Deanna L; Wilmarth, Phillip A; Zhao, Yuqi; Fenton, Alexandra M; Minnier, Jessica; Reddy, Ashok P; Klimek, John; Yang, Xia; David, Larry L; Pamir, Nathalie

    2017-03-21

    The lack of high-throughput methods to analyze the adipose tissue protein composition limits our understanding of the protein networks responsible for age and diet related metabolic response. We have developed an approach using multiple-dimension liquid chromatography tandem mass spectrometry and extended multiplexing (24 biological samples) with TMT labeling to analyze proteomes of epididymal adipose tissues isolated from mice fed either low or high fat diet for a short or a long-term, and from mice that aged on low vs. high fat diets. The peripheral metabolic health (as measured by body weight, adiposity, plasma fasting glucose, insulin, triglycerides, total cholesterol levels, and glucose and insulin tolerance tests) deteriorated with diet and advancing age, with long-term high fat diet exposure being the worst. In response to short-term high fat diet, 43 proteins representing lipid metabolism (e.g., AACS, ACOX1, ACLY) and red-ox pathways (e.g., CPD2, CYP2E, SOD3) were significantly altered (FDR fat diet significantly altered 55 proteins associated with immune response (e.g., IGTB2, IFIT3, LGALS1) and rennin angiotensin system (e.g. ENPEP, CMA1, CPA3, ANPEP). Age-related changes on low fat diet significantly altered only 18 proteins representing mainly urea cycle (e.g., OTC, ARG1, CPS1), and amino acid biosynthesis (e.g., GMT, AKR1C6). Surprisingly, high fat diet driven age-related changes culminated with alterations in 155 proteins involving primarily the urea cycle (e.g., ARG1, CPS1), immune response/complement activation (e.g., C3, C4b, C8, C9, CFB, CFH, FGA), extracellular remodeling (e.g., EFEMP1, FBN1, FBN2, LTBP4, FERMT2, ECM1, EMILIN2, ITIH3) and apoptosis (e.g., YAP1, HIP1, NDRG1, PRKCD, MUL1) pathways. Using our adipose tissue tailored approach we have identified both age-related and high fat diet specific proteomic signatures highlighting a pronounced involvement of arginine metabolism in response to advancing age, and branched chain amino acid

  14. Conformable actively multiplexed high-density surface electrode array for brain interfacing

    Science.gov (United States)

    Rogers, John; Kim, Dae-Hyeong; Litt, Brian; Viventi, Jonathan

    2015-01-13

    Provided are methods and devices for interfacing with brain tissue, specifically for monitoring and/or actuation of spatio-temporal electrical waveforms. The device is conformable having a high electrode density and high spatial and temporal resolution. A conformable substrate supports a conformable electronic circuit and a barrier layer. Electrodes are positioned to provide electrical contact with a brain tissue. A controller monitors or actuates the electrodes, thereby interfacing with the brain tissue. In an aspect, methods are provided to monitor or actuate spatio-temporal electrical waveform over large brain surface areas by any of the devices disclosed herein.

  15. VCSELs with a high-index-contrast grating for mode-division multiplexing

    DEFF Research Database (Denmark)

    Ran, Qijiang; Mørk, Jesper

    2013-01-01

    to excite a specific transverse mode, while its transmission phase is kept spatially constant. This laser can provide the selective excitation of a specific transverse mode, leading to a high coupling efficiency to a few mode fiber. Compared to the phase plate approach in current SDM systems, the HCG...

  16. Growth and multiplexed analysis of microorganisms on a subdivided, highly porous, inorganic chip manufactured from anopore

    NARCIS (Netherlands)

    Ingham, C.J.; Ende, van den M.; Pijnenburg, D.; Wever, P.C.; Schneeberger, P.M.

    2005-01-01

    A highly porous inorganic material (Anopore) was shown to be an effective support for culturing and imaging a wide range of microorganisms. An inert barrier grid was printed on the rigid surface of Anopore to create a "living chip" of 336 miniaturized compartments (200/cm2) with broad applications i

  17. Search for Diffuse Astrophysical Neutrino Flux Using Ultra-High Energy Upward-Going Muons in Super-Kamiokande I

    CERN Document Server

    Swanson, M E C; Hosaka, J; Iida, T; Ishihara, K; Kameda, J; Koshio, Y; Minamino, A; Mitsuda, C; Miura, M; Moriyama, S; Nakahata, M; Obayashi, Y; Ogawa, H; Shiozawa, M; Suzuki, Y; Takeda, A; Takeuchi, Y; Ueshima, K; Higuchi, I; Ishihara, C; Ishitsuka, M; Kajita, T; Kaneyuki, K; Mitsuka, G; Nakayama, S; Nishino, H; Okada, A; Okumura, K; Saji, C; Takenaga, Y; Clark, S; Desai, S; Dufour, F; Kearns, E; Likhoded, S; Litos, M; Raaf, Jennifer L; Stone, J L; Sulak, L R; Wang, W; Goldhaber, M; Casper, D; Cravens, J P; Dunmore, J; Kropp, W R; Liu, D W; Mine, S; Regis, C; Smy, M B; Sobel, H W; Vagins, M R; Ganezer, K S; Hill, J E; Keig, W E; Jang, J S; Kim, J Y; Lim, I T; Scholberg, K; Tanimoto, N; Walter, C W; Wendell, R; Ellsworth, R W; Tasaka, S; Guillian, G; Learned, J G; Matsuno, S; Messier, M D; Hayato, Y; Ichikawa, A K; Ishida, T; Ishii, T; Iwashita, T; Kobayashi, T; Nakadaira, T; Nakamura, K; Nitta, K; Oyama, Y; Totsuka, Y; Suzuki, A T; Hasegawa, M; Hiraide, K; Kato, I; Maesaka, H; Nakaya, T; Nishikawa, K; Sasaki, T; Sato, H; Yamamoto, S; Yokoyama, M; Haines, T J; Dazeley, S; Hatakeyama, S; Svoboda, R; Sullivan, G W; Turcan, D; Cooley, J; Mahn, K B M; Habig, A; Fukuda, Y; Sato, T; Itow, Y; Koike, T; Jung, C K; Kato, T; Kobayashi, K; Malek, M; McGrew, C; Sarrat, A; Terri, R; Yanagisawa, C; Tamura, N; Sakuda, M; Sugihara, M; Kuno, Y; Yoshida, M; Kim, S B; Yang, B S; Yoo, J; Ishizuka, T; Okazawa, H; Choi, Y; Seo, H K; Gando, Y; Hasegawa, T; Inoue, K; Ishii, H; Nishijima, K; Ishino, H; Watanabe, Y; Koshiba, M; Kielczewska, D; Zalipska, J; Berns, H G; Gran, R; Shiraishi, K K; Stachyra, A; Thrane, E; Washburn, K; Wilkes, R J002626770 100 L; Adams, J

    2006-01-01

    Many astrophysical models predict a diffuse flux of high energy neutrinos from active galactic nuclei and other extra-galactic sources. At muon energies above a TeV, the upward-going muon flux induced by neutrinos from active galactic nuclei is expected to exceed the flux due to atmospheric neutrinos. We have performed a search for this astrophysical neutrino flux by looking for upward-going muons in the highest energy data sample from the Super-Kamiokande detector using 1679.6 live days of data. We found 1 extremely high energy upward-going muon event, compared with an expected atmospheric neutrino background of 0.46 plus or minus 0.23 events. Using this result, we set an upper limit on the diffuse flux of upward-going muons due to neutrinos from astrophysical sources in the muon energy range 3.16-100 TeV.

  18. Microfluidic train station: highly robust and multiplexable sorting of droplets on electric rails.

    Science.gov (United States)

    Frenzel, Daniel; Merten, Christoph A

    2017-02-24

    Fluorescence-activated droplet sorting (FADS) has become a widely used technique for high-throughput screening applications. However, existing methods are very sensitive to fluctuating flow rates at the sorting junction, which can be caused by the pulsing effects of mechanical pumps, droplet aggregates or the accumulation of precipitates during lengthy biological screening applications. Furthermore, existing sorting devices allow only 2-way sorting. We present here a dielectrophoretic sorting system in which the droplets are sorted along multiple electrode pairs that run parallel to the channels. This enables highly reliable sorting (no errors were detected for more than 2000 sorting events) even when inverting the relative flow rates at a 2-way sorting junction from 80 : 20 to 20 : 80. Furthermore, our toolbox is scalable: we demonstrate on the example of a triple-colour sorting experiment with a total of four decoupled electrodes that multi-way sorting is feasible.

  19. Silicon on-chip bandpass filters for the multiplexing of high sensitivity photonic crystal microcavity biosensors

    Energy Technology Data Exchange (ETDEWEB)

    Yan, Hai, E-mail: hai.yan@utexas.edu; Zou, Yi; Yang, Chun-Ju [Department of Electrical and Computer Engineering, Microelectronics Research Center, The University of Texas at Austin, 10100 Burnet Rd., Austin, Texas 78758 (United States); Chakravarty, Swapnajit, E-mail: swapnajit.chakravarty@omegaoptics.com [Omega Optics, Inc., 8500 Shoal Creek Blvd., Austin, Texas 78757 (United States); Wang, Zheng [Department of Electrical and Computer Engineering, Microelectronics Research Center, The University of Texas at Austin, 10100 Burnet Rd., Austin, Texas 78758 (United States); Materials Science and Engineering Program, Texas Materials Institute, The University of Texas at Austin, Austin, Texas 78712 (United States); Tang, Naimei; Chen, Ray T., E-mail: raychen@uts.cc.utexas.edu [Department of Electrical and Computer Engineering, Microelectronics Research Center, The University of Texas at Austin, 10100 Burnet Rd., Austin, Texas 78758 (United States); Omega Optics, Inc., 8500 Shoal Creek Blvd., Austin, Texas 78757 (United States); Fan, Donglei [Materials Science and Engineering Program, Texas Materials Institute, The University of Texas at Austin, Austin, Texas 78712 (United States); Department of Mechanical Engineering, The University of Texas at Austin, Austin, Texas 78712 (United States)

    2015-03-23

    A method for the dense integration of high sensitivity photonic crystal (PC) waveguide based biosensors is proposed and experimentally demonstrated on a silicon platform. By connecting an additional PC waveguide filter to a PC microcavity sensor in series, a transmission passband is created, containing the resonances of the PC microcavity for sensing purpose. With proper engineering of the passband, multiple high sensitivity PC microcavity sensors can be integrated into microarrays and be interrogated simultaneously between a single input and a single output port. The concept was demonstrated with a 2-channel L55 PC biosensor array containing PC waveguide filters. The experiment showed that the sensors on both channels can be monitored simultaneously from a single output spectrum. Less than 3 dB extra loss for the additional PC waveguide filter is observed.

  20. DNA nanostructure-based universal microarray platform for high-efficiency multiplex bioanalysis in biofluids.

    Science.gov (United States)

    Li, Zhenhua; Zhao, Bin; Wang, Dongfang; Wen, Yanli; Liu, Gang; Dong, Haoqing; Song, Shiping; Fan, Chunhai

    2014-10-22

    Microarrays of biomolecules have greatly promoted the development of the fields of genomics, proteomics, and clinical assays because of their remarkably parallel and high-throughput assay capability. Immobilization strategies for biomolecules on a solid support surface play a crucial role in the fabrication of high-performance biological microarrays. In this study, rationally designed DNA tetrahedra carrying three amino groups and one single-stranded DNA extension were synthesized by the self-assembly of four oligonucleotides, followed by high-performance liquid chromatography purification. We fabricated DNA tetrahedron-based microarrays by covalently coupling the DNA tetrahedron onto glass substrates. After their biorecognition capability was evaluated, DNA tetrahedron microarrays were utilized for the analysis of different types of bioactive molecules. The gap hybridization strategy, the sandwich configuration, and the engineering aptamer strategy were employed for the assay of miRNA biomarkers, protein cancer biomarkers, and small molecules, respectively. The arrays showed good capability to anchor capture biomolecules for improving biorecognition. Addressable and high-throughput analysis with improved sensitivity and specificity had been achieved. The limit of detection for let-7a miRNA, prostate specific antigen, and cocaine were 10 fM, 40 pg/mL, and 100 nM, respectively. More importantly, we demonstrated that the microarray platform worked well with clinical serum samples and showed good relativity with conventional chemical luminescent immunoassay. We have developed a novel approach for the fabrication of DNA tetrahedron-based microarrays and a universal DNA tetrahedron-based microarray platform for the detection of different types of bioactive molecules. The microarray platform shows great potential for clinical diagnosis.

  1. Super Factories

    Indian Academy of Sciences (India)

    D G Hitlin

    2006-11-01

    Heavy-flavor physics, in particular and physics results from the factories, currently provides strong constraints on models of physics beyond the Standard Model. A new generation of colliders, Super Factories, with 50 to 100 times the luminosity of existing colliders, can, in a dialog with LHC and ILC, provide unique clarification of new physics phenomena seen at those machines.

  2. A Rapid, Multiplexed, High-Throughput Flow-Through Membrane Immunoassay: A Convenient Alternative to ELISA

    Directory of Open Access Journals (Sweden)

    Gonzalo J. Domingo

    2013-04-01

    Full Text Available This paper describes a rapid, high-throughput flow-through membrane immunoassay (FMIA platform. A nitrocellulose membrane was spotted in an array format with multiple capture and control reagents for each sample detection area, and assay steps were carried out by sequential aspiration of sample and reagents through each detection area using a 96-well vacuum manifold. The FMIA provides an alternate assay format with several advantages over ELISA. The high surface area of the membrane permits high label concentration using gold labels, and the small pores and vacuum control provide rapid diffusion to reduce total assay time to ~30 min. All reagents used in the FMIA are compatible with dry storage without refrigeration. The results appear as colored spots on the membrane that can be quantified using a flatbed scanner. We demonstrate the platform for detection of IgM specific to lipopolysaccharides (LPS derived from Salmonella Typhi. The FMIA format provides analytical results comparable to ELISA in less time, provides integrated assay controls, and allows compensation for specimen-to-specimen variability in background, which is a particular challenge for IgM assays.

  3. Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics.

    Science.gov (United States)

    Dixit, Chandra K; Kadimisetty, Karteek; Otieno, Brunah A; Tang, Chi; Malla, Spundana; Krause, Colleen E; Rusling, James F

    2016-01-21

    Early detection and reliable diagnostics are keys to effectively design cancer therapies with better prognoses. The simultaneous detection of panels of biomarker proteins holds great promise as a general tool for reliable cancer diagnostics. A major challenge in designing such a panel is to decide upon a coherent group of biomarkers which have higher specificity for a given type of cancer. The second big challenge is to develop test devices to measure these biomarkers quantitatively with high sensitivity and specificity, such that there are no interferences from the complex serum or tissue matrices. Lastly, integrating all these tests into a technology that does not require exclusive training to operate, and can be used at point-of-care (POC) is another potential bottleneck in futuristic cancer diagnostics. In this article, we review electrochemistry-based tools and technologies developed and/or used in our laboratories to construct low-cost microfluidic protein arrays for the highly sensitive detection of a panel of cancer-specific biomarkers with high specificity which at the same time has the potential to be translated into POC applications.

  4. Dedicated Searches for Low and High Mass Wimps with the SuperCDMS Soudan iZIP Detectors

    Energy Technology Data Exchange (ETDEWEB)

    Welliver, Bradford [Florida State U.

    2016-01-01

    Recent cosmological evidence suggests most of the mass of the universe takes the form of a type of particle that we have not been able to directly detect. Nearly 80 years that have elapsed since the rst hints of this dark matter started to appear from astronomers without any direct detection. The high precision era of cosmology and unifying models of particle physics developed in the 20 th century have presented us with an exciting mystery at the intersection of these two elds that needs to be solved. SuperCDMS Soudan operates specialized germanium detectors (iZIPs) that are cooled to milliKelvin temperatures deep underground in the Soudan Underground Laboratory with the hope of detecting a rare collision between dark matter and a nucleus. A search for low-mass dark matter comes with multiple unique challenges since the background discrimination abilities of these detectors becomes less powerful at the low energies needed to probe low-mass dark matter since the signal to noise ratio deteriorates. Using a sophisticated background model via a pulse rescaling technique, SuperCDMS Soudan was able to produce a world leading exclusion limit on low-mass dark matter. Effort is to extend the analysis to higher masses require long running times during which many aspects of the detectors or the environment can change. Additional challenges are offered by the powerful background discrimination ability of the iZIP. The background distributions are well separated from the signal region, meaning most of the leakage arises from low-probability tails of the background distributions. In the absence of an enormous dataset, extrapolations from the bulk of the distribution are required. While attempting to obtain a model of gamma induced electron-recoils leaking into the signal region of the detector from high radius a curious asymmetry between the sides of the detectors was discovered potentially indicating an electronics or detector design problem. This thesis describes the physics

  5. High resolution imaging in cross-section of a metal-oxide-semiconductor field-effect-transistor using super-higher-order nonlinear dielectric microscopy

    Science.gov (United States)

    Chinone, N.; Yamasue, K.; Honda, K.; Cho, Y.

    2013-11-01

    Scanning nonlinear dielectric microscopy (SNDM) can evaluate carrier or charge distribution in semiconductor devices. High sensitivity to capacitance variation enables SNDM to measure the super-high-order (higher than 3rd) derivative of local capacitance-voltage (C-V) characteristics directly under the tip (dnC/dVn,n = 3, 4, ...). We demonstrate improvement of carrier density resolution by measurement of dnC/dVn,n = 1, 2, 3, 4 (super-higher-order method) in the cross-sectional observation of metal-oxide-semiconductor field-effect-transistor.

  6. Multiplex PCR with minisequencing as an effective high-throughput SNP typing method for formalin-fixed tissue

    DEFF Research Database (Denmark)

    Gilbert, Marcus T P; Sanchez, Juan J; Haselkorn, Tamara;

    2007-01-01

    Extensive collections of formalin-fixed paraffin-embedded (FFPE) tissues exist that could be exploited for genetic analyses in order to provide important insights into the genetic basis of disease or host/pathogen cointeractions. We report here an evaluation of a 44 SNP multiplex genotyping metho...

  7. A high throughput single nucleotide polymorphism multiplex assay for parentage assignment in New Zealand sheep.

    Directory of Open Access Journals (Sweden)

    Shannon M Clarke

    Full Text Available Accurate pedigree information is critical to animal breeding systems to ensure the highest rate of genetic gain and management of inbreeding. The abundance of available genomic data, together with development of high throughput genotyping platforms, means that single nucleotide polymorphisms (SNPs are now the DNA marker of choice for genomic selection studies. Furthermore the superior qualities of SNPs compared to microsatellite markers allows for standardization between laboratories; a property that is crucial for developing an international set of markers for traceability studies. The objective of this study was to develop a high throughput SNP assay for use in the New Zealand sheep industry that gives accurate pedigree assignment and will allow a reduction in breeder input over lambing. This required two phases of development--firstly, a method of extracting quality DNA from ear-punch tissue performed in a high throughput cost efficient manner and secondly a SNP assay that has the ability to assign paternity to progeny resulting from mob mating. A likelihood based approach to infer paternity was used where sires with the highest LOD score (log of the ratio of the likelihood given parentage to likelihood given non-parentage are assigned. An 84 "parentage SNP panel" was developed that assigned, on average, 99% of progeny to a sire in a problem where there were 3,000 progeny from 120 mob mated sires that included numerous half sib sires. In only 6% of those cases was there another sire with at least a 0.02 probability of paternity. Furthermore dam information (either recorded, or by genotyping possible dams was absent, highlighting the SNP test's suitability for paternity testing. Utilization of this parentage SNP assay will allow implementation of progeny testing into large commercial farms where the improved accuracy of sire assignment and genetic evaluations will increase genetic gain in the sheep industry.

  8. High-Q wavelength division multiplexed optoelectronic oscillator based on a cascaded multi-loop topology

    Science.gov (United States)

    Charalambous, Georgios; Hasanuzzaman, G. K. M.; Perentos, Andreas; Iezekiel, Stavros

    2017-03-01

    A WDM optoelectronic oscillator (OEO) based on a cascaded optical multi-loop configuration and multiple photodiodes is proposed and demonstrated experimentally. By employing up to three lasers widely separated in wavelength along with two cascaded multi-loop fiber sections and two photodiodes, we demonstrate OEO topologies that scale up to six effective loops revealing an ultra-high quality factor in excess of 1010 and a phase noise performance down to -119 dBc/Hz at 10 kHz offset

  9. A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.

    Science.gov (United States)

    Ma, Xingliang; Zhang, Qunyu; Zhu, Qinlong; Liu, Wei; Chen, Yan; Qiu, Rong; Wang, Bin; Yang, Zhongfang; Li, Heying; Lin, Yuru; Xie, Yongyao; Shen, Rongxin; Chen, Shuifu; Wang, Zhi; Chen, Yuanling; Guo, Jingxin; Chen, Letian; Zhao, Xiucai; Dong, Zhicheng; Liu, Yao-Guang

    2015-08-01

    CRISPR/Cas9 genome targeting systems have been applied to a variety of species. However, most CRISPR/Cas9 systems reported for plants can only modify one or a few target sites. Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants. We designed PCR-based procedures to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning by Golden Gate ligation or Gibson Assembly. With this system, we edited 46 target sites in rice with an average 85.4% rate of mutation, mostly in biallelic and homozygous status. We reasoned that about 16% of the homozygous mutations in rice were generated through the non-homologous end-joining mechanism followed by homologous recombination-based repair. We also obtained uniform biallelic, heterozygous, homozygous, and chimeric mutations in Arabidopsis T1 plants. The targeted mutations in both rice and Arabidopsis were heritable. We provide examples of loss-of-function gene mutations in T0 rice and T1 Arabidopsis plants by simultaneous targeting of multiple (up to eight) members of a gene family, multiple genes in a biosynthetic pathway, or multiple sites in a single gene. This system has provided a versatile toolbox for studying functions of multiple genes and gene families in plants for basic research and genetic improvement. Copyright © 2015 The Author. Published by Elsevier Inc. All rights reserved.

  10. Dispersion-optimized optical fiber for high-speed long-haul dense wavelength division multiplexing transmission

    Science.gov (United States)

    Wu, Jindong; Chen, Liuhua; Li, Qingguo; Wu, Wenwen; Sun, Keyuan; Wu, Xingkun

    2011-07-01

    Four non-zero-dispersion-shifted fibers with almost the same large effective area (Aeff) and optimized dispersion properties are realized by novel index profile designing and modified vapor axial deposition and modified chemical vapor deposition processes. An Aeff of greater than 71 μm2 is obtained for the designed fibers. Three of the developed fibers with positive dispersion are improved by reducing the 1550nm dispersion slope from 0.072ps/nm2/km to 0.063ps/nm2/km or 0.05ps/nm2/km, increasing the 1550nm dispersion from 4.972ps/nm/km to 5.679ps/nm/km or 7.776ps/nm/km, and shifting the zero-dispersion wavelength from 1500nm to 1450nm. One of these fibers is in good agreement with G655D and G.656 fibers simultaneously, and another one with G655E and G.656 fibers; both fibers are beneficial to high-bit long-haul dense wavelength division multiplexing systems over S-, C-, and L-bands. The fourth developed fiber with negative dispersion is also improved by reducing the 1550nm dispersion slope from 0.12ps/nm2/km to 0.085ps/nm2/km, increasing the 1550nm dispersion from -4ps/nm/km to -6.016ps/nm/km, providing facilities for a submarine transmission system. Experimental measurements indicate that the developed fibers all have excellent optical transmission and good macrobending and splice performances.

  11. A multiplexed high-resolution imaging spectrometer for resonant inelastic soft X-ray scattering spectroscopy.

    Science.gov (United States)

    Warwick, Tony; Chuang, Yi De; Voronov, Dmitriy L; Padmore, Howard A

    2014-07-01

    The optical design of a two-dimensional imaging soft X-ray spectrometer is described. A monochromator will produce a dispersed spectrum in a narrow vertical illuminated stripe (∼2 µm wide by ∼2 mm tall) on a sample. The spectrometer will use inelastically scattered X-rays to image the extended field on the sample in the incident photon energy direction (vertical), resolving the incident photon energy. At the same time it will image and disperse the scattered photons in the orthogonal (horizontal) direction, resolving the scattered photon energy. The principal challenge is to design a system that images from the flat-field illumination of the sample to the flat field of the detector and to achieve sufficiently high spectral resolution. This spectrometer provides a completely parallel resonant inelastic X-ray scattering measurement at high spectral resolution (∼30,000) over the energy bandwidth (∼5 eV) of a soft X-ray absorption resonance.

  12. Multiplexed high-content analysis of mitochondrial morphofunction using live-cell microscopy.

    Science.gov (United States)

    Iannetti, Eligio F; Smeitink, Jan A M; Beyrath, Julien; Willems, Peter H G M; Koopman, Werner J H

    2016-09-01

    Mitochondria have a central role in cellular (patho)physiology, and they display a highly variable morphology that is probably coupled to their functional state. Here we present a protocol that allows unbiased and automated quantification of mitochondrial 'morphofunction' (i.e., morphology and membrane potential), cellular parameters (size, confluence) and nuclear parameters (number, morphology) in intact living primary human skin fibroblasts (PHSFs). Cells are cultured in 96-well plates and stained with tetramethyl rhodamine methyl ester (TMRM), calcein-AM (acetoxy-methyl ester) and Hoechst 33258. Next, multispectral fluorescence images are acquired using automated microscopy and processed to extract 44 descriptors. Subsequently, the descriptor data are subjected to a quality control (QC) algorithm based upon principal component analysis (PCA) and interpreted using univariate, bivariate and multivariate analysis. The protocol requires a time investment of ∼4 h distributed over 2 d. Although it is specifically developed for PHSFs, which are widely used in preclinical research, the protocol is portable to other cell types and can be scaled up for implementation in high-content screening.

  13. High-throughput Multiplexed xMAP Luminex Array Panel for Detection of Twenty TWO Medically Important Mosquito-borne Arboviruses based on Innovations in Synthetic Biology

    Science.gov (United States)

    Glushakova, Lyudmyla G.; Bradley, Andrea; Bradley, Kevin M.; Alto, Barry W.; Hoshika, Shuichi; Hutter, Daniel; Sharma, Nidhi; Yang, Zunyi; Kim, Myong-Jung; Benner, Steven A.

    2015-01-01

    Mosquito-borne arboviruses are emerging world-wide as important human and animal pathogens. This makes assays for their accurate and rapid identification essential for public health, epidemiological, ecological studies. Over the past decade, many mono- and multiplexed assays targeting arboviruses nucleic acids have been reported. None has become established for the routine identification of multiple viruses in a “single tube” setting. With increasing multiplexing, the detection of viral RNAs is complicated by noise, false positives and negatives. In this study, an assay was developed that avoids these problems by combining two new kinds of nucleic acids emerging from the field of synthetic biology. The first is a “self-avoiding molecular recognition system” (SAMRS), which enables high levels of multiplexing. The second is an “artificially expanded genetic information system” (AEGIS), which enables clean PCR amplification in nested PCR formats. A conversion technology was used to place AEGIS component into amplicon, improving their efficiency of hybridization on Luminex beads. When Luminex “liquid microarrays” are exploited for downstream detection, this combination supports single-tube PCR amplification assays that can identify 22 mosquito-borne RNA viruses from the genera Flavivirus, Alphavirus, Orthobunyavirus. The assay differentiates between closely-related viruses, as dengue, West Nile, Japanese encephalitis, and the California serological group. The performance and the sensitivity of the assay were evaluated with dengue viruses and infected mosquitoes; as few as 6–10 dengue virions can be detected in a single mosquito. PMID:25680538

  14. Highly multiplex and sensitive SNP genotyping method using a three-color fluorescence-labeled ligase detection reaction coupled with conformation-sensitive CE.

    Science.gov (United States)

    Choi, Woong; Jung, Gyoo Yeol

    2017-02-01

    For the development of clinically useful genotyping methods for SNPs, accuracy, simplicity, sensitivity, and cost-effectiveness are the most important criteria. Among the methods currently being developed for SNP genotyping technology, the ligation-dependent method is considered the simplest for clinical diagnosis. However, sensitivity is not guaranteed by the ligation reaction alone, and analysis of multiple targets is limited by the detection method. Although CE is an attractive alternative to error-prone hybridization-based detection, the multiplex assay process is complicated because of the size-based DNA separation principle. In this study, we employed the ligase detection reaction coupled with high-resolution CE-SSCP to develop an accurate, sensitive, and simple multiplex genotyping method. Ligase detection reaction could amplify ligated products through recurrence of denaturation and ligation reaction, and SSCP could separate these products according to each different structure conformation without size variation. Thus, simple and sensitive SNP analysis can be performed using this method involving the use of similar-sized probes, without complex probe design steps. We found that this method could not only accurately discriminate base mismatches but also quantitatively detect 37 SNPs of the tp53 gene, which are used as targets in multiplex analysis, using three-color fluorescence-labeled probes.

  15. High-throughput multiplexed xMAP Luminex array panel for detection of twenty two medically important mosquito-borne arboviruses based on innovations in synthetic biology.

    Science.gov (United States)

    Glushakova, Lyudmyla G; Bradley, Andrea; Bradley, Kevin M; Alto, Barry W; Hoshika, Shuichi; Hutter, Daniel; Sharma, Nidhi; Yang, Zunyi; Kim, Myong-Jung; Benner, Steven A

    2015-03-01

    Mosquito-borne arboviruses are emerging world-wide as important human and animal pathogens. This makes assays for their accurate and rapid identification essential for public health, epidemiological, ecological studies. Over the past decade, many mono- and multiplexed assays targeting arboviruses nucleic acids have been reported. None has become established for the routine identification of multiple viruses in a "single tube" setting. With increasing multiplexing, the detection of viral RNAs is complicated by noise, false positives and negatives. In this study, an assay was developed that avoids these problems by combining two new kinds of nucleic acids emerging from the field of synthetic biology. The first is a "self-avoiding molecular recognition system" (SAMRS), which enables high levels of multiplexing. The second is an "artificially expanded genetic information system" (AEGIS), which enables clean PCR amplification in nested PCR formats. A conversion technology was used to place AEGIS component into amplicon, improving their efficiency of hybridization on Luminex beads. When Luminex "liquid microarrays" are exploited for downstream detection, this combination supports single-tube PCR amplification assays that can identify 22 mosquito-borne RNA viruses from the genera Flavivirus, Alphavirus, Orthobunyavirus. The assay differentiates between closely-related viruses, as dengue, West Nile, Japanese encephalitis, and the California serological group. The performance and the sensitivity of the assay were evaluated with dengue viruses and infected mosquitoes; as few as 6-10 dengue virions can be detected in a single mosquito. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Modeling high adsorption capacity and kinetics of organic macromolecules on super-powdered activated carbon.

    Science.gov (United States)

    Matsui, Yoshihiko; Ando, Naoya; Yoshida, Tomoaki; Kurotobi, Ryuji; Matsushita, Taku; Ohno, Koichi

    2011-02-01

    The capacity to adsorb natural organic matter (NOM) and polystyrene sulfonates (PSSs) on small particle-size activated carbon (super-powdered activated carbon, SPAC) is higher than that on larger particle-size activated carbon (powdered-activated carbon, PAC). Increased adsorption capacity is likely attributable to the larger external surface area because the NOM and PSS molecules do not completely penetrate the adsorbent particle; they preferentially adsorb near the outer surface of the particle. In this study, we propose a new isotherm equation, the Shell Adsorption Model (SAM), to explain the higher adsorption capacity on smaller adsorbent particles and to describe quantitatively adsorption isotherms of activated carbons of different particle sizes: PAC and SPAC. The SAM was verified with the experimental data of PSS adsorption kinetics as well as equilibrium. SAM successfully characterized PSS adsorption isotherm data for SPACs and PAC simultaneously with the same model parameters. When SAM was incorporated into an adsorption kinetic model, kinetic decay curves for PSSs adsorbing onto activated carbons of different particle sizes could be simultaneously described with a single kinetics parameter value. On the other hand, when SAM was not incorporated into such an adsorption kinetic model and instead isotherms were described by the Freundlich model, the kinetic decay curves were not well described. The success of the SAM further supports the adsorption mechanism of PSSs preferentially adsorbing near the outer surface of activated carbon particles.

  17. Multiplex target enrichment using DNA indexing for ultra-high throughput SNP detection.

    LENUS (Irish Health Repository)

    Kenny, Elaine M

    2011-02-01

    Screening large numbers of target regions in multiple DNA samples for sequence variation is an important application of next-generation sequencing but an efficient method to enrich the samples in parallel has yet to be reported. We describe an advanced method that combines DNA samples using indexes or barcodes prior to target enrichment to facilitate this type of experiment. Sequencing libraries for multiple individual DNA samples, each incorporating a unique 6-bp index, are combined in equal quantities, enriched using a single in-solution target enrichment assay and sequenced in a single reaction. Sequence reads are parsed based on the index, allowing sequence analysis of individual samples. We show that the use of indexed samples does not impact on the efficiency of the enrichment reaction. For three- and nine-indexed HapMap DNA samples, the method was found to be highly accurate for SNP identification. Even with sequence coverage as low as 8x, 99% of sequence SNP calls were concordant with known genotypes. Within a single experiment, this method can sequence the exonic regions of hundreds of genes in tens of samples for sequence and structural variation using as little as 1 μg of input DNA per sample.

  18. Effect of weld heat input on toughness and structure of HAZ of a new super-high strength steel

    Indian Academy of Sciences (India)

    Wang Juan; Li Yajiang; Liu Peng

    2003-04-01

    Fracture morphology and fine structure in the heat-affected zone (HAZ) of HQ130 super-high strength steel are studied by means of SEM, TEM and electron diffraction technique. Test results indicated that the structure of HAZ of HQ130 steel was mainly lath martensite (ML), in which there were a lot of dislocations in the sub-structure inside ML lath, the dislocation density was about (0.3 ∼ 0.9) × 1012/cm2. No obvious twin was observed in the HAZ under the condition of normal weld heat input. By controlling weld heat input ($E \\leq$ 20 kJ/cm), the impact toughness in the HAZ can be assured.

  19. Proteomic Analysis of the Response of Liangyoupeijiu (Super High-Yield Hybrid Rice) Seedlings to Cold Stress

    Institute of Scientific and Technical Information of China (English)

    Ping-Fang Yang; Xiao-Juan Li; Yu Liang; Yu-Xiang Jing; Shi-Hua Shen; Ting-Yun Kuang

    2006-01-01

    Liangyoupeijiu is a super high-yield hybrid rice. Despite its advantages with respect to yield and grain quality, it is sensitive to cold, which keeps it from being widely cultivated. We subjected Liangyoupeijiu seedlings to 4 ℃ cold treatment, then extracted the leaf proteins. After 2-D gel electrophoresis separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, a series of differentially displayed proteins were identified. Some metabolism-associated proteins were found among the downregulated proteins, such as carbamoyl phosphate synthetase, transketolase 1, dihydrolipoamide dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase. The upregulated proteins included both stress-resistance proteins such as nucleoside diphosphate kinase Ⅰ and proteins that are negative for rice growth, such as FtsH-like protein, plastid fusion and/or translocation factor (Pftf) and actin. Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolic activity and damaging cell structure.

  20. A Low Collision and High Throughput Data Collection Mechanism for Large-Scale Super Dense Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Chunyang Lei

    2016-07-01

    Full Text Available Super dense wireless sensor networks (WSNs have become popular with the development of Internet of Things (IoT, Machine-to-Machine (M2M communications and Vehicular-to-Vehicular (V2V networks. While highly-dense wireless networks provide efficient and sustainable solutions to collect precise environmental information, a new channel access scheme is needed to solve the channel collision problem caused by the large number of competing nodes accessing the channel simultaneously. In this paper, we propose a space-time random access method based on a directional data transmission strategy, by which collisions in the wireless channel are significantly decreased and channel utility efficiency is greatly enhanced. Simulation results show that our proposed method can decrease the packet loss rate to less than 2 % in large scale WSNs and in comparison with other channel access schemes for WSNs, the average network throughput can be doubled.

  1. A numerical study of super-resolution through fast 3D wideband algorithm for scattering in highly-heterogeneous media

    KAUST Repository

    Létourneau, Pierre-David

    2016-09-19

    We present a wideband fast algorithm capable of accurately computing the full numerical solution of the problem of acoustic scattering of waves by multiple finite-sized bodies such as spherical scatterers in three dimensions. By full solution, we mean that no assumption (e.g. Rayleigh scattering, geometrical optics, weak scattering, Born single scattering, etc.) is necessary regarding the properties of the scatterers, their distribution or the background medium. The algorithm is also fast in the sense that it scales linearly with the number of unknowns. We use this algorithm to study the phenomenon of super-resolution in time-reversal refocusing in highly-scattering media recently observed experimentally (Lemoult et al., 2011), and provide numerical arguments towards the fact that such a phenomenon can be explained through a homogenization theory.

  2. A Low Collision and High Throughput Data Collection Mechanism for Large-Scale Super Dense Wireless Sensor Networks

    Science.gov (United States)

    Lei, Chunyang; Bie, Hongxia; Fang, Gengfa; Gaura, Elena; Brusey, James; Zhang, Xuekun; Dutkiewicz, Eryk

    2016-01-01

    Super dense wireless sensor networks (WSNs) have become popular with the development of Internet of Things (IoT), Machine-to-Machine (M2M) communications and Vehicular-to-Vehicular (V2V) networks. While highly-dense wireless networks provide efficient and sustainable solutions to collect precise environmental information, a new channel access scheme is needed to solve the channel collision problem caused by the large number of competing nodes accessing the channel simultaneously. In this paper, we propose a space-time random access method based on a directional data transmission strategy, by which collisions in the wireless channel are significantly decreased and channel utility efficiency is greatly enhanced. Simulation results show that our proposed method can decrease the packet loss rate to less than 2% in large scale WSNs and in comparison with other channel access schemes for WSNs, the average network throughput can be doubled. PMID:27438839

  3. A Low Collision and High Throughput Data Collection Mechanism for Large-Scale Super Dense Wireless Sensor Networks.

    Science.gov (United States)

    Lei, Chunyang; Bie, Hongxia; Fang, Gengfa; Gaura, Elena; Brusey, James; Zhang, Xuekun; Dutkiewicz, Eryk

    2016-07-18

    Super dense wireless sensor networks (WSNs) have become popular with the development of Internet of Things (IoT), Machine-to-Machine (M2M) communications and Vehicular-to-Vehicular (V2V) networks. While highly-dense wireless networks provide efficient and sustainable solutions to collect precise environmental information, a new channel access scheme is needed to solve the channel collision problem caused by the large number of competing nodes accessing the channel simultaneously. In this paper, we propose a space-time random access method based on a directional data transmission strategy, by which collisions in the wireless channel are significantly decreased and channel utility efficiency is greatly enhanced. Simulation results show that our proposed method can decrease the packet loss rate to less than 2 % in large scale WSNs and in comparison with other channel access schemes for WSNs, the average network throughput can be doubled.

  4. Rapid multiplex high resolution melting method to analyze inflammatory related SNPs in preterm birth

    Directory of Open Access Journals (Sweden)

    Pereyra Silvana

    2012-01-01

    Full Text Available Abstract Background Complex traits like cancer, diabetes, obesity or schizophrenia arise from an intricate interaction between genetic and environmental factors. Complex disorders often cluster in families without a clear-cut pattern of inheritance. Genomic wide association studies focus on the detection of tens or hundreds individual markers contributing to complex diseases. In order to test if a subset of single nucleotide polymorphisms (SNPs from candidate genes are associated to a condition of interest in a particular individual or group of people, new techniques are needed. High-resolution melting (HRM analysis is a new method in which polymerase chain reaction (PCR and mutations scanning are carried out simultaneously in a closed tube, making the procedure fast, inexpensive and easy. Preterm birth (PTB is considered a complex disease, where genetic and environmental factors interact to carry out the delivery of a newborn before 37 weeks of gestation. It is accepted that inflammation plays an important role in pregnancy and PTB. Methods Here, we used real time-PCR followed by HRM analysis to simultaneously identify several gene variations involved in inflammatory pathways on preterm labor. SNPs from TLR4, IL6, IL1 beta and IL12RB genes were analyzed in a case-control study. The results were confirmed either by sequencing or by PCR followed by restriction fragment length polymorphism. Results We were able to simultaneously recognize the variations of four genes with similar accuracy than other methods. In order to obtain non-overlapping melting temperatures, the key step in this strategy was primer design. Genotypic frequencies found for each SNP are in concordance with those previously described in similar populations. None of the studied SNPs were associated with PTB. Conclusions Several gene variations related to the same inflammatory pathway were screened through a new flexible, fast and non expensive method with the purpose of analyzing

  5. SuperQuant

    DEFF Research Database (Denmark)

    Gorshkov, Vladimir; Verano-Braga, Thiago; Kjeldsen, Frank

    2015-01-01

    SuperQuant is a quantitative proteomics data processing approach that uses complementary fragment ions to identify multiple co-isolated peptides in tandem mass spectra allowing for their quantification. This approach can be applied to any shotgun proteomics data set acquired with high mass accura...... of the same proteins were close to the values typical for other precursor ion-based quantification methods. The raw data is deposited to ProteomeXchange (PXD001907). The developed node is available for testing at https://github.com/caetera/SuperQuantNode....

  6. High-Speed Imaging of a Water Droplet Impacting a Super Cold Surface

    KAUST Repository

    Khaled, Narimane

    2016-08-01

    Frost formation is of a major research interest as it can affect many industrial processes. Frost appears as a thin deposit of ice crystals when the temperature of the surface is below the freezing point of the liquid. The objective of this research is to study icing with hope to propose new anti-icing and deicing methods. In the beginning of the research, cracking of the ice layer was observed when a deionized water droplet impacts a ?50 oC cooled sphere surface that is in contact with dry ice. To further investigate the cracks occurrence, multiple experiments were conducted. It was observed that the sphere surface temperature and droplet temperature (ranges from 10-80 oC) have no effect on the crack formation. On the other hand, it was observed that formation of a thin layer of frost on the sphere before the drop impact leads the lateral cracking of the ice. Thus, attempts to reproduce the cracks on clean super cold sphere surfaces were made using scratched and sandblasted spheres as well as superhydrophobized and polymer particle coated spheres. Furthermore, innovative methods were tried to initiate the cracks by placing epoxy glue bumps and ice-islands coatings on the surface of the spheres. All of these attempts to reproduce the crack formation without the presence of frost, failed. Nonetheless, the adding of isolated frost on the sphere surfaces always leads to the crack formation. Generally, frost forms on the small spheres faster than it does on the bigger ones. Additionally, the cold water droplet produces thicker water and ice layer compared to a hot water droplet; and the smaller the sphere the larger its water and ice layer thicknesses.

  7. Silicon on-chip side-coupled high-Q micro-cavities for the multiplexing of high sensitivity photonic crystal integrated sensors array

    Science.gov (United States)

    Yang, Daquan; Wang, Chunhong; Yuan, Wei; Wang, Bo; Yang, Yujie; Ji, Yuefeng

    2016-09-01

    A novel two-dimensional (2D) silicon (Si) photonic crystal (PC) α-H0-slot micro-cavity with high Q-factor and high sensitivity (S) is presented. Based on the proposed α-H0-Slot micro-cavities, an optimal design of photonic crystal integrated sensors array (PC-ISA) on monolithic silicon on insulator (SOI) is displayed. By using finite-difference time-domain (FDTD) method, the simulation results demonstrate that both large S of 200 nm/RIU (RIU=refractive index unit) and high Q-factor >104 at telecom wavelength range can be achieved simultaneously. And the sensor figure of merit (FOM)>7000 is featured, an order of magnitude improvement over previous 2D PC sensors array. In addition, for the proposed 2D PC-ISA device, each sensor unit is shown to independently shift its resonance wavelength in response to the changes in refractive index (RI) and does not perturb the others. Thus, it is potentially an ideal platform for realizing ultra-compact lab-on-a-chip applications with dense arrays of functionalized spots for multiplexed sensing, and also can be used as an opto-fluidic architecture for performing highly parallel detection of biochemical interactions in aqueous environments.

  8. A novel lab-on-chip platform with integrated solid phase PCR and Supercritical Angle Fluorescence (SAF) microlens array for highly sensitive and multiplexed pathogen detection

    DEFF Research Database (Denmark)

    Hung, Tran Quang; Chin, Wai Hoe; Sun, Yi

    2016-01-01

    Solid-phase PCR (SP-PCR) has become increasingly popular for molecular diagnosis and there have been a few attempts to incorporate SP-PCR into lab-on-a-chip (LOC) devices. However, their applicability for on-line diagnosis is hindered by the lack of sensitive and portable on-chip optical detectio......-PCR and SAF microlens array allows for on-chip highly sensitive and multiplexed pathogen detection with low-cost and compact optical components. The LOC platform would be widely used as a high-throughput biosensor to analyze food, clinical and environmental samples....

  9. An Efficient Statistical Multiplexing Method for H.264 VBR Video Sources for Improved Traffic Smoothing

    Directory of Open Access Journals (Sweden)

    T.Raghuveera

    2010-04-01

    Full Text Available Frame level H.264/MPEG encoded VBR video traffic is highly bursty in nature because of inherent coding techniques employed. Multiplexing traffic from many VBR sources results in smoothing of generated traffic from the multiplexer, and improves Statistical Multiplexing Gain (SMG. An efficient multiplexing methodology can greatly enhance resource utilization. Performance of multiplexer can be estimated by addressing the burstiness and statistical multiplexing gain. We present here a new multiplexing method named “ERA multiplexing”, which is quite simple, faster and efficient as opposed to any other known methods like, Frame-aligned multiplexing, Frame-lag based multiplexing and random multiplexing. Our experiments have proved that ERA method is much superior in terms of smoothing the traffic and achieving better statistical multiplexing gain. We have tested the technique with high quality frame size traces of Star Wars-IV encoded using H.264/SVC and H.264/AVC to justify our claims

  10. Laneway's Side Stability Mechanism and Support Technology in Super-high Roadway%巨高巷道巷帮稳定机理及支护技术

    Institute of Scientific and Technical Information of China (English)

    李积星; 许磊; 蒋红军

    2012-01-01

    以山西某矿5203轨道巷的巨高巷道为研究对象,采用对比分析方法,描述了巨高巷道巷帮水平位移和下沉特征及塑性区的分布特征。通过分析巷帮片帮力学模型、巷帮锚固墙的对深部围岩约束原理、巨高巷道金属支架腿部易产生压杆失稳等,最终采用高强锚杆、带、网、索支护方式加固巷帮并确定了合理支护参数。实践结果表明,巨高巷道巷帮稳定原理适用于巨高巷道巷帮支护。%Taking super-high roadway appeared in 5203 track roadway in a mine of Shanxi as research object.The horizontal displacement,subsidence features and plastic zone distribution characteristics of laneway's side in super-high roadway are described with comparison analysis method in this paper.Through analyzing mechanical model of rib spalling,constraint theory of anchor wall of laneway's side on deep surrounding rock and pressure bar destabilization of metallic support generated in steel timber leg of super-high roadway,finally,the support form of using high strength bolts,steel strip,anchor net and anchor cable to reinforce laneway's side is adopted,and the reasonable supporting parameters are determined.The result of engineering practice proves that the laneway's side stabilization theory of super-high roadway is suitable to the laneway's side support of super-high roadside.

  11. Technological development for super-high efficiency solar cells. Technological development for super-high efficiency singlecrystalline silicon solar cells (super-high efficiency singlecrystalline Si solar cells); Chokokoritsu taiyo denchi no gijutsu kaihatsu. Chokokoritsu tankessho silicon taiyo denchi no gijutsu kaihatsu (chokokoritsu tankessho silicon taiyo denchi cell no gijutsu kaihatsu)

    Energy Technology Data Exchange (ETDEWEB)

    Tatsuta, M. [New Energy and Industrial Technology Development Organization, Tokyo (Japan)

    1994-12-01

    This paper reports the study results on technological development of super-high efficiency singlecrystalline silicon solar cells in fiscal 1994. (1) On development of high-performance light receiving layer, the fine electrode for receiving surfaces was designed to reduce serial resistance, and the high-quality oxide passivation film was studied to reduce surface recombination velocity. (2) On development of forming technology of back heterojunction, the high-quality cell with B-doped fine crystalline Si film on its back was studied by heat treatment of the fine crystalline Si film, and the cell structure with high back reflectance of light was also studied. (3) On analysis for high-efficiency cells, the relation between the back recombination velocity at the interface between p-type substrate and back passivation film, and the internal collection efficiency as probe light was injected from the back, was calculated by numerical simulation. As a result, the cell back recombination velocity could be evaluated by measuring the spectral internal collection efficiency to back injection. 15 figs., 6 tabs.

  12. Matter Composition and Two Stage Evolution of a Liangshan Super High-Sulfur Coal Seam in Kaili, Eastern Guizhou

    Institute of Scientific and Technical Information of China (English)

    YI Tong-sheng; QIN Yong; ZHANG Jing; WU Yan-yan; LI Zhuang-fu

    2007-01-01

    Super-high sulfur coal resultes in serious coal-derived pollution but might have a particular genesis. Thus, a columnar section of an Early Permian Liangshan Formation coal seam. weight average sulfur content 5.80%, from Kaili, eastern Guizhou, was studied using the methods of coal petrology and geochemistry. The results show that the seam was apparently formed in seawater-effected peat bogs that developed in two distinct stages. During the first stage various layers were formed in a supratidal bog and have a composition characteristic of a bog with a gradually decreasing seawater effect, decreasing water dynamics, and an increasingly reductive environment. Layers in the upper seam formed during a second stage in an intertidal bog. These layers are very high in total and inorganic sulfur, the ratios of organic/inorganic sulfur and V/I drop, they are high in coal ash yield and have a high ash component index, considerable barkinite, oxidized and detrital macerals, have a porphyroclatic micro-structure and are rich in pyrite, all of which indicate the coal-forming environment had higher oxidation potential, strong and roiling water dynamics, and intermittent exposure to a sulfur rich environment.

  13. The Super Patalan Numbers

    OpenAIRE

    Richardson, Thomas M.

    2014-01-01

    We introduce the super Patalan numbers, a generalization of the super Catalan numbers in the sense of Gessel, and prove a number of properties analagous to those of the super Catalan numbers. The super Patalan numbers generalize the super Catalan numbers similarly to how the Patalan numbers generalize the Catalan numbers.

  14. Super-high-frequency shielding properties of excimer-laser-synthesized-single-wall-carbon-nanotubes/polyurethane nanocomposite films

    Science.gov (United States)

    Aïssa, B.; Laberge, L. L.; Habib, M. A.; Denidni, T. A.; Therriault, D.; El Khakani, M. A.

    2011-04-01

    Electromagnetic shielding attenuation (ESA) properties of carbon nanotubes/polymer nanocomposite films, in the super high frequency (SHF) X-band (7-12 GHz) domain are studied. The nanocomposite films consisted of thermoset polyurethane (PU) resin blended with single-walled carbon nanotubes (SWCNTs) mats, and deposited on fused quartz substrates. Two different approaches were used to achieve the nanocomposite films, namely (i) through the on-substrate "all-laser" growth approach of SWCNTs directly onto substrate, followed by their infiltration by the PU resin, and (ii) by appropriately dispersing the chemically-purified SWCNTs (in the soot form) into the PU matrix and their subsequent deposition onto quartz substrates by means of a solvent casting process. Characterizations of the ESA properties of the developed nanocomposite films show that they exhibit systematically a deep shielding band, centered at around 9.5 GHz, with an attenuation as high as |- 30| dB, recorded for SWCNT loads of 2.5 wt. % and above. A direct correlation is established between the electrical conductivity of the nanocomposite films and their electromagnetic shielding capacity. The SWCNTs/PU nanocomposites developed here are highly promising shielding materials as SHF notch filters, as their ESA capacity largely exceeds the target value of |- 20| dB generally requested for commercial applications.

  15. A theoretical prediction of super high-performance thermoelectric materials based on MoS2/WS2 hybrid nanoribbons.

    Science.gov (United States)

    Zhang, Zhongwei; Xie, Yuee; Peng, Qing; Chen, Yuanping

    2016-02-17

    Modern society is hungry for electrical power. To improve the efficiency of energy harvesting from heat, extensive efforts seek high-performance thermoelectric materials that possess large differences between electronic and thermal conductance. Here we report a super high-performance material of consisting of MoS2/WS2 hybrid nanoribbons discovered from a theoretical investigation using nonequilibrium Green's function methods combined with first-principles calculations and molecular dynamics simulations. The hybrid nanoribbons show higher efficiency of energy conversion than the MoS2 and WS2 nanoribbons due to the fact that the MoS2/WS2 interface reduces lattice thermal conductivity more than the electron transport. By tuning the number of the MoS2/WS2 interfaces, a figure of merit ZT as high as 5.5 is achieved at a temperature of 600 K. Our results imply that the MoS2/WS2 hybrid nanoribbons have promising applications in thermal energy harvesting.

  16. Multiplexity and multireciprocity in directed multiplexes

    CERN Document Server

    Gemmetto, Valerio; Picciolo, Francesco; Ruzzenenti, Franco; Garlaschelli, Diego

    2014-01-01

    In the last few years, the study of multi-layer complex networks has received significant attention. In this work, we provide new measures to analyse dependencies between directed links in different layers of multiplex networks. We show that this requires more than a straightforward extension of the corresponding multiplexity measures that have been developed for undirected multiplexes. In particular, one should take into account the effects of reciprocity, i.e. the tendency of pairs of vertices to establish mutual connections. It is well known that reciprocity is a crucial property of many directed single-layer networks, affecting several dynamical processes taking place on such systems. Here we extend this quantity to directed multiplexes and introduce the notion of multireciprocity, defined as the tendency of links in one layer to be reciprocated by links in a different layer. We introduce multireciprocity measures valid for both binary and weighted networks and then validate these novel quantities on the ...

  17. High Throughput In Situ DDA Analysis of Neuropeptides by Coupling Novel Multiplex Mass Spectrometric Imaging (MSI) with Gas-Phase Fractionation

    Science.gov (United States)

    OuYang, Chuanzi; Chen, Bingming; Li, Lingjun

    2015-12-01

    Matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging (MSI) is a powerful tool to map the spatial distribution of biomolecules on tissue sections. Recent developments of hybrid MS instruments allow combination of different types of data acquisition by various mass analyzers into a single MSI analysis, which reduces experimental time and sample consumptions. Here, using the well-characterized crustacean nervous system as a test-bed, we explore the utility of high resolution and accurate mass (HRAM) MALDI Orbitrap platform for enhanced in situ characterization of the neuropeptidome with improved chemical information. Specifically, we report on a multiplex-MSI method, which combines HRAM MSI with data dependent acquisition (DDA) tandem MS analysis in a single experiment. This method enables simultaneous mapping of neuropeptide distribution, sequence validation, and novel neuropeptide discovery in crustacean neuronal tissues. To enhance the dynamic range and efficiency of in situ DDA, we introduced a novel approach of fractionating full m/z range into several sub-mass ranges and embedding the setup using the multiplex-DDA-MSI scan events to generate pseudo fractionation before MS/MS scans. The division of entire m/z into multiple segments of m/z sub-ranges for MS interrogation greatly decreased the complexity of molecular species from tissue samples and the heterogeneity of the distribution and variation of intensities of m/z peaks. By carefully optimizing the experimental conditions such as the dynamic exclusion, the multiplex-DDA-MSI approach demonstrates better performance with broader precursor coverage, less biased MS/MS scans towards high abundance molecules, and improved quality of tandem mass spectra for low intensity molecular species.

  18. What's So Super about Superfoods?

    Science.gov (United States)

    ... with meals. The Skinny on Common Super Foods Salmon is a fatty fish that’s low in saturated ... soy nuts are high in polyunsaturated fat, fiber, vitamins and minerals but low in saturated fat. They ...

  19. A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine

    Directory of Open Access Journals (Sweden)

    Xu-long Wu

    2017-01-01

    Full Text Available Here we aimed to develop a capillary electrophoresis-based high-throughput multiplex polymerase chain reaction (PCR system for the simultaneous detection of nine pathogens in swine. Nine pairs of specific primers and a set of universal primers were designed; the multiplex PCR was established. The specificity and cross-reactivity of this assay were examined, and the detection limit was determined using serial 10-fold dilutions of plasmids containing the target sequences. The assay was further tested using 144 clinical samples. We found that the nine specific amplification peaks were observed, and the assay had a high degree of specificity, without nonspecific amplification. The simultaneous detection limit for the nine viruses reached 10000 copies μL−1 when all of the premixed viral targets were present. Seventy-seven of the clinical samples tested positive for at least one of the viruses; the principal viral infections in the clinical samples were porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus. This approach has much potential for further development of high-throughput detection tools for the diagnosis of diseases in animals.

  20. Sandpiles on multiplex networks

    CERN Document Server

    Lee, Kyu-Min; Kim, I -M

    2011-01-01

    We introduce the sandpile model on multiplex networks with more than one type of edges and investigate its scaling and dynamical behaviors. We find that the introduction of multiplexity does not alter the scaling behavior of avalanche dynamics; the system is critical with the asymptotic power-law avalanche size distribution with exponent $\\tau = 3/2$ on duplex random networks. Detailed cascade dynamics, however, is affected by the multiplex coupling. For example, higher-degree nodes such as hubs in scale-free networks fail more often in the multiplex dynamics than in the simplex network counterpart in which different types of edges are simply aggregated. Our results suggest that multiplex modeling would be necessary in order to gain better understanding of cascading failure phenomena of real-world multiplex complex systems, such as the global economic crisis.

  1. Weighted Multiplex Networks

    CERN Document Server

    Menichetti, Giulia; Panzarasa, Pietro; Mondragón, Raúl J; Bianconi, Ginestra

    2013-01-01

    One of the most important challenges in network science is to quantify the information encoded in complex network structures. Disentangling randomness from organizational principles is even more demanding when networks have a multiplex nature. Multiplex networks are multilayer systems of $N$ nodes that can be linked in multiple interacting and co-evolving layers. In these networks, relevant information might not be captured if the single layers were analyzed separately. Here we demonstrate that such partial analysis of layers fails to capture significant correlations between weights and topology of complex multiplex networks. To this end, we study two weighted multiplex co-authorship and citation networks involving the authors included in the American Physical Society. We show that in these networks weights are strongly correlated with multiplex structure, and provide empirical evidence in favor of the advantage of studying weighted measures of multiplex networks, such as multistrength and the inverse multipa...

  2. High Solids Consolidated Incinerator Facility (CIF) Wastes Stabilization with Ceramicrete and Super Cement

    Energy Technology Data Exchange (ETDEWEB)

    Walker, B.W.

    1999-09-14

    High Solids ash and scrubber solution waste streams were generated at the incinerator facility at SRS by burning radioactive diatomaceous filter rolls which contained small amounts of uranium, and listed solvents (F and U). This report details solidification activities using selected Mixed Waste Focus Area (MWFA) technologies with the High Solids waste streams.

  3. Multi-photon resonance enhanced super high-order harmonic generation

    Institute of Scientific and Technical Information of China (English)

    Lin Zheng-Zhe; Zhuang Jun; Ning Xi-Jing

    2010-01-01

    This paper proposes highly charged ions pumped by intense laser to produce very high order harmonics.Numerical simulations and full quantum theory of Ne9+ ions driven by laser pulses at 1064 nm in the power range of 109 W/cm2 ~ 1015 W/cm2 show that the emission spectrum corresponds to the electronic transitions from the excited states to the ground state,which is very different from the spectrum of general high-order harmonic generation.In such situation,harmonic order as high as 1000 can be obtained without producing lower order harmonics and the energy conversion efficiency is close to general high order harmonic generation of hydrogen atom in the same laser field.

  4. OPTIMOS-EVE optical design of a very efficient, high-multiplex, large spectral coverage, fiber-fed spectrograph at EELT

    Science.gov (United States)

    Spanò, P.; Tosh, I.; Chemla, F.

    2010-07-01

    OPTIMOS-EVE is a fiber-fed, high-multiplex, high-efficiency, large spectral coverage spectrograph for EELT covering visible and near-infrared simultaneously. More than 200 seeing-limited objects will be observed at the same time over the full 7 arcmin field of view of the telescope, feeding the spectrograph, asking for very large multiplexing at the spectrograph side. The spectrograph consists of two identical units. Each unit will have two optimized channels to observe both visible and near-infrared wavelengths at the same time, covering from 0.37 to 1.7 micron. To maximize the scientific return, a large simultaneous spectral coverage per exposure was required, up to 1/3 of the central wavelength. Moreover, different spectral resolution modes, spanning from 5'000 to 30'000, were defined to match very different sky targets. Many different optical solutions were generated during the initial study phase in order to select that one that will maximize performances within given constraints (mass, space, cost). Here we present the results of this study, with special attention to the baseline design. Efforts were done to keep size of the optical components well within present state-of-the-art technologies. For example, large glass blank sizes were limited to ~35 cm maximum diameter. VPH gratings were selected as dispersers, to improve efficiency, following their superblaze curve. This led to scanning gratings and cameras. Optical design will be described, together with expected performances.

  5. High-resolution WRF simulation of cloud properties over the super typhoon Haiyan: physics parameterizations and comparison against MODIS

    Science.gov (United States)

    Islam, Tanvir; Srivastava, Prashant K.; Dai, Qiang

    2016-11-01

    Numerical weather prediction (NWP) models can complement the satellite technology in simulating the cloud properties, especially in extreme storm events, when gathering new data becomes more than essential for accurate weather forecasting. In this study, we investigate the capability of the Weather Research and Forecasting (WRF) model to realistically simulate some important cloud properties in high-resolution grids, such as cloud phase (e.g., liquid or ice) and cloud water path. The sensitivity of different combinations of physics parameterizations to the simulated cloud fields is studied. The experiment is conducted on a super typhoon event by configuring the WRF model in two domains, with two-way nesting, allowing bidirectional information exchange between the parent and the nest. In order to do the assessment, the simulated cloud fields are compared against MODIS-derived cloud properties from one overpass scene. While the simulations have been able to capture the spatial distribution of cloud properties reasonably well, produced cloud quantities such as ice water path has been significantly overestimated when compared to the MODIS optical cloud information. The microphysics parameterizations are found to be more sensitive than the planetary boundary layer (PBL) parameterizations.

  6. Estimates of Climate Change Impact on River Discharge in Japan Based on a Super-High-Resolution Climate Model

    Directory of Open Access Journals (Sweden)

    Yoshinobu Sato

    2012-01-01

    Full Text Available The impact of climate change on river discharge was assessed by hydrological simulations for several major river basins in Japan using the latest version of a super-high-resolution atmospheric general circulation model (AGCM with a horizontal resolution of about 20 km. Projections were made using two different datasets, one representing the present climate (1980 - 1999 and the other representing the end of the 21st century (2080 - 2099 assuming the SRES A1B scenario. River discharge was estimated by a distributed hydrological model calibrated against observed river discharge in advance. The results showed that even if the amount of precipitation does not change much in the future, river discharge will change significantly because of the increase in rainfall, decrease in snowmelt, and increase in evapotranspiration with higher air temperature. The impact of climate change on river discharge will be more significant in the northern part of Japan, especially in the Tohoku and Hokuriku regions. In these regions, the monthly average river discharge at the end of the 21st century was projected to be more than 200% higher in February and approximately 50 - 60% lower in May compared with the present flow. These results imply that the increase in air temperature has important consequences for the hydrological cycle, particularly in regions where the water supply is currently dominated by snowmelt.

  7. Overview of high-Q2 nucleon form factor program with Super BigBite Spectrometer in JLab's Hall A

    Science.gov (United States)

    Puckett, Andrew; Jefferson Lab Hall A; Super BigBite Spectrometer Collaboration

    2017-01-01

    The elastic electromagnetic form factors (EMFFs) of the nucleon describe the impact-parameter-space distributions of electric charge and magnetization in the nucleon in the infinite momentum frame. The form factors are among the simplest and most fundamental measurable dynamical quantities describing the nucleon's structure. Precision measurements of the nucleon form factors provide stringent benchmarks testing the most sophisticated theoretical models of the nucleon, as well as ab initio calculations in lattice QCD and continuum non-perturbative QCD calculations based on the Dyson-Schwinger equations. Measurements at momentum transfers Q in the few-GeV range probe the theoretically challenging region of transition between the non-perturbative and perturbative regimes of QCD. The recent upgrade of the Continuous Electron Beam Accelerator Facility (CEBAF) to a maximum electron beam energy of 11 GeV will facilitate the measurement of the nucleon helicity-conserving (F1) and helicity-flip (F2) form factors of both proton and neutron to Q2 > 10 GeV2, In this talk, I will present an overview of the Super BigBite Spectrometer, currently under construction in CEBAF's experimental Hall A, and its physics program of high-Q2 nucleon EMFF measurements. Supported by US DOE award DE-SC0014230.

  8. Comparative Study on Super Fine Mesophase Powder and MCMB Used to Manufacture High-Density Isotropic Carbon Bulks

    Institute of Scientific and Technical Information of China (English)

    LI Tong-qi; HU Zi-jun; WANG Jun-shan; GUO Yu-ming; WANG Cheng-yang

    2006-01-01

    Mesocarbon microbeads (MCMB) and super fine mesophase powder (SFMP) were prepared firstly from a coal tar pitch and then hot-condensed into high-density isotropic carbon (HDIC) bulks under 160 Mpa and finally sintered at 1 000 ℃. By analyzing the thermogravimetric behavior of the MCMB and SFMP powders, their volume shrinkage and weight loss during sintering and the bulk density and flexural strengths of their sintered bulks, it was found that the smaller sizes and the richer β-resin contents of SFMP have facilitated formation of sintered bulks with more compact isotropic structure and higher flexural strengths than MCMB. Because of the filling and bonding effects of SFMP on MCMB bulks, addition of SFMP, albeit a little, can greatly increase the flexural strengths of sintered bulks of MCMB. However, adding MCMB, even a slight amount, into SFMP can severely impair the flexural strength of sintered bulks. This might be attributed to both the crack initiation along the boundaries between MCMB and SFMP and the formation of layered texture of MCMB sphere.

  9. Changes in Some Physiological Characteristics After Full Heading Stage in Flag Leaves of Super High-Yielding Inbred Rice

    Institute of Scientific and Technical Information of China (English)

    HUANG Min; ZOU Yingbin

    2009-01-01

    In a field experiment, rice cultivar Shanyou 63 was used as a check cuitivar to investigate the changes in activity of peroxidase and contents of malondialdehyde and soluble protein after full heading stage in flag leaves of two super high-yielding inbred rice, including Shengtai 1 and Yuxiangyouzhan. The results showed that the peroxidase activities and soluble protein contents in the leaves of the three cultivars tended to increase gradually to peak values and thereafter descended during stage after full heading. Moreover, both the peroxidase activities and soluble protein contents of Shengtai 1 and Yuxiangyouzhan peaked at 8 days after full heading, which was compared with that of Shanyou 63 at 4 days later. Meanwhile, the malondialdehyde contents in the leaves of the three cultivars increased gradually after full heading stage. In addition, activities of peroxidase and contents of malondialdehyde and soluble protein in different cultivars were various at the same time. In general, for Shengtai 1 and Yuxiangyouzhan, the peroxidase activities and soluble protein contents were higher than those of Shanyou 63, whereas the malondialdehyde contents were lower compared with that of Shanyou 63. And these features could make the senescence of leaf become later and slower and photosynthetic product was more in Shengtai 1 and Yuxiangyouzhan than that in Shanyou 63.

  10. Design and Fabrication of the Superconducting Horizontal Bend Magnet for the Super High Momentum Spectrometer at Jefferson Lab

    Energy Technology Data Exchange (ETDEWEB)

    Chouhan, Shailendra S. [Michigan State University; DeKamp, Jon [Michigan State University; Burkhart, E. E, [Michigan State University; Bierwagen, J. [Michigan State University; Song, H. [Michigan State University; Zeller, Albert F. [Michigan State University; Brindza, Paul D. [JLAB; Lassiter, Steven R. [JLAB; Fowler, Michael J. [JLAB; Sun, Qiuli (Eric) [JLAB

    2015-06-01

    A collaboration exists between NSCL and JLab to design and build JLab's Super High Momentum Spectrometer (SHMS) horizontal bend magnet that allows the bending of the 12 GeV/c particles horizontally by 3° to allow SHMS to reach angles as low as 5.5°. Two full size coils have been wound and are cold tested for both magnetic and structural properties. Each coil is built from 90 layers of single-turn SSC outer conductor cable. An initial test coil with one third the turns was fabricated to demonstrate that the unique saddle shape with fully contoured ends could be wound with Rutherford superconducting cable. Learned lessons during the trial winding were integrated into the two complete full-scale coils that are now installed in the helium vessel. The fabrication of the iron yoke, cold mass, and thermal shield is complete, and assembly of the vacuum vessel is in progress. This paper presents the process and progress along with the modified magnet design to reduce the fringe field in the primary beam region and also includes the impact of the changes on coil forces and coil restraint system.

  11. Long-Term Observation of Triplex Surgery for Cataract after Phakic 6H Implantation for Super High Myopia

    Directory of Open Access Journals (Sweden)

    Xin Liu

    2016-01-01

    Full Text Available Purpose. To analyze the safety, effectiveness, and stability of triplex surgery for phakic 6H anterior chamber phakic intraocular lens explantation and phacoemulsification with in-the-bag IOL implantation for super high myopia in long-term observations. Methods. This retrospective case series evaluated 16 eyes of 10 patients who underwent triplex surgery. Best corrected visual acuity (BCVA, endothelial cell density (ECD, and associated adverse events were evaluated. Results. The mean follow-up time after the triplex surgery was 46 ± 14 months. The mean logMAR BCVA was significantly improved after triplex surgery (P = 0.047. One eye developed endophthalmitis five days postoperatively and underwent pars plana vitrectomy (PPV. Five eyes with preoperative severe endothelial cell loss developed corneal decompensation and underwent keratoplasty at a mean time of 9.4 ± 2.6 months after the triplex surgery. One eye had graft failure and underwent a second keratoplasty. The eye developed rhegmatogenous retinal detachment and underwent PPV with silicone oil 18 months later. ECD before the triplex surgery was not significantly different compared with that at last follow-up (P = 0.495 apart from these five eyes. Three eyes (18.8% developed posterior capsule opacification. Conclusions. Triplex surgery was safe and effective for phakic 6H related complicated cataracts. Early extraction before severe ECD loss is recommended.

  12. A robotic, compact, and extremely high resolution optical spectrograph for a close-in super-Earth survey

    Science.gov (United States)

    Ge, Jian; Powell, Scott; Zhao, Bo; Varosi, Frank; Ma, Bo; Sithajan, Sirinrat; Liu, Jian; Li, Rui; Grieves, Nolan; Schofield, Sidney; Avner, Louis; Jakeman, Hali; Yoder, William A.; Gittelmacher, Jakob A.; Singer, Michael A.; Muterspaugh, Matthew; Williamson, Michael; Maxwell, J. E.

    2014-08-01

    One of the most astonishing results from the HARPS and Kepler planet surveys is the recent discovery of close-in super-Earths orbiting more than half of FGKM dwarfs. This new population of exoplanets represents the most dominant class of planetary systems known to date, is totally unpredicted by the classical core-accretion disk planet formation model. High cadence and high precision Doppler spectroscopy is the key to characterize properties of this new population and constrain planet formation models. A new robotic, compact high resolution optical spectrograph, called TOU (formerly called EXPERT-III), was commissioned at the Automatic Spectroscopic Telescope (AST) at Fairborn Observatory in Arizona in July 2013 and has produced a spectral resolution of about 100,000 and simultaneous wavelength coverage of 0.38-0.9 μm with a 4kx4k back-illuminated Fairchild CCD detector. The instrument holds a very high vacuum of 1 micro torr and about 2 mK temperature stability over a month. The early on-sky RV measurements show that this instrument is approaching a Doppler precision of 1 m/s (rms) for bright reference stars (such as Tau Ceti) with 5 min exposures and better than 3 m/s (P-V, RMS~1 m/s) daily RV stability before calibration exposures are applied. A pilot survey of 20 Vsuper-Earth systems and known RV stable stars, is being launched and every star will be observed ~100 times over ~300 days time window between this summer and next spring, following up with a full survey of ~150 V< 10 FGKM dwarfs in 2015-2017.

  13. Super-High Temperature Alloys and Composites from NbW-Cr Systems

    Energy Technology Data Exchange (ETDEWEB)

    Shailendra Varma

    2008-12-31

    Nickel base superalloys must be replaced if the demand for the materials continues to rise for applications beyond 1000{sup o}C which is the upper limit for such alloys at this time. There are non-metallic materials available for such high temperature applications but they all present processing difficulties because of the lack of ductility. Metallic systems can present a chance to find materials with adequate room temperature ductility. Obviously the system must contain elements with high melting points. Nb has been chosen by many investigators which has a potential of being considered as a candidate if alloyed properly. This research is exploring the Nb-W-Cr system for the possible choice of alloys to be used as a high temperature material.

  14. A super base station based centralized network architecture for 5G mobile communication systems

    Directory of Open Access Journals (Sweden)

    Manli Qian

    2015-04-01

    Full Text Available To meet the ever increasing mobile data traffic demand, the mobile operators are deploying a heterogeneous network with multiple access technologies and more and more base stations to increase the network coverage and capacity. However, the base stations are isolated from each other, so different types of radio resources and hardware resources cannot be shared and allocated within the overall network in a cooperative way. The mobile operators are thus facing increasing network operational expenses and a high system power consumption. In this paper, a centralized radio access network architecture, referred to as the super base station (super BS, is proposed, as a possible solution for an energy-efficient fifth-generation (5G mobile system. The super base station decouples the logical functions and physical entities of traditional base stations, so different types of system resources can be horizontally shared and statistically multiplexed among all the virtual base stations throughout the entire system. The system framework and main functionalities of the super BS are described. Some key technologies for system implementation, i.e., the resource pooling, real-time virtualization, adaptive hardware resource allocation are also highlighted.

  15. Investigation of Phosphate Cement-based Binder with Super High Early Strength for Repair of Concrete

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Magnesium phosphate cement-based binder (MPB) for repair of concrete was prepared by proportionally mixing over burned MgO powder (M) with NHH2PO4 powder (P) and set modifying admixtures. It is characteristic by excellent properties such as rapid setting,high strength and high bond strength to old concrete.. The study is focused on the key factors influencing the setting time and strength of MPB, the bond property of MPB to old concrete and the kinetic feature of the hydration of MPB.

  16. Observing Ultra High Energy Cosmic Particles from Space: SEUSO, the Super Extreme Universe Space Observatory Mission

    CERN Document Server

    Santangelo, Andrea

    2009-01-01

    The experimental search for ultra high energy cosmic messengers, from $E\\sim 10^{19}$ eV to beyond $E\\sim 10^{20}$ eV, at the very end of the known energy spectrum, constitutes an extraordinary opportunity to explore a largely unknown aspect of our universe. Key scientific goals are the identification of the sources of ultra high energy particles, the measurement of their spectra and the study of galactic and local intergalactic magnetic fields. Ultra high energy particles might, also, carry evidence of unknown physics or of exotic particles relics of the early universe. To meet this challenge a significant increase in the integrated exposure is required. This implies a new class of experiments with larger acceptances and good understanding of the systematic uncertainties. Space based observatories can reach the instantaneous aperture and the integrated exposure necessary to systematically explore the ultra high energy universe. In this paper, after briefly summarising the science case of the mission, we desc...

  17. Combination of multiplex PCR with denaturing high-performance liquid chromatography for rapid detection of Mycobacterium genus and simultaneous identification of the Mycobacterium tuberculosis complex.

    Science.gov (United States)

    Chen, Ru; Gao, Xiao-Bo; Liu, Zhi-Hui; Shen, Xiao-Bing; Guo, Ai-Zhen; Duan, Yan-Yu; Liu, Zhi-Ling; Wu, Xiao-Wei; Zhu, Dao-Zhong

    2013-09-01

    A new assay with the combination of multiplex polymerase chain reaction and denaturing high-performance liquid chromatography analysis was developed for simultaneous detection of Mycobacterium genus and identification of the Mycobacterium tuberculosis complex (MTC). Targeting at genus-specific 16S rRNA sequence of Mycobacterium and specific insertion elements IS6110 and IS1081 of MTC, the assay was validated with 84 strains covering 23 mycobacteria species and 30 strains of non-mycobacteria species. No cross reactivity was observed. Clinical application was carried out on 198 specimens (155 human sputum and 43 bovine tissue samples) and compared with culture. The multiplex assay detected all culture-positive (36 in number) and 35.2% (57/162) culture-negative specimens. The molecular assay was fast that could be completed within 1 h on purified DNA, with the limit of detection as 0.8-1.6 pg per reaction on DNA template. This work provided a useful laboratory tool for rapid identification of Mycobacterium and differentiation of MTC and nontuberculous mycobacteria.

  18. High frequency A-type pulsators discovered using SuperWASP

    CERN Document Server

    Holdsworth, Daniel L; Gillon, M; Clubb, K I; Southworth, J; Maxted, P F L; Anderson, D R; Barros, S C C; Cameron, A Collier; Delrez, L; Faedi, F; Haswell, C A; Hellier, C; Horne, K; Jehin, E; Norton, A J; Pollacco, D; Skillen, I; Smith, A M S; West, R G; Wheatley, P J

    2014-01-01

    We present the results of a survey using the WASP archive to search for high frequency pulsations in F-, A- and B-type stars. Over 1.5 million targets have been searched for pulsations with amplitudes greater than 0.5 millimagnitude. We identify over 350 stars which pulsate with periods less than 30 min. Spectroscopic follow-up of selected targets has enabled us to confirm 10 new rapidly oscillating Ap stars, 13 pulsating Am stars and the fastest known $\\delta$ Scuti star. We also observe stars which show pulsations in both the high-frequency domain and in the low-frequency $\\delta$ Scuti range. This work shows the power of the WASP photometric survey to find variable stars with amplitudes well below the nominal photometric precision per observation.

  19. Super Continuum Generation at 1310nm in a Highly Nonlinear Photonic Crystal Fiber with a Minimum Anomalous Group Velocity Dispersion

    Directory of Open Access Journals (Sweden)

    Ashkan Ghanbari

    2014-12-01

    Full Text Available In the present study, we investigate the evolution of the super continuum generation (SCG through the triangular photonic crystal fiber (PCF at 1310nm by using both full-vector multi pole method (M.P.M and novel concrete algorithms: Symmetric Split-step Fourier (SSF and fourth order Runge Kutta(RK4 which is an accurate method to solve the general nonlinear Schrodinger equation (GNLSE. We propose an ideal solid-core PCF structure featuring a minimum anomalous group velocity dispersion (GVD, small higher order dispersions (HODs and enhanced nonlinearity for appropriate super continuum generation with low input pulse energies over discrete distances of the PCF. We also investigate the impact of the linear and nonlinear effects on the super continuum spectra in detail and compare the results with different status.

  20. Molecular Assisted Breeding and Adaptability Analysis of Zhongyouza 11 with Super High Oil Content

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Using a three-line system of Shaan 2A cytoplasmic male sterility, a hybrid variety rapeseed, Zhongyouza 11, was developed by the combination of traditional methods and a molecular marker-assisted breeding technique. This hybrid variety had high grain yield, high oil content, wide adaptability, and good quality. The oil content of Zhongyouza 11 is steadily above 43% in the three ecological regions along the Yangtze River valley, which is the largest winter oilseed production area in China. The highest oil content of Zhongyouza 11 reached 46.68% in the upper reaches of the Yangtze River valley in 2003-2004, which was the highest among all lines involved in the national regional rapeseed variety trials. In the regional yield trials in Hubei Province, the average yield of Zhongyouza 11 reached 2 853 kg ha-1 (2002-2004), yielding more than the control variety Zhongshuang 6 by 11.34%. In the national trials, the yield of Zhongyouza 11 reached 2 405.7, 2 697.3, and 2 770.2 kg ha-1 in the upper, middle, and lower reaches of the Yangtze River valley, yielding more than the control varieties by 11.52, 12.9, and 14.92%, respectively, in 2003-2005. Both the high oil content and high grain yield contributed to the high oil yield of Zhongyouza 11. The oil yield of Zhongyouza 11 in the national trials reached 1083.3, 1210.2, and 1224.8 kg ha-1, yielding more than the controls by 29.42, 27.65, and 20.98%, respectively, in the upper, middle, and lower reaches of the Yangtze River valley. The highest oil yield reached 1369.65 kg ha-1 in the middle reach of the Yangtze River valley(2003-2004), with an increase of 46.21% compared to the control. The experimental sites distributed widely along the Yangtze River valley, cover most of the Chinese winter rapeseed production areas. Among the 89 experimental location/years, the yield of Zhongyouza 11 surpassed the controls at 77 location/years in the trials, indicating its wide adaptability.Zhongyouza 11 was the only one that passed the

  1. Reliability of system identification technique in super high-rise building

    Directory of Open Access Journals (Sweden)

    Ayumi eIkeda

    2015-07-01

    Full Text Available A smart physical-parameter based system identification method has been proposed in the previous paper. This method deals with time-variant nonparametric identification of natural frequencies and modal damping ratios using ARX (Auto-Regressive eXogenous models and has been applied to high-rise buildings during the 2011 off the Pacific coast of Tohoku earthquake. In this perspective article, the current state of knowledge in this class of system identification methods is explained briefly and the reliability of this smart method is discussed through the comparison with the result by a more confident technique.

  2. Cultivar and Tree Density As Key Factors in the Long-Term Performance of Super High-Density Olive Orchards.

    Science.gov (United States)

    Díez, Concepción M; Moral, Juan; Cabello, Diego; Morello, Pablo; Rallo, Luis; Barranco, Diego

    2016-01-01

    Super high-density (SHD) olive orchards are rapidly expanding since the first plantation was set up in Spain in the 1990s. Because there are no long-term studies characterizing these systems, it is unknown if densities above a certain threshold could trigger competition among fully-grown trees, compromising their development. Over 14 years we have evaluated the performance of the major olive cultivars currently planted in SHD systems ("Arbequina," Arbequina IRTA-i·18, "Arbosana," "Fs-17," and "Koroneiki") and nine SHD designs ranging from 780 to 2254 trees ha(-1) for the cultivar "Arbequina." Remarkably, the accumulated fruit and oil production of the five cultivars increased linearly over time. Our data indicated the favorable long-term performance of the evaluated cultivars with an average annual oil production of 2.3 t ha(-1). Only "Fs-17" did not perform well to the SHD system in our conditions and it yielded about half (1.2 t ha(-1)) of the other cultivars. In the density trial for "Arbequina," both fruit and oil accumulated production increased over time as a function of tree density. Thus, the accumulated oil yield ranged from 16.1 t ha(-1) for the lowest density (780 trees ha(-1)) to 29.9 t ha(-1) for the highest (2254 trees ha(-1)). In addition, we note that the accumulated production per surface unit showed a better correlation with the hedgerow length than the tree density. Thus, the current planting designs of SHD olive orchards can be further improved taking this parameter into account. Despite observations that some irregular patterns of crop distribution have arisen, our olive hedgerows are still fully productive after 14 years of planting. This result contradicts previous experiences that showed declines in production 7 or 8 years after planting due to high vigor, shading, and limited ventilation.

  3. Super-High Heat Flux Removal Using Sintered Metal Porous Media

    Institute of Scientific and Technical Information of China (English)

    Kazuhisa YUKI; Jun ABEI; Hidetoshi HASHIZUME; Saburo TODA

    2005-01-01

    Heat transfer experiments using various porous materials are performed to verify the possibility of extremely highheat flux removal (several dozens of MW/m2). The porous media introduced in this experiment are bronze particle-sintered ones and copper fibers-sintered ones that have high thermal conductivity, each of which can enhance the thermal diffusion in the porous material. From the heat transfer experiments using a non-transferredtype of plasma arcjet as a high heat flux source, the steady heat removal of 66.0 MW/m2 is proved by using the bronze porous medium whose pore size and porosity are 100 μm and 0.38, respectively. Furthermore, as the result of eveluating the heat removal performance for various copper fiber porous media with the porosity from 0.5 to 0.7 and the fiber diameter from 30 to 90 μm, it is clarified that the maximum cooling performance exceeds 70.0MW/m2 at the porosity of 0.7 and the fiber diameter of 90 μm, and that using the lower porosity material is useful for improving an evaporation rate in the porous media.

  4. High-magnification super-resolution FINCH microscopy using birefringent crystal lens interferometers

    Science.gov (United States)

    Siegel, Nisan; Lupashin, Vladimir; Storrie, Brian; Brooker, Gary

    2016-12-01

    Fresnel incoherent correlation holography (FINCH) microscopy is a promising approach for high-resolution biological imaging but has so far been limited to use with low-magnification, low-numerical-aperture configurations. We report the use of in-line incoherent interferometers made from uniaxial birefringent α-barium borate (α-BBO) or calcite crystals that overcome the aberrations and distortions present with previous implementations that employed spatial light modulators or gradient refractive index lenses. FINCH microscopy incorporating these birefringent elements and high-numerical-aperture oil immersion objectives could outperform standard wide-field fluorescence microscopy, with, for example, a 149 nm lateral point spread function at a wavelength of 590 nm. Enhanced resolution was confirmed with sub-resolution fluorescent beads. Taking the Golgi apparatus as a biological example, three different proteins labelled with GFP and two other fluorescent dyes in HeLa cells were resolved with an image quality that is comparable to similar samples captured by structured illumination microscopy.

  5. Super-aperture metrology: overcoming a fundamental limit in imaging smooth highly curved surfaces.

    Science.gov (United States)

    Liu, J; Liu, C; Tan, J; Yang, B; Wilson, T

    2016-03-01

    The imaging of smooth, highly curved or tilted surfaces is widely recognized as one of the most challenging and unsolved problems in optical imaging and metrology today. The reason is that even when such surfaces are imaged using high aperture microscope objectives the steepness of the features causes the light to be reflected in such a way that it is not captured by the lens. This is true even in the limiting case of unity numerical aperture since the illuminating light may also be reflected in the forward direction. In order to overcome this fundamental problem we have developed a method whereby such specimens are covered with a readily removable organic fluorescent film thereby creating an isotropic scattering surface. We show that we are readily able to detect slopes with angles close 90° using a 0.75 NA objective--an 82% improvement over the theoretical aperture limit. Issues of variation in film thickness deposition are shown to be readily accommodated. This approach may be used with other fluorophore materials, organic or inorganic, since there is no need for biocompatibility in this application.

  6. Super-sparse principal component analyses for high-throughput genomic data

    Directory of Open Access Journals (Sweden)

    Lee Youngjo

    2010-06-01

    Full Text Available Abstract Background Principal component analysis (PCA has gained popularity as a method for the analysis of high-dimensional genomic data. However, it is often difficult to interpret the results because the principal components are linear combinations of all variables, and the coefficients (loadings are typically nonzero. These nonzero values also reflect poor estimation of the true vector loadings; for example, for gene expression data, biologically we expect only a portion of the genes to be expressed in any tissue, and an even smaller fraction to be involved in a particular process. Sparse PCA methods have recently been introduced for reducing the number of nonzero coefficients, but these existing methods are not satisfactory for high-dimensional data applications because they still give too many nonzero coefficients. Results Here we propose a new PCA method that uses two innovations to produce an extremely sparse loading vector: (i a random-effect model on the loadings that leads to an unbounded penalty at the origin and (ii shrinkage of the singular values obtained from the singular value decomposition of the data matrix. We develop a stable computing algorithm by modifying nonlinear iterative partial least square (NIPALS algorithm, and illustrate the method with an analysis of the NCI cancer dataset that contains 21,225 genes. Conclusions The new method has better performance than several existing methods, particularly in the estimation of the loading vectors.

  7. A novel IPTV program multiplex access system to EPON

    Science.gov (United States)

    Xu, Xian; Liu, Deming; He, Wei; Lu, Xi

    2007-11-01

    With the rapid development of high speed networks, such as Ethernet Passive Optical Network (EPON), traffic patterns in access networks have evolved from traditional text-oriented service to the mixed text-, voice- and video- based services, leading to so called "Triple Play". For supporting IPTV service in EPON access network infrastructure, in this article we propose a novel IPTV program multiplex access system to EPON, which enables multiple IPTV program source servers to seamlessly access to IPTV service access port of optical line terminal (OLT) in EPON. There are two multiplex schemes, namely static multiplex scheme and dynamic multiplex scheme, in implementing the program multiplexing. Static multiplex scheme is to multiplex all the IPTV programs and forward them to the OLT, regardless of the need of end-users. While dynamic multiplex scheme can dynamically multiplex and forward IPTV programs according to what the end-users actually demand and those watched by no end-user would not be multiplexed. By comparing these two schemes, a reduced traffic of EPON can be achieved by using dynamic multiplex scheme, especially when most end-users are watching the same few IPTV programs. Both schemes are implemented in our system, with their hardware and software designs described.

  8. Unexpected generation of super-high energetic electrons at relativistic circularly polarized laser-solid interactions in the presence of large scale pre-plasmas

    CERN Document Server

    Wu, D; Luan, S X; Yu, W

    2015-01-01

    As a continuation of the previous work "Identifying the source of super-high energetic electrons in the presence of pre-plasma in laser-matter interaction at relativistic intensities [arXiv: 1512.02411]", we have investigated the role of circularly polarized (CP) laser pulses while keeping other conditions the same. It is found that in the presence of large scale pre-formed plasmas, super-high energetic electrons can be generated at relativistic CP laser-solid interactions. For laser of intensity 10$^{20}\\ $W$/$cm$^2$ and pre-plasma scale-length 10$\\ \\mu$m, the cut-off energy of electron by CP laser is 120$\\ $MeV compared with 100$\\ $MeV in the case of linearly polarized (LP) laser. The unexpected super-high energetic electron acceleration can also be explained by the two-stage acceleration model, by considering the polarization transition of the reflected laser from CP to elliptically polarized (EP). The polarization state transition is addressed, and a modified scaling law in the presence of EP laser is obt...

  9. High resolution OCT image generation using super resolution via sparse representation

    Science.gov (United States)

    Asif, Muhammad; Akram, Muhammad Usman; Hassan, Taimur; Shaukat, Arslan; Waqar, Razi

    2017-02-01

    In this paper we propose a technique for obtaining a high resolution (HR) image from a single low resolution (LR) image -using joint learning dictionary - on the basis of image statistic research. It suggests that with an appropriate choice of an over-complete dictionary, image patches can be well represented as a sparse linear combination. Medical imaging for clinical analysis and medical intervention is being used for creating visual representations of the interior of a body, as well as visual representation of the function of some organs or tissues (physiology). A number of medical imaging techniques are in use like MRI, CT scan, X-rays and Optical Coherence Tomography (OCT). OCT is one of the new technologies in medical imaging and one of its uses is in ophthalmology where it is being used for analysis of the choroidal thickness in the eyes in healthy and disease states such as age-related macular degeneration, central serous chorioretinopathy, diabetic retinopathy and inherited retinal dystrophies. We have proposed a technique for enhancing the OCT images which can be used for clearly identifying and analyzing the particular diseases. Our method uses dictionary learning technique for generating a high resolution image from a single input LR image. We train two joint dictionaries, one with OCT images and the second with multiple different natural images, and compare the results with previous SR technique. Proposed method for both dictionaries produces HR images which are comparatively superior in quality with the other proposed method of SR. Proposed technique is very effective for noisy OCT images and produces up-sampled and enhanced OCT images.

  10. SuperSegger

    DEFF Research Database (Denmark)

    Stylianidou, Stella; Brennan, Connor; Nissen, Silas B

    2016-01-01

    -colonies with many cells, facilitating the analysis of cell-cycle dynamics in bacteria as well as cell-contact mediated phenomena. This package has a range of built-in capabilities for characterizing bacterial cells, including the identification of cell division events, mother, daughter, and neighboring cells......Many quantitative cell biology questions require fast yet reliable automated image segmentation to identify and link cells from frame-to-frame, and characterize the cell morphology and fluorescence. We present SuperSegger, an automated MATLAB-based image processing package well......-suited to quantitative analysis of high-throughput live-cell fluorescence microscopy of bacterial cells. SuperSegger incorporates machine-learning algorithms to optimize cellular boundaries and automated error resolution to reliably link cells from frame-to-frame. Unlike existing packages, it can reliably segment micro...

  11. Super-Hydrophobic High Throughput Electrospun Cellulose Acetate (CA) Nanofibrous Mats as Oil Selective Sorbents

    Science.gov (United States)

    Han, Chao

    The threat of oil pollution increases with the expansion of oil exploration and production activities, as well as the industrial growth around the world. Use of sorbents is a common method to deal with the oil spills. In this work, an advanced sorbent technology is described. A series of non-woven Cellulose Acetate (CA) nanofibrous mats with a 3D fibrous structure were synthesized by a novel high-throughput electrospinning technique. The precursor was solutions of CA/ acetic acid-acetone in various concentrations. Among them, 15.0% CA exhibits a superhydrophobic surface property, with a water contact angle of 128.95°. Its oil sorption capacity is many times higher the oil sorption capacity of the best commercial sorbent available in the market. Also, it showed good buoyancy properties on the water both as dry-mat and oil-saturated mat. In addition, it is biodegradable, easily available, easily manufactured, so the CA nanofibrous mat is an excellent candidate as oil sorbent for oil spill in water treatment.

  12. Becoming a high-fidelity - super - imitator: what are the contributions of social and individual learning?

    Science.gov (United States)

    Subiaul, Francys; Patterson, Eric M; Schilder, Brian; Renner, Elizabeth; Barr, Rachel

    2015-11-01

    In contrast to other primates, human children's imitation performance goes from low to high fidelity soon after infancy. Are such changes associated with the development of other forms of learning? We addressed this question by testing 215 children (26-59 months) on two social conditions (imitation, emulation) - involving a demonstration - and two asocial conditions (trial-and-error, recall) - involving individual learning - using two touchscreen tasks. The tasks required responding to either three different pictures in a specific picture order (Cognitive: Airplane→Ball→Cow) or three identical pictures in a specific spatial order (Motor-Spatial: Up→Down→Right). There were age-related improvements across all conditions and imitation, emulation and recall performance were significantly better than trial-and-error learning. Generalized linear models demonstrated that motor-spatial imitation fidelity was associated with age and motor-spatial emulation performance, but cognitive imitation fidelity was only associated with age. While this study provides evidence for multiple imitation mechanisms, the development of one of those mechanisms - motor-spatial imitation - may be bootstrapped by the development of another social learning skill - motor-spatial emulation. Together, these findings provide important clues about the development of imitation, which is arguably a distinctive feature of the human species.

  13. Characterising Super-Earths

    Directory of Open Access Journals (Sweden)

    Valencia D.

    2011-02-01

    Full Text Available The era of Super-Earths has formally begun with the detection of transiting low-mass exoplanets CoRoT-7b and GJ 1214b. In the path of characterising super-Earths, the first step is to infer their composition. While the discovery data for CoRoT-7b, in combination with the high atmospheric mass loss rate inferred from the high insolation, suggested that it was a rocky planet, the new proposed mass values have widened the possibilities. The combined mass range 1−10 M⊕ allows for a volatile-rich (and requires it if the mass is less than 4 M⊕ , an Earth-like or a super-Mercury-like composition. In contrast, the radius of GJ 1214b is too large to admit a solid composition, thus it necessarily to have a substantial gas layer. Some evidence suggests that within this gas layer H/He is a small but non-negligible component. These two planets are the first of many transiting low-mass exoplanets expected to be detected and they exemplify the limitations faced when inferring composition, which come from the degenerate character of the problem and the large error bars in the data.

  14. Super-TIGER2: A Very-Large-Area, High-Resolution, Trans-Iron Cosmic Ray Investigation - Caltech Co-I

    Science.gov (United States)

    Mewaldt, Richard

    This is a Co-I proposal from the California Institute of Technology for the proposal entitled “SuperTIGER-2: A Very-Large-Area, High-Resolution Trans-Iron Cosmic Ray Investigation” submitted by Principal Investigator W. R. Binns of Washington University in St. Louis. SuperTIGER is a very-large-area instrument for measuring the composition of galactic cosmic rays on high-altitude balloon flights over Antarctica. Super-TIGER-1 had a highly successful 55-day flight in 2012/2013. The excellent data quality enables us to clearly resolve all nuclei from Z=10 to Z=40. Additionally, although statistics are low, there appears to be clear resolution of elements with Z=50-60. The excellent data from this flight will enable us to achieve the initial goals of the program. The high performance of the instrument, both in charge resolution and collecting power, and the science that can be addressed by measurements of Z>40 nuclei, makes a compelling case to conduct additional flights to measure the abundances of individual nuclei up to Z=60. This is a 4- year proposal with the primary objective of measuring the abundances of individual nuclei with 41#Z#60 and to substantially increase the number of 30#Z#40 nuclei measured. This will be the first time that individual elemental abundances for 41#Z#60 will be obtained. These new measurements will provide sensitive tests and clarification of the OB-association model of galactic cosmic-ray origins and will test models for atomic processes by which nuclei are selected for acceleration to cosmic-ray energies. They will enable us to determine if the enrichment of refractory elements (those that exist primarily in dust grains in the interstellar medium) over volatile elements (those that exist primarily in the gas phase) extends into the Z=41-60 charge range. They will also enable us to unambiguously determine the extent of any r-process enrichment. SuperTIGER provides critical measurements to unravel the mystery of galactic cosmic ray

  15. Discussion on super-tall structures type selection in high wind load area%谈高风载地区超高层结构选型

    Institute of Scientific and Technical Information of China (English)

    任军

    2016-01-01

    结合珠海某超高层建筑的结构设计参数,建立了四种结构体系,对不同的结构选型进行了分析比较,得到了不同结构形式的优缺点,为高风载地区超高层结构方案设计提供了参考依据。%Combining with the structure design parameters of a super-tall building in Zhuhai established four kinds of structure system,compared and analyzed different structure type selection,gained the advantages and disadvantages of different structure forms,provided reference basis for super-tall structures scheme design in high wind load areas.

  16. High-throughput droplet analysis and multiplex DNA detection in the microfluidic platform equipped with a robust sample-introduction technique

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Jinyang; Ji, Xinghu [Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); He, Zhike, E-mail: zhkhe@whu.edu.cn [Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); Suzhou Institute of Wuhan University, Suzhou 215123 (China)

    2015-08-12

    In this work, a simple, flexible and low-cost sample-introduction technique was developed and integrated with droplet platform. The sample-introduction strategy was realized based on connecting the components of positive pressure input device, sample container and microfluidic chip through the tygon tubing with homemade polydimethylsiloxane (PDMS) adaptor, so the sample was delivered into the microchip from the sample container under the driving of positive pressure. This sample-introduction technique is so robust and compatible that could be integrated with T-junction, flow-focus or valve-assisted droplet microchips. By choosing the PDMS adaptor with proper dimension, the microchip could be flexibly equipped with various types of familiar sample containers, makes the sampling more straightforward without trivial sample transfer or loading. And the convenient sample changing was easily achieved by positioning the adaptor from one sample container to another. Benefiting from the proposed technique, the time-dependent concentration gradient was generated and applied for quantum dot (QD)-based fluorescence barcoding within droplet chip. High-throughput droplet screening was preliminarily demonstrated through the investigation of the quenching efficiency of ruthenium complex to the fluorescence of QD. More importantly, multiplex DNA assay was successfully carried out in the integrated system, which shows the practicability and potentials in high-throughput biosensing. - Highlights: • A simple, robust and low-cost sample-introduction technique was developed. • Convenient and flexible sample changing was achieved in microfluidic system. • Novel strategy of concentration gradient generation was presented for barcoding. • High-throughput droplet screening could be realized in the integrated platform. • Multiplex DNA assay was successfully carried out in the droplet platform.

  17. Multiplexed DNA-modified electrodes.

    Science.gov (United States)

    Slinker, Jason D; Muren, Natalie B; Gorodetsky, Alon A; Barton, Jacqueline K

    2010-03-03

    We report the use of silicon chips with 16 DNA-modified electrodes (DME chips) utilizing DNA-mediated charge transport for multiplexed detection of DNA and DNA-binding protein targets. Four DNA sequences were simultaneously distinguished on a single DME chip with 4-fold redundancy, including one incorporating a single base mismatch. These chips also enabled investigation of the sequence-specific activity of the restriction enzyme Alu1. DME chips supported dense DNA monolayer formation with high reproducibility, as confirmed by statistical comparison to commercially available rod electrodes. The working electrode areas on the chips were reduced to 10 microm in diameter, revealing microelectrode behavior that is beneficial for high sensitivity and rapid kinetic analysis. These results illustrate how DME chips facilitate sensitive and selective detection of DNA and DNA-binding protein targets in a robust and internally standardized multiplexed format.

  18. What are super-enhancers?

    Science.gov (United States)

    Pott, Sebastian; Lieb, Jason D

    2015-01-01

    The term 'super-enhancer' has been used to describe groups of putative enhancers in close genomic proximity with unusually high levels of Mediator binding, as measured by chromatin immunoprecipitation and sequencing (ChIP-seq). Here we review the identification and composition of super-enhancers, describe links between super-enhancers, gene regulation and disease, and discuss the functional significance of enhancer clustering. We also provide our perspective regarding the proposition that super-enhancers are a regulatory entity conceptually distinct from what was known before the introduction of the term. Our opinion is that there is not yet strong evidence that super-enhancers are a novel paradigm in gene regulation and that use of the term in this context is not currently justified. However, the term likely identifies strong enhancers that exhibit behaviors consistent with previous models and concepts of transcriptional regulation. In this respect, the super-enhancer definition is useful in identifying regulatory elements likely to control genes important for cell type specification.

  19. Multiplex tokamak power plant

    Energy Technology Data Exchange (ETDEWEB)

    Dabiri, A.E.

    1986-07-01

    The concept of multiplexing for a fusion power core as an option for producing power is explored. Superconducting, as well as normal magnet, coils in either first or second stability regimes are considered. The results show that multiplex plants with superconducting magnets operating in the second stability regime could be competitive with the single-unit plants in some unit sizes. The key issues that impact the expected benefits of multiplexing must be investigated further. These are factory fabrication, economy of scale, the extent of equipment sharing, inherent safety, maintainability, and utility load management.

  20. Rapid high-throughput analysis of DNaseI hypersensitive sites using a modified Multiplex Ligation-dependent Probe Amplification approach

    Directory of Open Access Journals (Sweden)

    Sinclair Andrew H

    2009-09-01

    Full Text Available Abstract Background Mapping DNaseI hypersensitive sites is commonly used to identify regulatory regions in the genome. However, currently available methods are either time consuming and laborious, expensive or require large numbers of cells. We aimed to develop a quick and straightforward method for the analysis of DNaseI hypersensitive sites that overcomes these problems. Results We have developed a modified Multiplex Ligation-dependent Probe Amplification (MLPA approach for the identification and analysis of genomic regulatory regions. The utility of this approach was demonstrated by simultaneously analysing 20 loci from the ENCODE project for DNaseI hypersensitivity in a range of different cell lines. We were able to obtain reproducible results with as little as 5 × 104 cells per DNaseI treatment. Our results broadly matched those previously reported by the ENCODE project, and both technical and biological replicates showed high correlations, indicating the sensitivity and reproducibility of this method. Conclusion This new method will considerably facilitate the identification and analysis of DNaseI hypersensitive sites. Due to the multiplexing potential of MLPA (up to 50 loci can be examined it is possible to analyse dozens of DNaseI hypersensitive sites in a single reaction. Furthermore, the high sensitivity of MLPA means that fewer than 105 cells per DNaseI treatment can be used, allowing the discovery and analysis of tissue specific regulatory regions without the need for pooling. This method is quick and easy and results can be obtained within 48 hours after harvesting of cells or tissues. As no special equipment is required, this method can be applied by any laboratory interested in the analysis of DNaseI hypersensitive regions.

  1. Watching the Birth of Super Star Clusters

    CERN Document Server

    Turner, J L; Turner, Jean L.; Beck, Sara C.

    2003-01-01

    Subarcsecond infrared and radio observations yield important information about the formation of super star clusters from their surrounding gas. We discuss the general properties of ionized and molecular gas near young, forming SSCs, as illustrated by the prototypical young forming super star cluster nebula in the dwarf galaxy, NGC 5253. This super star cluster appears to have a gravitationally bound nebula. The lack of molecular gas suggests a very high star formation efficiency, consistent with the formation of a large, bound star cluster.

  2. High sensitivity measurement of NO, NO2 and NH3 using MIR-QCL and time division multiplexing WMS technology

    Science.gov (United States)

    Chen, Xiang; Yang, Chenguang; Hu, Mai; Xu, Zhenyu; Fan, Xueli; Wei, Min; Yao, Lu; He, Yabai; Kan, Ruifeng

    2016-10-01

    A compact system based on mid-infrared quantum cascade laser (QCL) operated in room temperature was developed for the simultaneous monitoring of NO, NO2 and NH3 in the air. Laser beams of three QCLs with central wavelength located at 1900 cm-1, 1600 cm-1, 1103.4 cm-1 were coupled to pass through the 60m long gas cell together. With the technology of time division multiplexing, wavelength modulation spectroscopy (WMS) signals of three lasers can be detected at adjacent scan process. The real-time second harmonic analysis was implemented to achieve simultaneous detection of NO, NO2 and NH3. A minimum detection limit (MDL) of 0.2ppb for NO, 0.12ppb for NO2 and 0.1ppb for NH3 with an optimum integration time around 100 seconds can be achieved for this setup. An ambient monitoring of three gasses during 5 hours was performed to inspect the local air quality.

  3. Additively manufactured MEMS multiplexed coaxial electrospray sources for high-throughput, uniform generation of core-shell microparticles.

    Science.gov (United States)

    Olvera-Trejo, D; Velásquez-García, L F

    2016-10-18

    This study reports the first MEMS multiplexed coaxial electrospray sources in the literature. Coaxial electrospraying is a microencapsulation technology based on electrohydrodynamic jetting of two immiscible liquids, which allows precise control with low size variation of the geometry of the core-shell particles it generates, which is of great importance in numerous biomedical and engineering applications, e.g., drug delivery and self-healing composites. By implementing monolithic planar arrays of miniaturized coaxial electrospray emitters that work uniformly in parallel, the throughput of the compound microdroplet source is greatly increased, making the microencapsulation technology compatible with low-cost commercial applications. Miniaturized core-shell particle generators with up to 25 coaxial electrospray emitters (25 emitters cm(-2)) were fabricated via stereolithography, which is an additive manufacturing process that can create complex microfluidic devices at a small fraction of the cost per device and fabrication time associated with silicon-based counterparts. The characterization of devices with the same emitter structure but different array sizes demonstrates uniform array operation. Moreover, the data demonstrate that the per-emitter current is approximately proportional to the square root of the flow rate of the driving liquid, and it is independent of the flow rate of the driven liquid, as predicted by the theory. The core/shell diameters and the size distribution of the generated compound microparticles can be modulated by controlling the flow rates fed to the emitters.

  4. Cultivar and Tree Density As Key Factors in the Long-Term Performance of Super High-Density Olive Orchards

    Science.gov (United States)

    Díez, Concepción M.; Moral, Juan; Cabello, Diego; Morello, Pablo; Rallo, Luis; Barranco, Diego

    2016-01-01

    Super high-density (SHD) olive orchards are rapidly expanding since the first plantation was set up in Spain in the 1990s. Because there are no long-term studies characterizing these systems, it is unknown if densities above a certain threshold could trigger competition among fully-grown trees, compromising their development. Over 14 years we have evaluated the performance of the major olive cultivars currently planted in SHD systems (“Arbequina,” Arbequina IRTA-i·18, “Arbosana,” “Fs-17,” and “Koroneiki”) and nine SHD designs ranging from 780 to 2254 trees ha−1 for the cultivar “Arbequina.” Remarkably, the accumulated fruit and oil production of the five cultivars increased linearly over time. Our data indicated the favorable long-term performance of the evaluated cultivars with an average annual oil production of 2.3 t ha−1. Only “Fs-17” did not perform well to the SHD system in our conditions and it yielded about half (1.2 t ha−1) of the other cultivars. In the density trial for “Arbequina,” both fruit and oil accumulated production increased over time as a function of tree density. Thus, the accumulated oil yield ranged from 16.1 t ha−1 for the lowest density (780 trees ha−1) to 29.9 t ha−1 for the highest (2254 trees ha−1). In addition, we note that the accumulated production per surface unit showed a better correlation with the hedgerow length than the tree density. Thus, the current planting designs of SHD olive orchards can be further improved taking this parameter into account. Despite observations that some irregular patterns of crop distribution have arisen, our olive hedgerows are still fully productive after 14 years of planting. This result contradicts previous experiences that showed declines in production 7 or 8 years after planting due to high vigor, shading, and limited ventilation. PMID:27602035

  5. High-risk human papillomavirus infection involving multiple anatomic sites of the female lower genital tract: a multiplex real-time polymerase chain reaction-based study.

    Science.gov (United States)

    Hui, Yiang; Manna, Pradip; Ou, Joyce J; Kerley, Spencer; Zhang, Cunxian; Sung, C James; Lawrence, W Dwayne; Quddus, M Ruhul

    2015-09-01

    High-risk human papillomavirus infection usually is seen at one anatomic site in an individual. Rarely, infection at multiple anatomic sites of the female lower genital tract in the same individual is encountered either simultaneously and/or at a later date. The current study identifies the various subtypes of high-risk human papillomavirus infection in these scenarios and analyzes the potential significance of these findings. High-risk human papillomavirus infection involving 22 anatomic sites from 7 individuals was identified after institutional review board approval. Residual paraffin-embedded tissue samples were retrieved, and all 15 high-risk human papillomavirus were identified and viral load quantified using multiplex real-time polymerase chain reaction-based method. Multiple high-risk human papillomavirus subtypes were identified in 32% of the samples and as many as 5 different subtypes of high-risk human papillomavirus infection in a single anatomic site. In general, each anatomic site has unique combination of viral subtypes, although one individual showed overlapping subtypes in the vagina, cervix, and vulvar samples. Higher viral load and rare subtypes are more frequent in younger patients and in dysplasia compared with carcinoma. Follow-up ranging from 3 to 84 months revealed persistent high-risk human papillomavirus infection in 60% of cases. Copyright © 2015 Elsevier Inc. All rights reserved.

  6. Multiplexed lasing in tissues

    Science.gov (United States)

    Chen, Yu-Cheng; Chen, Qiushu; Fan, Xudong

    2017-02-01

    Biolasers are an emerging technology for next generation biochemical detection and clinical applications. Progress has recently been made to achieve lasing from biomolecules and single living cells. Tissues, which consist of cells embedded in extracellular matrix, mimic more closely the actual complex biological environment in a living body and therefore are of more practical significance. Here, we developed a highly versatile tissue laser platform, in which tissues stained with fluorophores are sandwiched in a high-Q Fabry-Pérot microcavity. Distinct lasing emissions from muscle and adipose tissues stained respectively with fluorescein isothiocyanate (FITC) and boron-dipyrromethene (BODIPY), and hybrid muscle/adipose tissue with dual-staining were achieved with a threshold of only 10 μJ/mm2. Additionally, we investigated how tissue structure/geometry, tissue thickness, and staining dye concentration affect the tissue laser. It is further found that, despite large fluorescence spectral overlap between FITC and BODIPY in tissues, their lasing emissions could be clearly distinguished and controlled due to their narrow lasing bands and different lasing thresholds, thus enabling highly multiplexed detection. Our tissue laser platform can be broadly applicable to various types of tissues/diseases. It provides a new tool for a wide range of biological and biomedical applications, such as diagnostics/screening of tissues and identification/monitoring of biological transformations in tissue engineering.

  7. AlPO4-C Composite Coating on Ni-based Super Alloy Substrates for High Emissivity Applications : Experimentation on Dip Coating and Spray Coating

    OpenAIRE

    Subir Roy; S. Rangaswamy Reddy; Sindhuja, P; Dipak Das; V.V. Bhauprasad

    2016-01-01

    High emissivity coating was developed on Ni-based super alloy substrates by dip coating and spray coating technique using a chemical precursor sol. The coating material was characterised thoroughly by XRD, SEM, TEM and XPS analyses. Characterisation results showed the presence of nano carbon in the AlPO4 matrix which imparted high emissivity to the coating. Emissivity of the coating varied from 0.6 to 0.9 in the wave length range : 2 µm - 25 µm depending on the thickness of the multilayered c...

  8. New method for making super-plastic glasses

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ It was a long-cherished dream for materials scientists to find a nearly ideal metallic alloy with high strength and super-plasticity concurrently as a super-material both extremely strong and exceptionally hard for human use.

  9. Development and Challenges of Super High Dams in China and Other Countries%我国超级高坝的发展与挑战

    Institute of Scientific and Technical Information of China (English)

    王爱玲; 邓正刚

    2015-01-01

    The statistics on high dams with a height more than 200 m in abroad and home under operation, construction or planning shows that the super high dam construct earlier and continues to develop in foreign countries, and starts later in but rapid in China. The super high dams mainly belong to concrete arch dam, concrete gravity dam, clay core wall rockfill dam or concrete face rockfill dam, and all super high dams which have been put into operation can safely and reliably operate. The development and characteristics of hydropower engineering in China are analyzed and the result shows the development of super high dams in China will face the challenges of high safety requirement, lack of relevant technical standards, joint research requirements on key technologies, risk prevention and control and effective management in whole operation life.%对国内外已建、在建和拟建200 m以上超级高坝进行统计表明,国外超级高坝建设起步较早,发展持续不断,我国起步较晚,但发展较快;超级高坝主要集中在混凝土拱坝、混凝土重力坝、土心墙堆石坝和混凝土面板堆石坝等4种坝型,已建工程都能安全可靠运行。通过分析我国水电工程发展趋势以及工程特点,我国超级高坝的发展面临安全要求高、相关技术标准缺乏、关键技术需联合攻关、风险防控和全寿命周期的有效管理等方面的挑战。

  10. Low-Voltage MOS Current Mode Logic Multiplexer

    Directory of Open Access Journals (Sweden)

    K. Gupta

    2013-04-01

    Full Text Available In this paper, a new low-voltage MOS current mode logic (MCML multiplexer based on the triple-tail cell concept is proposed. An analytical model for static parameters is formulated and is applied to develop a design approach for the proposed low-voltage MCML multiplexer. The delay of the proposed low-voltage MCML multiplexer is expressed in terms of the bias current and the voltage swing so that it can be traded off with the power consumption. The proposed low-voltage MCML multiplexer is analyzed for the three design cases namely high-speed, power-efficient, and low-power. Finally, a comparison in performance of the proposed low-voltage MCML multiplexer with the traditional MCML multiplexer is carried out for all the cases.

  11. Study on the Practice and High-yielding Mechanism of Super-sparse-cultivation Associated with Maximum-tiller Seedling of Hybrid Rice

    Institute of Scientific and Technical Information of China (English)

    MA Jun; TAO Shi-shun

    2002-01-01

    In this paper, a new cultivation practice-super-sparse-cultivation associated with maximumtiller seedling (SSCMTS) of hybrid rice was proposed and its high-yielding mechanism was studied. The results showed that the practice of SSCMTS in hybrid rice could not only increase grain yield but also save seeds and labor. It was a new high-yielding way for the late transplanting seedlings and heavy panicle type hybrid rice cultivars to further utilize the high-yielding potential of hybrid rice cultivars. The increasing number of spikelets and ideal grain -filling were the direct factors for the high yield of SSCMTS in hybrid rice, and those high-yielding factors relied on high quality seedlings, sturdy individuals, high quality population and vigorous later growth.

  12. Super insulating aerogel glazing

    DEFF Research Database (Denmark)

    Schultz, Jørgen Munthe; Jensen, Karsten Ingerslev; Kristiansen, Finn Harken

    2004-01-01

    Monolithic silica aerogel offers the possibility of combining super insulation and high solar energy transmittance, which has been the background for a previous and a current EU project on research and development of monolithic silica aerogel as transparent insulation in windows. Generally, windows...... form the weakest part of the thermal envelope with respect to heat loss coefficient, but on the other hand also play an important role for passive solar energy utilisation. For window orientations other than south, the net energy balance will be close to or below zero. However, the properties...

  13. glyXalign: high-throughput migration time alignment preprocessing of electrophoretic data retrieved via multiplexed capillary gel electrophoresis with laser-induced fluorescence detection-based glycoprofiling.

    Science.gov (United States)

    Behne, Alexander; Muth, Thilo; Borowiak, Matthias; Reichl, Udo; Rapp, Erdmann

    2013-08-01

    Glycomics has become a rapidly emerging field and monitoring of protein glycosylation is needed to ensure quality and consistency during production processes of biologicals such as therapeutic antibodies or vaccines. Glycoanalysis via multiplexed CGE with LIF detection (xCGE-LIF) represents a powerful technique featuring high resolution, high sensitivity as well as high-throughput performance. However, sample data retrieved from this method exhibit challenges for downstream computational analysis due to intersample migration time shifts as well as stretching and compression of electropherograms. Here, we present glyXalign, a freely available and easy-to-use software package to automatically correct for distortions in xCGE-LIF based glycan data. We demonstrate its ability to outperform conventional algorithms such as dynamic time warping and correlation optimized warping in terms of processing time and alignment accuracy for high-resolution datasets. Built upon a set of rapid algorithms, the tool includes an intuitive graphical user interface and allows full control over all parameters. Additionally, it visualizes the alignment process and enables the user to readjust misaligned results. Software and documentation are available at http://www.glyxera.com. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. 超限高层建筑电气设计重点简析%Analysis on Key Points of Electrical Design for Super High-rise Buildings

    Institute of Scientific and Technical Information of China (English)

    华锡锋

    2012-01-01

    In combination with project cases, the key points of electrical design for the following aspects of super high-rise buildings are deeply analyzed : power supply scheme, emergency / stand-by power source, transformation and distribution substation, and escape lighting etc.%结合工程案例.对超限高层建筑的供电方案、应急/备用电源、变配电所,以及疏散照明等电气设计重点内容作深入分析。

  15. The Power Control System of Constant Power Super-high Pressure Mercury Lamp%超高压汞灯功率控制研究

    Institute of Scientific and Technical Information of China (English)

    刘卫平; 葛劢冲

    2012-01-01

    A super-high pressure mercury lamp power of mask aligner is introduced in this paper,using analogue power feedback control,effectively improving power stability.The principle and major circuit structure of constant power control are emphatically expounded in this paper.%介绍了一种光刻机超高压汞灯电源,采用模拟功率反馈控制,有效提高了光源稳定性;重点阐述了恒功率控制的原理和主要电路结构。

  16. Effect of low-frequency electromagnetic field on the as-cast microstructure of a new super high strength aluminum alloy by horizontal continuous casting

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The super high strength aluminum alloy ingots with 100 mm in diameter were cast by the process of low-frequency electromagnetic horizontal continuous casting (LFEHC) and the effect of electromagnetic field on the as-cast microstructure was studied. Results show that microstructure of the sample prepared by the LFEHC process was greatly refined. Microstructures at the border and the center of the ingots were fine, uniform and rosette-shaped.Electromagnetic frequency plays a key role in microstructure refining. Fine and uniform microstructures can be obtained with optimal electromagnetic frequency. In this experiment, under a frequency of 30 Hz the microstructure was the finest and the most uniform.

  17. Technological development for super-high efficiency solar cells. Survey on the commercialization on analysis; Chokokoritsu taiyo denchi no gijutsu kaihatsu. Jitsuyoka kaiseki ni kansuru chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Tatsuta, M. [New Energy and Industrial Technology Development Organization, Tokyo (Japan)

    1994-12-01

    This paper reports the survey results on analysis of super-high efficiency solar cells for practical use in fiscal 1994. (1) On the survey on crystalline compound solar cells, it was pointed out that the present study target is III-V compound semiconductor solar cell, and efficiencies of 36-39% are theoretically expected by use of two-junction cells. (2) On structure of super-high efficiency solar cells of 40%, selection of upper and lower cell materials for multi-junction cells, high-efficiency tandem Si solar cells, and the merit and possibility of light collection operation were surveyed, and their issues were discussed. (3) On physical properties of mixed crystalline semiconductors and characteristic evaluation of solar cells, impurities, trap center, minority carrier life, and applicability of supper lattice structure to high-efficiency solar cells were surveyed. (4) On fabrication technology of compound semiconductor solar cells, various problems of and approaches to electrode formation and antireflection film technologies, the meaning and issues of thin film substrate technology and continuous process, trial calculation of costs, safety, and resource problem were surveyed.

  18. Simultaneous detection and quantitation of Chikungunya, dengue and West Nile viruses by multiplex RT-PCR assays and dengue virus typing using high resolution melting.

    Science.gov (United States)

    Naze, F; Le Roux, K; Schuffenecker, I; Zeller, H; Staikowsky, F; Grivard, P; Michault, A; Laurent, P

    2009-12-01

    Chikungunya (CHIKV), Dengue (DENV) and West Nile (WNV) viruses are arthropod-borne viruses that are able to emerge or re-emerge in many regions due to climatic changes and increase in travel. Since these viruses produce similar clinical signs it is important for physicians and epidemiologists to differentiate them rapidly. A molecular method was developed for their detection and quantitation in plasma samples and a DENV typing technique were developed. The method consisted in performing two multiplex real-time one-step RT-PCR assays, to detect and quantify the three viruses. Both assays were conducted in a single run, from a single RNA extract containing a unique coextracted and coamplified composite internal control. The quantitation results were close to the best detection thresholds obtained with simplex RT-PCR techniques. The differentiation of DENV types was performed using a High Resolution Melting technique. The assays enable the early diagnosis of the three arboviruses during viremia, including cases of coinfection. The method is rapid, specific and highly sensitive with a potential for clinical diagnosis and epidemiological surveillance. A DENV positive sample can be typed conveniently using the High Resolution Melting technique using the same apparatus.

  19. A Statistical study of the Doppler spectral width of high-latitude ionospheric F-region echoes recorded with SuperDARN coherent HF radars

    Directory of Open Access Journals (Sweden)

    J.-P. Villain

    Full Text Available The HF radars of the Super Dual Auroral Radar Network (SuperDARN provide measurements of the E × B drift of ionospheric plasma over extended regions of the high-latitude ionosphere. We have conducted a statistical study of the associated Doppler spectral width of ionospheric F-region echoes. The study has been conducted with all available radars from the Northern Hemisphere for 2 specific periods of time. Period 1 corresponds to the winter months of 1994, while period 2 covers October 1996 to March 1997. The distributions of data points and average spectral width are presented as a function of Magnetic Latitude and Magnetic Local Time. The databases are very consistent and exhibit the same features. The most stringent features are: a region of very high spectral width, collocated with the ionospheric LLBL/cusp/mantle region; an oval shaped region of high spectral width, whose equator-ward boundary matches the poleward limit of the Holzworth and Meng auroral oval. A simulation has been conducted to evaluate the geometrical and instrumental effects on the spectral width. It shows that these effects cannot account for the observed spectral features. It is then concluded that these specific spectral width characteristics are the signature of ionospheric/magnetospheric coupling phenomena.

    Key words. Ionosphere (auroral ionosphere; ionosphere-magnetosphere interactions; ionospheric irregularities

  20. Braking materials for emergency stop device of super high speed elevator (810 m/min); 810 m/min erebeta hijo tome sochiyo masatsuzai

    Energy Technology Data Exchange (ETDEWEB)

    Okada, R.; Yamada, T.; Sugahara, J. [Hitachi Ltd., Tokyo (Japan)

    1995-04-20

    Accompanied with a super multistoried building, making the elevator a higher speed is essential and it requires for a performance rise not only on drive unit and control unit, but also on safety device as well, as for especially an emergency stop device to get the cage, which has a kinetic energy proportional to the square of speed, stopped, its performance improvement is indispensable. Because it was anticipated that a braking would become difficult with a speed exceeding 800 m/min by using the iron system materials centering around cast iron used conventionally, an emergency stop device using the special ceramics as a friction material has been developed. In order to develop an elevator with a super high speed of 810 m/min this time, a development of the friction material for emergency stop device, which can brake stably the cage with a kinetic energy substantially exceeding the conventional value, has been advanced. As a result, a strength drop at a high temperature was prevented by adding Cr, Ni and P, and moreover a cast iron with 1,5 times in mean friction coefficient and about 1/10 in specific abrasive quantity compared with FC 250 was developed, and furthermore an emergency stop device with a high performance, which guarantees more than 3 times of braking energy in the emergency stop device of elevator with a speed of 540 m/min, was realized. 5 refs., 8 figs., 1 tab.

  1. SuperCam, a 64-pixel heterodyne imaging array for the 870 micron atmospheric window

    CERN Document Server

    Groppi, C; Kulesa, C; Pütz, P; Golish, D; Gensheimer, P; Hedden, A; Bussmann, S; Weinreb, S; Kuiper, T; Kooi, J; Jones, G; Bardin, J; Mani, H S; Lichtenberger, A; Narayanan, G; Groppi, Christopher; Walker, Christopher; Kulesa, Craig; Puetz, Patrick; Golish, Dathon; Gensheimer, Paul; Hedden, Abigail; Bussmann, Shane; Weinreb, Sander; Kuiper, Thomas; Kooi, Jacob; Jones, Glenn; Bardin, Joseph; Mani, Hamdi; Lichtenberger, Arthur; Narayanan, Gopal

    2006-01-01

    We report on the development of SuperCam, a 64 pixel, superheterodyne camera designed for operation in the astrophysically important 870 micron atmospheric window. SuperCam will be used to answer fundamental questions about the physics and chemistry of molecular clouds in the Galaxy and their direct relation to star and planet formation. The advent of such a system will provide an order of magnitude increase in mapping speed over what is now available and revolutionize how observational astronomy is performed in this important wavelength regime. Unlike the situation with bolometric detectors, heterodyne receiver systems are coherent, retaining information about both the amplitude and phase of the incident photon stream. From this information a high resolution spectrum of the incident light can be obtained without multiplexing. SuperCam will be constructed by stacking eight, 1x8 rows of fixed tuned, SIS mixers. The IF output of each mixer will be connected to a low-noise, broadband MMIC amplifier integrated in...

  2. Microsphere Super-resolution Imaging

    CERN Document Server

    Wang, Zengbo

    2015-01-01

    Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record resolution of 50 nm under white lights. Different samples have been directly imaged in high resolution and real time without labelling, including both non-biological (nano devices, structures and materials) and biological (subcellular details, viruses) samples. This chapter reviews the technique, which covers its background, fundamentals, experiments, mechanisms as well as the future outlook.

  3. Functional Multiplex PageRank

    CERN Document Server

    Iacovacci, Jacopo; Arenas, Alex; Bianconi, Ginestra

    2016-01-01

    Recently it has been recognized that many complex social, technological and biological networks have a multilayer nature and can be described by multiplex networks. Multiplex networks are formed by a set of nodes connected by links having different connotations forming the different layers of the multiplex. Characterizing the centrality of the nodes in a multiplex network is a challenging task since the centrality of the node naturally depends on the importance associated to links of a certain type. Here we propose to assign to each node of a multiplex network a centrality called Functional Multiplex PageRank that is a function of the weights given to every different pattern of connections (multilinks) existent in the multiplex network between any two nodes. Since multilinks distinguish all the possible ways in which the links in different layers can overlap, the Functional Multiplex PageRank can describe important non-linear effects when large relevance or small relevance is assigned to multilinks with overl...

  4. High-throughput droplet analysis and multiplex DNA detection in the microfluidic platform equipped with a robust sample-introduction technique.

    Science.gov (United States)

    Chen, Jinyang; Ji, Xinghu; He, Zhike

    2015-08-12

    In this work, a simple, flexible and low-cost sample-introduction technique was developed and integrated with droplet platform. The sample-introduction strategy was realized based on connecting the components of positive pressure input device, sample container and microfluidic chip through the tygon tubing with homemade polydimethylsiloxane (PDMS) adaptor, so the sample was delivered into the microchip from the sample container under the driving of positive pressure. This sample-introduction technique is so robust and compatible that could be integrated with T-junction, flow-focus or valve-assisted droplet microchips. By choosing the PDMS adaptor with proper dimension, the microchip could be flexibly equipped with various types of familiar sample containers, makes the sampling more straightforward without trivial sample transfer or loading. And the convenient sample changing was easily achieved by positioning the adaptor from one sample container to another. Benefiting from the proposed technique, the time-dependent concentration gradient was generated and applied for quantum dot (QD)-based fluorescence barcoding within droplet chip. High-throughput droplet screening was preliminarily demonstrated through the investigation of the quenching efficiency of ruthenium complex to the fluorescence of QD. More importantly, multiplex DNA assay was successfully carried out in the integrated system, which shows the practicability and potentials in high-throughput biosensing.

  5. A robust multi-shot scan strategy for high-resolution diffusion weighted MRI enabled by multiplexed sensitivity-encoding (MUSE).

    Science.gov (United States)

    Chen, Nan-Kuei; Guidon, Arnaud; Chang, Hing-Chiu; Song, Allen W

    2013-05-15

    Diffusion weighted magnetic resonance imaging (DWI) data have been mostly acquired with single-shot echo-planar imaging (EPI) to minimize motion induced artifacts. The spatial resolution, however, is inherently limited in single-shot EPI, even when the parallel imaging (usually at an acceleration factor of 2) is incorporated. Multi-shot acquisition strategies could potentially achieve higher spatial resolution and fidelity, but they are generally susceptible to motion-induced phase errors among excitations that are exacerbated by diffusion sensitizing gradients, rendering the reconstructed images unusable. It has been shown that shot-to-shot phase variations may be corrected using navigator echoes, but at the cost of imaging throughput. To address these challenges, a novel and robust multi-shot DWI technique, termed multiplexed sensitivity-encoding (MUSE), is developed here to reliably and inherently correct nonlinear shot-to-shot phase variations without the use of navigator echoes. The performance of the MUSE technique is confirmed experimentally in healthy adult volunteers on 3Tesla MRI systems. This newly developed technique should prove highly valuable for mapping brain structures and connectivities at high spatial resolution for neuroscience studies.

  6. Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations

    Energy Technology Data Exchange (ETDEWEB)

    Deng, Liulin; Webb, Ian K.; Garimella, Sandilya V. B.; Hamid, Ahmed M.; Zheng, Xueyun; Norheim, Randolph V.; Prost, Spencer A.; Anderson, Gordon A.; Sandoval, Jeremy A.; Baker, Erin S.; Ibrahim, Yehia M.; Smith, Richard D.

    2017-04-05

    Ion mobility (IM) separations have a broad range of analytical applications, but insufficient resolution limits many applications. Here we report on traveling wave (TW) ion mobility (IM) separations in a Serpentine Ultra-long Path with Extended Routing (SUPER) Structures for Lossless Ion Manipulations (SLIM) module in conjunction with mass spectrometry (MS). The extended routing utilized multiple passes was facilitated by the introduction of a lossless ion switch at the end of the ion path that either directed ions to the MS detector or to another pass through the serpentine separation region, providing theoretically unlimited TWIM path lengths. Ions were confined in the SLIM by rf fields in conjunction with a DC guard bias, enabling essentially lossless TW transmission over greatly extended paths (e.g., ~1094 meters over 81 passes through the 13.5 m serpentine path). In this multi-pass SUPER TWIM provided resolution approximately proportional to the square root of the number of passes (or path length). More than 30-fold higher IM resolution for Agilent tuning mix m/z 622 and 922 ions (~340 vs. ~10) was achieved for 40 passes compared to commercially available drift tube IM and other TWIM-based platforms. An initial evaluation of the isomeric sugars Lacto-N-hexaose and Lacto-N-neohexaose showed the isomeric structures to be baseline resolved, and a new conformational feature for Lacto-N-neohexaose was revealed after 9 passes. The new SLIM SUPER high resolution TWIM platform has broad utility in conjunction with MS and is expected to enable a broad range of previously challenging or intractable separations.

  7. Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations.

    Science.gov (United States)

    Deng, Liulin; Webb, Ian K; Garimella, Sandilya V B; Hamid, Ahmed M; Zheng, Xueyun; Norheim, Randolph V; Prost, Spencer A; Anderson, Gordon A; Sandoval, Jeremy A; Baker, Erin S; Ibrahim, Yehia M; Smith, Richard D

    2017-04-18

    Ion mobility (IM) separations have a broad range of analytical applications, but insufficient resolution often limits their utility. Here, we report on ion mobility separations in a structures for lossless ion manipulations (SLIM) serpentine ultralong path with extended routing (SUPER) traveling wave (TW) ion mobility (IM) module in conjunction with mass spectrometry (MS). Ions were confined in the SLIM by rf fields in conjunction with a DC guard bias, enabling essentially lossless TW transmission over greatly extended paths. The extended routing utilized multiple passes (e.g., ∼1094 m over 81 passes through the 13.5 m serpentine path) and was facilitated by the introduction of a lossless ion switch that allowed ions to be directed to either the MS detector or for another pass through the serpentine separation region, allowing theoretically unlimited IM path lengths. The multipass SUPER IM-MS provided resolution approximately proportional to the square root of the number of passes (or total path length). More than 30-fold higher IM resolution (∼340 vs ∼10) for Agilent tuning mix m/z 622 and 922 ions was achieved for 40 passes compared to commercially available drift tube IM and other TWIM-based platforms. An initial evaluation of the isomeric sugars lacto-N-hexaose and lacto-N-neohexaose showed the isomeric structures to be baseline resolved, and a new conformational feature for lacto-N-neohexaose was revealed after 9 passes. The new SLIM SUPER high resolution TWIM platform has broad utility in conjunction with MS and is expected to enable a broad range of previously challenging or intractable separations.

  8. Spatial code division multiplexing in optical data processing

    Science.gov (United States)

    Zalevsky, Zeev; Mendlovic, David; Solomon, Jonathan

    2002-09-01

    The increasing popularity of optical communication has also brought a demand for broader bandwidth. The natural trend was to implement methods from traditional communication on optical fibers. One of the most effective "traditional" methods is code division multiple access (CDMA). In this paper, we suggest the use of this approach for spatial coding applied to images, and for superresolution. In spatial coding, the approach is to multiplex several filters into one plane while keeping their mutual orthogonality. It is shown that within specific restrictions the output of all the filters can be sampled in the original image resolution and fully recovered through an all-optical setup. A theoretical analysis of such a setup is brought as well as experimental demonstrations are presented. Implementation for invariant pattern recognition is suggested. In superresolution, we propose using code division multiplexing in order to transfer a wider range of frequencies. Traditional methods for super resolution have sacrificed field of view for resolution. These methods multiplexed the signal on different carriers. Code division multiplexing for such a usage, has been shown to have superior capabilities, nearing almost Shannon's channel capacity limit. To enable such multiplexing we propose a unique setup that creates an incoherent cosine transform of the image. A theoretical analysis of the setup is brought and later compared with the empirical results.

  9. "Stereoscopic" 2D super-microporous phosphazene-based covalent organic framework: Design, synthesis and selective sorption towards uranium at high acidic condition.

    Science.gov (United States)

    Zhang, Shuang; Zhao, Xiaosheng; Li, Bo; Bai, Chiyao; Li, Yang; Wang, Lei; Wen, Rui; Zhang, Meicheng; Ma, Lijian; Li, Shoujian

    2016-08-15

    So far, only five primary elements (C, H, O, N and B) and two types of spatial configuration (C2-C4, C6 and Td) are reported to build the monomers for synthesis of covalent organic frameworks (COFs), which have partially limited the route selection for accessing COFs with new topological structure and novel properties. Here, we reported the design and synthesis of a new "stereoscopic" 2D super-microporous phosphazene-based covalent organic framework (MPCOF) by using hexachorocyclotriphosphazene (a P-containing monomer in a C3-like spatial configuration) and p-phenylenediamine (a linker). The as-synthesized MPCOF shows high crystallinity, relatively high heat and acid stability and distinctive super-microporous structure with narrow pore-size distributions ranging from 1.0-2.1nm. The results of batch sorption experiments with a multi-ion solution containing 12 co-existing cations show that in the pH range of 1-2.5, MPCOF exhibits excellent separation efficiency for uranium with adsorption capacity more than 71mg/g and selectivity up to record-breaking 92%, and furthermore, an unreported sorption capacity (>50mg/g) and selectivity (>60%) were obtained under strong acidic condition (1M HNO3). Studies on sorption mechanism indicate that the uranium separation by MPCOF in acidic solution is realized mainly through both intra-particle diffusion and size-sieving effect.

  10. Mesoporous Li4Ti5O12 nanoclusters anchored on super-aligned carbon nanotubes as high performance electrodes for lithium ion batteries

    Science.gov (United States)

    Sun, Li; Kong, Weibang; Wu, Hengcai; Wu, Yang; Wang, Datao; Zhao, Fei; Jiang, Kaili; Li, Qunqing; Wang, Jiaping; Fan, Shoushan

    2015-12-01

    Mesoporous lithium titanate (LTO) nanoclusters are in situ synthesized in a network of super aligned carbon nanotubes (SACNTs) via a solution-based method followed by heat treatment in air. In the LTO-CNT composite, SACNTs not only serve as the skeleton to support a binder-free electrode, but also render the composite with high conductivity, flexibility, and mechanical strength. The homogeneously dispersed LTO nanoclusters among the SACNTs allow each LTO grain to effectively access the electrolyte and the conductive network, benefiting both ion and electron transport. By the incorporation of LTO into the CNT network, mechanical reinforcement is also achieved. When serving as a negative electrode for lithium ion batteries, such a robust composite-network architecture provides the electrodes with effective charge transport and structural integrity, leading to high-performance flexible electrodes with high capacity, high rate capability, and excellent cycling stability.Mesoporous lithium titanate (LTO) nanoclusters are in situ synthesized in a network of super aligned carbon nanotubes (SACNTs) via a solution-based method followed by heat treatment in air. In the LTO-CNT composite, SACNTs not only serve as the skeleton to support a binder-free electrode, but also render the composite with high conductivity, flexibility, and mechanical strength. The homogeneously dispersed LTO nanoclusters among the SACNTs allow each LTO grain to effectively access the electrolyte and the conductive network, benefiting both ion and electron transport. By the incorporation of LTO into the CNT network, mechanical reinforcement is also achieved. When serving as a negative electrode for lithium ion batteries, such a robust composite-network architecture provides the electrodes with effective charge transport and structural integrity, leading to high-performance flexible electrodes with high capacity, high rate capability, and excellent cycling stability. Electronic supplementary information

  11. Super Rice Breeding in China

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@I. Demand for super high yield rice in China   Rice is one of the main staple food in China. The performance of rice sector in production and yield had been very impressive in the last four decades. However, rice production and yield has stagnated since 1990.

  12. HLA-DRB1, -DRB3, -DRB4 and -DRB5 genotyping at a super-high resolution level by long range PCR and high-throughput sequencing.

    Science.gov (United States)

    Ozaki, Y; Suzuki, S; Shigenari, A; Okudaira, Y; Kikkawa, E; Oka, A; Ota, M; Mitsunaga, S; Kulski, J K; Inoko, H; Shiina, T

    2014-01-01

    Super high-resolution single molecule sequence-based typing (SS-SBT) is a human leukocyte antigen (HLA) DNA typing method to the field 4 level of allelic resolution (formerly known as eight-digit typing) to efficiently detect new and null alleles without phase ambiguity by combination of long ranged polymerase chain reaction (PCR) amplification and next-generation sequencing (NGS) technologies. We previously reported the development and application of the SS-SBT method for the eight classical HLA loci, A, B, C, DRB1, DQA1, DQB1, DPA1 and DPB1. In this article, we describe the development of the SS-SBT method for three DRB1 linked loci, DRB3, DRB4 and DRB5 (DRB3/4/5) and characterization of DRB1-DRB3/4/5 haplotype structures to the field 4 level. Locus specific PCR primers for DRB3/4/5 were designed to amplify the gene regions from intron 1 to exon 6 [3' untranslated region (3'UTR)]. In total 20 DRB1 and 13 DRB3/4/5 allele sequences were determined by the SS-SBT to the field 4 level without phase ambiguity using 19 DR51, DR52 and DR53 positive genomic DNA samples obtained from Japanese. Moreover, 18 DRB1-DRB3/4/5 haplotypes were estimated to the field 4 level by the SS-SBT method in contrast to 10 haplotypes estimated by conventional methods to the field 1 level (formerly known as two digit typing). Therefore, DRB1-DRB3/4/5 haplotyping by SS-SBT is expected to provide informative data for improved HLA matching in medical research, transplantation procedures, HLA-related disease studies and human population diversity studies.

  13. High-efficiency multiplex capillary electrophoresis single strand conformation polymorphism (multi-CE-SSCP) mutation screening of SCN5A: a rapid genetic approach to cardiac arrhythmia.

    Science.gov (United States)

    Hofman-Bang, J; Behr, E R; Hedley, P; Tfelt-Hansen, J; Kanters, J K; Haunsøe, S; McKenna, W J; Christiansen, M

    2006-06-01

    Mutations in the SCN5A gene coding for the alpha-subunit of the cardiac Na(+) ion channel cause long QT syndrome, Brugada syndrome, idiopathic ventricular fibrillation, sick sinus node syndrome, progressive conduction disease, dilated cardiomyopathy and atrial standstill. These diseases exhibit variable expressivity, and identification of gene carriers is clinically important, particularly in sudden infant and adult death syndromes. The SCN5A gene comprises 28 exons distributed over 100 kbp of genomic sequence at chromosome 3p21. Disease-causing mutations are private and scattered over the DNA sequence, making it difficult to screen for specific mutations. We developed a multiplex capillary-electrophoresis single-strand conformation polymorphism (Multi-CE-SSCP) mutation screening protocol on the ABI 3100 platform and applied it to 10 previously slab-gel SSCP identified mutations and SNPs and used it to identify one novel deletion. The method is highly efficient, with a turnover of 23 patients per 24 h and a false positive rate of 0.5% of the analyzed amplicons. Each variant has a particular elution pattern, and all 20 carriers of the H558R polymorphism out of 57 persons were correctly identified. We suggest that the method could become part of routine work-up of patients with suspicious syncope and of members of families with sudden unexplained death.

  14. Super resolution WiFi indoor localization and tracking

    OpenAIRE

    Salman, N.; Alsindi, N; Mihaylova, L.; Kemp, AH

    2014-01-01

    In this paper, we present a complete framework for accurate indoor positioning and tracking using the 802.11a WiFi network. Channel frequency response is first estimated via the least squares (LS) method using an orthogonal frequency division multiplexing (OFDM) pilot symbol. For accurate time of arrival (ToA) distance estimates in multipath environments, super resolution technique i.e. Multiple Signal Classification (MUSIC) is used which capitalizes on the autocorrelation matrix of the estim...

  15. Multiplexing spheroid volume, resazurin and acid phosphatase viability assays for high-throughput screening of tumour spheroids and stem cell neurospheres.

    Science.gov (United States)

    Ivanov, Delyan P; Parker, Terry L; Walker, David A; Alexander, Cameron; Ashford, Marianne B; Gellert, Paul R; Garnett, Martin C

    2014-01-01

    Three-dimensional cell culture has many advantages over monolayer cultures, and spheroids have been hailed as the best current representation of small avascular tumours in vitro. However their adoption in regular screening programs has been hindered by uneven culture growth, poor reproducibility and lack of high-throughput analysis methods for 3D. The objective of this study was to develop a method for a quick and reliable anticancer drug screen in 3D for tumour and human foetal brain tissue in order to investigate drug effectiveness and selective cytotoxic effects. Commercially available ultra-low attachment 96-well round-bottom plates were employed to culture spheroids in a rapid, reproducible manner amenable to automation. A set of three mechanistically different methods for spheroid health assessment (Spheroid volume, metabolic activity and acid phosphatase enzyme activity) were validated against cell numbers in healthy and drug-treated spheroids. An automated open-source ImageJ macro was developed to enable high-throughput volume measurements. Although spheroid volume determination was superior to the other assays, multiplexing it with resazurin reduction and phosphatase activity produced a richer picture of spheroid condition. The ability to distinguish between effects on malignant and the proliferating component of normal brain was tested using etoposide on UW228-3 medulloblastoma cell line and human neural stem cells. At levels below 10 µM etoposide exhibited higher toxicity towards proliferating stem cells, whereas at concentrations above 10 µM the tumour spheroids were affected to a greater extent. The high-throughput assay procedures use ready-made plates, open-source software and are compatible with standard plate readers, therefore offering high predictive power with substantial savings in time and money.

  16. Multiplexing spheroid volume, resazurin and acid phosphatase viability assays for high-throughput screening of tumour spheroids and stem cell neurospheres.

    Directory of Open Access Journals (Sweden)

    Delyan P Ivanov

    Full Text Available Three-dimensional cell culture has many advantages over monolayer cultures, and spheroids have been hailed as the best current representation of small avascular tumours in vitro. However their adoption in regular screening programs has been hindered by uneven culture growth, poor reproducibility and lack of high-throughput analysis methods for 3D. The objective of this study was to develop a method for a quick and reliable anticancer drug screen in 3D for tumour and human foetal brain tissue in order to investigate drug effectiveness and selective cytotoxic effects. Commercially available ultra-low attachment 96-well round-bottom plates were employed to culture spheroids in a rapid, reproducible manner amenable to automation. A set of three mechanistically different methods for spheroid health assessment (Spheroid volume, metabolic activity and acid phosphatase enzyme activity were validated against cell numbers in healthy and drug-treated spheroids. An automated open-source ImageJ macro was developed to enable high-throughput volume measurements. Although spheroid volume determination was superior to the other assays, multiplexing it with resazurin reduction and phosphatase activity produced a richer picture of spheroid condition. The ability to distinguish between effects on malignant and the proliferating component of normal brain was tested using etoposide on UW228-3 medulloblastoma cell line and human neural stem cells. At levels below 10 µM etoposide exhibited higher toxicity towards proliferating stem cells, whereas at concentrations above 10 µM the tumour spheroids were affected to a greater extent. The high-throughput assay procedures use ready-made plates, open-source software and are compatible with standard plate readers, therefore offering high predictive power with substantial savings in time and money.

  17. Multiplex editing system

    DEFF Research Database (Denmark)

    2015-01-01

    The present invention relates to a multiplex editing system. The system allows multiple editing of nucleic acid sequences such as genomic sequences, such as knockins of genes of interest in a genome, knockouts of genomic sequences and/or allele replacement. Also provided herein are a method...... for editing nucleic acids and a cell comprising a stably integrated endonuclease....

  18. Multiplex PageRank

    CERN Document Server

    Halu, Arda; Pansaraza, Pietro; Bianconi, Ginestra

    2013-01-01

    Many complex systems can be described as multiplex networks in which the same nodes can interact with one another in different layers, thus forming a set of interacting and co-evolving networks. Examples of such multiplex systems are social networks where people are involved in different types of relationships and interact through various forms of communication media. The ranking of nodes in multiplex networks is one of the most pressing and challenging tasks that research on complex networks is currently facing. When pairs of nodes can be connected through multiple links and in multiple layers, the ranking of nodes should necessarily reflect the importance of nodes in one layer as well as their importance in other interdependent layers. In this paper, we draw on the idea of biased random walks to define the Multiplex PageRank centrality measure in which the effects of the interplay between networks on the centrality of nodes are directly taken into account. In particular, depending on the intensity of the in...

  19. Arthrogryposis multiplex congenita

    DEFF Research Database (Denmark)

    Linnet, Karen Markussen; Balslev, Thomas; Møller-Madsen, Bjarne

    2015-01-01

    Arthrogryposis multiplex congenita (AMC) is a sign rather than a diagnosis. It implies contractures in multiple body areas and occurs in 1:3,000-5,000 live births. Primary aetiologies include neuropathic, myopathic, metabolic, end plate and vascular disorder affecting the developing foetus...

  20. Wired and wireless convergent extended-reach optical access network using direct-detection of all-optical OFDM super-channel signal.

    Science.gov (United States)

    Chow, C W; Yeh, C H; Sung, J Y; Hsu, C W

    2014-12-15

    We propose and demonstrate the feasibility of using all-optical orthogonal frequency division multiplexing (AO-OFDM) for the convergent optical wired and wireless access networks. AO-OFDM relies on all-optically generated orthogonal subcarriers; hence, high data rate (> 100 Gb/s) can be easily achieved without hitting the speed limit of electronic digital-to-analog and analog-to-digital converters (DAC/ADC). A proof-of-concept convergent access network using AO-OFDM super-channel (SC) is demonstrated supporting 40 - 100 Gb/s wired and gigabit/s 100 GHz millimeter-wave (MMW) ROF transmissions.

  1. The Microwave SQUID Multiplexer

    Science.gov (United States)

    Mates, John Arthur Benson

    2011-12-01

    This thesis describes a multiplexer of Superconducting Quantum Interference Devices (SQUIDs) with low-noise, ultra-low power dissipation, and great scalability. The multiplexer circuit measures the magnetic flux in a large number of unshunted rf SQUIDs by coupling each SQUID to a superconducting microwave resonator tuned to a unique resonance frequency and driving the resonators from a common feedline. A superposition of microwave tones measures each SQUID simultaneously using only two coaxial cables between the cryogenic device and room temperature. This multiplexer will enable the instrumentation of arrays with hundreds of thousands of low-temperature detectors for new applications in cosmology, materials analysis, and nuclear non-proliferation. The driving application of the Microwave SQUID Multiplexer is the readout of large arrays of superconducting transition-edge sensors, by some figures of merit the most sensitive detectors of electromagnetic signals over a span of more than nine orders of magnitude in energy, from 40 GHz microwaves to 200 keV gamma rays. Modern transition-edge sensors have noise-equivalent power as low as 10-20 W / Hz1/2 and energy resolution as good as 2 eV at 6 keV. These per-pixel sensitivities approach theoretical limits set by the underlying signals, motivating a rapid increase in pixel count to access new science. Compelling applications, like the non-destructive assay of nuclear material for treaty verification or the search for primordial gravity waves from inflation use arrays of these detectors to increase collection area or tile a focal plane. We developed three generations of SQUID multiplexers, optimizing the first for flux noise 0.17 muPhi0 / Hz1/2, the second for input current noise 19 pA / Hz1/2, and the last for practical multiplexing of large arrays of cosmic microwave background polarimeters based on transition-edge sensors. Using the last design we demonstrated multiplexed readout of prototype polarimeters with the

  2. The analysis of high explosives by liquid chromatography/electrospray ionization mass spectrometry: multiplexed detection of negative ion adducts.

    Science.gov (United States)

    Mathis, John A; McCord, Bruce R

    2005-01-01

    The negative ion electrospray ionization mass spectrometric (ESI-MS) detection of adducts of high explosives with chloride, formate, acetate, and nitrate was used to demonstrate the gas-phase interaction of neutral explosives with these anions. The relative intensities of the adduct species were determined to compare the competitive formation of the selected high explosives and anions. The relative stability of the adduct species varies, yielding preferential formation of certain anionic adducts with different high explosives. To exploit this effect, an isocratic high-performance liquid chromatography (HPLC)/ESI-MS method was developed and used for the simultaneous analysis of high explosives using two different techniques for the addition of the anionic additives; pre- and post-column. The results show that the pre-column approach provides similar results with improved selectivity for specific explosives. By detecting characteristic adduct species for each explosive, this method provides a qualitative and quantitative approach for the analysis and identification of high explosives.

  3. Effect of High Efficient Super Plasticizer on Performance of Iron Tailing High-quality Concrete%高效减水剂对铁尾矿高性能混凝土性能的影响

    Institute of Scientific and Technical Information of China (English)

    崔兴兰; 倪文; 刘倩

    2011-01-01

    将北京密云铁尾矿与高炉水淬矿渣、水泥熟料、脱硫石膏通过三段混合磨矿形成胶凝材料,然后将胶凝材料与铁尾矿混合,并加入高效减水剂,制备成高性能混凝土材料,其中铁尾矿总掺量达到70%.固定其它参数与试验条件,改变高效减水剂的种类和掺量,研究高效减水剂对铁尾矿高性能混凝土性能的影响.试验结果表明:聚羧酸系高效减水剂对新拌混凝土的工作性能最有利,掺入UNF一5的硬化混凝土各龄期的抗压强度最高,28 d强度已经达到80 MPa.%The cementitious materials was formed by three steps milling of Miyun iron ore tailings with water quenched high furnace slag, cement clinker and desulfurized gypsum, and then was mixed with iron tailings to prepare high performance concrete by adding high efficient super plasticizer, in which the total content of iron tailings was 70% by weight. The effect of high efficient super plasticizer on the concrete samples was studied by varying the types and dosage of super plasticizers when the experimental conditions and other parameters were fixed. The results showed that polycarboxylate-based super plasticizer has good effects on the workability of fresh concrete. The hardened concrete samples blended with UNF-5 own highest compressive strength at each stage and reach 80 MPa at 28 d.

  4. On comparison of schemes for air-conditioners in some super-high-rise office buildings%某超高层办公楼空调方案比选

    Institute of Scientific and Technical Information of China (English)

    杨帆; 张博

    2012-01-01

    Under the environment of prosperous development of super-high-rise buildings,the paper analyzes some problems in the design of the air-conditioner system in the super-high-rise buildings according to the features of the super-high-rise buildings and combining some practice experience,compares many schemes,and chooses the most economical and applied air-conditioner system.%在超高层建筑蓬勃发展的环境下,针对超高层建筑特点,结合实践经验,分析了超高层空调系统设计中需注意的问题,并对多种方案进行比选,进而选出最经济、最适用的空调系统。

  5. Super insulating aerogel glazing

    DEFF Research Database (Denmark)

    Schultz, Jørgen Munthe; Jensen, Karsten Ingerslev; Kristiansen, Finn Harken

    2004-01-01

    Monolithic silica aerogel offers the possibility of combining super insulation and high solar energy transmittance, which has been the background for a previous and a current EU project on research and development of monolithic silica aerogel as transparent insulation in windows. Generally, windows...... form the weakest part of the thermal envelope with respect to heat loss coefficient, but on the other hand also play an important role for passive solar energy utilisation. For window orientations other than south, the net energy balance will be close to or below zero. However, the properties...... of aerogel glazing will allow for a positive net energy gain even for north facing vertical windows in a Danish climate during the heating season. This means that high quality daylight can be obtained even with additional energy gain. On behalf of the partners of the two EU projects, results related...

  6. Yield and Water Productivity Responses to Irrigation Cut-off Strategies after Fruit Set Using Stem Water Potential Thresholds in a Super-High Density Olive Orchard.

    Science.gov (United States)

    Ahumada-Orellana, Luis E; Ortega-Farías, Samuel; Searles, Peter S; Retamales, Jorge B

    2017-01-01

    An increase in the land area dedicated to super-high density olive orchards has occurred in Chile in recent years. Such modern orchards have high irrigation requirements, and optimizing water use is a priority. Moreover, this region presents low water availability, which makes necessary to establish irrigation strategies to improve water productivity. An experiment was conducted during four consecutive growing seasons (2010-2011 to 2013-2014) to evaluate the responses of yield and water productivity to irrigation cut-off strategies. These strategies were applied after fruit set using midday stem water potential (Ψstem) thresholds in a super-high density olive orchard (cv. Arbequina), located in the Pencahue Valley, Maule Region, Chile. The experimental design was completely randomized with four irrigation cut-off treatments based on the Ψstem thresholds and four replicate plots per treatment (five trees per plot). Similar to commercial growing conditions in our region, the Ψstem in the T1 treatment was maintained between -1.4 and -2.2 MPa (100% of actual evapotranspiration), while T2, T3 and T4 treatments did not receive irrigation from fruit set until they reached a Ψstem threshold of approximately -3.5, -5.0, and -6.0 MPa, respectively. Once the specific thresholds were reached, irrigation was restored and maintained as T1 in all treatments until fruits were harvested. Yield and its components were not significantly different between T1 and T2, but fruit yield and total oil yield, fruit weight, and fruit diameter were decreased by the T3 and T4 treatments. Moreover, yield showed a linear response with water stress integral (SΨ), which was strongly influenced by fruit load. Total oil content (%) and pulp/stone ratio were not affected by the different irrigation strategies. Also, fruit and oil water productivities were significantly greater in T1 and T2 than in the T3 and T4. Moreover, the T2, T3, and T4 treatments averaged 37, 51, and 72 days without

  7. Extracting information from multiplex networks.

    Science.gov (United States)

    Iacovacci, Jacopo; Bianconi, Ginestra

    2016-06-01

    Multiplex networks are generalized network structures that are able to describe networks in which the same set of nodes are connected by links that have different connotations. Multiplex networks are ubiquitous since they describe social, financial, engineering, and biological networks as well. Extending our ability to analyze complex networks to multiplex network structures increases greatly the level of information that is possible to extract from big data. For these reasons, characterizing the centrality of nodes in multiplex networks and finding new ways to solve challenging inference problems defined on multiplex networks are fundamental questions of network science. In this paper, we discuss the relevance of the Multiplex PageRank algorithm for measuring the centrality of nodes in multilayer networks and we characterize the utility of the recently introduced indicator function Θ̃(S) for describing their mesoscale organization and community structure. As working examples for studying these measures, we consider three multiplex network datasets coming for social science.

  8. Extracting information from multiplex networks

    Science.gov (United States)

    Iacovacci, Jacopo; Bianconi, Ginestra

    2016-06-01

    Multiplex networks are generalized network structures that are able to describe networks in which the same set of nodes are connected by links that have different connotations. Multiplex networks are ubiquitous since they describe social, financial, engineering, and biological networks as well. Extending our ability to analyze complex networks to multiplex network structures increases greatly the level of information that is possible to extract from big data. For these reasons, characterizing the centrality of nodes in multiplex networks and finding new ways to solve challenging inference problems defined on multiplex networks are fundamental questions of network science. In this paper, we discuss the relevance of the Multiplex PageRank algorithm for measuring the centrality of nodes in multilayer networks and we characterize the utility of the recently introduced indicator function Θ ˜ S for describing their mesoscale organization and community structure. As working examples for studying these measures, we consider three multiplex network datasets coming for social science.

  9. Calculus super review

    CERN Document Server

    2012-01-01

    Get all you need to know with Super Reviews! Each Super Review is packed with in-depth, student-friendly topic reviews that fully explain everything about the subject. The Calculus I Super Review includes a review of functions, limits, basic derivatives, the definite integral, combinations, and permutations. Take the Super Review quizzes to see how much you've learned - and where you need more study. Makes an excellent study aid and textbook companion. Great for self-study!DETAILS- From cover to cover, each in-depth topic review is easy-to-follow and easy-to-grasp - Perfect when preparing for

  10. Algebra & trigonometry super review

    CERN Document Server

    2012-01-01

    Get all you need to know with Super Reviews! Each Super Review is packed with in-depth, student-friendly topic reviews that fully explain everything about the subject. The Algebra and Trigonometry Super Review includes sets and set operations, number systems and fundamental algebraic laws and operations, exponents and radicals, polynomials and rational expressions, equations, linear equations and systems of linear equations, inequalities, relations and functions, quadratic equations, equations of higher order, ratios, proportions, and variations. Take the Super Review quizzes to see how much y

  11. Validation of a High-Throughput Multiplex Genetic Detection System for Helicobacter pylori Identification, Quantification, Virulence, and Resistance Analysis

    OpenAIRE

    Zhang, Yanmei; Zhao, Fuju; Kong, Mimi; Wang, Shiwen; Nan, Li; Hu, Binjie; Olszewski, Michal A.; Miao, Yingxin; Ji, Danian; Jiang, Wenrong; Fang, Yi; Zhang, Jinghao; Chen, Fei; Xiang, Ping; Wu, Yong

    2016-01-01

    Helicobacter pylori (H. pylori) infection is closely related to various gastroduodenal diseases. Virulence factors and bacterial load of H. pylori are associated with clinical outcomes, and drug-resistance severely impacts the clinical efficacy of eradication treatment. Existing detection methods are low-throughput, time-consuming and labor intensive. Therefore, a rapid and high-throughput method is needed for clinical diagnosis, treatment, and monitoring for H. pylori. High-throughput Multip...

  12. Sequential amplitude divided angular multiplexing encoding optical system design for high power excimer laser system%连续分振幅式高功率准分子激光角多路编码光路设计

    Institute of Scientific and Technical Information of China (English)

    胡云; 王大辉; 赵学庆

    2016-01-01

    In high power excimer laser system, angular multiplexing technique is employed to achieve both high energy and narrow pulse output. In this article, angular multiplexing technique was introduced, and a multiplexing encoding method was presented. This method encoded seed beam in two steps by sequential amplitude splitting. The optical elements were arranged in rectangle arrays and piled by layers. A specific optical design was made for XeCl high power excimer laser system in this laboratory. This method of angular multiplexing encoding has advantages of compacted space, small encoding error, good compatibility with alignment and measurement, and is also easy to fabricate and assemble. This design is adopted in the system and performs well.%在高功率准分子激光系统中,一般采用光学角多路技术来获得高能量窄脉冲输出。文中介绍了角多路技术原理,提出了一种采用矩形阵列和空间层叠光路结构的连续分振幅两次编码方式,并针对该实验室的XeCl高功率准分子激光系统进行了具体的编码光路设计,给出了设计实例。该方法具有编码结构紧凑,编码精度高,与光路准直、激光参数测量系统等兼容性好,便于加工制作和安装调节等优点,目前已在系统中应用,效果良好。

  13. High-pressure single-crystal X-ray diffraction study on Super Fluorous Phase B up to 7.4 GPa

    Science.gov (United States)

    Kuribayashi, T.; Kojitani, H.; Nagase, T.; Akaogi, M.; Mao, H.; Hemley, R.; Kudoh, Y.

    2008-12-01

    Super hydrous phase B (SUP-B), Mg10Si3O14(OH)4, is known as one of dense hydrous magnesium silicate minerals (DHMS). Also, super fluorous phase B (sup-B-F), Mg10Si3O14F4, was firstly synthesized by Hazen et al. (1997). In the crystal structure of SUP-B-F, F replaces OH in sup-B structure. Therefore, differences of behavior between SUP-B-F and SUP-B under high-pressure conditions directory reflect the effect of hydrogen bonding. In this study, to clarify the behavior of SUP-B-F under high-pressure condition, SUP-B-F was synthesized at 21 GPa and 1400°C conditions using the Kawai type multi anvil apparatus installed in Gakushu-in University. Using a synthesized single-crystal (0.04 × 0.04 × 0.03 mm3), high-pressure single-crystal X-ray diffraction measurements were conducted up to 7.4 GPa with synchrotron radiation. Lattice constants were determined at several pressure points up to 7.4 GPa. The isothermal bulk modulus of SUP-B-F, calculated from the Birch-Murnaghan Equation of State, was 154(2) GPa with a pressure derivative K'=4 (assuming). This value is significantly larger than that of super hydrous phase B (142.8(5) GPa with K"f=5.7 by Crichton et al., 1999). The X-ray reflection intensity data set was collected at 5.0 GPa using the four-circle diffractometer installed at the beam line BL-10A in Photon Factory, Japan. 198 reflections were used for structure refinement (the number of measured reflections is 786). After Lorentz- polarization correction and background reduction were applied for all intensity data, the crystallographic equivalent reflections (Orthorhombic, Pnnm) were averaged. Structure refinement results were R=5.0% and wR2=13.1%, respectively.

  14. 超高层建筑智能化系统若干问题浅析%Analysis on Some Problems about Intelligent Systems in Super High-rise Buildings

    Institute of Scientific and Technical Information of China (English)

    李志涛; 兰海民; 岳远波

    2011-01-01

    结合超高层建筑功能特点和智能化技术应用现状,提出超高层智能化设计要点,旨在探讨和提供适宜此类建筑特点的技术措施。%Combined with the function features and the present situation of intelligent technology in super high-rise buildings, this paper advances the design points of super high-rise buildings, which are aimed at discuss and provide technical measures that suitab

  15. Depth of focus increase by multiplexing programmable diffractive lenses.

    Science.gov (United States)

    Iemmi, C; Campos, J; Escalera, J C; López-Coronado, O; Gimeno, R; Yzuel, M J

    2006-10-30

    A combination of several diffractive lenses written onto a single programmable liquid crystal display (LCD) is proposed for increasing the Depth of Focus (DOF) of the imaging system as a whole. The lenses are spatially multiplexed in a random scheme onto the LCD. The axial irradiance distribution produced by each lens overlaps with the next one producing an extended focal depth. To compare the image quality of the multiplexed lenses, the Modulation Transfer Function (MTF) is calculated. Finally we obtain the experimental Point Spread Functions (PSF) for these multiplexed lenses and experimental results in which an extended object is illuminated under spatially incoherent monochromatic light. We compare the images obtained in the focal plane and in some defocused planes with the single lens and with three multiplexed lenses. The experimental results confirm that the multiplexed lenses produce a high increase in the depth of focus.

  16. Two-stage laparoscopic biliopancreatic diversion with duodenal switch as treatment of high-risk super-obese patients: analysis of complications.

    Science.gov (United States)

    Silecchia, G; Rizzello, M; Casella, G; Fioriti, M; Soricelli, E; Basso, N

    2009-05-01

    The aim of this study is to retrospectively analyze the incidence of complications after two-stage laparoscopic biliopancreatic diversion with duodenal switch (Lap BPD-DS) in high-risk super-obese patients and explore the possible predictive factors of specific complications after laparoscopic sleeve gastrectomy (SG). High-risk patients--body mass index (BMI) > 50 kg/m(2) with at least two major comorbidities: type 2 diabetes, obstructive sleep apnea syndrome (OSAS), hypertension--undergoing two-stage laparoscopic BPD-DS were retrospectively analysed. The SG pouch volume was 100-150 ml; in the second stage, the common channel and the alimentary loop were 100 cm and 150 cm, respectively. Eighty-seven patients (50 female, 57.5%) underwent SG (two open). The mean age was 41.8 +/- 10.22 years with BMI of 55.2 +/- 6.69 kg/m(2). Four patients had Prader-Willy syndrome. Fourteen (16.46%) patients (6 female, 42.8%) had postoperative complications such as bleeding, fistula, pulmonary embolism, transitory acute renal failure, and abdominal abscess. One patient died at postoperative day 5 of pulmonary embolism. One patient was reoperated for hemoperitoneum by laparoscopy. The risk of complications after SG was lower in patients where reinforcement of the suture line was used (0.492), while it was higher in men (1.780). Neither difference was statistically significant [p = not significant (NS)]. After 9-24 months, 27 patients (BMI 43 +/- 8 kg/m(2)) underwent a second stage of BPD-DS (two open). Major postoperative complications were registered in eight patients (29.6%): three bleeding, four duodeno-ileal stenosis and one rhabdomyolysis. Two cases of internal hernia required laparoscopic reoperation. The reoperation rate was 1/85 (1.2%) after SG and 2/27 (7.4%) after second stage. Complications after SG greatly decrease after the learning curve period and can be successfully managed without need of reoperation. Suture-line reinforcement, at least selectively in the middle

  17. Multiplex and quantitative pathogen detection with high-resolution capillary electrophoresis-based single-strand conformation polymorphism.

    Science.gov (United States)

    Hwang, Hee Sung; Shin, Gi Won; Chung, Boram; Na, Jeongkyeong; Jung, Gyoo Yeol

    2013-01-01

    Among the molecular diagnostic methods for bacteria-induced diseases, capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP) combined with 16S rRNA gene-specific PCR has enormous potential because it can separate sequence variants using a simple procedure. However, conventional CE-SSCP systems have limited resolution and cannot separate most 16S rRNA gene-specific markers into separate peaks. A high-resolution CE-SSCP system that uses a poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) triblock copolymer matrix was recently developed and shown to effectively separate highly similar PCR products. In this report, a protocol for the detection of 12 pathogenic bacteria is provided. Pathogen markers were amplified by PCR using universal primers and separated by CE-SSCP; each marker peak was well separated at baseline and showed a characteristic mobility, allowing the easy identification of the pathogens.

  18. Highly biocompatible chitosan with super paramagnetic calcium ferrite (CaFe2O4) nanoparticle for the release of ampicillin.

    Science.gov (United States)

    Bilas, Ram; Sriram, K; Maheswari, P Uma; Sheriffa Begum, K M Meera

    2017-04-01

    The CaFe2O4 nanoparticles (CFNP) were synthesized using the solution combustion method. The CFNP-chitosan-ampicillin was prepared by the ionic gelation method using tripolyphosphate (TPP). The CFNP, chitosan-CFNP, chitosan-CFNP-ampicillin materials were characterized by XRD, FT-IR and TGA analysis in order to evaluate the particle nature and size, the presence of functional groups and their thermal stability. The FESEM and EDAX analysis were performed to understand the surface morphology of the materials and the presence of CFNP in the material, respectively. The vibrating sample magnetometer (VSM) analysis was performed to analyze the magnetic property of the chitosan-CFNP material. The squareness value of 0.1733 obtained by VSM measurements indicates the super paramagnetic nature of chitosan-CFNP. Taguchi orthogonal array method was applied to identify the significant impacting parameters for maximizing the drug encapsulation of chitosan-CFNP. The drug release studies showed that the drug was released rapidly in acidic medium as compared to the basic or neutral medium. The drug release kinetic data were fitted with different linear kinetic model equations and the best fit was obtained with Korsmeyer-Peppas model. The model drug ampicillin release from chitosan-CFNP was tested against staphylococcus epidermis bacteria through disc diffusion method for checking biocompatibility and antibacterial activity.

  19. A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies

    Directory of Open Access Journals (Sweden)

    Charbonnel Nathalie

    2010-05-01

    Full Text Available Abstract Background High-throughput sequencing technologies offer new perspectives for biomedical, agronomical and evolutionary research. Promising progresses now concern the application of these technologies to large-scale studies of genetic variation. Such studies require the genotyping of high numbers of samples. This is theoretically possible using 454 pyrosequencing, which generates billions of base pairs of sequence data. However several challenges arise: first in the attribution of each read produced to its original sample, and second, in bioinformatic analyses to distinguish true from artifactual sequence variation. This pilot study proposes a new application for the 454 GS FLX platform, allowing the individual genotyping of thousands of samples in one run. A probabilistic model has been developed to demonstrate the reliability of this method. Results DNA amplicons from 1,710 rodent samples were individually barcoded using a combination of tags located in forward and reverse primers. Amplicons consisted in 222 bp fragments corresponding to DRB exon 2, a highly polymorphic gene in mammals. A total of 221,789 reads were obtained, of which 153,349 were finally assigned to original samples. Rules based on a probabilistic model and a four-step procedure, were developed to validate sequences and provide a confidence level for each genotype. The method gave promising results, with the genotyping of DRB exon 2 sequences for 1,407 samples from 24 different rodent species and the sequencing of 392 variants in one half of a 454 run. Using replicates, we estimated that the reproducibility of genotyping reached 95%. Conclusions This new approach is a promising alternative to classical methods involving electrophoresis-based techniques for variant separation and cloning-sequencing for sequence determination. The 454 system is less costly and time consuming and may enhance the reliability of genotypes obtained when high numbers of samples are studied

  20. 超高层建筑钢结构测量技术%Steel structure measurement technology of super-high-rise building

    Institute of Scientific and Technical Information of China (English)

    李健良

    2016-01-01

    以广州越秀金融大厦为例,从测量控制点引测、施工测量放样、钢结构焊接后检测等方面,对该项目的钢结构安装测量施工技术进行了论述,为今后超高层建筑的钢结构测量提供了参考。%Taking Yuexiu finance mansion in Guangzhou as an example,starting from aspects of measurement control point leading measurement, construction sampling and steel structure detection after wielding,the thesis discusses the steel structure installation measurement construction technologies,which has provided some guidance for steel structure measurement of super-high-rise building.

  1. Temperature field in the hot-top during casting a new super-high strength Al-Zn-Mg-Cu alloy by low frequency electromagnetic process

    Directory of Open Access Journals (Sweden)

    Yubo ZUO

    2005-08-01

    Full Text Available The billets of a new super-high strength Al-Zn-Mg-Cu alloy in 200 mm diameter were produced by the processed of low frequency electromagnetic casting (LFEC and conventional direct chill(DCcasting, respectively. The effects of low frequency electromagnetic field on temperature field of the melt in the hot-top were investigated by temperature thermocouples into the casting during the processes. The results show that during LFEC process the temperature field in the melt applying the hot-top is very uniform, which is helpful to reduce the difference of thermal gradients between the surface and the center, and then to reduce the thermal stress and to eliminate casting crack.

  2. Temperature field in the hot-top during casting a new super-high strength Al-Zn-Mg-Cu alloy by low frequency electromagnetic process

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The billets of a new super-high strength Al-Zn-Mg-Cu alloy in 200mm diameter were produced by the processes of low frequency electromagnetic casting (LFEC) and conventional direct chill (DC) casting, respectively. The effects of low frequency electromagnetic field on temperature field of the melt in the hot-top were investigated by temperature measurement method. Temperature curves were measured from the surface to the center of the billets by locating type K thermocouples into the casting during the processes. The results show that during LFEC process the temperature field in the melt applying the hot-top is very uniform, which is helpful to reduce the difference of thermal gradients between the surface and the center, and then to reduce the thermal stress and to eliminate casting crack.

  3. Discussion on sustainable management of super high-rise building%谈超高层建筑的可持续化管理

    Institute of Scientific and Technical Information of China (English)

    陆美玲

    2015-01-01

    概括了中国超高层建筑的设计新理念,结合上海中心大厦的建造实例,分别对智能化建筑管理、节能管理、安全与服务管理等可持续化管理经验进行阐述,以期为未来使用者提供安全、舒适、便捷的工作与生活环境。%This article summarizes the new concept of Chinese super high-rise building design. Combined with the examples of Shanghai Tower, this paper elaborates the sustainable management experience in intelligent building management,energy management,security and service man-agement. It wants to provide safety,comfortable and convenient working and living environment for the future users.

  4. Super Tomboy Style

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Sparked by Super Girl, the androgynous look is in among Chinese youth On September 8, this year's top six contestants on the Super Girl television show, a singing contest for young women, stepped into the spotlight. Nearly none of them had long black hair or wore evening gowns, traditionally associated with beauty in China. Rather, they

  5. Identification of high-risk Listeria monocytogenes serotypes in lineage I (serotype 1/2a, 1/2c, 3a and 3c) using multiplex PCR

    Science.gov (United States)

    Aims: Using molecular subtyping techniques, Listeria monocytogenes is divided into three major phylogenetic lineages, and a multiplex PCR method can differentiate five L. monocytogenes subgroups: 1/2a-3a, 1/2c-3c, 1/2b-3b-7, 4b-4d-4e, and 4a-4c. In the current study, we conducted genome comparison...

  6. A highly sensitive, multiplex broad-spectrum PCR-DNA-enzyme immunoassay and reverse hybridization assay for rapid detection and identification of Chlamydia trachomatis serovars.

    NARCIS (Netherlands)

    Quint, K.D.; Doorn, L.J. van; Kleter, B.; Koning, M.N. de; Munckhof, H.A. van den; Morre, S.A.; Harmsel, B. ter; Weiderpass, E.; Harbers, G.; Melchers, W.J.G.; Quint, W.G.V.

    2007-01-01

    Chlamydia trachomatis (Ct) comprises distinct serogroups and serovars. The present study evaluates a novel Ct amplification, detection, and genotyping method (Ct-DT assay). The Ct-DT amplification step is a multiplex broad-spectrum PCR for the cryptic plasmid and the VD2-region of ompl. The Ct-DT de

  7. High throughput multiplex-PCR for direct detection and diagnosis of dermatophyte species, Candida albicans and Candida parapsilosis in clinical specimen.

    Science.gov (United States)

    Vahidnia, Ali; Bekers, Wouter; Bliekendaal, Harry; Spaargaren, Joke

    2015-06-01

    We have developed and validated a multiplex-PCR method for detection of dermatophyte spp., Candida albicans and parapsilosis for routine diagnostics. Our m-PCR showed excellent concordance with culture results in 475 clinical samples. Through the rapid diagnosis by our m-PCR, clinicians are able to initiate adequate antimycotic therapy much earlier.

  8. Multiplexing oscillatory biochemical signals.

    Science.gov (United States)

    de Ronde, Wiet; ten Wolde, Pieter Rein

    2014-04-01

    In recent years it has been increasingly recognized that biochemical signals are not necessarily constant in time and that the temporal dynamics of a signal can be the information carrier. Moreover, it is now well established that the protein signaling network of living cells has a bow-tie structure and that components are often shared between different signaling pathways. Here we show by mathematical modeling that living cells can multiplex a constant and an oscillatory signal: they can transmit these two signals simultaneously through a common signaling pathway, and yet respond to them specifically and reliably. We find that information transmission is reduced not only by noise arising from the intrinsic stochasticity of biochemical reactions, but also by crosstalk between the different channels. Yet, under biologically relevant conditions more than 2 bits of information can be transmitted per channel, even when the two signals are transmitted simultaneously. These observations suggest that oscillatory signals are ideal for multiplexing signals.

  9. Advanced and flexible multi-carrier receiver architecture for high-count multi-core fiber based space division multiplexed applications

    Science.gov (United States)

    Asif, Rameez

    2016-06-01

    Space division multiplexing (SDM), incorporating multi-core fibers (MCFs), has been demonstrated for effectively maximizing the data capacity in an impending capacity crunch. To achieve high spectral-density through multi-carrier encoding while simultaneously maintaining transmission reach, benefits from inter-core crosstalk (XT) and non-linear compensation must be utilized. In this report, we propose a proof-of-concept unified receiver architecture that jointly compensates optical Kerr effects, intra- and inter-core XT in MCFs. The architecture is analysed in multi-channel 512 Gbit/s dual-carrier DP-16QAM system over 800 km 19-core MCF to validate the digital compensation of inter-core XT. Through this architecture: (a) we efficiently compensates the inter-core XT improving Q-factor by 4.82 dB and (b) achieve a momentous gain in transmission reach, increasing the maximum achievable distance from 480 km to 1208 km, via analytical analysis. Simulation results confirm that inter-core XT distortions are more relentless for cores fabricated around the central axis of cladding. Predominantly, XT induced Q-penalty can be suppressed to be less than 1 dB up-to ‑11.56 dB of inter-core XT over 800 km MCF, offering flexibility to fabricate dense core structures with same cladding diameter. Moreover, this report outlines the relationship between core pitch and forward-error correction (FEC).

  10. Development of a Highly Automated and Multiplexed Targeted Proteome Pipeline and Assay for 112 Rat Brain Synaptic Proteins

    Science.gov (United States)

    Colangelo, Christopher M.; Ivosev, Gordana; Chung, Lisa; Abbott, Thomas; Shifman, Mark; Sakaue, Fumika; Cox, David; Kitchen, Rob R.; Burton, Lyle; Tate, Stephen A; Gulcicek, Erol; Bonner, Ron; Rinehart, Jesse; Nairn, Angus C.; Williams, Kenneth R.

    2015-01-01

    We present a comprehensive workflow for large scale (>1000 transitions/run) label-free LC-MRM proteome assays. Innovations include automated MRM transition selection, intelligent retention time scheduling (xMRM) that improves Signal/Noise by >2-fold, and automatic peak modeling. Improvements to data analysis include a novel Q/C metric, Normalized Group Area Ratio (NGAR), MLR normalization, weighted regression analysis, and data dissemination through the Yale Protein Expression Database. As a proof of principle we developed a robust 90 minute LC-MRM assay for Mouse/Rat Post-Synaptic Density (PSD) fractions which resulted in the routine quantification of 337 peptides from 112 proteins based on 15 observations per protein. Parallel analyses with stable isotope dilution peptide standards (SIS), demonstrate very high correlation in retention time (1.0) and protein fold change (0.94) between the label-free and SIS analyses. Overall, our first method achieved a technical CV of 11.4% with >97.5% of the 1697 transitions being quantified without user intervention, resulting in a highly efficient, robust, and single injection LC-MRM assay. PMID:25476245

  11. Analysis and Research of Multiplexing System

    Institute of Scientific and Technical Information of China (English)

    郭惠玲; 王仝杰; 刘越男

    2001-01-01

    The development of optical transmission was summarized. The multiplexing system was show in detail. The concepts, characteristic, key technology, expand trend and application prospect of frequency-division multiplexing, time-division multiplexing, code-division multiplexing and wave-division multiplexing were illustrated.

  12. Extracting Information from Multiplex Networks

    CERN Document Server

    Iacovacci, Jacopo

    2016-01-01

    Multiplex networks are generalized network structures that are able to describe networks in which the same set of nodes are connected by links that have different connotations. Multiplex networks are ubiquitous since they describe social, financial, engineering and biological networks as well. Extending our ability to analyze complex networks to multiplex network structures increases greatly the level of information that is possible to extract from Big Data. For these reasons characterizing the centrality of nodes in multiplex networks and finding new ways to solve challenging inference problems defined on multiplex networks are fundamental questions of network science. In this paper we discuss the relevance of the Multiplex PageRank algorithm for measuring the centrality of nodes in multilayer networks and we characterize the utility of the recently introduced indicator function $\\widetilde{\\Theta}^{S}$ for describing their mesoscale organization and community structure. As working examples for studying thes...

  13. Clustering Coefficients in Multiplex Networks

    CERN Document Server

    Cozzo, Emanuele; De Domenico, Manlio; Solé, Albert; Arenas, Alex; Gómez, Sergio; Porter, Mason A; Moreno, Yamir

    2013-01-01

    Recent advances in the study of complex networked systems has highlighted that our interconnected world is made of networks that are coupled together through different layers that each stand for one type of interaction or system. Despite this situation, it is traditional to aggregate multiplex data into a single weighted network in order take advantage of existing tools. This is admittedly convenient, but it is also extremely problematic. In this paper, we generalize the concept of clustering coefficients for multiplex networks. We show how the layered structure of multiplex networks introduces a new degree of freedom that has a fundamental effect on transitivity. We compute our new multiplex clustering coefficients for several real multiplex networks and illustrate why generalizing monoplex concepts to multiplex networks must be done with great care.

  14. Multiplex Real-Time PCR Assay with High-Resolution Melting Analysis for Characterization of Antimicrobial Resistance in Neisseria gonorrhoeae.

    Science.gov (United States)

    Donà, Valentina; Kasraian, Sara; Lupo, Agnese; Guilarte, Yuvia N; Hauser, Christoph; Furrer, Hansjakob; Unemo, Magnus; Low, Nicola; Endimiani, Andrea

    2016-08-01

    Resistance to antibiotics used against Neisseria gonorrhoeae infections is a major public health concern. Antimicrobial resistance (AMR) testing relies on time-consuming culture-based methods. Development of rapid molecular tests for detection of AMR determinants could provide valuable tools for surveillance and epidemiological studies and for informing individual case management. We developed a fast (<1.5-h) SYBR green-based real-time PCR method with high-resolution melting (HRM) analysis. One triplex and three duplex reactions included two sequences for N. gonorrhoeae identification and seven determinants of resistance to extended-spectrum cephalosporins (ESCs), azithromycin, ciprofloxacin, and spectinomycin. The method was validated by testing 39 previously fully characterized N. gonorrhoeae strains, 19 commensal Neisseria species strains, and an additional panel of 193 gonococcal isolates. Results were compared with results of culture-based AMR determination. The assay correctly identified N. gonorrhoeae and the presence or absence of the seven AMR determinants. There was some cross-reactivity with nongonococcal Neisseria species, and the detection limit was 10(3) to 10(4) genomic DNA (gDNA) copies/reaction. Overall, the platform accurately detected resistance to ciprofloxacin (sensitivity and specificity, 100%), ceftriaxone (sensitivity, 100%; specificity, 90%), cefixime (sensitivity, 92%; specificity, 94%), azithromycin (sensitivity and specificity, 100%), and spectinomycin (sensitivity and specificity, 100%). In conclusion, our methodology accurately detects mutations that generate resistance to antibiotics used to treat gonorrhea. Low assay sensitivity prevents direct diagnostic testing of clinical specimens, but this method can be used to screen collections of gonococcal isolates for AMR more quickly than current culture-based AMR testing.

  15. A versatile, bar-coded nuclear marker/reporter for live cell fluorescent and multiplexed high content imaging.

    Directory of Open Access Journals (Sweden)

    Irina Krylova

    Full Text Available The screening of large numbers of compounds or siRNAs is a mainstay of both academic and pharmaceutical research. Most screens test those interventions against a single biochemical or cellular output whereas recording multiple complementary outputs may be more biologically relevant. High throughput, multi-channel fluorescence microscopy permits multiple outputs to be quantified in specific cellular subcompartments. However, the number of distinct fluorescent outputs available remains limited. Here, we describe a cellular bar-code technology in which multiple cell-based assays are combined in one well after which each assay is distinguished by fluorescence microscopy. The technology uses the unique fluorescent properties of assay-specific markers comprised of distinct combinations of different 'red' fluorescent proteins sandwiched around a nuclear localization signal. The bar-code markers are excited by a common wavelength of light but distinguished ratiometrically by their differing relative fluorescence in two emission channels. Targeting the bar-code to cell nuclei enables individual cells expressing distinguishable markers to be readily separated by standard image analysis programs. We validated the method by showing that the unique responses of different cell-based assays to specific drugs are retained when three assays are co-plated and separated by the bar-code. Based upon those studies, we discuss a roadmap in which even more assays may be combined in a well. The ability to analyze multiple assays simultaneously will enable screens that better identify, characterize and distinguish hits according to multiple biologically or clinically relevant criteria. These capabilities also enable the re-creation of complex mixtures of cell types that is emerging as a central area of interest in many fields.

  16. 3D multiplexed immunoplasmonics microscopy

    Science.gov (United States)

    Bergeron, Éric; Patskovsky, Sergiy; Rioux, David; Meunier, Michel

    2016-07-01

    Selective labelling, identification and spatial distribution of cell surface biomarkers can provide important clinical information, such as distinction between healthy and diseased cells, evolution of a disease and selection of the optimal patient-specific treatment. Immunofluorescence is the gold standard for efficient detection of biomarkers expressed by cells. However, antibodies (Abs) conjugated to fluorescent dyes remain limited by their photobleaching, high sensitivity to the environment, low light intensity, and wide absorption and emission spectra. Immunoplasmonics is a novel microscopy method based on the visualization of Abs-functionalized plasmonic nanoparticles (fNPs) targeting cell surface biomarkers. Tunable fNPs should provide higher multiplexing capacity than immunofluorescence since NPs are photostable over time, strongly scatter light at their plasmon peak wavelengths and can be easily functionalized. In this article, we experimentally demonstrate accurate multiplexed detection based on the immunoplasmonics approach. First, we achieve the selective labelling of three targeted cell surface biomarkers (cluster of differentiation 44 (CD44), epidermal growth factor receptor (EGFR) and voltage-gated K+ channel subunit KV1.1) on human cancer CD44+ EGFR+ KV1.1+ MDA-MB-231 cells and reference CD44- EGFR- KV1.1+ 661W cells. The labelling efficiency with three stable specific immunoplasmonics labels (functionalized silver nanospheres (CD44-AgNSs), gold (Au) NSs (EGFR-AuNSs) and Au nanorods (KV1.1-AuNRs)) detected by reflected light microscopy (RLM) is similar to the one with immunofluorescence. Second, we introduce an improved method for 3D localization and spectral identification of fNPs based on fast z-scanning by RLM with three spectral filters corresponding to the plasmon peak wavelengths of the immunoplasmonics labels in the cellular environment (500 nm for 80 nm AgNSs, 580 nm for 100 nm AuNSs and 700 nm for 40 nm × 92 nm AuNRs). Third, the developed

  17. Control of Angular Intervals for Angle-Multiplexed Holographic Memory

    Science.gov (United States)

    Kinoshita, Nobuhiro; Muroi, Tetsuhiko; Ishii, Norihiko; Kamijo, Koji; Shimidzu, Naoki

    2009-03-01

    In angle-multiplexed holographic memory, the full width at half maximum of the Bragg selectivity curves is dependent on the angle formed between the medium and incident laser beams. This indicates the possibility of high density and high multiplexing number by varying the angular intervals between adjacent holograms. We propose an angular interval scheduling for closely stacking holograms into medium even when the angle range is limited. We obtained bit error rates of the order of 10-4 under the following conditions: medium thickness of 1 mm, laser beam wavelength of 532 nm, and angular multiplexing number of 300.

  18. Supermassive Black Holes with High Accretion Rates in Active Galactic Nuclei. IV. Hβ Time Lags and Implications for Super-Eddington Accretion

    Science.gov (United States)

    Du, Pu; Hu, Chen; Lu, Kai-Xing; Huang, Ying-Ke; Cheng, Cheng; Qiu, Jie; Li, Yan-Rong; Zhang, Yang-Wei; Fan, Xu-Liang; Bai, Jin-Ming; Bian, Wei-Hao; Yuan, Ye-Fei; Kaspi, Shai; Ho, Luis C.; Netzer, Hagai; Wang, Jian-Min; SEAMBH Collaboration

    2015-06-01

    We have completed two years of photometric and spectroscopic monitoring of a large number of active galactic nuclei (AGNs) with very high accretion rates. In this paper, we report on the result of the second phase of the campaign, during 2013-2014, and the measurements of five new Hβ time lags out of eight monitored AGNs. All five objects were identified as super-Eddington accreting massive black holes (SEAMBHs). The highest measured accretion rates for the objects in this campaign are \\mathscr{\\dot{M}} {\\mkern 1mu} ≳ 200, where \\mathscr{\\dot{M}} {\\mkern 1mu} ={{\\dot{M}}\\bullet }/{{L}Edd}{{c}-2}, {{\\dot{M}}\\bullet } is the mass accretion rates, {{L}Edd} is the Eddington luminosity and c is the speed of light. We find that the Hβ time lags in SEAMBHs are significantly shorter than those measured in sub-Eddington AGNs, and the deviations increase with increasing accretion rates. Thus, the relationship between broad-line region size ({{R}_{Hβ }}) and optical luminosity at 5100 Å, {{R}_{Hβ }}-{{L}5100}, requires accretion rate as an additional parameter. We propose that much of the effect may be due to the strong anisotropy of the emitted slim-disk radiation. Scaling {{R}_{Hβ }} by the gravitational radius of the black hole (BH), we define a new radius-mass parameter (Y) and show that it saturates at a critical accretion rate of \\mathscr{\\dot{M}} {\\mkern 1mu} {{}c}=6˜ 30, indicating a transition from thin to slim accretion disk and a saturated luminosity of the slim disks. The parameter Y is a very useful probe for understanding the various types of accretion onto massive BHs. We briefly comment on implications to the general population of super-Eddington AGNs in the universe and applications to cosmology.

  19. Possible Outcomes of Coplanar High-eccentricity Migration: Hot Jupiters, Close-in Super-Earths, and Counter-orbiting Planets

    Science.gov (United States)

    Xue, Yuxin; Masuda, Kento; Suto, Yasushi

    2017-02-01

    We investigate the formation of close-in planets in near-coplanar eccentric hierarchical triple systems via the secular interaction between an inner planet and an outer perturber (Coplanar High-eccentricity Migration; CHEM). We generalize the previous work on the analytical condition for successful CHEM for point masses interacting only through gravity by taking into account the finite mass effect of the inner planet. We find that efficient CHEM requires that the systems should have m1 ≪ m0 and m1 ≪ m2. In addition to the gravity for point masses, we examine the importance of the short-range forces, and provide an analytical estimate of the migration timescale. We perform a series of numerical simulations in CHEM for systems consisting of a Sun-like central star, giant gas inner planet, and planetary outer perturber, including the short-range forces and stellar and planetary dissipative tides. We find that most of such systems end up with a tidal disruption; a small fraction of the systems produce prograde hot Jupiters (HJs), but no retrograde HJ. In addition, we extend CHEM to super-Earth mass range, and show that the formation of close-in super-Earths in prograde orbits is also possible. Finally, we carry out CHEM simulation for the observed hierarchical triple and counter-orbiting HJ systems. We find that CHEM can explain a part of the former systems, but it is generally very difficult to reproduce counter-orbiting HJ systems.

  20. Nonlinear Super Integrable Couplings of Super Classical-Boussinesq Hierarchy

    Directory of Open Access Journals (Sweden)

    Xiuzhi Xing

    2014-01-01

    Full Text Available Nonlinear integrable couplings of super classical-Boussinesq hierarchy based upon an enlarged matrix Lie super algebra were constructed. Then, its super Hamiltonian structures were established by using super trace identity. As its reduction, nonlinear integrable couplings of the classical integrable hierarchy were obtained.

  1. Functional Multiplex PageRank

    Science.gov (United States)

    Iacovacci, Jacopo; Rahmede, Christoph; Arenas, Alex; Bianconi, Ginestra

    2016-10-01

    Recently it has been recognized that many complex social, technological and biological networks have a multilayer nature and can be described by multiplex networks. Multiplex networks are formed by a set of nodes connected by links having different connotations forming the different layers of the multiplex. Characterizing the centrality of the nodes in a multiplex network is a challenging task since the centrality of the node naturally depends on the importance associated to links of a certain type. Here we propose to assign to each node of a multiplex network a centrality called Functional Multiplex PageRank that is a function of the weights given to every different pattern of connections (multilinks) existent in the multiplex network between any two nodes. Since multilinks distinguish all the possible ways in which the links in different layers can overlap, the Functional Multiplex PageRank can describe important non-linear effects when large relevance or small relevance is assigned to multilinks with overlap. Here we apply the Functional Page Rank to the multiplex airport networks, to the neuronal network of the nematode C. elegans, and to social collaboration and citation networks between scientists. This analysis reveals important differences existing between the most central nodes of these networks, and the correlations between their so-called pattern to success.

  2. Development and implementation of a highly-multiplexed SNP array for genetic mapping in maritime pine and comparative mapping with loblolly pine

    Directory of Open Access Journals (Sweden)

    Garnier-Géré Pauline

    2011-07-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs are the most abundant source of genetic variation among individuals of a species. New genotyping technologies allow examining hundreds to thousands of SNPs in a single reaction for a wide range of applications such as genetic diversity analysis, linkage mapping, fine QTL mapping, association studies, marker-assisted or genome-wide selection. In this paper, we evaluated the potential of highly-multiplexed SNP genotyping for genetic mapping in maritime pine (Pinus pinaster Ait., the main conifer used for commercial plantation in southwestern Europe. Results We designed a custom GoldenGate assay for 1,536 SNPs detected through the resequencing of gene fragments (707 in vitro SNPs/Indels and from Sanger-derived Expressed Sequenced Tags assembled into a unigene set (829 in silico SNPs/Indels. Offspring from three-generation outbred (G2 and inbred (F2 pedigrees were genotyped. The success rate of the assay was 63.6% and 74.8% for in silico and in vitro SNPs, respectively. A genotyping error rate of 0.4% was further estimated from segregating data of SNPs belonging to the same gene. Overall, 394 SNPs were available for mapping. A total of 287 SNPs were integrated with previously mapped markers in the G2 parental maps, while 179 SNPs were localized on the map generated from the analysis of the F2 progeny. Based on 98 markers segregating in both pedigrees, we were able to generate a consensus map comprising 357 SNPs from 292 different loci. Finally, the analysis of sequence homology between mapped markers and their orthologs in a Pinus taeda linkage map, made it possible to align the 12 linkage groups of both species. Conclusions Our results show that the GoldenGate assay can be used successfully for high-throughput SNP genotyping in maritime pine, a conifer species that has a genome seven times the size of the human genome. This SNP-array will be extended thanks to recent sequencing effort using

  3. 超稠油高温调剖封窜技术%High Temperature Profile Control and Channel Blocking Sealing Technology for Super Heavy Oil Researviors

    Institute of Scientific and Technical Information of China (English)

    郝建玉

    2012-01-01

    The cyclic steam stimulation is a recovery method of super heavy oil in Liaohe oilfield. The porosity of Du84 block of Xinglongtai oilfield in super heavy oil reservoir is generally 25% to 30%, air permeability is generally higher than 1306 × 10-3 μm2. Because of its high porosity and high permeability, steam channeling easily occurs to cause that heat energy of the injected steam can not be fully utilized, which can reduce production effect of the steam injection well. Water cut in adjacent well after the steam channeling increases, temperature rises, which can affect the production or cause to shut in well. Steam channeling aggravates casing deformation or damage. The high temperature resistant plugging agent can effectively block high permeability layer, adjust the steam absorption difference between low permeability layer and high permeability layer, change the flow direction of injected steam, which can alleviate the steam channeling, eliminate interference among wells, expand the injected steam swept volume, improve cycle oil production.%蒸汽吞吐开采是辽河油田超稠油主要开采方式.曙光油田杜84块兴隆台超稠油油藏孔隙度一般为25%~30%,空气渗透率一般高于1306×10-3 μm2,具有高孔隙度,高渗透率的特点,极易发生汽窜,导致注入蒸汽热能不能充分利用,直接降低了注汽井生产效果,使油藏动用不均的矛盾加剧.邻井受窜后含水升高液量突升,温度升高,影响其生产效果或关井防喷.汽窜加剧油层套管变形或损坏.研制的耐高温堵剂有效封堵高渗透层,调整地层高低渗透层带间的吸汽差异,改变注入蒸汽的走向,达到缓解汽窜、消除井间干扰、扩大注入蒸汽波及体积、提高周期采油量的目的.

  4. SuperB Progress Report for Physics

    Energy Technology Data Exchange (ETDEWEB)

    O' Leary, B.; /Aachen, Tech. Hochsch.; Matias, J.; Ramon, M.; /Barcelona, IFAE; Pous, E.; /Barcelona U.; De Fazio, F.; Palano, A.; /INFN, Bari; Eigen, G.; /Bergen U.; Asgeirsson, D.; /British Columbia U.; Cheng, C.H.; Chivukula, A.; Echenard, B.; Hitlin, D.G.; Porter, F.; Rakitin, A.; /Caltech; Heinemeyer, S.; /Cantabria Inst. of Phys.; McElrath, B.; /CERN; Andreassen, R.; Meadows, B.; Sokoloff, M.; /Cincinnati U.; Blanke, M.; /Cornell U., Phys. Dept.; Lesiak, T.; /Cracow, INP /DESY /Zurich, ETH /INFN, Ferrara /Frascati /INFN, Genoa /Glasgow U. /Indiana U. /Mainz U., Inst. Phys. /Karlsruhe, Inst. Technol. /KEK, Tsukuba /LBL, Berkeley /UC, Berkeley /Lisbon, IST /Ljubljana U. /Madrid, Autonoma U. /Maryland U. /MIT /INFN, Milan /McGill U. /Munich, Tech. U. /Notre Dame U. /PNL, Richland /INFN, Padua /Paris U., VI-VII /Orsay, LAL /Orsay, LPT /INFN, Pavia /INFN, Perugia /INFN, Pisa /Queen Mary, U. of London /Regensburg U. /Republica U., Montevideo /Frascati /INFN, Rome /INFN, Rome /INFN, Rome /Rutherford /Sassari U. /Siegen U. /SLAC /Southern Methodist U. /Tel Aviv U. /Tohoku U. /INFN, Turin /INFN, Trieste /Uppsala U. /Valencia U., IFIC /Victoria U. /Wayne State U. /Wisconsin U., Madison

    2012-02-14

    SuperB is a high luminosity e{sup +}e{sup -} collider that will be able to indirectly probe new physics at energy scales far beyond the reach of any man made accelerator planned or in existence. Just as detailed understanding of the Standard Model of particle physics was developed from stringent constraints imposed by flavour changing processes between quarks, the detailed structure of any new physics is severely constrained by flavour processes. In order to elucidate this structure it is necessary to perform a number of complementary studies of a set of golden channels. With these measurements in hand, the pattern of deviations from the Standard Model behavior can be used as a test of the structure of new physics. If new physics is found at the LHC, then the many golden measurements from SuperB will help decode the subtle nature of the new physics. However if no new particles are found at the LHC, SuperB will be able to search for new physics at energy scales up to 10-100 TeV. In either scenario, flavour physics measurements that can be made at SuperB play a pivotal role in understanding the nature of physics beyond the Standard Model. Examples for using the interplay between measurements to discriminate New Physics models are discussed in this document. SuperB is a Super Flavour Factory, in addition to studying large samples of B{sub u,d,s}, D and {tau} decays, SuperB has a broad physics programme that includes spectroscopy both in terms of the Standard Model and exotica, and precision measurements of sin{sup 2} {theta}{sub W}. In addition to performing CP violation measurements at the {Upsilon}(4S) and {phi}(3770), SuperB will test CPT in these systems, and lepton universality in a number of different processes. The multitude of rare decay measurements possible at SuperB can be used to constrain scenarios of physics beyond the Standard Model. In terms of other precision tests of the Standard Model, this experiment will be able to perform precision over

  5. Determinants of public cooperation in multiplex networks

    Science.gov (United States)

    Battiston, Federico; Perc, Matjaž; Latora, Vito

    2017-07-01

    Synergies between evolutionary game theory and statistical physics have significantly improved our understanding of public cooperation in structured populations. Multiplex networks, in particular, provide the theoretical framework within network science that allows us to mathematically describe the rich structure of interactions characterizing human societies. While research has shown that multiplex networks may enhance the resilience of cooperation, the interplay between the overlap in the structure of the layers and the control parameters of the corresponding games has not yet been investigated. With this aim, we consider here the public goods game on a multiplex network, and we unveil the role of the number of layers and the overlap of links, as well as the impact of different synergy factors in different layers, on the onset of cooperation. We show that enhanced public cooperation emerges only when a significant edge overlap is combined with at least one layer being able to sustain some cooperation by means of a sufficiently high synergy factor. In the absence of either of these conditions, the evolution of cooperation in multiplex networks is determined by the bounds of traditional network reciprocity with no enhanced resilience. These results caution against overly optimistic predictions that the presence of multiple social domains may in itself promote cooperation, and they help us better understand the complexity behind prosocial behavior in layered social systems.

  6. Preliminary Assessment of Microwave Readout Multiplexing Factor

    Energy Technology Data Exchange (ETDEWEB)

    Croce, Mark Philip [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Koehler, Katrina Elizabeth [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Rabin, Michael W. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Bennett, D. A. [National Inst. of Standards and Technology (NIST), Boulder, CO (United States); Mates, J. A. B. [National Inst. of Standards and Technology (NIST), Boulder, CO (United States); Gard, J. D. [National Inst. of Standards and Technology (NIST), Boulder, CO (United States); Becker, D. [National Inst. of Standards and Technology (NIST), Boulder, CO (United States); Schmidt, D. R. [National Inst. of Standards and Technology (NIST), Boulder, CO (United States); Ullom, J. N. [National Inst. of Standards and Technology (NIST), Boulder, CO (United States)

    2017-01-23

    Ultra-high resolution microcalorimeter gamma spectroscopy is a new non-destructive assay technology for measurement of plutonium isotopic composition, with the potential to reduce total measurement uncertainty to a level competitive with destructive analysis methods [1-4]. Achieving this level of performance in practical applications requires not only the energy resolution now routinely achieved with transition-edge sensor microcalorimeter arrays (an order of magnitude better than for germanium detectors) but also high throughput. Microcalorimeter gamma spectrometers have not yet achieved detection efficiency and count rate capability that is comparable to germanium detectors, largely because of limits from existing readout technology. Microcalorimeter detectors must be operated at low temperature to achieve their exceptional energy resolution. Although the typical 100 mK operating temperatures can be achieved with reliable, cryogen-free systems, the cryogenic complexity and heat load from individual readout channels for large sensor arrays is prohibitive. Multiplexing is required for practical systems. The most mature multiplexing technology at present is time-division multiplexing (TDM) [3, 5-6]. In TDM, the sensor outputs are switched by applying bias current to one SQUID amplifier at a time. Transition-edge sensor (TES) microcalorimeter arrays as large as 256 pixels have been developed for X-ray and gamma-ray spectroscopy using TDM technology. Due to bandwidth limits and noise scaling, TDM is limited to a maximum multiplexing factor of approximately 32-40 sensors on one readout line [8]. Increasing the size of microcalorimeter arrays above the kilopixel scale, required to match the throughput of germanium detectors, requires the development of a new readout technology with a much higher multiplexing factor.

  7. λ-Selection Strategy in C+L Band 1-Pbit/s (448 WDM/19-Core/128 Gbit/s/channel) Flex-Grid Space Division Multiplexed Transmission

    DEFF Research Database (Denmark)

    Asif, Rameez; Ye, Feihong; Morioka, Toshio

    2015-01-01

    In this paper, an inter-core crosstalk based wavelength selection scheme has been proposed for flex-grid superchannels in space division multiplexed transmission. The two λ-selection strategies are categorized as: (a) aligned wavelength super-channels (Aλ-SCs), where all super-channels are placed...

  8. Diversity-Multiplexing Tradeoff in the Low-SNR Regime

    CERN Document Server

    Loyka, Sergey

    2011-01-01

    An extension of the popular diversity-multiplexing tradeoff framework to the low-SNR (or wideband) regime is proposed. The concept of diversity gain is shown to be redundant in this regime since the outage probability is SNR-independent and depends on the multiplexing gain and the channel power gain statistics only. The outage probability under the DMT framework is obtained in an explicit, closed form for a broad class of channels. The low and high-SNR regime boundaries are explicitly determined for the scalar Rayleigh-fading channel, indicating a significant limitation of the SNR-asymptotic DMT when the multiplexing gain is small.

  9. Multiplex detection of RNA expression in Drosophila embryos.

    Science.gov (United States)

    Kosman, Dave; Mizutani, Claudia M; Lemons, Derek; Cox, W Gregory; McGinnis, William; Bier, Ethan

    2004-08-06

    We present a fluorescence-based, multiplex in situ hybridization method that permits the simultaneous detection of five differently labeled antisense RNA probes and up to seven differ-ent transcripts in a single Drosophila embryo. We also show that it should be possible to increase the number of detected transcripts substantially with nascent transcript multiplex fluorescent in situ hybridization. These multiplex methods fill a current technological gap between high-resolution in situ hybridization with one or two fluorescently labeled probes and low-resolution but genome-wide microarray RNA profiling and should be of great utility in establishing gene networks.

  10. Experimental demonstration of subcarrier multiplexed quantum key distribution system.

    Science.gov (United States)

    Mora, José; Ruiz-Alba, Antonio; Amaya, Waldimar; Martínez, Alfonso; García-Muñoz, Víctor; Calvo, David; Capmany, José

    2012-06-01

    We provide, to our knowledge, the first experimental demonstration of the feasibility of sending several parallel keys by exploiting the technique of subcarrier multiplexing (SCM) widely employed in microwave photonics. This approach brings several advantages such as high spectral efficiency compatible with the actual secure key rates, the sharing of the optical fainted pulse by all the quantum multiplexed channels reducing the system complexity, and the possibility of upgrading with wavelength division multiplexing in a two-tier scheme, to increase the number of parallel keys. Two independent quantum SCM channels featuring a sifted key rate of 10 Kb/s/channel over a link with quantum bit error rate <2% is reported.

  11. NETL Super Computer

    Data.gov (United States)

    Federal Laboratory Consortium — The NETL Super Computer was designed for performing engineering calculations that apply to fossil energy research. It is one of the world’s larger supercomputers,...

  12. 超高压气井站场控制系统研究%Research on Control System of Super-high Pressure Gas Well

    Institute of Scientific and Technical Information of China (English)

    周晓; 四名; 邹凯

    2016-01-01

    This paper introduces the design of a RTU control system for super-high pressure gas wel based on the control wave redundant structure,integrated application of auto-control and touch screen terminal technology,with the function of output regulation,parameter monitoring and intel igent alarming,simulating manual operation steps,shut down the wel through pro-grammed language.The practical application shows that the system has the advantages of high stability and high intel igence.%介绍了一种基于CONTROL WAVE冗余架构的超高压气井RTU控制系统设计,集成运用自动化控制技术和触摸屏终端技术于一体,实现超高压气井的产量调节、参数监控和智能报警功能,模拟人工现场操作步骤,通过程序化的语言实现远程关井作业。实践应用表明该系统具有稳定性高、智能化程度高的优点。

  13. Super-Kamiokande

    Science.gov (United States)

    Magro, Lluís Martí

    2016-06-01

    The Super-Kamiokande experiment performs a large variety of studies, many of them in the neutrino sector. The archetypes are atmospheric neutrino (recently awarded with the Nobel prize for Mr. T. Kajita) and the solar neutrinos analyses. In these proceedings we report our latest results and present updates to indirect dark matter searches, our solar neutrino analysis and discuss the future upgrade of Super-Kamiokande by loading gadolinium into our ultra-pure water.

  14. The Super Girl Effect

    Institute of Scientific and Technical Information of China (English)

    WANG PEI

    2006-01-01

    @@ In recent years, Changsha,the capital city of Hunan Province, has become famous across China for its innovative TV channel, in particular the cultural phenomenon of the Super Girl talent show. And as far as culture goes, Hunan TV is merely a reflection of a renaissance happening in the city. Animation, music halls, drama festivals and a famous book market are just some of the city's cultural sectors that are benefiting from the fame and notoriety of the Super Girl show.

  15. The formation of super-rings

    CERN Document Server

    Tenorio-Tagle, G

    1980-01-01

    The author has calculated the collision of a small neutral cloud (surface density approximately 10/sup 19/ cm/sup -2/) with a constant density galactic disk. Through the collision, a large amount of energy is deposited in a small volume of the galaxy, resulting in a supersonic expansion of very hot (10/sup 6/-10/sup 7/K) gas into the Galaxy and out of the galactic disk. The expansion generates a large cavity (a super-ring) with physical characteristics (diameter, velocity of expansion, etc.) in agreement with the observations, and a large volume of hot low-density gas with properties similar to those of the observed coronal gas. (31 refs).

  16. Nonlinear Super Integrable Couplings of Super Dirac Hierarchy and Its Super Hamiltonian Structures

    Institute of Scientific and Technical Information of China (English)

    尤福财

    2012-01-01

    We construct nonlinear super integrable couplings of the super integrable Dirac hierarchy based on an enlarged matrix Lie superalgebra. Then its super Hamiltonian structure is furnished by super trace identity. As its reduction, we gain the nonlinear integrable couplings of the classical integrable Dirac hierarchy.

  17. Multidiameter optical ring and Hermite-Gaussian vortices for wavelength division multiplexing-mode division multiplexing

    Science.gov (United States)

    Amphawan, Angela; Fazea, Yousef

    2016-10-01

    Optical vortices are high-capacity data carriers for mode division multiplexing (MDM) in multimode fiber (MMF). This paper reports on the MDM of a combination of helical-phased optical vortices comprising donut modes and Hermite-Gaussian (HG) modes for different radial offsets from the MMF axis. A data rate of 44 Gbps is achieved for wavelength division multiplexing-MDM of two pairs of helical-phased donut mode and HG mode at wavelengths 1550.12 and 1551.72 nm for a MMF length of 1500 m.

  18. Integrated SiC Super Junction Transistor-Diode Devices for High-Power Motor Control ModulesOoperating at 500 C Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Monolithic Integrated SiC Super Junction Transistor-JBS diode (MIDSJT) devices are used to construct 500

  19. Quartz splintTM高强度石英纤维和Super-Bond C & B粘结剂固定下前牙松动牙疗效比较%Comparison of the clinical effect of Quartz splintTM high strength quartz fiber and Super-Bond C & B adhesive for anterior teeth loosening

    Institute of Scientific and Technical Information of China (English)

    赵静

    2016-01-01

    目的:比较Quartz splintTM高强度石英纤维和Super-Bond C&B粘结剂固定慢性牙周炎下前牙松动牙的临床效果。方法:选取下前牙松动需要做牙周夹板松牙固定患者40例,随机分为两组。A组:在洁治、刮治和根面平整后,使用Quartz splintTM高强度石英纤维做牙周固定;B组:在在洁治、刮治和根面平整后,使用Super-Bond C&B粘结剂做牙周固定。两组固定后即刻让患者对于固定物做出异物感评价。固定6个月后比较治疗前后的牙周探诊深度和牙周附着丧失情况。结果:两组治疗前后的牙周探诊深度和牙周附着丧失均比治疗前有明显减少,有统计学意义。组间比较,无统计学意义。固定后即刻异物感评价,B组优于A组,有统计学意义。结论:对于下前牙松动的患者,洁治、刮治和根面平整后使用Quartz splintTM高强度石英纤维和Super-Bond C&B粘结剂牙周夹板固定,均可以使牙周状况明显改善。Super-Bond C&B粘结剂牙周夹板异物感小于Quartz splintTM高强度石英纤维。%Objective To compare the clinical effect of Quartz splintTM high strength silica fiber and Super-Bond C&B adhesive in the treatment of anterior teeth loosening of the periodontal disease. Methods 40 patients with anterior teeth loosening need to be fixed in patients with loose teeth, and were randomly divided into two groups. In group A, scaling and root planing, the use of Quartz splintTM high strength quartz fiber periodontal fixed. In group B, scaling, root planing, the use of Super-Bond C&B adhesive periodontal fixation. A group and B group make foreign body sensation evaluation after fixation immediately. After 6 months of treatment, the depth of the periodontal probe and the loss of periodontal attachment were compared with that before and after the treatment. Results The depth of the periodontal probe and the loss of periodontal attachment in the A group and the B

  20. Detection of α-thalassemia-1 Southeast Asian and Thai Type Deletions and β-thalassemia 3.5-kb Deletion by Single-tube Multiplex Real-time PCR with SYBR Green1 and High-resolution Melting Analysis

    OpenAIRE

    Pornprasert, Sakorn; Wiengkum, Thanatcha; Srithep, Sarinee; Chainoi, Isarapong; Singboottra, Panthong; Wongwiwatthananukit, Sanchai

    2011-01-01

    Background Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia. Methods Single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting (HRM) analysis were used for the identification of α-thalassemia-1 Southeast Asian (SEA) and Thai type deletions and β-thalassemia 3.5-kb gene deletion. The results were compared with those obtained using conventional gap-P...

  1. Linear analysis on the onset of thermal convection of highly compressible fluids: implications for the mantle convection of super-Earths

    Science.gov (United States)

    Kameyama, Masanori; Miyagoshi, Takehiro; Ogawa, Masaki

    2015-02-01

    A series of linear analysis was performed on the onset of thermal convection of highly compressible fluids, in order to deepen the fundamental insights into the mantle convection of massive super-Earths in the presence of strong adiabatic compression. We consider the temporal evolution (growth or decay) of an infinitesimal perturbation superimposed to a highly compressible fluid which is in a hydrostatic (motionless) and conductive state in a basally heated horizontal layer. As a model of pressure-dependence in material properties, we employed an exponential decrease in thermal expansivity α and exponential increase in (reference) density ρ with depth. The linearized equations for conservation of mass, momentum and internal (thermal) energy are numerically solved for the critical Rayleigh number as well as the vertical profiles of eigenfunctions for infinitesimal perturbations. The above calculations are repeatedly carried out by systematically varying (i) the dissipation number (Di), (ii) the temperature at the top surface and (iii) the magnitude of pressure-dependence in α and ρ. Our analysis demonstrated that the onset of thermal convection is strongly affected by the adiabatic compression, in response to the changes in the static stability of thermal stratification in the fluid layer. For sufficiently large Di where a thick sublayer of stable stratification develops in the layer, for example, the critical Rayleigh number explosively increases with Di, together with drastic decreases in the length scales of perturbations both in vertical and horizontal directions. In particular, for very large Di, a thick `stratosphere' occurs in the fluid layer where the vertical motion is significantly suppressed, resulting in a shrink of the incipient convection in a thin sublayer of unstable thermal stratification. In addition, when Di exceeds a threshold value above which a thermal stratification becomes stable in the entire layer, no perturbation is allowed to grow

  2. 超高层建筑群绿色施工综合应用技术%Comprehensive Technology Applied in Green Construction on Super High Rise Buildings

    Institute of Scientific and Technical Information of China (English)

    周睿; 禹航; 赵淑容

    2014-01-01

    The Western International Financial Center project paid high attention to green construction. Resources like water,land,energy,materials and pollution have been effectively controlled,especially the three applied innovative technologies about waste treatment,mobile toilet,concrete pump cleaning were carried out on super high rise buildings,which achieved greater level on green construction.%西部国际金融中心项目高度重视绿色施工,对水资源、土地资源、能源、材料和环境污染等进行有效控制,尤其项目实施的超高层垃圾管道、临时厕所、混凝土洗泵管3项创新改良后的施工技术,使项目绿色施工达到更为良好的效果。

  3. Spectral responsivity calibration of the reference radiation thermometer at KRISS by using a super-continuum laser-based high-accuracy monochromatic source

    Science.gov (United States)

    Yoo, Yong Shim; Kim, Gun Jung; Park, Seongchong; Lee, Dong-Hoon; Kim, Bong-Hak

    2016-12-01

    We report on the calibration of the relative spectral responsivity of the reference radiation thermometer, model LP4, which is used for the experimental realisation of the international temperature scale of 1990 above 960 °C at the Korea Research Institute of Standards and Science. The relative spectral responsivity of LP4 is measured by using a monochromatic source consisting of a super-continuum laser and a double-grating monochromator. By monitoring the wavelength of the output beam directly with a calibrated wavelength-meter, we achieved a high-accuracy measurement of spectral responsivity with a maximum wavelength error of less than 3 pm, a narrow spectral bandwidth of less than 0.4 nm, and a high dynamic range over 8 decades. We evaluated the contributions of various uncertainty components of the spectral responsivity measurement to the uncertainty of the temperature scale based on a practical estimation approach, which numerically calculates the maximal effects of the variations of each component. As a result, we evaluate the uncertainty contribution from the spectral responsivity measurement to the temperature scale to be less than 64 mK (k  =  1) in a range from 660 °C to 2749 °C for the LP4 with a filter at 650 nm.

  4. Feedback in the Antennae Galaxies (NGC 4038/9): I. High-Resolution Infrared Spectroscopy of Winds from Super Star Clusters

    Energy Technology Data Exchange (ETDEWEB)

    Gilbert, A; Graham, J

    2007-06-05

    We present high-resolution (R {approx} 24,600) near-IR spectroscopy of the youngest super star clusters (SSCs) in the prototypical starburst merger, the Antennae Galaxies. These SSCs are young (3-7 Myr old) and massive (10{sup 5}-10{sup 7} M{sub {circle_dot}} for a Kroupa IMF) and their spectra are characterized by broad, extended Brackett {gamma} emission, so we refer to them as emission-line clusters (ELCs) to distinguish them from older SSCs. The Br {gamma} lines of most ELCs have supersonic widths (60-110 km s{sup -1} FWHM) and non-Gaussian wings whose velocities exceed the clusters escape velocities. This high-velocity unbound gas is flowing out in winds that are powered by the clusters massive O and W-R stars over the course of at least several crossing times. The large sizes of some ELCs relative to those of older SSCs may be due to expansion caused by these outflows; many of the ELCs may not survive as bound stellar systems, but rather dissipate rapidly into the field population. The observed tendency of older ELCs to be more compact than young ones is consistent with the preferential survival of the most concentrated clusters at a given age.

  5. Photochemical properties in flag leaves of a super-high-yielding hybrid rice and a traditional hybrid rice (Oryza sativa L.) probed by chlorophyll a fluorescence transient.

    Science.gov (United States)

    Zhang, Meiping; Shan, YongJie; Kochian, Leon; Strasser, Reto J; Chen, GuoXiang

    2015-12-01

    Chlorophyll a fluorescence of flag leaves in a super-high-yielding hybrid rice (Oryza sativa L.) LYPJ, and a traditional hybrid rice SY63 cultivar with lower grain yield, which were grown in the field, were investigated from emergence through senescence of flag leaves. As the flag leaf matured, there was an increasing trend in photosynthetic parameters such as quantum efficiency of primary photochemistry ([Formula: see text] Po) and efficiency of electron transport from PS II to PS I (Ψ Eo). The overall photosynthetic performance index (PIABS) was significantly higher in the high-yielding LYPJ compared to SY63 during the entire reproductive stage of the plant, the same to MDA content. However, [Formula: see text] Po(=F V/F M), an indicator of the primary photochemistry of the flag leaf, did not display significant changes with leaf age and was not significantly different between the two cultivars, suggesting that PIABS is a more sensitive parameter than [Formula: see text] Po (=F V/F M) during leaf age for distinguishing between cultivars differing in yield.

  6. A super-high angular resolution principle for coded-mask X-ray imaging beyond the diffraction limit of a single pinhole

    Institute of Scientific and Technical Information of China (English)

    Chen Zhang; Shuang-Nan Zhang

    2009-01-01

    High angular resolution X-ray imaging is always useful in astrophysics and solar physics. In principle, it can be performed by using coded-mask imaging with a very long mask-detector distance. Previously, the diffraction-interference effect was thought to degrade coded-mask imaging performance dramatically at the low energy end with its very long mask-detector distance. The diffraction-interference effect is described with numerical calculations, and the difffraction-interference cross correlation reconstruction method (DICC) is developed in order to overcome the imaging performance degradation. Based on the DICC, a super-high angular resolution principle (SHARP) for coded-mask X-ray imaging is proposed. The feasibility of coded mask imaging beyond the diffraction limit of a single pinhole is demonstrated with simulations. With the specification that the mask element size is 50 × 50 μm2 and the mask-detector distance is 50 m, the achieved angular resolution is 0.32arcsec above about 10keV and 0.36arcsec at 1.24keV (λ = 1 nm), where diffraction cannot be neglected. The on-axis source location accuracy is better than 0.02 arcsec. Potential applications for solar observations and wide-field X-ray monitors are also briefly discussed.

  7. A Super-high Angular Resolution Principle for Coded-mask X-ray Imaging Beyond the Diffraction Limit of Single Pinhole

    CERN Document Server

    Zhang, Chen

    2008-01-01

    High angular resolution X-ray imaging is always demanded by astrophysics and solar physics, which can be realized by coded-mask imaging with very long mask-detector distance in principle. Previously the diffraction-interference effect has been thought to degrade coded-mask imaging performance dramatically at low energy end with very long mask-detector distance. In this work the diffraction-interference effect is described with numerical calculations, and the diffraction-interference cross correlation reconstruction method (DICC) is developed in order to overcome the imaging performance degradation. Based on the DICC, a super-high angular resolution principle (SHARP) for coded-mask X-ray imaging is proposed. The feasibility of coded mask imaging beyond the diffraction limit of single pinhole is demonstrated with simulations. With the specification that the mask element size of 50* 50 square micrometers and the mask-detector distance of 50 m, the achieved angular resolution is 0.32 arcsec above about 10 keV, an...

  8. Properties of High Redshift Quasars-II: What does the quasar luminosity function tell us about super-massive black-hole evolution?

    CERN Document Server

    Wyithe, S; Wyithe, Stuart

    2006-01-01

    In the local universe, the masses of Super-Massive Black-Holes (SMBH) appear to correlate with the physical properties of their hosts, including the mass of the dark-matter halo. Using these clues as a starting point many studies have produced models that can explain phenomena like the quasar luminosity function. The shortcoming of this approach is that working models are not unique, and as a result it is not always clear what input physics is being constrained. Here we take a different approach. We identify critical parameters that describe the evolution of SMBHs at high redshift, and constrain their parameter space based on observations of high redshift quasars from the Sloan Digital Sky Survey. We find that the luminosity function taken in isolation is somewhat limited in its ability to constrain SMBH evolution due to some strong degeneracies. This explains the presence in the literature of a range of equally successful models based on different physical hypotheses. Including the constraint of the local SM...

  9. Avaliação da eficácia clínica de uma nova modalidade de fototerapia utilizando diodos emissores de luz Efficacy of new microprocessed phototherapy system with five high intensity light emitting diodes (Super LED

    Directory of Open Access Journals (Sweden)

    Bianca M. R. Martins

    2007-06-01

    Full Text Available OBJETIVO: Avaliar a eficácia terapêutica de um sistema de fototerapia microprocessada que utiliza diodos emissores de luz (Super LED de alta intensidade no tratamento da hiperbilirrubinemia em recém-nascidos prematuros. MÉTODOS: Ensaio clínico, randomizado e controlado, utilizando a fototerapia Super LED no grupo experimental e duas fototerapias halógenas no grupo controle. A randomização foi realizada em blocos e estratificada por peso de nascimento. A duração da fototerapia e a queda nos níveis séricos de bilirrubina total nas primeiras 24 horas de tratamento foram os principais desfechos analisados. RESULTADOS: Foram estudados 88 recém-nascidos, 44 no grupo da fototerapia Super LED e 44 no grupo da fototerapia halógena. As características demográficas da população foram semelhantes nos dois grupos. O nível sérico médio inicial de bilirrubina no grupo do Super LED (10,1±2,4 mg% foi semelhante ao do grupo que recebeu fototerapia halógena (10,9±2,0 mg%. A queda percentual na concentração sérica de bilirrubina total nas primeiras 24 horas de tratamento foi significativamente maior (27,9 versus 10,7%, p OBJECTIVES: To evaluate the efficacy of a microprocessed phototherapy (PT system with five high intensity light emitting diodes (Super LED for the treatment of neonatal hyperbilirubinemia of premature infants. METHODS: Randomized clinical trial using Super LED phototherapy in the study group and twin halogen spotlight phototherapy in the control group. A stratified blocked randomization, based on birth weight, was performed. The duration of phototherapy and the rate of decrease of total serum bilirubin (TSB concentration in the first 24 hours of treatment were the main outcome measures. RESULTS: We studied 88 infants, 44 in the Super LED group and 44 in the halogen spotlight PT group. The demographic characteristics of the patients in both groups were similar. Infants in the Super LED group had a similar mean initial

  10. Detection of α-thalassemia-1 Southeast Asian and Thai Type Deletions and β-thalassemia 3.5-kb Deletion by Single-tube Multiplex Real-time PCR with SYBR Green1 and High-resolution Melting Analysis

    Science.gov (United States)

    Wiengkum, Thanatcha; Srithep, Sarinee; Chainoi, Isarapong; Singboottra, Panthong; Wongwiwatthananukit, Sanchai

    2011-01-01

    Background Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia. Methods Single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting (HRM) analysis were used for the identification of α-thalassemia-1 Southeast Asian (SEA) and Thai type deletions and β-thalassemia 3.5-kb gene deletion. The results were compared with those obtained using conventional gap-PCR. DNA samples were derived from 28 normal individuals, 11 individuals with α-thalassemia-1 SEA type deletion, 2 with α-thalassemia-1 Thai type deletion, and 2 with heterozygous β-thalassemia 3.5-kb gene deletion. Results HRM analysis indicated that the amplified fragments from α-thalassemia-1 SEA type deletion, α-thalassemia-1 Thai type deletion, β-thalassemia 3.5-kb gene deletion, and the wild-type β-globin gene had specific peak heights at mean melting temperature (Tm) values of 86.89℃, 85.66℃, 77.24℃, and 74.92℃, respectively. The results obtained using single-tube multiplex real-time PCR with SYBR Green1 and HRM analysis showed 100% consistency with those obtained using conventional gap-PCR. Conclusions Single-tube multiplex real-time PCR with SYBR Green1 and HRM analysis is a potential alternative for routine clinical screening of the common types of α- and β-thalassemia large gene deletions, since it is simple, cost-effective, and highly accurate. PMID:21779184

  11. Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper and escape mutant (clade 2.2.1 variant lineages in Egypt

    Directory of Open Access Journals (Sweden)

    Arafa Abdel-Satar

    2010-10-01

    Full Text Available Abstract Background The endemic status of highly pathogenic avian influenza virus (HPAIV of subtype H5N1 in Egypt continues to devastate the local poultry industry and poses a permanent threat for human health. Several genetically and antigenically distinct H5N1 lineages co-circulate in Egypt: Strains of clade 2.2.1 proper replicate mainly in backyard birds causing the bulk of human infections, while a variant lineage within 2.2.1 (2.2.1v appears to be perpetuated mainly in commercial poultry farms in Egypt. Viruses of the 2.2.1v lineage represent drift variants escaping from conventional vaccine-induced immunity and some of these strains also escaped detection by commercial real time reverse transcriptase PCR (RT-qPCR protocols due to mismatches in the primers/probe binding sites. Results We developed therefore a versatile, sensitive and lineage-specific multiplex RT-qPCR for detection and typing of H5N1 viruses in Egypt. Analytical characterization was carried out using 50 Egyptian HPAIV H5N1 strains isolated since 2006 and 45 other avian influenza viruses (AIV. A detection limit of 400 cRNA copies per ml sample matrix was found. Higher diagnostic sensitivity of the multiplex assay in comparison to other generic H5 or M-gene based RT-qPCR assays were found by examination of 63 swab samples from experimentally infected chickens and 50 AIV-positive swab samples from different host species in the field in Egypt. Conclusions The new multiplex RT-qPCR assay could be useful for rapid high-throughput monitoring for the presence of HPAIV H5N1 in commercial poultry in Egypt. It may also aid in prospective epidemiological studies to further delineate and better control spread of HPAIV H5N1 in Egypt.

  12. Deforming super Riemann surfaces with gravitinos and super Schottky groups

    Energy Technology Data Exchange (ETDEWEB)

    Playle, Sam [Dipartimento di Fisica, Università di Torino and INFN, Sezione di Torino,Via P. Giuria 1, I-10125 Torino (Italy)

    2016-12-12

    The (super) Schottky uniformization of compact (super) Riemann surfaces is briefly reviewed. Deformations of super Riemann surface by gravitinos and Beltrami parameters are recast in terms of super Schottky group cohomology. It is checked that the super Schottky group formula for the period matrix of a non-split surface matches its expression in terms of a gravitino and Beltrami parameter on a split surface. The relationship between (super) Schottky groups and the construction of surfaces by gluing pairs of punctures is discussed in an appendix.

  13. Super-Eccentric Migrating Jupiters

    CERN Document Server

    Socrates, Aristotle; Dong, Subo; Tremaine, Scott

    2011-01-01

    An important class of formation theories for hot Jupiters involves the excitation of extreme orbital eccentricity (e=0.99 or even larger) followed by tidal dissipation at periastron passage that eventually circularizes the planetary orbit at a period less than 10 days. In a steady state, this mechanism requires the existence of a significant population of super-eccentric (e>0.9) migrating Jupiters with long orbital periods and periastron distances of only a few stellar radii. For these super-eccentric planets, the periastron is fixed due to conservation of orbital angular momentum and the energy dissipated per orbit is constant, implying that the rate of change in semi-major axis a is \\dot a \\propto a^0.5 and consequently the number distribution satisfies dN/dlog a\\propto a^0.5. If this formation process produces most hot Jupiters, Kepler should detect several super-eccentric migrating progenitors of hot Jupiters, allowing for a test of high-eccentricity migration scenarios.

  14. SUPER-ECCENTRIC MIGRATING JUPITERS

    Energy Technology Data Exchange (ETDEWEB)

    Socrates, Aristotle; Katz, Boaz; Dong Subo; Tremaine, Scott [Institute for Advanced Study, Princeton, NJ 08540 (United States)

    2012-05-10

    An important class of formation theories for hot Jupiters involves the excitation of extreme orbital eccentricity (e = 0.99 or even larger) followed by tidal dissipation at periastron passage that eventually circularizes the planetary orbit at a period less than 10 days. In a steady state, this mechanism requires the existence of a significant population of super-eccentric (e > 0.9) migrating Jupiters with long orbital periods and periastron distances of only a few stellar radii. For these super-eccentric planets, the periastron is fixed due to conservation of orbital angular momentum and the energy dissipated per orbit is constant, implying that the rate of change in semi-major axis a is a-dot {proportional_to}a{sup 1/2} and consequently the number distribution satisfies dN/d log a{proportional_to}a{sup 1/2}. If this formation process produces most hot Jupiters, Kepler should detect several super-eccentric migrating progenitors of hot Jupiters, allowing for a test of high-eccentricity migration scenarios.

  15. Lensless single-exposure super-resolved interferometric microscopy

    Science.gov (United States)

    Granero, Luis; Ferreira, Carlos; García, Javier; Micó, Vicente

    2013-04-01

    Single Exposure Super Resolved Interferometric Microscopy (SESRIM) has been recently proposed as a way to achieve one dimensional super resolved imaging in digital holographic microscopy. SESRIM uses Red-Green-Blue (RGB) multiplexing for illuminating the sample having different propagation angles for each one of the three illumination wavelengths and it has been experimentally validated considering color (A. Calabuig, V. Mico, J. Garcia, Z. Zalevsky, and C. Ferreira, "Single-exposure super-resolved interferometric microscopy by red-green-blue multiplexing," Opt. Lett. 36, 885-887, 2011) and monochrome (A. Calabuig, J. Garcia, C. Ferreira, Z. Zalevsky, and V. Mico, "Resolution improvement by single-exposure superresolved interferometric microscopy with a monochrome sensor," J. Opt. Soc. Am. A 28, 2346-2358, 2011) digital sensors for holographic recording. In this contribution, we will first review some of the characteristics of the previously reported SESRIM approaches and second, we will present preliminary results for the extension of SESRIM to the field of lensless holographic microscopy. Experimental results are reported validating this new kind of superresolution imaging method named as lensless SESRIM (L-SESRIM).

  16. An investigation of genital ulcers in Jackson, Mississippi, with use of a multiplex polymerase chain reaction assay: high prevalence of chancroid and human immunodeficiency virus infection.

    Science.gov (United States)

    Mertz, K J; Weiss, J B; Webb, R M; Levine, W C; Lewis, J S; Orle, K A; Totten, P A; Overbaugh, J; Morse, S A; Currier, M M; Fishbein, M; St Louis, M E

    1998-10-01

    In 1994, an apparent outbreak of atypical genital ulcers was noted by clinicians at the sexually transmitted disease clinic in Jackson, Mississippi. Of 143 patients with ulcers tested with a multiplex polymerase chain reaction (PCR) assay, 56 (39%) were positive for Haemophilus ducreyi, 44 (31%) for herpes simplex virus, and 27 (19%) for Treponema pallidum; 12 (8%) were positive for > 1 organism. Of 136 patients tested for human immunodeficiency virus (HIV) by serology, 14 (10%) were HIV-seropositive, compared with none of 200 patients without ulcers (P genital ulcers and HIV infection in this population highlights the urgency of preventing genital ulcers in the southern United States.

  17. Ultrasonic assisted rapid synthesis of high uniform super-paramagnetic microspheres with core-shell structure and robust magneto-chromatic ability

    Science.gov (United States)

    Zhang, Wenyan; Chen, Jiahua; Wang, Wei; Lu, GongXuan; Hao, Lingyun; Ni, Yaru; Lu, Chunhua; Xu, Zhongzi

    2017-03-01

    Super-paramagnetic core-shell microspheres were synthesized by ultrasonic assisted routine under low ultrasonic irradiation powers. Compared with conventional routine, ultrasonic effect could not only improve the uniformity of the core-shell structure of Fe3O4@SiO2, but shorten the synthesis time in large scale. Owing to their hydrophilicity and high surface charge, the Fe3O4@SiO2 microspheres could be dispersed well in distilled water to form homogeneous colloidal suspension. The suspensions have favorable magneto-chromatic ability that they sensitively exhibit brilliant colorful ribbons by magnetic attraction. The colorful ribbons, which distributed along the magnetic lines, make morphology of the magnetic fields become "visible" to naked eyed. Those colorful ribbons originate from strong magnetic interaction between the microspheres and magnetic fields. Furthermore, the magneto-chromatic performance is reversible as the colorful ribbons vanished rapidly with the removing of magnetic fields. The silica layer effectively enhanced the acid resistance and surface-oxidation resistance of theFe3O4@SiO2 microspheres, so they could exhibit stable magnetic nature and robust magneto-chromatic property in acid environment.

  18. A New Resource-Saving, High Chromium and Manganese Super Duplex Stainless Steels 29Cr-12Mn-2Ni-1Mo-xN

    Institute of Scientific and Technical Information of China (English)

    MA Zheng-huan; ZHAO Xiang-juan; GE Chang-sheng; DING Tie-suo; LI Jun; XIAO Xue-shan

    2011-01-01

    A new family of resource saving, high chromium and manganese super duplex stainless steels (DSSs), with a composition in mass percent, % of Cr 0.29, Mn 0.12, Ni 2.0, Mo i. 0, and N 0. 51-0. 68, has been developed by examining the effect of N on the microstructure, mechanical properties and corrosion properties. The results show that these alloys have a balanced ferrite-austenite relation. The austenite volume fraction decreases with the solution treatment temperature, but it increases with an increase in N content. The increases in nitrogen enhance the ultimate tensile strength (UTS) and reduce the ductility of the material slightly. The pitting corrosion potential increases first and then decreases with an increase in nitrogen content when the amount of N arrives to 0.68%. The yield stress and ultimate tensile strength of solution treated samples were more than 680 and 900 MPa, the elongation of experimental alloys are higher than 30%, respectively, what is more, the pitting potentials were beyond 1 100 mV.

  19. A design of novel type superconducting magnet for super-high field functional magnetic resonance imaging by using the harmonic analysis method of magnetic vector potentials

    Institute of Scientific and Technical Information of China (English)

    俎栋林; 郭华; 宋枭禹; 包尚联

    2002-01-01

    The approach of expanding the magnetic scalar potential in a series of Legendre polynomials is suitable for designing a conventional superconducting magnetic resonance imaging magnet of distributed solenoidal configuration. Whereas the approach of expanding the magnetic vector potential in associated Legendre harmonics is suitable for designing a single-solenoid magnet that has multiple tiers, in which each tier may have multiple layers with different winding lengths. A set of three equations to suppress some of the lowest higher-order harmonics is found. As an example, a 4T single-solenoid magnetic resonance imaging magnet with 4 × 6 layers of superconducting wires is de signed The degree of homogeneity in the 0.5m diameter sphere volume is better than 5.8 ppm. The same degree of homogeneity is retained after optimal integralization of turns in each correction layer. The ratio Bm/Bo in the single-solenoid magnet is 30%lower than that in the conventional six-solenoid magnet. This tolerates higher rated superconducting current in the coil. The Lorentz force of the coil in the single-solenoid system is also much lower than in the six-solenoid system. This novel type of magnet possesses significant advantage over conventional magnets, especially when used as a super-high field functional magnetic resonance imaging magnet.

  20. Identifying the source of super-high energetic electrons in the presence of pre-plasma in laser-matter interaction at relativistic intensities

    CERN Document Server

    Wu, D; Luan, S X; Yu, W

    2015-01-01

    The generation of super-high energetic electrons influenced by pre-plasma in relativistic intensity laser matter interaction is studied in a one-dimensional slab approximation with particle-in-cell simulations. Different pre-plasma scale-lengths of $1\\ \\mu\\text{m}$, $5\\ \\mu\\text{m}$, $10\\ \\mu\\text{m}$ and $15\\ \\mu\\text{m}$ are considered, showing an increase in both particle number and cut-off kinetic energy of energetic electrons with the increase of pre-plasma scale-length, and the obtained cut-off electron energies greatly exceeding the ponderomotive energies. A two-stage electron acceleration model is proposed to explain the underlying physics. The first stage is attributed to the synergetic acceleration by longitudinal electric field and laser pulse, with the efficiency depending on the pre-plasma scale-length. The fast electrons pre-accelerated in the first stage could build up an intense electrostatic potential with the potential energy several times as large of the initial electron kinetic energy. Par...

  1. Super-aligned carbon nanotube films with a thin metal coating as highly conductive and ultralight current collectors for lithium-ion batteries

    Science.gov (United States)

    Wang, Ke; Wu, Yang; Wu, Hengcai; Luo, Yufeng; Wang, Datao; Jiang, Kaili; Li, Qunqing; Li, Yadong; Fan, Shoushan; Wang, Jiaping

    2017-05-01

    Cross-stacked super-aligned carbon nanotube (SACNT) films are promising for use as current collectors in lithium-ion batteries because of their outstanding capability to decrease the weight and thickness of inactive material and strong adhesion to the electrodes. However, the relatively poor conductivity of SACNT films may limit their application to large-size electrodes or at high current rate. Herein, a facile approach is proposed to improve the conductivity of SACNT films by electron-beam deposition of a thin metal film on their surface. Such modification lowers the sheet resistance by three orders of magnitude while keeping the extremely small fraction of SACNT current collectors. The metal-coated SACNT films strongly inhibit polarization during the electrochemical reaction, resulting in improved cell performance compared with that of metal and uncoated CNT current collectors. The improvement in conductivity and cell performance achieved by this approach is so large that the effect of the increase of inactive material is overwhelmed, leading to increased gravimetric energy density.

  2. Identifying the source of super-high energetic electrons in the presence of pre-plasma in laser-matter interaction at relativistic intensities

    Science.gov (United States)

    Wu, D.; Krasheninnikov, S. I.; Luan, S. X.; Yu, W.

    2017-01-01

    The generation of super-high energetic electrons influenced by pre-plasma in relativistic intensity laser-matter interaction is studied in a one-dimensional slab approximation with particle-in-cell simulations. Different pre-plasma scale lengths and laser intensities are considered, showing an increase in both particle number and cut-off kinetic energy of electrons with the increase of pre-plasma scale length and laser intensity, the cut-off kinetic energy greatly exceeding the corresponding laser ponderomotive energy. A two-stage electron acceleration model is proposed to explain the underlying physics. The first stage is attributed to the synergetic acceleration by longitudinal electric field and counter-propagating laser pulses, and a scaling law is obtained with efficiency depending on the pre-plasma scale length and laser intensity. These electrons pre-accelerated in the first stage could build up an intense electrostatic potential barrier with maximal value several times as large as the initial electron kinetic energy. Some of the energetic electrons could be further accelerated by reflection off the electrostatic potential barrier, with their finial kinetic energies significantly higher than the values pre-accelerated in the first stage.

  3. A super-high throughput implementation of MD5 algorithm on FPGA%超高吞吐量MD5算法的FPGA实现

    Institute of Scientific and Technical Information of China (English)

    王臣; 袁焱

    2011-01-01

    In this paper, a novel architecture is proposed to increase the performance of MD5 computing in FPGA , which can achieve a super-high throughput. The scheme is based on full-pipeline for MD5 implementation. Moreover, FIFO cache structure is used for data storage and cooperative computing. The results of simulation and implementation show that the scheme can achieve the theoretical upper bound on throughput. And based on the same platform of FPGA, it outperforms the published scheme with best performance in terms of 77% higher throughput.%为了提高MD5算法在FPGA中实现的运算效率,使之达到超高的数据吞吐量,提出了一种新的全流水线架构,用于实现MD5算法.架构中使用了FIFO缓存存储数据,以配合流水线的运算.实验验证其达到了单个MD5运算单元运算吞吐量的理论上限,在相同芯片平台上,超过已发表的MD5运算模块最高吞吐量的77%.

  4. High dynamic range measurement of spectral responsivity and linearity of a radiation thermometer using a super-continuum laser and LEDs

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, Y. S.; Lee, D. H.; Park, C. W.; Park, S. N. [Korea Research Institute of Standards and Science 209 Gajeong-Ro, Yuseong-Gu, Daejon 305-340 (Korea, Republic of)

    2013-09-11

    To realize the temperature scale above the freezing point of silver according to the definition of ITS-90, the dynamic range of the spectral responsivity is one of the most important factors which limit its uncertainty. When the residual spectral response at both side bands of a spectral band is not negligible, a significant uncertainty can be caused by a low dynamic range of the spectral responsivity measurement. In general, incandescent lamps are used to measure the spectral responsivity and the linearity. The dynamic range of the spectral responsivity measurement is often limited by a trade-off with the desired spectral resolution, which is less than 6 decades. Nonlinearity is another limiting fact of uncertainties of the temperature scale. Tungsten lamps have disadvantage in the nonlinearity measurements in terms of adjustability of radiance level and spectral selectivity. We report spectral responsivity measurements of which the measurable dynamic range is enhanced 50 times after replacing a QTH lamp with a super continuum laser. We also present a spectrally selected linearity measurement over a wide dynamic range using high-brightness light emitting diode arrays to observe a slight saturation of linearity.

  5. Generation of warm dense matter and strongly coupled plasmas using the High Radiation on Materials facility at the CERN Super Proton Synchrotron

    CERN Document Server

    Tahir, N A; Brugger, M; Assmann, R; Shutov, A; Lomonosov, I V; Gryaznov, V; Piriz, A R; Udrea, S; Hoffmann, D H H; Fortov, V E; Deutsch, C

    2009-01-01

    A dedicated facility named High Radiation on Materials (HiRadMat) is being constructed at CERN to study the interaction of the 450 GeV protons generated by the Super Proton Synchrotron (SPS) with fixed solid targets of different materials. The main purpose of these future experiments is to study the generation and propagation of thermal shock waves in the target in order to assess the damage caused to the equipment, including collimators and absorbers, in case of an accident involving an uncontrolled release of the entire beam at a given point. Detailed numerical simulations of the beam-target interaction of several cases of interest have been carried out. In this paper we present simulations of the thermodynamic and the hydrodynamic response of a solid tungsten cylindrical target that is facially irradiated with the SPS beam with nominal parameters. These calculations have been carried out in two steps. First, the energy loss of the protons is calculated in the solid target using the FLUKA code (Fasso et al....

  6. Demonstration of Time Domain Multiplexed Readout for Magnetically Coupled Calorimeters

    Science.gov (United States)

    Porst, J.-P.; Adams, J. S.; Balvin, M.; Bandler, S.; Beyer, J.; Busch, S. E.; Drung, D.; Seidel, G. M.; Smith, S. J.; Stevenson, T. R.

    2012-01-01

    Magnetically coupled calorimeters (MCC) have extremely high potential for x-ray applications due to the inherent high energy resolution capability and being non-dissipative. Although very high energy-resolution has been demonstrated, until now there has been no demonstration of multiplexed read-out. We report on the first realization of a time domain multiplexed (TDM) read-out. While this has many similarities with TDM of transition-edge-sensors (TES), for MGGs the energy resolution is limited by the SQUID read-out noise and requires the well established scheme to be altered in order to minimize degradation due to noise aliasing effects. In cur approach, each pixel is read out by a single first stage SQUID (SQ1) that is operated in open loop. The outputs of the SQ1 s are low-pass filtered with an array of low cross-talk inductors, then fed into a single-stage SQUID TD multiplexer. The multiplexer is addressed from room temperature and read out through a single amplifier channel. We present results achieved with a new detector platform. Noise performance is presented and compared to expectations. We have demonstrated multiplexed X-ray spectroscopy at 5.9keV with delta_FWHM=10eV. In an optimized setup, we show it is possible to multiplex 32 detectors without significantly degrading the Intrinsic detector resolution.

  7. Numerical simulations on inter-page crosstalk characteristics in three-dimensional shift multiplexed self-referential holographic data storage

    Science.gov (United States)

    Eto, Taisuke; Takabayashi, Masanori; Okamoto, Atsushi; Bunsen, Masatoshi; Okamoto, Takashi

    2016-08-01

    A three-dimensional (3D) shift multiplexing technique is applied to increase the number of multiplexed datapages in self-referential holographic data storage (SR-HDS), enabling holographic recording with purely one-beam geometry. In the 3D shift multiplexing technique, the recording medium is shifted not only along the x- and y-axes but also along the z-axis to multiplex the datapages. Because the shift directions in the 3D shift multiplexing technique are expanded compared with the conventional 2D technique, the number of multiplexed datapages is expected to be increased. We numerically clarify the appropriate 3D recording layout of holograms in which the effect of the inter-page crosstalk can be reduced after showing that datapages are multiplexed on shifting the recording medium along the z-axis. The results show 3D shift multiplexing can effectively realize high-density SR-HDS when appropriately designed layouts are used.

  8. Portable Multiplex Pathogen Detector

    Energy Technology Data Exchange (ETDEWEB)

    Visuri, S; McBride, M T; Matthews, D; Rao, R

    2002-07-15

    Tumor marker concentrations in serum provide useful information regarding clinical stage and prognosis of cancer and can thus be used for presymptomatic diagnostic purposes. Currently, detection and identification of soluble analytes in biological fluids is conducted by methods including bioassays, ELISA, PCR, DNA chip or strip tests. While these technologies are generally sensitive and specific, they are time consuming, labor intensive and cannot be multiplexed. Our goal is to develop a simple, point-of-care, portable, liquid array-based immunoassay device capable of simultaneous detection of a variety of cancer markers. Here we describe the development of assays for the detection of Serum Prostate Specific Antigen, and Ovalbumin from a single sample. The multiplexed immunoassays utilize polystyrene microbeads. The beads are imbedded with precise ratios of red and orange fluorescent dyes yielding an array of 100 beads, each with a unique spectral address (Figure 1). Each bead can be coated with capture antibodies specific for a given antigen. After antigen capture, secondary antibodies sandwich the bound antigen and are indirectly labeled by the fluorescent reporter phycoerythrin (PE). Each optically encoded and fluorescently-labeled microbead is then individually interrogated. A red laser excites the dye molecules imbedded inside the bead and classifies the bead to its unique bead set, and a green laser quantifies the assay at the bead surface. This technology has been proven to be comparable to the ELISA in terms of sensitivity and specificity. We also describe the laser-based instrumentation used to acquire fluorescent bead images Following the assay, droplets of bead suspension containing a mixture of bead classes were deposited onto filters held in place by a disposable plexiglass device and the resultant arrays viewed under the fluorescent imaging setup. Using the appropriate filter sets to extract the necessary red, orange and green fluorescence from the

  9. On-chip noninterference angular momentum multiplexing of broadband light.

    Science.gov (United States)

    Ren, Haoran; Li, Xiangping; Zhang, Qiming; Gu, Min

    2016-05-13

    Angular momentum division has emerged as a physically orthogonal multiplexing method in high-capacity optical information technologies. However, the typical bulky elements used for information retrieval from the overall diffracted field, based on the interference method, impose a fundamental limit toward realizing on-chip multiplexing. We demonstrate noninterference angular momentum multiplexing by using a mode-sorting nanoring aperture with a chip-scale footprint as small as 4.2 micrometers by 4.2 micrometers, where nanoring slits exhibit a distinctive outcoupling efficiency on tightly confined plasmonic modes. The nonresonant mode-sorting sensitivity and scalability of our approach enable on-chip parallel multiplexing over a bandwidth of 150 nanometers in the visible wavelength range. The results offer the possibility of ultrahigh-capacity and miniaturized nanophotonic devices harnessing angular momentum division.

  10. Super-hybrid composites - An emerging structural material

    Science.gov (United States)

    Chamis, C. C.; Lark, R. F.; Sullivan, T. L.

    1975-01-01

    Specimens of super-hybrids and advanced fiber composites were subjected to extensive tests to determine their mechanical properties, including impact and thermal fatigue. The super-hybrids were fabricated by a procedure similar to that reported by Chamis et al., (1975). Super-hybrids subjected to 1000 cycles of thermal fatigue from -100 to 300 F retained over 90% of their longitudinal flexural strength and over 75% of their transverse flexural strength; their transverse flexural strength may be as high as 8 times that of a commercially supplied boron/1100-Al composite. The thin specimen Izod longitudinal impact resistance of the super-hybrids was twice that of the boron/110-Al material. Super-hybrids subjected to transverse tensile loads exhibited nonlinear stress-strain relationships. The experimentally determined initial membrane (in-plane) and bending elastic properties of super-hybrids were predicted adequately by linear laminate analysis.

  11. High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization.

    Science.gov (United States)

    Holden, Seamus J; Pengo, Thomas; Meibom, Karin L; Fernandez Fernandez, Carmen; Collier, Justine; Manley, Suliana

    2014-03-25

    We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of "Z-ring" organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.

  12. Recent advances in high performance poly(lactide): From ``green'' plasticization to super-tough materials via (reactive) compounding

    Science.gov (United States)

    Kfoury, Georgio; Raquez, Jean-Marie; Hassouna, Fatima; Odent, Jérémy; Toniazzo, Valérie; Ruch, David; Dubois, Philippe

    2013-12-01

    Due to its origin from renewable resources, its biodegradability, and recently, its industrial implementation at low costs, poly(lactide) (PLA) is considered as one of the most promising ecological, bio-sourced and biodegradable plastic materials to potentially and increasingly replace traditional petroleum derived polymers in many commodity and engineering applications. Beside its relatively high rigidity (high tensile strength and modulus compared with many common thermoplastics such as poly(ethylene terephthalate) (PET), high impact poly(styrene) (HIPS) and poly(propylene) (PP)), PLA suffers from an inherent brittleness, which can limit its applications especially where mechanical toughness such as plastic deformation at high impact rates or elongation is required. Therefore, the curve plotting stiffness vs. impact resistance and ductility must be shifted to higher values for PLA-based materials, while being preferably fully bio-based and biodegradable upon the application. This review aims to establish a state of the art focused on the recent progresses and preferably economically viable strategies developed in the literature for significantly improve the mechanical performances of PLA. A particular attention is given to plasticization as well as to impact resistance modification of PLA in the case of (reactive) blending PLA-based systems.

  13. Ultra-High Energy Cosmic Particles studies from space: super-EUSO, a possible next-generation experiment

    CERN Document Server

    Petrolini, Alessandro

    2009-01-01

    After the Pierre Auger Observatory, it is likely that space-based experiments might be required for next-generation studies of Ultra-High Energy Cosmic Particles. An overview of this challenging task is presented, emphasizing the main design issues, the criticalities and the intermediate steps required to make this challenging task a reality.

  14. High-efficiency super capacitors based on hetero-structured α-MnO{sub 2} nanorods

    Energy Technology Data Exchange (ETDEWEB)

    Ghouri, Zafar Khan [Department of BIN Fusion Technology, Chonbuk National University, Jeonju 561-756 (Korea, Republic of); Department of Organic materials and Fiber Engineering, Chonbuk National University, Jeonju 561-756 (Korea, Republic of); Shaheer Akhtar, M. [New & Renewable Energy Material Development Center (NewREC), Chonbuk National University, Jeonbuk (Korea, Republic of); Zahoor, Awan [Department of Chemical Engineering, NED University of Engineering & Technology, University Road, Karachi 75270 (Pakistan); Barakat, Nasser A.M., E-mail: nasser@jbnu.ac.kr [Department of Organic materials and Fiber Engineering, Chonbuk National University, Jeonju 561-756 (Korea, Republic of); Department of Chemical Engineering, Faculty of Engineering, El-Minia University, El-Minia (Egypt); Han, Weidong [Department of BIN Fusion Technology, Chonbuk National University, Jeonju 561-756 (Korea, Republic of); Park, Mira [Department of Organic materials and Fiber Engineering, Chonbuk National University, Jeonju 561-756 (Korea, Republic of); Pant, Bishweshwar; Saud, Prem Singh; Lee, Cho Hye [Department of BIN Fusion Technology, Chonbuk National University, Jeonju 561-756 (Korea, Republic of); Kim, Hak Yong, E-mail: khy@jbnu.ac.kr [Department of BIN Fusion Technology, Chonbuk National University, Jeonju 561-756 (Korea, Republic of)

    2015-09-05

    Highlights: • Hetero-structured α-MnO{sub 2} nanorods are prepared by a facile hydrothermal route. • It is applied as active electrode materials for supercapacitor. • A high specific capacitance of 298 Fg{sup −1} with a superior long term cyclic stability is achieved. • Supercapacitor shows high specific capacitance retention 94% after 1000 cycles. - Abstract: Hetero-structured manganese dioxide nanorods with α phase (α-MnO{sub 2}) were prepared by a facile hydrothermal route at low temperature. X-ray diffraction, scanning electron microscopy, transmission electron microscopy and nitrogen adsorption–desorption measurements were used to characterize the prepared hetero-structured α-MnO{sub 2} nanorods. Supercapacitive performance of the hetero-structured α-MnO{sub 2} nanomaterials as active electrode material was evaluated by cyclic voltammetry (CV) in alkaline medium. The MnO{sub 2} hetero-structure with 2 × 2 tunnels constructed from double chains of octahedral [MnO{sub 6}] structure yield a significantly high specific capacitance of 298 Fg{sup −1} at 5 mV s{sup −1} and demonstrated a superior long term cyclic stability, with specific capacitance retention about 94% after 1000 cycles. The superior supercapacitive performance of the hetero-structured α-MnO{sub 2} electrode is due to its high specific surface area and unique hierarchy architecture which facilitate fast electron and ion transport.

  15. Fuel Cell Power System and Equipment Bay for High Altitude, Super- Pressured, Powered Aerostat (HASPA) Operational Manual

    Science.gov (United States)

    1975-05-20

    Identify hy block number) Fuel cell High altitude Power system Aerostat f HASPA Manual .A8SRACt (Continue on reverse aide If rI coeeary and identify...Control Unit 37 VI. Pin Identification - Fuel Cell Control Unit 38 VIH . Pin Identification - Fuel Cell Auxiliary Data Unit 39 - VIII. HASPA - Equipment

  16. Recent advances in high performance poly(lactide): from "green" plasticization to super-tough materials via (reactive) compounding.

    Science.gov (United States)

    Kfoury, Georgio; Raquez, Jean-Marie; Hassouna, Fatima; Odent, Jérémy; Toniazzo, Valérie; Ruch, David; Dubois, Philippe

    2013-01-01

    Due to its origin from renewable resources, its biodegradability, and recently, its industrial implementation at low costs, poly(lactide) (PLA) is considered as one of the most promising ecological, bio-sourced and biodegradable plastic materials to potentially and increasingly replace traditional petroleum derived polymers in many commodity and engineering applications. Beside its relatively high rigidity [high tensile strength and modulus compared with many common thermoplastics such as poly(ethylene terephthalate) (PET), high impact poly(styrene) (HIPS) and poly(propylene) (PP)], PLA suffers from an inherent brittleness, which can limit its applications especially where mechanical toughness such as plastic deformation at high impact rates or elongation is required. Therefore, the curve plotting stiffness vs. impact resistance and ductility must be shifted to higher values for PLA-based materials, while being preferably fully bio-based and biodegradable upon the application. This review aims to establish a state of the art focused on the recent progresses and preferably economically viable strategies developed in the literature for significantly improve the mechanical performances of PLA. A particular attention is given to plasticization as well as to impact resistance modification of PLA in the case of (reactive) blending PLA-based systems.

  17. Recent advances in high performance poly(lactide: From green plasticization to super-tough materials via (reactive compounding

    Directory of Open Access Journals (Sweden)

    Georgio eKfoury

    2013-12-01

    Full Text Available Due to its origin from renewable resources, its biodegradability, and recently, its industrial implementation at low costs, poly(lactide (PLA is considered as one of the most promising ecological, bio-sourced and biodegradable plastic materials to potentially and increasingly replace traditional petroleum derived polymers in many commodity and engineering applications. Beside its relatively high rigidity (high tensile strength and modulus compared with many common thermoplastics such as poly(ethylene terephthalate (PET, high impact poly(styrene (HIPS and poly(propylene (PP, PLA suffers from an inherent brittleness, which can limit its applications especially where mechanical toughness such as plastic deformation at high impact rates or elongation is required. Therefore, the curve plotting stiffness vs. impact resistance and ductility must be shifted to higher values for PLA-based materials, while being preferably fully bio-based and biodegradable upon the application.This review aims to establish a state of the art focused on the recent progresses and preferably economically viable strategies developed in the literature for significantly improve the mechanical performances of PLA. A particular attention is given to plasticization as well as to impact resistance modification of PLA in the case of (reactive blending PLA-based systems.

  18. A 128 Multiplexing Factor Time-Domain SQUID Multiplexer

    Science.gov (United States)

    Prêle, D.; Voisin, F.; Piat, M.; Decourcelle, T.; Perbost, C.; Chapron, C.; Rambaud, D.; Maestre, S.; Marty, W.; Montier, L.

    2016-07-01

    A cryogenic 128:1 Time-Domain Multiplexer (TDM) has been developed for the readout of kilo-pixel Transition Edge Sensor (TES) arrays dedicated to the Q&U Bolometric Interferometer for Cosmology (QUBIC) instrument which aims to measure the B-mode polarization of the Cosmic Microwave Background. Superconducting QUantum Interference Devices (SQUIDs) are usually used to read out TESs. Moreover, SQUIDs are used to build TDM by biasing sequentially the SQUIDs connected together—one for each TES. In addition to this common technique which allows a typical 32 multiplexing factor, a cryogenic integrated circuit provides a 4:1 second multiplexing stage. This cryogenic integrated circuit is one of the original part of our TDM achieving an unprecedented 128 multiplexing factor. We present these two dimension TDM stages: topology of the SQUID multiplexer, operation of the cryogenic integrated circuit, and integration of the full system to read out a TES array dedicated to the QUBIC instrument. Flux-locked loop operation in multiplexed mode is also discussed.

  19. 超高层钢结构建筑施工工艺控制要点%The construction technology control key points of super high-rise steel structure buildings

    Institute of Scientific and Technical Information of China (English)

    郭锐; 刘刚彬

    2012-01-01

    This paper briefly introduced the present situation of super high-rise steel structure building,and elaborated in detail its construction technology,and combining with specific project example,this paper described the quality control key points of some key process in steel structure construction process,had significance to facilitate the construction level of future super high-rise steel structure buildings.%简要介绍了超高层钢结构建筑现状,对其施工工艺作了具体阐述,并结合具体工程实例,对钢结构工程施工过程中一些关键工序的质量控制要点进行了说明,对促进今后超高层建筑钢结构施工水平具有指导意义。

  20. Super-energy-saving dewatering method for high-specific-surface-area fuels by using dimethyl ether

    Energy Technology Data Exchange (ETDEWEB)

    Kanda, H. [Central Research Institute for Electric Power Industry, Kanagawa (Japan)

    2008-07-01

    There is a growing need for the economical dewatering of high-specific-surface-area fuels containing large amounts of water, such as coal and sewage sludge. The principle underlying conventional dewatering methods is evaporation of the water content by heating the fuels to a high temperature, but this approach consumes a considerable amount of energy. The Central Research Institute of the Electric Power Industry (CRIEPI) has developed a method for the extraction of this water through the use of dimethyl ether (DME), which liquefies at ordinary temperatures under the influence of a slight pressure. In this method, the water content in the fuel is extracted into the liquefied DME for separation from the fuel. After dewatering, the DME is depressurised, and subsequently vaporised, thereby leaving the separated water. Dewatering with an input energy of only 1109 kJ/kg water under ordinary conditions has been demonstrated theoretically.

  1. In-Situ Welding Carbon Nanotubes into a Porous Solid with Super-High Compressive Strength and Fatigue Resistance

    OpenAIRE

    2015-01-01

    Carbon nanotube (CNT) and graphene-based sponges and aerogels have an isotropic porous structure and their mechanical strength and stability are relatively lower. Here, we present a junction-welding approach to fabricate porous CNT solids in which all CNTs are coated and welded in situ by an amorphous carbon layer, forming an integral three-dimensional scaffold with fixed joints. The resulting CNT solids are robust, yet still highly porous and compressible, with compressive strengths up to 72...

  2. In-Situ Welding Carbon Nanotubes into a Porous Solid with Super-High Compressive Strength and Fatigue Resistance

    Science.gov (United States)

    Lin, Zhiqiang; Gui, Xuchun; Gan, Qiming; Chen, Wenjun; Cheng, Xiaoping; Liu, Ming; Zhu, Yuan; Yang, Yanbing; Cao, Anyuan; Tang, Zikang

    2015-06-01

    Carbon nanotube (CNT) and graphene-based sponges and aerogels have an isotropic porous structure and their mechanical strength and stability are relatively lower. Here, we present a junction-welding approach to fabricate porous CNT solids in which all CNTs are coated and welded in situ by an amorphous carbon layer, forming an integral three-dimensional scaffold with fixed joints. The resulting CNT solids are robust, yet still highly porous and compressible, with compressive strengths up to 72 MPa, flexural strengths up to 33 MPa, and fatigue resistance (recovery after 100,000 large-strain compression cycles at high frequency). Significant enhancement of mechanical properties is attributed to the welding-induced interconnection and reinforcement of structural units, and synergistic effects stemming from the core-shell microstructures consisting of a flexible CNT framework and a rigid amorphous carbon shell. Our results provide a simple and effective method to manufacture high-strength porous materials by nanoscale welding.

  3. Comparison of super-high-energy-propulsion-systems based on metallic hydrogen propellant for ES to LEO space transportation

    Science.gov (United States)

    Thierschmann, M.

    1990-01-01

    The application is studied of metallic H2 as a rocket propellant, which contains a specific energy of about 52 kcal/g in theory yielding a maximum specific impulse of 1700 s. With the convincing advantage of having a density 14 times that of conventional liquid H2/liquid O2 propellants, metallic H2 could satisfy the demands of advanced launch vehicle propulsion for the next millennium. Provided that there is an atomic metallic state of H2, and that this state is metastable at ambient pressure, which still is not proven, the results are given of the study of some important areas, which concern the production of metallic H2, the combustion, chamber cooling, and storage. The results show that the use of metallic H2 as rocket propellant could lead to revolutionary changes in space vehicle philosophy toward small size, small weight, and high performance single stage to orbit systems. The use of high metallic H2 mass fractions results in a dramatic reduction of required propellant volume, while gas temperatures in the combustion chamber exceed 5000 K. Furthermore, it follows, that H2 (liquid or slush) is the most favorable candidate as working fluid. Jet generated noise due to high exhaust velocities could be a problem.

  4. Raspberry Pi super cluster

    CERN Document Server

    Dennis, Andrew K

    2013-01-01

    This book follows a step-by-step, tutorial-based approach which will teach you how to develop your own super cluster using Raspberry Pi computers quickly and efficiently.Raspberry Pi Super Cluster is an introductory guide for those interested in experimenting with parallel computing at home. Aimed at Raspberry Pi enthusiasts, this book is a primer for getting your first cluster up and running.Basic knowledge of C or Java would be helpful but no prior knowledge of parallel computing is necessary.

  5. Chicken QTL mapping by multiplex PCR

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    To facilitate rapid determination of the chromosomal location of quantitative trait loci, the current approaches to gene mapping are improved using a multiplex PCR technique. The high-throughput linkage analysis method described here allows selection of 178 from 328 microsatellite markers through the multiplex PCR method combined with the semi-automatic fluorescence-labeled DNA analysis technology. Those polymorphism markers are distributed on 23 autosomes and one sex chromosome (chromosome Z), covering 3080cM genetic distance. The average marker density is 18cM, dispersed into 30 different sets. These selected polymorphism microsatellite markers segregate with the family members, following the Mendel's heritage laws, and are very useful for chicken linkage map analysis as well as for the research on some important economic quantitative characters of chicken.

  6. Magnetically Multiplexed Heating of Single Domain Nanoparticles

    CERN Document Server

    Christiansen, Michael G; Anikeeva, Polina

    2014-01-01

    Selective hysteretic heating of multiple collocated sets of single domain magnetic nanoparticles (SDMNPs) by alternating magnetic fields (AMFs) may offer a useful tool for biomedical applications. The possibility of magnetothermal multiplexing has not yet been realized, in part due to prevalent use of linear response theory to model SDMNP heating in AMFs. Predictive successes of dynamic hysteresis (DH), a more generalized model for heat dissipation by SDMNPs, are observed experimentally with detailed calorimetry measurements performed at varied AMF amplitudes and frequencies. The DH model suggests that specific driving conditions play an underappreciated role in determining optimal material selection strategies for high heat dissipation. Motivated by this observation, magnetothermal multiplexing is theoretically predicted and empirically demonstrated for the first time by selecting SDMNPs with properties that suggest optimal hysteretic heat dissipation at dissimilar AMF driving conditions. This form of multip...

  7. Percolation in real multiplex networks

    CERN Document Server

    Bianconi, Ginestra

    2016-01-01

    We present an exact mathematical framework able to describe site-percolation transitions in real multiplex networks. Specifically, we consider the average percolation diagram valid over an infinite number of random configurations where nodes are present in the system with given probability. The approach relies on the locally treelike ansatz, so that it is expected to accurately reproduce the true percolation diagram of sparse multiplex networks with negligible number of short loops. The performance of our theory is tested in social, biological, and transportation multiplex graphs. When compared against previously introduced methods, we observe improvements in the prediction of the percolation diagrams in all networks analyzed. Results from our method confirm previous claims about the robustness of real multiplex networks, in the sense that the average connectedness of the system does not exhibit any significant abrupt change as its individual components are randomly destroyed.

  8. Percolation in real multiplex networks

    Science.gov (United States)

    Bianconi, Ginestra; Radicchi, Filippo

    2016-12-01

    We present an exact mathematical framework able to describe site-percolation transitions in real multiplex networks. Specifically, we consider the average percolation diagram valid over an infinite number of random configurations where nodes are present in the system with given probability. The approach relies on the locally treelike ansatz, so that it is expected to accurately reproduce the true percolation diagram of sparse multiplex networks with negligible number of short loops. The performance of our theory is tested in social, biological, and transportation multiplex graphs. When compared against previously introduced methods, we observe improvements in the prediction of the percolation diagrams in all networks analyzed. Results from our method confirm previous claims about the robustness of real multiplex networks, in the sense that the average connectedness of the system does not exhibit any significant abrupt change as its individual components are randomly destroyed.

  9. Bond percolation on multiplex networks

    CERN Document Server

    Hackett, A; Gómez, S; Arenas, A; Gleeson, J P

    2015-01-01

    We present an analytical approach for bond percolation on multiplex networks and use it to determine the expected size of the giant connected component and the value of the critical bond occupation probability in these networks. We advocate the relevance of these tools to the modeling of multilayer robustness and contribute to the debate on whether any benefit is to be yielded from studying a full multiplex structure as opposed to its monoplex projection, especially in the seemingly irrelevant case of a bond occupation probability that does not depend on the layer. Although we find that in many cases the predictions of our theory for multiplex networks coincide with previously derived results for monoplex networks, we also uncover the remarkable result that for a certain class of multiplex networks, well described by our theory, new critical phenomena occur as multiple percolation phase transitions are present. We provide an instance of this phenomenon in a multipex network constructed from London rail and Eu...

  10. 不确定欠驱动系统的高阶自适应Super-Twisting滑模控制%High-Order Adaptive Super-Twisting Sliding Mode Control for Uncertain Underactuated Systems

    Institute of Scientific and Technical Information of China (English)

    杨兴明; 高银平

    2014-01-01

    为实现一类不确定欠驱动系统在未知干扰情况下的鲁棒控制,针对传统滑模控制中存在的抖振问题,提出一种基于二次型Lyapunov函数的二阶Super-Twisting自适应滑模控制策略。首先,控制器的不连续项采用二阶Super-Twisting算法,将不连续控制作用在滑模量的二阶导数。然后,针对滑模面受不确定干扰影响的情况,为调节参数设计一种自适应律方法,该方法不受传统二阶滑模控制中干扰项的一阶导数边界已知的条件限制,保证滑模面在有干扰情况下的收敛,削弱控制器输入的抖振现象。最后,以两轮自平衡车为实验对象验证该方法,并与传统滑模及普通二阶滑模方法做仿真对比。仿真结果表明文中所提的二阶自适应滑模控制方法在控制效果和降低抖振方面表现更优。%To achieve good robustness against disturbances for a class of uncertain underactuated systems, a second-order adaptive sliding mode control method is proposed based on quadratic Lyapunov function to reduce the inherent chattering of conventional sliding mode control ( SMC ) . Firstly, a second-order super-twisting algorithm is used by the discontinuous part of controller, which acts on the second-order derivative of sliding mode variables. Secondly, as for the effects of unknown disturbances on sliding mode surface, an adaptive law is designed to adjust the parameters. This method eliminates the restriction of the first derivative of disturbances boundary being known in the traditional second-order sliding mode control, which not only keeps convergence of sliding mode surface but also reduces chattering. Finally, a two-wheeled self-balancing cart is used to test the proposed approach. The simulation results show that compared with conventional SMC and ordinary second-order SMC, the proposed method outperforms the above methods on effectiveness and reducing chattering.

  11. The optimization of super-high resolution frequency measurement techniques based on phase quantization regularities between any frequencies.

    Science.gov (United States)

    Li, Zhiqi; Zhou, Wei; Zhou, Hui; Zhang, Xueping; Zhao, Jie

    2013-02-01

    Step phase quantization regularity between different nominal frequency signals is introduced in this paper. Based on this regularity, an optimized high resolution frequency measurement technique is presented. The key features and issues of phase quantization characteristics and measurements are described. Based on the relationship between the same or multiple nominal signals with a certain differences, the resolution of frequency measurements is developed and the range is widened. Several measurement results are provided to support the concepts with experimental evidence. The resolution of frequency measurement can reach 10(-12) (s(-1)) over a wide range or higher for specific frequency signals.

  12. Super-resolution imaging of Escherichia coli nucleoids reveals highly structured and asymmetric segregation during fast growth.

    Science.gov (United States)

    Spahn, Christoph; Endesfelder, Ulrike; Heilemann, Mike

    2014-03-01

    Bacterial replication and chromosome segregation are highly organized both in space and in time. However, spatial analysis is hampered by the resolution limit of conventional fluorescence microscopy. In this study, we incubate rapidly-growing Escherichia coli with 5-ethynyl-2'-deoxyuridine (EdU), label the resulting EdU-DNA with photoswitchable fluorophores, and image incorporated molecules with an average experimental precision of 13 nm. During the segregation process, nucleoids develop highly-defined and cell-cycle dependent hetero-structures, which contain discrete DNA fibers with diameters far below the diffraction limit. Strikingly, these structures appear temporally shifted between sister chromosomes, an asymmetry which accumulates for ongoing replication rounds. Moreover, nucleoid positioning and expansion along the bacterial length axis fit into an elongation-mediated segregation model in fast growing E. coli cultures. This is supported by close proximity of the nucleoids to the bacterial plasma membrane, the nature of the observed hetero-structures and recently found interactions of membrane-associated proteins with DNA.

  13. Microgels for multiplex and direct fluorescence detection

    Science.gov (United States)

    Causa, Filippo; Aliberti, Anna; Cusano, Angela M.; Battista, Edmondo; Netti, Paolo A.

    2015-05-01

    Blood borne oligonucleotides fragments contain useful clinical information whose detection and monitoring represent the new frontier in liquid biopsy as they can transform the current diagnosis procedure. For instance, recent studies have identified a new class of circulating biomarkers such as s miRNAs, and demonstrated that changes in their concentration are closely associated with the development of cancer and other pathologies. However, direct detection of miRNAs in body fluids is particularly challenging and demands high sensitivity -concentration range between atto to femtomolarspecificity, and multiplexing Here we report on engineered multifunctional microgels and innovative probe design for a direct and multiplex detection of relevant clinical miRNAs in fluorescence by single particle assay. Polyethyleneglycol-based microgels have a coreshell architecture with two spectrally encoded fluorescent dyes for multiplex analyses and are endowed with fluorescent probes for miRNA detection. Encoding and detection fluorescence signals are distinguishable by not overlapping emission spectra. Tuneable fluorescence probe conjugation and corresponding emission confinement on single microgel allows for enhanced target detection. Such suspension array has indeed high selectivity and sensitivity with a detection limit of 10-15 M and a dynamic range from 10-9 to 10-15 M. We believe that sensitivity in the fM concentration range, signal background minimization, multiplexed capability and direct measurement of such microgels will translate into diagnostic benefits opening up new roots toward liquid biopsy in the context of point-of-care testing through an easy and fast detection of sensitive diagnostic biomarkers directly in serum.

  14. Assessment of the impact of HTSCs on superconducting fault-current limiters. [High Temperature SuperConductors (HTSCs)

    Energy Technology Data Exchange (ETDEWEB)

    Giese, R.F. (Argonne National Lab., IL (United States)); Runde, M. (Energiforsyningens Forskningsinstitutt A/S, Trondheim (Norway))

    1993-01-01

    The possible impact of nitrogen-cooled superconductors on the desip and cost of superconducting fault-current limiters is assessed by considering the technical specifications such devices must meet and by comparing material properties of 77-K and 4-K superconductors. The main advantages of operating superconductors at 77 K are that the refrigeration operating cost is reduced by a factor of up to 25 and the refrigeration capital cost is reduced by a factor of up to 10. The heat capacity is several orders of magnitude Larger at 77 K and at 4 K. This phenomenon increases conductor stability against flux jumps but makes switching from the superconducting to the normal state slow and difficult. Therefore, a high critical current density, probably at least 10[sup 5] A/cm[sup 2], is required.

  15. Super-miniature multi-hot-film probe for sub-Kolmogorov resolution in high-Re turbulence

    Science.gov (United States)

    Borisenkov, Y.; Kholmyansky, M.; Krylov, S.; Liberzon, A.; Tsinober, A.

    2011-12-01

    The work reported here is motivated by the discovery of far more important role played by the sub-Kolmogorov scales than commonly believed. The first part is devoted to an overview of main results and issues that prompted the present developments. The emphasis is made on a number of manifestations of nonlocal nature of turbulence involving direct and bidirectional coupling of conventionally-defined inertial and dissipative ranges showing (a) that both concepts are ill-posed and (b) that further progress requires sub-Kolmogorov resolution. The second part contains a presentation on design, manufacturing and tests in laboratory of a micro-hot-film sensor using modern micro-fabrication technologies, as a basis for a probe of much smaller scale than available today with access to quantities like vorticity and strain at sub-Kolmogorov scales in high-Reynolds-number flows.

  16. Development of a super high-rate Anammox reactor and in situ analysis of biofilm structure and function.

    Science.gov (United States)

    Tsushima, Ikuo; Ogasawara, Yuji; Shimokawa, Masaki; Kindaichi, Tomonori; Okabe, Satoshi

    2007-01-01

    The anaerobic ammonium oxidation (Anammox) process is a new efficient and cost effective method of ammonium removal from wastewater. Under strictly anoxic condition, ammonium is directly oxidised with nitrite as electron acceptor to dinitrogen gas. However, it is extremely difficult to cultivate Anammox bacteria due to their low growth rate. This suggests that a rapid and efficient start-up of Anammox process is the key to practical applications. To screen appropriate seeding sludge with high Anammox potential, a real-time quantitative PCR assay with newly designed primers has been developed. Thereafter, the seeding sludge with high abundance of Anammox bacteria (1.7 x 10(8) copies/mg-dry weight) was selected and inoculated into an upflow anaerobic biofilters (UABs). The UABs were operated for more than 1 year and the highest nitrogen removal rate of 24.0 kg-N m-3 day(-1) was attained. In addition, the ecophysiology of Anammox bacteria (spatial distribution and in situ activity) in biofilms was analysed by combining a full-cycle 16S rRNA approach and microelectrodes. The microelectrode measurement clearly revealed that a successive vertical zonation of the partial nitrification (NH4+ to NO2-), Anammox reaction and denitrification was developed in the biofilm in the UAB. This result agreed with the spatial distribution of corresponding bacterial populations in the biofilm. We linked the micro-scale information (i.e. single cell and/or biofilm levels) with the macro-scale information (i.e. the reactor level) to understand the details of Anammox reaction occurring in the UABs.

  17. Simultaneous identification of three highly pathogenic Eimeria species in rabbits using a multiplex PCR diagnostic assay based on ITS1-5.8S rRNA-ITS2 fragments.

    Science.gov (United States)

    Yan, Wenchao; Wang, Wenlong; Wang, Tianqi; Suo, Xun; Qian, Weifeng; Wang, Shuai; Fan, Di

    2013-03-31

    Eimeria stiedai, E. intestinalis, and E. flavescens are highly pathogenic in rabbits, especially rabbits younger than 3 months. In this study, the complete ITS1-5.8S rRNA-ITS2 sequences of six rabbit Eimeria species, E. stiedai, E. intestinalis, E. flavescens, E. media, E. magna, and E. irresidua, were cloned with universal primers for the genus Eimeria and genomic DNA of LY and KF isolates as templates. These results revealed that both ITS1 and ITS2 sequences were specific to each Eimeria species in rabbits. A specific and sensitive multiplex PCR diagnostic assay based on polymorphic sites of ITS1 and ITS2 was developed and used to identify the three highly pathogenic species from rabbits, E. stiedai, E. intestinalis, and E. flavescens. Our findings provide a powerful tool for the clinical differentiation of highly pathogenic Eimeria species in rabbits and the study of the population genetics of rabbit coccidia.

  18. All-Optical Ultra-High-Speed OFDM to Nyquist-WDM Conversion Based on Complete Optical Fourier Transformation

    DEFF Research Database (Denmark)

    Guan, Pengyu; Røge, Kasper Meldgaard; Mulvad, Hans Christian Hansen

    2016-01-01

    We propose a novel all-optical ultra-high-speed orthogonal frequency-division multiplexing (OFDM) to Nyquist wavelength-division multiplexing (Nyquist-WDM) conversion scheme, achieved by exchanging the temporal and spectral profiles using a complete optical Fourier transformation (OFT). This scheme...... enables high-speed OFDM to Nyquist-WDM conversion without complex optical/electrical/optical conversion. The all-optical OFDM transmitter is based on the generation of OFDM symbols with a low duty cycle by rectangular temporal gating, which in combination with optical time-division multiplexing yields...... a higher symbol-rate OFDM signal. In the receiver, the converted Nyquist-WDM super-channel is WDM demultiplexed into individual Nyquist-WDM channels using a rectangular optical bandpass filter, followed by optical sampling at the intersymbol-interference free point. In the experimental demonstration...

  19. Front-end Multiplexing - applied to SQUID multiplexing : Athena X-IFU and QUBIC experiments

    CERN Document Server

    Prêle, Damien

    2015-01-01

    As we have seen for digital camera market and a sensor resolution increasing to "megapixels", all the scientific and high-tech imagers (whatever the wave length - from radio to X-ray range) tends also to always increases the pixels number. So the constraints on front-end signals transmission increase too. An almost unavoidable solution to simplify integration of large arrays of pixels is front-end multiplexing. Moreover, "simple" and "efficient" techniques allow integration of read-out multiplexers in the focal plane itself. For instance, CCD (Charge Coupled Device) technology has boost number of pixels in digital camera. Indeed, this is exactly a planar technology which integrates both the sensors and a front-end multiplexed readout. In this context, front-end multiplexing techniques will be discussed for a better understanding of their advantages and their limits. Finally, the cases of astronomical instruments in the millimeter and in the X-ray ranges using SQUID (Superconducting QUantum Interference Device...

  20. High-Resolution Imaging of Molecular Gas and Dust in the Antennae (NGC 4038/39) Super Giant Molecular Complexes

    CERN Document Server

    Wilson, C D; Madden, S C; Charmandaris, V

    2000-01-01

    We present new aperture synthesis CO maps of the Antennae (NGC 4038/39) obtained with the Caltech Millimeter Array. These sensitive images show molecular emission associated with the two nuclei and a partial ring of star formation to the west of NGC 4038, as well as revealing the large extent of the extra-nuclear region of star formation (the ``overlap region''), which dominates the CO emission from this system. The largest molecular complexes have masses of 3-6x10^8 M_sun, typically an order of magnitude larger than the largest structures seen to date in more quiescent galaxy disks. The extremely red luminous star clusters identified previously with HST are well-correlated with the CO emission, which supports the conclusion that they are highly embedded young objects rather than old globular clusters. There is an excellent correlation between the CO emission and the 15 micron emission seen with ISO, particularly for the brightest regions. The most massive complexes in the overlap region have similar [NeIII]/...