WorldWideScience

Sample records for sung kyu lim

  1. Mishima jo ha kyu

    Directory of Open Access Journals (Sweden)

    Matteo Casari

    2012-01-01

    Full Text Available Abstract – IT La grandezza artistica di Mishima si è espressa attraverso una eterogenea gamma di linguaggi tra i quali il teatro – nella drammaturgia ma anche nella saggistica, regia, recitazione e direzione di compagnia – ha avuto un ruolo di primo piano. Al pari di un uomo di scena Mishima inaugura, a partire dagli anni ’50, un processo di attenzione al proprio corpo come possibile, anzi necessario, veicolo di estrinsecazione etico-estetica tanto da poter istituire l’equazione corpo-teatro quale snodo profondo del suo processo creativo. Il corpo sognato e ottenuto da Mishima attraverso l’addestramento nel kendo, in altre arti marziali e nel body building si conformava ad un ideale estetico di matrice greco-classica assai lontano dal corpo teatrale nipponico. Il corpo come luogo di elaborazione e strumento di espressione autentica da realizzare con impegno, però, lo legano profondamente alle esperienze nascenti – tra gli anni ’50 e ’60 – delle avanguardie teatrali giapponesi. La metafora teatrale è spesso usata nella lettura critica del Mishima uomo e artista con accezione deteriore: un personaggio che dà spettacolo di sé con ripetute provocazioni tra le quali il suicidio del 25 novembre 1970 non sarebbe che l’esempio ultimo e più estremo. La costruzione di sé come personaggio, invece, sembrerebbe corrispondere ad una ben più profonda e meditata necessità di comporre la propria vita in una sapiente messa in scena di classica perfezione: i principi della scansione ritmico formale del jo ha kyu, pilastro teorico del teatro no codificato da Zeami tra XIV e XV secolo, offrono un valido modello di riferimento. Abstract – EN Mishima’s artistic greatness has been expressed through a diverse range of languages among wich theater – in dramaturgy as well as in written essays, as a director, performer and in company direction – played a central role. As a true front-man, Mishima – starting from the 50’s

  2. THE SWORD KYU-GUNTO IN JAPANESE HISTORY AND CULTURE (1875–1934

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    Alexander Victorovich Tsokur

    2015-01-01

    Full Text Available The article describes some reasons for the appearance of the sword Kyu-Gunto in the Japanese army in 1875, the main characteristics of the sword, the elements of European influence, and causes of transition to a new model in 1934. We consider special features of the sword KyuGunto depending on officer’s rank and branch of the armed forces. The importance of this problem is determined not only by the fighting qualities and purpose of the sword, but also by its ritual and even mystical meaning in Japanese society. Therefore, the evolution of the sword should be studied not only in terms of weapon’s history, but also in the context of cultural history. Sometimes the power of tradition impedes military and technological progress, but it also was a source of strength and resistance of Japanese forces in situations which their opponents considered as hopeless. The problem of comparative efficiency of bladed weapons of Japan and its opponents remains unexplored. On the eve of the World War II, the sword was not only a fighting weapon, it had a status value for the officers – it remained in service not only in infantry, but even in the navy and air forces, where it was impossible to use a sword in a battle.

  3. 从LIMS到Lims Solution%From LIMS to Lims Solution

    Institute of Scientific and Technical Information of China (English)

    杨海鹰; 沈彤; 陆婉珍

    2002-01-01

    探讨了实验室信息管理系统(Laboratory Information Management Systems,LIMS)应用推广的问题,提出了Lims Solution(实验室信息管理解决方案)的概念,并对LIMSLims Solution两者的差异及应用特点进行了讨论,认为后者是LIMS技术推广应用的最佳模式.

  4. Perception of Sung Speech in Bimodal Cochlear Implant Users

    Science.gov (United States)

    Galvin, John J.; Fu, Qian-Jie

    2016-01-01

    Combined use of a hearing aid (HA) and cochlear implant (CI) has been shown to improve CI users’ speech and music performance. However, different hearing devices, test stimuli, and listening tasks may interact and obscure bimodal benefits. In this study, speech and music perception were measured in bimodal listeners for CI-only, HA-only, and CI + HA conditions, using the Sung Speech Corpus, a database of monosyllabic words produced at different fundamental frequencies. Sentence recognition was measured using sung speech in which pitch was held constant or varied across words, as well as for spoken speech. Melodic contour identification (MCI) was measured using sung speech in which the words were held constant or varied across notes. Results showed that sentence recognition was poorer with sung speech relative to spoken, with little difference between sung speech with a constant or variable pitch; mean performance was better with CI-only relative to HA-only, and best with CI + HA. MCI performance was better with constant words versus variable words; mean performance was better with HA-only than with CI-only and was best with CI + HA. Relative to CI-only, a strong bimodal benefit was observed for speech and music perception. Relative to the better ear, bimodal benefits remained strong for sentence recognition but were marginal for MCI. While variations in pitch and timbre may negatively affect CI users’ speech and music perception, bimodal listening may partially compensate for these deficits. PMID:27837051

  5. A Comprehensive Trainable Error Model for Sung Music Queries

    CERN Document Server

    Birmingham, W P; 10.1613/jair.1334

    2011-01-01

    We propose a model for errors in sung queries, a variant of the hidden Markov model (HMM). This is a solution to the problem of identifying the degree of similarity between a (typically error-laden) sung query and a potential target in a database of musical works, an important problem in the field of music information retrieval. Similarity metrics are a critical component of query-by-humming (QBH) applications which search audio and multimedia databases for strong matches to oral queries. Our model comprehensively expresses the types of error or variation between target and query: cumulative and non-cumulative local errors, transposition, tempo and tempo changes, insertions, deletions and modulation. The model is not only expressive, but automatically trainable, or able to learn and generalize from query examples. We present results of simulations, designed to assess the discriminatory potential of the model, and tests with real sung queries, to demonstrate relevance to real-world applications.

  6. Melodic contour identification and sentence recognition using sung speech.

    Science.gov (United States)

    Crew, Joseph D; Galvin, John J; Fu, Qian-Jie

    2015-09-01

    For bimodal cochlear implant users, acoustic and electric hearing has been shown to contribute differently to speech and music perception. However, differences in test paradigms and stimuli in speech and music testing can make it difficult to assess the relative contributions of each device. To address these concerns, the Sung Speech Corpus (SSC) was created. The SSC contains 50 monosyllable words sung over an octave range and can be used to test both speech and music perception using the same stimuli. Here SSC data are presented with normal hearing listeners and any advantage of musicianship is examined.

  7. Hemispheric specialization for linguistic processing of sung speech.

    Science.gov (United States)

    Yelle, Serena K; Grimshaw, Gina M

    2009-02-01

    The two hemispheres of the brain play complementary roles in song perception, with the left hemisphere specialized for processing the linguistic aspects of song and the right hemisphere specialized for the processing of melody. However, very little is known about how language and melody interact. The present study tested the hypothesis that right hemisphere linguistic processing would be facilitated by the presence of melody. In a dichotic listening paradigm, participants (8 men, 43 women) performed a linguistic task while listening to spoken or sung speech. Contrary to the hypothesis, left hemisphere specialization for linguistic processing was identical whether the sentences were spoken or sung.

  8. A Perl toolkit for LIMS development

    Directory of Open Access Journals (Sweden)

    Jacobs Ian J

    2008-03-01

    Full Text Available Abstract Background High throughput laboratory techniques generate huge quantities of scientific data. Laboratory Information Management Systems (LIMS are a necessary requirement, dealing with sample tracking, data storage and data reporting. Commercial LIMS solutions are available, but these can be both costly and overly complex for the task. The development of bespoke LIMS solutions offers a number of advantages, including the flexibility to fulfil all a laboratory's requirements at a fraction of the price of a commercial system. The programming language Perl is a perfect development solution for LIMS applications because of Perl's powerful but simple to use database and web interaction, it is also well known for enabling rapid application development and deployment, and boasts a very active and helpful developer community. The development of an in house LIMS from scratch however can take considerable time and resources, so programming tools that enable the rapid development of LIMS applications are essential but there are currently no LIMS development tools for Perl. Results We have developed ArrayPipeline, a Perl toolkit providing object oriented methods that facilitate the rapid development of bespoke LIMS applications. The toolkit includes Perl objects that encapsulate key components of a LIMS, providing methods for creating interactive web pages, interacting with databases, error tracking and reporting, and user and session management. The MT_Plate object provides methods for manipulation and management of microtitre plates, while a given LIMS can be encapsulated by extension of the core modules, providing system specific methods for database interaction and web page management. Conclusion This important addition to the Perl developer's library will make the development of in house LIMS applications quicker and easier encouraging laboratories to create bespoke LIMS applications to meet their specific data management requirements.

  9. Enhanced Neonatal Brain Responses To Sung Streams Predict Vocabulary Outcomes By Age 18 Months.

    Science.gov (United States)

    François, Clément; Teixidó, Maria; Takerkart, Sylvain; Agut, Thaïs; Bosch, Laura; Rodriguez-Fornells, Antoni

    2017-09-29

    Words and melodies are some of the basic elements infants are able to extract early in life from the auditory input. Whether melodic cues contained in songs can facilitate word-form extraction immediately after birth remained unexplored. Here, we provided converging neural and computational evidence of the early benefit of melodies for language acquisition. Twenty-eight neonates were tested on their ability to extract word-forms from continuous flows of sung and spoken syllabic sequences. We found different brain dynamics for sung and spoken streams and observed successful detection of word-form violations in the sung condition only. Furthermore, neonatal brain responses for sung streams predicted expressive vocabulary at 18 months as demonstrated by multiple regression and cross-validation analyses. These findings suggest that early neural individual differences in prosodic speech processing might be a good indicator of later language outcomes and could be considered as a relevant factor in the development of infants' language skills.

  10. The LIM class homeobox gene lim5: implied role in CNS patterning in Xenopus and zebrafish.

    Science.gov (United States)

    Toyama, R; Curtiss, P E; Otani, H; Kimura, M; Dawid, I B; Taira, M

    1995-08-01

    LIM homeobox genes are characterized by encoding proteins in which two cysteine-rich LIM domains are associated with a homeodomain. We report the isolation of a gene, named Xlim-5 in Xenopus and lim5 in the zebrafish, that is highly similar in sequence but quite distinct in expression pattern from the previously described Xlim-1/lim1 gene. In both species studied the lim5 gene is expressed in the entire ectoderm in the early gastrula embryo. The Xlim-5 gene is activated in a cell autonomous manner in ectodermal cells, and this activation is suppressed by the mesoderm inducer activin. During neurulation, expression of the lim5 gene in both the frog and fish embryo is rapidly restricted to an anterior region in the developing neural plate/keel. In the 2-day Xenopus and 24-hr zebrafish embryo, this region becomes more sharply defined, forming a strongly lim5-expressing domain in the diencephalon anterior to the midbrain-forebrain boundary. In addition, regions of less intense lim5 expression are seen in the zebrafish embryo in parts of the telencephalon, in the anterior diencephalon coincident with the postoptic commissure, and in restricted regions of the midbrain, hindbrain, and spinal cord. Expression in ventral forebrain is abolished from the 5-somite stage onward in cyclops mutant fish. These results imply a role for lim5 in the patterning of the nervous system, in particular in the early specification of the diencephalon.

  11. Learning sung lyrics aids retention in normal ageing and Alzheimer's disease.

    Science.gov (United States)

    Moussard, Aline; Bigand, Emmanuel; Belleville, Sylvie; Peretz, Isabelle

    2014-01-01

    Previous studies have suggested that presenting to-be-memorised lyrics in a singing mode, instead of a speaking mode, may facilitate learning and retention in normal adults. In this study, seven healthy older adults and eight participants with mild Alzheimer's disease (AD) learned and memorised lyrics that were either sung or spoken. We measured the percentage of words recalled from these lyrics immediately and after 10 minutes. Moreover, in AD participants, we tested the effect of successive learning episodes for one spoken and one sung excerpt, as well as long-term retention after a four week delay. Sung conditions did not influence lyrics recall in immediate recall but increased delayed recall for both groups. In AD, learning slopes for sung and spoken lyrics did not show a significant difference across successive learning episodes. However, sung lyrics showed a slight advantage over spoken ones after a four week delay. These results suggest that singing may increase the load of initial learning but improve long-term retention of newly acquired verbal information. We further propose some recommendations on how to maximise these effects and make them relevant for therapeutic applications.

  12. Laboratory automation and LIMS in forensics

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Hansen, Anders Johannes; Morling, Niels

    2013-01-01

    Implementation of laboratory automation and LIMS in a forensic laboratory enables the laboratory, to standardize sample processing. Automated liquid handlers can increase throughput and eliminate manual repetitive pipetting operations, known to result in occupational injuries to the technical staff....... Furthermore, implementation of automated liquid handlers reduces the risk of sample misplacement. A LIMS can efficiently control the sample flow through the laboratory and manage the results of the conducted tests for each sample. Integration of automated liquid handlers with a LIMS provides the laboratory...... with the tools required for setting up automated production lines of complex laboratory processes and monitoring the whole process and the results. Combined, this enables processing of a large number of samples. Selection of the best automated solution for an individual laboratory should be based on user...

  13. LIMS use in laboratory data management

    Directory of Open Access Journals (Sweden)

    Muntean Edward

    2008-12-01

    Full Text Available Laboratory data management is a complex process involving both people and information in order toacquire, organize, store and distribute information. Information conversion to knowledge requires the applicationof many human qualities such as experience, intelligence, intuition and talent; it is not enough to simply acquireand own data, being necessary to have a proper understanding of the data structure and the required qualities toapply a suitable management system. Modern laboratories produce large amounts of data; with the continuousdevelopment of new technologies, both the quality and quantity of information is increasing, causing oftensignificant problems and new methods are needed to manage this situation. Laboratory Information ManagementSystems (LIMS provides a way of automating part of the laboratory system. As the primary function of mostlaboratories is to provide validated information under some time constraints and based on that information,allowing customers to make decisions, LIMS can save considerable amounts of time and dramatically improvethe level of data access for all individuals involved in a given project; the sooner the user is notified of aproblem, the sooner that problem can be fixed and the less the solution will cost. Several of the main benefitsidentified from current users of LIMS are: analytical data can be obtained instantly being also easily accessibleonline; large amounts of data can be stored without the need for traditional archiving; improvement of dataquality as all the analytical instruments are integrated; automated tracking and management; automated customerreports; automated quality control. All these benefits can lead finally to a general improvement of laboratoryefficiency. Despite LIMS have been used for more than twenty years, they still remain difficult to implementsuccessfully. This paper provides a brief description of some of the existing technologies available to date,detailing the benefits that

  14. Expression patterns of the murine LIM class homeobox gene lim1 in the developing brain and excretory system.

    Science.gov (United States)

    Fujii, T; Pichel, J G; Taira, M; Toyama, R; Dawid, I B; Westphal, H

    1994-01-01

    We report the cloning, sequence analysis, and developmental expression pattern of lim1, a member of the LIM class homeobox gene family in the mouse. lim1 cDNA encodes a predicted 406 amino acid protein that is 93% identical with the product of the Xenopus LIM class homeobox gene Xlim1. We have characterized lim1 expression from day 8.5 post coitum onward. Northern blot analysis of RNA transcripts indicates that lim1 is expressed both during embryogenesis and in the adult brain. Analysis by whole-mount and section in situ hybridization shows lim1 expression in the central nervous system from the telencephalon through the spinal cord and in the developing excretory system including pronephric region, mesonephros, nephric duct, and metanephros. In the metanephros, lim1 is strongly expressed in renal vesicles and S-shaped bodies, and transcripts are also detected in the ureteric branches.

  15. Galaxy LIMS for next-generation sequencing

    NARCIS (Netherlands)

    Scholtalbers, J.; Rossler, J.; Sorn, P.; Graaf, J. de; Boisguerin, V.; Castle, J.; Sahin, U.

    2013-01-01

    SUMMARY: We have developed a laboratory information management system (LIMS) for a next-generation sequencing (NGS) laboratory within the existing Galaxy platform. The system provides lab technicians standard and customizable sample information forms, barcoded submission forms, tracking of input sam

  16. Comparing sea ice, hydrography and circulation between NEMO3.6 LIM3 and LIM2

    Science.gov (United States)

    Uotila, Petteri; Iovino, Doroteaciro; Vancoppenolle, Martin; Lensu, Mikko; Rousset, Clement

    2017-03-01

    A set of hindcast simulations with the new version 3.6 of the Nucleus for European Modelling of the Ocean (NEMO) ocean-ice model in the ORCA1 configuration and forced by the DRAKKAR Forcing Set version 5.2 (DFS5.2) atmospheric data was performed from 1958 to 2012. Simulations differed in their sea-ice component: the old standard version Louvain-la-Neuve Sea Ice Model (LIM2) and its successor LIM3. Main differences between these sea-ice models are the parameterisations of sub-grid-scale sea-ice thickness distribution, ice deformation, thermodynamic processes, and sea-ice salinity. Our main objective was to analyse the response of the ocean-ice system sensitivity to the change in sea-ice physics. Additional sensitivity simulations were carried out for the attribution of observed differences between the two main simulations.In the Arctic, NEMO-LIM3 compares better with observations by realistically reproducing the sea-ice extent decline during the last few decades due to its multi-category sea-ice thickness. In the Antarctic, NEMO-LIM3 more realistically simulates the seasonal evolution of sea-ice extent than NEMO-LIM2. In terms of oceanic properties, improvements are not as evident, although NEMO-LIM3 reproduces a more realistic hydrography in the Labrador Sea and in the Arctic Ocean, including a reduced cold temperature bias of the Arctic Intermediate Water at 250 m. In the extra-polar regions, the oceanographic conditions of the two NEMO-LIM versions remain relatively similar, although they slowly drift apart over decades. This drift is probably due to a stronger deep water formation around Antarctica in LIM3.

  17. Arhitektuurimuuseum vaatab Aasiasse / Kim Sung Hong ; intervjueerinud Karin Hallas-Murula

    Index Scriptorium Estoniae

    Kim Sung Hong

    2009-01-01

    Näituse kuraatori Kim Sung Hongiga 21. aprillini Eesti Arhitektuurimuuseumis avatud Lõuna-Korea kaasaegse arhitektuuri näitusest "Megacity network" ("Megalinna võrgustik"), Korea arhitektuurist, arhitektidest, muinsuskaitsest ja avalikust ruumist. Näituse kujundas Hwang Doo Jin

  18. The effect of sung speech on socio-communicative responsiveness in children with Autism Spectrum Disorders

    Directory of Open Access Journals (Sweden)

    Arkoprovo ePaul

    2015-10-01

    Full Text Available There is emerging evidence to demonstrate the efficacy of music based interventions for improving social functioning in children with Autism Spectrum Disorders (ASD. While this evidence lends some support in favour of using song over spoken directives in facilitating engagement and receptive intervention in ASD, there has been little research that has investigated the efficacy of such stimuli on socio-communicative responsiveness measures. Here, we present preliminary results from a pilot study which tested whether sung instruction, as compared to spoken directives, could elicit greater number of socio-communicative behaviours in young children with ASD. Using an adapted single-subject design, three children between the ages of 3 and 4 years, participated in a programme consisting of 18 sessions, of which 9 were delivered with spoken directives and 9 with sung. Sessions were counterbalanced and randomized for three play activities - block matching, picture matching and clay play. All sessions were video-recorded for post-hoc observational coding of three behavioural metrics which included performance, frequency of social gesture and eye contact. Analysis of the videos by two independent raters indicated increased socio-communicative responsiveness in terms of frequency of social gesture as well as eye contact during sung compared to spoken conditions across all participants. Our findings suggest that sung directives may play a useful role in engaging children with ASD and also serve as an effective interventional medium to enhance socio-communicative responsiveness.

  19. The Musical Foregrounding Hypothesis : How Music Influences the Perception of Sung Language

    NARCIS (Netherlands)

    Schotanus, Yke

    2015-01-01

    Abstract The question how music influences the perception of sung language is rather complicated. There are indications that music enhances lyric perception, but also that it obstructs it. Sometimes we are deeply moved by lyrics, sometimes we don’t even hear them. The linguistic concept of foregroun

  20. Children's Reading Comprehension and Narrative Recall in Sung and Spoken Story Contexts

    Science.gov (United States)

    Kouri, Theresa; Telander, Karen

    2008-01-01

    A growing number of reading professionals have advocated teaching literacy through music and song; however, little research exists supporting such practices. The purpose of this study was to determine if sung story book readings would enhance story comprehension and narrative re-tellings in children with histories of speech and language delay.…

  1. Words and melody are intertwined in perception of sung words: EEG and behavioral evidence.

    Directory of Open Access Journals (Sweden)

    Reyna L Gordon

    Full Text Available Language and music, two of the most unique human cognitive abilities, are combined in song, rendering it an ecological model for comparing speech and music cognition. The present study was designed to determine whether words and melodies in song are processed interactively or independently, and to examine the influence of attention on the processing of words and melodies in song. Event-Related brain Potentials (ERPs and behavioral data were recorded while non-musicians listened to pairs of sung words (prime and target presented in four experimental conditions: same word, same melody; same word, different melody; different word, same melody; different word, different melody. Participants were asked to attend to either the words or the melody, and to perform a same/different task. In both attentional tasks, different word targets elicited an N400 component, as predicted based on previous results. Most interestingly, different melodies (sung with the same word elicited an N400 component followed by a late positive component. Finally, ERP and behavioral data converged in showing interactions between the linguistic and melodic dimensions of sung words. The finding that the N400 effect, a well-established marker of semantic processing, was modulated by musical melody in song suggests that variations in musical features affect word processing in sung language. Implications of the interactions between words and melody are discussed in light of evidence for shared neural processing resources between the phonological/semantic aspects of language and the melodic/harmonic aspects of music.

  2. Words and melody are intertwined in perception of sung words: EEG and behavioral evidence.

    Science.gov (United States)

    Gordon, Reyna L; Schön, Daniele; Magne, Cyrille; Astésano, Corine; Besson, Mireille

    2010-03-31

    Language and music, two of the most unique human cognitive abilities, are combined in song, rendering it an ecological model for comparing speech and music cognition. The present study was designed to determine whether words and melodies in song are processed interactively or independently, and to examine the influence of attention on the processing of words and melodies in song. Event-Related brain Potentials (ERPs) and behavioral data were recorded while non-musicians listened to pairs of sung words (prime and target) presented in four experimental conditions: same word, same melody; same word, different melody; different word, same melody; different word, different melody. Participants were asked to attend to either the words or the melody, and to perform a same/different task. In both attentional tasks, different word targets elicited an N400 component, as predicted based on previous results. Most interestingly, different melodies (sung with the same word) elicited an N400 component followed by a late positive component. Finally, ERP and behavioral data converged in showing interactions between the linguistic and melodic dimensions of sung words. The finding that the N400 effect, a well-established marker of semantic processing, was modulated by musical melody in song suggests that variations in musical features affect word processing in sung language. Implications of the interactions between words and melody are discussed in light of evidence for shared neural processing resources between the phonological/semantic aspects of language and the melodic/harmonic aspects of music.

  3. Arhitektuurimuuseum vaatab Aasiasse / Kim Sung Hong ; intervjueerinud Karin Hallas-Murula

    Index Scriptorium Estoniae

    Kim Sung Hong

    2009-01-01

    Näituse kuraatori Kim Sung Hongiga 21. aprillini Eesti Arhitektuurimuuseumis avatud Lõuna-Korea kaasaegse arhitektuuri näitusest "Megacity network" ("Megalinna võrgustik"), Korea arhitektuurist, arhitektidest, muinsuskaitsest ja avalikust ruumist. Näituse kujundas Hwang Doo Jin

  4. The Musical Foregrounding Hypothesis : How Music Influences the Perception of Sung Language

    NARCIS (Netherlands)

    Schotanus, Yke

    2015-01-01

    Abstract The question how music influences the perception of sung language is rather complicated. There are indications that music enhances lyric perception, but also that it obstructs it. Sometimes we are deeply moved by lyrics, sometimes we don’t even hear them. The linguistic concept of

  5. The Musical Foregrounding Hypothesis : How Music Influences the Perception of Sung Language

    NARCIS (Netherlands)

    Schotanus, Yke

    2015-01-01

    Abstract The question how music influences the perception of sung language is rather complicated. There are indications that music enhances lyric perception, but also that it obstructs it. Sometimes we are deeply moved by lyrics, sometimes we don’t even hear them. The linguistic concept of foregroun

  6. 40Ar-39Ar age of carbonatite-alkaline magmatism in Sung Valley, Maghalaya, India

    Indian Academy of Sciences (India)

    Jyotiranjan S Ray; Kanchan Pande

    2001-09-01

    40Ar-39Ar analyses of one alkali pyroxenite whole rock and two phlogopite separates of calcite carbonatites from the Sung Valley carbonatite-alkaline complex, which is believed to be a part of the Rajmahal-Bengal-Sylhet (RBS) ood basalt province, yielded indistinguishable plateau ages of 108.8 ± 2.0 Ma, 106:4 ± 1.3 Ma and 107.5 ± 1.4 Ma, respectively. The weighted mean of these ages, 107.2 ± 0.8 Ma, is the time of emplacement of this complex. This implies that Sung Valley complex and probably other such complexes in the Assam-Meghalaya Plateau postdate the main ood basalt event (i.e., the eruption of tholeiites) in the RBS province by ∼10 Ma.

  7. [Cerebral paragonimiasis and Bo Sung Sim's hemispherectomy in Korea in 1950s-1960s].

    Science.gov (United States)

    Park, Jiyoung; Miyagawa, Takuya; Hong, Jeonghwa; Kim, Ockjoo

    2011-06-30

    This paper deals with cerebral paragonimiasis and cerebral hemispherectomy conducted as a treatment of cerebral paragonimiasis by Bo Sung Sim in Korea in 1950s-1960s. He demonstrated that cerebral hemispherectomy could be used for unilateral diffuse cerebral paragonimiasis. Sim learned cerebral hemispherectomy from Dr. L. A. French. at the University of Minnesota from 1955 to 1957 in America. The authors argues that Bo Sung Sim's introduction of cerebral hemispherectomy to Korea was not a simple application of an advanced medical technology, but a complicated and active process in that Sim used the technique to intervene intractable complications from cerebral paragonimiasis such as generalized convulsions, spastic hemiplegia and mental deterioration. Bo Sung Sim, one of the neurosurgeons of the first generation in Korea, was trained in neurology, neuropathology, neuroradiology and animal experiments as well as in neurosurgery at the University of Minnesota. After returning to Korea, Sim faced parasitic diseases, one of the most serious public health problems at that time, which were far different from what he learned in America. As a neurosurgeon, Sim tackled with parasitic diseases of the central nervous system with various diagnostics and therapeutics. In 1950s, more than one million populations suffered from pulmonary paragonimiasis acquired by eating raw crabs or by feeding juice of crushed crayfish for the treatment of measles in Korea. About 26.6 percent of people with paragonimiasis had cerebral paragonimiasis. Before bithionol therapy was introduced in 1962, neurosurgery was the only available treatment to control increased intracranial pressures, intractable epilepsy, paralysis and mental deterioration. Between 1958 to 1962, Bo Sung Sim operated on 24 patients of cerebral paragonimiasis. In two of them, he performed cerebral hemispherectomy to control intractable convulsions when he found diffuse cerebral paragonimiasis and cerebral atrophy at the

  8. 77 FR 6782 - In the Matter of: Kok Tong Lim, a/k/a Thomas Lim Blk 258A Compassvale Road #07-551 Singapore...

    Science.gov (United States)

    2012-02-09

    ... Road 07-551 Singapore 541258; Order Denying Export Privileges On October 9, 2009, in the U.S. District Court, District of Minnesota, Kok Tong Lim, a/k/a Thomas Lim, (``Lim'') of Singapore, pled guilty to one... China Academy of Space Technology. Lim was sentenced for a prison term of time served, which amounted...

  9. La LIM in classe: un catalogo possibile

    Directory of Open Access Journals (Sweden)

    Teresa Magnaterra

    2013-03-01

    Full Text Available L’articolo propone un bilancio provvisorio dell’esperienza dell’autrice come tutor in corsi di formazione all’uso della LIM per docenti di scuola primaria e secondaria di I grado. La metodologia scelta è quella di costruire classificazioni sulla base di criteri e punti di vista differenti, che sono emersi durante i percorsi di formazione e sono stati discussi e condivisi con i docenti. Lo scopo è quello di individuare una serie di pratiche didattiche da attivare in classe in modo mirato e consapevole. L’impatto che la lavagna interattiva multimediale ha avuto fra gli insegnanti ha prodotto reazioni di entusiasmo e anche di rinuncia; ha coltivato però, fra gli stessi insegnanti, l’esigenza, e in alcuni casi la necessità, di rivedere non solo il rapporto con gli strumenti da adottare, ma anche con l’intera impostazione didattica. La sfida dunque è duplice: da una parte gli insegnanti cercano di imparare a usare la LIM, dall’altra essi, anche attraverso la lavagna interattiva, possono esplorare percorsi didattici che promuovano un apprendimento critico e creativo.

  10. Diverse expression patterns of LIM-homeodomain transcription factors (LIM-HDs) in mammalian inner ear development.

    Science.gov (United States)

    Huang, Mingqian; Sage, Cyrille; Li, Huawei; Xiang, Mengquig; Heller, Stefan; Chen, Zheng-Yi

    2008-11-01

    LIM-homeodomain transcription factors (LIM-HDs) are essential in tissue patterning and differentiation. But their expression patterns in the inner ear are largely unknown. Here we report on a study of twelve LIM-HDs, by their tempo-spatial patterns that imply distinct yet overlapping roles, in the developing mouse inner ear. Expression of Lmx1a and Isl1 begins in the otocyst stage, with Lmx1a exclusively in the non-sensory and Isl1 in the prosensory epithelia. The second wave of expression at E12.5 includes Lhx3, 5, 9, Isl2, and Lmx1b in the differentiating sensory epithelia with cellular specificities. With the exception of Lmx1a and Lhx3, all LIM-HDs are expressed in ganglion neurons. Expression of multiple LIM-HDs within a cell type suggests their redundant function.

  11. Laboratory Information Management System (LIMS): A case study

    Science.gov (United States)

    Crandall, Karen S.; Auping, Judith V.; Megargle, Robert G.

    1987-01-01

    In the late 70's, a refurbishment of the analytical laboratories serving the Materials Division at NASA Lewis Research Center was undertaken. As part of the modernization efforts, a Laboratory Information Management System (LIMS) was to be included. Preliminary studies indicated a custom-designed system as the best choice in order to satisfy all of the requirements. A scaled down version of the original design has been in operation since 1984. The LIMS, a combination of computer hardware, provides the chemical characterization laboratory with an information data base, a report generator, a user interface, and networking capabilities. This paper is an account of the processes involved in designing and implementing that LIMS.

  12. Acoustic Features and Perceptive Cues of Songs and Dialogues in Whistled Speech: Convergences with Sung Speech

    CERN Document Server

    Meyer, Julien

    2007-01-01

    Whistled speech is a little studied local use of language shaped by several cultures of the world either for distant dialogues or for rendering traditional songs. This practice consists of an emulation of the voice thanks to a simple modulated pitch. It is therefore the result of a transformation of the vocal signal that implies simplifications in the frequency domain. The whistlers adapt their productions to the way each language combines the qualities of height perceived simultaneously by the human ear in the complex frequency spectrum of the spoken or sung voice (pitch, timbre). As a consequence, this practice underlines key acoustic cues for the intelligibility of the concerned languages. The present study provides an analysis of the acoustic and phonetic features selected by whistled speech in several traditions either in purely oral whistles (Spanish, Turkish, Mazatec) or in whistles produced with an instrument like a leaf (Akha, Hmong). It underlines the convergences with the strategies of the singing ...

  13. Measurements of vibrato parameters in long sustained crescendo notes as sung by ten sopranos.

    Science.gov (United States)

    Bretos, Jose; Sundberg, Johan

    2003-09-01

    Two high-pitched and long sustained notes, F5 and A5, were selected from an aria found in ten commercial recordings of G Verdi's opera Aida by ten different sopranos. Both notes were sung without any instrumental accompaniment and with a crescendo. These audio examples were analyzed with regard to fundamental frequency, vibrato rate, vibrato extent, intonation and sound level, and the relationship among these parameters. The results reveal that vibrato rate differed significantly between the tones in most of the singers and confirm Prame's observations that vibrato rate tends to increase exponentially toward the end of tones. Moreover, both vibrato extent and mean F0 often varied systematically with sound level. The regularity of the vibrato tended to be greater at F5 than at A5.

  14. Implementing the LIM code: the structural basis for cell type-specific assembly of LIM-homeodomain complexes

    Energy Technology Data Exchange (ETDEWEB)

    Bhati, Mugdha; Lee, Christopher; Nancarrow, Amy L.; Lee, Mihwa; Craig, Vanessa J.; Bach, Ingolf; Guss, J. Mitchell; Mackay, Joel P.; Matthews, Jacqueline M. (UMASS, MED); (Sydney)

    2008-09-03

    LIM-homeodomain (LIM-HD) transcription factors form a combinatorial 'LIM code' that contributes to the specification of cell types. In the ventral spinal cord, the binary LIM homeobox protein 3 (Lhx3)/LIM domain-binding protein 1 (Ldb1) complex specifies the formation of V2 interneurons. The additional expression of islet-1 (Isl1) in adjacent cells instead specifies the formation of motor neurons through assembly of a ternary complex in which Isl1 contacts both Lhx3 and Ldb1, displacing Lhx3 as the binding partner of Ldb1. However, little is known about how this molecular switch occurs. Here, we have identified the 30-residue Lhx3-binding domain on Isl1 (Isl1{sub LBD}). Although the LIM interaction domain of Ldb1 (Ldb1{sub LID}) and Isl1{sub LBD} share low levels of sequence homology, X-ray and NMR structures reveal that they bind Lhx3 in an identical manner, that is, Isl1{sub LBD} mimics Ldb1{sub LID}. These data provide a structural basis for the formation of cell type-specific protein-protein interactions in which unstructured linear motifs with diverse sequences compete to bind protein partners. The resulting alternate protein complexes can target different genes to regulate key biological events.

  15. The LIM Homeodomain Transcription Factor LHX6

    Science.gov (United States)

    Zhang, Zichao; Gutierrez, Diana; Li, Xiao; Bidlack, Felicitas; Cao, Huojun; Wang, Jianbo; Andrade, Kelsey; Margolis, Henry C.; Amendt, Brad A.

    2013-01-01

    LHX6 is a LIM-homeobox transcription factor expressed during embryogenesis; however, the molecular mechanisms regulating LHX6 transcriptional activities are unknown. LHX6 and the PITX2 homeodomain transcription factor have overlapping expression patterns during tooth and craniofacial development, and in this report, we demonstrate new transcriptional mechanisms for these factors. PITX2 and LHX6 are co-expressed in the oral and dental epithelium and epithelial cell lines. Lhx6 expression is increased in Pitx2c transgenic mice and decreased in Pitx2 null mice. PITX2 activates endogenous Lhx6 expression and the Lhx6 promoter, whereas LHX6 represses its promoter activity. Chromatin immunoprecipitation experiments reveal endogenous PITX2 binding to the Lhx6 promoter. LHX6 directly interacts with PITX2 to inhibit PITX2 transcriptional activities and activation of multiple promoters. Bimolecular fluorescence complementation assays reveal an LHX6·PITX2 nuclear interaction in living cells. LHX6 has a dominant repressive effect on the PITX2 synergistic activation with LEF-1 and β-catenin co-factors. Thus, LHX6 acts as a transcriptional repressor and represses the expression of several genes involved in odontogenesis. We have identified specific defects in incisor, molar, mandible, bone, and root development and late stage enamel formation in Lhx6 null mice. Amelogenin and ameloblastin expression is reduced and/or delayed in the Lhx6 null mice, potentially resulting from defects in dentin deposition and ameloblast differentiation. Our results demonstrate that LHX6 regulates cell proliferation in the cervical loop and promotes cell differentiation in the anterior region of the incisor. We demonstrate new molecular mechanisms for LHX6 and an interaction with PITX2 for normal craniofacial and tooth development. PMID:23229549

  16. The word and music of epic song: From syncretism to sung poetry

    Directory of Open Access Journals (Sweden)

    Lajić-Mihajlović Danka

    2013-01-01

    Full Text Available This study concerns the relationship between verbal and musical components of Serbian epic songs with rhythm seen as a morphological dominant. My aim is to enrich the musicological inquiry of this issue and provide a contribution that complements existing folkloristic and philological research outcomes. In terms of methodology, the study promotes the necessity of recording the performers’ recited versions of songs for the purposes of investigating the relationships between verbal and musical communication, as well as an interdisciplinary approach to these issues. Two paradigmatic examples are examined, each performed by the guslar representative of his respective period of guslar practice: Tanasije Vućić (1883-1937 and Boško Vujačić (b. 1947. The observed periods span less than one century, yielding an insignificant passage of time in terms of epic historicism, but nonetheless indicate significant differences between these two guslars’ sung and narrated rhythms. Compared to Vujačić, Vućić’s singing demonstrates a considerably smaller range of the absolute duration of the sung syllables (and therefore of an entire verse, and subsequently a much stricter syllabicity. Furthermore, Vućić’s singing reflects the ideal type of “isochronous pulse in duple meter” in a rather high degree, while Vujačić more consistently refers to narration, whereas the trochaic tendency is noticeable only at the initial part of the verse. The connections between these focused individual styles are discussed through the lens of guslars’ transition from amateur to professional capacity and the changed function of epics. Considering the consequences of the policy of invalid representation and experiencing epic song as poetry (void of musical component, and on the other hand, the effects of the strategy insistent on “citatory” (verbatim treatment of poetic templates that leads to suppressing poetic creativity, I intend to draw attention of the

  17. Charakterisierung eines ultrahochmolekularen Polymethacrylats mit flüssigkristalliner Seitengruppe in verdünnter Lösung

    OpenAIRE

    Wenzel, Ulrike

    2000-01-01

    Durch radikalische Schmelzpolymerisation und anschließende Fällungsfraktionierung gewonnene ultrahochmolekulare Fraktionen des seitengruppenflüssigkristallinen Polymers Polymethacryloyloxy-hexadiyloxy-4´-methoxyphenylbenzoat (PMA-C6) wurden in verdünnter Lösung mittels Gelpermeationschromatographie, statischer Lichtstreuung und viskosimetrischen Methoden untersucht. Die thermodynamische Güte der untersuchten Lösemittel für PMA-C6 nimmt mit der Polarität in der Reihenfolge Toluol < Benzol < TH...

  18. Magic Realism, Neurodiversity, and Carnivalesque in James McBride’s Song Yet Sung

    Directory of Open Access Journals (Sweden)

    Yuan-Chin Chang

    2016-04-01

    Full Text Available This paper considers James McBride’s novel Song Yet Sung through multiple lenses – Bakhtin’s Carnivalesque, magic realism and performativity as it relates to race and gender identities. It is considered how the character of the “Dreamer” can be read clinically as suffering the sequelae of a traumatic brain injury. Her symptoms, which include future hallucinations or prophecies, can be read as neurological symptoms of her multiple head injuries documented in the novel. Connected to this reading, the influence of magic realism is considered, particularly as it relates to the natural imagery and symbolism in the novel. The importance of birds, in particular, is considered. Carnivalesque as conceptualized by Bakhtin is also considered in the context of “magical” thinking and reading, and its connections to social subversion; this is considered in relation to the era of slavery, its legacy, and associated issues of gender and race.Keywords: Carnivalesque, Magic Realism, Prophecy, Gender Performance, Racial Performativity, Identity Performance

  19. LimsPortal and BonsaiLIMS: development of a lab information management system for translational medicine

    Directory of Open Access Journals (Sweden)

    Afzal Vackar

    2011-05-01

    Full Text Available Abstract Background Laboratory Information Management Systems (LIMS are an increasingly important part of modern laboratory infrastructure. As typically very sophisticated software products, LIMS often require considerable resources to select, deploy and maintain. Larger organisations may have access to specialist IT support to assist with requirements elicitation and software customisation, however smaller groups will often have limited IT support to perform the kind of iterative development that can resolve the difficulties that biologists often have when specifying requirements. Translational medicine aims to accelerate the process of treatment discovery by bringing together multiple disciplines to discover new approaches to treating disease, or novel applications of existing treatments. The diverse set of disciplines and complexity of processing procedures involved, especially with the use of high throughput technologies, bring difficulties in customizing a generic LIMS to provide a single system for managing sample related data within a translational medicine research setting, especially where limited IT support is available. Results We have designed and developed a LIMS, BonsaiLIMS, around a very simple data model that can be easily implemented using a variety of technologies, and can be easily extended as specific requirements dictate. A reference implementation using Oracle 11 g database and the Python framework, Django is presented. Conclusions By focusing on a minimal feature set and a modular design we have been able to deploy the BonsaiLIMS system very quickly. The benefits to our institute have been the avoidance of the prolonged implementation timescales, budget overruns, scope creep, off-specifications and user fatigue issues that typify many enterprise software implementations. The transition away from using local, uncontrolled records in spreadsheet and paper formats to a centrally held, secured and backed-up database brings the

  20. LimsPortal and BonsaiLIMS: development of a lab information management system for translational medicine.

    Science.gov (United States)

    Bath, Timothy G; Bozdag, Selcuk; Afzal, Vackar; Crowther, Daniel

    2011-05-13

    Laboratory Information Management Systems (LIMS) are an increasingly important part of modern laboratory infrastructure. As typically very sophisticated software products, LIMS often require considerable resources to select, deploy and maintain. Larger organisations may have access to specialist IT support to assist with requirements elicitation and software customisation, however smaller groups will often have limited IT support to perform the kind of iterative development that can resolve the difficulties that biologists often have when specifying requirements. Translational medicine aims to accelerate the process of treatment discovery by bringing together multiple disciplines to discover new approaches to treating disease, or novel applications of existing treatments. The diverse set of disciplines and complexity of processing procedures involved, especially with the use of high throughput technologies, bring difficulties in customizing a generic LIMS to provide a single system for managing sample related data within a translational medicine research setting, especially where limited IT support is available. We have designed and developed a LIMS, BonsaiLIMS, around a very simple data model that can be easily implemented using a variety of technologies, and can be easily extended as specific requirements dictate. A reference implementation using Oracle 11 g database and the Python framework, Django is presented. By focusing on a minimal feature set and a modular design we have been able to deploy the BonsaiLIMS system very quickly. The benefits to our institute have been the avoidance of the prolonged implementation timescales, budget overruns, scope creep, off-specifications and user fatigue issues that typify many enterprise software implementations. The transition away from using local, uncontrolled records in spreadsheet and paper formats to a centrally held, secured and backed-up database brings the immediate benefits of improved data visibility, audit and

  1. LimsPortal and BonsaiLIMS: development of a lab information management system for translational medicine

    Science.gov (United States)

    2011-01-01

    Background Laboratory Information Management Systems (LIMS) are an increasingly important part of modern laboratory infrastructure. As typically very sophisticated software products, LIMS often require considerable resources to select, deploy and maintain. Larger organisations may have access to specialist IT support to assist with requirements elicitation and software customisation, however smaller groups will often have limited IT support to perform the kind of iterative development that can resolve the difficulties that biologists often have when specifying requirements. Translational medicine aims to accelerate the process of treatment discovery by bringing together multiple disciplines to discover new approaches to treating disease, or novel applications of existing treatments. The diverse set of disciplines and complexity of processing procedures involved, especially with the use of high throughput technologies, bring difficulties in customizing a generic LIMS to provide a single system for managing sample related data within a translational medicine research setting, especially where limited IT support is available. Results We have designed and developed a LIMS, BonsaiLIMS, around a very simple data model that can be easily implemented using a variety of technologies, and can be easily extended as specific requirements dictate. A reference implementation using Oracle 11 g database and the Python framework, Django is presented. Conclusions By focusing on a minimal feature set and a modular design we have been able to deploy the BonsaiLIMS system very quickly. The benefits to our institute have been the avoidance of the prolonged implementation timescales, budget overruns, scope creep, off-specifications and user fatigue issues that typify many enterprise software implementations. The transition away from using local, uncontrolled records in spreadsheet and paper formats to a centrally held, secured and backed-up database brings the immediate benefits of

  2. Cognitive priming in sung and instrumental music: activation of inferior frontal cortex.

    Science.gov (United States)

    Tillmann, B; Koelsch, S; Escoffier, N; Bigand, E; Lalitte, P; Friederici, A D; von Cramon, D Y

    2006-07-15

    Neural correlates of the processing of musical syntax-like structures have been investigated via expectancy violation due to musically unrelated (i.e., unexpected) events in musical contexts. Previous studies reported the implication of inferior frontal cortex in musical structure processing. However - due to the strong musical manipulations - activations might be explained by sensory deviance detection or repetition priming. Our present study investigated neural correlates of musical structure processing with subtle musical violations in a musical priming paradigm. Instrumental and sung sequences ended on related and less-related musical targets. The material controlled sensory priming components, and differences in target processing required listeners' knowledge on musical structures. Participants were scanned with functional Magnetic Resonance Imaging (fMRI) while performing speeded phoneme and timbre identification judgments on the targets. Behavioral results acquired in the scanner replicated the facilitation effect of related over less-related targets. The blood oxygen level-dependent (BOLD) signal linked to target processing revealed activation of right inferior frontal areas (i.e., inferior frontal gyrus, frontal operculum, anterior insula) that was stronger for less-related than for related targets, and this was independent of the material carrying the musical structures. This outcome points to the implication of inferior frontal cortex in the processing of syntactic relations also for musical material and to its role in the processing and integration of sequential information over time. In addition to inferior frontal activation, increased activation was observed in orbital gyrus, temporal areas (anterior superior temporal gyrus, posterior superior temporal gyrus and sulcus, posterior middle temporal gyrus) and supramarginal gyrus.

  3. Uncovering phenotypes of poor-pitch singing: The Sung Performance Battery (SPB

    Directory of Open Access Journals (Sweden)

    Magdalena eBerkowska

    2013-10-01

    Full Text Available Singing is as natural as speaking for humans. Increasing evidence shows that the layman can carry a tune (e.g., when asked to sing a well-known song or to imitate single pitches, intervals and short melodies. Yet, important individual differences exist in the general population with regard to singing proficiency. Some individuals are particularly inaccurate or imprecise in producing or imitating pitch information (poor-pitch singers, thus showing a variety of singing phenotypes. Unfortunately, so far there is not a standard set of tasks for assessing singing proficiency in the general population, allowing to uncover and characterize individual profiles of poor-pitch singing. Different tasks and analysis methods are typically used in various experiments, making the comparison of the results across studies arduous. To fill this gap we propose here a new tool for assessing singing proficiency (the Sung Performance Battery, SPB. The SPB starts from the assessment of participants’ vocal range followed by five tasks: 1 single-pitch matching, 2 pitch-interval matching, 3 novel-melody matching, 4 singing from memory of familiar melodies (with lyrics and on a syllable, and 5 singing of familiar melodies (with lyrics and on a syllable at a slow tempo indicated by a metronome. Data analysis via acoustical methods provides objective measures of pitch accuracy and precision in terms of absolute and relative pitch. The SPB has been tested in a group of 50 occasional singers. The results indicate that the battery is useful for characterizing proficient singing and for detecting cases of inaccurate and/or imprecise singing.

  4. Uncovering phenotypes of poor-pitch singing: the Sung Performance Battery (SPB)

    Science.gov (United States)

    Berkowska, Magdalena; Dalla Bella, Simone

    2013-01-01

    Singing is as natural as speaking for humans. Increasing evidence shows that the layman can carry a tune (e.g., when asked to sing a well-known song or to imitate single pitches, intervals and short melodies). Yet, important individual differences exist in the general population with regard to singing proficiency. Some individuals are particularly inaccurate or imprecise in producing or imitating pitch information (poor-pitch singers), thus showing a variety of singing phenotypes. Unfortunately, so far there is not a standard set of tasks for assessing singing proficiency in the general population, allowing to uncover and characterize individual profiles of poor-pitch singing. Different tasks and analysis methods are typically used in various experiments, making the comparison of the results across studies arduous. To fill this gap we propose here a new tool for assessing singing proficiency (the Sung Performance Battery, SPB). The SPB starts from the assessment of participants' vocal range followed by five tasks: (1) single-pitch matching, (2) pitch-interval matching, (3) novel-melody matching, (4) singing from memory of familiar melodies (with lyrics and on a syllable), and (5) singing of familiar melodies (with lyrics and on a syllable) at a slow tempo indicated by a metronome. Data analysis via acoustical methods provides objective measures of pitch accuracy and precision in terms of absolute and relative pitch. The SPB has been tested in a group of 50 occasional singers. The results indicate that the battery is useful for characterizing proficient singing and for detecting cases of inaccurate and/or imprecise singing. PMID:24151475

  5. The Evolutionarily Conserved LIM Homeodomain Protein LIM-4/LHX6 Specifies the Terminal Identity of a Cholinergic and Peptidergic C. elegans Sensory/Inter/Motor Neuron-Type

    Science.gov (United States)

    Choi, Seong-Kyoon; Huh, Yang Hoon; Fang, Zi; Park, Seo Jin; Kim, Myoung Ok; Ryoo, Zae Young; Kang, Kyeongjin; Kweon, Hee-Seok; Jeon, Won Bae; Li, Chris; Kim, Kyuhyung

    2015-01-01

    The expression of specific transcription factors determines the differentiated features of postmitotic neurons. However, the mechanism by which specific molecules determine neuronal cell fate and the extent to which the functions of transcription factors are conserved in evolution are not fully understood. In C. elegans, the cholinergic and peptidergic SMB sensory/inter/motor neurons innervate muscle quadrants in the head and control the amplitude of sinusoidal movement. Here we show that the LIM homeobox protein LIM-4 determines neuronal characteristics of the SMB neurons. In lim-4 mutant animals, expression of terminal differentiation genes, such as the cholinergic gene battery and the flp-12 neuropeptide gene, is completely abolished and thus the function of the SMB neurons is compromised. LIM-4 activity promotes SMB identity by directly regulating the expression of the SMB marker genes via a distinct cis-regulatory motif. Two human LIM-4 orthologs, LHX6 and LHX8, functionally substitute for LIM-4 in C. elegans. Furthermore, C. elegans LIM-4 or human LHX6 can induce cholinergic and peptidergic characteristics in the human neuronal cell lines. Our results indicate that the evolutionarily conserved LIM-4/LHX6 homeodomain proteins function in generation of precise neuronal subtypes. PMID:26305787

  6. Der Tod im Leben. Einige Grundgedanken zu und aus Franz Rosenzweigs der Stern der Erlösung

    Directory of Open Access Journals (Sweden)

    Brasser Martin

    2012-01-01

    Full Text Available Franz Rosenzweig gilt als Existenzphilosoph, weil er das Thema des Todes ins Zentrum der Argumentation in seinem philosophisch-theologischen Hauptwerk Der Stern der Erlösung gestellt hat. Die Religion wird dort als der Ort verstanden, an dem der Tod nicht mehr wie im a-religiösen Leben aus dem Leben herausgedrängt werden muss. Wenn Religion Liebe ist, dann ist der Tod so Teil des Lebens wie er Teil der Liebe zwischen zwei Liebenden ist: als bejahter Bestandteil ihres gemeinsamen Lebens. Der Aufsatz versucht diese Argumentation nachzuzeichnen und zu plausibilisieren.

  7. Regulation of Estrogen-Dependent Transcription by the LIM Cofactors CLIM and RLIM in Breast Cancer

    Science.gov (United States)

    Johnsen, Steven A.; Güngör, Cenap; Prenzel, Tanja; Riethdorf, Sabine; Riethdorf, Lutz; Taniguchi-Ishigaki, Naoko; Rau, Thomas; Tursun, Baris; Furlow, J. David; Sauter, Guido; Scheffner, Martin; Pantel, Klaus; Gannon, Frank; Bach, Ingolf

    2009-01-01

    Mammary oncogenesis is profoundly influenced by signaling pathways controlled by Estrogen Receptor-alpha (ERα). Although it is known that ERα exerts its oncogenic effect by stimulating the proliferation of many human breast cancers through the activation of target genes, our knowledge of the underlying transcriptional mechanisms remains limited. Our published work has shown that the in vivo activity of LIM homeodomain transcription factors (LIM-HDs) is critically regulated by Cofactors of LIM-HD proteins (CLIM) and the ubiquitin ligase RING finger LIM domain interacting protein (RLIM). Here, we identify CLIM and RLIM as novel ERα cofactors that co-localize and interact with ERα in primary human breast tumors. We show that both cofactors associate with estrogen responsive promoters and regulate the expression of endogenous ERα target genes in breast cancer cells. Surprisingly, our results indicate opposing functions of LIM cofactors for ERα and LIM-HDs: whereas CLIM enhances transcriptional activity of LIM-HDs, it inhibits transcriptional activation mediated by ERα on most target genes in vivo. In turn, the ubiquitin ligase RLIM inhibits transcriptional activity of LIM-HDs, but enhances transcriptional activation of endogenous ERα target genes. Results from a human breast cancer tissue microarray (TMA) of 1,335 patients revealed a highly significant correlation of elevated CLIM levels to ER/PR positivity and poor differentiation of tumors. Combined, these results indicate that LIM cofactors CLIM and RLIM regulate the biological activity of ERα during the development of human breast cancer. PMID:19117995

  8. Sustainable Land Management in the Lim River Basin

    Science.gov (United States)

    Grujic, Gordana; Petkovic, Sava; Tatomir, Uros

    2017-04-01

    In the cross-border belt between Serbia and Montenegro are located more than one hundred torrential water flows that belong to the Lim River Basin. Under extreme climate events they turned into floods of destructive power and great energy causing enormous damage on the environment and socio-economic development in the wider region of the Western Balkans. In addition, anthropogenic factors influence the land instability, erosion of river beds and loss of topsoil. Consequently, this whole area is affected by pluvial and fluvial erosion of various types and intensity. Terrain on the slopes over 5% is affected by intensive degree of erosion, while strong to medium degree covers 70% of the area. Moreover, in the Lim River Basin were built several hydro-energetic systems and accumulations which may to a certain extent successfully regulate the water regime downstream and to reduce the negative impact on the processes of water erosion. However, siltation of accumulation reduces their useful volume and threatens the basic functions (water reservoirs), especially those ones for water supply, irrigation and energy production that have lost a significant part of the usable volume due to accumulated sediments. Facing the negative impacts of climate change and human activities on the process of land degradation in the Lim River basin imposes urgent need of adequate preventive and protective measures at the local and regional level, which can be effectively applied only through enhanced cross-border cooperation among affected communities in the region. The following set of activities were analyzed to improve the actual management of river catchment: Identifying priorities in the spatial planning, land use and water resources management while respecting the needs of local people and the communities in the cross border region; development of cooperation and partnership between the local population, owners and users of real estate (pastures, agricultural land, forests, fisheries

  9. LIM only 4 is overexpressed in late stage pancreas cancer

    Directory of Open Access Journals (Sweden)

    Fujita Hayato

    2008-12-01

    Full Text Available Abstract Background LIM-only 4 (LMO4, a member of the LIM-only (LMO subfamily of LIM domain-containing transcription factors, was initially reported to have an oncogenic role in breast cancer. We hypothesized that LMO4 may be related to pancreatic carcinogenesis as it is in breast carcinogenesis. If so, this could result in a better understanding of tumorigenesis in pancreatic cancer. Methods We measured LMO4 mRNA levels in cultured cells, pancreatic bulk tissues and microdissected target cells (normal ductal cells; pancreatic intraepithelial neoplasia-1B [PanIN-1B] cells; PanIN-2 cells; invasive ductal carcinoma [IDC] cells; intraductal papillary-mucinous adenoma [IPMA] cells; IPM borderline [IPMB] cells; and invasive and non-invasive IPM carcinoma [IPMC] by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR. Results 9 of 14 pancreatic cancer cell lines expressed higher levels of LMO4 mRNA than did the human pancreatic ductal epithelial cell line (HPDE. In bulk tissue samples, expression of LMO4 was higher in pancreatic carcinoma than in intraductal papillary-mucinous neoplasm (IPMN or non-neoplastic pancreas (p LMO4 than did normal ductal epithelia or PanIN-1B cells (p p = 0.014. IPMC cells expressed significantly higher levels of LMO4 than did normal ductal epithelia (p p p = 0.003. Conclusion Pancreatic carcinomas (both IDC and IPMC expressed significantly higher levels of LMO4 mRNA than did normal ductal epithelia, PanIN-1B, PanIN-2, IPMA and IPMB. These results suggested that LMO4 is overexpressed at late stages in carcinogenesis of pancreatic cancer.

  10. MetaLIMS, a simple open-source laboratory information management system for small metagenomic labs.

    Science.gov (United States)

    Heinle, Cassie Elizabeth; Gaultier, Nicolas Paul Eugène; Miller, Dana; Purbojati, Rikky Wenang; Lauro, Federico M

    2017-06-01

    As the cost of sequencing continues to fall, smaller groups increasingly initiate and manage larger sequencing projects and take on the complexity of data storage for high volumes of samples. This has created a need for low-cost laboratory information management systems (LIMS) that contain flexible fields to accommodate the unique nature of individual labs. Many labs do not have a dedicated information technology position, so LIMS must also be easy to setup and maintain with minimal technical proficiency. MetaLIMS is a free and open-source web-based application available via GitHub. The focus of MetaLIMS is to store sample metadata prior to sequencing and analysis pipelines. Initially designed for environmental metagenomics labs, in addition to storing generic sample collection information and DNA/RNA processing information, the user can also add fields specific to the user's lab. MetaLIMS can also produce a basic sequencing submission form compatible with the proprietary Clarity LIMS system used by some sequencing facilities. To help ease the technical burden associated with web deployment, MetaLIMS options the use of commercial web hosting combined with MetaLIMS bash scripts for ease of setup. MetaLIMS overcomes key challenges common in LIMS by giving labs access to a low-cost and open-source tool that also has the flexibility to meet individual lab needs and an option for easy deployment. By making the web application open source and hosting it on GitHub, we hope to encourage the community to build upon MetaLIMS, making it more robust and tailored to the needs of more researchers.

  11. LIMS-实验室管理未来展望%Laboratory ManagementLIMS-Future Prospect of Laboratory Management

    Institute of Scientific and Technical Information of China (English)

    毕玉春; 萧亮

    2009-01-01

    @@ 0 引言 LIMS(Laboratory Information Management System) 即实验室信息管理系统,是通过对样品检验流程、分析数据及报告、实验室资源和客户信息等要素的综合管理,按照标准化实验室管理规范,建立符合实验室业务流程的质量体系,实现实验室信息化管理.

  12. Perception of a Sung Vowel as a Function of Frequency-Modulation Rate and Excursion in Listeners with Normal Hearing and Hearing Impairment

    Science.gov (United States)

    Vatti, Marianna; Santurette, Sébastien; Pontoppidan, Niels Henrik; Dau, Torsten

    2014-01-01

    Purpose: Frequency fluctuations in human voices can usually be described as coherent frequency modulation (FM). As listeners with hearing impairment (HI listeners) are typically less sensitive to FM than listeners with normal hearing (NH listeners), this study investigated whether hearing loss affects the perception of a sung vowel based on FM…

  13. A Way to Measure the Residual Flow in the Lim Fjord

    DEFF Research Database (Denmark)

    Larsen, Torben

    The Lim fjord in Denmark has two connections to the open sea. Therefore the residual flow through the Lim fjord is an important parameter in relation to salinity and concentration on nutrients. It is described how it has been possible to determine the residual flow from the observation...

  14. Early evolution of the LIM homeobox gene family

    Energy Technology Data Exchange (ETDEWEB)

    Srivastava, Mansi; Larroux, Claire; Lu, Daniel R; Mohanty, Kareshma; Chapman, Jarrod; Degnan, Bernard M; Rokhsar, Daniel S

    2010-01-01

    LIM homeobox (Lhx) transcription factors are unique to the animal lineage and have patterning roles during embryonic development in flies, nematodes and vertebrates, with a conserved role in specifying neuronal identity. Though genes of this family have been reported in a sponge and a cnidarian, the expression patterns and functions of the Lhx family during development in non-bilaterian phyla are not known. We identified Lhx genes in two cnidarians and a placozoan and report the expression of Lhx genes during embryonic development in Nematostella and the demosponge Amphimedon. Members of the six major LIM homeobox subfamilies are represented in the genomes of the starlet sea anemone, Nematostella vectensis, and the placozoan Trichoplax adhaerens. The hydrozoan cnidarian, Hydra magnipapillata, has retained four of the six Lhx subfamilies, but apparently lost two others. Only three subfamilies are represented in the haplosclerid demosponge Amphimedon queenslandica. A tandem cluster of three Lhx genes of different subfamilies and a gene containing two LIM domains in the genome of T. adhaerens (an animal without any neurons) indicates that Lhx subfamilies were generated by tandem duplication. This tandem cluster in Trichoplax is likely a remnant of the original chromosomal context in which Lhx subfamilies first appeared. Three of the six Trichoplax Lhx genes are expressed in animals in laboratory culture, as are all Lhx genes in Hydra. Expression patterns of Nematostella Lhx genes correlate with neural territories in larval and juvenile polyp stages. In the aneural demosponge, A. queenslandica, the three Lhx genes are expressed widely during development, including in cells that are associated with the larval photosensory ring. The Lhx family expanded and diversified early in animal evolution, with all six subfamilies already diverged prior to the cnidarian-placozoan-bilaterian last common ancestor. In Nematostella, Lhx gene expression is correlated with neural

  15. Early evolution of the LIM homeobox gene family

    Directory of Open Access Journals (Sweden)

    Degnan Bernard M

    2010-01-01

    Full Text Available Abstract Background LIM homeobox (Lhx transcription factors are unique to the animal lineage and have patterning roles during embryonic development in flies, nematodes and vertebrates, with a conserved role in specifying neuronal identity. Though genes of this family have been reported in a sponge and a cnidarian, the expression patterns and functions of the Lhx family during development in non-bilaterian phyla are not known. Results We identified Lhx genes in two cnidarians and a placozoan and report the expression of Lhx genes during embryonic development in Nematostella and the demosponge Amphimedon. Members of the six major LIM homeobox subfamilies are represented in the genomes of the starlet sea anemone, Nematostella vectensis, and the placozoan Trichoplax adhaerens. The hydrozoan cnidarian, Hydra magnipapillata, has retained four of the six Lhx subfamilies, but apparently lost two others. Only three subfamilies are represented in the haplosclerid demosponge Amphimedon queenslandica. A tandem cluster of three Lhx genes of different subfamilies and a gene containing two LIM domains in the genome of T. adhaerens (an animal without any neurons indicates that Lhx subfamilies were generated by tandem duplication. This tandem cluster in Trichoplax is likely a remnant of the original chromosomal context in which Lhx subfamilies first appeared. Three of the six Trichoplax Lhx genes are expressed in animals in laboratory culture, as are all Lhx genes in Hydra. Expression patterns of Nematostella Lhx genes correlate with neural territories in larval and juvenile polyp stages. In the aneural demosponge, A. queenslandica, the three Lhx genes are expressed widely during development, including in cells that are associated with the larval photosensory ring. Conclusions The Lhx family expanded and diversified early in animal evolution, with all six subfamilies already diverged prior to the cnidarian-placozoan-bilaterian last common ancestor. In

  16. con trastornos de personalidad limítrofe

    Directory of Open Access Journals (Sweden)

    Claudio Martínez Guzmán

    2005-01-01

    Full Text Available A partir de una experiencia de trabajo e investigación con pacientes en crisis dentro de servicios de urgencia psiquiátricos y de hospital general se proponen ideas acerca del abordaje de la patología de personalidad limítrofe en ese contexto. Se exploran algunas ideas sobre las particularidades del llamado parasuicidio del paciente límite y se describen elementos que deberían abordarse en una intervención de esta naturaleza y que constituyen variantes en la modalidad estándar de esta clase de terapia. Se discute acerca del tema de la dosis-efecto en la psicoterapia y se propone esta técnica como puerta de entrada en servicios masivos de atención psicológica

  17. Perception of a Sung Vowel as a Function of Frequency-Modulation Rate and Excursionin Normal-Hearing and Hearing-Impaired Listeners

    DEFF Research Database (Denmark)

    Vatti, Marianna; Santurette, Sébastien; Pontoppidan, Niels henrik

    2014-01-01

    affects the perception of a sung vowel based on FM cues. Method: Vibrato maps were obtained in 14 NH and 12 HI listeners with different degrees of musical experience. The FM rate and FM excursion of a synthesized vowel, to which coherent FM was applied, were adjusted until a singing voice emerged. Results......: In NH listeners, adding FM to the steady vowel components produced perception of a singing voice for FM rates between 4.1 and 7.5 Hz and FM excursions between 17 and 83 cents on average. In contrast, HI listeners showed substantially broader vibrato maps. Individual differences in map boundaries were......, overall, not correlated with audibility or frequency selectivity at the vowel fundamental frequency, with no clear effect of musical experience. Conclusion: Overall, it was shown that hearing loss affects the perception of a sung vowel based on FM-rate and FM-excursion cues, possibly due to deficits in FM...

  18. Development of Feature Set, Classification Implementation and Applications for Vowel Migration/Modification in Sung Filipino (Tagalog Texts and Perceived Intelligibility

    Directory of Open Access Journals (Sweden)

    Virginia B. Bustos

    2009-12-01

    Full Text Available With the emergence of research on real-time visual feedback to supplement vocal pedagogy, the utilization of technology in the world of music is now seen to accelerate skills learning and enhance cognitive development. The researchers of this project aim to further analyze vowel intelligibility and develop software applications intended to be used not only by professional singers but also by individuals who wish to improve their singing capability. Data in the form of sung vowels and song pieces were obtained from 46 singers. A Listening Test was then conducted on these samples to obtain the ground truth for vowel classification based on human perception. Simulation of the human auditory perception of sung Filipino vowels was performed using formant frequencies and Mel-frequency cepstral coefficients as feature vector inputs to a two-stage Discriminant Analysis classifier. The setup resulted in an over-all Training Set accuracy of 89.4% and an over-all Test Set accuracy of 90.9%. The accuracy of the classifier, measured in terms of the correspondence of vowel classifications obtained from the classifier with the results of the Listening Test, reached 92.3%. Using information obtained from the classifier, offline and online/real-time software applications were developed. The main application features include the display of the spectral envelope and spectrogram, pitch and vibrato analysis and direct feedback on the classification of the sung vowel. These features were recommended by singers who were surveyed and were incorporated in the applications to aid singers to adjust formant locations, directly determine listener’s perception of sung vowels, perform modeling effectively and carry out vowel migration.

  19. Kalium-39-NMR in-vivo am Menschen bei 7Tesla: 39K-MR-Bildgebung und Auflösung der Quadrupolaufspaltung der 39K-Resonanz

    OpenAIRE

    Rösler, Manuela Barbara

    2015-01-01

    In dieser Arbeit wurde die Machbarkeit der In-vivo-39K-MR-Bildgebung am menschlichen Oberschenkel sowie am Kopf mit einer nominellen Auflösung von 1 cm3 in einer Messzeit von 30 min bei einer Grundmagnetfeldstärke von 7T gezeigt. Zur Optimierung der Sequenzparameter wurden sowohl die globalen Relaxationszeitkonstanten bestimmt. In Untersuchungsregionen mit hohem Muskelanteil, wie im Ober- und Unterschenkel, ist die Resonanz von 39K in ein Triplett aufgespalten. Die beiden Satellitenresona...

  20. Genome-wide identification, characterization and expression profiling of LIM family genes in Solanum lycopersicum L.

    Science.gov (United States)

    Khatun, Khadiza; Robin, Arif Hasan Khan; Park, Jong-In; Ahmed, Nasar Uddin; Kim, Chang Kil; Lim, Ki-Byung; Kim, Min-Bae; Lee, Do-Jin; Nou, Ill Sup; Chung, Mi-Young

    2016-11-01

    LIM domain proteins, some of which have been shown to be actin binding proteins, are involved in various developmental activities and cellular processes in plants. To date, the molecular defense-related functions of LIM family genes have not been investigated in any solanaceous vegetable crop species. In this study, we identified 15 LIM family genes in tomato (Solanum lycopersicum L.) through genome-wide analysis and performed expression profiling in different organs of tomato, including fruits at six different developmental stages. We also performed expression profiling of selected tomato LIM genes in plants under ABA, drought, cold, NaCl and heat stress treatment. The encoded proteins of the 15 tomato LIM genes were classified into two main groups, i.e., proteins similar to cysteine-rich proteins and plant-specific DAR proteins, based on differences in functional domains and variability in their C-terminal regions. The DAR proteins contain a so far poorly characterized zinc-finger-like motif that we propose to call DAR-ZF. Six of the 15 LIM genes were expressed only in flowers, indicating that they play flower-specific roles in plants. The other nine genes were expressed in all organs and at various stages of fruit development. SlβLIM1b was expressed relatively highly at the later stage of fruit development, but three other genes, SlWLIM2a, SlDAR2 and SlDAR4, were expressed at the early stage of fruit development. Seven genes were induced by ABA, five by cold, seven by drought, eight by NaCl and seven by heat treatment respectively, indicating their possible roles in abiotic stress tolerance. Our results will be useful for functional analysis of LIM genes during fruit development in tomato plants under different abiotic stresses. Copyright © 2016. Published by Elsevier Masson SAS.

  1. openBIS ELN-LIMS: an open-source database for academic laboratories

    OpenAIRE

    Barillari, Caterina; Ottoz, Diana S. M.; Fuentes-Serna, Juan Mariano; Ramakrishnan, Chandrasekhar; Rinn, Bernd; Rudolf, Fabian

    2015-01-01

    Summary: The open-source platform openBIS (open Biology Information System) offers an Electronic Laboratory Notebook and a Laboratory Information Management System (ELN-LIMS) solution suitable for the academic life science laboratories. openBIS ELN-LIMS allows researchers to efficiently document their work, to describe materials and methods and to collect raw and analyzed data. The system comes with a user-friendly web interface where data can be added, edited, browsed and searched. Availabil...

  2. Competitividad de las agroindustrias del limón pertenecientes al clúster del limón mexicano en Colima, México

    OpenAIRE

    Magaña Sánchez, Pablo Adrián; Padilla Bernal, Luz Evelia; José G. Vargas Hernández

    2010-01-01

    El presente trabajo se refiere al estudio del nivel de competitividad de las Agroindustrias del Limón pertenecientes al clúster del limón mexicano en Colima, México en base al modelo del IMD (International Institute for Management Development) como teoría de soporte. En este estudio se diseño y utilizó un instrumento de medición de acuerdo al modelo anteriormente mencionado adaptando las variables para el contexto de nuestro estudio. Es necesario mencionar que el modelo del IMD se utilizó par...

  3. SOA在LIMS设计中的应用%Application of SOA in design of LIMS

    Institute of Scientific and Technical Information of China (English)

    刘国庆; 钱俊

    2012-01-01

    基于模块紧密耦合设计的LIMS(laboratory information management system)存在升级维护困难、成本大等缺点,利用松散耦合的SOA(service-oriented architecture)系统架构则较好地解决了这一问题.在分析SOA优点、实现方式等基础上,探讨了基于SOA架构的LIMS开发设计,采用Web服务与XML文档交互的实现方法,给出了一种新型的实验室信息管理系统架构.南方医科大学电子创新实验室LIMS的设计开发与应用表明基于SOA的LIMS开发是很有应用前景的.%The tightly coupling LIMS(laboratory information management system) is expensive and difficult to upgrade and maintain as opposed to SOA model based on relax coupling. Following the analysis of the merits and realization way of SOA, the design of LIMS based on SOA has been discussed. Adopting the realization way of alternating the Web Service and XML document, a novel LIMS has been presented. Finally the design and application of LIMS in the Electronic Innovation Laboratory in Southern Medical University have been introduced, illustrating the wide and promising application of LIMS based on SOA.

  4. The diversification of the LIM superclass at the base of the metazoa increased subcellular complexity and promoted multicellular specialization.

    Directory of Open Access Journals (Sweden)

    Bernard J Koch

    Full Text Available BACKGROUND: Throughout evolution, the LIM domain has been deployed in many different domain configurations, which has led to the formation of a large and distinct group of proteins. LIM proteins are involved in relaying stimuli received at the cell surface to the nucleus in order to regulate cell structure, motility, and division. Despite their fundamental roles in cellular processes and human disease, little is known about the evolution of the LIM superclass. RESULTS: We have identified and characterized all known LIM domain-containing proteins in six metazoans and three non-metazoans. In addition, we performed a phylogenetic analysis on all LIM domains and, in the process, have identified a number of novel non-LIM domains and motifs in each of these proteins. Based on these results, we have formalized a classification system for LIM proteins, provided reasonable timing for class and family origin events; and identified lineage-specific loss events. Our analysis is the first detailed description of the full set of LIM proteins from the non-bilaterian species examined in this study. CONCLUSION: Six of the 14 LIM classes originated in the stem lineage of the Metazoa. The expansion of the LIM superclass at the base of the Metazoa undoubtedly contributed to the increase in subcellular complexity required for the transition from a unicellular to multicellular lifestyle and, as such, was a critically important event in the history of animal multicellularity.

  5. Expression of LIM-domain binding protein (ldb) genes during zebrafish embryogenesis.

    Science.gov (United States)

    Toyama, R; Kobayashi, M; Tomita, T; Dawid, I B

    1998-02-01

    LIM homeodomain proteins are developmental regulators whose functions depend on synergism with LIM domain binding proteins (Ldb proteins). We have isolated four members of the ldb gene family from the zebrafish, Danio rerio. Ldb1, Ldb2 and Ldb3 share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively. In overlay assays, Ldb proteins bind LIM homeodomain proteins and LMO1, but not zyxin or MLP. Whole mount in situ hybridization showed that zebrafish ldb1 is expressed ubiquitously from gastrulation onward. Ldb2 is ubiquitous at gastrulation, and later is found in many but not all tissues, especially the anterior central nervous system (CNS) and vasculature. Ldb3 mRNA was expressed primarily in the anterior CNS.

  6. On the quality of the Nimbus 7 LIMS Version 6 water vapor profiles and distributions

    Directory of Open Access Journals (Sweden)

    B. T. Marshall

    2009-12-01

    Full Text Available This report describes the quality of the Nimbus 7 Limb Infrared Monitor of the Stratosphere (LIMS water vapor (H2O profiles of 1978/79 that were processed with a Version 6 (V6 algorithm and archived in 2002. The V6 profiles incorporate a better knowledge of the instrument attitude for the LIMS measurements along its orbits, leading to improvements for its temperature profiles and for the registration of its water vapor radiances with pressure. As a result, the LIMS V6 zonal-mean distributions of H2O exhibit better hemispheric symmetry than was the case from the original Version 5 (V5 dataset that was archived in 1982. Estimates of the precision and accuracy of the V6 H2O profiles are developed and provided. Individual profiles have a precision of order 5% and an estimated accuracy of about 19% at 3 hPa, 14% at 10 hPa, and 26% at 50 hPa. Profile segments within about 2 km of the tropopause are often affected by emissions from clouds that appear in the finite field-of-view of the detector for the LIMS H2O channel. Zonally-averaged distributions of the LIMS V6 H2O are compared with those from the more recent Microwave Limb Sounder (MLS satellite experiment for November, February, and May of 2004/05. The patterns and values of their respective distributions are similar in many respects. Effects of a strengthened Brewer-Dobson circulation are indicated in the MLS distributions of the recent decade versus those of LIMS from 1978/79. A tropical tape recorder signal is present in the 7-month time series of LIMS V6 H2O with lowest values in February 1979, and the estimated, annually-averaged "entry-level" H2O is 3.5 to 3.8 ppmv. It is judged that this historic LIMS water vapor dataset is of good quality for studies of the near global-scale chemistry and transport for pressure levels from 3 hPa to about 70 to 100 hPa.

  7. The evolution of a LIMS (laboratory information management system). [Chemical analyses at BNFL

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1992-04-01

    Changes in the world and United Kingdom markets for nuclear fuels during the 1990s have prompted British Nuclear Fuels (BNFL) to maximise cost effectiveness in its Chemical and Metallurgical Services department. A laboratory information management system (LIMS) was introduced in order to keep records of analytical techniques and equipment up to date by coordinating various computer systems. Wherever possible automated systems have replaced traditional, labour intensive techniques. So successful has the LIMS system been, that the team now hopes to expand into expert systems. (UK).

  8. On the Quality of the Nimbus 7 LIMS Version 6 Water Vapor Profiles and Distributions

    Science.gov (United States)

    Remsberg, E. E.; Natarajan, M.; Lingenfelser, G. S.; Thompson, R. E.; Marshall, B. T.; Gordley, L. L.

    2009-01-01

    This report describes the quality of the Nimbus 7 Limb Infrared Monitor of the Stratosphere (LIMS) water vapor (H2O) profiles of 1978/79 that were processed with a Version 6 (V6) algorithm and archived in 2002. The V6 profiles incorporate a better knowledge of the instrument attitude for the LIMS measurements along its orbits, leading to improvements for its temperature profiles and for the registration of its water vapor radiances with pressure. As a result, the LIMS V6 zonal-mean distributions of H2O exhibit better hemispheric symmetry than was the case from the original Version 5 (V5) dataset that was archived in 1982. Estimates of the precision and accuracy of the V6 H2O profiles are developed and provided. Individual profiles have a precision of order 5% and an estimated accuracy of about 19% at 3 hPa, 14% at 10 hPa, and 26% at 50 hPa. Profile segments within about 2 km of the tropopause are often affected by emissions from clouds that appear in the finite field-of-view of the detector for the LIMS H2O channel. Zonally-averaged distributions of the LIMS V6 H2O are compared with those from the more recent Microwave Limb Sounder (MLS) satellite experiment for November, February, and May of 2004/2005. The patterns and values of their respective distributions are similar in many respects. Effects of a strengthened Brewer-Dobson circulation are indicated in the MLS distributions of the recent decade versus those of LIMS from 1978/79. A tropical tape recorder signal is present in the 7-month time series of LIMS V6 H2O with lowest values in February 1979, and the estimated, annually-averaged "entry-level" H2O is 3.5 to 3.8 ppmv. It is judged that this historic LIMS water vapor dataset is of good quality for studies of the near global-scale chemistry and transport for pressure levels from 3 hPa to about 70 to 100 hPa.

  9. [Missionary Medicine of Canadian Presbytery and Korean Doctors under Japanese Occupation--focusing Sung-jin and Ham-heung].

    Science.gov (United States)

    Heo, Yun-Jung; Cho, Young-Soo

    2015-12-01

    In East Asia during the second half of the 19th century, overseas mission work by Protestant churches thrived. Missionaries built schools and hospitals and effectively used them for evangelism. In the 20th century when Social Gospel Movement was expanding, medical work has been recognized as a significant mission service in and by itself. This article reviewed the construction and characteristics of missions work conducted by Canadian Presbytery; missionary doctors and Korean doctors who worked at the mission hospitals; why the missionary medical work had to stop; and career paths taken by Korean doctors upon liberation from Japanese occupation. The Canadian Presbytery missionaries, unlike other denomination missionaries, were rather critical of Imperial Japan, but supportive towards Koreans. This could have stemmed from the reflection of their own experience of once a colony of British Empire and also their value system that promotes egalitarian, democratic and progressive theology. The Sung-jin and Ham-heung Mission Bases were a community, interacting organically as a 'Triangle of Church, School and Hospital.' The missionaries mobilized the graduates from Christian schools and organized a Young Men's Christian Association (YMCA). Some of the graduates were trained to become medical doctors or assistants and worked at mission hospitals. Missionary doctors' approaches to balancing evangelism and medical practice varied. For example, Robert Grieson went through confusion and struggled to balance conflicting roles as a pastor for evangelism and also as a physician. Kate McMillan, on the other hand, had less burden for evangelism than Grieson, and focused on medical work by taking advantage of the opportunity that, as a woman, she can easily approach Korean women. Still another case was Florence Murray who practised evangelism within the hospital setting, and successfully carried out the role as a hospital administrator, going beyond 'women's work' as McMillan did

  10. Interaction between bottom-up and top-down effects during the processing of pitch intervals in sequences of spoken and sung syllables.

    Science.gov (United States)

    Angenstein, Nicole; Scheich, Henning; Brechmann, André

    2012-07-02

    The processing of pitch intervals may be differentially influenced when musical or speech stimuli carry the pitch information. Most insights into the neural basis of pitch interval processing come from studies on music perception. However, music, in contrast to speech, contains a stable set of pitch intervals. To converge the investigation of pitch interval processing in music and speech, we used sequences of the same spoken or sung syllables. The pitch of these syllables varied either by semitone steps like in music or by smaller intervals. Participants had to differentiate the sequences according to their different sizes of pitch intervals or to the direction of the last frequency step in the sequence. The results depended strongly on the specific task demands. Whereas the interval-size task itself recruited more regions in right lateralized fronto-parietal brain network, stronger activity on semitone than on non-semitone sequences was found in the left hemisphere (mainly in frontal cortex) during this task. These effects were also influenced by the speech mode (spoken or sung syllables). Our findings suggest that the processing of pitch intervals in sequences of syllables depends on an interaction between bottom-up (speech mode, pitch interval) and top-down effects (task). Copyright © 2012 Elsevier Inc. All rights reserved.

  11. LIM Kinase Inhibitor Pyr1 Reduces the Growth and Metastatic Load of Breast Cancers

    NARCIS (Netherlands)

    Prunier, Chloé; Josserand, Véronique; Vollaire, Julien; Beerling, Evelyne; Petropoulos, Christos; Destaing, Olivier; Montemagno, Christopher; Hurbin, Amandine; Prudent, Renaud; de Koning, Leanne; Kapur, Reuben; Cohen, Pascale A; Albiges-Rizo, Corinne; Coll, Jean-Luc; van Rheenen, Jacco; Billaud, Marc; Lafanechère, Laurence

    2016-01-01

    LIM kinases (LIMK) are emerging targets for cancer therapy, and they function as network hubs to coordinate actin and microtubule dynamics. When LIMKs are inhibited, actin microfilaments are disorganized and microtubules are stabilized. Owing to their stabilizing effect on microtubules, LIMK

  12. Mutation in LIM2 Is Responsible for Autosomal Recessive Congenital Cataracts

    Science.gov (United States)

    Irum, Bushra; Khan, Shahid Y.; Ali, Muhammad; Kaul, Haiba; Kabir, Firoz; Rauf, Bushra; Fatima, Fareeha; Nadeem, Raheela; Khan, Arif O.; Al Obaisi, Saif; Naeem, Muhammad Asif; Nasir, Idrees A.; Khan, Shaheen N.; Husnain, Tayyab; Riazuddin, Sheikh; Akram, Javed; Eghrari, Allen O.; Riazuddin, S. Amer

    2016-01-01

    Purpose To identify the molecular basis of non-syndromic autosomal recessive congenital cataracts (arCC) in a consanguineous family. Methods All family members participating in the study received a comprehensive ophthalmic examination to determine their ocular phenotype and contributed a blood sample, from which genomic DNA was extracted. Available medical records and interviews with the family were used to compile the medical history of the family. The symptomatic history of the individuals exhibiting cataracts was confirmed by slit-lamp biomicroscopy. A genome-wide linkage analysis was performed to localize the disease interval. The candidate gene, LIM2 (lens intrinsic membrane protein 2), was sequenced bi-directionally to identify the disease-causing mutation. The physical changes caused by the mutation were analyzed in silico through homology modeling, mutation and bioinformatic algorithms, and evolutionary conservation databases. The physiological importance of LIM2 to ocular development was assessed in vivo by real-time expression analysis of Lim2 in a mouse model. Results Ophthalmic examination confirmed the diagnosis of nuclear cataracts in the affected members of the family; the inheritance pattern and cataract development in early infancy indicated arCC. Genome-wide linkage analysis localized the critical interval to chromosome 19q with a two-point logarithm of odds (LOD) score of 3.25. Bidirectional sequencing identified a novel missense mutation, c.233G>A (p.G78D) in LIM2. This mutation segregated with the disease phenotype and was absent in 192 ethnically matched control chromosomes. In silico analysis predicted lower hydropathicity and hydrophobicity but higher polarity of the mutant LIM2-encoded protein (MP19) compared to the wild-type. Moreover, these analyses predicted that the mutation would disrupt the secondary structure of a transmembrane domain of MP19. The expression of Lim2, which was detected in the mouse lens as early as embryonic day 15

  13. An update on the LIM and SH3 domain protein 1 (LASP1): a versatile structural, signaling, and biomarker protein

    OpenAIRE

    2014-01-01

    The gene encoding the LIM and SH3 domain protein (LASP1) was cloned two decades ago from a cDNA library of breast cancer metastases. As the first protein of a class comprising one N-terminal LIM and one C-terminal SH3 domain, LASP1 founded a new LIM-protein subfamily of the nebulin group. Since its discovery LASP1 proved to be an extremely versatile protein because of its exceptional structure allowing interaction with various binding partners, its ubiquitous expression in normal tissues, alb...

  14. Asepsis Sowing and in vitro Propagation of Brassolaeliocattleya Sung Ya Green%卡特兰无菌播种与快速繁殖

    Institute of Scientific and Technical Information of China (English)

    郑宝强; 王雁; 彭镇华

    2010-01-01

    @@ 1 植物名称 卡特兰杂交种(Brassolaeliocattleya Sung Ya Green'Green World'). 2材料类别 人工自花授粉后90、120、150、180和210 d的种子. 3培养条件 种子萌发培养基:(1)KC;(2)MS;(3)1/2MS.原球茎诱导、增殖和分化培养基:(4)1/2MS+NAA 0.2 mg·L-1(单位下同);(5)1/2MS+NAA 0.4;(6)1/2MS+6-BA 0.5+NAA 0.2;(7)1/2MS+6-BA 1.0+NAA0.2.

  15. The Platform of Laboratory Information Management System (LIMS)%实验室信息管理系统(LIMS)的"运行平台"

    Institute of Scientific and Technical Information of China (English)

    王海芹

    2004-01-01

    阐述了分析测试实验室如何实施实验室信息管理系统(LIMS),论述影响LIMS成功应用的五个因素,包括实验室管理状况、实验室局域网建设、实验室管理层对LIMS实施的作用以及LIMS的应用需要实验室人员善于学习和合理地界定对LIMS应用效果的期望值.

  16. Comparison of horizontal winds from the LIMS satellite instrument with rocket measurements

    Science.gov (United States)

    Smith, A. K.; Bailey, P. L.

    1985-01-01

    Statistical results are given for a comparison between horizontal geostrophic winds computed from satellite height data and all available in situ rocket wind soundings during a 7-month period. The satellite data are the daily mapped fields from the Nimbus 7 Limb Infrared Monitor of the Stratosphere (LIMS) instrument, which extend from 100 to 0.1 mbar. Results indicate that in both the tropics and the extratropical Northern Hemisphere, the average zonal and meridional wind speeds agree to within 2-4 m/s throughout the stratosphere. The rms differences are much larger, with values of 5-10 m/s in the lower stratosphere, increasing to 20-40 m/s in the lower mesosphere. Time series show that LIMS and rocketsonde zonal wind speeds show coherent variations with temporal periods of 1-2 weeks and more, and both exhibit irregular variations on time scales of less than one week.

  17. Roles of LIM kinases in central nervous system function and dysfunction.

    Science.gov (United States)

    Cuberos, H; Vallée, B; Vourc'h, P; Tastet, J; Andres, C R; Bénédetti, H

    2015-12-21

    LIM kinase 1 (LIMK1) and LIM kinase 2 (LIMK2) regulate actin dynamics by phosphorylating cofilin. In this review, we outline studies that have shown an involvement of LIMKs in neuronal function and we detail some of the pathways and molecular mechanisms involving LIMKs in neurodevelopment and synaptic plasticity. We also review the involvement of LIMKs in neuronal diseases and emphasize the differences in the regulation of LIMKs expression and mode of action. We finally present the existence of a cofilin-independent pathway also involved in neuronal function. A better understanding of the differences between both LIMKs and of the precise molecular mechanisms involved in their mode of action and regulation is now required to improve our understanding of the physiopathology of the neuronal diseases associated with LIMKs.

  18. Insights into the molecular evolution of the PDZ/LIM family and identification of a novel conserved protein motif.

    Directory of Open Access Journals (Sweden)

    Aartjan J W Te Velthuis

    Full Text Available The PDZ and LIM domain-containing protein family is encoded by a diverse group of genes whose phylogeny has currently not been analyzed. In mammals, ten genes are found that encode both a PDZ- and one or several LIM-domains. These genes are: ALP, RIL, Elfin (CLP36, Mystique, Enigma (LMP-1, Enigma homologue (ENH, ZASP (Cypher, Oracle, LMO7 and the two LIM domain kinases (LIMK1 and LIMK2. As conventional alignment and phylogenetic procedures of full-length sequences fell short of elucidating the evolutionary history of these genes, we started to analyze the PDZ and LIM domain sequences themselves. Using information from most sequenced eukaryotic lineages, our phylogenetic analysis is based on full-length cDNA-, EST-derived- and genomic- PDZ and LIM domain sequences of over 25 species, ranging from yeast to humans. Plant and protozoan homologs were not found. Our phylogenetic analysis identifies a number of domain duplication and rearrangement events, and shows a single convergent event during evolution of the PDZ/LIM family. Further, we describe the separation of the ALP and Enigma subfamilies in lower vertebrates and identify a novel consensus motif, which we call 'ALP-like motif' (AM. This motif is highly-conserved between ALP subfamily proteins of diverse organisms. We used here a combinatorial approach to define the relation of the PDZ and LIM domain encoding genes and to reconstruct their phylogeny. This analysis allowed us to classify the PDZ/LIM family and to suggest a meaningful model for the molecular evolution of the diverse gene architectures found in this multi-domain family.

  19. ms_lims, a simple yet powerful open source laboratory information management system for MS-driven proteomics.

    Science.gov (United States)

    Helsens, Kenny; Colaert, Niklaas; Barsnes, Harald; Muth, Thilo; Flikka, Kristian; Staes, An; Timmerman, Evy; Wortelkamp, Steffi; Sickmann, Albert; Vandekerckhove, Joël; Gevaert, Kris; Martens, Lennart

    2010-03-01

    MS-based proteomics produces large amounts of mass spectra that require processing, identification and possibly quantification before interpretation can be undertaken. High-throughput studies require automation of these various steps, and management of the data in association with the results obtained. We here present ms_lims (http://genesis.UGent.be/ms_lims), a freely available, open-source system based on a central database to automate data management and processing in MS-driven proteomics analyses.

  20. MASTR-MS: a web-based collaborative laboratory information management system (LIMS) for metabolomics.

    Science.gov (United States)

    Hunter, Adam; Dayalan, Saravanan; De Souza, David; Power, Brad; Lorrimar, Rodney; Szabo, Tamas; Nguyen, Thu; O'Callaghan, Sean; Hack, Jeremy; Pyke, James; Nahid, Amsha; Barrero, Roberto; Roessner, Ute; Likic, Vladimir; Tull, Dedreia; Bacic, Antony; McConville, Malcolm; Bellgard, Matthew

    2017-01-01

    An increasing number of research laboratories and core analytical facilities around the world are developing high throughput metabolomic analytical and data processing pipelines that are capable of handling hundreds to thousands of individual samples per year, often over multiple projects, collaborations and sample types. At present, there are no Laboratory Information Management Systems (LIMS) that are specifically tailored for metabolomics laboratories that are capable of tracking samples and associated metadata from the beginning to the end of an experiment, including data processing and archiving, and which are also suitable for use in large institutional core facilities or multi-laboratory consortia as well as single laboratory environments. Here we present MASTR-MS, a downloadable and installable LIMS solution that can be deployed either within a single laboratory or used to link workflows across a multisite network. It comprises a Node Management System that can be used to link and manage projects across one or multiple collaborating laboratories; a User Management System which defines different user groups and privileges of users; a Quote Management System where client quotes are managed; a Project Management System in which metadata is stored and all aspects of project management, including experimental setup, sample tracking and instrument analysis, are defined, and a Data Management System that allows the automatic capture and storage of raw and processed data from the analytical instruments to the LIMS. MASTR-MS is a comprehensive LIMS solution specifically designed for metabolomics. It captures the entire lifecycle of a sample starting from project and experiment design to sample analysis, data capture and storage. It acts as an electronic notebook, facilitating project management within a single laboratory or a multi-node collaborative environment. This software is being developed in close consultation with members of the metabolomics research

  1. A possible role of LIM mineralization protein 1 in tertiary dentinogenesis of dental caries treatment.

    Science.gov (United States)

    Wang, Xiao-Ying; Zhang, Qi; Chen, Zhi

    2007-01-01

    Dental caries is a slowly progressive infectious disease with high risks. In response to dental caries stimuli, the tertiary dentin might be formed, protecting the dental pulp. Tertiary dentinogenesis contributes greatly to the treatment of carious lesions as well as the preservation and restoration of entire tooth function. A number of studies have found that application of exogenous growth factors such as transforming growth factors and bone morphogenetic proteins on unexposed pulps are able to signal tertiary dentinogenesis. Since precise mechanism of tertiary dentinogenesis is still not clear, more potential signaling factors might contribute to this process. Dentinogenesis shares many similarities with osteogenesis. The factors involved in osteogenesis and bone repair such as bone morphogenetic proteins 2, 7 and core binding factor alpha1 play important roles in dentinogenesis. LIM mineralization protein 1 is a critical positive regulator of osteoblast differentiation, bone formation and repair. It is logical to postulate that LIM mineralization protein 1 might be involved in odontoblast differentiation and dentin formation both in normal and in pathological conditions. Application of LIM mineralization protein 1 might be a promising approach for inducing tertiary dentinogenesis in dental caries treatment.

  2. Sulindac modulates secreted protein expression from LIM1215 colon carcinoma cells prior to apoptosis.

    Science.gov (United States)

    Greening, David W; Ji, Hong; Kapp, Eugene A; Simpson, Richard J

    2013-11-01

    Colorectal cancer (CRC) is a major cause of mortality in Western populations. Growing evidence from human and rodent studies indicate that nonsteroidal anti-inflammatory drugs (NSAIDs) cause regression of existing colon tumors and act as effective chemopreventive agents in sporadic colon tumor formation. Although much is known about the action of the NSAID sulindac, especially its role in inducing apoptosis, mechanisms underlying these effects is poorly understood. In previous secretome-based proteomic studies using 2D-DIGE/MS and cytokine arrays we identified over 150 proteins released from the CRC cell line LIM1215 whose expression levels were dysregulated by treatment with 1mM sulindac over 16h; many of these proteins are implicated in molecular and cellular functions such as cell proliferation, differentiation, adhesion, angiogenesis and apoptosis (Ji et al., Proteomics Clin. Appl. 2009, 3, 433-451). We have extended these studies and describe here an improved protein/peptide separation strategy that facilitated the identification of 987 proteins and peptides released from LIM1215 cells following 1mM sulindac treatment for 8h preceding the onset of apoptosis. This peptidome separation strategy involved fractional centrifugal ultrafiltration of concentrated cell culture media (CM) using nominal molecular weight membrane filters (NMWL 30K, 3K and 1K). Proteins isolated in the >30K and 3-30K fractions were electrophoretically separated by SDS-PAGE and endogenous peptides in the 1-3K membrane filter were fractioned by RP-HPLC; isolated proteins and peptides were identified by nanoLC-MS-MS. Collectively, our data show that LIM1215 cells treated with 1mM sulindac for 8h secrete decreased levels of proteins associated with extracellular matrix remodeling (e.g., collagens, perlecan, syndecans, filamins, dyneins, metalloproteinases and endopeptidases), cell adhesion (e.g., cadherins, integrins, laminins) and mucosal maintenance (e.g., glycoprotein 340 and mucins 5AC, 6

  3. Exploration of Chinese Allusions in Yi Kyu-bo's Fu——Centering on Yi's Wei Fu%朝鲜李奎报汉文赋引中国文化典故探析——以《畏赋》为中心

    Institute of Scientific and Technical Information of China (English)

    郭延红

    2012-01-01

    李奎报喜爱中国传统文化,对蕴涵在中国古代典籍中的典故十分了解,在他的赋作中,篇篇皆用典,所用典故类型丰富,意蕴深厚。典故的运用,使李奎报的赋更加含蓄、概括、凝练,更扩大了其赋的内涵。典故在李奎报赋文中被广泛运用,与他所处时代的政治、文化背景和他的个人修养等密切相关。%As a scholar in ancient Korea,Yi Kyu-bo was well-known for his works of Fu,a style of writing originated from China.Yi appreciated Chinese traditional cultures and had a good command of the allusions in Chinese ancient records.Various allusions with rich connotations were quoted and can be found in each of Yi's Fu.This enlarged the meaning of his Fu and made them more implicit,conclusive and concise.The extensive use of allusions relates closely with the political situations and cultural phenomenon at that time as well as Yi's self-cultivation.

  4. Role of the New South Wales Department of Primary Industries' Laboratory Information Management System (LIMS) in the 2007 equine influenza emergency animal disease response.

    Science.gov (United States)

    Croft, M G; Fraser, G C; Gaul, W N

    2011-07-01

    A Laboratory Information Management System (LIMS) was used to manage the laboratory data and support planning and field activities as part of the response to the equine influenza outbreak in Australia in 2007. The database structure of the LIMS and the system configurations that were made to best handle the laboratory implications of the disease response are discussed. The operational aspects of the LIMS and the related procedures used at the laboratory to process the increased sample throughput are reviewed, as is the interaction of the LIMS with other corporate systems used in the management of the response. Outcomes from this tailored configuration and operation of the LIMS resulted in effective provision and control of the laboratory and laboratory information aspects of the response. The extent and immediate availability of the information provided from the LIMS was critical to some of the activities of key operatives involved in controlling the response.

  5. adLIMS: a customized open source software that allows bridging clinical and basic molecular research studies.

    Science.gov (United States)

    Calabria, Andrea; Spinozzi, Giulio; Benedicenti, Fabrizio; Tenderini, Erika; Montini, Eugenio

    2015-01-01

    Many biological laboratories that deal with genomic samples are facing the problem of sample tracking, both for pure laboratory management and for efficiency. Our laboratory exploits PCR techniques and Next Generation Sequencing (NGS) methods to perform high-throughput integration site monitoring in different clinical trials and scientific projects. Because of the huge amount of samples that we process every year, which result in hundreds of millions of sequencing reads, we need to standardize data management and tracking systems, building up a scalable and flexible structure with web-based interfaces, which are usually called Laboratory Information Management System (LIMS). We started collecting end-users' requirements, composed of desired functionalities of the system and Graphical User Interfaces (GUI), and then we evaluated available tools that could address our requirements, spanning from pure LIMS to Content Management Systems (CMS) up to enterprise information systems. Our analysis identified ADempiere ERP, an open source Enterprise Resource Planning written in Java J2EE, as the best software that also natively implements some highly desirable technological advances, such as the high usability and modularity that grants high use-case flexibility and software scalability for custom solutions. We extended and customized ADempiere ERP to fulfil LIMS requirements and we developed adLIMS. It has been validated by our end-users verifying functionalities and GUIs through test cases for PCRs samples and pre-sequencing data and it is currently in use in our laboratories. adLIMS implements authorization and authentication policies, allowing multiple users management and roles definition that enables specific permissions, operations and data views to each user. For example, adLIMS allows creating sample sheets from stored data using available exporting operations. This simplicity and process standardization may avoid manual errors and information backtracking, features

  6. Phosphorylated LIM kinases colocalize with gamma-tubulin in centrosomes during early stages of mitosis.

    Science.gov (United States)

    Chakrabarti, Ratna; Jones, Jennifer L; Oelschlager, Denise K; Tapia, Tenekua; Tousson, Albert; Grizzle, William E

    2007-12-01

    LIM kinases (LIMK1 and LIMK2) are LIM domain containing serine/threonine kinases that modulate reorganization of actin cytoskeleton through inactivating phosphorylation of cofilin. The Rho family of small GTPases regulates the catalytic activity of LIMK1 and LIMK2 through activating phosphorylation by ROCK or by p21 kinase. Recent studies have suggested that LIMK1 could play a role in modulation of cellular growth by alteration of the cell cycle in breast and prostate tumor cells; however, the direct mitogenic effects of LIMK1 in these tumor cells is yet to be elucidated. Via immunofluorescence, in this study, we show that phosphorylated LIM kinases (pLIMK1/2) are colocalized with gamma-tubulin in the centrosomes during the early mitotic phases of human breast and prostate cancer cells (MDA-MB-231 and DU145); apparent colocalization begins in the centrosomes in prophase. As shown by both bright field (MDA-MB-231) and fluorescent immunohistochemistry (MDA-MB-231 and DU145), pLIMK1/2 does not localize to centrosomes during interphase. By bright field immunohistochemistry, the largest area of the centrosome that is stained with pLIMK1/2 occurs at anaphase. In early telophase, reduced staining of pLIMK1/2 at the spindle poles and concomitant accumulation of pLIMK1/2 at the cleavage furrow begins to occur. In late telophase, loss of staining of pLIMK1/2 and of colocalization with gamma-tubulin occurs at the poles and pLIMK1/2 became further concentrated at the junction between the two daughter cells. Co-immunoprecipitation studies indicated that gamma-tubulin associates with phosphorylated LIMK1 and LIMK2 but not with dephosphorylated LIMK1 or LIMK2. The results suggest that activated LIMK1/2 may associate with gamma-tubulin and play a role in mitotic spindle assembly.

  7. Interaction of subway LIM vehicle with ballasted track in polygonal wheel wear development

    Institute of Scientific and Technical Information of China (English)

    Ling Li; Xin-Biao Xiao; Xue-Song Jin

    2011-01-01

    This paper develops a coupled dynamics model for a linear induction motor (LIM) vehicle and a subway track to investigate the influence of polygonal wheels of the vehicle on the dynamic behavior of the system. In the model,the vehicle is modeled as a multi-body system with 35 degrees of freedom. A Timoshenko beam is used to model the rails which are discretely supported by sleepers. The sleepers are modeled as rigid bodies with their vertical, lateral,and rolling motions being considered. In order to simulate the vehicle running along the track, a moving sleeper support model is introduced to simulate the excitation by the discrete sleeper supporters, in which the sleepers are assumed to move backward at a constant speed that is the same as the train speed. The Hertzian contact theory and the ShenHedrick-Elkins' model are utilized to deal with the normal dynamic forces and the tangential forces between wheels and rails, respectively. In order to better characterize the linear metro system (LMS), Euler beam theory based on modal superposition method is used to model LIM and RP. The vertical electric magnetic force and the lateral restoring force between the LIM and RP are also taken into consideration.The former has gap-varying nonlinear characteristics, whilst the latter is considered as a constant restoring force of 1 kN.The numerical analysis considers the effect of the excitation due to polygonal wheels on the dynamic behavior of the system at different wear stages, in which the used data regarding the polygonal wear on the wheel tread are directly measured at the subway site.

  8. Interaction of subway LIM vehicle with ballasted track in polygonal wheel wear development

    Science.gov (United States)

    Li, Ling; Xiao, Xin-Biao; Jin, Xue-Song

    2011-04-01

    This paper develops a coupled dynamics model for a linear induction motor (LIM) vehicle and a subway track to investigate the influence of polygonal wheels of the vehicle on the dynamic behavior of the system. In the model, the vehicle is modeled as a multi-body system with 35 degrees of freedom. A Timoshenko beam is used to model the rails which are discretely supported by sleepers. The sleepers are modeled as rigid bodies with their vertical, lateral, and rolling motions being considered. In order to simulate the vehicle running along the track, a moving sleeper support model is introduced to simulate the excitation by the discrete sleeper supporters, in which the sleepers are assumed to move backward at a constant speed that is the same as the train speed. The Hertzian contact theory and the Shen-Hedrick-Elkins' model are utilized to deal with the normal dynamic forces and the tangential forces between wheels and rails, respectively. In order to better characterize the linear metro system (LMS), Euler beam theory based on modal superposition method is used to model LIM and RP. The vertical electric magnetic force and the lateral restoring force between the LIM and RP are also taken into consideration. The former has gap-varying nonlinear characteristics, whilst the latter is considered as a constant restoring force of 1 kN. The numerical analysis considers the effect of the excitation due to polygonal wheels on the dynamic behavior of the system at different wear stages, in which the used data regarding the polygonal wear on the wheel tread are directly measured at the subway site.

  9. Effects of polar stratospheric clouds in the Nimbus 7 LIMS Version 6 data set

    Science.gov (United States)

    Remsberg, Ellis; Harvey, V. Lynn

    2016-07-01

    The historic Limb Infrared Monitor of the Stratosphere (LIMS) measurements of 1978-1979 from the Nimbus 7 satellite were re-processed with Version 6 (V6) algorithms and archived in 2002. The V6 data set employs updated radiance registration methods, improved spectroscopic line parameters, and a common vertical resolution for all retrieved parameters. Retrieved profiles are spaced about every 1.6° of latitude along orbits and include the additional parameter of geopotential height. Profiles of O3 are sensitive to perturbations from emissions of polar stratospheric clouds (PSCs). This work presents results of implementing a first-order screening for effects of PSCs using simple algorithms based on vertical gradients of the O3 mixing ratio. Their occurrences are compared with the co-located, retrieved temperatures and related to the temperature thresholds needed for saturation of H2O and/or HNO3 vapor onto PSC particles. Observed daily locations where the major PSC screening criteria are satisfied are validated against PSCs observed with the Stratospheric Aerosol Monitor (SAM) II experiment also on Nimbus 7. Remnants of emissions from PSCs are characterized for O3 and HNO3 following the screening. PSCs may also impart a warm bias in the co-located LIMS temperatures, but by no more than 1-2 K at the altitudes of where effects of PSCs are a maximum in the ozone; thus, no PSC screening was applied to the V6 temperatures. Minimum temperatures vary between 187 and 194 K and often occur 1 to 2 km above where PSC effects are first identified in the ozone (most often between about 21 and 28 hPa). Those temperature-pressure values are consistent with conditions for the existence of nitric acid trihydrate (NAT) mixtures and to a lesser extent of super-cooled ternary solution (STS) droplets. A local, temporary uptake of HNO3 vapor of order 1-3 ppbv is indicated during mid-January for the 550 K surface. Seven-month time series of the distributions of LIMS O3 and HNO3 are shown

  10. Effects of Polar Stratospheric Clouds in the Nimbus 7 LIMS Version 6 Data Set

    Science.gov (United States)

    Remsberg, Ellis; Harvey, V. Lynn

    2016-01-01

    The historic Limb Infrared Monitor of the Stratosphere (LIMS) measurements of 1978-1979 from the Nimbus 7 satellite were re-processed with Version 6 (V6) algorithms and archived in 2002. The V6 data set employs updated radiance registration methods, improved spectroscopic line parameters, and a common vertical resolution for all retrieved parameters. Retrieved profiles are spaced about every 1.6 of latitude along orbits and include the additional parameter of geopotential height. Profiles of O3 are sensitive to perturbations from emissions of polar stratospheric clouds (PSCs). This work presents results of implementing a first-order screening for effects of PSCs using simple algorithms based on vertical gradients of the O3 mixing ratio. Their occurrences are compared with the co-located, retrieved temperatures and related to the temperature thresholds needed for saturation of H2O and/or HNO3 vapor onto PSC particles. Observed daily locations where the major PSC screening criteria are satisfied are validated against PSCs observed with the Stratospheric Aerosol Monitor (SAM) II experiment also on Nimbus 7. Remnants of emissions from PSCs are characterized for O3 and HNO3 following the screening. PSCs may also impart a warm bias in the co-located LIMS temperatures, but by no more than 1-2K at the altitudes of where effects of PSCs are a maximum in the ozone; thus, no PSC screening was applied to the V6 temperatures. Minimum temperatures vary between 187 and 194K and often occur 1 to 2 km above where PSC effects are first identified in the ozone (most often between about 21 and 28 hPa). Those temperature-pressure values are consistent with conditions for the existence of nitric acid trihydrate (NAT) mixtures and to a lesser extent of super-cooled ternary solution (STS) droplets. A local, temporary uptake of HNO3 vapor of order 1-3 ppbv is indicated during mid-January for the 550K surface. Seven-month time series of the distributions of LIMS O3 and HNO3 are shown based

  11. LIMS and Laboratory Management%LIMS与实验室管理

    Institute of Scientific and Technical Information of China (English)

    杨海鹰; 沈彤; 陆婉珍

    2002-01-01

    从应用的角度介绍了实验室信息管理系统(Laboratory Information Management Systems,LIMS)的基本功能及其在实验室管理与标准化论证中的应用,并结合LIMS技术实际开发和应用的经验,对国内相关实验室应用LIMS技术时可能遇到的困难提供了一些建议.

  12. Degradation of LIM domain-binding protein three during processing of Spanish dry-cured ham

    OpenAIRE

    2014-01-01

    Extensive proteolysis takes place during the processing of dry-cured ham due to the action of muscle peptidases. The aim of this work was to study the degradation of LIM domain binding protein 3 (LDB3), which is located at the Z-lines of the sarcomere, at different times during the Spanish dry-cured ham processing (2, 3.5, 5, 6.5, and 9 months). A total of 107 peptides have been identified by mass spectrometry, most of them generated from the first region of the protein sequence (position 1-9...

  13. A LIM Domain Protein from Tobacco Involved in Actin-Bundling and Histone Gene Transcription

    Institute of Scientific and Technical Information of China (English)

    Danièle Moes; Sabrina Gatti; Céline Hoffmann; Monika Dieterle; Flora Moreau; Katrin Neumann; Marc Schumacher

    2013-01-01

    The two LIM domain-containing proteins from plants (LIMs) typically exhibit a dual cytoplasmic-nuclear distribution,suggesting that,in addition to their previously described roles in actin cytoskeleton organization,they participate in nuclear processes.Using a south-western blot-based screen aimed at identifying factors that bind to plant histone gene promoters,we isolated a positive clone containing the tobacco LIM protein WLIM2 (NtWLIM2) cDNA.Using both green fluorescent protein (GFP) fusion-and immunology-based strategies,we provide clear evidence that NtWLIM2 localizes to the actin cytoskeleton,the nucleus,and the nucleolus.Interestingly,the disruption of the actin cytoskeleton by latrunculin B significantly increases NtWLIM2 nuclear fraction,pinpointing a possible novel cytoskeletal-nuclear crosstalk.Biochemical and electron microscopy experiments reveal the ability of NtWLIM2 to directly bind to actin filaments and to crosslink the latter into thick actin bundles.Electrophoretic mobility shift assays show that NtWLIM2 specifically binds to the conserved octameric cis-elements (Oct) of the Arabidopsis histone H4A748 gene promoter and that this binding largely relies on both LIM domains.Importantly,reporter-based experiments conducted in Arabidopsis and tobacco protoplasts confirm the ability of NtWLIM2 to bind to and activate the H4A748 gene promoter in live cells.Expression studies indicate the constitutive presence of NtWLIM2 mRNA and NtWLIM2 protein during tobacco BY-2 cell proliferation and cell cycle progression,suggesting a role of NtWLIM2 in the activation of basal histone gene expression.Interestingly,both live cell and in vitro data support NtWLIM2 di/oligomerization.We propose that NtWLIM2 functions as an actin-stabilizing protein,which,upon cytoskeleton remodeling,shuttles to the nucleus in order to modify gene expression.

  14. The C. elegans LIM homeobox gene lin-11 specifies multiple cell fates during vulval development.

    Science.gov (United States)

    Gupta, Bhagwati P; Wang, Minqin; Sternberg, Paul W

    2003-06-01

    LIM homeobox family members regulate a variety of cell fate choices during animal development. In C. elegans, mutations in the LIM homeobox gene lin-11 have previously been shown to alter the cell division pattern of a subset of the 2 degrees lineage vulval cells. We demonstrate multiple functions of lin-11 during vulval development. We examined the fate of vulval cells in lin-11 mutant animals using five cellular markers and found that lin-11 is necessary for the patterning of both 1 degrees and 2 degrees lineage cells. In the absence of lin-11 function, vulval cells fail to acquire correct identity and inappropriately fuse with each other. The expression pattern of lin-11 reveals dynamic changes during development. Using a temporally controlled overexpression system, we show that lin-11 is initially required in vulval cells for establishing the correct invagination pattern. This process involves asymmetric expression of lin-11 in the 2 degrees lineage cells. Using a conditional RNAi approach, we show that lin-11 regulates vulval morphogenesis. Finally, we show that LDB-1, a NLI/Ldb1/CLIM2 family member, interacts physically with LIN-11, and is necessary for vulval morphogenesis. Together, these findings demonstrate that temporal regulation of lin-11 is crucial for the wild-type vulval patterning.

  15. The Hawaiian Algal Database: a laboratory LIMS and online resource for biodiversity data

    Directory of Open Access Journals (Sweden)

    Sauvage Thomas

    2009-09-01

    Full Text Available Abstract Background Organization and presentation of biodiversity data is greatly facilitated by databases that are specially designed to allow easy data entry and organized data display. Such databases also have the capacity to serve as Laboratory Information Management Systems (LIMS. The Hawaiian Algal Database was designed to showcase specimens collected from the Hawaiian Archipelago, enabling users around the world to compare their specimens with our photographs and DNA sequence data, and to provide lab personnel with an organizational tool for storing various biodiversity data types. Description We describe the Hawaiian Algal Database, a comprehensive and searchable database containing photographs and micrographs, geo-referenced collecting information, taxonomic checklists and standardized DNA sequence data. All data for individual samples are linked through unique accession numbers. Users can search online for sample information by accession number, numerous levels of taxonomy, or collection site. At the present time the database contains data representing over 2,000 samples of marine, freshwater and terrestrial algae from the Hawaiian Archipelago. These samples are primarily red algae, although other taxa are being added. Conclusion The Hawaiian Algal Database is a digital repository for Hawaiian algal samples and acts as a LIMS for the laboratory. Users can make use of the online search tool to view and download specimen photographs and micrographs, DNA sequences and relevant habitat data, including georeferenced collecting locations. It is publicly available at http://algae.manoa.hawaii.edu.

  16. The Hawaiian Algal Database: a laboratory LIMS and online resource for biodiversity data.

    Science.gov (United States)

    Wang, Norman; Sherwood, Alison R; Kurihara, Akira; Conklin, Kimberly Y; Sauvage, Thomas; Presting, Gernot G

    2009-09-04

    Organization and presentation of biodiversity data is greatly facilitated by databases that are specially designed to allow easy data entry and organized data display. Such databases also have the capacity to serve as Laboratory Information Management Systems (LIMS). The Hawaiian Algal Database was designed to showcase specimens collected from the Hawaiian Archipelago, enabling users around the world to compare their specimens with our photographs and DNA sequence data, and to provide lab personnel with an organizational tool for storing various biodiversity data types. We describe the Hawaiian Algal Database, a comprehensive and searchable database containing photographs and micrographs, geo-referenced collecting information, taxonomic checklists and standardized DNA sequence data. All data for individual samples are linked through unique accession numbers. Users can search online for sample information by accession number, numerous levels of taxonomy, or collection site. At the present time the database contains data representing over 2,000 samples of marine, freshwater and terrestrial algae from the Hawaiian Archipelago. These samples are primarily red algae, although other taxa are being added. The Hawaiian Algal Database is a digital repository for Hawaiian algal samples and acts as a LIMS for the laboratory. Users can make use of the online search tool to view and download specimen photographs and micrographs, DNA sequences and relevant habitat data, including georeferenced collecting locations. It is publicly available at http://algae.manoa.hawaii.edu.

  17. Licuefacción de suelos y fenómenos asociados durante el Terremoto de Limón

    OpenAIRE

    Mora, Sergio; Yasuda, Susumu

    2011-01-01

    The April 22, 1991 Telire-Limón earthquake (Ms= 7.5) caused considerable damage in Costa Rica and Panamá. Close to were affected by soil liquefaction-related phenomena, which occured extensively in the the Caribbean lowlands of Limón province (Matina, Moín, Banano, Sixaola) in Costa Rica and Bocas del Toro (Changuinola, Almirante) in Panamá. The areas affected could be classified in three groups: l) Inland alluvial terraces along the rivers, 2) Sand bars and beaches, 3) Litoral sandy strips b...

  18. LIM and SH3 protein-1 modulates CXCR2-mediated cell migration.

    Directory of Open Access Journals (Sweden)

    Dayanidhi Raman

    Full Text Available BACKGROUND: The chemokine receptor CXCR2 plays a pivotal role in migration of neutrophils, macrophages and endothelial cells, modulating several biological responses such as angiogenesis, wound healing and acute inflammation. CXCR2 is also involved in pathogenesis of chronic inflammation, sepsis and atherosclerosis. The ability of CXCR2 to associate with a variety of proteins dynamically is responsible for its effects on directed cell migration or chemotaxis. The dynamic network of such CXCR2 binding proteins is termed as "CXCR2 chemosynapse". Proteomic analysis of proteins that co-immunoprecipitated with CXCR2 in neutrophil-like dHL-60 cells revealed a novel protein, LIM and SH3 protein 1 (LASP-1, binds CXCR2 under both basal and ligand activated conditions. LASP-1 is an actin binding cytoskeletal protein, involved in the cell migration. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrate that CXCR2 and LASP-1 co-immunoprecipitate and co-localize at the leading edge of migrating cells. The LIM domain of LASP-1 directly binds to the carboxy-terminal domain (CTD of CXCR2. Moreover, LASP-1 also directly binds the CTD of CXCR1, CXCR3 and CXCR4. Using a site-directed and deletion mutagenesis approach, Iso323-Leu324 of the conserved LKIL motif on CXCR2-CTD was identified as the binding site for LASP-1. Interruption of the interaction between CXCR2-CTD and LIM domain of LASP-1 by dominant negative and knock down approaches inhibited CXCR2-mediated chemotaxis. Analysis for the mechanism for inhibition of CXCR2-mediated chemotaxis indicated that LASP-1/CXCR2 interaction is essential for cell motility and focal adhesion turnover involving activation of Src, paxillin, PAK1, p130CAS and ERK1/2. CONCLUSIONS/SIGNIFICANCE: We demonstrate here for the first time that LASP-1 is a key component of the "CXCR2 chemosynapse" and LASP-1 interaction with CXCR2 is critical for CXCR2-mediated chemotaxis. Furthermore, LASP-1 also directly binds the CTD of CXCR1, CXCR3 and

  19. LIM and SH3 protein-1 modulates CXCR2-mediated cell migration.

    Science.gov (United States)

    Raman, Dayanidhi; Sai, Jiqing; Neel, Nicole F; Chew, Catherine S; Richmond, Ann

    2010-04-19

    The chemokine receptor CXCR2 plays a pivotal role in migration of neutrophils, macrophages and endothelial cells, modulating several biological responses such as angiogenesis, wound healing and acute inflammation. CXCR2 is also involved in pathogenesis of chronic inflammation, sepsis and atherosclerosis. The ability of CXCR2 to associate with a variety of proteins dynamically is responsible for its effects on directed cell migration or chemotaxis. The dynamic network of such CXCR2 binding proteins is termed as "CXCR2 chemosynapse". Proteomic analysis of proteins that co-immunoprecipitated with CXCR2 in neutrophil-like dHL-60 cells revealed a novel protein, LIM and SH3 protein 1 (LASP-1), binds CXCR2 under both basal and ligand activated conditions. LASP-1 is an actin binding cytoskeletal protein, involved in the cell migration. We demonstrate that CXCR2 and LASP-1 co-immunoprecipitate and co-localize at the leading edge of migrating cells. The LIM domain of LASP-1 directly binds to the carboxy-terminal domain (CTD) of CXCR2. Moreover, LASP-1 also directly binds the CTD of CXCR1, CXCR3 and CXCR4. Using a site-directed and deletion mutagenesis approach, Iso323-Leu324 of the conserved LKIL motif on CXCR2-CTD was identified as the binding site for LASP-1. Interruption of the interaction between CXCR2-CTD and LIM domain of LASP-1 by dominant negative and knock down approaches inhibited CXCR2-mediated chemotaxis. Analysis for the mechanism for inhibition of CXCR2-mediated chemotaxis indicated that LASP-1/CXCR2 interaction is essential for cell motility and focal adhesion turnover involving activation of Src, paxillin, PAK1, p130CAS and ERK1/2. We demonstrate here for the first time that LASP-1 is a key component of the "CXCR2 chemosynapse" and LASP-1 interaction with CXCR2 is critical for CXCR2-mediated chemotaxis. Furthermore, LASP-1 also directly binds the CTD of CXCR1, CXCR3 and CXCR4, suggesting that LASP-1 is a general mediator of CXC chemokine mediated chemotaxis

  20. Implementation of Close Loop Speed Control with VVVF Control and Slip Regulation on LIM

    Directory of Open Access Journals (Sweden)

    K. Aditya

    2014-04-01

    Full Text Available Open loop VVVF control has the disadvantage of low output torque when working at low frequency and poor speed precision at different load conditions.Various performance-improving schemes have been proposed for the basic VVVF control by compensating slips occurring in the low frequency range and slips caused by changing loads. Numerous papers have been published on the close loop speed control of rotary induction motor. In this paper a close loop speed control with VVVF control and slip regulation has been implemented for LIM based conveyor belt test Rig which compensates the disadvantages of traditional Volts/Hz control. SIMULINK results are presented to validate the effectiveness of proposed scheme.

  1. The LIM Protein Ajuba Restricts the Second Heart Field Progenitor Pool by Regulating Isl1 Activity

    Science.gov (United States)

    Witzel, Hagen R.; Jungblut, Benno; Choe, Chong Pyo; Crump, J. Gage; Braun, Thomas; Dobreva, Gergana

    2013-01-01

    SUMMARY Morphogenesis of the heart requires tight control of cardiac progenitor cell specification, expansion, and differentiation. Retinoic acid (RA) signaling restricts expansion of the second heart field (SHF), serving as an important morphogen in heart development. Here, we identify the LIM domain protein Ajuba as a crucial regulator of the SHF progenitor cell specification and expansion. Ajuba-deficient zebra-fish embryos show an increased pool of Isl1+ cardiac progenitors and, subsequently, dramatically increased numbers of cardiomyocytes at the arterial and venous poles. Furthermore, we show that Ajuba binds Isl1, represses its transcriptional activity, and is also required for autorepression of Isl1 expression in an RA-dependent manner. Lack of Ajuba abrogates the RA-dependent restriction of Isl1+ cardiac cells. We conclude that Ajuba plays a central role in regulating the SHF during heart development by linking RA signaling to the function of Isl1, a key transcription factor in cardiac progenitor cells. PMID:22771034

  2. The LIM protein Ajuba restricts the second heart field progenitor pool by regulating Isl1 activity.

    Science.gov (United States)

    Witzel, Hagen R; Jungblut, Benno; Choe, Chong Pyo; Crump, J Gage; Braun, Thomas; Dobreva, Gergana

    2012-07-17

    Morphogenesis of the heart requires tight control of cardiac progenitor cell specification, expansion, and differentiation. Retinoic acid (RA) signaling restricts expansion of the second heart field (SHF), serving as an important morphogen in heart development. Here, we identify the LIM domain protein Ajuba as a crucial regulator of the SHF progenitor cell specification and expansion. Ajuba-deficient zebrafish embryos show an increased pool of Isl1(+) cardiac progenitors and, subsequently, dramatically increased numbers of cardiomyocytes at the arterial and venous poles. Furthermore, we show that Ajuba binds Isl1, represses its transcriptional activity, and is also required for autorepression of Isl1 expression in an RA-dependent manner. Lack of Ajuba abrogates the RA-dependent restriction of Isl1(+) cardiac cells. We conclude that Ajuba plays a central role in regulating the SHF during heart development by linking RA signaling to the function of Isl1, a key transcription factor in cardiac progenitor cells.

  3. Degradation of LIM domain-binding protein three during processing of Spanish dry-cured ham.

    Science.gov (United States)

    Gallego, Marta; Mora, Leticia; Fraser, Paul D; Aristoy, María-Concepción; Toldrá, Fidel

    2014-04-15

    Extensive proteolysis takes place during the processing of dry-cured ham due to the action of muscle peptidases. The aim of this work was to study the degradation of LIM domain binding protein 3 (LDB3), which is located at the Z-lines of the sarcomere, at different times during the Spanish dry-cured ham processing (2, 3.5, 5, 6.5, and 9 months). A total of 107 peptides have been identified by mass spectrometry, most of them generated from the first region of the protein sequence (position 1-90) providing evidence for the complexity and variability of proteolytic reactions throughout the whole process of dry-curing. Methionine oxidation has been observed in several peptides by the end of the process. The potential of some of the identified peptides to be used as biomarkers of dry-cured ham processing has also been considered.

  4. Hoy hace: 13 años del Terremoto de Limón

    OpenAIRE

    Vallejos Vásquez, Sheily

    2005-01-01

    Se presenta una compilación de información referente al terremoto de Limón, ocurrido el día 22 de abril de 1991. Presenta algunos de los principales datos técnicos y de afectación. También explica los efectos producidos a nivel geológico y las acciones prioritarias que se realizaron. Por último, se expone la importancia que tuvo el sismo para la Comisión Nacional de Prevención de Riesgos y Atención de Emergencias y para la ejecución de iniciativas en la organización del país en el tema de los...

  5. LIM homeobox protein 5 (Lhx5) is essential for mamillary body development

    Science.gov (United States)

    Miquelajáuregui, Amaya; Sandoval-Schaefer, Teresa; Martínez-Armenta, Miriam; Pérez-Martínez, Leonor; Cárabez, Alfonso; Zhao, Yangu; Heide, Michael; Alvarez-Bolado, Gonzalo; Varela-Echavarría, Alfredo

    2015-01-01

    The mamillary body (MM) is a group of hypothalamic nuclei related to memory and spatial navigation that interconnects hippocampal, thalamic, and tegmental regions. Here we demonstrate that Lhx5, a LIM-HD domain transcription factor expressed early in the developing posterior hypothalamus, is required for the generation of the MM and its derived tracts. The MM markers Foxb1, Sim2, and Lhx1 are absent in Lhx5 knock-out mice from early embryonic stages, suggesting abnormal specification of this region. This was supported by the absence of Nkx2.1 and expansion of Shh in the prospective mamillary area. Interestingly, we also found an ectopic domain expressing Lhx2 and Lhx9 along the anterio-posterior hypothalamic axis. Our results suggest that Lhx5 controls early aspects of hypothalamic development by regulating gene expression and cellular specification in the prospective MM. PMID:26578897

  6. LIM homeobox protein 5 (Lhx5 is essential for mamillary body development

    Directory of Open Access Journals (Sweden)

    Amaya eMiquelajauregui

    2015-10-01

    Full Text Available The mamillary body (MM is a group of hypothalamic nuclei related to memory and spatial navigation that interconnects hippocampal, thalamic, and tegmental regions. Here we demonstrate that Lhx5, a LIM-HD domain transcription factor expressed early in the developing posterior hypothalamus, is required for the generation of the MM and its derived tracts. The MM markers Foxb1, Sim2 and Lhx1 are absent in Lhx5 knock-out mice from early embryonic stages, suggesting abnormal specification of this region. This was supported by the absence of Nkx2.1 and expansion of Shh in the prospective mamillary area. Interestingly, we also found an ectopic domain expressing Lhx2 and Lhx9 along the anterio-posterior hypothalamic axis. Our results suggest that Lhx5 controls early aspects of hypothalamic development by regulating gene expression and cellular specification in the prospective MM.

  7. Expression, purification and mass spectrometric analysis of LIM mineralization protein-1 in human lung epithelial cells

    Institute of Scientific and Technical Information of China (English)

    Sreedhara Sangadala; Louisa Titus; Scott D. Boden

    2008-01-01

    LIM mineralization protein-1 (LMP-1) is a novel osteoin ductive protein that has been cloned and shown to induce bone formation both in vitro and in vivo. Detection and evaluation of the possible presence of carbohydrate structures in LMP-1 is an important regulatory consideration for the therapeutic use of recombinantly expressed protein. The sequence of LMP-1 contains a highly conserved N-terminal PDZ domain and three C-terminal LIM domains. The sequence analysis of LMP-I predicts two potential N-glycosylation sites and several O-glycosylation sites. Here, we report the cloning and overexpression of LMP.1 in human lung carcinoma(A549) cells. Even though our group already reported the sequence of LMP-1 cDNA, we undertook this work to clarify whether or not the overexpressed protein undergoes any glycosylation in vivo. The expressed full-length recombinant protein was purified and subjected to chemical analysis and internal sequencing. The absence of any hexosamines (Nacetyl glucosamine or N-acetyl galactosamine) in chemical composition analysis of LMP.I protein revealed that there is little or no post-translational glycosylation of the LMP-1 polypeptide in lung carcinoma cells (A549). We performed in-gel trypsin digestion on purified LMP-I, and the resulting peptide digests were analyzed further using matrix.assisted laser desorption and ionization mass spectrometry for peptide mass finger printing, which produced several exact matches with the corresponding LMP-1 peptides. Separation by high performance liquid chromatography and purification of the desired peptides followed by N-terminal sequencing resulted in many exact LMP-1 matches for several purified peptides, thus establishing the identity of the purified protein as LMP-1.

  8. Paired and LIM class homeodomain proteins coordinate differentiation of the C. elegans ALA neuron.

    Science.gov (United States)

    Van Buskirk, Cheryl; Sternberg, Paul W

    2010-06-01

    The ancient origin of sleep is evidenced by deeply conserved signaling pathways regulating sleep-like behavior, such as signaling through the Epidermal growth factor receptor (EGFR). In Caenorhabditis elegans, a sleep-like state can be induced at any time during development or adulthood through conditional expression of LIN-3/EGF. The behavioral response to EGF is mediated by EGFR activity within a single cell, the ALA neuron, and mutations that impair ALA differentiation are expected to confer EGF-resistance. Here we describe three such EGF-resistant mutants. One of these corresponds to the LIM class homeodomain (HD) protein CEH-14/Lhx3, and the other two correspond to Paired-like HD proteins CEH-10/Chx10 and CEH-17/Phox2. Whereas CEH-14 is required for ALA-specific gene expression throughout development, the Prd-like proteins display complementary temporal contributions to gene expression, with the requirement for CEH-10 decreasing as that of CEH-17 increases. We present evidence that CEH-17 participates in a positive autoregulatory loop with CEH-14 in ALA, and that CEH-10, in addition to its role in ALA differentiation, functions in the generation of the ALA neuron. Similarly to CEH-17, CEH-10 is required for the posterior migration of the ALA axons, but CEH-14 appears to regulate an aspect of ALA axon outgrowth that is distinct from that of the Prd-like proteins. Our findings reveal partial modularity among the features of a neuronal differentiation program and their coordination by Prd and LIM class HD proteins.

  9. Transfer-Effizienz beim Übersetzen der humoristischen Stilelemente in der slowenischen Übersetzung von Kerstin Giers Roman "Für jede Lösung ein Problem"

    OpenAIRE

    2014-01-01

    Die vorliegende Magisterarbeit versuchte sich mit der Übersetzung des Humors am Beispiel des Kerstin Giers Romans „Für jede Lösung ein Problem“ auseinanderzusetzen und die Transfer-Effizienz der humoristischen Stilelemente in der slowenischen Übersetzung zu bewerten bzw. zu überprüfen. Die vorausgesetzte These lautete, dass die slowenische Übersetzung weniger humorvoll ist als das deutsche Original. Um die These zu bestätigen oder zu verwerfen, wurde eine Stilanalyse des Romans durchgeführt, ...

  10. El limón (Citrus limon Burman f.L) ingrediente natural en la elaboración de confituras de frutas de alta calidad

    OpenAIRE

    Sánchez-Campillo Sánchez, Pedro

    2012-01-01

    El objetivo concreto de la presente tesis doctoral es la aplicación de productos derivados del limón, aprovechando y transformando el subproducto que se genera en la industrialización del limón, en la elaboración de confituras de melocotón. Dadas las propiedades excepcionales y composición del limón, este fruto o parte de él, puede sustituir, total o parcialmente a aditivos químicos (pectinas, ácidos, antioxidantes…) en la elaboración de confituras de frutas, con los siguientes logros: se ...

  11. Estudo prospectivo da cadeia produtiva do capim-limão: estado do Paraná

    Directory of Open Access Journals (Sweden)

    Eliane Carneiro Gomes

    2004-12-01

    Full Text Available A aplicação de prospectiva para cadeias produtivas engloba várias etapas como a segmentação de cada elo, a análise do ambiente institucional e organizacional que envolve a cadeia, a identificação das necessidades e aspirações de cada segmento da cadeia e dos fatores críticos para a melhoria do desempenho. Desta forma, considerando a importância econômica da produção, industrialização e comercialização do capim-limão, Cymbopogon citratus (D.C. Stapf, e visando uma ação integrada do setor agrícola envolvido, este trabalho propõe o estudo prospectivo da cadeia produtiva desta espécie no estado do Paraná, identificando também fatores e condicionantes de qualidade em cada segmento desta cadeia. Para isto, o presente estudo apresenta o panorama da produção agroindustrial e da comunidade produtora agrícola paranaense de capim-limão; identifica e caracteriza os outros segmentos que integram a respectiva cadeia produtiva no Paraná e, por fim, identifica e analisa os principais gargalos, elos faltantes e pontos de estrangulamento. Evidenciaram-se como principais componentes da cadeia produtiva: a unidade produtiva, a agroindústria, a indústria (alimentícia, farmacêutica e química e o mercado consumidor interno (atacadista e varejista e externo. Com esse estudo prospectivo, espera-se contribuir para a implementação de medidas político-organizacionais, entre os agentes econômicos dos setores público e privado, que fazem parte da cadeia produtiva do capim-limão, possibilitando-lhes desenvolver estratégias que aumentem a competitividade e conduzam à expansão do mercado.The application of a prospective study to productive chains incorporates several stages like the segmentation of each link, the analysis of institutional and organizational environments to include the chain, the identification of necessities and aspirations of each chain segment and the critical factors for the improvement of performance. Thus

  12. Disc-shaped LIM for levitation and traction force control powered by the source using the component synchronous with the motor speed

    Directory of Open Access Journals (Sweden)

    Morizane Toshimitsu

    2015-12-01

    Full Text Available It has been proposed that a novel maglev transport system uses both of the attractive force and thrust force of the Linear Induction Motor (LIM. In our proposal, these two forces will be controlled by two different frequency components. One of the frequency components is synchronous with the motor speed (fm. Another frequency component is drive frequency (fd. Our proposed system enables the independent and simultaneous control of the attractive and thrust force of LIM. Each value of the attractive and the thrust force generated by fm and fd must be identified in order to design that LIM control system. For these purpose, a disc-shaped LIM has been developed as an experimental equipment. The force profiles, especially around zero slip, have been analyzed under experimental conditions.

  13. Uma visão psicobiológica da personalidade limítrofe Una visión psicobiológica de la personalidad limítrofe A psychobiological view of the borderline personality construct

    Directory of Open Access Journals (Sweden)

    Alan B. Eppel

    2005-12-01

    Full Text Available OBJETIVO: Revisar e reformular o conceito de distúrbio de personalidade limítrofe à luz dos avanços da neurociência e do desenvolvimento da infância. MÉTODO: A A base de dados Medline foi pesquisada utilizando-se as seguintes palavras-chave: personalidade limítrofe, regulação das emoções, neurobiologia, abuso sexual na infância, estabilizadores de humor. RESULTADOS: Existem predisposições genéticas a traços específicos de personalidade que parecem ser centrais aos conceitos de distúrbio de personalidade limítrofe. Esses traços combinados com relações de apego problemáticas e com abuso sexual na infância dão origem a vários efeitos conhecidos como distúrbio de personalidade limítrofe. CONCLUSÕES: O distúrbio de personalidade limítrofe é um distúrbio de regulação das emoções causado por fatores genéticos e interpessoais. IMPLICAÇÕES CLINICAS: As medidas preventivas deveriam ser direcionadas ao ambiente da primeira infância e à qualidade e disponibilidade de figuras de apego. O enfoque do tratamento deveria ser direcionado principalmente para a regulação das emoções, através de medicamentos, psicoterapia e treinamento de habilidades. Pesquisa básica a respeito da relação entre neuropeptídeos e serotonina poderiam levar a novas abordagens de intervenções psicofarmacológicas. LIMITAÇÕES: A pesquisa na área neurocientífica baseia-se principalmente em experimentos com animais e não podem ser totalmente extrapoladas para humanos. Há muito poucos ensaios clínicos randomizados controlados de antidepressivos e estabilizadores de humor em distúrbio de personalidade limítrofe. Devido à vastidão da literatura clínica e neurocientífica, essa revisão teve um enfoque selecionado.OBJETIVO: Revisar y reformular el concepto de disturbio de personalidad limítrofe a la luz de los avances de la neurociencia y del desarrollo de la niñez. MÉTODO: Se investigó la base de datos Medline, utiliz

  14. Numerical Simulations of the 1991 Limón Tsunami, Costa Rica Caribbean Coast

    Science.gov (United States)

    Chacón-Barrantes, Silvia; Zamora, Natalia

    2017-08-01

    The second largest recorded tsunami along the Caribbean margin of Central America occurred 25 years ago. On April 22nd, 1991, an earthquake with magnitude Mw 7.6 ruptured along the thrust faults that form the North Panamá Deformed Belt (NPDB). The earthquake triggered a tsunami that affected the Caribbean coast of Costa Rica and Panamá within few minutes, generating two casualties. These are the only deaths caused by a tsunami in Costa Rica. Coseismic uplift up to 1.6 m and runup values larger than 2 m were measured along some coastal sites. Here, we consider three solutions for the seismic source as initial conditions to model the tsunami, each considering a single rupture plane. We performed numerical modeling of the tsunami propagation and runup using NEOWAVE numerical model (Yamazaki et al. in Int J Numer Methods Fluids 67:2081-2107, 2010, doi: 10.1002/fld.2485 ) on a system of nested grids from the entire Caribbean Sea to Limón city. The modeled surface deformation and tsunami runup agreed with the measured data along most of the coastal sites with one preferred model that fits the field data. The model results are useful to determine how the 1991 tsunami could have affected regions where tsunami records were not preserved and to simulate the effects of the coastal surface deformations as buffer to tsunami. We also performed tsunami modeling to simulate the consequences if a similar event with larger magnitude Mw 7.9 occurs offshore the southern Costa Rican Caribbean coast. Such event would generate maximum wave heights of more than 5 m showing that Limón and northwestern Panamá coastal areas are exposed to moderate-to-large tsunamis. These simulations considering historical events and maximum credible scenarios can be useful for hazard assessment and also as part of studies leading to tsunami evacuation maps and mitigation plans, even when that is not the scope of this paper.

  15. 棉花LIM蛋白基因家族的进化及表达特征分析%Evolution and Expression Analysis of the LIM Protein Gene Family in Cotton

    Institute of Scientific and Technical Information of China (English)

    杨洋; 李波; 胡文冉; 张经博; 范玲

    2015-01-01

    植物LIM蛋白参与细胞骨架的调节及调控次生细胞壁的发育。本研究在雷蒙德氏棉(D5)、亚洲棉(A2)基因组和陆地棉的A、D亚基因组中分别鉴定出14个LIM基因。陆地棉GhLIM基因在2个亚组间的染色体上的分布及基因的复制模式是一致的。推测其复制发生在其祖先同可可(6个LIM)分开之后, A2、D5基因组分化之前;陆地棉LIM除多倍体化使其数目加倍外,没有基因复制与丢失事件发生。蛋白系统进化分析表明,所有被子植物LIM蛋白可以划分为4个亚组(部分具有小亚组):αLIM1(FLIM1、WLIM1、PLIM1)、βLIM1、γLIM2(WLIM2)、δLIM2(PLIM2-I、PLIM2-II)。陆地棉GhLIM的同源复制基因分别在WLIM2和PLIM2-I、PLIM2-II中聚为独立的分支。通过分析深度测序表达谱,发现陆地棉GhLIM有3种表达类型:组成型表达的GhLIM1、2(FLIM), GhLIM3(WLIM1), GhLIM13(βLIM1)和GhLIM4、8、14(WLIM2);雄蕊中优势表达的GhLIM7、9(PLIM2-I), GhLIM5、6、10、11(PLIM2-II);在雄蕊和纤维中优势表达的GhLIM12(PLIM2-I)。实时荧光定量PCR分析表明, GhLIM基因各成员在不同的纤维发育阶段具有不同的表达特征, GhLIM1、13、14在纤维的快速伸长期(5~15 DPA)上调表达,在15 DPA表达量最高; GhLIM2则在纤维次生壁发育期(18~28 DPA)显著上调表达,在28 DPA表达量最高。而GhLIM12在10~23 DPA持续高表达,在28 DPA则没有表达。上述结果说明GhLIM家族基因在纤维发育的不同阶段发挥着不同的功能。%Plant LIM acts as an actin bundler to facilitate elongation of cells and also functions as a transcription factor involved in secondary cell wall development. In this study, 14 putative LIM genes were identified from the whole genomes of Gossypium raimondii (D5), G. arboreum (A2), and 2 subgenomes (A, D) of G. hirsutum, respectively. In G. hirsutum, the distribution and the duplication patterns of LIM family members on each couple

  16. Results from the LIMS experiment for the PMP-1 winter 1978/79. [Limb Infrared Monitor of the Stratosphere

    Science.gov (United States)

    Gille, J. C.; Bailey, P. L.; Lyjak, L. V.; Russell, J. M., III

    1982-01-01

    The results of a preliminary analysis of the data on the global distributions of temperature and trace constituents in the middle atmosphere (15-65 km) obtained with the limb IR monitor (LIMS) are presented. The 6-channel limb-scanning radiometer was sensitive to the 15 micron bands of CO2, as well as other wavelengths, and was launched on the Nimbus 7 satellite in 1978. The derived data were partially compared with Soviet rocketsonde data to track an evolution in a warming trend over Siberia. The morphology of the changes were attributed to stratospheric warming. The comparisons with rocketsonde data indicated that the LIMS data were suitable for the determination of planetary wave amplitudes and phases, heat and momentum transport, and Eliassen-Palm fluxes.

  17. Overexpression of small heat shock protein LimHSP16.45 in Arabidopsis enhances tolerance to abiotic stresses.

    Directory of Open Access Journals (Sweden)

    Changjun Mu

    Full Text Available Small heat shock proteins (smHSPs play important and extensive roles in plant defenses against abiotic stresses. We cloned a gene for a smHSP from the David Lily (Lilium davidii (E. H. Wilson Raffill var. Willmottiae, which we named LimHSP16.45 based on its protein molecular weight. Its expression was induced by many kinds of abiotic stresses in both the lily and transgenic plants of Arabidopsis. Heterologous expression enhanced cell viability of the latter under high temperatures, high salt, and oxidative stress, and heat shock granules (HSGs formed under heat or salinity treatment. Assays of enzymes showed that LimHSP16.45 overexpression was related to greater activity by superoxide dismutase and catalase in transgenic lines. Therefore, we conclude that heterologous expression can protect plants against abiotic stresses by preventing irreversible protein aggregation, and by scavenging cellular reactive oxygen species.

  18. LIMS的历史、发展与实施应用%History, Development and Implementation of LIMS

    Institute of Scientific and Technical Information of China (English)

    北京英普思软件有限责任公司

    2002-01-01

    本论文内容涉及LIMS发展的简要历史及其应用、优点和核心功能,同时包含帮助第一时间用户成功实施LIMS的一些提示.%The paper covers a brief history of the development of LIMS, its uses, benefits, and core functions. Hints for successful implementation are also included to assist the first time user.

  19. cDNA Cloning, Sequence Analysis of the Porcine LIM and Cysteine-rich Domain 1 Gene

    Institute of Scientific and Technical Information of China (English)

    Jun WANG; Chang-Yan DENG; Yuan-Zhu XIONG; Bo ZUO; Lei XING; Feng-E LI; Ming-Gang LEI; Rong ZHENG; Si-Wen JIANG

    2005-01-01

    LIM domain proteins are important regulators in cell growth, cell fate determination, cell differentiation and remodeling of the cell cytoskeleton by their interaction with various structural proteins, kinases and transcriptional regulators. Using molecular biology combined with in silico cloning, we have cloned the complete coding sequence of pig LIM and the cysteine-rich domain 1 gene (LMCD1) which encodes a 363 amino acid protein. The estimated molecular weight of the LMCD1 protein is 40,788 Da with a pI of 8.39. It was found to be highly expressed in both skeletal muscle and cardiac muscle. Alignment analysis revealed that the deduced protein sequence shares 86%, 91% and 93% homology with that of its human, mouse and rat counterparts, respectively. The LMCD1 protein was predicted by bioinformatics software to contain a novel cysteine-rich domain in the N-terminal region, two LIM domains in the C-terminal region, nine potential protein kinase C phosphorylation sites, seven casein kinase Ⅱ phosphorylation sites, a tyrosine kinase phosphorylation site, seven N-glycosylation and N-myristoylation sites and a single potential N-glycosylation site, which is similar to the protein's human counterpart. Phylogenetic tree was constructed by aligning the amino acid sequences of the LIM domain from different species. In addition, four base mutations were detected by comparing the sequences of Large White pigs with those of Chinese Meishan pigs. The G294A mutation site was confirmed by polymerase chain reaction-single-strand conformation polymorphism analysis. Its allele frequencies were studied in five pig breeds.

  20. Analisis Intensitas Pencahayaan Dan Reflektan Di Lingkungan Kerja Terhadap Kelelahan Mata Pada Tenaga Kerja Industri Bulu Mata Palsu Di PT. Sung Shim International Purbalingga.

    Directory of Open Access Journals (Sweden)

    Feriyandi Feriyandi

    2015-12-01

    Full Text Available ABSTRACT Background : There are approximately 15,000 workers in several spurious eyelash industries in Purbalingga Regency. Since objects being observed are very fine and the tasks need high accuracy, good lighting is therefore highly required. Poor lighting can lead to visual and mental fatigue, stiffness around the eyes, eye damage and the increasing of work accidents. Sufficient intensity and good management of lighting will result a comfort environment in accordance  to achieve a safe  work environment. Method : This research was conducted as  observational study with a cross sectional approach  for 45 samples of workers. Sampling was carried out by using of systematic random sampling. Data of light intensity and reflectance were collected by measurement of light using Lux Meter and assessment of visual fatigue by using Retention Timer. Other data were collected by interviewing workers with age limitation up to 33 years old. Chi Square Test was used to analyze the data of light intensity and reflectance, work years, age, work period, distance of light to object, distance of eyes to object, relative humidity and temperature, and the correlation with the rise of visual fatigue. Result : Light intensity in the knitting section was between 301.5 – 576.7 lux with reflectance of 43.1%-89.6%. Thirty wokers (66.7% suffered from mild visual fatigue and fifteen wokers, suffered (33.3% from moderate visual fatigue. Light intensity had  an influence and significant relationship  to the occurring of visual fatigue among workers in the industry of spurious eyelash of  PT. Sung Shim International  (p < 0,05 with  Odd ratio = 10.246 (95% CI : 1.147 - 91.498.  Probability of light intensity factor to cause visual fatigue measured by a regression logistic model  was  82.9% . It was caused by  poor lighting (light intensities were measured  below 500 lux while 17.1%  was caused by  other factors. Conclusion : Most of the spurious eyelash

  1. The Cytotoxic Effect of Essential Oil of Syrian Citrus limon Peel on Human Colorectal Carcinoma Cell Line (Lim1863

    Directory of Open Access Journals (Sweden)

    Mohammad Eyad Chatty

    2012-01-01

    Full Text Available Background: Essential oils are the volatile fraction of aromatic and medicinal plants created after extraction by steam or water distillation. Species of the genus Citrus(Rutaceae have been widely used in traditional medicine as volatile oils and are currently the subject of numerous research. Citrus essential oil consists of different terpens that have antitumor activities. This study determines the cytotoxic effect of the essential oils of Citrus limon L. peels on a colorectal cancer cell line (LIM1863.Methods: We harvested four samples from four locations in Syria. Essential oils were prepared by hydrodistillation and analyzed by Gas chromatography-mass spectrometry (GC-MS.Various concentrations ofessential oils (0.5-48 μg/ml were added to cultured cells and incubated for 72 h. Cell viability was evaluated byMTT-basedcytotoxicity assay.Results: We noted 18 components that represented 98.81% of the total oil content. The major components were: limonene (61.8%-73.8%, γ-terpinene (9.4%-10.4%, β-pinene (3.7%-6.9%, O-cymene(1%-2.4%,and citral (0.8%-5.4%.The obtained IC50 value range of Citrus limon essential oils was 5.75-7.92 μg/ml against LIM1863.Conclusion: This study revealed that Syrian Citrus limon essential oil has a cytotoxic effect on the human colorectalcarcinoma cell line LIM1863 when studied in vitro.

  2. Characterization of the interaction between Actinin-Associated LIM Protein (ALP and the rod domain of α-actinin

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    Permi Perttu

    2009-03-01

    Full Text Available Abstract Background The PDZ-LIM proteins are a family of signalling adaptors that interact with the actin cross-linking protein, α-actinin, via their PDZ domains or via internal regions between the PDZ and LIM domains. Three of the PDZ-LIM proteins have a conserved 26-residue ZM motif in the internal region, but the structure of the internal region is unknown. Results In this study, using circular dichroism and nuclear magnetic resonance (NMR, we showed that the ALP internal region (residues 107–273 was largely unfolded in solution, but was able to interact with the α-actinin rod domain in vitro, and to co-localize with α-actinin on stress fibres in vivo. NMR analysis revealed that the titration of ALP with the α-actinin rod domain induces stabilization of ALP. A synthetic peptide (residues 175–196 that contained the N-terminal half of the ZM motif was found to interact directly with the α-actinin rod domain in surface plasmon resonance (SPR measurements. Short deletions at or before the ZM motif abrogated the localization of ALP to actin stress fibres. Conclusion The internal region of ALP appeared to be largely unstructured but functional. The ZM motif defined part of the interaction surface between ALP and the α-actinin rod domain.

  3. Deficiency in the LIM-only protein Fhl2 impairs skin wound healing.

    Science.gov (United States)

    Wixler, Viktor; Hirner, Stephanie; Müller, Judith M; Gullotti, Lucia; Will, Carola; Kirfel, Jutta; Günther, Thomas; Schneider, Holm; Bosserhoff, Anja; Schorle, Hubert; Park, Jung; Schüle, Roland; Buettner, Reinhard

    2007-04-09

    After skin wounding, the repair process is initiated by the release of growth factors, cytokines, and bioactive lipids from injured vessels and coagulated platelets. These signal molecules induce synthesis and deposition of a provisional extracellular matrix, as well as fibroblast invasion into and contraction of the wounded area. We previously showed that sphingosine-1-phosphate (S1P) triggers a signal transduction cascade mediating nuclear translocation of the LIM-only protein Fhl2 in response to activation of the RhoA GTPase (Muller, J.M., U. Isele, E. Metzger, A. Rempel, M. Moser, A. Pscherer, T. Breyer, C. Holubarsch, R. Buettner, and R. Schule. 2000. EMBO J. 19:359-369; Muller, J.M., E. Metzger, H. Greschik, A.K. Bosserhoff, L. Mercep, R. Buettner, and R. Schule. 2002. EMBO J. 21:736-748.). We demonstrate impaired cutaneous wound healing in Fhl2-deficient mice rescued by transgenic expression of Fhl2. Furthermore, collagen contraction and cell migration are severely impaired in Fhl2-deficient cells. Consequently, we show that the expression of alpha-smooth muscle actin, which is regulated by Fhl2, is reduced and delayed in wounds of Fhl2-deficient mice and that the expression of p130Cas, which is essential for cell migration, is reduced in Fhl2-deficient cells. In summary, our data demonstrate a function of Fhl2 as a lipid-triggered signaling molecule in mesenchymal cells regulating their migration and contraction during cutaneous wound healing.

  4. Screensaver: an open source lab information management system (LIMS for high throughput screening facilities

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    Nale Jennifer

    2010-05-01

    Full Text Available Abstract Background Shared-usage high throughput screening (HTS facilities are becoming more common in academe as large-scale small molecule and genome-scale RNAi screening strategies are adopted for basic research purposes. These shared facilities require a unique informatics infrastructure that must not only provide access to and analysis of screening data, but must also manage the administrative and technical challenges associated with conducting numerous, interleaved screening efforts run by multiple independent research groups. Results We have developed Screensaver, a free, open source, web-based lab information management system (LIMS, to address the informatics needs of our small molecule and RNAi screening facility. Screensaver supports the storage and comparison of screening data sets, as well as the management of information about screens, screeners, libraries, and laboratory work requests. To our knowledge, Screensaver is one of the first applications to support the storage and analysis of data from both genome-scale RNAi screening projects and small molecule screening projects. Conclusions The informatics and administrative needs of an HTS facility may be best managed by a single, integrated, web-accessible application such as Screensaver. Screensaver has proven useful in meeting the requirements of the ICCB-Longwood/NSRB Screening Facility at Harvard Medical School, and has provided similar benefits to other HTS facilities.

  5. Screensaver: an open source lab information management system (LIMS) for high throughput screening facilities

    Science.gov (United States)

    2010-01-01

    Background Shared-usage high throughput screening (HTS) facilities are becoming more common in academe as large-scale small molecule and genome-scale RNAi screening strategies are adopted for basic research purposes. These shared facilities require a unique informatics infrastructure that must not only provide access to and analysis of screening data, but must also manage the administrative and technical challenges associated with conducting numerous, interleaved screening efforts run by multiple independent research groups. Results We have developed Screensaver, a free, open source, web-based lab information management system (LIMS), to address the informatics needs of our small molecule and RNAi screening facility. Screensaver supports the storage and comparison of screening data sets, as well as the management of information about screens, screeners, libraries, and laboratory work requests. To our knowledge, Screensaver is one of the first applications to support the storage and analysis of data from both genome-scale RNAi screening projects and small molecule screening projects. Conclusions The informatics and administrative needs of an HTS facility may be best managed by a single, integrated, web-accessible application such as Screensaver. Screensaver has proven useful in meeting the requirements of the ICCB-Longwood/NSRB Screening Facility at Harvard Medical School, and has provided similar benefits to other HTS facilities. PMID:20482787

  6. Hoy hace: 13 años del Terremoto de Limón

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    Vallejos Vásquez, Sheily

    2005-08-01

    Full Text Available Se presenta una compilación de información referente al terremoto de Limón, ocurrido el día 22 de abril de 1991. Presenta algunos de los principales datos técnicos y de afectación. También explica los efectos producidos a nivel geológico y las acciones prioritarias que se realizaron. Por último, se expone la importancia que tuvo el sismo para la Comisión Nacional de Prevención de Riesgos y Atención de Emergencias y para la ejecución de iniciativas en la organización del país en el tema de los desastres It presents a compilation of the information in regards to the Limon earthquake, April 22, 1991. It presents some of the main technical and affectation data. It also explains the geological effects and the main actions taken. Lastly, it exposes the importance that the earthquake had for the National Commission of Risk Prevention and Emergency Response and for the execution of initiatives in the country organization in the topic of disasters

  7. The LIM-only protein FHL2 attenuates lung inflammation during bleomycin-induced fibrosis.

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    Abdulaleem Alnajar

    Full Text Available Fibrogenesis is usually initiated when regenerative processes have failed and/or chronic inflammation occurs. It is characterised by the activation of tissue fibroblasts and dysregulated synthesis of extracellular matrix proteins. FHL2 (four-and-a-half LIM domain protein 2 is a scaffolding protein that interacts with numerous cellular proteins, regulating signalling cascades and gene transcription. It is involved in tissue remodelling and tumour progression. Recent data suggest that FHL2 might support fibrogenesis by maintaining the transcriptional expression of alpha smooth muscle actin and the excessive synthesis and assembly of matrix proteins in activated fibroblasts. Here, we present evidence that FHL2 does not promote bleomycin-induced lung fibrosis, but rather suppresses this process by attenuating lung inflammation. Loss of FHL2 results in increased expression of the pro-inflammatory matrix protein tenascin C and downregulation of the macrophage activating C-type lectin receptor DC-SIGN. Consequently, FHL2 knockout mice developed a severe and long-lasting lung pathology following bleomycin administration due to enhanced expression of tenascin C and impaired activation of inflammation-resolving macrophages.

  8. Pardeamiento de zumos clarificados de limón tratados a altas temperaturas

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    Raquel Ibarz-Martínez

    2010-01-01

    Full Text Available En este trabajo se presenta un estudio del efecto del tratamiento a altas temperaturas (70, 80, 90 y 95ºC sobre la evolución del color de zumos clarificados de limón de 10, 20, 35, 50 y 64.6ºBrix. La evolución del color con el tiempo de tratamiento se ha seguido midiendo la absorbancia a 420 nm (A420 y los parámetros CIELab (Luminosidad L*, a* y b* e incremento de color E*. El aumento de A420 y la disminución de la luminosidad L* con el tiempo de tratamiento se ha observado que se ajustan a cinéticas de orden cero, mientras que E* evoluciona según una cinética combinada en dos etapas. El efecto de la temperatura sobre las constantes cinéticas se puede cuantificar mediante la ecuación de Arrhenius, obteniendo que las energías de activación, para la evolución de A420 y L*, muestran una tendencia a disminuir con el aumento de la concentración, mientras que para E* apenas existe variación. Para los tratamientos a una temperatura determinada, el efecto del contenido en sólidos solubles sobre las constantes cinéticas puede describirse mediante un modelo tipo exponencial.

  9. Muscle LIM protein deficiency leads to alterations in passive ventricular mechanics.

    Science.gov (United States)

    Omens, Jeffrey H; Usyk, Taras P; Li, Zuangjie; McCulloch, Andrew D

    2002-02-01

    Accumulating evidence indicates that cytoskeletal defects may be an important pathway for dilated cardiomyopathy and eventual heart failure. Targeted disruption of muscle LIM protein (MLP) has previously been shown to result in dilated cardiomyopathy with many of the clinical signs of heart failure, although the effects of MLP disruption on passive ventricular mechanics and myocyte architecture are not known. We used the MLP knockout model to examine changes in passive ventricular mechanics and laminar myofiber sheet architecture. Pressure-volume and pressure-strain relations were altered in MLP knockout mice, in general suggesting a less compliant tissue in the dilated hearts. Transmural laminar myocyte structure was also altered in this mouse model, especially near the epicardium. A mathematical model of the heart showed a likely increase in passive tissue stiffness in the MLP-deficient (-/-) heart. These results suggest that the disruption of the cytoskeletal protein MLP results in less compliant passive tissue and concomitant structural alterations in the three-dimensional myocyte architecture that may in part explain the ventricular dysfunction in the dilated heart.

  10. Improvements in the profiles and distributions of nitric acid and nitrogen dioxide with the LIMS version 6 dataset

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    E. Remsberg

    2010-05-01

    Full Text Available The quality of the Nimbus 7 Limb Infrared Monitor of the Stratosphere (LIMS nitric acid (HNO3 and nitrogen dioxide (NO2 profiles and distributions of 1978/1979 are described after their processing with an updated, Version 6 (V6 algorithm and subsequent archival in 2002. Estimates of the precision and accuracy of both of those species are developed and provided herein. The character of the V6 HNO3 profiles is relatively unchanged from that of the earlier LIMS Version 5 (V5 profiles, except in the upper stratosphere where the interfering effects of CO2 are accounted for better with V6. The accuracy of the retrieved V6 NO2 is also significantly better in the middle and upper stratosphere, due to improvements in its spectral line parameters and in the reduced biases for the accompanying V6 temperature and water vapor profiles. As a result of these important updates, there is better agreement with theoretical calculations for profiles of the HNO3/NO2 ratio, day-to-night NO2 ratio, and with estimates of the production of NO2 in the mesosphere and its descent to the upper stratosphere during polar night. In particular, the findings for middle and upper stratospheric NO2 should also be more compatible with those obtained from more recent satellite sensors because the effects of the spin-splitting of the NO2 lines are accounted for now with the LIMS V6 algorithm. The improved precisions and more frequent retrievals of the LIMS profiles along their orbit tracks provide for better continuity and detail in map analyses of these two species on pressure surfaces. It is judged that the chemical effects of the oxides of nitrogen on ozone can be studied quantitatively throughout the stratosphere with the LIMS V6 data.

  11. Expression of the Lhx genes apterous and lim1 in an errant polychaete: implications for bilaterian appendage evolution, neural development, and muscle diversification

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    Winchell Christopher J

    2013-02-01

    Full Text Available Abstract Background Arthropod and vertebrate appendages appear to have evolved via parallel co-option of a plesiomorphic gene regulatory network. Our previous work implies that annelids evolved unrelated appendage-forming mechanisms; we therefore found no support for homology of parapodia and arthropodia at the level of the whole appendage. We expand on that study here by asking whether expression of the LIM homeobox (Lhx genes apterous and lim1 in the annelid Neanthes arenaceodentata supports homology of the dorsal branches as well as the proximodistal axes of parapodia and arthropodia. In addition, we explore whether the neural expression of apterous and lim1 in Neanthes supports the putative ancestral function of the Lhx gene family in regulating the differentiation and maintenance of neuronal subtypes. Results Both genes exhibit continuous expression in specific portions of the developing central nervous system, from hatching to at least the 13-chaetiger stage. For example, nerve cord expression occurs in segmentally iterated patterns consisting of diffuse sets of many lim1-positive cells and comparatively fewer, clustered pairs of apterous-positive cells. Additionally, continuous apterous expression is observed in presumed neurosecretory ganglia of the posterior brain, while lim1 is continuously expressed in stomatogastric ganglia of the anterior brain. apterous is also expressed in the jaw sacs, dorsal parapodial muscles, and a presumed pair of cephalic sensory organs, whereas lim1 is expressed in multiple pharyngeal ganglia, the segmental peripheral nervous system, neuropodial chaetal sac muscles, and parapodial ligules. Conclusions The early and persistent nervous system expression of apterous and lim1 in Neanthes juveniles supports conservation of Lhx function in bilaterian neural differentiation and maintenance. Our results also suggest that diversification of parapodial muscle precursors involves a complementary LIM code similar to

  12. Shallow electrical resistivity imaging of the Limón fault, Chagres River Watershed, Panama Canal

    Science.gov (United States)

    Mojica, Alexis; Pérez, Tatiana; Toral, Jaime; Miranda, Roberto; Franceschi, Pastora; Calderón, Carlos; Vergara, Fidedigna

    2017-03-01

    The aim of this study was the use of electrical resistivity imaging to investigate the geometry of the southwest portion of one of the most important geologic fault zones of the Panama Canal Watershed: the Limón fault. This fault is characterized by its juxtaposition of pre-Tertiary andesitic basalt (Playa Venado Formation) against late Oligocene Tertiary sediments (Caimito Formation). In this zone, four 2D electrical resistivity tomography profiles were conducted perpendicular to the fault trace: T-1, T-2, T-3 and T-4. The T-1, T-3, and T-4 profiles were long profiles (235 m for the first two and 215 m for the last one), with a goal of determining the depth of the geologic boundary between the sedimentary and andesitic deposits. The T-2 profile was a short profile (23.5 m), with the objective of calibrating the results with data provided by the paleoseismic trenching previously developed in the area of interest. For these tests, two electrode arrays of types Wenner-Schlumberger and Dipole-Dipole, were used. For the inversion routine, two regularized least-squares methods were used: the smoothness-constrained method and robust inversion. The long electrical resistivity tomography profiles were able to identify a set of electrical anomalies associated with the andesitic basalt and the Tertiary sediments and with that, the contact geometry between these formations. In these profiles, fault angle measurements ranged from 60° to 80° with respect to the ground surface. In the T-2 profile, the electrical anomalies showed a good association with the results of the paleoseismic study. This allowed identification of the colluvium and alluvium covering the gravel and sand debris that mark the gradual transition to the soils of the Caimito Formation. Finally, a set of 2D synthetic models was developed for each of the T-1, T-3, and T-4 profiles with the objective of optimizing interpretation of the field results.

  13. LIM kinase/cofilin dysregulation promotes macrothrombocytopenia in severe von Willebrand disease-type 2B

    Science.gov (United States)

    Poirault-Chassac, Sonia; Adam, Frédéric; Muczynski, Vincent; Aymé, Gabriel; Casari, Caterina; Bordet, Jean-Claude; Soukaseum, Christelle; Rothschild, Chantal; Proulle, Valérie; Pietrzyk-Nivau, Audrey; Berrou, Eliane; Christophe, Olivier D.; Rosa, Jean-Philippe; Lenting, Peter J.; Bryckaert, Marijke; Baruch, Dominique

    2016-01-01

    von Willebrand disease type 2B (VWD-type 2B) is characterized by gain-of-function mutations of von Willebrand factor (vWF) that enhance its binding to platelet glycoprotein Ibα and alter the protein’s multimeric structure. Patients with VWD-type 2B display variable extents of bleeding associated with macrothrombocytopenia and sometimes with thrombopathy. Here, we addressed the molecular mechanism underlying the severe macrothrombocytopenia both in a knockin murine model for VWD-type 2B by introducing the p.V1316M mutation in the murine Vwf gene and in a patient bearing this mutation. We provide evidence of a profound defect in megakaryocyte (MK) function since: (a) the extent of proplatelet formation was drastically decreased in 2B MKs, with thick proplatelet extensions and large swellings; and (b) 2B MKs presented actin disorganization that was controlled by upregulation of the RhoA/LIM kinase (LIMK)/cofilin pathway. In vitro and in vivo inhibition of the LIMK/cofilin signaling pathway rescued actin turnover and restored normal proplatelet formation, platelet count, and platelet size. These data indicate, to our knowledge for the first time, that the severe macrothrombocytopenia in VWD-type 2B p.V1316M is due to an MK dysfunction that originates from a constitutive activation of the RhoA/LIMK/cofilin pathway and actin disorganization. This suggests a potentially new function of vWF during platelet formation that involves regulation of actin dynamics. PMID:27734030

  14. The Hawaiian Freshwater Algal Database (HfwADB): a laboratory LIMS and online biodiversity resource.

    Science.gov (United States)

    Sherwood, Alison R; Wang, Norman; Carlile, Amy L; Neumann, Jessica M; Wolfgruber, Thomas K; Presting, Gernot G

    2012-10-25

    Biodiversity databases serve the important role of highlighting species-level diversity from defined geographical regions. Databases that are specially designed to accommodate the types of data gathered during regional surveys are valuable in allowing full data access and display to researchers not directly involved with the project, while serving as a Laboratory Information Management System (LIMS). The Hawaiian Freshwater Algal Database, or HfwADB, was modified from the Hawaiian Algal Database to showcase non-marine algal specimens collected from the Hawaiian Archipelago by accommodating the additional level of organization required for samples including multiple species. The Hawaiian Freshwater Algal Database is a comprehensive and searchable database containing photographs and micrographs of samples and collection sites, geo-referenced collecting information, taxonomic data and standardized DNA sequence data. All data for individual samples are linked through unique 10-digit accession numbers ("Isolate Accession"), the first five of which correspond to the collection site ("Environmental Accession"). Users can search online for sample information by accession number, various levels of taxonomy, habitat or collection site. HfwADB is hosted at the University of Hawaii, and was made publicly accessible in October 2011. At the present time the database houses data for over 2,825 samples of non-marine algae from 1,786 collection sites from the Hawaiian Archipelago. These samples include cyanobacteria, red and green algae and diatoms, as well as lesser representation from some other algal lineages. HfwADB is a digital repository that acts as a Laboratory Information Management System for Hawaiian non-marine algal data. Users can interact with the repository through the web to view relevant habitat data (including geo-referenced collection locations) and download images of collection sites, specimen photographs and micrographs, and DNA sequences. It is publicly

  15. The Hawaiian Freshwater Algal Database (HfwADB: a laboratory LIMS and online biodiversity resource

    Directory of Open Access Journals (Sweden)

    Sherwood Alison R

    2012-10-01

    Full Text Available Abstract Background Biodiversity databases serve the important role of highlighting species-level diversity from defined geographical regions. Databases that are specially designed to accommodate the types of data gathered during regional surveys are valuable in allowing full data access and display to researchers not directly involved with the project, while serving as a Laboratory Information Management System (LIMS. The Hawaiian Freshwater Algal Database, or HfwADB, was modified from the Hawaiian Algal Database to showcase non-marine algal specimens collected from the Hawaiian Archipelago by accommodating the additional level of organization required for samples including multiple species. Description The Hawaiian Freshwater Algal Database is a comprehensive and searchable database containing photographs and micrographs of samples and collection sites, geo-referenced collecting information, taxonomic data and standardized DNA sequence data. All data for individual samples are linked through unique 10-digit accession numbers (“Isolate Accession”, the first five of which correspond to the collection site (“Environmental Accession”. Users can search online for sample information by accession number, various levels of taxonomy, habitat or collection site. HfwADB is hosted at the University of Hawaii, and was made publicly accessible in October 2011. At the present time the database houses data for over 2,825 samples of non-marine algae from 1,786 collection sites from the Hawaiian Archipelago. These samples include cyanobacteria, red and green algae and diatoms, as well as lesser representation from some other algal lineages. Conclusions HfwADB is a digital repository that acts as a Laboratory Information Management System for Hawaiian non-marine algal data. Users can interact with the repository through the web to view relevant habitat data (including geo-referenced collection locations and download images of collection sites, specimen

  16. FluxCTTX: A LIMS-based tool for management and analysis of cytotoxicity assays data.

    Science.gov (United States)

    Faria-Campos, Alessandra C; Balottin, Luciene B; Zuin, Gianlucca; Garcia, Vinicius; Batista, Paulo H S; Granjeiro, José M; Campos, Sérgio V A

    2015-01-01

    Cytotoxicity assays have been used by researchers to screen for cytotoxicity in compound libraries. Researchers can either look for cytotoxic compounds or screen "hits" from initial high-throughput drug screens for unwanted cytotoxic effects before investing in their development as a pharmaceutical. These assays may be used as an alternative to animal experimentation and are becoming increasingly important in modern laboratories. However, the execution of these assays in large scale and different laboratories requires, among other things, the management of protocols, reagents, cell lines used as well as the data produced, which can be a challenge. The management of all this information is greatly improved by the utilization of computational tools to save time and guarantee quality. However, a tool that performs this task designed specifically for cytotoxicity assays is not yet available. In this work, we have used a workflow based LIMS -- the Flux system -- and the Together Workflow Editor as a framework to develop FluxCTTX, a tool for management of data from cytotoxicity assays performed at different laboratories. The main work is the development of a workflow, which represents all stages of the assay and has been developed and uploaded in Flux. This workflow models the activities of cytotoxicity assays performed as described in the OECD 129 Guidance Document. FluxCTTX presents a solution for the management of the data produced by cytotoxicity assays performed at Interlaboratory comparisons. Its adoption will contribute to guarantee the quality of activities in the process of cytotoxicity tests and enforce the use of Good Laboratory Practices (GLP). Furthermore, the workflow developed is complete and can be adapted to other contexts and different tests for management of other types of data.

  17. Sitios de Cría del Aedes aegypti en la Provincia de Limón, Costa Rica Breeding sites of Aedes aegypti in the province of Limón, Costa Rica

    OpenAIRE

    Rodrigo Marín Rodríguez; Mariela Díaz Ríos; Yaricsa Álvarez Gutiérrez

    2012-01-01

    En Costa Rica el dengue constituye la principal enfermedad de transmisión vectorial. Su control requiere un conocimiento integral de su vector Aedes aegypti, el cual utiliza gran variedad de recipientes artificiales para su cría. Objetivos: Identificar y describir los principales sitios de cría del A. aegypti en la provincia de Limón. Materiales y Métodos: El muestreo se realizó por el personal del programa de control de vectores durante el año 2011; de forma simultáne...

  18. LimTox: a web tool for applied text mining of adverse event and toxicity associations of compounds, drugs and genes.

    Science.gov (United States)

    Cañada, Andres; Capella-Gutierrez, Salvador; Rabal, Obdulia; Oyarzabal, Julen; Valencia, Alfonso; Krallinger, Martin

    2017-05-22

    A considerable effort has been devoted to retrieve systematically information for genes and proteins as well as relationships between them. Despite the importance of chemical compounds and drugs as a central bio-entity in pharmacological and biological research, only a limited number of freely available chemical text-mining/search engine technologies are currently accessible. Here we present LimTox (Literature Mining for Toxicology), a web-based online biomedical search tool with special focus on adverse hepatobiliary reactions. It integrates a range of text mining, named entity recognition and information extraction components. LimTox relies on machine-learning, rule-based, pattern-based and term lookup strategies. This system processes scientific abstracts, a set of full text articles and medical agency assessment reports. Although the main focus of LimTox is on adverse liver events, it enables also basic searches for other organ level toxicity associations (nephrotoxicity, cardiotoxicity, thyrotoxicity and phospholipidosis). This tool supports specialized search queries for: chemical compounds/drugs, genes (with additional emphasis on key enzymes in drug metabolism, namely P450 cytochromes-CYPs) and biochemical liver markers. The LimTox website is free and open to all users and there is no login requirement. LimTox can be accessed at: http://limtox.bioinfo.cnio.es. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  19. Los factores de competitividad de las mipymes agroindustriales del limón mexicano en colima, méxico.

    OpenAIRE

    Magaña Sanchez, Pablo Adrian; Vargas Hernandez, Jose G; Naranjo González, Mario de Jesus; Sandoval Araiza, Felipe de Jesus

    2011-01-01

    El presente trabajo se refiere al estudio de los factores de competitividad de las micro, pequeñas y medianas empresas agroindustriales del limón mexicano en Colima, México, tomando como referencia el modelo del International Institute for Management Development. Se diseñó un nuevo instrumento de recolección de la información consistente en un análisis estadístico, a través del cual se determinaron los factoresy el nivel de competitividad de las empresas ya mencionadas. /Abstract: This paper ...

  20. Sistemas agro y silvopastoriles en El Limón, municipio de Paso de Ovejas, Veracruz, México

    OpenAIRE

    Bautista Tolentino, Marcelo

    2012-01-01

    La caracterización de los sistemas agroforestales es una buena herramienta que en su momento proporciona elementos de análisis para la toma de decisiones en sistemas de uso del suelo. El objetivo de la investigación fue caracterizar los sistemas agroforestales de acuerdo a sus componentes (agrícola, forestal y pecuario) y al principal uso de las especies arbóreas en la comunidad El Limón, municipio de Paso de Ovejas, Veracruz. Además evaluar la producción de biomasa vegetal y composición quím...

  1. Costos de cumplimiento de inocuidad de empacadoras exportadoras de limón "Persa" en Veracruz, México

    OpenAIRE

    Jorge Aguilar Ávila; Alejandro Vaquero Vera; Gustavo Almaguer Vargas; Juan Antonio Leos Rodríguez; Belem Avendaño Ruiz

    2013-01-01

    El objetivo del trabajo fue estimar los costos de cumplimiento de empacadoras de limón Persa de dos regiones de Veracruz al implementar un programa de inocuidad determinar la problemática que enfrentan durante el proceso de certificación en Buenas Prácticas de Manejo (BPM) y los beneficios que perciben al cumplirla. Esta información se recopiló por medio de la aplicación de encuestas a las empacadoras de la región. Las certificadas aprecian la inocuidad como una oportunidad para ganar mercado...

  2. Acción Bactericida del Jugo de Limón Sobre el Staphylococcus Aureus Rosenbach

    Directory of Open Access Journals (Sweden)

    Obregón Botero. Rafael

    1946-04-01

    Full Text Available El objeto de este experimento fue el determinar la influencia que pudiera tener el jugo de limón común (citrus limonia Osbeck sobre el Staphylococcus; aureus Rosenbach. Esta bacteria se encuentra con frecuencia en la piel y el pelo del hombre y de los animales, también en el pus y con frecuencia asociados a muchas otras causantes de abcesos, forúnculos, nacidos, etc. Ocasionalmente pueden causar osteomielitis y endocarditis. En individuos tuberculosos las infecciones causadas por esta bacteria pueden ocasionar la muerte.

  3. Lim homeobox genes in the Ctenophore Mnemiopsis leidyi: the evolution of neural cell type specification.

    Science.gov (United States)

    Simmons, David K; Pang, Kevin; Martindale, Mark Q

    2012-01-13

    Nervous systems are thought to be important to the evolutionary success and diversification of metazoans, yet little is known about the origin of simple nervous systems at the base of the animal tree. Recent data suggest that ctenophores, a group of macroscopic pelagic marine invertebrates, are the most ancient group of animals that possess a definitive nervous system consisting of a distributed nerve net and an apical statocyst. This study reports on details of the evolution of the neural cell type specifying transcription factor family of LIM homeobox containing genes (Lhx), which have highly conserved functions in neural specification in bilaterian animals. Using next generation sequencing, the first draft of the genome of the ctenophore Mnemiopsis leidyi has been generated. The Lhx genes in all animals are represented by seven subfamilies (Lhx1/5, Lhx3/4, Lmx, Islet, Lhx2/9, Lhx6/8, and LMO) of which four were found to be represented in the ctenophore lineage (Lhx1/5, Lhx3/4, Lmx, and Islet). Interestingly, the ctenophore Lhx gene complement is more similar to the sponge complement (sponges do not possess neurons) than to either the cnidarian-bilaterian or placozoan Lhx complements. Using whole mount in situ hybridization, the Lhx gene expression patterns were examined and found to be expressed around the blastopore and in cells that give rise to the apical organ and putative neural sensory cells. This research gives us a first look at neural cell type specification in the ctenophore M. leidyi. Within M. leidyi, Lhx genes are expressed in overlapping domains within proposed neural cellular and sensory cell territories. These data suggest that Lhx genes likely played a conserved role in the patterning of sensory cells in the ancestor of sponges and ctenophores, and may provide a link to the expression of Lhx orthologs in sponge larval photoreceptive cells. Lhx genes were later co-opted into patterning more diversified complements of neural and non-neural cell

  4. Lim homeobox genes in the Ctenophore Mnemiopsis leidyi: the evolution of neural cell type specification

    Directory of Open Access Journals (Sweden)

    Simmons David K

    2012-01-01

    Full Text Available Abstract Background Nervous systems are thought to be important to the evolutionary success and diversification of metazoans, yet little is known about the origin of simple nervous systems at the base of the animal tree. Recent data suggest that ctenophores, a group of macroscopic pelagic marine invertebrates, are the most ancient group of animals that possess a definitive nervous system consisting of a distributed nerve net and an apical statocyst. This study reports on details of the evolution of the neural cell type specifying transcription factor family of LIM homeobox containing genes (Lhx, which have highly conserved functions in neural specification in bilaterian animals. Results Using next generation sequencing, the first draft of the genome of the ctenophore Mnemiopsis leidyi has been generated. The Lhx genes in all animals are represented by seven subfamilies (Lhx1/5, Lhx3/4, Lmx, Islet, Lhx2/9, Lhx6/8, and LMO of which four were found to be represented in the ctenophore lineage (Lhx1/5, Lhx3/4, Lmx, and Islet. Interestingly, the ctenophore Lhx gene complement is more similar to the sponge complement (sponges do not possess neurons than to either the cnidarian-bilaterian or placozoan Lhx complements. Using whole mount in situ hybridization, the Lhx gene expression patterns were examined and found to be expressed around the blastopore and in cells that give rise to the apical organ and putative neural sensory cells. Conclusion This research gives us a first look at neural cell type specification in the ctenophore M. leidyi. Within M. leidyi, Lhx genes are expressed in overlapping domains within proposed neural cellular and sensory cell territories. These data suggest that Lhx genes likely played a conserved role in the patterning of sensory cells in the ancestor of sponges and ctenophores, and may provide a link to the expression of Lhx orthologs in sponge larval photoreceptive cells. Lhx genes were later co-opted into patterning more

  5. The Province and Port of Limón: Metaphors for Afro-Costa Rican Black Identity

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    Carmen Hutchinson Miller Ph.D

    2013-06-01

    Full Text Available A significant number of Afro-Caribbean immigrant workers from the Anglo Spanish, and French Caribbean migrated to the Central American country called Costa Rica, to work on the construction of a railroad, 140 years ago (1872. Strained economic conditions in their homelands in the late 19th century was the push factor that forced them out of their homes in search for better opportunities that would improve their own and the lives of the families they left behind. Large numbers of these immigrants were forced to settle in the province and port of Limón. The unintentional settlement resulted in the biological reproduction of the present Afro-Costa Rican population, who are still not fully accepted within the society. This group of people maintains cultural aspects of the Caribbean immigrant culture, particularly that of Jamaica. This paper will demonstrate from the perspective of history some of the reasons why the province and port of Limón can be considered metaphors for Afro-Costa Rican Black Identity, using both primary sources and secondary information found in archival documents, newspaper clippings, interviews, and other publications.

  6. Voces pancaribeñas en América Central: Anacristina Rossi y Limón Reggae

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    Carol Pearson

    2013-08-01

    Full Text Available En Limón Reggae, la autora describe las experiencias de una protagonista que no tiene entrada en ningún grupo: nación, raza o grupo político. Son representaciones de las comunidades afrocaribeñas junto con la lucha revolucionaria en El Salvador y las experiencias de los niños de la calle. En After Exile, Amy Kaminsky plantea la presencia de imágenes corporales en la obra de muchos escritores latinoamericanos exiliados o expatriados. Rossi explora situaciones desesperadas centroamericanas y mundiales, junto a una lucha de resistencia. In Limón Reggae, the author describes the experiences of a protagonist who never finds acceptance or inclusion in any group, whether that of nation, ethnicity or politics. We find representations of Afro-Caribbean communities along with the revolutionary struggle in El Salvador and the experiences of present-day street children. In After Exile, Amy Kaminsky acknowledges the presence of images of the body in the work of many Latin American writers who have been exiled or have lived expatriate lives. Rossi explores desperate situations, both Central American and worldwide, together with a resistance struggle.

  7. 棉花LIM蛋白基因克隆及表达分析%Cloning and Expression Analysis of a LIM-Domain Protein Gene from Cotton (Gossypium hirsuturm L. )

    Institute of Scientific and Technical Information of China (English)

    Ming LUO; Yue-hua XIAO; Zheng-sheng ZHANG; Wei-gou FANG; Lei HOU; Xiao-ying LUO; Shang-yong ZHENG; Yan PEI

    2002-01-01

    @@ LIM-domain proteins are implicated in multiple cellular and developmental processes in eukaryotes. Their essential roles have been well characterized in animals. It was revealed that LIM-domain protein plays an important role in various cellular processes, including construction of cytoskeleton, transcription control and signal transduction.

  8. Sitios de Cría del Aedes aegypti en la Provincia de Limón, Costa Rica Breeding sites of Aedes aegypti in the province of Limón, Costa Rica

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    Rodrigo Marín Rodríguez

    2012-06-01

    Full Text Available En Costa Rica el dengue constituye la principal enfermedad de transmisión vectorial. Su control requiere un conocimiento integral de su vector Aedes aegypti, el cual utiliza gran variedad de recipientes artificiales para su cría. Objetivos: Identificar y describir los principales sitios de cría del A. aegypti en la provincia de Limón. Materiales y Métodos: El muestreo se realizó por el personal del programa de control de vectores durante el año 2011; de forma simultánea en los seis cantones que conforman la provincia de Limón. Resultados: Se identifico la presencia de A. aegypti en los seis cantones de la provincia de Limón. Los sitios de cría mas frecuentes fueron las llantas, los tarros o depósitos inservibles, los baldes y los tanques. Conclusiones: La proliferación del A. aegypti está directamente relacionado con el hombre, sus malos hábitos, y por la calidad de los servicios de salud de protección y mejoramiento del ambiente humano. Estos resultados demuestran que el abordaje del control del A. aegypti debe ser integral.Dengue is the main disease due to vectorial transmission in Costa Rica. Its control requires comprehensive knowledge of its vector which uses a wide variety of artificial containers for breeding. Objectives: To identify and describe Aedes aegypti breeding sites present in the Limon province of Costa Rica. Materials and methods: this survey was carried out during the year 2011 by personnel of the Vector Control Program working simultaneously in six municipalities of the studied region. Results: We identified the presence of Aedes aegypti in six municipalities in the province of Limon. The most frequent breeding sites were tires, cans or unusable deposits, buckets and tanks. Conclusions: Proliferation of Aedes aegypti is directly related to man, his bad habits, and to the quality of health care models regarding protection and improvement of the human environment. These results show that the approach to

  9. Migraciones entre países del sur: Los cambios y las continuidades en los flujos limítrofes hacia Argentina

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    Eduardo León Bologna

    2010-01-01

    Este artículo observa las migraciones limítrofes hacia Argentina considerando que los cambios suceden bajo los efectos conjuntos de las coyunturas externas y de un proceso acumulativo que tiene sus propios tiempos, y que juntos determinan su continuidad o extinción.

  10. Solution structure of the LIM-homeodomain transcription factor complex Lhx3/Ldb1 and the effects of a pituitary mutation on key Lhx3 interactions.

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    Mugdha Bhati

    Full Text Available Lhx3 is a LIM-homeodomain (LIM-HD transcription factor that regulates neural cell subtype specification and pituitary development in vertebrates, and mutations in this protein cause combined pituitary hormone deficiency syndrome (CPHDS. The recently published structures of Lhx3 in complex with each of two key protein partners, Isl1 and Ldb1, provide an opportunity to understand the effect of mutations and posttranslational modifications on key protein-protein interactions. Here, we use small-angle X-ray scattering of an Ldb1-Lhx3 complex to confirm that in solution the protein is well represented by our previously determined NMR structure as an ensemble of conformers each comprising two well-defined halves (each made up of LIM domain from Lhx3 and the corresponding binding motif in Ldb1 with some flexibility between the two halves. NMR analysis of an Lhx3 mutant that causes CPHDS, Lhx3(Y114C, shows that the mutation does not alter the zinc-ligation properties of Lhx3, but appears to cause a structural rearrangement of the hydrophobic core of the LIM2 domain of Lhx3 that destabilises the domain and/or reduces the affinity of Lhx3 for both Ldb1 and Isl1. Thus the mutation would affect the formation of Lhx3-containing transcription factor complexes, particularly in the pituitary gland where these complexes are required for the production of multiple pituitary cell types and hormones.

  11. Comparative study of sea ice dynamics simulations with a Maxwell elasto-brittle rheology and the elastic-viscous-plastic rheology in NEMO-LIM3

    Science.gov (United States)

    Raulier, Jonathan; Dansereau, Véronique; Fichefet, Thierry; Legat, Vincent; Weiss, Jérôme

    2017-04-01

    Sea ice is a highly dynamical environment characterized by a dense mesh of fractures or leads, constantly opening and closing over short time scales. This characteristic geomorphology is linked to the existence of linear kinematic features, which consist of quasi-linear patterns emerging from the observed strain rate field of sea ice. Standard rheologies used in most state-of-the-art sea ice models, like the well-known elastic-viscous-plastic rheology, are thought to misrepresent those linear kinematic features and the observed statistical distribution of deformation rates. Dedicated rheologies built to catch the processes known to be at the origin of the formation of leads are developed but still need evaluations on the global scale. One of them, based on a Maxwell elasto-brittle formulation, is being integrated in the NEMO-LIM3 global ocean-sea ice model (www.nemo-ocean.eu; www.elic.ucl.ac.be/lim). In the present study, we compare the results of the sea ice model LIM3 obtained with two different rheologies: the elastic-viscous-plastic rheology commonly used in LIM3 and a Maxwell elasto-brittle rheology. This comparison is focused on the statistical characteristics of the simulated deformation rate and on the ability of the model to reproduce the existence of leads within the ice pack. The impact of the lead representation on fluxes between ice, atmosphere and ocean is also assessed.

  12. Managing Requirement Volatility in an Ontology-Driven Clinical LIMS Using Category Theory. International Journal of Telemedicine and Applications

    CERN Document Server

    Shaban-Nejad, Arash; Kassab, Mohamad; Haarslev, Volker; 10.1155/2009/917826

    2009-01-01

    Requirement volatility is an issue in software engineering in general, and in Web-based clinical applications in particular, which often originates from an incomplete knowledge of the domain of interest. With advances in the health science, many features and functionalities need to be added to, or removed from, existing software applications in the biomedical domain. At the same time, the increasing complexity of biomedical systems makes them more difficult to understand, and consequently it is more difficult to define their requirements, which contributes considerably to their volatility. In this paper, we present a novel agent-based approach for analyzing and managing volatile and dynamic requirements in an ontology-driven laboratory information management system (LIMS) designed for Web-based case reporting in medical mycology. The proposed framework is empowered with ontologies and formalized using category theory to provide a deep and common understanding of the functional and nonfunctional requirement hi...

  13. [Diet of the capybara Hydrochaeris hydrochaeris (Rodentia: Hydrocharidae) in Caño Limón, Arauca, Colombia].

    Science.gov (United States)

    Forero-Montaña, Jimena; Betancur, Julio; Cavelier, Jaime

    2003-06-01

    We studied the composition and seasonal variation of the diet of the capybara (Hydrochaeris hydrochaeris) in the flooded savannas of Caño Limón, Colombia. This was achieved by direct observation of the consumption patterns of these animals. The capybaras only consumed plants, and their diet included 89 species of 22 families. Sixty three percent of these plant species had not been reported before. The most commonly consumed plants (94% of the diet), belonged to the Poaceae, Cyperaceae, Leguminosae and Pontederiaceae. Only seven species represented 60% of the total diet: the grasses Hymenachne amplexicaulis (16.9%), Digitaria bicornis (4.5%) and Panicum maximum (4.4%) and the Cyperaceae Rynchospora corymbosa (4.4%). There was seasonal variation in the diet composition of capybaras.

  14. L’avenir des sabliers. Sur des poèmes de Sélim Baghli et de Farid Laroussi

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    Pierre Popovic

    2013-11-01

    Full Text Available Autant la poésie de Sélim Baghli peut être hachée, hésitante, inquiète d’elle-même, autant celle de Farid Laroussi frappe d’estoc, est assurée et ferme. Quand les vers de Baghli cooptent Verlaine, la prose de Laroussi affiche plus d’une accointance avec celle de Rimbaud. Cet article ne se propose pas de produire un brillant paradoxe à la manière des critiques littéraires vaporeux, mais d’essayer de comprendre comment des poèmes de Baghli et de Laroussi, en mettant à leur manière en scène un sujet qui résiste aux ravages du passé tout en étant dans le temps de l’après-coup, se trouvent plus ou moins travaillés par le thème de « l’Algérie malgré tout ». As much as Sélim Baghli’s poetry can be choppy, faltering, and self-conscious, Farid Laroussi’s is direct, self-assured and firm. Where Baghli’s verses co-opt Verlaine, Laroussi’s prose has a clear link to Rimbaud. This article does not attempt to illuminate an obvious paradox in the hazy style of literary critique, but instead it attempts to understand how the poems of Baghli and Laroussi portray, in their own way, a subject that resists the ravages of the past all the while existing in the post-coup era, finding themselves more or less shaped by the theme of “Algeria in spite of it all.”

  15. Maximizing neonatal early onset group B streptococcal disease prevention with universal culture screening at 35 to 37 weeks gestation: a comparison of GBS detection rates between LIM broth and CNA culture media.

    Science.gov (United States)

    Orsello, Christopher; Dommermuth, Ronald

    2003-06-01

    Group B streptococcal (GBS) disease is the most common cause of early onset neonatal sepsis. The Centers for Disease Control (CDC) recommends performing recto-vaginal cultures on pregnant woman to detect GBS, followed by treatment of women with positive cultures. Our facility adopted selective culture screening in 1997 using a colistin-naladixic acid (CNA) plate media instead of the more expensive LIM broth media. CNA plate cultures cost one third that of LIM broth and allow for final results in 24 hours, versus 48-72 hours with LIM broth. We hypothesized that CNA media saves time, money, and detects GBS as effectively as LIM broth. This study determined which media is superior at detecting recto-vaginal GBS. This was a case-control study involving 152 consecutive pregnant patients at 35-37 weeks from August 1 to October 1, 2001, at Naval Hospital Bremerton, Wash. We obtained two recto-vaginal swabs from each patient. One was cultured in LIM broth and the other on CNA medium. We then compared differences in the rates of positive cultures with LIM broth versus CNA medium using chi-square statistics and calculation of odds ratios (OR). LIM broth detected GBS in 35 of 145 (24.19%) women versus 21 of 145 (14.5%) using CNA. CNA failed to detect GBS in 15 cases in which LIM broth succeeded (OR=1.88; 95% CI=1.03-3.4). LIM broth is superior at detecting maternal GBS colonization and is recommended over CNA plate to maximize prevention of early onset neonatal GBS disease.

  16. The terminal basal mitosis of chicken retinal Lim1 horizontal cells is not sensitive to cisplatin-induced cell cycle arrest.

    Science.gov (United States)

    Shirazi Fard, Shahrzad; Thyselius, Malin; All-Ericsson, Charlotta; Hallböök, Finn

    2014-01-01

    For proper development, cells need to coordinate proliferation and cell cycle-exit. This is mediated by a cascade of proteins making sure that each phase of the cell cycle is controlled before the initiation of the next. Retinal progenitor cells divide during the process of interkinetic nuclear migration, where they undergo S-phase on the basal side, followed by mitoses on the apical side of the neuroepithelium. The final cell cycle of chicken retinal horizontal cells (HCs) is an exception to this general cell cycle behavior. Lim1 expressing (+) horizontal progenitor cells (HPCs) have a heterogenic final cell cycle, with some cells undergoing a terminal mitosis on the basal side of the retina. The results in this study show that this terminal basal mitosis of Lim1+ HPCs is not dependent on Chk1/2 for its regulation compared to retinal cells undergoing interkinetic nuclear migration. Neither activating nor blocking Chk1 had an effect on the basal mitosis of Lim1+ HPCs. Furthermore, the Lim1+ HPCs were not sensitive to cisplatin-induced DNA damage and were able to continue into mitosis in the presence of γ-H2AX without activation of caspase-3. However, Nutlin3a-induced expression of p21 did reduce the mitoses, suggesting the presence of a functional p53/p21 response in HPCs. In contrast, the apical mitoses were blocked upon activation of either Chk1/2 or p21, indicating the importance of these proteins during the process of interkinetic nuclear migration. Inhibiting Cdk1 blocked M-phase transition both for apical and basal mitoses. This confirmed that the cyclin B1-Cdk1 complex was active and functional during the basal mitosis of Lim1+ HPCs. The regulation of the final cell cycle of Lim1+ HPCs is of particular interest since it has been shown that the HCs are able to sustain persistent DNA damage, remain in the cell cycle for an extended period of time and, consequently, survive for months.

  17. A Missense Mutation in the LIM2 Gene Is Associated with Autosomal Recessive Presenile Cataract in an Inbred Iraqi Jewish Family

    Science.gov (United States)

    Pras, Eran; Levy-Nissenbaum, Etgar; Bakhan, Tangiz; Lahat, Hadas; Assia, Ehud; Geffen-Carmi, Noa; Frydman, Moshe; Goldman, Boleslaw; Pras, Elon

    2002-01-01

    In an inbred Iraqi Jewish family, we have studied three siblings with presenile cataract first noticed between the ages of 20 and 51 years and segregating in an autosomal recessive mode. Using microsatellite repeat markers in close proximity to 25 genes and loci previously associated with congenital cataracts in humans and mice, we identified five markers on chromosome 19q that cosegregated with the disease. Sequencing of LIM2, one of two candidate genes in this region, revealed a homozygous T→G change resulting in a phenylalanine-to-valine substitution at position 105 of the protein. To our knowledge, this constitutes the first report, in humans, of cataract formation associated with a mutation in LIM2. Studies of late-onset single-gene cataracts may provide insight into the pathogenesis of the more common age-related cataracts. PMID:11917274

  18. Automated washing of FTA Card punches and PCR setup for reference samples using a LIMS-controlled Sias Xantus automated liquid handler

    DEFF Research Database (Denmark)

    2009-01-01

    We have implemented and validated automated methods for washing FTA Card punches containing buccal samples and subsequent PCR setup using a Sias Xantus automated liquid handler. The automated methods were controlled by worklists generated by our LabWare Laboratory Information Management System (L...... (LIMS). The protocols were validated according to EN/ISO 17025 for use with STR amplifications kits AmpFlSTR« Identifiler« and Y-filer« (Applied Biosystems)...

  19. The pH sensibility of actin-bundling LIM proteins is governed by the acidic properties of their C-terminal domain.

    Science.gov (United States)

    Moes, Danièle; Hoffmann, Céline; Dieterle, Monika; Moreau, Flora; Neumann, Katrin; Papuga, Jessica; Furtado, Angela Tavares; Steinmetz, André; Thomas, Clément

    2015-08-19

    Actin-bundling Arabidopsis LIM proteins are subdivided into two subfamilies differing in their pH sensitivity. Widely-expressed WLIMs are active under low and high physiologically-relevant pH conditions, whereas pollen-enriched PLIMs are inactivated by pH values above 6.8. By a domain swapping approach we identified the C-terminal (Ct) domain of PLIMs as the domain responsible for pH responsiveness. Remarkably, this domain conferred pH sensitivity to LIM proteins, when provided "in trans" (i.e., as a single, independent, peptide), indicating that it operates through the interaction with another domain. An acidic 6xc-Myc peptide functionally mimicked the Ct domain of PLIMs and efficiently inhibited LIM actin bundling activity under high pH conditions. Together, our data suggest a model where PLIMs are regulated by an intermolecular interaction between their acidic Ct domain and another, yet unidentified, domain. Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  20. Nuevo equipo de proceso de destilación fraccionada en continuo por arrastre con vapor de aceites esenciales del jugo de limón mexicano

    Directory of Open Access Journals (Sweden)

    Francisco J. Padilla M.

    2007-01-01

    Full Text Available En este trabajo se presenta un nuevo equipo llamado Destilador-Fraccionador Horizontal, para realizar el procesos de destilación fraccionada en continuo por arrastre de vapor de aceites esenciales obtenidos a partir del jugo de limón mexicano (Citrus aurantifolia. La capacidad de fraccionación del nuevo equipo es analizada con soluciones modelo y soluciones reales de jugo de limón. Se emplean diseños experimentales y métodos estadísticos para investigar el desempeño del equipo y para validar su capacidad de fraccionar. Mediante la determinación de la relación S/F (gramos de vapor necesario para extraer 1 g de aceite destilado en la fase vapor, se determina que el nuevo equipo requiere una menor cantidad de energía para lograr la separación de los aceites esenciales. Eficiencias de extracción de aceites esenciales superiores al 90% son alcanzadas empleando las soluciones reales de jugo de limón.

  1. The LIM homeodomain protein Lhx6 regulates maturation of interneurons and network excitability in the mammalian cortex.

    Science.gov (United States)

    Neves, Guilherme; Shah, Mala M; Liodis, Petros; Achimastou, Angeliki; Denaxa, Myrto; Roalfe, Grant; Sesay, Abdul; Walker, Matthew C; Pachnis, Vassilis

    2013-08-01

    Deletion of LIM homeodomain transcription factor-encoding Lhx6 gene in mice results in defective tangential migration of cortical interneurons and failure of differentiation of the somatostatin (Sst)- and parvalbumin (Pva)-expressing subtypes. Here, we characterize a novel hypomorphic allele of Lhx6 and demonstrate that reduced activity of this locus leads to widespread differentiation defects in Sst(+) interneurons, but relatively minor and localized changes in Pva(+) interneurons. The reduction in the number of Sst-expressing cells was not associated with a loss of interneurons, because the migration and number of Lhx6-expressing interneurons and expression of characteristic molecular markers, such as calretinin or Neuropeptide Y, were not affected in Lhx6 hypomorphic mice. Consistent with a selective deficit in the differentiation of Sst(+) interneurons in the CA1 subfield of the hippocampus, we observed reduced expression of metabotropic Glutamate Receptor 1 in the stratum oriens and characteristic changes in dendritic inhibition, but normal inhibitory input onto the somatic compartment of CA1 pyramidal cells. Moreover, Lhx6 hypomorphs show behavioral, histological, and electroencephalographic signs of recurrent seizure activity, starting from early adulthood. These results demonstrate that Lhx6 plays an important role in the maturation of cortical interneurons and the formation of inhibitory circuits in the mammalian cortex.

  2. Rho-kinase regulates tissue morphogenesis via non-muscle myosin and LIM-kinase during Drosophila development

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    Settleman Jeffrey

    2006-08-01

    Full Text Available Abstract Background The Rho-kinases (ROCKs are major effector targets of the activated Rho GTPase that have been implicated in many of the Rho-mediated effects on cell shape and movement via their ability to affect acto-myosin contractility. The role of ROCKs in cell shape change and motility suggests a potentially important role for Rho-ROCK signaling in tissue morphogenesis during development. Indeed, in Drosophila, a single ROCK ortholog, DRok, has been identified and has been found to be required for establishing planar cell polarity. Results We have examined a potential role for DRok in additional aspects of tissue morphogenesis using an activated form of the protein in transgenic flies. Our findings demonstrate that DRok activity can influence multiple morphogenetic processes, including eye and wing development. Furthermore, genetic studies reveal that Drok interacts with multiple downstream effectors of the Rho GTPase signaling pathway, including non-muscle myosin heavy chain, adducin, and Diaphanous in those developmental processes. Finally, in overexpression studies, we determined that Drok and Drosophila Lim-kinase interact in the developing nervous system. Conclusion These findings indicate widespread diverse roles for DRok in tissue morphogenesis during Drosophila development, in which multiple DRok substrates appear to be required.

  3. Expression and function of the LIM-homeodomain transcription factor Islet-1 in the developing and mature vertebrate retina.

    Science.gov (United States)

    Bejarano-Escobar, Ruth; Álvarez-Hernán, Guadalupe; Morona, Ruth; González, Agustín; Martín-Partido, Gervasio; Francisco-Morcillo, Javier

    2015-09-01

    The LIM-homeodomain transcription factor Islet-1 (Isl1) has been widely used as a marker of different subtypes of neurons in the developing and mature retina of vertebrates. During retinal neurogenesis, early Isl1 expression is detected in the nuclei of neuroblasts that give rise to ganglion, amacrine, bipolar, and horizontal cells. In the mature retina, Isl1 expression is restricted to the nuclei of ganglion cells, cholinergic amacrine cells, ON-bipolar cells, and subpopulations of horizontal cells. Recent studies have explored the functional mechanisms of Isl1 during specification and differentiation of these retinal cell types. Thus, conditional inactivation of Isl1 in the developing mouse retina disrupts retinal function, and also results in optic nerve hypoplasia, marked reductions in mature ganglion, amacrine, and bipolar cells, and a substantial increase in horizontal cells. Furthermore, conditional knockout shows delayed ganglion cell axon growth, ganglion cell axon guidance error, and ganglion cell nerve fiber defasciculation. These data together suggest a possible role for Isl1 in the early differentiation and maintenance of different vertebrate retinal cell types. This review examines whether the expression pattern of Isl1 during vertebrate retinal development is conserved across vertebrate species, and discusses current understanding of the developmental functions of Isl1 in retinogenesis.

  4. Interactions between Arctic sea ice drift, concentration and thickness modeled by NEMO-LIM3 at different resolutions

    Science.gov (United States)

    Docquier, David; Massonnet, François; Raulier, Jonathan; Lecomte, Olivier; Fichefet, Thierry

    2016-04-01

    Sea ice concentration and thickness have substantially decreased in the Arctic since the beginning of the satellite era. As a result, mechanical strength has decreased allowing more fracturing and leading to increased sea ice drift. However, recent studies have highlighted that the interplay between sea ice thermodynamics and dynamics is poorly represented in contemporary global climate model (GCM) simulations. Thus, the considerable inter-model spread in terms of future sea ice extent projections could be reduced by better understanding the interactions between drift, concentration and thickness. This study focuses on the results coming from the global coupled ocean-sea ice model NEMO-LIM3 between 1979 and 2012. Three different simulations are forced by the Drakkar Forcing Set (DFS) 5.2 and run on the global tripolar ORCA grid at spatial resolutions of 0.25, 1° and 2°. The relation between modeled sea ice drift, concentration and thickness is further analyzed, compared to observations and discussed in the framework of the above-mentioned poor representation. It is proposed as a process-based metric for evaluating model performance. This study forms part of the EU Horizon 2020 PRIMAVERA project aiming at developing a new generation of advanced and well-evaluated high-resolution GCMs.

  5. Elevated LIM kinase 1 in nonmetastatic prostate cancer reflects its role in facilitating androgen receptor nuclear translocation.

    Science.gov (United States)

    Mardilovich, Katerina; Gabrielsen, Mads; McGarry, Lynn; Orange, Clare; Patel, Rachana; Shanks, Emma; Edwards, Joanne; Olson, Michael F

    2015-01-01

    Prostate cancer affects a large proportion of the male population, and is primarily driven by androgen receptor (AR) activity. First-line treatment typically consists of reducing AR signaling by hormone depletion, but resistance inevitably develops over time. One way to overcome this issue is to block AR function via alternative means, preferably by inhibiting protein targets that are more active in tumors than in normal tissue. By staining prostate cancer tumor sections, elevated LIM kinase 1 (LIMK1) expression and increased phosphorylation of its substrate Cofilin were found to be associated with poor outcome and reduced survival in patients with nonmetastatic prostate cancer. A LIMK-selective small molecule inhibitor (LIMKi) was used to determine whether targeted LIMK inhibition was a potential prostate cancer therapy. LIMKi reduced prostate cancer cell motility, as well as inhibiting proliferation and increasing apoptosis in androgen-dependent prostate cancer cells more effectively than in androgen-independent prostate cancer cells. LIMK inhibition blocked ligand-induced AR nuclear translocation, reduced AR protein stability and transcriptional activity, consistent with its effects on proliferation and survival acting via inhibition of AR activity. Furthermore, inhibition of LIMK activity increased αTubulin acetylation and decreased AR interactions with αTubulin, indicating that the role of LIMK in regulating microtubule dynamics contributes to AR function. These results indicate that LIMK inhibitors could be beneficial for the treatment of prostate cancer both by reducing nuclear AR translocation, leading to reduced proliferation and survival, and by inhibiting prostate cancer cell dissemination.

  6. Transcriptional control of axonal guidance and sorting in dorsal interneurons by the Lim-HD proteins Lhx9 and Lhx1

    Directory of Open Access Journals (Sweden)

    Schejter Adi

    2009-06-01

    Full Text Available Abstract Background Lim-HD proteins control crucial aspects of neuronal differentiation, including subtype identity and axonal guidance. The Lim-HD proteins Lhx2/9 and Lhx1/5 are expressed in the dorsal spinal interneuron populations dI1 and dI2, respectively. While they are not required for cell fate acquisition, their role in patterning the axonal trajectory of dI1 and dI2 neurons remains incompletely understood. Results Using newly identified dI1- and dI2-specific enhancers to trace axonal trajectories originating from these interneurons, we found that each population is subdivided into several distinct groups according to their axonal pathways. dI1 neurons project axons rostrally, either ipsi- or contra-laterally, while dI2 are mostly commissural neurons that project their axons rostrally and caudally. The longitudinal axonal tracks of each neuronal population self-fasciculate to form dI1- and dI2-specific bundles. The dI1 bundles are spatially located ventral relative to dI2 bundles. To examine the functional contribution of Lim-HD proteins to establishment of dI axonal projections, the Lim-HD code of dI neurons was altered by cell-specific ectopic expression. Expression of Lhx1 in dI1 neurons caused a repression of Lhx2/9 and imposed caudal projection to the caudal commissural dI1 neurons. Complementarily, when expressed in dI2 neurons, Lhx9 repressed Lhx1/5 and triggered a bias toward rostral projection in otherwise caudally projecting dI2 neurons, and ventral shift of the longitudinal axonal fascicule. Conclusion The Lim-HD proteins Lhx9 and Lhx1 serve as a binary switch in controlling the rostral versus caudal longitudinal turning of the caudal commissural axons. Lhx1 determines caudal turning and Lhx9 triggers rostral turning.

  7. LIM kinase1 modulates function of membrane type matrix metalloproteinase 1: implication in invasion of prostate cancer cells

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    Chakrabarti Ratna

    2011-01-01

    Full Text Available Abstract Background LIM kinase 1 (LIMK1 is an actin and microtubule cytoskeleton modulatory protein that is overexpressed in a number of cancerous tissues and cells and also promotes invasion and metastasis of prostate and breast cancer cells. Membrane type matrix metalloproteinase 1 (MT1-MMP is a critical modulator of extracellular matrix (ECM turnover through pericellular proteolysis and thus plays crucial roles in neoplastic cell invasion and metastasis. MT1-MMP and its substrates pro-MMP-2 and pro-MMP-9 are often overexpressed in a variety of cancers including prostate cancer and the expression levels correlate with the grade of malignancy in prostate cancer cells. The purpose of this study is to determine any functional relation between LIMK1 and MT1-MMP and its implication in cell invasion. Results Our results showed that treatment with the hydroxamate inhibitor of MT1-MMP, MMP-2 and MMP-9 ilomastat inhibited LIMK1-induced invasion of benign prostate epithelial cells. Over expression of LIMK1 resulted in increased collagenolytic activity of MMP-2, and secretion of pro-MMP2 and pro-MMP-9. Cells over expressing LIMK1 also exhibited increased expression of MT1-MMP, transcriptional activation and its localization to the plasma membrane. LIMK1 physically associates with MT1-MMP and is colocalized with it to the Golgi vesicles. We also noted increased expression of both MT1-MMP and LIMK1 in prostate tumor tissues. Conclusion Our results provide new information on regulation of MT1-MMP function by LIMK1 and showed for the first time, involvement of MMPs in LIMK1 induced cell invasion.

  8. Targeting nitrative stress for attenuating cisplatin-induced downregulation of cochlear LIM domain only 4 and ototoxicity

    Directory of Open Access Journals (Sweden)

    Samson Jamesdaniel

    2016-12-01

    Full Text Available Cisplatin-induced ototoxicity remains a primary dose-limiting adverse effect of this highly effective anticancer drug. The clinical utility of cisplatin could be enhanced if the signaling pathways that regulate the toxic side-effects are delineated. In previous studies, we reported cisplatin-induced nitration of cochlear proteins and provided the first evidence for nitration and downregulation of cochlear LIM domain only 4 (LMO4 in cisplatin ototoxicity. Here, we extend these findings to define the critical role of nitrative stress in cisplatin-induced downregulation of LMO4 and its consequent ototoxic effects in UBOC1 cell cultures derived from sensory epithelial cells of the inner ear and in CBA/J mice. Cisplatin treatment increased the levels of nitrotyrosine and active caspase 3 in UBOC1 cells, which was detected by immunocytochemical and flow cytometry analysis, respectively. The cisplatin-induced nitrative stress and apoptosis were attenuated by co-treatment with SRI110, a peroxynitrite decomposition catalyst (PNDC, which also attenuated the cisplatin-induced downregulation of LMO4 in a dose-dependent manner. Furthermore, transient overexpression of LMO4 in UBOC1 cells prevented cisplatin-induced cytotoxicity while repression of LMO4 exacerbated cisplatin-induced cell death, indicating a direct link between LMO4 protein levels and cisplatin ototoxicity. Finally, auditory brainstem responses (ABR recorded from CBA/J mice indicated that co-treatment with SRI110 mitigated cisplatin-induced hearing loss. Together, these results suggest that cisplatin-induced nitrative stress leads to a decrease in the levels of LMO4, downregulation of LMO4 is a critical determinant in cisplatin-induced ototoxicity, and targeting peroxynitrite could be a promising strategy for mitigating cisplatin-induced hearing loss.

  9. Targeting nitrative stress for attenuating cisplatin-induced downregulation of cochlear LIM domain only 4 and ototoxicity.

    Science.gov (United States)

    Jamesdaniel, Samson; Rathinam, Rajamani; Neumann, William L

    2016-12-01

    Cisplatin-induced ototoxicity remains a primary dose-limiting adverse effect of this highly effective anticancer drug. The clinical utility of cisplatin could be enhanced if the signaling pathways that regulate the toxic side-effects are delineated. In previous studies, we reported cisplatin-induced nitration of cochlear proteins and provided the first evidence for nitration and downregulation of cochlear LIM domain only 4 (LMO4) in cisplatin ototoxicity. Here, we extend these findings to define the critical role of nitrative stress in cisplatin-induced downregulation of LMO4 and its consequent ototoxic effects in UBOC1 cell cultures derived from sensory epithelial cells of the inner ear and in CBA/J mice. Cisplatin treatment increased the levels of nitrotyrosine and active caspase 3 in UBOC1 cells, which was detected by immunocytochemical and flow cytometry analysis, respectively. The cisplatin-induced nitrative stress and apoptosis were attenuated by co-treatment with SRI110, a peroxynitrite decomposition catalyst (PNDC), which also attenuated the cisplatin-induced downregulation of LMO4 in a dose-dependent manner. Furthermore, transient overexpression of LMO4 in UBOC1 cells prevented cisplatin-induced cytotoxicity while repression of LMO4 exacerbated cisplatin-induced cell death, indicating a direct link between LMO4 protein levels and cisplatin ototoxicity. Finally, auditory brainstem responses (ABR) recorded from CBA/J mice indicated that co-treatment with SRI110 mitigated cisplatin-induced hearing loss. Together, these results suggest that cisplatin-induced nitrative stress leads to a decrease in the levels of LMO4, downregulation of LMO4 is a critical determinant in cisplatin-induced ototoxicity, and targeting peroxynitrite could be a promising strategy for mitigating cisplatin-induced hearing loss. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  10. The LIM-only protein FHL2 mediates ras-induced transformation through cyclin D1 and p53 pathways.

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    Charlotte Labalette

    Full Text Available BACKGROUND: Four and a half LIM-only protein 2 (FHL2 has been implicated in multiple signaling pathways that regulate cell growth and tissue homeostasis. We reported previously that FHL2 regulates cyclin D1 expression and that immortalized FHL2-null mouse embryo fibroblasts (MEFs display reduced levels of cyclin D1 and low proliferative activity. METHODOLOGY/PRINCIPAL FINDINGS: Here we address the contribution of FHL2 in cell transformation by investigating the effects of oncogenic Ras in FHL2-null context. We show that H-RasV12 provokes cell cycle arrest accompanied by accumulation of p53 and p16(INK4a in immortalized FHL2(-/- MEFs. These features contrast sharply with Ras transforming activity in wild type cell lines. We further show that establishment of FHL2-null cell lines differs from conventional immortalization scheme by retaining functional p19(ARF/p53 checkpoint that is required for cell cycle arrest imposed by Ras. However, after serial passages of Ras-expressing FHL2(-/- cells, dramatic increase in the levels of D-type cyclins and Rb phosphorylation correlates with the onset of cell proliferation and transformation without disrupting the p19(ARF/p53 pathway. Interestingly, primary FHL2-null cells overexpressing cyclin D1 undergo a classical immortalization process leading to loss of the p19(ARF/p53 checkpoint and susceptibility to Ras transformation. CONCLUSIONS/SIGNIFICANCE: Our findings uncover a novel aspect of cellular responses to mitogenic stimulation and illustrate a critical role of FHL2 in the signalling network that implicates Ras, cyclin D1 and p53.

  11. Effects of different agricultural systems on soil quality in Northern Limón province, Costa Rica.

    Science.gov (United States)

    Cornwell, Emma

    2014-09-01

    Conversion of native rainforest ecosystems in Limón Province of Costa Rica to banana and pineapple monoculture has led to reductions in biodiversity and soil quality. Agroforestry management of cacao (Theobroma cacao) is an alternative system that may maintain the agricultural livelihood of the region while more closely mimicking native ecosystems. This study compared physical, biological and chemical soil quality indicators of a cacao plantation under organic agroforestry management with banana, pineapple, and pasture systems; a native forest nearby served as a control. For bulk density and earthworm analysis, 18 samples were collected between March and April 2012 from each ecosystem paired with 18 samples from the cacao. Cacao had a lower bulk density than banana and pineapple monocultures, but greater than the forest (p agroecosystem paired with three samples from the cacao plantation. Forest and pineapple ecosystems had the lowest pH, cation exchange capacity, and exchangeable nutrient cations, while cacao had the greatest (p < 0.05). Total nutrient levels of P and N were slightly greater in banana, pineapple and pasture than in cacao; probably related to addition of chemical fertilizer and manure from cattle grazing. Forest and cacao also had greater %C, than other ecosystems, which is directly related to soil organic matter content (p < 0.0001). Overall, cacao had more favorable physical, biological and chemical soil characteristics than banana and pineapple monocultures, while trends were less conclusive compared to the pastureland. While organic cacao was inferior to native forest in some soil characteristics such as bulk density and organic carbon, its soil quality did best mimic that of the native forest. This supports the organic cultivation of cacao as a desirable alternative to banana and pineapple monoculture.

  12. Dieta del capibara Hydrochaeris hydrochaeris (Rodentia: Hydrochaeridae en Caño Limón, Arauca, Colombia

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    Jimena Forero-Montaña

    2003-06-01

    Full Text Available Estudiamos la composición y variación estacional de la dieta del chigüiro (Hydrochaeris hydrochaeris en los esteros de Caño Limón, por medio de observaciones directas del consumo de las plantas. Los chigüiros se alimentaron exclusivamente de plantas y su dieta estuvo compuesta por 89 especies, pertenecientes a 22 familias. El 63 % de las especies son nuevos registros para la dieta del chigüiro. Las cuatro familias mas consumidas fueron Poaceae, Cyperaceae, Leguminosae y Pontederiaceae, las cuales representaron el 94% del total de la dieta. Solo siete especies representaron el 60% del total de la dieta, así: los pastos Hymenachne amplexicaulis (16.9%, Digitaria bicornis (10.9%, Paspalum fasciculatum (10.9%, Cynodon dactylon (6.8%, Urochloa arrecta (4.5% y Panicum maximum (4.4% y la ciperácea Rynchospora corymbosa (4.4%. Se presentó variación en la composición de la dieta de los chigüiros durante las épocas de muestreo.We studied the composition and seasonal variation of the diet of the capybara (Hydrochaeris hydrochaeris in the flooded savannas of Caño Lim��n, Colombia. This was achieved by direct observation of the consumption patterns of these animals. The capybaras only consumed plants, and their diet included 89 species of 22 families. Sixty three percent of these plant species had not been reported before. The most commonly consumed plants (94% of the diet, belonged to the Poaceae, Cyperaceae, Leguminosae and Pontederiaceae. Only seven species represented 60% of the total diet: the grasses Hymenachne amplexicaulis (16.9%, Digitaria bicornis (4.5% and Panicum maximum (4.4% and the Cyperaceae Rynchospora corymbosa (4.4%. There was seasonal variation in the diet composition of capybaras.

  13. A Request for the Conference and Symposia Grant from COMP Division of American Chemical Society

    Science.gov (United States)

    2015-03-02

    characterization of the fluorite/water interface from theory and experiments Rémi Khatib, Maria J Perez-Haro, Ellen HG Backus, Marie-Pierre Gaigeot...Hyungjun Kim, Hyung-Kyu Lim, Hyeyoung Shin, William A Goddard III. 4:45 pm 461 Modifying MOFs for specific adsorption interactions: The cases of H2 and

  14. The structure of an LIM-only protein 4 (LMO4 and Deformed epidermal autoregulatory factor-1 (DEAF1 complex reveals a common mode of binding to LMO4.

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    Soumya Joseph

    Full Text Available LIM-domain only protein 4 (LMO4 is a widely expressed protein with important roles in embryonic development and breast cancer. It has been reported to bind many partners, including the transcription factor Deformed epidermal autoregulatory factor-1 (DEAF1, with which LMO4 shares many biological parallels. We used yeast two-hybrid assays to show that DEAF1 binds both LIM domains of LMO4 and that DEAF1 binds the same face on LMO4 as two other LMO4-binding partners, namely LIM domain binding protein 1 (LDB1 and C-terminal binding protein interacting protein (CtIP/RBBP8. Mutagenic screening analysed by the same method, indicates that the key residues in the interaction lie in LMO4LIM2 and the N-terminal half of the LMO4-binding domain in DEAF1. We generated a stable LMO4LIM2-DEAF1 complex and determined the solution structure of that complex. Although the LMO4-binding domain from DEAF1 is intrinsically disordered, it becomes structured on binding. The structure confirms that LDB1, CtIP and DEAF1 all bind to the same face on LMO4. LMO4 appears to form a hub in protein-protein interaction networks, linking numerous pathways within cells. Competitive binding for LMO4 therefore most likely provides a level of regulation between those different pathways.

  15. The Experience of Laboratory Information Management System Selection%实验室信息管理系统(LIMS)选型经验

    Institute of Scientific and Technical Information of China (English)

    林伟强

    2008-01-01

    选型作为实验室信息管理系统(LIMS)项目的第一步,是实施、应用的基础,对整个项目的成败起着至关重要的作用.广东省药品检验实验室信息管理系统(GDIDC-LJMS)选型工作从2005年初开始,历时近一年.本文结合我单位项目的工作,介绍LIMS选型的经验和体会.

  16. Propuesta para la creación de un centro de compras de farmacias en la provincia de Limón

    OpenAIRE

    Lao-Joa, Ana-Isabel

    2009-01-01

    Tesis de maestría -- Universidad de Costa Rica. Posgrado en Administración y Dirección de Empresas. Maestría Profesional en Administración y Dirección de Empresas, 2009 El objetivo general del trabajo es proponer la creación de un centro de compras de farmacias en la provincia de Limón con la finalidad de obtener mejores descuentos en los medicamentos y poder competir contra las grandes cadenas. La organización investigada es una farmacia y se dedica a la venta de medicamentos. ...

  17. Heterogenic final cell cycle by chicken retinal Lim1 horizontal progenitor cells leads to heteroploid cells with a remaining replicated genome.

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    Shahrzad Shirazi Fard

    Full Text Available Retinal progenitor cells undergo apical mitoses during the process of interkinetic nuclear migration and newly generated post-mitotic neurons migrate to their prospective retinal layer. Whereas this is valid for most types of retinal neurons, chicken horizontal cells are generated by delayed non-apical mitoses from dedicated progenitors. The regulation of such final cell cycle is not well understood and we have studied how Lim1 expressing horizontal progenitor cells (HPCs exit the cell cycle. We have used markers for S- and G2/M-phase in combination with markers for cell cycle regulators Rb1, cyclin B1, cdc25C and p27Kip1 to characterise the final cell cycle of HPCs. The results show that Lim1+ HPCs are heterogenic with regards to when and during what phase they leave the final cell cycle. Not all horizontal cells were generated by a non-apical (basal mitosis; instead, the HPCs exhibited three different behaviours during the final cell cycle. Thirty-five percent of the Lim1+ horizontal cells was estimated to be generated by non-apical mitoses. The other horizontal cells were either generated by an interkinetic nuclear migration with an apical mitosis or by a cell cycle with an S-phase that was not followed by any mitosis. Such cells remain with replicated DNA and may be regarded as somatic heteroploids. The observed heterogeneity of the final cell cycle was also seen in the expression of Rb1, cyclin B1, cdc25C and p27Kip1. Phosphorylated Rb1-Ser608 was restricted to the Lim1+ cells that entered S-phase while cyclin B1 and cdc25C were exclusively expressed in HPCs having a basal mitosis. Only HPCs that leave the cell cycle after an apical mitosis expressed p27Kip1. We speculate that the cell cycle heterogeneity with formation of heteroploid cells may present a cellular context that contributes to the suggested propensity of these cells to generate cancer when the retinoblastoma gene is mutated.

  18. Injury-independent induction of reactive gliosis in retina by loss of function of the LIM homeodomain transcription factor Lhx2

    OpenAIRE

    2012-01-01

    Müller glia are the primary glial subtype in the retina and perform a wide range of physiological tasks in support of retinal function, but little is known about the transcriptional network that maintains these cells in their differentiated state. We report that selective deletion of the LIM homeodomain transcription factor Lhx2 from mature Müller glia leads to the induction of reactive retinal gliosis in the absence of injury. Furthermore, Lhx2 expression is also down-regulated in Prph2Rd2/R...

  19. Análisis de la oferta y demanda del sector hotelero del Cantón Limón Indanza

    OpenAIRE

    Peñafiel Iñiguez, Xavier Geovanny

    2015-01-01

    El análisis de las fuerzas del mercado del sector hotelero en el Cantón Limón Indanza se lo realizó mediante una investigación analítica y comparativa entre las características de los establecimientos y los huéspedes por el número de pernoctaciones y otros factores subyacentes; utilizando métodos cuantitativos y cualitativos como: encuestas, hojas de planta, fichas de registro y otros formularios con el fin de realizar un diagnóstico de la situación actual del servicio de alojamiento, permiti...

  20. Professional LIMS Delivered On-Demand%按需交付的专业化实验室信息管理系统

    Institute of Scientific and Technical Information of China (English)

    Steven CHEN; Thomas KENT

    2010-01-01

    使用SciLIMS SSM,无论样品存放在何处,几乎所有实验室都可以跟踪成千甚至上万的样品.它是一种十分便利的实验室信息管理系统,即使是最小的实验室也可以拥有.此外,它还有一个优点即十分容易使用.

  1. Mouse transgenesis identifies conserved functional enhancers and cis-regulatory motif in the vertebrate LIM homeobox gene Lhx2 locus.

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    Alison P Lee

    Full Text Available The vertebrate Lhx2 is a member of the LIM homeobox family of transcription factors. It is essential for the normal development of the forebrain, eye, olfactory system and liver as well for the differentiation of lymphoid cells. However, despite the highly restricted spatio-temporal expression pattern of Lhx2, nothing is known about its transcriptional regulation. In mammals and chicken, Crb2, Dennd1a and Lhx2 constitute a conserved linkage block, while the intervening Dennd1a is lost in the fugu Lhx2 locus. To identify functional enhancers of Lhx2, we predicted conserved noncoding elements (CNEs in the human, mouse and fugu Crb2-Lhx2 loci and assayed their function in transgenic mouse at E11.5. Four of the eight CNE constructs tested functioned as tissue-specific enhancers in specific regions of the central nervous system and the dorsal root ganglia (DRG, recapitulating partial and overlapping expression patterns of Lhx2 and Crb2 genes. There was considerable overlap in the expression domains of the CNEs, which suggests that the CNEs are either redundant enhancers or regulating different genes in the locus. Using a large set of CNEs (810 CNEs associated with transcription factor-encoding genes that express predominantly in the central nervous system, we predicted four over-represented 8-mer motifs that are likely to be associated with expression in the central nervous system. Mutation of one of them in a CNE that drove reporter expression in the neural tube and DRG abolished expression in both domains indicating that this motif is essential for expression in these domains. The failure of the four functional enhancers to recapitulate the complete expression pattern of Lhx2 at E11.5 indicates that there must be other Lhx2 enhancers that are either located outside the region investigated or divergent in mammals and fishes. Other approaches such as sequence comparison between multiple mammals are required to identify and characterize such enhancers.

  2. Effects of different agricultural systems on soil quality in Northern Limón province, Costa Rica

    Directory of Open Access Journals (Sweden)

    Emma Cornwell

    2014-09-01

    Full Text Available Conversion of native rainforest ecosystems in Limón Province of Costa Rica to banana and pineapple monoculture has led to reductions in biodiversity and soil quality. Agroforestry management of cacao (Theobroma cacao is an alternative system that may maintain the agricultural livelihood of the region while more closely mimicking native ecosystems. This study compared physical, biological and chemical soil quality indicators of a cacao plantation under organic agroforestry management with banana, pineapple, and pasture systems; a native forest nearby served as a control. For bulk density and earthworm analysis, 18 samples were collected between March and April 2012 from each ecosystem paired with 18 samples from the cacao. Cacao had a lower bulk density than banana and pineapple monocultures, but greater than the forest (p<0.05. Cacao also hosted a greater number and mass of earthworms than banana and pineapple (p<0.05, but similar to forest and pasture. For soil chemical characteristics, three composite samples were collected in March 2012 from each agroecosystem paired with three samples from the cacao plantation. Forest and pineapple ecosystems had the lowest pH, cation exchange capacity, and exchangeable nutrient cations, while cacao had the greatest (p<0.05. Total nutrient levels of P and N were slightly greater in banana, pineapple and pasture than in cacao; probably related to addition of chemical fertilizer and manure from cattle grazing. Forest and cacao also had greater %C, than other ecosystems, which is directly related to soil organic matter content (p<0.0001. Overall, cacao had more favorable physical, biological and chemical soil characteristics than banana and pineapple monocultures, while trends were less conclusive compared to the pastureland. While organic cacao was inferior to native forest in some soil characteristics such as bulk density and organic carbon, its soil quality did best mimic that of the native forest. This

  3. Application of SoLIM Based on Case Reasoning in Soil Nutrient Mapping%基于案例推理的SoLIM方法在土壤养分制图中的应用

    Institute of Scientific and Technical Information of China (English)

    徐丽华; 谢德体; 魏朝富; 李兵

    2013-01-01

    The 111 soil samples were collected in Wangjiagou watershed of Three Gorges Reservoir Area with complex geographical environment.Total nitrogen content in the soil was measured,and the land use data and elevation,slope,plan curvature,profile curvature,topographic position index and topographic wetness index were collected.The soil total nitrogen was mapped by using the soil land inference model (SoLIM) that is based on case reasoning.The results showed that,land use type data can be used to improve the accuracy of prediction.The spatial distribution of soil nutrients produced by the SoLIM contained more information,and presented more details of spatial variability of soil nutrients.Therefore,the SoLIM based on case reasoning was validated a good method for soil nutrient mapping in the small study area with complex geographical environment.

  4. Tissue-specific regulation of the LIM homeobox gene lin-11 during development of the Caenorhabditis elegans egg-laying system.

    Science.gov (United States)

    Gupta, Bhagwati P; Sternberg, Paul W

    2002-07-01

    The egg-laying system of Caenorhabditis elegans hermaphrodites requires development of the vulva and its precise connection with the uterus. This process is regulated by LET-23-mediated epidermal growth factor signaling and LIN-12-mediated lateral signaling pathways. Among the nuclear factors that act downstream of these pathways, the LIM homeobox gene lin-11 plays a major role. lin-11 mutant animals are egg-laying defective because of the abnormalities in vulval lineage and uterine seam-cell formation. However, the mechanisms providing specificity to lin-11 function are not understood. Here, we examine the regulation of lin-11 during development of the egg-laying system. Our results demonstrate that the tissue-specific expression of lin-11 is controlled by two distinct regulatory elements that function as independent modules and together specify a wild-type egg-laying system. A uterine pi lineage module depends on the LIN-12/Notch signaling, while a vulval module depends on the LIN-17-mediated Wnt signaling. These results provide a unique example of the tissue-specific regulation of a LIM homeobox gene by two evolutionarily conserved signaling pathways. Finally, we provide evidence that the regulation of lin-11 by LIN-12/Notch signaling is directly mediated by the Su(H)/CBF1 family member LAG-1.

  5. Contexto y Caracterización de la Cadena de Suministro del Limón Persa (Citrus latifolia Tanaka en Veracruz-México

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    Gregorio Fernández-Lambert

    2015-01-01

    Full Text Available Esta investigación se realiza en el Distrito Citrícola de Martínez de la Torre, Ver., con alcance a los municipios de Álamo Temapache, y Cuitláhuac en Veracruz, y reporta el contexto e integración de la cadena de suministro del limón persa. El estudio de sus eslabones permitió identificar las problemáticas de abastecimiento, operación y distribución en dicha cadena. A partir de entrevistas abiertas no estructuradas a especialistas, técnicos, productores y empresarios, así como la consulta de bases de datos y documentos de organismos afines a esta agroindustria, los resultados de la investigación advierten que la fuente de los problemas estructurales de la ineficacia en la sincronización de la cadena de suministro del limón persa, se centra en la baja productividad del huerto, un excesivo intermediarismo entre el huerto y la exportadora, la baja adopción tecnológica o uso de tecnología hechiza en los procesos de empaque del cítrico.

  6. De solidaridades problemáticas y utopías sin armonizar: una lectura de Limón Reggae (2007), de Anacristina Rossi, y Big Banana (2000), de Roberto Quesada

    OpenAIRE

    Rios Quesada, Veronica

    2016-01-01

    This article analyzes the limits of solidarity based on the decentering of the mestizo protagonists in Limón Reggae by Costa Rican writer Anacristina Rossi (2007), which portrays the Costa Rican descendants of immigrants from the Antilles (primarily from Jamaica), and Big Banana by Honduran author Roberto Quesada (2000), which describes the Garifuna people from Honduras. While Limón Reggae explores a solidarity that is both political and structural in nature in both Costa Rica and the United ...

  7. 基于LIMS数据库的环境监测数据统计方法——以上海市嘉定区实验室信息系统为例%Statistical Method of Environmental Monitoring Data Based on LIMS Database——Taking the Laboratory Information Management System Used in Jiading,Shanghai as Example

    Institute of Scientific and Technical Information of China (English)

    曹升华

    2013-01-01

    Laboratory Information Management System(LIMS) has been disseminated in the area of environmental monitoring/There are huge datas and infomations of environmental monitoring in the database of LIMS.Compared with traditional statistical method, we can greatly improve the efficiency and accuracy of statistics by using the LIMS database, according to SELECT operate the LIMS with SQL sentence.%在环境监测领域中,实验室信息管理系统(Laboratory Information Management System,LIMS)已经逐渐地得到了普及,在LIMS数据库中涵盖了大量的环境监测信息和数据,与传统的数据统计方法相比,通过SQL语句对LIMS数据库进行SELECT操作,能够大大提高统计的效率和准确性.

  8. Produção de mudas de capim-limão em diferentes recipientes e substratos Transplant production of lemon grass in different recipients and substrates

    Directory of Open Access Journals (Sweden)

    Arie F Blank

    2009-12-01

    Full Text Available Os efeitos de recipientes e misturas de substratos, diâmetros de perfilho e comprimentos de lâmina foliar mantida no perfilho foram avaliados para produção de mudas de capim-limão (Cymbopogon citratus (D.C. Stapf.. No primeiro ensaio testaram-se dois recipientes e nove misturas de substratos. Os substratos avaliados foram pó de coco, casca de arroz carbonizada e esterco bovino na proporção 1:1:2; pó de coco, casca de arroz carbonizada, solo e esterco bovino na proporção 1:1:1:3. O recipiente tubete de 110 cm³ foi melhor que a bandeja de poliestireno expandido com 72 células de 121,2 cm³ para a produção de mudas de capim-limão. Todos os substratos testados resultaram na produção de mudas de capim-limão de boa qualidade. No segundo ensaio, foram testados dois diâmetros de perfilho (>1,5 e The effect of recipients, substrate mixtures, diameters of tillers and of different leaf lengths kept on the tiller was evaluated for transplant production of lemon grass (Cymbopogon citratus (D.C. Stapf.. In the first assay, we tested two recipients and nine mixtures of substrates. The tublet recipient of 110 cm3 was better than the expanded polystyrene foam bed with 72 cells of 121.2 cm3 and all the tested substrates (coconut dust, carbonized rice hulls and bovine manure at rates of 1:1:2, and coconut dust, carbonized rice hulls, soil and bovine manure at rates of 1:1:1:3 resulted in good quality of lemon grass transplants. In the second assay, we tested two tiller diameters (>1.5 and <1.5 cm and the maintenance of four leaf lengths on tiller (0, 3, 6 and 9 cm. It was concluded that if tillers were thicker than 1.5 cm diameter, then 5.5 cm of leaf length should be kept and if the tiller diameter were inferior to 1.5 cm, then the total leaf area should be cut off in order to promote tilling.

  9. Potencial antioxidante de extratos de sementes de limão (Citrus limon Antioxidant potential of lemon seed extracts (Citrus limon

    Directory of Open Access Journals (Sweden)

    Débora Maria Moreno Luzia

    2010-06-01

    Full Text Available O presente trabalho teve como principais objetivos avaliar o comportamento do óleo de soja acrescido de extratos de sementes de limão (Citrus limon, em diferentes concentrações, por meio da estabilidade oxidativa e medir a atividade antioxidante através do método do radical livre DPPH e compostos fenólicos totais. A concentração de 2.400 mg.kg-1 para o extrato de sementes de limão, variedade galego, foi a que conferiu melhor estabilidade oxidativa ao óleo de soja. As atividades antioxidantes máximas e os valores da concentração de extrato natural suficientes para obter 50% do efeito máximo, estimado em 100% (EC50, determinados pelo DPPH para o extrato e ácido gálico foram 70,58%, 69,94 μg.mL-1 e 75,07%, 64,73 μg.mL-1, respectivamente. A concentração de compostos fenólicos totais, determinada pelo método de Folin-Ciocalteu foi de 76 mg.g-1. Foi possível concluir que o extrato de sementes de limão galego possui ação antioxidante natural, podendo ser aplicado em alimentos.The present study aimed at evaluating the behavior of the soybean oil with the addition of lemon seed extracts (Citrus limon, in different concentrations, through oxidative stability and also at measuring the antioxidant activity using the DPPH free radical method and total phenolic compounds. The concentration of 2,400 mg.kg-1 for the lemon seed extract, galego variety, was the one that provided the soybean oil with the best oxidative stability. The maximum antioxidant activities and the concentration values of the natural extract sufficient to obtain 50% of maximum effect, estimated at 100% (EC50, determined by DPPH for the extract and gallic acid were 70.58%, 69.94 μg.mL-1 and 75.07%, 64.73 μg.mL-1, respectively. The concentration of total phenolic compounds, determined by the Folin-Ciocalteu method, was of 76 mg.g-1. Thus, it can be said that the lemon seed extract, galego, presents natural antioxidant action demonstrating potential to be used in

  10. Estructura de la población y distribución de talla del ostión de manglar (Crassostrea rhizophorae, Guilding, 1828), en el Estero Vizcaya, Limón, Costa Rica

    OpenAIRE

    Madrigal Castro, Eduardo; Alfaro Montoya, Jorge; Quesada Quesada, Rodolfo; Pacheco Urpí, Oscar; Zamora Madriz, Eduardo

    2016-01-01

    A quantitative study of Crassostrea rhizophorae in Estero Vizcaya, Limón, Costa Rica, during December 1983, showed a population of 19,760 oysters with a confidence limit of 90% and an error of ±100 oysters. Of this population, 43.53% were over 60.0 mm in length (the commercial size established for this area).

  11. Primeira ocorrência de ferrugem em capim-limão causada por Puccinia cymbopogonis no Brasil First report of the lemongrass rust fungi caused by Puccinia cymbopogonis in Brazil

    Directory of Open Access Journals (Sweden)

    João Batista Vida

    2006-03-01

    Full Text Available È registrada a primeira ocorrência de ferrugem em capim-limão (Cymbopogon citratus (DC. Stapf, causada por Puccinia cymbopogonis Mass., no Brasil, Estado do Paraná.It is reported the first occurrence of the lemongrass (Cymbopogon citratus (DC. Stapf rust fungi caused by Puccinia cymbopogonis Mass. in Brazil, State of Paraná.

  12. LIMS and construction of digitalized quality inspection laboratory%LIMS系统及数字化质检实验室建设

    Institute of Scientific and Technical Information of China (English)

    齐尚忠

    2009-01-01

    The situation and development trends of laboratory information management system (LIMS) are introduced. Problems in the construction of digitalized quality inspection laboratory are discussed, and proposals are given for the construction of a world-oriented digitalized laboratory.%本文首先对LIMS系统的国内外发展情况进行了总结,展望了其发展趋势,然后分析了质检实验室在数字化建设方面存在的一些问题,并就其如何顺应以上发展趋势,建设面向世界的数字化实验室提出了一些建议.

  13. Estudio de réplicas del Terremoto de Limón usando datos locales: resultados e implicaciones tectónicas

    OpenAIRE

    Ponce, Lautaro; Rodríguez, Jaime; Domínguez, Jaime; Montero, Walter; Rojas, Wilfredo; Boschini, Ileana; Suárez, Gerardo; Camacho, Eduardo

    2010-01-01

    Un estudio de réplicas del Terremoto de Limón del 22 de abril de 1991 (M= 7,6) fue realizado combinando datos de una red temporal de estaciones ubicadas dentro del área epicentral, en conjunto con datos obtenidos de la Red Sismológica Nacional (RSN, ICE-UCR). El estudio permitió la definición del área de ruptura frágil el cual se estima en 85 x 45 km2 y del ángulo promedio de inclinación de la falla principal, el cual se considera es de 23° al SW. Además se encuentra que la falla se hace más ...

  14. Distribucion arborea de los gastropodos cerithidea montagnei (mollusca potamidae y littorina Spp. (Mollusca, Littorinidae en el manglar de Mata de Limón, Costa Rica

    Directory of Open Access Journals (Sweden)

    Édgar Suarez B

    2016-03-01

    Full Text Available En el manglar de Mata de Limón, Puntarenas, Costa Rica, se estudió la distribución y preferencia por el susbtrato arbóreo de gastrópodos C. montagnei y Littorina spp. en cuatro especies de mangle, según la cantidad de moluscos observados en cada árbol y la cantidad de árboles con presencia o ausencia de ellos, en el período de setiembre de 1982 a junio de 1983. El orden de preferencia mostrado por ambos grupos de moluscos fue: 1 Rhizophora mangle, 2 Avicennia germinans, 3Pelliciera rhizophorae y 4 Laguncularia racemosa. Se discuten las posibles razones de esta preferencia. Cerithidea montagnei presentó una mayor distribución horizontal, así como una mayor abundancia respecto de Littorina spp.

  15. Descripción de un nuevo limácido de Menorca (Islas Baleares: Gigantomilax (Vitrinoides benjaminus sp. n. (Gastropoda, Pulmonata

    Directory of Open Access Journals (Sweden)

    Borredà, V.

    2008-06-01

    Full Text Available Descripción de un nuevo limácido de Menorca (Islas Baleares: Gigantomilax (Vitrinoides benjaminus sp. n. (Gastropoda, Pulmonata We describe a new endemic limacid slug, Gigantomilax (Vitrinoides benjaminus n. sp. and we compare it with the nearest species, particularly with Gigantomilax (V. majoricensis, another Balearic endemism. Characteristic features of this new species include its small size, translucid and smooth body, and totally grey dorsum with no bands or spots. The reproductive system shows a well developed vas deferens surrounded by the distal part of prostatic gland, a thin penial retractor muscle, swollen prostate and small distal genitalia. The recent redescription of Limax majoricensis by Wiktor et al. (2007 is discussed and we consider that these authors refer to G. benjaminus n. sp., and so we dessign the neotype of Gigantomilax (V. majoricensis.

  16. ambiental de agricultores orgánicos y convencionales en el ejido La Ciénega, municipio de El Limón, Jalisco, México

    Directory of Open Access Journals (Sweden)

    Rodolfo González-Figueroa

    2007-01-01

    Full Text Available La agricultura moderna en México está causando contaminación y erosión; de ello deriva la creciente pobreza y marginación que se padece en diversas zonas rurales, donde la cultura y el sustento dependen de la agricultura. Entender y responder al fenómeno de la degradación ambiental implica comprender las ideas, valores y esperanzas de los campesinos y, a partir de ello, construir alternativas. En este artículo se presentan cuatro estudios de caso en el ejido de La Ciénega, municipio El Limón, en la región Costa Sur de Jalisco, México, buscando contextualizar estos casos en el debate sobre el desarrollo sustentable.

  17. Parasitosis intestinal en monos capuchinos cariblancos Cebus capucinus (Primates: Cebidae de un área protegida en la provincia de Limón, noreste de Costa Rica

    Directory of Open Access Journals (Sweden)

    Misael Chinchilla

    2010-12-01

    Full Text Available La deforestación de bosques tropicales está amenazando la biodiversidad de las especies de primates y su estado de salud, dependiente de un lugar ecológicamente equilibrado. Con el fin de evaluar esta relación, se analizó la presencia de parásitos en los intestinos de esta especie. El estudio se realizó en la Estación Biológica La Suerte (EBLS, Limón, Costa Rica. El grupo de monos capuchinos cariblancos (Cebus capucinus fue observado entre marzo y diciembre de 2006. Se recolectaron 75 muestras de heces a las que se les identificó las plantas ingeridas, y se procesaron en el laboratorio para evaluar la prevalencia de parásitos. Moraceae fue la familia de plantas más reportada. Strongyloides spp. y acantocéfalos fueron los más comunes. La distribución positiva de las mismas fue similar independientemente del sexo y la edad. Microsporidios se reportaron mayoritariamente en heces asociadas con la familia Piperaceae. Fue encontrada una baja existencia de éstos parásitos en muestras asociadas con Myrtaceae en cuyo género Psidium, se han reportado compuestos activos antiparasitarios. La aparición de parásitos fue relativamente mayor en la EBLS, comparado con muestras de otras regiones más secas de Costa Rica. Por lo tanto, esa expresión parasitaria puede deberse al hecho de ser la EBLS una selva lluviosa, además de estar rodeada por zonas con actividades antrópicas. Se sugiere incrementar las investigaciones de parasitología de campo en primates neotropicales con el fin de entender plenamente las relaciones parásitos-hospederos, para en un largo plazo poder comprender los ecosistemas donde conviven, y en consecuencia, preservar la diversidad biológica.Intestinal parasites in white-faced capuchin monkeys Cebus capucinus Primates: Cebidae inhabiting a protected area in the Limón province of Northeastern Costa Rica. Deforestation of tropical forests is threatening monkey biodiversity and their health status, dependent of an

  18. Corrosión atmosférica en ambientes abrigados en Limón, Costa Rica, en el marco del proyecto TROPICORR

    Directory of Open Access Journals (Sweden)

    Juan Fernando Alvarez

    2008-04-01

    Full Text Available Se estudia la corrosión atmosférica en ambientes abrigados (INDOOR en Limón, Puerto en el Atlántico de Costa Rica, de diferentes materiales utilizados en la industria electro-electrónica (acero, cobre, estaño y zinc y se compara con valores de corrosión en las mismas condicionesen otras zonas. El estudio está en el marco del proyecto internacional denominado TROPICORR del CYTED, en el cual se participa, y que tiene como interés estudiar la corrosión de materiales metálicos utilizados en la industria electro-electrónica en ambientes tropicales. Las condicionesque afectan a la corrosión atmosférica son: la temperatura, la humedad, el tiempo de humectación (donde la temperatura es mayor a 0 ºC y 80% HR y contaminantes, especialmente cloruros y SO2. En el caso del presente estudio estos efectos son evidentes en Costa Rica, ubicada en la latitud 10º Norte, y por su ubicación geográfica, entre dos océanos y con una formación montañosa en el centro del país, en donde se encuentran varios volcanes.En Limón, se tienen temperaturas mayores a 0 ºC todo el año y humedades elevadas, así como contaminantes de cloruros por estar situado en la costa y además, en la estación del estudio SO2 por efectos particulares. La metodología de estudio corresponde al tipo 3-4 de la norma ETS y a la norma ISO 85653, y se presentan los resultados obtenidos en tres años deestudio. También se compara tanto para el acero como para el cobre el efecto de ser expuesta las muestras tanto a la intemperie como en condiciones abrigadas.

  19. Neogene-quaternary Ostracoda and paleoenvironments, of the Limón basin, Costa Rica, and Bocas del Toro basin, Panama

    Science.gov (United States)

    Borne, P.F.; Cronin, T. M.; Hazel, J.E.

    1999-01-01

    Tropical marine ostracodes from Neogene and Quaternary sediments of the Central American Caribbean region have been the subject of biostratigraphic, ecological, taxonomic, and evolutionary studies. As part of the Panama Paleontology Project (PPP), Neogene and Quaternary ostracodes are being studied from the Central American region. The overall goal of this research is to evaluate the impact of the emergence of the Central American Isthmus as a land barrier between the Caribbean/tropical Atlantic and the Pacific oceans on marine ostracode biodiversity and the oceanic environments in which extant ostracodes evolved. Due to the ecological specificity of many living tropical ostracode species, they are ideally suited for reconstructing paleoenvironments on the basis of their occurrence in fossil assemblages, which in turn can lead to a better understanding of the tropical climatic and tectonic history of Central America. The principal aims of this chapter are: (a) to document the composition of the ostracode assemblages from the Limón Basin of Costa Rica and the Bocas del Toro Basin of Panama, two areas yielding extensive ma rine ostracode assemblages; (b) to describe the environments of deposition within these basins; and (c) to document the stratigraphic distribution of potentially agediagnostic ostracode species in the Limón and Bocas del Toro basins in order to enhance their use in Central American biostratigraphy. A secondary, but none-the-less important goal is to assemble a database on the distribution of modem ostracode species in the Caribbean and adjacent areas as a basis for comparison with fossil assemblages. Although the ecological, biostratigraphic and paleoenvironmental conclusions presented here will improve as additional material is studied, these fossil and modem ostracode databases constitute the foundation for future evolutionary and geochernical studies of tropical Caribbean and eastern Pacific Ocean ostracodes. Moreover, we present here evidence

  20. Análisis comparativo de sustratos para colectar juveniles del ostión de manglar (Crassostrea rhizophorae, Guilding 1828) en Estero Vizcaya, Limón, Costa Rica

    OpenAIRE

    Alfaro Montoya, Jorge; Quesada Quesaada, Rodolfo; Zamora Madriz, Eduardo; Madrigal Castro, Eduardo; Pacheco Urpí, Oscar

    2016-01-01

    An experiment for evaluating the best substrate for collecting Crassostrea rhizophorae spat, using plates of asbestos-cement, rubber tire tubes, oyster shells, plywood, plastic bottles and plexiglass, was carried out from March to April 1984 (dry season) in Estero Vizcaya, Limón, Costa Rica.The plexiglass and plastic bottles were more efficient for removal of small oyster seed, and asbestoscement and rubber tire tubes gave the best results for oyster settlement per m2. Settlement of undesirab...

  1. Determinación del coeficiente global de transferencia de calor en secaderos rotativos de cáscara de limón Determination of global heat transfer coefficient in lemon peel rotary dryers

    Directory of Open Access Journals (Sweden)

    Marcos A. Golato

    2005-12-01

    Full Text Available En la bibliografía consultada no se encontraron trabajos de secado de cáscara de limón en secaderos rotativos. Se realizaron ensayos de medición en secaderos rotativos de cáscara de limón en una instalación agroindustrial del noroeste argentino. Se desarrolló un modelo matemático semiempírico mediante la resolución de los balances de materia y energía. Se obtuvo una correlación estadística para la predicción de los coeficientes globales volumétricos de transferencia de calor aparentes para secaderos rotativos de cáscara de limón.No papers on lemon peel drying in rotary dryers were actually found in the sources consulted. Measurement tests were carried out in the assessment of rotary dryer systems used to dehydrate lemon peel in an agroindustrial plant of Northwestern Argentina. A semiempirical mathematic model was developed solving mass and energy balances. A statistical correlation for predicting global heat transfer coefficient in rotary dryer lemon peel dehydration was obtained.

  2. Caracterização dos óleos das sementes de limão rosa (Citrus limonia Osbeck e limão siciliano (Citrus limon, um resíduo agroindustrial Characterization of rangpur lime (Citrus limonia Osbeck and "sicilian" lemon (Citrus limon seed oils, an agroindustrial waste

    Directory of Open Access Journals (Sweden)

    Seme Youssef Reda

    2005-12-01

    Full Text Available A finalidade deste estudo foi caracterizar os óleos extraídos das sementes de limão rosa e limão siciliano, como aproveitamento de resíduos industriais. Foram determinados os índices de iodo, ácidos graxos livres (AGL,% em ácido oléico e teor de peróxidos. Foram realizadas análises termoanalíticas (termogravimetria, TG; termogravimetria diferencial, DTG e calorimetria diferencial exploratória, DSC; espectroscópicas (infravermelho e ultravioleta e cromatográficas (cromatografia gasosa. Foram identificados no óleo das sementes de limão rosa os seguintes ácidos graxos na forma de ésteres metílicos: caprílico (0,90%, palmítico (21,40%, esteárico (2,60%, oléico (21,20%, linoléico (43,00%, linolênico (7,60% e araquídico (0,20% e no óleo das sementes de limão siciliano: caprílico (1,00%, mirístico (0,10%, palmítico (19,60%, esteárico (3,00%, oléico (28,60%, linoléico (34,40%, linolênico (10,00% e araquídico (0,20%. Os resultados indicaram que ambos os óleos apresentam estabilidade térmica até 250ºC, composição química semelhante e alto teor de ácidos graxos insaturados (71,80 e 73,00%. Pela composição de ácidos graxos também foi possível determinar a provável composição em triacilgliceróis dos óleos. Os resultados mostraram que os óleos apresentam características semelhantes a óleos comestíveis de boa qualidade.The purpose of this study was to characterize the oil extracted from rangpur lime and "Sicilian" lemon seeds. Iodine, peroxide values, as well as free fatty acid percent (FFA% expressed as oleic acid were accomplished. The thermal gravimetric analysis, TG; differential thermal gravimetric, DTG; differential scanning calorimetry analysis, DSC; infrared and ultraviolet spectroscopic and gas chromatographic analysis were also determined. In the rangpur lime seed oil, the following fatty acids, as methyl esters were identified: caprilic (0,90%, palmitic (21,40%, stearic (2,60%, oleic (21

  3. Four and a Half LIM Domains 1b (Fhl1b) Is Essential for Regulating the Liver versus Pancreas Fate Decision and for β-Cell Regeneration

    Science.gov (United States)

    Xu, Jin; Cui, Jiaxi; Del Campo, Aranzazu; Shin, Chong Hyun

    2016-01-01

    The liver and pancreas originate from overlapping embryonic regions, and single-cell lineage tracing in zebrafish has shown that Bone morphogenetic protein 2b (Bmp2b) signaling is essential for determining the fate of bipotential hepatopancreatic progenitors towards the liver or pancreas. Despite its pivotal role, the gene regulatory networks functioning downstream of Bmp2b signaling in this process are poorly understood. We have identified four and a half LIM domains 1b (fhl1b), which is primarily expressed in the prospective liver anlage, as a novel target of Bmp2b signaling. fhl1b depletion compromised liver specification and enhanced induction of pancreatic cells from endodermal progenitors. Conversely, overexpression of fhl1b favored liver specification and inhibited induction of pancreatic cells. By single-cell lineage tracing, we showed that fhl1b depletion led lateral endodermal cells, destined to become liver cells, to become pancreatic cells. Reversely, when fhl1b was overexpressed, medially located endodermal cells, fated to differentiate into pancreatic and intestinal cells, contributed to the liver by directly or indirectly modulating the discrete levels of pdx1 expression in endodermal progenitors. Moreover, loss of fhl1b increased the regenerative capacity of β-cells by increasing pdx1 and neurod expression in the hepatopancreatic ductal system. Altogether, these data reveal novel and critical functions of Fhl1b in the hepatic versus pancreatic fate decision and in β-cell regeneration. PMID:26845333

  4. LIMS中样品检测报告模板的开发设计%Development and design of sample test report template in LIMS

    Institute of Scientific and Technical Information of China (English)

    林彤

    2016-01-01

    Physical properties analysis and detection is an important means of product quality control, performance evaluation and failure analysis in rubber plastic sealing industry. It plays an important role in rubber&plastic product development process. The application of LIMS in central laboratory standardized the test report output process, automated data collection and test re-ports outputting process, improved the overall level and efficiency of the laboratory management.%物理性能分析、检测是橡塑密封制品检测中对产品质量、性能评价、失效分析的重要手段,在橡塑类产品研制过程中发挥着重要作用。中心实验室对过实施应用LIMS,规范了报告输出业务流程,实现了数据自动采集,检测报告的全自动生成,提高了实验室整体管理水平和工作效率。

  5. Modulation of light-driven arousal by LIM-homeodomain transcription factor Apterous in large PDF-positive lateral neurons of the Drosophila brain.

    Science.gov (United States)

    Shimada, Naoto; Inami, Show; Sato, Shoma; Kitamoto, Toshihiro; Sakai, Takaomi

    2016-11-17

    Apterous (Ap), the best studied LIM-homeodomain transcription factor in Drosophila, cooperates with the cofactor Chip (Chi) to regulate transcription of specific target genes. Although Ap regulates various developmental processes, its function in the adult brain remains unclear. Here, we report that Ap and Chi in the neurons expressing PDF, a neuropeptide, play important roles in proper sleep/wake regulation in adult flies. PDF-expressing neurons consist of two neuronal clusters: small ventral-lateral neurons (s-LNvs) acting as the circadian pacemaker and large ventral-lateral neurons (l-LNvs) regulating light-driven arousal. We identified that Ap localizes to the nuclei of s-LNvs and l-LNvs. In light-dark (LD) cycles, RNAi knockdown or the targeted expression of dominant-negative forms of Ap or Chi in PDF-expressing neurons or l-LNvs promoted arousal. In contrast, in constant darkness, knockdown of Ap in PDF-expressing neurons did not promote arousal, indicating that a reduced Ap function in PDF-expressing neurons promotes light-driven arousal. Furthermore, Ap expression in l-LNvs showed daily rhythms (peaking at midnight), which are generated by a direct light-dependent mechanism rather than by the endogenous clock. These results raise the possibility that the daily oscillation of Ap expression in l-LNvs may contribute to the buffering of light-driven arousal in wild-type flies.

  6. LIMS在药物研发实验室的应用探讨%Discussion on the LIMS Application in Drug R&D Laboratory

    Institute of Scientific and Technical Information of China (English)

    邵彦坤; 廖俊; 陆涛

    2013-01-01

    药物研发是一个多学科相互渗透和相互合作的过程,具有周期长、投入高、风险大的特点.将实验室信息管理系统(Laboratory Information Management System,LIMS)应用于药物研发实验室不仅能持续推进研究进度,降低相关成本,而且能保证实验流程符合相关的国际质量标准和规范.概述了LIMS的发展现状,并对LIMS在药物研发实验室的应用进行了分析,进一步探讨了实际应用中需要解决的几个关键问题,包括LIMS与仪器的集成、物联网技术的应用、生物信息数据库与LIMS的集成.

  7. LIMS引进过程中的问题及对策浅析%Analysis of Problems and Countermeasures in the LIMS Introduction Process

    Institute of Scientific and Technical Information of China (English)

    刘琳娟

    2012-01-01

    简述了实验室信息管理系统的概念,介绍了南通市环境监测中心站在调研实验室信息管理系统过程中的一些体会。着重分析了实验室在考虑工作领域、组织架构、质量目标、体系运行、业务流程、设备配置、人员组成、工作类型和工作量变化等因素的前提下,选择LIMS需遵循的原则、实施前的注意点以及它对实验室在流程和效率方面改善的讨论。%This paper describes the concept of the laboratory information management system, introduces some experience in the process of Nantong Municipal Environmental Monitoring Center research laboratory information management system. Focus analysis laboratory in comidering the areas of work, organizational structure, quality objectives, system operation and business processes, device configuration, composition, under the premise of the type of work and workload changes and other factors, select the LIMS required to follow the principle, prior to the implementation of attention to points as well as its laboratory processes and ettieieney improvement diseussions.

  8. A core transcriptional network composed of Pax2/8, Gata3 and Lim1 regulates key players of pro/mesonephros morphogenesis.

    Science.gov (United States)

    Boualia, Sami Kamel; Gaitan, Yaned; Tremblay, Mathieu; Sharma, Richa; Cardin, Julie; Kania, Artur; Bouchard, Maxime

    2013-10-15

    Translating the developmental program encoded in the genome into cellular and morphogenetic functions requires the deployment of elaborate gene regulatory networks (GRNs). GRNs are especially crucial at the onset of organ development where a few regulatory signals establish the different programs required for tissue organization. In the renal system primordium (the pro/mesonephros), important regulators have been identified but their hierarchical and regulatory organization is still elusive. Here, we have performed a detailed analysis of the GRN underlying mouse pro/mesonephros development. We find that a core regulatory subcircuit composed of Pax2/8, Gata3 and Lim1 turns on a deeper layer of transcriptional regulators while activating effector genes responsible for cell signaling and tissue organization. Among the genes directly affected by the core components are the key developmental molecules Nephronectin (Npnt) and Plac8. Hence, the pro/mesonephros GRN links together several essential genes regulating tissue morphogenesis. This renal GRN sheds new light on the disease group Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) in that gene mutations are expected to generate different phenotypic outcomes as a consequence of regulatory network deficiencies rather than threshold effects from single genes.

  9. The co-factor of LIM domains (CLIM/LDB/NLI) maintains basal mammary epithelial stem cells and promotes breast tumorigenesis.

    Science.gov (United States)

    Salmans, Michael L; Yu, Zhengquan; Watanabe, Kazuhide; Cam, Eric; Sun, Peng; Smyth, Padhraic; Dai, Xing; Andersen, Bogi

    2014-07-01

    Mammary gland branching morphogenesis and ductal homeostasis relies on mammary stem cell function for the maintenance of basal and luminal cell compartments. The mechanisms of transcriptional regulation of the basal cell compartment are currently unknown. We explored these mechanisms in the basal cell compartment and identified the Co-factor of LIM domains (CLIM/LDB/NLI) as a transcriptional regulator that maintains these cells. Clims act within the basal cell compartment to promote branching morphogenesis by maintaining the number and proliferative potential of basal mammary epithelial stem cells. Clim2, in a complex with LMO4, supports mammary stem cells by directly targeting the Fgfr2 promoter in basal cells to increase its expression. Strikingly, Clims also coordinate basal-specific transcriptional programs to preserve luminal cell identity. These basal-derived cues inhibit epidermis-like differentiation of the luminal cell compartment and enhance the expression of luminal cell-specific oncogenes ErbB2 and ErbB3. Consistently, basal-expressed Clims promote the initiation and progression of breast cancer in the MMTV-PyMT tumor model, and the Clim-regulated branching morphogenesis gene network is a prognostic indicator of poor breast cancer outcome in humans.

  10. The expression of LIM-homeobox genes, Lhx1 and Lhx5, in the forebrain is essential for neural retina differentiation.

    Science.gov (United States)

    Inoue, Junji; Ueda, Yuuki; Bando, Tetsuya; Mito, Taro; Noji, Sumihare; Ohuchi, Hideyo

    2013-09-01

    Elucidating the mechanisms underlying eye development is essential for advancing the medical treatment of eye-related disorders. The primordium of the eye is an optic vesicle (OV), which has a dual potential for generation of the developing neural retina and retinal pigment epithelium. However, the factors that regulate the differentiation of the retinal primordium remain unclear. We have previously shown that overexpression of Lhx1 and Lhx5, members of the LIM-homeobox genes, induced the formation of a second neural retina from the presumptive pigmented retina of the OV. However, the precise timing of Lhx1 expression required for neural retina differentiation has not been clarified. Moreover, RNA interference of Lhx5 has not been previously reported. Here, using a modified electroporation method, we show that, Lhx1 expression in the forebrain around stage 8 is required for neural retina formation. In addition, we have succeeded in the knockdown of Lhx5 expression, resulting in conversion of the neural retina region to a pigment vesicle-like tissue, which indicates that Lhx5 is also required for neural retina differentiation, which correlates temporally with the activity of Lhx1. These results suggest that Lhx1 and Lhx5 in the forebrain regulate neural retina differentiation by suppressing the development of the retinal pigment epithelium, before the formation of the OV.

  11. The LIM and POU homeobox genes ttx-3 and unc-86 act as terminal selectors in distinct cholinergic and serotonergic neuron types.

    Science.gov (United States)

    Zhang, Feifan; Bhattacharya, Abhishek; Nelson, Jessica C; Abe, Namiko; Gordon, Patricia; Lloret-Fernandez, Carla; Maicas, Miren; Flames, Nuria; Mann, Richard S; Colón-Ramos, Daniel A; Hobert, Oliver

    2014-01-01

    Transcription factors that drive neuron type-specific terminal differentiation programs in the developing nervous system are often expressed in several distinct neuronal cell types, but to what extent they have similar or distinct activities in individual neuronal cell types is generally not well explored. We investigate this problem using, as a starting point, the C. elegans LIM homeodomain transcription factor ttx-3, which acts as a terminal selector to drive the terminal differentiation program of the cholinergic AIY interneuron class. Using a panel of different terminal differentiation markers, including neurotransmitter synthesizing enzymes, neurotransmitter receptors and neuropeptides, we show that ttx-3 also controls the terminal differentiation program of two additional, distinct neuron types, namely the cholinergic AIA interneurons and the serotonergic NSM neurons. We show that the type of differentiation program that is controlled by ttx-3 in different neuron types is specified by a distinct set of collaborating transcription factors. One of the collaborating transcription factors is the POU homeobox gene unc-86, which collaborates with ttx-3 to determine the identity of the serotonergic NSM neurons. unc-86 in turn operates independently of ttx-3 in the anterior ganglion where it collaborates with the ARID-type transcription factor cfi-1 to determine the cholinergic identity of the IL2 sensory and URA motor neurons. In conclusion, transcription factors operate as terminal selectors in distinct combinations in different neuron types, defining neuron type-specific identity features.

  12. Targeted disruption of the mouse Csrp2 gene encoding the cysteine- and glycine-rich LIM domain protein CRP2 result in subtle alteration of cardiac ultrastructure

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    Stoll Doris

    2008-08-01

    Full Text Available Abstract Background The cysteine and glycine rich protein 2 (CRP2 encoded by the Csrp2 gene is a LIM domain protein expressed in the vascular system, particularly in smooth muscle cells. It exhibits a bimodal subcellular distribution, accumulating at actin-based filaments in the cytosol and in the nucleus. In order to analyze the function of CRP2 in vivo, we disrupted the Csrp2 gene in mice and analysed the resulting phenotype. Results A ~17.3 kbp fragment of the murine Csrp2 gene containing exon 3 through 6 was isolated. Using this construct we confirmed the recently determined chromosomal localization (Chromosome 10, best fit location between markers D10Mit203 proximal and D10Mit150 central. A gene disruption cassette was cloned into exon 4 and a mouse strain lacking functional Csrp2 was generated. Mice lacking CRP2 are viable and fertile and have no obvious deficits in reproduction and survival. However, detailed histological and electron microscopic studies reveal that CRP2-deficient mice have subtle alterations in their cardiac ultrastructure. In these mice, the cardiomyocytes display a slight increase in their thickness, indicating moderate hypertrophy at the cellular level. Although the expression of several intercalated disc-associated proteins such as β-catenin, N-RAP and connexin-43 were not affected in these mice, the distribution of respective proteins was changed within heart tissue. Conclusion We conclude that the lack of CRP2 is associated with alterations in cardiomyocyte thickness and hypertrophy.

  13. Four and a Half LIM Domains 1b (Fhl1b Is Essential for Regulating the Liver versus Pancreas Fate Decision and for β-Cell Regeneration.

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    Jin Xu

    2016-02-01

    Full Text Available The liver and pancreas originate from overlapping embryonic regions, and single-cell lineage tracing in zebrafish has shown that Bone morphogenetic protein 2b (Bmp2b signaling is essential for determining the fate of bipotential hepatopancreatic progenitors towards the liver or pancreas. Despite its pivotal role, the gene regulatory networks functioning downstream of Bmp2b signaling in this process are poorly understood. We have identified four and a half LIM domains 1b (fhl1b, which is primarily expressed in the prospective liver anlage, as a novel target of Bmp2b signaling. fhl1b depletion compromised liver specification and enhanced induction of pancreatic cells from endodermal progenitors. Conversely, overexpression of fhl1b favored liver specification and inhibited induction of pancreatic cells. By single-cell lineage tracing, we showed that fhl1b depletion led lateral endodermal cells, destined to become liver cells, to become pancreatic cells. Reversely, when fhl1b was overexpressed, medially located endodermal cells, fated to differentiate into pancreatic and intestinal cells, contributed to the liver by directly or indirectly modulating the discrete levels of pdx1 expression in endodermal progenitors. Moreover, loss of fhl1b increased the regenerative capacity of β-cells by increasing pdx1 and neurod expression in the hepatopancreatic ductal system. Altogether, these data reveal novel and critical functions of Fhl1b in the hepatic versus pancreatic fate decision and in β-cell regeneration.

  14. Capacidad de uso de las tierras en la microcuenca El Limón, complejo de cuencas Sabana Yegua, República Dominicana Land use capacity in the mini watershed El Limón, Sabana Yegua Watershed Complex, Dominican Republic

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    Maldané Cuello Espinoza

    2013-03-01

    Full Text Available Este estudio aborda el análisis biofísico de las tierras en la microcuenca El Limón, en el complejo de Cuencas Altas de Sabana Yegua en República Dominicana. El objetivo del ensayo fue determinar la capacidad de uso de las tierras considerando las limitaciones del suelo, el clima y la geomorfología que presenta. Se propone una serie de prácticas de manejo como opciones para reducir la erosión, mejorar el suelo y acondicionar los caminos, lo que permitiría mantener condiciones favorables para el manejo racional del sistema. De esa forma, con este objetivo se establecen y se integran por primera vez en una cuenca del país varios elementos limitantes para con su identificación proponer prácticas apropiadas que controlen el problema de degradación acelerada que experimenta el área bajo estudio. Para ello, se determinaron parámetros fundamentales, los cuales abarcan factores de erosión, suelo, drenaje y clima, en función de lo que establece la metodología MAG/FAO/UNED (1996, la cual se usa en Costa Rica para determinar la capacidad de uso de las tierras y ha sido adaptada al área objeto de este estudio. Esta metodología integra clases de tierras que van desde la I hasta la VIII según la frecuencia y grado de las limitantes que se identifican. Para su aplicación en la microcuenca El Limón, se han incluido otros parámetros físicos que no contemplan dicha metodología, tales como la densidad aparente del suelo y los parámetros climáticos de lluvia y temperatura, estos últimos con el fin de establecer la zona de vida del área. Se pudo determinar que las clases de tierras en la microcuenca El Limón van desde la III hasta la VIII, las cuales son aptas para protección, sistemas forestales y agroforestería. De igual manera, se estableció que las mayores limitantes que se presentan son las concernientes a la erosión y al drenaje del suelo. Para frenar y corregir los efectos de estas limitantes, las prácticas de manejo

  15. "Erlösung ist, wenn man schulfrei hat!". Erste Ergebnisse einer qualitativ-empirischen Studie zur Erfassung und didaktischen Bearbeitung von Erlösungsvorstellungen 10- bis 14-jähriger SchülerInnen der Sekundarstufe I in Österreich.

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    Thomas Weiß

    2014-07-01

    Full Text Available ENGLISH: The teaching of redemption of mankind through God in Jesus Christ is fundamental to the Christian religion. This article expands on this central topic and presents the first results of a research carried out in schools as part of a current qualitative study about what ideas of redemption ten- to fourteen-year-old students have. The aim of this basic empirical research is to generate views and perspectives on ‚redemption‘ in children and adolescents. A further objective is to develop and integrate didactic tasks for Religious Education as a subject, in order to work on the topic of ‚redemption‘. By doing so, theological reflection should be applied and an alignment within the spheres of life of young people should be provided in lessons at School. DEUTSCH: Die Rede von der Erlösung des Menschen durch Gott in Jesus Christus ist für die christliche Religion fundamental. Der Beitrag widmet sich diesem zentralen Thema und präsentiert die ersten Forschungsergebnisse, die im Rahmen einer aktuellen qualitativen Studie zu Erlösungsvorstellungen von 10- bis 14-jährigen SchülerInnen in Schulen erhoben wurde. Diese grundlagenorientierte empirische Forschungsarbeit hat zum Ziel, kindliche / jugendliche Denk- und Sichtweisen zu ‚Erlösung’ zu generieren und didaktische Aufgaben im Religionsunterricht zu entwickeln und zu implementieren, um das Thema ‚Erlösung’ lebensweltlich orientiert und theologisch reflektiert im schulischen Unterricht bearbeiten zu können.

  16. Densidades de plantio e doses de biofertilizante na produção de capim-limão Density and biofertilizer levels for lemon grass production

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    Arie F Blank

    2007-09-01

    Full Text Available O objetivo do trabalho foi avaliar o efeito de densidades de plantio e doses de biofertilizante comercial na produção de capim-limão (Cymbopogon citratus (D.C. Stapf. O experimento foi implantado em parcelas subdivididas com três repetições, colocando-se nas parcelas as densidades de plantio (33.333, 49.382, 55.555 e 111.111 plantas ha-1 e nas subparcelas as doses de biofertilizante (0; 20; 40 e 60 t ha-1. Foram realizadas nove colheitas em intervalos de 42 dias. Avaliou-se a sobrevivência, a altura de planta, o número de perfilhos por touceira, a massa seca por touceira e da parte aérea, o teor e o rendimento de óleo essencial. O teor de óleo essencial não foi influenciado pelas densidades de plantio e doses de biofertilizante. A densidade crescente resultou em regressões lineares crescentes para o rendimento de óleo essencial e a massa seca da parte aérea e em uma regressão linear decrescente para a massa seca por touceira. A dose de biofertilizante resultou em regressões lineares crescentes para o rendimento de óleo essencial e a massa seca por touceira e da parte aérea. A densidade de 111.111 plantas ha-1 (0,30 x 0,30 m e a dose de biofertilizante de 60 t ha-1 no primeiro ano de cultivo poderão ser recomendados para produção do capim-limão.The aim of this work was to evaluate the plant density and biofertilizer levels on lemon grass (Cymbopogom citratus (D.C. Stapf. The experiment was installed in split plot design with three replications, having plant density as plots (33,333; 49,382; 55,555 and 111,111 plants ha-1 and biofertilizer doses as sub plots (0; 20; 40; 60 t ha-1 year-1. Nine cuttings were realized using an interval of 42 days between cuttings. We evaluated survival, plant height, number of tillers per plant, dry weight per plant and of biomass yield, essential oil content and yield. The essential oil content was not influenced by the plant densities and biofertilizer doses. The crescent density resulted in

  17. LIM homeobox transcription factor Lhx2 inhibits skeletal muscle differentiation in part via transcriptional activation of Msx1 and Msx2.

    Science.gov (United States)

    Kodaka, Yusaku; Tanaka, Kiyoko; Kitajima, Kenji; Tanegashima, Kosuke; Matsuda, Ryoichi; Hara, Takahiko

    2015-02-15

    LIM homeobox transcription factor Lhx2 is known to be an important regulator of neuronal development, homeostasis of hair follicle stem cells, and self-renewal of hematopoietic stem cells; however, its function in skeletal muscle development is poorly understood. In this study, we found that overexpression of Lhx2 completely inhibits the myotube-forming capacity of C2C12 cells and primary myoblasts. The muscle dedifferentiation factors Msx1 and Msx2 were strongly induced by the Lhx2 overexpression. Short interfering RNA-mediated knockdown of Lhx2 in the developing limb buds of mouse embryos resulted in a reduction in Msx1 and Msx2 mRNA levels, suggesting that they are downstream target genes of Lhx2. We found two Lhx2 consensus-binding sites in the -2097 to -1189 genomic region of Msx1 and two additional sites in the -536 to +73 genomic region of Msx2. These sequences were shown by luciferase reporter assay to be essential for Lhx2-mediated transcriptional activation. Moreover, electrophoretic mobility shift assays and chromatin immunoprecipitation assays showed that Lhx2 is present in chromatin DNA complexes bound to the enhancer regions of the Msx1 and Msx2 genes. These data demonstrate that Msx1 and Msx2 are direct transcriptional targets of Lhx2. In addition, overexpression of Lhx2 significantly enhanced the mRNA levels of bone morphogenetic protein 4 and transforming growth factor beta family genes. We propose that Lhx2 is involved in the early stage of skeletal muscle development by inducing multiple differentiation inhibitory factors.

  18. Four and a half LIM protein 1C (FHL1C: a binding partner for voltage-gated potassium channel K(v1.5.

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    Ivana Poparic

    Full Text Available Four-and-a-half LIM domain protein 1 isoform A (FHL1A is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA. We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0/G(1 phase. Furthermore, low expression of K(v1.5, a voltage-gated potassium channel known to alter myoblast proliferation during the G(1 phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5 and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5 within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5 with FHL1C in Xenopus laevis oocytes markedly reduced K(+ currents when compared to oocytes expressing K(v1.5 only. We here present the first evidence on a biological relevance of FHL1C.

  19. [Intestinal parasites in white-faced capuchin monkeys Cebus capucinus (Primates: Cebidae) inhabiting a protected area in the Limón province of Northeastern Costa Rica].

    Science.gov (United States)

    Chinchilla, Misael; Urbani, Bernardo; Valerio, Idalia; Vanegas, Juan Carlos

    2010-12-01

    Deforestation of tropical forests is threatening monkey biodiversity and their health status, dependent of an ecologically undisturbed area. To asses this relationship, we analyzed parasite occurrence in their intestines. The study was conducted at the Estación Biológica La Suerte (EBLS), Limón, Costa Rica. The group of white-faced capuchin monkeys (Cebus capucinus) was observed between March and December of 2006. A total of 75 feces samples were obtained. Once a sample was collected, the eaten plant type was identified to family and species level, and feces were processed in the laboratory to determine parasite incidence. Results showed that Moraceae was the most represented family in the samples. Among parasites, Strongyloides spp. and Acanthocephala were the most common. Positive prevalence of parasites was found similar and independent of sex and age of capuchin individuals. Microsporids were mainly reported in feces associated with Piperaceae. A low presence of these parasites was found in samples associated with Myrtaceae, with possible anti-parasite active components. The occurrence of parasites was relatively high in EBLS, when compared to other regions in Costa Rica. The higher occurrence of parasites observed in capuchins at EBLS may be due to the fact that this rain forest is surrounded by areas affected by human activities. We suggest the promotion of research in neotropical primates parasitology, for a better comprehension of the parasite-host relationship, and in a long term, being able to understand the ecosystems where they coexist, and consequently, preserve the biodiversity of the whole region.

  20. Parasitosis intestinal en monos capuchinos cariblancos Cebus capucinus (Primates: Cebidae de un área protegida en la provincia de Limón, noreste de Costa Rica

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    Misael Chinchilla

    2010-12-01

    Full Text Available La deforestación de bosques tropicales está amenazando la biodiversidad de las especies de primates y su estado de salud, dependiente de un lugar ecológicamente equilibrado. Con el fin de evaluar esta relación, se analizó la presencia de parásitos en los intestinos de esta especie. El estudio se realizó en la Estación Biológica La Suerte (EBLS, Limón, Costa Rica. El grupo de monos capuchinos cariblancos (Cebus capucinus fue observado entre marzo y diciembre de 2006. Se recolectaron 75 muestras de heces a las que se les identificó las plantas ingeridas, y se procesaron en el laboratorio para evaluar la prevalencia de parásitos. Moraceae fue la familia de plantas más reportada. Strongyloides spp. y acantocéfalos fueron los más comunes. La distribución positiva de las mismas fue similar independientemente del sexo y la edad. Microsporidios se reportaron mayoritariamente en heces asociadas con la familia Piperaceae. Fue encontrada una baja existencia de éstos parásitos en muestras asociadas con Myrtaceae en cuyo género Psidium, se han reportado compuestos activos antiparasitarios. La aparición de parásitos fue relativamente mayor en la EBLS, comparado con muestras de otras regiones más secas de Costa Rica. Por lo tanto, esa expresión parasitaria puede deberse al hecho de ser la EBLS una selva lluviosa, además de estar rodeada por zonas con actividades antrópicas. Se sugiere incrementar las investigaciones de parasitología de campo en primates neotropicales con el fin de entender plenamente las relaciones parásitos-hospederos, para en un largo plazo poder comprender los ecosistemas donde conviven, y en consecuencia, preservar la diversidad biológica.

  1. The LIM homeodomain transcription factor LHX6: a transcriptional repressor that interacts with pituitary homeobox 2 (PITX2) to regulate odontogenesis.

    Science.gov (United States)

    Zhang, Zichao; Gutierrez, Diana; Li, Xiao; Bidlack, Felicitas; Cao, Huojun; Wang, Jianbo; Andrade, Kelsey; Margolis, Henry C; Amendt, Brad A

    2013-01-25

    LHX6 is a LIM-homeobox transcription factor expressed during embryogenesis; however, the molecular mechanisms regulating LHX6 transcriptional activities are unknown. LHX6 and the PITX2 homeodomain transcription factor have overlapping expression patterns during tooth and craniofacial development, and in this report, we demonstrate new transcriptional mechanisms for these factors. PITX2 and LHX6 are co-expressed in the oral and dental epithelium and epithelial cell lines. Lhx6 expression is increased in Pitx2c transgenic mice and decreased in Pitx2 null mice. PITX2 activates endogenous Lhx6 expression and the Lhx6 promoter, whereas LHX6 represses its promoter activity. Chromatin immunoprecipitation experiments reveal endogenous PITX2 binding to the Lhx6 promoter. LHX6 directly interacts with PITX2 to inhibit PITX2 transcriptional activities and activation of multiple promoters. Bimolecular fluorescence complementation assays reveal an LHX6·PITX2 nuclear interaction in living cells. LHX6 has a dominant repressive effect on the PITX2 synergistic activation with LEF-1 and β-catenin co-factors. Thus, LHX6 acts as a transcriptional repressor and represses the expression of several genes involved in odontogenesis. We have identified specific defects in incisor, molar, mandible, bone, and root development and late stage enamel formation in Lhx6 null mice. Amelogenin and ameloblastin expression is reduced and/or delayed in the Lhx6 null mice, potentially resulting from defects in dentin deposition and ameloblast differentiation. Our results demonstrate that LHX6 regulates cell proliferation in the cervical loop and promotes cell differentiation in the anterior region of the incisor. We demonstrate new molecular mechanisms for LHX6 and an interaction with PITX2 for normal craniofacial and tooth development.

  2. Nuclear localization of lymphocyte-specific protein tyrosine kinase (Lck) and its role in regulating LIM domain only 2 (Lmo2) gene

    Energy Technology Data Exchange (ETDEWEB)

    Venkitachalam, Srividya; Chueh, Fu-Yu [Department of Microbiology and Immunology, H. M. Bligh Cancer Research Laboratories, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064 (United States); Yu, Chao-Lan, E-mail: chaolan.yu@rosalindfranklin.edu [Department of Microbiology and Immunology, H. M. Bligh Cancer Research Laboratories, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064 (United States)

    2012-01-20

    Highlights: Black-Right-Pointing-Pointer Lmo2 expression is elevated in Lck-transformed cells. Black-Right-Pointing-Pointer Both endogenous and exogenous Lck localize in the nucleus. Black-Right-Pointing-Pointer Nuclear Lck is active in Lck-transformed cells. Black-Right-Pointing-Pointer Lck binds to the promoter region of Lmo2 gene in vivo. Black-Right-Pointing-Pointer In contrast to JAK2, Lck does not increase histone H3 phosphorylation on Tyr 41. -- Abstract: LIM domain only protein 2 (Lmo2) is a transcription factor that plays a critical role in the development of T-acute lymphoblastic leukemia (T-ALL). A previous report established a link between Lmo2 expression and the nuclear presence of oncogenic Janus kinase 2 (JAK2), a non-receptor protein tyrosine kinase. The oncogenic JAK2 kinase phosphorylates histone H3 on Tyr 41 that leads to the relief of Lmo2 promoter repression and subsequent gene expression. Similar to JAK2, constitutive activation of lymphocyte-specific protein tyrosine kinase (Lck) has been implicated in lymphoid malignancies. However, it is not known whether oncogenic Lck regulates Lmo2 expression through a similar mechanism. We show here that Lmo2 expression is significantly elevated in T cell leukemia LSTRA overexpressing active Lck kinase and in HEK 293 cells expressing oncogenic Y505FLck kinase. Nuclear localization of active Lck kinase was confirmed in both Lck-transformed cells by subcellular fractionation and immunofluorescence microscopy. More importantly, in contrast to oncogenic JAK2, oncogenic Lck kinase does not result in significant increase in histone H3 phosphorylation on Tyr 41. Instead, chromatin immunoprecipitation experiment shows that oncogenic Y505FLck kinase binds to the Lmo2 promoter in vivo. This result raises the possibility that oncogenic Lck may activate Lmo2 promoter through direct interaction.

  3. Olfactory and amygdalar structures of the chicken ventral pallium based on the combinatorial expression patterns of LIM and other developmental regulatory genes.

    Science.gov (United States)

    Abellán, Antonio; Legaz, Isabel; Vernier, Baptiste; Rétaux, Sylvie; Medina, Loreta

    2009-09-20

    We compared the combinatorial expression patterns of several LIM domain-containing regulatory genes in the ventrolateral pallium of mouse and chicken, in order to identify the homologues of the ventral pallial amygdala and other olfactory structures in birds. Lmo3, Lmo4, Lhx2, and Lhx9 showed comparable expression patterns in the telencephalon of mouse and chicken, which allowed distinction of the ventrolateral pallium and, particularly, the ventral pallial amygdala and entorhinal cortex. Lmo3 was expressed in most of the ventrolateral pallium in both species, including, in chicken, the piriform cortex and dorsal ventricular ridge (mesopallium, nidopallium, and arcopallium) and, in mouse, the piriform cortex, most of the claustral complex, and the pallial amygdala. Lhx9 was differentially expressed in the ventral pallium, where it was restricted to its rostral (olfactory bulb) and caudal (amygdalar and entorhinal) poles. In the caudal pole, expression of Lhx9 overlapped that of its paralog Lhx2. According to these expression patterns, the chicken ventral pallial amygdala appears to include the caudal dorsolateral pallium, the caudal nidopallium, and the whole arcopallium, and each one relates to a distinct ventricular sector. Finally, the combinatorial expression patterns of Lmo3, Lhx9, and Lmo4 distinguished four distinct subdivisions in the superficial, olfactorecipient area of the chicken ventral pallium, which appear comparable to the piriform, entorhinal, amygdalopiriform, and amygdalar cortices of mammals. The results are discussed in the context of the two existing, opposite views on the homology of the dorsal ventricular ridge of sauropsids and in terms of the evolution of pallial derivatives.

  4. Avaliação genética de seleções e híbridos de limões cravo, volkameriano e rugoso como porta-enxertos para laranjeiras Valência na presença da morte súbita dos citros

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    Jorgino Pompeu Junior

    2013-03-01

    Full Text Available Este estudo teve como objetivo realizar a avaliação genética da produção de frutos, eficiência produtiva e altura de laranjeiras Valência (Citrus sinensis enxertadas em seleções e híbridos dos limões Cravo (C. limonia, Volkameriano (C. volkameriana e Rugoso (C. jambhiri, em área endêmica para morte súbita dos citros (MSC. Foram avaliados 36 genótipos desses porta-enxertos, representados por cinco plantas cada, avaliados em cinco safras, do terceiro ao sétimo ano após o plantio. Sete dos genótipos avaliados apresentaram plantas com sintomas de MSC até o sétimo ano: Rangpur Otaheite orange 12901 (859, Rangpur Red Lime D.33.30 (866, Limão-Cravo EEL (871, Rangpur Borneo red (874, Citrus kokhai (1649, Limão-Rugoso 58329 (1655 e Limão- Cravo x Swingle B (1695. Para os genótipos que não manifestaram sintomas da doença, foram estimados parâmetros genéticos e fenotípicos e realizada a predição de valores genéticos dos indivíduos, visando à seleção e ao melhoramento genético para as características citadas, empregando-se o método REML/BLUP (máxima verossimilhança restrita/melhor predição linear não viciada. A análise de produção de frutos de cinco safras mostrou acurácia seletiva de 84,59%, tornando-se desnecessária a avaliação de maior número de safras. A seleção dos sete melhores genótipos proporcionou ganhos genéticos de 11,5% na produção de frutos, enquanto a do melhor genótipo conferiu ganho genético de 16,3%. As maiores médias genéticas preditas (>70,0 kg.pl-1 para produção de frutos foram obtidas pelos genótipos Limão-Cravo- Ipanema (1522, Santa- Bárbara-Red- Lime (884, Limão- Cravo- Limeira (863, Limão- Cravo- Taquaritinga (869, Limão- Rugoso- do -Cabo (1643, Rangpur- Rose Lime (868 e Limão- Cravo- da- Califórnia (1467. Já a acurácia seletiva da eficiência produtiva, para quatro colheitas, foi 77,4%. Para este caráter, as maiores médias genéticas (>8,0 kg.m-3 foram dos

  5. The LIM domain protein FHL2 interacts with the NR5A family of nuclear receptors and CREB to activate the inhibin-α subunit gene in ovarian granulosa cells.

    Science.gov (United States)

    Matulis, Christina K; Mayo, Kelly E

    2012-08-01

    Nuclear receptor transcriptional activity is enhanced by interaction with coactivators. The highly related nuclear receptor 5A (NR5A) subfamily members liver receptor homolog 1 and steroidogenic factor 1 bind to and activate several of the same genes, many of which are important for reproductive function. To better understand transcriptional activation by these nuclear receptors, we sought to identify interacting proteins that might function as coactivators. The LIM domain protein four and a half LIM domain 2 (FHL2) was identified as interacting with the NR5A receptors in a yeast two-hybrid screen of a human ovary cDNA library. FHL2, and the closely related FHL1, are both expressed in the rodent ovary and in granulosa cells. Small interfering RNA-mediated knockdown of FHL1 and FHL2 in primary mouse granulosa cells reduced expression of the NR5A target genes encoding inhibin-α and P450scc. In vitro assays confirmed the interaction between the FHL and NR5A proteins and revealed that a single LIM domain of FHL2 is sufficient for this interaction, whereas determinants in both the ligand binding domain and DNA binding domain of NR5A proteins are important. FHL2 enhances the ability of both liver receptor homolog 1 and steroidogenic factor 1 to activate the inhibin-α subunit gene promoter in granulosa cells and thus functions as a transcriptional coactivator. FHL2 also interacts with cAMP response element-binding protein and substantially augments activation of inhibin gene expression by the combination of NR5A receptors and forskolin, suggesting that FHL2 may facilitate integration of these two signals. Collectively these results identify FHL2 as a novel coactivator of NR5A nuclear receptors in ovarian granulosa cells and suggest its involvement in regulating target genes important for mammalian reproduction.

  6. Caracterização fenotípica, fitoquímica e molecular de populações de Elionurus sp. Humb. & Bompl ex Willd (capim-limão)

    OpenAIRE

    Thanise Nogueira Füller

    2008-01-01

    O capim-limão é uma planta herbácea, perene, que ocorre naturalmente no estado do Rio Grande do Sul, Brasil. Seu potencial econômico se deve ao fato de apresentar óleo essencial utilizado como aromatizante e flavorizante, além de apresentar atividade bactericida atribuída tanto ao óleo quanto ao conteúdo de compostos fenólicos. Apesar da importância, poucos estudos foram desenvolvidos para esta espécie. Sendo assim, o presente trabalho teve como objetivo analisar populações nativas de Elionur...

  7. Aplicación de modelos matemáticos al proceso de secado asistido por infrarrojos de descartes de limón (citrus limon (l.) burm. f. cv. genova)

    OpenAIRE

    PUENTE-DÍAZ, LUIS; ECHEGARAY- PACHECO, ERIC; CASTRO- MONTERO, EDUARDO; Di Scala,Karina

    2013-01-01

    Los desechos agroindustriales son uno de los principales problemas ambientales a nivel mundial. Debido a las altas exigencias de calidad, la comercialización de limones de exportación genera grandes cantidades de desechos. Los descartes de limón contienen fi bra, pectina, potasio, calcio y vitamina C. Por lo tanto, es importante desarrollar alternativas de procesamiento de estos sub-productos para agregarles valor y aumentar su vida útil. El objetivo de la presente investigación fue la aplica...

  8. Aplicación de modelos matemáticos al proceso de secado asistido por infrarrojos de descartes de limón (Citrus limon (L.) Burm. F. Cv. Genova)

    OpenAIRE

    Luis Puente-Díaz; Eric Echegaray-Pacheco; Eduardo Castro- Montero; Karina Di Scala

    2013-01-01

    Los desechos agroindustriales son uno de los principales problemas ambientales a nivel mundial. Debido a las altas exigencias de calidad, la comercialización de limones de exportación genera grandes cantidades de desechos. Los descartes de limón contienen fibra, pectina, potasio, calcio y vitamina C. Por lo tanto, es importante desarrollar alternativas de procesamiento de estos sub-productos para agregarles valor y aumentar su vida útil. El objetivo de la presente investigación fue la aplicac...

  9. Preferência de saúva limão, Atta sexdens rubropilosa Forel, 1908 (Hymenoptera, formicidae a diferentes espécies florestais, em condições de laboratório.

    Directory of Open Access Journals (Sweden)

    Otávio Peres Filho

    2010-08-01

    Full Text Available Avaliou-se a preferência de saúva limão, Atta sexdens rubropilosa Forel, 1908, para 41 espécies florestais nativas e exóticas, em condições de laboratório. As espécies mais transportadas foram gmelina (Gmelina arborea, leucena (Leucaena leucocephala, pau-ferro (Caesalpinia ferrea, albizia (Albizia lebbeck e orelha-de-negro (Enterolobium contortisiliquum e as menos transportadas foram eucalipto (Eucalyptus grandis, jequitibá (Cariniana strellensis e mutamba (Guazuma tomentosa.

  10. Determinación del tamaño comercial de la ostra de manglar, Crassostrea rhizophorae (Guilding, 1828) en sistema de cultivo suspendido en Estero Viscaya, Limón, Costa Rica

    OpenAIRE

    Cabrera Peña, Jorge; Zamora Madriz, Eduardo; Pacheco Urpí, Oscar

    2016-01-01

    En un estudio piloto entre enero y junio de 1982, en ostra de manglar (Crassostrea rhizophorae) cultivada en sistema suspendido, en el Estero Vizcaya, Limón, Costa Atlántica de Costa Rica, se encontró que los índices de condición alcanzan sus más altos porcentajes a la edad de cuatro meses (para peso seco) y cinco meses (para peso húmedo). Se halló una alta correlación entre el peso carne y valvas tanto húmedo como seco, peso del fluído intervalvar y rendimiento con la longitud total; se calc...

  11. “Competitividad de la Agroindustria de Exportación de Lima y Limón: Caso Comparado de Michoacán – Colima, en México y Murcia, en España”

    OpenAIRE

    2012-01-01

    La competitividad permite identificar las claves del desarrollo económico de sectores específicos, incluso de los países. Los nuevos retos competitivos exigen tener sectores claves y en crecimiento para lograr posicionarse en el comercio internacional. La finalidad de la presente investigación es contribuir a la formulación de estrategias para mejorar la competitividad de las empresas exportadoras de la cadena agroalimentaria de lima y limón en México. Para ello se plantea como...

  12. Assessment of the sea-ice carbon pump: Insights from a three-dimensional ocean-sea-ice biogeochemical model (NEMO-LIM-PISCES

    Directory of Open Access Journals (Sweden)

    Sébastien Moreau

    2016-08-01

    Full Text Available Abstract The role of sea ice in the carbon cycle is minimally represented in current Earth System Models (ESMs. Among potentially important flaws, mentioned by several authors and generally overlooked during ESM design, is the link between sea-ice growth and melt and oceanic dissolved inorganic carbon (DIC and total alkalinity (TA. Here we investigate whether this link is indeed an important feature of the marine carbon cycle misrepresented in ESMs. We use an ocean general circulation model (NEMO-LIM-PISCES with sea-ice and marine carbon cycle components, forced by atmospheric reanalyses, adding a first-order representation of DIC and TA storage and release in/from sea ice. Our results suggest that DIC rejection during sea-ice growth releases several hundred Tg C yr−1 to the surface ocean, of which < 2% is exported to depth, leading to a notable but weak redistribution of DIC towards deep polar basins. Active carbon processes (mainly CaCO3 precipitation but also ice-atmosphere CO2 fluxes and net community production increasing the TA/DIC ratio in sea-ice modified ocean-atmosphere CO2 fluxes by a few Tg C yr−1 in the sea-ice zone, with specific hemispheric effects: DIC content of the Arctic basin decreased but DIC content of the Southern Ocean increased. For the global ocean, DIC content increased by 4 Tg C yr−1 or 2 Pg C after 500 years of model run. The simulated numbers are generally small compared to the present-day global ocean annual CO2 sink (2.6 ± 0.5 Pg C yr−1. However, sea-ice carbon processes seem important at regional scales as they act significantly on DIC redistribution within and outside polar basins. The efficiency of carbon export to depth depends on the representation of surface-subsurface exchanges and their relationship with sea ice, and could differ substantially if a higher resolution or different ocean model were used.

  13. Aplicación de modelos matemáticos al proceso de secado asistido por infrarrojos de descartes de limón (Citrus limon (L. Burm. F. Cv. Genova

    Directory of Open Access Journals (Sweden)

    Luis Puente-Díaz

    2013-01-01

    Full Text Available Los desechos agroindustriales son uno de los principales problemas ambientales a nivel mundial. Debido a las altas exigencias de calidad, la comercialización de limones de exportación genera grandes cantidades de desechos. Los descartes de limón contienen fibra, pectina, potasio, calcio y vitamina C. Por lo tanto, es importante desarrollar alternativas de procesamiento de estos sub-productos para agregarles valor y aumentar su vida útil. El objetivo de la presente investigación fue la aplicación del secado asistido por infrarrojos, como tecnología emergente, para estudiar el efecto de la temperatura de proceso sobre las cinéticas de secado de descartes de limón a 30, 40 y 50°C. Se utilizaron seis modelos matemáticos de capa fina encontrados en literatura para representar la cinética experimental. Basándose en análisis estadístico, el modelo Logarítmico presentó el mejor ajuste. Los valores de la difusividad efectiva del agua se estimaron en el rango de 9.45× 10-10 – 3.71× 10-9 m/s2. El valor de la energía de activación fue 55.90 kJ/mol.

  14. Morte súbita dos citros: suscetibilidade de seleções de limão-cravo e uso de interenxertos Citrus sudden death: susceptibility of rangpur lime selections and the use of interstocks

    Directory of Open Access Journals (Sweden)

    Jorgino Pompeu Junior

    2008-12-01

    Full Text Available A morte súbita dos citros é uma doença que afeta cultivares de laranjas e tangerinas enxertadas nos limões-Cravo e Volkameriano. Ela foi observada em plantas com dois a seis anos de idade que, após mostrarem sintomas gerais de declínio, entraram em colapso e morreram. A retirada da casca dos porta-enxertos suscetíveis revela o amarelecimento na região cambial, sendo esse o sintoma-diagnóstico da doença e que precede os sintomas da copa. As plantas enxertadas nas tangerinas Cleópatra e Sunki, no trifoliata e no citrumelo Swingle, não mostram sintomas da doença. A transmissão por borbulha e a evolução espacial sugerem que a morte súbita dos citros seja causada por patógeno transmitido por vetor alado. Com o objetivo de selecionar porta-enxertos tolerantes à doença, laranjeiras Valência enxertadas em 254 porta-enxertos foram plantadas em maio de 2003 e 2004 em solos onde foram erradicados pomares afetados pela morte súbita dos citros e próximos a pomares afetados pela doença. Em novembro de 2006, o sintoma-diagnóstico da doença estava presente em dez seleções de limão-Cravo: Santa Barbara red lime, Borneo red lime, Limão-Cravo Taquaritinga, Rangpur India C-26-1, Rangpur rose lemon, Rangpur Kusaie lime, Rangpur red lime D-33-40, Rangpur Egyptian lime, Rangpur lemon India e Japanshe citroen. A presença de interenxerto de trifoliata ou de tangerina Cleópatra, entre o limão-Cravo e a laranja Valência, não impediu a manifestação da doença.Citrus sudden death (CSD or morte súbita dos citros affects sweet orange cultivars and some mandarin trees grafted on Rangpur lime and Volkamer lemon rootstocks. The disease was observed in trees with ages ranging from two to six years; after showing general decline symptoms, the affected trees suddenly collapse and die. Trees on Cleopatra and Sunki mandarins, 'Swingle' citrumelo and trifoliate orange showed no symptoms of CSD. Cambial yellowing in the rootstock can be observed

  15. Infants' Preferential Attention to Sung and Spoken Stimuli

    Science.gov (United States)

    Costa-Giomi, Eugenia; Ilari, Beatriz

    2014-01-01

    Caregivers and early childhood teachers all over the world use singing and speech to elicit and maintain infants' attention. Research comparing infants' preferential attention to music and speech is inconclusive regarding their responses to these two types of auditory stimuli, with one study showing a music bias and another one…

  16. Sung Solecisms: Hip Hop as Non-Prescriptive Pedagogy

    Science.gov (United States)

    Biggs, Cynthia D.

    2011-01-01

    Does the music idiom commonly known as rap music have educational merit? With its harshest critics lambasting it with connotations that equate it with stupidity, gun culture, jail culture, or non-intellectual culture, could there be a pedagogical link after all? Dr. Marc Lamont Hill, Associate Professor of English Education and Anthropology at…

  17. "The word was sung, the word was bound" / Lester Capon

    Index Scriptorium Estoniae

    Capon, Lester, 1951-

    2010-01-01

    4. rahvusvaheline köitekunsti näitus-konkurss "Scripta manent" Eesti Tarbekunsti- ja Disainimuuseumis 9. jaanuarini 2011. Köita tuli Veljo Tormise "Lauldud sõna". Jeff Clementsi, Katinka Keusi (Holland), Lolita Grabauskiene-Tarbunaite (Leedu), Tiina Piisangu, Maila Käose, Ruuda Maarandi töödest

  18. Bowhead whale songs sung by females in Disko Bay, Greenland

    DEFF Research Database (Denmark)

    Tervo, Outi; Christoffersen, Mads; FØrasier, Timothy;

    2011-01-01

    for this species. Bowhead whale song has long been hypothesized to be produced by male whales as a reproductive advertisement; however, no data on the sex of singers has ever been reported. In this study, we determine the sex of singing bowhead whales Balaena mysticetus in Disko Bay, West Greenland, by localizing...... of female bowhead whales may function for mate attraction and represent a novel example of partial courtship role reversal in mammals....

  19. Entweder/Und, Wiederkunft/Erlösung

    OpenAIRE

    Löschenkohl, Birte

    2016-01-01

    Mit merklicher Begeisterung verfasst Walter Benjamin am 6. Januar 1938 einen Brief an Max Horkheimer, in dem er ihm von einem "seltnen Fund" von höchster Bedeutsamkeit berichtet, der seine Arbeit "entscheidend beeinflussen" werde (GB VI, 9). Bei dem Fund handelte es sich um Louis Auguste Blanquis (1805–1881) kosmologische Schrift 'L’Éternité par les astres', verfasst 1871 im Kerker einer Gefängnisburg vor der Küste der Bretagne. Der Autor, einer der umtriebigsten Revolutionäre Frankreichs im ...

  20. "The word was sung, the word was bound" / Lester Capon

    Index Scriptorium Estoniae

    Capon, Lester, 1951-

    2010-01-01

    4. rahvusvaheline köitekunsti näitus-konkurss "Scripta manent" Eesti Tarbekunsti- ja Disainimuuseumis 9. jaanuarini 2011. Köita tuli Veljo Tormise "Lauldud sõna". Jeff Clementsi, Katinka Keusi (Holland), Lolita Grabauskiene-Tarbunaite (Leedu), Tiina Piisangu, Maila Käose, Ruuda Maarandi töödest

  1. Bowhead whale songs sung by females in Disko Bay, Greenland

    DEFF Research Database (Denmark)

    Tervo, Outi; Christoffersen, Mads; FØrasier, Timothy

    2011-01-01

    Song is commonly associated with breeding behaviour with singing primarily occurring during the breeding season and in most species, singers are male. Bowhead whales produce complex song, primarily during the late fall through spring, a period believed to correspond to the mating season for this ......Song is commonly associated with breeding behaviour with singing primarily occurring during the breeding season and in most species, singers are male. Bowhead whales produce complex song, primarily during the late fall through spring, a period believed to correspond to the mating season...... for this species. Bowhead whale song has long been hypothesized to be produced by male whales as a reproductive advertisement; however, no data on the sex of singers has ever been reported. In this study, we determine the sex of singing bowhead whales Balaena mysticetus in Disko Bay, West Greenland, by localizing......% males (n=1)). These data clearly demonstrate that female bowhead whales sing, however more samples are necessary to assess whether male bowhead whales also sing. The suggested functions of female song for other species include territorial defense, mate guarding, coordination of breeding activities...

  2. Changes in the structure and pigmentation of the eyes of honeybee (Apis mellifera L. queens with the "limão" mutation

    Directory of Open Access Journals (Sweden)

    José Chaud-Netto

    2000-03-01

    Full Text Available This study describes the ultrastructural differences between the compound eyes of ch li/ch li and Ch/ch li honeybee queens. Heterozygous "limão" bees had an almost normal ultrastructural organization of the ommatidia, but there were some alterations, including small vacuoles in the crystalline cones and a loss of pigment by primary pigmentary cells. In homozygous bees many ommatidia had very deformed crystalline cones and there were some bipartite rhabdoma. There was a reduction in the amount of pigment in the primary and secondary pigmentary cells and receptor cells (retinulae of mutant eyes. However, the eyes of both heterozygous and homozygous queens had the same type of pigment granules. Certain membrane-limited structures containing pigment granules and electron-dense material appeared to be of lysosomal nature. Since these structures occurred in the retinular cells of mutant eyes, they were considered to be multivesicular bodies responsible for the reduction in rhabdom volume in the presence of light, as a type of adaptation to brightness. The reduction of pigment in the pigmentary and retinular cells and the morphological changes seen in the rhabdom of the ommatidia may originate visual deficiencies, which could explain the behavioral modifications reported for Apis mellifera queens with mutant eye color.Este estudo descreve as diferenças ultra-estruturais entre os olhos compostos de rainhas de abelhas de genótipo ch li/ch li e Ch/ch li. Foram registradas diferenças na organização do omatídeo e na pigmentação geral dos dois tipos de olhos. As abelhas heterozigotas apresentaram organização ultra-estrutural dos omatídeos praticamente normal. Contudo, foram observadas algumas alterações nos cones cristalinos, particularmente a presença de pequenos vacúolos nas células dos cones e perda de pigmento pelas células pigmentares primárias. Nas abelhas homozigotas foram encontrados vários omatídeos com cones cristalinos muito

  3. Chemical analysis of solid materials by a LIMS instrument designed for space research: 2D elemental imaging, sub-nm depth profiling and molecular surface analysis

    Science.gov (United States)

    Moreno-García, Pavel; Grimaudo, Valentine; Riedo, Andreas; Neuland, Maike B.; Tulej, Marek; Broekmann, Peter; Wurz, Peter

    2016-04-01

    Direct quantitative chemical analysis with high lateral and vertical resolution of solid materials is of prime importance for the development of a wide variety of research fields, including e.g., astrobiology, archeology, mineralogy, electronics, among many others. Nowadays, studies carried out by complementary state-of-the-art analytical techniques such as Auger Electron Spectroscopy (AES), X-ray Photoelectron Spectroscopy (XPS), Secondary Ion Mass Spectrometry (SIMS), Glow Discharge Time-of-Flight Mass Spectrometry (GD-TOF-MS) or Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) provide extensive insight into the chemical composition and allow for a deep understanding of processes that might have fashioned the outmost layers of an analyte due to its interaction with the surrounding environment. Nonetheless, these investigations typically employ equipment that is not suitable for implementation on spacecraft, where requirements concerning weight, size and power consumption are very strict. In recent years Laser Ablation/Ionization Mass Spectrometry (LIMS) has re-emerged as a powerful analytical technique suitable not only for laboratory but also for space applications.[1-3] Its improved performance and measurement capabilities result from the use of cutting edge ultra-short femtosecond laser sources, improved vacuum technology and fast electronics. Because of its ultimate compactness, simplicity and robustness it has already proven to be a very suitable analytical tool for elemental and isotope investigations in space research.[4] In this contribution we demonstrate extended capabilities of our LMS instrument by means of three case studies: i) 2D chemical imaging performed on an Allende meteorite sample,[5] ii) depth profiling with unprecedented sub-nm vertical resolution on Cu electrodeposited interconnects[6,7] and iii) preliminary molecular desorption of polymers without assistance of matrix or functionalized substrates.[8] On the whole

  4. LIMS for Lasers 2015 for achieving long-term accuracy and precision of δ2H, δ17O, and δ18O of waters using laser absorption spectrometry

    Science.gov (United States)

    Coplen, Tyler B.; Wassenaar, Leonard I

    2015-01-01

    RationaleAlthough laser absorption spectrometry (LAS) instrumentation is easy to use, its incorporation into laboratory operations is not easy, owing to extensive offline manipulation of comma-separated-values files for outlier detection, between-sample memory correction, nonlinearity (δ-variation with water amount) correction, drift correction, normalization to VSMOW-SLAP scales, and difficulty in performing long-term QA/QC audits.MethodsA Microsoft Access relational-database application, LIMS (Laboratory Information Management System) for Lasers 2015, was developed. It automates LAS data corrections and manages clients, projects, samples, instrument-sample lists, and triple-isotope (δ17O, δ18O, and δ2H values) instrumental data for liquid-water samples. It enables users to (1) graphically evaluate sample injections for variable water yields and high isotope-delta variance; (2) correct for between-sample carryover, instrumental drift, and δ nonlinearity; and (3) normalize final results to VSMOW-SLAP scales.ResultsCost-free LIMS for Lasers 2015 enables users to obtain improved δ17O, δ18O, and δ2H values with liquid-water LAS instruments, even those with under-performing syringes. For example, LAS δ2HVSMOW measurements of USGS50 Lake Kyoga (Uganda) water using an under-performing syringe having ±10 % variation in water concentration gave +31.7 ± 1.6 ‰ (2-σ standard deviation), compared with the reference value of +32.8 ± 0.4 ‰, after correction for variation in δ value with water concentration, between-sample memory, and normalization to the VSMOW-SLAP scale.ConclusionsLIMS for Lasers 2015 enables users to create systematic, well-founded instrument templates, import δ2H, δ17O, and δ18O results, evaluate performance with automatic graphical plots, correct for δ nonlinearity due to variable water concentration, correct for between-sample memory, adjust for drift, perform VSMOW-SLAP normalization, and perform long-term QA/QC audits

  5. LIMS for Lasers 2015 for achieving long-term accuracy and precision of δ2H, δ17O, and δ18O of waters using laser absorption spectrometry

    Science.gov (United States)

    Coplen, Tyler B.; Wassenaar, Leonard I

    2015-01-01

    Although laser absorption spectrometry (LAS) instrumentation is easy to use, its incorporation into laboratory operations is not easy, owing to extensive offline manipulation of comma-separated-values files for outlier detection, between-sample memory correction, nonlinearity (δ-variation with water amount) correction, drift correction, normalization to VSMOW-SLAP scales, and difficulty in performing long-term QA/QC audits. METHODS: A Microsoft Access relational-database application, LIMS (Laboratory Information Management System) for Lasers 2015, was developed. It automates LAS data corrections and manages clients, projects, samples, instrument-sample lists, and triple-isotope (δ(17) O, δ(18) O, and δ(2) H values) instrumental data for liquid-water samples. It enables users to (1) graphically evaluate sample injections for variable water yields and high isotope-delta variance; (2) correct for between-sample carryover, instrumental drift, and δ nonlinearity; and (3) normalize final results to VSMOW-SLAP scales. RESULTS: Cost-free LIMS for Lasers 2015 enables users to obtain improved δ(17) O, δ(18) O, and δ(2) H values with liquid-water LAS instruments, even those with under-performing syringes. For example, LAS δ(2) HVSMOW measurements of USGS50 Lake Kyoga (Uganda) water using an under-performing syringe having ±10 % variation in water concentration gave +31.7 ± 1.6 ‰ (2-σ standard deviation), compared with the reference value of +32.8 ± 0.4 ‰, after correction for variation in δ value with water concentration, between-sample memory, and normalization to the VSMOW-SLAP scale. CONCLUSIONS: LIMS for Lasers 2015 enables users to create systematic, well-founded instrument templates, import δ(2) H, δ(17) O, and δ(18) O results, evaluate performance with automatic graphical plots, correct for δ nonlinearity due to variable water concentration, correct for between-sample memory, adjust for drift, perform VSMOW-SLAP normalization, and

  6. Comparación del "Tabog" (Swinglea glutinosa Merr con el limón "rugoso" (Citrus jambhiri Swingle y la mandarina "Cleopatra" (C. reticulata Swingle como porta-injerto para la lima acida ''Tahiti" (C. aurantifolia Swingle

    Directory of Open Access Journals (Sweden)

    Restrepo C. Orlando

    1987-09-01

    Full Text Available At the C.N.I.-I.C.A. Palmira, fruit trees program the influence of "Tabog" Swinglea glutinosa was evaluated as rootstock for acid lime "Tahiti" Citrus aurantifolia, taking into account the following aspects: development and productivility of the trees quality and growth of fruits, demands of nutriments, and resistance or susceptibility to pest and disease; comparing its behavior with the commercial rootstocks rough, lemon C. jambhiri and mandarina, "Cleopatra" C. reticulata.En el C.N.l. Palmira del Instituto Colombiano Agropecuario (lCA, se evaluó la influencia del "Tabog" Swinglea glutinosa como porta-injerto para la lima "Tahiti " Citrus aurantifolia, teniendo en cuenta los siguientes aspectos: desarrollo y productividad del árbol, calidad y desarrollo de los frutos, exigencias de nutrimentos, resistencia o susceptibilidad a plagas y enfermedades; comparando su comportamiento con los porta-injertos comerciales limón "Rugoso" C. jambhiri y la mandarina "Cleopatra " C. reticulata.

  7. Problemas y métodos para la enseñanza del inglés como segunda lengua a los hablantes del mek-a-tel-yu en la provincia de Limón

    OpenAIRE

    Wright M, Fernando

    2006-01-01

    En este estudio presentaré un esbozo descriptivo del inglés criollo conocido como mek-a-tél-yu (M) que se habla en la provincia de Limón. Mencionaré algunos de los obstáculos que el hablante de esta lengua encuentra en el aprendizaje del inglés normativo que se enseña a nivel de secundaria en Costa Rica y sugeriré métodos y técnicas que el educador podría implementar para mejorar la enseñanza del inglés. 

  8. Assessing water salinity along River Limón and Caño San Miguel irrigation paleochannel (Maracaibo, Venezuela) as affected by the balance of soluble salts in alluvium soils

    Science.gov (United States)

    Rodriguez, Raquel; Moreno, Juan; Hermosilla, Daphne; Gascó, Antonio

    2013-04-01

    The saline degradation of soils that are irrigated with brackish water is worrisome increasing worldwide, and it may further affect the salinity of fresh water in those streams flowing across. The problem that is caused by an increasing concentration of salts that are more soluble than gypsum depends on the quality of irrigation water, climatic aridity, and drainage limitations. All these conditions meet in the alluvium soils of River Limón basin that are crossed by Caño San Miguel irrigation paleochannel. River Limón's regulation by closing Manuelote and Tulé artificial reservoirs has diminished the input of water and sediments from flooding events, which exerted dilutive effects in the past. In addition, the balance of soluble salts in these soils has also registered further net accumulation during those extremely dry years happened before 2006, because the great dilution contribution of ombrogenic dammed water coming from rain has not been enough to compensate salts concentration generated by water evapotranspiration in those irrigated soils of the middle basin, particularly in the absence of superficial runoff and deep drainage. Considering those semi-arid climate conditions prevailing in the area (annual precipitation = 710 mm; potential evapotransporation = 2361 mm), it resulted that water analyses in River Limón showed a ten-fold increased maximum annual salinity concentration (March) along the stream; that is, an electric conductivity (Ce) of 0.37 dS•m-1 (at 25 °C) at Puente Carrasquero pumping station, where water for crop irrigation is subtracted, turns to 34.60 dS•m-1 (at 25 °C) at its base level in Puerto Mara, where it discharges to Lake Maracaibo. In addition, the quality of irrigation water from Caño San Miguel, which aggregates to those coming from River Limón at the pumping station located in Carrasquero just before running through the alluvium of this water stream, resulted pretty irregular. In short, it spanned form C1 to C4 soil

  9. Impacto económico de la Ley Federal de Sanidad Vegetal en el mercado mexicano de limón persa Economic impact of the Federal Plant Health Law on the mexican market for persian lemon

    Directory of Open Access Journals (Sweden)

    Marlene Herbert Ruíz

    Full Text Available La globalización comercial exige que los mercados mundiales de productos agroalimentarios, realicen prácticas de inocuidad en sus productos libres de contaminantes químicos, biológicos y físicos. En este contexto, México modificó el 26 de julio de 2007 la Ley Federal de Sanidad Vegetal, la cual ahora tiene como objetivo la aplicación, verificación y certificación de los sistemas de reducción de riesgos de contaminación en la producción y empaque de vegetales. El objetivo de este trabajo fue analizar el impacto económico, que tiene la aplicación inmediata de esta ley en la oferta de limón persa mexicano y en consecuencia, el ingreso de los productores de este cítrico en 2007. Para ello se realizó un estudio de campo (encuestas en el municipio de Martínez de la Torre, Veracruz; principal zona productora de limón persa a nivel nacional. Además, se construyó un modelo probit que estableció los principales factores determinantes en la adopción de buenas prácticas agrícolas; siendo el rendimiento que presentó mayor efecto marginal (2.08%. Se planteó un escenario donde ante un incremento del rendimiento promedio 10% (ceteris paribus, se obtuvo un cambio de 342 personas en la población dispuestas a adoptar las buenas prácticas agrícolas; y en consecuencia, la oferta de limón persa se incrementaría 3.98%. Lo anterior manifiesta que si se acompaña la producción de limón persa con políticas que eleven el rendimiento, las buenas prácticas agrícolas aumentarán y por ende, la exportación de dicho producto.Commercial globalization demands that the world markets of food and agriculture products carry out innocuity practices in their products free of chemical, biological and physical pollutants. In this context, on July 26th., 2007, Mexico modified its Federal Plant Health Law, which now aims the aplication, verification and certification of the pollution risk reduction systems in the production and packaging of

  10. FY1995 optoelectronic devices and circuits for terabit class network; 1995 nendo terabit kyu network yo hikari denshi device kairo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The necessary technology realizing Terabit class optical network is the signal multiplexing both in temporal and spectral domains. Controllability of ultrafast optoelectronic systems is therefore the priority issue. Specifically we chose semiconductor lasers as the key devices. The methodology for performance improvement and the creation of novel functionality are investigated. Firstly compression of semiconductor laser pulse reached the world record of 65 femto second. Secondly a proposal was made to control mode locked semiconductor lasers via subharmonic synchronization and a substantial phase noise reduction was demonstrated. Thirdly a new technology was developed to realize broadband anti-reflection coating on semiconductor laser amplifier facet, bringing about significant performance improvement. To compensate the dispersion induced signal distortion a broadband semiconductor laser amplifier four wave mixing was analyzed and also demonstrated experimentally. (NEDO)

  11. Aplicación de ácido giberélico en precosecha y cera en poscosecha a frutos de limón mexicano Preharvest application of gibberellic acid and wax at postharvest in fruit of mexican lime

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    Rosario Álvarez-Armenta

    Full Text Available Este trabajo se efectuó en una huerta comercial ubicada en Tecomán, Colima, México durante 2006, para determinar el efecto precosecha de la aplicación de ácido giberélico (AG3 en frutos de limón mexicano. Se realizaron 3, 2, 1 y 0 aplicaciones de 10 mg L-1 de ácido giberélico a frutos en desarrollo a los 64, 78 y 92 días después de la antesis. Además, un día después de la cosecha, se aplicó una cubierta con cera de carnauba a la mitad de los frutos de cada tratamiento. El efecto del regulador de crecimiento y de la cera de carnauba en los frutos de limón se evaluó al final de un período de almacenamiento en refrigeración a 9 °C, durante 35 días y de la exposición durante siete días a 20 ºC, para simular condiciones de comercialización. Los parámetros considerados para determinar la acción de los reguladores de crecimiento exógenos fueron: índice de color; sólidos solubles totales (ºBx, acidez titulable, ácido ascórbico y pérdida de peso. Los resultados obtenidos revelaron que el tratamiento de tres aplicaciones de 10 mg L-1 de AG3 + cera, mantuvo las características de calidad de los frutos de limón al final de ambos períodos de almacenamiento. Así, la combinación del regulador y la cera retrasaron la senescencia, principalmente durante los primeros 35 días de almacenamiento bajo condiciones de refrigeración. Después de ese período, en todos los tratamientos se observó una reducción en el contenido de vitamina C y acentuados cambios en la coloración del fruto.This work was conducted during 2006 in a commercial orchard located in Tecomán, Colima, Mexico, in order to determine the effect of preharvest application of gibberellic acid (GA3 to mexican lime fruits. Sprays with 10 mg L-1 gibberellic acid, were made 3, 2, 1 and 0 times to developing fruits at 64, 78 and 92 days after anthesis. In addition, a day after harvest, a carnauba wax cover was applied to half of the fruits from each treatment. The

  12. Calagem e adubação fosfatada favorecem o crescimento do capim-limão, Cymbopogon citratus (DC Stapf Liming and phosphate fertilization promote lemongrass, Cymbopogon citratus (DC Stapf., growth

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    C.V.T. Amarante

    2012-01-01

    Full Text Available O capim-limão, Cymbopogon citratus (DC Stapf., é uma planta utilizada para fins medicinais e aromáticos, porém pouco se conhece sobre as exigências nutricionais. O objetivo do presente trabalho foi avaliar os efeitos da elevação do pH do solo e da adubação com fósforo (P na disponibilidade de nutrientes e no crescimento inicial do capim-limão. O experimento foi conduzido em Lages, SC, de abril a dezembro de 2008, em casa de vegetação. Foi utilizado um esquema fatorial, incluindo quatro níveis de pH (4,1; 5,5; 6,0 e 6,5 e três doses de P (0, 50 e 100 mg kg-1 de solo, com quatro repetições (correspondendo a um vaso com duas plantas. A correção do pH aumentou o Ca e Mg e diminuiu o Al, Mn, Fe, Cu e Zn no solo. A aplicação de 100 mg kg-1 de P elevou em mais de 250% a concentração deste nutriente no solo, independente do pH. A aplicação de P e, principalmente, a elevação do pH do solo incrementaram todos os atributos avaliados nas plantas de capim limão (massa seca da parte aérea e do sistema radicular, número de folhas e altura de plantas, sendo que a interação entre pH e dose de P somente foi significativa para o número de folhas. O maior crescimento das plantas ocorreu no tratamento com pH 6,5, associado à adição de 100 mg kg-1 de P.Lemongrass, Cymbopogon citratus (DC Stapf., is a plant with medicinal and aromatic uses. However, little is known about its nutrition requirements. This work was carried out to investigate the effects of pH and phosphorus levels on nutrients availability and the initial growth of lemongrass. The experiment was carried out in Lages (SC, Brazil from April tol December 2008, in greenhouse conditions. The experiment followed a factorial design, with four levels of pH (4.1, 5.5, 6.0, and 6.5, three doses of phosphorus (0, 50, and 100 mg kg-1 of soil, and four replicates (each replicate corresponding to a pot with two plants. The correction of pH increased Ca and Mg, and reduced Al, Mn, Fe

  13. Características físico-químicas y determinación de plaguicidas en el agua de la laguna de Gandoca, Limón, Costa Rica

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    Marta Coll

    2004-12-01

    Full Text Available Parámetros físico-químicos fueron determinados en tres puntos de muestreo y a dos profundidades entre noviembre 1999 y abril 2000, en la laguna de Gandoca, Refugio Nacional de Vida Silvestre Gandoca-Manzanillo, Limón, Costa Rica. Se determinó la temperatura, turbidez, salinidad, nutrimentos y clorofilas en la laguna. Se detectó una haloclina presente a 3 m de profundidad y se describió una circulación de aguas con fuerte estratificación por haloclina pero con una interfase entre la laguna y el mar de aguas parcialmente mezcladas. No se detectaron procesos de eutrofización. La fluctuación observada en los nutrimentos y clorofilas correspondería a procesos de circulación normales y complejos en la laguna, claramente influenciada por las aguas continentales. Además, durante febrero 2000, se realizó un estudio preliminar para la detección de sustancias organocloradas y organofosforadas en los puntos de muestreo. El análisis de residuos realizado mediante extracción líquido-líquido no detectó la presencia en las aguas de la laguna de ninguna de las veinte sustancias consideradas. La no detección de éstas podría indicar que no están llegando a la laguna a través del agua de escorrentía, o bien que una vez presentes en la laguna han sido lavadas por las fuertes lluvias durante el muestreoNutrients and chlorophylls concentrations, as well as salinity, temperature and Secchi disk depth were determined from November 1999 to April 2000, at three stations and two depths, at Gandoca lagoon, Gandoca-Manzanillo National Wildlife Refuge, Limón, Costa Rica. Salinity profiles indicated that the lagoon was a salt wedge estuary with a partially mixed region near the mouth. No processes of eutrophication were found. The distribution and abundance of nutrients and chlorophylls showed a slight influence of continental water and water circulation patterns in the lagoon. A preliminary study was done in order to analyze the presence of 20

  14. Allelopathic effect of lemongrass and sabugueiro on Cuban jute germination/ Efeito alelopático de capim limão e sabugueiro sobre a germinação de guanxuma

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    Andréa Maria Teixeira Fortes

    2007-08-01

    Full Text Available The invasive species Cuban jute (Sida rhombifolia L. is common in no-till areas. It is an important competitor of crops because of a quite effective root system. For this reason, Cuban jute control commonly is made on large-scale applications of chemicals. This study seeks to make a contribution to the control of Cuban jute via allelopathy using hot aqueous extracts of the medicinal species lemongrass (Cymbopogon citratus Stapf. and sabugueiro (Sambucus australis Cham & Schltdl.. The experiment was carried out in two complementary steps: first we attempted to overcome dormancy of the invasive species, and then we investigated the effect of the medicinal species lemongrass and sabugueiro on Cuban jute germination. The seeds were placed in Petri dishes previously autoclaved for 20 minutes at 121°C and then taken to a germination chamber with a photoperiod of 12 hours at 25°C. A potential allelopathic, inhibitory effect was identified on Cuban jute seed germination when the weed was exposed to the hot aqueous extract of both medicinal species.A espécie invasora guanxuma (Sida rhombifolia L. é comum em áreas de plantio direto e destaca-se na competição com a cultura agrícola por apresentar um sistema radicular bastante eficiente; para tanto, seu controle depende comumente de aplicações químicas em larga escala. O presente estudo teve como objetivo avaliar o efeito alelopático do extrato aquoso quente das espécies medicinais capim limão (Cymbopogon citratus Stapf. e sabugueiro (Sambucus australis Cham & Schltdl. sobre a germinação da espécie invasora guanxuma. O experimento foi realizado em duas etapas complementares: primeiramente visou-se à quebra de dormência da espécie invasora e depois, verificou-se o efeito de capim limão e sabugueiro sobre a germinação de guanxuma. O acondicionamento das sementes foi realizado em placas de Petri previamente autoclavadas por 20 minutos a 121°C, dispostas em Câmara de Germinação com

  15. Human T-Cell Leukemia Virus Type I-Mediated Repression of PDZ-LIM Domain-Containing Protein 2 Involves DNA Methylation But Independent of the Viral Oncoprotein Tax

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    Pengrong Yan

    2009-10-01

    Full Text Available Human T-cell leukemia virus type I (HTLV-I is the etiological agent of adult T-cell leukemia (ATL. Our recent studies have shown that one important mechanism of HTLV-I-Mediated tumorigenesis is through PDZ-LIM domain-containing protein 2 (PDLIM2 repression, although the involved mechanism remains unknown. Here, we further report that HTLV-I-Mediated PDLIM2 repression was a pathophysiological event and the PDLIM2 repression involved DNA methylation. Whereas DNA methyltransferases 1 and 3b but not 3a were upregulated in HTLV-I-transformed T cells, the hypomethylating agent 5-aza-2′-deoxycytidine (5-aza-dC restored PDLIM2 expression and induced death of these malignant cells. Notably, the PDLIM2 repression was independent of the viral regulatory protein Tax because neither short-term induction nor long-term stable expression of Tax could downregulate PDLIM2 expression. These studies provide important insights into PDLIM2 regulation, HTLV-I leukemogenicity, long latency, and cancer health disparities. Given the efficient antitumor activity with no obvious toxicity of 5-aza-dC, these studies also suggest potential therapeutic strategies for ATL.

  16. LIMS在实验室认证和规范化管理中的应用模型%The Application Model of LIMS in the Laboratory Qualification and Standardized Management

    Institute of Scientific and Technical Information of China (English)

    陈婷; 杨铸; 张改荣; 陈舜琮

    2009-01-01

    结合实验室认证和规范化管理的需求,对实验室信息管理系统LIMS(Laboratory Information Management System)以结构化分析方法进行系统建模,探索分析检测领域的一般应用模型.根据分析检测技术5T模型,抽象出LIMS的系统输入/输出数据实体.通过采取自上而下的设计思路对输入/输出数据流的加工进行逐层细化,形成LIMS各子系统的数据流图,最终在数据流图的基础上实现LIMS在实验室认证和规范化管理中的系统功能框架.

  17. LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori.

    Science.gov (United States)

    Kimoto, Mai; Tsubota, Takuya; Uchino, Keiro; Sezutsu, Hideki; Takiya, Shigeharu

    2015-01-01

    In the silkworm Bombyx mori, three fibroin genes, fibroin-heavy-chain (fibH), fibroin-light-chain (fibL) and fibrohexamerin (fhx), are coexpressed only in the posterior silk gland (PSG) cells, while the sericin genes encoding silk glue proteins are expressed in the middle silk gland (MSG) cells. Silk gland factor-2 (SGF-2) is a PSG-specific activator complex of fibH, composed of a LIM-homeodomain protein, Awh, and its cofactors, Ldb and Lcaf. We investigated whether SGF-2 can activate other fibroin genes using transgenic silkworms. The genes for Ldb and Lcaf were expressed ubiquitously in various tissues, while the gene for Awh was expressed strictly specific in PSG of the wild type silkworms. Misexpression of Awh in transgenic silkworms induced ectopic expression of fibL and fhx as well as fibH in MSG. Coincidently with the induction of fibL and fhx by Awh, binding of SGF-2 to the promoter of fibL and fhx was detected in vitro, and SGF-2 binds directly to the fhx core promoter. Ectopic expression of the fibroin genes was observed at high levels in the middle part of MSG. Moreover, fibL and fhx were induced in the anterior silk gland (ASG) of the transgenic silkworms, but fibH was not. These results indicate that Awh is a key activator of all three fibroin genes, and the activity is probably regulated in conjunction with additional factors.

  18. Sea-ice evaluation of NEMO-Nordic 1.0: a NEMO–LIM3.6-based ocean–sea-ice model setup for the North Sea and Baltic Sea

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    P. Pemberton

    2017-08-01

    Full Text Available The Baltic Sea is a seasonally ice-covered marginal sea in northern Europe with intense wintertime ship traffic and a sensitive ecosystem. Understanding and modeling the evolution of the sea-ice pack is important for climate effect studies and forecasting purposes. Here we present and evaluate the sea-ice component of a new NEMO–LIM3.6-based ocean–sea-ice setup for the North Sea and Baltic Sea region (NEMO-Nordic. The setup includes a new depth-based fast-ice parametrization for the Baltic Sea. The evaluation focuses on long-term statistics, from a 45-year long hindcast, although short-term daily performance is also briefly evaluated. We show that NEMO-Nordic is well suited for simulating the mean sea-ice extent, concentration, and thickness as compared to the best available observational data set. The variability of the annual maximum Baltic Sea ice extent is well in line with the observations, but the 1961–2006 trend is underestimated. Capturing the correct ice thickness distribution is more challenging. Based on the simulated ice thickness distribution we estimate the undeformed and deformed ice thickness and concentration in the Baltic Sea, which compares reasonably well with observations.

  19. Sea-ice evaluation of NEMO-Nordic 1.0: a NEMO-LIM3.6-based ocean-sea-ice model setup for the North Sea and Baltic Sea

    Science.gov (United States)

    Pemberton, Per; Löptien, Ulrike; Hordoir, Robinson; Höglund, Anders; Schimanke, Semjon; Axell, Lars; Haapala, Jari

    2017-08-01

    The Baltic Sea is a seasonally ice-covered marginal sea in northern Europe with intense wintertime ship traffic and a sensitive ecosystem. Understanding and modeling the evolution of the sea-ice pack is important for climate effect studies and forecasting purposes. Here we present and evaluate the sea-ice component of a new NEMO-LIM3.6-based ocean-sea-ice setup for the North Sea and Baltic Sea region (NEMO-Nordic). The setup includes a new depth-based fast-ice parametrization for the Baltic Sea. The evaluation focuses on long-term statistics, from a 45-year long hindcast, although short-term daily performance is also briefly evaluated. We show that NEMO-Nordic is well suited for simulating the mean sea-ice extent, concentration, and thickness as compared to the best available observational data set. The variability of the annual maximum Baltic Sea ice extent is well in line with the observations, but the 1961-2006 trend is underestimated. Capturing the correct ice thickness distribution is more challenging. Based on the simulated ice thickness distribution we estimate the undeformed and deformed ice thickness and concentration in the Baltic Sea, which compares reasonably well with observations.

  20. Tratamento farmacológico do transtorno de personalidade limítrofe: revisão crítica da literatura e desenvolvimento de algoritmos Tratamiento farmacológico del trastorno de personalidad limítrofe: revisión crítica de la literatura y desarrollo de algoritmos Pharmacological treatment of borderline personality disorder: critical review of the literature and development of algorithms

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    André F. Carvalho

    2004-08-01

    Full Text Available INTRODUÇÃO: O transtorno de personalidade limítrofe (TPL é uma síndrome psiquiátrica que causa significativa morbidade e mortalidade para pacientes internados e ambulatoriais tratados por profissionais de saúde mental. Dada a sua alta prevalência na população de pacientes psiquiátricos, esforços são necessários para tratar esse grupo apropriadamente. OBJETIVO: O objetivo do presente artigo é revisar evidências atuais acerca da efetividade das diversas abordagens farmacológicas empregadas como parte do plano de tratamento produzido para indivíduos que enfrentam essa doença. MÉTODOS: Para esse propósito, os autores revisaram o banco de dados MEDLINE em busca de ensaios clínicos publicados entre 1986 e 2003. Borderline personality disorder e clinical trials foram usados com descritores. RESULTADOS: Numerosos trabalhos foram encontrados, mas poucos estudos controlados estavam disponíveis. Notável é o fato de que virtualmente todas as classes de psicofármacos foram testadas nesse grupo de pacientes, comumente com desfechos modestos ou variáveis. Devido a limitações metodológicas na vasta maioria dos ensaios clínicos envolvendo drogas específicas nesse grupo de pacientes, foi difícil estimar o benefício real dos vários agentes testados. A evidência mais forte existe para os inibidores seletivos de serotonina, antipsicóticos e divalproato. DISCUSSÃO: Claramente, mais ensaios clínicos bem delineados são necessários, mas os dados revisados sugerem que psicofármacos são efetivos para tratar sintomas-alvo nesses pacientes. Conclui-se que psicotrópicos são adjuntos úteis às abordagens psicoterapêuticas usualmente empregadas para tratar pacientes limítrofes.INTRODUCCÍON: El trastorno de personalidad limítrofe (TPL es un síndrome psiquiátrico que causa significativa morbididad y mortalidad a pacientes internados y ambulatoriales tratados por profesionales de salud mental. Vista su alta prevalencia en la

  1. Redução de esmalte interproximal como alternativa no tratamento ortodôntico de casos limítrofes Interproximal enamel stripping as orthodontic treatment alternative in borderline cases

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    Alberto Rossi Júnior

    2009-04-01

    Full Text Available INTRODUÇÃO: o plano de tratamento ortodôntico deve considerar fatores como apinhamento dentário, protrusão dentoalveolar, discrepâncias maxilares e necessidade de alteração do perfil facial. Também se deve ter em mente as características de normalidade conforme a raça do paciente. Assim, buscando uma abordagem individual do paciente, em casos limítrofes, a terapia ortodôntica pode ser realizada com ou sem extrações. OBJETIVO: realizar uma revisão da literatura sobre a terapia de redução de esmalte interproximal como uma alternativa de tratamento ortodôntico sem extrações para pacientes considerados limítrofes e exemplificar a técnica a partir do relato de um caso clínico. MÉTODOS: para a execução da redução, usou-se brocas Carbide em alta rotação para a remoção do esmalte, discos Sof-Lex para o acabamento e polimento das superfícies desgastadas e aplicação tópica de fluoretos, visando aumentar a proteção do esmalte. CONCLUSÃO: concluiu-se que a técnica é indicada na resolução de discrepâncias no comprimento do arco dentário de até 8,5mm, a fim de evitar as extrações dentárias em pacientes com bom perfil facial. Preferencialmente, os dentes submetidos à técnica devem ser largos, com forma triangular e paredes de esmalte espessas.INTRODUCTION: The orthodontic treatment plan must consider factors like dental crowding, dental alveolar protrusion, maxillary discrepancies and the facial profile change necessity. It must be considered that the normal characteristics changes in conformity with the ethnic origin of the patient. Then, searching for the individualization of treatment for each patient, in borderlines cases the orthodontic therapy can be done with or without extractions. AIM: To review the literature about the interproximal enamel reduction therapy as an orthodontic treatment alternative to extractions in this borderline patients and to exemplify the technic with a case report. METHODS: To

  2. Hasan İlmi of the Crete and His Divanche Titled Me'alimü'l-garam / Giritli Hasan İlmî ve Me'âlimü'l-garâm Adlı Divançesi

    Directory of Open Access Journals (Sweden)

    İsmail AVCI

    2014-03-01

    Full Text Available Crete Island is one of the most significant place by virtue of Turkish cultural history and often remembered by the authors and poets who lived in this island during the Ottoman period. The subject of this paper Hasan İlmi is amongst such authors commemorated as of the Crete. Hasan İlmi of the Crete worked as a government officer in various places in the Ottoman Empire and wrote several works of which only three works are available today. The first of these works is an unpublished Ottoman dictionary titled Osmanlı Dili. The second work of the author is titled Yemen Salnamesi with co-writer Hüseyin Hüsnü written during the period when the author served in Yemen. The last work of Hasan İlmi is a divanche (collection of his poems titled Me'alimü'l-garam. This work is composed of 2 praises dedicated to the Prophet Muhammad, 24 odes, 1 poem, and 3 ballads. In this paper, information about Hasan İlmi of the Crete and works will be provided and then his work Me'alimü'l-garam will be presented in more detail. Girit Adası, Osmanlı Dönemi'nde yetiştirdiği yazar ve şairlerle adından çokça söz edilen, Türk kültür tarihi bakımından dikkate değer yerlerden biridir. Bu yazıya konu olan Hasan İlmî de "Giridî" olarak anılan müelliflerdendir. Hayatını imparatorluğun farklı yerlerinde çeşitli devlet görevlerinde bulunarak geçiren Giritli Hasan İlmî'nin elde üç eseri bulunmaktadır. Bunlardan ilki Osmanlı Dili adlı basılamamış bir sözlük çalışmasıdır. İkinci eser müellifin Yemen'de bulunduğu dönemde Hüseyin Hüsnü ile birlikte hazırladığı Yemen Salnâmesi'dir. Hasan İlmî'nin üçüncü eseri ise şiirlerini bir araya getirdiği Me'âlimü'l-garâm adlı divançesidir. Eserde 2 naat, 24 gazel, 1 manzume ve 3 şarkı bulunmaktadır. Bu yazıda önce Giritli Hasan İlmî ve eserleri hakkında bilgi verilecek, ardından Me'âlimü'l-garâm üzerinde durulacak ve eserin metni verilecektir.

  3. Obtenção de híbridos somáticos de limão 'Cravo' e tangerina 'Cleópatra' Somatic hybridization between 'Rangpur' lime and 'Cleópatra' mandarin

    Directory of Open Access Journals (Sweden)

    Rodrigo Rocha Latado

    2002-12-01

    Full Text Available Este trabalho teve como objetivo a obtenção de híbridos somáticos entre o limão 'Cravo' (Citrus limonia Osbeck e a tangerina 'Cleópatra' (Citrus reshni Hort., para serem usados como porta-enxertos de citros. O limão 'Cravo' é atualmente o principal porta-enxerto comercial utilizado no Brasil, em virtude de suas boas qualidades agronômicas. Entretanto, é suscetível ao "declínio" dos citros, doença responsável pela eliminação anual de milhões de plantas cítricas no Brasil. A tangerina 'Cleópatra' é uma espécie bastante utilizada como porta-enxerto em outros países e tem sido descrita na literatura como tolerante ao "declínio". Protoplastos de suspensões celulares embriogênicas e protoplastos derivados de tecidos foliares foram utilizados para fusão com solução de PEG (50% e posterior cultivo em agarose. A porcentagem de obtenção de células híbridas interespecíficas logo após a fusão, variou entre 5,1% e 6,8%. Foram obtidas mais de 500 plantas a partir de produtos de fusão cultivados em gotas de agarose. No total de 180 plantas avaliadas, 11 híbridos somáticos foram discriminados e confirmados, utilizando-se marcadores moleculares RAPD e isoenzimáticos (sistemas PO, IDH e PGI. Seis plantas foram aclimatizadas, plantadas no solo e estão sendo multiplicadas por estaquia para avaliação futura como porta-enxertos de citros.The aim of this work was to obtain somatic hybrids between 'Rangpur' lime (Citrus limonia Osbeck and 'Cleópatra' mandarin (Citrus reshni Hort., to be used as a citrus rootstock. 'Rangpur' lime is the most important Brazilian citrus rootstock due to its higher horticultural performance. Nevertheless, this species is susceptible to blight disease, which is responsible for the death of millions of productive trees per year in Brazil. 'Cleópatra' mandarin has become an increasingly important rootstock in other countries and has been reported as being tolerant to citrus blight disease. Leaf

  4. “郁结”下的“求索”--叶维廉“文化模子”理论%The Search under the Depression:Cultural Mould Theory of Yip Wai-lim

    Institute of Scientific and Technical Information of China (English)

    陆志胜

    2016-01-01

    针对中国在现代性进程中所面临的文化主体性丧失的危机,叶维廉先生提出的”文化模子“理论具有启发性的意义:在理论层面上否决和消解了跨文化交流中的文化中心主义,通过多元文化的平等对话,来促进彼此间的”互照互识“;揭示了现代性进程下,西方工业文化对非西方国家民族意识弱化和文化独特性破坏的危害以及其中潜藏的某种权力关系,在方法论的层面,为我们反思中国的现代性进程和重塑当代中国的文化主体性提供了指导。%In face of the crisis of loss of cultural subjectivity during the process of modernity,Yip Wai—lim put forward Cultural Mould Theory,which has two great meanings:First,the meaning of Cross-cultural communication,which will conducive to realize Inter—cogmition of multi—culture;The second,under the wave of Globalization,the impact of western cultural hegemony has weaken the national conscious-ness,which has damaged the cultural diversity. Cultural Mould Theory has responsed to the cultural appeals,which contains the resistance of other nations to the impact of western culture,to establish and maintain its own cultural subjectivity,avoiding being the Others who were ruled and assimilated.

  5. Estudio Preliminar de las Propiedades de la Semilla de Limón Mexicano (Citrus aurantifolia swingle para su Posible Aprovechamiento Preliminary Study of the Properties of Mexican Lime Seed (Citrus aurantifolia swingle for its Possible Uses

    Directory of Open Access Journals (Sweden)

    Enrique Arriola-Guevara

    2006-01-01

    Full Text Available Se obtuvieron propiedades clave de la semilla de limón mexicano (Citrus aurantifolia swingle para su posible aprovechamiento en la industria de alimentos. Se consideraron propiedades físicas, composición, toxicidad en roedores (prueba de dosis letal media y toxicidad crónica y actividad germicida (prueba de halos de inhibición y efectividad germicida. La semilla contiene 21% de proteínas, 39% de aceite vegetal y 29% de fibra, especialmente lignina en cantidad considerable, lo que hace viable su uso como materia prima para nutracéuticos o alimentos funcionales. La toxicidad de la semilla resultó nula por lo que es apta para el consumo humano. Se encontró cierta efectividad germicida para Candida albicans 85.77% (población Inicial = 0.304*10(8 UFC/ml y para Escherichia coli 91.447% (población Inicial = 190*10(8 UFC/ml, por lo que puede ser usada como ingrediente en germicidas.Key properties of seed of Mexican lime (Citrus aurantifolia swingle were studied for potential uses by the food industry. Characteristics considered included physical properties, composition, toxicity to rodents (average lethal dose and chronic toxicity, as well as germicidal activity (inhibition rings and germicidal effectiveness. The seed contained 21% protein, 39% vegetable oil and 29% fiber, particularly lignin. The seed could potentially be used as a basic component for nutritional supplements or functional food products. The toxicity was nil, and thus seed derivatives were safe for human consumption. Some germicidal effectiveness was found against Candida albicans, 85.77% (starting population = 0.304*10(8 UFC/ml, and against Escherichia coli, 91.447% (starting population = 190*10(8 UFC/ml, suggesting seed extracts could be used as an ingredient in germicidal products.

  6. Expression of Muscle LIM Protein Gene from Monochamus alternatus in Response to 11 Kinds of Pesticides%11种杀虫剂对松墨天牛肌肉LIM蛋白基因表达的影响

    Institute of Scientific and Technical Information of China (English)

    罗淋淋; 林同

    2014-01-01

    松墨天牛(Monochamusalternatus Hope)是松树的重要蛀干害虫,也是松材线虫(Bursaphelenchusxylophilus)的主要传播媒介。试验从松墨天牛文库中分离得到肌肉LIM蛋白基因的部分cDNA序列,长度为871 bp,命名为MaLIM(GenBank:KJ872589);与赤拟谷盗(Tribolium castaneum)和家蚕(Bombyxmori)氨基酸相似性分别为77%和75%。用RT-qPCR测定了11种杀虫剂对MaLIM相对表达量的影响,结果表明,杀虫剂处理后MaLIM基因的表达量均有所下降,仅为对照的0.03~0.53。%Monochamus alternatus H ope is one of the important pests of pine stemborers,and also is vector of pine wood nematode (Bursaphelenchusxylophilus).Inthisstudy,apartialcDNA sequenceofmuscleLIMproteingene with870bpinlengthwasisolatedfromM.alternatuslibrary and named MaLIM(G enB ank:K J872589) thathas 77% and 75% amino acid similarity with the muscle L IMprotein of Triboliumcastaneumand Bombyx mori respectively. T he relative expression of MaLIMin response to 11 kinds of insecticides was determined by R T-qPC R ,the results showed thatthe MaLIMwas down regulated and the expression levelwas changed from0.03 times to 0.53 times of the control.

  7. Caracterización de la estructura de peces ornamentales durante dos períodos hidrológicos en el caño agua limón, departamento de Arauca, Colombia

    Directory of Open Access Journals (Sweden)

    Ruiz Moreno Andrés Ricardo

    2006-06-01

    Full Text Available Se realizó un inventarió de los peces ornamentales en el caño Agua Limón, en el municipio de Arauca, Arauca (Colombia, en los períodos comprendidos de febrero a mayo de 2005 y de septiembre a agosto de 2005. Como estrategia metodológica para el estudio se realizó un esfuerzo de captura de dos horas por jornada empleando como artes de pesca el trasmallo de fibra de nylon y el aro. Se registraron 4.753 individuos que
    corresponden a 74 especies pertenecientes a 21 familias donde las especies más abundantes fueron Ctenobrycon spilurus (22%, Astyanax sp. (18% y Gephyrocharax sp. (10%. Los órdenes con mayor representación específica fueron Characiformes y Siluruformes (reuniendo el 71% de las capturas. Las familias con mayor riqueza de especies fueron Characidae (24 spp. y Loricariidae (12 spp. seguidas por las familias Cichlidae (6 spp., Apteronotidae y Pimelodidae con cuatro especies, Auchenipteridae y Callichthydae con tres especies cada una. La mayor riqueza de especies se encontró en los meses de septiembre, agosto y marzo, se sugiere que estos resultados están relacionados con las variaciones del régimen hidrológico de esta región dando como consecuencia cambios de distribución en los cuerpos de agua que frecuentan las especies. El mes de mayo tiene la menor diversidad y al
    mayor abundancia de especies.

  8. Preferencias en la anidación de tortugas carey (Eretmochelys imbricata y baulas (Dermochelys coriacea en el Refugio Nacional de Vida Silvestre Gandoca Manzanillo, Limón, Costa Rica

    Directory of Open Access Journals (Sweden)

    Lilliana Piedra-Castro

    2015-09-01

    Full Text Available El Caribe costarricense es importante como sitio de alimentación, copulación, anidación y desove de las tortugas marinas, por lo que el presente trabajo pretendió actualizar el patrón de anidación en las tortugas marinas Dermochelys coriacea y Eretmochelys imbricata en el sector Gandoca, del Refugio Nacional de Vida Silvestre Gandoca Manzanillo, Limón, Costa Rica, para proponer medidas de manejo. Se realizaron recorridos nocturnos entre las 8:00 p. m. y 4:00 a. m., la playa se dividió en cinco sectores, en estos se realizaron observaciones directas y de rastros, de las tortugas que elaboraron el nido, entre febrero y agosto del 2011 y 2012. Se registraron tres especies de tortugas marinas, Dermochelys coriacea, Eretmochelys imbricata y Chelonia mydas. La anidación de tortugas baula tuvo su punto máximo entre marzo y julio; por su parte, las carey, entre mayo y junio (2011, y junio hasta agosto en el 2012. Se observó preferencia en la posición de la playa utilizada para construir sus nidos; la baula utiliza principalmente la parte media, mientras que la carey utilizó mayormente la parte baja para anidar (t = 17.2525. Se observó que la baula utilizó frecuentemente el sector C; sin embargo, no se encontraron diferencias en el uso de los sectores. Se concluye que ambas especies tienen preferencias en la selección de la zona de playa en que anidan; la carey en la parte baja cerca de la línea de costa y la baula en la parte media. Además, las tortugas baula utilizan indistintamente los diferentes sectores de la playa estudiada.

  9. Avaliação genética de seleções e híbridos de limões cravo, volkameriano e rugoso como porta-enxertos para laranjeiras Valência na presença da morte súbita dos citros Genetic evaluation of selections and hybrids of rangpur lime, volkamer and rough lemons rootstocks for Valência orange trees in the presence of the citrus sudden death

    Directory of Open Access Journals (Sweden)

    Jorgino Pompeu Junior

    2013-03-01

    Full Text Available Este estudo teve como objetivo realizar a avaliação genética da produção de frutos, eficiência produtiva e altura de laranjeiras Valência (Citrus sinensis enxertadas em seleções e híbridos dos limões Cravo (C. limonia, Volkameriano (C. volkameriana e Rugoso (C. jambhiri, em área endêmica para morte súbita dos citros (MSC. Foram avaliados 36 genótipos desses porta-enxertos, representados por cinco plantas cada, avaliados em cinco safras, do terceiro ao sétimo ano após o plantio. Sete dos genótipos avaliados apresentaram plantas com sintomas de MSC até o sétimo ano: Rangpur Otaheite orange 12901 (859, Rangpur Red Lime D.33.30 (866, Limão-Cravo EEL (871, Rangpur Borneo red (874, Citrus kokhai (1649, Limão-Rugoso 58329 (1655 e Limão- Cravo x Swingle B (1695. Para os genótipos que não manifestaram sintomas da doença, foram estimados parâmetros genéticos e fenotípicos e realizada a predição de valores genéticos dos indivíduos, visando à seleção e ao melhoramento genético para as características citadas, empregando-se o método REML/BLUP (máxima verossimilhança restrita/melhor predição linear não viciada. A análise de produção de frutos de cinco safras mostrou acurácia seletiva de 84,59%, tornando-se desnecessária a avaliação de maior número de safras. A seleção dos sete melhores genótipos proporcionou ganhos genéticos de 11,5% na produção de frutos, enquanto a do melhor genótipo conferiu ganho genético de 16,3%. As maiores médias genéticas preditas (>70,0 kg.pl-1 para produção de frutos foram obtidas pelos genótipos Limão-Cravo- Ipanema (1522, Santa- Bárbara-Red- Lime (884, Limão- Cravo- Limeira (863, Limão- Cravo- Taquaritinga (869, Limão- Rugoso- do -Cabo (1643, Rangpur- Rose Lime (868 e Limão- Cravo- da- Califórnia (1467. Já a acurácia seletiva da eficiência produtiva, para quatro colheitas, foi 77,4%. Para este caráter, as maiores médias genéticas (>8,0 kg.m-3 foram dos

  10. Estudio de tres niveles de fertilización química y su efecto en el comportamiento agronómico de dos híbridos de pimiento (capsicum annum l) en el sector del recinto “el limón” cantón palestina provincia del Guayas

    OpenAIRE

    Suarez Vulgarin, Jorge Guillermo; Donoso Bruque, Manuel

    2009-01-01

    El presente proyecto de tesis de grado, abarca el estudio de ocho tratamientos compuesto por dos híbridos de pimiento indeterminado con tres planes de fertilización química y un testigo. El presente proyecto de tesis de grado, se realizo durante los meses de Septiembre a Abril de los años 2005 y 2006 consecuentemente, en la Finca “Pilar”, ubicada en el recinto “El Limón” del Cantón Palestina en la Provincia del Guayas a 22 msnm, a 1 grado 38 minutos y 40 segundos de latitud sur y a los ...

  11. Produção de mudas de limão cravo em substrato com biofertilizante bovino irrigado com águas salinas Production of rangpur lemon seedlings in substrate with bovine biofertilizer irrigated with saline water

    Directory of Open Access Journals (Sweden)

    Alex Matheus Rebequi

    2009-12-01

    Full Text Available Um experimento foi instalado no período de março a maio de 2008, em ambiente telado, para avaliar os efeitos da salinidade da água de irrigação e do biofertilizante bovino na formação de mudas de limão cravo. O substrato foi material dos primeiros 20 cm de um Argissolo Amarelo Distrófico não salino, com os tratamentos dispostos em esquema fatorial 5 x 2 com seis repetições, referente a cinco valores de condutividades elétrica da água de irrigação (0,5; 1,0; 2,0; 3,0 e 4,0 dS.m-1, obtidos pela diluição de uma água fortemente salina (14,3 dS.m-1 com água não salina (0,5 dS.m-1, em solo sem e com biofertilizante comum, diluído em água na proporção de 1:1 aplicado ao nível de 10% do volume do substrato, um dia antes da semeadura. Pelos resultados, se constatou superioridade dos dados obtidos nos tratamentos com o biofertilizante em relação aos dos tratamentos sem o respectivo insumo.One experiment was developed during the period from March to May of 2008 in a canvassed shelter to evaluate the effects of saline water and bovine biofertilizer on seedling formation of rangpur lemon. The substrate used was composed by soil at 20 cm depth of a non saline yellow distrofic Oxisoil, with treatments distributed in a factorial arrangement 5 x 2 with six repetitions, referring to water electrical conductivity (0.5, 1.0, 2.0, 3.0 e 4.0 dS.m-1 obtained by dilution of saline water (14.3 dS.m-1 with non saline water (0.5 dS.m-1 in soil with and without biofertilizer diluted in water 1:1 proportion applied in 10% of substrate volume, one day before sowing. According to results, it was observed a superiority of data from treatments with biofertilizer in relation to those without this product.

  12. Multi-organ expression profiling uncovers a gene module in coronary artery disease involving transendothelial migration of leukocytes and LIM domain binding 2: The Stockholm Atherosclerosis Gene Expression (STAGE) study

    KAUST Repository

    Hägg, Sara

    2009-12-04

    Environmental exposures filtered through the genetic make-up of each individual alter the transcriptional repertoire in organs central to metabolic homeostasis, thereby affecting arterial lipid accumulation, inflammation, and the development of coronary artery disease (CAD). The primary aim of the Stockholm Atherosclerosis Gene Expression (STAGE) study was to determine whether there are functionally associated genes (rather than individual genes) important for CAD development. To this end, two-way clustering was used on 278 transcriptional profiles of liver, skeletal muscle, and visceral fat (n =66/tissue) and atherosclerotic and unaffected arterial wall (n =40/tissue) isolated from CAD patients during coronary artery bypass surgery. The first step, across all mRNA signals (n =15,042/12,621 RefSeqs/genes) in each tissue, resulted in a total of 60 tissue clusters (n= 3958 genes). In the second step (performed within tissue clusters), one atherosclerotic lesion (n =49/48) and one visceral fat (n =59) cluster segregated the patients into two groups that differed in the extent of coronary stenosis (P=0.008 and P=0.00015). The associations of these clusters with coronary atherosclerosis were validated by analyzing carotid atherosclerosis expression profiles. Remarkably, in one cluster (n =55/54) relating to carotid stenosis (P =0.04), 27 genes in the two clusters relating to coronary stenosis were confirmed (n= 16/17, P<10 -27and-30). Genes in the transendothelial migration of leukocytes (TEML) pathway were overrepresented in all three clusters, referred to as the atherosclerosis module (A-module). In a second validation step, using three independent cohorts, the Amodule was found to be genetically enriched with CAD risk by 1.8-fold (P<0.004). The transcription co-factor LIM domain binding 2 (LDB2) was identified as a potential high-hierarchy regulator of the A-module, a notion supported by subnetwork analysis, by cellular and lesion expression of LDB2, and by the

  13. Controle da ferrugem das folhas do capim-limão [Cymbopogon citratus (DC: Stapf] com produtos naturais Rust control in leaves of lemongrass [Cymbopogon citratus (DC: Stapf] by using natural products

    Directory of Open Access Journals (Sweden)

    E.R. Lorenzetti

    2012-01-01

    Full Text Available Cymbopogon citratus (DC. Stapf é uma planta medicinal pertencente a família Poaceae, perene, importante para a indústria farmacêutica e alimentícia devido ao óleo essencial que produz. Um problema limitante para a produção comercial é a ferrugem, doença cujo agente etiológico é o fungo Puccinia nakanishikii Dietel. O presente trabalho objetivou avaliar a ação de produtos naturais no controle da doença in vitro e in vivo. Para os testes in vitro, empregaram-se os seguintes produtos sobre a germinação de esporos do patógeno: óleos essenciais de eucalipto, palma-rosa, cravo-da-índia, menta, limão, citronela e mil-folhas; extrato pirolenhoso de eucalipto e mentol cristalizado, em diferentes concentrações. Para os testes de campo foram selecionados quatro produtos, o óleo de citronela e de eucalipto, o extrato pirolenhoso, e o mentol. In vitro, todos os agentes mostraram-se promissores no controle do patógeno. Quando em condições in vivo, sob alta severidade da doença no ato da avaliação, os produtos testados foram capazes de reduzir a severidade da doença em comparação com a testemunha. O controle da doença com os agentes naturais variou de 38% (extrato pirolenhoso a 61%(óleo essencial de eucalipto.Cymbopogon citratus (DC. Stapf is a medicinal plant that belongs to the Poaceae family; it is perennial and important for the pharmaceutical and food industry due to the essential oil it produces. A limiting problem for commercial production is rust, a disease that has as etiological agent the fungus Puccinia nakanishikii Dietel. The present study aimed to evaluate the action of natural products on the disease control, in vitro and in vivo. For the in vitro tests, the following products were employed on the germination of the pathogen spores: essential oils from eucalyptus, palmarosa, clove, peppermint, lemon, citronella and yarrow, pyroligneous extract from eucalyptus, and crystallized menthol, at different

  14. Efeitos de fitorreguladores e nitrato de potássio na germinação de sementes do limão 'volkameriano' Effects of growth regulators and potassium nitrate on 'volkameriana' lemon seed germination

    Directory of Open Access Journals (Sweden)

    E.O. Ono

    1993-12-01

    Full Text Available Foram estudados os efeitos de substâncias reguladoras de crescimento, bem como do nitrato de potássio (KNO3, na germinação de sementes do limão 'volkameriano' (Citrus volkameriana Pasq.. O experimento foi realizado em germinador, contendo papel de filtro umedecido com água destilada e gerbox, como meio para a germinação das sementes. Estas foram retiradas de frutas maduras, lavadas, secas à sombra e armazenadas durante 101 dias em câmara fria. Logo após, foram tratadas com os fitorreguladores e o KNO3 por 24 horas, conforme os tratamentos: H2O; 50 mg/l de GA3; 250 mg/l de GA3; 50 mg/l de GA4 + GA7 + fenilmetilaminopurina; 100 mg/l de GA4 + GA7 + fenilmetilaminopurina; 20 mg/lde fenilmetilaminopurina; 40 mg/lde fenilmetilaminopurina e 0,2% de KNO3. As avaliações foram realizadas depois de 15 dias da semeadura, sendo o intervalo de 2 dias. Através da análise estatística dos resultados obtidos, foi possível concluir que no tratamento com 50 mg/lde GA4 + GA7 + fenilmetilaminopurina houve a maior porcentagem de germinação (89,00% de sementes, num menor tempo médio (22,29 dias.The purpose of this research was to study the effects of growth regulators and potassium nitrate on 'volkameriana' lemon seed germination. The medium for seed germination was filter paper with distilled water. The seeds were removed from ripe fruits, washed, dried and stored at 4-5°C. After this the seeds were treated with growth regulators and potassium nitrate during 24 hours. The treatments were H2O; 50 mg/lof GA3; 250 mg/lof GA3; 50 mg/lof GA4 + GA7 + phenylmethylaminepurine; 100 mg/l of GA4 + GA7 + phenylmethyla-minepurine; 20 mg/lof phenylmethylaminepurine; 40 mg/lof phenylmethylaminepurine and 0,2% of KNO3. The evaluations were performed every two days, starting 15 days after seeding. The moisture with GA4 + GA7 plus phenylmethylaminepurine at 50 mg/lproved to be the most beneficial in enhancing seed germination.

  15. Multi-organ expression profiling uncovers a gene module in coronary artery disease involving transendothelial migration of leukocytes and LIM domain binding 2: the Stockholm Atherosclerosis Gene Expression (STAGE study.

    Directory of Open Access Journals (Sweden)

    Sara Hägg

    2009-12-01

    Full Text Available Environmental exposures filtered through the genetic make-up of each individual alter the transcriptional repertoire in organs central to metabolic homeostasis, thereby affecting arterial lipid accumulation, inflammation, and the development of coronary artery disease (CAD. The primary aim of the Stockholm Atherosclerosis Gene Expression (STAGE study was to determine whether there are functionally associated genes (rather than individual genes important for CAD development. To this end, two-way clustering was used on 278 transcriptional profiles of liver, skeletal muscle, and visceral fat (n = 66/tissue and atherosclerotic and unaffected arterial wall (n = 40/tissue isolated from CAD patients during coronary artery bypass surgery. The first step, across all mRNA signals (n = 15,042/12,621 RefSeqs/genes in each tissue, resulted in a total of 60 tissue clusters (n = 3958 genes. In the second step (performed within tissue clusters, one atherosclerotic lesion (n = 49/48 and one visceral fat (n = 59 cluster segregated the patients into two groups that differed in the extent of coronary stenosis (P = 0.008 and P = 0.00015. The associations of these clusters with coronary atherosclerosis were validated by analyzing carotid atherosclerosis expression profiles. Remarkably, in one cluster (n = 55/54 relating to carotid stenosis (P = 0.04, 27 genes in the two clusters relating to coronary stenosis were confirmed (n = 16/17, P<10(-27 and-30. Genes in the transendothelial migration of leukocytes (TEML pathway were overrepresented in all three clusters, referred to as the atherosclerosis module (A-module. In a second validation step, using three independent cohorts, the A-module was found to be genetically enriched with CAD risk by 1.8-fold (P<0.004. The transcription co-factor LIM domain binding 2 (LDB2 was identified as a potential high-hierarchy regulator of the A-module, a notion supported by subnetwork analysis, by cellular and lesion expression of LDB2

  16. Hospital Outpatient Prospective Payment System (OPPS) Lim...

    Data.gov (United States)

    U.S. Department of Health & Human Services — Hospital Outpatient Prospective Payment System (OPPS) Limited Data Set This file contains select claim level data and is derived from 2010 hospital outpatient PPS...

  17. Sugarcane Molasse and Whey as Additives in the Silage of Lemongrass (Cymbopogon citratus [DC.] Stapf Leaves Melaza de Caña de Azúcar y Suero de Leche como Aditivos en el Ensilaje de Hojas de Zacate de Limón (Cymbopogon citratus [DC.] Stapf

    Directory of Open Access Journals (Sweden)

    Lucía María Cristina Ventura-Canseco

    2012-03-01

    Full Text Available After extraction of essential oil from lemongrass (Cymbopogon citratus [DC.] Stapf leaves by steam distillation (heat treatment, large amounts of organic wastes are obtained. This by-product could easily be used as animal feed. The potential of sugarcane molasse and whey as additives to ensile lemongrass leaves was investigated. The vegetative material was obtained after essential oils were extracted from lemongrass leaves with steam distillation. Lemongrass leaves were chopped, mixed with sugarcane molasse and whey, placed in cylindrical plastic containers, hermetically closed. The experimental design for pH optimization was a response surface 3-level factorial design 3² with three replicates. Sugarcane molasse at 5, 10 and 15% (w/w and whey at 20, 25 and 30% (w/w were used. The pH of the silage decreased significantly in each treatment, but it was faster when sugarcane molasse was added. The lactic acid concentration was 2.8 g kg-1 DM in silage amended with 15% molasse plus 25% whey and was higher than in silage supplemented with commercial additive after 15 d. In conclusion, it was shown that lemongrass leaves obtained as by product after extracting essential oils can be effectively ensiled with whey and sugarcane molasse as additives inducing a faster production of lactic acid.Después de la extracción del aceite esencial de zacate limón (Cymbopogon citratus [DC.] Stapf por destilación con vapor (tratamiento térmico se obtiene un residuo. Este residuo podría ser utilizado para alimentación animal. El potencial de la melaza de caña y suero de leche como aditivos para ensilar hojas de zacate limón fue investigado. El material vegetal se obtuvo después que los aceites esenciales se extrajeron de las hojas de zacate limón usando destilación con vapor. Las hojas de zacate limón se picaron, mezclaron con melaza de caña y suero de leche y se colocaron en recipientes cilíndricos de plástico y se cerraron herméticamente. El dise

  18. Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral Antioxidant and fungitoxic activities of the lemongrass essential oil and citral

    Directory of Open Access Journals (Sweden)

    Luiz Gustavo de Lima Guimarães

    2011-06-01

    Full Text Available Tendo em vista a importância dos óleos essenciais, objetivou-se, com este trabalho, avaliar a atividade antioxidante e os efeitos fungitóxicos do óleo essencial de capim-limão e do seu constituinte majoritário citral sobre a inibição micelial dos fitopatógenos Fusarium oxysporum cubense, Colletotrichum gloeosporioides, Bipolaris sp. e Alternaria alternata. A atividade antioxidante foi realizada, empregando os métodos de redução do radical livre DPPH e o ensaio de oxidação do sistema b-caroteno/ácido linoléico. Para cada metodologia, utilizou-se o delineamento inteiramente casualizado em esquema fatorial de 4 x 5, sendo quatro compostos (óleo essencial, citral, timol e α-tocoferol e cinco concentrações (5; 10; 25; 50 e 100 µg L-1, com três repetições Os ensaios para verificação das atividades fungitóxicas foram realizados aplicando o teste de fumigação in vitro sobre a inibição micelial dos fungos fitopatogênicos estudados. Os tratamentos foram dispostos de forma inteiramente casualizada, com três repetições e em esquema fatorial com arranjo variável entre os fitopatógenos. O óleo essencial e o citral apresentaram pequena atividade antioxidante perante a metodologia que avalia a redução do radical estável DPPH, no entanto, perante a metodologia que emprega o sistema emulsificado β-caroteno/ácido linoléico estes compostos apresentaram atividades antioxidantes significativas. Nas atividades fungitóxicas, o óleo essencial e o citral apresentaram uma maior atividade sobre o fitopatógeno Alternaria alternata. Em relação aos valores de IC50 os menores valores apresentados pelo óleo essencial (75,83 µg mL-1 e pelo citral (58,24 µg mL-1 foram sobre o Bipolaris sp. O citral causou as maiores inibições miceliais, para todos os fitopatógenos.Given the importance of essential oils, this study evaluated the antioxidant activity and fungitoxic effects of lemongrass essential oil and its main constituent

  19. Profundidade de amostragem do solo para avaliação do enxofre disponível em pomar de limão siciliano (Citrus limon Burm. Soil sampling depth for available sulphur evaluation in sicilian lemon (Citrus limon Burm. orchard

    Directory of Open Access Journals (Sweden)

    L.I. Prochnow

    1995-04-01

    Full Text Available Em um Latossolo Vermelho Escuro, textura média, no qual foram estabelecidos diferentes teores de enxofre, através da aplicação de doses crescentes de gesso agrícola (0, 1, 2 e 4 t/ha, estudou-se a profundidade adequada para coleta de amostras de terra para avaliação do S-SO4-2 "disponível" para o limão siciliano (Citrus limon Burm. enxertado sobre porta enxerto Volkameriano. As quantidades de sulfato no solo, extraídas pelo NH4OAc 0,5 M em HOAc 0,25 M, foram determinadas aos 6, 12 e 24 meses após a aplicação do produto nas seguintes profundidades: 0-5, 5-10, 10-20, 20-40, 40-80 e 80-120 cm. As quantidades de enxofre nas folhas do limão siciliano foram também determinadas na mesma época. Os dados obtidos e os estudos de correlação que se seguiram demonstraram evidências de que o limão siciliano teria, no período considerado, extraído enxofre, principalmente, da camada de O a 20 cm de profundidade, e assim sendo, fornece indícios de que esta deve ser a camada amostrada para fins da avaliação deste importante elemento nesta cultura.On a medium-textured Dark-Red Latosol (Typic Hapludox, in which increasing levels of sulfate were established by the application of different rates of phosphogypstim (0, 1, 2 e 4 t/ha, a study was carried out to obtain information about the depth of soil samples collected in order to evaluate the S-SO4-2 availability for the Sicilian lemon (Citrus limon Burm. grafted on "Volkameriano" rootstocks. Sulfate contents at different depths (0-5, 5-10, 10-20, 20-40, 40-80 and 80-120 cm were determined, by using the NH4OAc 0,5 M in HOAc 0,25 M extractor, 6,12 and 24 months after phosphogypsum application. Sulphur content in the leaves of the lemon plants were also determined at the same dates. Analytical data obtained and the correlation studies that followed, showed that the Sicilian lemon extracted sulphur mainly from the layer 0-20 cm and, therefore, this layer is considered representative for soil

  20. Retos y desafíos para el fortalecimiento y apoyo de las capacidades de gestión empresarial a través del trabajo comunal universitario (TCU) en las PYMES de Pococí, Limón, Costa Rica

    OpenAIRE

    Sandí Delgado, Juan Carlos; Brenes Granados, Cristian

    2011-01-01

    Este artículo se centra en la elaboración de un diagnóstico cuyo fin será determinar los retos y desafíos para el fortalecimiento y apoyo de las capacidades de gestión empresarial de las organizaciones públicas y PYMES, ubicadas en el Cantón de Pococí, Provincia de Limón, Costa Rica. La investigación llevada a cabo fue de tipo cuantitativa. Se efectuó una revisión bibliográfica y los datos primarios fueron recolectados a través de la aplicación de cuestionarios con preguntas...

  1. Retos y desafíos para el fortalecimiento y apoyo de las capacidades de gestión empresarial a través del trabajo comunal universitario (tcu) en las PYMES de Pococí, Limón, Costa Rica

    OpenAIRE

    Brenes Granados, Cristian; Sandí Delgado, Juan Carlos

    2012-01-01

    Este artículo se centra en la elaboración de un diagnóstico cuyo fin será determinar los retos y desafíos para el fortalecimiento y apoyo de las capacidades de gestión empresarial de las organizaciones públicas y PYMES, ubicadas en el Cantón de Pococí, Provincia de Limón, Costa Rica. La investigación llevada a cabo fue de tipo cuantitativa. Se efectuó una revisión bibliográfica y los datos primarios fueron recolectados a través de la aplicación de cuestionarios con preguntas semi- c...

  2. Design and performance of the 10W class small scale Savonius wind powered generation system; 10W kyu kogata Savonius gata fusha hatsudenki no shisaku kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Ito, M.; Ushiyama, I. [Ashikaga Institute of Technology, Tochigi (Japan); Hagiwara, T. [Yokogawa Weathac Corp., Tokyo (Japan)

    1994-12-08

    The present study discusses the practicability of a wind power generator for a small scale power source. A wind tunnel experiment was performed using small scale Savonius wind turbines. Two kinds of wind turbines with and without end plates were used for the test to investigate the wind velocity versus output characteristics and examine their practicability. A proper load for the Savonius wind powered generation system in this experiment was found to be about 40 ohm for the turbine with end plates and about 30 ohm for the one without end plates. The output decreases under loads other than these values. The output characteristics change curvilinearly under an electric load, and linearly under a battery load. The power of the turbine without end plates decreases to about half of that with end plates at the same wind velocity. According to the output characteristics at each wind velocity setting (4, 6 and 8 m/s), the maximum power is 5.02W for the turbine with end plates and 2.67W for the one without end plates. The wind turbines were verified to have practicability as a small scale wind powered generation system. The efficiency of a DC servo motor in the tested generator was calculated as 60% from the experimental values. For the effect of using the end plates in heavy snow areas, the selection should be made according to actual situations in particular areas. 3 refs., 7 figs., 3 tabs.

  3. Development of conduction cooled 10T-Nb{sub 3}Al superconducting magnet; 10T kyu Nb{sub 3}Al dendo reikyaku magunetto no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Wadayama, Y.; Hara, N.; Azuma, K.; Suzuki, Y.; Aihara, K. [Hitachi Ltd., Tokyo (Japan)

    1999-06-07

    The development to the magnetism application field is expected conduction cooling magnet using the small G-M refrigerating machine. The operation is being convenient why, and the compactification is due to be possible. This time, production and test were done in respect of conduction cooling coil using long length modified jelly roll process Nb{sub 3}Al wire rod. Middle lamella is Nb{sub 3}Al on the coil in the three-layer structure in the internal, and the outer layer is NbTi. In this report, principal objective is reported on mischievous prank test result, etc. for basic structure of the magnet and cooling of the Nb{sub 3}Al coil and evaluation of the excitation characteristics. (NEDO)

  4. Ocorrência do vírus "vein enation (woody gall" em limão verdadeiro (Citrus limon burm. cv siciliano em Santa Catarina Occurrence of the "vein enetion (woody gall" virus in lemon (Citrus limon Burm. cv siciliano in the Santa Catarina state

    Directory of Open Access Journals (Sweden)

    O.L. KOLLER

    1998-01-01

    Full Text Available Relata-se a ocorrência do vírus causador da doença "vein enation", também conhecida por "woody gall", em plantas de limão verdadeiro, em Santa Catarina, introduzido em pomar comercial através de mudas adquiridas em São Paulo. Limão `Volkameriano' enxertado com material contaminado desenvolveu os sintomas característicos de "woody gall", com grande intensidade no Alto Vale do Itajaí, e com menor intensidade no Litoral Norte de Santa Catarina. Constatou-se redução no desenvolvimento das plantas e na produção de frutos. Todo material sabidamente contaminado foi erradicado. Sugere-se às instituições oficiais de pesquisa a realização de testes de indexação para esta virose em seus bancos de germoplasma, uma vez que grande parte das variedades cítricas não desenvolve os sintomas da doença.The presence of the vein enation virus, also known as woody gall virus, in Citrus limon, in Santa Catarina State, is reported. The virus was introduced through contamined nursery plants acquired in the São Paulo State. `Volkamer' lemon budded with contamined buds has developed typical woody gall symptoms, more frequently in Alto Vale do Itajaí (High Itajaí River Valley, and less frequently in Santa Catarina's Northern Coast. A significant decrease in plant development and fruit yield was been observed. All known contaminated material was erradicated. It is suggested that official research institutions carry out indexing tests for this virosis at their germplasm banks, since part of the citrus varieties fail to develop symptoms of this disease.

  5. Inversão da sacarose utilizando ácido cítrico e suco de limão para preparo de dieta energética de Apis mellifera Linnaeus, 1758 Inversion of the sucrose using citric acid and lemon juice for preparing energetic diet of Apis mellifera Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Deodoro Magno Brighenti

    2011-04-01

    Full Text Available Em época de escassez de néctar, pode-se fornecer ao enxame uma suplementação alimentar utilizando "açúcar invertido" que é obtido pela hidrólise da sacarose em meio ácido por aquecimento, formando uma mistura de glicose e frutose. O ácido normalmente utilizado em tal reação é o ácido cítrico, no entanto, diante da dificuldade de pequenos produtores apícolas em obterem tal produto comercialmente, uma alternativa seria substituí-lo pela utilização de suco de limões. Sendo assim, o objetivo deste trabalho foi quantificar a inversão da sacarose em dietas para operárias de Apis mellifera, empregando-se o ácido cítrico e sucos de limões. Prepararam-se 21 soluções aquosas de açúcar cristal na proporção de 100 g/100 mL, colocando-se em cada uma 1,0; 2,0; 3,0; 5,0; 10,0; 15,0 ou 20,0 mL de suco dos limões Galego [Citrus aurantifolia (C. Swingle], Tahiti [Citrus latifolia Tanaka] ou Cravo [Citrus limonia (L. Osbeck]. Além dessas, outras seis soluções aquosas de açúcar na mesma proporção foram preparadas adicionando-se 0,1; 0,16; 0,3; 0,5 e 0,7 g de ácido cítrico e uma para testemunha. Quantificou-se a inversão pelo método de Somogyi-Nelson. Para o limão Cravo foi encontrada a menor porcentagem de inversão. Em relação a adição de ácido cítrico, constatou-se que ao colocar 0,1 g, a inversão foi estimada em 12,2% enquanto que, com a adição de 0,16 g, a inversão seria de 18,8%. A quantidade máxima de ácido cítrico a ser adicionada foi estimada em 0,18 g adotando como critério o pH médio de 3,3 dos méis de abelhas africanizadas. Para os sucos dos limões Galego, Tahiti e Cravo, quando utilizados como substitutos do ácido cítrico, estimou-se a quantidade máxima a ser adicionada sendo igual a 2,1; 3,6 e 5,3 mL, respectivamente, para cada 100 g de açúcar em 100 mL de água, para o pH fixado em 3,3. Considerando-se a inversão e o pH, o suco do limão Tahiti forneceu melhores resultados.In times of

  6. Photophysical Properties of Aromatic and Antiaromatic Porphyrinoids

    Science.gov (United States)

    2011-04-14

    Chem. B, 2007, 111, 2138. 18 List of Publications 1. Large Porphyrin Squares from the Self-Assembly of meso- Triazole -Appended L-Shaped meso–meso...Linked ZnII–Triporphyrins: Synthesis and Efficient Energy Transfer  Chihiro Maeda, Pyosang Kim, Sung Cho, Jong Kang Park, Jong Min Lim, Dongho Kim...Characterization of Tri(Cyclopentaphenanthrene)-Derived Nanoparticles: A Template Free Synthesis of Hollow Colloidosomes.  Shanmugam Easwaramoorthi, Pyosang

  7. Probabilistic Systems with LimSup and LimInf Objectives

    CERN Document Server

    Chatterjee, Krishnendu

    2008-01-01

    We give polynomial-time algorithms for computing the values of Markov decision processes (MDPs) with limsup and liminf objectives. A real-valued reward is assigned to each state, and the value of an infinite path in the MDP is the limsup (resp. liminf) of all rewards along the path. The value of an MDP is the maximal expected value of an infinite path that can be achieved by resolving the decisions of the MDP. Using our result on MDPs, we show that turn-based stochastic games with limsup and liminf objectives can be solved in NP \\cap coNP.

  8. Expression of LIM mineralization protein-1 in the apical papilla and dental pulp tissues of human teeth%LIM矿化蛋白1在人牙髓及根尖牙乳头中的表达

    Institute of Scientific and Technical Information of China (English)

    陈旭; 张珍丽; 孙鲁雁; 郭艳

    2013-01-01

    Objective To examine the expression of LIM mineralization protein-1 (LMP-1) in the apical papilla and dental pulp tissues of human immature permanent teeth and to investigate the role of LMP-1 in the development and maturation of pulp-dentin complex.Methods Forty-eight healthy premolars in need of extraction for orthodontic treatment were obtained with 24 immature permanent teeth and 24 mature permanent teeth.After extraction,the apical papilla was detached from the dental pulp in the immature permanent tooth and the dental pulp of mature permanent tooth was rapidly removed.The samples were divided into 3 groups:group 1,apical papilla of immature permanent teeth (root formed 2/3 of its full length) ; group 2,dental pulp tissues of immature permanent teeth; group 3,dental pulp tissues of mature permanent teeth.There were 24 samples for each group.Half of them were used for reverse transcriptien-PCR (RT-PCR) detection,and the other half were used for Western blotting detection.Band intensities were quantified using Meta Morph software 6.2.6 and subsequently normalized by dividing the band gray value ofthe target gene by the intensity of its corresponding β-actin.Two-sample t test was used to analyze the difference of expression intensity between group 1 and group 2 as well as group 2 and group 3 with SPSS 13.0 software package.Statistical significance was established as P < 0.05.Results As indicated by RT-PCR,LMP-1 expressed in the apical papilla,dental pulp of immature permanent teeth and the dental pulp of mature permanent teeth were 0.25 ± 0.09,0.46 ± 0.24 and 0.31 ± 0.10 respectively.The expression intensity of LMP-1 mRNA in the dental pulp tissues of human immature permanent teeth were significantly higher than that in the apical papilla tissues(t =2.92) and that in the dental pulp tissues of human mature permanent teeth(t =2.31) (P < 0.05).As indicated by Western blotting,LMP-1 expressed in the three groups were 0.33 ± 0.08,0.82 ± 0.10 and 0.52 ± 0

  9. Análisis del proceso de anulaciones de adeudos de trabajadores independientes, en el Área de Inspección de la Sucursal de Limón, de la Caja Costarricense de Seguro Social para identificar cuáles son los factores que causan la cantidad de trámites administrativos, proponiendo un modelo de mejora para la Institución

    OpenAIRE

    Angulo Ríos, Maylen Ivannia

    2015-01-01

    Universidad de Costa Rica. Posgrado en Administración y Dirección de Empresas. Maestría Profesional en Administración y Dirección de Empresas, 2015. El objetivo general del trabajo es analizar el proceso de las anulaciones de adeudos a los trabajadores independientes en el Área de Inspección de la Sucursal de la Caja Costarricense de Seguro Social en Limón, para identificar los factores causantes de la cantidad de trámites administrativos, mediante la aplicación de herramientas de la inves...

  10. Korrekturverfahren zur numerischen Lösung nichtlinearer Optimierungsprobleme mittels Methoden der parametrischen Sensitivitätsanalyse

    OpenAIRE

    Nikolayzik, Tim

    2012-01-01

    The nonlinear optimization has becoming of the key technologies of the field of applied mathematics. There are various numbers of applications in different fields of science, e.g. economics, medicine and engineering. Also industry is using mathematical algorithms for solving nonlinear optimization problems. Especially in the aerospace industry nonlinear optimization is frequently used for optimizing trajectories. The aim of this thesis is to extend an existing method for solving nonlinear opt...

  11. Ökologische Autarkie als Beitrag zur Lösung unserer Zukunftsprobleme

    OpenAIRE

    Weller, Wolfgang

    2011-01-01

    Der Mensch als Gast dieser Erde hat ihr Antlitz maßgeblich verändert und dabei ihre Schätze ausgebeutet und auch die Natur geschädigt. Hier wurde inzwischen ein Zustand erreicht, der ein Umdenken erfordert. Im vorliegenden Beitrag wird ein neues Paradigma vorgestellt, welches systemtheoretisch begründet wird und auf die Prinzipien von Autarkie und Ökologie setzt. Zur Verwirklichung dieses Konzepts wird eine Vielzahl von Maßnahmen aufgeführt, deren Darlegung in übersichtlicher Weise erfolgt. ...

  12. Untersuchungen zur Bedeutung anthelminthischer Behandlungen bei der Auslösung von Symptomen einer larvalen Cyathostominose

    OpenAIRE

    Steinbach, Tanja

    2003-01-01

    Im Rahmen dieser Arbeit wird untersucht, ob bei Pferden durch die Gabe von Anthelminthika Symptome einer larvalen Cyathostominose ausgelöst werden können. Im Zentrum des Interesses stehen dabei die Anthelminthika Moxidectin und Fenbendazol, die aufgrund ihrer larviziden Wirksamkeit als Mittel der ersten Wahl für die kausale Therapie und Metaphylaxe der larvalen Cyathostominose angesehen werden. Der Versuch wird an 24 Ponyfohlen durchgeführt, die in die folgenden Gruppen unterteilt werden...

  13. Satellitensysteme zur Lösung des Problems von Asteroiden- und Kometeneinschlägen.

    Science.gov (United States)

    Gritzner, C.

    Einschläge (Impakte) von Asteroiden und Kometen größer 1 km im Durchmesser sind seltene Ereignisse, jedoch mit katastrophalen Folgen für die gesamte irdische Biosphäre. Auch kleinere erdnahe Objekte (NEOs - Near-Earth Objects) können beträchtliche direkte und sekundäre Schäden anrichten. Um solche Impakte zu vermeiden, ist es notwendig, einerseits eine Beobachtungsinfrastruktur zu entwickeln, um alle relevanten NEOs zu entdecken und mit den extrapolierten Bahnparametern eine mögliche Kollision mit der Erde vorherzusagen. Mittels eines Abwehrsystems müßte man dann den NEO auf eine ungefährliche Bahn lenken. In allen Phasen, d.h. von der Suche nach NEOs, über Planung, Test und Einsatz des Abwehrsystems, können oder müssen Satellitensysteme unterschiedlicher Art eingesetzt werden. Konzepte dieser Systeme werden in dieser Arbeit vorgestellt und die mit ihnen verbundenen Probleme diskutiert.

  14. Acoustic Features and Perceptive Cues of Songs and Dialogues in Whistled Speech: Convergences with Sung Speech

    OpenAIRE

    Meyer, Julien

    2007-01-01

    International audience; Whistled speech is a little studied local use of language shaped by several cultures of the world either for distant dialogues or for rendering traditional songs. This practice consists of an emulation of the voice thanks to a simple modulated pitch. It is therefore the result of a transformation of the vocal signal that implies simplifications in the frequency domain. The whistlers adapt their productions to the way each language combines the qualities of height perce...

  15. Leadership Change in North Korean Politics: The Succession to Kim Il Sung

    Science.gov (United States)

    1988-10-01

    became a "guiding star" as early as 1973. In 1977, he was depicted as the "future sun" and the "blessed sunshine ." He has not yet been called the "sun...34 but "future sun" and "blessed sunshine " indicate that he is the future leader, following his father. In recent years, the father and the son have been...image to be destroyed after his death. Rather, he is eager to eternalize the leader-father image through generations. It is therefore prudent to

  16. Eficacia de tres estrobilurinas para el control de mancha rojiza (Guignardia mangiferae y mancha negra (Guignardia citricarpa en frutos de limón, en Tucumán, República Argentina Efficiency of three strobilurins to control reddish spot (Guignardia mangiferae and black spot (Guignardia citricarpa in lemon fruits in Tucumán, Argentina

    Directory of Open Access Journals (Sweden)

    Gabriela M. Fogliata

    2011-06-01

    Full Text Available La Argentina lidera la producción e industrialización mundial de limón. Tucumán produce el 87% del total nacional, destinando 65% para su industrialización y 35% para su comercialización como fruta fresca, principalmente en exportaciones. Para ello, se requieren frutos de calidad y libres de enfermedades, especialmente de aquellas causadas por patógenos considerados cuarentenarios en los mercados de destino. En Tucumán, están presentes la mancha negra de los cítricos, causada por Guignardia citricarpa (Phyllosticta citricarpa, patógeno cuarentenario para importantes mercados consumidores, y en mayor prevalencia, la mancha rojiza o moteado, causada por G. mangiferae (P. capitalensis, hongo no cuarentenario. Ambas enfermedades se controlan con fungicidas cúpricos, estrobilurinas, mancozeb y bencimidazoles. Se realizaron cinco ensayos para evaluar la eficacia de tres estrobilurinas, azoxistrobina, pyraclostrobin y trifloxistrobin, para controlar mancha rojiza (campañas 2004/2005, 2005/2006 y 2006/2007 y mancha negra (2007/2008 y 2008/2009 en frutos de limón en Tucumán. Se realizaron una y dos aplicaciones de estrobilurinas con oxicloruro de cobre, en un esquema de cuatro y cinco aplicaciones de cúpricos, cada treinta días desde caída de pétalos. Se utilizaron tratamientos con oxicloruro de cobre solo o en mezcla con mancozeb, como testigos químicos. Se evaluó la incidencia de las enfermedades en julio y se calculó la eficacia de los tratamientos. Los más eficaces para controlar mancha negra y mancha rojiza fueron los que incluyeron una o dos aplicaciones de estrobilurinas, sin diferencias entre sí, superando al cúprico e igualando o superando a la mezcla cúprico-mancozeb. Estos resultados demostraron la eficacia de las estrobilurinas para controlar mancha negra y mancha rojiza en frutos de limón.Argentina leads lemon world production and industrialization. The province of Tucumán generates 87% of national production

  17. Drying of sicilian lemon residue: influence of process variables on the evaluation of the dietary fiber produced Secagem do resíduo de limão siciliano: influência das variáveis de processo na avaliação da fibra alimentar produzida

    Directory of Open Access Journals (Sweden)

    Vanessa Martins Silva

    2010-06-01

    Full Text Available In this study, the effect of the process variables of the air-drying of Sicilian lemon residues on some technological properties of the fibers produced was studied. The determination and modeling of desorption isotherms were used to establish the equilibrium moisture content at 60, 75, and 90 °C using the static method with 8 saturated salt solutions. The best fit was obtained with BET and GAB models. The drying process was conducted in a vertical tray dryer and delineated according to a central composite experimental design (2² using the following as factors: air velocity (0.5, 0.75 and 1 m/s and temperature (60, 75, and 90 °C, and it presented a good fit to the exponential model (R² > 99.9%. The experimental design responses evaluated were the technological properties of the fibers: water-holding, oil-holding, and swelling capacity. Since these properties were present in high levels, the lemon residues could be used to increase content of fibers in foods resulting in the addition of nutritional benefits for the consumers.Estudou-se o efeito das variáveis de processo da secagem do resíduo de limão siciliano sobre algumas propriedades tecnológicas das fibras produzidas. A determinação e modelagem das isotermas de dessorção foram utilizadas para determinar o conteúdo de umidade de equilíbrio a 60, 75 e 90 °C, através do método gravimétrico estático com 8 soluções salinas saturadas. O melhor ajuste dos dados experimentais foi encontrado com os modelos de BET e GAB. A secagem foi conduzida em um secador vertical de bandejas e delineada de acordo com um planejamento experimental (2², tendo como fatores: velocidade do ar (0,5, 0,75 e 1 m/s e temperatura (60, 75 e 90 °C, apresentando bons ajustes ao modelo exponencial (R² > 99.9%. As respostas do planejamento avaliadas foram relacionadas a algumas propriedades tecnológicas das fibras, como a capacidade de absorção em água e óleo e o volume de intumescimento. Uma vez que

  18. Caracterização da composição química e do rendimento dos resíduos industriais do limão Tahiti (Citrus latifolia Tanaka Chemical composition and Tahiti lime industrial residues output characterization

    Directory of Open Access Journals (Sweden)

    Luciana Maria Vieira Lopes Mendonça

    2006-12-01

    Full Text Available A indústria de sucos utiliza apenas de 40% a 50% dos frutos, sendo o restante considerado resíduo industrial. Estes resíduos apresentam-se ricos em alguns componentes como pectina, vitamina C e fibras dietárias que o tornam matéria-prima para a indústria alimentícia, farmacêutica e de rações. Visando o aproveitamento dos resíduos industriais do limão Tahiti para tais fins, caracterizou-se a composição centesimal, os teores de fibras, de pectina e de vitamina C presentes nas frações flavedo, albedo e bagaço do fruto. A partir da avaliação dos dados pelo teste de Scott Knott 5% de probabilidade, observou-se que os teores de cinzas, pectina, proteína, vitamina C e fibra bruta foram diferentes no flavedo, albedo e bagaço. O resíduo industrial do limão Tahiti tem um grande potencial para uso em formulação de rações para animais, dada a sua composição química, assim como poderá ser usado para a extração de fibras, pectina, vitamina C e óleos.The juice industry makes use of only 40 to 50% of the fruit and its remainder is considered industrial residue. This residue is very rich in some of its compounds such as pectin, ascorbic acid and dietary fibers making it into raw material for the food industry, pharmaceutics and animal food. With the purpose of using the industrial residue of Tahiti lime for such purposes, the centesimal composition, fiber contents, pectin and vitamin C contained in flavedo fractions, albedo and fruit pulp were characterized. From the evaluation for the Scott Knott's test to 5% of probability, it was observed that the ash content, pectin, protein, vitamin C and rude fiber were different for flavedo, albedo and pulp. Tahiti lime industrial residue has a great potential for animal food formulation, considering its chemical composition, as well as the fact that it can be used for fiber extraction, pectin, vitamin C and oils.

  19. The beneficial effects of honeybee-venom serum on facial wrinkles in humans

    Directory of Open Access Journals (Sweden)

    Han SM

    2015-10-01

    Full Text Available Sang Mi Han,1 In Phyo Hong,1 Soon Ok Woo,1 Sung Nam Chun,2 Kwan Kyu Park,3 Young Mee Nicholls,4 Sok Cheon Pak5 1Department of Agricultural Biology, National Academy of Agricultural Science, Wanju, 2Dong Sung Pharmaceuticals Co Ltd, Seoul, 3Department of Pathology, School of Medicine, Catholic University of Daegu, Daegu, South Korea; 4Manuka Doctor Ltd, Auckland, New Zealand; 5School of Biomedical Sciences, Charles Sturt University, Bathurst, NSW, Australia Abstract: Facial wrinkles are an undesirable outcome caused by extrinsic photodamage and intrinsic aging processes. Currently, no effective strategies are known to prevent facial wrinkles. We assessed the beneficial effects of bee-venom serum on the clinical signs of aging skin. Our results show that bee-venom serum treatment clinically improved facial wrinkles by decreasing total wrinkle area, total wrinkle count, and average wrinkle depth. Therefore, bee-venom serum may be effective for the improvement of skin wrinkles. Keywords: bee venom, wrinkle, area, count, depth

  20. Efeito alelopático de sabugueiro e capim-limão na germinação de picão-preto e soja = Allelophatic effect of Sambucus australis Cham. and Schltdl. and Cymbopogon citratus (DC Stapf. in the germination of Bidens pilosa L. and soybeans

    Directory of Open Access Journals (Sweden)

    Andréa Maria Teixeira Fortes

    2009-04-01

    Full Text Available As plantas invasoras são constantes e diminuem a produtividade das culturas por competirem por espaço, nutrientes e água. Dessa forma, os agricultores adotam, em grande escala, produtos químicos eficientes no controle da lavoura e com alta toxicidade ao meioambiente. Existem, no entanto, formas alternativas para o controle de invasoras, por meio de aleloquímicos presentes em algumas plantas, dentre elas, as medicinais. Este trabalho tem como objetivo analisar as propriedades alelopáticas dos extratos das plantas medicinais Cymbopogon citratus (DC Stapf. e Sambucus australis Cham. and Schltdl. em inibir a germinação de Bidens pilosa L., sem interferir na germinação de Glycine max L. Merrill. Os extratos foram obtidos triturandose 200 g de folhas com 1 L de água destilada. As sementes foram mantidas em B.O.D. à temperatura de 25°C, com fotoperíodo de 12h de luz. Nas condições em que foram realizados os experimentos, constatou-se que o extrato de capim-limão inibiu a germinação de picão-preto sem que este inibisse a germinação da soja, enquanto o extrato de sabugueiro inibiu a germinação de picão-preto e a germinação da soja. Assim, indica-se a utilização do capim-limão, como um herbicida natural para o picão-preto.Agriculture has been suffering adaptations throughout time, from hard hand labor to the most advanced sowing and harvesting techniques. Invasive plants are a constant, diminishing productivity by competing for space, nutrients, and water. Therefore, farmers have adopted, on alarge scale, the use of efficient synthetic chemicals, which are highly toxic to the environment, in order to control plant production. However, there are other alternative means to control those competitors: using allelochemicals present in some plants, such as medicinal ones. The objective of this paper is to analyze allelophatic properties of the medicinal plants Cymbopogon citratus (DC Stapf. and Sambucus australis Cham. and Schltdl

  1. In vitro antimicrobial activity of essential oils from oregano, garlic, clove and lemon against pathogenic bacteria isolated from Anomalocardia brasilianaAtividade antimicrobiana in vitro dos óleos essenciais de orégano, alho, cravo e limão sobre bactérias patogênicas isoladas de vôngole

    Directory of Open Access Journals (Sweden)

    Tânia Fraga Barros

    2011-10-01

    Full Text Available Natural preservative substances are an efficient and economic alternative, because the aromatical plants and rich spices in essential oils characterized by a notable are gotten from antimicrobial activity, and for this reason, its derived products can be used to delay or to inhibit the growth of microorganisms. The objective of this study was to evaluate the antibacterial activity of essential oils of clove, limon, oregano (Origanum vulgare ssp. Hirtum and garlic several species of bacteria like Staphylococcus aureus and Escherichia coli isolated of vongole (Anomalocardia Brasiliana and bacteria standard ATCC (American Type Culture Collection: Escherichia coli, Staphylococcus aureus and Salmonella cholerasuis, through diffusion test and after it was determinate the Minimum Inhibitory Concentration (MIC. In this study it was observed that the garlic essential oil presented antibacterial activity front the S. aureus and the S. cholerasuis.; already the oils of clove and oregano (Origanum vulgare ssp. Hirtum had presented activity front to all the analyzed bacteria, however the oregano oil presented the bigger bacterial inhibition, 26.7mm e 29,3mm for E. coli e S. aureus respectively. The essential oil of lemon did not demonstrate antibacterial activity. In such a way the oregano and clove essential oils that they had presented antibacterial activity appeared to be the most preferable agent for future research.Antimicrobianos de origem natural são alternativas eficazes e econômicas, pois são obtidos a partir das plantas aromáticas e especiarias ricas em óleos essenciais caracterizados por uma notável atividade antimicrobiana, e por esta razão, seus produtos derivados podem ser usados para retardar ou inibir a multiplicação microbiana. O objetivo deste estudo foi avaliar a atividade antibacteriana dos óleos essenciais de cravo, limão, orégano e alho sobre as cepas de Staphylococcus aureus e Escherichia coli isolados do v

  2. 78 FR 17992 - Additional Designation of Three North Korean Individuals Pursuant to Executive Order 13382

    Science.gov (United States)

    2013-03-25

    ... of State. ACTION: Designation of Pak To-Chun, Chu Kyu-Chang, and O Kuk-Ryol Pursuant to E.O. 13382... with the Secretary of the Treasury and the Attorney General, has determined that Pak To-Chun, Chu Kyu..., Chagang Province, DPRK CHU KYU-CHANG A.K.A.: Chu Kyu-Ch'ang A.K.A.: Ju Kyu-Chang D.O.B.: November 25,...

  3. Secagem e fragmentação da matéria seca no rendimento e composição do óleo essencial de capim-limão Yield and composition of essential oil of lemongrass in different drying and fragmentation conditions

    Directory of Open Access Journals (Sweden)

    Larissa C. do B. Costa

    2005-12-01

    Full Text Available Este estudo foi conduzido com objetivo de determinar o tipo de secagem e a fragmentação das folhas de capim-limão para otimizar o rendimento extrativo do óleo essencial. Foram estabelecidos 6 tratamentos com 2 tipos de secagem (estufa de ventilação forçada a 40ºC e sala com desumidificador e 3 tamanhos de fragmentos das folhas secas (pulverização em moinho, fragmentos com 1 e com 20 cm de comprimento, com 4 repetições. O óleo essencial foi extraído por hidrodestilação durante 2 horas. O maior rendimento de óleo essencial foi obtido com o material seco na sala com desumidificador, não havendo diferenças significativas para os tamanhos da folha. O componente mais abundante no óleo essencial foi o citral que também apresentou as maiores concentrações nas folhas secas em desumidificador.In this study the drying and fragmentation conditions of lemongrass leaves were determined, to increase the essential oil yield. Four replications of six treatments were studied with 2 drying methodologies (oven-drying at 40ºC and room temperature using moisture dryer and 3 fragmentation sizes (powder obtained in mill, 1 cm and 20 cm fragments. The essential oil was extracted in Clevenger's modified apparatus during 2 hours. The higher essential oil yield and citral content was obtained with the leaves dried under room temperature using moisture dryer, with no significant differences in the fragmentation sizes.

  4. Determinação da qualidade microbiológica e físico-química de chás de Cymbopogon citratus (D.C Stapf (capim-limão = Evaluation of the microbiological and physical-chemical qualities of lemongrass tea

    Directory of Open Access Journals (Sweden)

    Eliane Carneiro Gomes

    2008-01-01

    Full Text Available Em função do grande volume de produção e comercialização do capim-limão, estima-se que uma contaminação microbiológica deste produto possa representar importante risco para a saúde pública. Esta pesquisa visou avaliar marcas de chá de capimlimão, em relação às qualidades microbiológica e físico-química, tendo como base alegislação brasileira do Ministério da Saúde. Nenhuma das amostras apresentou Salmonella sp., porém em 50% delas, houve presença de coliformes a 35ºC. Em três amostras, evidenciaram-se coliformes a 45ºC, com presença de Escherichia coli em uma destas. 81,25%das amostras apresentaram bolores e leveduras, porém sem relação direta com os teores de umidade observados. Dentre os fungos presentes, identificou-se Aspergillus niger, no entanto sem produção de aflatoxinas. Todos infusos analisados negativaram a contaminação evidenciada nos testes com o produto seco. Os resultados indicaram possíveis falhas nos procedimentos pós-colheita e de comercialização. A implementação do Sistema APPCC (HACCP poderia substancialmente minimizar esta contaminação.Considering the great volume of lemongrass production andcommercialization, it is estimated that a potential microbiological contamination of this product could represent an important hazard to public health. This study was performed to evaluate different brands of lemongrass tea, regarding their microbiological and physicalchemical quality, based on the legislation of the Brazilian Health Ministry. No traces of Salmonella sp. were found, but in 50% of samples, the presence of coliforms was detected at 35ºC. Three samples presented coliforms at 45ºC, with Escherichia coli found in one of them. 81.25% of samples presented molds and yeasts. Among the fungi present, Aspergillus niger was identified, but aflatoxins were absent. None of the infusions analyzed presented the contamination evidenced in the dry product. The results obtained may be

  5. Avaliação in vitro da ação do óleo essencial de capim limão (Cymbopogon citratus sobre o carrapato bovino Rhipicephalus (Boophilus microplus In vitro evaluation of the action of lemon grass (Cymbopogon citratus essential oil on the cattle tick Rhipicephalus (Boophilus microplus

    Directory of Open Access Journals (Sweden)

    F.C.C. Santos

    2012-01-01

    Full Text Available O uso indiscriminado de produtos químicos no controle do carrapato bovino constitui a principal causa do gradativo aumento do número de cepas resistentes deste parasita às bases disponíveis no mercado. A utilização de óleos essenciais e extratos vegetais é uma prática antiga no controle de carrapatos, porém só recentemente tem recebido a devida atenção dos pesquisadores. O objetivo deste experimento foi avaliar a eficácia in vitro do óleo de capim limão (Cymbopogon citratus sobre fêmeas ingurgitadas de Rhipicephalus (Boophilus microplus através do exame de biocarrapaticidograma. Foram testadas seis diluições do óleo de C. citratus (1; 5; 10; 25; 50 e 100% em uma população de carrapatos resistentes a amidínicos e piretróides sintéticos. A inibição de postura foi de 3; 23; 46; 66; 46 e 46%, a eclosão larval foi de 83; 58; 31; 0; 38 e 25% e a eficácia do tratamento foi de 32; 64; 83; 100; 88 e 82%, respectivamente. O óleo de C. citratus apresentou controle parcial do carrapato R. microplus in vitro, mesmo frente a populações resistentes a produtos químicos.The indiscriminate use of chemical products to control the cattle tick is the main cause of the gradual increase in the number of strains of this parasite that are resistant to the bases currently available in the market. The use of essential oils and plant extracts is an ancient practice for tick control; however, only recently has it received due attention by researchers. The aim of this experiment was to evaluate the in vitro efficacy of lemongrass (Cymbopogon citratus essential oil on engorged females of Rhipicephalus (Boophilus microplus through immersion test. Six concentrations of Cymbopogon citratus oil (1; 5; 10; 25; 50 and 100% were tested against a tick population resistant to synthetic formamidines and pyrethroids. The inhibition of egg-laying was 3; 23; 46; 66; 46 and 46%, the hatching was 83; 58; 31; 0; 38 and 25%, and the treatment efficacy was 32

  6. Influência do intervalo entre cortes sobre a produção de biomassa de duas espécies de capim limão Influence of the interval between cuts on biomass yield of two lemon grass species

    Directory of Open Access Journals (Sweden)

    André May

    2008-09-01

    Full Text Available Foi estudada a influência do intervalo entre cortes na produção de massa seca da parte aérea de duas espécies de capim limão. O experimento foi realizado no Instituto Agronômico, em Campinas-SP, 04 de junho/05 a 28 de agosto/06. O delineamento experimental utilizado foi em blocos ao acaso, com três repetições, com os tratamentos em esquema fatorial 2 x 4, sendo duas espécies (C. citratus e C. flexuosus e quatro intervalos entre cortes (40; 60; 80 e 100 dias. Para C. flexuosus, maiores intervalos entre cortes proporcionaram maior massa seca acumulada ao longo do ciclo de cultivo, partindo de 329,04 para 704,16 g planta-1 de massa seca acumulada da parte aérea, para intervalos entre cortes de 40 e 100 dias, respectivamente. A espécie C. citratus apresentou resposta linear decrescente da massa seca acumulada da parte aérea quanto maior o intervalo entre cortes utilizado, produzindo 238,68 g planta-1 utilizando intervalo entre cortes de 40 dias.The aim of this study was to analyze the influence of the interval between cuts on the dry mass yield of the aerial part of two lemon grass species. The experiment was conducted at Agronomical Institute (IAC, in Campinas - SP, from June 4th, 2005 through August 28th, 2006. The experiment was carried out in a completely randomized block design, with three replications, and the treatments in a 2 x 4 factorial design, corresponding two species (C. citratus and C. flexuosus and four intervals between cuts (40; 60; 80 and 100 days. For C. flexuosus, longer intervals between cuts generated more dry mass accumulated throughout the cultivation cycle, beginning on 329,04 to 704,16 g plant-1 of accumulated dry mass of the aerial part, for intervals between cuts of 40 and 100 days, respectively. The C. citratus species showed a decrease in its linear response for the accumulated dry mass of the aerial part the longer the interval between cuts was, yielding 238,68 g plant-1 for a 40 day interval between

  7. Modeling and Simulation of Mold Filling with LIMS

    Science.gov (United States)

    2007-11-02

    reservedSimacek ONR Workshop - 13 Flexible Tekscan sensor Handle Serial interface box Force sensitive layer (changes resistance) Cover layers...2003 University of Delaware All rights reservedSimacek ONR Workshop - 24 Auto_RTM Experiment Simulation selected time Experimental Validation of TekScan ... Tekscan ™ F Visual (Camera) Resin Flat copper strips Time V ol ta ge 2 July 2003© 2003 University of Delaware All rights reservedSimacek ONR Workshop

  8. Plant growth and phosphorus uptake in mycorrhizal rangpur lime seedlings under different levels of phosphorus Crescimento e absorção de fósforo por plântulas de limão 'Cravo' micorrizadas sob diferentes níveis de fósforo

    Directory of Open Access Journals (Sweden)

    Marco Antonio Nogueira

    2006-01-01

    Full Text Available The objective of this work was to evaluate the response of rangpur lime (Citrus limonia to arbuscular mycorrhiza (Glomus intraradices, under P levels ranging from low to excessive. Plants were grown in three levels of soluble P (25, 200 and 1,000 mg kg-1, either inoculated with Glomus intraradices or left noninoculated, evaluated at 30, 60, 90, 120 and 150 days after transplanting (DAT. Total dry weight, shoot P concentration and specific P uptake by roots increased in mycorrhizal plants with the doses of 25 and 200 mg kg-1 P at 90 DAT. With 1,000 mg kg-1 P, mycorrhizal plants had a transient growth depression at 90 and 120 DAT, and nonmycorrhizal effects on P uptake at any harvesting period. Root colonization and total external mycelium correlated positively with shoot P concentration and total dry weight at the two lowest P levels. Although the highest P level decreased root colonization, it did not affect total external mycelium to the same extent. As a result, a P availability imbalance affected negatively the mycorrhizal symbiosis and, consequently, the plant growth.O objetivo deste trabalho foi avaliar a resposta do limão 'Cravo' (Citrus limonia à micorriza arbuscular (Glomus intraradices, com variações de níveis de P de baixo a excessivo. As plantas foram cultivadas em três níveis de P solúvel (25, 200 e 1.000 mg kg-1, com inoculação de Glomus intraradices ou sem inoculação, e avaliadas aos 30, 60, 90, 120 e 150 dias depois do transplantio (DAT. A biomassa seca total, a concentração de P na parte aérea, e a absorção específica de P pelas raízes aumentaram nas plantas micorrizadas nas doses de 25 e 200 mg kg-1 de P aos 90 DAT. Na dose de 1.000 mg kg-1 de P, houve depressão transiente de crescimento nas plantas micorrizadas, aos 90 e 120 DAT, e não houve efeito micorrízico sobre a absorção de P em qualquer época de colheita. A colonização radicular e o micélio externo total correlacionaram-se positivamente com a

  9. Qualidade de produtos a base de plantas medicinais comercializados no Brasil: castanha-da-índia (Aesculus hippocastanum L., capim-limão (Cymbopogon citratus (DC. Stapf e centela (Centella asiatica (L. Urban Quality of products made from medicinal plants commercialized in Brazil: horsechestnut (Aesculus hippocastanum L., lemongrass (Cymbopogon citratus (DC. Stapf, and gotu kola (Centella asiatica (L. Urban

    Directory of Open Access Journals (Sweden)

    Joabe Gomes de Melo

    2007-03-01

    Full Text Available Com o objetivo de avaliar a qualidade de produtos a base de plantas medicinais, analisou-se dez amostras de castanha-da-índia, onze de capim-limão e seis de centela, comercializadas em farmácias da cidade do Recife, provenientes de indústrias de várias partes do país. Analisamos as informações técnico-científicas nos rótulos e/ou bulas de acordo com as exigências e obrigatoriedades da legislação brasileira (RDC n. 140 de 29/05/2003 e na RDC n. 102 de 30/11/2000, e embasamento da literatura científica. Também avaliou-se as características organolépticas, a autenticidade e a pureza dos produtos. Constatou-se ausência das informações obrigatórias em 92,59% das amostras. Dentre os termos vedados, foi comum a sugestão da inexistência de efeitos colaterais ou adversos. Quanto as indicações terapêuticas não se observou padronização técnica dos termos nem comprovação científica para algumas das atividades atribuídas pelos fabricantes. Todas as amostras foram consideradas autênticas com base nos testes fitoquímicos e farmacobotânicos. Na análise da pureza 59,26% das amostras foram reprovadas, devido principalmente ao elevado teor de umidade e cinzas totais. Constata-se que além dos produtos comercializados carecerem de informações e qualidade adequadas, há necessidade urgente de uma fiscalização efetiva por parte dos órgãos competentes.Aiming to evaluate the quality of products made from medicinal plants, there were analyzed ten samples of horsechestnut, eleven samples of lemongrass, and six samples of gotu kola commercialized in pharmacies from the city of Recife (Pernambuco, Brazil, coming from industries of several parts of the country. We analyzed the technical-scientific information on the labels and/or directions according to the requirements and obligations of Brazilian law (laws "RDC 140 of 29/05/2003" and "RDC 102 of 30/11/2000" and to scientific literature embasement. The products' organoleptic

  10. Problemes de traduction dans le domaine de la poesie chantee (Problems of Translation in the Area of Sung Poetry)

    Science.gov (United States)

    Grandmont, Suzanne de

    1978-01-01

    In the translation of songs, to the usual problems of rendering meaning, symbols and imagery are added the exigencies of cadence, rhythm, tempo and tonic accent. The process is illustrated by an account of the rendering in French of the musical comedy, "The Fantasticks." (Text is in French.) (AMH)

  11. Umwandlung von Kohlendioxid mit Übergangsmetall-Homogenkatalysatoren: eine molekulare Lösung für ein globales Problem?

    KAUST Repository

    Cokoja, Mirza

    2011-08-29

    A plethora of methods have been developed over the years so that carbon dioxide can be used as a reactant in organic synthesis. Given the abundance of this compound, its utilization in synthetic chemistry, particularly on an industrial scale, is still at a rather low level. In the last 35 years, considerable research has been performed to find catalytic routes to transform CO2 into carboxylic acids, esters, lactones, and polymers in an economic way. This Review presents an overview of the available homogeneous catalytic routes that use carbon dioxide as a C1 carbon source for the synthesis of industrial products as well as fine chemicals.

  12. Konflikt und Konfliktlösung in Guatemala – Die Verwirklichung der Rechte indigener Frauen im rechtspluralistischen Raum

    NARCIS (Netherlands)

    A. Titze (Anja)

    2008-01-01

    textabstractIm 20. Jahrhundert erlebte die Menschheit die Gräueltaten von zwei Weltkriegen und zahllosen Bürgerkriegen, die allesamt unermessliches Leid brachten. Auch Guatemala ist insoweit ein trauriges Beispiel. Der Bürgerkrieg, der dort in der Zeit des Kalten Krieges wütete und erst 1996 endete,

  13. Welche Bodenzusätze helfen bei der Lösung von Problemen mit Feldaufgang in Spinat?

    OpenAIRE

    Meyer, Audrey; Fuchs, Jacques G.; Schärer, Hansjakob

    2013-01-01

    Damping-off of spinach, caused by soil-borne pathogens, is one of the most serious problems in organic spinach production. To overcome these problems soil amendments based on specific strains of micro-organisms or based on organic material are proposed. In pot experiments under controlled conditions 14 microbial strains and six organic soil amendments were tested on their ability to suppress soil borne diseases and to improve plant stand establishment. In a subsequent field experiment with th...

  14. Development of hydrothermal power generation plant. Development of binary cycle power generation plant (development of 10 MW-class plant); 1995 nendo nessui riyo hatsuden plant nado kaihatsu binary cycle hatsuden plant no kaihatsu. 10MW kyu plant no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    A 10 MW-class binary cycle power generation plant has been developed using a down hole pump (DHP) which exchanges the hydrothermal energy with secondary medium in the heat exchanger. For constructing the plant at Kuju-machi, Oita Prefecture, site preparation works, foundation of cooling tower, reconstruction of roads, and survey on environmental influences were conducted. To investigate installation and removal methods of DHP, a geothermal water pump-up system, current status of the binary cycle power generating system in the USA was surveyed. In this survey, a trailer mounting handling machine was inspected. Based on the survey results, a simple assembled, easy-installation type handling equipment was designed. In addition, the replacement work for motor connector joint of DHP and the strength of coil end were improved. Construction and method allowing reuse of the motor cable were considered by improving the cable and cable end portion. The air tight soundness of incoloy corrugate sheath was confirmed. Finally, a reproduction system for waste oil of DHP bearing oil was investigated. 106 figs., 52 tabs.

  15. Planar contraction flow of diluted polymer solution. ; Experiment and numerical simulation considering inertia force. Kishaku kobunshi suiyoeki ni yoru kyu shukusho nagare. ; Kansei ko wo koryoshita suchi kaiseki to kashika jikken

    Energy Technology Data Exchange (ETDEWEB)

    Kawabata, N.; Tachibana, M.; Yoshida, K. (Fukui Univ. (Japan). Faculty of Engineering); Fujita, K. (Fukui National College of Technology, Fukui (Japan)); Kimura, K. (Fukui Univ., Fukui (Japan). Graduate School)

    1992-11-25

    Studies on viscoelastic fluid flows have been carried out while focusing on cases of high viscosity fluid such as polymer solutions so as to meet industrial demand. In a viscoelastic fluid flow, inertia force, viscous force, and elastic force act on the flow field. As the velocity of the flow increases, the effects of the viscous force outgrow the others. The authors showed that stable calculations are possible even in a region where the inertia and elastic forces become important by applying Lax method to solving the constitutive equation. However, the Lax method has a drawback in that numerical viscosity is high. In this study, the CIP method is applied to solving the constitutive equation, and a comparison with respect to the two-dimensional planar contraction flow was made between the results from numerical calculation by means of this method and the results of visualizing experimentations using polymer solutions of relatively low concentration. As a result, it was confirmed by the visualizing experimentations that a flow pattern which is inherent in the viscoelastic fluid and which does not appear in the case of high viscosity fluid. Furthermore this characteristic pattern was also obtained by numerical calculations by this method. 9 refs., 10 figs.

  16. 新型人工生物活性接骨板治疗犬股骨骨折%LIM mineralization protein 1 gene transfected bone marrow mesenchymal stem cells combined with nano-hydroxyapatite/polyamide 66 plate in the treatment of canine femoral fractures

    Institute of Scientific and Technical Information of China (English)

    徐小平; 倪卫东; 高仕长; 蒲超; 黄伟弘

    2012-01-01

    BACKGROUND: Studies have shown that nano-hydroxyapatite/polyamide 66 (n-HA/PA66) is a kind of high-strength and high-toughness composite material with excellent biocompatibility, biological safety, biological activity and bone conduction ability.OBJECTIVE: To investigate the effect and feasibility of n-HA /PA66 plate combined with bone marrow mesenchymal cells (BMSCs) transfected with human LIM mineralization protein 1 (LMP-1) gene for treatment of canine femoral fractures. METHODS: Canine BMSCs were isolated and cultured with density gradient centifugation method and adhesive culture method. The third-generation BMSCs were transfected with Adv-hLMP-1, and then combined with n-HA /PA66 plate. The dog models of femoral fractures were established in 48 dogs and were divided into four groups: Ad-LMP-1 transfection group, non-transfected group, n-HA/PA66 alone group and plate and screw groups.RESULTS AND CONCLUSION: The failure rate in the transfection group was lower than that in non-transfected group and n-HA/ PA66 plate group at 8 or 12 weeks postoperatively (P 0.05). The healing time of the transfection group was shorter than that of the other three groups. The plate in the transfection group was completely fused with canine femoral lateral cortex after 12 weeks of operation, and there was obvious bone formation between material and bone. The plate and canine lateral femoral cortex were only partially fused in the non-transfected group and n-HA/PA66 plate group, and there was no or a small amount of bone tissue formation between material and bone. The treatment of canine femoral fractures with n-HA/PA66 plate combined with BMSCs transfected with LMP-1 gene can get good fixation effects, promote the fracture healing and fusion with autogenous bone, without the need for a second operation to take out, but the strength of materials have some limitations, which must be combined with external fixation in the treatment of canine femoral fractures.%背景:研究表明纳

  17. 人LIM矿化蛋白1基因腺病毒重组表达载体构建及在犬骨髓间充质干细胞中的表达%Construction of human LIM mineralization protein-1 gene recombinant adenovirus and its expression in dog bone marrow mesenchymal stem cells

    Institute of Scientific and Technical Information of China (English)

    蒲超; 倪卫东; 高仕长; 邱宇

    2011-01-01

    背景:研究表明LIM矿化蛋白1在体内和体外都可促使骨发生.目的:应用Adeasy-1腺病毒系统构建含人LIM矿化蛋白1基因的腺病毒重组体,并感染犬骨髓间充质干细胞,检测其体外表达及诱导成骨作用.方法:构建重组腺病毒质粒pAd-LMP-1,经人胚胎肾293细胞包装、扩增后得到复制缺陷重组腺病毒Ad-LMP-1,以最佳感染复数值体外感染犬骨髓间充质干细胞,行RT-PCR及 Western Blot检测LIM矿化蛋白1、骨形态发生蛋白7基因的表达.重组Ad-LMP-1和(或)外源性重组人骨形态发生蛋白7处理犬骨髓间充质干细胞,21 d后行矿化(钙)结节茜素红染色分析LIM矿化蛋白1基因的诱导成骨作用.结果与结论:①将Ad-LMP-1以感染复数值100感染犬骨髓间充质干细胞可获得最佳感染效率,感染后骨髓间充质干细胞能在基因和蛋白水平表达LIM矿化蛋白1,未引起骨形态发生蛋白7基因的表达.②Ad-LMP-1或重组人骨形态发生蛋白7均不能单独促使骨髓间充质干细胞向成骨细胞转化,两者联合可促使骨髓间充质干细胞向成骨细胞转化.③实验成功构建重组腺病毒载体Ad-LMP-1,并实现其在犬骨髓间充质干细胞中表达,证实了LIM矿化蛋白1在诱导成骨作用中表现为与外源性重组人骨形态发生蛋白7的协同效应.%BACKGROUND: Studies shows that the LIM mineralization protein 1 (LMP-1) can stimulate bone formation in vivo and in vitro.OBJECTIVE: To construct the recombinant adenovirus vector containing LMP-1 gene by using the Ad-Easy system, then to detect the gene expression and study the mechanism of bone formation induced by LMP-1 in infected dog bone marrow mesenchymal stem cells (BMSCs).METHODS: The recombinant plasmid pAd-LMP-1 was constructed. The replication-defective recombinant adenovirus Ad-LMP-1was packaged and amplified in the human embryonic kidney 293 cells (HEK293). Dog BMSCs were infected by Ad-LMP-1 in the best value of MOI in

  18. TA'LIM AL-LUGHAH 'ABR AL-TSAQAFAT WA TATHBIQUHU FI TA'LIM AL-LUGHAH AL-'ARABIYAH FI INDONESIA

    Directory of Open Access Journals (Sweden)

    Nasaruddin

    2015-12-01

    Full Text Available This article examines the close relationship that exists between language and culture which reflected in that language is a pot of culture and culture is a frame of language usage. It follows from this relationship that language learning is the linguistic and cultural process, or what so-called teaching language across cultures. This cultural teaching of language is based on three theories first of which is “Contrastive Analysis”, “Learning The Language Is A Cultural Learning”, and “Cultural Awareness In Foreign Language Teaching And Learning”. Based on these three theories this article tries to put down a cultural pattern for the teaching of Arabic for Indonesian learners which has cultural goals and content. It aims at overcoming linguistic and cultural problems suffered by the Indonesians when learning the Arabic, especially their difficulty in understanding Arabic elements that are different from what in their language, and their difficulty in using Arabic in certain situations properly.

  19. TA'LIM AL-LUGHAH 'ABR AL-TSAQAFAT WA TATHBIQUHU FI TA'LIM AL-LUGHAH AL-'ARABIYAH FI INDONESIA

    Directory of Open Access Journals (Sweden)

    Nasaruddin Nasaruddin

    2015-12-01

    Full Text Available This article examines the close relationship that exists between language and culture which  reflected in that language is a pot of culture and culture is a frame of language usage. It follows from this relationship that language learning is the linguistic and cultural process, or what so-called teaching language across cultures. This cultural teaching of language is based on three theories first of which is “Contrastive Analysis”, “Learning The Language Is A Cultural Learning”, and “Cultural Awareness In Foreign Language Teaching And Learning”. Based on these three theories this article tries to put down a cultural pattern for the teaching of Arabic for Indonesian learners which has cultural goals and content.  It aims at overcoming linguistic and cultural problems suffered by the Indonesians when learning the Arabic, especially their difficulty in understanding Arabic elements that are different from what in their language, and their difficulty  in using Arabic in certain situations properly.DOI: 10.15408/a.v2i2.2141

  20. Compuestos volátiles de brotes de limón persa y limón mexicano asociados a síntomas del HLB (Huanglongbing).

    OpenAIRE

    Mendoza Peña, Estrella

    2014-01-01

    El Huanglongbing (HLB) es una enfermedad de los cítricos que se caracteriza por causar áreas moteadas en hojas, generar frutos deformes y decolorados, semillas abortivas, causar pérdida paulatina de la producción y provocar que la muerte de los árboles infectados sea inevitable. El agente asociado a esta enfermedad es la bacteria Candidatus Liberibacter ssp., y en México es dispersada por el vector Diaphorina citri Kuwayama (Hemiptera: Liviidae). En el presente estudio, mediante el método de ...

  1. Zinc oxide nanoparticles: a 90-day repeated-dose dermal toxicity study in rats

    Directory of Open Access Journals (Sweden)

    Ryu HJ

    2014-12-01

    Full Text Available Hwa Jung Ryu,1,* Mu Yeb Seo,2,* Sung Kyu Jung,1 Eun Ho Maeng,2 Seung-Young Lee,2 Dong-Hyouk Jang,2 Taek-Jin Lee,2 Ki-Yeon Jo,2 Yu-Ri Kim,3 Kyu-Bong Cho,4 Meyoung-Kon Kim,3 Beom Jun Lee,5 Sang Wook Son1 1Department of Dermatology, Korea University College of Medicine, Seoul, 2Korea Testing and Research Institute, Gyunggido, 3Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul, 4Department of Clinical Laboratory Science, Shinheung College, Uijeongbu, 5College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea *These authors contributed equally to this work and both should be considered first authors Abstract: Zinc oxide (ZnO works as a long-lasting, broad-spectrum physical sunblock, and can prevent skin cancer, sunburn, and photoaging. Nanosized ZnO particles are used often in sunscreens due to consumer preference over larger sizes, which appear opaque when dermally applied. Although the US Food and Drug Administration approved the use of nanoparticles (NPs in sunscreens in 1999, there are ongoing safety concerns. The aim of this study was to evaluate the subchronic toxicity of ZnO NPs after dermal application according to the Organization for Economic Cooperation and Development Test Guidelines 411 using Good Laboratory Practice. Sprague Dawley rats were randomly divided into eight (one control, one vehicle control, three experimental, and three recovery groups. Different concentrations of ZnO NPs were dermally applied to the rats in the experimental groups for 90 days. Clinical observations as well as weight and food consumption were measured and recorded daily. Hematology and biochemistry parameters were determined. Gross pathologic and histopathologic examinations were performed on selected tissues from all animals. Analyses of tissue were undertaken to determine target organ tissue distribution. There was no increased mortality in the experimental group. Although there

  2. Die Einverleibung Holsteins im Jahre 1806 und die dänische Reaktion auf die Auflösung des Alten Reiches

    DEFF Research Database (Denmark)

    Bregnsbo, Michael

    2008-01-01

    Den danske statsledelses integrationspolitik over for hertugdømmet Holsten, der havde hørt under det Hellige Romerske Rige af Tysk Nation efter at dette rige var blevet opløst i 1806. Der argumenteres for, at integrationsbestræbelserne ikke udelukkende var dansk-nationale, men også udtryk for en ...

  3. Molekülinterne Dipolorientierung und dielektrische Absorption in verdünnter Lösung bei Mikro-und Submillimeterwellen. V.Acetyl-Verbindungen

    Science.gov (United States)

    Klages, Gerhard

    1989-09-01

    The dielectric loss of sixteen molecules, eleven with the acetyle group in aromatic bonds and five in aliphatic bonds, has been measured in very dilute solutions of cyclohexene at 20 °C. Five of these molecules are investigated in decalene solutions, too. The measurements have been made at wavenumbers in the range 0.08 to 140 cm -1 above 8 cm -1 making use of a pumped molecular laser. They are supplemented by the data of a Fourier transform spectrometer up to 300 cm -1. All microwave spectra of loss factor ɛ″ are analysed in terms of three absorption areas using the two variable Mori formalism. On the other hand, Lorentz curves are fitted to the FIR absorption spectra of α(v¯). The fast relaxation process of the aliphatic compounds is suggested to be due to intramolecular reorientation of the acetyle group itself, since in acetyle cyclohexene the remaining part of the molecule is rigid. To the contrary, the acetyle group is not able to reorientate swiftly, if bounded to aromatic rings. Presumably, it is fixed by mesomeric interaction with the dipole in the plain of the ring. However, 2-methyl acetophenone and 1-acetyle naphthone show some quick dipole reorientation, which coincides with a steric hindrance of the group in entering the plain of the ring.

  4. Syingir: Transformasi Puisi Arab ke dalam Puisi Jawa

    Directory of Open Access Journals (Sweden)

    Hindun

    2012-02-01

    Full Text Available Syingir is a advice poem that uses the daily Javanese Language and sung individually or together. Syingir developed in pesantren [lslamic boarding school] or in Islamic region, in Central Java and East Java. The creators are kyai or da'i. This poem created to spread the teachings of Islam including akidah, syariah, and muamalah by means of an easy to remember so it was easy to be understood. In terms of firm, syingir closer to the pattern of traditional Arabic poem, syi'ir known as the pattern of al-Khalil. Usage that pattern was caused by penyingir are kiais that had been familiar with ilmul arudl wal-'-qafiyah, the science of rhythm and rhyme in Arabic poetry. While in the term of content, syingir as a religious message is the transformation of the contents of the Arabic poetry which is at-ta'lim (education.

  5. LIM domain only-2 (LMO2 induces T-cell leukemia by two distinct pathways.

    Directory of Open Access Journals (Sweden)

    Stephen Smith

    Full Text Available The LMO2 oncogene is deregulated in the majority of human T-cell leukemia cases and in most gene therapy-induced T-cell leukemias. We made transgenic mice with enforced expression of Lmo2 in T-cells by the CD2 promoter/enhancer. These transgenic mice developed highly penetrant T-ALL by two distinct patterns of gene expression: one in which there was concordant activation of Lyl1, Hhex, and Mycn or alternatively, with Notch1 target gene activation. Most strikingly, this gene expression clustering was conserved in human Early T-cell Precursor ALL (ETP-ALL, where LMO2, HHEX, LYL1, and MYCN were most highly expressed. We discovered that HHEX is a direct transcriptional target of LMO2 consistent with its concordant gene expression. Furthermore, conditional inactivation of Hhex in CD2-Lmo2 transgenic mice markedly attenuated T-ALL development, demonstrating that Hhex is a crucial mediator of Lmo2's oncogenic function. The CD2-Lmo2 transgenic mice offer mechanistic insight into concordant oncogene expression and provide a model for the highly treatment-resistant ETP-ALL subtype.

  6. Bioaccumulation of heavy metals by bivalves from lim fjord (North Adriatic Sea)

    Energy Technology Data Exchange (ETDEWEB)

    Martincic, D.; Nurnberg, H.W.; Stoeppler, M.; Branica, M.

    1984-08-01

    The accumulation of trace metals (Zn, Cd, Pb, and Cu) by two bivalves (Mytilus galloprovincialis and Ostrea edulis) growing at the same station and therefore under the same physicochemical conditions was studied. The oysters were more effective bioaccumulators for zinc, cadmium, lead and copper than the mussels. Oysters accumulate about 10-fold higher quantities of zinc than mussels, 16-fold higher for copper, 3-fold higher cadmium, and 2-fold higher lead concentrations. The observed differences in the copper and zinc distribution within the investigated organs can be attributed to some recently recognized blood cells in the oyster which are rich in zinc and copper. The tissue copper and zinc distribution in this animal depends on where these cells are at the time of sampling. The mussel contains no such cells. 53 references, 7 figures, 1 table.

  7. Eesti maarahva välimääraja / Andres Kaera

    Index Scriptorium Estoniae

    Kaera, Andres

    2007-01-01

    Näitus "Eesti maarahvas baltisaksa kunstis" Eesti Rahva Muuseumis. A. von Uexkülli (autorsus küsimärgi all), Friedrich Sigismund Sterni, Ernst Hermann Schlichtingi, Theodor Gehlhaari ja August Georg Wilhelm Pezoldi töödest

  8. Fonemas segmentales en el criollo inglés de Limón

    OpenAIRE

    Portilla Chaves, Mario

    2015-01-01

    En este artículo se propone un nuevo análisis fonémico de los fonemas segmentales del criollo limonense, a diferencia de otros que se han puesto anteriormente. Ofrece una descripción de los fonemas segmentales (vocales y consonantes), la distribución principal de fonemas y de sus principales elementos incluidos. This article proposes a new phonemic analysis of the segmental phonemes of Limonese creole, opposed to other that have been put forth. A description of the segmental phonemes (vowels ...

  9. La inocuidad alimentaria en el mercado Mexicano de Limón Persa (Citrus latifolia tanaka)

    OpenAIRE

    Herbert Ruiz, Marlene

    2012-01-01

    La globalización comercial exige que los mercados mundiales de productos agroalimentarios observen prácticas de inocuidad en sus productos: libres de contaminantes químicos, biológicos y físicos. En ese contexto, México modificó el 26 de julio de 2007 la Ley Federal de Sanidad Vegetal (LFSV), la cual ahora tiene como objetivo la aplicación, verificación y certificación de los sistemas de reducción de riesgos de contaminación en la producción y empaque de vegetales. El obje...

  10. Ciliados epibiontes en Macrobrachium rosenbergii (De Man) cultivados en Limón, Costa Rica

    OpenAIRE

    Camacho, Lucía; Chinchilla, Misael

    2016-01-01

    Se identificaron los siguientes ciliados epibiontes en Macrobrachium rosenbergii: Epistylis, Opercularia, Vorticella, Zoothamnium, Ephelota, Acineta, Acinetides y Tolophrya. Acinetides se informa por primera vez como epibionte para camarones marinos y dulceacuícolas. The following epizoic ciliates were obtained from Macrobrachium rosenbergii: Epistylis, Opercularia, Vorticella, Zoothamnium, Ephelota, Acineta, Acinetides and Tokophrya. Acinetides is reported for the first time as an epizoic...

  11. Põhjamaade kunsti korraliku välimääraja leiab Kumust / Siram

    Index Scriptorium Estoniae

    Siram, pseud., 1968-

    2009-01-01

    Näitus "I Love Malmö" Kumu Kunstimuuseumis 17. jaanuarini 2010, kuraatorid Eha Komissarov ja Maria-Kristiina Soomre. Eksponeeritakse valikut Malmö kunstimuuseumi kogudest. Andy Warholilt on näitusel neli Mao Zedongi siiditrükis portreed. Joseph Beuys'i loomingust eksponeeritakse "Haisuskulptuuri". Nimetatud eksponeeritud töid ja nende autoreid

  12. Complete genome sequence of Cellulophaga lytica type strain (LIM-21T)

    Energy Technology Data Exchange (ETDEWEB)

    Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Abt, Birte [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Teshima, Hazuki [Los Alamos National Laboratory (LANL); Nolan, Matt [Joint Genome Institute, Walnut Creek, California; Lapidus, Alla L. [Joint Genome Institute, Walnut Creek, California; Lucas, Susan [Joint Genome Institute, Walnut Creek, California; Hammon, Nancy [Joint Genome Institute, Walnut Creek, California; Deshpande, Shweta [Joint Genome Institute, Walnut Creek, California; Cheng, Jan-Fang [Joint Genome Institute, Walnut Creek, California; Tapia, Roxanne [Los Alamos National Laboratory (LANL); Han, Cliff [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [Joint Genome Institute, Walnut Creek, California; Liolios, Konstantinos [Joint Genome Institute, Walnut Creek, California; Pagani, Ioanna [Joint Genome Institute, Walnut Creek, California; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [Joint Genome Institute, Walnut Creek, California; Palaniappan, Krishna [Joint Genome Institute, Walnut Creek, California; Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Detter, J. Chris [Joint Genome Institute, Walnut Creek, California; Brambilla, Evelyne-Marie [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Kannan, K. Palani [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Spring, Stefan [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Woyke, Tanja [Joint Genome Institute, Walnut Creek, California; Bristow, James [Joint Genome Institute, Walnut Creek, California; Eisen, Jonathan [Joint Genome Institute, Walnut Creek, California; Markowitz, Victor [Joint Genome Institute, Walnut Creek, California; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [Joint Genome Institute, Walnut Creek, California; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Ivanova, N [U.S. Department of Energy, Joint Genome Institute

    2011-01-01

    Cellulophaga lytica (Lewin 1969) Johansen et al. 1999 is the type species of the genus Cellulophaga, which belongs to the family Flavobacteriaceae within the phylum 'Bacteroidetes' and was isolated from marine beach mud in Limon, Costa Rica. The species is of biotechnological interest because its members produce a wide range of extracellular enzymes capable of degrading proteins and polysaccharides. After the genome sequence of Cellulophaga algicola this is the second completed genome sequence of a member of the genus Cellulophaga. The 3,765,936 bp long genome with its 3,303 protein-coding and 55 RNA genes consists of one circular chromosome and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  13. Ictioplancton en la zona portuaria de Limón, Costa Rica

    Directory of Open Access Journals (Sweden)

    Arturo Dominici-Arosemena

    2000-06-01

    Full Text Available Four sampling stations were set 200-500m off Limon Port, Costa Rica, at an average depth of 20 m. From October 1996 to May 1997 horizontal subsuperficial trawls were done for 12 minutes at a speed of 1.853 Km/h, with a 1 000 µm net were surveyed with. A total of 28 samples were processed and 104 physical-chemistry parameter measurements were taken. Average salinity was 30.1 + 3.7 and dissolved oxygen 6.9 + 0.6 mg l-1 reflecting good aeration throughout the sampling period. This suggests good mixture and a highly dynamic hydrography. Temperature showed no drastic variations (28.0 + 1.7 °C, possibly because of the constant mixing with shallow water. The highest larval counts were for November and early January and include families from reefs and estuaries (Balistidae, Lutjanidae and from coastal areas (Centropomidae, Gerreidae, Haemulidae, Carangidae, Engraulidae, Hemirramphidae, and representative Pleuronectiformes existing in a common area.

  14. 'PACLIMS': a component LIM system for high-throughput functional genomic analysis.

    Science.gov (United States)

    Donofrio, Nicole; Rajagopalon, Ravi; Brown, Douglas; Diener, Stephen; Windham, Donald; Nolin, Shelly; Floyd, Anna; Mitchell, Thomas; Galadima, Natalia; Tucker, Sara; Orbach, Marc J; Patel, Gayatri; Farman, Mark; Pampanwar, Vishal; Soderlund, Cari; Lee, Yong-Hwan; Dean, Ralph A

    2005-04-12

    Recent advances in sequencing techniques leading to cost reduction have resulted in the generation of a growing number of sequenced eukaryotic genomes. Computational tools greatly assist in defining open reading frames and assigning tentative annotations. However, gene functions cannot be asserted without biological support through, among other things, mutational analysis. In taking a genome-wide approach to functionally annotate an entire organism, in this application the approximately 11,000 predicted genes in the rice blast fungus (Magnaporthe grisea), an effective platform for tracking and storing both the biological materials created and the data produced across several participating institutions was required. The platform designed, named PACLIMS, was built to support our high throughput pipeline for generating 50,000 random insertion mutants of Magnaporthe grisea. To be a useful tool for materials and data tracking and storage, PACLIMS was designed to be simple to use, modifiable to accommodate refinement of research protocols, and cost-efficient. Data entry into PACLIMS was simplified through the use of barcodes and scanners, thus reducing the potential human error, time constraints, and labor. This platform was designed in concert with our experimental protocol so that it leads the researchers through each step of the process from mutant generation through phenotypic assays, thus ensuring that every mutant produced is handled in an identical manner and all necessary data is captured. Many sequenced eukaryotes have reached the point where computational analyses are no longer sufficient and require biological support for their predicted genes. Consequently, there is an increasing need for platforms that support high throughput genome-wide mutational analyses. While PACLIMS was designed specifically for this project, the source and ideas present in its implementation can be used as a model for other high throughput mutational endeavors.

  15. 'PACLIMS': A component LIM system for high-throughput functional genomic analysis

    Directory of Open Access Journals (Sweden)

    Farman Mark

    2005-04-01

    Full Text Available Abstract Background Recent advances in sequencing techniques leading to cost reduction have resulted in the generation of a growing number of sequenced eukaryotic genomes. Computational tools greatly assist in defining open reading frames and assigning tentative annotations. However, gene functions cannot be asserted without biological support through, among other things, mutational analysis. In taking a genome-wide approach to functionally annotate an entire organism, in this application the ~11,000 predicted genes in the rice blast fungus (Magnaporthe grisea, an effective platform for tracking and storing both the biological materials created and the data produced across several participating institutions was required. Results The platform designed, named PACLIMS, was built to support our high throughput pipeline for generating 50,000 random insertion mutants of Magnaporthe grisea. To be a useful tool for materials and data tracking and storage, PACLIMS was designed to be simple to use, modifiable to accommodate refinement of research protocols, and cost-efficient. Data entry into PACLIMS was simplified through the use of barcodes and scanners, thus reducing the potential human error, time constraints, and labor. This platform was designed in concert with our experimental protocol so that it leads the researchers through each step of the process from mutant generation through phenotypic assays, thus ensuring that every mutant produced is handled in an identical manner and all necessary data is captured. Conclusion Many sequenced eukaryotes have reached the point where computational analyses are no longer sufficient and require biological support for their predicted genes. Consequently, there is an increasing need for platforms that support high throughput genome-wide mutational analyses. While PACLIMS was designed specifically for this project, the source and ideas present in its implementation can be used as a model for other high throughput mutational endeavors.

  16. 'PACLIMS': A component LIM system for high-throughput functional genomic analysis

    OpenAIRE

    Farman Mark; Patel Gayatri; Orbach Marc J; Tucker Sara; Galadima Natalia; Mitchell Thomas; Floyd Anna; Nolin Shelly; Windham Donald; Diener Stephen; Brown Douglas; Rajagopalon Ravi; Donofrio Nicole; Pampanwar Vishal; Soderlund Cari

    2005-01-01

    Abstract Background Recent advances in sequencing techniques leading to cost reduction have resulted in the generation of a growing number of sequenced eukaryotic genomes. Computational tools greatly assist in defining open reading frames and assigning tentative annotations. However, gene functions cannot be asserted without biological support through, among other things, mutational analysis. In taking a genome-wide approach to functionally annotate an entire organism, in this application the...

  17. End-to-End Models for Effects of System Noise on LIMS Analysis of Igneous Rocks

    Energy Technology Data Exchange (ETDEWEB)

    Clegg, Samuel M [Los Alamos National Laboratory; Bender, Steven [Los Alamos National Laboratory; Wiens, R. C. [Los Alamos National Laboratory; Carmosino, Marco L [MT. HOLYOKE COLLEGE; Speicher, Elly A [MT. HOLYOKE COLLEGE; Dyar, M. D. [MT. HOLYOKE COLLEGE

    2010-12-23

    The ChemCam instrument on the Mars Science Laboratory will be the first extraterrestial deployment of laser-induced breakdown spectroscopy (UBS) for remote geochemical analysis. LIBS instruments are also being proposed for future NASA missions. In quantitative LIBS applications using multivariate analysis techniques, it is essential to understand the effects of key instrument parameters and their variability on the elemental predictions. Baseline experiments were run on a laboratory instrument in conditions reproducing ChemCam performance on Mars. These experiments employed Nd:YAG laser producing 17 mJ/pulse on target and an with a 200 {micro}m FWHM spot size on the surface of a sample. The emission is collected by a telescope, imaged on a fiber optic and then interfaced to a demultiplexer capable of >40% transmission into each spectrometer. We report here on an integrated end-to-end system performance model that simulates the effects of output signal degradation that might result from the input signal chain and the impact on multivariate model predictions. There are two approaches to modifying signal to noise (SNR): degrade the signal and/or increase the noise. Ishibashi used a much smaller data set to show that the addition of noise had significant impact while degradation of spectral resolution had much less impact on accuracy and precision. Here, we specifically focus on aspects of remote LIBS instrument performance as they relate to various types of signal degradation. To assess the sensitivity of LIBS analysis to signal-to-noise ratio (SNR) and spectral resolution, the signal in each spectrum from a suite of 50 laboratory spectra of igneous rocks was variably degraded by increasing the peak widths (simulating misalignment) and decreasing the spectral amplitude (simulating decreases in SNR).

  18. Labour Costs for Inventory Control Less Expensive than Repurchasing. A Review of: Sung, J. S., Whisler, J. A., & Sung, N. (2009. A cost-benefit analysis of a collections inventory project: A statistical analysis of inventory data from a medium-sized academic library. Journal of Academic Librarianship, 35(4, 314-323.

    Directory of Open Access Journals (Sweden)

    Laura Newton Miller

    2010-09-01

    Full Text Available Objective – To describe an inventory system that was created within the library and to show the cost-effectiveness of using the inventory system compared to the price of reacquiring mis-shelved books.Design – Bibliometric study and cost-benefit analysis.Setting – Medium-sized academic library in a rural community of the United States.Subjects – Approximately 300,000 books from LC classifications D through H, N, P and Q, representing two thirds of the library’s entire monograph collection.Methods – The library created its own electronic inventory and shelf-reading program, using a laptop computer equipped with a hand-held scanner, to scan barcodes in the stacks. Library staff used the Microsoft Access database to update two files containing a shelf-list and an active-status list while the books were scanned. The program alerted the worker if books found had an active status (i.e., Missing, Renewed, Overdue, Charged, were not in the correct order, or were not in the system. Each transaction created a log which contained a time stamp (to the second, the call number and the barcode number. It also took note of scanning errors, books that were out of order, and books that were not on a shelf-list. After a complete section was examined, a list was produced to reveal the distance of mis-shelved books from their correct location and the amount of time between each scan. The researchers used statistical analysis (using SPSS 15.0 to measure scan speed for each scan, mis-shelving rate and error distance of each mis-shelved book. In order to analyze the cost of labour to replace a book versus the cost of inventorying, the researchers estimated the salary costs of staff members involved in selection, acquisition and cataloguing. The library spent $440,000 USD in labour costs to purchase 15,000 monographs in one fiscal year (approximately $30.00 USD in labour costs per book. They multiplied this by 5300 books that were found to be “badly” mis-shelved (found beyond 25 books away from the proper position. Labour fees were used to determine costs of inventorying by calculating average scanning speed and cost per hour to pay someone to scan the entire half-million monograph collection.Main Results – It took approximately 707 hours to scan 305,000 monographs. The average (mode calculation for scans was 5 seconds for 80% of the barcodes, with an average (mean of 8.35 seconds between scans. The longest average (mean time for scanning barcodes was in the N section, followed by G, H, P, Q, D, E and F. A total of 291 books were found on the shelves with an “active” status (i.e., Charged (4, Overdue (7, Renewed (4, In Transit (24, and Missing (228. Twenty-four books with the status “Miscellaneous” (i.e., At Bindery, Call Slip, Cataloguing Review, Damaged, and Mending were also found on the shelves. Of the 15 active books in the categories “Charged”, “Overdue” and “Renewed”, ten were found in the proper position on the shelf. Of the 228 “Missing” books, 30% were scanned in the correct location, 10% were found 26 to 100 books away, and half were located over 100 books from their proper location. In addition to the books already marked as “Missing” in the catalogue, there were 516 books (.17% of the entire scanned section still not found on the shelf after three searches over a period of 6 months. Of the 291 active status books found on the shelves, 52% were reused as of July 2008. (The inventory was completed at the end of 2006. Over 36% of books mis-shelved further than 25 books from their correct location were reused. However, among all books scanned, only 17% were reused during the same time period. The researchers noted that inconsistencies between the call number as shown on the book label and how it appeared in the catalogue occurred 565 times. Of these discrepancies, 40% of the labels resulted in books being misplaced ten or fewer books away, 10% misplaced between 10 and 100 books away, and 35% misplaced more than 100 books away from the correct position. In general, 82% of mis-shelved books were found within 1 to 25 books away from their correct location. By calculating that 5300 books were mis-shelved beyond 25 books away from their proper position, labour costs were estimated to be at least $159,000 USD (5300 x $30.00 USD per book in labour costs. Costs for interlibrary loan were calculated at approximately $30.00 USD per transaction, and patron’s time wasted trying to locate misplaced books was estimated at 30 minutes per book. This was much more than the labour costs associated with scanning books, which at an average speed of 8 seconds per book and $10.00 US per hour for scanning worked out to be 2.2 cents per book, or $11,000 USD to scan the entire half-million monograph collection.Conclusion – The results appear to reveal that the labour costs for inventory control are less expensive than repurchasing or borrowing the same number of books.

  19. Labour Costs for Inventory Control Less Expensive than Repurchasing. A Review of: Sung, J. S., Whisler, J. A., & Sung, N. (2009). A cost-benefit analysis of a collections inventory project: A statistical analysis of inventory data from a medium-sized academic library. Journal of Academic Librarianship, 35(4), 314-323.

    OpenAIRE

    Laura Newton Miller

    2010-01-01

    Objective – To describe an inventory system that was created within the library and to show the cost-effectiveness of using the inventory system compared to the price of reacquiring mis-shelved books.Design – Bibliometric study and cost-benefit analysis.Setting – Medium-sized academic library in a rural community of the United States.Subjects – Approximately 300,000 books from LC classifications D through H, N, P and Q, representing two thirds of the library’s entire monograph collection.Meth...

  20. To cope with new problems of energy situation (part 2); Understanding the international trend of rapidly developing global environmental problems. Energy josei no atarashii kadai ni ikani taio suruka (Kenkyu hokoku 2); Kyu tenkai wo miseta chikyu kankyo mondai no kokusaiteki nagare wo toraeru

    Energy Technology Data Exchange (ETDEWEB)

    Tomidate, Takao (The Inst. of Energy Economics, Tokyo, (Japan))

    1990-02-01

    It is now world-widely recognized that, though there are many unclear points regarding the effects of environmental problems on society and economics, delayed actions for protecting environment will bring unrecoverable disruption which causes disaster to later generations. Especially, global warming is the most difficult problem to elucidate the phenomenon for finding countermeasure, and influences of it and impacts brought by practising the countermeasures are serious. Among the causes, it is said that most important one is emission of carbon dioxide by burning fossil fuel, and most of the countermeasures are focused to that. For the reason, it is understood that effect of the problem on future energy is unconceivably serious. In this report, political aspect of global warming are introduced as ther most important global environmental problems and guidances to the future countermeasures for Japan are explained. 2 figs., 2 tabs.

  1. What’s in the pipeline? Drugs in development for autism spectrum disorder

    Directory of Open Access Journals (Sweden)

    Sung M

    2014-02-01

    Full Text Available Min Sung,1 Chee Hon Chin,1 Choon Guan Lim,1 Hwee Sen Alvin Liew,1 Chau Sian Lim,2 Espérance Kashala,1 Shih-Jen Weng1 1Department of Child and Adolescent Psychiatry, Institute of Mental Health, Singapore; 2Department of Psychological Medicine, Khoo Teck Puat Hospital, Singapore Abstract: Autism Spectrum Disorder (ASD is a complex neurodevelopmental disorder with both core symptoms and associated symptoms (eg, irritability, aggression, and comorbidities that affect both the individual and the family/systems around them. There have been recent advances in the understanding of the underlying pathophysiology of ASD pertaining to genetics, epigenetics, neurological, hormonal, and environmental factors that contribute to the difficulties found in individuals with ASD. With this improved understanding, there has been a shift in the application of psychopharmacology in ASD and its related disorders. A literature review was conducted to examine research published in the last 5 years between different classes of psychotropic medications and ASD. The broad scope of the existing literature for the use of conventional medications is summarized and novel medications are discussed. Keywords: pharmacology, treatment, autism, Asperger’s syndrome, medication

  2. 实验室信息管理系统(LIMS)系统在玉米深加工企业中的应用%The application of laboratory information management system (LIMS) in corn deep processing enterprise

    Institute of Scientific and Technical Information of China (English)

    杨维旭; 董秀玲; 王冰; 赵铁巍

    2003-01-01

    @@ 1 概述 1.1 LIMS的基础概念 LIMS全称是"Laboratory information management system",即实验室信息管理系统.是基于GMP(优良生产管理规范)及GLP(优良实验室管理规范)的主导思想,以实验室为核心的整体环境管理软件,其集样品管理、数据管理(输入、处理、发布)、网络管理、报表管理等诸多模块为一体,组成一套完整的实验室综合管理和产品质量监控体系,对影响实验室质量的要素进行全面管理和控制,既能满足外部的日常管理要求,又能保证实验室分析数据的严格管理和控制,从而提高实验室管理的整体水平.

  3. LABWARE LIMS:实验室管理的优化者——访LABWARE亚太区总裁Dr.David McClelland%LABWARE LIMS Optimizes the Laboratory Management

    Institute of Scientific and Technical Information of China (English)

    马永娇

    2009-01-01

    @@ David McClelland:毕业于英国利兹大学遗传学专业,曾在英国VG公司从事LIMS开发工作10余年,在LIMS方面积累了丰富的经验.2000年加入LabWare公司,现任LabWare亚太区总裁,兼北京莱博韦尔科技有限公司法人代表.

  4. El arlequín de limón: una joya en extinción

    Directory of Open Access Journals (Sweden)

    Paula Peña

    2013-09-01

    Full Text Available Estos malignos y pestilentes animales…son detestables por su cuerpo frío, su palidez, esqueleto cartilaginoso, piel desagradable, aspecto feroz, ojos calculadores, olor irritante, voz chillona, moradas miserables y terrible veneno”. Así describió el naturalista sueco Carl Von Linneo en 1758 a los anfibios (Linneo, citado por Wells, 2007: 1. En la actualidad la reputación de sapos y ranas ha mejorado considerablemente, pero todavía existen aquellos que tienen una idea equivocada de estos animales. Lo que estas personas no saben es que las ranas y sapos son elementos de extrema importancia en los ecosistemas naturales, la medicina y la industria turística.

  5. Precise lim sup behavior of probabilities of large deviations for sums of i.i.d. random variables

    Directory of Open Access Journals (Sweden)

    Andrew Rosalsky

    2004-12-01

    Full Text Available Let {X,Xn;n≥1} be a sequence of real-valued i.i.d. random variables and let Sn=∑i=1nXi, n≥1. In this paper, we study the probabilities of large deviations of the form P(Sn>tn1/p, P(Sntn1/p, where t>0 and 0x1/p/ϕ(x=1, then for every t>0, limsupn→∞P(|Sn|>tn1/p/(nϕ(n=tpα.

  6. Argonaute Utilization for miRNA Silencing Is Determined by Phosphorylation-Dependent Recruitment of LIM-Domain-Containing Proteins

    Directory of Open Access Journals (Sweden)

    Katherine S. Bridge

    2017-07-01

    Full Text Available As core components of the microRNA-induced silencing complex (miRISC, Argonaute (AGO proteins interact with TNRC6 proteins, recruiting other effectors of translational repression/mRNA destabilization. Here, we show that LIMD1 coordinates the assembly of an AGO-TNRC6 containing miRISC complex by binding both proteins simultaneously at distinct interfaces. Phosphorylation of AGO2 at Ser 387 by Akt3 induces LIMD1 binding, which in turn enables AGO2 to interact with TNRC6A and downstream effector DDX6. Conservation of this serine in AGO1 and 4 indicates this mechanism may be a fundamental requirement for AGO function and miRISC assembly. Upon CRISPR-Cas9-mediated knockout of LIMD1, AGO2 miRNA-silencing function is lost and miRNA silencing becomes dependent on a complex formed by AGO3 and the LIMD1 family member WTIP. The switch to AGO3 utilization occurs due to the presence of a glutamic acid residue (E390 on the interaction interface, which allows AGO3 to bind to LIMD1, AJUBA, and WTIP irrespective of Akt signaling.

  7. PARPs database: A LIMS systems for protein-protein interaction data mining or laboratory information management system

    Science.gov (United States)

    Droit, Arnaud; Hunter, Joanna M; Rouleau, Michèle; Ethier, Chantal; Picard-Cloutier, Aude; Bourgais, David; Poirier, Guy G

    2007-01-01

    Background In the "post-genome" era, mass spectrometry (MS) has become an important method for the analysis of proteins and the rapid advancement of this technique, in combination with other proteomics methods, results in an increasing amount of proteome data. This data must be archived and analysed using specialized bioinformatics tools. Description We herein describe "PARPs database," a data analysis and management pipeline for liquid chromatography tandem mass spectrometry (LC-MS/MS) proteomics. PARPs database is a web-based tool whose features include experiment annotation, protein database searching, protein sequence management, as well as data-mining of the peptides and proteins identified. Conclusion Using this pipeline, we have successfully identified several interactions of biological significance between PARP-1 and other proteins, namely RFC-1, 2, 3, 4 and 5. PMID:18093328

  8. Miniature LIMS system designed for sensitive in situ measurements of organic deposits in materials on solar system objects

    Science.gov (United States)

    Riedo, Andreas; Moreno-García, Pavel; Grimaudo, Valentine; Brigitte Neuland, Maike; Tulej, Marek; Broekmann, Peter; Wurz, Peter

    2014-05-01

    In situ investigation of chemical composition is of considerable interest for application in planetary missions. The possibility of bio-relevant organic chemistry on the surfaces of comets and primitive asteroids is one of the most intriguing problems of the current astrobiology. Rock-water interface existed in early small solar body history, in addition to extensive mineralogical modifications of the surface could have initiated also a rich organic and bio-organic chemistry [1]. Current space instrumentation designed for molecular detection use thermal evaporation source combined with gas chromatography (GC) and mass spectrometry [2]. Hence, it is capable of collecting data of the volatile fraction of the investigated sample. Laser desorption mass spectrometry (LD-MS) is another method for molecule detection capable of vaporisation and ionisation of molecules present on a solid substrate surface, e.g. a rock. A miniature laser ablation ionisation reflectron-type time-of-flight mass analyser (LMS) developed by our group for in situ measurements of the elemental, isotopic composition can be used also for sensitive analyses of chemical compounds present on solid surfaces with high spatial resolution [3-6]. Comparing to laser ablation laser desorption analyses are conducted at laser irradiance reduced by approximately 103-104 times. In laser ablation mode, the LMS system offers high dynamic range of at least eight orders of magnitude and allows for the detection in the ppb range for metallic- and non-metallic elements. Instrument mass resolution m/Δm is about 800 at the 56Fe mass peak when the instrument is used in ablation mode. The mass resolution is increased to m/Δm >1000 when desorption studies are conducted (at ~600 m/q). We will demonstrate the instrument performance conducting molecular desorption studies of different species, e.g. organic, biotic and abiotic. Laser ablation/ionisation studies are conducted in parallel to complement the laser desorption analyses. References 1)S. Pizzarello, Y. Huang, M.R. Alexandre, 'Molecular asymmetry in extraterrestrial chemistry: insights from a pristine meteorite', PNAS, 2008, 105, pp. 3700 - 3704. 2)L. A. Leshin et al., Volatile, isotope, and organic analysis of Martian fines with the Mars Curiosity rover, Science, 2013, 341, 1238937, pp. 1 - 9. 3)A. Riedo, M. Neuland, S. Meyer, M. Tulej, and P. Wurz, 'Coupling of LMS with a fs-laser ablation ion source: elemental and isotope composition measurements', J. Anal. At. Spectrom., 2013, 28, pp. 1256 - 1269. 4)A. Riedo, S. Meyer, B. Heredia, M.B. Neuland, A. Bieler, M. Tulej, I. Leya, M. Iakovleva, K. Mezger, and P. Wurz, 'Highly accurate isotope composition measurements by a miniature laser ablation mass spectrometer designed for in situ investigations on planetary surfaces', Planet. Space Sci., 2013, 87, pp. 1 - 13. 5)A. Riedo, P. Wahlström, J.A. Scheer, P. Wurz, and M. Tulej, 'Effect of long duration UV irradiation on diamondlike carbon surfaces in the presence of a hydrocarbon gaseous atmosphere', J. Appl. Phys., 2010, 108, 114915, pp.1 - 8. 6)M. Tulej, M. Iakovleva, I. Leya, and P. Wurz, 'A miniature mass analyser for in-situ elemental analysis of planetary material - performance studies', Anal. Bioanal. Chem., 2011, 399, pp. 2185 - 2200.

  9. Caracterización de un motor lineal de inducción : linear induction motor (L.I.M.)

    OpenAIRE

    Pérez Rodríguez, Carlos

    2011-01-01

    Se parte de un prototipo ya construido, del que se desconocen sus características eléctricas y magnéticas, y por lo tanto se desconoce su funcionamiento, por lo que el trabajo ha consistido en la definición del funcionamiento nominal del motor lineal de inducción, mediante el estudio de la inducción magnética presente en el entrehierro y de la curva característica empuje – velocidad. Se disponía del primario de la máquina (armadura), pero no se disponía del secundario (jaula de ardilla). ____...

  10. [DNA damage in female workers exposed to pesticides in banana plantations at Limón, Costa Rica].

    Science.gov (United States)

    Ramírez, Vanessa; Cuenca, Patricia

    2002-06-01

    Pesticide use in Costa Rica is very high and all year round. A high percentage of what is sprayed remains in the environment and in the living organisms around. This situation brings contamination and health problems to people in contact with them. The onset of adverse effects may be in the short or the long term, and symptoms vary widely, from headaches to cancer. Much research in this area has been devoted to acute or chronic effects, and not until recently to the genotoxic effect of pesticides. This study evaluated the genotoxic effect of pesticides used in banana packing activities, using the comet assay (single cell electrophoresis) as the biological marker in lymphocytes. This was a case-control double blind study of 30 exposed women from 15 banana farms and 28 women not occupationally exposed to pesticides from the same geographic area. Results show damage to single stranded DNA after working from 5 to 15 years (R2 = 0.12). In Costa Rica we do not have an historical record of the kind of pesticides used in banana farms, the period of time and for how long were they used. This prevented further analysis concerning dose, frequency of exposure and use of new or old kind of pesticides in the farms in relation to DNA damage. The comet assay is of value in the genetic monitoring of pesticide exposed populations.

  11. Daño del ADN en trabajadoras bananeras expuestas a plaguicidas en Limón, Costa Rica

    Directory of Open Access Journals (Sweden)

    Vanessa Ramírez

    2002-06-01

    Full Text Available El uso de plaguicidas en los países en desarrollo es extenso en cantidad y en tiempo. Un gran porcentaje de lo que se aplica queda en el entorno y en los organismos que ahí habitan, lo que acarrea problemas de contaminación ambiental y deterioro de la salud de las personas que están en contacto con ellos. Los efectos se pueden manifestar a corto o a largo plazo y los síntomas varían desde un dolor de cabeza hasta el desarrollo de cáncer. La mayoría de los estudios en esta área se han orientado hacia los efectos agudos o crónicos y recientemente se inicia el estudio del efecto genotóxico de los plaguicidas. En e1 presente trabajo se evaluó el efecto genotóxico de los plaguicidas utilizados en las empacadoras de banano, usando como marcador biológico la electroforesis de células únicas (conocido como ensayo cometa en linfocitos aislados de sangre periférica. La población en estudio correspondió a 30 trabajadoras expuestas a plaguicidas, procedentes de 15 fincas bananeras y 28 mujeres sin antecedentes de exposición ocupacional a plaguicidas, de la misma área geográfica. Las trabajadoras que tenían entre cinco y quince años de trabajo presentaron mayor daño en la hebra sencilla del ADN (R² =0.12. Se desconoce si este efecto se debe a los plaguicidas que se utilizan actualmente o a plaguicidas empleados en el pasado. Tampoco fue posible correlacionar las dosis y las frecuencias de exposición a los plaguicidas con el daño al ADN, debido a que no existe un registro histórico sobre los productos empleados en el cultivo de banano, no se sabe por cuánto tiempo y las épocas en que fueron utilizados. Se recomienda el ensayo cometa para monitoreo genético en poblaciones expuestas a plaguicidasPesticide use in Costa Rica is very high and all year round. A high percentage of what is sprayed remains in the environment and in the living organisms around. This situation brings contamination and health problems to people in contact with them. The onset of adverse effects may be in the short or the long term, and symptoms vary widely, from headaches to cancer. Much research in this area has been devoted to acute or chronic effects, and not until recently to the genotoxic effect of pesticides. This study evaluated the genotoxic effect of pesticides used in banana packing activities, using the comet assay (single cell electrophoresis as the biological marker in lymphocytes. This was a case-control double blind study of 30 exposed women from 15 banana farms and 28 women not occupationally exposed to pesticides from the same geographic area. Results show damage to single stranded DNA after working from 5 to 15 years (R 2 =0.12. In Costa Rica we do not have an historical record of the kind of pesticides used in banana farms, the period of time and for how long were they used. This prevented further analysis concerning dose, frequency of exposure and use of new or old kind of pesticides in the farms in relation to DNA damage. The comet assay is of value in the genetic monitoring of pesticide exposed populations

  12. PARPs database: A LIMS systems for protein-protein interaction data mining or laboratory information management system

    Directory of Open Access Journals (Sweden)

    Picard-Cloutier Aude

    2007-12-01

    Full Text Available Abstract Background In the "post-genome" era, mass spectrometry (MS has become an important method for the analysis of proteins and the rapid advancement of this technique, in combination with other proteomics methods, results in an increasing amount of proteome data. This data must be archived and analysed using specialized bioinformatics tools. Description We herein describe "PARPs database," a data analysis and management pipeline for liquid chromatography tandem mass spectrometry (LC-MS/MS proteomics. PARPs database is a web-based tool whose features include experiment annotation, protein database searching, protein sequence management, as well as data-mining of the peptides and proteins identified. Conclusion Using this pipeline, we have successfully identified several interactions of biological significance between PARP-1 and other proteins, namely RFC-1, 2, 3, 4 and 5.

  13. Spektralphotometrische Bestimmung des pflanzenverfügbaren Nitrats in der Bodenlösung : Entwicklung einer in-situ Messmethode zur Optimierung der Fertigation im intensiven Gemüsebau

    OpenAIRE

    Mayer, Stephan

    2015-01-01

    Bei vielen Sonderkulturen, hauptsächlich aber im intensiven Gemüsebau, kann sich bereits ein geringer Mangel an Stickstoff stark qualitäts- und ertragsmindernd auswirken. Daher wird besonders dort häufig im Überschuss gedüngt. Dies bedeutet, im Vergleich zu dem tatsächlichen N-Bedarf einer Kultur, oft einen erhöhten Aufwand an Dünger, was zu starken N-Verlusten durch Auswaschung führen kann. Stark nitratbelastete Grund- und Oberflächengewässer sind vielerorts die Folge. Eine in-situ Metho...

  14. A multicenter study of mid urethral sung procedures in treatment of female stress urinary incontinence%中段尿道吊带术治疗女性压力性尿失禁的多中心研究

    Institute of Scientific and Technical Information of China (English)

    杜广辉; 陈忠; 胡卫锋; 章传华; 张景宇; 祝子清; 叶章群

    2008-01-01

    目的 探讨中段尿道吊带术治疗女性压力性尿失禁的临床疗效、手术指征和并发症的处理.方法 单纯压力性尿失禁的患者304例和混合性尿失禁的患者8例,均为女性,纳入本研究.其中接受TVT吊带术134例,TVTO吊带术167例,Monarc吊带术11例.围手术期评估包括:手术时间、出血量、术中术后并发症.手术效果分为治愈、显效、无效.在患者出院时、术后3个月、术后每年评价疗效和并发症.结果 TVT组手术时间(18.5±9.6)min和出血量(32.2±12.6)ml多于TVTO组(11.5±3.1)min,(12.8±8.5)ml和Monarc组(11.1±2.6)min,(12.3±3.5)ml(P0.05).单纯压力性尿失禁患者治愈率(95.7% )显著高于混合性尿失禁患者(37.5% )(P<0.01).3种术式的总并发症发生率差异无统计学意义,但膀胱损伤仅发生在TVT组,闭孔神经损伤和阴道损伤仅发生在TVTO组.术后排尿困难和尿潴留是中段尿道吊带术的最常见并发症.结论 经耻骨上途径(TVT)和经闭孔途径(TVTO、Monarc)中段尿道吊带术治疗压力性尿失禁均具有手术简单、微创、中长期疗效好、并发症少等优点.%Objective To investigate the clinical efficacy,surgical indications and postoperative complications of mid urethral sling procedures in treatment of female stress urinary incontinence.Methods A muhicenter clinical trial was conducted from April 2002 to April 2008 in five hospitals,304 cases of genuine stress urinary incontinence and 8 cases of mixed incontinence were included.TVT procedures were carried out in 134 patients.TVTO procedures in 167 patients,Monare procedures in 11 patients.Perioperative evaluations included: operating time, bleeding volume, and perioperative complications.Operative efficacy was classified into three categories:cure,improved and failure and evaluated before discharge,3 months after surgery and then every year. Results TVT group had longer operating time [(18.5±9.6)min]and more bleeding volume[(32.2±12.6)ml]than those in TVTO group[(11.5 ±3.1)min,(12.8±8.5)m]and in Monarc group[(11.1±2.6)min,(12.3±3.5)ml]with P<0.05.Monarc and TVTO procedures had higher cure rates and impmve rates comparing with TVT,but the differences were of no significance.The cure rate(95.7% )in patients with genuine stress incontinence were significantly higher than that in patients with mixed incontinence(37.5% ).No significant differences oftotal intra- and postoperative complications were noted for all of the three procedures.However,bhdder injury tended to occuned in TVT group and obturator nerve injury and vaginal injury tended to occurre in TVTO group.Transient voiding dysfunction and urinary retention were the most common complications.Conclusions Mid urethral sling procedures have excellent clinical outcomes in the treatment of female stress urinary incontinence.

  15. Bổ sung giun quế (Perionyx excavatus) cho gà thịt (Hồ x Lương Phượng) từ 4 - 10 tuần tuổi

    OpenAIRE

    Vu, Dinh Ton; Han Quang, Hanh; Nguyen, Dinh Linh; Dang, Vu Binh

    2009-01-01

    The present study was aimed to assess the effect of different levels of red worm (Perionyx excavatus) supplemented to the diet on growth performance and meat quality of broilers. An experiment was conducted on broilers (Ho x Luong Phuong) of 4 - 10 weeks old according to a completely randomized design with one unsupplemented group (control) and three supplemented groups corresponding to 3 worm levels in the diet, viz. 1%, 1.5%, and 2% on a dry matter basis. Results showed that th...

  16. Report on technological development of fuel cell power generation 1998. Research and development on polymer electrolyte fuel cell (technological development of generating system, development of atmospheric operation home power system of several kW class); 1998 nendo seika hokokusho. Nenryo denchi hatsuden gijutsu kaihatsu, kotai kobunshigata nenryo denchi no kenkyu kaihatsu, hatsuden system gijutsu kaihatsu, joatsu sadogata su kW kyu kateiyo dengen system no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    This paper explains the results of the research and development of polymer electrolyte fuel cells in fiscal 1998. For the purpose of developing a power source suitable for household use, a cell unit was developed in which performance was improved by reducing the thickness of a catalyst layer and increasing its ion-exchange capacity in the fuel cell body. There were prospects that low cost carbon materials and resin materials were usable in a carbon/resin composite plate. In the fuel supply technology, a serial two step type remover was developed concerning CO in a fuel using a selective oxidation process, which confirmed that the exit concentration was reducible to 10ppm or below even under a low S/C condition. With a reform ratio of 95% achieved with a 1kW class natural gas reformer, a thermal efficiency of 85% was attained by efficiently using combustion exhaust gas/reform gas sensible heat. In order to achieve a power generating efficiency of 35%, it is essential to improve reformer heat efficiency and cell performance, and also to produce steam for reforming by effectively utilizing reform gas/burner exhaust gas. The 1kW class module was verified in its stable operation and performance. (NEDO)

  17. Types of Vasculitis

    Science.gov (United States)

    ... muscle weakness and paralysis (an inability to move). Kawasaki Disease Kawasaki (KAH-wah-SAH-ke) disease is a ... in the body, including arteries, veins, and capillaries. Kawasaki disease also is known as mucocutaneous (myu-ko-kyu- ...

  18. Experiment list: SRX186749 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 2 || softwareversion=ScriptureVPaperR3 || cell sex=F || antibody=H3K9me3 || antibody antibodydescription=rab...ut/std || softwareversion=ScriptureVPaperR3 http://dbarchive.biosciencedbc.jp/kyu

  19. Experiment list: SRX810427 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available iments were harvested when 80-90% confluent. || amplification cycles=10 || map quality cut-off=...20 || mononucleosome cut-off=190 || reference genome=dm3 http://dbarchive.biosciencedbc.jp/kyu

  20. Experiment list: SRX810430 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available riments were harvested when 80-90% confluent. || amplification cycles=9 || map quality cut-off=...20 || mononucleosome cut-off=190 || reference genome=dm3 http://dbarchive.biosciencedbc.jp/kyu

  1. Toxicity of colloidal silica nanoparticles administered orally for 90 days in rats

    Directory of Open Access Journals (Sweden)

    Kim YR

    2014-12-01

    Full Text Available Yu-Ri Kim,1,* Seung-Young Lee,3,* Eun Jeong Lee,1 Sung Ha Park,4 Nak-won Seong,3 Heung-Sik Seo,3 Sung-Sup Shin,3 Seon-Ju Kim,3 Eun-Ho Meang,3 Myeong-Kyu Park,3 Min-Seok Kim,3 Cheol-Su Kim,5 Soo-Ki Kim,5 Sang Wook Son,2 Young Rok Seo,6 Boo Hyon Kang,7 Beom Seok Han,8 Seong Soo A An,9 Beom-Jun Lee,10 Meyoung-Kon Kim1 1Department of Biochemistry and Molecular Biology, 2Department of Dermatology, Korea University Medical School and College, 3General Toxicology Team, Korea Testing and Research Institute, Seoul, Republic of Korea; 4Department of Biochemistry, University of Bath, Bath, UK; 5Department of Microbiology, Wonju College of Medicine, Yonsei University, Gangwon, 6Department of Life Science, Institute of Environmental Medicine for Green Chemistry, Dongguk University, Seoul, 7Nonclinical Research Institute, Chemon Inc, Gyeonggi, 8Toxicological Research Center, Hoseo University, Chungnam, 9Department of Bionanotechnology, Gachon University, Gyeonggi, 10College of Veterinary Medicine, Chungbuk National University, Chungbuk, Republic of Korea *These authors contributed equally to this work Abstract: This study was undertaken to investigate the potential toxicity and establish the no observed adverse effect level (NOAEL and target organ(s of negatively charged colloidal silica particles of different sizes, ie, SiO2EN,20(- (20 nm or SiO2EN,100(- (100 nm, administered by gavage in Sprague-Dawley rats. After verification of the physicochemical properties of the SiO2 particles to be tested, a preliminary dose range-finding study and 90-day repeated dose study were conducted according to the Organisation for Economic Cooperation and Development test guideline. Based on the results of the 14-day dose range-finding study, a high dose was determined to be 2,000 mg/kg, and middle and low doses were set at 1,000 and 500 mg/kg, respectively. In the 90-day toxicity study, there were no animal deaths in relation to administration of SiO2 particles of

  2. Comparative toxicity and biodistribution of copper nanoparticles and cupric ions in rats

    Directory of Open Access Journals (Sweden)

    Lee IC

    2016-06-01

    Full Text Available In-Chul Lee,1 Je-Won Ko,1 Sung-Hyeuk Park,1 Je-Oh Lim,1 In-Sik Shin,1 Changjong Moon,1 Sung-Hwan Kim,2 Jeong-Doo Heo,3 Jong-Choon Kim1 1College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University, Gwangju, 2Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup, 3Gyeongnam Department of Environment and Toxicology, Korea Institute of Toxicology, Gyeongnam, Republic of Korea Abstract: Despite widespread use and prospective biomedical applications of copper nanoparticles (Cu NPs, their biosafety issues and kinetics remain unclear. Thus, the aim of this study was to compare the detailed in vivo toxicity of Cu NPs and cupric ions (CuCl2; Cu ions after a single oral dose. We determined the physicochemical characteristics of Cu NPs, including morphology, hydrodynamic size, zeta potential, and dissolution in gastric (pH 1.5, vehicle (pH 6.5, and intestinal (pH 7.8 conditions. We also evaluated the kinetics of Cu following a single equivalent dose (500 mg/kg of Cu NPs and Cu ions. Cu NPs had highest dissolution (84.5% only in gastric conditions when compared with complete dissolution of Cu ions under various physiological milieus. Kinetic analysis revealed that highest Cu levels in blood and tested organs of Cu NP-treated rats were 15%–25% lower than that of Cu ions. Similar to the case of Cu ions, Cu levels in the tested organs (especially liver, kidney, and spleen of Cu NP-treated rats increased significantly when compared with the vehicle control. However, delay in reaching the highest level and biopersistence of Cu were observed in the blood and tested organs of Cu NP-treated rats compared with Cu ions. Extremely high levels of Cu in feces indicated that unabsorbed Cu NPs or absorbed Cu ions were predominantly eliminated through liver/feces. Cu NPs exerted apparent toxicological effects at higher dose levels compared with Cu ions and showed sex-dependent differences in mortality, biochemistry, and

  3. Jovens e experimentações da violência no brasil: estética da destruição, sociabilidades limítrofes

    Directory of Open Access Journals (Sweden)

    Rosamaria Luiza de Melo Rocha

    2000-10-01

    Full Text Available El artículo aborda algunos fenómenos de violencia en ciudades brasileñas, desde un punto de vista comunicacional; la autora logra identificar intersecciones entre ellos, particularmente frente a aspectos de visibilidad, construcción de identidad y sociabilidad que involucran a jóvenes urbanos.Buscando sintetizar reflexiones acerca de ciertas ocurrencias de la violencia –acto social y simbólico- en tal contexto, centra su preocupación en la experimentación de una “cultura do risco” – desafiante, personalista y a veces letal.Propone, además, que la violencia se manifiesta y puede ser percibida bajo un proceso dinámico y dialógico fundado en originales ordenaciones sociales y visuales. Postula, finalmente, que la violencia hoy constituye un poderoso lenguaje, una pedagogía percibida e “incorporada” por diversos sectores juveniles.Este por demás inquietante componente de la comunicación urbana, termina poniendo en evidencia lugares de construcción identitaria a veces tensionados y explosivos, a veces estetizados, poéticos, demarcados por la creciente simbiosis media/sociedad, por la vivencia de los media en cuanto nuevo espacio público y de las ciudades en cuanto peculiares escenarios y focos preferenciales de la visibilidadmediatizada.The article approaches the phenomena of violence in Brazilian cities from a communicational perspective; the author is able to identify intersections between these phenomena, particularly what they have to do with aspects of visibility and construction of identity and sociability as these concepts relate to urban youth.Looking to summarize reflections about certain occurences of violence — a social and symbolic act –- in that context, the author focuses her concern on the experimentation of a “culture of risk,” which is challenging, personalist, and sometimes lethal.In addition, she proposes that violence manifests itself and can be perceived in a dynamic and dialogic process founded on original social and visual orderings. Finally, she postulates that violence today constitutes a powerful language, a pedagogy perceived and “incorporated” by diverse sectors of youth.This very perturbing component of urban communication shows places of construction of identity that are sometimes tense and explosive, sometimes aesthetized, poetic, delimited by the growing media-society symbiosis, by personal experience with the media as a new public space and the cities as peculiar scenarios and preferential focuses of mass media visibility.

  4. Hyperosmotic stress induces Rho/Rho kinase/LIM kinase-mediated cofilin phosphorylation in tubular cells: key role in the osmotically triggered F-actin response

    DEFF Research Database (Denmark)

    Thirone, Ana C P; Speight, Pam; Zulys, Matthew

    2009-01-01

    Hyperosmotic stress induces cytoskeleton reorganization and a net increase in cellular F-actin, but the underlying mechanisms are incompletely understood. While de novo F-actin polymerization likely contributes to the actin response, the role of F-actin severing is unknown. To address this proble...... in the hyperosmotic stress-induced F-actin increase. Key words: cytoskeleton, hypertonicity, cell volume, small GTPases.......Hyperosmotic stress induces cytoskeleton reorganization and a net increase in cellular F-actin, but the underlying mechanisms are incompletely understood. While de novo F-actin polymerization likely contributes to the actin response, the role of F-actin severing is unknown. To address this problem...... we investigated whether hyperosmolarity regulates cofilin, a key actin-severing protein, whose activity is inhibited by phosphorylation. Since the small GTPases Rho and Rac are sensitive to cell volume changes, and can regulate cofilin phosphorylation, we also asked if they might link osmostress...

  5. The p53 co-activator Zac1 neither induces cell cycle arrest nor apoptosis in chicken Lim1 horizontal progenitor cells.

    Science.gov (United States)

    Fard, S Shirazi; Blixt, Mke; Hallböök, F

    2015-01-01

    Chicken horizontal progenitor cells are able to enter their final mitosis even in the presence of DNA damage despite having a functional p53-p21 system. This suggests that they are resistant to DNA damage and that the regulation of the final cell cycle of horizontal progenitor cells is independent of the p53-p21 system. The activity of p53 is regulated by positive and negative modulators, including the zinc finger containing transcription factor Zac1 (zinc finger protein that regulates apoptosis and cell cycle arrest). Zac1 interacts with and enhances the activity of p53, thereby inducing cell cycle arrest and apoptosis. In this work, we use a gain-of-function assay in which mouse Zac1 (mZac1) is overexpressed in chicken retinal progenitor cells to study the effect on the final cell cycle of horizontal progenitor cells. The results showed that overexpression of mZac1 induced expression of p21 in a p53-dependent way and arrested the cell cycle as well as triggered apoptosis in chicken non-horizontal retinal progenitor cells. The negative regulation of the cell cycle by mZac1 is consistent with its proposed role as a tumour-suppressor gene. However, the horizontal cells were not affected by mZac1 overexpression. They progressed into S- and late G2/M-phase despite overexpression of mZac1. The inability of mZac1 to arrest the cell cycle in horizontal progenitor cells support the notion that the horizontal cells are less sensitive to events that triggers the p53 system during their terminal and neurogenic cell cycle, compared with other retinal cells. These properties are associated with a cell that has a propensity to become neoplastic and thus with a cell that may develop retinoblastoma.

  6. Óleo essencial de limão no ensino da cromatografia em camada delgada Lime essential oil in the teaching of thin layer chromatography

    Directory of Open Access Journals (Sweden)

    Rosaly S. Silva

    2009-01-01

    Full Text Available This paper describes a simple experiment employing the essential oil of limes which can be applied in undergraduate organic chemistry laboratory classes for the teaching of thin layer chromatography (TLC. The experiment consists in submit lime peel oil to TLC separation employing hexane and dichloromethane as the eluents and five different systems for visualization of the chromatogram. In one experiment it is possible to teach the different variables of the TLC technique. This experiment may also be performed following vapor distillation and liquid-liquid extraction technique in experimental classes.

  7. Navigating the al- baḥr al- muẓlim: an Assessment of the Investigation, Mitigation and Preservation of Morocco's Maritime Cultural Heritage

    Science.gov (United States)

    Trakadas, Athena

    2012-10-01

    Despite the Kingdom of Morocco's extensive coastline and strategic position between the Mediterranean Sea and Atlantic Ocean, study of the region's maritime history has been limited. Recent archaeological investigations, however, reveal the great promise of the physical remains and historical depth of the region's maritime cultural heritage. As is the case with other countries in Africa, there are critical factors that adversely affect not only the exploration of this heritage, but also its recognition and preservation. Against this background, the Oued Loukkos Survey was developed in order to mitigate such critical factors at the site of Lixus (Larache) by implementing a cross-disciplinary methodology and cooperation between several Moroccan and foreign institutions. As Morocco recently ratified the UNESCO 2001 Convention on the Protection of the Underwater Cultural Heritage, a more comprehensive legal framework is now in place alongside existing national legislation for such mitigation practices to be enacted on a broader scale.

  8. The LIM homeobox transcription factor Lhx2 is required to specify the retina field and synergistically cooperates with Pax6 for Six6 trans-activation.

    Science.gov (United States)

    Tétreault, Nicolas; Champagne, Marie-Pier; Bernier, Gilbert

    2009-03-15

    In mammals, a limited set of homeobox-containing transcription factors are expressed in the presumptive eye field and required to initiate eye development. How these factors interact together at the genetic and molecular level to coordinate this developmental process is poorly understood. We found that the Lhx2 and Pax6 transcription factors operate in a concerted manner during retinal development to promote transcriptional activation of the Six6 homeobox-gene in primitive and mature retinal progenitors. Lhx2 demarcates the presumptive retina field at the neural plate stage and Lhx2 inactivation delays initiation of Rx, Six3 and Pax6 expression in this domain. The later expressed Six6 is properly activated in the pituitary/hypothalamic axis of Lhx2(-/-) embryos, but expression fails to be initiated in the optic vesicle. Lhx2 and Pax6 associate with the chromatin at several regions of Six6 in vivo and cooperate for trans-activation of Six6 regulatory elements in vitro. In retinal progenitor/stem cells, both Lhx2 and Pax6 are genetically required for proper Six6 expression and forced co-expression of Lhx2 and Pax6 can synergistically trans-activate the Six6 locus. Our work reveals how two master regulators of eye development coordinate their action to sequentially promote tissue-specific transcriptional initiation and full activation of a retinal determinant gene.

  9. Automated washing of FTA Card punches and PCR setup for reference samples using a LIMS-controlled Sias Xantus automated liquid handler

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Olsen, Addie Nina; Frøslev, Tobias G.;

    2009-01-01

    We have implemented and validated automated methods for washing FTA Card punches containing buccal samples and subsequent PCR setup using a Sias Xantus automated liquid handler. The automated methods were controlled by worklists generated by our LabWare Laboratory Information Management System (L...

  10. Respuesta fisiológica y bioquímica de plantas de limón mexicano infectadas con Candidatus Liberibacter asiaticus

    OpenAIRE

    Soto Leal, Mónica Gisela

    2014-01-01

    Actualmente la enfermedad HLB está afectando seriamente la citricultura nacional. Hasta la fecha se ha reportado a Ca. Liberibacter spp. como el agente causal de la enfermedad HLB. La mayoría de las investigaciones se han basado en detectar al patógeno, ya que la principal limitante es que éste patógeno no se puede cultivar in vitro, los estudios sobre la respuesta fisiológica y bioquímica de la planta ante la infección por Ca. Liberibacter asiaticus son escasos. Por lo que, el objetivo de la...

  11. Oma rahva säästmine on riigi ülim ülesanne / Aleksandr Solzhenitsõn ; interv. Vitali Tretjak

    Index Scriptorium Estoniae

    Solženitsõn, Aleksandr, 1918-2008

    2006-01-01

    Venemaa nädalalehes Moskovskije Novosti ilmunud vene ühiskondliku mõtte korüfee Aleksandr Solzhenitsõni intervjuu, mis analüüsib olukorda maailmas ja Venemaal. Vt. samas: Ilmar Mikiver. Solzhenitsõn - apostaat?; Aleksandr Solzhenitsõn Eestis

  12. LIM kinase 1 (LIMK1) interacts with tropomyosin-related kinase B (TrkB) and Mediates brain-derived neurotrophic factor (BDNF)-induced axonal elongation.

    Science.gov (United States)

    Dong, Qing; Ji, Yun-Song; Cai, Chang; Chen, Zhe-Yu

    2012-12-07

    BDNF/TrkB signaling plays critical roles in axonal outgrowth of neurons, the process of which requires the remodeling of the cytoskeleton structure, including microtubules and filamentous actin. However, the mechanism by which BDNF/TrkB signaling regulates cytoskeleton reorganization is still unclear. Here, we identified a novel interaction between LIMK1 and TrkB, which is required for the BDNF-induced axonal elongation. We demonstrated that BDNF-induced TrkB dimerization led to LIMK1 dimerization and transphosphorylation independent of TrkB kinase activity, which could further enhance the activation and stabilization of LIMK1. Moreover, activated LIMK1 translocated to the membrane fraction and phosphorylated its substrate cofilin, thus promoting actin polymerization and axonal elongation. Our findings provided evidence of a novel mechanism for the BDNF-mediated signal transduction leading to axonal elongation.

  13. ‘AMALIYYAH TA’LIM HIFDH AL-MUFRADAT BI WASITHAH AL-UGHNIYAH LI TARJAMAH KUTUB AT-TURATS

    Directory of Open Access Journals (Sweden)

    Miftahul Ula

    2017-04-01

    Full Text Available The basic concept of Arabic language learning is learning kinds of noun. Therefore, it is necessary for Arabic language learners to know the name of things needed in communication. One of the causes of failure and success in language learning is the use of media. There are so many teaching media used in language learning, including songs. In this case, the teacher chooses the most favorite song of the students, and then changes its lyrics with difficult words of the classic book. This media is used because it is supposed to be able to create fun and enjoyable situation and give the students ease in memorizing new vocabularies, especially in understanding the meaning of the classic books. On the other hand, the weakness of this media is that the teacher, especially those who have lack of singing abilities, found any difficulties in preparing the vocabulary of the classic book and put them into the song lyrics.

  14. Eesti mehe välimääraja / Jaana Nõu, Siret Liivamägi, Katrin Õun, Silja Kalda ; intervjueerinud Tiina Kruus

    Index Scriptorium Estoniae

    2011-01-01

    Jaana Nõu, Siret Liivamägi, Katrin Õun ja Silja Kalda otsustavad nelja mehe foto põhjal nende ameti, hobide ja vanuse üle. Meesteks on anestesioloogia ja intensiivravi resident Arie Passov, Rocca al Mare kooli geograafia, ajaloo ja riigikaitse õpetaja Marek Nisuma, Põhja-Eesti päästekeskuse Lilleküla keskkomando päästja Martin Ringo ja Tallinna Ülikooli Rahvusvaheliste ja Sotsiaaluuringute Instituudi teadur Marti Taru

  15. Archetype Criticism of Rain Image in Wai-lim Yip's Poem%叶维廉诗歌中雨意象的原型批评

    Institute of Scientific and Technical Information of China (English)

    戴芝兰

    2008-01-01

    "雨"作为一个原始的古典意象,潜藏着复杂微妙的"心象"内容.当叶维廉早期的诗歌和都不约而同以"雨"这个原始意象结束全篇开始,"雨"几乎变成了叶维廉诗歌中极富意义的语词,显示了原始意象融贯古今震撼心灵的神秘力量.

  16. [Proposal and preliminary validation of a check-list for the assessment of occupational exposure to repetitive movements of the upper lims].

    Science.gov (United States)

    Colombini, D; Occhipinti, E; Cairoli, S; Baracco, A

    2000-01-01

    Over the last few years the Authors developed and implemented, a specific check-list for a "rapid" assessment of occupational exposure to repetitive movements and exertion of the upper limbs, after verifying the lack of such a tool which also had to be coherent with the latest data in the specialized literature. The check-list model and the relevant application procedures are presented and discussed. The check-list was applied by trained factory technicians in 46 different working tasks where the OCRA method previously proposed by the Authors was also applied by independent observers. Since 46 pairs of observation data were available (OCRA index and check-list score) it was possible to verify, via parametric and nonparametric statistical tests, the level of association between the two variables and to find the best simple regression function (exponential in this case) of the OCRA index from the check-list score. By means of this function, which was highly significant (R2 = 0.98, p check-list score which better corresponded to the critical values (for exposure assessment) of the OCRA index looked for. The following correspondance values between OCRA Index and check-list were then established with a view to classifying exposure levels. The check-list "critical" scores were established considering the need for obtaining, in borderline cases, a potential effect of overestimation of the exposure level. On the basis of practical application experience and the preliminary validation results, recommendations are made and the caution needed in the use of the check-list is suggested.

  17. Fluctuación poblacional de Diaphorina citri Kuwayana en limón persa (Citrus latifolia Tanaka) en la sabana de Huimanguillo, Tabasco.

    OpenAIRE

    García Garduza, Diógenes

    2012-01-01

    Diaphorina citri Kuwayama es el vector de la enfermedad Huanglongbing, la cual se encuentra actualmente en 14 de los 23 estados productores de cítricos en México. El estado de Tabasco, donde se cultivan 15, 406 ha de estos frutales, aún no es afectado por la enfermedad, por lo cual es importante tomar medidas para controlar las poblaciones del vector, cuya presencia en dicho estado fue detectada en 2005. El objetivo de este trabajo fue determinar la fluctuación poblacional de D. citri y la r...

  18. Image processing techniques for lemons and tomatoes classification Técnica de processamento de imagem para classificação de limões e tomate

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Loureiro Lino

    2008-01-01

    Full Text Available Vegetable quality is frequently referred to size, shape, mass, firmness, color and bruises from which fruits can be classified and sorted. However, technological by small and middle producers implementation to assess this quality is unfeasible, due to high costs of software, equipment as well as operational costs. Based on these considerations, the proposal of this research is to evaluate a new open software that enables the classification system by recognizing fruit shape, volume, color and possibly bruises at a unique glance. The software named ImageJ, compatible with Windows, Linux and MAC/OS, is quite popular in medical research and practices, and offers algorithms to obtain the above mentioned parameters. The software allows calculation of volume, area, averages, border detection, image improvement and morphological operations in a variety of image archive formats as well as extensions by means of "plugins" written in Java.A qualidade vegetal freqüentemente se refere a tamanho, forma, massa, firmeza, cor e danos, em que podem ser classificados e ordenados. Porém, sua implementação tecnológica se torna inviável, para pequenos e médios produtores, devido ao alto custo de softwares, equipamentos, além dos custos operacionais. Com base nessas considerações, a proposta deste trabalho é estudar a adaptação de um novo software, com código-fonte aberto para habilitar o sistema de classificação reconhecendo forma, volume, cor e possivelmente danos. O software chamado ImageJ, compatível com o Windows, Linux e MAC/OS, é bastante popular em práticas e pesquisas médicas, e oferece algoritmos para obter os parâmetros mencionados acima. Entre os recursos oferecidos pelo pacote destaca-se a disponibilidade de diversos algoritmos com código-fonte abertos para: manipulação dos mais variados formatos de arquivo de imagens, detecção de bordas, melhoria de imagens, cálculos diversos (áreas, médias, centróides e operações morfológicas. Este software disponibiliza também um ambiente gráfico que simplifica a utilização de tais recursos, além de permitir a extensão através de "plugins" escritos em Java.

  19. Oma rahva säästmine on riigi ülim ülesanne / Aleksandr Solzhenitsõn ; interv. Vitali Tretjak

    Index Scriptorium Estoniae

    Solženitsõn, Aleksandr, 1918-2008

    2006-01-01

    Venemaa nädalalehes Moskovskije Novosti ilmunud vene ühiskondliku mõtte korüfee Aleksandr Solzhenitsõni intervjuu, mis analüüsib olukorda maailmas ja Venemaal. Vt. samas: Ilmar Mikiver. Solzhenitsõn - apostaat?; Aleksandr Solzhenitsõn Eestis

  20. Cultivo experimental del ostión de mangle crassostrea rhizophorae (Guilding 1828) en el estero Vizcaya, Limón y Golfo de Nicoya, Costa Rica

    National Research Council Canada - National Science Library

    Jorge Alfaro Montoya

    2016-01-01

    ... que las láminas plásticas de techo (fibra de vidrio y resina) son las más apropiadas para la remoción masiva de ostras, y que el transporte en seco, con hielo, da rendimientos muy altos de ostras viables (93...

  1. Efecto de hongos micorrizogenos en dos patrones de cítricos limón rugoso, Citrus jambhiri, y mandarina cleopatra, Citrus reshni

    Directory of Open Access Journals (Sweden)

    Torres Héctor R.

    1989-12-01

    Full Text Available En un suelo ácido, a 1.100 msnm, se sembraron semillas de dos patrones de cítricos Citrus reshni y C. jambhiri conservadas en dos temperaturas 8 .10 0C y ambiente, almacenadas por diferentes tiempos. Plántulas de estos patrones se inocularon, al momento del trasplante a bolsas, con cepas micorrizógenas en suelo natural y suelo desinfestado. Se estableció una relación entre área foliar y el producto largo máximo por ancho máximo de las hojas. Con 30 días de almacenamiento se obtuvo el mayor porcentaje de emergencia, en temperaturas de 8-10° C se conservó más la semilla. En C. reshni las especies Acaulospora longulata + Entrophospora colombiana superaron a Glomus manihotis y al testigo en los dos tipos de suelo; en C. jambhiri en suelo natural la inoculación fue efectiva. Se encontraron coeficientes de determinación R2 mayores de 99% para ecuaciones de regresión lineal entre área foliar y largo x ancho de las hojas, y con muestras pequeñas se pudo estimar con exactitud el área foliar de patrones de cítricos en estado de vivero.Seeds of two citrus partners, Citrus reshni and C. jambhiri, preserved under two different temperatures-environmental temperature and temperature from 80 to 10 C were planted in an acid soil at 1.100 meters above sea level. In transplanting to bags, seedlings of these partners were inoculated with micorrhizal strains in a natural soil and in non-infected soil. A relationship among foliar area and the maximen length product by the maximum width in the leaves was established. The greatest emergency percentage was obtained during thirty days of storage; at temperatures from 8° to 10°C, the seed was better preserved. In C. reshni species Acaulospora longulata +Entrophospora colombiana exceeded Glomus manihotis and the control in both types of soil, in C. jambhiri in natural soil inoculation was effective. R2 over 99 % were found for linear regression among the foliar area and the length and width of the leaves. The accurate foliar area in Citrus partners in their first stages of growth was estimated through small samples.

  2. ANALISIS PENGARUH PENDAPATAN TERHADAP JUMLAH INFAQ PESERTA PENGAJIAN ROHANI Studi Kasus Majelis Ta’lim di Masjid Al-Aman, Sidoarum, Godean, Sleman

    Directory of Open Access Journals (Sweden)

    Zein Muttaqin

    2015-12-01

    Full Text Available Nowadays, there are growing religious study community that urges people to deepen religious teachings. This community appears as learning, warning and preparation  forum  to  face  trial  in  the  after  life.  It  is  assumed  that  by  following religious  community,  people  will  improved  their  understanding  about  religious teachings that will affected their life behavior. The purpose of this research is to analyze  the  effect  of  intensity  to  attend  religious  community,  income  and comprehension  toward  the  infaq  of  attendee  of  religious  community  and  to explain the infaq behavior of attendant of religious community. This  field  research  is  a  quantitative  research.  There  are  50  samples  that used in this research which consists by ladies. In this research, the writer is using primary  data  that  procured  by  questionnaire  distributions.  The  data  analysis technique  that  used  in  this  research  are  descriptive  data  analysis  and  multiple regression  analysis.  As  for  the  hypothesis  that  suggested  in  this  research  is  that intensity to attend religious community, income and comprehension are affecting the infaq of attendee of religious community. The  result  of  this  research  shows  that  intensity  to  attend  religious community (not affecting, income (affecting and comprehension (not affecting toward the infaq of attendee of religious community. Most of behavior that shown in this research is unconsciously by attendee as manifestation form God words in the  Quran  and  Sunnah.  Intensity,  income  and  comprehension  variables  are affecting  only  38,9  percent,  whereas  61,1  percent  is  affected  by  other  variables outside this research.

  3. Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression

    Directory of Open Access Journals (Sweden)

    Marta Barba

    2012-01-01

    Full Text Available Multipotent mesenchymal stem cells with extensive self-renewal properties can be easily isolated and rapidly expanded in culture from small volumes of amniotic fluid. These cells, namely, amniotic fluid-stromal cells (AFSCs, can be regarded as an attractive source for tissue engineering purposes, being phenotypically and genetically stable, plus overcoming all the safety and ethical issues related to the use of embryonic/fetal cells. LMP3 is a novel osteoinductive molecule acting upstream to the main osteogenic pathways. This study is aimed at delineating the basic molecular events underlying LMP3-induced osteogenesis, using AFSCs as a cellular model to focus on the molecular features underlying the multipotency/differentiation switch. For this purpose, AFSCs were isolated and characterized in vitro and transfected with a defective adenoviral vector expressing the human LMP3. LMP3 induced the successful osteogenic differentiation of AFSC by inducing the expression of osteogenic markers and osteospecific transcription factors. Moreover, LMP3 induced an early repression of the kruppel-like factor-4, implicated in MSC stemness maintenance. KLF4 repression was released upon LMP3 silencing, indicating that this event could be reasonably considered among the basic molecular events that govern the proliferation/differentiation switch during LMP3-induced osteogenic differentiation of AFSC.

  4. Estudio del comportamiento de hormonas vegetales y nutrientes durante el crecimiento y maduraci??n del lim??n (Citrus limonum Risso, cv. Verna)

    OpenAIRE

    1993-01-01

    Se ha estudiado el efecto de tratamientos ex??genos con ga3 y kinetina sobre el comportamiento de aba (t-aba y aba) y ga3 tanto en frutos como en hojas de limonero verna a lo largo de su ciclo vegetativo. Ambos tratamientos han llevado a una menor abscisi??n de frutos, uno de los principales problemas de este cultivo, debido al mayor contenido de ga3 durante el periodo de fijaci??n, a la tendencia descendente de los dos is??meros del aba y a la mayor acumulaci??n de prote??nas totales y nutri...

  5. Inserción laboral de migrantes limítrofes en el sector de la construcción (CABA)

    OpenAIRE

    Paoletti, María Eugenia

    2014-01-01

    Desde el Acta de Asunción del 24 de Marzo de 1991, en el que se manifiesta la expansión de las economías nacionales a nivel regional, con ampliación de los mercados y la libertad de circulación de capitales, bienes, servicios y personas hasta hoy, muchos han sido los intentos en la región para alcanzar acuerdos en normas comunes para que, entre otros derechos civiles, en el plano laboral, se establezca el derecho a realizar cualquier actividad, por cuenta propia o ajena en igualdad de condici...

  6. Revisiting the dissociation between singing and speaking in expressive aphasia.

    Science.gov (United States)

    Hébert, Sylvie; Racette, Amélie; Gagnon, Lise; Peretz, Isabelle

    2003-08-01

    We investigated the production of sung and spoken utterances in a non-fluent patient, C.C., who had a severe expressive aphasia following a right-hemisphere stroke, but whose language comprehension and memory were relatively preserved. In experiment 1, C.C. repeated familiar song excerpts under four different conditions: spoken lyrics, sung lyrics on original melody, lyrics sung on new but familiar melody and melody sung to a neutral syllable "la". In experiment 2, C.C. repeated novel song excerpts under three different conditions: spoken lyrics, sung lyrics and sung-to-la melody. The mean number of words produced under the spoken and sung conditions did not differ significantly in either experiment. The mean number of notes produced was not different either in the sung-to-la and sung conditions, but was higher than the words produced, hence showing a dissociation between C.C.'s musical and verbal productions. Therefore, our findings do not support the claim that singing helps word production in non-fluent aphasic patients. Rather, they are consistent with the idea that verbal production, be it sung or spoken, result from the operation of same mechanisms.

  7. Experiment list: DRX020614 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available all cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC)...totalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020614.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DR...X020614.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020614.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020614.20.bed http://dbarchive.biosciencedbc.jp/kyu

  8. Experiment list: DRX020616 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ll cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC) ...otalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020616.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DRX...020616.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020616.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020616.20.bed http://dbarchive.biosciencedbc.jp/kyu

  9. Experiment list: DRX020608 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ll cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC) ...otalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020608.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DRX...020608.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020608.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020608.20.bed http://dbarchive.biosciencedbc.jp/kyu

  10. Experiment list: DRX020615 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ll cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC) ...otalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020615.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DRX...020615.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020615.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020615.20.bed http://dbarchive.biosciencedbc.jp/kyu

  11. Experiment list: DRX020607 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ll cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC) ...otalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020607.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DRX...020607.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020607.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020607.20.bed http://dbarchive.biosciencedbc.jp/kyu

  12. Experiment list: DRX020604 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available all cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC)...totalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020604.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DR...X020604.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020604.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020604.20.bed http://dbarchive.biosciencedbc.jp/kyu

  13. Experiment list: DRX020610 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available all cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC)...totalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020610.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DR...X020610.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020610.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020610.20.bed http://dbarchive.biosciencedbc.jp/kyu

  14. Experiment list: DRX020606 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available all cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC)...totalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020606.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DR...X020606.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020606.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020606.20.bed http://dbarchive.biosciencedbc.jp/kyu

  15. Experiment list: DRX020605 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available all cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC)...totalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020605.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DR...X020605.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020605.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020605.20.bed http://dbarchive.biosciencedbc.jp/kyu

  16. Experiment list: DRX020613 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ll cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC) ...otalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020613.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DRX...020613.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020613.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020613.20.bed http://dbarchive.biosciencedbc.jp/kyu

  17. Experiment list: DRX020609 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available all cell carcinoma cell line:TC-YIK || sample_provider=Yukio Nakamura (RIKEN BRC)...totalRNA-miRNeasy_Mini-v1.0 || rna_od_260_230=1.85 http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bw/DRX020609.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/DR...X020609.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/DRX020609.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/DRX020609.20.bed http://dbarchive.biosciencedbc.jp/kyu

  18. Association between insulin and executive functioning in alcohol dependence: a pilot study

    Directory of Open Access Journals (Sweden)

    Han C

    2015-11-01

    Full Text Available Changwoo Han,1 Hwallip Bae,2 Sung-Doo Won,3 Jaeyoung Lim,3 Dai-Jin Kim41Department of Psychiatry, Ansan Hospital, College of Medicine, Korea University, Ansan, 2Department of Psychiatry, Myongji Hospital, College of Medicine, Seonam University, Goyang, 3Department of Clinical Psychology, Keyo Hospital, Keyo Medical Foundation, Uiwang, 4Department of Psychiatry, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaAbstract: Alcohol dependence is a disorder ascribable to multiple factors and leads to cognitive impairment. Given that insulin dysregulation can cause cognitive impairment, patients with alcohol dependence are likely to develop insulin dysregulation such as that in diabetes. The purposes of this study are to identify an association between cognitive functioning and insulin and to investigate insulin as the biomarker of cognitive functioning in alcohol-dependent patients. Serum insulin levels were measured and cognitive functions were assessed in 45 patients with chronic alcoholism. The Korean version of the Consortium to Establish a Registry for Alzheimer’s Disease (CERAD-K, a battery of cognitive function tests, was used to assess cognitive functioning. Serum insulin levels were not significantly correlated with most CERAD-K scores, but there was a significant negative correlation with scores on the Trail Making Test B, which is designed to measure executive functioning. Lower serum insulin levels were associated with slower executive functioning responses on the Trail Making Test B, suggesting that executive functioning may be in proportion to serum insulin levels. Thus, in patients with alcohol dependence, insulin level is associated with cognitive functioning. In addition, the present findings suggest that insulin level is a potential biomarker for determining cognitive functioning.Keywords: insulin, alcohol dependence, executive function, trail making test

  19. Prevalence of and risk factors for postoperative pulmonary complications after lung cancer surgery in patients with early-stage COPD

    Directory of Open Access Journals (Sweden)

    Kim ES

    2016-06-01

    Full Text Available Eun Sun Kim,1 Young Tae Kim,2 Chang Hyun Kang,2 In Kyu Park,2 Won Bae,1 Sun Mi Choi,1 Jinwoo Lee,1 Young Sik Park,1 Chang-Hoon Lee,1 Sang-Min Lee,1 Jae-Joon Yim,1 Young Whan Kim,1 Sung Koo Han,1 Chul-Gyu Yoo1 1Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, 2Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine, Seoul, Republic of Korea Purpose: This study aimed to investigate whether the prevalence of postoperative pulmonary complications (PPCs in patients with non-small-cell lung cancer (NSCLC is even higher in the early stages of COPD than in such patients with normal lung function and to verify the usefulness of symptom- or quality of life (QoL-based scores in predicting risk for PPCs.Patients and methods: Patients undergoing pulmonary resection for NSCLC between July 2012 and October 2014 were prospectively enrolled. Preoperative measurements of lung function, dyspnea, and QoL, operative characteristics, PPCs, duration of postoperative hospitalization, and in-hospital mortality were assessed.Results: Among 351 consecutive patients with NSCLC, 343 patients with forced expiratory volume in 1 second (FEV1 ≥70% of predicted value were enrolled. At least one PPC occurred in 57 (16.6% patients. Prevalence of PPC was higher in patients with COPD (30.1% than in those with normal spirometry (10.0%; P<0.001. However, in patients with COPD, the prevalence of PPC was not different in patients with FEV1 ≥70% compared to those with FEV1 <70% and between group A (low risk and less symptoms and group B (low risk and more symptoms patients with COPD, based on the new Global initiative for chronic Obstructive Lung Disease 2011 guidelines. In patients with COPD, body mass index (odds ratio [OR]: 0.80, P=0.007, carbon monoxide diffusing capacity of the lung (DLCO, % predicted value (OR: 0.97, P=0.024, and operation time (OR: 1.01, P=0.003, but not COPD assessment test or St

  20. Systematik zur Gestaltung und Optimierung von wissensintensiven, kooperativen Problemlösungsprozessen in der Produktentwicklung

    OpenAIRE

    Wagner, Kristina

    2008-01-01

    Das Ziel der vorliegenden Arbeit war es, die verteilte Lösung von wissensintensiven Problemen in den frühen Phasen der Produktentwicklung zu unterstützen. Dies umfasst sowohl individuelle als auch kooperative Aspekte der Problemlösung. Im Vordergrund stand dabei die Betrachtung der Aufbau- und Ablauforganisation, die Betrachtung der für die Problemlösung relevanten Wissens- und Lernprozesse und die Etablierung eines ganzheitlichen, integrierten Problemlösungsmanagement. Ferner galt es, realen...

  1. Investigation of Efficient CO Laser Frequency Tripling.

    Science.gov (United States)

    1979-09-01

    C: sueo 149 C sumG 151 1=1 SUNG 152 I"=? SUNG 154 1U22 SUNO 155 luz SUNG 1ss CALI. FRLO SUmG IS? Lz’ SUNO 1SS 10?Sumo 159 ICA? SUNG 160o lull WUHO 161...UPT22 FTN 4.b.’.3JO 1)1/01/78 09.32.25 C CH1IONE 59 CS DL8jiG CI4IONE 60 CS AH~qAYS CHIONE 61 CS GOTOS CHIONE 62 REAL LINEW(59% CINE 6 DIMENSION UfrESO

  2. The archives of solar integral exposures and of spectroheliograms of the former Fraunhofer Institute (now: Kiepenheuer Institute for Solar Physics) in Freiburg and its partial dissolution (German Title: Die Archive solarer Integralaufnahmen und von Spektroheliogrammen des früheren Fraunhofer-Instituts (jetzt: Kiepenheuer-Institut für Sonnenphysik) in Freiburg und ihre teilweise Auflösung)

    Science.gov (United States)

    Wöhl, Hubertus

    The former Fraunhofer-Institut which was founded about 60 years ago and since 1978 is named Kiepenheuer-Institut für Sonnenphysik (KIS), has been for several decades a center to collect information about solar activity. One of the reasons for interest in solar activity were in the beginning attempts to forecast disturbances of (military) radio communication caused by solar eruptions. This would today be called ‘space weather research’. Later daily maps of the sun - showing its activity - were edited for many years. The data needed to describe solar activity were gained mainly from photographic images: Since 1939 white light images of the full solar disk were collected and stored in the archive. Since 1943 in addition spectroheliograms of the full solar disk in H-alpha and in Ca II K3 were collected. These images were taken on glass plates (some on film) of sizes 9 x 12 cm in the case of the white light images and of sizes 6 x 12 cm in the case of the spectroheliograms and were stored in envelopes with additional information written on them. Several hundred of these plates in their envelopes were combined in open wooden boxes each. These boxes were stored in open shelfs in a meeting room of the old solar observatory on the Schauinsland mountain near Freiburg. Within the last years it became obvious that the quality of many plates stored was bad and that the possible scientific usage was becoming very limited. In summer 2002 I started to investigate the quality of the white light images and prepared a data base in MS ACCESS XP about the plates I found which were observed at more than 10 different observing places. The total number of plates checked was 11782. Depending on the quality and possible later usage for an investigation of proper motions in sunspot groups I kept several series of them. Most of the plates kept stem from the years 1945 until 1949 and 1955 until 1959 - when the solar activity was extremely high. In total about 2000 plates were kept. It is intended to digitize as many as possible of these selected plates using a high precision scanner for transparencies. Some examples of digitized images are already available. More details can be found in a text about the history of the Fraunhofer-Institut in the WWW pages of the KIS at: http://www.kis.uni-freiburg.de/kisgeschichte_e.html In summer 2003 I started to investigate the quality of the spectroheliograms on glass plates and to prepare another data base of the material I find in the archive.

  3. 專論/部落格使用者的資訊行為特徵研究/高世樺;林頌堅 | A Study of Features of Blog Users' Information Behaviors / Shih-Hua Kao; Sung-Chien Lin

    Directory of Open Access Journals (Sweden)

    高世樺、林頌堅

    2008-04-01

    Full Text Available

    部落格已經成為諸多新科技中最具有影響力的媒體之一,並對於使用者的資訊尋求行為造成影響。本研究整理部落格的相關概念及功能,並以二十位部落格使 用者的訪談結果對部落格上的資訊尋求行為特徵進行描述。研究結果顯示,使用者在部落格的開始動機、連結方式、辨別標準、監看與提取方式等等都展現了十分豐 富的行為特徵,研究並發現;(1情感聯繫是受訪者經常提及的使用目標,並且這種動機也影響了各種行為特徵;(2使用者會依照自我的需求以及所處的情境 發展出合適的使用方式,這些新使用方式可能不同於發明者與推廣者所設想的最便於使用的功能;(3部落格上的個人經驗是使用者進行資訊尋求的主要原因之 一;(4豐富而精緻的圖像經常能夠吸引使用者進入部落格。

    Blog has already become one of the most influential media among new technologies, and has impacted on users’ information seeking behaviors. This research  utilized an interview survey to explore related concepts and unique functions of blog media. Interviews with 20 blog users were completed and analyzed to depict characteristics of information seeking behaviors founded in the use of blogs. The result shows that the users had performed a variety of behavioral features in motivations of using blogs, ways of linking from one blog to another, criteria of identification among blogs, methods of blog monitoring and extracting and so forth. Some major observations were also found during the interviews conducted: (1 emotion connection is frequently mentioned by users as one of their main purposes of using blogs, which can be found in each characteristic of information seeking behaviors; (2 when users use new functions of blog, based on their respective needs and situations they can develop their own strategies and methods, which can be very different from those provided or assumed by inventors and promoters; (3 personal experience shared by bloggers is one of the primary reasons why users employ blogs to find information needed; (4 abundant and delicate images embedded in blogs can always attract users’ attention.

    頁次:62-77

  4. 有关韩国与中国大学生使用社交媒体的动机对社交媒体广告使用意向的影响之研究%A study on the effects and motives of college students using social media on advertisement usage between Korea and ChinaShim Sung Wook

    Institute of Scientific and Technical Information of China (English)

    沈星旭

    2011-01-01

    本文主要是为了研究韩国与中国的大学生使用社交媒体( Social Media)的动机,以及与广告使用动机进行比较并找出其差异.为此本文采用媒体的使用与满足理论(use and gratification theory),找出使用社交媒体的动机,并分析了这些动机对社交媒体广告的使用意向起到何种影响.分析结果显示,第一、韩国与中国的大学生使用社交媒体都是出于实用性动机;第二、在两国使用社交媒体的动机来看,韩国只有在使用值得信赖的广告方面起到积极影响,而中国在信息的可信赖度、转换心情、个人实用性等使用广告的三个动机上都起到了影响.本文的研究结果表明,在使用社交媒体广告时,韩国消费者的“使用网络信息与内容”、“网络搜索”、“追求娱乐”等使用动机起到了积极效果,而社交媒体广告的可信赖度得到满足最为重要;而中国消费者的“网络搜索”对使用“值得信赖的”广告的动机时起到了积极影响,“社会联系”、“在意别人对自己的评价”等选项则是为了“转换心情”使用广告起到了积极影响,另外“社会联系”还对“个人实用性”广告使用动机起到了影响.

  5. Experiment list: SRX472902 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ease=Normal 34903926,88.0,23.8,10479 GSM1330729: MRC5 RNAPII-2 hours; Homo sapiens; ChIP-Seq source_name=MRC...C5 || treatment=0.2mM H2O2 || tiime=2 hours http://dbarchive.biosciencedbc.jp/kyu

  6. In-Situ Neutron Diffraction Study of Aging of a Mg-Y-Nd-Zr Alloy (WE43): Effects of Precipitation on Individual Deformation Mechanisms

    Science.gov (United States)

    2012-07-01

    direct-chill cast by Magnesium Elektron UK in Swinton, Manchester, England. A block from the cast slab was provided for the present experiments...Laboratory under Cooperative Agreement No. W911NF-07-2-0073, as a subcontract with Magnesium Elektron North America with Mr. Rick Delorme and Dr. Kyu Cho

  7. Experiment list: SRX277329 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available me=Murine erythroleukemia (MEL) cells || cell line=non-induced C88 MEL cells || chip antibody=CDK9 C20 antibody (Santa Cruz Biotechno...logy, C20, sc-484) http://dbarchive.biosciencedbc.jp/kyu

  8. Experiment list: SRX673716 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available down of blrp-tagged proteins=Biotin-ChIP using Streptavidin Magnetic beads (Solulink) || treatment=24hrs treatment with 2μg/ml doxycy...cline, 1hr treatment with 1μM RA and 100nM E2 http://dbarchive.biosciencedbc.jp/kyu

  9. Experiment list: SRX1008304 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available 4me3 (07-473, Millipore,Billerica, MA, USA) http://dbarchive.biosciencedbc.jp/kyu...train=B6129SF1 crossed with C57BL/6 || cell type=Fetal Liver, Lineage-Sca1+c-Kit+ (LSK) || chip antibody=H3K

  10. Experiment list: DRX012366 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available GATA3WT antiFlag? sample_name=DRS012118 || sample comment=3xFlag-tagged Gata3-expressing mouse Th2 cell clone, D10G4.1 cells weresubj...ected to ChIP-seq analysis for anti-Flag mAb. http://dbarchive.biosciencedbc.jp/kyu

  11. Experiment list: SRX425484 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ody (M70, Santa Cruz Biotechnologies, Inc.) http://dbarchive.biosciencedbc.jp/kyu...4 cells at day 0 || cell type=preosteoblast || passages=28-31 || strain=C57BL/6 || chip antibody=Runx2 antib

  12. lim(∞+∞+…+∞)1/∞型极限计算

    Institute of Scientific and Technical Information of China (English)

    赵建红

    2014-01-01

    将命题lim n→∞(a1n+a2n+…+amn)1/n=max{a1,a2,…,am}推广到函数极限上可得:lim x→+∞(f1x(x)+f2x(x)+…+fmx(x))1/x=max{lim x→+∞f1(x),lim x→+∞f2(x),…,lim x→+∞fm(x)}和lim x→x0(f1φ(x)(x)+f2φ(x)(x)+…+fmφ(x)(x))1/φ(x)=max{lim x→x0f1(x),lim x→x0f2(x),…,lim x→x0fm(x)}等.

  13. The Tonal Function of a Task-Irrelevant Chord Modulates Speed of Visual Processing

    Science.gov (United States)

    Escoffier, N.; Tillmann, B.

    2008-01-01

    Harmonic priming studies have provided evidence that musical expectations influence sung phoneme monitoring, with facilitated processing for phonemes sung on tonally related (expected) chords in comparison to less-related (less-expected) chords [Bigand, Tillmann, Poulin, D'Adamo, and Madurell (2001). "The effect of harmonic context on phoneme…

  14. Music training and rate of presentation as mediators of text and song recall.

    Science.gov (United States)

    Kilgour, A R; Jakobson, L S; Cuddy, L L

    2000-07-01

    The present research addresses whether music training acts as a mediator of the recall of spoken and sung lyrics and whether presentation rate is the essential variable, rather than the inclusion of melody. In Experiment 1, 78 undergraduates, half with music training and half without, heard spoken or sung lyrics. Recall for sung lyrics was superior to that for spoken lyrics for both groups. In Experiments 2 and 3, presentation rate was manipulated so that the durations of the spoken and the sung materials were equal. With presentation rate equated, there was no advantage for sung over spoken lyrics. In all the experiments, those participants with music training outperformed those without training in all the conditions. The results suggest that music training leads to enhanced memory for verbal material. Previous findings of melody's aiding text recall may be attributed to presentation rate.

  15. Experimental Analysis of Linear Induction Motor under Variable Voltage Variable Frequency (VVVF Power Supply

    Directory of Open Access Journals (Sweden)

    Prasenjit D. Wakode

    2016-07-01

    Full Text Available This paper presents the complete analysis of Linear Induction Motor (LIM under VVVF. The complete variation of LIM air gap flux under ‘blocked Linor’ condition and starting force is analyzed and presented when LIM is given VVVF supply. The analysis of this data is important in further understanding of the equivalent circuit parameters of LIM and to study the magnetic circuit of LIM. The variation of these parameters is important to know the LIM response at different frequencies. The simulation and application of different control strategies such as vector control thus becomes quite easy to apply and understand motor’s response under such strategy of control.

  16. Inhaled indacaterol for the treatment of COPD patients with destroyed lung by tuberculosis and moderate-to-severe airflow limitation: results from the randomized INFINITY study

    Directory of Open Access Journals (Sweden)

    Kim CJ

    2017-05-01

    Full Text Available Cheong-Ju Kim,1 Hyoung-Kyu Yoon,2 Myung-Jae Park,3 Kwang-Ha Yoo,4 Ki-Suck Jung,5 Jeong-Woong Park,6 Seong Yong Lim,7 Jae Jeong Shim,8 Yong Chul Lee,9 Young-Sam Kim,10 Yeon-Mok Oh,11 Song Kim,12 Chul-Gyu Yoo13 1Department of Internal Medicine, National Health Insurance System Ilsan Hospital, Koyang, 2Division of Pulmonology, Department of Internal Medicine, St Mary’s Hospital, College of Medicine, The Catholic University of Korea, 3Division of Respiratory and Critical Care Medicine, Kyung Hee University Hospital, Kyung Hee University, Seoul, 4Department of Internal Medicine, Konkuk University School of Medicine, Gwangjin-gu, 5Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Hallym University Sacred Heart Hospital, Hallym University Medical School, Anyang-si, 6Division of Pulmonary and Allergy Medicine, Gachon University Gil Medical Center, Incheon, 7Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, 8Department of Internal Medicine, Korea University College of Medicine, Seoul, 9Department of Internal Medicine and Research Center for Pulmonary Disorders, Chonbuk National University Medical School, Jeonbuk, 10Department of Internal Medicine, Yonsei University College of Medicine, 11Department of Pulmonary and Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, 12Clinical Development and Medical Affairs, Novartis Korea Ltd., Seoul, 13Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea Background and objective: Pulmonary tuberculosis (TB is a risk factor for chronic obstructive pulmonary disease (COPD; however, few clinical studies have investigated treatment effectiveness in COPD patients with destroyed lung by TB. The Indacaterol effectiveness in COPD patients with Tuberculosis history (INFINITY

  17. Determinación de propiedades físicas y parámetros de transporte a colas auríferas de mina El Limón

    Directory of Open Access Journals (Sweden)

    J. Ramírez

    2006-01-01

    Full Text Available Los parámetros de transporte de colas mineras y otras propiedades físicas, tales como permeabilidad intrínseca, distribución de tamaño de partículas, porosidad y densidad fueron determinados experimentalmente. Se realizaron experimentos de infiltración en columnas de laboratorio con el fin de determinar; la conductividad hidráulica, las propiedades capilares y estudiar el comportamiento hidrodinámico de las colas auríferas. También se hizo uso de un modelo matemático para simular la infiltración isotérmica unidimensional y ajustar los perfiles experimentales, con el propósito de determinar los parámetros de transporte que describen el proceso de infiltración. Aunque los experimentos se realizaron con diferentes grados de compactación, se obtuvieron parámetros con una coherencia razonable para describir la conductividad hidráulica y las propiedades capilares de las colas por este método. Estos parámetros fueron usados en un modelo simple para simular el lavado con agua del cianuro contenido en las colas auríferas.

  18. 北京地铁机场线直线电机国产化应用研究%Localization Research on LIM for the Airport Line of Beijing Subway

    Institute of Scientific and Technical Information of China (English)

    崔霆锐; 宗立明; 李熙; 孟磊

    2016-01-01

    The motor was optimized independently from both electromagnetic properties and process structures. Static and dynamic experiments were conducted on the two trial domestic motors which were installed in a vehicle of airport line. The results revealed that technical parameters of both domestic and imported motor were basically the same, that traction performance and electrical properties were quite, and that the thermal property and efficacy of the domestic motor was superior to the imported one. On this basis, the two domestic motors were tested in a passenger operational assessment of one hundred thousand kilometers. So the adaptability of the domestic motor to actual operating environment in airport line had been verified. And the domestic product was proved an alternative to the imported one.%从电磁性能和工艺结构两方面对电机进行自主优化设计,并将试制的2台国产电机安装在北京地铁机场线车辆上进行静动态调试试验. 试验结果表明国产电机各项技术参数与进口电机基本一致,牵引性能及电气性能与进口电机相当,在温升特性和功效等方面有所提升. 在此基础上又对2台国产电机进行了十万公里载客运营考核试验,验证了国产电机对北京地铁机场线实际运营环境的适应性,以及对同类进口产品的替代性.

  19. 利用实验室信息管理系统创建合并院校的数字化实验室%Creation of the Digital Laboratories in Incorporated Universities with LIMS

    Institute of Scientific and Technical Information of China (English)

    刘思周

    2004-01-01

    本文通过对实验室信息管理系统(Laboratory Information Management System)中专用分析方法、GIS(地理信息系统)、宽带网络服务、仪器设备远程控制和数据集中处理与共享等主要特性的介绍,阐述了这一系统对于解决合并院校实验室建设与管理中跨学科、跨地域管理实验项目、人员、资金、物资、仪器设备、资料情报和数据等的重要意义.指出这一系统是合并院校依照教育部建立高效、规范、严密、低运行成本的数字化实验室管理制度的强大工具.

  20. 应用于LIMS系统的RS232串行通讯端口程序设计%Using in the programmer of RS232 com port of LIMS

    Institute of Scientific and Technical Information of China (English)

    张宏伟; 邹尔宁

    2005-01-01

    本论文的设计就是为了解决实验室中具有RS232标准接口的仪器与PC机之间的数据通信问题.使实验员们能够从繁杂的手工劳动中解脱出来.使企业能够适应信息时代的要求,充分体现出方便、快捷、准确的优越性.

  1. Composición química de la biomasa residual de la planta de piña variedad MD2 proveniente de Guácimo, Limón

    OpenAIRE

    Irías, Andrea; Lutz, Giselle

    2015-01-01

    Se determinó durante el mes de febrero del año 2009, el contenido de humedad, ligninas, holocelulosa, nitrógeno y minerales (potasio, calcio, hierro, magnesio, sodio) en la biomasa residual de la planta de piña variedad MD2 de la región de Guácimo, mediante los métodos extracción con disolventes, Kjeldahl y espectrometría de absorción atómica. Se observaron diferencias en la composición química reportada para cultivos de otras regiones, lo cual debe ser tomado en cuenta para el aprovechamient...

  2. Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD Poliembrionia e identificação de seedlings zigóticos e nucelares de limão volkameriano com RAPD

    Directory of Open Access Journals (Sweden)

    María Andrade-Rodríguez

    2004-06-01

    Full Text Available The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitrofor six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (POs objetivos deste trabalho foram avaliar a freqüência da poliembrionia e indentificar os seedlings zigóticos e nucelares de Citrus volkameriana usando RAPD. Cultivaram-se in vitro 25 sementes poliembriônicas e 8 monoembriônicas, por seis meses. Extraiu-se o DNA dos seedlings e usou-se em combinação com cinco primers RAPD para identificar a origem zigótica e nucelar das plântulas. Os resultados mostraram que as condições ambientais do ano afetaram significativamente (P<0,05 as características morfológicas dos frutos e o número de embriões por semente. Os índices de sementes poliembriônicas variaram nos três anos de avaliação (30,9%, 44,8% e 54,4%. As características morfológicas não se correlacionaram com a poliembrionia. A cultura in vitro possibilita que todos os embriões de cada semente cresçam, favorecendo os seedlings identificados como zigóticos. Nas sementes poliembriônicas e monoembriônicas, 25,9% e 87,5% dos seedlings, respectivamente, originaram-se sexualmente. Nem todos os seedlings zigóticos foram produzidos por embriões localizados no micrófilo das sementes poliembriônicas.

  3. Preliminary Study of Service Discovery in Internet of Things:Feasibility and Lim-itation of SOA%物联网服务发现初探:传统SOA的可行性和局限性

    Institute of Scientific and Technical Information of China (English)

    魏强; 金芝; 李戈; 李力行

    2013-01-01

    In IoT (Internet of things) based on SOA (service-oriented architecture), functionalities of devices are encapsulated as services with a unified and common interface to be provided to the outside world. However, due to the massive, mobility and highly resource-constrained natures of devices, the unreliability of wireless network in IoT, services provided by devices have different characteristics with traditional Web services, and existing Web service discovery approaches can’t satisfy the requirements of service discovery in IoT. This paper introduces some typical Web service discovery approaches firstly from two aspects: Web service discovery architecture and Web service matchmaking strategy. Then combining characteristics of service providing in IoT, it analyzes the problems of Web service discovery in IoT and possible solutions from four aspects: scalability, limited resource, heterogeneity and dynamic environment. Finally, it discusses some problems of Web service discovery in IoT which need be resolved.%  基于 SOA(service-oriented architecture)的物联网(Internet of things,IoT)把设备的功能服务化,以一种统一和通用的接口向外界提供服务。由于物联网中设备的海量性、移动性和资源高度受限性,以及无线网络自身的不可靠性,设备服务与传统的Web服务相比具有不同的特点,现有的Web服务发现方法不能有效地满足物联网中服务发现的需求。从Web服务发现体系结构和匹配策略两个方面对典型的Web服务发现方法进行了分析;结合物联网中服务提供的特点,从可扩展性、资源有限性、异构性和环境的动态变化性四个方面,分析了将Web服务发现方法应用于物联网服务提供中所面临的问题,并讨论了可能的解决思路;探讨了物联网中服务发现需要解决的问题。

  4. La percepción como herramienta para la gestión del riesgo : aportes para la cogestión comunitaria, caso de la comunidad de Sixaola, Limón, Costa Rica

    Directory of Open Access Journals (Sweden)

    Aguilar Fonseca, Maricarmen

    2013-12-01

    Full Text Available El presente artículo intenta posicionar a la percepción del riesgo como una de las principales herramientas por medio de las cuales se puede trabajar la gestión del riesgo en una comunidad. Esto porque para que exista un verdadero involucramiento de las y los actores comunitarios, se debe partir de lo que las personas conocen y entienden por riesgo, además de la forma en que se visualizan dentro del proceso de construcción de su propio riesgo This article tries to present risk perception as one of the main tools by means of which risk management in a community can be worked. Because for real involvement of communitarian actors to exist, is necessary to start from what people know and understand by risk, besides the way in which they visualize themselves within their own risk construction process

  5. Afección de suelos agrícolas por metales pesados en áreas limítrofes a explotaciones mineras del Sureste de España

    Directory of Open Access Journals (Sweden)

    F. Belmonte Serrato

    2010-01-01

    Full Text Available Se analiza la contaminación por metales en suelos agrícolas en el entorno de la Sierra minera de Cartagena-La Unión. El muestreo se realizó cogiendo 20 muestras de suelo en uso agrícola mediante una distribución aleatoria en un área de unos 100 km2 en torno a la Sierra Minera. Los resultados han detectado concentraciones importantes, que alcanzan y superan los niveles máximos permitidos por diversas normativas internacionales de hasta 11 de los elementos denominados «metales pesados». Aluminio y Hierro destaca sobre los demás con concentraciones medias porcentuales de 13% y 10% respectivamente. Pero hay que destacar la contaminación excesiva de plomo (Pb y Zinc (Zn que duplica e incluso triplica el máximo establecido por las leyes más permisivas, superando con mucho los niveles máximos a partir de los cuales se requiere una intervención obligatoria en todas las legislaciones consultadas.

  6. Pobreza estructural y migración limítrofe: aportes para pensar su articulación espacial en la Aglomeración Gran Buenos Aires

    Directory of Open Access Journals (Sweden)

    Mariana Marcos

    2010-12-01

    Full Text Available Las formas que adquiere la distribución espacial de los grupos en ámbitos urbanos constituyen una preocupación de larga data en las ciencias sociales. En esta línea, el nivel socioeconómico y la condición migratoria son variables frecuentemente utilizadas por los estudios interesados en entender cómo y porqué se produce una localización diferencial de los grupos sociales al interior del espacio urbano. El presente trabajo se propone analizar la distribución espacial de la población residente en la Aglomeración Gran Buenos Aires, explorando la articulación entre la diferenciación por nivel socioeconómico y condición migratoria. Se realiza un análisis estadístico-cartográfico por fracciones censales en base a datos del Censo de Población, Hogares y Viviendas 2001, buscando indagar en qué medida se intersectan la dimensión socioeconómica y la migratoria en los procesos de distribución y diferenciación espacial a nivel intraurbano.

  7. Joint swelling

    Science.gov (United States)

    ... chap 275. Raftery AT, Lim E, Ostor AJK. Joint disorders. In: Raftery AT, Lim E, Ostor AJK, eds. ... A.M. Editorial team. Related MedlinePlus Health Topics Joint Disorders Browse the Encyclopedia A.D.A.M., Inc. ...

  8. Review Article Therapeutic Potential of Statins in Age-related ...

    African Journals Online (AJOL)

    2011-08-09

    Aug 9, 2011 ... would bias any association towards the null. In 2005, Smeeth and others .... compared with patients in the lowest proportion of days covered (PDC) .... Guymer RH, Chiu AW, Lim L, Lim LL, Baird PN and. Varsamidis M (2005).

  9. THE EXISTENCE OF POSITIVE SOLUTIONS FOR A CLASS OF HAMMERSTEIN NONLINEAR INTEGRAL EQUATIONS%Hammerstein型非线性积分方程正解的存在性

    Institute of Scientific and Technical Information of China (English)

    张克梅; 孙经先

    2002-01-01

    lim u→0 inf{f(x,u)}/u≠lim u→0sup{f(x,u)}/u及lim u→+∞inf{f(x,u)}/u≠lim u→+∞sup{f(x,u)}/u的条件下,给出了Hammerstein型非线性积分方程:ψ(x)=∫ck(x,y)f(y,ψ(y))dy的一个正解的存在性定理.

  10. Clinical characteristics of patients with benign nonlesional temporal lobe epilepsy

    Directory of Open Access Journals (Sweden)

    Kim J

    2016-07-01

    Full Text Available Jiyeon Kim,1 Seong Hoon Kim,2 Sung Chul Lim,2 Woojun Kim,2 Young-Min Shon3 1Department of Neurology, Korea University Ansan Hospital, College of Medicine, Korea University, Ansan, 2Department of Neurology, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seocho-gu, 3Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Purpose: To evaluate the evolution of nonlesional temporal lobe epilepsy (TLE-NL in patients treated exclusively with antiepileptic drugs and to elucidate clinical phenotypes related to the prognosis of these patients.Methods: Clinical, radiological, and electroencephalographic (EEG findings in 84 patients with TLE-NL were reviewed. A good response group (GRG and a poor response group (PRG were defined if the duration of their seizure-free period was >1 year, or <1 year, respectively.Results: There were 46 (54.8% patients in the GRG and 38 (45.2% patients in the PRG. The number of antiepileptic drugs administered was significantly lower in the GRG than that in the PRG (1.3±0.8 vs 2.8±1.0, respectively; P<0.05. The GRG had a significantly older age of onset than the PRG and a lower occurrence of initial precipitating events, such as febrile seizures, central nervous system infection, and head trauma (P<0.05. The prevalence of EEG abnormality, presence of aura, generalized seizures, and automatism was less frequently observed in the GRG (P<0.05. Multivariate analysis showed that the presence of automatism and initial precipitating events were significantly associated with a poor prognosis (P<0.05.Conclusion: In contrast to the commonly assumed intractability of TLE, we found that more than 54% of patients with TLE-NL achieved a long seizure-free period. Older age at onset of TLE-NL was associated with a better prognosis. However, the presence of automatism and initial precipitating events were related to a poor prognosis. Future prospective

  11. Exposure–outcome analysis in depressed patients treated with paroxetine using population pharmacokinetics

    Directory of Open Access Journals (Sweden)

    Kim JR

    2015-09-01

    Full Text Available Jung-Ryul Kim,1 Hye In Woo,2 Mi-Ryung Chun,3 Shinn-Won Lim,4 Hae Deun Kim,5 Han Sung Na,5 Myeon Woo Chung,5 Woojae Myung,6 Soo-Youn Lee,1,3 Doh Kwan Kim6 1Department of Clinical Pharmacology and Therapeutics, Samsung Medical Center, Seoul, 2Department of Laboratory Medicine, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, 3Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 4Samsung Advanced Institute for Health Sciences and Technology (SAIHST, Sungkyunkwan University, Seoul, 5Clinical Research Division, Toxicological Evaluation and Research Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Chungcheongbuk-do, 6Department of Psychiatry, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea Purpose: This study investigated population pharmacokinetics of paroxetine, and then performed an integrated analysis of exposure and clinical outcome using population pharmacokinetic parameter estimates in depressed patients treated with paroxetine. Patients and methods: A total of 271 therapeutic drug monitoring (TDM data were retrospectively collected from 127 psychiatric outpatients. A population nonlinear mixed-effects modeling approach was used to describe serum concentrations of paroxetine. For 83 patients with major depressive disorder, the treatment response rate and the incidence of adverse drug reaction (ADR were characterized by logistic regression using daily dose or area under the concentration–time curve (AUC estimated from the final model as a potential exposure predictor. Results: One compartment model was developed. The apparent clearance of paroxetine was affected by age as well as daily dose administered at steady-state. Overall treatment response rate was 72%, and the incidence of ADR was 30%. The logistic regression showed that exposure

  12. Systematic Review and Meta-Analysis of TST Conversion Risk in Deployed Military and Long-Term Civilian Travelers

    Science.gov (United States)

    2010-01-01

    TB infec- tion risk in travelers and deployed military personnel. The three databases of PubMed Medline, Current Contents Connect, and EMBASE were...34Travel"[Majr], EMBASE -’tuberculosis’/mj and ’travel’/mj and [english]/lim and [humans]/lim and [ embase ]/lim, Cur- rent Contents Connect

  13. 国际交流(英文)

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Gu Xiulian Meets C.B.Sung,Wife顾秀莲会见沈坚白夫妇On December 20,2006,Gu Xiulian (middle),Vice-Chairperson of the Standing Committee of the National People's Congress and President of the All-China Women's Federation (ACWF),met C.B.Sung (second from left),Chairman of the Board of The 1990 Institute,based in the United States,and his wife,Beulah K.Sung (second from right),at the ACWF.

  14. Experiment list: SRX135563 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available || DIFFERENTIATION_STAGE=Induced pluripotent stem cell || DIFFERENTIATION_METHOD=As described by Yu, J. et a...sample_term_id=NTR_0001173 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bw/SRX135563.bw http://d...barchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/SRX135563.05.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed10/SRX135563.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed20/SRX135563.20.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bb05/SRX

  15. Experiment list: SRX056714 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ENTIATION_STAGE=Induced pluripotent stem cell || DIFFERENTIATION_METHOD=As described by Yu, J. et al. Human ... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bw/SRX056714.bw http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed05/SRX056714.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10.../SRX056714.10.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed20/...SRX056714.20.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bb05/SRX056714.05.bb http://dbarchive.biosciencedbc.jp/kyu

  16. Experiment list: SRX056704 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ENTIATION_STAGE=Induced pluripotent stem cell || DIFFERENTIATION_METHOD=As described by Yu, J. et al. Human ... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bw/SRX056704.bw http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed05/SRX056704.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10.../SRX056704.10.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed20/...SRX056704.20.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bb05/SRX056704.05.bb http://dbarchive.biosciencedbc.jp/kyu

  17. Experiment list: SRX081222 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available TIATION_STAGE=Induced pluripotent stem cell || DIFFERENTIATION_METHOD=As described by Yu, J. et al. Human in... http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bw/SRX081222.bw http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed05/SRX081222.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10.../SRX081222.10.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed20/...SRX081222.20.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bb05/SRX081222.05.bb http://dbarchive.biosciencedbc.jp/kyu

  18. 关于不定型1∞极限的几种求法

    Institute of Scientific and Technical Information of China (English)

    潘晓鸣

    2008-01-01

    @@ 若极限嗽lim x→x0(x→∞)f(x)g1-型,lim x→x0(x→∞)f(x)=1,lim x→x0(x→∞)g(x)=∞,则极限的四则运算法则对它无效.现把求这种极限常见的几种方法列举如下. 1.用重要极限lim x→∞(1+1/x)x=e求极限 例1 求极限lim x→∞(x2+a2/x2-a2)x2.

  19. In-Situ Scanning Electron Microscopy Comparison of Microstructure and Deformation Between WE43-F and WE43-T5 Magnesium Alloys

    Science.gov (United States)

    2012-05-01

    Kyu Cho Weapons and Materials Research Directorate, ARL Bruce Davis and Richard De Lorme Magnesium Elektron North America A...345–348, 2011. * Magnesium Elektron North America, 1001 College St., Madison, IL 62060 14. ABSTRACT In-situ tensile testing in the scanning...Materials and Manufacturing Science Division, Aberdeen Proving Ground, MD 21005 USA 2. Magnesium Elektron North America, 1001 College St. Madison, IL

  20. Fine Scale Spatiotemporal Clustering of Dengue Virus Transmission in Children and Aedes aegypti in Rural Thai Villages

    Science.gov (United States)

    2012-07-01

    Fine Scale Spatiotemporal Clustering of Dengue Virus Transmission in Children and Aedes aegypti in Rural Thai Villages In-Kyu Yoon1*, Arthur Getis2...between infected humans and Aedes aegypti in Thai villages. Methodology/Principal Findings: Geographic cluster investigations of 100-meter radius were...in rural Thailand. Child contacts and Ae. aegypti from cluster houses were assessed for DENV infection. Spatiotemporal, demographic, and entomological

  1. Experiment list: SRX386361 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ve.biosciencedbc.jp/kyushu-u/sacCer3/eachData/bed05/SRX386361.05.bed http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/eachData/bed...10/SRX386361.10.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/sacCer3/eachData/bed20/SRX386361.20.bed http://dbarchive.biosciencedbc.jp/kyushu-u/sacCer3/eachData/bb

  2. China’s Foreign Policy Toward North Korea: The Nuclear Issue

    Science.gov (United States)

    2012-12-01

    pressure on North Korea by not opposing the adoption of a Security Council resolution calling for denuclearization of North Korea. Heung-kyu Kim also...South Korea. The two Koreas also signed the Joint Declaration on Denuclearization of the Korean peninsula, which declared they would not test...Declaration on The Denuclearization of The Korean Peninsula, http://www.fas.org/news/dprk/1992/920219-D4129.htm. 13 Confronted with this brinkmanship

  3. Eine lohnende Investition mit ungewissem Ausgang

    OpenAIRE

    Seelos, Christian; Mair, Johanna

    2016-01-01

    Innovation in sozialen Organisationen bedeutet vor allem eines nicht: die Garantie auf eine schnelle Lösung von Problemen. Vielmehr ist Innovation ein Prozess, der das Lernpotenzial des Unternehmens steigert und damit auch den gewollten Mehrwert generiert.

  4. Music training and rate of presentation as mediators of text and song recall

    National Research Council Canada - National Science Library

    Kilgour, Andrea R; Jakobson, Lorna S; Cuddy, Lola L

    2000-01-01

    The present research addresses whether music training acts as a mediator of the recall of spoken and sung lyrics and whether presentation rate is the essential variable, rather than the inclusion of melody...

  5. Time-and Space-Resolved Spectroscopic Investigation on pi-Conjugated Nanostructures

    Science.gov (United States)

    2014-07-30

    Bonding Interactions of meso-Imidazolyl Groups Hirotaka Mori, Young Mo Sung, Byung Sun Lee, Dongho Kim and Atsuhiro Osuka Angew . Chem. Int. Ed...Chi, Jun Ding, Juan Casado, Dongho Kim and Jishan Wu Angew . Chem. Int. Ed., 52(33), 8561-8565. August 12, 2013 13. S2 Fluorescence Dynamics of...meso-Aryl-substituted Subporphyrins Jooyoung Sung, Pyosang Kim, Shun Saga, Shin-ya Hayashi, Atsuhiro Osuka, and Dongho Kim Angew . Chem. Int. Ed., 52

  6. Robust Speech Processing & Recognition: Speaker ID, Language ID, Speech Recognition/Keyword Spotting, Diarization/Co-Channel/Environmental Characterization, Speaker State Assessment

    Science.gov (United States)

    2015-10-01

    integrated spectral and prosody based LID solutions. For co-channel and diarization, new algorithms based on gammatone subband frequency modulation was...17 9 Vowel classification accuracy or spoken & sung vowels using k-NN classifier ..….……. 19 10 Vowel classification accuracy for spoken & sung...27 18 TO-Combo-SAD decisions and scream detection using CompSeg ………………………. 29 19 Distribution of spectral center of gravity (SCG) in

  7. Species of Cordyceps-like Fungi from Guangxi, China%广西的虫草种类

    Institute of Scientific and Technical Information of China (English)

    宋斌; 吴兴亮; 李泰辉

    2011-01-01

    本文报道了广西虫草类真菌2科3属23种,其中珊瑚虫草Cordyceps martialis Speg.、红座虫草Cordyceps roseostromataKobayasi & Shimizu和蚁窝线虫草Ophiocordyceps formicarum(Kobayasi)G.H.Sung,J.M.Sung,Hywel-Jones & Spatafora是广西新记录种.

  8. Happy Handwashing Song

    Centers for Disease Control (CDC) Podcasts

    2010-02-25

    This song (sung to the tune of Happy Birthday) encourages kids to wash their hands with soap and water to keep germs away. The song is sung twice through, the recommended length of time to wash hands. Sing along!  Created: 2/25/2010 by National Center for Preparedness, Detection, and Control of Infectious Diseases (NCPDCID), Division of Global Migration and Quarantine (DGMQ).   Date Released: 2/25/2010.

  9. Formation of Acetic Acid by Aqueous-Phase Oxidation of Ethanol with Air in the Presence of a Heterogeneous Gold Catalyst

    DEFF Research Database (Denmark)

    Christensen, Claus H.; Jørgensen, B.; Hansen, Jeppe Rass

    2006-01-01

    Die selektive Oxidation von Ethanol zu Essigsäure gelingt in wässriger Lösung mit dem Oxidans Luft an einem Gold-Heterogenkatalysator (siehe Bild). Bei 423 K und einem O2-Druck von 0.6 MPa verläuft diese Reaktion glatt in saurer wässriger Lösung in Ausbeuten um 90 %. CO2 ist das einzige Nebenprod...

  10. Mongghul Duraasi Dau

    OpenAIRE

    Ha, Mingzong

    2009-01-01

    Two Mongghul drinking songs were recorded. The first song is sung in Tibetan, while the second is in local Chinese. Most Mongghul songs are in Mongghul, however, a few are in the local Tibetan and Chinese languages. Song one: This Mongghul song sung in Tibetan shows close historical contact between the Mongghul and Tibetan. Today, as Chinese pop music permeates the daily lives of Mongghul people and as the mass media diverts attention from folksongs, young Monggh...

  11. Happy Handwashing Song

    Centers for Disease Control (CDC) Podcasts

    2010-02-25

    This song (sung to the tune of Happy Birthday) encourages kids to wash their hands with soap and water to keep germs away. The song is sung twice through, the recommended length of time to wash hands. Sing along!  Created: 2/25/2010 by National Center for Preparedness, Detection, and Control of Infectious Diseases (NCPDCID), Division of Global Migration and Quarantine (DGMQ).   Date Released: 2/25/2010.

  12. Comparison of hydration and nutritional status between young and elderly hemodialysis patients through bioimpedance analysis

    Directory of Open Access Journals (Sweden)

    Lee JE

    2015-08-01

    Full Text Available Jung Eun Lee,1,2 In Young Jo,3 Song Mi Lee,3 Woo Jeong Kim,3 Hoon Young Choi,2,4 Sung Kyu Ha,4 Hyung Jong Kim,5 Hyeong Cheon Park2,4 1Department of Internal Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, 2Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, 3Department of Nutrition Services, Gangnam Severance Hospital, 4Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, 5Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea Background: The number of elderly people on dialysis is increasing rapidly. Fluid overload and malnutrition status are serious problems in elderly dialysis patients. We aimed to compare the hydration and nutritional status through bioimpedance analysis (BIA between young and elderly hemodialysis (HD patients and to analyze risk factors related to fluid overload and malnutrition status in these patients.Method: We conducted a cross-sectional study, in which 82 HD (males 42, mean age 58.7±12.9 years patients were enrolled. We collected different types of data: laboratory data, such as serum creatinine, albumin, total iron-binding capacity, hemoglobin, total cholesterol; anthropometric data, such as hand grip strength (HGS; BIA data, such as intracellular water, skeletal muscle mass, body cell mass, bone mineral content, phase angle (PhA, extra cellular water (ECW/total body water (TBW ratio; and malnutrition-inflammation score (MIS, which is a traditional nutritional parameter for dialysis patients. All patients were stratified into two groups according to their age: young (<65 years [n=54] and elderly (≥65 years [n=28].Results: Total iron-binding capacity and HGS were significantly lower in elderly HD patients than in young HD patients (198.9±35.6 vs 221.4±52.1 mcg/dL; and 22.4±10.3 vs 36.4±23.2 kg, respectively (P<0.05. Also, intracellular water and Ph

  13. Baobab Laboratory Information Management System: Development of an Open-Source Laboratory Information Management System for Biobanking.

    Science.gov (United States)

    Bendou, Hocine; Sizani, Lunga; Reid, Tim; Swanepoel, Carmen; Ademuyiwa, Toluwaleke; Merino-Martinez, Roxana; Meuller, Heimo; Abayomi, Akin; Christoffels, Alan

    2017-04-01

    A laboratory information management system (LIMS) is central to the informatics infrastructure that underlies biobanking activities. To date, a wide range of commercial and open-source LIMSs are available and the decision to opt for one LIMS over another is often influenced by the needs of the biobank clients and researchers, as well as available financial resources. The Baobab LIMS was developed by customizing the Bika LIMS software ( www.bikalims.org ) to meet the requirements of biobanking best practices. The need to implement biobank standard operation procedures as well as stimulate the use of standards for biobank data representation motivated the implementation of Baobab LIMS, an open-source LIMS for Biobanking. Baobab LIMS comprises modules for biospecimen kit assembly, shipping of biospecimen kits, storage management, analysis requests, reporting, and invoicing. The Baobab LIMS is based on the Plone web-content management framework. All the system requirements for Plone are applicable to Baobab LIMS, including the need for a server with at least 8 GB RAM and 120 GB hard disk space. Baobab LIMS is a server-client-based system, whereby the end user is able to access the system securely through the internet on a standard web browser, thereby eliminating the need for standalone installations on all machines.

  14. Low-index-metamaterial for gain enhancement of planar terahertz antenna

    Directory of Open Access Journals (Sweden)

    Qing-Le Zhang

    2014-03-01

    Full Text Available We theoretically present a high gain planar antenna at terahertz (THz frequencies by combing a conventional log-periodic antenna (LPA with a low-index-metamaterial (LIM, |n| < 1. The LIM is realized by properly designing a fishnet metamaterial using full-wave finite-element simulation. Owing to the impedance matching, the LIM can be placed seamlessly on the substrate of the LPA without noticeable reflection. The effectiveness of using LIM for antenna gain enhancement is confirmed by comparing the antenna performance with and without LIM, where significantly improved half-power beam-width (3-dB beam-width and more than 4 dB gain enhancement are seen within a certain frequency range. The presented LIM-enhanced planar THz antenna is compact, flat, low profile, and high gain, which has extensive applications in THz systems, including communications, radar, and spectroscopy.

  15. Low-index-metamaterial for gain enhancement of planar terahertz antenna

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Qing-Le; Si, Li-Ming, E-mail: lms@bit.edu.cn; Lv, Xin [Beijing Key Laboratory of Millimeter Wave and Terahertz Technology, Department of Electronic Engineering, School of Information and Electronics, Beijing Institute of Technology, Beijing 100081 (China); Huang, Yongjun [Key Laboratory of Broadband Optical Fiber Transmission and Communication Networks, School of Communication and Information Engineering, University of Electronic Science and Technology of China, Chengdu, 611731 (China); Zhu, Weiren, E-mail: weiren.zhu@monash.edu [Advanced Computing and Simulation Laboratory (A chi L), Department of Electrical and Computer Systems Engineering, Monash University, Clayton, VIC 3800 (Australia)

    2014-03-15

    We theoretically present a high gain planar antenna at terahertz (THz) frequencies by combing a conventional log-periodic antenna (LPA) with a low-index-metamaterial (LIM, |n| < 1). The LIM is realized by properly designing a fishnet metamaterial using full-wave finite-element simulation. Owing to the impedance matching, the LIM can be placed seamlessly on the substrate of the LPA without noticeable reflection. The effectiveness of using LIM for antenna gain enhancement is confirmed by comparing the antenna performance with and without LIM, where significantly improved half-power beam-width (3-dB beam-width) and more than 4 dB gain enhancement are seen within a certain frequency range. The presented LIM-enhanced planar THz antenna is compact, flat, low profile, and high gain, which has extensive applications in THz systems, including communications, radar, and spectroscopy.

  16. Analysis and measure of novel stereo-garage driven by linear induction motor

    Directory of Open Access Journals (Sweden)

    Lu Qinfen

    2015-12-01

    Full Text Available The car access time is a key parameter, especially in a huge stereo-garage, where this one should be decreased as much as possible. This paper proposes a novel stereo-garage. Adopting the linear induction motors (LIMs, the system has a simple structure and rapid response capability. In the stereo-garage, several LIMs are installed below the crossbeam on a lifting platform, and several LIMs are fixed on the top of a moving frame. During the operation of LIMs, the moving frame moves forward and backward to reach the required parking place, whereas the crossbeam moves horizontally in order to take or store the vehicle rapidly. All these LIMs are the same and should be designed at a low frequency. The influences of key structure parameters and dynamic performances are investigated, based on FEM. The predicted results are validated by a prototype. Finally, the designed LIMs are successfully applied in two 8-layer stereo-garages.

  17. Rail Brake System Using a Linear Induction Motor for Dynamic Braking

    Science.gov (United States)

    Sakamoto, Yasuaki; Kashiwagi, Takayuki; Tanaka, Minoru; Hasegawa, Hitoshi; Sasakawa, Takashi; Fujii, Nobuo

    One type of braking system for railway vehicles is the eddy current brake. Because this type of brake has the problem of rail heating, it has not been used for practical applications in Japan. Therefore, we proposed the use of a linear induction motor (LIM) for dynamic braking in eddy current brake systems. The LIM reduces rail heating and uses an inverter for self excitation. In this paper, we estimated the performance of an LIM from experimental results of a fundamental test machine and confirmed that the LIM generates an approximately constant braking force under constant current excitation. At relatively low frequencies, this braking force remains unaffected by frequency changes. The reduction ratio of rail heating is also approximately proportional to the frequency. We also confirmed that dynamic braking resulting in no electrical output can be used for drive control of the LIM. These characteristics are convenient for the realization of the LIM rail brake system.

  18. Does music training facilitate the mnemonic effect of song? An exploration of musicians and nonmusicians with and without Alzheimer's dementia.

    Science.gov (United States)

    Baird, Amee; Samson, Séverine; Miller, Laurie; Chalmers, Kerry

    2017-02-01

    The efficacy of using sung words as a mnemonic device for verbal memory has been documented in persons with probable Alzheimer's dementia (AD), but it is not yet known whether this effect is related to music training. Given that music training can enhance cognitive functioning, we explored the effects of music training and modality (sung vs. spoken) on verbal memory in persons with and without AD. We used a mixed factorial design to compare learning (5 trials), delayed recall (30-min and, 24-hour), and recognition of sung versus spoken information in 22 healthy elderly (15 musicians), and 11 people with AD (5 musicians). Musicians with AD showed better total learning (over 5 trials) of sung information than nonmusicians with AD. There were no significant differences in delayed recall and recognition accuracy (of either modality) between musicians with and without AD, suggesting that music training may facilitate memory function in AD. Analysis of individual performances showed that two of the five musicians with AD were able to recall some information on delayed recall, whereas the nonmusicians with AD recalled no information on delay. The only significant finding in regard to modality (sung vs. spoken) was that total learning was significantly worse for sung than spoken information for nonmusicians with AD. This may be due to the need to recode information presented in song into spoken recall, which may be more cognitively demanding for this group. This is the first study to demonstrate that music training modulates memory of sung and spoken information in AD. The mechanism underlying these results is unclear, but may be due to music training, higher cognitive abilities, or both. Our findings highlight the need for further research into the potentially protective effect of music training on cognitive abilities in our aging population.

  19. Measurement of Drive Characteristics of Linear Induction Motor with Experimental Equipment Implemented Disc-shaped Secondary Side

    Science.gov (United States)

    Morizane, Toshimitsu; Kimura, Noriyuki

    The ordinary experimental equipment has a long stroke or a large cylindrical rotating secondary side in order to measure the drive characteristics of Linear Induction Motor (LIM). In this paper, we propose the measurement method of the drive characteristics of LIM with the experimental equipment implemented a disc-shaped secondary side. This method makes the experimental equipment smaller in size. It has been shown that the drive characteristics of LIM can be successfully measured.

  20. Analysis of chirality by femtosecond laser ionization mass spectrometry.

    Science.gov (United States)

    Horsch, Philipp; Urbasch, Gunter; Weitzel, Karl-Michael

    2012-09-01

    Recent progress in the field of chirality analysis employing laser ionization mass spectrometry is reviewed. Emphasis is given to femtosecond (fs) laser ionization work from the author's group. We begin by reviewing fundamental aspects of determining circular dichroism (CD) in fs-laser ionization mass spectrometry (fs-LIMS) discussing an example from the literature (resonant fs-LIMS of 3-methylcyclopentanone). Second, we present new data indicating CD in non-resonant fs-LIMS of propylene oxide.

  1. Single neuron network PI control of high reliability linear induction motor for Maglev

    Institute of Scientific and Technical Information of China (English)

    FANG You-tong; FAN Cheng-zhi

    2007-01-01

    The paper deals with a new model of linear induction motor (LIM) to improve the reliability of the system. Based on the normal equation circuit of LIM considering the dynamic end effect, an equivalent circuit model with compensation of large end effect is constructed when the end effect force at synchronism is of braking character. The equivalent circuit model is used for secondary-flux oriented control of LIM. Single neuron network PI unit for LIM servo-drive is also discussed. The effectiveness of mathematical model for drive control is verified by simulations.

  2. 交换算子组相似极限的联合谱半径%The Joint Spectral Radius for Iimits of Similarities of N-commuting Operators

    Institute of Scientific and Technical Information of China (English)

    翟发辉; 王泽华; 田保光

    2007-01-01

    设{Xn:n=1,2,…}是定义在Hilbert空间H上有界可逆算子序列,T=(T1,T2,…Tn)是有界交换算子组,XmTX-1m=(XmT1X-1m,XmT2X-1m,…,XmTnX-1m,…,XmnX-1m),证明了如果Ξ={T=(T1,T2,…,Tn)∈L(H)ncom:lim(m→∞)XmTX-1m存在}是闭集,T∈Ξ且lim(m→∞)XmTX-1m=(lim(m→∞)XmT1X-1m,lim(m→∞)XmT2X-1m,…,lim(m→∞)XmTnX-1m)∈L(H)ncom,则T与lim(m→∞)XmTX-1m的联合谱半径满足rsp(T)≤rsp(lim(m→∞)XmTX-1m)≤lim(s→∞)(∑(α∈Zn+,|α|=s)(s!/α!)‖Tα11…Tαnn‖2)1/(2s),这里|α|=∑ni=1αi,α!=α1!…αn!,同时也给出了算子组T与lim(m→∞)XmTX-1m的共同不变子空间.

  3. Simulating the mass balance and salinity of Arctic and Antarctic sea ice. 1. Model description and validation

    OpenAIRE

    Vancoppenolle, M.; Fichefet, T.; Goosse, H.; Bouillon, S; Madec, G.; Maqueda, M.A.M.

    2009-01-01

    This paper is the first part of a twofold contribution dedicated to the new version of the Louvain-la-Neuve sea ice model LIM3. In this part, LIM3 is described and its results arc, compared with observations. LIM3 is a C-grid dynamic-thermodynamic model, including the representation of the subgrid-scale distributions of ice thickness, enthalpy, salinity and age. Brine entrapment and drainage as well as brine impact on ice thermodynamics are explicitly included. LIM3 is embedded into the ocean...

  4. For 5-Month-Old Infants, Melodies Are Social.

    Science.gov (United States)

    Mehr, Samuel A; Song, Lee Ann; Spelke, Elizabeth S

    2016-04-01

    For 1 to 2 weeks, 5-month-old infants listened at home to one of two novel songs with identical lyrics and rhythms, but different melodies; the song was sung by a parent, emanated from a toy, or was sung live by a friendly but unfamiliar adult first in person and subsequently via interactive video. We then tested the infants' selective attention to two novel individuals after one sang the familiar song and the other sang the unfamiliar song. Infants who had experienced a parent singing looked longer at the new person who had sung the familiar melody than at the new person who had sung the unfamiliar melody, and the amount of song exposure at home predicted the size of that preference. Neither effect was observed, however, among infants who had heard the song emanating from a toy or being sung by a socially unrelated person, despite these infants' remarkable memory for the familiar melody, tested an average of more than 8 months later. These findings suggest that melodies produced live and experienced at home by known social partners carry social meaning for infants. © The Author(s) 2016.

  5. Organization of research team for nano-associated safety assessment in effort to study nanotoxicology of zinc oxide and silica nanoparticles

    Directory of Open Access Journals (Sweden)

    Kim YR

    2014-12-01

    Full Text Available Yu-Ri Kim,1,* Sung Ha Park,2,* Jong-Kwon Lee,3 Jayoung Jeong,3 Ja Hei Kim,4 Eun-Ho Meang,5 Tae Hyun Yoon,6 Seok Tae Lim,7 Jae-Min Oh,8 Seong Soo A An,9 Meyoung-Kon Kim1 1Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea; 2Department of Biochemistry, University of Bath, Bath, UK; 3Toxicological Research Division, National Institute of Food and Drug Safety Evaluation, Chungchungbuk-do, 4Consumers Korea, Chongro-ku, 5General toxicology team, Korea Testing and Research Institute, 6Laboratory of Nanoscale Characterization and Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, 7Department of Nuclear Medicine, Chonbuk National University Medical School, Jeonju, Jellabuk-Do, 8Department of Chemistry and Medical Chemistry, College of Science and Technology, Yonsei University, Gangwon-do, 9Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, South Korea *Authors contributed equally to this work Abstract: Currently, products made with nanomaterials are used widely, especially in biology, biotechnologies, and medical areas. However, limited investigations on potential toxicities of nanomaterials are available. Hence, diverse and systemic toxicological data with new methods for nanomaterials are needed. In order to investigate the nanotoxicology of nanoparticles (NPs, the Research Team for Nano-Associated Safety Assessment (RT-NASA was organized in three parts and launched. Each part focused on different contents of research directions: investigators in part I were responsible for the efficient management and international cooperation on nano-safety studies; investigators in part II performed the toxicity evaluations on target organs such as assessment of genotoxicity, immunotoxicity, or skin penetration; and investigators in part III evaluated the toxicokinetics of NPs with newly developed

  6. Analysis list: FOS [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available FOS Blood,Breast,Digestive tract,Uterus + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/FOS....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/FOS.5.tsv http://dbarchi...ve.biosciencedbc.jp/kyushu-u/hg19/target/FOS.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/FOS....Blood.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/FOS.Breast.tsv,ht...tp://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/FOS.Digestive_tract.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/FOS

  7. Analysis list: unc-62 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available unc-62 Adult,Embryo,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/unc-62.1.tsv http:...//dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/unc-62.5.tsv http://dbarchive.bios...ciencedbc.jp/kyushu-u/ce10/target/unc-62.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/unc-62.Adult.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/unc-62.Embryo.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/unc-62.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyu

  8. Experiment list: SRX129092 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available truction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIE...e_name=Multiple myeloma || chip antibody=H3K4me3 || cell type=Plasma cell || duration=24hours || drug=DMSO http://dbarchive.bioscie...ncedbc.jp/kyushu-u/hg19/eachData/bw/SRX129092.bw http://dbarchive.bioscie...ncedbc.jp/kyushu-u/hg19/eachData/bed05/SRX129092.05.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed10/SRX129092.10.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed20/SR

  9. Experiment list: SRX129091 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available truction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIE...e_name=Multiple myeloma || chip antibody=H3K27Ac || cell type=Plasma cell || duration=24hours || drug=DMSO http://dbarchive.bioscie...ncedbc.jp/kyushu-u/hg19/eachData/bw/SRX129091.bw http://dbarchive.bioscie...ncedbc.jp/kyushu-u/hg19/eachData/bed05/SRX129091.05.bed http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/eachData/bed10/SRX129091.10.bed http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed20/SR

  10. History of the Great Patriotic War of the Soviet Union, 1941-1945. Volume 6. Results of the Great Patriotic War,

    Science.gov (United States)

    1982-10-06

    with the general/common/total enemyŖ. FOOTNOTE 1. Robert Sherwood. Roosevelt and Hopkins by the eyes of eyewitness, Vol. 2, p. 613. ENDFOOTNOTE. With...34, created with the aid of the military activities? Specialist in questions of the military doctrine of the USA Robert Tucker, the former consultant of...Central Committee of the CPA and the news- paper "Kyu Kuok " ("Rescue of the Fatherland") which was the publi- cation of the Viet Mingh. Not a small role

  11. Experiment list: ERX210215 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ERX210215 hg19 No description NA Breast Breast cancer cells NA 28866329,96.7,4.9,11...cer || cell type=epithelial http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bw/ERX21021...5.bw http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed05/ERX210215.05.bed http://d...barchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bed10/ERX210215.10.bed http://dbarchive.biosciencedbc.jp/ky...ushu-u/hg19/eachData/bed20/ERX210215.20.bed http://dbarchive.biosciencedbc.jp/kyu

  12. Analysis list: Srf [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Srf Blood,Embryonic fibroblast,Muscle + mm9 http://dbarchive.biosciencedbc.jp/kyush...u-u/mm9/target/Srf.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Srf.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/target/Srf.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Srf.Blood....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Srf.Embryonic_fibroblast....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Srf.Muscle.tsv http://dbarchive.biosciencedbc.jp/kyu

  13. Analysis list: CBP [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available CBP Adult,Cell line,Embryo,Larvae,Pupae + dm3 http://dbarchive.biosciencedbc.jp/kyu...shu-u/dm3/target/CBP.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/target/CBP.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/dm3/target/CBP.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/colo/CBP.Adul...t.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/dm3/colo/CBP.Cell_line.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/dm3/colo/CBP.Embryo.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/dm3

  14. Analysis list: ZFX [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ZFX Blood,Neural + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ZFX.1.tsv http://dbarchive....biosciencedbc.jp/kyushu-u/hg19/target/ZFX.5.tsv http://dbarchive.biosciencedbc.jp/kyu...shu-u/hg19/target/ZFX.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/ZFX.Blood.tsv,http://dbarchive....biosciencedbc.jp/kyushu-u/hg19/colo/ZFX.Neural.tsv http://dbarchive.bioscien...cedbc.jp/kyushu-u/hg19/colo/Blood.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Neural.gml ...

  15. Analysis list: nfya-1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available nfya-1 Adult,Embryo,Larvae + ce10 http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/nf...ya-1.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/target/nfya-1.5.tsv http://dbarchive.bios...ciencedbc.jp/kyushu-u/ce10/target/nfya-1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/nfya-1....Adult.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/nfya-1.Embryo.tsv,...http://dbarchive.biosciencedbc.jp/kyushu-u/ce10/colo/nfya-1.Larvae.tsv http://dbarchive.biosciencedbc.jp/kyu

  16. Analysis list: POU5F1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available POU5F1 Epidermis,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyu...archive.biosciencedbc.jp/kyushu-u/hg19/target/POU5F1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/POU5F1.Epidermis...U5F1.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Epidermis.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Pluripotent_stem_cell.gml ...

  17. Aikido experience and its relation to mindfulness: a two-part study.

    Science.gov (United States)

    Lothes, John; Hakan, Robert; Kassab, Karin

    2013-02-01

    The martial art Aikido may be useful in the development of mindfulness. In this study, the authors examined the potential association of training in Aikido may have on mindfulness. The sample of Study 1 comprised 159 participants completing two empirically validated scales of mindfulness and awareness, the Kentucky Inventory of Mindfulness Skills and the Mindfulness Attention Awareness Scale. Study 2 was a longitudinal study of 20 Aikido students from beginning through 5th Kyu (approximately 9 months of training). The results from both studies show significant increases in mindfulness scores with increased Aikido training.

  18. 掺稀土的LiM0.02Mn1.98O4锂离子电池正极材料%Rare Earth Elements-Doped Spinel LiM0.02Mn1.98O4 as the Cathodic Materials for Lithium Ion Batteries

    Institute of Scientific and Technical Information of China (English)

    万传云; 努丽燕娜; 江志裕

    2002-01-01

    @@ 自1991年Ohzuku[1] 、 Tarascon[2]等成功地将LiMn2O4用于锂离子电池正极以来, 人们对尖晶石LiMn2O4的电化学性质进行了广泛的研究[3]. 尖晶石LiMn2O4的一个缺点是充放电过程中, 特别在较高温度(如50 ℃)下, 其容量下降明显. Zhou等[4]详细研究了该过程, 发现造成容量下降的主要原因是充电状况下正极LiMn2O4的溶解, 由于Jahn-Taller效应生成不稳定的两相结构以及电解液的分解等. 为了提高LiMn2O4的充放电循环稳定性, 人们除了优化合成条件和溶液组分外, 主要采用添加少量掺杂元素(M), 部分替代LiMn2O4中的Mn, 制得LiMxMn2-xO4, 以抑制溶解和Jahn-Taller效应引起的结构变化.

  19. Influência da luz e da temperatura sobre a oxidação do óleo essencial de capim-limão (Cymbopogon citratus (D.C. Stapf Influence of light and temperature on the oxidation of the essential oil of lemongrass (Cymbopogon Citratus (D.C. Stapf

    Directory of Open Access Journals (Sweden)

    Luiz Gustavo de L. Guimarães

    2008-01-01

    Full Text Available The identification of the chemical compounds of the essential oil was performed with a gas chromatograph coupled to a mass spectrometer. The oil was left in the presence and absence of light and submitted to different temperatures to evaluate its stability. The yields of the major compounds were evaluated every fifteen days. Citral and myrcene, the major compounds of the essential oil, were degraded over time in both the presence and absence of light, but temperature only influenced the degradation of myrcene.

  20. Isolation, expression and construction of plant expression vector of PdLim1, a transcription factor involved in the lignin biosynthesis in Populua deltoides%美洲黑杨木质素生物合成转录因子PdLiml基因的克隆、表达及植物表达载体构建

    Institute of Scientific and Technical Information of China (English)

    王璐; 李百炼; 张金凤; 张德强

    2009-01-01

    在植物体内,Liml基因与木质素生物合成酶基因启动子区域富含AC的Pal盒结合,转录调控木质素的生物合成过程.该研究组合利用生物信息学和realtime PCR方法,首次从美洲黑杨形成层cDNA中分离出PdLimlcDNA全长,并进行了测序和序列分析.结果表明,克隆的美洲黑杨PdLimlcDNA片段总长为952 bp,基因内部含有完整的开放阅读框架,大小为594 bp,可编码长度为197个氨基酸残基的蛋白质,所推导的蛋白质氨基酸序列与拟南芥AtLiml和水稻OsLiml蛋白的同源性分别为82.1%和78.2%.组织特异性realtime PCR结果显示,PdLiml基因在场树根、茎、叶片和顶端分生组织中均有表达,但其表达模式却不同:PdLiml在成熟叶片和成熟木质部中表达丰度最高,在树皮、根部、韧皮部和形成层表达丰度较高,在未成熟木质部有少量表达,在顶端分生组织中表达丰度最低.在此基础上,以pBI121为表达载体,构建了在CaMV35S启动子驱动下,PdLiml基因的正义(pBI121-CaMV35S-sensePdLiml)和反义(pBI121-CaMV35S-antisense PdLiml)类型的双元植物表达载体.该研究为杨树PdLiml的基因工程改良木材纤维品质性状提供了重要的理论依据,具有潜在的应用价值.%In plants, the Liml gene regulates the lignin biosynthesis by binding the AC-rich motif, the Pal box, which is an important cis-acting element located in the promoter region of genes encoding enzymes in the lignin biosynthesis. A full-length cDNA clone encoding Liml was isolated from the cDNA prepared from the cambium zone of Populus deltoides by the approach of biological informatics and realtime PCR method. The cDNA was 952 bp in length with an open reading frame (ORF), which is capable of encoding a protein of 197 amino acids. The deduced protein sequence of the PdLiml shared 82.1% and 78.2% identity with those of Arabidopsis thaliana and Oryza sativa Liml,respectively. Realtime PCR results indicated that PdLiral was expressed in root, stem, leave and apical shoot meristems, but different in expression level. The PdLiml transcripts were the most abundant in mature leaf and xylem, with higher expression in bark, root, phloem and cambium, with some expression in the immature xylem, but a low-abundance was detected in apical shoot meristems. The plant binary vectors, in which the PdLiml cDNA was introduced in sense or antisense orientation to the CaMV35S promoter, I.e. pBI121-CaMV35S-sense PdLiml and pBI121-CaMV35S-antisense PdLiml, were constructed. The results, therefore, provide important foundation for the improvement of wood fiber property by the strategy of gene engineering of PdLiml and have potential application in tree breeding.

  1. Efecto de tres abonos orgánicos: lombricomposta, bocashi y tierra de corral en un cultivo intercalado de jitomate (Solanum lycopersicon) y pepino (Cucumis sativus) bajo invernadero en el ejido El Limón, Tepalcingo, Morelos, México

    OpenAIRE

    Hernández Tapia, Alejandro; Ramírez-Rivero,Libia Aidé; Corlay-Chee, Langen; Cruz Rodríguez, Juan Antonio

    2015-01-01

    Se evaluó una intercalación de jitomate y pepino bajo condiciones de invernadero, con un diseño de bloques completos al azar, con nueve tratamientos de abono en cuatro repeticiones (Bocashi, lombricomposta y tierra de corral con tres niveles de dosificación 0.5, 1.0 y 1.5 kg/m2).Se evaluó rendimiento y,en el caso del jitomate número de frutos dañados. Se empleó un análisis de varianza (ANOVA) y pruebas de comparación de medias (Tukey α=.05). En el caso del pepino, con el tratamiento lombri...

  2. Efeitos agudos dos extratos hidroalcoólicos do alho (Allium sativum L. e do capim-limão (Cymbopogon citratus (DC Stapf sobre a pressão arterial média de ratos anestesiados Acute effects of Allium sativum L. and Cympobongon citratus (DC Stapf hydroalcoholic extracts on arterial blood pressure of anesthetized rats

    Directory of Open Access Journals (Sweden)

    G. Singi

    2005-06-01

    Full Text Available Este trabalho objetivou verificar os efeitos da aplicação aguda endovenosa dos extratos hidroalcoólicos de Allium sativum e de Cymbopogon citratus sobre a pressão arterial de ratos. Foram usados Rattus novergicus albinus, n=7, anestesiados, traqueostomizados e canulados através da veia jugular e da artéria carótida. Foram injetadas doses de 1 mg dos extratos separadamente e em associação (1mg + 1mg, em volumes de 0,2mL. A pressão arterial média (PAM foi registrada com um sistema Biopac, modelo MP100. O Allium sativum diminuiu a PAM de 124±2 mmHg, no controle, para 108±2 mmHg aos 15s. Da mesma forma, o Cymbopogon citratus diminuiu a PAM de 122±2 mmHg, no controle, para 106± 2 mmHg aos 15s. A associação de ambos também diminuiu a PAM de 126±3 mmHg, no controle, para 113±3 mmHg aos 15s. Os efeitos das duas plantas foram iguais e não foram incrementados quando associadas.This work aimed to verify the effects of acute intravenous applications of Allium sativum and Cymbopogon citratus hydroalcoholic extracts on arterial blood pressure of anesthetized rats. Adult male rats (Rattus novergicus albinus, n=7, were used. The rats were anesthetized, tracheotomy and cannulation of both jugular and carotid were carried out. The injected doses were 1 mg separately as well as the association of both extracts, in volumes of 0,2 mL. The mean arterial blood pressure (MAP was recorded with a Biopac System, model MP100. The Allium sativum decreased the MAP only from 124±2 mmHg (control to 108±2 mmHg at 15s. The Cymbopogon citratus also decreased the MAP from 122±2 mmHg (control to 106±2 mmHg after 15s. The 1mg of Allium sativum + 1mg of Cymbopogon citratus also decreased the MAP from 126±3 mmHg (control to 113±3 mmHg after 15s. The effects of the two plants were the same and were not increased when in association.

  3. Tokat’ın 17. Yüzyıl Âlim Şairlerinden İshak Bin Hasan Tokatî ve Nazmu’l-Ulum Adlı Mesnevîsi Poetry in Rhymed Couplets of Tokatlı İshâk Efendi Nazmu'l-Ulûm

    Directory of Open Access Journals (Sweden)

    Bayram ÖZFIRAT

    2013-03-01

    Full Text Available As manuscript works, which constitute some indispensabletreasure with respect to history of our literature, come back into light,our knowledge on religious literary genres, as well as other, no doubtincreases. Such works added to literature thanks to efforts byresearchers give us the opportunity of close acquaintance with religiousworks that are considered as the most significant and the most prolificproducts of Divan Poetry, and of more accurate interpretationsregarding the genre of such works. Apart from works such as aqâid, fiqhand catechism (ilm-i hal in verse, siyer (life of Muhammad in verse,exegeses (tafsir and hadithes in verse that are generally written inpoetic structure for these disciplines, there are also some poetic piecesabout a subdivision or important question of abovementioned Islamicsciences. Faraiz, Hegira of Muhammad, miraj-name, rules of hajj,miracles of Prophet, all in verse, can be mentioned among suchexamples. In the wake of our researches, we found out that thesecatechisms in verse constitute a notable part of our religious literature.Tokatlı İshâk Efendi was an important poet and writer who lived in 17th century. Biographic sources dores not mention him alot. The poetryin rhymed couplets, Nazmu’l-Ulûm, which we are working on shows ushow important poet he is. At his Nazmu’l-Ulûm İshâk Efendi givesinformation about different sciences and wants them to learn. Becauseof these properties Nazmu’l-Ulûm is a written verse advice. Nazmu’l-Ulûm have got strong rhymes. That is possible to say poetry in rhymedcouplets have fascinating expressions rather than bare tellings. Somany telling arts and describtions were given place in that book. Tokatlı İshâk Efendi on yedinci yüzyılda yaşamış önemli bir şâir ve yazardır. Biyografik kaynaklar kendisinden fazla bahsetmez. Ama eserlerinden yola çıktığımızda Tokatlı İshâk Efendi’nin yetenekli bir şâir olduğunu rahatlıkla söyleyebiliriz. İncelediğimiz Nazmu’l-Ulûm adlı mesnevîsinde şâir, çeşitli ilimler hakkında bilgi verdikten sonra bunların öğrenilmesi gerektiğini vurgulamıştır. Mesnevî, arûz vezninin “Mefāīlün Mefāīlün Faūlün” kalıbıyla yazılmış olup 1098/1687 yılında Tokat’ta telif edilmiştir. Mesnevî toplam 422 beyittir. Araştırmalarımız neticesinde yirmi iki nüshasını tespit ettiğimiz mesnevîde çeşitli ilimler hakkında bilgi verildikten sonra bunların öğrenilmesi gereği vurgulanmaktadır. Nazmu’l-Ulûm bu yönüyle manzûm bir nasihatnâme olup öğrenilmesinin öğütlendiği ilimlerin bazıları şunlardır: 1. İlm-i tecvîd, 2. İlm-i Kelâm, 3. İlm-i Fıkıh, 4. İlm-i Tasavvuf ve Ahlâk, 5. İlm-i Hadîs, 6. İlm-i Tefsîr, 7. İlm-i Tıp, 8. İlm-i Lugat, 9. İlm-i Nahv ve Sarf, 10. İlm-i Mantık, 11. İlm-i Edeb, 12. İlm-i Usûl-i Fıkıh, 13. İlm-i Meânî, 14. İlm-i Hisâb, 15. İlm-i Hendese, 17. İlm-i Kîmyâ, 18. İlm-i Arûz, 19. İlm-i Eş’âr. Nazmu’l-Ulûm kafiye yönünden oldukça sağlamdır. Hatta eserin kafiye bakımından kusursuz bir yapıya sahip olduğunu söylemek de mümkündür. Mesnevîyi oluşturan kelimler dikkate alındığında Arapça ve Farsça kelimelerin çokluğu dikkat çekmektedir. Ama eserin bu özelliği onun akıcılığından bir şey kaybettirmemiştir. Mesnevîde her türlü kafiyeye rastlanmakla birlikte; en çok zengin ve tam kafiyelere yer verilmiş, az sayıda yarım kafiye kullanılmıştır. Mesnevîde, bolca söz sanatlarına ve tasvirlere yer verilmiştir. Gereksiz ifadelerden kaçınılmaya çalışılmıştır. İshâk Efendi arûz veznine ve dile hâkim bir şâirdir. Nazmu’l-Ulûm’da şâirin vezin kusuruna düşmeden dili rahatlıkla kullanması dikkat çekicidir. Bu özellik İshâk Efendi’nin şâirlik gücüne, çok sayıda şiir yazmasına ve yaşadığı dönem olan on yedinci yüzyıla değin Türkçe anlatımdaki gelişmeye de bağlıdır.

  4. Atividade antioxidante do extrato de sementes de limão (Citrus limon adicionado ao óleo de soja em teste de estocagem acelerada Antioxidant activity of lemon seed extract (Citrus limon added to soybean oil in accelerated incubator-storage test

    Directory of Open Access Journals (Sweden)

    Débora Maria Moreno Luzia

    2009-01-01

    Full Text Available The aim of this work was to evaluate antioxidant activity of lemon seeds added to soybean oil, submitted to accelerated incubator-storage test and to determine its synergistic effect with the synthetic antioxidant TBHQ. The treatments Control, TBHQ (50 mg/kg, LSE (2,400 mg/kg Lemon Seed Extract, Mixture 1 (LSE + 50 mg/kg TBHQ and Mixture 2 (LSE + 25 mg/kg TBHQ were prepared and subjected to the accelerated incubator-storage test at 60 ºC for 12 days; samples were taken every 3 days and analyzed regarding peroxide value and conjugated dienes. The results showed that antioxidant activity of the tested treatments were: TBHQ = Mixture 1 = Mixture 2 > LSE > Control.

  5. Melanite garnet-bearing nepheline syenite minor intrusion in Mawpyut ultramafic–mafic complex, Jaintia Hills, Meghalaya

    Indian Academy of Sciences (India)

    Monoj Maitra; J S David; S Bhaduri

    2011-12-01

    Mawpyut igneous suite in Jaintia Hills of Meghalaya plateau comprises differentiated suite of ultramafic–mafic rocks. The complex differs from other ultramafic–alkaline–carbonatite igneous emplacements of Shillong plateau and Mikir Hills like Jesra, Sung, Samchampi complexes, by the absence of alkaline–carbonatite rocks as major litho-units. Melanite garnet-bearing nepheline syenite, occurs as late phase minor intrusion in Mawpyut igneous complex, posseses alkaline character and shows inubiquitous relation with the host ultramafic–mafic rocks. On the other hand, this alkaline intrusive bodies of the Mawpyut igneous complex shows chemico-mineralogical resemblance with garnet-bearing nepheline syenite, ijolite litho-members of Jesra, Sung, Samchampi complexes of the region. It is interpreted that melanite garnet-bearing nepheline syenite intrusion in Mawpyut is contemporaneous with Jesra, Sung, Samchampi ultramafic–alkaline–carbonatite complexes and the host rocks of Mawpyut complex is an earlier magmatic activity possibly from a comparatively least enriched source.

  6. Trends in laboratory information management systems

    Energy Technology Data Exchange (ETDEWEB)

    Gibbon, G.A.

    1984-01-01

    Laboratory Information Management Systems (LIMS) are an outgrowth of laboratory automation, the increasing workload in most laboratories, and the success that Information Management Systems have shown in enhancing productivity in a wide variety of activities. One current need is a model for constructing a local area network for the LIMS.

  7. Discriminant Validity of the WISC-IV Culture-Language Interpretive Matrix

    Science.gov (United States)

    Styck, Kara M.; Watkins, Marley W.

    2014-01-01

    The Culture-Language Interpretive Matrix (C-LIM) was developed to help practitioners determine the validity of test scores obtained from students who are culturally and linguistically different from the normative group of a test. The present study used an idiographic approach to investigate the diagnostic utility of the C-LIM for the Wechsler…

  8. The Business of Politics Commerce, and Culture in East Asia

    DEFF Research Database (Denmark)

    Ooi, Can-Seng

    2016-01-01

    Review of: "Cultural Policies in East Asia: Dynamics Between the State, Arts and Creative Industries". Edited by Hye-Kyung Lee and Lorraine Lim. Basingstoke: Palgrave, Macmillan, 2014. 229 pp.......Review of: "Cultural Policies in East Asia: Dynamics Between the State, Arts and Creative Industries". Edited by Hye-Kyung Lee and Lorraine Lim. Basingstoke: Palgrave, Macmillan, 2014. 229 pp....

  9. Structure Characterization and Lead Detoxification Effect of Carboxymethylated Melanin Derived from Lachnum Sp.

    Science.gov (United States)

    Zong, Shuai; Li, Lan; Li, Jinglei; Shaikh, Farnaz; Yang, Liu; Ye, Ming

    2017-06-01

    In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined according to the results of Fourier transform infrared (FT-IR), (1)H NMR, (13)C NMR, and pyrolysis/GC-MS analysis. With the aim to increase its water solubility, its carboxymethylated derivative, named CLIM205, was formed by the substitution of hydrogen atoms in LIM205 with one, two, and three carboxymethylate groups. FT-IR, UV, and ESI-MS analysis demonstrated that the carboxymethylate groups were conjugated onto LIM205. The lead detoxification activities of LIM205 and CLIM205 had also been investigated. In vivo test showed that both LIM205 and CLIM205 reduced the tissue lead concentration, enhanced lead excretion, and reversed lead-induced alterations in superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) concentrations in mice, with CLIM205 showed better efficacy. The study indicates that LIM205 and CLIM205 have significant lead detoxification effect which will contribute to solve related problems.

  10. Dicty_cDB: Contig-U14145-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available H dependent difla... 82 5e-15 AB506804_1( AB506804 |pid:none) Lehmannia valentiana limNOS2 mRNA ... 82 5e-15... 72 6e-12 AB333805_1( AB333805 |pid:none) Lehmannia valentiana limNOS mRNA f... 72 6e-12 CP000109_1890( CP00

  11. A Case Study of Singapore’s Counterinsurgency Strategy: An Analysis using the Tools of Operational Design

    Science.gov (United States)

    2011-04-01

    recalcitrant and even seek revenge against witnesses and AU/ACSC/LIM/AY11 27 juries. Culturally, Asians are superstitious and they had an aversion to...ACSC/LIM/AY11 44 The jury system did not work in Singapore. Asian superstitious beliefs and a general reluctance to take responsibility for

  12. A Remark on the Omori-Yau Maximum Principle

    CERN Document Server

    Borbely, Albert

    2012-01-01

    A Riemannian manifold $M$ is said to satisfy the Omori-Yau maximum principle if for any $C^2$ bounded function $g:M\\to \\Bbb R$ there is a sequence $x_n\\in M$, such that $\\lim_{n\\to \\infty}g(x_n)=\\sup_M g$, $ \\lim_{n\\to \\infty}|\

  13. A Positive Approach to Change: The Role of Appreciative Inquiry in Library and Information Organisations

    Science.gov (United States)

    Kelly, Tricia

    2010-01-01

    Library and information management (LIM) organisations are on an almost continual path of change driven by changes in technology, service models, staffing structures, and financial allocations. The way in which LIM organisations approach change varies, as does the success rate of change management procedures undertaken. One particular approach to…

  14. A Positive Approach to Change: The Role of Appreciative Inquiry in Library and Information Organisations

    Science.gov (United States)

    Kelly, Tricia

    2010-01-01

    Library and information management (LIM) organisations are on an almost continual path of change driven by changes in technology, service models, staffing structures, and financial allocations. The way in which LIM organisations approach change varies, as does the success rate of change management procedures undertaken. One particular approach to…

  15. Seasonal and decadal forecasts of Atlantic Sea surface temperatures using a linear inverse model

    Science.gov (United States)

    Huddart, Benjamin; Subramanian, Aneesh; Zanna, Laure; Palmer, Tim

    2017-09-01

    Predictability of Atlantic Ocean sea surface temperatures (SST) on seasonal and decadal timescales is investigated using a suite of statistical linear inverse models (LIM). Observed monthly SST anomalies in the Atlantic sector (between 22°S and 66°N) are used to construct the LIMs for seasonal and decadal prediction. The forecast skills of the LIMs are then compared to that from two current operational forecast systems. Results indicate that the LIM has good forecast skill for time periods of 3-4 months on the seasonal timescale with enhanced predictability in the spring season. On decadal timescales, the impact of inter-annual and intra-annual variability on the predictability is also investigated. The results show that the suite of LIMs have forecast skill for about 3-4 years over most of the domain when we use only the decadal variability for the construction of the LIM. Including higher frequency variability helps improve the forecast skill and maintains the correlation of LIM predictions with the observed SST anomalies for longer periods. These results indicate the importance of temporal scale interactions in improving predictability on decadal timescales. Hence, LIMs can not only be used as benchmarks for estimates of statistical skill but also to isolate contributions to the forecast skills from different timescales, spatial scales or even model components.

  16. Portraits of life: Patterns of events over the lifespan

    NARCIS (Netherlands)

    Schroots, J.J.F.; Assink, M.H.J.

    2005-01-01

    This explorative content-analytic study completes earlier studies on the lifespan distributions of number and affect of past and future life-events, collected by means of the Life-line Interview Method (LIM), for three age groups of men and women (young, middle and late adulthood). LIM events were c

  17. Auswirkungen des Urin-pH auf das Ballonvolumen bei Dauerkathetern

    OpenAIRE

    Pannek J; Weiß D

    2007-01-01

    Einleitung: Eine häufige Komplikation der transurethralen oder suprapubischen Dauerkatheterversorgung ist die Dislokation durch eine insuffiziente Katheterblockung. Die Auswirkungen des Urin-pH auf das Ballonvolumen sind dabei bisher nicht untersucht. Methodik: In einer In-vitro-Studie wurden Dauerkatheter aus Silikon und Latex mit entweder 10%iger Glyzerinlösung oder mit 0,9%iger NaCl-Lösung geblockt und 14 Tage bei verschiedenen pH-Werten in künstlichem Urin gelagert. Nach diesem Zeitraum ...

  18. Dynamic Performance of Subway Vehicle with Linear Induction Motor System

    Science.gov (United States)

    Wu, Pingbo; Luo, Ren; Hu, Yan; Zeng, Jing

    The light rail vehicle with Linear Induction Motor (LIM) bogie, which is a new type of urban rail traffic tool, has the advantages of low costs, wide applicability, low noise, simple maintenance and better dynamic behavior. This kind of vehicle, supported and guided by the wheel and rail, is not driven by the wheel/rail adhesion force, but driven by the electromagnetic force between LIM and reaction plate. In this paper, three different types of suspensions and their characteristic are discussed with considering the interactions both between wheel and rail and between LIM and reaction plate. A nonlinear mathematical model of the vehicle with LIM bogie is set up by using the software SIMPACK, and the electromechanical model is also set up on Simulink roof. Then the running behavior of the LIM vehicle is simulated, and the influence of suspension on the vehicle dynamic performance is investigated.

  19. Electromagnetic Performance Calculation of HTS Linear Induction Motor for Rail Systems

    Science.gov (United States)

    Liu, Bin; Fang, Jin; Cao, Junci; Chen, Jie; Shu, Hang; Sheng, Long

    2017-07-01

    According to a high temperature superconducting (HTS) linear induction motor (LIM) designed for rail systems, the influence of electromagnetic parameters and mechanical structure parameters on the electromagnetic horizontal thrust, vertical force of HTS LIM and the maximum vertical magnetic field of HTS windings are analyzed. Through the research on the vertical field of HTS windings, the development regularity of the HTS LIM maximum input current with different stator frequency and different thickness value of the secondary conductive plate is obtained. The theoretical results are of great significance to analyze the stability of HTS LIM. Finally, based on theory analysis, HTS LIM test platform was built and the experiment was carried out with load. The experimental results show that the theoretical analysis is correct and reasonable.

  20. Are we near the predictability limit of tropical Indo-Pacific sea surface temperatures?

    Science.gov (United States)

    Newman, Matthew; Sardeshmukh, Prashant D.

    2017-08-01

    The predictability of seasonal anomalies worldwide rests largely on the predictability of tropical sea surface temperature (SST) anomalies. Tropical forecast skill is also a key metric of climate models. We find, however, that despite extensive model development, the tropical SST forecast skill of the operational North American Multi-Model Ensemble (NMME) of eight coupled atmosphere-ocean models remains close both regionally and temporally to that of a vastly simpler linear inverse model (LIM) derived from observed covariances of SST, sea surface height, and wind fields. The LIM clearly captures the essence of the predictable SST dynamics. The NMME and LIM skills also closely track and are only slightly lower than the potential skill estimated using the LIM's forecast signal-to-noise ratios. This suggests that the scope for further skill improvement is small in most regions, except in the western equatorial Pacific where the NMME skill is currently much lower than the LIM skill.