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Sample records for sunfish f1 hybrids

  1. Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.

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    Diogo Teruo Hashimoto

    Full Text Available Juvenile fish trade monitoring is an important task on Brazilian fish farms. However, the identification of juvenile fish through morphological analysis is not feasible, particularly between interspecific hybrids and pure species individuals, making the monitoring of these individuals difficult. Hybrids can be erroneously identified as pure species in breeding facilities, which might reduce production on farms and negatively affect native populations due to escapes or stocking practices. In the present study, we used a multi-approach analysis (molecular and cytogenetic markers to identify juveniles of three serrasalmid species (Colossoma macropomum, Piaractus mesopotamicus and Piaractus brachypomus and their hybrids in different stocks purchased from three seed producers in Brazil. The main findings of this study were the detection of intergenus backcrossing between the hybrid ♀ patinga (P. mesopotamicus×P. brachypomus×♂ C. macropomum and the occurrence of one hybrid triploid individual. This atypical specimen might result from automixis, a mechanism that produces unreduced gametes in some organisms. Moreover, molecular identification indicated that hybrid individuals are traded as pure species or other types of interspecific hybrids, particularly post-F1 individuals. These results show that serrasalmid fish genomes exhibit high genetic heterogeneity, and multi-approach methods and regulators could improve the surveillance of the production and trade of fish species and their hybrids, thereby facilitating the sustainable development of fish farming.

  2. Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower

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    Terzić Sreten

    2006-01-01

    Full Text Available Phenotype, chromosomes pairing and pollen vitality were compared between parental populations and F1 hybrids of interspecific cross between Helianthus annuus L. and cultivated sunflower. The investigation of the simple sequence repeats (SSR polymorphism was also used to test the hybrid nature of F1 populations. The phenotypic traits of F1 hybrid plants were either closer to the wild species or intermediate. Irregular chromosome pairing was found in only 0 to 10% of meiocytes in the meiosis of F1 hybrid plants. Interspecific crosses were confirmed with SSR markers in all hybrid combinations. Alleles that were not present in parental DNA were frequently observed in F1 hybrids. That is additional evidence that those hybrid combinations were not produced by self-fertilization. The results suggest that SSR markers can be efficiently used for the F1 hybrid characterization in crosses between closely related species, in which, the changes of phenotype, meiosis and pollen vitality are not always significant.

  3. Segregation ratios of colored grains in F1 hybrid wheat

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    Zifeng Guo

    2012-01-01

    Full Text Available Nutritious and functional foods from wheat have received great attention in recent years. Colored-grain wheat contains a large number of nutrients such as anthocyanins and hence the breeding is interesting. In this work, colored-grained wheat lines of mixed pollination of einkorn wheat (Triticum boeoticum, AA and French rye (French Secale cereale, RR were used as male parents and wheat line Y1642 (derived from common wheat and Agropyron elongatum, AABBDD was used as the female parent. These colored wheat were used for diallel cross to study the segregation ratios of F1 colored grains. Results show that the color inheritance of purple-grained wheat follows a maternal inheritance pattern and that the blue-grained wheat expresses xenia in most cases. In some circumstances, the grains with different color shades appear in the same spike.

  4. Suppression of F1 Male-Specific Lethality in Caenorhabditis Hybrids by cbr-him-8

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    Vaishnavi Ragavapuram

    2016-03-01

    Full Text Available Haldane’s Rule and Darwin’s Corollary to Haldane’s Rule are the observations that heterogametic F1 hybrids are frequently less fit than their homogametic siblings, and that asymmetric results are often obtained from reciprocal hybrid crosses. In Caenorhabditis, Haldane’s Rule and Darwin’s Corollary have been observed in several hybrid crosses, including crosses of Caenorhabditis briggsae and C. nigoni. Fertile F1 females are obtained from reciprocal crosses. However, F1 males obtained from C. nigoni mothers are sterile and F1 males obtained from C. briggsae die during embryogenesis. We have identified cbr-him-8 as a recessive maternal-effect suppressor of F1 hybrid male-specific lethality in this combination of species. This result implicates epigenetic meiotic silencing in the suppression of F1 male-specific lethality. It is also shown that F1 males bearing a C. briggsae X chromosome are fertile. When crossed to C. briggsae hermaphrodites or F1 females derived from C. briggsae hermaphrodites, viable F2 and backcross (B2 progeny were obtained. Sibling males that possessed a C. nigoni X chromosome were sterile. Therefore, the sterility of F1 males bearing a C. nigoni X chromosome must result from dysgenic interactions between the X chromosome of C. nigoni and the autosomes of C. briggsae. The fertility of F1 males bearing a C. briggsae X chromosome provides an opportunity to identify C. nigoni loci that prevent spermatogenesis, and hence hermaphroditic reproduction, in diplo-X hybrids.

  5. Chromosomal rearrangements directly cause underdominant F1 pollen sterility in Mimulus lewisii-Mimulus cardinalis hybrids.

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    Stathos, Angela; Fishman, Lila

    2014-11-01

    Chromosomal rearrangements can contribute to the evolution of postzygotic reproductive isolation directly, by disrupting meiosis in F1 hybrids, or indirectly, by suppressing recombination among genic incompatibilities. Because direct effects of rearrangements on fertility imply fitness costs during their spread, understanding the mechanism of F1 hybrid sterility is integral to reconstructing the role(s) of rearrangements in speciation. In hybrids between monkeyflowers Mimulus cardinalis and Mimulus lewisii, rearrangements contain all quantitative trait loci (QTLs) for both premating barriers and pollen sterility, suggesting that they may have facilitated speciation in this model system. We used artificial chromosome doubling and comparative mapping to test whether heterozygous rearrangements directly cause underdominant male sterility in M. lewisii-M. cardinalis hybrids. Consistent with a direct chromosomal basis for hybrid sterility, synthetic tetraploid F1 s showed highly restored fertility (83.4% pollen fertility) relative to diploids F1 s (36.0%). Additional mapping with Mimulus parishii-M. cardinalis and M. parishii-M. lewisii hybrids demonstrated that underdominant male sterility is caused by one M. lewisii specific and one M. cardinalis specific reciprocal translocation, but that inversions had no direct effects on fertility. We discuss the importance of translocations as causes of reproductive isolation, and consider models for how underdominant rearrangements spread and fix despite intrinsic fitness costs.

  6. Little qualitative RNA misexpression in sterile male F1 hybrids of Drosophila pseudoobscura and D. persimilis

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    Noor Mohamed AF

    2002-09-01

    Full Text Available Abstract Background Although the genetics of hybrid sterility has been the subject of evolutionary studies for over sixty years, no one has shown the reason(s why alleles that operate normally within species fail to function in another genetic background. Several lines of evidence suggest that failures in normal gene transcription contribute to hybrid dysfunctions, but genome-wide studies of gene expression in pure-species and hybrids have not been undertaken. Here, we study genome-wide patterns of expression in Drosophila pseudoobscura, D. persimilis, and their sterile F1 hybrid males using differential display. Results Over five thousand amplifications were analyzed, and 3312 were present in amplifications from both of the pure species. Of these, 28 (0.5% were not present in amplifications from adult F1 hybrid males. Using product-specific primers, we were able to confirm one of nine of the transcripts putatively misexpressed in hybrids. This transcript was shown to be male-specific, but without detectable homology to D. melanogaster sequence. Conclusion We tentatively conclude that hybrid sterility can evolve without widespread, qualitative misexpression of transcripts in species hybrids. We suggest that, if more misexpression exists in sterile hybrids, it is likely to be quantitative, tissue-specific, and/ or limited to earlier developmental stages. Although several caveats apply, this study was a first attempt to determine the mechanistic basis of hybrid sterility, and one potential candidate gene has been identified for further study.

  7. Mona F1: New pepper (Capsicum annuum L. hybrid in the Centre for Vegetable Crops

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    Cvikić Dejan

    2007-01-01

    Full Text Available The planted area various ways of pepper consumption (fresh or processed, make pepper one of the most important cultivars in vegetable breeding. In our country, up until now, the producers have usually grown varieties and domestic populations of pepper, while in more developed countries the usage of F1 hybrids is much more popular. The first pepper hybrids have been created in the Centre for Vegetable Crops by crossing new lines with male sterility gene ms-3 and selected genotypes from pepper collection. Created hybrids have higher yield, quality fruits and early ripening. This paper is the result of comparative trial in controlled conditions. Pepper varieties Župska rana, Zlatna medalja, Palanačka kapija and Duga bela, as well as new hybrid Mona F1 were the research matherial in order to observe the most important pepper traits.

  8. Dissecting the genetic architecture of F1 hybrid sterility in house mice.

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    Dzur-Gejdosova, Maria; Simecek, Petr; Gregorova, Sona; Bhattacharyya, Tanmoy; Forejt, Jiri

    2012-11-01

    Hybrid sterility as a postzygotic reproductive isolation mechanism has been studied for over 80 years, yet the first identifications of hybrid sterility genes in Drosophila and mouse are quite recent. To study the genetic architecture of F(1) hybrid sterility between young subspecies of house mouse Mus m. domesticus and M. m. musculus, we conducted QTL analysis of a backcross between inbred strains representing these two subspecies and probed the role of individual chromosomes in hybrid sterility using the intersubspecific chromosome substitution strains. We provide direct evidence that the asymmetry in male infertility between reciprocal crosses is conferred by the middle region of M. m. musculus Chr X, thus excluding other potential candidates such as Y, imprinted genes, and mitochondrial DNA. QTL analysis identified strong hybrid sterility loci on Chr 17 and Chr X and predicted a set of interchangeable autosomal loci, a subset of which is sufficient to activate the Dobzhansky-Muller incompatibility of the strong loci. Overall, our results indicate the oligogenic nature of F(1) hybrid sterility, which should be amenable to reconstruction by proper combination of chromosome substitution strains. Such a prefabricated model system should help to uncover the gene networks and molecular mechanisms underlying hybrid sterility.

  9. Types of tree growth and fruit setting in F1 apple hybrids

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    Radu E. SESTRAS

    1998-08-01

    Full Text Available 1656 F1 hybrid apple seedlings, belonging to 127 combinations, have been screened according to their growing and fruit setting types, as it was phenotypically expressed. LESPINASSE (1977; 1992 amalgamated these two traits into a single one which was named ";ideotype";. The screened F1 individuals have been considered as resembling one of the following four architectural ideotypes of the trees indicated by Lespinasse: columnar, spur, standard and weeping. Different ratios of spur, standard and columnar F1 individuals were obtained depending on genitors and on the fact that a certain genitor had been used as a maternal or paternal partner in direct/reciprocal crosses. The monogenic inheritance of the columnar ideotype, proposed by Kelsey and Brown (1992; Lane (1992, does not seem to be the only genetic mechanism involved in the inheritance of this trait. Our experimental results suggest the polygenic determination of this ideotype as more probable than the monogenic one.

  10. Spatial reference memory in normal aging Fischer 344 × Brown Norway F1 hybrid rats.

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    McQuail, Joseph A; Nicolle, Michelle M

    2015-01-01

    Fischer 344 × Brown Norway F1 (F344 × BN-F1) hybrid rats express greater longevity with improved health relative to aging rodents of other strains; however, few behavioral reports have thoroughly evaluated cognition across the F344 × BN-F1 lifespan. Consequently, this study evaluated spatial reference memory in F344 × BN-F1 rats at 6, 18, 24, or 28 months of age in the Morris water maze. Reference memory decrements were observed between 6 and 18 months and 18 and 24 months. At 28 months, spatial learning was not worse than 24 months, but swim speed was significantly slower. Reliable individual differences revealed that ∼50% of 24- to 28-month-old rats performed similarly to 6 months, whereas others were spatial learning impaired. Aged rats were impaired at learning within daily training sessions but not impaired at retaining information between days of training. Aged rats were also slower to learn to escape onto the platform, regardless of strategy. In summary, these data clarify the trajectory of cognitive decline in aging F344 × BN-F1 rats and elucidate relevant behavioral parameters.

  11. Morphological, yield, cytological and molecular characterization of a bread wheat × tritordeum F1 hybrid

    Indian Academy of Sciences (India)

    J. Lima-Brito; A. Carvalho; A. Martin; J. S. Heslop-Harrison; H. Guedes-Pinto

    2006-08-01

    The morphological, yield, cytological and molecular characteristics of bread wheat × tritordeum F1 hybrids ($2n = 6x = 42$; AABBDHch) and their parents were analysed. Morphologically, these hybrids resembled the wheat parent. They were slightly bigger than both parents, had more spikelets per spike, and tillered more profusely. The hybrids are self-fertile but a reduction of average values of yield parameters was observed. For the cytological approach we used a double-target fluorescence in situ hybridization performed with total genomic DNA from Hordeum chilense L. and the ribosomal sequence pTa71. This technique allowed us to confirm the hybrid nature and to analyse chromosome pairing in this material. Our results showed that the expected complete homologous pairing (14 bivalents plus 14 univalents) was only observed in 9.59% of the pollen mother cells (PMCs) analysed. Some PMCs presented autosyndetic pairing of Hch and A, B or D chromosomes. The average number of univalents was higher in the wheat genome (6.8) than in the Hch genome (5.4). The maximum number of univalents per PMC was 20. We only observed wheat multivalents (one per PMC) but the frequency of trivalents (0.08) was higher than that of quadrivalents (0.058). We amplified 50 RAPD bands polymorphic between the F1 hybrid and one of its parents, and 31 ISSR polymorphic bands. Both sets of markers proved to be reliable for DNA fingerprinting. The complementary use of morphological and yield analysis, molecular cytogenetic techniques and molecular markers allowed a more accurate evaluation and characterization of the hybrids analysed here.

  12. Reduced sexual compatibility between cultivated and wild chicory and their F1 hybrids

    DEFF Research Database (Denmark)

    Hauser, T.P.; Bagger Jørgensen, Rikke; Toneatto, F.

    2012-01-01

    Crops were domesticated from wild plants not too long ago and have subsequently diverged from the wild ones, especially in traits used by humans. Whether divergence between the cultigen and wild forms has also lead to reduced reproductive compatibility is unknown for many species. Chicory...... (Cichorium intybus L.) has been bred as a crop at least since Roman times. To test if this has led to a loss in reproductive compatibility with wild chicory, we planted cultivar, wild, and F1 hybrid plants into two field plots, and let them pollinate freely. On 2 days, in the beginning and middle...... of the flowering season, we counted the numbers of flowering capitula and open flowers per capitulum, which in combination with counts of viable pollen per flower were used to estimate the expected proportion of seeds fathered by cultivar, wild, and hybrid plants. Open capitula on wild and hybrid plants were...

  13. Mode of inheritance for fruit firmness in tomato hybrids of F1 generation (Lycoperscum esculentum Mill.

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    Sušić Zoran

    2000-01-01

    Full Text Available Present day program for tomato selection are aimed at creating the genotypes with firm fruit. The fruits with this quality surfer from minor injuries while being harvested and transported, which directly affects their better consumption purpose. By crossing seven divergent tomato genotypes that differed among themselves in fruit firmness, and by applying the method of full diallel without reciprocal crossings, we obtained 21 hybrids of F1 generation. Upon analyzing the components of the genetic variance we found out that dominant genes prevailed in inheriting this feature. Considering all the crossing combinations together, it could be concluded that super dominance was the mode of inheritance recorded in Fl generation. The hybrid combination obtained by crossing the two hybrids with the best general combining ability (V-100 x No-10 was characterized by the best specific combining ability. .

  14. Use of hybridization (F1 in forage sorghum (Sorghum bicolor (L. Moench breeding

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    Pataki Imre

    2010-01-01

    Full Text Available In plants with bisexual flowers, the development of hybrids and F1 seed production is only possible by using cytoplasmatic male sterility. The discovery of such sterility and the maintainers has made it possible to utilize the phenomenon of heterosis to improve yields and yield components in forage sorghum. It has been shown that the best way to develop forage sorghum hybrids is to cross grain sorghum as the female parent and Sudan grass as the male. The objective of this study was to develop a forage sorghum hybrid for the production of green matter to be used either fresh or for silage. The sorghum hybrid developed in these efforts (Siloking is intended for multiple cutting, as the basal nodes produce buds and regrowth takes place. The performance of the new hybrid with respect to yield and quality was compared to that of the forage sorghum cultivar NS Džin. In a two-year study conducted under different growing conditions in four locations, Siloking produced an average green matter yield of 86.29 t ha-1 (two cuts, a dry matter yield of 25.34 t ha-1, and a crude protein content of 11.85 %. Siloking outperformed NS Džin in terms of yield and quality. .

  15. F1 hybrid of cultivated apple (Malus x domestica) and European pear (Pyrus communis) with fertile F2 offspring

    NARCIS (Netherlands)

    Fischer, T.C.; Malnoy, M.; Hofmann, T.; Schwab, W.; Palmieri, L.; Wehrens, H.R.M.J.; Schuch, L.A.; Müller, M.; Schimmelpfeng, H.; Velasco, R.; Martens, S.

    2014-01-01

    The establishment of intergeneric hybrids for horticultural and agricultural crops is still a demanding task for breeding programmes. The aim of such approaches is to introduce new quality and resistance traits and to enlarge the gene pool. Recently, an F1 hybrid between Malus × domestica and Pyrus

  16. A hybrid toxin from bacteriophage f1 attachment protein and colicin E3 has altered cell receptor specificity.

    OpenAIRE

    Jakes, K S; Davis, N G; Zinder, N D

    1988-01-01

    A hybrid protein was constructed in vitro which consists of the first 372 amino acids of the attachment (gene III) protein of filamentous bacteriophage f1 fused, in frame, to the carboxy-terminal catalytic domain of colicin E3. The hybrid toxin killed cells that had the F-pilus receptor for phage f1 but not F- cells. The activity of the hybrid protein was not dependent upon the presence of the colicin E3 receptor, BtuB protein. The killing activity was colicin E3 specific, since F+ cells expr...

  17. Degree of inheritance and heritability of yield in parental genotypes and F1 hybrids of tobacco

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    Aleksoski Jane A.

    2011-01-01

    Full Text Available The mode and level of inheritance of green and dry mass yield per stalk were investigated in four parental genotypes (Burley - B 2/93, Suchum - S1, Suchum - S2 and Prilep - P-84 and in their six diallel F1 hybrids. The trial was set up in 2007, 2008 and 2009 in the field of Tobacco Institute-Prilep in a randomized block design with four replications. The aim of the investigation was to estimate the heritability as an indicator of the inheritance of the yield as one of the most important quantitative characters of tobacco, in order to give suggestions for the selection of parental genotypes and directions for the creation of new varieties. The mode of inheritance was estimated according to the test - significance of the mean value of F1 progeny compared to the parental average. Narrow-sense heritability was estimated after Allard (1960, while broad-sense heritability and genetic components were estimated after Mather and Jinks (1974. The mode of inheritance in the hybrids was different. Positive heterosis for green and dry mass yields per stalk was recorded in S1 x S2. Negative heterosis for green mass yields per stalk was recorded in S1 x P-84 and S2 x P-84, while for dry mass yield it was recorded in S1 x P-84. Inheritance of the characters during the three years of investigation was identical. The higher heritability index of both types was recorded for dry mass yield. As regards inheritance of the yield, the values of broad-sense heritability were higher than those of narrow-sense heritability.

  18. Borders and comparative cytoarchitecture of the perirhinal and postrhinal cortices in an F1 hybrid mouse.

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    Beaudin, Stephane A; Singh, Teghpal; Agster, Kara L; Burwell, Rebecca D

    2013-02-01

    We examined the cytoarchitectonic and chemoarchitectonic organization of the cortical regions associated with the posterior rhinal fissure in the mouse brain, within the framework of what is known about these regions in the rat. Primary observations were in a first-generation hybrid mouse line, B6129PF/J1. The F1 hybrid was chosen because of the many advantages afforded in the study of the molecular and cellular bases of learning and memory. Comparisons with the parent strains, the C57BL6/J and 129P3/J are also reported. Mouse brain tissue was processed for visualization of Nissl material, myelin, acetyl cholinesterase, parvalbumin, and heavy metals. Tissue stained for heavy metals by the Timm's method was particularly useful in the assignment of borders and in the comparative analyses because the patterns of staining were similar across species and strains. As in the rat, the areas examined were parcellated into 2 regions, the perirhinal and the postrhinal cortices. The perirhinal cortex was divided into areas 35 and 36, and the postrhinal cortex was divided into dorsal (PORd) and ventral (PORv) subregions. In addition to identifying the borders of the perirhinal cortex, we were able to identify a region in the mouse brain that shares signature features with the rat postrhinal cortex.

  19. The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance in F1 Hybrids

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    Tomoko Takamiya

    2009-01-01

    Full Text Available We analyzed inheritance of DNA methylation in reciprocal F1 hybrids (subsp. japonica cv. Nipponbare × subsp. indica cv. Kasalath of rice (Oryza sativa L. using restriction landmark genome scanning (RLGS, and detected differing RLGS spots between the parents and reciprocal F1 hybrids. MspI/HpaII restriction sites in the DNA from these different spots were suspected to be heterozygously methylated in the Nipponbare parent. These spots segregated in F1 plants, but did not segregate in selfed progeny of Nipponbare, showing non-Mendelian inheritance of the methylation status. As a result of RT-PCR and sequencing, a specific allele of the gene nearest to the methylated sites was expressed in reciprocal F1 plants, showing evidence of biased allelic expression. These results show the applicability of RLGS for scanning of non-Mendelian inheritance of DNA methylation and biased allelic expression.

  20. Gamete fertility and ovule number variation in selfed reciprocal F1 hybrid triploid plants are heritable and display epigenetic parent-of-origin effects.

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    Duszynska, Dorota; McKeown, Peter C; Juenger, Thomas E; Pietraszewska-Bogiel, Anna; Geelen, Danny; Spillane, Charles

    2013-04-01

    Polyploidy and hybridization play major roles in plant evolution and reproduction. To investigate the reproductive effects of polyploidy and hybridization in Arabidopsis thaliana, we analyzed fertility of reciprocal pairs of F1 hybrid triploids, generated by reciprocally crossing 89 diploid accessions to a tetraploid Ler-0 line. All F1 hybrid triploid genotypes exhibited dramatically reduced ovule fertility, while variation in ovule number per silique was observed across different F1 triploid genotypes. These two reproductive traits were negatively correlated suggesting a trade-off between increased ovule number and ovule fertility. Furthermore, the ovule fertility of the F1 hybrid triploids displayed both hybrid dysgenesis and hybrid advantage (heterosis) effects. Strikingly, both reproductive traits (ovule fertility, ovule number) displayed epigenetic parent-of-origin effects between genetically identical reciprocal F1 hybrid triploid pairs. In some F1 triploid genotypes, the maternal genome excess F1 hybrid triploid was more fertile, whilst for other accessions the paternal genome excess F1 hybrid triploid was more fertile. Male gametogenesis was not significantly disrupted in F1 triploids. Fertility variation in the F1 triploid A. thaliana is mainly the result of disrupted ovule development. Overall, we demonstrate that in F1 triploid plants both ovule fertility and ovule number are subject to parent-of-origin effects that are genome dosage-dependent.

  1. Nitrogen metabolism enzymes, soluble protein and free proline content in soybean genotypes and their F1 hybrids

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    Kereši Sanja T.

    2008-01-01

    Full Text Available Nitrate reductase and glutamine synthetase activity, as well as free proline and soluble protein content were measured in eight soybean parent genotypes and six F1 hybrids. The aim of this study was to determine variability and the mode of inheritance for these parameters, and point out the genotypes of interest for future breeding programs. Analysed genotypes and their F1 hybrids expressed significant differences in activities of nitrate reductase and glutamine synthetase enzymes, as well as in soluble proteins and free proline contents. Since mode of inheritance for all investigated traits was in most cases dominance or heterosis, it can be concluded that these parameters are under control of dominant genes. The obtained results suggest that genotypes with favorable traits, such as variety Linda, line 1511, and F1 hybrids (Linda x LN92-7369 and (Balkan x BL-8, could be of interest as a good starting material for further breeding programs.

  2. Ribosomal protein genes are highly enriched among genes with allele-specific expression in the interspecific F1 hybrid catfish.

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    Chen, Ailu; Wang, Ruijia; Liu, Shikai; Peatman, Eric; Sun, Luyang; Bao, Lisui; Jiang, Chen; Li, Chao; Li, Yun; Zeng, Qifan; Liu, Zhanjiang

    2016-06-01

    Interspecific hybrids provide a rich source for the analysis of allele-specific expression (ASE). In this work, we analyzed ASE in F1 hybrid catfish using RNA-Seq datasets. While the vast majority of genes were expressed with both alleles, 7-8 % SNPs exhibited significant differences in allele ratios of expression. Of the 66,251 and 177,841 SNPs identified from the datasets of the liver and gill, 5420 (8.2 %) and 13,390 (7.5 %) SNPs were identified as significant ASE-SNPs, respectively. With these SNPs, a total of 1519 and 3075 ASE-genes were identified. Gene Ontology analysis revealed that genes encoding cytoplasmic ribosomal proteins (RP) were highly enriched among ASE genes. Parent-of-origin was determined for 27 and 30 ASE RP genes in the liver and gill, respectively. The results indicated that genes from both channel catfish and blue catfish were involved in ASE. However, each RP gene appeared to be almost exclusively expressed from only one parent, indicating that ribosomes in the hybrid catfish were in the "hybrid" form. Overall representation of RP transcripts among the transcriptome appeared lower in the F1 hybrid catfish than in channel catfish or blue catfish, suggesting that the "hybrid" ribosomes may work more efficiently for translation in the F1 hybrid catfish.

  3. Early growth performance of full-sib Acacia auriculiformis x Acacia mangium F1 hybrid progenies at three different sites

    Science.gov (United States)

    Shah Aimin, Atirah Abdullah; Abdullah, Mohd Zaki; Muhammad, Norwati; Ratnam, Wickneswari

    2014-09-01

    Field trials of 14 full sib Acacia auriculiformis x Acacia mangium F1 hybrid progenies were evaluated for growth performance at three sites (Bintulu, Mentakab and Segamat). Results indicated that there were significant differences (p> 0.05) for diameter breast height (Dbh) and total height (Ht) among the progenies and different sites. Superior progenies have been identified for future tree selection and improvement.

  4. Correlation Analysis between Body Size and Slaughter Performance in F-1 Hybrid Offspring of Princess Chicken and Kirin Chicken

    Institute of Scientific and Technical Information of China (English)

    Li; Naibin; Du; Bingwang; Yang; Fenxia; Tao; Lin; Chen; Jiebo

    2014-01-01

    In order to study the meat development value of princess chicken,the body size traits and slaughter performance of 12-week-old F1 hybrid offspring of princess chicken(♂) and kirin chicken(♀) were measured and the correlations between different traits were analyzed. The results showed that body length,keel length and shank length of male F1 hybrid offspring were significantly higher than those of female chickens(P < 0. 05). The live weight,carcass weight,semi-eviscerated weight,semi-eviscerated ratio,eviscerated weight,chest muscle weight,the leg muscle weight and heart weight of male chickens were extremely significantly higher than that of female chickens(P < 0. 01),and the leg muscle ratio and wings weight were significantly higher than that of female chickens(P < 0. 05),but sebum thickness of male chickens was extremely significantly lower than that of female chickens(P < 0. 01). Other indicators failed to reach the significant difference level. There were extremely significant or significant correlations between the slaughter performance and body size in F1 hybrid offspring. The regression equations between different indicators were identified and developed. The results provided a certain theoretical reference to predict slaughter performance indicators through a living body size measurement,and revealed an improved production performance of F1 hybrid offspring.

  5. 'Caro-Tex 312’ – An F1 Hybrid, High Yielding, Multiple Disease Resistant, Orange Habanero Pepper Cultivar

    Science.gov (United States)

    Texas A&M University and the USDA-ARS U.S. Vegetable Laboratory in Charleston, SC, have developed a new, F1 hybrid Habanero pepper cultivar. ‘Caro-Tex 312’ produces a large, orange-fruited Habanero pepper with typical shape and high pungency. It also possesses unique yield, early maturity and dise...

  6. On the Hybrid F1 Characteristics of Physiology, Biochemistry, Product Quality and Resistance to Black Shank in Oriental Tobacco

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    Chen X

    2014-12-01

    Full Text Available Experiments were conducted from 1996 to 1998 at the Hefei Institute of Economics and Technology and at the Oriental Experimental Station of the Zhejiang Province (China. Seven F1 hybrids and three parental varieties of Oriental tobaccos were evaluated for the characteristics of photosynthetic and transpiration rates, esterase isozymes, resistance to black shank, quality and product potential from the 1996-1998 growing seasons. Tobacco leaves had higher photosynthetic rates and many differences among genotypes in the early stage of plant vigorous growth compared with more mature leaves. However, transpiration rates were lower in the younger leaves and greater in the more mature leaves. All the entries had four common bands (B1, B3, B4 and B6 of the esterase isoenzymes. Differences between entries resulted from in having or not having the B2 and B5 bands and color intensity differences of all the bands. These differences could be used to identify individual entries. The F1hybrids Samsun X Toy and Samsun X Argjiro, compared with the CK Samsun control, had obvious heterotic vigor in the characteristics of product, for yield, quality and resistance to black shank. The F1 hybrid Samsun X Toy maintained higher photosynthetic and transpiration rates in the two growth stages compared to other entries. However, the F1hybrid Samsun X Argjiro had higher photosynthetic rates and lower transpiration rates in the early growth stage and the two rates were lower in the later stage, but it maintained higher photosynthetic rates for the whole growth stage. Net photosynthetic rates had a significant positive correlation with yield product, quality and resistance to black shank of the Oriental tobacco F1hybrids.

  7. Desempenho agronômico de híbridos F1 de tomate de mesa Agronomic attributes of F1 fresh market tomato hybrids

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    Fabrício Franco B dos Santos

    2011-09-01

    Full Text Available Objetivou-se avaliar e selecionar híbridos experimentais F1 de tomate de mesa do grupo salada quanto ao desempenho agronômico em condições de campo. Conduziu-se o experimento na Estação Experimental da Nunhems do Brasil, em Paulinia-SP, de fevereiro a junho de 2008. Obtiveram-se híbridos experimentais entre dez linhagens do BAG de tomate da Nunhems. Utilizou-se o delineamento experimental blocos casualizados com 36 tratamentos, quatro repetições e dez plantas por parcela. Com base no agrupamento de médias pelo teste de Scott-Knott, os resultados do desempenho agronômico dos híbridos experimentais para sete características avaliadas mostraram grande variabilidade entre os genótipos para comprimento de fruto e largura de fruto, com quatro grupos de médias. Os híbridos mostraram boa variabilidade para produção de frutos por planta e altura de planta, com três agrupamentos cada e, para as características número de frutos por planta, número de pencas por planta e distância da primeira penca do solo, mostraram-se menos divergentes, com dois grupos de médias. HE-38 e HE-14, com valores heteróticos positivos, destacaram-se como os mais produtivos em relação ao híbrido-padrão Aplauso.The study aims to obtain and select F1 resistant hybrids of tomato for fresh market and evaluate their agronomic attributes in field conditions. The work was carried out at Nunhems Experimental Station, located in Paulínia, São Paulo State, Brazil, from February to June 2008. The experimental design was completely randomized blocks with 36 treatments, four replications and ten plants per plot. Based on the grouping of means by Scott-Knott test, the performance of hybrids for seven agronomic traits showed great variability among genotypes for fruit length and width with four groups of means. There was good variability for yield per plant, and plant height with three groups of means for each character. The traits fruit number per plant, cluster

  8. Agronomic Performance of Flue-Cured Tobacco F1 Hybrids Obtained with Different Sources of Male Sterile Cytoplasm

    Directory of Open Access Journals (Sweden)

    Berbec A

    2014-12-01

    Full Text Available Four cytoplasmic male sterile (cms F1flue-cured hybrids cv. Wiaelica × cv. Virginia Golta (VG, the male fertile analogue and the parental varieties were tested at two locations in Poland in a replicated field trial. The cms sources in the hybrids wereN. suaveolens,N. amplexicaulis,N. bigeloviiand aN. tabacumcms mutant. Under the slight to moderate pressure from black root rot present at the trial sites the hybrids showed a moderate tolerance of the disease characteristic of VG as opposed to medium strong susceptibility of Wislica. Apart from the effect of black root rot tolerance the vegetative vigor of the hybrids (plant height, leaf size, earliness was affected by cytoplasm source. The F1hybrid withN. suaveolens cytoplasm flowered approximately three days later than the remaining hybrids. Of the cms hybrids tested cmsN. bigelovii produced the tallest plants with largest mid-position leaves. Yields of cured leaves were largely influenced by black root rot and were generally higher in VG and in the hybrids than in Wislica. Leaf yields and curability were generally little affected by cms source under low pressure from black root rot. At the site with a relatively high level of black root rot infestation the yields of cmsN. suaveolens were slightly lower but the percentage of light grades slightly higher compared to those of other cms hybrids. CmsN. suaveolens was the best hybrid in terms of money returns at the low black root rot field but it was the poorest hybrid performer under high pressure from the disease. Contents of nitrogen, sugars, nicotine and ash was little affected by source of cms. There was an increased incidence of potato virus Y (PVY and white spots in cmsN. suaveolens and, to a lesser extent, in cmsN. bigelovii as compared to the remaining disease-free entries.

  9. Expression of heterosis for productive traits in F1 eggplant (Solanum melongena L. hybrids

    Directory of Open Access Journals (Sweden)

    João Alencar de Sousa

    1998-03-01

    Full Text Available This study was carried out to obtain estimates of heterosis in crosses between seven eggplant cultivars (Embu = E; Santa Genebra = SG; Viserba = V; Aubergine de Barbentane = AB; Florida Market 10 = FM; Black Beauty = BB, and Melitino = M and two breeding lines (B-14-07 = B1 and B-31-06 = B2. The F1 hybrids used were: E x FM; E x BB; E x M; E x B1; E x B2; SG x FM; SG x BB; SG x M; SG x B1; SG x B2; V x FM; V x B1; V x B2; AB x FM; AB x M; AB x B1; AB x B2 and M x FM. Cultivars, lines and hybrids were evaluated at the ESAL experimental field in Lavras, MG, from February to October 1992. A randomized complete block design with three replications was used. Significant heterosis relative to the parental means was detected for all traits studied. Their values ranged from +41.23% to +113.31% for total fruit yield, from -11.45% to +26.17% for average fruit weight, and from +27.98% to +141.81% for early production. Heterosis relative to the superior parent ranged from +13.89% to +92.51% for total fruit yield. Hybrid pairs: SG x FM and AB x B1, V x FM and AB x FM, E x M and AB x B1 were the most heterotic relative to the parental mean for total fruit production, mean fruit weight and early production, respectively. The hybrids displaying highest heterosis relative to the superior parent for total yield were AB x B1 and SG x FM.Este trabalho visou obter estimativas da heterose em cruzamentos entre sete cultivares de berinjela (Embu = E, Santa Genebra = SG, Viserba = V, Aubergine de Barbentane = AB, Florida Market 10 = FM, Black Beauty = BB e Melitino = M e duas linhagens (B-14-07 = B1 e B-31-06 = B2. Os híbridos F1 utilizados foram: E x FM; E x BB; E x M; E x B1; E x B2; SG x FM; SG x BB; SG x M; SG x B1; SG x B2; V x FM; V x B1; V x B2; AB x FM; AB x M; AB x B1; AB x B2 e M x FM. O trabalho foi conduzido no campus da ESAL em Lavras-MG, de fevereiro a outubro de 1992. O delineamento empregado foi o de blocos casualizados completos com três repeti

  10. Two Tightly Linked Genes at the hsa1 Locus Cause Both F1 and F2 Hybrid Sterility in Rice.

    Science.gov (United States)

    Kubo, Takahiko; Takashi, Tomonori; Ashikari, Motoyuki; Yoshimura, Atsushi; Kurata, Nori

    2016-02-01

    Molecular mechanisms of hybrid breakdown associated with sterility (F2 sterility) are poorly understood as compared with those of F1 hybrid sterility. Previously, we characterized three unlinked epistatic loci, hybrid sterility-a1 (hsa1), hsa2, and hsa3, responsible for the F2 sterility in a cross between Oryza sativa ssp. indica and japonica. In this study, we identified that the hsa1 locus contains two interacting genes, HSA1a and HSA1b, within a 30-kb region. HSA1a-j (japonica allele) encodes a highly conserved plant-specific domain of unknown function protein (DUF1618), whereas the indica allele (HSA1a-i(s)) has two deletion mutations that cause disruption of domain structure. The second gene, HSA1b-i(s), encodes an uncharacterized protein with some similarity to a nucleotide-binding protein. Homozygous introgression of indica HSA1a-i(s)-HSA1b-i(s) alleles into japonica showed female gamete abortion at an early mitotic stage. The fact that the recombinant haplotype HSA1a-j-HSA1b-i(s) caused semi-sterility in the heterozygous state with the HSA1a-i(s)-HSA1b-i(s) haplotype suggests that variation in the hsa1 locus is a possible cause of the wide-spectrum sterility barriers seen in F1 hybrids and successive generations in rice. We propose a simple genetic model to explain how a single causal mechanism can drive both F1 and F2 hybrid sterility.

  11. Analysis of the meiosis in the F1 hybrids of Longiflorum x Asiatic(LA) of lilies (Lilium) using genomic in situ hybridization

    Institute of Scientific and Technical Information of China (English)

    Shujun Zhou; Munikote S. Ramanna; Richard G.F.Visser; Jaap M. van Tuyl

    2008-01-01

    Longiflorum and Asiatic lilies of the genus Lilium of the family Liliaeeae are two important groups of modern lily eultivars. One of the main trends of lily breeding is to realize introgression between these groups. With cut style pollination and embryo rescue, distant hybrids between the two groups have been obtained. However, the F1hybrids are highly sterile or some of them could produce a small number of 2n gametes, and their BC1 progenies are usually triploids. Dutch lily breeders have selected many cultivars from these BC1 progenies based on their variation. It is presumably suggested that such variation could be caused by intergenomic recombination and abnormal meiosis during gamete formation in F1 hybrids of Longiflorum x Asiatic (LA) hybrids in Lilium. Therefore, the meiotic process of ten F1 LA hybrids was cytologically investigated using genomic in situ hybridization and traditional cytological methods in the present research.The results showed that: at metaphase I, the homoeologous chromosome pairing among different F1hybrids ranged from 2.0 to 11.4 bi-valents formed by homoeologous chromosomes per pollen mother cell (PMC), and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all biva-lents were disjoined and most univalents were divided. Both the disjoined bivalents (half-bivalents) and the divided univalents (sister chromatids) moved to the opposite poles, and then formed two groups of chromosomes; because the two resulting half-bivalents retained their axes in the cell undisturbed, many crossover types, including single crossovers, three strand double crossovers, four strand double crossovers, four strand triple crossovers, and four strand multiple crossovers between the non-sister chromatids in the tetrads of bivalents,were clearly inferred by analyzing the breakpoints on the disjoined bivalents. The present investigation not only

  12. Complementation of the Fo c Subunit of Escherichia coli with That of Streptococcus mutans and Properties of the Hybrid FoF1 ATP Synthase

    OpenAIRE

    2013-01-01

    The c subunit of Streptococcus mutans ATP synthase (FoF1) is functionally exchangeable with that of Escherichia coli, since E. coli with a hybrid FoF1 is able to grow on minimum succinate medium through oxidative phosphorylation. E. coli F1 bound to the hybrid Fo with the S. mutans c subunit showed N,N′-dicyclohexylcarbodiimide-sensitive ATPase activity similar to that of E. coli FoF1. Thus, the S. mutans c subunit assembled into a functional Fo together with the E. coli a and b subu...

  13. Variation of Anthocyanin Composition in Fruit Skins of F_1 Hybrid Grapes

    OpenAIRE

    1992-01-01

    In order to know the heritability of B-ring modification of anthocyanins in grape skin, anthocyanin composition in F_1 genaration of grape cultivars were investigated ‘Muscat of Alexandria' which has green fruit color, had latent ability of methylation for B-ring of anthocyanin.‘Ioalia' was seemed to suit as parent for breeding of red grape cultivars, be cause of its low heritability of hydroxylation ability. The heritability of methylation ability in ‘Mills' showed lower than in ‘Rizamat' or...

  14. Effect of type and concentration of water hardness on the fertilization and hatching success of channel X blue F1 hybrid catfish eggs

    Science.gov (United States)

    Consistent and improved performance of channel x blue F1 hybrid catfish fingerlings in production ponds in the US farm raised catfish industry has prompted an increase in demand of hybrid catfish fingerlings even at higher prices compared to commonly raised channel catfish. Hybrid catfish fry are e...

  15. 高加索三叶草、白三叶及其杂种F1代的ISSR分析%ISSR Analysis of Trifolium ambiguum Bieb., T.repens L.and Their Hybrid F1

    Institute of Scientific and Technical Information of China (English)

    黄帆; 王明玖; 何丽君; 陈丽丽; 魏春秋

    2012-01-01

    Trifolium ambiguum Bieb. (Caucasian clover, paternal) is an important species for the traits such as resistance to drought and cold; T. repens L. (white clover, maternal) is a species introduced from Greater Khingan for the traits of strong nitrogen fixation ability and growing ability. Hybridization was made between Caucasian clover and white clover and the embryos were successfully raised using the tissue culture technique, then the regeneration system of F1 was established. In order to define the genetic difference degree between hybrids F1 and their parents, ISSR molecular makers were used to analyze the genetic relationship. The results showed that 170 bands of ISSR markers were amplified with 16 primers, and the length of DNA fragment were ranged from 100 to 2000bp; 138 bands were polymorphic, and the percentage of polymorphism bands reached 81. 18%. The genetic distance (GD) of 4 tested plant materials ranged from 0. 0756 to 0. 6798, with an average of 0. 5313. The genetic distance between Caucasian clover and white clover was larger; the hybrid F1 was closer to Caucasian clover than to white clover, and there was a slight variation between F1 hybrids and regeneration of F1. Authenticities of hybrid F1 were identified and genetic differences between hybrids and parents were analyzed from the level of molecular.%利用耐寒、耐旱的六倍体高加索三叶草与强固氮能力、生长能力的大兴安岭四倍体白三叶进行远缘杂交,通过胚拯救技术得到杂种F1,同时通过组培快繁技术建立了F1的再生体系.为了明确杂种F1与亲本在DNA水平上的差异,建立适用于F1的分子标记反应体系,利用ISSR技术对其进行了鉴定分析.结果表明,16个ISSR引物共扩增出170条条带,长度介于100~2000bp之间,多态性条带138条,多态性条带百分率为81.18%;4个供试材料间的遗传距离变幅为0.0756~0.6798,平均为0.5313;父本白三叶与母本高加索三叶草之间的遗传距离较大,F

  16. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 1

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2013-11-01

    Full Text Available An interspecific factorial hybridization was made, where seven P. strobus female parent trees were mated to four P. wallichiana male trees, in order to transfer genes for resistance to blister-rust from the blue pine to the F1 hybrids. The hybrid seedlings and the open pollinated families of the two parents were three times consecutivelyinoculated with blister-rust. The six years old seedlings were transplanted in the field in the so called Văliug Experiment 1. The experimental design consisted in 28 hybrid full-sib families and two open pollinated families of the parent species. Plantation took place in a complete randomized block design with three blocks, with 6 to 12 seedlings per linear plot and 3 x 3 m spacing. This paper is based on the measurements made at age 32, just after the first thinning. Diameter at breast height, tree height, tree volume, stem straightness and tree survival were the five measured traits. According to ANOVA, significant (p < 0.05 and highly significant (p< 0.001 differences for diameter at breast height, tree height, volume growth rate, stem straightness and tree survival were detected between hybrid families. Highly significant (p < 0.01, p < 0.001 differences were found between eastern white pine female parents for survival, stem straightness and all growth traits, suggestingthat nuclear additive genes are controlling the respective characters. Consequently, high survival or genetic resistance to blister-rust can be found within the P. strobus species even though it is highly susceptible to this pathogen. The P. wallichiana parents exhibited significant additive effects on straightness and breast height diameter, but no significance in height, volume, and tree survival. The contribution of the additive variance to the phenotypic one was 65.7% for diameter, 58.6% for height, 50.5% for volume, 70.4% for stem straightness and 73.4% for tree survival. Hence, additive variance should be employed in a breeding program

  17. InMotion hybrid racecar: F1 performance with LeMans endurance

    NARCIS (Netherlands)

    Jacob, J.; Colin, J.A.; Montemayor, H.; Sepac, D.; Trinh, H.D.; Voorderhake, S.F.; Zidkova, P.; Paulides, J.J.H.; Borisaljevic, A.; Lomonova, E.A.

    2015-01-01

    This paper presents the design of a hybrid electric powertrain for the InMotion IM01 race car. InMotion is a multidisciplinary project group of experienced master students, PhD students, and professors. The authors of this paper were involved in the project to develop a suitable powertrain

  18. InMotion hybrid racecar: F1 performance with LeMans endurance

    NARCIS (Netherlands)

    Jacob, J.; Colin, J.A.; Montemayor, H.; Sepac, D.; Trinh, H.D.; Voorderhake, S.F.; Zidkova, P.; Paulides, J.J.H.; Borisaljevic, A.; Lomonova, E.A.

    2015-01-01

    This paper presents the design of a hybrid electric powertrain for the InMotion IM01 race car. InMotion is a multidisciplinary project group of experienced master students, PhD students, and professors. The authors of this paper were involved in the project to develop a suitable powertrain architect

  19. Assessment of peroxidase isozyme marker-based model for cross identifications in hybrids (F(1)) of urdbean [ Vigna mungo (L.) Hepper].

    Science.gov (United States)

    Upadhyay, R.; Shukla, A.; Gaur, K.

    2002-12-01

    Four hybrids (4 F(1)s) were chosen out of crosses in the urdbean [ Vigna mungo (L.) Hepper, 2n = 22] having contrasting morphological characters. Zymograms for isozyme peroxidase were drawn from the patterns obtained from parents and their respective F(1) hybrids on the basis of relative similarities to parental bands. The selfed or crossed nature of hybrid pods was determined from the zymograms and their analysis. The number of bands and their intensities gave an idea about the extent of crossing in F(1) populations. Genetic identity (I) values were indicative of their selfed nature. Dendrograms were constructed on the basis of genetic identity values to display the relative similarities between the populations. Analysis was based on individual pods to confirm their hybrid or selfed nature. Possible use of this technique for identification of F(1) pods and elimination of selfed pods might be implemented to shorten the breeding operations during crossing.

  20. Effect of dietary administration of bananas on immunocytes in F1 hybrid calves.

    Science.gov (United States)

    Matsuda, Keiichi; Ohtsuka, Hiromichi; Ichijho, Toshihiro; Kawamura, Seiichi

    2006-01-01

    The effect of dietary administration of bananas on immunocytes in calves was investigated. Twenty Fl hybrid calves were used in this study (treated group n=10, control group n=10). Banana (2 g/kg BW) was administered to the calves for 5 days. Leukocyte subsets were examined on days 0, 5, 10, and 15. The numbers CD3+, (CD3+)CD45R-, and (CD3+)TcR+ cells significantly increased between day 0 and day 5 in the treated group (Pbanana to calves increased T-lymphocytes, suggesting it might be possible to enhance protective functions against infections.

  1. 江黄颡(Pelteobagrus vachelli)和乌苏里拟鳞(Pseudobagrus ussuriensis)杂交F1代形态差异%Morphometric differences of the hybrid F1 of Pelteobagrus vachelli × Pseudobagrus ussuriensis

    Institute of Scientific and Technical Information of China (English)

    蔡永祥; 陈友明; 陈校辉; 王明华; 潘莹; 夏爱军

    2011-01-01

    通过测定江黄颡雌、雄亲本,乌苏里拟鲿雌、雄亲本以及它们的正交F1代和反交F1代共6个实验鱼组合的形态和框架数据,运用卡方分析和多无分析方法,比较了杂交F1代与亲本之间的形态异同.可数性状卡方分析结果表明正、反交F1代与双亲在大部分可数性状上是一致的.但在胸鳍鳍条数上存在明显差异,胸鳍鳍条数可以作为区别杂交F1代与双亲的重要参数,在臀鳍和尾鳍性状上.杂交F1代与江黄颡亲本差异明显,而与乌苏里拟鲿亲本无差异.形态和框架数据的聚类分析结果表明,两亲本雌鱼组合与雄鱼组合之间、正交F1与反交F1实验鱼组合之间的形态最为接近,但正、反杂交F1代较其亲本性状出现了一定程度的多样化;主成分分析结果表明,6个组合的实验鱼在形态上的差别主要由鱼体体高的长度差异引起;以判别分析方法构建了 6个判别方程,其综合判别率为97.9%.三种多元分析结果表明,正、反杂交F1代较其亲本形态产生了一定程度的差异,正、反杂交子代在形态上较接近于乌苏里拟鲿.%The morphological variations of the reciprocal F1 hybrids between Pelteobagrus vachelli and Pseudobagrus ussuriensis were analyzed by Chi-square analysis and multivariate data analysis methods based on the parameters of morphometric characters. The results of Chi-square analysis indicated that there were no significant differences between the reciprocal F1 hybrids and their parents,except for the number of pectoral fin. The number of pectoral fin can be used as an important parameter to discriminate the reciprocal F1 hybrids from their parents. In aspect of the characters of anal fin and caudal fin, there were significant differences between the reciprocal F1 hybrids and their parents of P. vachelli, but not significant between reciprocal F1 hybrids and their parents of P. ussuriensis. The results of cluster analysis have revealed that the

  2. Analysis of F1 hybrid and BC1 monosomic alien addition line plants from Brassica oleracea × Sinapis alba by GISH

    Institute of Scientific and Technical Information of China (English)

    WEI Wenhui; ZHANG Sufeng; LI Jun; WANG Lijun; CHEN Bo; FANG Xiaoping; WANG Zhuan; LUO Lixia

    2006-01-01

    Sterile and semi-fertile F1 plants were obtained by intergeneric sexual hybridization between paternal Brassica oleracea var. alboglabra (genome CC, 2n=18) and maternal Sinapis alba (genome SS, 2n=24), BC1 plants were obtained by backcrossing between paternal B. oleracea and maternal semi-fertile F1 plants. Genomic in situ hybridization (GISH) combined with dual-colour fluorescence in situ hybridization (dcFISH) showed that sterile F1 plants contained 21 chromosomes consisting of one B. oleracea chromosome set and one S.alba chromosome set, belonging to expected hybrids,and semi-fertile F1 plants contained 30 chromosomes consisting of two B. oleracea chromosome sets and one S. alba chromosome set. It is obvious that the semi-fertile F1 plants belong to unexpected hybrids.1 -3 trivalents were detected at meiotic metaphase I of semi-fertile F1 pollen mother cells (PMCs). Different separation ratios of S chromosomes were detected at anaphase I. A monosomic alien addition line(MAAL) was identified by GISH-dcFISH from BC1plants; it contained 19 chromosomes consisting of 18 C chromosomes and 1 S chromosome. At meiotic metaphase I, 9 divalents from B. oleracea and one univalent from S. alba could be detected. Sometimes,one putative C-S trivalent could also be detected.The achievement of B. oleracea-S, alba monosomic alien addition lines lays a foundation for gene introgression, location and cloning.

  3. Maternal Effects on Seed and Seedling Phenotypes in Reciprocal F1 Hybrids of the Common Bean (Phaseolus vulgaris L.)

    Science.gov (United States)

    Singh, Jugpreet; Clavijo Michelangeli, Jose A.; Gezan, Salvador A.; Lee, Hyungwon; Vallejos, C. Eduardo

    2017-01-01

    Maternal control of seed size in the common bean provides an opportunity to study genotype-independent seed weight effects on early seedling growth and development. We set out to test the hypothesis that the early heterotrophic growth of bean seedlings is determined by both the relative amount of cotyledon storage reserves and the genotype of the seedling, provided the hybrid genotype could be fully expressed in the seedlings. The hypothesis was tested via comparison of seed weight and seedling growth phenotypes of small-seeded (wild, ~0.10 g) and large-seeded (landrace, ~0.55 g) parents and their reciprocal F1 hybrids. Akaike's Information Criteria were used to estimate growth parameters and identify the phenotypic model that best represented the data. The analysis presented here indicates that the hybrid embryo genotype is not fully expressed during both seed and seedling growth and development. The analysis presented here shows that seed growth and development are controlled by the sporophyte. The strong similarity in seed size and shape of the reciprocal hybrid seed with seeds of the maternal parents is evidence of this control. The analysis also indicates that since the maternal sporophyte controls seed size and therefore the amount of cotyledon reserves, the maternal sporophyte indirectly controls early seedling growth because the cotyledons are the primary nutrient source during heterotrophic growth. The most interesting and surprising results indicated that the maternal effects extended to the root architecture of the reciprocal hybrid seedlings. This phenomenon could not be explained by seed size, but by alterations in the control of the pattern of gene expression of the seedling, which apparently was set by a maternally controlled mechanism. Although seed weight increase was the main target of bean domestication, it also had positive repercussions on early-growth traits and stand establishment. PMID:28174586

  4. 小麦杂交F1和双亲幼苗动态蛋白质组分析%Dynamic Changes of Leaves Proteome Between Wheat F1-hybrid and Its Parental Lines

    Institute of Scientific and Technical Information of China (English)

    甘富; 段爽; 彭永康

    2012-01-01

    In this study,proteome approaches were applied to a comparative analysis of hybridization in wheat F,-hybrid and its parent lines by using 2,3,5 d leaves. The results indicated that protein expression differences were observed between F, -hybrid and parents. In the 5 d leaves ,27 protein spots were changed in the protein abundances and only 15 protein spots were changed in the protein abundances at 2 d,3 d leaves in F, hybrid. In these changed proteins, most protein spots were showed "two parental lines" characteration ( 41% ) and "low parents" charactera-tion(44% ) in protein abundances, we also observed the some protein sports were lost and some new protein spots were induced in the F, -hybrid. These results indicated that hybridization between two parental lines could cause expression differences between wheat hybrid and its parents at protein abundances.%分别以2,3,5d叶龄的栽培小麦京411、偏硬001和杂交F1幼苗为材料,利用动态蛋白质组技术,对F1及双亲的蛋白质变化做了比较分析,结果表明,在双亲及F1中蛋白质差异表达现象被观察到,其中在5d的叶子中最为明显.如在发育2d和3d的F1叶子中,各有15个蛋白质斑点的含量产生变化,而在5d叶龄的叶子中,这种有变化的蛋白质斑点达27个,在这些变化的蛋白质中,偏双亲和低于双亲的为多数,如5d的叶子中分别占41%和44%,同时,在F1叶片中观察到有蛋白质消失和新的蛋白质诱导现象产生.这些结果表明,杂种中存在蛋白质的差异表达现象,展开这方面的研究有助于对杂种优势形成机理的理解.

  5. GISH Analysis of Subsequent Progeny Crossed with 2n-gametes of F-1 Oriental-Asiatic Interspecific Hybrid in Lily

    NARCIS (Netherlands)

    Chung, M.Y.; Chung, J.D.; Tuyl, van J.M.; Lim, K.B.

    2009-01-01

    This study was carried out to analyze the chromosome composition and homoeologous recombination of subsequent progenies, derived from cross of Oriental-Asiatic (OA) interspecific hybrid producing 2n-gametes, by GISH technique. GISH analysis of F-1 OA interspecific hybrid '2nOA-001', which produced 2

  6. Micropropagation of an elite F1 watermelon (Citrullus lanatus hybrid from the shoot tip of field grown plants

    Directory of Open Access Journals (Sweden)

    Mohammad Khalekuzzaman

    2012-06-01

    Full Text Available The aim of this work was to develop a protocol for rapid micropropagation of an elite F1 hybrid watermelon cultivar using shoot tip of field-grown plants. Maximum frequency (73% of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA and 0.1 mg l-1 indole-3 acetic acid (IAA. Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots and 2.0 mg l-1 BA was optimum in this respect. Addition of gibberellic acid (GA3 in the multiplication medium resulted in better growth of shoots. Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA. The shoots produced more roots with increasing number of subcultures. About 72% of the regenerated plantlets acclimatized successfully and survived in the soil condition.

  7. Hybridization between Helicoverpa armigera and Helicoverpa assulta (Lepidoptera: Noctuidae): development and morphological characterization of F1 hybrids

    NARCIS (Netherlands)

    Zhao, X.C.; Dong, J.F.; Tang, Q.B.; Yan, Q.B.; Celbic, I.; Loon, van J.J.A.; Wang, C.Z.

    2005-01-01

    Reciprocal hybridizations between Helicoverpa armigera (Hubner) and Helicoverpa assulta (Guenee) were studied. The cross between females of H. armigera and males of H. assulta yielded only fertile males and sterile individuals lacking an aedeagus, valva or ostium bursae. A total of 492 larvae of the

  8. Hybridization between Helicoverpa armigera and Helicoverpa assulta (Lepidoptera: Noctuidae): development and morphological characterization of F1 hybrids

    NARCIS (Netherlands)

    Zhao, X.C.; Dong, J.F.; Tang, Q.B.; Yan, Q.B.; Celbic, I.; Loon, van J.J.A.; Wang, C.Z.

    2005-01-01

    Reciprocal hybridizations between Helicoverpa armigera (Hubner) and Helicoverpa assulta (Guenee) were studied. The cross between females of H. armigera and males of H. assulta yielded only fertile males and sterile individuals lacking an aedeagus, valva or ostium bursae. A total of 492 larvae of the

  9. Genetic Analysis of Heterosis for Number of Spikelets per Panicle and Panicle Length of F1 Hybrids in japonica Rice Hybrids

    Institute of Scientific and Technical Information of China (English)

    ZHU Xu-dong; HONG De-Lin; SHEN Ying; LENG Yan; Henri ADREIT; Julien FROUIN; Didier HARREAU

    2004-01-01

    Genetic analysis of heterosis for number of spikelets per panicle and panicle length of F1 hybrid was conducted by using japonica rice varieties Bing 8979, C Bao, their F1, F2 and triple test cross (TTC) progenies. The two traits, panicle length and number of spikelets per panicle were controlled by polygenes, which were dispersed in the two parents. The dispersion of these polygenes was the genetic basis for the heterosis. Genetic variation in panicle length was mainly due to additive and dominant effects, and the dominant component played a determinative role. For number of spikelets per panicle, the effect of non-allelic genes was highly significant (1% probability level), and there existed epistasis including effects of additive × additive, additive×dominant, and dominant × dominant.

  10. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 1

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2013-12-01

    Full Text Available An interspecific factorial hybridization was made, where seven P. strobus female parent trees were mated to four P. wallichiana male trees, in order to transfer genes for resistance to blister-rust from the blue pine to the F1 hybrids. The hybrid seedlings and the open pollinated families of the two parents were three times consecutively inoculated with blister-rust. The six years old seedlings were transplanted in the fi eld in the so called Valiug Experiment 1. The experimental design consisted in 28 hybrid full-sib families and two open pollinated families of the parent species. Plantation took place in a complete randomized block design with three blocks, with 6 to 12 seedlings per linear plot and 3 x 3 m spacing. This paper is based on the measurements made at age 32, just after the fi rst thinning. Diameter at breast height, tree height, tree volume, stem straightness and tree survival were the fi ve measured traits. According to ANOVA, signifi cant (p < 0.05 and highly signifi cant (p < 0.001 differences for diameter at breast height, tree height, volume growth rate, stem straightness and tree survival were detected between hybrid families. Highly signifi cant (p< 0.01, p < 0.001 differences were found between eastern white pine female parents for survival, stem straightness and all growth traits, suggesting that nuclear additive genes are controlling the respective characters. Consequently, high survival or genetic resistance to blister-rust can be found within the P. strobus species even though it is highly susceptible to this pathogen. The P. wallichiana parents exhibited signifi cant additive effects on straightness and breast height diameter, but no signifi cance in height, volume, and tree survival. The contribution of the additive variance to the phenotypic one was 65.7% for diameter, 58.6% for height, 50.5% for volume, 70.4% for stem straightness and 73.4% for tree survival. Hence, additive variance should be employed

  11. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 1

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2013-12-01

    Full Text Available An interspecific factorial hybridization was made, where seven P. strobus female parent trees were mated to four P. wallichiana male trees, in order to transfer genes for resistance to blister-rust from the blue pine to the F1 hybrids. The hybrid seedlings and the open pollinated families of the two parents were three times consecutively inoculated with blister-rust. The six years old seedlings were transplanted in the fi eld in the so called Valiug Experiment 1. The experimental design consisted in 28 hybrid full-sib families and two open pollinated families of the parent species. Plantation took place in a complete randomized block design with three blocks, with 6 to 12 seedlings per linear plot and 3 x 3 m spacing. This paper is based on the measurements made at age 32, just after the fi rst thinning. Diameter at breast height, tree height, tree volume, stem straightness and tree survival were the fi ve measured traits. According to ANOVA, signifi cant (p < 0.05 and highly signifi cant (p < 0.001 differences for diameter at breast height, tree height, volume growth rate, stem straightness and tree survival were detected between hybrid families. Highly signifi cant (p< 0.01, p < 0.001 differences were found between eastern white pine female parents for survival, stem straightness and all growth traits, suggesting that nuclear additive genes are controlling the respective characters. Consequently, high survival or genetic resistance to blister-rust can be found within the P. strobus species even though it is highly susceptible to this pathogen. The P. wallichiana parents exhibited signifi cant additive effects on straightness and breast height diameter, but no signifi cance in height, volume, and tree survival. The contribution of the additive variance to the phenotypic one was 65.7% for diameter, 58.6% for height, 50.5% for volume, 70.4% for stem straightness and 73.4% for tree survival. Hence, additive variance should be employed

  12. Genetic Phenotype of Catechins in Tea Plants Hybrid F1 Generation%茶树杂交F1代儿茶素类的遗传表型研究

    Institute of Scientific and Technical Information of China (English)

    涂良剑; 郑金贵; 林金科; 杨志坚; 林用松; 黄则栋; 赵文净; 孙平; 严婷婷

    2012-01-01

    以高EGCG茶树新品系为主要杂交亲本,分析其杂交F1代儿茶素类的遗传表型.结果表明:杂交F1代的EGC、EC、EGCG、ECG、酯型儿茶素类、简单儿茶素类、TC(儿茶素总量)、酯型儿茶素类/TC、简单儿茶素类/TC、EGCG/咖啡碱、EGCG/TC、儿茶素(类)品质指数等指标,不同株系差异很大;不同杂交组合存在显著差异,Kruskal Wallis检验和稳健检验均达显著性水平;不同杂交组合的分离存在极显著性差异,方差齐性检验表明达到极显著性水平.不同杂交组合F1代TC的分布因亲本的遗传力而定;不同杂交组合F1代高EGCG单株、高酯型儿茶素类单株和高儿茶素(类)品质指数单株的分布也因亲本的遗传力而定.%The hybrid F1 generation of the new tea plants with high EGCG content were used in this experimentation to study the genetic phenotype of catechins. The results showed that (a) the contents of EGC, EC, EGCG, ECG, ester-catechins, simple catechins and TC (total catechins), the ratios of ester-catechins/TC, simple catechins/TC, EGCG/caffeine and EGCG/TC, as well as the catechin quality index of the tea plants differed significantly among different varieties; (b) the differences among the hybrid combinations reached a significant level according to Kruskal Wallis and the robust tests; and (c) the different hybrid combinations separated very significantly from one another based on the homogeneity of variance test. The TC distribution in the F1 generation of the hybrid combinations was determined by the genetic heritability. The distribution of individual plants with high EGCG, high ester-catechins and high quality catechins index was also determined by the heritability of their parents' genetic.

  13. Comparisons of Photosynthetic Characteristics in Relation to Lint Yield Among F1 Hybrids, Their F2 Descendants and Parental Lines of Cotton

    Institute of Scientific and Technical Information of China (English)

    FENG Guo-yi; GAN Xiu-xia; YAO Yan-di; LUO Hong-hai; ZHANG Ya-li; ZHANG Wang-feng

    2014-01-01

    We compared the photosynthetic characteristics in relation to yield of two F1 cotton hybrids (Shiza 2-F1 and Xinluzao 43-F1), their parental lines (NT2, H2 and 4-14) and their F2 descendants at different growth and development stages. The two F1 exhibited heterobeltiosis in net photosynthetic rate (Pn) by 8.1-52.1%, canopy apparent photosynthetic rate (CAP) by 8.2-57.6% and canopy respiration rate (CR) by 3.0-78.7% during the growing season. They also exhibited mid-parent heterosis by 2.0-5.2% in leaf chlorophyll content (SPAD) during the late growth and development stages. Regression analysis showed that both parents contributed to increase in Pn, SPAD and CAP in the F1. A low CR in the F1 matched a low CR of the parental line. Photosynthetic characteristics in the F2 were mainly dependent upon the magnitude and degeneration rate of the F1. Mid-parent heterosis in CAP and in CR during the late growth and development stage reduced the degeneration of the F2. Average dry matter accumulation was 10.7-34.7% higher in the parental lines of Xinluzao 43-F1 than in the parental lines of Shiza 2-F1. Heterobeltiosis in dry matter accumulation was 7.0-23.1% greater for Xinluzao 43-F1 than for Shiza 2-F1. Dry matter accumulation in the F1 was affected by either the dry matter accumulation of parents or heterobeltiosis. Dry matter accumulation in the F2 was mainly inlfuenced by dry matter accumulation in the F1. The yields of the two F1 were 39.1-46.3% higher than their respective parents and 26.4-45.9% higher than that of the conventional cultivar Xinluzao 33. The yields of the two F2 were 9.2-12.8% higher than the parents and 14.9-27.4% higher than that of Xinluzao 33. The photosynthetic production and yield of the F1 and F2were higher than that of their parents. The increases in Pn and CAP of the F1 and F2 were dependent on the photosynthetic characteristics of their parents. It is thus concluded that the photosynthetic performance, light use efifciency and yield of the F1

  14. Qualidade de frutos de tomate da cv. Santa Clara, mutante de fruto amarelo e seus híbridos F1 Quality of tomato red fruit, cv. Santa Clara, its yellow mutant and respective F1 hybrid

    Directory of Open Access Journals (Sweden)

    Elizanilda R. do Rêgo

    1999-07-01

    Full Text Available A caracterização de alguns atributos físicos e químicos de frutos de tomate da cv. Santa Clara, um mutante de fruto amarelo, e o híbrido F1 obtido do cruzamento entre eles, foi feita com base nos teores de b-caroteno, licopeno, vitamina C, pH, matéria fresca, matéria seca, diâmetro do fruto, diâmetro do pericarpo, acidez e sólidos solúveis totais dos tecidos loculares e do pericarpo. Os frutos maduros amarelos apresentaram teores reduzidos de b-caroteno, licopeno e vitamina C, enquanto o híbrido apresentou teores semelhantes ao genótipo normal. Tanto o fruto mutante quanto o F1 apresentaram teor de pH menor que o do fruto normal. Não houve diferença significativa quanto a matéria fresca e seca totais, diâmetro do fruto e do pericarpo e acidez. Os teores de sólidos solúveis totais do pericarpo foram inferiores nos frutos do mutante amarelo e F1, em relação ao genótipo normal, enquanto os teores de sólidos solúveis totais da massa locular não diferiram, estatisticamente, entre os genótipos.The determination of some physical and chemical attributes of red fruits from tomato cv. Santa Clara, a yellow mutant and the F1 hybrid, were based on b-carotene, lycopene, vitamin C, pH, fresh and dry weight matter, pericarp and fruit diameter, titrateable acidity and total soluble solids. Ripe fruits of the yellow mutant showed reduced levels of b-carotene, lycopene and vitamin C, while fruits of the F1 hybrid showed levels of carotenoid pigments and vitamin C similar to the red fruit. Both mutant and F1 ripe fruits showed lower pH levels than the red parentals. Total fresh fruit and dry weight matter, pericarp and fruit diameter, and acidity were similar in the three genotypes studied. The total soluble-solids content of the pericarp was lower in the mutant and F1 fruits, whereas their concentration in the locule tissues was similar in all genotypes.

  15. 育肥性能分析大凉山黑猪杂交F1代%Fattening Performance Analysis of Daliangshan Black Pig′s Hybridization F1

    Institute of Scientific and Technical Information of China (English)

    黄剑锋; 黄卫平

    2016-01-01

    大凉山黑猪生长速度慢,用杜洛克、巴克夏等优质公猪杂交改良后,育肥性能有了明显提高。采用生长曲线拟合与回归分析可以科学估算大凉山黑猪及其F1代的出栏时间,其中纯种黑猪的拐点体重为71.6 kg、出栏时间为193.4 d,杜杂黑猪为90.3 kg、200.4 d,巴杂黑猪为89.7 kg、198.6 d。%The growth speed of Daliangshan black pigs was slow, its fattening performance was improved obviously by crossbreeding with Duroc boar and Berkshire boar. Using growth curve fitting with the regression analysis could estimate the marketing time and spinodal weight, the pure black pig was 71.6 kg and 193.4 days, Duroc hybridization F1 was 90.3 kg and 200.4 days, Berkshire hybridization F1 was 89.7 kg and 198.6 days.

  16. Cytological Analyses on Development of Male and Female Gametophytes in an Interspecific Hybrid F1 from Cucumis hystrix Chakr. × Cucumis sativus L.

    Institute of Scientific and Technical Information of China (English)

    LUO Xiang-dong; DAI Liang-fang; QIAN Chun-tao; CHEN Jin-feng

    2006-01-01

    An interspecific hybrid F1 of Cucumis hystrix Chakr. × Cucumis sativus L. (NC4406) was used to establish the developmental sequence and to characterize the male and female gametophytes at cytological level for further understanding of the phylogenic relationship and the mechanism of fertility or sterility in the interspecific hybrid F1. The development of male and female gametophytes was studied through meiotic analysis and paraffin section observation technique, respectively.Meanwhile, the fertility level was assessed through hybrid F1 backcrossing to cultivated cucumber 4406. Variable chromosome confgurations were observed in the pollen mother cells (PMCs) of hybrid F1 at metaphase Ⅰ, e.g., univalents,bivalents, trivalents, quadravalents, etc. At anaphase Ⅰ and Ⅱ, chromosome lagging and bridges were frequently observed as well, which led to the formation of polyads and only a partial number of microspores could develop into fertile pollen grains (about 23.3%). Observations of the paraffin sections showed numerous degenerated and abnormal embryo sacs during the development of female gametophytes, and only 40% of the female gametophytes could develop into normal eight-nuclear megaspore. On an average, 22.8 and 6.3 seeds per fruit could be obtained from the reciprocal backcross. The interspecific hybrid F1 of C. hystrix × NC4406 was partially fertile; however, the meiotic behaviors of hybrid F1 showed a high level of intergenomic recombination between C. hystrix and C. sativus chromosomes, which indicated that it plays an important role for introgression of useful traits from C. hystrix into C. sativus.

  17. F1 hybrids of BALB/c and C57BL/6 mouse strains respond differently to low-dose ionizing radiation exposure

    Indian Academy of Sciences (India)

    Sanjay Mukherjee; K. B. Sainis; Deepti D. Deobagkar

    2014-12-01

    There are evidences to show that response to ionizing radiations have genetic influence. To investigate this further, reciprocal F1 hybrids were genereted by crossbreeding the radiation-susceptible BALB/c mouse strain with resistant C57BL/6 in a sex-specific manner (BALB/c♂×C57BL/6♀ = B6BcF1; C57BL/6♂× BALB/c♀ =BcB6F1). These hybrids were compared with each other and to the parental strains with respect to transcriptional responses to low-dose ionizing radiation exposure (LDIR). The two F1 hybrids showed drastic differences in their gene expression profiles to ionizing radiation exposure particularly in case of the genes involved in DNA damage response and repair process. Also, the inheritance pattern of the gene expression was found to be complex and could not be explained solely on the basis of parental expression pattern. It was concluded that there is a differential transmission of susceptible trait alleles from the parents to F1 progeny which is dependent on the sex of the parent mouse strain used to set up the crosses and other environmental factors.

  18. Complementation of the Fo c subunit of Escherichia coli with that of Streptococcus mutans and properties of the hybrid FoF1 ATP synthase.

    Science.gov (United States)

    Araki, Makoto; Hoshi, Kazuya; Fujiwara, Masasuke; Sasaki, Yuka; Yonezawa, Hideo; Senpuku, Hidenobu; Iwamoto-Kihara, Atsuko; Maeda, Masatomo

    2013-11-01

    The c subunit of Streptococcus mutans ATP synthase (FoF1) is functionally exchangeable with that of Escherichia coli, since E. coli with a hybrid FoF1 is able to grow on minimum succinate medium through oxidative phosphorylation. E. coli F1 bound to the hybrid Fo with the S. mutans c subunit showed N,N'-dicyclohexylcarbodiimide-sensitive ATPase activity similar to that of E. coli FoF1. Thus, the S. mutans c subunit assembled into a functional Fo together with the E. coli a and b subunits, forming a normal F1 binding site. Although the H(+) pathway should be functional, as was suggested by the growth on minimum succinate medium, ATP-driven H(+) transport could not be detected with inverted membrane vesicles in vitro. This observation is partly explained by the presence of an acidic residue (Glu-20) in the first transmembrane helix of the S. mutans c subunit, since the site-directed mutant carrying Gln-20 partly recovered the ATP-driven H(+) transport. Since S. mutans is recognized to be a primary etiological agent of human dental caries and is one cause of bacterial endocarditis, our system that expresses hybrid Fo with the S. mutans c subunit would be helpful to find antibiotics and chemicals specifically directed to S. mutans.

  19. Genetic improvement on Chinese shrimp ( Fenneropenaeus chinensis): growth and viability performance in F1 hybrids of different populations

    Science.gov (United States)

    Tian, Yi; Kong, Jie; Li, Wendong; Luan, Sheng; Yang, Cuihua; Wang, Qingyin

    2008-11-01

    Fenneropenaeus chinensis distributed in the Yellow Sea and Bohai Sea of China and the west coast of the Korean Peninsula. Different geographical populations represent potentially different genetic resources. To learn further the characteristics of different geographical population, crosses among two wild and three farmed populations were produced. The two wild populations were from the Yellow Sea and Bohai Sea (WYP), and the west coast of the Korean Peninsula and coast (WKN). The three farmed populations included the offspring of first generation of wild shrimp from coast in Korea (FKN), the Huang Hai (the Yellow Sea in Chinese) No.1 (HH1), and JK98. The phenotypes growth and survival rates of these populations were compared to confirm the feasibility for crossbreeding. The body length (BL), carapace length (CL), carapace width (CW), height of the second and third abdominal segment (HST), width of the second and third abdominal segment (WST), length of the first abdominal segment (LF), length of the last abdominal segment (LL), live body weight (BW), and survival rate were measured. Different combinations were statistically performed with ANOVA and Duncan’s Multiple Range Test. The results show that the survival rate of JK98(♀)×WKN(♂) was the highest, followed by WYP(♀)×WKN(♂), FKN(♀)×WYP(♂), FKN(♀)×HH1(♂) and WYP(♀)×FKN(♂); the body weight of FKN(♀)sxHH1(♂) was the highest, followed by FKN(♀)×WYP(♂), WYP(♀)×WKN(♂), WYP(♀)×FKN(♂) and JK98(♀)×WKN(♂); the total length had the same ranking as the body weight. All growth traits in hybrids JK98(♀)×WKN(♂) were the lowest among all combinations. F1 hybrids had significant difference (P0.05) in other growth traits and survival rate. The results of Duncan’s Multiple Range Test are that BL and CL of JK98(♀)×WKN(♂) were significantly different from the other combinations; HST different from the combination of FKN(♀)×WYP(♂), FKN(♀)×HH1(♂) and WYP

  20. Occurrence of 2n gametes in the F1 hybrids of Oriental x Asiatic lilies (Lilium): Relevance to intergenomic recombination and backcrossing

    NARCIS (Netherlands)

    Barba Gonzalez, R.; Lim, K.B.; Ramanna, M.S.; Visser, R.G.F.; Tuyl, van J.M.

    2005-01-01

    Cytological modes of the origin of 2n gametes were investigated in six different genotypes of F1 hybrids between Oriental and Asiatic (OA) lilies (Lilium, 2n = 2x = 24). Chromosome pairing between the parental genomes was very low, the average frequency range from 0.3 to 1.2 bivalents per cell among

  1. Studies on Morphology of F1 Hybrid and Systematic Evolution of Epimedium Species%淫羊藿属植物杂交F1代植株形态和种间系统演化研究

    Institute of Scientific and Technical Information of China (English)

    杜明凤; 陈庆富

    2013-01-01

    淫羊藿属植物多数种类在形态分类性状上存在连续过渡的形态变异现象,导致种间的鉴别困难.本研究对粗毛淫羊藿、巫山淫羊藿、天平淫羊藿,3个物种合计29个居群之间的种间杂交F1代植株及其亲本形态学进行了比较研究.结果表明:AC×MY、AC×WU、WU×MY 3种组合杂交F1代的叶片形状表现为双亲过渡形态,控制淫羊藿花瓣距短于内萼片的基因可能是部分显性,E.acuminatum的花瓣和内萼片颜色属于显性遗传.所得结果为淫羊藿属植物分类学、种间系统关系及其起源与进化研究提供了新的资料和线索.%It is known that there are continuous variations of morphological characteristics for taxonomy among most Epimedium species,leading to difficulties of species identification.The morphology of interspecific F1 hybrid plants among 29 populations of E.acuminatum,E.wushanese and E.myrianthum was studied comparatively.The results shows that the length of flower calcar shorter than the inner sepal may be controlled by partly-dominant gene,and the leaf shape of hybrid F1 progenies are the middle type between the parents.The colour of the inner sepal and flower calcar in E.acuminatum is controlled by dominant genes.These above results are useful on interspecific hybridization and variety improvement on Epimedium.

  2. Production of intraspecific F1 hybrids between wild and cultivated accessions of cowpea (Vigna unguiculata (L.) walp.) using conventional methods.

    Science.gov (United States)

    Lelou, B; Van Damme, P

    2006-01-01

    Cowpea (Vigna unguiculata (L.) Walp.) is an important food legume in the tropics. It belongs to the Phaseoleae (L.) tribe (Fabaceae family), it is diploid and its chromosome number is 22. Its gene pool includes the cultivated cowpea and its wild relatives, which are connected with Vigna subgenus, Catiang section. Cowpea has a great potential in increasing food legume production. The cowpea varieties, however, are susceptible to a number of insect pests, especially the pod borer Maruca testulalis and a pod sucking-bug complex (e.g.: Clavigralla tomentosicollis, Anoplocnemis curvipes and Riptortus dentipes), which cause severe damage. The crossing programme presented here exploits the variability existing in the wild African germplasm of V. unguiculata and cultivated cowpea. To incorporate the insect pest resistance into the cultivated cowpea economically, reciprocal crosses between wild forms and cowpea varieties were performed, using the stigmatic pollination methods at anthesis. Some barriers were found in these intraspecific crosses. In the majority of reciprocal crosses, the growth of the pollen tubes was arrested in the stigmatic tissue. Only 16.01% of the ovules were fertilised. In these ovules, embryo development was normal at about 20-25 days after pollination. The failure of the intraspecific crosses in about 80.7% of the cases is thus the result of the lack of fertilisation and the unfertilised ovules. There seems to exist considerable incompatibility within the primary cowpea gene pool. The breeding programme carried out under controlled conditions has proved to be less successful in developed cowpea intraspecific F1 hybrids. Further studies should concentrate on germplasm from Africa with documented resistance to major insect pests. In addition, the application of techniques for bypassing barriers to hybridisation of parent genotypes should enable these embryos to grow to plants.

  3. A New Chinese Kale F1Hybrid-‘Jinlyu’%芥蓝新品种金绿的选育

    Institute of Scientific and Technical Information of China (English)

    李向阳; 刘振翔; 刘自珠; 郑岩松; 张华; 邹集文; 李光光; 黄红弟

    2014-01-01

    金绿是利用雄性不育系S6-3-2A为母本,自交系G028-3-3为父本配制成的芥蓝一代杂种,早中熟,播种至初收61 d(天),长势强,植株健壮,直立较紧凑;叶片卵圆形,深绿色,叶柄较短,薹叶较小,薹色绿,菜薹紧实匀条,抽薹整齐,花球大,齐口花,质爽脆味甜,纤维少,商品综合性状好,品质优。主薹高23.5 cm,横径2.3 cm,质量165 g。抗逆性强,对霜霉病和软腐病的抗性比对照绿宝强。每667 m2产量为1 030~2 000 kg。适宜全国各地种植。%‘Jinlyu’ is a Chinese kale F1 hybrid developed by crossing male sterile line S6-3-2A as female parent,and inbred line G028-3-3 as male parent. ‘Jinlyu’ is an early-medium maturing cultivar. It takes 61 days from sowing to first harvest. The plant has strong growth vigor,erect and compact plant type. Its leaf blades are of oval round shape with dark green color. The leaf stalk is short. Its head is large in size. It tastes crspy and sweet with less fiber. It has good commercial characteristics and superior guality. Its major sprout is 23.5 cm in height,2.3 cm in diameter,and about 165.0 g in weight. ‘Jinlyu’ grows vigorously and is highly resistant to stress. Its resistance to downy mildeu and soft rot is better than the contrast ‘Lyubao’. It can yield 15.5-30.0 t·hm-2. It is suitable to be planted all over the China.

  4. Temporal and Spatial Expression of Bt Toxin in Transgenic Restorer Line and Its F1 Hybrids%转基因水稻恢复系及其F1代Bt蛋白的时空表达分析

    Institute of Scientific and Technical Information of China (English)

    汪秀峰; 叶芬; 李莉; 陆徐忠; 倪大虎; 王德正; 麦霄黎; 王淑云; 杨剑波

    2014-01-01

    本研究以抗虫水稻恢复系9311(Bt)及其杂交种F1(Bt)为研究材料,以非转基因的9311为阴性对照,利用ELISA方法研究9311(Bt)及F1(Bt)各生育时期可溶性总蛋白和Bt蛋白的时空变化规律,为转Bt基因抗虫水稻的安全监管提供科学依据。结果表明:外源基因的导入没有引起水稻组织中可溶性总蛋白含量的明显变化;9311(Bt)的Bt蛋白表达量在整个生长周期的各个部位均高于相应的F1(Bt)植株;同一植株不同组织器官中Bt蛋白表达量为:叶片跃胚乳跃颖壳及茎秆跃根;同一植株不同发育期叶片Bt蛋白的测定结果整体表现为:营养生长阶段跃生殖生长阶段跃成熟衰老阶段。研究结果为转Bt基因抗虫水稻适宜检测时期的选择提供了一定的参考。%In this study, the research materials were insect-resistant rice restorer line 9311(Bt) and its F1 Hybrids, and the negative control was non-GMO 9311. By using ELISA method, we studied the expression of soluble protein and Bt toxic protein in plants. The experimental results provided a scientific basis for the safety supervision of transgenic Bt rice. In general, with the transfer of the exogenous Bt gene, the content of soluble protein of rice did not change significantly. In different periods of growth stages and different tissues, the content of Bt toxic protein of insect-resistant restorer line 9311(Bt) was much higher than that of F1(Bt) hybrids. In different tissues of transgenic rice, the concentration of Bt protein expression was as follows:leaves跃endosperms跃glumes and stems跃roots. In different growth stages, Bt protein contents of leaves were as follows:the vegetative growth stage跃the reproductive growth stage跃the mature stage. The result of the research provided a certain reference for appropriate detection period of Bt-transgenic insect-resistant rice.

  5. 4个火焰兰属内及属间杂交新组合F1代核型分析%Karyotype Analysis of Four Intragenus and Intergenus Hybridized Combinations F 1 Generation of Renanthera

    Institute of Scientific and Technical Information of China (English)

    曹雯静; 李虹侠; 唐源江; 吴坤林

    2014-01-01

    The chromosome number and karyotype of four hybridized combinations F1 generation including three intragenus combinations of Renanthera and one intergenus combination of Renanthera with Vanda were studied with normal squashing method in this paper. The results showed that the chromosome number of R. coccinea ‘Qi Lin’, R. coccinea ‘Qi Lin’ ´ R. sinica, (R. imschootiana´R. sinica)´R. coccinea‘Qi Lin’ and R. imschootiana´Vanda coerulescens was 37, 57, 54, 38, and the karyotypes of these were formulated as 2n=2x+1=37=22m(2sat)+15sm(4sat), 2n=3x=57=39m(3sat)+12sm(3sat)+3st+3T, 2n=3x=54=9M+27m+12sm+3st+3T, 2n=2x=38=6M+14m+16sm+2T in turn. Asysmmetry coefficient of nuclear is 48.76, 60.50, 51.60, 65.87 respectively. The karyotypes of all examined belonged to 2B type of Stebbins. Aneuploid and polyploid were found in these hybridized combinations. The karyotypes of four F 1 generations were richer in variation.%采用染色体常规压片法对兰科Orchidaceae火焰兰属Renanthera内3个及与万代兰属Vanda间1个共4个杂交新组合F 1代核型进行研究。结果表明:4个杂交新组合F 1代中,麒麟火焰兰R. coccinea‘Qi Lin’、麒麟火焰兰×中华火焰兰R. sinica、(云南火焰兰R. imschootiana×中华火焰兰)×麒麟火焰兰、云南火焰兰×小花万代兰Vanda coerulescens的染色体数目依次为37、57、54、38;核型公式分别为:2n=2x+1=37=22m (2sat)+15sm (4sat)、2n=3x=57=39m (3sat)+12sm (3sat)+3st+3T、2n=3x=54=9M+27m+12sm+3st+3T、2n=2x=38=6M+14m+16sm+2T。核型不对称系数依次为:48.76、60.50、51.60、65.87。按Stebbins分类,均为“2B”型。4个新组合中,染色体存在非整倍体(三体)及多倍体(3×)现象,核形态显示了杂交新组合F 1代较丰富的变异特点。

  6. Larval salivary glue protein heterosis and dosage compensation among the interspecific F1 hybrids of Drosophila nasuta nasuta and Drosophila nasuta albomicans

    Directory of Open Access Journals (Sweden)

    Raghavan Prithi

    2016-01-01

    Full Text Available Reciprocal cross effects with respect to larval salivary secretory protein levels were studied in the interspecific fertile reciprocal hybrids by crossing Drosophila nasuta nasuta, and Drosophila nasuta albomicans. These proteins are produced copiously during the third larval instar stage and are believed to play a role in the attachment of pupa to the substratum prior to pupariation as well as in insect immunity. Quantitative variations were encountered among the reciprocal hybrids. Significant heterosis was observed between D. n. nasuta and the F1 hybrid female of a cross between D. n. albomicans female and D. n. nasuta male (21.39% while the F1 hybrids of a cross between D. n. nasuta female and D. n. albomicans male showed a marginal increase (4.24% from the mid parent level. The glue secretions were correlated to total cell number but independent of gland size. SDS PAGE revealed a considerable heterosis with respect to X-linked protein fractions. Here we report sex specific biochemical heterosis. However the X-linked fractions undergo dosage compensation in both parents and hybrids indicating strict regulatory control.

  7. Differential predation on tadpoles influences the potential effects of hybridization between Hyla cinerea and Hyla gratiosa

    Science.gov (United States)

    Gunzburger, M.S.

    2005-01-01

    Long-term effects of hybridization and introgression are influenced by performance of hybrids in habitats of parental species. The treefrogs Hyla cinerea and Hyla gratiosa, which typically breed in permanent and temporary habitats, respectively, have occasionally hybridized throughout the Southeastern United States. To predict in which of the parental habitats effects of hybridization might be strongest, I performed experiments to evaluate predation on tadpoles of H. cinerea, H. gratiosa, and F1 hybrids with predators typical of the breeding habitats of the parental species. Hybrid tadpoles had lower survival with sunfish than odonate naiad (dragonfly) predators and tended to increase hiding behavior in response to sunfish predation. Tadpoles of H. gratiosa also had higher survival with odonates than sunfish, but H. cinerea had similar survival with both predator types. These results suggest that hybrids are most likely to survive and return to breed in temporary habitats used by H. gratiosa. Thus, hybridization and introgression might be more likely to have adverse effects on populations of H. gratiosa than H. cinerea. Copyright 2005 Society for the Study of Amphibians and Reptiles.

  8. Partial resistance to Erwinia carotovora SUBSP. carotovora and plant vigour among F1 hybrids of Zantedeschia cultivars

    NARCIS (Netherlands)

    Snijder, R.C.; Tuyl, van J.M.

    2004-01-01

    The potential of breeding Zantedeschia cultivars for resistance to soft rot caused by Erwinia carotovora subsp. carotovora (syn. Pectobacterium carotovorum subsp. carotovorum) was determined. Hybrids of six cultivars (`Back Magic`, Galaxy`, Pink Persuasion`, Sensation`, `Treasure` and `Florex Gold`)

  9. COMPARATIVE RESEARCHE REGARDING METABOLIC PROFILE OF THE CALIFORNIAN, NEW ZEALAND WHITE, GRAND CHINCHILLA MEAT RABIT BREEDS AND THE F1 NZCH HYBRIDS

    Directory of Open Access Journals (Sweden)

    DANIELA-MARCELA TOBĂ (GOINA

    2013-12-01

    Full Text Available Precious biological characteristics of rabbits make their breeding a very profitable occupation. The rabbit meat, organoleptically same to the white meat, is rich in proteins, but low in fats. Biological researched done in direction to elucidate the biochemical systems that are the basis for organism physiological processes, have revealed that the level in which this process are develop directly influence the rabbits productivity capacity. 60 rabbit’s heads was used as biological material, distributed in: 15 Californian, 15 New Zeeland White, 15 Grand Chinchilla and 15 F1NZCH hybrids obtained from cross-breeding the New Zeeland White as maternal form and Grand Chinchilla as paternal form. Blood was sampled from the rabbit and was biochemical analyzed. The studied indices were: total protein, albumin, urea, uric acid, creatinine, total bilirubine, cholesterol, triglyceride and glucose. The experimental lot formed from F1 NZCH hybrids registered a concentration of 2.1 mg/dl uric acid, and in the other three lots the concentration was under 2 mg/dl. In all four lots, uric acid value was in normal limits. The determined creatinine registered very low values, under 1 mg/dl, at the low limit of reference values. At hybrids from New Zeeland White as maternal form and Grand Chinchilla as paternal form, in equal environmental conditions, the serum biochemical analysis haven’t registered significant differences compared to pure breeds individuals.

  10. Production of Hybrid F1 Between Avena magna and Avena nuda and It's Identification%四倍体大燕麦×六倍体裸燕麦的杂种F1的产生及鉴定

    Institute of Scientific and Technical Information of China (English)

    赵云云; 周小梅; 杨才

    2003-01-01

    本研究以四倍体大燕麦(Avena magna L.)做母本,六倍体裸燕麦(Avena nuda L.)做父本进行杂交,利用幼胚拯救技术获得了杂种F1,并对其后代形态特征进行了观察;对杂种F1同工酶图谱和DNA指纹图谱进行了分析.杂种F1形态特征偏亲本或介于双亲之间;同工酶研究表明多数F1具有双亲互补酶带;RAPD分析不同引物扩增产物F1呈共显性或偏父、偏母.这些结果表明F1为真杂种.

  11. Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers

    Institute of Scientific and Technical Information of China (English)

    YAO Yan; LU Yong-gen; LIU Xiang-dong; FENG Jiu-huan; ZHANG Gui-quan

    2006-01-01

    S-a, S-b and S-c are three loci for F1 pollen sterility in cultivated rice (Oryza sativa L.). Taichung 65 (T65) is all Sj/Sj at these three loci, while its F1 pollen sterile near-isogenic lines, TISL2 (S-b), TISL4 (S-a) and TISL5 (S-c) is Si/Si according to their respective sterility locus. Using SSR molecular marker to detect the segregation of the allele Si and Sj in pollen calli population induced from different hybrid F1, which have different pollen sterility locus, showed that the segregation of allele Si and Sj was distorted. The distorted direction of pollen calli population in vitro was not the same as F2 population in vivo. The quantities of pollen callus carrying Sj were much more than that of carrying Si at S-a and S-c locus, the ratio of Si and Sj were 1:4.81 and 1:1.96 respectively. But the opposite tendency was observed at S-b locus, the ratio of Si and Sj being 1:0.35. At the same time, all these results were undisturbed by either culture medium or culture period.

  12. Heterosis Increases Fertility, Fecundity, and Survival of Laboratory-Produced F1 Hybrid Males of the Malaria Mosquito Anopheles coluzzii

    Science.gov (United States)

    Ekechukwu, Nkiru E.; Baeshen, Rowida; Traorè, Sékou F.; Coulibaly, Mamadou; Diabate, Abdoulaye; Catteruccia, Flaminia; Tripet, Frédéric

    2015-01-01

    The success of vector control strategies aiming to decrease disease transmission via the release of sterile or genetically-modified male mosquitoes critically depends on mating between laboratory-reared males and wild females. Unfortunately, mosquito colonization, laboratory rearing, and genetic manipulations can all negatively affect male competitiveness. Heterosis is commonly used to produce domestic animals with enhanced vigor and homogenous genetic background and could therefore potentially improve the mating performance of mass-reared male mosquitoes. Here, we produced enhanced hybrid males of the malaria mosquito Anopheles coluzzii by crossing two strains colonized >35 and 8 years ago. We compared the amount of sperm and mating plug proteins they transferred to females, as well as their insemination rate, reproductive success and longevity under various experimental conditions. Across experiments, widespread adaptations to laboratory mating were detected in the older strain. In large-group mating experiments, no overall hybrid advantage in insemination rates and the amount of sperm and accessory gland proteins transferred to females was detected. Despite higher sperm activity, hybrid males did not appear more fecund. However, individual-male mating and laboratory-swarm experiments revealed that hybrid males, while inseminating fewer females than older inbred males, were significantly more fertile, producing larger mating plugs and drastically increasing female fecundity. Heterotic males also showed increased longevity. These results validate the use of heterosis for creating hybrid males with improved fitness from long-established inbred laboratory strains. Therefore, this simple approach could facilitate disease control strategies based on male mosquito releases with important ultimate benefits to human health. PMID:26497140

  13. Relationship Between F1, F2 Hybrid Yield, Heterosis and Genetic Distance Measured by Molecular Markers and Parent Performance in Cotton

    Institute of Scientific and Technical Information of China (English)

    WU Yao-ting; ZHANG Tian-zhen; ZHU Xie-fei; WANG Guang-ming

    2002-01-01

    Genetic distance among 36 cotton cultivars measured by molecular markers of RAPDs, ISSRs,and SSRs was from 0.0701 to 0.4255 with the mean of 0.2844, and from 2.18 to 12.60 with the mean of 7.04 based on the genotype performance in two-year field experiments, which has a significant positive correlation (r = 0.3350). The correlative coefficients for boll number per plant, boll weight, yield per plant, lint percent and lint yield per plant were 0. 8035,0. 8877,0. 7135,0. 9640 and 0. 8956 between F1 and F2 hybrid performance assessed by three-environment field experiments, respectively. The mean of F1 and F2 hybrid heterosis of yield per plant and lint yield per plant were 13.62%, 16.31%, 7.90% and 9.02%, and the correlative coefficients between them were 0.3689 and 0.3787, respectively. The correlation between the genetic distance and heterosis was low, and influenced directly by the selected parents.

  14. Performance and variability patterns in wood properties and growth traits in the parents, F1 and F2 generation hybrid clones of Populus deltoides

    Institute of Scientific and Technical Information of China (English)

    P. K. Pande; R. C. Dhiman

    2011-01-01

    The performance and variability patterns in the wood ele- ment's dimensions, specific gravity and growth parameters namely ramet height and GBH were evaluated in 16 clones of parents, F1 and F2 hy- brids of Populus deltoides Bartr. Ex Marsh. Ramet radial variations were non-significant, while inter-clonal variations due to interaction of clone/replication were significant for all the wood traits except vessel element length. Inter-clonal variations were significant only for fiber length and fiber wall thickness. Fiber length and specific gravity were significantly higher in female, while wall thickness and vessel element length were higher in male clones. Female parents (G48 and S7C8) showed higher flber length and specific gravity than of the male parent (G3), while vessel diameter and wall thickness were higher in male par- ent (G3). There is not much difference in fiber length and vessel ele- ment's dimensions among the parents, F1 and F2 generation hybrid clones. Specific gravity did not showed any trend for parents, F1 and F2 generations. Generally female clones showed higher growth rate. Broad sense heritability for wood traits ranged from 0.143 (fiber length) to 0.505 (fiber wall thickness), while for growth Waits it was 0.374 (GBH) and 0.418 (height). Genetic gain for all the wood and growth traits was positive for most of the wood waits. The highly divergent male clone (78) and female clones (S7C8, G48, W/A 49) in number of combinations could be used for developing new hybrids of desired wood traits to de- velop new clones.

  15. Genotypic Resistance of F1 Cotton Hybrids by Inoculation with Different Virulent Isolates of the Fungus Verticillium Dahliae Klebahn

    Institute of Scientific and Technical Information of China (English)

    AMANTURDIEV Alisher; KIM Robert

    2008-01-01

    @@ The plant pathogen Verticilliurn dahliae causes severe cotton losses in Uzbekistan.To create cotton varieties that are resistant to the more virulent races of V.dahliae we wanted to determine genotypic resistance of varieties and lines of cotton to more virulent isolates of different geographic populations of the fungus V.dahliae isolated from different cotton varieties in Uzbekistan and determine the inheritance of wilt resistance with hybrids of the first generation to various isolates of V.dahliae.

  16. The use of combining ability analysis to identify elite parents for Artemisia annua F1 hybrid production.

    Directory of Open Access Journals (Sweden)

    Theresa Townsend

    Full Text Available Artemisia annua is an important medicinal crop used for the production of the anti-malarial compound artemisinin. In order to assist in the production of affordable high quality artemisinin we have carried out an A. annua breeding programme aimed at improving artemisinin concentration and biomass. Here we report on a combining ability analysis of a diallel cross to identify robust parental lines for hybrid breeding. The parental lines were selected based on a range of phenotypic traits to encourage heterosis. The general combining ability (GCA values for the diallel parental lines correlated to the positive alleles of quantitative trait loci (QTL in the same parents indicating the presence of beneficial alleles that contribute to parental performance. Hybrids generated from crossing specific parental lines with good GCA were identified as having an increase in both artemisinin concentration and biomass when grown either in glasshouse or experimental field trials and compared to controls. This study demonstrates that combining ability as determined by a diallel cross can be used to identify elite parents for the production of improved A. annua hybrids. Furthermore, the selection of material for breeding using this approach was found to be consistent with our QTL-based molecular breeding approach.

  17. Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis.

    Science.gov (United States)

    Czernicka, Małgorzata; Pławiak, Jarosław; Muras, Piotr

    2014-01-01

    Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent--B. nana and 12 hybrids and cluster II grouped the male parent--B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes.

  18. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 2

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2013-07-01

    Full Text Available By using an incomplete factorial mating design between twenty Pinus strobus L. female and seven P. wallichiana Jack. male trees, a number of 34 full-sib families were obtained. The objective of this experiment was to combine the fast growing of the former species with moderately to high resistance to blister rust (Cronartium ribicola Fich. in Rabenh. of the latter one. The hybrid families were artificially inoculated at age two, and field planted at age four. The plantation took place within an old black current (Ribes nigrum L. heavy infected by blister rust. In order to provoke new infections, this time naturally, the pine rows were planted in between the black currant ones. Diameters at breast height, tree height, tree growth rate volume, stem straightness and tree survivals were the traits measured at age 32 from seed. The first trial thinning was simultaneously applied with the present measurements. The average tree survival was 74.8% in hybrids, 8.3% in Pinus strobus and 27.8% in P. wallichiana. Highly significant (p < 0.01 differences were found between hybrid families for all traits except stem straightness. Genetic coefficient of variation at family level was 13.7% for tree volume growth rate and 15.9% for tree survival, but only 2.1% for tree straightness. Broad-sense family heritability estimates were 0.530 for diameter at breast height, 0.596 for stem height, and 0.564 for stem volume growth rate, 0.166 for stem straightness, and 0.539 for tree survivals. Similarly, the individual tree narrow-sense heritability estimates were 0.138 for diameter at breast height, 0.209 for stem height, 0.149 for stem volume growth rate, and 0.022 for stem straightness. If the best 5, 10 and 15 of 34 families were selected, a genetic gain of 17.7%, 13.4% and 10.2%, respectively, may be achieved in tree survival or blister rust resistance. Similarly, if the best 5%, 10% and 15% individuals within the best hybrid families were selected, a genetic gain of

  19. Growth, straightness and survival at age 32 in a Pinus strobus x P. wallichiana F1 hybrid population (Experiment 2

    Directory of Open Access Journals (Sweden)

    Ioan Blada

    2013-05-01

    Full Text Available By using an incomplete factorial mating design between twentyPinus strobus L. female and seven P. wallichiana Jack. male trees, a number of 34 full-sib families were obtained. The objective of this experiment was to combine the fast growing of the former species with moderately to high resistance to blister rust (Cronartium ribicola Fich. in Rabenh. of the latter one. The hybrid families were artificially inoculated at age two, and field planted at age four. The plantation took place within an old black current (Ribes nigrum L. heavy infected by blister rust. In order to provoke new infections, this time naturally, the pine rows were planted in between theblack currant ones. Diameters at breast height, tree height, tree growth rate volume, stem straightness and tree survivals were the traits measured at age 32 from seed. The first trial thinning was simultaneously applied with the present measurements. The average tree survival was 74.8% in hybrids, 8.3% in Pinus strobus and 27.8% in P. wallichiana. Highly significant (p < 0.01 differences were found between hybrid families for all traits except stem straightness. Genetic coefficient of variation at family level was 13.7% fortree volume growth rate and 15.9% for tree survival, but only 2.1% for tree straightness. Broad-sense family heritability estimates were 0.530 for diameter at breast height, 0.596 for stem height, and 0.564 for stem volume growth rate, 0.166 for stem straightness, and 0.539 for tree survivals. Similarly, the individual tree narrow-sense heritability estimates were 0.138 for diameter at breast height, 0.209 for stem height, 0.149 for stem volume growth rate, and 0.022 for stem straightness. If the best 5, 10 and 15 of 34 families wereselected, a genetic gain of 17.7%, 13.4% and 10.2%, respectively, may be achieved in tree survival or blister rust resistance. Similarly, if the best 5%, 10% and 15% individuals within the best hybrid families were selected, a genetic gain of 4

  20. 紫贻贝和厚壳贻贝杂交及F1代杂交优势初探%A primary study on hybridization of Mytilus galloprovincialis, Mytilus coruscus, heterosis of F1 generation

    Institute of Scientific and Technical Information of China (English)

    常抗美; 刘慧慧; 李家乐; 沈玉帮

    2008-01-01

    2005-2007年对紫贻贝(Mytilus gallo provincialis)和厚壳贻贝(Mytilus coruscus)种间进行了杂交生产性试验,通过亲贝强化培育、确认雌雄亲贝、改革育苗水体交换方法、投喂混合单细胞藻类、流水培养附着稚贝等技术,获得F1代.结果表明,正交F1代幼虫的成活率明显高于反交F1代和厚壳贻贝,在幼虫培育前期与紫贻贝的成活率相当,但随着幼虫的发育有高于紫贻贝的趋势.正交F1代幼虫的壳长和壳高在培育阶段的前期生长与紫贻贝相当,低于厚壳贻贝,高于反交F1代;但后期的生长明显增快,并高于反交F1代和其他两种贻贝.各试验组贻贝海区养殖的成活率在90%以上,正交F1代最高,显著高于反交F1代.各试验组生长特性,正交F1代紫贻贝厚壳贻贝反交F1代.其中正交F1代获附着稚贝3.77×109 ind.正交F1除在孵化率上低于紫贻贝、厚壳贻贝外,在壳长、壳高、成活率等方面指标具有一定杂交优势,而反交F1代的杂交优势不明显.

  1. Results after 19 years of testing in a Pinus strobus x P. wallichiana F1 hybrid population

    Directory of Open Access Journals (Sweden)

    I. Blada

    2013-12-01

    Full Text Available Full-sib families were generated from a factorial design between seven female trees of P. strobus and four male trees of P. wallichiana to combine the rapid growth of former species with high resistance to blisterrust (Cronartium ribicola of the latter one. At age two the hybrid families were artificially inoculated with blister-rust and at age six theywere field planted. Total height growth, diameter at 1.3 m, basal area, stem volume, stem straightness and tree survival were measuredat age 19 after planting. Significant (p<0.05 and highly significant (p<0.01; p<0.001 differences among female effects were found for all tested traits including survival suggesting that nuclear additive genescontrolled these traits. Male effects were significant only for stem straightness. In all but one trait the σ2GCA: σ2SCA and σ2GCA-F : σ2GCA-M ratios were in favour of additive variance suggestingthat this variance should be used in the breeding strategy. Narrow-sense heritability at family level was 0.778 for volume growth rateand 0.861 for survival; such high values indicate that the breeding programme, has good chances of success. Two of the 11 tested parentsexhibited significant positive g.c.a. effects for survival after heavy blister-rust inoculation. One parent exhibited highly significantpositive g.c.a. effects for volume growth rate. The three good general combiners should be taken into account in the breeding programme.Estimate of the high-parent heterosis was negative for the volume but positive for survival. For volume, the hybrid mean was 5.9% lowerthan the mean of the eastern white pine but 85.4% greater than blue pine bulk lot for survival. Estimate of low-parent heterosis for survivalwas also positive; i. e. the hybrid population mean was 537.5% greater than eastern white pine. If the hybrids will be used in operationalplanting programs, a variable genetic gain between 6.0 and 10.5% for volume and between 6

  2. Results after 19 years of testing in a Pinus strobus x P. wallichiana F1 hybrid population

    Directory of Open Access Journals (Sweden)

    I. Blada

    2013-12-01

    Full Text Available Full-sib families were generated from a factorial design between seven female trees of P. strobus and four male trees of P. wallichiana to combine the rapid growth of former species with high resistance to blisterrust (Cronartium ribicola of the latter one. At age two the hybrid families were artificially inoculated with blister-rust and at age six theywere field planted. Total height growth, diameter at 1.3 m, basal area, stem volume, stem straightness and tree survival were measuredat age 19 after planting. Significant (p<0.05 and highly significant (p<0.01; p<0.001 differences among female effects were found for all tested traits including survival suggesting that nuclear additive genescontrolled these traits. Male effects were significant only for stem straightness. In all but one trait the σ2GCA: σ2SCA and σ2GCA-F : σ2GCA-M ratios were in favour of additive variance suggestingthat this variance should be used in the breeding strategy. Narrow-sense heritability at family level was 0.778 for volume growth rateand 0.861 for survival; such high values indicate that the breeding programme, has good chances of success. Two of the 11 tested parentsexhibited significant positive g.c.a. effects for survival after heavy blister-rust inoculation. One parent exhibited highly significantpositive g.c.a. effects for volume growth rate. The three good general combiners should be taken into account in the breeding programme.Estimate of the high-parent heterosis was negative for the volume but positive for survival. For volume, the hybrid mean was 5.9% lowerthan the mean of the eastern white pine but 85.4% greater than blue pine bulk lot for survival. Estimate of low-parent heterosis for survivalwas also positive; i. e. the hybrid population mean was 537.5% greater than eastern white pine. If the hybrids will be used in operationalplanting programs, a variable genetic gain between 6.0 and 10.5% for volume and between 6

  3. A triple mutation in the a subunit of the Escherichia coli/Propionigenium modestum F1Fo ATPase hybrid causes a switch from Na+ stimulation to Na+ inhibition.

    Science.gov (United States)

    Kaim, G; Dimroth, P

    1998-03-31

    Previously we have shown that the Na+-translocating Escherichia coli (F1-delta)/Propionigenium modestum (Fo+delta) hybrid ATPase acquires a Na+-independent phenotype by the c subunit double mutation F84L, L87V that is reflected by Na+-independent growth of the mutant strain MPC8487 on succinate [Kaim, G., and Dimroth, P. (1995) J. Mol. Biol. 253, 726-738]. Here we describe a new class of mutants that were obtained by random mutagenesis and screening for Na+-independent growth on succinate. All six mutants of the new class contained four mutations in the a subunit (S89P, K220R, V264E, I278N). Results from site-specific mutagenesis revealed that the substitutions K220R, V264E, and I278N were sufficient to create the new phenotype. The resulting E. coli mutant strain MPA762 could only grow in the absence but not in the presence of Na+ ions on succinate minimal medium. This effect of Na+ ions on growth correlated with a Na+-specific inhibition of the mutant ATPase. The Ki for NaCl was 1. 5 mM at pH 6.5, similar to the Km for NaCl in activating the parent hybrid ATPase at this pH. On the other hand, activation by Li+ ions was retained in the new mutant ATPase. In the absence of Na+ or Li+, the mutant enzyme had the same pH optimum at pH 6.5 and twice the specific activity as the parent hybrid ATPase. In accordance with the kinetic data, the reconstituted mutant ATPase catalyzed H+ or Li+ transport but no Na+ transport. These results show for the first time that the coupling ion selectivity of F1Fo ATPases is determined by structural elements not only of the c subunit but also of the a subunit.

  4. 制干辣椒F1代中色素含量的变异和遗传%Variation and Inheritance of Pigment Content in F1 Hybrids of Red Pepper for Grinding

    Institute of Scientific and Technical Information of China (English)

    Todorova V.; Todorov Y.; 徐乃林; 上官金虎; 庄灿然

    2006-01-01

    研究了2个制干红辣椒F1代中色素含量的变异和遗传.以Gorogled 6为试验母本,Negral和Kalocsai 801分别为试验父本进行杂交.颜色的变异性在2个F1代表现出加性遗传,但也有一种例外.Gorogled 6×Negral的F1代极显著地表明色素含量由加性值决定.第2个F1代即Gorogled 6×Kalocsai801仅在1997年表明了与上面第1个F1代相似的结果.在本试验中,F1代色素含量的遗传用d/a比值(1.75~7.96)来表示,并显示出这种性状的超显性遗传.假设和实际的杂种优势作用极显著.

  5. Genetic improvement on Chinese shrimp (Fenneropenaeuschinensis): growth and viability performance in F1 hybrids of different populations

    Institute of Scientific and Technical Information of China (English)

    TIAN Yi; KONG Jie; LI Wendong; LUAN Sheng; YANG Cuihua; WANG Qingyin

    2008-01-01

    Fenneropenaeus chinensis distributed in the Yellow Sea and Bohai Sea of China and the west coast of the Korean Peninsula. Different geographical populations represent potentially different genetic resources. To learn further the characteristics of different geographical population, crosses among two wild and three farmed populations were produced. The two wild populations were from the Yellow Sea and Bohai Sea (WYP), and the west coast of the Korean Peninsula and coast (WKN). The three farmed populations included the offspring of first generation of wild shrimp from coast in Korea (FKN), the Huang Hai (the Yellow Sea in Chinese) No. 1 (HH 1), and JK98. The phenotypes growth and survival rates of these populations were compared to confirm the feasibility for crossbreeding. The body length (BL), carapace length (CL), carapace width (CW), height of the second and third abdominal segment (HST), width of the second and third abdominal segment (WST), length of the first abdominal segment (LF), length of the last abdominal segment (LL), live body weight (BW), and survival rate were measured. Different combinations were statistically performed with ANOVA and Duncan's Multiple significant difference (P<0.05) in BL, CL, HST, LL, and BW; and insignificant difference (P0.05) in other growth traits and survival rate. The results of Duncan's Multiple Range Test are that BL and CL of was the best combination in all growth traits. Therefore, hybridization can introduce the variation to base populations. The systematic selection program based on additive genetic performance may be more effective than crossbreeding.

  6. 番茄新品种瑞丰的选育%A New Tomato F1 Hybrid - ‘Ruifeng'

    Institute of Scientific and Technical Information of China (English)

    王永珍; 张剑国

    2011-01-01

    Ruifeng is a new tomato Fi hybrid developed by crossing inbred line J3-106 as female parent and Y145-105 as male parent. It is of mid maturity. Its plant is indeterminate with high resistance to ToMV. Its ripe fruit is of oblate shape and red in color. It is tolerant to storage and transportation. The single fruit weight is 200-300 g. It has less deformed fruit. Its fruit has good commercial quality. Its yield is about 75 t ? Hm" . It is suitable to be cultivated in open fields all over Shanxi Province.%瑞丰是以自交系J3-106作母本,以Y145-105作父本配制而成的番茄一代杂种.无限生长类型,中熟,高抗番茄花叶病毒病( ToMV).果实高扁圆形,成熟果大红色,耐贮运,单果质量200~300g,不易产生畸裂果,商品性好,一般每667 m2产量5 000 kg左右,适于山西地区露地栽培.

  7. F1 (CBA×C57) mice show superior hearing in old age relative to their parental strains: hybrid vigor or a new animal model for "golden ears"?

    Science.gov (United States)

    Frisina, Robert D; Singh, Ameet; Bak, Matthew; Bozorg, Sara; Seth, Rahul; Zhu, Xiaoxia

    2011-09-01

    Age-related hearing loss - presbycusis - is the most common communication problem and third most prevalent chronic medical disorder of the aged. The CBA and C57BL/6 mouse strains are useful for studying features of presbycusis. The CBA loses its hearing slowly, like most humans. Because the C57 develops a rapid, high frequency hearing loss by middle age, it has an "old" ear but a relatively young brain, a model that helps separate peripheral (cochlear) from central (brain) etiologies. This field of sensory neuroscience lacks a good mouse model for the 5-10% of aged humans with normal cochlear sensitivity, but who have trouble perceiving speech in background noise. We hypothesized that F1 (CBA×C57) hybrids would have better hearing than either parental strain. Measurements of peripheral auditory sensitivity supported this hypothesis, however, a rapid decline in the auditory efferent feedback system, did not. Therefore, F1s might be an optimal model for studying cases where the peripheral hearing is quite good in old age; thereby allowing isolation of central auditory changes due to brain neurodegeneration.

  8. 番茄新品种浙杂806的选育%A New Tomato F1 Hybrid — ‘Zheza 806’

    Institute of Scientific and Technical Information of China (English)

    杨悦俭; 马忠翼; 周国智

    2001-01-01

    Zheza 806 is a new indeterminate F1 hybrid. Its female parent line is T217 that selected from the progenies of the cross between processing variety Zhehong 20 and Manapal Tm-2nv introduced from US. T217 has the gene to resist TMV and double recessive characteristics — yellowish leaf and green stem for easy to distinguish false F1 seedlings. Male parent,87-122-4,is selected from Xiaojixin,a Shanghai local determinate variety. This hybrid F1 suits plastic house cultivation in winter and early spring season,as well as open field in spring and highland in summer. It has very good quality fruit with red color and high-resistance to TMV and mid-resistance to CMV. Its average yield is about 75 t*hm-2. The total plant acreage is around 1 000 hm2 in Zhejiang and some area nearby.%浙杂806的母本为品质极佳的加工品种浙红20和美国引进材料Manapal Tm-2nv的杂交后代经多代株系选育而成的T217,具有高抗TMV的Tm-2nv基因和黄绿叶、绿茎双隐性标记性状,可方便剔除假杂种。父本87-122-4是从上海地方品种小鸡心中系选而成。该杂种一代为无限生长类型,适合长江流域冬春大棚栽培,尤适春季露地和夏秋高山栽培,高抗TMV、中抗CMV,单果质量220 g,果实大红色、高圆形,可溶性固形物4.6 %,品质风味佳,每667 m2产量在5 000 kg左右,高产可达7 000 kg以上。在浙江、福建等地已推广1000 hm2。

  9. A screen for F1 hybrid male rescue reveals no major-effect hybrid lethality loci in the Drosophila melanogaster autosomal genome.

    Science.gov (United States)

    Cuykendall, Tawny N; Satyaki, P; Ji, Shuqing; Clay, Derek M; Edelman, Nathaniel B; Kimchy, Alexandra; Li, Ling-Hei; Nuzzo, Erin A; Parekh, Neil; Park, Suna; Barbash, Daniel A

    2014-10-27

    Hybrid sons between Drosophila melanogaster females and D. simulans males die as 3rd instar larvae. Two genes, D. melanogaster Hybrid male rescue (Hmr) on the X chromosome, and D. simulans Lethal hybrid rescue (Lhr) on chromosome II, interact to cause this lethality. Loss-of-function mutations in either gene suppress lethality, but several pieces of evidence suggest that additional factors are required for hybrid lethality. Here we screen the D. melanogaster autosomal genome by using the Bloomington Stock Center Deficiency kit to search for additional regions that can rescue hybrid male lethality. Our screen is designed to identify putative hybrid incompatibility (HI) genes similar to Hmr and Lhr which, when removed, are dominant suppressors of lethality. After screening 89% of the autosomal genome, we found no regions that rescue males to the adult stage. We did, however, identify several regions that rescue up to 13% of males to the pharate adult stage. This weak rescue suggests the presence of multiple minor-effect HI loci, but we were unable to map these loci to high resolution, presumably because weak rescue can be masked by genetic background effects. We attempted to test one candidate, the dosage compensation gene male specific lethal-3 (msl-3), by using RNA interference with short hairpin microRNA constructs targeted specifically against D. simulans msl-3 but failed to achieve knockdown, in part due to off-target effects. We conclude that the D. melanogaster autosomal genome likely does not contain additional major-effect HI loci. We also show that Hmr is insufficient to fully account for the lethality associated with the D. melanogaster X chromosome, suggesting that additional X-linked genes contribute to hybrid lethality. Copyright © 2014 Cuykendall et al.

  10. 不同氮效率茄子基因型及其杂种F1的氮素吸收特性%Characteristics of nitrogen uptake in eggplant genotypes with different nitrogen efficiency and their hybrid F1s

    Institute of Scientific and Technical Information of China (English)

    田松; 银婷; 陈雪平; 王彦华; 罗双霞; 申书兴

    2011-01-01

    In order to elucidate the heterosis on nitrogen uptake in the F1 hybrids, the nitrogen (N) absorption characteristics of the eggplant genotypes with different N efficiencies and their hybrid F1 s were studied. Under normal and low N stress conditions, root volume, root dry weight, N uptake, root activity, nitrate reductase activity and glutamine synthetase activity of leaves were studied in three typical eggplant genotypes with different N efficiencies and their hybrids. Compared with low N efficiency genotypes(L) ,the root volume, root dry weight, root activity, total N uptake per plant, nitrate reductase activity and glutamine synthetase activity of the cultivars with high N efficiency (H1 and H2) are all higher. In the three F1 hybrids, F1 -1 (L × H1 ), F1 -2 (L × H2), and F1 -3 (H1 × H2 ), the root volume, root dry weight , root activity, nitrate reductase activityof leaves, glutamine synthetase activity of leaves and total N uptake per plant of Hm and Hp are mostly positive, in which the hybrid F1 -3 has the best heterosis. Using the heterosis on N uptake in the F1 hybrids, it will be useful to improve the N metabolism and increase the N efficiency of the plant.%为阐明杂种一代在氮素吸收方面的优势,研究了不同氮效率茄子基因型及其杂种目的氮素吸收特性.试验以3个典型氮效率的茄子基因型及其F1代为材料,研究其在正常供氮和低氮胁迫条件下的根系体积、根系干重、氮素吸收总量、根系活力、硝酸还原酶活性及谷氨酰胺合成酶活性.结果表明.与高氮低效一低氮低效基因型L相比,氮高效基因型H1、H2的单株根系体积、根系干重、根系活力以及氮素吸收总量均较大;且具有较高的硝酸还原酶与谷氨酰胺合成酶活性.三个杂交组合F1-1(L×H1)、F1-2(L×H2)和F1-3(H1×H2)的单株根系体积、根系干重、根系活力、硝酸还原酶活性、谷氨酰胺合成酶活性以及氮素吸收总量的中亲优势(Hm)和

  11. Sunfish amphiphiles : Conceptually new carriers for DNA delivery

    NARCIS (Netherlands)

    Hulst, R; Muizebelt, [No Value; Oosting, P; van der Pol, C; Wagenaar, A; Smisterova, J; Bulten, E; Driessen, C; Hoekstra, D; Engberts, JBFN; Muizebelt, Inouk; Šmisterová, Jarmila

    2004-01-01

    A conceptually new class of cationic amphiphiles, Sunfish amphiphiles, designed for the delivery of genes into cells is introduced. Sunfish amphiphiles have two hydrophobic tails, connected at the 4- and the N-position to the cationic pyridinium headgroup. Two extreme morphologies visualised by back

  12. Male fitness of oilseed rape (¤Brassica napus¤), weedy ¤B-rapa¤ and their F1 hybrids when pollinating ¤B-rapa¤ seeds

    DEFF Research Database (Denmark)

    Pertl, M.; Hauser, T.P.; Damgaard, C.

    2002-01-01

    The likelihood that two species hybridise and backcross may depend strongly on environmental conditions, and possibly on competitive interactions between parents and hybrids. We studied the paternity of seeds produced by weedy Brassica rapa growing in mixtures with oilseed rape (B. napus...... is strongly influenced by their local frequencies, and that male fitness of F(1)hybrids, when pollinating B. rapa seeds, is low even when their female fitness (seed set) is high.......), respectively. F(1) and backcross offspring were produced mainly by a few B. rapa mother plants. The observed hybridisation and backcrossing frequencies were much lower than expected from our compilation of fitness components. Our results show that the male fitness of B. rapa, B. napus, and F(1) hybrids...

  13. Analysis of the meiosis in the F-1 hybrids of Longiflorum x Asiatic (LA) of lilies (Lilium) using genomic in situ hybridization

    NARCIS (Netherlands)

    Zhou, S.J.; Ramanna, M.S.; Visser, R.G.F.; Tuyl, van J.M.

    2008-01-01

    Longiflorum and Asiatic lilies of the genus Lilium of the family Liliaceae are two important groups of modem lily cultivars. One of the main trends of lily breeding is to realize introgression between these groups. With cut style pollination and embryo rescue, distant hybrids between the two groups

  14. Reciprocal exchange of minor components of type 1 and F1C fimbriae results in hybrid organelles with changed receptor specificities

    DEFF Research Database (Denmark)

    Klemm, P; Christiansen, Gunna; Kreft, B;

    1994-01-01

    , the sequence identity between the minor components of the type 1 and F1C fimbriae is only 34 to 41%. Type 1 fimbriae mediate agglutination of guinea pig erythrocytes, whereas F1C fimbriae do not confer agglutination of any types of erythrocytes tested. However, F1C fimbriae mediate specific adhesion...

  15. Cell physiology of mortality and immortality in a Nicotiana interspecific F1 hybrid complies with the quantitative balance between reactive oxygen and nitric oxide.

    Science.gov (United States)

    Yamamoto, Takumi; Shomura, Sachiko; Mino, Masanobu

    2017-03-01

    The cultured cell line, GTH4, of an interspecific F1 hybrid between Nicotiana gossei Domin and N. tabacum L. died after a shift in temperature from 37°C to 26°C. Fluctuations in the cellular amounts of reactive oxygen species (ROS) and nitric oxide (NO) were detected in GTH4 after the temperature shift, but not in the mutant, GTH4S, which did not die at 26°C presumably due to the lack of genetic factors involved in cell death. The removal of ROS or NO suppressed cell death in GTH4, suggesting that ROS and NO both acted as mediators of cell death. However, excess amounts of the superoxide anion (O2(-)) or NO alleviated cell death. A series of experiments using generators and scavengers of ROS and NO showed that O2(-) affected the cellular levels of NO, and vice versa, indicating that a quantitative balance between O2(-) and NO was important for hybrid cell death. The combination of NO and hydrogen peroxide (H2O2) was necessary and sufficient to initiate cell death in GTH4 and GTH4S. Hypoxia, which suppressed cell death in GTH4 at 26°C, reduced the generation of H2O2 and NO, but allowed for the production of O2(-), which acted as a suppressor and/or modulator of cell death. The activation of MAPK was involved in the generation of H2O2 in GTG4 cells under normoxic conditions, but promoted O2(-) generation under hypoxic conditions. More protective cellular conditions against ROS, as estimated by the expression levels of genes for ROS-scavenging enzymes, may be involved in the mechanisms responsible for the low cell death rate of GTH4 under hypoxic conditions. Copyright © 2017 Elsevier GmbH. All rights reserved.

  16. [Effect of oregano essential oil on the engraftment and development of Lewis carcinoma in F1 DBA C57 black hybrid mice].

    Science.gov (United States)

    Misharina, T A; Burlakova, E B; Fatkullina, L D; Alinkina, E S; Vorob'eva, A K; Medvedeva, I B; Erokhin, V N; Semenov, V A; Nagler, L G; Kozachenko, A I

    2013-01-01

    The effect of a low uptake dose of oregano essential oil with drinking water for three months (Origanum vulgare L.) on the degree of Lewis carcinoma engraftment and some parameters of oxidative stress has been studied in vivo using F1 DBA C57 Black hybrid mice. Oregano essential oil has been established to possess an anticancer activity. The degree of tumor engraftment decreased by 1.8 times, its size decreased by 1.5 times, and the development of tumor was significantly suppressed in sick mice under the effect of oregano essential oil. It was found that the uptake of essential oil did not affect the intensity of lipid peroxidation in the brain of mice and resulted in a significantly (by 36%) decreased content of secondary lipid oxidation products in the liver as shown in a reaction with thiobarbituric acid as compared to control subjects. The activity of antioxidant enzymes was found to increase after three months of essential oil uptake (by 1.5-3 times) as compared to the control group. This effect of essential oil supports the presence of bioantioxidant properties in this essential oil.

  17. Cultivation Trial of F1 Hybrids of Several Cabbage Sprouts%几个白菜薹杂种一代试种小结

    Institute of Scientific and Technical Information of China (English)

    陈利丹; 王先琳; 林馥芬

    2012-01-01

      为了充分了解6个新选育的白菜薹品种在华南地区的特征特性以及适应性,以雪莹为对照进行品比试验,试验中充分调查了各杂种一代的特征特性,连续采收统计产量,进行多重比较。试验结果表明,组合1002和对照雪莹产量高,适应性强,品质佳,适宜在广东地区推广。%  In order to fully understand the characteristics and adaptability of the six new cabbage sprouts cultivars in south China, we studied the characteristics of their F1 hybrids, and carried out the multiple comparison of their total yields, by taking the cultivar Xueying as control. The results showed that the combination 1002 and the control cultivar Xueying possessed high yield, strong adaptability and good quality, which could be promoted in Guangdong area.

  18. Relative role of flower color and scent on pollinator attraction: experimental tests using F1 and F2 hybrids of daylily and nightlily.

    Directory of Open Access Journals (Sweden)

    Shun K Hirota

    Full Text Available The daylily (Hemerocallis fulva and nightlily (H. citrina are typical examples of a butterfly-pollination system and a hawkmoth-pollination system, respectively. H. fulva has diurnal, reddish or orange-colored flowers and is mainly pollinated by diurnal swallowtail butterflies. H. citrina has nocturnal, yellowish flowers with a sweet fragrance and is pollinated by nocturnal hawkmoths. We evaluated the relative roles of flower color and scent on the evolutionary shift from a diurnally flowering ancestor to H. citrina. We conducted a series of experiments that mimic situations in which mutants differing in either flower color, floral scent or both appeared in a diurnally flowering population. An experimental array of 6 × 6 potted plants, mixed with 24 plants of H. fulva and 12 plants of either F1 or F2 hybrids, were placed in the field, and visitations of swallowtail butterflies and nocturnal hawkmoths were recorded with camcorders. Swallowtail butterflies preferentially visited reddish or orange-colored flowers and hawkmoths preferentially visited yellowish flowers. Neither swallowtail butterflies nor nocturnal hawkmoths showed significant preferences for overall scent emission. Our results suggest that mutations in flower color would be more relevant to the adaptive shift from a diurnally flowering ancestor to H. citrina than that in floral scent.

  19. 抗 TY 番茄新品种海纳178的选育%Breeding of Haina 178, a New Tomato F1 Hybrid Resistant to TY

    Institute of Scientific and Technical Information of China (English)

    顾海华; 吴玉华; 钮建平; 黄亮; 代国丽

    2016-01-01

    海纳178是以 K-1-4-5-1-3为母本,H-5-1-4-2-2为父本组配而成的番茄一代杂种。该品种为无限生长类型,植株生长健壮;成熟果大红色,色泽鲜亮;果硬、耐贮运,单果质量180 g 左右,中熟,抗黄化曲叶病毒病,植株综合抗逆性强。%Haina 178 is a new tomato F1 hybrid developed by crossing inbred line K-1-4-5-1-3 as female parent and H-5-1-4-2-2 as male parent. It is an unlimited growth type and grows vigorously. The matured fruit is big and red with brightcolor, and the fruit is hard, tolerant to storage and transportation. The average single fruit weight is about 180 g. And it is a middle -maturing cultivar. Besides, Haina 178 is also resistant to tomato yellow leaf curl virus disease. Therefore, it has strong comprehensive stress resistance.

  20. Monoterpene emissions from needles of hybrid larch F1 (Larix gmelinii var. japonica × Larix kaempferi) grown under elevated carbon dioxide and ozone

    Science.gov (United States)

    Mochizuki, Tomoki; Watanabe, Makoto; Koike, Takayoshi; Tani, Akira

    2017-01-01

    We measured monoterpene emissions from needles of hybrid larch F1 (Larix gmelinii var. japonica × Larix kaempferi) to evaluate the response of monoterpene emission rates and their composition to elevated CO2 (600 μmol mol-1) and O3 (60 nmol mol-1) conditions. The dominant monoterpenes were α-pinene and β-pinene. The emission rate of total monoterpenes significantly decreased under elevated CO2 conditions (P photosynthetically fixed carbon also significantly decreased under elevated CO2 conditions. By contrast, elevated O3 did not significantly affect the emission rate of total monoterpenes. The ratios of α-pinene/β-pinene, limonene/β-pinene, and myrcene/β-pinene were all significantly decreased by O3 exposure (P < 0.05). High reactivity of α-pinene, limonene, and myrcene when combining with O3 may be able to mitigate oxidative damage inside the larch needles. No significant combined effects of elevated CO2 and O3 on individual or total monoterpene emissions were detected.

  1. 早熟大白菜新品种夏白45的选育%New Chinese Cabbage F1 Hybrid - 'Xiabai 45'

    Institute of Scientific and Technical Information of China (English)

    宋聚红

    2001-01-01

    夏白45大白菜的母本是从晋菜3号中筛选出的直筒舒心型自交不亲和系87- 3-1,父本是从北早4号中选出的自交不亲和系90-7-5。该一代杂种极早熟,从播种到收获45~50d(天),抗病毒病和黑腐病,一般667m2产量为3000~4000kg,比对照夏阳50增产30%左右,适于在河北、山东、山西、河南等地推广。%Xiabai 45 is a new Chinese Cabbage F1 hybrid by cro ssing self-incompatible line 87-3-1 and 90-7-5. It has early maturity,dise ase resistance and high yield. The yield is about 45~60 t*hm-2,3 0 % higher than Xiayang 50. It is suitable to be grown in Hebei,Shandong ,Shanxi and Henan.

  2. 甘蓝新品种夏华2号的选育%A New Cabbage F1 Hybrid — ‘Xiahua No.2'

    Institute of Scientific and Technical Information of China (English)

    陈文辉; 方淑桂; 邵贵荣

    2011-01-01

    夏华2号是由细胞质雄性不育系NBB10-87和优良自交系107-1配制而成的甘蓝一代杂种.中熟,较耐热,从定植到采收70 d(天).株高32 cm,开展度60 cm,叶色灰绿,蜡粉中等,叶球扁圆,球高13.5 cm、球宽17 cm,中心柱长6 cm,单球质量1.1 kg,净菜率75%,每667 m2产量2 500~3 000 kg.适合福建省夏、秋季栽培.%'Xiahua No.2' is a new cabbage F1 hybrid developed by crossing cytoplasmic male sterile line NBB10-87 and inbred line 107-1. It is a mid-maturing and heat-resisting cultivar. It takes about 70 days from transplanting to harvest. The plant is about 32 cm in height, 60 cm in expansion diameter. It is green in color with medium wax powder. The head is flat and 13.5 cm in height, 17 cm in diameter. The average head weight is 1.1 kg and mean yield is 37.5-45.01 · hm-2. It is suitable for cultivation in summer and autumn in Fujian Province.

  3. Comportamento de somaclones de arroz derivados de híbridos da geração F1 para resistência à brusone Performance of rice somaclones derived from F1 hybrids to blast resistance

    Directory of Open Access Journals (Sweden)

    Leila Garcês de Araújo

    2002-05-01

    Full Text Available A indução de variabilidade genética em relação à resistência à brusone, utilizando cultura de tecidos como material, constitui uma das alternativas para a obtenção de novas fontes de genes de resistência. O objetivo deste estudo foi aumentar a freqüência de variantes usando como explante panículas imaturas da geração F1 de cruzamentos envolvendo fontes altamente suscetíveis e moderadamente resistentes à brusone como parentais. Somaclones de arroz derivados de plantas F1 dos cruzamentos Bluebelle/Araguaia e Maratelli/Basmati-370 foram avaliados, nas gerações avançadas, quanto à resistência à brusone e a algumas características agronômicas. Nos testes de inoculações em casa de vegetação, todos os somaclones, de ambos os cruzamentos, na geração R4, apresentaram alto grau de resistência aos patótipos IB-1 e IB-9. Alguns dos somaclones mantiveram-se resistentes na geração R5, em avaliações realizadas com alta pressão de brusone. No campo, os somaclones R5 e R6 mostraram alta freqüência de variação quanto à resistência à doença, altura da planta, produtividade, peso e tipo de grãos. Dois somaclones derivados do cruzamento Bluebelle/Araguaia e 31 somaclones derivados do cruzamento Maratelli/Basmati-370 foram identificados como novas fontes de resistência à brusone, e podem ser utilizados no programa de melhoramento de arroz irrigado.The induction of genetic variability, in relation to blast resistance, using tissue culture as a tool, constitutes one of the alternatives for obtaining novel resistance gene sources. The objective of this study was to increase the frequency of variants using immature panicles as explant from the F1 plants of crosses involving susceptible and moderately blast resistant sources as parents. Somaclones of rice derived from F1 plants of the crosses Bluebelle/Araguaia and Maratelli/Basmati-370 were assessed in advanced generations for blast resistance and some agronomic

  4. 番茄新品种金冠14的选育%A New Tomato F1 Hybrid-‘Jinguan 14’

    Institute of Scientific and Technical Information of China (English)

    杨国栋; 吕书文; 张馨宇

    2015-01-01

    金冠14是以自交系11-437为母本、11-504为父本育成的番茄一代杂种。植株为无限生长类型,第6~7节着生第1花序;成熟果粉红色,无绿果肩,果实扁圆形,硬度高,耐贮运,商品性好,平均单果质量202 g,可溶性固形物含量4.2%;耐低温弱光,平均产量6000 kg·(667 m2)-1,适于辽宁地区保护地种植。%‘Jinguan 14’is a new tomato F1 hybrid developed by crossing inbred line 11-437 as female parent and 11-504 as male parent. It belongs to infinite growth type,and the first inflorescence node sets on section 6-7th. The ripe fruits are pink in color without green neak. Its fruit is of oblate shape with high hardness. It is tolerant to storage and transportation,and has good commercial quality. The average single fruit weight is 202 g. The soluble solid content is 4.2%. It tolerant to low temperature and weak light. Its average yield is about 90 t·hm-2. It is suitable for protected cultivation in Liaoning Province.

  5. A New Cherry Tomato F1 Hybrid-‘Jinmei’%樱桃番茄新品种金美的选育

    Institute of Scientific and Technical Information of China (English)

    黄浅; 钱健康; 田炜; 刘杰; 李国臣

    2016-01-01

    ‘Jinmei’ is a new cherry tomato F1 hybrid developed by crossing ‘MY0216’as female parent and‘MY0310’as male parent. This variety belongs to indeterminate growth type. The fruit is of high round shape. The young fruit has no green shoulder,and the matured fruit is yellow in color. The average single fruit weight is about 17.6 g. Its fruit hardness is high. And it is tolerant to storage and transportation. The fruit tastes sweet & sour with good quality. It can yield about 45 t·hm-2. It is suitable to be planted in the greenhouse or open fields in Beijing,Liaoning,Henan,Shaanxi,Hunan,Jiangsu,Zhejiang and Hainan Provinces and etc. areas in spring.%金美是以MY0216为母本,以MY0310为父本育成的樱桃番茄一代杂种。无限生长类型,果实高圆形,幼果无绿果肩,成熟果黄色,平均单果质量17.6 g左右,果实硬度高,耐贮运,口味甜酸,品质优。每667 m2产量达3000 kg左右。可在北京、辽宁、河南、陕西、湖南、江苏、浙江和海南等适宜地区春季保护地或露地种植。

  6. A New Tomato F1 Hybrid -‘Longfan No.11’%番茄新品种陇番11号的选育

    Institute of Scientific and Technical Information of China (English)

    胡志峰; 邵景成; 张少丽

    2014-01-01

    ‘Longfan No.11’is a new F1 hybrid of middle-late maturing tomato developed by crossing 2032 as female parent and 2125 as male parent. It is a variety with indeterminate growth. Its first inflorescence setting at the 7-8th leaf nods. The matured fruit is round in shape and pink in peel color. The average single fruit weight is 195.2 g. The average fruit firmness is 1.02 kg·cm-2. It is tolerant to storage and transportation. It is resistant to leaf mold,and middle resistant to early blight. Its average yield is about 114 t·hm-2 in protected fields. It is suitable for cultivation in protected fields in northern China.%陇番11号是以2032为母本,以2125为父本配制而成的中晚熟番茄一代杂种。从开花到果实成熟需56 d(天)左右,无限生长类型,第7~8节出现第1花序,果实圆形,成熟果粉红色,平均单果质量195.2 g,果实硬度1.02 kg·cm-2,耐贮运;抗叶霉病,中抗早疫病;保护地栽培每株留4穗果时,每667 m2产量7600 kg 左右,适宜我国北方地区日光温室、塑料大棚等保护地栽培。

  7. A New Hot Pepper F1 Hybrid-‘Tianjiao No.13’%干椒新品种天椒13号的选育

    Institute of Scientific and Technical Information of China (English)

    霍建泰; 逯建平; 唐瑞永; 赵国珍; 高大伟

    2015-01-01

    ‘Tianjiao No.13’ is a new hot pepper F1 hybrid developed by crossing 10-37 as female parent and 10-178 as male parent. It is of medium maturity. The plant has medium growth vigor. Its fruit is of sheep-horn shape. The fruit is 17.5 cm in length and 3.2 cm in width. The single fresh fruit weight is about 35.4 g. The immature fruit is green,and ripen one is dark red in color. The clarity of the pigment is 15.8. It is suitable for the extraction of Capsanthin. It can yield about 6 t·hm-2 dry pepper,which is about 50% higher than that of‘Meiguohong’. Its resistance to virus disease,phytophthora blight,anthracnose and powdery mildew are stronger than that of the contrast ‘Meiguohong’. It is suitable to be planted in open fields in Gansu Province.%天椒13号是以10-37为母本,以10-178为父本配制而成的中熟高色价辣椒一代杂种。植株长势中等,果实羊角形,纵径17.5 cm,横径3.2 cm,鲜红椒平均单果质量35.4 g。青熟果绿色,老熟果深红色,色价15.8,适合提取辣椒红色素。每667 m2干椒产量400 kg左右,田间对病毒病、疫病、炭疽病及白粉病的抗性强于对照美国红,适宜甘肃省露地种植。

  8. 辣椒新品种宝龙2号的选育%A New Pepper F1 Hybrid-‘Baolong No.2’

    Institute of Scientific and Technical Information of China (English)

    李显日; 王广华; 刘玲; 金松子

    2015-01-01

    ‘Baolong No.2’is a new pepper F1 hybrid bred by crossing a cytoplasmic male sterile(CMS)line 741A as female parent with a restorer line 770C as male parent. This variety is of mid-early maturity. The first flower sets on the 8-9 th node. Its fruit is 22-26 cm in length,4.0-4.5 cm in diameter,and about 0.4 cm in flesh thickness. The single fruit weight is 85 g. Its fruit is of sheep-horn shape. It tastes a little pungency. The immature fruit is light green in color. It turns red when ripening. It has good commocial character and superior quality. Its yield is 61.5-66.0 t·hm-2. It is resistant to PMMoV and Phytophthora blight. It is suitable for protected cultivation in early spring and late autumn in northern China.%宝龙2号是以辣椒胞质雄性不育系741A为母本,恢复系770C为父本配制的辣椒一代杂种。该品种中早熟,始花节位为第8~9节,果长22~26 cm,果宽4.0~4.5 cm,果肉厚0.4 cm左右,单果质量85 g,果实羊角形,微辣,青熟果浅绿色,老熟果红色,商品性好,品质优良,每667 m2产量4100~4400 kg。抗PMMoV和疫病,适宜北方地区早春及秋延迟保护地栽培。

  9. 三系辣椒新品种报喜的选育%A New Hot Pepper F1 Hybrid “Herald Delight”

    Institute of Scientific and Technical Information of China (English)

    孟宝; 周阜宣; 戴祖云

    2012-01-01

    Herald Delight is a new hot pepper F1 hybrid bred by crossing of spicy bell pepper line as female parent and thick ball horn type pepper line as male parent. It bred by Anhui Jianghuai Horticultural Technology Co., LTD. The female parent jhsp-07-03 is from a male sterile line. The male parent jhhp-05-21 is selected from a mutation of the female parent of variety 301. Herald Delight is suitable for protected production. The average fruit weight is 53.7 g. The 667 m2 yield is 2 945 kg. It is very early variety produces wrinkled fruit with medium pungency.%报喜是安徽江淮园艺科技有限公司采用带有辣味的甜椒类型品种作母本,以大果泡椒作父本培育的杂交一代辣椒新品种.母本是辣甜椒经多代转育后形成的不育系,代号jhsp-07-03.父本为301辣椒品种中母本突变单株经多代选育后的稳定株系,代号jhhp-05-21.经多代试验表明,报喜适合大棚保护地栽培,平均单果质量53.7 g,平均667 m2产量为2 945 kg,极早熟,皮薄,果面较皱,辣味中等.

  10. A New Cherry Tomato F1 Hybrid ─‘Xiaokeai’%樱桃番茄新品种小可爱的选育

    Institute of Scientific and Technical Information of China (English)

    张战泓; 白占兵

    2013-01-01

    ‘Xiaokeai’ is a new cherry tomato F1hybrid developed by crossing inbred lines A58-5-12-3 as female parent and B49-5-3-1 as male parent.This variety belongs to unlimited growth type. The plant has dark green leaf and red fruit.The fruit is of round shape.The single fruit weight is 18-20 g with good taste.Its resistances to bacterial wilt,late blight and leaf mold are higher than that of the contrast‘Xiaolongnyu’.The average yield is about 45 t·hm-2in open field,and about 90 t·hm-2 in protected field. It is suitable for cultivation in southern China,where bacterial wilt occurred frequently.%小可爱是以A58-5-12-3为母本、B49-5-3-1为父本配制而成的樱桃番茄一代杂种。该品种属无限生长类型,叶色深绿,红果,果实圆形,单果质量18~20 g,酸甜适中,口感好。对番茄青枯病、晚疫病和叶霉病的抗性强于对照小龙女。早春露地栽培每667 m2平均产量3000 kg 左右,保护地栽培每667 m2平均产量6000 kg左右。高抗青枯病,适合南方青枯病高发地区栽培。

  11. Frozen F1's amidst a masterpiece of nature: new insights into the rare hybrid origin of gynogenesis in the Amazon molly (Poecilia formosa).

    Science.gov (United States)

    Rogers, S M; Vamosi, S M

    2010-12-01

    All-female 'species' of fish have been shown to be great models in ecological and evolutionary studies because of the insights they can provide into the origin and evolution of asexuality, the ecology of hybrids, associations between genotype and environment, and the maintenance of sex. Gynogenetic organisms that evolved from sexual ancestors, and combine the disadvantageous traits from sexuality and asexuality, have long baffled evolutionary biologists trying to understand their origin and persistence with their sympatric sexual counterparts. In this issue, a new study using an integrated molecular phylogenetic and classical genetic approach has uncovered compelling evidence regarding the obscure asexual origin of the Amazon molly, Poecilia formosa. By performing an extensive phylogeographic analysis, Stöck et al. (2010) provide evidence that the Amazon molly arose only once within its history, with monophyly being strongly supported by mitochondrial DNA and microsatellite analyses. This result, combined with an elaborate failed attempt to resynthesize the lineage, suggests that vertebrate gynogens such as the Amazon molly are not rare because they are at a disadvantage to their sexual counterparts, but because the genomic conditions under which they arise are rare. Organisms that apparently combine the disadvantages of both sexuality and asexuality remain difficult to understand from both an ecological and an evolutionary perspective, and Stöck et al. (2010) highlight several outstanding important questions. Nonetheless, given that we now have a better knowledge of the origin and history of this unique 'species', this should allow researchers to better understand how these frozen F1's can persist amidst the masterpiece of nature.

  12. 结球甘蓝新品种浙甘85的选育%A New Cabbage F1 Hybrid-'Zhegan 85'

    Institute of Scientific and Technical Information of China (English)

    钟新民; 李必元; 王五宏; 岳智臣

    2011-01-01

    浙甘85是以S04-C01为母本,以S05-SXGL为父本配制而成的甘蓝一代杂种.中晚熟,从定植到始收85 d(天)左右;叶球紧实,圆球形,不易裂球,耐贮运,球高20 cm左右,横径20~22 cm,中心柱长6~8 cm,单球质量2.4 kg左右;抗TuMV和黑腐病,一般每667 m产量5 000 kg左右,适宜长江流域及华南、华北、西南地区秋季种植.%‘Zhegan 85’ is a new cabbage F1 hybrid bred by crossing S04-G01 as female parent and S05-SXGL as male parent. It is a mid-late maturing variety, taking about 85 days from transplanting to harvest. The round head is compact and tough in structure which makes it a long shelf life. Its leaf head is 20 cm in height, 20-22 cm in diameter, with a short core of 6-8 cm in length. The head weight is about 2.4 kg. It has resistance to TuMV and black rot. Its yield is about 75 t · hm-2. Consequently, it is suitable to be cultivated in the Yangtze River valley, north China, south China, and southwest China.

  13. A New Chinese Cabbage F1 Hybrid -'Liaobai No.19'%大白菜新品种辽白19号的选育

    Institute of Scientific and Technical Information of China (English)

    吴海东; 王鑫; 吕艳玲; 王丽丽

    2011-01-01

    ‘Liaobai No.19’ is a new Chinese cabbage F1 hybrid developed by crossing genetic male sterility line Jiaobai No.1 A with selfing line Xinxiang 903-S10-1.Its growing period is about 82 days.The plant is 46.2 cm in height, 64.3 cm in width.Its leaf head is 37.5 cm in height, 17 cm in diameter.The average plant weight is 4.5 kg and the net product ratio is 87.78 %.It is resistant to TMV and downy mildew and soft rot.Its output is about 139.5 t · hm-2.It is suitable to be cultivated in Jilin, Liaoning,Heilongjiang Provinces and Inner Mogolian Autonomous Region, etc.%辽白19号是由细胞核基因雄性不育系胶白1号A和自交系新乡903-S10-1配制而成的筒形大白菜一代杂种.生育期82 d(天)左右,株高46.2 cm,开展度643 cm,球高37.5 cm,横径17.0 cm.单株叶球质量4.5 kg左右,净菜率87.78%,商品性好.生长势强,抗软腐病、霜霉病和病毒病,每667 m2产量9300kg左右.适宜吉林、辽宁、内蒙古、黑龙江等地栽培.

  14. A New Cucumber F1 Hybrid -'Jinyou No.4'%耐热黄瓜新品种津优4号的选育

    Institute of Scientific and Technical Information of China (English)

    马德华; 霍振荣; 李淑菊; 庞金安

    2001-01-01

    Jinyou No.4 is a new cucumber F1 hybrid with toleran ce to heat,high resistance to downy mildew,powdery mildew and Fusarium wilt. I t is suitable for open cultivation from spring to autumn. Its plant grows vigoro usly,and it yields 75t*hm-2 in spring. Average fruit is about 30cm length and 180g weight. Fruits are dark green,sweet and crispy,wit h white spine and have good quality and high marketable percentage.%津优4号的亲本之一P17是地方品种金早生突变株的多代自交后代,另一亲本T 55是利用自交系89-12的田间自然杂交株经多代自交选育的自交系。该一代杂种耐热,在夏季34~36℃高温下可正常发育。对枯萎病、霜霉病和白粉病的抗性强,春露地栽培产量可达75t*hm-2,夏秋栽培产量为35~40t*hm-2。果实棒状,长30cm左右,单瓜质量180g左右。商品性好,瓜色深绿,有光泽;瘤显著,密生白刺;果肉绿白色,质脆,味甜,品质优。适合华北地区春露地、秋露地和越夏栽培。

  15. 绿宝石西葫芦的选育%A New Summer Squash F1 Hybrid — ‘Lubaoshi’

    Institute of Scientific and Technical Information of China (English)

    刘宜生; 王长林; 王迎杰; 温凤英

    2001-01-01

    ‘Lubaoshi’is a new summer squash F1 hybrid developed by crossing mosaic leaf summer squash inbred line 94011 as female parent with dark green skin summer squash inbred line 94001 as male parent. It has vigorous plants,and long rhabdoid fruits with dark green skin. Its yield under protected cultivation is about 75 t*hm-2 and is 14.5 %~28.3 % higher than that of ‘Zaoqingyidai’. It has been extended to Beijing,Liaoning,Shandong,Ningxia,Jiangshu etc..%绿宝石西葫芦是以从北京地区搜集的花叶西葫芦的分离后代中选出的稳定自交系94011为母本、以从荷兰引入的深绿皮品种的分离后代中选出的稳定自交系94001为父本配制而成的早熟一代杂种。属矮生类型,生长势较强,果形长棒形,皮色深绿,总产量比早青一代西葫芦增产14.5 %~28.3 %,一般保护地栽培产量为5 000 kg*(667 m2)-1左右。已在北京、辽宁、河北、山东、宁夏、江苏、安徽、广西、贵州、云南等地推广种植。

  16. 贵妃鸡与麒麟鸡杂交F1代血液生理生化指标的测定%Measurement of Blood Physiological and Biochemical of hybrid F1 of Princess Chicken and Frizzle Chicken

    Institute of Scientific and Technical Information of China (English)

    李乃宾; 杨芬霞; 杜炳旺; 陈洁波; 陶林

    2014-01-01

    为给贵妃鸡(♂)与麒麟鸡(♀)杂交F1代的人工饲养繁殖、健康状况检测以及经济开发等提供科学的参考数据和理论依据,试验对12周龄贵妃鸡与麒麟鸡杂交F1代进行血液生理生化指标测定与分析。结果表明:①血液生理指标:杂交公鸡红细胞计数(RBC)、血红蛋白浓度(HGB)、红细胞压积(HCT)在数值上极显著高于母鸡(P<0.01),白血球计数(WBC)、平均红细胞体积(MCV)显著高于母鸡(P<0.05);②血液生化指标:杂交公鸡的甘油三酯(TG)、尿酸(UA)指标在数值上显著高于母鸡(P<0.05),磷酸肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)指标在数值上显著低于母鸡(P<0.05)。杂交F1代的血液生理生化指标具有相对稳定性,这一结果能为贵妃鸡(♂)与麒麟鸡(♀)杂交F1代疾病诊断、治疗和饲养管理提供参考。%In order to provide scientific reference and theoretical basis for breeding, health testing and economic development of hybrid F1 of Princess Chicken and Frizzle Chicken, the blood physiological and biochemical indexes of 12-week-old F1 Hybrid Offspring of Princess Chicken(♂)and Frizzle Chicken (♀)were measured.The results showed that①The blood physiological indexes:12 weeks of F1 generation of hybrid cock red blood cell count (RBC), hemoglobin concentration (HGB)and RBC deposited (HCT) were very significantly higher than the hens (P<0.01), average white blood cell count (WBC) and red blood cell volume (MCV) were significantly higher than the hens (P<0.05);②The blo-od biochemical indexes:12 weeks of F1 generation of hybrid cock triglyceride (TG)and uric acid (UA) index were significantly higher than the hens (P<0.05), creatine phosphate kinase (CK)and creatine kinase isoenzyme (CK-MB) index were significantly lower than the henl (P<0.05).The blood biochemical indicators of 12-week-old hybrid chickens were relatively stable,this conclusion provided a certain theoretical

  17. Study on Competitive Advantage of Glyphosate-resistant Cotton Hybrid F1%抗草甘膦棉花杂交种F1代竞争优势研究

    Institute of Scientific and Technical Information of China (English)

    刘亚平; 田绍仁; 杨兆光; 肖远龙; 涂祈钧

    2011-01-01

    [目的]筛选具有高产、优质、抗草甘膦,综合性状优良的棉花杂交种.[方法]以抗草甘膦棉花品种(系)作父本/母本,与常规优良品种(系)组配14个杂交组合,分析测定各杂交组合在产量、产量构成因子、抗虫和抗草甘膦方面的竞争优势表现.[结果]14个杂交组合生育期较对照赣棉杂1号提前,高抗草甘膦,其中组合① 、⑤ 、⑨ 、⑩ 、[11] 抗棉铃虫;组合②、⑤、⑥、⑩产量构成因子协调,籽、皮棉产量高于对照赣棉杂1号;组合① 、②、③ 、⑤ 、[14] 棉纤维品质较好.[结论]综合抗性、结铃进度、农艺性状、产量和纤维品质等性状,最终筛选出②、⑤、⑥ 、⑩ 等4个高产、优质、抗草甘膦、综合性状优良的杂交组合.%[ Objective ] The hybrid glyphosate-resistant cotton varieties with high yield, fine quality and good comprehensive properties were selected. [ Method] Glyphosate-resistant cotton was hybridized with conventional fine varieties to make 14 hybrid combinations whose yield,yield component factors, pest resistance and glyphosate resistance were analyzed and determined. [ Result ] The growth periods of these 14 hybrid combinations were all earlier than Ganmianza 1 ( CK2 ). They also showed high resistance to glyphosate, of which combinations number with ①、⑤、⑨、⑩ had resistance to cotton bollworm; combinations number with ②、⑤、⑥、⑩ had coordinated yield factors and higher seed plus lint yield than Ganzamian 1 (CK); combinations number with ①、② 、③、⑤、[14] had better lint quality. [ Conclusion] Based on characteristics of comprehensive resistance, bolling process, agronomic traits, yield and fiber quality, combinations numbered with ②、⑤、⑥、⑩ which had high yield, fine quality, glyphosate resistance and good comprehensive properties were selected.

  18. DNA barcoding identifies a cosmopolitan diet in the ocean sunfish

    Science.gov (United States)

    Sousa, Lara L.; Xavier, Raquel; Costa, Vânia; Humphries, Nicolas E.; Trueman, Clive; Rosa, Rui; Sims, David W.; Queiroz, Nuno

    2016-01-01

    The ocean sunfish (Mola mola) is the world’s heaviest bony fish reaching a body mass of up to 2.3 tonnes. However, the prey M. mola consumes to fuel this prodigious growth remains poorly known. Sunfish were thought to be obligate gelatinous plankton feeders, but recent studies suggest a more generalist diet. In this study, through molecular barcoding and for the first time, the diet of sunfish in the north-east Atlantic Ocean was characterised. Overall, DNA from the diet content of 57 individuals was successfully amplified, identifying 41 different prey items. Sunfish fed mainly on crustaceans and teleosts, with cnidarians comprising only 16% of the consumed prey. Although no adult fishes were sampled, we found evidence for an ontogenetic shift in the diet, with smaller individuals feeding mainly on small crustaceans and teleost fish, whereas the diet of larger fish included more cnidarian species. Our results confirm that smaller sunfish feed predominantly on benthic and on coastal pelagic species, whereas larger fish depend on pelagic prey. Therefore, sunfish is a generalist predator with a greater diversity of links in coastal food webs than previously realised. Its removal as fisheries’ bycatch may have wider reaching ecological consequences, potentially disrupting coastal trophic interactions. PMID:27373803

  19. DNA barcoding identifies a cosmopolitan diet in the ocean sunfish

    Science.gov (United States)

    Sousa, Lara L.; Xavier, Raquel; Costa, Vânia; Humphries, Nicolas E.; Trueman, Clive; Rosa, Rui; Sims, David W.; Queiroz, Nuno

    2016-07-01

    The ocean sunfish (Mola mola) is the world’s heaviest bony fish reaching a body mass of up to 2.3 tonnes. However, the prey M. mola consumes to fuel this prodigious growth remains poorly known. Sunfish were thought to be obligate gelatinous plankton feeders, but recent studies suggest a more generalist diet. In this study, through molecular barcoding and for the first time, the diet of sunfish in the north-east Atlantic Ocean was characterised. Overall, DNA from the diet content of 57 individuals was successfully amplified, identifying 41 different prey items. Sunfish fed mainly on crustaceans and teleosts, with cnidarians comprising only 16% of the consumed prey. Although no adult fishes were sampled, we found evidence for an ontogenetic shift in the diet, with smaller individuals feeding mainly on small crustaceans and teleost fish, whereas the diet of larger fish included more cnidarian species. Our results confirm that smaller sunfish feed predominantly on benthic and on coastal pelagic species, whereas larger fish depend on pelagic prey. Therefore, sunfish is a generalist predator with a greater diversity of links in coastal food webs than previously realised. Its removal as fisheries’ bycatch may have wider reaching ecological consequences, potentially disrupting coastal trophic interactions.

  20. DNA barcoding identifies a cosmopolitan diet in the ocean sunfish.

    Science.gov (United States)

    Sousa, Lara L; Xavier, Raquel; Costa, Vânia; Humphries, Nicolas E; Trueman, Clive; Rosa, Rui; Sims, David W; Queiroz, Nuno

    2016-07-04

    The ocean sunfish (Mola mola) is the world's heaviest bony fish reaching a body mass of up to 2.3 tonnes. However, the prey M. mola consumes to fuel this prodigious growth remains poorly known. Sunfish were thought to be obligate gelatinous plankton feeders, but recent studies suggest a more generalist diet. In this study, through molecular barcoding and for the first time, the diet of sunfish in the north-east Atlantic Ocean was characterised. Overall, DNA from the diet content of 57 individuals was successfully amplified, identifying 41 different prey items. Sunfish fed mainly on crustaceans and teleosts, with cnidarians comprising only 16% of the consumed prey. Although no adult fishes were sampled, we found evidence for an ontogenetic shift in the diet, with smaller individuals feeding mainly on small crustaceans and teleost fish, whereas the diet of larger fish included more cnidarian species. Our results confirm that smaller sunfish feed predominantly on benthic and on coastal pelagic species, whereas larger fish depend on pelagic prey. Therefore, sunfish is a generalist predator with a greater diversity of links in coastal food webs than previously realised. Its removal as fisheries' bycatch may have wider reaching ecological consequences, potentially disrupting coastal trophic interactions.

  1. 樱桃番茄新品种红艳艳1号的选育%A New Cherry Tomato F1 Hybrid, Bright Red 1

    Institute of Scientific and Technical Information of China (English)

    林涛; 李锦泉; 黄青峰; 陈朝文; 林金秀

    2011-01-01

    Bright Red 1 (CHT07×CHT81) is a new, early maturing cherry tomato F1 hybrid. Its parents were the offspring selected from Japanese and Taiwan commercial varieties. In the spring of 2004, 6 combinations were obtained by crossing 3 cherry tomatoes of the indeterminate growth habit type, CHT01, CHT02 and CHT07, as female, and two of the determinate growth habit type, CHT56 and CHT81, as male parent. Their heterosis and comprehensive characteristics were studied in the autumn and winter of 2004. Three cultivation seasons in the two-year (2005 - 2006) followed by a multi-year (2006 - 2008) comparison tests and a production demonstration were conducted. As a result, a new indeterminate growth habit type tomato variety with strong growth potential and high resistances to ToMV and leaf mould was selected. The plant produced mature fruits red in color, oblong in shape, crisp in texture, sweet in taste and weighed 16 -18 grams per fruit. It had desirable commodity characteristics for storage and transportation. It's the average yield of the plant was 52 500 - 60 000 kg · Hm-2. The variety was certified as a new cultivars by the Provincial Crop Variety Approval Committee in 2011.%红艳艳1号(CHT07×CHT81)的亲本分别从日本和台湾的商业F1品种后代分离选出;2004年春季选用3个无限生长型的樱桃番茄自交系CHT01、CHT02和CHT07作母本,2个有限生长型的自交系CHT56和CHT81作父本,配制组合6个,秋冬季进行组合比较试验,调查了解各组合的杂种优势和综合性状;红艳艳1号是经过2005~2006年2年3季的品种比较试验,2006~2008年的多年多点试验和生产试验示范,选育出的早熟樱桃番茄杂种一代.该品种属无限生长型,生长势强,高抗ToMV和叶霉病;果实长椭圆形,成熟果红色,单果重16~18 g,口感风味脆甜,耐贮运,一般产量为52 500~60000 kg· hm-2;2011年通过福建省农作物品种审定委员会认定.

  2. A New Pink Tomato F1 Hybrid-‘Zhengfan 1037’%粉果番茄新品种郑番1037的选育

    Institute of Scientific and Technical Information of China (English)

    应芳卿; 黄文; 李自娟; 黄晓燕

    2015-01-01

    郑番1037是以自交系10A9青茎为母本、10Y153-混1为父本配制而成的早熟番茄一代杂种,无限生长类型,植株生长势强;叶量中等,叶色深绿、平展;始花节位为第6节,花序间隔3片叶,每花序着花5~6朵;果实大小均匀,连续结果能力强,幼果微具绿果肩,成熟果粉红色,圆形;平均单果质量150 g,可溶性固形物含量4.9%,平均每667 m2产量6230 kg;田间对TMV和叶霉病的抗性强于对照东农712。适合春秋保护地和露地种植。%‘Zhengfan 1037’is a new tomato F1 hybrid developed by crossing inbred line 10A9 qingjing stem as female parent and 10Y153-hun 1 as male parent. It is of early maturity,and belongs to unlimited growth type. It grows vigorously,and has mideum leaf number. Its leaf is dark green in color and flat in shape. The first flower sets at the 6th node,and the inflorescence interval is 3 leaves. Each inflorescence has 5-6 flowers. It has continues fruit setting ability. The tender fruit has light green shoulder,the mature fruit is of round shape with pink color. Its single fruit weight is 150 g with 4.9%soluble solid content. It can yield 93.45 t·hm-2. It has higher resistant to TMV and leaf mold than the control‘Dongnong 712’in field. It is suitable to be planted in protected and open fields in spring and autumn.

  3. A New Tomato F1 Hybrid-‘Weikefen No.3’%番茄新品种潍科粉3号的选育

    Institute of Scientific and Technical Information of China (English)

    刘建民; 吕金浮; 乔宁; 刘晓明; 李美芹; 王兴翠

    2014-01-01

    潍科粉3号是以H10A为母本,以J10211为父本杂交选育而成的粉果番茄一代杂种。早熟,由定植到第1穗果成熟需75 d(天)左右,植株长势强,无限生长类型,连续坐果能力强,始花节位在第6~7节;果实略高圆形,着色均匀,亮粉色,果实味浓爽口,风味佳,VC含量高达256 mg·kg-1;硬度高,萼平展,商品性好,单果质量230~250 g,每667 m2产量13000 kg以上;对番茄黄化曲叶病、疫病、叶霉病的抗性较对照梦之粉强。适于温室和大棚早春、秋延后及越冬栽培。%‘Weikefen No.3’is a new tomato F1 hybrid developed by crossing H10A as female parent and J10211 as male parent. It is early maturing. It takes only about 75 days from planting to fruit ripening. Its plant grows vigorously, and belongs to infinite growth type. It has continuous fruit setting ability. The first inflorescence sits at 6 to 7 nodes of the main vine. Its fruit is tall and round in shape with even pink color. The single fruit weight is 230-250 g. It can yield over195 t·hm-2. Its fruit has strong rigidity,spreading sepals,super commercial performance,and good taste. Its VC content is 256 mg·kg-1. It has stronger resistance to tomato yellow leaf curl virus,blight,leaf mold than‘Mengzhifen’. It is suitable to be cultivated in greenhouse in early spring,late autumn and over winter.

  4. Breeding of Dida, a New Tomato F1 Hybrid Resistant to TY and RKN%多抗番茄新品种迪达的选育

    Institute of Scientific and Technical Information of China (English)

    张保才; 张秀荣; 化娟莉; 张延安

    2016-01-01

    Dida is an early-medium maturity tomato F1 hybrid developed by crossing M07-18 as female parent and M10-155 as male parent. It is unlimited growth type, and grows vigorously. Its first flower sets on 9th-10th node, with every three nodes setting an inflorescence. The fruit setting ability of this cultivar is strong, and the fruit is round and flat with smooth surface. The immature fruit is bluish white and the matured fruit is red. The single fruit weight is 220-350 g, and the fruit hardness is better. Dida is tolerant to storage and transportation, and resistant to TY, root knot nematode (RKN) and gray leaf spot. Its yields are 6 800-7 500 kg/667 m2 under protected cultivation in late autumn, and above 8 000 kg/667 m2 under greenhouse cultivation, so it is suitable to be planted in protected field.%迪达是以自交系M07-18为母本、自交系M10-155为父本配制而成的早中熟红果番茄一代杂种。植株无限生长类型,长势强,始花节位9~10节,花穗间隔3节,坐果能力强,果实圆形略扁,无果肩,果面光滑,青果青白色,熟果红色,单果质量220~250 g,硬度佳,货架期长。田间抗番茄黄化曲叶病毒病、南方根结线虫病、灰叶斑病;秋延后保护地栽培667 m2产量6800~7500 kg,温室栽培产量8000 kg以上,适宜保护地栽培。

  5. A New Tomato F1 Hybrid-‘Hongrui No.1’%番茄新品种红瑞1号的选育

    Institute of Scientific and Technical Information of China (English)

    陈晓军; 吴多志; 杨国帅

    2015-01-01

    ‘Hongrui No.1’ is a new tomato F1 Hybrid developed by crossing RZHT257 as female parent and RZHT006 as male parent. This variety is of unlimited growth type. It is of early maturity. It has strong growth vigor. Its leaf is green in color and big in size. The space between inflorescence is 2-3 leaf blades. Its fruit is of round shape. The matured fruit is red in color with hard pulp and has no green shoulder. It has multiple ventricular and good commercial characteristics. The average single fruit weight is 182.7 g. Its shelf life is 8-10 days. The soluble solid content is 5.7%. Its resistance to powdery mildew,leaf mold and early blight is stronger than that of the contrast ‘Zhongshu No. 4’. It is suitable for open and protected field cultivation in Gansu,Shandong,Hebei Provinces. Its average yield is 85.9 t.hm-2.%红瑞1号是以RZHT257为母本、以RZHT006为父本配制而成的番茄一代杂种。无限生长类型,早熟,植株生长势强,叶色绿,大叶,7~8片真叶着生第1序花,花序间隔2~3叶;果实圆形,成熟果红色无绿肩,果实硬,多心室,商品性好,商品果率高;平均单果质量182.7 g,货架期8~10 d(天),可溶性固形物含量5.7%;红瑞1号对白粉病、叶霉病和早疫病的抗性强于对照中蔬4号,平均每667 m2产量5726.9 kg,适宜甘肃、山东、河北等地露地及保护地栽培。

  6. 早熟秋甘蓝新品种达光的选育%A New Autumn Cabbage F1 Hybrid-‘DaGuang’

    Institute of Scientific and Technical Information of China (English)

    张保才; 化娟莉; 张延安

    2014-01-01

    ‘Daguang’ is a new autumn cabbage F1 hybrid developed by crossing self-incompatible line 98-19 as female parent and self-compatible line 99-36 as male parent.It is an early-maturing variety.It takes about 72 days from field planting to harvest.The plant grows vigorously.Its expansion degree is 49-52 cm. It has 12-14 outer leave blades,and the leaf is greyish green in color.There is a lot of wax powder on the leaf surface.The leafy head is oblate,green and compact.The compactness degree is 0.62.The leafy head is 19.8 cm in transverse diameter,11.3 cm in height.The central core is 5.9 cm in length.The single leafy head weights 1.5 kg.It has crisp texture and slightly sweet taste.It is tolerant to head splitting,and resistant to TuMV(Turnip mosaic virus)and black rot.Its average yield is about 67.5 t·hm-2.It is suitable to be cultivated in autumn open fields at north,central,and southwest part of China.%达光是以自交不亲和系98-19为母本,以自交系99-36为父本配制而成的早熟秋甘蓝一代杂种。早熟,定植至收获72d(天)左右;植株生长势强,株型平铺,开展度49~52cm,外叶12~14片,叶色灰绿,叶面蜡粉多;叶球扁圆形、色绿,紧实度0.62,球高11.3cm,横径19.8cm,中心柱长5.9cm,单球质量1.5kg,质地脆嫩,味微甜,耐裂球;抗芜菁花叶病毒病(TuMV)和黑腐病;一般每667m2产量4500kg左右,适宜华北、华中及西南地区秋季露地种植。

  7. 砍瓜×广西蜜本南瓜的杂种鉴定%Identification of Hybrid F1 of Cucurbita kangua Li. × Cucurbita moschata Duch.

    Institute of Scientific and Technical Information of China (English)

    李晓林; 韦静宜; 叶绍华

    2007-01-01

    采用聚丙烯酰胺凝胶垂直平板电泳法对砍瓜(母本)、广西蜜本南瓜(父本)以及杂种F1代的POD与Est同工酶进行测定,结果表明,父母本的两种同工酶均有明显差异,F1代两种同工酶谱表现出双亲的互补性,F1代Est同工酶出现杂种酶带Rf=0.816.证明POD和Est同工酶是可以用来鉴定南瓜品种及其杂种.气孔密度和形态也反应出双亲与F1代的差异,F1代气孔密度介于双亲之间,气孔形态偏向于父本.因此,气孔形态特征也可用来鉴定双亲和杂种.F1代瓜皮、瓜肉颜色和品质更接近父本.

  8. 大梅组合中亲子代甲基化差异及其与生产性状的关系%Differences of Cytosine Methylation in Parental Lines and Hybrid F1 of Large White × Meishan and Their Effects on F1 Performance

    Institute of Scientific and Technical Information of China (English)

    蒋曹德; 邓昌彦; 熊远著

    2005-01-01

    In order to probe the effect of methylation on heterosis, methylation-sensitive AP-PCR technique was adopted to amplify pig genome DNA with 40 single arbitrary primers. The material involved parental lines and hybrid F1 of Large White × Meishan.Nineteen cdifferentially methylated sites with Rsa Ⅰ +Hpa Ⅱ digestion and 14 differentially methylated sites with Rsa Ⅰ +Msp Ⅰ digestion between parental lines and the hybrid were found. All fragments detected in this study were grouped into four classes: (1) the same level of methylation in both parental lines and the hybrid; (2) the same level of methylation in one parent and the hybrid; (3) an increased level of methylation in the hybrid compared to the parents, and (4) a decreased level of methylation in the hybrid. Five sites had significant effects on 7 traits (P <0.05). Sequence analysis showed that three sequences had their match in GenBank with high identity (greater than 87%) and two sequences had no match in the database. G.C percent of three sequences was over 50, and observed/expected CpG of all sequences was above 0.6. Furthermore, one sequences contained G/C boxes. This study has demonstrated that sites in CpG islands within a gene promoter region were differentially methylated in the hybrid compared to parental lines; methylated sites exerted differentially on F1 performance, showing heterosis could benefit from expression or repression of some genes.%为了探讨DNA甲基化对杂种优势的影响,采用了甲基化敏感随机扩增PCR技术(methylation-sensitiveAP-PCR,MS-AP-PCR),利用40条随机引物对大梅组合亲子代基因组DNA进行了扩增.结果:在RsaⅠ+HpaⅡ酶切扩增片段中,有19个位点在亲子代出现差异;在RsaⅠ+HpaⅡ酶切扩增片段中,有14个位点在亲子代出现差异.亲子代间甲基化状况可归纳为4种类型:(1)亲子代甲基化水平相同;(2)单亲与F1代甲基化水平相同;(3)同亲代相比,F1代发生甲基化;(4)同亲代相比,F1

  9. Chromosomal Constitution and Karyotype of Intergeneric F1 Hybrids Between Saccharum spp.and Erianthus fulvus Based on GISH%基于GISH的甘蔗与蔗茅属间杂交F1后代染色体组成及核型分析

    Institute of Scientific and Technical Information of China (English)

    王先宏; 杨清辉; 李富生; 何丽莲; 何顺长

    2011-01-01

    [Objective] The objective of current experiment was to study the chromosomal constitution and karyotype of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and Erianthusfulvus. [Method] Both monocolor and bicolor GISH (Genomic in situ hybridization) were performed using digoxigenin-labeled E. fulvus DNA and biotin-labeled female parental DNA as probes, the karyotypes of 3 hybrids were analyzed based on the result of GISHs. [ Result ] The results of GISHs revealed the transmission of 7 to 10 E. fulvus chromosomes into progenies. The chromosomes of E. fulvus were broken because of the female parents prevented alien genetic materials from introgressing during distant hybridization and resulted in elimination and fragmentation of E. fulvus chromosomes. The GISH result on nuclear of progeny showed the chromatins came from male and female parent are not intermixed and stays at different regions. The karyotype formulas of 3 hybrids were 2n=92=80m (4SAT)+12sm.2n=88=82m+6sm and 2n=88=78m(2SAT)+10sm, and their karyotypes were 2C, 2C and 2B, respectively. [Conclusion]Chromosomal constitution of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and E. fulvus were n+n and 2n+n. The results of karpotype analysis showed that the chromosomes of these three progenies are primitive.%[目的]探讨甘蔗(Saccharum spp.)与蔗茅(Erianthus fulvus)杂交F1材料的染色体组成及核型差异.[方法]以甘蔗与蔗茅间杂交所获得的16份F1材料为研究对象进行单色及双色基因组原位杂交(genomic in situ hybridization,GISH)研究,在GISH的基础上对3份材料的中期染色体进行核型分析.[结果]GISH的研究结果表明,子代材料中具有7-10条不等的父本染色体; 由于母本遗传物质对父本遗传物质的排斥作用引起的染色体消减及片段化导致父本染色体在于代中有断裂的现象;研究结果表明,在间期核中亲本染色质

  10. 西葫芦新品种青葫1号的选育%A New Summer Squash F1 Hybrid -‘Qinghu No.1’

    Institute of Scientific and Technical Information of China (English)

    侯全刚; 邵登魁; 李江; 李全辉; 张广楠

    2016-01-01

    ‘Qinghu No.1’is a new summer squash F1 hybrid developed by crossing inbred line‘0452-086’ as female parent and inbred line‘0921-033’as male parent.The plant grows vigorously with short nods.The first female flower sets on the 5-6 th nodes.The fruit is of straight,long stick shape with dark green peel.The average verticle diameter is 28.75 cm and transverse diameter is 7.27 cm.The single fruit weight is about 670 g. It has continuous fruit setting ability,and good commodity quality.Its average yield can be over 61.5 t·hm-2,the highest one can reach over 79.5 t·hm-2.It takes about 50 days from sowing to harvesting in Xining region.The whole growth duration is about 120 days.In fields,its resistance to powdery mildew is stronger than that of the contrast‘Zaoqingyidai’.It is suitable for planting in greenhouse the whole year round in Qinghai Province and in opening field at regions under 2 900 m altitude over summer.%青葫1号是以优良自交系0452-086为母本,以0921-033为父本配制而成的早熟西葫芦一代杂种。矮蔓生,第1雌花节位为第5~6节,瓜条顺直,长棒形,平均纵径28.75 cm,横径7.27 cm,皮色墨绿,单瓜质量670 g 左右,连续结瓜能力强,成瓜率高,商品性好。每667 m2平均产量达4100 kg 以上,高产田每667 m2产量可达5300 kg 以上。西宁地区播种到采收50 d(天)左右,采收期比对照早青一代早10 d(天)左右,全生育期120 d(天)左右。田间对白粉病的抗性强于对照早青一代。适宜在青海省各地温室周年栽培,及海拔2900 m 以下地区露地越夏栽培。

  11. Different Feeding Levels and Weight Gain Test of Yunnan F1 Hybrid of Murrah and Local Buffalo Calves%不同补饲水平摩本杂F1代奶水牛犊牛增重效果研究

    Institute of Scientific and Technical Information of China (English)

    白文顺; 毛华明; 李思银; 杨亮宇

    2012-01-01

    [目的]本试验旨在提高犊牛早期日增重的方法,进行本次试验。[方法]采用日龄、体型、体重均相近的1月龄以内的摩本杂F1代奶水牛犊牛14头,随机分成试验A组和试验B组,分别饲喂不同日粮(试验A组完全补饲精料补充料、试验B组补饲精料补充料与苜蓿颗粒各占50%),试验期为163d,试验结束后比较两组的日增重。[结果]补饲水平不同对云南摩本杂F1代奶水牛犊牛早期增重影响很大,试验A组增重效果明显好于试验B组。[结论]试验表明在云南摩本杂F1代奶水牛犊牛早期饲养过程中适当增加精料补充料的饲喂量,可以获得更高日增重。%[Objective]To explore the ways of improving early calf daily gain to provide a reference for the research of buffalo nutrition supply as well as the actual production. [Method]14 buffalo calves less than 1 month old with similar age, size and weight were randomly divided into group A and group B,which were fed different diets (group A completely feeding feed supplement,accounting for 50% of the group B supplementation feed test the daily gain of the early weight gain supplement with alfalfa particles). The trial period was 163 days. the two groups were compared. [Results]Different feeding level had for hybrid F1 buffalo calves, and group A was significantly better At the end of the great influence on than that of group B. [Conclusion]The tests showed that the appropriate increase in the amount of feed supplement could get higher daily gain for Yunnan F1 hybrid of Murrah and Local buffalo Calves in early feeding process.

  12. A Comparative Study on Salt Tolerance of Wild,Cultivated and their Hybrid F1 Tomato Seedlings Based on the Ion Homeostasis%基于离子稳态的野生与栽培番茄及其杂交F1的耐盐性差异

    Institute of Scientific and Technical Information of China (English)

    敖雁; 管永祥; 孙云贺; 彭益全; 吴昊; 乔燕; 郑青松

    2016-01-01

    An experiment was conducted to study effects of salinity,varying in level,50,100 or 200 mmol L-1 NaCl,on plant growth,ion absorption and distribution of wild tomato Solanum pimpinellifolium (WT),cultivated tomato(S. esculentum)(CT)and their hybrid F1 generation. Results showed that dry weight(DW)of the three tomato varieties decreased significantly under salt stress,either 50,100 or 200 mmol L-1NaCl,displaying a descending order of CT>WT> F1. With rising salt stress,Na+ content increased,while K+ content decreased significantly in all the tomato plants. Besides,under salt stress,K+/Na+ decreased significantly in all the tomato plants and the decreases in the plants,regardless of variety, were similar in extent. With rising salt stress,the roots of the plants of either variety enhanced their K+ or Na+ selectivity ratio(SK,Na),but the enhancement varied slightly in extent with variety,showing an order of WT> F1>CT. However,salt stress decreased stem SK,Na in all the tomato plants by a similar degree. But with rising salt stress,the drop in CT was much bigger than that in F1. Salt stress increased leaf SK,Na of all the tomato plants,but with rising salt stress,leaf SK,Na declined in WT,while it kept increasing gradually in CT and F1 and the rise in F1 was more significant than that in CT. To sum up,the F1 generation is similar to its wild parents in maintaining ion balance,especially in their leaves,which makes F1 generation better in growth and higher in salt tolerance than its parents.%以野生番茄-醋栗番茄D4-101(Solanum pimpinellifolium)(WT)、自交系番茄栽培种7818D(S. esculentum)(CT)及二者杂交产生的F1代品系为材料,探讨50、100及200 mmol L-1 NaCl胁迫处理对番茄生物量和离子吸收分配的影响。结果表明:盐胁迫下,植株干重的降幅由大到小依次是:CT>WT>F1。随着盐胁迫程度加大,三个品种(系)Na+含量均增高,而K+含量显著降低。盐胁迫处理下三个

  13. Cytogenetic Analysis of Hybrid F1 of Common Wheat and Aegilops Venaicosa × Aegilops Cylindrica Amphidiploid%偏凸-柱穗山羊草双二倍体与普通小麦杂种F1的细胞遗传学分析

    Institute of Scientific and Technical Information of China (English)

    明东风; 王秀芹; 付琪; 赵庆玮; 赵逢涛; 王玉海

    2011-01-01

    [objective] The aim of this study is to investigate the chromosome pairing of F, from Aegilops ventricosa-Aegilops cylindrica amphip loid X common wheat. [ Method] Microsporogenesis and pollen development of YannongI5, SDAU18 and their hybrid F, were observed cyto logically by squash method. [Result] The results showed that microsporogenesis and pollen development of two parents including Yannongl5 and SDAU18 were basically normal, and their seed setting rate was also basically normal. However, the microsporogenesis of their hybrid F, was chaotic, linivalent and bivalent with high frequency of 10.11 and 18. 29 per cell respectively occurred in meiotic M I (metphase I ) of PMC (pollen mother cell). Nevertheless, only quite low frequency of multivalents occurred in meiotic PMC M I . The laggards at meiotic AI (anaphase I ) were 5 per cell on average. The number of micronucleoli in tetrad was 4.43 per cell averagely. The aborted pollen with differ ent frequency occurred at every stage of hybrid F, pollen development but mainly after single nucleus pollen stage. The fertile pollen rate of hybrid F, was only 31% at 3-nucleated stage. [Conclusion] This study provides cytogenetics basis for further analysis and utilization of the amphiploid.%[目的]研究偏凸-拄穗山羊草双二倍体与普通小麦杂种F1的染色体配对特点.[方法]利用压片法对普通小麦烟农15、SDAU18及其杂种F1的小孢子发生和花粉发育过程进行了细胞学观察.[结果]两亲本(烟农15和SDAU18)的小孢子发生和花粉发育基本正常,二者结实率也基本正常.F1小孢子发生过程紊乱,花粉母细胞减数分裂中期Ⅰ出现较高频率的单价体和二价体,分别为10.11和18.29个,而多价体出现的频率非常低;减数分裂后期Ⅰ,每个细胞落后染色体平均为5条;四分体期,每个四分体微核数平均为4.43个.在杂种F1花粉发育的每个时期都产生了不同频率的败育花粉,但败育主要发生在第1

  14. Laboratory and field studies on biotransformation enzymes in sunfish (Lepomis sp. )

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez, B.D.; Oikari, A.; Adams, S.M.; Esau, B.D.; Morrissey, C.M.; Petanen, T.I. (Oak Ridge National Lab., TN (USA))

    1988-09-01

    To cope with pollutants, organisms synthesize enzymes that detoxify organic compounds (mixed function oxidases or MFOs). A series of these enzymes are presently being employed as indicators of pollution at Oak Ridge National laboratory in the Y-12 Biological Monitoring and Abatement Program. The quantities and activities of several hepatic enzymes, as well as the liver somatic indices, were measured in redbreast sunfish, Leopomis auritus, sampled from several stations along a stream receiving large inputs of industrial and municipal effluents and a stream in a non-industrialized, rural drainage area. Fish collected close to the industrial and municipal outfalls exhibited high quantities and activities of these enzymes, as well as elevated somatic indices, compared to those from the rural stream and from other locations along the polluted stream. Laboratory experiments were conducted using Bluegill-green sunfish hybrid which were exposed to approximately 0.5 g Benzo(a)pyrene (BaP) per liter under 24 h static renewal conditions. The time course of induction for MFO activity was obtained. Induction of MFO activity occurred a week after BaP exposure.

  15. Rapidly Identifying Hybrids of Peanut (Arachis Hypogaea L.) Using SSR Molecular Markers%利用SSR快速鉴别花生杂交F1真伪

    Institute of Scientific and Technical Information of China (English)

    洪彦彬; 李少雄; 李杏瑜; 朱方何; 梁炫强

    2012-01-01

    In this research, we used six peanuts varieties as parents to make hybrid combinations for hybrid identification. Our practice showed that the authenticity of F1 hybrids would be easy validated by field agronomic investigation, if whose parents had visible and distinguishable phenotypic differences, otherwise it might be very difficult to identify the authenticity of F1 hybrids. Therefore, we established an SSR-PCR rapid detection system for peanut identification by modifying Thomson's one-step protocol to prepare DNA template, which was applied to identify the authenticity of peanut F, hybrids. The results showed that DNA templates extracted from Thomson's one-step protocol and modified Thomson's one-step protocol were able to generate sharp bands. Peanut DNA prepared by the modified protocol stored at 4℃ for almost a month was still fresh and working well, whereas peanut DNA prepared by Thomson's was completely degraded only staying a day at 4℃. The authenticity of F, hybrid combinations in this study were identified to be 38%~56%, the results further indicated that hybrid rates should be differences among different parents, even one pair parents for reciprocal cross. Therefore, we thought that using SSR markers to identify the authenticity of peanut hybrid might be great potential in future.%本研究,我们以6个花生品种为亲本配置杂交组合,田间调查发现亲本间形态表型差异大的杂交F1容易鉴别真伪,而形态表型差异小的则难于鉴别,甚至无法鉴别.为此,我们通过改良Thomson一步法制备DNA模板,建立了一套花生SSR-PCR快速检测体系,并在此基础上利用SSR鉴别花生杂交F1真伪.结果表明,采用Thomson一步法和改良后的一步法提取的DNA模版均能扩展出清晰条带,但改良后制备的DNA模板在4℃下可保存约1个月,未改良的仅能保存一天.利用SSR检测上述群体表明,F1真杂种率为38%~56%,不同亲本间杂交成功率存在差异,同一亲本

  16. Morphological recognition of artificial F1 hybrids between three common European cyprinid species:Rutilus rutilus,Blicca bjoerkna and Abramis brama%三种普通欧洲鲤科鱼类(拟鲤、粗鳞鳊和欧鳊)人工杂交F1代形态学观察

    Institute of Scientific and Technical Information of China (English)

    B. NZAU MATONDO; M. OVIDIO; P. PONCIN; P. VANDEWALLE; J.C. PHILIPPART

    2008-01-01

    对反交试验获得的18个月的拟鲤、粗鳞鳊和欧鳊F1代进行了鉴定.对不同于拟鲤、粗鳞鳊和欧鳊三种鱼的可量和可数形态特征分析表明,拟鲤×欧鳊和拟鲤×粗鳞鳊杂交种比粗鳞鳊×欧鳊杂交种与它们的双亲更容易区分.拟鲤杂交种间具有很多形态上的相似性,例如,上侧虹膜在拟鲤为红色,杂交种则介于双亲之间.在粗鳞鳊×欧鳊杂交种中也可以观察到居间类型.拟鲤×粗鳞鳊和拟鲤×欧鳊各自的杂交种可以通过眼睛的大小、较少的侧线鳞数和2行咽喉齿等特征相区分.粗鳞鳊×欧鳊杂交种与另外两个杂交种相比,具有更多的臀鳍分支鳍条数和一个中央具一黑线的虹斑.三个种进行杂交再获得的互交种通常不易区分.%The F1 hybrids of roach Rutilus rutilus, silver bream Blicca bjoerkna, and common bream Abramis brama from experimental reciprocal cross-breedings were identified at 18 months of age in relation to the parental species. The morphological analysis, including quantitative (plastic and meristic) and nonmetric features differing in the roach, the silver bream and the common bream, revealed that roach×common bream and roach×silver bream hybrids were easier to distinguish from their parents than silver bream×common bream hybrids. These roach hybrids had many morphological similarities such as the upper iris coloured in red as in the roach, and they were morphologically intermediate to the two parents. This contrasted with the silver bream×common bream hybrids, in which intermediate characteristics were also observed, but with some parental variants. Roach×silver bream hybrids were distinguishable from roach×common bream hybrids by its large eye, its lower scale numbers along the lateral line and its two rows of pharyngeal teeth. Silver bream×common bream hybrids, compared to the two other types of hybrids studied, had higher anal fin soft ray numbers and a clear eye iris with a median

  17. Ensaio de potência da alfaepoetina: Comparação de camundongos Swiss Webster, NIH, C57BL/6, BALB/c com o híbrido B6D2F1 /Potency assay of epoetin alpha: Comparison of Swiss Webster, NIH, C57BL/6, BALB/c mice with the hybrid B6D2F1

    Directory of Open Access Journals (Sweden)

    Igor Barbosa da Silva

    2013-08-01

    Full Text Available Neste estudo comparamos os resultados de ensaios de potência da alfaepoetina (EPOhr realizados com camundongos de diferentes colônias e linhagens (Swiss Webster, NIH, C57BL/6 e BALB/c com aqueles de ensaios conduzidos com o híbrido B6D2F1, o úni-co camundongo recomendado pela Farmacopeia Europeia (FE. Fêmeas de diferentes colônias e linhagens, pesando 16-18 gramas, receberam uma única dose de EPOhr por via subcutânea (30, 90 ou 270 UI/animal, 0,2 mL/camundongo. As potências biológi-cas de apresentações de 4.000 UI/mL da EPOhr foram avaliadas utilizando um material de referência de trabalho de alfaepoetina (3.773 UI/mL anteriormente testado junto ao padrão de referência internacional BRP (European Pharmacopeia Biological Refe-rence Preparation. Os resultados indicaram que camundongos das colônias e linhagens examinadas atingiram critérios estatísticos (FE para um ensaio válido de potência da eritropoietina e, portanto, podem ser considerados como alternativas ao uso do híbrido B6D2F1. Os ensaios com camundongos BALB/c, entretanto, foram os que produziram re-sultados mais semelhantes aos obtidos com os híbridos B6D2F1, em relação à contagem média de reticulócitos em resposta a 30, 90 e 270 UI/camundongo, e aos coefi cientes angulares (inclinação e lineares (intersecção da curva dose-resposta (curvas paralelas praticamente superpostas. --------------------------------------------------------------------------- In this study we compared the outcomes of epoetin alpha (rhEPO potency assays per-formed with Swiss Webster, NIH, C57BL/6 and BALB/c mice with those of the assay conducted with the B6D2F1 hybrid, the only mice recommended by the European Phar-macopoeia (EP. Female mice from different breeding stocks and strains, weighing 16-18 g, received a single subcutaneous injection of (30, 90 or 270 IU per mouse, 0.2 mL per mouse of rhEPO. Biological potencies 4000 IU/mL rhEPO pharmaceutical forms from di-fferent batches

  18. Characterization of beta2-glycoprotein I-dependent and -independent "antiphospholipid" antibodies from lupus-prone NZW/BXSB F1 hybrid male mice.

    Science.gov (United States)

    Thiagarajan, P; Le, A; Shapiro, S S

    1997-10-01

    Male (NZW x BXSB)F1 (W/BF1) mice develop a systemic lupus-like syndrome characterized by thrombocytopenia, coronary vascular disease, nephritis, and anticardiolipin antibodies. Three stable hybridoma cell lines secreting monoclonal anticardiolipin antibodies were developed from these mice by fusing their splenic lymphocytes with nonsecreting myeloma cell line, NS-1. Monoclonal antibody A1.17 reacted with cardiolipin in a beta2-Glycoprotein I-dependent manner. The epitope for this antibody consisted of beta2-glycoprotein I bound to cardiolipin or immobilized on plastic plates. Other anionic phospholipid-binding proteins, such as prothrombin or annexin V, had no significant effect in the reactivity of these antibodies. The specificity is similar to the autoimmune anticardiolipin antibodies described in patients with systemic lupus erythematosus and other infectious diseases. In contrast, monoclonal antibodies A1.72 and A1.84 reacted with cardiolipin in the absence of beta2-glycoprotein I. Beta2-glycoprotein I, either in the fluid phase or bound to cardiolipin, inhibited the binding of these antibodies. The specificity of the latter two antibodies was similar to that described in patients with syphilis and allied disorders. Both types of antibodies had lupus anticoagulant properties. Thus lupus-prone male (NZW x BXSB)F1 (W/BF1) mice develop both beta2-glycoprotein I-dependent and beta2-glycoprotein I-independent anticardiolipin antibodies.

  19. Construction, expression and characterization of a plasmid-encoded Na(+)-specific ATPase hybrid consisting of Propionigenium modestum F0-ATPase and Escherichia coli F1-ATPase.

    Science.gov (United States)

    Kaim, G; Dimroth, P

    1994-06-01

    The Escherichia coli strain DK8, a deletion mutant lacking the complete unc operon, was transformed with a plasmid containing the genes encoding the a, b, c, delta and part of the alpha subunit of the Na(+)-dependent ATPase of Propionigenium modestum and the genes encoding the alpha, gamma, beta and epsilon subunits of the H(+)-dependent E. coli ATPase. The transformants showed Na(+)-dependent growth on succinate as non-fermentable carbon source. The functionally expressed hybrid ATPase was activated 13-fold at pH 7.5 by the addition of Na+ and inhibited by 1,3-dicyclohexylcarbodiimide, azide and tributyltin chloride. At pH 7.5 and pH 9.0, the hybrid enzyme was protected from inhibition by 1,3-dicyclohexylcarbodiimide in the presence of 50 mM NaCl and 5 mM NaCl, respectively. The hybrid ATPase was reconstituted into proteoliposomes and catalyzed the transport of Na+ upon ATP addition. ATP-dependent fluorescence quenching of 9-amino-6-chloro-2-methoxyacridine proved that the ATPase hybrid was able to pump protons in the absence of Na+. Furthermore, ATP synthesis could be measured under conditions where a valinomycin-mediated K+ diffusion potential (delta psi) and a Na+ concentration gradient (delta p Na+) were imposed.

  20. Influence of strain of sire on channel x blue F1 hybrid catfish embryo production and fingerling performance under commercial farm conditions.

    Science.gov (United States)

    Channel x blue hybrid catfish is presently the desired aquaculture species in US farm-raised catfish industry. Even though production and performance has improved over the last two decades, several production and performance parameters needs to be improved to maximize the genetic potential of this ...

  1. 珍禽贵妃鸡与麒麟鸡杂交F1代肉用性能测定%Determination of the Meat Performance of the Hybrids from Princess Chicken and Frizzle Chicken

    Institute of Scientific and Technical Information of China (English)

    李乃宾; 杨芬霞; 杜炳旺; 陶林; 陈洁波; 李东华; 叶伟庆; 朱梓京

    2014-01-01

    In order to enhance the meat development value of Princess Chicken,the slaughter performanceand meat quality of 12-week-old Princess Chicken (♂), Frizzle Chicken (♀) and hybrid offspringwere mea-sured and compared. The results showed that the average weight of male (1236.67g) and female (1063.00g) of 12-week-old hybrid offspring was significantly higher than those of male parents-Princess Chicken (male 1025.67g, female704.67g)(P<0.05).The average slaughter rate of hybrid chickens was 90.22% and the average eviscerated ratio was 61.67%,so the index had reached the requirement of high quality chic-ken. At the same time, the Important economic characters and meat quality index of hybrid offspring, su-ch as body weight, carcass weight, eviscerated weight, leg muscle weight and chest muscle weight, show-ed good heterosis. Hybrid generation abundantly develop advantages of the parents, so we should make f-ull use of their respective advantages to deeper research and meet the demand of the market.%为了提高贵妃鸡的肉用开发价值,本试验以贵妃鸡为父本、麒麟鸡为母本进行杂交探索,对12周龄杂交F1代进行屠宰性能和肉品质等肉用性能的测定与比较。结果表明:12周龄F1代公母活重分别达到1236.67、1063.00g,显著高于其父本贵妃鸡体重(公:1025.67g,母:704.67g)。 F1代平均屠宰率为90.22%,全净膛率为61.67%,同时F1代的活重、屠体重、半净膛重、全净膛重、腿肌重和胸肌重等重要经济性状和部分肉品质指标呈现了很好的杂交优势。杂交F1代充分发挥了亲本的优点,因此我们应该充分利用它们各自的优势进行更深层次的研究以满足市场需求。

  2. Reciprocal exchange of minor components of type 1 and F1C fimbriae results in hybrid organelles with changed receptor specificities

    DEFF Research Database (Denmark)

    Klemm, P; Christiansen, Gunna; Kreft, B

    1994-01-01

    recognition proteins present in the organelles as minor structural elements. The organization of the fim and foc gene clusters encoding these fimbriae, as well as the structures of the organelles, are very similar, although the actual sequence homology of the structural elements is not remarkable; notably...... to epithelial cells in the collecting ducts of the human kidney as well as to cells of various cell lines. This report addresses the question of fimbrial promiscuity. Our data indicate that minor fimbrial structural elements can be exchanged between the two fimbrial systems, resulting in hybrid organelles...... with changed receptor specificity. This is the first study on reciprocal exchange of structural components from two different fimbrial systems....

  3. A New Bitter Gourd F1 Hybrid-‘Ruyu No. 45’%苦瓜新品种如玉45号的选育

    Institute of Scientific and Technical Information of China (English)

    吴水金; 张玉灿; 赖正锋; 张少平; 李跃森; 李祖亮; 张伟光

    2013-01-01

    The bitter gourd F1 hybrid‘Ruyu No. 45’is a medium maturing bred by crossing BAL-22-31 as female parent and Shankugua-45 as male parent.The plant grows vigorously.The first female flower sets on around the 17th node.Both of its main and side vines can bear fruit.The com-mercial product is about 6 cm in length,and 3 cm in diameter.The single fruit weight is about 40 g and the matured fruit is about 200 g.Its fruit shape is pointed in two ends with olive shape and melon rind is dark green in color with pointed longitudinal lump.Its succulent is crispy with bitter heavier.But the aftertaste is sweet.The content of total saponin is high.It is highly resistant to Fusarium wilt and pow-dery mildew.It can yield about 63.0 t·hm-2.Its harvesting time can last 6 months or so.It is suitable for cultivation in the south of Fuzhou,Longyan,Zhangzhou,Quanzhou and Xiamen.%如玉45号是以BAL-22-31为母本,以山苦瓜-45为父本配制而成的中熟苦瓜一代杂种。生长势较强,第1雌花节位第17节左右,主侧蔓均可结瓜;商品瓜长6 cm左右,横径3 cm左右,单瓜质量40 g左右,老熟瓜可达200 g;果实两头较尖,橄榄形,瓜皮深绿色,纵棱尖瘤,肉质脆嫩,苦味较重,回味甘甜,总皂甙含量较高,适合煲汤和深加工;田间鉴定对枯萎病和白粉病的抗性较强,一般产量4200 kg·(667 m2)-1左右,采收期可达6个月,适宜福州以南龙岩、漳州、泉州、厦门等地栽培。

  4. A New Tomato F1 Hybrid - ‘Dongnong 716'%番茄新品种东农716的选育

    Institute of Scientific and Technical Information of China (English)

    张贺; 李景富; 康立功; 王傲雪; 姜景彬; 许向阳

    2011-01-01

    东农716是以05HN06为母本,以05HN01为父本配制而成的中晚熟番茄一代杂种,高抗ToMV、叶霉病、枯萎病和黄萎病.单果质量220~240 g,果实红色,圆形,颜色鲜艳,果面光滑美观,硬度大,耐贮运,货架期达20 d(天)以上.大棚栽培每667 m2产量达10 000 kg以上,适合露地和大棚栽培.%'Dongnong 716' is a new mid-later maturing tomato Fi hybrid, developed by crossing 05HN01 as male parent and 05HN06 as female parent. It is high resistant to ToMV, leaf mold, Fusarium wilt and Verticillium wilt. The fruit is large in size. Its average head weight is 220-240 g. Its fruit is red in color, of regular shape with high firmness. The average yield is over 150 t · hm-2 under large plastic shade. It is suitable for cultivation under large plastic shade and in open field.

  5. 辣椒新品种京辣8号的选育%A New Hot Pepper F1 Hybrid-‘Jingla No.8'

    Institute of Scientific and Technical Information of China (English)

    陈斌; 张晓芬; 耿三省

    2012-01-01

    'Jingla No. 8' is a new Fi hybrid developed by crossing inbred lines 04-60 as female parent and 04-136 as male parent. It has mid-early maturity and strong sustainable fruit setting ability. The fruit can preserve good shape throughout the whole growing period. The fruit is of short horn shape with green and smooth skin. The fruit is about 16 cm in length, 5.6 cm in diameter, and 0.4 cm in flesh thickness. The single fruit weight is 90-150 g. It is resistant to TMV and middle resistant to CMV. It can yield about 52.5 t · hm-2. It is suitable for cultivation in protected fields in Yangzi River Basin.%京辣8号是以优良辣椒自交系04-60为母本,以甜椒自交系04-136为父本配制而成的中早熟辣椒一代杂种.中早熟,持续坐果能力强,整个生长季果形保持良好.果实短锥牛角形,以2~3心室为主,青熟果翠绿色,果面光滑.果实纵径约16 cm,横径约5.6 cm,肉厚约0.4 cm,单果质量90~150 g.抗TMV,中抗CMV,对疫病的耐性与对照湘研13号相当.每667 m2产量3 500 kg左右,适于长江流域春秋及秋延后大棚、小拱棚种植.

  6. 樱桃番茄新品种美奇的选育%A New Cherry Tomato F1 Hybrid-‘Meiqi’

    Institute of Scientific and Technical Information of China (English)

    钱健康; 黄浅; 田炜; 刘杰; 李国臣

    2015-01-01

    ‘Meiqi’is a new cherry tomato hybrid developed by crossing‘MT0218’as female parent and‘MT0235-1’as male parent. This variety belongs to limited growth type. The fruit is of high oval shape. The young fruit has no green shoulder,and the matured fruit is red in color. The average single fruit weight is about 17 g. Its fruit hardness is high. It is tolerant to storage and transportation. The fruit tastes sweet&sour and its quality is good. The results of field investigation show that it is resistant to virus disease,leaf mold ,bacterial wilt,etc. Its average yield is about 45 t·hm-2. It is suitable to be planted in greenhouse or open field in Beijing,Liaoning, Henan,Shaanxi,Hunan,Jiangsu,Zhejiang and Hainan Provinces in spring.%美奇是以MT0218为母本,以MT0235-1为父本育成的樱桃番茄一代杂种。有限生长类型,果实高圆形,幼果无果肩,成熟果大红色,平均单果质量17 g左右。果实硬度高,耐贮运,口味甜酸,品质优。田间调查结果表明,美奇的病毒病、叶霉病和青枯病等多种病害发病较轻或未发生。每667 m2产量3000 kg左右。适宜在北京、辽宁、河南、陕西、湖南、江苏、浙江和海南等适宜地区春季保护地或露地种植。

  7. Breeding of New Summer Squash F1 Hybrid Chunhu 1%西葫芦新品种春葫一号的选育

    Institute of Scientific and Technical Information of China (English)

    武青山; 武峻新; 刘彩虹; 温变英

    2012-01-01

    西葫芦新品种春葫一号是以国外引进品种冬玉经多代自交分离选育的自交系Y-12为母本、以国外引进品种碧玉经多代自交分离选育的长条型自交系8X-64为父本,杂交育成的早熟一代杂交种,该品种表现为早熟、植株矮生、株型紧凑、分枝较少、生长势强、持续结瓜能力强、抗性好、瓜条匀称、嫩瓜皮浅绿、有光泽、商品性好等特点,一般每公顷产73 500 kg左右.适宜于早春保护地和露地等种植.%Chunhu 1 is a new summer squash F] hybrid crossed by female parent Y-12 and male parent 8X-64. The self-line Y-12 was bred from Dongyu which was a foreign summer squash variety, and the self-line 8X-64 was selected from foreign and long shape summer squash Biyu. The characteristics of the variety are low and compact plant shape, early maturity, small branch, vigorous growth, strong resistance and good fruit quality. Its ability of sustainable producing fruits is very strong and the fruit shape is symmetrical. The fruit skin is light green and smooth. The total yield is about 73 500 kg/hm2. It could be well cultivated in open and early-spring protected fields.

  8. 昼夜开花萱草杂交后代花朵开闭时间%Opening and Clo sing Time of F1 Hybrids of Daylily (Hemerocallis fulva) and Nightlily (Hemerocallis citrina)

    Institute of Scientific and Technical Information of China (English)

    贾贺燕; 高亦珂; 何琦; 朱琳; 张启翔

    2014-01-01

    萱草属( Hemerocallis)植物依据花朵的开放时间分为夜间开花型和白天开花型两类。为研究萱草花朵开放和闭合的规律,对3种夜间开花的萱草种和品种以及4种白天开花萱草品种进行了杂交,设计了4对杂交组合,每个组合包括正反交,共获得F1代386株。此外,研究了16个辅助杂交组合,后代共208株。2012年和2013年夏季对全部植株的花朵开放和闭合时间进行观测。对于花朵开放时间,北黄花菜( Hemerocallis lilio-as-phodelus )和黄花菜( H.citrina)做亲本的杂交组合后代中99.74%的植株表现为夜间开放;黄花菜品种‘四月花’(H.‘April Flower’)做亲本的各杂交组合中夜间开花的‘诺米路’ב四月花’杂交组合的后代全部表现为夜间开放;与白天开花型杂交的3个组合后代中夜间开放类型与白天开放类型株数的比例接近1∶1或3∶1。花朵闭合时间变异较大,黄花菜和北黄花菜做亲本的杂交后代主要集中在夜间和白天闭合;‘四月花’杂交后代在一天中各个时段均有闭合。结果表明:萱草花朵开放与闭合是受不同基因控制的;花朵开放受一个主基因控制,夜间开花性状是显性,白天开花则为隐性性状;控制花朵开放时间的基因属于核基因;萱草花朵闭合时间表现复杂,可能受多基因调控。%There are two types of Hemerocallis, day blooming species and night blooming species .We studied the genetic devel-opment of the flower opening and closing time in Hemerocallis.We designed the crossing experiments including 4 combina-tions and 16 auxiliary combinations between nocturnal blooming daylily species and diurnal blooming cultivars .We ob-served the flowering behavior of all 594 F1 hybrids in 2012 and 2013.For flower opening time , 99.74%of the F1 hybrids of H.citrina and H.lilio-asphodelus were highly consistent and all of them showed

  9. 脱水加工型洋葱新品种白峰的选育%A New Onion F1 Hybrid for Dehydration — ‘Baifeng’

    Institute of Scientific and Technical Information of China (English)

    曾爱松; 严继勇; 尹钟守; 宋立晓; 高兵

    2011-01-01

    白峰为白皮脱水加工型洋葱一代杂种.不育系70A和保持系70B均来源于荷兰材料Wll的分离后代,父本来源于美国资源W0l自交后代99-3-9-16B.白峰为中熟品种,播种至收获约250d(天);株高65~70 cm,管状功能叶8~9片;鳞茎近圆球形,外皮白色,有光泽;球横径7.5~8.5 cm,纵径6.5~8.5 cm,单球质量230~250g,内部鳞片乳白色,辣味轻,甜度适中,肉质紧实,干物质和VC含量高,抽薹率、分球率低;每667 m2产量5 000 kg左右;田间对紫斑病、霜霉病、灰霉病的抗性与Nube、白岩相当,适宜我国中长日照大部分地区露地栽培.%Dehydrated onion ' Baifeng' is a new Fi hybrid developed by crossing male sterile line with a male parent. Both the female parent ( also called male sterile line 70A ) and its maintainer line 70B originated from a Netherland variety Wl 1. The male parent came from the inbred line 99-3-9-16B, which is the self-pollinated progeny of American variety W01. ' Baifeng' has middle maturity. The period from sowing to bulb harvesting is about 250 days. Its plant height is 65-70 cm, with 8-9 functional leaves. It has round white bulb with 7.5-8.5 cm transverse diameter and 6.5-8.5 cm vertical diameter. The average weight of single bulb is 230-250 g. The white consolidated interior smells mild with sweet pungency. It has higher dry matter and VC contents. Its yield is about 75 t · hm-2. It is resistant to purple stain, downy mildew and grey mould similarly to ' Nube' and ' Baiyan'. It is suitable for cultivation in open fields in most part of China with middle and long sunlight.

  10. Main: O2F1BE2S1 [PLACE

    Lifescience Database Archive (English)

    Full Text Available quences of be2S1 promoter; F1 is hybrid C/G box; O2; opaque-2; be2S1; F1; seed; Brazil nut tree (Bertholletia excelsa); TCCACGTCGA ... ...O2F1BE2S1 S000162 17-May-1998 (last modified) kehi opaque-2 recognition site F1 in Bertholletia excelsa (Bra...zil nut tree) 2S storage protein gene (be2S1); O2 protein binds to F1, F2 and F3 se

  11. IZK OLIMP F1 - NEW BULGARIAN TOMATO VARIETY FOR PROCESSING

    Directory of Open Access Journals (Sweden)

    Daniela Ganeva

    2015-12-01

    Full Text Available The hybrid IZK Olimp F1 is developed by a team at the Maritsa Vegetable Crops Research Institute, Plovdiv as a result of hybridization between female line М-441 and male line R-469. The F1 hybrid was tested in the Executive Agency for Variety Testing, Field Inspection and Seed Control in 2009-2010. It was recognised as a new tomato F1 hybrid variety by the Expert commission in 2009 and has a certificate №10987/ 31.08.2012 issued by the Patent Office of Republic of Bulgaria. Hybrid IZK Olimp F1 is a determinate, high-yielding tomato variety for mid-early field production. The total yield and earliness of this F1 hybrid are close to those of the hybrid var. Vodolei F1 and exceeds the direct var. Bela and var. Zhaklin. The fruits are oval-elongated, with an average weight of 55-68 g, uniform red coloured, thick, firm, crack resistant, with small and low pedicle hole. Being with good chemical and technological properties this hybrid is suitable for processing.

  12. New insights into ocean sunfish (Mola mola) abundance and seasonal distribution in the northeast Atlantic

    NARCIS (Netherlands)

    Breen, Patricia; Cañadas, Ana; Ó Cadhla, Oliver; Mackey, Mick; Scheidat, Meike; Geelhoed, Steve C.V.; Rogan, Emer; Jessopp, Mark

    2017-01-01

    The ocean sunfish, Mola mola, is the largest teleost fish in the world. Despite being found in all oceans of the world, little is known about its abundance and factors driving its distribution. In this study we provide the first abundance estimates for sunfish in offshore waters in the northeast

  13. Energy and Protein Requirements for Maintenance of Doper Sheep and Hu Sheep Hybrid F1 Ewes during Pregnancy%杜湖杂交 F1代母羊妊娠期能量和蛋白质维持需要量

    Institute of Scientific and Technical Information of China (English)

    马铁伟; 聂海涛; 张浩; 纪宇; 王震; 邓凯平; 王锋

    2016-01-01

    The aim of this study was to determine the energy and protein requirements for maintenance of Dor-per and Hu hybrid F1 ewes during pregnancy and to provide data for formulating diet for the mutton sheep. The experiment consisted of feeding experiment, digestion metabolism experiment and respirometary experiment. Fifteen sheep with body weight of (43.34±1.37) kg at the age of nine months were used. After the treatments of synchronization of estrus and free mating, the sheep were assigned randomly into three groups with five e-wes in each group, which were ad libitum ( Ad) group, 70% Ad group and 50% Ad group. Feces and urine were collected using total feces collection method on 40, 100 and 130 days of pregnancy;meanwhile, respiro-metary experiment was conducted, and metabolic parameters of nitrogen and energy at different periods of pregnancy were calculated;finally the requirements of net nitrogen, net energy and metabolic energy for main-tenance of the cross combination during pregnancy were calculated by establishing the allometric regressions re-spectively between nitrogen intake and nitrogen retention, the logarithm of heat production and metabolizable energy intake according to the results of digestion metabolism experiment and respirometary experiment. The re-sults showed as follows:1) the apparent digestibility of nitrogen on 40, 100 and 130 days of pregnancy were 56.16% to 62.24%, 60.43% to 65.47% and 58.79% to 62.01%, respectively. 2) The apparent digestibility of gross energy on 40, 100 and 130 days of pregnancy were 51. 18% to 64. 25%, 60. 86% to 66. 17%, and 63.20%~67.36%, respectively. 3) Requirements of net nitrogen, net energy and metabolic energy for mainte-nance of this breed on 40, 100 and 130 days of pregnancy were 287.9, 301.3 and 430.7 mg/(kg W0.75·d), 194.01, 244.59 and 256.08 kJ/(kg W0.75·d), and 283.18, 412.26 and 468.56 kJ/(kg W0.75·d), respec-tively. The research defines the energy and protein requirement parameters for Dorper sheep

  14. 通过种间杂交选育加工黄瓜新品种宁佳1号%A New Pickling Cucumber F1 Hybrid Bred from Interspecific Hybridization

    Institute of Scientific and Technical Information of China (English)

    陈龙正; 陈劲枫; Jack Staub; 钱春桃

    2005-01-01

    以黄瓜属(Cucumis)野生种(C.hystrix Chakr.)为母本,以栽培黄瓜(C.sativus L.)北京截头为父本,通过远缘杂交导入外源种质,结合多代回交、自交获得黄瓜新种质7012A.将7012A与美国威斯康辛大学选育的雌性系黄瓜(7011A)杂交获得F1,与亲本及美国推广加工黄瓜品种进行品种比较试验,结果表明:F1具有超亲优势,结瓜整齐,营养价值高,加工品质良好.植株长势强,主侧蔓均有结瓜能力,主蔓结瓜为主,后期产量高.

  15. Comparison of tetraploid blastocyst microinjection of outbred Crl:CD1(ICR), hybrid B6D2F1/Tac, and inbred C57BL/6NTac embryos for generation of mice derived from embryonic stem cells.

    Science.gov (United States)

    Kirchain, Sharron M; Hayward, Alison M; Mkandawire, John M; Qi, Peimin; Burds, Aurora A

    2008-04-01

    Embryo electrofusion and tetraploid blastocyst microinjection is a modification of the traditional embryonic stem cell (ES cell)-based method to generate targeted mutant mice. Viability of tetraploid embryos is reportedly lower than with diploid embryos, with considerable interstrain variation. Here we assessed fetus and pup viability after ES cell microinjection of tetraploid blastocysts derived from outbred, hybrid, and inbred mice. Two-cell mouse embryos (C57BL/6NTac [B6], n = 788; B6D2F1/Tac [BDF1], n = 1871; Crl:CD1(ICR) [CD1], n = 1308) were electrofused; most resultant tetraploid blastocysts were injected with ES cells and surgically transferred into pseudopregnant recipient mice. Reproductive tracts were examined at midgestation for embryologic studies using B6 and BDF1 blastocysts; implantation sites and viable fetuses were counted. Pregnancies were carried to term for studies of targeted mutant mice using BDF1 and CD1 blastocysts, and pup yield was evaluated. Electrofusion rates of 2-cell embryos did not differ among B6, BDF1, and CD1 mice (overall mean, 92.8% +/- 5.4%). For embryologic studies, 244 B6 blastocysts were surgically transferred and 1 fetus was viable (0.41%), compared with 644 BDF1 blastocysts surgically transferred and 88 viable fetuses (13.7%). For targeted mutant mouse studies, 259 BDF1 blastocysts were surgically transferred yielding 10 pups (3.9%); 569 CD1 blastocysts yielded 44 pups (7.7%).

  16. 秋季专用平头甘蓝新品种锦秋60的选育%Breeding of Jinqiu 60, a New Cabbage F1 Hybrid with Flattened-shaped Head in Autumn

    Institute of Scientific and Technical Information of China (English)

    曾爱松; 宋立晓; 高兵; 严继勇

    2015-01-01

    Jinqiu 60 is a middle-maturing autumn cabbage F1 hybrid with flattened-shaped head, which was developed by Vegetable Crops Institute of Jiangsu Academy of Agricultural Sciences. It takes 70 days from transplanting to harvest, and Jinqiu 60 is suitable to be planted in South China in autumn. It has good adaptability and strong resistance to black rot, turnip mosaic virus (TuMV) and cabbage Fusarium wilt (CFW). Its leaf head is green and compact and has good quality. Its average head weight is about 1.5 kg and its yield is about 3 650 kg/667 m2.%锦秋60是江苏省农业科学院蔬菜研究所结合小孢子培养技术与常规育种技术选育的秋季专用平头中熟甘蓝新品种。该品种适宜我国南方地区秋季栽培,中熟,定植后70 d左右收获,适应性好,抗黑腐病、病毒病、枯萎病等病害;球形美观,颜色绿,品质佳;单球质量1.5 kg左右,每667 m2产量3650 kg左右。

  17. Influence on longevity of blueberry, cinnamon, green and black tea, pomegranate, sesame, curcumin, morin, pycnogenol, quercetin, and taxifolin fed iso-calorically to long-lived, F1 hybrid mice.

    Science.gov (United States)

    Spindler, Stephen R; Mote, Patricia L; Flegal, James M; Teter, Bruce

    2013-04-01

    Phytonutrients reportedly extend the life span of Caenorhabditis elegans, Drosophila, and mice. We tested extracts of blueberry, pomegranate, green and black tea, cinnamon, sesame, and French maritime pine bark (Pycnogenol and taxifolin), as well as curcumin, morin, and quercetin for their effects on the life span of mice. While many of these phytonutrients reportedly extend the life span of model organisms, we found no significant effect on the life span of male F1 hybrid mice, even though the dosages used reportedly produce defined therapeutic end points in mice. The compounds were fed beginning at 12 months of age. The control and treatment groups were iso-caloric with respect to one another. A 40% calorically restricted and other groups not reported here did experience life span extension. Body weights were un-changed relative to controls for all but two supplemented groups, indicating most supplements did not change energy absorption or utilization. Tea extracts with morin decreased weight, whereas quercetin, taxifolin, and Pycnogenol together increased weight. These changes may be due to altered locomotion or fatty acid biosynthesis. Published reports of murine life span extension using curcumin or tea components may have resulted from induced caloric restriction. Together, our results do not support the idea that isolated phytonutrient anti-oxidants and anti-inflammatories are potential longevity therapeutics, even though consumption of whole fruits and vegetables is associated with enhanced health span and life span.

  18. Ocean sunfish rewarm at the surface after deep excursions to forage for siphonophores.

    Science.gov (United States)

    Nakamura, Itsumi; Goto, Yusuke; Sato, Katsufumi

    2015-05-01

    Ocean sunfish (Mola mola) were believed to be inactive jellyfish feeders because they are often observed lying motionless at the sea surface. Recent tracking studies revealed that they are actually deep divers, but there has been no evidence of foraging in deep water. Furthermore, the surfacing behaviour of ocean sunfish was thought to be related to behavioural thermoregulation, but there was no record of sunfish body temperature. Evidence of ocean sunfish feeding in deep water was obtained using a combination of an animal-borne accelerometer and camera with a light source. Siphonophores were the most abundant prey items captured by ocean sunfish and were typically located at a depth of 50-200 m where the water temperature was Ocean sunfish were diurnally active, made frequently deep excursions and foraged mainly at 100-200 m depths during the day. Ocean sunfish body temperatures were measured under natural conditions. The body temperatures decreased during deep excursions and recovered during subsequent surfacing periods. Heat-budget models indicated that the whole-body heat-transfer coefficient between sunfish and the surrounding water during warming was 3-7 times greater than that during cooling. These results suggest that the main function of surfacing is the recovery of body temperature, and the fish might be able to increase heat gain from the warm surface water by physiological regulation. The thermal environment of ocean sunfish foraging depths was lower than their thermal preference (c. 16-17 °C). The behavioural and physiological thermoregulation enables the fish to increase foraging time in deep, cold water. Feeding rate during deep excursions was not related to duration or depth of the deep excursions. Cycles of deep foraging and surface warming were explained by a foraging strategy, to maximize foraging time with maintaining body temperature by vertical temperature environment. © 2015 The Authors. Journal of Animal Ecology © 2015 British

  19. EST-SSR Markers Development from Cucurbita and Their Use in Purity Testing of F1 Hybrid Seed%南瓜属EST-SSR标记的开发及在杂种纯度鉴定中的应用

    Institute of Scientific and Technical Information of China (English)

    张国裕; 张帆; 姜立纲; 翟伟卜; 李海真

    2011-01-01

    Expressed Sequence Tags(ESTs) are a source of simple sequence repeats (SSRs) that can be used to develop molecular markers for genetic studies. The object of the present study is to develop EST-SSR markers from Cucurbita and uses these markers in quickly purity testing of F1 hybrid seed. The availability of 1457 ESTs for cucurbita,which documented in GenBank, provided a unique opportunity to develop EST-SSR markers, and 215 SSRs were identified among these non-redundant EST sequences. These SSRs contained 2 - 6 bp nucleotide motifs including 111 different motif types. The trinucleotide repeat is the dominant type,accounting for 40% with 86 motifs, and the frequency of occurrence of dinucleotide repeat is 28. 84% with 62 motifs. Among these motifs,CT motif (21 ,9. 77% ) was the most common type,and then were TC and AAG motifs,accounting for 6. 05% and 5. 58% , respectively. A total of 215 primer pairs were designed and 43 of them were synthesized and used to determine their usability by amplification in 4 Cucurbita inbred lines. The results show that 28 of them could successfully amplify, accounting for 65. 12% of testing primer pairs. Then these primer pairs were used to test the F, hybrid seed purity in Cucurbita. Seed genetic purity from 13 combinations ranged from 79. 2% to 100. 0% .which were in high accordance with those from field grow-out trials. Taken together, this study reported an effective and feasible approach to develop SSR markers for cucurbita,and demonstrated their usefulness in purity testing of hybrid squash seed.%为利用SSR标记进行快速、准确的南瓜杂交种纯度分析,进行了南瓜EST-SSR标记的开发.从NCBI数据库下载1 457条南瓜属作物EST序列,去除冗余序列后进行SSR位点搜索,共得到215条含有SSR位点的EST序列.这些SSR位点包含111种重复基元,其中二核苷酸(62个,占28.84%)和三核苷酸(86个,占40.0%)重复类型占主导地位;重复基元中出现最多的是CT(21个,占9.77

  20. A New Hot Pepper F1 Hybrid Specially for Drying Use——'Hangjiao No. 10'%制干专用型辣椒新品种航椒10号的选育

    Institute of Scientific and Technical Information of China (English)

    霍建泰; 罗爱玉; 高彦辉; 危金彬; 李想荣; 胡小明; 张建东; 蒲永鸿

    2011-01-01

    ‘Hangjiao No.10’ is a new hot pepper F1 hybrid specially for dry use. It is developed by crossing inbred lines 022-5-1 and 023-3-2. It has vigorous growth and middle maturity. It has about 34 fruits per plant. The fruit is of long tapered shape. The dried peper is purplish red in color with strong pungent flavor. It is 13.6 cm in length, 1.6 cm in diameter, 2.6 g in weight. This cultivar is resistant to Phytophthora capsici Leonian and virus disease. The average dried-pepper yield is 5.25 t · hm-2. It adapts to open field cultivation in northwest and North China planting.%航椒10号是以经航天诱变处理选育的自交系022-5-1为母本,以023-3-2为父本配制而成的制干专用型辣椒一代杂种.植株生长势强,中熟,单株结果34个左右.果实长指形,干椒紫红色,有光泽,辣昧极强,纵径13.6 cm,横径1.6 cm,单果质量2.6 g.田间凋查表明:抗辣椒疫病、病毒病,每667 m2产干椒350 kg左右.适宜西北、华北地区露地种植.

  1. 食用兼观赏型南瓜新品种宝仁南瓜的选育%Breeding of Baoren Pumpkin, a New Edible and Ornamental Pumpkin F1 Hybrid

    Institute of Scientific and Technical Information of China (English)

    张焕春; 尹国香; 赵明; 夏秀波; 曹守军; 刘学卿

    2013-01-01

      Baoren pumpkin was a new pumpkin F1 hybrid, which was bred by taking Y-8-8-1-6-4 as female parent and Y-20-5-6 as male parent. It grew vigorously with excellent fruit-setting and continuous fruit-setting capacity, and its first female flower usually set on 8th-10th node. The fruit was round and flat with salmon pink fruit peel, and the average single fruit weight was about 0.8-1.2 kg. It was resistant to powdery mildew and virus disease, and its seed kernel was naked. It tasted well and had good commercial traits. Its yield was 3 170.4 kg/667 m2, which was 15.6% higher than that of the control cultivar Hongmi pumpkin.%  宝仁南瓜是Y-8-8-1-6-4和Y-20-5-6配制而成的一代杂交种,其生长势强,第1雌花节位8~10节,坐果性好,连续坐果能力强;果实扁圆形,果皮橙红色,单瓜质量0.8~1.2 kg,可观赏性强;种子裸仁;田间表现较抗白粉病和病毒病,商品性好,风味品质佳;平均每667 m2产量为3170.4 kg,比对照红蜜南瓜增产15.6%。

  2. 耐贮运番茄新品种红石串的选育%Breeding of Hongshichuan, a New Tomato F1 Hybrid with Excellent Tolerance to Storage and Transportation

    Institute of Scientific and Technical Information of China (English)

    周火强; 梁成亮

    2012-01-01

      红石串是以04-1-6-3-5-7-9 为母本、F12-2-16-2 为父本配制的中熟番茄一代杂种。无限生长型,果实深红色,扁圆形,果实大小均匀,平均单果质量150~200 g,耐贮运,可溶性固形物含量5.60%,总糖含量3.84%,VC 含量 31.03 mg/100 g(FW),有机酸含量0.40%,每667 m2产量4 000 kg 左右。已在华中、华南、西南各省大面积推广种植。%  Hongshichuan is a new medium-maturing tomato F1 hybrid by taking 04-1-6-3-5-7-9 and Fl2-2-16-2 as female and male parent respectively. It is an infinite growth type, and it has large bell-shaped fruits, with the average single fruit weight of 150-200 g. The fruit is deep red in color and suitable for transportation and storage, with soluble solids content of 5.6%, total sugar content of 3.84%, vitamin C content of 31.03 mg/100 g (FW) and organic acid content of 0.40%, and its yield is 4 000 kg/667 m2. It has be extended widely in central, southern and south-western China.

  3. A New Cucumber F1 Hybrid-‘Xianghuanggua No.4’%夏秋黄瓜新品种湘黄瓜4号的选育

    Institute of Scientific and Technical Information of China (English)

    王安乐; 朱海泉; 唐术江

    2001-01-01

    湘黄瓜4号系从200余份黄瓜品种资源中筛选出的优良自交系L88-9和T5-8杂交配制而成的一代杂种。植株生长势强,以主蔓结瓜为主,果实长棒形,长约31cm,瓜把长约3.6cm,平均单果质量297g,果皮深绿色、刺瘤较多,突起较明显,瓜把无苦味,肉质脆,风味佳,商品性好,从播种至采收45~50d(天),平均每667m2产量2405kg,比津研7号增产39%,田间表现抗病毒病、霜霉病和枯萎病。%Xianghuanggua No.4 is a new F1 hybrid by erossing line L88-9 with T5-8. It grows vigorously with high resistant to downy mildew,virus and Fusarium wilt. It takes only 45-50 days from sowing to first harvesting. It yields 36 t*hm-2, 39% higher than that of ‘Jinyan No.7’. Average fruit weight is about 297g. Fruits are dark green,sweet and dcrispy with high marketable percentage. The fruit neck is about 3.6cm with is about 1/8 of fruit length.

  4. 乌珠穆沁羊(♀)×萨福克羊(♂)杂交F1产肉性能分析%The Performance of Meat Production in Hybrid F1 Sheep of Ujumuqin sheep(♀)×Suffolk Sheep(♂)

    Institute of Scientific and Technical Information of China (English)

    刘锋

    2010-01-01

    @@ 近年来,兴安盟引进萨福克羊与乌珠穆沁羊杂交,杂交F1羊增重效果显著.据2002年与2003年测定,放牧条件下,108只杂交F1羊6月龄平均活重36.25kg,而31只乌珠穆沁羊6月龄平均活重27.60kg,平均增重8.65kg,差异极显著.

  5. Karyotype of the Hybrid F1 of Snapper(Lutjanus erythropterus ♀×L.sebae ♂)and Their Parents%红鳍笛鲷(♀)与千年笛鲷(♂)杂交F1代及其亲本的核型研究

    Institute of Scientific and Technical Information of China (English)

    尹绍武; 廖经球; 邓勤; 陈国华; 刘万川

    2008-01-01

    采用胸腔活体注射PHA及秋水仙素溶液,取头肾细胞,低渗处理,空气干燥法制片,对染色体进行Giemsa染色,研究红鳍笛鲷(♀)与千年笛鲷(♂)杂交F1代及其亲本的核型.结果表明,杂交F1代及其亲本(红鳍笛鲷和千年笛鲷)的核型相同,均为2 n=48 t,NF=48,符合遗传理论.

  6. 杂色鲍与盘鲍杂交F1的早期生物学表现%Early-stage performance of hybrid between Haliotis diversicolor and H. discus discus

    Institute of Scientific and Technical Information of China (English)

    蔡明夷; 柯才焕; 郭峰; 王桂忠; 王志勇; 王艺磊

    2011-01-01

    比较研究杂色鲍(Haliotis diversicolor)(S)与盘鲍(H.discus discus)(J)正反交F1及其亲本自繁后代的卵裂速度和孵化速度,以及杂色鲍与盘鲍杂交F及杂色鲍自繁幼体的成活率.在22~23℃下,杂色鲍自繁后代的早期发育速度快于盘鲍自繁后代.杂色鲍和盘鲍2细胞期分别为授精后45.3 min±8.9 min和59.3min±8.9min.卵裂间隔时间分别为21.7min±2.9min和27.0min±2.6min,孵化时间分别为授精后563.4 min和711.8 min.SJ和JS杂交F的第一次卵裂速度均比他们的双亲慢,2细胞期分别为69.3min±17.5 min和71.7 min±5.8 min;而卵裂间隔时间(τ)表现出较强的母本效应,略长于母本,分别为22.2 min±4.7 min和31.3 min±6.1 min;孵化速度均慢于其母本自繁后代,分别为授精后632.7 min和授精后874.3 min.计算结果表明,自繁和杂交胚胎过程发育经历细胞周期数相当,SS、SJ、JJ和JS的从2细胞到孵化经历时间分别相当于23.9τ、25.4τ、24.1τ和24.8τ.另一方面,SJ杂交F在附着变态阶段大量畸形、死亡,变态后成活率仅为母本自繁组合的1.2%,显现出较高程度的杂交不亲和.本文的研究结果为杂色鲍与盘鲍杂交F的后续应用开发提供了依据,也为进一步研究杂交F的遗传本质及父母本的基因互作关系奠定基础.%In present study, mitotic intervals, hatching speed and survival rate of embryos produced from reciprocal hybrid cross and pure cross of small abalone (S, Haliotis diversicolor) and Japanese abalone (J, H. discus discus) were investigated. At 22~23 ℃, the small abalone developed faster than the Japanese abalone at early stage. The times to reach the two-cell stage were 45.3 min ± 8.9 min post insemination (p.i.) for the small abalone and 59.3 min ± 8.9 min for the Japanese abalone. The small abalone took 21.7 min ± 2.9 min to complete a cleavage cycle and 563.4 min to hatch out, while the Japanese abalone took 31.3 min ± 6.1 min and 711.8 min

  7. Study on the Differences of Bollworm-Resistance and Bt Protein Content between Transgenic Bt Cotton F1 Hybrids and Insect-Resistant Parent%抗虫杂交棉F1代与亲本Bt蛋白表达量及抗虫差异性研究

    Institute of Scientific and Technical Information of China (English)

    刘海涛; 郭香墨; 夏敬源

    2000-01-01

    对转Bt基因抗虫杂交棉正、反交F1代与抗虫亲本的Bt蛋白表达量及抗棉铃虫差异性研究表明:抗虫亲本与其杂种F1代均高抗棉铃虫,但抗虫亲本的抗虫性略好于其杂种F1代,并且明显地高于非抗虫亲本;正、反交杂种P1代间的抗虫性几乎没有差异.生长前期的抗虫性好于后期,同一时期嫩叶或侧枝生长点的抗虫性好于幼蕾.抗虫亲本叶片和花瓣的Bt蛋白含量明显地高于其杂种Fl代,抗虫亲本功能叶的Bt蛋白含量明显地高于其上部非功能叶,而杂种F1代功能叶的Bt蛋白含量则明显地低于其上部非功能叶.盛花期后至吐絮期前,叶片和花瓣的Bt蛋白表达量明显增加,在抗虫亲本中表现最为明显.与叶片相比,在花瓣中检测到的Bt蛋白含量极低.正、反交F1代间的Bt蛋白表达量差异较小或无规律可循.

  8. 低温胁迫对砧木南瓜F1代及其亲本幼苗的影响%Effects of Low Temperature Stress on Seedlings of Rootstoch Hybrid F1 (Cucurbita maxima × Cucurbita moschata) and Their Parents

    Institute of Scientific and Technical Information of China (English)

    孙吉庆; 孙令强; 李敏

    2013-01-01

    以印度南瓜(母本,FP)、中国南瓜(父本,MP)及其种间杂交F1(印度南瓜×中国南瓜)代幼苗为试材,采用5℃低温处理幼苗2d,分别测定低温胁迫前后幼苗根系活力、电导率和丙二醛(MDA)含量以及保护酶活性的变化.结果表明:低温胁迫后F1代的根系活力最强,相对电导率最低,MDA含量最低,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性最高,其次是母本印度南瓜,父本中国南瓜最低.这些生理指标的变化与砧木南瓜的耐寒性密切相关,即F1代的耐寒性最强,印度南瓜次之,中国南瓜最弱.

  9. 保护地黄瓜新品种津优308号的选育%A New Cucumber F1 Hybrid-‘Jinyou No.308’

    Institute of Scientific and Technical Information of China (English)

    李愚鹤; 张利东; 黄洪宇; 李加旺

    2014-01-01

    ‘Jinyou No.308’ is a new cucumber F1 hybrid bred by crossing inbred line X8-24 as female parent and 35-1-12-6 as male parent. ‘Jinyou No.308’ has strong growth vigor and leaf of medium size. It is resistant to downy mildew, fusarium wilt, angular leaf spot, and medium resistant to powdery mildew. It shows good adaptability to low temperature and weak light. It is not premature aging and has strong sustainable fruit setting ability. It is also resistant to high temperature at later growth stage and still fruiting under the condition of 34-36 ℃. The fruit is erect and straight, about 34 cm in length with short neck. The single fruit weight is about 220 g. The fruit has glossy dark green rind. There are many spines on fruit surface. The yield is over 240 t·hm-2 in overwinter solar greenhouse and about 105 t·hm-2 in early spring plastic shelter. It is suitable to be cultivated in overwinter solar greenhouse and early spring plastic shelter.%津优308号是以X8-24为母本,以35-1-12-6为父本配制而成的保护地黄瓜一代杂种。植株生长势强,叶片中等大小;抗霜霉病、枯萎病、角斑病,中抗白粉病;耐低温弱光性好,不早衰,持续坐果能力强;生长后期耐高温,在34~36℃条件下亦可正常结瓜。瓜条顺直,瓜把短,皮色深绿,光泽度好,刺密,瘤中等,商品性好;瓜条长34 cm左右,单瓜质量220 g左右,日光温室越冬栽培产量16000 kg·(667 m2)-1以上,早春大棚栽培产量可达7000 kg·(667 m2)-1,适宜我国北方地区越冬温室和早春大棚栽培。

  10. 不结球白菜新品种速俊028的选育%A New Non-heading Chinese Cabbage F1 Hybrid-‘Sujun 028’

    Institute of Scientific and Technical Information of China (English)

    黄志银; 张斌; 闻凤英; 刘晓晖; 罗智敏; 王超楠; 李梅

    2014-01-01

    ‘Sujun 028’is a new non-heading Chinese cabbage F1 hybrid bred by crossing 2 self-incompatible lines 08-411-5s and 07-N2 kangre-3s.It grows fast with beautiful plant type.It is about 20 cm in height and about 25 cm in width.The leaf is glossy green and the petiole is short without wax powder.It is early maturing.In summer and autumn sowing,it takes only 20 days to bean waist.It can be planted with appropriate density.The best planting density is 8 cm in row spacing and 15 cm in line spacing.It is highly resistant to virus disease,downy mildew and resistant to soft rot.It has wide adaptability,and is suitable for summer and autumn cultivation all over the country.For winter and spring cultivation,it can be grown in open fields in the southern areas of the Yangtze River,but in the norther areas of the Yangtze River it has to be planted in protected fields.%速俊028是由两个自交不亲和系08-411-5s和07-N2抗热-3s配制而成的不结球白菜一代杂种。株型美观,株高20cm左右,开展度25cm左右;叶色亮绿,叶柄较短、勺状、碧绿无蜡粉,基部头大、束腰;早熟,生长速度快,夏秋季播种20d(天)后开始束腰;可适当密植,最佳种植密度为株距8cm,行距15cm;高抗病毒病、霜霉病,抗软腐病;适应性广,全国各地夏秋季均可种植,长江以南地区冬春季可露地栽培,长江以北地区秋冬季和冬春季需保护地种植。

  11. 早熟机采加工番茄新品种新番48号的选育%An Early Maturing, Mechanical-harvesting Processing Tomato F1 Hybrid-‘Xinfan No.48’

    Institute of Scientific and Technical Information of China (English)

    曾沂辉; 薛琳; 王诚军; 高明; 金凤媚; 祝晏兵; 李英; 孙秀霞; 李守明

    2014-01-01

    ‘Xinfan No.48’ is an early maturing processing tomato F1 hybrid developed by crossing F19-3-1-1 as female parent and TD-06-2 as male parent. It is suitable for mechanical-harvest or manual one-time harvest. It takes 87 days for growing period. The plant is of open type with dark green leaves. The plant is 65 cm in hight with 6-7 branches. The first inflorescence usually sets at the 7th node. Its fruit is of oval shape with shinning deep red color. The fruit shape index is 1.2. Its average single fruit weight is 70-75 g. The lycopenr content is 123 mg·kg-1, soluble solid content is 4.64 %, and total acid is 0.38%. Its fruits can stand heavy pressure and tolerant to storage in field condition. Its average yield can reach over 120 t·hm-2. It is suitable to be planted in Northern and Southern Xinjiang and Gansu Provinces. It is good for producing tomato paste.%新番48号是以F19-3-1-1 为母本,以TD-06-2为父本配制而成的早熟机采加工番茄一代杂种。直播生育期87 d (天),株高65 cm,株型开展,叶色深绿,6~7个分枝,第1花序节位为第7节,果实椭圆形,果形指数1.2,果色深红,光亮,无绿果肩,2~3心室,平均单果质量70~75 g。番茄红素含量123 mg·kg-1,可溶性固形物含量4.64%,总酸含量0.38%。果实耐压,田间耐贮性较好,适合机械化采收或人工一次性采收。平均每667 m2产量8 000 kg以上。适合新疆南北疆、甘肃等地种植,用于生产番茄酱。

  12. A New Summer Squash F1 Hybrid with High Resistance to ZYMV-‘Jinghu CRV4’%抗ZYMV西葫芦新品种京葫CRV4的选育

    Institute of Scientific and Technical Information of China (English)

    李海真; 张国裕; 张帆; 贾长才; 姜立纲

    2014-01-01

    ∶‘Jinghu CRV4’is a new summer squash F1 hybrid bred by crossing inbred line‘08-19-15-29-18-20-58’as female parent with‘06-2-6-38-15-26-18’as male parent. It was developed by combining marker-assisted selection technology with traditional breeding methods. It is an early maturing variety with mid-vigorous plant growth,short nods and continuous fruit setting ability. Its fruit has glossy light green rind. The plant is of mid-tall cylinder shape with straight and equal size fruit. It is tolerant to high temperature and not easy to premature aging. It has high resistance to ZYMV(Zucchini yellow masaic vius)and powdery mildew. It is suitable to be cultivated in protected and open fields both in southern and northern China.%采用常规育种方法与分子标记辅助选择育种技术相结合,同时采用海南加代穿梭育种方法培育出抗小西葫芦黄化花叶病毒病(Zucchini yellow mosaic virus,ZYMV)西葫芦一代杂种京葫CRV4。父本为06-2-6-38-15-26-18,母本为08-19-15-29-18-20-58。该品种早熟,生长势中等,短蔓;瓜码密,连续结瓜能力强;商品瓜翠绿色,光泽度好,中长柱形,顺直均匀;耐热,不易早衰,适应性强,抗ZYMV和白粉病;适合南北方春秋大棚和露地种植。

  13. Functional dorsoventral symmetry in relation to lift-based swimming in the ocean sunfish Mola mola.

    Science.gov (United States)

    Watanabe, Yuuki; Sato, Katsufumi

    2008-01-01

    The largest (up to 2 tons) and a globally distributed teleost--the ocean sunfish Mola mola--is commonly regarded as a planktonic fish because of its unusual shape including absence of caudal fin. This common view was recently questioned because the horizontal movements of the ocean sunfish tracked by acoustic telemetry were independent of ocean currents. However, direct information regarding their locomotor performance under natural conditions is still lacking. By using multi-sensor tags, we show that sunfish indeed swam continuously with frequent vertical movements at speeds of 0.4-0.7 m s(-1), which is similar to the records of other large fishes such as salmons, marlins, and pelagic sharks. The acceleration data revealed that they stroked their dorsal and anal fins synchronously (dominant frequency, 0.3-0.6 Hz) to generate a lift-based thrust, as penguins do using two symmetrical flippers. Morphological studies of sunfish (mass, 2-959 kg) showed that the dorsal and anal fins had similar external (symmetrical shape and identical area) and internal (identical locomotory muscle mass) features; however, the muscle shape differed markedly. We conclude that ocean sunfish have functional dorsoventral symmetry with regards to the non-homologous dorsal and anal fins that act as a pair of vertical hydrofoils. Although sunfish lack a swimbladder, we found that they are neutrally buoyant independent of depth because of their subcutaneous gelatinous tissue that has low density and is incompressible. Efficient lift-based swimming in conjunction with neutral buoyancy enables sunfish to travel long distances both horizontally and vertically.

  14. Effects of littoral habitat complexity and sunfish composition on fish production

    Science.gov (United States)

    Carey, M.P.; Maloney, K.O.; Chipps, S.R.; Wahl, David H.

    2010-01-01

    Habitat complexity is a key driver of food web dynamics because physical structure dictates resource availability to a community. Changes in fish diversity can also alter trophic interactions and energy pathways in food webs. Few studies have examined the direct, indirect, and interactive effects of biodiversity and habitat complexity on fish production. We explored the effects of habitat complexity (simulated vegetation), sunfish diversity (intra- vs. inter-specific sunfish), and their interaction using a mesocosm experiment. Total fish production was examined across two levels of habitat complexity (low: 161 strands m-2 and high: 714 strands m-2) and two sunfish diversity treatments: bluegill only (Lepomis macrochirus) and bluegill, redear sunfish (Lepomis microlophus), and green sunfish (Lepomis cyanellus) combination. We also measured changes in total phosphorus, phytoplankton, periphyton, and invertebrates to explain patterns in fish production. Bluegill and total fish production were unaffected by the sunfish treatments. Habitat complexity had a large influence on food web structure by shifting primary productivity from pelagic to a more littoral pathway in the high habitat treatments. Periphyton was higher with dense vegetation, leading to reductions in total phosphorus, phytoplankton, cladoceran abundance and fish biomass. In tanks with low vegetation, bluegill exhibited increased growth. Habitat complexity can alter energy flow through food webs ultimately influencing higher trophic levels. The lack of an effect of sunfish diversity on fish production does not imply that conserving biodiversity is unimportant; rather, we suggest that understanding the context in which biodiversity is important to food web dynamics is critical to conservation planning. ?? 2010 John Wiley & Sons A/S.

  15. Functional dorsoventral symmetry in relation to lift-based swimming in the ocean sunfish Mola mola.

    Directory of Open Access Journals (Sweden)

    Yuuki Watanabe

    Full Text Available The largest (up to 2 tons and a globally distributed teleost--the ocean sunfish Mola mola--is commonly regarded as a planktonic fish because of its unusual shape including absence of caudal fin. This common view was recently questioned because the horizontal movements of the ocean sunfish tracked by acoustic telemetry were independent of ocean currents. However, direct information regarding their locomotor performance under natural conditions is still lacking. By using multi-sensor tags, we show that sunfish indeed swam continuously with frequent vertical movements at speeds of 0.4-0.7 m s(-1, which is similar to the records of other large fishes such as salmons, marlins, and pelagic sharks. The acceleration data revealed that they stroked their dorsal and anal fins synchronously (dominant frequency, 0.3-0.6 Hz to generate a lift-based thrust, as penguins do using two symmetrical flippers. Morphological studies of sunfish (mass, 2-959 kg showed that the dorsal and anal fins had similar external (symmetrical shape and identical area and internal (identical locomotory muscle mass features; however, the muscle shape differed markedly. We conclude that ocean sunfish have functional dorsoventral symmetry with regards to the non-homologous dorsal and anal fins that act as a pair of vertical hydrofoils. Although sunfish lack a swimbladder, we found that they are neutrally buoyant independent of depth because of their subcutaneous gelatinous tissue that has low density and is incompressible. Efficient lift-based swimming in conjunction with neutral buoyancy enables sunfish to travel long distances both horizontally and vertically.

  16. Acquisition and Utilization Characteristics of Phosphorus in Hybrid F1 and Parents with Different P Efficiencies under Phosphorus Deficient Condition%缺磷胁迫下不同磷效率小麦品种及其杂种F1的磷吸收利用特性

    Institute of Scientific and Technical Information of China (English)

    李宾兴; 郭程瑾; 王斌; 肖凯; 李雁鸣

    2006-01-01

    在缺磷胁迫下,对磷低效、磷吸收高效和利用高效3个小麦品种及其杂种F1的磷吸收、利用特性进行了研究.结果表明,与磷低效品种Nc37相比,磷吸收高效品种81(85)单株次生根数较多、次生根直径较大、根系干物质重量较大、TTC(氯化三苯基四氮唑)还原力较高和单株全磷量较多.其对磷素吸收量的增加,是根系形态和根体构型改变和对土壤中难溶性磷素利用能力增强两方面共同作用的结果.利用高效品种(蚂蚱麦)具有较高的旗叶酸性磷酸化酶活性和较高的磷利用效率,对于改善植株体内磷的代谢周转和再利用能力,进而提高植株的磷利用效率可能具有较重要作用.在F1-1[Nc37×81(85)],F1-2(Nc37×蚂蚱麦)和F1-3[81(85)×蚂蚱麦]3个杂种F1中,单株次生根数、次生根粗度、单位土体根系干重、根系TYC还原力、植株成熟期全磷量和旗叶酸性磷酸化酶活性的离中优势(Hm)和超高亲优势(Hh)多为正向优势.其单株籽粒产量的Hm和Hh均为正值,分别变化在31.7%~32.8%(Hm)和18.5%~29.6%(Hh)之间.用高效吸收、利用2个不同类型的小麦品种作亲本配制杂交组合,充分利用F1代在磷吸收利用上的杂种优势,对于改善在磷胁迫下小麦的磷素营养可能具有重要作用.

  17. Studies on heterosis of vernal hybrid F1 of male sterile lines of Ven & K Typeal wheat and their relationship with chlorophyll content and zymogram of isoenzyme%Ven、K型小麦雄性不育系春性F1杂种的优势表现及与叶绿素含量和同工酶谱的关系

    Institute of Scientific and Technical Information of China (English)

    魏亦勤; 张改生; 李树华; 李国庆; 刘旺清; 李红霞; 张双喜; 龚月娟; 裘敏; 王平

    2001-01-01

    利用Ven、K型小麦雄性不育系与北方春性普通小麦杂交,研究了Ven、K型春性F1杂种的优势表现及其与叶绿素含量、同工酶谱的关系.结果表明:Ven型和K型杂种F1存在显著的个体杂种优势,在主要农艺性状中尤其以单株穗数、千粒重、单株生产力的杂种优势明显,其中单株穗数对单株生产力的贡献最大;相关分析表明:单株生产力与单株穗数呈正相关,相关系数为0.7122;与每穗粒数和千粒重的相关系数分别为-0.1183和0.4941.灌浆期旗叶、倒二叶叶绿素含量与千粒重的相关性最大,呈正相关.杂种F1优势强的组合其种子和苗期的过氧化物同工酶和酯酶的酶谱一般呈双亲互补型或“杂种型”酶带,该特征可作为杂种优势预测的指标.

  18. New insights into ocean sunfish (Mola mola) abundance and seasonal distribution in the northeast Atlantic.

    Science.gov (United States)

    Breen, Patricia; Cañadas, Ana; Cadhla, Oliver Ó; Mackey, Mick; Scheidat, Meike; Geelhoed, Steve C V; Rogan, Emer; Jessopp, Mark

    2017-05-17

    The ocean sunfish, Mola mola, is the largest teleost fish in the world. Despite being found in all oceans of the world, little is known about its abundance and factors driving its distribution. In this study we provide the first abundance estimates for sunfish in offshore waters in the northeast Atlantic and the first record of extensive sunfish presence in these waters year-round. Abundance estimates and predictive distributions for sunfish in approximately 300,000 km² of the northeast Atlantic were derived from large scale offshore aerial surveys in 2015-2016 using distance sampling techniques. Generalized additive models of sunfish density were fitted to survey data from 17,360 km of line transect effort resulting in minimum abundance estimates of 12,702 (CI: 9,864-16,357) in the summer (Density = 0.043 ind/km²) and 8,223 individuals (CI: 6,178-10,946) (Density = 0.028 ind/km²) in the winter. Density surface models predicted seasonal shifts in distribution and highlighted the importance of the mixed layer depth, possibly related to thermoregulation following deep foraging dives. The abundance estimate and estimated daily consumption of 2,600 tonnes of jellyfish in the northeast Atlantic highlights the need to re-assess the importance of this species in the pelagic ecosystem, and its role in top-down control of jellyfish blooms.

  19. Strategies for improving quality inspection of silkworm F1 hybrids egg products in Guangxi%广西桑蚕一代杂交种成品卵质量检验改进措施探讨

    Institute of Scientific and Technical Information of China (English)

    罗梅兰; 黄旭华; 安春梅; 胡文娟; 甘丽红

    2012-01-01

    [目的]探索桑蚕一代杂交种成品卵质量检验新方法,提高成品卵质量检验的准确性、高效性及可操作性.[方法]分别采用直接计数蚁蚕法、称量计算蚁蚕法、直接计数良卵法,进行蚕种孵化率和良卵数调查,并比较3种方法的调查效率.[结果]采用称量法计算蚁蚕头数与直接计数蚁蚕头数的结果无显著性差异,采用直接计数良卵数与实际良卵孵化值误差达到极显著水平;在效率方面,直接计数良卵法耗时是直接计数蚁蚕法的56.75%,而称量计算蚁蚕法耗时仅是直接计数蚁蚕法的21.83%,节省约4/5的调查时间,工作效率极大提高.[结论]将称量计算蚁蚕法应用于良卵数和孵化率调查是可行的,且具有操作性好、省时、省工、效率高,准确性高等优点.%[Objective]The present study was conduced to develop a new, accurate and efficient method for inspecting quality of silkworm F1 hybrids egg products. [ Method ] Hatching rate and number of good eggs were determined by direct counting of newly-hatched larva, weighing newly-hatched larva and direct counting of good eggs. These three methods were compared with each other for their efficiency. [Result]There was no significant difference between weighing and direct counting on newly-hatched larva, while significant difference occurred between the number of good eggs using direct counting method and the actual number of newly-hatched larva. For efficiency of the method, the time-consuming for direct counting of good eggs was 56.75% of direct counting of newly-hatched larva, while that of weighing method was only 21.83% of direct counting of newly-hatched larva, indicating that approximately 4/5 investigation time was saved so to enhance the work efficiency enormously. [Conclusion]Weighing newly-hatched larva method was found time and labor-saving, highly effective and accurate, and easy to be operated, hence it is feasible to apply weighing method to

  20. The Meat Production Performance of the F1 Generation Hybrid Sheep of German Mutton Merino Sheep and Local Sheep%德国肉用美利奴羊×本地绵羊杂种羊产肉性能的研究

    Institute of Scientific and Technical Information of China (English)

    早热古丽·热合曼; 阿娜古丽·艾山; 阿力木江·斯拉木; 美丽坎木·依明; 阿瓦古丽·依明

    2015-01-01

    Abatract:In this study,the meat production performance of the F1 generation hybrid sheep of German Mutton Merino sheep and local sheep was evaluated. The result showed that the hybrid sheep have the heterosis in terms of adaptability to the local confinedness condition, growth and development and meat production performance in 2013 and 2014. The average weight of F 1 generation of the hybrid sheep in 6 months old was 44.24 kg and was significantly higher than that of the control group (P<0.01), and lean meat percentage was higher and mutton quality was better in 2014. The carcass weight of F1 generation in 6 months old was 6.28 kg higher than that of control group, and the meat percentage and dressing percentage of the F1 generation was 5.11%and 6.92%higher than those of the control group respectively (P<0.05).%对德国肉用美利奴羊(父本)与本地绵羊二元杂交后代羔羊的生长发育、产肉性能以及多胎基因等进行了研究。结果表明,2013年和2014年德×本F1杂交羔羊在当地的舍饲适应性、生长发育和产肉方面均表现出杂种优势,尤其是2014年6月龄德×本F1平均体重达到44.24 kg,比对照组高7.02 kg,差异极显著(P<0.01),且其瘦肉率高、羊肉品质好;经过短期育肥的6月龄杂种羔羊胴体重比对照组提高了6.28 kg,净肉率、屠宰率分别比对照组提高了5.11%和6.92%,差异显著(P<0.05)。

  1. Comparative Analysis on Growth and Morphology in Yangtze River ♀, Zhujiang River ♂ and Their F1 Hybrids of Juvenile Grass Carp%长江草鱼♀×珠江草鱼♂杂交子一代与其亲本一龄阶段生长性能和体型分析

    Institute of Scientific and Technical Information of China (English)

    傅建军; 王荣泉; 刘峰; 宣云峰; 朱树人; 李家乐

    2009-01-01

    [目的]研究杂交草鱼一龄阶段的生长和体型特点.[方法]建立长江自交 YR(长江群体♀×长江群体♂)、珠江自交 ZR(珠江群体♀×珠江群体♂)、杂交 F1(长江群体♀×珠江群体♂)3个组合.为了避免前期生长差异对后期试验的影响,亲鱼催产至鱼苗下塘均同步进行,控制夏花培育过程养殖密度、养殖环境等条件保持一致;另一方面,到50日龄即对3个组合进行剪鳍标记同箱混养,克服不同网箱的影响.对3个组合鱼的生长和形态数据进行测量分析.体重(W)用电子天平测量,全长(TL)、体长(SL)、头长(HL)、体高(BH)和体宽(BW)用游标卡尺测量.绝对增重率 AGR(g/d)=(W2-W1) / ( t2-t1);超亲杂种优势HB(%)= (F1-BP ) ×100 / BP.其中,AGR为50~170日龄的绝对增重率;W2-W1 为饲养50~170 d的体增重;t2-t1 为两阶段的间隔天数;F1为杂交一代某性状的平均值;BP为优良亲本某性状的平均值.体型分析利用体长/全长、头长/全长、体高/体长及体宽/体长4个比例参数进行比较.[结果]体重、体长及绝对增重率均为 F1> ZR> YR;体重、体长,在50日龄组合间差异不显著(P>0.05),在170日龄组合间差异均极显著(P<0.01);绝对增重率,F1分别比ZR、YR高20.00%、50.00%,F1与ZR差异不显著(P>0.05),与YR差异显著(P<0.05);F1在体重上表现明显的超亲杂种优势(20.09%).3个组合间,体长/全长差异不显著(P>0.05),头长/全长、体高/体长及体宽/体长差异显著(P<0.05),F1表现头短、体高、体宽等特点.[结论] F1在生长性能和体型特征上均具有优势.%[Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR (Yangtze River stock ♀× Yangtze River stock ♂), ZR (Zhujiang River stock ♀× Zhujiang River stock ♂) and hybrids F1 (Yangtze River stock ♀×Zhujiang River stock ♂) were

  2. Sterile Germination of the Hybrid Seeds of [ Den. Burana Green Star × Den. Rainbow-compactum ] and Rapid Propagation of F1 Plantlets via Tissue Culture%石斛兰"绿色星辰×出水芙蓉"杂交种子无菌萌发与组培快繁研究

    Institute of Scientific and Technical Information of China (English)

    符岸军; 李娟玲; 李劲松; 张勇; 刘国民

    2011-01-01

    [ Objective ] This study was to germinate the hybrid seeds of [ Den. Burana Green Star × Den. Rainbow-compactum ] under aseptic condition and to explore the parameters for rapid propagation of F1 plantlets via tissue culture. [ Method ] Hybridization between Den. Burana Green Star(female parent) and Den. Rainbow-compactum(male parent) was performed and the in vitro culture and proliferation of F1 hybrids were studied using eight different basic media including MS, 1/2MS, 1/3MS, 1/4MS, B5, N6, modified Knudson and H. [ Result] Improved Knudson medium appended with 1.00 mg/L 6-BA, 1.00 mg/L NAA and 10% mature banana puree performed best in F1 seed germination un der aseptic condition, as well as the rapid propagation of protocorm-like body. Of all the eight media tested, 1/2MS is the medium most suit able for the in vitro rapid propagation of the F1 seedlings. Efficiency of eight media in the in vitro rapid propagation was in order 1/2MS > MS > 1/3MS > 1/4MS ≈N6 > improved Knudson ≈B5 > H. NAA presented better roofing and growth-promoting effect in the in vitro rapid propagation of the F1 seedlings than IBA. And the optimal NAA concentration to recommend from our experiment results was 2.00 mg/L. [ Conclusion ] Our experimental results provided mature method and important technological information for hybrid breeding of dendrobium.%[目的]研究石斛兰"绿色星展×出水芙蓉"杂交种子无菌萌发与组培快繁.[方法]以"绿色星晨"为母本、"出水芙蓉"为父本进行杂交,并对其杂交种子进行了离体培养和组培快繁.[结果]石斛兰杂交种"绿色星辰×出水芙蓉"的F1代种子在改良Knudson培养基上(附加1.00 mg/L6-BA、1.00 mg/L NAA和10.0%熟香蕉泥)可获得理想的无菌萌发效果,且原球茎在谊培养基上也能较快增殖.以1/2MS最适合于该杂种石斛兰F1代无菌实生苗的组培快繁;8种供试培养基用于增殖培养的效果为:1/2MS>MS>1/3MS>1/4MS≈N6>改良Knudson≈B5>H

  3. UNDULATORY SWIMMING: HOW TRAVELING WAVES ARE PRODUCED AND MODULATED IN SUNFISH (LEPOMIS GIBBOSUS)

    Science.gov (United States)

    Long; Mchenry; Boetticher

    1994-07-01

    We have developed an experimental procedure in which the in situ locomotor muscles of dead fishes can be electrically stimulated to generate swimming motions. This procedure gives the experimenter control of muscle activation and the mechanical properties of the body. Using pumpkinseed sunfish, Lepomis gibbosus, we investigated the mechanics of undulatory swimming by comparing the swimming kinematics of live sunfish with the kinematics of dead sunfish made to swim using electrical stimulation. In electrically stimulated sunfish, undulatory waves can be produced by alternating left­right contractions of either all the axial muscle or just the precaudal axial muscle. As judged by changes in swimming speed, most of the locomotor power is generated precaudally and transmitted to the caudal fin by way of the skin and axial skeleton. The form of the traveling undulatory wave ­ as measured by tail-beat amplitude, propulsive wavelength and maximal caudal curvature ­ can be modulated by experimental control of the body's passive stiffness, which is a property of the skin, connective tissue and axial skeleton.

  4. 78 FR 60766 - Endangered and Threatened Wildlife and Plants; Threatened Species Status for Spring Pygmy Sunfish

    Science.gov (United States)

    2013-10-02

    ... from juried (peer-reviewed) professional journals whenever available. All decisions are made on the... commenter postulated that mechanical disturbance and siltation actually benefit the spring pygmy sunfish. He... the species weighed against the benefits provided through the current and proposed CCAAs. The...

  5. F1的学前班

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ 从CART比赛进入F1 美国一直缺少比F1更低级别的方程式比赛,美国(一些其它美洲国家也是这样)车手想要进入F1通常会去欧洲参加比赛或者在美国尝试与F1接近的CART赛车.

  6. F1‘Cherry Begonia’Series from Interspecific Hybridization of Begonia sempe rflorens×Begonia tuberhybrida%四季秋海棠与球根秋海棠远缘杂种——樱桃海棠

    Institute of Scientific and Technical Information of China (English)

    张成敏; 孙卫邦; 张石宝; 郭瑞贤; 魏兆祥; 郑若仙

    2001-01-01

    用粉红色的四季秋海棠为母本与红色的球根秋海棠进行远缘杂 交,获得了F1代杂种。由F1代中筛选出了3个高度重瓣、花朵如串串樱桃垂吊的樱桃海 棠品系。其花朵硕大、色彩艳丽,既有母本适应性强、花期长、繁殖栽培容易和不易倒伏等 特性,又因根茎部明显膨大,增强了抗寒性。%The new F1‘Cherry Begonia’series has been selected from the interspecific hybridization between Begonia semperflorens(maternal plant,pink)and Begonia tuberhybrida(red).The‘Cherry Begonia’series has h ighly pendent double-petals flowers hanging on the flower-spikes.It not only h as characteristics of the stronger adaptability,long florescence and easy to pr opagate and cultivation as same as the maternal plant,but also it has the brigh t and bigger flowers,more flowers on each inflorescence and richer flower color range(red,pink and white)in comparison with Begonia semperflorens.

  7. 苹果杂交F1代丰产性状综合选择模式研究%Study on comprehensive selection of hybrid seedlings characters of high yield breeding in apple

    Institute of Scientific and Technical Information of China (English)

    王雷存; 樊红科; 赵政阳; 高华

    2010-01-01

    [目的]分析苹果杂交后代单株产量与单果质量、坐果率、叶面积、干粗、果实发育期、叶绿素含量、净光合速率、百叶质量之间的关联程度.[方法]以"短枝富士"和"粉红女士"多年的性状平均表现和预期育种目标为基础,制订其杂交后代主要性状的分级标准,通过对261株短枝富士与粉红女士杂交F1代单果质量、坐果率、叶面积、干粗、果实发育期、叶绿素含量、净光合速率和百叶质量等的调查,计算产量与各性状的相关程度,建立丰产性状的综合选择模式,并对该模式进行了验证.[结果]杂交后代单株产量与单果质量、坐果率、干粗、净光合速率、百叶质量显著相关;与叶面积、叶绿素含量、果实发育期相关性不显著.选取与单株产量相关性显著的5个性状,结合果树育种实际,建立了一套苹果杂交F1代丰产性状综合评价的数量化模式.[结论]丰产性状综合选择模式在苹果育种实践中具有良好的实用性.

  8. Phenotypic variation of F1 and F2 populations from three species of ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-07-18

    Jul 18, 2008 ... Phenotypic variation of F1 and F2 populations from ... Key words: Solanum, genome, phenotype, taxonomy, evolution, interspecific hybridization, pollen viability, ..... This development affirms the views of .... The origins of.

  9. Growth, life history, and species interactions of bluegill sunfish (Lepomis macrochirus) under heavy predation

    Energy Technology Data Exchange (ETDEWEB)

    Belk, M.C. [Georgia Univ., Athens, GA (United States)

    1992-12-31

    The purpose of this study was, first, to compare growth and life history characteristics of an unfished population of bluegill sunfish (Lepomis macrochirus) in the presence of an abundant predator population to characteristic exhibited by bluegills in typical southeastern US reservoirs where the abundance of predators is reduced, but fishing is increased. The second objective was to determine if differences observed between populations were determined genetically or environmentally.

  10. The Correlation of Upwelling Phenomena and Ocean Sunfish Occurrences in Nusa Penida, Bali

    Science.gov (United States)

    Tito, C. K.; Susilo, E.

    2017-02-01

    Sea surface Temperature (SST) is an important oceanographic variable that can figure the upwelling phenomena. This study aims to determine the variability of SST in relation to upwelling phenomena in the Indian Ocean Southern of Bali Island and the Ocean Sunfish occurrences in the southern of Nusa Penida. Data loggers and remote sensing approach that record temperature was used. An Onset HOBO U20 Water Level Logger U20-001-02 was deployed in Crystal Bay (08°42‧S and 115°27‧E) at 8 meters depth. The daily field SST data were available from June 2011 to December 2014 with 30 minutes time interval. The monthly satellite images obtained from MODIS on board the Aqua satellite. While the ocean sunfish occurrences were based on rate of encounter (ROE) of previous works by Putra (2015) on July to October 2014. It was found that field data and MODIS have a high correlation (r=0.89) with Root Mean Square Error (RMSE=1.64°C). The upwelling phenomena characterized by the evidence of the colder water mass (SST phenomena coincidence with the high occurrences of Ocean Sunfish in Crystal Bay on August to October.

  11. Satellite Tracking and Site Fidelity of Short Ocean Sunfish, Mola ramsayi, in the Galapagos Islands

    Directory of Open Access Journals (Sweden)

    Tierney M. Thys

    2017-01-01

    Full Text Available Ocean sunfishes, with their peculiar morphology, large size, and surface habits, are valuable assets in ecotourism destinations worldwide. This study investigates site fidelity and long-range movements of short ocean sunfish, Mola ramsayi (Giglioli 1883, at Punta Vicente Roca (PVR off Isabela Island in the Galapagos Islands. Five individuals were tracked between 32 and 733 days using ultrasonic receivers and transmitters. Two of the 5 were also tracked with towed pop-off satellite tags. One travelled to the equatorial front covering 2700 km in 53 days, with dive depths in the upper 360 m at temperatures between 9.2°C and 22°C. During its westward travel, dives extended to 1112 m (the deepest depth yet recorded for Molidae into temperatures ranging between 4.5°C and 23.2°C. The remaining four individuals demonstrated site fidelity to PVR and were detected at the site between 128–1361 times for a total of 3557 reports. Forty-eight percent of the reports occurred during daytime hours and 52% after dark. Presumed cleaning session durations had a median of 15 minutes and a maximum of nearly 100 minutes. No other ultrasonic arrays around Galapagos or in the Eastern Pacific regional network recorded the presence of tagged individuals. These data are combined with tourist vessel sightings and submersible observations to confirm Punta Vicente Roca as an important sunfish hotspot.

  12. Main: E2F1OSPCNA [PLACE

    Lifescience Database Archive (English)

    Full Text Available E2F1OSPCNA S000396 21-May-2002 (last modified) uchi re2f-1 found in the promoter of rice PCN...ividing cells and tissue; E2F; PCNA; meristematic tissue; cell cycle; rice (Oryza sativa); tobacco (Nicotiana tabacum) GCGGGAAA ...

  13. 番茄设施栽培专用新品种晋番茄6号的选育%Breeding of New Tomato F1 Hybrid for Greenhouses Jinfanqie 6

    Institute of Scientific and Technical Information of China (English)

    王永珍; 张剑国

    2011-01-01

    Jinfanqie 6 is a new tomato hybrid with parental lines of D65-62 and N98-3. Its plant is indeterminate with high resistance to ToMV , medium resistance to CMV and complex resistance to some main diseases. Its ripe fruits are oblate, red and less deformed of good quality, long shelf life, fitting for transportation as well as good market ability. Single fruit ranges 180 to 250 gram. Its yield is more than 90 000 kg/hm2. It could be cultivated in greenhouses.%晋番茄6号是用自交系D65-62作母本、N98-3作父本配制的一代杂种,属无限生长类型,中熟,高抗番茄花叶病毒,中抗黄瓜花叶病毒,复合抗病性强.其果实高扁,成熟果大红色,耐贮运,单果质量180~250 g,不易产生畸裂果,商品性好,一般产量在90 000 kg/hm2以上.适于温室大棚栽培.

  14. A New Mini Chinese Cabbage F1 Hybrid-‘Jingchunwa No. 3’%小株型大白菜新品种京春娃3号的选育

    Institute of Scientific and Technical Information of China (English)

    余阳俊; 张凤兰; 张德双; 赵岫云; 于拴仓; 徐家炳; 汪维红; 苏同兵

    2015-01-01

    ‘Jingchunwa No. 3’ is a new mini Chinese cabbage hybrid bred by crossing cytoplasmic male sterile line CMS10152 with self imcompatible line 09219. It has flat-topped head and deep yellow inner leaves. It takes about 55 days from transplanting to harvest. It is of small plant type. The plant is erect with dark green outer leaf. Its head shape is cylindrical and flat-topped with 21.9 cm of head height and 10.2 cm of head diameter. Its inner leaves are deep yellow in color. Its single head weight is 0.7 kg. The yield in open field is about 90-105 t·hm-2. It is resistant to virus, downy mildew and black rot. Its tolerance to late bolting is strong. Its quality is good. It fits to transplant in high density of 10 000-12 000 plants per 667 m2. It has been extended to Beijing, and Hebei, Gansu, Yunnan, Hubei Provinces.%京春娃3号是由细胞质雄性不育系CMS10152与自交不亲和系09219配制而成的叠抱型深黄心小株型大白菜一代杂种。早熟,定植后55 d(天)收获,株型小,较直立,外叶深绿色,叶球筒形,叠抱,球内叶深黄色,球高21.9 cm,球直径10.2 cm,单球质量0.7 kg,667 m2产净菜6000~7000 kg。抗病毒病、霜霉病和黑腐病,耐抽薹性较强,品质佳。适于密植,每667 m2可定植10000~12000株。已在北京、河北、甘肃、云南、湖北等地推广种植。

  15. A New Superior Early-maturing Tomato F1 Hybrid-‘ Bianfen 11'%早熟优质番茄新品种汴粉11的选育

    Institute of Scientific and Technical Information of China (English)

    赵新彬; 侯洪淼; 李艳玲; 赵振宇; 王金城; 陈随柱

    2012-01-01

    'Bianfen 11' is a new tomato Fi hybrid with early-maturity and excellent quality developed by crossing high hardness self-sterile inbred line ksl86 as female parent and inbred line ks105 as male parent. It is of indeterminate growth type, with strong growth vigor. It has high resistance to low temperature and weak light,good resistance to stress,and good continuous fruiting ability. Its fruit shape is round with smooth surface. The matured fruit is pink in color. The average single fruit weight is 216 g. The fruit firmness is high. It is tolerant to storage and transportation. The fructification is of consistency. The deformity rate and dehiscent fruit rate are very low and commodity ratio is high. The soluble solid content is 6.0%. The VC content is 157 mg·kg-1 . The lycopene content is 21.60 mg·kg-1 . It is highly resistant to ToMV, leaf mildew, Fusarium wilt and resistant to CMV. It is suitable to be cultivated in Henan, Hebei, Shanxi, Shandong Provinces, etc. for protected cultivation in early spring, after autumn or overwinter.%汴粉11是以高硬度优良自交系ks186作母本、自交系ks105作父本配制而成的早熟优质番茄一代杂种.无限生长类型,早熟,生长旺盛,耐低温弱光,抗逆性强,连续坐果能力极强;成熟果粉红色,高圆形,果面光滑,平均单果质量216 g;果实硬度高,耐贮运;果实均匀一致,畸形果和裂果率极低,商品率高;可溶性固形物含量6.0%,VC 157 mg·kg-1,番茄红素21.60 mg·kg-1,品质佳;高抗ToMV、叶霉病、枯萎病,抗CMV.适宜河南、河北、山西、山东等地早春、秋延、越冬设施栽培.

  16. Activation of Ftz-F1-Responsive Genes through Ftz/Ftz-F1 Dependent Enhancers

    Science.gov (United States)

    Field, Amanda; Xiang, Jie; Anderson, W. Ray; Graham, Patricia; Pick, Leslie

    2016-01-01

    The orphan nuclear receptor Ftz-F1 is expressed in all somatic nuclei in Drosophila embryos, but mutations result in a pair-rule phenotype. This was explained by the interaction of Ftz-F1 with the homeodomain protein Ftz that is expressed in stripes in the primordia of segments missing in either ftz-f1 or ftz mutants. Ftz-F1 and Ftz were shown to physically interact and coordinately activate the expression of ftz itself and engrailed by synergistic binding to composite Ftz-F1/Ftz binding sites. However, attempts to identify additional target genes on the basis of Ftz-F1/ Ftz binding alone has met with only limited success. To discern rules for Ftz-F1 target site selection in vivo and to identify additional target genes, a microarray analysis was performed comparing wildtype and ftz-f1 mutant embryos. Ftz-F1-responsive genes most highly regulated included engrailed and nine additional genes expressed in patterns dependent on both ftz and ftz-f1. Candidate enhancers for these genes were identified by combining BDTNP Ftz ChIP-chip data with a computational search for Ftz-F1 binding sites. Of eight enhancer reporter genes tested in transgenic embryos, six generated expression patterns similar to the corresponding endogenous gene and expression was lost in ftz mutants. These studies identified a new set of Ftz-F1 targets, all of which are co-regulated by Ftz. Comparative analysis of enhancers containing Ftz/Ftz-F1 binding sites that were or were not bona fide targets in vivo suggested that GAF negatively regulates enhancers that contain Ftz/Ftz-F1 binding sites but are not actually utilized. These targets include other regulatory factors as well as genes involved directly in morphogenesis, providing insight into how pair-rule genes establish the body pattern. PMID:27723822

  17. Revising the $f_1(1420)$ resonance

    CERN Document Server

    Debastiani, V R; Liang, Wei-Hong; Oset, E

    2016-01-01

    We have studied the production and decay of the $f_1(1285)$ into $\\pi a_0(980)$ and $K^* \\bar K$ as a function of the mass of the resonance and find a shoulder around 1400 MeV, tied to a triangle singularity, for the $\\pi a_0(980)$ mode, and a peak around 1420 MeV with about 60 MeV width for the $K^* \\bar K$ mode. Both these features agree with the experimental information on which the $f_1(1420)$ resonance is based. In addition, we find that if the $f_1(1420)$ is a genuine resonance, coupling mostly to $K^* \\bar K$ as seen experimentally, one finds unavoidably about a 20\\% fraction for $\\pi a_0(980)$ decay of this resonance, in drastic contradiction with all experiments. Altogether, we conclude that the $f_1(1420)$ is not a genuine resonance, but the manifestation of the $\\pi a_0(980)$ and $K^* \\bar K$ decay modes of the $f_1(1285)$ at higher energies than the nominal one.

  18. Reference: E2F1OSPCNA [PLACE

    Lifescience Database Archive (English)

    Full Text Available E2F1OSPCNA Kosugi S, Ohashi Y E2F sites that can interact with E2F proteins cloned from rice are require...d for meristematic tissue-specific expression of rice and tobacco proliferating cell nuclear antigen promoters Plant J 29: 45-59 (2002) PubMed: 12060226; ...

  19. A molecular genetic examination of the mating system of pumpkinseed sunfish reveals high pay-offs for specialized sneakers.

    Science.gov (United States)

    Rios-Cardenas, Oscar; Webster, Michael S

    2008-05-01

    Intrasexual variation in reproductive behaviour and morphology are common in nature. Often, such variation appears to result from conditional strategies in which some individuals (e.g. younger males or those in poor condition) adopt a low pay-off phenotype as a 'best of a bad job'. Alternatively, reproductive polymorphisms can be maintained by balancing selection, with male phenotypes having equal fitnesses at equilibrium, but examples from nature are rare. Many species of sunfish (genus Lepomis) are thought to have alternative male reproductive behaviours, but most empirical work has focused on the bluegill sunfish and the mating systems of other sunfish remain poorly understood. We studied a population of pumpkinseed sunfish (Lepomis gibbosus) in upstate New York. Field observations confirm the existence of two male reproductive strategies: 'parentals' were relatively old and large males that maintained nests, and 'sneakers' were relatively young and small males that fertilize eggs by darting into nests of parentals during spawning. The sneaker and parental male strategies appear to be distinct life-history trajectories. Sneaker males represented 39% of the males observed spawning, and sneakers intruded on 43% of all mating attempts. Microsatellite analyses revealed that sneaker males fertilized an average of 15% of the eggs within a nest. This level of paternity by sneaker males appears to be higher than seen in most other fishes, and preliminary analyses suggest that the two male reproductive strategies are maintained as a balanced polymorphism.

  20. Long term patterns in the late summer trophic niche of the invasive pumpkinseed sunfish Lepomis gibbosus

    Directory of Open Access Journals (Sweden)

    Gkenas C.

    2016-01-01

    Full Text Available Quantifying the trophic dynamics of invasive species in novel habitats is important for predicting the success of potential invaders and evaluating their ecological effects. The North American pumpkinseed sunfish Lepomis gibbosus is a successful invader in Europe, where it has caused negative ecological effects primarily through trophic interactions. Here, we quantified variations in the late summer trophic niche of pumpkinseed during establishment and integration in the mainstem of the Guadiana river, using stomach content analyses over a period of 40 years. Pumpkinseed showed a shift from trophic specialization during establishment to trophic generalism during integration. These results were concomitant with an increase in diet breadth that was accompanied by higher individual diet specialization particularly in large individuals. Irrespective of their drivers, these changes in trophic niche suggest that the potential ecological effects of pumpkinseed on recipient ecosystems can vary temporally along the invasion process.

  1. Correlation Between Parents and F1 and Combining Ability of Parents on Seed Dormancy in indica Rice (Oryza sativa)

    OpenAIRE

    Xu, Bao-Qin; Zuo-mei LU

    2009-01-01

    Dormancy indices of hulled and dehulled seeds were investigated by using 19 cytoplasmic male sterile (CMS) lines, 9 restorer lines and their 109 F1 hybrids of indica hybrid rice. The seeds of each F1 and the parents were harvested on 35 days after flowering. Combining ability was analyzed in 25 combinations made by 5 CMS lines and 5 restorer lines (North Carolina II mating design). The seed dormancy index of F1 was positively and highly significantly correlated with those of their parents and...

  2. Analysis of the Current Pebrine Sampling Inspection of Mother Moth for F1 Hybrid with Hypergeometric Distri-bution Model and Suggest Improvement%现行一代杂交种母蛾微粒子病抽样检验方案的特点及改进意见

    Institute of Scientific and Technical Information of China (English)

    汤庆坤; 何国玲; 黄旭华; 胡文娟; 韦廷秀; 黄景滩

    2014-01-01

    Analyzed on the characteristics of the current pebrine inspection of mother moth of F1 Hybrid, and proposed some suggest improvements about the pebrine inspection of mother moth of F1 Hybrid which suit for the silkworm eggs on card.Used the hypergeometric distribution as the basic mathematical model, to make compara-tive analysis of the current pebrine inspection of mother moth of F1 Hybrid with sampling inspection principle. There are some advantages for agricultural industry standard of silkworm loose eggs that the unqualified batch are rejected with high probability and less inspection works, but it also has some disadvantages that the qualified batch are rejected with high probability, the risk of manufacturer are great. Another standard is agricultural in-dustry standard of silkworm eggs on card, it has some advantages, including that the sampling are simple and practicable, the sample are typical, the unit is unified, the unqualified batch of silkworm eggs are rejected in the right number, and there also are some disadvantages that the inspection probability has no guarantee, the probability of “two types of errors” are coexist, workload of inspection are heavy. The regional industry standard of Guangxi has the features of agricultural industry standard of silkworm loose eggs, but also has some disadvantages that the implementation of standard is not asymmetric, the sample is not typical and the risk of manufacturer is further increased. Therefore, we put forward a fitting scheme and suggestions for actual produc-tion of silkworm eggs on card, including that stratified sampling, testing two times, controlled inspection risk, increased individual number in sampling group, groups as unit and the 1% index of percentage of dis-eased moths, etc.%分析现行桑蚕一代杂交种母蛾微粒子病检验方案的特点,提出适合平附种的母蛾微粒子病检验方案的改进意见。以超几何分布为基本数学模型,从抽样检验原理对桑

  3. Induction og 2n gametes for overcoming F1-sterility in lily and tulip

    NARCIS (Netherlands)

    Barba Gonzalez, R.; Miller, C.T.; Ramanna, M.S.; Tuyl, van J.M.

    2006-01-01

    For overcoming F1-sterility in interspecific hybrids, mitotic and meiotic polyploidisation is applied in lily and can result in fertile allopolyploids. The mechanism of viable pollen production of mitotic and meiotic polyploidisation is quite different. Mitotic polyploids are obtained by artificial

  4. Rooting pattern and nitrogen uptake of three cauliflower (Brassica oleracea var. botrytis) F1-hbrids

    NARCIS (Netherlands)

    Rather, K.; Schenk, M.K.; Everaarts, A.P.; Vethman, S.

    2000-01-01

    In a two-year field trial at the sites Ruthe (Germany, loess soil, Orthic Luvisol) and Schermer (The Netherlands, marine clay soil, Eutric Fluvisol) the cauliflower F1-hybrids Marine, Lindurian and Linford were compared in their efficiency of N use from limiting and optimum supplies of N. Limiting N

  5. Analysis list: Pou3f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pou3f1 Pluripotent stem cell + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou3f1....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou3f1.5.tsv http://dbarchive.biosc...iencedbc.jp/kyushu-u/mm9/target/Pou3f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pou3f1.Plu

  6. Analysis list: Pou5f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pou5f1 Embryonic fibroblast,Pluripotent stem cell + mm9 http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/target/Pou5f1.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou5f1.5.tsv h...ttp://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou5f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pou5f1....Embryonic_fibroblast.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pou5f1

  7. Charmless hadronic $B \\to (f_1(1285),f_1(1420)) P$ decays in the perturbative QCD approach

    CERN Document Server

    Liu, Xin; Li, Jing-Wu; Zou, Zhi-Tian

    2014-01-01

    We study twenty charmless hadronic $B \\to f_1 P$ decays, with $f_1$ representing axial-vector mesons $f_1(1285)$ and $f_1(1420)$ that resulting from a mixing of quark-flavor $f_{1q}$ and $f_{1s}$ states with the angle $\\phi_{f_1}$, in the perturbative QCD(pQCD) formalism. The estimations of branching ratios and CP asymmetries of the considered $B \\to f_1 P$ decays are presented in the pQCD approach with $\\phi_{f_1} \\sim 24^\\circ$ from recently measured $B_{d/s} \\to J/\\psi f_1(1285)$ decays. It is found that (a) the tree dominant $B^+ \\to f_1 \\pi^+$ and the penguin dominant $B^+ \\to f_1 K^+$ decays with large branching ratios[${\\cal O}(10^{-6})$] and large direct CP violations(around $14\\% \\sim 28\\%$ in magnitude) simultaneously are believed to be clearly measurable at the LHCb and Super-B factory experiments; (b) the nearly pure penguin-dominated $B_d \\to f_1 K_S^0$ and $B_s \\to f_1 (\\eta, \\eta')$ modes with safely negligible tree pollution also have large decay rates in the order of $10^{-6} \\sim 10^{-5}$, w...

  8. Immunohistochemical Localization of Prolactin Releasing Peptide in Hypothalamus-pituitary-ovary of the Young Murrah Hybrid Buffallo F1%催乳素释放肽在青年摩杂一代水牛下丘脑-垂体-卵巢轴的免疫组化定位

    Institute of Scientific and Technical Information of China (English)

    秦津; 谢莹雪; 王秋华; 宋小白; 李瑞明; 杨炳壮; 许典新

    2011-01-01

    ), ventromedialis nucleus ( VMN ) , ventrolateral nucleus ( VLN), dorsomedialis nucleus ( DMN) and dorsallateral nucleus (DLN). No PrRP immunoreactive positive fiber was found in supra-opticus nucleus(SON). In the pituitary, the PrRP immunoreactive positive cells could only be observed in adenohypophysial,but there was no PrRP immunoreactive positive fiber; there were a large number of PrRP immunoreactive positive fibers in neurohypophysis, but no PrRP immunoreactive positive cell. No PrRP immunoreactive positive product was found in the follicle and the mesenchyme of ovary,only few PRrP was found in corpus luteum. [Conclusion] The PrRP was widely distributed in hypothalamus-pituitary-ovary of the young Murrah hybrid buffalo F1.

  9. Phylogeography of the bluegill sunfish, Lepomis macrochirus, in the Mississippi River Basin.

    Science.gov (United States)

    Kawamura, Kouichi; Yonekura, Ryuji; Katano, Osamu; Taniguchi, Yoshinori; Saitoh, Kenji

    2009-01-01

    The Mississippi River Basin supports the richest fish fauna in eastern North America and has played a key role in the maintenance of fish biodiversity, especially as a refuge for freshwater fishes during glaciations. In this study, we investigated the phylogeography of the bluegill sunfish, Lepomis macrochirus, in eastern North America, using complete sequence of the mitochondrial ND1 gene from 369 samples collected at 15 sites. Phylogenetic analysis revealed two major lineages (northern and southern clades) in a parsimony network. A sympatric distribution of the lineages was widely observed in the Mississippi Basin. Sequence diversity in the two lineages was significantly lower in glaciated regions around the Great Lakes than in unglaciated regions. The two lineages were estimated to have diverged in the Kansan glaciation, and refugia for both existed around the Ouachita Highlands. The southern clade dispersed during the Yasmouth Interglacial, prior to the dispersal of the northern clade during the Sangamon Interglacial. In the northern clade, low genetic diversity and population fragmentation inferred by nested clade analysis (NCA) were considered due to bottleneck events in the Wisconsin glaciation, while the southern clade showed isolation by distance in a Mantel test. A difference in demographic fluctuation suggests that sympatry of the two lineages has resulted from recent secondary admixture through the range expansion of the northern clade in the post-Pleistocene. Large-scale admixture of multiple mtDNA lineages in L. macrochirus, which has not been recorded in other fishes in the Mississippi River Basin, may result from their high vagility.

  10. Brain Transcriptional Profiles of Male Alternative Reproductive Tactics and Females in Bluegill Sunfish

    Science.gov (United States)

    Partridge, Charlyn G.; MacManes, Matthew D.; Knapp, Rosemary; Neff, Bryan D.

    2016-01-01

    Bluegill sunfish (Lepomis macrochirus) are one of the classic systems for studying male alternative reproductive tactics (ARTs) in teleost fishes. In this species, there are two distinct life histories: parental and cuckolder, encompassing three reproductive tactics, parental, satellite, and sneaker. The parental life history is fixed, whereas individuals who enter the cuckolder life history transition from sneaker to satellite tactic as they grow. For this study, we used RNAseq to characterize the brain transcriptome of the three male tactics and females during spawning to identify gene ontology (GO) categories and potential candidate genes associated with each tactic. We found that sneaker males had higher levels of gene expression differentiation compared to the other two male tactics. Sneaker males also had higher expression in ionotropic glutamate receptor genes, specifically AMPA receptors, compared to other males, which may be important for increased spatial working memory while attempting to cuckold parental males at their nests. Larger differences in gene expression also occurred among male tactics than between males and females. We found significant expression differences in several candidate genes that were previously identified in other species with ARTs and suggest a previously undescribed role for cAMP-responsive element modulator (crem) in influencing parental male behaviors during spawning. PMID:27907106

  11. A biorobotic model of the sunfish pectoral fin for investigations of fin sensorimotor control

    Energy Technology Data Exchange (ETDEWEB)

    Phelan, Chris; Tangorra, James [Department of Mechanical Engineering, Drexel University, Philadelphia, PA 19104 (United States); Lauder, George [Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138 (United States); Hale, Melina, E-mail: tangorra@coe.drexel.ed [Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL 60637 (United States)

    2010-09-15

    A comprehensive understanding of the control of flexible fins is fundamental to engineering underwater vehicles that perform like fish, since it is the fins that produce forces which control the fish's motion. However, little is known about the fin's sensory system or about how fish use sensory information to modulate the fin and to control propulsive forces. As part of a research program that involves neuromechanical and behavioral studies of the sunfish pectoral fin, a biorobotic model of the pectoral fin and of the fin's sensorimotor system was developed and used to investigate relationships between sensory information, fin ray motions and propulsive forces. This robotic fin is able to generate the motions and forces of the biological fin during steady swimming and turn maneuvers, and is instrumented with a relatively small set of sensors that represent the biological lateral line and receptors hypothesized to exist intrinsic to the pectoral fin. Results support the idea that fin ray curvature, and the pressure in the flow along the wall that represents the fish body, capture time-varying characteristics of the magnitude and direction of the force created throughout a fin beat. However, none of the sensor modalities alone are sufficient to predict the propulsive force. Knowledge of the time-varying force vector with sufficient detail for the closed-loop control of fin ray motion will result from the integration of characteristics of many sensor modalities.

  12. Pectoral fins aid in navigation of a complex environment by bluegill sunfish under sensory deprivation conditions.

    Science.gov (United States)

    Flammang, Brooke E; Lauder, George V

    2013-08-15

    Complex structured environments offer fish advantages as places of refuge and areas of greater potential prey densities, but maneuvering through these environments is a navigational challenge. To successfully navigate complex habitats, fish must have sensory input relaying information about the proximity and size of obstacles. We investigated the role of the pectoral fins as mechanosensors in bluegill sunfish swimming through obstacle courses under different sensory deprivation and flow speed conditions. Sensory deprivation was accomplished by filming in the dark to remove visual input and/or temporarily blocking lateral line input via immersion in cobalt chloride. Fish used their pectoral fins to touch obstacles as they swam slowly past them under all conditions. Loss of visual and/or lateral line sensory input resulted in an increased number of fin taps and shorter tap durations while traversing the course. Propulsive pectoral fin strokes were made in open areas between obstacle posts and fish did not use the pectoral fins to push off or change heading. Bending of the flexible pectoral fin rays may initiate an afferent sensory input, which could be an important part of the proprioceptive feedback system needed to navigate complex environments. This behavioral evidence suggests that it is possible for unspecialized pectoral fins to act in both a sensory and a propulsive capacity.

  13. 26 CFR 1.415(f)-1 - Aggregating plans.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 5 2010-04-01 2010-04-01 false Aggregating plans. 1.415(f)-1 Section 1.415(f)-1...) INCOME TAXES Pension, Profit-Sharing, Stock Bonus Plans, Etc. § 1.415(f)-1 Aggregating plans. (a) In general. Except as provided in paragraph (g) of this section (regarding multiemployer plans), and taking...

  14. Resistance to tomato yellow leaf curl Thailand virus,TYLCTHV-[2] from Solanum habrochaites accession ‘L06112’ in F1 and BC1F1 generations

    Directory of Open Access Journals (Sweden)

    Julapark Chunwongse4

    2008-07-01

    Full Text Available Resistance to tomato yellow leaf curl disease caused by Tomato yellow leaf curl Thailand virus (TYLCTHV-[2] inwild tomato, Solanum habrochaites ‘L06112’ was investigated. The ‘L06112’ accession expressing the resistant phenotypewas crossed to the TYLCV-susceptible female parent, Seedathip3, to produce F1 hybrids. Parental polymorphism and hybrididentity were tested using 12 pairs of microsatellite markers for each chromosome. All markers were polymorphic betweenthe parents, but only markers SSR46, SSR115, SSR117 and SSR128 gave results suitable to assess hybrid relationships.Polymorphic bands were sharp, concise and distinguishable between hybrids and selfed plants. The stem cuttings of donorand recurrent parents, their F1 and BC1F1 were inoculated with TYLCTHV-[2] using viruliferous whiteflies. Diseaseresponse of the plants was evaluated by Enzyme-Linked Immunosorbent Assay (ELISA at 45 days post inoculation. Thedonor parental line showed complete resistance to TYLCTHV-[2] while the F1 and BC1F1 expressed various ELISA readingsfor TYLCTHV-[2] concentration. BC1F1; 04T105-7, 04T105-1, 04T105-10, 04T109-4 and 04T104-1 developed from thisstudy showed the high level of resistance to TYLCV, Thailand isolate.

  15. The Resistance of Chinese Wild Vitis to Uncinula necator and its Inheritance in F1 Generation

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jian-xia; WANG Yue-jin; XU Yan

    2002-01-01

    By natural field identification, the resistance of Chinese wild Vitis to Uncinula necator and its inheritance in F1 generation were studied with 35 clones of 9 Chinese wild Vitis species, 171 F1 individuals of 4 inter-species cross between Chinese wild Vitis and Vitis vinifera cultivars, and 16 individuals of selfpollinated Chinese wild Vitis. Results showed that the phenotypes of resistance to Uncinula necator in Chinese wild Vitis and its F1 generation were rich and diverse. Based on the segregation of resisitance to Uncinula necator in the progenies resulted from both interspecific hybridization and self-pollination, of Chinese native wild Vitis species and clones were controlled by polygenes showing dominant independent heredity. Minor resistant genes were also exist in Chinese wild susceptible Vitis species and clones.

  16. Analysis list: E4f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available E4f1 Embryonic fibroblast + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/E4f1....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/E4f1.5.tsv http://dbarchive.biosciencedb...c.jp/kyushu-u/mm9/target/E4f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/E4f1.Embryonic_fibr

  17. Analysis list: Gtf2f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Gtf2f1 Blood + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Gtf2f1.1.t...sv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Gtf2f1.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Gtf2f1....10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Gtf2f1.Blood.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Blood.gml ...

  18. Analysis list: Pou2f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Pou2f1 Blood + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou2f1.1.t...sv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou2f1.5.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Pou2...f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Pou2f1.Blood.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Blood.gml ...

  19. Observation and Comparison of Chloroplast Structure in Hybrid and Different Cytoplasmic Male-sterile Wheat Lines%不同细胞质小麦雄性不育系及杂种F1叶绿体的观察与比较

    Institute of Scientific and Technical Information of China (English)

    袁凯; 高庆荣; 张保雷; 于松; 李楠楠; 张磊; 刘桓; 杨志远; 付修义

    2012-01-01

    chloroplast micro-structural characters in CMS male-sterile lines. [Method] The experiment was conducted with cultivars of the K, V, T-type CMS lines, the common maintainer Ji 5418, the F1 hybrids and the cytoplasmic donors under field conditions. The transmission electron microscope was used to investigate the features and expression differences of the chloroplast microstructure of the flag leaf. [Result] The chloroplast structure of K, V, T-type CMS lines were anomalous and showed that the boundaries of grana lamella of the choloraplast of were fuzzy, even disappeared, and also lag behind normal development. Meanwhile, the thylakoids among grana lamella were under developed and even ruptured, with the whole lamella disordered. But the maintainer line(B) Ji 5418's chloroplast was in good condition and close to the intima, the grana lamella clear and in order, and the thylakoid among grana was clearly visible. The number of chloroplasts per cell of the maintainer line was significantly different from K- and V-type (F=40.47, Pr<0.0001), with 19.8 in K-type and 18.4 in T-type, respectively. Moreover, the K-and T-type CMS lines had round chloroplast. The number of chloroplasts per cell in CMS V-type is 24.8, which was observably higher than that of CMS K and T-type (F=40.47, iV<0.0001), but did not significantly differ from the maintainer line (F=40.47, Pr<0.0001), with the number of chloroplasts of 24.1. Besides, the oblong chloroplast was found in CMS V-type CMS line and maintainer line. The donors of Aegilops kotschyi and Triticum timopheevii had round chloroplast, and oblong in Aegilops ventricos. Their boundaries of grana lamella of the choloraplast were fuzzy, the thylakoids among grana lamella were under developed and even ruptured. That is similar to K-, V-, T-type CMS lines, and they have the same source. The three kinds of chloroplasts of (AXR) F1 were oblong and close to the cell intimal. Their bi-layer epicyte were clear and cytoplasm were dense. Meanwhile, the

  20. Urbanization is a major influence on microplastic ingestion by sunfish in the Brazos River Basin, Central Texas, USA.

    Science.gov (United States)

    Peters, Colleen A; Bratton, Susan P

    2016-03-01

    Microplastics, degraded and weathered polymer-based particles, and manufactured products ranging between 50 and 5000 μm in size, are found within marine, freshwater, and estuarine environments. While numerous peer-reviewed papers have quantified the ingestion of microplastics by marine vertebrates, relatively few studies have focused on microplastic ingestion by freshwater organisms. This study documents microplastic and manufactured fiber ingestion by bluegill (Lepomis macrochirus) and longear (Lepomis megalotis) sunfish (Centrarchidae) from the Brazos River Basin, between Lake Whitney and Marlin, Texas, USA. Fourteen sample sites were studied and categorized into urban, downstream, and upstream areas. A total of 436 sunfish were collected, and 196 (45%) stomachs contained microplastics. Four percent (4%) of items sampled were debris on the macro size scale (i.e. >5 mm) and consisted of masses of plastic, metal, Styrofoam, or fishing material, while 96% of items sampled were in the form of microplastic threads. Fish length was statistically correlated to the number of microplastics detected (p = 0.019). Fish collected from urban sites displayed the highest mean number of microplastics ingested, followed by downstream and upstream sites. Microplastics were associated with the ingestion of other debris items (e.g. sand and wood) and correlated to the ingestion of fish eggs, earthworms, and mollusks, suggesting that sunfish incidentally ingest microplastics during their normal feeding methods. The high frequency of microplastic ingestion suggest that further research is needed to determine the residence time of microplastics within the stomach and gut, potential for food web transfer, and adverse effects on wildlife and ecosystemic health. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Pig, F1 (wild boar x pig) and wild boar meat quality

    OpenAIRE

    Ragni, M.; S. Tarricone; Pinto, F.; Dimatteo, S; G. Marsico; Rasulo, A

    2010-01-01

    Sixteen carcasses of wild boars, pigs, hybrids F1 (wild boar x pig) and reared wild boar have been examined to study the meat quality and the fatty acid composition. Four carcasses came from hunted wild boars and twelve from animals reared in outdoor pens till nine months of age. The meat produced by the hunted wild animals, although not marketable, offers the best quality and nutritional characteristics. The use of hybrids reared in outdoor pens can approximate or equalize the hunted wild bo...

  2. The effects of acute temperature change on swimming performance in bluegill sunfish Lepomis macrochirus.

    Science.gov (United States)

    Jones, Emily A; Jong, Arianne S; Ellerby, David J

    2008-05-01

    Many fish change gait within their aerobically supported range of swimming speeds. The effects of acute temperature change on this type of locomotor behavior are poorly understood. Bluegill sunfish swim in the labriform mode at low speeds and switch to undulatory swimming as their swimming speed increases. Maximum aerobic swimming speed (U(max)), labriform-undulatory gait transition speed (U(trans)) and the relationships between fin beat frequency and speed were measured at 14, 18, 22, 26 and 30 degrees C in bluegill acclimated to 22 degrees C. At temperatures below the acclimation temperature (T(a)), U(max), U(trans) and the caudal and pectoral fin beat frequencies at these speeds were reduced relative to the acclimation level. At temperatures above T(a) there was no change in these variables relative to the acclimation level. Supplementation of oxygen levels at 30 degrees C had no effect on swimming performance. The mechanical power output of the abductor superficialis, a pectoral fin abductor muscle, was measured in vitro at the same temperatures used for the swimming experiments. At and below T(a), maximal power output was produced at a cycle frequency approximately matching the in vivo pectoral fin beat frequency. At temperatures above T(a) muscle power output and cycle frequency could be increased above the in vivo levels at U(trans). Our data suggest that the factors triggering the labriform-undulatory gait transition change with temperature. Muscle mechanical performance limited labriform swimming speed at T(a) and below, but other mechanical or energetic factors limited labriform swimming speed at temperatures above T(a).

  3. Analysis list: E2f1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available E2f1 Blood,Liver + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/E2f1.1....tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/E2f1.5.tsv http://dbarchive.biosciencedbc.jp/kyus...hu-u/mm9/target/E2f1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/E2f1.Blood.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/colo/E2f1.Liver.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/mm9/colo/Blood.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Liver.gml ...

  4. Analysis of F1F0-ATPase from Helicobacter pylori.

    OpenAIRE

    1997-01-01

    The adaptive mechanisms that permit Helicobacter species to survive within the gastric mucosa are not well understood. The proton-translocating F1F0-ATPase is an important enzyme for regulating intracellular pH or synthesizing ATP in many other enteric bacteria; therefore, we used degenerate primers derived from conserved bacterial F1F0-ATPase sequences to PCR amplify and clone the gene (atpD) encoding the H. pylori F1F0-ATPase beta subunit. The deduced amino acid sequences of the F1F0-ATPase...

  5. Analysis list: POU5F1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available POU5F1 Epidermis,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyu...archive.biosciencedbc.jp/kyushu-u/hg19/target/POU5F1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/POU5F1.Epidermis...U5F1.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Epidermis.gml,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Pluripotent_stem_cell.gml ...

  6. Fathead minnow and bluegill sunfish life-stage responses to 17β-estradiol exposure in outdoor mesocosms

    Science.gov (United States)

    Elliott, Sarah M.; Kiesling, Richard L.; Jorgenson, Zachary G.; Rearick, Daniel C.; Schoenfuss, Heiko L.; Fredricks, Kim T.; Gaikowski, Mark P.

    2014-01-01

    Developmental and reproductive effects of 17β-estradiol (E2) exposure on two generations of fathead minnows and one generation of bluegill sunfish were assessed. Fish were exposed to E2 for six continuous weeks in outdoor mesocosms simulating natural lake environments. First generation fish were exposed while sexually mature. Second generation fathead minnows were exposed either during early development, sexual maturity, or both stages. Multiple endpoints were measured to assess effects of E2 exposure on fecundity and fish health and development. Plasma vitellogenin concentrations were highly variable in all fish. Differences in egg production timing for both species indicate differences in fecundity between females exposed to E2 and controls. First generation fathead minnows exposed to E2 had lower body condition factors and reduced secondary sexual characteristic expression by males. Only a difference in relative liver weight was observed in second generation fathead minnows. First generation bluegill males exposed to E2 had significantly smaller testes compared to controls. Although fish response was highly variable, results indicate that exposure to E2 at environmentally relevant concentrations affect fathead minnow and bluegill sunfish health and development, which may have implications for the health and sustainability of fish populations. Furthermore, exposure timing and environmental factors affect fish response to E2 exposure.

  7. Morphological change and phenotypic plasticity in native and non-native pumpkinseed sunfish in response to competition

    Science.gov (United States)

    Yavno, Stan; Rooke, Anna C.; Fox, Michael G.

    2014-06-01

    Non-indigenous species are oftentimes exposed to ecosystems with unfamiliar species, and organisms that exhibit a high degree of phenotypic plasticity may be better able to contend with the novel competitors that they may encounter during range expansion. In this study, differences in morphological plasticity were investigated using young-of-year pumpkinseed sunfish ( Lepomis gibbosus) from native North American and non-native European populations. Two Canadian populations, isolated from bluegill sunfish ( L. macrochirus) since the last glaciation, and two Spanish populations, isolated from bluegill since their introduction in Europe, were reared in a common environment using artificial enclosures. Fish were subjected to allopatric (without bluegill) or sympatric (with bluegill) conditions, and differences in plasticity were tested through a MANOVA of discriminant function scores. All pumpkinseed populations exhibited dietary shifts towards more benthivorous prey when held with bluegill. Differences between North American and European populations were observed in body dimensions, gill raker length and pelvic fin position. Sympatric treatments induced an increase in body width and a decrease in caudal peduncle length in native fish; non-native fish exhibited longer caudal peduncle lengths when held in sympatry with bluegill. Overall, phenotypic plasticity influenced morphological divergence less than genetic factors, regardless of population. Contrary to predictions, pumpkinseeds from Europe exhibited lower levels of phenotypic plasticity than Canadian populations, suggesting that European pumpkinseeds are more canalized than their North American counterparts.

  8. Interspecific introgression in cetaceans: DNA markers reveal post-F1 status of a pilot whale.

    Directory of Open Access Journals (Sweden)

    Laura Miralles

    Full Text Available Visual species identification of cetacean strandings is difficult, especially when dead specimens are degraded and/or species are morphologically similar. The two recognised pilot whale species (Globicephala melas and Globicephala macrorhynchus are sympatric in the North Atlantic Ocean. These species are very similar in external appearance and their morphometric characteristics partially overlap; thus visual identification is not always reliable. Genetic species identification ensures correct identification of specimens. Here we have employed one mitochondrial (D-Loop region and eight nuclear loci (microsatellites as genetic markers to identify six stranded pilot whales found in Galicia (Northwest Spain, one of them of ambiguous phenotype. DNA analyses yielded positive amplification of all loci and enabled species identification. Nuclear microsatellite DNA genotypes revealed mixed ancestry for one individual, identified as a post-F1 interspecific hybrid employing two different Bayesian methods. From the mitochondrial sequence the maternal species was Globicephala melas. This is the first hybrid documented between Globicephala melas and G. macrorhynchus, and the first post-F1 hybrid genetically identified between cetaceans, revealing interspecific genetic introgression in marine mammals. We propose to add nuclear loci to genetic databases for cetacean species identification in order to detect hybrid individuals.

  9. [Study of the effects of gamma-irradiation of common wheat F1 seeds using gliadins as genetic markers].

    Science.gov (United States)

    Kozub, N O; Sozinov, I O; Blium, Ia B; Sozinov, O O

    2013-01-01

    Effects of irradiation of dry F1 seeds with gamma rays in the dose of 200 Gy were studied. Hybrids between near-isogenic lines on the basis of the variety Bezostaya 1 served as the material of investigation. Irradiation markedly reduced productivity traits of F1 plants and did not affect the survival of F1 plants under the given growth conditions. A significant relative increase in the frequency of pollen grains with the 1BL/1RS translocation that formed F2 seeds in comparison with the control was one of the effects of irradiation of F1 seeds. Irradiation with gamma-rays induced mutations at gliadin loci with the frequency of 7,4 % (at 0,5 % in the control).

  10. Correlation Between Parents and F1 and Combining Ability of Parents on Seed Dormancy in indica Rice (Oryza sativa)

    Institute of Scientific and Technical Information of China (English)

    XU Bao-qin; LU Zuo-mei

    2009-01-01

    Dormancy indices of hulled and dehulled seeds were investigated by using 19 cytoplasmic male sterile (CMS) lines, 9 restorer lines and their 109 F1 hybrids of indica hybrid rice. The seeds of each F1 and the parents were harvested on 35 days after flowering. Combining ability was analyzed in 25 combinations made by 5 CMS lines and 5 restorer lines (North Carolina II mating design). The seed dormancy index of F1 was positively and highly significantly correlated with those of their parents and mid-parent value. Out of the 109 combinations, 82 combinations showed mid-parent heterosis, and 43 heterobeltiosis. Seed dormancy indices of F1s and their parents declined dramatically in dehulled seeds compared with hulled seeds, indicating that the hull played an important role in seed dormancy. However, the trends were similar in hulled seeds and dehulled seeds in terms of relationships between the seed dormancy indicices in F1 and their parents. The influence of hull on seed dormancy mainly depended on F1 genotype, not on the hull from maternal parent. The variances of general combining ability (GCA) in female and male parents occupied 59.2% and 31.1% of total variance, respectively. The variance of specific combining ability (SCA) in combinations occupied 9.7% of total variance, indicating that gene additive effects were principal. Among the 5 CMS lines, II112A had the highest GCA effect for seed dormancy, followed by D62A. Among the 5 restorer lines, IR112 had the highest GCA effect for seed dormancy, followed by 2786. These lines are elite parental materials for breeding F1 hybrid rice with stronger seed dormancy.

  11. Correlation Between Parents and F1 and Combining Ability of Parents on Seed Dormancy in indica Rice (Oryza sativa

    Directory of Open Access Journals (Sweden)

    Bao-qin XU

    2009-03-01

    Full Text Available Dormancy indices of hulled and dehulled seeds were investigated by using 19 cytoplasmic male sterile (CMS lines, 9 restorer lines and their 109 F1 hybrids of indica hybrid rice. The seeds of each F1 and the parents were harvested on 35 days after flowering. Combining ability was analyzed in 25 combinations made by 5 CMS lines and 5 restorer lines (North Carolina II mating design. The seed dormancy index of F1 was positively and highly significantly correlated with those of their parents and mid-parent value. Out of the 109 combinations, 82 combinations showed mid-parent heterosis, and 43 heterobeltiosis. Seed dormancy indices of F1s and their parents declined dramatically in dehulled seeds compared with hulled seeds, indicating that the hull played an important role in seed dormancy. However, the trends were similar in hulled seeds and dehulled seeds in terms of relationships between the seed dormancy indicices in F1 and their parents. The influence of hull on seed dormancy mainly depended on F1 genotype, not on the hull from maternal parent. The variances of general combining ability (GCA in female and male parents occupied 59.2% and 31.1% of total variance, respectively. The variance of specific combining ability (SCA in combinations occupied 9.7% of total variance, indicating that gene additive effects were principal. Among the 5 CMS lines, II112A had the highest GCA effect for seed dormancy, followed by D62A. Among the 5 restorer lines, IR112 had the highest GCA effect for seed dormancy, followed by 2786. These lines are elite parental materials for breeding F1 hybrid rice with stronger seed dormancy.

  12. Localization of Magic-F1 Transgene, Involved in Muscular Hypertrophy, during Early Myogenesis

    Science.gov (United States)

    Ronzoni, Flavio; Bongio, Matilde; Conte, Silvio; Vercesi, Luigi; Cassano, Marco; Tribioli, Carla; Galli, Daniela; Bellazzi, Riccardo; Magenes, Giovanni; Cusella De Angelis, Maria Gabriella; Sampaolesi, Maurilio

    2011-01-01

    We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5 dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5–10.5–11.5 dpc) and cryostat sections for later stages (11.5–13.5–15.5–17.5 dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells. PMID:22187527

  13. Linkage analysis and map construction in genetic populations of clonal F1 and double cross.

    Science.gov (United States)

    Zhang, Luyan; Li, Huihui; Wang, Jiankang

    2015-01-15

    In this study, we considered four categories of molecular markers based on the number of distinguishable alleles at the marker locus and the number of distinguishable genotypes in clonal F1 progenies. For two marker loci, there are nine scenarios that allow the estimation of female, male, and/or combined recombination frequencies. In a double cross population derived from four inbred lines, five categories of markers are classified and another five scenarios are present for recombination frequency estimation. Theoretical frequencies of identifiable genotypes were given for each scenario, from which the maximum likelihood estimates of one or more of the three recombination frequencies could be estimated. If there was no analytic solution, then Newton-Raphson method was used to acquire a numerical solution. We then proposed to use an algorithm in Traveling Salesman Problem to determine the marker order. Finally, we proposed a procedure to build the two haploids of the female parent and the two haploids of the male parent in clonal F1. Once the four haploids were built, clonal F1 hybrids could be exactly regarded as a double cross population. Efficiency of the proposed methods was demonstrated in simulated clonal F1 populations and one actual maize double cross. Extensive comparisons with software JoinMap4.1, OneMap, and R/qtl show that the methodology proposed in this article can build more accurate linkage maps in less time.

  14. Progress of the NTSC-F1 primary frequency standard

    Institute of Scientific and Technical Information of China (English)

    RUAN; Jun; WANG; Xinliang; LIU; Dandan; GUAN; Yong; ZHANG; Hui; CHEN; Jiang; LIN; Rui; YU; Fengxiang; SHI; Junru; ZHANG; Shougang

    2015-01-01

    The SI "second"is realized by caesium primary frequency standards( PFSs) using laser cooled atoms in a fountain configuration. Four sub systems and operation procedure of the NTSC-F1 primary frequency standard are introduced in the paper.The frequency stability of NTSC-F1 is 3.0×10-13/ τ-1 / 2compared to hydrogen maser. Four terms of frequency shift and uncertainty including second order Zeeman frequency shift,cold collision shift,gravity shift and blackbody shift are evaluated. The improvement of NTSC-F1 is introduced.

  15. General formulae for f1 -> f2 γ

    Science.gov (United States)

    Lavoura, L.

    2003-07-01

    At one-loop level the decay f_1 to f_2 γ, where f1 and f2 are two spin-1/2 particles with the same electric charge, is mediated by a boson B and a spin-1/2 fermion F. The boson B may have either spin - interacting with the fermions through the Dirac matrices 1 and γ_5 - or spin 1 - with V+ A and V- A couplings to the fermions. I give general formulae for the one-loop electroweak amplitude of f_1 to f_2 γ in all these cases.

  16. General formulae for f1 --> f2 gamma

    CERN Document Server

    Lavoura, L

    2003-01-01

    At one-loop level the decay f1 --> f2 gamma, where f1 and f2 are two spin-1/2 particles with the same electric charge, is mediated by a boson B and a spin-1/2 fermion F. The boson B may have either spin 0 - interacting with the fermions through Dirac matrices 1 and gamma5 - or spin 1 - with V+A and V-A couplings to the fermions. I give general formulae for the one-loop electroweak amplitude of f1 --> f2 gamma in all these cases.

  17. Effects of Nonylphenol on Brain Gene Expression Profiles in F1 Generation Rats

    Institute of Scientific and Technical Information of China (English)

    YIN-YIN XIA; PING ZHANG; YANG WANG

    2008-01-01

    Objective To explore the effects of nonylphenol on brain gene expression profiles in F1 generation rats by microarray technique.Methods mRNA was extracted from the brain of 2-day old F1 generation male rats Whose F0 female generation was either exposed to nonylphenol or free from nonylphenol exposure,and then it was reversely transcribed to cDNA hbeled with cy5 and cy3 fluorescence.Subsequently,cDNA probes were hybridized to two BiostarR-40S cDNA gene chips and fluorescent signals of cy5 and cy3 were scanned and analyzed. Results Two genes were differentially down-regulated.Conclusion Nonylphenol may disturb the neurcendocrine function of male rats when administered perinatally.

  18. Cloning and Identification of Porcine SMPX Differentially Expressed in F1 Crossbreds and Their Parents

    Institute of Scientific and Technical Information of China (English)

    Zhu-Qing REN; Yuan-Zhu XIONG; Chang-Yan DENG; Ming-Gang LEI

    2006-01-01

    In order to investigate porcine heterosis on the molecular basis, Large White (L), a European purebred, and Meishan (M), a Chinese indigenous purebred, were hybridized directly and reciprocally to produce F1 hybrids, Large White×Meishan (LM) and Meishan×Large White (ML) pigs. Using mRNA differential display, we found an expression sequence tag (EST) differentially expressed in F1 hybrids and their parents, designated as EST55, which was homologous to human and murine skeletal muscle protein (SMPX), and the full-length cDNA of porcine SMPX was cloned by the rapid amplification of cDNA end (RACE) method. Translation of the mRNA transcript revealed an open reading frame (ORF) of 86 amino acid residues encoding a nuclear location signal peptide, two overlapping casein kinase Ⅱ phosphorylation sites and one N-glycosylation site with theoretical molecular weight of 9.3 kDa. Alignment analysis revealed that the deduced protein sequence shared 94%, 83% and 78% homology with that of its human, mouse and rat counterparts, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that it was expressed predominantly in skeletal and heart muscles, whereas at a moderate level in backfat,spleen, stomach and uterus tissues. Two single nucleotide polymorphism (SNPs), located in 5'- and 3'-untranslated region (UTR), respectively, were identified by PCR and sequencing. Phylogenetic tree and the secondary structure prediction were also performed. The possible relationship between porcine SMPX and heterosis was discussed.

  19. Integrating Bioengineered F1 Motors into Nano-Structured Surfaces

    Science.gov (United States)

    2013-04-23

    Cindy Berrie, Fei Gao. Insertion of a Rigid Structural Element into the Regulatory Domain of the Chloroplast F1-ATPase Gamma Subunit for Rotational...Studies., 15th International Photosynthesis Congress. 2010/08/22 01:00:00, . : , 12/27/2011 3.00 . The Mutation E242K in the chloroplast ATP synthase... chloroplast F1-ATPase gamma subunit for rotational studies. Proceedings of the 15th International Congress on Photosynthesis, 2011, pp.123-126. 2. Colvert

  20. Long-term GPS tracking of ocean sunfish Mola mola offers a new direction in fish monitoring.

    Directory of Open Access Journals (Sweden)

    David W Sims

    Full Text Available Satellite tracking of large pelagic fish provides insights on free-ranging behaviour, distributions and population structuring. Up to now, such fish have been tracked remotely using two principal methods: direct positioning of transmitters by Argos polar-orbiting satellites, and satellite relay of tag-derived light-level data for post hoc track reconstruction. Error fields associated with positions determined by these methods range from hundreds of metres to hundreds of kilometres. However, low spatial accuracy of tracks masks important details, such as foraging patterns. Here we use a fast-acquisition global positioning system (Fastloc GPS tag with remote data retrieval to track long-term movements, in near real time and position accuracy of <70 m, of the world's largest bony fish, the ocean sunfish Mola mola. Search-like movements occurred over at least three distinct spatial scales. At fine scales, sunfish spent longer in highly localised areas with faster, straighter excursions between them. These 'stopovers' during long-distance movement appear consistent with finding and exploiting food patches. This demonstrates the feasibility of GPS tagging to provide tracks of unparalleled accuracy for monitoring movements of large pelagic fish, and with nearly four times as many locations obtained by the GPS tag than by a conventional Argos transmitter. The results signal the potential of GPS-tagged pelagic fish that surface regularly to be detectors of resource 'hotspots' in the blue ocean and provides a new capability for understanding large pelagic fish behaviour and habitat use that is relevant to ocean management and species conservation.

  1. 翘嘴鳊及其亲本肌肉营养成分分析%Muscle nutrients of the backcross progeny of female diploid F1 hybrid (blunt snout bream × topmouth culter) × male blunt snout bream and its parents

    Institute of Scientific and Technical Information of China (English)

    何周玲; 刘少军; 肖军; 胡方舟; 文明; 叶利海; 张纯; 徐康; 陶敏

    2014-01-01

    为研究新型杂交鱼翘嘴鳊(二倍体鲂鲌F1♀×团头鲂♂)的肌肉营养价值,实验运用生化方法测定和分析了翘嘴鳊、翘嘴红鲌、团头鲂、二倍体鲂鲌F1(团头鲂早×翘嘴红鲌♂)的肌肉营养成分,包括这些样品的水分、脂肪、蛋白质和灰分含量,以及脂肪酸组成和氨基酸组成.结果表明,翘嘴鳊与其原始亲本相比具有较高的蛋白质和较低的碳水化合物含量;脂肪酸分析发现翘嘴鳊的不饱和脂肪酸,尤其是油酸、DHA等含量显著高于其原始父母本和其他有关鱼类(P<0.05);翘嘴鳊肌肉中具有较高呈味氨基酸比例,该比例高于其父母本的相应比例.研究表明,翘嘴鳊是一种营养价值高、口感好的优质鱼类.

  2. Nuclear receptor subfamily 2 group F member 1a (nr2f1a is required for vascular development in zebrafish.

    Directory of Open Access Journals (Sweden)

    Bao-Jueng Wu

    Full Text Available Genetic regulators and signaling pathways are important for the formation of blood vessels. Transcription factors controlling vein identity, intersegmental vessels (ISV growth and caudal vein plexus (CVP formation in zebrafish are little understood as yet. Here, we show the importance of the nuclear receptor subfamily member 1A (nr2f1a in zebrafish vascular development. Amino acid sequence alignment and phylogenetic analysis of nr2f1a is highly conserved among the vertebrates. Our in situ hybridization results showed nr2f1a mRNA is expressed in the lateral plate mesoderm at 18 somite stage and in vessels at 24-30 hpf, suggesting its roles in vasculization. Consistent with this morpholino-based knockdown of nr2fla impaired ISV growth and failed to develop fenestrated vascular structure in CVP, suggesting that nr2f1a has important roles in controlling ISV and CVP growth. Consequently, nr2f1a morphants showed pericardial edema and circulation defects. We further demonstrated reduced ISV cells and decreased CVP endothelial cells sprouting in nr2f1a morphants, indicating the growth impairment of ISV and CVP is due to a decrease of cell proliferation and migration, but not results from cell death in endothelial cells after morpholino knockdown. To test molecular mechanisms and signals that are associated with nr2f1a, we examined the expression of vascular markers. We found that a loss of nr2f1a results in a decreased expression of vein/ISV specific markers, flt4, mrc1, vascular markers stabilin and ephrinb2. This indicates the regulatory role of nr2f1a in controlling vascular development. We further showed that nr2f1a likely interact with Notch signaling by examining nr2f1a expression in rbpsuh morphants and DAPT-treatment embryos. Together, we show nr2f1a plays a critical role for vascular development in zebrafish.

  3. Further examination of seventeen mutations in Escherichia coli F1-ATPase beta-subunit.

    Science.gov (United States)

    Senior, A E; al-Shawi, M K

    1992-10-25

    Seventeen mutations in beta-subunit of Escherichia coli F1-ATPase which had previously been characterized in strain AN1272 (Mu-induced mutant) were expressed in strain JP17 (beta-subunit gene deletion). Six showed unchanged behavior, namely: C137Y; G142D; G146S; G207D; Y297F; and Y354F. Five failed to assemble F1F0 correctly, namely: G149I; G154I; G149I,G154I; G223D; and P403S,G415D. Six assembled F1F0 correctly, but with membrane ATPase lower than in AN1272, namely: K155Q; K155E; E181Q; E192Q; D242N; and D242V. AN1272 was shown to unexpectedly produce a small amount of wild-type beta-subunit; F1-ATPase activities reported previously in AN1272 were referable to hybrid enzymes containing both mutant and wild-type beta-subunits. Purified F1 was obtained from K155Q; K155E; E181Q; E192Q; and D242N mutants in JP17. Vmax ATPase values were lower, and unisite catalysis rate and equilibrium constants were perturbed to greater extent, than in AN1272. However, general patterns of perturbation revealed by difference energy diagrams were similar to those seen previously, and the new data correlated well in linear free energy relationships for reaction steps of unisite catalysis. Correlation between multisite and unisite ATPase activity was seen in the new enzymes. Overall, the data give strong support to previously proposed mechanisms of unisite catalysis, steady-state catalysis, and energy coupling in F1-ATPases (Al-Shawi, M. K., Parsonage, D. and Senior, A. E. (1990) J. Biol. Chem. 265, 4402-4410). The K155Q, K155E, D242N, and E181Q mutations caused 5000-fold, 4000-fold, 1800-fold, and 700-fold decrease, respectively, in Vmax ATPase, implying possibly direct roles for these residues in catalysis. Experiments with the D242N mutant suggested a role for residue beta D242 in catalytic site Mg2+ binding.

  4. Inheritance of steroid-independent male sexual behavior in male offspring of B6D2F1 mice.

    Science.gov (United States)

    McInnis, Christine M; Bonthuis, Paul J; Rissman, Emilie F; Park, Jin Ho

    2016-04-01

    The importance of gonadal steroids in modulating male sexual behavior is well established. Individual differences in male sexual behavior, independent of gonadal steroids, are prevalent across a wide range of species, including man. However, the genetic mechanisms underlying steroid-independent male sexual behavior are poorly understood. A high proportion of B6D2F1 hybrid male mice demonstrates steroid-independent male sexual behavior (identified as "maters"), providing a mouse model that opens up avenues of investigation into the mechanisms regulating male sexual behavior in the absence of gonadal hormones. Recent studies have revealed several proteins that play a significant factor in regulating steroid-independent male sexual behavior in B6D2F1 male mice, including amyloid precursor protein (APP), tau, and synaptophysin. The specific goals of our study were to determine whether steroid-independent male sexual behavior was a heritable trait by determining if it was dependent upon the behavioral phenotype of the B6D2F1 sire, and whether the differential expression of APP, tau, and synaptophysin in the medial preoptic area found in the B6D2F1 sires that did and did not mate after gonadectomy was similar to those found in their male offspring. After adult B6D2F1 male mice were bred with C57BL/6J female mice, they and their male offspring (BXB1) were orchidectomized and identified as either maters or "non-maters". A significant proportion of the BXB1 maters was sired only from B6D2F1 maters, indicating that the steroid-independent male sexual behavior behavioral phenotype of the B6D2F1 hybrid males, when crossed with C57BL/6J female mice, is inherited by their male offspring. Additionally, APP, tau, and synaptophysin were elevated in in the medial preoptic area in both the B6D2F1 and BXB1 maters relative to the B6D2F1 and BXB1 non-maters, respectively, suggesting a potential genetic mechanism for the inheritance of steroid-independent male sexual behavior. Copyright

  5. f(1)、f(0)、f(-1)表示f(x)=ax2+bx+c解竞赛题

    Institute of Scientific and Technical Information of China (English)

    严启国; 陈纯亮

    2003-01-01

    @@ 设函数f(x)=ax2+bx+c(-1≤x≤1),则f(1)=a+b+c,f(0)=c,f(-1)=a-b+c,解得a=1/2f(1)+1/2f(-1)-f(0),b=1/2f(1)-1/2f(-1),c=f(0),从而有f(x)=[1/2f(1)+1/2f(-1)-f(0)]x2+[1/2f(1)-1/2f(-1)]x+f(0),利用这一表示形式可以解下列竞赛题.

  6. M2-F1 on lakebed with pilot Milt Thompson

    Science.gov (United States)

    1963-01-01

    NASA Flight Research Pilot Milt Thompson, shown here on the lakebed with the M2-F1 lifting body, was an early backer of R. Dale Reed's lifting-body proposal. He urged Flight Research Center director Paul Bikle to approve the M2-F1's construction. Thompson also made the first glide flights in both the M2-F1 and its successor, the heavyweight M2-F2. The wingless, lifting body aircraft design was initially conceived as a means of landing an aircraft horizontally after atmospheric reentry. The absence of wings would make the extreme heat of re-entry less damaging to the vehicle. In 1962, NASA Flight Research Center (later Dryden Flight Research Center, Edwards, CA) management approved a program to build a lightweight, unpowered lifting body as a prototype to flight test the wingless concept. It would look like a 'flying bathtub,' and was designated the M2-F1, the 'M' referring to 'manned' and 'F' referring to 'flight' version. It featured a plywood shell placed over a tubular steel frame crafted at Dryden. Construction was completed in 1963. The first flight tests of the M2-F1 were over Rogers Dry Lake at the end of a tow rope attached to a hopped-up Pontiac convertible driven at speeds up to about 120 mph. This vehicle needed to be able to tow the M2-F1 on the Rogers Dry Lakebed adjacent to NASA's Flight Research Center (FRC) at a minimum speed of 100 miles per hour. To do that, it had to handle the 400-pound pull of the M2-F1. Walter 'Whitey' Whiteside, who was a retired Air Force maintenance officer working in the FRC's Flight Operations Division, was a dirt-bike rider and hot-rodder. Together with Boyden 'Bud' Bearce in the Procurement and Supply Branch of the FRC, Whitey acquired a Pontiac Catalina convertible with the largest engine available. He took the car to Bill Straup's renowned hot-rod shop near Long Beach for modification. With a special gearbox and racing slicks, the Pontiac could tow the 1,000-pound M2-F1 110 miles per hour in 30 seconds. It proved

  7. Robustness of the rotary catalysis mechanism of F1-ATPase.

    Science.gov (United States)

    Watanabe, Rikiya; Matsukage, Yuki; Yukawa, Ayako; Tabata, Kazuhito V; Noji, Hiroyuki

    2014-07-11

    F1-ATPase (F1) is the rotary motor protein fueled by ATP hydrolysis. Previous studies have suggested that three charged residues are indispensable for catalysis of F1 as follows: the P-loop lysine in the phosphate-binding loop, GXXXXGK(T/S); a glutamic acid that activates water molecules for nucleophilic attack on the γ-phosphate of ATP (general base); and an arginine directly contacting the γ-phosphate (arginine finger). These residues are well conserved among P-loop NTPases. In this study, we investigated the role of these charged residues in catalysis and torque generation by analyzing alanine-substituted mutants in the single-molecule rotation assay. Surprisingly, all mutants continuously drove rotary motion, even though the rotational velocity was at least 100,000 times slower than that of wild type. Thus, although these charged residues contribute to highly efficient catalysis, they are not indispensable to chemo-mechanical energy coupling, and the rotary catalysis mechanism of F1 is far more robust than previously thought.

  8. Supercongruences satisfied by coefficients of 2F1 hypergeometric series

    CERN Document Server

    Chan, Heng Huat; Krattenthaler, Christian; Osburn, Robert

    2009-01-01

    Recently, Chan, Cooper and Sica conjectured two congruences for coefficients of classical 2F1 hypergeometric series which also arise from power series expansions of modular forms in terms of modular functions. We prove these two congruences using combinatorial properties of the coefficients.

  9. Internal steel structure of M2-F1

    Science.gov (United States)

    1963-01-01

    The internal steel structure for the M2-F1 was built at the Flight Research Center (predecessor of the Dryden Flight Research Center, Edwards, CA) in a section of the calibration hangar dubbed 'Wright Bicycle Shop.' Visible are the stick, rudder pedals, and ejection seat. The external wooden shell was attached to the steel structure. The wingless, lifting body aircraft design was initially conceived as a means of landing an aircraft horizontally after atmospheric reentry. The absence of wings would make the extreme heat of re-entry less damaging to the vehicle. In 1962, Dryden management approved a program to build a lightweight, unpowered lifting body as a prototype to flight test the wingless concept. It would look like a 'flying bathtub,' and was designated the M2-F1, the 'M' referring to 'manned' and 'F' referring to 'flight' version. It featured a plywood shell placed over a tubular steel frame crafted at Dryden. Construction was completed in 1963. The first flight tests of the M2-F1 were over Rogers Dry Lake at the end of a tow rope attached to a hopped-up Pontiac convertible driven at speeds up to about 120 mph. This vehicle needed to be able to tow the M2-F1 on the Rogers Dry Lakebed adjacent to NASA's Flight Research Center (FRC) at a minimum speed of 100 miles per hour. To do that, it had to handle the 400-pound pull of the M2-F1. Walter 'Whitey' Whiteside, who was a retired Air Force maintenance officer working in the FRC's Flight Operations Division, was a dirt-bike rider and hot-rodder. Together with Boyden 'Bud' Bearce in the Procurement and Supply Branch of the FRC, Whitey acquired a Pontiac Catalina convertible with the largest engine available. He took the car to Bill Straup's renowned hot-rod shop near Long Beach for modification. With a special gearbox and racing slicks, the Pontiac could tow the 1,000-pound M2-F1 110 miles per hour in 30 seconds. It proved adequate for the roughly 400 car tows that got the M2-F1 airborne to prove it could fly

  10. Dressed rates and muscular nutrients in northern snakehead Channa argus,Taiwan snakehead C.maculata and their hybrids%乌鳢、 斑鳢及其杂交F1 代肌肉营养成分和含肉率的比较分析

    Institute of Scientific and Technical Information of China (English)

    罗青; 陈昆慈; 赵建; 洪效友; 朱新平; 史燕

    2015-01-01

    为了进一步研究杂交鳢的经济性状和杂交优势, 采用常规生化分析方法, 测定乌鳢Channa argus、斑鳢C.maculata、 乌斑鳢C.argus♀×C.maculata♂和斑乌鳢C.maculata♀×C.argus♂的含肉率及肌肉营养成分, 对其营养价值进行比较分析, 并评价了其肌肉品质.结果表明: 乌鳢、 斑鳢、 乌斑鳢、 斑乌鳢平均含肉率分别为 68.24%、 71.34%、 70.71%、 70.18%; 乌鳢、 斑鳢、 乌斑鳢和斑乌鳢粗蛋白质含量为20.6% ~ 21.4%, 粗灰分含量为1.2% ~1.5%, 水分含量为76.1% ~76.7%, 脂肪含量<1.7%; 必需氨基酸含量占氨基酸总量的49.40% ~49.65%, 鲜味氨基酸含量占氨基酸总量的38.35% ~38.87%, 其中乌斑鳢的必需氨基酸指数(EAAI)和鲜味氨基酸含量均高于乌鳢、 斑鳢和斑乌鳢.研究表明, 乌鳢、 斑鳢、乌斑鳢和斑乌鳢肌肉中蛋白质含量均较高, 脂肪含量较低, 氨基酸种类齐全, 必需氨基酸与鲜味氨基酸含量较高, 乌斑鳢蛋白质营养水平与乌鳢、 斑鳢和斑乌鳢相当, 但其蛋白质氨基酸水平所体现的肌肉品质则优于乌鳢、 斑鳢和斑乌鳢.%Dressed rates and muscular nutrients were analyzed and evaluated in northern snakehead Channa argus, Taiwan snakehead C.maculata and their hybrids(C.argus♀×C.maculata♂, and C.maculata ♀×C.argus ♂).The dressed rate was shown to be 68.24% in northern snakehead, 70.18% in C.maculata♀×C.argus♂, 71.34% in Taiwan snakehead, and 70.71% in C.argus♀×C.maculata♂.Northern snakehead and C.maculata♀×C.argus♂ had crude protein of 20.6%-21.4%, moisture of 76.1%-76.7%(less fluctuation), crude fat content<1.7%(more fluctuation), and ash of 1.2%-1.5% in muscle.The C.maculata♀×C.argus ♂ had crude fat content of 1.6% and the hybrid C.argus♀×C.maculata♂1.7%, higher than northern snakehead and Taiwan snakehead did.Sixteen common amino acids, and nine essential amino acids for human needs, accounting for 49.40%-49.65% of

  11. Urbanization and the Level of Microplastic Ingestion by Fish: A Comparison of Freshwater Sunfish (Centrarchidae) from the Brazos River watershed, and Pinfish (Sparidae), from the Brazos Estuary and Inshore Marine Sites, Texas, USA

    Science.gov (United States)

    Rieper, K. B.; Peters, C. A.; Bratton, S. P.

    2016-02-01

    While previous research has documented ingestion of macro- and microplastics by aquatic fauna in both freshwater and marine ecosystems, relatively little is known of the environmental and ecological factors influencing the entry and diffusion of plastics and artificial polymers into aquatic foodwebs. Microplastics are defined as 50 μm to 5 mm in length. This study utilized stomach content analysis to compare the level of microplastic artificial polymer ingestion for fish collected from the Brazos River watershed, Brazos estuary, and inshore coastal waters of Texas, USA, in areas with varying levels of urbanization. We collected 318 bluegill (Lepomis macrochirus) and 118 longear sunfish (Lepomis megalotis) at 14 freshwater locales, and 11 samples of 298 pinfish (Lagodon rhomboides) at 6 saltwater locales. Sunfish averaged 12.6 cm in length, and pinfish averaged 14.9 cm. Sunfish averaged .807 microplastics per fish, and pinfish averaged 1.09. The maximum percentage for pinfish with microplastics present per sample (frequency) was 77%, compared to 75% for sunfish. Mean frequencies per sample were also similar: 45% for sunfish and 47% for pinfish. The Brazos River collections, however, had a greater percentage with frequencies of microplastics (cc=.742) and the mean number of plastic particles ingested per fish (cc=.697). The majority of the microplastics were thread shaped, with blue and grey the dominant colors. Comparison with presence of natural food items suggests microplastic ingestion is predominantly incidental for these sentinel fish species.

  12. Phylogeography and conservation genetics of a distinct lineage of sunfish in the Cuatro Ciénegas valley of Mexico.

    Science.gov (United States)

    Coghill, Lyndon M; Hulsey, C Darrin; Chaves-Campos, Johel; García de Leon, Francisco J; Johnson, Steven G

    2013-01-01

    The valley of Cuatro Ciénegas, an aquatic oasis located in the Mexican Chihuahuan Desert, exhibits the highest level of endemism in North America and is a Mexican National Protected Area. However, little is known about the evolutionary distinctiveness of several vertebrate species present in the Cuatro Ciénegas valley. We conducted a phylogeographic study using mitochondrial haplotypes from the centrarchid fish Lepomis megalotis to determine if the populations found within the valley were evolutionarily distinct from populations outside the valley. We also examined if there was evidence of unique haplotypes of this sunfish within the valley. Genetic divergence of L. megalotis suggests populations within the valley are evolutionarily unique when compared to L. megalotis outside the valley. Significant mitochondrial sequence divergence was also discovered between L. megalotis populations on either side of the Sierra de San Marcos that bisects the valley. Our results reinforce previous studies that suggest the organisms occupying aquatic habitats not only within Cuatro Ciénegas but also in each of the two lobes of the valley generally deserve independent consideration during management decisions.

  13. Effects of some components of acid-mine drainage and acid deposition on the spermatozoa of longear sunfish, Lepomis megalotis

    Energy Technology Data Exchange (ETDEWEB)

    Pearson, B.J.

    1983-01-01

    The effects of low pH and the metals aluminum, zinc, and cadmium, components of acid-mine effluents and acid deposition, on spermatozoa of longear sunfish, Lepomis megalotis, were investigated. Sperm were exposed to solutions of 400 ppm aluminum chloride, 50 ppm zinc chloride, 2 ppm cadmium chloride, separately and in combination, at pH values of 6.9, 4.8, and 3.8. Sperm were additionally exposed to test solutions in which the metal salt concentration was reduced by one-half and observed for changes in motility and in the ability to exclude stain. All test solutions at a low pH were deleterious, the greatest damage occurring in solutions of a combination of all 3 metal chlorides and of aluminum chloride separately. Motility tests showed that both full and reduced metal concentrations had significant effects on motility. Staining tests were supportive of motility test results and indicated that in most cases shorter exposure times did not significantly improve survival rates. It was generally found that a decrease in pH increased the effects of each metal separately and when combined. Aluminum, zinc, and cadmium chlorides appeared to act antagonistically when tested in combination. It was concluded that the components of acid waters which were tested have deleterious effects on longer spermatozoa, reducing their viability and thereby reducing reproductive success of the species.

  14. Morphological change and phenotypic plasticity in native and non-native pumpkinseed sunfish in response to sustained water velocities.

    Science.gov (United States)

    Yavno, S; Fox, M G

    2013-11-01

    Phenotypic plasticity can contribute to the proliferation and invasion success of nonindigenous species by promoting phenotypic changes that increase fitness, facilitate range expansion and improve survival. In this study, differences in phenotypic plasticity were investigated using young-of-year pumpkinseed sunfish from colonies established with lentic and lotic populations originating in Canada (native) and Spain (non-native). Individuals were subjected to static and flowing water treatments for 80 days. Inter- and intra-population differences were tested using ancova and discriminant function analysis, and differences in phenotypic plasticity were tested through a manova of discriminant function scores. Differences between Iberian and North American populations were observed in dorsal fin length, pectoral fin position and caudal peduncle length. Phenotypic plasticity had less influence on morphology than genetic factors, regardless of population origin. Contrary to predictions, Iberian pumpkinseed exhibited lower levels of phenotypic plasticity than native populations, suggesting that canalization may have occurred in the non-native populations during the processes of introduction and range expansion.

  15. Phylogeography and conservation genetics of a distinct lineage of sunfish in the Cuatro Cienegas valley of Mexico.

    Directory of Open Access Journals (Sweden)

    Lyndon M Coghill

    Full Text Available The valley of Cuatro Ciénegas, an aquatic oasis located in the Mexican Chihuahuan Desert, exhibits the highest level of endemism in North America and is a Mexican National Protected Area. However, little is known about the evolutionary distinctiveness of several vertebrate species present in the Cuatro Ciénegas valley. We conducted a phylogeographic study using mitochondrial haplotypes from the centrarchid fish Lepomis megalotis to determine if the populations found within the valley were evolutionarily distinct from populations outside the valley. We also examined if there was evidence of unique haplotypes of this sunfish within the valley. Genetic divergence of L. megalotis suggests populations within the valley are evolutionarily unique when compared to L. megalotis outside the valley. Significant mitochondrial sequence divergence was also discovered between L. megalotis populations on either side of the Sierra de San Marcos that bisects the valley. Our results reinforce previous studies that suggest the organisms occupying aquatic habitats not only within Cuatro Ciénegas but also in each of the two lobes of the valley generally deserve independent consideration during management decisions.

  16. The Appell function F1 and Regge string scattering amplitudes

    Directory of Open Access Journals (Sweden)

    Jen-Chi Lee

    2014-12-01

    Full Text Available We show that each 26D open bosonic Regge string scattering amplitude (RSSA can be expressed in terms of one single Appell function F1 in the Regge limit. This result enables us to derive infinite number of recurrence relations among RSSA at arbitrary mass levels, which are conjectured to be related to the known SL(5,C dynamical symmetry of F1. In addition, we show that these recurrence relations in the Regge limit can be systematically solved so that all RSSA can be expressed in terms of one amplitude. All these results are dual to high energy symmetries of fixed angle string scattering amplitudes discovered previously [4–8].

  17. Regulation of the thermoalkaliphilic F1-ATPase from Caldalkalibacillus thermarum

    Science.gov (United States)

    Ferguson, Scott A.; Cook, Gregory M.; Montgomery, Martin G.; Leslie, Andrew G. W.

    2016-01-01

    The crystal structure has been determined of the F1-catalytic domain of the F-ATPase from Caldalkalibacillus thermarum, which hydrolyzes adenosine triphosphate (ATP) poorly. It is very similar to those of active mitochondrial and bacterial F1-ATPases. In the F-ATPase from Geobacillus stearothermophilus, conformational changes in the ε-subunit are influenced by intracellular ATP concentration and membrane potential. When ATP is plentiful, the ε-subunit assumes a “down” state, with an ATP molecule bound to its two C-terminal α-helices; when ATP is scarce, the α-helices are proposed to inhibit ATP hydrolysis by assuming an “up” state, where the α-helices, devoid of ATP, enter the α3β3-catalytic region. However, in the Escherichia coli enzyme, there is no evidence that such ATP binding to the ε-subunit is mechanistically important for modulating the enzyme’s hydrolytic activity. In the structure of the F1-ATPase from C. thermarum, ATP and a magnesium ion are bound to the α-helices in the down state. In a form with a mutated ε-subunit unable to bind ATP, the enzyme remains inactive and the ε-subunit is down. Therefore, neither the γ-subunit nor the regulatory ATP bound to the ε-subunit is involved in the inhibitory mechanism of this particular enzyme. The structure of the α3β3-catalytic domain is likewise closely similar to those of active F1-ATPases. However, although the βE-catalytic site is in the usual “open” conformation, it is occupied by the unique combination of an ADP molecule with no magnesium ion and a phosphate ion. These bound hydrolytic products are likely to be the basis of inhibition of ATP hydrolysis. PMID:27621435

  18. Rotation and structure of FoF1-ATP synthase.

    Science.gov (United States)

    Okuno, Daichi; Iino, Ryota; Noji, Hiroyuki

    2011-06-01

    F(o)F(1)-ATP synthase is one of the most ubiquitous enzymes; it is found widely in the biological world, including the plasma membrane of bacteria, inner membrane of mitochondria and thylakoid membrane of chloroplasts. However, this enzyme has a unique mechanism of action: it is composed of two mechanical rotary motors, each driven by ATP hydrolysis or proton flux down the membrane potential of protons. The two molecular motors interconvert the chemical energy of ATP hydrolysis and proton electrochemical potential via the mechanical rotation of the rotary shaft. This unique energy transmission mechanism is not found in other biological systems. Although there are other similar man-made systems like hydroelectric generators, F(o)F(1)-ATP synthase operates on the nanometre scale and works with extremely high efficiency. Therefore, this enzyme has attracted significant attention in a wide variety of fields from bioenergetics and biophysics to chemistry, physics and nanoscience. This review summarizes the latest findings about the two motors of F(o)F(1)-ATP synthase as well as a brief historical background.

  19. Identification of transcriptome SNPs between Xiphophorus lines and species for assessing allele specific gene expression within F1 interspecies hybrids☆

    Science.gov (United States)

    Shen, Yingjia; Catchen, Julian; Garcia, Tzintzuni; Amores, Angel; Beldroth, Ion; Wagner, Jonathon R; Zhang, Ziping; Postlethwait, John; Warren, Wes; Schartl, Manfred; Walter, Ronald B.

    2011-01-01

    Variations in gene expression are essential for the evolution of novel phenotypes and for speciation. Studying allelic specific gene expression (ASGE) within interspecies hybrids provides a unique opportunity to reveal underlying mechanisms of genetic variation. Using Xiphophorus interspecies hybrid fishes and high-throughput next generation sequencing technology, we were able to assess variations between two closely related vertebrate species, X. maculatus and X. couchianus, and their F1 interspecies hybrids. We constructed transcriptome-wide SNP polymorphism sets between two highly inbred X. maculatus lines (JP 163 A and B), and between X. maculatus and a second species, X. couchianus. The X. maculatus JP 163 A and B parental lines have been separated in the laboratory for ≈ 70 years and we were able to identify SNPs at a resolution of 1 SNP per 49 kb of transcriptome. In contrast, SNP polymorphisms between X. couchianus and X. maculatus species, which diverged ≈ 5–10 million years ago, were identified about every 700 bp. Using 6,524 transcripts with identified SNPs between the two parental species (X. maculatus and X. couchianus), we mapped RNA-seq reads to determine ASGE within F1 interspecies hybrids. We developed an in silico X. couchianus transcriptome by replacing 90,788 SNP bases for X. maculatus transcriptome with the consensus X. couchianus SNP bases and provide evidence that this procedure overcomes read mapping biases. Employment of the insilico reference transcriptome and tolerating 5 mismatches during read mapping allow direct assessment of ASGE in the F1 interspecies hybrids. Overall, these results show that Xiphophorus is a tractable vertebrate experimental model to investigate how genetic variations that occur during speciation may affect gene interactions and the regulation of gene expression. PMID:21466860

  20. f~(-1)[f(x)]=x和f[f~(-1)(x)]=x(高一、高二、高三)

    Institute of Scientific and Technical Information of China (English)

    曾家骏

    2002-01-01

    请看下面两个题目: 1.已知函数f(x)=(3x-2)/(2x+7),则f[f-1(x)]=_______; 2.若g(x)=2x3+4,则g1[g(x)]=_______. 这是一本高中数学辅导书上的两个练习题,原解答是先分别求出反函数f-1(x)和g-1(x)后,再代入复合计算得出结果,答案都是x.其实,这些计算都是多余的,无论f(x)和

  1. Piceatannol, a stilbene phytochemical, inhibits mitochondrial F0F1-ATPase activity by targeting the F1 complex.

    Science.gov (United States)

    Zheng, J; Ramirez, V D

    1999-08-02

    Piceatannol is a stilbene phytochemical from the seeds of Euphorbia lagascae, previously identified as an antileukemic principle. Piceatannol is considered an inhibitor of several tyrosine kinases. We recently reported that resveratrol, another stilbene phytoalexin from grape seeds, was an inhibitor of ATP synthase. Here, we demonstrated that piceatannol potently inhibited the rat brain mitochondrial F0F1-ATPase activity in both solubilized and submitochondrial preparations (IC50 of 8-9 microM), while having relatively small effect on the Na(+), K(+)-ATPase activity of porcine cerebral cortex (no effect up to 7 microM). Piceatannol inhibited the ATPase activity of the purified rat liver F1 with IC50 of about 4 microM, while resveratrol was slightly less active (IC50 of about 14 microM). Our results indicate that piceatannol and resveratrol inhibit the F-type ATPase by targeting the F1 sector, which is located to the inner membrane of mitochondria and plasma membrane of normal endothelial cells and several cancer cell lines. This mechanism could potentially contribute to the multiple effects of these chemopreventive phytochemicals. Copyright 1999 Academic Press.

  2. Non-leptonic decays of $B \\to ( f_1(1285),f_1(1420) ) V$ in the perturbative QCD approach

    CERN Document Server

    Liu, Xin; Zou, Zhi-Tian

    2016-01-01

    We investigate the branching ratios, the polarization fractions, the direct CP-violating asymmetries, and the relative phases in 20 non-leptonic decay modes of $B \\to f_1 V$ within the framework of perturbative QCD approach at leading order with $f_1$ including two $^3\\!P_1$-axial-vector states $f_1(1285)$ and $f_1(1420)$. Here, $B$ denotes $B^+$, $B^0$, and $B_s^0$ mesons and $V$ stands for the lightest vector mesons $\\rho$, $K^*$, $\\omega$, and $\\phi$ , respectively. The $B_s^0 \\to f_1 V$ decays are studied theoretically for the first time in the literature. Together with the angle $\\phi_{f_1} \\approx (24^{+3.2}_{-2.7})^\\circ$ extracted from the measurement through $B_{d/s} \\to J/\\psi f_1(1285)$ modes for the $f_1(1285)-f_1(1420)$ mixing system, it is of great interest to find phenomenologically that some modes such as the tree-dominated $B^+ \\to f_1 \\rho^+$ and the penguin-dominated $B^{+,0} \\to f_1 K^{*+,0}, B_s^0 \\to f_1 \\phi$ with large branching ratios around ${\\cal O}(10^{-6})$ or even ${\\cal O}(10^{-...

  3. Cleanup Verification Package for the 118-F-1 Burial Ground

    Energy Technology Data Exchange (ETDEWEB)

    E. J. Farris and H. M. Sulloway

    2008-01-10

    This cleanup verification package documents completion of remedial action for the 118-F-1 Burial Ground on the Hanford Site. This burial ground is a combination of two locations formerly called Minor Construction Burial Ground No. 2 and Solid Waste Burial Ground No. 2. This waste site received radioactive equipment and other miscellaneous waste from 105-F Reactor operations, including dummy elements and irradiated process tubing; gun barrel tips, steel sleeves, and metal chips removed from the reactor; filter boxes containing reactor graphite chips; and miscellaneous construction solid waste.

  4. On fundamental groups related to the Hirzebruch surface F1

    Institute of Scientific and Technical Information of China (English)

    Michael; FRIEDMAN; Mina; TEICHER

    2008-01-01

    Given a projective surface and a generic projection to the plane,the braid monodromy factorization(and thus,the braid monodromy type)of the complement of its branch curve is one of the most important topological invariants,stable on deformations.From this factorization,one can compute the fundamental group of the complement of the branch curve,either in C2 or in CP2.In this article,we show that these groups,for the Hirzebruch surface F1,(a,b),are almost-solvable.That is, they are an extension of a solvable group,which strengthen the conjecture on degeneratable surfaces.

  5. On fundamental groups related to the Hirzebruch surface F1

    Institute of Scientific and Technical Information of China (English)

    Michael FRIEDMAN; Mina TEICHER

    2008-01-01

    Given a.projective surface and a generic projection to the plane,the braid monodromy factorization (and thus,the braid monodromy type) of the complement of its branch curve is one of the most important topological invariants,stable on deformations.From this factorization,one can compute the fundamental group of the complement of the branch curve,either in C2 or in CP2.In this article,we show that these groups,for the Hirzebruch surface F1,(a,b),are almost-solvable.That is,they are an extension of a solvable group,which strengthen the conjecture on degeneratable surfaces.

  6. E2F-1 as an anticancer drug target

    Directory of Open Access Journals (Sweden)

    Joseph R. Bertino

    2011-12-01

    Full Text Available Mounting evidence indicates that the E2F transcription factors play an essential role in all aspects of cellular functions. Many human malignancies have been shown to overexpress one or more of the ‘‘activating’’ E2Fs. In some circumstances, down regulation as well as overexpression of E2F-1, leads to inhibition of cell growth. The emphasis in this review is placed on new data implicating microRNAs in the regulation of E2F activity and the efforts thus far to target this activity in order to cause tumor regression.

  7. Data of evolutionary structure change: 1COBB-1F1GD [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1COBB-1F1GD 1COB 1F1G B D ATKAVCVLKGDGPVQGTIHFEAKG--DTVVVTGSITGLTE-GDHGFHVHQFGD...NTQGCTSAGPHFNPLSKKHGGPKDEERHVGDLGNVTADKNGVAIVDIVDPLISLSGEYSIIGRTMVVHEKPDDLGRGGNEESTKTGNAGSRLAC...GVIGIAK -VQAVAVLKGDAGVSGVVKFEQASESEPTTVSYEIAGNSPNAERGFHIHEFGDATNGCVSAGPHFNPFKKTHGAPTDEVRHVGDMGN...VKTDENGVAKGSFKDSLIKLIGPTSVVGRSVVIHAGQDDLGKGDTEESLKTGNAGPRPACGVIGLTN ...pdbID>1F1G D 1F1GD FEQASESEPTTV

  8. Trace element content of fish feed and bluegill sunfish (Lepomis macrochirus) from aquaculture and wild source in Missouri.

    Science.gov (United States)

    Ikem, Abua; Egilla, Jonathan

    2008-09-15

    Trace element content of fish feed and bluegill sunfish muscles (Lepomis macrochirus) from aquaculture and natural pond in Missouri were determined using the inductively coupled-plasma optical emission spectrometer (ICP-OES) and the direct mercury analyzer (DMA). Dietary intake rates of trace elements were estimated. Dogfish muscle (DORM-2) and lobster hepatopancreas (TORT-2) reference standards were used in trace element recovery and method validations. The average elemental concentrations (mg/kg diet, dry wt.) of fish feed were: As 1.81, Cd 2.37, Co 0.10, Cr 1.42, Cu 8.0, Fe 404, Mn 35.9, Ni 0.51, Pb 9.16, Se 1.71, Sn 20.7, V 0.09, Zn 118 and Hg 0.07. The mean elemental concentrations (μg/kg wet wt.) in bluegill muscles from both aquaculture and wild (in parenthesis) sources were: As 0.36 (0.06), Cd 0.28 (0.01), Co 0.0 (0.0), Cr 0.52 (0.05), Cu 0.38 (0.18), Fe 17.5 (2.43), Mn 0.18 (0.24), Ni 0.18 (0.04), Pb 1.03 (0.04), Se 0.34 (0.30), Sn 0.66 (0.42), V 0.02 (0.01), Zn 6.97 (9.13) and Hg 0.06 (0.24). Kruskal-Wallis chi square indicated significant differences in As, Cd, Co, Cr, Cu, Fe, Ni, Pb, Sn, V, Zn and Hg (Paquaculture and wild bluegills, posed no health risks for approximately 85% of all samples.

  9. Photoproduction of the $f_1(1285)$ Meson

    CERN Document Server

    Dickson, R

    2016-01-01

    The $f_1(1285)$ meson with mass $1281.0 \\pm 0.8$ MeV/$c^2$ and width $18.4 \\pm 1.4$ MeV (FWHM) was measured for the first time in photoproduction from a proton target using CLAS at Jefferson Lab. Differential cross sections were obtained via the $\\eta\\pi^{+}\\pi^{-}$, $K^+\\bar{K}^0\\pi^-$, and $K^-K^0\\pi^+$ decay channels from threshold up to a center-of-mass energy of 2.8 GeV. The mass, width, and an amplitude analysis of the $\\eta\\pi^{+}\\pi^{-}$ final-state Dalitz distribution are consistent with the axial-vector $J^P=1^+$ $f_1(1285)$ identity, rather than the pseudoscalar $0^-$ $\\eta(1295)$. The production mechanism is more consistent with $s$-channel decay of a high-mass $N^*$ state, and not with $t$-channel meson exchange. Decays to $\\eta\\pi\\pi$ go dominantly via the intermediate $a_0^\\pm(980)\\pi^\\mp$ states, with the branching ratio $\\Gamma(a_0\\pi \\text{ (no} \\bar{K} K\\text{)}) / \\Gamma(\\eta\\pi\\pi \\text{(all)}) = 0.74\\pm0.09$. The branching ratios $\\Gamma(K \\bar{K} \\pi)/\\Gamma(\\eta\\pi\\pi) = 0.216\\pm0.033$...

  10. cDNA library Table: F1mg [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available F1mg NA F1mg F1 (J150 x J203) midgut fourth instar larval stage D2 mixed pBluescrip...t SK- EcoR1 for 5' Xho1for 3' sequenced from T3 primer (5' -> 3') BY917461-BY918785,BY925786-BY927071 E_ET_F1mg_[number]_F_0,E_ET_F1mg_[number]_R_0 ...

  11. E2F-1 binding affinity for pRb is not the only determinant of the E2F-1 activity.

    Science.gov (United States)

    Sahin, Fikret; Sladek, Todd L

    2010-07-04

    E2F-1 is the major cellular target of pRB and is regulated by pRB during cell proliferation. Interaction between pRB and E2F-1 is dependent on the phosphorylation status of pRB. Despite the fact that E2F-1 and pRB have antagonistic activities when they are overexpressed, the role of the E2F-1-pRB interaction in cell growth largely remains unknown. Ideally, it would be better to study the properties of a pRB mutant that fails to bind to E2F, but retains all other activities. To date, no pRB mutation has been characterized in sufficient detail to show that it specifically eliminates E2F binding but leaves other interactions intact. An alternative approach to this issue is to ask whether mutations that change E2F proteins binding affinity to pRB are sufficient to change cell growth in aspect of cell cycle and tumor formation. Therefore, we used the E2F-1 mutants including E2F-1/S332-7A, E2F-1/S375A, E2F-1/S403A, E2F-1/Y411A and E2F-1/L132Q that have different binding affinities for pRB to better understand the roles of the E2F-1 phosphorylation and E2F-1-pRB interaction in the cell cycle, as well as in transformation and gene expression. Data presented in this study suggests that in vivo phosphorylation at amino acids 332-337, 375 and 403 is important for the E2F-1 and pRB interaction in vivo. However, although E2F-1 mutants 332-7, 375 and 403 showed similar binding affinity to pRB, they showed different characteristics in transformation efficiency, G(0) accumulation, and target gene experiments.

  12. Measurement of Inclusive $f_1$(1285) and $f_1$(1420) Production in Z Decays with the DELPHI Detector

    CERN Document Server

    Abdallah, J; Adam, W; Adzic, P; Albrecht, T; Alderweireld, T; Alemany-Fernandez, R; Allmendinger, T; Allport, P P; Amaldi, Ugo; Amapane, N; Amato, S; Anashkin, E; Andreazza, A; Andringa, S; Anjos, N; Antilogus, P; Apel, W D; Arnoud, Y; Ask, S; Åsman, B; Augustin, J E; Augustinus, A; Baillon, Paul; Ballestrero, A; Bambade, P; Barbier, R; Bardin, Dimitri Yuri; Barker, G; Baroncelli, A; Battaglia, Marco; Baubillier, M; Becks, K H; Begalli, M; Behrmann, A; Ben-Haim, E; Benekos, N C; Benvenuti, Alberto C; Bérat, C; Berggren, M; Berntzon, L; Bertrand, D; Besançon, M; Besson, N; Bloch, D; Blom, M; Bluj, M; Bonesini, M; Boonekamp, M; Booth, P S L; Borisov, G; Botner, O; Bouquet, B; Bowcock, T J V; Boyko, I; Bracko, M; Brenner, R; Brodet, E; Brückman, P; Brunet, J M; Bugge, L; Buschmann, P; Calvi, M; Camporesi, T; Canale, V; Carena, F; Castro, N; Cavallo, F R; Chapkin, M M; Charpentier, P; Checchia, P; Chierici, R; Shlyapnikov, P; Chudoba, J; Chung, S U; Cieslik, K; Collins, P; Contri, R; Cosme, G; Cossutti, F; Costa, M J; Crawley, B; Crennell, D J; Cuevas-Maestro, J; D'Hondt, J; Dalmau, J; Da Silva, T; Da Silva, W; Della Ricca, G; De Angelis, A; de Boer, Wim; De Clercq, C; De Lotto, B; De Maria, N; De Min, A; De Paula, L S; Di Ciaccio, Lucia; Di Simone, A; Doroba, K; Drees, J; Dris, M; Eigen, G; Ekelöf, T J C; Ellert, M; Elsing, M; Espirito-Santo, M C; Fanourakis, G K; Fassouliotis, D; Feindt, M; Fernández, J; Ferrer, A; Ferro, F; Flagmeyer, U; Föth, H; Fokitis, E; Fulda-Quenzer, F; Fuster, J A; Gandelman, M; García, C; Gavillet, P; Gazis, E N; Gokieli, R; Golob, B; Gómez-Ceballos, G; Gonçalves, P; Graziani, E; Grosdidier, G; Grzelak, K; Guy, J; Haag, C; Hallgren, A; Hamacher, K; Hamilton, K; Hansen, J; Haug, S; Hauler, F; Hedberg, V; Hennecke, M; Herr, H; Hoffman, J; Holmgren, S O; Holt, P J; Houlden, M A; Hultqvist, K; Jackson, J N; Jarlskog, G; Jarry, P; Jeans, D; Johansson, E K; Johansson, P D; Jonsson, P; Joram, C; Jungermann, L; Kapusta, F; Katsanevas, S; Katsoufis, E C; Kernel, G; Kersevan, Borut P; Kiiskinen, A P; King, B T; Kjaer, N J; Kluit, P; Kokkinias, P; Kourkoumelis, C; Kuznetsov, O; Krumshtein, Z; Kucharczyk, M; Lamsa, J; Leder, G; Ledroit, F; Leinonen, L; Leitner, R; Lemonne, J; Lepeltier, V; Lesiak, T; Liebig, W; Liko, D; Lipniacka, A; Lopes, J H; López, J M; Loukas, D; Lutz, P; Lyons, L; MacNaughton, J; Malek, A; Maltezos, S; Mandl, F; Marco, J; Marco, R; Maréchal, B; Margoni, M; Marin, J C; Mariotti, C; Markou, A; Martínez-Rivero, C; Masik, J; Mastroyiannopoulos, N; Matorras, F; Matteuzzi, C; Mazzucato, F; Mazzucato, M; McNulty, R; Meroni, C; Meyer, W T; Migliore, E; Mitaroff, W A; Mjörnmark, U; Moa, T; Moch, M; Mönig, K; Monge, R; Montenegro, J; Moraes, D; Moreno, S; Morettini, P; Müller, U; Münich, K; Mulders, M; Mundim, L M; Murray, W; Muryn, B; Myatt, Gerald; Myklebust, T; Nassiakou, M; Navarria, Francesco Luigi; Nawrocki, K; Nicolaidou, R; Nikolenko, M; Oblakowska-Mucha, A; Obraztsov, V F; Olshevskii, A G; Onofre, A; Orava, Risto; Österberg, K; Ouraou, A; Oyanguren, A; Paganoni, M; Paiano, S; Palacios, J P; Palka, H; Papadopoulou, T D; Pape, L; Parkes, C; Parodi, F; Parzefall, U; Passeri, A; Passon, O; Peralta, L; Perepelitsa, V F; Perrotta, A; Petrolini, A; Piedra, J; Pieri, L; Pierre, F; Pimenta, M; Piotto, E; Podobnik, T; Poireau, V; Pol, M E; Polok, G; Poropat, P; Pozdnyakov, V; Pukhaeva, N; Pullia, Antonio; Rames, J; Ramler, L; Read, A; Rebecchi, P; Rehn, J; Reid, D; Reinhardt, R; Renton, P B; Richard, F; Rídky, J; Rivero, M; Rodríguez, D; Romero, A; Ronchese, P; Rosenberg, E I; Roudeau, Patrick; Rovelli, T; Ruhlmann-Kleider, V; Ryabtchikov, D; Sadovskii, A; Salmi, L; Salt, J; Savoy-Navarro, A; Schwickerath, U; Segar, A; Sekulin, R L; Siebel, M; Sissakian, A N; Smadja, G; Smirnova, O G; Sokolov, A; Sopczak, A; Sosnowski, R; Spassoff, Tz; Stanitzki, M; Stocchi, A; Strauss, J; Stugu, B; Szczekowski, M; Szeptycka, M; Szumlak, T; Tabarelli de Fatis, T; Taffard, A C; Tegenfeldt, F; Timmermans, J; Tkatchev, L G; Tobin, M; Todorovova, S; Tomé, B; Tonazzo, A; Tortosa, P; Travnicek, P; Treille, D; Tristram, G; Trochimczuk, M; Troncon, C; Turluer, M L; Tyapkin, I A; Tyapkin, P; Tzamarias, S; Uvarov, V; Valenti, G; van Dam, P; Van Eldik, J; Van Lysebetten, A; Van Remortel, N; Van Vulpen, I B; Vegni, G; Veloso, F; Venus, W A; Verbeure, F; Verdier, P; Verzi, V; Vilanova, D; Vitale, L; Vrba, V; Wahlen, H; Washbrook, A J; Weiser, C; Wicke, D; Wickens, J H; Wilkinson, G; Winter, M; Witek, M; Yushchenko, O P; Zalewska-Bak, A; Zalewski, Piotr; Zavrtanik, D; Zhuravlov, V; Zimin, N I; Zinchenko, A I; Zupan, M

    2003-01-01

    DELPHI results are presented on the inclusive production of two (K Kbar pi)^0 states in the mass region 1.2-1.6 GeV/c^2 in hadronic Z decays at LEP I. The measured masses (widths) are 1274 +/- 6 MeV/c^2 (29 +/- 12 MeV/c^2) and 1426 +/- 6 MeV/c^2 (51 +/- 14 MeV/c^2) respectively. A partial-wave analysis of the (K Kbar pi)^0 system shows that the first peak is consistent with the I^G(J^{PC})=0^+(1^{++})/(0^{-+}) a_0(980)pi and the second with the I^G(J^{PC})=0^+(1^{++}) K^*(892)Kbar + c.c. assignments. The total hadronic production rates per hadronic Z decay are (0.165 +/- 0.051) and (0.056 +/- 0.012) respectively. These measurements are consistent with the two states being the f_1(1285) and f_1(1420) mesons.

  13. Contemporary and historical evolutionary processes interact to shape patterns of within-lake phenotypic divergences in polyphenic pumpkinseed sunfish, Lepomis gibbosus.

    Science.gov (United States)

    Weese, Dylan J; Ferguson, Moira M; Robinson, Beren W

    2012-03-01

    Historical and contemporary evolutionary processes can both contribute to patterns of phenotypic variation among populations of a species. Recent studies are revealing how interactions between historical and contemporary processes better explain observed patterns of phenotypic divergence than either process alone. Here, we investigate the roles of evolutionary history and adaptation to current environmental conditions in structuring phenotypic variation among polyphenic populations of sunfish inhabiting 12 postglacial lakes in eastern North America. The pumpkinseed sunfish polyphenism includes sympatric ecomorphs specialized for littoral or pelagic lake habitats. First, we use population genetic methods to test the evolutionary independence of within-lake phenotypic divergences of ecomorphs and to describe patterns of genetic structure among lake populations that clustered into three geographical groupings. We then used multivariate analysis of covariance (MANCOVA) to partition body shape variation (quantified with geometric morphometrics) among the effects of evolutionary history (reflecting phenotypic variation among genetic clusters), the shared phenotypic response of all populations to alternate habitats within lakes (reflecting adaptation to contemporary conditions), and unique phenotypic responses to habitats within lakes nested within genetic clusters. All effects had a significant influence on body form, but the effects of history and the interaction between history and contemporary habitat were larger than contemporary processes in structuring phenotypic variation. This highlights how divergence can be better understood against a known backdrop of evolutionary history.

  14. Single molecule energetics of F1-ATPase motor.

    Science.gov (United States)

    Muneyuki, Eiro; Watanabe-Nakayama, Takahiro; Suzuki, Tetsuya; Yoshida, Masasuke; Nishizaka, Takayuki; Noji, Hiroyuki

    2007-03-01

    Motor proteins are essential in life processes because they convert the free energy of ATP hydrolysis to mechanical work. However, the fundamental question on how they work when different amounts of free energy are released after ATP hydrolysis remains unanswered. To answer this question, it is essential to clarify how the stepping motion of a motor protein reflects the concentrations of ATP, ADP, and P(i) in its individual actions at a single molecule level. The F(1) portion of ATP synthase, also called F(1)-ATPase, is a rotary molecular motor in which the central gamma-subunit rotates against the alpha(3)beta(3) cylinder. The motor exhibits clear step motion at low ATP concentrations. The rotary action of this motor is processive and generates a high torque. These features are ideal for exploring the relationship between free energy input and mechanical work output, but there is a serious problem in that this motor is severely inhibited by ADP. In this study, we overcame this problem of ADP inhibition by introducing several mutations while retaining high enzymatic activity. Using a probe of attached beads, stepping rotation against viscous load was examined at a wide range of free energy values by changing the ADP concentration. The results showed that the apparent work of each individual step motion was not affected by the free energy of ATP hydrolysis, but the frequency of each individual step motion depended on the free energy. This is the first study that examined the stepping motion of a molecular motor at a single molecule level with simultaneous systematic control of DeltaG(ATP). The results imply that microscopically defined work at a single molecule level cannot be directly compared with macroscopically defined free energy input.

  15. Embryogenesis from isolated microspores of tulip : towards developing F1 hybrid varieties

    NARCIS (Netherlands)

    Custers, J.B.M.; Ennik, E.; Eikelboom, W.; Dons, J.J.M.; Lookeren Campagne, van M.M.

    1997-01-01

    This report describes advances in the technique of embryogenesis from tulip microspores in culture. High temperature pretreatment (32°C) of bulbs, which contain fully developed inflorescences, had a positive effect on the production of microspore embryos. The pretreatment shortened the time for

  16. Backcrosses in interspecific hybridization in sunflower

    Directory of Open Access Journals (Sweden)

    Atlagić Jovanka

    2003-01-01

    Full Text Available When incorporating desirable traits (resistance to causal agents of various diseases from the wild relatives into the cultivated sunflower, some undesirable ones are introduced too (branching, small head diameter, low oil content, etc. To overcome this problem, backcrosses (F1 interspecific hybrids x cultivated sunflower are used, although very often desirable traits are lost in the process. Cytological analysis (meiosis and pollen viability and molecular markers (RAPD were used to estimate what portion of the parental species genome was present in (be interspecific hybrids of the F1 and BC1F1 generations. The results showed that the percentage of irregularities at meiosis increased from F1 to BC1F1 gen. They also indicated the presence of aneuploids and sterility in the cross between the hexaploid species H.rigidus and cultivated sunflower. The genetic distance between the parents was 83%, that between H.rigidus and the F1 hybrid 54 61%, and that between H.annuus and F1 hybrid 70-76%. In the BC1F1 generation, the genetic distance from Hannuus decreased to 58-66% and that from H.rigidus increased to 69-76%.

  17. Photoproduction of Hybrid Mesons

    CERN Document Server

    Barnes, T

    1998-01-01

    In this contribution I discuss prospects for photoproducing hybrid mesons at CEBAF, based on recent model results and experimental indications of possible hybrids. One excellent opportunity appears to be a search for the I=1, JPC=2+-, neutral "(b2)o" hybrid in (a2 pi)o through diffractive photoproduction. Other notable possibilities accessible through pi+ or pio exchange photoproduction are I=1, JPC=1-+, charged "pi1+" in f1 pi+, (b1 pi)+ and (rho pi)+; piJ(1770)+ in f2 pi+ and (b1 pi)+; pi(1800)+ in f0 pi+, f2 pi+, omega rho+ and (rho pi)+; a1 in f1 pi+ and f2 pi+; and omega in (rho pi)o, omega eta and (K1 K)o.

  18. F1t3 RECEPTOR EXPRESSION ON THE SURFACE OF MALIGNANT HEMATOPOIETIC CELLS AND RESPONSES TO F1t3 LIGAND STIMULATION

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective: To investigate the F1t3 receptor expression on the surface of malignant hematopoietic cells, the effect of TNFa and dexamethasone (DXM) on its expression and the responses of those cells to recombinant human F1t3 ligand (rhFL). Methods: Eighteen malignant hematopoietic cell lines were determined for the F1t3 receptor expression by flow cytometric analysis. The effect of rhFL on the proliferation of malignant hematopoietic cells in vitro was measured using MTT assay. Results: The expressions of F1t3 receptor on the surface of Raji, Daudi, HL-60, 8266 and XG-6 cells were detected by flow cytometric analysis. Following incubation with 20 ng/ml TNFa for 24h, the number of F1t3 receptor positive cells decreased in Raji and 8266, increased in HL-60 and XG-6, and no difference in Daudi cells. After incubation with 10-6 mol/L DXM for 24h, the number of F1t3 receptor positive cells decreased in all the 5 F1t3 receptor positive cell lines. rhFL stimulated the proliferation of HL-60 and Raji cells. Conclusion: For most of the malignant hematopoietic cells, there was neither the expression of F1t3 receptor nor the response to rhFL. DXM may be useful to reduce the effect of FL on the proliferation of some F1t3 receptor positive malignant hematopoietic cells in vitro and in vivo.

  19. Age-associated changes in hearts of male Fischer 344/Brown Norway F1 rats.

    Science.gov (United States)

    Walker, Ernest M; Nillas, Michael S; Mangiarua, Elsa I; Cansino, Sylvestre; Morrison, Ryan G; Perdue, Romaine R; Triest, William E; Wright, Gary L; Studeny, Mark; Wehner, Paulette; Rice, Kevin M; Blough, Eric R

    2006-01-01

    Aging is associated with left ventricular hypertrophy, dilatation, and fibrosis of the heart. The Fischer 344/Brown Norway F1 (F344/BNF1) rat is recommended for age-related studies by the National Institutes on Aging because this hybrid rat lives longer and has a lower rate of pathological conditions than inbred rats. However, little is known about age-associated changes in cardiac and aortic function and structure in this model. This study evaluated age-related cardiac changes in male F344/BNF1 rats using ECHO, gross, and microscopic examinations. Rats aged 6-, 30-, and 36-mo were anesthetized and two-dimensional ECHO measurements, two-dimensional guided M-mode, Doppler M-mode, and other recordings from parasternal long- and short-axis views were obtained using a Phillips 5500 ECHO system with a 12 megahertz transducer. Hearts and aortas from sacrificed rats were evaluated grossly and microscopically. The ECHO studies revealed persistent cardiac arrhythmias (chiefly PVCs) in 72% (13/18) of 36-mo rats, 10% (1/10) of 30-mo rats, and none in 6-mo rats (0/16). Gross and microscopic studies showed left ventricular (LV) dilatation, borderline to mild hypertrophy, and areas of fibrosis that were common in 36-mo rats, less evident in 30-mo rats, and absent in 6-mo rats. Aging was associated with mild to moderate decreases of LV diastolic and systolic function. Thus, male F344/BN F1 rats demonstrated progressive age-related (a) decline in cardiac function (diastolic and systolic indices), (b) LV structural changes (chamber dimensions, volumes, and wall thicknesses), and (c) persistent arrhythmias. These changes are consistent with those in humans. The noninvasive ECHO technique offers a means to monitor serial age-related cardiac failure and therapeutic responses in the same rats over designated time intervals.

  20. Mg2+ coordination in catalytic sites of F1-ATPase.

    Science.gov (United States)

    Weber, J; Hammond, S T; Wilke-Mounts, S; Senior, A E

    1998-01-13

    Coordination of the Mg2+ ion in Mg-nucleotide substrates by amino acid residue side chains in the catalytic site of Escherichia coli F1-ATPase was investigated. From the X-ray structure of the mitochondrial enzyme [Abrahams, J. P., Leslie, A. G. W., Lutter, R., and Walker, J. E. (1994) Nature 370, 621-628], it may be inferred that the hydroxyl of betaThr-156 is a direct ligand of Mg2+, whereas the carboxyls of betaGlu-181, betaGlu-185, and betaAsp-242 might contribute via intervening water molecules. Elimination of each respective functional group by site-directed mutagenesis, followed by determination of Mg-nucleotide and uncomplexed nucleotide binding affinities using a tryptophan probe, showed that betaThr-156, betaGlu-185, and betaAsp-242 are all involved in Mg2+ coordination, whereas betaGlu-181 is not. A derived structural model for the octahedral coordination around the Mg2+ ion is presented. The results indicate that the ADP-containing site in the X-ray structure is the catalytic site of highest affinity. Correct Mg2+ coordination is required for catalytic activity at physiological rates. Elimination of any one of the Mg2+-coordinating residues led to complete loss of Mg2+-dependent nucleotide binding cooperativity of the catalytic sites.

  1. The biomechanical properties of F1C pili

    CERN Document Server

    Castelain, Mickaël; Klinth, Jeanna; Lindberg, Stina; Andersson, Magnus; Uhlin, Bernt Eric; Axner, Ove

    2014-01-01

    Uropathogenic Escherichia coli (UPEC) express various kinds of organelles, so-called pili or fimbriae, that mediate adhesion to host tissue in the urinary tract through specific receptor-adhesin interactions. The biomechanical properties of these pili have been considered important for the ability of bacteria to withstand shear forces from rinsing urine flows. Force measuring optical tweezers have been used to characterize individual organelles of F1C type expressed by UPEC bacteria with respect to such properties. Qualitatively, the force-vs.-elongation response was found to be similar to that of other types of helix-like pili expressed by UPEC, i.e. type 1, P, and S, with force-induced elongation in three regions of which one represents the important uncoiling mechanism of the helix-like quaternary structure. Quantitatively, the steady-state uncoiling force was assessed to 26.4(1.4) pN, which is similar to those of other pili (which range from 21 pN for SI to 30 pN for type 1). The corner velocity for dynam...

  2. Close phylogenetic relationship between Angolan and Romanian HIV-1 subtype F1 isolates

    Science.gov (United States)

    Guimarães, Monick L; Vicente, Ana Carolina P; Otsuki, Koko; da Silva, Rosa Ferreira FC; Francisco, Moises; da Silva, Filomena Gomes; Serrano, Ducelina; Morgado, Mariza G; Bello, Gonzalo

    2009-01-01

    Background Here, we investigated the phylogenetic relationships of the HIV-1 subtype F1 circulating in Angola with subtype F1 strains sampled worldwide and reconstructed the evolutionary history of this subtype in Central Africa. Methods Forty-six HIV-1-positive samples were collected in Angola in 2006 and subtyped at the env-gp41 region. Partial env-gp120 and pol-RT sequences and near full-length genomes from those env-gp41 subtype F1 samples were further generated. Phylogenetic analyses of partial and full-length subtype F1 strains isolated worldwide were carried out. The onset date of the subtype F1 epidemic in Central Africa was estimated using a Bayesian Markov chain Monte Carlo approach. Results Nine Angolan samples were classified as subtype F1 based on the analysis of the env-gp41 region. All nine Angolan sequences were also classified as subtype F1 in both env-gp120 and pol-RT genomic regions, and near full-length genome analysis of four of these samples confirmed their classification as "pure" subtype F1. Phylogenetic analyses of subtype F1 strains isolated worldwide revealed that isolates from the Democratic Republic of Congo (DRC) were the earliest branching lineages within the subtype F1 phylogeny. Most strains from Angola segregated in a monophyletic group together with Romanian sequences; whereas South American F1 sequences emerged as an independent cluster. The origin of the subtype F1 epidemic in Central African was estimated at 1958 (1934–1971). Conclusion "Pure" subtype F1 strains are common in Angola and seem to be the result of a single founder event. Subtype F1 sequences from Angola are closely related to those described in Romania, and only distantly related to the subtype F1 lineage circulating in South America. Original diversification of subtype F1 probably occurred within the DRC around the late 1950s. PMID:19386115

  3. Mechanistic Basis for Differential Inhibition of the F1Fo-ATPase by Aurovertin

    OpenAIRE

    2009-01-01

    The mitochondrial F1Fo-ATPase performs the terminal step of oxidative phosphorylation. Small molecules that modulate this enzyme have been invaluable in helping decipher F1Fo-ATPase structure, function, and mechanism. Aurovertin is an antibiotic that binds to the β subunits in the F1 domain and inhibits F1Fo-ATPase-catalyzed ATP synthesis in preference to ATP hydrolysis. Despite extensive study and the existence of crystallographic data, the molecular basis of the differential inhibition and ...

  4. Close phylogenetic relationship between Angolan and Romanian HIV-1 subtype F1 isolates

    Directory of Open Access Journals (Sweden)

    Serrano Ducelina

    2009-04-01

    Full Text Available Abstract Background Here, we investigated the phylogenetic relationships of the HIV-1 subtype F1 circulating in Angola with subtype F1 strains sampled worldwide and reconstructed the evolutionary history of this subtype in Central Africa. Methods Forty-six HIV-1-positive samples were collected in Angola in 2006 and subtyped at the env-gp41 region. Partial env-gp120 and pol-RT sequences and near full-length genomes from those env-gp41 subtype F1 samples were further generated. Phylogenetic analyses of partial and full-length subtype F1 strains isolated worldwide were carried out. The onset date of the subtype F1 epidemic in Central Africa was estimated using a Bayesian Markov chain Monte Carlo approach. Results Nine Angolan samples were classified as subtype F1 based on the analysis of the env-gp41 region. All nine Angolan sequences were also classified as subtype F1 in both env-gp120 and pol-RT genomic regions, and near full-length genome analysis of four of these samples confirmed their classification as "pure" subtype F1. Phylogenetic analyses of subtype F1 strains isolated worldwide revealed that isolates from the Democratic Republic of Congo (DRC were the earliest branching lineages within the subtype F1 phylogeny. Most strains from Angola segregated in a monophyletic group together with Romanian sequences; whereas South American F1 sequences emerged as an independent cluster. The origin of the subtype F1 epidemic in Central African was estimated at 1958 (1934–1971. Conclusion "Pure" subtype F1 strains are common in Angola and seem to be the result of a single founder event. Subtype F1 sequences from Angola are closely related to those described in Romania, and only distantly related to the subtype F1 lineage circulating in South America. Original diversification of subtype F1 probably occurred within the DRC around the late 1950s.

  5. 17 CFR 240.12f-1 - Applications for permission to reinstate unlisted trading privileges.

    Science.gov (United States)

    2010-04-01

    ... reinstate unlisted trading privileges. 240.12f-1 Section 240.12f-1 Commodity and Securities Exchanges... Rules and Regulations Under the Securities Exchange Act of 1934 Unlisted Trading § 240.12f-1 Applications for permission to reinstate unlisted trading privileges. (a) An application to reinstate...

  6. Plant Regeneration of Tagetes patula Hybrid Progeny F1%孔雀草杂交F1代的植株再生

    Institute of Scientific and Technical Information of China (English)

    刘娟旭; 王静; 余义勋

    2009-01-01

    以孔雀草(Tagetes patula)子叶、下胚轴和叶片为外植体,通过器官直接发生途径诱导形成不定芽,探讨植物生长调节剂组合、AgNO3浓度、蔗糖浓度和外植体类型等因素对植株再生的影响,建立了再生体系.结果表明:MS+6-BA 1.0 mg·L-1 +NAA 0.5 mg·L-1 +AgNO3 1.0 mg·L-1+蔗糖40 g·L-1培养基最适合不定芽的分化和增殖,子叶不定芽分化率达90%以上,平均每外植体分化不定芽数达5.3个.不定芽较适生根培养基为1/2MS+IAA 0.2 mg·L-1+NAA 0.1 mg·L-1,生根率达到90%.

  7. E2F-1 binding affinity for pRb is not the only determinant of the E2F-1 activity

    Directory of Open Access Journals (Sweden)

    Fikret Sahin, Todd L. Sladek

    2010-01-01

    Full Text Available E2F-1 is the major cellular target of pRB and is regulated by pRB during cell proliferation. Interaction between pRB and E2F-1 is dependent on the phosphorylation status of pRB. Despite the fact that E2F-1 and pRB have antagonistic activities when they are overexpressed, the role of the E2F-1-pRB interaction in cell growth largely remains unknown. Ideally, it would be better to study the properties of a pRB mutant that fails to bind to E2F, but retains all other activities. To date, no pRB mutation has been characterized in sufficient detail to show that it specifically eliminates E2F binding but leaves other interactions intact. An alternative approach to this issue is to ask whether mutations that change E2F proteins binding affinity to pRB are sufficient to change cell growth in aspect of cell cycle and tumor formation. Therefore, we used the E2F-1 mutants including E2F-1/S332-7A, E2F-1/S375A, E2F-1/S403A, E2F-1/Y411A and E2F-1/L132Q that have different binding affinities for pRB to better understand the roles of the E2F-1 phosphorylation and E2F-1-pRB interaction in the cell cycle, as well as in transformation and gene expression. Data presented in this study suggests that in vivo phosphorylation at amino acids 332-337, 375 and 403 is important for the E2F-1 and pRB interaction in vivo. However, although E2F-1 mutants 332-7, 375 and 403 showed similar binding affinity to pRB, they showed different characteristics in transformation efficiency, G0 accumulation, and target gene experiments.

  8. Modulation of the E2F1-driven cancer cell fate by the DNA damage response machinery and potential novel E2F1 targets in osteosarcomas

    DEFF Research Database (Denmark)

    Liontos, Michalis; Niforou, Katerina; Velimezi, Georgia

    2009-01-01

    Osteosarcoma is the most common primary bone cancer. Mutations of the RB gene represent the most frequent molecular defect in this malignancy. A major consequence of this alteration is that the activity of the key cell cycle regulator E2F1 is unleashed from the inhibitory effects of pRb. Studies...... in animal models and in human cancers have shown that deregulated E2F1 overexpression possesses either "oncogenic" or "oncosuppressor" properties, depending on the cellular context. To address this issue in osteosarcomas, we examined the status of E2F1 relative to cell proliferation and apoptosis...... in a clinical setting of human primary osteosarcomas and in E2F1-inducible osteosarcoma cell line models that are wild-type and deficient for p53. Collectively, our data demonstrated that high E2F1 levels exerted a growth-suppressing effect that relied on the integrity of the DNA damage response network...

  9. Visceral anatomy of ocean sunfish (Mola mola (L., 1758), Molidae, Tetraodontiformes) and angler (Lophius piscatorius (L., 1758), Lophiidae, Lophiiformes) investigated by non-invasive imaging techniques.

    Science.gov (United States)

    Chanet, Bruno; Guintard, Claude; Boisgard, Thierry; Fusellier, Marion; Tavernier, Cédric; Betti, Eric; Madec, Stéphane; Richaudeau, Yvan; Raphaël, Christian; Dettaï, Agnès; Lecointre, Guillaume

    2012-12-01

    The purpose of this work is to examine the gross visceral anatomy of ocean sunfish and angler using non-invasive imaging techniques: computed tomography imaging (CT) and magnetic resonance imaging (MRI). Similarities and differences in the internal organisation of these two species are verified. Both species lack a swimbladder and present a significant asymmetry in the hepatic lobes, an elongated bile duct terminating close to the stomach, a compact thyroid embedded in a blood lacuna, and very reduced brain and spinal cord. These observations are important in regard to the close relationships between Tetraodontiformes and Lophiiformes, established by several molecular works, but not yet confirmed by morpho-anatomical data. However the occurrence of these features has to be examined in other taxa before phylogenetic hypotheses are proposed. Copyright © 2012 Académie des sciences. Published by Elsevier SAS. All rights reserved.

  10. Occurrence of S and F1C/S-related fimbrial determinants and their expression in Escherichia coli strains isolated from extraintestinal infections.

    Science.gov (United States)

    Sokolowska-Köhler, W; Schönian, G; Bollmann, R; Schubert, A; Parschau, J; Seeberg, A; Presber, W

    1997-05-01

    The presence of S and F1C/S-related fimbrial determinants was determined in 462 E. coli strains obtained from different extraintestinal infections and in 162 control isolates of E. coli by using two different DNA probes: an oligonucleotide probe consisting of three oligonucleotides that bind specifically to the S adhesin gene and a polynucleotide probe which is not able to distinguish between S, F1C, and S-related sequences. The expression of S and F1C phenotypes was tested by dot enzyme immunoassay with the corresponding monoclonal antibodies. S fimbriae genotypes were observed more frequently in septic (25%) and urinary (12%) isolates of E. coli than in faecal and water isolates (1%) and often occurred together with O2, O6, O18 and O83 antigens. F1C/S-related fimbrial DNA was detected with a higher frequency in UTI isolates (26%) than in septic (16%) and faecal (10%) isolates and was most frequently associated with O4, O6, and O75 serotypes. Since the production of S and F1C fimbriae was comparatively rare in all clinical and control isolates of E. coli, DNA hybridization assays which allow the sensitive and specific detection of fimbrial determinants even in the absence of their expression are preferable to phenotypic assays.

  11. Relevance of unilateral and bilateral sexual polyploidization in relation to intergenomic recombination and introgression in Lilium species hybrids

    NARCIS (Netherlands)

    Nadeem Khan, M.; Barba Gonzalez, R.; Ramanna, M.S.; Arens, P.F.P.; Visser, R.G.F.; Tuyl, van J.M.

    2010-01-01

    Sexual polyploids were induced in diploid (2n = 2x = 24) interspecific F1 hybrids of Longiflorum × Asiatic (LA) and Oriental × Asiatic (OA) Lilium hybrids by backcrossing to Asiatic (AA) parents as well as by sib-mating of the F1 LA hybrids. A majority of the BC1 progenies were triploid and the prog

  12. Integration of F1 and the membrane sector of the proton-ATPase of Escherichia coli. Role of subunit "b" (uncF protein).

    Science.gov (United States)

    Perlin, D S; Cox, D N; Senior, A E

    1983-08-25

    Membranes derived from the Escherichia coli strain AN1460 which carries the multicopy plasmid pAN45 (unc+) (Downie, J. A., Langman, L., Cox, G. B., Yanofsky, C., and Gibson, (1980) J. Bacteriol. 143, 8-17) were enriched 5- to 10-fold in proton-ATPase activity. Incubation of F1-depleted AN1460 membranes with trypsin abolished F1-binding ability but did not inhibit proton transport through the membrane sector (F0). Sodium dodecyl sulfate-gel electrophoresis indicated that subunit "b" (uncF protein) of F0 was cleaved by trypsin and prebound F1 protected against the trypsin effect. Subunits "a" (uncB protein) and "c" (uncE protein) were unaffected by the trypsin treatment. A water-soluble fragment (Mr = 14,800) was liberated after trypsin treatment and appeared to arise from subunit b. Studies of enzyme hybridization and of F1 binding to membranes derived from strains containing mutations in uncB, F, and E genes supported the suggestion that subunit b is involved in F1 binding to the F0. Also, extraction of membranes with KSCN increased the relative proportion of subunit b in the membrane and this coincided with a parallel increase in trypsin-sensitive F1-binding ability. It is proposed that subunit b is involved in binding of F1 to the F0; this agrees with the presumed role of the protein as deduced from predictions of its secondary and tertiary structure (Walker, J. E., Saraste, M., and Gay, N. J. (1982) Nature (Lond.) 298, 867-869; Senior, A. E. (1983) Biochim. Biophys. Acta, in press).

  13. The nonlinear chemo-mechanic coupled dynamics of the F 1 -ATPase molecular motor.

    Science.gov (United States)

    Xu, Lizhong; Liu, Fang

    2012-03-01

    The ATP synthase consists of two opposing rotary motors, F0 and F1, coupled to each other. When the F1 motor is not coupled to the F0 motor, it can work in the direction hydrolyzing ATP, as a nanomotor called F1-ATPase. It has been reported that the stiffness of the protein varies nonlinearly with increasing load. The nonlinearity has an important effect on the rotating rate of the F1-ATPase. Here, considering the nonlinearity of the γ shaft stiffness for the F1-ATPase, a nonlinear chemo-mechanical coupled dynamic model of F1 motor is proposed. Nonlinear vibration frequencies of the γ shaft and their changes along with the system parameters are investigated. The nonlinear stochastic response of the elastic γ shaft to thermal excitation is analyzed. The results show that the stiffness nonlinearity of the γ shaft causes an increase of the vibration frequency for the F1 motor, which increases the motor's rotation rate. When the concentration of ATP is relatively high and the load torque is small, the effects of the stiffness nonlinearity on the rotating rates of the F1 motor are obvious and should be considered. These results are useful for improving calculation of the rotating rate for the F1 motor and provide insight about the stochastic wave mechanics of F1-ATPase.

  14. Hybridization and the evolution of reef coral diversity.

    Science.gov (United States)

    Vollmer, Steven V; Palumbi, Stephen R

    2002-06-14

    Hundreds of coral species coexist sympatrically on reefs, reproducing in mass-spawning events where hybridization appears common. In the Caribbean, DNA sequence data from all three sympatric Acropora corals show that mass spawning does not erode species barriers. Species A. cervicornis and A. palmata are distinct at two nuclear loci or share ancestral alleles. Morphotypes historically given the name Acropora prolifera are entirely F(1) hybrids of these two species, showing morphologies that depend on which species provides the egg for hybridization. Although selection limits the evolutionary potential of hybrids, F(1) individuals can reproduce asexually and form long-lived, potentially immortal hybrids with unique morphologies.

  15. The tumor suppressor gene hypermethylated in cancer 1 is transcriptionally regulated by E2F1

    DEFF Research Database (Denmark)

    Jenal, Mathias; Trinh, Emmanuelle; Britschgi, Christian;

    2009-01-01

    The Hypermethylated in Cancer 1 (HIC1) gene encodes a zinc finger transcriptional repressor that cooperates with p53 to suppress cancer development. We and others recently showed that HIC1 is a transcriptional target of p53. To identify additional transcriptional regulators of HIC1, we screened...... to the HIC1 promoter was shown by chromatin immunoprecipitation assays in human TIG3 fibroblasts expressing tamoxifen-activated E2F1. In agreement, activation of E2F1 in TIG3-E2F1 cells markedly increased HIC1 expression. Interestingly, expression of E2F1 in the p53(-/-) hepatocellular carcinoma cell line...

  16. E. coli F1-ATPase: site-directed mutagenesis of the beta-subunit.

    Science.gov (United States)

    Parsonage, D; Wilke-Mounts, S; Senior, A E

    1988-05-09

    Residues beta Glu-181 and beta Glu-192 of E. coli F1-ATPase (the DCCD-reactive residues) were mutated to Gln. Purified beta Gln-181 F1 showed 7-fold impairment of 'unisite' Pi formation from ATP and a large decrease in affinity for ATP. Thus the beta-181 carboxyl group in normal F1 significantly contributes to catalytic site properties. Also, positive catalytic site cooperativity was attenuated from 5 X 10(4)- to 548-fold in beta Gln-181 F1. In contrast, purified beta Gln-192 F1 showed only 6-fold reduction in 'multisite' ATPase activity. Residues beta Gly-149 and beta Gly-154 were mutated to Ile singly and in combination. These mutations, affecting residues which are strongly conserved in nucleotide-binding proteins, were chosen to hinder conformational motion in a putative 'flexible loop' in beta-subunit. Impairment of purified F1-ATPase ranged from 5 to 61%, with the double mutant F1 less impaired than either single mutant. F1 preparations containing beta Ile-154 showed 2-fold activation after release from membranes, suggesting association with F0 restrained turnover on F1 in these mutants.

  17. Clustering of conformational IgE epitopes on the major dog allergen Can f 1.

    Science.gov (United States)

    Curin, Mirela; Weber, Milena; Hofer, Gerhard; Apostolovic, Danijela; Keller, Walter; Reininger, Renate; Swoboda, Ines; Spitzauer, Susanne; Focke-Tejkl, Margit; van Hage, Marianne; Valenta, Rudolf

    2017-09-22

    Immunoglobulin E (IgE)-associated allergy affects more than 25% of the population. Can f 1 is the major dog allergen associated with respiratory symptoms but the epitopes recognized by allergic patients IgE on Can f 1 are unknown. To characterize IgE epitopes of Can f 1 recognized by dog allergic patients, six overlapping peptides spanning the Can f 1 sequence were synthesized. In direct IgE epitope mapping experiments peptides were analyzed for IgE reactivity by dot blot and Enzyme-linked immunosorbent assay (ELISA) with sera from dog allergic patients. For indirect epitope-mapping, rabbits were immunized with the peptides to generate specific IgG antibodies which were used to inhibit allergic patients' IgE binding to Can f 1. IgE binding sites were visualized on a model of the Can f 1 three-dimensional structure. We found that Can f 1 does not contain any relevant sequential IgE epitopes. However, IgE inhibition experiments with anti-peptide specific IgGs showed that Can f 1 N- and C-terminal portion assembled a major conformational binding site. In conclusion, our study is the first to identify the major IgE epitope-containing area of the dog allergen Can f 1. This finding is important for the development of allergen-specific treatment strategies.

  18. E2F1 transcription factor and its impact on growth factor and cytokine signaling.

    Science.gov (United States)

    Ertosun, Mustafa Gokhan; Hapil, Fatma Zehra; Osman Nidai, Ozes

    2016-10-01

    E2F1 is a transcription factor involved in cell cycle regulation and apoptosis. The transactivation capacity of E2F1 is regulated by pRb. In its hypophosphorylated form, pRb binds and inactivates DNA binding and transactivating functions of E2F1. The growth factor stimulation of cells leads to activation of CDKs (cyclin dependent kinases), which in turn phosphorylate Rb and hyperphosphorylated Rb is released from E2F1 or E2F1/DP complex, and free E2F1 can induce transcription of several genes involved in cell cycle entry, induction or inhibition of apoptosis. Thus, growth factors and cytokines generally utilize E2F1 to direct cells to either fate. Furthermore, E2F1 regulates expressions of various cytokines and growth factor receptors, establishing positive or negative feedback mechanisms. This review focuses on the relationship between E2F1 transcription factor and cytokines (IL-1, IL-2, IL-3, IL-6, TGF-beta, G-CSF, LIF), growth factors (EGF, KGF, VEGF, IGF, FGF, PDGF, HGF, NGF), and interferons (IFN-α, IFN-β and IFN-γ).

  19. Polyploidy Enhances F1 Pollen Sterility Loci Interactions That Increase Meiosis Abnormalities and Pollen Sterility in Autotetraploid Rice1[OPEN

    Science.gov (United States)

    Wu, Jinwen; Chen, Lin; Chen, Zhixiong; Wang, Lan; Lu, Yonggen

    2015-01-01

    Intersubspecific autotetraploid rice (Oryza sativa ssp. indica × japonica) hybrids have greater biological and yield potentials than diploid rice. However, the low fertility of intersubspecific autotetraploid hybrids, which is largely caused by high pollen abortion rates, limits their commercial utility. To decipher the cytological and molecular mechanisms underlying allelic interactions in autotetraploid rice, we developed an autotetraploid rice hybrid that was heterozygous (SiSj) at F1 pollen sterility loci (Sa, Sb, and Sc) using near-isogenic lines. Cytological studies showed that the autotetraploid had higher percentages (>30%) of abnormal chromosome behavior and aberrant meiocytes (>50%) during meiosis than did the diploid rice hybrid control. Analysis of gene expression profiles revealed 1,888 genes that were differentially expressed between the autotetraploid and diploid hybrid lines at the meiotic stage, among which 889 and 999 were up- and down-regulated, respectively. Of the 999 down-regulated genes, 940 were associated with the combined effect of polyploidy and pollen sterility loci interactions (IPE). Gene Ontology enrichment analysis identified a prominent functional gene class consisting of seven genes related to photosystem I (Gene Ontology 0009522). Moreover, 55 meiosis-related or meiosis stage-specific genes were associated with IPE in autotetraploid rice, including Os02g0497500, which encodes a DNA repair-recombination protein, and Os02g0490000, which encodes a component of the ubiquitin-proteasome pathway. These results suggest that polyploidy enhances epistatic interactions between alleles of pollen sterility loci, thereby altering the expression profiles of important meiosis-related or meiosis stage-specific genes and resulting in high pollen sterility. PMID:26511913

  20. Polyploidy Enhances F1 Pollen Sterility Loci Interactions That Increase Meiosis Abnormalities and Pollen Sterility in Autotetraploid Rice.

    Science.gov (United States)

    Wu, Jinwen; Shahid, Muhammad Qasim; Chen, Lin; Chen, Zhixiong; Wang, Lan; Liu, Xiangdong; Lu, Yonggen

    2015-12-01

    Intersubspecific autotetraploid rice (Oryza sativa ssp. indica × japonica) hybrids have greater biological and yield potentials than diploid rice. However, the low fertility of intersubspecific autotetraploid hybrids, which is largely caused by high pollen abortion rates, limits their commercial utility. To decipher the cytological and molecular mechanisms underlying allelic interactions in autotetraploid rice, we developed an autotetraploid rice hybrid that was heterozygous (S(i)S(j)) at F1 pollen sterility loci (Sa, Sb, and Sc) using near-isogenic lines. Cytological studies showed that the autotetraploid had higher percentages (>30%) of abnormal chromosome behavior and aberrant meiocytes (>50%) during meiosis than did the diploid rice hybrid control. Analysis of gene expression profiles revealed 1,888 genes that were differentially expressed between the autotetraploid and diploid hybrid lines at the meiotic stage, among which 889 and 999 were up- and down-regulated, respectively. Of the 999 down-regulated genes, 940 were associated with the combined effect of polyploidy and pollen sterility loci interactions (IPE). Gene Ontology enrichment analysis identified a prominent functional gene class consisting of seven genes related to photosystem I (Gene Ontology 0009522). Moreover, 55 meiosis-related or meiosis stage-specific genes were associated with IPE in autotetraploid rice, including Os02g0497500, which encodes a DNA repair-recombination protein, and Os02g0490000, which encodes a component of the ubiquitin-proteasome pathway. These results suggest that polyploidy enhances epistatic interactions between alleles of pollen sterility loci, thereby altering the expression profiles of important meiosis-related or meiosis stage-specific genes and resulting in high pollen sterility. © 2015 American Society of Plant Biologists. All Rights Reserved.

  1. Ploidy manipulation and introgression breeding in Darwin hybrid tulips

    NARCIS (Netherlands)

    Marasek-Ciolakowska, A.; Xie, S.L.; Arens, P.; Tuyl, van J.M.

    2014-01-01

    Meiotic polyploidisation via crossing with 2n gamete producing genotypes and interploidy crosses are two of the main methods currently used to obtain polyploid tulips. In our study diploid 2n gamete producing F-1 hybrids of Darwin hybrids (Tulipa gesneriana x Tulipa fosteriana) and triploid hybrid r

  2. The polar domain of the b subunit of Escherichia coli F1F0-ATPase forms an elongated dimer that interacts with the F1 sector.

    Science.gov (United States)

    Dunn, S D

    1992-04-15

    A soluble form of the b subunit of the F0 sector of the F1F0-ATPase of Escherichia coli has been produced, purified, and characterized. In this form of the protein, designated bsol, residues 25-146 (the carboxyl terminus) of b have been fused to an amino-terminal octapeptide extension derived from the vector pUC8. The inferred subunit molecular weight of bsol is 15,459. bsol protein was expressed in E. coli as a soluble cytoplasmic protein and was readily purified to homogeneity by conventional methods. The molecular weight of bsol, determined by sedimentation equilibrium, was 31,200, indicating that the protein is dimeric. Chemical cross-linking studies supported this conclusion. However, bsol sedimented with a coefficient of just 1.8 S and behaved on size exclusion chromatography with an apparent molecular weight of 80,000-85,000. These results indicate that the protein exists in solution as a highly elongated dimer. The circular dichroism spectrum indicated that bsol is highly alpha-helical. Binding of bsol to F1-ATPase was directly demonstrated by size exclusion chromatography. bsol also inhibited the binding of F1-ATPase to F1-depleted membrane vesicles, as measured by reconstitution of energy-dependent quinacrine fluorescence quenching. This result implies that bsol and F0 compete for binding to the same site on F1. The apparently normal interaction of bsol with F1-ATPase strongly suggests that the recombinant protein assumes the correct structure. No substantial effects of bsol on the ATPase activity of purified F1 were observed.

  3. Cloning and Characterization of an mRNA Encoding F1-ATPase Beta-Subunit Abundant in Epithelial Cells of Mantle and Gill of Pearl Oyster, Pinctadafucata

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In oyster biomineralization, large amounts of calcium are absorbed from external media, transported to the mineralization site, and finally deposited via a matrix-mediated process. All these activities are very energy intensive; therefore, investigations of the energy metabolism pathways of different oyster tissues will facilitate understanding of oyster biomineralization physiology. A full-length cDNA encoding the F1-ATPase beta-subunit (the F1-β-subunit, a major calalytic subunit of F-ATPase) from the pearl oyster (Pinctads fucata) was cloned using the homology strategy with a pair of degenerated primers based on the conserved regions of other animals' F1-β-subunit genes. Sequencing and structural analyses showed that the obtained sequence shared high identity with other animals' F1-β-subunits, and had a unique phosphorylation site of PKC and CK Ⅱ on the external surface of the putative protein. Results from semi-quantitative reverse transcription-polymerase chain reaction and in situ hybridization demonstrated this oyster F1-β-subunit mRNA is abundant in the gill and mantle, and distributed widely in the periostracal groove, the outer folder,and the dorsal region of the mantle and in the gill epithelial cells. These tissues were the main regions that participate in biomineralization processes such as calcium uptake, transport, and matrix secretion. The results indicate that tissues involved in biomineralization have stronger energy metabolic processes and that F1-ATPase might play an important role in oyster biomineralization by providing energy transport.

  4. Characterization of an F1 Deletion Mutant of Yersinia pestis CO92, Pathogenic Role of F1 Antigen in Bubonic and Pneumonic Plague, and Evaluation of Sensitivity and Specificity of F1 Antigen Capture-Based Dipsticks▿

    Science.gov (United States)

    Sha, Jian; Endsley, Janice J.; Kirtley, Michelle L.; Foltz, Sheri M.; Huante, Matthew B.; Erova, Tatiana E.; Kozlova, Elena V.; Popov, Vsevolod L.; Yeager, Linsey A.; Zudina, Irina V.; Motin, Vladimir L.; Peterson, Johnny W.; DeBord, Kristin L.; Chopra, Ashok K.

    2011-01-01

    We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipsticks, an in-frame F1-deficient mutant of CO92 (Δcaf) was generated by homologous recombination and used as a negative control. Based on genetic, antigenic/immunologic, and electron microscopic analyses, the Δcaf mutant was devoid of a capsule. The growth rate of the Δcaf mutant generally was similar to that of the wild-type (WT) bacterium at both 26 and 37°C, although the mutant's growth dropped slightly during the late phase at 37°C. The Δcaf mutant was as virulent as WT CO92 in the pneumonic plague mouse model; however, it was attenuated in developing bubonic plague. Both dipsticks had similar sensitivities, requiring a minimum of 0.5 μg/ml of purified F1 antigen or 1 × 105 to 5 × 105 CFU/ml of WT CO92 for positive results, while the blood samples were negative for up to 1 × 108 CFU/ml of the Δcaf mutant. Our studies demonstrated the diagnostic potential of two plague dipsticks in detecting capsular-positive strains of Y. pestis in bubonic and pneumonic plague. PMID:21367990

  5. 26 CFR 5c.44F-1 - Leases and qualified research expenses.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 14 2010-04-01 2010-04-01 false Leases and qualified research expenses. 5c.44F-1 Section 5c.44F-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) TEMPORARY INCOME TAX REGULATIONS UNDER THE ECONOMIC RECOVERY TAX ACT OF 1981 § 5c...

  6. Catalytic properties of Escherichia coli F1-ATPase depleted of endogenous nucleotides.

    Science.gov (United States)

    Senior, A E; Lee, R S; al-Shawi, M K; Weber, J

    1992-09-01

    Nucleotide-depleted Escherichia coli F1 was prepared by the procedure of Wise et al. (1983, Biochem. J. 215, 343-350). This enzyme had high rates of steady-state ATPase and GTPase activity. When "unisite" ATP hydrolysis was measured using an F1/ATP concentration ratio of 10, all of the substoichiometric ATP became bound to the high-affinity catalytic site and none became bound to noncatalytic sites. The association rate constant for ATP binding was 7 x 10(5) M-1 s-1 and the KdATP was 7.9 x 10(-10) M, as compared to values of 3.8 x 10(5) M-1 s-1 and 1.9 x 10(-10) M, respectively, in native (i.e., nucleotide-replete) F1. Rate constants for bound ATP hydrolysis, ATP resynthesis, and P(i) release, and the reaction equilibrium constant, were similar in nucleotide-depleted and native F1. Therefore, we conclude that occupancy of the noncatalytic sites is not required for formation of the high-affinity catalytic site of F1 and has no significant effect on unisite catalysis. In further experiments we looked for the occurrence of inhibitory, catalytic-site-bound MgADP in E. coli F1. Such an entity has been reported for chloroplast and mitochondrial F1. However, our experiments gave no indication for inhibitory MgADP in E. coli F1.

  7. E2F-1-Induced p53-independent apoptosis in transgenic mice

    DEFF Research Database (Denmark)

    Holmberg, Christian Henrik; Helin, K.; Sehested, M.;

    1998-01-01

    involving increased apoptosis in the germinal epithelium. This effect was potentiated by simultaneous overexpression of DP-1. Testicular atrophy as a result of overexpression of E2F-1 and DP-1 is independent of functional p53, since p53-nullizygous transgenic mice overexpressing E2F-1 and DP-1 also suffered...

  8. 26 CFR 1.665(f)-1A - Undistributed capital gain.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Undistributed capital gain. 1.665(f)-1A Section... Beginning on Or After January 1, 1969 § 1.665(f)-1A Undistributed capital gain. (a) Domestic trusts. (1) The term undistributed capital gain means (in the case of a trust other than a foreign trust created by a...

  9. 26 CFR 1.514(f)-1 - Definition of business lease.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Definition of business lease. 1.514(f)-1 Section... § 1.514(f)-1 Definition of business lease. (a) In general. The term business lease means any lease...) if at the close of the organization's taxable year there is a business lease indebtedness as...

  10. Sterile Insect Technique and F1 Sterility in the European Grapevine Moth, Lobesia botrana

    Science.gov (United States)

    Saour, George

    2014-01-01

    Newly emerged adults of the European grapevine moth, Lobesia botrana (Denis and Schiffermuller) (Lepidoptera: Tortricidae), were irradiated with various doses of gamma radiation and crossed to unirradiated counterparts of the opposite sex. Fecundity was decreased when unirradiated females were mated with either 300- or 350-Gy-irradiated males. Adult males that were irradiated with 400 Gy and mated with unirradiated females retained a residual fertility of 2.7%. The radiation dose at which irradiated females were found to be 100% sterile when mated with unirradiated males was 150 Gy. The inherited effects in the F1 progeny of irradiated male parents were examined at 100, 150, and 200 Gy. Fecundity and fertility of the F1 progeny of males irradiated with 150 Gy and inbred or crossed with irradiated and unirradiated moths were also recorded. A significant reduction in fertility was observed when F1 males mated with either F1 or unirradiated females. According to sterility index, F1 females who mated with F1 males had greater sterility than when F1 females were crossed to 150-Gy-irradiated males. Based upon the results of this study, 150 Gy of gamma radiation would be the optimal dose to use in a sterile insect technique and F1 sterility program against L. botrana. PMID:25373155

  11. 77 FR 20038 - Employment Authorization for Syrian F-1 Nonimmigrant Students Experiencing Severe Economic...

    Science.gov (United States)

    2012-04-03

    ... minimum course load requirement, unless the student's course of study is in a language study program. See... maintains his or her TPS, then the student maintains F-1 status and TPS concurrently. Under the second... SECURITY RIN 1653-ZA04 Employment Authorization for Syrian F-1 Nonimmigrant Students Experiencing...

  12. 26 CFR 301.6501(f)-1 - Personal holding company tax.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Personal holding company tax. 301.6501(f)-1... Collection § 301.6501(f)-1 Personal holding company tax. If a corporation which is a personal holding company... time during the last half of such taxable year, more than 50 percent in value of the...

  13. Observation of $\\bar{B}^0_{(s)}\\rightarrow J/\\psi f_1(1285)$ decays and measurement of the $f_1(1285)$ mixing angle

    CERN Document Server

    Aaij, R; Adinolfi, M; Adrover, C; Affolder, A; Ajaltouni, Z; Albrecht, J; Alessio, F; Alexander, M; Ali, S; Alkhazov, G; Alvarez Cartelle, P; Alves Jr, A A; Amato, S; Amerio, S; Amhis, Y; Anderlini, L; Anderson, J; Andreassen, R; Andreotti, M; Andrews, J E; Appleby, R B; Aquines Gutierrez, O; Archilli, F; Artamonov, A; Artuso, M; Aslanides, E; Auriemma, G; Baalouch, M; Bachmann, S; Back, J J; Badalov, A; Baesso, C; Balagura, V; Baldini, W; Barlow, R J; Barschel, C; Barsuk, S; Barter, W; Batozskaya, V; Bauer, Th; Bay, A; Beddow, J; Bedeschi, F; Bediaga, I; Belogurov, S; Belous, K; Belyaev, I; Ben-Haim, E; Bencivenni, G; Benson, S; Benton, J; Berezhnoy, A; Bernet, R; Bettler, M -O; van Beuzekom, M; Bien, A; Bifani, S; Bird, T; Bizzeti, A; Bjørnstad, P M; Blake, T; Blanc, F; Blouw, J; Blusk, S; Bocci, V; Bondar, A; Bondar, N; Bonivento, W; Borghi, S; Borgia, A; Bowcock, T J V; Bowen, E; Bozzi, C; Brambach, T; van den Brand, J; Bressieux, J; Brett, D; Britsch, M; Britton, T; Brook, N H; Brown, H; Bursche, A; Busetto, G; Buytaert, J; Cadeddu, S; Calabrese, R; Callot, O; Calvi, M; Calvo Gomez, M; Camboni, A; Campana, P; Campora Perez, D; Carbone, A; Carboni, G; Cardinale, R; Cardini, A; Carranza-Mejia, H; Carson, L; Carvalho Akiba, K; Casse, G; Castillo Garcia, L; Cattaneo, M; Cauet, Ch; Cenci, R; Charles, M; Charpentier, Ph; Cheung, S -F; Chiapolini, N; Chrzaszcz, M; Ciba, K; Cid Vidal, X; Ciezarek, G; Clarke, P E L; Clemencic, M; Cliff, H V; Closier, J; Coca, C; Coco, V; Cogan, J; Cogneras, E; Collins, P; Comerma-Montells, A; Contu, A; Cook, A; Coombes, M; Coquereau, S; Corti, G; Couturier, B; Cowan, G A; Craik, D C; Cruz Torres, M; Cunliffe, S; Currie, R; D'Ambrosio, C; David, P; David, P N Y; Davis, A; De Bonis, I; De Bruyn, K; De Capua, S; De Cian, M; De Miranda, J M; De Paula, L; De Silva, W; De Simone, P; Decamp, D; Deckenhoff, M; Del Buono, L; Déléage, N; Derkach, D; Deschamps, O; Dettori, F; Di Canto, A; Dijkstra, H; Dogaru, M; Donleavy, S; Dordei, F; Dosil Suárez, A; Dossett, D; Dovbnya, A; Dupertuis, F; Durante, P; Dzhelyadin, R; Dziurda, A; Dzyuba, A; Easo, S; Egede, U; Egorychev, V; Eidelman, S; van Eijk, D; Eisenhardt, S; Eitschberger, U; Ekelhof, R; Eklund, L; El Rifai, I; Elsasser, Ch; Falabella, A; Färber, C; Farinelli, C; Farry, S; Ferguson, D; Fernandez Albor, V; Ferreira Rodrigues, F; Ferro-Luzzi, M; Filippov, S; Fiore, M; Fiorini, M; Fitzpatrick, C; Fontana, M; Fontanelli, F; Forty, R; Francisco, O; Frank, M; Frei, C; Frosini, M; Furfaro, E; Gallas Torreira, A; Galli, D; Gandelman, M; Gandini, P; Gao, Y; Garofoli, J; Garosi, P; Garra Tico, J; Garrido, L; Gaspar, C; Gauld, R; Gersabeck, E; Gersabeck, M; Gershon, T; Ghez, Ph; Gibson, V; Giubega, L; Gligorov, V V; Göbel, C; Golubkov, D; Golutvin, A; Gomes, A; Gorbounov, P; Gordon, H; Grabalosa Gándara, M; Graciani Diaz, R; Granado Cardoso, L A; Graugés, E; Graziani, G; Grecu, A; Greening, E; Gregson, S; Griffith, P; Grillo, L; Grünberg, O; Gui, B; Gushchin, E; Guz, Yu; Gys, T; Hadjivasiliou, C; Haefeli, G; Haen, C; Hafkenscheid, T W; Haines, S C; Hall, S; Hamilton, B; Hampson, T; Hansmann-Menzemer, S; Harnew, N; Harnew, S T; Harrison, J; Hartmann, T; He, J; Head, T; Heijne, V; Hennessy, K; Henrard, P; Hernando Morata, J A; van Herwijnen, E; Heß, M; Hicheur, A; Hicks, E; Hill, D; Hoballah, M; Hombach, C; Hulsbergen, W; Hunt, P; Huse, T; Hussain, N; Hutchcroft, D; Hynds, D; Iakovenko, V; Idzik, M; Ilten, P; Jacobsson, R; Jaeger, A; Jans, E; Jaton, P; Jawahery, A; Jing, F; John, M; Johnson, D; Jones, C R; Joram, C; Jost, B; Kaballo, M; Kandybei, S; Kanso, W; Karacson, M; Karbach, T M; Kenyon, I R; Ketel, T; Khanji, B; Kochebina, O; Komarov, I; Koopman, R F; Koppenburg, P; Korolev, M; Kozlinskiy, A; Kravchuk, L; Kreplin, K; Kreps, M; Krocker, G; Krokovny, P; Kruse, F; Kucharczyk, M; Kudryavtsev, V; Kurek, K; Kvaratskheliya, T; La Thi, V N; Lacarrere, D; Lafferty, G; Lai, A; Lambert, D; Lambert, R W; Lanciotti, E; Lanfranchi, G; Langenbruch, C; Latham, T; Lazzeroni, C; Le Gac, R; van Leerdam, J; Lees, J -P; Lefèvre, R; Leflat, A; Lefrançois, J; Leo, S; Leroy, O; Lesiak, T; Leverington, B; Li, Y; Li Gioi, L; Liles, M; Lindner, R; Linn, C; Liu, B; Liu, G; Lohn, S; Longstaff, I; Lopes, J H; Lopez-March, N; Lu, H; Lucchesi, D; Luisier, J; Luo, H; Luppi, E; Lupton, O; Machefert, F; Machikhiliyan, I V; Maciuc, F; Maev, O; Malde, S; Manca, G; Mancinelli, G; Maratas, J; Marconi, U; Marino, P; Märki, R; Marks, J; Martellotti, G; Martens, A; Martín Sánchez, A; Martinelli, M; Martinez Santos, D; Martins Tostes, D; Martynov, A; Massafferri, A; Matev, R; Mathe, Z; Matteuzzi, C; Maurice, E; Mazurov, A; McCann, M; McCarthy, J; McNab, A; McNulty, R; McSkelly, B; Meadows, B; Meier, F; Meissner, M; Merk, M; Milanes, D A; Minard, M -N; Molina Rodriguez, J; Monteil, S; Moran, D; Morawski, P; Mordà, A; Morello, M J; Mountain, R; Mous, I; Muheim, F; Müller, K; Muresan, R; Muryn, B; Muster, B; Naik, P; Nakada, T; Nandakumar, R; Nasteva, I; Needham, M; Neubert, S; Neufeld, N; Nguyen, A D; Nguyen, T D; Nguyen-Mau, C; Nicol, M; Niess, V; Niet, R; Nikitin, N; Nikodem, T; Nomerotski, A; Novoselov, A; Oblakowska-Mucha, A; Obraztsov, V; Oggero, S; Ogilvy, S; Okhrimenko, O; Oldeman, R; Onderwater, G; Orlandea, M; Otalora Goicochea, J M; Owen, P; Oyanguren, A; Pal, B K; Palano, A; Palutan, M; Panman, J; Papanestis, A; Pappagallo, M; Parkes, C; Parkinson, C J; Passaleva, G; Patel, G D; Patel, M; Patrick, G N; Patrignani, C; Pavel-Nicorescu, C; Pazos Alvarez, A; Pearce, A; Pellegrino, A; Penso, G; Pepe Altarelli, M; Perazzini, S; Perez Trigo, E; Pérez-Calero Yzquierdo, A; Perret, P; Perrin-Terrin, M; Pescatore, L; Pesen, E; Pessina, G; Petridis, K; Petrolini, A; Phan, A; Picatoste Olloqui, E; Pietrzyk, B; Pilař, T; Pinci, D; Playfer, S; Plo Casasus, M; Polci, F; Polok, G; Poluektov, A; Polycarpo, E; Popov, A; Popov, D; Popovici, B; Potterat, C; Powell, A; Prisciandaro, J; Pritchard, A; Prouve, C; Pugatch, V; Puig Navarro, A; Punzi, G; Qian, W; Rachwal, B; Rademacker, J H; Rakotomiaramanana, B; Rangel, M S; Raniuk, I; Rauschmayr, N; Raven, G; Redford, S; Reichert, S; Reid, M M; dos Reis, A C; Ricciardi, S; Richards, A; Rinnert, K; Rives Molina, V; Roa Romero, D A; Robbe, P; Roberts, D A; Rodrigues, A B; Rodrigues, E; Rodriguez Perez, P; Roiser, S; Romanovsky, V; Romero Vidal, A; Rotondo, M; Rouvinet, J; Ruf, T; Ruffini, F; Ruiz, H; Ruiz Valls, P; Sabatino, G; Saborido Silva, J J; Sagidova, N; Sail, P; Saitta, B; Salustino Guimaraes, V; Sanmartin Sedes, B; Santacesaria, R; Santamarina Rios, C; Santovetti, E; Sapunov, M; Sarti, A; Satriano, C; Satta, A; Savrie, M; Savrina, D; Schiller, M; Schindler, H; Schlupp, M; Schmelling, M; Schmidt, B; Schneider, O; Schopper, A; Schune, M -H; Schwemmer, R; Sciascia, B; Sciubba, A; Seco, M; Semennikov, A; Senderowska, K; Sepp, I; Serra, N; Serrano, J; Seyfert, P; Shapkin, M; Shapoval, I; Shcheglov, Y; Shears, T; Shekhtman, L; Shevchenko, O; Shevchenko, V; Shires, A; Silva Coutinho, R; Sirendi, M; Skidmore, N; Skwarnicki, T; Smith, N A; Smith, E; Smith, E; Smith, J; Smith, M; Sokoloff, M D; Soler, F J P; Soomro, F; Souza, D; Souza De Paula, B; Spaan, B; Sparkes, A; Spradlin, P; Stagni, F; Stahl, S; Steinkamp, O; Stevenson, S; Stoica, S; Stone, S; Storaci, B; Straticiuc, M; Straumann, U; Subbiah, V K; Sun, L; Sutcliffe, W; Swientek, S; Syropoulos, V; Szczekowski, M; Szczypka, P; Szilard, D; Szumlak, T; T'Jampens, S; Teklishyn, M; Tellarini, G; Teodorescu, E; Teubert, F; Thomas, C; Thomas, E; van Tilburg, J; Tisserand, V; Tobin, M; Tolk, S; Tomassetti, L; Tonelli, D; Topp-Joergensen, S; Torr, N; Tournefier, E; Tourneur, S; Tran, M T; Tresch, M; Tsaregorodtsev, A; Tsopelas, P; Tuning, N; Ubeda Garcia, M; Ukleja, A; Ustyuzhanin, A; Uwer, U; Vagnoni, V; Valenti, G; Vallier, A; Vazquez Gomez, R; Vazquez Regueiro, P; Vázquez Sierra, C; Vecchi, S; Velthuis, J J; Veltri, M; Veneziano, G; Vesterinen, M; Viaud, B; Vieira, D; Vilasis-Cardona, X; Vollhardt, A; Volyanskyy, D; Voong, D; Vorobyev, A; Vorobyev, V; Voß, C; Voss, H; Waldi, R; Wallace, C; Wallace, R; Wandernoth, S; Wang, J; Ward, D R; Watson, N K; Webber, A D; Websdale, D; Whitehead, M; Wicht, J; Wiechczynski, J; Wiedner, D; Wiggers, L; Wilkinson, G; Williams, M P; Williams, M; Wilson, F F; Wimberley, J; Wishahi, J; Wislicki, W; Witek, M; Wormser, G; Wotton, S A; Wright, S; Wu, S; Wyllie, K; Xie, Y; Xing, Z; Yang, Z; Yuan, X; Yushchenko, O; Zangoli, M; Zavertyaev, M; Zhang, F; Zhang, L; Zhang, W C; Zhang, Y; Zhelezov, A; Zhokhov, A; Zhong, L; Zvyagin, A

    2014-01-01

    Decays of $\\bar{B}^0_(s)$ and $\\bar{B}^0$ mesons into $J/\\psi \\pi^+\\pi^-\\pi^+\\pi^-$ final states, produced in $pp$ collisions at the LHC, are investigated using data corresponding to an integrated luminosity of 3 fb$^{-1}$ collected with the LHCb detector. $\\bar{B}^0_{(s)}\\to J/\\psi f_1(1285)$ decays are seen for the first time, and the branching fractions are measured. Using these rates, the $f_1(1285)$ mixing angle between strange and non-strange components of its wave function in the $q\\overline{q}$ structure model is determined to be $\\pm(24.0^{\\,+3.1\\,+0.6}_{\\,-2.6\\,-0.8})^{\\circ}$. Implications on the possible tetraquark nature of the $f_1(1285)$ are discussed.

  14. Observation of B(s)(0) → J/ψ f1(1285) decays and measurement of the f1(1285) mixing angle.

    Science.gov (United States)

    Aaij, R; Adeva, B; Adinolfi, M; Adrover, C; Affolder, A; Ajaltouni, Z; Albrecht, J; Alessio, F; Alexander, M; Ali, S; Alkhazov, G; Alvarez Cartelle, P; Alves, A A; Amato, S; Amerio, S; Amhis, Y; Anderlini, L; Anderson, J; Andreassen, R; Andreotti, M; Andrews, J E; Appleby, R B; Aquines Gutierrez, O; Archilli, F; Artamonov, A; Artuso, M; Aslanides, E; Auriemma, G; Baalouch, M; Bachmann, S; Back, J J; Badalov, A; Baesso, C; Balagura, V; Baldini, W; Barlow, R J; Barschel, C; Barsuk, S; Barter, W; Batozskaya, V; Bauer, Th; Bay, A; Beddow, J; Bedeschi, F; Bediaga, I; Belogurov, S; Belous, K; Belyaev, I; Ben-Haim, E; Bencivenni, G; Benson, S; Benton, J; Berezhnoy, A; Bernet, R; Bettler, M-O; van Beuzekom, M; Bien, A; Bifani, S; Bird, T; Bizzeti, A; Bjørnstad, P M; Blake, T; Blanc, F; Blouw, J; Blusk, S; Bocci, V; Bondar, A; Bondar, N; Bonivento, W; Borghi, S; Borgia, A; Bowcock, T J V; Bowen, E; Bozzi, C; Brambach, T; van den Brand, J; Bressieux, J; Brett, D; Britsch, M; Britton, T; Brook, N H; Brown, H; Bursche, A; Busetto, G; Buytaert, J; Cadeddu, S; Calabrese, R; Callot, O; Calvi, M; Calvo Gomez, M; Camboni, A; Campana, P; Campora Perez, D; Carbone, A; Carboni, G; Cardinale, R; Cardini, A; Carranza-Mejia, H; Carson, L; Carvalho Akiba, K; Casse, G; Castillo Garcia, L; Cattaneo, M; Cauet, Ch; Cenci, R; Charles, M; Charpentier, Ph; Cheung, S-F; Chiapolini, N; Chrzaszcz, M; Ciba, K; Cid Vidal, X; Ciezarek, G; Clarke, P E L; Clemencic, M; Cliff, H V; Closier, J; Coca, C; Coco, V; Cogan, J; Cogneras, E; Collins, P; Comerma-Montells, A; Contu, A; Cook, A; Coombes, M; Coquereau, S; Corti, G; Couturier, B; Cowan, G A; Craik, D C; Cruz Torres, M; Cunliffe, S; Currie, R; D'Ambrosio, C; David, P; David, P N Y; Davis, A; De Bonis, I; De Bruyn, K; De Capua, S; De Cian, M; De Miranda, J M; De Paula, L; De Silva, W; De Simone, P; Decamp, D; Deckenhoff, M; Del Buono, L; Déléage, N; Derkach, D; Deschamps, O; Dettori, F; Di Canto, A; Dijkstra, H; Dogaru, M; Donleavy, S; Dordei, F; Dosil Suárez, A; Dossett, D; Dovbnya, A; Dupertuis, F; Durante, P; Dzhelyadin, R; Dziurda, A; Dzyuba, A; Easo, S; Egede, U; Egorychev, V; Eidelman, S; van Eijk, D; Eisenhardt, S; Eitschberger, U; Ekelhof, R; Eklund, L; El Rifai, I; Elsasser, Ch; Falabella, A; Färber, C; Farinelli, C; Farry, S; Ferguson, D; Fernandez Albor, V; Ferreira Rodrigues, F; Ferro-Luzzi, M; Filippov, S; Fiore, M; Fiorini, M; Fitzpatrick, C; Fontana, M; Fontanelli, F; Forty, R; Francisco, O; Frank, M; Frei, C; Frosini, M; Furfaro, E; Gallas Torreira, A; Galli, D; Gandelman, M; Gandini, P; Gao, Y; Garofoli, J; Garosi, P; Garra Tico, J; Garrido, L; Gaspar, C; Gauld, R; Gersabeck, E; Gersabeck, M; Gershon, T; Ghez, Ph; Gibson, V; Giubega, L; Gligorov, V V; Göbel, C; Golubkov, D; Golutvin, A; Gomes, A; Gorbounov, P; Gordon, H; Grabalosa Gándara, M; Graciani Diaz, R; Granado Cardoso, L A; Graugés, E; Graziani, G; Grecu, A; Greening, E; Gregson, S; Griffith, P; Grillo, L; Grünberg, O; Gui, B; Gushchin, E; Guz, Yu; Gys, T; Hadjivasiliou, C; Haefeli, G; Haen, C; Hafkenscheid, T W; Haines, S C; Hall, S; Hamilton, B; Hampson, T; Hansmann-Menzemer, S; Harnew, N; Harnew, S T; Harrison, J; Hartmann, T; He, J; Head, T; Heijne, V; Hennessy, K; Henrard, P; Hernando Morata, J A; van Herwijnen, E; Heß, M; Hicheur, A; Hicks, E; Hill, D; Hoballah, M; Hombach, C; Hulsbergen, W; Hunt, P; Huse, T; Hussain, N; Hutchcroft, D; Hynds, D; Iakovenko, V; Idzik, M; Ilten, P; Jacobsson, R; Jaeger, A; Jans, E; Jaton, P; Jawahery, A; Jing, F; John, M; Johnson, D; Jones, C R; Joram, C; Jost, B; Kaballo, M; Kandybei, S; Kanso, W; Karacson, M; Karbach, T M; Kenyon, I R; Ketel, T; Khanji, B; Kochebina, O; Komarov, I; Koopman, R F; Koppenburg, P; Korolev, M; Kozlinskiy, A; Kravchuk, L; Kreplin, K; Kreps, M; Krocker, G; Krokovny, P; Kruse, F; Kucharczyk, M; Kudryavtsev, V; Kurek, K; Kvaratskheliya, T; La Thi, V N; Lacarrere, D; Lafferty, G; Lai, A; Lambert, D; Lambert, R W; Lanciotti, E; Lanfranchi, G; Langenbruch, C; Latham, T; Lazzeroni, C; Le Gac, R; van Leerdam, J; Lees, J-P; Lefèvre, R; Leflat, A; Lefrançois, J; Leo, S; Leroy, O; Lesiak, T; Leverington, B; Li, Y; Li Gioi, L; Liles, M; Lindner, R; Linn, C; Liu, B; Liu, G; Lohn, S; Longstaff, I; Lopes, J H; Lopez-March, N; Lu, H; Lucchesi, D; Luisier, J; Luo, H; Luppi, E; Lupton, O; Machefert, F; Machikhiliyan, I V; Maciuc, F; Maev, O; Malde, S; Manca, G; Mancinelli, G; Maratas, J; Marconi, U; Marino, P; Märki, R; Marks, J; Martellotti, G; Martens, A; Martín Sánchez, A; Martinelli, M; Martinez Santos, D; Martins Tostes, D; Martynov, A; Massafferri, A; Matev, R; Mathe, Z; Matteuzzi, C; Maurice, E; Mazurov, A; McCann, M; McCarthy, J; McNab, A; McNulty, R; McSkelly, B; Meadows, B; Meier, F; Meissner, M; Merk, M; Milanes, D A; Minard, M-N; Molina Rodriguez, J; Monteil, S; Moran, D; Morawski, P; Mordà, A; Morello, M J

    2014-03-01

    Decays of B(s)(0) and B(0) mesons into J/ψ π+π-π+π- final states, produced in pp collisions at the LHC, are investigated using data corresponding to an integrated luminosity of 3 fb-1 collected with the LHCb detector. B(s)(0) → J/ψ f1(1285) decays are seen for the first time, and the branching fractions are measured. Using these rates, the f1(1285) mixing angle between strange and nonstrange components of its wave function in the qq structure model is determined to be ±(24.0-2.6-0.8+3.1+0.6)°. Implications on the possible tetraquark nature of the f1(1285) are discussed.

  15. CDK4, pRB and E2F1: connected to insulin

    Directory of Open Access Journals (Sweden)

    Blanchet Emilie

    2010-02-01

    Full Text Available Abstract Pancreatic β-cells are metabolic sensors involved in the control of glucose homeostasis. This particular cell type controls insulin secretion through a fine-tuned process, which dregulation have important pathological consequences, such as observed during type 2 diabetes. We recently implicated E2F1 in the control of glucose homeostasis. First we showed that E2f1-/- mice have decreased pancreatic size, as the result of impaired postnatal pancreatic growth. We observed in this study that E2F1 was highly expressed in non-proliferating pancreatic β-cells, suggesting that E2F1, besides the control of β-cell number could have a role in pancreatic β-cell function. We demonstrate in our recent study, both in vitro and in vivo that E2F1 directly regulates the expression of Kir6.2, a key component of the KATP channel involved in the regulation of glucose-induced insulin secretion in pancreatic β-cells. Expression of Kir6.2 is lost in pancreas of E2f1-/- mice, resulting in insulin secretion defects in these mice. Furthermore, we demonstrated by in tissue chromatin immunoprecipitation analysis that regulation of Kir6.2 expression by E2F1 follows the same regulatory pathway that the classical E2F1 target genes, implicating the participation of CDK4 and retinoblastoma protein. Moreover, in this context, E2F1 transcriptional activity is regulated by glucose and insulin through the CDK4-dependent inactivation of the pRB protein. In summary we provide evidence that the CDK4-pRB-E2F1 regulatory pathway is involved in glucose homeostasis. In our recent study we decipher a new function for these factors in the control of insulin secretion and open up new avenues for the treatment of metabolic diseases, in particular type 2 diabetes.

  16. Multicentric evaluation of a new assay for prothrombin fragment F1+2 determination.

    Science.gov (United States)

    Bruhn, H D; Conard, J; Mannucci, M; Monteagudo, J; Pelzer, H; Reverter, J C; Samama, M; Tripodi, A; Wagner, C

    1992-10-01

    A multicenter study of a recently developed ELISA for the determination of prothrombin fragment F1+2 was performed in order to evaluate analytical and clinical aspects. Mean intra-assay and inter-assay reproducibility were found to be 11.0 and 12.6%, respectively. The measuring range covered by the calibration curve reaches from 0.04 to 10.0 nM/l F1+2. Testing 133 healthy subjects a reference range of 0.37 to 1.11 nM/l F1+2 (2.5-97.5 percentile) with a median of 0.66 nM/l F1+2 was calculated. Minor difficulties with blood sampling (venous occlusion for 2 min) did not affect F1+2 plasma concentrations. Significantly increased F1+2 levels were measured in patients with leukemia (p < 0.0001), severe liver disease (p < 0.005) and after myocardial infarction (p < 0.01). Elevated F1+2 concentration before the beginning of heparin therapy (1.25 nM/l) decreased to 0.77 nM/l (p < 0.0001) after 1 day of therapy. For patients in the stable phase of oral anticoagulant therapy decreasing F1+2 concentrations were measured with increasing INR. F1+2 levels were already significantly reduced in patients with INR < 2.0 (0.56 nM/l; p = 0.0005). Thus F1+2 determination may be helpful in identifying activation processes as well as in monitoring anticoagulant therapy.

  17. Bio F1B hamster: a unique animal model with reduced lipoprotein lipase activity to investigate nutrient mediated regulation of lipoprotein metabolism

    Directory of Open Access Journals (Sweden)

    Cornish Marion L

    2007-12-01

    Full Text Available Abstract Background Bio F1B hamster is an inbred hybrid strain that is highly susceptible to diet-induced atherosclerosis. We previously reported that feeding a high fat fish oil diet to Bio F1B hamster caused severe hyperlipidaemia. In this study we compared the effects of various diets in the Bio F1B hamster and the Golden Syrian hamster, which is an outbred hamster strain to investigate whether genetic background plays an important role in dietary fat mediated regulation of lipoprotein metabolism. We further investigated the mechanisms behind diet-induced hyperlipidaemia in F1B hamster. Methods The Bio F1B and Golden Syrian hamsters, 8 weeks old, were fed high fat diets rich in either monounsaturated fatty acids, an n-6: n-3 ratio of 5 or a fish oil diet for 4 weeks. Animals were fasted overnight and blood and tissue samples were collected. Plasma was fractionated into various lipoprotein fractions and assayed for triacylglycerol and cholesterol concentrations. Plasma lipoprotein lipase activity was measured using radioisotope method. Microsomal triglyceride transfer protein activity was measured in the liver and intestine. Plasma apolipoproteinB48, -B100 and apolipoprotein E was measured using Western blots. Two-way ANOVA was used to determine the effect of diet type and animal strain. Results The fish oil fed F1B hamsters showed milky plasma after a 14-hour fast. Fish oil feeding caused accumulation of apolipoproteinB48 containing lipoprotein particles suggesting hindrance of triglyceride-rich lipoprotein clearance. There was no significant effect of diet or strain on hepatic or intestinal microsomal triglyceride transfer protein activity indicating that hyperlipidaemia is not due to an increase in the assembly or secretion of lipoprotein particles. F1B hamsters showed significantly reduced levels of lipoprotein lipase activity, which was inhibited by fish oil feeding. Conclusion Evidence is presented for the first time that alterations in

  18. Rotation of subunits during catalysis by Escherichia coli F1-ATPase.

    Science.gov (United States)

    Duncan, T M; Bulygin, V V; Zhou, Y; Hutcheon, M L; Cross, R L

    1995-11-21

    During oxidative and photo-phosphorylation, F0F1-ATP synthases couple the movement of protons down an electrochemical gradient to the synthesis of ATP. One proposed mechanistic feature that has remained speculative is that this coupling process requires the rotation of subunits within F0F1. Guided by a recent, high-resolution structure for bovine F1 [Abrahams, J. P., Leslie, A. G., Lutter, R. & Walker, J. E. (1994) Nature (London) 370, 621-628], we have developed a critical test for rotation of the central gamma subunit relative to the three catalytic beta subunits in soluble F1 from Escherichia coli. In the bovine F1 structure, a specific point of contact between the gamma subunit and one of the three catalytic beta subunits includes positioning of the homolog of E. coli gamma-subunit C87 (gamma C87) close to the beta-subunit 380DELSEED386 sequence. A beta D380C mutation allowed us to induce formation of a specific disulfide bond between beta and gamma C87 in soluble E. coli F1. Formation of the crosslink inactivated beta D380C-F1, and reduction restored full activity. Using a dissociation/reassembly approach with crosslinked beta D380C-F1, we incorporated radiolabeled beta subunits into the two noncrosslinked beta-subunit positions of F1. After reduction of the initial nonradioactive beta-gamma crosslink, only exposure to conditions for catalytic turnover results in similar reactivities of unlabeled and radiolabeled beta subunits with gamma C87 upon reoxidation. The results demonstrate that gamma subunit rotates relative to the beta subunits during catalysis.

  19. Absence of pRb facilitates E2F1-induced apoptosis in breast cancer cells.

    Science.gov (United States)

    Sun, Baohua; Wingate, Hannah; Swisher, Stephen G; Keyomarsi, Khandan; Hunt, Kelly K

    2010-03-15

    The transcription factor E2F1 is known for its interaction with pRb, controlling cell proliferation; however, E2F1 also has a pivotal role in regulating apoptosis.  The relationship between pRb and E2F1 balances cell proliferation and apoptosis giving pRb tumor suppressive properties. The intricacies of the pRb/E2F1 relationship and thus the regulation of cell fate is cell context dependent. To explore the role of pRb in the E2F1-induced apoptosis of human breast cancer cells, we examined cell growth and apoptosis induction in isogenic cell systems of immortalized breast epithelial cells lacking either pRb (76NE7) or p53 (76NE6). We found that E2F1 caused accumulation of cells in G2 and S phases of the cell cycle along with apoptosis in 76NE7 but not 76NE6 cells.  Variants of 76NE6 cells with functional p53 did not rescue the apoptotic response in these cells, whereas knocking down pRb resulted in significant E2F1-induced apoptosis. We also determined that the effect of E2F1 overexpression in two breast cancer cell lines, MDA-MB-436 and MDA-MB-468, which lack pRb and functional p53, was accumulation of cells in G2/S phase and apoptosis. However, E2F did not cause apotosis  in MCF-7 cells which harbor a functional pRb. Therefore, we conclude that in the absence of Rb, E2F1 overexpression results in apoptosis, not proliferation, and that this effect is independent of p53.

  20. 17 CFR 270.18f-1 - Exemption from certain requirements of section 18(f)(1) (of the Act) for registered open-end...

    Science.gov (United States)

    2010-04-01

    ... requirements of section 18(f)(1) (of the Act) for registered open-end investment companies which have the right... which have the right to redeem in kind. (a) A registered open-end investment company which has the right... to pay in cash all requests for redemption by any shareholder of record, limited in amount...

  1. Remaining Sites Verification Package for the 1607-F1 Sanitary Sewer System (124-F-1) and the 100-F-26:8 (1607-F1) Sanitary Sewer Pipelines Waste Sites, Waste Site Reclassification Form 2004-130

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Dittmer

    2008-03-14

    The 1607-F1 Sanitary Sewer System (124-F-1), consisted of a septic tank, drain field, and associated pipelines that received sanitary waste water from the 1701-F Gatehouse, 1709-F Fire Station, and the 1720-F Administrative Office via the 100-F-26:8 pipelines. The septic tank required remedial action based on confirmatory sampling. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling show that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also demonstrate that residual contaminant concentrations are protective of groundwater and the Columbia River.

  2. Remaining Sites Verification Package for the 1607-F1 Sanitary Sewer System (124-F-1) and the 100-F-26:8 (1607-F1) Sanitary Sewer Pipelines Waste Sites, Waste Site Reclassification Form 2004-130

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Dittmer

    2008-03-14

    The 1607-F1 Sanitary Sewer System (124-F-1), consisted of a septic tank, drain field, and associated pipelines that received sanitary waste water from the 1701-F Gatehouse, 1709-F Fire Station, and the 1720-F Administrative Office via the 100-F-26:8 pipelines. The septic tank required remedial action based on confirmatory sampling. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling show that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also demonstrate that residual contaminant concentrations are protective of groundwater and the Columbia River.

  3. Rotation of subunits during catalysis by Escherichia coli F1-ATPase.

    OpenAIRE

    1995-01-01

    During oxidative and photo-phosphorylation, F0F1-ATP synthases couple the movement of protons down an electrochemical gradient to the synthesis of ATP. One proposed mechanistic feature that has remained speculative is that this coupling process requires the rotation of subunits within F0F1. Guided by a recent, high-resolution structure for bovine F1 [Abrahams, J. P., Leslie, A. G., Lutter, R. & Walker, J. E. (1994) Nature (London) 370, 621-628], we have developed a critical test for rotation ...

  4. Optical pumping effect in absorption imaging of F=1 atomic gases

    CERN Document Server

    Kim, Sooshin; Noh, Heung-Ryoul; Shin, Y

    2016-01-01

    We report our study of the optical pumping effect in absorption imaging of $^{23}$Na atoms in the $F=1$ hyperfine spin states. Solving a set of rate equations for the spin populations under a probe beam, we obtain an analytic expression for the optical signal of the $F=1$ absorption imaging. Furthermore, we verify the result by measuring the absorption spectra of $^{23}$Na Bose-Einstein condensates prepared in various spin states with different probe beam pulse durations. The analytic result can be used in quantitative analysis of $F=1$ spinor condensate imaging and readily applied to other alkali atoms with $I=3/2$ nuclear spin such as $^{87}$Rb.

  5. ESR-spektroskopische Untersuchungen der F0F1-ATP-Synthase aus Escherichia coli

    OpenAIRE

    Motz, Christian

    1999-01-01

    Die FoF1-ATP-Synthase katalysiert die Synthese von ATP aus ADP und Pi bei der oxidativen bzw. Photophosphorylierung. Der ATP-Synthase-Komplex läßt sich in zwei funktionelle Einheiten unterteilen: Fo ist ein integraler Membranproteinkomplex, der den Protonenkanal bildet. F1 hingegen ist ein wasserlöslicher Proteinkomplex, der die Nukleotidbindungsstellen trägt. Die ATP-Synthase aus Escherichia coli hat die Zusammensetzung alpha3beta3gamma delta epsilon für die F1 und ab2c9-12 für den Fo-Teil. ...

  6. Characterization of an F1 deletion mutant of Yersinia pestis CO92, pathogenic role of F1 antigen in bubonic and pneumonic plague, and evaluation of sensitivity and specificity of F1 antigen capture-based dipsticks.

    Science.gov (United States)

    Sha, Jian; Endsley, Janice J; Kirtley, Michelle L; Foltz, Sheri M; Huante, Matthew B; Erova, Tatiana E; Kozlova, Elena V; Popov, Vsevolod L; Yeager, Linsey A; Zudina, Irina V; Motin, Vladimir L; Peterson, Johnny W; DeBord, Kristin L; Chopra, Ashok K

    2011-05-01

    We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipsticks, an in-frame F1-deficient mutant of CO92 (Δcaf) was generated by homologous recombination and used as a negative control. Based on genetic, antigenic/immunologic, and electron microscopic analyses, the Δcaf mutant was devoid of a capsule. The growth rate of the Δcaf mutant generally was similar to that of the wild-type (WT) bacterium at both 26 and 37 °C, although the mutant's growth dropped slightly during the late phase at 37 °C. The Δcaf mutant was as virulent as WT CO92 in the pneumonic plague mouse model; however, it was attenuated in developing bubonic plague. Both dipsticks had similar sensitivities, requiring a minimum of 0.5 μg/ml of purified F1 antigen or 1 × 10(5) to 5 × 10(5) CFU/ml of WT CO92 for positive results, while the blood samples were negative for up to 1 × 10(8) CFU/ml of the Δcaf mutant. Our studies demonstrated the diagnostic potential of two plague dipsticks in detecting capsular-positive strains of Y. pestis in bubonic and pneumonic plague.

  7. Fine mapping of locus S-b for F1 pollen sterility in rice (Oryza sativa L.)

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Hybrid sterility is the main barrier in utilizing the heterosis of subspecies in rice. A knowledge of the underlying molecular mechanism of the hybrid sterility will be useful for overcoming the barrier. In this research, the F1 pollen sterility locus, S-b, was mapped between SSR markers PSM8 and PSM202. To fine map the locus, one F2 mapping population of 3910 plants was developed using the near-isogenic lines of the locus. Ninety-seven recombinants between two markers were selected. Moreover, a series of markers, including two SSR markers, two InDel markers and four CAPS markers, were developed on the region. Linkage analysis showed that marker W4 was co-segregated with locus S-b, while makers A8 and A14 were located on the two sides of the locus with a distance of 0.026 and 0.038 cM, respectively. The markers were then integrated with the sequences of the clones of the region. Results showed that all the polymorphic markers were anchored on the three end-to-end jointed clones AC093089, AC079021 and AC134931. According to the physical information of the markers, locus S-b was finally delimited to a region of 27 kb between A8 and A14. Seven ORFs were identified on the region based on the annotation results of RiceGAAS system. These results laid the foundation for further cloning the gene.

  8. Cloning and Identification of Porcine HSPC117 Gene Differentially Expressed in F1 Crossbreds and Their Parents

    Institute of Scientific and Technical Information of China (English)

    XIE Hong-tao; LEI Ming-gang; XIONG Yuan-zhu; DENG Chang-yan; JIANG Si-wen; LI Feng-e; ZUO Bo; XU De-quan

    2007-01-01

    To investigate the molecular basis of porcine heterosis, suppression subtractive hybridization (SSH) was performed to detect the differences in gene expression between porcine longissimus dorsi of Meishan × Large White (MS × LW) F1hybrids and their parents Meishan pigs. An expression sequence tag (EST) differentially expressed was found, designated as ML556, which was homologous to a hypothetical protein HSPC117, from human hematopoietic stem/progenitor cells(HSPCs), and the full-length cDNA of porcine HSPC117 was cloned using rapid amplification of cDNA ends (RACE)method. Translation of the mRNA transcript revealed an open reading frame (ORF) of 505 amino acid residues encoding a peroxisomal targeting signal (PTS) with theoretical molecular weight of 55 kDa. Alignment analysis revealed that the deduced protein sequence exhibit 98, 98, 98, 97, and 97% identity with that of cattle, human, dog, rat, and mouse,respectively. The tissue expression analysis indicated that the porcine HSPC117 gene is highly expressed in muscle,spleen, lung, kidney, uterus, ovary and testis, moderately expressed in fat, heart, and liver, and not expressed in stomach and small intestine. The possible role of porcine HSPC117 and its relationship with porcine heterosis were discussed.

  9. Arabidopsis RabF1 (ARA6) Is Involved in Salt Stress and Dark-Induced Senescence (DIS)

    Science.gov (United States)

    Yin, Congfei; Karim, Sazzad; Zhang, Hongsheng; Aronsson, Henrik

    2017-01-01

    Arabidopsis small GTPase RabF1 (ARA6) functions in endosomal vesicle transport and may play a crucial role in recycling and degradation of molecules, thus involved in stress responses. Here we have reported that complementary overexpression lines RabF1OE (overexpression), GTPase mutants RabF1Q93L (constitutively active) and RabF1S47N (dominant negative) lines show longer root growth than wild-type, rabF1 knockout and N-myristoylation deletion (Δ1−29, N-terminus) complementary overexpression mutant plants under salt induced stress, which indicates that N-myristoylation of RabF1 is indispensable for salt tolerance. Moreover, RabF1 is highly expressed during senescence and RabF1OE lines were more tolerant of dark-induced senescence (DIS) than wild-type and rabF1. PMID:28157156

  10. Repression of androgen receptor transcription through the E2F1/DNMT1 axis.

    Directory of Open Access Journals (Sweden)

    Conrad David Valdez

    Full Text Available Although androgen receptor (AR function has been extensively studied, regulation of the AR gene itself has been much less characterized. In this study, we observed a dramatic reduction in the expression of androgen receptor mRNA and protein in hyperproliferative prostate epithelium of keratin 5 promoter driven E2F1 transgenic mice. To confirm an inhibitory function for E2F1 on AR transcription, we showed that E2F1 inhibited the transcription of endogenous AR mRNA, subsequent AR protein, and AR promoter activity in both human and mouse epithelial cells. E2F1 also inhibited androgen-stimulated activation of two AR target gene promoters. To elucidate the molecular mechanism of E2F-mediated inhibition of AR, we evaluated the effects of two functional E2F1 mutants on AR promoter activity and found that the transactivation domain appears to mediate E2F1 repression of the AR promoter. Because DNMT1 is a functional intermediate of E2F1 we examined DNMT1 function in AR repression. Repression of endogenous AR in normal human prostate epithelial cells was relieved by DNMT1 shRNA knock down. DNMT1 was shown to be physically associated within the AR minimal promoter located 22 bps from the transcription start site; however, methylation remained unchanged at the promoter regardless of DNMT1 expression. Taken together, our results suggest that DNMT1 operates either as a functional intermediary or in cooperation with E2F1 inhibiting AR gene expression in a methylation independent manner.

  11. 26 CFR 31.3121(f)-1 - American vessel and aircraft.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 15 2010-04-01 2010-04-01 false American vessel and aircraft. 31.3121(f)-1... § 31.3121(f)-1 American vessel and aircraft. (a) The term “American vessel” means any vessel which is...”, see § 31.3121 (e)-1.) (b) The term “American aircraft” means any aircraft registered under the laws of...

  12. 26 CFR 1.669(f)-1A - Character of capital gain.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Character of capital gain. 1.669(f)-1A Section 1... Before January 1, 1969 § 1.669(f)-1A Character of capital gain. Amounts distributed as a capital gain... the gain had with respect to the trust. Thus, a capital gain that was taxed to the trust as a...

  13. 新西兰Hulme F1公路超级跑车

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    新西兰Kiwi公司开发的 F1公路超级跑车将于今年登场。该车以1967年F1大奖赛冠军得主新西兰人Denny Hulme名字命名为Hulme。它用了两年时间进行设计和打造。

  14. NR2F1 controls tumor cell dormancy via SOX9 and RARβ driven quiescence programs

    Science.gov (United States)

    Sosa, Maria Soledad; Parikh, Falguni; Maia, Alexandre Gaspar; Estrada, Yeriel; Bosch, Almudena; Bragado, Paloma; Ekpin, Esther; George, Ajish; Zheng, Yang; Lam, Hung-Ming; Morrissey, Colm; Chung, Chi-Yeh; Farias, Eduardo F.; Bernstein, Emily; Aguirre-Ghiso, Julio A.

    2014-01-01

    Metastases can originate from disseminated tumor cells (DTCs), which may be dormant for years before reactivation. Here we find that the orphan nuclear receptor NR2F1 is epigenetically upregulated in experimental HNSCC dormancy models and in DTCs from prostate cancer patients carrying dormant disease for 7–18 years. NR2F1-dependent dormancy is recapitulated by a co-treatment with the DNA demethylating agent 5-Aza-C and retinoic acid across various cancer types. NR2F1-induced quiescence is dependent on SOX9, RARβ and CDK inhibitors. Intriguingly, NR2F1 induces global chromatin repression and the pluripotency gene NANOG, which contributes to dormancy of DTCs in the bone marrow. When NR2F1 is blocked in vivo, growth arrest or survival of dormant DTCs is interrupted in different organs. We conclude that NR2F1 is a critical node in dormancy induction and maintenance by integrating epigenetic programs of quiescence and survival in DTCs. PMID:25636082

  15. E2F1-mediated human POMC expression in ectopic Cushing's syndrome.

    Science.gov (United States)

    Araki, Takako; Liu, Ning-Ai; Tone, Yukiko; Cuevas-Ramos, Daniel; Heltsley, Roy; Tone, Masahide; Melmed, Shlomo

    2016-11-01

    Cushing's syndrome is caused by excessive adrenocorticotropic hormone (ACTH) secretion derived from pituitary corticotroph tumors (Cushing disease) or from non-pituitary tumors (ectopic Cushing's syndrome). Hypercortisolemic features of ectopic Cushing's syndrome are severe, and no definitive treatment for paraneoplastic ACTH excess is available. We aimed to identify subcellular therapeutic targets by elucidating transcriptional regulation of the human ACTH precursor POMC (proopiomelanocortin) and ACTH production in non-pituitary tumor cells and in cell lines derived from patients with ectopic Cushing's syndrome. We show that ectopic hPOMC transcription proceeds independently of pituitary-specific Tpit/Pitx1 and demonstrate a novel E2F1-mediated transcriptional mechanism regulating hPOMC We identify an E2F1 cluster binding to the proximal hPOMC promoter region (-42 to +68), with DNA-binding activity determined by the phosphorylation at Ser-337. hPOMC mRNA expression in cancer cells was upregulated (up to 40-fold) by the co-expression of E2F1 and its heterodimer partner DP1. Direct and indirect inhibitors of E2F1 activity suppressed hPOMC gene expression and ACTH by modifying E2F1 DNA-binding activity in ectopic Cushing's cell lines and primary tumor cells, and also suppressed paraneoplastic ACTH and cortisol levels in xenografted mice. E2F1-mediated hPOMC transcription is a potential target for suppressing ACTH production in ectopic Cushing's syndrome. © 2016 Society for Endocrinology.

  16. The fertility of the hybrid lineage derived from female Megalobrama amblycephala × male Culter alburnus.

    Science.gov (United States)

    Xiao, Jun; Kang, Xuewei; Xie, Lihua; Qin, Qinbo; He, Zhoulin; Hu, Fangzhou; Zhang, Chun; Zhao, Rurong; Wang, Jun; Luo, Kaikun; Liu, Yun; Liu, Shaojun

    2014-12-10

    Distant hybridization can combine together the genomes of different species, which leads to changes of the offspring in phenotypes and genotypes. In this study, we successfully establish a fertile hybrid lineage by intergeneric hybridization of female blunt snout bream (BSB, Megalobrama amblycephala) × male topmouth culter (TC, Culter alburnus) and investigate some important biological traits of this lineage including the morphological traits, chromosomal number, karyotype, DNA content, gonadal development, egg and milt yield, sperm shape and density, fertilization rate and early survival rate. The results show that: (1) the diploid and triploid hybrids coexist in F1 and only diploid hybrids are found in F2, in which the diploid hybrids of F1 and F2 possess 48 chromosomes with one chromosome set of BSB and one chromosome set of TC, and the triploid hybrids of F1 possess 72 chromosomes with two chromosome sets of BSB and one chromosome set of TC. (2) All the tested males and females of the diploid F1 and F2 hybrids have the normal gonadal development and produce mature sperm and egg, respectively, which are fertilized with each other to form F2 and F3 hybrids, respectively, and finally form a diploid hybrid lineage (F1-F3). (3) The good fertility of the F1 and F2 hybrids of female BSB × male TC potentially provides reproductive base to make the hybrid lineage propagate from one generation to another. The formation of the hybrid lineage (F1-F3) also provides an ideal model to research the reproductive rules of distant hybrid progeny.

  17. Ants exhibit asymmetric hybridization in a mosaic hybrid zone.

    Science.gov (United States)

    Purcell, Jessica; Zahnd, Sacha; Athanasiades, Anouk; Türler, Rebecca; Chapuisat, Michel; Brelsford, Alan

    2016-10-01

    Research on hybridization between species provides unparalleled insights into the pre- and postzygotic isolating mechanisms that drive speciation. In social organisms, colony-level incompatibilities may provide additional reproductive barriers not present in solitary species, and hybrid zones offer an opportunity to identify these barriers. Here, we use genotyping-by-sequencing to sequence hundreds of markers in a hybrid zone between two socially polymorphic ant species, Formica selysi and Formica cinerea. We characterize the zone, determine the frequency of hybrid workers, infer whether hybrid queens or males are produced and investigate whether hybridization is influenced by colony social organization. We also compare cuticular hydrocarbon profiles and aggression levels between the two species. The hybrid zone exhibits a mosaic structure. The asymmetric distribution of hybrids skewed towards F. cinerea suggests a pattern of unidirectional nuclear gene flow from F. selysi into F. cinerea. The occurrence of backcrossed individuals indicates that hybrid queens and/or males are fertile, and the presence of the F. cinerea mitochondrial haplotype in 97% of hybrids shows that successful F1 hybrids will generally have F. cinerea mothers and F. selysi fathers. We found no evidence that social organization contributes to speciation, because hybrids occur in both single-queen and multiple-queen colonies. Strongly differentiated cuticular hydrocarbon profiles and heightened interspecific aggression further reveal that species recognition cues are both present and perceived. The discovery of fertile hybrids and asymmetrical gene flow is unusual in ants, and this hybrid zone will therefore provide an ideal system with which to investigate speciation in social insects.

  18. Phylodynamics of HIV-1 subtype F1 in Angola, Brazil and Romania.

    Science.gov (United States)

    Bello, Gonzalo; Afonso, Joana Morais; Morgado, Mariza G

    2012-07-01

    The HIV-1 subtype F1 is exceptionally prevalent in Angola, Brazil and Romania. The epidemiological context in which the spread of HIV occurred was highly variable from one country to another, mainly due to the existence of a long-term civil war in Angola and the contamination of a large number of children in Romania. Here we apply phylogenetic and Bayesian coalescent-based methods to reconstruct the phylodynamic patterns of HIV-1 subtype F1 in such different epidemiological settings. The phylogenetic analyses of HIV-1 subtype F1 pol sequences sampled worldwide confirmed that most sequences from Angola, Brazil and Romania segregated in country-specific monophyletic groups, while most subtype F1 sequences from Romanian children branched as a monophyletic sub-cluster (Romania-CH) nested within sequences from adults. The inferred time of the most recent common ancestor of the different subtype F1 clades were as follow: Angola=1983 (1978-1989), Brazil=1977 (1972-1981), Romania adults=1980 (1973-1987), and Romania-CH=1985 (1978-1989). All subtype F1 clades showed a demographic history best explained by a model of logistic population growth. Although the expansion phase of subtype F1 epidemic in Angola (mid 1980s to early 2000s) overlaps with the civil war period (1975-2002), the mean estimated growth rate of the Angolan F1 clade (0.49 year(-1)) was not exceptionally high, but quite similar to that estimated for the Brazilian (0.69 year(-1)) and Romanian adult (0.36 year(-1)) subtype F1 clades. The Romania-CH subtype F1 lineage, by contrast, displayed a short and explosive dissemination phase, with a median growth rate (2.47 year(-1)) much higher than that estimated for adult populations. This result supports the idea that the AIDS epidemic that affected the Romanian children was mainly caused by the spread of the HIV through highly efficient parenteral transmission networks, unlike adult populations where HIV is predominantly transmitted through sexual route.

  19. 原鸡与不同品种家鸡杂交F1代生长发育研究%Study on the Growth and Development of F1 Generation between Gallus gallus and Different Varieties of Gallus domesticus

    Institute of Scientific and Technical Information of China (English)

    马先才; 彭章华; 雷胜辉; 周杰珑

    2012-01-01

    [Objective] The research aimed to discuss the growth and development laws of hybnd offspring from Gallus gallus and different local chicken varieties. [Method] Gallus gallus spadiceus (male) and different Gallus domesticus varieties (female) (Chuxiong Ma Chicken, Chahua Chicken, Green-ear Black-bone Chicken,Gallus gallus) were used as study objects to determine the growth and development indices of 0-22 week-old chicken. Using three typical nonlinear growth curve models (Logistic, Gompertz and Bertalanffy), the growth models of hybrid F1 generation were fit. Meanwhile, the optimal regression equation was established by using stepwise regression method. [Result] The effect degree or indices of the body traits of hybrid F1 generation between Gallus gallus and different local chicken varieties on the body weight had some differences. The fitting degree of the regression equation of Logistic and Gompertz models were both more than 0. 99, which better fit the growth and development laws of F1 hybrid generation of Gallus gallus. [Conclusion] This research provided scientific basis and reference for the preservation, utilization of genetic resources of Gallus gallus, and the breeding and improvement of local domestic chicken varieties.%[目的]探讨原鸡与不同地方品种家鸡杂交后代的生长发育规律.[方法]以原鸡(♂)与不同地方鸡种(♀)(楚雄麻鸡、茶花鸡、绿耳乌骨鸡、原鸡)杂交组合F1代(♂)为研究对象,测定其0~22周龄的生长发育指标.运用Logistic、Gompertz和Bertalanffy3种典型的非线性生长曲线模型分别对其进行拟合分析,同时利用逐步回归法建立最优回归方程.[结果]原鸡与不同家鸡杂交F1代(♂)体尺性状对体重影响程度或指标存在一定差异.Logistic和Gompertz模型回归方程的拟合度都达到0.99以上,很好地模拟了原鸡杂交代F1的生长发育规律.[结论]该研究为原鸡遗传资源的保存、利用和地方家

  20. Remaining Sites Verification Package for the 1607-F1 Sanitary Sewer System (124-F-1) and the 100-F-26:8 (1607-F1) Sanitary Sewer Pipelines Waste Sites, Waste Site Reclassification Form 2005-004

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Dittmer

    2008-03-14

    The 100-F-26:8 waste site consisted of the underground pipelines that conveyed sanitary waste water from the 1701-F Gatehouse, 1709-F Fire Station, and the 1720-F Administrative Office to the 1607-F1 septic tank. The site has been remediated and presently exists as an open excavation. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling demonstrated that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also showed that residual contaminant concentrations are protective of groundwater and the Columbia River.

  1. Remaining Sites Verification Package for the 1607-F1 Sanitary Sewer System (124-F-1) and the 100-F-26:8 (1607-F1) Sanitary Sewer Pipelines Waste Sites, Waste Site Reclassification Form 2005-004

    Energy Technology Data Exchange (ETDEWEB)

    L. M. Dittmer

    2008-03-14

    The 100-F-26:8 waste site consisted of the underground pipelines that conveyed sanitary waste water from the 1701-F Gatehouse, 1709-F Fire Station, and the 1720-F Administrative Office to the 1607-F1 septic tank. The site has been remediated and presently exists as an open excavation. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling demonstrated that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also showed that residual contaminant concentrations are protective of groundwater and the Columbia River.

  2. Development of Yersinia pestis F1 antigen-loaded microspheres vaccine against plague

    Directory of Open Access Journals (Sweden)

    Huang SS

    2014-02-01

    Full Text Available Shih-shiung Huang,1 I-Hsun Li,2,3 Po-da Hong,1 Ming-kung Yeh1,2,41Biomedical Engineering Program, Graduate Institute of Engineering, Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, Republic of China; 2School of Pharmacy, 3Department of Pharmacy Practice, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China; 4Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan, Republic of ChinaAbstract: Yersinia pestis F1 antigen-loaded poly(DL-lactide-co-glycolide/polyethylene glycol (PEG (PLGA/PEG microspheres were produced using a water-in-oil-in-water emulsion/solvent extraction technique and assayed for their percent yield, entrapment efficiency, surface morphology, particle size, zeta potential, in vitro release properties, and in vivo animal protect efficacy. The Y. pestis F1 antigen-loaded microspheres (mean particle size 3.8 µm exhibited a high loading capacity (4.5% w/w, yield (85.2%, and entrapment efficiency (38.1%, and presented a controlled in vitro release profile with a low initial burst (18.5%, then continued to release Y. pestis F1 antigen over 70 days. The distribution (% of Y. pestis F1 on the microspheres surface, outer layer, and core was 3.1%, 28.9%, and 60.7%, respectively. A steady release rate was noticed to be 0.55 µg Y. pestis F1 antigen/mg microspheres/day of Y. pestis F1 antigen release maintained for 42 days. The cumulative release amount at the 1st, 28th, and 42nd days was 8.2, 26.7, and 31.0 µg Y. pestis F1 antigen/mg microspheres, respectively. The 100 times median lethal dose 50% (LD50 of Y. pestis Yokohama-R strain by intraperitoneal injection challenge in mice test, in which mice received one dose of 40 µg F1 antigen content of PLGA/PEG microspheres, F1 antigen in Al(OH3, and in comparison with F1 antigen in Al(OH3 vaccine in two doses, was evaluated after given by subcutaneous

  3. Evaluation of the recombinant protein TpF1 of Treponema pallidum for serodiagnosis of syphilis.

    Science.gov (United States)

    Jiang, Chuanhao; Zhao, Feijun; Xiao, Jinhong; Zeng, Tiebing; Yu, Jian; Ma, Xiaohua; Wu, Haiying; Wu, Yimou

    2013-10-01

    Syphilis is a chronic infection caused by Treponema pallidum subsp. pallidum, and diagnosis with sensitive and specific methods is a challenging process that is important for its prevention and treatment. In the present study, we established a recombinant protein TpF1-based indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and a Western blot assay for human and rabbit sera. The 20-kDa recombinant protein TpF1 was detected by Western blotting performed with sera from rabbits immunized with recombinant TpF1 and infected with the T. pallidum Nichols strain and T. pallidum clinical isolates but was not detected by Western blotting with sera from uninfected rabbits. The sensitivity of the recombinant protein was determined by screening sera from individuals with primary, secondary, latent, and congenital syphilis (n = 82). The specificity of the recombinant protein was determined by screening sera from uninfected controls (n = 30) and individuals with potentially cross-reactive infections, including Lyme disease (n = 30) and leptospirosis (n = 5). The sensitivities of TpF1-based ELISAs were 93.3%, 100%, 100%, and 100% for primary, secondary, latent, and congenital syphilis, respectively, and the specificities were all 100% for sera from uninfected controls and individuals with potentially cross-reactive infections. In Western blot assays, the sensitivities and specificities of TpF1 for human sera were all 100%. The reactivities of TpF1 with syphilitic sera were proportional to the titers of the T. pallidum particle agglutination (TPPA) assay. These data indicate that the recombinant protein TpF1 is a highly immunogenic protein in human and rabbit infections and a promising marker for the screening of syphilis.

  4. Cooperative activation of tissue-specific genes by pRB and E2F1.

    Science.gov (United States)

    Flowers, Stephen; Xu, Fuhua; Moran, Elizabeth

    2013-04-01

    The retinoblastoma tumor suppressor protein pRB is conventionally regarded as an inhibitor of the E2F family of transcription factors. Conversely, pRB is also recognized as an activator of tissue-specific gene expression along various lineages including osteoblastogenesis. During osteoblast differentiation, pRB directly targets Alpl and Bglap, which encode the major markers of osteogenesis alkaline phosphatase and osteocalcin. Surprisingly, p130 and repressor E2Fs were recently found to cooccupy and repress Alpl and Bglap in proliferating osteoblast precursors before differentiation. This raises the further question of whether these genes convert to E2F activation targets when differentiation begins, which would constitute a remarkable situation wherein pRB and E2F would be cotargeting genes for activation. Chromatin immunoprecipitation analysis in an osteoblast differentiation model shows that Alpl and Bglap are indeed targeted by an activator E2F, i.e., is E2F1. Promoter occupation of Alpl and Bglap by E2F1 occurs specifically during activation, and depletion of E2F1 severely impairs their induction. Mechanistically, promoter occupation by E2F1 and pRB is mutually dependent, and without this cooperative effect, activation steps previously shown to be dependent on pRB, including recruitment of RNA polymerase II, are impaired. Myocyte- and adipocyte-specific genes are also cotargeted by E2F1 and pRB during differentiation along their respective lineages. The finding that pRB and E2F1 cooperate to activate expression of tissue-specific genes is a paradigm distinct from the classical concept of pRB as an inhibitor of E2F1, but is consistent with the observed roles of these proteins in physiological models.

  5. Scavenger receptors mediate the role of SUMO and Ftz-f1 in Drosophila steroidogenesis.

    Directory of Open Access Journals (Sweden)

    Ana Talamillo

    2013-04-01

    Full Text Available SUMOylation participates in ecdysteroid biosynthesis at the onset of metamorphosis in Drosophila melanogaster. Silencing the Drosophila SUMO homologue smt3 in the prothoracic gland leads to reduced lipid content, low ecdysone titers, and a block in the larval-pupal transition. Here we show that the SR-BI family of Scavenger Receptors mediates SUMO functions. Reduced levels of Snmp1 compromise lipid uptake in the prothoracic gland. In addition, overexpression of Snmp1 is able to recover lipid droplet levels in the smt3 knockdown prothoracic gland cells. Snmp1 expression depends on Ftz-f1 (an NR5A-type orphan nuclear receptor, the expression of which, in turn, depends on SUMO. Furthermore, we show by in vitro and in vivo experiments that Ftz-f1 is SUMOylated. RNAi-mediated knockdown of ftz-f1 phenocopies that of smt3 at the larval to pupal transition, thus Ftz-f1 is an interesting candidate to mediate some of the functions of SUMO at the onset of metamorphosis. Additionally, we demonstrate that the role of SUMOylation, Ftz-f1, and the Scavenger Receptors in lipid capture and mobilization is conserved in other steroidogenic tissues such as the follicle cells of the ovary. smt3 knockdown, as well as ftz-f1 or Scavenger knockdown, depleted the lipid content of the follicle cells, which could be rescued by Snmp1 overexpression. Therefore, our data provide new insights into the regulation of metamorphosis via lipid homeostasis, showing that Drosophila Smt3, Ftz-f1, and SR-BIs are part of a general mechanism for uptake of lipids such as cholesterol, required during development in steroidogenic tissues.

  6. Molecular cloning of chicken FTZ-F1-related orphan receptors.

    Science.gov (United States)

    Kudo, T; Sutou, S

    1997-09-15

    FTZ-F1 is a member of the orphan nuclear receptors, which belongs to the steroid hormone receptor superfamily, and plays a role in the blastoderm and nervous system development in Drosophila. Recently, several FTZ-F1 family genes have been cloned in several species. SF-1/Ad4BPs have been identified as master regulators controlling steroidogenic P-450 genes in mammals and are considered to be the mammalian homologues of FTZ-F1. Moreover, SF-1/Ad4BP plays a critical role in the sexual differentiation of gonads in mammals. In vertebrates, except for mammals, the functional homologue of SF-1/Ad4BP has not been identified before. Herein, we cloned two chicken cDNAs (OR2.0 and OR2.1), which encode putative FTZ-F1 family receptors, by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). OR2.1 consists of 3255 bp, is expressed in the adrenal glands and gonads, and is considered to be the chicken counterpart of mammalian SF-1/Ad4BP. However, OR2.0 consists of 2945 bp, is expressed in the livers and the adrenal glands, and is considered to be the chicken counterpart of mouse LRH-1, which is a member of the FTZ-F1 family in mammals.

  7. Elasticity, friction, and pathway of γ-subunit rotation in FoF1-ATP synthase.

    Science.gov (United States)

    Okazaki, Kei-ichi; Hummer, Gerhard

    2015-08-25

    We combine molecular simulations and mechanical modeling to explore the mechanism of energy conversion in the coupled rotary motors of FoF1-ATP synthase. A torsional viscoelastic model with frictional dissipation quantitatively reproduces the dynamics and energetics seen in atomistic molecular dynamics simulations of torque-driven γ-subunit rotation in the F1-ATPase rotary motor. The torsional elastic coefficients determined from the simulations agree with results from independent single-molecule experiments probing different segments of the γ-subunit, which resolves a long-lasting controversy. At steady rotational speeds of ∼ 1 kHz corresponding to experimental turnover, the calculated frictional dissipation of less than k(B)T per rotation is consistent with the high thermodynamic efficiency of the fully reversible motor. Without load, the maximum rotational speed during transitions between dwells is reached at ∼ 1 MHz. Energetic constraints dictate a unique pathway for the coupled rotations of the Fo and F1 rotary motors in ATP synthase, and explain the need for the finer stepping of the F1 motor in the mammalian system, as seen in recent experiments. Compensating for incommensurate eightfold and threefold rotational symmetries in Fo and F1, respectively, a significant fraction of the external mechanical work is transiently stored as elastic energy in the γ-subunit. The general framework developed here should be applicable to other molecular machines.

  8. Induction of DNA synthesis and apoptosis are separable functions of E2F-1

    DEFF Research Database (Denmark)

    Phillips, A C; Bates, S; Ryan, K M;

    1997-01-01

    The family of E2F transcription factors have an essential role in mediating cell cycle progression, and recently, one of the E2F protein family, E2F-1, has been shown to participate in the induction of apoptosis. Cooperation between E2F and the p53 tumor suppressor protein in this apoptotic...... response had led to the suggestion that cell cycle progression induced by E2F-1 expression provides an apoptotic signal when placed in conflict with an arrest to cell cycle progression, such as provided by p53. We show here that although apoptosis is clearly enhanced by p53, E2F-1 can induce significant...... apoptosis in the absence of p53. Furthermore, this apoptotic function of E2F-1 is separable from the ability to accelerate entry into DNA synthesis. Analysis of E2F-1 mutants indicates that although DNA-binding is required, transcriptional transactivation is not necessary for the induction of apoptosis by E...

  9. E2F1 in renal cancer: Mr Hyde disguised as Dr Jekyll?

    Science.gov (United States)

    Tian, Weihua; Cui, Fenggong; Esteban, Miguel A

    2013-10-01

    The transcription factor E2F1 has both oncogenic and tumour suppressor properties, depending on the context. Clarifying the function of E2F1 in different types of cancer is relevant because in those situations in which it acts as an oncogene there may be a route for therapeutic interference. Renal cell carcinoma is the most frequent form of kidney cancer in adults and inactivation of the von Hippel-Lindau (VHL) gene underlies most cases. This malignancy represents a challenge for standard therapies due to drug- and radio-resistance, effects that fit well within the scope of functions of E2F1. A new report by Mans et al postulates that up-regulation of E2F1 in VHL-defective renal cell carcinoma induces cell senescence and can thus be considered a good prognostic factor. Here we discuss these findings in a wider context and propose that E2F1 may actually not play a uniform role in renal cell carcinoma but rather an ambiguous one whose deeper understanding could have practical implications. Copyright © 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

  10. Synergistic cooperation of MDM2 and E2F1 contributes to TAp73 transcriptional activity

    Energy Technology Data Exchange (ETDEWEB)

    Kasim, Vivi, E-mail: vivikasim78@gmail.com [The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Huang, Can; Zhang, Jing; Jia, Huizhen; Wang, Yunxia [The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Yang, Li [The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); The 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044 (China); Miyagishi, Makoto [Molecular Composite Medicine Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566 (Japan); Wu, Shourong, E-mail: shourongwu@hotmail.com [The Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044 (China); The 111 Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing University, Chongqing 400044 (China)

    2014-07-04

    Highlights: • MDM2 is a novel positive regulator of TAp73 transcriptional activity. • MDM2 colocalizes together and physically interacts with E2F1. • Synergistic cooperation of MDM2 and E2F1 is crucial for TAp73 transcription. • MDM2 regulates TAp73 transcriptional activity in a p53-independent manner. - Abstract: TAp73, a structural homologue of p53, plays an important role in tumorigenesis. E2F1 had been reported as a transcriptional regulator of TAp73, however, the detailed mechanism remains to be elucidated. Here we reported that MDM2-silencing reduced the activities of the TAp73 promoters and the endogenous TAp73 expression level significantly; while MDM2 overexpression upregulated them. We further revealed that the regulation of TAp73 transcriptional activity occurs as a synergistic effect of MDM2 and E2F1, most probably through their physical interaction in the nuclei. Furthermore, we also suggested that MDM2 might be involved in DNA damage-induced TAp73 transcriptional activity. Finally, we elucidated that MDM2-silencing reduced the proliferation rate of colon carcinoma cells regardless of the p53 status. Our data show a synergistic effect of MDM2 and E2F1 on TAp73 transcriptional activity, suggesting a novel regulation pathway of TAp73.

  11. The CYP51F1 Gene of Leptographium qinlingensis: Sequence Characteristic, Phylogeny and Transcript Levels

    Directory of Open Access Journals (Sweden)

    Lulu Dai

    2015-05-01

    Full Text Available Leptographium qinlingensis is a fungal associate of the Chinese white pine beetle (Dendroctonus armandi and a pathogen of the Chinese white pine (Pinus armandi that must overcome the terpenoid oleoresin defenses of host trees. L. qinlingensis responds to monoterpene flow with abundant mechanisms that include export and the use of these compounds as a carbon source. As one of the fungal cytochrome P450 proteins (CYPs, which play important roles in general metabolism, CYP51 (lanosterol 14-α demethylase can catalyze the biosynthesis of ergosterol and is a target for antifungal drug. We have identified an L. qinlingensis CYP51F1 gene, and the phylogenetic analysis shows the highest homology with the 14-α-demethylase sequence from Grosmannia clavigera (a fungal associate of Dendroctonus ponderosae. The transcription level of CYP51F1 following treatment with terpenes and pine phloem extracts was upregulated, while using monoterpenes as the only carbon source led to the downregulation of CYP5F1 expression. The homology modeling structure of CYP51F1 is similar to the structure of the lanosterol 14-α demethylase protein of Saccharomyces cerevisiae YJM789, which has an N-terminal membrane helix 1 (MH1 and transmembrane helix 1 (TMH1. The minimal inhibitory concentrations (MIC of terpenoid and azole fungicides (itraconazole (ITC and the docking of terpenoid molecules, lanosterol and ITC in the protein structure suggested that CYP51F1 may be inhibited by terpenoid molecules by competitive binding with azole fungicides.

  12. 26 CFR 1.167(f)-1 - Reduction of salvage value taken into account for certain personal property.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Reduction of salvage value taken into account for certain personal property. 1.167(f)-1 Section 1.167(f)-1 Internal Revenue INTERNAL REVENUE SERVICE... for Individuals and Corporations § 1.167(f)-1 Reduction of salvage value taken into account for...

  13. 40 CFR Table F-1 to Subpart F of... - Performance Specifications for PM2.5 Class II Equivalent Samplers

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 5 2010-07-01 2010-07-01 false Performance Specifications for PM2.5 Class II Equivalent Samplers F Table F-1 to Subpart F of Part 53 Protection of Environment ENVIRONMENTAL..., Subpt. F, Table F-1 Table F-1 to Subpart F of Part 53—Performance Specifications for PM2.5 Class...

  14. Conditional E2F1 activation in transgenic mice causes testicular atrophy and dysplasia mimicking human CIS

    DEFF Research Database (Denmark)

    Agger, Karl; Santoni-Rugiu, Eric; Holmberg, Christian;

    2005-01-01

    E2F1 is a crucial downstream effector of the retinoblastoma protein (pRB) pathway. To address the consequences of short-term increase in E2F1 activity in adult tissues, we generated transgenic mice expressing the human E2F1 protein fused to the oestrogen receptor (ER) ligand-binding domain...

  15. File list: Oth.ALL.50.Pou2f1.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.ALL.50.Pou2f1.AllCell mm9 TFs and others Pou2f1 All cell types SRX877595,SRX877...592 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.ALL.50.Pou2f1.AllCell.bed ...

  16. BMW Sauber F1 Team 车队专用——BMW X PUMA

    Institute of Scientific and Technical Information of China (English)

    Brian

    2007-01-01

    Puma x F1.第一时间会想起Puma与法拉利车队合作的法拉利系列.还有,Puma更与法拉利签下长期合作协议。可是.最近却杀出个程咬金-BMW Sauber F1 Team车队,说的是Puma与宝马车队联合设计的新系.当中最注目的有这双Kart Cat Ⅱ F1赛车鞋.鞋形延用Puma Cat一贯的流线形设计,除了用上宝马车队专用的宝蓝色外.中底更用上Puma CeⅡ蜂巢缓震系统,算是向法拉利车队示威吧!

  17. Optical pumping effect in absorption imaging of F =1 atomic gases

    Science.gov (United States)

    Kim, Sooshin; Seo, Sang Won; Noh, Heung-Ryoul; Shin, Y.

    2016-08-01

    We report our study of the optical pumping effect in absorption imaging of 23Na atoms in the F =1 hyperfine spin states. Solving a set of rate equations for the spin populations in the presence of a probe beam, we obtain an analytic expression for the optical signal of the F =1 absorption imaging. Furthermore, we verify the result by measuring the absorption spectra of 23Na Bose-Einstein condensates prepared in various spin states with different probe-beam pulse durations. The analytic result can be used in the quantitative analysis of F =1 spinor condensate imaging and readily applied to other alkali-metal atoms with I =3 /2 nuclear spin such as 87Rb.

  18. 墨西哥考虑2010年重返F1

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    F1最早一次造访墨西哥是1962年,中间反复了两次,最后一次承办是1992年。墨西哥此后一直嚷嚷要重新承办F1,但这次是非常认真的,计划在2010年重返F1赛历。据来自墨西哥的消息称,有很多地方考虑新建赛道,其中呼声最高的是位于尤卡坦半岛的坎昆,另外两个地点是Puebla和Tijuana。

  19. Cat (Fel d 1) and dog (Can f 1) allergen levels in cars, dwellings and schools.

    Science.gov (United States)

    Niesler, A; Ścigała, G; Łudzeń-Izbińska, B

    Pets are an important source of indoor allergens. The aim of the study was to compare cat and dog allergen levels in cars, schools and homes. The study was carried out in 17 cars, 14 classrooms and 19 dwellings located in the highly industrialized and urbanized region of Poland. Dust and air samples were analyzed for Fel d 1 and Can f 1 using a double monoclonal ELISA assay. The highest amounts of cat and dog allergens (Fel d 1: 1169 μg/g; Can f 1: 277 μg/g) were found in dwellings with pets. Allergen concentrations were correlated with the number of animals kept at home. Although concentrations on automobile seats were lower, Fel d 1 levels exceeded 8 μg/g in 23.5 % of cars and high levels of Can f 1 (>10 μg/g) were found in 17.6 % of cars. The study revealed that cars of pet owners may be reservoirs of cat and dog allergens even when animals are not transported in them. In schools, concentrations of pet allergens did not reach high levels, but the moderate levels of Fel d 1 (≥1-8 μg/g) and Can f 1 (≥2-10 μg/g) were detected in 42.9 and 7.1 % of the investigated classrooms. Concentrations of cat and dog allergen in schools were higher than in homes without pets. While airborne Fel d 1 and Can f 1 levels were found low, residential allergen concentrations in settled dust and air were correlated. The study results suggest that classrooms and cars of pet owners may be important sites of exposure to cat and dog allergens, though the highest concentrations of Fel d 1 and Can f 1 are found in homes of pet owners.

  20. Operating principles of rotary molecular motors: differences between F1 and V1 motors.

    Science.gov (United States)

    Yamato, Ichiro; Kakinuma, Yoshimi; Murata, Takeshi

    2016-01-01

    Among the many types of bioenergy-transducing machineries, F- and V-ATPases are unique bio- and nano-molecular rotary motors. The rotational catalysis of F1-ATPase has been investigated in detail, and molecular mechanisms have been proposed based on the crystal structures of the complex and on extensive single-molecule rotational observations. Recently, we obtained crystal structures of bacterial V1-ATPase (A3B3 and A3B3DF complexes) in the presence and absence of nucleotides. Based on these new structures, we present a novel model for the rotational catalysis mechanism of V1-ATPase, which is different from that of F1-ATPases.

  1. An approximation for zero-balanced Appell function $F_1$ near $(1,1)$

    OpenAIRE

    Karp, D.

    2007-01-01

    We suggest an approximation for the zero-balanced Appell hypergeometric function $F_1$ near the singular point $(1,1)$. Our approximation can be viewed as a generalization of Ramanujan's approximation for zero-balanced ${_2F_1}$ and is expressed in terms of ${_3F_2}$. We find an error bound and prove some basic properties of the suggested approximation which reproduce the similar properties of the Appell function. Our approximation reduces to the approximation of Carlson-Gustafson when the Ap...

  2. HMGA2 induces pituitary tumorigenesis by enhancing E2F1 activity

    DEFF Research Database (Denmark)

    Fedele, Monica; Visone, Rosa; De Martino, Ivana

    2006-01-01

    HMGA2 gene amplification and overexpression in human prolactinomas and the development of pituitary adenomas in HMGA2 transgenic mice showed that HMGA2 plays a crucial role in pituitary tumorigenesis. We have explored the pRB/E2F1 pathway to investigate the mechanism by which HMGA2 acts. Here we......2 mice. Thus, HMGA2-mediated E2F1 activation is a crucial event in the onset of these tumors in transgenic mice and probably also in human prolactinomas....

  3. Engineering a light-controlled F1 ATPase using structure-based protein design

    OpenAIRE

    2016-01-01

    The F1 sub-complex of ATP synthase is a biological nanomotor that converts the free energy of ATP hydrolysis into mechanical work with an astonishing efficiency of up to 100% (Kinosita et al., 2000). To probe the principal mechanics of the machine, I re-engineered the active site of E.coli F1 ATPase with a structure-based protein design approach: by incorporation of a site-specific, photoswitchable crosslinker, whose end-to-end distance can be modulated by illumination with light of two diffe...

  4. 山核桃与薄壳山核桃种间杂交F1代果实及苗木性状变异分析%Genetic analysis on fruits and seedlings characters variation of F1 between Carya cathayensis and C. illinoensis

    Institute of Scientific and Technical Information of China (English)

    王年金; 陈军; 姜俊马; 刘荣昌; 姚小华; 王开良; 王国财

    2011-01-01

    The main characters of fruits and seedlings of F1 obtained by hybridization between male parent Corya illinoensis and female parent Carya cathayensis were examined. The results showed that characters of F1 were significantly different from that of control in terms of weight and size of fruit and seed, height and diameter of seedlings growth. The weight, height and diameter of fruits were higher than the control by 29. 1%, 7.2 %, 11.0 %, respectively. And the weight, height and diameter of seed were higher than the control by 30. 9 % ,8. 13 % ,12.4 %, respectively. The average height and diameter of 3 years old seedlings were higher than the control by 18. 1% and 19. 7 %. And it could achieve 29. 2 % and 32. 81% after having planted for2 years. Therefore, It was effective to increase fruit size and improve characters through interspecific hybridization. Seedlings with double embryos in F1 generation accounted for 10 % which was 5 times higher than the control.%以薄壳山核桃为父本,山核桃为母本,进行种间杂交,对F1代果实及苗木主要性状进行测定,结果表明:杂交F1代果实与果核(种子)的大小、质量以及苗木的高、径生长与对照之间都存在显著水平或极显著水平的差异.其中单果质量、果高、果径分别比对照高29.1%、7.2%、11.0%;单核质量、核高、核径分别比对照高30.9%、8.13%、12.4%;3年生苗木平均高和地径比对照分别高18.1%和19.7%;造林2 a后苗木平均高和平均地径比对照分别高29.2%和32.81%.另外,杂交F1代双胚苗所占比率为10%,是对照的5倍.研究表明,种间杂交对增大山核桃果体、改良山核桃性状是有意义的.

  5. E2F1-Mediated Induction of NFYB Attenuates Apoptosis via Joint Regulation of a Pro-Survival Transcriptional Program.

    Directory of Open Access Journals (Sweden)

    Xiaolei Jiang

    Full Text Available The E2F1 transcription factor regulates cell proliferation and apoptosis through the control of a considerable variety of target genes. Previous work has detailed the role of other transcription factors in mediating the specificity of E2F function. Here we identify the NF-YB transcription factor as a novel direct E2F1 target. Genome-wide expression analysis of the effects of NFYB knockdown on E2F1-mediated transcription identified a large group of genes that are co-regulated by E2F1 and NFYB. We also provide evidence that knockdown of NFYB enhances E2F1-induced apoptosis, suggesting a pro-survival function of the NFYB/E2F1 joint transcriptional program. Bioinformatic analysis suggests that deregulation of these NFY-dependent E2F1 target genes might play a role in sarcomagenesis as well as drug resistance.

  6. Measurement of growth curve in F1 generation of Rongshui miniature pig%融水小型猪 F1代生长研究

    Institute of Scientific and Technical Information of China (English)

    施赫赫; 陈淦; 刘运忠; 刘科; 邝少松; 任海涛; 余细勇; 唐小江

    2015-01-01

    目的:测定融水小型猪F1代体重和体尺。方法选取F1代融水小型猪83头(雌性48头,雄性35头),测定初生至12月龄的体重、体长、体高、胸围、胸宽、胸深、管围、腿围、嘴裂长度共9个生长发育指标,并应用SPSS统计软件和Logistic非线性生长模型进行分析。结果融水小型猪F1代的初生体重雌雄分别为0.61±0.14 kg和0.55±0.13 kg,6月龄体重雌雄分别为17.21±5.20 kg和16.35±5.23 kg,12月龄体重雌雄分别为26.97±6.49 kg和26.53±5.65 kg。雌雄比较,9项指标所测结果接近,除了初生体重和体长、10月龄胸宽有差异( P <0.05),其余指标同月龄雌雄之间均无明显差异。应用Logistic模型分析,体重生长拐点在5~6月龄间,体长和腿围生长拐点在2~3月龄间,体高、胸围、胸宽、胸深、管围和嘴裂长度的生长拐点在1~2月龄间。结论融水小型猪F1代成年体重轻,性情温顺,具备培养成实验用小型猪基本条件。%Objective To measure the body weight and body size of the F1 generation in Rongshui miniature pig ( RMP ).Methods 83 F1 generations of RMPs (48 females and 35 males) were selected randomly.9 traits included body-weight, body-length, body-height, chest-circumference, chest-breadth, chest-depth, circum of pastern, girth of leg and rictus were measured, and analyzed statistically by SPSS statistical software and Logistic nonlinear growth analysis model.Results In the F1 generations of RMP, the weights of birth day、6thmonth and 12th month of female and male were 0.61 ±0.14 kg and 0.55 ±0.13 kg, 17.21 ±5.20 kg and 16.35 ±5.23 kg, 26.97 ±6.49 kg and 26.53 ±5.65 kg respectively.There was no difference significantly between the genders of the 9 measured traits except for born-weight, born-length and chest-breadth in 10th month ( P <0.05 ).According to the analysis in Logistic model, body-weight inflection point was between 5th -6th month, body length

  7. Structures of the thermophilic F1-ATPase epsilon subunit suggesting ATP-regulated arm motion of its C-terminal domain in F1.

    Science.gov (United States)

    Yagi, Hiromasa; Kajiwara, Nobumoto; Tanaka, Hideaki; Tsukihara, Tomitake; Kato-Yamada, Yasuyuki; Yoshida, Masasuke; Akutsu, Hideo

    2007-07-03

    The epsilon subunit of bacterial and chloroplast F(o)F(1)-ATP synthases modulates their ATP hydrolysis activity. Here, we report the crystal structure of the ATP-bound epsilon subunit from a thermophilic Bacillus PS3 at 1.9-A resolution. The C-terminal two alpha-helices were folded into a hairpin, sitting on the beta sandwich structure, as reported for Escherichia coli. A previously undescribed ATP binding motif, I(L)DXXRA, recognizes ATP together with three arginine and one glutamate residues. The E. coli epsilon subunit binds ATP in a similar manner, as judged on NMR. We also determined solution structures of the C-terminal domain of the PS3 epsilon subunit and relaxation parameters of the whole molecule by NMR. The two helices fold into a hairpin in the presence of ATP but extend in the absence of ATP. The latter structure has more helical regions and is much more flexible than the former. These results suggest that the epsilon C-terminal domain can undergo an arm-like motion in response to an ATP concentration change and thereby contribute to regulation of F(o)F(1)-ATP synthase.

  8. 粤优丝瓜的选育%A New Sponge Gourd F1 Hybrid-‘Yueyousigua’

    Institute of Scientific and Technical Information of China (English)

    罗剑宁; 何晓莉; 罗少波; 郑晓明; 张长远; 龚浩

    2005-01-01

    粤优丝瓜是以汕头丝瓜自交系D2为母本,以雅岗丝瓜自交系S12为父本配制而成的一代杂种.早熟,植株生长势及分枝力强,坐果率高,果实棍棒形,匀称,皮色绿白,瓜纵径49.0 cm、横径4.8 cm,肉厚0.77 cm,单瓜质量0.33 kg,味清甜,每667 m2产量2000~2 500kg.抗枯萎病,高抗疫病,耐霜霉病,适宜广东省春秋种植.

  9. The transcription factor E4F1 coordinates CHK1-dependent checkpoint and mitochondrial functions.

    Science.gov (United States)

    Rodier, Geneviève; Kirsh, Olivier; Baraibar, Martín; Houlès, Thibault; Lacroix, Matthieu; Delpech, Hélène; Hatchi, Elodie; Arnould, Stéphanie; Severac, Dany; Dubois, Emeric; Caramel, Julie; Julien, Eric; Friguet, Bertrand; Le Cam, Laurent; Sardet, Claude

    2015-04-14

    Recent data support the notion that a group of key transcriptional regulators involved in tumorigenesis, including MYC, p53, E2F1, and BMI1, share an intriguing capacity to simultaneously regulate metabolism and cell cycle. Here, we show that another factor, the multifunctional protein E4F1, directly controls genes involved in mitochondria functions and cell-cycle checkpoints, including Chek1, a major component of the DNA damage response. Coordination of these cellular functions by E4F1 appears essential for the survival of p53-deficient transformed cells. Acute inactivation of E4F1 in these cells results in CHK1-dependent checkpoint deficiency and multiple mitochondrial dysfunctions that lead to increased ROS production, energy stress, and inhibition of de novo pyrimidine synthesis. This deadly cocktail leads to the accumulation of uncompensated oxidative damage to proteins and extensive DNA damage, ending in cell death. This supports the rationale of therapeutic strategies simultaneously targeting mitochondria and CHK1 for selective killing of p53-deficient cancer cells.

  10. Cleanup Verification Package for the 126-F-1, 184-F Powerhouse Ash Pit

    Energy Technology Data Exchange (ETDEWEB)

    S. W. Clark and H. M. Sulloway

    2007-09-26

    This cleanup verification package documents completion of remedial action for the 126-F-1, 184-F Powerhouse Ash Pit. This waste site received coal ash from the 100-F Area coal-fired steam plant. Leakage of process effluent from the 116-F-14 , 107-F Retention Basins flowed south into the ash pit, contaminating the northern portion.

  11. 76 FR 28997 - Extension of Employment Authorization for Haitian F-1 Nonimmigrant Students Experiencing Severe...

    Science.gov (United States)

    2011-05-19

    ... earthquake. See 75 FR 3476. Haiti has limited resources to cope with a natural disaster like this earthquake... students whose country of citizenship is Haiti and who are experiencing severe economic hardship as a... requirements governing on-campus and off-campus employment for F-1 nonimmigrant students whose country...

  12. F1 rotary motor of ATP synthase is driven by the torsionally-asymmetric drive shaft

    Science.gov (United States)

    Kulish, O.; Wright, A. D.; Terentjev, E. M.

    2016-06-01

    F1F0 ATP synthase (ATPase) either facilitates the synthesis of ATP in a process driven by the proton moving force (pmf), or uses the energy from ATP hydrolysis to pump protons against the concentration gradient across the membrane. ATPase is composed of two rotary motors, F0 and F1, which compete for control of their shared γ -shaft. We present a self-consistent physical model of F1 motor as a simplified two-state Brownian ratchet using the asymmetry of torsional elastic energy of the coiled-coil γ -shaft. This stochastic model unifies the physical concepts of linear and rotary motors, and explains the stepped unidirectional rotary motion. Substituting the model parameters, all independently known from recent experiments, our model quantitatively reproduces the ATPase operation, e.g. the ‘no-load’ angular velocity is ca. 400 rad/s anticlockwise at 4 mM ATP. Increasing the pmf torque exerted by F0 can slow, stop and overcome the torque generated by F1, switching from ATP hydrolysis to synthesis at a very low value of ‘stall torque’. We discuss the motor efficiency, which is very low if calculated from the useful mechanical work it produces - but is quite high when the ‘useful outcome’ is measured in the number of H+ pushed against the chemical gradient.

  13. E. coli F1-ATPase interacts with a membrane protein component of a proton channel.

    Science.gov (United States)

    Walker, J E; Saraste, M; Gay, N J

    1982-08-26

    The ATP synthases of bacteria, mitochondria and chloroplasts, which use the energy of a transmembrane proton gradient to power the synthesis of ATP, consist of an integral membrane component F0--thought to contain a proton channel--and a catalytic component, F1. To help investigate the way F0 and F1 are coupled, we have sequenced the b-subunit of the Escherichia coli F0, which seems to be the counterpart of a thermophilic bacteria F0 subunit thought to be essential for F1 binding. We report here that its sequence is remarkable, being hydrophobic around the N-terminus and highly charged in the remainder. We propose that the N-terminal segment lies in the membrane and the rest outside. The extramembranous section contains two adjacent stretches of 31 amino acids where the sequence is very similar: in the second of these stretches there is further internal homology. These duplicated stretches of the polypeptide probably fold into two alpha-helices which have many common features able to make contact with F1 subunits. Thus protein b occupies a central position in the enzyme, where it may be involved in proton translocation. It is possibly also important in biosynthetic assembly.

  14. Nucleotide occupancy of F1-ATPase catalytic sites under crystallization conditions.

    Science.gov (United States)

    Löbau, S; Weber, J; Senior, A E

    1997-03-03

    Using site-directed tryptophan fluorescence we studied nucleotide occupancy of the catalytic sites of Escherichia coli F1-ATPase, under conditions used previously for crystallization and X-ray structure analysis of the bovine mitochondrial enzyme [Abrahams et al. (1994) Nature 370, 621-628]. We found that only two of the three catalytic sites were filled in the E. coli enzyme under these conditions (250 microM MgAMPPNP plus 5 microM MgADP), consistent with what was reported in the bovine F1 X-ray structure. However, subsequent addition of a physiological concentration of MgATP readily filled the third catalytic site. Therefore the enzyme form seen in the X-ray structure results from the fact that it is obtained under sub-saturating nucleotide conditions. The data show that the X-ray structure is compatible with a catalytic mechanism in which all three F1-ATPase catalytic sites must fill with MgATP to initiate steady-state hydrolysis [e.g. Weber and Senior (1996) Biochim. Biophys. Acta 1275, 101-104]. The data further demonstrate that the site-directed tryptophan fluorescence technique can provide valuable support for F1 crystallography studies.

  15. M2-F1 in flight over lakebed on tow line

    Science.gov (United States)

    1963-01-01

    Following the first M2-F1 airtow flight on 16 August 1963, the Flight Research Center used the vehicle for both research flights and to check out new lifting-body pilots. These included Bruce Peterson, Don Mallick, Fred Haise, and Bill Dana from NASA. Air Force pilots who flew the M2-F1 included Chuck Yeager, Jerry Gentry, Joe Engle, Jim Wood, and Don Sorlie, although Wood, Haise, and Engle only flew on car tows. In the three years between the first and last flights of the M2-F1, it made about 400 car tows and 77 air tows. The wingless, lifting body aircraft design was initially concieved as a means of landing an aircraft horizontally after atmospheric reentry. The absence of wings would make the extreme heat of re-entry less damaging to the vehicle. In 1962, Dryden management approved a program to build a lightweight, unpowered lifting body as a prototype to flight test the wingless concept. It would look like a 'flying bathtub,' and was designated the M2-F1, the 'M' referring to 'manned' and 'F' referring to 'flight' version. It featured a plywood shell placed over a tubular steel frame crafted at Dryden. Construction was completed in 1963. The first flight tests of the M2-F1 were over Rogers Dry Lake at the end of a tow rope attached to a hopped-up Pontiac convertible driven at speeds up to about 120 mph. This vehicle needed to be able to tow the M2-F1 on the Rogers Dry Lakebed adjacent to NASA's Flight Research Center (FRC) at a minimum speed of 100 miles per hour. To do that, it had to handle the 400-pound pull of the M2-F1. Walter 'Whitey' Whiteside, who was a retired Air Force maintenance officer working in the FRC's Flight Operations Division, was a dirt-bike rider and hot-rodder. Together with Boyden 'Bud' Bearce in the Procurement and Supply Branch of the FRC, Whitey acquired a Pontiac Catalina convertible with the largest engine available. He took the car to Bill Straup's renowned hot-rod shop near Long Beach for modification. With a special gearbox and

  16. KERAGAAN PERTUMBUHAN DAN VARIASI GENETIK ABALON Haliotis squamata Reeve (1846 HASIL SELEKSI F-1

    Directory of Open Access Journals (Sweden)

    Gusti Ngurah Permana

    2015-12-01

    Full Text Available Produksi benih abalon Haliotis squamata skala massal di hatcheri telah berhasil dilakukan di Balai Besar Penelitian dan Pengembangan Budidaya Laut Gondol, Bali. Permasalahan utama dalam budidaya abalon adalah pertumbuhan yang lambat. Keadaan tersebut diduga karena pengaruh faktor genetik dan lingkungan. Penelitian ini bertujuan mengetahui keragaan pertumbuhan dan variasi genetik abalon tumbuh cepat hasil seleksi individu. Hasil penelitian ini diketahui bahwa pembentukan populasi F-1 mempunyai pertumbuhan yang lebih baik dengan F-1 kontrol. Peningkatan bobot yang dicapai 22,15 g atau 17,93% lebih baik dibandingkan F-1 kontrol. Keragaman genetik F-1 terseleksi yang ditunjukkan dari nilai heterozigositas adalah (Ho. 0,023 terjadi penurunan 21,7% jika dibandingkan F-0. Hal ini dapat terjadi karena hilangnya beberapa allele dalam proses seleksi. Terdapat hubungan antara jumlah heterozigot pada lokus tertentu dengan pertumbuhan abalon. Hasil ini diharapkan dapat mendukung upaya meningkatkan produksi benih yang mempunyai performa fenotipe dan genotipe unggul sehingga dapat mendukung kegiatan budidaya abalon yang berkelanjutan.

  17. Data of evolutionary structure change: 1BU1F-1OOTA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1BU1F-1OOTA 1BU1 1OOT F A -IIVVALYDYEAIHHEDLSFQKGDQMVVLEES---GEWW...4> 1OOT A 1OOTA ...in> -2.4820001125335693 10.661999702453613 46.814998626708984 ...8 -0.6930000185966492 -0.30300000309944153 tion> 2.081804037094116 A 1OOTA WTGRV--NGREG

  18. E2F1 is crucial for E2F-dependent apoptosis

    DEFF Research Database (Denmark)

    Lazzerini Denchi, Eros; Helin, Kristian

    2005-01-01

    Loss of the retinoblastoma protein, pRB, leads to apoptosis, and several results have suggested that this is dependent on the E2F transcription factors. However, so far, the ability of the different E2F family members to contribute to apoptosis is controversial. Here, we show that ectopic...... expression of E2F3 results in apoptosis in both primary mouse fibroblasts and transgenic mice. Apoptosis induced by E2F3 is associated with the accumulation of E2F1 and, strikingly, we found that E2F3-induced apoptosis is dependent on E2F1. On the basis of these results, we propose that the accumulation...... of crucial levels of E2F1 activity, and not total E2F activity, is essential for the induction of apoptosis in response to a deregulated pRB pathway. These results are consistent with previous findings that E2F1, but not other E2Fs, can have tumour-suppressing activities....

  19. Less is more: reduced catechol production permits Pseudomonas putida F1 to grow on styrene.

    Science.gov (United States)

    George, Kevin W; Hay, Anthony

    2012-11-01

    Pseudomonas putida F1 is unable to grow on styrene due to the accumulation of 3-vinylcatechol, a toxic metabolite that is produced through the toluene degradation (tod) pathway and causes catechol-2,3-dioxygenase (C23O) inactivation. In this study, we characterized a spontaneous F1 mutant, designated SF1, which acquired the ability to grow on styrene and did not accumulate 3-vinylcatechol. Whereas adaptation to new aromatic substrates has typically been shown to involve increased C23O activity or the acquisition of resistance to C23O inactivation, SF1 retained wild-type C23O activity. Surprisingly, SF1 grew more slowly on toluene, its native substrate, and exhibited reduced toluene dioxygenase (TDO) activity (approximately 50 % of that of F1), the enzyme responsible for ring hydroxylation and subsequent production of 3-vinylcatechol. DNA sequence analysis of the tod operon of SF1 revealed a single base pair mutation in todA (C479T), a gene encoding the reductase component of TDO. Replacement of the wild-type todA allele in F1 with todA(C479T) reduced TDO activity to SF1 levels, obviated vinylcatechol accumulation, and conferred the ability to grow on styrene. This novel 'less is more' strategy - reduced catechol production as a means to expand growth substrate range - sheds light on an alternative approach for managing catechol toxicity during the metabolism of aromatic compounds.

  20. E2F1-mediated transcriptional inhibition of the plasminogen activator inhibitor type 1 gene

    DEFF Research Database (Denmark)

    Koziczak, M; Müller, H; Helin, K;

    2001-01-01

    -sensitive retinoblastoma protein (pRB), a shift to a permissive temperature induced PAI-1 mRNA expression. In U2OS cells stably expressing an E2F1-estrogen receptor chimeric protein that could be activated by tamoxifen, PAI-1 gene transcription was markedly reduced by tamoxifen even in the presence of cycloheximide...

  1. Inheritance of S-genotypes in Paviot × Kabaasi apricot F1 progenies

    Directory of Open Access Journals (Sweden)

    Zehra Tuğba Murathan

    2016-09-01

    Full Text Available Self-incompatibility plays an important role in the fertilization of fruit species such as apricot. Apricot (Prunus armeniaca L. shows gametophytic self-incompatibility, which is controlled by a multi-allelic S-locus. In this study, S-alleles of 77 F1 progenies derived from Paviot, which is one of the French local cultivars, and Kabaasi, one of the most important Turkish dried apricot cultivars, parents were identified by S-RNase intron regions polymerase chain reaction (PCR amplification and DNA sequencing. The results from the S-allele PCR analysis revealed that the Paviot female parent had an ScS2 genotype and the Kabaasi male parent had S1S9 alleles. Forty-three of the F1 progenies showed self-compatibility allele (Sc by having either ScS9 or ScS1 alleles. Thirty-four of the F1 progenies were self-incompatible by having either S2S1 or S2S9 alleles. The distributions of detected alleles in F1 progenies were determined as follows: ScS1 31.2%, S1S2 27.3%, ScS9 24.7% and S2S9 16.8%. The results from the study are relevant for the data obtained in apricot breeding programmes in the selection of crossing combinations and in the establishment of commercial orchards.

  2. The Transcription Factor E4F1 Coordinates CHK1-Dependent Checkpoint and Mitochondrial Functions

    Directory of Open Access Journals (Sweden)

    Geneviève Rodier

    2015-04-01

    Full Text Available Recent data support the notion that a group of key transcriptional regulators involved in tumorigenesis, including MYC, p53, E2F1, and BMI1, share an intriguing capacity to simultaneously regulate metabolism and cell cycle. Here, we show that another factor, the multifunctional protein E4F1, directly controls genes involved in mitochondria functions and cell-cycle checkpoints, including Chek1, a major component of the DNA damage response. Coordination of these cellular functions by E4F1 appears essential for the survival of p53-deficient transformed cells. Acute inactivation of E4F1 in these cells results in CHK1-dependent checkpoint deficiency and multiple mitochondrial dysfunctions that lead to increased ROS production, energy stress, and inhibition of de novo pyrimidine synthesis. This deadly cocktail leads to the accumulation of uncompensated oxidative damage to proteins and extensive DNA damage, ending in cell death. This supports the rationale of therapeutic strategies simultaneously targeting mitochondria and CHK1 for selective killing of p53-deficient cancer cells.

  3. 78 FR 69538 - Attestation Process for Employers Using F-1 Students in Off-Campus Work

    Science.gov (United States)

    2013-11-20

    ... workers, Employment, Employment and training, Enforcement, Forest and forest products, Fraud, Health professions, Immigration, Labor, Longshore and harbor work, Migrant workers, Nonimmigrant workers, Passports... employers seeking to hire F-1 foreign students as part-time workers off-campus. These subparts...

  4. In Utero Nutritional Manipulation Provokes Dysregulated Adipocytokines Production in F1 Offspring in Rats

    Directory of Open Access Journals (Sweden)

    Mervat Y. Hanafi

    2016-01-01

    Full Text Available Background. Intrauterine environment plays a pivotal role in the origin of fatal diseases such as diabetes. Diabetes and obesity are associated with low-grade inflammatory state and dysregulated adipokines production. This study aims to investigate the effect of maternal obesity and malnutrition on adipokines production (adiponectin, leptin, and TNF-α in F1 offspring in rats. Materials and Methods. Wistar rats were allocated in groups: F1 offspring of control mothers under control diet (CF1-CD and under high-fat diet (CF1-HCD, F1 offspring of obese mothers under CD (OF1-CD and under HCD (OF1-HCD, and F1 offspring of malnourished mothers under CD (MF1-CD and under HCD (MF1-HCD. Every 5 weeks postnatally, blood samples were obtained for biochemical analysis. Results. At the end of the 30-week follow-up, OF1-HCD and MF1-HCD exhibited hyperinsulinemia, moderate dyslipidemia, insulin resistance, and impaired glucose homeostasis compared to CF1-CD and CF1-HCD. OF1-HCD and MF1-HCD demonstrated low serum levels of adiponectin and high levels of leptin compared to CF1-CD and CF1-HCD. OF1-CD, OF1-HCD, and MF1-HCD had elevated serum levels of TNF-α compared to CF1-CD and CF1-HCD (p<0.05. Conclusion. Maternal nutritional manipulation predisposes the offspring to development of insulin resistance in their adult life, probably via instigating dysregulated adipokines production.

  5. Properties of F1-ATPase from the uncD412 mutant of Escherichia coli.

    Science.gov (United States)

    Wise, J G; Duncan, T M; Latchney, L R; Cox, D N; Senior, A E

    1983-11-01

    Properties of purified F1-ATPase from Escherichia coli mutant strain AN484 (uncD412) have been studied in an attempt to understand why the amino acid substitution in the beta-subunit of this enzyme causes a tenfold reduction from normal MgATP hydrolysis rate. In most properties that were studied, uncD412 F1-ATPase resembled normal E. coli F1-ATPase. Both enzymes were found to contain a total of six adenine-nucleotide-binding sites, of which three were found to be non-exchangeable and three were exchangeable (catalytic) sites. Binding of the non-hydrolysable substrate analogue adenosine 5'-[beta gamma-imido]triphosphate (p[NH]ppA) to the three exchangeable sites showed apparent negative co-operativity. The binding affinities for p[NH]ppA, and also ADP, at the exchangeable sites were similar in the two enzymes. Both enzymes were inhibited by efrapeptin, aurovertin and p[NH]ppA, and were inactivated by dicyclohexylcarbodi-imide, 4-chloro-7-nitrobenzofurazan and p-fluorosulphonyl-benzoyl-5'-adenosine. Km values for CaATP and MgATP were similar in the two enzymes. uncD412 F1-ATPase was abnormally unstable at high pH, and dissociated into subunits readily with consequent loss of activity. The reason for the impairment of catalysis in uncD412 F1-ATPase cannot be stated with certainty from these studies. However we discuss the possibility that the mutation interrupts subunit interaction, thereby causing a partial impairment in the site-site co-operativity which is required for 'promotion' of catalysis in this enzyme.

  6. Study on the polymorphism of POU1F1 gene in sheep

    Directory of Open Access Journals (Sweden)

    Jun Yan Bai

    Full Text Available ABSTRACT In this study, POU1F1 gene polymorphism was detected in five sheep populations (large-tailed Han, small-tailed Han, Yuxi fat-tailed, Lanzhou large-tailed, and Mongolian sheep, using DNA pooling and sequencing, to provide theoretical basis for the breeding of excellent sheep varieties. Three single-nucleotide polymorphism (SNP loci of POU1F1 gene were detected in five sheep populations, namely C355T (C/T, C71G (C/G, and C330G (C/G. C and T frequencies of C355T were 0.67/0.33, 0.81/0.19, 0.67/0.33, 1.00/0.00, and 0.93/0.07, respectively, in large-tailed Han, small-tailed Han, Yuxi fat-tailed, Mongolian, and Lanzhou large-tailed sheep. C of C355T locus was the dominant allele in five sheep populations. C and G allele frequencies of C330G locus were detected in Yuxi fat-tailed sheep; their frequencies were 0.75 and 0.25, respectively. C and G allele of C71G locus were only detected in Yuxi fat-tailed and large-tailed Han sheep; their frequencies were 0.87/0.13 and 0.87/0.13, respectively. The cluster analysis based on POU1F1 gene sequence showed that bactrian camel, dromedary, and wild camel clustered first, and dolphin and killer whales clustered according to taxonomy. Although the four species Tibetan antelope, buffalo, goat, and sheep were alone, they got close and the relative genetic relationship was intimate according to the dendrogram. The mutation site analysis of the POU1F1 gene in five sheep populations in this study would be favorable for uncovering the function of POU1F1 gene deeply.

  7. Resveratrol enhances the radiosensitivity of nasopharyngeal carcinoma cells by downregulating E2F1.

    Science.gov (United States)

    Tan, Yuhui; Wei, Xianli; Zhang, Wenyin; Wang, Xiaolan; Wang, Kun; Du, Biaoyan; Xiao, Jianyong

    2017-03-01

    Identification of safe, effective radiosensitizing agents is urgently needed to improve the outcome of radiotherapy in nasopharyngeal cancer (NPC). In this study, we assessed the ability of the polyphenol resveratrol to act as a radiosensitizer in vitro and in vivo. CNE-1 cells were treated with 50 µM resveratrol for 24 h, then irradiated. E2F transcription factor 1 (E2F1) was stably knocked down and overexpressed using lentiviruses. A xenograft model of NPC was established in nude mice using CNE-1 cells. Compared to control DMSO‑treated CNE-1 cells, resveratrol inhibited colony-forming ability and induced G1 phase cell cycle arrest. Radiation survival curves confirmed resveratrol significantly sensitized CNE-1 cells, and resveratrol in combination with 2 Gy irradiation synergistically increased apoptosis. Immunoblotting showed resveratrol dose- and time-dependently downregulated E2F1 and phospho-AKT (p-AKT). Knockdown of E2F1 significantly increased radiosensitivity and downregulated p-AKT; overexpression of E2F1 reversed resveratrol-induced radiosensitivity and upregulated p-AKT. In vivo, 50 mg/kg/day resveratrol and 4 Gy irradiation led to significantly lower tumor volume and tumor weight compared to resveratrol or irradiation alone. Our findings show that resveratrol increases the radiosensitivity of NPC cells by downregulating E2F1 and inhibiting p-AKT, and therefore has potential as a radiosensitizer for NPC.

  8. Source-Sink Relationship in Intersubspecific Hybrid Rice

    Institute of Scientific and Technical Information of China (English)

    LI Ji-hang; XIANG Xun-chao; HE Li-bin; LI Ping

    2006-01-01

    Three indica restorer lines (Mianhui 725, Shuhui 527, Shuhui 881), an American rice variety Lemont and a javanica rice variety Xiangdali were crossed with japonica Kitaake, and five F1 hybrids were obtained to study the photosynthetic and agronomic traits. The data on photosynthetic characteristics indicated that the net photosynthetic rate (Pn) of the five F1 hybrids was significantly higher than that of their parents (or one of them) under high photosynthetic flux density (PFD); while the overall performance of hybrids was better than their respective parents in apparent quantum yield (AQY), carboxylation efficiency (CE) and CO2compensation point (CCP). Moreover, the photosynthetic performance of the five F1 were different due to the variation in heredity and the typical indica-japonica hybrids, Mianhui 725/Kitaake and Shuhui 527/Kitaake, were better than the others on this aspect. The agronomic traits revealed that the five F1 exhibited different heterosis, with Shuhui 881/Kitaake the largest sink followed by Mianhui725/Kitaake, Shuhui 527/Kitaake, Lemont/Kitaake and Xiangdaii/Kitaake. The production potential of indica-japonica hybrids was higher than that of the other two hybrids, which was consistent with the performance of Pn. However, the superior trait of indica-japonica hybrids on sink size has not been fully turned into high yield because of abnormal seed setting. Therefore, attention should be paid to the proper genome coordination and appropriate genetic distance so as to achieve super high yielding.

  9. E2F1 promote the aggressiveness of human colorectal cancer by activating the ribonucleotide reductase small subunit M2

    Energy Technology Data Exchange (ETDEWEB)

    Fang, Zejun [Sanmen People' s Hospital of Zhejiang, Sanmen, Zhejiang, 317100 (China); Gong, Chaoju [Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058 (China); Liu, Hong [Zhejiang Normal University – Jinhua People' s Hospital Joint Center for Biomedical Research, Jinhua, Zhejiang, 321004 (China); Zhang, Xiaomin; Mei, Lingming [Sanmen People' s Hospital of Zhejiang, Sanmen, Zhejiang, 317100 (China); Song, Mintao [Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS), School of Basic Medicine, Peking Union Medical College (PUMC), Beijing, 100005 (China); Qiu, Lanlan; Luo, Shuchai; Zhu, Zhihua; Zhang, Ronghui; Gu, Hongqian [Sanmen People' s Hospital of Zhejiang, Sanmen, Zhejiang, 317100 (China); Chen, Xiang, E-mail: sychenxiang@126.com [Sanmen People' s Hospital of Zhejiang, Sanmen, Zhejiang, 317100 (China)

    2015-08-21

    As the ribonucleotide reductase small subunit, the high expression of ribonucleotide reductase small subunit M2 (RRM2) induces cancer and contributes to tumor growth and invasion. In several colorectal cancer (CRC) cell lines, we found that the expression levels of RRM2 were closely related to the transcription factor E2F1. Mechanistic studies were conducted to determine the molecular basis. Ectopic overexpression of E2F1 promoted RRM2 transactivation while knockdown of E2F1 reduced the levels of RRM2 mRNA and protein. To further investigate the roles of RRM2 which was activated by E2F1 in CRC, CCK-8 assay and EdU incorporation assay were performed. Overexpression of E2F1 promoted cell proliferation in CRC cells, which was blocked by RRM2 knockdown attenuation. In the migration and invasion tests, overexpression of E2F1 enhanced the migration and invasion of CRC cells which was abrogated by silencing RRM2. Besides, overexpression of RRM2 reversed the effects of E2F1 knockdown partially in CRC cells. Examination of clinical CRC specimens demonstrated that both RRM2 and E2F1 were elevated in most cancer tissues compared to the paired normal tissues. Further analysis showed that the protein expression levels of E2F1 and RRM2 were parallel with each other and positively correlated with lymph node metastasis (LNM), TNM stage and distant metastasis. Consistently, the patients with low E2F1 and RRM2 levels have a better prognosis than those with high levels. Therefore, we suggest that E2F1 can promote CRC proliferation, migration, invasion and metastasis by regulating RRM2 transactivation. Understanding the role of E2F1 in activating RRM2 transcription will help to explain the relationship between E2F1 and RRM2 in CRC and provide a novel predictive marker for diagnosis and prognosis of the disease. - Highlights: • E2F1 promotes RRM2 transactivation in CRC cells. • E2F1 promotes the proliferation of CRC cells by activating RRM2. • E2F1 promotes the migration and

  10. Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna.

    Directory of Open Access Journals (Sweden)

    Nur Syafiqah Mohamad Ishak

    Full Text Available Identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. Orthologs of Drosophila orphan receptor Fushi tarazu factor-1 (Ftz-F1 are known to function in genetic sex determination. In contrast, their roles in environmental sex determination remain unknown. In this study, we have cloned and characterized the Ftz-F1 ortholog in the branchiopod crustacean Daphnia magna, which produces males in response to environmental stimuli. Similar to that observed in Drosophila, D. magna Ftz-F1 (DapmaFtz-F1 produces two splicing variants, αFtz-F1 and βFtz-F1, which encode 699 and 777 amino acids, respectively. Both isoforms share a DNA-binding domain, a ligand-binding domain, and an AF-2 activation domain and differ only at the A/B domain. The phylogenetic position and genomic structure of DapmaFtz-F1 suggested that this gene has diverged from an ancestral gene common to branchiopod crustacean and insect Ftz-F1 genes. qRT-PCR showed that at the one cell and gastrulation stages, both DapmaFtz-F1 isoforms are two-fold more abundant in males than in females. In addition, in later stages, their sexual dimorphic expressions were maintained in spite of reduced expression. Time-lapse imaging of DapmaFtz-F1 RNAi embryos was performed in H2B-GFP expressing transgenic Daphnia, demonstrating that development of the RNAi embryos slowed down after the gastrulation stage and stopped at 30-48 h after ovulation. DapmaFtz-F1 shows high homology to insect Ftz-F1 orthologs based on its amino acid sequence and exon-intron organization. The sexually dimorphic expression of DapmaFtz-F1 suggests that it plays a role in environmental sex determination of D. magna.

  11. Purification, crystallization, and properties of F1-ATPase complexes from the thermoalkaliphilic Bacillus sp. strain TA2.A1.

    Science.gov (United States)

    Stocker, Achim; Keis, Stefanie; Cook, Gregory M; Dimroth, Peter

    2005-11-01

    Recently, we reported the cloning of the atp operon encoding for the F(1)F(0)-ATP synthase from the extremely thermoalkaliphilic bacterium Bacillus sp. strain TA2.A1. In this study, the genes encoding the F(1) moiety of the enzyme complex were cloned from the atp operon into the vector pTrc99A and expressed in Escherichia coli in two variant complexes, F(1)-wt consisting of subunits alpha(3)beta(3)gammadeltaepsilon and F(1)Deltadelta lacking the entire delta-subunit as a prerequisite for overproduction and crystallization trials. Both F(1)-wt and F(1)Deltadelta were successfully overproduced in E. coli and purified in high yield and purity. F(1)Deltadelta was crystallized by micro-batch screening yielding three-dimensional crystals that diffracted to a resolution of 3.1A using a synchrotron radiation source. After establishing cryo and dehydrating conditions, a complete set of diffraction data was collected from a single crystal. No crystals were obtained with F(1)-wt. Data processing of diffraction patterns showed that F(1)Deltadelta crystals belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell parameters of a=121.70, b=174.80, and c=223.50A, alpha, beta, gamma=90.000. The asymmetric unit contained one molecule of bacterial F(1)Deltadelta with a corresponding volume per protein weight (V(M)) of 3.25A(3) Da(-1) and a solvent content of 62.1%. Silver staining of single crystals of F(1)Deltadelta analyzed by SDS-PAGE revealed four bands alpha, beta, gamma, and epsilon with identical M(r)-values as those found in the native F(1)F(0)-ATP synthase isolated from strain TA2.A1 membranes. ATPase assays of F(1)Deltadelta crystals exhibited latent ATP hydrolytic activity that was highly stimulated by lauryldimethylamine oxide, a hallmark of the native enzyme.

  12. Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna.

    Science.gov (United States)

    Mohamad Ishak, Nur Syafiqah; Kato, Yasuhiko; Matsuura, Tomoaki; Watanabe, Hajime

    2016-01-01

    Identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. Orthologs of Drosophila orphan receptor Fushi tarazu factor-1 (Ftz-F1) are known to function in genetic sex determination. In contrast, their roles in environmental sex determination remain unknown. In this study, we have cloned and characterized the Ftz-F1 ortholog in the branchiopod crustacean Daphnia magna, which produces males in response to environmental stimuli. Similar to that observed in Drosophila, D. magna Ftz-F1 (DapmaFtz-F1) produces two splicing variants, αFtz-F1 and βFtz-F1, which encode 699 and 777 amino acids, respectively. Both isoforms share a DNA-binding domain, a ligand-binding domain, and an AF-2 activation domain and differ only at the A/B domain. The phylogenetic position and genomic structure of DapmaFtz-F1 suggested that this gene has diverged from an ancestral gene common to branchiopod crustacean and insect Ftz-F1 genes. qRT-PCR showed that at the one cell and gastrulation stages, both DapmaFtz-F1 isoforms are two-fold more abundant in males than in females. In addition, in later stages, their sexual dimorphic expressions were maintained in spite of reduced expression. Time-lapse imaging of DapmaFtz-F1 RNAi embryos was performed in H2B-GFP expressing transgenic Daphnia, demonstrating that development of the RNAi embryos slowed down after the gastrulation stage and stopped at 30-48 h after ovulation. DapmaFtz-F1 shows high homology to insect Ftz-F1 orthologs based on its amino acid sequence and exon-intron organization. The sexually dimorphic expression of DapmaFtz-F1 suggests that it plays a role in environmental sex determination of D. magna.

  13. Hybrid breeding of Pampa-cytoplasmic triticale based on crosses with rye.

    Science.gov (United States)

    Łapiński, Bogusław

    2014-01-01

    The first series of field experiments with triticale F1 hybrids from 2009 confirmed usefulness of the rye 'Pampa' cytoplasmic system of mass crossing control in hexaploid (2n = 42 = AABBRR) winter triticale. The level of fertility restoration in 30 F1 hybrids varied within a range of 35.1% - 91.3%. However, the top F1 yield has not exceeded 92% of the check cultivar 'Moderato'. In the next series of field experiments with four winter F1 hybrids from 2012 the best yield reached 102% of the 'Moderato' standard and the fertility restoration index ranged between 16.7% and 100%. The top yielding F1 combination was produced using a male line derived from a cross with a restorer line from rye hybrid breeding. It supports the idea of using rye hybrid breeding as a source of variation in hybrid breeding of triticale. The advanced rye lines, representing well established complementary gene pools, show not only high potential for heterosis, but also high level of compensation for negative side effects of the 'Pampa' cytoplasm on plant vigor, which seems important in triticale. The gene flow from rye female and male gene pools to those of hexaploid triticale is facilitated with tetraploid triticale x diploid rye crosses. Numerous triploid F1 hybrids with relatively high fertility are produced, which are able to set seed after spontaneous pollination with hexaploid triticale.

  14. M2-F1 in flight during low-speed car tow

    Science.gov (United States)

    1963-01-01

    The M2-F1 shown in flight during a low-speed car tow runs across the lakebed. Such tests allowed about two minutes to test the vehicle's handling in flight. NASA Flight Research Center (later redesignated the Dryden Flight Research Center) personnel conducted as many as 8 to 14 ground-tow flights in a single day either to test the vehicle in preparation for air tows or to train pilots to fly the vehicle before they undertook air tows. The wingless, lifting body aircraft design was initially concieved as a means of landing an aircraft horizontally after atmospheric reentry. The absence of wings would make the extreme heat of re-entry less damaging to the vehicle. In 1962, Dryden management approved a program to build a lightweight, unpowered lifting body as a prototype to flight test the wingless concept. It would look like a 'flying bathtub,' and was designated the M2-F1, the 'M' referring to 'manned' and 'F' referring to 'flight' version. It featured a plywood shell placed over a tubular steel frame crafted at Dryden. Construction was completed in 1963. The first flight tests of the M2-F1 were over Rogers Dry Lake at the end of a tow rope attached to a hopped-up Pontiac convertible driven at speeds up to about 120 mph. This vehicle needed to be able to tow the M2-F1 on the Rogers Dry Lakebed adjacent to NASA's Flight Research Center (FRC) at a minimum speed of 100 miles per hour. To do that, it had to handle the 400-pound pull of the M2-F1. Walter 'Whitey' Whiteside, who was a retired Air Force maintenance officer working in the FRC's Flight Operations Division, was a dirt-bike rider and hot-rodder. Together with Boyden 'Bud' Bearce in the Procurement and Supply Branch of the FRC, Whitey acquired a Pontiac Catalina convertible with the largest engine available. He took the car to Bill Straup's renowned hot-rod shop near Long Beach for modification. With a special gearbox and racing slicks, the Pontiac could tow the 1,000-pound M2-F1 110 miles per hour in 30

  15. Recombinant bovine heart mitochondrial F1-ATPase inhibitor protein: overproduction in Escherichia coli, purification, and structural studies.

    Science.gov (United States)

    Van Heeke, G; Deforce, L; Schnizer, R A; Shaw, R; Couton, J M; Shaw, G; Song, P S; Schuster, S M

    1993-09-28

    A synthetic gene coding for the inhibitor protein of bovine heart mitochondrial F1 adenosine triphosphatase was designed and cloned in Escherichia coli. Recombinant F1-ATPase inhibitor protein was overproduced in E. coli and secreted to the periplasmic space. Biologically active recombinant F1-ATPase inhibitor protein was recovered from the bacterial cells by osmotic shock and was purified to near homogeneity in a single cation-exchange chromatography step. The recombinant inhibitor protein was shown to inhibit bovine mitochondrial F1-ATPase in a pH-dependent manner, as well as Saccharomyces cerevisiae mitochondrial F1-ATPase. Thorough analysis of the amino acid sequence revealed a potential coiled-coil structure for the C-terminal portion of the protein. Experimental evidence obtained by circular dichroism analyses supports this prediction and suggests F1I to be a highly stable, mainly alpha-helical protein which displays C-terminal alpha-helical coiled-coil intermolecular interaction.

  16. Meiotic analysis of interspecific hybrids between Capsicum frutescens and Capsicum chinense

    National Research Council Canada - National Science Library

    Nádia Fernandes Moreira; Telma Nair Santana Pereira; Kellen Coutinho Martins

    2017-01-01

    The aim of this study was to observe the genetic relationship between C. frutescens (UENF 1636) and C. chinense (UENF 1785) based on the meiotic behavior, on the meiotic index, and on pollen viability of their F1 hybrids...

  17. Interspecific hybridization between greater kudu and nyala.

    Science.gov (United States)

    Dalton, Desiré L; Tordiffe, Adrian; Luther, Ilse; Duran, Assumpta; van Wyk, Anna M; Brettschneider, Helene; Oosthuizen, Almero; Modiba, Catherine; Kotzé, Antoinette

    2014-06-01

    Hybridization of wildlife species, even in the absence of introgression, is of concern due to wasted reproductive effort and a reduction in productivity. In this study we detail an accidental mating between a female nyala (Tragelaphus angasii) and a male greater kudu (T. strepsiceros). The hybrid was phenotypically nyala and was identified as such based on mitochondrial DNA. Further genetic analysis based on nine microsatellite markers, chromosome number and chromosome morphology however, confirmed its status as an F1 hybrid. Results obtained from a reproductive potential assessment indicated that this animal does not have the potential to breed successfully and can be considered as sterile.

  18. Armazenamento sob condições ambiente e aceitabilidade do melão 'F1 Jangada' produzido em sistema hidropônico Storage under atmosphere conditions and acceptability of the melon 'F1 Jangada' croped in hydroponic system

    Directory of Open Access Journals (Sweden)

    Maria M. Rinaldi

    2006-12-01

    Full Text Available O objetivo deste trabalho foi determinar o período de armazenamento pós-colheita e a aceitabilidade pelo consumidor de melão híbrido 'F1 Jangada' (Cucumis melo L., produzido em sistema hidropônico, mantido em condições ambiente (22 ± 2 ºC e umidade relativa de 40 ± 5%. O experimento compreendeu o período de 21-6-2005 a 2-8-2005. Foi utilizado o esquema fatorial 5 x 2, em delineamento experimental inteiramente casualizado, com cinco períodos de armazenamento (0; 7; 21; 28 e 42 dias e dois tipos de substrato (areia e fibra de coco, com três repetições, em que cada repetição consistiu em cinco frutos de meloeiro. Foram avaliados o pH, a acidez titulável, os sólidos solúveis, a perda de massa fresca, a análise sensorial e a decisão de compra dos melões. Foram verificados efeitos do tipo de substrato e tempo de armazenamento sobre os valores de pH dos melões. A acidez titulável dos melões diminuiu significativamente nos primeiros sete dias de armazenamento, em ambos os substratos. Não foram verificados efeitos do tipo de substrato e tempo de armazenamento nos sólidos solúveis dos melões durante o armazenamento. Não houve diferença de perda de massa fresca dos frutos produzidos nos dois substratos, sendo de 7,1 ± 0,2%, durante os 42 dias de armazenamento. O tipo de substrato não interferiu na aparência geral, cor, textura e sabor dos melões. Aos 42 dias de armazenamento, os melões produzidos nos dois tipos de substrato apresentaram-se aceitáveis pelo consumidor. No entanto, os produzidos no substrato com areia apresentaram melhor aceitabilidade e decisão de compra ao longo do armazenamento.The objective of this work was to evaluate the storage period postharvest and acceptability by consumer of hybrid melon 'F1 Jangada' (Cucumis melo L., produced in hydroponic system, stored in atmosphere conditions (22 ± 2 ºC and 40 ± 5% relative humidity. The research was carried from June 21st to August 2nd, 2005. It was

  19. E2F1 and p53 Transcription Factors as Accessory Factors for Nucleotide Excision Repair

    Directory of Open Access Journals (Sweden)

    David G. Johnson

    2012-10-01

    Full Text Available Many of the biochemical details of nucleotide excision repair (NER have been established using purified proteins and DNA substrates. In cells however, DNA is tightly packaged around histones and other chromatin-associated proteins, which can be an obstacle to efficient repair. Several cooperating mechanisms enhance the efficiency of NER by altering chromatin structure. Interestingly, many of the players involved in modifying chromatin at sites of DNA damage were originally identified as regulators of transcription. These include ATP-dependent chromatin remodelers, histone modifying enzymes and several transcription factors. The p53 and E2F1 transcription factors are well known for their abilities to regulate gene expression in response to DNA damage. This review will highlight the underappreciated, transcription-independent functions of p53 and E2F1 in modifying chromatin structure in response to DNA damage to promote global NER.

  20. Proximity effects in superconducting triplet spin-valve F2/F1/S

    Energy Technology Data Exchange (ETDEWEB)

    Deminov, R.G., E-mail: Raphael.Deminov@kpfu.ru [Institute of Physics, Kazan Federal University, Kazan 420008 (Russian Federation); Tagirov, L.R. [Institute of Physics, Kazan Federal University, Kazan 420008 (Russian Federation); Institut für Physik, Universität Augsburg, Augsburg D-86159 (Germany); Gaifullin, R.R. [Institute of Physics, Kazan Federal University, Kazan 420008 (Russian Federation); Karminskaya, T.Yu.; Kupriyanov, M.Yu. [Skobeltsyn Institute of Nuclear Physics, Moscow State University, Moscow 119992 (Russian Federation); Fominov, Ya.V. [Landau Institute for Theoretical Physics RAS, Moscow 119334 (Russian Federation); Golubov, A.A. [Faculty of Science and Technology and MESA+ Institute of Nanotechnology, University of Twente, P.O. Box 217, Enschede 7500 AE (Netherlands)

    2015-01-01

    We investigate the critical temperature T{sub c} of F2/F1/S trilayers (Fi is a ferromagnetic metal and S is a singlet superconductor), where the long-range triplet superconducting component is generated at noncollinear magnetizations of the F layers. In this paper we demonstrate a possibility of the spin-valve effect mode selection (standard switching effect, the triplet spin-valve effect or reentrant T{sub c}(α) dependence) by the variation of the F2/F1 interface transparency. - Highlights: • T{sub c} of FFS trilayer as a function of angle between magnetizations is calculated. • T{sub c} of FFS structure for arbitrary FF interface transparencies γ{sub B} is calculated. • Possibility of the spin-valve effect mode selection by the variation of γ{sub B} is shown.

  1. Purification and Activity of Antibacterial Substances Derived from Soil Streptomyces sp.CaiF1

    Institute of Scientific and Technical Information of China (English)

    Hui YANG; Guixiang PENG; Jianmin ZENG; Zhiyuan TAN

    2012-01-01

    [Objective] This study aimed to separate and purify antibacterial sub- stances from soil Streptomyces sp. CaiF1, and to explore the activities of this sub- stance. [Method] The antibacterial substances were separated and purified by Ethyl acetate extraction, macroporous adsorptive resin, silica gel chromatography and preparative high performance liquid chromatography (HPLC), and powdery mildew were taken as the indicating bacterial to study their activities. [Result] Antibacterial substances were purified and the stability analysis of the extracts from Streptomyces CaiF1 fermentation broth showed very stable at pH 2.0-pH 10.0, 100 ~C and changed very little under UV treatment for 24 h. Inhibition rate of powdery mildew was 69.7%. [Conclusion] The purified antibacterial substances showed good stability, which provided theoretical foundation for their structural identifications and future ap- plications.

  2. Oral vaccination with salmonella simultaneously expressing Yersinia pestis F1 and V antigens protects against bubonic and pneumonic plague.

    Science.gov (United States)

    Yang, Xinghong; Hinnebusch, B Joseph; Trunkle, Theresa; Bosio, Catharine M; Suo, Zhiyong; Tighe, Mike; Harmsen, Ann; Becker, Todd; Crist, Kathryn; Walters, Nancy; Avci, Recep; Pascual, David W

    2007-01-15

    The gut provides a large area for immunization enabling the development of mucosal and systemic Ab responses. To test whether the protective Ags to Yersinia pestis can be orally delivered, the Y. pestis caf1 operon, encoding the F1-Ag and virulence Ag (V-Ag) were cloned into attenuated Salmonella vaccine vectors. F1-Ag expression was controlled under a promoter from the caf1 operon; two different promoters (P), PtetA in pV3, PphoP in pV4, as well as a chimera of the two in pV55 were tested. F1-Ag was amply expressed; the chimera in the pV55 showed the best V-Ag expression. Oral immunization with Salmonella-F1 elicited elevated secretory (S)-IgA and serum IgG titers, and Salmonella-V-Ag(pV55) elicited much greater S-IgA and serum IgG Ab titers than Salmonella-V-Ag(pV3) or Salmonella-V-Ag(pV4). Hence, a new Salmonella vaccine, Salmonella-(F1+V)Ags, made with a single plasmid containing the caf1 operon and the chimeric promoter for V-Ag allowed the simultaneous expression of F1 capsule and V-Ag. Salmonella-(F1+V)Ags elicited elevated Ab titers similar to their monotypic derivatives. For bubonic plague, mice dosed with Salmonella-(F1+V)Ags and Salmonella-F1-Ag showed similar efficacy (>83% survival) against approximately 1000 LD(50) Y. pestis. For pneumonic plague, immunized mice required immunity to both F1- and V-Ags because the mice vaccinated with Salmonella-(F1+V)Ags protected against 100 LD(50) Y. pestis. These results show that a single Salmonella vaccine can deliver both F1- and V-Ags to effect both systemic and mucosal immune protection against Y. pestis.

  3. Direct analysis of airborne mite allergen (Der f1) in the residential atmosphere by chemifluorescent immunoassay using bioaerosol sampler.

    Science.gov (United States)

    Miyajima, Kumiko; Suzuki, Yurika; Miki, Daisuke; Arai, Moeka; Arakawa, Takahiro; Shimomura, Hiroji; Shiba, Kiyoko; Mitsubayashi, Kohji

    2014-06-01

    Dermatophagoides farinae allergen (Der f1) is one of the most important indoor allergens associated with allergic diseases in humans. Mite allergen Der f1 is usually associated with particles of high molecular weight; thus, Der f1 is generally present in settled dust. However, a small quantity of Der f1 can be aerosolized and become an airborne component. Until now, a reliable method of detecting airborne Der f1 has not been developed. The aim of this study was to develop a fiber-optic chemifluorescent immunoassay for the detection of airborne Der f1. In this method, the Der f1 concentration measured on the basis of the intensity of fluorescence amplified by an enzymatic reaction between the labeled enzyme by a detection antibody and a fluorescent substrate. The measured Der f1 concentration was in the range from 0.49 to 250 ng/ml and a similar range was found by enzyme-linked immunosorbent assay (ELISA). This method was proved to be highly sensitive to Der f1 compared with other airborne allergens. For the implementation of airborne allergen measurement in a residential environment, a bioaerosol sampler was constructed. The airborne allergen generated by a nebulizer was conveyed to a newly sampler we developed for collecting airborne Der f1. The sampler was composed of polymethyl methacrylate (PMMA) cells for gas/liquid phases and some porous membranes which were sandwiched in between the two phases. Der f1 in air was collected by the sampler and measured using the fiber-optic immunoassay system. The concentration of Der f1 in aerosolized standards was in the range from 0.125 to 2.0 mg/m(3) and the collection rate of the device was approximately 0.2%.

  4. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation

    DEFF Research Database (Denmark)

    Helin, K; Wu, C L; Fattaey, A R

    1993-01-01

    homolog of DP-1. Human DP-1 and E2F-1 associate both in vivo and in vitro, and this interaction leads to enhanced binding to E2F DNA-binding sites. The association of E2F-1 and DP-1 leads to cooperative activation of an E2F-responsive promoter. Finally, we demonstrate that E2F-1 and DP-1 association...

  5. Agraphia and acalculia after a left prefrontal (F1, F2) infarction.

    OpenAIRE

    Tohgi, H; Saitoh, K.; S. Takahashi(Kobe University, J-657-8501 Kobe, Japan); Takahashi, H; Utsugisawa, K; Yonezawa, H.; Hatano, K.; Sasaki, T.

    1995-01-01

    A patient presented with agraphia and acalculia associated with a left frontal (F1, F2) infarction. He made mainly phonological but also lexical errors in writing (syllabograms), but his ability to write kanji (morphograms) was relatively preserved. Although he could add and subtract numbers, he could neither multiply nor divide them because of a difficulty in retrieving the multiplication tables and calculation procedures. Positron emission tomography showed decreased cerebral blood flow and...

  6. VIIRS F1 "best" relative spectral response characterization by the government team

    Science.gov (United States)

    Moeller, Chris; McIntire, Jeff; Schwarting, Tom; Moyer, Dave

    2011-10-01

    The VIIRS Flight 1 (F1) instrument completed sensor level testing, including relative spectral response (RSR) characterization in 2009 and is moving forward towards a launch on the NPP platform late in 2011. As part of its mandate to produce analyses of F1 performance essentials, the VIIRS Government Team, consisting of NASA, Aerospace Corp., and MIT/Lincoln Lab elements, has produced an independent (from that of industry) analysis of F1 RSR. The test data used to derive RSR for all VIIRS spectral bands was collected in the TVAC environment using the Spectral Measurement Assembly (SpMA), a dual monochromator system with tungsten and ceramic glow bar sources. These spectrally contiguous measurements were analyzed by the Government Team to produce a complete in-band + out-of-band RSR for 21 of the 22 VIIRS bands (exception of the Day-Night Band). The analysis shows that VIIRS RSR was well measured in the pre-launch test program for all bands, although the measurement noise floor is high on the thermal imager band I5. The RSR contain expected detector to detector variation resulting from the VIIRS non-telecentric optical design, and out-of-band features are present in some bands; non-compliances on the integrated out-of-band spectral performance metric are noted in M15 and M16A,B bands and also for several VisNIR bands, though the VisNIR non-compliances were expected due to known scattering in the VisNIR integrated filter assembly. The Government Team "best" RSR have been released into the public domain for use by the science community in preparation for the post-launch era of VIIRS F1.

  7. Mitogenic Sonic hedgehog signaling drives E2F1-dependent lipogenesis in progenitor cells and Medulloblastoma

    OpenAIRE

    Bhatia, Bobby; Hsieh, Michael; Kenney, Anna Marie; Nahlé, Zaher

    2010-01-01

    Deregulation of the Rb/E2F tumor suppressor complex and aberrantion of Sonic hedgehog (Shh) signaling are documented across the spectrum of human malignancies. Exaggerated de novo lipid synthesis is also found in certain highly proliferative, aggressive tumors. Here, we show that in Shh-driven medulloblastomas, Rb is inactivated and E2F1 is up-regulated, promoting lipogenesis. Extensive lipid accumulation and elevated levels of the lipogenic enzyme FASN mark those tumors. In primary cerebella...

  8. Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.

    OpenAIRE

    Zylstra, G J; Wackett, L P; Gibson, D T

    1989-01-01

    Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroethylene degradation by the recombinant organism depended on the cell concentration and the concentration of trichloroethylene. A linear rate of trichloroethylene degradation was o...

  9. Studies of carcinogenicity of sodium chlorite in B6C3F1 mice.

    Science.gov (United States)

    Yokose, Y; Uchida, K; Nakae, D; Shiraiwa, K; Yamamoto, K; Konishi, Y

    1987-01-01

    The carcinogenic activities of sodium chlorite in B6C3F1 mice were examined. Sodium chlorite was given at concentrations of 0 (control), 0.025% (low dose), or 0.05% (high dose) in the drinking water of 150 female and 150 male mice for 80 weeks, after which time the animals were returned to distilled water without sodium chlorite. All mice were sacrificed 85 weeks from the beginning of the experiment. The incidence of tumor-bearing animals was 32% (control), 34% (low dose), and 26% (high dose) in female mice, and 46% (control), 57% (low dose), and 53% (high dose) in male mice. The types and incidence of neoplasms that occurred frequently in each group of both sexes were similar to those observed spontaneously in B6C3F1 mice. The incidence of lymphomas/leukemias in the high dose group of females (2%), however, was lower than that in the control group (15%). Furthermore, the incidence of pulmonary adenomas in the high dose group of males (12%) was higher than that in the control group (0%), but neither dose-related increases in the adenoma incidences nor increased incidences of the adenocarcinomas were observed. These results indicated no clear evidence of a carcinogenic potential of sodium chlorite in B6C3F1 mice. PMID:3447900

  10. Depigmenting Effect of Kojic Acid Esters in Hyperpigmented B16F1 Melanoma Cells

    Science.gov (United States)

    Lajis, Ahmad Firdaus B.; Hamid, Muhajir; Ariff, Arbakariya B.

    2012-01-01

    The depigmenting effect of kojic acid esters synthesized by the esterification of kojic acid using Rhizomucor miehei immobilized lipase was investigated in B16F1 melanoma cells. The depigmenting effect of kojic acid and kojic acid esters was evaluated by the inhibitory effect of melanin formation and tyrosinase activity on alpha-stimulating hormone- (α-MSH-) induced melanin synthesis in B16F1 melanoma cells. The cellular tyrosinase inhibitory effect of kojic acid monooleate, kojic acid monolaurate, and kojic acid monopalmitate was found similar to kojic acid at nontoxic doses ranging from 1.95 to 62.5 μg/mL. However, kojic acid monopalmitate gave slightly higher inhibition to melanin formation compared to other inhibitors at doses ranging from 15.63 to 62.5 μg/mL. Kojic acid and kojic acid esters also show antioxidant activity that will enhance the depigmenting effect. The cytotoxicity of kojic acid esters in B16F1 melanoma cells was significantly lower than kojic acid at high doses, ranging from 125 and 500 μg/mL. Since kojic acid esters have lower cytotoxic effect than kojic acid, it is suggested that kojic acid esters can be used as alternatives for a safe skin whitening agent and potential depigmenting agents to treat hyperpigmentation. PMID:23091364

  11. Properties of kojic acid and curcumin: Assay on cell B16-F1

    Science.gov (United States)

    Sugiharto, Ariff, Arbakariya; Ahmad, Syahida; Hamid, Muhajir

    2016-03-01

    Ultra violet (UV) exposure and oxidative stress are casually linked to skin disorders. They can increase melanin synthesis, proliferation of melanocytes, and hyperpigmentation. It is possible that antioxidants or inhibitors may have a beneficial effect on skin health to reduce hyperpigmentation. In the last few years, a huge number of natural herbal extracts have been tested to reduce hyperpigmentation. The objective of this study was to determine and to compare of kojic acid and curcumin properties to viability cell B16-F1. In this study, our data showed that the viability of cell B16-F1 was 63.91% for kojic acid and 64.12% for curcumin at concentration 100 µg/ml. Further investigation assay of antioxidant activities, indicated that IC50 for kojic acid is 63.8 µg/ml and curcumin is 16.05 µg/ml. Based on the data, kojic acid and curcumin have potential antioxidant properties to reduce hyperpigmentation with low toxicity effect in cell B16-F1.

  12. Quaternary structure of V1 and F1 ATPase: significance of structural homologies and diversities.

    Science.gov (United States)

    Svergun, D I; Konrad, S; Huss, M; Koch, M H; Wieczorek, H; Altendorf, K; Volkov, V V; Grüber, G

    1998-12-22

    The V1 ATPase from the tobacco hornworm Manduca sexta and the Escherichia coli F1 ATPase were characterized by small-angle X-ray scattering (SAXS). The radii of gyration (Rg) of the complexes were 6.2 +/- 0.1 and 4.7 +/- 0.02 nm, respectively. The shape of the M. sexta V1 ATPase was determined ab initio from the scattering data showing six masses, presumed to be the A and B subunits, arranged in an alternating manner about a 3-fold axis. A seventh mass with a length of about 11.0 nm extends perpendicularly to the center of the hexameric unit. This central mass is presumed to be the stalk that connects V1 with the membrane domain (V(O)) in the intact V1V(O)-ATPase. In comparison, the shape of the F1 ATPase from E. coli possesses a quasi-3-fold symmetry over the major part of the enzyme. The overall asymmetry of the structure is given by a stem, assumed to include the central stalk subunits. The features of the V1 and F1 ATPase reveal structural homologies and diversities of the key components of the complexes.

  13. Complementation of Escherichia coli unc mutant strains by chloroplast and cyanobacterial F1-ATPase subunits.

    Science.gov (United States)

    Lill, H; Burkovski, A; Altendorf, K; Junge, W; Engelbrecht, S

    1993-10-04

    The genes encoding the five subunits of the F1 portion of the ATPases from both spinach chloroplasts and the cyanobacterium Synechocystis sp. PCC 6803 were cloned into expression vectors and expressed in Escherichia coli. The recombinant subunits formed inclusion bodies within the cells. Each particular subunit was expressed in the respective unc mutant, each unable to grow on non-fermentable carbon sources. The following subunits restored growth under conditions of oxidative phosphorylation: alpha (both sources, cyanobacterial subunit more than spinach subunit), beta (cyanobacterial subunit only), delta (both spinach and Synechocystis), and epsilon (both sources), whereas no growth was achieved with the gamma subunits from both sources. Despite a high degree of sequence homology the large subunits alpha and beta of spinach and cyanobacterial F1 were not as effective in the substitution of their E. coli counterparts. On the other hand, the two smallest subunits of the E. coli ATPase could be more effectively replaced by their cyanobacterial or chloroplast counterparts, although the sequence identity or even similarity is very low. We attribute these findings to the different roles of these subunits in F1: The large alpha and beta subunits contribute to the catalytic centers of the enzyme, a function rendering them very sensitive to even minor changes. For the smaller delta and epsilon subunits it was sufficient to maintain a certain tertiary structure during evolution, with little emphasis on the conservation of particular amino acids.

  14. Depigmenting Effect of Kojic Acid Esters in Hyperpigmented B16F1 Melanoma Cells

    Directory of Open Access Journals (Sweden)

    Ahmad Firdaus B. Lajis

    2012-01-01

    Full Text Available The depigmenting effect of kojic acid esters synthesized by the esterification of kojic acid using Rhizomucor miehei immobilized lipase was investigated in B16F1 melanoma cells. The depigmenting effect of kojic acid and kojic acid esters was evaluated by the inhibitory effect of melanin formation and tyrosinase activity on alpha-stimulating hormone- (α-MSH- induced melanin synthesis in B16F1 melanoma cells. The cellular tyrosinase inhibitory effect of kojic acid monooleate, kojic acid monolaurate, and kojic acid monopalmitate was found similar to kojic acid at nontoxic doses ranging from 1.95 to 62.5 μg/mL. However, kojic acid monopalmitate gave slightly higher inhibition to melanin formation compared to other inhibitors at doses ranging from 15.63 to 62.5 μg/mL. Kojic acid and kojic acid esters also show antioxidant activity that will enhance the depigmenting effect. The cytotoxicity of kojic acid esters in B16F1 melanoma cells was significantly lower than kojic acid at high doses, ranging from 125 and 500 μg/mL. Since kojic acid esters have lower cytotoxic effect than kojic acid, it is suggested that kojic acid esters can be used as alternatives for a safe skin whitening agent and potential depigmenting agents to treat hyperpigmentation.

  15. Somatic development and embryo yield in crossbred F1 mice generated by different mating strategies

    Directory of Open Access Journals (Sweden)

    RITP. Batista

    Full Text Available The aim of this study was to evaluate different mating strategies among endogamic strains to create F1 populations of mice, minimising the effect of inbreeding depression on somatic development and embryo yield. Females from the strains Swiss, CBA and C57Bl/6 were divided in nine experimental mate arrangements. The total numbers of pups born alive per dam and somatic development, estimated by weighing and measuring the crown-rump length, were recorded. Superovulation response was evaluated in outbreed females. Litter size differed among endogamic dams, irrespective of the sire. Somatic development results suggest heterosis and imprinting phenomena, once a differential parental effect was demonstrated. There was no difference in corpora lutea, ova or embryos recovered (P > 0.05, but recovery and viability rates differ among F1 groups (P < 0.05. The association of dam prolificity with somatic development and superovulation response of the pups should be considered for experimental F1 populations establishment. The use of outbreed animals, however, did not reduce response variability to hormone treatment.

  16. Psychoacoustical and ear canal cancellation of (2f1-f2)-distortion products.

    Science.gov (United States)

    Zwicker, E; Harris, F P

    1990-06-01

    Level and phase of the (2f1-f2)-difference tone were measured as a function of primary-tone level using the psychoacoustical method of cancellation and the objective method of emission cancellation for four frequency separations of f1 = 1620 Hz and f2 in four subjects. Differences between hearing- and emission-cancellation levels ranged from 60-33 dB as delta f = f2-f1 increased from 180 to 432 Hz. For smaller separations of the primaries, phase changes for emission cancellation covered a wide range and had sharp "steps," whereas for hearing cancellation, the phase varied only slightly. With wider separations of the primaries, the phase became more varied for hearing cancellation and more homogeneous for emission cancellation. Both emission- and hearing-cancellation level functions were nonmonotonic as a function of constant SL1 and varied SL2. Remarkable phase shifts always appeared near minima in level at all separations of the primaries for emission cancellation. Four sources may be contributing to the differences in results: (a) the frequency-dependent attenuation of the middle-ear transfer function, (b) the frequency-dependent mismatch of the acoustical impedances at the eardrum, (c) the frequency dependence of the microphone's sensitivity mounted within the probe, and (d) the different reaction of active nonlinear cochlear processes on the hearing- and emission-cancellation tones.

  17. Nanosized free-energy transducer F1-ATPase achieves 100% efficiency at finite time operation

    CERN Document Server

    Toyabe, Shoichi

    2012-01-01

    The free-energy transduction at 100% efficiency is not prohibited by thermodynamic laws. However, it is usually reached only at the quasi-static limit such as the macroscopic piston pulled or pushed at the infinitely slow velocity. If we operate the piston quickly, turbulence is inevitable and irreversible heat dissipates through the microscopic degrees of freedom. Here, we evaluated the work performed by the nano-sized biological free-energy transducer F1-ATPase by single-molecule experiments on the basis of nonequilibrium theory. We show that the F1-ATPase achieves a nearly 100% free-energy conversion efficiency even far from quasistatic process for both the mechanical-to-chemical and chemical-to-mechanical transductions. Such a high efficiency at a finite-time operation is not expected for macroscopic engines and highlights a remarkable property of the nano-sized engines working in the energy scale of k_{B}T. Some of the microscopic degrees of freedom may not be hidden but accessible to the F1-ATPase. Henc...

  18. Simultaneous F 0-F 1 modifications of Arabic for the improvement of natural-sounding

    Science.gov (United States)

    Ykhlef, F.; Bensebti, M.

    2013-03-01

    Pitch (F 0) modification is one of the most important problems in the area of speech synthesis. Several techniques have been developed in the literature to achieve this goal. The main restrictions of these techniques are in the modification range and the synthesised speech quality, intelligibility and naturalness. The control of formants in a spoken language can significantly improve the naturalness of the synthesised speech. This improvement is mainly dependent on the control of the first formant (F 1). Inspired by this observation, this article proposes a new approach that modifies both F 0 and F 1 of Arabic voiced sounds in order to improve the naturalness of the pitch shifted speech. The developed strategy takes a parallel processing approach, in which the analysis segments are decomposed into sub-bands in the wavelet domain, modified in the desired sub-band by using a resampling technique and reconstructed without affecting the remained sub-bands. Pitch marking and voicing detection are performed in the frequency decomposition step based on the comparison of the multi-level approximation and detail signals. The performance of the proposed technique is evaluated by listening tests and compared to the pitch synchronous overlap and add (PSOLA) technique in the third approximation level. Experimental results have shown that the manipulation in the wavelet domain of F 0 in conjunction with F 1 guarantees natural-sounding of the synthesised speech compared to the classical pitch modification technique. This improvement was appropriate for high pitch modifications.

  19. Bioleaching of heavy metals from a contaminated soil using indigenous Penicillium chrysogenum strain F1.

    Science.gov (United States)

    Deng, Xinhui; Chai, Liyuan; Yang, Zhihui; Tang, Chongjian; Tong, Haixia; Yuan, Pingfu

    2012-09-30

    Bioleaching of heavy metals from contaminated soil using Penicillium chrysogenum strain F1 was investigated. Batch experiments were performed to compare leaching efficiencies of heavy metals between one-step and two-step processes and to determine the transformation of heavy metal fractions before and after bioleaching. The results showed that two-step process had higher leaching efficiencies of heavy metals than one-step process. When the mass ratio of soil to culture medium containing P. chrysogenum strain F1 was 5% (w/v), 50%, 35%, 9% and 40% of Cd, Cu, Pb and Zn were removed in one-step process, respectively. The two-step process had higher removals of 63% Cd, 56% Cu, 14% Pb and 54% Zn as compared with one-step process. The results of the sequential extraction showed that the metals remaining in the soil were mainly bonded in stable fractions after bioleaching. The results of TEM and SEM showed that during bioleaching process, although the mycelium of P. chrysogenum was broken into fragments, no damage was obviously observed on the surface of the living cell except for thinner cell wall, smaller vacuoles and concentrated cytoplasm. The result implied that P. chrysogenum strain F1 can be used to remove heavy metals from polluted soil.

  20. F0F1-ATP-Synthase aus Escherichia coli: Untersuchung verschiedener Proteinsysteme mit ESR-Spektroskopie

    OpenAIRE

    Kraft, Gerhard

    1999-01-01

    Das Enzym F0F1-ATP-Synthase katalysiert die Phosphorylierung von ADP zu ATP unter Ausnutzung des durch die Atmungskette entstehenden Protonengradienten an Membranen. Hierbei pumpt der membranintegrale F0-Teil des Proteins Protonen durch die Membran und induziert die ATP-Synthese, welche auf dem peripheren, wasserlöslichen F1-Teil des Proteins (F1-ATPase) stattfindet. F0 besteht aus drei Proteinuntereinheiten der Stöchiometrie a b_2 c_9-12, während F1 aus fünf Untereinheiten der Stöchiometrie ...

  1. Hybrid Baryons

    CERN Document Server

    Page, P R

    2003-01-01

    We review the status of hybrid baryons. The only known way to study hybrids rigorously is via excited adiabatic potentials. Hybrids can be modelled by both the bag and flux-tube models. The low-lying hybrid baryon is N 1/2^+ with a mass of 1.5-1.8 GeV. Hybrid baryons can be produced in the glue-rich processes of diffractive gamma N and pi N production, Psi decays and p pbar annihilation.

  2. The F1-F2 vowel chart for Czech whispered vowels a, e, i, o, u.

    Science.gov (United States)

    Grepl, Michal; Furst, Tomas; Pesak, Josef

    2007-12-01

    The aim of this contribution is to present the formant chart of the Czech vowels a, e, i, o, u and show that this can be achieved by means of digital methods of sound processing. A group of 35 Czech students of the Pedagogical Faculty of Palacky University was tested and a record of whispered vowels was taken from each of them. The record was digitalized and processed by the Discrete Fourier Trasform. The result is the power spectrum of the individual vocals - the graphic output consists of a plot of the relative power of individual frequencies in the original sound. The values of the first two maxima which represent the first and the second formants were determined from the graph. The values were plotted on a formant chart. Altogether, 175 spectral analyses of individual vowels were performed. In the resulting power spectrum, the first and the second formant frequencies were identified. The first formant was plotted against the second one and pure vocal formant regions were identified. Frequency bands for the Czech vowel "a" were circumscribed between 850 and 1150 Hz for first formant (F1) and between 1200 and 2000 Hz for second formant (F2). Similarly, borders of frequency band for vowel "e" they were 700 and 950 Hz for F1 and 1700 and 3000 Hz for F2. For vowel "i" 300 and 450 Hz for F1 and 2000 and 3600 Hz for F2, for vowel "o" 600 and 800 Hz for F1 and 600 and 1400 Hz for F2, for vowel "u" 100 and 400 Hz for F1 and 400 and 1200 Hz for F2. At low frequencies it is feasible to invoke the source-filter model of voice production and associate vowel identity with frequencies of the first two formants in the voice spectrum. On the other hand, subject to intonation, singing or other forms of exposed voice (such as emotional speech, focused speech), the formant regions tend to spread. In spectral analysis other frequencies dominate, so specific formant frequency bands are not easily recognizable. Although the resulting formant map is not much different from the formant

  3. Mapping of Hd6-f1 Gene on Rice Heading Date%水稻抽穗期基因 Hd6-f1的定位

    Institute of Scientific and Technical Information of China (English)

    周鹏飞; 柳絮; 张华; 宣宁; 李军; 杨永义; 李广贤; 姚方印; 刘开启

    2015-01-01

    本试验以明恢63(供体)/早熟豫6(受体)的BC4 F2分离群体为材料,采用BSA ( Bulked segrega-tion analysis)法,对控制水稻抽穗期分离的基因进行SSR分子标记定位。 BC4 F2出现抽穗期分离,早抽穗植株为253株,晚抽穗植株为657株,χ2=3.66<P0.05=3.84,符合1∶3的孟德尔分离比,表明F2群体的抽穗期分离受一对等位基因控制,晚抽穗性状为显性。以明恢63×早熟豫6的BC4 F2分离群体的253株隐性单株为定位群体,将该抽穗基因(暂时命名为Hd6-f1)定位在水稻第6染色体分子标记RM19771与RM527之间,遗传距离分别为0.2 cM和2.9 cM,与RM19780共分离。%Using the BC4F2 segregation populations of Minghui 63(donor) and Zaoshuyu 6(receptor) as materials, the SSR markers were located for the gene on rice heading date by the bulked segregation analysis (BSA).The BC4F2 population had 253 early heading plants and 657 late heading plants( χ2 =3.66f1 was mapped on chromosome 6 between SSR markers RM19771 and RM527 with the genetic distances as 0.2 cM and 2.9 cM respectively, and co-segregated with RM19780 .

  4. Mechanistic basis of infertility of mouse intersubspecific hybrids.

    Science.gov (United States)

    Bhattacharyya, Tanmoy; Gregorova, Sona; Mihola, Ondrej; Anger, Martin; Sebestova, Jaroslava; Denny, Paul; Simecek, Petr; Forejt, Jiri

    2013-02-01

    According to the Dobzhansky-Muller model, hybrid sterility is a consequence of the independent evolution of related taxa resulting in incompatible genomic interactions of their hybrids. The model implies that the incompatibilities evolve randomly, unless a particular gene or nongenic sequence diverges much faster than the rest of the genome. Here we propose that asynapsis of heterospecific chromosomes in meiotic prophase provides a recurrently evolving trigger for the meiotic arrest of interspecific F1 hybrids. We observed extensive asynapsis of chromosomes and disturbance of the sex body in >95% of pachynemas of Mus m. musculus × Mus m. domesticus sterile F1 males. Asynapsis was not preceded by a failure of double-strand break induction, and the rate of meiotic crossing over was not affected in synapsed chromosomes. DNA double-strand break repair was delayed or failed in unsynapsed autosomes, and misexpression of chromosome X and chromosome Y genes was detected in single pachynemas and by genome-wide expression profiling. Oocytes of F1 hybrid females showed the same kind of synaptic problems but with the incidence reduced to half. Most of the oocytes with pachytene asynapsis were eliminated before birth. We propose the heterospecific pairing of homologous chromosomes as a preexisting condition of asynapsis in interspecific hybrids. The asynapsis may represent a universal mechanistic basis of F1 hybrid sterility manifested by pachytene arrest. It is tempting to speculate that a fast-evolving subset of the noncoding genomic sequence important for chromosome pairing and synapsis may be the culprit.

  5. 牛、羊POU1F1基因的研究进展%The Advanced Progress of POU1F1 Gene in Bovine and Caprine

    Institute of Scientific and Technical Information of China (English)

    胡志焕

    2008-01-01

    POU1F1基因是 POU 基因家族的重要成员.POU1F1蛋白在哺乳动物胚胎分化和生长发育过程中起着关键作用.本文综述POU1F1基因的结构特征与功能,POU1F1蛋白的结构特征与作用方式,POU1F1蛋白的调控机制以及近年来对牛、羊POUlFl基因遗传变异及其与经济性状关系研究的最新进展.

  6. Speciation and reduced hybrid female fertility in house mice.

    Science.gov (United States)

    Suzuki, Taichi A; Nachman, Michael W

    2015-09-01

    In mammals, intrinsic postzygotic isolation has been well studied in males but has been less studied in females, despite the fact that female gametogenesis and pregnancy provide arenas for hybrid sterility or inviability that are absent in males. Here, we asked whether inviability or sterility is observed in female hybrids of Mus musculus domesticus and M. m. musculus, taxa which hybridize in nature and for which male sterility has been well characterized. We looked for parent-of-origin growth phenotypes by measuring adult body weights in F1 hybrids. We evaluated hybrid female fertility by crossing F1 females to a tester male and comparing multiple reproductive parameters between intrasubspecific controls and intersubspecific hybrids. Hybrid females showed no evidence of parent-of-origin overgrowth or undergrowth, providing no evidence for reduced viability. However, hybrid females had smaller litter sizes, reduced embryo survival, fewer ovulations, and fewer small follicles relative to controls. Significant variation in reproductive parameters was seen among different hybrid genotypes, suggesting that hybrid incompatibilities are polymorphic within subspecies. Differences in reproductive phenotypes in reciprocal genotypes were observed and are consistent with cyto-nuclear incompatibilities or incompatibilities involving genomic imprinting. These findings highlight the potential importance of reduced hybrid female fertility in the early stages of speciation.

  7. Gonad morphogenesis defects drive hybrid male sterility in asymmetric hybrid breakdown of Caenorhabditis nematodes.

    Science.gov (United States)

    Dey, Alivia; Jin, Qi; Chen, Yen-Chu; Cutter, Asher D

    2014-01-01

    Determining the causes and evolution of reproductive barriers to gene flow between populations, speciation, is the key to understanding the origin of diversity in nature. Many species manifest hybrid breakdown when they intercross, characterized by increasingly exacerbated problems in later generations of hybrids. Recently, Caenorhabditis nematodes have emerged as a genetic model for studying speciation, and here we investigate the nature and causes of hybrid breakdown between Caenorhabditis remanei and C. latens. We quantify partial F1 hybrid inviability and extensive F2 hybrid inviability; the ~75% F2 embryonic arrest occurs primarily during gastrulation or embryonic elongation. Moreover, F1 hybrid males exhibit Haldane's rule asymmetrically for both sterility and inviability, being strongest when C. remanei serves as maternal parent. We show that the mechanism by which sterile hybrid males are incapable of transferring sperm or a copulatory plug involves defective gonad morphogenesis, which we hypothesize results from linker cell defects in migration and/or cell death during development. This first documented case of partial hybrid male sterility in Caenorhabditis follows expectations of Darwin's corollary to Haldane's rule for asymmetric male fitness, providing a powerful foundation for molecular dissection of intrinsic reproductive barriers and divergence of genetic pathways controlling organ morphogenesis.

  8. pRB 、E2F1 在肺神经内分泌肿瘤 中的表达及其临床意义%The expression and clinical significance of pRB and E2F1 in human neuroendocrine lung tumor

    Institute of Scientific and Technical Information of China (English)

    田燕雏; 葛炳生; 赵凤瑞; 罗杰; 张晶; 赵恭华; 李廷

    2001-01-01

    目的 探索pRB和E2F1 mRNA在细胞周期中的作用及人肺神经内分泌肿瘤的发生机制;以及与肿瘤侵袭性和预后的相关性。方法 分别采用免疫组化和分子原位杂交技术对78例人肺神经内分泌肿瘤的石蜡切片标本中的pRB和E2F1mRNA表达进行检测,通过统计学方法研究其表达与肿瘤分期、淋巴结转移及预后的相互关系。结果 pRB在典型类癌和非典型类癌中的阳性表达率为87.5%(14/16),而在大细胞神经内分泌癌和小细胞肺癌中则不表达(P<0.001);E2F1 mRNA在人肺神经内分泌肿瘤中的阳性表达率为87.2%(68/78),二者的表达强度及相互关系与肿瘤的恶性程度、淋巴结转移及预后相关(P<0.05)。结论 pRB丢失是人肺神经内分泌肿瘤发生恶性变的关键。E2F1过度表达是人肺神经内分泌肿瘤发生的一个重要因素。%Objective To investigate the role pRB and E2F1 play in the cell cycle and the oncogenesis of human nemoendocrine lung fumor and the correlation between their expression and the invasiveness and prognosis of human neuroendocrine lung tumor. Methods Immunohistochemistry and in situ hybridization were used to detect the expression of pRB and E2F1 mRNA in the paraffin embedded tissues of 78 cases of human neuroendocrine lung tumor. Statistics was used to analyze the data. Results The positive expression rate of pRB in both typical and atypical carcinoid was 87.5%(14/16). However, pRB was not expressed in large cell neuroendocrine carcinoma and small cell lung cancer (P<0.001). The positive rate of E2F1 mRNA expression in human neuroendocrine lung tumor was 87.2% (68/78). The strength of expression of pRB and E2F1 was obviously correlated with the tumor stage, lymph node metastasis and prognosis (P<0.05). Conclusion The loss of pRB and E2F1 overexpression may play a key role in the development of human neuroendocrine lung tumors.

  9. 17 CFR 240.17f-1 - Requirements for reporting and inquiry with respect to missing, lost, counterfeit or stolen...

    Science.gov (United States)

    2010-04-01

    ... inquiry with respect to missing, lost, counterfeit or stolen securities. 240.17f-1 Section 240.17f-1... and inquiry with respect to missing, lost, counterfeit or stolen securities. (a) Definitions. For... not represented by an instrument and the transfer of which is registered upon books maintained...

  10. Expression of genes encoding F-1-ATPase results in uncoupling of glycolysis from biomass production in Lactococcus lactis

    DEFF Research Database (Denmark)

    Købmann, Brian Jensen; Solem, Christian; Pedersen, M.B.

    2002-01-01

    We studied how the introduction of an additional ATP-consuming reaction affects the metabolic fluxes in Lactococcus lactis. Genes encoding the hydrolytic part of the F-1 domain of the membrane-bound (F1F0) H+-ATPase were expressed from a range of synthetic constitutive promoters. Expression...

  11. Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ε.

    Science.gov (United States)

    Roy, Ankoor; Hutcheon, Marcus L; Duncan, Thomas M; Cingolani, Gino

    2012-10-01

    The bacterial ATP synthase (F(O)F(1)) of Escherichia coli has been the prominent model system for genetics, biochemical and more recently single-molecule studies on F-type ATP synthases. With 22 total polypeptide chains (total mass of ∼529 kDa), E. coli F(O)F(1) represents nature's smallest rotary motor, composed of a membrane-embedded proton transporter (F(O)) and a peripheral catalytic complex (F(1)). The ATPase activity of isolated F(1) is fully expressed by the α(3)β(3)γ 'core', whereas single δ and ε subunits are required for structural and functional coupling of E. coli F(1) to F(O). In contrast to mitochondrial F(1)-ATPases that have been determined to atomic resolution, the bacterial homologues have proven very difficult to crystallize. In this paper, we describe a biochemical strategy that led us to improve the crystallogenesis of the E. coli F(1)-ATPase catalytic core. Destabilizing the compact conformation of ε's C-terminal domain with a phosphomimetic mutation (εS65D) dramatically increased crystallization success and reproducibility, yielding crystals of E. coli F(1) that diffract to ∼3.15 Å resolution.

  12. The Role of a Novel Nucleolar Protein in Regulation of E2F1 in Breast Cancer

    Science.gov (United States)

    2009-09-01

    copresence of RRP1B and E2F1 on specific E2F1 targets (see below), We would now like to discuss progress in the research program. Task 1...A similar assay was performed identically except that cells were grown in media containing 2% fetal bovine serum. Real time and semiquantitative

  13. Positive and negative regulation of cell proliferation by E2F-1: influence of protein level and human papillomavirus oncoproteins

    DEFF Research Database (Denmark)

    Melillo, R M; Helin, K; Lowy, D R;

    1994-01-01

    E2F-1 is a member of a family of transcription factors implicated in the activation of genes required for the progression through the S phase of the cell cycle. We have examined the biological activities of E2F-1 with short-term colony-forming assays and long-term immortalization assays. High lev...

  14. The role of epigenetics in hybrid vigour.

    Science.gov (United States)

    Groszmann, Michael; Greaves, Ian K; Fujimoto, Ryo; Peacock, W James; Dennis, Elizabeth S

    2013-12-01

    Hybrid vigour, or heterosis, refers to the increased yield and biomass of hybrid offspring relative to the parents. Although this has been exploited in plants for agriculture and horticulture, the molecular and cellular mechanisms underlying hybrid vigour are largely unknown. Genetic analyses show that there are a large number of quantitative trait loci (QTLs) that contribute to the heterotic phenotype, indicating that it is a complex phenomenon. Gene expression in hybrids is regulated by the interactions of the two parental epigenetic systems and the underlying genomes. Increasing understanding of the interplay of small RNA (sRNA) molecules, DNA methylation, and histone marks provides new opportunities to define the basis of hybrid vigour and to understand why F1 heterosis is not passed on to subsequent generations. We discuss recent findings that suggest the existence of several pathways that alter DNA methylation patterns, which may lead to transcriptional changes resulting in the heterotic phenotype.

  15. y=f(x+1)与y=f-1(x+1)互为反函数吗?

    Institute of Scientific and Technical Information of China (English)

    邓先会; 鲁荣光

    2002-01-01

    @@ 从图象上看y=f(x+1)与y=f-1(x+1)的图象是分别将y=f(x)与y=f-1(x)的图象向左平移一个单位所得,因y=f(x)与y=f-1(x)图象关于y=x对称,将y=x向左平移一个单位得y=x+1,所以函数y=f(x+1)与y=f-1(x+1)的图象关于y=x+1对称,因而y=f(x+1)与y=f-1(x+1)不一定互为反函数.

  16. Lack of ability of trypsin-treated mitochondrial F1-ATPase to bind the oligomycin-sensitivity conferring protein (OSCP).

    Science.gov (United States)

    Hundal, T; Norling, B; Ernster, L

    1983-10-03

    Soluble beef-heart mitochondrial F1-ATPase modified in its alpha-subunit by mild trypsin treatment (alpha'-F1) can no longer bind oligomycin-sensitivity conferring protein (OSCP) but is still capable of binding to F1-depleted submitochondrial particles, giving rise to a maximally oligomycin-sensitive ATPase, provided the particles contain their native complement of OSCP. When OSCP is removed from the particles, alpha'-F1 can still bind to the particles, but added OSCP induces only a low degree of oligomycin sensitivity. The possible role of OSCP in the functional coupling of the catalytic (F1) and H+-translocating (Fo) moieties of mitochondrial ATPase is discussed. The results suggest a functional similarity between the OSCP component of mitochondrial ATPase and the delta-subunit of E. coli ATPase, which is in accordance with the structural homology recently found to exist between the two polypeptides.

  17. Dale Reed with model in front of M2-F1

    Science.gov (United States)

    1967-01-01

    Dale Reed with a model of the M2-F1 in front of the actual lifting body. Reed used the model to show the potential of the lifting bodies. He first flew it into tall grass to test stability and trim, then hand-launched it from buildings for longer flights. Finally, he towed the lifting-body model aloft using a powered model airplane known as the 'Mothership.' A timer released the model and it glided to a landing. Dale's wife Donna used a 9 mm. camera to film the flights of the model. Its stability as it glided--despite its lack of wings--convinced Milt Thompson and some Flight Research Center engineers including the center director, Paul Bikle, that a piloted lifting body was possible. The lifting body concept evolved in the mid-1950s as researchers considered alternatives to ballistic reentries of piloted space capsules. The designs for hypersonic, wingless vehicles were on the boards at NASA Ames and NASA Langley facilities, while the US Air Force was gearing up for its Dyna-Soar program, which defined the need for a spacecraft that would land like an airplane. Despite favorable research on lifting bodies, there was little support for a flight program. Dryden engineer R. Dale Reed was intrigued with the lifting body concept, and reasoned that some sort of flight demonstration was needed before wingless aircraft could be taken seriously. In February 1962, he built a model lifting body based upon the Ames M2 design, and air-launched it from a radio controlled 'mothership.' Home movies of these flights, plus the support of research pilot Milt Thompson, helped pursuade the facilities director, Paul Bikle, to give the go-ahead for the construction of a full-scale version, to be used as a wind-tunnel model and possibly flown as a glider. Comparing lifting bodies to space capsules, an unofficial motto of the project was, 'Don't be Rescued from Outer Space--Fly Back in Style.' The construction of the M2-F1 was a joint effort by Dryden and a local glider manufacturer, the

  18. EVALUATION OF THE REACTION OF WATERMELON PARENT AND F1 PLANTS TO Meloidogyne enterolobii

    Directory of Open Access Journals (Sweden)

    LÉIA SANTOS DAMACENO

    2016-01-01

    Full Text Available The aim of this study was to evaluate the performance of progenies from Citrullus lanatus var. lanatus (cultivated watermelons when crossed with progenies from C. lanatus var. citroides (fodder watermelon with a historic of resistance to the nematode Meloidogyne enterolobii. The parents and their F1s were evaluated for resistance to this nematode. In the initial stages of eleven treatments, watermelon seedlings plantlets were transplanted to plastic bags of six kilograms once the first leaves developed. Ten inoculated plants with 5,200 eggs in the soil near the stem of the plant and four non-inoculated ones were used in each treatment, in a complete block design. Sixty-two days after sowing, the following characteristics were evaluated: the length of the aerial part of the plant (LAP, in m, fresh mass of the aerial part (FMAP, in g, root fresh mass (RFM, in g, egg number (EN and reproduction factor (RF. A comparison between the averages of inoculated and non-inoculated plants was performed using Scott-Knott test at 5% and the diallelic analysis was performed using the GENES program. The morphological characteristics did not allow for the identification of the parent plants or the F1s with respect to nematode resistance, but the variables EN and RF were useful for such identification. The analyses of the general and specific combining abilities indicate highly significant effects with respect to this resistance, showing additive gene effects as well as dominance and epistatic gene effects, allowing for identification of parents and F1s that can be used in watermelon breeding programs to improve resistance to the M. enterolobii.

  19. Surface Plasmon Resonance Analysis of Histidine-Tagged F1-ATPase Surface Adsorption

    Science.gov (United States)

    Tucker, Jenifer K.; Richter, Mark L.; Berrie, Cindy L.

    2015-11-01

    Studies of the rotational activity of the enzymatic core (α3β3γ) of the F1-ATPase motor protein have relied on binding the enzyme to NTA-coated glass surfaces via polyhistidine tags engineered into the C-termini of each of the three α or β subunits. Those studies revealed the rotational motion of the central γ subunit by monitoring the motion of attached micron-long actin filaments or spherical nanoparticles. However, only a small percentage of the attached filaments or particles were observed to rotate, likely due, at least in part, to non-uniform surface attachment of the motor proteins. In this study, we have applied surface plasmon resonance to monitor the kinetics and affinity of binding of the His-tagged motor protein to NTA-coated gold sensor surfaces. The binding data, when fit to a heterogeneous binding model, exhibit two sets of adsorption-desorption rate constants with two dissociation constants of 4.0 × 10-9 M and 8.6 × 10-11 M for 6His-α3β3γ binding to the nickel ion-activated NTA surface. The data are consistent with mixed attachment of the protein via two (bimodal) and three (trimodal) NTA/Ni2+-His-tag interactions, respectively, with the less stable bimodal interaction dominating. The results provide a partial explanation for the low number of surface-attached F1 motors previously observed in rotation studies and suggest alternative approaches to uniform F1 motor surface attachment for future fabrication of motor-based nanobiodevices and materials.

  20. Red nucleus and rubrospinal tract disorganization in the absence of Pou4f1

    Science.gov (United States)

    Martinez-Lopez, Jesus E.; Moreno-Bravo, Juan A.; Madrigal, M. Pilar; Martinez, Salvador; Puelles, Eduardo

    2015-01-01

    The red nucleus (RN) is a neuronal population that plays an important role in forelimb motor control and locomotion. Histologically it is subdivided into two subpopulations, the parvocellular RN (pRN) located in the diencephalon and the magnocellular RN (mRN) in the mesencephalon. The RN integrates signals from motor cortex and cerebellum and projects to spinal cord interneurons and motor neurons through the rubrospinal tract (RST). Pou4f1 is a transcription factor highly expressed in this nucleus that has been related to its specification. Here we profoundly analyzed consequences of Pou4f1 loss-of-function in development, maturation and axonal projection of the RN. Surprisingly, RN neurons are specified and maintained in the mutant, no cell death was detected. Nevertheless, the nucleus appeared disorganized with a strong delay in radial migration and with a wider neuronal distribution; the neurons did not form a compacted population as they do in controls, Robo1 and Slit2 were miss-expressed. Cplx1 and Npas1, expressed in the RN, are transcription factors involved in neurotransmitter release, neuronal maturation and motor function processes among others. In our mutant mice, both transcription factors are lost, suggesting an abnormal maturation of the RN. The resulting altered nucleus occupied a wider territory. Finally, we examined RST development and found that the RN neurons were able to project to the spinal cord but their axons appeared defasciculated. These data suggest that Pou4f1 is necessary for the maturation of RN neurons but not for their specification and maintenance. PMID:25698939

  1. None of the Rotor Residues of F1-ATPase Are Essential for Torque Generation

    Science.gov (United States)

    Chiwata, Ryohei; Kohori, Ayako; Kawakami, Tomonari; Shiroguchi, Katsuyuki; Furuike, Shou; Adachi, Kengo; Sutoh, Kazuo; Yoshida, Masasuke; Kinosita, Kazuhiko

    2014-01-01

    F1-ATPase is a powerful rotary molecular motor that can rotate an object several hundred times as large as the motor itself against the viscous friction of water. Forced reverse rotation has been shown to lead to ATP synthesis, implying that the mechanical work against the motor’s high torque can be converted into the chemical energy of ATP. The minimal composition of the motor protein is α3β3γ subunits, where the central rotor subunit γ turns inside a stator cylinder made of alternately arranged α3β3 subunits using the energy derived from ATP hydrolysis. The rotor consists of an axle, a coiled coil of the amino- and carboxyl-terminal α-helices of γ, which deeply penetrates the stator cylinder, and a globular protrusion that juts out from the stator. Previous work has shown that, for a thermophilic F1, significant portions of the axle can be truncated and the motor still rotates a submicron sized bead duplex, indicating generation of up to half the wild-type (WT) torque. Here, we inquire if any specific interactions between the stator and the rest of the rotor are needed for the generation of a sizable torque. We truncated the protruding portion of the rotor and replaced part of the remaining axle residues such that every residue of the rotor has been deleted or replaced in this or previous truncation mutants. This protrusionless construct showed an unloaded rotary speed about a quarter of the WT, and generated one-third to one-half of the WT torque. No residue-specific interactions are needed for this much performance. F1 is so designed that the basic rotor-stator interactions for torque generation and control of catalysis rely solely upon the shape and size of the rotor at very low resolution. Additional tailored interactions augment the torque to allow ATP synthesis under physiological conditions. PMID:24853745

  2. Binding and hydrolysis of TNP-ATP by Escherichia coli F1-ATPase.

    Science.gov (United States)

    Weber, J; Senior, A E

    1996-02-16

    It had previously been suggested that Vmax hydrolysis rate of 2', 3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate (TNP-ATP) by F1-ATPase required filling of only two catalytic sites on the enzyme (Grubmeyer, C., and Penefsky, H. S. (1981) J. Biol. Chem. 256, 3718-3727), whereas recently it was shown that Vmax rate of ATP hydrolysis requires that all three catalytic sites are filled (Weber, J., Wilke-Mounts, S., Lee, R. S. F., Grell, E., and Senior, A. E. (1993) J. Biol. Chem. 268, 20126-20133). To resolve this apparent discrepancy, we measured equilibrium binding and hydrolysis of MgTNP-ATP under identical conditions, using betaY331W mutant Escherichia coli F1-ATPase, in which the genetically engineered tryptophan provides a direct fluorescent probe of catalytic site occupancy. We found that MgTNP-ATP hydrolysis at Vmax rate did require filling of all three catalytic sites, but in contrast to the situation with MgATP, "bisite hydrolysis" of MgTNP-ATP amounted to a substantial fraction (approximately 40%) of Vmax. Binding of MgTNP-ATP to the three catalytic sites showed strong binding cooperativity (Kd1 e. in presence of EDTA) bound to all three catalytic sites with lower affinity but was not hydrolyzed. These data emphasize that the presence of Mg2+ is critical for cooperativity of substrate binding, formation of the very high affinity first catalytic site, and hydrolytic activity in F1-ATPases and that these three properties are strongly correlated.

  3. Classifying genotype F of hepatitis B virus into F1 and F2 subtypes

    Institute of Scientific and Technical Information of China (English)

    Hideaki Kato; Takanobu Kato; Yuzo Miyakawa; Masashi Mizokami; Kei Fujiwara; Robert G. Gish; Hiroshi Sakugawa; Hiroshi Yoshizawa; Fuminaka Sugauchi; Etsuro Orito; Ryuzo Ueda; Yasuhito Tanaka

    2005-01-01

    AIM: To explore the propriety of providing hepatitis B virus(HBV) genotypes F and H with two distinct genotypes.METHODS: Eleven HBV isolates of genotype F (HBV/F)were recovered from patients living in San Francisco,Japan, Panama, and Venezuela, and their full-length sequences were determined. Phylogenetic analysis was carried out among them along with HBV isolates previously reported.RESULTS: Seven of them clustered with reported HBV/F isolates in the phylogenetic tree constructed on the entire genomic sequence. The remaining four flocked on another branch along with three HBV isolates formerly reported as genotype H. These seven HBV isolates, including the four in this study and the three reported, had a sequence divergence of 7.3-9.5% from the other HBV/F isolates,and differed by > 13.7% from HBV isolates of the other six genotypes (A-E and G). Based on a marked genomic divergence, falling just short of >8% separating the seven genotypes, these seven HBV/F isolates were classified into F2 subtype and the former seven into F1 subtype provisionally. In a pairwise comparison of the S-gene sequences among the 7 HBV/F2 isolates and against 47HBV/F1 isolates as well as 136 representing the other six genotypes (A-E and G), two clusters separated by distinct genetic distances emerged.CONCLUSION: Based on these analyses, classifying HBV/F isolates into two subtypes (F1 and F2) would be more appropriate than providing them with two distinct genotypes (F and H).

  4. New orbit recalculations of comet C/1890 F1 Brooks and its dynamical evolution

    Science.gov (United States)

    Królikowska, Małgorzata; Dybczyński, Piotr A.

    2016-08-01

    C/1890 F1 Brooks belongs to a group of 19 comets used by Jan Oort to support his famous hypothesis on the existence of a spherical cloud containing hundreds of billions of comets with orbits of semi-major axes between 50 000 and 150 000 au. Comet Brooks stands out from this group because of a long series of astrometric observations as well as a nearly 2-yr-long observational arc. Rich observational material makes this comet an ideal target for testing the rationality of an effort to recalculate astrometric positions on the basis of original (comet-star) measurements using modern star catalogues. This paper presents the results of such a new analysis based on two different methods: (i) automatic re-reduction based on cometary positions and the (comet-star) measurements and (ii) partially automatic re-reduction based on the contemporary data for the reference stars originally used. We show that both methods offer a significant reduction in the uncertainty of orbital elements. Based on the most preferred orbital solution, the dynamical evolution of comet Brooks during three consecutive perihelion passages is discussed. We conclude that C/1890 F1 is a dynamically old comet that passed the Sun at a distance below 5 au during its previous perihelion passage. Furthermore, its next perihelion passage will be a little closer than during the 1890-1892 apparition. C/1890 F1 is interesting also because it suffered extremely small planetary perturbations when it travelled through the planetary zone. Therefore, in the next passage through perihelion, it will once again be a comet from the Oort spike.

  5. RAPD linkage mapping in a longleaf pine x slash pine F1 family.

    Science.gov (United States)

    Kubisiak, T L; Nelson, C D; Nance, W L; Stine, M

    1995-06-01

    Random amplified polymorphic DNAs (RAPDs) were used to construct linkage maps of the parent of a longleaf pine (Pinus palustris Mill.) slash pine (Pinus elliottii Englm.) F1 family. A total of 247 segregating loci [233 (1∶1), 14 (3∶1)] and 87 polymorphic (between parents), but non-segregating, loci were identified. The 233 loci segregating 1∶1 (testcross configuration) were used to construct parent-specific linkage maps, 132 for the longleaf-pine parent and 101 for the slash-pine parent. The resulting linkage maps consisted of 122 marker loci in 18 groups (three or more loci) and three pairs (1367.5 cM) for longleaf pine, and 91 marker loci in 13 groups and six pairs for slash pine (952.9 cM). Genome size estimates based on two-point linkage data ranged from 2348 to 2392 cM for longleaf pine, and from 2292 to 2372 cM for slash pine. Linkage of 3∶1 loci to testcross loci in each of the parental maps was used to infer further linkages within maps, as well as potentially homologous counterparts between maps. Three of the longleaf-pine linkage groups appear to be potentially homologous counterparts to four different slash-pine linkage groups. The number of heterozygous loci (previously testcross in parents) per F1 individual, ranged from 96 to 130. With the 87 polymorphic, but non-segregating, loci that should also be heterozygous in the F1 progeny, a maximum of 183-217 heterozygous loci could be available for mapping early height growth (EHG) loci and for applying genomic selection in backcross populations.

  6. Long-term trends in the ionospheric E and F1 regions

    Directory of Open Access Journals (Sweden)

    J. Bremer

    2008-05-01

    Full Text Available Ground based ionosonde measurements are the most essential source of information about long-term variations in the ionospheric E and F1 regions. Data of such observations have been derived at many different ionospheric stations all over the world some for more than 50 years. The standard parameters foE, h'E, and foF1 are used for trend analyses in this paper. Two main problems have to be considered in these analyses. Firstly, the data series have to be homogeneous, i.e. the observations should not be disturbed by artificial steps due to technical reasons or changes in the evaluation algorithm. Secondly, the strong solar and geomagnetic influences upon the ionospheric data have carefully to be removed by an appropriate regression analysis. Otherwise the small trends in the different ionospheric parameters cannot be detected.

    The trends derived at individual stations differ markedly, however their dependence on geographic or geomagnetic latitude is only small. Nevertheless, the mean global trends estimated from the trends at the different stations show some general behaviour (positive trends in foE and foF1, negative trend in h'E which can at least qualitatively be explained by an increasing atmospheric greenhouse effect (increase of CO2 content and other greenhouse gases and decreasing ozone values. The positive foE trend is also in qualitative agreement with rocket mass spectrometer observations of ion densities in the E region. First indications could be found that the changing ozone trend at mid-latitudes (before about 1979, between 1979 until 1995, and after about 1995 modifies the estimated mean foE trend.

  7. Red nucleus and rubrospinal tract disorganization in the absence of Pou4f1

    Directory of Open Access Journals (Sweden)

    Jesus E. eMartinez-Lopez

    2015-02-01

    Full Text Available The red nucleus is a neuronal population that plays an important role in forelimb motor control and locomotion. Histologically it is subdivided into two subpopulations, the parvocellular red nucleus located in the diencephalon and the magnocellular red nucleus in the mesencephalon. The red nucleus integrates signals from motor cortex and cerebellum and projects to spinal cord interneurons and motor neurons through the rubrospinal tract. Pou4f1 is a transcription factor highly expressed in this nucleus that has been related to its specification. Here we profoundly analyzed consequences of Pou4f1 loss-of-function in development, maturation and axonal projection of the red nucleus. Surprisingly, red nucleus neurons are specified and maintained in the mutant, no cell death was detected. Nevertheless, the nucleus appeared disorganized with a strong delay in radial migration and with a wider neuronal distribution; the neurons did not form a compacted population as they do in controls, Robo1 and Slit2 were miss-expressed. Cplx1 and Npas1, expressed in the red nucleus, are transcription factors involved in neurotransmitter release, neuronal maturation and motor function processes among others. In our mutant mice, both transcription factors are lost, suggesting an abnormal maturation of the red nucleus. The resulting altered nucleus occupied a wider territory. Finally, we examined rubrospinal tract development and found that the red nucleus neurons were able to project to the spinal cord but their axons appeared defasciculated. These data suggest that Pou4f1 is necessary for the maturation of red nucleus neurons but not for their specification and maintenance.

  8. Studies of carcinogenicity of sodium chlorite in B6C3F1 mice.

    OpenAIRE

    Yokose, Y; Uchida, K; Nakae, D; Shiraiwa, K; Yamamoto, K; Konishi, Y.

    1987-01-01

    The carcinogenic activities of sodium chlorite in B6C3F1 mice were examined. Sodium chlorite was given at concentrations of 0 (control), 0.025% (low dose), or 0.05% (high dose) in the drinking water of 150 female and 150 male mice for 80 weeks, after which time the animals were returned to distilled water without sodium chlorite. All mice were sacrificed 85 weeks from the beginning of the experiment. The incidence of tumor-bearing animals was 32% (control), 34% (low dose), and 26% (high dose)...

  9. F1车队建立长期伙伴关系

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    最近,英国渥金。爱国者(aigo)和迈凯轮F1车队(Vodafone McLarenMercedes)宣布了长期伙伴关系,合作将从2007年1月1日开始。爱国者是与这支久负盛名的车队建立全球品牌合作的第一个中国品牌。

  10. Metabolic Trade-offs in Yeast are Caused by F1F0-ATP synthase

    DEFF Research Database (Denmark)

    Nilsson, Avlant; Nielsen, Jens

    2016-01-01

    of intermediary metabolism and consequently metabolic trade-offs may take place. One such trade-off, aerobic fermentation, occurs in both yeast (the Crabtree effect) and cancer cells (the Warburg effect) and has been a scientific challenge for decades. Here we show, using flux balance analysis combined...... of enzymes. The catalytic efficiency is also higher for cells grown on glucose compared to galactose and ethanol, which may explain the observed differences in their growth rates. The enzyme F1F0-ATP synthase (Complex V) was found to have flux control over respiration in the model, and since...

  11. Mineral requirements for growth and maintenance of F1 Boer × Saanen male kids.

    Science.gov (United States)

    Teixeira, I A M A; Härter, C J; Pereira Filho, J M; Sobrinho, A G da Silva; Resende, K T

    2015-05-01

    The objective of this study was to determine the net requirements of minerals for the growth and maintenance of intact male F1 Boer × Saanen goat kids in the initial phase of growth. The following 2 experiments were performed: Exp. 1 was performed to determine the net growth requirements for Ca, P, Mg, Na, and K by F1 Boer × Saanen goat kids from 5 to 25 kg of BW and Exp. 2 was performed to determine the maintenance requirements of F1 Boer × Saanen goats from 15 to 25 kg BW. In Exp. 1, 32 intact male goat kids were distributed in a completely randomized design and mineral body composition was fit to an allometric equation in the form of a nonlinear model. To determine the mineral requirements for maintenance in Exp. 2, 21 intact male goat kids were distributed in a randomized block design, where the goat kids were subjected to 3 levels of feed restriction (0, 30, and 60% feed restriction). At the onset of Exp. 2, 7 goat kids were harvested and used to estimate the initial body composition (15 kg BW). Initial body composition was used to calculate the retention of minerals. The maintenance requirements were estimated by regressions obtained from the retention of minerals in the empty body and the intake of the mineral. The concentration of Ca, P, Na, and K in the empty BW decreased by 11, 13, 26, and 23% with the increase in BW from 5 to 25 kg (P requirements of Ca, P, Mg, Na, and K for weight gain decreased by 27.5, 27.8, 4.25, 43.2, and 39.7%, respectively, with the increase in BW from 5 to 25 kg (P requirements (g/kg of ADG) decreased from 9.7 to 7.0 for Ca, 6.5 to 4.7 for P, 0.38 to 0.36 for Mg, 0.88 to 0.50 for Na, and 1.9 to 1.2 for K when BW increased from 5 to 25 kg. The daily net requirements for maintenance per kilogram of BW were 38 mg of Ca, 42 mg of P, 1.6 mg of Mg, 5.0 mg of Na, and 19 mg of K. These results for the nutritional requirements of minerals may help to formulate more balanced diets for F1 Boer × Saanen goat kids in the initial growth

  12. Clostridium pasteurianum F1Fo ATP Synthase: Operon, Composition, and Some Properties

    OpenAIRE

    2003-01-01

    The atp operon encoding F1Fo ATP synthase in the fermentative obligate anaerobic bacterium Clostridium pasteurianum was sequenced. It consisted of nine genes arranged in the order atpI(i), atpB(a), atpE(c), atpF(b), atpH(δ), atpA(α), atpG(γ), atpD(β), and atpC(ɛ), which was identical to that found in many bacteria. Reverse transcription-PCR confirmed the presence of the transcripts of all nine genes. The amount of ATPase activity in the membranes of C. pasteurianum was low compared to what ha...

  13. Enhanced inhibitory effects of TBT chloride on the development of F1 rats.

    Science.gov (United States)

    Asakawa, H; Tsunoda, M; Kaido, T; Hosokawa, M; Sugaya, C; Inoue, Y; Kudo, Y; Satoh, T; Katagiri, H; Akita, H; Saji, M; Wakasa, M; Negishi, T; Tashiro, T; Aizawa, Y

    2010-05-01

    Neurotoxicity is one of the major effects of tributyltin (TBT). The effects on the next generation of F(1) rats exposed to TBT via the placenta and their dams' milk may be stronger than those on adults. Pregnant Wister rats were exposed to TBT at 0 and 125 ppm in their food. Half of the female F(1) rats in both groups were exposed to TBT at 125 ppm in their food from 9 to 15 weeks of age. Female F(1) rats were divided into the following groups: the control-control (CC) group, with no exposure; the TBT-control (TC) group, exposed to TBT via the placenta and their dams' milk; the control-TBT (CT) group, exposed to TBT via their food from 9 to 15 weeks of age; and the TBT-TBT (TT) group, exposed to TBT via the placenta, their dams' milk, and their food (n = 10/group). After administration, an open-field test and prepulse inhibition (PPI) test were performed at 15 weeks of age. The mean body weights of the TC and TT groups were significantly lower than that of the CC group from 9 to 15 weeks of age. The mean relative thymus weight of the TC and TT groups was significantly lower than that of the CC group. In the open-field test, a marked decrease in the total locomotion distance was observed in the TT group. The mean values in the TT and TC groups were significantly lower than that in the CC group. For the locomotion distance between 15 and 20 min, the mean values in the CT, TC, and TT groups were significantly lower than that in the CC group. The mean locomotor distance between 25 and 30 min in the TT group was significantly lower than that in the CC and TC groups. The mean values of instances of wall rearing in the TC, CT, and TT groups were significantly lower than that in the CC group. The mean value of face washing or body washing in the TT group was significantly lower than that in the CT group. There were no significant differences in indexes of the PPI test. Exposure to TBT via the placenta and their dams' milk inhibited the development of F(1) rats, which

  14. 奔驰F1引擎公司换帅

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    12月10日,迈凯轮F1车队引擎供应商梅赛德斯一伊尔莫(Mercedes-llmor)公司宣布,他们将迎来新的常务董事奥拉-卡耶伦斯(Ola Kaellenius)。此人以前负责迈凯轮的道路跑车项目,大名鼎鼎的迈凯轮SLR便出自他之手。

  15. Metabolism of methapyrilene by Fischer-344 rat and B6C3F1 mouse hepatocytes.

    Science.gov (United States)

    Kelly, D W; Holder, C L; Korfmacher, W A; Getek, T A; Lay, J O; Casciano, D A; Shaddock, J G; Duhart, H M; Slikker, W

    1992-12-01

    1. Suspension cultures of freshly isolated F344 rat and B6C3F1 mouse hepatocytes were compared for their ability to transform various concentrations of methapyrilene (MP). 2. MP metabolites were isolated and purified by h.p.l.c., and were identified by comparing their chromatographic and mass spectral properties with those of authentic standards. 3. Both rat and mouse hepatocytes transformed MP to tentatively identified 2-thiophenecarboxylic acid (I), and definitively identified mono-N-desmethyl methapyrilene glucuronide (II), methapyrilene glucuronide (III), methapyrilene N-oxide (V), and mono-N-desmethyl methapyrilene (VII).

  16. Modeling of TCE and Toluene Toxicity to Pseudomonas putida F1

    Science.gov (United States)

    Singh, R.; Olson, M. S.

    2009-12-01

    Prediction of viable bacterial distribution with respect to contaminants is important for efficient bioremediation of contaminated ground-water aquifers, particularly those contaminated with residual NAPLs. While bacterial motility and chemotaxis may help situate bacteria close to high concentrations of contaminant thereby enhancing bioremediation, prolonged exposure to high concentrations of contaminates is toxic to contaminant-degrading bacteria. The purpose of this work is to model the toxicity of trichloroethylene and toluene to Pseudomonas putida F1. The Live/Dead® bacterial viability assay was used to determine the toxic effect of chemical contaminants on the viability of P. putida F1 in a sealed zero head-space experimental environment. Samples of bacterial suspensions were exposed to common ground-water pollutants, TCE and toluene, for different durations. Changes in live and dead cell populations were monitored over the course of experiments using fluorescence microscopy. Data obtained from these toxicity experiments were fit to simple linear and exponential bacterial decay models using non-linear regression to describe loss of bacterial viability. TCE toxicity to P. putida F1 was best described with an exponential decay model (Figure 1a), with a decay constant kTCE = 0.025 h-4.95 (r2 = 0.956). Toluene toxicity showed a marginally better fit to the linear decay model (Figure 1b) (r2 = 0.971), with a decay constant ktoluene = 0.204 h-1. Best-fit model parameters obtained for both TCE and toluene were used to predict bacterial viability in toxicity experiments with higher contaminant concentrations and matched well with experimental data. Results from this study can be used to predict bacterial accumulation and viability near NAPL sources, and thus may be helpful in improving bioremediation performance assessment of contaminated sites. Figure 1: Survival ratios (S = N/No) of P. putida F1 in TCE- (a) and toluene- (b) stressed samples (observed (

  17. Fluoroaluminum and fluoroberyllium nucleoside diphosphate complexes as probes of the enzymatic mechanism of the mitochondrial F1-ATPase.

    Science.gov (United States)

    Issartel, J P; Dupuis, A; Lunardi, J; Vignais, P V

    1991-05-14

    The mechanism by which fluoride and aluminum or beryllium in combination with ADP inhibit beef heart mitochondrial F1-ATPase was investigated. The kinetics of inhibition depended on the nature of the anion present in the F1-ATPase assay medium. Inhibition required the presence of Mg2+ and developed more rapidly with sulfite and sulfate than with chloride, i.e., with anions which activate F1-ATPase activity. The ADP-fluorometal complexes were bound quasi-irreversibly to F1, and each mole of the inhibitory nucleotide-fluorometal complex was tightly associated with 1 mol of Mg2+. One mole of nucleotide-fluorometal complex was able to inhibit the activity of 1 mol of catalytic site in F1. Direct measurements of bound fluoride, aluminum, beryllium, and ADP indicated that the F1-bound ADP-fluorometal complexes are of the following types: ADP1A11F4, ADP1Be1F1, ADP1Be1F2, or ADP1Be1F3. Fluoroaluminates or fluoroberyllates are isomorphous to Pi, and the inhibitory nucleotide-fluorometal complexes mimicked transient intermediates of nucleotides that appeared in the course of ATP hydrolysis. On the other hand, each mole of fully inhibited F1, retained 2 mol of inhibitory complexes. The same stoichiometry was observed when ADP was replaced by GDP, a nucleotide which, unlike ADP, binds only to the catalytic sites of F1. These results are discussed in terms of a stochastic model in which the three cooperative catalytic sites of F1 function in interactive pairs.

  18. Activation and inhibition of the Escherichia coli F1-ATPase by monoclonal antibodies which recognize the epsilon subunit.

    Science.gov (United States)

    Dunn, S D; Tozer, R G

    1987-02-15

    The properties of two monoclonal antibodies which recognize the epsilon subunit of Escherichia coli F1-ATPase were studied in detail. The epsilon subunit is a tightly bound but dissociable inhibitor of the ATPase activity of soluble F1-ATPase. Antibody epsilon-1 binds free epsilon with a dissociation constant of 2.4 nM but cannot bind epsilon when it is associated with F1-ATPase. Likewise epsilon cannot associate with F1-ATPase in the presence of high concentrations of epsilon-1. Thus epsilon-1 activates F1-ATPase which contains the epsilon subunit, and prevents added epsilon from inhibiting the enzyme. Epsilon-1 cannot bind to membrane-bound F1-ATPase. The epsilon-4 antibody binds free epsilon with a dissociation constant of 26 nM. Epsilon-4 can bind to the F1-ATPase complex, but, like epsilon-1, it reverses the inhibition of F1-ATPase by the epsilon subunit. The epsilon subunit remains crosslinkable to both the beta and gamma subunits in the presence of epsilon-4, indicating that it is not grossly displaced from its normal position by the antibody. Presumably the activation arises from more subtle conformational effects. Antibodies epsilon-4 and delta-2, which recognizes the delta subunit, both bind to F1F0 in E. coli membrane vesicles, indicating that these subunits are substantially exposed in the membrane-bound complex. Epsilon-4 inhibits the ATPase activity of the membrane-bound enzyme by about 50%, and Fab prepared from epsilon-4 inhibits by about 40%. This inhibition is not associated with any substantial change in the major apparent Km for ATP. These results suggest that inhibition of membrane-bound F1-ATPase arises from steric effects of the antibody.

  19. pRB-E2F1 complexes are resistant to adenovirus E1A-mediated disruption.

    Science.gov (United States)

    Seifried, L A; Talluri, S; Cecchini, M; Julian, L M; Mymryk, J S; Dick, F A

    2008-05-01

    Disruption of pRB-E2F interactions by E1A is a key event in the adenoviral life cycle that drives expression of early viral transcription and induces cell cycle progression. This function of E1A is complicated by E2F1, an E2F family member that controls multiple processes besides proliferation, including apoptosis and DNA repair. Recently, a second interaction site in pRB that only contacts E2F1 has been discovered, allowing pRB to control proliferation separately from other E2F1-dependent activities. Based on this new insight into pRB-E2F1 regulation, we investigated how E1A affects control of E2F1 by pRB. Our data reveal that pRB-E2F1 interactions are resistant to E1A-mediated disruption. Using mutant forms of pRB that selectively force E2F1 to bind through only one of the two binding sites on pRB, we determined that E1A is unable to disrupt E2F1's unique interaction with pRB. Furthermore, analysis of pRB-E2F complexes during adenoviral infection reveals the selective maintenance of pRB-E2F1 interactions despite the presence of E1A. Our experiments also demonstrate that E2F1 functions to maintain cell viability in response to E1A expression. This suggests that adenovirus E1A's seemingly complex mechanism of disrupting pRB-E2F interactions provides selectivity in promoting viral transcription and cell cycle advancement, while maintaining cell viability.

  20. Molecular cloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus%Molecular cloning and expression analysis of FTZ-F1 in the GIFT tilapia,Oreochromis niloticus

    Institute of Scientific and Technical Information of China (English)

    Jinling CAO; Jianjie CHEN; Zhongliang JIANG; Yongju LUO; Xi GAN

    2012-01-01

    The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia (gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.

  1. Replication of a chronic hepatitis B virus genotype F1b construct.

    Science.gov (United States)

    Hernández, Sergio; Jiménez, Gustavo; Alarcón, Valentina; Prieto, Cristian; Muñoz, Francisca; Riquelme, Constanza; Venegas, Mauricio; Brahm, Javier; Loyola, Alejandra; Villanueva, Rodrigo A

    2016-03-01

    Genotype F is one of the less-studied genotypes of human hepatitis B virus, although it is widely distributed in regions of Central and South American. Our previous studies have shown that HBV genotype F is prevalent in Chile, and phylogenetic analysis of its full-length sequence amplified from the sera of chronically infected patients identified it as HBV subgenotype F1b. We have previously reported the full-length sequence of a HBV molecular clone obtained from a patient chronically infected with genotype F1b. In this report, we established a system to study HBV replication based on hepatoma cell lines transfected with full-length monomers of the HBV genome. Culture supernatants were analyzed after transfection and found to contain both HBsAg and HBeAg viral antigens. Consistently, fractionated cell extracts revealed the presence of viral replication, with both cytoplasmic and nuclear DNA intermediates. Analysis of HBV-transfected cells by indirect immunofluorescence or immunoelectron microscopy revealed the expression of viral antigens and cytoplasmic viral particles, respectively. To test the functionality of the ongoing viral replication further at the level of chromatinized cccDNA, transfected cells were treated with a histone deacetylase inhibitor, and this resulted in increased viral replication. This correlated with changes posttranslational modifications of histones at viral promoters. Thus, the development of this viral replication system for HBV genotype F will facilitate studies on the regulation of viral replication and the identification of new antiviral drugs.

  2. Simple mechanism whereby the F1-ATPase motor rotates with near-perfect chemomechanical energy conversion.

    Science.gov (United States)

    Saita, Ei-ichiro; Suzuki, Toshiharu; Kinosita, Kazuhiko; Yoshida, Masasuke

    2015-08-04

    F1-ATPase is a motor enzyme in which a central shaft γ subunit rotates 120° per ATP in the cylinder made of α3β3 subunits. During rotation, the chemical energy of ATP hydrolysis (ΔGATP) is converted almost entirely into mechanical work by an elusive mechanism. We measured the force for rotation (torque) under various ΔGATP conditions as a function of rotation angles of the γ subunit with quasi-static, single-molecule manipulation and estimated mechanical work (torque × traveled angle) from the area of the function. The torque functions show three sawtooth-like repeats of a steep jump and linear descent in one catalytic turnover, indicating a simple physical model in which the motor is driven by three springs aligned along a 120° rotation angle. Although the second spring is unaffected by ΔGATP, activation of the first spring (timing of the torque jump) delays at low [ATP] (or high [ADP]) and activation of the third spring delays at high [Pi]. These shifts decrease the size and area of the sawtooth (magnitude of the work). Thus, F1-ATPase responds to the change of ΔGATP by shifting the torque jump timing and uses ΔGATP for the mechanical work with near-perfect efficiency.

  3. Maternal obesity and malnourishment exacerbate perinatal oxidative stress resulting in diabetogenic programming in F1 offspring.

    Science.gov (United States)

    Saad, M I; Abdelkhalek, T M; Haiba, M M; Saleh, M M; Hanafi, M Y; Tawfik, S H; Kamel, M A

    2016-06-01

    The effect of in-utero environment on fetal health and survival is long-lasting, and this is known as the fetal origin hypothesis. The oxidative stress state during gestation could play a pivotal role in fetal programming and development of diseases such as diabetes. In this study, we investigated the effect of intra-uterine obesity and malnutrition on oxidative stress markers in pancreatic and peripheral tissues of F1 offspring both prenatally and postnatally. Furthermore, the effect of postnatal diet on oxidative stress profile was evaluated. The results indicated that intra-uterine obesity and malnourishment significantly increased oxidative stress in F1 offspring. Moreover, the programming effect of obesity was more pronounced and protracted than malnutrition. The obesity-induced programming of offspring tissues was independent of high-caloric environment that the offspring endured; however, high-caloric diet potentiated its effect. In addition, pancreas and liver were the most affected tissues by fetal reprogramming both prenatally and postnatally. In conclusion, maternal obesity and malnutrition-induced oxidative stress could predispose offspring to insulin resistance and diabetes.

  4. Molecular marker heterozygosities and genetic distances as correlates of production traits in F1 bovine crosses

    Directory of Open Access Journals (Sweden)