Safety design of next generation SUI of CANDU stations

International Nuclear Information System (INIS)

Nasimi, Elnara; Gabbar, Hossam A.

2013-01-01

Highlights: ► Review of current SUI technologies and challenges. ► Propose a new type of SUI detectors. ► Propose a new SUI system architecture and layout. ► Propose implementation procedure for SUI with reduced risks. - Abstract: Due to the age and operating experience of Nuclear Power Plants, equipment ageing and obsolescence has become one of the main challenges that need to be resolved for all systems, structures and components in order to ensure a safe and reliable production of energy. This paper summarizes the research into a methodology for modernization of Start-Up Instrumentation (SUI), both in-core and Control Room equipment, using a new generation of detectors and cables in order to manage obsolescence. The main objective of this research is to develop a new systematic approach to SUI installation/replacement procedure development and optimization. Although some additional features, such as real-time data monitoring and storage/archiving solutions for SUI systems are also examined to take full advantage of today's digital technology, the objectives of this study do not include detailed parametrical studies of detector or system performance. Instead, a number of technological, operational and maintenance issues associated with Start-Up Instrumentation systems at Nuclear Power Plants (NPPs) will be identified and a structured approach for developing a replacement/installation procedure that can be standardized and used across all of the domestic CANDU (Canadian Deuterium Uranium) stations is proposed.

Scienze cognitive e game design. Progettare dinamiche di gioco non finalizzate a un obiettivo

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Pietro Righi Riva

2012-05-01

Full Text Available Stiamo vivendo gli anni della gamification: game designers e teorici del game design dibattono sui modi di trasporre meccaniche di gioco in servizi, applicazioni e marketing tools non strettamente legati al gioco. La gamification è finalizzata a determinare nel giocatore/utente un comportamento. Nella retorica che supporta la gamification traspare una visione del gioco fortemente orientata al reward, cioè alla gratificazione in termini di premi e obiettivi.

[Streptococcus suis meningitis in a meat factory employee].

Science.gov (United States)

de Ceuster, Laura M E; van Dillen, Jeroen J; Wever, Peter C; Rozemeijer, Wouter; Louwerse, Elisabeth S

2012-01-01

In the Netherlands, Streptococcus suis is a rare cause of meningitis. Over the past few years, the number of reported cases worldwide has increased. The bacterium is mainly isolated in pigs, but humans can also become infected. At the Emergency Department, a 60-year-old man presented with headache, confusion, fever and nuchal rigidity. He worked at a meat factory. Laboratory testing showed abnormalities linked to bacterial meningitis. S. suis was cultured from blood and cerebrospinal fluid. The patient was treated with dexamethasone, ceftriaxone and later benzylpenicillin intravenously. He recovered well, but had bilateral perceptive hearing loss as a sequela. Particularly people who are in close contact with pigs have an increased risk of S. suis infection. S. suis meningitis can be very severe and lead to serious complications and even death. Rapid diagnosis and adequate treatment are critical. Permanent hearing loss is the most frequent sequela.

Whole genome investigation of a divergent clade of the pathogen Streptococcus suis

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Abiyad eBaig

2015-11-01

Full Text Available Streptococcus suis is a major porcine and zoonotic pathogen responsible for significant economic losses in the pig industry and an increasing number of human cases. Multiple isolates of S. suis show marked genomic diversity. Here we report the analysis of whole genome sequences of nine pig isolates that caused disease typical of S. suis and had phenotypic characteristics of S. suis, but their genomes were divergent from those of many other S. suis isolates. Comparison of protein sequences predicted from divergent genomes with those from normal S. suis reduced the size of core genome from 793 to only 397 genes. Divergence was clear if phylogenetic analysis was performed on reduced core genes and MLST alleles. Phylogenies based on certain other genes (16S rRNA, sodA, recN and cpn60 did not show divergence for all isolates, suggesting recombination between some divergent isolates with normal S. suis for these genes. Indeed, there is evidence of recent recombination between the divergent and normal S. suis genomes for 249 of 397 core genes. In addition, phylogenetic analysis based on the 16S rRNA gene and 132 genes that were conserved between the divergent isolates and representatives of the broader Streptococcus genus showed that divergent isolates were more closely related to S. suis. Six out of nine divergent isolates possessed a S. suis-like capsule region with variation in capsular gene sequences but the remaining three did not have a discrete capsule locus. The majority (40/70, of virulence-associated genes in normal S. suis were present in the divergent genomes. Overall, the divergent isolates extend the current diversity of S. suis species but the phenotypic similarities and the large amount of gene exchange with normal S. suis gives insufficient evidence to assign these isolates to a new species or subspecies. Further sampling and whole genome analysis of more isolates is warranted to understand the diversity of the species.

Morphologic, biometric, and isoenzyme characterization of Trichuris suis.

Science.gov (United States)

Oliveros, R; Cutillas, C; Arias, P; Guevara, D

1998-06-01

Trichuris suis isolates were collected from the cecum of Sus scrofa domestica (pig) and S. s. scrofa (wild boar). Morphology and biometry studies were carried out. Morphology studies showed the existence of typical caudal papillae in males of T. suis from wild boars, but no other difference was observed in the biometric parameters (total length, esophageal length, posterior-portion body length, and spicular length) of T. suis isolated from either host. Individual extracts were subjected to malate dehydrogenase (MDH), malic enzyme (ME), glucose 6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH), and superoxide dismutase (SOD) isoenzyme analysis following starch-gel electrophoresis, and the isoenzyme patterns were compared with those obtained from other species of trichurids. MDH, ME, G6PD, LDH, and SOD isoenzyme patterns were identical for T. suis from both hosts. MDH isoenzyme patterns were characterized by the presence of one cathodic isoenzyme. ME, G6PD, and LDH isoenzyme patterns indicated the presence of three phenotypes, whereas the SOD isoenzyme pattern showed only one phenotype characterized by the existence of two (anodic and cathodic) bands. Different LDH and SOD isoenzyme patterns observed for T. suis, T. ovis, and T. skrjabini confirm once more that isoenzyme patterns have potential as a diagnostic tool for differentiation of different species of Trichuris.

Molecular diagnostics of swine infection caused by Mycoplasma suis

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Potkonjak Aleksandar

2009-01-01

Full Text Available The presence of two types of haemoplasm can be established in the swine population. Pathogenic haemoplasm, named Mycoplasma suis (previously called Eperythrozoon suis is the cause of swine eperythrozoonosis or swine ichtheroanaemia. The cause of this disease can also infect humans. The disease has spread all over the world. The most frequent form is latent infection of swine caused by M. suis. The disease is clinically manifest following action by the stress factor. The acute course of the disease is characterized by the occurrence of a febrile condition and ichtheroanaemia. The disease is usually diagnosed based on an epizootiological poll, a clinical examination, and a microscopic examination of a blood smear stained most often according to Giemsa. Contemporary methods of molecular biology have been developed, such as PCR, which are more sensitive and specific in making a diagnosis of swine infection caused by M. suis. In these investigations, the presence of M. suis on pig farms in the Republic of Serbia has been determined using the PCR test. .

[Serological detection of Brucella suis, influenza virus and Aujeszky's disease virus in backyard and small swine holders in Argentina].

Science.gov (United States)

Dibarbora, Marina; Cappuccio, Javier A; Aznar, María N; Bessone, Fernando A; Piscitelli, Hernán; Pereda, Ariel J; Pérez, Daniel R

Farmers raising less than 100 sows represent more than 99% of swine producers in Argentina, although little is known about their sanitary status and productive characteristics in the country. Sanitary and productive information was obtained. Furthermore, samples for serological studies were taken to detect antibodies against Brucella suis (Bs), Aujeszky's disease virus (AV) and influenza virus (IV) in 68 backyard and small producers with less than 100 sows located in the north, central and south regions of Argentina. Antibodies against H1 pandemic were detected in 80% of the farms while 11%, 11.7% and 6.0% of the producers were positive to influenza H3 cluster 2, AV and Bs, respectively. None of the producers was aware of the risk factors concerning the transmission of diseases from pigs to humans. A percentage of 47% of them buy pigs for breeding from other farmers and markets. With regard to biosecurity measures, only 16% of the farms had perimeter fences. The results of this study demonstrate that productive characterization and disease surveys are important to improve productivity and to reduce the risk of disease transmission among animals and humans. The study of sanitary status and risk factors is necessary for better control and eradication of diseases in backyard or small producers. More representative studies at country level should be carried out to detect the pathogensthat circulate and, with this knowledge, to implement prevention and control measures. Copyright © 2017 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

Sanitary-epidemiologic service in Perm region: 95th anniversary (dedicated to Russian Sanitary-epidemiologic service

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V.G. Kostarev

2017-09-01

Full Text Available The paper outlines the first stages of sanitary activities development in Perm region. We highlight the fact that it was Perm where I.I. Molessson, the first official sanitary inspector in Russia, worked. Here one of the first sanitary inspection of a whole region was performed and "Perm sanitary bulletin" was first published. It was in Perm, where the first sanitary office was established in 1890, and in 1898 the first bacteriological laboratory was organized. Bodies and institutions of Rospotrebnadzor in Perm region are known to be founded on September 15, 1922, when a Decree by Russian People's Commissars Council "On the Republic's sanitary bodies" was issued. A sanitary-hygienic faculty of Perm Medical Institute was set up in 1931 and it gave grounds for preparing qualified sanitary inspectors for state sanitary authorities. We described basic tasks which were tackled by sanitary inspectors during the Great Patriotic War and just after it. Rospotrebnadzor activities are proved to make for substantial improvements of sanitary-epidemiologic situation in the region. Currently more than 95 % of population are provided with drinking water which fully corresponds to the sanitary legislation requirements. We note constant improvements in catering objects quality and we also see that a quantity of food samples taken at such objects which don't correspond to hygienic standards remains stably low. A share of working places deviating from hygienic standards as per noise, vibration, and illumination, has reduced. Medical examinations are now available to 97.9 % of workers functioning under hazardous (dangerous working conditions. The lowest occupa-tional morbidity level over the last few years was registered in Perm region in 2016. The Service was among the first in the country to transfer to result-oriented budgeting and risk-oriented surveillance model. Organizational structure is constantly being developed; new legal, economic and organizational

Immune receptors involved in Streptococcus suis recognition by dendritic cells.

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Marie-Pier Lecours

Full Text Available Streptococcus suis is an important swine pathogen and an emerging zoonotic agent of septicemia and meningitis. Knowledge on host immune responses towards S. suis, and strategies used by this pathogen for subversion of these responses is scarce. The objective of this study was to identify the immune receptors involved in S. suis recognition by dendritic cells (DCs. Production of cytokines and expression of co-stimulatory molecules by DCs were shown to strongly rely on MyD88-dependent signaling pathways, suggesting that DCs recognize S. suis and become activated mostly through Toll-like receptor (TLR signaling. Supporting this fact, TLR2(-/- DCs were severely impaired in the release of several cytokines and the surface expression of CD86 and MHC-II. The release of IL-12p70 and CXC10, and the expression of CD40 were found to depend on signaling by both TLR2 and TLR9. The release of IL-23 and CXCL1 were partially dependent on NOD2. Finally, despite the fact that MyD88 signaling was crucial for DC activation and maturation, MyD88-dependent pathways were not implicated in S. suis internalization by DCs. This first study on receptors involved in DC activation by S. suis suggests a major involvement of MyD88 signaling pathways, mainly (but not exclusively through TLR2. A multimodal recognition involving a combination of different receptors seems essential for DC effective response to S. suis.

Population dynamics of Trichuris suis in trickle-infected pigs.

Science.gov (United States)

Nejsum, P; Thamsborg, S M; Petersen, H H; Kringel, H; Fredholm, M; Roepstorff, A

2009-05-01

The population dynamics of Trichuris suis in pigs was studied during long-term experimental infections. Twenty-three 10-week-old pigs were inoculated with 5 T. suis eggs/kg/day. Seven, 8, and 8 pigs were necropsied at weeks 4, 8, and 14 post-start of infection (p.i.), respectively. The median numbers of worms in the colon were 538 (min-max: 277-618), 332 (14-1140) and 0 (0-4) at 4, 8, and 14 weeks p.i. respectively, suggesting an increased aggregation of the worms with time and acquisition of nearly sterile immunity. The serum levels of T. suis specific antibodies (IgG1, IgG2 and IgA) peaked at week 8 p.i. By week 14 p.i. the IgG2 and IgA antibody levels remained significantly elevated above the level of week 0. The population dynamics of T. suis trickle infections in pigs is discussed with focus on interpretation of diagnostic and epidemiological data of pigs, the use of pigs as a model for human Trichuris trichiura infections and the novel approach of using T. suis eggs in the treatment of patients with inflammatory bowel disease.

The Complete Genome of Brucella Suis 019 Provides Insights on Cross-Species Infection

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Yuanzhi Wang

2016-01-01

Full Text Available Brucella species are the most important zoonotic pathogens worldwide and cause considerable harm to humans and animals. In this study, we presented the complete genome of B. suis 019 isolated from sheep (ovine with epididymitis. B. suis 019 has a rough phenotype and can infect sheep, rhesus monkeys and possibly humans. The comparative genome analysis demonstrated that B. suis 019 is closest to the vaccine strain B. suis bv. 1 str. S2. Further analysis associated the rsh gene to the pathogenicity of B. suis 019, and the WbkA gene to the rough phenotype of B. suis 019. The 019 complete genome data was deposited in the GenBank database with ID PRJNA308608.

Streptococcus suis Meningitis: A Systematic Review and Meta-analysis

Science.gov (United States)

van Samkar, Anusha; Brouwer, Matthijs C.; Schultsz, Constance; van der Ende, Arie; van de Beek, Diederik

2015-01-01

Background Streptococcus suis is the most common cause of meningitis in pork consuming and pig rearing countries in South-East Asia. We performed a systematic review of studies on S. suis meningitis to define the clinical characteristics, predisposing factors and outcome. Methodology Studies published between January 1, 1980 and August 1, 2015 were identified from main literature databases and reference lists. Studies were included if they were written in West-European languages and described at least 5 adult patients with S. suis meningitis in whom at least one clinical characteristic was described. Findings We identified 913 patients with S. suis meningitis included in 24 studies between 1980 and 2015. The mean age was 49 years and 581 of 711 patients were male (82%). Exposure to pigs or pork was present in 395 of 648 patients (61%) while other predisposing factors were less common. 514 of 528 patients presented with fever (97%), 429 of 451 with headache (95%), 462 of 496 with neck stiffness (93%) and 78 of 384 patients (20%) had a skin injury in the presence of pig/pork contact. The case fatality rate was 2.9% and hearing loss was a common sequel occurring in 259 of 489 patients (53%). Treatment included dexamethasone in 157 of 300 (52%) of patients and was associated with reduced hearing loss in S. suis meningitis patients included in a randomized controlled trial. Conclusion S. suis meningitis has a clear association with pig and pork contact. Mortality is low, but hearing loss occurs frequently. Dexamethasone was shown to reduce hearing loss. PMID:26505485

SUI CODICI UNIDIREZIONALI

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Luca Tallini

1992-04-01

Full Text Available Oltre ad una breve descrizione della problematica sui codici Unidirezionali è qui data la dimostrazione di una congettura di S. Al-Bassan e B. Bose che interviene nella teoria dei suddetti autori, sulla costruzione di codici ottimali nella classe dei codici bilanciati che si ottengono con il metodo della complementazione di Knuth.

More efficient services with the new EMAS; Piu' possibilita' per i servizi con il nuovo EMAS

Energy Technology Data Exchange (ETDEWEB)

Luciani, R; Andriola, L [ENEA, Casaccia, RM (Italy). Dipt. Ambiente

1999-12-01

The article discusses the new EMAS regulations, which are also applicable in the service sector. It has been described the pilot projects launched by ENEA (National Agency for New Technology, Energy and the Environment) in collaboration with the Ministry of Industry, Federturismo, Ministry of Environmental and the municipality of Modena, in the hotel sector and in public administration. [Italian] L'articolo discute il nuovo regolamanto EMAS che estende l'applicazione della certificazione ambientale anche all'area dei servizi. Si descrivono i progetti che l'ENEA ha condotto in collaborazione con il Ministero dell'Industria, il Ministero dell'Ambiente, Federturismo e il comune di Modena nel settore alberghiero e nella pubblica amministrazione.

Clearance of Streptococcus suis in Stomach Contents of Differently Fed Growing Pigs

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Franziska Warneboldt

2016-08-01

Full Text Available Streptococcus (S. suis translocates across the intestinal barrier of piglets after intraintestinal application. Based on these findings, an oro-gastrointestinal infection route has been proposed. Thus, the objective of this study was to investigate the survival of S. suis in the porcine stomach. Whereas surviving bacteria of S. suis serotypes 2 and 9 were not detectable after 60 min of incubation in stomach contents with a comparatively high gastric pH of 5 due to feeding of fine pellets, the number of Salmonella Derby bacteria increased under these conditions. Further experiments confirmed the clearance of S. suis serotypes 2 and 9 within 30 min in stomach contents with a pH of 4.7 independently of the bacterial growth phase. Finally, an oral infection experiment was conducted, feeding each of 18 piglets a diet mixed with 1010 CFU of S. suis serotype 2 or 9. Thorough bacteriological screenings of various mesenteric-intestinal lymph nodes and internal organs after different times of exposure did not lead to any detection of the orally applied challenge strains. In conclusion, the porcine stomach constitutes a very efficient barrier against oro-gastrointenstinal S. suis infections. Conditions leading to the passage of S. suis through the stomach remain to be identified.

Host-pathogen Interaction at the Intestinal Mucosa Correlates With Zoonotic Potential of Streptococcus suis

DEFF Research Database (Denmark)

Ferrando, Maria Laura; de Greeff, Astrid; van Rooijen, Willemien J. M.

2015-01-01

Background. Streptococcus suis has emerged as an important cause of bacterial meningitis in adults. The ingestion of undercooked pork is a risk factor for human S. suis serotype 2 (SS2) infection. Here we provide experimental evidence indicating that the gastrointestinal tract is an entry site of...... be considered a food-borne pathogen. S. suis interaction with human and pig IEC correlates with S. suis serotype and genotype, which can explain the zoonotic potential of SS2....... of SS2 infection. Methods. We developed a noninvasive in vivo model to study oral SS2 infection in piglets. We compared in vitro interaction of S. suis with human and porcine intestinal epithelial cells (IEC). Results. Two out of 15 piglets showed clinical symptoms compatible with S. suis infection 24......Background. Streptococcus suis has emerged as an important cause of bacterial meningitis in adults. The ingestion of undercooked pork is a risk factor for human S. suis serotype 2 (SS2) infection. Here we provide experimental evidence indicating that the gastrointestinal tract is an entry site...

Detection and transmission of extracellular fac-tor producing Streptococcus suis serotype 2 strains in pigs

NARCIS (Netherlands)

Swildens, B.

2009-01-01

DETECTION AND TRANSMISSION OF EXTRACELLULAR FACTOR PRODUCING STREPTOCOCCUS SUIS SEROTYPE 2 STRAINS IN PIGS INTRODUCTION Streptococcus suis (S.suis) has been implicated in the etiology of many diseases among which meningitis in pigs. The virulent extracellular factor-positive strains of S.suis

Swine infection by Streptococcus suis: a retrospective study Infecção em suínos por Streptococcus suis: estudo retrospectivo

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A.E. Del'Arco

2008-08-01

Full Text Available The epidemic aspects of swine infections caused by Streptococcus suis were studied, focusing mainly on the occurrence of several serotypes. A total of 323 samples of S. suis were isolated from clinically ill animals, serotyped according to the co-agglutination procedure, and analyzed. The serotyping revealed that S. suis was present in several Brazilian states. The largest number was isolated from the states of Minas Gerais (62.5%, São Paulo (10.8%, and Paraná (9.3%. Serotype 2 was the most frequent (61.0%, followed by the serotypes 1, 3, 4, 7, and 8. The largest number of isolations was obtained from the brain (60.1%, followed by the lungs (10.4%. About 9.4% of the cases were due to septicemia.Estudaram-se os aspectos epidêmicos das infecções de suínos causadas por Streptococcus suis, enfocando, principalmente, a ocorrência de diferentes sorotipos. Foram analisadas 323 amostras isoladas de animais clinicamente doentes, as quais foram sorotipadas de acordo com o procedimento de co-aglutinação. Foi verificado que S. suis está presente em vários estados brasileiros e o maior número de isolados originou-se dos estados de Minas Gerais (62,5%, São Paulo (10,8% e Paraná (9,3%. O sorotipo 2 foi o mais freqüente (61.0%, seguido pelos sorotipos 1, 3, 4, 7 e 8. Os isolamentos foram obtidos principalmente de cérebro (60,1% e pulmões (10,4%. Os casos de septicemia representaram 9,4%.

Piano dei Servizi. Proposal for Contents and Guideline

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Roberto Gerundo

2014-05-01

Full Text Available As an endless melting pot of experimentation and innovation, cities must reorganize and retrain compared to the growing new needs. In the light of the actual urban debate, it can be useful to do an in-depth study. In fact, the quality of urban life and collective well-being cannot be separated from the identification of a network of public services and facilities, that organizes and structures the city. That network is not resolved in the themes of pre-school and compulsory education, public interest, green spaces, car parks and public interest, but rather has a wider variety of types. Noting the failure of attempts to define, a priori, a quantity of universally valid services, it is necessary to rethink the ways and criteria of most of the infrastructural facilities. The "Piano dei Servizi" is the tool for the implementation of a concrete policy of services for public interest, and it means the transition between a planning standard, in terms of quantity, in a standard that meets quality requirements. It represents a specific section of the urban development plan, which has to consider problems and shortcomings, and try to solve them by finding opportunities which physical locations offer. In this way a planning standard is not configured as an abstract quantity, but as the material composition of the urban plan, which results in a land use and land development proposal.

Streptococcus suis meningitis can require a prolonged treatment course

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Jean Dejace

2017-12-01

Full Text Available We report a case of recrudescent Streptococcus suis meningitis requiring a prolonged treatment course. A few similar cases can be found in the burgeoning literature on what remains a relatively uncommon disease in humans, and these patients should be monitored carefully upon completion of therapy. Keywords: Meningitis, Relapse, Duration, Streptococcus suis

DEP Reported Sanitary Sewer Overflows

Data.gov (United States)

Montgomery County of Maryland — Sanitary sewer overflows reported to the Department of Environmental Protection by the Washington Suburban Sanitary Commission or individuals in the County. Update...

Sanitary effects of fossil fuels

International Nuclear Information System (INIS)

Nifenecker, H.

2006-01-01

In this compilation are studied the sanitary effects of fossil fuels, behavioral and environmental sanitary risks. The risks in connection with the production, the transport and the distribution(casting) are also approached for the oil(petroleum), the gas and the coal. Accidents in the home are evoked. The risks due to the atmospheric pollution are seen through the components of the atmospheric pollution as well as the sanitary effects of this pollution. (N.C.)

Inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from Mycoplasma suis

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Wittenbrink Max M

2010-07-01

Full Text Available Abstract Background Mycoplasma suis belongs to a group of highly specialized hemotrophic bacteria that attach to the surface of host erythrocytes. Hemotrophic mycoplasmas are uncultivable and the genomes are not sequenced so far. Therefore, there is a need for the clarification of essential metabolic pathways which could be crucial barriers for the establishment of an in vitro cultivation system for these veterinary significant bacteria. Inorganic pyrophosphatases (PPase are important enzymes that catalyze the hydrolysis of inorganic pyrophosphate PPi to inorganic phosphate Pi. PPases are essential and ubiquitous metal-dependent enzymes providing a thermodynamic pull for many biosynthetic reactions. Here, we describe the identification, recombinant production and characterization of the soluble (sPPase of Mycoplasma suis. Results Screening of genomic M. suis libraries was used to identify a gene encoding the M. suis inorganic pyrophosphatase (sPPase. The M. suis sPPase consists of 164 amino acids with a molecular mass of 20 kDa. The highest identity of 63.7% was found to the M. penetrans sPPase. The typical 13 active site residues as well as the cation binding signature could be also identified in the M. suis sPPase. The activity of the M. suis enzyme was strongly dependent on Mg2+ and significantly lower in the presence of Mn2+ and Zn2+. Addition of Ca2+ and EDTA inhibited the M. suis sPPase activity. These characteristics confirmed the affiliation of the M. suis PPase to family I soluble PPases. The highest activity was determined at pH 9.0. In M. suis the sPPase builds tetramers of 80 kDa which were detected by convalescent sera from experimentally M. suis infected pigs. Conclusion The identification and characterization of the sPPase of M. suis is an additional step towards the clarification of the metabolism of hemotrophic mycoplasmas and, thus, important for the establishment of an in vitro cultivation system. As an antigenic and conserved

Streptococcus suis, an emerging drug-resistant animal and human pathogen

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Claudio ePalmieri

2011-11-01

Full Text Available Streptococcus suis, a major porcine pathogen, has been receiving growing attention not only for its role in severe and increasingly reported infections in humans, but also for its involvement in drug resistance. Recent studies and the analysis of sequenced genomes have been providing important insights into the S. suis resistome, and have resulted in the identification of resistance determinants for tetracyclines, macrolides, aminoglycosides, chloramphenicol, antifolate drugs, streptothricin, and cadmium salts. Resistance gene-carrying genetic elements described so far include integrative and conjugative elements, transposons, genomic islands, phages, and chimeric elements. Some of these elements are similar to those reported in major streptococcal pathogens such as Streptococcus pyogenes, Streptococcus pneumoniae, and Streptococcus agalactiae and share the same chromosomal insertion sites. The available information strongly suggests that S. suis is an important antibiotic resistance reservoir that can contribute to the spread of resistance genes to the above-mentioned streptococci. S. suis is thus a paradigmatic example of possible intersections between animal and human resistomes.

Uptake of benzimidazoles by Trichuris suis in vivo in pigs

DEFF Research Database (Denmark)

Hansen, Tina Vicky Alstrup; Friis, Christian; Nejsum, Peter

2014-01-01

It is recognized that the clinical efficacy of single dose benzimidazoles (BZs) against the nematode, Trichuris suis of pigs and the closely related Trichuris trichiura in humans is only poor to moderate. Recent in vitro studies have indicated that a low uptake of fenbendazole (FBZ) in T. suis may...

Organization of setting-up sanitary pass-control regime and sanitary treatment of injured persons in case of radiation accidents

International Nuclear Information System (INIS)

Korostin, A.S.; Klochkov, V.N.; Yatsenko, V.N.

1995-01-01

The main aim of sanitary pass-control regime is to prevent propagation of radioactive contamination outside the area of emergency-rescue works and guarantee of sanitary treatment of all persons having radioactive contamination. The paper has studied the questions of organization of sanitary pass-control regime, arrangement of sanitary treatment of the injured persons and rendering first aid in case of radioactive contamination of wounds. 5 refs

Lexicon and Description of Sui Adjective Intensifiers

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James N. Stanford

2007-01-01

Full Text Available Sui, an indigenous minority language of southwest China, has an elaborate system of adjective intensification. Adjectives are intensified with word-specific, bound morphemes that usually either rhyme with the base or alliterate with the base. Stanford (2007 notes morpho-phonological patterns that suggest reduplication, rhyme, alliteration, The Emergence of the Unmarked (McCarthy & Prince 1994, Yip 2001, identity avoidance, and “Copy But Don’t Repeat” (Kennard 2004. However, the adjective intensifiers defy a simple, fully predictable explanation in such terms; the intensifier lexicon may be best described as “patterned variety,” a case of lexicalized poetry or a poeticized lexicon. Word formation is guided by general patterns, but each specific intensifier may vary within those overall guidelines. Many adjectives have multiple intensifiers that bear subtle semantic and pragmatic distinctions. The current paper serves as a complement to Stanford (2007 by providing a detailed lexicon of the Sui adjective intensifiers for future reference and further analysis. This lexicon is based on the author’s fieldwork and represents the first detailed account of Sui adjective intensifiers for the wider linguistic community.

Effect of spatial separation of pigs on spread of Streptococcus suis serotype 9.

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Niels Dekker

Full Text Available The spread of an infectious agent in a population can be reduced by interfering in the infectiousness or susceptibility of individuals, and/or in their contact structure. The aim of this study was to quantify the effect of prevention of direct contact between infectious and susceptible pigs on the transmission of Streptococcus suis (S. suis. In three replicate experiments, S. suis-free pigs were housed in boxes either in pairs (25 pairs or alone (15 pigs. The distance between the boxes was ±1 m. At 7 weeks of age, one pig of each pair was inoculated intranasally with S. suis serotype 9; the other pigs were exposed to S. suis by either direct (pairs or indirect contact (individually housed pigs. Tonsillar brush and saliva swab samples from all pigs were collected regularly for 4 weeks post inoculation to monitor colonization with S. suis. All inoculated pigs became infected, and their pen mates became colonized within 2 days. Thirteen indirectly exposed pigs became positive within 7-25 days after exposure. The rate of direct transmission βdir was estimated to be 3.58 per pig per day (95% CI: 2.29-5.60. The rate of indirect transmission increased in time, depending on the cumulative number of days pigs tested positive for the presence of S. suis. The estimate β'ind was 0.001 (95% CI: 0.0006-0.0017 new infections per pig per day for each day that an infected pig was tested positive for S. suis. We conclude that prevention of direct contact reduces the rate at which susceptible pigs become colonized. Simulation studies using these parameters showed, however, that such intervention measure would not limit S. suis serotype 9 spread in a commercial pig farm to a relevant extent, implying that spatial separation of groups op pigs within a compartment would not be effective on a farm.

Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization

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Thi Hoa

2011-07-01

Full Text Available Abstract Background Streptococcus suis is a zoonotic pathogen that causes infections in young piglets. S. suis is a heterogeneous species. Thirty-three different capsular serotypes have been described, that differ in virulence between as well as within serotypes. Results In this study, the correlation between gene content, serotype, phenotype and virulence among 55 S. suis strains was studied using Comparative Genome Hybridization (CGH. Clustering of CGH data divided S. suis isolates into two clusters, A and B. Cluster A isolates could be discriminated from cluster B isolates based on the protein expression of extracellular factor (EF. Cluster A contained serotype 1 and 2 isolates that were correlated with virulence. Cluster B mainly contained serotype 7 and 9 isolates. Genetic similarity was observed between serotype 7 and serotype 2 isolates that do not express muramidase released protein (MRP and EF (MRP-EF-, suggesting these isolates originated from a common founder. Profiles of 25 putative virulence-associated genes of S. suis were determined among the 55 isolates. Presence of all 25 genes was shown for cluster A isolates, whereas cluster B isolates lacked one or more putative virulence genes. Divergence of S. suis isolates was further studied based on the presence of 39 regions of difference. Conservation of genes was evaluated by the definition of a core genome that contained 78% of all ORFs in P1/7. Conclusions In conclusion, we show that CGH is a valuable method to study distribution of genes or gene clusters among isolates in detail, yielding information on genetic similarity, and virulence traits of S. suis isolates.

Clearance of Streptococcus suis in Stomach Contents of Differently Fed Growing Pigs

OpenAIRE

Franziska Warneboldt; Saara J. Sander; Andreas Beineke; Peter Valentin-Weigand; Josef Kamphues; Christoph Georg Baums

2016-01-01

Streptococcus (S.) suis translocates across the intestinal barrier of piglets after intraintestinal application. Based on these findings, an oro-gastrointestinal infection route has been proposed. Thus, the objective of this study was to investigate the survival of S. suis in the porcine stomach. Whereas surviving bacteria of S. suis serotypes 2 and 9 were not detectable after 60 min of incubation in stomach contents with a comparatively high gastric pH of 5 due to feeding of fine pellets, th...

Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

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Franco F. Calderaro

Full Text Available Abstract: Streptococcus suis is one of most important pathogens in the swine industry worldwide. Despite its importance, studies of S. suis characterization in South America are still rare. This study evaluates S. suis isolates from distinct Brazilian states, from 1999 to 2004, and its molecular and serological characterization. A total of 174 isolates were studied. S. suis identification was confirmed by PCR and isolates were further serotyped and genotyped by SE-AFLP and amplification of virulence markers. Serotype 1, 2, 3, 4, 7, 18, 22 and 32 were identified among the studied isolates, and only 4% were characterized as non-typeable. The mrp+/epf+/sly+ genotype was the most frequent. The SE-AFLP analysis resulted in 29 patterns distributed in three main clusters with over 65% of genetic similarity. Isolates presented a slight tendency to cluster according to serotype and origin; however, no further correlation with virulence genotypes was observed.

Isolation, Characterization and Biological Properties of Membrane Vesicles Produced by the Swine Pathogen Streptococcus suis.

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Bruno Haas

Full Text Available Streptococcus suis, more particularly serotype 2, is a major swine pathogen and an emerging zoonotic agent worldwide that mainly causes meningitis, septicemia, endocarditis, and pneumonia. Although several potential virulence factors produced by S. suis have been identified in the last decade, the pathogenesis of S. suis infections is still not fully understood. In the present study, we showed that S. suis produces membrane vesicles (MVs that range in diameter from 13 to 130 nm and that appear to be coated by capsular material. A proteomic analysis of the MVs revealed that they contain 46 proteins, 9 of which are considered as proven or suspected virulence factors. Biological assays confirmed that S. suis MVs possess active subtilisin-like protease (SspA and DNase (SsnA. S. suis MVs degraded neutrophil extracellular traps, a property that may contribute to the ability of the bacterium to escape the host defense response. MVs also activated the nuclear factor-kappa B (NF-κB signaling pathway in both monocytes and macrophages, inducing the secretion of pro-inflammatory cytokines, which may in turn contribute to increase the permeability of the blood brain barrier. The present study brought evidence that S. suis MVs may play a role as a virulence factor in the pathogenesis of S. suis infections, and given their composition be an excellent candidate for vaccine development.

Epidemiological relationship of human and swine Streptococcus suis isolates.

Science.gov (United States)

Tarradas, C; Luque, I; de Andrés, D; Abdel-Aziz Shahein, Y E; Pons, P; González, F; Borge, C; Perea, A

2001-06-01

Two cases of meningitis due to Streptococcus suis in humans are reported here. A butcher and an abattoir worker were referred to a health centre in Castellón (Spain) with fever and symptoms of meningitis. After adequate treatment, a slight hipoacusia persisted as sequelae in both cases. Colonies of S. suis group R, serotype 2 and phenotype MRP+EF+ were isolated from cerebroespinal fluid. Epidemiological studies showed that both workers had in common the handling of pork meat of slaughtered healthy pigs from three closed farms. A study of the tonsils from apparently healthy, slaughtered pigs was carried out. A total of 234 tonsillar samples were obtained and 81 strains of S. suis were isolated from them. Serotype 2 appeared to be the most frequent (50.6%), and the analysis for phenotype showed a high percentage of tonsillar strains with the phenotype MRP+EF+ (35.9%). The humans and 28 tonsillar swine strains showed a similar profile (S. suis group R, serotype 2 and phenotype MRP+EF+). A total of 26 of the swine isolates were analysed by ribotyping using EcoRI. The human strains showed the same six-band hybridization pattern that shared five bands with the pattern most frequently shown by most of the tonsillar N. suis group R, serotype 2 and phenotype MRP+EF+ strains, differing only in the lightest, faintest band which was slightly less anodical in human (> or = 1.8 kb) than in swine (approximately 1.8 kb). From these results, both groups of strains, humans and porcine, showed differences; how can these differences in the pattern of ribotyping be explained if they should have the same origin? Is it possible that they have undergone an adaptation to the new host or perhaps the modification is due to other unknown causes? Further studies in this area are required in order to answer these questions.

Diagnosis Infeksi Streptococcus suis serotipe-2 pada Babi Secara Serologi dengan Muramidase Released Protein (SEROLOGICALLY DIAGNOSE OF STREPTOCOCCUS SUIS SEROTYPE-2 INFECTION IN PIGS BASED ON MURAMIDASE RELEASED PROTEIN

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Siti Isrina Oktavia Salasia

2016-01-01

Full Text Available Streptococcus suis is a bacterial pathogen causing disease of pigs that characterized by meningitis,bronchopneumonia, arthritis, pericarditis, polyserositis and septicaemia. S. suis especially serotype 2 caninfect human (zoonotic with a special symptom of meningitis. The aim of this research was to detect S.suis infection based on muramidase released protein (MRP, as an important virulence marker of S. suis.S. suis serotype 2 strain P171 with phenotype of MRP+EF+ was used in this research. The MRP antigen wasextracted using lysozyme and separated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE. Balb/c mice were imunized with 136 kDa MRP to produce antibody against MRP. Theantibody was evaluated by using enzyme linkage immunosorbent assay (ELISA. The results of the researchshowed that the antibody against MRP with molecular weight of 136 kDa could be produced on Balb/Cmice with the highest absorbance of 3,889 and could be used to detect field sera from infected pigs with200x dilution using ELISA antigen capture. Antibody against MRP could detect serologically of S. suisinfection in pigs in Papua with 50% seropositivy by using ELISA antigen capture and 40% by using dot blot.

Interaction of Fibrinogen and Muramidase-released Protein Promotes the Development of Streptococcus suis Meningitis

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Junping eWang

2015-09-01

Full Text Available Muramidase-released protein (MRP is as an important virulence marker of Streptococcus suis (S. suis serotype 2. Our previous works have shown that MRP can bind human fibrinogen (hFg; however, the function of this interaction in S.suis meningitis is not known. In this study, we found that the deletion of mrp significantly impairs the hFg-mediated adherence and traversal ability of S. suis across human cerebral microvascular endothelial cells (hCMEC/D3. Measurement of the permeability to Lucifer yellow in vitro and Evans blue extravasation in vivo show that the MRP-hFg interaction significantly increases the permeability of the blood-brain barrier (BBB. In the mouse meningitis model, wild type S. suis caused higher bacterial loads in the brain and more severe histopathological signs of meningitis than the mrp mutant at day 3 post-infection. Western blot analysis and immunofluorescence observations reveal that the MRP-hFg interaction can destroy the cell adherens junction protein p120-catenin of hCMEC/D3. These results indicate that the MRP-hFg interaction is important in the development of S. suis meningitis.

Sanitary Landfill Supplemental Test Final Report

International Nuclear Information System (INIS)

Altman, D.J.

1999-01-01

This report summarizes the performance of the Sanitary Landfill Supplemental Test data, an evaluation of applicability, conclusions, recommendations, and related information for implementation of this remediation technology at the SRS Sanitary Landfill

Streptococcus suis Meningitis: First Case Reported in Quebec

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Sophie Michaud

1996-01-01

Full Text Available Very few Streptococcus suis infections in humans have been reported in Canada, although the condition is frequent in pigs. Meningitis, often accompanied by severe hearing loss, is the most common clinical manifestation. The disease is an occupational illness affecting persons in contact with pigs and may be underdiagnosed because of misidentification of the responsible bacterium. Since Quebec is the leading province for swine production in Canada, physicians and microbiologists should be aware of this infection, especially when a streptococcal meningitis is diagnosed in swine workers. The first case of S suis type 2 meningitis reported in Quebec is described.

Monographs in Tang Official Historiography: Perspectives from the Technical Treatises of the History of Sui (Sui shu)

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Morgan, Daniel Patrick; Chaussende, Damien

2018-01-01

Finished in 656 as part of a larger project, the ten ‘treatises’ (zhi) of the current History of Sui (Sui shu) provide us with histories of such fields of technical knowledge as astronomy, astrology, omenology, economics, law, geography, metrology, and library science covering the five divided and short-lived dynasties, 502–618 CE, that were the predecessors of the Tang. This edited volume brings together young experts in these fields to reflect upon the way that their history has been writte...

Evaluation of Galleria mellonella larvae for studying the virulence of Streptococcus suis

NARCIS (Netherlands)

Velikova, Nadya; Kavanagh, Kevin; Wells, Jerry M.

2016-01-01

Background: Streptococcus suis is an encapsulated Gram-positive bacterium and the leading cause of sepsis and meningitis in young pigs, resulting in considerable economic losses in the porcine industry. S. suis is considered an emerging zoonotic agent with increasing numbers of human cases over the

Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.

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Kristien De Puysseleyr

Full Text Available Pigs are the natural host for Chlamydia suis, a pathogen which is phylogenetically highly related to the human pathogen C. trachomatis. Chlamydia suis infections are generally treated with tetracyclines. In 1998, tetracyline resistant C. suis strains emerged on U.S. pig farms and they are currently present in the Belgian, Cypriote, German, Israeli, Italian and Swiss pig industry. Infections with tetracycline resistant C. suis strains are mainly associated with severe reproductive failure leading to marked economical loss. We developed a sensitive and specific TaqMan probe-based C. suis real-time PCR for examining clinical samples of both pigs and humans. The analytical sensitivity of the real-time PCR is 10 rDNA copies/reaction without cross-amplifying DNA of other Chlamydia species. The PCR was successfully validated using conjunctival, pharyngeal and stool samples of slaughterhouse employees, as well as porcine samples from two farms with evidence of reproductive failure and one farm without clinical disease. Chlamydia suis was only detected in diseased pigs and in the eyes of humans. Positive humans had no clinical complaints. PCR results were confirmed by culture in McCoy cells. In addition, Chlamydia suis isolates were also examined by the tet(C PCR, designed for demonstrating the tetracycline resistance gene tet(C. The tet(C gene was only present in porcine C. suis isolates.

Streptococcus suis meningitis in the Netherlands

NARCIS (Netherlands)

van de Beek, Diederik; Spanjaard, Lodewijk; de Gans, Jan

2008-01-01

We present four patients with Streptococcus suis meningitis identified during a 3.5-year prospective surveillance study in the Netherlands. All cases were associated with exposure to pigs. Patients presented with classic symptoms and signs of bacterial meningitis. Outcome was characterized by severe

Molecular identification of Trichuris vulpis and Trichuris suis isolated from different hosts.

Science.gov (United States)

Cutillas, Cristina; de Rojas, Manuel; Ariza, Concepción; Ubeda, José Manuel; Guevara, Diego

2007-01-01

Trichuris suis was isolated from the cecum of two different hosts (Sus scrofa domestica -- swine and Sus scrofa scrofa -- wild boar) and Trichuris vulpis from dogs in Sevilla, Spain. Genomic DNA was isolated and internal transcribed spacers (ITS)1-5.8S-ITS2 segment from the ribosomal DNA (rDNA) was amplified and sequenced using polymerase chain reaction techniques. The sequence of T. suis from both hosts was 1,396 bp in length while that of T. vulpis was 1,044 bp. ITS1 of both populations isolated of T. suis was 661 nucleotides in length, while the ITS2 was 534 nucleotides in length. Furthermore, the ITS1 of T. vulpis was 410 nucleotides in length, while the ITS2 was 433 nucleotides in length. One hundred fifty-four nucleotides were observed along the 5.8S gene of T. suis and T. vulpis. Intraindividual and intraspecific variations were detected in the rDNA of both species. The presence of microsatellites was observed in all the individuals assayed. Sequence analysis of the ITSs and the 5.8S gene has demonstrated no sequence differences between T. suis isolated from both hosts (S. scrofa domestica -- swine and S. scrofa scrofa -- wild boar). Nevertheless, clear differences were detected between the ITS1 and ITS2 of T. suis and T. vulpis. Furthermore, a comparative molecular analysis between both species and the previously published ITS1-5.8S-ITS2 sequence data of Trichuris ovis, Trichuris leporis, Trichuris muris, Trichuris arvicolae, and Trichuris skrjabini was carried out. A common homology zone was detected in the ITS1 sequence of all species of trichurids.

Parasite population dynamics in pigs infected with Trichuris suis and Oesophagostomum dentatum

DEFF Research Database (Denmark)

Petersen, Heidi Huus; Andreasen, Annette; Kringel, Helene

2014-01-01

The aim of the present study was to investigate the population dynamics and potential interactions between Trichuris suis and Oesophagostomum dentatum in experimentally co-infected pigs, by quantification of parasite parameters such as egg excretion, worm recovery and worm location. Forty......-eight helminth naïve pigs were allocated into four groups. Group O was inoculated with 20 O. dentatum L3/kg/day and group T with 10 T. suis eggs/kg/day. Group OT was inoculated with both 20 O. dentatum L3/kg/day and 10 T. suis eggs/kg/day, while Group C was kept as an uninfected control group. All inoculations...

Impact of Sub-Inhibitory Concentrations of Amoxicillin on Streptococcus suis Capsule Gene Expression and Inflammatory Potential

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Bruno Haas

2016-04-01

Full Text Available Streptococcus suis is an important swine pathogen and emerging zoonotic agent worldwide causing meningitis, endocarditis, arthritis and septicemia. Among the 29 serotypes identified to date, serotype 2 is mostly isolated from diseased pigs. Although several virulence mechanisms have been characterized in S. suis, the pathogenesis of S. suis infections remains only partially understood. This study focuses on the response of S. suis P1/7 to sub-inhibitory concentrations of amoxicillin. First, capsule expression was monitored by qRT-PCR when S. suis was cultivated in the presence of amoxicillin. Then, the pro-inflammatory potential of S. suis P1/7 culture supernatants or whole cells conditioned with amoxicillin was evaluated by monitoring the activation of the NF-κB pathway in monocytes and quantifying pro-inflammatory cytokines secreted by macrophages. It was found that amoxicillin decreased capsule expression in S. suis. Moreover, conditioning the bacterium with sub-inhibitory concentrations of amoxicillin caused an increased activation of the NF-κB pathway in monocytes following exposure to bacterial culture supernatants and to a lesser extent to whole bacterial cells. This was associated with an increased secretion of pro-inflammatory cytokines (CXCL8, IL-6, IL-1β by macrophages. This study identified a new mechanism by which S. suis may increase its inflammatory potential in the presence of sub-inhibitory concentrations of amoxicillin, a cell wall-active antibiotic, thus challenging its use for preventive treatments or as growth factor.

Streptococcus suis Interactions with the Murine Macrophage Cell Line J774: Adhesion and Cytotoxicity

OpenAIRE

Segura, Mariela; Gottschalk, Marcelo

2002-01-01

Streptococcus suis capsular type 2 is an important etiological agent of swine meningitis, and it is also a zoonotic agent. Since one hypothesis of the pathogenesis of S. suis infection is that bacteria enter the bloodstream and invade the meninges and other tissues in close association with mononuclear phagocytes, the objective of the present study was to evaluate the capacity of S. suis type 2 to adhere to macrophages. An enzyme-linked immunosorbent assay technique was standardized to simply...

Study on bioactive compounds of in vitro cultured Calculus Suis and natural Calculus Bovis.

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Wan, Tien-Chun; Cheng, Fu-Yuan; Liu, Yu-Tse; Lin, Liang-Chuan; Sakata, Ryoichi

2009-12-01

The purpose of the study was to investigate bioactive compounds of in vitro cultured Calculus Suis and natural Calculus Bovis obtained as valuable by-products from animals used for meat production. The results showed that the components of natural Calculus Bovis were rich in bilirubin and biliverdin and had higher content of essential amino acids. The major amino acids of in vitro cultured Calculus Suis were identified as glycine, alanine, glutamic acid and aspartic acid, and those for natural Calculus Bovis were found to be glutamic acid, aspartic acid, proline, and arginine. The methionine and cysteine contents of precursors for glutathione in natural Calculus Bovis were significantly higher than those of in vitro cultured Calculus Suis. The mineral contents of zinc, iron and manganese of natural Calculus Bovis were significantly higher than those of in vitro cultured Calculus Suis. The major bile acids in both products were cholic acid and dehydrocholic acid, respectively. The chenodeoxycholic and ursodeoxycholic acid content of in vitro cultured Calculus Suis was significantly higher than that of natural Calculus Bovis.

21 CFR 1210.14 - Sanitary inspection of plants.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Sanitary inspection of plants. 1210.14 Section... FEDERAL IMPORT MILK ACT Inspection and Testing § 1210.14 Sanitary inspection of plants. The sanitary conditions of any plant handling milk or cream any part of which is to be shipped or transported into the...

Capsular Sialyltransferase Specificity Mediates Different Phenotypes in Streptococcus suis and Group B Streptococcus

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David Roy

2018-04-01

Full Text Available The capsular polysaccharide (CPS represents a key virulence factor for most encapsulated streptococci. Streptococcus suis and Group B Streptococcus (GBS are both well-encapsulated pathogens of clinical importance in veterinary and/or human medicine and responsible for invasive systemic diseases. S. suis and GBS are the only Gram-positive bacteria which express a sialylated CPS at their surface. An important difference between these two sialylated CPSs is the linkage between the side-chain terminal galactose and sialic acid, being α-2,6 for S. suis but α-2,3 for GBS. It is still unclear how sialic acid may affect CPS production and, consequently, the pathogenesis of the disease caused by these two bacterial pathogens. Here, we investigated the role of sialic acid and the putative effect of sialic acid linkage modification in CPS synthesis using inter-species allelic exchange mutagenesis. To this aim, a new molecular biogenetic approach to express CPS with modified sialic acid linkage was developed. We showed that sialic acid (and its α-2,6 linkage is crucial for S. suis CPS synthesis, whereas for GBS, CPS synthesis may occur in presence of an α-2,6 sialyltransferase or in absence of sialic acid moiety. To evaluate the effect of the CPS composition/structure on sialyltransferase activity, two distinct capsular serotypes within each bacterial species were compared (S. suis serotypes 2 and 14 and GBS serotypes III and V. It was demonstrated that the observed differences in sialyltransferase activity and specificity between S. suis and GBS were serotype unrestricted. This is the first time that a study investigates the interspecies exchange of capsular sialyltransferase genes in Gram-positive bacteria. The obtained mutants represent novel tools that could be used to further investigate the immunomodulatory properties of sialylated CPSs. Finally, in spite of common CPS structural characteristics and similarities in the cps loci, sialic acid exerts

9 CFR 416.4 - Sanitary operations.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Sanitary operations. 416.4 Section 416... SANITATION § 416.4 Sanitary operations. (a) All food-contact surfaces, including food-contact surfaces of... compounds, sanitizing agents, processing aids, and other chemicals used by an establishment must be safe and...

A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador.

Science.gov (United States)

Meekums, Hayley; Hawash, Mohamed B F; Sparks, Alexandra M; Oviedo, Yisela; Sandoval, Carlos; Chico, Martha E; Stothard, J Russell; Cooper, Philip J; Nejsum, Peter; Betson, Martha

2015-03-19

Since the nematodes Trichuris trichiura and T. suis are morphologically indistinguishable, genetic analysis is required to assess epidemiological cross-over between people and pigs. This study aimed to clarify the transmission biology of trichuriasis in Ecuador. Adult Trichuris worms were collected during a parasitological survey of 132 people and 46 pigs in Esmeraldas Province, Ecuador. Morphometric analysis of 49 pig worms and 64 human worms revealed significant variation. In discriminant analysis morphometric characteristics correctly classified male worms according to host species. In PCR-RFLP analysis of the ribosomal Internal Transcribed Spacer (ITS-2) and 18S DNA (59 pig worms and 82 human worms), nearly all Trichuris exhibited expected restriction patterns. However, two pig-derived worms showed a "heterozygous-type" ITS-2 pattern, with one also having a "heterozygous-type" 18S pattern. Phylogenetic analysis of the mitochondrial large ribosomal subunit partitioned worms by host species. Notably, some Ecuadorian T. suis clustered with porcine Trichuris from USA and Denmark and some with Chinese T. suis. This is the first study in Latin America to genetically analyse Trichuris parasites. Although T. trichiura does not appear to be zoonotic in Ecuador, there is evidence of genetic exchange between T. trichiura and T. suis warranting more detailed genetic sampling.

Dai bisogni di salute al consumo di risorse sanitarie in provincia di Bergamo: profilo degli indicatori di bisogno, domanda, offerta

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A. Zucchi

2003-05-01

Full Text Available

Introduzione: la complessità interpretativa dei dati relativi ad alcuni fenomeni sanitari a livello territoriale impone la necessità di uno strumento semplice di lettura “epidemiologica”; tale lettura si fonda sull’utilizzo di una serie di “indicatori”, fondamenta di un processo dinamico di monitoraggio temporale e spaziale in grado di descrivere sinteticamente fenomeni di per sé complessi, al fine di fornire al decisore un supporto sintetico ma scientificamente solido.

Obiettivi: istituzione di un sistema di monitoraggio sanitario community-based; definizione di indicatori sanitari comparabili tramite un esame critico dei dati esistenti; sviluppo di metodologie per la raccolta e la validazione dei dati; supporto alla creazione di un sistema efficace e affidabile di trasferimento e scambio dei dati e degli indicatori stessi con uso spinto della tecnologia telematica; elaborazione di metodi e di strumenti necessari alla produzione di analisi e relazioni.

Materiali e metodi: gli indicatori analizzati fanno riferimento
all’elenco definito dal decreto ministeriale n. 27 del 12 dicembre 2001 “sistema di garanzie per il monitoraggio dell’assistenza sanitaria”. A questo elenco ne sono stati aggiunti altri, per un totale di 127, divisi in grandi capitoli tematici: assistenza sanitaria, assistenza distrettuale, assistenza ospedaliera, indicatori stato di salute, consumo di risorse sanitarie (cui è stato dato particolare enfasi, indicatori di domanda/accessibilità. Per definire infine un
profilo di sintesi di questi grandi capitoli e delle relative
eterogeneità territoriali si è successivamente applicata la tecnica statistica dello “z-score”.
Risultati: le differenze emerse a livello distrettuale hanno permesso di disegnare una ‘mappa’ degli indicatori sanitari in provincia di Bergamo.

Conclusioni:i risultati di

[Sanitary education of workers of bakeries and pastry enterprises].

Science.gov (United States)

Krasil'shchikov, M I; Nemets, M G; Novikova, O V; Gavrilenko, E V; Osipova, E M; Osinova, T I

2000-01-01

The paper presents experience with sanitary education of workers from food industrial enterprises. The study revealed a number of disadvantages of the methodological aspect of sanitary education of these workers. A sanitary education programme has been worked out for workers engaged in food and food processing industries (production of bread, baked goods, and confectionery) and approved by the State Sanitary and Epidemiological Surveillance Department), Ministry of Health of Russia on March 1999.

Bacteria-induced egg hatching differs for Trichuris muris and Trichuris suis

DEFF Research Database (Denmark)

Vejzagic, Nermina; Adelfio, Roberto; Keiser, Jennifer

2015-01-01

BACKGROUND: Eggs of the porcine whipworm Trichuris suis are currently explored in human clinical trials as a treatment of immune-mediated diseases. In this context, only the infective, embryonated eggs, constitute the Active Pharmaceutical Ingredient (API). The rodent whipworm, Trichuris muris...... is commonly used as a laboratory model to study Trichuris biology. The embryonated eggs (containing a fully developed larva) are biologically active and will invade the large intestinal mucosa of the host. This study aims to assess the in vitro hatching of T. muris and T. suis eggs in various bacterial...... cultures as a measure for their biological activity. METHODS: Eggs of T. muris and T. suis were incubated with Escherichia coli strain (BL-21) at three concentrations in a slightly modified in vitro egg hatching assay previously developed for T. muris. Additionally, E. coli strains (M15, SG13009, PMC103...

Antimicrobial activity of nisin against the swine pathogen Streptococcus suis and its synergistic interaction with antibiotics.

Science.gov (United States)

Lebel, Geneviève; Piché, Fanny; Frenette, Michel; Gottschalk, Marcelo; Grenier, Daniel

2013-12-01

Streptococcus suis serotype 2 is known to cause severe infections in pigs, including meningitis, endocarditis and pneumonia. Furthermore, this bacterium is considered an emerging zoonotic agent. Recently, increased antibiotic resistance in S. suis has been reported worldwide. The objective of this study was to evaluate the potential of nisin, a bacteriocin of the lantibiotic class, as an antibacterial agent against the pathogen S. suis serotype 2. In addition, the synergistic activity of nisin in combination with conventional antibiotics was assessed. Using a plate assay, the nisin-producing strain Lactococcus lactis ATCC 11454 proved to be capable of inhibiting the growth of S. suis (n=18) belonging to either sequence type (ST)1, ST25, or ST28. In a microdilution broth assay, the minimum inhibitory concentration (MIC) of purified nisin ranged between 1.25 and 5 μg/mL while the minimum bactericidal concentration (MBC) was between 5 and 10 μg/mL toward S. suis. The use of a capsule-deficient mutant of S. suis indicated that the presence of this polysaccharidic structure has no marked impact on susceptibility to nisin. Following treatment of S. suis with nisin, transmission electron microscopy observations revealed lysis of bacteria resulting from breakdown of the cell membrane. A time-killing curve showed a rapid bactericidal activity of nisin. Lastly, synergistic effects of nisin were observed in combination with several antibiotics, including penicillin, amoxicillin, tetracycline, streptomycin and ceftiofur. This study brought clear evidence supporting the potential of nisin for the prevention and treatment of S. suis infections in pigs. Copyright © 2013 Elsevier Inc. All rights reserved.

Effectiveness of REDD programs in the protection of sui generis indigenous rights

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Paloma Infante M.

2013-06-01

Full Text Available The article focuses on the implementation of the United Nations Reducing Emission from Deforestation and Forest Degradation Program, hereinafter UN REDD and its effectiveness in the protection of sui generis indigenous rights, meaning those different from the property right but derived from it, especially, the rights over the territories, natural resources and environmental services. The paper will cover the interconnection between sui generis indigenous rights and REDD programs including the relevance of land tenure and the sui generis rights derived from it. As we will emphasize, REDD programs are not only focused in the reduction of carbon emissions. In fact, REDD plus programs include as a main objective the sustainable management of forests and the protection to forest dependent communities.

Incidence and presence of virulence factors of Streptococcus suis infection in slaughtered pigs from Chiang Mai, Thailand.

Science.gov (United States)

Padungtod, Pawin; Tharavichitkul, Prasit; Junya, Supansa; Chaisowong, Warangkhana; Kadohira, Mutsuyo; Makino, Souichi; Sthitmatee, Nattawooti

2010-11-01

This study was designed to determine the incidence of Streptococcus suis infection in slaughtered pigs raised in industrial facility and backyard system in Chiang Mai City, Thailand. A total of 90 tonsils and submaxillary salivary gland/lymph node samples from slaughtered pigs raised in industrial facility and 122 samples from slaughtered pigs raised in backyard system were collected. Isolation and identification of S. suis were conducted using standard bacteriological methods. Farm management and risk factor data were collected by a questionnaire. Serotyping and presence of virulence factor genes, epf, mrp and sly, were determined by multiplex PCR assay. The overall incidence of S. suis in this study was 9% (n = 212) and the incidence is significantly higher in districts located at a greater distance south of Chiang Mai City. S. suis serotype 2 was present more in healthy pigs (43%) than ill pigs (10%). Every S. suis isolate carried mrp and sly and ill pigs carried epf (80%) more than healthy pigs (57%). However, the probability of S. suis serotype 2 with epf+ (0.245) detected in healthy pigs was higher than in ill pigs (0.08) indicating people may have a higher risk of being infected with S. suis from healthy than ill pigs.

Trichuris suis and Oesophagostomum dentatum show different sensitivity and accumulation of fenbendazole, albendazole and levamisole in vitro.

Directory of Open Access Journals (Sweden)

Tina V A Hansen

2014-04-01

Full Text Available BACKGROUND: The single-dose benzimidazoles used against Trichuris trichiura infections in humans are not satisfactory. Likewise, the benzimidazole, fenbendazole, has varied efficacy against Trichuris suis whereas Oesophagostomum dentatum is highly sensitive to the drug. The reasons for low treatment efficacy of Trichuris spp. infections are not known. METHODOLOGY: We studied the effect of fenbendazole, albendazole and levamisole on the motility of T. suis and O. dentatum and measured concentrations of the parent drug compounds and metabolites of the benzimidazoles within worms in vitro. The motility and concentrations of drug compounds within worms were compared between species and the maximum specific binding capacity (Bmax of T. suis and O. dentatum towards the benzimidazoles was estimated. Comparisons of drug uptake in living and killed worms were made for both species. PRINCIPAL FINDINGS: The motility of T. suis was generally less decreased than the motility of O. dentatum when incubated in benzimidazoles, but was more decreased when incubated in levamisole. The Bmax were significantly lower for T. suis (106.6, and 612.7 pmol/mg dry worm tissue than O. dentatum (395.2, 958.1 pmol/mg dry worm tissue when incubated for 72 hours in fenbendazole and albendazole respectively. The total drug concentrations (pmol/mg dry worm tissue were significantly lower within T. suis than O. dentatum whether killed or alive when incubated in all tested drugs (except in living worms exposed to fenbendazole. Relatively high proportions of the anthelmintic inactive metabolite fenbendazole sulphone was measured within T. suis (6-17.2% as compared to O. dentatum (0.8-0.9%. CONCLUSION/SIGNIFICANCE: The general lower sensitivity of T. suis towards BZs in vitro seems to be related to a lower drug uptake. Furthermore, the relatively high occurrence of fenbendazole sulphone suggests a higher detoxifying capacity of T. suis as compared to O. dentatum.

Trichuris suis and Oesophagostomum dentatum show different sensitivity and accumulation of fenbendazole, albendazole and levamisole in vitro.

Science.gov (United States)

Hansen, Tina V A; Nejsum, Peter; Friis, Christian; Olsen, Annette; Thamsborg, Stig Milan

2014-04-01

The single-dose benzimidazoles used against Trichuris trichiura infections in humans are not satisfactory. Likewise, the benzimidazole, fenbendazole, has varied efficacy against Trichuris suis whereas Oesophagostomum dentatum is highly sensitive to the drug. The reasons for low treatment efficacy of Trichuris spp. infections are not known. We studied the effect of fenbendazole, albendazole and levamisole on the motility of T. suis and O. dentatum and measured concentrations of the parent drug compounds and metabolites of the benzimidazoles within worms in vitro. The motility and concentrations of drug compounds within worms were compared between species and the maximum specific binding capacity (Bmax) of T. suis and O. dentatum towards the benzimidazoles was estimated. Comparisons of drug uptake in living and killed worms were made for both species. The motility of T. suis was generally less decreased than the motility of O. dentatum when incubated in benzimidazoles, but was more decreased when incubated in levamisole. The Bmax were significantly lower for T. suis (106.6, and 612.7 pmol/mg dry worm tissue) than O. dentatum (395.2, 958.1 pmol/mg dry worm tissue) when incubated for 72 hours in fenbendazole and albendazole respectively. The total drug concentrations (pmol/mg dry worm tissue) were significantly lower within T. suis than O. dentatum whether killed or alive when incubated in all tested drugs (except in living worms exposed to fenbendazole). Relatively high proportions of the anthelmintic inactive metabolite fenbendazole sulphone was measured within T. suis (6-17.2%) as compared to O. dentatum (0.8-0.9%). The general lower sensitivity of T. suis towards BZs in vitro seems to be related to a lower drug uptake. Furthermore, the relatively high occurrence of fenbendazole sulphone suggests a higher detoxifying capacity of T. suis as compared to O. dentatum.

Natural gas prices in Italy. Tariffs geographical distribution; Metano. Paese che vai, prezzo che trovi

Energy Technology Data Exchange (ETDEWEB)

Marrocchelli, A. [ENEA, Rome (Italy). Centro Ricerche Casaccia

2000-11-01

The annual report on services and activity carries at some evaluations of data concerned the natural gas market: total consumption, costs and prices in Italy and comparative evaluations with other european countries. [Italian] La relazione annuale sullo stato dei servizi e sull'attivita' svolta, presentata dal Presidente dell'Autorita' per l'energia elettrica e il gas, porta ad alcune considerazioni sui dati che riguardano il mercato del gas naturale: i consumi totali, i costi e i prezzi in Italia, il confronto con i costi e i prezzi praticati negli altri paesi europei.

Cold Vacuum Drying facility sanitary sewage collection system design description

International Nuclear Information System (INIS)

PITKOFF, C.C.

1999-01-01

This document describes the Cold Vacuum Drying Facility (CVDF) sanitary sewage collection system. The sanitary sewage collection system provides collection and storage of effluents and raw sewage from the CVDF to support the cold vacuum drying process. This system is comprised of a sanitary sewage holding tank and pipes for collection and transport of effluents to the sanitary sewage holding tank

Pathway of oxfendazole from the host into the worm: Trichuris suis in pigs

Directory of Open Access Journals (Sweden)

Tina V.A. Hansen

2017-12-01

Full Text Available It is well known that the efficacy of a single oral dose of benzimidazoles against Trichuris spp. infections in humans and animals is poor, but is currently still used in control programmes against human trichuriasis. However, the route of the benzimidazoles from the treated host to Trichuris remains unknown. As parts of adult Trichuris are situated intracellularly in the caecum, they might be exposed to anthelmintic drugs in the intestinal content as well as the mucosa. In this study, the pathway of oxfendazole and its metabolites was explored using a T. suis-pig infection model, by simultaneously measuring drug concentrations within the worms and the caecal mucosa, caecal tissue, caecal content and plasma of pigs over time after a single oral dose of 5 mg/kg oxfendazole. Additionally, for comparison to the in vivo study, drug uptake and metabolism of oxfendazole by T. suis was examined after in vitro incubation. Oxfendazole and metabolites were quantified by High Performance Liquid Chromatography.Multivariate linear regression analysis showed a strong and highly significant association between OFZ concentrations within T. suis and in plasma, along with a weaker association between OFZ concentrations in caecal tissue/mucosa and T. suis, suggesting that oxfendazole reaches T. suis after absorption from the gastrointestinal tract and enters the worms by the blood-enterocyte pathway. The fenbendazole sulfone level in T. suis was highly affected by the concentrations in plasma. In addition, correlations between drug concentrations in the host compartments, were generally highest for this metabolite. In comparison to oxfendazole, the correlation between plasma and content was particularly high for this metabolite, suggesting a high level of drug movement between these compartments and the possible involvement of the enterohepatic circulation. Keywords: Trichuris, Benzimidazole, Drug efficacy, Drug pathway

Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies

Science.gov (United States)

Mathieu-Denoncourt, Annabelle; Letendre, Corinne; Auger, Jean-Philippe; Segura, Mariela; Aragon, Virginia; Lacouture, Sonia; Gottschalk, Marcelo

2018-01-01

Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infection with tracheal epithelial cells or primary alveolar macrophages (PAMs), it was possible to determine the interaction between S. suis serotype 2 and H. parasuis strains with different level of virulence. Within H. parasuis strains, the low-virulence F9 strain showed higher adhesion levels to respiratory epithelial cells and greater association levels to PAMs than the high-virulence Nagasaki strain. Accordingly, the low-virulence F9 strain induced, in general, higher levels of pro-inflammatory cytokines than the virulent Nagasaki strain from both cell types. In general, S. suis adhesion levels to respiratory epithelial cells were similar to H. parasuis Nagasaki strain. Yet, S. suis strains induced a significantly lower level of pro-inflammatory cytokine expression from epithelial cells and PAMs than those observed with both H. parasuis strains. Finally, this study has shown that, overall and under the conditions used in the present study, S. suis and H. parasuis have limited in vitro interactions between them and use probably different host receptors, regardless to their level of virulence. PMID:29316613

25 CFR 700.55 - Decent, safe, and sanitary dwelling.

Science.gov (United States)

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false Decent, safe, and sanitary dwelling. 700.55 Section 700... PROCEDURES General Policies and Instructions Definitions § 700.55 Decent, safe, and sanitary dwelling. (a) General. The term decent, safe, and sanitary dwelling means a dwelling which— (1) Meets applicable federal...

First human case report of sepsis due to infection with Streptococcus suis serotype 31 in Thailand.

Science.gov (United States)

Hatrongjit, Rujirat; Kerdsin, Anusak; Gottschalk, Marcelo; Takeuchi, Dan; Hamada, Shigeyuki; Oishi, Kazunori; Akeda, Yukihiro

2015-09-30

Streptococcus suis is a zoonotic pathogen that causes invasive infections in humans and pigs. It has been reported that S. suis infection in humans is mostly caused by serotype 2. However, human cases caused by other serotypes have rarely been reported. This is the first report of a human case of infection with S. suis serotype 31 in Thailand. A 55-year-old male alcohol misuser with liver cirrhosis was admitted with sepsis to a hospital in the Central Region of Thailand. He had consumed a homemade, raw pork product prior to the onset of illness. He was alive after treatment with ceftriaxone and no complication occurred. An isolate from blood culture at the hospital was suspected as viridans group Streptococcus. It was confirmed at a reference laboratory as S. suis serotype 31 by biochemical tests, 16S rDNA sequencing, and multiplex polymerase chain reaction for serotyping, but it was untypable by the co-agglutination test with antisera against recognized S. suis serotypes, suggesting loss of capsular material. The absence of a capsule was confirmed by transmission electron microscopy. The isolate was confirmed to be sequence type 221, with 13 putative virulence genes that are usually found in serotype 2 strains. We should be aware of the emergence of S. suis infections caused by uncommon serotypes in patients with predisposing conditions. Laboratory capacity to identify S. suis in the hospital is needed in developing countries, which can contribute to enhanced surveillance, epidemiological control, and prevention strategies in the prevalent area.

Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22 and 26: Streptococcus parasuis sp. nov.

Science.gov (United States)

Nomoto, R; Maruyama, F; Ishida, S; Tohya, M; Sekizaki, T; Osawa, Ro

2015-02-01

In order to clarify the taxonomic position of serotypes 20, 22 and 26 of Streptococcus suis, biochemical and molecular genetic studies were performed on isolates (SUT-7, SUT-286(T), SUT-319, SUT-328 and SUT-380) reacted with specific antisera of serotypes 20, 22 or 26 from the saliva of healthy pigs as well as reference strains of serotypes 20, 22 and 26. Comparative recN gene sequencing showed high genetic relatedness among our isolates, but marked differences from the type strain S. suis NCTC 10234(T), i.e. 74.8-75.7 % sequence similarity. The genomic relatedness between the isolates and other strains of species of the genus Streptococcus, including S. suis, was calculated using the average nucleotide identity values of whole genome sequences, which indicated that serotypes 20, 22 and 26 should be removed taxonomically from S. suis and treated as a novel genomic species. Comparative sequence analysis revealed 99.0-100 % sequence similarities for the 16S rRNA genes between the reference strains of serotypes 20, 22 and 26, and our isolates. Isolate STU-286(T) had relatively high 16S rRNA gene sequence similarity with S. suis NCTC 10234(T) (98.8 %). SUT-286(T) could be distinguished from S. suis and other closely related species of the genus Streptococcus using biochemical tests. Due to its phylogenetic and phenotypic similarities to S. suis we propose naming the novel species Streptococcus parasuis sp. nov., with SUT-286(T) ( = JCM 30273(T) = DSM 29126(T)) as the type strain. © 2015 IUMS.

21 CFR 1210.11 - Sanitary inspection of dairy farms.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Sanitary inspection of dairy farms. 1210.11... UNDER THE FEDERAL IMPORT MILK ACT Inspection and Testing § 1210.11 Sanitary inspection of dairy farms. The sanitary conditions of any dairy farm producing milk or cream to be shipped or transported into...

Extended semen for artificial insemination in swine as a potential transmission mechanism for infectious Chlamydia suis.

Science.gov (United States)

Hamonic, G; Pasternak, J A; Käser, T; Meurens, F; Wilson, H L

2016-09-01

Although typically unnoticed, Chlamydia infections in swine have been shown to be both widespread and may impact production characteristics and reproductive performance in swine. Serum titers suggest Chlamydia infection within boar studs is common, and infected boars are known to shed chlamydia in their ejaculates. Although the transmission of viruses in chilled extended semen (ES) is well established, the inclusion of antibiotics in commercially available extender is generally believed to limit or preclude the transmission of infectious bacteria. The objective of this study was to evaluate the potential of ES used in artificial insemination to support transmission of the obligate intracellular bacteria Chlamydia suis (C suis) under standard industry conditions. First, the effect of C suis on sperm quality during storage was assessed by flow cytometry. Only concentrations above 5 × 10(5) viable C suis/mL caused significant spermicidal effects which only became evident after 7 days of storage at 17 °C. No significant effect on acrosome reaction was observed using any chlamydial concentration. Next, an in vitro infection model of swine testicular fibroblast cells was established and used to evaluate the effect of chilled storage on C suis viability under variable conditions. Storage in Androhep ES reduced viability by 34.4% at a multiplicity of infection of 1.25, an effect which increased to 53.3% when the multiplicity of infection decreased to 0.1. Interestingly, storage in semen extender alone (SE) or ES with additional antibiotics had no effect on bacterial viability. To rule out a secondary effect on extender resulting from metabolically active sperm, C suis was stored in fresh and expended SE and again no significant effect on bacterial viability was observed. Fluorescent microscopy of C suis in ES shows an association between bacteria and the remaining gel fraction after storage suggesting that the apparent reduction of bacterial viability in the presence

Saccharomyces boulardii administration can inhibit the formation of gastric lymphoid follicles induced by Helicobacter suis infection.

Science.gov (United States)

Yang, Lin; Tian, Zi-Bin; Yu, Ya-Nan; Zhang, Cui-Ping; Li, Xiao-Yu; Mao, Tao; Jing, Xue; Zhao, Wen-Jun; Ding, Xue-Li; Yang, Ruo-Ming; Zhang, Shuai-Qing

2017-01-01

Helicobacter suis has a greater tendency to induce gastric mucosa-associated lymphoid tissue lymphoma compared with other Helicobacter species in humans and animals. Saccharomyces boulardii has been established as an adjunct to H. pylori eradication treatment, but the effect of S. boulardii administration alone on Helicobacter infection remains unclear. Here, we found that S. boulardii administration effectively decreased the bacterial load of H. suis and inhibited the formation of lymphoid follicles in the stomach post-infection. The levels of H. suis-specific immunoglobulin A (IgA) and secretory IgA in the gastric juice and small intestinal secretions and the production of mouse β-defensin-3 in the small intestinal secretions were significantly increased by S. boulardii administration at 12 weeks after H. suis infection. In addition, feeding with S. boulardii inhibited the expression of inflammatory cytokines and lymphoid follicle formation-related factors after H. suis infection. These results suggested that S. boulardii may be useful for the prevention and treatment of Helicobacter infection-related diseases in humans. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies

Directory of Open Access Journals (Sweden)

Annabelle Mathieu-Denoncourt

2018-01-01

Full Text Available Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infection with tracheal epithelial cells or primary alveolar macrophages (PAMs, it was possible to determine the interaction between S. suis serotype 2 and H. parasuis strains with different level of virulence. Within H. parasuis strains, the low-virulence F9 strain showed higher adhesion levels to respiratory epithelial cells and greater association levels to PAMs than the high-virulence Nagasaki strain. Accordingly, the low-virulence F9 strain induced, in general, higher levels of pro-inflammatory cytokines than the virulent Nagasaki strain from both cell types. In general, S. suis adhesion levels to respiratory epithelial cells were similar to H. parasuis Nagasaki strain. Yet, S. suis strains induced a significantly lower level of pro-inflammatory cytokine expression from epithelial cells and PAMs than those observed with both H. parasuis strains. Finally, this study has shown that, overall and under the conditions used in the present study, S. suis and H. parasuis have limited in vitro interactions between them and use probably different host receptors, regardless to their level of virulence.

On Furuto-sui in Chikuho coal field. Chikuho tanden no furutosui ni tsuite

Energy Technology Data Exchange (ETDEWEB)

Yamashita, Akio [Kyushu Univ., Faculty of Engineering, Fukuoka (Japan)

1989-11-30

This paper described the Furuto-sui(goaf water) in Chikuho Coal Field where coal mining was made for 100 years, remaining mined traces in wide range. This is called Furuto and water collected in or passed through Furuto is called Furuto-sui. The Furuto was made by coal layer mining and the distribution is coincident to the geological structure of coal layer. This Furuto structure is shallower to westward direction, deeper to eastward direction and identical to the north-southward direction. The Furuto can be said as the artificially built reservior for ground water. The coal mining was perfectly stopped in 1976 and the Furuto-sui filled in mined cavities. At low ground area, springs or humid areas appeared. Water quality is diverse but the typical ones are the type of Ca-SO{sub 4} of 3-5 pH, type of Na-HCO{sub 3}*SO{sub 4} of 8-9pH, and type of Na-SO{sub 4} of 7 pH. A part of Furuto-sui is utilized as the water for home use but must be utilized much more as water reservior. 12 refs., 9 figs.

Sanitary landfill in situ bioremediation optimization test. Final report

International Nuclear Information System (INIS)

1996-01-01

This work was performed as part of a corrective action plan for the Savannah River Site Sanitary Landfill. This work was performed for the Westinghouse Savannah River Company Environmental Restoration Department as part of final implementation of a groundwater remediation system for the SRS Sanitary Landfill. Primary regulatory surveillance was provided by the South Carolina Department of Health and Environmental Control and the US Environmental Protection Agency (Region IV). The characterization, monitoring and remediation systems in the program generally consisted of a combination of innovative and baseline methods to allow comparison and evaluation. The results of these studies will be used to provide input for the full-scale groundwater remediation system for the SRS Sanitary Landfill. This report summarizes the performance of the Sanitary Landfill In Situ Optimization Test data, an evaluation of applicability, conclusions, recommendations, and related information for implementation of this remediation technology at the SRS Sanitary Landfill

The cell envelope subtilisin-like proteinase is a virulence determinant for Streptococcus suis

Directory of Open Access Journals (Sweden)

Gottschalk Marcelo

2010-02-01

Full Text Available Abstract Background Streptococcus suis is a major swine pathogen and zoonotic agent that mainly causes septicemia, meningitis, and endocarditis. It has recently been suggested that proteinases produced by S. suis (serotype 2 are potential virulence determinants. In the present study, we screened a S. suis mutant library created by the insertion of Tn917 transposon in order to isolate a mutant deficient in a cell surface proteinase. We characterized the gene and assessed the proteinase for its potential as a virulence factor. Results Two mutants (G6G and M3G possessing a single Tn917 insertion were isolated. The affected gene coded for a protein (SSU0757 that shared a high degree of identity with Streptococccus thermophilus PrtS (95.9% and, to a lesser extent, with Streptococcus agalactiae CspA (49.5%, which are cell surface serine proteinases. The SSU0757 protein had a calculated molecular mass of 169.6 kDa and contained the catalytic triad characteristic of subtilisin family proteinases: motif I (Asp200, motif II (His239, and motif III (Ser568. SSU0757 also had the Gram-positive cell wall anchoring motif (Leu-Pro-X-Thr-Gly at the carboxy-terminus, which was followed by a hydrophobic domain. All the S. suis isolates tested, which belonged to different serotypes, possessed the gene encoding the SSU0757 protein. The two mutants devoid of subtilisin-like proteinase activity had longer generation times and were more susceptible to killing by whole blood than the wild-type parent strain P1/7. The virulence of the G6G and M3G mutants was compared to the wild-type strain in the CD1 mouse model. Significant differences in mortality rates were noted between the P1/7 group and the M3G and G6G groups (p Conclusion In summary, we identified a gene coding for a cell surface subtilisin-like serine proteinase that is widely distributed in S. suis. Evidences were brought for the involvement of this proteinase in S. suis virulence.

Antimicrobial susceptibility of Streptococcus suis isolated from clinically healthy swine in Brazil.

Science.gov (United States)

Soares, Taíssa Cook Siqueira; Paes, Antonio Carlos; Megid, Jane; Ribolla, Paulo Eduardo Martins; Paduan, Karina dos Santos; Gottschalk, Marcelo

2014-04-01

Streptococcus suis is an important pathogen in the swine industry. This study is the first to report on the antimicrobial susceptibility of S. suis isolated from clinically healthy pigs in Brazil; the fourth major pork producer in the world. The antimicrobial susceptibility of 260 strains was determined by disc diffusion method. Strains were commonly susceptible to ceftiofur, cephalexin, chloramphenicol, and florfenicol, with more than 80% of the strains being susceptible to these antimicrobials. A high frequency of resistance to some of the antimicrobial agents was demonstrated, with resistance being most common to sulfa-trimethoprim (100%), tetracycline (97.69%), clindamycin (84.61%), norfloxacin (76.92%), and ciprofloxacin (61.15%). A high percentage of multidrug resistant strains (99.61%) were also found. The results of this study indicate that ceftiofur, cephalexin, and florfenicol are the antimicrobials of choice for empirical control of the infections caused by S. suis.

First district sanitary doctor of Katerynoslav

OpenAIRE

M.P. Chaban; Z.I. Shevtsova; V.V. Gaponov

2017-01-01

The life and professional activity of Vasyl Tymofiiovych Skrylnikov — a famous doctor-hygienist, scientist, and public figure have been represented. V.T. Skrylnikov contributed to the development of sanitary-prophylaxis direction in zemstvo medicine in Katerynoslav province in the second half of the 19th century; he was the first district sanitary doctor in Katerynoslav. The scientist actively studied medical features of natural agents, namely Tymofiivska clay. He successfully worked at Slovi...

Symptoms after ingestion of pig whipworm Trichuris suis eggs in a randomized placebo-controlled double-blind clinical trial

DEFF Research Database (Denmark)

Bager, Peter; Kapel, Christian Moliin Outzen; Roepstorff, Allan Knud

2011-01-01

by a fluoroenzymeimmunoassay (Phadia ApS). During 163 days complete follow-up, subjects ingesting T. suis eggs (N = 49) had a three to 19-fold higher rate of events (median duration, 2 days) with gastrointestinal reactions (moderate to severe flatulence, diarrhea, and upper abdominal pain) compared with placebo subjects (N......Symptoms after human infection with the helminth Trichuris suis have not previously been described. Exposure to helminths has been suggested as immune therapy against allergy and autoimmune diseases. We randomized adults with allergic rhinitis to ingest a dose of 2500 T. suis eggs or placebo every......-hoc analyses of gastrointestinal reactions. Adverse events and severity (mild, moderate, severe) were recorded daily by subjects, classified by organ using MedDRA 10.0, and event rates compared between subjects on T. suis treatment vs. subjects on placebo. T. suis-specific serum IgG antibodies were measured...

[Prophylactic requirements for sanitary and epidemiological surveillance in dentistry].

Science.gov (United States)

Kaplan, B M; Maksimenko, L V; Fedotova, N N; Gololobova, T V; Konovalov, O E

2009-01-01

The paper outlines the requirements for sanitary-and-epidemiological surveillance to prevent dental diseases. The investigations pose tasks to medical prevention centers to solve the problems in tooth prophylaxis, such as organizational-and-methodological, sanitary-and-educational, health-improving, and others. The sanitary-and-hygienic requirements for therapeutic-and-prophylactic dental facilities are defined. A procedure for keeping a management protocol for the prevention of tooth diseases is described.

10 CFR 20.2003 - Disposal by release into sanitary sewerage.

Science.gov (United States)

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Disposal by release into sanitary sewerage. 20.2003... Disposal § 20.2003 Disposal by release into sanitary sewerage. (a) A licensee may discharge licensed material into sanitary sewerage if each of the following conditions is satisfied: (1) The material is...

Development, Hatching, and Intestinal Establishment of Trichuris suis, - in vivo and in vitro Studies

DEFF Research Database (Denmark)

Vejzagic, Nermina

Trichuris suis, the pig whipworm is a nematode parasite located in the large intestine of pigs. Embryonated eggs of T. suis (T. suis ova = TSO) constitute the active pharmaceutical ingredient (API) in a new medicinal product, which is currently tested in human clinical trials as a potential treat...

Effect of Different Adjuvants on Protection and Side-Effects Induced by Helicobacter suis Whole-Cell Lysate Vaccination.

Directory of Open Access Journals (Sweden)

Iris Bosschem

Full Text Available Helicobacter suis (H. suis is a widespread porcine gastric pathogen, which is also of zoonotic importance. The first goal of this study was to investigate the efficacy of several vaccine adjuvants (CpG-DNA, Curdlan, Freund's Complete and Incomplete, Cholera toxin, administered either subcutaneously or intranasally along with H. suis whole-cell lysate, to protect against subsequent H. suis challenge in a BALB/c infection model. Subcutaneous immunization with Freund's complete (FC/lysate and intranasal immunization with Cholera toxin (CT/lysate were shown to be the best options for vaccination against H. suis, as determined by the amount of colonizing H. suis bacteria in the stomach, although adverse effects such as post-immunization gastritis/pseudo-pyloric metaplasia and increased mortality were observed, respectively. Therefore, we decided to test alternative strategies, including sublingual vaccine administration, to reduce the unwanted side-effects. A CCR4 antagonist that transiently inhibits the migration of regulatory T cells was also included as a new adjuvant in this second study. Results confirmed that immunization with CT (intranasally or sublingually is among the most effective vaccination protocols, but increased mortality was still observed. In the groups immunized subcutaneously with FC/lysate and CCR4 antagonist/lysate, a significant protection was observed. Compared to the FC/lysate immunized group, gastric pseudo-pyloric metaplasia was less severe or even absent in the CCR4 antagonist/lysate immunized group. In general, an inverse correlation was observed between IFN-γ, IL-4, IL-17, KC, MIP-2 and LIX mRNA expression and H. suis colonization density, whereas lower IL-10 expression levels were observed in partially protected animals.

Transcriptomic Analysis Reveals Selective Metabolic Adaptation of Streptococcus suis to Porcine Blood and Cerebrospinal Fluid

Directory of Open Access Journals (Sweden)

Anna Koczula

2017-02-01

Full Text Available Streptococcus suis is a zoonotic pathogen that can cause severe pathologies such as septicemia and meningitis in its natural porcine host as well as in humans. Establishment of disease requires not only virulence of the infecting strain but also an appropriate metabolic activity of the pathogen in its host environment. However, it is yet largely unknown how the streptococcal metabolism adapts to the different host niches encountered during infection. Our previous isotopologue profiling studies on S. suis grown in porcine blood and cerebrospinal fluid (CSF revealed conserved activities of central carbon metabolism in both body fluids. On the other hand, they suggested differences in the de novo amino acid biosynthesis. This prompted us to further dissect S. suis adaptation to porcine blood and CSF by RNA deep sequencing (RNA-seq. In blood, the majority of differentially expressed genes were associated with transport of alternative carbohydrate sources and the carbohydrate metabolism (pentose phosphate pathway, glycogen metabolism. In CSF, predominantly genes involved in the biosynthesis of branched-chain and aromatic amino acids were differentially expressed. Especially, isoleucine biosynthesis seems to be of major importance for S. suis in CSF because several related biosynthetic genes were more highly expressed. In conclusion, our data revealed niche-specific metabolic gene activity which emphasizes a selective adaptation of S. suis to host environments.

Prevalence of multiple drug resistant Streptococcus suis in and around Guwahati, India

Directory of Open Access Journals (Sweden)

Mrinalee Devi

2017-05-01

Full Text Available Aim: This study was conducted to determine the prevalence and antimicrobial susceptibility of Streptococcus suis and their resistance patterns isolated from both clinically healthy carriers and diseased pigs in and around Guwahati, Assam, India. Materials and Methods: A total of 497 samples were collected during October, 2012, to April, 2014, from clinically healthy (n=67 and diseased (n=230 pigs of varying age and either sex maintained under organized and unorganized farming systems. Samples were processed for isolation and identification of S. suis by biochemical characterization and polymerase chain reaction targeting the housekeeping gene glutamate dehydrogenase. In vitro antimicrobial susceptibility of the recovered isolates against nine antibiotic groups comprising 17 antimicrobial agents was studied by standard method. Results: Of the 497 samples examined, 7 (1.41% isolates were confirmed to be S. suis of which 5 (1.87% and 2 (0.87% were derived from clinically healthy and diseased pigs, respectively. All the isolates were susceptible to gentamicin, amikacin, and erythromycin (100% followed by the penicillin group and enrofloxacin (85.71%, ceftriaxone, doxycycline HCL, ofloxacin and chloramphenicol (71.43%, to kanamycin, clindamycin and co-trimoxazole (42.85%. The isolates showed least susceptibility to cefalexin, tetracycline and streptomycin (28.57%. All the five S. suis isolates from clinically healthy pigs were susceptible to penicillin G, amoxyclav, doxycycline HCl, gentamicin, amikacin and erythromycin, 80.00% isolates susceptible to ampicillin, enrofloxacin and ofloxacin, 60.00% to ceftriaxone, kanamycin and chloramphenicol, 40% to cefalexin, tetracycline, clindamycin and co-trimoxazole, respectively. Only 20.00% isolates were susceptible to streptomycin. Both the isolates recovered from diseased pigs were susceptible to ampicillin, ceftriaxone, gentamicin, amikacin, enrofloxacin, erythromycin, and clindamycin. On the other hand

30 CFR 71.501 - Sanitary toilet facilities; maintenance.

Science.gov (United States)

2010-07-01

... Section 71.501 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY HEALTH STANDARDS-SURFACE COAL MINES AND SURFACE WORK AREAS OF UNDERGROUND COAL MINES Sanitary Toilet Facilities at Surface Worksites of Surface Coal Mines § 71.501 Sanitary...

Virulence genes and genetic diversity of Streptococcus suis serotype 2 isolates from Thailand.

Science.gov (United States)

Maneerat, K; Yongkiettrakul, S; Kramomtong, I; Tongtawe, P; Tapchaisri, P; Luangsuk, P; Chaicumpa, W; Gottschalk, M; Srimanote, P

2013-11-01

Isolates of Streptococcus suis from different Western countries as well as those from China and Vietnam have been previously well characterized. So far, the genetic characteristics and relationship between S. suis strains isolated from both humans and pigs in Thailand are unknown. In this study, a total of 245 S. suis isolates were collected from both human cases (epidemic and sporadic) and pigs (diseased and asymptomatic) in Thailand. Bacterial strains were identified by biochemical tests and PCR targeting both, the 16S rRNA and gdh genes. Thirty-six isolates were identified as serotype 2 based on serotyping and the cps2-PCR. These isolates were tested for the presence of six virulence-associated genes: an arginine deiminase (arcA), a 38-kDa protein and protective antigen (bay046), an extracellular factor (epf), an hyaluronidase (hyl), a muramidase-released protein (mrp) and a suilysin (sly). In addition, the genetic diversities of these isolates were studied by RAPD PCR and multilocus sequence typing (MLST) analysis. Four virulence-associated gene patterns (VAGP 1 to 4) were obtained, and the majority of isolates (32/36) carried all genes tested (VAGP1). Each of the three OPB primers used provided 4 patterns designated RAPD-A to RAPD-D. Furthermore, MLST analysis could also distinguish the 36 isolates into four sequence types (STs): ST1 (n = 32), ST104 (n = 2), ST233 (n = 1) and a newly identified ST, ST336 (n = 1). Dendrogram constructions based on RAPD patterns indicated that S. suis serotype 2 isolates from Thailand could be divided into four groups and that the characteristics of the individual groups were in complete agreement with the virulence gene profiles and STs. The majority (32/36) of isolates recovered from diseased pigs, slaughterhouse pigs or human patients could be classified into a single group (VAGP1, RAPD-A and ST1). This genetic information strongly suggests the transmission of S. suis isolates from pigs to humans in Thailand. Our findings are

[To the 90th anniversary of State Sanitary Epidemiologic Service Formation in Russian Federation].

Science.gov (United States)

Beliaev, E N; Podunova, L G

2012-01-01

State Educational Institution of Higher Professional Training of the Ministry of Health Care and Social Development Foundation of Russian Sanitary Epidemiologic Service dates back to September, 15, 1922, when a Decree on Sanitary Institutions of the Republic was signed. Afterwards the institutions of Sanitary Epidemiologic Service were improved: foundation of State Sanitary Inspectorate (1933-1951), approval of new concept of the Service and intensification of State Sanitary Inspectorate (1951-1991), foundation of State Sanitary Epidemiologic Service, centralization of management and financing of the Service, formation of Sanitary Epidemiologic Legislation (1991-2004), further restructurization of Sanitary Epidemiologic Service in accordance with administrative changes in Russia (2004 until now).

Lysogenic Streptococcus suis isolate SS2-4 containing prophage SMP showed increased mortality in zebra fish compared to the wild-type isolate.

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Fang Tang

Full Text Available Streptococcus suis (S. suis infection is considered to be a major problem in the swine industry worldwide. Based on the capsular type, 33 serotypes of S. suis have been described, with serotype 2 (SS2 being the most frequently isolated from diseased piglets. Little is known, however, about the pathogenesis and virulence factors of S. suis. Research on bacteriophages highlights a new area in S. suis research. A S. suis serotype 2 bacteriophage, designated SMP, has been previously isolated in our laboratory. Here, we selected a lysogenic isolate in which the SMP phage was integrated into the chromosome of strain SS2-4. Compared to the wild-type isolate, the lysogenic strain showed increased mortality in zebra fish. Moreover the sensitivity of the lysogenic strain to lysozyme was seven times higher than that of the wild-type.

Establishment of a Cre recombinase based mutagenesis protocol for markerless gene deletion in Streptococcus suis.

Science.gov (United States)

Koczula, A; Willenborg, J; Bertram, R; Takamatsu, D; Valentin-Weigand, P; Goethe, R

2014-12-01

The lack of knowledge about pathogenicity mechanisms of Streptococcus (S.) suis is, at least partially, attributed to limited methods for its genetic manipulation. Here, we established a Cre-lox based recombination system for markerless gene deletions in S. suis serotype 2 with high selective pressure and without undesired side effects. Copyright © 2014 Elsevier B.V. All rights reserved.

Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma

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Sokoli Albina

2013-02-01

Full Text Available Abstract Hemotrophic mycoplasmas (HM are highly specialized red blood cell parasites that cause infectious anemia in a variety of mammals, including humans. To date, no in vitro cultivation systems for HM have been available, resulting in relatively little information about the pathogenesis of HM infection. In pigs, Mycoplasma suis-induced infectious anemia is associated with hemorrhagic diathesis, and coagulation dysfunction. However, intravasal coagulation and subsequent consumption coagulopathy can only partly explain the sequence of events leading to hemorrhagic diathesis manifesting as cyanosis, petechial bleeding, and ecchymosis, and to disseminated coagulation. The involvement of endothelial activation and damage in M. suis-associated pathogenesis was investigated using light and electron microscopy, immunohistochemistry, and cell sorting. M. suis interacted directly with endothelial cells in vitro and in vivo. Endothelial activation, widespread endothelial damage, and adherence of red blood cells to the endothelium were evident in M. suis-infected pigs. These alterations of the endothelium were accompanied by hemorrhage, intravascular coagulation, vascular occlusion, and massive morphological changes within the parenchyma. M. suis biofilm-like microcolonies formed on the surface of endothelial cells, and may represent a putative persistence mechanism of M. suis. In vitro analysis demonstrated that M. suis interacted with the endothelial cytoskeletal protein actin, and induced actin condensation and activation of endothelial cells, as determined by the up-regulation of ICAM, PECAM, E-selectin, and P-selectin. These findings demonstrate an additional cell tropism of HM for endothelial cells and suggest that M. suis interferes with the protective function of the endothelium, resulting in hemorrhagic diathesis.

Brucella suis vaccine strain 2 induces endoplasmic reticulum stress that affects intracellular replication in goat trophoblast cells in vitro

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Xiangguo eWang

2016-02-01

Full Text Available Brucella has been reported to impair placental trophoblasts, a cellular target where Brucella efficiently replicates in association with the endoplasmic reticulum (ER, and ultimately trigger abortion in pregnant animals. However, the precise effects of Brucella on trophoblast cells remain unclear. Here, we describe the infection and replication of Brucella suis vaccine strain 2 (B.suis.S2 in goat trophoblast cells (GTCs and the cellular and molecular responses induced in vitro. Our studies demonstrated that B.suis.S2 was able to infect and proliferate to high titers, hamper the proliferation of GTCs and induce apoptosis due to ER stress. Tunicamycin (Tm, a pharmacological chaperone that strongly mounts ER stress-induced apoptosis, inhibited B.suis.S2 replication in GTCs. In addition, 4 phenyl butyric acid (4-PBA, a pharmacological chaperone that alleviates ER stress-induced apoptosis, significantly enhanced B.suis.S2 replication in GTCs. The Unfolded Protein Response (UPR chaperone molecule GRP78 also promoted B.suis.S2 proliferation in GTCs by inhibiting ER stress-induced apoptosis. We also discovered that the IRE1 pathway, but not the PERK or ATF6 pathway, was activated in the process. However, decreasing the expression of phosphoIRE1α and IRE1α proteins with Irestatin 9389 (IRE1 antagonist in GTCs did not affect the proliferation of B.suis.S2. Although GTC implantation was not affected upon B.suis.S2 infection, progesterone secretion was suppressed, and prolactin and estrogen secretion increased; these effects were accompanied by changes in the expression of genes encoding key steroidogenic enzymes. This study systematically explored the mechanisms of abortion in Brucella infection from the viewpoint of pathogen invasion, ER stress and reproductive endocrinology. Our findings may provide new insight for understanding the mechanisms involved in goat abortions caused by Brucella infection.

First district sanitary doctor of Katerynoslav

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M.P. Chaban

2017-02-01

Full Text Available The life and professional activity of Vasyl Tymofiiovych Skrylnikov — a famous doctor-hygienist, scientist, and public figure have been represented. V.T. Skrylnikov contributed to the development of sanitary-prophylaxis direction in zemstvo medicine in Katerynoslav province in the second half of the 19th century; he was the first district sanitary doctor in Katerynoslav. The scientist actively studied medical features of natural agents, namely Tymofiivska clay. He successfully worked at Sloviansk resort, was the editor of a local newspaper. V.T. Skrylnikov is an author of many works on balneotherapy.

Purification and Characterization of Suicin 65, a Novel Class I Type B Lantibiotic Produced by Streptococcus suis.

Science.gov (United States)

Vaillancourt, Katy; LeBel, Geneviève; Frenette, Michel; Fittipaldi, Nahuel; Gottschalk, Marcelo; Grenier, Daniel

2015-01-01

Bacteriocins are antimicrobial peptides of bacterial origin that are considered as a promising alternative to the use of conventional antibiotics. Recently, our laboratory reported the purification and characterization of two lantibiotics, suicin 90-1330 and suicin 3908, produced by the swine pathogen and zoonotic agent Streptococcus suis (serotype 2). In this study, a novel bacteriocin produced by S. suis has been identified and characterized. The producing strain S. suis 65 (serotype 2) was found to belong to the sequence type 28, that includes strains known to be weakly or avirulent in a mouse model. The bacteriocin, whose production was only possible following growth on solid culture medium, was purified to homogeneity by cationic exchange and reversed-phase high-pressure liquid chromatography. The bacteriocin, named suicin 65, was heat, pH and protease resistant. Suicin 65 was active against all S. suis isolates tested, including antibiotic resistant strains. Amino acid sequencing of the purified bacteriocin by Edman degradation revealed the presence of modified amino acids suggesting a lantibiotic. Using the partial sequence obtained, a blast was performed against published genomes of S. suis and allowed to identify a putative lantibiotic locus in the genome of S. suis 89-1591. From this genome, primers were designed and the gene cluster involved in the production of suicin 65 by S. suis 65 was amplified by PCR. Sequence analysis revealed the presence of ten open reading frames, including a duplicate of the structural gene. The structural genes (sssA and sssA') of suicin 65 encodes a 25-amino acid residue leader peptide and a 26-amino acid residue mature peptide yielding an active bacteriocin with a deducted molecular mass of 3,005 Da. Mature suicin 65 showed a high degree of identity with class I type B lantibiotics (globular structure) produced by Streptococcus pyogenes (streptococcin FF22; 84.6%), Streptococcus macedonicus (macedocin ACA-DC 198; 84

Purification and Characterization of Suicin 65, a Novel Class I Type B Lantibiotic Produced by Streptococcus suis.

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Katy Vaillancourt

Full Text Available Bacteriocins are antimicrobial peptides of bacterial origin that are considered as a promising alternative to the use of conventional antibiotics. Recently, our laboratory reported the purification and characterization of two lantibiotics, suicin 90-1330 and suicin 3908, produced by the swine pathogen and zoonotic agent Streptococcus suis (serotype 2. In this study, a novel bacteriocin produced by S. suis has been identified and characterized. The producing strain S. suis 65 (serotype 2 was found to belong to the sequence type 28, that includes strains known to be weakly or avirulent in a mouse model. The bacteriocin, whose production was only possible following growth on solid culture medium, was purified to homogeneity by cationic exchange and reversed-phase high-pressure liquid chromatography. The bacteriocin, named suicin 65, was heat, pH and protease resistant. Suicin 65 was active against all S. suis isolates tested, including antibiotic resistant strains. Amino acid sequencing of the purified bacteriocin by Edman degradation revealed the presence of modified amino acids suggesting a lantibiotic. Using the partial sequence obtained, a blast was performed against published genomes of S. suis and allowed to identify a putative lantibiotic locus in the genome of S. suis 89-1591. From this genome, primers were designed and the gene cluster involved in the production of suicin 65 by S. suis 65 was amplified by PCR. Sequence analysis revealed the presence of ten open reading frames, including a duplicate of the structural gene. The structural genes (sssA and sssA' of suicin 65 encodes a 25-amino acid residue leader peptide and a 26-amino acid residue mature peptide yielding an active bacteriocin with a deducted molecular mass of 3,005 Da. Mature suicin 65 showed a high degree of identity with class I type B lantibiotics (globular structure produced by Streptococcus pyogenes (streptococcin FF22; 84.6%, Streptococcus macedonicus (macedocin ACA

Seroepidemiologic survey for Chlamydia suis in wild boar (Sus scrofa) populations in Italy.

Science.gov (United States)

Di Francesco, Antonietta; Donati, Manuela; Morandi, Federico; Renzi, Maria; Masia, Marco Antonio; Ostanello, Fabio; Salvatore, Daniela; Cevenini, Roberto; Baldelli, Raffaella

2011-07-01

We used serology to estimate the prevalence of exposure to chlamydiae in Italian populations of wild boars (Sus scrofa). Sera from 173 hunter-killed wild boars harvested during the 2006-2009 hunting seasons in three Italian regions were tested for antibodies to Chlamydia suis, Chlamydophila pecorum, Chlamydophila abortus, and Chlamydophila psittaci by the microimmunofluorescence test. Antibody titers to chlamydiae ≥ 1:32 were detected in 110 of the 173 samples tested (63.6%). Specific reactivity could be assessed only in 44 sera with antibody titers to C. suis that were two- to threefold higher than antibody titers against the other chlamydial species; the other 66 sera had similar reactivity against all the chlamydia species tested. Antibody to C. suis was detected in sera from wild boar populations with rare or no known contact with domestic pigs. These results suggest that the wild boar could be a chlamydia reservoir and may acquire chlamydiae independent of contacts with the domestic pig.

Problems of sanitary-hygienic arrangements in case of emergency situations

International Nuclear Information System (INIS)

Avetisov, G.M.; Borisov, B.K.; Volkov, N.N.; Grachev, M.I.; Korostin, A.S.; Prostakishin, G.P.

1995-01-01

The planning and carrying out sanitary-hygienic arrangements in emergency situation are based on scientific-research, organizing-methodical and practical trends of activities including: the necessity of taking into consideration the risk of sanitary-hygienic complications as a result of emergency situation; elaboration of criteria and classification of hazards potential danger of industrial plants and territories; the establishment of system of providing sanitary-hygienic measures in emergency situation; working out prophylactic measures for prevention, localization and liquidation of sanitary-hygienic after-effects of emergency situation; development of the system of control and express-analysis of hazardous factors; elaboration of rating-methodical base and arrangements aimed at protection of health and life of people. 5 refs

Enzymatic, immunological and phylogenetic characterization of Brucella suis urease

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Sriranganathan Nammalwar

2008-07-01

Full Text Available Abstract Background The sequenced genomes of the Brucella spp. have two urease operons, ure-1 and ure-2, but there is evidence that only one is responsible for encoding an active urease. The present work describes the purification and the enzymatic and phylogenomic characterization of urease from Brucella suis strain 1330. Additionally, the urease reactivity of sera from patients diagnosed with brucellosis was examined. Results Urease encoded by the ure-1 operon of Brucella suis strain 1330 was purified to homogeneity using ion exchange and hydrophobic interaction chromatographies. The urease was purified 51-fold with a recovery of 12% of the enzyme activity and 0.24% of the total protein. The enzyme had an isoelectric point of 5, and showed optimal activity at pH 7.0 and 28–35°C. The purified enzyme exhibited a Michaelis-Menten saturation kinetics with a Km of 5.60 ± 0.69 mM. Hydroxyurea and thiourea are competitive inhibitors of the enzyme with Ki of 1.04 ± 0.31 mM and 26.12 ± 2.30 mM, respectively. Acetohydroxamic acid also inhibits the enzyme in a competitive way. The molecular weight estimated for the native enzyme was between 130–135 kDa by gel filtration chromatography and 157 ± 7 kDa using 5–10% polyacrylamide gradient non-denaturing gel. Only three subunits in SDS-PAGE were identified: two small subunits of 14,000 Da and 15,500 Da, and a major subunit of 66,000 Da. The amino terminal sequence of the purified large subunit corresponded to the predicted amino acid sequence encoded by ureC1. The UreC1 subunit was recognized by sera from patients with acute and chronic brucellosis. By phylogenetic and cluster structure analyses, ureC1 was related to the ureC typically present in the Rhizobiales; in contrast, the ureC2 encoded in the ure-2 operon is more related to distant species. Conclusion We have for the first time purified and characterized an active urease from B. suis. The enzyme was characterized at the kinetic

Associations of Streptococcus suis serotype 2 ribotype profiles with clinical disease and antimicrobial resistance

DEFF Research Database (Denmark)

Rasmussen, S. R.; Aarestrup, Frank Møller; Jensen, N. E.

1999-01-01

A total of 122 Streptococcus suis serotype 2 strains were characterized thoroughly by comparing clinical and pathological observations, ribotype profiles, and antimicrobial resistance. Twenty-one different ribotype profiles were found and compared by cluster analysis, resulting in the identificat......A total of 122 Streptococcus suis serotype 2 strains were characterized thoroughly by comparing clinical and pathological observations, ribotype profiles, and antimicrobial resistance. Twenty-one different ribotype profiles were found and compared by cluster analysis, resulting...

Streptococcus suis meningoencephalitis with seizure from raw pork ingestion: a case report.

Science.gov (United States)

Wongjittraporn, Suwarat; Teerasukjinda, Ornusa; Yee, Melvin; Chung, Heath H

2014-09-01

Streptococcus suis meningoencephalitis is a rare but increasingly important condition. Good history taking will give clues to the diagnosis. This is the fourth case report in the United States. A 52-year-old Filipino man who recently returned from a trip to the Philippines was admitted with classic symptoms of bacterial meningitis. His cerebrospinal fluid culture grew Streptococcus suis. His clinical course was complicated by seizures, hearing loss, and permanent tinnitus. Clinicians should be aware of this emerging disease especially in patients with recent travel history to endemic areas. Early recognition and appropriate management could potentially prevent complications.

Sanitary effects of fossil fuels; Effets sanitaires des combustibles fossiles

Energy Technology Data Exchange (ETDEWEB)

Nifenecker, H. [Centre National de la Recherche Scientifique (IN2P3/CNRS), 38 - Grenoble (France)

2006-07-01

In this compilation are studied the sanitary effects of fossil fuels, behavioral and environmental sanitary risks. The risks in connection with the production, the transport and the distribution(casting) are also approached for the oil(petroleum), the gas and the coal. Accidents in the home are evoked. The risks due to the atmospheric pollution are seen through the components of the atmospheric pollution as well as the sanitary effects of this pollution. (N.C.)

A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador

DEFF Research Database (Denmark)

Meekums, Hayley; Hawash, Mohamed B F; Sparks, Alexandra M

2015-01-01

BACKGROUND: Since the nematodes Trichuris trichiura and T. suis are morphologically indistinguishable, genetic analysis is required to assess epidemiological cross-over between people and pigs. This study aimed to clarify the transmission biology of trichuriasis in Ecuador. FINDINGS: Adult...... Trichuris worms were collected during a parasitological survey of 132 people and 46 pigs in Esmeraldas Province, Ecuador. Morphometric analysis of 49 pig worms and 64 human worms revealed significant variation. In discriminant analysis morphometric characteristics correctly classified male worms according...... to genetically analyse Trichuris parasites. Although T. trichiura does not appear to be zoonotic in Ecuador, there is evidence of genetic exchange between T. trichiura and T. suis warranting more detailed genetic sampling....

Stimulating the development of national Streptococcus suis guidelines in Viet Nam through a strategic research partnership.

Science.gov (United States)

Horby, Peter; Wertheim, Heiman; Ha, Nguyen Hong; Trung, Nguyen Vu; Trinh, Dao Tuyet; Taylor, Walter; Ha, Nguyen Minh; Lien, Trinh Thi Minh; Farrar, Jeremy; Van Kinh, Nguyen

2010-06-01

Streptococcus suis is a common cause of adult bacterial meningitis in Viet Nam, and possibly other parts of Asia, yet this disabling infection has been largely neglected. Prevention, diagnosis and treatment are relatively straightforward and affordable but, in early 2007, no national diagnostic, case management or prevention guidelines existed in Viet Nam. Enhanced detection of S. suis infections was established in 2007 as part of a collaborative research programme between the National Hospital for Tropical Diseases, a key national hospital with very close links to the Ministry of Health, and a research group affiliated with Oxford University based in Viet Nam. The results were reported directly to policy-makers at the Ministry of Health. Viet Nam is a low-income country with a health-care system that has seen considerable improvements and increased autonomy. However, parts of the system remain fairly centralized the Ministry of Health. Following the improved detection and reporting of S. suis cases, the Ministry of Health issued guidance to all hospitals in Viet Nam on the clinical and laboratory diagnosis, treatment and prevention of S. suis. A public health laboratory diagnostic service was established at the National Institute of Hygiene and Epidemiology and training courses were conducted for clinicians and microbiologists. Ministry of Health guidance on surveillance and control of communicable diseases was updated to include a section on S. suis. Research collaborations can efficiently inform and influence national responses if they are well positioned to reach policy-makers.

Antimicrobial Resistance Profile and Genotypic Characteristics of Streptococcus suis Capsular Type 2 Isolated from Clinical Carrier Sows and Diseased Pigs in China

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Chunping Zhang

2015-01-01

Full Text Available Streptococcus suis serotype 2 is an important zoonotic pathogen. Antimicrobial resistance phenotypes and genotypic characterizations of S. suis 2 from carrier sows and diseased pigs remain largely unknown. In this study, 96 swine S. suis type 2, 62 from healthy sows and 34 from diseased pigs, were analyzed. High frequency of tetracycline resistance was observed, followed by sulfonamides. The lowest resistance of S. suis 2 for β-lactams supports their use as the primary antibiotics to treat the infection of serotype 2. In contrast, 35 of 37 S. suis 2 with MLSB phenotypes were isolated from healthy sows, mostly encoded by the ermB and/or the mefA genes. Significantly lower frequency of mrp+/epf+/sly+ was observed among serotype 2 from healthy sows compared to those from diseased pigs. Furthermore, isolates from diseased pigs showed more homogeneously genetic patterns, with most of them clustered in pulsotypes A and E. The data indicate the genetic complexity of S. suis 2 between herds and a close linkage among isolates from healthy sows and diseased pigs. Moreover, many factors, such as extensive use of tetracycline or diffusion of Tn916 with tetM, might have favored for the pathogenicity and widespread dissemination of S. suis serotype 2.

FlpS, the FNR-Like Protein of Streptococcus suis Is an Essential, Oxygen-Sensing Activator of the Arginine Deiminase System

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Jörg Willenborg

2016-07-01

Full Text Available Streptococcus (S. suis is a zoonotic pathogen causing septicemia and meningitis in pigs and humans. During infection S. suis must metabolically adapt to extremely diverse environments of the host. CcpA and the FNR family of bacterial transcriptional regulators are important for metabolic gene regulation in various bacteria. The role of CcpA in S. suis is well defined, but the function of the FNR-like protein of S. suis, FlpS, is yet unknown. Transcriptome analyses of wild-type S. suis and a flpS mutant strain suggested that FlpS is involved in the regulation of the central carbon, arginine degradation and nucleotide metabolism. However, isotopologue profiling revealed no substantial changes in the core carbon and amino acid de novo biosynthesis. FlpS was essential for the induction of the arcABC operon of the arginine degrading pathway under aerobic and anaerobic conditions. The arcABC-inducing activity of FlpS could be associated with the level of free oxygen in the culture medium. FlpS was necessary for arcABC-dependent intracellular bacterial survival but redundant in a mice infection model. Based on these results, we propose that the core function of S. suis FlpS is the oxygen-dependent activation of the arginine deiminase system.

Virulence-associated gene profiling of Streptococcus suis isolates by PCR

NARCIS (Netherlands)

Silva, L.M.G.; Baums, C.G.; Rehm, T.; Wisselink, H.J.; Goethe, R.; Valentin-Weigand, P.

2006-01-01

Definition of virulent Streptococcus suis strains is controversial. One successful approach for identification of virulent European strains is differentiation of capsular serotypes (or the corresponding cps types) and subsequent detection of virulence-associated factors, namely the extracellular

Symptoms after ingestion of pig whipworm Trichuris suis eggs in a randomized placebo-controlled double-blind clinical trial

DEFF Research Database (Denmark)

Bager, Peter; Kapel, Christian Moliin Outzen; Roepstorff, Allan Knud

2011-01-01

Symptoms after human infection with the helminth Trichuris suis have not previously been described. Exposure to helminths has been suggested as immune therapy against allergy and autoimmune diseases. We randomized adults with allergic rhinitis to ingest a dose of 2500 T. suis eggs or placebo every...... by a fluoroenzymeimmunoassay (Phadia ApS). During 163 days complete follow-up, subjects ingesting T. suis eggs (N = 49) had a three to 19-fold higher rate of events (median duration, 2 days) with gastrointestinal reactions (moderate to severe flatulence, diarrhea, and upper abdominal pain) compared with placebo subjects (N...... of episodes with onset before day 42 was ≤ 14 days in 80% of affected subjects. Age, gender, total IgE, and recent intestinal symptoms at baseline did not predict gastrointestinal side effects. In conclusion, during the first 2 months, repeated ingestions of 2500 T. suis eggs caused frequent gastrointestinal...

The whipworm (Trichuris suis) secretes prostaglandin E2 to suppress proinflammatory properties in human dendritic cells

DEFF Research Database (Denmark)

Laan, Lisa C; Williams, Andrew R; Stavenhagen, Kathrin

2017-01-01

Clinical trials have shown that administration of the nematode Trichuris suis can be beneficial in treating various immune disorders. To provide insight into the mechanisms by which this worm suppresses inflammatory responses, an active component was purified from T. suis soluble products (TsSPs)...

Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS caused by Streptococcus suis

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Chao eYang

2015-11-01

Full Text Available Streptococcus suis (S. suis, a major swine pathogen, is also a severe threat to human health. Infection with highly virulent strains of S. suis can cause human Streptococcal toxic-shock-like syndrome (STSLS, which is associated with high serum pro-inflammatory cytokine levels and a high mortality rate. Our previous study indicated that highly virulent S. suis infection could activate the TREM-1 signaling pathway, which promotes host clearance of S. suis during early infection. However, it remained to be elicited whether TREM-1 signaling could be a target against STSLS in the presence of antibiotic. In the present study, mice were infected with a highly virulent S. suis strain and then treated with rTREM-1 (the recombinant extracellular domain of TREM-1 to block TREM-1 signaling, antibiotics, both rTREM-1 and antibiotics, or PBS. The survival rates, clinical signs, serum IL-1β and TNF-α levels, and serum bacterial loads were evaluated. Treatment with rTREM-1 could aggravate the outcome of infection as described previously. Although the conventional treatment with antibiotics contributed to effective S. suis clearance, it did not improve survival significantly. In comparison, due to the reduction of the exaggerated pro-inflammatory response, treatment combined with rTREM-1 and antibiotics not only led to efficient bacterial clearance but also alleviated inflammation. In conclusion, TREM-1 signaling contributed to severe inflammatory response and benefited S. suis clearance. Therefore, blocking TREM-1 signaling could still be a target for the treatment of STSLS in the presence of antibiotics.

Hostos y el Positivismo sui Generis Latinoamericano

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Pablo Guadarrama González

2004-01-01

Full Text Available El positivismo fue asumido y cultivado en Latinoamérica de forma sui generis como una filosofía optimista llena de confianza en el hombre, en la capacidad creativa de su pensamiento, en la cultura, la educación, la ciencia, el progreso y el desarrollo industrial. Aliado al liberalismo y a la defensa de la democracia sus ideas resultaban muy avanzadas para los países latinoamericanos, recién liberados en su mayoría del colonialismo español y enfrascados entonces en profundas luchas entre las oligarquías retrogradas y la naciente burguesía nacional. Las ideas filosóficas y educativas de Hostos ponen de manifiesto la riqueza alcanzada por el pensamiento latinoamericano en el siglo XIX que aunque compartió algunas posiciones con el krausismo y el positivismo no se dejó arrastrar de forma unilateral por estas corrientes y supo aprovechar los elementos de valor contenidos especialmente en el positivismo que supo asumir de modo sui generis como la mayoría de los intelectuales latinoamericanos de esa época que se identificaron con él en correspondencia con una praxis educativa y política beneficiosa a los pueblos de América Latina.

Slaughterhouse pigs are a major reservoir of Streptococcus suis serotype 2 capable of causing human infection in southern Vietnam.

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Thi Hoa Ngo

2011-03-01

Full Text Available Streptococcus suis is a pathogen of major economic significance to the swine industry and is increasingly recognized as an emerging zoonotic agent in Asia. In Vietnam, S. suis is the leading cause of bacterial meningitis in adult humans. Zoonotic transmission is most frequently associated with serotype 2 strains and occupational exposure to pigs or consumption of infected pork. To gain insight into the role of pigs for human consumption as a reservoir for zoonotic infection in southern Vietnam, we determined the prevalence and diversity of S. suis carriage in healthy slaughterhouse pigs. Nasopharyngeal tonsils were sampled from pigs at slaughterhouses serving six provinces in southern Vietnam and Ho Chi Minh City area from September 2006 to November 2007. Samples were screened by bacterial culture. Isolates of S. suis were serotyped and characterized by multi locus sequence typing (MLST and pulse field gel electrophoresis (PFGE. Antibiotic susceptibility profiles and associated genetic resistance determinants, and the presence of putative virulence factors were determined. 41% (222/542 of pigs carried S. suis of one or multiple serotypes. 8% (45/542 carried S. suis serotype 2 which was the most common serotype found (45/317 strains, 14%. 80% of serotype 2 strains belonged to the MLST clonal complex 1,which was previously associated with meningitis cases in Vietnam and outbreaks of severe disease in China in 1998 and 2005. These strains clustered with representative strains isolated from patients with meningitis in PFGE analysis, and showed similar antimicrobial resistance and virulence factor profiles. Slaughterhouse pigs are a major reservoir of S. suis serotype 2 capable of causing human infection in southern Vietnam. Strict hygiene at processing facilities, and health education programs addressing food safety and proper handling of pork should be encouraged.

Research and development of intelligent controller for high-grade sanitary ware

Science.gov (United States)

Bao, Kongjun; Shen, Qingping

2013-03-01

With the social and economic development and people's living standards improve, more and more emphasis on modern society, people improve the quality of family life, the use of intelligent controller applications in high-grade sanitary ware physiotherapy students. Analysis of high-grade sanitary ware physiotherapy common functions pointed out in the production and use of the possible risks, proposed implementation of the system hardware and matching, given the system software implementation process. High-grade sanitary ware physiotherapy intelligent controller not only to achieve elegant and beautiful, simple, physical therapy, water power, deodorant, multi-function, intelligent control, to meet the consumers, the high-end sanitary ware market, strong demand, Accelerate the enterprise product Upgrade and improve the competitiveness of enterprises.

Detection of Streptococcus suis by in situ hybridization, indirect immunofluorescence, and peroxidase-antiperoxidase assays in formalin-fixed, paraffin-embedded tissue sections from pigs

DEFF Research Database (Denmark)

Boye, Mette; Feenstra, Anne Avlund; Tegtmeier, Conny

2000-01-01

and the immunohistochemical methods were used for detection of S. suis in formalin-fixed, paraffin-embedded tissue sections of brain, endocardium, and lung from pigs infected with S. suis. The methods developed were able to detect single cells of S. suis in situ in the respective samples, whereas no signal was observed from...

Binding of Human Fibrinogen to MRP Enhances Streptococcus suis Survival in Host Blood in a αXβ2 Integrin-dependent Manner.

Science.gov (United States)

Pian, Yaya; Li, Xueqin; Zheng, Yuling; Wu, Xiaohong; Yuan, Yuan; Jiang, Yongqiang

2016-05-27

The Gram-positive bacterium Streptococcus suis serotype 2 (S. suis 2), an important zoonotic pathogen, induces strong systemic infections in humans; sepsis and meningitis are the most common clinical manifestations and are often accompanied by bacteremia. However, the mechanisms of S. suis 2 survival in human blood are not well understood. In our previous study, we identified muramidase-released protein (MRP), a novel human fibrinogen (hFg)-binding protein (FBP) in S. suis 2 that is an important epidemic infection marker with an unknown mechanism in pathogenesis. The present study demonstrates that the N-terminus of MRP (a.a. 283-721) binds to both the Aα and Bβ chains of the D fragment of hFg. Strikingly, the hFg-MRP interaction improved the survival of S. suis 2 in human blood and led to the aggregation and exhaustion of polymorphonuclear neutrophils (PMNs) via an αXβ2 integrin-dependent mechanism. Other Fg-binding proteins, such as M1 (GAS) and FOG (GGS), also induced PMNs aggregation; however, the mechanisms of these FBP-hFg complexes in the evasion of PMN-mediated innate immunity remain unclear. MRP is conserved across highly virulent strains in Europe and Asia, and these data shed new light on the function of MRP in S. suis pathogenesis.

PK-PD Integration Modeling and Cutoff Value of Florfenicol against Streptococcus suis in Pigs

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Zhixin Lei

2018-01-01

Full Text Available The aims of the present study were to establish optimal doses and provide an alternate COPD for florfenicol against Streptococcus suis based on pharmacokinetic-pharmacodynamic integration modeling. The recommended dose (30 mg/kg b.w. were administered in healthy pigs through intramuscular and intravenous routes for pharmacokinetic studies. The main pharmacokinetic parameters of Cmax, AUC0-24h, AUC, Ke, t1/2ke, MRT, Tmax, and Clb, were estimated as 4.44 μg/ml, 88.85 μg⋅h/ml, 158.56 μg⋅h/ml, 0.048 h-1, 14.46 h, 26.11 h, 4 h and 0.185 L/h⋅kg, respectively. The bioavailability of florfenicol was calculated to be 99.14% after I.M administration. A total of 124 Streptococcus suis from most cities of China were isolated to determine the minimum inhibitory concentration (MIC of florfenicol. The MIC50 and MIC90 were calculated as 1 and 2 μg/ml. A serotype 2 Streptococcus suis (WH-2, with MIC value similar to MIC90, was selected as a representative for an in vitro and ex vivo pharmacodynamics study. The MIC values of WH-2 in TSB and plasma were 2 μg/ml, and the MBC/MIC ratios were 2 in TSB and plasma. The MPC was detected to be 3.2 μg/ml. According to inhibitory sigmoid Emax model, plasma AUC0-24h/MIC values of florfenicol versus Streptococcus suis were 37.89, 44.02, and 46.42 h for the bactericidal, bacteriostatic, and elimination activity, respectively. Monte Carlo simulations the optimal doses for bactericidal, bacteriostatic, and elimination effects were calculated as 16.5, 19.17, and 20.14 mg/kg b.w. for 50% target attainment rates (TAR, and 21.55, 25.02, and 26.85 mg/kg b.w. for 90% TAR, respectively. The PK-PD cutoff value (COPD analyzed from MCS for florfenicol against Streptococcus suis was 1 μg/ml which could provide a sensitivity cutoff value. These results contributed an optimized alternative to clinical veterinary medicine and showed that the dose of 25.02 mg/kg florfenicol for 24 h could have a bactericidal action against

Streptococcus suis, an important pig pathogen and emerging zoonotic agent—an update on the worldwide distribution based on serotyping and sequence typing

Science.gov (United States)

Goyette-Desjardins, Guillaume; Auger, Jean-Philippe; Xu, Jianguo; Segura, Mariela; Gottschalk, Marcelo

2014-01-01

Streptococcus suis is an important pathogen causing economic problems in the pig industry. Moreover, it is a zoonotic agent causing severe infections to people in close contact with infected pigs or pork-derived products. Although considered sporadic in the past, human S. suis infections have been reported during the last 45 years, with two large outbreaks recorded in China. In fact, the number of reported human cases has significantly increased in recent years. In this review, we present the worldwide distribution of serotypes and sequence types (STs), as determined by multilocus sequence typing, for pigs (between 2002 and 2013) and humans (between 1968 and 2013). The methods employed for S. suis identification and typing, the current epidemiological knowledge regarding serotypes and STs and the zoonotic potential of S. suis are discussed. Increased awareness of S. suis in both human and veterinary diagnostic laboratories and further establishment of typing methods will contribute to our knowledge of this pathogen, especially in regions where complete and/or recent data is lacking. More research is required to understand differences in virulence that occur among S. suis strains and if these differences can be associated with specific serotypes or STs. PMID:26038745

Evolution and genome specialization of Brucella suis biovar 2 Iberian lineages.

Science.gov (United States)

Ferreira, Ana Cristina; Tenreiro, Rogério; de Sá, Maria Inácia Corrêa; Dias, Ricardo

2017-09-12

Swine brucellosis caused by B. suis biovar 2 is an emergent disease in domestic pigs in Europe. The emergence of this pathogen has been linked to the increase of extensive pig farms and the high density of infected wild boars (Sus scrofa). In Portugal and Spain, the majority of strains share specific molecular characteristics, which allowed establishing an Iberian clonal lineage. However, several strains isolated from wild boars in the North-East region of Spain are similar to strains isolated in different Central European countries. Comparative analysis of five newly fully sequenced B. suis biovar 2 strains belonging to the main circulating clones in Iberian Peninsula, with publicly available Brucella spp. genomes, revealed that strains from Iberian clonal lineage share 74% similarity with those reference genomes. Besides the 210 kb translocation event present in all biovar 2 strains, an inversion with 944 kb was presented in chromosome I of strains from the Iberian clone. At left and right crossover points, the inversion disrupted a TRAP dicarboxylate transporter, DctM subunit, and an integral membrane protein TerC. The gene dctM is well conserved in Brucella spp. except in strains from the Iberian clonal lineage. Intraspecies comparative analysis also exposed a number of biovar-, haplotype- and strain-specific insertion-deletion (INDELs) events and single nucleotide polymorphisms (SNPs) that could explain differences in virulence and host specificities. Most discriminative mutations were associated to membrane related molecules (29%) and enzymes involved in catabolism processes (20%). Molecular identification of both B. suis biovar 2 clonal lineages could be easily achieved using the target-PCR procedures established in this work for the evaluated INDELs. Whole-genome analyses supports that the B. suis biovar 2 Iberian clonal lineage evolved from the Central-European lineage and suggests that the genomic specialization of this pathogen in the Iberian Peninsula

Sanitary-epidemiological audit in russia and abroad: challenges and growth prospects (analytical review

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I.V. May

2016-12-01

Full Text Available The shaping the system of control and surveillance activities in the Russian Federation, which affects the bodies of the sanitary-epidemiological surveillance, requires the development of new forms of cooperation between authorities and legal entities, individual entrepreneurs and population. Such a form may be represented by the sanitary and epidemiological audit as an independent objective assessment of reputable third parties. The audit is intended to check the compliance with the mandatory requirements of the economic entities, performing economic or other activities. The sanitary-epidemiological audit may be associated with a system of certification for compliance with sanitary requirements and may assume the documented confirmation of the compliance issued by the authorized persons. The sanitary-epidemiological audit and the compliance certification to mandatory sanitary requirements can make an alternative to the planned supervision activities on facilities attributed to the category of low and moderate risk of harm to human health. The Russian sanitary legislation does not recognize the sanitary-epidemiological audit as a form of conformity assessment. The analysis of the international experience shows that it is necessary to consolidate the general rules and regulations of the sanitary-epidemiological audit at the legislative level and to develop a set of sublegislative documents in order to implement these norms. The crucial is a creation of the national system capable to regulate the registration and functioning of the organization having a right to conduct the audit activities in the field of hygienic safety. It is reasonable to develop the regulation on the list-register of auditors and to create a special training system for the auditors, who possess the methodology for health risk assessment. The key aspect of the successful introduction of the audit is a granting of presences to the economic entities having compliance

Planning solutions of sanitary facilities in modern residential buildings

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Orlov Evgeniy Vladimirovich

2015-01-01

Full Text Available In the article the short historical review on the design of sanitary rooms and their configurations is given. The main errors of the recent years, which led to the decrease in accommodation convenience because of the wrong approach from both the architect and engineers, are given. It is possible to use a small useful area for sanitary facilities, but it is connected with the lack of possibility of connecting washing and dishwashers. The author considers the options of engineering equipment placement in sanitary rooms taking into account the convenience of use, safety, and also resource-saving aspect. Various solutions on the organization of heating and ventilation are provided. The possible technical solutions allowing solving a flooding problem of the first floors in elite housing estates in case of accident are offered with the help of full waterproofing of sanitary rooms, and also the whole area of the apartment. The main attention was focused on the improvements of sanitary rooms for one-room and two-room apartments, which are the most demanded in the modern market of real estate. Layout solutions of the reduced bathrooms on the placement of the necessary equipment with choice justification are provided. The attention is paid to the layout solution for modern kitchens on order to increase their comfort by the use of special two-section sinks, and also a grinder of food waste in order to allow to lower the load of the systems of rubbish disposal of a building, by dumping the crushed garbage in an internal sewer network. Various options of evolutionary development of sanitary rooms for increasing the comfort degree are given. First of all, the development should happen in the direction of not only sanitation and hygiene, but also of the maintenance of the physical health of the people living in the building. It can be carried out by increase in a useful area of sanitary rooms, installation of exercise machines, medical bathtubs and a Jacuzzi

Ants avoid superinfections by performing risk-adjusted sanitary care.

Science.gov (United States)

Konrad, Matthias; Pull, Christopher D; Metzler, Sina; Seif, Katharina; Naderlinger, Elisabeth; Grasse, Anna V; Cremer, Sylvia

2018-03-13

Being cared for when sick is a benefit of sociality that can reduce disease and improve survival of group members. However, individuals providing care risk contracting infectious diseases themselves. If they contract a low pathogen dose, they may develop low-level infections that do not cause disease but still affect host immunity by either decreasing or increasing the host's vulnerability to subsequent infections. Caring for contagious individuals can thus significantly alter the future disease susceptibility of caregivers. Using ants and their fungal pathogens as a model system, we tested if the altered disease susceptibility of experienced caregivers, in turn, affects their expression of sanitary care behavior. We found that low-level infections contracted during sanitary care had protective or neutral effects on secondary exposure to the same (homologous) pathogen but consistently caused high mortality on superinfection with a different (heterologous) pathogen. In response to this risk, the ants selectively adjusted the expression of their sanitary care. Specifically, the ants performed less grooming and more antimicrobial disinfection when caring for nestmates contaminated with heterologous pathogens compared with homologous ones. By modulating the components of sanitary care in this way the ants acquired less infectious particles of the heterologous pathogens, resulting in reduced superinfection. The performance of risk-adjusted sanitary care reveals the remarkable capacity of ants to react to changes in their disease susceptibility, according to their own infection history and to flexibly adjust collective care to individual risk.

Expulsion of the swine whipworm, Trichuris suis 

DEFF Research Database (Denmark)

Kringel, Helene

demonstrating the potential use of its eggs as immunomodulators, treating patients suffering from inflammatory bowel disease and possibly other autoimmune diseases. The immune response induced by T. suis in its host and particularly, the resulting expulsion of worms is the focus of this thesis. Whipworms...

The arginine-ornithine antiporter ArcD contributes to biological fitness of Streptococcus suis

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Marcus eFulde

2014-08-01

Full Text Available The arginine-ornithine antiporter (ArcD is part of the Arginine Deiminase System (ADS, a catabolic, energy-providing pathway found in a variety of different bacterial species, including the porcine zoonotic pathogen Streptococcus suis. The ADS has recently been shown to play a role in the pathogenicity of S. suis, in particular in its survival in host cells. The contribution of arginine and arginine transport mediated by ArcD, however, has yet to be clarified. In the present study, we showed by experiments using [U-13C6]arginine as a tracer molecule that S. suis is auxotrophic for arginine and that bacterial growth depends on the uptake of extracellular arginine. To further study the role of ArcD in arginine metabolism, we generated an arcD-specific mutant strain and characterized its growth compared to the wild-type (WT strain, a virulent serotype 2 strain. The mutant strain showed a markedly reduced growth rate in chemically defined media supplemented with arginine when compared to the WT strain, indicating that ArcD promotes arginine uptake. To further evaluate the in vivo relevance of ArcD, we studied the intracellular bacterial survival of the arcD mutant strain in an epithelial cell culture infection model. The mutant strain was substantially attenuated, and its reduced intracellular survival rate correlated with a lower ability to neutralize the acidified environment. Based on these results, we propose that ArcD, by its function as an arginine-ornithine antiporter, is important for supplying arginine as substrate of the ADS and, thereby, contributes to biological fitness and virulence of S. suis in the host.

Mechanisms of Host-Pathogen Protein Complex Formation and Bacterial Immune Evasion of Streptococcus suis Protein Fhb.

Science.gov (United States)

Li, Xueqin; Liu, Peng; Gan, Shuzhen; Zhang, Chunmao; Zheng, Yuling; Jiang, Yongqiang; Yuan, Yuan

2016-08-12

Streptococcus suis serotype 2 (S. suis 2)-induced sepsis and meningitis are often accompanied by bacteremia. The evasion of polymorphonuclear leukocyte-mediated phagocytic clearance is central to the establishment of bacteremia caused by S. suis 2 and is facilitated by the ability of factor H (FH)-binding protein (Fhb) to bind FH on the bacterial surface, thereby impeding alternative pathway complement activation and phagocytic clearance. Here, C3b/C3d was found to bind to Fhb, along with FH, forming a large immune complex. The formation of this immune complex was mediated by domain II of Fhb via electrostatic and hydrophobic interactions, which, to our knowledge, is a new type of interaction. Interestingly, Fhb was found to be associated with the cell envelope and also present in the culture supernatant, where secreted Fhb inhibited complement activation via interactions with domain II, thereby enhancing antiphagocytic clearance by polymorphonuclear leukocytes. Thus, Fhb is a multifunctional bacterial protein, which binds host complement component C3 as well as FH and interferes with innate immune recognition in a secret protein manner. S. suis 2 therefore appears to have developed a new strategy to combat host innate immunity and enhance survival in host blood. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Mechanisms of Host-Pathogen Protein Complex Formation and Bacterial Immune Evasion of Streptococcus suis Protein Fhb*

Science.gov (United States)

Li, Xueqin; Liu, Peng; Gan, Shuzhen; Zhang, Chunmao; Zheng, Yuling; Jiang, Yongqiang; Yuan, Yuan

2016-01-01

Streptococcus suis serotype 2 (S. suis 2)-induced sepsis and meningitis are often accompanied by bacteremia. The evasion of polymorphonuclear leukocyte-mediated phagocytic clearance is central to the establishment of bacteremia caused by S. suis 2 and is facilitated by the ability of factor H (FH)-binding protein (Fhb) to bind FH on the bacterial surface, thereby impeding alternative pathway complement activation and phagocytic clearance. Here, C3b/C3d was found to bind to Fhb, along with FH, forming a large immune complex. The formation of this immune complex was mediated by domain II of Fhb via electrostatic and hydrophobic interactions, which, to our knowledge, is a new type of interaction. Interestingly, Fhb was found to be associated with the cell envelope and also present in the culture supernatant, where secreted Fhb inhibited complement activation via interactions with domain II, thereby enhancing antiphagocytic clearance by polymorphonuclear leukocytes. Thus, Fhb is a multifunctional bacterial protein, which binds host complement component C3 as well as FH and interferes with innate immune recognition in a secret protein manner. S. suis 2 therefore appears to have developed a new strategy to combat host innate immunity and enhance survival in host blood. PMID:27342778

Use of Antibiotics and Antimicrobial Resistance in Veterinary Medicine as Exemplified by the Swine Pathogen Streptococcus suis.

Science.gov (United States)

Seitz, Maren; Valentin-Weigand, Peter; Willenborg, Jörg

2016-01-01

Use of antimicrobial agents in veterinary medicine is essential to control infectious diseases, thereby keeping animals healthy and animal products safe for the consumer. On the other hand, development and spread of antimicrobial resistance is of major concern for public health. Streptococcus (S.) suis reflects a typical bacterial pathogen in modern swine production due to its facultative pathogenic nature and wide spread in the pig population. Thus, in the present review we focus on certain current aspects and problems related to antimicrobial use and resistance in S. suis as a paradigm for a bacterial pathogen affecting swine husbandry worldwide. The review includes (i) general aspects of antimicrobial use and resistance in veterinary medicine with emphasis on swine, (ii) genetic resistance mechanisms of S. suis known to contribute to bacterial survival under antibiotic selection pressure, and (iii) possible other factors which may contribute to problems in antimicrobial therapy of S. suis infections, such as bacterial persister cell formation, biofilm production, and co-infections. The latter shows that we hardly understand the complexity of factors affecting the success of antimicrobial treatment of (porcine) infectious diseases and underlines the need for further research in this field.

Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part II – Pathogenesis

Directory of Open Access Journals (Sweden)

Jacek Dutkiewicz

2018-03-01

Full Text Available Streptococcus suis is a re-emerging zoonotic pathogen that may cause severe disease, mostly meningitis, in pigs and in humans having occupational contact with pigs and pork, such as farmers, slaughterhose workers and butchers. The first stage of the pathogenic process, similar in pigs and humans, is adherence to and colonisation of mucosal and/or epithelial surface(s of the host. The second stage is invasion into deeper tissue and extracellular translocation of bacterium in the bloodstream, either free in circulation or attached to the surface of monocytes. If S. suis present in blood fails to cause fatal septicaemia, it is able to progress into the third stage comprising penetration into host’s organs, mostly by crossing the blood-brain barrier and/or blood–cerebrospinal fluid barrier to gain access to the central nervous system (CNS and cause meningitis. The fourth stage is inflammation that plays a key role in the pathogen esis of both systemic and CNS infections caused by S. suis . The pathogen may induce the overproduction of pro-inflammatory cytokines that cause septic shock and/or the recruitment and activation of different leukocyte populations, causing acute inflammation of the CNS. Streptococcus suis can also evoke – through activation of microglial cells, astrocytes and possibly other cell types – a fulminant inflammatory reaction of the brain which leads to intracranial complications, including brain oedema, increased intracranial pressure, cerebrovascular insults, and deafness, as a result of cochlear sepsis. In all stages of the pathogenic process, S. suis interacts with many types of immunocompetent host’s cells, such as polymorphonuclear leukocytes, mononuclear macrophages, lymphocytes, dendritic cells and microglia, using a range of versatile virulence factors for evasion of the innate and adaptive immune defence of the host, and for overcoming environmental stress. It is estimated that S. suis produces more than

A repA-based ELISA for discriminating cattle vaccinated with Brucella suis 2 from those naturally infected with Brucella abortus and Brucella melitensis.

Science.gov (United States)

Wang, Jing-Yu; Wu, Ning; Liu, Wan-Hua; Ren, Juan-Juan; Tang, Pan; Qiu, Yuan-Hao; Wang, Chi-Young; Chang, Ching-Dong; Liu, Hung-Jen

2014-01-01

The commonest ways of diagnosing brucellosis in animals include the Rose-Bengal plate agglutination test, the buffered plate agglutination test (BPA), the slide agglutination test, the complement fixation test, and the indirect enzyme linked immunosorbent assay (I-ELISA). However, these methods cannot discriminate the Brucella vaccine strain (Brucella suis strain 2; B. suis S2) from naturally acquired virulent strains. Of the six common Brucella species, Brucella melitensis, Brucella abortus, and B. suis are the commonest species occurring in China. To develop an ELISA assay that can differentiate between cows inoculated with B. suis S2 and naturally infected with B. abortus and B. melitensis, genomic sequences from six Brucella spp. (B. melitensis, B. abortus, B. suis, Brucella canis, Brucella neotomae and Brucella ovis) were compared using Basic Local Alignment Search Tool software. One particular gene, the repA-related gene, was found to be a marker that can differentiate B. suis from B. abortus and B. melitensis. The repA-related gene of B. suis was PCR amplified and subcloned into the pET-32a vector. Expressed repA-related protein was purified and used as an antigen. The repA-based ELISA was optimized and used as specific tests. In the present study, serum from animals inoculated with the B. suis S2 vaccine strain had positive repA-based ELISA results. In contrast, the test-positive reference sera against B. abortus and B. melitensis had negative repA-based ELISA results. The concordance rate between B. abortus antibody-negative (based on the repA-based ELISA) and the Brucella gene-positive (based on the 'Bruce ladder' multiplex PCR) was 100%. Therefore, the findings suggest that the repA-based ELISA is a useful tool for differentiating cows vaccinated with the B. suis S2 and naturally infected with B. abortus and B. melitensis. Copyright © 2014 Elsevier Ltd. All rights reserved.

Sanitary landfill groundwater monitoring report: First quarter 1997

Energy Technology Data Exchange (ETDEWEB)

Chase, J.A.

1997-05-01

This report contains analytical data for samples taken during first quarter 1997 from wells of the LFW series located at the Sanitary Landfill at the Savannah River Site (SRS). The data are submitted in reference to the Sanitary Landfill Operating permit (DWP-087A). The report presents monitoring results that equaled or exceeded the Safe Drinking Water Act final primary Drinking Water Standards (PDWS) or screening levels, established by the U.S. Environmental Protection Agency, the South Carolina final Primary Drinking Water Standard for lead, or the SRS flagging criteria. Wells LFW6R, LFW8R, LFW10A, LFW18, LFW21, and LFW23R were not sampled due to their proximity to the Sanitary Landfill Closure Cap activities. Wells LFW61D and LFW62D are Purge Water Containment Wells and contain mercury. These wells were not sampled since the purge water cannot be treated at the M-1 Air Stripper until the NPDES permit for the stripper is modified.

Postantibiotic effects and postantibiotic sub-MIC effects of tilmicosin, erythromycin and tiamulin on erythromycin-resistant Streptococcus suis.

Science.gov (United States)

Wang, Liping; Zhang, Yuanshu

2009-10-01

The postantibiotic effects (PAEs) and postantibiotic sub-MIC effects (PA SMEs) of tilmicosin, erythromycin and tiamulin on erythromycin-susceptible and erythromycin-resistant strains of Streptococcus suis (M phenotype) were investigated in vitro. Tilmicosin and tiamulin induced significantly longer PAE and PA SME against both erythromycin-susceptible and erythromycin-resistant strains than did erythromycin. The durations of PAE and PA SMEs were proportional to the concentrations of drugs used for exposure. The PA SMEs were substantially longer than PAEs on S. suis (P<0.05) regardless of the antimicrobial used for exposure. The results indicated that the PAE and PA SME could help in the design of efficient control strategies for infection especially caused by erythromycin-resistant S. suis and that they may provide additional valuable information for the rational drug use in clinical practice.

Development of a Chlamydia suis-specific antibody enzyme-linked immunosorbent assay based on the use of a B-cell epitope of the polymorphic membrane protein C.

Science.gov (United States)

De Puysseleyr, K; Kieckens, E; De Puysseleyr, L; Van den Wyngaert, H; Ahmed, B; Van Lent, S; Creasy, H H; Myers, G S A; Vanrompay, D

2018-04-01

Chlamydia suis infections lead to economic loss in the pork industry. Chlamydia suis infections could be successfully treated with tetracyclines until the appearance of a tetracycline resistant phenotype, which was acquired via horizontal gene transfer of the tet(C) gene. Given the importance of C. suis as a swine pathogen and as a recently emerged tetracycline resistant pathogen with zoonotic potential, our aim was to develop a sensitive C. suis-specific antibody ELISA based on the polymorphic membrane proteins (Pmps). Chlamydia Pmps are important virulence factors and candidate antigens for serodiagnosis. We identified nine Pmps (PmpA to I) in C. suis strain MD56 using a recently developed Hidden-Markov model. PmpC was the most promising candidate for the development of a C. suis-specific antibody ELISA as the protein was absent in C. abortus, C. pecorum and C. psittaci which also infect pigs and as the protein contained C. suis-specific amino acid regions, absent in C. trachomatis PmpC. We identified an immunodominant B-cell epitope in C. suis PmpC using experimental porcine sera. The sensitivity and specificity of the PmpC ELISA was compared to the complement fixation test (CFT) and to a recombinant MOMP ELISA using experimental sera. The PmpC ELISA detected all positive control sera and was in contrast to CFT and the rMOMP ELISA 100% C. suis specific as positive control sera against other Chlamydia species did not react in the PmpC ELISA. The test was successfully validated using slaughterhouse sera and sera from clinically affected pigs. The PmpC ELISA could assist in diminishing the spread of C. suis infections in the pork industry. © 2018 Blackwell Verlag GmbH.

Characterization of the Pivotal Carbon Metabolism of Streptococcus suis Serotype 2 under ex Vivo and Chemically Defined in Vitro Conditions by Isotopologue Profiling*

Science.gov (United States)

Willenborg, Jörg; Huber, Claudia; Koczula, Anna; Lange, Birgit; Eisenreich, Wolfgang; Valentin-Weigand, Peter; Goethe, Ralph

2015-01-01

Streptococcus suis is a neglected zoonotic pathogen that has to adapt to the nutritional requirements in the different host niches encountered during infection and establishment of invasive diseases. To dissect the central metabolic activity of S. suis under different conditions of nutrient availability, we performed labeling experiments starting from [13C]glucose specimens and analyzed the resulting isotopologue patterns in amino acids of S. suis grown under in vitro and ex vivo conditions. In combination with classical growth experiments, we found that S. suis is auxotrophic for Arg, Gln/Glu, His, Leu, and Trp in chemically defined medium. De novo biosynthesis was shown for Ala, Asp, Ser, and Thr at high rates and for Gly, Lys, Phe, Tyr, and Val at moderate or low rates, respectively. Glucose degradation occurred mainly by glycolysis and to a minor extent by the pentose phosphate pathway. Furthermore, the exclusive formation of oxaloacetate by phosphoenolpyruvate (PEP) carboxylation became evident from the patterns in de novo synthesized amino acids. Labeling experiments with S. suis grown ex vivo in blood or cerebrospinal fluid reflected the metabolic adaptation to these host niches with different nutrient availability; however, similar key metabolic activities were identified under these conditions. This points at the robustness of the core metabolic pathways in S. suis during the infection process. The crucial role of PEP carboxylation for growth of S. suis in the host was supported by experiments with a PEP carboxylase-deficient mutant strain in blood and cerebrospinal fluid. PMID:25575595

Use of Tetravalent Galabiose for Inhibition of Streptococcus Suis Serotype 2 Infection in a Mouse Model

Directory of Open Access Journals (Sweden)

Karen A. Krogfelt

2013-04-01

Full Text Available Streptococcus suis is an important swine pathogen associated with a variety of infections such as meningitis, arthritis and septicemia. The bacterium is zoonotic and has been found to cause meningitis especially in humans occupationally exposed to infected pigs. Since adhesion is a prerequisite for colonization and subsequent infection, anti-adhesion treatment seems a natural alternative to traditional treatment with antibiotics. In order to optimize the inhibitory potency a multivalency approach was taken in the inhibitor design. A synthetic tetravalent galabiose compound was chosen which had previously shown promising anti-adhesion effects with S. suis in vitro. The aim of this study was to evaluate the in vivo effects of the compound using an infection peritonitis mouse model. As such S. suis serotype 2 infection and treatment were tested in vivo and the effects were compared to the effect of treatment with penicillin.

Hanford 200 area (sanitary) waste water system

International Nuclear Information System (INIS)

Danch, D.A.; Gay, A.E.

1994-09-01

The US Department of Energy (DOE) Hanford Site is located in southeastern Washington State. The Hanford Site is approximately 1,450 sq. km (560 sq. mi) of semiarid land set aside for activities of the DOE. The reactor fuel processing and waste management facilities are located in the 200 Areas. Over the last 50 years at Hanford dicard of hazardous and sanitary waste water has resulted in billions of liters of waste water discharged to the ground. As part of the TPA, discharges of hazardous waste water to the ground and waters of Washington State are to be eliminated in 1995. Currently sanitary waste water from the 200 Area Plateau is handled with on-site septic tank and subsurface disposal systems, many of which were constructed in the 1940s and most do not meet current standards. Features unique to the proposed new sanitary waste water handling systems include: (1) cost effective operation of the treatment system as evaporative lagoons with state-of-the-art liner systems, and (2) routing collection lines to avoid historic contamination zones. The paper focuses on the challenges met in planning and designing the collection system

Sanitary Landfill Groundwater Monitoring Report, Second Quarter 1999

Energy Technology Data Exchange (ETDEWEB)

Chase, J.

1999-07-29

This report contains analytical data for samples taken during Second Quarter 1999 from wells of the LFW series located at the Sanitary Landfill at the Savannah River Site. The data are submitted in reference to the Sanitary Landfill Operating Permit. The report presents monitoring results that equaled or exceeded the Safe Drinking Water Act final Primary Drinking Water Standards or screening levels, established by the US Environmental Protection Agency, the South Carolina final Primary Drinking Water Standard for lead, or the SRS flagging criteria.

Sanitary landfill groundwater monitoring report, Third Quarter 1999

Energy Technology Data Exchange (ETDEWEB)

Chase, J.

1999-12-08

This report contains analytical data for samples taken during Third Quarter 1999 from wells of the LFW series located at the Sanitary Landfill at the Savannah River Site. The data are submitted in reference to the Sanitary Landfill Operating Permit. The report presents monitoring results that equaled or exceeded the Safe Drinking Water Act final Primary Drinking Water Standards or screening levels, established by the U.S. Environmental Protection Agency, the South Carolina final Primary Drinking Water Standard for lead, or the SRS flagging criteria.

Sanitary landfill groundwater monitoring report, Third Quarter 1999

International Nuclear Information System (INIS)

Chase, J.

1999-01-01

This report contains analytical data for samples taken during Third Quarter 1999 from wells of the LFW series located at the Sanitary Landfill at the Savannah River Site. The data are submitted in reference to the Sanitary Landfill Operating Permit. The report presents monitoring results that equaled or exceeded the Safe Drinking Water Act final Primary Drinking Water Standards or screening levels, established by the U.S. Environmental Protection Agency, the South Carolina final Primary Drinking Water Standard for lead, or the SRS flagging criteria

Trichuris suis ova therapy for allergic rhinitis

DEFF Research Database (Denmark)

Bager, Peter; Arnved, John; Rønborg, Steen

2010-01-01

Parasitic helminth infections can protect against allergic airway inflammation in experimental models and have been associated with a reduced risk of atopy and a reduced course of asthma in some observational studies. Although no clinical evidence exists to support the use of helminth therapy...... for allergic disease, the helminth Trichuris suis has demonstrated efficacy in treatment of inflammatory bowel disease....

Sanitary landfill groundwater monitoring report. Third quarter 1995

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-11-01

This report contains analytical data for samples taken during third quarter 1995 from wells of the LFW series located at the Sanitary Landfill at the Savannah River Site (SRS). The data are submitted in reference to the Sanitary Landfill Operating Permit (DWP-087A). The report presents monitoring results that equaled or exceeded the Safe Drinking Water Act final Primary Drinking Water Standards (PDWS) or screening levels, established by the U.S. Environmental Protection Agency, the South Carolina final Primary Drinking Water Standard for lead, or the SRS flagging criteria.

Sanitary landfill groundwater monitoring report. Third quarter 1995

International Nuclear Information System (INIS)

1995-11-01

This report contains analytical data for samples taken during third quarter 1995 from wells of the LFW series located at the Sanitary Landfill at the Savannah River Site (SRS). The data are submitted in reference to the Sanitary Landfill Operating Permit (DWP-087A). The report presents monitoring results that equaled or exceeded the Safe Drinking Water Act final Primary Drinking Water Standards (PDWS) or screening levels, established by the U.S. Environmental Protection Agency, the South Carolina final Primary Drinking Water Standard for lead, or the SRS flagging criteria

Postantibiotic effects and postantibiotic sub-MIC effects of tilmicosin, erythromycin and tiamulin on erythromycin-resistant Streptococcus suis

Directory of Open Access Journals (Sweden)

Liping Wang

2009-12-01

Full Text Available The postantibiotic effects (PAEs and postantibiotic sub-MIC effects (PA SMEs of tilmicosin, erythromycin and tiamulin on erythromycin-susceptible and erythromycin-resistant strains of Streptococcus suis (M phenotype were investigated in vitro. Tilmicosin and tiamulin induced significantly longer PAE and PA SME against both erythromycin-susceptible and erythromycin-resistant strains than did erythromycin. The durations of PAE and PA SMEs were proportional to the concentrations of drugs used for exposure. The PA SMEs were substantially longer than PAEs on S. suis (P<0.05 regardless of the antimicrobial used for exposure. The results indicated that the PAE and PA SME could help in the design of efficient control strategies for infection especially caused by erythromycin-resistant S. suis and that they may provide additional valuable information for the rational drug use in clinical practice.

Correlation between PFGE Groups and mrp/epf/sly Genotypes of Human Streptococcus suis Serotype 2 in Northern Thailand

Directory of Open Access Journals (Sweden)

Prasit Tharavichitkul

2014-01-01

Full Text Available Streptococcus suis infection is a severe zoonotic disease commonly found in Northern Thailand where people often consume raw pork and/or pig’s blood. The most frequent clinical presentations are meningitis, sepsis, and endocarditis with higher rate of mortality and hearing loss sequelae. To clarify the correlation between pulsed-field gel electrophoresis (PFGE groups and mrp/epf/sly genotypes of S. suis serotype 2, 62 patient and 4 healthy pig isolates from Northern Thailand were studied. By PFGE analysis, at 66% homology, most human isolates (69.4% and 1 pig isolate were in group A, whereas 14.5% of human isolates and 3 out of 4 pig isolates were in group D. According to mrp/epf/sly genotypes, 80.6% of human isolates were identified in mrp+epf−sly− and only 12.9% were in mrp−epf−sly+ genotypes; in contrast, 1 and 3 pig isolates were detected in these two genotypes, respectively. Interestingly, all isolates of S. suis serotype 2 classified in PFGE groups A, B, and E were set in mrp+epf−sly− genotypes. These data show a close correlation between PFGE groups and mrp/epf/sly genotypes of human S. suis serotype 2.

Correlation between PFGE Groups and mrp/epf/sly Genotypes of Human Streptococcus suis Serotype 2 in Northern Thailand.

Science.gov (United States)

Tharavichitkul, Prasit; Wongsawan, Kanreuthai; Takenami, Naoki; Pruksakorn, Sumalee; Fongcom, Achara; Gottschalk, Marcelo; Khanthawa, Banyong; Supajatura, Volaluk; Takai, Shinji

2014-01-01

Streptococcus suis infection is a severe zoonotic disease commonly found in Northern Thailand where people often consume raw pork and/or pig's blood. The most frequent clinical presentations are meningitis, sepsis, and endocarditis with higher rate of mortality and hearing loss sequelae. To clarify the correlation between pulsed-field gel electrophoresis (PFGE) groups and mrp/epf/sly genotypes of S. suis serotype 2, 62 patient and 4 healthy pig isolates from Northern Thailand were studied. By PFGE analysis, at 66% homology, most human isolates (69.4%) and 1 pig isolate were in group A, whereas 14.5% of human isolates and 3 out of 4 pig isolates were in group D. According to mrp/epf/sly genotypes, 80.6% of human isolates were identified in mrp (+) epf (-) sly (-) and only 12.9% were in mrp (-) epf (-) sly (+) genotypes; in contrast, 1 and 3 pig isolates were detected in these two genotypes, respectively. Interestingly, all isolates of S. suis serotype 2 classified in PFGE groups A, B, and E were set in mrp (+) epf (-) sly (-) genotypes. These data show a close correlation between PFGE groups and mrp/epf/sly genotypes of human S. suis serotype 2.

Pre-absorbed immunoproteomics: a novel method for the detection of Streptococcus suis surface proteins.

Directory of Open Access Journals (Sweden)

Wei Zhang

Full Text Available Streptococcus suis serotype 2 (SS2 is a zoonotic pathogen that can cause infections in pigs and humans. Bacterial surface proteins are often investigated as potential vaccine candidates and biomarkers of virulence. In this study, a novel method for identifying bacterial surface proteins is presented, which combines immunoproteomic and immunoserologic techniques. Critical to the success of this new method is an improved procedure for generating two-dimensional electrophoresis gel profiles of S. suis proteins. The S. suis surface proteins identified in this study include muramidase-released protein precursor (MRP and an ABC transporter protein, while MRP is thought to be one of the main virulence factors in SS2 located on the bacterial surface. Herein, we demonstrate that the ABC transporter protein can bind to HEp-2 cells, which strongly suggests that this protein is located on the bacterial cell surface and may be involved in pathogenesis. An immunofluorescence assay confirmed that the ABC transporter is localized to the bacterial outer surface. This new method may prove to be a useful tool for identifying surface proteins, and aid in the development of new vaccine subunits and disease diagnostics.

The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis

DEFF Research Database (Denmark)

Sørensen, Nanna Skall; Tegtmeier, C.; Andresen, Lars Ole

2006-01-01

The pig acute phase protein (APP) response to experimental Streptococcus suis (S. suis) infection was mapped by the measurement of the positive APPs C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp) and major acute phase protein (pig-MAP) and the negative APPs albumin...... and apolipoprotein (Apo) A-I. The aim was to elucidate the differences in the acute phase behaviour of the individual APPs during a typical bacterial septicaemic, infection. Pigs were inoculated subcutaneously with live S. suis serotype 2 and blood was sampled before and on various days post inoculation (p...... the experiment with maximum levels around 10 times the day 0-levels, and pig-MAP was elevated on days 1-12 p.i. with peak levels of around seven times the day 0-levels. Apo A-I was decreased from days 1 to 8 and showed minimum levels of about 40% of day 0-levels around 1-2 days p.i. No clear pattern of changes...

Detailed test plan on co-disposal of deminumum levels of LLRW and sanitary waste

International Nuclear Information System (INIS)

Chian, E.S.K.

1984-01-01

The major objectives of this investigation are: (1) to study the attenuation mechanisms for radionuclides (LLRW) in simulated sanitary landfills with and without leachate recycle; and (2) to determine the complexation potential of ligands (viz., sulfides, humic substances) present in the leachate during different stages of landfill stabilization. Since the major emphasis of this research is on containment and isolation of LLRW within a sanitary landfill the following key elements have been incorporated into the design of the simulated pilot-scale sanitary landfills: (1) containment of LLRW within the landfills by placing the municipal refuse and LLRW in polymeric membrane-lined cells (30-mil, HDA, Gundline liner, Gundle, TX) with a leachate collection system; (2) enhancement of stabilization by leachate recycle; and (3) recovery of the gas produced from the sanitary landfills. The proposed research program addresses the fate of LLRW in municipal sanitary landfills thereby, establishing a data base to predict radionuclide pathways in sanitary landfills and to aid in assessing the environmental impacts of codisposal of LLRW within sanitary landfills. The outcome of this study may also serve to alleviate the current concern of unnecessary filling of limited space in existing licensed shallow-land burial sites. 4 figures, 1 table

Excreted/secreted Trichuris suis products reduce barrier function and suppress inflammatory cytokine production of intestinal epithelial cells

DEFF Research Database (Denmark)

Hiemstra, I. H.; Klaver, E. J.; Vrijland, K.

2014-01-01

The administration of helminths is considered a promising strategy for the treatment of autoimmune diseases due to their immunomodulatory properties. Currently, the application of the helminth Trichuris suis as a treatment for Crohn's disease is being studied in large multi-center clinical trials....... The intestinal epithelium forms an efficient barrier between the intestinal lumen containing the microbial flora and helminths, and dendritic cells (DCs) present in the lamina propria that determine the TH response. Here, we investigated how excreted/secreted (E/S) products of T. suis affect the barrier function...... of intestinal epithelial cells (IECs) in order to reach the DCs and modulate the immune response. We show that T. suis E/S products reduce the barrier function and the expression of the tight junction proteins EMP-1 and claudin-4 in IEC CMT93/69 monolayers in a glycan-dependent manner. This resulted...

[Evaluation of the sanitary-and-epidemiological hazard of solid garbage in Astana].

Science.gov (United States)

Gumarova, Zh Zh; Bekshin, Zh M; Aushakhmetova, Z T

2008-01-01

According to the national plan of actions on environmental protection, industrial garbage recycling is to be introduced in Almaty and Astana for the sustainable development of the Republic of Kazakhstan. Integrated assessment of the hazard of garage is made by the sanitary-and-chemical and sanitary-and-epidemiological indices to provide the hygienic and ecological reliability of a procedure for neutralization and utilization of solid garbage (SG). According to the data obtained, Astana SG Astana in summer is characterized by the high total level of bacterial contamination. The indices of microbial contamination of SG and soil near the dustbins correlate with the density of population and the maturity of an infrastructure. Comparison of the sanitary-and-epidemiological indices of different types of SG (wastes from housing facilities, wholesale and retail outlays, and education, culture, and entertainment institutions) revealed no significant differences. According to the sanitary-and-helmintological indices, the Astana soil should be classified as pure (noninvasive). Involvement of SG into industrial recycling should be accompanied by a hygienic assessment of the hazard of waste and the reliability of used technologies in the context of warning and on-going sanitary surveillance.

Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O : 9 after natural or experimental infection in pigs

DEFF Research Database (Denmark)

Jungersen, Gregers; Sørensen, Vibeke; Giese, Steen Bjørck

2006-01-01

with responses of B. suis biovar 2-inoculated pigs. FPSR were limited to 2-9 weeks post-YeO:9 inoculation, while B. suis-infected pigs were test-positive throughout the 21-week period of investigation. Although YeO:9-inoculated pigs exhibited FPSR in Brucella tests for a limited period of time, the serological...

Meningitis por Streptococcus suis en un paciente inmunocompetente Streptococcus suis meningitis in an immunocompetent patient

Directory of Open Access Journals (Sweden)

A. Nagel

2008-09-01

Full Text Available Se describe un caso de meningitis por Streptococcus suis en un paciente inmunocompetente. Presentaba astenia, debilidad generalizada, fiebre (39 °C, vómitos, deterioro del sensorio y desorientación témporo-espacial. Los cultivos de sangre (2/2 y de líquido cefalorraquídeo fueron positivos. La identificación preliminar se realizó utilizando las pruebas bioquímicas convencionales y fue completada en el Servicio Bacteriología Especial del INEI-ANLIS "Dr. Carlos G. Malbrán". Se comenzó el tratamiento con ampicilina y ceftriaxona. El microorganismo aislado demostró sensibilidad a ampicilina, cefotaxima y vancomicina. El paciente evolucionó favorablemente, pero se comprobó leve hipoacusia. Reingresó a los 4 meses con marcha atáxica, anacusia en oído izquierdo e hipoacusia en oído derecho. Continúa con seguimiento neurológico y audiométrico. Retrospectivamente se constató el contacto del paciente con cerdos. Se destaca la importancia de la anamnesis para alertar la sospecha de este agente etiológico en meningitis y bacteriemias.A case of Streptococcus suis meningitis is described in an immunocompetent patient presenting asthenia, general weakness, fever, vomiting, sensory deterioration and temporospatial disorder. The cerebrospinal fluid and two blood cultures (2/2 bottles were positive. The isolate was preliminary identified by conventional biochemical tests, and the identification was completed at the Special Bacteriology Service of INEI-ANLIS "Dr. Carlos G. Malbrán". Ampicillin and ceftriaxone treatment was initiated. The isolate was susceptible to ampicillin, cefotaxime and vancomycin. The patient experienced a good outcome but suffered hearing loss. However, after four months he returned with walking ataxia, deafness in his left ear, and hearing loss in the right ear. The patient’s retrospective exposure to pigs had been verified. It is important to evaluate predisposing and epidemiologic factors in order to alert about

Comparison of the sanitary effects of energy chains. Methodological aspects

International Nuclear Information System (INIS)

Fagnani, F.

1979-01-01

Beyond technical and economical matters, the development of an industrial technology involves more or less numerous indirect consequences. From this viewpoint, the author analysis the methodological problems raised in evaluating the sanitary and ecological problems of the different energy-producing lines and considers successively the matter of technical interdependences, protection and safety regulations and selection of sites, classification of risks and measuring problems in relation to sanitary effects [fr

Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I - Epidemiology.

Science.gov (United States)

Dutkiewicz, Jacek; Sroka, Jacek; Zając, Violetta; Wasiński, Bernard; Cisak, Ewa; Sawczyn, Anna; Kloc, Anna; Wójcik-Fatla, Angelina

2017-12-23

Streptococcus suis (ex Elliot 1966, Kilpper-Bälz & Schleifer 1987) is a facultatively anaerobic Gram-positive ovoid or coccal bacterium surrounded by a polysaccharide capsule. Based on the antigenic diversity of the capsule, S. suis strains are classified serologically into 35 serotypes. Streptococcus suis is a commensal of pigs, commonly colonizing their tonsils and nasal cavities, mostly in weaning piglets between 4-10 weeks of age. This species occurs also in cattle and other mammals, in birds and in humans. Some strains, mostly those belonging to serotype 2, are also pathogenic for pigs, as well as for other animals and humans. Meningitis is the primary disease syndrome caused by S. suis, both in pigs and in humans. It is estimated that meningitis accounted for 68.0% of all cases of human disease reported until the end of 2012, followed by septicaemia (including life-threatening condition described as 'streptococcal toxic shock-like syndrome' - STSLS), arthritis, endocarditis, and endophthalmitis. Hearing loss and/or ves tibular dysfunction are the most common sequelae after recovery from meningitis caused by S. suis, occurring in more than 50% of patients. In the last two decades, the number of reported human cases due to S. suis has dramatically increased, mostly due to epidemics recorded in China in 1998 and 2005, and the fulminant increase in morbidity in the countries of south-eastern Asia, mostly Vietnam and Thailand. Out of 1,642 cases of S. suis infections identified between 2002-2013 worldwide in humans, 90.2% occurred in Asia, 8.5% in Europe and 1.3% in other parts of the globe. The human disease has mostly a zoonotic and occupational origin and occurs in pig breeders, abattoir workers, butchers and workers of meat processing facilities, veterinarians and meat inspectors. Bacteria are transmitted to workers by close contact with pigs or pig products, usually through contamination of minor cuts or abrasions on skin of hands and/or arms, or by pig bite

Electric utilities strategies in final energy markets; Nuove strategie d'impresa sui mercati finali dell'energia

Energy Technology Data Exchange (ETDEWEB)

Bianchi, A. [RIE S.r.L., Bologna (Italy)

2000-07-01

In rapidly changing markets, electric utilities pay growing attention to customers and service. They are aware that competition needs strategies capable of transforming and strengthening the privileged position resulting from the knowledge of the market. Moreover, this aspect is the link between different value chains to describe new multi utility approaches. [Italian] In mercati in rapida evoluzione, cresce nelle compagnie energetiche l'attenzione al cliente e al servizio; la concorrenza va affrontata con strategie di trasformazione e rafforzamento della posizione di privilegio che la conoscenza del mercato offre. Questo elemento rappresenta poi il ponte tra catene del valore diverse, verso un nuovo approccio multi-servizi.

Narratives of Public Health in Dickens's Journalism: The Trouble with Sanitary Reform.

Science.gov (United States)

Smith, Ralph F

2015-01-01

Although Dickens is still known as having been a highly visible supporter of England's well-known nineteenth-century sanitary movement, he became, in fact, deeply troubled by many of this movement's fundamental tenets, as evidenced by journal narratives on fever that he edited and wrote in the mid-nineteenth century. Rather than water and sewer engineering works and a sanitary regime policed by government agencies as envisaged by Edwin Chadwick and other sanitary reformers, Dickens's view by 1855 was that only a massive erasure of the existing social and political systems and their replacement by an utterly new infrastructure would suffice.

[Ways of urban sanitary and epidemiological well-being management].

Science.gov (United States)

Kreĭmer, M A

2010-01-01

The scientific rationale for preventive measures based on sanitary-and-epidemiological surveillance on environmental objects is considered. The sizes of functional zones and space for various types of communal services and amenities and leisure are regulated to ensure good urban vital activities. Multistorey housing causes an increase in the number of negative factors per area units and in their impact on health. A proposal has been made for the standardization of the ranges of urban population upsurge and size, by using the sanitary-and-hygienic rules and norms rather than climatic parameters. A criterion system for assessing the data of statistical observations has been substantiated and 5 levels of analysis and managerial decision-making have been proposed. Cause-and-effect relations may be determined for the parameters of the second level; models of program-oriented studies for the third level, only sanitary-and-epidemiological surveillance is possible for the fourth and fifth levels. The space planning scheme must provide for water supply reserves, generation areas for pure air coming into the town, and waste disposal areas. The general layout may use statistical observation parameters characterizing the second level of occurrence of negative phenomena. The statistical observation parameters characterizing the third and fourth levels of occurrence of negative phenomena may be used for municipal improvements and sanitary maintenance. These characterizing the fourth and fifth level may be used for prevention in therapeutic-and-prophylactic institutions.

Prevalence and pathogenicity of Cryptosporidium suis in pre- and post-weaned pigs

Czech Academy of Sciences Publication Activity Database

Vítovec, J.; Hamadejová, K.; Landová, L.; Kváč, Martin; Květoňová, Dana; Sak, Bohumil

2006-01-01

Roč. 53, č. 5 (2006), s. 239-243 ISSN 0931-1793 R&D Projects: GA ČR GA524/05/0992 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cryptosporidium suis * piglets * pathogenicity Subject RIV: EG - Zoology Impact factor: 1.356, year: 2006

Evolution and Diversity of the Antimicrobial Resistance Associated Mobilome in Streptococcus suis: A Probable Mobile Genetic Elements Reservoir for Other Streptococci.

Science.gov (United States)

Huang, Jinhu; Ma, Jiale; Shang, Kexin; Hu, Xiao; Liang, Yuan; Li, Daiwei; Wu, Zuowei; Dai, Lei; Chen, Li; Wang, Liping

2016-01-01

Streptococcus suis is a previously neglected, newly emerging multidrug-resistant zoonotic pathogen. Mobile genetic elements (MGEs) play a key role in intra- and interspecies horizontal transfer of antimicrobial resistance (AMR) determinants. Although, previous studies showed the presence of several MGEs, a comprehensive analysis of AMR-associated mobilome as well as their interaction and evolution has not been performed. In this study, we presented the AMR-associated mobilome and their insertion hotspots in S. suis . Integrative conjugative elements (ICEs), prophages and tandem MGEs were located at different insertion sites, while 86% of the AMR-associated MGEs were inserted at rplL and rum loci. Comprehensive analysis of insertions at rplL and rum loci among four pathogenic Streptococcus species ( Streptococcus agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes , and S. suis ) revealed the existence of different groups of MGEs, including Tn5252, ICE Sp 1108, and TnGBS2 groups ICEs, Φm46.1 group prophage, ICE_ICE and ICE_prophage tandem MGEs. Comparative ICE genomics of ICE Sa 2603 family revealed that module exchange and acquisition/deletion were the main mechanisms in MGEs' expansion and evolution. Furthermore, the observation of tandem MGEs reflected a novel mechanism for MGE diversity. Moreover, an in vitro competition assay showed no visible fitness cost was observed between different MGE-carrying isolates and a conjugation assay revealed the transferability of ICE Sa 2603 family of ICEs. Our statistics further indicated that the prevalence and diversity of MGEs in S. suis is much greater than in other three species which prompted our hypothesis that S. suis is probably a MGEs reservoir for other streptococci. In conclusion, our results showed that acquisition of MGEs confers S. suis not only its capability as a multidrug resistance pathogen, but also represents a paradigm to study the modular evolution and matryoshkas of MGEs.

Evolution and diversity of the antimicrobial resistance associated mobilome in Streptococcus suis: a probable mobile genetic elements reservoir for other streptococci

Directory of Open Access Journals (Sweden)

Jinhu Huang

2016-10-01

Full Text Available Streptococcus suis is a previously neglected, newly emerging multidrug-resistant zoonotic pathogen. Mobile genetic elements (MGEs play a key role in intra- and interspecies horizontal transfer of antimicrobial resistance (AMR determinants. Although previous studies showed the presence of several MGEs, a comprehensive analysis of AMR-associated mobilome as well as their interaction and evolution has not been performed. In this study, we presented the AMR-associated mobilome and their insertion hotspots in S. suis. Integrative conjugative elements (ICEs, prophages and tandem MGEs were located at different insertion sites, while 86% of the AMR-associated MGEs were inserted at rplL and rum loci. Comprehensive analysis of insertions at rplL and rum loci among four pathogenic Streptococcus species (Streptococcus agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and S. suis revealed the existence of different groups of MGEs, including Tn5252, ICESp1108, and TnGBS2 groups ICEs, Φm46.1 group prophage, ICE_ICE and ICE_prophage tandem MGEs. Comparative ICE genomics of ICESa2603 family revealed that module exchange and acquisition/deletion were the main mechanisms in MGEs’ expansion and evolution. Furthermore, the observation of tandem MGEs reflected a novel mechanism for MGE diversity. Moreover, an in vitro competition assay showed no visible fitness cost was observed between different MGE-carrying isolates and a conjugation assay revealed the transferability of ICESa2603 family of ICEs. Our statistics further indicated that the prevalence and diversity of MGEs in S. suis is much greater than in other three species which prompted our hypothesis that S. suis is probably a MGEs reservoir for other streptococci. In conclusion, our results showed that acquisition of MGEs confers S. suis not only its capability as a multidrug resistance pathogen, but also represents a paradigm to study the modular evolution and matryoshkas of MGEs.

The SWOT Analysis of a Romanian Post-Sanitary Institution in Romania

Directory of Open Access Journals (Sweden)

Cristina Iorga

2016-01-01

Starting from a comprehensive research (literature review, the paper proposes the realization of aSWOT analysis within a post-sanitary school, which aims at emphasizing the main advantages of apost-secondary schools perceived by students and discovering major development opportunities forthe quality of services offered by it, as a premise of the improvement of the sanitary schools, andthus, the health system in Romania.

Biogas of sanitary fillers

International Nuclear Information System (INIS)

Serrano Camacho, Ciro

2007-01-01

The author proposes a methodology for the preliminary estimation of the energetic potential and environmental improvement derivates of the implementation of these technologies that allows to make the first estimative of biogas generation of sanitary fillers with base in the results of the simulation of three predictive model: One Mexican, other denominated Scholl-Canyon of North American origin and the designed by the EPA. The three models use different versions and constants for a differential equation of degradation of first degree

Effluent and sanitary sewer monitors

International Nuclear Information System (INIS)

Stanchi, L.; Vasey, M.R.

1977-03-01

Two similar instruments that monitor the liquid wastes from the plutonium facility are described. The operation of the two instruments is completely automatic and performs a continuous surveillance in the frame of Nuclear Safeguards. One instrument controls the liquids from the facility and the other checks the sanitary sewer wastes. Both have self-diagnosing capabilities and take automatic actions in case of abnormal occurrences

Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I – Epidemiology

Directory of Open Access Journals (Sweden)

Jacek Dutkiewicz

2017-12-01

Full Text Available Streptococcus suis (ex Elliot 1966, Kilpper-Bälz & Schleifer 1987 is a facultatively anaerobic Gram-positive ovoid or coccal bacterium surrounded by a polysaccharide capsule. Based on the antigenic diversity of the capsule, S. suis strains are classified serologically into 35 serotypes. Streptococcus suis is a commensal of pigs, commonly colonizing their tonsils and nasal cavities, mostly in weaning piglets between 4–10 weeks of age. This species occurs also in cattle and other mammals, in birds and in humans. Some strains, mostly those belonging to serotype 2, are also pathogenic for pigs, as well as for other animals and humans. Meningitis is the primary disease syndrome caused by S. suis , both in pigs and in humans. It is estimated that meningitis accounted for 68.0% of all cases of human disease reported until the end of 2012, followed by septicaemia (including life-threatening condition described as ‘streptococcal toxic shock-like syndrome’ – STSLS, arthritis, endocarditis, and endophthalmitis. Hearing loss and/or ves tibular dysfunction are the most common sequelae after recovery from meningitis caused by S. suis , occurring in more than 50% of patients. In the last two decades, the number of reported human cases due to S. suis has dramatically increased, mostly due to epidemics recorded in China in 1998 and 2005, and the fulminant increase in morbidity in the countries of south-eastern Asia, mostly Vietnam and Thailand. Out of 1,642 cases of S. suis infections identified between 2002–2013 worldwide in humans, 90.2% occurred in Asia, 8.5% in Europe and 1.3% in other parts of the globe. The human disease has mostly a zoonotic and occupational origin and occurs in pig breeders, abattoir workers, butchers and workers of meat processing facilities, veterinarians and meat inspectors. Bacteria are transmitted to workers by close contact with pigs or pig products, usually through contamination of minor cuts or abrasions on skin of

30 CFR 71.402 - Minimum requirements for bathing facilities, change rooms, and sanitary flush toilet facilities.

Science.gov (United States)

2010-07-01

... nonirritating cleansing agent shall be provided for use at each shower. (2) Sanitary flush toilet facilities. (i..., change rooms, and sanitary flush toilet facilities. 71.402 Section 71.402 Mineral Resources MINE SAFETY... Rooms, and Sanitary Flush Toilet Facilities at Surface Coal Mines § 71.402 Minimum requirements for...

PROPOSAL OF SANITARY MANAGEMENT OF EDIBLE ECHINODERMS IN SARDINIA

Directory of Open Access Journals (Sweden)

G. Terrosu

2011-01-01

Full Text Available Sea urchin (Paracentrotus lividus is an edible echinoderm very common in the Mediterranean sea. In the Sardinian gastronomic tradition it represents a product very used in some periods of the year, but in practice the sanitary controls by the competent authorities are very difficult. The Reg. (EC n. 853/2004 provides that, as regards as the control on production, echinoderms are assimilable to live bivalve molluscs, with the exception of the provisions on purification. In this work a proposal for the sanitary management of the phases of gathering, transport and selling of the sea urchins has been studied.

[Sanitary control of food in Mexico city].

Science.gov (United States)

Sartí-Gutiérrez, E J; Parrilla-Cerrillo, C; Vázquez-Barojas, S; Kawashima-Hashimoto, L; Farías-Rodríguez, A; García-Juárez, C

1989-01-01

This work was undertaken as to avoid health risks to tourism in the "XIIth Soccer World Cup, Mexico 86". The above project was carried out through proper quality control of foods, drinking water as well as a definite care of personal hygiene of all those involved in the catering business (132 restaurants were included). The mentioned report informs only of the work of the National Public Health Laboratory in the Sanitary Epidemiological Program. The following products were analyzed: 133 water samples, 272 animated and nonliving surfaces, and 399 foods. Twenty seven percent of water samples were rejected, as well as 85% of the different surfaces and 60% of the food samples. High counts of mesophilic aerobics, followed by coliforms, S. aureus and Salmonella sp., were the principal specimens encountered. Therefore, it was suggested the permanent development of a sanitary epidemiological program.

[The sanitary and hygienic state of solid garbage burial grounds in the stages of a life cycle].

Science.gov (United States)

Zomarev, A M; Vaĭsman, Ia I; Zaĭtseva, T A; Glushankova, I S

2010-01-01

The purpose of the study was to assess the sanitary-and-hygienic state of solid garbage (SG) burial grounds in the Perm Territory in different stage of a life cycle. This paper presents the results of the study of deposited waste, forming dump soil, and SG ground emissions by general sanitary and sanitary-microbiological parameters and their effect on environmental objects. The performed studies of the sanitary-and-hygienic situation on some grounds of the Perm Territory suggest that there is a need for setting up a system for sanitary-and-monitoring of SG ground and for elaborating engineering, organizational, and prophylactic measures to assure the sanitary-and-hygienic safety of objects and to control the quality and quantity of waste to be buried and the currents of emissions (ground body degassing, filtrating sewage drainage and purification).

21 CFR 129.35 - Sanitary facilities.

Science.gov (United States)

2010-04-01

... HUMAN CONSUMPTION PROCESSING AND BOTTLING OF BOTTLED DRINKING WATER Buildings and Facilities § 129.35... is not considered water of a safe, sanitary quality as required for use in bottled water by paragraph... comply with bottled water quality standards (§ 165.110(b) of this chapter) and section 402(a)(1) and (a...

Methodology for the design of Santa Rosa de Cabal sanitary landfill, Risaralda

International Nuclear Information System (INIS)

Tabares, J; Orozco, J

1992-01-01

In 1987 the Regional Autonomous Corporation of Risaralda, CARDER and the Risaralda Government, they signed a cooperation agreement, in order to endowing from sanitary landfill to the municipalities of the department. In the mark of this agreement it was carried out the design of Santa Rosa's sanitary landfill, that with near 50.000 inhabitants it is constituted in the third city of the department. This city generates some 25 tons/day of garbage that at the present time are heady directly to the waters of San Eugenio River. The present work contains the most important methodological aspects in the design of the sanitary landfill and some comments about the approaches ideal Vs real approaches of selection of places

Trichuris suis secrete products that reduce disease severity in a multiple sclerosis model

DEFF Research Database (Denmark)

Hansen, Christine Soholm; Hasseldam, Henrik; Bacher, Idahella Hyldgaard

2017-01-01

, including parasites. Parasites are known to employ different immunomodulatory and antiinflammatory strategies, which enable them to evade destruction by the immune system. We have investigated the immunomodulation by the swine whipworm, Trichuris suis, by measuring the impact of oral administration of T...

Evaluation of sanitary impact of environmental pollution and quantitative evaluation of sanitary risks; Estimation de l'impact sanitaire d'une pollution environnementale et evaluation quantitative des risques sanitaires

Energy Technology Data Exchange (ETDEWEB)

NONE

2005-09-15

The calculation of a sanitary impact present a great interest at the decision level for the decision-makers and the whole of concerned actors. It constitutes a first step to organize a social debate around the risk acceptance, to analyze the feasibility of an inquiry or an epidemiological surveillance or to proportion an activity leading to pollutants emission in natural medium. Several conclusions are brought out: it is justified to estimate a sanitary impact from a sanitary risk excess, especially coming from animal tissue. It is conceivable to go beyond an estimation of the only individual risk and to calculate a number of cases in excess in the concerned population. The working group underlines that the characteristics of the situation are the determining factor to give the type of response to bring. The effective of the population is an important element and a situation has not to be underestimated because of the size at the pretext that the excess calculation leads to a number of cases inferior to one leading to believe that the impact is minor or negligible while the individual probability is high. The sanitary impact, expressed by the number of cancer cases in excess in an exposed population is quantified from the average value of excess of sanitary risk multiplied by the population effective, and expressed with a confidence interval. The sanitary impact can be expressed under the form of a percentage of the population present in the exposure area and goes past the comparison marks usually pointed up. This practice must be cheered. An analysis of uncertainties must be made as often as possible. (N.C.)

Immune responses and parasitological observations induced during probiotic treatment with medicinal Trichuris suis ova in a healthy volunteer

DEFF Research Database (Denmark)

Williams, Andrew R; Dige, Anders; Rasmussen, Tue Kruse

2017-01-01

Ingestion of eggs (ova) of the porcine nematode parasite Trichuris suis (TSO) may reduce the severity of autoimmune disorders, however the development of TSO treatment as a useful therapy for autoimmune diseases is hampered by a lack of knowledge on the development of the parasite and the nature...... was also reflected to some extent in the peripheral circulation. These results, together with the first definitive observations that T. suis can mature to adult size and reproduce in humans, shed new light on the interaction between the human immune system and probiotic helminth treatment, which should...

Marginal vitamin A deficiency in pigs experimentally infected with Trichuris suis

DEFF Research Database (Denmark)

Pedersen, S; Saeed, I; Jensen, S K

2001-01-01

were marginally smaller (36.7 vs 40.2 mm; P = 0.08), more orally located (section 2.9 vs 3.9; P = 0.08) and had a higher proportion of males (0.58 vs 0.50; P = 0.08) whereas there were no effects of diet treatment on fecundity. The proportion of pigs with faecal T. suis egg excretion 12 weeks post...

Sanitary landfill energetic potential analysis: a real case study

International Nuclear Information System (INIS)

Desideri, Umberto; Di Maria, Francesco; Leonardi, Daniela; Proietti, Stefania

2003-01-01

Waste disposal represents an important problem in developed countries. Many different techniques are available to reduce the amount of waste production and its environmental impact. In most cases, sanitary landfills have been and continue to be one of the most common ways to dispose of urban and industrial wastes. It is well known how landfilling produces an important environmental drawback due to gaseous, liquid and solid emissions that are dangerous for the environment. Landfill biogas emissions contain mainly carbon dioxide and methane. In particular, the methane concentration can be higher than 50% by volume. This means that the calorific value of sanitary landfill biogas can be higher than 18,000 kJ/N m 3 . The utilization of such gas as fuel for electrical and thermal energy production can be an important way to reduce the landfill impact on the environment and represent an easy way to use a renewable energy source. In the following, the amount and composition of the biogas produced in a sanitary landfill situated in central Italy have been analysed. Experimental results have been discussed, and an energetic potential evaluation has been performed

Sanitary landfill energetic potential analysis: a real case study

Energy Technology Data Exchange (ETDEWEB)

Desideri, Umberto E-mail: umberto.desideri@unipg.it; Di Maria, Francesco E-mail: fdm@unipg.it; Leonardi, Daniela; Proietti, Stefania

2003-07-01

Waste disposal represents an important problem in developed countries. Many different techniques are available to reduce the amount of waste production and its environmental impact. In most cases, sanitary landfills have been and continue to be one of the most common ways to dispose of urban and industrial wastes. It is well known how landfilling produces an important environmental drawback due to gaseous, liquid and solid emissions that are dangerous for the environment. Landfill biogas emissions contain mainly carbon dioxide and methane. In particular, the methane concentration can be higher than 50% by volume. This means that the calorific value of sanitary landfill biogas can be higher than 18,000 kJ/N m{sup 3}. The utilization of such gas as fuel for electrical and thermal energy production can be an important way to reduce the landfill impact on the environment and represent an easy way to use a renewable energy source. In the following, the amount and composition of the biogas produced in a sanitary landfill situated in central Italy have been analysed. Experimental results have been discussed, and an energetic potential evaluation has been performed.

The Surface-Exposed Protein SntA Contributes to Complement Evasion in Zoonotic Streptococcus suis.

Science.gov (United States)

Deng, Simin; Xu, Tong; Fang, Qiong; Yu, Lei; Zhu, Jiaqi; Chen, Long; Liu, Jiahui; Zhou, Rui

2018-01-01

Streptococcus suis is an emerging zoonotic pathogen causing streptococcal toxic shock like syndrome (STSLS), meningitis, septicemia, and even sudden death in human and pigs. Serious septicemia indicates this bacterium can evade the host complement surveillance. In our previous study, a functionally unknown protein SntA of S. suis has been identified as a heme-binding protein, and contributes to virulence in pigs. SntA can interact with the host antioxidant protein AOP2 and consequently inhibit its antioxidant activity. In the present study, SntA is identified as a cell wall anchored protein that functions as an important player in S. suis complement evasion. The C3 deposition and membrane attack complex (MAC) formation on the surface of sntA -deleted mutant strain Δ sntA are demonstrated to be significantly higher than the parental strain SC-19 and the complementary strain CΔ sntA . The abilities of anti-phagocytosis, survival in blood, and in vivo colonization of Δ sntA are obviously reduced. SntA can interact with C1q and inhibit hemolytic activity via the classical pathway. Complement activation assays reveal that SntA can also directly activate classical and lectin pathways, resulting in complement consumption. These two complement evasion strategies may be crucial for the pathogenesis of this zoonotic pathogen. Concerning that SntA is a bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase in many species of Gram-positive bacteria, these complement evasion strategies may have common biological significance.

Susceptibility to antimicrobial agents of Streptococcus suis capsular type 2 strains isolated from pigs.

Science.gov (United States)

Seol, B; Kelneric, Z; Hajsig, D; Madic, J; Naglic, T

1996-03-01

The minimal inhibitory concentrations (MICs) for thirty-three epidemiologicaly unrelated clinical isolates of Streptococcus suis capsular type 2 were determined in relation to ampicillin, ampicillin-sulbactam, amoxicillin, clavulanate-amoxicillin, penicillin G, cephalexin, gentamicin, streptomycin, erythromycin, tylosin and doxycycline, using the microtitre broth dilution procedure described by the U.S. National Committee for Clinical Laboratory Standards (NCCLS). Gentamicin was the most active compound tested, with an MIC for 90% of the strains tested (MIC(90)) of 0.4 mg/L. Overall, 70% of strains were resistant to doxycycline (MIC(90) > or = 100.0 mg/L), followed by penicillin G (51% of strains) (MIC(90) + or = 100.0 mg/L). Resistance to amoxicillin and ampicillin was 36.4% (MIC(90) 12.5 mg/L) and 33.3% (MIC(90) 50.0 mg/L), respectively. 15.2% of S. suis strains were resistant to streptomycin, tylosin and cephalexin with MIC90 values of 25.0 mg/L, 12.5 mg/L and 25.0 mg/L, respectively. A combination of ampicillin and sulbactam (MIC(90) 6.3 mg/L) and a combination of amoxicillin and clavulanate (MIC(90) 3.1 mg/L) as well as erythromycin (1.6 mg/L) were of the same efficacy, with a total of 9.1% resistant S. suis strains. This high percentage of resistance to doxycycline and penicillin G precludes the use of these antibiotics as empiric therapy of swine diseases.

Glucose 6-phosphate dehydrogenase: isoenzymatic pattern in Oesophagostomum venulosum, Trichuris ovis and T. suis.

Science.gov (United States)

Rodriguez, B; Cutillas, C; German, P; Guevara, D

1991-12-01

In the present communication we have studied the isoenzymatic pattern activity of the glucose 6-phosphate dehydrogenase (G6PD) in Oesophagostomum venulosum, Trichuris ovis and T. suis, parasites of Capra hircus (goat), Ovis aries (sheep) and Sus scrofa domestica (pig) respectively, by polyacrylamide gel electrophoresis. Different phenotypes have been observed in the G6PD isoenzymatic pattern activity in males and females of Oesophagostomum venulosum. Furthermore, G6PD activity has been assayed in Trichuris ovis collected from Ovis aries and Capra hircus. No differences have been observed in the isoenzymatic patterns attending to the different hosts. All the individuals exhibited one single band or two bands; this suggests a monomeric condition for G6PD in T. ovis. In T. suis the enzyme G6PD appeared as a single electrophoretic band in about 85.7% of the individuals.

Detección de Mycoplasma suis en casos clínicos de síndrome del desmedro multisistémico posdestete en porcinos Detection of Mycoplasma suis in clinical cases with postweaning multisystemic wasting syndrome in swine

Directory of Open Access Journals (Sweden)

N. B. Pereyra

2006-09-01

Full Text Available Mycoplasma suis es un parásito obligado de los eritrocitos del cerdo. Produce anemia aguda o crónica y afecta a distintas categorías de animales. El síndrome del desmedro multisistémico posdestete (PMWS se considera causado por el circovirus porcino tipo 2 (PCV-2, aunque muchos aspectos de la patogenia del síndrome permanecen sin aclarar. Se manifiesta a través de retraso del crecimiento, anemia e ictericia en cerdos de 5 a 12 semanas de edad, en los que se produce una inmunosupresión que deriva en coinfecciones bacterianas. Se estudiaron tres granjas porcinas con sintomatología asociada a la presencia de estos dos agentes etiológicos. Se observaron formas compatibles con M. suis en extendidos de sangre y en lesiones histopatológicas indicativas de PMWS, en cortes de tejidos. Esta es la primera comunicación acerca de la asociación clínica entre las dos entidades mencionadas.Mycoplasma suis is a swine erythrocyte obligatory parasite. Its presence may result in chronic or acute anaemia in different pig categories. It is considered that the postweaning multisystemic wasting syndrome (PMWS is caused by porcine circovirus type 2, but some aspects of the pathogenesis remain unknown. PMWS signs are impaired weight gain, anaemia and jaundice in 5 to 12 week-old pigs that suffer from immunosuppression and bacterial co-infections. The pigs with signs of these diseases on three porcine farms were studied. Compatible M. suis forms in blood smears and typical PMWS lesions in tissue cuts were seen. This is the first communication of the clinical association between these two entities.

Efficacy of Antimicrobial Treatments and Vaccination Regimens for Control of Porcine Reproductive and Respiratory Syndrome Virus and Streptococcus suis Coinfection of Nursery Pigs

Science.gov (United States)

Halbur, P.; Thanawongnuwech, R.; Brown, G.; Kinyon, J.; Roth, J.; Thacker, E.; Thacker, B.

2000-01-01

Seventy-six, crossbred, porcine reproductive and respiratory syndrome virus (PRRSV)-free pigs were weaned at 12 days of age and randomly assigned to seven groups of 10 to 11 pigs each. Pigs in group 1 served as unchallenged controls. Pigs in groups 2 to 7 were challenged intranasally with 2 ml of high-virulence PRRSV isolate VR-2385 (104.47 50% tissue culture infective doses per 2 ml) on day 0 of the study (30 days of age). Seven days after PRRSV challenge, pigs in groups 2 to 7 were challenged intranasally with 2 ml of Streptococcus suis serotype 2 (108.30 CFU/2 ml). Group 2 pigs served as untreated positive controls. Antimicrobial treatments included daily intramuscular injection with 66,000 IU of procaine penicillin G per kg of body weight on days 8 to 10 (group 3), drinking water medication with 23.1 mg of tiamulin per kg during days 8 to 10 (group 4), and daily intramuscular injection of 5.0 mg of ceftiofur hydrochloride per kg on days 8 to 10 (group 5). Vaccination regimens included two intramuscular doses of an autogenous killed S. suis vaccine (group 6) prior to S. suis challenge or a single 2-ml intramuscular dose of an attenuated live PRRSV vaccine (group 7) 2 weeks prior to PRRSV challenge. Mortality was 0, 63, 45, 54, 9, 40, and 81% in groups 1 to 7, respectively. Ceftiofur treatment was the only regimen that significantly (P < 0.05) reduced mortality associated with PRRSV and S. suis coinfection. The other treatments and vaccinations were less effective. We conclude that ceftiofur administered by injection for three consecutive days following S. suis challenge was the most effective regimen for minimizing disease associated with PRRSV and S. suis coinfection. PMID:10699012

Control of Competence for DNA Transformation in Streptococcus suis by Genetically Transferable Pherotypes

NARCIS (Netherlands)

Zaccaria, E.; Baarlen, van P.; Greeff, de A.; Morrison, D.A.; Smith, H.; Wells, J.M.

2014-01-01

Here we show that S. suis, a major bacterial pathogen of pigs and emerging pathogen in humans responds to a peptide pheromone by developing competence for DNA transformation. This species does not fall within any of the phylogenetic clusters of streptococci previously shown to regulate competence

Attività solare, effetti ionosferici e servizi NRTK: quali connessioni?

Directory of Open Access Journals (Sweden)

Francesco Mancini

2012-06-01

Full Text Available Come noto anche dalle più recenti cronache dei quotidiani l’attività solare si trova in un periodo di rapida crescita, all’interno di un ciclo periodico della durata di 11 anni. Questo si traduce nella presenza di macchie solari (Sunspot, eruzioni solari ed emissione di massa coronarica che producono campi magnetici che dalla superficie, attraverso il cosiddetto vento solare, arrivano ad alterare l’equilibrio del campo magnetico terrestre. Lo strato più periferico dell’atmosfera terrestre, la ionosfera, è il primo a subire gli effetti prodotti dall’attività solare in quanto gli ioni e le molecole ionizzate che esso continente tendono ad aumentare come numero in seguito all’attività ionizzante prodotta dal “vento solare”. La ionizzazione dello strato ionosferico, a sua volta, incide sulla propagazione dei segnali GNSS, essendo la ionosfera un mezzo dispersivo (che influenza in modo selettivo i segnali in funzione delle loro caratteristiche. Questo studio cerca di verificare quali possono essere gli effetti dell’attività solare sulle prestazioni dei servizi NRTK (Network Real Time Kinematic attraverso l’analisi delle prestazioni raggiunte dagli utenti di un servizio di posizionamento nell’anno 2011, periodo caratterizzato da una rapida ascesa dell’attività solare verso i massimi previsti per il primo semestre del 2013. As recognized by hundreds of years of sun observation by the researcher in eliophysics, the Sun is now entering in a period of increased activity. The producing of sunspots, solar flares and coronal mass ejection from the Sun’s surface is able to create anomalies in the magnetic field that could stream towards the Earth. The terrestrial magnetic field may be influenced by the “solar wind” and the ionized upper layer of the atmosphere, called ionosphere, could be affected by scintillation and induced delays of the travelling signals. Several critical infrastructures would be influenced by such a

Development of Ukrainian legislation on sanitary protection of water resources in the XX century

Directory of Open Access Journals (Sweden)

Ю. А. Чуприна

2015-05-01

Full Text Available Problem Setting. The article investigates the formation in Ukraine of legal regulation of the state sanitary control of water, the process of creating of general and special jurisdiction for its implementation, as well as the regulation regime, methods and tools for health protection of waters of different categories. The relevance of this article due to the need to systematize the individual areas of water law, including the protection of water and sanitation of the twentieth century. Analysis of the recent researches and publications. The article devoted to the study of formation in Ukraine of legal regulation of the state sanitary control of water, the process of creating of general and special jurisdiction for its implementation, as well as the regulation regime, methods and tools for health protection of waters of different categories. Paper objective. The relevance of this article due to the need to identify viable methods and instruments of legal regulation of sanitary protection of water during the formation of the main areas of water legislation of Ukraine in the twentieth century. Terms used in the legal protection of water, sanitary water use rules, the bodies of sanitary supervision, the state sanitary inspection. Conclusions. Research in the field of water legislation, and therefore the individual concerned and its protection issues, many scientists were engaged as ecologists and environmentalists. However, the special historical and legal work in a range twentieth century. no. This gap makes it difficult to analyze the current state of affairs in the field of sanitary protection of water. Detection of historical stages of development of water legislation in this area, identifying the main patterns and dynamics of legal regulation of sanitary protection of water in specific historical circumstances will improve the current regime of water protection. The author analyzes the main laws and regulations in the field of materials of

On professional and official requirements to physicians in radiation health by sectoral sanitary and epidemiological stations

International Nuclear Information System (INIS)

Usol'tsev, V.I.; Konkina, L.F.; Shishenina, V.I.

1989-01-01

Professional and official requirements (POR) to sanitary physician, which deals with radiation hygiene at the sanitary and epidemiologic stations (SES), are considered. These requirements determine minimum of professional skills and abilities in the field of radiation hygiene. Physician should contribute to the improvement of radiation safety and health indices for personnel and population, and in this case, his activity should not impede the further usage of ionizing radiation sources in the national economy. Sanitary physician, dealing with a actain branch of industry, concerning the problems of radiation hygiene should know the principles of deontology, aims and functions of SES establishment and departments in the field of radiation hygiene, legal principles of radiation safety is basic tasks are as follows: 1) State sanitary inspection of sanitary-hygienic measures for the environmental protection and radiation protection of population; 2) organizational and methodological activity; 3) activity in medical civil defense

The transcriptome of Trichuris suis--first molecular insights into a parasite with curative properties for key immune diseases of humans.

Directory of Open Access Journals (Sweden)

Cinzia Cantacessi

Full Text Available Iatrogenic infection of humans with Trichuris suis (a parasitic nematode of swine is being evaluated or promoted as a biological, curative treatment of immune diseases, such as inflammatory bowel disease (IBD and ulcerative colitis, in humans. Although it is understood that short-term T. suis infection in people with such diseases usually induces a modified Th2-immune response, nothing is known about the molecules in the parasite that induce this response.As a first step toward filling the gaps in our knowledge of the molecular biology of T. suis, we characterised the transcriptome of the adult stage of this nematode employing next-generation sequencing and bioinformatic techniques. A total of ∼65,000,000 reads were generated and assembled into ∼20,000 contiguous sequences ( = contigs; ∼17,000 peptides were predicted and classified based on homology searches, protein motifs and gene ontology and biological pathway mapping.These analyses provided interesting insights into a number of molecular groups, particularly predicted excreted/secreted molecules (n = 1,288, likely to be involved in the parasite-host interactions, and also various molecules (n = 120 linked to chemokine, T-cell receptor and TGF-β signalling as well as leukocyte transendothelial migration and natural killer cell-mediated cytotoxicity, which are likely to be immuno-regulatory or -modulatory in the infected host. This information provides a conceptual framework within which to test the immunobiological basis for the curative effect of T. suis infection in humans against some immune diseases. Importantly, the T. suis transcriptome characterised herein provides a curated resource for detailed studies of the immuno-molecular biology of this parasite, and will underpin future genomic and proteomic explorations.

[Sanitary-hygienic assessment of microbial biofertilizer].

Science.gov (United States)

Arkhipchenko, N A; Akhtemava, G A; Lebedeva, T V; Voronina, A A; Makhan'kova, T I; Pavlova, M M; Shteĭntsaĭg, T A

1991-10-01

Biological treatment of sewage from pig-breeding complexes allowed to produce microbial biomass and primary sediments. The mixture of these components (1:1) after rendering harmless and drying out become the high effective biofertilizer. The results of chronic experiment on sanitary status of soil (microbial and helminthological indexes) under this biofertilizer usage are discussed, and the harmlessness of it is demonstrated.

Draft genome sequences of nine Streptococcus suis strains isolated in the United States

Science.gov (United States)

Streptococcus suis is a swine pathogen responsible for economic losses to the pig industry worldwide. Additionally, it is a zoonotic agent that can cause severe infections in those in close contact with infected pigs and/or who consume uncooked or undercooked pork products. Here, we report nine draf...

Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari Uso de um protocolo de PCR para a detecção de Mycoplasma suis para avaliação de uma população de catetos e queixadas de cativeiro (Tayassu tajacu and Tayassu pecari

Directory of Open Access Journals (Sweden)

Rafael Felipe da Costa Vieira

2011-03-01

Full Text Available Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species.Mycoplasma suis é uma bactéria hemotrópica dos eritrócitos e é o agente causador da eperitrozoonose suína. O diagnóstico da infecção pode ser realizado pelo exame direto de esfregaços sanguíneos; entretanto, o uso da reação em cadeia da polimerase (PCR baseada no gene 16S RNA de M. suis aumenta a sensibilidade e especificidade da detecção. O objetivo deste estudo foi avaliar catetos e queixadas (Tayassu tajacu e T. pecari para a infecção por M. suis, utilizando PCR convencional específico. Um total de 28 amostras de sangue de catetos e queixadas de cativeiro foram coletadas, o DNA foi extraído e a PCR específica para a detecção de M. suis realizada. Todas as amostras foram negativas pelo esfregaço sanguíneo e PCR. Para verificar a presença de DNA amplificável, PCR para o gene da beta actina foi realizada em todas as amostras. Este estudo foi parte de um programa de vigilância ativa, o qual é crucial para o monitoramento do estado de saúde animal, particularmente em espécies selvagens.

Construction, characterization and evaluation of the protective efficacy of the Streptococcus suis double mutant strain ΔSsPep/ΔSsPspC as a live vaccine candidate in mice.

Science.gov (United States)

Hu, Jin; You, Wujin; Wang, Bin; Hu, Xueying; Tan, Chen; Liu, Jinlin; Chen, Huanchun; Bei, Weicheng

2015-01-01

Streptococcus suis serotype 2 (S. suis 2) causes sepsis and meningitis in piglets and humans, and results in one of the most serious bacterial diseases affecting the production of commercial pigs around the world. Due to the failure of the current inactivated vaccine to protect against the disease, development of a new attenuated live vaccine against S. suis 2 by deleting essential virulence factors is urgently needed. We have previously reported the construction and characterization of an SsPep single gene deletion mutant strain ΔSsPep based on S. suis 2. Our previous results have shown that SsPep plays a critical role in the pathogenesis of S. suis 2. In this study, a precisely defined double-deletion mutant ΔSsPep/ΔSsPspC of S. suis 2 without antibiotic-resistance markers was constructed based on ΔSsPep, and the levels of virulence of the wild-type (WT) and ΔSsPep/ΔSsPspC were compared in a mouse experimental infection model. We demonstrated that the double mutant ΔSsPep/ΔSsPspC was less virulent than the WT, and could induce a noticeable antibody response. Analysis of IgG subclasses (IgG1 and IgG2a) indicated that both Th1 and Th2 responses were induced by ΔSsPep/ΔSsPspC, although the IgG2a (Th1) response predominated over the IgG1 (Th2) response. Moreover, ΔSsPep/ΔSsPspC could confer 90% protective efficacy against challenge with a lethal dose of fully virulent S. suis 2. Taken together, these data demonstrate that ΔSsPep/ΔSsPspC can be used as an effective live vaccine and provide a novel strategy against infection of S. suis 2. Copyright © 2014 Elsevier GmbH. All rights reserved.

Salmonella enterica serovar Choleraesuis vector delivering SaoA antigen confers protection against Streptococcus suis serotypes 2 and 7 in mice and pigs.

Science.gov (United States)

Li, Yu-An; Ji, Zhenying; Wang, Xiaobo; Wang, Shifeng; Shi, Huoying

2017-12-21

Streptococcus suis is one of the major pathogens that cause economic losses in the swine industry worldwide. However, current bacterins only provide limited prophylactic protection in the field. An ideal vaccine against S. suis should protect pigs against the clinical diseases caused by multiple serotypes, or at least protect against the dominant serotype in a given geographic region. A new recombinant Salmonella enterica serotype Choleraesuis vaccine vector, rSC0011, that is based on the regulated delayed attenuation system and regulated delayed antigen synthesis system, was developed recently. In this study, an improved recombinant attenuated Salmonella Choleraesuis vector, rSC0016, was developed by incorporating a sopB mutation to ensure adequate safety and maximal immunogenicity. In the spleens of mice, rSC0016 colonized less than rSC0011. rSC0016 and rSC0011 colonized similarly in Peyer's patches of mice. The recombinant vaccine rSC0016(pS-SaoA) induced stronger cellular, humoral, and mucosal immune responses in mice and swine against SaoA, a conserved surface protein that is present in many S. suis serotypes, than did rSC0011(pS-SaoA) without sopB or rSC0018(pS-SaoA), which is an avirulent, chemically attenuated vaccine strain. rSC0016(pS-SaoA) provided 100% protection against S. suis serotype 2 in mice and pigs, and full cross-protection against SS7 in pigs. This new vaccine vector provides a foundation for the development of a universal vaccine against multiple serotypes of S. suis in pigs.

Sorotipagem de amostras de Streptococcus suis isoladas de suínos em granjas dos Estados de São Paulo, Minas Gerais e Paraná Serotyping of Streptococcus suis strains isolated from pigs in the States of São Paulo, Minas Gerais e Paraná, Brazil

Directory of Open Access Journals (Sweden)

Keila J.R. Pagnani

2002-01-01

Full Text Available Infecções causadas por Streptococcus suis são muito comuns em países onde a indústria de carne suína é desenvolvida. Estas infecções estão relacionadas a casos clínicos de broncopneumonia, meningite, artrite, pericardite, miocardite, endocardite, poliserosite fibrinosa, septicemia, rinite e aborto. Esta bactéria também foi descrita como patógeno de ruminantes e humanos. No Brasil há evidências clínicas da existência de processos infecciosos causados por S. suis afetando mais de 50% das granjas em Estados como São Paulo, Minas Gerais e Paraná. No presente estudo foram isoladas 51 amostras de S. suis de granjas do Estados acima referidos, coletadas de diferentes casos clínicos como septicemia, meningite, artrite e pneumonia, tendo sido obtidas ou em cultura pura ou como patógeno de maior predominância nos tecidos de suínos. Este material foi semeado em Columbia ágar sangue adicionado de 5% de sangue bovino e incubado a 37°C por 24 horas. Para a identificação bioquímica as colônias que apresentavam a-hemólise, bem como as amostras padrão, foram submetidas a testes convencionais para a confirmação da espécie S. suis, tais como: hidrólise de arginina, teste de Voges-Proskauer, e produção de ácido a partir de vários carboidratos (inulina, salicina, trealose, lactose, sacarose, sorbitol, manitol e glicerol. As amostras também foram testadas para habilidade de crescimento em meio de TSA com 6,5% de NaCl e para a produção de amilase. Todas as amostras que fizeram parte desta pesquisa foram testadas pelo sistema Api 20 Strep para confirmação dos resultados obtidos nos testes convencionais. Para a sorotipagem foram produzidos antissoros de 1 a 8. Outras amostras não pertencentes a estes sorotipos também foram sorotipadas. O antissoro produzido em coelhos foi titulado pelo teste de aglutinação em tubo com 2-mercaptoetanol e pelo teste de reação capsular e, quando adequados, foram usados no teste de co

Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine

Science.gov (United States)

Calzas, Cynthia; Shiao, Tze Chieh; Neubauer, Axel; Kempker, Jennifer; Roy, René; Gottschalk, Marcelo

2016-01-01

Streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was selected to elicit opsonizing anti-capsular polysaccharide (anti-CPS) IgG antibodies. In this work, glycoconjugate prototypes were prepared by coupling S. suis type 2 CPS to tetanus toxoid, and the immunological features of the postconjugation preparations were evaluated in vivo. In mice, experiments evaluating three different adjuvants showed that CpG oligodeoxyribonucleotide (ODN) induces very low levels of anti-CPS IgM antibodies, while the emulsifying adjuvants Stimune and TiterMax Gold both induced high levels of IgGs and IgM. Dose-response trials comparing free CPS with the conjugate vaccine showed that free CPS is nonimmunogenic independently of the dose used, while 25 μg of the conjugate preparation was optimal in inducing high levels of anti-CPS IgGs postboost. With an opsonophagocytosis assay using murine whole blood, sera from immunized mice showed functional activity. Finally, the conjugate vaccine showed immunogenicity and induced protection in a swine challenge model. When conjugated and administered with emulsifying adjuvants, S. suis type 2 CPS is able to induce potent IgM and isotype-switched IgGs in mice and pigs, yielding functional activity in vitro and protection against a lethal challenge in vivo, all features of a T cell-dependent response. This study represents a proof of concept for the potential of glycoconjugate vaccines in veterinary medicine applications against invasive bacterial infections. PMID:27113360

[Street food: analysis of hygienic and sanitary conditions of food handlers].

Science.gov (United States)

de Souza, Giovanna Carbonera; dos Santos, Celso Tadeu Barbosa; Andrade, Anderson Assunção; Alves, Luciene

2015-08-01

The consumption of street food is an economical and practical alternative for the population. However, concerns regarding the safety of these foods are increasing. Thus, this study aimed to analyze the hygienic and sanitary conditions of the street food trade in Uberaba in the State of Minas Gerais. For this purpose, 30 street vending sites were evaluated using a structured questionnaire and microbiological analysis of food handlers' hands and food contact recipients and surfaces. The findings showed low adequacy of street food regarding hand hygiene and food contact surfaces. With respect to surfaces, the results for mesophilic aerobic microorganisms revealed that only 23.3% had satisfactory sanitary conditions. The level of contamination of hands by fecal thermotolerant coliforms and/or Coagulase positive Staphylococci was unsatisfactory in approximately 47% of food handlers. The conclusion drawn is that the hygienic and sanitary conditions of food handlers in Uberaba are poor. It is therefore necessary to adopt effective and permanent training programs on food safety for food handlers urgently.

Pollution loads in urban runoff and sanitary wastewater.

Science.gov (United States)

Taebi, Amir; Droste, Ronald L

2004-07-05

While more attention has been paid in recent years to urban point source pollution control through the establishment of wastewater treatment plants in many developing countries, no considerable planning nor any serious measures have been taken to control urban non-point source pollution (urban stormwater runoff). The present study is a screening analysis to investigate the pollution loads in urban runoff compared to point source loads as a first prerequisite for planning and management of receiving water quality. To compare pollutant loads from point and non-point urban sources, the pollutant load is expressed as the weight of pollutant per hectare area per year (kg/ha.year). Unit loads were estimated in stormwater runoff, raw sanitary wastewater and secondary treatment effluents in Isfahan, Iran. Results indicate that the annual pollution load in urban runoff is lower than the annual pollution load in sanitary wastewater in areas with low precipitation but it is higher in areas with high precipitation. Two options, namely, advanced treatment (in lieu of secondary treatment) of sanitary wastewater and urban runoff quality control systems (such as detention ponds) were investigated as controlling systems for pollution discharges into receiving waters. The results revealed that for Isfahan, as a low precipitation urban area, advanced treatment is a more suitable option, but for high precipitation urban areas, urban surface runoff quality control installations were more effective for suspended solids and oxygen-demanding matter controls, and that advanced treatment is the more effective option for nutrient control.

Pollution loads in urban runoff and sanitary wastewater

International Nuclear Information System (INIS)

Taebi, Amir; Droste, Ronald L.

2004-01-01

While more attention has been paid in recent years to urban point source pollution control through the establishment of wastewater treatment plants in many developing countries, no considerable planning nor any serious measures have been taken to control urban non-point source pollution (urban stormwater runoff). The present study is a screening analysis to investigate the pollution loads in urban runoff compared to point source loads as a first prerequisite for planning and management of receiving water quality. To compare pollutant loads from point and non-point urban sources, the pollutant load is expressed as the weight of pollutant per hectare area per year (kg/ha·year). Unit loads were estimated in stormwater runoff, raw sanitary wastewater and secondary treatment effluents in Isfahan, Iran. Results indicate that the annual pollution load in urban runoff is lower than the annual pollution load in sanitary wastewater in areas with low precipitation but it is higher in areas with high precipitation. Two options, namely, advanced treatment (in lieu of secondary treatment) of sanitary wastewater and urban runoff quality control systems (such as detention ponds) were investigated as controlling systems for pollution discharges into receiving waters. The results revealed that for Isfahan, as a low precipitation urban area, advanced treatment is a more suitable option, but for high precipitation urban areas, urban surface runoff quality control installations were more effective for suspended solids and oxygen-demanding matter controls, and that advanced treatment is the more effective option for nutrient control

Brucella suis strain 2 vaccine is safe and protective against heterologous Brucella spp. infections.

Science.gov (United States)

Zhu, Liangquan; Feng, Yu; Zhang, Ge; Jiang, Hui; Zhang, Zhen; Wang, Nan; Ding, Jiabo; Suo, Xun

2016-01-12

Brucellosis is a wide spread zoonotic disease that causes abortion and infertility in mammals and leads to debilitating, febrile illness in humans. Brucella abortus, Brucella melitensis and Brucella suis are the major pathogenic species to humans. Vaccination with live attenuated B. suis strain 2 (S2) vaccine is an essential and critical component in the control of brucellosis in China. The S2 vaccine is very effective in preventing brucellosis in goats, sheep, cattle and swine. However, there are still debates outside of China whether the S2 vaccine is able to provide protection against heterologous virulent Brucella species. We investigated the residual virulence, immunogenicity and protective efficacy of the S2 vaccine in BALB/c mice by determining bacteria persistence in spleen, serum antibody response, cellular immune response and protection against a heterologous virulent challenge. The S2 vaccine was of low virulence as there were no bacteria recovered in spleen four weeks post vaccination. The vaccinated mice developed Brucella-specific IgG in 2-3 weeks, and a burst production of IFN-γ at one week as well as a two-fold increase in TNF-α production. The S2 vaccine protected mice from a virulent challenge by B. melitensis M28, B. abortus 2308 and B. suis S1330, and the S2 vaccinated mice did not develop any clinical signs or tissue damage. Our study demonstrated that the S2 vaccine is of low virulence, stimulates good humoral and cellular immunity and protects animals against infection by heterologous, virulent Brucella species. Copyright © 2015 Elsevier Ltd. All rights reserved.

DATABASES AND THE SUI-GENERIS RIGHT – PROTECTION OUTSIDE THE ORIGINALITY. THE DISREGARD OF THE PUBLIC DOMAIN

Directory of Open Access Journals (Sweden)

Monica LUPAȘCU

2018-05-01

Full Text Available This study focuses on databases as they are regulated by Directive no.96/9/EC regarding the protection of databases. There are also several references to Romanian Law no.8/1996 on copyright and neighbouring rights which implements the mentioned European Directive. The study analyses certain effects that the sui-generis protection has on public domain. The study tries to demonstrate that the reglementation specific to databases neglects the interests correlated with the public domain. The effect of such a regulation is the abusive creation of some databases in which the public domain (meaning information not protected by copyright such as news, ideas, procedures, methods, systems, processes, concepts, principles, discoveries ends up being encapsulated and made available only to some private interests, the access to public domain being regulated indirectly. The study begins by explaining the sui- generis right and its origin. The first mention of databases can be found in “Green Paper on Copyright (1998,” a document that clearly shows, the database protection was thought to cover a sphere of information non-protectable from the scientific and industrial fields. Several arguments are made by the author, most of them based on the report of the Public Consultation sustained in 2014 in regards to the necessity of the sui-generis right. There are some references made to a specific case law, namely British Houseracing Board vs William Hill and Fixture Marketing Ldt. The ECJ’s decision în that case is of great importance for the support of public interest to access information corresponding to some restrictive fields that are derived as a result of the maker’s activities, because in the absence of the sui-generis right, all this information can be freely accessed and used.

The sanitary officer: first aid coordinator on EDF nuclear power plant

International Nuclear Information System (INIS)

Masson, A.

2000-01-01

The internal organisation for first aid to the injured in case of an accident on E.D.F. nuclear power plant calls for the immediate assistance of a first aid team, consisting of five workers, under the direction of a principal first aid officer; one of the first aid workers, the sanitary officer who instructs the first aid workers intervention awaiting the arrival of an external medical. When the 'Sanitary on-site Emergency Plan' was up' dated, twenty medical doctors and seventy members of staff from five different sites were questioned as to the function of the sanitary officer. The conclusions revealed a notable difference of training amongst the different sites, and concerning first aid organisation, difference of priority of actions, extent of their participation once the medical team arrives and their participation in case of decontamination treatment. The medical doctors and staff lay a particular stress on importance of defining on a national scale the limits of role and responsibilities of the sanitary officer and establish a more specific training in this field, consequently motivating commitment and professionalism involvement. There is a great difference between the training and coaching of the first aid assistance and fire protection teams. To conclude, we propose that the first aid officer be known as first aid coordinator and the qualification of 'Certificat de Formation aux Premiers Secours en Equipe' in compliance with the current legislation together with a specific nuclear module and they should undergo regular on-site drills. (author)

Food Safety and Sanitary Practices of Selected Hotels in Batangas Province, Philippines: Basis of Proposed Enhancement Measures

Directory of Open Access Journals (Sweden)

April M. Perez

2017-02-01

Full Text Available This study assessed the extent of food safety and sanitary practices of selected hotels in Batangas province as basis of proposed enhancement measures. The study utilized descriptive method to describe food safety and sanitary practices of selected hotels in Batangas province with a total of 8 hotels (256 respondents. Purposive sampling was used in the study. The questionnaires were designed using the provision of the Sanitation Code of the Philippines, validated and finalized to come up with legitimate results. The study showed that there were eight (8 hotel respondents classified as two, three, four star with considerable years of experience and adequate number of employees. The hotels demonstrated the food safety and sanitary practices always in the areas of restaurant, bar service, catering and banquet and room service. The significant pair-wise comparison for restaurant, bar service, catering and banquet and room service shows that 2 star hotels greatly differs. The researcher recommends that the management should maintain high standard of food safety and sanitary practices among its staff, upgrade the food safety and sanitary practices for food safety accreditation, continuous training of the hotel managers/employees on food safety and sanitary practices.

Clinical management of Brucella suis infection in dogs and implications for public health.

Science.gov (United States)

James, D R; Golovsky, G; Thornton, J M; Goodchild, L; Havlicek, M; Martin, P; Krockenberger, M B; Marriott, Dje; Ahuja, V; Malik, R; Mor, S M

2017-01-01

Brucellosis caused by Brucella suis is a notifiable disease that has recently emerged in dogs in New South Wales (NSW). Given the potential for zoonotic transmission, euthanasia of affected dogs is recommended, but this action is not mandatory. We report the clinical management of three dogs that underwent treatment at their owners' request. A 14-month-old spayed female crossbreed originally obtained from an urban animal shelter underwent extensive investigations in 2011-12 for lameness and back pain, culminating in decompressive laminectomy. Diagnosis of multifocal discospondylitis and spinal empyema was made, with B. suis cultured from surgical biopsy specimens. The dog responded to long-term treatment using rifampicin and doxycycline. A second case of B. suis infection was diagnosed in January 2016 in a 3-year-old crossbreed pig-hunting dog with unilateral testicular enlargement. Following serological diagnosis the dog was given preliminary therapy using rifampicin and doxycycline, the affected testis was resected and the patient given a further month of combination therapy. In March 2016 a 7-year-old crossbreed pig-hunting dog with brucellosis was handled similarly, although both testes were removed. Brucellosis should be considered in the differential diagnosis of back pain, discospondylitis, lameness, abortion, prostatic abscessation and testicular/epididymal enlargement in dogs, especially if there is exposure to feral pigs or consumption of uncooked feral pig meat. Euthanasia is the only guarantee of reducing the public health risk to zero. However, where treatment is desired by the owner, combination therapy using rifampicin and doxycycline appears to be effective, when combined with surgical resection of infected tissues. Further monitoring of dogs during and after treatment is required to document cure. © 2017 Australian Veterinary Association.

Trichuris suis and Oesophagostomum dentatum show different sensitivity and accumulation of fenbendazole, albendazole and levamisole in vitro

DEFF Research Database (Denmark)

Hansen, Tina Vicky Alstrup; Nejsum, Peter; Friis, Christian

2014-01-01

BACKGROUND: The single-dose benzimidazoles used against Trichuris trichiura infections in humans are not satisfactory. Likewise, the benzimidazole, fenbendazole, has varied efficacy against Trichuris suis whereas Oesophagostomum dentatum is highly sensitive to the drug. The reasons for low...... treatment efficacy of Trichuris spp. infections are not known. METHODOLOGY: We studied the effect of fenbendazole, albendazole and levamisole on the motility of T. suis and O. dentatum and measured concentrations of the parent drug compounds and metabolites of the benzimidazoles within worms in vitro....... The motility and concentrations of drug compounds within worms were compared between species and the maximum specific binding capacity (Bmax) of T. suis and O. dentatum towards the benzimidazoles was estimated. Comparisons of drug uptake in living and killed worms were made for both species. PRINCIPAL FINDINGS...

Phase and Micro-Structural Characterization of Sanitary-Ware Fired at Different Temperature

OpenAIRE

ATHER HASSAN; YASEEN IQBAL; SYED ZAFAR ILYAS

2017-01-01

The three main ingredients of sanitary-ware are clay, feldspar and quartz. This ware is being widely used and has therefore, attracted the attention of researchers from time to time. Consequently, it has been extensively investigated. The present study describes the phase and micro-structural analysis of sanitary-ware samples collected from local (Durr Ceramics Peshawar) industry. XRD (X-Ray Diffraction) of samples fired at 1100oC reveals the presence of ? ? ? ? ?-quartz and primary mullite o...

Clinical utility of the MMPI-2-RF SUI items and scale in a forensic inpatient setting: Association with interview self-report and future suicidal behaviors.

Science.gov (United States)

Glassmire, David M; Tarescavage, Anthony M; Burchett, Danielle; Martinez, Jennifer; Gomez, Anthony

2016-11-01

In this study, we examined whether the 5 Minnesota Multiphasic Personality Inventory-2-Restructured Form (MMPI-2-RF; Ben-Porath & Tellegen, 2008/2011) Suicidal/Death Ideation (SUI) items (93, 120, 164, 251, and 334) would provide incremental suicide-risk assessment information after accounting for information garnered from clinical interview questions. Among 229 forensic inpatients (146 men, 83 women) who were administered the MMPI-2-RF, 34.9% endorsed at least 1 SUI item. We found that patients who endorsed SUI items on the MMPI-2-RF concurrently denied conceptually related suicide-risk information during the clinical interview. For instance, 8% of the sample endorsed Item 93 (indicating recent suicidal ideation), yet denied current suicidal ideation upon interview. Conversely, only 2.2% of the sample endorsed current suicidal ideation during the interview, yet denied recent suicidal ideation on Item 93. The SUI scale, as well as the MMPI-2-RF Demoralization (RCd) and Low Positive Emotions (RC2) scales, correlated significantly and meaningfully with conceptually related suicide-risk information from the interview, including history of suicide attempts, history of suicidal ideation, current suicidal ideation, and months since last suicide attempt. We also found that the SUI scale added incremental variance (after accounting for information garnered from the interview and after accounting for scores on RCd and RC2) to predictions of future suicidal behavior within 1 year of testing. Relative risk ratios indicated that both SUI-item endorsement and the presence of interview-reported risk information significantly and meaningfully increased the risk of suicidal behavior in the year following testing, particularly when endorsement of suicidal ideation occurred for both methods of self-report. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

DESIGN MANUAL: SULFIDE CONTROL IN SANITARY SEWERAGE SYSTEMS

Science.gov (United States)

One characteristic by which sanitary sewage is known to the public is its potential for creating odor nuisances. Sometimes it is the odors escaping from sewer manholes that cause complaints; more commonly, the source is a wastewater treatment plant. Yet there are wastewater treat...

Immune and inflammatory responses in pigs infected with Trichuris suis and Oesophagostomum dentatum

DEFF Research Database (Denmark)

Andreasen, Annette; Petersen, Heidi Huus; Kringel, Helene

2015-01-01

effect on O. dentatum populations has been observed in co-infected pigs. Forty-eight helminth naïve pigs were allocated into 4 groups in a 2-factorial design. Two groups were trickle inoculated with either 10 T. suis eggs/kg/day (Group T) or 20 O. dentatum L3/kg/day (Group O). Group OT was infected...

[Interaction of the bodies and institutions of the Russian Inspectorate for the protection of consumer rights and human welfare on sanitary-and-epidemiological examinations and issuing sanitary-and-epidemiological opinions].

Science.gov (United States)

Safonkina, S G

2009-01-01

The paper describes problems in the organization of the interaction of the Russian Inspectorate for the Protection of Consumer Rights and Human Welfare in Moscow and the Center for Hygiene and Epidemiology in Moscow to perform sanitary-and-epidemiological examinations and to issue sanitary-and-epidemiological opinions. The goals of setting up a one-window service and measures required for its effective work are defined. Positive results of one-window activities are shown.

Sanitary sewer rehabilitation at Lawrence Livermore National Laboratory

Energy Technology Data Exchange (ETDEWEB)

Vellinger, R. J.; Burton, R.; Fritschy, B.

1995-04-01

The objectives of this paper are the following: to present LLNL`s collection system and innovative approach to sanitary sewer rehabilitation; share issues identified and lessons learned from over four (4) years of rehabilitation work; and discuss proposed system standards for ongoing maintenance and repair activities.

Rule concerning sanitary protection against ionizing radiations: novelties

International Nuclear Information System (INIS)

Bercedo, A.; Carmena, P.; Prieto, J. A.; Rubio, G.; Sollet, E.; Sustacha, D.

2002-01-01

Last July the a new legal Rule concerning Sanitary Protection against Ionising Radiation was published, as a transposition of the EU Directive about the Basic Norms related to the sanitary protection of workers and population against the risks resultant of the ionising radiation. The origin of this legislation goes back to the revision of the protection doctrine by the International Commission of Radiation Protection (ICRP) en the year 1990. El scope of the revised Rule is the regulation of the protection of population and workers against ionising radiation, the establishment of the national protection system with its exposition and dose limits and the correspondent penalty regime. It also modifies the maximum radiation dose limits and reinforces the application of the optimisation principle in the use of ionising radiation. In this article, the novelties introduced by the new Rule are commented in detail, ordered by the Titles I to IX in which the Rule is divided. (Author)

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2

International Nuclear Information System (INIS)

Cheng, Xia; Lu, Guangwen; Qi, Jianxun; Cheng, Hao; Gao, Feng; Wang, Jundong; Yan, Jinghua

2010-01-01

Crystals of SAICAR synthase from S. suis serotype 2 were obtained in the presence of 40 mM aspartic acid substrate; they belonged to space group P2 and diffracted to 2.8 Å resolution. Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR synthase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.8 Å resolution and belonged to space group P2, with unit-cell parameters a = 70.2, b = 52.2, c = 153.9 Å, β = 102.8°

About the tasks of the state sanitary-epidemiological service in public health protection and sanitary-epidemiological well-being of population and environment in case of radiation emergency situations

International Nuclear Information System (INIS)

Perminova, G.S.; Tuchkevich, E.A.

1995-01-01

The paper has stated the role of the State Sanitary-Epidemiological Service in the controlled radiation-emergency process on large, medium and small scale. The study reviews the phase character of radiation-emergency process, some typical features of their contents and the actions of the State Sanitary-Epidemiological Service, including non-radiation hygienic aspects; the characteristic features of supervision in case of small accidents are pointed out. Several scientific and practical problems on the raised subject which regarded to be urgent in the opinion of the authors are offered for consideration. 13 refs

SANITARY ASPECT OF NUTRITION CONTROL IN PUBLIC KITCHEN IN NIS

Directory of Open Access Journals (Sweden)

Suzana Milutinovic

2006-07-01

Full Text Available The aim of the paper was to evaluate sanitary condition of food preparation and distribution in the "Public kitchen" in Nis, as well as the meals’ quality in order to assess the health risks. The local inspection of the objects and microbiological analysis of swaps were done according to standard methods. Energetical values and biochemical structure of the meals were assessed by bromatological methods and energetical density was calculated. The study was being conducted from 2002 – 2005. The results show that sanitary conditions in the object were not adequate, because of a high percentage of incorrect samples (13.4% from the total number of samples. Energetical values of meals were usually adequate (1157 – 1564 kcal, 4837 – 6581 kJ, but constantly decreased. The chemical structure of the meals was adequate too, meaning that meals were very well- planed. The energetic density of meals was high (1,18 – 1,42 kcal/g. Our conclusion is that there are no alternatives to continuous health care education of the medical stuff and regular sanitary hygienic control, all with the aim to accomplish complete social function of the public kitchen and to protect the health of its consumers.

Frequency of anti hepatitis C virus antibodies amongst sanitary workers in a tertiary care hospital in Pakistan

International Nuclear Information System (INIS)

Khan, A.Z.; Razzaq, K.; Ansari, J.K.; Niazzi, S.K.

2011-01-01

Objective: To determine the frequency of anti Hepatitis C Virus antibodies in sanitary workers at Military Hospital Rawalpindi and to identify additional risk factors in them for hepatitis C infection. Cross sectional study Place and Duration of Study: Department of medicine, Military Hospital (M.H.), Rawalpindi, Pakistan over six months. Patients and Methods: All sanitary workers working at Military Hospital Rawalpindi were tested for anti HCV antibodies by third generation ELISA. Results: Six percent of the study population was found to be positive for anti HCV antibodies. Conclusion: The frequency of anti HCV antibodies is fairly high in sanitary workers, working in this tertiary care hospital studied. HCV infection is more frequent in those sanitary workers who have longer duration of service. (author)

Factors associated with sanitary conditions of food and drinking ...

African Journals Online (AJOL)

Introduction: Food borne illness has been a global challenge and it persisted as a ... an absence of sanitary facilities for waste management was major cause. ... inspection on the establishments to promote and ensure proper hygiene and ...

Analysis of biogas in sanitary landfill Caieiras

Directory of Open Access Journals (Sweden)

Giovano Candiani

2011-06-01

Full Text Available In this work, the biogas in the Sanitary Landfill Caieiras is qualitatively evaluated, emphasizing the influence of the geomembrana and cover system of vertical drains in the vicinity to capture the landfill. It was possible to detect an increase in the percentage of methane and oxygen reduction, aiming at the commercialization of carbon credits and electricity production.

Indoor Environmental Conditions and Sanitary Practices in Selected ...

African Journals Online (AJOL)

Rapidly urbanizing cities are witnessing an increase in Day care centres (DCCs) whose environmental conditions are substandard. This scenario has negative consequences on the health of the DCC attendees and yet information on some of the indicators such as the level of sanitary practices is not adequately ...

Aspects regarding the hygienic-sanitary conditions at the level of certain dental medicine cabinets in Iasi County.

Science.gov (United States)

Cernei, E R; Maxim, Dana Cristiana; Indrei, L L

2013-01-01

This baseline study aims to find out the evaluation of hygienic-sanitary conditions at the level of dental medicine cabinets through the verification of certain hygienic aspects. The study conducted consists in monitoring the hygienic/sanitary conditions at the level of 68 dental medicine cabinets (40 private cabinets and 28 school/university dental cabinets in Iasi county), using sheets for the assessment of the hygienic/sanitary conditions adapted from the control sheets of existing dental medicine cabinets at the level of DSP (Public Health Department) Iasi. The sheets for the assessment of the hygienic/sanitary conditions were evaluated by a specialized team and the results were i llustrated in the specific charts. At the level of all the dental cabinets the study revealed nonconformities regarding the means to carry out cleaning, disinfection operations, including the management of perilous waste, the control of medical personnel. An optimization of the hygienic-sanitary conditions at the level of dental medicine cabinets is still necessary, through participation to the activity of personnel training, who is directly involved in dental medical assistance.

The application of irradiation to phyto sanitary problems

Energy Technology Data Exchange (ETDEWEB)

Ross, R T [USDA/APHIS/PPQ. Department of Agriculture, Room 1630 Soagribg, 1400 Independence Ave. Sw. Mail Code Stop 3438, 20250 Washington D.C. (United States)

1998-12-31

The first formally adopted regulatory policy for irradiation as a phyto sanitary treatment in the United States was issued in 1989 and was based on Title 7 of the Code of Federal Regulations. These regulations authorized irradiation as a quarantine treatment for papayas intended for movement from the State of Hawaii to the continental United States (U.S.), Guam, Puerto Rico, and the U.S. Virgin Islands. This authorization was specific for commodity, place of origin, and program, but was designed for a complex of three fruit flies rather than a single pest. Routine commercial shipments were never realized under this regulation due to the lack of a treatment facility in Hawaii. However, the authorization has proven useful from the standpoint of beginning to establish policies for irradiation as a phyto sanitary treatment in the United States. The United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ) remains dedicated to using the most up-to-date, appropriate and least intrusive technology to provide quarantine security. The need for alternative treatments for pests mitigation systems is greater than ever. Global trade pressures and the possible loss of methyl bromide make it imperative that all practical treatment options be explored. Since 1989 irradiation treatment concepts have matured significantly. Technological advances, greater experience, and an increasingly larger body of research indicate that irradiation has important potential as a treatment for quarantine pest problems. It is in this light that PPQ is expanding its regulatory framework, is addressing irradiation treatment options, and is developing comprehensive policy statements intended to facilitate the development and formalization of new treatments for phyto sanitary applications. (Author)

The application of irradiation to phyto sanitary problems

International Nuclear Information System (INIS)

Ross, R.T.

1997-01-01

The first formally adopted regulatory policy for irradiation as a phyto sanitary treatment in the United States was issued in 1989 and was based on Title 7 of the Code of Federal Regulations. These regulations authorized irradiation as a quarantine treatment for papayas intended for movement from the State of Hawaii to the continental United States (U.S.), Guam, Puerto Rico, and the U.S. Virgin Islands. This authorization was specific for commodity, place of origin, and program, but was designed for a complex of three fruit flies rather than a single pest. Routine commercial shipments were never realized under this regulation due to the lack of a treatment facility in Hawaii. However, the authorization has proven useful from the standpoint of beginning to establish policies for irradiation as a phyto sanitary treatment in the United States. The United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ) remains dedicated to using the most up-to-date, appropriate and least intrusive technology to provide quarantine security. The need for alternative treatments for pests mitigation systems is greater than ever. Global trade pressures and the possible loss of methyl bromide make it imperative that all practical treatment options be explored. Since 1989 irradiation treatment concepts have matured significantly. Technological advances, greater experience, and an increasingly larger body of research indicate that irradiation has important potential as a treatment for quarantine pest problems. It is in this light that PPQ is expanding its regulatory framework, is addressing irradiation treatment options, and is developing comprehensive policy statements intended to facilitate the development and formalization of new treatments for phyto sanitary applications. (Author)

The application of irradiation to phyto sanitary problems

Energy Technology Data Exchange (ETDEWEB)

Ross, R.T. [USDA/APHIS/PPQ. Department of Agriculture, Room 1630 Soagribg, 1400 Independence Ave. Sw. Mail Code Stop 3438, 20250 Washington D.C. (United States)

1997-12-31

The first formally adopted regulatory policy for irradiation as a phyto sanitary treatment in the United States was issued in 1989 and was based on Title 7 of the Code of Federal Regulations. These regulations authorized irradiation as a quarantine treatment for papayas intended for movement from the State of Hawaii to the continental United States (U.S.), Guam, Puerto Rico, and the U.S. Virgin Islands. This authorization was specific for commodity, place of origin, and program, but was designed for a complex of three fruit flies rather than a single pest. Routine commercial shipments were never realized under this regulation due to the lack of a treatment facility in Hawaii. However, the authorization has proven useful from the standpoint of beginning to establish policies for irradiation as a phyto sanitary treatment in the United States. The United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ) remains dedicated to using the most up-to-date, appropriate and least intrusive technology to provide quarantine security. The need for alternative treatments for pests mitigation systems is greater than ever. Global trade pressures and the possible loss of methyl bromide make it imperative that all practical treatment options be explored. Since 1989 irradiation treatment concepts have matured significantly. Technological advances, greater experience, and an increasingly larger body of research indicate that irradiation has important potential as a treatment for quarantine pest problems. It is in this light that PPQ is expanding its regulatory framework, is addressing irradiation treatment options, and is developing comprehensive policy statements intended to facilitate the development and formalization of new treatments for phyto sanitary applications. (Author)

Effect of linear alkylbenzene mixtures and sanitary sewage in biochemical and molecular responses in pacific oyster Crassostrea gigas.

Science.gov (United States)

Flores-Nunes, Fabrício; Mattos, Jacó J; Zacchi, Flávia L; Serrano, Miguel A S; Piazza, Clei E; Sasaki, Silvio T; Taniguchi, Satie; Bicego, Márcia C; Melo, Cláudio M R; Bainy, Afonso C D

2015-11-01

Urban effluents are rich in nutrients, organic matter, pharmaceuticals and personal care products (PPCPs), pesticides, hydrocarbons, surfactants, and others. Previous studies have shown that oysters Crassostrea gigas accumulate significant levels of linear alkylbenzenes (LABs) in sanitary sewage contaminated sites, but there is little information about its toxicological effects in marine bivalves. The aim of this study was to analyze the transcription of genes in two tissues of C. gigas exposed for 12, 24, and 36 h to LABs or sanitary sewage. Likewise, the activity of antioxidant and biotransformation enzymes was measured in oysters exposed for 36 h in all groups. Oysters exposed to LABs and oysters exposed to sanitary sewage showed different patterns of transcriptional responses. LAB-exposed oysters showed lower level of biological responses than the oysters exposed to sanitary sewage. Despite the ability of the oyster C. gigas to accumulate LABs (28-fold), the data indicate that these contaminants are not the cause for the transcriptional responses observed in oysters exposed to sanitary sewage. Possibly, the biological changes observed in the sanitary sewage-exposed oysters are associated with the presence of other contaminants, which might have caused synergistic, additive, or antagonistic effects. The results show that FABP-like and GST-ω-like messenger RNAs (mRNAs) have a rapid response in tissues of oyster C. gigas exposed to sanitary sewage, suggesting a possible protective response and a role in maintaining homeostasis of these organisms.

In vitro hatching of Trichuris suis eggs

DEFF Research Database (Denmark)

Vejzagic, Nermina; Thamsborg, Stig Milan; Kringel, Helene

2015-01-01

Eggs of the pig whipworm, Trichuris suis ova (TSO), are currently tested in human clinical trials for their potential immunomodulatory capacity. The biological potency of TSO (egg viability and infectivity) is traditionally assessed in Göttingen minipigs as the establishment of intestinal larvae...... after inoculation with a known number of eggs. To minimize testing in animal models, development of an in vitro egg hatching assay is proposed as a reliable, cost-effective, and a faster alternative to test the egg viability. The present study aimed to investigate the influence of different chemical......, physical, and biological factors on egg hatching. Thus, in a series of experiments and in different combinations, the eggs were stimulated with glass beads, artificial gastric juice, bile salt and trypsin solution, fermentation gut medium, or stimulated with mucosal scrapings from the ileum and the large...

DIPLOMASI TAKHTA SUCI SEBAGAI SUBJEK HUKUM INTERNASIONAL SUI GENERIS

Directory of Open Access Journals (Sweden)

Agustinus Supriyanto

2015-02-01

Full Text Available The Holy See is different from the Vatican. The Vatican is a state, whereas the Holy See is the central governing institution of the Catholic Church. The position of the Holy See is in the Vatican State. The international status of the Holy See is very unique. It is a sui generis subject of international law. Although it is not a state, it enjoys international recognition. Its diplomacy consists of intern and extern aspect. The intern aspect of its diplomacy is that of church interest. The extern aspect is that of diplomacy itself. It is neutral in internationally political interests. It focuses on humanitarian and moral diplomacy.

Cell-mediated immune responses differentiate infections with Brucella suis from Yersinia enterocolitica serotype O : 9 in pigs

DEFF Research Database (Denmark)

Riber, Ulla; Jungersen, Gregers

2007-01-01

Due to almost identical lipopolysaccharide (LPS) O-antigens, infections with Yersinia enterocolitica serotype 0:9 (YeO:9) cause false positive serological reactions (FPSR) in tests for Brucella and thus cause problems in National Brucella surveillance programs. As LPS are strong inducers...... of antibody responses it was hypothesized that cell-mediated immune responses to non-LPS antigens of the two bacteria can be used to separate immune responses to these two biologically very different infections. Following subclinical experimental infections with Brucella suis biovar 2, high interferon......-gamma (IFN-gamma) assay responses with a commercial Brucella melitensis antigen preparation (Brucellergene OCB) preceded the development of antibodies. High IFN-gamma responses in the seven B. suis inoculated pigs with serological evidence of infection were consistent throughout a 20-week postinoculation...

Genetic analysis of Trichuris suis and Trichuris trichiura recovered from humans and pigs in a sympatric setting in Uganda

DEFF Research Database (Denmark)

Nissen, Sofie; Al-Jubury, Azmi; Hansen, Tina Vicky Alstrup

2012-01-01

The whipworms Trichuris trichiura and Trichuris suis in humans and pigs, respectively, are believed to be two different species yet closely related. Morphologically, adult worms, eggs and larvae of the two species are indistinguishable. The aim of this study was to examine the genetic variation....... suis-type) and three cases of ‘heterozygote’ worms in humans were identified. However, the analysis showed that sympatric Trichuris primarily assorted with host origin. Sequence analysis of a part of the genetically conserved ¿-tubulin gene confirmed two separate populations/species but also showed...... that the ‘heterozygote’ worms had a T. suis-like ¿-tubulin gene. A PCR-RFLP on the ITS-2 region was developed, that could distinguish between worms of the pig, human and ‘heterozygote’ type. The data suggest that Trichuris in pigs and humans belong to two different populations (i.e. are two different species). However...

Sanitary services in the perm region during the Great Patriotic war

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V.A. Horoshavin

2015-06-01

Full Text Available The article is devoted to the history of the formation of the Medical Service of the (Molotov region during the war. We describe the problems encountered sanitary doctors in the war years, the direction of the main measures to address these problems. It is shown that the organizers of the health service – A.V. Kostina, V.A. Ryazanov, their colleagues have made every effort to prevent epidemics and save health of both local and evacuees. It is showed the role of volunteers, doctors of medical units at the plants, the Department of Health Medical Institute to improve the sanitary conditions in the region.

Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe

Czech Academy of Sciences Publication Activity Database

Němejc, K.; Sak, Bohumil; Květoňová, Dana; Hanzal, V.; Janiszewski, P.; Forejtek, P.; Rajský, D.; Ravaszová, P.; McEvoy, J.; Kváč, Martin

2013-01-01

Roč. 197, 3-4 (2013), s. 504-508 ISSN 0304-4017 Grant - others:Jihočeská univerzita(CZ) 022/2010/Z; Jihočeská univerzita(CZ) 11/2013/Z Institutional support: RVO:60077344 Keywords : Central Europe * Cryptosporidium scrofarum * Cryptosporidium suis * Eurasian wild boar * PCR * SSU Subject RIV: EE - Microbiology, Virology Impact factor: 2.545, year: 2013

Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014.

Science.gov (United States)

Hernandez-Garcia, Juan; Wang, Jinhong; Restif, Olivier; Holmes, Mark A; Mather, Alison E; Weinert, Lucy A; Wileman, Thomas M; Thomson, Jill R; Langford, Paul R; Wren, Brendan W; Rycroft, Andrew; Maskell, Duncan J; Tucker, Alexander W

2017-08-01

Antimicrobial resistance in Streptococcus suis, a global zoonotic pathogen of pigs, has been mostly studied only in diseased animals using surveys that have not evaluated changes over time. We compared patterns of resistance between S. suis isolates from clinical cases of disease (CC) and non-clinical case (NCC) pigs in England, collected over two discrete periods, 2009-2011 and 2013-2014. Minimum inhibitory concentrations (MIC) of 17 antimicrobials (nine classes) were determined on 405 S. suis isolates categorised by sampling period and disease association to assess changes in resistance over time and association with disease. First, isolates were characterized as resistant or susceptible using published clinical breakpoints. Second, epidemiological cut-offs (ECOFF) were derived from MIC values, and isolates classified as wild type (WT) below the ECOFF and non-wild type (NWT) above the ECOFF. Finally, isolate subsets were analysed for shifts in MIC distribution. NCC isolates were more resistant than CC isolates to cephalosporins, penams, pleuromutilins, potentiated sulphonamides and tetracyclines in both study periods. Resistance levels among CC isolates increased in 2013-2014 relative to 2009-2011 for antimicrobials including aminoglycosides, cephalosporins, fluoroquinolones, pleuromutilins, potentiated sulphonamides and tetracyclines. The prevalence of isolates categorised as NWT for five or more classes of antimicrobials was greater among NCC than CC isolates for both time periods, and increased with time. This study used standardised methods to identify significant shifts in antimicrobial resistance phenotypes of S. suis isolated from pigs in England, not only over time but also between isolates from known clinical cases or disease-free pigs. Copyright © 2017. Published by Elsevier B.V.

A Hashtag Worth a Thousand Words: Discursive Strategies Around #JeNeSuisPasCharlie After the 2015 Charlie Hebdo Shooting

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Fabio Giglietto

2017-01-01

Full Text Available Following a shooting attack by two self-proclaimed Islamist gunmen at the offices of French satirical weekly Charlie Hebdo on 7 January 2015, there emerged the hashtag #JeSuisCharlie on Twitter as an expression of solidarity and support for the magazine’s right to free speech. Almost simultaneously, however, there was also #JeNeSuisPasCharlie explicitly countering the former, affirmative hashtag. Based on a multimethod analysis of 74,047 tweets containing #JeNeSuisPasCharlie posted between 7 and 11 January, this article reveals that users of the hashtag under study employed various discursive strategies and tactics to challenge the mainstream framing of the shooting as the universal value of freedom of expression being threatened by religious extremism, while protecting themselves from the risk of being viewed as disrespecting victims or endorsing the violence committed. The significance of this study is twofold. First, it extends the literature on strategic speech acts by examining how such acts take place in a social media context. Second, it highlights the need for a multidimensional and reflective methodology when dealing with data mined from social media.

Long-term outcomes of patients with Streptococcus suis infection in Viet Nam: A case-control study.

Science.gov (United States)

Huong, Vu T L; Long, Hoang B; Kinh, Nguyen V; Ngan, Ta T D; Dung, Vu T V; Nadjm, Behzad; van Doorn, H Rogier; Hoa, Ngo T; Horby, Peter; Wertheim, Heiman F L

2018-02-01

Streptococcus suis is a zoonotic cause of severe meningitis and sepsis in humans. We aimed to assess the long-term outcomes in patients who survived S. suis infection, in particular the progress and impact of vestibulocochlear sequelae. This case-control study evaluated outcomes of S. suis infection at discharge and 3 and 9 months post-discharge for 47 prospectively enrolled cases and at 11-34 months for 31 retrospectively enrolled cases. Outcomes in patients were compared to 270 controls matched for age, sex and residency. The prevalence ratio (PR) of moderate-to-complete hearing loss was 5.0(95%CI 3.6-7.1) in cases at discharge, 3.7(2.5-5.4) at 3 months, 3.2(2.2-4.7) at 9 months, and 3.1(2.1-4.4) in retrospective cases compared to controls. Hearing improvement occurred mostly within the first 3 months with a change in hearing level of 11.1%(95%CI 7.0-15.1%) compared to discharge. The PR of vestibular dysfunction was 2.4(95%CI 1.7-3.3) at discharge, 2.2(1.4-3.1) at 3 months, 1.8(1.1-2.5) at 9 months, and 1.8(1.1-2.6) for retrospective cases compared to controls. Cases also indicated more problems with mobility, self-care and usual activities. Both hearing and vestibular impairment were common and persist in cases. Appropriate patient management strategies are needed to reduce the incidence and impact of these sequelae. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

The Outcome of Repeated Mid Urethral Sling in SUI Treatment after Vaginal Excisions of Primary Failed Sling: Preliminary Study

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Jacek Kociszewski

2016-01-01

Full Text Available Mid urethral sling is the standard in SUI treatment. Nevertheless, the risk of reoperation reaches 9%. There is no consensus as to the best treatment option for complications. A question is raised: what is the optimal way to achieve the best result in patients after primary failure? The aim of the study was to evaluate the outcomes of repeat MUS surgery in patients after excision of the sling with recurrent SUI. We compared its effectiveness with uncomplicated cases treated with TVT. 27 patients who underwent the repeated MUS and 50 consecutive patients after primary TVT were enrolled in the study. After 6 months, we have found that 24 (88.46% patients from repeat sling group and 48 (96% patients after primary sling were dry (1-hour pad test, 2 g or less. The difference between groups was not significant. We showed statistically significant improvement of quality of life in both groups. In conclusion, we showed that repeated sling after MUS excision is almost as effective as primary MUS. We postulate that sling excision and repeated MUS may be the best option for persistent SUI and/or complications after MUS procedures. Further multicenter observations are ongoing as to provide results on bigger group of cases.

Conceptual design report, 200 Area sanitary sewer system: Project 96L-EWL-116

International Nuclear Information System (INIS)

Pursley, D.L.

1994-01-01

Project L-116 will install an integrated sanitary sewer system in the 200 Area. This new system will connect existing sewer systems for facilities that have a foreseeable future, provide capacity and routing for future facilities, and install new septic sewer systems for existing facilities that cannot be feasibly connected to the new sewer system and have a mission that will extend beyond the year 2000. Project L-116 will construct a sanitary sewer collection, treatment, and disposal system for facilities in the 200-East and -West Areas and adjacent areas located on the 200 Area plateau. The existing septic systems will be abandoned or decommissioned in accordance with applicable Washington State and local codes and regulations. The conceptual design for the sanitary sewer system is designed around population forecasts of 5,000 people for 200-West Area and 9,000 people for 200-East Area. The definitive design will be based on the latest forecast populations at the time definitive design is initiated

Solidarity: an innovative perspective in the management and organization of Sanitary Surveillance actions.

Science.gov (United States)

Leal, Cristian Oliveira Benevides Sanches; Teixeira, Carmen Fontes de Souza

2017-10-01

This is a theoretical essay about the development of the concept of solidarity, a word used in the regulatory framework and in political proposals to reorient the Brazilian Unified Health System (SUS). The methodology consisted of mapping authors addressing human action aspects related to this theme from Durkheim's tradition, linking them to his followers, like Marcel Mauss and authors from the "anti-utilitarianism" movement in social sciences. Solidarity is one way to express a "gift" and appears as a multidimensional action, where duty and freedom, instrumental interest and disinterest interpose and interlace. The planning and execution of sanitary surveillance (VISA) actions requires comprehension of organizational forms and solidary relationship management among agents involved in health risk control, transcending the strongly normative aspect of the prevailing supervision actions. The development of associative actions involving sanitary surveillance professionals, economic agents and consumers, aiming to share the responsibilities in the health risk control of products, services and environments subjected to Sanitary Surveillance action is suggested.

Immunogenicity and efficacy of a rough Brucella suis vaccine delivered orally or parenterally to feral swine

Science.gov (United States)

Brucella suis strain 353-1 is a stable vaccine strain that is clinically safe, does not cause positive serologic responses on conventional brucellosis surveillance tests, and induces humoral and cellular immunity in swine after vaccination. In this study, we evaluated tissue clearance and immunologi...

[Power and health in South America: international sanitary conferences, 1870-1889].

Science.gov (United States)

Chaves, Cleide de Lima

2013-06-01

This article analyzes the international sanitary conferences that were held in South America in 1873 and 1887, involving the Brazilian Empire and the Republics of Argentina and Uruguay, as an integral part of a series of similar events that took place in Europe and North America starting in the second half of the nineteenth century. The interests of the countries involved, namely trade relations and immigration from Europe - both directly affected by the epidemics - are discussed, and the repercussions of these sanitary agreements on the other countries in the Americas are indicated. The American health conventions in the late nineteenth century represented the first initiatives in the Americas to solve international public health problems.

FROM IPAB TO ASP: THE EVOLUTION OF DECISIONAL PROCESSES

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Dario Recubini

2014-12-01

Full Text Available This study aims to analyze the evolution of decision-making processes through the transformation of The Public Institution of Assistance and Charity (IPAB, in Public Corporations aimed to the Person (ASP. Public corporations of services oriented to the Person (ASP are, in facts, an innovation. In the Public sector, ASPs are of big relevance, providing health care services to the society and taking a new and innovative governance model. This model wants to increase he awareness of ASP’s role in the public sector and generate awareness of the social effects, coupled with cost effectiveness and competitiveness. The public-law Corporations, which have got legal subject, administrative, property, accounting and financial autonomy, perform their activity with efficiency, efficacy and cost effectiveness, keeping the right balance between costs and profits. They are also able to save, anyway, not less than a third of incomings to realize various activities. These kind of companies are active at council and provincial level. This process is managed by the Region which endorses its Statutes, promotes the establishment of tools for the valuation of property assets and regulates the information and accounting system.   DALLE IPAB ALLE ASP: L’EVOLUZIONE DEI PROCESSI DECISIONALI Lo studio intende analizzare l’evoluzione dei processi decisionali nella trasformazione delle Istituzioni Pubbliche di Assistenza e Beneficienza (IPAB in Aziende Servizi alla Persona (ASP. Le Aziende pubbliche di servizi alla persona (ASP rappresentano, infatti,  un soggetto innovativo di grande rilevanza nell'ambito dei soggetti pubblici produttori ed erogatori di servizi socio-sanitari assumendo un nuovo modello di governance innovativo, diretto ad aumentare la consapevolezza del ruolo svolto e degli effetti prodotti in termini sociali, economici e competitivi. Aziende di diritto pubblico, dotate di personalità giuridica, di autonomia statutaria, gestionale, patrimoniale

Epidemiological investigation of the first human brucellosis case in Spain due to Brucella suis biovar 1 strain 1330.

Science.gov (United States)

Compés Dea, Cecilia; Guimbao Bescós, Joaquín; Alonso Pérez de Ágreda, Juan Pablo; Muñoz Álvaro, Pilar María; Blasco Martínez, José María; Villuendas Usón, María Cruz

2017-03-01

No cases of human brucellosis caused by Brucella suis has been reported in Spain. This study involved interviews with the case and his co-workers, inspection of their workplace, checking infection control measures, and typing the Brucella strain isolated in the blood culture. Brucella suis biovar 1 strain 1330 was isolated from a patient who worked in a waste treatment plant. Food borne transmission, contact with animals, and risk jobs were ruled out. An accidental inoculation with a contaminated needle from a research laboratory waste container was identified as the most probable mode of transmission. There should be controls to ensure that waste containers are sealed. Copyright © 2016 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

Effects of supplement with sanitary landfill leachate in gas exchange of sunflower (Helianthus annuus L.) seedlings under drought stress.

Science.gov (United States)

Nunes Junior, Francisco H; Freitas, Valdineia S; Mesquita, Rosilene O; Braga, Brennda B; Barbosa, Rifandreo M; Martins, Kaio; Gondim, Franklin A

2017-10-01

Sanitary landfill leachate is one of the major problems arising from disposal of urban waste. Sanitary landfill leachate may, however, have use in agriculture. This study, therefore, aimed to analyze initial plant growth and gas exchange in sunflower seedlings supplemented with sanitary landfill leachate and subjected to drought stress through variables of root fresh mass (RFM), shoot fresh mass (SFM), total fresh mass (TFM), relative chlorophyll content (CL), stomatal conductance (g s ), transpiration rate (E), net photosynthetic rate (A), ratio of internal to external CO 2 concentration (Ci/Ca),water use efficiency (EUA), instantaneous carboxylation efficiency (A/Ci), and electron transport rate (ETR). The experimental design was a completely randomized 2 (irrigated and non-irrigated) × 4 (sand, sand + 100 kg N ha -1 organic fertilizer, sand + 100 kg N ha -1 sanitary landfill leachate, and sand + 150 kg N ha -1 sanitary landfill leachate) factorial with five replicates. Under drought stress conditions, leachate treatment supplemented with 100 kg N ha -1 exhibited higher plant fresh weights than those of the treatment containing 150 kg N ha -1 . Increases in fresh mass in plant treatments supplemented with 100 and 150 kg N ha -1 sanitary landfill leachate were related to higher photosynthetic rates.

MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

Science.gov (United States)

Tan, Mei-Fang; Gao, Ting; Liu, Wan-Quan; Zhang, Chun-Yan; Yang, Xi; Zhu, Jia-Wen; Teng, Mu-Ye; Li, Lu; Zhou, Rui

2015-01-01

Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

40 CFR 141.723 - Requirements to respond to significant deficiencies identified in sanitary surveys performed by EPA.

Science.gov (United States)

2010-07-01

... deficiencies identified in sanitary surveys performed by EPA. 141.723 Section 141.723 Protection of Environment... performed by EPA, systems must respond in writing to significant deficiencies identified in sanitary survey... will address significant deficiencies noted in the survey. (d) Systems must correct significant...

Considering the sanitary aspects in regional plans for air quality. Situation of sanitary impacts of urban air pollution studies; Prise en compte des aspects sanitaires dans les Plans regionaux pour la qualite de l'air. Bilan des etudes d'impact sanitaires de la pollution atmospherique urbaine realisees

Energy Technology Data Exchange (ETDEWEB)

NONE

2003-12-15

The law on air and the rational use of energy of the 30. september 1996 forecasts the setting up of regional planning for the air quality that have to rely on the support of an evaluation of sanitary effects of air pollution. To help the local sanitary authorities in this mission, the National Institute of Sanitary Surveillance and the C.I.R.E. have realised a methodological guide on evaluation of sanitary impact of urban air pollution in different contexts. (N.C.)

Serotype- and virulence-associated gene profile of Streptococcus suis isolates from pig carcasses in Chiang Mai Province, Northern Thailand.

Science.gov (United States)

Wongsawan, Kanruethai; Gottschalk, Marcelo; Tharavichitkul, Prasit

2015-02-01

In this present study, the serotype of 40 Streptococcus suis isolates from submaxillary glands of pig carcasses sold in wet markets in Chiang Mai Province, northern Thailand, was investigated. Eleven serotypes, including types 2, 3, 4, 5, 7, 8, 9, 17, 21, 22 and 31, were found in the isolates by a Multiplex PCR combined with serum agglutination. Of the eleven serotypes present, type 3 was the most prevalent, while types 2, 4, 5 and 21 were of primary interest due to their human isolate serotype. The mrp+/epf - /sly - genotype was found to be the most prevalent genotype. This study indicates the importance of effective control of human S. suis infection due to raw pork or pig carcass handling in northern Thailand.

Methodological issues of optimization the sanitary-educational assistance for children with asthma during the health care reform of Ukraine

Directory of Open Access Journals (Sweden)

S. N. Nedelskaya

2013-08-01

Full Text Available Entry. Asthma remains the medical, social, economic issue of the day of modern society and industry of health protection. Research works on questions asthma are sanctified to mainly the improvement of diagnostics and treatment of asthma and a sanitary-educational help is underestimated. In the modern terms of structural alteration of network of establishments of health care in Ukraine a sanitary-educational help must be an effective complex in strategy of therapy of patients with asthma. Research aim. To ground importance, modern forms and methods of sanitary -educational help in providing of control above asthma on the stage of structural reorganization of establishments of health care in Ukraine. Materials and research methods. Scientifically-methodical literature was analyzed on general questions of organization and realization of sanitary-educational work in asthma. Long-term of own experience of realization of this work is generalized in the conditions of stationary treatment of patients with asthma. Research results. Sanitary-educational work in a form of education in the program "Asthma school". General practitioner, as a key figure of establishments of primary medical and sanitary help, must own the psychological methods including medical-psychology programs of before- and after graduation medical education. Conclusions. In providing of effective control above bronchial asthma in children a sanitary-educational help must be logical continuation and addition of curative help. Application of the educational programs and volume of work of doctors at their implementation must be legislatively lighted up in "Protocols of diagnostics and treatment of bronchial asthma for children".

Diagnosis of porcine enzootic pneumonia by post mortem sanitary inspection: comparison with other diagnostic methods

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Kênia de Fátima Carrijo

2014-06-01

Full Text Available ABSTRACT. Carrijo K.F., Nascimento E.R., Pereira V.L.A., Morés N., Klein, C.S., Domingues L.M. & Tortelly R. [Diagnosis of porcine enzootic pneumonia by post mortem sanitary inspection: comparison with other diagnostic methods.] Diagnóstico da pneumonia enzoótica suína pela inspeção sanitária post mortem: comparação com outros métodos de diagnóstico. Revista Brasileira de Veterinária Brasileira 36(2:188-194, 2014. Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Pará, 1720, Bloco 2T, Jardim Umuarama, Uberlândia, MG 38400-902, Brasil. E-mail: keniacarrijo@ famev.ufu.br To compare the concordance of the diagnosis of porcine enzootic pneumonia (PEP by post-mortem Sanitary Inspection with other methods (histophatology and immunohistochemistry - IHC, were used lung tissue samples from 100 pigs slaughtered under sanitary inspection, and 50 of these had macroscopic lesions suggestive of PEP and 50 had no such lesions. These were fixed in 10% buffered formalin and processed by routine procedures for paraffin embedding and IHC technique for Mycoplasma hyopneumoniae using a monoespecific polyclonal antibody. The study demonstrating that there is concordance between the diagnosis of Sanitary Inspection with histophatology, between the diagnosis of Sanitary Inspection with IHC and histophatology with IHC. It can be conclude that when the lung has gross lesions of PEP, the probability the result is positive to M. hyopneumoniae by IHC and the presence of microscopic lesions increases. Thus, the microscopic diagnosis for PEP is feasible because it is associated to the other, so that the diagnosis given by the officials of Sanitary Inspection in slaughterhouses is not wrong; the macroscopic diagnosis is therefore a valid method for the diagnosis of PEP, it being understood this is not to say that the detection of M. hyopneumoniae.

[Evaluation of the hygienic-sanitary conditions of kitchens in public and philanthropic daycare centers].

Science.gov (United States)

Oliveira, Mariana de Novaes; Brasil, Anne Lise Dias; Taddei, José Augusto de Aguiar Carrazedo

2008-01-01

This paper aims to evaluate the hygienic-sanitary conditions of kitchens in philanthropic and public daycare centers in the city of São Paulo using a tool of easy application. Information on the hygienic-sanitary conditions was gathered observing the operational conditions of five public and philanthropic daycare centers in the city of São Paulo. A score was developed for classifying the risks of food contamination. The operational conditions in the kitchens of the studied philanthropic and public daycare centers in the city of São Paulo can result in contamination of the prepared food. Among the most important risk factors for food contamination is the behavior of the workers who handle the food. Training and continuous supervision of the involved personnel are the best and easiest alternatives for assuring the appropriate hygienic-sanitary conditions and quality of the food offered to the children in these daycare centers.

Effect of Early-Life Treatment of Piglets with Long-Acting Ceftiofur on Colonization of Streptococcus suis Serotype 7 and Elicitation of Specific Humoral Immunity in a Farm Dealing with Streptococcal Diseases

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Christine Unterweger

2018-03-01

Full Text Available In newborn piglets treatment with long-acting ceftiofur is a common approach to reduce losses due to streptococcal diseases on farms, even if problems start after weaning. The purpose of this study was to examine the influence of a single early-life treatment on Streptococcus (S. suis colonization, transmission, immunoreaction, and drug resistance over an observation period of 14 weeks. In a farm with a history of streptococcal disease and isolation of a S. suis cps 7 mrp+, arcA+ isolate from diseased piglets, half of each litter was treated with a long-acting ceftiofur on day 1. S. suis-isolates were profiled and serum samples were tested for opsonizing antibodies. Treated and untreated pigs did not differ according to average daily weight gains, S. suis-isolation rates and level of opsonizing antibodies. Although the invasive cps 7 strain was not detected in a single piglet over 14 weeks, all animals developed bactericidal activity. No resistance to ceftiofur, but resistance to tetracyclins (100%, and trimethoprim/sulfamethoxazole (53% was shown. Our results indicate that early treatment with ceftiofur does not prevent colonization and transmission of S. suis or the induction of bactericidal humoral immunity in nursery and fattening pigs. The necessity of continuous usage should be reconsidered.

Virulence Studies of Different Sequence Types and Geographical Origins of Streptococcus suis Serotype 2 in a Mouse Model of Infection

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Jean-Philippe Auger

2016-07-01

Full Text Available Multilocus sequence typing previously identified three predominant sequence types (STs of Streptococcus suis serotype 2: ST1 strains predominate in Eurasia while North American (NA strains are generally ST25 and ST28. However, ST25/ST28 and ST1 strains have also been isolated in Asia and NA, respectively. Using a well-standardized mouse model of infection, the virulence of strains belonging to different STs and different geographical origins was evaluated. Results demonstrated that although a certain tendency may be observed, S. suis serotype 2 virulence is difficult to predict based on ST and geographical origin alone; strains belonging to the same ST presented important differences of virulence and did not always correlate with origin. The only exception appears to be NA ST28 strains, which were generally less virulent in both systemic and central nervous system (CNS infection models. Persistent and high levels of bacteremia accompanied by elevated CNS inflammation are required to cause meningitis. Although widely used, in vitro tests such as phagocytosis and killing assays require further standardization in order to be used as predictive tests for evaluating virulence of strains. The use of strains other than archetypal strains has increased our knowledge and understanding of the S. suis serotype 2 population dynamics.

The Sanitary Conditions of Food Service Establishments and Food ...

African Journals Online (AJOL)

Sixty six percent of the establishments had flush toilets whereas 5.9% of the establishment had no toilet. Only 149 (33.6%) of the establishments had a proper solid waste collection receptacle and there was statistically significant association between the sanitary conditions and license status of the establishments (p=0.01).

NORTH PORTAL-SANITARY SEWER CALCULATION-SHOP BUILDING No.5006

International Nuclear Information System (INIS)

S. Blackstone

1996-01-01

The purpose of this design calculation is to determine the demand on the waste system and to size the sanitary sewer line serving the Shop Building No.5006 in accordance with the Uniform Plumbing Code (Section 4.4.1) and US Department of Energy Order 6430.1A-1540 (Section 4.4.2)

Nutrition of pigs kept under low and high sanitary conditions

NARCIS (Netherlands)

Meer, van der Yvonne

2017-01-01

It is economically and environmentally important to match the nutrient supply to the nutrient requirements in pig production. Until now, the effects of different sanitary conditions on energy and nutrient requirements are not implemented in recommendations for nutrient composition of pig diets.

49 CFR Appendix C to Part 228 - Guidelines for Clean, Safe, and Sanitary Railroad Provided Camp Cars

Science.gov (United States)

2010-10-01

... steady sound level which in 8 hours would contain the same acoustic energy as the time-varying sound... changed regularly. 7. Lavatories. (a) Lavatories should be made available to all rail employees housed in... sanitary condition. (e) Sanitary storage. No food or beverages should be stored in toilet rooms or in an...

Long-term clinical outcomes with the retropubic tension-free vaginal tape (TVT) procedure compared to Burch colposuspension for correcting stress urinary incontinence (SUI).

Science.gov (United States)

Holdø, Bjørn; Verelst, Margareta; Svenningsen, Rune; Milsom, Ian; Skjeldestad, Finn Egil

2017-11-01

The retropubic tension-free vaginal tape (TVT) procedure replaced Burch colposuspension as the primary surgical method for stress urinary incontinence (SUI) and mixed urinary incontinence (MUI) in women in our department in 1998. In this study we compared the short-term and long-term clinical outcomes of these surgical procedures. Using a case series design, we compared the last 5 years of the Burch procedure (n = 127, 1994-1999) with the first 5 years of the retropubic TVT procedure (n = 180, 1998-2002). Information from the medical records was transferred to a case report form comprising data on perioperative and long-term complications as well as recurrence of UI, defined as bothersome UI or UI in need of repeat surgery. Other endpoints were rates of perioperative and late complications and the rates of prolapse surgery after primary surgery. The data were analyzed with the chi-squared and t tests and survival analysis using SPSS. The cumulative recurrence rate of SUI in women with preoperative SUI was significantly higher after the Burch procedure, but no difference was observed in women with MUI. There were no significant differences in rates of perioperative and late complications. At 12 years there was a significant increase in rates of repeat surgery for incontinence and prolapse in women after the Burch procedure. The long-term efficacy of TVT surgery was superior to that of Burch colposuspension in women with SUI. In addition, the rate of late prolapse surgery was significantly higher after the Burch procedure.

Immuogenicity and safety of a natural rough mutant of Brucella suis as a vaccine for swine

Science.gov (United States)

The objective of the current study was to evaluate the safety, immunogenicity and clearance of the natural rough mutant of Brucella suis strain 353-1 (353-1) as a vaccine in domestic swine. In three studies encompassing 155 animals, pigs were inoculated with 353-1 by conjunctival (5 x 10**7 CFU), p...

Relativistic electron influence on sanitary-model microorganisms and antibiotics in model samples

International Nuclear Information System (INIS)

Antipov, V.S.; Berezhna, I.V.; Kovpik, O.F.; Babych, E.M.; Voliansky, Yu.L.; Sklar, N.I.

2004-01-01

A series of the investigations of the electron beam influence on sanitary-model test cultures and antibiotics in model solutions has been carried out. For each of the test objects, the authors have found the boundary doses of the absorbed radiation. The higher doses cause the sharp increase in the bactericidal influence, which becomes complete. The sanitary-bactericidal indices of the water samples remain sable during 6 days. The samples of antibiotics in various concentrations (from 100 UA) have been irradiated. It is proved that the substratum processing by the beam (in the regimes 30 kGy) causes diminution and complete neutralization of the antibacterial activity in all probes of the samples

Conducting Sanitary Surveys of Water Supply Systems. Student Workbook.

Science.gov (United States)

1976

This workbook is utilized in connection with a 40-hour course on sanitary surveys of water supply systems for biologists, chemists, and engineers with experience as a water supply evaluator. Practical training is provided in each of the 21 self-contained modules. Each module outlines the purpose, objectives and content for that section. The course…

Assessing the zoonotic potential of Ascaris suum and Trichuris suis

DEFF Research Database (Denmark)

Nejsum, Peter; Betson, M.; Bendall, R. P.

2012-01-01

The two geohelminths, Ascaris lumbricoides and Trichuris trichiura, infect more than a billion people worldwide but are only reported sporadically in the developed part of the world. In contrast, the closely related species A. suum and T. suis in pigs have a truly global distribution, with infect...... and pig host, with special focus on recent evidence concerning the zoonotic potential of these parasites, and identify some open questions for future research....

[Bladder injury during sling operation in the treatment of SUI--review of literature and case report].

Science.gov (United States)

Gałczyński, Krzysztof; Futyma, Konrad; Bar, Krzysztof; Rechberger, Tomasz

2012-10-01

Sling operations have been performed for over 15 years. In recent years these operations have become the gold standard in the treatment of stress urinary incontinence (SUI) due to their efficacy safety and low invasiveness. Approximately 4% of women will undergo a surgery for SUI in the course of their life. As with any surgical intervention, there may be some technical problems, as well as intra- and postoperative complications, the most common of which is bladder injury Other complications encountered during mid-urethral slings procedures include bleeding (retropubic or vaginal hematomas), urethral perforation, urinary tract infections, postoperative vaginal or urethral erosions, bowel perforation, chronic pelvic pain, wound infection, nerve injury transient and persistent voiding dysfunction such as de novo urgency incomplete bladder emptying or urinary retention. Below we present a case of a patient with diagnosed vesicovaginal fistula after sling operation (TVT-tension-free vaginal tape). Upon admission the patient reported dysuria, persistent urinary leakage and abnormal, abundant vaginal discharge. Case report and review of literature concerning surgical treatment of stress urinary incontinence and its complications. Analysis of medical documentation of the patient treated at the Second Department of Gynecology Medical University of Lublin. Review of abstracts or papers in the Medline database related to surgical treatment of urinary incontinence and its complications. Bladder perforation is one of the most common complications of the retropubic approach for MUS placement. The presence of mesh within the bladder may arise from direct bladder perforation or from subsequent erosion of the sling. Such lesions do not cause any serious health consequences for patients on condition they are detected intraoperatively and appropriately repaired, but when unrecognized, they results in the development of considerable symptoms and negatively influence the quality of

Une étude de cas. Saint Sébastien dans “Je suis sang” de Jan Fabre

Directory of Open Access Journals (Sweden)

Lydie Toran

2017-06-01

Full Text Available Jan Fabre’s writing frequently displays a variety of quotations, especially visual ones. His 2001 Je suis sang (conte de fées médiéval is directly and indirectly inspired by Saint Sebastian’s iconography. This study examines the modalities and functions of this re-use in detail.

FLORIDA HAZARDOUS WASTE AND SANITARY LANDFILL REPORT, COUNTY DATA. GENERATOR DATA AND CHARACTERISTICS OF SANITARY LANDFILLS. PART 2. COUNTIES: BROWARD, CALHOUN, CHARLOTTE, CITRUS, CLAY, COLLIER

Science.gov (United States)

The report provides data on the use of sanitary landfills (Subtitle D facilities) for hazardous waste disposal in Florida by small quantity generators. It consists of eleven parts including a part called Study Area Data which contains the data aggregated across the counties cover...

[Sanitary and epidemiological supply for the Russian Army during the First World War (1914-1918)].

Science.gov (United States)

gorelova, L E; Loktev, A E

2014-02-01

At the beginning of the First World War the most typical diseases in the Russian Army were typhoid, typhus, diphtheria, cholera, smallpox and other infectious diseases. At the beginning of the First World War the level of infectious morbidity was significantly low, but further increased and pandemic risk arose. Servicemen were mostly ill with typhus, relapsing fever, flux, cholera, smallpox and typhoid. The highest mortality rate was registered in patients with cholera, typhus and typhoid. According the prewar deployment program of the Russian Army anti-epidemiologic facilities were established. By the end of war were established 110 sanitary-and-hygienic and 90 disinfection units. However, organization of anti-epidemiologic security was unsatisfactory. Due to lack of specialists and equipment anti-epidemiologic facilities of units were under strength. Commanders of sanitary units and sanitary service had not enough resources for operational service in the Forces and facilities of rear area.

Detection of Multiple Parallel Transmission Outbreak of Streptococcus suis Human Infection by Use of Genome Epidemiology, China, 2005.

Science.gov (United States)

Du, Pengcheng; Zheng, Han; Zhou, Jieping; Lan, Ruiting; Ye, Changyun; Jing, Huaiqi; Jin, Dong; Cui, Zhigang; Bai, Xuemei; Liang, Jianming; Liu, Jiantao; Xu, Lei; Zhang, Wen; Chen, Chen; Xu, Jianguo

2017-02-01

Streptococcus suis sequence type 7 emerged and caused 2 of the largest human infection outbreaks in China in 1998 and 2005. To determine the major risk factors and source of the infections, we analyzed whole genomes of 95 outbreak-associated isolates, identified 160 single nucleotide polymorphisms, and classified them into 6 clades. Molecular clock analysis revealed that clade 1 (responsible for the 1998 outbreak) emerged in October 1997. Clades 2-6 (responsible for the 2005 outbreak) emerged separately during February 2002-August 2004. A total of 41 lineages of S. suis emerged by the end of 2004 and rapidly expanded to 68 genome types through single base mutations when the outbreak occurred in June 2005. We identified 32 identical isolates and classified them into 8 groups, which were distributed in a large geographic area with no transmission link. These findings suggest that persons were infected in parallel in respective geographic sites.

Characterization of a Streptococcus suis tet(O/W/32/O)-carrying element transferable to major streptococcal pathogens.

Science.gov (United States)

Palmieri, Claudio; Magi, Gloria; Mingoia, Marina; Bagnarelli, Patrizia; Ripa, Sandro; Varaldo, Pietro E; Facinelli, Bruna

2012-09-01

Mosaic tetracycline resistance determinants are a recently discovered class of hybrids of ribosomal protection tet genes. They may show different patterns of mosaicism, but their final size has remained unaltered. Initially thought to be confined to a small group of anaerobic bacteria, mosaic tet genes were then found to be widespread. In the genus Streptococcus, a mosaic tet gene [tet(O/W/32/O)] was first discovered in Streptococcus suis, an emerging drug-resistant pig and human pathogen. In this study, we report the molecular characterization of a tet(O/W/32/O) gene-carrying mobile element from an S. suis isolate. tet(O/W/32/O) was detected, in tandem with tet(40), in a circular 14,741-bp genetic element (39.1% G+C; 17 open reading frames [ORFs] identified). The novel element, which we designated 15K, also carried the macrolide resistance determinant erm(B) and an aminoglycoside resistance four-gene cluster including aadE (streptomycin) and aphA (kanamycin). 15K appeared to be an unstable genetic element that, in the absence of recombinases, is capable of undergoing spontaneous excision under standard growth conditions. In the integrated form, 15K was found inside a 54,879-bp integrative and conjugative element (ICE) (50.5% G+C; 55 ORFs), which we designated ICESsu32457. An ∼1.3-kb segment that apparently served as the att site for excision of the unstable 15K element was identified. The novel ICE was transferable at high frequency to recipients from pathogenic Streptococcus species (S. suis, Streptococcus pyogenes, Streptococcus pneumoniae, and Streptococcus agalactiae), suggesting that the multiresistance 15K element can successfully spread within streptococcal populations.

[Sanitary and epidemiological conditions in the Lodz region during the period of the second Polish Republic in the XIX and XX centuries].

Science.gov (United States)

Sadowska, Jolanta

2005-01-01

Throughout history of European agglomerations in the nineteenth and twentieth century the relation between expanding industry and worsening sanitary and epidemiological conditions was seen. These changes, having great impact on public health, were investigated in the present study. Problems related to sanitary and epidemiological conditions in Lodz first emerged during the Russian occupation of Poland in the XIX century. The Russian Governing Council was not overseeing sanitary conditions of the city, and transferred this responsibility to the industrial bourgeoisie of Lodz. In this situation, uncontrolled development of the industry led to serious deterioration in natural environment and increase of risk for acute and chronic infectious diseases. Improvement of the sanitary situation in Lodz commenced in the period of the Second Polish Republic (1918-1939). Sanitary Divisions were created at the Lodz Department of Public Health. Activities in this Department involved, among others, detailed epidemiological examination of potable water, the investigation of manufacturing processes, retail and wholesale groceries, hygienic conditions of public housing. The activity and interventions of Sanitary Divisions contributed substantially to a decrease of incidence of various diseases and improvement of living conditions of the Lodz populations. The improvement of sanitary and epidemiological conditions in Lodz after 1918 was mainly related to the construction of water supply and sewage systems. This led to an approximation of the urban standards of this 500,000 population agglomeration to European standards.

[V.P. Kravkov as a sanitary physician of the Russian Imperial Army (to centenary of beginning of the First World War)].

Science.gov (United States)

Uzbekova, D G

2014-01-01

The article presents for the first time the description of life story and service of V.P. Kravkov (1859-1920)--participant of Russian-Japanese war and the First World War, doctor of medicine, sanitary physician of the Russian Imperial Army and author of number of books and articles on preventive medicine. During these two wars, V.P. Kravkov organized sanitary epidemiological service in fronts. He kept diary regularly reflecting conditions of sanitary epidemiological service during this period, describing one's own observations and assessing survived events. The manuscripts of diaries are stored in the Russian state library. The diaries made great input into summarizing of experience of the Russian military sanitary service and became valuable monument of military memoirs' literature.

Diptera of sanitary importance associated with composting of biosolids in Argentina

Directory of Open Access Journals (Sweden)

Valeria Alejandra Labud

2003-12-01

Full Text Available OBJECTIVE: Odorous compounds produced at the biosolids composting plant in Bariloche (NW Patagonia attract a variety of insects, mainly belonging to the order Diptera. In order to characterize these flies, collected specimens were taxonomically identified, their community characteristics were described and their sanitary and synanthropic importance and autochthonous or introduced character were determined. METHODS: Sampling was performed from October 1999 until March 2000. Adults were collected using an entomological net, and larvae and puparia were obtained from the composting material and incubated to obtain adults. Richness, abundance and sex ratio were calculated. RESULTS: A total of 9 taxa of Diptera were identified: Sarconesia chlorogaster, Phaenicia sericata, Calliphora vicina, Cochliomya macellaria, Ophyra sp, Muscina stabulans, Musca domestica, Sarcophaga sp and Fannia sp. Specimens of Anthomyiidae, Acaliptratae and one larva of Eristalis tenax were also found. Ophyra sp. was the most abundant taxa. All the captured Diptera belonged to introduced taxa. Most of them are considered to be eusynanthropic and/or hemisynanthropic and have sanitary importance as they may cause myiasis and pseudomyiasis. The high number of females registered and the finding of immature stages indicated that flies can develop their complete life cycle on biosolid composting windrows. CONCLUSIONS: The characterization of flies obtained in this study may be useful for defining locations of urban or semi-urban composting facilities. It also highlights the importance of sanitary precautions at such plants.

Characterization of virulence of the Streptococcus suis serotype 2 reference strain Henrichsen S 735 in newborn gnotobiotic pigs

NARCIS (Netherlands)

Vecht, U.; Wisselink, H.J.; Stockhofe-Zurwieden, N.; Smith, H.E.

1996-01-01

Strain Henrichsen S 735 (NCTC 10234) of Streptococcus suis serotype 2 reference and three other such strains (strains S 4005, S 3921 and T 141) were tested for virulence by inoculating pigs intranasally and intravenously. The taxonomical properties of each strain were determined. Phenotypes were

COMPUTER MODEL ANALYSIS FOR MITIGATION PLANNING OF SANITARY-SEWER OVERFLOWS

Science.gov (United States)

Sanitary sewer overflows (SSOs) are generally difficult to witness or document as they usually occur during rain events when people are indoors or out of sight. To anser where and when an SSO may occur, it is necessary to know the flow conveyance capacity at various parts of the ...

Determination of the gaseous emission of toxic substances in the Curva de Rodas sanitary landfill in Medellin

International Nuclear Information System (INIS)

Garcia Renteria, Francisco Fernando; Agudelo Garcia, Ruben Alberto

2005-01-01

Results of the investigation conducted at the sanitary landfill Curva de Rodas, aimed to determine the emission and migration of toxic substances are presented. Traces of benzene, toluene, hexane, vinyl chloride and xylene were found. Concentrations of these substances were, however, below threshold limits at the landfill and below detectable limits in the air of populated areas adjacent to the sanitary landfill

Book review. Gli animali, l'uomo e l'ambiente. (a cura di Giorgio Battelli, Raffaella Baldelli, Fabio Ostanello, Santino Prosperi

Directory of Open Access Journals (Sweden)

Manuel Graziani

2013-12-01

Full Text Available Da sempre i Servizi Veterinari Pubblici sono una componente poco conosciuta del Servizio Sanitario Nazionale, le loro prestazioni vengono spesso fruite in modo inconsapevole sia dalla popolazione che dall'amministrazione pubblica. È pleonastico ricordare che si tratta di attività sanitarie il cui scopo prevalente è quello di tutelare la salute animale e umana e di promuovere la produttività e il benessere delle popolazioni animali. Ma vale la pena ribadire che tali attività hanno un ruolo assai rilevante anche sotto il profilo economico e sociale. Questo corposo volume fa il punto sulle competenze che le diverse componenti professionali devono possedere. Un volume importante per comprendere la realtà veterinaria italiana, pubblica e privata, nato dal lavoro congiunto di figure di primo livello della medicina veterinaria del Paese: i curatori sono tutti docenti del Dipartimento di Scienze Mediche Veterinarie dell'Università di Bologna, convinti assertori del ruolo sociale della Medicina veterinaria, del concetto di Medicina/Salute unica e della necessità di migliorare l'aspetto formativo. I professori Battelli, Baldelli, Ostanello e Prosperi fanno parte di quella che viene definita la "Scuola bolognese di Sanità Pubblica Veterinaria" il cui fondatore è stato Adriano Mantovani (scomparso a marzo dello scorso anno, unanimemente considerato il Padre della SPV italiana e di cui vengono qui pubblicati 3 scritti e una breve scheda biografica che lo ricorda. Il volume è suddiviso in tre parti allo scopo di fornire al lettore un percorso interpretativo logico che si sviluppa verso la comprensione delle competenze e dei campi di attività dei Servizi Veterinari nel nostro Paese, fino a mettere in luce le sfide attuali e future che la SPV deve affrontare. Particolare attenzione viene posta nei confronti dell'evoluzione che tali attività sanitarie potranno avere nel prossimo futuro, in riferimento ai cambiamenti socio-economici, alle attivit

Sanitary hot water; Eau chaude sanitaire

Energy Technology Data Exchange (ETDEWEB)

NONE

2004-07-01

Cegibat, the information-recommendation agency of Gaz de France for building engineering professionals, has organized this conference meeting on sanitary hot water to present the solutions proposed by Gaz de France to meet its clients requirements in terms of water quality, comfort, energy conservation and respect of the environment: quantitative aspects of the hot water needs, qualitative aspects, presentation of the Dolce Vita offer for residential buildings, gas water heaters and boilers, combined solar-thermal/natural gas solutions, key-specifications of hot water distribution systems, testimony: implementation of a gas hot water reservoir and two accumulation boilers in an apartment building for young workers. (J.S.)

Ecotoxicological evaluation of leachate from the Limeira sanitary landfill with a view to identifying acute toxicity

OpenAIRE

José Euclides Stipp Paterniani; Ronaldo Teixeira Pelegrini; Núbia Natália de Brito Pelegrini

2007-01-01

Final disposal of solid waste is still a cause for serious impacts on the environment. In sanitary landfills, waste undergoes physical, chemical, and biological decomposition, generating biogas and leachate. Leachate is a highly toxic liquid with a very high pollution potential. The purpose of this work is to evaluate toxicity of in natura leachate samples collected from Limeira Sanitary Landfill, in Limeira, SP. The ecotoxicological evaluation comprised acute toxicity assays using as test or...

Florence Nightingale and the India sanitary reforms.

Science.gov (United States)

Hays, J C

1989-09-01

After the Crimean War, Florence Nightingale persisted in researching the health conditions of British troops throughout the Empire. Undaunted by geographic limitations, she surveyed and publicized data that documented the mismanagement of living conditions and health care among the occupational forces on the Indian continent. Nightingale proposed widespread changes in the reporting of military health status and biostatistics, in sanitary engineering, and in self-care activities. With dogged persistence, she continued to gather follow-up data to measure the changing health status of soldiers in a land she never saw.

Prevalence and potentially reversible factors associated with anorexia among older nursing home residents: results from the ULISSE project.

Science.gov (United States)

Landi, Francesco; Lattanzio, Fabrizia; Dell'Aquila, Giuseppina; Eusebi, Paolo; Gasperini, Beatrice; Liperoti, Rosa; Belluigi, Andrea; Bernabei, Roberto; Cherubini, Antonio

2013-02-01

The principal aims of the present study were to explore the prevalence of anorexia and the factors correlated to anorexia in a large population of older people living in nursing home. Secondary, we evaluated the impact of anorexia on 1-year survival. Data are from baseline evaluation of 1904 participants enrolled in the Un Link Informatico sui Servizi Sanitari Esistenti per l'Anziano study, a project evaluating the quality of care for older persons living in an Italian nursing home. All participants underwent a standardized comprehensive evaluation using the Italian version of the inter Resident Assessment Instrument Minimum Data Set (version 2.0) for Nursing Home. We defined anorexia as the presence of lower food intake. The relationship between covariates and anorexia was estimated by deriving ORs and relative 95% CIs from multiple logistic regression models including anorexia as the dependent variable of interest. Hazard ratios and 95% CIs for mortality by anorexia were calculated. More than 12% (240 participants) of the study sample suffered from anorexia, as defined by the presence of decreased food intake or the presence of poor appetite. Participants with functional impairment, dementia, behavior problems, chewing problems, renal failure, constipation, and depression, those treated with proton pump inhibitors and opioids had a nearly 2-fold increased risk of anorexia compared with participants not affected by these syndromes. Furthermore, participants with anorexia had a higher risk of death for all causes compared with nonanorexic participants (hazard ratio 2.26, 95% CI: 2.14-2.38). The major finding is that potentially reversible causes, such as depression, pharmacologic therapies, and chewing problems, were strongly and independently associated with anorexia among frail older people living in nursing home. Furthermore, anorexia was associated with higher rate of mortality, independently of age and other clinical and functional variables. Copyright © 2013

Sanitary surveillance and bioethics

Directory of Open Access Journals (Sweden)

Volnei Garrafa

2017-08-01

Full Text Available Regulatory practices in the field of health surveillance are indispensable. The aim of this study is to show ‒ taking the Brazilian National Surveillance Agency, governing body of sanitary surveillance in Brazil as a reference ‒ that bioethics provides public bodies a series of theoretical tools from the field of applied ethics for the proper exercise and control of these practices. To that end, the work uses two references of bioethics for the development of a comparative and supportive analysis to regulatory activities in the field of health surveillance: the Universal Declaration on Bioethics and Human Rights of Unesco and the theory of intervention bioethics. We conclude that organizations and staff working with regulatory activities can take advantage of the principles and frameworks proposed by bioethics, especially those related to the Declaration and the theory of intervention bioethics, the latter being set by the observation and use of the principles of prudence, precaution, protection and prevention.

Interim sanitary landfill groundwater monitoring report. 1996 Annual report

International Nuclear Information System (INIS)

Bagwell, L.A.

1997-01-01

Eight wells of the LFW series monitor groundwater quality in the Steed Pond Aquifer (Water Table) beneath the Interim Sanitary Landfill at the Savannah River Site. These wells are sampled semiannually to comply with the South Carolina Department of Health and Environmental Control Modified Municipal Solid Waste Permit 025500-1120 and as part of the SRS Groundwater Monitoring Program. Trichlorofluoromethane and 1,1,1-trichloroethane were elevated in one sidegradient well and one downgradient well during 1996. Zinc was elevated in three downgradient wells and also was detected in the associated laboratory blanks for two of those wells. Specific conductance was elevated in one background well and one sidegradient well. Barium and copper exceeded standards in one sidegradient well, and dichloromethane (a common laboratory contaminant) was elevated in another sidegradient well. Barium, copper, and dichloromethane were detected in the associated blanks for these wells, also. The groundwater flow direction in the Steed Pond Acquifer (Water Table) beneath the Interim Sanitary Landfill was to the southeast (universal transverse Mercator coordinates). The flow rate in this unit was approximately 210 ft/year during first quarter 1996 and 180 ft/yr during third quarter 1996

First insights into the protective effects of a recombinant swinepox virus expressing truncated MRP of Streptococcus suis type 2 in mice.

Science.gov (United States)

Huang, Dongyan; Zhu, Haodan; Lin, Huixing; Xu, Jiarong; Lu, Chengping

2012-01-01

To explore the potential of the swinepox virus (SPV) as vector for Streptococcus suis vaccines, a vector system was developed for the construction of a recombinant SPV carrying bacterial genes. Using this system, a recombinant virus expressing truncated muramidase-released protein (MRP) of S. suis type 2 (SS2), designated rSPV-MRP, was produced and identified by PCR, western blotting and immunofluorescence assays. The rSPV-MRP was found to be only slightly attenuated in PK-15 cells, when compared with the wild-type virus. After immunization intramuscularly with rSPV-MRP, SS2 inactive vaccine (positive control), wild-type SPV (negative control) and PBS (blank control) respectively, all CD1 mice were challenged with a lethal dose or a sublethal dose of SS2 highly virulent strain ZY05719. While SS2 inactive vaccine protected all mice, immunization with rSPV-MRP resulted in 60% survival and protected mice against a lethal dose of the highly virulent SS2 strain, compared with the negative control (P MRP had a significantly reduced bacterial burden in all organs examined, compared to negative controls and blank controls (P MRP-vaccinated group were significantly higher (P MRP provided mice with protection from systemic SS2 infection. If SPV recombinants have the potential as S. suis vaccines for the use in pigs has to be evaluated in further studies.

Concentração inibitória mínima (CIM de oito antimicrobianos frente isolados de Streptococcus suis

Directory of Open Access Journals (Sweden)

Felipe Masiero Salvarani

2008-02-01

Full Text Available The minimun inhibitory concentration (MIC was determined toward amoxicilin, ampicilin, penicillin, ceftiofur, florfenicol, lincomycin, trimethoprim-sulfadiazine and tetracycline for 75 strains of Streptococcus suis. The MIC was performed on sheep blood agar plates containing concentrations varying from 0,25 to 256 µg/ml of the antibiotic described above. The amoxicilin in the concentrations of 1 and 2 µg/ml, florfenicol in the concentration of 1 µg/ml and trimethoprim-sulfadiazine in the concentrations of 2 e 8 µg/ml, were the antibiotics that presented minor resistance. In contrast, the ampicilin, tetracycline, ceftiofur and lincomycin, presented MIC of 64 and 128 µg/ml, 64 and 128 µg/ml, 128 and 256 µg/ml and >;256 µg/ml, respectively. The results of this study show that the amoxicilin and florfenicol are the antibiotics of choice for the treatment of diseases by S. suis in swine.

Sanitary landfill groundwater monitoring report. Fourth quarter 1994 and 1994 summary

International Nuclear Information System (INIS)

1995-02-01

Eighty-nine wells of the LFW series monitor groundwater quality in the Steed Pond Aquifer (Water Table) beneath the Sanitary Landfill at the Savannah River Site (SRS). These wells are sampled quarterly to comply with the South Carolina Department of Health and Environmental Control Domestic Waste Permit DWP-087A and as part of the SRS Groundwater Monitoring Program. Dichloromethane, a common laboratory contaminant, and trichloroethylene were the most widespread constituents exceeding standards during 1994. Benzene, chloroethene (vinyl chloride), 1,2-dichloroethane, 1,1-dichloroethylene, 1,2-dichloropropane, gross alpha, mercury, nonvolatile beta, tetrachloroethylene, and tritium also exceeded standards in one or more wells. The groundwater flow direction in the Steed Pond Aquifer (Water Table) beneath the Sanitary Landfill was to the southeast (universal transverse Mercator coordinates). The flow rate in this unit was approximately 140 ft/year during first and fourth quarters 1994

Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis

Directory of Open Access Journals (Sweden)

Dana Ditgen

2016-01-01

Full Text Available The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S products of Strongyloides ratti and Trichuris suis sequences for thioredoxin (Trx and Trx-like protein (Trx-lp were identified. To characterize the antioxidant Trx-lp and its interaction with the parasite’s mucosal habitat, S. ratti and T. suis Trx-lps were cloned and recombinantly expressed. The primary antioxidative activity was assured by reduction of insulin and IgM. Further analysis applying an in vitro mucosal 3D-cell culture model revealed that the secreted Trx-lps were able to bind to monocytic and intestinal epithelial cells and induce the time-dependent release of cytokines such as TNF-α, IL-22, and TSLP. In addition, the redox proteins also possessed chemotactic activity for monocytic THP-1 cells and fostered epithelial wound healing activity. These results confirm that the parasite-secreted Trx-lps are multifunctional proteins that can affect the host intestinal mucosa.

Interim Sanitary Landfill Groundwater Monitoring Report. 1997 Annual Report

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-01-01

Eight wells of the LFW series monitor groundwater quality in the Steed Pond Aquifer (Water Table) beneath the Interim Sanitary Landfill at the Savannah River Site (SRS). These wells are sampled semiannually to comply with the South Carolina Department of Health and Environmental Control Modified Municipal Solid Waste Permit 025500-1120 (formerly dWP-087A) and as part of the SRS Groundwater Monitoring Program.

Sanitary and hygienic state estimation of population determined by cancer morbidity level

International Nuclear Information System (INIS)

Coretchi, L.; Cucereanu, A.

2009-01-01

The European recommendations of Cancer Register elaboration are presented in this paper. A short literature review about sanitary and hygienic estimation status of population thru determination of cancer morbidity level also has been performed. (authors)

RegA Plays a Key Role in Oxygen-Dependent Establishment of Persistence and in Isocitrate Lyase Activity, a Critical Determinant of In vivo Brucella suis Pathogenicity

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Elias Abdou

2017-05-01

Full Text Available For aerobic human pathogens, adaptation to hypoxia is a critical factor for the establishment of persistent infections, as oxygen availability is low inside the host. The two-component system RegB/A of Brucella suis plays a central role in the control of respiratory systems adapted to oxygen deficiency, and in persistence in vivo. Using an original “in vitro model of persistence” consisting in gradual oxygen depletion, we compared transcriptomes and proteomes of wild-type and ΔregA strains to identify the RegA-regulon potentially involved in the set-up of persistence. Consecutive to oxygen consumption resulting in growth arrest, 12% of the genes in B. suis were potentially controlled directly or indirectly by RegA, among which numerous transcriptional regulators were up-regulated. In contrast, genes or proteins involved in envelope biogenesis and in cellular division were repressed, suggesting a possible role for RegA in the set-up of a non-proliferative persistence state. Importantly, the greatest number of the RegA-repressed genes and proteins, including aceA encoding the functional IsoCitrate Lyase (ICL, were involved in energy production. A potential consequence of this RegA impact may be the slowing-down of the central metabolism as B. suis progressively enters into persistence. Moreover, ICL is an essential determinant of pathogenesis and long-term interactions with the host, as demonstrated by the strict dependence of B. suis on ICL activity for multiplication and persistence during in vivo infection. RegA regulates gene or protein expression of all functional groups, which is why RegA is a key regulator of B. suis in adaptation to oxygen depletion. This function may contribute to the constraint of bacterial growth, typical of chronic infection. Oxygen-dependent activation of two-component systems that control persistence regulons, shared by several aerobic human pathogens, has not been studied in Brucella sp. before. This work

Hygienic-sanitary profile and microbiological dangers in public slaughterhouses

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Edinaidy Suianny Rocha de Moura

2015-10-01

Full Text Available ABSTRACT. Moura E.S.R., Abrantes M.R., Mendes C.G., Oliveira A.R.M, Souza E.S. & Silva J.B.A. [Hygienic-sanitary profile and microbiological dangers in public slaughterhouses.] Perfil higiênico-sanitário e perigos microbiológicos em abatedouros públicos. Revista Brasileira de Medicina Veterinária, 37(3:203-208, 2015. Departamento de Ciência Animal, Universidade Federal Rural do Semi-Árido, Avenida Francisco Mota, 572, Bairro Costa e Silva, Mossoró, RN 59625-900, Brasil. E-mail: jeanberg@ufersa.edu.br The aim of this study was to evaluate hygienic and sanitary conditions of municipal slaughterhouses in Rio Grande do Norte. Through a check list, hygienic conditions of establishments and handlers were observed and the percentage of compliance was rated excellent (≥ 80%, good (60 to 79.9%, regular (40 to 59.9% and poor (<40%, according to official standards established by Brazilian legislation. Ten water samples were collected to analyze total and thermotolerant coliforms; and 200 swab samples, including 150 from the utensils, equipment, handlers, and cattle carcasses were investigated for Staphylococcus aureus and Enterobacteria, and 50 from the environment for Listeria sp. research. It was noted, through the check list, the existence of various inadequate parameters when compared to those established, and required by law. According to the percentage of compliance found in abattoirs during the assessment visits, no slaughterhouse was rated as excellent, only one was considered good, one was classified as regular and three were considered poor. As for the microbiological analyzes, the presence of coliforms was confirmed in water from two slaughterhouses. High bacteria counts were found in swabs from tools, handlers, and cattle carcasses, and the presence of Listeria sp. was observed in three slaughterhouses. Therefore, the precarious sanitary conditions of the municipal slaughterhouses studied in Rio Grande do Norte represent a risk to

Changes in abundance of Lactobacillus spp. and Streptococcus suis in the stomach, jejunum and ileum of piglets after weaning

NARCIS (Netherlands)

Su, Y.; Yao, W.; Perez-Gutierrez, O.N.; Smidt, H.; Zhu, W.Y.

2008-01-01

This present study investigated the changes in bacterial community composition, with an emphasis on Lactobacillus spp. and Streptococcus suis populations as potentially beneficial and harmful groups, in the stomach, jejunum and ileum of piglets after weaning (21 days postpartum) by 16S rRNA

Detection of a quantitative trait locus associated with resistance to infection with Trichuris suis in pigs

DEFF Research Database (Denmark)

Skallerup, Per; Thamsborg, Stig Milan; Jørgensen, Claus Bøttcher

2015-01-01

diarrhoea. A significant proportion of the variation in Trichuris faecal egg count (FEC) has been attributed to the host's genetic make-up. The aim of the present study was to identify genetic loci associated with resistance to T. suis in pigs. We used single nucleotide polymorphism (SNP) markers to perform...

[The senses of sanitary safety in the discourse of the National Health Surveillance Agency].

Science.gov (United States)

Barbosa, Ana de Oliveira; Costa, Ediná Alves

2010-11-01

The term sanitary safety (SS) appeared in the international debate mainly due to the emerging sanitary crisis, although its meaning has remained obscure. This paper aims to analyze the concept of SS brought into the Brazilian sanitary surveillance upon the creation of the National Health Surveillance Agency. An exploratory case study was undertaken with technical data analysis and semi-structured interviews with informants who had taken part in the process of formulating the body's institutional design. The following categories were analyzed: incorporation of the SS term into the institutional mission, the SS concept and SS mechanisms. The SS concept was analyzed in both institutional and technical discursive dimensions. The former elicits the sense of strategy, a reliable relationship and legitimacy whereas the latter shows the sense of an acceptable risk-benefit relationship from the perspective of individual and collective health protection and promotion. The SS concept was found to encompass health-related products, technologies and services, especially those designed for medical diagnosis and treatment, but environmental issues received little mention. The scope of the SS concept was shown to be widening to include the surveillance of hospital infection, drugs and blood.

RESULTS AND PROSPECTS OF SANITARY AND EPIDEMIOLOGICAL WELL-BEING ENSURING FOR THE POPULATION OF RUSSIAN FEDERATION

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G. G. Onischenko

2008-01-01

Full Text Available The report states priority goals of sanitary and epidemiological service and shows the analysis of the Service's administrative reforms and structural changes from 1991 to 2007. It considers the existing regulatory and legal background on all aspects of hygiene and epidemiology as well as the Concept of the Federal Service Development. It analyses the work of Rospotrebnadzor  bodies in ensuring of sanitary and epidemiological well-being of the population, including current state of population radiation protection in Russian Federation.

NORTH PORTAL - SANITARY SEWER CALCULATION - CHANGE HOUSE FACILITY No.5008

International Nuclear Information System (INIS)

Blackstone, R.

1996-01-01

The purpose of this design calculation is to determine the demand on the waste system and to size the three main sanitary sewer lines serving the Change House Facility No.5008, in accordance with the Uniform Plumbing Code (Section 4.4.1) and U.S. Department of Energy Order 6430.1A-1540 (Section 4.4.2)

The health of hospitals and lessons from history: public health and sanitary reform in the Dublin hospitals, 1858-1898.

Science.gov (United States)

Fealy, Gerard M; McNamara, Martin S; Geraghty, Ruth

2010-12-01

The aim was to examine, critically, 19th century hospital sanitary reform with reference to theories about infection and contagion. In the nineteenth century, measures to control epidemic diseases focused on providing clean water, removing waste and isolating infected cases. These measures were informed by the ideas of sanitary reformers like Chadwick and Nightingale, and hospitals were an important element of sanitary reform. Informed by the paradigmatic tradition of social history, the study design was a historical analysis of public health policy. Using the methods of historical research, documentary primary sources, including official reports and selected hospital archives and related secondary sources, were consulted. Emerging theories about infection were informing official bodies like the Board of Superintendence of Dublin Hospitals in their efforts to improve hospital sanitation. The Board secured important reforms in hospital sanitation, including the provision of technically efficient sanitary infrastructure. Public health measures to control epidemic infections are only as effective as the state of knowledge of infection and contagion and the infrastructure to support sanitary measures. Today, public mistrust about the safety of hospitals is reminiscent of that of 150 years ago, although the reasons are different and relate to a fear of contracting antimicrobial-resistant infections. A powerful historical lesson from this study is that resistance to new ideas can delay progress and improved sanitary standards can allay public mistrust. In reforming hospital sanitation, policies and regulations were established--including an inspection body to monitor and enforce standards--the benefits of which provide lessons that resonate today. Such practices, especially effective independent inspection, could be adapted for present-day contexts and re-instigated where they do not exist. History has much to offer contemporary policy development and practice reform and

Law no. 2001-398 of the 9 may 2001 creating a french Agency of sanitary and environmental safety (1)

International Nuclear Information System (INIS)

2001-05-01

This law text indicates the goal and the missions of the french sanitary and environmental safety Agency, defined by the law no.2001-398: to assure the public health the Agency has to contribute to the the sanitary safety in the environment domain and to evaluate the risk bonded to the environment. (A.L.B)

The Fast-Growing Brucella suis Biovar 5 Depends on Phosphoenolpyruvate Carboxykinase and Pyruvate Phosphate Dikinase but Not on Fbp and GlpX Fructose-1,6-Bisphosphatases or Isocitrate Lyase for Full Virulence in Laboratory Models

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Amaia Zúñiga-Ripa

2018-04-01

Full Text Available Bacteria of the genus Brucella infect a range of vertebrates causing a worldwide extended zoonosis. The best-characterized brucellae infect domestic livestock, behaving as stealthy facultative intracellular parasites. This stealthiness depends on envelope molecules with reduced pathogen-associated molecular patterns, as revealed by the low lethality and ability to persist in mice of these bacteria. Infected cells are often engorged with brucellae without signs of distress, suggesting that stealthiness could also reflect an adaptation of the parasite metabolism to use local nutrients without harming the cell. To investigate this, we compared key metabolic abilities of Brucella abortus 2308 Wisconsin (2308W, a cattle biovar 1 virulent strain, and B. suis 513, the reference strain of the ancestral biovar 5 found in wild rodents. B. suis 513 used a larger number of C substrates and showed faster growth rates in vitro, two features similar to those of B. microti, a species phylogenomically close to B. suis biovar 5 that infects voles. However, whereas B. microti shows enhanced lethality and reduced persistence in mice, B. suis 513 was similar to B. abortus 2308W in this regard. Mutant analyses showed that B. suis 513 and B. abortus 2308W were similar in that both depend on phosphoenolpyruvate synthesis for virulence but not on the classical gluconeogenic fructose-1,6-bisphosphatases Fbp-GlpX or on isocitrate lyase (AceA. However, B. suis 513 used pyruvate phosphate dikinase (PpdK and phosphoenolpyruvate carboxykinase (PckA for phosphoenolpyruvate synthesis in vitro while B. abortus 2308W used only PpdK. Moreover, whereas PpdK dysfunction causes attenuation of B. abortus 2308W in mice, in B. suis, 513 attenuation occurred only in the double PckA-PpdK mutant. Also contrary to what occurs in B. abortus 2308, a B. suis 513 malic enzyme (Mae mutant was not attenuated, and this independence of Mae and the role of PpdK was confirmed by the lack of

Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2.

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Jiaqi Tang

2006-05-01

Full Text Available BACKGROUND: Streptococcus suis serotype 2 (S. suis 2, SS2 is a major zoonotic pathogen that causes only sporadic cases of meningitis and sepsis in humans. Most if not all cases of Streptococcal toxic shock syndrome (STSS that have been well-documented to date were associated with the non-SS2 group A streptococcus (GAS. However, a recent large-scale outbreak of SS2 in Sichuan Province, China, appeared to be caused by more invasive deep-tissue infection with STSS, characterized by acute high fever, vascular collapse, hypotension, shock, and multiple organ failure. METHODS AND FINDINGS: We investigated this outbreak of SS2 infections in both human and pigs, which took place from July to August, 2005, through clinical observation and laboratory experiments. Clinical and pathological characterization of the human patients revealed the hallmarks of typical STSS, which to date had only been associated with GAS infection. Retrospectively, we found that this outbreak was very similar to an earlier outbreak in Jiangsu Province, China, in 1998. We isolated and analyzed 37 bacterial strains from human specimens and eight from pig specimens of the recent outbreak, as well as three human isolates and two pig isolates from the 1998 outbreak we had kept in our laboratory. The bacterial isolates were examined using light microscopy observation, pig infection experiments, multiplex-PCR assay, as well as restriction fragment length polymorphisms (RFLP and multiple sequence alignment analyses. Multiple lines of evidence confirmed that highly virulent strains of SS2 were the causative agents of both outbreaks. CONCLUSIONS: We report, to our knowledge for the first time, two outbreaks of STSS caused by SS2, a non-GAS streptococcus. The 2005 outbreak was associated with 38 deaths out of 204 documented human cases; the 1998 outbreak with 14 deaths out of 25 reported human cases. Most of the fatal cases were characterized by STSS; some of them by meningitis or severe

Sanitary hot water consumption patterns in commercial and industrial sectors in South Africa: Impact on heating system design

International Nuclear Information System (INIS)

Rankin, R.; Rousseau, P.G.

2006-01-01

A large amount of individual sanitary hot water consumers are present in the South African residential sector. This led to several studies being done on hot water consumption patterns in this sector. Large amounts of sanitary hot water are also consumed in the commercial sector in buildings such as hotels and in large residences such as those found in the mining industry. The daily profiles of sanitary hot water consumption are not related to any technical process but rather to human behavior and varying ambient conditions. The consumption of sanitary hot water, therefore, represents a challenge to the electrical utility because it is an energy demand that remains one of the biggest contributors to the undesirable high morning and afternoon peaks imposed on the national electricity supply grid. It also represents a challenge to sanitary hot water system designers because the amount of hot water as well as the daily profile in which it is consumed impacts significantly on system design. This paper deals with hot water consumption in the commercial and industrial sectors. In the commercial sector, we look at hotels and in the industrial sector at large mining residences. Both of them are served by centralized hot water systems. Measured results from the systems are compared to data obtained from previous publications. A comparison is also made to illustrate the impact that these differences will have on sanitary hot water system design. Simulations are conducted for these systems using a simulation program developed in previous studies. The results clearly show significant differences in the required heating and storage capacity for the new profiles. A twin peak profile obtained from previous studies in the residential sector was used up to now in studies of heating demand and system design in commercial buildings. The results shown here illustrate the sanitary hot water consumption profile differs significantly from the twin peaks profile with a very high morning

Design of manuals sanitary landfills (II). Diseo de rellenos sanitarios manuales (II)

Energy Technology Data Exchange (ETDEWEB)

Jaramillo, J.

1994-01-01

This period includes engineering projects, landscape and details of building; taking into account the circumstances that govern them, it must be made looking at the simplicity and quickness required to these type of works and trying to respect sanitary requirements. (Author)

The sanitary effect of gamma irradiation on sewage sludge

International Nuclear Information System (INIS)

Hess, E.; Breer, C.

1975-01-01

Sludge contains Salmonellae in more than 90% of samples. The maximum number reaches 10 7 per liter. Neither aerobic stabilization nor anaerobic digestion significantly reduces the contamination with Salmonellae. Moreover, Salmonellae in sewage sludge spread on grass may survive up to 72 weeks. Fertilizing with unsanitized sludge may therefore lead to transmission from plant to animal. Sanitizing of sludge to be used as fertilizer is therefore urgent. The sanitary effect of pasteurisation and of gamma irradiation on sewage sludge was investigated. For this the number of Enterobacteriaceae before and after irradiation in 259 specimens of sludge from 44 different sewage disposal plants was examined. The doses applied were 100, 200, 300, 400 and sometimes 500 krad. A linear reduction of Enterobacteriaceae was achieved with increasing radiation doses. A dose of 300 krad resulted in a death rate of 10 4 - 10 8 , occasionally 10 9 Enterobacteriaceae. Less than 10 Enterobacteriaceae per gramm were found in 97.2% of the samples irradiated with 300 krad. The effect found in the above mentioned model experiments could be perfectly confirmed under practical conditions in the irradiation plant of Geiselbullach. The sanitary effect of gamma irradiation with 300-350 krad, determined by Enterobacteriaceae reduction, was equivalent to the effect of heat treatment by pasteurisation. (orig./MG) [de

MATERIALS FOR THE FINAL COVER OF SANITARY LANDFILLS

OpenAIRE

Davorin Kovačić

1994-01-01

The paper deals with the selection of materials for the sea¬ling layer in the final cover of sanitary landfills. The sealing la¬yer is the most critical component of the final cover. Its role is to minimize percolation of water through the final cover. Ma¬terials used for the construction of the sealing layer are either of mineral origin (compacted clay) or geosynthetic (geomem¬brane). They are most often used in combination creating com¬posite liners. Recently alternative materials are also ...

A Model of Clean Water Supply and Improvement of Enviromental Sanitary Conditions in Residential Clusters in The Mekong Delta, Vietnam

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Chi Nguyen Thuy Lan

2015-12-01

Full Text Available In accordance with Decision 99/TTg dated 9/2/1996 and Decision 173/TTg dated 6/11/2001 of the Prime Minister regarding the construction program of residential clusters (residential flood free areas, these residential areas as constructed would be fully equipped with critical infrastructures and services such as water supply and drainage works, toilets with sanitary appropriateness, etc. to ensure environmental sanitary conditions in the residential clusters. However, the actual surveys done in residential clusters in the Mekong Delta show that many arising problems must be addressed to enable the local communities to have better living conditions and ensure the sanitary conditions and environmental safety.

The process of biosorption of heavy metals in bioreactors loaded with sanitary sewage sludge

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A. J. Morais Barros

2006-06-01

Full Text Available This work on the process of biosorption of nickel and chromium in an ascendant continuous-flow, fixed packed-bed bioreactor of sanitary sewage sludge was conducted in a search for solutions to the environmental problem caused by heavy metals. Analysis of the results demonstrated that the absorbent had an extraordinary capacity for biosorption of the heavy metals studied at about 9.0 pH of the effluent, with a removal percentage of over 90.0% for the two metals. Chemometric study results demonstrated that 20 days of the experimental system function were sufficient for achieving the maximum efficiency of sorption of the heavy metals studied by the sanitary sewage sludge employed.

Quantitative susceptibility of Streptococcus suis strains isolated from diseased pigs in seven European countries to antimicrobial agents licenced in veterinary medicine

NARCIS (Netherlands)

Wisselink, H.J.; Veldman, K.T.; Salmon, S.A.; Mevius, D.J.

2006-01-01

The susceptibility of Streptococcus suis strains (n = 384) isolated from diseased pigs in seven European countries to 10 antimicrobial agents was determined. For that purpose a microbroth dilution method was used according to CLSI recommendations. The following antimicrobial agents were tested:

Aberrant chlamydial developmental forms in the gastrointestinal tract of pigs spontaneously and experimentally infected with Chlamydia suis.

Science.gov (United States)

Pospischil, Andreas; Borel, Nicole; Chowdhury, Emdad H; Guscetti, Franco

2009-03-16

The phenomenon of persistence is well known from in vitro studies, where it is associated with the production of aberrant bodies, but its occurrence in vivo is less well documented. The objective of this study was to search for aberrant bodies in intestinal tissues from pigs, describe their ultrastructure, and investigate the suitability of immunohistochemical staining for chlamydial heat shock protein 60 (cHSP60) to detect such forms. Intestinal tissues derived from pigs naturally and experimentally infected with Chlamydia (C.) suis were examined by immunohistochemistry, transmission electron microscopy and immunogold electron microscopy. The chlamydial species involved in the natural infection were determined using an Array Tube Microarray to C. suis and Chlamydophila abortus. Ultrastructurally, aberrant bodies were detected in the gut of both naturally and experimentally infected pigs. Immunogold electron microscopy showed that the aberrant bodies were labeled less strongly than the normal forms by antibodies against LPS and cHSP60 respectively. It was concluded that aberrant bodies occur in vivo in pigs and that the gnotobiotic pig model might be suitable for the study of chlamydial persistence in vivo. The antibody against cHSP60 does not appear to be suitable to specifically detect such forms.

Biogas movements in sanitary landdfills; Movimiento de biogas en rellenos sanitarios

Energy Technology Data Exchange (ETDEWEB)

Vidales A, Humberto

1988-12-31

This paper shows a model to study the physical and kinetic equations that determine the movement and diffusion of the biogas in sanitary landfills. This model for biogas flow was made in function of pressure, temperature, waste porosity and permeability, due to a diffusion coefficient of biogas determination 6 refs., 4 figs.

Prospective technologies and equipment for sanitary hygienic measures for life support systems

Science.gov (United States)

Shumilina, I. V.

Creation of optimal sanitary hygienic conditions is a prerequisite for good health and performance of crews on extended space missions. There is a rich assortment of associated means, methods and equipment developed and experimentally tested in orbital flights. However, over a one-year period a crew of three uses up about 800 kg of ground-supplied wet wipes and towels for personal needs. The degree of closure of life support systems for long-duration orbital flights should be maximized, particularly for interplanetary missions, which exclude any possibility of re-supply. Washing with regenerated water is the ultimate sanitary hygienic goal. That is why it is so important to design devices for crew bathing during long-term space missions. Investigations showed that regeneration of wash water (WW) using membrane processes (reverse osmosis, nanofiltration etc.), unlike sorption, would not require much additional expendables. A two-stage membrane recovery unit eliminated >85% of permeate from real WW with organic and inorganic selectivity of 82 95%. The two-stage WW recovery unit was tested with artificial and real WW containing detergents available for space crews. Investigations into the ways of doing laundry and drying along with which detergents will be the best fit for space flight are also planned. Testing of a technology for water extraction from used textiles using a conventional period of contact of 1 s or more, showed that the humidity of the outgoing air flow neared 100%. Issues related to designing the next generation of space life support systems should consider the benefits of integrating new sanitary hygienic technologies, equipment, and methods.

Sanitary-hygienic and ecological aspects of beryllium production

Energy Technology Data Exchange (ETDEWEB)

Dvinskykh, E.M.; Savchuk, V.V.; Sidorov, V.L.; Slobodin, D.B.; Tuzov, Y.V. [Ulba Metallurgical Plant, Ust-Kamenogorsk (Kazakhstan)

1998-01-01

The Report describes An organization of sanitary-hygienic and ecological control of beryllium production at Ulba metallurgical plant. It involves: (1) the consideration of main methods for protection of beryllium production personnel from unhealthy effect of beryllium, (2) main kinds of filters, used in gas purification systems at different process areas, (3) data on beryllium monitoring in water, soil, on equipment. This Report also outlines problems connected with designing devices for a rapid analysis of beryllium in air as well as problems of beryllium production on ecological situation in the town. (author)

[Pesticide exposure and its repercussion in the health of sanitary agents in the State of Ceará, Brazil].

Science.gov (United States)

Lima, Estelita Pereira; Lopes, Suziy de Matos Bandeira; Amorim, Maria Iracema Mariano de; Araújo, Laura Helena Silva; Neves, Kelly Rose Tavares; Maia, Evanira Rodrigues

2009-01-01

Sanitary agents are the most exposed ones to the effects of insecticides in eradication campaigns since its preparation until its application inside and outside houses. The lack of protection equipment and misinformation on how to use the material properly increase the risk of intoxication. The aim of this work was to analyze the occupational history of vector control sanitary agents in Ceará State, Brazil and its effects on their health. Individual recorded interviews were done with 10 agents who verbally described their life history. The content of these interviews were analyzed through the following categories: agent profile, endemic controls, work conditions, risks and health changes. Health risk situations were described from acute and chronic intoxication to problems with alcoholism. The research showed that sanitary agents have been disrespected as human beings as well as their labor rights, showing negligence by their employers.

Trichurs suis ova theraphy in relapsing multiple sclerosis is safe but without signals of beneficial effect

DEFF Research Database (Denmark)

Voldsgaard, A.; Bager, P.; Garde, E.

2015-01-01

revealed 6 new or enlarged T2 lesions in the run-in period, 7 lesions in the early period and 21 lesions in the late treatment period. Two patients suffered a relapse before treatment and two during treatment. Eight patients developed eosinophilia. The expression of cytokines and transcription factors did...... not change. CONCLUSIONS: In a small group of relapsing multiple sclerosis patients, Trichuris suis oral therapy was well tolerated but without beneficial effect....

Sanitary Landfill Groundwater Monitoring Report, Fourth Quarter 1999 and 1999 Summary

International Nuclear Information System (INIS)

Chase, J.

2000-01-01

A maximum of thirty eight-wells of the LFW series monitor groundwater quality in the Steed Pond Aquifer (Water Table) beneath the Sanitary Landfill Area at the Savannah River Site (SRS). These wells are sampled quarterly to comply with the South Carolina Department of Health and Environmental Control Domestic Water Permit DWP-087A and as part of the SRS Groundwater Monitoring Program. Iron (Total Recoverable), Chloroethene (Vinyl Chloride) and 1,1-Dichloroethane were the most widespread constituents exceeding the Final Primary Drinking Water Standards during 1999. Trichloroethylene, 1,1-Dichloroethylene, 1,2-Dichloroethane, 1,4-Dichlorobenzene, Aluminum (Total Recoverable), Benzene, cis-1,2-Dichloroethylene, Dichlorodifluoromethane, Dichloromethane (Methylene Chloride), Gross Alpha, Mercury (Total Recoverable), Nonvolatile Beta, Tetrachloroethylene, Total Organic Halogens, Trichlorofluoromethane, Tritium also exceeded standards in one or more wells. The groundwater flow direction in the Steed Pond Aquifer (Water Table) beneath the Sanitary Landfill is to the southeast (universal transverse Mercator coordinates). The flow rate in this unit was approximately 144.175 ft/year during first quarter 1999 and 145.27 ft/year during fourth quarter 1999

Cleaner mites: sanitary mutualism in the miniature ecosystem of neotropical bee nests.

Science.gov (United States)

Biani, Natalia B; Mueller, Ulrich G; Wcislo, William T

2009-06-01

Cleaning symbioses represent classic models of mutualism, and some bee mites are thought to perform cleaning services for their hosts in exchange for suitable environments for reproduction and dispersal. These mutual benefits, however, have not been rigorously demonstrated. We tested the sanitary role of bee mites by correlating mite loads with fungal contamination in natural nests of Megalopta genalis and Megalopta ecuadoria and by experimentally manipulating mite loads in artificial cells with developing brood. Field observations revealed significant correlations between the presence of mites and the absence of fungi inside the brood cells, as well as between the absence of mites and increased bee mortality. Likewise, experimental brood cells with mites have fewer fungal colonies than do cells without mites. Field observations and experimental manipulations, therefore, provide clear evidence of the sanitary effect of mites in nests of Megalopta bees. This bee-mite association constitutes one of the few examples of terrestrial cleaning mutualisms.

Irregularidades sanitárias como marcador de risco à saúde: um desafio para a vigilância sanitária / Sanitary Disparities as risks markers health: a challenge to sanitary surveillance

Directory of Open Access Journals (Sweden)

Fernanda Pini Freitas

2013-02-01

Full Text Available O estudo, de caráter exploratório, do tipo levantamento retrospectivo, objetivou identifi car as irregularidades sanitárias nos estabelecimentos e equipamentos de assistência de alta complexidade e de interesse à saúde do município de Franca-SP, registradas no Sistema de Informação em Vigilância Sanitária, no período de agosto de 2008 a julho de 2009, como marcador de risco à saúde. Do total de 186 serviços, 59 (31,72% apresentaram irregularidades sanitárias, categorizadas em nove eixos: documentação, estrutura física, recursos humanos, qualidade de produtos, manutenção preventiva de equipamentos, processo de esterilização, resíduos de saúde, higienização do ambiente e equipamento de proteção individual; e 164 (88,17% apresentaram condições de baixo risco, 21 (11,29% de médio e um serviço apresentou risco alto. Os resultados demonstram que as irregularidades sanitárias podem comprometer a qualidade do serviço ou do produto oferecido e gerar riscos à saúde dos usuários, consumidores e trabalhadores. ---------------------------------------------------------------------------------------- This study, as an exploratory one, retrospective survey-type, aimed to identify the sanitary disparities in the high complexity facilities and assistance tools and concerned to Franca – SP heath, registered in the Sanitary Surveillance Information System, in August 2008 to July 2009 as risks markers to health. From the amount of 186 services, 58 (31,72% presented sanitary disparities, categorized into nine axes: documentation, physical frames, human resources, products qualities, tool’s preventive maintenance, sterilization process. Health waste, environment hygiene and individual protection equipments, 164 (88, 17% presented a law risk condition, 21 (11,29% are average, and only one presented a high risk level. Results shows that such sanitary disparities may comprise the service or the offered product quality and

Proposal for the reinforcement of the environmental sanitary safety; Proposition pour un renforcement de la securite sanitaire environnementale

Energy Technology Data Exchange (ETDEWEB)

Grzegrzulka, O; Aschieri, A [Assemblee Nationale, 75 - Paris (France)

1998-11-01

Facing the context of the public reliance crisis, the sanitary risks bound to the environment became more and more important. This report asked by the first Minister of France, takes stock on the government policy in this domain and the today institutions. The first part defines the problematic of the sanitary risks bound to the environment, the second one shows the non adapted answer offered by the actual government and the last one proposes new procedures and actions.

Protection of pigs against challenge with virulent Streptococcus suis serotype 2 strains by a muramidase-released protein and extracellular factor vaccine

NARCIS (Netherlands)

Wisselink, H.J.; Vecht, U.; Stockhofe Zurwieden, N.; Smith, H.E.

2001-01-01

The efficacy of a muramidase-released protein (MRP) and extracellular factor (EF) vaccine in preventing infection and disease in pigs challenged either with a homologous or a heterologous Streptococcus suis serotype 2 strain (MRP EF ) was compared with the efficacy of a vaccine containing

Eco-efficient concretes: the effects of using recycled ceramic material from sanitary installations on the mechanical properties of concrete.

Science.gov (United States)

Guerra, I; Vivar, I; Llamas, B; Juan, A; Moran, J

2009-02-01

The aim of this research was to investigate some of the physical and mechanical properties of concrete mixed under laboratory conditions, where different proportions of coarse aggregate materials were substituted by porcelain from sanitary installations. The results of the tests show that the concrete produced has the same mechanical characteristics as conventional concrete, thus opening a door to selective recycling of sanitary porcelain and its use in the production of concrete.

Evolution of sanitary-epidemiological services in Poland in the years 1944-2014

Directory of Open Access Journals (Sweden)

Marek L. Grabowski

2017-11-01

The pre-accession preparation to the European Union (EU strongly accelerated the development of sanitary-epidemiological services in Poland. Polish accession to the European Union has promoted the implementation of the WHO document “Health for All in the 21st Century” and the reduction of “health inequalities”.

Attitudes towards the Use of the Medical and Sanitary Services in Iran

Science.gov (United States)

Mohseni, Manouchehr

1977-01-01

Describes research methodology and findings of a survey in Iran to determine relationships between educational level, age, residence, and other variables involved in attitudes regarding the use of medical and sanitary services. Public health education is seen to be needed. For journal availability, see SO 506 019. (Author/AV)

30 CFR 71.400 - Bathing facilities; change rooms; sanitary flush toilet facilities.

Science.gov (United States)

2010-07-01

... flush toilet facilities. 71.400 Section 71.400 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY HEALTH STANDARDS-SURFACE COAL MINES AND SURFACE... installations and at the surface worksites of such mine. (Note: Sanitary facilities at surface work areas of...

Sanitary and Phytosanitary Measures: The Case of Mexican Avocados

OpenAIRE

Bakshi, Nishita

2003-01-01

This thesis examines the effects on demand, supply, imports, and prices of partial easing of sanitary and phytosanitary (SPS) barriers to trade in the U.S. market in the case of Mexican avocados. The SPS Agreement plays a role in the avocado market studied here through its implications for negotiations between countries that have not utilized the formal channels of the WTO for resolving disputes. A quarantine in place from 1914 until very recently banned entry of Mexican avocados into the...

Water radiological sanitary control of Veracruz State

International Nuclear Information System (INIS)

Carreon G, E.; Vazquez C, J. A.; Aguilar P, M. del C.; Parissi C, A.

2014-10-01

This work is carried out in Veracruz State covering over 11 jurisdictions of the State (Panuco, Tuxpan, Poza Rica, Martinez de la Torre, Xalapa, Cordoba, Orizaba, Veracruz, Cosamaloapan, San Andres Tuxtla and Coatzacoalcos). The sampling was realized in a period from 2009 to 2013 analyzing home drinking water, supply sources and wells, the sampling was done by the sanitary checkers of different jurisdictions with approved methods and the methodology was validated at the State Laboratory of Public Health. 1637 samples were analyzed by counting equipment Tennelec Canberra series 5 and a gas supply system P-10 with calibration curves for alpha and gross beta. The results of measurements ranging from 0.07 to 0.25 Bq/L in the activity concentration gross alpha annual average, an gross beta were from 0.12 to 0.17 Bq/L in the activity concentration gross beta annual average, and with a concentration range of alpha activity up to 0.62 and a minimum 0.02, and the concentration of beta activity of a maximum value 1.54 and a minimum 0.02, taking also as resulted in five years of analysis only 1.16% of the analyzed samples (19 samples) showed a value of alpha activity concentration above the minimum detectable concentration and 62.43% (1022 samples) of the analyzed samples showed a value of beta activity concentration above the minimum detectable concentration, is also clear that the results of the sanitary jurisdictions of Panuco and Tuxpan not have corresponding activity values for the years 2009, 2011-2013 except 2010. We can conclude that the regular measurements of alpha and gross beta activity in water are invaluable for timely detection of radioactive contamination. (Author)

Sanitary effect of gamma irradiation on sewage sludge

International Nuclear Information System (INIS)

Hess, E.; Breer, C.

1975-01-01

Our investigations prove that sludge contains Salmonellae in more than 90% of samples. The maximum number of organisms was 10 7 per litre. One of our most important findings was the fact that neither aerobic stabilization nor anaerobic digestion significantly reduces contamination with Salmonellae. Moreover we found that Salmonellae in sewage sludge spread on grass may survive up to 72 weeks. Fertilizing with unsanitized sludge may therefore lead to transmission from plant to animal. The increasing number of Salmonella carriers among our herds of cattle and their striking accumulation during the grazing period demonstrate that such transmission represents a growing danger. Sanitation of sludge to be used as fertilizer is therefore urgent. In our investigation of the sanitary effect of pasteurization (70degC for 30 min) and of gamma irradiation on sewage sludge, we examined the number of Enterobacteriaceae before and after irradiation in 259 specimens of sludge from 44 different sewage disposal plants. The doses applied were 100, 200, 300, 400 and also 500 krad. We found a linear reduction of Enterobacteriaceae with increasing doses; a dose of 300 krad resulted in a death rate of 10 4 - 10 8 , occasionally 10 9 Enterobacteriaceae; and there were less than 10 Enterobacteriaceae per gram in 97.2% of the samples irradiated with 300 krad. The results of these model experiments could be completely confirmed under practical conditions in the irradiation plant of Geiselbullach. The sanitary effect of gamma irradiation with 300-350 krad, determined by the reduction in Enterobacteriaceae, was equivalent to the effect of heat treatment by pasteurization. (author)

LA COMUNICAZIONE POLITICA SUI SOCIAL NETWORK: UN’ANALISI LINGUISTICA

Directory of Open Access Journals (Sweden)

Daniele Spoladore

2014-07-01

Full Text Available Questo lavoro si propone di analizzare le scelte linguistiche dei soggetti politici che utilizzano Facebook e Twitter per rivolgersi al sempre più frammentato panorama dei potenziali elettori. In particolare, analizza le tendenze linguistiche comuni che questi social network hanno fatto emergere nel rapporto tra politica, web e lettori. Cominciando dall’analisi della presenza di parlamentari e senatori su Facebook e Twitter, si cerca di riassumere le principali caratteristiche delle due piattaforme, per giungere ad una classificazione dei testi prodotti attraverso di esse; si analizza la scrittura di post e tweet, sottolineando la presenza di espedienti tipici del mezzo e valutando il loro effetto sui lettori, e si studiano le scelte sintattiche e morfosintattiche in relazione alla struttura e alle possibilità dei due social network. In ultima analisi si osservano le scelte lessicali dei soggetti politici, studiandone i campi semantici e la quantità di tecnicismi. Infine, si cerca di compiere una valutazione delle due differenti tipologie di trasmesso scritto rinvenute nei campioni, osservando come ognuna di esse abbia uno scopo ben preciso nell’economia della comunicazione politica sui social network. Political communication policy in social networks: a language analysis  Daniele Spoladore This paper aims to analyze the linguistic choices of politicians who use Facebook and Twitter to address the increasingly fragmented landscape of potential voters. In particular, it analyzes the common language trends that these social networks have revealed in the relationship between politics, the web and readers. Starting from the analysis of the presence of MPs and senators on Facebook and Twitter, we try to summarize the key features of the two platforms, in order to arrive at a classification of the texts produced using them.  We analyzed posts and tweets, emphasizing the  typical characteristics of these means and evaluating their effect on

The first report on Cryptosporidium suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa) (Czech Republic)

Czech Academy of Sciences Publication Activity Database

Němejc, K.; Sak, Bohumil; Květoňová, Dana; Hanzal, V.; Jeníková, Martina; Kváč, Martin

2012-01-01

Roč. 184, 2/4 (2012), 122-125 ISSN 0304-4017 Grant - others:Mšk(CZ) 6007665806 Institutional research plan: CEZ:AV0Z60220518; CEZ:AV0Z50450515 Keywords : Cryptosporidium suis * Cryptosporidium pig genotype II * Eurasian wild boar * SSU * PCR Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.381, year: 2012

MATERIALS FOR THE FINAL COVER OF SANITARY LANDFILLS

Directory of Open Access Journals (Sweden)

Davorin Kovačić

1994-12-01

Full Text Available The paper deals with the selection of materials for the sea¬ling layer in the final cover of sanitary landfills. The sealing la¬yer is the most critical component of the final cover. Its role is to minimize percolation of water through the final cover. Ma¬terials used for the construction of the sealing layer are either of mineral origin (compacted clay or geosynthetic (geomem¬brane. They are most often used in combination creating com¬posite liners. Recently alternative materials are also used like paper mill sludge or discarded swelling clay.

Banana fertigation with treated sanitary wastewater: postharvest and microbiological quality

Directory of Open Access Journals (Sweden)

Pablo Fernando Santos Alves

2017-06-01

Full Text Available Sewage may serve as a source of water and nutrients for plants. In this study, the effects of fertigation with treated sanitary wastewater from Janaúba Sewage Treatment Plant were evaluated on the postharvest and microbiological quality of ‘Prata-Anã’ banana. A randomized block experimental design was used. Four concentrations of wastewater were tested (70, 130, 170, and 200% of 150 kg ha-1 sodium. A wastewater-free control treatment was used for comparison. Two crop cycles were assessed for postharvest and microbiological quality. The parameters measured included total soluble solids, titratable acidity, total soluble solids/titratable acidity ratio, pH, total coliforms, and fecal coliforms on both the peel and the pulp. In the first crop cycle, both soluble solids and fruit pulp pH decreased as wastewater level increased up to a maximum of 141.5%. These correlations were not observed in the second cycle. Wastewater management did not affect the titratable acidity of the soluble solids. The agricultural application of treated sanitary wastewater provided banana fruits with a microbiological profile similar to that obtained with the control (pure water and with mineral fertilizers. A microbial balance is necessary to maintain the nutritional status of the banana crop.

Mineralogy and microstructure of hydrated phases during the pozzolanic reaction in the sanitary ware waste/Ca(OH)2 system

OpenAIRE

Medina, César; Sáez del Bosque, Isabel F.; Asensio, Eloy; Frías, Moisés; Sánchez de Rojas, M Isabel

2016-01-01

Despite technological improvements in its production process, the sanitary ware industry inevitably generates a certain volume of discards, products whose quality is not up to standard. The present paper is the first to scientifically explore claybased sanitary ware waste (SW) with a view to its valorization as an addition in the design of new, more environmentally friendly cements. The focus is on characterization of the waste and its pozzolanicity, as well as the struct...

The removal of ammonia from sanitary landfill leachate using a series of shallow waste stabilization ponds.

Science.gov (United States)

Leite, V D; Pearson, H W; de Sousa, J T; Lopes, W S; de Luna, M L D

2011-01-01

This study evaluated the efficiency of a shallow (0.5 m deep) waste stabilization pond series to remove high concentrations of ammonia from sanitary landfill leachate. The pond system was located at EXTRABES, Campina Grande, Paraiba, Northeast Brazil. The pond series was fed with sanitary landfill leachate transported by road tanker to the experimental site from the sanitary landfill of the City of Joao Pessoa, Paraiba. The ammoniacal-N surface loading on the first pond of the series was equivalent to 364 kg ha(-1) d(-1) and the COD surface loading equivalent to 3,690 kg ha(-1) d(-1). The maximum mean ammonia removal efficiency was 99.5% achieved by the third pond in the series which had an effluent concentration of 5.3 mg L(-1) ammoniacal-N for an accumulative HRT of 39.5 days. The removal process was mainly attributed to ammonia volatilization (stripping) from the pond surfaces as a result of high surface pH values and water temperatures of 22-26°C. Shallow pond systems would appear to be a promising technology for stripping ammonia from landfill leachate under tropical conditions.

Hygienic assessment of habitat adverse social and sanitary factors in the Altai Krai

Directory of Open Access Journals (Sweden)

A.A. Ushakov

2015-12-01

Full Text Available The materials on health status, socio-economic, sanitary living conditions of the population of the Altai Krai have been analyzed. The comparative analysis of the conditions of life and health outcomes in urban and rural settlements’ population has been performed. The estimation of health indicators’ correlation has been carried out in children of age group 0-1 years, children of age group 0-14 years, teens of age group 15-17 years and adults of age group over 18 years, depending on the type (urban or rural of settlement with indicators of social and sanitary environmental factors. Regression equations for the health outcomes of different age groups on the level of hygiene and social environmental factors are set, regional critical (reference values are justified. The hygienic assessment of unfavorable social, health and sanitation of the environment on the health of the population in the Altai Krai is provided.

The Use of Heat-Resistant Concrete Made with Ceramic Sanitary Ware Waste for a Thermal Energy Storage

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Paweł Ogrodnik

2017-12-01

Full Text Available The paper presents the results obtained in the course of a study on the concrete made of aggregate obtained from wastes of sanitary ceramics. Previous examinations proved high in strength and durability of concrete of this type, and it showed a resistance to high temperatures. The material was classified as a fireproof concrete. While searching for the optimal applications of such concrete, a series of examinations and analyses on its thermal energy storage (TES properties were performed. This paper describes the two-stage experiment on the thermal behavior of the concrete made with sanitary ceramic wastes during cooling processes in comparison to different building materials subjected to the same thermal conditions. On the basis of the thermal, infrared analysis, and suitable calculations, the thermal power and the ability of the composite to store thermal energy was estimated. Finally, it was stated that the concrete made of sanitary ceramic waste aggregate and alumina cement can be recommended as a heat-accumulating material, and in combination with high durability can be used, e.g., for the construction of fireplace bodies.

An Analysis of the Sanitary and Welfare Conditions of Tourism Centers in Lorestan Province: A Qualitative Study

Directory of Open Access Journals (Sweden)

Koroush Saki

2018-03-01

Full Text Available Background and Objectives: Due to the rapid growth of tourism industry, appropriate management and supervision of tourism centers by improving the sanitary, welfare and security situation of these centers is essential. Accordingly, this study aimed to investigate sanitary and welfare status of tourism centers in Lorestan province. Material and Methods: This research is a qualitative study conducted through semi-structured Interview with tourists. Data analysis was done using content analysis. After collecting and analyzing data, findings were classified into themes and sub-themes. Results: The findings were categorized into 6 main themes and 24 sub-themes. The main themes were general sanitary conditions, welfare facilities, transportation, healthcare services, security, and management and supervision. Conclusion: According to the findings and participants’ points of view, tourists are not satisfied with health care status of tourist centers. Therefore, in order to minimize the health problems of tourism centers and maximize attracting tourists, short-term and long-term plans should be put on the agenda of tourism managers and stakeholders of the Lorestan province.

Role of Community in Swachh Bharat Mission. Their Knowledge, Attitude and Practices of Sanitary Latrine Usage in Rural Areas, Tamil Nadu.

Science.gov (United States)

Anuradha, R; Dutta, Ruma; Raja, J Dinesh; Lawrence, D; Timsi, J; Sivaprakasam, P

2017-01-01

In most developing countries, open defecation is the 'way of life'. This practice is considered as the most serious health and environmental hazard. Prime Minister of India launched the "Swachh Bharat Mission" to accelerate the efforts for achieving universal sanitation coverage and to put focus on sanitation. To find the knowledge, attitude and practices of sanitary latrines usage in rural area, Tamil Nadu. This was a cross sectional study conducted among rural population in Kuthambakkam village, Tamil Nadu. There were a total of 1175 households in Kuthambakkam village. These households were serially numbered and of these a sample of 275 households were selected for the study using simple random sampling technique by lottery method. A structured questionnaire was used to collect information regarding the background characteristics, their knowledge, attitude and practices towards sanitary latrines usage. Descriptive statistics were calculated for background variables, the prevalence of sanitary latrines usage and open air defecation. Association between factors responsible for open air defecation was found by using chi square test. The prevalence of usage of household sanitary latrine and community latrines was 62.5% and 4.3% respectively. The prevalence of open air defecation among the study participants was 33.1%.Significant association was found between low standard of living and open air defecation practice. To solve the problem of underutilization of sanitary latrines, planning and conducting Information Education Communication activities is very essential. Effective political and administrative support is needed to scale up the sanitation program.

Final Disposal of Solid Waste in Sanitary Landfills and Human Health

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Gustavo Silveira Graudenz

2012-06-01

Full Text Available This article presents a critical review of scientific literature on waste sanitary landfills and its effects on human health, with an approach to the adverse effects that are most commonly associated to living near waste landfills. The health variables included were low birth weight, congenital abnormalities, some types of neoplasms, allergies, asthma and other respiratory diseases using the MEDLINE, LILACS and CAPES’ thesis post graduation database for systematic review. In spite of the fact that some studies indicate positive asssociation between health risks and living close to landfills, the majority of the studies, mainly the most recent ones, do not demonstrate a significant health risk in this condition. Some common limitations and bias of the work in the field are discussed. The lack of direct quantification of exposure, lack of prospective approach and no comparaison of the different types and quality of management of the residues are common limitations to most studies. So far, there is weak evidence to support significant epidemiological health risks associated to landfills. More interdisciplinary research should improve the knoledge of the health risks related to living in the proximity to sanitary landfills.

PHYSIOLOGICAL AND SANITARY QUALITY OF DESICCATED AND STORED AZUKI BEAN SEEDS

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CÁSSIO JARDIM TAVARES

2016-01-01

Full Text Available The objective of this research was to evaluate the effects of using different herbicides as desiccants in pre - harvest and the effects of storage on the physiological and sanitary quality of azuki bean seeds ( Vigna angularis Willd. The experiment was arranged in a randomized complete block design in a split plot scheme, with four replications. Four herbicides were tested: paraquat (400 g a.i. ha - 1 , glufosinate ammonium (400 g a.i. ha - 1 , glyphosate (720 g a.i. ha - 1 , flumioxazin (30 g a.i. ha - 1 and a control without herbicide application. In the subplots seed quality was tested in two evaluation periods: at harvest and six months after harvest. Desiccant was applied when the azuki beans were physiologically mature. We assessed the physiological and sanitary quality of the seeds using a vigour and seed health test. The use of glyphosate resulted in a higher incidence of abnormal seedlings and reduced size and weight of the seedlings. With paraquat and flumioxazin the physiological quality was maintained and there was reduced pathogen infestation in the seeds six months after harvest. Storage affected the physiological quality of the azuki bean seeds.

30 CFR 71.500 - Sanitary toilet facilities at surface work sites; installation requirements.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Sanitary toilet facilities at surface work sites; installation requirements. 71.500 Section 71.500 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY HEALTH STANDARDS-SURFACE COAL MINES AND...

Evaluation of new location of Isfahan′s sanitary landfill site with Oleckno method

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Maryam Salimi

2013-01-01

Full Text Available Aims: The objective of present study was to evaluate the new location of Isfahan solid waste sanitary landfill using Geographical Information System (GIS based on the Oleckno index method (OIM. Materials and Methods: This study was on the field- and library-based data collection and surveys of relevant data. Assessment parameters included average annual rainfall, soil type and ground water beneath and adjucent to the landfill site. To analyze data, ArcGIS version 9.3 was used. Results: In 2010 the total rainfall in the landfill location was less than 150 mm/year. The soil type was clay loam, and the average distance from the floor of the landfill to the groundwater level was 3-9 meters. As calculated results showed that, the Oleckno index (OI score in the study area was 40. Conclusion: The new Isfahan′s sanitary solid waste landfill site had a good OI and the possibility of contamination of groundwater by leachate production based on this method also was low.

Mathematical numeric models for assessing the groundwater pollution from Sanitary landfills

Science.gov (United States)

Petrov, Vasil; Stoyanov, Nikolay; Sotinev, Petar

2014-05-01

Landfills are among the most common sources of pollution in ground water. Their widespread deployment, prolonged usage and the serious damage they cause to all of the elements of the environment are the reasons, which make the study of the problem particularly relevant. Most dangerous of all are the open dumps used until the middle of the twentieth century, from which large amounts of liquid emissions flowed freely (landfill infiltrate). In recent decades, the problem is solved by the construction of sanitary landfills in which they bury waste or solid residue from waste utilization plants. The bottom and the sides of the sanitary landfills are covered with a protective waterproof screen made of clay and polyethylene and the landfill infiltrate is led outside through a drainage system. This method of disposal severely limits any leakage of gas and liquid emissions into the environment and virtually eliminates the possibility of contamination. The main topic in the conducted hydrogeological study was a quantitative assessment of groundwater pollution and the environmental effects of re-landfilling of an old open dump into a new sanitary landfill, following the example of the municipal landfill of Asenovgrad, Bulgaria. The study includes: 1.A set of drilling, geophysical and hydrogeological field and laboratory studies on: -the definition and designation of the spatial limits of the main hydrogeological units; -identification of filtration parameters and migration characteristics of the main hydrogeological units; -clarifying the conditions for the sustentation and drainage of groundwater; -determininng the structure of the filtration field; -identifying and assessing the size and the extent of groundwater contamination from the old open dump . 2.Mathematical numeric models of migration and entry conditions of contaminants below the bottom of the landfill unit, with which the natural protection of the geological environment, the protective effect of the engineering

Role of community in swachh bharat mission. their knowledge, attitude and practices of sanitary latrine usage in rural areas, Tamil Nadu

Directory of Open Access Journals (Sweden)

R Anuradha

2017-01-01

Full Text Available Background: In most developing countries, open defecation is the 'way of life'. This practice is considered as the most serious health and environmental hazard. Prime Minister of India launched the “Swachh Bharat Mission” to accelerate the efforts for achieving universal sanitation coverage and to put focus on sanitation. Objective: To find the knowledge, attitude and practices of sanitary latrines usage in rural area, Tamil Nadu. Materials and Methods: This was a cross sectional study conducted among rural population in Kuthambakkam village, Tamil Nadu. There were a total of 1175 households in Kuthambakkam village. These households were serially numbered and of these a sample of 275 households were selected for the study using simple random sampling technique by lottery method. A structured questionnaire was used to collect information regarding the background characteristics, their knowledge, attitude and practices towards sanitary latrines usage. Descriptive statistics were calculated for background variables, the prevalence of sanitary latrines usage and open air defecation. Association between factors responsible for open air defecation was found by using chi square test. Results: The prevalence of usage of household sanitary latrine and community latrines was 62.5% and 4.3% respectively. The prevalence of open air defecation among the study participants was 33.1%.Significant association was found between low standard of living and open air defecation practice. Conclusions: To solve the problem of underutilization of sanitary latrines, planning and conducting Information Education Communication activities is very essential. Effective political and administrative support is needed to scale up the sanitation program.

A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates

DEFF Research Database (Denmark)

Chen, Chen; Tang, Jiaqi; Dong, Wei

2007-01-01

shock syndrome (STSS), which was originally associated with Streptococcus pyogenes (GAS) in Streptococci. However, the molecular mechanisms underlying STSS are poorly understood. METHODS AND FINDINGS: To elucidate the genetic determinants of STSS caused by SS2, whole genome sequencing of 3 different......BACKGROUND: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as an etiological agent for streptococcal toxic...

A review of the Y-12 Plant discharge of enriched uranium to the sanitary sewer (DEUSS)

International Nuclear Information System (INIS)

1991-09-01

The Oak Ridge Y-12 Plant is situated adjacent to the Oak Ridge city limits and is operated by the United States Department of Energy (DOE). The Y-12 Plant is located on 4,860 acres, which is collectively referred to as the Y-12 Plant site. Among the missions for which the facility is in existence are producing nuclear weapons components, supporting weapon design laboratories, and processing special nuclear materials (SNM). The Y-12 Plant is under the regulatory guidance of DOE Order 5400.5 and has complied with the technical requirements governing SNM since its issue. However, an in-depth review with appropriate documentation had not been performed, prior to the effect presented herein, to substantiate this claim. As a result of the solid waste issue, it was determined that other types of waste should be formally reviewed for content with respect to SNM. Therefore, a project was formed to investigate the conveyance of SNM through the sanitary sewer system. It is emphasized that this project addresses only effluent from the sanitary sewer system and not the storm sewer system. The project reviewed sanitary sewer data both for the Y-12 Plant and the Y-12 Plant site

A review of the Y-12 Plant discharge of enriched uranium to the sanitary sewer (DEUSS)

Energy Technology Data Exchange (ETDEWEB)

1991-09-01

The Oak Ridge Y-12 Plant is situated adjacent to the Oak Ridge city limits and is operated by the United States Department of Energy (DOE). The Y-12 Plant is located on 4,860 acres, which is collectively referred to as the Y-12 Plant site. Among the missions for which the facility is in existence are producing nuclear weapons components, supporting weapon design laboratories, and processing special nuclear materials (SNM). The Y-12 Plant is under the regulatory guidance of DOE Order 5400.5 and has complied with the technical requirements governing SNM since its issue. However, an in-depth review with appropriate documentation had not been performed, prior to the effect presented herein, to substantiate this claim. As a result of the solid waste issue, it was determined that other types of waste should be formally reviewed for content with respect to SNM. Therefore, a project was formed to investigate the conveyance of SNM through the sanitary sewer system. It is emphasized that this project addresses only effluent from the sanitary sewer system and not the storm sewer system. The project reviewed sanitary sewer data both for the Y-12 Plant and the Y-12 Plant site.

Comparative Genome Analyses of Streptococcus suis Isolates from Endocarditis Demonstrate Persistence of Dual Phenotypic Clones.

Science.gov (United States)

Tohya, Mari; Watanabe, Takayasu; Maruyama, Fumito; Arai, Sakura; Ota, Atsushi; Athey, Taryn B T; Fittipaldi, Nahuel; Nakagawa, Ichiro; Sekizaki, Tsutomu

2016-01-01

Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S. suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs), ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels) ranging from one to 104 bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated) bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes.

Comparative Genome Analyses of Streptococcus suis Isolates from Endocarditis Demonstrate Persistence of Dual Phenotypic Clones.

Directory of Open Access Journals (Sweden)

Mari Tohya

Full Text Available Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S. suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs, ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels ranging from one to 104 bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes.

Comparative Genome Analyses of Streptococcus suis Isolates from Endocarditis Demonstrate Persistence of Dual Phenotypic Clones

Science.gov (United States)

Tohya, Mari; Watanabe, Takayasu; Maruyama, Fumito; Arai, Sakura; Ota, Atsushi; Athey, Taryn B. T.; Fittipaldi, Nahuel; Nakagawa, Ichiro; Sekizaki, Tsutomu

2016-01-01

Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S. suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs), ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels) ranging from one to 104 bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated) bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes. PMID:27433935

Health care for older people in Italy: The U.L.I.S.S.E. Project (Un link informatico sui servizi sanitari esistenti per l'anziano - a computerized network on health care services for older people).

Science.gov (United States)

Lattanzio, F; Mussi, C; Scafato, E; Ruggiero, C; Dell'Aquila, G; Pedone, C; Mammarella, F; Galluzzo, L; Salvioli, G; Senin, U; Carbonin, P U; Bernabei, R; Cherubini, A

2010-03-01

The U.L.I.S.S.E. study is aimed at describing older patients who are cared for in hospitals, home care or nursing homes in Italy. The U.L.I.S.S.E. study is an observational multicenter prospective 1-year study. Overall, 23 acute geriatric or internal medicine hospital units, 11 home care services and 31 nursing homes participated in the study. The patient's evaluation was performed using comprehensive geriatric assessment instruments, i.e. the interRAI Minimum Data Set, while data on service characteristics were recorded using ad-hoc designed questionnaires. The older subjects who are in need of acute and long term care in Italy have similar characteristics: their mean age is higher than 80 years, they have a high level of disability in ADL, an important multimorbidity, and are treated with several drugs. The prevalence of cognitive impairment is particularly high in nursing homes, where almost 70% of residents suffer from it and 40% have severe cognitive impairment. On the other hand, there is a shortage of health care services, which are heterogeneous and fragmented. Health care services for older people in Italy are currently inadequate to manage the complexity of the older patients. An important effort should be undertaken to create a more integrated health care system.

Innovative in situ treatment approach for DOE Savannah River Site Sanitary Landfill

International Nuclear Information System (INIS)

Knapp, J.; Suer, A.

1994-01-01

Pursuant to a settlement agreement reached between the US Department of Energy (DOE) and the South Carolina Department of Health and Environmental Control (SCDHEC), the Sanitary Landfill at the Savannah River Site will be closed. This paper addresses the approach used to select the innovative in situ treatment alternative for the groundwater and the vadose zone associated with the landfill

Efficacy of sulfonamides and Baycox(®) against Isospora suis in experimental infections of suckling piglets.

Science.gov (United States)

Joachim, Anja; Mundt, Hans-Christian

2011-12-01

Sulfonamide treatment of piglets against neonatal coccidiosis has frequently been suggested in the literature. In order to evaluate the efficacy of sulfonamides against experimental Isospora suis infections in suckling piglets (oral infection with 1,500 sporulated oocysts of I. suis per piglet on the fourth day of life), two trials were conducted. In trial I, oral sulfadimidine (group Sulfa-Oral) was applied in doses of 100 mg/kg of body weight (BW) 1 day before infection and 75 mg/kg BW daily for the following 5 days, and sulfamethoxypyrimidine (SMP) was applied parenterally in daily doses of 75 mg/kg BW for the same time period. In trial II, SMP was applied parenterally in doses of 75 mg/kg BW (a) from the day of infection daily for 7 days (SMP-Standard), (b) for 2 days starting on the day of infection (SMP-Early), (c) for 3 days starting 2 days post-infection (d.p.i.; SMP-Middle), (d) for 2 days starting 5 d.p.i. (SMP-Late), and (e) every other day from the day of infection until 6 d.p.i. (SMP-Alternating), as well as (f) orally in doses of 75 mg/kg BW from the day of infection for 7 days (SMP-Oral). The sulfonamide-treated groups were compared to a toltrazuril-treated group (single oral treatment with Baycox® 5% suspension, 20 mg/kg BW 2 d.p.i.) and to a water-treated Control group. Each group consisted of seven to nine piglets. The parameters evaluated were oocyst excretion and fecal consistency/diarrhea from 4 to 15 d.p.i. Sulfa-Oral, SMP-Early, and SMP-Late had no significant effect in reduction of oocyst excretion and diarrhea, whereas treatment for 3-7 days with SMP reduced both parasite shedding and diarrhea significantly. Oral treatment with SMP was comparable to parenteral application. Baycox® in a single application had the most pronounced effect and completely suppressed oocyst excretion and diarrhea during the examination period. It could be shown that repeated application of sulfonamides, provided that the appropriate time period after infection

Analysis of glycerin waste in A-Area sanitary treatment facility material

International Nuclear Information System (INIS)

1995-01-01

TNX has a large supply of 55 gallon drums containing pure glycerin and glycerin with additives. The glycerin drums were procured to simulate the glass stream in a pilot-scale melter process at TNX. Since the glycerin was not used for this process, TNX is looking at disposing the material in a sanitary waste treatment facility onsite. The effect of adding the contents of the drums to sewage bacteria was tested. A drum of pure glycerin and a drum of glycerin mixed with lithium chloride were tested. The test consisted of mixing sanitary sludge material with the glycerin material. The purpose of the test was to determine if the glycerin impacted the aerobic bacterial population. The bacterial densities were determined by taking samples from the sludge/glycerin mixtures and using aerobic plate count methods. The total organic carbon (TOC) levels were measured before and after testing. The results indicate that the cell density of the aerobic bacteria increased with the addition of glycerin and the glycerin mixture and the TOC removal rate was different for all tests. Disposal of glycerin in the wastewater treatment facilities should pose no problems. Additional testing and analysis of the mixed samples should be done before its disposal in a waste water treatment facility

Parasite-specific IL-4 responses in Ascaris suum and Trichuris suis-infected pigs evaluated by ELISPOT

DEFF Research Database (Denmark)

Steenhard, N. R.; Kringel, H.; Roepstorff, A.

2007-01-01

method is a valuable tool for future experimental settings as it enables repeated and parasite-specific measurement of IL-4 at protein level when investigating, for example, immunomodulatory properties of helminths. Furthermore, the method could be used to identify specific parasite antigens inducing IL......The objective of the present study was to develop an ELISPOT method to measure parasite-specific IL-4 producing cells during experimental Ascaris suum and Trichuris suis infections in pigs. In many experimental settings it is useful to be able to measure changes in specifically induced cytokines...

Biogas recuperation in sanitary landfills; Recuperacao de biogas em aterros sanitarios

Energy Technology Data Exchange (ETDEWEB)

Lima, Luiz Mario Queiroz [Companhia Paulista de Forca e Luz (CPFL), Campinas, SP (Brazil)

1988-12-31

This work shoes a retrospective about recovering biogas activity in sanitary landfill, where in the genesis of methane is emphasized. Also, a conceptual review was made and discussed focusing the technological aspects of the landfill methods. Further, two important aspects of the genesis of methane were discussed: the enhancement mechanisms for acceleration of the formation of methane and the production of biological input from leachate in landfill. (author) 36 refs., 3 figs.

An effective and comprehensive model for optimal rehabilitation of separate sanitary sewer systems.

Science.gov (United States)

Diogo, António Freire; Barros, Luís Tiago; Santos, Joana; Temido, Jorge Santos

2018-01-15

In the field of rehabilitation of separate sanitary sewer systems, a large number of technical, environmental, and economic aspects are often relevant in the decision-making process, which may be modelled as a multi-objective optimization problem. Examples are those related with the operation and assessment of networks, optimization of structural, hydraulic, sanitary, and environmental performance, rehabilitation programmes, and execution works. In particular, the cost of investment, operation and maintenance needed to reduce or eliminate Infiltration from the underground water table and Inflows of storm water surface runoff (I/I) using rehabilitation techniques or related methods can be significantly lower than the cost of transporting and treating these flows throughout the lifespan of the systems or period studied. This paper presents a comprehensive I/I cost-benefit approach for rehabilitation that explicitly considers all elements of the systems and shows how the approximation is incorporated as an objective function in a general evolutionary multi-objective optimization model. It takes into account network performance and wastewater treatment costs, average values of several input variables, and rates that can reflect the adoption of different predictable or limiting scenarios. The approach can be used as a practical and fast tool to support decision-making in sewer network rehabilitation in any phase of a project. The fundamental aspects, modelling, implementation details and preliminary results of a two-objective optimization rehabilitation model using a genetic algorithm, with a second objective function related to the structural condition of the network and the service failure risk, are presented. The basic approach is applied to three real world cases studies of sanitary sewerage systems in Coimbra and the results show the simplicity, suitability, effectiveness, and usefulness of the approximation implemented and of the objective function proposed

Environmental and Geologic Assessment to Locate a Manual Sanitary Refill in the Mene de Mauroa Church, Venezuela

Directory of Open Access Journals (Sweden)

Simón E. Morales-Soto

2016-05-01

Full Text Available The inadequate disposal of urban wastes is nowadays an environmental issue having a negative impact on several communities. The objective of this investigation is to select the most adequate place to locate a manual sanitary landfill to dump the solid wastes from the Mene de Mauroa (Venezuela church. A total of 19 variables were subjected to a series of evaluations applying the scale and weight method. This method consists of comparing the variables according to their level of priority. The area with the highest score is to be selected as the most adequate area for the sanitary landfill according to a weighting scale of 5 values for the scoring of each variable.

Veterinary-sanitary supervision of food from radiation-hygienic aspect in border crossing

International Nuclear Information System (INIS)

Vukovic, D.; Mitrovic, R.; Vicentijevic, M.; Kljajic, R.

1997-01-01

In this paper we presented the result of radiation control for imported food under the veterinary-sanitary supervision with radiation-hygienic aspect. The activity level of 137 Cs was from background to 18,8 Bq/kg and determined by gamma spectrometry. We discussed about results because 137 Cs relevance for radiation-hygienic certification and some samples of food was not satisfied maximum permitted levels. (author)

[Development of sanitary microbiology researches at the A. N. Marzeyev Institute for Hygiene and Medical Ecology, Academy of Medical Sciences of Ukraine (Kiev)].

Science.gov (United States)

Serdiuk, A M; Surmasheva, E V; Korchak, G I

2011-01-01

The paper describes the main stages of development of sanitary bacteriological studies at the leading hygiene research institute of Ukraine--the A. N Marzeyev Institute for Hygiene and Medical Ecology. These researches have made a substantial contribution to the formation and development of hygiene science in the former Soviet Union. The current and promising areas in sanitary microbiology in Ukraine are considered.

Comments on Environmental and Sanitary Aspects of the Scorpionism by Tityus trivittatus in Buenos Aires City, Argentina

Directory of Open Access Journals (Sweden)

Adolfo Rafael de Roodt

2014-04-01

Full Text Available Deaths by venomous animals are medical emergencies that can lead to death and thus constitute sanitary problems in some regions of the world. In the South of America, the accidents by these animals are a common sanitary problem especially in warm, tropical or subtropical regions, related with rural work in several countries. Argentina is located in the extreme South of South America and a minor part of the continental surface is in tropical or subtropical regions, where most of the accidents by venomous animals happen. However, in the big cities in the center and South of the country, with no relation to rural work, scorpionism, mostly due to the synanthropic and facultative parthenogenetic scorpion Tityus trivittatus, has become a sanitary problem in the last few decades. This scorpion is present in the biggest cities of Argentina and in the last decades has killed over 20 children in provinces of the center and north of the country, mostly in big cities. In addition, it seems that this species is growing and spreading in new regions of the cities. In this revision, some characteristics of this scorpion regarding its habitat, spreading in Buenos Aires city, combat measures and available treatments are discussed.

The occurrence and prevention of Infectious Pancreatic Necrosis Virus (IPNV) in rainbow trout fish farms: the importance of improved sanitary practices

OpenAIRE

AGIM REXHEPI; KURTESH SHERIFI; PETER SCHEINERT; LINDA GRAPCI-KOTORRI

2014-01-01

The viral diseases of rainbow trout are of worldwide concern including infectious pancreatic necrosis (IPN). On this study good sanitary practices are applied to prevent outbreaks and minimize losses caused from IPN disease. To highlight importance of improvements on sanitary practices in IPN prevention were compared results from two studies, first conducted in year 2006-2009 with results from one year later study conducted in 2010. In year 2010, samples of organs are collected from 260 fish ...

Flooding the sanitary city : Planning discourse and the materiality of urban sanitation in Hanoi

NARCIS (Netherlands)

Schramm, S.

2016-01-01

Urban water flows are constitutive elements of Hanoi’s morphology. Regular floods across the city illustrate that Hanoi’s amphibious character is a central impediment to the installa- tion of a ‘dry and sanitary city’, the global modernist ideal of a separation of urban waste- water flows from

Quality prediction of a leached produced in a sanitary landfills

International Nuclear Information System (INIS)

Agudelo Garcia, Ruben Alberto; Garcia R, Francisco Fernando; Rivera Mesa, Carolina

2002-01-01

This paper presents a mathematical model developed with the purpose of predicting the concentration of pollutants in the leached produced by the biological degradation of the solid wastes disposed in sanitary landfills. The model consists in a kinetic equation of first level, able to describe the degradation of the organic matter being the solid residuals. This model was calibrated using the results obtained from six laboratory scale lysimeters operated under different conditions. The model predicted the concentration of the pollutants in the leached with an accuracy of 94%

New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II

Czech Academy of Sciences Publication Activity Database

Jeníková, M.; Němejc, K.; Sak, Bohumil; Květoňová, Dana; Kváč, Martin

2011-01-01

Roč. 176, 2/3 (2011), 120-125 ISSN 0304-4017 R&D Projects: GA ČR GP523/07/P117 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cryptosporidium suis * Cryptosporidium pig genotype II * Mixed infection * Age-specificity * Species-specific primers Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.579, year: 2011

PROJECTS ON AIRPORTS, AIRFIELDS, HELICOPTER AERODROMES AND LANDING GROUNDS SANITARY PROTECTION AREAS AS THE BASIS OF THEIR ACTIVITY COMPLIANCE ASSESSMENT TO ECOLOGICAL REQUIREMENTS

Directory of Open Access Journals (Sweden)

Oleg A. Kartyshev

2017-01-01

Full Text Available Ten years experience of the development of domestic airports sanitary protection zones projects in civil aviation based on Sanitary Regulations and Norms 2.2.1/2.1.1.1200-03 requirements is analyzed, the reasons of loss of quality of project works and delaying of coordination terms at the last stage of the development are established. It is shown that among the main reasons for current negative situation with projects development and coordination are: absence of a check algorithm of settlement borders of areas and contours of aviation noise with the use of natural researches methods in Sanitary Regulations and Norms 2.2.1/2.1.1.1200-03; absence of methods of amendment in projects and regulations of establishment of the airport sanitary protection zones final dimensions. Examples of oversized and undersized noise areas and ones of the polluting substances dispersion as well as the reasons of local authorities’ interests in it are given.Conceptional strategies which should be used in the edition of a new Russian conservancy normative document regulating the development of projects on sanitary protection areas. New document will allow to consider various factors of negative environmental impact of the airports, airfields, helicopter aerodromes and landing grounds activities.It is offered to introduce the concept of "aviation noise set index operational card", which will solve the legitimation problem of the aviation noise account in the State Immovable Property Cadaster. Suggestions on equivalent and maximum level magnitude of the sound made at the residential territory of cities and urban-type settlements by flights and aircraft ground operation are given.Standards are supposed to be harmonized with similar international rules and have to provide zone gradation by 5 dBA.Suggestions on the considerations of the revealed deficiencies are made, requirements to project sections structure of sanitary protection zones are formulated, proposals on the

Genomic resources and draft assemblies of the human and porcine varieties of scabies mites, Sarcoptes scabiei var. hominis and var. suis.

Science.gov (United States)

Mofiz, Ehtesham; Holt, Deborah C; Seemann, Torsten; Currie, Bart J; Fischer, Katja; Papenfuss, Anthony T

2016-06-02

The scabies mite, Sarcoptes scabiei, is a parasitic arachnid and cause of the infectious skin disease scabies in humans and mange in other animal species. Scabies infections are a major health problem, particularly in remote Indigenous communities in Australia, where secondary group A streptococcal and Staphylococcus aureus infections of scabies sores are thought to drive the high rate of rheumatic heart disease and chronic kidney disease. We sequenced the genome of two samples of Sarcoptes scabiei var. hominis obtained from unrelated patients with crusted scabies located in different parts of northern Australia using the Illumina HiSeq. We also sequenced samples of Sarcoptes scabiei var. suis from a pig model. Because of the small size of the scabies mite, these data are derived from pools of thousands of mites and are metagenomic, including host and microbiome DNA. We performed cleaning and de novo assembly and present Sarcoptes scabiei var. hominis and var. suis draft reference genomes. We have constructed a preliminary annotation of this reference comprising 13,226 putative coding sequences based on sequence similarity to known proteins. We have developed extensive genomic resources for the scabies mite, including reference genomes and a preliminary annotation.

Remaining Sites Verification Package for the 1607-F3 Sanitary Sewer System, Waste Site Reclassification Form 2006-047

Energy Technology Data Exchange (ETDEWEB)

L. M. Dittmer

2007-04-26

The 1607-F3 waste site is the former location of the sanitary sewer system that supported the 182-F Pump Station, the 183-F Water Treatment Plant, and the 151-F Substation. The sanitary sewer system included a septic tank, drain field, and associated pipeline, all in use between 1944 and 1965. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling demonstrated that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also showed that residual contaminant concentrations are protective of groundwater and the Columbia River.

Methodology for the design of Santa Rosa de Cabal sanitary landfill, Risaralda; Metodologia para el diseno del relleno sanitario de Santa Rosa de Cabal, Risaralda

Energy Technology Data Exchange (ETDEWEB)

Tabares, J; Orozco, J

1992-07-01

In 1987 the Regional Autonomous Corporation of Risaralda, CARDER and the Risaralda Government, they signed a cooperation agreement, in order to endowing from sanitary landfill to the municipalities of the department. In the mark of this agreement it was carried out the design of Santa Rosa's sanitary landfill, that with near 50.000 inhabitants it is constituted in the third city of the department. This city generates some 25 tons/day of garbage that at the present time are heady directly to the waters of San Eugenio River. The present work contains the most important methodological aspects in the design of the sanitary landfill and some comments about the approaches ideal Vs real approaches of selection of places.

Hygienic, sanitary, physical, and functional conditions of Brazilian public school food services

OpenAIRE

Almeida,Kênia Machado de; André,Maria Cláudia Porfirio; Campos,Maria Raquel Hidalgo; Díaz,Mário Ernesto Piscoya

2014-01-01

OBJECTIVE: To verify the physical, functional, hygienic, and sanitary conditions of the food services of municipal schools located in the Brazilian Midwest region. METHODS: This is a cross-sectional study of 296 school food services conducted from February to June 2012. The food services were assessed by a semi-structured check list divided into the following sections: physical conditions, available equipment, food handlers' conduct, and food service cleaning processes and procedures. Th...

The sanitary conditions of food service establishments and food safety knowledge and practices of food handlers in bahir dar town.

Science.gov (United States)

Kibret, Mulugeta; Abera, Bayeh

2012-03-01

Lack of basic infrastructure, poor knowledge of hygiene and practices in food service establishments can contribute to outbreaks of foodborne illnesses. The aims of this study were to investigate the food safety knowledge and practices of food handlers and to assess the sanitary conditions of food service establishments in Bahir Dar town. A cross-sectional study was conducted in Bahir Dar in May 2011 and data were collected using questionnaire and observation checklist on employees' knowledge of food hygiene and their practices as well on sanitary conditions of the food service establishments The median age of the food handlers was 22 years and among the 455 subjects 99 (21.8%) have had food hygiene training. Sixty six percent of the establishments had flush toilets whereas 5.9% of the establishment had no toilet. Only 149 (33.6%) of the establishments had a proper solid waste collection receptacle and there was statistically significant association between the sanitary conditions and license status of the establishments (p=0.01). Most of all, knowledge gap in food hygiene and handling practice was observed. In addition, there was statistically significant difference between trained (professional) handlers and non-trained handlers with regard to food hygiene practices (p<0.05). While more than 50% of the handlers prepare meals ahead of the peak selling time, more than 50% of the left over was poorly managed. This study revealed poor sanitary conditions and poor food hygiene practices of handlers. Educational programs targeted at improving the attitude of food handlers and licensing and regular inspections have been recommended.

Discussion of 10 CRF 20 rules for waste of effluents in sanitary sewerage

International Nuclear Information System (INIS)

Rocha, A.C.S. da; Pina, J.L.S.

1986-12-01

An interpretation of the Code of Federal Regulations, volume 10 part 20 (10 CFR 20) from Nuclear Regulatory Commission is proposed, aiming at his easier use in waste of radioactive liquid effluents to the environment, by sanitary sewerage. The study is based on recommendations of International Commission of Radiological Protection (ICPR 2) and International Atomic Energy Agency (Basic Safety Standards for Radiation Protection). (M.A.C.) [pt

The main trends of interaction of sanitary-epidemiological teams in the system of the Russian service of disaster medicine in emergency situations

International Nuclear Information System (INIS)

Zaichenko, A.I.; Zhuravleva, V.E.; Varenikov, I.I.; Komarova, V.V.; Borisov, B.K.; Pozhidaev, A.E.; Pozhidaeva, N.V.

1995-01-01

For improvement of the system of prevention of emergency situations (ES) and for rendering emergency therapeutic-prophylactic and sanitary-epidemiological aid to population in case of ES the paper has determined and proved the first and foremost tasks which are to be settled by joint efforts of the Russian Centre on Disaster Medicine Zashchita and Scientific-practical Center of hygienic expert survey for practical perfection of the efficient forms of interaction of medical-sanitary forces engaged in work in ES. 4 refs

ANALYSIS OF REMOTE SENSING ARCHAEOLOGY ON TRAFFIC FUNCTION TRANSFORMATION OF TONGJI GRAND CANAL IN SUI AND TANG DYNASTIES

Institute of Scientific and Technical Information of China (English)

WANG Xin-yuan; HE Hui; ZHOU Ying-qiu; GAO Chao; HAN Shuang-wang

2006-01-01

In China, most directions of river flowing are from west to east. During historic period, since the water traffic played an important role, it was very important to form a cross-horizontal net of water carriage route. Canals should be dug so as to make up the lack of north-south river. Tongji Grand Canal, dug in the first year of Daye (605 A.D.) in the Sui Dynasty, was the important component of north-south system of Grand Canals in China. It promoted economic and social development of the Sui, Tang and Song dynasties (605 A.D.-1279 A.D.). As Tongji Canal (i.e.Tongji Grand Canal) flowed across the Huaibei Plain, which is aggraded by abundant mud and sand deposit resulted from the Huanghe (Yellow) River flooding, many traces (such as old channel) and human culture heritages were buried under mud-sand. Tongji Canal was silted up, and disappeared in the Jin Dynasty (1115 A.D.-1234 A.D.). From then on, there were many different stories about the flowing route of the canal in historical literature. Based on space-bone and air-bone remote sensing imagery, we attempt to search the old channel of Tongji Canal, and supplement historical record. The paper discusses transformation process of Tongji Canal's traffic function, and resumptively summarizes the reasons of the transformation, which results from synthetic function of physical geographical, political, economic, and social conditions.

Molecular Basis of Resistance to Selected Antimicrobial Agents in the Emerging Zoonotic Pathogen Streptococcus suis.

Science.gov (United States)

Gurung, Mamata; Tamang, Migma Dorji; Moon, Dong Chan; Kim, Su-Ran; Jeong, Jin-Ha; Jang, Geum-Chan; Jung, Suk-Chan; Park, Yong-Ho; Lim, Suk-Kyung

2015-07-01

Characterization of 227 Streptococcus suis strains isolated from pigs during 2010 to 2013 showed high levels of resistance to clindamycin (95.6%), tilmicosin (94.7%), tylosin (93.8%), oxytetracycline (89.4%), chlortetracycline (86.8%), tiamulin (72.7%), neomycin (70.0%), enrofloxacin (56.4%), penicillin (56.4%), ceftiofur (55.9%), and gentamicin (55.1%). Resistance to tetracyclines, macrolides, aminoglycosides, and fluoroquinolone was attributed to the tet gene, erm(B), erm(C), mph(C), and mef(A) and/or mef(E) genes, aph(3')-IIIa and aac(6')-Ie-aph(2″)-Ia genes, and single point mutations in the quinolone resistance-determining region of ParC and GyrA, respectively. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

From observation to action: Ethnography of and in homelessness services

Directory of Open Access Journals (Sweden)

Valentina Porcellana

2017-12-01

Full Text Available L’articolo intende analizzare le politiche messe in atto nella (e dalla città di Torino nell’ambito della grave emarginazione adulta, le trasformazioni che hanno coinvolto i servizi di accoglienza dagli anni Ottanta a oggi e le conseguenti ricadute su operatori e utenti. Intende inoltre presentare gli esiti di una lunga esperienza di campo che, a partire dal 2009, ha portato antropologi e designer a entrare nel sistema dei servizi per comprenderlo e per proporre delle modifiche dall’interno. Una “partecipazione osservante” dei sistemi che consente di svelare il loro funzionamento e i loro significati e, contemporaneamente, di presidiare i servizi pubblici attraverso gli strumenti critici dell’antropologia politica e delle politiche del welfare.

A data bank about wild rodent specimens of Italy / Una banca dati sui roditori selvatici d'Italia

Directory of Open Access Journals (Sweden)

Giovanni Amori

1987-07-01

Full Text Available Abstract The setting up of a "data bank" dealing with the findings of Rodents specimens (Families: Gliridae, Arvicolidae, Muridae of peninsular and insular Italy available for 1950-1981 years in the italian museums and collections. The file, wherever possible, has been filled up with cartographic, bioclimatic and synanthropic data. With the use of a microcomputer has thus become possible to make inquires related both to biological and environmental data. Data management allows the following function: file maintenance (insert, delete and modify record; cross - reference search; search index; report generation. In the first phase challenges have been formulated, requiring simple data processing. Some informations were obtained as reply about the prevalences in the capturing referred to sex and season, as well as the connections between Rodents coenosis and bioclimatic condition of the territory. Riassunto È stata avviata una "banca dati" dei rinvenimenti di esemplari di Roditori (Famiglie: Gliridae, Arvicolidae, Muridae dell'Italia peninsulare ed insulare catalogati negli anni 1950-1981 nei Musei e collezioni italiani. L'archivio, laddove possibile, è stato completato con i dati cartografici, bioclimatici e sinantropici. L'uso del microcomputer ha permesso di formulare domande incrociate sia sui dati biologici che su quelli ambientali. La gestione dei dati consente le seguenti funzioni: gestione dell'archivio (inserimento, cancellazione e modifica del record; ricerca incrociata; ricerca per indice; stampa. In una prima fase sono state formulate alcune domande richiedenti semplici elaborazioni sulle preponderanze delle catture in rapporto al sesso ed alla stagione, nonché sui rapporti tra cenosi a Roditori ed assetto bioclimatico del territorio.

Menstruation and the Cycle of Poverty: A Cluster Quasi-Randomised Control Trial of Sanitary Pad and Puberty Education Provision in Uganda.

Science.gov (United States)

Montgomery, Paul; Hennegan, Julie; Dolan, Catherine; Wu, Maryalice; Steinfield, Laurel; Scott, Linda

2016-01-01

Poor menstrual knowledge and access to sanitary products have been proposed as barriers to menstrual health and school attendance. In response, interventions targeting these needs have seen increasing implementation in public and private sectors. However, there has been limited assessment of their effectiveness. Assess the impact of providing reusable sanitary pads and puberty education on girls' school attendance and psychosocial wellbeing outcomes. A cluster quasi-randomised controlled trial was conducted across 8 schools, including 1124 girls, in rural Uganda. Schools were allocated to one of four conditions: the provision of puberty education alone; reusable sanitary pads alone; puberty education and reusable sanitary pads; and a control (no intervention). The primary outcome was school attendance. Secondary outcomes reflected psychosocial wellbeing. At follow-up, school attendance had worsened for girls across all conditions. Per-protocol analysis revealed that this decline was significantly greater for those in the control condition d = 0.52 (95%CI 0.26-0.77), with those in control schools having a 17.1% (95%CI: 8.7-25.5) greater drop in attendance than those in any intervention school. There were no differences between the intervention conditions. High rates of school drop-out and transfer meant the trial suffered from substantial participant drop-out. Intention-to-treat analyses using two different imputation strategies were consistent with the main results, with mean differences of 5.2% attendance in best-case and 24.5% in worst-case imputations. Results were robust to adjustments for clustering. There was no impact of the interventions on girls' self-reported shame or insecurity during menstruation. Results of the trial support the hypothesised positive impact of providing sanitary pads or puberty education for girls' school attendance in a developing country context. Findings must be interpreted with caution in light of poor participant retention

Menstruation and the Cycle of Poverty: A Cluster Quasi-Randomised Control Trial of Sanitary Pad and Puberty Education Provision in Uganda.

Directory of Open Access Journals (Sweden)

Paul Montgomery

Full Text Available Poor menstrual knowledge and access to sanitary products have been proposed as barriers to menstrual health and school attendance. In response, interventions targeting these needs have seen increasing implementation in public and private sectors. However, there has been limited assessment of their effectiveness.Assess the impact of providing reusable sanitary pads and puberty education on girls' school attendance and psychosocial wellbeing outcomes.A cluster quasi-randomised controlled trial was conducted across 8 schools, including 1124 girls, in rural Uganda. Schools were allocated to one of four conditions: the provision of puberty education alone; reusable sanitary pads alone; puberty education and reusable sanitary pads; and a control (no intervention. The primary outcome was school attendance. Secondary outcomes reflected psychosocial wellbeing.At follow-up, school attendance had worsened for girls across all conditions. Per-protocol analysis revealed that this decline was significantly greater for those in the control condition d = 0.52 (95%CI 0.26-0.77, with those in control schools having a 17.1% (95%CI: 8.7-25.5 greater drop in attendance than those in any intervention school. There were no differences between the intervention conditions. High rates of school drop-out and transfer meant the trial suffered from substantial participant drop-out. Intention-to-treat analyses using two different imputation strategies were consistent with the main results, with mean differences of 5.2% attendance in best-case and 24.5% in worst-case imputations. Results were robust to adjustments for clustering. There was no impact of the interventions on girls' self-reported shame or insecurity during menstruation.Results of the trial support the hypothesised positive impact of providing sanitary pads or puberty education for girls' school attendance in a developing country context. Findings must be interpreted with caution in light of poor participant

Menstruation and the Cycle of Poverty: A Cluster Quasi-Randomised Control Trial of Sanitary Pad and Puberty Education Provision in Uganda

Science.gov (United States)

Montgomery, Paul; Hennegan, Julie; Dolan, Catherine; Wu, Maryalice; Steinfield, Laurel; Scott, Linda

2016-01-01

Background Poor menstrual knowledge and access to sanitary products have been proposed as barriers to menstrual health and school attendance. In response, interventions targeting these needs have seen increasing implementation in public and private sectors. However, there has been limited assessment of their effectiveness. Objectives Assess the impact of providing reusable sanitary pads and puberty education on girls’ school attendance and psychosocial wellbeing outcomes. Methods A cluster quasi-randomised controlled trial was conducted across 8 schools, including 1124 girls, in rural Uganda. Schools were allocated to one of four conditions: the provision of puberty education alone; reusable sanitary pads alone; puberty education and reusable sanitary pads; and a control (no intervention). The primary outcome was school attendance. Secondary outcomes reflected psychosocial wellbeing. Results At follow-up, school attendance had worsened for girls across all conditions. Per-protocol analysis revealed that this decline was significantly greater for those in the control condition d = 0.52 (95%CI 0.26–0.77), with those in control schools having a 17.1% (95%CI: 8.7–25.5) greater drop in attendance than those in any intervention school. There were no differences between the intervention conditions. High rates of school drop-out and transfer meant the trial suffered from substantial participant drop-out. Intention-to-treat analyses using two different imputation strategies were consistent with the main results, with mean differences of 5.2% attendance in best-case and 24.5% in worst-case imputations. Results were robust to adjustments for clustering. There was no impact of the interventions on girls’ self-reported shame or insecurity during menstruation. Conclusion Results of the trial support the hypothesised positive impact of providing sanitary pads or puberty education for girls’ school attendance in a developing country context. Findings must be interpreted

Municipal solid wastes (RSU) treatment in land filled controlled: Of its sanitary problems to energy production

International Nuclear Information System (INIS)

Urzola C, R.A.

1995-01-01

Excessive world wastes production generates constant preoccupations with relation to its management. The methods of classic elimination as sanitary landfill controlled as compared to controvertible incineration and gasification plants as well as expectations created in connection with practices of recycling, its are intensive discussions motive between town halls, technical specialists, ecologists and general public. What is certain is that wastes in the last 30 years have been valued economically. Today the wastes are appreciated by certain sectors that begin to monopolize the recycling line because they see an excellent business. All methods of residues elimination produce at same time other wastes. Solid nature wastes generally end in the landfill and semi-liquid waste are dehydrated with same objective. In the present Thesis, without intending to say that the landfill processing will be the better option, is outlined the form of optimizing the utilization of same. It's demonstrated that landfill, furthermore to fulfil its sanitary function, are converted into an energetic alternative with utilization of flammable gas generated in them, something which represents an interesting value added if is converted into source of welfare for social and economically kernels, as happens in peripheries of the sanitary landfill at large Latin American cities. Of other side are considered principal aspects that intervene in methane gas generation and quality and utilization alternatives. Finally it is made emphasis in environmental situation at European and international level about the landfill gases, how is outlined greenhouse effect topic, what are environmental actions for year 2000 and are related some biogas utilization experiences at Europe

Extra environmental impact assessment and recommendable measures for their minimization in arranging sanitary inspection room site

International Nuclear Information System (INIS)

Rud'ko, V.M.; Batij, V.G.; Kuz'menko, V.A.; Paskevich, S.A.

2003-01-01

Environmental impact assessments during the works for preparation of sanitary inspection room site are presented. A range of measures to minimize environmental impact from the works to be implemented,is offered. Impacts on such environmental components as soil,air and aqueous medium,are considered

Hume, Mill, Hill, and the sui generis epidemiologic approach to causal inference.

Science.gov (United States)

Morabia, Alfredo

2013-11-15

The epidemiologic approach to causal inference (i.e., Hill's viewpoints) consists of evaluating potential causes from the following 2, noncumulative angles: 1) established results from comparative, observational, or experimental epidemiologic studies; and 2) reviews of nonepidemiologic evidence. It does not involve statements of statistical significance. The philosophical roots of Hill's viewpoints are unknown. Superficially, they seem to descend from the ideas of Hume and Mill. Hill's viewpoints, however, use a different kind of evidence and have different purposes than do Hume's rules or Mill's system of logic. In a nutshell, Hume ignores comparative evidence central to Hill's viewpoints. Mill's logic disqualifies as invalid nonexperimental evidence, which forms the bulk of epidemiologic findings reviewed from Hill's viewpoints. The approaches by Hume and Mill cannot corroborate successful implementations of Hill's viewpoints. Besides Hume and Mill, the epidemiologic literature is clueless about a plausible, pre-1965 philosophical origin of Hill's viewpoints. Thus, Hill's viewpoints may be philosophically novel, sui generis, still waiting to be validated and justified.

Hygienic, sanitary, physical, and functional conditions of Brazilian public school food services

Directory of Open Access Journals (Sweden)

Kênia Machado de Almeida

2014-06-01

Full Text Available OBJECTIVE: To verify the physical, functional, hygienic, and sanitary conditions of the food services of municipal schools located in the Brazilian Midwest region. METHODS: This is a cross-sectional study of 296 school food services conducted from February to June 2012. The food services were assessed by a semi-structured check list divided into the following sections: physical conditions, available equipment, food handlers' conduct, and food service cleaning processes and procedures. The study variables were classified as compliant or noncompliant with the regulations passed by the National Sanitary Surveillance Agency. RESULTS: Noncompliances were found in all study food services, especially with respect to food service conditions, and the wiring and plumbing in the food preparation area. In this section, 62.7 to 95.9% of the food services did not comply with nine out of the thirteen study items. The main problems were: poorly cleaned external areas, deteriorated walls, floors, ceilings, roofs, drains, and roof gutters; and unscreened doors and windows, allowing the entrance of insects; among others. The main noncompliance regarding processes and procedures was the uncontrolled temperature of the ready-to-eat foods. CONCLUSION: The conditions of the study food services are unsatisfactory for the production of safe meals, possibly compromising meal quality, food safety, and the effectiveness of the School Food Program.

[The information on a sanitary-and-epidemiologic condition of general educational establishments and catering services of schoolboys].

Science.gov (United States)

Onishchenko, G G

2008-01-01

The federal service on supervision in sphere of protection of the rights of consumers and well-being of the person develops normative and methodical documents, including sanitary rules and the norms defining hygienic parameters of food value of food raw material and foodstuff, children used in a feed and teenagers; requirements to catering services of pupils of various types of teaching and educational establishments. Decisions of the Main state health officer of the Russian Federation, the conditions directed on improvement and catering services in educational establishments are published. At participation of Rospotrebnadzor's experts on subjects of the Russian Federation the regional programs directed on improvement of catering services of pupils are developed. The information on a condition of general educational establishments with offers on improvement of a sanitary-engineering condition, goes to address of enforcement authorities.

Evaluation of sanitary consequences of Chernobylsk accident in France. Epidemiological surveillance plan, state of knowledge, risks evaluation and perspectives

International Nuclear Information System (INIS)

Verger, P.; Cherie-Challine, L.

2000-12-01

This report jointly written by IPSN and InVS, reviews the sanitary consequences in France of the Chernobyl accident, which occurred in 1986. The first point is dedicated to a short presentation of the knowledge relative to the sanitary consequences of the Chernobyl accident in the high contaminated countries and to the risk factors of the thyroid cancer. Secondly, this report describes the main systems of epidemiological surveillance of health implemented in France in 1986 and in 1999, as well as the data of the incidence and mortality of thyroid cancer observed in France since 1975. In addition, this report presents an analysis of the risk of thyroid cancer related to radioactive contamination in France, for young people of less than 15 years of age who where living in 1986 in the highest contaminated areas of France (Eastern territories). For this purpose, the theoretical number of thyroid cancers in excess is evaluated for this population, on the basis of different available risk model. Finally starting from the results of risk assessment, there is a discussion about the relevance and the feasibility of different epidemiological methods in view of answering the questions related to the sanitary consequences of the Chernobyl accident. In conclusion, this report recommends to reinforce the surveillance of thyroid cancer in France. (author)

Evaluation of the sanitary impact of urban air pollution. Update version of the methodological guide; Evaluation de l'impact sanitaire de la pollution atmospherique urbaine. Version actualisee du guide methodologique

Energy Technology Data Exchange (ETDEWEB)

NONE

2003-03-15

In order to bring a help to the evaluation of effects of air quality on health, the Institute of Sanitary surveillance published in 1999 a method guide for the realisation of sanitary impact evaluations of the urban air pollution. This work to prepare to an update version of the guide presents the first recommendations that can be made today for the realisation of sanitary impact evaluations at short and long term at a local level, taking into account the knowledge evolution. (N.C.)

Identification and characterization of two temperature-induced surface-associated proteins of Streptococcus suis with high homologies to members of the arginine deiminase system of Streptococcus pyogenes

NARCIS (Netherlands)

Winterhoff, N.; Goethe, R.; Gruening, P.; Rohde, M.; Kalisz, H.; Smith, H.E.; Valentin-Weigand, P.

2002-01-01

The present study was performed to identify stress-induced putative virulence proteins of Streptococcus suis. For this, protein expression patterns of streptococci grown at 32, 37, and 42°C were compared by one- and two-dimensional gel electrophoresis. Temperature shifts from 32 and 37 to 42°C

Conceptual model elaboration for the safety assessment of phosphogypsum use in sanitary landfills

International Nuclear Information System (INIS)

Cota, Stela D.; Braga, Leticia T.P.; Jacomino, Vanusa F.

2009-01-01

Phosphogypsum is a by-product of the phosphatic fertilizer production from the beneficiation of phosphate minerals (apatites). Produced in large quantities throughout the world and stored temporally in stacks, the final destination of this product is nowadays a subject of investigation. Due to the presence of radionuclides ( 226 Ra, 232 Th and 40 K, mainly), possible applications for the phosphogypsum must be verified for radiological safety. The goal of this paper was to elaborate a representative water flow conceptual model of a sanitary landfill for the safety assessment of the impact of using phosphogypsum as a cover material. For this, the ground water flow in variably saturated conditions and solute transport model HYDRUS-2D has been used for simulating the impact in the saturated zone of potential radionuclides leaching. The conceptual model was developed by collecting and analyzing the data from environmental license documentation of municipal sanitary landfills located on the State of Minas Gerais, Brazil. In order to fulfill the requirements of HDRUS-2D model in terms of the necessary parameters, the physical characteristics and typical configuration of the landfills, as well as the hydrogeological parameters of soils and aquifers related to the local of placement of the landfills, were taken in account for the formulation of the conceptual model. (author)

76 FR 35215 - Notice of EPA Workshop on Sanitary Sewer Overflows and Peak Wet Weather Discharges

Science.gov (United States)

2011-06-16

... maintained sanitary sewer systems are meant to collect and transport all of the sewage that flows into them... sewage from these overflows can contaminate our waters, causing serious water quality problems. It can... influent flows to exceed the treatment capacity of existing secondary treatment units. Known as ``peak...

76 FR 34145 - Safety Zone, Barrier Testing Operations, Chicago Sanitary and Ship Canal, Romeoville, IL

Science.gov (United States)

2011-06-13

...-AA00 Safety Zone, Barrier Testing Operations, Chicago Sanitary and Ship Canal, Romeoville, IL AGENCY.... Construction on Barrier IIB has been completed. Operational and safety testing was conducted in February 2011... dispersal barrier IIA and IIB. This safety zone will be enforced daily from 7 a.m. to 11 a.m. and from 1 p.m...

INVESTIGATION OF SANITARY-HYGIENIC CHARACTERISTICS OF MULTILAYER POLYMER FILMS USED FOR VACUUM PACKAGING MODIFIED BY NATIVE ANTIMICROBIAL COMPONENTS

Directory of Open Access Journals (Sweden)

O. B. Fedotova

2016-01-01

Full Text Available The results of the research works related to investigation of sanitary-hygienic characteristics of multilayer polymer film materials where the inner layer contacting directly with food product is modified by native antimicrobial components.

PREPARATIVE ISOLATION AND PURIFICATION OF THREE GLYCINE-CONJUGATED CHOLIC ACIDS FROM PULVIS FELLIS SUIS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY COUPLED WITH ELSD DETECTION

OpenAIRE

He, Jiao; Li, Jing; Sun, Wenji; Zhang, Tianyou; Ito, Yoichiro

2012-01-01

Coupled with evaporative light scattering detection, a high-speed counter-current chromatography (HSCCC) method was developed for preparative isolation and purification of three glycine-conjugated cholic acids, glycochenodeoxycholic acid (GCDCA), glycohyodeoxycholic acid (GHDCA) and glycohyocholic acid (GHCA) from Pulvis Fellis Suis (Pig gallbladder bile) for the first time. The separation was performed with a two-phase solvent system consisted of chloroform-methanol-water-acetic acid (65:30:...

Je suis un zombie : Études récentes sur Richard Matheson et le mort-vivant

OpenAIRE

Ransom, Amy J.; Trudel, Jean-Louis

2013-01-01

Dans ce texte nous résumons quatre essais récents sur le phénomène actuel du zombie ainsi qu’un collectif sur l’œuvre de Richard Matheson dont la première version filmique du roman Je suis une légende a servi d’inspiration à l’ur-film de zombies, La nuit des morts vivants de George A. Romero. Tandis que Pierre Cassou-Noguès et Maxime Coulombe signent des traitements plus philosophiques du zombie, leurs ouvrages diffèrent beaucoup dans leur forme et leur fonction. Pour leur part, Amélie Pépin ...

Semi-Preparative Isolation and Purification of Three Tauro-Conjugated Cholic Acids from Pulvis Fellis Suis by HSCCC Coupled with ELSD Detection

OpenAIRE

He, Jiao; Zhang, Yongmin; Ito, Yoichiro; Sun, Wenji

2011-01-01

Coupled with evaporative light scattering detection, a high-speed counter-current chromatography (HSCCC) method was applied to the separation and purification of three tauro-conjugated cholic acids of taurochenodeoxycholic acid (TCDCA), taurohyodeoxycholic acid (THDCA) and taurohyocholic acid (THCA) from Pulvis Fellis Suis (Pig gallbladder bile) for the first time. The two-phase solvent system composed of chloroform-methanol-water-acetic acid (4:4:2:0.3, v/v/v/v) was selected for the one-step...

Erika: evaluation of residual sanitary risks linked to the beaches-going after cleaning-up; Erika: evaluation des risques sanitaires residuels lies a la frequentation des plages apres depollution

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-07-01

The aim of this study is to evaluate the sanitary risks for the populations frequenting the coast beaches affected by the fuel oils of the tanker Erika, after their cleaning up. This quantitative evaluation of sanitary risks is led according to a protocol developed jointly between the two institutes ( the national institute of industrial environment and risks, I.N.E.R.I.S., and the national institute of sanitary watch I.n.V.S.). It does not give the risks in function the residual pollution level of concerned beaches but supplies to the authorities elements of scientific information in order to allow them to choose criteria appropriate for the management of eventual residual risks. (N.C.)

Crystallization and preliminary crystallographic analysis of recombinant immunoglobulin G-binding protein from Streptococcus suis

Energy Technology Data Exchange (ETDEWEB)

Khan, Abdul Hamid; Chu, Fuliang; Feng, Youjun; Zhang, Qinagmin [Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101 (China); Qi, Jianxun [Institute of Physics, Chinese Academy of Sciences, Beijing 100080 (China); Gao, George Fu, E-mail: gaof@im.ac.cn [Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101 (China)

2008-08-01

Crystallization of recombinant IgG-binding protein expressed in Escherichia coli using the hanging-drop vapour-diffusion method is described. The crystals belonged to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 38.98, b = 43.94, c = 78.17 Å. Streptococcus suis, an important zoonotic pathogen, expresses immunoglobulin G-binding protein, which is thought to be helpful to the organism in eluding the host defence system. Recombinant IgG-binding protein expressed in Escherichia coli has been crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 38.98, b = 43.94, c = 78.17 Å and one molecule in the asymmetric unit. Diffraction data were collected to 2.60 Å resolution.

Evaluation of new location of Isfahan′s sanitary landfill site with Oleckno method

OpenAIRE

Maryam Salimi; Afshin Ebrahimi; Afsane Salimi

2013-01-01

Aims: The objective of present study was to evaluate the new location of Isfahan solid waste sanitary landfill using Geographical Information System (GIS) based on the Oleckno index method (OIM). Materials and Methods: This study was on the field- and library-based data collection and surveys of relevant data. Assessment parameters included average annual rainfall, soil type and ground water beneath and adjucent to the landfill site. To analyze data, ArcGIS version 9.3 was used. Resul...

Do sanitary ceramic workers have a worse presentation of chest radiographs or pulmonary function tests than other ceramic workers?

Directory of Open Access Journals (Sweden)

Yu-Chung Tsao

2017-03-01

Conclusion: In this study, we found that sanitary ceramic workers were at a similar risk to other ceramic workers for moderate to severe silicosis when older age and longer working duration were accounted for.

[Sanitary control of alcohol advertisement in Brazil: a study of the law bills from 1988 to 2004].

Science.gov (United States)

Falcão, Isa Cristina Lopes; Rangel-S, Maria Ligia

2010-11-01

This study analyses law bills towards the sanitary control of alcohol advertisement in the mass media presented to the Brazilian Congress from 1988 to 2004. The sanitary control of this advertising is a controversial issue bringing about an ethical-political debate in which the health-protecting interests conflict with commercial ones even after scientific evidence has established increasing alcohol intake as a health and social issue worldwide. The content analysis of 67 of such law bills was carried out, and these proposals were shown to consist mostly of strategies to both cope with alcohol advertising by dissociating from the product those values that might interfere with the children and teenagers' building of their social identity and limit advertisement contents in the mass media given the higher vulnerability of the subjects in that age range. This study reveals complexes challenges for the control of alcohol advertisement that in turn lead to rethinking the action of the State apparatus in dealing with this major public health issue.

SOIL AND “CERRADO” TREES NUTRIENTS AND METALS IN ADJACENT SANITARY LANDFILL AREA

OpenAIRE

Otacílio Antunes Santana; José Imanã Encinas; Rodrigo Studart Corrêa; Antônio Felipe Couto Júnior

2008-01-01

This research verified the influence of a Sanitary Landfill located at the Jockey Club of the Brasilia City (JCB) on the chemical contents in the tree species of “Cerrado”. Six 25 x 500 m blocks were established in the PNB to sample the soil and the trees to chemical analysis. Three blocks were established near the landfill area and three in the control area. Nitrogen, phosphorus, potassium, calcium, lead, chromium, copper and mercury were analyzed. The highest nutrients and metals concentrat...

Sanitary landfill leachate as a source of nutrients on the initial growth of sunflower plants

Directory of Open Access Journals (Sweden)

Francisco H. Nunes Júnior

Full Text Available ABSTRACT The aim of this study was to evaluate the initial growth of sunflower seedlings under different concentrations of sanitary landfill leachate, considering the feasibility of its use as source of nutrients for agricultural production. Biometric and vigor variables were analyzed through the measurements of collar diameter, shoot height, number of leaves and shoot and root fresh and dry matters, from January to February 2015. The experimental design was completely randomized in a 5 x 4 factorial scheme: five leachate concentrations (0, 40, 60, 80 and 100 kg N ha-1 x four harvest periods (14, 21, 25 and 29 days after sowing, with five replicates each containing two plants. The data were subjected to analysis of variance and polynomial regression, and the results of the last harvest (29 DAS were compared by Tukey test (p ≤ 0.05. The use of sanitary landfill leachate increased all analyzed variables in sunflower plants when compared to the control plants (without leachate, especially in the treatment of 100 kg N ha-1. There was no inhibitory effect of the leachate on the initial growth of sunflower seedlings under adopted experimental conditions.

77 FR 65760 - Request for Public Comments To Compile the Reports on Sanitary and Phytosanitary and Technical...

Science.gov (United States)

2012-10-30

... Technical Barriers to Trade (2012 TBT Report) respectively. The TPSC invites written comments from the public on issues that USTR should examine in preparing the SPS and TBT Reports. DATES: Public comments..., Director of Sanitary and Phytosanitary Affairs, USTR (202-395-6127). Questions regarding the TBT Report or...

Phyto sanitary Treatment at SINAGAMA: Experience Sharing and Challenge

International Nuclear Information System (INIS)

Sofian Ibrahim; Jamilah Karim; Mohd Sidek Othman; Ahsanulkhaliqin Abdul Wahab; Ahmad Zainuri Mohd Dzomir; Zainon Othman; Hasan Sham; Mohd Khairul Azfar Ramli

2012-01-01

After more than 22 years involved in sterilization and decontamination of products like medical devices, foods and herbs, Sinagama with the cooperation of SSDL, BAB, MARDI and Malaysia Department of Agriculture has make another step ahead by helping the local fruits farmers on phyto sanitary treatment in order to help them exporting their crops to United States. This report was prepared to discuss the challenges that have been faced by Sinagama during registration and certification process from United States Department of Agriculture (USDA). The challenges that Sinagama have to faced include Minimum Irradiation Dose, Dose Mapping Process, Biological Control, Training Records, Working Procedures and requirements that needed by Plant Health Inspection Service (APHIS) Officer. All these challenged has been faced by Sinagama in order to make sure the ambition of our nation to help farming sector become reality. (author)

75 FR 36288 - Amended Safety Zone and Regulated Navigation Area, Chicago Sanitary and Ship Canal, Romeoville, IL

Science.gov (United States)

2010-06-25

...The Coast Guard is revising its safety zone and Regulated Navigation Area (RNA) on the Chicago Sanitary and Ship Canal (CSSC) near Romeoville, IL. This revised temporary interim rule reduces the areas covered by the safety zone and RNA, and places additional restrictions on vessels that may transit the RNA.

Identity And Access Management e Federazione

Directory of Open Access Journals (Sweden)

Virginia Calabritto

2012-07-01

Full Text Available ItLa rete telematica è in costante crescita e così i servizi online che, in modo sempre più penetrante, rappresentano un aspetto della nostra quotidianità. I servizi in rete si distinguono in servizi ad accesso libero e servizi ad accesso controllato. Ogni giorno studenti, ricercatori e docenti accedono a contenuti e servizi (risorse ad accesso controllato con modalità diverse, la più diffusa è l'utilizzo dell‘informazione utenza/password, informazione diversa per ciascuna risorsa. Da questo punto di vista l’ampia disponibilità di risorse in rete crea all’utente, ma non solo a lui, diversi problemi, non ultimo quello della memorizzazione di un numero sempre più ampio di coppie utenza/password.L’articolo è teso ad illustrare, nel dettaglio, scenario e problematiche per passare quindi ad accennare alle tecnologie che possono costituire una soluzione, ovvero, l'Identity Management, il Single Sign On e le federazioni di infrastrutture di autenticazione e autorizzazione e, quindi, a degli esempi tra cui IDEM, la prima federazione italiana di Infrastrutture di Autenticazione e Autorizzazione, che coinvolge gli enti della comunità scientifica ed accademica e i fornitori di servizi.EnThe Internet is constantly growing, therefore on-line services, are becoming part of our daily life in a more and more pervasive manner. The network services are divided into two types of access: free access and controlled access. Every day students, researchers and teachers access content and services (resources through controlled access using different modes, e.g using user / password information which is different for each resource. From this perspective, the large availability of networked resources generates many problems for the users, last but not least the inconvenience of storing an increasing number of user / password.The article aims to illustrate, in detail, the context and the problems related to the management of a digital identiy in the

Remaining Sites Verification Package for the 1607-F4 Sanitary Sewer System. Attachment to Waste Site Reclassification Form 2004-131

International Nuclear Information System (INIS)

Dittmer, L.M.

2007-01-01

The 1607-F4 waste site is the former location of the sanitary sewer system that serviced the former 115-F Gas Recirculation Building. The system included a septic tank, drain field, and associated pipeline that were in use from 1944 to 1965. The 1607-F4 waste site received unknown amounts of sanitary sewage from the 115-F Gas Recirculation Building and may have potentially contained hazardous and radioactive contamination. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling demonstrated that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also showed that residual contaminant concentrations are protective of groundwater and the Columbia River

Adhesion activity of glyceraldehyde-3-phosphate dehydrogenase in a Chinese Streptococcus suis type 2 strain.

Science.gov (United States)

Wang, Kaicheng; Lu, Chengping

2007-01-01

A total of 36 streptococcal strains, including seven S. equi ssp.zooepidemicus, two S. suis type 1 (SS1), 24 SS2, two SS9, and one SS7, were tested for glyceraldehyde-3-phosphate dehydrogenase gene (gapdh). Except from non-virulent SS2 strain T1 5, all strains harboured gapdh. The gapdh of Chinese Sichuan SS2 isolate ZY05719 and Jiangsu SS2 isolate HA9801 were sequenced and then compared with published sequences in the GenBank. The comparison revealed a 99.9 % and 99.8 % similarity of ZY05719 and HA9801, respectively, with the published sequence. Adherence assay data demonstrated a significant ((p<0.05)) reduction in adhesion of SS2 in HEp-2 cells pre-incubated with purified GAPDH compared to non pre-incubated controls, suggesting the GAPDH mediates SS2 bacterial adhesion to host cells.

Flooding the sanitary city : Planning discourse and the materiality of urban sanitation in Hanoi

OpenAIRE

Schramm, S.

2016-01-01

Urban water flows are constitutive elements of Hanoi’s morphology. Regular floods across the city illustrate that Hanoi’s amphibious character is a central impediment to the installa- tion of a ‘dry and sanitary city’, the global modernist ideal of a separation of urban waste- water flows from public space through their redirection into large underground networks. Currently, the first attempt by the city government to construct a citywide sewerage network since the colonial period is taking p...

Remaining Sites Verification Package for the 100-F-26:10, 1607-F3 Sanitary Sewer Pipelines (182-F, 183-F, and 151-F Sanitary Sewer Lines), Waste Site Reclassification Form 2007-028

Energy Technology Data Exchange (ETDEWEB)

L. M. Dittmer

2007-12-03

The 100-F-26:10 waste site includes sanitary sewer lines that serviced the former 182-F, 183-F, and 151-F Buildings. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling show that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also demonstrate that residual contaminant concentrations are protective of groundwater and the Columbia River.

Secondary infection with Streptococcus suis serotype 7 increases the virulence of highly pathogenic porcine reproductive and respiratory syndrome virus in pigs.

Science.gov (United States)

Xu, Min; Wang, Shujie; Li, Linxi; Lei, Liancheng; Liu, Yonggang; Shi, Wenda; Wu, Jiabin; Li, Liqin; Rong, Fulong; Xu, Mingming; Sun, Guangli; Xiang, Hua; Cai, Xuehui

2010-08-09

Porcine reproductive and respiratory syndrome virus (PRRSV) and Streptococcus suis are common pathogens in pigs. In samples collected during the porcine high fever syndrome (PHFS) outbreak in many parts of China, PRRSV and S. suis serotype 7 (SS7) have always been isolated together. To determine whether PRRSV-SS7 coinfection was the cause of the PHFS outbreak, we evaluated the pathogenicity of PRRSV and/or SS7 in a pig model of single and mixed infection. Respiratory disease, diarrhea, and anorexia were observed in all infected pigs. Signs of central nervous system (CNS) disease were observed in the highly pathogenic PRRSV (HP-PRRSV)-infected pigs (4/12) and the coinfected pigs (8/10); however, the symptoms of the coinfected pigs were clearly more severe than those of the HP-PRRSV-infected pigs. The mortality rate was significantly higher in the coinfected pigs (8/10) than in the HP-PRRSV- (2/12) and SS7-infected pigs (0/10). The deceased pigs of the coinfected group had symptoms typical of PHFS, such as high fever, anorexia, and red coloration of the ears and the body. The isolation rates of HP-PRRSV and SS7 were higher and the lesion severity was greater in the coinfected pigs than in monoinfected pigs. HP-PRRSV infection increased susceptibility to SS7 infection, and coinfection of HP-PRRSV with SS7 significantly increased the pathogenicity of SS7 to pigs.

Secondary infection with Streptococcus suis serotype 7 increases the virulence of highly pathogenic porcine reproductive and respiratory syndrome virus in pigs

Directory of Open Access Journals (Sweden)

Xu Min

2010-08-01

Full Text Available Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV and Streptococcus suis are common pathogens in pigs. In samples collected during the porcine high fever syndrome (PHFS outbreak in many parts of China, PRRSV and S. suis serotype 7 (SS7 have always been isolated together. To determine whether PRRSV-SS7 coinfection was the cause of the PHFS outbreak, we evaluated the pathogenicity of PRRSV and/or SS7 in a pig model of single and mixed infection. Results Respiratory disease, diarrhea, and anorexia were observed in all infected pigs. Signs of central nervous system (CNS disease were observed in the highly pathogenic PRRSV (HP-PRRSV-infected pigs (4/12 and the coinfected pigs (8/10; however, the symptoms of the coinfected pigs were clearly more severe than those of the HP-PRRSV-infected pigs. The mortality rate was significantly higher in the coinfected pigs (8/10 than in the HP-PRRSV- (2/12 and SS7-infected pigs (0/10. The deceased pigs of the coinfected group had symptoms typical of PHFS, such as high fever, anorexia, and red coloration of the ears and the body. The isolation rates of HP-PRRSV and SS7 were higher and the lesion severity was greater in the coinfected pigs than in monoinfected pigs. Conclusion HP-PRRSV infection increased susceptibility to SS7 infection, and coinfection of HP-PRRSV with SS7 significantly increased the pathogenicity of SS7 to pigs.

Sanitary Landfill groundwater monitoring report. Fourth quarterly report and summary 1993

Energy Technology Data Exchange (ETDEWEB)

1994-02-01

Fifty-seven wells of the LFW series monitor groundwater quality in Steed Pond Aquifer (Water Table) beneath the Sanitary Landfill at the Savannah River Site (SRS). These wells are sampled quarterly to comply with the South Carolina Department of Health and Environmental Control Domestic Waste Permit DWP-087A and as part of the SRS Groundwater Monitoring Program. Dichloromethane a common laboratory contaminant, and trichloroethylene were the most widespread constituents exceeding standards during 1993. Benzene, chlorobenzene, chloroethene 1,2 dichloroethane, 1,1-dichloroethylene, 1,2-dichloropropane, gross alpha, lindane, mercury, tetrachloroethylene, and tritium also exceeded standards in one or more wells. No groundwater contaminants were observed in wells screened in the lower section of Steed Pond Aquifer.

[The primary medical sanitary care and characteristics of drinking water supply of population].

Science.gov (United States)

Nechaev, V S; Saurina, O S

2016-01-01

The article considers characteristics of organization ofprimary medical sanitary care on territory with carcinogenic risks related to drinking water supply as exemplified by the Orlovskaia oblast. The importance of registration by local health authorities the sources of permanent chemical pollution of drinking water. The analysis of the State program of the Orlovskaia oblast “The development of health care in the Orlovskaia oblast in 2013-2020". The necessity of additional inclusion of issue related to healthy drinking water supply of population to prevent development of malignant neoplasms and prevalence of oncologic morbidity on oblast territory.

Project of law relative to the sanitary consequences of French nuclear weapons tests

International Nuclear Information System (INIS)

2009-05-01

In order to make easy the indemnifications and to include the persons having participate to nuclear weapons tests (Sahara and French Polynesia) and populations leaving in the concerned areas, the project of law relative to the repair of sanitary consequences of nuclear weapons tests proposes to create a right to integral repair of prejudices for the persons suffering of a radioinduced disease coming from these tests. The American example and the British example are given for comparison. The modalities of financing are detailed as well as the social economic and administrative impacts. (N.C.)

Sanitary landfill groundwater quality assessment plan Savannah River Site

Energy Technology Data Exchange (ETDEWEB)

Wells, D.G.; Cook, J.W.

1990-06-01

This assessment monitoring plan has been prepared in accordance with the guidance provided by the SCDHEC in a letter dated December 7, 1989 from Pearson to Wright and a letter dated October 9, 1989 from Keisler to Lindler. The letters are included a Appendix A, for informational purposes. Included in the plan are all of the monitoring data from the landfill monitoring wells for 1989, and a description of the present monitoring well network. The plan proposes thirty-two new wells and an extensive coring project that includes eleven soil borings. Locations of the proposed wells attempt to follow the SCDHEC guidelines and are downgradient, sidegradient and in the heart of suspected contaminant plumes. Also included in the plan is the current Savannah River Site Sampling and Analysis Plan and the well construction records for all of the existing monitoring wells around the sanitary landfill.

Estimation of Parameters and Flow Characteristics for the Design of Sanitary Sewers in Malaysia

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Kamran Ansari

2013-01-01

Full Text Available Determination of the flow characteristics is very important for the design of sanitary sewers in any area. In the present study these are determined in the running sewers for the two parameters; per capita flow contribution and the peak flow factor. ISCO area - velocity flow meter model 4250 is used for this purpose. The flow meter, before being used in the running sewers, is calibrated first in the Hydraulics and Hydrology Laboratory of the UTM (Universiti Teknologi Malaysia. During the study the flow meter is installed inside the manhole in 10 different phases in the months of June, August, September, and October 2005 to monitor the sewage flow running in it. Continuous data is recorded in the flow meter during the process and the recorded time varies between 47 hours 25 minutes and 128 hours 35 minutes. The rainfall data is also collected during the same time using an automatic rain gauge which recorded rainfall at every five minutes of interval. Both the parameters thus calculated are then compared with the Malaysian Standard for sewer design i.e. MS 1228:1991. The results show that higher values of these parameters are being used in the design of sanitary sewers and extensive study needs to be carried out to review these values for future use

Physical and sanitary quality of soybean seeds produced in the state of Santa Catarina

Directory of Open Access Journals (Sweden)

Volmir Frandoloso

2015-08-01

Full Text Available Soybean production accounts for approximately 43% of Brazil’s grain produce, worth over 81 million tons for the 2012- 2013 cropping season. The use of quality seeds is essential for a high-yield agriculture, since low quality seeds compromise plant standing and increase production costs due to reseeding. This will in turn reduce yields through delays on the establishment dates. Seed quality is affected by relative humidity levels, mechanical damage during harvest and processing, thermal damage during the drying process and the environmental conditions during storage that could foster insect and fungi damage. The climate in the state of Santa Catarina favors soybean seed production, and seed companies in general work with high levels of technology. This work is aimed at characterizing the physical and sanitary quality of soybean seeds produced by different companies at different locations throughout the state of Santa Catarina (municipalities of Xanxerê, Abelardo Luz, Campos Novos and Canoinhas. The physical purity, moisture level, sanitary quality and mechanical damage of soybean seeds were studied at three stages, i.e. reception at the seed processing unit, at the end of the processing process and before being marketed. Results pointed out at mechanical injury and cultivar mixture as the main issues concerning seed lot quality, and that soybean seeds produced in Santa Catarina show pathogen contamination, which emphasizes the need for seed treatment.

Evaluation of the sanitary and environmental risks resulting from the Erika sinking and from the coasts cleaning operations

International Nuclear Information System (INIS)

Cicolella, A.

2000-03-01

This synthesis presents the methodology of the risks evaluation and of the INERIS report, the products analysis, general data on polycyclic aromatic hydrocarbons, the exposure evaluation, the sanitary impact evaluation for the polycyclic organic hydrocarbons and volatile organic compounds and the evaluation of ecological risks. (A.L.B.)

[Identification and detection of trag: a new infection-related gene expressed in vivo from isolates of Streptococcus suis].

Science.gov (United States)

Zhu, Haodan; Gu, Hongwei; Lu, Chengping

2008-12-01

The trag (transfer gene G) was one of the novel infection-related factors identified by in vivo-induced antigen technology (IVIAT) from Streptococcus suis type 2 expression libraries with swine convalesecent sera in our former research. We detected the distribution of trag in different Streptococcus suis isolates and identify the differential expression of the new infection-related factor between in vivo and in vitro condition. According to the sequence of trag of North American strain 89/1591, a pair of primers were designed to detect the distribution of trag in total 43 SS isolates. Another pair of primers were designed to amplify the ORF of trag of 5 SS representive strains (ZY05719, HA9801, 98012, SH040805, SH040917). Partial gene of trag was cloned and inserted into expression vector pET28a(+), and induced by IPTG to express recombinant TRAG. The recombinant protein was probed with swine convalescent sera and immune sera respectively. The trag was detected in the most of SS2 isolates (30/32), in SS9 isolates (4/6), and 1 isolate of SS7, while it was not found in SS2 European strain ATCC43765, avirulent strain SS2 T15, 1 isolates of SS1, 1 isolates of SS1/2 and 2 isolates of group C streptococcal strains from pigs. Comparisons between the sequences of TRAG of 5 isolates with that of SS isolates, showed a high homology (>97%) with North American strain 89/1589 and China strains 98HAH33, 05ZYH33. The immunoreactivity was only presented with convalescent sera. The trag was detected from virulent SS isolates but not from avirulent strain, which suggested that this gene may be related to the pathogenicity of SS. The special reactivity was only present with convalescent sera, and it indicated that TRAG might play a role during SS2 invasive course.

Identification and characterization of a novel protective antigen, Enolase of Streptococcus suis serotype 2.

Science.gov (United States)

Zhang, Anding; Chen, Bo; Mu, Xiaofeng; Li, Ran; Zheng, Pei; Zhao, Yaxin; Chen, Huanchun; Jin, Meilin

2009-02-25

Streptococcus suis serotype 2 (SS2) is a porcine and human pathogen with adhesive and invasive properties. The absence of suitable vaccine or virulent marker can be the bottleneck to control SS2 infection. In the present study, a novel immunogenic Enolase identified in the previous study was inducibly overexpressed in Escherichia coli, and the purified recombinant protein could elicit a significant humoral antibody response and confer efficient immunity against challenge with lethal dose of SS2 or SS7 infection in mouse model. The roles Enolase plays in pathogenicity of SS2 were also explored as reasons for which Enolase could be a protective antigen. The Enolase was an in vivo-induced antigen confirmed by the real-time PCR and could adhere to the Hep-2 cells by the indirect immunofluorescent assay and the inhibition assay. These suggested that Enolase could play important roles in pathogenicity and may serve as a novel vaccine candidate against SS2 infection.

Update summary of the 1991 discharge of enriched uranium to the sanitary sewer (DEUSS) report

International Nuclear Information System (INIS)

1995-04-01

Since the 1991 Y-12 Plant report, a flow study of the Y-12 Plant sanitary sewer collection system has been completed by the Y-12 Plant staff (Hanzelka and Maguire, 1993). Additional data has been obtained by the Y-12 Plant and the City of Oak Ridge (COR, 1994). COR developed limits on radionuclide concentrations in sludges used for land application (Stetar, 1993). Martin Marietta Energy Systems has provided recommendations to the Department of Energy (DOE) regarding the impacts of sludge land farming operations on the ORR (Frye, 1992). The DOE Office of Nuclear Safety (USDOE, 1993) completed an audit of activities related to radiological contamination of the COR sewer system due to DOE operations. In addition COR is currently developing limits on radionuclide releases for all industrial customers to be applied through the permitting process. In 1994, a new sanitary sewer monitoring station was installed and began operation at the Y-12 Plant to determine releases specifically from the Y-12 Plant. Previously, estimates were based on mass balance calculations using data from the City Monitoring Station which monitors Union Valley and Y-12 Plant releases. The purpose of this report is to update the 1991 Y-12 Plant study taking into account current data and information

In vitro sensitivity of Hungarian Actinobaculum suis strains to selected antimicrobials.

Science.gov (United States)

Biksi, I; Major, Andrea; Fodor, L; Szenci, O; Vetési, F

2003-01-01

In vitro antimicrobial sensitivity of 12 Hungarian isolates and the type strain ATCC 33144 of Actinobaculum suis to different antimicrobial compounds was determined both by the agar dilution and by the disc diffusion method. By agar dilution, MIC50 values in the range of 0.05-3.125 micrograms/ml were determined for penicillin, ampicillin, ceftiofur, doxycycline, tylosin, pleuromutilins, chloramphenicol, florfenicol, enrofloxacin and lincomycin. The MIC50 value of oxytetracycline and spectinomycin was 6.25 and 12.5 micrograms/ml, respectively. For ofloxacin, flumequine, neomycin, streptomycin, gentamicin, nalidixic acid, nitrofurantoin and sulphamethoxazole + trimethoprim MIC50 values were in the range of 25-100 micrograms/ml. With the disc diffusion method, all strains were sensitive to penicillin, cephalosporins examined, chloramphenicol and florfenicol, tetracyclines examined, pleuromutilins, lincomycin and tylosin. Variable sensitivity was observed for fluoroquinolones (flumequine, enrofloxacin, ofloxacin), most of the strains were susceptible to marbofloxacin. Almost all strains were resistant to aminoglycosides but most of them were sensitive to spectinomycin. A strong correlation was determined for disc diffusion and MIC results (Spearman's rho 0.789, p < 0001). MIC values of the type strain and MIC50 values of other tested strains did not differ significantly. Few strains showed a partially distinct resistance pattern for erythromycin, lincomycin and ampicillin in both methods.

The effect of sanitary landfill leachate aging on the biological treatment and assessment of photoelectrooxidation as a pre-treatment process

Energy Technology Data Exchange (ETDEWEB)

Müller, Gabriel Timm [Universidade Estadual do Rio Grande do Sul (UERGS), R. Gal. João Manoel, 50, CEP 90010-030 Porto Alegre, RS (Brazil); Giacobbo, Alexandre [Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Setor 4, Prédio 74, CEP 91501-970 Porto Alegre, RS (Brazil); Santos Chiaramonte, Edson Abel dos [Universidade Estadual do Rio Grande do Sul (UERGS), R. Gal. João Manoel, 50, CEP 90010-030 Porto Alegre, RS (Brazil); Rodrigues, Marco Antônio Siqueira [Universidade FEEVALE, ICET, RS 239, 2755, CEP 93352-000 Novo Hamburgo, RS (Brazil); Meneguzzi, Alvaro [Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Setor 4, Prédio 74, CEP 91501-970 Porto Alegre, RS (Brazil); Bernardes, Andréa Moura, E-mail: amb@ufrgs.br [Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Setor 4, Prédio 74, CEP 91501-970 Porto Alegre, RS (Brazil)

2015-02-15

Highlights: • Photoelectrooxidation (PEO) emerges as a new technology for leachate treatment. • Aging of sanitary landfills acts on leachate composition and biodegradability. • PEO is applied as leachate pretreatment before the biological processes. • PEO produced significant changes in the leachate matrix, easing biological process. - Abstract: The sanitary landfill leachate is a dark liquid, of highly variable composition, with recalcitrant features that hamper conventional biological treatment. The physical–chemical characteristics of the leachate along the landfill aging, as well as their effects on the efficiency of the conventional treatment, were evaluated at this paper. The feasibility of photoelectrooxidation process as an alternative technique for treatment of landfill leachates was also determined. Photoelectrooxidation experiments were conducted in a bench-scale reactor. Analysis of the raw leachate revealed many critical parameters demonstrating that the recalcitrance of leachate tends to increase with time, directly influencing the decline in efficiency of the conventional treatment currently employed. The effects of current density and lamp power were investigated. Using a 400 W power lamp and a current density of 31.5 mA cm{sup −2}, 53% and 61% efficiency for the removal of ammoniacal nitrogen and chemical oxygen demand were respectively achieved by applying photoelectrooxidation process. With the removal of these pollutants, downstream biological treatment should be improved. These results demonstrate that photoelectrooxidation is a feasible technique for the treatment of sanitary landfill leachate, even considering this effluent’s high resistance to treatment.

The effect of sanitary landfill leachate aging on the biological treatment and assessment of photoelectrooxidation as a pre-treatment process

International Nuclear Information System (INIS)

Müller, Gabriel Timm; Giacobbo, Alexandre; Santos Chiaramonte, Edson Abel dos; Rodrigues, Marco Antônio Siqueira; Meneguzzi, Alvaro; Bernardes, Andréa Moura

2015-01-01

Highlights: • Photoelectrooxidation (PEO) emerges as a new technology for leachate treatment. • Aging of sanitary landfills acts on leachate composition and biodegradability. • PEO is applied as leachate pretreatment before the biological processes. • PEO produced significant changes in the leachate matrix, easing biological process. - Abstract: The sanitary landfill leachate is a dark liquid, of highly variable composition, with recalcitrant features that hamper conventional biological treatment. The physical–chemical characteristics of the leachate along the landfill aging, as well as their effects on the efficiency of the conventional treatment, were evaluated at this paper. The feasibility of photoelectrooxidation process as an alternative technique for treatment of landfill leachates was also determined. Photoelectrooxidation experiments were conducted in a bench-scale reactor. Analysis of the raw leachate revealed many critical parameters demonstrating that the recalcitrance of leachate tends to increase with time, directly influencing the decline in efficiency of the conventional treatment currently employed. The effects of current density and lamp power were investigated. Using a 400 W power lamp and a current density of 31.5 mA cm −2 , 53% and 61% efficiency for the removal of ammoniacal nitrogen and chemical oxygen demand were respectively achieved by applying photoelectrooxidation process. With the removal of these pollutants, downstream biological treatment should be improved. These results demonstrate that photoelectrooxidation is a feasible technique for the treatment of sanitary landfill leachate, even considering this effluent’s high resistance to treatment

On the development of the system of risk-based supervision in the field of sanitary and epidemiological welfare of the population and consumer protection

Directory of Open Access Journals (Sweden)

A.Y. Popova

2015-12-01

Full Text Available In order to fulfill the legal requirements for the transition to the new risk-based model of the state control (supervision in the Federal Service for Consumer Rights Protection and Human Welfare, the methodical approaches to the classification of economic entities and activities of potential risk of injury have been developed. The risk assessment takes into account the frequency of violations of sanitary legislation, the severity of the consequences of these violations for the health and extent of negative effects, which is estimated in terms of population under the influence of the economic entity. Algorithms and methods for calculating the exposed population: workers, consumers, including consumer food products, non-food products and services; residents of settlements under the negative impact of air pollution, water and soil, have been developed. Regional and federal registries of objects for sanitary and epidemiological supervision are formed. Testing of the risk-based approaches in the regions allowed excluding the low risk harm objects when forming the audit plans for 2016, due to what the number of planned inspections all over country was reduces by more than 20 %. The basic directions of further improvement of the risk-based model of sanitary and epidemiological surveillance: its extension to the system of technical regulation and consumer protection; scientific substantiation of its volume, content and laboratory support of the scheduled inspections of objects belonging to different classes of health risk; improving procedures and methods for evaluating the effectiveness and efficiency of risk-based supervisory activities; creation of an effective system of risk communication between the sanitary and epidemiological surveillance, economic entities, public organizations and associations and civil society.

SANITARY-HYGIENIC ASPECTS IN COMMERCIALIZATION IN FISH MARKET IGARAPÉ DAS MULHERES, MACAPÁ-AP

Directory of Open Access Journals (Sweden)

Antonio Carlos Souza Silva Junior

2016-12-01

Full Text Available Being highly perishable, the fish requires special care from capture to arrival at the consumer's home. However, after the capture, the microbiota is altered at all stages. This study aimed to assess the sanitary conditions of marketing of fish in the Fish Friday, Macapá-AP, in order to identify critical points of the process. We used the direct observational method with the application of a checklist, based on the RDC nº216, items for building, equipment / instruments, utensils and hygiene, clothing, hygienic habits, personal protective equipment and quality raw material. The fair in question was rated in Group 2, failing to meet the requirements of industry topics Organization (marketing space extremely limited, national manager indefinite absence of good practice manual, along with the standard operating procedures; hygiene observation site (fish is exposed without ice, ice used only for storage, water use without treatment, and cleaned the handler (clothing unsatisfactory, tobacco consumption during marketing, simultaneous manipulation of money and food, no clean hands when handling food thus does not meet the provisions of the legislation. Such unconformities compromise the quality of products and endangers the health of the consumer, demonstrating that only the drafting of laws is not sufficient to ensure food safety without intense scrutiny in conjunction with the drafting resolutions appropriate to the reality of each region and training programs aimed at promoting recycling and the production of safe food hygiene and sanitary conditions in the question that reality cannot be changed. Keywords: marketing of fish; conditions of merchantability; market.

Present and prospective developments in health science and sanitary supervision relative to use of atomic energy in national economy

International Nuclear Information System (INIS)

Lyarskij, P.P.; Mojsejtsev, P.I.; Vasil'ev, G.

1977-01-01

The discoveries and inventions made over the last two decades have substantially enlarged the scope of contacts between man and sources of ionizing radiation. Scientific developments raising energy yield from nuclear processes have been reflected in a growing nuclear power industry as well as expanding use of radioactive substances or radiation sources in various areas of human endeavor, and have become a potent factor in technological progress, mechanization and automation. No such tempestuous expansion of applications of nuclear physics achievements would have been possible without the concurrent development and further synchronous advances in relevant fields of health science, with research achievements promptly introduced into sanitary supervision practice. The paper summarizes major results of research in radiation hygiene; outlines the impact of their application to sanitary supervision practice in principal operational sectors; identifies and motivates further lines of injury as well as prospects for developing up-to-date supervision schemes and for reasonable unification of measurement and monitoring techniques. (author)

Creosote released from railway-ties recycled and the sanitary risks.

Science.gov (United States)

Zicari, Giuseppe; Allegro, Giuseppe; Russo, Domenico; Rivetti, Daniela; Soardo, Vincenzo; Cerrato, Elena

2013-01-01

Through the history of railways, wooden crossties impregnated with potentially hazardous creosote tar, have been used for years. There are six major classes of compounds in the creosote: aromatic hydrocarbons; tar acids/phenolics; tar bases/nitrogen-containing heterocycles; aromatic amines; sulfur-containing heterocycles; and oxygen-containing heterocycles. The creosote molecules applied in railway crossties can be released in the environment and they can bioaccumulate in animals and vegetables. Some constituents (benzo(a)pyrene and phenolics like benzene) are considered as being carcinogenic which renders the entire complex of creosote to be classified as potentially carcinogenic. After several decades of use the railway-ties are been recycled for varies uses like fences, stakes for agriculture and fruit production or bank protection. In this paper are examined some environmental and sanitary risks from wood impregnated with creosote reported in the literature.

Remaining Sites Verification Package for the 100-F-26:10, 1607-F3 Sanitary Sewer Pipelines (182-F, 183-F, and 151-F Sanitary Sewer Lines). Attachment to Waste Site Reclassification Form 2007-028

International Nuclear Information System (INIS)

Dittmer, L.M.

2007-01-01

The 100-F-26:10 waste site includes sanitary sewer lines that serviced the former 182-F, 183-F, and 151-F Buildings. In accordance with this evaluation, the verification sampling results support a reclassification of this site to Interim Closed Out. The results of verification sampling show that residual contaminant concentrations do not preclude any future uses and allow for unrestricted use of shallow zone soils. The results also demonstrate that residual contaminant concentrations are protective of groundwater and the Columbia River

Physical characterization and sanitary conditions of cheese type cabacinha market places in three municipalities of the Jequitinhonha Valley, MG, Brazil

Directory of Open Access Journals (Sweden)

Adair da Silva Santos Filho

2017-09-01

Full Text Available Cheese type cabacinha from Vale do Jequitinhonha is obtained by curd heating in a similar process made to Mozzarella, but the raw milk is unpasteurized and the final product is stored unpackaged and at room temperature. This milk derivative may contribute to the increase the income of the local population, especially the residents of the edges of roads, due to the flow of vehicles and possible buyers. The purpose of this work was to identify the physical and sanitary conditions of cheese type cabacinha market places present in three municipalities of the Vale do Jequitinhonha, MG, Brazil. Previously the investigation was detected the number of marketplaces that sell these cheese in the Medina, Cachoeira de Pajeú and Pedra Azul, municipalities of the state of Minas Gerais, Brazil, located on the surrounding area of the highways BR 251 and BR 116. Afterward as developed a physical and sanitary checklist and it were filled in loco. It was observed that most market places were not provided of masonry walls, piped water or toilet and sink. It is also common to the presence of possible contamination vectors in surroundings, such as dogs and insects. It is verified that most of these cheese is exposed in pairs held by string and unpackaged. This study demonstrated the precariousness of the physical structures and consequently the lack of adequate sanitary conditions in the cheese type cabacinha market places. Because it is an artisanal product from family farming, which generates income and employment in the field deserve more attention of competent state and municipal authorities.

Estimating the Prevalence of Potential Enteropathogenic Escherichia coli and Intimin Gene Diversity in a Human Community by Monitoring Sanitary Sewage

Science.gov (United States)

Yang, Kun; Pagaling, Eulyn

2014-01-01

Presently, the understanding of bacterial enteric diseases in the community and their virulence factors relies almost exclusively on clinical disease reporting and examination of clinical pathogen isolates. This study aimed to investigate the feasibility of an alternative approach that monitors potential enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) prevalence and intimin gene (eae) diversity in a community by directly quantifying and characterizing target virulence genes in the sanitary sewage. The quantitative PCR (qPCR) quantification of the eae, stx1, and stx2 genes in sanitary sewage samples collected over a 13-month period detected eae in all 13 monthly sewage samples at significantly higher abundance (93 to 7,240 calibrator cell equivalents [CCE]/100 ml) than stx1 and stx2, which were detected sporadically. The prevalence level of potential EPEC in the sanitary sewage was estimated by calculating the ratio of eae to uidA, which averaged 1.0% (σ = 0.4%) over the 13-month period. Cloning and sequencing of the eae gene directly from the sewage samples covered the majority of the eae diversity in the sewage and detected 17 unique eae alleles belonging to 14 subtypes. Among them, eae-β2 was identified to be the most prevalent subtype in the sewage, with the highest detection frequency in the clone libraries (41.2%) and within the different sampling months (85.7%). Additionally, sewage and environmental E. coli isolates were also obtained and used to determine the detection frequencies of the virulence genes as well as eae genetic diversity for comparison. PMID:24141131

[The activity of the State Sanitary Inspectorate after implementation of the act of January, 23 2009 "Amending certain acts regarding reorganization and redistribution of competences of the public administration at the provincial level"].

Science.gov (United States)

Rudaś, Dariusz; Skórczewski, Krzysztof

2011-01-01

The restructuring of the State Sanitary Inspectorate was determined by the need to adjust the existing system to the new structure- and task-oriented standards introduced by the administrative reforms at the provincial level. This brought about a lot of changes in the way State County Sanitary Inspectors operate. For first few months after the enforcement of the Act of January 23.2009 amending certain acts regarding reorganization and redistribution of competences of the public administration at the provincial level (Journal of Laws, No. 92, item. 753, 2009) brought about a lot of ambiguities and expectations. Such unresolved issues are typical for the initial or transition period after implementation of a new law. The approach to the operation of the State Sanitary Inspectorate at the county level depicted in the article lays the emphasis on the challenges in the legal and task-oriented domain that arose after coming into force of the aforementioned amendment. By way of conclusion, the authors of the article briefly summaries the practical outcomes of the transformations undergone by the State Sanitary Inspectorate at the county level.

ICT: interfacce tra persone e luoghi

Directory of Open Access Journals (Sweden)

Corinna Morandi

2013-04-01

Full Text Available Nonostante la diffusione del paradigma della smart city sia stata esponenziale, con l’effetto di confonderne il significato, il concetto di “città intelligente” indica comunque un tema di ricerca importante, in particolare quando venga esteso a contesti territoriali più ampi. In questo senso, la ricerca La smart region tra Torino e Milano. I servizi mobili come driver di innovazione territoriale in vista di Expo 2015 (Politecnico di Milano-Telecom Italia si propone di sperimentare l’uso delle ICT per un sistema integrato di servizi nel territorio tra Torino e Milano, anche in prospettiva di Expo 2015: una regione metropolitana valorizzata da un sistema di relazioni già attive e inserita nel contesto più ampio della city-region del Nord Italia, il cui sviluppo va legato ad una strategia di scala vasta, declinabile nella definizione di “smart city-region” o, più semplicemente, di “smart region”. In tale contesto, il ruolo delle università nei processi di sviluppo territoriale, il miglioramento dei servizi materiali e immateriali erogati dai campus, su cui la ricerca (in corso ha scelto di concentrarsi, può contribuire ad aumentare la sostenibilità e competitività del territorio. Una prima sperimentazione è stata condotta sull’area di Città Studi a Milano, testando una metodologia utile a comprenderne le esigenze e individuando soluzioni e servizi funzionanti su  terminali di comunicazione mobile, interfaccia tra persone e luoghi nel campus e nel contesto urbano circostante.

“Sui Generis” Journalism? Visibility, Identities and Journalistic Practices in a 1990s Brazilian Gay Magazine

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Ricardo Augusto de Saboia Feitosa

2018-04-01

Full Text Available This article examines the Sui Generis magazine (1995-2000, one of the most relevant gay press magazines in Brazil. We study the importance of this publication and the field of journalism in terms of producing and reproducing representations and processes of identities and relations of gender and sexuality. The methodology involves a discursive reading of editorials, cover stories and reader letters published between January 1995 and March 2000, as well as a restructuring of journalistic work and daily practices in newsrooms through interviews with reporters, columnists and editors. We reached the conclusion that choosing a policy of visibility based on outing and concepts of gay "identities" and "communities" allowed the magazine to create specific and more valued ways of what homosexuality is, which leads to a critical reflection on what these policies have achieved, and what their limits and tensions are. O artigo propõe uma investigação da revista Sui Generis (1995-2000, título do segmento especializado designado como “imprensa gay” brasileira. Busca-se compreender a relevância da publicação e deste campo jornalístico como instâncias historicamente produtoras e reprodutoras tanto de representações como dos processos de agenciamento de identidades e relações de gênero e sexualidade. Adota-se como metodologia a leitura discursiva de editoriais, reportagens de capa e cartas dos leitores publicados entre janeiro de 1995 e março de 2000; e a reconstituição, por meio de entrevistas com repórteres, colunistas e editores, do fazer jornalístico e das práticas cotidianas da redação. A análise permite constatar que, ao eleger como estratégia uma política de visibilidade calcada no outing e na elaboração de noções de “identidade” e “comunidade” gays, forjam-se modos específicos e mais valorizados do que seria a homossexualidade, exigindo uma reflexão crítica das conquistas dessas políticas, dos seus limites e

Characteristic dosimetric as of the material thermoluminescent LIF:Mg, Cu, and P for their use in sanitary applications

International Nuclear Information System (INIS)

Ginjaume, M.; Ortega, X.; Duch, M.A.; Sanchez-Reyes, A.

1998-01-01

This work presents the characteristics dosimetric of the LiF:Mg, Cu, P obtained for its use in the sanitary field, fundamentally in radiotherapy. The analyzed parameters are sensitivity, reproducibility of the measures, limit inferior of detection, answer energy, linearity of the answer in function of the received dose and the influence of the temperature in the material

Radioisotope devices and sanitary control over its application in the units and institutions of Army and Navy

International Nuclear Information System (INIS)

Velichkin, E.V.; Rymarchuk, A.A.; Shishkanov, A.P.

1991-01-01

Sanitary control over the use of radioisotope devices (RID) is considered. To avoid losses of radioactive sources, an attention is paid to the order of RID account, their storage and issue, writing off unfit devices and sources and their burial. The role of military medical personnel grows. They must provide efficiency of safety of radioactive substance use in the units of Army and Navy

New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide

Science.gov (United States)

Casabuono, Adriana C.; Czibener, Cecilia; Del Giudice, Mariela G.; Valguarnera, Ezequiel; Ugalde, Juan E.; Couto, Alicia S.

2017-12-01

Brucellaceae are Gram-negative bacteria that cause brucellosis, one of the most distributed worldwide zoonosis, transmitted to humans by contact with either infected animals or their products. The lipopolysaccharide exposed on the cell surface has been intensively studied and is considered a major virulence factor of Brucella. In the last years, structural studies allowed the determination of new structures in the core oligosaccharide and the O-antigen of this lipopolysaccharide. In this work, we have reinvestigated the lipid A structure isolated from B. suis and B. abortus lipopolysaccharides. A detailed study by MALDI-TOF mass spectrometry in the positive and negative ion modes of the lipid A moieties purified from both species was performed. Interestingly, a new feature was detected: the presence of a pyrophosphorylethanolamine residue substituting the backbone. LID-MS/MS analysis of some of the detected ions allowed assurance that the Lipid A structure composed by the diGlcN3N disaccharide, mainly hexa-acylated and penta-acylated, bearing one phosphate and one pyrophosphorylethanolamine residue. [Figure not available: see fulltext.

Hygienic-sanitary working practices and implementation of a Hazard Analysis and Critical Control Point (HACCP plan in lobster processing industries

Directory of Open Access Journals (Sweden)

Cristina Farias da Fonseca

2013-03-01

Full Text Available This study aimed to verify the hygienic-sanitary working practices and to create and implement a Hazard Analysis Critical Control Point (HACCP in two lobster processing industries in Pernambuco State, Brazil. The industries studied process frozen whole lobsters, frozen whole cooked lobsters, and frozen lobster tails for exportation. The application of the hygienic-sanitary checklist in the industries analyzed achieved conformity rates over 96% to the aspects evaluated. The use of the Hazard Analysis Critical Control Point (HACCP plan resulted in the detection of two critical control points (CCPs including the receiving and classification steps in the processing of frozen lobster and frozen lobster tails, and an additional critical control point (CCP was detected during the cooking step of processing of the whole frozen cooked lobster. The proper implementation of the Hazard Analysis Critical Control Point (HACCP plan in the lobster processing industries studied proved to be the safest and most cost-effective method to monitor each critical control point (CCP hazards.

Evaluation of sanitary consequences of Chernobyl accident in France: epidemiological monitoring device, state of knowledge, evaluation of risks and perspectives

International Nuclear Information System (INIS)

Verger, P.; Champion, D.; Gourmelon, P.; Hubert, Ph.; Joly, J.; Renaud, Ph.; Tirmarche, M.; Vidal, M.; Cherie-Challine, L.; Boutou, O.; Isnard, H.; Jouan, M.; Pirard, Ph.

2000-12-01

The objectives of this document are firstly, to present the situation of knowledge both on the sanitary consequences of the Chernobyl accident and on the risk factors of thyroid cancers, these ones constituting one of the most principal consequences observed in Belarus, in Ukraine and Russia; secondly, the give the principal system contributing to the epidemiological surveillance of effects coming from a exposure to ionizing radiations, in France and to give the knowledge on incidence and mortality of thyroid cancer in France; thirdly, to discuss the pertinence and the feasibility of epidemiological approaches that could be considered to answer questions that the public and authorities ask relatively to the sanitary consequences of Chernobyl accident in France; fourthly to male a calculation of thyroid cancer risk in relation with Chernobyl fallout in France from works and studies made from 1986 on the consequences of this disaster in terms of radioecology and dosimetry at the national level. Besides, the improvement of thyroid cancer surveillance is also tackled. (N.C.)

Sanitary and phytosanitary measures and food safety: challenges and opportunities for developing countries.

Science.gov (United States)

Siméon, M

2006-08-01

Because of fast-growing demand, export markets can absorb high value added products and offer high returns; for many developing countries export market development is thus a key requirement for rural income generation and rural growth. Although developing countries face increasingly strict sanitary and phytosanitary standards in their export markets, they can maintain and improve market access--and improve domestic food safety and agricultural productivity--by adopting a strategic approach to food safety, agricultural health and trade. High-income countries should increase development flows to help developing countries build the capacity to plan and execute the necessary strategies. The first proposal in this paper is to make two existing sets of guidelines widely available to interested parties, in particular through the World Bank and the World Organisation for Animal Health (OIE). The first covers the broad process of problem assessment, strategy development and action plan formulation; the second set deals with institutional analysis and training of staff of the official sanitary control services. The second proposal is that interested countries and donors should speed up the ongoing development of guidelines, computer software tools and training material to help countries quantify the importance and impact of food safety issues. The focus here is on a 'multipurpose agricultural data analysis and modelization system'. The third proposal is to carry out a case study to help demonstrate that a number of animal health issues related to food safety should be treated as relating to 'global public goods' and thus require intervention on a global scale. Possible candidates are foot and mouth disease and highly pathogenic avian influenza.

Realization of sanitary requirements concerning standardization of rare radioactive gas effluents at NPPs

International Nuclear Information System (INIS)

Doroshenko, G.G.; Panchenko, S.V.; Chudajkin, O.G.

1987-01-01

The paper is aimed at determination of ways for practical realization of main sanitary requirements concerning environment and population protection under NPP operation. The idea of the requirements is reduced to not increasing permissible limits for radiation doses, minimum irradiation of population and decrease of unjustified irradiation of personnel. The given problem may be fully solved only in case of studying real chacteristics of operating reactors, taking into account statistic nature of effluents of inert radioactive gases (IRG). Methods of developing a system of working and reference standards of IRG effluents at NPPs are suggested. The considered approach may be realized in practice as the All-Union state on branch standards

A qualitative understanding of the effects of reusable sanitary pads and puberty education: implications for future research and practice.

Science.gov (United States)

Hennegan, Julie; Dolan, Catherine; Steinfield, Laurel; Montgomery, Paul

2017-06-27

The management of menstruation has come to the fore as a barrier to girls' education attainment in low income contexts. Interventions have been proposed and piloted, but the emerging nature of the field means limited evidence is available to understand their pathways of effect. This study describes and compares schoolgirls' experiences of menstruation in rural Uganda at the conclusion of a controlled trial of puberty education and sanitary pad provision to elucidate pathways of effect in the interventions. Semi-structured interviews were undertaken with schoolgirls who participated in the Menstruation and the Cycle of Poverty trial concurrent with the final set of quantitative surveys. A framework approach and cross-case analysis were employed to describe and compare the experiences of 27 menstruating girls across the four intervention conditions; education (n = 8), reusable sanitary pads (n = 8), education with reusable sanitary pads (n = 6), and control (n = 5). Themes included: menstrual hygiene, soiling, irritation and infection, physical experience, knowledge of menstruation, psychological, social and cultural factors, and support from others. Those receiving reusable pads experienced improvements in comfort and reliability. This translated into reduced fears around garment soiling and related school absenteeism. Other menstrual hygiene challenges of washing, drying and privacy remained prominent. Puberty education improved girls' confidence to discuss menstruation and prompted additional support from teachers and peers. Findings have important implications for the development and evaluation of future interventions. Results suggest the provision of menstrual absorbents addresses one core barrier to menstrual health, but that interventions addressing broader needs such as privacy may improve effectiveness. Puberty education sessions should increase attention to body awareness and include strategies to address a wider range of practical menstrual challenges

Indagine sulle conoscenze dei modelli di prevenzione delle epatiti A e B nell'ambito di inchieste epidemiologiche condotte per tali patologie

Directory of Open Access Journals (Sweden)

P. Giametta

2003-05-01

Full Text Available

Obiettivi: rilevare le conoscenze sull’eziopatogenesi, sui modelli preventivi comportamentali e sulle offerte di vaccinoprofilassi delle Epatiti A e B.

Materiali e Metodi: l’indagine è stata effettuata su pazienti affetti da Epatite A e B integrando la scheda di inchiesta epidemiologica con un apposito questionario strutturato in tre sezioni. Nella prima sezione del questionario è stata valutata la conoscenza sull’eziopatogenesi delle epatiti; nella seconda sezione è stato saggiato il grado di conoscenza sui modelli comportamentali e sulla pratica dell’immunoprofilassi; nella terza è stato indagato nello specifico il probabile meccanismo di trasmissione con riferimento anche alla mancata adozione di eventuali pratiche di prevenzione possibili ed adottabili.

Il questionario è stato somministrato preliminarmente all’inchiesta epidemiologica.

Risultati: sono stati valutati complessivamente 85 questionari, nel periodo Gennaio 2002-Marzo 2003. I casi di Epatite A affrontati sono stati 19, mentre i casi di Epatite B sono stati 66. Una percentuale elevata di soggetti (89% era a conoscenza dell’eziologia virale e delle principali modalità di trasmissione. Apprezzabile la percentuale di soggetti (78% che non ha attribuito all’Epatite A una
trasmissione di tipo sessuale. La conoscenza dell’immunoprofilassi e delle offerte vaccinali condotte
dai servizi territoriali è risultata essere piuttosto bassa (34%. Per i casi di Epatite B, la causa più frequentemente indicata quale elemento determinante il contagio è stata individuata nei rapporti occasionali non protetti (anche a rischio. Per i casi di Epatite A, è da rilevare la superficialità al consumo di alimenti a rischio (frutti di mare o pesce
crudo soprattutto in occasione di viaggi all’estero.

Conclusioni: i dati suggeriscono l�

33 CFR 165.T09-1080 - Safety Zone and Regulated Navigation Area, Chicago Sanitary and Ship Canal, Romeoville, IL.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety Zone and Regulated Navigation Area, Chicago Sanitary and Ship Canal, Romeoville, IL. 165.T09-1080 Section 165.T09-1080 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED...

Combined heat pump for sanitary hot water and space heating with CO{sub 2} as refrigerant; Kombineret brugsvands- og rumvarmepumpe med CO{sub 2} som koelemiddel

Energy Technology Data Exchange (ETDEWEB)

Schoen Poulsen, C. [Teknologisk Institut (Denmark)

2006-05-19

This project report describes the implementation of the Danish project called 'Combined heat pump for sanitary hot water and space heating with CO{sub 2} as refrigerant'. In the course of the project, a combined heat pump has been developed for heating sanitary hot water and producing domestic space heating. The project shows that CO2 has excellent properties in systems where a high temperature is desired on the gas cooler side and that it is possible to combine the production of sanitary hot water with the production of domestic space heating. During the project, a number of system solutions have been analysed and at the end of the project a prototype was built. It was tested in the laboratory according to a current Dutch standard for heat pumps for sanitary hot water. The prototype was constructed without the space heat part which solely has been analysed according to calculations. The reason is that there currently are no applicable European standards for the testing of combined systems and as the total efficiency of the system mainly depends on the temperature out of the gas cooler it was decided not to spend resources on the construction of the combined system in the prototype version of the unit. Instead, a number of proposals have been submitted to how the system with a space heat section could be constructed. The main components used in the prototype (compressor, exchangers, valve, control and tank) are all partly commercially available and therefore focus has been on the system construction. During the project, a number of CFD calculations have been carried out on the gas cooler in the hot water tank and the results show how important it is that the gas cooler is designed and placed correctly. The laboratory tests carried out on the unit show that the COP of the heat pump plant in connection with sanitary hot water tapping (according to Dutch standard) is 1.4 1.5 which is not immediately satisfactory. But when it is considered that the unit is a

A Randomised, Double-blind, Placebo-controlled Trial of Trichuris suis ova in Active Crohn's Disease

DEFF Research Database (Denmark)

Schölmerich, Jürgen; Fellermann, Klaus; Seibold, Frank W

2017-01-01

Background and Aims: To investigate the efficacy and safety of three different dosages of embryonated, viable eggs of Trichuris suis [TSO] versus placebo for induction of remission in mildly-to-moderately active ileocolonic, uncomplicated Crohn's disease [CD]. Methods: Adults with active CD [n.......2%, and 47.2% of TSO 250, TSO 2500, and TSO 7500 patients, respectively, and in 42.9% of placebo recipients. TSO induced a dose-dependent immunological response. There was no response regarding laboratory markers of inflammation. Other secondary efficacy variables also showed no advantage of TSO over placebo...... for treatment of active CD. Administration of TSO did not result in any serious adverse drug reaction. Review of non-serious suspected adverse drug reactions following TSO did not reveal any safety concerns. Conclusions: Administration of 250-7500 TSO fortnightly over 12 weeks was safe and showed a dose...

Identifying suitable sanitary landfill locations in the state of Morelos, México, using a Geographic Information System

Science.gov (United States)

Marín, Luis E.; Torres, Vicente; Bolongaro, Andrea; Reyna, José A.; Pohle, O.; Hernández-Espriú, A.; Chavarría, Jerónimo; García-Barrios, R.; Tabla, Hugo Francisco Parra

GIS is a powerful tool that may help to better manage natural resources. In this paper, we present a GIS model developed for the state of Morelos as an aid to determine whether a potential site, Loma de Mejia, met the Mexican Federal Guidelines. The Mexican Government has established federal guidelines for sanitary landfill site selection (NOM-083-SERMARNAT-2003). These guidelines were translated into a water-based Geographic Information System and applied to the state of Morelos, Mexico. For these examples, we used the SIGAM® (Sistema de Información Geográfico del Agua en México; a water-based GIS for Mexico) which has at least 60 layers from the National Water Commission (CONAGUA), the national mapping agency (INEGI; Instituto Nacional de Estadística, Geografía e Informática), NASA, and academic institutions. Results show that a GIS is a powerful tool that may allow federal, state and municipal policy makers to conduct an initial regional site reconnaissance rapidly. Once potential sites are selected, further characterization must be carried out in order to determine if proposed locations are suitable or not for a sanitary landfill. Based on the SIGAM© software, the Loma de Mejia would not comply with the Mexican Federal Guidelines.

Environmental and sanitary evaluation of electro-nuclear sites: methodological research and application to prospective scenarios

International Nuclear Information System (INIS)

2004-12-01

In the framework of the radioactive wastes disposal of the law of 1991, an exchange forum constituted by ANDRA, CEA, COGEMA, EdF, Framatome-ANP and IRSN implemented an environmental and sanitary evaluation of the different methods of radioactive wastes management. This report presents the six studies scenarios, the proposed methodology, the application to the six scenarios and the analysis of the results which showed the efficiency of the different recycling options towards the electronuclear cycle impacts limitation, and a technical conclusion illustrated by improvement possibilities of the methodology. (A.L.B.)

[Expression, purification and protective antigen analysis of cell wall protein MRP of Streptococcus suis type 2].

Science.gov (United States)

Wang, Ping-ping; Pian, Ya-ya; Yuan, Yuan; Zheng, Yu-ling; Jiang, Yong-qiang; Xiong, Zheng-ying

2012-02-01

To amplify the mrp gene of Streptococcus suis type 2 05ZYH33, express it in E.coli BL21 in order to acquire high purity recombinant protein MRP, then evaluate the protective antigen of recombinant protein MRP. Using PCR technology to obtain the product of mrp gene of 05ZYH33, and then cloned it into the expression vector pET28a(+). The recombinant protein was purified by affinity chromatography, later immunized New Zealand rabbit to gain anti-serum, then test the anti-serum titer by ELISA. The opsonophagocytic killing test demonstrated the abilities of protective antigen of MRP. The truncated of MRP recombinant protein in E.coli BL21 expressed by inclusion bodies, and purified it in high purity. After immunoprotection, the survival condition of CD-1 was significantly elevated. The survival rate of wild-type strain 05ZYH33 in blood was apparently decreased after anti-serum opsonophagocyticed, but the mutant delta; MRP showed no differences. MRP represent an important protective antigen activity.

Possible pathogenic interplay between Chlamydia suis, Chlamydophila abortus and PCV-2 on a pig production farm.

Science.gov (United States)

Schautteet, K; Beeckman, D S A; Delava, P; Vanrompay, D

2010-03-13

A concurrent outbreak of chlamydial disease in boars, sows and gilts and postweaning multisystemic wasting syndrome (PMWS) in weaned piglets was investigated on a large pig production farm in Estonia. Chlamydia suis DNA was detected in conjunctival swabs from boars, sows and gilts, but also in the faeces of boars and sows. Chlamydophila abortus DNA was found in semen, and in conjunctival swabs from sows; DNA was demonstrated by microarrays. Serum samples from boars were examined using a Chlamydiaceae-specific recombinant ELISA. All 10 serum samples examined were positive (1:960 to 1:3840). Chlamydiosis was characterised by reproductive failure and conjunctivitis. Piglets were not examined for Chlamydiaceae, as eye problems were not observed. Piglets showed wasting, respiratory signs, diarrhoea, enlargement of lymph nodes and increased mortality (10 per cent). Porcine circovirus type 2 (PCV-2) was detected in the lymph nodes of piglets by immunohistochemistry, and PCV-2 antibodies were demonstrated in all 10 serum samples from sows examined using an immunoperoxidase monolayer assay.

[Sanitary and epidemiological well-being in children and adolescents: state-of-the-art and ways of solving the problem].

Science.gov (United States)

Onishchenko, G G

2007-01-01

In the children and adolescents of the Russian Federation, there is an increase in the incidence of a number of nosological entities, a significant prevalence of chronic diseases, and a reduction in health quality in children. According to the statistical data, in the past 5 years, the overall incidence has increased by 16% in children (aged 0-14 years) (171268 per 100,000 children in 2005) by 18% in adolescents. The incidence of diseases of blood and blood-forming organs, anemias, as well diseases of the respiratory, urogenital, and nervous systems, and congenital anomalies continues to increase. Along with socioeconomic causes, this is all due to the negative influence of environmental factors, dietary defects, and, in some cases, poor upbringing and teaching conditions, which do not comply with the hygienic standards and sanitary rules. Under the established conditions, the maintenance and improvement of children's health is the most important state task, the provision of their sanitary and epidemiological welfare being one of the major constituents in its accomplishment. In the past 3 years, the principal state sanitary inspector has issued 17 regulations on different aspects of the sanitary and epidemiological well-being of children and adolescents. The preparation of the draft "National Program for the Prevention of Adverse Health Influence of Environmental Factors for the Sake of the Future of Children and Adolescents for 2007-2010" has been currently completed. The Group of Eight Summit has noted that resolute opposition to threats of infectious diseases, the leading cause of death in the world, is vital to the global development and welfare of mankind. It has adopted the resolution that it is essential to make specific achievements in the strengthening international cooperation in the surveillance and monitoring of infectious diseases; to intensify researches; to second the efforts of appropriate international organizations, which are aimed at effectively

Behavior of radionuclides in sanitary landfills.

Science.gov (United States)

Chang, K C; Chian, E S; Pohland, F G; Cross, W H; Roland, L; Kahn, B

1984-01-01

his study was undertaken to evaluate the possibility of disposing low-level radioactive waste in sanitary landfills with leachate containment to prevent environmental releases. To meet this objective, two simulated landfills, each 200 l. in volume and containing 55 kg of municipal refuse, were operated in the laboratory with simulated rainfall additions for a 9-month period to observe the extent to which radio-cobalt, -cesium, -strontium and tritium were leached into the liquid phase. One of the units was operated with leachate recycle, the other as a single pass control. Liquid samples were analyzed weekly for 3H, 58Co, 85Sr and 134Cs tracers. Weekly analyses were also performed for approximately 30 parameters to define the degree of stabilization of the waste. Major parameters included BOD, COD, pH and concentrations of specific organics, metals and gases. Concentrations of stable cobalt, strontium and cesium were also measured periodically. Soluble radioactivity levels in both systems were reduced by factors of 50 for 58Co, 5 for 85Sr and 7 for 134Cs, taking radioactive decay and dilution into account. Some radionuclide removal from the liquid phase was associated with major chemical changes in the landfills that occurred within 80 days for the control system and within 130 days for the recycle unit. Observed acid, sulfide, and CO2 concentrations suggested mechanisms for removing some of the radionuclides from leachate. Detection of 3H in the off-gas indicated that less than 1% of tritiated waste became airborne. The waste in the leachate recycle unit was more completely stabilized than in the control unit.

[Evaluation of the presence of hygienic and sanitary indicator microorganisms in food served in public schools in Porto Alegre, Brazil].

Science.gov (United States)

de Oliveira, Ana Beatriz Almeida; Capalonga, Roberta; Silveira, Joice Trindade; Tondo, Eduardo Cesar; Cardoso, Marisa Ribeiro de Itapema

2013-04-01

The objective of this study was to evaluate the presence of hygienic and sanitary indicator microorganisms in samples of food served in public schools in Porto Alegre. All the food served in the meal of the session visited was analyzed for Escherichia coli, coagulase-positive Staphylococcus, Salmonella sp. and Shigella sp. Of the total of 196 food products analyzed in 120 schools, 4 contained and Escherichia coli score above the permitted level, and 2 contained coagulase-positive Staphylococcus. Neither Shigella nor Salmonella genus were detected. In the majority of schools studied, it was found that food was of an adequate hygienic-sanitary standard. However, only municipal schools had the supervision of a technician responsible for school food. In the state schools, 60% had never been visited by a nutritionist and in these schools several procedures failed to comply with legal requirements. In most of the schools studied, the food served to students was within adequate standards, though the problems detected revealed the need for the implementation of Best Practices in the school environment.

Streptococcus suis Serotype 2 Biofilms Inhibit the Formation of Neutrophil Extracellular Traps.

Science.gov (United States)

Ma, Fang; Yi, Li; Yu, Ningwei; Wang, Guangyu; Ma, Zhe; Lin, Huixing; Fan, Hongjie

2017-01-01

Invasive infections caused by Streptococcus suis serotype 2 (SS2) has emerged as a clinical problem in recent years. Neutrophil extracellular traps (NETs) are an important mechanism for the trapping and killing of pathogens that are resistant to phagocytosis. Biofilm formation can protect bacteria from being killed by phagocytes. Until now, there have only been a few studies that focused on the interactions between bacterial biofilms and NETs. SS2 in both a biofilm state and a planktonic cell state were incubated with phagocytes and NETs, and bacterial survival was assessed. DNase I and cytochalasin B were used to degrade NET DNA or suppress phagocytosis, respectively. Extracellular DNA was stained with impermeable fluorescent dye to quantify NET formation. Biofilm formation increased up to 6-fold in the presence of neutrophils, and biofilms were identified in murine tissue. Both planktonic and biofilm cells induced neutrophils chemotaxis to the infection site, with neutrophils increasing by 85.1 and 73.8%, respectively. The bacteria in biofilms were not phagocytized. The bactericidal efficacy of NETs on the biofilms and planktonic cells were equal; however, the biofilm extracellular matrix can inhibit NET release. Although biofilms inhibit NETs release, NETs appear to be an important mechanism to eliminate SS2 biofilms. This knowledge advances the understanding of biofilms and may aid in the development of treatments for persistent infections with a biofilm component.

EXPERIENCE SANITARY-EPIDEMIOLOGICAL EXAMINATION PROJECT OF PLACING A SOURCE OF IONIZING RADIATION (GENERATING IN HEALTH CARE INSTITUTIONS

Directory of Open Access Journals (Sweden)

I. A. Rakitin

2015-01-01

Full Text Available The article reviews the results of long-term sanitary-epidemiological examination of projects of placing of ionizing radiation (generating sources in health care institutions of Saint-Petersburg. The majority among the placed sources presented for examination was X-ray diagnostic units and sets – 35.7%, dentist X-rays – 39.4% and fluorography units – 10.8%. Mammography units and computer tomographs made 6.7% each, accelerants – 0.7%.The most frequent reasons of primary refusals to accept design documentation were: absence of calculations of protection against all placed diagnostic X-ray devices (23.6% – at placing of diagnostic X-ray sets, 16.2% – at placing of dentist devices, absence of the upper floors layouts (26.5% – at placing of dentist X-rays and absence of permitting documentation for X-ray devices (at placing of dentist X-ray devices – 22.2%.At carrying out of design activity of special importance is creation of medical and technical projects which were absent in 22.9% of presented projects and in 34.6% were replaced with technical projects. Significant drawbacks of the projects were ignoring the necessity to consider the distance from the personnel workplaces and the width of technical passes (34.0%. That was caused by the absence of corresponding documentation from suppliers of equipment for X-ray rooms.At calculation of protection against X-ray radiation in 11.3% of projects of placing X-ray diagnostic devices (sets and in 7.7% of projects of placing dentist X-ray devices, radiation directivity factors (N were determined incorrectly.Of importance is the issue of adequate choice of building and finishing materials. In 50.0% of projects of placing of diagnostic X-ray devices (sets and 37.6% of projects of placing dentist X-ray devices there were no sanitary-epidemiological conclusions regarding the building and finishing materials to confirming their feasibility for healthcare institutions.Analysis of the main stages

Choice of noxious facilities: case of a solid waste incinerator versus a sanitary landfill in Malaysia.

Science.gov (United States)

Othman, Jamal; Khee, Pek Chuen

2014-05-01

A choice experiment analysis was conducted to estimate the preference for specific waste disposal technologies in Malaysia. The study found that there were no significant differences between the choice of a sanitary landfill or an incinerator. What matters is whether any disposal technology would lead to obvious social benefits. A waste disposal plan which is well linked or integrated with the community will ensure its acceptance. Local authorities will be challenged to identify solid waste disposal sites that are technically appropriate and also socially desirable.

Oesophagostomum dentatum and Trichuris suis infections in pigs born and raised on contaminated paddocks

DEFF Research Database (Denmark)

Mejer, Helena; Roepstorff, Allan Knud

2006-01-01

.p.) the offspring was slaughtered serially every 2 weeks for parasite recovery. Faeces were collected regularly for parasite egg counts and acid-insoluble ash (AIA) content as an indicator of geophagy. Weaning took place week 7 p.p. by removing the sow. Paddock infection levels were estimated in mid-June (O....... dentatum) and late November (O. dentatum and T. suis) using helminth naïve tracer pigs. Soil and vegetation samples were collected regularly. Despite a high initial contamination by the seeder pigs, O. dentatum paddock infectivity was negligible to low throughout the raising of the experimental piglets.......p. The experimental pigs initially had a high faecal level of AIA though it decreased over time. The results are discussed in relation to the biological characteristics of the 2 parasites and their occurrence in organic pig production....

[Legal and sanitary aspects conditioning access to medicines in Brazilian courts].

Science.gov (United States)

Pandolfo, Mércia; Delduque, Maria Célia; Amaral, Rita Goreti

2012-01-01

The search for having access to health care and medicines right granted through Judicial Courts has increased in Brazil. What has been nominated "health judicialization" is a multidimensional phenomenon, a need for dealing with it in a multidisciplinary way involving legal-judicial, political-institutional and sanitary approaches has raised. The Health is recognized as a fundamental human right in the Brazilian Constitution giving it a different legal protection under the legal-constitutional order and the country guarantees the right to health are not only the Constitution and the law strictly, but mainly in an normative infralegal arc that define the goals and outcomes to be achieved by public policy. The lawsuits by drugs may be a reflection of the difficulty of access to health services, to empty and downgrading of health care. Therefore, this turns out to affect the judicialization of pharmaceutical care in Brazil.

Public health assessment for Freeway Sanitary Landfill, Burnsville, Dakota County, Minnesota, Region 5. CERCLIS No. MND038384004. Final report

International Nuclear Information System (INIS)

1993-01-01

The Freeway Sanitary Landfill National Priorities List (NPL) site in Burnsville, Minnesota is situated in the Lower Minnesota River Valley. Shallow groundwater beneath the site is contaminated with low levels of volatile organic hydrocarbons and heavy metals. Under current conditions, no human exposures to site-related contaminants are known to occur at levels of health concern. Based on currently available information, the Minnesota Department of Health concludes that the site poses an indeterminate public health hazard under current conditions because exposure to volatile gases released to the air is possible, but cannot be evaluated from the very limited information available. There are also a few physical hazards on the site which pose a risk of accident or injury if trespassing occurs. Otherwise, there are no indications that people have been, or are being, exposed to site-related contaminants at levels that would be of health concern. The Agency For Toxic Substances and Disease Registry (ATSDR) Health Activities Recommendation Panel has evaluated the Freeway Sanitary Landfill Public Health Assessment for appropriate follow-up activities. The Panel has recommended that health education be considered to assist site workers in better understanding their potential for exposure to landfill gases

Independent Verification Survey Report for the Offsite Portion of the Potential Release Site-7 Abandoned Sanitary Line, Miamisburg Closure Project, Miamisburg, Ohio

International Nuclear Information System (INIS)

Weaver, P.C.

2008-01-01

The ORISE objective was to confirm that the remedial action process implemented by the contractor was in accordance with the PRS-7 Work Package. Following removal of the sanitary line, the soil beneath the line would be sampled to determine if remediation was required (ARC 2007a).

Diagnosis of porcine enzootic pneumonia by post mortem sanitary inspection: comparison with other diagnostic methods

OpenAIRE

Kênia de Fátima Carrijo; Elmiro Rosendo do Nascimento; Virginia Léo de Almeida Pereira; Nelson Morés; Catia Silene Klein; Leonardo Muliterno Domingues; Rogerio Tortelly

2014-01-01

ABSTRACT. Carrijo K.F., Nascimento E.R., Pereira V.L.A., Morés N., Klein, C.S., Domingues L.M. & Tortelly R. [Diagnosis of porcine enzootic pneumonia by post mortem sanitary inspection: comparison with other diagnostic methods.] Diagnóstico da pneumonia enzoótica suína pela inspeção sanitária post mortem: comparação com outros métodos de diagnóstico. Revista Brasileira de Veterinária Brasileira 36(2):188-194, 2014. Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Par...

Sanitary impact of the particulate atmospheric urban pollution; Impact sanitaire de la pollution atmospherique urbaine particulaire

Energy Technology Data Exchange (ETDEWEB)

Sentissi, M.

1999-03-22

The pollution of particulates origin is one of the principle actual problem relative to air quality. In France, the fine particulates come from industry and automobile traffic, especially, the diesel vehicles. The most worrying characteristic is their fineness, that allow them to stay in suspension during a long time and penetrate into pulmonary alveoli, with toxic elements at their surface such metals, acids, polycyclic aromatic hydrocarbons. The objective of this work is to take stock of epidemiology and toxicology studies evaluating the sanitary impact of particulates in suspension. (N.C.)

Environmental review report of an electrical generation facility to be located at the Oaks Sanitary Landfill

International Nuclear Information System (INIS)

Goldstein, D.; Ross, J.; Mountain, D.; Kahal, M.

1998-05-01

The Bentech Group, Inc. (Bentech) applied for a certificate of public convenience and necessity (CPCN) to construct and operate an electric generating system at the Oaks Sanitary Landfill in Laytonsville, Maryland. The focus of the environmental review is to evaluate potential impacts of the proposed electric generation system to air quality, noise, terrestrial, ecological, ground water, surface water, socioeconomic, aesthetic, and cultural resources. This document presents the results of the environmental review analysis, and includes the State's recommended license conditions for operating the electric generating system, which the PSC incorporated into the CPCN

Report: the current situation of sanitary landfills in Brazil and the importance of the application of economic models

OpenAIRE

Oliveira Neto, Raúl; Otávio Petter, Carlos; Cortina Pallás, José Luís

2009-01-01

We present the development stage of the sanitary landfills in Brazil in the context of urban solid residue management, demonstrating the necessity and importance of the employment of economic models. In the article, a cost estimate model is proposed as the basis for studies to be applied by sector management, including the city council, companies, consultants and engineers, contributing to the choice of new areas, public bids, municipal consortia and private public partnerships. Peer Re...

Collection of main international documents on the ethical principles of research and the main regulations on bioethics of the United States and of European Community; Raccolta sui principali documenti internazionali sui principi etici della ricerca e dei principali documenti legislativi degli Stati Uniti e della Comunita` Europea sulla bioetica

Energy Technology Data Exchange (ETDEWEB)

Scuderi, G. [Istituto Superiore di Sanita`, Rome (Italy). Lab. di Immunologia

1998-12-31

The collection includes an Italian translation of the main international documents on ethical principles of scientific research (the Nuremberg Code, the Helsinki Declaration, the Belmont Report) as well as the regulatory text of the United States (the Code of Federal Regulation Title 45, Part 46, that is the 45 Cfr 46) and the regulations of the European Community (the Good Clinical Practice and the Convention for the protection of human rights and dignity of the human being regards to the application of biology and medicine: convention on human rights and biomedicine). The informed consent and the human rights of human subjects involved in a clinical trial are discussed. Moreover, in the regulatory texts, legal rights of particular subjects, i.e. pregnant women, minors, fetuses, embrions, prisoners, are discussed; as well as the bioethics of organ transplantations. [Italiano] Contiene una traduzione in lingua italiana dei principali documenti internazionali sui principi etici della ricerca scientifica: il Codice di Norimberga, la dichiarazione di Helsinki e il rapporto Belmont; il testo legislativo degli Stati Uniti Code of Federal Regulation, capitolo 45 parte 46 (detto il 45 CFR 46) e la normativa della Comunita` Europea relativa alle norme di Good Clinical Practice e alla Convenzione per la protezione dei diritti dell`uomo e la dignita` dei soggetti umani relativamente alle applicazioni della biologia e medicina: Convenzione sui diritti dell`uomo e la biomedicina. Vengono trattati il consenso informato e i diritti umani delle persone che sono coinvolte in una sperimentazione clinica. Inoltre, nei testi normativi ci si riferisce anche ai diritti legali di soggetti particolari, ad esempio: donne in stato di gravidanza, minori, feti, embrioni, prigionieri; viene trattata, infine, l`etica dei trapianti d`organo.

Collection of main international documents on the ethical principles of research and the main regulations on bioethics of the United States and of European Community; Raccolta sui principali documenti internazionali sui principi etici della ricerca e dei principali documenti legislativi degli Stati Uniti e della Comunita` Europea sulla bioetica

Energy Technology Data Exchange (ETDEWEB)

Scuderi, G [Istituto Superiore di Sanita` , Rome (Italy). Lab. di Immunologia

1999-12-31

The collection includes an Italian translation of the main international documents on ethical principles of scientific research (the Nuremberg Code, the Helsinki Declaration, the Belmont Report) as well as the regulatory text of the United States (the Code of Federal Regulation Title 45, Part 46, that is the 45 Cfr 46) and the regulations of the European Community (the Good Clinical Practice and the Convention for the protection of human rights and dignity of the human being regards to the application of biology and medicine: convention on human rights and biomedicine). The informed consent and the human rights of human subjects involved in a clinical trial are discussed. Moreover, in the regulatory texts, legal rights of particular subjects, i.e. pregnant women, minors, fetuses, embrions, prisoners, are discussed; as well as the bioethics of organ transplantations. [Italiano] Contiene una traduzione in lingua italiana dei principali documenti internazionali sui principi etici della ricerca scientifica: il Codice di Norimberga, la dichiarazione di Helsinki e il rapporto Belmont; il testo legislativo degli Stati Uniti Code of Federal Regulation, capitolo 45 parte 46 (detto il 45 CFR 46) e la normativa della Comunita` Europea relativa alle norme di Good Clinical Practice e alla Convenzione per la protezione dei diritti dell`uomo e la dignita` dei soggetti umani relativamente alle applicazioni della biologia e medicina: Convenzione sui diritti dell`uomo e la biomedicina. Vengono trattati il consenso informato e i diritti umani delle persone che sono coinvolte in una sperimentazione clinica. Inoltre, nei testi normativi ci si riferisce anche ai diritti legali di soggetti particolari, ad esempio: donne in stato di gravidanza, minori, feti, embrioni, prigionieri; viene trattata, infine, l`etica dei trapianti d`organo.

Sanitary risks related to the installation of hydroelectric turbines on drinking water networks

International Nuclear Information System (INIS)

Novelli, A.; Montiel, A.; Cabillic, P.J.; Fourrier, P.; Levi, Y.; Potelon, J.L.; Welte, B.; Fourrier, P.; Levi, Y.; Potelon, J.L.; Welte, B.

2010-01-01

With the notion of sustainable development gaining ground, practices aimed at saving water and energy are more and more frequent, particularly the installation of hydroelectric turbine on drinking water networks. It is essential in this case that the water quality should not be deteriorated, and the water supply for consumption and fire protection has to be prioritized over energy production. Thus, a sanitary risk assessment must be done and actions to control the described critical points have to be taken. The installation of a turbine is an additional risk whereas it is not necessary for drinking water production and distribution. As a consequence, a quality management system including the turbine and additional quality water monitoring should be carried out. (authors)

EXPERIENCE SANITARY-EPIDEMIOLOGICAL EXAMINATION PROJECT OF PLACING A SOURCE OF IONIZING RADIATION (GENERATING) IN HEALTH CARE INSTITUTIONS

OpenAIRE

I. A. Rakitin; A. L. Zel’din; V. B. Karpov

2015-01-01

The article reviews the results of long-term sanitary-epidemiological examination of projects of placing of ionizing radiation (generating) sources in health care institutions of Saint-Petersburg. The majority among the placed sources presented for examination was X-ray diagnostic units and sets – 35.7%, dentist X-rays – 39.4% and fluorography units – 10.8%. Mammography units and computer tomographs made 6.7% each, accelerants – 0.7%.The most frequent reasons of primary refusals to accept des...

The role of industrial-sanitary laboratory of medical aid station of Federal administration in prophylaxis of emergency situations at the plants of nuclear-energy complex and liquidation of their after-effects

International Nuclear Information System (INIS)

Antipin, E.B.

1995-01-01

On the example of several radiation accidents the author summarized the personal practical experience in organization of work in industrial-sanitary laboratories based at the nuclear energy plants of nuclear-energy complex, in respect of prophylaxis of emergency radiation situations and liquidation of their after-effects. It is pointed out that successful activity in rendering emergency medical aid in emergency situations, caused by radiation accidents, is possible only subject to close contact of science, practical medical care and sanitary service which should become a part of the uniform public health system. 4 refs

Serological patterns of Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae, Pasteurella multocida and Streptococcus suis in pig herds affected by pleuritis.

Science.gov (United States)

Wallgren, Per; Nörregård, Erik; Molander, Benedicta; Persson, Maria; Ehlorsson, Carl-Johan

2016-10-04

Respiratory illness is traditionally regarded as the disease of the growing pig, and has historically mainly been associated to bacterial infections with focus on Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. These bacteria still are of great importance, but continuously increasing herd sizes have complicated the scenario and the influence of secondary invaders may have been increased. The aim of this study was to evaluate the presence of A. pleuropneumoniae and M. hyopneumoniae, as well as that of the secondary invaders Pasteurella multocida and Streptococcus suis by serology in four pig herds (A-D) using age segregated rearing systems with high incidences of pleuritic lesions at slaughter. Pleuritic lesions registered at slaughter ranged from 20.5 to 33.1 % in the four herds. In herd A, the levels of serum antibodies to A. pleuropneumoniae exceeded A 450  > 1.5, but not to any other microbe searched for. The seroconversion took place early during the fattening period. Similar levels of serum antibodies to A. pleuropneumoniae were also recorded in herd B, with a subsequent increase in levels of antibodies to P. multocida. Pigs seroconverted to both agents during the early phase of the fattening period. In herd C, pigs seroconverted to P. multocida during the early phase of the fattening period and thereafter to A. pleuropneumoniae. In herd D, the levels of antibodies to P. multocida exceeded A 450  > 1.0 in absence (A 450  hyopneumoniae and to S. suis remained below A 450  hyopneumoniae late during the rearing period (herd B-D), or not at all (herd A). Different serological patterns were found in the four herds with high levels of serum antibodies to A. pleuropneumoniae and P. multocida, either alone or in combination with each other. Seroconversion to M. hyopneumoniae late during the rearing period or not at all, confirmed the positive effect of age segregated rearing in preventing or delaying infections with M

L'applicazione dei Sistemi Informativi Geografici per la valutazione dei bisogni di salute della popolazione pediatrica immigrata residente a Roma

Directory of Open Access Journals (Sweden)

A. G. De Belvis

2003-05-01

Full Text Available

Obiettivi: lo studio applica i GIS per l’analisi dei bisogni di salute della popolazione immigrata in età pediatrica regolarmente residente a Roma e per la costruzione di bacini di utenza a livello di Municipio, ASL e Comune rispetto alle corrispondenti strutture di offerta.

Materiali e Metodi: i dati sono stati acquisiti dall’Ufficio Statistica del Comune di Roma (popolazione in età 0-18 anni residente al 31/12/2002 per Municipio, Distretto e ASL. I dati relativi all’offerta dei servizi per l’infanzia delle ASL sono stati reperiti dalle ASL, dal Comune e dalla Caritas di Roma. I dati sono stati successivamente inseriti, verificati ed analizzati attraverso i programmi Excel e Stata7. L’utilizzo delle applicazioni GIS è stato realizzato attraverso il software Arc-View.

Risultati: dei 169.064 immigrati presenti al 31/12/2002, quelli in età pediatrica (29.629 sono variabilmente distribuiti rispetto al totale degli immigrati e alla popolazione pediatrica residente.
Ad esempio, la proporzione immigrati minori/immigrati varia da un minimo del 7,3% in Municipio I, ad un massimo del 20,1% nei Municipi VII e VII (Media: 13.26, I.C.95%: 11.46-15.05.
L’analisi ha permesso di caratterizzare i bisogni socio-sanitari per Municipio, distretto e ASL. Una valutazione quantitativa della relazione bisogno/offerta è stata realizzata georeferenziando la popolazione immigrata in età pediatrica con le corrispondenti strutture di offerta censite.

Conclusioni: l’utilizzo dei GIS in epidemiologia e sanità pubblica si presenta sempre più prezioso, per la capacità di sintetizzare, rappresentare efficacemente i fenomeni, suggerire ipotesi di correlazioni tra i fenomeni e indirizzare le decisioni in sanità.
L’applicazione dei GIS sulla popolazione immigrata in età pediatrica – regolarmente residente a Roma - si è prestata in maniera efficace

Life cycle analysis of sanitary landfill and incineration of municipal solid waste

Institute of Scientific and Technical Information of China (English)

倪晋仁; 韦洪莲; 刘阳生; 赵智杰

2002-01-01

Environmental consequences from sanitary landfill as well as incineration with power generation were compared in terms of life cycle analysis (LCA) for Laohukeng Waste-disposal Plant that is under consideration in Shenzhen. A variety of differences will be resulted from the two technologies, from which the primary issue that affects the conclusion is if the compensatory phase in power generation can be properly considered in the boundary definition of LCA. Upon the compensatory phase is taken into account in the landfill system, the negative environmental consequences from the landfill will be more significant than those from the incineration with power generation, although the reversed results can be obtained as the compensatory phase is neglected. In addition, mitigation of environmental impacts through the pollutant treatment in the incineration process will be more effective than in the landfill process.

Use of Nuclear Techniques to Plan Sanitary Strategies in the Almendares River

International Nuclear Information System (INIS)

Dominguez Catasus, Judith; Borroto Portela, Jorge; Hernandez Garces, Anel; Santiago, Jose F.

2003-01-01

The Almendares river, one of the most important water bodies of the Havana City, is very polluted. The analysis of parameters as dissolved oxygen, biochemical oxygen demand and ammonium is very helpful for the studies aimed to the recovery of the river. There is a growing recognition around the word that the water quality models are very useful tools to plan sanitary strategies for the management of wastewater contamination to predict the effectiveness of control options to improve water quality to desired levels. In the present work, the advective, steady- state, one-dimensional Streeter and Phelps model, calibrated and validated was used to elaborate environment strategies to improve the water quality of the Almendares river. The use of the 99mTc and the Rodamina WT as tracers allowed determining the hydrodynamic parameters necessary for modelling purposes. (Author)

Sanitary survey of the drinking water supply of Kombinati suburb-Tirana, Albania.

Science.gov (United States)

Angjeli, V; Reme, B; Leno, L; Bukli, R; Bushati, G

2000-01-01

Microbiological pollution of drinking water is a major health problem in the suburbs of the Albanian capital. Intermittent supply and contamination, resulting in several gastrointestinal manifestations, are the main concerns for the population and health workers. The risk of outbreaks of water-borne diseases is high. Pollution originates from contamination of drinking water with domestic sewage. This research investigated the drinking water cycle from its natural source to the consumer, analysing samples and verifying pollution levels in the microbiological and chemical setting. The most important pollution sources were found in the distribution network, due to cross-contamination with sewers and illegal connections. The second pollution source was found around the extraction wells. This is related to abusive constructions within the sanitary zone around the wells and maybe the highly sewage-contaminated river water which feeds the aquifer.

Records of performance and sanitary status from a dairy cattle herd in southern Brazil

Directory of Open Access Journals (Sweden)

Cláudio E. F. Cruz

2011-01-01

Full Text Available Over the last decades, the emphasis on the health of dairy cows has changed from an individual to a herd level. In this scenario, the role played by the recording system and its interpretation by veterinarians has gained primordial importance. The records of productive and reproductive performance and of sanitary status from a southern Brazilian dairy cattle herd have been presented and discussed. The period of study was 2000-2009. Mean values per lactation period were 349D 8436M 290F 275P 201SCS (D: days in lactation, M: kg of milk yield, F: kg of fat, P: kg of protein and SCS: somatic cell score in 1000 cells/ml of milk. Major indexes of reproductive efficiency included age at first calving (31 months, services per conception (2.1, intercalving interval (428 days, calving to conception interval (146 days, mean annual rates of parturitions (76.2%, fetal losses (9.8-19.0%, and stillbirths (3.6%, apart of voluntary waiting period (94 days. Main information on sanitary status of the herd was associated with the mean prevalence of common disorders of dairy cattle such as anaplasmosis (29.8%, mastitis (27.8%, digital diseases (26.3%, ovarian cysts (21.3%, placental retention (19.7%, postpartum uterine infections (10.6%, and calf diarrhea (23.7% and pneumonia (16.8%, among others. In addition, culling reasons (low reproductive performance [56.3%] and udder/mastitis problems [33.6%], causes of cattle deaths (anaplasmosis [16.4%] and leukosis [11.4], and the impact of cattle diseases such as tuberculosis, leukosis, and neosporosis on the herd have also been presented and succinctly discussed. Numbers between brackets represent rates accumulated in the 10-year period.

33 CFR 165.923 - Regulated Navigation Area between mile markers 296.1 and 296.7 of the Chicago Sanitary and Ship...

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Regulated Navigation Area between mile markers 296.1 and 296.7 of the Chicago Sanitary and Ship Canal located near Romeoville, IL. 165.923 Section 165.923 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED...

Reconcentration of radioactive material released to sanitary sewers in accordance with 10 CFR Part 20

International Nuclear Information System (INIS)

Ainsworth, C.C.; Hill, R.L.; Cantrell, K.J.; Kaplan, D.I.; Norton, M.V.; Aaberg, R.L.; Stetar, E.A.

1994-12-01

The US Nuclear Regulatory Commission (NRC), in accordance with 10 CFR 20, and agreement states, in accordance with state regulations, regulates the discharge of radioactive materials into sanitary sewer systems. A one-year study was conducted by Pacific Northwest Laboratory (PNL) for the NRC to assess whether radioactive materials that are dischared to sanitary sewer systems undergo significant reconcentration within the wastewater treatment plants (WWTP) and to determine the physical and/or chemical processes that may result in radionuclide reconcentration within the WWTPs. The study objectives were addressed by collecting information and data on wastewater treatment, relevant geochemical processes, and individual radionuclide behavior in WWTPs from the open literature, NRC reports, EPA surveys, and interviews with NRC licensees and staff of WWTPs that may be impacted by these discharges. Radionuclide mass balance and removal efficiencies were calculated for WWTPs at Oak Ridge, TN; and Erwin, TN, but were not shown to be reliable since the licensee release data generally underestimated the mass of radionuclide that was ultimately found in the sludge. This disparity may be due, in part, to the fact that data available for use in this study were collected to address regulatory concerns and not to perform mass balance calculations. A limited modeling study showed some promise for predicting radionuclide behavior in WWTPS, however, the general applicability of using these empirical models remains uncertain. With the data and models currently available, it is not possible to quantitatively determine the physical and chemical processes that cause reconcentration or to calculate, a priori, reconcentration factors for specific WWTP unit processes or WWTPs in general

Retention of Employees in Ceramic Sanitary Ware Factories in India: Role of Work Life Balance, Career Development and Supervisor Support

OpenAIRE

S. Umamaheswari; Jayasree Krishnan

2016-01-01

The study examines the role of work life balance, career development and supervisor support on organization commitment over employees of unattended, ceramic sanitary ware factories in India. It also verifies the influence of organization commitment on retention and its mediating role. Findings reveal that organization commitment influences retention and all the above factors enhance it. Moreover, organization commitment partially mediates the relationship between proposed factors and retentio...

Problems of optimization of activities of sanitary and epidemiological stations on radiation protection

International Nuclear Information System (INIS)

Poplavskij, K.K.

1989-01-01

Problems of activity optimization of the sanitary and epidemiologic stations (SES) concerning state inspection of for radiation source application are considered to improve the effort efficiency of the radiological subdivisions. The necessity to specify the inspection objects is shown. Inspection of all the stages of creation, introduction and application of radioactive substances and other sources, as well as, of radioactive waste utilization remains urgent problem. Determination of internal and external radiation doses to population in different regions as well as, radiation protection of personnel and patients in nuclear medicine are vital problems as well. Justification of the necessity to enlist specialists in different fields, to determine rationally their functional duties presents sufficient component of the SES activity optimization. Usage optimization of dosimetric and radiometric devices, laboratory equipment and instruments is a vital problem

Social life and sanitary risks: evolutionary and current ecological conditions determine waste management in leaf-cutting ants.

Science.gov (United States)

Farji-Brener, Alejandro G; Elizalde, Luciana; Fernández-Marín, Hermógenes; Amador-Vargas, Sabrina

2016-05-25

Adequate waste management is vital for the success of social life, because waste accumulation increases sanitary risks in dense societies. We explored why different leaf-cutting ants (LCA) species locate their waste in internal nest chambers or external piles, including ecological context and accounting for phylogenetic relations. We propose that waste location depends on whether the environmental conditions enhance or reduce the risk of infection. We obtained the geographical range, habitat and refuse location of LCA from published literature, and experimentally determined whether pathogens on ant waste survived to the high soil temperatures typical of xeric habitats. The habitat of the LCA determined waste location after phylogenetic correction: species with external waste piles mainly occur in xeric environments, whereas those with internal waste chambers mainly inhabit more humid habitats. The ancestral reconstruction suggests that dumping waste externally is less derived than digging waste nest chambers. Empirical results showed that high soil surface temperatures reduce pathogen prevalence from LCA waste. We proposed that LCA living in environments unfavourable for pathogens (i.e. xeric habitats) avoid digging costs by dumping the refuse above ground. Conversely, in environments suitable for pathogens, LCA species prevent the spread of diseases by storing waste underground, presumably, a behaviour that contributed to the colonization of humid habitats. These results highlight the adaptation of organisms to the hygienic challenges of social living, and illustrate how sanitary behaviours can result from a combination of evolutionary history and current environmental conditions. © 2016 The Author(s).

Evaluation of sanitary impact of urban air pollution. Methodological guide.; Evaluation de l'impact sanitaire de la pollution atmospherique urbaine. Guide methodologique

Energy Technology Data Exchange (ETDEWEB)

Glorennec, Ph.; Nourry, L [Cellule Inter-Regionale d' Epidemiologie (France); Quenel, Ph. [Institut National de Veille Sanitaire, 94 - Saint Maurice (France)] [and others

1999-07-01

The institute of sanitary watch has setting up an epidemiological surveillance plan in nine french towns in order to quantify and to keep a close eye to the relations existing between the urban air pollution and the populations health.The method is detailed with its advantages and its limits. (N.C.)

Palytoxin-Containing Aquarium Soft Corals as an Emerging Sanitary Problem

Directory of Open Access Journals (Sweden)

Marco Pelin

2016-02-01

Full Text Available Palytoxin (PLTX, one the most potent marine toxins, and/or its analogs, have been identified in different marine organisms, such as Palythoa soft corals, Ostreopsis dinoflagellates, and Trichodesmium cyanobacteria. Although the main concern for human health is PLTXs entrance in the human food chain, there is growing evidence of adverse effects associated with inhalational, cutaneous, and/or ocular exposure to aquarium soft corals contaminated by PLTXs or aquaria waters. Indeed, the number of case reports describing human poisonings after handling these cnidarians is continuously increasing. In general, the signs and symptoms involve mainly the respiratory (rhinorrhea and coughing, skeletomuscular (myalgia, weakness, spasms, cardiovascular (electrocardiogram alterations, gastrointestinal (nausea, and nervous (paresthesia, ataxia, tremors systems or apparates. The widespread phenomenon, the entity of the signs and symptoms of poisoning and the lack of control in the trade of corals as aquaria decorative elements led to consider these poisonings an emerging sanitary problem. This review summarizes literature data on human poisonings due to, or ascribed to, PLTX-containing soft corals, focusing on the different PLTX congeners identified in these organisms and their toxic potential.

[Using modern information technology in the practice of the sanitary-epidemiological surveiliance during the XXII Olympic Winter Games and XI Paralympic Winter Games in Sochi].

Science.gov (United States)

Popova, A Yu; Kuzkin, B P; Demina, Yu V; Dubyansky, V M; Kulichenko, A N; Maletskaya, O V; Shayakhmetov, O Kh; Semenko, O V; Nazarenko, Yu V; Agapitov, D S; Mezentsev, V M; Kharchenko, T V; Efremenko, D V; Oroby, V G; Klindukhov, V P; Grechanaya, T V; Nikolaevich, P N; Tesheva, S Ch; Rafeenko, G K

2015-01-01

To improve the sanitary and epidemiological surveillance at the Olympic Games has developed a system of GIS for monitoring objects and situations in the region of Sochi. The system is based on software package ArcGIS, version 10.2 server, with Web-java.lang. Object, Web-server Apach, and software developed in language java. During th execution of the tasks are solved: the stratification of the region of the Olympic Games for the private and aggregate epidemiological risk OCI various eti- ologies, ranking epidemiologically important facilities for the sanitary and hygienic conditions, monitoring of infectious diseases (in real time according to the preliminary diagnosis). GIS monitoring has shown its effectiveness: Information received from various sources, but focused on one portal. Information was available in real time all the specialists involved in ensuring epidemiological well-being and use at work during the Olympic Games in Sochi.

Servizi finanziari imputati e interdipendenze settoriali: un'analisi settoriale del ruolo del credito nel sistema economico. (Imputed bank services and sectoral interdependences: a structural analysis of the role of credit in the economy

Directory of Open Access Journals (Sweden)

C. BIANCHI

2013-12-01

Full Text Available I sistemi di contabilità nazionale in base alla metodologia SEC sono soliti comportarsi in modo da garantire l'impossibilità pratica di effettuare qualsiasi assegnazione significativa di servizi bancari imputati tra i singoli rami di attività economica.  Il presente lavoro mostra come questo vieta l'analisi strutturale del ruolo del credito nel sistema di interdipendenze. . L'analisi mette in evidenza la duplice natura del credito come contenuti a valore aggiunto altamente intermedio e alto. È in grado di influenzare forte su i costi di produzione degli altri rami, senza essere influenzato da loro. Queste proprietà conferiscono al settore bancario un potenziale molto elevato per l'inflazione.National accounts systems based on the SEC methodology are usually thought to comport the practical impossibility of carrying out any meaningful allocation of imputed bank services among the single branches of economic activity. As a consequence, the total value of the net interest earned by the credit system as a whole is considered as a cost entry and a negative component of added value in an ad-hoc additional industry, to be aggregated to the main credit one in the typical input-output analysis. The present work shows how this prohibits the structural analysis of the role of credit in the system of interdependencies. A method of is proposed in which imputed services of credit are distributed by branches, on the basis of existing statistics, proving valuable in assessing the significance of certain quantities of national accounts, such as operating results. The analysis highlights the dual nature of credit as highly intermediate and high value-added content. It is able to strongly influence the production costs of the other branches, without being influenced by them. These properties give the banking industry a very high potential for inflation. JEL: E51, G21

The broiler meat system in Nairobi, Kenya: Using a value chain framework to understand animal and product flows, governance and sanitary risks.

Science.gov (United States)

Carron, Maud; Alarcon, Pablo; Karani, Maurice; Muinde, Patrick; Akoko, James; Onono, Joshua; Fèvre, Eric M; Häsler, Barbara; Rushton, Jonathan

2017-11-01

Livestock food systems play key subsistence and income generation roles in low to middle income countries and are important networks for zoonotic disease transmission. The aim of this study was to use a value chain framework to characterize the broiler chicken meat system of Nairobi, its governance and sanitary risks. A total of 4 focus groups and 8 key informant interviews were used to collect cross-sectional data from: small-scale broiler farmers in selected Nairobi peri-urban and informal settlement areas; medium to large integrated broiler production companies; traders and meat inspectors in live chicken and chicken meat markets in Nairobi. Qualitative data were collected on types of people operating in the system, their interactions, sanitary measures in place, sourcing and selling of broiler chickens and products. Framework analysis was used to identify governance themes and risky sanitary practices present in the system. One large company was identified to supply 60% of Nairobi's day-old chicks to farmers, mainly through agrovet shops. Broiler meat products from integrated companies were sold in high-end retailers whereas their low value products were channelled through independent traders to consumers in informal settlements. Peri-urban small-scale farmers reported to slaughter the broilers on the farm and to sell carcasses to retailers (hotels and butcheries mainly) through brokers (80%), while farmers in the informal settlement reported to sell their broilers live to retailers (butcheries, hotels and hawkers mainly) directly. Broiler heads and legs were sold in informal settlements via roadside vendors. Sanitary risks identified were related to lack of biosecurity, cold chain and access to water, poor hygiene practices, lack of inspection at farm slaughter and limited health inspection in markets.
 Large companies dominated the governance of the broiler system through the control of day-old chick production. Overall government control was described as

US EPA record of decision review for landfills: Sanitary landfill (740-G), Savannah River Site

Energy Technology Data Exchange (ETDEWEB)

1993-06-01

This report presents the results of a review of the US Environmental Protection Agency (EPA) Record of Decision System (RODS) database search conducted to identify Superfund landfill sites where a Record of Decision (ROD) has been prepared by EPA, the States or the US Army Corps of Engineers describing the selected remedy at the site. ROD abstracts from the database were reviewed to identify site information including site type, contaminants of concern, components of the selected remedy, and cleanup goals. Only RODs from landfill sites were evaluated so that the results of the analysis can be used to support the remedy selection process for the Sanitary Landfill at the Savannah River Site (SRS).

US EPA record of decision review for landfills: Sanitary landfill (740-G), Savannah River Site

International Nuclear Information System (INIS)

1993-06-01

This report presents the results of a review of the US Environmental Protection Agency (EPA) Record of Decision System (RODS) database search conducted to identify Superfund landfill sites where a Record of Decision (ROD) has been prepared by EPA, the States or the US Army Corps of Engineers describing the selected remedy at the site. ROD abstracts from the database were reviewed to identify site information including site type, contaminants of concern, components of the selected remedy, and cleanup goals. Only RODs from landfill sites were evaluated so that the results of the analysis can be used to support the remedy selection process for the Sanitary Landfill at the Savannah River Site (SRS)

MLVA genotyping of Brucella melitensis and Brucella abortus isolates from different animal species and humans and identification of Brucella suis vaccine strain S2 from cattle in China.

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Hai Jiang

Full Text Available In China, brucellosis is an endemic disease and the main sources of brucellosis in animals and humans are infected sheep, cattle and swine. Brucella melitensis (biovars 1 and 3 is the predominant species, associated with sporadic cases and outbreak in humans. Isolates of B. abortus, primarily biovars 1 and 3, and B. suis biovars 1 and 3 are also associated with sporadic human brucellosis. In this study, the genetic profiles of B. melitensis and B. abortus isolates from humans and animals were analyzed and compared by multi-locus variable-number tandem-repeat analysis (MLVA. Among the B. melitensis isolates, the majority (74/82 belonged to MLVA8 genotype 42, clustering in the 'East Mediterranean' group. Two B. melitensis biovar 1 genotype 47 isolates, belonging to the 'Americas' group, were recovered; both were from the Himalayan blue sheep (Pseudois nayaur, a wild animal. The majority of B. abortus isolates (51/70 were biovar 3, genotype 36. Ten B. suis biovar 1 field isolates, including seven outbreak isolates recovered from a cattle farm in Inner Mongolia, were genetically indistinguishable from the vaccine strain S2, based on MLVA cluster analysis. MLVA analysis provided important information for epidemiological trace-back. To the best of our knowledge, this is the first report to associate Brucella cross-infection with the vaccine strain S2 based on molecular comparison of recovered isolates to the vaccine strain. MLVA typing could be an essential assay to improve brucellosis surveillance and control programs.

Evaluation of the residual sanitary risk for people spending time on the beaches polluted by the Erika fuel, after the depollution

International Nuclear Information System (INIS)

Dor, F.; Gourier-Frery, C.; Zmirou, D.; Cicolella, A.; Bonnard, R.; Dujardin, R.

2004-01-01

This study aims to evaluate the short dated and long dated sanitary risks for adults and children who are going to spend time on the beach affected by the Erika fuel, after the depollution. 36 beaches were selected and 7 beaches which were not polluted, were taking as a reference. The methodology, the analysis of the data and the results are described. (A.L.B.)

The determination of engineering parameters for the sanitary landfill, Savannah River Site

International Nuclear Information System (INIS)

McMullin, S.R.; Smalley, R.C.; Flood, P.J.

1993-01-01

The Savannah River Site is a 315 square mile, Department of Energy production facility located in western South Carolina. This facility has multiple operational areas which generate a variety of waste materials. Over the nearly 40 years of operation, sanitary wastes were deposited in a 60-acre, permitted solid waste disposal facility located on the site. Refuse and other clean wastes were deposited in shallow, slit trenches, ranging in size from 20 to 50 feet-wide and approximately 400 feet long. The historical depth of deposition appears to range between 12 and 15 feet below the ground surface. Recent changes in regulations has classified some wastes contained within the landfill as hazardous wastes, necessitating the closure of this facility as a RCRA hazardous waste management facility. The focus of this paper is to present the innovative techniques used to fully determine the engineering parameters necessary to reasonably predict future settlements, for input into the closure system design

PHYSIOLOGICAL AND SANITARY QUALITIES OF MAIZE LANDRACE SEEDS STORED UNDER TWO CONDITIONS

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Raquel Stefanello

2015-08-01

Full Text Available The preservation of seed quality during the storage period depends not only on the conditions during production and harvesting but also on the storage and maintenance of appropriate storage product conditions. Thus, the aim of this study was to evaluate the physiological and sanitary qualities of maize landrace seeds stored under two conditions. The maize seed batch varieties Oito carreiras, Cabo roxo and Lombo baio were used. Tests included germination, first count, cold test, accelerated aging and sanity. Based on the results it was concluded that the physiological quality of these seed varieties decreased with the storage period. The major fungi identified in the maize seeds during storage were from the genera Aspergillus, Fusarium and Penicillium, which caused deterioration and reduction of the physiological quality. Storage using a paper bag at a temperature of 10 °C did not prevent the deterioration of maize seeds but was more effective at preserving the quality of the seed compared with a plastic bag at room temperature.

Facility Management personalizzato per realtà complesse

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Autodesk Italia Autodesk Italia

2007-04-01

Full Text Available Il mercato dei servizi oggi è caratterizzato da una crescente complessità; in particolare il mondo dell’Information & Communication Technology si trova di fronte a un processo di vera e propria trasformazione del concetto di servizio: dalla semplice manutenzione hardware e software si è passati alla presa in carico di attività che un tempo erano affidate a fornitori diversi. Le aziende di global services necessitano quindi di informazioni aggiornate e complete, per poter rispondere tempestivamente e con efficacia alle richieste delle aziende. In qualità di struttura istituzionale di supporto all’attività del Presidente del Consiglio, la Presidenza del Consiglio dei Ministri aveva la necessità di realizzare un sistema informativo che supportasse i propri fornitori nell’erogazione dei servizi, in particolare nell’espletamento del contratto siglato per la manutenzione degli edifici e nell’ottimizzazione della manutenzione di questi servizi. Questi ultimi includono manutenzioni di carattere ordinario e straordinario, che vanno dalla sostituzione di una lampadina, alla tinteggiatura, al ripristino di un affresco o di un fregio artistico, alla manutenzione del verde sia all’esterno che all’interno degli edifici della Presidenza del Consiglio.

Optimal sulfur amino acid to lysine ratio for post weaning piglets reared under clean or unclean sanitary conditions

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Roselyn Kahindi

2017-12-01

Full Text Available Two 14-day experiments, each with 90 (Duroc × [Yorkshire × Landrace]; 7.3 ± 0.6 kg piglets, were conducted to determine the optimum sulfur amino acid (SAA to lysine (Lys ratio (SAA:Lys for piglets when reared under clean or unclean sanitary conditions using performance and non-performance response criteria. Piglets were randomly assigned to the following dietary treatments. The basal diet contained 1.18% standardized ileal digestible (SID Lys, and the SAA:Lys was 52%. In diets 2 to 5, the basal diet was supplemented with 4 graded levels of dl-Met to make SAA:Lys of 56%, 60%, 64% and 68%. In Exp. 1, piglets were housed in disinfected clean room. In Exp. 2, piglets were housed in a room previously occupied by other pigs and was not disinfected. On the last day, blood was collected to measure plasma urea nitrogen (PUN and one pig per pen was euthanized to collect jejunal tissue to measure villus height (VH, crypt depth (CD, and VH:CD. In Exp. 1, increasing SAA:Lys linearly and quadratically increased VH and VH:CD (P < 0.05. In Exp. 2, increasing SAA:Lys linearly increased (P < 0.05 VH and VH:CD and linearly and quadratically decreased PUN (P < 0.05. Estimated PUN and VH-based optimum SAA:Lys requirements for clean and unclean sanitary condition were 60%, 63% and 66%, respectively.

Environmental Assessment for the centralization and upgrading of the sanitary wastewater system at the Savannah River Site

International Nuclear Information System (INIS)

1993-09-01

The Department of Energy (DOE) has prepared an environmental assessment for the proposed centralization and upgrading of the sanitary wastewater system on the Savannah River Site (SRS), near Aiken, proposed action is not a major Federal action significantly affecting the South Carolina. Based on the analyses in the EA, DOE has determined that the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an environmental impact statement is not required, and DOE is issuing this Finding of No Significant Impact and Floodplain Statement of Findings

Le prime sensazioni al pianoforte restano per sempre? Indagine sui metodi pianistici per principianti

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Giuseppe Sellari

2015-12-01

Full Text Available I metodi d’insegnamento del pianoforte utilizzati in Italia sono caratterizzati da percorsi basati su differenti ipotesi teoriche e indicazioni pratiche. Il primo libro di pianoforte, qualunque sia il suo valore, stabilisce il primo contatto con lo strumento, lasciando una forte impressione e tracciando il profilo delle competenze inizialmente sviluppate. Una più profonda comprensione di questi testi può mostrare quali sono le capacità e le strategie di apprendimento proposte dai metodi adottati e conseguentemente dagli insegnanti. L'obiettivo di questo studio è quello di identificare quali sono le scelte metodologiche più rappresentative nella didattica pianistica italiana degli ultimi 50 anni. I risultati sono basati sui dati raccolti dai questionari somministrati a 200 pianisti, di età compresa tra 20 e 60, ai quali è stato chiesto, insieme a informazioni generiche, quale fosse stato il loro primo libro pianoforte, quali aspetti positivi o negativi ricordavano maggiormente e quali ritenevano essere più utili. I dati suggeriscono una preferenza rilevante di cinque metodi, che sono stati analizzati secondo una serie di criteri metodologici e analitici. I risultati indicano un panorama metodologico concentrato principalmente sul rapporto iniziale con lo strumento e sulla lettura musicale. Questo studio contribuisce alla riflessione su due aspetti metodologici: lo sviluppo di nuovi repertori e la proposta di metodologie in grado di rispettare il mondo emotivo e cognitivo dei principianti.

Influenza del trattamento della schizofrenia con neurolettici tipici o olanzapina sui costi sanitari e sugli outcomes lavorativi

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Angelomarco Barioglio

2005-06-01

Full Text Available The aim of the present study was to evaluate the global treatment cost of schizophrenia with olanzapine or typical neuroleptics, according to Local Health Care Unit point of view. This analysis was performed through naturalistic observation of a cohort of schizophrenic patients referring to Ascoli Piceno ASL Department of Mental Health during 4 years (2001-2004. During year 2003, investigators have identified a cohort of patients who were undergoing treatment switch from typical neuroleptics to olanzapine. These patients, after the switch, are observed prospectively for the next 2 years and retrospectively for the last 2 years of treament. This method allow us to collect data about 4 years of treatment: 2 years of typical neuroleptic treatment followed by 2 years of olanzapine treatment. The present work is presenting the analysis of the first 3 years of observation. The results of our analysis are demonstrating that olanzapine treatment, through a better patient-physician alliance and with rehabilitative activities, allow lower total medical costs for the treatment of schizophrenia than typical neuroleptics. The higher acquisition cost of olanzapine versus typical neuroleptics was compensated by less hospitalizations and territorial medical interventions. During olanzapine treatment patients followed more rehabilitative activities (+71,26%, p <0,0001 and increased their working activities (+39,13%, p< 0,001.

SOIL AND “CERRADO” TREES NUTRIENTS AND METALS IN ADJACENT SANITARY LANDFILL AREA

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Otacílio Antunes Santana

2008-09-01

Full Text Available This research verified the influence of a Sanitary Landfill located at the Jockey Club of the Brasilia City (JCB on the chemical contents in the tree species of “Cerrado”. Six 25 x 500 m blocks were established in the PNB to sample the soil and the trees to chemical analysis. Three blocks were established near the landfill area and three in the control area. Nitrogen, phosphorus, potassium, calcium, lead, chromium, copper and mercury were analyzed. The highest nutrients and metals concentrations in soil were sampled in landfill adjacent area. The significant, crescent and directly proportional relationship (R2 > 0.80; p < 0.001 were observed between the elements concentration analyzed in soil with the leaves tissues. Therefore, the studied landfill presences increased nutrients and metals concentrations in soil and leaf tissue, fact that did not occur in the control area.