WorldWideScience

Sample records for stronger antioxidant activity

  1. Antioxidant activity of Vitex agnus-castus L. extracts.

    Science.gov (United States)

    Sağlam, Hüsniye; Pabuçcuoğlu, Aysun; Kivçak, Bijen

    2007-11-01

    The ethanol, n-hexane and water extracts of Vitex agnus-castus L. leaves and fruits were screened for antioxidant activity. The antioxidant activity of plant extracts was determined by an improved assay based on the decolorization of the radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS.+). The water and ethanol extracts showed stronger antioxidant activity than the n-hexane extracts. Copyright (c) 2007 John Wiley & Sons, Ltd.

  2. Evaluation of the Antioxidant Activity of Syzygium cumini Leaves

    Directory of Open Access Journals (Sweden)

    Yi Ming Lin

    2008-10-01

    Full Text Available The antioxidant activity of Syzygium cumini leaf extracts was investigated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH free radical-scavenging and ferric-reducing antioxidant power (FRAP assays. The methanolic extract and its four water, ethyl acetate, chloroform, and n-hexane fractions were prepared and subjected to antioxidant evaluation. The results showed that the ethyl acetate fraction had stronger antioxidant activity than the other ones. HPLC data indicated that S. cumini leaf extracts contained phenolic compounds, such as ferulic acid and catechin, responsible for their antioxidant activity. A significant linear relationship between antioxidant potency, free radical-scavenging ability and the content of phenolic compounds of leaf extracts supported this observation.

  3. Antioxidant activity of Potentilla fruticosa

    NARCIS (Netherlands)

    Miliauskas, G.; Beek, van T.A.; Venskutonis, P.R.; Linssen, J.P.H.; Waard, de P.; Sudhölter, E.J.R.

    2004-01-01

    The molecular structures of the radical scavenging compounds present in extracts of Potentilla fruticosa blossoms were elucidated and the antioxidant activities of various extracts were determined. The activities of the different fractions were monitored by off-line and on-line RP-HPLC DPPH. and

  4. Antioxidative activity of Geranium macrorrhizum

    NARCIS (Netherlands)

    Miliauskas, G.; Beek, van T.A.; Venskutonis, P.R.; Linssen, J.P.H.; Waard, de P.

    2004-01-01

    The composition of radical-scavenging compounds from Geranium macrorrhizum leaves was analyzed and the antioxidative activities of various extracts was determined. Seven compounds, namely gallic acid, ellagic acid, 4-galloyl quinic acid, the flavonoid quercetin and three of its glycosides,

  5. for antimicrobial and antioxidant activities

    African Journals Online (AJOL)

    Yomi

    2012-01-31

    Jan 31, 2012 ... An acetone extract of Loxostylis alata was separated into six fractions based on polarity by a solvent- solvent fractionation procedure and the different fractions were screened for antimicrobial and antioxidant activities. The number of antimicrobial compounds in the carbon tetrachloride (CCl4), chloroform ...

  6. Antioxidant and antimicrobial activities of polyphenols from ...

    African Journals Online (AJOL)

    the medicinal plants were screened for their antioxidant and antimicrobial activities against pathogenic micro organisms (Staphylococcus aureus, Streptococcus pyogenes, Esherichia coli and Candida albicans). The medicinal plants displayed different polyphenols contents and antioxidant activities. In addition, varying ...

  7. Antioxidant and antibacterial activities of polyphenols from ...

    African Journals Online (AJOL)

    Polyphenols from four medicinal plants of Burkina Faso, Combretum micranthum, Khaya senegalensis, Pterocarpus erinaceus and Sida acuta, were screened for their antioxidant and antimicrobial activities against pathogenic bacteria. The medicinal plants displayed different polyphenols contents and antioxidant activities.

  8. Potent antioxidant dendrimers lacking pro-oxidant activity.

    Science.gov (United States)

    Lee, Choon Young; Sharma, Ajit; Uzarski, Rebecca L; Cheong, Jae Eun; Xu, Hao; Held, Rich A; Upadhaya, Samik K; Nelson, Julie L

    2011-04-15

    It is well known that antioxidants have protective effects against oxidative stress. Unfortunately, in the presence of transition metals, antioxidants, including polyphenols with potent antioxidant activities, may also exhibit pro-oxidant effects, which may irreversibly damage DNA. Therefore, antioxidants with strong free radical-scavenging abilities and devoid of pro-oxidant effects would be of immense biological importance. We report two antioxidant dendrimers with a surface rich in multiple phenolic hydroxyl groups, benzylic hydrogens, and electron-donating ring substituents that contribute to their potent free radical-quenching properties. To minimize their pro-oxidant effects, the dendrimers were designed with a metal-chelating tris(2-aminoethyl)amine (TREN) core. The dendritic antioxidants were prepared by attachment of six syringaldehyde or vanillin molecules to TREN by reductive amination. They exhibited potent radical-scavenging properties: 5 times stronger than quercetin and 15 times more potent than Trolox according to the 1,1-diphenyl-2-picrylhydrazyl assay. The antioxidant dendrimers also protected low-density lipoprotein, lysozyme, and DNA against 2,2'-azobis(2-amidinopropane) dihydrochloride-induced free radical damage. More importantly, unlike quercetin and Trolox, the two TREN antioxidant dendrimers did not damage DNA via their pro-oxidant effects when incubated with physiological amounts of copper ions. The dendrimers also showed no cytotoxicity toward Chinese hamster ovary cells. Copyright © 2010 Elsevier Inc. All rights reserved.

  9. Antioxidant and antimicrobial activities of polyphenols from ...

    African Journals Online (AJOL)

    USER

    2010-05-17

    May 17, 2010 ... The antioxidant properties and antimicrobial potential of three ethnomedicinal plants, (Momordica charanta, Senna alata and Nauclea lafifolia) extracted with acetone were investigated. Polyphenols from the medicinal plants were screened for their antioxidant and antimicrobial activities against pathogenic.

  10. Cosmeceutical values, antimicrobial activities and antioxidant ...

    African Journals Online (AJOL)

    Jane

    2011-10-24

    Oct 24, 2011 ... Key words: Antimicrobial, antioxidant, ethanol extract, water extract, cashew leaves, cosmeceutical. INTRODUCTION ... Anacardium occidentale (Cashew) leaves extract could ..... Antioxidant activity at different contact time for treatment of different concentration CLE in water with different amount of GAC.

  11. Stronger activation of SREBP-1a by nucleus-localized HBx

    International Nuclear Information System (INIS)

    Wu, Qi; Qiao, Ling; Yang, Jian; Zhou, Yan; Liu, Qiang

    2015-01-01

    We previously showed that hepatitis B virus (HBV) X protein activates the sterol regulatory element-binding protein-1a (SREBP-1a). Here we examined the role of nuclear localization of HBx in this process. In comparison to the wild-type and cytoplasmic HBx, nuclear HBx had stronger effects on SREBP-1a and fatty acid synthase transcription activation, intracellular lipid accumulation and cell proliferation. Furthermore, nuclear HBx could activate HBV enhancer I/X promoter and was more effective on up-regulating HBV mRNA level in the context of HBV replication than the wild-type HBx, while the cytoplasmic HBx had no effect. Our results demonstrate the functional significance of the nucleus-localized HBx in regulating host lipogenic pathway and HBV replication. - Highlights: • Nuclear HBx is more effective on activating SREBP-1a and FASN transcription. • Nuclear HBx is more effective on enhancing intracellular lipid accumulation. • Nuclear HBx is more effective on enhancing cell proliferation. • Nuclear HBx up-regulates HBV enhancer I/X promoter activity. • Nuclear HBx increases HBV mRNA level in the context of HBV replication

  12. Stronger activation of SREBP-1a by nucleus-localized HBx

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Qi [VIDO-InterVac, Veterinary Microbiology, University of Saskatchewan, Saskatoon (Canada); Qiao, Ling [VIDO-InterVac, University of Saskatchewan, Saskatoon, Saskatchewan (Canada); Yang, Jian [Drug Discovery Group, University of Saskatchewan, Saskatoon, Saskatchewan (Canada); Zhou, Yan [VIDO-InterVac, Veterinary Microbiology, Vaccinology and Immunotherapeutics, University of Saskatchewan, Saskatoon, Saskatchewan (Canada); Liu, Qiang, E-mail: qiang.liu@usask.ca [VIDO-InterVac, Veterinary Microbiology, Vaccinology and Immunotherapeutics, University of Saskatchewan, Saskatoon, Saskatchewan (Canada)

    2015-05-08

    We previously showed that hepatitis B virus (HBV) X protein activates the sterol regulatory element-binding protein-1a (SREBP-1a). Here we examined the role of nuclear localization of HBx in this process. In comparison to the wild-type and cytoplasmic HBx, nuclear HBx had stronger effects on SREBP-1a and fatty acid synthase transcription activation, intracellular lipid accumulation and cell proliferation. Furthermore, nuclear HBx could activate HBV enhancer I/X promoter and was more effective on up-regulating HBV mRNA level in the context of HBV replication than the wild-type HBx, while the cytoplasmic HBx had no effect. Our results demonstrate the functional significance of the nucleus-localized HBx in regulating host lipogenic pathway and HBV replication. - Highlights: • Nuclear HBx is more effective on activating SREBP-1a and FASN transcription. • Nuclear HBx is more effective on enhancing intracellular lipid accumulation. • Nuclear HBx is more effective on enhancing cell proliferation. • Nuclear HBx up-regulates HBV enhancer I/X promoter activity. • Nuclear HBx increases HBV mRNA level in the context of HBV replication.

  13. Antiradical, Chelating and Antioxidant Activities of Hydroxamic Acids and Hydroxyureas

    Directory of Open Access Journals (Sweden)

    Branka Zorc

    2011-07-01

    Full Text Available Reactive oxygen species, along with reactive nitrogen species, may play an important role in the pathogenesis and progress of many diseases, including cancer, diabetes and sickle cell disease. It has been postulated that hydroxyurea, one of the main treatments in sickle cell disease, achieves its activity partly also through its antioxidant properties. A series of hydroxyurea derivatives of L- and D-amino acid amides and cycloalkyl-N-aryl-hydroxamic acids was synthesized and investigated for their radical scavenging activity, chelating properties and antioxidant activity. All the compounds showed exceptional antiradical activities. For example, free radical scavenging activities of investigated hydroxyureas were higher than the activity of standard antioxidant, butylated hydroxyanisole (BHA. Moreover, most of the investigated hydroxamic acids were stronger Fe2+ ion chelators than quercetin. In addition, the investigated compounds, especially hydroxamic acids, were proven to be excellent antioxidants. They were as effective as BHA in inhibiting b-carotene-linoleic acid coupled oxidation. It is reasonable to assume that the antioxidant activity of the investigated compounds could contribute to their previously proven biological properties as cytostatic and antiviral agents.

  14. Physical activity, obesity and mortality: does pattern of physical activity have stronger epidemiological associations?

    DEFF Research Database (Denmark)

    Bauman, Adrian E.; Grunseit, Anne C.; Rangul, Vegar

    2017-01-01

    outcomes, compared to single time-point measure of PA. Methods: Data were the Danish MONICA (MONItoring Trends and Determinants in CArdiovascular Disease) study over three waves 1982-3 (time 1), 1987-8 (time 2) and 1993-4 (time 3). Associations between leisure time single time-point PA levels at time 1...... and time 3, and sport and active travel at times 1 and 2 with BMI, waist, hip circumference and mortality (death from coronary heart disease (CHD) and cardiovascular disease (CVD)) were compared to 'PA patterns' spanning multiple time points. PA pattern classified participants' PA as either 1) inactive...... or low PA at both time points; 2) moderate level PA at time 1 and high activity at time 3; or 3) a 'mixed PA pattern' indicating a varying levels of activity over time. Similarly, sport and active travel were also classified as indicating stable low, stable high and mixed patterns. Results...

  15. Stronger synergies

    CERN Document Server

    Antonella Del Rosso

    2012-01-01

    CERN was founded 58 years ago under the auspices of UNESCO. Since then, both organisations have grown to become world leaders in their respective fields. The links between the two have always existed but today they are even stronger, with new projects under way to develop a more efficient way of exchanging information and devise a common strategy on topics of mutual interest.   CERN and UNESCO are a perfect example of natural partners: their common field is science and education is one of the pillars on which both are built. Historically, they share a common heritage. Both UNESCO and CERN were born of the desire to use scientific cooperation to rebuild peace and security in the aftermath of the Second World War. "Recently, building on our common roots and in close collaboration with UNESCO, we have been developing more structured links to ensure the continuity of the actions taken over the years," says Maurizio Bona, who is in charge of CERN relations with international orga...

  16. Antioxidant Activity from Various Tomato Processing

    Directory of Open Access Journals (Sweden)

    Retno Sri Iswari

    2016-04-01

    Full Text Available Tomato is one of the high antioxidant potential vegetables. Nowadays, there are many techniques of tomato processings instead of fresh consumption, i.e. boiled, steamed, juiced and sauteed. Every treatment of cooking will influence the chemical compound inside the fruits and the body's nutrition intake. It is important to conduct the research on antioxidant compound especially lycopene, β-carotene, vitamin C, α-tocopherol, and its activity after processing. This research has been done using the experimental method. Tomatoes were cooked into six difference ways, and then it was extracted using the same procedure continued with antioxidant measurement. The research results showed that steaming had promoted the higher antioxidant numbers (lycopene. α-tocopherol, β-carotene and vitamin C and higher TCA and antioxidant activities in the tomatoes than other processings. It was indicated that steaming was the best way to enhance amount, capacity and activities of antioxidants of the tomatoes.

  17. Antidiabetic, Antihyperlipidemic and Antioxidant Activities of ...

    African Journals Online (AJOL)

    Those with blood glucose levels > 190 ± 8 mg/dl were administered the methanol leaf extract of. Buchanania lanzan ... blood glucose level, serum lipid profile, and significantly (p < 0.05) increased antioxidant activity as ... Conclusion: MEBL exhibits antidiabetic, antihyperlipidemic and antioxidant activities in diabetic rat and.

  18. Antioxidant activity of lichen Cetraria aculeata

    Directory of Open Access Journals (Sweden)

    Tomović Jovica

    2016-01-01

    Full Text Available The aim of the present study is to investigate the antioxidant properties of the lichen Cetraria aculeata. Antioxidant activity of the methanol and ethyl acetate extracts of lichen was tested by different methods including determination of total phenolics content, determination of total antioxidant capacity, DPPH free radical scavenging activity, inhibitory activity towards lipid peroxidation, ferrous ion chelating ability and hydroxyl radical scavenging activity. The extracts of the lichen C. aculeata showed significant antioxidant activity. The methanol extract showed higher values for total phenolics and total antioxidant capacity compared to the ethyl acetate extract, while the ethyl acetate extract demonstrated better results for DPPH radical scavenging, inhibitory activity towards lipid peroxidation, chelating ability and hydroxyl radical scavenging than the methanol extract. This is the first report of the antioxidant properties of Cetraria aculeata growing in Serbia. The results of antioxidant activity indicate the application of this lichen as source of natural antioxidants that could be used as a possible food supplement, in the pharmaceutical industry and in the treatment of various diseases.

  19. Antiradical and antioxidant activities of new bio-antioxidants.

    Science.gov (United States)

    Kancheva, V D; Saso, L; Angelova, S E; Foti, M C; Slavova-Kasakova, A; Daquino, C; Enchev, V; Firuzi, O; Nechev, J

    2012-02-01

    Antioxidants could be promising agents for management of oxidative stress-related diseases. New biologically active compounds, belonging to a rare class of natural lignans with antiangiogenic, antitumoral and DNA intercalating properties, have been recently synthesized. These compounds are benzo[kl]xanthene lignans (1,2) and dihydrobenzofuran neolignans (3,4). The radical scavenging and chain-breaking antioxidant activities of compounds 1-4 were studied by applying different methods: radical scavenging activity by DPPH rapid test, chain-breaking antioxidant activity and quantum chemical calculations. All studied compounds were found to be active as DPPH scavengers but reaction time with DPPH and compounds' concentrations influenced deeply the evaluation. The highest values of radical scavenging activity (%RSAmax) and largest rate constants for reaction with DPPH were obtained for compounds 2 and 3. Comparison of %RSAmax with that of standard antioxidants DL-α-tocopherol (TOH), caffeic acid (CA) and butylated hydroxyl toluene (BHT) give the following new order of %RSA max: TOH (61.1%) > CA (58.6%) > 3 (36.3%) > 2 (28.1%) > 4 (6.7%) > 1 (3.6%) = BHT (3.6%). Chain-breaking antioxidant activities of individual compounds (0.1-1.0 mM) and of their equimolar binary mixtures (0.1 mM) with TOH were determined from the kinetic curves of lipid autoxidation at 80 °C. On the basis of a comparable kinetic analysis with standard antioxidants a new order of the antioxidant efficiency (i.e., protection factor, PF) of compounds 1-4 were obtained: 2 (7.2) ≥ TOH (7.0) ≥ CA (6.7) > 1 (3.1) > 3 (2.2) > ferulic acid FA (1.5) > 4 (0.6); and of the antioxidant reactivity (i.e. inhibition degree, ID): 2 (44.0) > TOH (18.7) > CA (9.3) > 1 (8.4) > 3 (2.8) > FA (1.0) > 4 (0.9). The important role of the catecholic structure in these compounds, which is responsible for the high chain-breaking antioxidant activity, is discussed and a reaction

  20. Cellular antioxidant activity of common vegetables.

    Science.gov (United States)

    Song, Wei; Derito, Christopher M; Liu, M Keshu; He, Xiangjiu; Dong, Mei; Liu, Rui Hai

    2010-06-09

    The measurement of antioxidant activity using biologically relevant assays is important to screen fruits, vegetables, natural products, and dietary supplements for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies antioxidant activity using a cell culture model and was developed to meet the need for a more biologically representative method than the popular chemistry antioxidant capacity measures. The objective of the study was to determine the CAA, total phenolic contents, and oxygen radical absorbance capacity (ORAC) values of 27 vegetables commonly consumed in the United States. Beets, broccoli, and red pepper had the highest CAA values, whereas cucumber had the lowest. CAA values were significantly correlated to total phenolic content. Potatoes were found to be the largest contributors of vegetable phenolics and CAA to the American diet. Increased fruit and vegetable consumption is an effective strategy to increase antioxidant intake and decrease oxidative stress and may lead to reduced risk of developing chronic diseases, such as cancer and cardiovascular disease.

  1. Phytochemical Screening and Antioxidant Activities of Some ...

    African Journals Online (AJOL)

    Purpose: Oxidative stress has been shown to play an important role in the development of anaemia in malaria. Indeed, increase in total antioxidant status has been shown to be important in recovery from malaria. The antioxidant activities of four medicinal plants traditionally used in the treatment of malaria in southwestern ...

  2. Enteromorpha compressa Exhibits Potent Antioxidant Activity

    Directory of Open Access Journals (Sweden)

    Sanaa M. M. Shanab

    2011-01-01

    Full Text Available The green macroalgae, Enteromorpha compressa (Linnaeus Nees, Ulva lactuca, and E. linza, were seasonally collected from Abu Qir bay at Alexandria (Mediterranean Sea This work aimed to investigate the seasonal environmental conditions, controlling the green algal growth, predominance, or disappearance and determining antioxidant activity. The freshly collected selected alga (E. compressa was subjected to pigment analysis (chlorophyll and carotenoids essential oil and antioxidant enzyme determination (ascorbate oxidase and catalase. The air-dried ground alga was extracted with ethanol (crude extract then sequentially fractionated by organic solvents of increasing polarity (petroleum ether, chloroform, ethyl acetate, and water. Antioxidant activity of all extracts was assayed using different methods (total antioxidant, DPPH [2, 2 diphenyl-1-picrylhydrazyl], ABTS [2, 2 azino-bis ethylbenzthiazoline-6-sulfonic acid], and reducing power, and β-carotene linoleic acid bleaching methods. The results indicated that the antioxidant activity was concentration and time dependent. Ethyl acetate fraction demonstrated higher antioxidant activity against DPPH method (82.80% compared to the synthetic standard butylated hydroxyl toluene (BHT, 88.5%. However, the crude ethanolic extract, pet ether, chloroform fractions recorded lower to moderate antioxidant activities (49.0, 66.0, and 78.0%, resp.. Using chromatographic and spectroscopic analyses, an active compound was separated and identified from the promising ethyl acetate fraction.

  3. Antioxidant activity potential of gamma irradiated carrageenan

    International Nuclear Information System (INIS)

    Abad, Lucille V.; Relleve, Lorna S.; Racadio, Charles Darwin T.; Aranilla, Charito T.; De la Rosa, Alumanda M.

    2013-01-01

    The antioxidant capacity of irradiated κ-, ι-, λ-carrageenans were investigated using the hydroxyl radical scavenging assay, reducing power assay and DPPH radical scavenging capacity assay. The degree of oxidative inhibition increased with increasing concentration and dose. The type of carrageenan had also an influence on its antioxidant activity which followed the order of lambda< iota< kappa. Increase in oxidative property with radiation dose can be attributed mainly to the depolymerization of the carrageenans with corresponding increase in reducing sugar. The antioxidant properties of these carrageenan oligomers were lower than that of ascorbic acid and galactose sugar. - Highlights: • The antioxidant capacity of gamma irradiated κ-, ι-, λ-carrageenans increased with increasing concentration and dose. • The type of carrageenan had an influence on its antioxidant activity which followed the order of lambda< iota< kappa. • Increase in oxidative property with radiation dose can be attributed mainly to the depolymerization of the carrageenans with corresponding increase in reducing sugar

  4. Phenolic Profiles and Antioxidant Activity of Lotus Root Varieties

    Directory of Open Access Journals (Sweden)

    Yang Yi

    2016-06-01

    Full Text Available Lotus root attracts increasing attention mainly because of its phenolic compounds known as natural antioxidants. Its thirteen varieties were systematically analyzed on the content, distribution, composition and antioxidant activity of phenolic compounds for a better understanding of this aquatic vegetable. The respective mean contents of total phenolics in their flesh, peel and nodes were 1.81, 4.30 and 7.35 mg gallic acid equivalents (GAE/g fresh weight (FW, and those of total flavonoids were 3.35, 7.69 and 15.58 mg rutin equivalents/g FW. The phenolic composition determined by a high-performance liquid chromatography method varied significantly among varieties and parts. The phenolics of flesh were mainly composed of gallocatechin and catechin; those of peel and node were mainly composed of gallocatechin, gallic acid, catechin and epicatechin. The antioxidant activities of phenolic extracts in increasing order were flesh, peel and node; their mean concentrations for 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical were 46.00, 26.43 and 21.72 µg GAE/mL, and their mean values representing ferric reducing antioxidant power were 75.91, 87.66 and 100.43 µg Trolox equivalents/100 µg GAE, respectively. “Zoumayang”, “Baheou”, “No. 5 elian” and “Guixi Fuou” were the hierarchically clustered varieties with relatively higher phenolic content and stronger antioxidant activity as compared with the others. Especially, their nodes and peels are promising sources of antioxidants for human nutrition.

  5. Antioxidant and antimicrobial activities of Canarium schweinfurthii ...

    African Journals Online (AJOL)

    diphenylpicrylhydrazyl (DPPH) radical scavenging assay and the -carotene bleaching test. Butylated hydroxytoluene (BHT) was employed as a positive control. The essential oil showed antioxidant and DPPH radical scavenging activities, and it displayed ...

  6. Phytochemical analysis and antioxidant activities of Combretum ...

    African Journals Online (AJOL)

    Phytochemical analysis and antioxidant activities of Combretum molle and Pericopsis laxiflora. Kossi-Kuma Agbalevon Koevi, Vinsoun Millogo, Jean Baptiste Hzounda Fokou, Abdou Sarr, Georges Anicet Ouedraogo, Emmanuel Bassene ...

  7. Antioxidant activity of the microalga Spirulina maxima

    Directory of Open Access Journals (Sweden)

    M.S. Miranda

    1998-08-01

    Full Text Available Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients. Spirulina contains phenolic acids, tocopherols and ß-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated with and without the extract at 37oC. The IC50 (concentration which causes a 50% reduction of oxidation of the extract in this system was 0.18 mg/ml. The in vivo antioxidant capacity was evaluated in plasma and liver of animals receiving a daily dose of 5 mg for 2 and 7 weeks. Plasma antioxidant capacity was measured in brain homogenate incubated for 1 h at 37oC. The production of oxidized compounds in liver after 2 h of incubation at 37oC was measured in terms of thiobarbituric acid reactant substances (TBARS in control and experimental groups. Upon treatment, the antioxidant capacity of plasma was 71% for the experimental group and 54% for the control group. Data from liver spontaneous peroxidation studies were not significantly different between groups. The amounts of phenolic acids, a-tocopherol and ß-carotene were determined in Spirulina extracts. The results obtained indicate that Spirulina provides some antioxidant protection for both in vitro and in vivo systems.

  8. Antioxidant Activity of Hawaiian Marine Algae

    Directory of Open Access Journals (Sweden)

    Anthony D. Wright

    2012-02-01

    Full Text Available Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that could be a potential natural source of such antioxidants. The total antioxidant activity of organic extracts of 37 algal samples, comprising of 30 species of Hawaiian algae from 27 different genera was determined. The activity was determined by employing the FRAP (Ferric Reducing Antioxidant Power assays. Of the algae tested, the extract of Turbinaria ornata was found to be the most active. Bioassay-guided fractionation of this extract led to the isolation of a variety of different carotenoids as the active principles. The major bioactive antioxidant compound was identified as the carotenoid fucoxanthin. These results show, for the first time, that numerous Hawaiian algae exhibit significant antioxidant activity, a property that could lead to their application in one of many useful healthcare or related products as well as in chemoprevention of a variety of diseases including cancer.

  9. Antioxidant activity of Hawaiian marine algae.

    Science.gov (United States)

    Kelman, Dovi; Posner, Ellen Kromkowski; McDermid, Karla J; Tabandera, Nicole K; Wright, Patrick R; Wright, Anthony D

    2012-02-01

    Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that could be a potential natural source of such antioxidants. The total antioxidant activity of organic extracts of 37 algal samples, comprising of 30 species of Hawaiian algae from 27 different genera was determined. The activity was determined by employing the FRAP (Ferric Reducing Antioxidant Power) assays. Of the algae tested, the extract of Turbinaria ornata was found to be the most active. Bioassay-guided fractionation of this extract led to the isolation of a variety of different carotenoids as the active principles. The major bioactive antioxidant compound was identified as the carotenoid fucoxanthin. These results show, for the first time, that numerous Hawaiian algae exhibit significant antioxidant activity, a property that could lead to their application in one of many useful healthcare or related products as well as in chemoprevention of a variety of diseases including cancer.

  10. Compositional Analysis and Antioxidant Activity of Volatile ...

    African Journals Online (AJOL)

    camphor and phenolic compounds. There is no significant difference between the radical scavenging activities of the two essential oils. Keywords: S. verticillata, S. suffruticosa, essential oil, antioxidant activity, GC-MS, HPLC activity. Tropical Journal of Pharmaceutical Research is indexed by Science Citation Index ...

  11. [Comparison of antioxidant activity between two species of chamomiles produced in Xinjiang by TLC-bioautography].

    Science.gov (United States)

    Han, Song-Lin; Li, Xin-Xia; Mian, Qiang-Hui; Lan, Wei; Liu, Yan

    2013-01-01

    from the two species of chamomiles have significant difference in the antioxidant activity in TLC-bioautography. Specifically, the antioxidant activity of volatile oil extracts from chamomile is stronger than volatile oil extracts from Roman chamomile; the known antioxidant active components in volatile oil extracts from chamomile is en-yne-dicycloether, while all of the other three antioxidant active components as well as antioxidant active components in volatile oil extracts from Roman chamomile are unknown components and remain to be further determined. Considering the significant difference in the number of antioxidant active spots in volatile oil extracts from the two species of chamomiles, the result can be applied to distinguish the two species of chamomiles. The antioxidant activity determination result for flavone extracts from two species of chamomiles was consistent with the result of TLC-bioautography, showing that flavone extracts from chamomile and Roman chamomile are more antioxidant active, while that of Roman chamomile is stronger than chamomile. Flavone extracts from both of the two species of chamomiles contain apigenin and pigenin-7-glucoside, which are known, while all of the other five antioxidant active components contained in flavone extracts from chamomile and the other six antioxidant active components contained in flavone extracts from Roman chamomile are unknown and remain to be further identified. The method lays a foundation for further identification of antioxidant active components contained in chamomile.

  12. Preliminary phytochemical screening and antioxidant activity of ...

    African Journals Online (AJOL)

    GAE) and the reducing power of 0.071±0.03nm was obtained. The DPPH scavenging activity of the extract was found to be promising. There is no significant difference (p<0.05) in the antioxidant activity between the extract and those of the ...

  13. Antioxidant activity directed isolations form punica granatum

    International Nuclear Information System (INIS)

    Siddiqi, R.; Saeed, M.G.; Sayeed, S.A.

    2012-01-01

    The extracts derived from pomegranate juice following antioxidant activity directed isolation were screened for their antioxidant activity through their ability to scavenge 2,2- diphenyl-l-picrylhydrazyl (DPPH) radicals. Only fractions which exhibited >50 / 0 DPPH scavenging effect at each step of isolation were selected for further purification and their ability to reduce peroxide formation (peroxide value) in heated com oil. Phytochemical analysis of the pure compounds finally obtained, revealed the presence of pelargonidin-3- galactose (Pg-3-galactose), cyanidin-3-glucose (Cy-3-Glucose), gallic acid, quercetin and myricetin in the fractions exhibiting >50% DPPH scavenging potential. The order of antioxidant activity of these pure compounds by DPPH method was found to be gallic acid> quercetin> myricetin> Cy-3-galactose> Pg-3-Glucose while order with respect to reduction in peroxide value (PV) was the reverse of DPPH. (author)

  14. Stronger effects of Roundup than its active ingredient glyphosate in damselfly larvae.

    Science.gov (United States)

    Janssens, Lizanne; Stoks, Robby

    2017-12-01

    Pesticides are causing strong decreases in aquatic biodiversity at concentrations assumed safe by legislation. One reason for the failing risk assessment may be strong differences in the toxicity of the active ingredient of pesticides and their commercial formulations. Sublethal effects, especially those on behaviour, have been largely ignored in this context, yet can be equally important as lethal effects at the population and ecosystem levels. Here, we compared the toxicity of the herbicide Roundup and its active ingredient glyphosate on survival, but also on ecologically relevant sublethal traits (life history, behaviour and physiology) in damselfly larvae. Roundup was more toxic than glyphosate with negative effects on survival, behaviour and most of the physiological traits being present at lower concentrations (food intake, escape swimming speed) or even only present (survival, sugar and total energy content and muscle mass) following Roundup exposure. This confirms the toxicity of the surfactant POEA. Notably, also glyphosate was not harmless: a realistic concentration of 2mg/l resulted in reduced growth rate, escape swimming speed and fat content. Our results therefore indicate that the toxicity of Roundup cannot be fully attributed to its surfactant, thereby suggesting that also the new generation of glyphosate-based herbicides with other mixtures of surfactants likely will have adverse effects on non-target aquatic organisms. Ecotoxicological studies comparing the toxicity of active ingredients and their commercial formulations typically ignore behaviour while the here observed differential effects on behaviour likely will negatively impact damselfly populations. Our data highlight that risk assessment of pesticides ignoring sublethal effects may contribute to the negative effects of pesticides on aquatic biodiversity. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Chemical properties and antioxidant and antimicrobial activities of Slovenian propolis.

    Science.gov (United States)

    Mavri, Ana; Abramovič, Helena; Polak, Tomaž; Bertoncelj, Jasna; Jamnik, Polona; Smole Možina, Sonja; Jeršek, Barbara

    2012-08-01

    The chemical composition as well as the antioxidant and antimicrobial activities of two EtOH extracts of propolis (PEEs) from Slovenia were determined. EtOH was used as extracting solvent at 70 and 96%, providing the extracts PEE70 and PEE96, respectively. The extraction with 70% EtOH was more efficient than that with 96% EtOH, as the PEE70 was richer in total phenolic compounds than the PEE96. The Slovenian propolis was characterized by different phenolic acids and flavonoids. The PEE96 was slightly richer in three specific compounds, i.e., caffeic acid, ferulic acid, and luteolin, while all other substances detected showed higher contents in the PEE70. The PEE70 showed a stronger reducing power and ability to scavenge free radicals and metal ions than the PEE96. Both PEEs were in the main more effective against Gram-positive bacteria than against fungi and Gram-negative bacteria like Salmonella and Escherichia coli, with the exception of Campylobacter. The PEE96 decreased the intracellular oxidation in Saccharomyces cerevisiae in a dose-dependent manner. The antimicrobial activities and antioxidant properties were related to the total phenolic contents. The two PEEs have the potential for use as natural antimicrobial and antioxidant additives in foods. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich.

  16. Antioxidant activities of Eryngium caucasicum inflorescence.

    Science.gov (United States)

    Motallebi Riekandeh, S; Mazandarani, M; Ebrahimzadeh, M A; Zargari, M

    2016-03-01

    Medicinal plants are good sources for discovery of safe and new antioxidants. The aim of present study was to evaluate the efficiencies of three methods for evaluated for antioxidants from Eryngium caucasicum. Ultrasonically assisted extraction, Soxhlet extraction and percolation method were evaluated for the extraction of antioxidants from E. caucasicum inflorescence. Antioxidant activities of extracts were evaluated with four different in vitro tests. Soxhlet extract had higher total phenolic and flavonoid contents than other extracts. Soxhlet extract showed the highest activity in DPPH radical scavenging (IC50 = 83.1 ± 2.1 µg ml-1). Soxhlet extract showed the best activity in iron chelatory (IC50 = 272 ± 6.3 µg ml-1) followed by ultrasonic extract. Percolation extract showed higher NO radical scavenging (IC50 = 390 ± 11.4 µg ml-1) than other extracts. Extracts showed good reducing power (p > 0.05). The results obtained indicated that all three extraction methods especially Soxhlet method could effectively extract antioxidants from this plant.

  17. Antioxidant Enzyme Activities of some Brassica Species

    Directory of Open Access Journals (Sweden)

    Rodica SOARE

    2017-11-01

    Full Text Available This paper set out to comparatively study five species: white cabbage (Brassica oleracea L. var. capitata alba Alef., red cabbage (Brassica oleracea L. var. capitata f. rubra Alef., Kale (Brassica oleracea L. var. Acephala, cauliflower (Brassica oleracea var. botrytis and broccoli (Brassica oleracea var. cymosa in order to identify those with high enzymatic and antioxidant activities. The enzymatic activity of superoxide dismutase (SOD, catalase (CAT and soluble peroxidase (POX as well as the antioxidant activity against 2.2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS radical cation were determined. Total superoxide dismutase activity was measured spectrophotometrically based on inhibition in the photochemical reduction of nitroblue tetrazolium. Total soluble peroxidase was assayed by measuring the increase in A436 due to the guaiacol oxidation and the catalase activity was assayed through the colorimetric method. The capacity of extracts to scavenge the ABTS radical cation was assessed colorimetric using Trolox as a standard. The obtained results show that studied enzymatic activities and the antioxidant activity against ABTS vary depending on the analyzed species. So, among the studied Brassicaceae species, it emphasize red cabbage with the highest enzymatic activity (CAT 22.54 mM H2O2/min/g and POX 187.2 mM ΔA/1min/1g f.w. and kale with highest antioxidant activity, of 767 μmol TE/100g f.w. The results of this study recommendintroducing the studied varieties in diet due to the rich antioxidant properties.

  18. Antioxidant activity of puha (Sonchus oleraceus L.) as assessed by the cellular antioxidant activity (CAA) assay.

    Science.gov (United States)

    McDowell, Arlene; Thompson, Scott; Stark, Mirjam; Ou, Zong-Quan; Gould, Kevin S

    2011-12-01

    There is considerable interest in antioxidant dietary components that can be protective against degenerative diseases in humans. Puha (Sonchus oleraceus L.) is a rich source of polyphenols, and exhibits strong antioxidant activity as measured by the 2,2-diphenylpicrylhydrazyl (DPPH) assay. However, the potential of puha to protect against degenerative diseases requires that low molecular weight antioxidants (LMWA) are absorbed by, and active in, human cells. The cellular antioxidant activity (CAA) assay was used to investigate the antioxidant activity of puha leaf extracts. Preparation methods of freezing and freeze-drying reduced the total polyphenolic content compared with fresh puha, but did not affect the LMWA potential as determined by the DPPH assay. The IC(50) values were 0.012 ± 0.003 mg/mL and 0.010 ± 0.005 mg/mL for freeze-dried and fresh puha leaves, respectively. Using the CAA assay, it was shown that LMWAs from foliar extracts of puha were effectively absorbed into HepG2 cells, and exerted antioxidant activity at levels comparable to those of extracts from blueberry fruits, the much-touted antioxidant superfood. Methylene blue staining of HepG2 cells indicated that puha extracts were not cytotoxic at concentrations below 100 mg DW/mL. The data indicate the potential of puha as a nutraceutical supplement for human health. Copyright © 2011 John Wiley & Sons, Ltd.

  19. EXTRACTION AND ANTIOXIDANT ACTIVITIES OF TWO SPECIES ...

    African Journals Online (AJOL)

    2013-06-30

    Jun 30, 2013 ... ABSTRACT. The antioxidant of ethanolic extract of two species of Origanum and essential oil of plant. Origanum vulgare were investigated and also the total phenolic and flavonoid content measured. The radical scavenging activity was measured using the 2,2-diphenyl-1- picrylhydrazyl (DPPH) method.

  20. preliminary phytochemical screening and antioxidant activity

    African Journals Online (AJOL)

    DR. AMINU

    ABSTRACT. Antioxidant evaluation of Albizia chevalieri used in Northern Nigerian traditional medicine, was carried out using the free radical scavenging activity of the 1, 1-diphenyl-2-picrylhydrazyl radical. (DPPH), total phenolics content and reducing power assay on the methanol leave extract. The results of the DPPH ...

  1. Hypolipidemic, Hypoglycemic and Antioxidant Activities of Flower ...

    African Journals Online (AJOL)

    HP

    1Department of Chemistry, University of Lucknow, Lucknow-226007, 2Division of Biochemistry, Central Drug. Research ... models, viz, triton WR-1339 - induced hyperlipimea in rats as well as fructose-rich high fat diet. To ..... peroxidation in rat liver microsomes in vitro (Note: Change (%) in antioxidant activity is shown in.

  2. Polyphenols content, antioxidant and antimicrobial activities of ...

    African Journals Online (AJOL)

    25 wild plants were collected from the south of Tunisia. The dried aerial parts were extracted under a continuous reflux set-up in a Soxhlet extractor with hexane, ethyl acetate, methanol and water. The extracts were screened for total phenolic content, antioxidant and antimicrobial activities. Total phenolic contents were ...

  3. Antioxidative Activities of Natural Hydroxy-Bearing ...

    African Journals Online (AJOL)

    ischemia, Alzheimer, Parkinsonism and aging. [1]. Natural antioxidants are capable of trapping radicals to protect .... were analysed using SPSS statistic program. (version 13.0, SPSS Inc, Chicago, IL, USA). RESULTS .... active compound in anti-hemolysis of RBCs among them, so the influence of the concentrations on the ...

  4. CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF APIS ...

    African Journals Online (AJOL)

    CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF APIS. MELLIFERA BEE POLLEN FROM NORTHWEST ALGERIA. A. Rebiai* and T.Lanez. University of El Oued, VTRS Laboratory, P.O. Box 789, 39000, El Oued, Algeria. Received: 08 November 2012 / Accepted: 23 December 2012 / Published online: 31 ...

  5. Hypolipidemic, Hypoglycemic and Antioxidant Activities of Flower ...

    African Journals Online (AJOL)

    Purpose: To investigate the anti-dyslipidemic, anti-oxidant and anti-diabetic activities of the aqueous extract and solvent fractions of A. violacea flowers. Methods: The aqueous extract was fractionated into petroleum ether, ether, chloroform, chloroformmethanol (4:1) and chloroform-methanol (3:2) fractions. Lipid lowering ...

  6. Assessment of antioxidant activity of natural extracts

    Directory of Open Access Journals (Sweden)

    Michel Prost

    2010-01-01

    Full Text Available The aim of this study was investigate and compare the antioxidant activity of extracts from Labiatae and Oleaceae cell cultures and a natural Verbenaceae extract on pig whole blood. The antioxidant activitiy was assessed using the KRL biological test. Extract obtained from Ajuga reptans (Labiatae cell cultures and titrated at 50% of phenylpropanoids expressed as teupolioside (T, extract obtained from Siringa vulgaris (Oleaceae cell cultures and titrated at 50% of phenylpropanoids expressed as verbascoside (V, V and T 50/50 mixture and a natural Verbenaceae extract (NE titrated at 5% of phenylpropanoids, expressed as verbascoside, were tested for their ability to protects pig red blood cells from 2,2’azobis (2-amidinopropane dihydrochloride (AAPH induced hemolysis. The protective effect of the compounds was dose-dependent at concentrations of 0.1 to 1g/L. The active principle in the NE shows an antioxidant activity 2.27 times higher (P<0.01 than the cell culture extract V and 5.11 times higher (P<0.01 than the cell culture extract T. Taking into account the total phenylpropanoids content of cell cultures and natural extract, these results clearly indicate a greater antioxidant activity for the natural Verbaneceae extract.

  7. Carotenoids from Phaffia rhodozyma : Antioxidant activity and ...

    African Journals Online (AJOL)

    The main goal of this work was to establish the stability and antioxidant activity of the extracts obtained through different techniques for recovering carotenoids from Phaffia rhodozyma NRRL-Y 17268. The best conditions for extracting carotenoids through cell rupture with dimethylsulfoxide (DMSO) were found to be a ...

  8. Antioxidant activities of curcumin in allergic rhinitis.

    Science.gov (United States)

    Altıntoprak, Niyazi; Kar, Murat; Acar, Mustafa; Berkoz, Mehmet; Muluk, Nuray Bayar; Cingi, Cemal

    2016-11-01

    We investigated the antioxidant effects of curcumin in an experimental rat model of allergic rhinitis (AR). Female Wistar albino rats (n = 34) were divided randomly into four groups: healthy rats (control group, n = 8), AR with no treatment (AR + NoTr group, n = 10), AR with azelastine HCl treatment (AR + Aze group, n = 8), and AR with curcumin treatment (AR + Curc group, n = 8). On day 28, total blood IgE levels were measured. For measurement of antioxidant activity, the glutathione (GSH) level and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities were measured in both inferior turbinate tissue and serum. Malondialdehyde (MDA) levels were measured only in inferior turbinate tissue, and paraoxonase (PON) and arylesterase (ARE) activities were measured only in serum. Statistically significant differences were found for all antioxidant measurements (GSH levels and CAT, SOD, GSH-Px activities in the serum and tissue, MDA levels in the tissue, and PON and ARE activities in the serum) between the four groups. In the curcumin group, serum SOD, ARE, and PON and tissue GSH values were higher than the control group. Moreover, tissue GSH levels and serum GSH-Px activities in the curcumin group were higher than in the AR + NoTr group. In the azelastine group, except MDA, antioxidant measurement values were lower than in the other groups. Curcumin may help to increase antioxidant enzymes and decrease oxidative stress in allergic rhinitis. We recommend curcumin to decrease oxidative stress in allergic rhinitis.

  9. Antioxidant enzymes activities in obese Tunisian children

    Directory of Open Access Journals (Sweden)

    Sfar Sonia

    2013-01-01

    Full Text Available Abstract Background The oxidant stress, expected to increase in obese adults, has an important role in the pathogenesis of many diseases. It results when free radical formation is greatly increased or protective antioxidant mechanisms are compromised. The main objective of this study is to evaluate the antioxidant response to obesity-related stress in healthy children. Methods A hundred and six healthy children (54 obese and 52 controls, aged 6–12 years old, participated in this study. The collected data included anthropometric measures, blood pressure, fasting glucose, total cholesterol, triglycerides and enzymatic antioxidants (Superoxide dismutase: SOD, Catalase: CAT and Glutathione peroxidase: GPx. Results The first step antioxidant response, estimated by the SOD activity, was significantly higher in obese children compared with normal-weight controls (p  Conclusions The obesity-related increase of the oxidant stress can be observed even in the childhood period. In addition to the complications of an increased BMI, obesity itself can be considered as an independent risk factor of free radical production resulting in an increased antioxidant response.

  10. Antioxidant activity of Sphaerococcus coronopifolius associated bacteria

    Directory of Open Access Journals (Sweden)

    Nádia Fino

    2014-06-01

    Full Text Available Associated bacteria living on macroalgae surfaces are an interesting source of new secondary metabolites with biological activities. The aim of this study was the isolation and identification of epiphytic bacteria from the marine algae Sphaerococcus coronopifolius and the evaluation of the antioxidant activity of the bacteria extracts. The identification of epiphytic bacteria was determined by 16S rRNA gene sequencing. Bacteria extracts were obtained with methanol and dichloromethane (1:1 extraction. Antioxidant activity was evaluated by quantification of total phenolic content (TPC, 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging activity and oxygen radical absorbent capacity (ORAC. The extracts with higher antioxidant activity were tested on MCF-7 and HepG-2 cell lines in oxidative stress conditions induced by H2O2 at 0.2 mM and 0.5 mM, respectively. In total were isolated 21 Sphaerococcus coronopifolius associated bacteria and identified as Vibrio sp. (28.57%, Shewanella sp. (23.81%, Pseudoalteromonas sp. (19.05%, Bacillus sp. (9.52% and Halomonas sp. (9.52%. Two (9.52% of them presented less than 90% Basic Local Alignment Search Tool (BLAST match. The epiphytic bacteria with the most antioxidant potential evaluated by ORAC and DPPH methods were Sp2, Sp12, Sp23, Sp25 and Sp27. The strain Sp4 show high antioxidant activity in all antioxidant methods (ORAC, DPPH and TPC. In oxidative stress conditions on MCF-7 cell line, the extracts of bacteria (1mg.ml-1: 24hours Sp4 (16.15%, Sp25 (17.95% and Sp27 (10.65% prevented the cell death induced by H2O2. In the HepG-2 cell line was the extracts of Sp2 (9.01%, Sp4 (11.21%, Sp12 (7.20% and Sp23 (8.81% bacteria that high prevented the oxidative stress condition induced by H2O2. In conclusion, the Sphaerococcus coronopifolius associated bacteria can be an interesting and excellent source of marine natural compounds with antioxidant activity.

  11. Antioxidant activity of wheat and buckwheat flours

    Directory of Open Access Journals (Sweden)

    Sedej Ivana J.

    2010-01-01

    Full Text Available Antioxidative activities of wheat flours (type 500 and wholegrain and buckwheat flours (light and wholegrain were tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH·-scavenging activity, reducing power and chelating activity on Fe2+. Also, the content of the total phenolics of ethanolic extracts was estimated. Polyphenolics content (expressed as gallic acid equivalent, GAE in wheat flours varied between 37.1 and 137.2 μg GAE/g extract, while its content in buckwheat flour were at least four time higher and ranged between 476.3 and 618.9μg GAE/g extract. Ethanolic extracts of buckwheat flours exhibited higher antioxidant activities in all the assays, except for chelating activity. Regarding all the obtained results, it can be concluded that bakery products produced with buckwheat flour could be regarded as potential functional foods.

  12. In vitro and in vivo antioxidant activities of inulin

    OpenAIRE

    Shang, Hong-Mei; Zhou, Hai-Zhu; Yang, Jun-Yan; Li, Ran; Song, Hui; Wu, Hong-Xin

    2018-01-01

    This study was designed to investigate the in vitro and in vivo antioxidant activities of inulin. The in vitro assays demonstrated that the antioxidant activities of inulin, including the DPPH radical scavenging activity, ABTS scavenging activity and ferric reducing power, were weak and significantly lower than those of Vitamin C (P < 0.05). The influence of dietary supplementation with inulin on the antioxidant status of laying hens was evaluated with in vivo antioxidant assays. The results ...

  13. Antioxidant, Antibacterial activity and Brine shrimp toxicity test of some Mountainous Lichens from Nepal

    Directory of Open Access Journals (Sweden)

    Babita Paudel

    2012-01-01

    Full Text Available A total of twenty four lichen species belonging to six families were collected from mountainous region of Nepal. The methanol extracts of each species were tested for antimicrobial and antioxidant activitiesin vitro. It was found that extracts of twenty one lichen species were active againstB. subtilis and seven species were active againstS. aureus. Similarly, in DPPH assay, three speciesPeltigera sp.,Cladonia sp., andCanoparmelia sp. showed comparable activity with commercial standard, BHA. In ABTS+ assay, extracts ofParmoterma sp.,Ramalina sp.,Peltigera sp. andCladonia sp. showed stronger activity than ascorbic acid. The observed data after comparison with previously published reports indicated that the high altitude lichens contain stronger antioxidant and antibacterial constituents. Similarly, the methanol extracts ofHeterodermia sp. andRamalina sp. showed comparable toxicity effect with commercial standard berberine chloride indicating a potent source of anticancer drugs.

  14. Evaluation of antioxidant activity and polyphenolic contents of two ...

    African Journals Online (AJOL)

    Total phenolic content were determined by Folin-Ciocalteu test, and antioxidant activity measured using cyclic voltammetry. Correlations between antioxidant activity and total phenolic content were also examined. Within each cultivar, the total phenolic content and antioxidant activity were highest in the peels, followed by ...

  15. Mechanochemical synthesis and antioxidant activity

    Indian Academy of Sciences (India)

    Curcumin, the active ingredient of turmeric, is a diaryl- heptanoid natural product that is endowed with much bioactivity;1 3 yet it has limited applications as a drug due to its low bioavailability.4 Structural modifi- cation of curcumin has been explored much as a strategy to circumvent this inadequacy. Among such structurally.

  16. Antioxidative Activity of Tobacco Leaf Protein Hydrolysates

    Directory of Open Access Journals (Sweden)

    Guohua Rao

    2007-01-01

    Full Text Available Discarded tobacco leaf protein hydrolysate (DTLPH was prepared by enzymatic hydrolysis using papain and then separated using ultrafiltration (UF membranes with molecular mass cut-off (MMCO of 10, 5, 3 and 1 kDa. Four permeate fractions including 10-K, 5-K, 3-K and 1-K (the permeate fractions from 10, 5, 3 and 1 kDa hydrolysate fractions were obtained. The 5-K hydrolysate fraction had high oxidation inhibilitory ratio (42.62 %, which was about twofold higher than the original hydrolysate and as high as that of vitamin E (α-tocopherol. The fractionated hydrolysates were superior to the original hydrolysate in the antioxidative activity tested. Moreover, these separated hydrolysates showed the enhanced functional property. The amino acid composition of 5-K hydrolysate was analyzed and the results show that the high antioxidative activity of 5-K hydrolysate was derived from high content of histidine, methionine, cystine and tryptophan.

  17. Chemical Composition, antioxidant activity, functional properties and ...

    African Journals Online (AJOL)

    Chemical Composition, antioxidant activity, functional properties and inhibitory action of unripe plantain ( M. Paradisiacae ) flour. ... of dry matter (48.00 ± 3.96%) and starch (31.10 ± 0.44%) but was low in phenol (1.42 ± 0.03%), protein (3.15 ± 0.042%), ash (5.50 ± 0.42%) and total soluble sugar (0.64 ± 0.001%) (p < 0.05).

  18. In Vitro Analysis of Antioxidant Activities of Oxalis Corniculata Linn ...

    African Journals Online (AJOL)

    In Vitro Analysis of Antioxidant Activities of Oxalis Corniculata Linn. Fractions in Various Solvents. D Ahmed, S Zara, H Baig. Abstract. As part of our search for natural antioxidants, this work presents an evaluation of antioxidant activities of methanolic extract of Oxalis corniculata and its sub-fractions in hexane, chloroform, ...

  19. Total phenolic, condensed tannin and antioxidant activity of four ...

    African Journals Online (AJOL)

    Different species of functional agricultural crops may vary in antioxidant capacities. In this study, the antioxidant activities of methanol extracts from four species of Carya genus were compared by various antioxidant assays, including the reducing power, 1,1-diphenyl-2-pycrylhydrazyl (DPPH) radical scavenging activity and ...

  20. Antioxidant Activity, Antitumor Effect, and Antiaging Property of Proanthocyanidins Extracted from Kunlun Chrysanthemum Flowers

    Directory of Open Access Journals (Sweden)

    Siqun Jing

    2015-01-01

    Full Text Available The objective of the present study was to evaluate the antioxidant activity, antitumor effect, and antiaging property of proanthocyanidins from Kunlun Chrysanthemum flowers (PKCF grown in Xinjiang. In vitro antioxidant experiments results showed that the total antioxidant activity and the scavenging capacity of hydroxyl radicals (•OH and 1,1-diphenyl-2-picrylhydrazyl (DPPH• radicals increased in a concentration-dependent manner and were stronger than those of vitamin C. To investigate the antioxidant activity of PKCF in vivo, we used serum, liver, and kidney from mouse for the measurement of superoxide dismutase (SOD, malondialdehyde (MDA, and total antioxidant capacity (T-AOC. Results indicated that PKCF had antioxidative effect in vivo which significantly improved the activity of SOD and T-AOC and decreased MDA content. To investigate the antitumor activity of PKCF, we used H22 cells, HeLa cells, and Eca-109 cells with Vero cells as control. Inhibition ratio and IC50 values were measured by 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assay; PKCF showed great inhibitory activity on H22 cells and HeLa cells. We also used fruit flies as a model for analyzing the anti-aging property of PKCF. Results showed that PKCF has antiaging effect on Drosophila. Results of the present study demonstrated that PKCF could be a promising agent that may find applications in health care, medicine, and cosmetics.

  1. In vitro and in vivo antioxidant activities of inulin.

    Science.gov (United States)

    Shang, Hong-Mei; Zhou, Hai-Zhu; Yang, Jun-Yan; Li, Ran; Song, Hui; Wu, Hong-Xin

    2018-01-01

    This study was designed to investigate the in vitro and in vivo antioxidant activities of inulin. The in vitro assays demonstrated that the antioxidant activities of inulin, including the DPPH radical scavenging activity, ABTS scavenging activity and ferric reducing power, were weak and significantly lower than those of Vitamin C (P inulin on the antioxidant status of laying hens was evaluated with in vivo antioxidant assays. The results indicated that inulin supplementation quadratically improved the egg production rate of the laying hens (P inulin levels increased (P inulin levels increased (P inulin has the potential to improve the antioxidant status of laying hens.

  2. Antioxidant activity of amino acids in soybean oil at frying temperature: Structural effects and synergism with tocopherols.

    Science.gov (United States)

    Hwang, Hong-Sik; Winkler-Moser, Jill K

    2017-04-15

    The purpose of this study was to evaluate amino acids as natural antioxidants for frying. Twenty amino acids were added to soybean oil heated to 180°C, and the effects of amino acid structure on the antioxidant activity were investigated. Amino acids containing a thiol, a thioether, or an extra amine group such as arginine, cysteine, lysine, methionine, and tryptophan had the strongest antioxidant activities. At 5.5mM, these amino acids had stronger antioxidant activities than 0.02% (1.1mM) tert-butylhydroquinone (TBHQ). A functional group such as an amide, carboxylic acid, imidazole, or phenol appeared to negatively affect amino acid antioxidant activity. Synergism between amino acids and tocopherols was demonstrated, and we found that this synergistic interaction may be mostly responsible for the antioxidant activity that was observed. In a frying study with potato cubes, 5.5mM l-methionine had significantly stronger antioxidant activity than 0.02% TBHQ. Published by Elsevier Ltd.

  3. [Antioxidant activity of cationic whey protein isolate].

    Science.gov (United States)

    titova, M E; Komolov, S A; Tikhomirova, N A

    2012-01-01

    The process of lipid peroxidation (LPO) in biological membranes of cells is carried out by free radical mechanism, a feature of which is the interaction of radicals with other molecules. In this work we investigated the antioxidant activity of cationic whey protein isolate, obtained by the cation-exchange chromatography on KM-cellulose from raw cow's milk, in vitro and in vivo. In biological liquids, which are milk, blood serum, fetal fluids, contains a complex of biologically active substances with a unique multifunctional properties, and which are carrying out a protective, antimicrobial, regenerating, antioxidant, immunomodulatory, regulatory and others functions. Contents of the isolate were determined electrophoretically and by its biological activity. Cationic whey protein isolate included lactoperoxidase, lactoferrin, pancreatic RNase, lysozyme and angeogenin. The given isolate significantly has an antioxidant effect in model experimental systems in vitro and therefore may be considered as a factor that can adjust the intensity of lipid oxidation. In model solutions products of lipid oxidation were obtained by oxidation of phosphatidylcholine by hydrogen peroxide in the presence of a source of iron. The composition of the reaction mixture: 0,4 mM H2O2; 50 mcM of hemin; 2 mg/ml L-alpha-phosphatidylcholine from soybean (Sigma, German). Lipid peroxidation products were formed during the incubation of the reaction mixture for two hours at 37 degrees C. In our studies rats in the adaptation period immediately after isolation from the nest obtained from food given orally native cationic whey protein isolate at the concentration three times higher than in fresh cow's milk. On the manifestation of the antioxidant activity of cationic whey protein isolate in vivo evidence decrease of lipid peroxidation products concentration in the blood of rats from the experimental group receipt whey protein isolate in dos 0,6 mg/g for more than 20% (pisolate has an antioxidant

  4. In vitro and in vivo antioxidant activities of inulin.

    Directory of Open Access Journals (Sweden)

    Hong-Mei Shang

    Full Text Available This study was designed to investigate the in vitro and in vivo antioxidant activities of inulin. The in vitro assays demonstrated that the antioxidant activities of inulin, including the DPPH radical scavenging activity, ABTS scavenging activity and ferric reducing power, were weak and significantly lower than those of Vitamin C (P < 0.05. The influence of dietary supplementation with inulin on the antioxidant status of laying hens was evaluated with in vivo antioxidant assays. The results indicated that inulin supplementation quadratically improved the egg production rate of the laying hens (P < 0.01. The antioxidant enzyme activities in the serum, including SOD, CAT, and GSH-Px, and the total antioxidant capacity increased quadratically as inulin levels increased (P < 0.001. The levels of MDA in the serum decreased quadratically as inulin levels increased (P < 0.001. These findings suggest that inulin has the potential to improve the antioxidant status of laying hens.

  5. Antioxidant and Anticancer activities of yeast grown on commercial ...

    African Journals Online (AJOL)

    Antioxidant and Anticancer activities of yeast grown on commercial media. Amal S. Shahat. Abstract. The use of Synthetic antioxidants is being widely restricted because of their reported toxic and carcinogenic effects. Thus, now there is considerable interest being focused towards finding antioxidants from natural sources ...

  6. Isoflavone content and antioxidant activity of Thai fermented ...

    African Journals Online (AJOL)

    USER

    2010-06-28

    Jun 28, 2010 ... Value in table expressed as mean ± SD. Means in each column with different letters are significantly different (p < 0.05). 1 TEAC: Trolox equivalent antioxidant capacity; 2 FRAP: Ferric reducing antioxidant power. Table 3. Weight variation, disintegration, antioxidant activity, and isoflavone contents of hard ...

  7. Antioxidant activities in extracts of selected indigenous vegetables ...

    African Journals Online (AJOL)

    Antioxidant activities in extracts of selected indigenous vegetables from Kenya and Malawi. ... intake of these vegetables species in sufficient concentrations should thus be recommended to enhance an optimal antioxidant capacity in the body. Keywords: Antioxidants, free radicals, health benefits, indigenous vegetables

  8. Preparation of the different derivatives of the low-molecular-weight porphyran from Porphyra haitanensis and their antioxidant activities in vitro.

    Science.gov (United States)

    Zhang, Zhongshan; Zhang, Quanbin; Wang, Jing; Zhang, Hong; Niu, Xizhen; Li, Pengcheng

    2009-07-01

    Porphyran extracted from Porphyra haitanensis is a sulfated polysaccharide, which possesses excellent antioxidant activities. In this study, we prepared one low-molecular-weight porphyran and its sulfated, acetylated, phosphorylated and benzoylated derivatives. Their antioxidant activities were investigated including scavenging effect of superoxide, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals. The results of chemical analysis and FT-IR spectrums showed the modification was successful. And in addition, we found that certain derivative exhibited stronger antioxidant activity than low-molecular-weight porphyran. The benzoylated derivative showed the most excellent antioxidant activity in three assays, so this derivative needs to be attended to.

  9. Scuba diving activates vascular antioxidant system.

    Science.gov (United States)

    Sureda, A; Batle, J M; Ferrer, M D; Mestre-Alfaro, A; Tur, J A; Pons, A

    2012-07-01

    The aim was to study the effects of scuba diving immersion on plasma antioxidant defenses, nitric oxide production, endothelin-1 and vascular endothelial growth factor levels. 9 male divers performed an immersion at 50 m depth for a total time of 35 min. Blood samples were obtained before diving at rest, immediately after diving, and 3 h after the diving session. Leukocyte counts, plasma 8oxoHG, malondialdehyde and nitrite levels significantly increased after recovery. Activities of lactate dehydrogenase, creatine kinase, catalase and superoxide significantly increased immediately after diving and these activities remained high after recovery. Plasma myeloperoxidase activity and protein levels and extracellular superoxide dismutase protein levels increased after 3 h. Endothelin-1 concentration significantly decreased after diving and after recovery. Vascular endothelial growth factor concentration significantly increased after diving when compared to pre-diving values, returning to initial values after recovery. Scuba diving at great depth activated the plasma antioxidant system against the oxidative stress induced by elevated pO₂ oxygen associated with hyperbaria. The decrease in endothelin-1 levels and the increase in nitric oxide synthesis could be factors that contribute to post-diving vasodilation. Diving increases vascular endothelial growth factor plasma levels which can contribute to the stimulation of tissue resistance to diving-derived oxidative damage. © Georg Thieme Verlag KG Stuttgart · New York.

  10. Antioxidant and antimicrobial activities of helinus lanceolatus

    International Nuclear Information System (INIS)

    Ajaib, M.

    2015-01-01

    The extracts of petroleum ether, chloroform, methanol and aqueous of Helinus lanceolatus were tested for their antioxidant potential, antibacterial and antifungal activities. The results revealed that the methanolic extract showed the highest zone of inhibition 50 ± 1.15 mm against Escherichia coli and aqueous extract shows excellent inhibition for fungi Aspergillus niger 17 ± 2.6 mm. The water extract showed highest DPPH radical scavenging activity i.e. 91.8 - 0.0.09% at a concentration of 500 mu g/ml with IC50 value 12.29 ± 0.59 micro g/ml relative to butylated hydroxyltoluene (BHT) having IC50 value 12.52 ± 0.89 micro g/ml. Chloroform extract showed highest antioxidant activity 0.840 ± 0.13 micro g/ml relative to standard 0.891 ± 0.13 micro g/ml while the highest FRAP value i.e. 90.66 ± 4.54 TE micro g/ml was shown by petroleum ether fraction. Methanolic extract also showed good value of inhibition of lipid peroxidation, i.e. 59.11 ± 0.12%. (author)

  11. Antioxidant activities of Barringtonia racemosa leaves

    Directory of Open Access Journals (Sweden)

    Nurul Syafizan, R.

    2017-12-01

    Full Text Available Proximate analysis of Barringtonia racemosa (putat leaves was carried out and total phenolic content (TPC was measured using total phenolic assay, also known as the Folin Ciocalteu (FC assay. Antioxidant activities of the methanolic and aqueous extracts were evaluated by ferric reducing antioxidant power (FRAP assay; the free radical scavenging capacity was determined by 1,1-diphenyl-1-picrylhydrazyl radical scavenging capacity (DPPH assay. Proximate analysis showed that the leaves contained 70.52% moisture, 0.96% ash, 2.54% protein, 0.26% fat, 1.38% crude fiber, and 25.71% carbohydrates. The TPC amount exhibited by the methanolic extract was 1585.20±25.54 mg GAE/100 g sample, which was higher compared with the aqueous plant extract with 1314.12±66.19 mg GAE/100 g (p<0.05. Significant (p<0.05 difference in terms of the flavonoid content was observed between the extracts obtained by methanolic and aqueous extraction. Total condensed tannin content showed significant (p<0.05 difference between the methanolic and the aqueous extracts, which were 33.11±0.61 mg CE/100 g and 13.38±0.87 mg CE/100 g, respectively. Methanolic extract of Barringtonia racemosa leaves showed a higher DPPH scavenging activity than the aqueous extract. FRAP value for the methanolic extract of the sample was higher than the aqueous extract, with a significant difference (p<0.05. Results of this study will provide a platform for the future paradigm of these potential natural sources of antioxidants in food and nutraceutical applications.

  12. Degradation and the antioxidant activity of polysaccharide from Enteromorpha linza.

    Science.gov (United States)

    Zhang, Zhongshan; Wang, Xiaomei; Mo, Xiaofang; Qi, Huimin

    2013-02-15

    Polysaccharide extracted from Enteromorpha linza possesses excellent antioxidant activities, but its molecular weight was greatly high which influences the activity. In this study, the combination of ascorbic acid and H(2)O(2) was used as degradation reagents in order to obtain the lower molecular weight product. The results suggested that the most effective molar ratio of the two reagents was 1. Three degraded polysaccharides were selected to evaluate their antioxidant activities in vitro and characterized the relationship between antioxidant activity and chemical characteristics. It was found that the degraded sample with lower molecular weight possessed the higher antioxidant activities. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. Antioxidant and Anti-Fatigue Activities of Flavonoids from Puerariae ...

    African Journals Online (AJOL)

    This study evaluated the antioxidant and anti-fatigue activities of flavonoids from Puerariae radix (FPR). In vitro antioxidant activities of FPR were investigated through hydroxyl and superoxide radical scavenging activities. In vivo anti-fatigue activity of FPR was investigated through loaded swimming exercise of mice. Results ...

  14. A comparative study on the antioxidant activity of fringe tree ...

    African Journals Online (AJOL)

    Administrator

    2007-02-19

    Feb 19, 2007 ... reducing activity, hydrogen peroxide (H2O2) scavenging and ferrous metal chelating activities were for used antioxidant evaluation of MEFT and EEFT. The both extracts neutralized the activities of radicals and inhibited the peroxidation reactions of linoleic acid emulsion. Total antioxidant activity was.

  15. Antioxidant activity of medicinal plant polysaccharides.

    Science.gov (United States)

    Kardosová, A; Machová, E

    2006-07-01

    Eleven polysaccharides have been isolated from the leaves of Arctium lappa var. herkules, Aloe barbadensis, Althaea officinalis var. robusta, Plantago lanceolata var. libor, aerial parts and roots of Rudbeckia fulgida var. sullivantii, stems of Mahonia aquifolium, and peach-tree (Prunus persica) gum exudates. The polysaccharides were investigated for their ability to inhibit peroxidation of soyabean lecithin liposomes by OH radicals. The highest inhibition was found with glucuronoxylans of A. officinalis var. robusta and P. lanceolata var. libor, aerial parts. Their antioxidant activity accounted for approximately 69% of the activity of the reference compound alpha-tocopherol. The activity of eight polysaccharides ranged from 20 to 45%, while the fructofuranan from P. lanceolata var. libor roots was practically inactive.

  16. Antioxidant and antimicrobial activities of Shorea kunstleri

    Directory of Open Access Journals (Sweden)

    Siti Suria Daud

    2014-08-01

    Full Text Available Objective: To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri (S. kunstleri together with analysis of phytochemical and total phenolic contents. Methods: Extraction was conducted with different solvent polarity of n-hexane, dichloromethane (DCM and methanol by using Soxhlet extraction. Total phenolic content was determined using Folin-Ciocalteu method. Free radical scavenging activity and inhibition of lipid peroxidation were evaluated with DPPH radical scavenging and ferric thiocyanate assays, respectively. Antimicrobial activities were performed using disc diffusion method, minimum inhibition concentration (MIC, minimum bactericidal concentration (MBC, and minimum fungicidal concentration. Results: S. kunstleri stembark extracts revealed presence of steroids, terpenoids, saponins, flavonoids, and phenolic compounds. Methanol extract exhibited the highest total phenolic content and free radical scavenging activity resulting in phenolic content of (8.340±0.003 g GAE/100 g of extract and (95.90±1.07% DPPH inhibition (IC50 value of 18.6 µg/mL, respectively. Ferric thiocyanate assay of n-hexane, DCM, and methanol extracts indicated lipid peroxidation inhibitory activity of (74.20±0.35%, (74.00±0.10%, and (72.80±0.27%, respectively. In antimicrobial and antifungal tests, methanol extract showed inhibition against Staphylococcus aureus (S. aureus, Candida albicans, and Candida tropicalis with inhibition zones of 10-12, 18-22, and 18-19 mm, respectively. The MIC test of methanol extract showed highest inhibition against Candida albicans and S. aureus (0.04 and 0.08 mg/mL, respectively while DCM extract exhibited the highest activity towards Candida tropicalis (MIC value of 0.63 mg/mL. Taken together, MBC test of methanol extract strongly demonstrated bactericidal effect against S. aureus with MBC value of 0.08 mg/mL. Conclusions: The study demonstrated that stembark extracts of S. kunstleri possessed antioxidant

  17. Carbon sink activity is stronger under grazing than under mowing: results from a paired eddy flux towers experiment

    Science.gov (United States)

    Pintér, Krisztina; Balogh, János; Koncz, Péter; Hidy, Dóra; Cserhalmi, Dóra; Papp, Marianna; Fóti, Szilvia; Nagy, Zoltán

    2014-05-01

    Effect of grazing vs. mowing on carbon balance of a grassland was investigated by a paired eddy towers (one of them measuring the grazed, the another the mowed treatment) experiment at the Bugacpuszta sandy grassland site (HU-Bug, 46.69° N, 19.6° E, 114m asl, 10.4 ° C annual mean temperature, 562 mm annual precipitation sum) located in the Hungarian Plain. Eddy covariance measurements started in July, 2002. The area of the mowed treatment is 1 ha, it is located within the grazed treatment (500 ha). Electric fence was set up around the selected area in spring of 2011. Study years include 2011, 2012 and 2013. The pasture is managed extensively (average grazing pressure of 0.5 cattle per hectare), the cattle herd regularly took several kilometres during a grazing day. Annual net ecosystem exchange (NEE) of the grassland is strongly limited by precipitation, there were 2 source years within the 11 years (2003-2013) of measurements, during which the average annual balance was -109 gCm-2year-1 with standard deviation of 106 gCm-2year-1. Carbon sink activity of the grassland was stronger in the grazed treatment than in the mowed treatment during the three year study period (paired t-test, P=0.058). In the grazed treatment the average sink strength was -142.8 ±40 gCm-2year-1, while in the mowed treatment the average sink strength was -61.5 ±46.5 gCm-2year-1. Differences of carbon balances between the treatments were positively correlated to the annual sum of evapotranspiration (ET), while ETs of the treatments were almost identical (differences within a 10mm year-1 range) in each study year. Water use efficiency in the mowed treatment was 44% of that in the grazed treatment (P=0.045) as a result of the differences in sink capacity. The higher sensitivity to drought by the mowed treatment manifested in decreased sink capacity during summer and in decreased regeneration capacity during autumn rains as shown by the cumulative NEE in the different years. Minor but

  18. Antioxident activity of the mangrove endophytic fungus (Trichoderma sp.

    Directory of Open Access Journals (Sweden)

    Saravanakumar Kandasamy

    2014-07-01

    Full Text Available Objective: To test antioxidant property of the endophytic Trichoderma species isolated from the leaves of 12 mangroves of Andaman Nicobar Islands. Methods: Eight strains of Trichoderma species were found predominant and their crude extracts were assessed for antioxidant activity by using seven assays. Results: Total antioxidant activity varied with the strains and it was maximum in Trichoderma EMFCAS8 and other strains also showed considerable activity. Conclusions: This work concluded that mangroves are rich in endophytic Trichoderma species with potential for antioxidant activity.

  19. Antioxidant and antibacterial activities of different plants extracts ...

    African Journals Online (AJOL)

    Antioxidant and antibacterial activities of different plants extracts against Staphylococcus aureus isolated from soccer player's shoes and knowledge and applications about foot hygiene of the soccer players.

  20. Tetrahydrofolate and 5-methyltetrahydrofolate are folates with high antioxidant activity. Identification of the antioxidant pharmacophore.

    NARCIS (Netherlands)

    Rezk, BM; Haenen, GR; Vijgh, van der W.J.F.

    2003-01-01

    The presumed protective effect of folic acid on the pathogenesis of cardiovascular, hematological and neurological diseases and cancer has been associated with the antioxidant activity of folic acid. Peroxynitrite (PON) scavenging activity and inhibition of lipid peroxidation (LPO) of the

  1. Anti-Inflammatory and Antioxidant Activities of Methanol Extracts and ...

    African Journals Online (AJOL)

    Anti-Inflammatory and Antioxidant Activities of Methanol Extracts and Alkaloid Fractions of four Mexican Medicinal Plants of Solanaceae. ... equivalents/g of extract), the best antioxidant activity (94.80% inhibition of DPPH and 97.57% of ABTS) and the highest anti-inflammatory activity (81.93% inhibition of the inflammation).

  2. Microwave-assisted extraction of Nigella sativa L. essential oil and evaluation of its antioxidant activity.

    Science.gov (United States)

    Abedi, Abdol-Samad; Rismanchi, Marjan; Shahdoostkhany, Mehrnoush; Mohammadi, Abdorreza; Mortazavian, Amir Mohammad

    2017-11-01

    It has been previously reported that the essential oil of Nigella sativa L. seeds and its major active component, thymoquinone (TQ), possess a broad variety of biological activities and therapeutic properties. In this work, microwave-assisted extraction (MAE) of the essential oil from Nigella sativa L. seeds and its antioxidant activity were studied. Response surface methodology based on central composite design was used to evaluate the effects of extraction time, irradiation power and moisture content on extraction yield and TQ content. Optimal parameters obtained by CCD and RSM were extraction time 30 min, irradiation power 450 W, and moisture content 50%. The extraction yield and TQ content of the essential oil were 0.33 and 20% under the optimum conditions, respectively. In contrast, extraction yield and TQ amount of oil obtained by hydrodistillation (HD) were 0.23 and 3.71%, respectively. The main constituents of the essential oil extracted by MAE and HD were p -cymene, TQ, α-thujene and longifolene, comprising more than 60% of total peak area. The antioxidant capacity of essential oils extracted by different methods were evaluated using 2,2-diphenyl-1-picrylhydrazyl and Ferric reducing antioxidant power assays, and compared with traditional antioxidants. The results showed that MAE method was a viable alternative to HD for the essential oil extraction from N. sativa seeds due to the excellent extraction efficiency, higher thymoquinone content, and stronger antioxidant activity.

  3. In vitro antioxidant activity of Rubus ellipticus fruits

    OpenAIRE

    Sharma, Uma Shankar; Kumar, Arun

    2011-01-01

    Various studies have been done to identify antioxidants from plant sources and efforts have been taken to incorporate it in conventional therapy. In our present study, petroleum ether, ethanolic, and aqueous extracts of Rubus ellipticus fruits have been evaluated for in vitro antioxidant activity using DPPH radical scavenging and reducing power assay. BHA was used as a standard antioxidant for DPPH radical scavenging activity. The reducing power assay of extracts was carried out with ascorbic...

  4. Cytotoxic and antioxidant activity of selected marine sponges

    Directory of Open Access Journals (Sweden)

    Chairman K

    2012-06-01

    Full Text Available Objective: To evaluate the anticancer activity of the crude extracts of Rhabdastrella globostellata (R. globostellata and Spirastrella inconstans (S. inconstans var. moeandrina Dendy. Methods: Soxhlet extraction method was used to extract the secondary metabolites and various assays antioxidant, anticancer and various assays were carried out. The extract were tested anticancer activity against a HeLa, Raw 264.7 and Hek-293. Results: The sponge extracts tested exhibited from median to high toxicity in at least one of the toxicity bioassays performed. The antioxidant activity of the isolated metabolite in ethylacetate solution was assessed by SOD and GTH assays and compared with that of other known natural antioxidants. Conclusions: Potent antioxidants have been detected among both phenolic metabolites and alkaloids. Antioxidant effects of tested compounds have been attributed to their action as chain-breaking antioxidants and/or as scavengers of radicals

  5. QSAR models for antioxidant activity of new coumarin derivatives.

    Science.gov (United States)

    Erzincan, P; Saçan, M T; Yüce-Dursun, B; Danış, Ö; Demir, S; Erdem, S S; Ogan, A

    2015-01-01

    This study presents 37 new antioxidant coumarin derivatives and strategies for structural modification to improve their antioxidant activities, the main ferric-reducing antioxidant power (FRAP) assay used to evaluate their antioxidant properties and the generation of validated quantitative structure-activity (antioxidant activity) relationship (QSAR) models. In an attempt to generate QSAR models, structures of all coumarin derivatives in the data set were fully optimized by semi-empirical PM6 method using SPARTAN 10 software. Descriptors were calculated by DRAGON 6.0 software. Multiple linear regression (MLR) models were developed with different training/test set combinations using QSARINS 2.2.1 software. Robustness, reliability and predictive power of the models were tested by internal and external validations. Applicability domain of the best two-descriptor model (nTR = 30; r(2) = 0.924; RMSETR = 0.213; nTEST = 7; r(2)ext = 0.887; RMSEext = 0.255; CCCext = 0.939) was determined. Descriptors appeared in the model revealed that complexity, H-bond donor and lipophilic character are important parameters in describing the antioxidant activity. Apart from the compounds in the data set, we also designed 31 new antioxidant coumarin derivatives and predicted their antioxidant activity using the best two-descriptor model. Most of these compounds are promising antioxidants.

  6. The antioxidant activity of wild medlar ( Mespilus germanica L.) fruit ...

    African Journals Online (AJOL)

    The medlar is an edible fruit. Modern medicine has recognized its healing properties in the treatment of some diseases. There is no scientific data in the literature about the antioxidant activity of methanol or aqueous extract of medlar fruit, leaf or stem bark. Antioxidant activities of these parts were evaluated by employing six ...

  7. Comparative Study of Antioxidant Activities of the Leaves and Stem ...

    African Journals Online (AJOL)

    Leaves and stem extracts of Ipomoea aquatic Forsk were analysed for their antioxidant activities. Folin-Ciocalteu and AlCl3 methods were used to quantify total phenolic and flavonoid contents while 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical assay was used to examine the antioxidant activity of the extracts.

  8. Phenolic Content, and Antioxidant and Antimicrobial Activities of ...

    African Journals Online (AJOL)

    Erah

    Methods: The content of total phenols, flavonoids and anthocyanins of the alcohol, hydroalcohol and aqueous extracts of ... Keywords: Crataegus oxyacantha L.; Natural phenolic compounds; Antioxidant and antimicrobial activity, Southeast Serbia. ..... Antioxidant activities of Sechium edule (Jacq.) Swart extracts, Food ...

  9. In Vitro Antioxidant Activity of Chitosan Aqueous Solution: Effect of ...

    African Journals Online (AJOL)

    Purpose: To investigate the effect of salt form on the antioxidant activities of chitosan aqueous solution. Methods: The antioxidant activities of chitosan acetate (CS-acetate), chitosan hydroxybenzotriazole (CS-HOBt), chitosan thiamine pyrophosphate (CS-TPP) and chitosan ethylenediaminetetraacetic acid (CS-EDTA) ...

  10. Antioxidant and antibacterial activities of Cladophora glomerata (L ...

    African Journals Online (AJOL)

    Cladophora is one of the largest filamentous green-algal genus and has a widespread distribution in Caspian Sea Coast. This study aimed at assaying the antimicrobial and antioxidant activities of Cladophora glomerata in South of Caspian sea. The antioxidant activity of the extract was investigated, including the total ...

  11. Response of total phenolic content and antioxidant activities of bush ...

    African Journals Online (AJOL)

    The positive health benefits associated with tea are made possible by the antioxidant activity of phenolic compounds present in tea. The total phenolic content and antioxidant activity of bush tea (Athrixia phylicoides DC.) and special tea (Monsonia burkeana) were studied. The extractions were done in triplicate using cold ...

  12. High Levels of Phytophenolics and Antioxidant Activities in Oryza ...

    African Journals Online (AJOL)

    Purpose: To investigate phenolic levels, phytophenolic profiles and total antioxidant activities of Oryza sativa, unpolished Thai rice. Methods: Unpolished Thai rice strains of Leum Phua, Klam, Hawm Nil and Black Rose were measured for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ...

  13. Antioxidant, hypoglycemic and anti-diabetic activities of Ziziphus ...

    African Journals Online (AJOL)

    Antioxidant, hypoglycemic and anti-diabetic activities of Ziziphus spina-christi (L) Willd (Rhamnacae) leaf extract. Amal AM Al-Ghamdi, Abdelaaty A Shahat. Abstract. Purpose: To investigate the antioxidant, hypoglycemic and antidiabetic activities as well as the phenolic composition of Ziziphus spina-christi (ZSC) Methods: ...

  14. Comparative Antioxidant Activity of Hibiscus sabdariffa and Ascorbic ...

    African Journals Online (AJOL)

    Nx 6110

    Lipid peroxidation is becoming a popular biological marker of oxidative stress. Hibiscus sabdariffa has been reported to serve as a herbal remedy for various disease conditions, but studies on its antioxidant activity and the extent to which it acts remain scarce. The antioxidant activity of H. sabdariffa aqueous extracts, an.

  15. Antimicrobial And Antioxidant Activities Of Some Nigerian Medicinal ...

    African Journals Online (AJOL)

    Ten Nigerian plants suggested from their ethnomedical uses to possess antimicrobial and antioxidant activities were studied for their anti-microbial and anti-oxidant properties. Antimicrobial activity was tested against Escherichia coli NCTC 10418, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, ...

  16. Extraction and Antioxidant Activity of Phenolic Compounds from ...

    African Journals Online (AJOL)

    1School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, 2Department of Food Science, Xinyang. College of Agriculture ... Purpose: To investigate the extraction and antioxidant activity of phenolic compounds from Okra flowers. ..... antioxidant activities of essential oil and methanol extract of ...

  17. Comparative Antioxidant Activity of Hibiscus sabdariffa and Ascorbic ...

    African Journals Online (AJOL)

    Lipid peroxidation is becoming a popular biological marker of oxidative stress. Hibiscus sabdariffa has been reported to serve as a herbal remedy for various disease conditions, but studies on its antioxidant activity and the extent to which it acts remain scarce. The antioxidant activity of H. sabdariffa aqueous extracts, ...

  18. Antioxidant Activities of Methanol Extract and Solvent Fractions of ...

    African Journals Online (AJOL)

    Purpose: To determine the antioxidant activity of methanol extract (ME) and solvent fractions of Avrainvillea erecta as well as their total phenolic and flavonoid contents. Methods: The antioxidant activities of ME as well as its chloroform, butanol, and aqueous fractions (CF, BF and WF, respectively) of A. erecta were ...

  19. Antioxidant activities of solvent extracts from endemic Cyclamen ...

    African Journals Online (AJOL)

    Leaves extracts of CM exhibited higher antioxidant activity than tuber extracts with all the types of solvent used. All concentrations of petroleum ether, acetone, methanol and water extracts of CM leaves showed higher antioxidant activities than that of 0.5 mg of á-tocopherol (42%) and close to BHT (99.30%) and had 96.60, ...

  20. Application of KRL test to assess total antioxidant activity in pigs: sensitivity to dietary antioxidants.

    Science.gov (United States)

    Rossi, Raffaella; Pastorelli, Grazia; Corino, Carlo

    2013-04-01

    The application of Kit Radicaux Libres (KRL) test to assess total blood antioxidant activity in pigs was evaluated. The KRL has been validated and is widely used in humans for assessing the effectiveness of natural or pharmaceutical treatments, and in vitro to evaluate the antioxidant activities of natural or synthetic antioxidants. In this study the sensitivity of the KRL test in assessing the effectiveness of dietary antioxidant supplementation (vitamin E and plant extract) was evaluated in two different phases of pig breeding. The first trial, in post-weaned piglets (40 piglets/group) fed dietary vitamin E supplementation for 60 days, indicated that there was a higher total antioxidant activity (P=0.032) of whole blood and of red blood cells (P=0.001) than for control pigs. The second trial indicated that long-term supplementation of water soluble plant extract (20 pigs/group) from the leaves of Verbenaceae (Lippia spp.) tended (P=0.091) to increase antioxidant activity in the whole blood of treated, rather than control pigs. These results indicate that the KRL might be recommended as one of efficient means for evaluating antioxidant activity of dietary ingredients fed to pigs. Copyright © 2012 Elsevier Ltd. All rights reserved.

  1. Antioxidative activity of Silibum marianum cultivated in Mongolia

    Directory of Open Access Journals (Sweden)

    J Ganbaatar

    2014-12-01

    Full Text Available Silibum marianum (Milk thistle contains high amount of phenolic compounds with antioxidant properties. The aim of this work was to evaluate the antioxidant activity of  phenolic compounds included in Milk thistle. The  antioxidant properties of the leaves and seeds of milk thistle were examined by determining its ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH free radical. The results obtained from DPPH assay confirm that Milk thistle extracts have high antioxidative activity.DOI: http://doi.dx.org/10.5564/mjc.v15i0.323 Mongolian Journal of Chemistry 15 (41, 2014, p53-55

  2. In vitro protective effects of Thymus quinquecostatus Celak extracts on t-BHP-induced cell damage through antioxidant activity.

    Science.gov (United States)

    Kim, Yon-Suk; Lee, Seung-Jae; Hwang, Jin-Woo; Kim, Eun-Kyung; Kim, Seong-Eun; Kim, Ee-Hwa; Moon, Sang-Ho; Jeon, Byung-Tae; Park, Pyo-Jam

    2012-11-01

    The purpose of this study was to evaluate the antioxidative activities of water and 70% ethanolic extracts from the Thymus quinquecostatus Celak (TQC) for natural antioxidant source. The antioxidant activities were compared with other natural and synthetic antioxidants. The levels of total polyphenols and flavonoids were also determined. The extracts were found to have different levels of antioxidant properties in a few kind of assay. The results showed that higher radical scavenging activity, reducing power and antioxidant capacity in FRAP than those of BHT as a positive control. In addition, the extracts from the TQC leaf and stem showed stronger antioxidant activity than that of vitamin C, α-tocopherol in ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. Cytoprotective and anti-apoptotic effect of water extracts from TQC was also prevented t-BHP-induced toxicity in Chang liver cells. Therefore, these results indicate that TQC extracts have antioxidant properties through its ability to enhance the cell viability, reduction of production of ROS, inhibition of oxidative damage, mitochondria dysfunction and ultimately inhibition of cell apoptosis. Based on the results described above, it is suggested that TQC has the potential to protect liver on t-BHP-induced cell damage and should be considered as a prospective functional food. Copyright © 2012 Elsevier Ltd. All rights reserved.

  3. Antioxidant activities of protein hydrolysates obtained from the housefly larvae.

    Science.gov (United States)

    Zhang, Huan; Wang, Pan; Zhang, Ai-Jun; Li, Xuan; Zhang, Ji-Hong; Qin, Qi-Lian; Wu, Yi-Jun

    2016-09-01

    The housefly is an important resource insect and the housefly larvae are ideal source of food additives. The housefly larvae protein hydrolysates were obtained by enzymatic hydrolysis by alcalase and neutral proteinase. Their antioxidant activities were investigated, including the superoxide and hydroxyl radicalscavenging activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, reducing power and metal chelating activity. The antioxidant activities of both hydrolysates increased with their increasing concentrations. The alcalase hydrolysate (AH) showed higher scavenging activities against hydroxyl radical and superoxide anion radical at low concentrations and higher metal-chelating activity than the neutral proteinase hydrolysate (NPH). The NPH exhibited higher scavenging activity against DPPH free radical and higher reducing power than the AH. Both hydrolysates showed more than 50% superoxide anion radical-scavenging activity at 10 μg/mL. These results indicate that both housefly larvae protein hydrolysates display high antioxidant activities and they could serve as potential natural antioxidant food additives.

  4. Study on the antioxidant activity and membrane interaction of a multiple antioxidant

    DEFF Research Database (Denmark)

    Jodko-Jodko-Piórecka, Kasia; Klösgen, Beate; Litwinienko, Grzegorz

    Catecholamines, including dopamine, have a role in the transduction of nervous stimuli. Apart from that, they might behave as endogenous phenolic antioxidants protecting the neuronal tissue from deleterious effects of oxidative stress. Results from our preliminary study1 even indicate a synergistic...... effect: an interplay of catecholamines with other molecules seems to enhance their antioxidant activity (e.g. 3,4-dihydroxyphenylalanine - L-DOPA -, and 2,2,5,7,8-pentamethyl-6-hydroxychroman - PMHC -, an analogue of α-tocopherol). Addition of small amounts of catecholamines suppresses or largely...... inhibits the peroxidation of lipids2. The understanding of the mechanism of antioxidant action of catecholamines and their interplay with other antioxidants in lipid membranes requires interdisciplinary research on the kinetics and thermodynamics of antioxidant(s)/membrane interactions. Here we report...

  5. Thai Fruits Exhibit Antioxidant Activity and Induction of Antioxidant Enzymes in HEK-293 Cells.

    Science.gov (United States)

    Anantachoke, Natthinee; Lomarat, Pattamapan; Praserttirachai, Wasin; Khammanit, Ruksinee; Mangmool, Supachoke

    2016-01-01

    The cellular antioxidant enzymes play the important role of protecting the cells and organisms from the oxidative damage. Natural antioxidants contained in fruits have attracted considerable interest because of their presumed safety and potential nutritional value. Even though antioxidant activities of many fruits have been reported, the effects of phytochemicals contained in fruits on the induction of antioxidant enzymes in the cells have not been fully defined. In this study, we showed that extracts from Antidesma ghaesembilla , Averrhoa bilimbi , Malpighia glabra , Mangifera indica, Sandoricum koetjape , Syzygium malaccense, and Ziziphus jujuba inhibited H 2 O 2 -induced intracellular reactive oxygen species production in HEK-293 cells. Additionally, these Thai fruit extracts increased the mRNA and protein expressions of antioxidant enzymes, catalase, glutathione peroxidase-1, and manganese superoxide dismutase. The consumption of Thai fruits rich in phenolic compounds may reduce the risk of oxidative stress.

  6. Thai Fruits Exhibit Antioxidant Activity and Induction of Antioxidant Enzymes in HEK-293 Cells

    Directory of Open Access Journals (Sweden)

    Natthinee Anantachoke

    2016-01-01

    Full Text Available The cellular antioxidant enzymes play the important role of protecting the cells and organisms from the oxidative damage. Natural antioxidants contained in fruits have attracted considerable interest because of their presumed safety and potential nutritional value. Even though antioxidant activities of many fruits have been reported, the effects of phytochemicals contained in fruits on the induction of antioxidant enzymes in the cells have not been fully defined. In this study, we showed that extracts from Antidesma ghaesembilla, Averrhoa bilimbi, Malpighia glabra, Mangifera indica, Sandoricum koetjape, Syzygium malaccense, and Ziziphus jujuba inhibited H2O2-induced intracellular reactive oxygen species production in HEK-293 cells. Additionally, these Thai fruit extracts increased the mRNA and protein expressions of antioxidant enzymes, catalase, glutathione peroxidase-1, and manganese superoxide dismutase. The consumption of Thai fruits rich in phenolic compounds may reduce the risk of oxidative stress.

  7. Phytochemical constituents and antioxidant activities of aqueous ...

    African Journals Online (AJOL)

    USER

    2010-08-02

    Aug 2, 2010 ... 0.05) than methanol extract (77.00 ± 0.84%). Nevertheless, methanol and aqueous extracts of C. afer possess anti-oxidative properties as well as bioactive metabolites. Thus, stem extracts of C. afer could serve as sources of antioxidants and bioactive compounds for nutrition and therapeutic purposes.

  8. Physiochemical composition and antioxidant activities of ...

    African Journals Online (AJOL)

    USER

    2010-07-12

    Jul 12, 2010 ... Dietary fiber consumption regardless of soluble as well as insoluble fibers was reported to have positive effects in lowering the risk of cardiovascular disease, gastrointestinal disease, colon cancer and obesity. (Rosamond, 2002). Antioxidant properties of MPP and MPJP. Antioxidant constituents.

  9. Relationship between Estradiol and Antioxidant Enzymes Activity of Ischemic Stroke

    Directory of Open Access Journals (Sweden)

    Nasrin Sheikh

    2009-01-01

    Full Text Available Some evidence suggests the neuroprotection of estrogen provided by the antioxidant activity of this compound. The main objective of this study was to determine the level of estradiol and its correlation with the activity of antioxidant enzymes, total antioxidant status and ferritin from ischemic stroke subjects. The study population consisted of 30 patients with acute ischemic stroke and 30 controls. There was no significant difference between estradiol in stroke and control group. The activity of superoxide dismutase and level of ferritin was higher in stroke compared with control group (<.05, <.001, resp.. There was no significant correlation between estradiol and glutathione peroxidase, glutathione reductase, catalase, total antioxidant status, and ferritin in stroke and control groups. We observed inverse correlation between estradiol with superoxide dismutase in males of stroke patients (=−0.54, =.029. Our results supported that endogenous estradiol of elderly men and women of stroke or control group has no antioxidant activity.

  10. Study of Stevia rebaudiana Bertoni antioxidant activities and cellular properties.

    Science.gov (United States)

    Bender, Cecilia; Graziano, Sara; Zimmermann, Benno F

    2015-01-01

    The aim of our study was to determine the antioxidant activities, cytotoxicity and proliferative properties in Stevia rebaudiana leaves and stems. Leaves extracts exhibited a higher antioxidant activity than stems extract, through oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. Stevioside and rebaudioside A, the main sweetening metabolites in stevia leaves, exhibited a low ORAC value in comparison with plant extracts, while did not elicit any CAA. Stevia rebaudiana did not exhibit toxicity against HepG2 (hepatocellular carcinoma) human cells. No proliferative nor catalase modulations were observed in cells treated with such extracts. Our findings support the promising role of stevia that, apart from its sweetness, can act as a source of antioxidants, even at the intracellular level. This activity makes S. rebaudiana crude extract an interesting resource of natural sweetness with antioxidant properties which may find numerous applications in foods and nutritional supplements industries.

  11. Antioxidant activity of wine assessed by different in vitro methods

    Directory of Open Access Journals (Sweden)

    Di Lorenzo Chiara

    2017-01-01

    Full Text Available Epidemiological studies have suggested that a diet rich in antioxidant compounds could help in counteracting the effects of reactive oxygen species, reducing the risk factors for chronic diseases. The moderate consumption of wine, especially red wine, has been associated with the reduction in mortalities from cardiovascular diseases. One of the possible reasons for the protective effect of wine can be identified in the high content of polyphenols (mainly flavonoids, which have significant antioxidant activity. Even though several in vitro tests have been developed for the measure of the antioxidant property, no method has showed a satisfactory correlation with the in vivo situation. On these bases, the aim of this study was the application and comparison of different in vitro methods to assess the antioxidant activity of red, rosé and white wines. The methods were: 1 Folin-Cocalteau's assay for the quantification of total polyphenol content; 2 the DPPH (1,1-diphenyl-2-picrylhydrazyl spectrophotometric assay and the Trolox Equivalent Antioxidant Capacity (TEAC spectrophotometric assay for measuring the antioxidant activity of samples; 3 High Performance Thin Layer Chromatography for separation of phenolic substances and assessment of the associated antioxidant activity; 4 electrochemical detection by using a biosensor. Although all the approaches show some limitations, this battery of tests offers a more reliable body of data on the antioxidant activity of vine derivatives.

  12. Reviews on Mechanisms of In Vitro Antioxidant Activity of Polysaccharides

    Science.gov (United States)

    Wang, Junqiao; Hu, Shuzhen; Nie, Shaoping; Yu, Qiang; Xie, Mingyong

    2016-01-01

    It is widely acknowledged that the excessive reactive oxygen species (ROS) or reactive nitrogen species (RNS) induced oxidative stress will cause significant damage to cell structure and biomolecular function, directly or indirectly leading to a number of diseases. The overproduction of ROS/RNS will be balanced by nonenzymatic antioxidants and antioxidant enzymes. Polysaccharide or glycoconjugates derived from natural products are of considerable interest from the viewpoint of potent in vivo and in vitro antioxidant activities recently. Particularly, with regard to the in vitro antioxidant systems, polysaccharides are considered as effective free radical scavenger, reducing agent, and ferrous chelator in most of the reports. However, the underlying mechanisms of these antioxidant actions have not been illustrated systematically and sometimes controversial results appeared among various literatures. To address this issue, we summarized the latest discoveries and advancements in the study of antioxidative polysaccharides and gave a detailed description of the possible mechanisms. PMID:26682009

  13. Measurement of Antioxidant Activity Towards Superoxide in Natural Waters.

    Directory of Open Access Journals (Sweden)

    D. Whitney King

    2016-11-01

    Full Text Available Antioxidants are a class of molecules that provide a protective function against reactive oxygen species (ROS in biological systems by out competing physiologically important molecules for ROS oxidation. In natural waters, the reactivity of antioxidants gives an estimate of oxidative stress and may determine the reactivity and distribution of reactive oxidants. We present an analytical method to measure antioxidant activity in natural waters through the competition between ascorbic acid, an antioxidant, and MCLA, a chemiluminescent probe for superoxide. A numerical kinetic model of the analytical method has been developed to optimize analytical performance. Measurements of antioxidant concentrations in pure and seawater are possible with detection limits below 0.1 nM. Surface seawater samples collected at solar noon contained over 0.4 nM of antioxidants and exhibited first-order decay with a half-life of 3-7 minutes, consistent with a reactive species capable of scavenging photochemically produced superoxide.

  14. Antioxidant Activity of Natural Products against Aluminium Fluoride ...

    African Journals Online (AJOL)

    Evaluation of antioxidant potential of natural products against Aluminium fluoride (AlF4) induced oxidative stress in albino mice were represented in the present study. Gossypin, Quercetin dehydrate, (-)-Epicatechin gallate, Gallic acid and Suramin sulphate (G-protein inhibitor) were evaluated for antioxidant activity by ...

  15. Cytotoxic and Antioxidant Activities of Antidesma thwaitesianum Müll ...

    African Journals Online (AJOL)

    natural antioxidants; however, no specific report regarding health benefits of Antidesma thwaitesianum fruits has been published to date. Thus, the present study is the first to determine the cytotoxic and antioxidant activities of mamao fruit and fruit waste extracts obtained by different extraction methods using chemical and ...

  16. Determination of secondary metabolites and antioxidant activity of ...

    African Journals Online (AJOL)

    and test samples, respectively. IC50, which is the concentration required to obtain a 50 % antioxidant capacity, was determined from a graph of antioxidant activity (%) against extract concentration (µg /mL). Total phenolic contents. The total phenolic content of the samples was determined by the Folin-Ciocalteu method [13].

  17. Antibacterial and antioxidant activities of the essential oils and ...

    African Journals Online (AJOL)

    The present study deals with the evaluation of the in vitro antioxidant and antibacterial activity of phenolic extracts and essential oils of two medicinal and aromatic plants Zygophyllum album and Myrtus communis by using the 2,2- diphenyl-2-picrylhydrazyl radical, total antioxidant power and agar diffusion methods and ...

  18. Propolis in Kenya: Antioxidative and Radical Scavenging Activity in ...

    African Journals Online (AJOL)

    Dept. of Food Science & Technology

    2013-01-01

    Jan 1, 2013 ... Phenolic compounds in plants have properties; that influence health and performance enhancement in humans [1]. ... These bioflavonoids are the main antioxidants found in plant extracts. [4]. Natural antioxidants ..... A New Chalcone with a Potent Radical Scavenging Activity and. Accompanying Phenolics ...

  19. Antidepressant and antioxidant activities of Artemisia absinthium L ...

    African Journals Online (AJOL)

    Artemisia absinthium (Asteraceae) is widely used in Iranian traditional medicine. Its effects may be correlated with the presence of antioxidant compounds. Methanolic extract of A. absinthium aerial part at flowering stage was screened for antioxidant activities by five complementary test systems. Also, its antidepressant ...

  20. Investigation of the antioxidant activity and quantification of the ...

    African Journals Online (AJOL)

    It is also rich in carotene, niacin, riboflavin, thiamine and vitamin C. Objectives: To determine the amounts of toxic heavy metals, quantify the amounts of vitamins A and E and investigate the antioxidant activities of Carrot. Method: The research investigated the antioxidant properties of carrot on the basis of the radical ...

  1. Antioxidant Activities of the Leaf Extract and Fractions of Cola ...

    African Journals Online (AJOL)

    Cola lepidota (CL) K. Schum (sterculiaceae) used in Nigeria folk medicine as febrifuges, pulmonary problems and cancer related ailments. This study evaluated scientifically the in vitro antioxidant activity of extract and fractions with a view to validate its folkloric usage. In vitro antioxidant properties of extract and fractions ...

  2. Antioxidant activity of the medicinal plant Coleus forskohlii Briq.

    African Journals Online (AJOL)

    user

    2011-03-28

    Mar 28, 2011 ... Antioxidant status of different parts of Coleus forskohlii including roots, stem, leaves and tubers was analyzed. For the enzymatic antioxidant properties, the activities of superoxide dismutase, peroxidase, polyphenol oxidase and catalase were significantly higher (P < 0.05) in tubers than in the leaves, roots.

  3. Antimicrobial and antioxidant activities of two endemic plants from ...

    African Journals Online (AJOL)

    In addition to the antioxidant activity of these plants, the total phenolic compounds and flavonoids were also measured in the extracts. ... that the extracts of A. scabriflorum and A. tchihatschewii possess antimicrobial and antioxidant properties, and therefore, they can be used as a natural preservative ingredient in food

  4. Antioxidant and free radical scavenging activities of plant extracts ...

    African Journals Online (AJOL)

    Twenty-two species of medicinal plants collected in the Mexican state of Morelos were selected to evaluate their free radical scavenging and antioxidant activities. The extracts from the aerial parts of the plants were obtained using hexane, acetone and methanol (66 extracts). The initial qualitative screening of antioxidants ...

  5. Total Antioxidant Activity, and Hesperidin, Diosmin, Eriocitrin and ...

    African Journals Online (AJOL)

    Total Antioxidant Activity, and Hesperidin, Diosmin, Eriocitrin and Quercetin Contents of Various Lemon Juices. ... Conclusion: The developed method can also be applied to the determination of related extracts of orange cultivars as well as various extracts of medicinal plants. Keywords: Antioxidant, Flavonoid, Hesperidin, ...

  6. Antioxidant Activities and Food Value of Five Underutilized Green ...

    African Journals Online (AJOL)

    ... iron, calcium and vitamin C. The antioxidant activities and phenolic antioxidant contents of the vegetables were also high. The health claims associated with some of these food values and bioactive compounds are noteworthy, thereby underlining the potential role of these underutilized vegetables as functional foods.

  7. Antioxidant and sgc-7901 cell inhibition activities of Rhizoma ...

    African Journals Online (AJOL)

    The objective of this research was to study the pharmacology of Dioscorea bulbifera L. on antioxidant and anticancer activity. Alcohol extracts of Dioscorea bulbifera L. were made out by different concentration alcohol; they were tested by Hydroxyl radical scavenging test, reducing capacity test and total antioxidant capacity ...

  8. Effect of maturity and harvest season on antioxidant activity ...

    African Journals Online (AJOL)

    Antioxidant activity diphenylpicrylhydrazyl (DPPH), the ferric-reducing antioxidant power assay (FRAP), nitric oxide (NO)], total polyphenols, phenolic compounds and ascorbic acid of Morinda citrifolia L. fruits were investigated as a function of maturity and three seasons patterns in Mexico. Maturity was evaluated in early, ...

  9. Antioxidant and antimicrobial activities of Heracleum nepalense D ...

    African Journals Online (AJOL)

    Antioxidant and antimicrobial activities of Heracleum nepalense D Don root. S Dash, L K Nath, S Bhise. Abstract. Purpose: The aim of the present study was to investigate antioxidant and antimicrobial effects of the methanol extract of Heracleum nepalense D.Don roots. Method: The antimicrobial effect was determined by ...

  10. Hepatoprotective and antioxidant activities of fruit pulp of limonia ...

    African Journals Online (AJOL)

    Hepatoprotective and antioxidant activities of fruit pulp of limonia acidissima linn. ... The fruit extract significantly restored the antioxidant enzyme level in the liver cells. Conclusion: MELA exhibited significant dose ... This study rationalized the ethno-medicinal use of the plant for curing hepatic injuries. Keywords: Limonia ...

  11. Modulation of Antioxidant Enzyme Expression and Activity by ...

    African Journals Online (AJOL)

    Renal toxicity produced by paraquat involves the generation of reactive oxygen species (ROS) which can overwhelm antioxidant defences, leading to oxidant injury. However, there are conflicting reports regarding the activity and/or expression of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) ...

  12. Chemical composition and in vitro antioxidant activities of ...

    African Journals Online (AJOL)

    ... and the results were compared to the reference BHT (butyl hydroxy toluene). In the three earlier mentioned assays, the essential oil demonstrated a potential antioxidant which may be considered as potent agent in food preservation and drug discovery. Key words: Thymelaea hirsuta, essential oil, antioxidant activities.

  13. The antioxidant activity of selected Romanian honeys

    Directory of Open Access Journals (Sweden)

    Irina DOBRE

    2010-12-01

    Full Text Available Four types of Apis mellifera honey collected in the eastern region of Romania were screened for their total phenolic content by the Folin-Ciocalteau method, for anti radical power as assessed by DPPHradical scavenging assay, for the FRAP assay-ferric reducing antioxidant power. The antioxidant effect over a substrate sensitive to lipid per oxidation and also the presence of some pigments with antioxidant action in honey-ABS 450 were evaluated. All assays revealed the following order of the obtained values: Lime honey>poly-flower honey>Sea buckthorn honey>Acacia honey.

  14. The study of antioxidant activity of white tea

    Directory of Open Access Journals (Sweden)

    O. V. Chernousova

    2018-01-01

    Full Text Available Antioxidants protect the organism from the harmful effects of free radicals. High content of antioxidants is characteristic for red wine, olive oil, tea. Today in Russia is well studied the antioxidant activity of black and green tea, but the choice of teas found in the sale, much wider. The aim of the work was to study antioxidant activity of white tea, as well as its comparison with the parameters of green tea and rooibos. Spectrophotometrically compared the antioxidant activity of aqueous extracts white, green tea, drink rooibos using the adrenaline autooxidation reaction in alkaline medium (pH 10.65. The correctness of the spectrophotometric determination was confirmed on the device “Tsvet Yauza-01-AA” (Russia. As a standard used quercetin. In the initial period of time (up to 5 min tea extracts “Java”, «Elixir immortality» and rooibos are equally affect the values of optical density. After 10 min the optical density takes different values for different extracts, however this time is recommended to determine antioxidant activity. The highest antioxidant activity of the extract has white tea «Greenfield». Rooibos extract antioxidant activity could be compared with “Elixir of immortality” white tea аctivity. The lowest value of antioxidant activity of green tea “Princess JAVA”. The effect of water temperature on the antioxidant activity of extracts was investigated. The highest value was found in tea «Greenfield» at a brewing temperature of 70 °C, which decreases with increasing temperature to 100 °C. The water temperature has a minimal effect on the antioxidant activity of the rooibos extract, but a small maximum at 80 °C is observed. The minimum value of antioxidant activity is the extract of green tea «Princess JAVA», but it increases with the temperature rise up to 100 °C. The study was conducted according to the content of antioxidants from the brewing time. For this, the device “Tsvet Yauza-01-AA” was

  15. The comparison of antioxidative and hepatoprotective activities of Codonopsis pilosula polysaccharide (CP) and sulfated CP.

    Science.gov (United States)

    Liu, Cui; Chen, Jin; Li, Entao; Fan, Qiang; Wang, Deyun; Li, Peng; Li, Xiuping; Chen, Xingying; Qiu, Shulei; Gao, Zhenzhen; Li, Hongquan; Hu, Yuanliang

    2015-02-01

    Codonopsis pilosula polysaccharide (CP) was extracted, purified and modified by chlorosulfonic acid-pyridine method to obtain a sulfated CP (sCP). Their antioxidative activities in vitro were compared through the free radical-scavenging test. The results demonstrated that the scavenging capabilities of sCP were significantly stronger than those of CP. In vivo test, the mice hepatic injury model was prepared by BCG/LPS method, then administrated respectively with sCP and CP at three dosages, the biochemical indexes in serum, antioxidative indexes in liver homogenate and histopathological change in liver of the mice were compared. The results showed that in high (200mg/kg) and middle (150mg/kg) dosages of sCP groups, the contents of ALT, AST and TNF-α in serum and MDA in liver homogenate were significantly lower than those in the model group and numerically lower than those in the CP groups, the activities of SOD and GSH-Px in liver homogenate were significantly higher than those in the model group and numerically higher than those in the CP groups. In the model group there were obvious pathological changes in the liver, while in the sCP groups were near normal. These results indicate that sCP and CP possess antioxidative activity in vitro and in vivo, the activity of sCP is stronger than that of CP and sulfation modification can enhance the antioxidative and hepatoprotective activities of Codonopsis pilosula polysaccharide. Copyright © 2014 Elsevier B.V. All rights reserved.

  16. Effect of freeze-drying on the antioxidant compounds and antioxidant activity of selected tropical fruits.

    Science.gov (United States)

    Shofian, Norshahida Mohamad; Hamid, Azizah Abdul; Osman, Azizah; Saari, Nazamid; Anwar, Farooq; Dek, Mohd Sabri Pak; Hairuddin, Muhammad Redzuan

    2011-01-01

    The effects of freeze-drying on antioxidant compounds and antioxidant activity of five tropical fruits, namely starfruit (Averrhoa carambola L.), mango (Mangifera indica L.), papaya (Carica papaya L.), muskmelon (Cucumis melo L.), and watermelon Citruluss lanatus (Thunb.) were investigated. Significant (p 0.05) change, however, observed in the ascorbic acid content of the fresh and freeze-dried fruits. Similarly, freeze-drying did not exert any considerable effect on β-carotene concentration of fruits, except for mango and watermelon, where significantly (p correlation was established between the result of TPC and antioxidant assays, indicating that phenolics might have been the dominant compounds contributing towards the antioxidant activity of the fruits tested.

  17. Screening of antioxidant activity in microalgae

    Directory of Open Access Journals (Sweden)

    Mariana F.G. Assunção

    2014-06-01

    Both sets of results indicate an interesting antioxidant potential in microalgae belonging to the groups Eustigmatophyceae and Chlorophyceae. Tested species of these groups showed ABTS•+ values comparable to grape and raspberry ethanolic extracts, confirmed also by the DPPH• method.

  18. Antioxidant activity of fermented broccoli and spinach by Kombucha culture

    Science.gov (United States)

    Artanti, Nina; Susilowati, Agustine; Aspiyanto, Lotulung, Puspa Dewi Narrij; Maryati, Yati

    2017-11-01

    Broccoli (Brassica oleracea L.) and spinach (Amaranthus spp.) are vegetables that known to have many benefit for health. Previous studies on the fermentation of those vegetables using kombucha cultured showed increase in bioactive components such as total polyphenol content. The current studies was performed to evaluate the antioxidant activity of fermented spinach and broccoli before (feed) and after treatment with filtration (retentate and permeate). Filtration was conducted using Stirred Ultrafiltration Cell (SUFC) with UF membrane 100,000 MWCO mode at fixed condition (stirred rotation 300 rpm, room temperature, pressure 40 psia). Antioxidant evaluation was conducted using 2,2-diphenyl-1-picril hydrazyl (DPPH) free radical scavenging activity assay. The results showed that all samples from fermented broccoli showed antioxidant activity (feed 15.82% inhibition and retentate 15.29% inhibition), with the best antioxidant activity was obtained from permeate (75.98% inhibition). Whereas from fermented spinach only permeate showed antioxidant activity (21.84% inhibition) and it significantly lower than broccoli permeate. The mass spectrum of LCMS analysis on broccoli samples showed the present of several mass spectrum with (M+H) range from 148.1 to 442.5 in feed, retentate and permeate. In those samples (M+H) 360.4 always has the highest relative intensity. These results suggest that fermented broccoli has potential for development as functional drink for the source of antioxidant and the permeate obtained from filtration treatment significantly increased the antioxidant activity.

  19. Comparison of antioxidant activity in commercial Ginkgo biloba preparations.

    Science.gov (United States)

    Mantle, David; Wilkins, Richard M; Gok, Muhamed Asim

    2003-10-01

    To compare the relative levels of antioxidant activity in vitro in Ginkgo biloba samples (in tablet or capsule form) from different commercial suppliers, to determine whether some brands may be more efficacious in their potential to increase endogenous antioxidant activity, and thereby counter oxidative stress related disorders. Antioxidant activity of the above sample extracts was determined in vitro against the ABTS.(+) (2-2'-amino-bis-3-ethylbenzthiazoline-6-sulfonic acid) radical, relative to Trolox (water-soluble vitamin E analogue) antioxidant standards, using an established assay procedure. The relative antioxidant activity of G. biloba sample extracts was expressed in terms of millimoles per liter of Trolox equivalent (TE) for the initial extract, micromol TE per whole tablet, nmol TE per mg tablet, and nmol TE per mg ginkgo content. Data (as mean +/- standard deviation (SD) from 4 separate estimations) obtained in this study showed a considerable variation (approximately 50-fold) in the level of antioxidant activity in preparations from different suppliers, particularly when compared on an equivalent (i.e., nmol TE/mg ginkgo) basis. Of the 18 products investigated, the highest level of antioxidant activity (whether expressed as micromol TE per whole tablet or nmol TE/mg ginkgo) was obtained for Pharma Nord Bio-Biloba (Pharma Nord, Morpeth, UK) tablets (p antioxidant activity of the various products investigated can be accounted for in terms of the physical nature of the G. biloba (i.e., dried leaf powder or standardized concentrated extract) used in tablet formulation. However, even when comparing products based on concentrated extract, the data demonstrated that there is still a considerable variation in antioxidant activity. Presumably this may result from differences in the manufacturing process between suppliers, which in turn may limit the efficacy of these preparations in the prevention or treatment of disease.

  20. Antioxidant and Anti-Inflammatory Activities of Barettin

    OpenAIRE

    Lind, Karianne F.; Hansen, Espen; ?sterud, Bjarne; Eilertsen, Karl-Erik; Bayer, Annette; Engqvist, Magnus; Leszczak, Kinga; J?rgensen, Trond ?.; Andersen, Jeanette H.

    2013-01-01

    In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activity. Barettin was also able to inhibit the secretion of the inflammatory cytokines IL-1β and TNFα fr...

  1. Application of radiobiological techniques in studying antioxidant mechanisms: evaluation of their radioprotective, antioxidative and antiviral activities

    International Nuclear Information System (INIS)

    Hmamouchi, M.

    2000-01-01

    In the medical field, the oxidation phenomenon is the source of several pathologies (diabetes, cystic fibrosis, cancers,...). The natural oxidants are used as food preserving and skin ageing moderators. Several plant extracts with antioxidant activity were studied, this important antioxidant activity is probably due to their richness of compounds: polyphenols, phenolic acids, tocopherols, carotenoids, flavonoids,... Many techniques for evaluation and reactional mechanism study of the antioxidative activity are used. After selection, extraction, fractionation, activity screening, chemical analyses of molecules contained in the best active extracts, biological properties research of isolated redox pharmacophore, we have : - determined the structure of active products by spectroscopy and chromatography; - studied the antioxidative properties by EPR and spin trapping of the obtained extracts and molecules. The results of this first part of our work consists in evaluating the antioxidative degree of a great number of natural active principles, extracted from moroccan plants and pur obtained products. The second part consists in studying the action mechanisms using the LDL labelling (F. M.)

  2. Effect of gamma irradiation on antioxidant activity of Amoora rohitaka

    International Nuclear Information System (INIS)

    Rajurkar, N.S.; Gaikwad, K.N.

    2012-01-01

    The effect of a medium dose of gamma radiation on antioxidant activity of Amoora rohitaka was studied. Radiation doses were 0, 1, 3 and 5 kGy. Antioxidant activity was screened by using different assay. With increasing dose the formation of Maillard reaction products (MRPs) contributes to the increase in the antioxidant activity. MRPs are formed as a result of Maillard reaction. In ABTS [2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid di-ammonium salt] assay, ethanol extract showed increase in scavenging activity. It also showed a marked increase in polyphenolic contents. The present study reveals that gamma irradiation can be an efficient process to increase antioxidant activity of Amoora rohitaka. (author)

  3. Synthesis and Antioxidant Activity of Alkyl Nitroderivatives of Hydroxytyrosol

    Directory of Open Access Journals (Sweden)

    Elena Gallardo

    2016-05-01

    Full Text Available A series of alkyl nitrohydroxytyrosyl ether derivatives has been synthesized from free hydroxytyrosol (HT, the natural olive oil phenol, in order to increase the assortment of compounds with potential neuroprotective activity in Parkinson’s disease. In this work, the antioxidant activity of these novel compounds has been evaluated using Ferric Reducing Antioxidant Power (FRAP, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS, and Oxygen Radical Scavenging Capacity (ORAC assays compared to that of nitrohydroxytyrosol (NO2HT and free HT. New compounds showed variable antioxidant activity depending on the alkyl side chain length; compounds with short chains (2–4 carbon atoms maintained or even improved the antioxidant activity compared to NO2HT and/or HT, whereas those with longer side chains (6–8 carbon atoms showed lower activity than NO2HT but higher than HT.

  4. Chemical composition and antioxidant activity of Origanum vulgare subsp. vulgare essential oil from Iran

    Directory of Open Access Journals (Sweden)

    M. Vazirian

    2015-12-01

    Full Text Available Background and Objectives: Essential oils are very complex mixture of components and their composition may vary in different species or varieties or even within the same variety. Origanum vulgare L. subsp. vulgare is one of the most distributed subspecies within the genus Origanum and has been found to be a poor-oil, categorized in cymyl, bornane or sabinyl chemotypes with higher proportion of sesquiterpenes. In this experiment, the Iranian sample was studied for the chemical composition of the oil and evaluation of its antioxidant activity. Methods: Essential oil was obtained by hydro-distillation and analyzed by GC/MS for determination of components. Antioxidant activity was evaluated by radical scavenging ability (DPPH method and reducing power (FRAP assay. Results: The sample belonged to “thymol” chemotype with the main components as thymol (37.13%, gama-terpinene (9.67%, carvacrol (9.57%, carvacrol methyl ether (6.88, cis-alpha-bisabolene (6.80%, eucalyptol (3.82%, p-cymene (3.58% and elemol (2.04%. The oil of plant showed very strong antioxidant activity (IC50=2.5 µg/mL in DPPH method, which was stronger than the standard antioxidants (Vit E and BHA, p

  5. Enhanced antioxidant activity of polyolefin films integrated with grape tannins.

    Science.gov (United States)

    Olejar, Kenneth J; Ray, Sudip; Kilmartin, Paul A

    2016-06-01

    A natural antioxidant derived from an agro-waste of the wine industry, grape tannin, was incorporated by melt blending into three different polyolefins (high-density polyethylene, linear low-density polyethylene and polypropylene) to introduce antioxidant functionality. Significant antioxidant activity was observed at 1% tannin inclusion in all polymer blends. The antioxidant activity was observed to increase steadily with a greater concentration of grape tannins, the highest increases being seen with polypropylene. The mechanical and thermal properties of the polymer films following antioxidant incorporation were minimally altered with up to 3% grape tannins. All of the polyolefin-grape tannin films successfully passed the leachability test following USP661 standard protocol. Superior antioxidant activity was established in polyolefin thin films by utilization of a bulk grape extract obtained from winery waste. Significant increases in antioxidant activity were seen with 1% extract inclusion. This not only demonstrates the potential for food packaging applications of the polyolefin blends, but also valorizes the agro-waste. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  6. Antioxidant activity in selected Slovenian organic and conventional crops

    Directory of Open Access Journals (Sweden)

    Manca KNAP

    2015-12-01

    Full Text Available The demand for organically produced food is increasing. There is widespread belief that organic food is substantially healthier and safer than conventional food. According to literature organic food is free of phytopharmaceutical residues, contain less nitrates and more antioxidants. The aim of the present study was to verify if there are any differences in the antioxidant activity between selected Slovenian organic and conventional crops. Method of DPPH (2,2-diphenyl-1-picryhydrazyl was used to determine the antioxidant activity of 16 samples from organic and conventional farms. The same varieties of crops were analysed. DPPH method was employed to measure the antioxidant activity of polar antioxidants (AAp and antioxidant activity of fraction in ethyl acetate soluble antioxidants (EA AA. Descriptive statistics and variance analysis were used to describe differences between farming systems. Estimated differences between interactions for the same crop and different farming practice were mostly not statistically significant except for the AAp for basil and beetroot. Higher statistically significant values were estimated for conventional crops. For the EA AA in broccoli, cucumber, rocket and cherry statistically significant higher values were estimated for organic production.

  7. A Caco-2 cell-based quantitative antioxidant activity assay for antioxidants.

    Science.gov (United States)

    Wan, Hongxia; Liu, Dong; Yu, Xiangying; Sun, Haiyan; Li, Yan

    2015-05-15

    A Caco-2 cell-based antioxidant activity (CAA) assay for quantitative evaluation of antioxidants was developed by optimizing seeding density and culture time of Caco-2 cells, incubation time and concentration of fluorescent probe (2',7'-dichlorofluorescin diacetate, DCFH-DA), incubation way and incubation time of antioxidants (pure phytochemicals) and DCFH-DA with cells, and detection time of fluorescence. Results showed that the CAA assay was of good reproducibility and could be used to evaluate the antioxidant activity of antioxidants at the following conditions: seeding density of 5 × 10(4)/well, cell culture time of 24h, co-incubation of 60 μM DCFH-DA and pure phytochemicals with Caco-2 cells for 20 min and fluorescence recorded for 90 min. Additionally, a significant correlation was observed between CAA values and rat plasma ORAC values following the intake of antioxidants for selected pure phytochemicals (R(2) = 0.815, p < 0.01), demonstrating the good biological relevance of CAA assay. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich ...

    African Journals Online (AJOL)

    Antioxidative and Hepatoprotective Activities of Deinoxanthin-Rich Extract from Deinococcus radiodurans R1 against Carbon Tetrachloride-Induced Liver Injury in Mice. J Cheng, Z Zhang, Z Zheng, G Lv, L Wang, B Tian, Y Hua ...

  9. Antioxidant and antibacterial activities of ethanolic extracts of ...

    African Journals Online (AJOL)

    Antioxidant and antibacterial activities of ethanolic extracts of Asparagus officinalis cv. Mary Washington: Comparison of in vivo and in vitro grown plant bioactivities. Arash Khorasani, Wirakarnain Sani, Koshy Philip, Rosna Mat Taha, Arash Rafat ...

  10. Antioxidant activity of black bean (Phaseolus vulgaris L. protein hydrolysates

    Directory of Open Access Journals (Sweden)

    Jarine Amaral do EVANGELHO

    2016-01-01

    Full Text Available Abstract The objective of this work was to study the effect of enzymatic hydrolysis of black bean protein concentrate using different enzymes. Bean proteins were extracted and hydrolyzed over a period of 120 min using the enzymes pepsin or alcalase. The protein hydrolysates’ molecular weight was assayed by electrophoresis and the antioxidant activity was evaluated by the capturing methods of free radicals ABTS●+ and DPPH. Electrophoretic results showed that the bands above 50 kDa disappeared, when the beans protein was subjected to hydrolysis with pepsin. The bean protein hydrolysate obtained by hydrolysis with alcalase enzyme, showed higher antioxidant activity for inhibition of the radical ABTS●+. However, the hydrolysates obtained by hydrolysis with pepsin had higher antioxidant activity for inhibition of the radical DPPH. The use of pepsin and alcalase enzymes, under the same reaction time, produced black bean protein hydrolysates with different molecular weight profiles and superior antioxidant activity than the native bean protein.

  11. Antioxidant activities of the selected plants from the family ...

    African Journals Online (AJOL)

    . Antioxidant activities of these extracts were evaluated through DPPH• radical scavenging, phosphomolybdate and ferric thiocyanate (FTC) methods. Methanolic extract of Cinnamomum zeylanicum and Cinnamomum tamala showed highest ...

  12. In vitro Cytotoxic and Antioxidant Activity of Leaf Extracts of ...

    African Journals Online (AJOL)

    In vitro Cytotoxic and Antioxidant Activity of Leaf Extracts of Mangrove Plant, Phoenix paludosa Roxb. Sameera R. Samarakoon, Chanthirika Shanmuganathan, Meran K. Ediriweera, Kamani H. Tennekoon, Poorna Piyathilaka, Ira Thabrew, E Dilip de Silva ...

  13. Purification, composition analysis and antioxidant activity of different ...

    African Journals Online (AJOL)

    Purification, composition analysis and antioxidant activity of different polysaccharides from the fruiting bodies of Pholiota adiposa. Yongxin Nie, Hongxia Jiang, Yanyou Su, Changxiang Zhu, Jiarui Li, Fujiang Wen ...

  14. Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts

    Czech Academy of Sciences Publication Activity Database

    Denev, P.; Kratchanova, M.; Číž, Milan; Lojek, Antonín; Vašíček, Ondřej; Blazheva, D.; Nedelcheva, P.; Vojtek, L.; Hyršl, P.

    2014-01-01

    Roč. 61, č. 2 (2014), s. 359-367 ISSN 0001-527X Institutional support: RVO:68081707 Keywords : herbs * polyphenols * antioxidant activity Subject RIV: BO - Biophysics Impact factor: 1.153, year: 2014

  15. Studies of the structure-antioxidant activity relationships and antioxidant activity mechanism of iridoid valepotriates and their degradation products.

    Science.gov (United States)

    Wang, Feifei; Zhang, Yumei; Wu, Shouhai; He, Yi; Dai, Zhong; Ma, Shuangcheng; Liu, Bin

    2017-01-01

    Oxidative stress has been associated with diverse diseases, including obesity, cancer and neurodegeneration. In fact, Valeriana jatamansi Jones (valerian) and its extracts possess strong antioxidant activities that extend their application in clinical practice to the treatment of these illnesses, even though the underlying mechanisms are not well understood. Iridoid valepotriate, a characteristic iridoid ester in valerian with poor chemical stability, possesses considerable antioxidant components. The original compounds and their degradation products have been found to exhibit strong antioxidant activities. However, the relationship between their structure and antioxidant effects and the mechanism underlying their oxidation resistance remain unclear. A forced degradation study using three iridoid valepotriates (valtrate, acevaltrate and 1-β acevaltrate) was performed in this work, and the structures of their degradation products were estimated by TLC-MS and LC-MS. Comparison of the antioxidant activities of the iridoid valepotriates before and after forced degradation revealed that degradation reduced the activities of the iridoid valepotriates in free radical scavenging and cytotoxic and cell apoptosis tests. The results suggested that the oxirane nucleus is important for defining the antioxidant profile of iridoid valepotriate. We uncovered possible mechanisms that could explain the antioxidant activities, including the generation of two hydroxyl groups through intramolecular transfer of an H• from an oxirane ring and a reduction in ROS levels through interactions with GABAergic signalling pathways.

  16. Studies of the structure-antioxidant activity relationships and antioxidant activity mechanism of iridoid valepotriates and their degradation products

    Science.gov (United States)

    Wang, Feifei; Zhang, Yumei; Wu, Shouhai; He, Yi; Dai, Zhong; Liu, Bin

    2017-01-01

    Oxidative stress has been associated with diverse diseases, including obesity, cancer and neurodegeneration. In fact, Valeriana jatamansi Jones (valerian) and its extracts possess strong antioxidant activities that extend their application in clinical practice to the treatment of these illnesses, even though the underlying mechanisms are not well understood. Iridoid valepotriate, a characteristic iridoid ester in valerian with poor chemical stability, possesses considerable antioxidant components. The original compounds and their degradation products have been found to exhibit strong antioxidant activities. However, the relationship between their structure and antioxidant effects and the mechanism underlying their oxidation resistance remain unclear. A forced degradation study using three iridoid valepotriates (valtrate, acevaltrate and 1-β acevaltrate) was performed in this work, and the structures of their degradation products were estimated by TLC-MS and LC-MS. Comparison of the antioxidant activities of the iridoid valepotriates before and after forced degradation revealed that degradation reduced the activities of the iridoid valepotriates in free radical scavenging and cytotoxic and cell apoptosis tests. The results suggested that the oxirane nucleus is important for defining the antioxidant profile of iridoid valepotriate. We uncovered possible mechanisms that could explain the antioxidant activities, including the generation of two hydroxyl groups through intramolecular transfer of an H• from an oxirane ring and a reduction in ROS levels through interactions with GABAergic signalling pathways. PMID:29232391

  17. Antioxidant Activity of Water-soluble Polysaccharides from Brasenia schreberi

    OpenAIRE

    Xiao, Huiwen; Cai, Xueru; Fan, Yijun; Luo, Aoxue

    2016-01-01

    Objective: In order to investigate the antioxidant activities of polysaccharides (BPL-1 and BPL-2), one of the most important functional constituents in Brasenia schreberi was isolated from the external mucilage of B. schreberi (BPL-1) and the plant in vivo (BPL-2). This paper examines the relationship between the content of sulfuric radicals and uronic acid in BPL and the antioxidant activity of BPL. Materials and Methods: The free radicals, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic ...

  18. [Effect of antioxidants with different antiradical activity on cornea preservation].

    Science.gov (United States)

    Travkin, A G; Vinetskaia, M I; Slepukhina, L V

    1976-11-01

    Changes of chemoluminescence intensity, collagen mucopolysaccaride content in the rabbit eye cornea were studied on 32 cornea of shinshilla eye conservated by dehydration under hipotenia (2 divided by 4 degrees C) for 60 days with the application of antioxidants (tocoferol, chlorhydrate 2-ethyl, 6-methyl-3-oxipyridine) having different antiradical activity. It is concluded that the application of natural antioxidant alpha-tocoferol having high antiradical activity is expedient for the conservation of cornea.

  19. Antioxidative and Anticholinesterase Activity of Cyphomandra betacea Fruit

    OpenAIRE

    Ali Hassan, Siti Hawa; Abu Bakar, Mohd Fadzelly

    2013-01-01

    Cyphomandra betacea is one of the underutilized fruits which can be found in tropical and subtropical countries. This study was conducted to determine the antioxidant activity and phytochemical contents in different parts (i.e., flesh and peel) of the fruits. Antioxidants were analyzed using DPPH and ABTS free radical scavenging assays as well as FRAP assay. Anticholinesterase activity was determined using enzymatic assay using acetyl cholinesterase enzyme. For 80% methanol extract, the peel ...

  20. Phytochemical study, antioxidant and antibacterial activities of Stemodia maritima

    Directory of Open Access Journals (Sweden)

    Francisca R. L. da Silva

    2014-01-01

    Full Text Available Stemodinol, a new natural compound, together with known compounds including jaceidin, stemodin, stemodinoside B, isocrenatoside, verbascoside, crenatoside, and isoverbascoside, were isolated from Stemodia maritima Linn. The antioxidant (DPPH method and antimicrobial activities of stemodin, stemodinoside B, and crenatoside were investigated. Among the components tested, only crenatoside isolated from the roots showed a high antioxidant power. Stemodin and stemodinoside B exhibited antibacterial activities.

  1. Edible flowers - antioxidant activity and impact on cell viability

    OpenAIRE

    Kuceková, Zdenka; Mlček, Jiří; Humpolíček, Petr; Rop, Otakar

    2013-01-01

    The phenolic compound composition, antioxidant activity and impact on cell viability of edible flower extracts of Allium schoenoprasum; Bellis perennis; Cichorium intybus; Rumex acetosa; Salvia pratensis; Sambucus nigra; Taraxacum officinale; Tragopogon pratensis; Trifolium repens and Viola arvensis was examined for the first time. Total phenolic content of the flowers of these plants fell between 11.72 and 42.74 mg of tannin equivalents/kg of dry matter. Antioxidant activity ranged from 35.5...

  2. Antioxidant activity of commonly consumed vegetables in yemen.

    Science.gov (United States)

    Al-Mamary, Mohammed Ali

    2002-09-01

    Of late, more attention has been paid to the role of natural antioxidants, especially phenolic compounds, which may act both by reducing the content of toxic compounds in foods and by supplying the human body with exogenous antioxidants. The total phenolic contents and the total antioxidant activities (% AA) of 17 commonly consumed vegetables in Yemen were determined. Total phenolics was measured colorimetrically using the Folin-Ciocalteu reagent with D-catechin as the standard. The antioxidant activity (%AA) of vegetables using their juices at different levels (50μl, 100μl, 200μl) was assayed in vitro by the inhibition of liver homogenate oxidation mediated by FeSO4 / ascorbate system. The % AA was measured by the TBAR method. The results showed that for all vegetable juices, increased antioxidant activity or decreased prooxidant activity increased with volume of juice. With the addition of 50μl from each sample, most vegetable juices showed antioxidant activity and it appears that coriander had exceptionally high antioxidant activity (60%). However, garlic, yellow onion, parsley, red onion and Cissus rotundifolia showed considerably high antioxidant activities (54-41 %). Other vegetables with low antioxidant activities (27 to 3 %) showed an order of increase in %AA as follows: tomato > spinach > horseradish-leaves > carrot > cabbage > chilli > leek > horseradish-roots. At the same, time some vegetable juices such as that of green pepper, cucumber and lettuce showed initial prooxidant activities (-11 to -40 %). The complete inhibition of oxidation was noticed when the highest level of juices (200μl) was used in the cases of coriander, onions, garlic and parsley. The correlation between total phenolics and the antioxidant activities of vegetables with different levels of juices (50μl, 100μl and 200μl) showed positive but not significant correlations (R = 0.41 - 0.55). Some vegetables such as carrots and tomatoes show the highest relative antioxidant

  3. Phenolic profile and antioxidant activities of olive mill wastewater.

    Science.gov (United States)

    El-Abbassi, Abdelilah; Kiai, Hajar; Hafidi, Abdellatif

    2012-05-01

    Olive trees play an important role in the Moroccan agro-economy, providing both employment and export revenue. However, the olive oil industry generates large amounts of wastes and wastewaters. The disposal of these polluting by-products is a significant environmental problem that needs an adequate solution. On one hand, the phytotoxic and antimicrobial effects of olive mill wastewaters are mainly due to their phenolic content. The hydrophilic character of the polyphenols results in the major proportion of natural phenols being separated into the water phase during the olive processing. On other hand, the health benefits arising from a diet containing olive oil have been attributed to its richness in phenolic compounds that act as natural antioxidants and are thought to contribute to the prevention of heart diseases and cancers. Olive mill wastewater (OMW) samples have been analysed in terms of their phenolic constituents and antioxidant activities. The total phenolic content, flavonoids, flavanols, and proanthocyanidins were determined. The antioxidant and radical scavenging activity of phenolic extracts and microfiltred samples was evaluated using different tests (iron(II) chelating activity, total antioxidant capacity, DPPH assays and lipid peroxidation test). The obtained results reveal the considerable antioxidant capacity of the OMW, that can be considered as an inexpensive potential source of high added value powerful natural antioxidants comparable to some synthetic antioxidants commonly used in the food industry. Copyright © 2011 Elsevier Ltd. All rights reserved.

  4. Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

    Directory of Open Access Journals (Sweden)

    Krimat Soumia

    2014-06-01

    Full Text Available Objective: To evaluate the antioxidant and antimicrobial activity of methanolic extract extracts of selected Algerian medicinal plants. Methods: Antioxidant activity of extracts was evaluated in terms of radical scavenging potential (2,2-diphenyl-1-picrylhydrazyl and β-carotene bleaching assay. Total phenolic contents and flavonoid contents were also measured. Antimicrobial activity of these plants was examined against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Results: The values of IC50 ranged from 4.30 μg/mL to 486.6 μg/mL for the DPPH method, while total antioxidant activity using β-carotene/linoleic acid bleaching assay ranged from 17.03% to 86.13%. It was found that Pistacia lentiscus showed the highest antioxidant capacities using DPPH assay (IC50=4.30 μg/mL, while Populus trimula, Origanum glandulosum, Centaurea calcitrapa, Sysimbrium officinalis and Rhamnus alaternus showed the highest percent of total antioxidant activity in β-carotene/linoleic acid bleaching assay. Total phenolic and flavonoid contents ranged from 3.96 to 259.65 mg GAE/g extract and from 1.13 to 26.84 mg QE/g extract, respectively. The most interesting antimicrobial activity was obtained from Sysimbrium officinalis, Rhamnus alaternus, Origanum glandulosum, Cupressus sempervirens, Pinus halipensis and Centaurea calcitrapa. Conclusions: The results indicated that the plants tested may be potential sources for isolation of natural antioxidant and antimicrobial compounds.

  5. Comparative assessment of antioxidant activity of chicory products

    Directory of Open Access Journals (Sweden)

    Z. A. Badretdinova

    2016-01-01

    Full Text Available There are various methods for determining the antioxidant activity. They are differing by the source of oxidation, oxidized compounds and methods for the measurement of oxidized compounds. Methods for determination of antioxidant activity provide a wide range of results, so the evaluation of the antioxidant activity should be carried out in several ways, and the results should be interpreted with caution. In assessing antioxidant capacity is necessary to consider not only the nature and content of the reducing agent in the test facility, but also the possibility of their mutual influence. The objects of research in this research work were Instant chicory (Leroux, Fried chicory (Leroux, Instant chicory (LLC Favorit, Instant chicory (LLC SlavKofe, Instant chicory with hawthorn (Iceberg Ltd and K, Instant chicory (LLC Flagistom, Instant chicory (Ltd. Around the World, Instant chicory (LLC Beta +, Chicory flour (Leroux, Chicory flour. Antioxidant activity of the products of chicory determined by spectrophotometric (using adrenaline and 2,2 – diphenyl- 1-picrylhydrazyl, and amperometric methods. These results indicate that chicory products possess antioxidant activity but not give fully correlated with each other results. The highest antioxidant activity have fried products from chicory – Instant chicory (LLC SlavKofe and Instant chicory with hawthorn (Iceberg Ltd and K. No fried products from chicory (Chicory flour (Leroux have lower antioxidant activity then fried products from chicory. Because during frying new compounds are formed – chikoreol, which comprises acetic and valeric acid, acrolein and furfural, furfuryl alcohol, diacetyl. Best convergence results provide spectrophotometric methods using epinephrine and 2,2 – diphenyl-1-picrylhydrazyl.

  6. Chemical Composition and Antioxidant Activity of Hedychium Malayanum Essential Oils

    International Nuclear Information System (INIS)

    Mohammed Yahya Abdo; Wan Yaacob Wan Ahmad; Laily Din; Nazlina Ibrahim

    2015-01-01

    The leaves and stems of Hedychium malayanum were hydro distilled using a Clevenger-type apparatus to give 0.038 and 0.009 % oils, respectively. Sixteen and seven compounds which made up 98.4 and 100 % of the respective oils were identified using capillary GC and GC-MS with a DB-5 column. These oils contained high amount of monoterpene hydrocarbons with β-pinene being the main compound found in the leaves (39.1 %) and the stems (46.7 %) followed by α-pinene (22.3 %) and β-caryophyllene (13.7 %) which was found in the leaf oil and 1,8-cineole (17.7 %) and α-pinene (16.9 %) in the stem oil. Antioxidant test by 1,1-diphenyl-2-picrylhydrazyl (DPPH) showed that the leaf oil was a stronger antioxidant (31.1 %) than Trolox (28.4 %), whereas the stem oil was the weakest antioxidant (23.9 %). β-Carotene bleaching (BCB) in linoleic acid showed that both oils were weaker antioxidants than butylated hydroxytoluene (96.8 % inhibition). However, the stem oil (79.8 %) showed a good inhibition whereas the leaf oil possessed a moderate capacity (54.7 %) to prevent the oxidation by linoleic acid. (author)

  7. Antimicrobial and antioxidant activity of lemon balm Kombucha

    Directory of Open Access Journals (Sweden)

    Velićanski Aleksandra S.

    2007-01-01

    Full Text Available Kombucha is a beverage traditionally produced by metabolic activity of yeasts and acetic acid bacteria. The antimicrobial activity of lemon balm kombucha as well as of particular control samples was determined by agar-well diffusion method. Antioxidant activity on stable 1,1-diphenyl-2-picrylhydrazyl radicals of lemon balm kombucha and lemon balm tea was determined by electron spin resonance spectroscopy. Acetic acid, Kombucha samples and heat-denaturated kombucha showed significant antimicrobial activity against bacteria. However, there was no activity against yeasts and moulds. Kombucha showed higher antioxidant activity than tea sample for all applied sample volumes.

  8. Correlations between polyphenolic composition and antioxidant activity of Venetian propolis.

    Science.gov (United States)

    Gregoris, Elena; Stevanato, Roberto

    2010-01-01

    Four propolis samples have been picked up in the Venetian region, from different orography and habitative density areas with the purpose to: (i) evaluate propolis' antioxidant activity, measured by inhibition of lipid peroxidation; (ii) determine the polyphenolic components--flavonoids and caffeic acid derivatives--which give antioxidant activity to propolis; (iii) verify the potential correlations between antioxidant activity, polyphenolic content, that has been determined by Folin-Ciocalteu, enzymatic, DPPH quenching, TEAC-like assays, and spectroscopic characteristics of propolis and (iv) correlate chemical structure and antioxidant efficacy of each of the major components. The possible localization of the lipophylic components of propolis into the phospholipidic bilayer by thermal analysis (DSC) and spin label EPR techniques has also been investigated. Copyright 2009 Elsevier Ltd. All rights reserved.

  9. Antioxidant Activity of Marine Algal Polyphenolic Compounds: A Mechanistic Approach.

    Science.gov (United States)

    Fernando, I P Shanura; Kim, Misook; Son, Kwang-Tae; Jeong, Yoonhwa; Jeon, You-Jin

    2016-07-01

    Polyphenolic compounds isolated from marine algae exhibit a broad spectrum of beneficial biological properties, including antioxidant, anticancer, antimicrobial, anti-inflammatory, and antidiabetic activities, along with several other bioactivities centered on their antioxidant properties. Consequently, polyphenolic compounds are increasingly being investigated for their potential use in food, cosmetic, and pharmaceutical applications. The antioxidant activities of these compounds have been explored widely through experimental studies. Nonetheless, a theoretical understanding of the structural and electronic properties could broaden research perspectives, leading to the identification and synthesis of efficient structural analogs with prophylactic uses. This review briefly summarizes the current state of knowledge regarding antioxidant polyphenolic compounds in marine algae with an attempt to describe the structure-activity relationship.

  10. Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms.

    Science.gov (United States)

    Zhang, Lan; Hu, Yu; Duan, Xiaoyu; Tang, Tingting; Shen, Yingbin; Hu, Bin; Liu, Aiping; Chen, Hong; Li, Cheng; Liu, Yuntao

    2018-02-16

    Water-soluble polysaccharides were extracted from the caps and stipes of thirteen boletus mushrooms representing five different species collected in Southwest China. Investigations of their structures and antioxidant activities allowed an evaluation of structure-function relationships. The polysaccharides were composed mainly of the monosaccharides arabinose, xylose, mannose, glucose and galactose. Most samples displayed a broad molecular weight range, with significant differences observed between the molecular weight ranges of the polysaccharides from the caps and the stipes. FT-IR spectral analysis of the polysaccharides revealed that most of polysaccharides from boletus mushrooms (except Boletus edulis) contained a pyranose ring. The antioxidant activities of the polysaccharides in stipes showed a significant correlation with their monosaccharide composition, and were also related to their molecular weight and anomeric configuration. Suillellus luridus collected in Pingwu, Mianyang, Sichuan, China had remarkably superior antioxidant activity and might be developed as a natural antioxidant. Copyright © 2018 Elsevier B.V. All rights reserved.

  11. Antioxidant activity of organic extracts from aqueous infusions of sage.

    Science.gov (United States)

    Matsingou, Triantafillia Christina; Petrakis, Nicolaos; Kapsokefalou, Maria; Salifoglou, Athanasios

    2003-11-05

    The antioxidant activity of aqueous infusions of sage emerges from specific components present in that herb. In an attempt to investigate the chemical nature and properties of these components, four organic solvent extracts from aqueous infusions of sage were examined. HPLC analyses of these extracts led to the separation of a number of components, of which four were identified and quantified through the use of standard compounds of known chromatographic HPLC profiles. These compounds are the diterpenes carnosic acid, carnosol, and rosmanol and the hydroxycinnamic acid caffeic acid. The antioxidant activity and polyphenol content were determined in the four organic solvent extracts and the left-over aqueous fraction. Both polyphenolic and nonpolyphenolic substances present in the extracts arise as significant contributors to the observed antioxidant activity of the derived extracts and thus sage itself. In this sense, they reflect the antioxidant potential of the aqueous infusions of sage toward reactive oxygen species generated through variable mechanisms of iron-promoted oxidative processes.

  12. Synthesis and antioxidant activity of star-shape phenolic antioxidants catalyzed by acidic nanocatalyst based on reduced graphene oxide.

    Science.gov (United States)

    Golestanzadeh, Mohsen; Naeimi, Hossein; Zahraie, Zohreh

    2017-02-01

    Phenolic antioxidants play important role in prevention of oxidation in different industrials. The research objective in the current study was synthesis and evaluate of antioxidant activity of star-shape phenolic antioxidants. The synthetic compounds were prepared in the presence of sulfonated reduced graphene oxide. The antioxidant activity of synthesized compounds was investigated by spectrophotometrically method according to the DPPH assay. Overall, these compounds are potentially important antioxidant and also to limit activity of reactive oxygen species. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. The TEAC antioxidant activity of 4-hydroxybenzoates

    NARCIS (Netherlands)

    Tyrakowska, B.; Soffers, A.E.M.F.; Szymusiak, H.; Boeren, S.; Boersma, M.G.; Lemanska, J.; Vervoort, J.; Rietjens, I.M.C.M.

    1999-01-01

    The influence of pH, intrinsic electron donating capacity, and intrinsic hydrogen atom donating capacity on the antioxidant potential of series of hydroxy and fluorine substituted 4-hydroxybenzoates was investigated experimentally and also on the basis of computer calculations. The pH-dependent

  14. Proximate, mineral composition, antioxidant activity, and total ...

    African Journals Online (AJOL)

    ... of protein supplement, valuable minerals and dietary antioxidants. They can serve as scavengers of free radicals reported to be associated with occurrence of chronic and degenerative diseases such as cardiovascular diseases. Keywords: Peppers, phytochemicals, Nutritional composition, Free radicals, Diseases ...

  15. Antioxidant activities of ethanolic extracts of four Tunisian olive varieties

    Directory of Open Access Journals (Sweden)

    majda Elkateb

    2015-12-01

    Full Text Available Olive leaves extracts of four olive varieties from Tunisian origin: Chitoui, Chimlali, Sahli and Zarrazi were studied for their phenolic contents, antioxidant activities and their effect on corn oil stability. The amounts of total polyphenolics, flavonoids and proanthocyanidins were determined by spectrophotometry. The antioxidant activities of extracts were evaluated and compared with butylated hydroxytoluene (BHT using a             β-carotene bleaching assay and a 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging method. Chimlali ethanol extract showed the highest total phenolics, flavonoids and proanthocyanidins contents expressed respectively in gallic acid, in quercetin  and catechin equivalent per gram of dried matter 245.5; 9.12 and 40.92. Chimlali ethanol extract showed the highest antioxidant activity coefficient (AAC of 863.636 at 200 mg/ml by the β-carotene bleaching method and the highest scavenging activity of 7.5 µg.mL-1 at 0.1 mg/mL by the DPPH method. The obtained results showed that the variety Chimlali is the richest in phenolic compounds, flavonoids, and proanthocyanidins and  the most potent of antioxidant activity. Analysis of the oxidation results of corn oil, heated to 110°C and during frying, showed a significant antioxidant effect of ethanolic olive leaves extracts relative to the BHT. Olive leaves extracts from varieties Chimlali and Sahli showed the highest antioxidant activity. The synthetic antioxidant BHT exhibits a lower antioxidant power than the extracts from varieties Sahli and Chimlali but is comparable to other varieties. The results suggest the introduction of olive leaves extracts in food preparation, both during storage or heating food, for better preservation.

  16. Effect of Freeze-Drying on the Antioxidant Compounds and Antioxidant Activity of Selected Tropical Fruits

    Directory of Open Access Journals (Sweden)

    Muhammad Redzuan Hairuddin

    2011-07-01

    Full Text Available The effects of freeze-drying on antioxidant compounds and antioxidant activity of five tropical fruits, namely starfruit (Averrhoa carambola L., mango (Mangifera indica L., papaya (Carica papaya L., muskmelon (Cucumis melo L., and watermelon Citruluss lanatus (Thunb. were investigated. Significant (p < 0.05 differences, for the amounts of total phenolic compounds (TPC, were found between the fresh and freeze-dried fruit samples, except muskmelon. There was no significant (p > 0.05 change, however, observed in the ascorbic acid content of the fresh and freeze-dried fruits. Similarly, freeze-drying did not exert any considerable effect on β-carotene concentration of fruits, except for mango and watermelon, where significantly (p < 0.05 higher levels were detected in the fresh samples. The results of DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging and reducing power assays revealed that fresh samples of starfruit and mango had relatively higher antioxidant activity. In case of linoleic acid peroxidation inhibition measurement, a significant (p < 0.05 but random variation was recorded between the fresh and freeze-dried fruits. Overall, in comparison to β-carotene and ascorbic acid, a good correlation was established between the result of TPC and antioxidant assays, indicating that phenolics might have been the dominant compounds contributing towards the antioxidant activity of the fruits tested.

  17. In vitro antioxidant activities of edible artichoke (Cynara scolymus L.) and effect on biomarkers of antioxidants in rats

    DEFF Research Database (Denmark)

    Jimenez-Escrig, A.; Dragsted, Lars Ove; Daneshvar, Bahram

    2003-01-01

    Artichoke (Cynara scolymus L.), an edible vegetable from the Mediterranean area, is a good source of natural antioxidants such as vitamin C, hydroxycinnamic acids, and flavones. The antioxidant activity of aqueous-organic extracts of artichoke were determined using three methods: (a) free radical 2......,2-diphenyl-1-picrylhydrazyl (DPPH.) scavenging, (b) ferric-reducing antioxidant power (FRAP), and...

  18. Amazonian palm Oenocarpus bataua ("patawa"): chemical and biological antioxidant activity--phytochemical composition.

    Science.gov (United States)

    Rezaire, A; Robinson, J-C; Bereau, D; Verbaere, A; Sommerer, N; Khan, M K; Durand, P; Prost, E; Fils-Lycaon, B

    2014-04-15

    In French Guiana, "diversity" within the Palm family is obvious since more than 75 species have been identified. Oenocarpus bataua Mart., called "patawa" is well known for its culinary uses whereas literature on its phytochemical composition and biological properties remains poor. This work deals with determining the antioxidant activity of this palm fruit and its polyphenol composition; Euterpe oleracea (açai) used as a reference. It turned out that patawa had a stronger antioxidant activity than açai in TEAC and FRAP tests. A similar activity was observed by DPPH assay whereas in ORAC and KRL tests, that açai showed an antioxidant activity respectively 2.6 and 1.5 fold higher than patawa. Polyphenolic composition, determined by UPLC/MS(n), would imply the presence of anthocyanins, condensed tannins, stilbenes and phenolic acids, well known for their biological activities. These results present patawa fruit as a new amazonian resource for cosmetics, food and pharmaceuticals purposes. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. Chemical characterization and antioxidant activity of Eryngium campestre L., Apiaceae from Kosovo

    Directory of Open Access Journals (Sweden)

    Flurim Nebija

    2009-12-01

    Full Text Available This study is outlined to define the chemical composition and in vitro antioxidant activity of the extracts of aerial part and root of Eryngium campestre L. (Apiaceae from Kosovo. Analysis of the chemical composition include determination of total ash, ash insoluble in hydrochloric acid, loss on drying and the content of water extract, as well as determination of flavonoids in aerial part and hemolytic activity of the root. The mineral composition (Zn, Fe, Cu, Mn, Ni, K, Co, Pb, Cd and Cr in aerial parts and root has been studied using atomic absorption spectroscopy (AAS and ETAAS. Different part of E. campestre accumulate different amounts of investigated minerals. Antioxidant activity was determined by four various testing systems: DPPH assay, inhibition of production of hydroxyl radical, β-carotenebleaching assay, and inhibition of lipid peroxidation (TBA test. In DPPH system, ethanol extract of root of E. campestre exhibited higher radical-scavenging activity (IC50 = 0.72 mg ml-1 compared to the extract of the aerial part (IC50 = 1.14 mg ml-1. On the other hand, aerial part ethanol extract has exhibited stronger inhibition capacity on the production of hydroxyl radical in deoxyribose system than the root extract (50% and 45%, respectively. However, both ethanol extracts of E. campestre exhibited low antioxidant activity in β-carotenebleaching assay as well as, low capacity for inhibition of spontaneous lipid peroxidation in rat liver homogenate.

  20. Comparison in antioxidant and antitumor activities of pine polyphenols and its seven biotransformation extracts by fungi

    OpenAIRE

    Hui Li; Zhenyu Wang

    2017-01-01

    Microbial transformation can strengthen the antioxidant and antitumor activities of polyphenols. Polyphenols contents, antioxidant and antitumor activities of pine polyphenols and its biotransformation extracts by Aspergillus niger, Aspergillus oryzae, Aspergillus carbonarius, Aspergillus candidus, Trichodermas viride, Mucor wutungkiao and Rhizopus sp were studied. Significant differences were noted in antioxidant and antitumor activities. The highest antioxidant activities in Trolox equivale...

  1. Antioxidants

    Science.gov (United States)

    Antioxidants are man-made or natural substances that may prevent or delay some types of cell damage. Antioxidants are found in many foods, including fruits and ... are also available as dietary supplements. Examples of antioxidants include Beta-carotene Lutein Lycopene Selenium Vitamin A ...

  2. Antioxidative and anticholinesterase activity of Cyphomandra betacea fruit.

    Science.gov (United States)

    Ali Hassan, Siti Hawa; Abu Bakar, Mohd Fadzelly

    2013-01-01

    Cyphomandra betacea is one of the underutilized fruits which can be found in tropical and subtropical countries. This study was conducted to determine the antioxidant activity and phytochemical contents in different parts (i.e., flesh and peel) of the fruits. Antioxidants were analyzed using DPPH and ABTS free radical scavenging assays as well as FRAP assay. Anticholinesterase activity was determined using enzymatic assay using acetyl cholinesterase enzyme. For 80% methanol extract, the peel of the fruit displayed higher antioxidant activity in both FRAP and ABTS free radical scavenging assays while the flesh displayed higher antioxidant activity in the DPPH assay. Total phenolic and total flavonoid content were higher in the peel with the values of 4.89 ± 0.04 mg gallic acid equivalent (GAE)/g and 3.36 ± 0.01 mg rutin equivalent (RU)/g, respectively. Total anthocyanin and carotenoid content were higher in the flesh of the fruit with the values of 4.15 ± 0.04 mg/100 g and 25.13 ± 0.35 mg/100 g. The anticholinesterase was also higher in the peel of C. betacea. The same trends of phytochemicals, antioxidant, and anticholinesterase were also observed in the distilled water extracts. These findings suggested that C. betacea has a potential as natural antioxidant-rich nutraceutical products.

  3. Antioxidative and Anticholinesterase Activity of Cyphomandra betacea Fruit

    Directory of Open Access Journals (Sweden)

    Siti Hawa Ali Hassan

    2013-01-01

    Full Text Available Cyphomandra betacea is one of the underutilized fruits which can be found in tropical and subtropical countries. This study was conducted to determine the antioxidant activity and phytochemical contents in different parts (i.e., flesh and peel of the fruits. Antioxidants were analyzed using DPPH and ABTS free radical scavenging assays as well as FRAP assay. Anticholinesterase activity was determined using enzymatic assay using acetyl cholinesterase enzyme. For 80% methanol extract, the peel of the fruit displayed higher antioxidant activity in both FRAP and ABTS free radical scavenging assays while the flesh displayed higher antioxidant activity in the DPPH assay. Total phenolic and total flavonoid content were higher in the peel with the values of 4.89 ± 0.04 mg gallic acid equivalent (GAE/g and 3.36 ± 0.01 mg rutin equivalent (RU/g, respectively. Total anthocyanin and carotenoid content were higher in the flesh of the fruit with the values of 4.15 ± 0.04 mg/100 g and 25.13 ± 0.35 mg/100 g. The anticholinesterase was also higher in the peel of C. betacea. The same trends of phytochemicals, antioxidant, and anticholinesterase were also observed in the distilled water extracts. These findings suggested that C. betacea has a potential as natural antioxidant-rich nutraceutical products.

  4. Antioxidant Activity in the Blood and Testes of the Mottled Brown ...

    African Journals Online (AJOL)

    IJAAAR

    homogenate fluids were centrifuged separately to harvest the supernatants and analysed for the antioxidant indices. Statistical comparisons were made between the serum and testicular antioxidant indices. Result showed that the activities of catalase, superoxide dismutase, glutathione peroxidase and total antioxidant.

  5. Mechanical stretch endows mesenchymal stem cells stronger angiogenic and anti-apoptotic capacities via NFκB activation

    International Nuclear Information System (INIS)

    Zhu, Zhuoli; Gan, Xueqi; Fan, Hongyi; Yu, Haiyang

    2015-01-01

    Mesenchymal stem cells (MSCs) have been broadly used for tissue regeneration and repair due to their broad differentiation potential and potent paracrine properties such as angiogenic capacity. Strategies to increase their survival rate after transplantation and the angiogenic ability are of priority for the utility of MSCs. In this study, we found that mechanical stretch (10% extension, 30 cycles/min cyclic stretch) preconditioning increase the angiogenic capacity via VEGFA induction. In addition, mechanical stretch also increases the survival rate of mesenchymal stem cells under nutrients deprivation. Consistent with the increase VEGFA expression and resistance to apoptosis, nuclear localization of NFκB activity p65 increased upon mechanical stretch. Inhibition of NFκB activity by BAY 11-708 blocks the pro-angiogenesis and anti-apoptosis function of mechanical stretch. Taken together, our findings here raise the possibility that mechanical stretch preconditioning might enhance the therapeutic efficacy of mesenchymal stem cells. - Highlights: • Mechanical stretch increases the angiogenic capacity via VEGFA induction in MSCs. • Mechanical stretch increases the survival rate of MSCs under nutrients deprivation. • Mechanical stretch manipulates MSCs via the activation of NFκB.

  6. Mechanical stretch endows mesenchymal stem cells stronger angiogenic and anti-apoptotic capacities via NFκB activation

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Zhuoli; Gan, Xueqi [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041 (China); Fan, Hongyi [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041 (China); Department of Applied Mechanics, College of Architecture and Environment, Sichuan University, Chengdu 610065 (China); Yu, Haiyang, E-mail: yhyang6812@foxmail.com [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041 (China)

    2015-12-25

    Mesenchymal stem cells (MSCs) have been broadly used for tissue regeneration and repair due to their broad differentiation potential and potent paracrine properties such as angiogenic capacity. Strategies to increase their survival rate after transplantation and the angiogenic ability are of priority for the utility of MSCs. In this study, we found that mechanical stretch (10% extension, 30 cycles/min cyclic stretch) preconditioning increase the angiogenic capacity via VEGFA induction. In addition, mechanical stretch also increases the survival rate of mesenchymal stem cells under nutrients deprivation. Consistent with the increase VEGFA expression and resistance to apoptosis, nuclear localization of NFκB activity p65 increased upon mechanical stretch. Inhibition of NFκB activity by BAY 11-708 blocks the pro-angiogenesis and anti-apoptosis function of mechanical stretch. Taken together, our findings here raise the possibility that mechanical stretch preconditioning might enhance the therapeutic efficacy of mesenchymal stem cells. - Highlights: • Mechanical stretch increases the angiogenic capacity via VEGFA induction in MSCs. • Mechanical stretch increases the survival rate of MSCs under nutrients deprivation. • Mechanical stretch manipulates MSCs via the activation of NFκB.

  7. Impact of functional properties and release kinetics on antioxidant activity of biopolymer active films and coatings.

    Science.gov (United States)

    Benbettaïeb, Nasreddine; Tanner, Cadhla; Cayot, Philippe; Karbowiak, Thomas; Debeaufort, Frédéric

    2018-03-01

    This work deals with the study of the release kinetics of some natural antioxidants (ferulic acid, caffeic acid and tyrosol) from chitosan-fish gelatin edible films immersed ethanol at 96%, as well as the kinetics of their antioxidant activity using the DPPH assay. The aim was to determine how film functional properties influence the release kinetic and antioxidant activity. The addition of antioxidants to chitosan-fish gelatin matrix decreased the water vapour permeability by more than 30%. The tensile strength (TS) increased up to 50% after the incorporation of antioxidants. Some molecular interactions between polymer chains and antioxidants were confirmed by FTIR where spectra displayed a shift of the amide-III peak. Films containing caffeic acid or a caffeic-ferulic acid mixture exhibited the highest radical scavenging activity, leading to a 90% antioxidant activity at equilibrium but the release rate controlled the efficacy of the system. Copyright © 2017 Elsevier Ltd. All rights reserved.

  8. Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds

    Directory of Open Access Journals (Sweden)

    A. Tariq

    2015-01-01

    Full Text Available In the present study ethanol and water extracts of 15 seaweeds, Dictyota dichotoma var. velutricata, Dictyota indica, Iyengaria stellata, Padina pavonia, Sargassum swartzii, Sargassum variegatum, Stoechospermum marginatum, Stokeyia indica, Jolyna laminarioides, Caulerpa taxifolia, Halimeda tuna, Ulva fasciata, Ulva lactuca, Solieria robusta, and Melanothamnus afaqhusainii, were evaluated for their antioxidant potential by ABTS or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid, superoxide and total antioxidant capacity (TAC assays.  The activity was concentration dependent and the variation in antioxidant potential was also observed by different assays in both extracts.  Ethanol extract of D. dichotoma var. velutricata, D. indica and S. marginatum demonstrated highest activity by TAC assay.  The antioxidant potential in organic solvent fractions of seaweeds namely P. pavonia, S. swartzii, S. marginatum and M. afaqhusainii was also determined and chloroform fraction of all the four seaweeds showed highest activity by superoxide assay.  Antioxidant activity of extracted fractions of polysaccharides from S. indica, C. taxifolia and D. dichotoma var. velutricata was also evaluated by superoxide method.  Polysaccharide fractions of S. indica obtained from HCl (at 700C and room temperature and water extract demonstrated highest activity respectively.  All the polysaccharide fractions of C. taxifolia showed excellent activity except CaClF70°C. Polysaccharide fractions of D. dichotoma var. velutricata also exhibited very good activity.

  9. Chemical Composition, Antifungal and Antioxidant Activities of Hedyosmum brasiliense Mart. ex Miq. (Chloranthaceae) Essential Oils

    Science.gov (United States)

    Murakami, Cynthia; Cordeiro, Inês; Young, Maria Cláudia M.

    2017-01-01

    Background: Hedyosmum brasiliense Mart. ex Miq. (Chloranthaceae) is a dioecious shrub popularly used in Brazil to treat foot fungi and rheumatism. This work investigated the chemical composition, antifungal, and antioxidant activities of flowers and leaves of H. brasiliense essential oils; Methods: H. brasiliense male and female flowers and leaves were collected at Ilha do Cardoso (São Paulo) and the essential oils were extracted by hydrodistillation and analyzed by GC/MS and their similarity compared by Principal Component Analysis. Antifungal activity was performed by bioautography and antioxidant potential by 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) free radical scavenging and β-carotene/linoleic acid system; Results: The major compounds for all oils were sabinene, curzerene, and carotol, but some differences in their chemical composition were discriminated by Principal Component Analysis (PCA) analysis. Bioautography showed two antifungal bands at Rf’s 0.67 and 0.12 in all samples, the first one was identified as curzerene. The oils presented stronger antioxidant potential in β-carotene/linoleic acid bioassay, with IC50’s from 80 to 180 μg/mL, than in DPPH assay, with IC50’s from 2516.18 to 3783.49 μg/mL; Conclusions: These results suggested that curzerene might be responsible for the antifungal activity of H. brasiliense essential oils. Besides, these essential oils exhibited potential to prevent lipoperoxidation, but they have a weak radical scavenger activity. PMID:28930269

  10. Antioxidant Activity of Hawaiian Marine Algae

    OpenAIRE

    Kelman, Dovi; Posner, Ellen Kromkowski; McDermid, Karla J.; Tabandera, Nicole K.; Wright, Patrick R.; Wright, Anthony D.

    2012-01-01

    Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that cou...

  11. The Antioxidant Activity and Their Major Antioxidant Compounds from Acanthopanax senticosus and A. koreanum

    Directory of Open Access Journals (Sweden)

    Young-Hyun Kim

    2015-07-01

    Full Text Available The antioxidant activity and chlorogenic acid and caffeic acid contents were investigated from different parts of Acanthopanax senticosus and A. koreanum. Antioxidant activity was assessed by various in vitro assays such as DPPH, ABTS, FRAP, reducing power assays and ORAC, and the chlorogenic acid and caffeic acid were validated by HPLC chromatography. Among the various extracts, the fruit extracts of A. senticosus and A. koreanum exhibited strongest antioxidant activities including ABTS, FRAP, reducing power and ORAC, however, strongest DPPH radical scavenging activity was observed from the leaf extract of A. senticosus. In addition, the antioxidant activities of various extracts were correlated with total phenolic and proanthocyanidin contents. The major phenolic contents from various parts of these plants observed that leaf extract of A. senticosus expressed higher levels of chlorogenic acid (14.86 mg/dry weigh g and caffeic acid (3.09 mg/dry weigh g than other parts. Therefore, these results suggest that the leaf of A. senticosus may be an excellent natural source for functional foods and pharmaceutical agents, and the validated method was useful for the quality control of A. senticosus.

  12. Antioxidant activities of various fruits and vegetables produced in Taiwan.

    Science.gov (United States)

    Huang, Hui-Yu; Chang, Chen-Kang; Tso, Tim K; Huang, Ju-Jen; Chang, Wei-Wei; Tsai, Ying-Chieh

    2004-08-01

    Fruits and vegetables have been known to contain a variety of antioxidant components. It has been suggested that antioxidants may protect biomolecules from oxidative damage and therefore be associated with reduced risks of cardiovascular disease and certain cancer. The antioxidant abilities of various parts of eight common fruits and vegetables produced in Taiwan were investigated, including tomato, guava, squash, tangerine, wax gourd, pineapple, chayote, and eggplant. Squash, wax gourd, tomato, and guava seeds showed the highest antioxidant activities in thiobarbituric acid assay. Wax guard and squash seeds showed the highest antioxidant activities in iodometric assay. At the level of 1 g fresh sample, low-density lipoprotein peroxidation was inhibited by at least 90% by tomato meat, guava meat, squash seed, wax gourd meat, core, and seed, and eggplant skin. The total phenolic content was significantly correlated with antioxidant activities measured by thiobarbituric acid (r=0.715, P<0.01) and iodometric (r=0.749, P<0.01) assays. The results of this study could be used for development of merchandise with potential health benefits from agricultural products.

  13. Antioxidant Activity in Extracts of 27 Indigenous Taiwanese Vegetables

    Directory of Open Access Journals (Sweden)

    Pi-Yu Chao

    2014-05-01

    Full Text Available The objectives of this study were to identify the antioxidants and antioxidant axtivity in 27 of Taiwan’s indigenous vegetables. Lycium chinense (Lc, Lactuca indica (Li, and Perilla ocymoides (Po contained abundant quercetin (Que, while Artemisia lactiflora (Al and Gynura bicolor (Gb were rich in morin and kaempferol, respectively. Additionally, Nymphoides cristata (Nc and Sechium edule (Se-yellow had significantly higher levels of myricetin (Myr than other tested samples. Cyanidin (Cyan and malvidin (Mal were abundant in Gb, Abelmoschus esculentus Moench (Abe, Po, Anisogonium esculentum (Retz. Presl (Ane, Ipomoea batatas (Ib-purple, and Hemerocallis fulva (Hf-bright orange. Relatively high levels of Trolox equivalent antioxidant capacity (TEAC, oxygen radical absorption capacity (ORAC, and 1,1-diphenyl-2-picryl-hydrazyl (DPPH radical scavenger were generated from extracts of Toona sinensis (Ts and Po. Significant and positive correlations between antioxidant activity and polyphenols, anthocyanidins, Que, Myr, and morin were observed, indicating that these phytochemicals were some of the main components responsible for the antioxidant activity of tested plants. The much higher antioxidant activity of Po, Ts, and Ib (purple leaf may be related to their higher Cyan, Que, and polyphenol content.

  14. Antioxidant Activity in Extracts of 27 Indigenous Taiwanese Vegetables

    Science.gov (United States)

    Chao, Pi-Yu; Lin, Su-Yi; Lin, Kuan-Hung; Liu, Yu-Fen; Hsu, Ju-Ing; Yang, Chi-Ming; Lai, Jun-You

    2014-01-01

    The objectives of this study were to identify the antioxidants and antioxidant axtivity in 27 of Taiwan’s indigenous vegetables. Lycium chinense (Lc), Lactuca indica (Li), and Perilla ocymoides (Po) contained abundant quercetin (Que), while Artemisia lactiflora (Al) and Gynura bicolor (Gb) were rich in morin and kaempferol, respectively. Additionally, Nymphoides cristata (Nc) and Sechium edule (Se)-yellow had significantly higher levels of myricetin (Myr) than other tested samples. Cyanidin (Cyan) and malvidin (Mal) were abundant in Gb, Abelmoschus esculentus Moench (Abe), Po, Anisogonium esculentum (Retz.) Presl (Ane), Ipomoea batatas (Ib)-purple, and Hemerocallis fulva (Hf)-bright orange. Relatively high levels of Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorption capacity (ORAC), and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenger were generated from extracts of Toona sinensis (Ts) and Po. Significant and positive correlations between antioxidant activity and polyphenols, anthocyanidins, Que, Myr, and morin were observed, indicating that these phytochemicals were some of the main components responsible for the antioxidant activity of tested plants. The much higher antioxidant activity of Po, Ts, and Ib (purple leaf) may be related to their higher Cyan, Que, and polyphenol content. PMID:24858497

  15. Antioxidant activity in extracts of 27 indigenous Taiwanese vegetables.

    Science.gov (United States)

    Chao, Pi-Yu; Lin, Su-Yi; Lin, Kuan-Hung; Liu, Yu-Fen; Hsu, Ju-Ing; Yang, Chi-Ming; Lai, Jun-You

    2014-05-23

    The objectives of this study were to identify the antioxidants and antioxidant axtivity in 27 of Taiwan's indigenous vegetables. Lycium chinense (Lc), Lactuca indica (Li), and Perilla ocymoides (Po) contained abundant quercetin (Que), while Artemisia lactiflora (Al) and Gynura bicolor (Gb) were rich in morin and kaempferol, respectively. Additionally, Nymphoides cristata (Nc) and Sechium edule (Se)-yellow had significantly higher levels of myricetin (Myr) than other tested samples. Cyanidin (Cyan) and malvidin (Mal) were abundant in Gb, Abelmoschus esculentus Moench (Abe), Po, Anisogonium esculentum (Retz.) Presl (Ane), Ipomoea batatas (Ib)-purple, and Hemerocallis fulva (Hf)-bright orange. Relatively high levels of Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorption capacity (ORAC), and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenger were generated from extracts of Toona sinensis (Ts) and Po. Significant and positive correlations between antioxidant activity and polyphenols, anthocyanidins, Que, Myr, and morin were observed, indicating that these phytochemicals were some of the main components responsible for the antioxidant activity of tested plants. The much higher antioxidant activity of Po, Ts, and Ib (purple leaf) may be related to their higher Cyan, Que, and polyphenol content.

  16. Comparison of Antioxidant Evaluation Assays for Investigating Antioxidative Activity of Gallic Acid and Its Alkyl Esters in Different Food Matrices.

    Science.gov (United States)

    Phonsatta, Natthaporn; Deetae, Pawinee; Luangpituksa, Pairoj; Grajeda-Iglesias, Claudia; Figueroa-Espinoza, Maria Cruz; Le Comte, Jérôme; Villeneuve, Pierre; Decker, Eric A; Visessanguan, Wonnop; Panya, Atikorn

    2017-08-30

    The addition of antioxidants is one of the strategies to inhibit lipid oxidation, a major cause of lipid deterioration in foods leading to rancidity development and nutritional losses. However, several studies have been reported that conventional antioxidant assays, e.g., TPC, ABTS, FRAP, and ORAC could not predict antioxidant performance in several foods. This study aimed to investigate the performance of two recently developed assays, e.g., the conjugated autoxidizable triene (CAT) and the apolar radical-initiated conjugated autoxidizable triene (ApoCAT) assays to predict the antioxidant effectiveness of gallic acid and its esters in selected food models in comparison with the conventional antioxidant assays. The results indicated that the polarities of the antioxidants have a strong impact on antioxidant activities. In addition, different oxidant locations demonstrated by the CAT and ApoCAT assays influenced the overall antioxidant performances of the antioxidants with different polarities. To validate the predictability of the assays, the antioxidative performance of gallic acid and its alkyl esters was investigated in oil-in-water (O/W) emulsions, bulk soybean oils, and roasted peanuts as the lipid food models. The results showed that only the ApoCAT assay could be able to predict the antioxidative performances in O/W emulsions regardless of the antioxidant polarities. This study demonstrated that the relevance of antioxidant assays to food models was strongly dependent on physical similarities between the tested assays and the food structure matrices.

  17. Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract.

    Science.gov (United States)

    Al-Abd, Nazeh M; Mohamed Nor, Zurainee; Mansor, Marzida; Azhar, Fadzly; Hasan, M S; Kassim, Mustafa

    2015-10-24

    The threat posed by drug-resistant pathogens has resulted in the increasing momentum in research and development for effective alternative medications. The antioxidant and antibacterial properties of phytochemical extracts makes them attractive alternative complementary medicines. Therefore, this study evaluated the phytochemical constituents of Melaleuca cajuputi flower and leaf (GF and GL, respectively) extracts and their antioxidant and antibacterial activities. Radical scavenging capacity of the extracts was estimated using 2,2-diphenyl-2-picrylhydrazyl and Fe(2+)-chelating activity. Total antioxidant activity was determined using ferric reducing antioxidant power assay. Well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration assays were used to determine antibacterial activity against eight pathogens, namely Staphylococcus aureus, Escherichia coli, Bacillus cereus, Staphylococcus epidermidis, Salmonella typhimurium, Klebsiella pneumonia, Streptococcus pneumoniae, and Pasteurella multocida. We identified and quantified the phytochemical constituents in methanol extracts using liquid chromatography/mass spectrometry (LC/MS) and gas chromatography (GC)/MS. This study reports the antioxidant and radical scavenging activity of M. cajuputi methanolic extracts. The GF extract showed better efficacy than that of the GL extract. The total phenolic contents were higher in the flower extract than they were in the leaf extract (0.55 ± 0.05 and 0.37 ± 0.05 gallic acid equivalent per mg extract dry weight, respectively). As expected, the percentage radical inhibition by GF was higher than that by the GL extract (81 and 75 %, respectively). A similar trend was observed in Fe(2+)-chelating activity and β-carotene bleaching tests. The antibacterial assay of the extracts revealed no inhibition zones with the Gram-negative bacteria tested. However, the extracts demonstrated activity against B. cereus, S. aureus, and S. epidermidis. In

  18. Synthesis, antimicrobial and antioxidative activity of some new isatin derivatives

    Directory of Open Access Journals (Sweden)

    Šekularac Gavrilo M.

    2014-01-01

    Full Text Available The isatin derivatives, Schiff bases, were synthesized by the reaction of isatin and various substituted primary amines and characterized by several spectroscopic methods. Investigation of the antimicrobial activity of the synthesized compounds was performed by the agar dilution method, against different strains of bacteria and one fungi. The antioxidative activity of the synthesized compounds was also determined. Some of the compounds have shown the significant activity against the selected strains of microorganisms and the antioxidative activity. [Projekat Ministarstva nauke Republike Srbije, br. 172013 i III 46010

  19. Evaluation of Antioxidant and Anti-neuroinflammatory Activities of ...

    African Journals Online (AJOL)

    ... NF-κB activation in LPS-stimulated BV-2 cells. Conclusion: HFE can be considered as a useful therapeutic and preventive approach for the treatment of neurodegenerative diseases and oxidative stress-related diseases. Keywords: Hizikia fusiforme, Antioxidant activity, Anti-neuroinflammation activity, Inducible nitric oxide ...

  20. Antioxidative and antibacterial activity of the methanol extract of ...

    African Journals Online (AJOL)

    Antioxidative and antibacterial activity of the methanol extract of Artemisia anomala S. Moore. ... activity against all five test bacteria and most activity against Gram-negative ones. The minimum inhibitory concentration (MIC) against Proteus vulgaris, Escherichia coli and Salmonella typhi were 1.5, 4.5, 3.9 mg/ml, respectively.

  1. Antioxidant activity relationship of phenolic compounds in Hypericum perforatum L.

    Directory of Open Access Journals (Sweden)

    Petrović Slobodan S

    2011-06-01

    Full Text Available Abstract Background The St John's Wort (Hypericum perforatum; Clusiaceae has been used in traditional and modern medicine for a long time due to its high content of biologically active phenolics. The purpose of this work was to develop a method for their fractionation and identification, and to determine the most active antioxidant compounds in plant extract. Results An LC-MS method which enables fast qualitative and semiquantitative analysis was developed. The composition determined is in agreement with the previous results, where 6 flavonoids, 4 naphthodianthrones and 4 phloroglucinols have been identified. Significant antioxidant activity was determined for most of the fractions by DPPH assay (the lowest IC50 of 0.52 μg/ml, NO scavenging (6.11 μg/ml, superoxide scavenging (1.86 μg/ml, lipid peroxidation (0.0079 μg/ml and FRAP (the highest reduction capacity of 104 mg Fe equivalents/g assays. Conclusion LC-MS technique has been successfully applied for a quick separation and identification of the major components of H. perforatum fractions. Majority of the fractions analyzed have expressed a very high antioxidative activity when compared to synthetic antioxidants. The antioxidant activity could be attributed to flavonoids and phenolic acids, while phloroglucinols and naphthodianthrones showed no significant activity. It is demonstrated that it is possible to obtain, by fractionation, H. perforatum preparations with significantly increased phloroglucinols-to-naphthodianthrones ratio (up to 95:5.

  2. Nano-zirconia - Evaluation of its antioxidant and anticancer activity.

    Science.gov (United States)

    Balaji, Siripireddy; Mandal, Badal Kumar; Ranjan, Shivendu; Dasgupta, Nandita; Chidambaram, Ramalingam

    2017-05-01

    Bioactivity of nanomaterials largely depends on its size, shape and crystalline nature. In this work, the smaller sized spherical shaped nano-zirconia (ZrO 2 NPs) (of ~9 to 11nm) was fabricated and studied its biological activity especially antioxidant and cytotoxicity against human colon carcinoma (HCT-116) and human lung carcinoma (A-549) cell lines. To have its real applications in biological aspects readily available Eucalyptus globulus (E. globulus) leaf extract was used as an effective capping and reducing agent for its synthesis. The prepared ZrO 2 NPs was characterized by using different sophisticated instrumentations such as UV-visible spectrophotometer, XRD, FTIR, TEM, SAED, EDX, DLS and fluorescence spectroscopy. Cellular mitochondrial activity i.e. cell viability was measured by MTT assay and anti-oxidant activity was determined by DPPH assay. The smaller sized ZrO 2 NPs showed strong antioxidant activity as well as cytotoxicity on human cancer cell lines. Comparative cytotoxic studies were conducted on human cancerous cell lines using different techniques. Results confirmed the efficient anti-cancer activities of the fabricated ZrO 2 NPs towards the tested cell lines as well as efficient anti-oxidant activity. This is the first study in which E. globulus leaf extract was used to synthesize smaller spherical shaped ZrO 2 NPs for improved bioactivity i.e. antioxidant and cytotoxicity. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Study on the antioxidant and antitumoral activity of propolis

    Directory of Open Access Journals (Sweden)

    Elisa del Río del Rosal

    Full Text Available ABSTRACT Introduction: Propolis is the substance that protects the hive, a resin of complex and viscous composition bees use in the repair and protection of the hive. The material from which propolis arises are the resins, shoots and petioles of the leaves of different plants, so it has a very complex chemical composition that varies depending on the flora of the bees collection. It offers an antimicrobial, anti-inflammatory capacity related to its antioxidant, immunomodulatory power, among others. Aims: In this work, antioxidant and antitumoral activities of different propolis collected from different areas of the province of Malaga, comparing them with one from the Bohemian region to the south of the Czech Republic are studied. Material and methods: Antioxidant activity was determined according to the ABTS+/S2O8K2 method. In addition, the quantity of total proteins from the nitrogen content and subsequently the cytotoxicity and antitumoral activity of the propolis of Puerto de la Torre, north of Malaga, are measured according to the 3-(4,5-dimetiltiazol-2-il-2,5-diphenyl tetrazolium bromide method. Results: It was observed that propolis has high antioxidant activity, although it has a lower amount of proteins. Propolis has high toxicity and higher antitumoral activity against colon cancer than leukemia. Discussion: With all these data, it can be concluded that propolis offers different activities of interest, for the food and cosmetic industry, among which the high antioxidant and antitumoral capacity.

  4. Comparative study on the antioxidant activity of peptides from pearl oyster ( Pinctada martensii) mantle type V collagen and tilapia ( Oreochromis niloticus) scale type I collagen

    Science.gov (United States)

    Xia, Guanghua; Zhang, Xueying; Dong, Zhenghua; Shen, Xuanri

    2017-12-01

    In this study, Pearl oyster mantle type V collagen (POMC) and tilapia scale type I collagen (TSC) were extracted and hydrolyzed by various proteases in order to obtain peptides. The antioxidant activity of the peptides was investigated by DPPH, hydroxyl radical scavenging experiments and a dynamic digestion model in vitro. The results show that there are significant differences in amino acid composition between POMC and TSC. The collagen peptides obtained from pearl oyster mantle (POMCP) by treating with alkaline protease exhibited higher antioxidant activity than that from tilapia scale (TSCP) treated with papaya protease, and both of them showed greater DPPH and hydroxyl radical scavenging activity than other peptides. After being separated via Sephadex G-25 chromatography, the M1 fraction isolated from POMCP, and the S1 fraction from TSCP with which both had higher molecular weights showed the strongest antioxidant activity than other fractions, and the M1 fraction exhibited stronger antioxidant activity than the S1 fraction in scavenging free-radicals and protecting cells from the oxidation damage. Furthermore, after treating the dynamic digestion system model in vitro, the DPPH and hydroxyl radical scavenging activity of the M1 fraction increased slightly. These results suggest that POMCP exhibits stronger antioxidant activity than TSCP, which means that PMOP may be a good candidate to be a potential natural antioxidant in the food-processing industry.

  5. Antioxidant activity and phenylpropanoids of Phlomis lychnitis L

    DEFF Research Database (Denmark)

    López, Víctor; Jäger, Anna Katharina; Akerreta, Silvia

    2010-01-01

    Phlomis lychnitis L. (Lamiaceae) is consumed as a traditional herbal tea in Spain. The antioxidant-protective effects as well as its phytoconstituents have never been established. The ability of the methanolic extract to protect cells from oxidative stress was evaluated in rat pheochromocytoma...... cells (PC12) using hydrogen peroxide as toxic agent. The viability of PC12 cells pre-treated with the methanolic extract of Phlomis lychnitis, determined by the MTT and LDH assays, was significantly improved at the highest dose (p antioxidant activity was confirmed evaluating the capacity...... of the plant to scavenge ABTS, DPPH, O(2) . (-) radicals and to inhibit XO. Bioassay guided fractionation led to antioxidant compounds. Qualitative HPLC/DAD/ESI/MS analysis reported phenylpropanoids, verbascoside being the major antioxidant constituent....

  6. Antioxidant activity of Egyptian Eucalyptus camaldulensis var. brevirostris leaf extracts.

    Science.gov (United States)

    El-Ghorab, Ahmed H; El-Massry, Khaled F; Marx, Friedhelm; Fadel, Hoda M

    2003-02-01

    Leaves from Eucalvptus camaldulensis var. brevirostris trees, planted in the Nile delta in Egypt, were examined for the antioxidant activity of their nonvolatile compounds. The extracts obtained by ethanol digestion and by supercritical fluid extraction (SFE; CO2 with 15% ethanol) showed the most promising antioxidative activities. In order to identify the most active compounds, both extracts were subjected to a semipreparative reversed-phase HPLC separation, the main fractions were collected, tested for antioxidative activity and analysed by different chromatographical and spectroscopical methods for identification of the most relevant compounds. Gallic and ellagic acid were found to be the prevailing antioxidants in the ethanolic extract. The main two compounds of the SFE extract with antioxidative activity revealed to be flavones. To a high degree of probability they were identified as 5-hydroxy-7,4'-dimethoxy flavone and 5-hydroxy-7,4'-dimethoxy-8-methyl flavone, respectively. The extracts obtained by ethanoldigestion were dried and administered to rats for toxicity evaluation (up to 3 g/kg body weight). No mortality was observed which indicates a very low lethality of the tested extract.

  7. Spectroscopic studies on the antioxidant activity of ellagic acid

    Science.gov (United States)

    Kilic, Ismail; Yeşiloğlu, Yeşim; Bayrak, Yüksel

    2014-09-01

    Ellagic acid (EA, C14H6O8) is a natural dietary polyphenol whose benefits in a variety of diseases shown in epidemiological and experimental studies involve anti-inflammation, anti-proliferation, anti-angiogenesis, anticarcinogenesis and anti-oxidation properties. In vitro radical scavenging and antioxidant capacity of EA were clarified using different analytical methodologies such as total antioxidant activity determination by ferric thiocyanate, hydrogen peroxide scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,2‧-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and superoxide anion radical scavenging, ferrous ions (Fe2+) chelating activity and ferric ions (Fe3+) reducing ability. EA inhibited 71.2% lipid peroxidation of a linoleic acid emulsion at 45 μg/mL concentration. On the other hand, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), α-tocopherol and ascorbic acid displayed 69.8%, 66.8%, 64.5% and 59.7% inhibition on the peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, EA had an effective DPPH• scavenging, ABTSrad + scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ions (Fe3+) reducing power and ferrous ions (Fe2+) chelating activities. Also, those various antioxidant activities were compared to BHA, BHT, α-tocopherol and ascorbic acid as references antioxidant compounds. These results suggested that EA can be used in the pharmacological, food industry and medicine because of these properties.

  8. The antioxidant activity of selected wild orchids of Nepal

    Directory of Open Access Journals (Sweden)

    Mukesh Babu Chand

    2016-09-01

    Full Text Available Objective: To assess the antioxidant activity as well as the total polyphenolics and flavonoids content of thirteen extracts from nine wild orchids of Nepal. Methods: First, a preliminary phytochemical screening of thirteen extracts of nine orchids was carried out using established protocols. Then, the total polyphenolic and flavonoid contents of each extract were evaluated using the Folin-Ciocalteu's phenol reagent and aluminium chloride methods respectively. Finally, antioxidant activity was determined by 2, 2-diphenyl-1-picryl hydrazyl free radical scavenging assay and expressed in terms of half inhibition concentrations (IC50 values. Results: All the tested parameters showed significant variation at P = 0.05. The total flavonoids varied with the greatest amounts found in the leaves of Rhynchostylis retusa and the smallest amounts found in the roots of Gastrochilus acutifolius (G. acutifolius. The amount of total polyphenolics was highest in the stems of Vanda cristata and lowest in the leaves of G. acutifolius. Antioxidant activity was highest in the stems of Vanda cristata and lowest in the leaves of G. acutifolius. Conclusions: Some of the orchid extracts studied exhibited levels of antioxidant activity which were comparable to or even higher than those found for extracts of medicinal plants and orchids studied previously. It is suggested that drugs and natural food additives can be extracted from those wild orchids with high levels of antioxidant activity.

  9. Phenolic Extracts from Acacia mangium Bark and Their Antioxidant Activities

    Directory of Open Access Journals (Sweden)

    Liangliang Zhang

    2010-05-01

    Full Text Available Phenolic compounds are present at very high concentrations in the bark of Acacia mangium. These compounds are known to have strong antioxidant activity and thus different beneficial effects on human health. Phenolic compounds in bark of A. mangium were extracted and their antioxidant activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical-scavenging and ferric-reducing antioxidant power (FRAP assays. A central composite design has been employed to optimize the experimental conditions for a high total phenolic content and antioxidant activity. The desirability function approach has been employed to simultaneously optimize the three responses: total phenols, antiradical activity and FRAP. An extraction time of 90 min, liquid-solid ratio of 5, and temperature of 50 °C was predicted for the optimum experimental conditions using the desirability function. A significant linear relationship between antioxidant potency, antiradical activity and the content of phenolic compounds of bark extracts was observed. The structures of condensed tannins isolated from A. mangium were characterized by MALDI-TOF MS analyses. Condensed tannin oligomers from A. mangium were shown to be heterogeneous mixtures consisting of procyanidin and prodelphinidin structural units with polymerization degrees up to 9.

  10. Phenolic extracts from Acacia mangium bark and their antioxidant activities.

    Science.gov (United States)

    Zhang, Liangliang; Chen, Jiahong; Wang, Yongmei; Wu, Dongmei; Xu, Man

    2010-05-14

    Phenolic compounds are present at very high concentrations in the bark of Acacia mangium. These compounds are known to have strong antioxidant activity and thus different beneficial effects on human health. Phenolic compounds in bark of A. mangium were extracted and their antioxidant activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging and ferric-reducing antioxidant power (FRAP) assays. A central composite design has been employed to optimize the experimental conditions for a high total phenolic content and antioxidant activity. The desirability function approach has been employed to simultaneously optimize the three responses: total phenols, antiradical activity and FRAP. An extraction time of 90 min, liquid-solid ratio of 5, and temperature of 50 degrees C was predicted for the optimum experimental conditions using the desirability function. A significant linear relationship between antioxidant potency, antiradical activity and the content of phenolic compounds of bark extracts was observed. The structures of condensed tannins isolated from A. mangium were characterized by MALDI-TOF MS analyses. Condensed tannin oligomers from A. mangium were shown to be heterogeneous mixtures consisting of procyanidin and prodelphinidin structural units with polymerization degrees up to 9.

  11. Relationship between Antioxidant and Anticancer Activity of Trihydroxyflavones

    Directory of Open Access Journals (Sweden)

    Ignas Grigalius

    2017-12-01

    Full Text Available Plant polyphenols have been highlighted not only as chemopreventive, but also as potential anticancer substances. Flavones are a subclass of natural flavonoids reported to have an antioxidant and anticancer activity. The aim of our study was to evaluate antioxidant and anticancer activity of seventeen trihydroxyflavone derivatives, including apigenin (API and baicalein (BCL. Also, we wanted to find out if there is a correlation between those two effects. Cell growth inhibition testing was carried out using MTT assay in three different human cancer cell lines: lung (A549, breast (MCF-7 and brain epithelial (U87. Antioxidant activity was determined by the DPPH radical scavenging method. Thirteen trihydroxyflavones possessed anticancer activity against at least one tested cancer cell line. They were more active against the MCF-7 cell line, and the lowest activity was determined against the U87 cell line. The majority of compounds inhibited cancer cell growth at EC50 values between 10–50 µM. The most active compound was 3’,4’,5-trihydroxyflavone 7, especially against A549 and MCF-7 cell lines. The correlation between anti-proliferative and antioxidant activity was only moderate, and it was determined for A549 and U87 cancer cell lines. The most important fragment for those two effects is the ortho-dihydroxy group in ring B. Conclusions. Trihydroxyflavones demonstrated anticancer activity. Further and more detailed studies should to be carried out to estimate the structure–activity relationship of these compounds.

  12. In Vivo Antioxidant Activity of Deacetylasperulosidic Acid in Noni

    Directory of Open Access Journals (Sweden)

    De-Lu Ma

    2013-01-01

    Full Text Available Deacetylasperulosidic acid (DAA is a major phytochemical constituent of Morinda citrifolia (noni fruit. Noni juice has demonstrated antioxidant activity in vivo and in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group, 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activity in vivo but additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

  13. Antioxidant and Anti-Inflammatory Activities of Barettin

    Directory of Open Access Journals (Sweden)

    Trond Ø. Jørgensen

    2013-07-01

    Full Text Available In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activity. Barettin was also able to inhibit the secretion of the inflammatory cytokines IL-1β and TNFα from LPS-stimulated THP-1 cells. This combination of anti-inflammatory and antioxidant activities could indicate that barettin has an atheroprotective effect and may therefore be an interesting product to prevent development of atherosclerosis.

  14. Antioxidant and anti-inflammatory activities of barettin.

    Science.gov (United States)

    Lind, Karianne F; Hansen, Espen; Østerud, Bjarne; Eilertsen, Karl-Erik; Bayer, Annette; Engqvist, Magnus; Leszczak, Kinga; Jørgensen, Trond Ø; Andersen, Jeanette H

    2013-07-22

    In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activity. Barettin was also able to inhibit the secretion of the inflammatory cytokines IL-1β and TNFα from LPS-stimulated THP-1 cells. This combination of anti-inflammatory and antioxidant activities could indicate that barettin has an atheroprotective effect and may therefore be an interesting product to prevent development of atherosclerosis.

  15. Studies on the antioxidant activity of Echinacea root extract.

    Science.gov (United States)

    Hu, C; Kitts, D D

    2000-05-01

    Methanol extracts of freeze-dried Echinacea (E. angustifolia, E. pallida, and E. purpurea) roots were examined for free radical scavenging capacities and antioxidant activities. Root extracts of E. angustifolia, E. pallida, and E. purpurea were capable of scavenging hydroxyl radical. Similar scavenging activities for each variety were found for both 1,1-diphenyl-2-picrylhydrazyl radical and ABTS radical. Meanwhile, antioxidant activities of all three varieties of Echinacea were found to delay the formation of conjugated diene hydroperoxide induced by the thermal decomposition of 2, 2'-azobis(2-amidinopropane) dihydrochloride and extend the lag phase of peroxidation of soybean liposomes. Echinacea root extracts suppressed the oxidation of human low-density lipoprotein, as evaluated by reduced agarose electrophoretic mobility following oxidative modification by Cu(2+). The mechanisms of antioxidant activity of extracts derived from Echinacea roots included free radical scavenging and transition metal chelating.

  16. In vitro synergistic antioxidant activity and identification of antioxidant components from Astragalus membranaceus and Paeonia lactiflora.

    Directory of Open Access Journals (Sweden)

    Xiaoyan Xu

    Full Text Available Many traditionally used herbs demonstrate significantly better pharmacological effects when used in combination than when used alone. However, the mechanism underlying this synergism is still poorly understood. This study aimed to investigate the synergistic antioxidant activity of Astragalus membranaceus (AME and Paeonia Lactiflora (PL, and identify the potential antioxidant components by 1,1-diphenyl-2-picrylhydrazine (DPPH radical spiking test followed by a high performance liquid chromatography separation combined with diode array detection and tandem mass spectrometry analysis (DPPH-HPLC-DAD-MS/MS. Eight AME-PL combined extracts (E1-E8 were prepared based on bioactivity-guided fractionation. Among them, E1 exhibited the strongest synergistic effect in scavenging DPPH radicals and reducing ferric ions (P<0.05. Moreover, E1 presented strong cytoprotection against H2O2-induced oxidative damage in MRC-5 cells by suppressing the decrease of the superoxide dismutase (SOD, glutathione peroxidase (GSH-Px and catalase (CAT activities. A strong correlation between the increment of total phenolic/flavonoid and synergistic antioxidant activity, especially between the increment of total flavonoid and the increase in ferric reducing power was observed. Finally, seven antioxidant substances were identified in E1 as oxypaeoniflora, catechin, calycosin-7-O-β-D-glucopyranoside, fomononetin-7-O-β-D-glucopyranoside, 9,10-dimethoxy-pterocarpan-3-O-β-D-glucopyranoside, quercetin and 2'-dihydroxy-3',4'-dimethyl-isoflavan-7-O-β-D-glucopyranoside.

  17. pH dependent antioxidant activity of lettuce (L. sativa) and synergism with added phenolic antioxidants.

    Science.gov (United States)

    Altunkaya, Arzu; Gökmen, Vural; Skibsted, Leif H

    2016-01-01

    Influence of pH on the antioxidant activities of combinations of lettuce extract (LE) with quercetin (QC), green tea extract (GTE) or grape seed extract (GSE) was investigated for both reduction of Fremy's salt in aqueous solution using direct electron spin resonance (ESR) spectroscopy and in L-α-phosphatidylcholine liposome peroxidation assay measured following formation of conjugated dienes. All examined phenolic antioxidants showed increasing radical scavenging effect with increasing pH values by using both methods. QC, GTE and GSE acted synergistically in combination with LE against oxidation of peroxidating liposomes and with QC showing the largest effect. The pH dependent increase of the antioxidant activity of the phenols is due to an increase of their electron-donating ability upon deprotonation and to their stabilization in alkaline solutions leading to polymerization reaction. Such polymerization reactions of polyphenolic antioxidants can form new oxidizable -OH moieties in their polymeric products resulting in a higher radical scavenging activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Antiproliferation effects and antioxidant activity of two new Lactobacillus strains

    Directory of Open Access Journals (Sweden)

    Parisa Shokryazdan

    2017-11-01

    Full Text Available Abstract The microorganisms most commonly used as probiotics are lactic acid bacteria, especially those of the genus Lactobacillus. In the present study, two Lactobacillus strains, L. pentosus ITA23 and L. acidipiscis ITA44, previously isolated from mulberry silage, were characterized for their antiproliferative and antioxidant activities. The antiproliferative effects of the strains were investigated using the MTT assay with breast cancer (MDA-MB-231, liver cancer (HepG2 and normal liver (Chang cell lines. The strains were tested for their antioxidant activity using the FRAP and ABTS methods. The results showed that the two Lactobacillus strains had good antiproliferative effects against both cancer cell lines tested, while their effects on the normal cells were weak. Based on the results of the antioxidant tests, the intact cells and cell-free extracts of the two Lactobacillus strains showed more than 135 and less than 50 µg trolox/ml of antioxidant activity, respectively. Lactobacillus pentosus ITA23 and L. acidipiscis ITA44 can be considered as potential probiotic candidates for humans because of their antioxidant activity and antiproliferation effects against cancer cells.

  19. Antioxidant Activities of Three Rubus Species Growing in Ethiopia ...

    African Journals Online (AJOL)

    80% methanol extract, and acetone and methanol soxhlet fractions of the three plants tested showed antioxidant activity in a concentration-dependent manner. The 80% methanolic extract of R. steudneri showed the strongest activity in the DPPH radical scavenging assay (IC50 = 6.5 mg/ml). In the deoxyribose degradation ...

  20. Antioxidant activity of Piper caninum and Cyclooxygenase-2 ...

    African Journals Online (AJOL)

    Background: This study investigated on antioxidant activity of Piper caninum and cyclooxygenase-2 inhibition by methoxylated flavones. Materials and methods: The present study was carried out to quantify the total phenolic content and free radical scavenging activities of the crude extracts by Folin-Ciocalteu and 1 ...

  1. In vitro Antifungal, Antioxidant and Cytotoxic Activities of a Partially ...

    African Journals Online (AJOL)

    higher antifungal activity on Candida albicans than on Aspergillus fumigatus. AMP III fraction showed greater in vitro antioxidant activity than the aqueous extract. SDS-PAGE analyses revealed the presence of two protein bands with molecular weight approximately of 16 and 67 KDa in AMP III. Protein concentration was 240 ...

  2. Antioxidant Activities of Methanol Extract and Solvent Fractions of ...

    African Journals Online (AJOL)

    2015-02-09

    Feb 9, 2015 ... and weak blood anticoagulant activity [12] detected in A. erecta extracts, no other bioactivities have been reported for the species. Extracts and compounds isolated from other. Avrainvillea species have been found to exhibit antioxidant activity [10]. It is therefore likely that. A. erecta may also contain potent ...

  3. Comparative profiling of biomarker psoralen in antioxidant active ...

    African Journals Online (AJOL)

    Comparative profiling of biomarker psoralen in antioxidant active extracts of different species of genus Ficus by validated HPTLC method. ... an important tool to assure the therapeutic dose of active ingredients in herbal formulations as well as for standardization and quality control of bulk drugs and in-process formulations.

  4. Antioxidant activity of acetone and ethanolic leaves extracts of ...

    African Journals Online (AJOL)

    The measured antioxidant properties included free radical scavenging activities against 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-3- ethylbenzothizoline 6-sulfonic acid (ABTS), ferric reducing power activities, hydrogen peroxide, nitric oxide scavenging properties and lipid peroxidation. The results show that the ...

  5. Characterization and Antioxidant Activity of the Complex of ...

    African Journals Online (AJOL)

    infrared spectrometry (IR), differential scanning calorimetry (DSC) and x-ray diffractometry (XRD). The antioxidant activity was examined by DPPH and ABTS radical-scavenging activities. Results: Phloridzin in the complex was molecularly dispersed in HP-β-CD matrix. The complex was an effective scavenger of DPPH and ...

  6. Antimicrobial and antioxidant activities of Momordica charantia from ...

    African Journals Online (AJOL)

    In the present study, unripe/ripe seed and fruit ethanol extracts of M. charantia from Turkey were screened for their potential antimicrobial and antioxidant activities. The antimicrobial activities of the extract were determined against four gram positive bacteria, seven gram negative bacteria, and one yeast with disc diffusion ...

  7. Antioxidant activity in some selected Indian medicinal plants | Kumar ...

    African Journals Online (AJOL)

    The study was carried out to determine the antioxidant activity of selected medicinal plants namely Albizia amara, Achyranthes aspera, Cassia fistula, Cassia auriculata and Datura stramonium by inhibition of lipid peroxidation technique. The highest inhibition of lipid peroxidation activity was observed in A. amara (96%) ...

  8. Comparative study of in vitro antioxidant activity of foliar endophytic ...

    African Journals Online (AJOL)

    Endophytic fungi that reside in plant tissues are a potential source of secondary metabolites with biological activities. In our study, we investigated the detection of the antioxidant activity of the crude fungal extract of the genera Cladosporium, Alternaria, Aspergillus and Penicillium: endophytic fungi isolated from the leaves of ...

  9. Cytotoxic, anti-inflammatory and antioxidant activities of four different ...

    African Journals Online (AJOL)

    Purpose: To evaluate the cytotoxic, anti-inflammatory and antioxidant activities of four different solvent extracts obtained from the aerial parts of Galega officinalis L. Methods: The hexane, DCM, methanol and water extracts of G. officinalis were successively obtained by soxhlet extraction method. The cytotoxic activity of the ...

  10. Evaluation of the antioxidant and antityrosinase activities of three ...

    African Journals Online (AJOL)

    The antioxidant activities were performed on - carotene-linoleic acid, reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferrous ions chelating abilities and xanthine oxidase inhibitory activity. In addition to this, phenolic compounds were also detected. The acetonic and methanolic extracts of P. nebrodensis showed the ...

  11. Evaluation of Antioxidant and Antifungal Activities of Polyphenol-rich ...

    African Journals Online (AJOL)

    Purpose: To evaluate the antioxidant and antifungal activities of polyphenol-rich extracts of the dried fruit pulp of Garcinia pedunculata (GP) and Garcinia morella (GM) to determine their traditional claims of therapeutic activity against certain diseases. Methods: Analysis of total phenolic (TP) and flavonoid (TF) contents of the ...

  12. Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae

    Directory of Open Access Journals (Sweden)

    F. Xavier Malcata

    2013-04-01

    Full Text Available A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae and 23 species of (eukaryotic microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05 total antioxidant capacity (149 ± 47 AAU of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II-ascorbic acid; and against decay in bacteriophage infection capacity induced by H2O2. Finally, performance of an Ames test revealed no mutagenic effects of the said extract.

  13. Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods.

    Directory of Open Access Journals (Sweden)

    Jiali Xing

    Full Text Available Antioxidant activity of lactic acid bacteria is associated with multiple health-protective effects. Traditional indexes of chemical antioxidant activities poorly reflect the antioxidant effects of these bacteria in vivo. Cellular antioxidant activity (CAA assay was used in this study to determine the antioxidant activity of cell-free supernatants (CFSs of 10 Lactobacillus strains. The performance of the CAA assay was compared with that of four chemical antioxidant activity assays, namely, DPPH radical scavenging, hydroxyl radical scavenging (HRS, reducing power (RP, and inhibition of linoleic acid peroxidation (ILAP. Results of the CAA assay were associated with those of DPPH and ILAP assays, but not with those of RP and HRS assays. The inter- and intra-specific antioxidant activities of CFS were characterized by chemical and CAA assays. L. rhamnosus CCFM 1107 displayed a high antioxidative effect similar to positive control L. rhamnosus GG ATCC 53103 in all of the assays. The CAA assay is a potential method for the detection of antioxidant activities of lactobacilli CFSs.

  14. Antioxidant Activity of Phenolic Compounds from Fava Bean Sprouts.

    Science.gov (United States)

    Okumura, Koharu; Hosoya, Takahiro; Kawarazaki, Kai; Izawa, Norihiko; Kumazawa, Shigenori

    2016-06-01

    Fava beans are eaten all over the world and recently, marketing for their sprouts began in Japan. Fava bean sprouts contain more polyphenols and l-3,4-dihydroxyphenylalanine (l-DOPA) than the bean itself. Our antioxidant screening program has shown that fava bean sprouts also possess a higher antioxidant activity than other commercially available sprouts and mature beans. However, the individual constituents of fava bean sprouts are not entirely known. In the present study, we investigated the phenolic compounds of fava bean sprouts and their antioxidant activity. Air-dried fava bean sprouts were treated with 80% methanol and the extract was partitioned in water with chloroform and ethyl acetate. HPLC analysis had shown that the ethyl acetate-soluble parts contained phenolic compounds, separated by preparative HPLC to yield 5 compounds (1-5). Structural analysis using NMR and MS revealed that the compounds isolated were kaempferol glycosides. All isolated compounds had an α-rhamnose at the C-7 position with different sugars attached at the C-3 position. Compounds 1-5 had β-galactose, β-glucose, α-rhamnose, 6-acetyl-β-galactose and 6-acetyl-β-glucose, respectively, at the C-3 position. The amount of l-DOPA in fava bean sprouts was determined by the quantitative (1) H NMR technique. The l-DOPA content was 550.45 mg ± 11.34 /100 g of the raw sprouts. The antioxidant activities of compounds 2-5 and l-DOPA were evaluated using the 2,2-diphenyl-1-picrylhydrazyl scavenging assay. l-DOPA showed high antioxidant activity, but the isolated kaempferol glycosides showed weak activity. Therefore, it can be suggested that l-DOPA contributed to the antioxidant activity of fava bean sprouts. © 2016 Institute of Food Technologists®

  15. Antioxidant activity and potential photoprotective from amazon native flora extracts.

    Science.gov (United States)

    Martins, Francislene J; Caneschi, César A; Vieira, José L F; Barbosa, Wagner; Raposo, Nádia R B

    2016-08-01

    Plant species are sources of active compounds that can fight and/or prevent damage caused by reactive oxygen species, which enables the development of natural products that can help to prevent premature aging caused by exposure to solar radiation. This study assessed the antioxidant and photoprotective activities of six dried extracts of plants from the Brazilian Amazon biome. Plant extracts were prepared in 70% (v/v) ethanol by dynamic maceration for 72h in the dark, and then filtered, concentrated and lyophilized. The extracts were subjected to a phytochemical screening. The antioxidant activity was measured using a 2,2-diphenyl-1-picrylhydrazyl assay and the photoprotection assay was performed using the diffuse transmittance technique. The data obtained from the antioxidant activity assay was evaluated by Student's t-test for independent samples, with the aid of Statistical Package for Social Sciences v.14.0 for Windows software. The flavonoids represent a special metabolites class present in all analyzed extracts. The antioxidant activity (μgmL(-1)) decreased in the following order: Aniba canelilla (1.80±0.16), Brosimum acutifolium (2.84±0.38), Dalbergia monetaria (5.46±0.17) or Caesalpinia pyramidalis (6.45±1.18), Arrabidaea chica (15.35±0.86), and Aspidosperma nitidum (99.14±2.3). Only D. monetaria showed a considerable sun protection factor allowing for labeling (6.0±0.3). The D. monetaria extract was considered the most promising sample because it had optimal antioxidant and photoprotective activities against solar radiation, considering the limit established by regulatory agencies. These extracts with antioxidant potential can be used in photoprotective formulations, providing synergistic photoprotective effect or elevating the adeed value of the product. Additionally, these formulations are attractive to a population who searchs for products made with natural ingredients. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. Evaluation of the Antioxidant Activity of the Marine Pyrroloiminoquinone Makaluvamines.

    Science.gov (United States)

    Alonso, Eva; Alvariño, Rebeca; Leirós, Marta; Tabudravu, Jioji N; Feussner, Klaus; Dam, Miriam A; Rateb, Mostafa E; Jaspars, Marcel; Botana, Luis M

    2016-10-27

    Makaluvamines are pyrroloiminoquinones isolated from Zyzzya sponges. Until now, they have been described as topoisomerase II inhibitors with cytotoxic effects in diverse tumor cell lines. In the present work, seven makaluvamines were tested in several antioxidant assays in primary cortical neurons and neuroblastoma cells. Among the alkaloids studied, makaluvamine J was the most active in all the assays. This compound was able to reduce the mitochondrial damage elicited by the well-known stressor H₂O₂. The antioxidant properties of makaluvamine J are related to an improvement of the endogenous antioxidant defenses of glutathione and catalase. SHSY5Y assays proved that this compound acts as a Nrf2 activator leading to an improvement of antioxidant defenses. A low concentration of 10 nM is able to reduce the reactive oxygen species release and maintain a correct mitochondrial function. Based on these results, non-substituted nitrogen in the pyrrole plus the presence of a p -hydroxystyryl without a double bond seems to be the most active structure with a complete antioxidant effect in neuronal cells.

  17. Synergistic antioxidant activity of green tea with some herbs

    Directory of Open Access Journals (Sweden)

    Dheeraj P Jain

    2011-01-01

    Full Text Available Cardiovascular diseases, cancer, arthritis, etc. are caused by free radicals that are byproducts of metabolic pathways. Selected plants namely Vitis vinifera, Phyllanthus emblica L., Punica granatum, Cinnamomum cassia, Ginkgo biloba L., and Camellia sinensis Linn. are reported to produce antioxidant property. This study is undertaken to support the hypothesis that formulation of a polyherbal combination of these plants shows a synergistic effect with green tea. The extracts of each drug were characterized by phytochemical studies and tests for phenolics and flavonoids. In vitro antioxidant activity for individual drug and its combination was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH, superoxide, and nitric oxide free radical scavenging methods. Our results suggest that a combination of all these herbs with green tea can synergistically enhance antioxidant activity and thus lower doses of each herb with green tea may be used. Antioxidant potential of polyherbal combination was also comparable to that of standard ascorbic acid. Studies showed that selected individual plants contained abundant quantity of phenolics and flavonoids and their polyherbal combination with green tea was found to produce best antioxidant activity among all individual extracts. This will help in avoiding undesirable side effects due to higher doses of single herb.

  18. Elimination of seaweed odour and its effect on antioxidant activity

    Science.gov (United States)

    Keyimu, Xiren Guli; Abdullah, Aminah

    2014-09-01

    The objectives of this study were to determine the most effective method to remove odour from Sargassum muticum seaweeds and studied their antioxidant properties. Ten grams of wet seaweeds (10 grams dried seaweeds soaked in 100 ml water for 2 hours) were soaked in 100 mL of 1%, 3% and 5% of gum Arabic, rice flour, lemon juice, respectively, and 1% of vinegar. There effect of each treatment on antioxidant level were determined by using the total phenolic content (TPC), free radical scavenging ability expressed as a DPPH value, and oxygen radical absorbance capacity (ORAC) and compared to control seaweeds sample (soaked in water only). For sensory attribute, seven trained panellists were asked to evaluate the fishy odour of 11 treated seaweed samples. The fishy odour characteristics and antioxidant activity of treated seaweeds were compared against the control sample (soaked seaweeds), and subjected to statistical analysis. Results showed that 3% and 5% lemon juice and 5% rice flour were able to eliminate the fishy odour of seaweed. However, the antioxidant activity was significantly higher (Plemon juice compared to other treatments. Therefore, 5% of lemon juice-treated seaweeds contained the least fishy odour and retained the highest antioxidant activity.

  19. Chemical Constituents and Antioxidant Activity of Teucrium barbeyanum Aschers

    Directory of Open Access Journals (Sweden)

    Mohamed Ali A. Alwahsh

    2015-01-01

    Full Text Available The different extracts of the aerial parts of Teucrium barbeyanum Aschers. were investigated for the chemical constituents and antioxidant activities. The chemical investigation of the plant led to the isolation of eleven known compounds through column chromatography in which nine were flavonoids and the other two were simple phenolic compounds. The compounds were characterized using NMR techniques ( 1H, 13C, DEPT-135 and 90, COSY, HMQC, HMBC and NOESY, UV spectroscopy and EI/ESI spectrometry. The isolated compounds were identified as 5-hydroxy-3,6,7,4′-tetramethoxyflavone (1, salvigenin (2, 5-hydroxy-6,7,3′,4′-tetramethoxyflavone (3, chrysosplenetin (4, cirsilineol (5, cirsimaritin (6, cirsiliol (7, apigenin (8 and luteolin (9, in addition to methyl caffeate (10 and 4-hydroxybenzoic acid (11. The antioxidant activit of the extracts was evaluated using 2,2-Diphenyl,1-picrylhydrazyl (DPPH, 2,2′-Azino-bis(3-ethylbenzothiazoline 6 sulfonic acid diammonium salt (ABTS and ferric reducing antioxidant power (FRAP methods. Ethyl acetate and butanol extracts showed comparable antioxidant activity to known antioxidants; trolox and ascorbic acid and the highest total phenolic and flavonoid contents. The active components were extracted efficiently in 70% aqueous methanol after defatting procedure. This is the first time the aforementioned compounds are isolated from this plant, and there has been no previous report on the biological studies on this species.

  20. Evaluation of the Antioxidant Activity of the Marine Pyrroloiminoquinone Makaluvamines

    Directory of Open Access Journals (Sweden)

    Eva Alonso

    2016-10-01

    Full Text Available Makaluvamines are pyrroloiminoquinones isolated from Zyzzya sponges. Until now, they have been described as topoisomerase II inhibitors with cytotoxic effects in diverse tumor cell lines. In the present work, seven makaluvamines were tested in several antioxidant assays in primary cortical neurons and neuroblastoma cells. Among the alkaloids studied, makaluvamine J was the most active in all the assays. This compound was able to reduce the mitochondrial damage elicited by the well-known stressor H2O2. The antioxidant properties of makaluvamine J are related to an improvement of the endogenous antioxidant defenses of glutathione and catalase. SHSY5Y assays proved that this compound acts as a Nrf2 activator leading to an improvement of antioxidant defenses. A low concentration of 10 nM is able to reduce the reactive oxygen species release and maintain a correct mitochondrial function. Based on these results, non-substituted nitrogen in the pyrrole plus the presence of a p-hydroxystyryl without a double bond seems to be the most active structure with a complete antioxidant effect in neuronal cells.

  1. Liquid Fermentation of Ganoderma applanatum and Antioxidant Activity of Exopolysaccharides.

    Science.gov (United States)

    Zhong-Hua, Liu; Xiao-Ge, Hou; Jin-Hui, Zhao; Le, H E

    2015-01-01

    The medium composition and fermentation conditions of Ganoderma applanatum(GA) strain were optimized by the liquid shake flask fermentation, and the antioxidant activity of exopolysaccharides was investigated. The results showed that the optimal conditions of the liquid fermentation of GA strain were as follows: Carbon source was corn powder, nitrogen source was soy powder, the initial pH was 6.0, the inoculum size was 8%, the fermentation temperature was 32(o)C, the fermentation time was 7 d. The exopolysaccharides of GA strain could scavenge hydroxyl radicals(HR) and superoxide anion radicals(SAR), and the concentration of exopolysaccharides was positively related to the antioxidant activity.

  2. ANTIOXIDANT, ANTI-INFLAMMATORY ACTIVITY OF GEORGIAN LEGUMINOUS CROPS CULTURES.

    Science.gov (United States)

    Chkhikvishvili, I; Mamniashvili, T; Gogia, N; Enukidze, M; Machavariani, M; Sanikidze, T

    2017-11-01

    The aim of the study was to identify the common in Georgia leguminous crops culture with pronounced antioxidant, anti-inflammatory activity. The primary evaluation of the antiinflammatory effects of beans was performed on the experimental models of MDCK and Jurkat cells model systems. Extracts of various varieties of legumes (Beans "Kidney", Meadow beans, Beans Shulavera, Batumian beans, Beans "Udelebi", green peas, peas Shulavera, lentils Lens Culinaris, Soy) were added to the intact or incubated under oxidative stress conditions Jurkat and MDCK cells. Cells' vitality was determined by MTT test. On the basis of analysis of the obtained results, we concluded that: - Meadow beans extract (low doses) revealed cytoprotective effect on the intact and incubated under oxidative stress conditions immune (Jurkat) and epithelial (MDCK) cells. High antioxidant, cytoprotective activity of this extract correlates with high polyphenols content in it. - The extract of Shulavera beans did affect the intact Jurkat and MDCK cells, but showed pronounced cytoprotective activity on these cells incubated under the oxidative stress conditions. High antioxidant, cytoprotective activity of this extract correlates with high content of polyphenols in it. - Low dose of lentils Lens Culinaris extracts revealed cytoprotective activity on the incubated under oxidative stress conditions MDCK cells, but was inactive in case of intact MDCK and incubated in different conditions immune Jurkat cells. The selective antioxidant activity of this extract is related with its other constituent components, but not polyphenols. - Despite high polyphenols content and high antioxidant activity in vivo, Batumian beans revealed moderate cytoprotective activity on intact and incubated under oxidative stress conditions Jurkat cells, suppressive activity on the intact MDCK cells and was inactive in relation to the incubated under oxidative stress conditions MDCK cells. Based on these findings, we can identify

  3. Antioxidant and cytotoxic activities of three species of tropical seaweeds.

    Science.gov (United States)

    Chia, Yin Yin; Kanthimathi, M S; Khoo, Kong Soo; Rajarajeswaran, Jayakumar; Cheng, Hwee Ming; Yap, Wai Sum

    2015-09-29

    Three species of seaweeds (Padina tetrastromatica, Caulerpa racemosa and Turbinaria ornata) are widely consumed by Asians as nutraceutical food due to their antioxidant properties. Studies have shown that these seaweeds exhibit bioactivities which include antimicrobial, antiviral, anti-hypertensive and anticoagulant activities. However, investigations into the mechanisms of action pertaining to the cytotoxic activity of the seaweeds are limited. The aim of this study was to determine the antioxidant and cytotoxic activities of whole extracts of P. tetrastromatica, C. racemosa and T. ornata, including the cellular events leading to the apoptotic cell death of the extract treated-MCF-7 cells. Bioassay guided fractionation was carried out and the compounds identified. Powdered samples were sequentially extracted for 24 h. Their antioxidant activities were assessed by the DPPH radical, superoxide, nitric oxide and hydroxyl radical scavenging assays. The cytotoxic activity of the extract-treated MCF-7cells was assessed using the MTT assay. The most potent fraction was subjected to bioassay guided fractionation with column chromatography. All the fractions were tested for cytotoxic activity, caspase activity and effect on DNA fragmentation. All three seaweeds showed potent radical scavenging activities in the various assays. The activity of the cellular antioxidant enzymes, superoxide dismutase, catalase and glutathione reductase, in MCF-7 cells, decreased in a time-dependent manner. The partially purified fractions exhibited higher cytotoxic activity, as assessed by the MTT assay, than the whole extracts in the breast adenocarcinoma cell line, MCF-7. LC-MS analysis revealed the presence of bioactive alkaloids such as camptothecin, lycodine and pesudopelletierine. Based on the results obtained, all three seaweeds are rich sources of enzymatic and non-enzymatic antioxidants which could contribute to their reported medicinal benefits.

  4. Antioxidant potential of water hyacinth (Eichornia crassipes): In vitro antioxidant activity and phenolic composition

    DEFF Research Database (Denmark)

    Surendraraj, A.; Farvin, Sabeena; Anandan, R.

    2013-01-01

    The aims of the present study were (a) to extract and quantify the main phenolic acids and tocopherols from the petiole, leaves, and flowers of Eichornia crassipes; (b) to evaluate the antioxidant capacity of the extracts in four in vitro systems (1,1-diphenyl-2-pycryl-hydrazyl [DPPH] radical...... and in the antioxidant activities of extracts from the various parts of E. crassipes. Out of the 11 phenolic acids analyzed, ethanolic extracts contained high amounts of gallic, protocatechuic, gentisic, and p-hydroxybenzoic acid, whereas, water extracts contained less amounts of a varied number of phenolic acids....... Ethanolic extracts of flower, which contained the highest total phenolic content, were found to have high DPPH radical scavenging activity and reducing power. However, ethanolic extracts of leaf exerted a high Fe2+ chelating activity and also inhibited lipid peroxidation process both in liposomes and fish...

  5. Hop pellets as an interesting source of antioxidant active compounds

    Directory of Open Access Journals (Sweden)

    Andrea Holubková

    2013-02-01

    Full Text Available Hop is a plant used by humankind for thousands of years. This plant is one of the main and indispensable raw materials for the beer production. It is used for various dishes preparation in the cuisine. Hop is also used to inhibit bacterial contamination. The hop extracts are used for its sedative, antiseptic and antioxidant properties in medicine, as a part of many phytopharmaceuticals. The present paper have focused on the extraction of polyphenolic compounds from 4 samples of hop pellets varieties of Aurora, Saaz, Lublin and Saphir, on the analyzing of bioactive substances (polyphenolics and flavonoids in prepared extracts and on the determination of antioxidant activity.  The highest content of polyphenolic substances was determined in the sample Lublin (153.06 mg gallic acid (GAE/g and Saaz (151.87 mg GAE/g. The amount of flavonoids in the samples  was descending order Saaz > Saphir > Aurora > Lublin. Hops, as plant, is known by high content of antioxidant active substances. Antioxidant activity was determined using three independent spectrofotometric methods, radical scavenging assays using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS and 1,1-diphenyl-2-picrylhydrazyl (DPPH radical and ferric reducing antioxidant power (FRAP. The sample Aurora showed the highest ability to scavenge of ABTS radical cation. Antioxidant activity continued to decline in a row Saphir> Lublin> Saaz. The same trend was also observed by using the FRAP assay. The most effective DPPH radical scavengering activity had the sample Saaz a Saphir (p>0.05.doi:10.5219/270 Normal 0 21 false false false SK X-NONE X-NONE

  6. Compounds from Sedum caeruleum with antioxidant, anticholinesterase, and antibacterial activities.

    Science.gov (United States)

    Bensouici, Chawki; Kabouche, Ahmed; Karioti, Anastasia; Öztürk, Mehmet; Duru, Mehmet Emin; Bilia, Anna Rita; Kabouche, Zahia

    2016-01-01

    This is the first study on the phytochemistry, antioxidant, anticholinesterase, and antibacterial activities of Sedum caeruleum L. (Crassulaceae). The objective of this study is to isolate the secondary metabolites and determine the antioxidant, anticholinesterase, and antibacterial activities of S. caeruleum. Six compounds (1-6) were isolated from the extracts of S. caeruleum and elucidated using UV, 1D-, 2D-NMR, and MS techniques. Antioxidant activity was investigated using DPPH(•), CUPRAC, and ferrous-ions chelating assays. Anticholinesterase activity was determined against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes using the Ellman method. Antibacterial activity was performed according to disc diffusion and minimum inhibitory concentration (MIC) methods. Isolated compounds were elucidated as ursolic acid (1), daucosterol (2), β-sitosterol-3-O-β-D-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6). The butanol extract exhibited highest antioxidant activity in all tests (IC50 value: 28.35 ± 1.22 µg/mL in DPPH assay, IC50 value: 40.83 ± 2.24 µg/L in metal chelating activity, and IC50 value: 23.52 ± 0.44 µg/L in CUPRAC), and the highest BChE inhibitory activity (IC50 value: 36.89 ± 0.15 µg/L). Moreover, the chloroform extract mildly inhibited (MIC value: 80 µg/mL) the growth of all the tested bacterial strains. Ursolic acid (1), daucosterol (2), β-sitosterol-3-O-β-D-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6) were isolated from Sedum caeruleum for the first time. In addition, a correlation was observed between antioxidant and anticholinesterase activities of bioactive ingredients of this plant.

  7. Essential oil composition and antioxidant activity of Pterocarya fraxinifolia.

    Science.gov (United States)

    Ebrahimzadeh, M A; Nabavi, S F; Nabavi, S M

    2009-07-01

    Current research into free radicals has confirmed that plants rich in antioxidants play an essential role in the prevention of many diseases. The potential antioxidant activities of Pterocarya fraxinifolia bark and leaves investigated employing six in vitro assay systems. IC50 for DPPH radical-scavenging activities were 3.89 +/- 0.09 for leaves and 41.57 +/- 1.30 microg mL(-1) for bark, respectively. The leaf extract exhibited a good reducing power at 2.5 and 80 microg mL(-1) that was comparable with Vit C (p > 0.05). The extracts also showed weak nitric oxide-scavenging activity and Fe2+ chelating ability. The peroxidation inhibition of extracts exhibited values from 92 to 93% at 72nd h, almost at the same pattern of Vitamin C activity (p > 0.05). Based on higher total phenol and flavonoid contents in leaves, higher antioxidant activities were observed in leaf extract. In addition, chemical composition of leaf essential oil was determined. The major compound was bisabolol oxide A (23.6%). Sesquiterpenes and monoterpenes are the major compounds in leaves essential oil. Presence of these compounds may be a reason for the good antioxidant activity of leaf extract.

  8. Yucca schidigera bark: phenolic constituents and antioxidant activity.

    Science.gov (United States)

    Piacente, Sonia; Montoro, Paola; Oleszek, Wieslaw; Pizza, Cosimo

    2004-05-01

    Two new phenolic constituents with unusual spirostructures, named yuccaols D (1) and E (2), were isolated from the MeOH extract of Yucca schidigera bark. Their structures were established by spectroscopic (ESIMS and NMR) analysis. The new yuccaols D and E, along with resveratrol (3), trans-3,3',5,5'-tetrahydroxy-4'-methoxystilbene (4), yuccaols A-C (5-7), yuccaone A (8), larixinol (9), the MeOH extract of Yucca schidigera bark, and the phenolic portion of this extract, were assayed for antioxidant activity by measuring the free radical scavenging effects using two different assays, namely, the Trolox Equivalent Antioxidant Capacity (TEAC) assay and the coupled oxidation of beta-carotene and linoleic acid (autoxidation assay). The significant activities exhibited by the phenolic fraction and its constituents in both tests show the potential use of Y. schidigera as a source of antioxidant principles.

  9. Chilean prosopis mesocarp flour: phenolic profiling and antioxidant activity.

    Science.gov (United States)

    Schmeda-Hirschmann, Guillermo; Quispe, Cristina; Soriano, Maria Del Pilar C; Theoduloz, Cristina; Jiménez-Aspée, Felipe; Pérez, Maria Jorgelina; Cuello, Ana Soledad; Isla, Maria Inés

    2015-04-17

    In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic content ranging between 0.82-2.57 g gallic acid equivalents/100 g fresh flour weight. The highest antioxidant activity, measured by the DPPH assay, was observed for samples from the Huasco valley. HPLC-MS/MS analysis allowed the tentative identification of eight anthocyanins and 13 phenolic compounds including flavonol glycosides, C-glycosyl flavones and ellagic acid derivatives. The antioxidant activity and the phenolic composition in the flour suggest that this ancient South American resource may have potential as a functional food.

  10. Chilean Prosopis Mesocarp Flour: Phenolic Profiling and Antioxidant Activity

    Directory of Open Access Journals (Sweden)

    Guillermo Schmeda-Hirschmann

    2015-04-01

    Full Text Available In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic content ranging between 0.82–2.57 g gallic acid equivalents/100 g fresh flour weight. The highest antioxidant activity, measured by the DPPH assay, was observed for samples from the Huasco valley. HPLC-MS/MS analysis allowed the tentative identification of eight anthocyanins and 13 phenolic compounds including flavonol glycosides, C-glycosyl flavones and ellagic acid derivatives. The antioxidant activity and the phenolic composition in the flour suggest that this ancient South American resource may have potential as a functional food.

  11. Proliferative and antioxidant activity of Symphytum officinale root extract.

    Science.gov (United States)

    Sowa, Ireneusz; Paduch, Roman; Strzemski, Maciej; Zielińska, Sylwia; Rydzik-Strzemska, Ewelina; Sawicki, Jan; Kocjan, Ryszard; Polkowski, Janusz; Matkowski, Adam; Latalski, Michał; Wójciak-Kosior, Magdalena

    2018-03-01

    The root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce the inflammation and in the treatment of broken bones. The objective of our investigation was to analyse the extract from S. officinale in term of its antioxidant activity and the effect on cell viability and proliferation of human skin fibroblast (HSF). Moreover, the quantification of main phenolics and allantoin was conducted using HPLC-DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic and p-coumaric acid. DPPH, FRAP and TPC assay showed the high antioxidant activity of the extract. MTT test proved the stimulatory effect on cell metabolism and viability of HSF cells. Moreover, no changes in cytoskeleton structure and cells shape were observed. The obtained results indicate that non-toxic extract from S. officinale root has strong antioxidant potential and a beneficial effect on human skin fibroblasts.

  12. Comparison of Antioxidant Activities of Melanin Fractions from Chestnut Shell

    Directory of Open Access Journals (Sweden)

    Zeng-Yu Yao

    2016-04-01

    Full Text Available Chestnut shell melanin can be used as a colorant and antioxidant, and fractionated into three fractions (Fr. 1, Fr. 2, and Fr. 3 with different physicochemical properties. Antioxidant activities of the fractions were comparatively evaluated for the first time. The fractions exhibited different antioxidative potential in different evaluation systems. Fr. 1, which is only soluble in alkaline water, had the strongest peroxidation inhibition and superoxide anion scavenging activity; Fr. 2, which is soluble in alkaline water and hydrophilic organic solvents but insoluble in neutral and acidic water, had the greatest power to chelate ferrous ions; and Fr. 3, which is soluble both in hydrophilic organic solvents and in water at any pH conditions, had the greatest hydroxyl (·OH and 1,1-diphenyl-2-picryl-hydrazyl (DPPH· radicals scavenging abilities, reducing power, and phenolic content. The pigment fractions were superior to butylated hydroxytolune (BHT in ·OH and DPPH· scavenging and to ethylene diamine tetraacetic acid (EDTA in the Fe2+–chelation. They were inferior to BHT in peroxidation inhibition and O2·− scavenging and reducing power. However, BHT is a synthetic antioxidant and cannot play the colorant role. The melanin fractions might be used as effective biological antioxidant colorants.

  13. Antioxidant and anticholinesterase activities of eleven edible plants.

    Science.gov (United States)

    Boğa, Mehmet; Hacıbekiroğlu, Işıl; Kolak, Ufuk

    2011-03-01

    Consumers have become more interested in beneficial effects of vegetables, fruits, and tea to protect their health. The antioxidant potential and anticholinesterase activity of eleven edible plants were investigated. The dichloromethane, ethanol and water extracts prepared from celery [Apium graveolens L. (Umbelliferae)], Jerusalem artichoke [Helianthus tuberosus L. (Compositae)], spinach [Spinacia oleracea L. (Chenopodiaceae)], chard [Beta vulgaris L. var. cicla (Chenopodiaceae)], purslane [Portulaca oleracea L. (Portulacaceae)], ispit, or borage [Trachystemon orientale (L.) G. Don (Boraginaceae)], garden rocket [Eruca sativa Mill. (Brassicaceae)], red cabbage [Brassica oleracea L. var. capitata f. rubra DC. (Cruciferae)], lime flower [Tilia tomentosa Moench (Tiliaceae)], cinnamon [Cinnamomum cassia Presl. (Lauraceae)], and rosehip [Rosa canina L. (Rosaceae)], were tested to determine their antioxidant and anticholinesterase activities by using CUPRAC (cupric reducing antioxidant capacity) and Ellman methods, respectively, for the first time. As a result, the dichloromethane, ethanol and water extracts of cinnamon showed the best antioxidant effect among the extracts of the tested plants. The ethanol extract of cinnamon exhibited 63.02% inhibition against acetylcholinesterase and 85.11% inhibition against butyrylcholinesterase (BChE) at 200 µg/mL concentration while the dichloromethane extract of garden rocket possessed the highest inhibition (91.27%) against BChE among all the tested extracts. This study indicated that the ethanol extract of cinnamon may be a new potential resource of natural antioxidant and anticholinesterase compounds.

  14. Study of Convolvulus pluricaulis for antioxidant and anticonvulsant activity.

    Science.gov (United States)

    Verma, Sristi; Sinha, Reema; Kumar, Puspendra; Amin, Faizal; Jain, Jainendra; Tanwar, Shivani

    2012-03-01

    Convolvulus pluricaulis Choisy is a perennial wild herb commonly found on sandy & rocky areas under xerophytic conditions in northern India. It is a reputed drug of ayurveda and reported to posses antioxidant, brain tonic, nervine tonic, laxative and has been used in anxiety, neurosis, epilepsy, insomnia, burning sensation, oedema and urinary disorders. In the present study, methanolic extract of whole plant of Convolvulus pluricaulis Choisy was evaluated for antioxidant activity by using 1, 1-diphenyl-2-picryl- hydrazyl (DPPH) free radical scavenging model and anticonvulsant activity by using maximal electroshock seizure model. In antioxidant activity, ascorbic acid was used as standard agent while results of anticonvulsant studies were compared with phenytoin. Results of antioxidant activity have demonstrated significant free radical scavenging effect for methanolic extract of Convolvulus pluricaulis Choisy. IC50 value of methanolic extract was observed as 41.00μg/ml as compared to 2.03μg/ml of ascorbic acid. Methanolic extract of C. pluricaulis was evaluated for anticonvulsant activity at 250, 500 and 1000mg/kg. Experimental results have shown that at the dose of 500 and 1000mg/kg, C. pluricaulis didn't abolish the hind limb extension, but reduced the mean recovery time from convulsion.

  15. Anibacterial, antibiofilm and antioxidant activity of Potamogeton nodosus Poir. extracts

    Directory of Open Access Journals (Sweden)

    Đurđević Jelena

    2014-01-01

    Full Text Available Antibacterial, antibiofilm and antioxidant activity of acetone, ethanol, ethyl acetate and diethyl ether extracts of P. nodosus Poir. were tested. In vitro antibacterial activity was investigated by microdilution method. Minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC have been determined. The values were in the range from 5 to > 5 mg/ml. Antibiofilm activity was tested by crystal violet assay. The biofilm inhibitory concentration (BIC values were from 2.03 to > 10 mg/ml. The best results against biofilm formation had ethanol and ethyl acetate extracts. The total phenolic content was established by using Folin-Ciocalteu method. The highest total phenol content was found in the ethyl acetate extract (28.45 mg GA/g while the lowest content in the ethanol extract (22.74 mg GA/g. The concentrations of flavonoids were determined using aluminium chloride method. The concentration of total flavonoid content was 102.09 mg RU/g for the ethyl acetate extract, while the lowest content was noticed in diethyl ether extract (32.93 mg RU/g. The antioxidant activity was determined by the DPPH free radicals assay. Antioxidative efficiency was in the range from 641.77 to 8811.63 μg/ml. The tested extracts showed weak antioxidant activity (AAI < 0.5.

  16. Antioxidant potential of water hyacinth (Eichornia crassipes): In vitro antioxidant activity and phenolic composition

    DEFF Research Database (Denmark)

    Surendraraj, Alagarsamy; Farvin, Sabeena; Anandan, R.

    2011-01-01

    The aims of the present study were (a) to extract and quantify the main phenolic acids and tocopherols from the petiole, leaf and flowers of Eichornia crassipes, (b) to evaluate the antioxidant capacity of the extracts in four in vitro systems (DPPH radical scavenging ability, iron chelating...... activity, reducing power and prevention of oxidation in a liposome model system) and its effectiveness in retarding lipid peroxidation in fish oil by accelerated stability test. Significant differences were observed in total and individual phenolic content and antioxidant activities of extracts from...... the various parts of E. crassipes. Out of the 11 phenolic acids analysed, ethanolic extracts contained high amounts gallic, protocatechuic, gentisic and phydroxybenzoic acid, whereas, water extracts contained less amounts of varied number of phenolic acids. Ethanolic extracts of flower, which contained...

  17. Antimicrobial and antioxidant activity of lemon balm Kombucha

    OpenAIRE

    Velićanski Aleksandra S.; Cvetković Dragoljub D.; Markov Siniša L.; Tumbas Vesna T.; Savatović Slađana M.

    2007-01-01

    Kombucha is a beverage traditionally produced by metabolic activity of yeasts and acetic acid bacteria. The antimicrobial activity of lemon balm kombucha as well as of particular control samples was determined by agar-well diffusion method. Antioxidant activity on stable 1,1-diphenyl-2-picrylhydrazyl radicals of lemon balm kombucha and lemon balm tea was determined by electron spin resonance spectroscopy. Acetic acid, Kombucha samples and heat-denaturated kombucha showed significant antimicro...

  18. Essential Oil Composition, Antibacterial and Antioxidant Activities of ...

    African Journals Online (AJOL)

    picrylhydrazyl (DPPH) and deoxyribose degradation assays and was found to possess good radical scavenging activity with IC50 values of 11.2 and 3.74 μl/ml, respectively. Keywords: antibacterial, antioxidant, Mentha aquatica, essential oil, capillary gas ...

  19. Observation of antioxidant activity of leaves, callus and suspension ...

    African Journals Online (AJOL)

    GREGORY

    2011-12-16

    Dec 16, 2011 ... Antioxidant activity and phenolic compound was found in Justicia gendarussa via total phenolic content (TPC) and α,α-diphenyl-β-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture.

  20. Antibacterial and Antioxidant Activity of Fahrenheitia zeylanica (Thw ...

    African Journals Online (AJOL)

    Fahrenheitia zeylanica (Thw.) Airy is a lofty evergreen tree belonging to the family Euphorbiaceae. The present study was conducted to evaluate antibacterial and antioxidant activity of solvent extracts of leaves of F. zeylanica. The powdered leaf material was sequentially extracted using ethyl acetate, chloroform and ...

  1. Nanoemulsion of ethanolic extracts of propolis and its antioxidant activity

    Science.gov (United States)

    Mauludin, R.; Primaviri, D. S.; Fidrianny, I.

    2015-09-01

    Propolis contains several antioxidant compounds which can be used in topical application to protect skin against free radical and prevent skin cancer and skin aging. Ethanolic extracts of propolis (EEP) provided the greatest antioxidant activity but has very small solubility in water thus was prepared in nanoemulsion (NE). EEP contains steroid/triterpenoid, flavonoid, and saponin. EEP had the value of DPPH scavenging activity 61.14% and IC50 0.41629 ppm. The best NE formulation consisted of 26.25% Kolliphor RH40; 8.75% glycerin; 5% rice bran oil; and 3% EEP. NE was transparent, had particle size of 23.72 nm and polydispersity index of 0.338. Based on TEM morphology, NE was almost spherical and has particle size below 50 nm. NE propolis revealed to be physically stable after stability test within 63 days at 25°C and passed 6 cycles of Freeze and Thaw test without separated. NE propolis reduced around 58% of free radical DPPH similar to antioxidant activity of the original extracts. Antioxidant activity of NE propolis is relatively stable after stored for 6 weeks. NE propolis was proven to be safe by primary irritation test with the value of primary irritation index (OECD) was 0.

  2. Extraction and antioxidant activity of phenolic compounds from ...

    African Journals Online (AJOL)

    Purpose: To use response surface methodology to optimize the extraction of the phenolic compounds in wheat bran treated by steam explosion, and to determine the antioxidant activity of the extract obtained. Methods: By using response surface methodology, the effects of extraction time, methanol concentration ...

  3. Ultrasonic-Assisted Extraction and Antioxidant Activity of Flavonoids ...

    African Journals Online (AJOL)

    Purpose: To identify the main flavonoid in Adinandra nitida leaf, employ response surface methodology to optimize its ultrasonic-assisted extraction, and determine the antioxidant activity of the obtained extract. Methods: The main flavonoid in Adinandra nitida leaf was obtained by traditional solvent extraction and ...

  4. Extraction and antioxidant activities of two species Origanum plant ...

    African Journals Online (AJOL)

    The antioxidant of ethanolic extract of two species of Origanum and essential oil of plant Origanum vulgare were investigated and also the total phenolic and flavonoid content measured. The radical scavenging activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic and flavonoid ...

  5. Determination of antioxidant activity in methanolic and chloroformic ...

    African Journals Online (AJOL)

    Administrator

    2011-06-06

    Jun 6, 2011 ... The total antioxidant activity results indicated that, the inhibition percent of methanolic extract was significantly higher than the inhibition percent of chloroformic extract in the FTC and TBA methods. A higher IC50 value for free radical scavenging was found for methanolic extract when compared with ...

  6. Chemical compositions and antioxidant activity of Heracleum persicum essential oil

    Directory of Open Access Journals (Sweden)

    Maryam Gharachorloo

    2018-02-01

    Full Text Available ABSTRACT In this study essential oil of the aerial parts of Heracleum persicum a spice widely used in Iran was isolated by conventional hydrodistillation (HD and microwave-assisted hydrodistillation (MAHD techniques. The extraction yield was determined and the chemical compositions of essential oils were identified by the application of gas chromatography/mass spectrometry (GC/MS. The antioxidant activity was determined by two different methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical scavenging and oven test methods. Although the main compounds of essential oils by the both extraction methods were similar, the essential oil extracted by HD with lower extraction efficiency showed more diverse compounds. The evaluation of antioxidant activity of the essential oil measured by delay in sunflower oil oxidation indicated that the antioxidant activity was dependent on the concentration which increased when higher concentrations of the essential oils were applied. The results of DPPH radical assay also indicated that the percentage of inhibition increased with increasing of essential oil concentration and IC50 value for essential oil extracted by MAHD method was obtained 1.25 mg/mL. Therefore the Heracleum persicum essential oil might be recommended for use as a flavoring agent and a source of natural antioxidants in functional foods, formulation of the supplements and in medicinal due to numerous pharmacological activities.

  7. In vitro antioxidant and free radical scavenging activity of Leonurus ...

    African Journals Online (AJOL)

    The total phenolic compounds in the extracts were determined as gallic acid equivalents, and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Keywords: Antioxidant activity, Fe2+ chelating, 1,1-diphenyl-2-picryl hydrazyl (DPPH), phenol, Leonurus cardiaca, Grammosciadium ...

  8. rich extract on total polyphenols and antioxidant activity obtained by ...

    African Journals Online (AJOL)

    Z. Ghouila

    USTHB, Organic Functional Analysis Laboratory, 16111 Bab Ezzouar, Algiers, Algeria. 3. Department of General ... Keywords: Ahmeur Bouamer, extraction, grape seeds, total polyphenols, antioxidant activity. ... in food and pharmaceutical industry like BHA and BHT, which their undesirable effect on health was often ...

  9. Antioxidant activity study and total phenolic determination of leaf ...

    African Journals Online (AJOL)

    Methodology and results: This work was used to study the antioxidant activity of two extracts of Ximenia americana (Tallow wood) by the methods of ABTS•+ and DPPH using ascorbic acid as standard. It determined the rate of total phenolic in these extracts by the Folin-Ciocalteu (FC) method using Gallic acid as standard.

  10. In vitro antioxidant and antimicrobial activities of aerial parts of ...

    African Journals Online (AJOL)

    Folin-Ciocalteu and aluminum chloride procedures were used to quantify the total phenolic and flavonoid contents, respectively, while antioxidant properties were determined by two methods: phosphomolybdenum and DPPH assays. Antimicrobial activity was also evaluated by disc diffusion method. Results: The ethyl ...

  11. Antioxidant and Antimicrobial Activities of Flavonoid Glycosides from ...

    African Journals Online (AJOL)

    Dennettia tripetala a Nigerian medicinal plant widely employed in the management of oxidative stress related diseases and infections was investigated for its active constituents. A DPPH (1,1-diphenyl-2-picrylhydrazine) guided fractionation was used to target and isolate the antioxidant constituents of the ethyl acetate ...

  12. Evaluation of proximate and antioxidant activities of Ethiopian ...

    African Journals Online (AJOL)

    ... domesticated for their leaves, fruits or both; eaten as vegetables or used in traditional medicine. However, S. aethiopicum and S. macrocarpum are the most cultivated and most utilized in Nigeria. Nutritional composition, phytochemical screening and antioxidant activity of S. aethiopicum and S. macrocarpon were studied.

  13. Evaluation of phytochemical constituents and antioxidant activity of ...

    Indian Academy of Sciences (India)

    2013-10-01

    Oct 1, 2013 ... stress (Goyal et al. 2010). Polyphenols, naturally occurring in many fruits, account for the majority of antioxidant activity (Li et al. 2012). However, polyphenols can under- go various reactions in the course of ..... ethnomedicinal species in a subtropical forest of Meghalaya, northeast India. Asian Pac. J. Trop.

  14. Improvement of nutritional quality and antioxidant activities of yeast ...

    African Journals Online (AJOL)

    This study evaluated the potential use and improves the health beneficial properties of the soybean waste manufacturing products by solid-state fermentation of six GRAS different yeast strains, including extractable antioxidant activities and bioavailable nutritional compositions. In comparison with nonfermented okara ...

  15. In vitro antioxidant activity and phytochemical screening of methanol ...

    African Journals Online (AJOL)

    Both extracts showed no significant differences (p > 0.05) in their levels of thiobarbituric acid reactive substances (TBARS). The results suggest that D. edulis and F. capensis leaves possess ample antioxidant activity and phytochemical constituents which may play important roles in the pharmaceutical and food industries.

  16. Antioxidant activities of seed extracts from Dalbergia odorifera T. Chen

    African Journals Online (AJOL)

    The heartwood or root of Dalbergia odorifera T. Chen is an important traditional Chinese medicine. Antioxidant activities of seed extracts from D. odorifera T. Chen were first investigated in this study. Ethanolic extracts were suspended in distilled water and partitioned successively with petroleum ether, ethyl acetate, ...

  17. Antimicrobial and Antioxidant Activities of the Essential Oils of Some ...

    African Journals Online (AJOL)

    Purpose: To determine the antimicrobial and antioxidant activities of Pulicaria inuloides and Ocimum forskolei essential oils. Methods: Steam distillation of the aerial parts of P. inuloides and O. forskolei was performed using a Clevenger apparatus. Essential oils were analyzed by gas chromatography–mass spectrometry.

  18. Antioxidant activity of maillard reaction products from lysine-glucose ...

    African Journals Online (AJOL)

    Maillard reaction (MR) was carried out in L-lysine-D-glucose (Lys-Glu) model system heated at 120°C for 0 to 10 h without pH control. Optical property (UV-Vis absorbance and fluorescence) development of MR was monitored. Antioxidant activity of maillard reaction products (MRPs) was investigated by a series of in vitro ...

  19. Seasonal variation in phytochemicals and antioxidant activities in ...

    African Journals Online (AJOL)

    Edoja

    2014-01-22

    Jan 22, 2014 ... Full Length Research Paper. Seasonal variation in phytochemicals and antioxidant activities in different tissues of various Broccoli cultivars. Shiva Ram Bhandari and Jung-Ho Kwak*. Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration,.

  20. Unintended effects were investigated in antioxidant activity between ...

    African Journals Online (AJOL)

    Other than the targeted approach on compositional analysis, non-targeted approaches on genomics, proteomics and metabolomics are developing to search for unintended effects with respect to genetically modified (GM) food safety assessments. Antioxidant activity system was closely related with plant growth and ...

  1. Antioxidant activity of the exudate from Aloe barbadensis leaves in ...

    African Journals Online (AJOL)

    The effects of the exudate of Aloe barbadensis leaves on oxidative stress and some antioxidant status of streptozotocin induced - diabetic rats were studied. There was significant reduction in scavenging enzymes like superoxide dismutase (SOD) activity and significant increase in signs of oxidative tissue damage, such as ...

  2. Retention of phenolics, carotenoids, and antioxidant activity in the ...

    African Journals Online (AJOL)

    The effects of three drying methods on phenolic and carotenoid content and antioxidant activity (AOA) of Tainong 66 cultivar sweet potato (Ipomoea batatas Lam.) were investigated. Roots of 12 batches of freshly harvested tuberous root were washed in tap water, allowed to dry, peeled and cut into small pieces of 0.5 to 1 ...

  3. Extraction and antioxidant activity of phenolic compounds from ...

    African Journals Online (AJOL)

    Purpose: To use response surface methodology to optimize the extraction of the phenolic compounds in wheat bran treated by steam explosion, and to determine the antioxidant activity of the extract obtained. Methods: By using response surface methodology, the effects of extraction time, methanol concentration, ...

  4. Antioxidant Activities of Total Pigment Extract from Blackberries

    Directory of Open Access Journals (Sweden)

    Jiechao Liu

    2005-01-01

    Full Text Available Total pigment has been extracted from blackberries and its antioxidant activity against lipid peroxidation and scavenging capacities towards superoxide anion radicals, hydroxyl radicals and nitrite in different in vitro systems have been investigated. The total pigment extract from blackberries (TPEB exhibited strong antioxidant activity against lipid peroxidation in a linoleic acid model system and scavenging capacities towards superoxide anion radicals, generated by a pyrogallol autoxidation system or by an illuminating riboflavin system, hydroxyl radicals generated by Fenton reaction, and nitrite. Furthermore, the antioxidant activities were correlated with the concentrations of the TPEB. In the test concentration range, the maximum inhibition percentage against linoleic acid peroxidation was 98.32 % after one week’s incubation, and the maximum scavenging percentages for the free radicals and nitrite inhibition in the above reactive systems reached 90.48, 96.48, 93.58 and 98.94 %, respectively. The TPEB is a natural, edible colorant with excellent antioxidant activities and health benefits and it seems to be applicable in both healthy food and medicine.

  5. Antioxidant activity of nebivolol in the rat aorta

    NARCIS (Netherlands)

    de Groot, Annemieke A.; Mathy, Marie-Jeanne; van Zwieten, Pieter A.; Peters, Stephan L. M.

    2004-01-01

    The beta-blocker nebivolol is a racemic mixture Of D- and L-enantiomers that displays negative inotropic as well as direct vasorelaxant activity. In addition, it has been proposed that nebivolol exerts endothelium-protective effects caused by its antioxidant properties. In the present study we

  6. Screening of various botanical extracts for antioxidant activity using ...

    African Journals Online (AJOL)

    Aiming at the exploration of herbal use by society, crude extracts of the seeds of some commonly used medicinal plants (Vitis vinifera, Tamarindus indica and Glycin max) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated ...

  7. Composition and antioxidant and antifungal activities of the ...

    African Journals Online (AJOL)

    In this study, the oil constituents of Lippia gracilis were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The antioxidant and antifungal activities were also evaluated. The leaf oil showed a yield of 3.7% and its main constituents were thymol (70.3%), p-cymene (9.2%), thymol ...

  8. Antioxidant activity, phenolic and flavonoid contents of some wild ...

    African Journals Online (AJOL)

    This study reported on five plants known for their use in traditional medicine in southeastern Algeria, namely: Artemisia campestris, Asteriscus pygmaeus, Pituranthos chlorantus, Pallenis spinosa and Aizoon hispanicum. The work aimed to determine the antioxidant activity, phenol and flavonoid contents of their aqueous ...

  9. total phenols and antioxidant activities of natural honeys

    African Journals Online (AJOL)

    B. S. Chandravanshi

    substance produced by honeybees (Apis mellifera) from the nectar of blossoms and secretions of plants. It is known to have both enzymatic and non-enzymatic antioxidant activities [2, 3]. Though, honey is a highly supersaturated solution of a complex mixture of sugars, it also contains small amount of other constituents ...

  10. Cytotoxic and Antioxidant Activities of Antidesma thwaitesianum Müll ...

    African Journals Online (AJOL)

    Purpose: To investigate the cytotoxic and antioxidant activities of the fruit and fruit waste (residue and marc) extracts of Antidesma thwaitesianum Müll. Arg., known as mamao in Thai, using chemical and cell-based assays. Methods: The cytotoxicity of mamao fruit and fruit waste extracts obtained by expression, maceration ...

  11. Antioxidant activity and total phenolic and flavonoid content of ...

    African Journals Online (AJOL)

    ajl yemi

    2011-12-19

    Dec 19, 2011 ... Full Length Research Paper. Antioxidant activity and total phenolic and flavonoid content of Astragalus squarrosus Bunge. Jinous Asgarpanah1*, Saeed Mohammadi Motamed2, Avishan Farzaneh1, Bahareh. Ghanizadeh1 and Simin Tomraee2. 1Young Researchers Club (YRC), Pharmaceutical Sciences ...

  12. Antioxidant activities of extracts from areca (Areca catectu L.) flower ...

    African Journals Online (AJOL)

    The antioxidant activities of areca ( Areca catectu L.) flower, husk and seed extracts were evaluated using 3 complementary in vitro assays, inhibition of DPPH (1,1-diphenyl-2- picrylhydrazyl) radicals, inhibition of hydroxyl radicals and reducing power system. The EC50 values were calculated for all the methods in order to ...

  13. Antioxidant activity and total phenolic and flavonoid content of ...

    African Journals Online (AJOL)

    The antioxidant capacity of the flowering aerial parts of Astragalus squarrosus was determined by 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferric thiocyanate methods. The phenolic and flavonoid content was also measured. A. squarrosus showed weak free radical scavenging activity with the DPPH ...

  14. Antioxidant and Antimicrobial Activity of Polyphenol Extracts from ...

    African Journals Online (AJOL)

    Purpose: To assess the antioxidant and antimicrobial activities of polyphenolic extracts of three wild red wild berry fruit species from Southeast Serbia, viz, European cornel (Cornus mas), blackthorn (Prunus spinosa L.) and wild blackberry (Rubus fruticosus). Methods: Polyphenol content was determined using ...

  15. Antioxidant activity of the inflorescents of Vernonia calvoana ...

    African Journals Online (AJOL)

    Vernonia calvoana is an indigenous green leafy vegetable that grows around the Cameroons and the southern part of Nigeria. This study was designed to evaluate the phytochemical and antioxidant activity of V. calvoana inflorescents using the following methods: 1, 1 – diphenyl-2-picrylhydrazyl radical (DPPH), Ferric ...

  16. Antioxidant activities of Rosmarinus officinalis L. essential oil ...

    African Journals Online (AJOL)

    The essential oils of Rosmarinus officinalis L. growing in a rural area within the Nkonkobe Municipality of the Eastern Cape, South Africa, were extracted using the solvent free microwave extractor (SFME) and hydro-distillation (HD) methods. The antioxidant and free radical scavenging activity of the obtained oils were tested ...

  17. In vitro antioxidant activities of extracts of Vernonia amygdalina and ...

    African Journals Online (AJOL)

    Vernonia amygdalina and Ocimum gratissimum occupy prominent positions in the diets of several Nigerian ethnic groups. This study therefore compared the phenolic content and some antioxidative activities of the ethanolic leaf extracts of these two plants. Ethanolic extracts of freshly plucked leaves were obtained using ...

  18. Antioxidant and antimicrobial activities of solvent fractions of ...

    African Journals Online (AJOL)

    activity with standard antioxidants, gallic acid and quercetin included in the study. All fractions displayed moderate antimicrobial ..... had reported a similar finding, reporting that fungi are more resistant to plant extracts than ... The study received partial financial support from the Uni- versity of Ibadan Senate research grant ...

  19. Antioxidant activity in vitro and hepatoprotective effects in vivo of ...

    African Journals Online (AJOL)

    Background: Chinese medicine has its own uniqueness, advantageous in the treatment of hepatic diseases, and they were widely used in the oxidation. At the same time, oxidation is one of the mechanism of protect liver. Materials and Methods: In the present study, the antioxidant activity in vitro of different extracts from ...

  20. Alpha-Glucosidase Inhibitory and Antioxidant Activity of Solvent ...

    African Journals Online (AJOL)

    Alpha-Glucosidase Inhibitory and Antioxidant Activity of Solvent Extracts and Fractions of Typha domingensis (Typhaceae) Fruit. Tsun-Thai Chai, Mei-Jee Chiam, Chi-Hou Lau, Nor Ismaliza Mohd Ismail, Hean-Chooi Ong, Fazilah Abd Manan, Fai-Chu Wong ...

  1. Polyphenolic constituents and antioxidant/antiradical activity in ...

    African Journals Online (AJOL)

    Alstonia scholaris (Linn.) leaves extracted in aqueous, dichloromethane (DCM), methanolic and ethanolic solvents were assessed for different polyphenolic constituents endowed with antioxidant/antiradical activity. Total phenolic, flavonoids and tannin contents were significantly (P<0.05) higher in ethanolic extract as ...

  2. Antioxidant and antimicrobial activities of solvent fractions of ...

    African Journals Online (AJOL)

    Results: The ethyl acetate fraction having the IC50 value of 6.50 μg/mL demonstrated comparable DPPH radical-scavenging activity with standard antioxidants, gallic acid and quercetin included in the study. All fractions displayed moderate antimicrobial potential against the tested pathogens with the zone of inhibition that ...

  3. Polyphenolic constituents and antioxidant/antiradical activity in ...

    African Journals Online (AJOL)

    USER

    2015-11-25

    Nov 25, 2015 ... The crushed leaves boiled in the edible oil have been used ... polyphenolic constituents endowed with high antioxidant/ antiradical activity in different extracts of A. scholaris leaves. MATERIALS AND METHODS. Collection and ... container of soxhlet apparatus according to method described by. Harborne ...

  4. Unintended effects were investigated in antioxidant activity between ...

    African Journals Online (AJOL)

    Jane

    2011-08-22

    Aug 22, 2011 ... Other than the targeted approach on compositional analysis, non-targeted approaches on genomics, proteomics and metabolomics are developing to search for unintended effects with respect to genetically modified (GM) food safety assessments. Antioxidant activity system was closely related with.

  5. Determination of antioxidant and α-glucosidase inhibitory activities ...

    African Journals Online (AJOL)

    Antioxidant and α-glucosidase inhibitory activities as well as luteolin contents were analysed in extracts of Chrysanthemum morifolium Ramat (CM), which is a traditional medicinal plant and an important oriental herbal medicine for the treatment of eye diseases in China and Japan. In this study, the results show that the ...

  6. evaluation of antioxidant activity and polyphenolic contents of two ...

    African Journals Online (AJOL)

    A. Djouadi*, T. Lanez and C. Boubekri

    2016-05-01

    May 1, 2016 ... Polyphenolic compounds and antioxidant activity in eggplants (Solanum melongena L.) seem to depend on cultivar, ... two eggplant varieties studied, significant differences in total phenolic content were found in ... In general, the phenolic content of different parts of ethanolic extracts decreased as follows:.

  7. Antioxidant and Anti-proliferative Activities of Flavonoids from ...

    African Journals Online (AJOL)

    Purpose: To investigate the chemical composition of Bidens pilosa L. var. radiata. Sch Bip. (BP), as well as its antioxidant and anti-proliferative activities. Methods: The whole herb of BP was extracted with 95 % ethanol, which was then partitioned sequentially with petroleum ether, ethyl acetate and n-butyl alcohol to obtain ...

  8. Antioxidant activities and flavonoid contents of selected plants ...

    African Journals Online (AJOL)

    user

    2012-09-27

    Sep 27, 2012 ... The methanolic extract of three parasitic plants belonging to family Loranthaceae (Plicosepalus acacia,. Plicosepalus curviflorus and Phragmanthera austro arabica) were investigated for their antioxidant activity. The free radical and nitrogen oxide scavenging abilities were evaluated using diphenyl-1-.

  9. Cytotoxicity, Total Phenolic Contents and Antioxidant Activity of the ...

    African Journals Online (AJOL)

    The leaves of Annona muricata were extracted using ethanol and the extracts were evaluated for cytotoxicity using Brine Shrimp Lethality Assay, total phenolic content (TPC) and antioxidant activity using DPPH radical scavenging assay. The crude extract showed 73.33 % mortality at 1000 μg/mL concentration and its ...

  10. Antioxidant and Antimicrobial Activities of Ethanol Extracts of Cynara ...

    African Journals Online (AJOL)

    Erah

    benefits were due to its high fiber content [2-. 4]. The purpose of this study was to characterize antimicrobial and antioxidant activity of the freeze-dried ethanol extracts of artichoke. Another was to determine its total phenol and flavonoid contents. EXPERIMENTAL. Plant material and its extraction. The dried leaves of Cynara ...

  11. Response of total phenolic content and antioxidant activities of bush

    African Journals Online (AJOL)

    user

    the shade at ambient temperature (Mudau and. Ngezimana, 2014) before being ground with a commercial .... Saturated solution of sodium bicarbonate (20% Na2CO3,. 7.5 ml) was added to the reaction before it was ..... Effects of solvent extraction on phenolic content and antioxidant activity of the by-product of eggplant ...

  12. Effect of Heat on Antioxidant Activity of Some Tropical Leafy ...

    African Journals Online (AJOL)

    USER

    These results suggest that although thermal treatments enhances the nutritional value of vegetables by increasing the bio-accessible phenolic content and total antioxidant activity, long simmer ..... (Odukoya et al., 2007; Morales et al., 2002). Diverse groups of phenolics, being the widest spread secondary metabolite in plant ...

  13. Antioxidant and free radical scavenging activities of plant extracts ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-06-17

    Jun 17, 2008 ... Twenty-two species of medicinal plants collected in the Mexican state of Morelos were selected to evaluate their free radical scavenging and antioxidant activities. The extracts from the aerial parts of the plants were obtained using hexane, acetone and methanol (66 extracts). The initial qualitative screening.

  14. Antioxidant activities of the selected plants from the family ...

    African Journals Online (AJOL)

    USER

    2010-02-15

    Feb 15, 2010 ... were found in n-butanol extract of Ricinus communis. Antioxidant activities of these extracts were evaluated through DPPH• radical scavenging, phosphomolybdate and ferric thiocyanate (FTC) methods. Methanolic extract of Cinnamomum zeylanicum and Cinnamomum tamala showed highest antiradical.

  15. Observation of antioxidant activity of leaves, callus and suspension ...

    African Journals Online (AJOL)

    Antioxidant activity and phenolic compound was found in Justicia gendarussa via total phenolic content (TPC) and ,-diphenyl--pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture of J. gendarussa. Callus ...

  16. Antioxidant activity of aqueous extract of a Tolypocladium sp ...

    African Journals Online (AJOL)

    Tolypocladium sp. Ts-1 was isolated from the fruiting body of a wild Cordyceps sinensis, one of the best known traditional Chinese medicine and health foods. The antioxidant activities of hot-water extracts from cultured mycelia of Tolypocladium sp. were assessed in different in vitro systems. The extracts showed ...

  17. Antioxidant activity of aqueous extract of a Tolypocladium sp. fungus ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-09-03

    Sep 3, 2008 ... Tolypocladium sp. Ts-1 was isolated from the fruiting body of a wild Cordyceps sinensis, one of the best known traditional Chinese medicine and health foods. The antioxidant activities of hot-water extracts from cultured mycelia of Tolypocladium sp. were assessed in different in vitro systems. The extracts ...

  18. Improvement of nutritional quality and antioxidant activities of yeast ...

    African Journals Online (AJOL)

    Jane

    2011-06-27

    Jun 27, 2011 ... the nutritional quality and antioxidant activities of the substrate were enhanced by solid yeast treatment fermentation. Thus, scope exists for ..... may have definite advantages in a diet. It can act as a suitable replacement ..... varying degrees, while giving a good performance in its scavenging of free radicals.

  19. Antioxidant, anti-inflammatory and antinociceptive activities of ...

    African Journals Online (AJOL)

    Background of study: Plants used for traditional medicine contain a wide range of substances which can be used to treat various infectious diseases. Aim: The study evaluated the in vitro antioxidant, antinociceptive, and anti-inflammatory activities of the methanolic extract of Justicia secunda Vahl leaf. Methods: The acute ...

  20. Fulvic acid affects pepper antioxidant activity and fruit quality

    African Journals Online (AJOL)

    standard

    2012-08-23

    Aug 23, 2012 ... analysis). Methanol extracts of freeze-dried fruits were prepared for the determination of antioxidant activity and total phenolic content. Weighed pepper fruit samples (5 g) were placed in a glass beaker and homogenised with 50 ml of methanol at. 24°C overnight. The homogenate was filtered and then.

  1. Correlation of antioxidant activity of dried berry infusions with the ...

    African Journals Online (AJOL)

    Antioxidant activity was measured by ferric reducing ability of plasma (FRAP) assay in seven types of infusions prepared from commercial dried berry fruit products: Rosa canina, Vaccinium vitis-idaea, Hiphophae rhamnoides, Hibiscus sabdariffa and three fruit mixtures. Total polyphenols (TP), total anthocyanins and the ...

  2. A novel acyclic diterpenic alcohol isolated from antioxidant active ...

    African Journals Online (AJOL)

    Background: Isolation and characterization of a new compound from the antioxidant active ethanol extract of leaves of an endemic plant Centaurothamnus maximus. Methods: The air dried powdered leaves of the plant was extracted successively with n-hexane, dichloromethane, ethyl acetate and ethanol. The obtained ...

  3. Antioxidant Activities of the Leaf Extract and Fractions of Cola ...

    African Journals Online (AJOL)

    Prof. Ogunji

    the possibility of chronic diseases such as diabetes, cancer and cardiovascular diseases (Myojin et al.,. 2008; Saha et al., 2004; Horax et al., 2005; Semiz and Sen, .... in 4 varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts. J. Food. Sci. 70(4): 275-280. Ito, N., Fukushima, S., Hagiwara, ...

  4. Application of metabolite profiling and antioxidant activity in ...

    African Journals Online (AJOL)

    This paper investigates the effect of processing and extraction method on chemical profiles and antioxidant activity of Malaysian stingless bee propolis. High Performance Thin Layer Chromatography (HPTLC) analysis in combination with chemometric shows that some of the compounds were degraded or not detected in ...

  5. Synthesis and evaluation of antioxidant and antimicrobial activities ...

    African Journals Online (AJOL)

    Purpose: To synthesize and evaluate Schiff base Tin (II) complexes for antioxidant and antimicrobial activities. Methods: The complexes of Tin (II) chloride with various Schiff base derivative of 2-Hydroxy-1- naphthaldehyde (HN) were synthesized and characterized by various physiochemical techniques, including elemental ...

  6. In vitro Antifungal, Antioxidant and Cytotoxic Activities of a Partially ...

    African Journals Online (AJOL)

    Purpose: To determine the in vitro antifungal and antioxidant activities of the aqueous extract and protein fraction of Atlantia monophylla Linn (Rutaceae) leaf. Methods: Ammonium sulphate (0 – 80 %) precipitation method was used to extract protein from the leaves of A. monophylla Linn (Rutaceae). In vitro antifungal ...

  7. In vitro callus induction and antioxidant activity of Rauwolfia ...

    African Journals Online (AJOL)

    Background: Rauwolfia vomitoria Afzel. (Apocynaceae) is a medicinal plant valued for its antipsychotic effect and used in various herbal preparations. Objectives: This study was designed to develop a protocol for callus initiation in Rauvolfia vomitoria and to investigate the antioxidant activity of the wild plant and the ...

  8. Major factors influencing antioxidant contents and antioxidant activity in grapes and wines

    Directory of Open Access Journals (Sweden)

    Jaromír Lachman

    2009-03-01

    Full Text Available Jaromír Lachman, Miloslav Šulc, Katerina Faitová, Vladimír PivecDepartment of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicAbstract: Phenolic compounds in wines, especially in red wines, possess strong antioxidant activity, have the largest effect in decreasing atherosclerosis by both hypolipemic and antioxidant mechanisms. The long-term uptake of red wine has a positive impact on antioxidant activity (AA of blood plasma in rats in vivo and increases AA by 15%–20% compared to a control group. In the article the effect of total phenolics (TP, total anthocyanins (TA, individual anthocyanins, procyanidins and phenolics contained in red grapes, musts, grape seeds and skins and wines on the AA is discussed. Significant impact of varieties, viticultural regions and locations, climate conditions and vintage has been shown. Likewise, the ways and individual stages of the vinification technology process, and storage conditions affect color, TP, TA, and AA and health aspects of produced wines. Resveratrol, another free radical scavenger mainly contained in the skins of grapes, inhibits the risk of cardiovascular diseases. Higher amounts of trans-resveratrol (RES have been found in wines from cool and wet climate regions and lesser amounts are typical for warm and dry regions. Changes in the TP content and AA affected by grape variety, vineyard location and winemaking process in white and blue varieties from different vineyards of the Czech Republic were studied. Significant differences in TP among varieties were found. Analysis of variance showed statistically high differences among red and white wines and growing locations. Wines differed significantly in TP content and AA increased significantly during the winemaking process. Statistically significant differences in AA values were found among growing areas, wines and varieties. Significant positive correlations between TP

  9. Preparation of antioxidant peptide from egg white protein and improvement of its activities assisted by high-intensity pulsed electric field.

    Science.gov (United States)

    Lin, Songyi; Guo, Yang; You, Qi; Yin, Yongguang; Liu, Jingbo

    2012-05-01

    Egg white protein powder was hydrolyzed by three proteases-alcalase, trypsin, and pepsin-to produce antioxidant peptides. Three kinds of hydrolysates were prepared under optimal enzymatic parameters that were obtained from the preliminary one-factor-at-a-time test and response surface methodology. Thereafter, three enzymatic hydrolysates were sequentially fractionated by ultrafiltration membranes in cut-off MW of 30, 10, and 1 kDa, and tested in terms of their reducing power (RP). Effects of high-intensity pulsed electric field (PEF) were further investigated on the antioxidant peptides to improve their activities. Alcalase hydrolysates possessed stronger RP ability than the other two hydrolysates, particularly for the fraction within alcalase could be regarded as the most appropriate enzyme for preparation of bioactive peptide from egg white protein, with the best antioxidant activity. Also, PEF showed some effects on the peptide and could be further applied to improve its antioxidant activity. Copyright © 2011 Society of Chemical Industry.

  10. Chemical composition, antioxidant properties and antimicrobial activity of the essential oil of Murraya paniculata leaves from the mountains of Central Cuba.

    Science.gov (United States)

    Rodríguez, Elisa Jorge; Ramis-Ramos, Guillermo; Heyden, Yvan Vander; Simó-Alfonso, Ernesto F; Lerma-García, María Jesús; Saucedo-Hernández, Yanelis; Monteagudo, Urbano; Morales, Yeni; Holgado, Beatriz; Herrero-Martínez, José Manuel

    2012-11-01

    The essential oil of Murraya paniculata L leaves from the mountains of the Central Region of Cuba, obtained by hydrodistillation, was analyzed by gas chromatography-mass spectrometry. Eighteen compounds, accounting for 95.1% of the oil were identified. The major component was beta-caryophyllene (ca. 30%). The antioxidant activity of essential oil was evaluated against Cucurbita seed oil by peroxide, thiobarbituric acid and p-anisidine methods. The essential oil showed stronger antioxidant activity than that of butylated hydroxyanisole and butylated hydroxytoluene, but lower than that of propyl gallate. Moreover, this antioxidant activity was supported by the complementary antioxidant assay in the linoleic acid system and 2, 2'-diphenyl-1-picrylhydrazyl. The essential oil also showed good to moderate inhibitory effects against Klebsiellapneumoniae and Bacillus subtilis.

  11. Investigation of antioxidant activity and lipophilicity parameters of some preservatives

    Energy Technology Data Exchange (ETDEWEB)

    Korotkova, E.I. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation) and Scientific Research Center ' Polyant' , Kulagina 5-29, Tomsk (Russian Federation)]. E-mail: eikor@mail.ru; Avramchik, O.A. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation); Scientific Research Center ' Polyant' , Kulagina 5-29, Tomsk (Russian Federation); Angelov, T.M. [Department of Quality Control, Unipharm-JSCo, Traiko Stanoev 3, Sofia (Bulgaria); Karbainov, Y.A. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation)

    2005-10-10

    The preservatives have been widely used in food, drugs and cosmetic products to prevent their aging and decay. Antioxidant and antimicrobial activities of some preservatives, such as benzoic acid, sorbic acid and alkyl-parabens, were considered in this work. Study of antioxidant properties of these compounds was carried out by differential pulse voltammetry together with evaluation of their influence on the kinetics of the electrochemical oxygen reduction. Mathematical models of the processes were obtained and their adequacy was estimated using design of experiment methods. As a result, the kinetic parameters of interaction between reactive oxygen species and preservatives were evaluation. Finally, the use of these substances as antimicrobials and antioxidants ensured product stability and safety has been recommended.

  12. Antioxidative, anticholinesterase and antityrosinase activities of the ...

    African Journals Online (AJOL)

    The solvent extracts of a red alga Grateloupia lancifolia were used to evaluate biofunctional activities. The diethyl ether extract showed the highest total phenolic content and biological activities. The IC50 value of the diethyl ether extract on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was 113.8 μg/ml.

  13. Antioxidant activities of the constituents of Picris echoides

    Directory of Open Access Journals (Sweden)

    MILUTIN STEFANOVIC

    2002-01-01

    Full Text Available Some flavonoids with antioxidant properties from the aerial parts of the plant species Picris echoides (family Asteraceae were identified. Upon chromatography, the ethyl acetate extract afforded flavonoids, such as: flavone apigenin (1 and its glucoside, cosmosiin (2, as well as common plant constituents from this family, flavonol galetin (3, 3,4’,5,6,7-pentahydroxyflavone and 4,4’,6,7-tetrahydroxyaurone (4. The structure of the aurone 4 has not been described so far in the literature and presented a very rare type of aurone skeleton. The structures of the isolated compounds were determined by interpretation of their physical and spectral data. The antioxidant activities of different extracts from Picris echoides were measured by the Schaal oven test at 60ºC and by the Rancimat method at 100ºC. Water/ethanol extracts (2:8, v/v, in concentrations of 0.02 and 0.05 %, showed lower activity than commercial tocopherol (Tch. On the contrary, the purified ethyl acetate extracts showed a strong concentration-dependent antioxidant effect. The investigation demonstrated that galetin was the main flavonol from this origin. According to the results of the two methods, galetin (3 showed a two-fold better activity than did Tch and a lower activity than did butylated hydroxyanisole (BHA. The aurone 4 exhibited significantly lower antioxidant activity than did galetin at the same concentration level. Thus, the plant species P. echoides is a new and favorable source of natural lipid antioxidants.

  14. Antioxidant activity of extract from Polygonum aviculare L.

    Directory of Open Access Journals (Sweden)

    CHIN-YUAN HSU

    2006-01-01

    Full Text Available Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum aviculare L. (Polygonaceae has antioxidant activity is unknown. In this study, dried Polygonum aviculare L. was extracted by ethanol, and the extract was lyophilized. The antioxidant activities of extract powder were examined by free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays. The results show that the IC50 value of Polygonum aviculare L. extract is 50 µg/ml in free radical scavenging assays, 0.8 µg/ml in superoxide radical scavenging assays, and 15 µg/ml in lipid peroxidation assays, respectively. Furthermore, Polygonum aviculare L. extract has DNA protective effect in hydroxyl radical-induced DNA strand scission assays. The total phenolics and flavonoid content of extract is 677.4 ± 62.7 µg/g and 112.7 ± 13 µg/g. The results indicate that Polygonum aviculare L. extract clearly has antioxidant effects

  15. Antigenotoxic and antioxidant activities of palmarosa and citronella essential oils.

    Science.gov (United States)

    Sinha, Sonali; Biswas, Dhrubojyoti; Mukherjee, Anita

    2011-10-11

    Essential oils of palmarosa and citronella have been extensively used in ancient Indian and South-east Asian traditional medicines. These essential oils have been reported to exhibit antimicrobial, anti parasitic effects against bacteria, yeasts, filamentous fungi, and viruses. In the present study the oils were tested for their potential antigenotoxic and antioxidant properties in human lymphocyte cells. The antigenotoxic effect on human lymphocyte cells (measurement of cell viability, DNA damage) was studied using trypan blue dye exclusion test, plasmid pBR322 DNA strand scission, and comet assay. Anti-oxidant activity was evaluated by DPPH(+) free radical scavenging and lipid peroxidation assay. The essential oils showed a good antigenotoxic activity against methyl methanesulphonate (MMS) and hydrogen peroxide. In addition, a significant dose dependent antioxidant activity was observed. Our data provide evidence that support the usage of palmarosa and citronella essential oils in traditional herbal preparations. They can constitute a natural source of a new and safe antioxidant. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  16. Phenolic acids and antioxidant activity of wheat species: a review

    Directory of Open Access Journals (Sweden)

    Leváková Ľudmila

    2017-10-01

    Full Text Available Wheat (genus Triticum is considered to be an important source of polyphenols, plant secondary metabolites with numerous health-promoting effects. Many phytochemicals are responsible for the high antioxidant activity of whole grain products. However, there is a lack of information about composition of phenolic acids and their concentrations in different Triticum species. Despite the fact that the increased consumption of whole grain cereals and whole grain-based products has been closely related to reduced risk of chronic diseases, bioactive compounds found in whole grain cereals have not achieved as much attention as the bioactive compounds in vegetables and fruits. Recent studies have revealed that the content of bioactive compounds and antioxidant capacity of whole grain cereals have been regularly undervalued in the literature, because they contain more polyphenols and other phytochemicals than was reported in the past. Phenolic acids represent a large group of bioactive compounds in cereals. These compounds play a significant role in the possible positive effects of the human diet rich in whole grain cereals, especially in wheat and provide health benefits associated with demonstrably diminished risk of chronic disease development. Ferulic acid, the primary and the most abundant phenolic acid contained in wheat grain, is mainly responsible for the antioxidant activity of wheat, particularly bran fraction. In this paper, selected phenolic compounds in wheat, their antioxidant activity and health benefits related to consumption of whole grain cereals are reviewed.

  17. Anticancer and Antioxidant Activity of Bread Enriched with Broccoli Sprouts

    Science.gov (United States)

    Gawlik-Dziki, Urszula; Świeca, Michał; Dziki, Dariusz; Sęczyk, Łukasz; Złotek, Urszula; Różyło, Renata; Kaszuba, Kinga; Ryszawy, Damian; Czyż, Jarosław

    2014-01-01

    This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS) in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities); however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS) was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention. PMID:25050366

  18. Fatty Acids, Antioxidants and Physical Activity in Brain Aging

    Directory of Open Access Journals (Sweden)

    Hércules Rezende Freitas

    2017-11-01

    Full Text Available Polyunsaturated fatty acids and antioxidants are important mediators in the central nervous system. Lipid derivatives may control the production of proinflammatory agents and regulate NF-κB activity, microglial activation, and fatty acid oxidation; on the other hand, antioxidants, such as glutathione and ascorbate, have been shown to signal through transmitter receptors and protect against acute and chronic oxidative stress, modulating the activity of different signaling pathways. Several authors have investigated the role of these nutrients in the brains of the young and the aged in degenerative diseases such as Alzheimer’s and Parkinson’s, and during brain aging due to adiposity- and physical inactivity-mediated metabolic disturbances, chronic inflammation, and oxidative stress. Through a literature review, we aimed to highlight recent data on the role of adiposity, fatty acids, antioxidants, and physical inactivity in the pathophysiology of the brain and in the molecular mechanisms of senescence. Data indicate the complexity and necessity of endogenous/dietary antioxidants for the maintenance of redox status and the control of neuroglial signaling under stress. Recent studies also indicate that omega-3 and -6 fatty acids act in a competitive manner to generate mediators for energy metabolism, influencing feeding behavior, neural plasticity, and memory during aging. Finding pharmacological or dietary resources that mitigate or prevent neurodegenerative affections continues to be a great challenge and requires additional effort from researchers, clinicians, and nutritionists in the field.

  19. Anticancer and antioxidant activity of bread enriched with broccoli sprouts.

    Science.gov (United States)

    Gawlik-Dziki, Urszula; Świeca, Michał; Dziki, Dariusz; Sęczyk, Łukasz; Złotek, Urszula; Różyło, Renata; Kaszuba, Kinga; Ryszawy, Damian; Czyż, Jarosław

    2014-01-01

    This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS) in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities); however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS) was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention.

  20. Volatile compounds and antioxidative activity of Porophyllum tagetoides extracts.

    Science.gov (United States)

    Jimenez, M; Guzman, A P; Azuara, E; Garcia, O; Mendoza, M R; Beristain, C I

    2012-03-01

    Porophyllum tagetoides is an annual warm-weather herb that has an intense typical smell. Its leaves are commonly used in soup preparation and traditional medicine for treatment of inflammatory diseases. Its volatile compounds and antioxidant properties were evaluated in crude, aqueous and ethanol leaf extract and an oil emulsion using different antioxidant assays in vitro, such as: DPPH radical scavenging activity, redox potential, polyphenol content, reducing power and optical density. A high antioxidative activity was found when comparing leaves with stems. The crude extract from leaves showed a very high reducing power (2.88 ± 0.20 O.D.) and DPPH radical-scavenging activity (54.63 ± 4.80%), in concordance with a major concentration of vitamin C (23.97 ± 0.36 mg/100 g). Instead, the highest polyphenol content (264.54 ± 2.17 mg GAE/g of sample) and redox potential (561.23 ± 0.15 mV) were found by the ethanol and aqueous extract, respectively. Aldehydes and terpenes such as nonanal, decanal, trans-pineno, β-myrcene and D-limonene were the major volatiles found. This study suggests that Porophyllum tagetoides extracts could be used as antioxidants.

  1. Anticancer and Antioxidant Activity of Bread Enriched with Broccoli Sprouts

    Directory of Open Access Journals (Sweden)

    Urszula Gawlik-Dziki

    2014-01-01

    Full Text Available This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities; however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention.

  2. Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extracts.

    Science.gov (United States)

    Karasu, Cimen; Cumaoğlu, Ahmet; Gürpinar, Ali Rifat; Kartal, Murat; Kovacikova, Lucia; Milackova, Ivana; Stefek, Milan

    2012-03-01

    The pomegranate, Punica granatum L., has been the subject of current interest as a medicinal agent with wide-ranging therapeutic indications. In the present study, pomegranate ethanolic seed and hull extracts were tested, in comparison with a commercial sample, for the inhibition of aldose reductase, an enzyme involved in the etiology of diabetic complications. In vitro inhibition of rat lens aldose reductase was determined by a conventional method. Pomegranate ethanolic hull extract and commercial pomegranate hull extract exhibited similar aldose reductase inhibitory activity characterized by IC(50) values ranging from 3 to 33.3 μg/ml. They were more effective than pomegranate ethanolic seed extract with IC(50) ranging from 33.3 to 333 μg/ml. Antioxidant action of the novel compounds was documented in a DPPH test and in a liposomal membrane model, oxidatively stressed by peroxyl radicals. All the plant extracts showed considerable antioxidant potential in the DPPH assay. Pomegranate ethanolic hull extract and commercial pomegranate hull extract executed similar protective effects on peroxidatively damaged liposomal membranes characterized by 10ethanolic seed extract showed significantly lower antioxidant activity compared to both hull extracts studied. Pomegranate extracts are thus presented as bifunctional agents combining aldose reductase inhibitory action with antioxidant activity and with potential therapeutic use in prevention of diabetic complications.

  3. Total antioxidant activity of yacon tubers cultivated in Brazil

    Directory of Open Access Journals (Sweden)

    Juciane Abreu Ribeiro Pereira

    Full Text Available ABSTRACT Yacon (Smallanthus sonchifolius is a tuberous root from the Andean region in the South America rich em water, fructooligosaccharides and phenolic compounds, some of which are natural antioxidants and may help prevent the deleterious action of free radicals in the body. The yacon has attracted much attention due to their potential health benefits to humans. In this study the levels of total phenolics, tannins, phenolic acids, and total antioxidant activity were measured in the peel and pulp of yacon tubers both in the fresh and flour forms. The flours of yacon presented higher concentrations of total phenolics and tannins, especially peel flour. The yacon pulp flour stood out as the main source of phenolic acids, mainly caffeic and chlorogenic acid. The total antioxidant activity assessed by DPPH (2,2-Diphenyl-1-picrylhydrazyl and ABTS (2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid assays was higher in the yacon peel flour. The total antioxidant activity was correlated with the total phenolic content and tannins by the DPPH and ABTS assays. These results suggest that yacon can be used as an alternative food source of phenolic compounds that help prevent degenerative processes caused by oxidative stress, especially in the flours form.

  4. Phytochemical Profiles and Antioxidant and Antimicrobial Activities of the Leaves of Zanthoxylum bungeanum

    Science.gov (United States)

    Luo, Ziwen; Wang, Dongmei; He, Fengyuan; Li, Dengwu

    2014-01-01

    The ethanol crude extracts (ECE) and their subfractions from Zanthoxylum bungeanum leaves were prepared and their phytochemical profiles and antioxidant and antimicrobial activities were investigated. Moreover, the effective HPLC procedure for simultaneous quantification of twelve compounds in Z. bungeanum leaves was established. The correlation between the phytochemicals and antioxidant activity was also discussed. The ethyl acetate fraction (EAF) had the highest total phenolic (97.29 mmol GAE/100 g) and flavonoid content (67.93 mmol QE/100 g), while the greatest total alkaloid content (4.39 mmol GAE/100 g) was observed in the chloroform fraction (CF). Twelve compounds were quantified by RP-HPLC assay. EAF exhibited the highest content of quercitrin, kaempferol-3-rhamnoside, quercetin, sesamin, and nitidine chloride (125.21, 54.95, 24.36, 26.24, and 0.20 mg/g); acetone fraction (AF) contained the highest content of chlorogenic acid, rutin, hyperoside, and trifolin (5.87, 29.94, 98.33, and 31.24 mg/g), while kaempferol-3-rhamnoside, xanthyletin, and sesamin were rich in CF. EAF and AF exhibited significant DPPH, ABTS radical scavenging abilities and reducing power (FRAP), whereas CF exhibited significant antifungal activity. Moreover, EAF also showed stronger antibacterial activity. In conclusion, Z. bungeanum leaves have health benefits when consumed and could be served as an accessible source for production of functional food ingredients and medicinal exploration. PMID:25147836

  5. Phytochemical Profiles and Antioxidant and Antimicrobial Activities of the Leaves of Zanthoxylum bungeanum

    Directory of Open Access Journals (Sweden)

    Yujuan Zhang

    2014-01-01

    Full Text Available The ethanol crude extracts (ECE and their subfractions from Zanthoxylum bungeanum leaves were prepared and their phytochemical profiles and antioxidant and antimicrobial activities were investigated. Moreover, the effective HPLC procedure for simultaneous quantification of twelve compounds in Z. bungeanum leaves was established. The correlation between the phytochemicals and antioxidant activity was also discussed. The ethyl acetate fraction (EAF had the highest total phenolic (97.29 mmol GAE/100 g and flavonoid content (67.93 mmol QE/100 g, while the greatest total alkaloid content (4.39 mmol GAE/100 g was observed in the chloroform fraction (CF. Twelve compounds were quantified by RP-HPLC assay. EAF exhibited the highest content of quercitrin, kaempferol-3-rhamnoside, quercetin, sesamin, and nitidine chloride (125.21, 54.95, 24.36, 26.24, and 0.20 mg/g; acetone fraction (AF contained the highest content of chlorogenic acid, rutin, hyperoside, and trifolin (5.87, 29.94, 98.33, and 31.24 mg/g, while kaempferol-3-rhamnoside, xanthyletin, and sesamin were rich in CF. EAF and AF exhibited significant DPPH, ABTS radical scavenging abilities and reducing power (FRAP, whereas CF exhibited significant antifungal activity. Moreover, EAF also showed stronger antibacterial activity. In conclusion, Z. bungeanum leaves have health benefits when consumed and could be served as an accessible source for production of functional food ingredients and medicinal exploration.

  6. Antioxidant activities of orange peel extract in ghee (butter oil) stored at different storage temperatures.

    Science.gov (United States)

    Asha, A; Manjunatha, M; Rekha, R M; Surendranath, B; Heartwin, P; Rao, J; Magdaline, E; Sinha, Chitranayak

    2015-12-01

    Antioxidant activities of butylatedhydroxyanisole (BHA) and orange peel powder extract in ghee stored at different storage temperatures (T1:6 ± 2 °C; T2: 32 ± 2 °C; T3:60 ± 2 °C) were evaluated during storage period of 21 days. Peroxide value (PV), thiobarbituric acid (TBA), radical scavenging activity (RSA) and free fatty acids (FFA) of ghee samples were analyzed during the study. PV, TBA and FFA of ghee samples increased significantly while radical scavenging activity (RSA) of ghee samples decreased significantly at accelerated temperature (T3) as compared to the temperatures at T1 and T2. Effect of storage temperature on development of peroxides and TBA of ghee samples was significantly higher than the effect of treatment and storage period while treatment had more significant effect on the change in FFA and RSA as compared to storage temperature and storage period. Ghee incorporated with orange peel extract (OPE) showed stronger activity in quenching DPPH radicals and least development of PV, TBA and FFA than ghee incorporated with BHA and control. The study revealed that orange peel could be a good natural source of antioxidants which can be used in fat rich food products like ghee to retard oxidative deterioration.

  7. Antioxidant activity of the probiotic consortium in vitro

    Directory of Open Access Journals (Sweden)

    Saule Saduakhasova

    2014-01-01

    Full Text Available Introduction: Available evidence suggests that probiotics have different biological functions that depend on several mechanisms, such as antioxidant and DNA-protective activities. The probiotic consortium includes bacterial cultures such as Streptococcus thermophilus, Lactococcus lactis, Lactobacillus plantarum, and other bacterial cultures isolated from traditional Kazakh dairy products (ayran, kumys, shubat, and healthy clinical material. The aim of this study was to investigate the total antioxidant activity of the consortium of probiotic bacteria and to determine the activity of superoxide dismutase, glutathione reductase, and DNA-protective action. Material and methods: In vitro comet assay was used to determine the antigenotoxicity of the probiotic consortium. Total antioxidant activity was determined using a method of analysis with Trolox as the equivalent. The analysis method of superoxide dismutase activity assesses the inhibition rate of the nitroblue tetrazolium reduction to formazan by superoxide dismutase. Determination of glutathione reductase activity is based on the measurement of the NADPH oxidation speed. Results: A significantly high level of the total antioxidant activity of the probiotic consortium intact cells (15.3 mM/ml was observed whereas the activity index of  lysate  was 11.1 mM/ml. The superoxide dismutase activity of probiotic consortium lysate was evaluated, with values that peaked at 0.24 U/mg protein. The superoxide dismutase activity of the consortium was lower in comparison to L.fernentum E-3 and L.fernentum E-18 cultures with values of 0.85 U/mg and 0.76 U/mg protein, respectively. SOD activity of probiotic consortium whole cells was not observed, which is typical for lactic acid bacteria. Glutathione reductase plays an important role in the optimal protection from oxidative stress. Glutathione reductase activity of the studied probiotic consortium was low; moreover, the activity of the lysate was two times

  8. Antioxidant activity and irritation property of venoms from Apis species.

    Science.gov (United States)

    Somwongin, Suvimol; Chantawannakul, Panuwan; Chaiyana, Wantida

    2018-04-01

    Pharmacological effects of bee venom has been reported, however, it has been restricted to the bee venom collected from European honey bee (Apis mellifera). The aim of the present study was to compare the antioxidant activities and irritation properties of venoms collected from four different Apis species in Thailand, which includes Apis cerena (Asian cavity nesting honeybee), Apis florea (dwarf honeybee), Apis dorsata (giant honeybee), and A. mellifera. Melittin content of each bee venom extracts was investigated by using high-performance liquid chromatography. Ferric reducing antioxidant power, 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid), and 1, 1-diphenyl-2-picrylhydrazyl assay were used to determine the antioxidant activity, whereas, hen's egg test chorioallantoic membrane assay was used to determine the irritation property of each bee venom extracts. Melittin was the major constituent in all bee venom extracts. The melittin content in A. dorsata, A. mellifera, A. florea, and A. cerena were 95.8 ± 3.2%, 76.5 ± 1.9%, 66.3 ± 8.6%, and 56.8 ± 1.8%, respectively. Bee venom extract from A. dorsata possessed the highest antioxidant activity with the inhibition of 41.1 ± 2.2% against DPPH, Trolox equivalent antioxidant capacity of 10.21 ± 0.74 mM Trolox/mg and equivalent concentration (EC 1 ) of 0.35 ± 0.02 mM FeSO 4 /mg. Bee venom extract from A. mellifera exhibited the highest irritation, followed by A. cerena, A. dorsata, and A. florea, respectively. Melittin was the compound responsible for the irritation property of bee venom extracts since it could induce severe irritation (irritation score was 13.7 ± 0.5, at the concentration of 2 mg/ml). The extract from A. dorsata which possessed the highest antioxidant activity showed no irritation up to the concentration of 0.1 mg/ml. Therefore, bee venom extract from A. dorsata at the concentration not more than 0.1 mg/ml would be suggested for using

  9. Antioxidant, Antitubercular and Cytotoxic Activities of Piper imperiale

    Directory of Open Access Journals (Sweden)

    Sanjib Bhakta

    2012-04-01

    Full Text Available Phenolic compounds are widely distributed in Nature and act as pharmacologically active constituents in many herbal medicines. They have multiple biological properties, most notably antioxidant, antibacterial and cytotoxic activities. In the present study an attempt to correlate the phenolic composition of leaf, flower and wood extracts of Piper imperiale, with antioxidant, antitubercular and cytotoxic activities was undertaken. The total phenol content ranged from 1.98 to 6.94 mg GAE/gDW among ethanolic extracts, and gallic acid, catechin, epicatechin, ferulic acid, resveratrol and quercetin were identified and quantified by HPLC. DPPH and ABTS assays showed high antioxidant activity of the leaf extract (EC50ABTS = 15.6 µg/mL, EC50DPPH = 27.3 µg/mL with EC50 in the same order of magnitude as the hydroxyquinone (EC50ABTS = 10.2 µg/mL, EC50DPPH = 15.7 µg/mL. The flower extract showed strong antimicrobial activity against Mycobacterium tuberculosis H37Rv. All the extracts exhibited dose-dependent cytotoxic effects against MCF-7 cancer cells. This is the first time that a Piper extract has been found to be highly active against M. tuberculosis. This study shows the biological potential of Piper imperiale extracts and gives way to bio-guided studies with well-defined biological activities.

  10. Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

    Science.gov (United States)

    Rosli, Nur Liyana; Roslan, Husniyati; Omar, Eshaifol Azam; Mokhtar, Norehan; Hapit, Nor Hussaini Abdul; Asem, Nornaimah

    2016-12-01

    Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyze the phytochemical profile of propolis derived from local T. apicalis species and its antioxidant activities. The ethanolic extract of propolis was subjected to HPLC analysis to analyze its phytochemical profile. The propolis extract was later tested for antioxidant capacities by using DPPH radical scavenging assay. TPC and TFC were performed to determine the correlation with its antioxidant activities. TEAC for each serial dilution sample was 2621.15 (4.76 mg/mL), 2050.85 (2.38 mg/mL), 1883.27 (1.19 mg/mL), 1562.67 (0.59 mg/mL), 1327.82 (0.29 mg/mL), 1164.49 (0.15 mg/mL), 983.27 (0.07 mg/mL), and 944.79 (0.04 mg/mL). The results demonstrated that the antioxidant activities of propolis extract were dose dependent. The IC50 of propolis for DPPH assay was 4.27 mg/ml. Correlation values of TPC and TFC against DPPH indicate that the antioxidant activities of propolis extract used in this study could be mainly influenced by the phenolic and flavonoid contents. These findings highlighted the importance of quality analysis in order to ensure the consistency of biological effects or therapy of a natural product, such as propolis.

  11. Essential oil of Psidium cattleianum leaves: antioxidant and antifungal activity.

    Science.gov (United States)

    Castro, Micheli R; Victoria, Francine N; Oliveira, Daniela H; Jacob, Raquel G; Savegnago, Lucielli; Alves, Diego

    2015-02-01

    Psidium cattleianum Sabine (Myrtacea) is rich in vitamin C and phenolic compounds, including epicatechin and gallic acid as the main components. To evaluate the antifungal and antioxidant capacity in vitro of the essential oil of araçá (EOA). The acute toxicity of the EOA also was evaluated in mice. The leaves of the P. cattleianum were extracted by steam distillation. The antioxidant capacity was evaluated by in vitro tests [1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS), ferric ion reducing antioxidant power (FRAP), linoleic acid oxidation, thiobarbituric acid reactive species (TBARS)], and ex vivo analysis [TBARS, δ-aminulevunilate dehydratase (δ-Ala-D) and catalase activity, non-protein thiols (NPSH), and ascorbic acid levels]. The toxicity was studied in mice by a single oral administration of EOA; and the antifungal activity was performed with five strains of fungi. The EOA exhibited antioxidant activity in the FRAP assay and reduced lipid peroxidation in the cortex (Imax = 32.90 ± 2.62%), hippocampus (IC50 = 48.00 ± 3.00 µg/ml and Imax = 32.90 ± 2.62%), and cerebellum (Imax = 45.40 ± 14.04%) of mice. Acute administration of the EOA by the oral route did not cause toxicological effects in mice (LD50 > 500 µg/ml). The EOA also showed antifungal activity through of the determination minimum inhibitory concentration (MIC) values ranging from 41.67 ± 18.04 to 166.70 ± 72.17 µg/ml for tested strains. The results of present study indicate that EOA possess antioxidant properties, antifungal and not cause toxicity at tested doses.

  12. Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.

    Science.gov (United States)

    Pittella, Frederico; Dutra, Rafael C.; Junior, Dalton D.; Lopes, Miriam T. P.; Barbosa, Nádia R.

    2009-01-01

    In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC50 value of 31.25 μg/mL. The AE had a promising activity against mouse melanoma (B16F1), human breast cancer (MDA MB-231) and rat glioma (C6) cell lines, with IC50 values of 698.0, 648.0 and 1000.0 μg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities. PMID:19865514

  13. Antioxidant and Cytotoxic Activities of Centella asiatica (L Urb.

    Directory of Open Access Journals (Sweden)

    Frederico Pittella

    2009-08-01

    Full Text Available In the present study, the phenolic (Folin-Dennis and flavonoid (colorimetric assay constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH assay] and cytotoxic activities of an aqueous extract (AE of Centella asiatica leaves were investigated. The aqueous extract (50 g/L was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC50 value of 31.25 μg/mL. The AE had a promising activity against mouse melanoma (B16F1, human breast cancer (MDA MB-231 and rat glioma (C6 cell lines, with IC50 values of 698.0, 648.0 and 1000.0 μg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities.

  14. Mitochondrial respiratory and antioxidative enzyme activities in turkey meat.

    Science.gov (United States)

    Janisch, S; Wicke, M; Krischek, C

    2012-02-01

    Meat quality and (anti)oxidative metabolism of m. pectoralis superficialis (MPS), m. gastrocnemius (MG) and m. iliotibilialis lateralis (MIL) from turkey toms were analysed. After slaughter, pH of MPS and MG decreased and electrical conductivity of the MPS increased. The MG had generally higher pH values. The meat lightness (L) and redness (a) increased in MG and MPS after slaughter. The MPS always had higher L and lower a values. Mitochondrial respiratory activities (MRA) were higher in the MIL than the MPS. The activities of superoxide dismutase (SOD) and glutathione peroxidase, analysed in the MPS, increased and the glutathione reductase activity decreased after slaughter. Meat samples with lower pH24 h p.m. had higher drip loss and L values. The MRA were tendentially lower and the SOD activities higher in these samples. These results indicate a relation between the meat quality, the antioxidative metabolism and mitochondrial respiration.

  15. Purification, structural characterization, and antioxidant activity of antioxidant substance from the red seaweed Gloiopeltis tenax.

    Science.gov (United States)

    Lim, Byung-Lak; Ryu, Il-Hwan

    2009-04-01

    An antioxidant substance (AOS) obtained from an enzymatic extract of the red seaweed Gloiopeltis tenax was purified by DEAE-Sephadex CL-6B and Sephadex G-100 column chromatography. The purification yield of AOS was 14.3%. The AOS predominantly contained fucose, mannose, and galactose but also contained a sulfate group. The structure of AOS was investigated by periodate oxidation, desulfation, Fourier transform-infrared spectroscopy, and nuclear magnetic resonance spectroscopy. AOS was mainly composed of alternating units of beta-D-Glc(1 --> 2)alpha-D-Man(1 --> 4)beta-D-Gal(1 --> 4)alpha-D-Man(1 --> 4)beta-D-Gal alpha-D-Man (1 --> 4) beta-D-Glc (or Xyl)- and branched linkage of alpha-D-Man(1 --> 3) alpha-D-Fuc. In addition, the fucose residues were shown to be 2-O- and 4-O-sulfated and, therefore, were either terminal or 3-linked. The antioxidative activity of AOS was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the beta-carotene-linoleate assay systems and was compared with those of butylated hydroxytoluene and ascorbic acid (AscA). The results showed that AOS exhibited higher antioxidative activity than AscA in the DPPH assay model and in the beta-carotene-linoleate assay system at all of the four concentration levels tested (from 50 to 200 microg/mL). These results suggested that AOS from the red seaweed G. tenax is an efficient novel antioxidant.

  16. Antibacterial and antioxidant activities of Origanum compactum ...

    African Journals Online (AJOL)

    The oil was investigated for its in vitro antibacterial activity against a panel of standard reference strains using well diffusion and broth dilution methods. In solid medium, the oil was found to be remarkably active against all tested strains except Pseudomonas which showed resistance. In liquid medium the Minimum Inhibitory ...

  17. Evaluation of antibacterial, antitumor, antioxidant activities and ...

    African Journals Online (AJOL)

    Results: Generally, yellow loosestrife extracts demonstrated antibacterial activity against Gram-positive bacteria (Staphylococcus aureus, S. epidermidis and Streptococcus pyogenes). Strong antitumor activity of yellow loosestrife was observed via potato disc diffusion bioassay. Nine different phenolics were also determined ...

  18. Antioxidant and antimicrobial activities of callus culture and leaf ...

    African Journals Online (AJOL)

    The callus culture extract (CCE) gave the lowest MIC value of 0.78 mg/ mL for most of the bacteria and fungi and the lowest MBC values of 0.78 mg/ mL and 1.56 mg/ mL against bacteria and fungi, respectively. ... Keywords: Crotalaria retusa; In vitro propagation; Callus culture; Antimicrobial activity; Antioxidant activity ...

  19. Antioxidant and Antiproliferative Activity ofAsparagopsis taxiformis.

    Science.gov (United States)

    Neethu, P V; Suthindhiran, K; Jayasri, M A

    2017-01-01

    Asparagopsis taxiformis ( Rhodophyta ) is a species of red algae belonging to the family Bonnemaisoniaceae . The objective of the present study was to evaluate antioxidant and antiproliferative activity of four fractions (petroleum ether, chloroform, ethyl acetate, and methanol) of A. taxiformis . The red seaweed, A. taxiformis was collected from Mandapam Coastal Region, Gulf of Mannar, Tamil Nadu. Epiphytes present in algal extracts were cleaned and washed with seawater and fresh water. In vitro antioxidant activity was determined by hydrogen peroxide scavenging, ferric reducing antioxidant power, superoxide radical, metal-chelating activity, and phosphomolybdenum reduction assay. Further, the cytotoxic activity was evaluated using brine shrimp lethality assay. This method is rapid, reliable, inexpensive, and convenient as compared to other cytotoxicity assays. Gallic acid, ethylenediaminetetraacetic acid, ascorbic acid, and quercetin were used as reference antioxidant compounds. Reducing power of chloroform extract increased with increasing concentration of the extract. The radical scavenging activity of extracts was in the following order: ascorbic acid > methanol > chloroform > petroleum ether > ethyl acetate. Highest metal-chelating activity was observed in petroleum ether fractions (63%). Reduction of Mo (VI) to Mo (V) increased in methanol extract (27%) at 100 μg/ml. Moreover, all fractions had an inhibitory effect on the formation of hydroxyl radicals. Results showed that ethyl acetate, methanol, and petroleum ether fractions exhibited potent cytotoxic activity with median lethal concentration values of 84.33, 104.4, and 104.4 μg/ml, respectively. Thus, the results showed that red algae possess strong antioxidant and cytotoxic activity that suggests their possible use in the development of pharmaceutical drugs. Various fractions of red algae Asparagopsis taxiformis was evaluated for in vitro antioxidant and antiproliferative studies. All results indicate

  20. Antioxidant activity of alstonia Angustifolia ethanolic leaf extract

    Science.gov (United States)

    Rahim, Nurhidayah Ab; Zakaria, Noorzafiza; Dzulkarnain, Syarifah Masyitah Habib; Azahar, Nazar Mohd Zabadi Mohd; Abdulla, Mahmood Ameen

    2017-10-01

    In current study, the ability of the ethanolic extract of Alstonia angustifolia in scavenging free radicals was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and hydrogen peroxide (H2O2) radical scavenging assay. The results suggested that the ethanolic extract of A. angustifolia leaves has a notable antioxidant activity. In FRAP assay, it showed that the extract have higher total antioxidant activity with FRAP value is 1868.33 µM/g Fe (ii) dry mass ± 0.15 than the control, quercetin with FRAP value is 1336.9 µM/g Fe (II) dry mass ± 0.12 and ascorbic acid with FRAP value is 1720 µM/g Fe (II) dry mass ± 0.02. For DPPH assay, the IC50 value of the extract is 384.77 while the IC50 value of standards of ascorbic acid and quercetin are 18.07 µg/ml and 39.60 µg/ml, respectively. For H2O2 scavenging assay, the IC50 value for the extract was discovered to be 186.77 µg/ml compared to standard ascorbic acid 466.56 µg/ml. Thus, the study suggests that A. angustifolia ethanolic leaf extract has a good origin of natural antioxidants and might be beneficial in impeding the oxidative stress progression thus averting diseases that related to free radicals.

  1. Hydrophilic and lipophilic antioxidant activities of guava fruits.

    Science.gov (United States)

    Thaipong, Kriengsak; Boonprakob, Unaroj; Cisneros-Zevallos, Luis; Byrne, David H

    2005-01-01

    The objectives of this study were to evaluate the hydrophilic antioxidant activity (AOAH) and the lipophilic antioxidant activity (AOAL); and their correlations with vitamin C, and total phenolic and beta-carotene contents in fresh guava fruits of one white flesh clone ('Allahabad Safeda') and three pink flesh clones ('Fan Retief', 'Ruby Supreme,' and an advanced selection). A ferric reducing antioxidant power assay was used to estimate both AOAH and AOAL from methanol and dichloromethane extracts, respectively. The white flesh clone, 'Allahabad Safeda,' showed higher levels of both AOAH [33.3 microM Trolox equivalents (TE)/g fresh weight (FW)] and AOAL (0.25 microM TE/g FW) than the pink flesh clones that ranged from 15.5 to 30.4 and from 0.12 to 0.13 microM TE/g FW for AOAH and AOAL, respectively. The AOAH was positively correlated with vitamin C (r = 0.92, p < 0.01) and total phenolic (r = 0.97, p < 0.01) but was negatively correlated with beta-carotene (r = -0.73, p = 0.03). The AOAL was not correlated with these antioxidants.

  2. Antioxidant activity of oregano essential oil (Origanum vulgare L.

    Directory of Open Access Journals (Sweden)

    Stanojević, Lj.P.

    2016-12-01

    Full Text Available Essential oil obtained from oregano (Origanum vulgare L. by Clevenger-type hydrodistillation and hydromodulus 1:10 m/v during 180 minutes, has been investigated in this work. Qualitative and quantitative composition of the oil was determined by GC-MS and GC-FID spectrometry. Antioxidant activity of the obtained oil was examined spectrophotometrically by DPPH test (after 20, 30, 45 and 60 minutes of incubation and TBA-MDA assay. The yield of essential oil was 4.1 mL/100 g of plant material. Seven components were identified: α-thujene, myrcene, α-terpinene, o-cymene, γ-terpinene, thymol and carvacrol. The major components were thymol (45% and carvacrol (37.4%. Oil incubated for 60 minutes has shown the best antioxidant activity according to DPPH test. The concentrations of essential oil, required for neutralization of 50% of initial DPPH radical concentration (EC50, were 0.761, 0.590, 0.360 and 0.326 mg/mL, after 20, 30, 45 and 60 minutes of incubation, respectively. Lipid peroxidation inhibition of 92.3% was achieved by 1.35 mg/mL essential oil concentration. The results obtained indicate that oregano essential oil is a good source of natural antioxidants with potential application in food and pharmaceutical industries, as a safer alternative to the synthetic antioxidants.

  3. Anti hypoxic and antioxidant activity of Hibiscus esculentus seeds

    Energy Technology Data Exchange (ETDEWEB)

    Ebrahimzadeh, M. A.; Nabavi, S. F.; Nabavi, S. M.; Eslami, B.

    2010-07-01

    The anti hypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Anti hypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The anti hypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC{sub 5}0 for DPPH radical-scavenging activity was 234 {+-} 8.9 {mu}g ml{sup 1}. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml{sup -}1. The extracts showed weak Fe{sup 2}+ chelating ability. IC{sub 5}0 were 150 {+-} 13 {mu}g ml{sup -}1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts. (Author) 40 refs.

  4. Assessment of red onion on antioxidant activity in rat.

    Science.gov (United States)

    Lee, Bora; Jung, Ji-Hye; Kim, Hyun-Sook

    2012-11-01

    Oxidative stress related to the aging process can increase the risk of degenerative disease. Red onions contain antioxidative compounds. This study was designed to investigate the effect of dietary red onion peel and/or flesh on antioxidative activity in rats. Twenty Sprague-Dawley male rats (18 weeks old) were divided into four groups. Each group was raised for 4 weeks on a red onion free control diet (ND), red onion diet containing 5% red onion peel (RP), 5% red onion flesh (RF), or 5% red onion peel+flesh (RPF). The results demonstrated that serum SOD activity was significantly increased in the RP and RPF groups, whereas glutathione peroxidase (GPx) activity was significantly higher in the RF group than in the ND group. Catalase activity and ORAC activity in liver showed upward tendency in the RP, RF, and RPF groups although the differences were not statistically significant. Liver malondialdehyde levels in the RPF group were significantly lower than those in the ND group were. In conclusion, red onion may enhance antioxidant defense mechanism through the induction of plasma SOD and GPx activities and inhibited liver lipid peroxidation. Therefore, red onion may exert important protective effects against oxidative stress related diseases. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

  5. Antioxidant activities of Rambutan (Nephelium lappaceum L peel in vitro

    Directory of Open Access Journals (Sweden)

    Mistriyani,

    2017-09-01

    Full Text Available Rambutan, Nephelium lappaceum L, peel due to consumption of fresh rambutan fruit is taken into account as waste, therefore the exploration of rambutan peel as a natural antioxidant is highly needed. The aim of this study is to investigate the antioxidant activity of rambutan peel from two cultivars (Aceh and Binjai using ABTS radical assay of and ferric reducing activity power (FRAP and to correlate with total phenolics and flavonoids. The powdered rambutan peel is extracted using maceration technique using methanol as extracting solvent. The methanolic extract is added with warm water and fractionated using petroleum ether, chloroform, and ethyl acetate to get corresponding fractions. Rambutan cultivar Binjai revealed the higher ABTS antiradical activity than that of cultivar Aceh. Furthermore, among methanolic extract and its fraction, ethyl acetate fraction exhibited the highest antiradical activity using ABTS radical with IC50 values of 3.10 μg/mL and 0.77 μg/mL for Aceh and Binjai, respectively. The ethyl acetate fraction also revealed the highest FRAP values of 1424.897 ± 28.56 μg/mg fraction sample (Aceh and 968.57± 7.48 μg/mg fraction sample (Binjai. These activities were correlated with phenolics and flavonoid contents. Rambutan peel exhibited strong antioxidant activities, contained high amounts of phenolics and flavonoid and is potential to be developed as a functional food.

  6. Antimicrobial and antioxidant activity of natural honeys of different origin

    Directory of Open Access Journals (Sweden)

    Miartina Fikselová

    2014-10-01

    Full Text Available To examine the antimicrobial and antioxidant activity of 15 natural honeys, honey samples were collected from different locations of Slovakia, Poland and Serbia. For antimicrobial activity determination honey solutions were prepared at three concentrations: 50, 25 and 12.5 % (by mass per volume. The potential antimicrobial activity of  selected samples against four species of bacteria (Escherichia coli CCM 3988, Pseudomonas aeroginosa CCM 1960, Staphylococcus epidermis CCM 4418, Bacillus cereus CCM 2010 and two species of yeasts (Saccharomyces cerevisiae CCM 8191, Candida albicans CCM 8216 was studied using the disc diffusion method. After incubation, the zones of inhibition of the growth of the microorganisms around the disks were measured. The strongest antimicrobial activity was shown at honey samples of 50 % concentration against Escherichia coli, Pseudomonas aeroginosa and Staphylococcus epidermis. Against Saccharomyces cerevisae and Candida albicans very low (at 50 %, 25 % concentration or zero antifugal (at 12.5 % concentration activity was determined. From the results obtained it was shown the variable ability of honey samples to scavenge stable free radical DPPH. TEACDPPH values ranged between 0.1-1.0 mmol.kg-1. As the antioxidative best source buckwheat honey was manifested and the lowest antioxidant activity was shown at acacia honey.

  7. Analogues of the Frog-skin Antimicrobial Peptide Temporin 1Tb Exhibit a Wider Spectrum of Activity and a Stronger Antibiofilm Potential as Compared to the Parental Peptide

    Science.gov (United States)

    Grassi, Lucia; Maisetta, Giuseppantonio; Maccari, Giuseppe; Esin, Semih; Batoni, Giovanna

    2017-04-01

    The frog skin-derived peptide Temporin 1Tb (TB) has gained increasing attention as novel antimicrobial agent for the treatment of antibiotic-resistant and/or biofilm-mediated infections. Nevertheless, such a peptide possesses a preferential spectrum of action against Gram-positive bacteria. In order to improve the therapeutic potential of TB, the present study evaluated the antibacterial and antibiofilm activities of two TB analogues against medically relevant bacterial species. Of the two analogues, TB_KKG6A has been previously described in the literature, while TB_L1FK is a new analogue designed by us through statistical-based computational strategies. Both TB analogues displayed a faster and stronger bactericidal activity than the parental peptide, especially against Gram-negative bacteria in planktonic form. Differently from the parental peptide, TB_KKG6A and TB_L1FK were able to inhibit the formation of Staphylococcus aureus biofilms by more than 50% at 12 μM, while only TB_KKG6A prevented the formation of Pseudomonas aeruginosa biofilms at 24 μM. A marked antibiofilm activity against preformed biofilms of both bacterial species was observed for the two TB analogues when used in combination with EDTA. Analysis of synergism at the cellular level suggested that the antibiofilm activity exerted by the peptide-EDTA combinations against mature biofilms might be due mainly to a disaggregating effect on the extracellular matrix in the case of S. aureus, and to a direct activity on biofilm-embedded cells in the case of P. aeruginosa. Both analogues displayed a low hemolytic effect at the active concentrations and, overall, TB_L1FK resulted less cytotoxic towards mammalian cells. Collectively, the results obtained demonstrated that subtle changes in the primary sequence of TB may provide TB analogues that, used alone or in combination with adjuvant molecules such as EDTA, exhibit promising features against both planktonic and biofilm cells of medically relevant

  8. Antioxidant Activity of Coconut (Cocos nucifera L.) Protein Fractions.

    Science.gov (United States)

    Li, Yan; Zheng, Yajun; Zhang, Yufeng; Xu, Jianguo; Gao, Gang

    2018-03-20

    Coconut cake is an abundant and good potential edible protein source. However, until now it has not been extensively used in the food industry. To promote its usage, the characterization, nutrition value and antioxidant activity of coconut cake protein fractions (albumin, globulin, prolamine, glutelin-1 and glutelin-2) were studied. Results revealed that all the albumin, globulin, glutelin-1 and glutelin-2 fractions showed a high nutrition value. The prolamine, glutelin-1 and glutelin-2 all exhibited good radical scavenging activity and reducing power, and the globulin and prolamine showed high ion chelating ability (89.14-80.38%). Moreover, all the fractions except glutelin-2 could effectively protect DNA against oxidative damage. Several peptides containing five to eight amino acids with antioxidant activity were also identified by LC-MS/MS from the globulin and glutelin-2 fractions. The results demonstrated that the coconut cake protein fractions have potential usages in functional foods.

  9. Orange Peel Extracts: Chemical Characterization, Antioxidant, Antioxidative Burst, and Phytotoxic Activities.

    Science.gov (United States)

    Erukainure, Ochuko L; Ebuehi, Osaretin A T; Iqbal Chaudhary, M; Mesaik, M Ahmed; Shukralla, Ahmed; Muhammad, Aliyu; Zaruwa, Moses Z; Elemo, Gloria N

    2016-01-01

    The search for novel drugs and alternative medicine has led to increased research in medicinal plants. Among such plants is the orange fruit. Its peels have been utilized for long as an active ingredient in most traditional medicines. This study aims at investigating the chemical properties of the hexane and dichloromethane (DCM) extracts of orange peel as well as their biological potentials. Blended peels were extracted with n-hexane and n-dichloromethane, respectively. The resulting extracts were subjected to gas chromatography mass spectrometry (GCMS) characterization. The extracts were also assayed for free radical scavenging ability against 1,1 -diphenyl -2 picrylhydrazyl (DPPH), antioxidative burst via measuring luminol -amplified chemiluminescence response in human blood, and phytotoxicity against lemna minor. GCMS analysis revealed a predominance of fatty acid methyl esters in the hexane extract, while the DCM extract had more ketone metabolites. The DCM extract had significant (p < .05) higher free radical scavenging and antioxidative burst activities compared to the hexane. Both extracts revealed a significantly (p < .05) high phytotoxicity activity. Results from this study indicated that solvent type played a vital a role in the extraction of secondary metabolites, which are responsible for the observed biological activities. The higher activities by the DCM extract can be attributed to its constituents as revealed by GCMS analysis. There is great need to explore the phytotoxicity potentials of both extracts as natural herbicides.

  10. Antioxidant and antibacterial activity of Thai medicinal plant (Capparis micracantha)

    Science.gov (United States)

    Laoprom, Nonglak; Sangprom, Araya; Chaisri, Patcharaporn

    2018-04-01

    This work aims to study the antioxidants capacity, Total phenolic content and antibacterial activity of Thai medicinal plant for the treatment of dermatitis-related inflammations, Capparis micracantha. Crude extract from stem of Thai medicinal plant was extracted with hexane, ethyl acetate, methanol and water. The antioxidant activities (IC50) was evaluated with 1,1-diphenyl-1-princylhydrazyl (DPPH) radical scavenging assay. Total phenolic content (TPC) was determined by using Folin-Ciocalteu method. Bacterial activities was tested with four human pathogenic bacteria; Escherichia coli, Listeria monocytogenes, Staphylococcus aureus and Stapylococcus epidermidis by using agar diffusion assay. Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also determined by broth dilution method. For antioxidant activity, the methanol fraction from stem extract showed the highest activity with an IC50 of 2.4 mg/ml. Water extraction was the high TPC with 10,136.9 mg GAE/g dry weight. Methanol and water extraction showed the remarkable inhibition of bacterial growth was shown against L. monocytogenes and S. aureus. In addition, ethyl acetate, methanol and water fraction from stem extract against S. epidermidis. The present finding suggests that the extract of C. micracantha could be used to discover bioactive natural products that may serve as pharmaceutical products.

  11. Antioxidant and cyclooxygenase activities of fatty acids found in food.

    Science.gov (United States)

    Henry, Geneive E; Momin, Rafikali A; Nair, Muraleedharan G; Dewitt, David L

    2002-04-10

    Several commercially available C-8 to C-24 saturated and unsaturated fatty acids (1-29) were assayed for cyclooxygenase-I (COX-I) and cyclooxygenase-II (COX-II) inhibitory and antioxidant activities. Among the saturated fatty acids tested at 60 microg mL(-1), there was an increase in antioxidant activity with increasing chain length from octanoic acid to myristic acid (C-8-C-14) and a decrease thereafter. All unsaturated fatty acids tested at 60 microg mL(-1) showed good antioxidant activity except for undecylenic acid (12), cis-5-dodecenoic acid (13), and nervonic acid (29). The highest inhibitory activities among the saturated fatty acids tested on cyclooxygenase enzymes COX-I and COX-II were observed for decanoic acid to lauric acid (3-5) at 100 microg mL(-1). Similarly, among the unsaturated fatty acids tested, the highest activities were observed for cis-8,11,14-eicosatrienoic acid (25) and cis-13,16-docosadienoic acid (27) at 100 microg mL(-1).

  12. Chemical composition and antioxidant activity of two strawberry cultivars

    Directory of Open Access Journals (Sweden)

    Đilas Sonja M.

    2011-01-01

    Full Text Available The macro- and micro-chemical composition, as well as antioxidant activity of two strawberry cultivars, Marmolada and Clery, were studied. Results showed a noticeable difference in the sugar, protein and pectin contents. Clery had 6.92% and Marmolada 4.93% of total sugar. Also, protein and pectin contents were higher in the Clery cultivar. No significant difference was observed in acidity, as well as in ash and cellulose content. Marmolada had a higher content of total phenolics and flavonoids (228.04 mg GAE /100 g FW and 136.01 mg RE/100 g FW, respectively . The anthocyanins content in Marmolada (32.0 mg CGE/100 g FW was slightly lower than in Clery (36.0 mg CGE/100 g FW. The antioxidant activity was evaluated spectrophotometrically, using 2,2-diphenyl- 1-picrylhydrazyl (DPPH radical scavenging activity assay. The DPPH free radical scavenging activity, expressed as EC50 value, of Marmolada (0.77 mg/ml was higher than of Clery (0.83 mg/ml. There was a significant positive correlation (R2>0.90 between the concentration of phenolics/flavonoids/anthocyanins and DPPH radical scavenging activity of both strawberry cultivars. These results also showed that the antioxidant value of 100 g FW Marmolada and Clery is equivalent to 237.91 mg and 219.01 mg of vitamin C, respectively.

  13. Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts

    Directory of Open Access Journals (Sweden)

    Chaabane Fadwa

    2012-09-01

    Full Text Available Abstract Background Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological activity in vitro and in vivo. The present study explored antioxidant and antigenotoxic effects of Daphne gnidium leaf extracts. Methods The genotoxic potential of petroleum ether, chloroform, ethyl acetate, methanol and total oligomer flavonoid (TOF enriched extracts from leaves of Daphne gnidium, was assessed using Escherichia coli PQ37. Likewise, the antigenotoxicity of the same extracts was tested using the “SOS chromotest test”. Antioxidant activities were studied using non enzymatic and enzymatic method: NBT/Riboflavine and xantine oxidase. Results None of the different extracts produced a genotoxic effect, except TOF extract at the lowest tested dose. Our results showed that D. gnidium leaf extracts possess an antigenotoxic effect against the nitrofurantoin a mutagen of reference. Ethyl acetate and TOF extracts were the most effective in inhibiting xanthine oxidase activity. While, methanol extract was the most potent superoxide scavenger when tested with the NBT/Riboflavine assay. Conclusions The present study has demonstrated that D. gnidium leaf extract possess antioxidant and antigenotoxic effects. These activities could be ascribed to compounds like polyphenols and flavonoid. Further studies are required to isolate the active molecules.

  14. Antioxidant and antimutagenic activity of Curcuma caesia Roxb. rhizome extracts

    Directory of Open Access Journals (Sweden)

    Heisanam Pushparani Devi

    2015-01-01

    Full Text Available The rhizomes of Curcuma caesia Roxb. (zingiberacea are traditionally used in treatment of various ailments and metabolic disorders like leukoderma, asthma, tumours, piles, bronchitis, etc. in Indian system of medicine. Considering the importance of natural products in modern phytomedicine, the antioxidant and antimutagenic activities of C. caesia Roxb. rhizome extract and its fractions were evaluated. The ethanolic fraction showed highest antioxidant activity by DPPH assay (86.91% comparable to ascorbic acid (94.77% with IC50 value of 418 μg/ml for EECC followed by MECC (441.90 μg/ml > EAECC(561 μg/ml > AECC(591 μg/ml. Based on the antioxidant activity, three of the rhizome extracts were evaluated for their antimutagenic properties against indirect acting mutagen cyclophosphamide (CP using Salmonella typhimurium strains TA98 and TA100. The antimutagenic activity of the extracts against indirect acting mutagen cyclophosphamide in the presence of mammalian metabolic activation system was found to be significant (p < 0.01, p < 0.05. All the extracts showed similar antimutagenicity in dose dependent manner. The total phenolic content as well as reducing ability of the extracts was also determined.

  15. Antioxidative, anticholinesterase and antityrosinase activities of the ...

    African Journals Online (AJOL)

    user

    2012-05-15

    May 15, 2012 ... In addition to undesirable color and flavor, the quinine compounds produced in the browning ... necessary to search and develop novel tyrosinase inhibitors with potent activities and fewer side effects. Dementia is a chronic progressive mental disorder, which adversely affects memory, thinking, compre-.

  16. Bioactive content, hepatoprotective and antioxidant activities of ...

    African Journals Online (AJOL)

    2Department of Pharmacology and Toxicology, Dubai Pharmacy College, Dubai, United Arab Emirate, 3Department of. Pharmaceutical ... biological activities. Methods: Reversed-phase high-performance liquid chromatography (RP-HPLC) was employed for the ..... Yet, clinical application of those plants needs extensive in ...

  17. Fermentation optimization and antioxidant activities of mycelia ...

    African Journals Online (AJOL)

    The mycelia polysaccharides from Morchella esculenta are active ingredients in a number of medicines that play important roles in immunity improvement and tumor growth inhibition. So far, the production of polysaccharides from M. esculenta mycelia has not been commercialized. The aims of this work were to screen and ...

  18. Carotenoids from Phaffia rhodozyma: Antioxidant activity and ...

    African Journals Online (AJOL)

    Aghomotsegin

    2015-06-10

    Jun 10, 2015 ... billion in 2018. They are mostly produced by chemical synthesis. However, the biotechnological production of these pigments by yeasts has been highlighted for possibly using low cost substrates in cultivation, control of metabolites with biological activity, designation of natural substances, the small space ...

  19. Antioxidative, anticholinesterase and antityrosinase activities of the ...

    African Journals Online (AJOL)

    user

    2012-05-15

    May 15, 2012 ... 1Department of Marine Food Science and Technology, Gangeung-Wonju National University, Gangneung 210-702,. Republic of ... The solvent extracts of a red alga Grateloupia lancifolia were used to evaluate biofunctional activities. ...... content, and peroxide value of essential oil and extracts of some.

  20. Antimicrobial, antioxidant and anticancer activities of Strychnos ...

    African Journals Online (AJOL)

    Background: Strychnos lucida R. Br. (Loganiaceae), a well-known indigenous medicine in Timor Leste, has been used for the treatment of ailments such as malaria, diarrhoea, fever, hypertension, cancer, diabetes mellitus and skin infections. Its pharmacological activity has never been reported. The aim of this study was to ...

  1. Antioxidant potentialities and Antiradical Activities of Oxalis ...

    African Journals Online (AJOL)

    SARAH

    2017-08-31

    Aug 31, 2017 ... Eaten raw or added to stew to make it taste, juice taken by sick people to ... Food, medicinal Eaten as raw vegetable, its juice used as medicine to treat, wound, stomach upset, flue in infants and spice to make the stew tasty. 12. Ha. NK. Food and ..... biological activities. For example, Cushine and Lamb.

  2. Hepatoprotective and Antioxidant Activities of Tribulus Terrestris

    NARCIS (Netherlands)

    Harraz, Fathalla M; Ghazy, Nabila M; Hammoda, Hala M; Nafeaa, Abeer A.; Abdallah, Ingy I.

    2015-01-01

    Tribulus terrestris L. has been used in folk medicine throughout history. The present study examined the acute toxicity of the total ethanolic extract of T. Terrestris followed by investigation of the hepatoprotective activity of the total ethanolic extract and different fractions of the aerial

  3. Polyphenols content and antioxidant activity of paprika and pepper spices

    Directory of Open Access Journals (Sweden)

    Soňa Škrovánková

    2017-01-01

    Full Text Available Paprika spices (Capsicium annuum and black pepper spices (Piper nigrum are very popular seasonings for culinary and industrial utilization due to the change of sensory quality (taste, aroma, color of foods and meals with their addition; their health promoting properties; and also, relevant antioxidant activity. Polyphenols are often responsible for the antioxidant capacity of plant products therefore in our study the content of polyphenols (TP and antioxidant activity (TAA were assessed in two common culinary spices - paprika spices (12, ground powder spices and pepper spices (20, unground and ground, black, green, white and colored spices of Czech, Austrian, and Slovak producers. These parameters were determined using spectrometric method, for total polyphenols method with Folin-Ciocaulteu reagent; the antioxidant activity (TAA of aqueous and ethanolic extracts of spices was measured by DPPH method with IC50 evaluation. For paprika the total polyphenol content ranged from 14.67 to 28.78 mg GAE.g-1. However, there is only weak connection between the pungency of the spices and the polyphenolic amount, the hotter samples of paprika spices have slightly higher values of TP than sweet types. Also, more pungent paprika products showed a higher potency in scavenging of DPPH free radical than sweeter ones; and ethanolic extracts had slightly higher TAA values (8.73 to 16.17 mg AAE.g-1 than aqueous spice extracts (4.45 to 16.24 mg AAE.g-1. Phenolic amount for pepper spices was assessed in the range of 12.03 to 22.88 mg GAE.g-1. Generally, paprika spices contained more polyphenols than pepper spices. The values of TAA of pepper spices were in the range from 7.07 to 15.81 mg AAE.g-1 for aqueous extracts and from 8.25 to 15.93 mg AAE.g-1 for ethanolic extracts respectively. The highest TAA values were observed for white ground pepper and unground black pepper spices. Unground black pepper samples had higher TAA than ground black pepper. The extent of

  4. Evaluation of the antioxidant and hepatoprotective activity of Terminalia catappa.

    Science.gov (United States)

    Lin, C C; Chen, Y L; Lin, J M; Ujiie, T

    1997-01-01

    The free radical scavenging and antihepatotoxic activity from Terminalia catappa was studied. Treatment with T. catappa water extracts showed antihepatotoxic activity against CCl4-induced toxicity in the rat liver that was tested. The crude drug also exhibited anti-oxidant effects in FeCl2-Ascorbic acid induced lipid peroxidation in the rat liver homogenate. Moreover, the superoxide radical scavenger effect of T. catappa was demonstrated using electron spin resonance (ESR) and spintrapping technique. The results indicate that T. catappa possesses good antihepatotoxic activity and superoxide radical scavenger activity.

  5. Standardization and in vitro antioxidant activity of jatamansi rhizome

    Directory of Open Access Journals (Sweden)

    Mhaveer Singh

    2015-01-01

    Full Text Available Background: Nardostachys jatamansi Linn. commonly known as jatamansi is a well notorious drug in Indian systems of medicines having various health-related benefits and employed in various herbal formulations due to the presence of high levels of valuable phenolic constituents. The present study was aimed to quality assessment of Jatamansi rhizome by studying macro- and micro-scopic characters along with physicochemical tests, chemo-profiling using thin layer chromatography (TLC, and gas chromatography–mass spectrometry (GC-MS, in vitro antioxidant activity. Materials and Methods: Standardization was carried out as per the pharmacopeial guidelines and contaminant estimation was carried out by analyzing the samples for the determination of heavy metals, pesticides, and aflatoxins. Chemo-profiling was done with TLC by optimizing the mobile phase for different extracts. The GC-MS chemo-profiling was also carried out by using hexane soluble fraction of the hydroalcoholic extract. The drug is well known for a protective role in the human body as an antioxidant, so total phenolic contents and in vitro antioxidant efficacy was also determined by using established methods. Results:The results of quality control and anatomical studies were very much useful for its identification, whereas significant antioxidant efficacy was also observed. The drug was found free of contaminants when analyzed for pesticides and aflatoxins, whereas heavy metals were found under the pharmacopeial limit. Conclusion: The findings of the present research can be utilized for the identification and quality control of the jatamansi rhizome.

  6. Determination of antimicrobial effect, antioxidant activity and ...

    African Journals Online (AJOL)

    In addition, the phenolic contents were determined as follows: the catechin was 20 mg/g, the ferulic acid was 15 mg/g, the p-coumaric acid was 10 mg/g, and the cinnamic acid was 6 mg/g. The results showed that T. boudieri has antimicrobial activity on the gram negative and positive bacteria as well as yeast, and it also has ...

  7. Chemical composition and antioxidant activity of berry fruits

    Directory of Open Access Journals (Sweden)

    Stajčić Slađana M.

    2012-01-01

    Full Text Available The main chemical composition, contents of total phenolic (TPh, total flavonoid (TF, and total monomeric anthocyianin (TMA, as well as the antioxidant activity of two raspberry cultivars (Meeker and Willamette, two blackberry cultivars (Čačanska bestrna and Thornfree and wild bilberry were studied. The raspberry cultivars had the highest total solids among fruits investigated. Bilberry fruits had the highest sugar-to-acid ratio. Blackberry fruits were richer in crude fibers (cellulose in comparison to raspberry and bilberry fruits. The content of pectic substances was highest in the bilberry. Also, bilberry had a highest content of TPh (808.12 mg GAE/100 g FW, TF (716.31 mg RE/100 g FW and TMA (447.83 mg CGE/100 g FW. The antioxidant activity was evaluated spectrophotometrically, using 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging activity assay. The DPPH free radical scavenging activity, expressed as the EC50 value (in mg of fresh weight of berry fruit per ml of the reaction mixture, of bilberry (0.3157 ± 0.0145 mg/ml was the highest. These results also showed that the antioxidant value of 100 g FW bilberry, raspberry - Willamette, raspberry - Meeker, blackberry - Čačanska bestrna and blackberry - Thornfree is equivalent to 576.50 mg, 282.74 mg, 191.58 mg, 222.28 mg and 272.01 mg of vitamin C, respectively. There was a significant positive correlation between the antioxidant activities and content of total phenolics (RTPh 2=0.9627, flavonoids (RTF 2=0.9598 and anthocyanins (RTMA 2=0.9496 in berry fruits. [Projekat Ministarstva nauke Republike Srbije, br. TR 31044

  8. Total phenolics and antioxidant activity of five medicinal plant

    International Nuclear Information System (INIS)

    Sousa, Cleyton Marcos de M.; Silva, Hilris Rocha e; Vieira-Junior, Gerardo Magela; Ayres, Mariane Cruz C.; Costa, Charllyton Luis S. da; Araajo, Delton Servulo; Cavalcante, Luis Carlos D.; Barros, Elcio Daniel S.; Araujo, Paulo Breitner de M.; Brandao, Marcela S.; Chaves, Mariana H.

    2007-01-01

    This paper describes total phenolics content and antioxidant activity in the ethanolic extract of leaves, bark and roots of five medicinal plants: Terminalia brasiliensis Camb., Terminalia fagifolia Mart. and Zucc., Copernicia cerifera (Miller) H.E. Moore, Cenostigma macrophyllum Tul. var. acuminata Teles Freire and Qualea grandiflora Mart. The total phenolics content of the plant extracts, determined by the Folin-Ciocalteu method, varied from 250.0 ±8,2 to 763,63 ±13.03 mg of gallic acid equivalent/g dry EtOH extract. The antioxidant activity of extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay system. Extract of bark from T. brasiliensis, the most active, with an EC 50 value of 27.59 ± 0.82 μg/mL, was comparable to rutin (EC 50 = 27.80 ± 1.38) and gallic acid (EC 50 = 24.27 ± 0.31), used as positive controls. The relationship between total phenolic content and antioxidant activity was positive and significant for T. brasiliensis, C. macrophyllum and C. cerifera. (author)

  9. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities

    Science.gov (United States)

    Hussain, Abdullah Ijaz; Anwar, Farooq; Chatha, Shahzad Ali Shahid; Jabbar, Abdul; Mahboob, Shahid; Nigam, Poonam Singh

    2010-01-01

    The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g-1. The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%), camphor (17.1%), α-pinene (12.3%), limonene (6.23%), camphene (6.00%) and linalool (5.70%). The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP) and one fibroblast cell line (NIH-3T3) using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities. PMID:24031588

  10. Antioxidant and Antimicrobial Activities of Pogostemon cablin (Blanco Benth.

    Directory of Open Access Journals (Sweden)

    Bhanuz Dechayont

    2017-01-01

    Full Text Available Pogostemon cablin (Lamiaceae has been widely used in traditional medicine. In this study, the antioxidant and antimicrobial activities of leaves from P. cablin extracts were investigated. The water extracts had the highest total phenolic content 116.88±0.48 mg gallic acid equivalent/g of dry plant extract. Nevertheless, high levels of total flavonoid content were found in ethanolic extracts 280.12±2.04 mg quercetin equivalent/g of dry plant extract. The highest antioxidant activities were found for the ethanolic extract (IC50=18±0.90, 20±0.24 μg/mL by DPPH and ABTS scavenging assays, respectively. Both extracts showed moderate inhibition of superoxide inhibition (O2∙- and nitric oxide (NO production in concentration-dependent manner. Antibacterial activity was calculated by disk diffusion, minimum inhibitory concentration (MIC, and minimum bacterial concentration (MBC. The ethanolic extract had the greatest activity against methicillin resistant Staphylococcus aureus, methicillin sensitive S. aureus, and Streptococcus pyogenes with zone diameters of 11.67±1.53, 10.33±2.52, and 10.33±1.15 mm, respectively. The corresponding MIC and MBCs were 5, 0.625, and 0.039 mg/mL. P. cablin extracts contain antioxidant and antibacterial properties that should be exploited for possible clinical application.

  11. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities

    Directory of Open Access Journals (Sweden)

    Abdullah Ijaz Hussain

    2010-12-01

    Full Text Available The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g-1. The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%, camphor (17.1%, α-pinene (12.3%, limonene (6.23%, camphene (6.00% and linalool (5.70%. The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP and one fibroblast cell line (NIH-3T3 using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ and minimum inhibitory concentration (MIC of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.

  12. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities.

    Science.gov (United States)

    Hussain, Abdullah Ijaz; Anwar, Farooq; Chatha, Shahzad Ali Shahid; Jabbar, Abdul; Mahboob, Shahid; Nigam, Poonam Singh

    2010-10-01

    The aim of this work was to investigate and compare the antiproliferative, antioxidant and antibacterial activities of Rosmarinus officinalis essential oil, native to Pakistan. The essential oil content from the leaves of R. officinalis was 0.93 g 100g(-1). The GC and GC-MS analysis revealed that the major components determined in R. officinalis essential oil were 1,8-cineol (38.5%), camphor (17.1%), α-pinene (12.3%), limonene (6.23%), camphene (6.00%) and linalool (5.70%). The antiproliferative activity was tested against two cancer (MCF-7 and LNCaP) and one fibroblast cell line (NIH-3T3) using the MTT assay, while, the antioxidant activity was evaluated by the reduction of 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of R. officinalis essential oil, respectively. It is concluded from the results that Rosmarinus officinalis essential oil exhibited antiproliferative, antioxidant and antibacterial activities.

  13. Colour, phenolic content and antioxidant activity of grape juice

    Directory of Open Access Journals (Sweden)

    Vívian Maria Burin

    2010-12-01

    Full Text Available Viticultural practices in the State of Santa Catarina, Brazil, have shown economic growth, with the production of grapes used to produce wines and grape juice. Grapes are rich in phenolic compounds which have drawn attention not only because of their important role in the development of products derived from grapes, but also for their potential beneficial health effects. The objective of this study was to evaluate commercial, organic and homemade grape juices produced in Santa Catarina. Grape juices were analyzed for total phenolic content, colour, and antioxidant activity. The commercial juices had the highest average values for total monomeric anthocyanins and total phenolics. There was a strong positive correlation (R = 0.9566 between the antioxidant activity and total phenolic content for the commercial juice. In addition, the Principle Components Analysis showed a strong positive correlation between the red colour and total monomeric anthocyanins. However, the total monomeric anthocyanis and polymeric anthocyanins showed a negative correlation.

  14. Physicochemical characterization and antioxidant activity of commercial Portuguese honeys.

    Science.gov (United States)

    Aazza, Smail; Lyoussi, Badiaa; Antunes, Dulce; Miguel, Maria Graça

    2013-08-01

    The present study evaluated the physicochemical characteristics and antioxidant activity of 13 commercial honeys from diverse floral origin, produced in Portugal. The values of electrical conductivity of cardoon and pennyroyal honeys were superior to the maximum limits defined by European legislation. Citrus, strawberry tree, and 1 sample of lavender honeys had values of diastase activity below those determined by European legislation. Strawberry tree, pennyroyal, and cardoon honeys had the highest amounts of potassium that coincided with the highest electrical conductivity. Strawberry tree honey was the most effective as antioxidant along with cardoon and heather honeys. This ability was strongly correlated with the amounts of phenols and flavonoids and not with the levels of vitamin C or proline. © 2013 Institute of Food Technologists®

  15. Antimutagenic and antioxidant activities of some bioflavours from wine.

    Science.gov (United States)

    Antonella, Di Sotto; Federico, Durazzi; Grazia, Sarpietro Maria; Gabriela, Mazzanti

    2013-10-01

    Monoterpenes limonene and its metabolic derivatives, α-terpineol and 1,8-cineol, commonly found as aroma wine components, were studied for their antimutagenicity by the bacterial reverse mutation assay on different strains. Substances were also tested for their antioxidant activity, i.e. radical scavenger, chelation, reduction, and lipid peroxidation inhibition. Limonene and its metabolites, α-terpineol and 1,8-cineol, resulted able to inhibit the chemically-induced mutagenesis, although with a different specificity. The antimutagenicity of limonene has been generally retained by its metabolites and sometimes increased. In particular, α-terpineol exhibited the strongest inhibition, moreover it showed to be a remarkable ferrous ions chelating agent. Limonene and 1,8-cineol were devoid of antioxidant activity. Present results are a starting point in evaluating the potential of α-terpineol as a chemopreventive agent and suggest potential functional dietary benefits of wine. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Tannins from Canarium album with potent antioxidant activity*

    Science.gov (United States)

    Zhang, Liang-liang; Lin, Yi-ming

    2008-01-01

    The contents of total phenolics and extractable condensed tannins in the leaves, twigs and stem bark of Canarium album were determined. The structural heterogeneity of condensed tannins from stem bark was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) analyses. The results show the predominance of signals representative of procyanidins and prodelphinidins. In addition, epicatechin and epigallocatechin polymers with galloylated procyanidin or prodelphinidin were also observed. The tannins were screened for their potential antioxidant activities using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) model systems. Tannins extracted from leaves, twigs and stem bark all showed a very good DPPH radical scavenging activity and ferric reducing power. PMID:18500781

  17. Tannins from Canarium album with potent antioxidant activity.

    Science.gov (United States)

    Zhang, Liang-liang; Lin, Yi-ming

    2008-05-01

    The contents of total phenolics and extractable condensed tannins in the leaves, twigs and stem bark of Canarium album were determined. The structural heterogeneity of condensed tannins from stem bark was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) analyses. The results show the predominance of signals representative of procyanidins and prodelphinidins. In addition, epicatechin and epigallocatechin polymers with galloylated procyanidin or prodelphinidin were also observed. The tannins were screened for their potential antioxidant activities using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) model systems. Tannins extracted from leaves, twigs and stem bark all showed a very good DPPH radical scavenging activity and ferric reducing power.

  18. Effects of Salicylic Acid on Carotenoids and Antioxidant Activity of Saffron (Crocus sativus L.

    Directory of Open Access Journals (Sweden)

    Somayeh Tajik

    2015-09-01

    Full Text Available Saffron (Crocus sativus L., the most valuable medicinal food product, belongs to the Iridaceae family which has been widely used as a coloring and flavoring agent. The stigmas contain three major compounds, crocins (carotenoid compound responsible for color, picrocrocin (responsible for taste and safranal (responsible for odor. It has been used for medicinal purposes, as a spice and condiment for food and as a dye since ancient times. Numerous studies have shown crocins as main carotenoids of saffron to be capable of a variety of pharmacological effects, such as protection against cardiovascular diseases, inhibition of cancer cell development. Salicylic acid (SA is a signaling molecule and a hormone-like substance that plays an important role in the plant physiological processes. In order of importance of saffron as valuable product, the aim of this study is to investigate effects of salicylic acid application (0.01, 0.1 and 1 mM on crocin and safranal content and antioxidant activity of stigma. Results showed that SA application at 1 mM were the most effective treatments in increase of crocin content and stronger antioxidant activity, but SA had a negative effect on safranal content and the highest quantity of this compound was observed in control plants.

  19. Purification, antioxidant activity and antiglycation of polysaccharides from Polygonum multiflorum Thunb.

    Science.gov (United States)

    Lv, Lishuang; Cheng, Yunhui; Zheng, Tiesong; Li, Xiaoming; Zhai, Rong

    2014-01-01

    Polysaccharides, one of the most important constituents in Polygonum multiflorum Thunb, a famous Chinese medicinal herb, were isolated by DEAE-52, Sepharose 4B and Sephacryl S-300 column chromatography. Two polysaccharides (PMP-1 and PMP-2) were identified as homogeneous in molecular weight with HPLC. The molecular weights were 4.8 × 10(2) and 6.1 × 10(2) kDa, respectively. Antioxidant activity tests were performed with two polysaccharides at concentrations of 0.1-1.5mg/mL. The results indicated that the inhibitory activity on oxidation and glycation exhibited a dose-dependent response. PMP-2 exhibited a much stronger antioxidant capacity against free radical, lipid oxidation and protein glycation. The IC₅₀ values of PMP-2 were 0.47, 0.6 and 0.93 mg/mL for superoxide anion scavenging, hydroxyl radical scavenging, and hydroxyl peroxide scavenging, respectively. The inhibitory ability of PMP-2 on lipid oxidation was most markedly in rat liver, followed by heart and kidney. Meanwhile, PMP-2 also showed satisfactory suppression of AGEs formation. This suggested that the polysaccharides present in PM can contribute to the biological effects. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Evaluation of the antioxidant activity of rice bran extracts using different antioxidant assays

    Directory of Open Access Journals (Sweden)

    Rehman Bajwa, Jawad -ur-

    2006-09-01

    Full Text Available In the present work the antioxidant activity of different solvent (100% methanol, 80% methanol, 100% acetone, 80% acetone extracts of rice bran was evaluated following different antioxidant assays and using sunflower oil as oxidation substrate. The rice bran extracts were evaluated from the estimate of % inhibition of peroxidation in linoleic acid system, total phenolics content (TPC and loss of β-carotene in a linoleic acid system. Additionally, crude concentrated rice bran extracts were added into the sunflower oil samples and stored under ambient conditions. The extent of oxidative deterioration was followed by the measurement of peroxide-, p-anisidine-, conjugated diene-, and triene- values. The general order of antioxidant efficacy of rice bran extracts as determined by various antioxidant assays was 80% methanolic extract > 100% methanolic  extract > 80% acetone extract > 100% acetone extract. The results of the present comprehensive analysis demonstrate that rice bran extracts of the Super Kernel variety indigenous to Pakistan are a viable source of natural antioxidants and might be exploited for functional foods and nutraceutical applications.Se evalúa la actividad antioxidante diferentes extractos (100% metanol, 80% metanol, 100% acetona and 80% acetona de salvado de arroz -var. Super Kernel- mediante diferentes ensayos y utilizando aceite de girasol como substrato. Los ensayos utilizados fueron la estimación del % de inhibición de la peroxidación en sistemas con ácido linoleico, el contenido total en compuestos fenólicos y la pérdida de β-caroteno en sistemas con ácido linoleico. Adicionalmente, los concentrados de extractos de salvado de arroz se añadieron a aceite de girasol y las muestras se almacenaron a temperatura ambiente. La extensión de la oxidación se evaluó mediante el índice de peróxidos, el índice de p-anisidina, así como la formación de dienos y trienos conjugados. El orden de la eficacia antioxidante

  1. Study on antioxidant activity of Echinacea purpurea L. extracts and ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-05

    Oct 5, 2009 ... This study investigates the antioxidant activity of Echinacea Purpurea L. (EP) extracts and its impact on cell viability. The polysaccharides content of EP was 159.8 ± 12.4 mg/g dry weight (DW), with extracts obtained by applying 55% ethanol at 55°C containing 11.0 ±1.0 mg gallic acid equivalent/g DW of ...

  2. Phytochemistry and antioxidant activity of Lallemantia iberica aerial parts

    Directory of Open Access Journals (Sweden)

    N. Khosravi Dehaghi

    2016-06-01

    Full Text Available Background and objectives:Lallemantia iberica (Lamiaceae is a medicinal plant distributed in different parts of Iran. This research, has evaluated the phytochemical constituents and antioxidant activity of the aerial parts of the plant. Methods: Different chromatographic methods such as column chromatographies using Silica gel (normal and reversed phases, Sephadex LH-20 and HPLC were used for isolation of the compounds from the ethyl acetate and methanol extract of L. iberica aerial parts. The structures of the isolated compounds were elucidated using 1H-NMR, 13C-NMR and EI-MS. Antioxidant activity of the extracts were also evaluated in DPPH and FRAP tests. Results: Two sterols,β-sitosterol acetate (1, β-sitosterol (2, one triterpenoic acid,ursolic acid (3, one polyphenol, rosmarinic acid (4 and six flavonoides,Luteolin-7-O-glucoside (5, 4'-methoxy-luteolin-7-O-glucoside (6, apigenin-7-O-glucoside (7, Luteolin (8,  diosmetin (9, apigenin (10 were isolated and identified from the ethyl acetate and methanol extracts. The antioxidant activity of the ethyl acetate (IC50 189.95±2.8 μg/mL and the methanol extracts (IC50 140±1.2 μg/mL were compared to the standard antioxidant, BHA (IC50 100±1.6 μg/mL in DPPH method. The reducing power of the ethyl acetate (300.28 μmol Eq FeSO4.7H2O/mg DW, the methanol extract (553.14 μmol Eq FeSO4.7H2O/mg DW and BHA (558.36 μmol Eq FeSO4.7H2O/mg of standard were elucidated in FRAP assay. Conclusion: The results introduce L. iberica as a medicinal plant with valuable constituents which are responsible of different pharmacological activities.

  3. Antioxidant activity and acute toxicity of Neoglaziovia variegata ...

    African Journals Online (AJOL)

    The most significant total phenolic content was of 543.50 ± 9.38 mg of gallic acid equivalent/g for ethyl acetate extract (AcOEt), which presented the best antioxidant activity (IC50 5.08 ± 0.20 μg/ml) for DPPH scavenging. The acute toxicity of Nv-EtOH was performed 2.0 g/kg intraperitoneally and 5.0 g/kg orally in mice.

  4. Variation of Antioxidant Activity and Phenolic Content of Some ...

    African Journals Online (AJOL)

    The antioxidant activity in short term air dried Ocimum sanctum plant methanol extract (IC50 = 0.03 ± 0.01 mg/ l) was the highest among the other plant extracts. However, Coriandrum sativum plant ethanol extract produced higher phenolic content (70.24±3.4/66.57±4.1 mg/g) than other plant extracts. It was found that there ...

  5. Phenolic compounds and antioxidant activities in some fruits and ...

    African Journals Online (AJOL)

    The highest values of TEAC were found in garlic (9.6 μmol), okra (3 μmol), spinach (2.2 μmol), tamarind (2.2 μmol) and onion (2.1 μmol). These data revealed that, some local fruits and vegetables from Burkina Faso are potential sources of bioactive compounds. Key words: Antioxidant activity, phenolic compounds, fruits, ...

  6. Soybean Extract Antioxidant Protective Activity Against Copper-Induced Cytotoxicity

    OpenAIRE

    Mansour I. Almansour

    2008-01-01

    The antioxidant activity of soybean crude extract against induced oxidative damage has been achieved through quails Coturnix coturnix treatment with various copper sulphate concentrations alone or with a protective dose of soybean crude extract. Several parameters of oxidative stress together with liver and kidney function tests in serum and liver tissue homogenate were studied. Hematologic indices and liver copper content were determined. Obtained data showed a significant increase in...

  7. Study on antioxidant activity of Echinacea purpurea L. extracts and ...

    African Journals Online (AJOL)

    This study investigates the antioxidant activity of Echinacea Purpurea L. (EP) extracts and its impact on cell viability. The polysaccharides content of EP was 159.8 ± 12.4 mg/g dry weight (DW), with extracts obtained by applying 55% ethanol at 55°C containing 11.0 ±1.0 mg gallic acid equivalent/g DW of total phenolic ...

  8. Antioxidant and antimicrobial activities of Bauhinia racemosa L. stem bark

    Directory of Open Access Journals (Sweden)

    Kumar R.S.

    2005-01-01

    Full Text Available The present study was carried out to evaluate the antioxidant and antimicrobial activities of a methanol extract of Bauhinia racemosa (MEBR (Caesalpiniaceae stem bark in various systems. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH radical, superoxide anion radical, nitric oxide radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the extract. The antioxidant activity of the methanol extract increased in a concentration-dependent manner. About 50, 100, 250, and 500 µg MEBR inhibited the peroxidation of a linoleic acid emulsion by 62.43, 67.21, 71.04, and 76.83%, respectively. Similarly, the effect of MEBR on reducing power increased in a concentration-dependent manner. In DPPH radical scavenging assays the IC50 value of the extract was 152.29 µg/ml. MEBR inhibited the nitric oxide radicals generated from sodium nitroprusside with an IC50 of 78.34 µg/ml, as opposed to 20.4 µg/ml for curcumin. Moreover, MEBR scavenged the superoxide generated by the PMS/NADH-NBT system. MEBR also inhibited the hydroxyl radical generated by Fenton's reaction, with an IC50 value of more than 1000 µg/ml, as compared to 5 µg/ml for catechin. The amounts of total phenolic compounds were also determined and 64.7 µg pyrocatechol phenol equivalents were detected in MEBR (1 mg. The antimicrobial activities of MEBR were determined by disc diffusion with five Gram-positive, four Gram-negative and four fungal species. MEBR showed broad-spectrum antimicrobial activity against all tested microorganisms. The results obtained in the present study indicate that MEBR can be a potential source of natural antioxidant and antimicrobial agents.

  9. Improved antioxidant activity in transgenic Perilla frutescens plants via overexpression of the γ-tocopherol methyltransferase (γ-tmt) gene.

    Science.gov (United States)

    Ghimire, Bimal Kumar; Seong, Eun Soo; Lee, Chan Ok; Lee, Jae Geun; Yu, Chang Yeon; Kim, Seung Hyun; Chung, Ill Min

    2015-09-01

    The main goal of this study was to generate transgenic Perilla frutescens with enhanced antioxidant properties by overexpressing the γ-tocopherol methyltransferase (γ-tmt) gene. In this study, the antioxidant activity of methanolic crude extracts of transgenic and non-transgenic control plants was investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Free radical scavenging activity was evaluated using α-tocopherol and butylated hydroxyl toluene as standard antioxidants. In general, the ethyl acetate fraction of transgenic P. frutescens showed stronger DPPH radical scavenging activity than the ethyl acetate fraction from non-transgenic control plants (IC50 2.00 ± 0.10 and 5.53 ± 0.40 μg ∙ ml(-1), respectively). High-performance liquid chromatography analysis of phenolic acids in leaf extracts confirmed increased levels of 16 individual phenolic compounds in two transgenic lines (pf47-5 and pf47-8) compared with control plants. Changes in the phenolic compound profile and α-tocopherol content were correlated with the antioxidant properties of transgenic plants, indicating that the introduction of transgene γ-tmt influenced the metabolism of phenolic compounds and subsequently produced biochemical changes in the transformants. There were no significant differences in photosynthetic rate in the transgenic plants as compared to the non-transgenic control plants, suggesting that the alteration of phenolic compounds and tocopherol composition had little impact on photosynthesis.

  10. Modification of the cellular antioxidant activity (CAA) assay to study phenolic antioxidants in a Caco-2 cell line.

    Science.gov (United States)

    Kellett, Mary E; Greenspan, Phillip; Pegg, Ronald B

    2018-04-01

    In vitro assays are widely used to analyze the antioxidant potential of compounds, but they cannot accurately predict antioxidant behavior in living systems. Cell-based assays, like the cellular antioxidant activity (CAA) assay, are gaining importance as they provide a biological perspective. When the CAA assay was employed to study phenolic antioxidants using hepatocarcinoma (HepG2) cells, quercetin showed antioxidant activity in HepG2 cells; 25 and 250μM quercetin reduced fluorescence by 17.1±0.9% and 58.6±2.4%, respectively. (+)-Catechin, a phenolic antioxidant present in many foods, bestowed virtually no CAA in HepG2 cells. When Caco-2 cells were employed, more robust antioxidant activity was observed; 50μM (+)-catechin and quercetin reduced fluorescence by 54.1±1.4% and 63.6±0.9%, respectively. Based on these results, likely due to differences in active membrane transport between the cell types, the Caco-2-based CAA assay appears to be a more appropriate method for the study of certain dietary phenolics. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Antioxidant and Hepatoprotective Activities of Flavonoids from Bauhinia hookeri

    Directory of Open Access Journals (Sweden)

    Eman Al-Sayed

    2016-05-01

    Full Text Available In a previous study, the total ethanol extract of Bauhinia hookeri showed a significant hepatoprotective effect in CCl 4-induced toxicity model in mice. However, the active components responsible for the activity were not identified. Therefore, this study was undertaken to determine if the activity of B. hookeri extract is due to its flavonoid content. The hepatoprotective activity of B. hookeri flavonoids was determined by measuring the activities of alanine aminotransferase (ALT and aspartate aminotransferase (AST in the culture medium of HepG2 cells challenged with CCl 4. The lipid peroxidation and antioxidant parameters, superoxide dismutase (SOD and glutathione (GSH were estimated in the cell lysates. The isolated flavonoids were identified by mass, UV and NMR spectral data. This study revealed that B. hookeri flavonoid fraction and its pure compounds ( kaempferol 3-O-β- D-glucoside, quercetin 3-O-β- D-glucoside and c atechin 3-O-α- L-rhamnoside possess a promising hepatoprotective activity as evidenced from the normalized levels of ALT and AST. This was attributed partly to their potent antioxidant activity as demonstrated by the increased GSH levels, SOD activity and reduced lipid peroxidation. The whole flavonoid fraction showed the highest cytoprotective activity and was more effective than silymarin. This study highlights a promising natural hepatoprotective remedy derived from B. hookeri.

  12. Chemical profiling and antioxidant activity of Bolivian propolis.

    Science.gov (United States)

    Nina, Nélida; Quispe, Cristina; Jiménez-Aspee, Felipe; Theoduloz, Cristina; Giménez, Alberto; Schmeda-Hirschmann, Guillermo

    2016-04-01

    Propolis is a relevant research subject worldwide. However, there is no information so far on Bolivian propolis. Ten propolis samples were collected from regions with high biodiversity in the main honey production places in Bolivia and were analyzed for their total phenolics (TP), flavonoids (TF) and antioxidant activity. The chemical profiles of the samples were assessed by TLC, HPLC-DAD, HPLC-DAD-MS/MS(n) and NMR analysis. TP, TF, TLC and NMR analysis showed significant chemical differences between the samples. Isolation of the main constituents by chromatography and identification by HPLC-DAD-MS/MS(n) achieved more than 35 constituents. According to their profiles, the Bolivian propolis can be classified into phenolic-rich and triterpene-rich samples. Propolis from the valleys (Cochabamba, Chuquisaca and Tarija) contained mainly prenylated phenylpropanoids, while samples from La Paz and Santa Cruz contained cycloartane and pentacyclic triterpenes. Phenolic-rich samples presented moderate to strong antioxidant activity while the triterpene-rich propolis were weakly active. High chemical diversity and differential antioxidant effects were found in Bolivian propolis. Our results provide additional evidence on the chemical composition and bioactivity of South American propolis. © 2015 Society of Chemical Industry.

  13. Antioxidant, Antigenotoxic, Antimicrobial Activities and Phytochemical Analysis of Dianthus carmelitarum

    Directory of Open Access Journals (Sweden)

    Rezzan Aliyazicioglu

    2017-05-01

    Full Text Available In this study, we investigated the phytochemical composition, antioxidant, antimicrobial, and antigenotoxic properties of the aqueous extract of Dianthus carmelitarum for the first time. The phenolic and volatile compounds, antioxidant, antimicrobial and antigenotoxic activities of the extract were determined by HPLC and SPME-GC-FID/MS, spectrophotometric, agar well diffusion methods and comet assay, respectively. The polyphenolic content and ferric reducing power values of the extract were found 12.6 ± 0.27 mg gallic acid and 238 ± 2.89 μM trolox equivalents per g sample, respectively. Syringaldehyde and chlorogenic acid were detected as major phenolic compounds, while terpenes were determined as major volatile compound. Dianthus carmelitarum extract especially exhibited moderate antimicrobial activity against Mycobacterium smegmatis. Extract reduced H 2O 2-induced DNA damage in a concentration dependent manner in fibroblast cells compared to positive control (only 20 μM H 2O 2 treatment. Dianthus carmelitarum can be considered in the food, cosmetic, and drug industries due to its antioxidant, antimicrobial, and antigenotoxic activities.

  14. Antioxidant activity of medicinal plant extracts in cookies.

    Science.gov (United States)

    Mišan, Aleksandra; Mimica-Dukić, Neda; Sakač, Marijana; Mandić, Anamarija; Sedej, Ivana; Simurina, Olivera; Tumbas, Vesna

    2011-01-01

    The antioxidant activity of ethanolic extracts of parsley, buckthorn, mint, caraway, and their mixture "Vitalplant" was evaluated, and the potential of "Vitalplant" mixture extract to retard the process of lipid oxidation in cookies was tested. The antioxidant activity was estimated by 2 direct (ESR) and 4 indirect (spectrophotometric) tests and correlated with the total phenolic and flavonoid content. The potential of "Vitalplant" mixture extract to retard the process of lipid oxidation in cookies was measured by thiobarbituric acid (TBA)-reactive-substances assay (TBARS) and DPPH˙ (1,1-diphenyl-2-picryl-hydrazyl) test. Significantly different (P buckthorn > "Vitalplant" > parsley > caraway. Total flavonoid content varied from 0.510% (parsley) to 2.05% ("Vitalplant"). A statistically significant correlation was found between IC(50) values on DPPH˙ and total flavonoid content of the samples (r=- 0.94, P cookies in dose-dependent manner. Supplementation of cookies with a mixture of Petroselini fructus, Frangulae cortex, Mentha piperitae folium, Carvi fructus can retard the process of lipid oxidation and elevate antioxidant activity of the final product. © 2011 Institute of Food Technologists®

  15. Antioxidant Activities of Polyphenols Extracted from Perilla frutescens Varieties

    Directory of Open Access Journals (Sweden)

    Linghua Meng

    2008-12-01

    Full Text Available Various cultivars of Perilla frutescens (L. (var. crispa and var. frutescens Britt. were harvested in China and Japan. They were easily differentiated on the basis of their foliage color, that varied from red to green. Water extracts of dried plants were investigated for their antioxidant activity (AA and their polyphenolic compounds compared. Among them, cinnamic acid derivatives (coumaroyl tartaric acid, caffeic acid and rosmarinic acid, flavonoids (apigenin 7-O-caffeoylglucoside, scutellarein 7-O-diglucuronide, luteolin 7-O-diglucuronide, apigenin 7-O-diglucuronide, luteolin 7-O-glucuronide, and scutellarein 7-O-glucuronide and anthocyanins (mainly cis-shisonin, shisonin, malonylshisonin and cyanidin 3-O-(E-caffeoylglucoside-5-O-malonylglucoside were quantified. AA assays are based on the inhibition of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH. The DPPH radical scavenging activity was calculated as Trolox ® [(±-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid] equivalent antioxidant capacity (TEAC. The mean amount of total phenolics of the water extracts (4-29 µmol/100 mL and the TEAC value calculated (23-167 µmol TE/100 mL confirmed the high antioxidant activity of these leaf water extracts. These results were highly correlated within some o-dihydroxylated polyphenolic compounds and AA.

  16. Mineral components and anti-oxidant activities of tropical seaweeds

    Science.gov (United States)

    Takeshi, Suzuki; Yumiko, Yoshie-Stark; Joko, Santoso

    2005-07-01

    Seaweeds are known to hold substances of high nutritional value; they are the richest resources of minerals important to the biochemical reactions in the human body. Seaweeds also hold non-nutrient compounds like dietary fiber and polyphenols. However, there is not enough information on the mineral compounds of tropical seaweeds. Also we are interested in the antioxidant activities of seaweeds, especially those in the tropical area. In this study, Indonesian green, brown and red algae were used as experimental materials with their mineral components analyzed by using an atomic absorption spectrophotometer. The catechins and flavonoids of these seaweeds were extracted with methanol and analyzed by high performance liquid chromatography (HPLC); the antioxidant activities of these seaweeds were evaluated in a fish oil emulsion system. The mineral components of tropical seaweeds are dominated by calcium, potassium and sodium, as well as small amounts of copper, iron and zinc. A green alga usually contains epigallocatechin, gallocatechin, epigallocatechin gallate and catechin. However, catechin and its isomers are not found in some green and red algae. In the presence of a ferrous ion catalyst, all the methanol extracts from the seaweeds show significantly lower peroxide values of the emulsion than the control, and that of a green alga shows the strongest antioxidant activity. The highest chelation on ferrous ions is also found in the extract of this alga, which is significantly different from the other methanol extracts in both 3 and 24 h incubations.

  17. Evaluating Antiproliferative and Antioxidant Activity of Marrubium crassidens

    Directory of Open Access Journals (Sweden)

    Sanaz Hamedeyazdan

    2014-10-01

    Full Text Available Purpose: Naturally occurring substances as novel drugs in cancer therapy, at all times, represent a challenge to science since medicinal plants are proving to be brilliant sources of new chemopreventive agents. Methods: In the present study, methanol extract from aerial parts of Marrubium crassidens was assessed for its antiproliferative activity in the breast cancer cell line MCF-7 through MTT bioassay using cell viability and cytotoxicity indices. The antioxidant property of M. crassidens extract together with its phenolic and flavonoids content were evaluated, as well. Results: According to data obtained in the study, M. crassidens exhibited antiproliferative activity with a gradual rise in cytotoxicty effect setting out on 240μg/mL concentration of the extract. Moreover, the RC50 value for antioxidant activity of the extract was determined as 40μg/mL and values for the total phenolic and flavonoids were calculated as 512.64mg gallic acid equivalent and 212.73mg quercetin equivalent per 100g of dry plant material. Conclusion: Generally, the observed antiproliferative and antioxidant properties of M. crassidens could be certified to the high amounts of phenolic and flavonoid content detected in the extract.

  18. Evaluating Antiproliferative and Antioxidant Activity of Marrubium crassidens.

    Science.gov (United States)

    Hamedeyazdan, Sanaz; Sharifi, Simin; Nazemiyeh, Hossein; Fathiazad, Fatemeh

    2014-10-01

    Naturally occurring substances as novel drugs in cancer therapy, at all times, represent a challenge to science since medicinal plants are proving to be brilliant sources of new chemopreventive agents. In the present study, methanol extract from aerial parts of Marrubium crassidens was assessed for its antiproliferative activity in the breast cancer cell line MCF-7 through MTT bioassay using cell viability and cytotoxicity indices. The antioxidant property of M. crassidens extract together with its phenolic and flavonoids content were evaluated, as well. According to data obtained in the study, M. crassidens exhibited antiproliferative activity with a gradual rise in cytotoxicty effect setting out on 240µg/mL concentration of the extract. Moreover, the RC50 value for antioxidant activity of the extract was determined as 40µg/mL and values for the total phenolic and flavonoids were calculated as 512.64mg gallic acid equivalent and 212.73mg quercetin equivalent per 100g of dry plant material. Generally, the observed antiproliferative and antioxidant properties of M. crassidens could be certified to the high amounts of phenolic and flavonoid content detected in the extract.

  19. Antioxidant Activity and Bioactive Components of Oxalidaceae Fruit Extracts

    International Nuclear Information System (INIS)

    Asna, N.A.; Noriham, A.

    2014-01-01

    The study was conducted to evaluate the Antioxidant Activity (AA), Total Flavonoid Content (TFC), and Total Phenolic Content (TPC) of Oxalidaceae fruit extracts. Two types of Averrhoa carambola L. and two types of bilimbi fruits were used in this study. Bilimbi fruits selected were Averrhoa bilimbi L. and Averrhoa bilimbi cv. while Averrhoa carambola L. selected were Averrhoa carambola L. (honey type) and another type of Averrhoa carambola L. which is known as tart type. The maturity stage for both Averrhoa carambola L. fruits (honey and tart type) were selected at commercial maturity stages which were stage 3 and stage 4. Antioxidant estimation of oxalidaceae fruit extracts were evaluated by using Total Flavonoid Content (TFC) and Total Phenolic Content (TPC), while antioxidant activity were evaluated using scavenging of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), β-carotene Bleaching (BCB) and Ferric Reducing Antioxidant Power (FRAP) assays. Phytochemical screening of alkaloids, flavonoids, terpenoids, steroids, tannins and saponins were also performed on all samples. Averrhoa carambola L. indicated positive results for all phytochemical screening conducted and similar results were observed from bilimbi fruits, except for alkaloids. Averrhoa carambola L. (tart type) of stage 4 possessed higher TPC and TFC followed by Averrhoa carambola L. (honey type) of stage 4. Averrhoa carambola L. (tart type) of stage 4 also exhibited high scavenging effect (74 %) as determined by DPPH assay with the value of EC 50 72.36 mg/ ml while Averrhoa carambola L. (honey type) of stage 3 showed significantly lower percentage of scavenging effect. The results also demonstrated that FRAP values of Averrhoa carambola L. (honey type) of stage 4 and Averrhoa carambola L. (tart type) of stage 4 were higher than other samples (5.1023 mmol TE/ g and 5.0759 mmol TE/ g) and similar trends were observed for β-carotene Bleaching (BCB) assay, where Averrhoa carambola L. (honey type) of stage 4

  20. Invitro Antioxidant Activities on Leaf Extracts of Syzygium Malaccense (L.) Merr and Perry

    OpenAIRE

    Savitha, Rabeque C; Padmavathy, S; Sundhararajan, A

    2011-01-01

    Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of various diseases. The objective of the study was to determine the quantitative phytochemicals and invitro antioxidant activity of fresh leaves of Syzygium malaccense. The result showed that the methanolic extract exhibited strong antioxidant activity and contains a higher amount of phenolics and flavonoids when compared to aqueous extract.

  1. Invitro antioxidant activities on leaf extracts of syzygium malaccense (L.) merr and perry.

    Science.gov (United States)

    Savitha, Rabeque C; Padmavathy, S; Sundhararajan, A

    2011-04-01

    Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of various diseases. The objective of the study was to determine the quantitative phytochemicals and invitro antioxidant activity of fresh leaves of Syzygium malaccense. The result showed that the methanolic extract exhibited strong antioxidant activity and contains a higher amount of phenolics and flavonoids when compared to aqueous extract.

  2. In vitro antioxidant activities of the fractions of Coccinia grandis l. leaf ...

    African Journals Online (AJOL)

    The present study was aimed at investigating the antioxidant activities of the various fractions of the hydromethanolic extract of the leaves of Coccinia grandis L. Voigt. (Cucurbitaceae). The antioxidant activities of the fractions have been evaluated by using nine in vitro assays and were compared to standard antioxidants ...

  3. Chemical composition and antioxidant activities of Broussonetia papyrifera fruits.

    Directory of Open Access Journals (Sweden)

    Jie Sun

    Full Text Available Fruits of Broussonetia papyrifera from South China were analyzed for their total chemical composition, and antioxidant activities in ethanol and aqueous extracts. In the fruit of this plant, the crude protein, crude fat and carbohydrates was 7.08%, 3.72% and 64.73% of dry weight, respectively. The crude protein, crude fat and carbohydrates were 15.71%, 20.51% and 36.09% of dry weight, respectively. Fatty acid and amino acid composition of the fruit were analyzed. Unsaturated fatty acid concentration was 70.6% of the total fatty acids. The percentage of the essential amino acids (EAAs was 40.60% of the total amino acids. Furthermore, B. papyrifera fruit are rich in many mineral elements and vitamins. Total phenolic content was assessed using the Folin-Ciocalteau assay, whereas antioxidant activities were assessed by measuring the ability of the two extracts to scavenge DPPH radicals, inhibit peroxidation, and chelate ferric ions. Their reducing power was also assessed. Results indicated that the aqueous extract of B. papyrifera was a more potent reducing agent and radical-scavenger than the ethanol extract. GC-MS analysis of the ethanol extract showed the presence of some acid-containing compounds. The changes in total phenolic content and antioxidant capacity in B. papyrifera from four different regions grown under normal conditions were assessed. The antioxidant activity of different extracts was positively associated with their total phenolic content. These results suggest that the fruit of B. papyrifera could be used in dietary supplement preparations, or as a food additive, for nutritional gain, or to prevent oxidation in food products.

  4. Antioxidant activity of extracts obtained from residues of different oilseeds.

    Science.gov (United States)

    Matthäus, B

    2002-06-05

    Residues of the oil-extracting process of oilseeds contain bioactive substances such as phenolic compounds, which could be used as natural antioxidants for the protection of fats and oils against oxidative deterioration. Thus, the extraction of such constituents from residual material can be considered to contribute to the added value of these residues, which could justify their isolation. In the present work the fat-free residues of eight different oilseeds whose oils are usable for nutritional applications, and also as renewable resources, were extracted with 70% methanol, 70% acetone, water, and ethyl acetate/water, respectively. The resulting extracts were investigated regarding their content of total phenolic compounds by the Folin-Ciocalteau assay, sinapine, flavanoids, and the UV-absorption spectra. Further, the antioxidant activity of the extracts was characterized by the DPPH method, the beta-carotene-linoleic acid assay, and ESR investigations. The fat-free residues of the different oilseeds contained considerable amounts of extractable substances. The yields decreased with decreasing polarity of the solvent in the order water, 70% methanol, 70% acetone, and ethyl acetate. The ratio of total phenolic compounds to the extractable compounds ranged from 3 to 19%. There was no significant correlation between the amount of total extractable compounds and the total phenolic compounds (p < 0.001). All extracts showed remarkable antioxidant activities determined with the different methods. The effects depended strongly on the solvent used for the extraction as well as on the extracted residue. A correlation between the methods used for the characterization of the antioxidant activity and the composition of the residues could not be shown.

  5. Antioxidant and antimicrobial activities of native Rosa sp. from British Columbia, Canada.

    Science.gov (United States)

    Yi, Ocksook; Jovel, Eduardo M; Towers, G H Neil; Wahbe, Tanya R; Cho, Dongwuk

    2007-05-01

    Indigenous traditional knowledge and western science have revealed the potential for significant nutritional and therapeutic benefits among natural antioxidants. We investigated antioxidant and antimicrobial activity of rose hip extracts (Rosa nutkana, Rosa pisocarpa and Rosa woodsii) from wild British Columbia populations using liposome oxidation and disc diffusion assays. All extracts exhibited strong antioxidant activity. R. nutkana pericarp extracts contained high phenolic concentrations and showed greater antioxidant and antimicrobial activity than seed extracts. R. woodsii seed extracts had a higher phenolic concentration and greater antioxidant activity than pericarp extracts. Antioxidant activity was correlated with antimicrobial activity, and both extracts showed antimicrobial activity against yeast and Gram-positive bacteria. Our study is the first to demonstrate the antioxidant and antimicrobial properties of wild British Columbia roses. The effectiveness of selected species compared with standards demonstrates the significance of this natural resource to the continued health of human populations, and the need for conservation practices.

  6. Identification of Major Phenolic Compounds from Nephelium lappaceum L. and Their Antioxidant Activities

    OpenAIRE

    Thitilertdecha, Nont; Teerawutgulrag, Aphiwat; Kilburn, Jeremy D.; Rakariyatham, Nuansri

    2010-01-01

    Nephelium lappaceum is a tropical fruit whose peel possesses antioxidant properties. Experiments on the isolation and identification of the active constituents were conducted, and on their antioxidant activity using a lipid peroxidation inhibition assay. The methanolic extract of N. lappaceum peels exhibited strong antioxidant properties. Sephadex LH-20 chromatography was utilized in the isolation of each constituent and the antioxidant properties of each was studied. The isolated compounds w...

  7. Antioxidant Activity of Spices and Their Impact on Human Health: A Review

    OpenAIRE

    Yashin, Alexander; Yashin, Yakov; Xia, Xiaoyan; Nemzer, Boris

    2017-01-01

    Antioxidants are substances that prevent oxidation of other compounds or neutralize free radicals. Spices and herbs are rich sources of antioxidants. They have been used in food and beverages to enhance flavor, aroma and color. Due to their excellent antioxidant activity, spices and herbs have also been used to treat some diseases. In this review article, the chemical composition and antioxidant activity of spices and culinary herbs are presented. The content of flavonoids and total polypheno...

  8. Antioxidant Activities and Polyphenolic Contents of Three Selected Micromeria Species from Croatia

    OpenAIRE

    Vladimir-Knežević, Sanda; Blažeković, Biljana; Štefan, Maja Bival; Alegro, Antun; Kőszegi, Tamás; Petrik, József

    2011-01-01

    Antioxidant activities of three selected Micromeria species growing in Croatia (M. croatica, M. juliana and M. thymifolia) were evaluated using five different antioxidant assays, in comparison with plant polyphenolic constituents and reference antioxidants. All studied ethanolic extracts exhibited considerable activity to scavenge DPPH and hydroxyl free radicals, reducing power, iron chelating ability and total antioxidant capacity in the order: M. croatica > M. juliana > M. thy...

  9. Antimicrobial and Antioxidant Activities of Plants from Northeast of Mexico

    Directory of Open Access Journals (Sweden)

    Ricardo Salazar-Aranda

    2011-01-01

    Full Text Available Traditional medicine has a key role in health care worldwide. Obtaining scientific information about the efficacy and safety of the plants from our region is one of the goals of our research group. In this report, 17 plants were selected and collected in different localities from northeast Mexico. The dried plants were separated into leaves, flowers, fruit, stems, roots and bark. Each part was extracted with methanol, and 39 crude extracts were prepared. The extracts were tested for their antimicrobial activity using three Gram-negative bacterial strains (Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii, three Gram-positive bacterial strains (Enterococcus faecalis and two Staphylococcus aureus strains, and seven clinically isolated yeasts (Candida albicans, C. krusei, C. tropicalis, C. parapsilosis and C. glabrata; their antioxidant activity was tested using a DPPH free radical assay. No activity against Gram-negative bacteria was observed with any extract up to the maximum concentration tested, 1000 μg ml−1. We report here for the first time activity of Ceanothus coeruleus against S. aureus (flowers, minimal inhibitory concentration (MIC 125 μg ml−1, C. glabrata (MICs 31.25 μg ml−1 and C. parapsilosis (MICs between 31.25 and 125 μg ml−1; Chrysanctinia mexicana against C. glabrata (MICs 31.25 μg ml−1; Colubrina greggii against E. faecalis (MICs 250 μg ml−1 and Cordia boissieri against C. glabrata (MIC 125 μg ml−1. Furthermore, this is the first report about antioxidant activity of extracts from Ceanothus coeruleus, Chrysanctinia mexicana, Colubrina greggii and Cyperus alternifolius. Some correlation could exist between antioxidant activity and antiyeast activity against yeasts in the species Ceanothus coeruleus, Schinus molle, Colubrina greggii and Cordia boissieri.

  10. Polysaccharides in Sipunculus nudus: Extraction condition optimization and antioxidant activities

    Science.gov (United States)

    Zhang, Qin; Dong, Lanfang; Tong, Tong; Wang, Qingchao; Xu, Mingzhu

    2017-02-01

    Marine organisms constitute unlimited resource of bioactive substances due to their high biodiversity and represent a valuable source of new compounds. This study optimized the alkali-extraction conditions and antioxidant activities of soluble polysaccharides from the body wall of Sipunculus nudus. The effects of solid-liquid ratio, extraction duration, extraction temperature, and alkali concentration on the yield of S. nudus polysaccharides (SNP) were examined, according to which the optimal combination of extraction parameters was obtained by an orthogonal test. The relative influencing importance of different extraction parameters on the yield of SNP followed the order as solid-liquid ratio > extraction temperature > alkali concentration > extraction duration. The highest extraction yield, 1.98%, was achieved under an optimal extraction condition: temperature 60°C, solid-liquid ratio 1:6 g mL-1, duration 5 h, and alkali (NaOH) mass fraction 6%. The in vitro antioxidant activities examination showed that extracted SNP under this optimized condition had strong power in reducing certain hydroxyl and superoxide radical scavenging abilities. The promising results showed that extracted SNP could be a potent natural antioxidant.

  11. Phenolic Compounds and Antioxidant Activity of Phalaenopsis Orchid Hybrids

    Directory of Open Access Journals (Sweden)

    Truong Ngoc Minh

    2016-09-01

    Full Text Available Phalaenopsis spp. is the most commercially and economically important orchid, but their plant parts are often left unused, which has caused environmental problems. To date, reports on phytochemical analyses were most available on endangered and medicinal orchids. The present study was conducted to determine the total phenolics, total flavonoids, and antioxidant activity of ethanol extracts prepared from leaves and roots of six commercial hybrid Phalaenopsis spp. Leaf extracts of “Chian Xen Queen” contained the highest total phenolics with a value of 11.52 ± 0.43 mg gallic acid equivalent per g dry weight and the highest total flavonoids (4.98 ± 0.27 mg rutin equivalent per g dry weight. The antioxidant activity of root extracts evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay and β-carotene bleaching method was higher than those of the leaf extracts. Eleven phenolic compounds were identified, namely, protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, syringic acid, vanillin, ferulic acid, sinapic acid, p-coumaric acid, benzoic acid, and ellagic acid. Ferulic, p-coumaric and sinapic acids were concentrated largely in the roots. The results suggested that the root extracts from hybrid Phalaenopsis spp. could be a potential source of natural antioxidants. This study also helps to reduce the amount of this orchid waste in industrial production, as its roots can be exploited for pharmaceutical purposes.

  12. Activity-Guided Identification of in Vitro Antioxidants in Beer.

    Science.gov (United States)

    Spreng, Stefan; Hofmann, Thomas

    2018-01-24

    In order to locate the key antioxidants contributing to oxidative stability of beer, activity-guided fractionation in combination with the oxygen radical absorbance capacity (ORAC) assay, hydrogen peroxide scavenging (HPS) assay, and linoleic acid (LA) assay was applied to a pilsner-type beer. LC-MS and 1D/2D NMR experiments led to the identification of a total of 31 antioxidants, among which 3-methoxy-4-hydroxyphenyl-β-d-glucopyranoside (tachioside), 4-(2-formylpyrrol-1-yl)butyric acid, 4-[2-formyl-5-(hydroxymethyl)pyrrol-1-yl]butyric acid, n-multifidol-3-O-β-d-glucoside, quercetin-3-O-(6″-malonyl)-glucoside, 4-feruloylquinic acid, syringaresinol, saponarin, and hordatines A-C have been isolated from beer for the first time. On a molar comparison, the hordatines A-C, saponarin, and quercetin-3-O-β-d-(6″-malonyl)glucoside were evaluated with the highest antioxidant activities of all identified beer constituents, reaching values of 10-17.5 (ORAC), 2.0-4.1 (HPS), and 1.1-6.1 μmol TE/μmol (LA) for hordatines A-C.

  13. Evaluation of antioxidant activity of Melittis melissophyllum L. extracts

    Directory of Open Access Journals (Sweden)

    Grujić Slavica M.

    2014-01-01

    Full Text Available The antioxidant activities of methanol and ethanol (10%, 30%, 50% and 96% extracts of the aerial parts of Melittis melissophyllum L. were determined by DPP H, ABTS and FRAP assays. The content of flavonoids and phenols was also investigated. The total phenolic content in the extracts was determined using Folin-Ciocalteu assay; their amounts ranged between 63.5 and 111.7 mg GAeqv/g, while the concentrations of flavonoids were from 7.33 to 56.00 mg Queqv/g. IC50 values of the DPP H scavenging effect were from 0.109 to 0.664 mg/mL. The DPP H scavenging effect of the extracts was determined and the obtained IC50 values were from 0.109 to 0.664 mg/mL of solution. The values of ABTS radical activity were from 0.45 to 0.89 mg ascorbic acid/g. The FRAP value was within the range 0.160-0.382 mmol Fe/mg. The obtained values were analyzed by means of multivariate analysis, employing a hierarchical cluster analysis and between-groups linkage. The presented results confirmed that M. melissophyllum possesses good antioxidant properties and may serve as a promising source of natural antioxidants. [Projekat Ministarstva nauke Republike Srbije, br. 173029

  14. Antinociceptive and antioxidant activity of Zanthoxylum budrunga wall (Rutaceae) seeds.

    Science.gov (United States)

    Islam, Md Khirul; Biswas, Nripendra Nath; Saha, Sanjib; Hossain, Hemayet; Jahan, Ismet Ara; Khan, Tanzir Ahmed; Awang, Khalijah; Shilpi, Jamil A

    2014-01-01

    Different parts of the medicinal plant Zanthoxylum budrunga Wall enjoy a variety of uses in ethnobotanical practice in Bangladesh. In the present study, a number of phytochemical and pharmacological investigations were done on the ethanol extract of Z. budrunga seeds (ZBSE) to evaluate its antinociceptive and antioxidant potential. ZBSE was also subjected to HPLC analysis to detect the presence of some common antioxidants. In acetic acid induced writhing test in mice, ZBSE showed 65.28 and 74.30% inhibition of writhing at the doses of 250 and 500 mg/kg and the results were statistically significant (P Zanthoxylum budrunga seed in traditional medicine for pain management. Constituents including caffeic acid and other phenolics might have some role in the observed activity.

  15. Chemical constituents and antioxidant activity of Byrsonima gardneriana (Malpighiaceae)

    International Nuclear Information System (INIS)

    Rolim, Thaisa Leite; Wanderley, Flavia Talita de Sousa; Cunha, Emidio Vasconcelos Leitao da; Tavares, Josean Fechine; Oliveira, Adriana Maria Fernandes de; Assis, Temilce Simoes

    2013-01-01

    The phytochemical investigation of Byrsonima gardneriana led to the isolation of five triterpenes and one flavonoid: D:B-Friedoolean-5-en-3-one (1), friedoolean-14-en-3-one (2), friedelan-3-one (3), lup-20(29)-en-3-ol (4), 3β-hydroxiolean-12-ene (5) and 3,3',4',5,7-pentahydroxyflavan (6). Their structures were assigned based on spectroscopic analyses, including two-dimensional NMR techniques and comparison with published spectral data. Antioxidant activities of ethanol extract and phases were measured using the 1,2-diphenyl- 2-picryl-hydrazyl (DPPH) free radical scavenging assay, evaluation of total phenolic content and trolox equivalent antioxidant capacity (TEAC). (author)

  16. Strawberry and human health: effects beyond antioxidant activity.

    Science.gov (United States)

    Giampieri, Francesca; Alvarez-Suarez, José M; Battino, Maurizio

    2014-05-07

    The usefulness of a diet rich in vegetables and fruits on human health has been widely recognized: a high intake of antioxidant and bioactive compounds may in fact play a crucial role in the prevention of several diseases, such as cancer, cardiovascular, neurodegenerative, and other chronic pathologies. The strawberry (Fragaria × ananassa Duch.) possesses a remarkable nutritional composition in terms of micronutrients, such as minerals, vitamin C, and folates, and non-nutrient elements, such as phenolic compounds, that are essential for human health. Although strawberry phenolics are known mainly for their anti-inflammatory and antioxidant actions, recent studies have demonstrated that their biological activities also spread to other pathways involved in cellular metabolism and cellular survival. This paper has the main objective of reviewing current information about the potential mechanisms involved in the effects elicited by strawberry polyphenols on human health, devoting special attention to the latest findings.

  17. Antioxidative activity of caffeic acid: Mechanistic DFT study

    Directory of Open Access Journals (Sweden)

    Redžepović Izudin

    2017-01-01

    Full Text Available This paper reports the results of comprehensive mechanistic investigations of the hydrogen atom transfer (HAT, radical adduct formation (RAF, single electron transfer – proton transfer (SET-PT, and sequential proton loss electron transfer (SPLET mechanisms of caffeic acid (CA. The goals of the work were achieved by simulating the reactions of CA with hydroxyl radical in benzene and water solutions. It was found that SET-PT is not a favourable antioxidative mechanism of CA. On the other hand, HAT and RAF are competitive, because HAT pathways yield thermodynamically more stable radical products, and RAF pathways require smaller activation barriers. In polar basic environment SPLET is a probable antioxidative mechanism of CA, with exceptionally large rate.

  18. Phytotoxic and antioxidant activity of seven native fruits of Brazil

    Directory of Open Access Journals (Sweden)

    Marize Terezinha Lopes Pereira Peres

    2013-12-01

    Full Text Available We investigated antioxidant capacity, phenolic content and phytotoxic effects in seven species of fruit native to Brazil: Acrocomia aculeata, Hymenaea stigonocarpa, Tamarindus indica, Byrsonima intermedia, Psidium firmum, Myrciaria cauliflora and Ficus obtusifolia. Phytotoxic effects were assessed from crude ethanol extracts and semipurified fractions. In four of the seven fruits, total phenolic content, as determined by Folin-Ciocalteu assay, was higher in the peel than in the pulp. We found that total phenolic content correlated significantly with antioxidant capacity. High-performance liquid chromatography with diode array detection revealed the presence of the flavonoids quercetin and rutin in some fruits. We also evaluated the allelopathic effects of the crude ethanol extract and semipurified fractions. In most of the essays, the ethyl acetate fractions showed the highest level of activity.

  19. Antioxidant Activity and Phenolic Content of Apple Cider

    Directory of Open Access Journals (Sweden)

    Nilgün Havva Budak

    2015-03-01

    Full Text Available Fruit and vegetables are an important component of a healthy diet and the main antioxidant suppliers in the human diet. Consumption of foods derived from fruits and vegetables is also essential; fruit juices, ciders, wines, and vinegars also contain significant amounts of polyphenolic compounds. The aim of the study was to determine the effect maceration of antioxidant activity and phenolic content of apple cider. Red delicious apples were used to produce natural apple cider with and without inclusion of maceration. Samples were taken from fresh red apple juice, macerated samples and apple cider. Apple cider (maceration was applied (CAM had the highest total phenolic content, chlorogenic acid, ORAC and TEAC levels. Chlorogenic acid was the dominant phenolic substance in apple juice and cider samples and chlorogenic acid was increased with maceration process.

  20. Composition, antioxidant and antimicrobial activity of the essential oil of Achillea collina Becker growing wild in Western Romania

    Directory of Open Access Journals (Sweden)

    Jianu Călin

    2015-01-01

    Full Text Available The investigation of the chemical composition, antioxidant and antimicrobial properties of the essential oil of Achillea collina Becker growing wild in western Romania was the aim of this study. The chemical composition of the essential oil was evaluated by GC-MS. The major compounds identified were chamazulene (38.89%, germacrene D (12.90%, beta-caryophyllene (11.52% and beta-pinene (10.66%. The antimicrobial activity was assessed by the diffusimetric method against seven common food-related bacteria. No effects were observed against Clostridium perfringens and Streptococcus pyogenes. The antioxidant activity was evaluated using the DPPH test, the essential oil (IC50 = 25.03 ± 0.12 μg/ml demonstrated a stronger scavenging effect than BHA and lower than that of ascorbic acid and propyl gallate. The results reveal strong antimicrobial and antioxidant properties of the essential oil tested and contribute to future research to find new sources of natural antiseptics and antioxidants: a viable and safe alternative to reduce the use of synthetic additives.

  1. Antioxidants in Raspberry: On-line analysis links antioxidant activity to a diversity of individual metabolites

    NARCIS (Netherlands)

    Beekwilder, M.J.; Jonker, H.H.; Hall, R.D.; Meer, van der I.M.; Vos, de C.H.

    2005-01-01

    The presence of antioxidant compounds can be considered as a quality parameter for edible fruit. In this paper, we studied the antioxidant compounds in raspberry (Rubus idaeus) fruits by high-performance liquid chromatography (HPLC) coupled to an on-line postcolumn antioxidant detection system. Both

  2. Antioxidant activity of olive pulp and olive oil phenolic compounds of the arbequina cultivar.

    Science.gov (United States)

    Morelló, José-Ramón; Vuorela, Satu; Romero, Maria-Paz; Motilva, Maria-José; Heinonen, Marina

    2005-03-23

    The aim of this study was to characterize antioxidant activities of phenolic compounds that appear in olive pulp and olive oils using both radical scavenging and antioxidant activity tests. Antiradical and antioxidant activities of olive pulp and olive oil phenolic compounds were due mainly to the presence of a 3,4-dihydroxy moiety linked to an aromatic ring, and the effect depended on the polarity of the phenolic compound. Glucosides and more complex phenolics exhibited higher antioxidant activities toward oxidation of liposomes, whereas in bulk lipids aglycons were more potent antioxidants with the exception of oleuropein. Lignans acted as antioxidants only in liposomes, which could partly be due to their chelating activity, because liposome oxidation was initiated by cupric acetate. The antioxidant activity of virgin olive oil is principally due to the dialdehydic form of elenolic acid linked to hydroxytyrosol (3,4-DHPEA-EDA), a secoiridoid derivative (peak RT 36, structure unidentified), and luteolin.

  3. HPTLC Analysis, Antioxidant and Antigout Activity of Indian Plants.

    Science.gov (United States)

    Nile, Shivraj Hariram; Park, Se Won

    2014-01-01

    The HPTLC analysis, antioxidant, and antigout activity of Asparagus racemosus, Withania somnifera, Vitex negundo, Plumbago zeylanica, Butea monosperma and Tephrosia purpurea extracts were investigated. The chemical fingerprinting were carried out by high performance thin layer chromatography (HPTLC), antioxidant activity by ABTS, DPPH, FRAP radical scavenging assays, and antiogout activity by cow milk xanthine oxidase. The HPTLC fingerprint qualitatively revealed predominant amount of flavonoids. The TEAC values ranged from 45.80 to 140 µM trolox/100 g dry weight for ABTS, from 85 to 430 µM trolox/ 100 g dw DPPH, and 185 to 560 µM trolox/100 g dw for FRAP respectively. Plants used in this study was found to inhibit the toxicity, as seen from the decreased LPO and increased GSH, SOD and CAT levels. The total phenolic and flavonoid content ranged from 10.21 to 28.17 and 5.80 to 10.1 mg of gallic acid equivalents (GAE)/100 gdw respectively. The plant extracts demonstrated significant xanthine oxidase inhibitory activity at 100 g/mL and revealed an inhibition greater than 50 % and IC50 values below the standard. This effect was almost similar to the activity of allopurinol (Standard drug) against xanthine oxidase (90.2 ± 0.4 %). These plant root extract will be subjected for further extensive studies to isolate and identify their active constituents which are useful for against inflammation and gout.

  4. Substituent Effects on the Stability and Antioxidant Activity of Spirodiazaselenuranes

    Directory of Open Access Journals (Sweden)

    Devappa S. Lamani

    2015-07-01

    Full Text Available Spirodiazaselenuranes are structurally interesting compounds and the stability of these compounds depends highly on the nature of the substituents attached to the nitrogen atoms. Aromatic substituents are known to play important roles in stabilizing the Se-N bonds in spiro compounds. In this study, several spirodiazaselenuranes are synthesized by introducing benzylic and aliphatic substituents to understand their effect on the stability of the Se-N bonds and the antioxidant activity. Replacement of phenyl substituent by benzyl/alkyl groups significantly reduces the stability of the spirodiazaselenuranes and slows down the oxidative cyclization process. The selenium centre in the spiro compounds undergoes further oxidation to produce the corresponding selenurane oxides, which are stable at room temperature. Comparison of the glutathione peroxidase (GPx mimetic activity of the compounds showed that the diaryl selenides having heterocyclic rings are significantly more active due to the facile oxidation of the selenium centre. However, the activity is reduced significantly for compounds having aliphatic substituents. In addition to GPx activity, the compounds also inhibit peroxynitrite-mediated nitration and oxidation reaction of protein and small molecules, respectively. The experimental observations suggest that the antioxidant activity is increased considerably upon substitution of the aromatic group with the benzylic/aliphatic substituents on the nitrogen atoms.

  5. Extraction, antioxidant and antibacterial activities of Broussonetia papyrifera fruits polysaccharides.

    Science.gov (United States)

    Han, Qiaohong; Wu, Zili; Huang, Bo; Sun, Liangqi; Ding, Chunbang; Yuan, Shu; Zhang, Zhongwei; Chen, Yanger; Hu, Chao; Zhou, Lijun; Liu, Jing; Huang, Yan; Liao, Jinqiu; Yuan, Ming

    2016-11-01

    Polysaccharides were extracted from Broussonetia papyrifera ((L.) L'Herit. ex Vent.) fruits (BPP), and response surface methodology was used to maximize extraction yield. The optimum extraction conditions were: ratio of water to solid, 30mL/g; extraction duration, 50min; extraction power, 180W; and extraction temperature, 60°C. Under these conditions, the yield of BPP was 8.61%. Then, BPP was purified, and three purified fractions (designated BPP-1, BPP-2 and BPP-3) were obtained for further physicochemical properties, antioxidant activity and antibacterial activity analysis. These fractions were mainly composed of glucose, mannose and arabinose residue, meanwhile, BPP-3 had a significantly higher rhamnose and uronic acid content than BPP-1 and BPP-2. And BPP-3 showed the best hydroxyl radial scavenging activity, ferric reducing activity power (FRAP), antihemolytic activity and antibacterial activity. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. Antioxidant, Anticholinesterase and Antibacterial Activities of Jurinea consanguinea DC

    Directory of Open Access Journals (Sweden)

    Hülya Öztürk

    2011-01-01

    Full Text Available The aim of this study was to investigate in vitro antioxidant, anticholinesterase and antibacterial activities of the petroleum ether, chloroform and methanol extracts obtained from the aerial parts of Jurinea consanguinea DC. (Asteraceae. Total phenolic and flavonoid contents of these crude extracts were determined as pyrocatechol and quercetin equivalents, respectively. The methanol extract which possessed almost the same effect with the chloroform extract in b -carotene-linoleic acid system exhibited higher free radical scavenging activity than a standard compound, BHT, at 100 and 200 m g/mL concentrations. The petroleum ether extract showed the highest acetylcholinesterase inhibitory activity among the tested extracts. The methanol extract exhibited higher butyrylcholinesterase inhibitory activity than galantamine at all concentrations (94% inhibition at 200 m g/mL. The antibacterial activity of the extracts was determined by the disc diffusion method. The chloroform extract showed moderate activity against B. subtilis, P. aeruginosa and S. aureus.

  7. Antibacterial, Antifungal and antioxidant activities of some medicinal plants.

    Science.gov (United States)

    Wazir, Asma; Mehjabeen, -; Jahan, Noor; Sherwani, Sikander Khan; Ahmad, Mansoor

    2014-11-01

    The purpose of this study was to evaluate the antibacterial, antifungal and antioxidant activities of medicinal plants. The antibacterial activity of methanolic extracts of three medicinal plants (Swertia chirata, Terminalia bellerica and Zanthoxylum armatum) were tested against Gentamicin (standard drug) on eleven gram positive and seventeen gram negative bacteria by agar well method. It was revealed that seven-gram negative and six gram positive bacterial species were inhibited by these plant extracts. Minimum inhibitory concentrations (MIC) of the extracts were determined by broth micro-dilution method. The significant MIC value of Swertia chirata was 20mg/ml against Serratia marcesens, Zanthoxylum armatum was 10 mg/ml against Aeromonas hydrophila and Terminali bellerica was 20mg/ml against Acinetobacter baumanii as well as Serratia marcesens. Antifungal screening was done for methanolic extracts of these plants by agar well method with the 6 saprophytic, 5 dermatophytic and 6 yeasts. In this case Griseofulvin was used as a standard. All saprophytes and dermatophytes were showed resistance by these plants extracts except Microsporum canis, which was inhibited by Z. armatum and S. chirata extracts. The significant MIC value of Zanthoxylum armatum was 10mg/ml against Microsporum canis and Swertia chirata was 10mg/ml against Candida tropicalis. The anti-oxidant study was performed by DPPH free radical scavenging assay using ascorbic acid as a reference standard. Significant antioxidant activities were observed by Swertia chirata and Zanthoxylum armatum at concentration 200μg/ml was 70% DPPH scavenging activity (EC50=937.5μg/ml) while Terminalia bellerica showed 55.6% DPPH scavenging activity (EC50=100μg/ml). This study has shown that these plants could provide potent antibacterial compounds and may possible preventive agents in ROS related ailments.

  8. Ultrasonic degradation of sweet potato pectin and its antioxidant activity.

    Science.gov (United States)

    Ogutu, Fredrick Onyango; Mu, Tai-Hua

    2017-09-01

    The effect of ultrasound factors (time, power, and duty cycle) on sweet potato pectin molecular weight, neutral sugar composition, pectin structure, and antioxidant activity was investigated. Sweet potato pectin dispersions (0.0025, 0.005 and 0.01g/mL) in deionized water were sonolyzed for 5, 10 and 20min to assess effect of sonication time and pectin concentration on sonolysis. For further experiments 0.0025g/mL was sonicated under varying ultrasonic power and duty cycle levels, subsequently the molecular weight, galacturonic acid content, degree of methoxylation and antioxidant activity of sonicated pectin products were investigated. Results showed that ultrasound treatment reduced pectin molecular weight, while polydispersity did not show clear trend which characterized random pectin scission, increasing duty cycle from 20% to 80% resulted in approximately threefold reduction in pectin molecular weight, increased sonication power from 100W to 400W led to significant increase in galacturonic acid content from 72.0±1.2% in native pectin to between 85.0±3.2% and 92.0±2.7%, the degree of methoxylation significantly reduced from 12.0±3.0% to between 5.25% and 6.28%, sonication led to increase in galactose and decrease in rhamnose consistent with debranching of pectin. Moreover, sonication lead to increased antioxidant capacity, both 200W and 400W sonicated pectin having higher ORAC and FRAP values, with highest pectin concentration 4mg/mL in ORAC and 0.8mg/ml in FRAP giving substantially high antioxidant activity than native and 100W treated pectin. The ORAC value of 400W sonicated pectin increased five hold above the native pectin, while it's FRAP value was almost three fold higher than native pectin. However, ultrasound did not alter pectin primary structure as showed by FTIR and HPAEC results. The results indicated that ultrasound offers effective and green process for pectin transformation and creation of antioxidant potent pectin products. Copyright © 2016

  9. Highly antioxidant carotene-lipid nanocarriers: synthesis and antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Lacatusu, Ioana; Badea, Nicoleta, E-mail: nicoleta.badea@gmail.com; Ovidiu, Oprea [University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Science (Romania); Bojin, Dionezie [Faculty of Engineering and Materials Science (Romania); Meghea, Aurelia [University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Science (Romania)

    2012-06-15

    The objective of this study was to explore the potential of two natural oils (squalene-Sq and grape seed oil-GSO) to prepare biocompatible antioxidant nanostructured lipid carriers-NLCs as a safety and protective formulation for sensitive {beta}-carotene. For this purpose different oil-in-water nanoemulsions stabilized by a combination of alkylpolyoxy ethylene sorbitans, lecithin and a block copolymer, were prepared using a melt high-shear homogenization process. The physico-chemical characteristics of the carotene-loaded NLCs were firstly investigated in detail. The smaller lipid nanoparticles have been obtained by using Tween 20 as main non-ionic surfactant, with average diameters of about 85 nm for GSO and 89 nm for Sq, with a polydispersity index <0.19. The developed carotene-NLCs presented an excellent physical stability with almost all zeta potential values ranging between -29 Division-Sign -40 mV. The differential scanning calorimetry analysis showed that the {beta}-carotene incorporation has led to a perturbation of solid lipid matrix with a less ordered arrangement. By UV-Vis spectroscopy it was evidenced that after encapsulation {beta}-carotene adopts a supramolecular structure demonstrated by appearance of a shoulder at 530 nm related to a {beta}-carotene triplet-triplet absorption. The carotene-NLCs have been also evaluated in terms of in vitro antioxidant properties. The presence of Sq and GSO produced a significant effect on the antioxidant capacity of developed NLCs. The samples prepared with GSO and Tween 80 as main surfactant showed the highest antioxidant activity (AA %) against free oxygen radicals, exhibiting an enhancement of 35 % for loaded NLCs, as comparing to pure carotene. In addition to these properties, the ability of NLCs to manifest antibacterial activity was tested against Escherichiacoli bacteria. The antibacterial analysis shown that loaded-NLCs develop an effective inhibition zone against bacteria growth and it was dependent in a

  10. Study of antioxidant activity and free radical scavenging power of ...

    African Journals Online (AJOL)

    In recent years, the use of synthetic antioxidants and the degradation products derived from them is limited, because they are toxic and carcinogenic. So, studying on the use of natural antioxidants to replace the synthetic antioxidants is very important. Natural antioxidants are healthier and have more benefits and fewer ...

  11. Syntheses of Resveratrol Analogues and Evaluation of Their Antioxidant Activity

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Mi Jeong; Jung, Se Hoon; Moon, Insu; Jun Jonggab; Lee, Jeong Tae [Hallym Univ., Chuncheon (Korea, Republic of)

    2014-05-15

    Free radicals such as superoxide anion radicals (O{sub 2}·{sup -}), hydroxyl radicals (·OH) and non-free radical species such as hydrogen peroxide (H{sub 2}O{sub 2}) and singlet oxygen ({sup 1}O{sub 2}) are considered as ROS. These ROS not only oxidize membrane lipids but damage nucleic acids, proteins and carbohydrates leading to mutations. If ROS are not scavenged by antioxidants, they could be involved in ageing and various diseases related to oxidative stress. Resveratrol is a natural phytoalexin found in the skin of grapes, red wines, and peanuts. It has three hydroxyl groups at the trans-stilbene structure, in which resorcinol and phenol are bridged by a trans double bond. The recent extensive studies on the resveratrol and its derivatives revealed that they have antioxidant, antimutagenic, antiinflammatory, antidiabetic, cardiovascular protective, and anticancer properties. It has been believed that the majority of the biological functions of resveratrol has been attributed to its antioxidant activity.

  12. Chocolate consumption, fecal water antioxidant activity, and hydroxyl radical production.

    Science.gov (United States)

    Record, Ian R; McInerney, Jennifer K; Noakes, Manny; Bird, Anthony R

    2003-01-01

    As part of a larger study into the effects of polyphenols derived from chocolate on bowel health we have compared the effects of consumption of chocolate containing either 200 mg of flavanols and related procyanidins or a similar chocolate containing less than 10 mg of polyphenols on fecal free radical production and antioxidant activity in 18 volunteers. In a double-blind crossover trail volunteers consumed chocolate for two 4-wk periods separated by a 4-wk washout period. During the time the volunteers consumed the chocolate they also consumed a low-polyphenol diet. Free radical production in the fecal water was lowered from 122 +/- 10 micromol/l/h to 94 +/- 9 micromol/l/h (P = 0.009) when the high procyanidin chocolate diet was consumed and from 117 +/- 14 micromol/l/h to 86 +/- 12 micromol/l/h when the low procyanidin chocolate was consumed (P = 0.014). Fecal water antioxidant capacity measured by either the Trolox equivalent antioxidant capacity or ferric reducing ability of plasma procedure was not significantly affected. Consumption of either chocolate reduced the production of free radicals in fecal water. This suggests that some component of the chocolate other than the flavanols and related procyanidins may have been effective.

  13. Foeniculum vulgare essential oils: chemical composition, antioxidant and antimicrobial activities.

    Science.gov (United States)

    Miguel, Maria Graça; Cruz, Cláudia; Faleiro, Leonor; Simões, Mariana T F; Figueiredo, Ana Cristina; Barroso, José G; Pedro, Luis G

    2010-02-01

    The essential oils from Foeniculum vulgare commercial aerial parts and fruits were isolated by hydrodistillation, with different distillation times (30 min, 1 h, 2 h and 3 h), and analyzed by GC and GC-MS. The antioxidant ability was estimated using four distinct methods. Antibacterial activity was determined by the agar diffusion method. Remarkable differences, and worrying from the quality and safety point of view, were detected in the essential oils. trans-Anethole (31-36%), alpha-pinene (14-20%) and limonene (11-13%) were the main components of the essentials oil isolated from F. vulgare dried aerial parts, whereas methyl chavicol (= estragole) (79-88%) was dominant in the fruit oils. With the DPPH method the plant oils showed better antioxidant activity than the fruits oils. With the TBARS method and at higher concentrations, fennel essential oils showed a pro-oxidant activity. None of the oils showed a hydroxyl radical scavenging capacity > 50%, but they showed an ability to inhibit 5-lipoxygenase. The essential oils showed a very low antimicrobial activity. In general, the essential oils isolated during 2 h were as effective, from the biological activity point of view, as those isolated during 3 h.

  14. Sempervivum davisii: phytochemical composition, antioxidant and lipase-inhibitory activities.

    Science.gov (United States)

    Uzun, Yusuf; Dalar, Abdullah; Konczak, Izabela

    2017-12-01

    Sempervivum davisii Muirhead (Crassulaceae) is a traditional medicinal herb from Eastern Anatolia. To date the composition of phytochemicals and physiological properties of this herb were not subjected to any research. This study identifies compounds in S. davisii hydrophilic extracts and evaluates their potential biological properties. Ethanol-based lyophilized extracts were obtained from aerial parts of plant (10 g of ground dry plant material in 200 mL of acidified aqueous ethanol, shaken for 2 h at 22 °C with supernatant collected and freeze-dried under vacuum). Phytochemical composition was investigated by liquid chromatography mass spectrometry (LC-MS/MS, phenolics) and gas chromatography mass spectrometry (GC-MS, volatiles). Phenolic compounds were quantified by high-performance liquid chromatography (HPLC) and the Folin-Ciocalteu assay. Subsequently, antioxidant capacity [ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC) assays] and enzyme inhibitory properties (isolated porcine pancreatic lipase) of the extracts were determined. Polyphenolic compounds were the main constituents of lyophilized extracts, among which kaempferol glycosides and quercetin hexoside dominated. The extracts exhibited potent antioxidant (FRAP values of 1925.2-5973.3 μM Fe 2+ /g DW; ORAC values of 1858.5-4208.7 μM Trolox Eq./g DW) and moderate lipase inhibitory (IC 50 : 11.6-2.96 mg/mL) activities. Volatile compounds (nonanal, dehydroxylinalool oxide isomers, 2-decenal, 2-undecenal, 2,6-di-tetr-butylphenol) were also found. Phenolic compounds with the dominating kaempferol and quercetin derivatives are the sources of potent antioxidant properties of S. davisii hydrophilic extracts. The extracts exhibit moderate inhibitory properties towards isolated pancreatic lipase.

  15. Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae

    OpenAIRE

    F. Xavier Malcata; Pedro Moradas-Ferreira; Paula Tamagnini; A. C. Silva Ferreira; Maria S. Gião; Rui Seabra; A. Catarina Guedes

    2013-01-01

    A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation) ABTS method. For complem...

  16. Phytochemical Screening, Antimicrobial and Antioxidant Activities of Ficus natalensis

    International Nuclear Information System (INIS)

    Ajaib, M.

    2016-01-01

    Phytochemical screening, antimicrobial and antioxidant potential of the bark and leaves extracts of Ficus natalensis were carried out by using various techniques. Phytochemical analysis showed the presence of terpenoids, alkaloids, flavonoids, tannins, saponins, cardiac glycosides and reducing sugars in different extracts of Ficus natalensis. The antibacterial potential against S. aureus was reported as most promising amongst all. The petroleum ether extract of leaves with a zone of inhibition 50 ± 0.51 mm and bark extracts with a zone of inhibition 55.7 ± 1.15 mm inhibited S. aureus. The chloroform leaves extract also showed an inhibition zone of 50 ± 2 mm against S. aureus. The antifungal potential of methanol bark extract at 43.7 ±1.527 mm and petroleum ether extracts of bark with zones of inhibition 37 ± 0.577 mm against A. niger showed most prominent activity. By using different assays, the extracts were screened for the antioxidant potential. The estimation of antioxidant activity by metal chelating activity revealed that water extract of leaves was most active with a value of 74.673 ± 0.302 percentage bound iron. The chloroform extract of bark showed highest flavonoid content (1005.53 ± 0.503 mg/mL of quercetin), whereas chloroform extract of leaves exhibited maximum phenolic content (21.626 ± 0.545 mg/g of GAE). In ABTS assay, water extract of leaves showed maximum TEAC value (7.713 ± 0.7 mM of trolox equivalent). The highest free radical scavenging DPPH percentage was observed with distilled water extract of bark (91.92 ± 0.08 percent). (author)

  17. Characterization of antioxidant activity of extract from Artemisia vulgaris.

    Science.gov (United States)

    Temraz, Abeer; El-Tantawy, Walid H

    2008-10-01

    Recent investigations have shown that the antioxidant properties of plants could be correlated with oxidative stress defense and different human diseases. In this respect flavonoids and other polyphenolic compounds have gained the greatest attention. The present study was undertaken to evaluate the in vitro and in vivo antioxidant activities of aqueous extract of Artemisia vulgaris. The plant extract was tested for DPPH (2, 2-diphenyl, 2-picryl hydrazyl) radical scavenging, nitric oxide radical scavenging, reducing power assays, total phenol, flavonoid and flavonol content. Determination of serum ascorbic acid level, blood glutathione level and superoxide dismutase activity in rats treated with 100 mg/Kg of Artemisia vulgaris extract. The extract exhibited scavenging potential with IC50 value of 11.4 micro g/ ml for DPPH, the value were found to close to those of standard rutin (10 micro g/ ml).On the other hand Artemisia vulgaris extract exhibited nitric oxide scavenging activity with IC50 value 125 mg/ ml. The reducing power of the extract depends on the amount of extract. The content of phenolic compounds (mg/g) in aqueous extract was found 19 +/- 0.16 mg/g plant extract and expressed in gallic acid equivalents (GAE). The flavonoidal and flavonol contents were found to be 7.96 +/- 0.76 and 3.4 +/- 0.0 respectively mg/g plant extract in rutin equivalent. The treatment of rats with aqueous extract of Artemisia vulgaris resulted in a significant increase in blood glutathione level, superoxide dismutase activity and serum ascorbic acid level as compared to their corresponding controls. The results obtained in the present study indicate that aqueous extract of Artemisia vulgaris is a potential source of natural antioxidants.

  18. Study of antioxidant activity of natural food supplements

    Directory of Open Access Journals (Sweden)

    Tetyana Lozova

    2013-11-01

    Full Text Available 96 Normal 0 false false false CS JA X-NONE This article describes the results of a study of antioxidant activity of natural food supplements suggested for use in flour confectionery production. Oxidation rate of the model substance - cumene - was measured using a volumetric unit. Diagram of absorbed oxygen amount as a function of time (∆HO2 over t was built by measuring time in minutes and absorbed oxygen volume in cm3. This diagram was subsequently used to graphically determine the oxidation rate as the slope ratio of the line in specified coordinates. Afterwards, the oxidation rate was measured at a different initiation rate (different azobisisobutyronitrile solution volume, while all other parameters of the experiment remained unaltered. On the basis of the resulting data, diagrams of oxidation rate as a function of initiation rate were built for all investigated substances (both extracts and powders. The study revealed that apian products, including pollen and propolis, as well as kidney bean powder and phytosupplements (leaves of leather bergenia, lime blossom, heartsease, wild chamomile, pepper mint, bog rosemary, and elderflowers, possessed high antioxidant activity. According to the research data, the highest activity was detected in propolis  0.482·20 pollen 0.802 and powdered forms of pepper mint 1.066 leather bergenia leaves 0.937 heartsease 0.385 lime blossom 0.331 and kidney beans 0.323. Relatively lower antioxidant activity was found in powdered bog rosemary 0.242 elderflowers 0.238 and wild chamomile 0.212. (Introduction of the investigated supplements will allow inhibiting oxidation processes in the lipide fraction of foodstuffs, including flour confectionery, to ensure stability of their qualitative characteristics over a longer period.

  19. A GC-MS study of the volatile organic composition of straw and oyster mushrooms during maturity and its relation to antioxidant activity.

    Science.gov (United States)

    Zhang, Zhuo-Min; Wu, Wen-Wei; Li, Gong-Ke

    2008-09-01

    Mushrooms are very popular in the market for their nutritional and medicinal use. Mushroom volatiles are not only an important factor in the flavor, but also contain many antioxidant compounds. Antioxidant activity is a very important property for disease prevention. The volatile compositional characteristics of straw mushrooms (Volvariella volvacea [Bull. ex Fr.] Sing.) and oyster mushrooms (Pleurotus ostreatus [Jacq. ex Fr.] Kummer) during maturity and the mushroom antioxidant activity related to the non-volatiles and volatiles are studied by a chromatographic method in combination with a spectrophotometric method. The volatile compounds of straw and oyster mushrooms are sampled and identified by a combination sampling method, including headspace solid phase microextraction and steam distillation, followed by gas chromatography-mass spectrometry detection. Among all the volatile compounds identified, 1-octen-3-ol and 3-octanone are the two main compounds with the highest amounts in the volatile compositions of straw and oyster mushrooms. During maturity time of the straw mushrooms, the unsaturated 1-octen-3-ol peak area is reduced, whereas the saturated 3-octanone peak area is increased. However, during normal maturity time of oyster mushrooms, the peak areas of 1-octen-3-ol and 3-octanone remain at the same level. 1-Octen-3-ol has a different antioxidant activity from 3-octanone. Combining the results of antioxidant experiments of water extract and main volatile components by the use of a phosphomolybdenum spectrophotometric method, the conclusion is drawn that oyster mushrooms might possess stronger antioxidant activities than straw mushrooms.

  20. Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco

    Directory of Open Access Journals (Sweden)

    Naima Chahmi

    2015-03-01

    Conclusions: Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants. The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.

  1. Radiation chemistry of some novel antioxidants from turmeric and other natural products: physico-chemistry properties and antioxidant activities

    International Nuclear Information System (INIS)

    Indira Priyadarsini, K.; Guha, S.N.; Rao, M.N.A.

    1998-01-01

    Using pulse radiolysis the free radical reactions of the structurally related phenols were studied with biologically relevant oxidants both in aqueous-organic solutions and neutral surfactant solutions. The resulting phenoxyl radicals were characterised by their lifetimes, absorption spectra, one-electron reduction potential and reaction with other antioxidants. The physicochemical properties of the phenoxyl radicals were compared with the antioxidant activities of these compounds

  2. Lifestyle predictors of oxidant and antioxidant enzyme activities and total antioxidant capacity in healthy women: a cross-sectional study.

    Science.gov (United States)

    Mahasneh, Amjad A; Zhang, Yali; Zhao, Hua; Ambrosone, Christine B; Hong, Chi-Chen

    2016-12-01

    The aim of this study was to identify demographic and modifiable lifestyle factors that may be related to endogenous oxidant and antioxidant activity measured in blood specimens from putatively healthy women recruited at the Roswell Park Cancer Institute (Buffalo, NY, USA). Total glutathione (TGSH), catalase (CAT), CuZn-superoxide dismutase (CuZn-SOD), glutathione peroxidase (GPx), glutathione reductase (GR), and myeloperoxidase (MPO) activity, and total antioxidant capacity (TAC) were measured in 124 healthy women, and associations with epidemiological factors were tested using general linear models. There were significant differences in oxidant and antioxidant enzyme activities according to lifestyle factors, after adjusting for duration of blood storage and season of blood draw. Compared to women who consumed ≤2.8 servings of fruits and vegetables daily, those consuming >5.3 servings had on average 31 % lower MPO activity (p-trend = 0.02), as a marker of oxidative stress, 16 % higher antioxidant GPx activity (p-trend = 0.08), and 9 % higher TAC (p-trend = 0.05). Obese women (body mass index, BMI ≥ 30) in contrast showed 17 % lower antioxidant GPx activity, 44 % higher MPO activity (p-trend = 0.03), and 10 % higher TAC (p-trend = 0.03) compared to women with normal BMI lifestyle factors and, therefore, may be potentially modifiable, with implications for risk reduction of chronic conditions related to oxidative stress.

  3. GC-MS analysis of the antioxidant active fractions of Micromeria juliana with anticholinesterase activity.

    Science.gov (United States)

    Oztürk, Mehmet; Kolak, Ufuk; Duru, Mehmet Emin; Harmandar, Mansur

    2009-09-01

    The aerial parts of Micromeria juliana (L.) Bentham ex Reichb. were extracted with light petroleum, acetone and methanol, successively. The antioxidant activity of different concentrations of the extracts was evaluated using different antioxidant tests, namely total antioxidant (lipid peroxidation inhibition activity), DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, ferric reducing power, and metal chelating. Total antioxidant activity was determined using the beta-carotene-linoleic acid assay. Unexpectedly, the light petroleum extract exhibited strong lipid peroxidation inhibition activity. The extract was fractionated on a silica gel column and the antioxidant activity of the fractions was determined by the beta-carotene-linoleic assay at 25 microg/mL concentration. The fractions that exhibited more than 50% inhibition activity were analysed by GC and GC/MS; thus, the structure of fourteen compounds were elucidated. In addition, acetyl- and butyryl-cholinesterase inhibitory activities of the extracts were also determined in vitro. The light petroleum and acetone extracts were found to have mild butyrylcholinesterase inhibitory activity.

  4. Antioxidant activities of aqueous extracts from 12 Chinese edible flowers in vitro and in vivo

    OpenAIRE

    Wang, Feng; Miao, Miao; Xia, Hui; Yang, Li-Gang; Wang, Shao-Kang; Sun, Gui-Ju

    2016-01-01

    ABSTRACT The antioxidant function of edible flowers have attracted increasing interest. However, information is lacking on the impact of edible flowers on oxidative injury including hypoxia-re-oxygenation and hyperlipidemia. The antioxidant activities of aqueous extracts from 12 Chinese edible flowers were assessed in four different antioxidant models, including total antioxidant capacity (TAC), oxygen radical absorbance capacity (ORAC), scavenging hydroxyl radical capacity (SHRC) and scaveng...

  5. Antioxidant, Antimicrobial and Antiproliferative Activities of Five Lichen Species

    Science.gov (United States)

    Mitrović, Tatjana; Stamenković, Slaviša; Cvetković, Vladimir; Tošić, Svetlana; Stanković, Milan; Radojević, Ivana; Stefanović, Olgica; Čomić, Ljiljana; Đačić, Dragana; Ćurčić, Milena; Marković, Snežana

    2011-01-01

    The antioxidative, antimicrobial and antiproliferative potentials of the methanol extracts of the lichen species Parmelia sulcata, Flavoparmelia caperata, Evernia prunastri, Hypogymnia physodes and Cladonia foliacea were evaluated. The total phenolic content of the tested extracts varied from 78.12 to 141.59 mg of gallic acid equivalent (GA)/g of extract and the total flavonoid content from 20.14 to 44.43 mg of rutin equivalent (Ru)/g of extract. The antioxidant capacities of the lichen extracts were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals scavenging. Hypogymnia physodes with the highest phenolic content showed the strongest DPPH radical scavenging effect. Further, the antimicrobial potential of the lichen extracts was determined by a microdilution method on 29 microorganisms, including 15 strains of bacteria, 10 species of filamentous fungi and 4 yeast species. A high antimicrobial activity of all the tested extracts was observed with more potent inhibitory effects on the growth of Gram (+) bacteria. The highest antimicrobial activity among lichens was demonstrated by Hypogymnia physodes and Cladonia foliacea. Finally, the antiproliferative activity of the lichen extracts was explored on the colon cancer adenocarcinoma cell line HCT-116 by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) viability assay and acridine orange/ethidium bromide staining. The methanol extracts of Hypogymnia physodes and Cladonia foliacea showed a better cytotoxic activity than the other extracts. All lichen species showed the ability to induce apoptosis of HCT-116 cells. PMID:21954369

  6. Antioxidant, Antimicrobial and Antiproliferative Activities of Five Lichen Species

    Directory of Open Access Journals (Sweden)

    Snežana Marković

    2011-08-01

    Full Text Available The antioxidative, antimicrobial and antiproliferative potentials of the methanol extracts of the lichen species Parmelia sulcata, Flavoparmelia caperata, Evernia prunastri, Hypogymnia physodes and Cladonia foliacea were evaluated. The total phenolic content of the tested extracts varied from 78.12 to 141.59 mg of gallic acid equivalent (GA/g of extract and the total flavonoid content from 20.14 to 44.43 mg of rutin equivalent (Ru/g of extract. The antioxidant capacities of the lichen extracts were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH radicals scavenging. Hypogymnia physodes with the highest phenolic content showed the strongest DPPH radical scavenging effect. Further, the antimicrobial potential of the lichen extracts was determined by a microdilution method on 29 microorganisms, including 15 strains of bacteria, 10 species of filamentous fungi and 4 yeast species. A high antimicrobial activity of all the tested extracts was observed with more potent inhibitory effects on the growth of Gram (+ bacteria. The highest antimicrobial activity among lichens was demonstrated by Hypogymnia physodes and Cladonia foliacea. Finally, the antiproliferative activity of the lichen extracts was explored on the colon cancer adenocarcinoma cell line HCT-116 by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide viability assay and acridine orange/ethidium bromide staining. The methanol extracts of Hypogymnia physodes and Cladonia foliacea showed a better cytotoxic activity than the other extracts. All lichen species showed the ability to induce apoptosis of HCT-116 cells.

  7. Antioxidant Activity of Phytic Acid in Lipid Model System

    Directory of Open Access Journals (Sweden)

    Marijana Sakač

    2010-01-01

    Full Text Available Free radicals formed during thermal (60 °C, 24 h and catalytic (Fe2+ ions, room temperature (23±1 °C, 3 h oxidative degradation of hydroperoxyde-enriched soybean oil (HESO were stabilized in the presence of spin trap N-tert-butyl-α-phenylnitrone (PBN and detected by electron spin resonance (ESR spectrometry. In both thermal and catalytic oxidation of HESO, the same hyperfine coupling parameters (aN=14.75 G and aH β=2.80 G confirmed the generation of PBN-OOL/-OL spin adducts. The antiradical activity (AA of phytic acid, in the 0.076–0.30 mM concentration range, was tested by measuring its ability to inhibit the generation of PBN-OOL/-OL spin adducts during thermal and catalytic oxidation of HESO. Phytic acid did not inhibit the thermal oxidation of HESO and showed no effect in the β-carotene bleaching test (AOA. Contrary to this, phytic acid exhibited antioxidant effect on the catalytic oxidation of HESO by chelating Fe2+ ions. The mechanism of antioxidant activity was confirmed by the results of chelating activity on Fe2+ in Fe2+-ferrozine test where a dose-dependent chelating activity of phytic acid was obtained.

  8. Antimicrobial, antioxidant activities and chemical composition of selected Thai spices

    Directory of Open Access Journals (Sweden)

    Juraithip Wungsintaweekul

    2010-12-01

    Full Text Available Nine volatile oils and six methanol extracts from Ocimum americanum, O. basilicum, O. sanctum, Citrus hystrix,Alpinia galanga, Curcuma zedoaria, Kaempferia parviflora and Zingiber cassumunar were assessed for antimicrobial andantioxidant activities. The volatile oils and extracts were investigated against eight bacteria and three fungi. The resultsillustrated that O. americanum volatile oil exhibited broad spectrum activity against tested bacteria with the MICs ranging1.4-3.6 mg/ml and Candida spp. with the MICs ranging from 0.5-0.6 mg/ml. The O. sanctum volatile oil showed a considerableactivity against only Candida spp. with the MICs ranging from 0.8-1.4 mg/ml. Interestingly, growth of Mycobacteriumphlei was inhibited by the volatiles of O. americanum, C. hystrix peel, and C. zedoaria with MIC of 1.7, 3.5 and 1.2 mg/ml,respectively. For antioxidant activity evaluation, the methanol extracts of C. hystrix (leaf and peel and K. parviflora hadpotent antioxidant activity by the radical-scavenging DPPH method with IC50 of 24.6, 66.3 and 61.5 mg/ml, respectively.GC-MS analysis revealed the typical chemical profiles of the volatile oils. The major component showed the characteristicsof the volatile oils and was probably responsible for the antimicrobial effect.

  9. Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica

    Directory of Open Access Journals (Sweden)

    PEIYUAN LI

    2011-05-01

    Full Text Available Pouzolzia zeylanica was extracted with different solvents (acetone, ethyl acetate and petroleum ether, using different protocols (cold-extraction and Soxhlet extraction. To evaluate the antiradical and antioxidant abilities of the extracts, four in vitro test systems were employed, i.e., DPPH, ABTS and hydroxyl radical scavenging assays and a reducing power assay. All extracts exhibited outstanding antioxidant activities that were superior to that of butylated hydroxytoluene. The ethyl acetate extracts exhibited the most significant antioxidant activities, and cold-extraction under stirring seemed to be the more efficacious method for acquiring the predominant antioxidants. Furthermore, the antioxidant activities and total phenolic (TP content of different extracts followed the same order, i.e., there is a good correlation between antioxidant activities and TP content. The results showed that these extracts, especially the ethyl acetate extracts, could be considered as natural antioxidants and may be useful for curing diseases arising from oxidative deterioration.

  10. Improving the capacity of polypropylene to be used in antioxidant active films: incorporation of plasticizer and natural antioxidants.

    Science.gov (United States)

    Castro López, María del Mar; López de Dicastillo, Carol; López Vilariño, José Manuel; González Rodríguez, María Victoria

    2013-09-04

    Two types of active antioxidant food packages with improved release properties, based on polypropylene (PP) as one of the most common polymers used in food-packaging applications, were developed. Incorporation of catechin and green tea as antioxidant provided PP with 6 times higher stabilization against thermal oxidation. Release of natural antioxidants (catechins, gallic acid, caffeine, and quercetin) into various food simulants from that nonpolar matrix were improved by blending poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol) (PPG-PEG-PPG) as plasticizer into the polymer formulation. Increasing release levels between 10- and 40-fold into simulant A and between 6 and 20-fold into simulant D1 resulted from the incorporation of catechin and green tea as antioxidants and PPG-PEG-PPG as plasticizer into the film formulation. The efficiency of the antioxidants in the food simulants after the release process was also corroborated through antioxidant activity tests. Therefore, the developed PPG-PEG-PPG-modified polypropylene resulted in a potential system to be used in active packaging.

  11. Antioxidant activity and calcium binding of isomeric hydroxybenzoates

    Directory of Open Access Journals (Sweden)

    Zichen Zhao

    2018-04-01

    Full Text Available The association constant for calcium binding to hydroxybenzoates in aqueous 0.16 M NaCl at 25 °C was found electrochemically to have the value Kass = 280 mol L−1 with ΔHo = −51 kJ mol−1, ΔSo = −122 J mol−1 K−1 for the 2-isomer (salicylate, Kass = 7 mol L−1 with ΔHo = −39 kJ mol−1, ΔSo = −116 J mol−1 K−1 for the 3-isomer, and Kass = 8 mol L−1 with ΔHo = −51 kJ mol−1, ΔSo = −155 J mol−1 K−1 for the 4-isomer. The 3- and 4-isomers were found more efficient as antioxidants than the 2-isomer in decreasing oxygen consumption rate in a peroxidating methyl linoleate emulsion and less sensitive to presence of calcium. All isomers were found prooxidative for iron-catalyzed initiation of oxidation due to enhanced radical formation as shown by electron spin resonance spectroscopy. Calcium salicylate was found to have low solubility with a solubility product Ksp = 4.49·10−6 based on activity with ΔHo = 67 kJ mol−1, ΔSo = 123 J mol−1 K−1 for dissolution in water, when corrected for the strong complex formation. Calcium in food and beverages may thus lower antioxidant activity of plant phenols through complexation or by precipitation. Keywords: Antioxidant activity, Calcium binding, 2-Hydroxybenzoate, 3-Hydroxybenzoate, 4-Hydroxybenzoate

  12. Antioxidant Activity of Flaxseed Extracts in Lipid Systems

    Directory of Open Access Journals (Sweden)

    Adriana Slavova-Kazakova

    2015-12-01

    Full Text Available The aim of this work was to compare the antioxidant activity of the extract of flaxseed and its alkaline hydrolysate in two model systems: lipid autoxidation of triacylglycerols of sunflower oil (TGSO—in a homogeneous lipid media and during β-carotene-linoleate emulsion system. In addition, pure lignans were tested. The material was defatted with hexane and then phenolic compounds were extracted using dioxane-ethanol (50:50, v/v mixture. Carbohydrates were removed from the crude extract using an Amberlite XAD-16 column chromatography. The content of total phenolic compounds in the crude extract and after alkaline hydrolysis was determined using a Folin-Ciocalteu’s phenol reagent. Individual phenolic compounds were determined by nordihydroguaiaretic acid (RP-HPLC method in gradient system. The alkaline hydrolysis increased the content of total phenolics in the extract approximately by 10%. In the extracts of flaxseed, phenolic compounds were present in the form of macromolecular complex. In the alkaline hydrolysate, secoisolariciresinol diglucoside (SDG was found as the main phenolic compound. Small amounts of p-coumaric and ferulic acids were also determined. SDG and both extracts were not able to inhibit effectively lipid autoxidation. The kinetics of TGSO autoxidation at 80 °C in absence and in presence of the extract before hydrolysis (EBH and after hydrolysis (EAH was monitored and compared with known standard antioxidants. Ferulic acid (FA and butylated hydroxyl toluene (BHT showed much higher antioxidant efficiency and reactivity than that of both extracts. Secoisolariciresinol (SECO showed a higher activity in both model systems than SDG. However, the activity of SECO was much lower than that of nordihydroquaiaretic acid (NDGA.

  13. Olive oils from Algeria: Phenolic compounds, antioxidant and antibacterial activities

    Directory of Open Access Journals (Sweden)

    Laincer, F.

    2014-03-01

    Full Text Available The phenolic compositions, antioxidant and antimicrobial activities against six bacteria of phenolic extracts of olive oil varieties from eleven Algerian varieties were investigated. The antioxidant activity was assessed by determining the scavenging effect on the DPPH and ABTS.+ radicals. The antimicrobial activity was measured as a zone of inhibition and minimum inhibitory concentration (MIC on human harmful and foodborne pathogens. The results show that total phenols was significantly (p .+ radicals (r = 0.76. Among the bacteria tested, S. aureus and to a lesser extent B. subtilis showed the highest sensitivity; the MIC varied from 0.6 to 1.6 mg·mL-1 and 1.2 to 1.8 mg·mL-1, respectively. The results reveal that Algerian olive oils may constitute a good source of antioxidant and antimicrobial agents.Se ha estudiado la composición fenólica y las actividades antioxidante y antimicrobiana, contra seis bacterias, de extractos de aceites de oliva de once variedades argelinas. La actividad antioxidante se evaluó mediante la determinación del efecto captador de radicales de DPPH y ABTS.+. La actividad antimicrobiana se midió como zona de inhibición y como concentración inhibitoria mínima (MIC sobre bacterias perjudiciales humanas y agentes patógenos transmitidos por los alimentos. Los resultados mostraron que los fenoles totales está significativamente (p .+ (r= 0,76. Entre las bacterias ensayadas, S. aureus y, en menor grado B. subtilis mostraron la mayor sensibilidad; el MIC varió de 0,6 a 1,6 mg·mL-1 y 1,2 a 1,8 mg·mL-1 respectivamente. Los resultados muestran que los aceites de oliva argelinos pueden constituir una buena fuente de antioxidantes y agentes antimicrobianos.

  14. Synthesis and antioxidant activity evaluations of melatonin-based analogue indole-hydrazide/hydrazone derivatives.

    Science.gov (United States)

    Yılmaz, Ayse Didem; Coban, Tulay; Suzen, Sibel

    2012-06-01

    Melatonin (MLT) is a hormone synthesized from the pineal gland. It is a direct scavenger of free radicals, which is related to its capability to defend cells from oxidative stress. Recently MLT-related compounds are under investigation to establish which exhibit the maximum activity with the lowest side effects. In this study 5-chloroindole hydrazide/hydrazone derivatives were synthesized from 5-chloroindole-3-carboxaldehyde and phenyl hydrazine derivatives. All the compounds characterized and in vitro antioxidant activity was investigated against MLT and BHT. Most of the compounds showed strong inhibitory effect on the superoxide radical scavenging assay at 1 mM concentration (79 to 95%). Almost all the tested compounds possessed strong scavenging activity against the DPPH radical scavenging activity with IC(50) values (2 to 60 µM). Lastly, compound 1j revealed stronger inhibitory activity against MLT in the LP inhibitory assay at 0.1mM concentration (51%) while the rest of the compounds showed moderate inhibition.

  15. Phytochemical Compositions and Antioxidant and Anti-Inflammatory Activities of Crude Extracts from Ficus pandurata H. (Moraceae)

    Science.gov (United States)

    Lv, Huiqing; Zhang, Xiaoping; Chen, XueZhi; Xie, Zhijun; Wen, Chengping; Jiang, Kezhi

    2013-01-01

    Background. Ficus pandurata H. (Moraceae) is widely used in traditional Chinese medicine as a healthy food condiment or a medicine for treatment of various diseases including inflammation. Objective. The purpose of the present study is to investigate the phytochemical compositions and antioxidant and anti-inflammatory activities of crude water (FPW) and ethanolic extracts (FPE) from Ficus pandurata H. Methods. Phytochemical compositions were identified by a high-performance liquid chromatography-electrospray ionization-mass spectrometry method (HPLC-ESI-MS). The antioxidant activities were evaluated by diphenylpicrylhydrazyl (DPPH) and hydroxyl radical assays, and the anti-inflammatory activities were evaluated by paw edema and levels of inflammatory mediator TNF-α and PGE2 in monosodium urate (MSU) crystal-induced rats. Results. Six compounds were identified by HPLC-MS method, and abundance of phenolics was found in FPE. The FPE showed concentration-dependent-significant scavenging of DPPH and hydroxyl radicals with IC50 values 118.4 and 192.9 μg/mL, respectively. The FPE treatment significantly inhibited the paw edema and the production of TNF-α and PGE2 in MSU crystal-induced rats. Conclusion. The FPE exerted stronger antioxidant and anti-inflammatory activities which may be attributed to its high phenolic content. PMID:24191163

  16. Chemical properties and antioxidant activity of exopolysaccharides fractions from mycelial culture of Inonotus obliquus in a ground corn stover medium.

    Science.gov (United States)

    Xiang, Yuling; Xu, Xiangqun; Li, Juan

    2012-10-15

    The medicinal mushroom Inonotus obliquus has been a folk remedy for a long time in East-European and Asian countries. We first reported the enhancement in production and antioxidant activity of exopolysaccharides by I. obliquus culture under lignocellulose decomposition. In this study, the two different sources of exopolysaccharides from the control medium and the lignocellulose (corn stover) containing medium by I. obliquus in submerged fermentation were fractionated and purified by chromatography. The exopolysaccharides from the corn stover-containing medium presented significantly stronger hydroxyl and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity than the control. Three fractions from the control medium and the corn stover-containing medium were isolated respectively. The fraction of DEPL3 from the corn stover-containing medium with the highest protein content (38.3%), mannose content (49.6%), and the lowest molecular weight (29 kDa) had the highest antioxidant activity with the lowest IC50 values. In conclusion, lignocellulose decomposition changed the chemical characterisation and significantly enhanced the antioxidant activity of exopolysaccharide fractions. Copyright © 2012 Elsevier Ltd. All rights reserved.

  17. Antioxidant Capacity and Superoxide Dismutase Activity in Adrenoleukodystrophy.

    Science.gov (United States)

    Turk, Bela R; Theisen, Benjamin E; Nemeth, Christina L; Marx, Joel S; Shi, Xiaohai; Rosen, Melissa; Jones, Richard O; Moser, Ann B; Watkins, Paul A; Raymond, Gerald V; Tiffany, Carol; Fatemi, Ali

    2017-05-01

    X-linked adrenoleukodystrophy (ALD) may switch phenotype to the fatal cerebral form (ie, cerebral ALD [cALD]), the cause of which is unknown. Determining differences in antioxidant capacity and superoxide dismutase (SOD) levels between phenotypes may allow for the generation of a clinical biomarker for predicting the onset of cALD, as well as initiating a more timely lifesaving therapy. To identify variations in the levels of antioxidant capacity and SOD activity between ALD phenotypes in patients with cALD or adrenomyeloneuropathy (AMN), heterozygote female carriers, and healthy controls and, in addition, correlate antioxidant levels with clinical outcome scores to determine a possible predictive value. Samples of monocytes and blood plasma were prospectively collected from healthy controls, heterozygote female carriers, and patients with AMN or cALD. We are counting each patient as 1 sample in our study. Because adrenoleukodystrophy is an X-linked disease, the affected group populations of cALD and AMN are all male. The heterozygote carriers are all female. The samples were assayed for total antioxidant capacity and SOD activity. The data were collected in an academic hospital setting. Eligibility criteria included patients who received a diagnosis of ALD and heterozygote female carriers, both of which groups were compared with age-matched controls. The prospective samples (n = 30) were collected between January 2015 to January 2016, and existing samples were collected from tissue storage banks at the Kennedy Krieger Institute (n = 30). The analyses were performed during the first 3 months of 2016. Commercially available total antioxidant capacity and SOD assays were performed on samples of monocytes and blood plasma and correlated with magnetic resonance imaging severity score. A reduction in antioxidant capacity was shown between the healthy controls (0.225 mmol trolox equivalent) and heterozygote carriers (0.181 mmol trolox equivalent), and significant

  18. Antioxidant and cytotoxic activities investigation of tomato seed extracts.

    Science.gov (United States)

    Tommonaro, Giuseppina; Caporale, Angelamaria; De Martino, Laura; Popolo, Ada; De Prisco, Rocco; Nicolaus, Barbara; Abbamondi, Gennaro Roberto; Saturnino, Carmela

    2014-01-01

    Biological activities of different varieties of tomato seed extracts were evaluated to verify the potential antioxidant and/or antiproliferative activity of the bioactive metabolites present in them. Findings demonstrated that among all the varieties investigated (San Marzano Rosso, San Marzano Giallo, Corbarino, Black Tomato and San Marzano/Black Tomato hybrid) San Marzano Rosso seed extract exhibited the highest free radical-scavenging activity with 68% of 2,2-Diphenyl-1-picrylhydrazyl radical inhibition, and the best cytotoxic activity evaluated by using the brine shrimp test (LD50: 23,198 ppm) and 3-(4,5-dimethylthiazol-2-yl)-2,5-phenyl-2H-tetrazolium bromide assay on A375 cell line (IC50: 137.7 μg/mL).

  19. ANTIOXIDANT ENZYME ACTIVITY AND FRESH-CUT ARRACACHA QUALITY

    Directory of Open Access Journals (Sweden)

    Hêmina Carla Vilela

    2015-06-01

    Full Text Available The arracacha is an alternative of fresh-cut product; however it can be easily degraded after the processing techniques. The objective of this work was to evaluate the useful life of fresh-cut arracacha submitted to two types of cuts and storage, as well as to evaluate the activity of antioxidant enzymes. The roots were selected, sanitized and submitted to two cut types: cubed and grated. Then they were evaluated at 3 times: 0, 3 and 7 days. The cutting in cubes provided higher quality and lower SOD, CAT and APX activity. However, the grated product presented higher PG activity and lower PPO activity. The microbiological safety and the nutritional value were maintained in both cuts during the whole storage period. The useful life, regarding the physicochemical, nutritional and microbiological aspects, can be established at 7 days under refrigeration for fresh-cut arracacha.

  20. Antioxidant activity and phytochemical compounds of snake fruit (Salacca Zalacca)

    Science.gov (United States)

    Suica-Bunghez, I. R.; Teodorescu, S.; Dulama, I. D.; Voinea, O. C.; imionescu, S.; Ion, R. M.

    2016-06-01

    Snake fruit (Salacca zalacca) is a palm tree species, which is found in Malaysia and Indonesia. This study was conducted to investigate and compare the composition, total phenolic, flavonoid, tanins and monoterpenoids contents in the core and shell fruits. Concentration values of extracts were obtained from standard curves obtained. Antioxidant activity was determined using DPPH method. For all methods it was used the UV-VIS Specord M40, using different wavelength. The infrared spectral analysis was carried out to caracterized the type of functional group existent in snake fruit parts (shell and core).

  1. Chemical reaction due to stronger Ramachandran interaction

    Indian Academy of Sciences (India)

    The origin of a chemical reaction between two reactant atoms is associated with the activation energy, on the assumption that, high-energy collisions between these atoms, are the ones that overcome the activation energy. Here, we show that a stronger attractive van der Waals (vdW) and electron-ion Coulomb interactions ...

  2. STRUCTURE – ANTIOXIDANT ACTIVITIES RELATIONSHIP ANALYSIS OF ISOEUGENOL, EUGENOL, VANILIN AND THEIR DERIVATIVES

    Directory of Open Access Journals (Sweden)

    Nur Aini

    2010-06-01

    Full Text Available Structure Activity Relationship (SAR technique between the theoretical parameters and antioxidant activities of isoeugenol, eugenol, vanillin and their derivatives as Mannich reaction products, have been analyzed. Antioxidant activities were examined by oxidation reaction of oleic acid at 60 °C with b-carotene methods, whereas theoretical parameters of the activities were determined by calculating Bonding Dissociation Enthalpy (BDE and net charge of oxygen atom(-OH using AM1 semi empiric methods. The result from both test showed in the following orders: BHT > Mannich product of isoeugenol > isoeugenol > Mannich product of eugenol > eugenol > Mannich product of vanillin > vanillin. The antioxidant activities increase with small the BDE value and high the net charge. Electron donating groups will increase the antioxidants activity with lowering the BDE value and increasing the net charge, while electron-withdrawing groups will decrease antioxidants activity.   Keywords: SAR, antioxidants, Bonding Dissociation Entalphy, eugenol.

  3. Chemical composition, antimicrobial and antioxidant activity of essential oils from cumin and ajowan

    Directory of Open Access Journals (Sweden)

    SAHADEO D. PATIL

    2016-01-01

    Full Text Available Abstract. Patil SD, Maknikar PP, Wankhade SJ, Ukesh CS, Rai MK. 2016. Chemical composition, antimicrobial and antioxidant activity of essential oils from cumin and ajowan. Nusantara Bioscience 8: 60-65. Plant essential oils have gained importance as alternative remedies for treatment of many infectious diseases and food preservatives. In the present study, we have determined the chemical composition of the essential oils (EOs from two Indian spices Cuminum cyminum (cumin and Trachyspermum ammi (ajowan of family Apiaceae by gas chromatography-mass spectrometry (GC-MS. Moreover, the antimicrobial potential of these oils was evaluated against several Gram-positive and Gram-negative bacteria using disc diffusion and broth microdilution methods. A Total of 20 major chemical components were analyzed by GC-MS studies and were found to be cuminaldehyde (36.67% and caren-10-al (21.34% in case of cumin essential oil while p-cymene (15.54% and thymol (15.48% were found to be present in ajowan essential oil. Both the EOs exhibited potent antibacterial effect against most of the tested pathogens. Furthermore, cumin and ajowan EOs demonstrated remarkable antibacterial activity against Salmonella typhi with an inhibition zone diameter of 54 and 60 mm respectively with identical MIC value of 12.5 µl/ml. Ajowan EO was found to exhibit wide spectrum activity against both the Gram-positive and Gram-negative organisms when compared with cumin. Both the essential oils were more potent than standard antibiotic chloramphenicol except cumin against Escherichia coli and Enterobacter aerogenes. Antioxidant activity of cumin was weaker (12.36% and ajowan was stronger (71.68% than standard ascorbic acid (20.24% at 1000 µg/ml concentration when assessed by DPPH radical scavenging assay. Our study suggests that, spice essential oils have significant potential in controlling the human and foodborne pathogens.

  4. Antioxidant Activity of Spices and Their Impact on Human Health: A Review.

    Science.gov (United States)

    Yashin, Alexander; Yashin, Yakov; Xia, Xiaoyan; Nemzer, Boris

    2017-09-15

    Antioxidants are substances that prevent oxidation of other compounds or neutralize free radicals. Spices and herbs are rich sources of antioxidants. They have been used in food and beverages to enhance flavor, aroma and color. Due to their excellent antioxidant activity, spices and herbs have also been used to treat some diseases. In this review article, the chemical composition and antioxidant activity of spices and culinary herbs are presented. The content of flavonoids and total polyphenols in different spices and herbs are summarized. The applications of spices and their impacts on human health are briefly described. The extraction and analytical methods for determination of antioxidant capacity are concisely reviewed.

  5. Isolation, Identification and Activities of Natural Antioxidants from Callicarpa kwangtungensis Chun

    Science.gov (United States)

    Cai, Hao; Xie, Zhiyong; Liu, Guanghui; Sun, Xiuman; Peng, Guangtian; Lin, Baoqin; Liao, Qiongfeng

    2014-01-01

    Reactive oxygen species leads to some diseases associated with oxidative stress. Callicarpa kwangtungensis Chun (CK) is a common remedy in traditional Chinese medicine and possesses diverse biological activities involving antioxidant properties; its main compounds phenylethanoid glycosides (PG) and flavonoids are always reported as antioxidants. In order to develop CK as a safe and activated antioxidant, our investigation was performed to validate antioxidant properties and assess which types of compounds (similar polarity or similar structure), even which compounds, played the role of antioxidants. The extracted compounds of CK were analyzed qualitatively and quantitatively by HPLC-DAD-ESI-Trap MS and UV for their contents and antioxidant activities. The correlations between antioxidant activities and known contents were respectively counted and a semi-quantitative experiment was designed to screen antioxidant compounds of CK with HPLC-UV. The n-butanol fraction (BF) showed the highest total phenolic and flavonoid contents (TPC, TFC), and three PG (forsythiaside B, poliumoside and acteoside) contents. BF showed the significantly best (Pantioxidant activities on CCl4-induced acute liver injury Mice and TBHP-reduced HepG2 cells. Nine PG (forsythiaside B, poliumoside, acteoside, alyssonoside, brandioside and their derivatives) and one flavone (rhamnazin) were screened out as antioxidants. BF in CK contained abundant polyphenolic, which reflected some definite antioxidant properties. The antioxidant compounds consisted at the least of nine PG and one flavone. PMID:24667350

  6. Hepatoprotective and antioxidant activities of Tamarix nilotica flowers.

    Science.gov (United States)

    Abouzid, Sameh; Sleem, Amany

    2011-04-01

     Tamarix nilotica (Ehrenb.) Bunge (Tamaricaceae) is used in the Egyptian traditional medicine as an antiseptic agent. This plant has been known since pharaonic times and has been mentioned in medical papyri to expel fever, relieve headache, to draw out inflammation, and as an aphrodisiac. No scientific study is available about the biological effect of this plant.  This study aimed to evaluate the hydro-alcoholic extract (80%) of T. nilotica flowers for hepatoprotective and antioxidant activities.  Hepatoprotective activity was assessed using carbon tetrachloride-induced hepatic injury in rats by monitoring biochemical parameters. Antioxidant activity was evaluated in alloxan-induced diabetic rats. Biochemical markers of hepatic damage such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), and tissue glutathione were determined in all groups.  Carbon tetrachloride (5 mL/kg body weight) enhanced the SGOT, SGPT, and ALP levels. There was a marked reduction in tissue glutathione level in diabetic rats. The hydro-alcoholic extract of T. nilotica (100 mg/kg body weight) ameliorated the adverse effects of carbon tetrachloride and returned the altered levels of biochemical markers near to the normal levels.

  7. Erythrocyte membrane stabilization effect and antioxidant activity of methyl methacrylate

    International Nuclear Information System (INIS)

    Popov, B.

    2004-01-01

    Methyl methacrylate (MMK) is a synthetic product with mild impact on human health that is not well studied on cellular basis. Here, human erythrocytes were used to investigate the effects MMK exerts on acid and heat-induced hemolysis. Biphasic effect of MMK was observed for acid-induced hemolysis; i.e., protection at low (0 - 0.05% v/v) and stimulation at higher (0.1- 0.4% v/v) concentrations. The maximal protective effect was produced at 0.03% (v/v). At this concentration MMK increased the temperatures of heat denaturation of erythrocyte membrane proteins, spectrin and integral proteins, by about 2 0 C and inhibited the heat-induced hemolysis by 20 %. This membrane stabilization effect of MMK is similar to that produced by some anti-inflammatory and antirheumatic drugs. The increased acid resistance possibly indicated anti-oxidant properties of MMK. The nonenzymatic antioxidant activity test evidenced that MMK has no superoxide dismutase-like activity but demonstrates strong catalase-like activity (about 900 kU/mmol at 0.05-0.1 mmol/l concentration). The results indicate that at low concentration MMK exerts benign effect on cellular membrane that could find therapeutic usage. (author)

  8. Phytochemical profiles and antioxidant activity of 27 cultivars of tea.

    Science.gov (United States)

    Zeng, Liang; Luo, Liyong; Li, Hongjun; Liu, Ruihai

    2017-08-01

    Tea, rich in phytochemicals, has been suggested to have human health benefits. The phenolic profiles, antioxidant and antiproliferative activities of 27 tea cultivars were determined. Wide ranges of variation were found in analyzed cultivars for the contents of water-soluble phenolics (121.6-223.7 mg/g dry weight (DW)), total catechins (TC) (90.5-177.2 mg/g DW), antioxidant activities (PSC values 627.3-2332.3 μmol of vitamin C equiv/g DW, ORAC values (1865.1-3489.3 μmol of vitamin C equiv/g DW), CAA values (37.7-134.3 μmol of QE/g DW without PBS wash and 25.3-75.4 μmol of QE/g DW with PBS wash) and antiproliferative activity (53.0-90.8% at the concentration of 400 μg/mL extracts). The PSC, ORAC and CAA values were significantly correlated with phenolics, epicatechin gallate (ECG), CC and TC. Knowledge of specific differences among tea cultivars is important for breeding tea cultivars and gives sights to its potential application to promote health.

  9. Antioxidant activity of linalool in patients with carpal tunnel syndrome.

    Science.gov (United States)

    Seol, Geun-Hye; Kang, Purum; Lee, Hui Su; Seol, Geun Hee

    2016-02-02

    Carpal tunnel syndrome (CTS) is a common peripheral neuropathy and ischemic-reperfusion injury. Oxidative stress is considered a major cause of CTS. Linalool, a component of essential oils, has antioxidant activity. This study was designed to determine the effects of linalool inhalation on oxidative stress in patients with CTS. This double-blind, placebo-controlled study assessed the effects of linalool inhalation on oxidative stress in patients with CTS. Thirty-seven subjects, with and without CTS, were randomized to inhalation of 1% linalool or carrier oil. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, systolic blood pressure (sBP), diastolic blood pressure (dBP) and pulse rate were analyzed. DPPH inhibition was significantly higher in both experimental groups than in their respective controls. Moreover inhalation of linalool reduced sBP, dBP and pulse rate in the CTS group, and pulse rate in the non-CTS group. However, there were no significant differences among the study groups in nitrite levels, sBP, dBP and pulse rate. Inhalation of linalool increases antioxidative activity and reduces blood pressure and pulse rate in patients with CTS.

  10. Antioxidant Activity of a Red Lentil Extract and Its Fractions

    Science.gov (United States)

    Amarowicz, Ryszard; Estrella, Isabell; Hernández, Teresa; Dueñas, Montserrat; Troszyńska, Agnieszka; Agnieszka, Kosińska; Pegg, Ronald B.

    2009-01-01

    Phenolic compounds were extracted from red lentil seeds using 80% (v/v) aqueous acetone. The crude extract was applied to a Sephadex LH-20 column. Fraction 1, consisting of sugars and low-molecular-weight phenolics, was eluted from the column by ethanol. Fraction 2, consisting of tannins, was obtained using acetone-water (1:1; v/v) as the mobile phase. Phenolic compounds present in the crude extract and its fractions demonstrated antioxidant and antiradical activities as revealed from studies using a β-carotene-linoleate model system, the total antioxidant activity (TAA) method, the DPPH radical-scavenging activity assay, and a reducing power evaluation. Results of these assays showed the highest values when tannins (fraction 2) were tested. For instance, the TAA of the tannin fraction was 5.85 μmol Trolox® eq./mg, whereas the crude extract and fraction 1 showed 0.68 and 0.33 μmol Trolox® eq./mg, respectively. The content of total phenolics in fraction 2 was the highest (290 mg/g); the tannin content, determined using the vanillin method and expressed as absorbance units at 500 nm per 1 g, was 129. There were 24 compounds identified in the crude extract using an HPLC-ESI-MS method: quercetin diglycoside, catechin, digallate procyanidin, and p-hydroxybenzoic were the dominant phenolics in the extract. PMID:20054484

  11. Influence of sonication on the phenolic content and antioxidant activity of Terminalia catappa L. leaves.

    Science.gov (United States)

    Annegowda, H V; Anwar, L N; Mordi, M N; Ramanathan, S; Mansor, S M

    2010-11-01

    This study was designed to evaluate the phenolic content and antioxidant activity of ethanolic extracts from T. catappa leaves obtained by different intervals of sonication. Three commonly used methods were followed to evaluate phenolic content and four in vitro methods like 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay, ferric reducing antioxidant potency (FRAP), and total antioxidant capacity assays for measuring the antioxidant activities. Antioxidant values of these assays were expressed in terms of milligrams vitamin C equivalent (VCE) antioxidant activities. This study showed that extract obtained with 40 minutes of sonication possessed significant (P catappa leaf above 40 minutes was found to be unsuitable for extracting out phenolic contents. Even the results of antioxidant assays showed that 40 minutes of the sonicated extract exhibited significant (P catappa leaves.

  12. Ginsan activated the antioxidant defense systems in irradiated mice

    Energy Technology Data Exchange (ETDEWEB)

    Song, Jie Young; Son, Soo Jung; Ahn, Ji Yeon; Shim, Ji Young; Han, Young Soo; Jung, In Sung; Yun, Yeon Sook [KIRMS Daegu (Korea, Republic of)

    2003-07-01

    Ginsan, a polysaccharide extracted from Panax ginseng, has hematopoietic activity and is also known as a good biological-response modifier. In this investigation, we studied the effects of ginsan on the {gamma}-radiation induced alterations of some antioxidant systems in spleen of Balb/c mice. There are many data that irradiation induces Reactive Oxygen Species (ROS), which plays an important causative role in radiation damage of cell. The level of ROS in cells is regulated by enzymatic and nonenzymatic antioxidant systems. The most powerful ones among them are superoxide dismutases (SODs) catalyzing the dismutation of superoxide anion radical o{sub 2} to H{sub 2}O{sub 2}, catalase deactivating h-2O{sub 2} and reduced glutathion (GSH) detoxifying H{sub 2}O{sub 2} and other ROS> At the 5{sub th} day after sublethal whole body irradiation, splenocytes of irradiated mice expressed only marginally increased levels of Mn-SOD, however, Cu/Zn-SOD, catalase, thioredoxine reductase (TR) and thioredoxine (TRX) mRNA (135% increase compared to control), however, the combination of irradiation with ginsan increased the SODs and GPX production more effectively. In addition to the above results, we obtained the similar data of protein expression. The enzyme activities of SOD, catalase, and GPX of ginsan-treated and irradiated mice were significantly enhanced by 140, 115, 126% respectively, compared with those of irradiated mice. Based on these results, we propose that the induction of antioxidant enzymes of ginsan is at least in part due to its capacity to protect against radiation.

  13. TOTAL ANTIOXIDANT ACTIVITY OF SOME BASIDIOMYCETES STRAINS IN GROWTH DYNAMIC

    Directory of Open Access Journals (Sweden)

    O. V. Fedotov

    2016-08-01

    Full Text Available The work is devoted to the study of total antioxidant activity (AOA in the growth dynamics of basidiomycetes strains in their periodic surface cultivation on glucose-peptone medium. Subjects of research are mycelium and culture filtrate (CF from 57 strains, 5 of which are belong to 5 types of Polyporales order, and 52 of which are belong to the 7 types of Agaricales order. In order to study the dynamics of growth used method for determining the weight of absolutely dry biomass accumulation (ADB. Total AOA of mycological material was evaluated by inhibition of lipid peroxidation products accumulation intensity in the model oxidation reaction of Tween-80 by air oxygen. It was found that the most productive in terms of the accumulation of ADB are strains F. velutipes F-610 and P. eryngii P-er. Lowest values of ADB accumulation recorded for strains P. ostreatus P-14 and P-192 and P. citrinopileatus P sіtr. Were selected the most productive strains of Basidiomycetes for the level of total AOA in mycelium and CF. There are strains P. eryngii P-er, P. citrinopileatus P sіtr, P. ostreatus P-035, F. hepatica Fh-08, A. cylindracea 960, P. ostreatus P-081, P-082, P-087, P. citrinopileatus P sіtr. Has not been established the dependence between the growth and the antioxidant activity of the 9- and 12-day fungal cultures. Selected producers of natural antioxidants may be used as biological agents in biotechnology.

  14. Antioxidant and antimicrobial activities of squid ink powder

    Directory of Open Access Journals (Sweden)

    Fatimah Zaharah, M.Y.

    2017-10-01

    Full Text Available Economic development in Malaysia has led to increasing quantity and complexity of generated waste or by-product. The main objective of this study is to investigate the antioxidant and antimicrobial activities of squid ink powder. The squid ink was collected from fresh squid and dried using freeze dryer before it was ground into powder. The yield of squid ink was 22.82% after freeze-drying which was 69.37g in amount. Proximate composition analysis as well as two total antioxidant activity assays named 2,2-diphenyl-1-picrylhydrazyl (DPPH assay and Ferric Reducing Antioxidant Power (FRAP assay, and antimicrobial analysis were done on the powdered squid ink. The proximate results of squid ink powder were 4.43 ± 0.29% moisture, 62.46 ± 0.62% protein, 3.96 ± 0.08% fat, and 9.29 ± 0.05% ash. Results of DPPH assay showed that water extraction of squid ink powder has the highest 94.87 ± 4.87%, followed by ethanol 67.57 ± 7.55%, and hexane extract 2.10 ± 1.18%. FRAP assay result presented the same trend with water extraction had the highest value of 929.67 ± 2.31 μmol Fe (II / g of sample extract, followed by ethanol extract 201.00 ± 26.29 μmol Fe (II per gram sample and hexane 79.67 ± 12.66 μmol Fe (II / g of sample extract. Both water and ethanol extract showed antimicrobial properties with inhibition range of 7 to 15 mm, respectively. Fresh squid ink had 1.254 × 103 colony forming unit per gram of sample of microbial content. Squid ink powder had protein as major compound and microbial content was below from standard value of fisheries products as stated in Food Act 1983 and Regulation 1985.

  15. Antioxidant activity of Costa Rican propolis: a multivariate analysis approach

    International Nuclear Information System (INIS)

    Umana Rojas, Eduardo; Solado, Godofredo; Tamayo-Castillo, Giselle

    2013-01-01

    Propolis is produced by Apis mellifera bees from resins of plants that are found around the apiary. The chemical composition is highly variable and Costa Rica has reported without studies of characterization to define the types of propolis in the country. 119 samples were collected from beekeeping areas of the country. The spectrum of 1 H-NMR and its antioxidant activity against DPPH radical were measured. The spectra have been divided into 243 blocks of 0,04 ppm and processed with the Minitab software for multivariate analysis. 99 of the samples collected were used for construction of models for the valuation of the predictive ability of the model have been used coefficients of determination (R 2 ) of prediction by the software and the remaining 20 samples. The existence of three types of propolis with chemically different metabolomes were determined by principal component analysis (PCA). A prediction model was constructed by analysis of partial least squares (PLS). The prediction model has allowed to classify a propolis according to the level of antioxidant activity (AAO), high (type I and II) or low (type III) from the spectrum of 1 H-NMR. The R 2 has been 0.88 and R 2 prediction of 0, 718 for new samples. The nconiferyl benzoate of group I and nemorosone of the group II as two discriminated antioxidants among the groups I and II were isolated and high concentration levels of these compounds have been differentiated with respect to type III. This has allowed the construction of a linear discriminant model with a success rate of 100% for the samples used for formulation and 92,9 for the prediction of different samples. The classification systems could be applied to the standardization of the quality of propolis from Costa Rica for future medicinal or cosmetic applications that take advantage of its antioxidant properties. Also, the methylated derivative has isolated and identified of the nconiferyl benzoate thereof propolis than was obtained his counterpart

  16. Composition and antioxidant activity of Trigona carbonaria honey from Australia.

    Science.gov (United States)

    Oddo, Livia Persano; Heard, Tim A; Rodríguez-Malaver, Antonio; Pérez, Rosa Ana; Fernández-Muiño, Miguel; Sancho, María Teresa; Sesta, Giulio; Lusco, Lorenzo; Vit, Patricia

    2008-12-01

    Stingless bees (Tribe Meliponini) are a diverse group of highly eusocial bees distributed throughout the tropics and subtropics. Trigona carbonaria honey, from Australia, was characterized by traditional physicochemical parameters (acidity, sugars, diastase, electrical conductivity, hydroxymethylfurfural, invertase, nitrogen, and water content) and other compositional factors (flavonoids, polyphenols, organic acids, and water activity), as well as total antioxidant capacity and radical scavenging activity. For the Australian T. carbonaria, the traditional analytical parameters were similar to those previously reported for neotropical stingless bee honey and confirm that honeys produced by Meliponini bees possess several physicochemical properties that are distinctly different from Apis mellifera honey, with higher values of moisture (26.5 +/- 0.8 g of water/100 g of honey), water activity (0.74 +/- 0.01), electrical conductivity (1.64 +/- 0.12 mS/cm), and free acidity (124.2 +/- 22.9 mEq/kg of honey) and a very low diastase activity (0.4 +/- 0.5 diastase number) and invertase activity (5.7 +/- 1.5 invertase number). The sugar spectrum was quite different from that of A. mellifera honey, with 20.3 +/- 2.9 g of maltose/100 g of honey. The values of pH (4.0 +/- 0.1), lactonic acidity (4.7 +/- 0.8 mEq/kg of honey), sucrose (1.8 +/- 0.4 g/100 g of honey), and fructose/glucose ratio (1.42 +/- 0.13) fell in the same ranges as those of A. mellifera honey. Citric (0.23 +/- 0.09) and malic (0.12 +/- 0.03) acid concentrations (in g/kg of honey) of T. carbonaria honeys were in the range described for A. mellifera honey. D-Gluconic was more concentrated (9.9 +/- 1.3 g/kg of honey), in the range of Italian Castanea, Thymus, Arbutus, and honeydew honeys. Flavonoid content was 10.02 +/- 1.59 mg of quercetin equivalents/100 g of honey, and polyphenol contents were 55.74 +/- 6.11 mg of gallic acid equivalents/100 g of honey. The antioxidant activity, expressed as percentage of 2

  17. Antioxidant and nitric oxide synthase activation properties of Ganoderma applanatum.

    Science.gov (United States)

    Acharya, Krishnendu; Yonzone, Parinita; Rai, Manjula; Rupa, Acharya

    2005-10-01

    In vitro evaluation of antioxidant activities of Ganoderma applanatum showed significant inhibition of lipid peroxidation, and potent hydroxyl radical scavenging activity when compared with standard drug catechin. IC50 values of crude, boiled and ethanolic extracts of G. applanatum were 604.8, 624 and 267 microg/ml, respectively in case of hydroxyl radical scavenging activity, and 441, 520.5 and 166.16 microg/ml, respectively in case of lipid peroxidation. Furthermore, crude, boiled and ethanolic extracts also increased significantly nitric oxide production (156.67, 121.88 and 742 pmole/mg dry wt/hr, respectively) over the control. The results of present investigation revealed that G. applanatum have potential therapeutic use.

  18. Antioxidant and cytotoxic activities of 'acai' (Euterpe precatoria Mart.)

    International Nuclear Information System (INIS)

    Galotta, Ana Lucia Q.A.; Boaventura, Maria Amelia D.; Lima, Luciana A. R.S.

    2008-01-01

    Decoction of roots of the Amazonian palm acai (Euterpe precatoria Mart.) is widely used by Brazilian and Peruvian people as an anti-inflammatory, to heal kidney and liver diseases and against snake bites. In this study, the antioxidant activity of extracts and flavonoids (quercetin, catechin, epicatechin, rutin and astilbin) isolated from roots and leaf stalks of E. precatoria was investigated using β-carotene in TLC plates and DPPH radical scavenging in a spectrophotometric bioassay. All extracts and flavonoids showed activity. Also, the cytotoxic activity of these extracts was evaluated by the brine shrimp (Artemia salina) larvicide bioassay and was lower than that of lapachol, used as control. The presence of flavonoids and sitosterol-3-O-β-D-glucopyranoside in the extracts can justify the use of the plant in traditional medicine. (author)

  19. Antioxidant Activity in Two Pearl Millet (Pennisetum typhoideum Cultivars as Influenced by Processing

    Directory of Open Access Journals (Sweden)

    Florence Suma Pushparaj

    2014-02-01

    Full Text Available Research on the effect of processing on the retention of bioactive components with potential antioxidant activity is gaining importance. The objective of this investigation was to evaluate the effect of various processing methods (milling, boiling, pressure cooking, roasting and germination respectively on the antioxidant components as well as the antioxidant activities in the commonly used pearl millet cultivars—Kalukombu (K and Maharashtra Rabi Bajra (MRB. The methanolic extracts of processed pearl millet flours were analyzed for 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical scavenging activity, reducing power assay (RPA and ferric reducing antioxidant power (FRAP assays respectively. The samples were also evaluated for tannin, phytic acid and flavonoid content which was then correlated with the antioxidant activity assayed using three methods. The results indicated that the bran rich fraction showed high antioxidant activity (RPA owing to high tannin, phytic acid and flavonoid levels. Heat treatments exhibited significantly (P ≤ 0.05 higher antioxidant activity (DPPH scavenging activity and RPA reflecting the high flavonoid content. Processing did not have any significant effect on the FRAP activity of pearl millet. The data on the correlation coefficient suggests that DPPH radical scavenging activity and reducing power assay in the K variety was largely due to the presence of flavonoid content, however in MRB, no relationship was found between antioxidant activities and antioxidant components.

  20. Evaluation of antioxidant activity of leafy vegetables and beans with myoglobin method.

    Science.gov (United States)

    Terashima, Masaaki; Fukukita, Akiko; Kodama, Riho; Miki, Haruka; Suzuki, Mayuko; Ikegami, Maya; Tamura, Noriko; Yasuda, Akari; Morikawa, Mami; Matsumura, Saki

    2013-03-01

    KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. Antioxidant activity of seven leafy vegetables and four beans against peroxyl radical, hydroxyl radical, hypochlorite ion, and peroxynitrite ion has been measured using myoglobin as a reporter probe (myoglobin method). Conventional DPPH method was also used to evaluate antioxidant activity of the samples. Difference of activity against different reactive oxygen species (ROS) and reactive nitrogen species (RNS) was characterized by plotting the data in a 5-axe cobweb chart. This plot clearly showed the characteristics of the antioxidant activity of the leafy vegetables and the beans. The samples examined in this work were categorized into four groups. (1) The samples showed high antioxidant activity against all ROS and RNS: daikon sprout, spinach, Qing-geng-cai, and onion. (2) The samples showed high antioxidant activity against peroxyl radical: red bean and soy bean. (3) The samples showed high antioxidant against hypochlorite ion: broccoli floret, cabbage, and Chinese cabbage. (4) The samples showed weak antioxidant activity against all ROS and RNS: cowpea and common beans. Our protocol is probably useful to characterize antioxidant activity of the crops of different cultivars, the crops obtained in different growing environments and growing seasons, the crops harvested at different age, and the crops stored in the different conditions, as well as the changes of activity during cooking process of the crops.