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Sample records for strawberry fruit development

  1. Structural changes in cell wall pectins during strawberry fruit development.

    Science.gov (United States)

    Paniagua, Candelas; Santiago-Doménech, Nieves; Kirby, Andrew R; Gunning, A Patrick; Morris, Victor J; Quesada, Miguel A; Matas, Antonio J; Mercado, José A

    2017-09-01

    Strawberry (Fragaria × anannasa Duch.) is one of the most important soft fruit. Rapid loss of firmness occurs during the ripening process, resulting in a short shelf life and high economic losses. To get insight into the role of pectin matrix in the softening process, cell walls from strawberry fruit at two developmental stages, unripe-green and ripe-red, were extracted and sequentially fractionated with different solvents to obtain fractions enriched in a specific component. The yield of cell wall material as well as the per fresh weight contents of the different fractions decreased in ripe fruit. The largest reduction was observed in the pectic fractions extracted with a chelating agent (trans-1,2- diaminocyclohexane-N,N,N'N'-tetraacetic acid, CDTA fraction) and those covalently bound to the wall (extracted with Na 2 CO 3 ). Uronic acid content of these two fractions also decreased significantly during ripening, but the amount of soluble pectins extracted with phenol:acetic acid:water (PAW) and water increased in ripe fruit. Fourier transform infrared spectroscopy of the different fractions showed that the degree of esterification decreased in CDTA pectins but increased in soluble fractions at ripen stage. The chromatographic analysis of pectin fractions by gel filtration revealed that CDTA, water and, mainly PAW polyuronides were depolymerised in ripe fruit. By contrast, the size of Na 2 CO 3 pectins was not modified. The nanostructural characteristics of CDTA and Na 2 CO 3 pectins were analysed by atomic force microscopy (AFM). Isolated pectic chains present in the CDTA fractions were significantly longer and more branched in samples from green fruit than those from red fruit. No differences in contour length were observed in Na 2 CO 3 strands between samples of both stages. However, the percentage of branched chains decreased from 19.7% in unripe samples to 3.4% in ripe fruit. The number of pectin aggregates was higher in green fruit samples of both

  2. Quality development of Danish strawberries

    DEFF Research Database (Denmark)

    Østergaard, Peder; Stacey, Julia

    1999-01-01

    The quality of fruit and berries has often been the topic of debate and Danish strawberries have been both praised and criticized. Therefore the Danish Institute of Agricultural Sciences, Danish Fruit Growers' Association, the Danish Veterinary and Food Administration and MAPP have joined together...... in a project about quality assurance of strawberries. One purpose of the project has been to provide systematic knowledge about Danish consumers' attitudes towards strawberries, knowledge, buying behaviour and consumption of strawberries. Another purpose has been to take an active part in the development...... of new strawberry varieties and growing methods and also to support better distribution and sale of strawberries. During 1999, focus group interviews and a representative study with 1174 householdswere carried out. In-home tests of existing and new varieties of strawberries will be carried out during...

  3. Development of Anastrepha fraterculus (Diptera: Tephritidae) Related to the Phenology of Blueberry, Blackberry, Strawberry Guava, and Surinam Cherry Fruits.

    Science.gov (United States)

    Bisognin, M; Nava, D E; Diez-Rodríguez, G I; Valgas, R A; Garcia, M S; Krolow, A C R; Antunes, L E C

    2015-02-01

    Anastrepha fraterculus (Wiedemann, 1830) is the main pest of temperate climate orcharding. The study investigated the development of A. fraterculus related to phenological stage of blueberry, blackberry, strawberry guava, and Surinam cherry trees. The phenological stages I (green fruits), II (intermediate ripening stage of fruits), and III (fruits close to harvesting) were determined, and they are from 8th, 10th, and 11th week; 6th, 8th, and 9th week; 8th, 13th, and 16th week; and 5th, 6th, and 7th week after the first flowering of blueberry, blackberry, strawberry guava, and Surinam cherry trees, respectively. We collected fruits from orchards to determine the infestation index using the formula: number of pupa/fruit weight. To investigate the development of A. fraterculus, we determined the following biological parameters: egg-to-adult period, weight of pupae, oviposition period, fecundity, number of pupae, and number of infested fruits. The infestation index for the fruits collected in the field was greater in strawberry guava and Surinam cherry fruits. In the laboratory, the development of A. fraterculus occurred in stage III of blueberry. In blackberry, besides stage III, we also observed the development in stage II, however, at lower infestation. In strawberry guava, the development of A. fraterulus occurred in stages II and III, and the development in both stages was similar. For Surinam cherry, the development occurred in the three phenological stages with similar values for biological parameters. Overall, of the four hosts studied, the strawberry guava and Surinam cherry fruits allowed a better biological development of A. fraterculus, corroborating its preference for fruits native to Brazil. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  4. Storage of irradiated strawberry fruits

    International Nuclear Information System (INIS)

    Popova, L.

    1977-01-01

    Pocahontas strawberries both of green house and field production have been stored at 3 deg C for 10 and 12 days, respectively, after treatment with 100000, 200000 and 300000 Roe in comparison with unirradiated fruits. No explicit correlation was observed regarding the keeping qualities of fruits, their chemical composition (dry matter, sugars, acids and vitamin C) when stored after a different gamma-ray irradiation. (S.P.)

  5. Fruit Detachment and Classification Method for Strawberry Harvesting Robot

    Directory of Open Access Journals (Sweden)

    Guo Feng

    2008-11-01

    Full Text Available Fruit detachment and on-line classification is important for the development of harvesting robot. With the specific requriements of robot used for harvesting strawberries growing on the ground, a fruit detachment and classification method is introduced in this paper. OHTA color spaces based image segmentation algorithm is utilized to extract strawberry from background; Principal inertia axis of binary strawberry blob is calculated to give the pose information of fruit. Strawberry is picked selectively according to its ripeness and classified according to its shape feature. Histogram matching based method for fruit shape judgment is introduced firstly. Experiment results show that this method can achieve 93% accuracy of strawberry's stem detection, 90% above accuracy of ripeness and shape quality judgment on black and white background. With the improvement of harvesting mechanism design, this method has application potential in the field operation.

  6. Fruit Detachment and Classification Method for Strawberry Harvesting Robot

    Directory of Open Access Journals (Sweden)

    Guo Feng

    2008-03-01

    Full Text Available Fruit detachment and on-line classification is important for the development of harvesting robot. With the specific requriements of robot used for harvesting strawberries growing on the ground, a fruit detachment and classification method is introduced in this paper. OHTA color spaces based image segmentation algorithm is utilized to extract strawberry from background; Principal inertia axis of binary strawberry blob is calculated to give the pose information of fruit. Strawberry is picked selectively according to its ripeness and classified according to its shape feature. Histogram matching based method for fruit shape judgment is introduced firstly. Experiment results show that this method can achieve 93% accuracy of strawberry's stem detection, 90% above accuracy of ripeness and shape quality judgment on black and white background. With the improvement of harvesting mechanism design, this method has application potential in the field operation.

  7. Radiation preservation of strawberry fruit: a review

    International Nuclear Information System (INIS)

    Quaranta, H.O.; Piccini, J.L.

    1984-01-01

    This paper reviews the up-to-date literature on strawberry fruit preservation by irradiation, including microbiological and nutritional aspects, as well as chemical alterations in its components through analysis of vitamin content, carbohydrates, amino acids and fatty acids present, estimation of content of volatiles, and influence of irradiation on shelf-life and appearance. (author)

  8. Effect of mulching systems on fruit quality and phytochemical composition of newly developed strawberry lines

    Directory of Open Access Journals (Sweden)

    Li Fan

    2012-06-01

    Full Text Available The effects of three mulching systems on total yield, average yield per plant, average fruit weight, soluble solids content (SSC, titratable acidity (TA, firmness and oxygen radical absorbance capacity (ORAC of two newly developed lines (‘Orléans’ and ‘Saint Pierre’, one advanced selection (‘SJ8976-1’ and two commonly used cultivars (‘Jewel’ and ‘Kent’ were evaluated. The studied mulching systems were: plastic mulch (PM, mulch with row cover (PMRC, and matted-row system (MRS. Results showed that plastic mulch with row cover (PMRC generally increased yield per plant, average fruit weight, SSC, firmness and ORAC, but differences varied within harvest times. No significant differences in total yield and TA were observed under the selected mulching systems. Both PMRC and PM accelerated the harvest periods compared to MRS. ‘Kent’ and ‘Jewel’ had the highest total yield while ‘SJ8976-1’ and ‘St-Pierre’ had the highest average fruit weight. The highest SSC, TA and ORAC were found in ‘Jewel’. There was no interaction between the mulching systems and genotypes, indicating that the effect of production system is independent of cultivars. PMRC seems to be a better growing system, improving fruit quality and increasing the nutritional value of all genotypes. By allowing off-season fruit production in cool climates, PMRC can be an alternative method to the costly high tunnels.

  9. Over-expression of Gene FaASR Promotes Strawberry Fruit Coloring

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    Liu Zhongjie

    2015-11-01

    Full Text Available Fruit development and ripening is a complicate process. Although much progress has been made on the ripenig process, the molecular mechamism of fruit development is not yet clear. In this study, we used ‘Sweet Charlie’ strawberry as test materials, based on cloning the strawberries ASR homologous gene, we carried out the bioinformatics and temporal expression analysis of FaASR, by manipulating ASR gene expression level in strawberry fruit, we tested the changes of physiological indicators, including sugar, ABA, pigments content, and fruit firmness, as well as phenotypic changes. In addition, we measured the expression changes of some anthocyanin-related gene, such as CHS and UFGT, by which we revealed the regulation mechanisms of ASR gene over strawberry fruit ripening. Strawberry ASR contained a typical domain of ABA/WDS that was related to fruit ripening and stress-resistance, and ASR gene over-expression could promote strawberry fruit coloring, endogenous ABA and sucrose accumulation, fruit softening, and induced the transcription levels of anthocyanin-related genes CHS and UFGT. The present study will further reveal the molecular mechanisms of information transmission in fruit development, and will also play an important foundation for future molecular improvement of strawberries breeding.

  10. Evaluation of quality parameters of strawberry fruits in modified ...

    African Journals Online (AJOL)

    The objective of the present study is to evaluate the effect of modified atmosphere packaging (MAP) during storage on some quality parameters of strawberries. Strawberries (cv. Camarosa) were harvested when mature, forced air cooled and divided into two groups as fruits in MAP and control. After packaging, fruits were ...

  11. Strawberry Anthracnose Ripe Fruit Rot and Management Recommendations

    OpenAIRE

    Louws, Frank

    2009-01-01

    Briefly describes anthracnose symptoms on ripe strawberry fruit, then recommends ways to manage for the anthracnose, including removal and burying or burning infected and nearby plants, avoiding overhead irrigation, and picking fruits from the infected areas last.

  12. Controlled atmosphere storage of wild strawberry fruit (Fragaria vesca L.).

    Science.gov (United States)

    Almenar, Eva; Hernández-Muñoz, Pilar; Lagarón, José M; Catalá, Ramón; Gavara, Rafael

    2006-01-11

    Controlled atmosphere storage technology to extend the shelf life of "Reina de los Valles" wild strawberry fruit (Fragaria vesca L.) was studied. Fruits were stored at 3 degrees C for three weeks in different atmosphere compositions: 0.05% CO2/21% O2 (air), 3% CO2/18% O2, 6% CO2/15% O2, 10% CO2/11% O2, and 15% CO2/6% O2. The effect of gas composition on soluble solids content, titrable acidity, pH, off-flavor, aroma volatiles, and consumer preference was monitored. The result showed that the 10% CO2/11% O2 combination can efficiently prolong the shelf life of wild strawberries by maintaining the quality parameters within acceptable values, through inhibiting the development of Botrytis cinerea, without significantly modifying consumer acceptance.

  13. Active package for wild strawberry fruit (Fragaria vesca L.).

    Science.gov (United States)

    Almenar, Eva; Del Valle, Valeria; Catala, Ramón; Gavara, Rafael

    2007-03-21

    An antimicrobial active package has been developed to improve the safety and quality of wild strawberries, as well as extending their shelf life. The fruits were packed in equilibrium-modified atmosphere packaging (EMAP), and the effect on Botrytis cinerea growth and on the quality parameters of the fruit by the addition of different amounts of 2-nonanone, an antifungal volatile compound naturally present in strawberries, was investigated during storage at 10 and 22 degrees C. The temperature of 10 degrees C was chosen as the temperature used at points of sale, and 22 degrees C was chosen as the control temperature. Fungal growth was inhibited in all cases, possibly due to the synergistic effect of high CO2 partial pressures and the presence of the antifungal compound. Weight, soluble solids, titrable acidity, and anthocyanin losses were retarded by the presence of 2-nonanone. This effect was more pronounced as the 2-nonanone concentration was increased at both temperatures. Therefore, an active package that releases 2-nonanone inhibits fungal decay and delays the senescence of highly perishable wild strawberry fruit.

  14. FaPOD27 functions in the metabolism of polyphenols in strawberry fruit (Fragaria sp.

    Directory of Open Access Journals (Sweden)

    Su-Ying eYeh

    2014-10-01

    Full Text Available The strawberry (Fragaria × ananassa is one of the most preferred fresh fruit worldwide, accumulates numerous flavonoids but has limited shelf life due to excessive tissue softening caused by cell wall degradation. Since lignin is one of the polymers that strengthen plant cell walls and might contribute to some extent to fruit firmness monolignol biosynthesis was studied in strawberry fruit. Cinnamoyl-CoA reductase (CCR, cinnamyl alcohol dehydrogenase (CAD, and a peroxidase (POD27 gene were strongly expressed in red, ripe fruit whereas a second POD gene was primarily expressed in green, immature fruit. Moreover, FaPOD27 transcripts were strongly and constitutively induced in fruits exposed to Agrobacterium infection. Gene expression levels and enzymatic activities of FaCCR and FaCAD were efficiently suppressed through RNAi in FaCCR- and FaCAD-silenced strawberries. Besides, significantly elevated FaPOD transcript levels were detected after agroinfiltration of pBI-FaPOD constructs in fruits. At the same time, levels of G-monomers were considerably reduced in FaCCR-silenced fruits whereas the proportion of both G- and S-monomers decisively decreased in FaCAD-silenced and pBI-FaPOD fruits. Development, firmness, and lignin level of the treated fruits were similar to pBI-intron control fruits, presumably attributed to increased expression levels of FaPOD27 upon agroinfiltration. Additionally, enhanced firmness, accompanied with elevated lignin levels, was revealed in chalcone synthase-deficient fruits (CHS-, independent of down- or up-regulation of individual and combined FaCCR, FaCAD, and FaPOD genes by agroinfiltration, when compared to CHS-/pBI-intron control fruits. These approaches provide further insight into the genetic control of flavonoid and lignin synthesis in strawberries. The results suggest that FaPOD27 is a key gene for lignin biosynthesis in strawberry fruit and thus to improving the firmness of strawberries.

  15. Colored plastic mulch microclimates affect strawberry fruit yield and quality.

    Science.gov (United States)

    Shiukhy, Saeid; Raeini-Sarjaz, Mahmoud; Chalavi, Vida

    2015-08-01

    Significant reduction of strawberry (Fragaria × ananassa, Duch.) fruit yield and quality, as a consequence of conventional cultivation method, is common in the Caspian Sea region, Iran. Recently, growers started using plastic mulches to overcome these shortcomings. Plastic mulches have different thermal and radiation properties and could affect strawberry fruit yield and quality. In the present study, the effect of different colored plastic mulches (black, red, and white) along with conventional practice was tested on yield and quality of strawberry Camarosa cultivar, in a completely randomized block design. Colored plastic mulches had highly significant effect on fruit weight, size, and phytochemical contents. In the most harvest times, mean fruit weight was significantly higher in red plastic relative to white and control treatments. Total fruit weight of plastic mulches was not significantly different, while all were statistically higher than that of control. Fruit size significantly increased over red plastic mulch. Total fruit numbers over plastic mulches were significantly higher than that of control treatment. The content of phenolic compounds was similar between treatments, while anthocyanin content, IC(50) value, and flavonoid content significantly were affected by colored plastics. In conclusion, colored plastic mulches could affect strawberry fruit weight and quality through altering strawberry thermal and radiation environment.

  16. Transient transformation meets gene function discovery: the strawberry fruit case

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    Michela eGuidarelli

    2015-06-01

    Full Text Available Beside the well known nutritional and health benefits, strawberry (Fragaria X ananassa crop draws increasing attention as plant model system for the Rosaceae family, due to the short generation time, the rapid in vitro regeneration, and to the availability of the genome sequence of F. X ananassa and of the closely related F. vesca species. In the last years, the use of high-throughput sequence technologies provided large amounts of molecular information on the genes possibly related to several biological processes of this crop. Nevertheless, the function of most genes or gene products is still poorly understood and needs investigation. Transient transformation technology provides a powerful tool to study gene function in vivo, avoiding difficult drawbacks that typically affect the stable transformation protocols, such as transformation efficiency, transformants selection and regeneration. In this review we provide an overview of the use of transient expression in the investigation of the function of genes important for strawberry fruit development, defence and nutritional properties. The technical aspects related to an efficient use of this technique are described, and the possible impact and application in strawberry crop improvement are discussed.

  17. Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin.

    Science.gov (United States)

    Ishibashi, Misaki; Yoshikawa, Hiroki; Uno, Yuichi

    2017-06-02

    Strawberry fruit contain the allergenic Fra a proteins, members of the pathogenesis-related 10 protein family that causes oral allergic syndrome symptoms. Fra a proteins are involved in the flavonoid biosynthesis pathway, which might be important for color development in fruits. Auxin is an important plant hormone in strawberry fruit that controls fruit fleshiness and ripening. In this study, we treated strawberry fruits with exogenous auxin or auxin inhibitors at pre- and post-harvest stages, and analyzed Fra a transcriptional and translational expression levels during fruit development by real-time PCR and immunoblotting. Pre-harvest treatment with 1-naphthaleneacetic acid (NAA) alone did not affect Fra a expression, but applied in conjunction with achene removal NAA promoted fruit pigmentation and Fra a protein accumulation. The response was developmental stage-specific: Fra a 1 was highly expressed in immature fruit, whereas Fra a 2 was expressed in young to ripe fruit. In post-harvest treatments, auxin did not contribute to Fra a induction. Auxin inhibitors delayed fruit ripening; as a result, they seemed to influence Fra a 1 expression. Thus, Fra a expression was not directly regulated by auxin, but might be associated with the ripening process and/or external factors in a paralog-specific manner.

  18. Application of multispectral imaging to determine quality attributes and ripeness stage in strawberry fruit.

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    Changhong Liu

    Full Text Available Multispectral imaging with 19 wavelengths in the range of 405-970 nm has been evaluated for nondestructive determination of firmness, total soluble solids (TSS content and ripeness stage in strawberry fruit. Several analysis approaches, including partial least squares (PLS, support vector machine (SVM and back propagation neural network (BPNN, were applied to develop theoretical models for predicting the firmness and TSS of intact strawberry fruit. Compared with PLS and SVM, BPNN considerably improved the performance of multispectral imaging for predicting firmness and total soluble solids content with the correlation coefficient (r of 0.94 and 0.83, SEP of 0.375 and 0.573, and bias of 0.035 and 0.056, respectively. Subsequently, the ability of multispectral imaging technology to classify fruit based on ripeness stage was tested using SVM and principal component analysis-back propagation neural network (PCA-BPNN models. The higher classification accuracy of 100% was achieved using SVM model. Moreover, the results of all these models demonstrated that the VIS parts of the spectra were the main contributor to the determination of firmness, TSS content estimation and classification of ripeness stage in strawberry fruit. These results suggest that multispectral imaging, together with suitable analysis model, is a promising technology for rapid estimation of quality attributes and classification of ripeness stage in strawberry fruit.

  19. Influence of silicon on production and fruit quality of strawberry

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    Maria Ligia Souza Silva

    2013-12-01

    Full Text Available In Brazil, the culture of strawberries is an important activity for small and medium farmers. Among the important factors for culture is fertilization, which can interfere in production and fruit quality. Silicon (Si is one of the little studied in fertilization, which demonstrated some beneficial to several different cultures. The objective of this study was to evaluate the influence of Si on production and concentration of anthocyanin and titratable acid in the fruit and reading SPAD (chlorophyll in the leaves of the strawberry. The experiment was conducted in a greenhouse using pots containing 14.5 kg of limed soil, fertilized and three plants (cultivar Milsei Tudla were transplanted to pots. Treatments consisted of application of Si at 0, 12.5, 25, 50 and 100 mg kg-1 rates, via soil or foliar. During this experiment the reading of SPAD (chlorophyll and the end of the experiment, the shoots was collected, dried, weighed, ground and analyzed by content and accumulation Si. The fruits were weighed and analyzed the concentration of anthocyanin, titratable acidity and Si concentration. The fertilization with Si, via soil or foliar applied, contributed to the increase in fruit production, especially with the application via soil. Applying foliar or soil promoted an increase in the values of titratable acidity and anthocyanins. Rates of Si via soil or foliar applied influenced the reading SPAD (chlorophyll, in the leaves. The Si independent of the form of application promoted improvements in strawberry crop.

  20. Classification of Strawberry Fruit Shape by Machine Learning

    Science.gov (United States)

    Ishikawa, T.; Hayashi, A.; Nagamatsu, S.; Kyutoku, Y.; Dan, I.; Wada, T.; Oku, K.; Saeki, Y.; Uto, T.; Tanabata, T.; Isobe, S.; Kochi, N.

    2018-05-01

    Shape is one of the most important traits of agricultural products due to its relationships with the quality, quantity, and value of the products. For strawberries, the nine types of fruit shape were defined and classified by humans based on the sampler patterns of the nine types. In this study, we tested the classification of strawberry shapes by machine learning in order to increase the accuracy of the classification, and we introduce the concept of computerization into this field. Four types of descriptors were extracted from the digital images of strawberries: (1) the Measured Values (MVs) including the length of the contour line, the area, the fruit length and width, and the fruit width/length ratio; (2) the Ellipse Similarity Index (ESI); (3) Elliptic Fourier Descriptors (EFDs), and (4) Chain Code Subtraction (CCS). We used these descriptors for the classification test along with the random forest approach, and eight of the nine shape types were classified with combinations of MVs + CCS + EFDs. CCS is a descriptor that adds human knowledge to the chain codes, and it showed higher robustness in classification than the other descriptors. Our results suggest machine learning's high ability to classify fruit shapes accurately. We will attempt to increase the classification accuracy and apply the machine learning methods to other plant species.

  1. Role of Brassinosteroid on Qualitative Characteristics Improvement of Strawberry Fruit cv. Paros

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    soheila mohammadrezakhani

    2017-02-01

    Full Text Available Introduction: Recently, strawberry growers have been mostly interested in growing cultivars for the fresh market because of its profitability, but on the other hand it requires more complicated technologies and well-educated workers. High quality of the fruit for the fresh market is an important factor attracts customers and determines their choice and prices. Fruit production cost for the fresh market needs to be calculated and efficient methods and technologies also should be taken into consideration. New environmentally friendly mineral-organic fertilizers can improve fruit quality and yield of dessert strawberry cultivars. The desired effects was obtained through the activity of fertilizer’s components, which very often belong to different groups of natural hormones, elicitors, vitamins, flavonoids, amino acids, etc. Numerous breeding programs have been aimed at improving strawberry taste and disease resistance. Three major components of fruit organoleptic quality are flavor, sweetness, and acidity. Several studies have been devoted to strawberry aroma. Fruit with intense flavor also have high titratable acidity and high soluble solids. Numerous studies have addressed strawberry sweetness and acidity. Fruit soluble solids, sugars, titratable acidity, and organic acids at maturity are quantitatively inherited. Moreover, there appears to be genetic variations for these fruit quality traits. Numerous biochemical changes are observed during strawberry development and especially during fruit ripening. The major soluble constituents of maturing and ripe strawberries are soluble sugars and organic acids. The major soluble sugars in strawberries are glucose, fructose, and sucrose. The major organic acid is citric acid. This acid contributes greatly to fruit titratable acidity, which declines gradually during fruit development. The sugar/ organic acid ratio is a major parameter of strawberry taste. Brassinosteroids (BRs are a class of poly hydroxyl

  2. Evaluation and public acceptance of irradiated strawberries and citrus fruits

    International Nuclear Information System (INIS)

    Del Mastro, Nelida L.; Kikuchi, Olivia K.; Villavicencio, Anna L.C.H.; Sabato, Susy F.; Rela, Paulo R.; Taipina, Magda S.; Mattiolo-Marchse, Sandra R.

    1999-01-01

    Irradiation is a physical process that can be applied to food in order to eliminate microorganisms, insects and other plagues as well as delay ripening or spoilage, thus lengthening its shelf life. In Brazil, the technique is only starting to be applied and is restricted to a few tons of dry or dehydrated food ingredients per year. Strawberry (Fragaria sp.) and citrus are usually attacked by various plagues. Both strawberry and citrus are included in the Brazilian legislation for irradiated foods. This work describes the first sensory trials of 2 varieties of strawberries and 3 varieties of citrus irradiated at IPEN. Irradiation was performed in a panoramic Co-60 source with doses ranging between 1.7 and 4 kGy. Untrained groups of panelists judged the quality of irradiated and non-irradiated control fruits. From the analysis of the results, it was concluded that there no significant differences in the characteristical properties of the fruits when they were irradiated with the doses recommended by the legislation. (author)

  3. Radiation processing of fruits: application to strawberries and prunes

    International Nuclear Information System (INIS)

    Levillain, M.

    1986-10-01

    Extending the shelf-life of fresh fruit by means of low-dose irradiation (radurization) is not a new idea: experiments in that field started in the early sixties. These experiments have actually proved that, in some cases, irradiation can achieve shelf-life extension, either through a delay in ripening (bananas), or through rot inhibition (soft cherries, apricots, tomatoes, strawberries). Alas, they have also highlighted the intolerance showed by a number of fruits when radurized: irradiation is apt to have them ripen more rapidly (peaches, nectarines) or to soften them too much (pears, table grapes, oranges, apples, plums, grapefruit, melons, honeydew melons). Even in those cases where irradiation results in a benefit, this benefit varies depending on the variety of fruit involved, as can be seen from a deep survey of the irradiation of strawberries. Preservation of dehydrated fruit is a different matter. Prunes, for instance, would be contamined by molds, wasn't it for the addition of sorbic acid during the fabrication process. Ionization of prunes can allow producers to avoid the use of a chemical, and to keep the prunes at a greater degree of humidity [fr

  4. Carotenoid composition of strawberry tree (Arbutus unedo L.) fruits.

    Science.gov (United States)

    Delgado-Pelayo, Raúl; Gallardo-Guerrero, Lourdes; Hornero-Méndez, Dámaso

    2016-05-15

    The carotenoid composition of strawberry tree (Arbutus unedo) fruits has been characterised in detail and quantified for the first time. According to the total carotenoid content (over 340 μg/g dw), mature strawberry tree berries can be classified as fruits with very high carotenoid content (>20 μg/g dw). (all-E)-Violaxanthin and 9Z-violaxanthin were found to be the major carotenoid pigments, accounting for more than 60%, responsible for the bright colour of the flesh of ripe fruits. In addition other 5,6-epoxide carotenoids, such as (all-E)-neoxanthin, (9'Z)-neoxanthin (all-E)-antheraxanthin and lutein 5,6-epoxide, together with (all-E)-lutein, (all-E)-zeaxanthin and (all-E)-β-carotene were found at high levels (>5-20 μg/g dw). The LC-MS (APCI+) analysis of the xanthophyll fraction in their native state (direct extract) revealed that most of them (>90%) were totally esterified with saturated fatty acids (capric, lauric, myristic, palmitic and stearic). Monoesters, homodiesters and heterodiesters of (all-E)-violaxanthin and 9Z-violaxanthin were the major pigments. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Mycorrhizal inoculation affects the phytochemical content in strawberry fruits

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    Ana Paula Cecatto

    2016-04-01

    Full Text Available The aim of this research was to evaluate the effect of the inoculation date of arbuscular mycorrhizal fungi on the fruit quality and the content of phytochemicals in a strawberry soilless growing system. The experiment was performed in Huelva (Spain and was conducted in a greenhouse on the La Rábida Campus of Huelva University under natural light and temperature from October 2013 to June 2014. Three short-day strawberry cultivars (‘Splendor’, ‘Sabrina’ and ‘Fortuna’ were grown in polyethylene bags filled with coconut fibres. Randomized block design, with 3 repetitions and factorial arrangement (3 cultivars x 3 treatments, was established. Each replicate consisted of one bag with 12 plants supporting structures at 40 cm height. The treatments were: T1 = mycorrhizal inoculation in the transplantation; T2 = mycorrhizal inoculation 30 days after transplantation (DAT; and T0 = control treatment, without inoculation. Arbuscular mycorrhizal fungi inoculation significantly affected the contents of anthocyanin and phenolics. When the inoculation is performed in the transplantation, the fruits showed a high content of anthocyanin and total phenolics. The mycorrhizal inoculation influences decreasing the acidity in fruit throughout the growing season and increase firmness only during the early stage of production.

  6. Rheological properties of strawberry fruit coating with methylcellulose

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    Z Nadim

    2016-04-01

    Full Text Available Introduction: The maintenance of the quality of fresh products is still a major challenge for the consumers. The most important quality attributes contributing to the marketability of fresh fruit include appearance, color, texture, flavor, nutritional value and microbial safety. Strawberry fruits should be firm but not crunchy. Decreased quality during postharvest handling is most often associated with water loss and decay. The postharvest life of strawberries can be extended by coating technique combined with refrigeration. Application of edible coatings is a conventional method to increase shelf life and maintain fruit quality. Edible coatings can provide an alternative to enlarge fresh fruits’ postharvest life. In this study, the effects of application of methyl cellulose edible coatings and storage time on some mechanical properties, including: the yield stress, yield strain, energy of rupture and modulus of elasticity and also, the viscoelastic behavior of the strawberry fruit was investigated. Materials and Methods: MC (Methocel, Dow Chemical Company, Midland, MI coating was prepared by solubilizing MC powder (3.0 g per 100 mL in a water–ethyl alcohol mixture (2:1 at 75ºC under the high speedmixer (900 rpm for 15 min. Coatings were used directly on the fruit surface. The physical and mechanical characteristics of fruits were analyzed on 2, 5, 8 and 11 days of storage. The puncture test and relaxation test were done using a texture analyzer (Zwick/Roell Model BT1_FR0.5TH.D14, Zwick GmbH Co., Ulm, Germany; using Xforce HP model of loadcell with capacity of 500 N, by 2 mv/v characteristic. General Maxwell model is widely used to analyze experimental results of the stress tests applied for relaxation. The obtained model coefficients were determined and evaluated from relaxation stress curves. Residues were determined using the sequential model. Usually, multicomponent models can properly describe the actual behavior of agricultural

  7. INFLUENCE OF CHEMICAL FERTILIZATION ON STRAWBERRY FRUIT PRODUCTION

    Directory of Open Access Journals (Sweden)

    Alina Florea

    2014-12-01

    Full Text Available The nutritional control of strawberry is very difficult, because a large number of factors influence the absorption and availability of nutrients through mechanisms that are not fully understood. Aim is to establish concrete measures for improving crop technology by applying optimum dose of fertilizers, leading to increased productivity and fruit quality of strawberry culture. The research was conducted during 2009-2011, in a two-way experience. It was located at the Institute of Research - Institute for Fruit Growing Pitesti. Experimental factors were: A - cultivars: a1 - Onebor, a2 – Real, a3 - Elsanta a3, a4 - Magic, a5 - Alba, a6 – Premial; a7-Queen Elisa; B – Fertilizer with 6 graduations: b1 - unfertilized, and five graduations b2 - b6 were applied mixtures of the below fertilizers, thus: b2 - 6.4 kg/ha/week, b3 - 14.3 kg/ha/week, b4 - 21.4 kg/ha/week, b5 - 28.5 kg/ha/week and b6 - 35.8 kg/ha/week. The fertilizers applied were: Polyfeed (N19: P19: K19, potassium nitrate (N12: P0: K43: Mg2 and Magnisal (N11: P0: K0: Mg15. Mixtures were applied at 2 weeks intervals, as follows: 1 April to 15 May (flowering time, 2 applications, containing macroelements N: P: K: Mg - 14:6,3:20,7:5,7 g/100 g mixed fertilizers; May 15 - June 15 (in the picking period, 2 applications of N: P: K: Mg 10,2:6,3:13,5:2,83 g/100 g blend fertilizer and August 1 to September 15 (2 applications with N: P: K: Mg 12,2:6,3:20,7:31,6 g/100 g mixed fertilizers. Following determinations were made: number of fruit per plant, average weight of fruit (g by weighing a sample of 250 fruits and production in tons/hectare. Experimental treatments have led to differences in fruit production. Thus, the analysis of fertilizer doses on the chemical components of the fruits of the seven studied cultivars, we concluded that V5 and V6 treatments had a positive influence on number of fruit per plant and on yielding capacity. Treatments induced also, highest average fruit weight in

  8. Plant probiotic bacteria Bacillus and Paraburkholderia improve growth, yield and content of antioxidants in strawberry fruit.

    Science.gov (United States)

    Rahman, Mosaddiqur; Sabir, Abdullah As; Mukta, Julakha Akter; Khan, Md Mohibul Alam; Mohi-Ud-Din, Mohammed; Miah, Md Giashuddin; Rahman, Mahfuzur; Islam, M Tofazzal

    2018-02-06

    Strawberry is an excellent source of natural antioxidants with high capacity of scavenging free radicals. This study evaluated the effects of two plant probiotic bacteria, Bacillus amylolequefaciens BChi1 and Paraburkholderia fungorum BRRh-4 on growth, fruit yield and antioxidant contents in strawberry fruits. Root dipping of seedlings (plug plants) followed by spray applications of both probiotic bacteria in the field on foliage significantly increased fruit yield (up to 48%) over non-treated control. Enhanced fruit yield likely to be linked with higher root and shoot growth, individual and total fruit weight/plant and production of phytohormone by the probiotic bacteria applied on plants. Interestingly, the fruits from plants inoculated with the isolates BChi1 and BRRh-4 had significantly higher contents of phenolics, carotenoids, flavonoids and anthocyanins over non-treated control. Total antioxidant activities were also significantly higher (p < 0.05) in fruits of strawberry plants treated with both probiotic bacteria. To the best of our knowledge, this is the first report of significant improvement of both yield and quality of strawberry fruits by the application of plant probiotic bacteria BChi1 and BRRh-4 in a field condition. Further study is needed to elucidate underlying mechanism of growth and quality improvement of strawberry fruits by probiotic bacteria.

  9. THE IMPACT OF ENVIRONMENTALLY FRIENDLY POSTHARVEST TREATMENTS ON THE ANTIOXIDANT ACTIVITY OF STRAWBERRY FRUITS DURING STORAGE

    Directory of Open Access Journals (Sweden)

    Ivna Štolfa

    2014-12-01

    Full Text Available Proper postharvest storage is an effective way to maintain the quality and nutritional values of fruits. The aim of this study was to determine how environmentally friendly postharvest treatments with salicylic acid solution, colloidal silver solution and ozone, affect the antioxidant activity of strawberry fruits (Fragaria x ananassa Duch. cv. Albion during 7 days of storage at 4°C. The content of ascorbic acid, total phenols and antioxidant activity of strawberry fruits were determined spec-trophotometrically. After 7 days of storage in strawberry fruits treated with all three treatments separately, the contents of ascorbic acid were higher than in the control fruits, supporting the usefulness of these treatments for preserving fruit quality and nutritional value during storage. The treatment with salicylic acid solution showed the most beneficial effect during storage causing a significant increase in the content of ascorbic acid, phenols and antioxidant activity at the end of the storage period.

  10. Abscisic acid and sucrose regulate tomato and strawberry fruit ripening through the abscisic acid-stress-ripening transcription factor.

    Science.gov (United States)

    Jia, Haifeng; Jiu, Songtao; Zhang, Cheng; Wang, Chen; Tariq, Pervaiz; Liu, Zhongjie; Wang, Baoju; Cui, Liwen; Fang, Jinggui

    2016-10-01

    Although great progress has been made towards understanding the role of abscisic acid (ABA) and sucrose in fruit ripening, the mechanisms underlying the ABA and sucrose signalling pathways remain elusive. In this study, transcription factor ABA-stress-ripening (ASR), which is involved in the transduction of ABA and sucrose signalling pathways, was isolated and analysed in the nonclimacteric fruit, strawberry and the climacteric fruit, tomato. We have identified four ASR isoforms in tomato and one in strawberry. All ASR sequences contained the ABA stress- and ripening-induced proteins and water-deficit stress-induced proteins (ABA/WDS) domain and all ASR transcripts showed increased expression during fruit development. The expression of the ASR gene was influenced not only by sucrose and ABA, but also by jasmonic acid (JA) and indole-3-acetic acid (IAA), and these four factors were correlated with each other during fruit development. ASR bound the hexose transporter (HT) promoter, which contained a sugar box that activated downstream gene expression. Overexpression of the ASR gene promoted fruit softening and ripening, whereas RNA interference delayed fruit ripening, as well as affected fruit physiological changes. Change in ASR gene expression influenced the expression of several ripening-related genes such as CHS, CHI, F3H, DFR, ANS, UFGT, PG, PL, EXP1/2, XET16, Cel1/2 and PME. Taken together, this study may provide new evidence on the important role of ASR in cross-signalling between ABA and sucrose to regulate tomato and strawberry fruit ripening. The findings of this study also provide new insights into the regulatory mechanism underlying fruit development. © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  11. Evaluation of quality parameters of strawberry fruits in modified ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-03-06

    Mar 6, 2009 ... postharvest physiology of strawberry. In: Luby JJ, Dale A (Eds.), The. Strawberry into the 21th Century. Timber Press, Portland, OR, pp. 145-152. Kovach J, Petzoldt R, Harman GE (2000). Use honey bees and bumble bees to disseminate Trichoderma harzianum 1295-22 to straw- berries for botrytis control.

  12. An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

    NARCIS (Netherlands)

    Medina-Puche, L.; Molina-Hidalgo, F.J.; Boersma, M.; Schuurink, R.C.; López-Vidriero, I.; Solano, R.; Franco-Zorrilla, J.M.; Caballero, J.L.; Blanco-Portales, R.; Muñoz-Blanco, J.

    2015-01-01

    Eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. In ripe strawberry (Fragaria x ananassa) fruit receptacles, eugenol is biosynthesized by eugenol synthase (FaEGS2). However, the transcriptional regulation of this process is still unknown. We have identified and

  13. Control of postharvest Botrytis fruit rot of strawberry by volatile organic compounds of Candida intermedia.

    Science.gov (United States)

    Huang, R; Li, G Q; Zhang, J; Yang, L; Che, H J; Jiang, D H; Huang, H C

    2011-07-01

    A study was conducted to identify volatile organic compounds or volatiles produced by Candida intermedia strain C410 using gas chromatography-mass spectrometry, and to determine efficacy of the volatiles of C. intermedia in suppression of conidial germination and mycelial growth of Botrytis cinerea and control of Botrytis fruit rot of strawberry. Results showed that, among 49 volatiles (esters, alcohols, alkenes, alkanes, alkynes, organic acids, ketones, and aldehydes) identified from C. intermedia cultures on yeast extract peptone dextrose agar, two compounds, 1,3,5,7-cyclooctatetraene and 3-methyl-1-butanol, were the most abundant. Synthetic chemicals of 1,3,5,7-cyclooctatetraene; 3-methyl-1-butanol; 2-nonanone; pentanoic acid, 4-methyl-, ethyl ester; 3-methyl-1-butanol, acetate; acetic acid, pentyl ester; and hexanoic acid, ethyl ester were highly inhibitory to conidial germination and mycelial growth of B. cinerea. Inhibition of conidial germination and mycelial growth of B. cinerea by volatiles of C. intermedia was also observed. Meanwhile, results showed that incidence and severity of Botrytis fruit rot of strawberry was significantly (P intermedia cultures or C. intermedia-infested strawberry fruit. These results suggest that the volatiles of C. intermedia C410 are promising biofumigants for control of Botrytis fruit rot of strawberry.

  14. Nutritional and nutraceutical comparison of Jamaican Psidium cattleianum (strawberry guava) and Psidium guajava (common guava) fruits.

    Science.gov (United States)

    McCook-Russell, Kayanne P; Nair, Muraleedharan G; Facey, Petrea C; Bowen-Forbes, Camille S

    2012-09-15

    Psidium cattleianum (strawberry guava) is one of many underutilised edible fruits that grow wild in Jamaica, and could potentially be commercially exploited to yield health and economic benefits. In this study, the total phenolics, proximate contents, and antioxidant, anti-inflammatory, and antimicrobial activities of P. cattleianum and P. guajava (common guava), a well-known species, were compared. Strawberry guavas were found to be superior to common guavas in antioxidant and antimicrobial activities, total phenolics and vitamin C content. They also possessed relatively high fibre content (24.9%). The hexane and ethyl acetate extracts of strawberry guavas showed cyclooxygenase-2 enzyme inhibitory activities of 18.3% and 26.5%, respectively (250 μg/mL), indicating anti-inflammatory activity. The EtOAc and MeOH extracts of P. guajava showed 56.4% (COX-2) and 44.1% (COX-1) inhibitory activity, respectively. Additionally, nine compounds were isolated from strawberry guava fruits, some of which demonstrated anti-inflammatory activity. These results indicate that strawberry guavas are beneficial for health. Copyright © 2012 Elsevier Ltd. All rights reserved.

  15. Transcriptome profiling of postharvest strawberry fruit in response to exogenous auxin and abscisic acid.

    Science.gov (United States)

    Chen, Jingxin; Mao, Linchun; Lu, Wenjing; Ying, Tiejin; Luo, Zisheng

    2016-01-01

    Auxin and abscisic acid regulate strawberry fruit ripening and senescence through cross-talk of their signal transduction pathways that further modulate the structural genes related to physico-chemical properties of fruit. The physiological and transcriptomic changes in harvested strawberry fruits in responses to IAA, ABA and their combination were analyzed. Exogenous IAA delayed the ripening process of strawberries after harvest while ABA promoted the postharvest ripening. However, treatment with a combination of IAA and ABA did not slow down nor accelerate the postharvest ripening in the strawberry fruits. At the molecular level, exogenous IAA up regulated the expressions of genes related to IAA signaling, including AUX/IAA, ARF, TOPLESS and genes encoding E3 ubiquitin protein ligase and annexin, and down regulated genes related to pectin depolymerization, cell wall degradation, sucrose and anthocyanin biosyntheses. In contrast, exogenous ABA induced genes related to fruit softening, and genes involved in signaling pathways including SKP1, HSPs, CK2, and SRG1. Comparison of transcriptomes in responses to individual treatments with IAA or ABA or the combination revealed that there were cooperative and antagonistic actions between IAA and ABA in fruit. However, 17% of the differentially expressed unigenes in response to the combination of IAA and ABA were unique and were not found in those unigenes responding to either IAA or ABA alone. The analyses also found that receptor-like kinases and ubiquitin ligases responded to both IAA and ABA, which seemed to play a pivotal role in both hormones' signaling pathways and thus might be the cross-talk points of both hormones.

  16. Etiology and Population Genetics of Colletotrichum spp. Causing Crown and Fruit Rot of Strawberry.

    Science.gov (United States)

    Ureña-Padilla, A R; Mackenzie, S J; Bowen, B W; Legard, D E

    2002-11-01

    ABSTRACT Isolates of Colletotrichum spp. from diseased strawberry fruit and crowns were evaluated to determine their genetic diversity and the etiology of the diseases. Isolates were identified to species using polymerase chain reaction primers for a ribosomal internal transcribed spacer region and their pathogenicity was evaluated in bioassays. Isolates were scored for variation at 40 putative genetic loci with random amplified polymorphic DNA and microsatellite markers. Only C. acutatum was recovered from diseased fruit. Nearly all isolates from crowns were C. gloeosporioides. In crown bioassays, only isolates of C. gloeosporioides from strawberry caused collapse and death of plants. A dendrogram generated from the genetic analysis identified several primary lineages. One lineage included isolates of C. acutatum from fruit and was characterized by low diversity. Another lineage included isolates of C. gloeosporioides from crowns and was highly polymorphic. The isolates from strawberry formed distinctive clusters separate from citrus isolates. Evaluation of linkage disequilibrium among polymorphic loci in isolates of C. gloeosporioides from crowns revealed a low level of disequilibrium as would be expected in sexually recombining populations. These results suggest that epidemics of crown rot are caused by Glomerella cingulata (anamorph C. gloeosporioides) and that epidemics of fruit rot are caused by C. acutatum.

  17. Comparative study of aromatic compounds in fruit wines from raspberry, strawberry, and mulberry in central Shaanxi area

    Directory of Open Access Journals (Sweden)

    Yiming Feng

    2015-11-01

    Full Text Available Background: Although grape wines have firmly dominated the production and consumption markets of fruit wines, raspberry, strawberry, and mulberry have been utilized to make wines because of their joyful aroma and high contents of polyphenolic phytochemicals and essential fatty acids. However, little is known about aromatic compounds of the wines produced from these three fruits. Methods: The aromatic composition of fruit wines produced from raspberry, strawberry, mulberry, and red grape was analyzed by GC-MS. Odor activity values (OAVs and relative odor contributions (ROCs were used to estimate the sensory contribution of the aromatic compounds to the overall flavor of the wines. Results: In strawberry, raspberry, and mulberry wines, 27, 30, and 31 odorants were detected, respectively. Alcohols formed the most abundant group, followed by esters and acids. The grape wine contained a wider variety (16 types of alcohols, and 4-methyl-2-pentanol and 2,3-butanediol were not present in the three fruit wines. The quantity of esters in raspberry (1.54% and mulberry wines (2.08% were higher than those of strawberry wine (0.78%, and mulberry wine contained more types of esters. There were no significant differences of acids between the three fruit wines and the control wine. In addition, 2-heptanone, 2-octanone, 2-nonanone, and 2-undecanone were unique to raspberry wine, and nonanal was present only in mulberry wine. The indistinguishable aroma of the three fruit wines was attributed to the dominance of fruity and floral odor components derived from ethyl esters of fatty acids and their contributions to the global aroma of the three fruit wines. Conclusion: The present study demonstrated that there were significant differences in the volatile components of fruit wines made from raspberry, strawberry, and mulberry. The aroma compounds were more abundant in the raspberry and mulberry wines than in the strawberry wine, but the quality of strawberry wine was

  18. Effect of biological sprays on the incidence of grey mould, fruit yield and fruit quality in organic strawberry production

    Directory of Open Access Journals (Sweden)

    S. PROKKOLA

    2008-12-01

    Full Text Available Plant diseases, especially grey mould (Botrytis cinerea, may cause severe losses in organic strawberry production. In a two-year period, 2001–2002, the effects of different biological sprays on grey mould, the fruit yield and fruit quality of organically grown strawberry ‘Jonsok’ were studied in field trials at MTT Agrifood Research Finland in Ruukki and Mikkeli. In Experiment 1 the biological sprays were seaweed, garlic and compost extracts, silicon and Trichoderma spp. on both trial sites. In Experiment 2, compost extract, Trichoderma spp. and Gliocladium catenulatum sprays were studied in Ruukki. The treatment time was chosen to control grey mould. The effect of different biological sprays on the incidence of grey mould and total and marketable yield was insignificant compared to the untreated control. In both years and in all trials the incidence of grey mould was low and rot occurred mainly in the latter part of the harvesting period, which may partly explain the small differences between treatments. Anyhow, despite of feasible biological control cultural control methods will be important to manage the fungus in organic strawberry production.;

  19. Antioxidant properties and neuroprotective capacity of strawberry tree fruit (Arbutus unedo).

    Science.gov (United States)

    Fortalezas, Sofia; Tavares, Lucélia; Pimpão, Rui; Tyagi, Meenu; Pontes, Vera; Alves, Paula M; McDougall, Gordon; Stewart, Derek; Ferreira, Ricardo B; Santos, Cláudia N

    2010-02-01

    Berries contain significant amounts of phytochemicals, including polyphenols, which are reported to reduce cancer risk, coronary heart disease and other degenerative diseases. These effects are mainly attributed to the antioxidant capacity of polyphenols found in berries. Strawberry tree (Arbutus unedo) berries are used in folk medicine but seldom eaten as fresh fruits. Their phenolic profile and antioxidant capacity reveal a high potential, but they are not well characterized as a "health promoting food". The aim of this study was to assess the antioxidant properties of the edible strawberry tree fruit in vitro and in a neurodegeneration cell model. Raspberry (Rubus idaeus), a well documented health-promoting fruit, was used as a control for comparison purposes. A. unedo yielded a similar content in polyphenols and a slightly lower value of total antioxidant capacity in comparison to R. idaeus. Although the chemically-measured antioxidant activity was similar between both fruits, R. idaeus increased neuroblastoma survival in a neurodegeneration cell model by 36.6% whereas A. unedo extracts caused no effect on neuroblastoma viability. These results clearly demonstrate that a promising level of chemically-determined antioxidant activity of a plant extract is not necessarily correlated with biological significance, as assessed by the effect of A. unedo fruit in a neurodegeneration cell model.

  20. The Content of Secondary Metabolites and Antioxidant Activity of Wild Strawberry Fruit (Fragaria vesca L.

    Directory of Open Access Journals (Sweden)

    Magdalena Dyduch-Siemińska

    2015-01-01

    Full Text Available Chemical analyses carried out in 2011–2013 aimed at evaluating the contents of flavonoids, free phenolic acids, tannins, anthocyanins, and antioxidant activity (% by means of DPPH radical neutralization ability in fresh and air-dried fruits of three wild strawberry cultivars. Examinations revealed differences in contents of biologically active substances determined in raw versus dried material depending on the cultivar. Mean concentrations of flavonoids and tannins were highest in raw fruits of “Baron von Solemacher” cv., which amounted to 1.244 mg·g−1 and 6.09%, respectively. Fresh fruits of “Regina” cv. were characterized by the highest average content of phenolic acids and anthocyanins: 4.987 mg·g−1 and 0.636 mg·100 g−1. The pattern of mean contents of biologically active substances analyzed in air-dried fruits was similar. Significant differences in abilities to neutralize the DPPH radical to diphenylpicrylhydrazine by extracts made of examined wild strawberry fruits were also indicated.

  1. Antioxidant Properties and Neuroprotective Capacity of Strawberry Tree Fruit (Arbutus unedo

    Directory of Open Access Journals (Sweden)

    Ricardo B. Ferreira

    2010-02-01

    Full Text Available Berries contain significant amounts of phytochemicals, including polyphenols, which are reported to reduce cancer risk, coronary heart disease and other degenerative diseases. These effects are mainly attributed to the antioxidant capacity of polyphenols found in berries. Strawberry tree (Arbutus unedo berries are used in folk medicine but seldom eaten as fresh fruits. Their phenolic profile and antioxidant capacity reveal a high potential, but they are not well characterized as a “health promoting food”. The aim of this study was to assess the antioxidant properties of the edible strawberry tree fruit in vitro and in a neurodegeneration cell model. Raspberry (Rubus idaeus, a well documented health-promoting fruit, was used as a control for comparison purposes. A. unedo yielded a similar content in polyphenols and a slightly lower value of total antioxidant capacity in comparison to R. idaeus. Although the chemically-measured antioxidant activity was similar between both fruits, R. idaeus increased neuroblastoma survival in a neurodegeneration cell model by 36.6% whereas A. unedo extracts caused no effect on neuroblastoma viability. These results clearly demonstrate that a promising level of chemically-determined antioxidant activity of a plant extract is not necessarily correlated with biological significance, as assessed by the effect of A. unedo fruit in a neurodegeneration cell model.

  2. Maturity stages affect the postharvest quality and shelf-life of fruits of strawberry genotypes growing in subtropical regions

    Directory of Open Access Journals (Sweden)

    M. Moshiur Rahman

    2016-01-01

    Full Text Available The postharvest changes of five promising strawberry genotypes viz. Sweet Charlie, Festival, Camarosa, FA 008 and BARI Strawberry-1 at ambient temperature were studied under sub tropical region during the winter season (December–April of 2010–2011 and 2011–2012 in Bangladesh. Irrespective of maturity stages percent fruit decay and weight of fruits were noted minimum in Camarosa and maximum in FA 008 up to day 4 of storage. The shelf life of fruits was maximum in Camarosa and minimum in FA 008 and BARI Strawberry-1 regardless of maturity stage throughout the storage period. The TSS, total sugar and ascorbic acid content of fruits were increased with the increase in maturity stage during the storage period. In 1/3rd and 2/3rd maturity stages, the TSS and total sugar content were found the highest in Festival but at full maturity stage those were recorded higher in Camarosa. The titratable acidity was noticed the highest in 1/3rd matured fruits and gradually decreased with the increase in maturity stage as well as storage duration in all the genotypes. Ascorbic acid content of strawberry gradually decreases during the storage period. Fully matured fresh fruits of Festival contained maximum ascorbic acid content while BARI Strawberry-1 contained minimum ascorbic acid that was reduced after 3 days of storage.

  3. Influence of Irradiated Chicken Manure on Productivity and Fruit Quality of Strawberries

    International Nuclear Information System (INIS)

    Fath El-Bab, T.Sh.

    2014-01-01

    A field experiment was carried out on Strawberry fruits (Fragaria×ananassa) cv. camarosa at Atomic Energy Authority, Experimental farm, Inshas, Egypt during the two successive seasons 2011 and 2012. Chicken manure at rates of 15 and 30 m 3 fed -1 were irradiated with 10 KGy gamma were applied in combination with 206 N + 31 P 2 O 5 + 240 K 2 O unit fed -1 . Untreated control but fertilized with 206 N + 31 P 2 O 5 + 240 K 2 O unit fed-1was also included. Generally chicken manure rates significantly increased vegetative growth, and total yield quality of strawberry fruits. The superiority data with 30 m 3 fed -1 irradiated chicken manure was observed on strawberry of plant height, number of leaves plant, and number of crowns plant, root length and dry weight of shoots. Also total soluble solids and acidity, vitamin C, total sugars and anthocyanin content were significantly increased comparable to control. Nitrogen, phosphorus and potassium contents non significantly affected most of treatments except the combined treatment of chicken manure at rate 30 m 3 fed -1 and 206 N + 31 P 2 O 5 + 240 K 2 O unit fed -1 that induced the best results. This was true at the 2nd season. Moreover these results were nearly closed those of irradiated dry chicken manure at rate of 30 m 3 fed -1 plus 206 N + 31 P 2 O 5 + 240 K 2 O unit fed -1 , for both seasons

  4. Cancer-Related Constituents of Strawberry Jam as Compared with Fresh Fruit

    Directory of Open Access Journals (Sweden)

    Gema Flores

    2016-01-01

    Full Text Available The health awareness recently shown by consumers has led to a demand for health beneficial products. In particular, researchers are currently focusing their studies on the search for foods for cancer prevention activity. In the present work, we study comparatively the effect of two different processing methods on the contents of phenolic compounds (i.e., ellagic acid, myricetin, quercetin and kaempferol with antioxidant and antitumor properties in strawberry jams. In turn, the results obtained were compared with those of unprocessed fruit. Additionally carcinogenic heat-induced compounds formed by the two jam making methods were evaluated. Decreases of total ellagic acid from 138.4 µg/g to 86.5 µg/g were measured in jam as compared with the intact fruit. Even higher losses of up to 90% of total flavonols were found in strawberry after the jam-making process. A comparison between the two processing methods proved shorter heating periods (around 60 min even at temperatures as high as 100 °C enabled losses of antioxidant phenolics to be minimized. Carcinogenic heat-induced volatile compounds, mainly Maillard reaction products, were formed as a result of thermal treatment during jam processing. However, shorter heating periods also helped reduce the formation of these harmful compounds. These results are deeply discussed. From a practical standpoint, the processing conditions here proposed can be used by industry to obtain strawberry jam with higher content of antioxidant flavonoids and, at the same time, reduced amounts of carcinogenic compounds.

  5. Aroma biosynthesis in strawberry: s-adenosylmethionine:furaneol o-methyltransferase activity in ripening fruits.

    Science.gov (United States)

    Lavid, Noa; Schwab, Wilfried; Kafkas, Ebru; Koch-Dean, Margery; Bar, Einat; Larkov, Olga; Ravid, Uzi; Lewinsohn, Efraim

    2002-07-03

    Among the most important volatile compounds in the aroma of strawberries are 2,5-dimethyl-4-hydroxy-3(2H)-furanone (Furaneol) and its methoxy derivative (methoxyfuraneol, mesifuran). Three strawberry varieties, Malach, Tamar, and Yael, were assessed for total volatiles, Furaneol, and methoxyfuraneol. The content of these compounds sharply increased during fruit ripening, with maximum values at the ripe stage. An enzymatic activity that transfers a methyl group from S-adenosylmethionine (SAM) to Furaneol sharply increases during ripening of strawberry fruits. The in vitro generated methoxyfuraneol was identified by radio-TLC and GC-MS. The partially purified enzyme had a native molecular mass of approximately 80 kDa, with optimum activity at pH 8.5 and 37 degrees C. A high apparent K(m) of 5 mM was calculated for Furaneol, whereas this enzyme preparation apparently accepted as substrates other o-dihydroxyphenol derivatives (such as catechol, caffeic acid, and protocatechuic aldehyde) with much higher affinities (K(m) approximately 105, 130, and 20 microM, respectively). A K(m) for SAM was found to be approximately 5 microM, regardless of the acceptor used. Substrates that contained a phenolic group with only one OH group, such as p-coumaric and trans-ferulic acid, as well as trans-anol and coniferyl alcohol, were apparently not accepted by this activity. It is suggested that Furaneol methylation is mediated by an O-methyltransferase activity and that this activity increases during fruit ripening.

  6. The physico-chemical properties of strawberry tree (Arbutus unedo L. fruits

    Directory of Open Access Journals (Sweden)

    J. Hribar

    2013-01-01

    Full Text Available The physico-chemical properties of ripe fruits of strawberry tree (Arbutus unedo L. were determined. The water content, ash, crude fat, proteins, total phenols, sugar, and the content of vitamin C were determined in ripe strawberry tree fruits. Fruits contain 46.7 % of water, 23.5 % of soluble solids, 0.48 % of ash, 118.61 mg/100 g of potassium, 20.63 mg/100 g of sodium, 36.05 mg/100 g of calcium, 9.66 mg/100 g of magnesium, 1.29 mg/100 g of iron, 19.99 mg/100 g of phosphorus, 0.45 mg/100 g of zinc, < 0.99 mg/100 g of manganese, < 0.99 mg/100 g of chromium, < 0.10 mg/100 g of nickel, < 1.32 mg/100 g of lead and < 0.10 mg/100 g of cadmium. Among nutritionally important components found in fruits were: total fat (0.43 %, proteins (0.82 %, fibres (18.5 g/100 g of which 14.3 g/100g was insoluble and 4.19 g/100 g was soluble fibre, titratable acids (5.1 mg/100 g, glucose (6.2 g/100 g and fructose (17.2 g/100 g. Ripe fruits contained 271.5 mg/100 g vitamin C, of which 255.3 mg/ 100 g was L-ascorbic acid and 16.2 mg/100 g was dehydroascorbic acid.

  7. Effect of organic amendments on vegetative growth, fruit and yield quality of strawberry

    International Nuclear Information System (INIS)

    Khalid, S.; Qureshi, K.M.; Hafiz, I.A.; Khan, K.S.; Qureshi, U.S.

    2013-01-01

    Organic agricultural techniques are utilized globally to protect our environment and prevent health issues resulting from pesticides and hazardous chemicals. In this regard, studies were conducted using six different organic amendments on strawberry (Fragaria ananassa Duch.) cv. Chandler which included T 1 = planting media (soil + silt + farm yard manure); T 2 = planting media + 400 mgl/sup -1/ humic acid; T 3 = planting media + 200 g kg/sup -1/ leaf manure; T 4 = planting media + 200 g kg/sup -1/ vermicompost; T 5 = planting media + 200 g kg/sup -1/ plant fertilizer and T 6 = planting media + 200 g kg/sup -1/ bio-compost during 2011-12 at PMAS-Arid Agriculture University, Rawalpindi. Treatment T 1 (soil + silt + FYM) induced positive influence on plant height (15.21 cm), canopy spread (20.37 cm), crown diameter (1.47 cm), fresh weight of plant (10.71 g), number of runners per plant (2), total number of flowers (58), total number of fruits (42), fruit size (3.04 cm), fruit weight per berry (8.82 g) while T 4 (soil + silt + 200 g/sup -1/ kg vermicompost) improved fresh leaf weight (0.92 g), number of leaves (6.67), leaf area (43.07 cm/sup 2/) and days required for first bloom (96.67). Leaf manure based treatment (T 3 ) enhanced root length (20.11 cm), T 4 improved quality parameters like total soluble solid (TSS) (8.88) and ascorbic acid contents (64 mg) while T 1 improved total sugar contents in fruits (6.82%). Hence farm yard manure(FYM) and vermicompost based organic amendments enhanced vegetative growth and improved quality of strawberry fruits. (author)

  8. Aroma Quality of Fruits of Wild and Cultivated Strawberry (FRAGARIA SPP. in Relation to the Flavour-Related Gene Expression

    Directory of Open Access Journals (Sweden)

    Bianchi Giulia

    2014-09-01

    Full Text Available Expression profiles of flavour-related genes and the aroma quality of fruit headspace were investigated in the four strawberry genotypes ‘Reine des Vallées’ (Fragaria vesca, ‘Profumata di Tortona’ (F mos-chata, ‘Onda’ and VR 177 selection (F” x ananassa. Differences in the expression level of genes coding of strawberry alcohol acyltransferase (SAAT, F. x ananassa nerolidol synthase 1 (FaNESl and F vesca monoterpene and sesquiterpene synthases (FvPINS and PINS1, respectively were detected among these genotypes. In fruits of F. x ananassa the terpenoid profile was dominated by nerolidol, whereas wild spe–cies produced mainly monoterpenes. It was correlated with the higher induction of FaNES1 in cultivated and PINS gene in the wild Fragaria species. The flavour biogenesis in ripening fruits was determined by the expression of SAAT gene, especially visible for ‘Profumata di Tortona’ and ‘Onda’ strawberries. The fruit solid-phase microextraction (SPME headspace was analysed using the Gas Chromatography-Olfac–tometry (GC-O, that allows for the chromatographic separation of volatiles together with their olfactomet-ric evaluation. ‘Reine des Vallées’ fruits have a peculiar profile characterized by high concentrations of limonene, linalool and mesifurane that resulted in “spiced”, “citrus, floral” and “sweet, baked” descriptors. The character impact compound in ‘Profumata di Tortona’ fruits was ethyl butanoate, responsible for “sweet” and “fruity, strawberry” descriptors. However, it was detected in lower amount in comparison to the data obtained for F. x ananassa strawberries. The sesquiterpene nerolidol was identified in both culti–vated strawberry genotypes.

  9. 77 FR 22557 - Notice of Availability of a Pest Risk Analysis for the Importation of Strawberry Fruit From Egypt...

    Science.gov (United States)

    2012-04-16

    ... Inspection Service [Docket No. APHIS-2012-0009] Notice of Availability of a Pest Risk Analysis for the... the public that we have prepared a pest risk analysis that evaluates the risks associated with the... importation of fresh strawberry fruit from Egypt. We are making the pest risk analysis available to the public...

  10. 78 FR 13304 - Notice of Decision To Issue Permits for the Importation of Strawberry Fruit From Egypt Into the...

    Science.gov (United States)

    2013-02-27

    ... continental United States of fresh strawberry fruit from Egypt. Based on the findings of a pest risk analysis... importation of commodities that, based on the findings of a pest risk analysis (PRA), can be safely imported... risks of introducing or disseminating plant pests or noxious weeds via the importation of fresh...

  11. Selenium Biofortification in Fragaria × ananassa: Implications on Strawberry Fruits Quality, Content of Bioactive Health Beneficial Compounds and Metabolomic Profile

    Directory of Open Access Journals (Sweden)

    Tanja Mimmo

    2017-11-01

    Full Text Available Selenium (Se is an essential nutrient for humans, due to its antioxidant properties, whereas, to date, its essentiality to plants still remains to be demonstrated. Nevertheless, if added to the cultivation substrate, plants growth resulted enhanced. However, the concentration of Se in agricultural soils is very variable, ranging from 0.01 mg kg-1 up to 10 mg kg-1 in seleniferous areas. Therefore several studies have been performed aimed at bio-fortifying crops with Se and the approaches exploited were mainly based on the application of Se fertilizers. The aim of the present research was to assess the biofortification potential of Se in hydroponically grown strawberry fruits and its effects on qualitative parameters and nutraceutical compounds. The supplementation with Se did not negatively affect the growth and the yield of strawberries, and induced an accumulation of Se in fruits. Furthermore, the metabolomic analyses highlighted an increase in flavonoid and polyphenol compounds, which contributes to the organoleptic features and antioxidant capacity of fruits; in addition, an increase in the fruits sweetness also was detected in biofortified strawberries. In conclusion, based on our observations, strawberry plants seem a good target for Se biofortification, thus allowing the increase in the human intake of this essential micronutrient.

  12. Developing a strawberry yogurt fortified with marine fish oil

    Science.gov (United States)

    Fortified dairy products appeal to a wide variety of consumers and have the potential to increase sales in the yogurt industry and contribute to boost the intake of omega-3 fatty acids. The objectives of this study were to develop a strawberry yogurt containing microencapsulated salmon oil (2% w/v) ...

  13. Effect of irradiation with black light fluorescent lamp on coloration and hardness of strawberry [Fragaria ananassa] fruits

    International Nuclear Information System (INIS)

    Higashio, H.; Hirono, H.; Sato, F.; Tokuda, S.; Uragami, A.

    2009-01-01

    The effect of irradiation by black light fluorescent lamp on the coloration and hardness of detached strawberry fruits was studied. Only the coloring of fruits (var. Toyonoka) that had started to pigment was accelerated by lamp irradiation. Maintaining the irradiation distance and ambient temperature after treatment was very important to obtain the maximum effect, and the action of lamps was restricted to the irradiated parts. The accelerating effect of a lamp on coloration was observed in all 3 of other varieties examined, and in some varieties, the level of anthocyanin concentration in fruits was greater than that in ripening stage. However, the lamp did not affect the hardness of fruits

  14. Fruit development and ripening.

    Science.gov (United States)

    Seymour, Graham B; Østergaard, Lars; Chapman, Natalie H; Knapp, Sandra; Martin, Cathie

    2013-01-01

    Fruiting structures in the angiosperms range from completely dry to highly fleshy organs and provide many of our major crop products, including grains. In the model plant Arabidopsis, which has dry fruits, a high-level regulatory network of transcription factors controlling fruit development has been revealed. Studies on rare nonripening mutations in tomato, a model for fleshy fruits, have provided new insights into the networks responsible for the control of ripening. It is apparent that there are strong similarities between dry and fleshy fruits in the molecular circuits governing development and maturation. Translation of information from tomato to other fleshy-fruited species indicates that regulatory networks are conserved across a wide spectrum of angiosperm fruit morphologies. Fruits are an essential part of the human diet, and recent developments in the sequencing of angiosperm genomes have provided the foundation for a step change in crop improvement through the understanding and harnessing of genome-wide genetic and epigenetic variation.

  15. Sweet, red and Danish: New strawberry varieties on their way

    DEFF Research Database (Denmark)

    Stacey, Julia; Fjord, Thomas Ahle

    2000-01-01

    A strawberry isn't just a strawberry. Some are sweeter than others, more fragrant and juicier. It is therefore a difficult task to develop a strawberry that tastes like in childhood memories of endless sunny days, and which also is suitablefor the distribution system of today. But new varieties...... are on their way. And this time consumers have participated in the development process. The new strawberry varieties are the result of extensive product development in the research project 'Better Danish strawberries' in which the Danish Institute of Agricultural Science, Danish Fruit Growers' Association......, the Danish Veterinary and Food Administration and MAPP participated. Danish strawberry growers initiated the project and the purpose is to assure the quality of strawberries and to improve the distribution and sale. In order to get consumers to evaluate both new and existing varieties the MAPP Centre carried...

  16. Potential link between fruit yield, quality parameters and phytohormonal changes in preharvest UV-C treated strawberry.

    Science.gov (United States)

    Xu, Yanqun; Charles, Marie Thérèse; Luo, Zisheng; Roussel, Dominique; Rolland, Daniel

    2017-07-01

    Preharvest ultraviolet-C (UV-C) treatment of strawberry is a very new approach, and little information is available on the effect of this treatment on plant growth regulators. In this study, the effect of preharvest UV-C irradiations at three different doses on strawberry yield, fruit quality parameters and endogenous phytohormones was investigated simultaneously. The overall marketable yield of strawberry was not affected by the preharvest UV-C treatments, although more aborted and misshapen fruits were found in UV-C treated groups than in the untreated control. The fruits in the high dose group were firmer and had approximately 20% higher sucrose content and 15% higher ascorbic acid content than the control, while fruits from the middle and low dose groups showed no significant changes in these parameters. The lower abscisic acid (ABA) content found in the fruits in the high UV-C group may be associated with those quality changes. The citric acid content decreased only in the low dose group (reduction of 5.8%), with a concomitant 37% reduction in jasmonic acid (JA) content, compared to the control. The antioxidant status of fruits that received preharvest UV-C treatment was considered enhanced based on their oxygen radical absorbance capacity (ORAC) and malondialdehyde (MDA) content. In terms of aroma, three volatile alcohols differed significantly among the various treatments with obvious activation of alcohol acyltransferase (AAT) activity. The observed synchronous influence on physiological indexes and related phytohormones suggests that preharvest UV-C might affect fruit quality via the action of plant hormones. Crown Copyright © 2017. Published by Elsevier Masson SAS. All rights reserved.

  17. Extracts of strawberry fruits induce intrinsic pathway of apoptosis in breast cancer cells and inhibits tumor progression in mice.

    Directory of Open Access Journals (Sweden)

    Ranganatha R Somasagara

    Full Text Available The consumption of berry fruits, including strawberries, has been suggested to have beneficial effects against oxidative stress mediated diseases. Berries contain multiple phenolic compounds and secondary metabolites that contribute to their biological properties.Current study investigates the anticancer activity of the methanolic extract of strawberry (MESB fruits in leukaemia (CEM and breast cancer (T47D cell lines ex vivo, and its cancer therapeutic and chemopreventive potential in mice models. Results of MTT, trypan blue and LDH assays suggested that MESB can induce cytotoxicity in cancer cells, irrespective of origin, in a concentration- and time-dependent manner. Treatment of mice bearing breast adenocarcinoma with MESB blocked the proliferation of tumor cells in a time-dependent manner and resulted in extended life span. Histological and immunohistochemical studies suggest that MESB treatment affected tumor cell proliferation by activating apoptosis and did not result in any side effects. Finally, we show that MESB can induce intrinsic pathway of apoptosis by activating p73 in breast cancer cells, when tumor suppressor gene p53 is mutated.The present study reveals that strawberry fruits possess both cancer preventive and therapeutic values and we discuss the mechanism by which it is achieved.

  18. Fruit quality in strawberry (Fragaria sp. grown on colored plastic mulch

    Directory of Open Access Journals (Sweden)

    Casierra-Posada Fánor

    2011-12-01

    Full Text Available

  19. Effect of Gamma Radiations on the Quality and Shelf Life of Strawberry Fruit of the Uttrakhand Region

    Science.gov (United States)

    Sharma, Prianka; Rastogi, Meetu

    2016-08-01

    Present study was conducted to investigate the effect of gamma radiations on the quality and shelf life of strawberries. The aim of this study was to evaluate gamma radiation doses in range of 0.3- 1.5 kGy. The irradiated strawberries were stored in ambient (temperature 25 +- 2oC, RH 70 %) and refrigerated (3 +-1oC, RH 80%) conditions. In samples treated with dose 1.2-1.5 kGy no decay was recorded up to 9 days of ambient conditions. Under refrigerated conditions, strawberry samples of unirradiated and irradiated in the range of 0.3-0.9 kGy started decaying after 14 days of storage. No decay was observed in the samples treated with 1.2-1.5 kGy up to 28 days of refrigerated storage. Dose of 1.2 kGy was significantly effective in reducing the weight loss and in maintaining the higher overall acceptability under both the storage conditions compared to the other treatments. This dose also proved effective in retention of significantly higher levels of total sugars compared to the other treatments. Thus, it was established that irradiating strawberries with dose of 1.2 kGy can prove beneficial in facilitating the marketing of the fruit to distant places other than the local markets, thereby benefiting the growers.

  20. The role of petals in development of grey mould in strawberries = Importância das pétalas no desenvolvimento do mofo-cinzento do morangueiro

    NARCIS (Netherlands)

    Boff, P.; Kraker, de J.; Gerlagh, de M.; Köhl, J.

    2003-01-01

    Studies were conducted in annual crops of strawberry (Fragaria x ananassa) (cv. Elsanta to assess the relative importance of petals as an inoculum source of grey mould, caused by Botrytis cinerea and to identify during which period of flower and fruit development the presence of petals has a

  1. Response surface optimisation of extraction of antioxidants from strawberry fruit, and lipid peroxidation inhibitory potential of the fruit extract in cooked chicken patties.

    Science.gov (United States)

    Saha, Jayati; Debnath, Moumita; Saha, Arnab; Ghosh, Tanaya; Sarkar, Prabir K

    2011-08-15

    Strawberries contain high levels of antioxidants and have beneficial effects against oxidative stress-mediated diseases, such as cancer. They contain multiple phenolic compounds, which contribute to their biological properties. Hence, a study was carried out to optimise the extraction of antioxidants and evaluate the antioxidant potential of strawberry fruit extract (SE) in cooked chicken patties during refrigerated storage. The activity of SE was compared with that of butylhydroxytoluene (BHT). The effect of solvent type (MeOH and EtOH), concentration (0-70%) of EtOH in the system, temperature (30-60 °C), and time (30-150 min) on DPPH•-scavenging activity of SE was investigated. Response surface methodology was used to estimate the optimum extraction conditions for each parameter. The maximum predicted DPPH• scavenging under the optimised conditions (100% MeOH, 30 °C, 150 min) was 43% at 1 mg SE mL⁻¹. Freshly prepared chicken patties were treated with 5% and 10% SE and 2% BHT, and stored aerobically at 4 °C for 6 days. SE had no influence (P extraction of compounds from strawberry that had the scavenging activity. The study shows that the extraction of natural antioxidants from strawberry can be improved by optimising several key extraction parameters. SE also acted as an effective antioxidant and suppressed lipid peroxidation in cooked chicken patties. Copyright © 2011 Society of Chemical Industry.

  2. The role of FaBG3 in fruit ripening and B. cinerea fungal infection of strawberry.

    Science.gov (United States)

    Li, Qian; Ji, Kai; Sun, Yufei; Luo, Hao; Wang, Hongqing; Leng, Ping

    2013-10-01

    In plants, β-glucosidases (BG) have been implicated in developmental and pathogen defense, and are thought to take part in abscisic acid (ABA) synthesis via hydrolysis of ABA glucose ester to release active ABA; however, there is no genetic evidence for the role of BG genes in ripening and biotic/abiotic stress in fruits. To clarify the role of BG genes in fruit, eight Fa/FvBG genes encoding β-glucosidase were isolated using information from the GenBank strawberry nucleotide database. Of the Fa/FvBG genes examined, expression of FaBG3 was the highest, showing peaks at the mature stage, coincident with the changes observed in ABA content. To verify the role of this gene, we suppressed the expression of FaBG3 via inoculation with Agrobacterium tumefaciens containing tobacco rattle virus carrying a FaBG3 fragment (RNAi). The expression of FaBG3 in FaBG3-RNAi-treated fruit was markedly reduced, and the ABA content was lower than that of the control. FaBG3-RNAi-treated fruit did not exhibit full ripening, and were firmer, had lower sugar content, and were pale compared with the control due to down-regulation of ripening-related genes. FaBG3-RNAi-treated fruit with reduced ABA levels were much more resistant to Botrytis cinerea fungus but were more sensitive to dehydration stress than control fruit. These results indicate that FaBG3 may play key roles in fruit ripening, dehydration stress and B. cinerea fungal infection in strawberries via modulation of ABA homeostasis and transcriptional regulation of ripening-related genes. © 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd.

  3. Comparative Transcriptomics Reveals Differential Gene Expression Related to Colletotrichum gloeosporioides Resistance in the Octoploid Strawberry

    Directory of Open Access Journals (Sweden)

    Feng Wang

    2017-05-01

    Full Text Available The strawberry is an important fruit worldwide; however, the development of the strawberry industry is limited by fungal disease. Anthracnose is caused by the pathogen Colletotrichum gloeosporioides and leads to large-scale losses in strawberry quality and production. However, the transcriptional response of strawberry to infection with C. gloeosporioides is poorly understood. In the present study, the strawberry leaf transcriptome of the ‘Yanli’ and ‘Benihoppe’ cultivars were deep sequenced via an RNA-seq analysis to study C. gloeosporioides resistance in strawberry. Among the sequences, differentially expressed genes were annotated with Gene Ontology terms and subjected to pathway enrichment analysis. Significant categories included defense, plant–pathogen interactions and flavonoid biosynthesis were identified. The comprehensive transcriptome data set provides molecular insight into C. gloeosporioides resistance genes in resistant and susceptible strawberry cultivars. Our findings can enhance breeding efforts in strawberry.

  4. Cytomorphological and autoradiografical studies on the receptacle of the strawberry (Fragaria grandiflora Eherh. in the initial stages of development

    Directory of Open Access Journals (Sweden)

    Jan Szymański

    2013-12-01

    Full Text Available The volume of the cortex and pith cells of strawberries from the cultivar Talizman gradually increased until ripening, especially in the cortex. The volume of the nucleus also increased. The nuclei in the pith were smaller then in the cortex. The volume of the nucleolus increased significantly between days 1 and 5 after pollination, after which it remained unchanged. At the end of the growth period, the volume of the nucleolus in cortical and pith cells in the lower and central part of the fruit increased. Endomitotic polyploidization which was responsible for the growth of the fruit, occurred during the development of the receptacle. The intensity of 3H actinomycin binding reached a maximum in the oldest stages. This is proof for the accumulation of active DNA during mitosis. Saturation with this antibiotic occurred in the early stages of fruit development. No dependence between the volume of cells and the number of nuclei binding 3H actinomycin was observed.

  5. Influence of UV-C irradiation on expansin and pectin-methylesterase gene expression in strawberry fruit

    International Nuclear Information System (INIS)

    Pombo, M.; Dotto, M.; Martinez, G.; Civello, P.

    2005-01-01

    Full text: The exposure to UV-C delays fruit softening, one of the main factors determining fruit post harvest life. This delay in softening may be caused by changes in the activities of enzymes and proteins involved in cell wall disassembly. Expansins are cell wall proteins involved in fruit softening, while pectin-melhylesterases (PME) are cell wall enzymes related to pectin demethylation, and their activity is affected by heating, another physical treatment based on abiotic stress. We analyzed FaPME 1 gene expression in irradiated strawberry fruits and also measured PME activity after treatment. An increase in PME activity immediately after the treatment was found. The expression of FaEXP2, FaEXP4 and FaEXP5 genes was analyzed in the same fruit samples, as well as expansin protein accumulation. For these three genes we found a decrease in expression 4 h after treatment and an increase after 24 h. The decrease in gene expression after treatment correlated with a diminution of expansins. (author)

  6. Effect of some Antioxidants, Caraway Oil (Carium carvum L.) and Gamma Radiation on Gray Mould of Strawberry Fruits during Storage

    International Nuclear Information System (INIS)

    Abd-El-Aziz, Sh.A.; Salem, E.A.

    2011-01-01

    Some antioxidants, caraway oil and gamma radiation were tested against grey mould of strawberry fruits caused by Botrytis cinerea. Salicylic acid was the best antioxidant inhibiting mycelial growth of B. cirerea at 6000 ppm, while the least effective was oxalic acid. Also caraway oil completely inhibited the growth of the fungus at 700 μl/L. Gamma radiation at dose 2.5 kGy caused 87.77% inhibition growth. Light microscope observation revealed that caraway oil at 700 μl/L caused changes in morphological characters of B. cinerea. Antioxidants salicylic acid was recorded the least percentage of infection for fruits naturally or artificially infected with B. cirerea. The least effective was oxalic acid. Caraway oil at 700 μl/L inhibited completely the percentage of infection of fruits naturally or artificially infected with B. cirerea after 4 days of storage, while after 10 days of storage, the percentage of infection for artificially infected fruits was 13.33% and for gamma irradiated fruits were 46.66% at dose 2.5 kGy. Salicylic acid at 8 g/L showed more peroxidase (POD) activity than other treatments

  7. A comparison of bioactive compounds of strawberry fruit from Europe affected by genotype and latitude

    DEFF Research Database (Denmark)

    Josuttis, M.; Carlen, C.; Nestby, R.

    2012-01-01

    The effect of four different growing locations from Stjørdal, Norway (63°36'N) to Conthey, Switzerland (46°12'N) on the composition of bioactives in strawberry (Fragaria × ananassa Duch.) of three genotypes (cvs Clery, Elsanta and Korona) was evaluated. Principal component analysis (PCA) was used...

  8. Comparison of the Effect of 1-MCP and Low-Pressure Air on Shelflife of Strawberry Fruit (Fragaria ananassa cv. Camarossa

    Directory of Open Access Journals (Sweden)

    B. Modares

    2013-10-01

    Full Text Available Strawberry (Fragaria×ananassa has tasteful, fragrant and nutritious fruits. But, because of delicate texture and high metabolic activities of the fruits at maturity, are very sensitive and easily decayed, and thus have short shelflife. Ethylene is one of the main causes of postharvest fruit losses, which could reduce the shelflife of strawberry. Therefore, an experiment was carried out to examine the effect of 1 µL/L 1-MCP treatment and low-pressure air pretreatment at 0.2 atm in 2 hours on apparent and physicochemical characteristics of strawberry fruit, cultivar Camarossa, during storage in cold store. The study was performed as split plots in time, based on complete randomized design with 3 replications. The results showed that the highest marketability (90%, fruit firmness (7.95 N, titrable organic acids (1.15 g/100 cc, vitamin C (78.13 mg/100 cc and total soluble solids (10.48 % and the lowest amount of apparent decay (13.3 %, acidity (3.63 and taste index (9.04 were obtained in 1-MCP and low-pressure air treatments, which shows the effect of treatments on reducing the rate of biochemical variations in fruit texture. Correlation coefficients between all treatments were significant (P≤0.01. Meanwhile, the positive effect of 1-MCP on appearance and studied physicochemical characteristics was more than low-pressure air pretreatment. Even, the combination of these two treatments had no significant difference with 1-MCp treatment alone. In general, this observation depicts the more positive and significant effect of 1-MCP treatment in delaying the maturity and improving the qualitative traits of strawberry fruits after being stored in cold storage.

  9. Assessment of the Role of Local Strawberry Rhizosphere—Associated Streptomycetes on the Bacterially—Induced Growth and Botrytis cinerea Infection Resistance of the Fruit

    Directory of Open Access Journals (Sweden)

    D. İpek Kurtböke

    2010-12-01

    Full Text Available The future need for sustainable agriculture will be met in part by wider use of biological control of plant pathogens over conventional fungicides hazardous to the environment and to public health. Control strategies involving both (i direct use of microorganisms antagonistic to the phytopathogen, and (ii use of bioactive compounds (secondary metabolites/antibiotic compounds from microorganisms on the phytopathogen were both adapted in order to investigate the ability of streptomycetes isolated from the rhizosphere of strawberry plants to promote the growth of the fruit and suppress Botrytis cinerea causing strawberry rot on the Sunshine Coast, Queensland, Australia. In vitro studies showed that 25/39 streptomycetes isolated from strawberry field soils inhibited B. cinerea growth by antifungal activity, ranging from antibiosis to volatile compound production. However, when non-volatile antifungal compounds were extracted and applied aerially to the actively growing strawberry fruits infected with B. cinerea, a significant disease reduction was not recorded. On the other hand, plant and fruit growth was promoted by the presence of actively growing streptomycetes in container media. Findings might indicate that live streptomycete inoculum can be used as growth promoting agent in container media for this economically important crop.

  10. Preharvest Ultraviolet C Irradiation Increased the Level of Polyphenol Accumulation and Flavonoid Pathway Gene Expression in Strawberry Fruit.

    Science.gov (United States)

    Xu, Yanqun; Charles, Marie Thérèse; Luo, Zisheng; Mimee, Benjamin; Veronneau, Pierre-Yves; Rolland, Daniel; Roussel, Dominique

    2017-11-22

    Preharvest ultraviolet C (UV-C) irradiation is an innovative approach for increasing the bioactive phytochemical content of strawberries to increase the disease resistance and nutritional value. This study investigated the changes in individual flavonoids in strawberry developed with three different cumulative doses of preharvest UV-C treatment (low, 9.6 kJ m -2 ; middle, 15 kJ m -2 ; and high , 29.4 kJ m -2 ). Significant accumulation (p radiation. The expression of the flavonoid pathway structural genes, i.e., FaCHS1, FaCHI, FaFHT, FaDFR, FaFLS, and FaFGT, was upregulated in the low- and middle-dose groups, while the early stage genes were not affected by the high dose. FaMYB1 was also relatively enhanced in the low- and middle-dose groups, while FaASR was upregulated in only the low-dose group. Hormetic preharvest UV-C dose ranges for enhancing the polyphenol content of strawberries were established for the first time.

  11. Towards consumer-friendly cisgenic strawberries which are less susceptible to Botrytis cinerea

    NARCIS (Netherlands)

    Schaart, J.G.

    2004-01-01

    This thesis describes the development of genetically modified (GM) strawberries which are less susceptible to fruit rot caused by the fungus Botrytis cinerea. To achieve Botrytis resistance, a polygalacuronase inhibiting protein (PGIP) gene has been isolation from strawberry and was characterised.

  12. Melatonin treatment attenuates postharvest decay and maintains nutritional quality of strawberry fruits (Fragaria×anannasa cv. Selva) by enhancing GABA shunt activity.

    Science.gov (United States)

    Aghdam, Morteza Soleimani; Fard, Javad Rezapour

    2017-04-15

    Fresh strawberry fruits as perishable commodities have a short postharvest life and are prone to postharvest fungal decay. In this study, the impact of 0, 1, 10, 100 and 1000μmol/L melatonin on attenuating fungal decay and maintaining nutritional quality of strawberry fruits was investigated during storage at 4°C for 12days. Melatonin treatment at 100μmol/L triggered H 2 O 2 accumulation, which result from higher superoxide dismutase (SOD) activity, associated with lower catalase (CAT) and ascorbate peroxidase (APX) activities, leading to fruits with lower decay. Higher H 2 O 2 accumulation was concurrent with higher phenylalanine ammonia lyase (PAL) enzyme activity leading to higher total phenols and anthocyanins accumulation along with higher DPPH scavenging capacity. Also, strawberry fruits treated with melatonin exhibited higher γ-aminobutyric acid transaminase (GABA-T) enzyme activity which ensured sufficient ATP supplying leading to higher unsaturated/saturated fatty acids (unSFA/SFA) ratio. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. AM fungi and PGP pseudomonads increase flowering, fruit production, and vitamin content in strawberry grown at low nitrogen and phosphorus levels.

    Science.gov (United States)

    Bona, Elisa; Lingua, Guido; Manassero, Paola; Cantamessa, Simone; Marsano, Francesco; Todeschini, Valeria; Copetta, Andrea; D'Agostino, Giovanni; Massa, Nadia; Avidano, Lorena; Gamalero, Elisa; Berta, Graziella

    2015-04-01

    There is increasing interest in the quality of crops because of the implications concerning health, economic revenue, and food quality. Here we tested if inoculation with a mixture of arbuscular mycorrhizal fungi (AMF) and/or two strains of plant growth-promoting bacteria (PGPB), in conditions of reduced chemical inputs, affects the quality and yield of strawberry fruits. Fruit quality was measured by concentrations of soluble sugars, various organic acids, and two vitamins (ascorbic and folic acid). Co-inoculation with the AMF and each of the two PGPB resulted in increased flower and fruit production, larger fruit size, and higher concentrations of sugars and ascorbic and folic acid in comparison with fruits of uninoculated plants. These results provide further evidence that rhizospheric microorganisms affect fruit crop quality and show that they do so even under conditions of reduced chemical fertilization and can thus be exploited for sustainable agriculture.

  14. Arbuscular mycorrhizal fungi and plant growth-promoting pseudomonads increases anthocyanin concentration in strawberry fruits (Fragaria x ananassa var. Selva) in conditions of reduced fertilization.

    Science.gov (United States)

    Lingua, Guido; Bona, Elisa; Manassero, Paola; Marsano, Francesco; Todeschini, Valeria; Cantamessa, Simone; Copetta, Andrea; D'Agostino, Giovanni; Gamalero, Elisa; Berta, Graziella

    2013-08-06

    Anthocyanins are a group of common phenolic compounds in plants. They are mainly detected in flowers and fruits, are believed to play different important roles such as in the attraction of animals and seed dispersal, and also in the increase of the antioxidant response in tissues directly or indirectly affected by biotic or abiotic stress factors. As a major group of secondary metabolites in plants commonly consumed as food, they are of importance in both the food industry and human nutrition. It is known that arbuscular mycorrhizal (AM) fungi can influence the plant secondary metabolic pathways such as the synthesis of essential oils in aromatic plants, of secondary metabolites in roots, and increase flavonoid concentration. Plant Growth-Promoting Bacteria (PGPB) are able to increase plant growth, improving plant nutrition and supporting plant development under natural or stressed conditions. Various studies confirmed that a number of bacterial species living on and inside the root system are beneficial for plant growth, yield and crop quality. In this work it is shown that inoculation with AM fungi and/or with selected and tested Pseudomonas strains, under conditions of reduced fertilization, increases anthocyanin concentration in the fruits of strawberry.

  15. Arbuscular Mycorrhizal Fungi and Plant Growth-Promoting Pseudomonads Increases Anthocyanin Concentration in Strawberry Fruits (Fragaria x ananassa var. Selva in Conditions of Reduced Fertilization

    Directory of Open Access Journals (Sweden)

    Elisa Gamalero

    2013-08-01

    Full Text Available Anthocyanins are a group of common phenolic compounds in plants. They are mainly detected in flowers and fruits, are believed to play different important roles such as in the attraction of animals and seed dispersal, and also in the increase of the antioxidant response in tissues directly or indirectly affected by biotic or abiotic stress factors. As a major group of secondary metabolites in plants commonly consumed as food, they are of importance in both the food industry and human nutrition. It is known that arbuscular mycorrhizal (AM fungi can influence the plant secondary metabolic pathways such as the synthesis of essential oils in aromatic plants, of secondary metabolites in roots, and increase flavonoid concentration. Plant Growth-Promoting Bacteria (PGPB are able to increase plant growth, improving plant nutrition and supporting plant development under natural or stressed conditions. Various studies confirmed that a number of bacterial species living on and inside the root system are beneficial for plant growth, yield and crop quality. In this work it is shown that inoculation with AM fungi and/or with selected and tested Pseudomonas strains, under conditions of reduced fertilization, increases anthocyanin concentration in the fruits of strawberry.

  16. Arbuscular Mycorrhizal Fungi and Plant Growth-Promoting Pseudomonads Increases Anthocyanin Concentration in Strawberry Fruits (Fragaria x ananassa var. Selva) in Conditions of Reduced Fertilization

    Science.gov (United States)

    Lingua, Guido; Bona, Elisa; Manassero, Paola; Marsano, Francesco; Todeschini, Valeria; Cantamessa, Simone; Copetta, Andrea; D’Agostino, Giovanni; Gamalero, Elisa; Berta, Graziella

    2013-01-01

    Anthocyanins are a group of common phenolic compounds in plants. They are mainly detected in flowers and fruits, are believed to play different important roles such as in the attraction of animals and seed dispersal, and also in the increase of the antioxidant response in tissues directly or indirectly affected by biotic or abiotic stress factors. As a major group of secondary metabolites in plants commonly consumed as food, they are of importance in both the food industry and human nutrition. It is known that arbuscular mycorrhizal (AM) fungi can influence the plant secondary metabolic pathways such as the synthesis of essential oils in aromatic plants, of secondary metabolites in roots, and increase flavonoid concentration. Plant Growth-Promoting Bacteria (PGPB) are able to increase plant growth, improving plant nutrition and supporting plant development under natural or stressed conditions. Various studies confirmed that a number of bacterial species living on and inside the root system are beneficial for plant growth, yield and crop quality. In this work it is shown that inoculation with AM fungi and/or with selected and tested Pseudomonas strains, under conditions of reduced fertilization, increases anthocyanin concentration in the fruits of strawberry. PMID:23924942

  17. Different growing conditions affect nutrient content, fruit yield and growth in strawberry

    International Nuclear Information System (INIS)

    Demirsory, L.; Demirsoy, H.; Balci, G.

    2011-01-01

    This study aimed to determine the effects of organic and conventional growing on contents of some nutrient elements, nitrogen (N), phosphorous (P), potassium (K), iron (Fe), and manganese (Mn), yield and some growth parameters such as leaf area, petiole length, petiole diameter, crown number, crow n diameter, leaf, root dry weight in 'Sweet Charlie' and 'Camarosa' strawberry cultivars. This study consisted of two strawberry cultivars ('Camarosa' and 'Sweet Charlie'), two growing systems (organic and conventional growing) and two different mulches (black and floating sheet). There was significant difference among treatments in terms of P, K, and Mn content in root and Fe content in leaf and yield and some growth parameters. The best treatment in terms of yield and growth parameters was conventional growing with black plastic in 'Camarosa' while the best treatments were organic growing with floating sheet and black plastic in 'Sweet Charlie' in terms of P, K in root and organic growing with floating sheet in 'Sweet Charlie' in terms of Fe in leaf. (author)

  18. Biologia da mosca‑das‑frutas sul‑americana em frutos de mirtilo, amoreira‑preta, araçazeiro e pitangueira Biology of South American fruit fly in blueberry, blackberry, strawberry guava, and Surinam cherry crops

    Directory of Open Access Journals (Sweden)

    Maicon Bisognin

    2013-02-01

    Full Text Available O objetivo deste trabalho foi descrever a biologia de Anastrepha fraterculus em frutos de mirtilo (Vaccinium ashei, amoreira‑preta (Rubus spp., araçazeiro (Psidium cattleyanum e pitangueira (Eugenia uniflora. O experimento foi realizado em laboratório, em condições controladas de temperatura (25±2ºC, umidade relativa (70±10% e fotófase (12 horas, para determinação dos parâmetros biológicos do inseto nos estágios de desenvolvimento imaturos e adultos. Anastrepha fraterculus completa o ciclo biológico em todos hospedeiros estudados, embora os frutos nativos (pitanga e araçá ofereçam melhores condições para seu desenvolvimento. Os parâmetros biológicos determinados para as fases imaturas foram semelhantes nos quatro hospedeiros. Insetos criados em pitanga e araçá apresentam, na fase adulta, maior período de oviposição, fecundidade e longevidade de fêmeas, em comparação aos criados em mirtilo e amora‑preta. O ritmo diário de oviposição é mais prolongado e uniforme nos insetos criados em araçá e pitanga, o que mostra que A. fraterculus está mais bem adaptada a estas frutas, nativas da região Sul.The objective of this work was to describe the biology of Anastrepha fraterculus in blueberry (Vaccinium ashei, blackberry (Rubus spp., strawberry guava (Psidium cattleyanum and Surinam cherry (Eugenia uniflora fruits. The experiment was carried out in laboratory under controlled conditions of temperature (25±2ºC, relative humidity (70±10%, and photophase (12 hours to determine insect biological parameters in immature and adult development stages. Anastrepha fraterculus finishes its biological cycle in all studied hosts; however, the Brazilian native fruits (strawberry guava and Surinam cherry provide better conditions for development of the insect. Biological parameters determined for immature development stadium were similar in the four hosts. Insects reared in Surinam cherry and strawberry guava showed, in the

  19. Comparative Transcriptome Analysis Reveals the Influence of Abscisic Acid on the Metabolism of Pigments, Ascorbic Acid and Folic Acid during Strawberry Fruit Ripening.

    Directory of Open Access Journals (Sweden)

    Dongdong Li

    Full Text Available A comprehensive investigation of abscisic acid (ABA biosynthesis and its influence on other important phytochemicals is critical for understanding the versatile roles that ABA plays during strawberry fruit ripening. Using RNA-seq technology, we sampled strawberry fruit in response to ABA or nordihydroguaiaretic acid (NDGA; an ABA biosynthesis blocker treatment during ripening and assessed the expression changes of genes involved in the metabolism of pigments, ascorbic acid (AsA and folic acid in the receptacles. The transcriptome analysis identified a lot of genes differentially expressed in response to ABA or NDGA treatment. In particular, genes in the anthocyanin biosynthesis pathway were actively regulated by ABA, with the exception of the gene encoding cinnamate 4-hydroxylase. Chlorophyll degradation was accelerated by ABA mainly owing to the higher expression of gene encoding pheide a oxygenase. The decrease of β-carotene content was accelerated by ABA treatment and delayed by NDGA. A high negative correlation rate was found between ABA and β-carotene content, indicating the importance of the requirement for ABA synthesis during fruit ripening. In addition, evaluation on the folate biosynthetic pathway indicate that ABA might have minor function in this nutrient's biosynthesis process, however, it might be involved in its homeostasis. Surprisingly, though AsA content accumulated during fruit ripening, expressions of genes involved in its biosynthesis in the receptacles were significantly lower in ABA-treated fruits. This transcriptome analysis expands our understanding of ABA's role in phytochemical metabolism during strawberry fruit ripening and the regulatory mechanisms of ABA on these pathways were discussed. Our study provides a wealth of genetic information in the metabolism pathways and may be helpful for molecular manipulation in the future.

  20. Effect of drying methods with the application of vacuum microwaves on the bioactive compounds, color, and antioxidant activity of strawberry fruits.

    Science.gov (United States)

    Wojdyło, Aneta; Figiel, Adam; Oszmiański, Jan

    2009-02-25

    The objective of this study was to evaluate the application of vacuum-microwave drying (240, 360, and 480 W) in the production process of dehydrated strawberry and to compare and contrast the quality of these dehydrated strawberries in terms of their polyphenol compounds, concentration of some heat liable components, and color to that of freeze-dried, convective, and vacuum-dried strawberry. Thus, the effect of vacuum-microwave drying and other drying methods on the antioxidant activity of berries was evaluated. Whole fresh and dried fruits were assessed for phenolics (anthocyanins, flavanols, hydroxycinnamic acids, and flavonols), ascorbic acid, and antioxidant activity (all parameters were calculated on a dry matter basis). Analysis of data shows that ellagic acid and flavanol changes were affected by drying techniques and cultivar. Drying destroyed anthocyanins, flavanols, and ascorbic acid, and there was a significant decrease in antioxidant activity. The most striking result was that conventional and vacuum drying decreased antioxidant activity in both cultivars, whereas contradictory results were found for vacuum-microwave processed strawberry. This study has demonstrated that vacuum-microwave drying, especially at 240 W, can produce high-quality products, with the additional advantage of reduced processing times, compared to other processes such as freeze-drying.

  1. Quantitative changes in proteins responsible for flavonoid and anthocyanin biosynthesis in strawberry fruit at different ripening stages: A targeted quantitative proteomic investigation employing multiple reaction monitoring.

    Science.gov (United States)

    Song, Jun; Du, Lina; Li, Li; Kalt, Wilhelmina; Palmer, Leslie Campbell; Fillmore, Sherry; Zhang, Ying; Zhang, ZhaoQi; Li, XiHong

    2015-06-03

    To better understand the regulation of flavonoid and anthocyanin biosynthesis, a targeted quantitative proteomic investigation employing LC-MS with multiple reaction monitoring was conducted on two strawberry cultivars at three ripening stages. This quantitative proteomic workflow was improved through an OFFGEL electrophoresis to fractionate peptides from total protein digests. A total of 154 peptide transitions from 47 peptides covering 21 proteins and isoforms related to anthocyanin biosynthesis were investigated. The normalized protein abundance, which was measured using isotopically-labeled standards, was significantly changed concurrently with increased anthocyanin content and advanced fruit maturity. The protein abundance of phenylalanine ammonia-lyase; anthocyanidin synthase, chalcone isomerase; flavanone 3-hydroxylase; dihydroflavonol 4-reductase, UDP-glucose:flavonoid-3-O-glucosyltransferase, cytochrome c and cytochrome C oxidase subunit 2, was all significantly increased in fruit of more advanced ripeness. An interaction between cultivar and maturity was also shown with respect to chalcone isomerase. The good correlation between protein abundance and anthocyanin content suggested that a metabolic control point may exist for anthocyanin biosynthesis. This research provides insights into the process of anthocyanin formation in strawberry fruit at the level of protein concentration and reveals possible candidates in the regulation of anthocyanin formation during fruit ripening. To gain insight into the molecular mechanisms contributing to flavonoids and anthocyanin biosynthesis and regulation of strawberry fruit during ripening is challenging due to limited molecular biology tools and established hypothesis. Our targeted proteomic approach employing LC-MS/MS analysis and MRM technique to quantify proteins in relation to flavonoids and anthocyanin biosynthesis and regulation in strawberry fruit during fruit ripening is novel. The identification of peptides

  2. Electrochemical determination of morin in Kiwi and Strawberry fruit samples using vanadium pentoxide nano-flakes.

    Science.gov (United States)

    Sasikumar, Ragu; Govindasamy, Mani; Chen, Shen-Ming; Chieh-Liu, Yu; Ranganathan, Palraj; Rwei, Syang-Peng

    2017-10-15

    Herein, we report the synthesis of Vanadium Pentoxide Nanoflakes (V 2 O 5 NF) using ionic liquid and employed the V 2 O 5 NF in electrochemical determination of Morin (MR) in fruit samples. The V 2 O 5 NF were characterized by Powder X-ray diffraction (PXRD), scanning electron microscope (SEM), energy-dispersive X-ray analyzer (EDX), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). Remarkably, the as-synthesized V 2 O 5 NF exhibited excellent electrochemical behavior and electrochemical ability towards MR. The CV and DPV studies were recognized that the electrochemical performance of V 2 O 5 NF film modified glassy carbon electrode (V 2 O 5 NF/GCE) towards detection of MR is outstanding in comparison with unmodified GCE. The proposed MR sensor shows a wide linear range, high sensitivity, and low limit of detection are 0.05-10.93μm, 1.130μAμM -1 cm -2 , and 9nM respectively. The fabulous analytical parameters of the developed sensor surpassed the previously reported modified electrodes, rendering the potential application of V 2 O 5 NF in environmental, biomedical, and pharmaceutical samples. Copyright © 2017. Published by Elsevier Inc.

  3. Strawberry fruit resistance to simulated handling Resistência física de morangos submetidos ao manuseio

    Directory of Open Access Journals (Sweden)

    Marcos David Ferreira

    2008-01-01

    Full Text Available Harvest operations are currently the main source of mechanical injury of strawberry (Fragaria x ananassa Duch.. Experiments were designed to simulate conditions encountered during commercial handling. Individual fruits were subjected to impact or compression forces with similar energy to determine the sensitivity to mechanical injury. Bruise volume was used as the measurement of injury. Bruise severity increased as a function of impact energy for both impact types. However, dropped fruits had larger bruise volume than fruits submitted to pendulum impactor at the same energy level. Doubling the impact energy (0.040 to 0.083 J increased bruise volume by 7 times (13 to 91 mm³. Fruits dropped from 380 mm (0.075 J showed 71% greater bruise volume than those dropped from either 130 mm (0.025 J or 200 mm (0.040 J. Compressed fruits showed higher bruise volume than other tests. Some cultivars are more susceptible to compression forces than others. 'Sweet Charlie' berries showed bruise volume 40% higher than the others cultivars when subjected to compression. Fruits subjected to impact showed bruise volume lower than the compressed fruits, indicating the possibility to be handled and graded in a packing line.A etapa de colheita é a principal fonte de danos físicos ao morango (Fragaria x ananassa Duch.. Experimentos foram realizados para simular condições encontradas durante manuseio. Frutos foram submetidos individualmente às forças de impacto e compressão em energias similares para determinar sensibilidade dos frutos a danos físicos. Volume da injúria física foi utilizado para mensurar a incidência do dano físico ocorrido. Severidade da lesão aumenta, com incremento da energia, tanto para força de impacto como para compressão. Todavia, frutos submetidos à queda livre demonstraram maiores volumes de danos físicos do que frutos submetidos a danos ocasionados por pendulo no mesmo nível de energia. Dobrando a energia de impacto (0

  4. Effects of hot air and freeze drying methods on antioxidant activity, colour and some nutritional characteristics of strawberry tree (Arbutus unedo L) fruit.

    Science.gov (United States)

    Orak, H H; Aktas, T; Yagar, H; İsbilir, S Selen; Ekinci, N; Sahin, F Hasturk

    2012-08-01

    Antioxidant activity, colour and some nutritional properties of hot air and freeze-dried strawberry tree (Arbutus unedo L.) fruits were investigated. Additionally, the effects of two pre-treatments, namely ethyl oleate and water blanching, were compared in terms of drying characteristics. For determination of antioxidant activities in ethanol extracts, two different analytical methods were used: 1,1-diphenyl-2-picrylhydrazyl scavenging activity and β-carotene bleaching activity. As a result, the ethyl oleate pre-treatment shortened the drying time by hot air method and gave a higher 1,1-diphenyl-2-picrylhydrazyl scavenging activity (82.16 ± 0.34%), total phenolic content (7.62 ± 1.09 µg GAE/g extract), ascorbic acid content (236.93 ± 20.14 mg/100 g), besides hydromethylfurfural was not observed. Freeze-dried fruits exhibited higher ascorbic acid content (368.63 ± 17.16 mg/100 g) than those fresh fruits (231.33 ± 19.51 mg/100 g) and nearly 1,1-diphenyl-2-picrylhydrazyl activity (93.52 ± 0.41 %) to fresh fruits (94.03 ± 1.18%). Colour characteristics, sugar content and mineral contents of fruits were significantly affected by pre-treatments and drying methods (p drying of strawberry tree fruits should bring a valuable and attractive foodstuff to food industry due to the rich nutritional components, antioxidant activity and colour. Another conclusion from this study is that the freeze-drying is the best drying method to keep the nutritional value, antioxidant activity and sensory properties of fruits.

  5. Isolation of genes differentially expressed during development and ripening of Fragaria chiloensis fruit by suppression subtractive hybridization.

    Science.gov (United States)

    Pimentel, Paula; Salvatierra, Ariel; Moya-León, María Alejandra; Herrera, Raúl

    2010-09-15

    Fragaria chiloensis, the native Chilean strawberry, is noted for its good fruit quality characters. However, it is a highly perishable fruit due to its rapid softening. With the aim to screen for genes differentially expressed during development and ripening of strawberry fruit, the subtractive suppressive hybridization (SSH) methodology was employed. Six libraries were generated contrasting transcripts from four different developmental stages. A set of 1807 genes was isolated and characterized. In our EST collection, approximately 90% of partial cDNAs showed significant similarity to proteins with known or unknown function registered in databases. Among them, proteins related to protein fate were identified in a large green fruit library and protein related with cellular transport, cell wall-related proteins, and transcription regulators were identified in a ripe fruit library. Thirteen genes were analyzed by qRT-PCR during development and ripening of the Chilean strawberry fruit. The information generated in this study provides new clues to aid the understanding of the ripening process in F. chiloensis fruit. Copyright 2010 Elsevier GmbH. All rights reserved.

  6. Genome-Wide Analysis of the Expression of WRKY Family Genes in Different Developmental Stages of Wild Strawberry (Fragaria vesca Fruit.

    Directory of Open Access Journals (Sweden)

    Heying Zhou

    Full Text Available WRKY proteins play important regulatory roles in plant developmental processes such as senescence, trichome initiation and embryo morphogenesis. In strawberry, only FaWRKY1 (Fragaria × ananassa has been characterized, leaving numerous WRKY genes to be identified and their function characterized. The publication of the draft genome sequence of the strawberry genome allowed us to conduct a genome-wide search for WRKY proteins in Fragaria vesca, and to compare the identified proteins with their homologs in model plants. Fifty-nine FvWRKY genes were identified and annotated from the F. vesca genome. Detailed analysis, including gene classification, annotation, phylogenetic evaluation, conserved motif determination and expression profiling, based on RNA-seq data, were performed on all members of the family. Additionally, the expression patterns of the WRKY genes in different fruit developmental stages were further investigated using qRT-PCR, to provide a foundation for further comparative genomics and functional studies of this important class of transcriptional regulators in strawberry.

  7. Comparative antihemolytic and radical scavenging activities of strawberry tree (Arbutus unedo L.) leaf and fruit.

    Science.gov (United States)

    Mendes, Lídia; de Freitas, Victor; Baptista, Paula; Carvalho, Márcia

    2011-09-01

    The present study reports the antioxidant properties of Arbutus unedo L. leaf and fruit extracts using different in vitro assays including (i) reducing power, (ii) scavenging effect on DPPH free radicals, and (iii) inhibitory effect on AAPH-induced hemolysis and lipid peroxidation in human erythrocytes. All assays demonstrated antioxidant efficiency for A. unedo L. aqueous extracts, being consistently higher in the leaf. EC(50) values for reducing power and DPPH radical scavenging activities were, respectively, 0.318 ± 0.007 and 0.087 ± 0.007 mg/mL for leaf, and 2.894 ± 0.049 and 0.790 ± 0.016 mg/mL for fruit extracts. Under the oxidative action of AAPH, A. unedo leaf and fruit extracts protected the erythrocyte membrane from hemolysis (IC(50) of 0.062 ± 0.002 and 0.430 ± 0.091 mg/mL, respectively) and decreased the levels of malondialdehyde, a breakdown product of lipid peroxidation (IC(50) of 0.075 ± 0.014 and 0.732 ± 0.452 mg/mL, respectively). In accordance with antioxidant activity, phenolic content was found to be significantly higher in leaf extract. To our knowledge, this is the first time that the antioxidant activity of A. unedo species is evaluated using human biological membranes. Overall, our results suggest that A. unedo leaves are a promising source of natural antioxidants with potential application in diseases mediated by free radicals. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. Potential role of pectate lyase and Ca(2+) in the increase in strawberry fruit firmness induced by short-term treatment with high-pressure CO2.

    Science.gov (United States)

    Wang, Mao Hua; Kim, Jin Gook; Ahn, Sun Eun; Lee, Ah Youn; Bae, Tae Min; Kim, Deu Re; Hwang, Yong Soo

    2014-04-01

    Postharvest treatment with high-pressure CO2 helps to control decay and increase firmness in strawberries. Increases in firmness occurred through modification of calcium binding to cell wall. However, the mechanism(s) involved in Ca(2+) migration to pectic polymers and other physiological events associated with the maintenance of increased firmness are not clearly understood. The focus of this study was to find potential mechanism(s) that are associated with calcium movement, increases in firmness, or maintenance of firmness in strawberry fruit after high-pressure CO2 treatment. An increase in firmness was induced by high-pressure CO2 treatment, but not by high-pressure N2 treatment. This indicates that CO2 stimulates a change in firmness. The increase in firmness induced by high-pressure CO2 seems to involve calcium efflux. Using membrane Ca(2+) -dependent ATPase inhibitors sodium vanadate (250 μM) and erythrosin B (100 μM) delayed both the increase in firmness and calcium binding to wall polymers. Exogenous application of CaCl2 (10 mM) enhanced the firmness increase of fruit slices only when they were exposed to high-pressure CO2 . The activity of pectate lyase was downregulated by CO2 treatment, but β-galactosidase activity was not affected. The increase in strawberry firmness induced by high-pressure CO2 treatment primarily involves the efflux of calcium ions and their binding to wall polymers. These physiological changes are not induced by an anaerobic environment. The downregulation of wall-modifying enzymes, such as pectate lyase, appeared to contribute to the maintenance of firmness that was induced by high-pressure CO2 treatment. © 2014 Institute of Food Technologists®

  9. 'Florida Beauty' strawberry

    Science.gov (United States)

    ‘Florida Beauty’ strawberry (Fragaria x ananassa) originated from a 2012 cross made by the Queensland breeding program between Queensland Australia selection 2010-119 (female parent) and ‘Florida Radiance’ (male parent). Selection 2010-119 was chosen as a parent for its excellent fruit shape and fl...

  10. Influence of growing conditions at different latitudes of Europe on strawberry growth performance, yield and quality

    DEFF Research Database (Denmark)

    Krüger, E.; Josuttis, M.; Nestby, R.

    2012-01-01

    The site effect of five locations from north (Stjørdal, Norway, 63°36'N) to south (Ancona, Italy, 43°31'N) was evaluated in strawberry regarding yield performance, fruit quality, length of fruit developing time from anthesis to harvest start and length of the harvest season. Cv. Elsanta was grown...

  11. A novel 3D imaging system for strawberry phenotyping

    Directory of Open Access Journals (Sweden)

    Joe Q. He

    2017-11-01

    Full Text Available Abstract Background Accurate and quantitative phenotypic data in plant breeding programmes is vital in breeding to assess the performance of genotypes and to make selections. Traditional strawberry phenotyping relies on the human eye to assess most external fruit quality attributes, which is time-consuming and subjective. 3D imaging is a promising high-throughput technique that allows multiple external fruit quality attributes to be measured simultaneously. Results A low cost multi-view stereo (MVS imaging system was developed, which captured data from 360° around a target strawberry fruit. A 3D point cloud of the sample was derived and analysed with custom-developed software to estimate berry height, length, width, volume, calyx size, colour and achene number. Analysis of these traits in 100 fruits showed good concordance with manual assessment methods. Conclusion This study demonstrates the feasibility of an MVS based 3D imaging system for the rapid and quantitative phenotyping of seven agronomically important external strawberry traits. With further improvement, this method could be applied in strawberry breeding programmes as a cost effective phenotyping technique.

  12. A novel 3D imaging system for strawberry phenotyping.

    Science.gov (United States)

    He, Joe Q; Harrison, Richard J; Li, Bo

    2017-01-01

    Accurate and quantitative phenotypic data in plant breeding programmes is vital in breeding to assess the performance of genotypes and to make selections. Traditional strawberry phenotyping relies on the human eye to assess most external fruit quality attributes, which is time-consuming and subjective. 3D imaging is a promising high-throughput technique that allows multiple external fruit quality attributes to be measured simultaneously. A low cost multi-view stereo (MVS) imaging system was developed, which captured data from 360° around a target strawberry fruit. A 3D point cloud of the sample was derived and analysed with custom-developed software to estimate berry height, length, width, volume, calyx size, colour and achene number. Analysis of these traits in 100 fruits showed good concordance with manual assessment methods. This study demonstrates the feasibility of an MVS based 3D imaging system for the rapid and quantitative phenotyping of seven agronomically important external strawberry traits. With further improvement, this method could be applied in strawberry breeding programmes as a cost effective phenotyping technique.

  13. Quantification of Fusarium oxysporum in fumigated soils by a newly developed real-time PCR assay to assess the efficacy of fumigants for Fusarium wilt disease in strawberry plants.

    Science.gov (United States)

    Li, Yuan; Mao, Liangang; Yan, Dongdong; Ma, Taotao; Shen, Jin; Guo, Meixia; Wang, Qiuxia; Ouyang, Canbin; Cao, Aocheng

    2014-11-01

    Two soil fumigants, chloropicrin (CP) and dimethyl disulfide (DMDS), were used to control Fusarium wilt disease (FWD) which caused large economic losses in strawberries. The fumigants were evaluated alone and in combination in a laboratory study and in strawberry greenhouses. Laboratory tests found that combinations of CP and DMDS indicated a positive synergistic activity on Fusarium oxysporum. A newly developed quantitative assay for F. oxysporum involving real-time PCR was used successfully to evaluate F. oxysporum control by the fumigants; it provided similar results to the selective medium but was less time-consuming and less labor intensive. Greenhouse trials revealed that the combination of CP and DMDS successfully suppressed the incidence of FWD and sharply reduced the population density of F. oxysporum, which significantly increased fruit branch number and maintained a good strawberry yield, higher than methyl bromide (MB) treatment. All of the treatments provided significantly better results than the non-treated control. This study confirms that the newly developed real-time PCR quantitative assay for F. oxysporum was suitable for the control efficacy evaluation of soil fumigants and that the novel fumigant combination of CP and DMDS offers a promising effective alternative to MB for the control of F. oxysporum in strawberry greenhouses. © 2013 Society of Chemical Industry.

  14. An Apple Fruit Fermentation (AFF) Treatment Improves the Composition of the Rhizosphere Microbial Community and Growth of Strawberry (Fragaria × ananassa Duch ‘Benihoppe’) Seedlings

    Science.gov (United States)

    Bu, Yufen; Shao, Wei; Huang, Weijing; Ji, Qianlong; Yao, Yuncong

    2016-01-01

    Plant growth can be promoted by the application of apple fruit fermentation (AFF), despite unclear of the underlying mechanisms, the effects involved in AFF on rhizosphere microorganisms have been hypothesized. We investigated the consequences of applying AFF alone or in combination with Bacillus licheniformis to strawberry tissue culture seedlings in vitro, the analyses of Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rDNA were performed to determine AFF effects on rhizosphere. Moreover, the growth index and antioxidant enzyme activities were determined 30 days after treatments. We identified five dominant bacteria in AFF: Coprinus atramentarius, Bacillus megaterium, Bacillus licheniformis, Weissella and B. subtilis. The greatest number of bacterial species were observed in the rhizosphere of control matrix (water treated), and the lowest diversity appeared in the rhizosphere soil treated with 108 cfu/mL B. licheniformis alone. Combining AFF plus B. licheniformis in one treatment resulted in the largest leaf area, plant height, root length, plant weight, and the markedly higher activities of antioxidant enzymes. We conclude that a combination of AFF plus B. licheniformis treatment to matrix can increase antioxidant enzymes activities in strawberry seedlings, optimize the status of rhizosphere microbial, and promote plant growth. PMID:27755580

  15. Effect of Storage on the Physico-Chemical and Antioxidant Properties of Strawberry and Kiwi Leathers.

    Science.gov (United States)

    Concha-Meyer, Anibal A; D'Ignoti, Valeria; Saez, Barbara; Diaz, Ricardo I; Torres, Carolina A

    2016-03-01

    Strawberry and kiwi leathers were used to develop a new healthy and preservative-free fruit snack for new markets. Fruit puree was dehydrated at 60 °C for 20 h and subjected to accelerated storage. Soluble solids, titratable acidity, pH, water activity (aw ), total phenolic (TP), antioxidant activity (AOA) and capacity (ORAC), and color change (browning index) were measured in leathers, cooked, and fresh purees. An untrained panel was used to evaluate consumer acceptability. Soluble solids of fresh purees were 11.24 to 13.04 °Brix, whereas pH was 3.46 to 3.39. Leathers presented an aw of 0.59 to 0.67, and a moisture content of 21 kg water/100 kg. BI decreased in both leathers over accelerated storage period. TP and AOA were higher (P ≤ 0.05) in strawberry formulations. ORAC decreased 57% in strawberry and 65% in kiwi leathers when compared to fruit puree. TP and AOA increased in strawberries during storage. Strawberry and Kiwi leathers may be a feasible new, natural, high antioxidant, and healthy snack for the Chilean and other world markets, such as Europe, particularly the strawberry leather, which was preferred by untrained panelists. © 2016 Institute of Food Technologists®

  16. Radiotracer studies on the formation of 2,5-dimethyl-4-hydroxy-3(2H)-furanone in detached ripening strawberry fruits

    International Nuclear Information System (INIS)

    Roscher, R.; Bringmann, G.; Schreier, P.; Schwab, W.

    1998-01-01

    The transformation of 12 radioactively labeled compounds into 2,5-dimethyl-4-hydroxy-3(2H)-furanone (DMHF), glycosidically bound DMHF, and 2,5-dimethyl-4-methoxy-3(2H)-furanone (DMMF) was investigated in detached ripening strawberry fruits (Fragaria x ananassa) over a 3-day period. Radiochemical analysis of the different fruit parts revealed that major portions of the applied radioactivity (up to 66%) remained in the stems and calyx. Incorporation levels of [2- 14 C]-dihydroxyacetone, D-[1- 3 H]glucose, D-[U- 14 C]-glucose, D-[U- 14 C]glucose 6-phosphate, D-[U- 14 C]fructose, and D-[U- 14 C]fructose 1,6-bisphosphate into the total amount of furanone derivatives were 0.022, 0.032, 0.035, 0.147, 0.202, and 0.289% of the radioactivity entering the fruits, respectively. Minor amounts of radioactivity (0.001%) were detected in the furanone structures after the administration of [1- 14 C]acetate and [3- 14 C]pyruvate. L-[1- 14 C]Fucose, L-[6- 3 H]fucose, L-[1- 3 H]rhamnose, L-[U- 14 C]-threonine, L-[U- 14 C]lactaldehyde, and [2- 14 C]malonic acid were not transformed into DMHF or a derivative thereof. (author)

  17. Effect of irradiation on fresh-keeping of strawberry stored at room temperature

    International Nuclear Information System (INIS)

    Zhao Yongfu; Xie Zongchuan; Lu Zhaoxin

    1999-01-01

    The fresh keeping period of strawberry irradiated with 4.0 kGy dose and stored at room temperature was prolonged to 6 days. Further experiment showed that the irradiation treatment decreased the number of mold in strawberry by two orders of magnitude, inhibited the strawberry fruit respiration and water loss, therefore, improved the effect of strawberry fresh-keeping

  18. Influência do pH da solução extrativa no teor de antocianinas em frutos de morango Influence of the extraction solution pH on the content of anthocyanins in strawberry fruits

    Directory of Open Access Journals (Sweden)

    Celso Luiz Bordignon Jr.

    2009-03-01

    Full Text Available Nos últimos anos, os estudos sobre a composição química do fruto do morangueiro vêm ganhando destaque devido ao seu elevado consumo e às atividades biológicas atribuídas a ele. Além disso, os principais metabólitos secundários encontrados nesses frutos são as antocianinas, compostos responsáveis pela intensa coloração vermelha e amplamente utilizados como corantes naturais pela indústria alimentícia. Neste trabalho, foram preparados diferentes extratos de frutos de morango com a cultivar Oso Grande, com o intuito de verificar a influência do pH no perfil espectroscópico do extrato e no teor de antocianos. O extrato em pH 1,0 forneceu um perfil clássico para antocianinas na análise por espectroscopia no ultravioleta, assim como um maior teor de antocianinas nos frutos frescos devido a uma extração mais eficiente das substâncias de interesse.Recently, the study on the chemical composition of the strawberry fruit has been recognized in view of its increased consumption and biological activities. Moreover, the main secondary metabolites found in strawberry fruits are anthocyanins, which are the compounds responsible for the intense red color of the fruit and are widely used as natural colorants by the food industry. In this work, were prepared different extracts with strawberry fruits of the cultivar Oso Grande in order to verify the pH influence on the spectroscopic profile of the strawberry extracts and on the content of anthocyanins. The results indicate that at pH 1,0 the extract showed a classic profile to anthocyanins through ultraviolet spectra analysis and a higher content of anthocyanins in fresh fruits.

  19. Conservation biological control in strawberry: effect of different pollen on development, survival, and reproduction of Neoseiulus californicus (Acari: Phytoseiidae).

    Science.gov (United States)

    Gugole Ottaviano, María F; Cédola, Claudia V; Sánchez, Norma E; Greco, Nancy M

    2015-12-01

    Wild vegetation surrounding crops may provide temporary habitat and potential food sources for phytoseiids in different seasons. Monthly vegetation samples of wild plants adjacent to strawberry plants and wild plants in a vegetation strip close to the crop were taken. The frequency of Neoseiulus californicus, Tetranychus urticae and other mites and insects was recorded. In addition, in a laboratory assay, the survival, developmental time and fecundity of females fed on pollen of strawberry and pollen of wild plants where N. californicus was recorded during their flowering, were estimated. Pollen from Urtica urens, Lamium amplexicaule, Convolvulus arvensis, Sonchus oleraceous, Galega officinalis, and Fragaria x ananassa (strawberry) allowed development of N. californicus to adult, but not reproduction. Survival was 70-80 % when fed on pollen from S. oleraceus, G. officinalis and C. arvensis, 80-90 % when fed on pollen from U. urens and F. x ananassa, and more than 90 % when fed on T. urticae and on pollen from L. amplexicaule. In autumn and winter, U. urens, L. amplexicaule and S. oleraceous could promote the persistence of N. californicus when prey density in strawberry is low, offering T. urticae, thrips and pollen. In summer, pollen of C. arvensis and G. officinalis would contribute to the persistence of N. californicus when the strawberry crop is ending and offers scarce food resources. Although the pollen of these plants would not enable the predator population to increase, the presence of these plants in the vicinity of strawberry could contribute to the persistence of N. californicus population and help to limit T. urticae growth when this pest begins to colonize the crop.

  20. Proximate analysis, in vitro organic matter digestibility, and energy content of common guava (Psidium guajava L.) and yellow, strawberry guava (Psidium cattleianum Var. lucidum) tree parts and fruits as potential forage.

    Science.gov (United States)

    Adrian, Julie Ann Luiz; Arancon, Norman Q; Mathews, Bruce W; Carpenter, James R

    2012-10-24

    The nutrient composition of common guava, Psidium guajava L., and strawberry guava (waiwi), Psidium cattleianum var. lucidum, tree parts and fruits was determined during three seasons for six locations in Hawaii to assess guava as a potential feed for cattle. All guava plant parts were higher (p Guava leaves were higher in fiber and had lower energy densities (p Guava fruits were higher in CP (p guava is low in vitro organic matter digestibility as compared to tropical forage grasses; therefore, it is not recommended as a feedstock for livestock.

  1. Rosaceae Fruit Development, Ripening and Post-harvest: An Epigenetic Perspective.

    Science.gov (United States)

    Farinati, Silvia; Rasori, Angela; Varotto, Serena; Bonghi, Claudio

    2017-01-01

    Rosaceae is a family with an extraordinary spectrum of fruit types, including fleshy peach, apple, and strawberry that provide unique contributions to a healthy diet for consumers, and represent an excellent model for studying fruit patterning and development. In recent years, many efforts have been made to unravel regulatory mechanism underlying the hormonal, transcriptomic, proteomic and metabolomic changes occurring during Rosaceae fruit development. More recently, several studies on fleshy (tomato) and dry (Arabidopsis) fruit model have contributed to a better understanding of epigenetic mechanisms underlying important heritable crop traits, such as ripening and stress response. In this context and summing up the results obtained so far, this review aims to collect the available information on epigenetic mechanisms that may provide an additional level in gene transcription regulation, thus influencing and driving the entire Rosaceae fruit developmental process. The whole body of information suggests that Rosaceae fruit could become also a model for studying the epigenetic basis of economically important phenotypes, allowing for their more efficient exploitation in plant breeding.

  2. Proteome Regulation during Olea europaea Fruit Development

    DEFF Research Database (Denmark)

    Bianco, Linda; Alagna, Fiammetta; Baldoni, Luciana

    2013-01-01

    Background: Widespread in the Mediterranean basin, Olea europaea trees are gaining worldwide popularity for the nutritional and cancer-protective properties of the oil, mechanically extracted from ripe fruits. Fruit development is a physiological process with remarkable impact on the modulation...

  3. Phenylpropenes: Occurrence, Distribution, and Biosynthesis in Fruit.

    Science.gov (United States)

    Atkinson, Ross G

    2018-03-14

    Phenylpropenes such as eugenol, chavicol, estragole, and anethole contribute to the flavor and aroma of a number of important herbs and spices. They have been shown to function as floral attractants for pollinators and to have antifungal and antimicrobial activities. Phenylpropenes are also detected as free volatiles and sequestered glycosides in a range of economically important fresh fruit species including apple, strawberry, tomato, and grape. Although they contribute a relatively small percentage of total volatiles compared with esters, aldehydes, and alcohols, phenylpropenes have been shown to contribute spicy anise- and clove-like notes to fruit. Phenylpropenes are typically found in fruit throughout development and to reach maximum concentrations in ripe fruit. Genes involved in the biosynthesis of phenylpropenes have been characterized and manipulated in strawberry and apple, which has validated the importance of these compounds to fruit aroma and may help elucidate other functions for phenylpropenes in fruit.

  4. Determination of free amino acids and 18 elements in freeze-dried strawberry and blueberry fruit using an Amino Acid Analyzer and ICP-MS with micro-wave digestion.

    Science.gov (United States)

    Zhang, Hua; Wang, Zhen-Yu; Yang, Xin; Zhao, Hai-Tian; Zhang, Ying-Chun; Dong, Ai-Jun; Jing, Jing; Wang, Jing

    2014-03-15

    The objective of this study was to investigate the level of 18 trace elements of two freeze-dried samples from the Blueberry (Vaccinium corymbosum) and the Strawberry (Fragaria × Ananassa). The total free amino acid composition in the blueberry and strawberry was determined by an Amino Acid Analyzer. Eleven free amino acids were found in both berries. The trace elements in each dried fruit sample were determined by ICP-MS with microwave digestion. The linearity range of the standard curves was 0-1250.0 μg L(-1) (Mg, P, K, Ca),while in all cases, except for B, Na, Al, Cr, Mn, Fe, Ni, Cu, Zn, Se, Cd, Pb, Ge and As, which was 125.0 μg mL(-1), all related coefficients were above 0.9999; recovery was in the range of 79.0-106.8%. Minor concentrations of nutritional elements were found in each freeze-dried berry. In sum, the toxic trace element analysis found the content of toxic trace elements in each freeze-dried berry sample was safe for human consumption and that the overall quality of the blueberry surpassed that of the strawberry. The results certify that the two freeze-dried berries have potential for human consumption in value-added products and have a certain theoretical and practical significance. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

  5. Proteome regulation during Olea europaea fruit development.

    Directory of Open Access Journals (Sweden)

    Linda Bianco

    Full Text Available Widespread in the Mediterranean basin, Olea europaea trees are gaining worldwide popularity for the nutritional and cancer-protective properties of the oil, mechanically extracted from ripe fruits. Fruit development is a physiological process with remarkable impact on the modulation of the biosynthesis of compounds affecting the quality of the drupes as well as the final composition of the olive oil. Proteomics offers the possibility to dig deeper into the major changes during fruit development, including the important phase of ripening, and to classify temporal patterns of protein accumulation occurring during these complex physiological processes.In this work, we started monitoring the proteome variations associated with olive fruit development by using comparative proteomics coupled to mass spectrometry. Proteins extracted from drupes at three different developmental stages were separated on 2-DE and subjected to image analysis. 247 protein spots were revealed as differentially accumulated. Proteins were identified from a total of 121 spots and discussed in relation to olive drupe metabolic changes occurring during fruit development. In order to evaluate if changes observed at the protein level were consistent with changes of mRNAs, proteomic data produced in the present work were compared with transcriptomic data elaborated during previous studies.This study identifies a number of proteins responsible for quality traits of cv. Coratina, with particular regard to proteins associated to the metabolism of fatty acids, phenolic and aroma compounds. Proteins involved in fruit photosynthesis have been also identified and their pivotal contribution in oleogenesis has been discussed. To date, this study represents the first characterization of the olive fruit proteome during development, providing new insights into fruit metabolism and oil accumulation process.

  6. Proteome regulation during Olea europaea fruit development.

    Science.gov (United States)

    Bianco, Linda; Alagna, Fiammetta; Baldoni, Luciana; Finnie, Christine; Svensson, Birte; Perrotta, Gaetano

    2013-01-01

    Widespread in the Mediterranean basin, Olea europaea trees are gaining worldwide popularity for the nutritional and cancer-protective properties of the oil, mechanically extracted from ripe fruits. Fruit development is a physiological process with remarkable impact on the modulation of the biosynthesis of compounds affecting the quality of the drupes as well as the final composition of the olive oil. Proteomics offers the possibility to dig deeper into the major changes during fruit development, including the important phase of ripening, and to classify temporal patterns of protein accumulation occurring during these complex physiological processes. In this work, we started monitoring the proteome variations associated with olive fruit development by using comparative proteomics coupled to mass spectrometry. Proteins extracted from drupes at three different developmental stages were separated on 2-DE and subjected to image analysis. 247 protein spots were revealed as differentially accumulated. Proteins were identified from a total of 121 spots and discussed in relation to olive drupe metabolic changes occurring during fruit development. In order to evaluate if changes observed at the protein level were consistent with changes of mRNAs, proteomic data produced in the present work were compared with transcriptomic data elaborated during previous studies. This study identifies a number of proteins responsible for quality traits of cv. Coratina, with particular regard to proteins associated to the metabolism of fatty acids, phenolic and aroma compounds. Proteins involved in fruit photosynthesis have been also identified and their pivotal contribution in oleogenesis has been discussed. To date, this study represents the first characterization of the olive fruit proteome during development, providing new insights into fruit metabolism and oil accumulation process.

  7. Detecting irradiation of strawberries by means of thermoluminescence measurement

    International Nuclear Information System (INIS)

    Heide, L.; Guggenberger, R.; Boegl, K.W.

    1989-01-01

    Investigations into the origin of the thermoluminescence (TL) have shown that this effect is due to trace amounts of mineral particulates adhering to the irradiated material. This result and own experience have led to the development of the TL-based method for post-factum detection of an irradiation of strawberries. In addition to the analysis of whole fruits, a method has been tested that proceeds via separation of adhering matter to separate TL analysis of the particulates. The results obtained so far show no difference or improvement as compared to whole-fruit TL analysis. (orig./MG) [de

  8. The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area

    NARCIS (Netherlands)

    Zuidmeer, L.; Salentijn, E.; Rivas, M. F.; Mancebo, E. G.; Asero, R.; Matos, C. I.; Pelgrom, K. T. B.; Gilissen, L. J. W. J.; van Ree, R.

    2006-01-01

    BACKGROUND: In contrast to other Rosaceae fruit, only few cases of patients with adverse reactions to strawberry are listed in literature. OBJECTIVE To identify allergenic proteins in strawberry and to express and characterize recombinant strawberry lipid transfer protein (LTP; rFra a 3). METHODS:

  9. Coating effect of chitosan irradiation on performance of strawberries (Fragaria x ananassa Duchesne)

    International Nuclear Information System (INIS)

    Gatot Trimulyadi Rekso; Adjat Sudradjat

    2016-01-01

    Strawberry is a horticultural product which has high economic value, however, strawberries are easily damaged. Therefore, required proper post harvest handling for keep up the appearances of strawberries, one of them is the edible coating technique using chitosan. The purpose of this research was to maintaining the appearance of strawberry. Chitosan is irradiated at a dose of 0 kGy, 5 kGy, and 10 kGy, and then characterized. Observations were carried on for five days at room temperature including: the testing of the performance of the fruit that was observed visually and analysis of fruit weight loss. The results showed that the performance of strawberries is coating with irradiated chitosan of 10 kGy is still good. The percentage of weight loss strawberries coated with irradiated chitosan smaller than the strawberries without coating with chitosan (control). Strawberries coated with irradiated chitosan has a shelf life and weight loss better than control. (author)

  10. Synteny conservation between two distantly-related Rosaceae genomes: Prunus (the stone fruits and Fragaria (the strawberry

    Directory of Open Access Journals (Sweden)

    Sargent Daniel J

    2008-06-01

    Full Text Available Abstract Background The Rosaceae encompass a large number of economically-important diploid and polyploid fruit and ornamental species in many different genera. The basic chromosome numbers of these genera are x = 7, 8 and 9 and all have compact and relatively similar genome sizes. Comparative mapping between distantly-related genera has been performed to a limited extent in the Rosaceae including a comparison between Malus (subfamily Maloideae and Prunus (subfamily Prunoideae; however no data has been published to date comparing Malus or Prunus to a member of the subfamily Rosoideae. In this paper we compare the genome of Fragaria, a member of the Rosoideae, to Prunus, a member of the Prunoideae. Results The diploid genomes of Prunus (2n = 2x = 16 and Fragaria (2n = 2x = 14 were compared through the mapping of 71 anchor markers – 40 restriction fragment length polymorphisms (RFLPs, 29 indels or single nucleotide polymorphisms (SNPs derived from expressed sequence tags (ESTs and two simple-sequence repeats (SSRs – on the reference maps of both genera. These markers provided good coverage of the Prunus (78% and Fragaria (78% genomes, with maximum gaps and average densities of 22 cM and 7.3 cM/marker in Prunus and 32 cM and 8.0 cM/marker in Fragaria. Conclusion Our results indicate a clear pattern of synteny, with most markers of each chromosome of one of these species mapping to one or two chromosomes of the other. A large number of rearrangements (36, most of which produced by inversions (27 and the rest (9 by translocations or fission/fusion events could also be inferred. We have provided the first framework for the comparison of the position of genes or DNA sequences of these two economically valuable and yet distantly-related genera of the Rosaceae.

  11. Heat shock and salicylic acid on postharvest preservation of organic strawberries

    Directory of Open Access Journals (Sweden)

    Sidiane Coltro

    2014-06-01

    Full Text Available Heat shock and salicylic acid have been studied on shelf-life extension of fruits. The benefits of these techniques have been related to their effect on inducing physiological defense responses against the oxidative stress and pathogen development. The objective of this study was to evaluate the effect of heat shock and salicylic acid on the postharvest preservation and contents of total phenolics, anthocyanins, ascorbic acid, fresh weight loss and microbiological quality of organic strawberries cv. Dover. Strawberries produced organically and stored at 5 ºC were subjected to heat shock (45 ºC ± 3 ºC for 3 h, application of salicylic acid (soaking in 2.0 mmol L-1 solution, heat shock in combination with salicylic acid and control. After treatment, the fruits were packed and stored in a climatic chamber at 5 ºC ± 2 ºC. At 1, 7 and 14 days, the experimental units were removed from refrigeration and kept at room temperature of approximately 20 ºC for two days. There was no effect of treatments on fresh weight loss, incidence of pathogens or chemical variations in strawberry fruits during the storage period. In natural conditions, organically grown strawberries remained in good condition for sale up to seven days of storage in all treatments.

  12. Breeding new improved clones for strawberry production in Brazil

    Directory of Open Access Journals (Sweden)

    Alexandre Gonçalves Galvão

    2017-04-01

    Full Text Available Breeding different strawberry genotypes and plant selection in Brazil could result in new cultivars with better environmental adaptations. The aim was to develop and select new F1 strawberry plants with higher potential yields. Twelve hybrid populations were obtained from breeding the cultivars Aromas, Camarosa, Dover, Festival, Oso Grande, Sweet Charlie and Tudla, and 42 F1 hybrids were obtained from each population. An augmented randomized block design was used. Productive traits were measured and heterosis was calculated for all traits. The breedings Dover x Aromas and Camarosa x Aromas both showed 28.6% of their hybrids with a total fruit mass that was higher than that of cv. Aromas, and 9.5 and 14.3% were higher than that of cv. Camarosa, respectively. The breeding of Camarosa x Aromas produced hybrids with high potential yields and a large average fruit mass that reached the commercial standard. Hybrids MCA12-93, MFA12-443 and MCA12-89 showed high potential yields and can be used as parents in strawberry breeding programs.

  13. Excellent fruits of national research and development

    International Nuclear Information System (INIS)

    2008-12-01

    This book introduces 100 excellent fruits of research and development in Korea. Such as. It show the titles of 100 excellent fruits about technology of hope to give people healthy life, technology of product and abundance to enrich resource, technology to make green growth and sound society, next service technology to communicate with network, new electron technology to brings life change, technology to draw innovation of industrial fields, technology to build higher value-added product with new fusion materials and technology to share and to create future knowledge.

  14. Development of Cladium mariscus (L. Pohl. fruit

    Directory of Open Access Journals (Sweden)

    Stanisław Marek

    2014-01-01

    Full Text Available The ovule and ovary development was investigated with the aim to establish from what is the scleroid cover of the Cladium mariscus fruit derived. It was proved, that like in other members of the Cyperaceae family, it derives from the ovary.

  15. Proteomics in the fruit tree science arena: new insights into fruit defense, development, and ripening.

    Science.gov (United States)

    Molassiotis, Athanassios; Tanou, Georgia; Filippou, Panagiota; Fotopoulos, Vasileios

    2013-06-01

    Fruit tree crops are agricultural commodities of high economic importance, while fruits also represent one of the most vital components of the human diet. Therefore, a great effort has been made to understand the molecular mechanisms covering fundamental biological processes in fruit tree physiology and fruit biology. Thanks to the development of cutting-edge "omics" technologies such as proteomic analysis, scientists now have powerful tools to support traditional fruit tree research. Such proteomic analyses are establishing high-density 2DE reference maps and peptide mass fingerprint databases that can lead fruit science into a new postgenomic research era. Here, an overview of the application of proteomics in key aspects of fruit tree physiology as well as in fruit biology, including defense responses to abiotic and biotic stress factors, is presented. A panoramic view of ripening-related proteins is also discussed, as an example of proteomic application in fruit science.

  16. Sensory analysis and chemical characterization of strawberry fruits Análise sensorial e caracterização química de frutos de morango

    Directory of Open Access Journals (Sweden)

    Juliano Tadeu V de Resende

    2008-09-01

    Full Text Available The aim of this work was to carry out the chemical characterization and sensory analysis of strawberry fruits from cultivars Camp-dover, Dover, Camp-oso, Oso Grande, and Tudla-Milsei, grown in the southern region of Minas Gerais State. Soluble solids, titratable acidity, and the ratio between both characteristics were evaluated, in three replications. Sensory analysis was carried out by a panel of 34 non-trained assessors, recruited out of UNICENTRO students, professors, and employees. Assessors evaluated the samples for aroma and flavor using sorting-preference tests, with scores from 1 (less preferred to 5 (most preferred. Appearance was assessed by means of a hedonic scale, from 1 (dislike very much to 9 (like very much. Each assessor evaluated five samples of each cultivar for aroma and flavor. To evaluate external appearance, fruits were presented in polystyrene trays, covered with transparent film. Cultivars Camp-dover, Oso Grande, and Tudla-Milsei had the highest (pO trabalho teve como objetivo realizar a caracterização química e a análise sensorial de morangos das cultivares Camp-dover, Dover, Camp-oso, Oso Grande e Tudla-Milsei, produzidas na região Sul de Minas Gerais. Foram avaliados teor de sólidos solúveis e acidez titulável, além da razão entre as duas características, em três repetições. A análise sensorial foi realizada por um painel de 34 avaliadores, formado por alunos, professores e funcionários da UNICENTRO. O painel avaliou o aroma e o sabor dos frutos utilizando testes de ordenação-preferência, com notas variando de 1 (menor preferência a 5 (maior preferência. A aparência foi avaliada por meio de escala hedônica, de 1 (desgostei muito a 9 (gostei muito. Cada painelista avaliou cinco amostras de cada cultivar para aroma e sabor. Para avaliação da aparência, os frutos foram apresentados em bandejas plásticas, cobertas com filme plástico transparente. As cultivares Camp-Dover, Oso Grande e Tudla

  17. Signum, a new fungicide with interesting properties in resistance management of fungal diseases in strawberries.

    Science.gov (United States)

    Hauke, K; Creemers, P; Brugmans, W; Van Laer, S

    2004-01-01

    Signum, a new fungicide developed by BASF, was applied during 6 successive years against fungal diseases in strawberries. The product is formulated as a water dispersible granule, containing 6.7 % pyraclostrobin and 26.7 % boscalid. Pyraclostrobin is similar in chemistry to other strobilurin fungicides like kresoxim-methyl and trifloxystrobin, registered for fruit disease control. Boscalid belongs to the class of carboxyanilides. Both components in the premix formulation combine two different biochemical modes of action in the fungal cell respiration. Therefore, this co-formulation gives a broad-spectrum activity and also a reduced resistance risk for different target pathogens. Botrytis cinerea is the most important disease on strawberry-fruits and thus the control of fruit rot is mainly focused on this fungus. In average over 6 years, Signum has not only given a very good control against Botrytis fruit rot, but it has also shown a high performance in the control of Colletotrichum. Besides, Signum provides good control of powdery mildew (Podosphaera aphanis) and limits the shift to other fruit rots like leather rot (Phytophthora cactorum and leak (Rhizopus, Mucor). The availability of several categories of fungicide families with a different mode of action gives opportunities in alternating different fungicides and is the best guarantee for a sustainable control of fruit rot in all kinds of strawberry production methods. Signum should be integrated in an overall disease management program. Trials, in which the applications of Signum were timed on disease forecasting, based on environmental factors favorable for Botrytis development, were very promising. This tool can also help in establishing the IPM-concept in the production of strawberries.

  18. The Effect of Potassium on Growth and Yield of Strawberry (Fragaria Ananassa (Duchesne ex Weston) Duchesne ex Rozier)

    International Nuclear Information System (INIS)

    Bibi, S; Rhaman, I.; Ijaz, F.; Afzal, A.; Khan, M.; Khan, R.

    2016-01-01

    Fragaria ananassa (Duchesne ex Weston) Duchesne ex-Rozier (Strawberry) variety chandler of Agriculture Research Station (ARS) Baffa, Mansehra was evaluated for different growth and yield parameters under five treatments. In this experiment Potassium was used in 5 different treatments of 3 replications (T0 = Control, T1 = 200 g/6.75m/sup 2/, T2 = 400 g/6.75m/sup 2/, T3 = 600 g/6.75m/sup 2/, T4 = 800 g/6.75m/sup 2/). The results showed maximum value under T3 (405g/6.75m/sup 2/) for characteristics like survival percentage (83.54 percent), plant height (32.05 cm), canopy size (13.62 cm), crown diameter (2.73 cm), number of branches (35.83), number of leaves (67.00), leaf length (5.87 cm), root length (3.51 cm), total number of fruits (43.93), days to 50 percent fruiting (233.67), fruit diameter (3.96 cm), fruit weight (411.40g). Moreover, transplanting date (20 Sep, 2013) is suitable to get maximum yield of strawberry variety Chandler. The findings of this experiment showed that great variations in morphological, physical and agronomic traits of strawberry variety and can be utilized to develop high yield and better quality of strawberry. (author)

  19. Floração, frutificação e maturação de frutos de morangueiro cultivados em ambiente protegido Blooming, fruit set, and fruit maturation of strawberry growing in protected environment

    Directory of Open Access Journals (Sweden)

    Odirce Teixeira Antunes

    2006-12-01

    Full Text Available Determinou-se o número de dias para a ocorrência e a duração dos estádios fenológicos de morangueiro, bem como o período de floração, frutificação e maturação dos frutos das cvs. Oso Grande, Tudla, Chandler e Dover. O trabalho foi executado no interior de uma estrutura galvanizada de 510 m² (estufa plástica, em Passo Fundo-RS, em 2003 e 2004. A primeira etapa constou da avaliação das datas de ocorrência e da duração dos estádios fenológicos das quatro cultivares de morangueiro, em um experimento fatorial (cultivares x estádios fenológicos com delineamento inteiramente casualizados, em cinco repetições. Na segunda etapa, determinou-se o número de dias até o início da floração (50% da área em floração e da formação de frutos (10% dos frutos maiores que 2,5 cm de comprimento, início e final da colheita. Os nove estádios fenológicos foram determinados pela seguinte escala: 1= aparecimento do botão floral; 2= aparecimento das pétalas; 3= flores completamente abertas; 4= pétalas secas e caídas; 5= formação do fruto; 6= aumento do tamanho do fruto; 7= fruto com sementes visíveis no receptáculo; 8= começo da maturação, com maioria dos frutos brancos; 9= frutos maduros com 75 a 100% da superfície vermelha. A duração dos nove estágios variou de 36,4 dias para a cv, Tudla a 40 dias para a cv. Oso Grande. O estádio 4, caracterizado pela queda das pétalas foi o mais longo para todas as cultivares, com 11,3 dias, em média. Para a cultivar Dover, além dessa fase, também o estádio 6 foi o mais prolongado. Considerando o início do florescimento e da frutificação, a cultivar Dover foi a mais precoce, quando comparada com as demais cultivares.The duration time of phenological stages, blooming, fruit set, and maturation periods of strawberry cultivars Oso Grande, Tudla, Chandler, and Dover were evaluated. The study was carried out in a 510 m2 plastic greenhouse, in Passo Fundo- RS, Brazil, in 2003 and

  20. Evaluation Of Growth And Development In Mango Fruits Cvs. Julie ...

    African Journals Online (AJOL)

    Evaluation Of Growth And Development In Mango Fruits Cvs. Julie And Peter To Determine Maturity. ... Two mango cultivars viz., Julie and Peter, were studied for growth and development of fruits in 2001 and 2002 ... HOW TO USE AJOL.

  1. development of motorized development of motorized oil palm fruit

    African Journals Online (AJOL)

    eobe

    industrial activity in a number of developing West. African and ... pharmaceutical industries for the manufacture of drugs. ... production line of which oil palm fruit digestion is one. Thus, one ... For effective design of the machine, it was necessary.

  2. Development of bioactive coatings based on γ-irradiated proteins to preserve strawberries

    International Nuclear Information System (INIS)

    Vu, K.D.; Hollingsworth, R.G.; Salmieri, S.; Takala, P.N.; Lacroix, M.

    2012-01-01

    Gamma irradiation was applied for creating cross-linked proteins to enhance the physicochemical properties of edible films made of calcium caseinate, whey protein isolate and glycerol. The characteristics of γ irradiated cross-linked proteins were analyzed by Fourier Transform Infrared spectroscopy. A second derivative spectra exhibited changes in band intensities that were correlated to an increase of β-sheet structure and a decrease of α-helix and unordered fractions of γ irradiated-cross-linked proteins as compared to the control without irradiation. Furthermore, on addition of methylcellulose to the irradiated protein matrix it was found that it has potential in enhancing the puncture strength and has no detrimental effect on water vapor permeability of protein based films. Finally, these film formulations were used as bioactive edible coatings containing natural antimicrobial agents (limonene and peppermint) to preserve the shelf life of fresh strawberries during storage. The bioactive coatings containing peppermint was found to be more efficient as preserving coatings than the formulations containing limonene. Irradiated proteins/methylcellulose/peppermint formulation had only 40% of decay at day 8 while it was 65% for the control. - Highlights: ► Crosslinked proteins and antimicrobials agents was able to preserve strawberries. ► Crosslinked protein structure was more ordered. ► Films based on crosslinked proteins and methylcellulose enhanced puncture strength.

  3. Implementasi Inovasi Budidaya Stroberi di Agrowisata Banyuroto Kabupaten Magelang Melalui Education for Sustainable Development

    Directory of Open Access Journals (Sweden)

    Ganies Riza Aristya

    2017-07-01

    Full Text Available The cultivation of strawberry needs of technological innovation to gain competitive advantage strawberry fruit quality and quantity. This is motivated because the decline in the production of strawberries in the area and quality of strawberry fruit is still low. Since 2012 until now, Laboratory of Genetics has identified and developed the character of phenotype and genotype of strawberry plants in Agro Banyuroto, Village Banyuroto Sawangan District of Magelang regency. Character genotypes studied through the identification of ploidy with cytogenetic approach and apply poliploidisasi technology that aims to double the number of sets of chromosomes in order to obtain a plant that has the character of superior phenotype compared with the control. The purpose of this activity was to awaken the capacity of communities that were able to develop and utilize natural resources to meet human needs while maintaining the environmental implementing action plans that lead to sustainable development in a sustainable manner. The method was executed in this activity was the cultivation of strawberries by exploiting natural resources Banyuroto village environment that was using bamboo, strawberry cultivation techniques with vertikultur systems. Moreover, the carrying capacity was also supported by the promotion of village tourism as central region Banyuroto strawberry cultivation of strawberries superior to tourists every week, promotion through Online system (in the network by utilizing Information Technology and Computing and community empowerment with the diversification of food made from strawberries. The end result of this activity was obtained hallmarks of crops polyploidy were plant growth faster, the ability of the formation of stolons and nursery faster, leaf area and stem diameter was wider, the stem stout and roots were longer, the fruit produced was greater and a sweeter taste with more red fruit color. Innovation strawberry crop is expected to be

  4. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit

    Directory of Open Access Journals (Sweden)

    McArtney Steve

    2008-02-01

    Full Text Available Abstract Background Apple fruit develop over a period of 150 days from anthesis to fully ripe. An array representing approximately 13000 genes (15726 oligonucleotides of 45–55 bases designed from apple ESTs has been used to study gene expression over eight time points during fruit development. This analysis of gene expression lays the groundwork for a molecular understanding of fruit growth and development in apple. Results Using ANOVA analysis of the microarray data, 1955 genes showed significant changes in expression over this time course. Expression of genes is coordinated with four major patterns of expression observed: high in floral buds; high during cell division; high when starch levels and cell expansion rates peak; and high during ripening. Functional analysis associated cell cycle genes with early fruit development and three core cell cycle genes are significantly up-regulated in the early stages of fruit development. Starch metabolic genes were associated with changes in starch levels during fruit development. Comparison with microarrays of ethylene-treated apple fruit identified a group of ethylene induced genes also induced in normal fruit ripening. Comparison with fruit development microarrays in tomato has been used to identify 16 genes for which expression patterns are similar in apple and tomato and these genes may play fundamental roles in fruit development. The early phase of cell division and tissue specification that occurs in the first 35 days after pollination has been associated with up-regulation of a cluster of genes that includes core cell cycle genes. Conclusion Gene expression in apple fruit is coordinated with specific developmental stages. The array results are reproducible and comparisons with experiments in other species has been used to identify genes that may play a fundamental role in fruit development.

  5. Temperature affects long-term productivity and quality attributes of day-neutral strawberry for a space life-support system

    Science.gov (United States)

    Massa, Gioia D.; Chase, Elaine; Santini, Judith B.; Mitchell, Cary A.

    2015-04-01

    Strawberry (Fragaria x ananassa L.) is a promising candidate crop for space life-support systems with desirable sensory quality and health attributes. Day-neutral cultivars such as 'Seascape' are adaptable to a range of photoperiods, including short days that would save considerable energy for crop lighting without reductions in productivity or yield. Since photoperiod and temperature interact to affect strawberry growth and development, several diurnal temperature regimes were tested under a short photoperiod of 10 h per day for effects on yield and quality attributes of 'Seascape' strawberry during production cycles longer than 270 days. The coolest day/night temperature regime, 16°/8 °C, tended to produce smaller numbers of larger fruit than did the intermediate temperature range of 18°/10 °C or the warmest regime, 20°/12 °C, both of which produced similar larger numbers of smaller fruit. The intermediate temperature regime produced the highest total fresh mass of berries over an entire production cycle. Independent experiments examined either organoleptic or physicochemical quality attributes. Organoleptic evaluation indicated that fruit grown under the coolest temperature regime tended to score the highest for both hedonic preference and descriptive evaluation of sensory attributes related to sweetness, texture, aftertaste, and overall approval. The physicochemical quality attributes Brix, pH, and sugar/acid ratio were highest for fruits harvested from the coolest temperature regime and lower for those from the warmer temperature regimes. The cool-regime fruits also were lowest in titratable acidity. The yield parameters fruit number and size oscillated over the course of a production cycle, with a gradual decline in fruit size under all three temperature regimes. Brix and titratable acidity both decreased over time for all three temperature treatments, but sugar/acid ratio remained highest for the cool temperature regime over the entire production

  6. Produtividade e teor de sólidos solúveis de frutos de cultivares de morangueiro em ambiente protegido Yield and soluble solids contents of fruits of strawberry cultivars in protected cultivation

    Directory of Open Access Journals (Sweden)

    Juliano Tadeu V de Resende

    2010-06-01

    Full Text Available O cultivo protegido é uma alternativa para controlar variações climáticas em diferentes locais e épocas de plantio, entretanto, seus efeitos e interações com a cultura do morangueiro ainda são pouco conhecidos. O objetivo desse trabalho foi avaliar a influência de três sistemas de cultivo (túnel alto, túnel baixo e a campo na produtividade, massa média de frutos e no teor de sólidos solúveis dos frutos das cultivares de morango Dover, Camarosa, Sweet Charlie e Oso Grande em Guarapuava-PR. Quanto à produtividade e massa média de frutos, a cultivar Camarosa foi a melhor nos cultivos em túnel, tanto alto quanto baixo, pelo seu excelente desempenho. Os frutos da cultivar Sweet Charlie apresentaram o maior teor de sólidos solúveis nos três ambientes de cultivo. A produtividade e a massa média dos frutos foram significativamente superiores nos cultivos em túneis, alto e baixo, quando comparados ao cultivo a campo. O ambiente que propiciou maior acúmulo de sólidos solúveis nos frutos foi o túnel alto, diferindo significativamente dos demais ambientes de cultivo.Protected cultivation is an alternative to control the climatic effects in different places and times of planting, however, its effects and interactions with the strawberry crop are little known. The goal of this study was to evaluate the effects of three cropping systems (high tunnel, low tunnel and field on yield, mean fruit mass and the soluble solids contents of strawberry cultivars Dover, Camarosa, Sweet Charlie and Oso Grande in Guarapuava. Camarosa cultivar presents an excellent performance regarding yield and mean fruit mass in high and low tunnel cultivation; hence, these cropping system can be indicated. Fruits of Sweet Charlie cultivar had the highest content of soluble solids in all the cultivation environments. Yield and mean fruit mass obtained from high and low tunnels were significantly higher than in the field. The environment high tunnel allowed

  7. Strawberry cultivation in Brazil | Cultivo de morangos no Brasil

    Directory of Open Access Journals (Sweden)

    José Machado

    2016-06-01

    Full Text Available The strawberry is cultivated in different regions of the world. Their cultivation have shown demand larger family hand labor and highly profitable.  The strawberry is a very old fruit. Wild species existed for more 50 million years, but the specie was tamed around the XIV century A.C.  There are various types of cultivation, among them stand out traditional cultivation and organic cultivation. The more important factors affecting the strawberries are climate, pests and diseases. Know the types of cultivation and strawberry it is important for decision making in the future, such as use of lichens in cultivation. The aim of this work is to explain about conventional and organic agriculture with emphasis in lichens on strawberry crop to support scientific research in more depth character.> S

  8. Technological process for production of persimmon and strawberry vinegars

    Directory of Open Access Journals (Sweden)

    Claudio Hidalgo

    2010-08-01

    Full Text Available Claudio Hidalgo1, Estibaliz Mateo1, Ana Belen Cerezo2, Maria-Jesús Torija1, Albert Mas11Biotecnologia Enològica, Departament de Bioquimica i Biotecnologia, Facultat d’Enologia, Universitat Rovira i Virgili, Marcel-li Domingo, Tarragona; 2Área de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, SpainAbstract: Fruit surplus is common in intensive agriculture in many countries. This ecologic and economic problem requires alternative uses to be found for fruit. The aim of this study was to use surplus fruit to produce vinegar by traditional methods (alcoholic fermentation and acetification from persimmon and strawberry. The process was performed with naturally occurring microorganisms and compared with inoculated commercial wine yeast for alcoholic fermentation. Alcoholic fermentation proceeded faster when inoculated due to the length of the lag phases observed in spontaneous fermentations. The alcoholic fermentations of strawberry mash were faster than those of persimmon mash. In contrast, acetifications were much faster in persimmon (30 days than in strawberry (70 days, in the latter some acetifications were not finished. From the technologic point of view, to produce persimmon and strawberry wine and vinegar, it is better to avoid fruit pressing and perform the process with fruit mash. Inoculation is recommended for persimmon and is necessary for strawberry.Keywords: wine, vinegar, fruit seasonings, acetic acid bacteria

  9. Development of passion fruit juice beverage

    Science.gov (United States)

    Zhu, Xiang-hao; Duan, Zhen-hua; Yang, Yu-xia; Huang, Xin-hui; Xu, Cheng-ling; Huang, Zhi-zhuo

    2017-12-01

    In this experiment, the whole fruit of passion fruit was used as raw material. The effects of the ratio of material to liquid (RML), the amount of sucrose addition and the pH on the quality of passion fruit juice beverage were investigated by single factor test. And the optimum process conditions of passion fruit juice beverage were determined by orthogonal test. The results show that the optimum process paramenters were as follow: RML was 1:3, pH was 4.0 and sucrose addition was 8%. Under such optimal conditions, the color of passion fruit juice beverage was red, the flavor of passion fruit was rich and it tasted pleasant.

  10. Model-assisted analysis of spatial and temporal variations in fruit temperature and transpiration highlighting the role of fruit development.

    Science.gov (United States)

    Nordey, Thibault; Léchaudel, Mathieu; Saudreau, Marc; Joas, Jacques; Génard, Michel

    2014-01-01

    Fruit physiology is strongly affected by both fruit temperature and water losses through transpiration. Fruit temperature and its transpiration vary with environmental factors and fruit characteristics. In line with previous studies, measurements of physical and thermal fruit properties were found to significantly vary between fruit tissues and maturity stages. To study the impact of these variations on fruit temperature and transpiration, a modelling approach was used. A physical model was developed to predict the spatial and temporal variations of fruit temperature and transpiration according to the spatial and temporal variations of environmental factors and thermal and physical fruit properties. Model predictions compared well to temperature measurements on mango fruits, making it possible to accurately simulate the daily temperature variations of the sunny and shaded sides of fruits. Model simulations indicated that fruit development induced an increase in both the temperature gradient within the fruit and fruit water losses, mainly due to fruit expansion. However, the evolution of fruit characteristics has only a very slight impact on the average temperature and the transpiration per surface unit. The importance of temperature and transpiration gradients highlighted in this study made it necessary to take spatial and temporal variations of environmental factors and fruit characteristics into account to model fruit physiology.

  11. Model-assisted analysis of spatial and temporal variations in fruit temperature and transpiration highlighting the role of fruit development.

    Directory of Open Access Journals (Sweden)

    Thibault Nordey

    Full Text Available Fruit physiology is strongly affected by both fruit temperature and water losses through transpiration. Fruit temperature and its transpiration vary with environmental factors and fruit characteristics. In line with previous studies, measurements of physical and thermal fruit properties were found to significantly vary between fruit tissues and maturity stages. To study the impact of these variations on fruit temperature and transpiration, a modelling approach was used. A physical model was developed to predict the spatial and temporal variations of fruit temperature and transpiration according to the spatial and temporal variations of environmental factors and thermal and physical fruit properties. Model predictions compared well to temperature measurements on mango fruits, making it possible to accurately simulate the daily temperature variations of the sunny and shaded sides of fruits. Model simulations indicated that fruit development induced an increase in both the temperature gradient within the fruit and fruit water losses, mainly due to fruit expansion. However, the evolution of fruit characteristics has only a very slight impact on the average temperature and the transpiration per surface unit. The importance of temperature and transpiration gradients highlighted in this study made it necessary to take spatial and temporal variations of environmental factors and fruit characteristics into account to model fruit physiology.

  12. Gamma irradiation of fruits

    International Nuclear Information System (INIS)

    Beyers, M.

    1983-08-01

    At a Joint FAO/IAEA/WHO Expert Committee on Food Irradiation (JECFI) meeting held in 1976, recommendations were made to rationalize the unnecessarily elaborate wholesomeness evaluation procedures for irradiated foodstuffs. Irradiation at the commercially recommended doses did not adversely affect the constituents of mangoes, papayas, litchis and strawberries at the edible-ripe stage. These favourable radiation-chemical results justified the development of a theoretical model mango which could be used for extrapolation of wholesomeness data from an individual fruit species to all others within the same diet class. Several mathematical models of varying orders of sophistication were evolved. In all of them, it was assumed that the radiant energy entering the system reacted solely with water. The extent of the reaction of the other components of the model fruit with the primary water radicals was then determined. No matter which mathematical treatment was employed, it was concluded that the only components which would undergo significant modification would be the sugars. In order to extrapolate these data from the mango to other fruits, mathematical models of three fruits containing less sugar than the mango, viz. the strawberry, tomato and lemon, were compiled. With these models, the conclusion was reached that the theoretical degradation spectra of these fruits were qualitatively similar to the degradation pattern of the model mango. Theory was again substantiated by the practical demonstration of the protective effect of the sugars in the tomato and lemon. The decrease in radiation damage was enhanced by the mutual protection of the components of the whole synthetic fruits with ultimate protection being afforded by the biological systems of the real fruits

  13. Produção e qualidade do morangueiro em sistemas fechados de cultivo sem solo com emprego de substratos Fruit production and quality of strawberry plants grown in closed soilless growing systems with substrates

    Directory of Open Access Journals (Sweden)

    Rodrigo dos Santos Godoi

    2009-07-01

    Full Text Available O objetivo do trabalho foi determinar o crescimento, a produção e a qualidade das frutas do morangueiro cultivado em três sistemas fechados sem solo e com dois substratos. Os sistemas foram testados no interior de um abrigo telado, no Departamento de Fitotecnia da UFSM, no período entre 27 de abril e 21 de novembro de 2006. Esses sistemas foram constituídos por sacolas fertirrigadas por tubos gotejadores e calhas e leito de cultivo fertirrigados por subirrigação. Foram empregados substrato orgânico Plantmax PXT® e areia. O delineamento experimental utilizado foi um fatorial 3 x 2, com quatro repetições. A fertirrigação foi feita com solução nutritiva completa, sem nenhum descarte durante todo o período experimental, e as frutas foram colhidas maduras. Foram determinados os seguintes parâmetros: a produção, a firmeza, a acidez e o teor de sólidos solúveis totais. Interações significativas entre os sistemas e os substratos foram observadas. Na areia, destacou-se o cultivo nas calhas, com produção de 1017,4g planta-1, sendo 8,13% e 8,33% superior às sacolas e ao leito de cultivo, respectivamente. A produção mais elevada foi de 1196,5g planta-1, obtida com substrato orgânico no leito de cultivo, superior às sacolas em 10,9% e às calhas em 29,33%. Concluiu-se que o cultivo sem solo do morangueiro sem descartes de solução nutritiva é possível e que a produção é influenciada pelo sistema de cultivo e pelo substrato, sem efeitos sobre a qualidade das frutas.The objective of the research was to determine fruit production and quality of strawberry plants grown in three different closed soilless systems and two substrates. The experiment was conducted in a screenhouse at Department of Fitotecnia, UFSM, from April to November, 2006. The soilless growing systems were plastic bags, plastic troughs and growing beds, and the substrates were sand and the organic substrate Plantmax PXT®. A 3 x 2 factorial experimental design

  14. PRODUCCIÓN Y CALIDAD DE FRUTA EN CULTIVARES DE FRESA (Fragaria sp. AFECTADOS POR ESTRÉS SALINO YIELD AND FRUIT QUALITY OF SALT-STRESSED STRAWBERRY CULTIVARS (Fragaria sp.

    Directory of Open Access Journals (Sweden)

    Fánor Casierra-Posada

    2006-12-01

    levels. This trial was carried out to investigate the relationship between NaCl-salt stress, fruit quality and yield on the strawberries cultivars ‘Sweet Carlie’ ‘Chandler’ and ‘Camarosa’ grown in pots filled with salinized soil in glasshouse. Five salt levels were compared (0, 20, 40, 60 and 80 mM NaCl which induced different electrical conductivity (EC levels (2,68; 6,08; 8,24; 9,98 y 12,62 dS.m-1 respectly. The different EC levels were obtained by adding NaCl progressively to the soil. Fruit yield and quality were negatively influenced by the tested treatments. Sugar content in fruits was influenced by the treatments. Total soluble solids and tritrable acidity in fruits grew as EC levels grew. While the accumulation of dry matter in the roots showed a proportional increase with the level of NaCl in the substrate, the fruits showed an opposite tendency. Although typical stress symptoms such as growth retardation and poor fruit yield were observed in the strawberry tested cultivars, ‘Sweet Charlie’ was less sensitive to the salinity.

  15. Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.

    Science.gov (United States)

    Schwieterman, Michael L; Colquhoun, Thomas A; Jaworski, Elizabeth A; Bartoshuk, Linda M; Gilbert, Jessica L; Tieman, Denise M; Odabasi, Asli Z; Moskowitz, Howard R; Folta, Kevin M; Klee, Harry J; Sims, Charles A; Whitaker, Vance M; Clark, David G

    2014-01-01

    Fresh strawberries (Fragaria x ananassa) are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor. In this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating. Analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach. Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively. Overall liking is most greatly influenced by sweetness and strawberry flavor intensity, which are undermined by environmental pressures that reduce sucrose and total volatile content. The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined. Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity. In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively. Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content. These findings allow for consumer influence in the breeding of more desirable fruits and vegetables. Also, this approach garners insights into fruit metabolomics, flavor chemistry, and a paradigm for enhancing liking of natural or processed products.

  16. Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception.

    Directory of Open Access Journals (Sweden)

    Michael L Schwieterman

    Full Text Available Fresh strawberries (Fragaria x ananassa are valued for their characteristic red color, juicy texture, distinct aroma, and sweet fruity flavor. In this study, genetic and environmentally induced variation is exploited to capture biochemically diverse strawberry fruit for metabolite profiling and consumer rating. Analyses identify fruit attributes influencing hedonics and sensory perception of strawberry fruit using a psychophysics approach. Sweetness intensity, flavor intensity, and texture liking are dependent on sugar concentrations, specific volatile compounds, and fruit firmness, respectively. Overall liking is most greatly influenced by sweetness and strawberry flavor intensity, which are undermined by environmental pressures that reduce sucrose and total volatile content. The volatile profiles among commercial strawberry varieties are complex and distinct, but a list of perceptually impactful compounds from the larger mixture is better defined. Particular esters, terpenes, and furans have the most significant fits to strawberry flavor intensity. In total, thirty-one volatile compounds are found to be significantly correlated to strawberry flavor intensity, only one of them negatively. Further analysis identifies individual volatile compounds that have an enhancing effect on perceived sweetness intensity of fruit independent of sugar content. These findings allow for consumer influence in the breeding of more desirable fruits and vegetables. Also, this approach garners insights into fruit metabolomics, flavor chemistry, and a paradigm for enhancing liking of natural or processed products.

  17. Parthenocarpic fruit development in Capsicum annuum

    NARCIS (Netherlands)

    Tiwari, A.

    2011-01-01

    Key words: Parthenocarpy, Capsicum, fruit set, hormones, cell division, cell expansion,

    auxin, gibberellin, temperature, carpel-like structures, genotype

    Parthenocarpy (fruit set without fertilization) is a much desired trait in sweet pepper

    (Capsicum

  18. Phototropin 2 is involved in blue light-induced anthocyanin accumulation in Fragaria x ananassa fruits.

    Science.gov (United States)

    Kadomura-Ishikawa, Yasuko; Miyawaki, Katsuyuki; Noji, Sumihare; Takahashi, Akira

    2013-11-01

    Anthocyanins are widespread, essential secondary metabolites in higher plants during color development in certain flowers and fruits. In strawberries, anthocyanins are also key contributors to fruit antioxidant capacity and nutritional value. However, the effects of different light qualities on anthocyanin accumulation in strawberry (Fragaria x ananassa, cv. Sachinoka) fruits remain elusive. In the present study, we showed the most efficient increase in anthocyanin content occurred by blue light irradiation. Light sensing at the molecular level was investigated by isolation of two phototropin (FaPHOT1 and FaPHOT2), two cryptochrome (FaCRY1 and FaCRY2), and two phytochrome (FaPHYA and FaPHYB) homologs. Expression analysis revealed only FaPHOT2 transcripts markedly increased depending on fruit developmental stage, and a corresponding increase in anthocyanin content was detected. FaPHOT2 knockdown resulted in decreased anthocyanin content; however, overexpression increased anthocyanin content. These findings suggested blue light induced anthocyanin accumulation, and FaPHOT2 may play a role in sensing blue light, and mediating anthocyanin biosynthesis in strawberry fruits. This is the first report to find a relationship between visible light sensing, and color development in strawberry fruits.

  19. Effect of high-oxygen and high-carbon-dioxide atmospheres on strawberry flavor and other quality traits.

    Science.gov (United States)

    Pérez, A G; Sanz, C

    2001-05-01

    The effect of high-oxygen atmospheres on strawberry flavor was studied. Strawberry fruits (Fragariax ananassa Duch. cv. Camarosa) were stored at 8 degrees C in four different atmospheres: air, 5% O(2)/20% CO(2), 80% O(2)/20% CO(2), and 90% O(2)/10% CO(2). Changes in several quality parameters were evaluated. Atmospheres combining high O(2) and high CO(2) were the most effective in preventing fungal growth and enhancing strawberry firmness. Other quality parameters such as color, titrable acidity, sugars and organic acids distribution, off-flavor development, and aroma were only mildly affected by superatmospheric O(2) levels. After one week of storage, unexpected high contents of off-flavor related compounds were found in the 80% O(2)/20% CO(2) and 90% O(2)/10% CO(2) atmospheres. Evidence of an altered ester biosynthesis was also found in fruits stored under these high-O(2) atmospheres. Data obtained suggest that stress induced by high CO(2) and stress induced by high O(2) have an additive effect on strawberry flavor alteration.

  20. Development and Validation of a GC-MS Method for the Analysis of Homogentisic Acid in Strawberry Tree (Arbutus unedo L.) Honey.

    Science.gov (United States)

    Brčić Karačonji, Irena; Jurica, Karlo

    2017-07-01

    To confirm the botanical origin of strawberry tree (Arbutus unedo L.) honey, a liquid-liquid extraction followed by GC-MS method was developed for the quantitative determination of homogentisic acid (HGA), the main phenolic compound in this honey. Different parameters affecting extraction, such as the type and volume of extraction solvents, pH of the solution, and amount of salt, were optimized. The method showed good linearity (r2 = 0.9990) over the tested concentration range (50-500 mg/kg) and a low LOD (0.3 mg/kg). Precision expressed as RSD was <7%. The average accuracy was 95%. The optimized method was applied for determining the HGA content in strawberry tree honey samples from Croatia. The HGA content in analyzed samples (n = 7) ranged from 245.1 to 485.9 mg/kg. The proposed method provided reliable performance and can be easily implemented for the routine monitoring of HGA in strawberry tree honey in order to assure honey QC.

  1. Fruit, seed and embryo development of different cassava (Manihot ...

    African Journals Online (AJOL)

    Fruit, seed and embryo developments of different cassava (Manihot esculenta Crantz) genotypes, as well as embryo rescue, were investigated. The fruits of three genotypes after uncontrolled open pollination presented the same progressive development with similar sizes at different stages. There are large differences in ...

  2. Quality consumption of strawberries (Fragaria ananassa

    Directory of Open Access Journals (Sweden)

    Sergio Carballo

    2011-04-01

    Full Text Available Consumers increasingly require a food product that preserves their nutritional value; retain fresh colour, flavour and texture.Appearance, texture and flavour are attributes identified as to be key to acceptability of fruit and also for consumers in making purchase of fresh produce.The objective of this work was to investigate the consumer's preference of sensory quality and the commercial aspects of fresh strawberries, harvested in spring time from the south of our country, Uruguay- South America.In this study, seven different strawberries varieties (INIA-Arazá, INIA- Ivapitá, Ventana, Seascape, Camarosa, INIA- Ivahé, Aromas were sensory and physicochemical evaluated.The results obtained showed a high correlation between total soluble solids and sensory attributes (colour, texture, and flavour.Camarosa and Ivahé varieties were prefered for the external and internal aspects of the fruit.Future work should investigate other producer areas of the country and other harvesting time.

  3. Identification of food preserved by ionizing radiation; Preliminary tests in strawberries using thermoluminescent (TL)

    International Nuclear Information System (INIS)

    Rubio, T.; Sillano, O.; Espinoza, J.; Roman, A.; Deza, A.

    1992-01-01

    TL measurements, of irradiated and unirradiated strawberries were carried out, using peel, seeds (achenes), leaves and sediment. The four types of samples presented a different thermoluminescence response. The best results were obtained with sediment removed from the soil of the fruit surface. It is concluded that TL is a promising technique for detecting irradiated strawberries using sediment samples. (author)

  4. Characteristics of growing media mixes and application for open-field production of strawberry (Fragaria ananassa)

    Science.gov (United States)

    Pathogen-free growing media are widely used for strawberry production in protected structures in Europe but not common in the United States. There is a need to investigate the feasibility of producing strawberry fruits in open fields with the pathogen-free media in the U.S. The objective of the stud...

  5. Irradiation and evolution of the gray rot botrytis cinerea at the strawberry plant

    International Nuclear Information System (INIS)

    Labidi, Arbia

    2005-01-01

    Strawberry was introduced since french colonization in tunisia as one of plants cultivated. At the end of the 1970's the strawberries (Fragaria ananassa) was developed in area of Cap Bon. grey mold, caused by botrytis cinerea is by far the most widespread and serious of strawberry fruit diseases and an ever-present threat to the crop. A wide variety of symptoms is caused by B. Cinerea such as a rot on fruit and blight on leaves. this fungus causes domages bith in the field and during storage. In order to reduce severity of grey mold, biological control in field and radiation on post-harvest are developed. The objective of this study was to determine the antagonism of some microorganisms against B.Cinerea such as Trichoderma and Bacillus in greenhouse. On the other hand we tested the efficacity of biological products such as Prev-Am and BM 86on enhancing plant defense. For the post-harvest studies, the goal is to provide a wear tool to manage better the fungus by gamma rays radiation. (author). 29 refs

  6. Inspection of fecal contamination on strawberries using fluorescence imaging

    Science.gov (United States)

    Chuang, Yung-Kun; Yang, Chun-Chieh; Kim, Moon S.; Delwiche, Stephen R.; Lo, Y. Martin; Chen, Suming; Chan, Diane E.

    2013-05-01

    Fecal contamination of produce is a food safety issue associated with pathogens such as Escherichia coli that can easily pollute agricultural products via animal and human fecal matters. Outbreaks of foodborne illnesses associated with consuming raw fruits and vegetables have occurred more frequently in recent years in the United States. Among fruits, strawberry is one high-potential vector of fecal contamination and foodborne illnesses since the fruit is often consumed raw and with minimal processing. In the present study, line-scan LED-induced fluorescence imaging techniques were applied for inspection of fecal material on strawberries, and the spectral characteristics and specific wavebands of strawberries were determined by detection algorithms. The results would improve the safety and quality of produce consumed by the public.

  7. Transcriptional control of fleshy fruit development and ripening

    NARCIS (Netherlands)

    Karlova, R.B.; Chapman, N.; David, K.; Angenent, G.C.; Seymour, G.B.; Maagd, de R.A.

    2014-01-01

    Fleshy fruits have evolved to be attractive to frugivores in order to enhance seed dispersal, and have become an indispensable part of the human diet. Here we review the recent advances in the understanding of transcriptional regulation of fleshy fruit development and ripening with a focus on

  8. Postharvest quality of strawberry fruits produced in organic and conventional systems Qualidade pós-colheita de frutos de morangueiro produzidos em sistemas orgânico e convencional

    Directory of Open Access Journals (Sweden)

    Letícia KP Camargo

    2011-12-01

    Full Text Available The aim of this study was to determine some physicochemical characteristics of fruits of eight strawberry cultivars (Aromas, Camino Real, Campinas, Dover, Oso Grande, Toyonoka, Tudla-Milsei and Ventana cultivated in organic and conventional systems. The evaluated characteristics were soluble solids (SS, titratable acidity (TA, SS/AT ratio and anthocyanins. Evaluated sensory characteristics were flavor and appearance. The cultivars had a differential performance between themselves and among the systems for the evaluated characteristics. Generally, the conventional system produced fruit with higher levels of soluble solids and, organic system produced higher concentrations of anthocyanins. The fruits of the cultivar Toyonoka had the best soluble solids content in both systems and the anthocyanins content of 'Tudla-Milsei' and 'Camino Real' were proeminent in comparison to the other cultivars. The fruits from the organic system of production were preferred by judges on the sensorial attributes. The highest notes were given to the appearance of the cultivar Ventana in the conventional system and 'Tudla-Milsei' in the organic system.O presente trabalho teve como objetivo determinar as características químicas e sensoriais de frutos de oito cultivares de morangueiro (Aromas, Camino Real, Campinas, Dover, Oso Grande, Toyonoka, Tudla-Milsei e Ventana provenientes de sistemas orgânico e convencional de cultivo. As características químicas avaliadas foram sólidos solúveis (SS, acidez titulável (AT, relação SS/AT e teor de antocianinas. As características sensoriais avaliadas foram sabor e aparência. As cultivares apresentaram um comportamento diferenciado entre si e frente aos sistemas de produção para as características avaliadas. De modo geral, o sistema convencional produziu frutos com maiores teores de sólidos solúveis e o sistema orgânico, maiores valores de acidez titulável e maiores concentrações de antocianinas. Os frutos da

  9. Desempenho produtivo e massa média de frutos de morangueiro obtidos de diferentes sistemas de cultivo / Performance and average mass of strawberry fruit obtained from different cropping systems

    Directory of Open Access Journals (Sweden)

    Letícia Kurchaidt Pinheiro Camargo

    2010-11-01

    Full Text Available ResumoO trabalho teve como objetivo avaliar a produtividade e a massa média de frutos de oito cultivares de morangueiro (Aromas, Camino Real, Campinas, Dover, Oso Grande, Toyonoka, Tudla-Milsei e Ventana, cultivadas em diferentes sistemas de produção. O delineamento estatístico utilizado foram blocos casualizados com quatro repetições. Os frutos foram colhidos no período de outubro de 2007 a fevereiro de 2008. Os resultados obtidos permitem inferir que, quanto à produtividade, o sistema orgânico foi mais efetivo para as cultivares Oso Grande e Tudla-Milsei e o sistema convencional para Dover e Toyonoka. As maiores massas médias foram encontradas nos frutos das cultivares Tudla-Milsei e Ventana, em ambos os sistemas de cultivo. A cultivar que se destacou tanto no sistema orgânico quanto no sistema convencional, foi a Tudla-Milsei, com as maiores produtividades e os frutos com maior massa média. As cultivares responderam diferentemente em função do manejo cultural empregado em cada sistema de cultivo, o que permite afirmar que há variabilidade entre as cultivares comerciais mais plantadas na atualidade. Portanto, a escolha da cultivar a ser utilizada, visando à produtividade, deverá ocorrer em função do seu desempenho dentro de cada sistema de cultivo. AbstractThe goal of this work was to evaluate the productivity and mass of fruit average of eight cultivars (Aromas, Camino Real, Campinas, Dover, Oso Grande, Toyonoka, Tudla-Milsei and Ventana of strawberry (Fragaria x ananassa grown in different cropping systems. The experimental designed was randomized blocks with 4 replications. The fruits were collected in the period from October 2007 to February 2008. The results allow inferring that the productivity, the organic system was more effective for the Oso Grande e Tudla-Milsei and conventional system for Dover and Toyonoka. The highest masses averages were found in fruits of Tudla-Milsei and Ventana, in both systems. Tudla

  10. Storage of strawberry pollen

    Directory of Open Access Journals (Sweden)

    Rafet Aslantaş

    2013-12-01

    Full Text Available This study was carried out to determine storage ability of strawberry pollen at different temperatures for three different strawberry cultivars 'Aliso', 'Brio', and 'Cruz'. Strawberry pollen was stored at room temperature (22 ±2°C, +4°C, -4°C and -18°C in stabile humidity conditions. Strawberry poIlen was germinated using the hanging drop method in a 20% sucrose solution. Pollen germination rate increased because of low temperature storage. Pollen stored at room temperature and +4°C, -4°C, and -18°C was kept for 8 months, about one year, and 20 months, respectively. Pollen germination rates decreased as the length of storage period increased. The reaction of all cultivars tested on the duration and temperature of storage was similar.

  11. Relationship between production, nematodes and "redness" in strawberries

    Directory of Open Access Journals (Sweden)

    Paula Nogueira Curi

    2016-08-01

    Full Text Available ABSTRACT: In recent years "redness" has increasingly appeared in strawberry plants with leaves taking on a reddish color. No causal agent has been associated with plants. Since strawberries presented problems due to the incidence of nematodes, the purpose of this study was to look at the relationship between production, resistance to the Meloidogyne hapla nematode and the "redness" symptom in strawberry cultivars. Two experiments were performed, both with the 'Camino Real', 'Festival', 'Oso Grande', 'Albion' and 'Camarosa' cultivars. The first experiment was performed in the field, where the following were evaluated: strawberry production, fruit quality, macro and micronutrient contents in fruit and leaves, percentage of plant survival, incidence of nematodes, quantity of eggs in the roots and juveniles in the soil, and the incidence of Botrytis cinerea . In the second experiment, the strawberries were transplanted into pots and filled with pinus bark-based commercial substrate. Half the pots were inocculated with Meloidogyne hapla . Cultivars presented differences in fruit production and also in the incidence of "redness". Lowest performance in production was related to the high incidence of the nematode Meloidogyne hapla. 'Oso Grande' and 'Albion' presented nematode-resistant behavior. It was possible find a relationship between the incidence of the Meloidogyne hapla nematode, and the incidence of "redness" only 'Camino Real' cultivar.

  12. Effect of fungicides on epiphytic yeasts associated with strawberry

    Science.gov (United States)

    Debode, Jane; Van Hemelrijck, Wendy; Creemers, Piet; Maes, Martine

    2013-01-01

    We studied the effect of two commonly used fungicides on the epiphytic yeast community of strawberry. Greenhouse and field experiments were conducted applying Switch (cyprodinil plus fludioxonil) or Signum (boscalid plus pyraclostrobin) to strawberry plants. Yeasts on leaves and fruits were assessed on treated and untreated plants at several time points via plating and denaturing gradient gel electrophoresis (DGGE) analysis. The yeast counts on plates of the treated plants were similar to the control plants. Unripe fruits had 10 times larger yeast concentrations than ripe fruits or leaves. Some dominant yeast types were isolated and in vitro tests showed that they were at least 10 times less sensitive to Switch and Signum as compared with two important fungal strawberry pathogens Botrytis cinerea and Colletotrichum acutatum, which are the targets for the fungicide control. DGGE analysis showed that the applied fungicides had no effect on the composition of the yeast communities, while the growing system, strawberry tissue, and sampling time did affect the yeast communities. The yeast species most commonly identified were Cryptococcus, Rhodotorula, and Sporobolomyces. These results point toward the potential applicability of natural occurring yeast antagonists into an integrated disease control strategy for strawberry diseases.

  13. Plastid Proteomic Analysis in Tomato Fruit Development.

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    Miho Suzuki

    Full Text Available To better understand the mechanism of plastid differentiation from chloroplast to chromoplast, we examined proteome and plastid changes over four distinct developmental stages of 'Micro-Tom' fruit. Additionally, to discover more about the relationship between fruit color and plastid differentiation, we also analyzed and compared 'Micro-Tom' results with those from two other varieties, 'Black' and 'White Beauty'. We confirmed that proteins related to photosynthesis remain through the orange maturity stage of 'Micro-Tom', and also learned that thylakoids no longer exist at this stage. These results suggest that at a minimum there are changes in plastid morphology occurring before all related proteins change. We also compared 'Micro-Tom' fruits with 'Black' and 'White Beauty' using two-dimensional gel electrophoresis. We found a decrease of CHRC (plastid-lipid-associated protein and HrBP1 (harpin binding protein-1 in the 'Black' and 'White Beauty' varieties. CHRC is involved in carotenoid accumulation and stabilization. HrBP1 in Arabidopsis has a sequence similar to proteins in the PAP/fibrillin family. These proteins have characteristics and functions similar to lipocalin, an example of which is the transport of hydrophobic molecules. We detected spots of TIL (temperature-induced lipocalin in 2D-PAGE results, however the number of spots and their isoelectric points differed between 'Micro-Tom' and 'Black'/'White Beauty'. Lipocalin has various functions including those related to environmental stress response, apoptosis induction, membrane formation and fixation, regulation of immune response, cell growth, and metabolism adjustment. Lipocalin related proteins such as TIL and HrBP1 could be related to the accumulation of carotenoids, fruit color and the differentiation of chromoplast.

  14. Investigation of the effect of genotype and agronomic conditions on metabolomic profiles of selected strawberry cultivars with different sensitivity to environmental stress.

    Science.gov (United States)

    Akhatou, Ikram; González-Domínguez, Raúl; Fernández-Recamales, Ángeles

    2016-04-01

    Strawberry is one of the most economically important and widely cultivated fruit crops across the world, so that there is a growing need to develop new analytical methodologies for the authentication of variety and origin, as well as the assessment of agricultural and processing practices. In this work, an untargeted metabolomic strategy based on gas chromatography mass spectrometry (GC-MS) combined with multivariate statistical techniques was used for the first time to characterize the primary metabolome of different strawberry cultivars and to study metabolite alterations in response to multiple agronomic conditions. For this purpose, we investigated three varieties of strawberries with different sensitivity to environmental stress (Camarosa, Festival and Palomar), cultivated in soilless systems using various electrical conductivities, types of coverage and substrates. Metabolomic analysis revealed significant alterations in primary metabolites between the three strawberry cultivars grown under different crop conditions, including sugars (fructose, glucose), organic acids (malic acid, citric acid) and amino acids (alanine, threonine, aspartic acid), among others. Therefore, it could be concluded that GC-MS based metabolomics is a suitable tool to differentiate strawberry cultivars and characterize metabolomic changes associated with environmental and agronomic conditions. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  15. Fate and activity of fungal BCAs delivered to strawberry flowers and their potential for integration with fungicides

    DEFF Research Database (Denmark)

    Jensen, Birgit; Andersen, Birgitte; Thrane, Ulf

    2013-01-01

    Grey mold caused by Botrytis cinerea is a serious strawberry disease. Yield loss is prevented by repeated fungicide treatments during flowering which increases the risk of pesticide residues in berries. Fruit lesions are typically initiated from B. cinerea infected stamens or from dead infected......) the interaction between BCAs and B. cinerea on strawberry flowers, 2) the sensitivity of BCAs to strawberry fungicides, and 3) the effect of combined BCA+fungicide treatment on BCAs and on the indigenous mycobiota....

  16. Strawberry (cv. Romina Methanolic Extract and Anthocyanin-Enriched Fraction Improve Lipid Profile and Antioxidant Status in HepG2 Cells

    Directory of Open Access Journals (Sweden)

    Tamara Y. Forbes-Hernández

    2017-05-01

    Full Text Available Dyslipidemia and oxidation of low density lipoproteins (LDL are recognized as critical factors in the development of atherosclerosis. Healthy dietary patterns, with abundant fruit and vegetable consumption, may prevent the onset of these risk factors due to the presence of phytochemical compounds. Strawberries are known for their high content of polyphenols; among them, flavonoids are the major constituents, and it is presumed that they are responsible for the biological activity of the fruit. Nevertheless, there are only a few studies that actually evaluate the effects of different fractions isolated from strawberries. In order to assess the effects of two different strawberry extracts (whole methanolic extract/anthocyanin-enriched fraction on the lipid profile and antioxidant status in human hepatocellular carcinoma (HepG2 cells, the triglycerides and LDL-cholesterol content, lipid peroxidation, intracellular reactive oxygen species (ROS content and antioxidant enzymes’ activity on cell lysates were determined. Results demonstrated that both strawberry extracts not only improved the lipid metabolism by decreasing triglycerides and LDL-cholesterol contents, but also improved the redox state of HepG2 cells by modulating thiobarbituric acid-reactive substances production, antioxidant enzyme activity and ROS generation. The observed effects were more pronounced for the anthocyanin-enriched fraction.

  17. Nutrient composition of strawberry genotypes cultivated in a horticulture farm.

    Science.gov (United States)

    Hossain, Ashrafi; Begum, Parveen; Salma Zannat, M; Hafizur Rahman, Md; Ahsan, Monira; Islam, Sheikh Nazrul

    2016-05-15

    This article decribes the nutrient composition of four strawberry genotypes cultivated at the Sher-e-Bangla Agriculture University horticulture farm in Dhaka (Bangladesh). AOAC and standard validated methods were employed to analyse the nutrient composition. Protein, fat and ash contents were found to be vary significantly (LSD<0.05), while the variation in moisture (LSD<1.33), dietary fibre (LSD<0.15) and total sugar (LSD<0.09) were found to be insignificant among the genotypes. Vitamin C content ranged from 26.46 mg to 37.77 mg per 100g edible strawberries (LSD<0.060). Amount of carotenoids were found to be very low being in a range of 0.99-3.30 μg per 100g edible fruit. Analysis of mineral revealed that strawberry genotypes contained a wide array of minerals including Ca, Mg, Na, K, P, Mn, Zn, Cu and Fe; most of which varied significantly (LSD<0.05) among the genotypes. Strawberries could be a potential dietary supplement for vitamin C along with minerals, particularly for the children who do not like local fruits, but love to eat the colourful strawberries. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Improved regeneration and transformation protocols for three strawberry cultivars

    Science.gov (United States)

    Zakaria, Hossam; Hussein, Gihan M; Abdel-Hadi, Abdel-Hadi A; Abdallah, Naglaa A

    2014-01-01

    Strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome which makes the breeding of new cultivars difficult. Simple and efficient method for transformation and regeneration is required for cultivars improvement in strawberry. In the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry plants, i.e., Festival, Sweet Charly and Florida via direct organogenesis using the in vitro juvenile leaves as explants. Explants were collected after sub-culturing on a propagation medium composed of MS supplemented with 0.5 mg/l BA; 0.1 mg/l GA3 and 0.1 mg/l IBA. To select the suitable organogenesis, the explants of the three cultivars were cultured on MS medium supplemented with different concentrations of TDZ (1, 2, 3, and 4 mg/l), then incubated at a temperature of 22 °C ± 2. Medium containing 2 mg/l TDZ revealed the best regeneration efficiency with the three cultivars (72% for Festival, and 73% for Sweet Charly and Florida). After 4 weeks, the produced shoots were cultured on MS medium with different concentrations of BA and Kin to enhance shoot elongation. Results showed that the medium containing 1.5 mg/l BA and 0.5 mg/l Kin revealed highest elongation efficiency (88% and 94%) for Festival and Sweet Charly, respectively. On the other hand, medium containing 1.5 mg/l BA and 0.1 mg/l Kin showed highest elongation efficiency (90%) in Florida. Elongated shoots were successfully rooted on MS medium containing 1.5 mg/l NAA. Furthermore, transformation of the two cultivars, Festival and Sweet Charly, has been established via Agrobacterium strain LBA44404 containing the plasmid pISV2678 with gus-intron and bar genes. Three days post co-cultivation, GUS activity was screening using the histochemical assay. The results showed 16% and 18% of the tested plant materials has changed into blue color for Festival and Sweet Charly, respectively. Out of 120 explants only 13 shoots were developed on

  19. Effects of process parameters on supercritical CO2 extraction of total phenols from strawberry (Arbutus unedo L.) fruits: An optimization study.

    Science.gov (United States)

    Akay, Seref; Alpak, Ilknur; Yesil-Celiktas, Ozlem

    2011-08-01

    The aim of this work was to optimize total phenolic yield of Arbutus unedo fruits using supercritical fluid extraction. A Box-Behnken statistical design was used to evaluate the effect of various values of pressure (50-300 bar), temperature (30-80°C) and concentration of ethanol as co-solvent (0-20%) by CO2 flow rate of 15 g/min for 60 min. The most effective variable was co-solvent ratio (p<0.005). Evaluative criteria for both dependent variables (total phenols and radical scavenging activity) in the model were assigned maximum. Optimum extraction conditions were elicited as 60 bar, 48°C and 19.7% yielding 25.72 mg gallic acid equivalent (GAE) total phenols/g extract and 99.9% radical scavenging capacity, which were higher than the values obtained by conventional water (24.89 mg/g; 83.8%) and ethanol (15.12 mg/g; 95.8%) extractions demonstrating challenges as a green separation process with improved product properties for industrial applications. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Influence of processing on the volatile profile of strawberry spreads made with isomaltulose.

    Science.gov (United States)

    Peinado, I; Rosa, E; Heredia, A; Escriche, I; Andrés, A

    2013-05-01

    A new strawberry spread formulated with fructose and isomaltulose (replacing sucrose partially or totally) and a high percentage of fruit was developed in line with the new trend of healthier products. This work studies the influence of some process variables (percentage of sugar, pectin and citric acid, and time of thermal treatment) on the volatile profile of these spreads with different formulations. The ripeness of the raw strawberries influences the concentrations of some of the compounds in the spreads, such as isobutyl acetate, butyl butyrate, 3-hexen-1-yl acetate or propan-2-ol. The process conditions have an important effect on the volatile profiles. Most of the esters and alcohols decreased whereas 13 new compounds appear, mostly furans (furfural, 2-acetylfurane, 5-methyl furfural, mesifurane) and aldehydes (octanal, nonanal, decanal and benzaldeyhde). In general, the spreads formulated with sucrose-isomaltulose that contained higher levels of pectin and citric acid gave better results in the preservation of the original aromatic compounds in raw strawberries. Copyright © 2012 Elsevier Ltd. All rights reserved.

  1. Influence of superabsorbent polymers on the chemical composition of strawberry (Fragaria × ananassa Duch. and biological activity in the soil

    Directory of Open Access Journals (Sweden)

    Mikiciuk Grzegorz

    2015-06-01

    Full Text Available By improving the air and water properties of soils, superabsorbent polymers can affect the increase and improvement of the quality of the yield of berry plants, including strawberries. Their presence in the soil has an influence on its biological activity as related to microorganisms. The aim of the research was to assess the influence of superabsorbent polymers added to the soil on the content of macroelements and sodium in the leaves and fruit of strawberry of the ‘Elsanta’ cultivar and changes in the number of soil bacteria, actinomycetes and fungi. The superabsorbent polymer (AgroHydroGel was used in two doses: 1.8 and 3.6 g dm-3 of soil. The content of phosphorus, potassium, calcium, magnesium and sodium was assessed using the ASA method, while the content of nitrogen and sulphur was assessed by the elemental analysis method (CHNS analyser. The number of microorganisms was assessed with a BacTrac analyser and the coefficient of microorganism development extent (SR was also determined. AgroHydroGel increased the content of nitrogen and potassium in leaves and fruit but did not affect the content of phosphorus, sulphur and sodium. The addition of the superabsorbent at a dose of 3.6 g dm-3 of soil reduced the magnesium content both in the leaves and fruit of the strawberry. AgroHydroGel decreased the content of calcium in the fruit. The use of AgroHydroGel contributed to the expansion of the K ion ratio to other ions, both in the leaves and fruits. We observed a significant increase in the amount of soil bacteria (1.8 g dm-3 dose and no significant influence on actinomycetes and fungi (irrespective of dose used.

  2. Strawberries without struggle

    International Nuclear Information System (INIS)

    Crake, T.

    1980-01-01

    Advice on diseases and their control to make cultivation more profitable and the strawberries more appealing to consumers is given. Control over both leak disease and post-harvest anthracnose have been achieved with a combination of heat treatment and irradiation

  3. Involvement of ethylene biosynthesis and signalling in fruit set and early fruit development in zucchini squash (Cucurbita pepo L.).

    Science.gov (United States)

    Martínez, Cecilia; Manzano, Susana; Megías, Zoraida; Garrido, Dolores; Picó, Belén; Jamilena, Manuel

    2013-09-22

    We have identified a kind of parthenocarpy in zucchini squash which is associated with an incomplete andromonoecy, i.e. a partial conversion of female into bisexual flowers. Given that andromonoecy in this and other cucurbit species is caused by a reduction of ethylene production in the female flower, the associated parthenocarpic development of the fruit suggested the involvement of ethylene in fruit set and early fruit development. We have compared the production of ethylene as well as the expression of 13 ethylene biosynthesis and signalling genes in pollinated and unpollinated ovaries/fruits of two cultivars, one of which is parthenocarpic (Cavili), while the other is non-parthenocarpic (Tosca). In the latter, unpollinated ovaries show an induction of ethylene biosynthesis and ethylene signal transduction pathway genes three days after anthesis, which is concomitant with the initiation of fruit abortion and senescence. Fruit set and early fruit development in pollinated flowers of both cultivars and unpollinated flowers of Cavili is coupled with low ethylene biosynthesis and signalling, which would also explain the partial andromonoecy in the parthenocarpic genotype. The reduction of ethylene production in the ovary cosegregates with parthenocarpy and partial andromonoecy in the selfing progeny of Cavili. Moreover, the induction of ethylene in anthesis (by ethephon treatments) reduced the percentage of bisexual parthenocarpic flowers in Cavili, while the inhibition of ethylene biosynthesis or response (by AVG and STS treatments) induces not only andromonoecy but also the parthenocarpic development of the fruit in both cultivars. Results demonstrate that a reduction of ethylene production or signalling in the zucchini flower is able to induce fruit set and early fruit development, and therefore that ethylene is actively involved in fruit set and early fruit development. Auxin and TIBA treatments, inducing fruit set and early fruit development in this species

  4. Dipping Strawberry Plants in Fungicides before Planting to Control Anthracnose

    Directory of Open Access Journals (Sweden)

    Myeong Hyeon Nam

    2014-03-01

    Full Text Available Anthracnose crown rot (ACR, caused by Colletotrichum fructicola, is a serious disease of strawberry in Korea. The primary inoculums of ACR were symptomless strawberry plants, plant debris, and other host plants. To effectively control anthracnose in symptomless transplanted strawberries, it is necessary to use diseasefree plants, detect the disease early, and apply a fungicide. Therefore, in 2010 and 2011, we evaluated the efficacy of pre-plant fungicide dips by using strawberry transplants infected by C. fructicola for the control of anthracnose. Dipping plants in prochloraz-Mn for 10 min before planting was most effective for controlling anthracnose in symptomless strawberry plants and resulted in more than 76% control efficacy. Azoxystrobin showed a control efficacy of over 40%, but plants treated with pyraclostrobin, mancozeb and iminoctadine tris showed high disease severity. The control efficacy of the dip treatment with prochloraz-Mn did not differ with temperature and time. Treatment with prochloraz-Mn for more than an hour caused growth suppression in strawberry plants. Therefore, the development of anthracnose can be effectively reduced by dipping strawberry plants for 10 min in prochloraz-Mn before planting.

  5. Development of Motorized Oil Palm Fruit Rotary Digester | Asoiro ...

    African Journals Online (AJOL)

    A motorized oil palm fruit rotary digester comprising of a feed hopper, hammers, axle, screening plate, v-belt, 2hp electric motor, digesting chamber and frame was designed and developed using standard and locally sourced materials. The performance test analysis showed that its throughput capacity is 117.93kg/hr with a ...

  6. Strawberry Extract’s Effects on Enterococcus faecalis and Porphyromonas gingivalis Biofilms in vitro

    Directory of Open Access Journals (Sweden)

    Armelia Sari Widyarman

    2017-09-01

    Full Text Available Background: Enterococcus faecalis (E. faecalis and Porphyromonas gingivalis (P. gingivalis are oral bacteria related to root canal infection and periodontal disease pathogenesis. Strawberries (Fragaria x ananassa fruit are rich in vitamins and minerals, have antibacterial and antioxidant effects. Objective: This study investigated the inhibition effect of strawberry extract on monospecies and multispecies E. faecalis and P. gingivalis bacteria grown as biofilms in vitro. Methods: This study used E. faecalis ATCC 29212 and P. gingivalis ATCC 33277. It analyzed the effect of strawberry extract on bacteria biofilm formation using a biofilm assay on microplate wells. Five concentrations of strawberry extracts were used (100%, 50%, 25%, 12.5%, and 6.25%, and the inhibition effect was observed after a 1h, 3h, 6h, and 24h incubation period. Biofilms without the strawberry extract were used as the negative controls, and crystal violet and safranin (0.5%w/v were used to count the biofilm mass. The biofilms grown on microplates were counted using an ELISA reader at 450 nm after 200 mL of 90% ethanol was added to attract the absorbed stain. The strawberry extract inhibition effectiveness on the biofilm formation of each bacterium tested was analyzed using one-way Anova, where p<0.05 was defined as a significant difference. Result: The strawberry extract inhibited the tested monospecies and multispecies bacteria biofilm formation. The optimal strawberry extract concentration for the inhibition of either monospecies biofilms was 100%. However, the optimal incubation time for the strawberry extract to inhibit the multispecies biofilm formation was 24h, which was the study’s biofilm maturity phase. Conclusions: The 100% strawberry extract concentration inhibited the formation of both the monospecies and multispecies E. faecalis and P. gingivalis biofilms. Future studies are needed to evaluate the potential of strawberry extract as an alternative dental

  7. Salicylic Acid Induces Changes in Mango Fruit that Affect Oviposition Behavior and Development of the Oriental Fruit Fly, Bactrocera dorsalis.

    Directory of Open Access Journals (Sweden)

    Kamala Jayanthi Pagadala Damodaram

    Full Text Available The Oriental fruit fly, Bactrocera dorsalis (Hendel is an important quarantine pest around the globe. Although measures for its control are implemented worldwide through IPM and male annihilation, there is little effect on their population. Hence, there is a need for new strategies to control this minacious pest. A strategy that has received negligible attention is the induction of 'natural plant defenses' by phytohormones. In this study, we investigated the effect of salicylic acid (SA treatment of mango fruit (cv. Totapuri on oviposition and larval development of B. dorsalis. In oviposition choice assays, gravid females laid significantly less eggs in SA treated compared to untreated fruit. Headspace volatiles collected from SA treated fruit were less attractive to gravid females compared to volatiles from untreated fruit. GC-MS analysis of the headspace volatiles from SA treated and untreated fruit showed noticeable changes in their chemical compositions. Cis-ocimene and 3-carene (attractants to B. dorsalis were reduced in the headspace volatiles of treated fruit. Further, reduced pupae formation and adult emergence was observed in treated fruit compared to control. Increased phenol and flavonoid content was recorded in treated fruit. We also observed differential expression of anti-oxidative enzymes namely catalase (CAT, polyphenoloxidase (PPO and peroxidase (POD. In summary, the results indicate that SA treatment reduced oviposition, larval development and adult emergence of B. dorsalis and suggest a role of SA in enhancing mango tolerance to B. dorsalis.

  8. Direct and Pollinator-Mediated Effects of Herbivory on Strawberry and the Potential for Improved Resistance

    Directory of Open Access Journals (Sweden)

    Anne Muola

    2017-05-01

    Full Text Available The global decline in pollinators has partly been blamed on pesticides, leading some to propose pesticide-free farming as an option to improve pollination. However, herbivores are likely to be more prevalent in pesticide-free environments, requiring knowledge of their effects on pollinators, and alternative crop protection strategies to mitigate any potential pollination reduction. Strawberry leaf beetles (SLB Galerucella spp. are important strawberry pests in Northern Europe and Russia. Given that SLB attack both leaf and flower tissue, we hypothesized pollinators would discriminate against SLB-damaged strawberry plants (Fragaria vesca, cultivar ‘Rügen’, leading to lower pollination success and yield. In addition we screened the most common commercial cultivar ‘Rügen’ and wild Swedish F. vesca genotypes for SLB resistance to assess the potential for inverse breeding to restore high SLB resistance in cultivated strawberry. Behavioral observations in a controlled experiment revealed that the local pollinator fauna avoided strawberry flowers with SLB-damaged petals. Low pollination, in turn, resulted in smaller more deformed fruits. Furthermore, SLB-damaged flowers produced smaller fruits even when they were hand pollinated, showing herbivore damage also had direct effects on yield, independent of indirect effects on pollination. We found variable resistance in wild woodland strawberry to SLB and more resistant plant genotypes than the cultivar ‘Rügen’ were identified. Efficient integrated pest management strategies should be employed to mitigate both direct and indirect effects of herbivory for cultivated strawberry, including high intrinsic plant resistance.

  9. Identifying haplotypes for flowering and QTLs for fruit quality in the RosBREED Michigan and Oregon strawberry (Fragaria ×ananassa) breeding sets using pedigree-based analysis [abstract

    Science.gov (United States)

    Strawberry (Fragaria ×ananassa) is consumed for its flavor and health benefits. Over the last two decades, several quantitative trait loci (QTL) analysis studies for consumer traits were conducted using low-density genetic maps. The previous studies utilized low-throughput genotyping methodologies. ...

  10. Testing fruit quality by photoacoustic spectroscopy assay

    International Nuclear Information System (INIS)

    Popa, C; Dumitras, D C; Patachia, M; Banita, S

    2014-01-01

    This study was conducted with the aim of testing the hypothesis that raspberry and strawberry fruits from nonorganic farming release more ethylene gas compounds compared to organic ones. At the same time, the experiments focused on evaluation of the potential and capabilities of the laser photoacoustic spectroscopy (LPAS) method in the assessment of fruit quality related to the effects of nitrogen. Ethylene gas can be harmful and carcinogenic, because it can accelerate the natural ripening process of physiologically mature fruits and makes the fruits more consistent in size. With the advantages of LPAS, we demonstrate that the concentration of ethylene from nonorganic raspberry and strawberry fruits is greater than from organic ones. (paper)

  11. Anatomy and fruit development in Schinopsis balansae (Anacardiaceae

    Directory of Open Access Journals (Sweden)

    Vesprini, José Luis

    2010-12-01

    Full Text Available Schinopsis balansae Engl. is a dioecious tree; reproduction is by woody samaras containing a single seed. Fruit set is high, even in isolated trees, empty fruits are frequent and account for a high proportion of the total fruit production. To describe the anatomy and the ontogeny of seeded and seedless fruits, flowers and fruits at different development stages from bagged and pollen exposed flowers were studied. The development and the anatomy of the pericarp in seeded and parthenocarpic fruits did not differ. It consisted in an exocarp formed sensu lato from the external epidermis of the ovary and some layers of the underlying parenchyma. The mature mesocarp was constituted by a sclerified tissue and lysigenous channels. The fruit is of Anacardium type: the endocarp presented three sclerenchymatic and a crystalliferous layer. The endocarp development was also sensu lato because it was formed from the epidermis and the hypodermis of the carpel. This organization agrees with the only species described in the genus: S. haenkeana Engl. Schinopsis balansae is able to produce parthenocarpic fruits in the absence of pollination. Empty fruits from free exposed flowers presented embryos arrested at different stages, although total absence of an embryo was the most common condition. Parthenocarpy seems to be a plesiomorphic trait within the Anacardiaceae, but regardless of its origin, the maintenance of empty fruit production in a wind-dispersed samara is difficult to explain. Whether parthenocarpy has an adaptive value, or is an evolutionary constraint remains unclear in Schinopsis.Schinopsis balansae Engl. es un árbol dioico, cuya reproducción se realiza a través de sámaras uniseminadas. La producción de frutos es muy alta y una gran proporción de los mismos son vanos. Con la finalidad de describir la anatomía y ontogenia de los frutos, con y sin semillas, se trabajó con flores y frutos en distintos estados de desarrollo. Se utilizaron

  12. Effect of Chitosan Coating Treatments and Calcium Dips on Quality and Shelf-life of Strawberries

    International Nuclear Information System (INIS)

    Swailam, H.M.

    2008-01-01

    Strawberries are a highly perishable fruit and storage life may be less than a week. In these investigations strawberries were treated with one of the following treatments: I) 1 % calcium chloride dips (1 % CaCl 2 ), II) 2 % irradiated (150 kGy in the solid state) chitosan (CS) (2 % Irr. CS) and III) with a coating formulation containing 2 % Irr. CS + 1 % CaCl 2 . They were then stored at 6 degree C for up to 24 days. The effectiveness of the different treatments was assessed by evaluating the microbiological, physicochemical and sensory properties of strawberries during the storage period. Results indicated that no sign of strawberries decay were observed in the fruits treated with CS formulation containing 2 % Irr. CS + 1 % CaCl 2 up to 20 days, where 6.1 % wt loss was detected. This treatment slowed the ripening of strawberries as shown by their retention of firmness and delayed changes in their external colour anthocyanin. To a lesser extent titratable acidity and ph were also affected by coatings. This treatment decreased the initial log counts of total aerobic bacteria, mould and yeast, coliform and E. coli. After 20 days of cold storage, the log counts of these micro organisms were lower than the log count of uncoated strawberries. Thus, CS can be used as a natural antimicrobial coating on fresh strawberries to improve microbiological quality and extend shelf-life. CS formulation contains 2 % Irr. CS + 1 % CaCl 2 extended the shelf-life of strawberries to 20 days in cold storage at 6 degree C with acceptable appearance, firmness, flavour and colour, while uncoated strawberries only lasted for 4 days as a result of mould growth and loss of surface appearance

  13. MYB Transcription Factors in Chinese Pear (Pyrus bretschneideri Rehd.: Genome-Wide Identification, Classification and Expression Profiling during Fruit Development

    Directory of Open Access Journals (Sweden)

    Yun Peng eCao

    2016-04-01

    Full Text Available The MYB family is one of the largest families of transcription factors in plants. Although some MYBs have been reported to play roles in secondary metabolism, no comprehensive study of the MYB family in Chinese pear (Pyrus bretschneideri Rehd. has been reported. In the present study, we performed genome-wide analysis of MYB genes in Chinese pear, designated as PbMYBs, including analyses of their phylogenic relationships, structures, chromosomal locations, promoter regions, GO annotations and collinearity. A total of 129 PbMYB genes were identified in the pear genome and were divided into 31 subgroups based on phylogenetic analysis. These PbMYBs were unevenly distributed among 16 chromosomes (total of 17 chromosomes. The occurrence of gene duplication events indicated that whole-genome duplication and segmental duplication likely played key roles in expansion of the PbMYB gene family. Ka/Ks analysis suggested that the duplicated PbMYBs mainly experienced purifying selection with restrictive functional divergence after the duplication events. Interspecies microsynteny analysis revealed maximum orthology between pear and peach, followed by plum and strawberry. Subsequently, the expression patterns of 20 PbMYB genes that may be involved in lignin biosynthesis according to their phylogenetic relationships were examined throughout fruit development. Among the twenty genes examined, PbMYB25 and PbMYB52 exhibited expression patterns consistent with the typical variations in the lignin content previously reported. Moreover, sub-cellular localization analysis revealed that two proteins PbMYB25 and PbMYB52 were localized to the nucleus. All together, PbMYB25 and PbMYB52 were inferred to be candidate genes involved in the regulation of lignin biosynthesis during the development of pear fruit. This study provides useful information for further functional analysis of the MYB gene family in pear.

  14. Impact of High-Power Pulsed Light on Microbial Contamination, Health Promoting Components and Shelf Life of Strawberries

    Directory of Open Access Journals (Sweden)

    Irina Buchovec

    2013-01-01

    Full Text Available The aim of this work is to evaluate the impact of high-power pulsed light (HPPL on the microbial control and nutritional properties of strawberries. Berries were treated with HPPL and afterwards analyzed in terms of microbial contamination, shelf life extension, antioxidant capacity, firmness, total phenolic, total anthocyanin and ascorbic acid content, and colour. Results indicate that the decontamination of strawberries by HPPL was significant compared to control. Naturally distributed mesophilic bacteria on the surface of strawberries were inactivated by 2.2 log, and inoculated Bacillus cereus and Listeria monocytogenes were inactivated by 1.5 and 1.1 log, respectively. Yeasts/microfungi distributed on the surface of strawberries were inactivated by 1 log. The shelf life of treated strawberries was extended by 2 days. The increase of temperature on the surface of fruit never exceeded 42 °C. No significantly important differences were observed in total phenolic, total anthocyanin and ascorbic acid content, and antioxidant capacity of strawberry fruits before and after pulsed light treatment. Moreover, no impact on the strawberry colour or firmness was found after HPPL treatment. In conclusion, HPPL is fast, effective, non-thermal and environmentally friendly technique which can be applied for microbial control of strawberries.

  15. Effect of gamma irradiation on storability of strawberry (Fragaria sp)

    Energy Technology Data Exchange (ETDEWEB)

    Al-Bachir, M; Farah, S [Atomic Energy Commission, Dept. of Agriculture, Damascus (Syrian Arab Republic)

    1998-02-01

    Despite the increased production of strawberry in Syria, the storability and marketability of fruits were not well studied. The objectives of this study were to investigate the effect of gamma irradiation on storability of Senga sengana strawberry produced in Syria and the effect of gamma irradiation on fungal sp. i.e. Botrytis; Penicillium; Rhizopus. The fruits were treated with 1 , 2 and 3 KGy of gamma rays. Treated and untreated fruits were stored at 2 to 4 centigrade and 80 to 90 % relative humidity (RH). In order to investigate their marketability, the fruits where held at room temperature (25 to 30 centigrade). Weight loss, microbial decay, and total loss, juice production, pH, total soluble solids of the juice and organoleptic qualities were evaluated throughout the different storage and marketing periods. The results indicate that gamma irradiation decreased the microbial decay and increased the storability and marketability of fruits by 50 and 100% after using 2 and 3 kGy, respectively. D10 were 1.8 and 2.4 for Botrytis and Rhizopus respectively. One day after irradiation total soluble solids and its pH values were increased. Fourteen days later, irradiated fruits produced more juice with higher pH, but total soluble solids were less. Gamma irradiation did not have an effect on aroma and colour of fruits, whereas, 3 kGy of gamma irradiation had an adverse negative effect on taste. (author)

  16. Thermal requirements and estimate of the annual number of generations of Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) on strawberry crop

    International Nuclear Information System (INIS)

    Nondillo, Aline; Redaelli, Luiza R.; Pinent, Silvia M.J.; Gitz, Rogerio

    2008-01-01

    Frankliniella occidentalis (Pergande) is one of the major strawberry pests in southern Brazil. The insect causes russeting and wither in flowers and fruits reducing commercial value. In this work, the thermal requirements of the eggs, larvae and pupae of F. occidentalis were estimated. Thrips development was studied in folioles of strawberry plants at six constant temperatures (16, 19, 22, 25, 28 and 31 deg C) in controlled conditions (70 +- 10% R.H. and 12:12 L:D). The number of annual generations of F. occidentalis was estimated for six strawberry production regions of Rio Grande do Sul State based on its thermal requirements. Developmental time of each F. occidentalis stages was proportional to the temperature increase. The best development rate was obtained when insects were reared at 25 deg C and 28 deg C. The lower threshold and the thermal requirements for the egg to adult stage were 9.9 deg C and 211.9 degree-days, respectively. Considering the thermal requirements of F. occidentalis, 10.7, 12.6, 13.1, 13.6, 16.5 and 17.9 generations/year were estimated, respectively, for Vacaria, Caxias do Sul, Farroupilha, Pelotas, Porto Alegre and Taquari producing regions located in Rio Grande do Sul State, Brazil. (author)

  17. Post-harvest conservation of organic strawberries coated with cassava starch and chitosan

    Directory of Open Access Journals (Sweden)

    Raquel P Campos

    2011-10-01

    Full Text Available The strawberry is as non-climacteric fruit, but has a high post-harvest respiration rate, which leads to a rapid deterioration at room temperature. This study aimed to evaluate the application of biodegradable coating on postharvest conservation of organic strawberries, cv. Camarosa, packed in plastic hinged boxes and stored at 10ºC. The treatments consisted of: a control; b 2% cassava starch; c 1% chitosan; and d 2% cassava starch + 1% chitosan. Physical and chemical characteristics of fruits were evaluated at 3, 6 and 9 days of storage, and microbiological and sensory analyses were carried out at the end of the storage period. The treatments influenced positively the post-harvest quality of organic strawberries. The coating cassava starch + chitosan provided the best results, with less than 6% of loss in fruit mass, lower counts of yeast and psychrophilic microorganisms and the best appearance according to the sensory analysis.

  18. Study of Trehalose Addition on Aroma Retention in Dehydrated Strawberry Puree

    Directory of Open Access Journals (Sweden)

    Draženka Komes

    2003-01-01

    Full Text Available In order to improve the quality of dehydrated fruit products, the influence of the addition of two sugars (sucrose and trehalose on the retention of aroma components during dehydration of strawberry puree was investigated. Manual headspace solid-phase microextraction (SPME, containing polydimethylsiloxane coated fibre (100 μm coupled with gas chromatography (GC-FID and GC-MS was used for the analysis of the aroma of strawberry puree dehydrated by using freeze drying and foam-mat drying. The analytes identified included esters, carbonyl compounds, terpenoids, several alcohols and acids. The results obtained in this study give further insight into the mechanisms concerning the application of trehalose as flavouring additive, due to its ability to retain and preserve the fruit volatiles responsible for the characteristic flavour of fresh fruits during dehydration processes. The best retention of aroma components in dehydrated strawberry puree was obtained by trehalose addition when combined with freeze drying.

  19. Influence of Fermentation Process on the Anthocyanin Composition of Wine and Vinegar Elaborated from Strawberry.

    Science.gov (United States)

    Hornedo-Ortega, Ruth; Álvarez-Fernández, M Antonia; Cerezo, Ana B; Garcia-Garcia, Isidoro; Troncoso, Ana M; Garcia-Parrilla, M Carmen

    2017-02-01

    Anthocyanins are the major polyphenolic compounds in strawberry fruit responsible for its color. Due to their sensitivity, they are affected by food processing techniques such as fermentation that alters both their chemical composition and organoleptic properties. This work aims to evaluate the impact of different fermentation processes on individual anthocyanins compounds in strawberry wine and vinegar by UHPLC-MS/MS Q Exactive analysis. Nineteen, 18, and 14 anthocyanin compounds were identified in the strawberry initial substrate, strawberry wine, and strawberry vinegar, respectively. Four and 8 anthocyanin compounds were tentatively identified with high accuracy for the 1st time to be present in the beverages obtained by alcoholic fermentation and acetic fermentation of strawberry, respectively. Both, the total and the individual anthocyanin concentrations were decreased by both fermentation processes, affecting the alcoholic fermentation to a lesser extent (19%) than the acetic fermentation (91%). Indeed, several changes in color parameters have been assessed. The color of the wine and the vinegar made from strawberry changed during the fermentation process, varying from red to orange color, this fact is directly correlated with the decrease of anthocyanins compounds. © 2017 Institute of Food Technologists®.

  20. Pathogenic and Nonpathogenic Lifestyles in Colletotrichum acutatum from Strawberry and Other Plants.

    Science.gov (United States)

    Freeman, S; Horowitz, S; Sharon, A

    2001-10-01

    ABSTRACT Anthracnose is one of the major fungal diseases of strawberry occurring worldwide. In Israel, the disease is caused primarily by the species Colletotrichum acutatum. The pathogen causes black spot on fruit, root necrosis, and crown rot resulting in mortality of transplants in the field. The host range and specificity of C. acutatum from strawberry was examined on pepper, eggplant, tomato, bean, and strawberry under greenhouse conditions. The fungus was recovered from all plant species over a 3-month period but caused disease symptoms only on strawberry. Epiphytic and endophytic (colonization) fungal growth in the different plant species was confirmed by reisolation from leaf tissues and by polymerase chain reaction (PCR)-specific primer amplification. C. acutatum was also isolated from healthy looking, asymptomatic plants of the weed genera Vicia and Conyza. Isolates that were recovered from the weeds caused disease symptoms on strawberry and were positively identified as C. acutatum by PCR. The habitation of a large number of plant species, including weeds, by C. acutatum suggests that, although it causes disease only on strawberry and anemone in Israel, this fungus can persist on many other plant species. Therefore, plants that are not considered hosts of C. acutatum may serve as a potential inoculum source for strawberry infection and permit survival of the pathogen between seasons.

  1. A dynamic interplay between phytohormones is required for fruit development, maturation and ripening

    Directory of Open Access Journals (Sweden)

    Peter eMcAtee

    2013-04-01

    Full Text Available Plant species that bear fruit often utilise expansion of an ovary (carpel or accessory tissue as a vehicle for seed dispersal. While the seed(s develop, the tissue(s of the fruit follow a common progression of cell division and cell expansion, promoting growth of the fruit. Once the seed is fully developed, the fruit matures and the surrounding tissue either dries or ripens promoting the dissemination of the seed. As with many developmental processes in plants, plant hormones play an important role in the synchronisation of signals between the developing seed and its surrounding fruit tissue(s, regulating each phase of fruit development. Following pollination, fruit set is achieved through a de-repression of growth and an activation of cell division via the action of auxin and/or cytokinin and/or gibberellin. Following fruit set, growth of the fruit is facilitated through a relatively poorly studied period of cell expansion and endoreduplication that is likely regulated by similar hormones as in fruit set. Once the seeds reach maturity, fruit become ready to undergo ripening and during this period there is a major switch in relative hormone levels of the fruit, involving an overall decrease in auxin, gibberellin and cytokinin and a simultaneous increase in abscisic acid and ethylene. While the role of hormones in fruit set and ripening is well documented, the knowledge of the roles of other hormones during growth, maturation and some individual ripening components is sketchy.

  2. Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca

    Directory of Open Access Journals (Sweden)

    Kui eLin-Wang

    2014-11-01

    Full Text Available The woodland strawberry, Fragaria vesca is a model fruit for a number of rosaceous crops. We have engineered altered concentrations of anthocyanin in F. vesca, to determine the impact on plant growth and fruit quality. Anthocyanin concentrations were significantly increased by over-expression or decreased by knock-down of the R2R3 MYB activator, MYB10. In contrast, a potential bHLH partner for MYB10 (bHLH33 did not affect the anthocyanin pathway when knocked down using RNAi constructs. Metabolic analysis of fruits revealed that, of all the polyphenolics surveyed, only cyanidin and pelargonidin glucoside, and coumaryl hexose were significantly affected by over-expression and knock down of MYB10. Using the F. vesca genome sequence, members of the MYB, bHLH and WD40 families were examined. Global analysis of gene expression and targeted qPCR analysis of biosynthetic genes and regulators confirmed the effects of altering MYB10 expression, as well as the knock-down of bHLH33. Other members of the MYB transcription factor family were affected by the transgenes. Transient expression of strawberry genes in Nicotiana benthamiana revealed that MYB10 can auto-regulate itself, and potential repressors of MYB10. In tobacco, MYB10’s activation of biosynthetic steps is inhibited by the strawberry repressor MYB1.

  3. The Use of Arbuscular Mycorrhizal Fungi to Improve Strawberry Production in Coir Substrate

    Directory of Open Access Journals (Sweden)

    Louisa Robinson Boyer

    2016-08-01

    Full Text Available Strawberry is an important fruit crop within the UK. To reduce the impact of soil-borne diseases and extend the production season, more than half of the UK strawberry production is now in substrate (predominantly coir under protection. Substrates such as coir are usually depleted of microbes including arbuscular mycorrhizal fungi (AMF and consequently the introduction of beneficial microbes is likely to benefit commercial cropping systems. Inoculating strawberry plants in substrate other than coir has been shown to increase plants tolerance to soil-borne pathogens and water stress. We carried out studies to investigate whether AMF could improve strawberry production in coir under low nitrogen input and regulated deficit irrigation. Application of AMF led to an appreciable increase in the size and number of class I fruit, especially under either deficient irrigation or low nitrogen input condition. However, root length colonisation by AMF was reduced in strawberry grown in coir compared to soil and Terragreen. Furthermore, the appearance of AMF colonising strawberry and maize roots grown in coir showed some physical differences from the structure in colonised roots in soil and Terragreen: the colonization structure appeared to be more compact and smaller in coir.

  4. Thermal requirements and estimated number of generations of Neopamera bilobata (Say in strawberry-producing regions of Brazil

    Directory of Open Access Journals (Sweden)

    Taciana Melissa de Azevedo Kuhn

    2017-12-01

    Full Text Available ABSTRACT: The thermal threshold and thermal requirements of Neopamera bilobata were determined, and the number of generations that this species may produce in the main strawberry-producing regions of Brazil was estimated. In a climate chamber (70±10% RH and 12h photophase at 16, 19, 22, 25, 28, or 30±1°C, the development of 120 eggs was monitored until the adult stage, at each temperature. Nymphs were maintained in individual cages and fed on strawberry fruits of the cultivar Aromas. The mean duration and viability of the egg and nymph stages were calculated by estimating the lower and upper developmental thresholds and the thermal constant, and this information was used to estimate the number of generations per year in different strawberry-producing regions of Brazil. The egg-to-adult duration decreased as temperatures increased, up to 28°C (93.4, 83.2, 43.9, and 31.4 days at 19, 22, 25, and 28°C, respectively. Viability of nymphs was highest between 22 and 28°C. At 30°C, the egg-to-adult duration increased (36 days, while the viability decreased (11.11%. The lower egg-to-adult developmental threshold was 15.2°C and the thermal constant was 418.4 degree-days. Calculating the number of generations indicated that the largest number (5.1 generations yr-1 was obtained for the municipality of Jaboti, Paraná, and the smallest for Caxias do Sul, Rio Grande do Sul (1.9 generations yr-1. Our findings demonstrated that important strawberry-producing regions in Brazil are suitable for the development of N. bilobata.

  5. L-Ascorbic acid metabolism during fruit development in an ascorbate-rich fruit crop chestnut rose (Rosa roxburghii Tratt).

    Science.gov (United States)

    Huang, Ming; Xu, Qiang; Deng, Xiu-Xin

    2014-09-01

    Chestnut rose (Rosa roxburghii Tratt) is a fruit crop that contains unusually high levels of l-ascorbic acid (AsA; ∼1300 mg 100g(-1) FW). To explore the mechanisms underlying AsA metabolism, we investigated the distribution and abundance of AsA during fruit development. We also analyzed gene expression patterns, enzyme activities, and content of metabolites related to AsA biosynthesis and recycling. AsA first accumulated during late fruit development and continued to accumulate during ripening, with the highest accumulation rate near fruit maturity. The redox state of AsA in fruit was also enhanced during late fruit development, while leaf and other tissues had much lower levels of AsA and the redox state of AsA was lower. In mature fruit, AsA was mainly distributed in the cytoplasm of the mesocarp. Correlation analysis suggested that the gene expression patterns, enzyme activities, and related metabolite concentrations involved in the l-galactose pathway showed relatively high correlations with the accumulation rate of AsA. The gene expression pattern and activity of dehydroascorbate reductase (DHAR, EC 1.8.5.1) correlated strongly with AsA concentration, possibly indicating the crucial role of DHAR in the accumulation of high levels of AsA in chestnut rose fruit. Over expression of DHAR in Arabidopsis significantly increased the reduced AsA content and redox state. This was more effective than over expression of the l-galactose pathway gene GDP-d-mannose-3,5-epimerase (EC 5.1.3.18). These findings will enhance understanding of the molecular mechanisms regulating accumulation of AsA in chestnut rose. Copyright © 2014 Elsevier GmbH. All rights reserved.

  6. Effects of Lavender Essential Oil and Methyl Salicylate on Gray Mold Control and Postharvest Quality of Strawberry

    Directory of Open Access Journals (Sweden)

    M. Sayyari

    2016-07-01

    Full Text Available Introduction: Strawberry (fragaria×ananassa Duch. fruit characterized by short storage life, often estimated last less than one week even under optimum conditions at 8°C. The loss of fruit quality is often caused by gray mold (Botrytis cinerea that is the most frequent reported postharvest disease in strawberry during storage (6. In recent years, considerable attention has given to elimination of synthetic chemical and fungicides application and development of various alternative strategies for controlling fruit and vegetables diseases (2. One strategy is replacement of natural products with plant origin such as essential oil and methyl salicylate (MeSA. Essential oils are volatile, natural and complex compounds characterized by a strong odor formed by aromatic plants in form of secondary metabolites. In nature, essential similar oils that extract from lavender (Lavandula angustifolia play an important role in protection of the plants against pathogen incidence that can be replaced by synthetic fungicides (1, 4 and 14. MeSA is also a volatile natural compound synthesized from salicylic acid which has an important role in the plant defense-mechanism, as well as plant growth and development (5, 19 and 20. Therefore, the main objective of this research was to study the effects of MeSA and lavender essential oil (LEO on decay control caused by Botrytis cinerea as well as post-harvest quality indices of strawberry fruits during cold storage. Material and Methods: First, antifungal activity was studied by using a contact assay (in vitro, which produces hyphal growth inhibition. Briefly, potato dextrose agar (PDA plates were prepared using 8 cm diameter glass petri dishes and inhibitory percentage was determined. For in-vivo assessment of LEO and MeSA effects on Botrytis-caused fungal disease control, the experiment was conducted as factorial in completely randomized design (CRD with 3 replicates. The treatments were 3 concentration of LEO including

  7. Effect of Pretreatments on Seed Viability During Fruit Development ...

    African Journals Online (AJOL)

    Fiifi Baidoo

    and petri dishes on moist filter paper, gave 80% germination in variety gabonensis, 20 weeks after fruiting. .... Effect of pretreatments on seed germination of fruits of Irvingia gabonensis plucked from forest ...... A review of the tariff structure and.

  8. Preservação da qualidade pós-colheita de araçá-vermelho através do tratamento com 1-metilciclopropeno e do acondicionamento em embalagens plásticas, sob refrigeração Postharvest quality preservation of red strawberry-guavas by treatment with 1-methylcyclopropene and fruit packaging in plastic films under refrigeration

    Directory of Open Access Journals (Sweden)

    Cassandro Vidal Talamini do Amarante

    2009-12-01

    Full Text Available O objetivo deste trabalho foi estudar o padrão respiratório e de produção de etileno, e os efeitos do tratamento com diferentes doses de 1-metilciclopropeno (1-MCP e do acondicionamento em diferentes embalagens plásticas, associado à refrigeração, na preservação da qualidade pós-colheita de araçá-vermelho. Os frutos apresentaram comportamento climatérico de respiração e produção de etileno. Frutos tratados com doses crescentes de 1-MCP (0; 100; 300; 600 e 1.200 nL L-1 e armazenados a 10±1C/90±5% UR, durante 10 dias, apresentaram retardo substancial no amadurecimento, através da preservação da textura e inibição na mudança de cor da epiderme. Frutos acondicionados com diferentes filmes (polietileno de baixa densidade, policloreto de vinila e à base de náilon apresentaram, em termos gerais, melhor preservação da textura e menores mudanças na cor da epiderme e perdas de massa fresca, durante armazenamento refrigerado (5C e 10C. Os resultados demonstram que os frutos de araçá-vermelho apresentam elevada perecibilidade, caracterizada pelas elevadas taxas respiratórias e de produção de etileno, sendo mais bem preservados quando refrigerados, e tratados com 1-MCP ou acondicionados em embalagens plásticas.This work was carried out to study respiration and ethylene production behavior of red strawberry-guavas and to assess the preservation of fruit postharvest quality by treatment with different doses of 1-methylcyclopropene (1-MCP and fruit packaging in plastic films, under refrigeration. Red strawberry-guavas exhibit a climacteric behavior of respiration and ethylene production. Fruits treated with increasing doses of 1-MCP (0, 100, 300, 600, and 1,200 nL L-1 and stored at 10±1C/90±5% RH for 10 days had a substantial delay in ripening, with a better retention of texture and inhibition in the change of the color of the skin. Fruits packed in different plastic films (low density polyethylene, vinyl polychloride, and

  9. Effect of hormone treatments on deformed fruit development in pear

    African Journals Online (AJOL)

    erica

    2012-05-31

    May 31, 2012 ... of plant growth regulators applications and endogenous hormones on deformed pear fruit to clarify the relation- ship between shape and hormones in the fruit. MATERIALS AND METHODS. The experiments were conducted in 2008 using pear trees growing fruit demonstration farm in Jianning County.

  10. Manipulating transplant morphology to advance post-transplant growth and yield in strawberry

    NARCIS (Netherlands)

    Reekie, J.Y.

    2005-01-01

    Two methods were developed to enhance transplant success and minimize water use of strawberry transplants harvested in Canadian nurseries for use in the annual strawberry production system in the Southern United States: mechanical leaf removal by mowing, and chemical control of growth and

  11. Processing factors and variability of pyrimethanil, fenhexamid and tolylfluanid in strawberries

    DEFF Research Database (Denmark)

    Christensen, Hanne Bjerre; Granby, Kit; Rabølle, M.

    2003-01-01

    An HPLC-MS/MS method for the analysis of three pesticides in strawberries was developed and validated. Recoveries were measured at three spiking levels and ranged from 85 to 99% (mean recoveries). The effects of processing of strawberries ranging from rinsing to jam production were investigated...

  12. CATT as a non-chemical pest and nematode control method in strawberry mohter planting stock

    NARCIS (Netherlands)

    Kruistum, van G.; Hoek, J.; Verschoor, J.A.

    2014-01-01

    Until 2008 methyl bromide (MeBr) was used in The Netherlands for fumigation of strawberry runners, intended as mother planting stock, to make them free of contamination by strawberry tarsonemid mites (Phytonemus pallidus). As an alternative of MeBr fumigation a 48 h CATT method was developed and

  13. Chemical evaluation of strawberry plants produced by tissue culturing of gamma irradiated seedlings

    International Nuclear Information System (INIS)

    Maraei, R.W.

    2007-01-01

    studies were conducted to evaluate the influence of gamma irradiation as a supplementary factor precedes tissue culture application on strawberry seedlings (c.v.Rosa Linda). the strawberry seedling were irradiated using 8 doses of co 60 gamma rays 50.75.100.125 ,150,250, 350 and 500 gray. tissue culture technique was applied on irradiated and unirradiated strawberry seedling. different characteristics of plantlets, plant and fruit of strawberry produced from the double treatment (irradiation followed by tissue culture) were studied as well as the early, total and exportable fruit yields. data indicated that, low radiation doses 50,75 and 100 gray increased all morphological and chemical characteristics of the plantlets, plant and fruit of strawberry, whereas radiation doses higher than 100 gray decreased them significantly. moreover 350 and gray were lethal doses. radiation dose 50 gray increased the survival percentage and the length of plantlets by 1.5% and 50% respectively more than the unirradiated treatment in all multiplication stages

  14. Strawberry Achenes Are an Important Source of Bioactive Compounds for Human Health

    Directory of Open Access Journals (Sweden)

    María Teresa Ariza

    2016-07-01

    Full Text Available Strawberries are highly appreciated for their taste, nutritional value and antioxidant compounds, mainly phenolics. Fruit antioxidants derive from achenes and flesh, but achene contribution to the total fruit antioxidant capacity and to the bioaccessibility after intake is still unknown. In this work, the content of total phenolic compounds, flavonoids, anthocyanins and antioxidant capacity (TEAC, FRAP and DPPH of achenes and flesh were compared in non-digested as well as in gastric and intestinal extracts after in vitro digestion. Results showed that, despite strawberry achenes represent a small fraction of the fruit, their contribution to total fruit antioxidant content was more than 41% and accounted for 81% of antioxidant capacity (TEAC. Achenes have higher quantity and different quality of antioxidants in non-digested and digested extracts. Antioxidant release was higher in the in vitro gastric digested extracts, but digestion conditions did not only affect quantity but quality, resulting in differences in antioxidant capacity and highlighting the importance of simulating physiological-like extraction conditions for assessing fruit antioxidant properties on human health. These results give new insights into the use of strawberry achenes as a source of bioactive compounds to be considered in strawberry breeding programs for improving human health.

  15. Strawberry Achenes Are an Important Source of Bioactive Compounds for Human Health

    Science.gov (United States)

    Ariza, María Teresa; Reboredo-Rodríguez, Patricia; Mazzoni, Luca; Forbes-Hernández, Tamara Yuliett; Giampieri, Francesca; Afrin, Sadia; Gasparrini, Massimiliano; Soria, Carmen; Martínez-Ferri, Elsa; Battino, Maurizio; Mezzetti, Bruno

    2016-01-01

    Strawberries are highly appreciated for their taste, nutritional value and antioxidant compounds, mainly phenolics. Fruit antioxidants derive from achenes and flesh, but achene contribution to the total fruit antioxidant capacity and to the bioaccessibility after intake is still unknown. In this work, the content of total phenolic compounds, flavonoids, anthocyanins and antioxidant capacity (TEAC, FRAP and DPPH) of achenes and flesh were compared in non-digested as well as in gastric and intestinal extracts after in vitro digestion. Results showed that, despite strawberry achenes represent a small fraction of the fruit, their contribution to total fruit antioxidant content was more than 41% and accounted for 81% of antioxidant capacity (TEAC). Achenes have higher quantity and different quality of antioxidants in non-digested and digested extracts. Antioxidant release was higher in the in vitro gastric digested extracts, but digestion conditions did not only affect quantity but quality, resulting in differences in antioxidant capacity and highlighting the importance of simulating physiological-like extraction conditions for assessing fruit antioxidant properties on human health. These results give new insights into the use of strawberry achenes as a source of bioactive compounds to be considered in strawberry breeding programs for improving human health. PMID:27409612

  16. Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality

    Directory of Open Access Journals (Sweden)

    Francesca Giampieri

    2014-06-01

    Full Text Available Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.

  17. Polyphenol-rich strawberry extract protects human dermal fibroblasts against hydrogen peroxide oxidative damage and improves mitochondrial functionality.

    Science.gov (United States)

    Giampieri, Francesca; Alvarez-Suarez, José M; Mazzoni, Luca; Forbes-Hernandez, Tamara Y; Gasparrini, Massimiliano; Gonzàlez-Paramàs, Ana M; Santos-Buelga, Celestino; Quiles, José L; Bompadre, Stefano; Mezzetti, Bruno; Battino, Maurizio

    2014-06-11

    Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.

  18. Developments and trends in fruit bar production and characterization.

    Science.gov (United States)

    Orrego, C E; Salgado, N; Botero, C A

    2014-01-01

    Fruits serve as a source of energy, vitamins, minerals, and dietary fiber. One of the barriers in increasing fruit and vegetables consumption is time required to prepare them. Overall, fruit bars have a far greater nutritional value than the fresh fruits because all nutrients are concentrated and, therefore, would be a convenience food assortment to benefit from the health benefits of fruits. The consumers prefer fruit bars that are more tasted followed by proper textural features that could be obtained by establishing the equilibrium of ingredients, the proper choosing of manufacturing stages and the control of the product final moisture content. Fruit bar preparations may include a mixture of pulps, fresh or dried fruit, sugar, binders, and a variety of minor ingredients. Additionally to the conventional steps of manufacturing (pulping, homogenizing, heating, concentrating, and drying) there have been proposed the use of gelled fruit matrices, dried gels or sponges, and extruders as new trends for processing fruit bars. Different single-type dehydration or combined methods include, in order of increasing process time, air-infrared, vacuum and vacuum-microwave drying convective-solar drying, convective drying, and freeze drying are also suggested as alternative to solar traditional drying stage. The dehydration methods that use vacuum exhibited not only higher retention of antioxidants but also better color, texture, and rehydration capacity. Antioxidant activity resulting from the presence of phenolic compounds in the bars is well established. Besides this, fruit bars are also important sources of carbohydrates and minerals. Given the wide range of bioactive factors in fresh fruits that are preserved in fruit bars, it is plausible that their uptake consumption have a positive effect in reducing the risk of many diseases.

  19. Use of low-dose UV-C irradiation to control powdery mildew caused by Podosphaera aphanis on strawberry plants

    Science.gov (United States)

    Powdery mildew of strawberry plants, caused by Podoshaera aphanis, can cause severe losses by reducing fruit yield, quality and predisposing fruit to other diseases. Fungicides have been routinely used to control this disease. However, limitations mainly related to their effectiveness, re-entry pe...

  20. Suitability of Dukat strawberries for studying effects on shelf life of irradiation combined with cold storage

    International Nuclear Information System (INIS)

    Zegota, H.

    1988-01-01

    A new Dukat variety of strawberries was used to study the effect of irradiation combined with cold storage on their shelf life and chemical composition. Strawberries, with or without stems, were irradiated with a dose of 2.5 or 3.0 kGy within 6-10 or 20-24 h after harvesting. Results of the sensory evaluation showed that the minimum storage time for the fruits could be extended by a minimum of 9 days. If the time between harvest and irradiation was shorter, better results for storage experiments were obtained. Fruits with stems were more suitable for cold storage after irradiation than those without stems. Irradiation of strawberries did not change the titratable acidity and content of the reducing sugars. Colour intensity and ascorbic acid levels decreased in proportion to the absorbed dose and storage time. (orig.)

  1. The effect of irradiation on the macro- and microstructure of strawberries

    International Nuclear Information System (INIS)

    Voeroes, Zsuzsanna; Kovacs, Etelka; Zackel, Erna

    1985-01-01

    Samples of Gorella, Pocahontas and Rival strawberry varieties were irradiated (2,5 kGy) by means of a sup(60)Co source to increase storage life. The packaged fruits were stored in cabinets of 80-90% relative vapour content at 5 and 10 deg C, respectively. No significant differences could be found in the refraction % and pH values of the irradiated and control samples and of the packeged and unpackaged samples. Due to irradiation the consistency of fruits softened. At the measurements of strawberries with Instron consistometer the optimal quality marks seem to be obtainable by measuring the deformation steadiness. By means of optical microscopy it was stated that the tissue structure of various strawberry varieties did not alter essentially due to irradiation. (author)

  2. Fruit development of the diploid kiwifruit, Actinidia chinensis 'Hort16A'

    Directory of Open Access Journals (Sweden)

    Richardson Annette C

    2011-12-01

    Full Text Available Abstract Background With the advent of high throughput genomic tools, it is now possible to undertake detailed molecular studies of individual species outside traditional model organisms. Combined with a good understanding of physiological processes, these tools allow researchers to explore natural diversity, giving a better understanding of biological mechanisms. Here a detailed study of fruit development from anthesis through to fruit senescence is presented for a non-model organism, kiwifruit, Actinidia chinensis ('Hort16A'. Results Consistent with previous studies, it was found that many aspects of fruit morphology, growth and development are similar to those of the model fruit tomato, except for a striking difference in fruit ripening progression. The early stages of fruit ripening occur as the fruit is still growing, and many ripening events are not associated with autocatalytic ethylene production (historically associated with respiratory climacteric. Autocatalytic ethylene is produced late in the ripening process as the fruit begins to senesce. Conclusion By aligning A. chinensis fruit development to a phenological scale, this study provides a reference framework for subsequent physiological and genomic studies, and will allow cross comparison across fruit species, leading to a greater understanding of the diversity of fruits found across the plant kingdom.

  3. Strawberry radurisation on a commercial scale

    International Nuclear Information System (INIS)

    Du Venhage, C.A.

    1985-01-01

    An international breakthrough was made at Pelindaba in 1981 when laboratory experiments were adjusted to accomodate commercial quantities of strawberries. Theory and practise of the radurisation of strawberries become compatible and led to the successful marketing of 1,6 million punnets of radurised strawberries during the period 1981 to 1984. Several benefits are derived from this technology, making the marketing and distribution of radurised strawberries an accepted and on-going process

  4. Development and Utilization of Technology on Indian Mango Fruit Processing

    Directory of Open Access Journals (Sweden)

    Rosenda A. Bronce

    2015-11-01

    Full Text Available This project aimed to develop and utilize technology on Indian mango fruit processing. Chemical properties of matured unripe and ripe Indian mangoes were determined in terms of total sugar, reducing sugar, starch, titratable acidity and pH. Fermentation parameters investigated in the study were amount of sugar added (20 and 25% fermentation medium, acidity of fermentation medium (addition of 1.33 and 1.66 grams of citric acid for ripe and dilution of water for unripe, degree of ripening of Indian mango fruits (ripe and unripe and ageing period (3 and 4 months. Sixteen treatments were done in triplicates and a composite sample was taken from each treatment for sensory evaluation. Results of the preference test were subjected to statistical analysis. The physicochemical properties of Indian mango wine produced using best fermentation parameters were determined. Appropriate packaging material was selected and packaging design was developed for Indian mango wine. Project cooperators were selected and the technology was transferred through training and production runs. Results of preference test showed that the wine with best sensory properties was prepared using matured unripe Indian mango diluted with water and added with 25% sugar. According to the panel of sensory experts, the taste of Indian mango wine was strong with proper blending of sweetness and sourness, its mouth feel was smooth and good balance, aroma was hot pungent and its color and appearance was clear and light yellow. Its titratable acidity was 0.622%, pH was 5, alcohol content was 11% and brix was 5°.

  5. Comportamento pós-colheita de frutos de morangueiro após a aplicação pré-colheita de quitosana e acibenzolar-S-metil Post harvest behavior of strawberry fruits after pre harvest treatment with chitosan and acibenzolar-S-methyl

    Directory of Open Access Journals (Sweden)

    Sergio Miguel Mazaro

    2008-03-01

    Full Text Available O uso de indutores de resistência em plantas representa um método alternativo e promissor no controle de podridões pós-colheita de frutos. Assim sendo, foi conduzido um experimento na Universidade Tecnológica Federal do Paraná - Câmpus Dois Vizinhos - PR, no ano de 2004, com objetivo de avaliar a aplicação pré-colheita de quitosana e acibenzolar-S-metil (ASM sobre o comportamento pós-colheita de frutos de morangueiro da cultivar Aromas. O efeito da aplicação de quitosana (0,5; 1,0 e 2,0% e de uma dose de ASM (0,0025% foi verificado em relação à testemunha (água destilada e ao tratamento-controle com aplicação de fungicidas. A aplicação na pré-colheita de quitosana, nas três doses avaliadas, retardou a maturação dos frutos, manteve maior firmeza de polpa e acidez titulável e diminuiu a perda de massa. Esses tratamentos também diminuíram a produção de etileno, o teor de açúcares redutores e mantiveram mais elevado os teores de polifenóis totais. A aplicação de quitosana, nas três doses avaliadas, induziu maior resistência das plantas a patógenos, resultando na diminuição de podridão dos frutos em pós-colheita. No entanto, a concentração de 2% de quitosana causou dano aos frutos na pós-colheita, elevando a taxa respiratória e o teor de açúcares redutores. O ASM teve efeito na retenção da acidez titulável e na redução de podridões, similar ao tratamento com fungicidas. A aplicação de quitosana e ASM na pré-colheita não interferiu na qualidade organoléptica dos frutos em pós-colheita.The use of inductors of resistance in plants represents an alternative and a promising method to control fruit rots at post harvest. The experiment was carried out at Federal Technological University of Paraná - Dois Vizinhos - Campus, during 2004 with the objective to evaluate the induction of chitosan resistance and acibenzolar-S-methyl (ASM on strawberry plants. The treatments compared the effect of chitosan

  6. Screening strawberry plants for anthracnose disease resistance using traditional and molecular techniques

    Science.gov (United States)

    Anthracnose is one of the most destructive diseases of strawberry which may cause fruit rot, leaf and petiole lesions, crown rot, wilt, and death. Crop loss due to anthracnose diseases can reach into the millions of dollars. Three species of Colletotrichum are considered causative agents of anthr...

  7. Influence of gamma-irradiation on the total volatile acids content in strawberries

    International Nuclear Information System (INIS)

    Curzio, O.A.; Piccini, J.L.; Quaranta, H.O.; Perez, S.

    1983-01-01

    The aim of this work was to determine if there exist some kind of correlation between the evolution of the organoleptic characteristics of control and irradiated strawberry and the measured volatile acids content. Affirmative results would suggest that the V.A. content really corresponds to a quality index of the fruit. (orig./AJ)

  8. Identifying Key Flavors in Strawberries Driving Liking via Internal and External Preference Mapping.

    Science.gov (United States)

    Oliver, Penelope; Cicerale, Sara; Pang, Edwin; Keast, Russell

    2018-04-01

    Australian consumers desire the development of a more flavorsome Australian strawberry cultivar. To aid in the development of well-liked strawberries, the attributes driving liking need to be identified. The objective of this research is to apply Preference Mapping (PM) techniques to the descriptive profile of commercial and newly bred strawberry cultivars, together with consumer preference data to determine the flavors contributing to liking. A trained sensory panel (n = 12) used Quantitative Descriptive Analysis (QDA®) methodology to evaluate two appearance, seven aroma, five texture, 10 flavor and 10 aftertaste attributes of three commercial strawberry cultivars and six elite breeding lines grown in Victoria, Australia. Strawberry consumers (n = 150) assessed their liking of the same strawberry cultivars. QDA® significantly discriminated strawberries on 28 of the 34 sensory attributes. There were significant differences in hedonic ratings of strawberries (F(8,714) = 11.5, P = 0.0001), with Hierarchical Cluster Analysis (HCA) identifying three consumer clusters each displaying differing patterns of preference. Internal and external PM techniques were applied to the data to identify the attributes driving consumer acceptability. Sweet, berry, caramel, fruity and floral attributes were identified as most contributing to liking. Sour, citrus, green, astringent, firm and gritty attributes were conversely associated with a reduction in consumer liking. Elite Lines 2 and 6 have been identified as having the broadest appeal, satisfying between 60% and 70% of consumers in the population assessed, thus the introduction of these cultivars should satisfy the largest group of consumers in the Australian market. The results of this research could be applied to breeding programs, to ensure newly bred cultivars express characteristics that were identified as well-liked amongst consumers. In addition, this research provides evidence for marketing strawberries by

  9. Phytochemicals and Medicinal Properties of Indigenous Tropical Fruits with Potential for Commercial Development

    Directory of Open Access Journals (Sweden)

    Hock Eng Khoo

    2016-01-01

    Full Text Available Hundreds of fruit-bearing trees are native to Southeast Asia, but many of them are considered as indigenous or underutilized. These species can be categorized as indigenous tropical fruits with potential for commercial development and those possible for commercial development. Many of these fruits are considered as underutilized unless the commercialization is being realized despite the fact that they have the developmental potential. This review discusses seven indigenous tropical fruits from 15 species that have been identified, in which their fruits are having potential for commercial development. As they are not as popular as the commercially available fruits, limited information is found. This paper is the first initiative to provide information on the phytochemicals and potential medicinal uses of these fruits. Phytochemicals detected in these fruits are mainly the phenolic compounds, carotenoids, and other terpenoids. Most of these phytochemicals are potent antioxidants and have corresponded to the free radical scavenging activities and other biological activities of the fruits. The scientific research that covered a broad range of in vitro to in vivo studies on the medicinal potentials of these fruits is also discussed in detail. The current review is an update for researchers to have a better understanding of the species, which simultaneously can provide awareness to enhance their commercial value and promote their utilization for better biodiversity conservation.

  10. Strawberry polyphenols attenuate ethanol-induced gastric lesions in rats by activation of antioxidant enzymes and attenuation of MDA increase.

    Directory of Open Access Journals (Sweden)

    José M Alvarez-Suarez

    Full Text Available BACKGROUND AND AIM: Free radicals are implicated in the aetiology of gastrointestinal disorders such as gastric ulcer, colorectal cancer and inflammatory bowel disease. Strawberries are common and important fruit due to their high content of essential nutrient and beneficial phytochemicals which seem to have relevant biological activity on human health. In the present study we investigated the antioxidant and protective effects of three strawberry extracts against ethanol-induced gastric mucosa damage in an experimental in vivo model and to test whether strawberry extracts affect antioxidant enzyme activities in gastric mucosa. METHODS/PRINCIPAL FINDINGS: Strawberry extracts were obtained from Adria, Sveva and Alba cultivars. Total antioxidant capacity and radical scavenging capacity were performed by TEAC, ORAC and electron paramagnetic resonance assays. Identification and quantification of anthocyanins was carried out by HPLC-DAD-MS analyses. Different groups of animals received 40 mg/day/kg body weight of strawberry crude extracts for 10 days. Gastric damage was induced by ethanol. The ulcer index was calculated together with the determination of catalase and SOD activities and MDA contents. Strawberry extracts are rich in anthocyanins and present important antioxidant capacity. Ethanol caused severe gastric damage and strawberry consumption protected against its deleterious role. Antioxidant enzyme activities increased significantly after strawberry extract intake and a concomitantly decrease in gastric lipid peroxidation was found. A significant correlation between total anthocyanin content and percent of inhibition of ulcer index was also found. CONCLUSIONS: Strawberry extracts prevented exogenous ethanol-induced damage to rats' gastric mucosa. These effects seem to be associated with the antioxidant activity and phenolic content in the extract as well as with the capacity of promoting the action of antioxidant enzymes. A diet rich in

  11. Strawberry Polyphenols Attenuate Ethanol-Induced Gastric Lesions in Rats by Activation of Antioxidant Enzymes and Attenuation of MDA Increase

    Science.gov (United States)

    Alvarez-Suarez, José M.; Dekanski, Dragana; Ristić, Slavica; Radonjić, Nevena V.; Petronijević, Nataša D.; Giampieri, Francesca; Astolfi, Paola; González-Paramás, Ana M.; Santos-Buelga, Celestino; Tulipani, Sara; Quiles, José L.; Mezzetti, Bruno; Battino, Maurizio

    2011-01-01

    Background and Aim Free radicals are implicated in the aetiology of gastrointestinal disorders such as gastric ulcer, colorectal cancer and inflammatory bowel disease. Strawberries are common and important fruit due to their high content of essential nutrient and beneficial phytochemicals which seem to have relevant biological activity on human health. In the present study we investigated the antioxidant and protective effects of three strawberry extracts against ethanol-induced gastric mucosa damage in an experimental in vivo model and to test whether strawberry extracts affect antioxidant enzyme activities in gastric mucosa. Methods/Principal Findings Strawberry extracts were obtained from Adria, Sveva and Alba cultivars. Total antioxidant capacity and radical scavenging capacity were performed by TEAC, ORAC and electron paramagnetic resonance assays. Identification and quantification of anthocyanins was carried out by HPLC-DAD-MS analyses. Different groups of animals received 40 mg/day/kg body weight of strawberry crude extracts for 10 days. Gastric damage was induced by ethanol. The ulcer index was calculated together with the determination of catalase and SOD activities and MDA contents. Strawberry extracts are rich in anthocyanins and present important antioxidant capacity. Ethanol caused severe gastric damage and strawberry consumption protected against its deleterious role. Antioxidant enzyme activities increased significantly after strawberry extract intake and a concomitantly decrease in gastric lipid peroxidation was found. A significant correlation between total anthocyanin content and percent of inhibition of ulcer index was also found. Conclusions Strawberry extracts prevented exogenous ethanol-induced damage to rats' gastric mucosa. These effects seem to be associated with the antioxidant activity and phenolic content in the extract as well as with the capacity of promoting the action of antioxidant enzymes. A diet rich in strawberries might exert a

  12. An anthocyanin rich strawberry extract induces apoptosis and ROS while decreases glycolysis and fibrosis in human uterine leiomyoma cells.

    Science.gov (United States)

    Islam, Md Soriful; Giampieri, Francesca; Janjusevic, Milijana; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y; Mazzoni, Luca; Greco, Stefania; Giannubilo, Stefano Raffaele; Ciavattini, Andrea; Mezzetti, Bruno; Capocasa, Franco; Castellucci, Mario; Battino, Maurizio; Ciarmela, Pasquapina

    2017-04-04

    Uterine leiomyomas are highly prevalent benign tumors in reproductive aged women. Unfortunately, medical treatments are still limited and no preventive therapies have been developed. In the present study, we investigated the therapeutic effects of strawberry extract on uterine leiomyoma cells. Leiomyoma and myometrial cells were treated with strawberry (cultivar Alba) extract (250 μg/ml) for 48 h to measure apoptosis, reactive oxygen species (ROS), oxidative phosphorylation (OCR, oxygen consumption rate) and glycolysis (ECAR, extracellular acidification rate) as well as fibrosis associated gene and/or protein expression. In leiomyoma cells, strawberry increased the percentage of apoptotic and dead cells. Strawberry significantly increased ROS concentration in leiomyoma cells, while decreased it in myometrial cells. After strawberry treatment, leiomyoma cells showed a significant decreased rate of ECAR, while OCR was unchanged in both myometrial and leiomyoma cells. Strawberry significantly decreased collagen1A1, fibronectin and versican mRNA expression in leiomyoma cells. The reduced protein expression of fibronectin was observed by strawberry extract in leiomyoma cells as well. Furthermore, strawberry was able to reduce activin A induced fibronectin, collagen1A1, and versican as well as activin A and PAI-1 mRNA expression in leiomyoma cells. This study suggests that strawberry can be developed as therapeutic and/or preventive agent for uterine leiomyomas.

  13. BEL1-LIKE HOMEODOMAIN 11 regulated chloroplast development and chlorophyll synthesis in tomato fruit

    Science.gov (United States)

    Chloroplast development and chlorophyll content and metabolism in unripe tomato contribute to the growth and development of the fruit, and also the ripe fruit quality, but the mechanism is poorly understood. In this work, seven homeobox-containing transcription factors (TFs) with specific ripening-a...

  14. Fruit, seed and embryo development of different cassava (Manihot ...

    African Journals Online (AJOL)

    SAM

    2014-03-24

    Mar 24, 2014 ... Key words: Manihot esculenta Crantz, day after pollination (DAP), fruit set, seed size, .... (day/night) photoperiod with light supplied by white fluorescent tubes .... Science Foundation of China (31301378) and the projects.

  15. Optimization of strawberry disinfection by fogging of a mixture of peracetic acid and hydrogen peroxide based on microbial reduction, color and phytochemicals retention.

    Science.gov (United States)

    Van de Velde, Franco; Vaccari, María Celia; Piagentini, Andrea Marcela; Pirovani, María Élida

    2016-09-01

    The fogging of strawberries using a environmentally friendly sanitizer mixture of peracetic acid (5%) and hydrogen peroxide (20%) was performed in a model chamber and modeled as a function of the concentration (3.4, 20.0, 60.0, 100.0 and 116.6 µL sanitizer L(-) (1) air chamber) and the treatment time (5.7, 15.0, 37.5, 60.0 and 69.3 min). The sanitizer fogging was adequate for reducing total mesophilic microbial and yeasts and moulds counts of fruits until seven days of storage at 2℃. However, sanitizer oxidant properties adversely affected the content of total anthocyanins, total phenolics, vitamin C, and antioxidant capacity to various degrees, with some deleterious changes in the fruits color, depending on the fogging conditions. A multiple numeric response optimization was developed based on 2.0 log microbiological reduction, maximum phytochemicals and antioxidant capacity retentions, with no changes in the fruits color, being the optimal fogging conditions achieved: 10.1 µL sanitizer L(-1) air chamber and 29.6 min. The fogging of strawberries at these conditions may represent a promising postharvest treatment option for extending their shelf-life without affecting their sensory quality and bioactive properties. © The Author(s) 2016.

  16. Irradiation preservation processing study of strawberry

    International Nuclear Information System (INIS)

    Xu Shihong; Zhu Zhaodi

    1992-01-01

    Radiation preservation processing of strawberry was studied. The results show that single seal processing or combining with irradiation processing under given doses is of no practical value, but only combining with chill processing can promote lengthening period of fresh keeping. Single irradiation processing under given doses provides not only good initial state, but also the beginning of bad development at top speed on microorganism. The composite processing of irradiation, chill and seal can supply extended shelf lifetime with optimum value of dual synergetic effect

  17. An inbred line of the diploid strawberry Fragaria vesca f. semperflorens for genomic and molecular genetic studies in the Rosaceae.

    Science.gov (United States)

    Slovin, Janet P; Schmitt, Kyle; Folta, Kevin M

    2009-10-31

    The diploid woodland strawberry (Fragaria vesca) is an attractive system for functional genomics studies. Its small stature, fast regeneration time, efficient transformability and small genome size, together with substantial EST and genomic sequence resources make it an ideal reference plant for Fragaria and other herbaceous perennials. Most importantly, this species shares gene sequence similarity and genomic microcolinearity with other members of the Rosaceae family, including large-statured tree crops (such as apple, peach and cherry), and brambles and roses as well as with the cultivated octoploid strawberry, F. xananassa. F. vesca may be used to quickly address questions of gene function relevant to these valuable crop species. Although some F. vesca lines have been shown to be substantially homozygous, in our hands plants in purportedly homozygous populations exhibited a range of morphological and physiological variation, confounding phenotypic analyses. We also found the genotype of a named variety, thought to be well-characterized and even sold commercially, to be in question. An easy to grow, standardized, inbred diploid Fragaria line with documented genotype that is available to all members of the research community will facilitate comparison of results among laboratories and provide the research community with a necessary tool for functionally testing the large amount of sequence data that will soon be available for peach, apple, and strawberry. A highly inbred line, YW5AF7, of a diploid strawberry Fragaria vesca f. semperflorens line called "Yellow Wonder" (Y2) was developed and examined. Botanical descriptors were assessed for morphological characterization of this genotype. The plant line was found to be rapidly transformable using established techniques and media formulations. The development of the documented YW5AF7 line provides an important tool for Rosaceae functional genomic analyses. These day-neutral plants have a small genome, a seed to seed

  18. An inbred line of the diploid strawberry Fragaria vesca f. semperflorens for genomic and molecular genetic studies in the Rosaceae

    Directory of Open Access Journals (Sweden)

    Folta Kevin M

    2009-10-01

    Full Text Available Abstract Background The diploid woodland strawberry (Fragaria vesca is an attractive system for functional genomics studies. Its small stature, fast regeneration time, efficient transformability and small genome size, together with substantial EST and genomic sequence resources make it an ideal reference plant for Fragaria and other herbaceous perennials. Most importantly, this species shares gene sequence similarity and genomic microcolinearity with other members of the Rosaceae family, including large-statured tree crops (such as apple, peach and cherry, and brambles and roses as well as with the cultivated octoploid strawberry, F. ×ananassa. F. vesca may be used to quickly address questions of gene function relevant to these valuable crop species. Although some F. vesca lines have been shown to be substantially homozygous, in our hands plants in purportedly homozygous populations exhibited a range of morphological and physiological variation, confounding phenotypic analyses. We also found the genotype of a named variety, thought to be well-characterized and even sold commercially, to be in question. An easy to grow, standardized, inbred diploid Fragaria line with documented genotype that is available to all members of the research community will facilitate comparison of results among laboratories and provide the research community with a necessary tool for functionally testing the large amount of sequence data that will soon be available for peach, apple, and strawberry. Results A highly inbred line, YW5AF7, of a diploid strawberry Fragaria vesca f. semperflorens line called "Yellow Wonder" (Y2 was developed and examined. Botanical descriptors were assessed for morphological characterization of this genotype. The plant line was found to be rapidly transformable using established techniques and media formulations. Conclusion The development of the documented YW5AF7 line provides an important tool for Rosaceae functional genomic analyses

  19. Investment in plant research and development bears fruit in China.

    Science.gov (United States)

    Chong, Kang; Xu, Zhihong

    2014-04-01

    Recent rapid progress in plant science and biotechnology in China demonstrates that China's stronger support for funding in plant research and development (R&D) has borne fruit. Chinese groups have contributed major advances in a range of fields, such as rice biology, plant hormone and developmental biology, genomics and evolution, plant genetics and epigenetics, as well as plant biotechnology. Strigolactone studies including those identifying its receptor and dissecting its complex structure and signaling are representative of the recent researches from China at the forefront of the field. These advances are attributable in large part to interdisciplinary studies among scientists from plant science, chemistry, bioinformatics, structural biology, and agronomy. The platforms provided by national facilities facilitate this collaboration. As well, efficient restructuring of the top-down organization of state programs and free exploration of scientists' interests have accelerated achievements by Chinese researchers. Here, we provide a general outline of China's progress in plant R&D to highlight fields in which Chinese research has made significant contributions.

  20. A comprehensive approach to evaluate the freshness of strawberries and carrots

    DEFF Research Database (Denmark)

    Peneau, S.; Brockhoff, Per B.; Escher, F.

    2007-01-01

    of strawberries and carrots that varied in cultivar, as well as with time and conditions of storage. Product characteristics measured by descriptive sensory and physico-chemical analyses were related to consumer and expert panel (individual and consensus) ratings of freshness. Results showed that a large number...... of attributes contributed to the freshness of strawberries and carrots and that those were also indicators of the physiological ageing of these products. Our results suggest that consumer evaluation of fruit and vegetable freshness corresponds to an evaluation of this ageing process through the observation...

  1. Influences of the GAMMA radiation on useful life of strawberry

    International Nuclear Information System (INIS)

    Bonilla B, Ligia M.; Sepulveda P, Claudia M.

    1993-01-01

    The present investigation was carried out with the purpose of prolonging the useful life of strawberry (bird variety) by means of gamma radiation, eliminating the causing microorganisms of the deterioration of the fruit partially: aerobic mesophyll, psicrotrophos, molds and yeasts, effect that was controlled by means of the recount of the same ones. In the same way, it was carried out a quantitative analysis of some parameters such organolitics as flavor, color and hardness, to observe the behavior of the strawberry with the different applied treatments. The irradiation process was made with a source of Co-60, provided by the INEA with an activity of 82 kilo curios. The used radiation doses were 0, 1.0, 1.5, 2.0, 2.5 and 3.0 kGy, which were quantified by means of chemical dosimeter : Sulfate cupric ferrous-sulfate. Strawberries were storage under refrigeration with controlled conditions of temperature between 1 and 2 C and relative humidity of 85-90, and periodically, samples were removed for different analyses

  2. Induced Mutation in Strawberry by Using Gamma Radiation

    International Nuclear Information System (INIS)

    Phadvibulya, Valailak; Puripanyavanich, Vichai; Pipattanawongse, Narongchai; Yeesawasdi, Voravit; Teja, Waich

    2003-12-01

    The culture of strawberry plants variety Tio ga were irradiated with gamma rays at doses of 0, 20, 40, 60, 80, 100,120 and 140 Gy. They were transferred to the MS medium supplemented with BA 0.1 mg/l. It was found that radiation dose which caused 50% lethality (LD 50 ) was 80 Gy. The survival plants were transferred to rooting medium (MS medium) and then transplanted to field for stolon production and yield investigation. Two lines with good fruit quality were obtained after selection till M 1 V 5 . They were planted for further testing. In experiment II, The culture of strawberry plants varieties Royal 20, Royal 70, SM and Dover were irradiated with gamma rays at doses of 0, 40 and 60 Gy. They were transplanted to field for stolon and yield production. Three lines from Royal 70 were obtained after selection till M 1 V 4 . In experiment III, the culture of strawberry plants varieties Royal 20, Royal 50, Royal 70, Nyoho and Selva were irradiated with gamma rays at the same doses as experiment II. Nine lines from Royal 70 and four lines from Nyoho with good characters were selected for further testing

  3. Biological control of tortricids and aphids in strawberries

    DEFF Research Database (Denmark)

    Sigsgaard, Lene; Enkegaard, Annie; Eilenberg, Jørgen

    Cropping practice and biological control can contribute to reduced pesticide use in strawberries. Organic strawberries are less attacked by strawberry tortricid and buckwheat flower strips can augment its natural enemies. Against shallot aphid the two-spot ladybird is promising....

  4. Application of thermoluminescence measurements to detect irradiated strawberries

    International Nuclear Information System (INIS)

    Heide, L.; Guggenberger, R.; Bogl, K.W.

    1990-01-01

    The thermoluminescence intensity of unirradiated and irradiated strawberries was studied with regard to dose response, storage time, and different varieties. An identification method could finally be developed. Further investigations were carried out to determine the origin of the thermoluminescence effect, which was found to be attributable to mineral grains adhering to the sample surface

  5. Comparative Transcriptome Analysis of Cultivated and Wild Watermelon during Fruit Development.

    Science.gov (United States)

    Guo, Shaogui; Sun, Honghe; Zhang, Haiying; Liu, Jingan; Ren, Yi; Gong, Guoyi; Jiao, Chen; Zheng, Yi; Yang, Wencai; Fei, Zhangjun; Xu, Yong

    2015-01-01

    Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an important vegetable crop world-wide. Watermelon fruit quality is a complex trait determined by various factors such as sugar content, flesh color and flesh texture. Fruit quality and developmental process of cultivated and wild watermelon are highly different. To systematically understand the molecular basis of these differences, we compared transcriptome profiles of fruit tissues of cultivated watermelon 97103 and wild watermelon PI296341-FR. We identified 2,452, 826 and 322 differentially expressed genes in cultivated flesh, cultivated mesocarp and wild flesh, respectively, during fruit development. Gene ontology enrichment analysis of these genes indicated that biological processes and metabolic pathways related to fruit quality such as sweetness and flavor were significantly changed only in the flesh of 97103 during fruit development, while those related to abiotic stress response were changed mainly in the flesh of PI296341-FR. Our comparative transcriptome profiling analysis identified critical genes potentially involved in controlling fruit quality traits including α-galactosidase, invertase, UDP-galactose/glucose pyrophosphorylase and sugar transporter genes involved in the determination of fruit sugar content, phytoene synthase, β-carotene hydroxylase, 9-cis-epoxycarotenoid dioxygenase and carotenoid cleavage dioxygenase genes involved in carotenoid metabolism, and 4-coumarate:coenzyme A ligase, cellulose synthase, pectinesterase, pectinesterase inhibitor, polygalacturonase inhibitor and α-mannosidase genes involved in the regulation of flesh texture. In addition, we found that genes in the ethylene biosynthesis and signaling pathway including ACC oxidase, ethylene receptor and ethylene responsive factor showed highly ripening-associated expression patterns, indicating a possible role of ethylene in fruit development and ripening of watermelon, a non-climacteric fruit. Our analysis provides

  6. Development of a method based on on-line reversed phase liquid chromatography and gas chromatography coupled by means of an adsorption-desorption interface for the analysis of selected chiral volatile compounds in methyl jasmonate treated strawberries.

    Science.gov (United States)

    de la Peña Moreno, Fernando; Blanch, Gracia Patricia; Flores, Gema; Ruiz Del Castillo, Maria Luisa

    2010-02-12

    A method based on the use of the through oven transfer adsorption-desorption (TOTAD) interface in on-line coupling between reversed phase liquid chromatography and gas chromatography (RPLC-GC) for the determination of chiral volatile compounds was developed. In particular, the method was applied to the study of the influence of methyl jasmonate (MJ) treatment on the production and enantiomeric composition of selected aroma compounds in strawberry. The compounds studied were ethyl 2-methylbutanoate, linalool and 4-hydroxy-2,5-dimethyl-3(2H)-furanone (i.e. furaneol), which were examined on days 3, 6 and 9 after treatment. The method developed resulted in relative standard deviations (RSDs) of 21.6%, 8.1% and 9.8% and limits of detection (LD) of 0.04, 0.07 and 0.02mg/l for ethyl 2-methylbutanoate, linalool and furaneol, respectively. The application of the RPLC-TOTAD-GC method allowed higher levels of ethyl 2-methylbutanoate, linalool and furaneol to be detected, particularly after 9 days of treatment. Besides, MJ demonstrated to affect the enantiomeric distribution of ethyl 2-methylbutanoate. On the contrary, the enantiomeric composition of linalool and furaneol kept constant in both control and MJ-treated strawberries throughout the study. These results are discussed. Copyright 2009 Elsevier B.V. All rights reserved.

  7. Comparison of physiochemical changes in two popular strawberry cultivars grown in Iran (cvs. Kurdistan & Selva during storage time at 4°C

    Directory of Open Access Journals (Sweden)

    Jouki Mohammad

    2012-01-01

    Full Text Available In this study the effect of storage time on some quality parameters of two strawberry cultivars fruits storage at 4°C have been evaluated. Several quality parameters were monitored during the storage period. The samples were analyzed 1, 7, 14 and 21days during storage. Fruits were analyzed about fruit weight, sugar content, chromatic parameters L*, a*, b*, fruit firmness, total titrable acidity, pH, and sensory evaluation. The Kurdistan strawberries retained their weight throughout the experiment as opposed to the Selva strawberries which lost 0.6% of their weight per day because of dehydration. The Selva cultivar was exceptionally firmer than Kurdistan strawberries. Two cultivars were rich in vitamin C and sugar but these values were more in Kurdistan cultivar. As expected, Chroma decreased and over the storage time in two cultivars. The results of this study showed that packaged Kurdistan strawberries could prove suitable for 7 days at 4°C compare with 4 days for selva strawberries.

  8. Transcription analysis of apple fruit development using cDNA microarrays

    NARCIS (Netherlands)

    Soglio, V.; Costa, F.; Molthoff, J.W.; Weemen-Hendriks, M.; Schouten, H.J.; Gianfranceschi, L.

    2009-01-01

    The knowledge of the molecular mechanisms underlying fruit quality traits is fundamental to devise efficient marker-assisted selection strategies and to improve apple breeding. In this study, cDNA microarray technology was used to identify genes whose expression changes during fruit development and

  9. Evaluation of Appropriate Reference Genes for Gene Expression Normalization during Watermelon Fruit Development.

    Directory of Open Access Journals (Sweden)

    Qiusheng Kong

    Full Text Available Gene expression analysis in watermelon (Citrullus lanatus fruit has drawn considerable attention with the availability of genome sequences to understand the regulatory mechanism of fruit development and to improve its quality. Real-time quantitative reverse-transcription PCR (qRT-PCR is a routine technique for gene expression analysis. However, appropriate reference genes for transcript normalization in watermelon fruits have not been well characterized. The aim of this study was to evaluate the appropriateness of 12 genes for their potential use as reference genes in watermelon fruits. Expression variations of these genes were measured in 48 samples obtained from 12 successive developmental stages of parthenocarpic and fertilized fruits of two watermelon genotypes by using qRT-PCR analysis. Considering the effects of genotype, fruit setting method, and developmental stage, geNorm determined clathrin adaptor complex subunit (ClCAC, β-actin (ClACT, and alpha tubulin 5 (ClTUA5 as the multiple reference genes in watermelon fruit. Furthermore, ClCAC alone or together with SAND family protein (ClSAND was ranked as the single or two best reference genes by NormFinder. By using the top-ranked reference genes to normalize the transcript abundance of phytoene synthase (ClPSY1, a good correlation between lycopene accumulation and ClPSY1 expression pattern was observed in ripening watermelon fruit. These validated reference genes will facilitate the accurate measurement of gene expression in the studies on watermelon fruit biology.

  10. Evaluation of Appropriate Reference Genes for Gene Expression Normalization during Watermelon Fruit Development.

    Science.gov (United States)

    Kong, Qiusheng; Yuan, Jingxian; Gao, Lingyun; Zhao, Liqiang; Cheng, Fei; Huang, Yuan; Bie, Zhilong

    2015-01-01

    Gene expression analysis in watermelon (Citrullus lanatus) fruit has drawn considerable attention with the availability of genome sequences to understand the regulatory mechanism of fruit development and to improve its quality. Real-time quantitative reverse-transcription PCR (qRT-PCR) is a routine technique for gene expression analysis. However, appropriate reference genes for transcript normalization in watermelon fruits have not been well characterized. The aim of this study was to evaluate the appropriateness of 12 genes for their potential use as reference genes in watermelon fruits. Expression variations of these genes were measured in 48 samples obtained from 12 successive developmental stages of parthenocarpic and fertilized fruits of two watermelon genotypes by using qRT-PCR analysis. Considering the effects of genotype, fruit setting method, and developmental stage, geNorm determined clathrin adaptor complex subunit (ClCAC), β-actin (ClACT), and alpha tubulin 5 (ClTUA5) as the multiple reference genes in watermelon fruit. Furthermore, ClCAC alone or together with SAND family protein (ClSAND) was ranked as the single or two best reference genes by NormFinder. By using the top-ranked reference genes to normalize the transcript abundance of phytoene synthase (ClPSY1), a good correlation between lycopene accumulation and ClPSY1 expression pattern was observed in ripening watermelon fruit. These validated reference genes will facilitate the accurate measurement of gene expression in the studies on watermelon fruit biology.

  11. Regularities in the +H14C assimilate supply of peach fruits at their last development stage within the range of a fruit-bearing branchlet

    International Nuclear Information System (INIS)

    Petrov, A.; Manolov, P.

    1977-01-01

    Studies have been accomplished by means of 14 C and autoradiography on the transport link between individual shoots and Redhaven fruits in their advanced development (about a week before maturity) which have formed within the range of a fruit-bearing branchlet. This confirms the conception of directing the photosynthetic prod%cts mainly to the ripening fruits. The basic regularities of the photoassimilate transport prove to be the same as at an early phase of fruit development. Nevertheless, there are also some new traits of morphological and physiological essence such as relatively a large phloem fibre scope of the fruit-bearing branchlet, creating better possibilities of taking over the transport products of the photosynthesis and enhanced attraction opwer of fruits, conditioning a strengthened linear and additional transversal transport, thus contributing to the rapid growth of fruits in their last development phase. Under the influence of these factors, the independence of individual shoots and fruits along and around a fruit-bearing branchlet is substantially less than at an early development phase. (author)

  12. Effect of gamma-irradiation on the storage life elongation of fresh strawberries and raspberries

    International Nuclear Information System (INIS)

    Tencheva, S.; Todorov, S.

    1975-01-01

    The possibility of preserving strawberries and raspberries at room temperature by irradiation is studied. The fruits were packed in perforated plastic bags and irradiated on gamma-device (Cobalt-60) at a dose power of 1900 rad/min. Strawberries were irradiated with 250 krad, raspberries of ''Rubin'' variety with 150 or 200 krad. Irratiated and control fruits were stored at 20sup(o) - 22sup(o)C and examined every day until the first signs of microbial decay appeared. It is found that the total number of microorganisms drops immediately after irradiation. The earliest signs of decay in control strawberries appeared on the second day after they had been packed; in irradiated strawberries - on the fourth or fifth day, with a rapid progress of decay thereafter. Decay of control raspberries started on the first day and by the fourth day all packages were completely moldy, while irradiated fruits had a very good appearance. Single mold spots appeared on the third or fourth day after exposure to 150 krad and later than the fifth day after exposure to 200 krad. Physico-chemical studies disclosed a tendency toward decrease in the sugar content immediately after irradiation. A post-irradiation decrease in the content of total pectine substances and ascorbic acid was also observed. At the end of preservation ascorbic acid in strawberries was reduced by 33 per cent and in raspberries by 19 - 70 per cent. The spectra of antecyonic substances showed that during the preservation of irradiated fruits oxidation has been more intensive. (Ch.K.)

  13. Cloning and expression analysis of FaPR-1 gene in strawberry

    Science.gov (United States)

    Mo, Fan; Luo, Ya; Ge, Cong; Mo, Qin; Ling, Yajie; Luo, Shu; Tang, Haoru

    2018-04-01

    The FaPR-1 gene was cloned by RT-PCR from `Benihoppe' strawberry and its bioinformatics analysis was conducted. The results showed that the open reading frame was 483 bp encoding encoding l60 amino acids which protein molecular weight and theoretical isoelectricity were 17854.17 and 8.72 respectively. Subcellular localization prediction shows that this gene is located extracellularly. By comparing strawberry FaPR-l and other plant Pathogenesis-related protein, homology and phylogenetic tree construction showed that the homology with grapes, peach is relatively close. In the treatments of ABA, sucrose and the mixture of the two, the expression of FaPR-1 in strawberry fruit were significantly increased.

  14. Ethanol, vinegar and Origanum vulgare oil vapour suppress the development of anthracnose rot in tomato fruit.

    Science.gov (United States)

    Tzortzakis, Nikos G

    2010-08-15

    Anthracnose rot (Colletotrichum coccodes) development in vitro or in tomato (Lycopersicon esculentum L.) fruit was evaluated after treatment with absolute ethyl alcohol (AEA), vinegar (VIN), chlorine (CHL) or origanum oil (ORI) and storage at 12 degrees C and 95% relative humidity during or following exposure to the volatiles. Fruit treated with vapours reduced fungal spore germination/production, but in the case of AEA- and VIN-treated fruits, fungal mycelium development was accelerated. Fruit lesion development was suppressed after fruit exposure to pure (100% v/v) AEA or ORI vapours which were accompanied by increased fruit cracking. Exposure to pure VIN-, CHL- and ORI vapours reduced (up to 92%) spore germination in vitro, but no differences were observed in the AEA treatment. The benefits associated with volatiles-enrichment were maintained in fruit pre-exposed to vapours, resulting in suppression in spore germination and spore production. However, studies performed on fungi grown on Potato Dextrose Agar revealed fewer direct effects of volatiles on fungal colony development and spore germination per se, implying that suppression of pathogen development was due in a large part to the impact of volatiles on fruit-pathogen interactions and/or 'memory' effects on fruit tissue. Work is currently focussing on the mechanisms underlying the impacts of volatiles on fruit quality related attributes. The results of this study indicate that volatiles may be considered as an alternative to the traditional postharvest sanitizing techniques. Each commodity needs to be individually assessed, and the volatile concentration and sanitising technique optimised, before the volatile treatment is used commercially. Copyright 2010 Elsevier B.V. All rights reserved.

  15. Species of thrips (Insecta, Thysanoptera in two strawberry production systems in Rio Grande do Sul State, Brazil

    Directory of Open Access Journals (Sweden)

    Silvia M. J. Pinent

    2011-09-01

    Full Text Available Species of thrips (Insecta, Thysanoptera in two strawberry production systems in Rio Grande do Sul, Brazil. Thrips are tiny insects responsible for the reduction of strawberry fruit quality. The work aimed to record and quantify the thysanopterofauna present in two strawberry production systems, low tunnel and semi-hydroponic. Leaves, flowers and fruits were collected weekly, from July 2005 to December 2006 in Caxias do Sul and Bom Princípio municipalities, RS. A total of 664 individuals were collected, representing two families, four genus and 10 species: Frankliniella occidentalis (Pergande, 1895, F. schultzei (Trybom, 1910, F. rodeos Moulton, 1933, F. simplex (Priesner, 1924, F. williamsi (Hood, 1915, F. gemina (Bagnall, 1919, Frankliniella sp., Thrips tabaci (Lindeman, 1888, Thrips tabaci (Lindeman, 1888, Caliothrips fasciatus (Pergande 1895 from Thripidae and Heterothrips sp. from Heterothripidae. Frankliniella occidentalis represented 89.7% of the samples with 95.8% of the species collected in flowers, 3.9% in fruits and 0.8% in leaves. The results show that flowers are the most important food resource for these insects on strawberry plants. Frankliniella rodeos, F. simplex, F. williamsi, C. fasciatus, and Heterothrips sp. are new records on strawberry for Brazil.

  16. Dynamics of sugar-metabolic enzymes and sugars accumulation during watermelon (citrullus lanatus) fruit development

    International Nuclear Information System (INIS)

    Zhang, H.

    2016-01-01

    We analyzed sugar accumulation and the activities of sugar-metabolic enzymes in ripening fruits of three cultivars of watermelon; a high-sugar type w2, a low-sugar type (w1), and their hybrid. In w2, the glucose and fructose contents were higher than the sucrose content in the earlier stage of fruit development, and fruit growth was accompanied by increases in glucose, fructose, and sucrose contents. The sucrose content increased substantially after 20 days after anthesis (DAA) and it was the main soluble sugar in mature fruit (sucrose: hexoses ratio, 0.71). In W, the fructose and glucose contents were significantly higher than the sucrose content in mature fruit (sucrose: hexoses ratio, 0.25). Comparing the two parent cultivars, sucrose was the most important factor affecting the total sugar content in mature fruit, although glucose and fructose also contributed to total sugar contents. The fructose and glucose contents in the fruit of F1 were mid-way between those of their parents, while the sucrose content was closer to that of W (sucrose:hexoses ratio in F1, 0.26). In the early stage of fruit development of W2, the activities of acid invertase and neutral invertase were higher than those of sucrose synthase and sucrose phosphate synthase. After 20 DAA, the acid invertase and neutral invertase activities decreased and those of sucrose synthase and sucrose phosphate synthase increased, leading to increased sucrose content. In W1, the activities of acid invertase and neutral invertase were higher than those of sucrose synthase and sucrose phosphate synthase at the early stage. The sucrose synthase and sucrose phosphate synthase activities were lower in W1 than in W2 at the later stages of fruit development. The patterns of sugar accumulation and sugar-metabolic enzyme activities during fruit development in F1 were similar to those in W1. (author)

  17. Attempts to increase storage stability of strawberry yoghurt by combination treatments

    International Nuclear Information System (INIS)

    Kiss, I.

    1975-01-01

    The aim of the experiments was to establish whether the microbiological stability of strawberry yoghurt might be improved by decreasing the microbial load of the fruit. The effect of heat treatment, freezing, irradiation and various combinations of these treatments upon cell count and sensory quality was investigated. It was established that none of the individual treatments was entirely satisfactory. Surfacial heat treatment at 55 0 C, freezing and irradiation with 0.4-0.6 Mrad substantially increased the storage life of strawberries or that of the yoghurt prepared with this fruit; when compared to yoghurt made with frozen strawberries by the dairy factory, the increase was 2.5 fold at 15 0 C and 3.5 fold at 2 0 C. The relative increase of storage life was lower at lower yeast-cell counts. The strawberries irradiated with doses above 0.2 Mrad showed aroma and flavour changes immediately upon treatment. This effect, however, was eliminated after some days. The yoghurt made with strawberries given a radiation treatment of 0.57 Mrad did not differ organoleptically from the yoghurt made with untreated strawberries. In the knowledge of the survival rate of yeasts after irradiation the D 10 values were established. These were found in the dose range between 0.043 and 0.087 Mrad. It was established that the applied heat treatment, freezing and irradiation at these dose levels and at 10 3 -10 4 cells per gram were not sufficient from the point of view of microbiological stability. (F.J.)

  18. Variability of 137Cs and 40K soil-to-fruit transfer factor in tropical lemon trees during the fruit development period

    International Nuclear Information System (INIS)

    Velasco, H.; Cid, A.S.; Anjos, R.M.; Zamboni, C.B.; Rizzotto, M.; Valladares, D.L.; Juri Ayub, J.

    2012-01-01

    In this investigation we evaluate the soil uptake of 137 Cs and 40 K by tropical plants and their consequent translocation to fruits, by calculating the soil-to-fruit transfer factors defined as F v = [concentration of radionuclide in fruit (Bq kg −1 dry mass)/concentration of radionuclide in soil (Bq kg −1 dry mass in upper 20 cm)]. In order to obtain F v values, the accumulation of these radionuclides in fruits of lemon trees (Citrus limon B.) during the fruit growth was measured. A mathematical model was calibrated from the experimental data allowing simulating the incorporation process of these radionuclides by fruits. Although the fruit incorporates a lot more potassium than cesium, both radionuclides present similar absorption patterns during the entire growth period. F v ranged from 0.54 to 1.02 for 40 K and from 0.02 to 0.06 for 137 Cs. Maximum F v values are reached at the initial time of fruit growth and decrease as the fruit develops, being lowest at the maturation period. As a result of applying the model a decreasing exponential function is derived for F v as time increases. The agreement between the theoretical approach and the experimental values is satisfactory. - Highlights: ► We assessed the transfer of 137 Cs and 40 K from soil to fruits in tropical plants. ► A mathematical model was developed to describe the dry mass growth of lemon fruits. ► The transfer factors ranged from 0.54 to 1.02 for 40 K and from 0.02 to 0.06 for 137 Cs. ► Maximum values of transfer factors were reached in the initial phase of fruit growth. ► The agreement between the theoretical and the experimental results was satisfactory.

  19. Influence of Inter stock Grafting as a Dwarfing Component on Peach Trees Development and Fruit Quality

    International Nuclear Information System (INIS)

    Hossain, S.; Nasrulhaq, A.; Mizutani, F.

    2006-01-01

    A field experiment was conducted to investigate the interstock grafting effect as a dwarfing component on Peach tree development and fruit quality. The study was made at the Ehime University Experimental farm located in south-eastern Japan during the period 2001-2005. The results of the field experiment indicated that pruned branches weight and flowers number were lower in interstock than in control trees. However, percent fruit set was a little higher in intersrock treated than in control trees. Fruit yield and fruit weight were lower in interstock treated than control trees. Soluble solids content and maturity index were higher in interstock treated trees than control, with an increasing trend in the period from 2001 to 2005, while titratable acidity showed that the livestock grafting is a useful dwarfing component for controlling the size of peach trees and improvement of fruit quality. (author)

  20. Development and quality of pineapple guava fruit in two locations with different altitudes in Cundinamarca, Colombia

    Directory of Open Access Journals (Sweden)

    Alfonso Parra-Coronado

    2015-01-01

    Full Text Available Fruit growth is stimulated by different weather conditions. The aim of this study was to determine the influence of weather conditions on the physicochemical properties of pineapple guava fruit growth. Twenty trees were marked in two production areas located at different altitudes (1,800 and 2,580 m.a.s.l., and measurements were performed every 7 days from 99 and 141 days post-anthesis to harvest at altitudes of 1,800 and 2,580 m.a.s.l., respectively. The results indicate that altitude and weather conditions greatly influence the growth and development of pineapple guava fruit, and these effects are primarily manifested in the physical characteristics of the fruit. The weight and size of the fruit at harvest are directly related to the altitude of the production area. The weather condition that has the greatest impact on total titratable acidity at harvest is cumulative radiation during fruit growth; the highest value of total soluble solids at harvest corresponds to the location with the higher altitude, lower rainfall and relative humidity and higher cumulative radiation during the fruit growth period. The hue angle and pulp firmness at harvest are not influenced by the location or weather conditions at any location and do not determine the fruit quality at harvest time.

  1. Dynamic changes in the date palm fruit proteome during development and ripening

    KAUST Repository

    Marondedze, Claudius; Gehring, Christoph A; Thomas, Ludivine

    2014-01-01

    in gel comparative proteomics combined with tandem mass spectrometry were used to study date fruit development and ripening. Total proteins were extracted using a phenol-based protocol. A total of 189 protein spots were differentially regulated (p≤0

  2. Lights and shadows of the red gold. An integrated assessment of Spanish strawberry production and its impacts.

    Science.gov (United States)

    Madrid-Lopez, Cristina

    2014-05-01

    Spanish strawberries are an export success. Spain is the second largest producer of this fruit and it has been the world's leading strawberry exporter for years. In 2012, strawberry exports were approximately 95% of total strawberry production in Spain. The production of the red gold is concentrated in around 7000 hectares of intensive farming in the province of Huelva (south west Spain). At least 85% of all Spanish strawberries are produced in this region, in an area close to the UNESCO World Heritage Site Doñana National Park. The case of the strawberry production in Huelva makes an interesting case study for the integrated analysis of the water-food-land-political economy nexus. Its combination of bio-physical and socio-economic characteristics (local climatic conditions, agricultural techniques, environmental issues, food value chains, trade networks and socio-economic factors) are quite particular and form a case where social and natural issues coevolve. Supporters of the activity argue a high contribution to local GDP and job market and its strategic position in international markets. Critics maintain that this industry creates social and environmental conflicts. The strawberry from Huelva supplies early season strawberries to the richer markets of Germany and France and at the same time seems to fail in alleviating a provincial unemployment rate of 34%. The viability of the system is in danger: production costs are 70% higher than 20 years ago while the price per kilo has not changed or even decreased. In environmental terms, the strawberries of Huelva are irrigated with ground water withdrawn from aquifers that feed the water streams of the natural park. Half of the wells that extract the water are not legally registered for what the total amount of water withdrawal remains unknown. Should we promote the continuity of this activity? In this work, I perform a Multi-Scale Integrated Assessment of Societal and Ecosystem Metabolism (MuSIASEM) of Water for the

  3. Qualitative and quantitative analysis of flavonoids of the strawberry tree - Arbutus unedo L. (Ericaceae).

    Science.gov (United States)

    Males, Zeljan; Plazibat, Misko; Vundać, Vjera Bilusić; Zuntar, Irena

    2006-06-01

    The leaves and fruits of strawberry tree - Arbutus unedo L., collected from two separate geographic locations in Croatia were investigated to determine their flavonoid composition and content. Quercitrin, isoquercitrin, hyperoside and rutin were identified in all leaf samples by means of thin-layer chromatography; the fruits contained only isoquercitrin. Chlorogenic acid was present in some leaf samples. The content of flavonoids depended on the plant organ investigated, date of collection and the locality. Spectrophotometric determination of the flavonoids indicated that the leaves are richer in flavonoids (0.52-2.00%) than fruits (0.10-0.29%).

  4. Studies on preparation of mixed toffee from guava and strawberry.

    Science.gov (United States)

    Chavan, U D; Pawar, U B; Pawar, G H

    2015-10-01

    The present investigation was carried out to develop a technology for preparation of mixed toffee from guava and strawberry pulp and to study the changes in chemical composition and sensory properties of toffee during storage at ambient temperature as well as refrigerated condition. Preliminary experiments were conducted to find out optimum levels of guava and strawberry pulp. The toffees prepared were wrapped in metallic coated polythene wrapper, packed in 200 gauge polythene bags and stored at ambient (27 ± 2 oC) as well as refrigerated (5 ± 2 oC) condition for 90 days. The stored samples were drawn periodically at 30 days interval for organoleptic and chemical analysis. Preliminary studies were carried out to standardize the optimum levels of guava and strawberry pulp. Among various combinations of guava and strawberry pulp, 70 : 30 w/w (guava : strawberry) ratios toffee was found better than other combinations in respect to organoleptic properties and nutritional quality. The yield of fresh toffee was higher (868 g/kg of pulp) in toffee prepared from 100 % guava (control). The chemical composition indicated that the fresh toffees contained on an average moisture 8.73 %, TSS 83.21 oBrix, titrable acidity 0.3 %, total sugars 73.1 % and ascorbic acid 64.1 mg/100 g. The mean score of fresh toffees for colour and appearance was 8.29, texture 8.02, flavour 8.22, taste 8.32 and overall acceptability 8.16 on 9 point Hedonic scale. The cost of fresh toffee was Rs. 282/kg which was prepared from 70 : 30 guava and strawberry pulp level. The storage studies indicated that the TSS and total sugars increased with the advancement of storage period, while moisture content, ascorbic acid and acidity decreased. The rates of increase or decrease were relatively higher at ambient temperature than refrigerated temperature. The sensory quality of toffees also decreased at faster rate during 90 days storage period at ambient condition than the refrigerated

  5. An anthocyanin-rich strawberry extract protects against oxidative stress damage and improves mitochondrial functionality in human dermal fibroblasts exposed to an oxidizing agent.

    Science.gov (United States)

    Giampieri, Francesca; Alvarez-Suarez, José M; Mazzoni, Luca; Forbes-Hernandez, Tamara Y; Gasparrini, Massimiliano; Gonzàlez-Paramàs, Ana M; Santos-Buelga, Celestino; Quiles, Josè L; Bompadre, Stefano; Mezzetti, Bruno; Battino, Maurizio

    2014-08-01

    This study investigates the protective effect of the Sveva strawberry polyphenol-rich extract on human dermal fibroblasts against AAPH-induced oxidative stress. The HPLC-DAD/ESI-MS analysis was used for evaluating the phenolic composition of the fruits. Sveva strawberry presented a high anthocyanin content (639.79 mg per kg fresh fruit), representing ∼86.08% of the total phenolic content, with Pg-3-glc as the most abundant representative (611.18 mg per kg fresh fruit). Only one ellagitannin (agrimoniin) was identified, while two quercetins, three kaempherol derivates, and three ellagic acid derivatives were detected and quantified. Strawberry pre-treatment (0.5 mg ml(-1)) markedly increased human dermal fibroblast viability, with a significant reduction of apoptotic and dead cells, and suppressed AAPH-induced ROS generation, after only 30 minutes of incubation with the oxidizing agent, and lipid peroxidation, against a range of AAPH concentrations tested. Notably, the strawberry extract also improved the mitochondrial functionality: the basal respiratory performance after treatment was ∼1.59-fold higher compared to control cells, while pre-treatment with strawberry extract before oxidative damage increased ∼2.70-fold compared to stressed cells. Our results confirm that the strawberry possesses antioxidant properties, and may be useful for the prevention of free radical-induced skin damage.

  6. Phenology and yield of strawberry as influenced by planting time and genotypes in a sub tropical region

    International Nuclear Information System (INIS)

    Rahman, M.M.; Islam, N.

    2016-01-01

    Effects of planting time on phenology of five strawberry genotypes Sweet Charlie, Festival, Camarosa, FA 008, and BARI strawberry-1 were evaluated at Bangladesh Agricultural Research Institute in two consecutive years 2009-2010 and 2010-2011. Sweet Charlie took the shorter time to begin flowering, followed by BARI Strawberry-1 and Festival when planted in 1st October. Genotype FA 008 took longer time to flower when planted in 1st December. Days to flowering of all the varieties was found to decrease with the increase in air temperature. Regardless of planting year, the genotype FA 005 followed by Camarosa and Festival, planted on 1st September, exhibited the longest harvest duration, while Sweet Charlie planted on 1st December exhibited the shortest harvest duration in both years. Genotype Festival planted on October yielded fruit with the greatest fruit weight, followed by Sweet Charlie and Camarosa planted on the same date. Plants of FA 008 and BARI Strawberry-1 planted in December produced minimum fruit weight. Maximum number of fruits/plant as well as yield/plant obtained from Sweet Charlie planted in October, while BARI Strawberry-1 planted in December yielded the least. With the use of quadratic equation it was estimated that maximum yield was obtained at ambient temperature 18.5 degree C then it was decreased with the increase of temperature. Strawberry planted in early October was found to be the most suitable in Bangladesh. Among the studied genotypes, Sweet Charlie was found to be superior in yield and early planting, and Camarosa was suitable for late planting. Festival was found less sensitive to planting date. (author)

  7. Origanum dictamnus Oil Vapour Suppresses the Development of Grey Mould in Eggplant Fruit In Vitro

    Science.gov (United States)

    Loulakakis, Kostas; Magan, Naresh; Tzortzakis, Nikos

    2014-01-01

    Grey mould rot (Botrytis cinerea) development in vitro or in eggplant (Solanum melongena L.) fruit was evaluated after treatment with dittany (Origanum dictamnus L.) oil (DIT) and storage at 12°C and 95% relative humidity during or following exposure to the volatiles. DIT volatiles used in different concentration (0-50-100-250 μL/L) and times of exposure (up to 120 h) examined the effects on pathogen development as well as fruit quality parameters. In vitro, fungal colony growth was inhibited with the application of DIT oil (during or after exposure) and/or time of application. Continuous exposure to oils reduced conidial germination and production with fungistatic effects observed in 250 μL/L. In vivo, fungal lesion growth and conidial production reduced in DIT-treated fruits. Interesting, in fruits preexposed to volatiles before fungal inoculation, DIT application induced fruit resistance against the pathogen, by reduced lesion growth and conidial production. Conidial viability reduced in >100 μL/L DIT oil. Fruits exposed to essential oil did not affect fruit quality related attributes in general, while skin lightness (L value) increased in 50 and 100 μL/L DIT oil. The results of the current study indicated that dittany volatiles may be considered as an alternative food preservative, eliminating disease spread in the storage/transit atmospheres. PMID:25254209

  8. Origanum dictamnus Oil Vapour Suppresses the Development of Grey Mould in Eggplant Fruit In Vitro

    Directory of Open Access Journals (Sweden)

    Andriana Stavropoulou

    2014-01-01

    Full Text Available Grey mould rot (Botrytis cinerea development in vitro or in eggplant (Solanum melongena L. fruit was evaluated after treatment with dittany (Origanum dictamnus L. oil (DIT and storage at 12°C and 95% relative humidity during or following exposure to the volatiles. DIT volatiles used in different concentration (0-50-100-250 μL/L and times of exposure (up to 120 h examined the effects on pathogen development as well as fruit quality parameters. In vitro, fungal colony growth was inhibited with the application of DIT oil (during or after exposure and/or time of application. Continuous exposure to oils reduced conidial germination and production with fungistatic effects observed in 250 μL/L. In vivo, fungal lesion growth and conidial production reduced in DIT-treated fruits. Interesting, in fruits preexposed to volatiles before fungal inoculation, DIT application induced fruit resistance against the pathogen, by reduced lesion growth and conidial production. Conidial viability reduced in >100 μL/L DIT oil. Fruits exposed to essential oil did not affect fruit quality related attributes in general, while skin lightness (L value increased in 50 and 100 μL/L DIT oil. The results of the current study indicated that dittany volatiles may be considered as an alternative food preservative, eliminating disease spread in the storage/transit atmospheres.

  9. Fruit development, pigmentation and biochemical properties of wax apple as affected by localized Application of GA3 under field conditions

    OpenAIRE

    Khandaker, Mohammad Moneruzzaman; Boyce, Amru Nasrulhaq; Osman, Normaniza; Golam, Faruq; Rahman, M. Motior; Sofian-Azirun, M.

    2013-01-01

    This study investigated the effects of gibberellin (GA3) on the fruit development, pigmentation and biochemical properties of wax apple. The wax apple trees were rubbing treated with 0, 20, 50 and 100 mgGA3/l under field conditions. The localized application (rubbing) of 50 mg GA3/l significantly increased the fruit set, fruit length and diameter, color development, weight and yieldcompared to the control. In addition, GA3 treatments significantly reduced the fruit drop. With regard to the fr...

  10. Dynamic changes in the date palm fruit proteome during development and ripening

    KAUST Repository

    Marondedze, Claudius

    2014-08-06

    Date palm (Phoenix dactylifera) is an economically important fruit tree in the Middle East and North Africa and is characterized by large cultivar diversity, making it a good model for studies on fruit development and other important traits. Here in gel comparative proteomics combined with tandem mass spectrometry were used to study date fruit development and ripening. Total proteins were extracted using a phenol-based protocol. A total of 189 protein spots were differentially regulated (p≤0.05). The identified proteins were classified into 14 functional categories. The categories with the most proteins were ‘disease and defense’ (16.5%) and ‘metabolism’ (15.4%). Twenty-nine proteins have not previously been identified in other fleshy fruits and 64 showed contrasting expression patterns in other fruits. Abundance of most proteins with a role in abiotic stress responses increased during ripening with the exception of heat shock proteins. Proteins with a role in anthocyanin biosynthesis, glycolysis, tricarboxylic acid cycle and cell wall degradation were upregulated particularly from the onset of ripening and during ripening. In contrast, expression of pentose phosphate- and photosynthesis-related proteins decreased during fruit maturation. Although date palm is considered a climacteric species, the analysis revealed downregulation of two enzymes involved in ethylene biosynthesis, suggesting an ethylene-independent ripening of ‘Barhi’ fruits. In summary, this proteomics study provides insights into physiological processes during date fruit development and ripening at the systems level and offers a reference proteome for the study of regulatory mechanisms that can inform molecular and biotechnological approaches to further improvements of horticultural traits including fruit quality and yield.

  11. Correlation between Strawberry (Fragaria ananassa Duch. Productivity and Photosynthesis-related Parameters under Various Growth Conditions

    Directory of Open Access Journals (Sweden)

    Hyo Gil Choi

    2016-10-01

    Full Text Available In the present study, we investigated changes in chlorophyll fluorescence, photosynthetic parameters and fruit yields, as well as fruit phytochemical accumulation of strawberry (Fragaria ananassa Duch. that had been cultivated in a greenhouse under different combinations of light intensity and temperature. In plants grown with low light (LL photosystem II chlorophyll fluorescence was found to increase as compared with those grown under high light (HL. When strawberry plants were grown with temperature higher than 5◦C in addition to LL, they showed decrease in non-photochemical quenching (NPQ, photochemical quenching (qP, as well as chlorophyll fluorescence decrease ratio (RFd when compared with other combinations of light and temperature. Moreover, fruit yield of strawberry was closely correlated with chlorophyll fluorescence-related parameters such as NPQ, qP, and RFd, but not with the maximum efficiency of PS II (Fv/Fm. Although plant groups grown under different combinations of light and temperature showed almost comparable levels of photosynthesis rates (Pr when irradiated with low-intensity light, they displayed clear differences when measured with higher irradiances. Plants grown under HL with temperature above 10◦C showed the highest Pr, in contrast to the plants grown under LL with temperature above 5◦C. When the stomatal conductance and the transpiration rate were measured, plants of each treatment showed clear differences even when analyzed with lower irradiances. We also found that fruit production during winter season was more strongly influenced by growth temperature than light intensity. We suggest that fruit productivity of strawberry is closely associated with chlorophyll fluorescence and photosynthesis-related parameters during cultivation under different regimes of temperature and light.

  12. Development of Bait Stations for Fruit Fly Suppression in Support of SIT. Working Material

    International Nuclear Information System (INIS)

    2009-01-01

    A more economic and practical fruit fly suppression tool is needed to replace conventional aerial and ground bait sprays applications over human settlements, protected natural areas, and difficult to access areas where fruit fly hosts exist. This has been a major request from area-wide integrated pest management action programmes using the Sterile Insect Technique (SIT) as a component. In recent years, especially in Europe, most conventional insecticides used to control fruit pests have been banned (e.g. malathion, dichlorvos and other organophosphates), therefore areas producing fruits and vegetables for markets that request low insecticide residues or even fruit and vegetable organic farming is seeking for a more economic fruit fly control option to the spinosad-based bait sprays and to the use of mass trapping. To address these requests, bait stations can be one of the most suitable alternatives. The development of these devices needs to take into consideration cost-effectiveness, and long lasting attractants and killing agents, and should target female fruit flies. Recent developments of synthetic food attractants and long-lasting formulations open the possibility to improve the existent baits stations or develop new ones. With this objective the Insect Pest Control Subprogramme of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture organized a Consultants Meeting ('Development of Bait Stations for Fruit Fly Suppression in Support of SIT'), held in Mazatlan, Mexico, from 30 October to 1 November 2008, with the participation of 14 scientists from the Instituto Nacional de Tecnologia Agropecuaria, Argentina; Department of Primary Industries and Fisheries, Australia; North American Plant Protection Organization, Canada; African Insect Science for Food and Health, Kenya; Universidad Politecnica de Valencia, Spain; Institut de Recerca i Tecnologia Agroalimentaries, Spain, Secretaria de Agricultura, Ganaderia, Desarrollo Rural, Pesca y

  13. Development of molecular tests for the detection of ILAR and latent viruses in fruit trees.

    Science.gov (United States)

    Roussel, S; Kummert, J; Dutrecq, O; Lepoivre, P; Jijakli, M H

    2004-01-01

    The detection throughout the year of latent and ILAR viruses in fruit tress by classical serological tests appear to be unreliable. We have developed RT-PCR tests for a reliable detection of latent and ILAR viruses in fruit trees. These assays were then simplified to allow the direct use of crude plant extracts instead of total RNA preparations, and the analyses of pooled samples. In this way, such RT-PCR protocols are suitable for a routine diagnosis of latent and ILAR viruses in fruit tree certification.

  14. Molecular Diversity of Anthracnose Pathogen Populations Associated with UK Strawberry Production Suggests Multiple Introductions of Three Different Colletotrichum Species.

    Directory of Open Access Journals (Sweden)

    Riccardo Baroncelli

    Full Text Available Fragaria × ananassa (common name: strawberry is a globally cultivated hybrid species belonging to Rosaceae family. Colletotrichum acutatum sensu lato (s.l. is considered to be the second most economically important pathogen worldwide affecting strawberries. A collection of 148 Colletotrichum spp. isolates including 67 C. acutatum s.l. isolates associated with the phytosanitary history of UK strawberry production were used to characterize multi-locus genetic variation of this pathogen in the UK, relative to additional reference isolates that represent a worldwide sampling of the diversity of the fungus. The evidence indicates that three different species C. nymphaeae, C. godetiae and C. fioriniae are associated with strawberry production in the UK, which correspond to previously designated genetic groups A2, A4 and A3, respectively. Among these species, 12 distinct haplotypes were identified suggesting multiple introductions into the country. A subset of isolates was also used to compare aggressiveness in causing disease on strawberry plants and fruits. Isolates belonging to C. nymphaeae, C. godetiae and C. fioriniae representative of the UK anthracnose pathogen populations showed variation in their aggressiveness. Among the three species, C. nymphaeae and C. fioriniae appeared to be more aggressive compared to C. godetiae. This study highlights the genetic and pathogenic heterogeneity of the C. acutatum s.l. populations introduced into the UK linked to strawberry production.

  15. Molecular Diversity of Anthracnose Pathogen Populations Associated with UK Strawberry Production Suggests Multiple Introductions of Three Different Colletotrichum Species

    Science.gov (United States)

    Baroncelli, Riccardo; Zapparata, Antonio; Sarrocco, Sabrina; Sukno, Serenella A.; Lane, Charles R.; Thon, Michael R.; Vannacci, Giovanni; Holub, Eric; Sreenivasaprasad, Surapareddy

    2015-01-01

    Fragaria × ananassa (common name: strawberry) is a globally cultivated hybrid species belonging to Rosaceae family. Colletotrichum acutatum sensu lato (s.l.) is considered to be the second most economically important pathogen worldwide affecting strawberries. A collection of 148 Colletotrichum spp. isolates including 67 C. acutatum s.l. isolates associated with the phytosanitary history of UK strawberry production were used to characterize multi-locus genetic variation of this pathogen in the UK, relative to additional reference isolates that represent a worldwide sampling of the diversity of the fungus. The evidence indicates that three different species C. nymphaeae, C. godetiae and C. fioriniae are associated with strawberry production in the UK, which correspond to previously designated genetic groups A2, A4 and A3, respectively. Among these species, 12 distinct haplotypes were identified suggesting multiple introductions into the country. A subset of isolates was also used to compare aggressiveness in causing disease on strawberry plants and fruits. Isolates belonging to C. nymphaeae, C. godetiae and C. fioriniae representative of the UK anthracnose pathogen populations showed variation in their aggressiveness. Among the three species, C. nymphaeae and C. fioriniae appeared to be more aggressive compared to C. godetiae. This study highlights the genetic and pathogenic heterogeneity of the C. acutatum s.l. populations introduced into the UK linked to strawberry production. PMID:26086351

  16. Frozen fruit skin prick test for the diagnosis of fruit allergy.

    Science.gov (United States)

    Garriga, Teresa; Guilarte, Mar; Luengo, Olga; Guillén, Mercé; Labrador-Horrillo, Moisés; Fadeeva, Tatiana; Sala, Anna; Cardona, Victória

    2010-12-01

    Diagnosis of fruit sensitisation by skin prick test (SPT) is fast and easy to perform. Nevertheless, some fruit is not available throughout the year. Freezing aliquots of these fresh fruits to be defrosted would be a good solution to perform SPT at any time. To compare the reproducibility of SPT with Rosaceae and Cucurbitaceae frozen fruit with fresh and commercial fruit extracts. SPT with the following fruit were performed: apricot, cherry, strawberry, nectarine, Japanese medlar, peach, (peel and pulp), yellow and red plum, melon and watermelon. We compared fresh fruit, commercial extract and fruit which had been frozen at -18 degrees C. Results were read by planimetry (Inmunotek prick-film) after 15 minutes. The study group comprised 48 patients (9 males, 39 females) with a mean age of 31, 6 +/- 2.0 years. Concordance of positive and negative results was extremely high and significant in all cases. Correlation between frozen fruit and commercial extract, frozen fruit and fresh and commercial extract and fresh fruit was statistically significant in all cases except for strawberry. The use of frozen fruit is a valid method, as the performance of the SPT is similar to that of fresh fruit. This enables diagnostic procedures with seasonal fruit at any time of the year.

  17. Effects of Polysaccharide-Based Edible Coatings on Quality and Antioxidant Enzyme System of Strawberry during Cold Storage

    Directory of Open Access Journals (Sweden)

    Li Li

    2017-01-01

    Full Text Available Strawberry is a nutritious, but highly perishable fruit. Three polysaccharide-based edible coatings (alginate, chitosan, and pullulan were applied to postharvest strawberry fruit during cold storage (4°C, and their effects on fruit quality and antioxidant enzyme system were investigated in the present study. The results showed that polysaccharide coatings showed a significant delay in fruit softening and rot and reduced changes in total soluble solid and titratable acidity content during 16 d storage. Polysaccharide coatings also maintained higher ascorbic acid and total phenolic contents than control from day 2 and significantly inhibited fruit decay and respiration after 12 d storage (p<0.05. Polysaccharide treatments enhanced the activities of antioxidant enzymes (peroxidase, catalase, superoxide dismutase, and ascorbate peroxidase so as to prevent lipid peroxidation and reduce membrane damage. Additionally, chitosan coating had the most positive effects on fruit quality amongst three polysaccharide-based edible coatings and presented the highest relative activities of antioxidant enzymes. These results indicated that polysaccharide-based edible coatings were helpful in postharvest quality maintenance of strawberry fruit.

  18. The effects of gamma-ray irradiation to strawberry (Fragaria x ananassa Duch.) calli on shoot regeneration, achene formation and morphological variations of regenerants

    International Nuclear Information System (INIS)

    Kasumi, Masakazu

    2002-01-01

    Strawberry (Fragaria x ananassa 'Nyoho') calli, derived from anthers, were irradiated with gamma -ray at dosages from 100 to 1,600 Gy (10 Gy · hr - 1) and their effects on callus growth, plant regeneration, and somaclonal variation were analyzed. The growth of callus and percentage of plant regeneration from the callus were reduced slightly at dosages less than 200 Gy, moderately at 400 Gy, and markedly above 800 Gy. Fruit growth associated with seed fertility (>50 % ) was morphologically normal at less than 100 Gy, whereas achene formation was significantly inhibited above 200 Gy. The percentages of morphological variation in regenerants formed from the irradiated callus were:13.7 (no irradiation), 18.6 (100 Gy), 66.0 (200 Gy), 75.7 (400 Gy), and 97.0 (800 Gy). Furthermore, higher dosages yielded wider variations, e.g., thick and small leaf, light leaf color, white flesh, and long fruit. In some regenerants, more than one morphological aberrations developed, some of which were transmissible to daughter plants. These results revealed that the irradiating strawberry callus with gamma - ray resulted in a higher degree and wider spectrum of somaclonal variation than did simple callus culture. (author)

  19. Genomics of pear and other Rosaceae fruit trees.

    Science.gov (United States)

    Yamamoto, Toshiya; Terakami, Shingo

    2016-01-01

    The family Rosaceae includes many economically important fruit trees, such as pear, apple, peach, cherry, quince, apricot, plum, raspberry, and loquat. Over the past few years, whole-genome sequences have been released for Chinese pear, European pear, apple, peach, Japanese apricot, and strawberry. These sequences help us to conduct functional and comparative genomics studies and to develop new cultivars with desirable traits by marker-assisted selection in breeding programs. These genomics resources also allow identification of evolutionary relationships in Rosaceae, development of genome-wide SNP and SSR markers, and construction of reference genetic linkage maps, which are available through the Genome Database for the Rosaceae website. Here, we review the recent advances in genomics studies and their practical applications for Rosaceae fruit trees, particularly pear, apple, peach, and cherry.

  20. Effect of different planting time on different varieties of strawberries

    Science.gov (United States)

    Zhong, Yue; Luo, Ya; Ge, Cong; Mo, Qin; Lin, Yajie; Luo, Shu; Tang, Haoru

    2018-04-01

    The experiment chose two strawberry varieties which planted in two periods of September 10 and September 20, in order to identify the optimum planting time of strawberries by exploring the effects of different planting time on strawberry quality and flowering initially. The results showed that different planting time will affect the growth and quality of strawberry, and the quality of September 10 planting strawberries is better than September 20 planting strawberries while there do exist some differences between strawberry varieties. In summary, the preliminary determination is that in Hanyuan area, the Hong Yan and Zhang Ji's optimum planting time is 10 September.

  1. Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells.

    Science.gov (United States)

    Giampieri, Francesca; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y; Mazzoni, Luca; Capocasa, Franco; Sabbadini, Silvia; Alvarez-Suarez, Josè M; Afrin, Sadia; Rosati, Carlo; Pandolfini, Tiziana; Molesini, Barbara; Sánchez-Sevilla, José F; Amaya, Iraida; Mezzetti, Bruno; Battino, Maurizio

    2018-01-24

    Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

  2. Ziziphus mauritiana (masau) fruits fermentation in Zimbabwe: from black-box to starter culture development

    OpenAIRE

    Nyanga, L.K.

    2012-01-01

    This thesis reports on studies of microbiological and biochemical properties of masau (Ziziphus mauritiana) fruit fermentation and the development of starter cultures for the production of masau beverages. A survey to document the traditional processing techniques was conducted using a questionnaire and focus group discussions in each of the three districts, i.e., Mudzi, Mt Darwin and Muzarabani in Zimbabwe. The survey results showed that the masau fruit is usually gathered by women and chi...

  3. Thermal requirements and estimate of the annual number of generations of Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) on strawberry crop; Exigencias termicas e estimativa do numero de geracoes anuais de Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) em morangueiro

    Energy Technology Data Exchange (ETDEWEB)

    Nondillo, Aline; Redaelli, Luiza R.; Pinent, Silvia M.J.; Gitz, Rogerio [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Programa de Pos-Graduacao em Fitotecnica. Dept. de Fitossanidade]. E-mails: RS; alinondillo@yahoo.com.br, luredael@ufrgs.br; silviapi@portoweb.com.br; rogitz29@yahoo.com.br; Botton, Marcos [Embrapa Uva e Vinho, Bento Goncalves, RS (Brazil)]. E-mail: marcos@cnpuv.embrapa.br

    2008-11-15

    Frankliniella occidentalis (Pergande) is one of the major strawberry pests in southern Brazil. The insect causes russeting and wither in flowers and fruits reducing commercial value. In this work, the thermal requirements of the eggs, larvae and pupae of F. occidentalis were estimated. Thrips development was studied in folioles of strawberry plants at six constant temperatures (16, 19, 22, 25, 28 and 31 deg C) in controlled conditions (70 +- 10% R.H. and 12:12 L:D). The number of annual generations of F. occidentalis was estimated for six strawberry production regions of Rio Grande do Sul State based on its thermal requirements. Developmental time of each F. occidentalis stages was proportional to the temperature increase. The best development rate was obtained when insects were reared at 25 deg C and 28 deg C. The lower threshold and the thermal requirements for the egg to adult stage were 9.9 deg C and 211.9 degree-days, respectively. Considering the thermal requirements of F. occidentalis, 10.7, 12.6, 13.1, 13.6, 16.5 and 17.9 generations/year were estimated, respectively, for Vacaria, Caxias do Sul, Farroupilha, Pelotas, Porto Alegre and Taquari producing regions located in Rio Grande do Sul State, Brazil. (author)

  4. Radiation preservation of foods of plant origin. Part V. Temperate fruits: pome fruits, stone fruits, and berries

    International Nuclear Information System (INIS)

    Thomas, P.

    1986-01-01

    The current status of research on the application of ionizing radiation for improving the storage of temperate fruits, i.e., apple, pear, peach, nectarine, apricot, cherry, plum, strawberry, bilberry, cranberry, raspberry, and black currant, is reviewed. Changes in fruit metabolism, chemical composition, texture, and organoleptic quality attributes are discussed with reference to the irradiation dose. The feasibility of using radiation either alone or in conjunction with heat treatment, refrigeration, and controlled atmospheres (CA) for the control of storage decay caused by fungal pathogens is considered. Areas of further research are suggested before irradiation could be considered for practical application in some of these temperate fruits. The recent trends in the possible use of irradiation for disinfestation of certain pome and stone fruits and the prospects for the commercial utilization of irradiation for improving the market life of strawberries are discussed. 156 references

  5. The stability of dichlofluanid and vinclozolin and their influence on the quality of strawberries.

    Science.gov (United States)

    Davídek, J; Hajslová, J; Svobodová, Z

    1981-05-01

    The content of dichlofluanid and vinclozolin found on strawberries treated with Euoparen and/or Ronilan, respectively, did not exceed the residue tolerance. Further decrease of the amount of these fungicides occurred during heatsterilisation of the fruit and storage of the products. The rate of decomposition of dichlofluanid and/or vincolozolin in model solutions (pH 3.0-6.0) followed pseudo-first-order kinetics. The taste and flavour of untreated fruit was preferred to those of strawberries treated with fungicides. There were differences in the content of reducing sugars, volatile fatty acids and titrable acidity in individual samples. Gas-chromatographic profiles of volatile substances isolated from treated and untreated berries also differed.

  6. Extending shelf-life and economic evaluation of strawberry irradiated in Egypt

    International Nuclear Information System (INIS)

    Mahmoud, A.A.

    1988-01-01

    This study was made on ''Egypt's mega gamma I'' irradiator the radiation which is cobalt-60. The following dose was studied in detail 3.5 KGy, was compared with unirradiated sample was studied at the same time. The storage conditions for refrigeration were 4+-1 0 C. During storage periodic fruits were carried out as certain organoleptic properties. The refrigerated irradiated fruits have a shelf life of more than 30 days, whereas the unirradiated ones last only up to 14 days. On the other side the study of economic evaluation of irradiated strawberry showed that the activity of cobalt-60 source needed to satisfy requirement of gamma-irradiation processing of the annual strawberry production in Egypt has been figured a 0.563 Mci for a total activity

  7. Cassava starch edible coating incorporated with propolis on bioactive compounds in strawberries

    Directory of Open Access Journals (Sweden)

    Ariela Betsy Thomas

    2016-02-01

    Full Text Available ABSTRACT Strawberry is a fruit appreciated throughout the world due to its attractive quality attributes and stands out due to its high phenolic compound content, which positively contribute to biological properties of nutritional interest. The objective of this study was to evaluate the effect of cassava starch coatings incorporated with propolis combinations on the phytochemical content and the maintenance and increase of the strawberry antioxidant activity. The treatments were 3% cassava starch (CS, 3% cassava starch + 33% ethanolic propolis extract (CS + P33%, 3% cassava starch + 66% ethanolic propolis extract (CS + P66% and control (C. The fruits were stored at 4 °C ± 0.5 ºC and 90%RH for 16 days, making up a completely randomized design with 4 treatments and 5 time evaluations. Vitamin C, phenolic compound, anthocyanin, and antioxidant activity levels were evaluated through two methods. The coating with 66% of propolis promoted higher Vitamin C content than fruits submitted to the other treatments at 8 and 12 days of storage. For antioxidant activity, fruits treated with CS maintained a higher FRS percentage (free radical scavenging at all time evaluations. Control fruits presented higher anthocyanin content at the last evaluation time when the highest antioxidant capacity, by the ABTS method (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid, was observed in fruits with CS and CS + P66% treatments. There was an increase tendency of the phenolic content during storage in all evaluated fruits. The propolis concentrations used, however, were not sufficient to increase or maintain the antioxidant capacity and phenolic contents of strawberries.

  8. Solidago canadensis L. Essential Oil Vapor Effectively Inhibits Botrytis cinerea Growth and Preserves Postharvest Quality of Strawberry as a Food Model System.

    Science.gov (United States)

    Liu, Shumin; Shao, Xingfeng; Wei, Yanzhen; Li, Yonghua; Xu, Feng; Wang, Hongfei

    2016-01-01

    This study investigated the anti-fungal properties of Solidago canadensis L. essential oil (SCLEO) against Botrytis cinerea in vitro, and its ability to control gray mold and maintain quality in strawberry fruits. SCLEO exhibited dose-dependent antifungal activity against B. cinerea and profoundly altered mycelial morphology, cellular ultrastructure, and membrane permeability as evaluated by scanning electron microscopy, transmission electron microscopy, and fluorescence microscopy. SCLEO vapor at 0.1 mL/L maintained higher sensory acceptance and reduced decay of fresh strawberry fruit, and also reduced gray mold in artificially inoculated fruit. SCLEO treatment did not, however, stimulate phenylalanin ammonia-lyase, polyphenol oxidase, or chitinase, enzymes related to disease resistance. This suggests that SCLEO reduces gray mold by direct inhibition of pathogen growth. SCLEO vapor may provide a new and effective strategy for controlling postharvest disease and maintaining quality in strawberries.

  9. Biosynthesis of 4-hydroxy-2,5-dimethyl-3(2H)-furanone and derivatives in in vitro grown strawberries.

    Science.gov (United States)

    Pérez, A G; Olías, R; Olías, J M; Sanz, C

    1999-02-01

    The biosynthesis of 2,5-dimethyl-4-hydroxy-3(2H)-furanone (Furaneol) and its methyl ether and glucoside derivatives has been studied in strawberries. An in vitro system was used for growing this fruit, showing that the presence in the incubation medium of sucrose or hydroxyquinoline hemisulfate has no effect on the bioformation of these compounds. Strawberries in vitro grown showed an increase in furanone content with time, especially between the second and fourth days, to the same extent as field-grown fruits but at a higher rate. Among the precursors added to the incubation medium, D-fructose gave rise to an increase in furaneol and its glucoside derivative of 42. 6% and 26.3%, respectively. D-fructose 6-phosphate seems to be the precursor of furaneol in strawberries since, when present in the incubation medium, it produced an average increase of 125% in all furanones contents with respect to control fruits.

  10. Solidago canadensis L essential oil vapor effectively inhibits Botrytis cinerea growth and preserves postharvest quality of strawberry as a food model system

    Directory of Open Access Journals (Sweden)

    Shumin Liu

    2016-08-01

    Full Text Available This study investigated the anti-fungal properties of Solidago canadensis L essential oil (SCLEO against Botrytis cinerea in vitro, and its ability to control gray mold and maintain quality in strawberry fruits. SCLEO exhibited dose-dependent antifungal activity against B. cinerea and profoundly altered mycelial morphology, cellular ultrastructure, and membrane permeability as evaluated by scanning electron microscopy, transmission electron microscopy, and fluorescence microscopy. SCLEO vapor at 0.1 mL/L maintained higher sensory acceptance and reduced decay of fresh strawberry fruit, and also reduced gray mold in artificially inoculated fruit. SCLEO treatment did not however, stimulate phenylalanin ammonia-lyase (PAL, polyphenol oxidase (POD, or chitinase (CHI, enzymes related to disease resistance. This suggests that SCLEO reduces gray mold by direct inhibition of pathogen growth. SCLEO vapor may provide a new and effective strategy for controlling postharvest disease and maintaining quality in strawberries.

  11. The development of tomatillo (Physalis ixocarpa Brot. in Polish conditions. II. Flowering and fruiting

    Directory of Open Access Journals (Sweden)

    Leszek S. Jankiewicz

    2013-12-01

    Full Text Available The reproductive development of tomatillo (husk tomato was investigated in the conditions of central Poland. The developmental cycle of tomatillo in Poland lasts 20-23 weeks, including 6 weeks in a greenhouse or a tunnel and is longer than in Mexico (15 weeks. The plant grows well in Poland and is fruiting aboundantly. The cv. Rendidora B1 was early fruiting and sensitive to drought so it should be cultivated in a garden. Cvs Bujna and Antocyjanowa were medium late or late and suitable for open-field cultivation. Fruit development lasts about 6 weeks. Fruiting was concentrated on the apparent lateral branches of the 1st and 2nd order during the large part of a vegetative season. Among the abscised generative organs predominated flowers and at the end of vegetative season the flower buds. The maximum abscission of flower buds and flowers took place about 2-4 weeks after the most intensive flowering and fruit set. The fruits of many individuals are easily cracking. After being abscised they are attacked frequently by Botrytis sp.

  12. Effect of planting density and growing media on growth and yield of strawberry

    International Nuclear Information System (INIS)

    Tariq, R.; Qureshi, K.M.; Hassan, I.; Rasheed, M.; Qureshi, U.S.

    2013-01-01

    Strawberry (Fragaria ananasa), belonging to Rosaceae family, is a rich source of vitamins and minerals with delicate flavors. It is perishable crop which is exceedingly in demand for its taste, profitability, high yield and good quality. To make the plant growth successful in the container, the requirement of special media is very important step because plant growth is largely depended on the physiochemical properties of the growing media used. Winter strawberry production in a greenhouse using high plant densities and various media may be a viable alternative to open-field production system. Planting density can be increased thrice by using different production systems. Studies were conducted to see the impact of different planting densities and media on growth and yield of strawberry. The treatments were T 1 = Control, with normal planting distance of 30 cm x 60 cm and growing media silt, sand and farm yard manure (FYM); T 2 = 15 cm 2 x 30 cm and silt, sand and FYM; T 3 = 30 cm x 60 cm and coir; T 4 = 15 cm x 30 cm and coir; T 5 = 30 cm x 60 cm and peat moss; T 6 = 15 cm x 30 cm and 5 6 peat moss. Results showed that plants grown at low planting distance on all growth media showed more pronounced results as compared to high planting distance. Plants grown in peat moss at both planting densities moderately increased the plant height, canopy size, leaf area, number of fruits, fruit size, fruit weight and titratable acidity. A significant increase in fresh and dry weight of leaves, number of leaves, fruit yield in term of fruit number, fruit size and fruit weight, and fruit quality with high ascorbic acid contents were observed. On the other hand, plants grown in silt, sand and FYM (1 : 1 : 1) at both planting densities showed significant increment in vegetative growth resulting in early flowering with more flowers per plant, better fruit setting and fruit set percentage, greater fruit size and weight but fruit number per plant was reduced which lowered the overall

  13. Characterization of transcriptome dynamics during watermelon fruit development: sequencing, assembly, annotation and gene expression profiles.

    Science.gov (United States)

    Guo, Shaogui; Liu, Jingan; Zheng, Yi; Huang, Mingyun; Zhang, Haiying; Gong, Guoyi; He, Hongju; Ren, Yi; Zhong, Silin; Fei, Zhangjun; Xu, Yong

    2011-09-21

    Cultivated watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus] is an important agriculture crop world-wide. The fruit of watermelon undergoes distinct stages of development with dramatic changes in its size, color, sweetness, texture and aroma. In order to better understand the genetic and molecular basis of these changes and significantly expand the watermelon transcript catalog, we have selected four critical stages of watermelon fruit development and used Roche/454 next-generation sequencing technology to generate a large expressed sequence tag (EST) dataset and a comprehensive transcriptome profile for watermelon fruit flesh tissues. We performed half Roche/454 GS-FLX run for each of the four watermelon fruit developmental stages (immature white, white-pink flesh, red flesh and over-ripe) and obtained 577,023 high quality ESTs with an average length of 302.8 bp. De novo assembly of these ESTs together with 11,786 watermelon ESTs collected from GenBank produced 75,068 unigenes with a total length of approximately 31.8 Mb. Overall 54.9% of the unigenes showed significant similarities to known sequences in GenBank non-redundant (nr) protein database and around two-thirds of them matched proteins of cucumber, the most closely-related species with a sequenced genome. The unigenes were further assigned with gene ontology (GO) terms and mapped to biochemical pathways. More than 5,000 SSRs were identified from the EST collection. Furthermore we carried out digital gene expression analysis of these ESTs and identified 3,023 genes that were differentially expressed during watermelon fruit development and ripening, which provided novel insights into watermelon fruit biology and a comprehensive resource of candidate genes for future functional analysis. We then generated profiles of several interesting metabolites that are important to fruit quality including pigmentation and sweetness. Integrative analysis of metabolite and digital gene expression

  14. Effects of gamma radiation and packaging on conservation of toyonoka strawberries stored under refrigeration

    International Nuclear Information System (INIS)

    Domingues, Danielle Marinelli

    2000-01-01

    Strawberries have short post harvest life due to the rapid spoilage caused by fungal infections, even when stored under refrigeration. The effects of gamma radiation (0.0, 1.5, 2.0 and 2.5 kGy) and two kinds of packages (plastic boxes with or without PVC film wrapping) were evaluated using Toyonoka strawberries kept under refrigeration (3 to 6 deg C and relative humidity of 55%) for 1, 8, 15 and 22 days after irradiation, applied 1 day after harvesting. Deterioration progression and loss of weight occurred in the course of storage and although the content of ascorbic acid had also increased, total titratable acidity, p H and ratio remained constant during all the experimental period, independent of the samples treatments. The use of PVC film packing inhibited dehydration of the strawberries along the storage and also caused reduction in soluble solid content of the fruits stored for more than 8 days. The treatments with 2.0 and 2.5 kGy had caused significant losses of ascorbic acid the higher dose also significantly reduced the texture of the fruits. Sensorial analysis has been performed with 30 untrained judges to evaluate the acceptability of strawberries packed without the PVC wrapping and irradiated with doses of 0.0, 1.5 or 2.0 kGy. No irradiation effects were observed and the samples were acceptable with no significant difference between samples stored for 1 or 8 days after irradiation. PVC film packaging and irradiation were not efficient in delaying post harvest decay of Toyonoka strawberries in the conditions used in these experiments. (author)

  15. Conservation by irradiation of the cooled strawberries

    International Nuclear Information System (INIS)

    Kaabi, Olfa

    2004-01-01

    During this work, the microbiological, physicochemical and organoleptic effects of the irradiation were studied throughout the strawberries conservation at 4 0 C in camarosa variety. Thus, the optimal dose was measured. The experimental results showed that the ionizing treatment causes a notable reduction of the microbial flora (total yeasts and moulds, psychrotrophes germs) of the strawberry. This reduction can exceed the 90% of the total flora in the strawberries treated with 2.5 and 3 KGy. The microbial load remained no significant for the strawberries treated with 2, 2.5 and 3 KGy until the 21 2nd day of the conservation. On the organoleptic level, the gustatory quality of the strawberries irradiated by 2 and 2.5 KGy is acceptable, whereas the control strawberries exposed at 3 KGy are acceptable. Moreover, the effect of the irradiation on the physico-chemical parameters is significant only in the case of the ascorbic acid which notes a considerable reduction in the vitamin during the first days of conservation, but which will be moderated after 8 2nd days of the storage cutters irradiated. This degradation remained more accelerated in the case of control strawberries. Lastly, we can affirm that the irradiation increases the shelf life without making modifications sensitive to the original characteristics of the cutter, which constitutes a reliable process and inexpensive in energy. (author). 13 refs

  16. Investigation of fruit irradiation: bibliographical review

    International Nuclear Information System (INIS)

    Luna C, P.C.

    1991-01-01

    It was carried out a bibliographical review that embraces the years 1984-1987, on the relating works to the irradiation of some fruits like the apple, date, peach, plum, cherry, papaya, grape, banana, pear and strawberry. The purpose is to have a reference on the doses and the conditions used by several investigators for some fruits, as for its disinfestation and extension of shelf life. (Author)

  17. Genetic regulation and structural changes during tomato fruit development and ripening

    Directory of Open Access Journals (Sweden)

    Paolo ePesaresi

    2014-04-01

    Full Text Available Fruits are an important evolutionary acquisition of angiosperms, which afford protection for seeds and ensure their optimal dispersal in the environment. Fruits can be divided into dry or fleshy. Dry fruits are the more ancient and provide for mechanical seed dispersal. In contrast, fleshy fruits develop soft tissues in which flavour compounds and pigments accumulate during the ripening process. These serve to attract animals that eat them and disseminate the indigestible seeds. Fruit maturation is accompanied by several striking cytological modifications. In particular, plastids undergo significant structural alterations, including the dedifferentiation of chloroplasts into chromoplasts. Chloroplast biogenesis, their remodeling in response to environmental constraints and their conversion into alternative plastid types are known to require communication between plastids and the nucleus in order to coordinate the expression of their respective genomes. In this review, we discuss the role of plastid modifications in the context of fruit maturation and ripening, and consider the possible involvement of organelle-nucleus crosstalk via retrograde (plastid to nucleus and anterograde (nucleus to plastid signaling in the process.

  18. The role of abscisic acid in regulating cucumber fruit development and ripening and its transcriptional regulation.

    Science.gov (United States)

    Wang, Yanping; Wang, Ya; Ji, Kai; Dai, Shengjie; Hu, Ying; Sun, Liang; Li, Qian; Chen, Pei; Sun, Yufei; Duan, Chaorui; Wu, Yan; Luo, Hao; Zhang, Dian; Guo, Yangdong; Leng, Ping

    2013-03-01

    Cucumber (Cucumis sativus L.), a kind of fruit usually harvested at the immature green stage, belongs to non-climacteric fruit. To investigate the contribution of abscisic acid (ABA) to cucumber fruit development and ripening, variation in ABA level was investigated and a peak in ABA level was found in pulp before fruit get fully ripe. To clarify this point further, exogenous ABA was applied to cucumber fruits at two different development stages. Results showed that ABA application at the turning stage promotes cucumber fruit ripening, while application at the immature green stage had inconspicuous effects. In addition, with the purpose of understanding the transcriptional regulation of ABA, two partial cDNAs of CsNCED1 and CsNCED2 encoding 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in ABA biosynthetic pathway; one partial cDNA of CsCYP707A1 for 8'-hydroxylase, a key enzyme in the oxidative catabolism of ABA and two partial cDNAs of CsBG1 and CsBG2 for β-glucosidase (BG) that hydrolyzes ABA glucose ester (ABA-GE) to release active ABA were cloned from cucumber. The DNA and deduced amino acid sequences of these obtained genes respectively showed high similarities to their homologous genes in other plants. Real-time PCR analysis revealed that ABA content may be regulated by its biosynthesis (CsNCEDs), catabolism (CsCYP707A1) and reactivation genes (CsBGs) at the transcriptional level during cucumber fruit development and ripening, in response to ABA application, dehydration and pollination, among which CsNCED1, CsCYP707A1 and CsBG1 were highly expressed in pulp and may play more important roles in regulating ABA metabolism. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  19. Effect of pectin edible coating enriched with essential oils of citrus on strawberry quality during refrigerated storage and shelf life

    Directory of Open Access Journals (Sweden)

    Soma Abdi

    2017-06-01

    Full Text Available Postharvest life of strawberry fruit is very short due to humidity and high metabolic activity. In order to extend the shelf life and quality of strawberry (cv. parous, effects of pectin coating enriched with different concentrations of lemon and orange peel essential oil were assessed at 20˚C for 6 days or 5˚C to 12 days. The experiment conducted based on a complete randomized design (CDR in 3 replications with 6 treatments including control (distilled water, pectin (1%, pectin enriched with orange peel essential oil (0.5 and 1% and pectin containing lemon peel essential oil (0.5 and 1%. Fruit quality was evaluated by marketing, weight loss, anthocyanin content, ascorbic acid content, total protein and chlorophyll of calyx every 3 days. Based on results coating fruits with citrus essential oil carried by pectin delayed the change in weight loss, total protein, anthocyanin content, maintained ascorbic acid content of fruits and also reduced chlorophyll destruction of calyx. The results showed that pectin coating containing lemon essential oil had a significant effect on the maintaining the visual quality of the fruit during the storage, delayed the weight loss and showed better results compared to the other coatings and to the control fruit. In addition, the maximum content of ascorbic acid and anthocyanin were obtained for strawberries coated with pectin containing lemon essential oil 1% after 12 days storage of fruits at 5˚C. All coatings significantly reduced weight loss of fresh strawberries during storage at 5˚C and 20˚C compared to the uncoated fruits. Results indicated that adding high concentrations (1% of orange peel essential oil into pectin coatings did not improve quality of fruits. In addition, it was found that adding orange peel essential oil to pectin coating accelerate decay of fruits. Based on results, pectin coating containing 1% lemon essential oil considered an appropriate treatment to improve shelf life and storage

  20. Apple MdACS6 Regulates Ethylene Biosynthesis During Fruit Development Involving Ethylene-Responsive Factor.

    Science.gov (United States)

    Li, Tong; Tan, Dongmei; Liu, Zhi; Jiang, Zhongyu; Wei, Yun; Zhang, Lichao; Li, Xinyue; Yuan, Hui; Wang, Aide

    2015-10-01

    Ethylene biosynthesis in plants involves different 1-aminocyclopropane-1-carboxylic acid synthase (ACS) genes. The regulation of each ACS gene during fruit development is unclear. Here, we characterized another apple (Malus×domestica) ACS gene, MdACS6. The transcript of MdACS6 was observed not only in fruits but also in other tissues. During fruit development, MdACS6 was initiated at a much earlier stage, whereas MdACS3a and MdACS1 began to be expressed at 35 d before harvest and immediateley after harvest, respectively. Moreover, the enzyme activity of MdACS6 was significantly lower than that of MdACS3a and MdACS1, accounting for the low ethylene biosynthesis in young fruits. Overexpression of MdACS6 (MdACS6-OE) by transient assay in apple showed enhanced ethylene production, and MdACS3a was induced in MdACS6-OE fruits but not in control fruits. In MdACS6 apple fruits silenced by the virus-induced gene silencing (VIGS) system (MdACS6-AN), neither ethylene production nor MdACS3a transcript was detectable. In order to explore the mechanism through which MdACS3a was induced in MdACS6-OE fruits, we investigated the expression of apple ethylene-responsive factor (ERF) genes. The results showed that the expression of MdERF2 was induced in MdACS6-OE fruits and inhibited in MdACS6-AN fruits. Yeast one-hybrid assay showed that MdERF2 protein could bind to the promoter of MdACS3a. Moreover, down-regulation of MdERF2 in apple flesh callus led to a decrease of MdACS3a expression, demonstrating the regulation of MdERF2 on MdACS3a. The mechanism through which MdACS6 regulates the action of MdACS3a was discussed. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  1. TIF film, substrates and nonfumigant soil disinfestation maintain fruit yields

    Directory of Open Access Journals (Sweden)

    Steven Fennimore

    2013-07-01

    Full Text Available A 5-year project to facilitate the adoption of strawberry production systems that do not use methyl bromide initially focused on fumigant alternatives and resulted in increased use of barrier films that reduce fumigant emissions. The focus shifted in year 3 to evaluating and demonstrating nonfumigant alternatives: soilless production, biofumigation, anaerobic soil disinfestation (ASD and disinfestation with steam. In the 2010–2011 strawberry production season, fruit yields on substrates were comparable to fruit yields using conventional methods. Anaerobic soil disinfestation and steam disinfestation also resulted in fruit yields that were comparable to those produced using conventionally fumigated soils. Additional work is in progress to evaluate their efficacy in larger-scale production systems in different strawberry production districts in California.

  2. Evaluation of Sanitizing Methods for Reducing Microbial Contamination on Fresh Strawberry, Cherry Tomato, and Red Bayberry

    Directory of Open Access Journals (Sweden)

    Wei Wei

    2017-12-01

    Full Text Available Strawberries, cherry tomatoes, and red bayberries, which are the most popular types of fresh produce in China, are vulnerable to microbial contamination. In this study, different sanitizing methods [treatment with 2% organic acids, 0.02% sodium hypochlorite (SH, 0.1% sodium chlorite (SC, and 0.1% acidified sodium chlorite (ASC] were applied to fresh strawberry, cherry tomato, and red bayberry, and their abilities to reduce aerobic bacteria, Escherichia coli O157:H7, mold, yeast, and Salmonella Typhimurium were evaluated. The commercially used SH method reduced the background microbiota on strawberry, cherry tomato, and red bayberry by 0.20–2.07 log cfu/g. The ASC method reduced background microbiota (except for mold on strawberry and cherry tomato by more than 3.0 log cfu/g. ASC was the only sanitizer that significantly reduced mold on red bayberry, and lactic acid was the only organic acid sanitizer that effectively reduced yeast on red bayberry. The ASC method had the best sterilizing effect on the three fresh fruits and also required the shortest sanitizing time and low chlorite content. The application of ASC method significantly reduced the microbiota on retail grocery samples, and the effect was similar to that achieved by sanitizing methods comparison.

  3. Anoxia Treatment for Delaying Skin Browning, Inhibiting Disease Development and Maintaining the Quality of Litchi Fruit

    Directory of Open Access Journals (Sweden)

    Yueming Jiang

    2004-01-01

    Full Text Available Litchi fruit has a very short shelf life after harvest, so marketers and consumers alike desire longer periods of storage, transportation and distribution. To extend shelf life, anoxia treatments were used for the fruit. Litchi fruit were exposed to pure N2 for 0, 3, 6, 12 or 24 h. They were then kept individually in closed but vented containers for 6 days in the dark at 20 °C and 95–100 % relative humidity. Exposure of litchi fruit to N2 for 3 or 6 h markedly delayed skin browning, reduced rot development and maintained higher concentrations of total soluble solids, titratable acidity and ascorbic acid after 6 days of storage. Anoxia treatment for 24 h reduced browning index, but it accelerated disease development, compared to the control. Thus, a pre-storage pure N2 treatment for 3 or 6 h can be an effective means of reducing rotting while maintaining the physical quality of the fruit.

  4. Development and morphology of the fruit and seed of the hemiparasite genus Jodina (Cervantesiaceae

    Directory of Open Access Journals (Sweden)

    María Luján Luna

    2017-05-01

    Full Text Available The monotypic genus Jodina is endemic to central and southeastern South America, common in forests and scrubland environments. The fruit type in Jodina is controversial since it has been described at different stages of development. The main objective of this work was to analyze the morphology of the fruit of J. rhombifolia during its maturation to attain a consensus about its type. Also characteristics of seed development and anatomy were analyzed in detail. Material was processed according to conventional techniques for LM and SEM studies. The fruit of J. rhombifolia is a pseudodrupe since the ovary is half-inferior; the fleshy layer of the pericarp is constituted by an increase of the nectary disk whereas the stony layer is represented by the mesocarp. Participation of the nectary disk as part of the fruit wall has not been mentioned previously. The seed is “naked” —the integument disintegrates during development— and the resulting structure is a pyrene. The interpretations made by other authors on the fruit of Jodina are also discussed.

  5. The transformation and translocation of 14C-labelled glucose in the course of granulation development of Batangas mandarin fruits

    International Nuclear Information System (INIS)

    Wang Xiangyang

    1996-01-01

    In the middle of storage period for Batangas mandarin fruits, the fruits segment was injected with 14 C-labelled glucose. Radioisotope was found in the rind and non-injected segments of the granulated fruits or normal fruits. The results showed that the radioactivity in the rind of slight-granulated fruits was significantly lower than that in normal fruits. The diffusion of 14 C-labelled glucose to the rind or to the segments of slightly granulated fruits was significantly higher than that of normals. These results indicated that granulation was not caused by the shift of nutritive matter from the juice sac to the rind but likely by its senescence. In the course of granulation development, the radioactivity of insoluble carbohydrate of the juice sac in severely granulated fruits was 24.65% of the total sac radioactivity. However, it was only 6.41% in normal fruits. Although the radioactivity in the rind of granulated fruits was lower than that in normal fruits, it increased faster. The respiratory rate of medium granulated fruit was 17.18 CO 2 ml/kg·h and that of severely granulated fruit was 23.64 CO 2 mol/kg·h. Both were significantly higher than that of normal fruits (11.03 CO 2 ml/kg·h). It was suggested that the sharp loss of soluble nutritive matter in the juice sac of granulated fruits might result from their transformation into insoluble matter, translocation to the rind and respiration consumption

  6. Recent developments in high-quality drying of vegetables, fruits, and aquatic products.

    Science.gov (United States)

    Zhang, Min; Chen, Huizhi; Mujumdar, Arun S; Tang, Juming; Miao, Song; Wang, Yuchuan

    2017-04-13

    Fresh foods like vegetables, fruits, and aquatic products have high water activity and they are highly heat-sensitive and easily degradable. Dehydration is one of the most common methods used to improve food shelf-life. However, drying methods used for food dehydration must not only be efficient and economic but also yield high-quality products based on flavor, nutrients, color, rehydration, uniformity, appearance, and texture. This paper reviews some new drying technologies developed for dehydration of vegetables, fruits, and aquatic products. These include: infrared drying, microwave drying, radio frequency drying, electrohydrodynamic drying, etc., as well as hybrid drying methods combining two or more different drying techniques. A comprehensive review of recent developments in high-quality drying of vegetables, fruits and aquatic products is presented and recommendations are made for future research.

  7. Identification of Optimal Reference Genes for Normalization of qPCR Analysis during Pepper Fruit Development

    Directory of Open Access Journals (Sweden)

    Yuan Cheng

    2017-06-01

    Full Text Available Due to its high sensitivity and reproducibility, quantitative real-time PCR (qPCR is practiced as a useful research tool for targeted gene expression analysis. For qPCR operations, the normalization with suitable reference genes (RGs is a crucial step that eventually determines the reliability of the obtained results. Although pepper is considered an ideal model plant for the study of non-climacteric fruit development, at present no specific RG have been developed or validated for the qPCR analyses of pepper fruit. Therefore, this study aimed to identify stably expressed genes for their potential use as RGs in pepper fruit studies. Initially, a total of 35 putative RGs were selected by mining the pepper transcriptome data sets derived from the PGP (Pepper Genome Platform and PGD (Pepper Genome Database. Their expression stabilities were further measured in a set of pepper (Capsicum annuum L. var. 007e fruit samples, which represented four different fruit developmental stages (IM: Immature; MG: Mature green; B: Break; MR: Mature red using the qPCR analysis. Then, based on the qPCR results, three different statistical algorithms, namely geNorm, Normfinder, and boxplot, were chosen to evaluate the expression stabilities of these putative RGs. It should be noted that nine genes were proven to be qualified as RGs during pepper fruit development, namely CaREV05 (CA00g79660; CaREV08 (CA06g02180; CaREV09 (CA06g05650; CaREV16 (Capana12g002666; CaREV21 (Capana10g001439; CaREV23 (Capana05g000680; CaREV26 (Capana01g002973; CaREV27 (Capana11g000123; CaREV31 (Capana04g002411; and CaREV33 (Capana08g001826. Further analysis based on geNorm suggested that the application of the two most stably expressed genes (CaREV05 and CaREV08 would provide optimal transcript normalization in the qPCR experiments. Therefore, a new and comprehensive strategy for the identification of optimal RGs was developed. This strategy allowed for the effective normalization of the q

  8. Comparative transcriptome sequencing and de novo analysis of Vaccinium corymbosum during fruit and color development.

    Science.gov (United States)

    Li, Lingli; Zhang, Hehua; Liu, Zhongshuai; Cui, Xiaoyue; Zhang, Tong; Li, Yanfang; Zhang, Lingyun

    2016-10-12

    Blueberry is an economically important fruit crop in Ericaceae family. The substantial quantities of flavonoids in blueberry have been implicated in a broad range of health benefits. However, the information regarding fruit development and flavonoid metabolites based on the transcriptome level is still limited. In the present study, the transcriptome and gene expression profiling over berry development, especially during color development were initiated. A total of approximately 13.67 Gbp of data were obtained and assembled into 186,962 transcripts and 80,836 unigenes from three stages of blueberry fruit and color development. A large number of simple sequence repeats (SSRs) and candidate genes, which are potentially involved in plant development, metabolic and hormone pathways, were identified. A total of 6429 sequences containing 8796 SSRs were characterized from 15,457 unigenes and 1763 unigenes contained more than one SSR. The expression profiles of key genes involved in anthocyanin biosynthesis were also studied. In addition, a comparison between our dataset and other published results was carried out. Our high quality reads produced in this study are an important advancement and provide a new resource for the interpretation of high-throughput data for blueberry species whether regarding sequencing data depth or species extension. The use of this transcriptome data will serve as a valuable public information database for the studies of blueberry genome and would greatly boost the research of fruit and color development, flavonoid metabolisms and regulation and breeding of more healthful blueberries.

  9. Sawdust and Bark-Based Substrates for Soilless Strawberry Production: Irrigation and Electrical Conductivity Management.

    Science.gov (United States)

    Depardieu, Claire; Prémont, Valérie; Boily, Carole; Caron, Jean

    2016-01-01

    The objective of this work was to optimize a soilless growing system for producing bare-root strawberry transplants in three organic substrates. Three trials were conducted in the Quebec City area to determine the productivity potential of a peat-sawdust mixture (PS25) and an aged bark (AB) material compared to conventional coconut fiber (CF) substrate. A first experiment was carried out to define appropriate irrigation set points for each substrate that allowed optimal plant growth and fruit yields. For all substrates, wetter conditions (irrigation started at -1.0 kPa for CF; -1.5 kPa for AB and PS25, relative to -1.5 kPa for CF; -2.5 kPa for AB and PS25) enhanced plant growth and fruit production. The second trial was carried out to test the productivity potential for commercial production of the three substrates using high-tunnels. After the addition of an initial fertilizer application to PS25, we successfully established bare-root plants that gave similar fruit yields than those in CF and AB. The productivity potential of PS25 and AB were further confirmed during a third trial under greenhouse conditions. The critical factor for plant establishment in PS25 was attributed to consistent N, P and S immobilization by microorganisms, as well as the retention of other elements (Mg2+, K+) in the growth media. Taken together, our results showed that PS25 and AB are promising alternative substrates to coconut coir dust for strawberry cultivation. This paper also provides a useful guide for strawberry cultivation in Quebec, and suggests future research that might be conducted to optimize soilless systems for cold-climate strawberry production in Northern America.

  10. Sawdust and Bark-Based Substrates for Soilless Strawberry Production: Irrigation and Electrical Conductivity Management.

    Directory of Open Access Journals (Sweden)

    Claire Depardieu

    Full Text Available The objective of this work was to optimize a soilless growing system for producing bare-root strawberry transplants in three organic substrates. Three trials were conducted in the Quebec City area to determine the productivity potential of a peat-sawdust mixture (PS25 and an aged bark (AB material compared to conventional coconut fiber (CF substrate. A first experiment was carried out to define appropriate irrigation set points for each substrate that allowed optimal plant growth and fruit yields. For all substrates, wetter conditions (irrigation started at -1.0 kPa for CF; -1.5 kPa for AB and PS25, relative to -1.5 kPa for CF; -2.5 kPa for AB and PS25 enhanced plant growth and fruit production. The second trial was carried out to test the productivity potential for commercial production of the three substrates using high-tunnels. After the addition of an initial fertilizer application to PS25, we successfully established bare-root plants that gave similar fruit yields than those in CF and AB. The productivity potential of PS25 and AB were further confirmed during a third trial under greenhouse conditions. The critical factor for plant establishment in PS25 was attributed to consistent N, P and S immobilization by microorganisms, as well as the retention of other elements (Mg2+, K+ in the growth media. Taken together, our results showed that PS25 and AB are promising alternative substrates to coconut coir dust for strawberry cultivation. This paper also provides a useful guide for strawberry cultivation in Quebec, and suggests future research that might be conducted to optimize soilless systems for cold-climate strawberry production in Northern America.

  11. Genome-wide identification of sweet orange (Citrus sinensis) histone modification gene families and their expression analysis during the fruit development and fruit-blue mold infection process.

    Science.gov (United States)

    Xu, Jidi; Xu, Haidan; Liu, Yuanlong; Wang, Xia; Xu, Qiang; Deng, Xiuxin

    2015-01-01

    In eukaryotes, histone acetylation and methylation have been known to be involved in regulating diverse developmental processes and plant defense. These histone modification events are controlled by a series of histone modification gene families. To date, there is no study regarding genome-wide characterization of histone modification related genes in citrus species. Based on the two recent sequenced sweet orange genome databases, a total of 136 CsHMs (Citrus sinensis histone modification genes), including 47 CsHMTs (histone methyltransferase genes), 23 CsHDMs (histone demethylase genes), 50 CsHATs (histone acetyltransferase genes), and 16 CsHDACs (histone deacetylase genes) were identified. These genes were categorized to 11 gene families. A comprehensive analysis of these 11 gene families was performed with chromosome locations, phylogenetic comparison, gene structures, and conserved domain compositions of proteins. In order to gain an insight into the potential roles of these genes in citrus fruit development, 42 CsHMs with high mRNA abundance in fruit tissues were selected to further analyze their expression profiles at six stages of fruit development. Interestingly, a numbers of genes were expressed highly in flesh of ripening fruit and some of them showed the increasing expression levels along with the fruit development. Furthermore, we analyzed the expression patterns of all 136 CsHMs response to the infection of blue mold (Penicillium digitatum), which is the most devastating pathogen in citrus post-harvest process. The results indicated that 20 of them showed the strong alterations of their expression levels during the fruit-pathogen infection. In conclusion, this study presents a comprehensive analysis of the histone modification gene families in sweet orange and further elucidates their behaviors during the fruit development and the blue mold infection responses.

  12. Breeding programme for developing new sweet cherry cultivars in the Fruit Growing Institute, Plovdiv, Bulgaria

    Directory of Open Access Journals (Sweden)

    S. Malchev

    2016-12-01

    Full Text Available Abstract. Sweet cherry is a major structural species in Bulgaria. According to the Ministry of Agriculture and Food, in 2010 it occupied 21% of the fruit tree areas, which defined it as a leading fruit crop. It represents 16% of the total fruit production in the country, as is the relative share of peach, being surpassed only by apple and plum production. The increased interest in establishing new cherry plantations necessitates the provision of new market-oriented cultivars with a better sensory profile of the fruits, resistant to biotic and abiotic stress factors, suitable for creating modern intensive cherry plantations. The Bulgarian sweet cherry cultivars are chronologically discussed and a thorough description of the development of the sweet cherry breeding programme, launched at the Fruit Growing Institute in Plovdiv in 1987, is presented. Current objectives comply with the world's major breeding trends and the changing market requirements. The paper reflects the main objectives of the programme and the finalized products obtained in the last twenty years of the past century and first decade of the new millennium, i.e. the new cultivars 'Kossara', 'Rosita', 'Rozalina' and 'Thrakiiska hrushtyalka' and some promising hybrids.

  13. Effect of Gamma Irradiation and Natural Antitranspirant on the Storage Ability of Strawberry

    International Nuclear Information System (INIS)

    Fath El-Bab, T.Sh.; Abdeldaiem, M.H.; Helaly, A.A.

    2013-01-01

    The study was conducted in seasons 2010 and 2011 to investigate the effect of gamma irradiation combined with natural antitranspirant of Carboxy Methyl Cellulose (CMC) on physical and chemical properties of fresh strawberries fruits ( Fragaria spp.) cv. Camarosa at the age 120 days under cold storage at (5 + 1 degree C and 85- 95 % R.h) in the Plant Research Department, Nuclear Research Center, Inshas, Egypt. The results revealed that gamma irradiation combined with natural antitranspirant (CMC) treatments were more significantly effective than the control, whereas all treatments induced less loss of weight and unmarketable fruits percentage during storage. The most effective dose decreasing the loss of weight and unmarketable percentage was 50 Gy gamma rays combined with (CMC) at 3 %. Also treatments significantly improved fruits compositions, particularly titratable acidity, total soluble solids and total sugars content, but the ascorbic acid content led to a significant decrease in strawberries fruits than the control. The best results with these fruits which treated by 50 Gy with 3% CMC combined

  14. The acquisition, development, and maintenance of fruit machine gambling in adolescents.

    Science.gov (United States)

    Griffiths, M D

    1990-09-01

    In the U.K., the major commercial gambling activity in adolescents is the playing of slot machines (commonly known as "fruit machines"). Over the past few years, the negative effects of "fruit machine addiction" have been reported by various helping organizations (e.g. Gamblers Anonymous) and the national press, including allegations of attempted murder, suicide and prostitution as well as a wider incidence of petty crime.Fifty adolescent fruit machine players from a 'user population' participated in a face-to-face interview and questionnaire study examining factors in the acquisition, development and maintenance of gambling behaviour. Nine adolescent males were deemed to be pathological gamblers as measured by the American Psychiatric Association DSM-III-R diagnostic criteria, and a number of serious consequences were reported including gambling debts, truancy and stealing.

  15. Vernalização em cinco cultivares de morangueiro Vernalization on five cultivars of strawberry

    Directory of Open Access Journals (Sweden)

    Marcelo Fontanetti Verdial

    2007-08-01

    Full Text Available A produção de morango é sazonal, e os melhores preços dos frutos são obtidos fora da estação. Buscando obter frutos fora da estação, o objetivo deste trabalho foi avaliar o efeito da vernalização em mudas de cinco cultivares de morangueiro. As mudas foram produzidas no sistema de vasos suspensos. O delineamento experimental utilizado foi blocos ao acaso em fatorial 5x2x4, com cinco cultivares ("IAC-Campinas", "Dover", "Sweet Charlie", "Cartuno" e "Oso Grande", dois tratamentos (com e sem vernalização das mudas e quatro tempos, com quatro repetições. Para vernalização, as mudas foram levadas para câmara fria à temperatura de 10+2°C e fotoperíodo de 8h de luz dia-1 durante 28 dias, e em seguida transplantadas. Foram avaliadas as percentagens de sobrevivência, florescimento, frutificação, emissão de estolhos, a massa e o número de frutos produzidos por planta. A sobrevivência de mudas não foi afetada pela vernalização. Houve efeito significativo no florescimento e na frutificação das plantas vernalizadas. O estolonamento de plantas foi mais precoce nas mudas vernalizadas. Para todas as cultivares e tratamentos, a produção de frutos foi insignificante e comercialmente inviável.The strawberry production is seasonal, and the best fruit prices are obtained during the off season. Seeking to get fruits off season, this research was aimed at evaluating the effect of the seedling vernalization of five strawberry cultivars. The strawberry seedling were produced in suspended pot system. The experimental design was is completely randomized blocks with the factorial design 5x2x4, with five strawberry cultivars (IAC-Campinas, Dover, Sweet Charlie, Cartuno and Oso Grande, two treatments (with and without strawberry runners vernalization and four times. For vernalization, the strawberry seedling was taken to cold chamber at 10±2°C, and photoperiod of 8h of light day-1 for 28 days, after this they were transplanted. The

  16. Consumer-Oriented New Product Development in Fruit Flavour Breeding - A Bayesian Approach

    NARCIS (Netherlands)

    Tesfaye, L.M.; Lans, van der I.A.; Bink, M.C.A.M.; Gremmen, H.G.J.; Trijp, van J.C.M.

    2014-01-01

    Taking consumer quality perceptions into account is very important for new-fruit product development in todays competitive food market. To this end, consumer-oriented quality improvement models like the Quality Guidance Model (QGM) have been proposed. Implementing such mod- els in the agro industry

  17. Cytokinins in the perianth, carpels, and developing fruit of Helleborus niger L

    Czech Academy of Sciences Publication Activity Database

    Tarkowski, Petr; Tarkowská, Danuše; Novák, Ondřej; Mihaljević, S.; Magnus, V.; Strnad, Miroslav; Salopek-Sondi, N.

    2006-01-01

    Roč. 57, č. 10 (2006), s. 2237-2247 ISSN 0022-0957 Grant - others:Ministry of Science, Education, and Sports of the Republic of Croatia (HR) 0098080 Institutional research plan: CEZ:AV0Z50380511 Keywords : Christmas rose * cytokinin identification and quantification * fruit and seed development Subject RIV: CE - Biochemistry Impact factor: 3.630, year: 2006

  18. Cognitive development and children's perceptions of fruit and vegetables: a qualitive study

    NARCIS (Netherlands)

    Zeinstra, G.G.; Koelen, M.A.; Kok, F.J.; Graaf, de C.

    2007-01-01

    Background Most children do not meet the recommended guidelines for fruit and vegetable intake. Since preference is an important predictor of intake, more knowledge is needed about children's preferences and about how these preferences develop. As most research about preferences has ignored

  19. Ziziphus mauritiana (masau) fruits fermentation in Zimbabwe: from black-box to starter culture development

    NARCIS (Netherlands)

    Nyanga, L.K.

    2012-01-01

    This thesis reports on studies of microbiological and biochemical properties of masau (Ziziphus mauritiana) fruit fermentation and the development of starter cultures for the production of masau beverages.

    A survey to document the traditional processing

  20. The role of Momordica balsamina fruit pulp extract in development of ...

    African Journals Online (AJOL)

    The role of Momordica balsamina fruit pulp extract in development of immunity to avian newcastle disease virus. ... The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader). If you would like more information about how to ...

  1. THE DEVELOPMENT OF PLANTS FOR THE PRODUCTION OF CONCENTRATED PASTES OF FRUIT AND VEGETABLE RAW MATERIALS

    Directory of Open Access Journals (Sweden)

    G. O. Magomedov

    2015-01-01

    Full Text Available Summary. Developed a new system for producing concentrated semi-finished products in the form of pastes for the food industry. Currently, an important task of the food industry is the creation of new products with the aim of improving the structure of the range, saving scarce raw materials, as well as reduce sugar intake; development of product functionality and products with extended shelf life. The use of local non-traditional types of plant materials can contribute to solving existing problems. Fruit and vegetable pastes are a valuable food products which can be used as a semifinished product in the confectionery, bakery, food concentrates industry. Fruit and vegetable purees have a distinct structurally viscous or pseudo-plastic properties and concentration form a very viscous mass. Already in the beginning of the process of concentration, i.e. at a relatively low degree of evaporation that leads to a rapid increase in the viscosity of the concentrate mass and reduce evaporation. With increasing temperature is the burning mass, and also change its color and flavor. Therefore, for the concentration of fruit and vegetable purees, you must use equipment whose design takes into account the possible rheological and thermal problems. The analysis of literary data structures evaporators and studies, we developed a system for producing concentrated pastes of fruit and vegetable raw materials. Developed installation can increase the quality of the finished product due to the intensification of the process of concentration, to reduce material and energy resources, increase productivity.

  2. Development of a Handmade Conductivity Measurement Apparatus and Application to Vegetables and Fruits

    Science.gov (United States)

    Set, Seng; Kita, Masakazu

    2014-01-01

    This paper describes the development of a simple handmade conductivity measurement apparatus based on a Kohlrausch bridge with inexpensive materials. We have examined the reliability of this apparatus with standard solutions and then measured juices of vegetables and fruits as well as a sports drink. Comparisons to total alkali content as…

  3. Low hanging fruit in infectious disease drug development.

    Science.gov (United States)

    Kraus, Carl N

    2008-10-01

    Cost estimates for developing new molecular entities (NME) are reaching non-sustainable levels and coupled with increasing regulatory requirements and oversight have led many pharmaceutical sponsors to divest their anti-microbial development portfolios [Projan SJ: Why is big Pharma getting out of anti-bacterial drug discovery?Curr Opin Microbiol 2003, 6:427-430] [Spellberg B, Powers JH, Brass EP, Miller LG, Edwards JE, Jr: Trends in antimicrobial drug development: implications for the future.Clin Infect Dis 2004, 38:1279-1286]. Operational issues such as study planning and execution are significant contributors to the overall cost of drug development that can benefit from the leveraging of pre-randomization data in an evidence-based approach to protocol development, site selection and patient recruitment. For non-NME products there is even greater benefit from available data resources since these data may permit smaller and shorter study programs. There are now many available open source intelligence (OSINT) resources that are being integrated into drug development programs, permitting an evidence-based or 'operational epidemiology' approach to study planning and execution.

  4. Change in chemical constituents and free radical-scavenging activity during Pear (Pyrus pyrifolia) cultivar fruit development.

    Science.gov (United States)

    Cho, Jeong-Yong; Lee, Sang-Hyun; Kim, Eun Hee; Yun, Hae Rim; Jeong, Hang Yeon; Lee, Yu Geon; Kim, Wol-Soo; Moon, Jae-Hak

    2015-01-01

    Changes in chemical constituent contents and DPPH radical-scavenging activity in fruits of pear (Pyrus pyrifolia) cultivars during the development were investigated. The fruits of seven cultivars (cv. Niitaka, Chuhwangbae, Wonhwang, Hwangkeumbae, Hwasan, Manpungbae, and Imamuraaki) were collected at 15-day intervals after day 20 of florescence. Vitamins (ascorbic acid and α-tocopherol), arbutin, chlorogenic acid, malaxinic acid, total caffeic acid, total flavonoids, and total phenolics were the highest in immature pear fruit on day 20 after florescence among samples at different growth stages. All of these compounds decreased gradually in the fruit during the development. Immature pear fruit on day 35 or 50 after florescence exhibited higher free radical-scavenging activity than that at other times, although activities were slightly different among cultivars. The chemical constituent contents and free radical-scavenging activity were largely different among immature fruits of the pear cultivars, but small differences were observed when they matured.

  5. The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco.

    Science.gov (United States)

    Aharoni, A; De Vos, C H; Wein, M; Sun, Z; Greco, R; Kroon, A; Mol, J N; O'Connell, A P

    2001-11-01

    Fruit ripening is characterized by dramatic changes in gene expression, enzymatic activities and metabolism. Although the process of ripening has been studied extensively, we still lack valuable information on how the numerous metabolic pathways are regulated and co-ordinated. In this paper we describe the characterization of FaMYB1, a ripening regulated strawberry gene member of the MYB family of transcription factors. Flowers of transgenic tobacco lines overexpressing FaMYB1 showed a severe reduction in pigmentation. A reduction in the level of cyanidin 3-rutinoside (an anthocyanin) and of quercetin-glycosides (flavonols) was observed. Expression of late flavonoid biosynthesis genes and their enzyme activities were adversely affected by FaMYB1 overexpression. Two-hybrid assays in yeast showed that FaMYB1 could interact with other known anthocyanin regulators, but it does not act as a transcriptional activator. Interestingly, the C-terminus of FaMYB1 contains the motif pdLNL(D)/(E)Lxi(G)/S. This motif is contained in a region recently proposed to be involved in the repression of transcription by AtMYB4, an Arabidopsis MYB protein. Our results suggest that FaMYB1 may play a key role in regulating the biosynthesis of anthocyanins and flavonols in strawberry. It may act to repress transcription in order to balance the levels of anthocyanin pigments produced at the latter stages of strawberry fruit maturation, and/or to regulate metabolite levels in various branches of the flavonoid biosynthetic pathway.

  6. Heat shock transcription factors expression during fruit development and under hot air stress in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit.

    Science.gov (United States)

    Lin, Qiong; Jiang, Qing; Lin, Juanying; Wang, Dengliang; Li, Shaojia; Liu, Chunrong; Sun, Chongde; Chen, Kunsong

    2015-04-01

    Heat shock transcription factors (Hsfs) play a role in plant responses to stress. Citrus is an economically important fruit whose genome has been fully sequenced. So far, no detailed characterization of the Hsf gene family is available for citrus. A genome-wide analysis was carried out in Citrus clementina to identify Hsf genes, named CcHsfs. Eighteen CcHsfs were identified and classified into three main clades (clades A, B and C) according to the structural characteristics and the phylogenetic comparison with Arabidopsis and tomato. MEME motif analysis highlighted the conserved DBD and HR-A/B domains, which were similar to Hsf protein structures in other species. Gene expression analysis in Ponkan (Citrus reticulata Blanco cv. Ponkan) fruit identified 14 Hsf genes, named CrHsf, as important candidates for a role in fruit development and ripening, and showed seven genes to be expressed in response to hot air stress. CrHsfB2a and CrHsfB5 were considered to be important regulators of citrate content and showed variation in both developmentally-related and hot air-triggered citrate degradation processes. In summary, the data obtained from this investigation provides the basis for further study to dissect Hsf function during fruit development as well as in response to heat stress and also emphasizes the potential importance of CrHsfs in regulation of citrate metabolism in citrus fruit. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Ultrasound improves chemical reduction of natural contaminant microbiota and Salmonella enterica subsp. enterica on strawberries.

    Science.gov (United States)

    do Rosário, Denes Kaic Alves; da Silva Mutz, Yhan; Peixoto, Jaqueline Moreira Curtis; Oliveira, Syllas Borburema Silva; de Carvalho, Raquel Vieira; Carneiro, Joel Camilo Souza; de São José, Jackline Freitas Brilhante; Bernardes, Patrícia Campos

    2017-01-16

    New sanitization methods have been evaluated to improve food safety and food quality and to replace chlorine compounds. However, these new methods can lead to physicochemical and sensory changes in fruits and vegetables. The present study evaluated the effects of acetic acid, peracetic acid, and sodium dodecylbenzenesulfonate isolated or combined with 5min of ultrasound treatment (40kHz, 500W) on strawberry quality over 9days of storage at 8°C. The strawberry natural contaminant microbiota (molds and yeasts, mesophilic aerobic and lactic acid bacteria), physicochemical quality (pH, total titratable acidity, total soluble solids, vitamin C, and color), sensory quality (triangle test) and inactivation of Salmonella enterica subsp. enterica intentionally inoculated onto strawberries were analyzed. Ultrasound increased the effect of all chemical compounds in the reduction of aerobic mesophilic, molds and yeasts. The best treatment for those groups of microorganisms was ultrasound combined with peracetic acid (US+PA) that reduced 1.8 and 2.0logcfu/g during 9days of storage. Bactericidal effect of peracetic acid was also improved by ultrasound inactivation of S. enterica, reaching a decimal reduction of 2.1logcfu/g. Moreover, synergistic effects were observed in contaminant natural microbiota inactivation for all tested compounds during storage, without any major physicochemical or sensory alteration to the strawberries. Therefore, ultrasound treatment can improve the effect of sanitizers that are substitutes of chlorine compounds without altering the quality of strawberries during storage. Acetic acid (PubChem CID: 176); Peracetic acid (PubChem CID: 6585); Sodium dodecylbenzenesulfonate (PubChem CID: 18372154). Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Report on the development of putative functional SSR and SNP markers in passion fruits.

    Science.gov (United States)

    da Costa, Zirlane Portugal; Munhoz, Carla de Freitas; Vieira, Maria Lucia Carneiro

    2017-09-06

    Passionflowers Passiflora edulis and Passiflora alata are diploid, outcrossing and understudied fruit bearing species. In Brazil, passion fruit cultivation began relatively recently and has earned the country an outstanding position as the world's top producer of passion fruit. The fruit's main economic value lies in the production of juice, an essential exotic ingredient in juice blends. Currently, crop improvement strategies, including those for underexploited tropical species, tend to incorporate molecular genetic approaches. In this study, we examined a set of P. edulis transcripts expressed in response to infection by Xanthomonas axonopodis, (the passion fruit's main bacterial pathogen that attacks the vines), aiming at the development of putative functional markers, i.e. SSRs (simple sequence repeats) and SNPs (single nucleotide polymorphisms). A total of 210 microsatellites were found in 998 sequences, and trinucleotide repeats were found to be the most frequent (31.4%). Of the sequences selected for designing primers, 80.9% could be used to develop SSR markers, and 60.6% SNP markers for P. alata. SNPs were all biallelic and found within 15 gene fragments of P. alata. Overall, gene fragments generated 10,003 bp. SNP frequency was estimated as one SNP every 294 bp. Polymorphism rates revealed by SSR and SNP loci were 29.4 and 53.6%, respectively. Passiflora edulis transcripts were useful for the development of putative functional markers for P. alata, suggesting a certain level of sequence conservation between these cultivated species. The markers developed herein could be used for genetic mapping purposes and also in diversity studies.

  9. Fruit development, pigmentation and biochemical properties of wax apple as affected by localized Application of GA3 under field conditions

    Directory of Open Access Journals (Sweden)

    Mohammad Moneruzzaman Khandaker

    2013-02-01

    Full Text Available This study investigated the effects of gibberellin (GA3 on the fruit development, pigmentation and biochemical properties of wax apple. The wax apple trees were rubbing treated with 0, 20, 50 and 100 mgGA3/l under field conditions. The localized application (rubbing of 50 mg GA3/l significantly increased the fruit set, fruit length and diameter, color development, weight and yieldcompared to the control. In addition, GA3 treatments significantly reduced the fruit drop. With regard to the fruit quality, 50 mg/l GA3 treatment increased the juice content, K+, TSS, total sugar and sugar acid ratio of wax apple fruits. In addition, higher vitamin C, phenol, flavonoid, anthocyanin, carotene content, PAL and antioxidant activities were recorded in the treated fruits. There was a positive correlation between the peel colour and TSS content and between the PAL activity and anthocyanin formation in the GA3-treated fruit. It was concluded that rubbing with 50 mg/L GA3 at inflorescence developing point of phloem once a week from the tiny inflorescence bud until the flower opening resulted in better yield and quality of wax apple fruits and could be an effective technique to safe the environment from excessive spray.

  10. Influence of mist-chilling on post-harvest quality of fresh strawberries Cv. Mara des Bois and Gariguette

    Energy Technology Data Exchange (ETDEWEB)

    Allais, Irene [Cemagref, UMR Genial, 24, avenue des Landais B.P. 50085, 63172 Aubiere Cedex (France); Letang, Guy [Cemagref, UMR Genial, Parc de Tourvoie, B.P. 44, 92163 Antony Cedex (France)

    2009-09-15

    The aim of this study was to assess the impact of mist-chilling on high-grade strawberry post-harvest quality (Cultivars ''Gariguette'' and ''Mara des Bois''). Strawberries were chilled at 2 C using three processes: air blast chilling at 0.3 m s{sup -1} or 1 m s{sup -1} and mist-chilling at 1 m s{sup -1}. After chilling, fruits were submitted to different distribution chains characterised by different handling conditions and storage temperatures (2 C or 7 C) and by a 12 h retailing step at 20 C. Strawberry quality was assessed by measuring 7 parameters: weight loss, commercial loss, firmness, sugar content, acidity, colour and sensory quality. Compared to air-chilling, mist-chilling did not reduce chilling time but it reduced weight loss by 20-40%. Mist-chilling had no detrimental effect on commercial loss defined as the percentage of fruit more than 1/3 of surface affected. It did not induce any major changes on strawberry quality. Temperature fluctuations undergone during cold storage and retailing had a detrimental effect on weight loss. The beneficial effect of packaging on weight loss was confirmed. (author)

  11. Fine-tuning of the flavonoid and monolignol pathways during apple early fruit development.

    Science.gov (United States)

    Baldi, Paolo; Moser, Mirko; Brilli, Matteo; Vrhovsek, Urska; Pindo, Massimo; Si-Ammour, Azeddine

    2017-05-01

    A coordinated regulation of different branches of the flavonoid pathway was highlighted that may contribute to elucidate the role of this important class of compounds during the early stages of apple fruit development. Apple (Malus × domestica Borkh.) is an economically important fruit appreciated for its organoleptic characteristics and its benefits for human health. The first stages after fruit set represent a very important and still poorly characterized developmental process. To enable the profiling of genes involved in apple early fruit development, we combined the suppression subtractive hybridization (SSH) protocol to next-generation sequencing. We identified and characterized genes induced and repressed during fruit development in the apple cultivar 'Golden Delicious'. Our results showed an opposite regulation of genes coding for enzymes belonging to flavonoid and monolignol pathways, with a strong induction of the former and a simultaneous repression of the latter. Two isoforms of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase, key enzymes located at the branching point between flavonoid and monolignol pathways, showed opposite expression patterns during the period in analysis, suggesting a possible regulation mechanism. A targeted metabolomic analysis supported the SSH results and revealed an accumulation of the monomers catechin and epicatechin as well as several forms of procyanidin oligomers in apple fruitlets starting early after anthesis, together with a decreased production of other classes of flavonoids such as some flavonols and the dihydrochalcone phlorizin. Moreover, gene expression and metabolites accumulation of 'Golden Delicious' were compared to a wild apple genotype of Manchurian crabapple (Malus mandshurica (Maxim.) Kom.). Significant differences in both gene expression and metabolites accumulation were found between the two genotypes.

  12. The complete genome sequence of a new polerovirus in strawberry plants from eastern Canada showing strawberry decline symptoms.

    Science.gov (United States)

    Xiang, Yu; Bernardy, Mike; Bhagwat, Basdeo; Wiersma, Paul A; DeYoung, Robyn; Bouthillier, Michel

    2015-02-01

    Strawberry decline disease, probably caused by synergistic reactions of mixed virus infections, threatens the North American strawberry industry. Deep sequencing of strawberry plant samples from eastern Canada resulted in the identification of a new virus genome resembling poleroviruses in sequence and genome structure. Phylogenetic analysis suggests that it is a new member of the genus Polerovirus, family Luteoviridae. The virus is tentatively named "strawberry polerovirus 1" (SPV1).

  13. Chilean Strawberry Consumption Protects against LPS-Induced Liver Injury by Anti-Inflammatory and Antioxidant Capability in Sprague-Dawley Rats

    Directory of Open Access Journals (Sweden)

    Sebastian Molinett

    2015-01-01

    Full Text Available The Chilean strawberry fruit has high content of antioxidants and polyphenols. Previous studies evidenced antioxidant properties by in vitro methods. However, the antioxidant effect and its impact as functional food on animal health have not been evaluated. In this study, rats were fed with a Chilean strawberry aqueous extract (4 g/kg of animal per day and then subjected to LPS-induced liver injury (5 mg/kg. Transaminases and histological studies revealed a reduction in liver injury in rats fed with strawberry aqueous extract compared with the control group. Additionally, white strawberry supplementation significantly reduced the serum levels and gene expression of TNF-α, IL-6, and IL-1β cytokines compared with nonsupplemented rats. The level of F2-isoprostanes and GSH/GSSG indicated a reduction in liver oxidative stress by the consumption of strawberry aqueous extract. Altogether, the evidence suggests that dietary supplementation of rats with a Chilean white strawberry aqueous extract favours the normalization of oxidative and inflammatory responses after a liver injury induced by LPS.

  14. Chilean Strawberry Consumption Protects against LPS-Induced Liver Injury by Anti-Inflammatory and Antioxidant Capability in Sprague-Dawley Rats.

    Science.gov (United States)

    Molinett, Sebastian; Nuñez, Francisca; Moya-León, María Alejandra; Zúñiga-Hernández, Jessica

    2015-01-01

    The Chilean strawberry fruit has high content of antioxidants and polyphenols. Previous studies evidenced antioxidant properties by in vitro methods. However, the antioxidant effect and its impact as functional food on animal health have not been evaluated. In this study, rats were fed with a Chilean strawberry aqueous extract (4 g/kg of animal per day) and then subjected to LPS-induced liver injury (5 mg/kg). Transaminases and histological studies revealed a reduction in liver injury in rats fed with strawberry aqueous extract compared with the control group. Additionally, white strawberry supplementation significantly reduced the serum levels and gene expression of TNF-α, IL-6, and IL-1β cytokines compared with nonsupplemented rats. The level of F2-isoprostanes and GSH/GSSG indicated a reduction in liver oxidative stress by the consumption of strawberry aqueous extract. Altogether, the evidence suggests that dietary supplementation of rats with a Chilean white strawberry aqueous extract favours the normalization of oxidative and inflammatory responses after a liver injury induced by LPS.

  15. Development of iodimetric redox method for routine estimation of ascorbic acid from fresh fruit and vegetables

    International Nuclear Information System (INIS)

    Munir, M.; Baloch, A. K.; Khan, W. A.; Ahmad, F.; Jamil, M.

    2013-01-01

    The iodimetric method (Im) is developed for rapid estimation of ascorbic acid from fresh fruit and vegetables. The efficiency of Im was compared with standard with standard dye method (Dm) utilizing a variety of model solutions and aqueous extracts from fresh fruit and vegetables of different colors. The Im presented consistently accurate and precise results from colorless to colored model solutions and from fruit/vegetable extracts with standard deviation (Stdev) in the range of +-0.013 - +-0.405 and +-0.019 - +-0.428 respectively with no significant difference between the replicates. The Dm worked also satisfactorily for colorless model solutions and extracts (Stdev range +-0.235 - +-0.309) while producing unsatisfactory results (+-0.464 - +-3.281) for colored counterparts. Severe discrepancies/ overestimates continued to pileup (52% to 197%) estimating the nutrient from high (3.0 mg/10mL) to low (0.5 mg/10mL) concentration levels, respectively. On the basis of precision and reliability, the Im technique is suggested for adoption in general laboratories for routine estimation of ascorbic acid from fruit and vegetables possessing any shade. (author)

  16. Antisense repression of sucrose phosphate synthase in transgenic muskmelon alters plant growth and fruit development

    International Nuclear Information System (INIS)

    Tian, Hongmei; Ma, Leyuan; Zhao, Cong; Hao, Hui; Gong, Biao; Yu, Xiyan; Wang, Xiufeng

    2010-01-01

    To unravel the roles of sucrose phosphate synthase (SPS) in muskmelon (Cucumis melo L.), we reduced its activity in transgenic muskmelon plants by an antisense approach. For this purpose, an 830 bp cDNA fragment of muskmelon sucrose phosphate synthase was expressed in antisense orientation behind the 35S promoter of the cauliflower mosaic virus. The phenotype of the antisense plants clearly differed from that of control plants. The transgenic plant leaves were markedly smaller, and the plant height and stem diameter were obviously shorter and thinner. Transmission electron microscope observation revealed that the membrane degradation of chloroplast happened in transgenic leaves and the numbers of grana and grana lamella in the chloroplast were significantly less, suggesting that the slow growth and weaker phenotype of transgenic plants may be due to the damage of the chloroplast ultrastructure, which in turn results in the decrease of the net photosynthetic rate. The sucrose concentration and levels of sucrose phosphate synthase decreased in transgenic mature fruit, and the fruit size was smaller than the control fruit. Together, our results suggest that sucrose phosphate synthase may play an important role in regulating the muskmelon plant growth and fruit development.

  17. Development of a polyprobe to detect six viroids of pome and stone fruit trees.

    Science.gov (United States)

    Lin, Liming; Li, Ruhui; Mock, Ray; Kinard, Gary

    2011-01-01

    A simple and sensitive dot blot hybridization assay using a digoxigenin-labeled cRNA polyprobe was developed for the simultaneous detection of six viroids that infect pome and stone fruit trees. The polyprobe was constructed by cloning sequentially partial sequences of each viroid into a single vector, with run-off transcription driven by the T7 promoter. All six viroids were detectable within a dilution range of 5(-3) to 5(-4) in total nucleic acid extracts from infected trees. Individual trees were co-inoculated to create mixed infections and all four pome fruit viroids and both stone fruit viroids could be detected in pear and peach trees, respectively, using the polyprobe. The results of the assays using the polyprobe were comparable to those using single probes. The methods were validated by testing geographically diverse isolates of viroids, as well as field samples from several collections in the US. The assay offers a rapid, reliable and cost-effective approach to the simultaneous detection of six fruit trees viroids and has the potential for routine use in quarantine, certification, and plant genebank programs where many samples are tested and distributed worldwide. Published by Elsevier B.V.

  18. Antisense repression of sucrose phosphate synthase in transgenic muskmelon alters plant growth and fruit development

    Energy Technology Data Exchange (ETDEWEB)

    Tian, Hongmei; Ma, Leyuan; Zhao, Cong; Hao, Hui; Gong, Biao [College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai' an, Shandong 271018 (China); Yu, Xiyan, E-mail: yuxiyan@sdau.edu.cn [College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai' an, Shandong 271018 (China); Wang, Xiufeng, E-mail: xfwang@sdau.edu.cn [College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai' an, Shandong 271018 (China)

    2010-03-12

    To unravel the roles of sucrose phosphate synthase (SPS) in muskmelon (Cucumis melo L.), we reduced its activity in transgenic muskmelon plants by an antisense approach. For this purpose, an 830 bp cDNA fragment of muskmelon sucrose phosphate synthase was expressed in antisense orientation behind the 35S promoter of the cauliflower mosaic virus. The phenotype of the antisense plants clearly differed from that of control plants. The transgenic plant leaves were markedly smaller, and the plant height and stem diameter were obviously shorter and thinner. Transmission electron microscope observation revealed that the membrane degradation of chloroplast happened in transgenic leaves and the numbers of grana and grana lamella in the chloroplast were significantly less, suggesting that the slow growth and weaker phenotype of transgenic plants may be due to the damage of the chloroplast ultrastructure, which in turn results in the decrease of the net photosynthetic rate. The sucrose concentration and levels of sucrose phosphate synthase decreased in transgenic mature fruit, and the fruit size was smaller than the control fruit. Together, our results suggest that sucrose phosphate synthase may play an important role in regulating the muskmelon plant growth and fruit development.

  19. Influência do sistema de produção e do armazenamento refrigerado nas características físico-químicas e no desenvolvimento de compostos voláteis em morangos Influence of planting system and cold storage on the physical-chemistries characteristicsand volatile compounds development in strawberries

    Directory of Open Access Journals (Sweden)

    Julia Maria Machado de Ávila

    2012-12-01

    Full Text Available Durante o armazenamento, pode ocorrer a perda de compostos voláteis responsáveis pelo aroma e o aumento de compostos indesejáveis, o que ocasiona a perda de qualidade do morango, reduzindo a vida de prateleira e levando à rejeição do produto pelos consumidores. Neste trabalho, foi avaliada a influência do sistema de produção (convencional e orgânico e do período de armazenamento (0, 2, 5, 8 dias nas características físicas e químicas (CFQ e nos compostos voláteis (CV de morango das cultivares 'Camarosa' e 'Camino Real', utilizando morangos de pomar comercial da região de Pelotas/RS. Os frutos foram colhidos em estádio de maturação comercial e com boa aparência. O planejamento experimental foi casualizado, em triplicata de 15 frutos. Os morangos foram armazenados a T de 1°C, com 90-95% umidade relativa (UR. Foram avaliadas a perda de massa, a coloração instrumental [L*, a*, b* e ângulo hue (H] , a firmeza, os sólidos solúveis (SS, a acidez titulável (AT, a relação SS/AT, pH e os teores de CV. Os resultados foram submetidos à análise de variância e as diferenças significativas determinadas pelo teste de Tukey (PDuring the storage a loss of volatile compounds responsible for the aroma and an increase of undesirable compounds can occur leading to a decrease in the strawberry quality, a reduction of the shelf life and product rejection by the consumers. In the present work the influence of the planting system (conventional and organic and the storage period (0, 2, 5 and 8 days upon physical and chemical characteristics (FCC as well as on strawberry volatile compounds (VC in the cultivars 'Camarosa' and 'Camino Real' were evaluated using strawberries from a commercial orchard from Pelotas/RS. The fruits were harvested in a stage of commercial maturation and sound appearance. The experimental planning was randomized, in triplicate of fifteen fruits. The strawberries were stored at 1°C and 90-95% of relative humidity

  20. Interactions among spectral components of radiation in the growth responses of rice, tomato and strawberry

    International Nuclear Information System (INIS)

    Inada, K.; Matsuno, A.

    1985-01-01

    Effects of spectral components and their ratios of radiation on simultaneous growth responses were investigated with rice, tomato and strawberry plants exposed to lights with a high fluence rate (350 or 408 μmol m -2 s -1 , 400-700 nm) during every daytime. Both elongation growth and Ieaf area development in rice and strawberry were promoted by red (R) but inhibited by blue (B) component depending on the each fluence rate. However, leaf area in tomato responded in opposite direction to these. The elongation growth was remarkably increased with the fluence rate of far-red (FR) in tomato and strawberry, but not in rice. These responses were lineary increased, except FR and UV effects, with logarithmic R/B ratio in rice and strawberry but not in tomato. A very low R/FR ratio caused a strong promotion of both elongation and leaf area in tomato, while it promoted petiole elengation but inhibited leaf area development in strawberry. The elongation and leaf area development responded to R/FR in reverse way between rice and strawberry. Chlorophyll content of leaves was generally decreased with the increase of logarithmic R/B ratio in all the species. Areal weight of leaf and dry weight increment/leaf area were more or less increased with R/B and R/ FR ratios, Dry weight increment varied with the spectral ratios in almost the same way as leaf area, suggesting that spectral dependence of photosynthetic production was not much different between the species. Some discussions were made on the photoreceptor pigments involved in the elongation growth and leaf area development, and on the selection of light quantity to ensure a normal growth of each plant species

  1. Genomic rearrangements and signatures of breeding in the allo-octoploid strawberry as revealed through an allele dose based SSR linkage map

    NARCIS (Netherlands)

    Dijk, van T.; Pagliarani, G.; Pikunova, A.; Noordijk, Y.; Yilmaz-Temel, H.; Meulenbroek, B.; Visser, R.G.F.; Weg, van de W.E.

    2014-01-01

    Background Breeders in the allo-octoploid strawberry currently make little use of molecular marker tools. As a first step of a QTL discovery project on fruit quality traits and resistance to soil-borne pathogens such as Phytophthora cactorum and Verticillium we built a genome-wide SSR linkage map

  2. Metagenomic analysis of fungal diversity on strawberry plants and the effect of management practices on the fungal community structure of aerial organs

    Science.gov (United States)

    Metabarcoding, defined as Next Generation Sequencing (NGS) of amplicons of the ITS2 region (DNA barcode), was used to identify the composition of the fungal community on different strawberry organs i.e. leaves, flowers, and immature and mature fruits grown on a farm using disease and insect control ...

  3. Development of Phytophthora fruit rot caused by Phytophthora capsici on resistant and susceptible watermelon fruit of different ages

    Science.gov (United States)

    Watermelon is an important crop grown in 44 states in the United States. Phytophthora fruit rot caused by Phytophthora capsici is a serious disease in the southeastern U.S., where over 50% of the watermelons are produced. The disease has resulted in severe losses to watermelon growers, especially in...

  4. Waste materials derived bio-effectors used as growth promoters for strawberry plants. An agronomic and metabolomic study

    Science.gov (United States)

    Vasileva, Brankica; Chami, Ziad Al; De Pascali, Sandra; Cavoski, Ivana; Fanizzi, Francesco Paolo

    2015-04-01

    Recently, a novel concept of bio-effectors has emerged to describe a group of products that are able to improve plant performance more than fertilizers. In this study, three different agro-industrial residues, i.e. brewers' spent grain (BSG), fennel processing residues (FPR) and lemon processing residues (LPR) were chosen as potential bio-effectors. A greenhouse soilless pot experiment was conducted on strawberry plants (Fragaria x ananassa var. Festival) in order to study the effect of BSG, FPR and LPR water extracts, at different concentrations, on plant growth and fruit quality. Their effect was compared with humic-like substances as a positive/reference control (Ctrl+) and with Hoagland solution as a negative control (Ctrl-). Agronomic parameters and the nutrient uptake were measured on shoots, roots and fruits. Metabolomic profiling tests were carried out on leaves, roots and fruit juices through the NMR technique. Plants treated with the FPR extract showed better vegetative growth, while plants treated with the BSG extract gave higher yield and better fruit size. Metabolomic profiling showed that fruits and roots of plants treated with FPR and LPR extracts had higher concentrations of sucrose, malate and acetate, while BSG treated plants had higher concentrations of citrate and β-glucose. In conclusion, according to the results achieved, the bio-effectors used in this study promote plant growth and fruit quality regardless of their nutritional content. Keywords: bio-effectors, agro-industrial waste, nuclear magnetic resonance (NMR), strawberry, growth promotion, fruit quality.

  5. The distribution of fruit and seed toxicity during development for eleven neotropical trees and vines in Central Panama.

    Science.gov (United States)

    Beckman, Noelle G

    2013-01-01

    Secondary compounds in fruit mediate interactions with natural enemies and seed dispersers, influencing plant survival and species distributions. The functions of secondary metabolites in plant defenses have been well-studied in green tissues, but not in reproductive structures of plants. In this study, the distribution of toxicity within plants was quantified and its influence on seed survival was determined in Central Panama. To investigate patterns of allocation to chemical defenses and shifts in allocation with fruit development, I quantified variation in toxicity between immature and mature fruit and between the seed and pericarp for eleven species. Toxicity of seed and pericarp was compared to leaf toxicity for five species. Toxicity was measured as reduced hyphal growth of two fungal pathogens, Phoma sp. and Fusarium sp., and reduced survivorship of brine shrimp, Artemia franciscana, across a range of concentrations of crude extract. I used these measures of potential toxicity against generalist natural enemies to examine the effect of fruit toxicity on reductions of fruit development and seed survival by vertebrates, invertebrates, and pathogens measured for seven species in a natural enemy removal experiment. The seed or pericarp of all vertebrate- and wind-dispersed species reduced Artemia survivorship and hyphal growth of Fusarium during the immature and mature stages. Only mature fruit of two vertebrate-dispersed species reduced hyphal growth of Phoma. Predispersal seed survival increased with toxicity of immature fruit to Artemia during germination and decreased with toxicity to fungi during fruit development. This study suggests that fruit toxicity against generalist natural enemies may be common in Central Panama. These results support the hypothesis that secondary metabolites in fruit have adaptive value and are important in the evolution of fruit-frugivore interactions.

  6. The distribution of fruit and seed toxicity during development for eleven neotropical trees and vines in Central Panama.

    Directory of Open Access Journals (Sweden)

    Noelle G Beckman

    Full Text Available Secondary compounds in fruit mediate interactions with natural enemies and seed dispersers, influencing plant survival and species distributions. The functions of secondary metabolites in plant defenses have been well-studied in green tissues, but not in reproductive structures of plants. In this study, the distribution of toxicity within plants was quantified and its influence on seed survival was determined in Central Panama. To investigate patterns of allocation to chemical defenses and shifts in allocation with fruit development, I quantified variation in toxicity between immature and mature fruit and between the seed and pericarp for eleven species. Toxicity of seed and pericarp was compared to leaf toxicity for five species. Toxicity was measured as reduced hyphal growth of two fungal pathogens, Phoma sp. and Fusarium sp., and reduced survivorship of brine shrimp, Artemia franciscana, across a range of concentrations of crude extract. I used these measures of potential toxicity against generalist natural enemies to examine the effect of fruit toxicity on reductions of fruit development and seed survival by vertebrates, invertebrates, and pathogens measured for seven species in a natural enemy removal experiment. The seed or pericarp of all vertebrate- and wind-dispersed species reduced Artemia survivorship and hyphal growth of Fusarium during the immature and mature stages. Only mature fruit of two vertebrate-dispersed species reduced hyphal growth of Phoma. Predispersal seed survival increased with toxicity of immature fruit to Artemia during germination and decreased with toxicity to fungi during fruit development. This study suggests that fruit toxicity against generalist natural enemies may be common in Central Panama. These results support the hypothesis that secondary metabolites in fruit have adaptive value and are important in the evolution of fruit-frugivore interactions.

  7. Profiling microRNA expression during multi-staged date palm (Phoenix dactylifera L.) fruit development

    KAUST Repository

    Xin, Chengqi; Liu, Wanfei; Lin, Qiang; Zhang, Xiaowei; Cui, Peng; Li, Fusen; Zhang, Guangyu; Pan, Linlin; Al-Amer, Ali; Mei, Hailiang; Al-Mssallem, Ibrahim S.; Hu, Songnian; Al-Johi, Hasan Awad; Yu, Jun

    2015-01-01

    MicroRNAs (miRNAs) play crucial roles in multiple stages of plant development and regulate gene expression at posttranscriptional and translational levels. In this study, we first identified 238 conserved miRNAs in date palm (Phoenix dactylifera) based on a high-quality genome assembly and defined 78 fruit-development-associated (FDA) miRNAs, whose expression profiles are variable at different fruit development stages. Using experimental data, we subsequently detected 276 novel P. dactylifera-specific FDA miRNAs and predicted their targets. We also revealed that FDA miRNAs function mainly in regulating genes involved in starch/sucrose metabolisms and other carbon metabolic pathways; among them, 221 FDA miRNAs exhibit negative correlation with their corresponding targets, which suggests their direct regulatory roles on mRNA targets. Our data define a comprehensive set of conserved and novel FDA miRNAs along with their expression profiles, which provide a basis for further experimentation in assigning discrete functions of these miRNAs in P. dactylifera fruit development.

  8. Profiling microRNA expression during multi-staged date palm (Phoenix dactylifera L.) fruit development

    KAUST Repository

    Xin, Chengqi

    2015-01-29

    MicroRNAs (miRNAs) play crucial roles in multiple stages of plant development and regulate gene expression at posttranscriptional and translational levels. In this study, we first identified 238 conserved miRNAs in date palm (Phoenix dactylifera) based on a high-quality genome assembly and defined 78 fruit-development-associated (FDA) miRNAs, whose expression profiles are variable at different fruit development stages. Using experimental data, we subsequently detected 276 novel P. dactylifera-specific FDA miRNAs and predicted their targets. We also revealed that FDA miRNAs function mainly in regulating genes involved in starch/sucrose metabolisms and other carbon metabolic pathways; among them, 221 FDA miRNAs exhibit negative correlation with their corresponding targets, which suggests their direct regulatory roles on mRNA targets. Our data define a comprehensive set of conserved and novel FDA miRNAs along with their expression profiles, which provide a basis for further experimentation in assigning discrete functions of these miRNAs in P. dactylifera fruit development.

  9. Tourist orchards: an opportunity for sustainable development tourism in Romanian traditional fruit growing areas

    Directory of Open Access Journals (Sweden)

    Albu, R. G.

    2012-01-01

    Full Text Available The Romanian traditional fruit growing areas are spaces that, given the transition from a centralized economy to a market economy, have accumulated multiple dysfunctions, whose solving requires the identification of viable solutions, matching local potential and preserving traditional specificity at the same time. The article analyzes how the tourist orchard model, developed successfully in China could be implemented in Romanian traditional fruit growing areas. Also the evolution of the Chinese tourist orchard is presented, highlighting the stages of its development and paying special attention to the current stage that witnesses the solid consolidation of the extant niche of tourist orchards, as part of the Chinese tourism. This consolidation has led to several large metropolitan areas (Beijing, Shanghai, Guangzhou and Shenzhen in China standing out on the international market.

  10. Bioinformatic Analysis of Strawberry GSTF12 Gene

    Science.gov (United States)

    Wang, Xiran; Jiang, Leiyu; Tang, Haoru

    2018-01-01

    GSTF12 has always been known as a key factor of proanthocyanins accumulate in plant testa. Through bioinformatics analysis of the nucleotide and encoded protein sequence of GSTF12, it is more advantageous to the study of genes related to anthocyanin biosynthesis accumulation pathway. Therefore, we chosen GSTF12 gene of 11 kinds species, downloaded their nucleotide and protein sequence from NCBI as the research object, found strawberry GSTF12 gene via bioinformation analyse, constructed phylogenetic tree. At the same time, we analysed the strawberry GSTF12 gene of physical and chemical properties and its protein structure and so on. The phylogenetic tree showed that Strawberry and petunia were closest relative. By the protein prediction, we found that the protein owed one proper signal peptide without obvious transmembrane regions.

  11. Shallot aphids, Myzus ascalonicus, in strawberry

    DEFF Research Database (Denmark)

    Enkegaard, Annie; Sigsgaard, Lene; Kristensen, Kristian

    2013-01-01

    The parasitization capacity of 3 parasitoids and the predation capacity of 3 predators towards the shallot aphid, Myzus ascalonicus Doncaster (Homoptera: Aphididae), on strawberry, Fragaria x ananassa Duchesne (Rosales: Rosaceae) cv. Honeoye, were examined in laboratory experiments. In Petri dish...... other parasitoid-induced causes. However, the host feeding rate was reduced to only 1.2 ± 0.8%, and no significant parasitization mortality was observed on strawberry plants, suggesting that host plants interfered with A. abdominalis activity. This parasitoid does not, therefore, seem to be suited...... to either inoculative or inundative biocontrol of shallot aphids in strawberry. The three predators studied were the green lacewing, Chrysoperla carnea Steph. (Neuroptera: Chrysopi-dae), the two-spotted lady beetle, Adalia bipunctata L. (Coleoptera: Coccinellidae), and the gall midge Aphidoletes aphidimyza...

  12. Exposure to environmentally-relevant levels of ozone negatively influence pollen and fruit development

    International Nuclear Information System (INIS)

    Gillespie, Colin; Stabler, Daniel; Tallentire, Eva; Goumenaki, Eleni; Barnes, Jeremy

    2015-01-01

    A combination of in vitro and in vivo studies on tomato (Lycopersicon esculentum Mill. cv. Triton) revealed that environmentally-relevant levels of ozone (O_3) pollution adversely affected pollen germination, germ tube growth and pollen-stigma interactions – pollen originating from plants raised in charcoal-Purafil"® filtered air (CFA) exhibited reduced germ tube development on the stigma of plants exposed to environmentally-relevant levels of O_3. The O_3-induced decline in in vivo pollen viability was reflected in increased numbers of non-fertilized and fertilized non-viable ovules in immature fruit. Negative effects of O_3 on fertilization occurred regardless of the timing of exposure, with reductions in ovule viability evident in O_3 × CFA and CFA × O_3 crossed plants. This suggests O_3-induced reductions in fertilization were associated with reduced pollen viability and/or ovule development. Fruit born on trusses independently exposed to 100 nmol mol"−"1 O_3 (10 h d"−"1) from flowering exhibited a decline in seed number and this was reflected in a marked decline in the weight and size of individual fruit – a clear demonstration of the direct consequence of the effects of the pollutant on reproductive processes. Ozone exposure also resulted in shifts in the starch and ascorbic acid (Vitamin C) content of fruit that were consistent with accelerated ripening. The findings of this study draw attention to the need for greater consideration of, and possibly the adoption of weightings for the direct impacts of O_3, and potentially other gaseous pollutants, on reproductive biology during ‘risk assessment’ exercises. - Highlights: • Environmentally-relevant levels of ozone exert negative effects on pollen viability that translate into direct impacts on fruit yield. • Negative impacts of O_3 pollution on reproductive processes were evident regardless of the stage of plant development. • Exposure to environmentally-relevant levels of O_3

  13. Characterization of fruit development and potential health benefits of arrayan (Luma apiculata), a native berry of South America.

    Science.gov (United States)

    Fuentes, Lida; Valdenegro, Mónika; Gómez, María-Graciela; Ayala-Raso, Aníbal; Quiroga, Evelyn; Martínez, Juan-Pablo; Vinet, Raúl; Caballero, Eduardo; Figueroa, Carlos R

    2016-04-01

    The arrayan berry (Luma apiculata) is a native fruit from South America that belongs to the Myrtaceae family. To elucidate and characterize the developmental process and the potential health benefits of this edible fruit, quality and physiological parameters, along with antioxidant capacity, were evaluated during four clearly defined developmental stages of the fruit in two seasons. Fruit firmness slowly decreases during fruit development, whereas the solid soluble content/titratable acidity ratio (SSC/TA) increases significantly in the final stages of development. The measurement of low respiration rates and low ethylene production during growth and ripening suggested that the arrayan berry should be classified as a non-climacteric fruit. Arrayan berries show a significant increase in their antioxidant capacity from small green to black ripe fruit. FRAP and TEAC assays showed high correlations with total polyphenolic content (TPC) during ripening and high antioxidant capacity at all fruit stages, showing greater values in ripe fruit (FRAP: 24 ± 2 and 28 ± 3 μM FeSO4/gFW; TEAC: 18 ± 2 and 20 ± 1 Eq. Trolox/gFW for each season, respectively) than those observed in the blueberry (FRAP: 10 ± 2 and 19 ± 3 μM FeSO4/gFW; TEAC: 10 ± 2 and 17 ± 3). In addition, bioactive assays using ripe fruit extracts show presence of flavonol and anthocyanins, a high ORAC value (62,500 ± 7000 μmol/gDW) and a concentration-dependent vascular protection under high glucose conditions. The results obtained show that these endemic berry fruits have a promising potential as functional food. Copyright © 2015 Elsevier Ltd. All rights reserved.

  14. Effect of light quality on development of fruiting bodies of Panus fragilis

    Science.gov (United States)

    Orson K. Miller; John G. Palmer

    1977-01-01

    Under a system that permits mass screening of mycelia within bands of the visible spectrum, fruit bodies initiated and developed in two light bands (387-400nm and 425-430nm) in axenic culture. Either or both of these light bands will trigger fruitbody initiation at as low an energy level as 0.2 K (1 K = 1,000 microwatts/cm2). Maturation of sporocarp and hymenium...

  15. Improved Stable Isotope Dilution Assay for Dietary Folates Using LC-MS/MS and Its Application to Strawberries

    Directory of Open Access Journals (Sweden)

    Lisa Striegel

    2018-02-01

    Full Text Available Folates play an important role in the human body and a deficiency of this vitamin can cause several diseases. Therefore, a reliable analytical method is crucial for the determination of folate vitamers in strawberries and other dietary folate sources. A stable isotope dilution LC-MS/MS method for analyzing folates in food was developed and validated. The folate vitamers Pteroylmonoglutamic acid, tetrahydrofolate, 5-methyltetrahydrofolate, and 5-formyltetrahydrofolate were quantified using 13C-labeled internal standards. Validation of the assay was accomplished by determining linearity, precision, recovery, limit of detection, and limit of quantification and revealed to be a precise, sensitive, and accurate method to determine folate vitamers. Strawberries are worldwide consumed and known to be a good dietary source of nutritive compounds. Using this method, folate concentrations in selected commercial strawberry cultivars and experimental breeding lines grown in Germany and Australia were investigated. Total folates varied from 59 to 153 μg/100 g on fresh weight basis. Furthermore, folate content after lyophilizing or freezing did not show any significant differences compared to fresh strawberries. However, significant losses of total folates in pureed strawberries could be observed after 5 days of storage with only 16% of the original concentration retained. In summary, some of the investigated strawberry cultivars/breeding lines can be considered as rich dietary sources of natural folates.

  16. Improved stable isotope dilution assay for dietary folates using LC-MS/MS and its application to strawberries

    Science.gov (United States)

    Striegel, Lisa; Chebib, Soraya; Netzel, Michael E.; Rychlik, Michael

    2018-02-01

    Folates play an important role in the human body and a deficiency of this vitamin can cause several diseases. Therefore, a reliable analytical method is crucial for the determination of folate vitamers in strawberries and other dietary folate sources. A stable isotope dilution LC-MS/MS method for analyzing folates in food was developed and validated. The folate vitamers Pteroylmonoglutamic acid, tetrahydrofolate, 5-methyltetrahydrofolate and 5-formyltetrahydrofolate were quantified using 13C-labelled internal standards. Validation of the assay was accomplished by determining linearity, precision, recovery, limit of detection and limit of quantification and revealed to be a precise, sensitive and accurate method to determine folate vitamers. Strawberries are worldwide consumed and known to be a good dietary source of nutritive compounds. Using this method, folate concentrations in selected commercial strawberry cultivars and experimental breeding lines grown in Germany were investigated. Total folates varied from 59 to 153 µg/100 g on fresh weight basis. Furthermore, folate content after lyophilizing or freezing did not show any significant differences compared to fresh strawberries. However, significant losses of total folates in pureed strawberries could be observed after 5 days of storage with only 16 % of the original concentration retained. In summary, some of the investigated strawberry cultivars/breeding lines can be considered as rich dietary sources of natural folates.

  17. Profiling and Quantification of Regioisomeric Caffeoyl Glucoses in Berry Fruits.

    Science.gov (United States)

    Patras, Maria A; Jaiswal, Rakesh; McDougall, Gordon J; Kuhnert, Nikolai

    2018-02-07

    On the basis of a recently developed tandem mass spectrometry-based hierarchical scheme for the identification of regioisomeric caffeoyl glucoses, selected berry fruits were profiled for their caffeoyl glucose ester content. Fresh edible berries profiled, including strawberries, raspberries, blueberries, blackberries, red currant, black currant, lingonberries, gooseberries, and juices of elderberries, goji berries, chokeberries, cranberries, açai berries, sea buckthorn berries, Montmorency sour cherries, and pomegranates, were investigated. 1-Caffeoyl glucose was found to be the predominant isomer in the majority of samples, with further profiling revealing the presence of additional hydroxycinnamoyl glucose esters and O-glycosides with p-coumaroyl, feruloyl, and sinapoyl substituents. A quantitative liquid chromatography-mass spectrometry-based method was developed and validated, and all caffeoyl glucose isomers were quantified for the first time in edible berries.

  18. Carbon partitioning among leaves, fruits, and seeds during development of Phaseolus vulgaris L

    International Nuclear Information System (INIS)

    Geiger, D.R.; Shieh, Wenjang; Saluke, R.M.

    1989-01-01

    Development of vegetative and floral buds was found to be a key factor in establishing the way carbon is distributed among growing leaves and fruits in Phaseolus vulgaris L. plants. Leaves emerged principally during a period 14 to 32 days after planting while flowers were produced during a 10- to 12-day period near the end of leaf emergence. Timing of anthesis established the sigmoidal time course for dry weight accumulated by the composite of all fruits on the plant. During the first 12 days following anthesis, fruit growth mainly consisted of elongation and dry weight accumulation by the pod wall. Thereafter, seed dry weight increased for about 1 week, decreased markedly for several days, and then increased again over the next 2 weeks. Accumulation of imported carbon in individual seeds, measured by steady-state labeling, confirmed the time course for dry weight accumulation observed during seed development. Seed respiration rate initially increased rapidly along with dry weight and then remained nearly steady until seed maturation. A number of developmental events described in the literature coincided with the different phases of diauxic growth. The results demonstrated the feasibility of relating current rates of carbon import in individual seeds measured with tracer 14 C to the rates of conversion of imported sucrose and use of the products for specific developmental processes. The resulting data are useful for evaluating the roles of conversion and utilization of imported sucrose in regulating import by developing seeds

  19. Validation of a pulsed electric field process to pasteurize strawberry puree

    Science.gov (United States)

    An inexpensive data acquisition method was developed to validate the exact number and shape of the pulses applied during pulsed electric fields (PEF) processing. The novel validation method was evaluated in conjunction with developing a pasteurization PEF process for strawberry puree. Both buffered...

  20. Identification, Isolation, and Expression Analysis of Heat Shock Transcription Factors in the Diploid Woodland Strawberry Fragaria vesca

    Directory of Open Access Journals (Sweden)

    Yang eHu

    2015-09-01

    Full Text Available Heat shock transcription factors (Hsfs are known to play dominant roles in plant responses to heat, as well as other abiotic or biotic stress stimuli. While the strawberry is an economically important fruit plant, little is known about the Hsf family in the strawberry. To explore the functions of strawberry Hsfs in abiotic and biotic stress responses, this study identified 17 Hsf genes (FvHsfs in a wild diploid woodland strawberry (Fragaria vesca, 2n = 2x = 14 and isolated 14 of these genes. Phylogenetic analysis divided the strawberry FvHsfs genes into three main groups. The evolutionary and structural analyses revealed that the FvHsf family is conserved. The promoter sequences of the FvHsf genes contain upstream regulatory elements corresponding to different stress stimuli. In addition, 14 FvHsf-GFP fusion proteins showed differential subcellular localization in Arabidopsis mesophyll protoplasts. Furthermore, we examined the expression of the 17 FvHsf genes in wild diploid woodland strawberries under various conditions, including abiotic stresses (heat, cold, drought, and salt, biotic stress (powdery mildew infection, and hormone treatments (abscisic acid, ethephon, methyl jasmonate, and salicylic acid. Fifteen of the 17 FvHsf genes exhibited distinct changes on the transcriptional level during heat treatment. Of these 15 FvHsfs, 8 FvHsfs also exhibited distinct responses to other stimuli on the transcriptional level, indicating versatile roles in the response to abiotic and biotic stresses. Taken together, the present work may provide the basis for further studies to dissect FvHsf function in response to stress stimuli.

  1. Quality of fresh-cut strawberry

    OpenAIRE

    Costa,Franciscleudo B; Duarte,Priscila S; Puschmann,Rolf; Finger,Fernando L

    2011-01-01

    The aim of this work was to study the physical, chemical, physiological and microbiological changes during the flow chart of fresh-cut strawberry. Strawberry cvs. Camarosa, Dover and Tudla, derived from experimental area of the Universidade Federal de Viçosa, were selected by color (red ¾) and absence of wound. Afterwards, the minimal processing was evaluated, as follows: fast cooling, water and ice, removal of the calyx followed by conservation at 5±0.5°C and 90-95% RH for 13 days, sanitatio...

  2. Dynamic Labeling Reveals Temporal Changes in Carbon Re-Allocation within the Central Metabolism of Developing Apple Fruit

    Directory of Open Access Journals (Sweden)

    Wasiye F. Beshir

    2017-10-01

    Full Text Available In recent years, the application of isotopically labeled substrates has received extensive attention in plant physiology. Measuring the propagation of the label through metabolic networks may provide information on carbon allocation in sink fruit during fruit development. In this research, gas chromatography coupled to mass spectrometry based metabolite profiling was used to characterize the changing metabolic pool sizes in developing apple fruit at five growth stages (30, 58, 93, 121, and 149 days after full bloom using 13C-isotope feeding experiments on hypanthium tissue discs. Following the feeding of [U-13C]glucose, the 13C-label was incorporated into the various metabolites to different degrees depending on incubation time, metabolic pathway activity, and growth stage. Evidence is presented that early in fruit development the utilization of the imported sugars was faster than in later developmental stages, likely to supply the energy and carbon skeletons required for cell division and fruit growth. The declined 13C-incorporation into various metabolites during growth and maturation can be associated with the reduced metabolic activity, as mirrored by the respiratory rate. Moreover, the concentration of fructose and sucrose increased during fruit development, whereas concentrations of most amino and organic acids and polyphenols declined. In general, this study showed that the imported compounds play a central role not only in carbohydrate metabolism, but also in the biosynthesis of amino acid and related protein synthesis and secondary metabolites at the early stage of fruit development.

  3. Cell wall structures leading to cultivar differences in softening rates develop early during apple (Malus x domestica) fruit growth.

    Science.gov (United States)

    Ng, Jovyn K T; Schröder, Roswitha; Sutherland, Paul W; Hallett, Ian C; Hall, Miriam I; Prakash, Roneel; Smith, Bronwen G; Melton, Laurence D; Johnston, Jason W

    2013-11-19

    There is a paucity of information regarding development of fruit tissue microstructure and changes in the cell walls during fruit growth, and how these developmental processes differ between cultivars with contrasting softening behaviour. In this study we compare two apple cultivars that show different softening rates during fruit development and ripening. We investigate whether these different softening behaviours manifest themselves late during ethylene-induced softening in the ripening phase, or early during fruit expansion and maturation. 'Scifresh' (slow softening) and 'Royal Gala' (rapid softening) apples show differences in cortical microstructure and cell adhesion as early as the cell expansion phase. 'Scifresh' apples showed reduced loss of firmness and greater dry matter accumulation compared with 'Royal Gala' during early fruit development, suggesting differences in resource allocation that influence tissue structural properties. Tricellular junctions in 'Scifresh' were rich in highly-esterified pectin, contributing to stronger cell adhesion and an increased resistance to the development of large airspaces during cell expansion. Consequently, mature fruit of 'Scifresh' showed larger, more angular shaped cells than 'Royal Gala', with less airspaces and denser tissue. Stronger cell adhesion in ripe 'Scifresh' resulted in tissue fracture by cell rupture rather than by cell-to-cell-separation as seen in 'Royal Gala'. CDTA-soluble pectin differed in both cultivars during development, implicating its involvement in cell adhesion. Low pectin methylesterase activity during early stages of fruit development coupled with the lack of immuno-detectable PG was associated with increased cell adhesion in 'Scifresh'. Our results indicate that cell wall structures leading to differences in softening rates of apple fruit develop early during fruit growth and well before the induction of the ripening process.

  4. Development of Export Performance Scale for Fresh Vegetable-Fruit Sector

    Directory of Open Access Journals (Sweden)

    Murat Keskinkılınç

    2018-06-01

    Full Text Available The purpose of this paper is to propose a scale for assessing the performance of foreign trade companies in fresh vegetable-fruit sector. As a first step, a qualitative interviews was conducted to the sample consists of the managers working in export companies. As a result of the interviews major problems of exporters were grouped. In the second phase of the study a questionnaire was formed and a survey was conducted to the larger sample. Subsequently, validity and reliability of the scales were determined by means of explanatory and confirmatory factor analyses and reliability analysis respectively. The theoretical contribution of this research is the development of a method for evaluation of export performance of foreign trade companies in fresh vegetable-fruit sector.

  5. Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea.

    Directory of Open Access Journals (Sweden)

    Hajime Muraguchi

    Full Text Available The basidiomycete fungus Coprinopsis cinerea is an important model system for multicellular development. Fruiting bodies of C. cinerea are typical mushrooms, which can be produced synchronously on defined media in the laboratory. To investigate the transcriptome in detail during fruiting body development, high-throughput sequencing (RNA-seq was performed using cDNA libraries strand-specifically constructed from 13 points (stages/tissues with two biological replicates. The reads were aligned to 14,245 predicted transcripts, and counted for forward and reverse transcripts. Differentially expressed genes (DEGs between two adjacent points and between vegetative mycelium and each point were detected by Tag Count Comparison (TCC. To validate RNA-seq data, expression levels of selected genes were compared using RPKM values in RNA-seq data and qRT-PCR data, and DEGs detected in microarray data were examined in MA plots of RNA-seq data by TCC. We discuss events deduced from GO analysis of DEGs. In addition, we uncovered both transcription factor candidates and antisense transcripts that are likely to be involved in developmental regulation for fruiting.

  6. Transcriptome analysis of a spontaneous mutant in sweet orange [Citrus sinensis (L.) Osbeck] during fruit development.

    Science.gov (United States)

    Liu, Qing; Zhu, Andan; Chai, Lijun; Zhou, Wenjing; Yu, Keqin; Ding, Jian; Xu, Juan; Deng, Xiuxin

    2009-01-01

    Bud mutations often arise in citrus. The selection of mutants is one of the most important breeding channels in citrus. However, the molecular basis of bud mutation has rarely been studied. To identify differentially expressed genes in a spontaneous sweet orange [C. sinensis (L.) Osbeck] bud mutation which causes lycopene accumulation, low citric acid, and high sucrose in fruit, suppression subtractive hybridization and microarray analysis were performed to decipher this bud mutation during fruit development. After sequencing of the differentially expressed clones, a total of 267 non-redundant transcripts were obtained and 182 (68.2%) of them shared homology (E-value or = 2) in the bud mutation during fruit development. Self-organizing tree algorithm analysis results showed that 95.1% of the differentially expressed genes were extensively coordinated with the initiation of lycopene accumulation. Metabolic process, cellular process, establishment of localization, response to stimulus, and biological regulation-related transcripts were among the most regulated genes. These genes were involved in many biological processes such as organic acid metabolism, lipid metabolism, transport, and pyruvate metabolism, etc. Moreover, 13 genes which were differentially regulated at 170 d after flowering shared homology with previously described signal transduction or transcription factors. The information generated in this study provides new clues to aid in the understanding of bud mutation in citrus.

  7. Exposure to environmentally-relevant levels of ozone negatively influence pollen and fruit development.

    Science.gov (United States)

    Gillespie, Colin; Stabler, Daniel; Tallentire, Eva; Goumenaki, Eleni; Barnes, Jeremy

    2015-11-01

    A combination of in vitro and in vivo studies on tomato (Lycopersicon esculentum Mill. cv. Triton) revealed that environmentally-relevant levels of ozone (O3) pollution adversely affected pollen germination, germ tube growth and pollen-stigma interactions - pollen originating from plants raised in charcoal-Purafil(®) filtered air (CFA) exhibited reduced germ tube development on the stigma of plants exposed to environmentally-relevant levels of O3. The O3-induced decline in in vivo pollen viability was reflected in increased numbers of non-fertilized and fertilized non-viable ovules in immature fruit. Negative effects of O3 on fertilization occurred regardless of the timing of exposure, with reductions in ovule viability evident in O3 × CFA and CFA × O3 crossed plants. This suggests O3-induced reductions in fertilization were associated with reduced pollen viability and/or ovule development. Fruit born on trusses independently exposed to 100 nmol mol(-1) O3 (10 h d(-1)) from flowering exhibited a decline in seed number and this was reflected in a marked decline in the weight and size of individual fruit - a clear demonstration of the direct consequence of the effects of the pollutant on reproductive processes. Ozone exposure also resulted in shifts in the starch and ascorbic acid (Vitamin C) content of fruit that were consistent with accelerated ripening. The findings of this study draw attention to the need for greater consideration of, and possibly the adoption of weightings for the direct impacts of O3, and potentially other gaseous pollutants, on reproductive biology during 'risk assessment' exercises. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Radiation induced chemical changes of phenolic compounds in strawberries

    International Nuclear Information System (INIS)

    Breitfellner, F.; Solar, S.; Sontag, G.

    2002-01-01

    Complete text of publication follows. The investigations were directed to the determination of the effect of γ-irradiation on various phenolic compounds in strawberries in dependence of dose. A significant decrease of these compounds during irradiation could reduce their beneficial effect on health, which are based on their antioxidative and anticarcinogenic properties. On the other hand hydroxilation of phenolic acids has been proposed as a promising method to distinguish between irradiated and not irradiated fruits and vegetables. Irradiated and not irradiated strawberry samples were homogenized, centrifuged and chromatographically purified from matrix components on polyamide columns. For determination of hydroxybenzoic and hydroxycinnamic acids, which are present as esters or as glycosides, the samples had to be acid/base hydrolized prior to purification. The individual compounds were separated by reversed phase chromatography and detected by means of a diode-array-detector. Peak identification was based on both UV-Vis-spectra and retention times compared with those of standards. In hydrolized samples four phenolic acids [gallic acid, 4-hydroxybenzoic acid, p-coumaric acid and caffeic acid] were identified. Only 4-hydroxybenzoic acid was affected by irradiation (build up with dose). Five flavonoids were detected in non hydrolized samples [(+)-catechin, (-)-epicatechin, kaempferol-3-glucoside, quercetin-3-glucoside and, in trace quantities, quercetin-3-galactoside], the concentration of the catechines and of kaempferol-3-glucoside decreased as irradiation dose increased, whereas those of quercetin-3-glucoside remained unchanged. In addition two as yet unclassified compounds showed a significant change of concentration upon irradiation. One of them (m/e = 450) is decreasing, one (m/e = 318) is increasing to the fivefold at a dose of 6 kGy

  9. Acai fruit improves motor and cognitive function in aged rats

    Science.gov (United States)

    Aged rats show impaired performance on motor and cognitive tasks that require the use of spatial learning and memory. In previous studies, we have shown the beneficial effects of various berry fruits (blueberries, strawberries, and blackberries) in reversing age-related deficits in behavioral and ne...

  10. Chilean Strawberry Consumption Protects against LPS-Induced Liver Injury by Anti-Inflammatory and Antioxidant Capability in Sprague-Dawley Rats

    OpenAIRE

    Molinett, Sebastian; Nuñez, Francisca; Moya-León, María Alejandra; Zúñiga-Hernández, Jessica

    2015-01-01

    The Chilean strawberry fruit has high content of antioxidants and polyphenols. Previous studies evidenced antioxidant properties by in vitro methods. However, the antioxidant effect and its impact as functional food on animal health have not been evaluated. In this study, rats were fed with a Chilean strawberry aqueous extract (4 g/kg of animal per day) and then subjected to LPS-induced liver injury (5 mg/kg). Transaminases and histological studies revealed a reduction in liver injury in rats...

  11. Effect of gamma radiation on the growth of botryodiplodia theobromae and on rot development in banana fruits

    International Nuclear Information System (INIS)

    Mostafa, I.Y.; El-Ashmawi, A.M.; Fahim, M.M.; Kararah, M.A.

    1984-01-01

    Radiosensitivity of Botryodiplodia theobromae Pat. increased with the increase in doses of gamma radiation. Young cultures (7 days old) were less sensitive to radiation than old ones (48 days); lethal doses being 800 and 600 Krad respectively. Rot development was greatly reduced when inoculated banana fruits were exposed to 300 Krad. Disease development was checked for 10 days in inoculated fruits exposed to 400 Krad. The combined treatment of 200 p.p.m. T B Z and 100 or 200 Krad gamma radiation was more effective in reducing disease incidence than either treatments alone. No deleterious effects occurred in banana fruits that were irradiated with the low doses of gamma radiation

  12. Survey of quality indicators in commercial dehydrated fruits.

    Science.gov (United States)

    Megías-Pérez, Roberto; Gamboa-Santos, Juliana; Soria, Ana Cristina; Villamiel, Mar; Montilla, Antonia

    2014-05-01

    Physical and chemical quality parameters (dry matter, aw, protein, carbohydrates, vitamin C, 2-furoylmethyl amino acids, rehydration ratio and leaching loss) have been determined in 30 commercial dehydrated fruits (strawberry, blueberry, raspberry, cranberry, cherry, apple, grapefruit, mango, kiwifruit, pineapple, melon, coconut, banana and papaya). For comparison purposes, strawberry samples processed in the laboratory by freeze-drying and by convective drying were used as control samples. Overall quality of dehydrated fruits seemed to be greatly dependent on processing conditions and, in a cluster analysis, samples which were presumably subjected to osmotic dehydration were separated from the rest of fruits. These samples presented the lowest concentration of vitamin C and the highest evolution of Maillard reaction, as evidenced by its high concentration of 2-furoylmethyl amino acids. This is the first study on the usefulness of this combination of chemical and physical indicators to assess the overall quality of commercial dehydrated fruits. Copyright © 2013 Elsevier Ltd. All rights reserved.

  13. Microsatellite marker development by partial sequencing of the sour passion fruit genome (Passiflora edulis Sims).

    Science.gov (United States)

    Araya, Susan; Martins, Alexandre M; Junqueira, Nilton T V; Costa, Ana Maria; Faleiro, Fábio G; Ferreira, Márcio E

    2017-07-21

    The Passiflora genus comprises hundreds of wild and cultivated species of passion fruit used for food, industrial, ornamental and medicinal purposes. Efforts to develop genomic tools for genetic analysis of P. edulis, the most important commercial Passiflora species, are still incipient. In spite of many recognized applications of microsatellite markers in genetics and breeding, their availability for passion fruit research remains restricted. Microsatellite markers in P. edulis are usually limited in number, show reduced polymorphism, and are mostly based on compound or imperfect repeats. Furthermore, they are confined to only a few Passiflora species. We describe the use of NGS technology to partially assemble the P. edulis genome in order to develop hundreds of new microsatellite markers. A total of 14.11 Gbp of Illumina paired-end sequence reads were analyzed to detect simple sequence repeat sites in the sour passion fruit genome. A sample of 1300 contigs containing perfect repeat microsatellite sequences was selected for PCR primer development. Panels of di- and tri-nucleotide repeat markers were then tested in P. edulis germplasm accessions for validation. DNA polymorphism was detected in 74% of the markers (PIC = 0.16 to 0.77; number of alleles/locus = 2 to 7). A core panel of highly polymorphic markers (PIC = 0.46 to 0.77) was used to cross-amplify PCR products in 79 species of Passiflora (including P. edulis), belonging to four subgenera (Astrophea, Decaloba, Distephana and Passiflora). Approximately 71% of the marker/species combinations resulted in positive amplicons in all species tested. DNA polymorphism was detected in germplasm accessions of six closely related Passiflora species (P. edulis, P. alata, P. maliformis, P. nitida, P. quadrangularis and P. setacea) and the data used for accession discrimination and species assignment. A database of P. edulis DNA sequences obtained by NGS technology was examined to identify microsatellite repeats in

  14. Development and chemical characterization of flour obtained from the external mesocarp of "pequizeiro" fruit

    Directory of Open Access Journals (Sweden)

    Manoel Soares Soares Júnior

    2010-12-01

    Full Text Available The objective of this work was to develop a recommendation for the chemical peeling of pequi fruit and characterize the flour obtained from the external mesocarp of "Pequizeiro", pequi tree (Caryocar brasiliense Camb.. The technology applied to obtain the external mesocarp pequi flour included the epicarp removal with NaOH solution. The Response Surface Method was used to optimize the chemical peeling process by applying the Central Composite Rotatable Design, with eleven trials including three replicates at the central point, varying the NaOH aqueous solution concentration and fruit immersion time. The mass loss was evaluated through the analysis of variance and using bi and three dimensional graphs. The chemical characteristics of the external mesocarp pequi flour evaluated were: moisture content, ashes, proteins, lipids, total carbohydrates, dietary fiber, and some minerals. The best combination for an efficient removal of the fruit peel with the lowest mass loss was reached with 7.05 minutes of immersion in a 5.08 g.L-1 NaOH aqueous solution. This study indicated that the external mesocarp pequi flour is a food source rich in dietary fiber, carbohydrates, ashes, magnesium, calcium, manganese, and copper, but it is poor in lipids, zinc, and iron.

  15. Changes In water translocation in the vascular tissue of grape during fruit development

    International Nuclear Information System (INIS)

    Zhaosen, X.; Forney, C.F.

    2014-01-01

    The relationship between vascular water translocation in grapes and berry growth was investigated. Berry growth, firmness and turgor were measured, and the structure and function of the vascular bundles for water translocation was observed. During phase I fruit development, the dorsal and central vascular bundles rapidly translocated introduced dye in the pedicle. The speed of dye translocation was highest in the dorsal vascular bundles of phase I fruit with a speed of 0.97cm/h. After phase II, both the distribution of dye and the speed of dye translocation in the fruit vascular tissue decreased, with speeds in the dorsal and central vascular bundles being 0.08 cm/h and 0.72 cm/h, respectively. During phase III, the distribution of dye was still lower than phase I. After phase II, the walls of some xylem vessels were indistinct and broken. After phase III, even though the water translocation efficiency of the xylem decreased, sugar accumulation in the berry as well as osmoregulation increased. (author)

  16. Development and Characterization of Edible Films Based on Fruit and Vegetable Residues.

    Science.gov (United States)

    Andrade, Roberta M S; Ferreira, Mariana S L; Gonçalves, Édira C B A

    2016-02-01

    Edible films were developed from the solid residue of the processing of whole fruits and vegetables. The solid residue, processed into flour (FVR flour) was chemically and structurally characterized by microstructure, elemental composition, structural links, and moisture sorption isotherm. Films were prepared by casting using aqueous extracts of 8% and 10% of flour (w/w) and characterized in terms of thickness, water solubility, mechanical properties, water vapor permeability, and Fourier transform infrared (FTIR). The analysis of microstructure and elemental composition, performed on flour (mean particle size 350 μm), showed an essentially granular aspect, with the presence of fibrous particles having potassium as one of the most abundant elements. FTIR results showed similarity between the characteristic bands of other raw materials used in edible films. The sorption isotherm of FVR flour showed a typical profile of foods rich in soluble components, such as sugars. Dried films presented an average thickness of 0.263 ± 0.003 mm, a homogenous aspect, bright yellow color, pronounced fruit flavor, and high water solubility. The FTIR spectra of the edible films revealed that addition of potato skin flour did not change the molecular conformation. Moreover, the films presented low tensile strength at break when compared with fruit starch-based films. © 2016 Institute of Food Technologists®

  17. Combined heat and gamma-irradiation treatments for the control of strawberry diseases under market conditions

    International Nuclear Information System (INIS)

    Brodrick, H.T.; Thomas, A.C.; Van Tonder, A.J.; Terblanche, J.C.

    1977-02-01

    The spoilage of strawberries under local market conditions was investigated. It was confirmed that the major losses are due to 'leak' disease caused by Rhizopus stolonifer (Ehr. ex Fr.) Lind. It was also established that further fruit losses in summer are due to anthracnose caused by the fungus Colletotrichum acutatum Simmonds. This is the first time that the latter pathogen has been isolated and identified and recognised as a problem on strawberries in South Africa. Studies with R. stolonifer in culture showed that 46 degrees Celsius for 20 min (the previous international standard heat treatment for fruit) was disappointing, while a treatment at 50 degrees Celsius for 10 min effectively inhibited spore germination. Irradiation studies with cultures of R. stolonifer and C. acutatum showed that a dose of 200 and 100 krad, respectively, resulted in excellent inhibition of spore germination. However, irradiating in nitrogen gas resulted in a tenfold reduction in the effectiveness of the irradiation treatments. The use of nitrogen during irradiation, therefore, cannot be considered, especially where an effective control of the fungal pathogens is desired. Investigations with different cultivars clearly demonstrated the synergistic effect on disease control obtained when combining heat and irradiation treatments. The combination treatment (moist heat at 50-52 degrees Celsius for 10 min plus 200 krad), besides effectively controlling both diseases in strawberries, did not adversely affect berry quality. In simulated transport tests it was shown that a minimal amount of berry softening did occur with this treatment, but this adverse effect was negligible compared with the beneficial effect obtained from disease control. In semi-commercial experiments it was shown that the combination heat and irradiation treatment effectively controlled spoilage diseases for a period of several days from picking, thus allowing sufficient time to market the fruit under local market

  18. Electron spin resonance identification of irradiated fruits

    International Nuclear Information System (INIS)

    Raffi, J.J.; Agnel, J.-P.L.

    1989-01-01

    The electron spin resonance spectrum of achenes, pips, stalks and stones from irradiated fruits (stawberry, raspberry, red currant, bilberry, apple, pear, fig, french prune, kiwi, water-melon and cherry) always displays, just after γ-treatment, a weak triplet (a H ∼30 G) due to a cellulose radical; its left line (lower field) can be used as an identification test of irradiation, at least for strawberries, raspberries, red currants or bilberries irradiated in order to improve their storage time. (author)

  19. Production of strawberry cultivars in closed hydroponic systems and coconut fibre substrate

    Directory of Open Access Journals (Sweden)

    Fabio Rodrigues de Miranda

    Full Text Available The objective of this work was to evaluate yield and average fruit weight of strawberry cultivars Albion, Camarosa, Festival and Oso Grande, in two closed hydroponic systems (gutters and grow bags, using coconut fibre as substrate. The experimental design was of randomised blocks, divided into strips, with five replications. The hydroponic systems did not differ significantly as to yield, with advantages, such as savings in water and fertilizer and reduced environmental impact, over open systems. The most productive cultivar was Festival, followed by Oso Grande, with average yields of 6.99 kg m-2 and 5.56 kg m-2 respectively. The cultivars with the greatest fruit weight were Oso Grande and Albion, having averages of 11.8 and 11.1 g respectively, with the former being significantly superior to the latter. The highest yield (7.4 kg m-2 was obtained from the cultivar Festival under the gutter system. The Ibiapaba region has conditions which are favourable to strawberry production in relation to precocity (harvesting starts in the 6th week of growth and production continues throughout the year; however there is a need to test new cultivars and to improve the cultivation techniques with an aim to producing larger-sized fruit.

  20. Foliar uptake of 134Cs and 85Sr in strawberry as function by leaf age

    International Nuclear Information System (INIS)

    Fortunati, P.; Brambilla, M.; Speroni, F.; Carini, F.

    2004-01-01

    In this paper a study of the foliar uptake and translocation of 134 Cs and 85 Sr in a herbaceous fruit plant is presented. In particular, absorption, translocation and loss of these radionuclides in strawberry plants have been studied in relation to the age of contaminated leaves. Strawberry plants were contaminated by distributing droplets of an aqueous solution containing 134 CsCl and 85 SrCl 2 on the surface of two leaves per plant. One half of the plants was contaminated through two young leaves, a second half through two old leaves. Sets of plants were collected 1 day, 7 days and 15 days after contamination. One half of them was rinsed with double distilled water before gamma analysis. Rinsing contaminated leaves removes on average 55% of the applied 134 Cs and 45% of 85 Sr. The activity removed decreases during the 15 days of the experimental study, both for 134 Cs and for 85 Sr, suggesting an increase in foliar absorption during this period. The activity removed does not differ between old and young leaves. 'External loss' is lower for young than old contaminated leaves. 'Internal loss' through translocation occurs mainly for 134 Cs. Translocation coefficients from contaminated leaves to fruits are two orders of magnitude higher for 134 Cs (4.0%), than for 85 Sr (0.05%). Leaf to fruit translocation coefficients for 134 Cs are higher from young leaves (5.8%), than from old leaves (2.3%)

  1. Sensation™ ‘Florida 127’ Strawberry

    Science.gov (United States)

    ‘Florida127’ strawberry originated from a 2009 cross between Winterstar™ ‘FL 05-107’ (female parent) and unreleased selection FL 02-58 (male parent). It is a short-day genotype adapted to an annual plasticulture growing system. The plant is upright with open architecture, allowing air movement and e...

  2. Influence of NaCl salinity on growth analysis of strawberry cv. Camarosa

    Directory of Open Access Journals (Sweden)

    H. Mirdehghan

    2011-12-01

    Full Text Available In order to study of salinity effect on growth analysis of strawberry, a greenhouse experiment was conducted in Vali-e-Asr University of Rafsanjan in 2010. This study was carried out RCBD design with 4 replications to determine the influence of salinity (30, 60, 90 Mmol and control with distilled water on strawberry growth analysis. Results indicated that relative growth rate (RGR, crop growth rate (CGR, leaf area ratio (LAR and dry matter accumulation were decreased with increasing salinity. The lowest RGR, CGR and LAR were observed in 90 Mmol NaCl salinity. Results also indicated that maximum dry matter accumulations were observed in 1050, 1200 and 1400 degree days in 30, 60 and 90 Mmol NaCl salinity, respectively. Water salinity more than 30 Mmol NaCl L-1 will decreased fresh fruit yield more than 50 percent in hydroponics strawberry production. Dry mass partitioning in NaCl-stressed plants was in favor of crown and petioles and at expense of root, stem and leaf whereas leaf, stem and root DM progressively declined with an increase in salinity.

  3. Abiotic Stress-Related Expressed Sequence Tags from the Diploid Strawberry Fragaria vesca f. semperflorens

    Directory of Open Access Journals (Sweden)

    Maximo. Rivarola

    2011-03-01

    Full Text Available Strawberry ( spp. is a eudicotyledonous plant that belongs to the Rosaceae family, which includes other agronomically important plants such as raspberry ( L. and several tree-fruit species. Despite the vital role played by cultivated strawberry in agriculture, few stress-related gene expression characterizations of this crop are available. To increase the diversity of available transcriptome sequence, we produced 41,430 L. expressed sequence tags (ESTs from plants growing under water-, temperature-, and osmotic-stress conditions as well as a combination of heat and osmotic stresses that is often found in irrigated fields. Clustering and assembling of the ESTs resulted in a total of 11,836 contigs and singletons that were annotated using Gene Ontology (GO terms. Furthermore, over 1200 sequences with no match to available Rosaceae ESTs were found, including six that were assigned the “response to stress” GO category. Analysis of EST frequency provided an estimate of steady state transcript levels, with 91 sequences exhibiting at least a 20-fold difference between treatments. This EST collection represents a useful resource to advance our understanding of the abiotic stress-response mechanisms in strawberry. The sequence information may be translated to valuable tree crops in the Rosaceae family, where whole-plant treatments are not as simple or practical.

  4. Comparative genomic sequence analysis of strawberry and other rosids reveals significant microsynteny

    Directory of Open Access Journals (Sweden)

    Abbott Albert

    2010-06-01

    Full Text Available Abstract Background Fragaria belongs to the Rosaceae, an economically important family that includes a number of important fruit producing genera such as Malus and Prunus. Using genomic sequences from 50 Fragaria fosmids, we have examined the microsynteny between Fragaria and other plant models. Results In more than half of the strawberry fosmids, we found syntenic regions that are conserved in Populus, Vitis, Medicago and/or Arabidopsis with Populus containing the greatest number of syntenic regions with Fragaria. The longest syntenic region was between LG VIII of the poplar genome and the strawberry fosmid 72E18, where seven out of twelve predicted genes were collinear. We also observed an unexpectedly high level of conserved synteny between Fragaria (rosid I and Vitis (basal rosid. One of the strawberry fosmids, 34E24, contained a cluster of R gene analogs (RGAs with NBS and LRR domains. We detected clusters of RGAs with high sequence similarity to those in 34E24 in all the genomes compared. In the phylogenetic tree we have generated, all the NBS-LRR genes grouped together with Arabidopsis CNL-A type NBS-LRR genes. The Fragaria RGA grouped together with those of Vitis and Populus in the phylogenetic tree. Conclusions Our analysis shows considerable microsynteny between Fragaria and other plant genomes such as Populus, Medicago, Vitis, and Arabidopsis to a lesser degree. We also detected a cluster of NBS-LRR type genes that are conserved in all the genomes compared.

  5. CATT: a New and Non-Chemical Pest and Nematode Control Method in Strawberry Planting Stock

    NARCIS (Netherlands)

    Kruistum, van G.; Evenhuis, B.; Hoek, J.; Kastelein, P.; Wolf, van der J.M.; Verschoor, J.A.

    2014-01-01

    As an alternative to MeBr fumigation a 48h Controlled Atmosphere Temperature Treatment (CATT) was developed and scaled up by Wageningen UR in cooperation with the Dutch plant propagating association Plantum. This results in an excellent de-infestation and 99.8 % mortality of the strawberry

  6. Modelling formulae of strawberry whey drinks of prophylactic application

    Directory of Open Access Journals (Sweden)

    N. Tkachenko

    2017-04-01

    Full Text Available Expediency of the development of formulae and innovative technologies for production of prophylactic application drinks possessing antioxidant, probiotic and hepatoprotective properties with the use of the secondary dairy product – whey, as well as the domestic vegetable raw materials having a high content of bioactive substances has been substantiated.Formulation composition of the prophylactic drinks based on cheese whey, extract of Tagetes patula flowers and the berry filler “Strawberry” with the use of the response surface method has been developed. Bioactivity of the drinks and the complex quality indicator which accounts for the total influence of the bioactivity, organoleptic assessment and weight coefficients of the specified unit indicators were taken as the optimization criteria; as the independent factors that were varied in the course of the experiment, the mass fractions of the marigold flowers extract and the strawberries filler were selected. It is recommended that the mass fractions of the berry filler “Strawberry” and the extract of Tagetes patula flowers in the prophylactic drinks are set as 7 and 20 % of the finished product, accordingly. The practical mass fraction of the citric acid of 0.2 % was determined as it ensures high organoleptic characteristics of the finished drinks. Recommendations are given concerning development of innovative technologies of unfermented and fermented strawberry whey drinks of prophylactic application enriched with the extract of Tagetes patula flowers.

  7. Effect of a gelatin-based edible coating containing cellulose nanocrystals (CNC) on the quality and nutrient retention of fresh strawberries during storage

    Science.gov (United States)

    Fakhouri, F. M.; Casari, A. C. A.; Mariano, M.; Yamashita, F.; Innocnentini Mei, L. H.; Soldi, V.; Martelli, S. M.

    2014-08-01

    Strawberry is a non-climacteric fruit with a very short postharvest shelf-life. Loss of quality in this fruit is mostly due to its relatively high metabolic activity and sensitivity to fungal decay, meanly grey mold (Botrytis cinerea). In this study, the ability of gelatin coatings containing cellulose nanocrystals (CNC) to extend the shelf-life of strawberry fruit (Fragaria ananassa) over 8 days were studied. The filmogenic solution was obtained by the hydration of 5 g of gelatin (GEL) in 100 mL of distillated water containing different amounts of CNC dispersion (10 mg CNC/g of GEL or 50 mg of CNC/g of GEL) for 1 hour at room temperature. After this period, the solution was heated to 70 °C and maintained at this temperature for 10 minutes. The plasticizer (glycerol) (10g/100g of the GEL) was then added with constant, gentle stirring in order to avoid forming air bubbles and also to avoid gelatin denaturation until complete homogenization. Strawberries (purchased at the local market) were immersed in the filmogenic solution for 1 minute and after coated were dried at 15 °C by 24 hours. The strawberries were then kept under refrigeration and characterized in terms of their properties (weight loss, ascorbic acid content, titratable acidity, water content). The results have shown that samples covered with GEL/CNC had a significant improvement in its shelf- life. For instance, for the control sample (without coating) the weight loss after 8 days of storage was around 65%, while covered samples loss in the range of 31-36%. Edible coating was also effective in the retention of ascorbic acid (AA) in the strawberries, while control sample presented a fast decay in the AA content, covered samples showed a slow decay in the AA concentration. Moreover, the use of GEL/CNC edible coating had an antimicrobial effect in the fruits.

  8. Effect of a gelatin-based edible coating containing cellulose nanocrystals (CNC) on the quality and nutrient retention of fresh strawberries during storage

    International Nuclear Information System (INIS)

    Fakhouri, F M; Casari, A C A; Martelli, S M; Mariano, M; Soldi, V; Yamashita, F; Mei, L H Innocnentini

    2014-01-01

    Strawberry is a non-climacteric fruit with a very short postharvest shelf-life. Loss of quality in this fruit is mostly due to its relatively high metabolic activity and sensitivity to fungal decay, meanly grey mold (Botrytis cinerea). In this study, the ability of gelatin coatings containing cellulose nanocrystals (CNC) to extend the shelf-life of strawberry fruit (Fragaria ananassa) over 8 days were studied. The filmogenic solution was obtained by the hydration of 5 g of gelatin (GEL) in 100 mL of distillated water containing different amounts of CNC dispersion (10 mg CNC/g of GEL or 50 mg of CNC/g of GEL) for 1 hour at room temperature. After this period, the solution was heated to 70 °C and maintained at this temperature for 10 minutes. The plasticizer (glycerol) (10g/100g of the GEL) was then added with constant, gentle stirring in order to avoid forming air bubbles and also to avoid gelatin denaturation until complete homogenization. Strawberries (purchased at the local market) were immersed in the filmogenic solution for 1 minute and after coated were dried at 15 °C by 24 hours. The strawberries were then kept under refrigeration and characterized in terms of their properties (weight loss, ascorbic acid content, titratable acidity, water content). The results have shown that samples covered with GEL/CNC had a significant improvement in its shelf- life. For instance, for the control sample (without coating) the weight loss after 8 days of storage was around 65%, while covered samples loss in the range of 31-36%. Edible coating was also effective in the retention of ascorbic acid (AA) in the strawberries, while control sample presented a fast decay in the AA content, covered samples showed a slow decay in the AA concentration. Moreover, the use of GEL/CNC edible coating had an antimicrobial effect in the fruits

  9. Embryo sac development in yellow passion fruit Passiflora edulis f. flavicarpa (Passifloraceae

    Directory of Open Access Journals (Sweden)

    Margarete Magalhães de Souza

    2002-01-01

    Full Text Available The yellow passion fruit, Passiflora edulis f. flavicarpa, is one of the most important Brazilian fruit crops. It is an allogamous, diploid, and self-incompatible species. It has hermaphrodite, solitary flowers, located in the leaf axils and protected by leaf bracts. The flower has an androgynophore, which is a straight stalk supporting its reproductive parts. There are usually five anthers, located at the tip of each of the five filaments. The ovary is borne just above the filaments, at the top of the androgynophore; there are three styles that are united at their base, and at the top there are three stigmas. The objective of this research was to observe embryo sac development in yellow passion flowers. Ovaries at different stages of development were fixed in FAA (formalin, acetic acid and alcohol solution, hydrated, stained with Mayer’s hemalum, and dehydrated. Ovules were cleared by using methyl salicylate, mounted on slides, and observed through a confocal scanning laser microscope. The yellow passion fruit ovule is bitegmic, crassinucellate, and anatropous, and its gametophyte development is of the Polygonum type. After meiosis, functional megaspores under go three successive mitotic divisions, resulting in an eight-nucleate megagametophyte: the egg apparatus at the micropylar end, two polar nuclei at the cell center, and three antipodals at the chalazal end. The egg apparatus is formed by an egg cell and two synergids, each with a filiform apparatus. The mature embryo sac has an egg cell, two synergids, two polar nuclei, and three antipodes, as has been described for most angiosperms.

  10. Prunasin Hydrolases during Fruit Development in Sweet and Bitter Almonds1[C][W][OA

    Science.gov (United States)

    Sánchez-Pérez, Raquel; Belmonte, Fara Sáez; Borch, Jonas; Dicenta, Federico; Møller, Birger Lindberg; Jørgensen, Kirsten

    2012-01-01

    Amygdalin is a cyanogenic diglucoside and constitutes the bitter component in bitter almond (Prunus dulcis). Amygdalin concentration increases in the course of fruit formation. The monoglucoside prunasin is the precursor of amygdalin. Prunasin may be degraded to hydrogen cyanide, glucose, and benzaldehyde by the action of the β-glucosidase prunasin hydrolase (PH) and mandelonitirile lyase or be glucosylated to form amygdalin. The tissue and cellular localization of PHs was determined during fruit development in two sweet and two bitter almond cultivars using a specific antibody toward PHs. Confocal studies on sections of tegument, nucellus, endosperm, and embryo showed that the localization of the PH proteins is dependent on the stage of fruit development, shifting between apoplast and symplast in opposite patterns in sweet and bitter cultivars. Two different PH genes, Ph691 and Ph692, have been identified in a sweet and a bitter almond cultivar. Both cDNAs are 86% identical on the nucleotide level, and their encoded proteins are 79% identical to each other. In addition, Ph691 and Ph692 display 92% and 86% nucleotide identity to Ph1 from black cherry (Prunus serotina). Both proteins were predicted to contain an amino-terminal signal peptide, with the size of 26 amino acid residues for PH691 and 22 residues for PH692. The PH activity and the localization of the respective proteins in vivo differ between cultivars. This implies that there might be different concentrations of prunasin available in the seed for amygdalin synthesis and that these differences may determine whether the mature almond develops into bitter or sweet. PMID:22353576

  11. Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata

    Directory of Open Access Journals (Sweden)

    Wa Gao

    2016-02-01

    Full Text Available The commercial method for isolation of ampelopsin, one of the most common flavonoids isolated from the plant species Ampelopsis grossedentata, is a simple hydrothermal extraction at high temperature. To develop an improved process to isolate ampelopsin, the effects of treatment of cellulase on hydrolysis of the dried fruit of A. grossedentata were investigated. The treatment of cellulase was found to decrease the temperature and time for hydrolysis of the dried fruit of A. grossedentata. The conditions of the filter press and continuous flow centrifuge for removal of insoluble materials from the hydrolysate of the dried fruit of A. grossedentata were optimized. The recovery yield of ampelopsin from the dried fruits of A. grossedentata was 39.4%, as determined by HPLC chromatographic analysis. A safe and economical process at low temperature with treatment of cellulase for the isolation of ampelopsin was developed in this study.

  12. Mining secreted proteins that function in pepper fruit development and ripening using a yeast secretion trap (YST)

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Je Min, E-mail: jemin@knu.ac.kr [Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul (Korea, Republic of); Department of Horticultural Science, Kyungpook National University, Daegu (Korea, Republic of); Lee, Sang-Jik [Biotechnology Institute, Nongwoo Bio Co, Ltd, Yeoju (Korea, Republic of); Department of Plant Biology, Cornell University, Ithaca, NY (United States); Rose, Jocelyn K.C. [Department of Plant Biology, Cornell University, Ithaca, NY (United States); Yeam, Inhwa [Department of Horticulture and Breeding, Andong National University, Andong (Korea, Republic of); Kim, Byung-Dong [Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul (Korea, Republic of)

    2014-04-18

    Highlights: • Yeast secretion trap (YST) is a valuable tool for mining secretome. • A total of 80 secreted proteins are newly identified via YST in pepper fruits. • The secreted proteins are differentially regulated during pepper development and ripening. • Transient GFP-fusion assay and in planta secretion trap can effectively validate the secretion of proteins. - Abstract: Plant cells secrete diverse sets of constitutively- and conditionally-expressed proteins under various environmental and developmental states. Secreted protein populations, or secretomes have multiple functions, including defense responses, signaling, metabolic processes, and developmental regulation. To identify genes encoding secreted proteins that function in fruit development and ripening, a yeast secretion trap (YST) screen was employed using pepper (Capsicum annuum) fruit cDNAs. The YST screen revealed 80 pepper fruit-related genes (CaPFRs) encoding secreted proteins including cell wall proteins, several of which have not been previously described. Transient GFP-fusion assay and an in planta secretion trap were used to validate the secretion of proteins encoded by selected YST clones. In addition, RNA gel blot analyses provided further insights into their expression and regulation during fruit development and ripening. Integrating our data, we conclude that the YST provides a valuable functional genomics tool for the identification of substantial numbers of novel secreted plant proteins that are associated with biological processes, including fruit development and ripening.

  13. New insights from an old mutant: SPADIX4 governs fruiting body development but not hyphal fusion in Sordaria macrospora.

    Science.gov (United States)

    Teichert, Ines; Lutomski, Miriam; Märker, Ramona; Nowrousian, Minou; Kück, Ulrich

    2017-02-01

    During the sexual life cycle of filamentous fungi, multicellular fruiting bodies are generated for the dispersal of spores. The filamentous ascomycete Sordaria macrospora has a long history as a model system for studying fruiting body formation, and two collections of sterile mutants have been generated. However, for most of these mutants, the underlying genetic defect remains unknown. Here, we investigated the mutant spadix (spd) that was generated by X-ray mutagenesis in the 1950s and terminates sexual development after the formation of pre-fruiting bodies (protoperithecia). We sequenced the spd genome and found a 22 kb deletion affecting four genes, which we termed spd1-4. Generation of deletion strains revealed that only spd4 is required for fruiting body formation. Although sterility in S. macrospora is often coupled with a vegetative hyphal fusion defect, Δspd4 was still capable of fusion. This feature distinguishes SPD4 from many other regulators of sexual development. Remarkably, GFP-tagged SPD4 accumulated in the nuclei of vegetative hyphae and fruiting body initials, the ascogonial coils, but not in sterile tissue from the developing protoperithecium. Our results point to SPD4 as a specific determinant of fruiting body formation. Research on SPD4 will, therefore, contribute to understanding cellular reprogramming during initiation of sexual development in fungi.

  14. Mining secreted proteins that function in pepper fruit development and ripening using a yeast secretion trap (YST)

    International Nuclear Information System (INIS)

    Lee, Je Min; Lee, Sang-Jik; Rose, Jocelyn K.C.; Yeam, Inhwa; Kim, Byung-Dong

    2014-01-01

    Highlights: • Yeast secretion trap (YST) is a valuable tool for mining secretome. • A total of 80 secreted proteins are newly identified via YST in pepper fruits. • The secreted proteins are differentially regulated during pepper development and ripening. • Transient GFP-fusion assay and in planta secretion trap can effectively validate the secretion of proteins. - Abstract: Plant cells secrete diverse sets of constitutively- and conditionally-expressed proteins under various environmental and developmental states. Secreted protein populations, or secretomes have multiple functions, including defense responses, signaling, metabolic processes, and developmental regulation. To identify genes encoding secreted proteins that function in fruit development and ripening, a yeast secretion trap (YST) screen was employed using pepper (Capsicum annuum) fruit cDNAs. The YST screen revealed 80 pepper fruit-related genes (CaPFRs) encoding secreted proteins including cell wall proteins, several of which have not been previously described. Transient GFP-fusion assay and an in planta secretion trap were used to validate the secretion of proteins encoded by selected YST clones. In addition, RNA gel blot analyses provided further insights into their expression and regulation during fruit development and ripening. Integrating our data, we conclude that the YST provides a valuable functional genomics tool for the identification of substantial numbers of novel secreted plant proteins that are associated with biological processes, including fruit development and ripening

  15. Development and optimisation by means of sensory analysis of new beverages based on different fruit juices and sherry wine vinegar.

    Science.gov (United States)

    Cejudo-Bastante, María Jesús; Rodríguez Dodero, M Carmen; Durán Guerrero, Enrique; Castro Mejías, Remedios; Natera Marín, Ramón; García Barroso, Carmelo

    2013-03-15

    Despite the long history of sherry wine vinegar, new alternatives of consumption are being developed, with the aim of diversifying its market. Several new acetic-based fruit juices have been developed by optimising the amount of sherry wine vinegar added to different fruit juices: apple, peach, orange and pineapple. Once the concentrations of wine vinegar were optimised by an expert panel, the aforementioned new acetic fruit juices were tasted by 86 consumers. Three different aspects were taken into account: habits of consumption of vinegar and fruit juices, gender and age. Based on the sensory analysis, 50 g kg(-1) of wine vinegar was the optimal and preferred amount of wine vinegar added to the apple, orange and peach juices, whereas 10 g kg(-1) was the favourite for the pineapple fruit. Based on the olfactory and gustatory impression, and 'purchase intent', the acetic beverages made from peach and pineapple juices were the most appreciated, followed by apple juice, while those obtained from orange juice were the least preferred by consumers. New opportunities for diversification of the oenological market could be possible as a result of the development of this type of new product which can be easily developed by any vinegar or fruit juice maker company. © 2012 Society of Chemical Industry.

  16. Management of Soilborne Diseases in Strawberry Using Vegetable Rotations

    OpenAIRE

    Subbarao, Krishna V

    2007-01-01

    The influence of crop rotation on soilborne diseases and yield of strawberry (Fragaria × ananassa) was determined at a site infested with Verticillium dahliae microsclerotia and at another with no known history of V. dahliae infestation during 1997 to 2000. The rotations studied at the V. dahliae-infested site were (i) broccoli-broccoli-strawberry, (ii) Brussels sproutsstrawberry, and (iii) lettuce-lettuce-strawberry; the treatments at the site with no history of V. dahliae were (i) broccoli-...

  17. Development of Rhagoletis pomonella and Rhagoletis indifferens (Diptera: Tephritidae)in mango and other tropical and temperate fruit in the laboratory

    Science.gov (United States)

    Temperate fruit flies in the genus Rhagoletis (Diptera: Tephritidae) have narrow host ranges relative to those of tropical fruit flies, suggesting they will not attack or are incapable of developing in most novel fruit. Here we tested the hypothesis that apple maggot fly, Rhagoletis pomonella (Wals...

  18. Occurrence of parasites on fruits and vegetables in Norway.

    Science.gov (United States)

    Robertson, L J; Gjerde, B

    2001-11-01

    Between August 1999 and January 2001, samples of various fruits and vegetables obtained within Norway were analyzed by published methods for parasite contamination. Neither Cyclospora oocysts nor Ascaris (or other helminth) eggs were detected on any of the samples examined for these parasites. However, of the 475 samples examined for Cryptosporidium oocysts and Giardia cysts, 29 (6%) were found to be positive. No samples were positive for both parasites. Of the 19 Cryptosporidium-positive samples. 5 (26%) were in lettuce, and 14 (74%) in mung bean sprouts. Of the 10 Giardia-positive samples, 2 (20%) were in dill, 2 (20%) in lettuce, 3 (30%) in mung bean sprouts, 1 (10%) in radish sprouts, and 2 (20%) in strawberries. Mung bean sprouts were significantly more likely to be contaminated with Cryptosporidium oocysts or Giardia cysts than the other fruits and vegetables. Concentrations of Cryptosporidium and Giardia detected were generally low (mean of approximately 3 [oo]cysts per 100 g produce). Although some of the contaminated produce was imported (the majority, if sprouted seeds are excluded), there was no association between imported produce and detection of parasites. Crvptosporidium oocysts and Giardia cysts were also detected in water samples concerned with field irrigation and production of bean sprouts within Norway. This is the first time that parasites have been detected on vegetables and fruit obtained in a highly developed. wealthy country, without there being an outbreak situation. These findings may have important implications for global food safety.

  19. Heat unit accumulation and inflorescence and fruit development in ‘Ubá’ mango trees grown in Visconde do Rio Branco-MG

    Directory of Open Access Journals (Sweden)

    Lorena Moreira Carvalho Lemos

    2018-04-01

    Full Text Available Abstract There are little information in the scientific literature on flowering and fruiting of ‘Ubá’ mango trees. These information enables to know the proportion of hermaphrodite flowers in inflorescence, fruit set percentage and developmental stages of the fruit. In this study evaluations on inflorescence and fruit development of the ‘Ubá’ mango tree (Mangifera indica L. were carried out, as well as the determination of the required number of heat units for full fruit development. Thirty branches whose terminal buds were swollen were selected from five mango trees. With the aid of a camera and a caliper, the panicle and fruit development were evaluated weekly until full fruit development. A digital thermometer was used to record ambient temperatures during fruit development in order to estimate the number of heat units required for complete development of the fruits. Male and hermaphrodite flowers of the panicles were also identified and counted. The developmental cycle of ‘Ubá’ mango from the beginning of apical bud swelling to commercial harvest of the fruit lasted 168 days in 2011 and 154 days in 2012. The number of hermaphrodite flowers and the percentage of fruit set in the inflorescence in 2011 were 32.3 and 0.066%, respectively; and 122.1 and 0.099% in 2012, respectively. There was accumulation of 3,173 heat units from flower bud swelling to full development of the ‘Ubá’ mangoes.

  20. Gypsum as a source of sulfur for strawberry cropsGesso agricola como fonte de enxofre para a cultura do morango

    Directory of Open Access Journals (Sweden)

    Universidade Estadual do Centro-Oeste

    2013-09-01

    Full Text Available De uma maneira geral, diversos são os estudos sobre a resposta das plantas ao enxofre (S, porém poucos são específicos para a cultura do morangueiro. A exigência por nutrientes e o estado nutricional é um atributo particular de cada espécie e deve ser levada em conta na determinação da demanda nutricional. O objetivo deste trabalho foi avaliar a influência da aplicação de doses S e fósforo (P no desenvolvimento e produtividade do morangueiro. Foi conduzido um experimento em casa de vegetação, em vasos de 2 Kg de solo (Latossolo Bruno, em Guarapuava-PR. O experimento constituiu-se de um fatorial 6 x 2, delineado em blocos ao acaso, com três repetições, sendo seis doses de S: 0, 10, 20, 30, 40 e 50 mg kg-1, na forma de gesso agrícola e duas doses de P: 300 e 600 mg kg-1 de P2O5. Nos vasos foram transplantadas mudas de morango variedade Albion, cultivadas por 170 dias. Os frutos foram colhidos e pesados ao longo do experimento e a parte aérea ao final do experimento. A parte aérea e os frutos foram secos, pesados, moídos e analisados em relação ao teor de S, Ca e P. A produção de frutos de morango foi influenciada pelas doses de S, sendo que a maior produtividade foi alcançada na dose de 60 mg kg-1 de S com aplicação de 300 mg kg-1 de P e na aplicação de 600 mg kg-1 de P combinada com a dose de 37 mg kg-1 de S. There are several studies on plant responses to Sulfur (S, but few are specific to the strawberry crop. The requirement for nutrients and the nutritional status of plants is a particular attribute of each species that should be taken into account when determining the plant’s nutritional demand. The objective of this study was to evaluate the application of S and phosphate (P rates in the development and productivity of strawberry crops. An experiment was conducted in a greenhouse in pots containing 2 kg soil (Oxisol in Guarapuava city of Paraná State. The experiment consisted of a 6 × 2 factorial outlined

  1. Changes in ABA, IAA and JA levels during calyx, fruit and leaves development in cape gooseberry plants (Physalis peruviana L.).

    Science.gov (United States)

    Álvarez-Flórez, F; López-Cristoffanini, C; Jáuregui, O; Melgarejo, L M; López-Carbonell, M

    2017-06-01

    Changes in abscisic acid (ABA), indole-3-acetic acid (IAA) and jasmonic acid (JA) content in developing calyx, fruits and leaves of Physalis peruviana L. plants were analysed. Plant hormones have been widely studied for their roles in the regulation of various aspects related to plant development and, in particular, into their action during development and ripening of fleshly fruits. The obtained evidences suggest that the functions of these hormones are no restricted to a particular development stage, and more than one hormone is involved in controlling various aspects of plant development. Our results will contribute to understand the role of these hormones during growth and development of calyx, fruits and leaves in cape gooseberry plants. This work offers a good, quickly and efficiently protocol to extract and quantify simultaneously ABA, IAA and JA in different tissues of cape gooseberry plants. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  2. Food selection in larval fruit flies: dynamics and effects on larval development

    Science.gov (United States)

    Schwarz, Sebastian; Durisko, Zachary; Dukas, Reuven

    2014-01-01

    Selecting food items and attaining a nutritionally balanced diet is an important challenge for all animals including humans. We aimed to establish fruit fly larvae ( Drosophila melanogaster) as a simple yet powerful model system for examining the mechanisms of specific hunger and diet selection. In two lab experiments with artificial diets, we found that larvae deprived of either sucrose or protein later selectively fed on a diet providing the missing nutrient. When allowed to freely move between two adjacent food patches, larvae surprisingly preferred to settle on one patch containing yeast and ignored the patch providing sucrose. Moreover, when allowed to move freely between three patches, which provided either yeast only, sucrose only or a balanced mixture of yeast and sucrose, the majority of larvae settled on the yeast-plus-sucrose patch and about one third chose to feed on the yeast only food. While protein (yeast) is essential for development, we also quantified larval success on diets with or without sucrose and show that larvae develop faster on diets containing sucrose. Our data suggest that fruit fly larvae can quickly assess major nutrients in food and seek a diet providing a missing nutrient. The larvae, however, probably prefer to quickly dig into a single food substrate for enhanced protection over achieving an optimal diet.

  3. Developing a Hybrid Solar/Wind Powered Drip Irrigation System for Dragon Fruit Yield

    Science.gov (United States)

    Widiastuti, I.; Wijayanto, D. S.

    2017-03-01

    Irrigation operations take a large amount of water and energy which impact to total costs of crop production. Development of an efficient irrigation supplying precise amount of water and conserving the use of energy can have benefits not only by reducing the operating costs but also by enhancing the farmland productivity. This article presents an irrigation method that promotes sustainable use of water and energy appropriate for a developing tropical country. It proposes a drip irrigation system supported by a combined solar-wind electric power generation system for efficient use of water in dragon fruit cultivation. The electric power generated is used to drive a water pump filling a storage tank for irrigating a 3000 m2 dragon fruit yield in Nguntoronadi, Wonogiri, Indonesia. In designing the irrigation system, the plant’s water requirement was identified based on the value of reference evapotranspiration of the area. A cost/benefit analysis was performed to evaluate the economic feasibility of the proposed scheme. The installation of this solar and wind drip irrigation helps provide sufficient quantity of water to each plant using renewable energy sources which reduce dependence on fossil fuel.

  4. The genome sequence and transcriptome of Potentilla micrantha and their comparison to Fragaria vesca (the woodland strawberry).

    Science.gov (United States)

    Buti, Matteo; Moretto, Marco; Barghini, Elena; Mascagni, Flavia; Natali, Lucia; Brilli, Matteo; Lomsadze, Alexandre; Sonego, Paolo; Giongo, Lara; Alonge, Michael; Velasco, Riccardo; Varotto, Claudio; Šurbanovski, Nada; Borodovsky, Mark; Ward, Judson A; Engelen, Kristof; Cavallini, Andrea; Cestaro, Alessandro; Sargent, Daniel James

    2018-04-01

    The genus Potentilla is closely related to that of Fragaria, the economically important strawberry genus. Potentilla micrantha is a species that does not develop berries but shares numerous morphological and ecological characteristics with Fragaria vesca. These similarities make P. micrantha an attractive choice for comparative genomics studies with F. vesca. In this study, the P. micrantha genome was sequenced and annotated, and RNA-Seq data from the different developmental stages of flowering and fruiting were used to develop a set of gene predictions. A 327 Mbp sequence and annotation of the genome of P. micrantha, spanning 2674 sequence contigs, with an N50 size of 335,712, estimated to cover 80% of the total genome size of the species was developed. The genus Potentilla has a characteristically larger genome size than Fragaria, but the recovered sequence scaffolds were remarkably collinear at the micro-syntenic level with the genome of F. vesca, its closest sequenced relative. A total of 33,602 genes were predicted, and 95.1% of bench-marking universal single-copy orthologous genes were complete within the presented sequence. Thus, we argue that the majority of the gene-rich regions of the genome have been sequenced. Comparisons of RNA-Seq data from the stages of floral and fruit development revealed genes differentially expressed between P. micrantha and F. vesca.The data presented are a valuable resource for future studies of berry development in Fragaria and the Rosaceae and they also shed light on the evolution of genome size and organization in this family.

  5. Strawberries packaged with Polyvinyl Chloride (PVC film/ Morangos embalados com filme de Ppolicloreto de Vinila (PVC

    Directory of Open Access Journals (Sweden)

    Sergio Ruffo Roberto

    2006-06-01

    Full Text Available Strawberries cv. Dover with approximately 75% of their surface with red color was packaged in disposable polyethylene terephthalate (PET container and sealed with PVC film of 15mm thick. Strawberries without film serves as control. The fruits were stored at 12°C for ten days and there were determined during storage time the titratable acidity, solid soluble and vitamin C contents, color, respiration rate, texture and weight loss. After 6 days the strawberries packaged without film had decayed while the fruits packaged with PVC film were in conditions of consumption and commercialization. After 10 days all fruits were inappropriate to consumption. Packages with PVC film extended the shelf life of strawberries preserving the color characteristic of the product and vitamin C content, reducing the respiration rate and weight loss, characterizing this packaging as being adequate to this kind of product.Foram utilizados morangos cv. Dover com aproximadamente 75% de sua superfície com coloração vermelha e embalados em potes de polietileno tereftalato (PET. No tratamento CONTROLE os morangos foram acondicionados sem filme e no tratamento FILME, as embalagens foram seladas com filme de PVC de 15mm de espessura. As frutas foram armazenadas a 12°C por dez dias e foram determinadas ao longo do tempo acidez titulável, sólidos solúveis, vitamina C, cor, taxa de respiração, textura e perda de massa. Após 6 dias de armazenagem os morangos CONTROLE estavam impróprios para consumo pois apresentavam deterioração visível por bolores enquanto que os embalados com PVC estavam em condições de consumo e comercialização. Após 10 dias todos os morangos estavam impróprios para consumo. A embalagem utilizando filme de PVC aumentou a vida de prateleira de morangos em relação aos sem embalagem pois preservou melhor a coloração característica do produto e teor de vitamina C e reduziu as taxas de respiração e de perda de massa, caracterizando a

  6. Carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents.

    Science.gov (United States)

    Nakkanong, Korakot; Yang, Jing Hua; Zhang, Ming Fang

    2012-06-13

    Carotenoid levels and composition during squash fruit development were compared in Cucurbita moschata , Cucurbita maxima , and two lines of their interspecific inbred lines, namely, Maxchata1 and Maxchata2. Eight genes associated with carotenoid biosynthesis were analyzed by quantitative RT-PCR. The two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. C. moschata had the lowest total carotenoid content and mainly accumulated α-carotene and β-carotene, as expected in a fruit with pale-orange flesh. Low carotenoid content in this species was probably due to the comparatively low expression of all genes investigated, especially PSY1 gene, compared to the other squashes. The predominant carotenoids in C. maxima were violaxanthin and lutein, which produced a corresponding yellow flesh color in mature fruit. The relationship between the expression of the CHYB and ZEP genes may result in almost equal concentrations of violaxanthin and lutein in C. maxima at fruit ripening. In contrast, their interspecific inbred lines principally accumulated lutein and β-carotene, leading to orange flesh color. The PSY1 gene exhibited higher expression levels at earlier stages of fruit development in the Maxchata lines, potentially triggering the increased carotenoid accumulation seen in these fruits. Likewise, the higher transcription level of CHYB gene observed in the two interspecific inbred lines might be correlated with high lutein in these hybrids. However, this study could not explain the observed β-carotene accumulation on the basis of gene expression.

  7. Relationship between delayed embryonic development and metabolic factors and fat deposition in fruit bat Cynopterus sphinx.

    Science.gov (United States)

    Banerjee, Arnab; Meenakumari, K J; Krishna, Amitabh

    2007-01-01

    The present study was undertaken in the fruit bat Cynopterus sphinx, which breeds twice in quick succession at Varanasi, India. Its gestation period varies significantly in the two successive pregnancies of the year owing to delayed embryonic development during the first (winter) pregnancy. The primary aim of the present study was to determine the role of metabolic factors in delayed embryonic development in the fruit bat C. sphinx. Variation in bodyweight, fat deposition, oxygen (O(2)) consumption rate, basal metabolic rate (BMR), body temperature (Tb) and hepatic succinate dehydrogenase (SDH) activity, along with circulating levels of thyroid hormones (tri-iodothyronine and thyroxine), were examined as metabolic factors during the two successive pregnancies in C. sphinx. The increase in bodyweight observed in November was due to accumulation of white adipose tissue in the posterior abdominal region. A significant decline in O(2) consumption rate, BMR, Tb and SDH activity was found in early winter in November-December, which coincides closely with the period of fat accumulation and with the period of delayed embryonic development in C. sphinx. A significantly higher O(2) consumption rate, BMR, Tb and SDH activity was noted during the second pregnancy in, when embryonic development was relatively faster. Thyroid hormone levels were high during the period of embryonic delay compared with levels during the remaining months. The results of the present study suggest that the delayed embryonic development in C. sphinx during early winter may be due to a low O(2) consumption rate, BMR, Tb and SDH activity in November-December. The energy saved by suppressing embryonic development in this species may be advantageous for fat accumulation. Increased thyroid hormone levels during the early winter period might facilitate fat accumulation in C. sphinx.

  8. Chlorophyll metabolism in pollinated vs. parthenocarpic fig fruits throughout development and ripening.

    Science.gov (United States)

    Rosianskey, Yogev; Dahan, Yardena; Yadav, Sharawan; Freiman, Zohar E; Milo-Cochavi, Shira; Kerem, Zohar; Eyal, Yoram; Flaishman, Moshe A

    2016-08-01

    Expression of 13 genes encoding chlorophyll biosynthesis and degradation was evaluated. Chlorophyll degradation was differentially regulated in pollinated and parthenocarpic fig fruits, leading to earlier chlorophyll degradation in parthenocarpic fruits. Varieties of the common fig typically yield a commercial summer crop that requires no pollination, although it can be pollinated. Fig fruit pollination results in larger fruit size, greener skin and darker interior inflorescence color, and slows the ripening process compared to non-pollinated fruits. We evaluated the effect of pollination on chlorophyll content and levels of transcripts encoding enzymes of the chlorophyll metabolism in fruits of the common fig 'Brown Turkey'. We cloned and evaluated the expression of 13 different genes. All 13 genes showed high expression in the fruit skin, inflorescences and leaves, but extremely low expression in roots. Pollination delayed chlorophyll breakdown in the ripening fruit skin and inflorescences. This was correlated with the expression of genes encoding enzymes in the chlorophyll biosynthesis and degradation pathways. Expression of pheophorbide a oxygenase (PAO) was strongly negatively correlated with chlorophyll levels during ripening in pollinated fruits; along with its high expression levels in yellow leaves, this supports a pivotal role for PAO in chlorophyll degradation in figs. Normalizing expression levels of all chlorophyll metabolism genes in the pollinated and parthenocarpic fruit skin and inflorescences showed three synthesis (FcGluTR1, FcGluTR2 and FcCLS1) and three degradation (FcCLH1, FcCLH2 and FcRCCR1) genes with different temporal expression in the pollinated vs. parthenocarpic fruit skin and inflorescences. FcCAO also showed different expressions in the parthenocarpic fruit skin. Thus, chlorophyll degradation is differentially regulated in the pollinated and parthenocarpic fruit skin and inflorescences, leading to earlier and more sustained

  9. Effects of Insecticides on Strawberry Aphid Chaetosiphon fragaefolii (Cockerell on Resistant and Susceptible Strawberry Genotypes

    Directory of Open Access Journals (Sweden)

    Slobodan Milenković

    2011-01-01

    Full Text Available Strawberry aphid, Chaetosiphon fragaefolii (Cockerell, is the most important vector ofstrawberry virus. Breeding of genotypes resistant to this pest is an important preventivecontrol measure, which can be compatible with rational insecticide application. The aimof the paper was to determine effects of dimethoate and deltamethrin on C. fragaefoliipopulations reared on two strawberry genotypes different in susceptibility: susceptiblestrawberry cultivar Čačanska rana and medium resistant hybrid, zf/1/94/96 (Senga Fructarinax Del Norte. Lower toxicity of deltamethrin was observed (laboratory assay as well aslower biological efficacy of dimethoate at lower concentrations (field trial for specimensfrom C. fragaefolii population reared on susceptible strawberry cultivar Čačanska rana.

  10. Economic feasibility of fruit and vegetable irradiation in Australia

    International Nuclear Information System (INIS)

    Beattie, B.B.; Wiblin, B.

    1983-01-01

    The cost of treatment of fruit and vegetables in an Ionising Radiation Treatment (IRT) facility can be quite low. However, other circumstances mitigate against its overall economic feasibility. Research has determined that irradiation treatments are technically successful in three general areas. These are: prevention of fungal wastage in strawberries, prevention of sprouting in potatoes and onions, and disinfestation of fruit and vegetables from insect pests. The strawberry industry in Australia is very small and marketing of the crop will continue regardless of the availability of an IRT facility. On the other hand the potato industry is large but does not see sprouting as a marketing problem. The third area is disinfestation against Queensland fruit fly for interstate and export markets. Recent changes in Victorian quarantine legislation give a much greater number of alternatives for produce going into that State

  11. The filamentous fungus Sordaria macrospora as a genetic model to study fruiting body development.

    Science.gov (United States)

    Teichert, Ines; Nowrousian, Minou; Pöggeler, Stefanie; Kück, Ulrich

    2014-01-01

    Filamentous fungi are excellent experimental systems due to their short life cycles as well as easy and safe manipulation in the laboratory. They form three-dimensional structures with numerous different cell types and have a long tradition as genetic model organisms used to unravel basic mechanisms underlying eukaryotic cell differentiation. The filamentous ascomycete Sordaria macrospora is a model system for sexual fruiting body (perithecia) formation. S. macrospora is homothallic, i.e., self-fertile, easily genetically tractable, and well suited for large-scale genomics, transcriptomics, and proteomics studies. Specific features of its life cycle and the availability of a developmental mutant library make it an excellent system for studying cellular differentiation at the molecular level. In this review, we focus on recent developments in identifying gene and protein regulatory networks governing perithecia formation. A number of tools have been developed to genetically analyze developmental mutants and dissect transcriptional profiles at different developmental stages. Protein interaction studies allowed us to identify a highly conserved eukaryotic multisubunit protein complex, the striatin-interacting phosphatase and kinase complex and its role in sexual development. We have further identified a number of proteins involved in chromatin remodeling and transcriptional regulation of fruiting body development. Furthermore, we review the involvement of metabolic processes from both primary and secondary metabolism, and the role of nutrient recycling by autophagy in perithecia formation. Our research has uncovered numerous players regulating multicellular development in S. macrospora. Future research will focus on mechanistically understanding how these players are orchestrated in this fungal model system. Copyright © 2014 Elsevier Inc. All rights reserved.

  12. Development of improved attractants and their integration into fruit fly SIT management programmes. Proceedings of a final research coordination meeting

    International Nuclear Information System (INIS)

    2007-10-01

    Information provided by trapping systems is used to assess the presence, seasonal abundance, spatial distribution, host sequence and infestation levels of fruit fly pests. This information is key for implementation of effective fruit fly control programmes. Most trapping systems commercially available are based on para-pheromones which are male specific. These male specific trapping systems have been used as the main survey tool in area-wide fruit fly control programmes. Nevertheless, in recent years, scientists and programme managers have realized that, in order to improve the efficiency of fruit fly control, it is essential to have a female specific or at least a female biased trapping system. Until the late 1990s, the only fruit fly female biased attractants were based on natural protein baits such as Torula Yeast and hydrolysate proteins. Although these attractants tend to catch more females than males (in average 60% females against 40% males), the proportion in favor of females is insufficient and the attractants are considered to be weak and non-selective. In 1999, as a result of a previous Coordinated Research Project (CRP) entitled 'Development of Female Medfly Attractant Systems for Trapping and Sterility Assessment' the first effective female biased synthetic food lure was developed for the Mediterranean fruit fly (Ceratitis capitata, Wied.) (IAEA-TECDOC-1099). This lure, with the commercial name of Biolure, is now being used in large-scale medfly control programmes worldwide. Given this background, and in order to further advance this field, the Joint FAO/IAEA Programme approved in 2000 a five year CRP entitled 'Development of Improved Attractants and their Integration into Fruit Fly SIT Management Programmes'. The research conducted under this CRP focused mainly on developing female biased trapping systems for other fruit fly species of quarantine and economic importance within the Anastrepha, Bactrocera, Ceratitis and Dacus genera and on optimization

  13. Physiological role of glucose-6-phosphate dehydrogenase in cold acclimation of strawberry (Fragaria × ananassa)

    Science.gov (United States)

    Zhang, Yong; Yu, Dingqun; Luo, Ya; Wang, Xiaorong; Chen, Qing; Sun, Bo; Wang, Yan; Liu, Zejing; Tang, Haoru

    2018-04-01

    In recent years, there has been an increasing interest in study of new resistance mechanism in fruit trees. All these regard the climate change and subsequent fruit production. Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the first and rate-limiting step of the oxidative pentose phosphate pathway (OPPP), and the expression of this enzyme is related to different biotic and abiotic stresses. Under accumulation of low temperature stress, the significant increase in G6PDH activity was found to be closely correlated to the levels of antioxidant enzymes, malondialdehyde (MDA) contents, sugar contents as well as changes of superoxide (O2•-). It is suggested that the enhancement of cold resistance of strawberry, which induced by cold acclimation, related to the significant increase in G6PDH activity. On one hand, G6PDH activates NADPH oxidase to generate reactive oxygen species (ROS); on the other hand, it may be involved in the activation of antioxidant enzymes, and accelerates many other important NADPH-dependent enzymatic reactions. Then further result in the elevation of membrane stability and cold resistance of strawberry. Interestingly, even though the plants were placed again under a temperature of 25°C for 1 d, the higher cold resistance, enzyme activities and soluble sugar content acquired.

  14. Health and taste related compounds in strawberries under various irrigation regimes and bio-stimulant application.

    Science.gov (United States)

    Kapur, Burcak; Sarıdaş, Mehmet Ali; Çeliktopuz, Eser; Kafkas, Ebru; Paydaş Kargı, Sevgi

    2018-10-15

    Strawberry has a unique status within the fruit species in terms of health and taste related compounds. This experimental study concerned the application of a bio-stimulant at various drip irrigation levels (IR125, IR100, IR75 and IR50). The effects of the bio-stimulant (seaweed extract) on the eating quality, i.e., the taste-related (TSS, fructose, glucose, sucrose and citric, malic, l-ascorbic acid), and health-related (antioxidant activity, total phenol, myricetin and quercetin) compounds were studied in two strawberry cultivars. The 'Rubygem' with its higher sugar and lower acid content has been more preferable than the 'Kabarla' cultivar. The bio-stimulant contributes to taste by improving the TSS, fructose, sucrose and also to health by increasing the quercetin content of the fruit which is associated to the cardiovascular properties and cancer reducing agents. The experiment conducted revealed significant increases only in the TSS contents and antioxidant activity under the IR50 and IR75 deficit irrigation treatments. Copyright © 2018 Elsevier Ltd. All rights reserved.

  15. Mucilage extraction and substrates in the seedling development of yellow passion fruit plants

    Directory of Open Access Journals (Sweden)

    Ricardo Sfeir Aguiar

    2014-02-01

    Full Text Available The object of this work was to evaluate different methods of mucilage extraction and substrates on passion fruit seedling emergence and development , in a mist chamber. Five methods of mucilage extraction were used: water, water + sand, water + virgin whitewash; blender with protected blades and fermentation in water, and three different types of substrates: rice hull, vermiculite and coconut fiber. The experiment had a completely randomized design with five replications in a factorial 5 x 3 scheme (5 extraction methods of seed mucilage and 3 substrates being each parcel composed of 25 seeds. The parameters evaluated were: seedling emergence, speed of emergence index, leaf number, stem length, longest root length, weight of dry matter of roots and shoots. Water and fermentation in water are the best method for mucilage extraction and rice hull and coconut fiber are the best substrate for passionfruit seedling emergence and development.

  16. Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

    Directory of Open Access Journals (Sweden)

    Rafael Gustavo Ferreira Morales

    2011-12-01

    Full Text Available Most strawberry (Fragaria × ananassa Duchesne cultivars used in Brazil are developed in other countries, it became clear the need to start the strawberry breeding program in the country. To start a breeding program is necessary the genetic characterization of the germplasm available. Molecular markers are important tools that can be used for this purpose. The objectives of the present study were to assess the genetic similarity among 11 strawberry cultivars using RAPD and ISSR molecular markers and to indicate the possible promising crosses. The DNA of the eleven strawberry cultivars was extracted and amplified by PCR with RAPD and ISSR primers. The DNA fragments were separated in agarose gel for the RAPD markers and in polyacrylamide gel for the ISSR markers. The genetic similarity matrix was estimated by the Jaccard coefficient. Based on this matrix, the cultivars were grouped using the UPGMA method. The dendogram generated by the RAPD markers distributed the cultivars in three groups while the ISSR markers generated two groups. There was no direct relationship between the marker groups when the two types of markers were compared. The grouping proposed by the ISSR markers was more coherent with the origin and the genealogy of the cultivars than that proposed by the RAPD markers, and it can be considered the most efficient method for the study of genetic divergence in strawberry. The most promising crosses, based on the genetic divergence estimated from the RAPD and ISSR molecular data were between the Tudla and Ventana and the Oso Grande and Ventana cultivars, respectively.

  17. Cambios en la Actividad Antioxidante Durante el Desarrollo de Frutos de Psidium cattleianum Sabine (Myrtaceae / Changes in the Antioxidant Activity During the Development in Fruits of Psidium cattleianum Sabine (Myrtaceae

    Directory of Open Access Journals (Sweden)

    Villacorta Lozano Verónica

    2013-08-01

    Full Text Available Resumen. Psidium cattleianum Sabine, conocida como casdulce, guayaba japonesa, guayaba peruana y guayaba fresa, es un árbol originario de América del Sur. Estudios preliminares en esta especie han sugerido una elevada actividad antioxidante, un alto contenido fenólico y un gran potencial nutricional y funcional. En esta investigación se analizó la evolución de varios componentes del sistema de defensa antioxidante no enzimático, durante el crecimiento y maduración de los frutos de Psidium cattleianum Sabine. El periodo de crecimiento de los frutos presentó la actividad antioxidante más elevada, medida a través de las técnicas TEAC-DPPH y TEAC-ABTS (Trolox equivalent antioxidant capacity, FRAP (Ferric reducing/antioxidant powery ORAC (Oxigen Radical Absorbance Capacity, así como elcontenido máximo de fenoles totales, entre los que destacaron los ácidos clorogénico, cafeico y p-coumárico, y de sustancias no polifenólicas como el ácido ascórbico. Durante el periodo de maduración, se observó una disminución de la actividad antioxidante, posiblemente promovida por la pérdida gradual de algunos compuestos polifenólicos, así como también, debido a la aparición de otros compuestos como las antocianinas. Los resultados mostraron que estos frutos contienen un alto contenido de sustancias con actividad antioxidante, lo cual soporta el desarrollo de esta especie como nueva alternativa productiva / Abstract. Psidium cattleianum Sabine, known as cas sweet, japanese guava, peruvian guava and strawberry guava, is a tree native to South America. Preliminary studies in this specieshave suggested a high antioxidant activity, a high phenoliccontent and great nutritional and functional potential. In thisstudy, the evolution of some components of the non-enzimatic antioxidant defense system was analized during growth and ripening of fruit of Psidium cattleianum Sabine. The growing period of the fruits presented the highest antioxidant

  18. Strawberry Phytochemicals Inhibit Azoxymethane/Dextran Sodium Sulfate-Induced Colorectal Carcinogenesis in Crj: CD-1 Mice

    Directory of Open Access Journals (Sweden)

    Ni Shi

    2015-03-01

    Full Text Available Human and experimental colon carcinogenesis are enhanced by a pro-inflammatory microenvironment. Pharmacologically driven chemopreventive agents and dietary variables are hypothesized to have future roles in the prevention of colon cancer by targeting these processes. The current study was designed to determine the ability of dietary lyophilized strawberries to inhibit inflammation-promoted colon carcinogenesis in a preclinical animal model. Mice were given a single i.p. injection of azoxymethane (10 mg kg−1 body weight. One week after injection, mice were administered 2% (w/v dextran sodium sulfate in drinking water for seven days and then an experimental diet containing chemically characterized lyophilized strawberries for the duration of the bioassay. Mice fed control diet, or experimental diet containing 2.5%, 5.0% or 10.0% strawberries displayed tumor incidence of 100%, 64%, 75% and 44%, respectively (p < 0.05. The mechanistic studies demonstrate that strawberries reduced expression of proinflammatory mediators, suppressed nitrosative stress and decreased phosphorylation of phosphatidylinositol 3-kinase, Akt, extracellular signal-regulated kinase and nuclear factor kappa B. In conclusion, strawberries target proinflammatory mediators and oncogenic signaling for the preventive efficacies against colon carcinogenesis in mice. This works supports future development of fully characterized and precisely controlled functional foods for testing in human clinical trials for this disease.

  19. Cadmium toxicity affects chlorophyll a and b content, antioxidant enzyme activities and mineral nutrient accumulation in strawberry

    Directory of Open Access Journals (Sweden)

    Ferhad Muradoglu

    2015-01-01

    Full Text Available BACKGROUND: Cadmium (Cd is well known as one of the most toxic metals affecting the environment and can severely restrict plant growth and development. In this study, Cd toxicities were studied in strawberry cv. Camarosa using pot experiment. Chlorophyll and malondialdehyde (MDA contents, catalase (CAT, superoxide dismutase (SOD, ascorbate peroxidase (APX activities and mineral nutrient concentrations were investigated in both roots and leaves of strawberry plant after exposure Cd. RESULTS: Cd content in both roots and leaves was increased with the application of increasing concentrations of Cd. We found higher Cd concentration in roots rather than in leaves. Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased Cd concentration treatments in both roots and leaves. SOD and CAT activities was also increased with the increase Cd concentrations. K, Mn and Mg concentrations were found higher in leaves than roots under Cd stress. In general, increased Cd treatments increased K, Mg, Fe, Ca, Cu and Zn concentration in both roots and leaves. Excessive Cd treatments reduced chlorophyll contents, increased antioxidant enzyme activities and changes in plant nutrition concentrations in both roots and leaves. CONCLUSION: The results presented in this work suggested that Cd treatments have negative effect on chlorophyll content and nearly decreased 30% of plant growth in strawberry. Strawberry roots accumulated higher Cd than leaves. We found that MDA and antioxidant enzyme (CAT, SOD and APX contents may have considered a good indicator in determining Cd tolerance in strawberry plant.

  20. STRAWBERRY (FRAGARIA X ANANASSA DUCH LEAF ANTIOXIDATIVE RESPONSE TO BIOSTIMULATORS AND REDUCED FERTILIZATION WITH N AND K

    Directory of Open Access Journals (Sweden)

    Marija Špoljarević

    2010-06-01

    Full Text Available Strawberry cultivar Elsanta was grown in peat based substrate in a green house. Full dose and 50% reduced nitrogen and potassium fertilization were applied during fruit bearing period in spring, along with biostimulators Viva®, Megafol® and their combination. The specific activities of guaiacol peroxidase (GPXs; EC 1.11.1.7, catalase (CATs; EC 1.11.1.6, ascorbate peroxidase (APXs; EC 1.11.1.11 and glutathione reductase (GRs; EC 1.6.4.2 in strawberry leaf were stimulated by biostimulators and reduced fertilization. The strongest link seen here was between the enzymes of ascorbate-glutathione cycle (APXs and GRs, which were positively related to trifoliate leaf fresh mass (TLFM. The highest TLFM was observed in Megafol® treated plants.

  1. Development of technology for plantlet propagation by tissue culture. (3). Strawberry callus growth and changes in pH in liquid medium; Soshiki baiyo ni yoru shubyo tairyo zoshoku gijutsu no kaihatsu. (3). Ekitai baiyo ni okeru ichigo callus no zoshoku to baichi no pH henka

    Energy Technology Data Exchange (ETDEWEB)

    Yoshihara, T; Hanyo, H [Central Research Inst. of Electric Power Industry, Tokyo (Japan)

    1991-02-01

    Development of strawberry cultivation plants is described as a part of night-time power utilization activities for power load levelling. Strawberry calluses (undifferent tissue mass) cultured in a liquid medium reach the fastest growth period in one week and the steady state in four weeks. The callus growth shows the maximum value at this time, which was 20 times as much of the seedling. The medium pH changed in a range from 4 to 7. If the initial pH is 4.0 or higher, no difference is created in the callus propagation in the steady state period, but at 3.0, no propagation whatsoever. The pH after the fastest growth period converged to a range from 6.0 to 7.0, with the exception of initial pH at 3.0. The medium pH decreased as a result of pre-culture heating sterilization, formation of iron phosphate due to light irradiation, and organic acid release during the initial growth phase. The pH increased because of difference in the speed of absorbing ammonium and nitric acid during the later growth phase. The growth efficiency of 20 times is about the same as other plants. Since the pH change is maintained within the range from 4 to 7, which causes no difference in in growth, there is no need of adjusting the pH within this range. 18 refs., 15 figs., 3 tabs.

  2. Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening

    OpenAIRE

    Zhu, Qianglong; Gao, Peng; Liu, Shi; Zhu, Zicheng; Amanullah, Sikandar; Davis, Angela R.; Luan, Feishi

    2017-01-01

    Background Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an economically important crop with an attractive ripe fruit that has colorful flesh. Fruit ripening is a complex, genetically programmed process. Results In this study, a comparative transcriptome analysis was performed to identify the regulators and pathways that are involved in the fruit ripening of pale-yellow-flesh cultivated watermelon (COS) and red-flesh cultivated watermelon (LSW177). We first identified 797 novel g...

  3. Strawberry Production in Soilless Substrate Troughs – Plant Growth

    Science.gov (United States)

    Soilless substrates made of peat moss, coconut coir, perlite, rockwool or bark are pathogen free and they have been used in strawberry production in Europe in troughs or containers. Open field strawberry production in soilless substrate is new to California growers. The objective of this study was t...

  4. LED Light to improve Strawberry Flavour, Quality and Production

    NARCIS (Netherlands)

    Hanenberg, M.A.A.; Janse, J.; Verkerke, W.

    2016-01-01

    A current demonstration in the greenhouses of Wageningen UR in Bleiswijk (the Netherlands) shows positive results of LED light on the quality and production of strawberries (Fragaria x ananassa). In this demonstration two strawberry cultivars, Elsanta and Sonata, are exposed to three different light

  5. Programmed flowering of the F1 long-day strawberry cultivar ‘Elan’ with nitrogen and daylength manipulation

    Directory of Open Access Journals (Sweden)

    Edward F. Durner

    2015-12-01

    Full Text Available Consumer demand for locally grown, high quality strawberries is increasing even though California, Florida and Mexico provide a year-round source of strawberries for the world market. In most of the US, locally grown strawberries are only available for a short time in late spring from seasonal fruiting short-day cultivars thus off-season strawberry production is an attractive option for growers. Seed propagated hybrids such as ‘Elan’ are becoming more widely available and offer an alternative to often low yielding cultivars used for off-season production. To determine whether or not ‘Elan’ could be programmed to flower with photoperiod or nitrogen, seedlings were fertilized with either 100 or 800 ppm nitrogen for 4 weeks in September beginning one week after exposure to either short days, the natural photoperiod, or long days, the natural photoperiod supplemented with 24 hours of incandescent radiation. Plants were then greenhouse forced under both photoperiods and floral phenology evaluated. Elevated nitrogen during floral initiation in September enhanced and accelerated flowering and plants receiving elevated nitrogen during initiation under long days flowered more than any other treatment. To determine whether or not flowering could be enhanced a second time in the same plants, another 4 week period of elevated nitrogen was provided in December and plant phenology evaluated through mid-January. Elevated nitrogen (800 ppm in December enhanced December and January flowering. Seedlings were conditioned with elevated nitrogen for a third time in late spring then field planted (early summer on raised beds with white or black plastic mulch. Elevated nitrogen in late spring enhanced yield in field production. No effects of mulch color, initiation photoperiod or interaction of considered factors were detected. Flowering differences detected in greenhouse studies translated into differences observed in the production field suggesting

  6. Partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defence responses in wild strawberry (Fragaria vesca).

    Science.gov (United States)

    Osorio, Sonia; Castillejo, Cristina; Quesada, Miguel A; Medina-Escobar, Nieves; Brownsey, Geoff J; Suau, Rafael; Heredia, Antonio; Botella, Miguel A; Valpuesta, Victoriano

    2008-04-01

    In addition to the role of the cell wall as a physical barrier against pathogens, some of its constituents, such as pectin-derived oligogalacturonides (OGA), are essential components for elicitation of defence responses. To investigate how modifications of pectin alter defence responses, we expressed the fruit-specific Fragaria x ananassa pectin methyl esterase FaPE1 in the wild strawberry Fragaria vesca. Pectin from transgenic ripe fruits differed from the wild-type with regard to the degree and pattern of methyl esterification, as well as the average size of pectin polymers. Purified oligogalacturonides from the transgenic fruits showed a reduced degree of esterification compared to oligogalacturonides from wild-type fruits. This reduced esterification is necessary to elicit defence responses in strawberry. The transgenic F. vesca lines had constitutively activated pathogen defence responses, resulting in higher resistance to the necrotropic fungus Botrytis cinerea. Further studies in F. vesca and Nicotiana benthamiana leaves showed that the elicitation capacity of the oligogalacturonides is more specific than previously envisaged.

  7. Genome-Wide Identification and Evaluation of Reference Genes for Quantitative RT-PCR Analysis during Tomato Fruit Development.

    Science.gov (United States)

    Cheng, Yuan; Bian, Wuying; Pang, Xin; Yu, Jiahong; Ahammed, Golam J; Zhou, Guozhi; Wang, Rongqing; Ruan, Meiying; Li, Zhimiao; Ye, Qingjing; Yao, Zhuping; Yang, Yuejian; Wan, Hongjian

    2017-01-01

    Gene expression analysis in tomato fruit has drawn increasing attention nowadays. Quantitative real-time PCR (qPCR) is a routine technique for gene expression analysis. In qPCR operation, reliability of results largely depends on the choice of appropriate reference genes (RGs). Although tomato is a model for fruit biology study, few RGs for qPCR analysis in tomato fruit had yet been developed. In this study, we initially identified 38 most stably expressed genes based on tomato transcriptome data set, and their expression stabilities were further determined in a set of tomato fruit samples of four different fruit developmental stages (Immature, mature green, breaker, mature red) using qPCR analysis. Two statistical algorithms, geNorm and Normfinder, concordantly determined the superiority of these identified putative RGs. Notably, SlFRG05 (Solyc01g104170), SlFRG12 (Solyc04g009770), SlFRG16 (Solyc10g081190), SlFRG27 (Solyc06g007510), and SlFRG37 (Solyc11g005330) were proved to be suitable RGs for tomato fruit development study. Further analysis using geNorm indicate that the combined use of SlFRG03 (Solyc02g063070) and SlFRG27 would provide more reliable normalization results in qPCR experiments. The identified RGs in this study will be beneficial for future qPCR analysis of tomato fruit developmental study, as well as for the potential identification of optimal normalization controls in other plant species.

  8. Genome-Wide Identification and Evaluation of Reference Genes for Quantitative RT-PCR Analysis during Tomato Fruit Development

    Directory of Open Access Journals (Sweden)

    Yuan Cheng

    2017-08-01

    Full Text Available Gene expression analysis in tomato fruit has drawn increasing attention nowadays. Quantitative real-time PCR (qPCR is a routine technique for gene expression analysis. In qPCR operation, reliability of results largely depends on the choice of appropriate reference genes (RGs. Although tomato is a model for fruit biology study, few RGs for qPCR analysis in tomato fruit had yet been developed. In this study, we initially identified 38 most stably expressed genes based on tomato transcriptome data set, and their expression stabilities were further determined in a set of tomato fruit samples of four different fruit developmental stages (Immature, mature green, breaker, mature red using qPCR analysis. Two statistical algorithms, geNorm and Normfinder, concordantly determined the superiority of these identified putative RGs. Notably, SlFRG05 (Solyc01g104170, SlFRG12 (Solyc04g009770, SlFRG16 (Solyc10g081190, SlFRG27 (Solyc06g007510, and SlFRG37 (Solyc11g005330 were proved to be suitable RGs for tomato fruit development study. Further analysis using geNorm indicate that the combined use of SlFRG03 (Solyc02g063070 and SlFRG27 would provide more reliable normalization results in qPCR experiments. The identified RGs in this study will be beneficial for future qPCR analysis of tomato fruit developmental study, as well as for the potential identification of optimal normalization controls in other plant species.

  9. Evolution of a Development Model for Fruit Industry against Background of an Aging Population: Intensive or Extensive Adjustment?

    Directory of Open Access Journals (Sweden)

    Bin Yuan

    2017-12-01

    Full Text Available As an important starting point for optimizing the structure of agricultural products and implementing green production methods, the direction of orchard management development is directly related to the success of “supply side” reform in the fruit industry in China. However, in the context of the progressive aging of the rural labor force, is the old labor force still capable of the high labor intensity and fine cultivation management needed, such as for pruning, or to maintain or improve the application efficiency of fertilizers? In this paper, based on the micro-production data of peach farmers in Jiangsu Province, we explore the influence of aging on the management of fruit trees and further introduce fruit tree management into the production function to analyze the effects of different orchard management methods on fertilizer efficiency. The results show that with the increase of labor force age, although the total labor investment of aged farmer households has somewhat increased, significant differences exist in the distribution of labor investment between the different production processes due to the different labor demands from the various production processes. In technical stages that demand good physical capabilities, such as pruning and flower/fruit thinning, elderly farmers have significantly reduced labor investment than younger ones, and this relative shortfall further reduces the marginal output of their chemical and organic fertilizers. Foreseeably, the aging of the rural labor force will have a negative impact on the efficiency of chemical and other fertilizers, cost-cutting, and profit-making in the fruit and nut industries, which have the same management methods for pruning and flower (fruit thinning. Therefore, this paper offers relevant policy recommendations for the optimization of production tools, expansion of operation scale, and development of socialized services for the fruit industry, etc.

  10. Differential metabolite profiles during fruit development in high-yielding oil palm mesocarp.

    Directory of Open Access Journals (Sweden)

    Huey Fang Teh

    Full Text Available To better understand lipid biosynthesis in oil palm mesocarp, in particular the differences in gene regulation leading to and including de novo fatty acid biosynthesis, a multi-platform metabolomics technology was used to profile mesocarp metabolites during six critical stages of fruit development in comparatively high- and low-yielding oil palm populations. Significantly higher amino acid levels preceding lipid biosynthesis and nucleosides during lipid biosynthesis were observed in a higher yielding commercial palm population. Levels of metabolites involved in glycolysis revealed interesting divergence of flux towards glycerol-3-phosphate, while carbon utilization differences in the TCA cycle were proven by an increase in malic acid/citric acid ratio. Apart from insights into the regulation of enhanced lipid production in oil palm, these results provide potentially useful metabolite yield markers and genes of interest for use in breeding programmes.

  11. Identification of microRNA targets in tomato fruit development using high-throughput sequencing and degradome analysis

    NARCIS (Netherlands)

    Karlova, R.B.; Haarst, van J.C.; Maliepaard, C.A.; Geest, van de H.C.; Bovy, A.G.; Lammers, M.; Angenent, G.C.; Maagd, de R.A.

    2013-01-01

    MicroRNAs (miRNAs) play important roles in plant development through regulation of gene expression by mRNA degradation or translational inhibition. Despite the fact that tomato (Solanum lycopersicum) is the model system for studying fleshy fruit development and ripening, only a few experimentally

  12. Using Intervention Mapping for Systematic Development of Two School-Based Interventions Aimed at Increasing Children's Fruit and Vegetable Intake

    Science.gov (United States)

    Reinaerts, E.; De Nooijer, J.; De Vries, N. K.

    2008-01-01

    Purpose: The purpose of this paper is to show how the intervention mapping (IM) protocol could be applied to the development of two school-based interventions. It provides an extensive description of the development, implementation and evaluation of two interventions which aimed to increase fruit and vegetable (F&V) consumption among primary…

  13. Development of saw palmetto (Serenoa repens) fruit and extract standard reference materials.

    Science.gov (United States)

    Schantz, Michele M; Bedner, Mary; Long, Stephen E; Molloy, John L; Murphy, Karen E; Porter, Barbara J; Putzbach, Karsten; Rimmer, Catherine A; Sander, Lane C; Sharpless, Katherine E; Thomas, Jeanice B; Wise, Stephen A; Wood, Laura J; Yen, James H; Yarita, Takashi; NguyenPho, Agnes; Sorenson, Wendy R; Betz, Joseph M

    2008-10-01

    As part of a collaboration with the National Institutes of Health's Office of Dietary Supplements and the Food and Drug Administration's Center for Drug Evaluation and Research, the National Institute of Standards and Technology has developed two standard reference materials (SRMs) representing different forms of saw palmetto (Serenoa repens), SRM 3250 Serenoa repens fruit and SRM 3251 Serenoa repens extract. Both of these SRMs have been characterized for their fatty acid and phytosterol content. The fatty acid concentration values are based on results from gas chromatography with flame ionization detection (GC-FID) and mass spectrometry (GC/MS) analysis while the sterol concentration values are based on results from GC-FID and liquid chromatography with mass spectrometry analysis. In addition, SRM 3250 has been characterized for lead content, and SRM 3251 has been characterized for the content of beta-carotene and tocopherols. SRM 3250 (fruit) has certified concentration values for three phytosterols, 14 fatty acids as triglycerides, and lead along with reference concentration values for four fatty acids as triglycerides and 16 free fatty acids. SRM 3251 (extract) has certified concentration values for three phytosterols, 17 fatty acids as triglycerides, beta-carotene, and gamma-tocopherol along with reference concentration values for three fatty acids as triglycerides, 17 fatty acids as free fatty acids, beta-carotene isomers, and delta-tocopherol and information values for two phytosterols. These SRMs will complement other reference materials currently available with concentrations for similar analytes and are part of a series of SRMs being developed for dietary supplements.

  14. Kisspeptin regulates ovarian steroidogenesis during delayed embryonic development in the fruit bat, Cynopterus sphinx.

    Science.gov (United States)

    Anuradha; Krishna, Amitabh

    2017-11-01

    Cynopterus sphinx, a fruit bat, undergoes delayed embryonic development during the winter months, a period that corresponds to low levels of progesterone and estradiol synthesis by the ovary. Kisspeptins (KPs) are a group of neuropeptide hormones that act via G-protein coupled receptor 54 (GPR54) to stimulate hypothalamic secretion of Gonadotropin-releasing hormone, thereby regulating ovarian steroidogenesis, folliculogenesis, and ovulation. GPR54 is also expressed in the ovary, suggesting a direct role for KPs in ovarian steroidogenesis. The aim of present study was to determine if a low serum level of KP is responsible for reduced progesterone and estradiol levels during the period of delayed embryonic development in C. sphinx. Indeed, low serum KP abundance corresponded to reduced expression of GPR54 in ovarian luteal cells during the period of delayed development compared to normal development. In vitro and in vivo treatment with KP increased GPR54 abundance, via Extracellular signal regulated kinase and its downstream mediators, leading to increased progesterone synthesis in the ovary during delayed embryonic development. KP treatment also increased cholesterol uptake and elevated expression of Luteinizing hormone receptor and Steroid acute regulatory protein in the ovary, suggesting that elevation in circulating KP during delayed embryonic development may reactivate luteal activity. KPs may also enhance cell survival (BCL-2, reduced Caspase 3 activity) and angiogenesis (Vascular endothelium growth factor) during this period. The findings of this study thus demonstrate a regulatory role for KPs in the maintenance of luteal steroidogenesis during pregnancy in C. sphinx. © 2017 Wiley Periodicals, Inc.

  15. Identification of 2,5-dimethyl-4-hydroxy-3[2H]-furanone beta-D-glucuronide as the major metabolite of a strawberry flavour constituent in humans.

    Science.gov (United States)

    Roscher, R; Koch, H; Herderich, M; Schreier, P; Schwab, W

    1997-08-01

    2,5-Dimethyl-4-hydroxy-3[2H]furanone (Furaneol, DMHF) [3658-77-3], an important flavour constituent of strawberry fruit, was administered to four male and two female volunteers using fresh strawberries as a natural DMHF source. The amount excreted was determined by measuring urinary levels of DMHF and DMHF glucuronide. DMHF glucuronide was synthesized and the structure elucidated by mens of 1H, 13C and two dimensional nuclear magnetic resonance, as well as mass spectral data. Identification and quantification of DMHF glucuronide in human urine were achieved after solid phase extraction on XAD-2 using reverse-phase reverse-phase HPLC with either on-line UV/VIS or electrospray tandem mass spectrometry detection. Male and female volunteers excreted 59-69% and 81-94%, respectively, of the DMHF dose (total of free and glycosidically bound DMHF in strawberries) as DMHF glucuronide in urine within 24 hr. The amount of DMHF excretion was independent of the dose size and the ratio of free to glycosidically bound forms of DMHF in strawberry fruit. DMHF, DMHF glucoside and its 6'-O-malonyl derivative, naturally occurring in strawberries, were not detected in human urine.

  16. Modelling central metabolic fluxes by constraint-based optimization reveals metabolic reprogramming of developing Solanum lycopersicum (tomato) fruit.

    Science.gov (United States)

    Colombié, Sophie; Nazaret, Christine; Bénard, Camille; Biais, Benoît; Mengin, Virginie; Solé, Marion; Fouillen, Laëtitia; Dieuaide-Noubhani, Martine; Mazat, Jean-Pierre; Beauvoit, Bertrand; Gibon, Yves

    2015-01-01

    Modelling of metabolic networks is a powerful tool to analyse the behaviour of developing plant organs, including fruits. Guided by our current understanding of heterotrophic metabolism of plant cells, a medium-scale stoichiometric model, including the balance of co-factors and energy, was constructed in order to describe metabolic shifts that occur through the nine sequential stages of Solanum lycopersicum (tomato) fruit development. The measured concentrations of the main biomass components and the accumulated metabolites in the pericarp, determined at each stage, were fitted in order to calculate, by derivation, the corresponding external fluxes. They were used as constraints to solve the model by minimizing the internal fluxes. The distribution of the calculated fluxes of central metabolism were then analysed and compared with known metabolic behaviours. For instance, the partition of the main metabolic pathways (glycolysis, pentose phosphate pathway, etc.) was relevant throughout fruit development. We also predicted a valid import of carbon and nitrogen by the fruit, as well as a consistent CO2 release. Interestingly, the energetic balance indicates that excess ATP is dissipated just before the onset of ripening, supporting the concept of the climacteric crisis. Finally, the apparent contradiction between calculated fluxes with low values compared with measured enzyme capacities suggest a complex reprogramming of the metabolic machinery during fruit development. With a powerful set of experimental data and an accurate definition of the metabolic system, this work provides important insight into the metabolic and physiological requirements of the developing tomato fruits. © 2014 The Authors The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.

  17. Induction of materials for mutation breeding of strawberry (FragariaxAnanassa) by gamma irradiation (Phase 2)

    International Nuclear Information System (INIS)

    Le Tien Thanh; Huynh Thi Trung; Pham Van Nhi; Vu Thi Trac

    2016-01-01

    In this study, New Zaeland strawberry runners was propagated in vitro to create clump of buds for Gamma irradiation. The experimental result showed that LD_5_0 was 52 Gy. Basing on the LD_5_0, we selected the 5 doses of 20, 40, 60, 80 and 100 Gy to irradiate in vitro materials for creating the potential mutants. Irradiated materials were propagated continuously in vitro to complete 300 in vitro plants per dose. On farm, ex vitro plants were planted on the spout (from the ground) by hydroponic method with the number of 200 plants per dose. Some mutant characteristics increased gradually toward the increasing of gamma doses as dwarf plants (in the dose of 60 Gy, 80 Gy and 100 Gy), plants had small fruits (in the dose of 60 Gy, 80 Gy, 100 Gy), plants had deformed fruits (in the dose of 20 Gy, 40 Gy, 60 Gy, 80 Gy and 100 Gy). In this study, we selected four mutants with 2 mutation fruits were changed to fruit heart-shape with symbol DT 1 (dose 60 Gy) and DT 2 (dose 80 Gy); 2 mutants were dwarf plants, the ungrown bud, wrinkled leaf, deformed fruits, high sweetness with symbol DN 1 (dose 60 Gy) and DN 2 (dose 80 Gy). (author)

  18. The pro children intervention: applying the intervention mapping protocol to develop a school-based fruit and vegetable promotion programme.

    Science.gov (United States)

    Pérez-Rodrigo, Carmen; Wind, Marianne; Hildonen, Christina; Bjelland, Mona; Aranceta, Javier; Klepp, Knut-Inge; Brug, Johannes

    2005-01-01

    The importance of careful theory-based intervention planning is recognized for fruit and vegetable promotion. This paper describes the application of the Intervention Mapping (IM) protocol to develop the Pro Children intervention to promote consumption of fruit and vegetable among 10- to 13-year-old schoolchildren. Based on a needs assessment, promotion of intake of fruit and vegetable was split into performance objectives and related personal, social and environmental determinants. Crossing the performance objectives with related important and changeable determinants resulted in a matrix of learning and change objectives for which appropriate educational strategies were identified. Theoretically similar but culturally relevant interventions were designed, implemented and evaluated in Norway, the Netherlands and Spain during 2 school years. Programme activities included provision of fruits and vegetables in the schools, guided classroom activities, computer-tailored feedback and advice for children, and activities to be completed at home with the family. Additionally, optional intervention components for community reinforcement included incorporation of mass media, school health services or grocery stores. School project committees were supported. The Pro Children intervention was carefully developed based on the IM protocol that resulted in a comprehensive school-based fruit and vegetable promotion programme, but culturally sensible and locally relevant. (c) 2005 S. Karger AG, Basel

  19. Screening and identification of putative allergens in berry fruits of the Rosaceae family: technical challenges.

    Science.gov (United States)

    Marzban, Gorji; Maghuly, Fatemeh; Herndl, Anita; Katinger, Hermann; Laimer, Margit

    2008-01-01

    Cross-reactive proteins in small fruits of the Rosaceae family like strawberry, raspberry and blackberry revealed an unexpected complex IgE-reactivity pattern. Several copies of PR-10 and PR-14 proteins were detected by Southern blots in strawberry, raspberry and blackberry. In raspberry, the highest similarity at the DNA level for PR-10 and PR-14 (Rub i 1 and Rub i 3) was detected to strawberry sequences of Fra a 1 and Fra a 3. At the protein level, Rub i 1 and Rub i 3 showed more than 70% identity with homologous proteins of rosaceous fruits. Furthermore, raspberries contained additional putative allergens, e.g. class III acidic chitinases and cyclophilins. Blackberries were shown to share at least two well-known major fruit allergens with other rosaceous fruits, namely PR-10s and PR-14s homologous proteins. However the IgE-reactive proteins of small fruits are still not extensively investigated. The main challenges in studying small fruit allergens are the complexity of the fruit matrix, the diversity of physico-chemical properties of fruit proteins, the lack of appropriate protein extraction procedures and the missing information about the influence of processing treatments on food components.

  20. Rehydration properties of hybrid method dried fruit enriched by natural components

    Science.gov (United States)

    Kowalska, Hanna; Marzec, Agata; Kowalska, Jolanta; Ciurzyńska, Agnieszka; Samborska, Kinga; Bialik, Michał; Lenart, Andrzej

    2018-04-01

    The aim of the study was to determine the impact of osmotic pre-dehydration and drying of fruit on the rehydration properties of dried fruit. Herein, the effect of fruit juice, applied as a natural enriching substance was very important. In addition, the properties of dried fruits obtained through combined air-drying and subsequent microwave-vacuum drying with `puffing' effect were similar to the freeze-dried fruits, but showed other rehydration properties. As raw material, frozen strawberry (Honeoye variety) and fresh apples (Idared variety) were used in the study. The apples and partially defrosted strawberries were prior dehydrated in solutions of sucrose and a mixture of sucrose with chokeberry juice concentrate at 50°C for 2 h. Next, the fruit samples were dried by one of two ways: air-drying (50°C, 5 h) and microwavevacuum drying for about 360 s; and freeze-drying (30°C, 63 Pa, 24 h). The rehydration was carried out in distilled water (20°C, 5 h). The osmotic pre-dehydration hindered fruit drying process. The impact of drying method became particularly evident while examining the kinetics of rehydration. During the rehydration of the pre-dehydrated dried fruit a slower hydration could be observed. Freeze-dried strawberries absorbed 2-3 times more water than those dried by the `puffing' effect.

  1. Comprehensive analysis of NAC transcription factors and their expression during cucumber fruit spine development

    Science.gov (United States)

    Cucumber (Cucumis sativus L.) is an important vegetable crop worldwide, and fruit trichomes ir spines are an important trait for external fruit quality. The mechanisms underlying spine formation are not well understood, but there is evidence of the involvement of the plant-specific NAC family of tra...

  2. QTL mapping in multiple populations and development stages reveals dynamic quantitative trait loci for fruit size in cucumbers of different market classes.

    Science.gov (United States)

    Weng, Yiqun; Colle, Marivi; Wang, Yuhui; Yang, Luming; Rubinstein, Mor; Sherman, Amir; Ophir, Ron; Grumet, Rebecca

    2015-09-01

    QTL analysis in multi-development stages with different QTL models identified 12 consensus QTLs underlying fruit elongation and radial growth presenting a dynamic view of genetic control of cucumber fruit development. Fruit size is an important quality trait in cucumber (Cucumis sativus L.) of different market classes. However, the genetic and molecular basis of fruit size variations in cucumber is not well understood. In this study, we conducted QTL mapping of fruit size in cucumber using F2, F2-derived F3 families and recombinant inbred lines (RILs) from a cross between two inbred lines Gy14 (North American picking cucumber) and 9930 (North China fresh market cucumber). Phenotypic data of fruit length and diameter were collected at three development stages (anthesis, immature and mature fruits) in six environments over 4 years. QTL analysis was performed with three QTL models including composite interval mapping (CIM), Bayesian interval mapping (BIM), and multiple QTL mapping (MQM). Twenty-nine consistent and distinct QTLs were detected for nine traits from multiple mapping populations and QTL models. Synthesis of information from available fruit size QTLs allowed establishment of 12 consensus QTLs underlying fruit elongation and radial growth, which presented a dynamic view of genetic control of cucumber fruit development. Results from this study highlighted the benefits of QTL analysis with multiple QTL models and different mapping populations in improving the power of QTL detection. Discussion was presented in the context of domestication and diversifying selection of fruit length and diameter, marker-assisted selection of fruit size, as well as identification of candidate genes for fruit size QTLs in cucumber.

  3. Cloning, Expression, and Characterization of Sorbitol Transporters from Developing Sour Cherry Fruit and Leaf Sink Tissues1

    Science.gov (United States)

    Gao, Zhifang; Maurousset, Laurence; Lemoine, Remi; Yoo, Sang-Dong; van Nocker, Steven; Loescher, Wayne

    2003-01-01

    The acyclic polyol sorbitol is a primary photosynthetic product and the principal photosynthetic transport substance in many economically important members of the family Rosaceace (e.g. almond [Prunus dulcis (P. Mill.) D.A. Webber], apple [Malus pumila P. Mill.], cherry [Prunus spp.], peach [Prunus persica L. Batsch], and pear [Pyrus communis]). To understand key steps in long-distance transport and particularly partitioning and accumulation of sorbitol in sink tissues, we have cloned two sorbitol transporter genes (PcSOT1 and PcSOT2) from sour cherry (Prunus cerasus) fruit tissues that accumulate large quantities of sorbitol. Sorbitol uptake activities and other characteristics were measured by heterologous expression of PcSOT1 and PcSOT2 in yeast (Saccharomyces cerevisiae). Both genes encode proton-dependent, sorbitol-specific transporters with similar affinities (Km sorbitol of 0.81 mm for PcSOT1 and 0.64 mm for PcSOT2). Analyses of gene expression of these transporters, however, suggest different roles during leaf and fruit development. PcSOT1 is expressed throughout fruit development, but especially when growth and sorbitol accumulation rates are highest. In leaves, PcSOT1 expression is highest in young, expanding tissues, but substantially less in mature leaves. In contrast, PcSOT2 is mainly expressed only early in fruit development and not in leaves. Compositional analyses suggest that transport mediated by PcSOT1 and PcSOT2 plays a major role in sorbitol and dry matter accumulation in sour cherry fruits. Presence of these transporters and the high fruit sorbitol concentrations suggest that there is an apoplastic step during phloem unloading and accumulation in these sink tissues. Expression of PcSOT1 in young leaves before completion of the transition from sink to source is further evidence for a role in determining sink activity. PMID:12692316

  4. [Development of a didactic questionnaire to measure vegetable and fruit intake during the school recess. Pilot test].

    Science.gov (United States)

    Medina, Catalina; Villanueva-Borbolla, Montserrat; Barquera, Simón

    2012-10-01

    To develop an open, self-reported questionnaire, focused in understanding intake and intention to increase vegetable and fruit consumption during the Mexican children's lunchtime. After assessing pilot tests, the self-reported questionnaire was applied as a classroom exercise to measure children's consumptions of fruit and vegetables. A total of 486 children completed the questionnaire (49.8% males and 50.2% females). The reported food consumption indicates that 24.5% of children do not consume vegetables or fruits, and among consumers the most common portion size is one. Girls consume more vegetables and fruits and they can recognize more benefits. A total of 9.7% of the participants that do not consume VF, expressed less intention of including vegetables or fruits in their lunch. These results were similar to those reported by other authors. This study is useful to identify strengths and limitations during the design and implementation of this type of instrument to understand children's food habits during school recess.

  5. Polyphenols and Volatiles in Fruits of Two Sour Cherry Cultivars, Some Berry Fruits and Their Jams

    Directory of Open Access Journals (Sweden)

    Branka Levaj

    2010-01-01

    Full Text Available This paper reports about the content of polyphenols and volatiles in fresh fruits of two sour cherry cultivars (Marasca and Oblačinska, some berry fruits (strawberry Maya, raspberry Willamette and wild blueberry and the corresponding low sugar jams. Phenolic compounds (hydroxybenzoic and hydroxycinnamic acids, flavan 3-ols and flavonols were determined by high-performance liquid chromatography (HPLC. Those found in the fruits were also found in the jams. Jams contained lower amounts of polyphenols than fresh fuits, but their overall retention in jams was relatively high. Among fruits, sour cherry Marasca had the highest level of polyphenols, while sour cherry Marasca jam and raspberry Willamette jam had the highest level of polyphenols among jams. The major flavonoid in all investigated fruits, except in sour cherry Oblačinska, was (–-epicatechin. Sour cherry Marasca had the highest level of (–-epicatechin (95.75 mg/kg, and it also contained very high amounts of flavonols, derivatives of quercetin and kaempferol. Hydroxybenzoic acids (HBAs were not found in sour cherries Marasca and Oblačinska, but were found in berry fruits and jams. Phenolic compound (+-gallocatechin was found only in Marasca fruit and jam. Ellagic acid was found in the highest concentration in raspberry Willamette fruit and jam. Hydroxycinnamic acids (HCAs were found in all the investigated fruits, with the exception of a derivative of ferulic acid, which was not found in strawberry. Derivatives of caffeic, p-coumaric and chlorogenic acids were found in all the investigated fruits, with chlorogenic acid being the most abundant, especially in sour cherry Marasca. Volatiles were determined by gas chromatography (GC and expressed as the peak area of the identified compounds. All investigated volatiles of fresh fruit were also determined in the related jams with relatively high retention. Sour cherries Marasca and Oblačinska contained the same volatile compounds, but

  6. Characterization and quantification of flavonoids and organic acids over fruit development in American cranberry (Vaccinium macrocarpon) cultivars using HPLC and APCI-MS/MS.

    Science.gov (United States)

    Wang, Yifei; Johnson-Cicalese, Jennifer; Singh, Ajay P; Vorsa, Nicholi

    2017-09-01

    Cranberry flavonoids, including anthocyanins, flavonol glycosides and proanthocyanidins, and organic acids were characterized and quantified by HPLC and LC-MS/MS during fruit development and ripening in eight cranberry cultivars. Anthocyanin biosynthesis initiated at early fruit development and reached highest level in mature fruit, with significant differences between cultivars. Major flavonol glycosides, including the most abundant quercetin-3-galactoside and myricetin-3-galactoside, showed consistent concentrations during the season with moderate fluctuation, and were at similar levels in mature fruits of the eight cultivars. Proanthocyanidins declined during fruit development and then increased slightly in later maturation stages. Levels of various proanthocyanidin oligomers/polymers with different degree-of-polymerization were highly correlated within a cultivar during fruit development. Cultivars with coancestry exhibited similar levels (high/low) of anthocyanins or proanthocyanidins, indicating genetic effects on biosynthesis of such flavonoids. All cultivars showed similar levels of malic and citric acids, and declining levels of quinic acid during fruit development. Benzoic acid was extremely low early in the season and increased sharply during fruit ripening. Levels of quinic and citric acids were significantly different among cultivars in the mature fruit. Concentrations of proanthocyanidins, anthocyanins, quinic acid and benzoic acid have a strong developmental association in developing ovaries. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Solidago canadensis L essential oil vapor effectively inhibits Botrytis cinerea growth and preserves postharvest quality of strawberry as a food model system

    OpenAIRE

    Shumin Liu; Xingfeng Shao; Yanzhen Wei; Yonghua Li; Feng Xu; Hongfei Wang

    2016-01-01

    This study investigated the anti-fungal properties of Solidago canadensis L essential oil (SCLEO) against Botrytis cinerea in vitro, and its ability to control gray mold and maintain quality in strawberry fruits. SCLEO exhibited dose-dependent antifungal activity against B. cinerea and profoundly altered mycelial morphology, cellular ultrastructure, and membrane permeability as evaluated by scanning electron microscopy, transmission electron microscopy, and fluorescence microscopy. SCLEO vapo...

  8. Solidago canadensis L. Essential Oil Vapor Effectively Inhibits Botrytis cinerea Growth and Preserves Postharvest Quality of Strawberry as a Food Model System

    OpenAIRE

    Liu, Shumin; Shao, Xingfeng; Wei, Yanzhen; Li, Yonghua; Xu, Feng; Wang, Hongfei

    2016-01-01

    This study investigated the anti-fungal properties of Solidago canadensis L. essential oil (SCLEO) against Botrytis cinerea in vitro, and its ability to control gray mold and maintain quality in strawberry fruits. SCLEO exhibited dose-dependent antifungal activity against B. cinerea and profoundly altered mycelial morphology, cellular ultrastructure, and membrane permeability as evaluated by scanning electron microscopy, transmission electron microscopy, and fluorescence microscopy. SCLEO vap...

  9. New developments in fruit and vegetables consumption in the period 1999-2004 in Denmark - a quantile regression approach

    DEFF Research Database (Denmark)

    Hansen, Aslak Hedemann

    2008-01-01

    The development in the consumption of fruit and vegetables in the period 1999-2004 in Denmark was investigated using quantile regression and two previously overlooked problems were identified. First, the change in the ten percent quantile samples decreased. This could have been caused by changes ...

  10. How Is a Fruit Tree Like You? Using Artistic Metaphors to Explore and Develop Emotional Competence in Children

    Science.gov (United States)

    Geldard, Kathryn; Foo, Rebecca Yin; Shakespeare-Finch, Jane

    2009-01-01

    Counselling children often requires the use of supplementary strategies in order to interest and engage the child in the therapeutic process. One such strategy is the Metaphorical Fruit Tree (MFT); an art metaphor suited to exploring and developing self-concept. Quantitative and qualitative data was used to explore the relationships between…

  11. The development of the fruit-body of Marasmius cornelii (Agaricales) and of a new species of Marasmius sect. Gloiocephala

    NARCIS (Netherlands)

    Corner, E.J.H.

    1992-01-01

    The gymnocarpic development of the very small and gill-less fruit-body of Marasmius cornelii is directed by intrinsic factors until the lengthening stem becomes geotropic. The primordium is covered from a very early stage by a hymenioderm of clavate encrusted cells with a few lanceolate

  12. Biosynthesis, accumulation and degradation of theobromine in developing Theobroma cacao fruits.

    Science.gov (United States)

    Zheng, Xin-Qiang; Koyama, Yoko; Nagai, Chifumi; Ashihara, Hiroshi

    2004-04-01

    We have studied the purine alkaloid content and purine metabolism in Theobroma cacao fruits at differing growth stages: Stage A (young small fruit, fresh weight, ca. 2 g); stage B (medium size fruit, fresh weight, ca. 100 g) and stage C (large size, fresh weight, ca. 500 g). The major purine alkaloid in stage A fruits (mainly pericarp) was theobromine (0.7 micromol g(-1) fresh weight), followed by caffeine (0.09 micromol g(-1) fresh weight). The theobromine content of the pericarp decreased sharply with tissue age, and the caffeine content decreased gradually. A large amount of theobromine (22 micromol g(-1) fresh weight) had accumulated in seeds (mainly cotyledons) of stage C fruits. Theobromine was found also in the seed coat and placenta. Tracer experiments with [8-(14)C]adenine show that the major sites of theobromine synthesis are the young pericarp and cotyledons of T. cacao fruits. Limited amounts of purine alkaloids may be transported from the pericarp to seed tissue, but most purine alkaloids that accumulated in seeds appeared to be synthesised in cotyledons. Degradation of [8-(14)C]theobromine and [8-(14)C]caffeine to CO2 via 3-methylxanthine and ureides (allantoin and allantoic acid) was detected only in the pericarp of stage C fruits.

  13. Model Development of Cold Chains for Fresh Fruits and Vegetables Distribution: A Case Study in Bali Province

    Science.gov (United States)

    Waisnawa, I. N. G. S.; Santosa, I. D. M. C.; Sunu, I. P. W.; Wirajati, IGAB

    2018-01-01

    In developing countries such as Indonesia, as much as 40% of total vegetables and fruits production becomes waste because of lack refrigeration. This condition also contributes a food crisis problem besides other factor such as, climate change and number of population. Cold chain system that will be modelled in this study is for vegetables and fruits and refrigeration system as the main devices. In future, this system will play an important role for the food crisis solution where fresh food can be distributed very well with significant low waste. The fresh food also can be kept with good quality and hygienist (bacteria contaminated). Cold Chain model will be designed using refrigeration components including, pre cooling chiller, cold room, and truck refrigeration. This study will be conducted by survey and observation di around Bali Province focus on vegetables and fruits production center. Interviews and questionnaire will be also done to get some information about the conventional distribution obstacles and problem. Distribution mapping will be developed and created. The data base of the storage characteristic of the fruits and vegetable also collected through experiment and secondary data. Depend on the mapping and data base can be developed a cold chain model that has the best performance application. The model will be can directly apply in Bali to get eligible cold chain in Bali. The cold chain model will be compared with the conventional distribution system using ALCC/LCC method and also others factor and will be weighted to get better results.

  14. Identification and characterization of novel microRNAs for fruit development and quality in hot pepper (Capsicum annuum L.).

    Science.gov (United States)

    Liu, Zhoubin; Zhang, Yuping; Ou, Lijun; Kang, Linyu; Liu, Yuhua; Lv, Junheng; Wei, Ge; Yang, Bozhi; Yang, Sha; Chen, Wenchao; Dai, Xiongze; Li, Xuefeng; Zhou, Shudong; Zhang, Zhuqing; Ma, Yanqing; Zou, Xuexiao

    2017-04-15

    MicroRNAs (miRNAs) are non-coding small RNAs which play an important regulatory role in various biological processes. Previous studies have reported that miRNAs are involved in fruit development in model plants. However, the miRNAs related to fruit development and quality in hot pepper (Capsicum annuum L.) remains unknown. In this study, small RNA populations from different fruit ripening stages and different varieties were compared using next-generation sequencing technology. Totally, 59 known miRNAs and 310 novel miRNAs were identified from four libraries using miRDeep2 software. For these novel miRNAs, 656 targets were predicted and 402 of them were annotated. GO analysis and KEGG pathways suggested that some of the predicted miRNAs targeted genes involved in starch sucrose metabolism and amino sugar as well as nucleotide sugar metabolism. Quantitative RT-PCR validated the contrasting expression patterns between several miRNAs and their target genes. These results will provide an important foundation for future studies on the regulation of miRNAs involved in fruit development and quality. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Study on the growth and development of brinjal shoot and fruit borer ...

    African Journals Online (AJOL)

    Jane

    2011-09-28

    Sep 28, 2011 ... 2Department of Crop Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. ... various aspects of the insect's life and activity with a view to evolving .... of brinjal shoot and fruit borer is still a difficult job. Many.

  16. Research and development of Camellia oleifera fruit sheller and sorting machine

    Science.gov (United States)

    Kang, Di; Wang, Yong; Fan, Youhua; Chen, Zejun

    2018-01-01

    Camellia oleifera fruit sheller in this paper was designed by the principle of kneading and extruding. This machine adopted the rolling classification sieve to screen camellia oleifera fruit with different sizes into the husking device, and camellia oleifera fruit was shelled in the mutually co-operative action of transport belt and flexible rubbing washboard. After research, in the condition that the moisture content of camellia oleifera fruit was below 55%, the vibration of the motor frequency was 50 Hz and the horizontal angle of sorting belt was 50 degrees∼55 degrees, the processing capacity was more than 900 kg/h, the threshing ratio was more than 97%, the seed broken ratio was less than 5%, the loss ratio was less than 1%. The machine is of great value in actual production, and should be widely spread and applied.

  17. De novo comparative transcriptome analysis of genes involved in fruit morphology of pumpkin cultivars with extreme size difference and development of EST-SSR markers.

    Science.gov (United States)

    Xanthopoulou, Aliki; Ganopoulos, Ioannis; Psomopoulos, Fotis; Manioudaki, Maria; Moysiadis, Theodoros; Kapazoglou, Aliki; Osathanunkul, Maslin; Michailidou, Sofia; Kalivas, Apostolos; Tsaftaris, Athanasios; Nianiou-Obeidat, Irini; Madesis, Panagiotis

    2017-07-30

    The genetic basis of fruit size and shape was investigated for the first time in Cucurbita species and genetic loci associated with fruit morphology have been identified. Although extensive genomic resources are available at present for tomato (Solanum lycopersicum), cucumber (Cucumis sativus), melon (Cucumis melo) and watermelon (Citrullus lanatus), genomic databases for Cucurbita species are limited. Recently, our group reported the generation of pumpkin (Cucurbita pepo) transcriptome databases from two contrasting cultivars with extreme fruit sizes. In the current study we used these databases to perform comparative transcriptome analysis in order to identify genes with potential roles in fruit morphology and fruit size. Differential Gene Expression (DGE) analysis between cv. 'Munchkin' (small-fruit) and cv. 'Big Moose' (large-fruit) revealed a variety of candidate genes associated with fruit morphology with significant differences in gene expression between the two cultivars. In addition, we have set the framework for generating EST-SSR markers, which discriminate different C. pepo cultivars and show transferability to related Cucurbitaceae species. The results of the present study will contribute to both further understanding the molecular mechanisms regulating fruit morphology and furthermore identifying the factors that determine fruit size. Moreover, they may lead to the development of molecular marker tools for selecting genotypes with desired morphological traits. Copyright © 2017. Published by Elsevier B.V.

  18. Isolation and characterization of Enterobacteriaceae species infesting post-harvest strawberries and their biological control using bacteriophages.

    Science.gov (United States)

    Kurtböke, D Ipek; Palk, A; Marker, A; Neuman, C; Moss, L; Streeter, K; Katouli, M

    2016-10-01

    Strawberry is a significantly consumed fruit worldwide, mostly without being subjected to disinfection processes. During the harvest and transfer from farm to consumers as well as where organic farming practises have been employed, the surface of the fruit may become contaminated by pathogenic bacteria. Post-harvest strawberry fruits in punnets available for public consumption were thus screened for the presence of enteric bacteria in the Sunshine Coast region of Queensland, Australia. Some of the tested samples (13 %) were found to carry such bacteria and even in greater numbers if organic amendments were used (69 %). The bacteria were found to belong in the genera of Escherichia, Enterobacter, Raoultella, Klebsiella, Pantoea, Shigella, Citrobacter and Cronobacter within the family Enterobacteriaceae. Some of the isolates were found to adhere to Caco-2 cells representing human gut epithelium as well as carrying virulence and toxin genes. Resistance mostly against sulphafurazole, cefoxitin, ampicillin and nitrofurantoin was found among 14 different antimicrobial agents tested including 100 % resistance to cefoxitin and ampicillin in the genus Pantoea. In the second phase of the study, bacteriophages were isolated against the isolates and were subsequently applied to post-harvest fruits. A significant (P ≤ 0.001) reduction in the number of enteric bacteria was observed when a high-titre polyvalent bacteriophage suspension (×10(12) PFU/mL) was applied to the fruit surface. Bacteriophages also decreased the adhesion of the Escherichia coli isolates to Caco-2 cells. Findings might indicate that biological control using bacteriophages might be of significant value for the industry targeting to reduce pathogenic loads of bacteria on the fruit.

  19. Prunasin hydrolases localization during fruit development in sweet and bitter almonds

    DEFF Research Database (Denmark)

    Sánchez Pérez, Raquel; Belmonte, Fara Sáez; Borch-Jensen, Jonas

    2012-01-01

    Amygdalin is a cyanogenic diglucoside and constitutes the bitter component in bitter almond (Prunus dulcis). Amygdalin concentration increases in the course of fruit formation. The monoglucoside prunasin is the precursor of amygdalin. Prunasin may be degraded to hydrogen cyanide, glucose, and ben......Amygdalin is a cyanogenic diglucoside and constitutes the bitter component in bitter almond (Prunus dulcis). Amygdalin concentration increases in the course of fruit formation. The monoglucoside prunasin is the precursor of amygdalin. Prunasin may be degraded to hydrogen cyanide, glucose...

  20. G * E interaction for growth, yield and quality characters of strawberry (fragaria ananassa duch.) under bangladesh conditions

    International Nuclear Information System (INIS)

    Rahman, M.M.; Rahman, M.M.

    2015-01-01

    A multi location trial with five promising strawberry genotypes viz., Sweet Charlie, Festival, Camarosa, FA 008 and BARI Strawberry-1 was conducted under different sub tropical climatic conditions of Bangladesh to study their G * E interaction. Location were Gazipur, Rajshahi and Chittagong during the period from August 2009 to May 2011. Combined analysis of variance indicated significant variation among the genotypes as well as location for almost all the characters studied. Genotype environment studies indicated different response of genotypes over locations for most of the characters suggesting location wise environmental variation. Stability parameter revealed that a particular genotype did not perform consistently for all the characters. The genotype Festival considered as stable for most of the characters except days to flowering and ascorbic acid content of fruits, and considered as stable over different locations. For this reason, the genotype .Festival. could be well thought-out and less receptive to the environmental conditions and might be recommended for all environments. On the other hand, environmental indices indicated that the environment of Rajshahi was found to be most favourable for strawberry cultivation followed by that of Gazipur and Chittagong. (author)

  1. Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening.

    Science.gov (United States)

    Zhu, Qianglong; Gao, Peng; Liu, Shi; Zhu, Zicheng; Amanullah, Sikandar; Davis, Angela R; Luan, Feishi

    2017-01-03

    Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an economically important crop with an attractive ripe fruit that has colorful flesh. Fruit ripening is a complex, genetically programmed process. In this study, a comparative transcriptome analysis was performed to identify the regulators and pathways that are involved in the fruit ripening of pale-yellow-flesh cultivated watermelon (COS) and red-flesh cultivated watermelon (LSW177). We first identified 797 novel genes to extend the available reference gene set. Second, 3958 genes in COS and 3503 genes in LSW177 showed at least two-fold variation in expression, and a large number of these differentially expressed genes (DEGs) during fruit ripening were related to carotenoid biosynthesis, plant hormone pathways, and sugar and cell wall metabolism. Third, we noted a correlation between ripening-associated transcripts and metabolites and the key function of these metabolic pathways during fruit ripening. The results revealed several ripening-associated actions and provide novel insights into the molecular mechanisms underlying the regulation of watermelon fruit ripening.

  2. A loop-mediated isothermal amplification assay and sample preparation procedure for sensitive detection of Xanthomonas fragariae in strawberry

    Science.gov (United States)

    Xanthomonas fragariae is a bacterium that causes angular leaf spot of strawberry. Asymptomatic infections are common and contribute to the difficulties in disease management. The aim of this study was to develop a loop-mediated isothermal amplification (LAMP) assay with a bacterial enrichment proced...

  3. Strawberries Do Grow on Trees

    Science.gov (United States)

    Milne, Ian

    2004-01-01

    An important aim of primary science programmes should be to continue to encourage and nurture children in creating their own explanations. As Osborne and Freyberg (1985) contended, the aims of primary science in the early years should start with the children developing explanations for their experiences. The first two of their six developing aims…

  4. Development of female fruit fly, Ceratitis species, attractant systems for trapping and sterility assessment in South Africa

    International Nuclear Information System (INIS)

    Eyles, D.K.; Du Plessis, N.; Barnes, B.N.

    1999-01-01

    Fruit in the Western Cape Province of South Africa is attacked by the Mediterranean fruit fly, Ceratitis capitata, and the Natal fruit fly, C. rosa. Control costs and crop losses amount to an estimated US$ 5 million per year. The ARC-Fruit, Vine and Wine Research Institute in Stellenbosch has been taking part in the FAO/IAEA Co-ordinated Research Programme (CRP) to develop a female attractant systems for C. capitata during the 1996/97 and 1997/98 growing seasons. These seasons represented Year 3 and Year 4, respectively, of the female attractant CRP. The performance of these attractant systems in monitoring C. rosa was also evaluated. The Year 3 trial was carried out in a plum orchard with a low fruit fly population, and the Year 4 trial was carried out in the same plum orchard, as well as in a pear orchard with a high fruit fly population. Four female trapping systems were evaluated in Year 3 and five in Year 4. In Year 3, two systems consisted of a locally-manufactured buckettype 'Sensus' trap containing one of two different female attractants (β-caryophyllene, or β-caryophyllene + protein hydrolysate), one was a McPhail trap (IPMT) baited with borax + protein hydrolysate, and the fourth was an open bottom dry trap (OBDT) baited with the three component ammonium acetate, putrescine and trimethylamine system (FA-3). Sensus traps and Jackson traps baited with the male fruit fly attractant Trimedlure (TML) were also included. In Year 4, there was one wet and one dry IPMT system (both baited with FA-3), one IPMT system (borax + protein hydrolysate), and one wet and one dry Tephri trap system (both with FA-3). Jackson traps baited with TML were also included. Results from Year 3 showed that of the systems tested, the best female C. capitata attractant systems under low population conditions were the IPMT system baited with borax + protein hydrolysate, and the OBDT system baited with FA-3. β-caryophyllene in a Sensus trap did not adequately attract female C

  5. Firmness at Harvest Impacts Postharvest Fruit Softening and Internal Browning Development in Mechanically Damaged and Non-damaged Highbush Blueberries (Vaccinium corymbosum L.).

    Science.gov (United States)

    Moggia, Claudia; Graell, Jordi; Lara, Isabel; González, Guillermina; Lobos, Gustavo A

    2017-01-01

    Fresh blueberries are very susceptible to mechanical damage, which limits postharvest life and firmness. Softening and susceptibility of cultivars "Duke" and "Brigitta" to developing internal browning (IB) after mechanical impact and subsequent storage was evaluated during a 2-year study (2011/2012, 2012/2013). On each season fruit were carefully hand-picked, segregated into soft (<1.60 N), medium (1.61-1.80 N), and firm (1.81-2.00 N) categories, and then either were dropped (32 cm) onto a hard plastic surface or remained non-dropped. All fruit were kept under refrigerated storage (0°C and 85-88% relative humidity) to assess firmness loss and IB after 7, 14, 21, 28, and 35 days. In general, regardless of cultivar or season, high variability in fruit firmness was observed within each commercial harvest, and significant differences in IB and softening rates were found. "Duke" exhibited high softening rates, as well as high and significant r 2 between firmness and IB, but little differences for dropped vs. non-dropped fruit. "Brigitta," having lesser firmness rates, exhibited almost no relationships between firmness and IB (especially for non-dropped fruit), but marked differences between dropping treatments. Firmness loss and IB development were related to firmness at harvest, soft and firm fruit being the most and least damaged, respectively. Soft fruit were characterized by greater IB development during storage along with high soluble solids/acid ratio, which could be used together with firmness to estimate harvest date and storage potential of fruit. Results of this work suggest that the differences in fruit quality traits at harvest could be related to the time that fruit stay on the plant after turning blue, soft fruit being more advanced in maturity. Finally, the observed differences between segregated categories reinforce the importance of analyzing fruit condition for each sorted group separately.

  6. Integrated options for the management of black root rot of strawberry caused by Rhizoctonia solani Kuhn.

    Science.gov (United States)

    Asad-Uz-Zaman, Md; Bhuiyan, Mohammad Rejwan; Khan, Mohammad Ashik Iqbal; Alam Bhuiyan, Md Khurshed; Latif, Mohammad Abdul

    2015-02-01

    An investigation was made to manage strawberry black root rot caused by Rhizoctonia solani (R. solani) through the integration of Trichoderma harzianum (T. harzianum) isolate STA7, mustard oil cake and Provax 200. A series of preliminary experiments were conducted to select a virulent isolate of R. solani, an effective isolate of T. harzianum, a suitable organic amendment, and a suitable fungicide before setting the experiment for integration. The pathogenicity of the selected four isolates of R. solani was evaluated against strawberry and isolate SR1 was selected as the test pathogen due to its highest virulent (95.47% mortality) characteristics. Among the 20 isolates of T. harzianum, isolate STA7 showed maximum inhibition (71.97%) against the test pathogen (R. solani). Among the fungicides, Provax-200 was found to be more effective at lowest concentration (100 ppm) and highly compatible with Trichoderma isolates STA7. In the case of organic amendments, maximum inhibition (59.66%) of R. solani was obtained through mustard oil cake at the highest concentration (3%), which was significantly superior to other amendments. Minimum percentages of diseased roots were obtained with pathogen (R. solani)+Trichoderma+mustard oil cake+Provax-200 treatment, while the highest was observed with healthy seedlings with a pathogen-inoculated soil. In the case of leaf and fruit rot diseases, significantly lowest infected leaves as well as fruit rot were observed with a pathogen+Trichoderma+mustard oil cake+Provax-200 treatment in comparison with the control. A similar trend of high effectiveness was observed by the integration of Trichoderma, fungicide and organic amendments in controlling root rot and fruit diseases of strawberry. Single application of Trichoderma isolate STA7, Provax 200 or mustard oil cake did not show satisfactory performance in terms of disease-free plants, but when they were applied in combination, the number of healthy plants increased significantly. The

  7. Systematic analysis and comparison of the PHD-Finger gene family in Chinese pear (Pyrus bretschneideri) and its role in fruit development.

    Science.gov (United States)

    Cao, Yunpeng; Han, Yahui; Meng, Dandan; Abdullah, Muhammad; Li, Dahui; Jin, Qing; Lin, Yi; Cai, Yongping

    2018-04-20

    PHD-finger proteins, which belongs to the type of zinc finger family, and that play an important role in the regulation of both transcription and the chromatin state in eukaryotes. Currently, PHD-finger proteins have been well studied in animals, while few studies have been carried out on their function in plants. In the present study, 129 non-redundant PHD-finger genes were identified from 5 Rosaceae species (pear, apple, strawberry, mei, and peach); among them, 31 genes were identified in pear. Subsequently, we carried out a bioinformatics analysis of the PHD-finger genes. Thirty-one PbPHD genes were divided into 7 subfamilies based on the phylogenetic analysis, which are consistent with the intron-exon and conserved motif analyses. In addition, we identified five segmental duplication events, implying that the segmental duplications might be a crucial role in the expansion of the PHD-finger gene family in pear. The microsynteny analysis of five Rosaceae species showed that there were independent duplication events in addition to the genome-wide duplication of the pear genome. Subsequently, ten expressed PHD-finger genes of pear fruit were identified using qRT-PCR, and one of these genes, PbPHD10, was identified as an important candidate gene for the regulation of lignin synthesis. Our research provides useful information for the further analysis of the function of PHD-finger gene family in pear.

  8. ORGANOLEPTIC QUALITY OF FRUIT SORBETS CONTAINING YACON (SMALLANTHUS SONCHIFOLIUS Poepp. and Endl.

    Directory of Open Access Journals (Sweden)

    Kinga Topolska

    2015-02-01

    Full Text Available Due to the growing consumer interest in functional food, sorbets containing yacon root powder as a source of many valuable compounds, especially fructans, may be a desirable alternative to traditional ice cream desserts. The aim of this work was to evaluate organoleptic quality (taste, color, aroma as well as consistency and structure of fruit sorbets containing Smallanthus sonchifolius (Poepp. and Endl. root powder in dependence on kind of fruit (orange, cherry, strawberry, and its share in sorbet’s recipe, using a 9-point hedonic scale (1 point =„I do not like it at all“, 9 = „I like it very much“. The results showed that organoleptic quality was depended on the kind and the share of fruit used to the sorbet production. Strawberry sorbets gained over 80% of maximal scores. One can conclude that yacon root powder can be used as the ingredient of fruit sorbet, with increased consumer liking.

  9. Elemental characterisation of strawberry grown in Islamabad by k0-instrumental neutron activation analysis and atomic absorption spectrophotometry and its dietary assessment

    International Nuclear Information System (INIS)

    Mohammad Wasim; Nasir Khalid; Mohammad Arif; Jamshed Hussain Zaidi

    2012-01-01

    Mineral contents of strawberry, collected from different farms of Islamabad were analysed by semi-absolute k 0-instrumental neutron activation analysis and atomic absorption spectrophotometry. The samples were irradiated at two research reactors located in Pakistan Institute of Nuclear Science and Technology (PINSTECH), Islamabad. The analytical methodologies were validated by analysing reference materials, IAEA-336 (lichen) and IAEA-V-10 (hay powder). In all the samples, a total of 26 elements were quantified, among them 16 elements (Ca, Cd, Cl, Co, Cr, Cs, Fe, K, Mg, Mn, Na, Pb, Ru, Sc, Sr and Zn) were found in all the samples. The determined elemental concentrations in strawberry were compared with the reported values from other countries. In comparison with the mineral contents of other fruits, strawberry stands best source of Mn and the second most important source of K after banana. Intake of trace metals through this source was calculated and it was found that strawberry provides Mn (1.95-3.68 mg/kg), Cr (19.2-46.3 x 10 -3 mg/kg), Fe (3.45-8.72 mg/kg), K (1,520-1,670 mg/kg) and Mg (100-220 mg/kg), which forms 26, 19, 14, 7 and 7% of the recommended dietary allowances for the respective metals. The daily intake of Cd and Pb were compared with the provisional tolerable weekly intake defined by FAO/WHO. (author)

  10. Screening of the presence organophosphates and organochlorines pesticide residues in vegetables and fruits using gas chromatography-mass spectrometry

    Science.gov (United States)

    Putri, Dillani; Aryana, Nurhani; Aristiawan, Yosi; Styarini, Dyah

    2017-01-01

    Pesticides is commonly used to improve the quality of agricultural product, especially in vegetables and fruits. Due to pesticide residues in the product become a concern to consumer health, monitoring and analysis of pesticide residues in agriculture product need to be established. The certified reference material (CRM) is often benefited to obtain accurate results in analysis. It is required as the quality control to improve quality assurance of the testing results. Unfortunately in Indonesia, the development of matrix CRM for the analysis of pesticide residues in vegetables and fruits is still limited. This study is aimed to determine the type of commodity and target analyte to be employed in the development of CRM for pesticides in vegetables and fruits. As the preliminary study, the screening of 11 commodities of fresh vegetables and fruits has been conducted to review the information about the presence of organophosphates (OPs) and organochlorines (OCs) in the sample. In this analysis, QuEChERS technique was used in the extraction process and the qualitative analysis was evaluated by using GC-MS. The results showed that strawberry and celery contain residues of pesticide chlorpyrifos. Further analysis of the commodity celery from seven different places has been conducted, resulting that from 3 of all 7 samples (43%) were positive containing chlorpyrifos. Therefore, the development of CRM for chlorpyrifos in celery will be our next research project.

  11. Application of carboxymethyl cellulose and chitosan coatings containing Mentha spicata essential oil in fresh strawberries.

    Science.gov (United States)

    Shahbazi, Yasser

    2018-06-01

    The aim of the present study was to investigate the effects of carboxymethyl cellulose (CMC) and chitosan (CH) coatings containing Mentha spicata essential oil (MSO 0.1 and 0.2%) on survival of Listeria monocytogenes, and physicochemical (weight loss, titratable acidity and pH), microbial (total viable count, psychrotrophic bacteria as well as yeasts and molds) and sensory (appearance, color, texture and overall acceptability) properties of fresh strawberries during refrigerated storage. The treatments of fruits with CH+MSO 0.2% and CMC+MSO 0.2% resulted in the best microbial, physicochemical and organoleptic properties after 12days storage. The final population of L. monocytogenes in treated samples was decreased by 3.92-3.69 compared to control groups. It can be concluded that CH and CMC coatings enriched with MSO can be used as appropriate active packaging materials to preserve fresh strawberries in the food industry. Copyright © 2018 Elsevier B.V. All rights reserved.

  12. Detection of Colletotrichum acutatum Latent Infections in Strawberry Petioles and Leaves

    Directory of Open Access Journals (Sweden)

    Nataša Duduk

    2008-01-01

    Full Text Available Colletotrichum acutatum is the most significant agent of anthracnose strawberry fruit rot. Besides being a necrotrophic pest, it can spend a part of its life cycle as an epiphyte, in a form of latent infection. The presence of the fungi on symptomless plant tissue is considered one of the main ways of distribution of this economically harmful pathogen in the world. Investigation of latent C. acutatum infection was carried out on artificially inoculated strawberries. The initiation of fungi sporulation on symptomless petioles and leaves was carried out by exposing them to the herbicide paraquat (0.25% and low temperatures, which caused plant tissue decay in different ways. Surface sterilization with 0.5% NaOCl precedes the exposure of plant material to paraquat. The freezing procedure was carried out by exposure of plant material to the temperature of -20°C for 2h. After the freezing, one group was rinsed in Tween 20 (18 μl/l, and another group underwent surface sterilization in 0.0525% NaOCl with an addition of Tween 20 (18 μl/l. After 6 days of incubation, the appearance of acervuli and conidia was detected in 93.33 to 100% plant parts exposed to paraquat treatment and freezing procedure. In inoculated parts which were not exposed to herbicides or low temperatures, the presence of acervuli was detected in 3.33% tested petioles and 6.67% leaves.

  13. Methodology for determination of benzimidazolic fungicides residues in strawberry and lettuce by HPLC-DAD

    International Nuclear Information System (INIS)

    Dangond Araujo, Jose Jairo; Guerrero dallos, Jairo Arturo

    2006-01-01

    systemic fungicides like benzimidazolic compounds are used to protect several crops of fruits and vegetables. in this work a new method for analysis of Benomyl, carbendazim and thiabendazol in strawberry and lettuce by high performance liquid chromatography with diode array detector (HPLC-DAD) was validated. benomyl residues were determined after its conversion to carbendazim. pesticide residues were extracted from strawberry and lettuce samples with ethyl acetate and these extracts were cleaned up by gel permeation chromatography (GPC). final determination was carried out by HPLC-DAD in reverse phase column. the method is selective, specific, precise and accurate. the calibration curves show linearity over concentration range of 1.24 to 6.19 mg/kg, with detection limits of 0.40 and 0.27 mg/kg and quantification limits of 1.35 and 0.81 mg/kg for carbendazim and thiabendazole respectively. the recovery experiments yielding averages of 90 %. n o residues of these compounds were found in collected samples from specific areas of Cundinamarca, Colombia

  14. Improving Storability of Strawberries with Gaseous Chlorine Dioxide in Perforated Clamshell Packaging

    Directory of Open Access Journals (Sweden)

    Chiabrando Valentina

    2018-06-01

    Full Text Available A chlorine dioxide generating pad has been used as postharvest treatment to maintain the quality and safety of strawberries in two different storage conditions. During the short storage time (3 days at 4°C + 2 days at 20°C fruit treated with ClO2 maintained better quality parameters, as color, titratable acidity, total soluble solids, anthocyanins, antioxidant capacity and lower values of weight loss. During the long storage time (12 days at 2°C, the parameters related to postharvest quality were generally preserved and the effect of ClO2 was positive to reduce the total yeast and mold, except the last period of the experiment when the ClO2 gas-generating pad was probably exhausted. The sensory evaluation revealed that the sanitization with ClO2 maintained a global positive acceptance in particular during the short storage time. The results suggest that this treatment may be suitable to maintain the quality during short storage and long storage until 8 days and it may be an important alternative sanitizer thanks to the positive action against the yeast and mold without modifying the quality of the strawberries.

  15. Organochlorine pesticide residues in strawberries from integrated pest management and organic farming.

    Science.gov (United States)

    Fernandes, Virginia C; Domingues, Valentina F; Mateus, Nuno; Delerue-Matos, Cristina

    2011-07-27

    A rapid, specific, and sensitive method based on the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) method and a cleanup using dispersive solid-phase extraction with MgSO(4), PSA, and C18 sorbents has been developed for the routine analysis of 14 pesticides in strawberries. The analyses were performed by three different analytical methodologies: gas chromatography (GC) with electron capture detection (ECD), mass spectrometry (MS), and tandem mass spectrometry (MS/MS). The recoveries for all the pesticides studied were from 46 to 128%, with relative standard deviation of residue limits (MRL) accepted in Portugal for organochlorine pesticides (OCP). A survey study of strawberries produced in Portugal in the years 2009-2010 obtained from organic farming (OF) and integrated pest management (IPM) was developed. Lindane and β-endosulfan were detected above the MRL in OF and IPM. Other OCP (aldrin, o,p'-DDT and their metabolites, and methoxychlor) were found below the MRL. The OCP residues detected decreased from 2009 to 2010. The QuEChERS method was successfully applied to the analysis of strawberry samples.

  16. Wing morphology and flight development in the short-nosed fruit bat Cynopterus sphinx.

    Science.gov (United States)

    Elangovan, Vadamalai; Yuvana Satya Priya, Elangovan; Raghuram, Hanumanth; Marimuthu, Ganapathy

    2007-01-01

    Postnatal changes in wing morphology, flight development and aerodynamics were studied in captive free-flying short-nosed fruit bats, Cynopterus sphinx. Pups were reluctant to move until 25 days of age and started fluttering at the mean age of 40 days. The wingspan and wing area increased linearly until 45 days of age by which time the young bats exhibited clumsy flight with gentle turns. At birth, C. sphinx had less-developed handwings compared to armwings; however, the handwing developed faster than the armwing during the postnatal period. Young bats achieved sustained flight at 55 days of age. Wing loading decreased linearly until 35 days of age and thereafter increased to a maximum of 12.82 Nm(-2) at 125 days of age. The logistic equation fitted the postnatal changes in wingspan and wing area better than the Gompertz and von Bertalanffy equations. The predicted minimum power speed (V(mp)) and maximum range speed (V(mr)) decreased until the onset of flight and thereafter the V(mp) and V(mr) increased linearly and approached 96.2% and 96.4%, respectively, of the speed of postpartum females at the age of 125 days. The requirement of minimum flight power (P(mp)) and maximum range power (P(mr)) increased until 85 days of age and thereafter stabilised. The minimum theoretical radius of banked turn (r(min)) decreased until 35 days of age and thereafter increased linearly and attained 86.5% of the r(min) of postpartum females at the age of 125 days.

  17. Fitness, Competitive Ability, and Mutation Stability of Isolates of Colletotrichum acutatum from Strawberry Resistant to QoI Fungicides.

    Science.gov (United States)

    Forcelini, Bruna B; Rebello, Carolina S; Wang, Nan-Yi; Peres, Natalia A

    2018-04-01

    Quinone-outside inhibitor (QoI) fungicides are used to manage anthracnose of strawberry, caused by Colletotrichum acutatum. However, selection for resistance to QoI fungicides was first reported in 2013 in Florida and, subsequently, in strawberry nurseries and production areas across the United States and Canada. C. acutatum resistance to QoIs is associated with the G143A point mutation in the cytochrome b gene. This mutation is known to be associated with field resistance even at high rates of QoI. In this study, we investigated the relative fitness and competitive ability of QoI-resistant and -sensitive C. acutatum isolates. A fitness comparison did not indicate any difference between resistant and sensitive isolates in aggressiveness, spore production, and mycelial growth at different temperatures. Additionally, in the absence of selection pressure, resistant and sensitive isolates were equally competitive. Cultivation of QoI-resistant and QoI-sensitive isolates for four culture cycles in vitro in the absence of azoxystrobin showed that QoI resistance was stable. The observed lack of fitness penalties and stability of the G143A mutation in QoI-resistant C. acutatum populations suggest that the interruption and further reintroduction of QoI fungicides might not be an option for strawberry nurseries and fruit production areas. Further investigation of alternative chemical and nonchemical C. acutatum control practices, in addition to the integration of multisite fungicides, is needed to reduce the occurrence and distribution of QoI-resistant populations in strawberry fields.

  18. Transcriptome analysis and its application in identifying genes associated with fruiting body development in basidiomycete Hypsizygus marmoreus.

    Directory of Open Access Journals (Sweden)

    Jinjing Zhang

    Full Text Available To elucidate the mechanisms of fruit body development in H. marmoreus, a total of 43609521 high-quality RNA-seq reads were obtained from four developmental stages, including the mycelial knot (H-M, mycelial pigmentation (H-V, primordium (H-P and fruiting body (H-F stages. These reads were assembled to obtain 40568 unigenes with an average length of 1074 bp. A total of 26800 (66.06% unigenes were annotated and analyzed with the Kyoto Encyclopedia of Genes and Genomes (KEGG, Gene Ontology (GO, and Eukaryotic Orthologous Group (KOG databases. Differentially expressed genes (DEGs from the four transcriptomes were analyzed. The KEGG enrichment analysis revealed that the mycelium pigmentation stage was associated with the MAPK, cAMP, and blue light signal transduction pathways. In addition, expression of the two-component system members changed with the transition from H-M to H-V, suggesting that light affected the expression of genes related to fruit body initiation in H. marmoreus. During the transition from H-V to H-P, stress signals associated with MAPK, cAMP and ROS signals might be the most important inducers. Our data suggested that nitrogen starvation might be one of the most important factors in promoting fruit body maturation, and nitrogen metabolism and mTOR signaling pathway were associated with this process. In addition, 30 genes of interest were analyzed by quantitative real-time PCR to verify their expression profiles at the four developmental stages. This study advances our understanding of the molecular mechanism of fruiting body development in H. marmoreus by identifying a wealth of new genes that may play important roles in mushroom morphogenesis.

  19. Metabolic Fingerprinting to Assess the Impact of Salinity on Carotenoid Content in Developing Tomato Fruits

    Directory of Open Access Journals (Sweden)

    Lieven Van Meulebroek

    2016-05-01

    Full Text Available As the presence of health-promoting substances has become a significant aspect of tomato fruit appreciation, this study investigated nutrient solution salinity as a tool to enhance carotenoid accumulation in cherry tomato fruit (Solanum lycopersicum L. cv. Juanita. Hereby, a key objective was to uncover the underlying mechanisms of carotenoid metabolism, moving away from typical black box research strategies. To this end, a greenhouse experiment with five salinity treatments (ranging from 2.0 to 5.0 decisiemens (dS m−1 was carried out and a metabolomic fingerprinting approach was applied to obtain valuable insights on the complicated interactions between salinity treatments, environmental conditions, and the plant’s genetic background. Hereby, several hundreds of metabolites were attributed a role in the plant’s salinity response (at the fruit level, whereby the overall impact turned out to be highly depending on the developmental stage. In addition, 46 of these metabolites embraced a dual significance as they were ascribed a prominent role in carotenoid metabolism as well. Based on the specific mediating actions of the retained metabolites, it could be determined that altered salinity had only marginal potential to enhance carotenoid accumulation in the concerned tomato fruit cultivar. This study invigorates the usefulness of metabolomics in modern agriculture, for instance in modeling tomato fruit quality. Moreover, the metabolome changes that were caused by the different salinity levels may enclose valuable information towards other salinity-related plant processes as well.

  20. Prospects for the development of disease-resistant temperate fruit plants by mutation induction

    International Nuclear Information System (INIS)

    Campbell, A.I.; Wilson, D.

    1977-01-01

    In most of the present conventional fruit breeding programmes disease resistance has become an important objective. Progress is slow because of the long generation time and the genetic complexity of most tree fruit species. The complexity is such that cultivars can only be maintained as clones and it is unlikely that identical genotypes could ever be sexually produced. Hence, the prospect of changing a few characters in an otherwise unchanged genetic background, as might be done by somatic mutation, is attractive. The occurence of natural mutations in some fruit cultivars and the induction of mutations in others demonstrates that such an approach is possible for some characters at least and these may include disease resistance. The yet limited success of mutation breeding in fruit crops may be due in part to the innate difficulties with this group of plants but may also be a consequence of the faulty methods that have been used in the past. New techniques of inducing and selecting mutants in fruit trees are reported, with particular reference to disease resistance and some basic guidelines for success are suggested. The type of disease resistance required will undoubtedly affect the approach used. In theory, monogenic resistance seems more likely to respond to change by mutation induction than polygenic resistance. However, the multiple effects seen in the natural spur-type apple mutants and in the preliminary results with induced apple mutations at Long Ashton suggest that field resistance to some major diseases may not be an unreasonable target

  1. Investigation of fruit irradiation: bibliographical review; Investigacion de irradiacion de frutas: revision bibliografica

    Energy Technology Data Exchange (ETDEWEB)

    Luna C, P C

    1991-01-15

    It was carried out a bibliographical review that embraces the years 1984-1987, on the relating works to the irradiation of some fruits like the apple, date, peach, plum, cherry, papaya, grape, banana, pear and strawberry. The purpose is to have a reference on the doses and the conditions used by several investigators for some fruits, as for its disinfestation and extension of shelf life. (Author)

  2. Nitrogen levels in the cultivation of strawberries in soilless culture Doses de nitrogênio no cultivo fora do solo do morangueiro

    Directory of Open Access Journals (Sweden)

    Jerônimo L Andriolo

    2011-12-01

    Full Text Available In soilless grown strawberry crops, the nitrogen concentration of the nutrient solution affects plant growth and fruit yield and quality. The present research was conducted to determine the effect of nitrogen concentration in the nutrient solution on plant growth and development and fruit yield and quality of this crop. Treatments consisted of five nutrient solutions at nitrogen concentrations of 6.5 (T1, 8.0 (T2, 9.5 (T3, 11.0 (T4 and 12.5 (T5 mmol L-1, in an entirely randomised experimental design with four replications. Ripe fruit yield was determined during the harvest period from June 6th to November 27th, 2009. Number of leaves, shoot and root dry mass and crown diameter were determined at the later date. Number of leaves, shoot and root dry mass and crown diameter decreased by effect of increasing N concentrations in the nutrient solution. Fruit yield and fruit size fitted a polynomial model, with maximum values at 8.9 mmol N L-1. The N concentration used for the strawberry crop in soilless growing systems can be reduced to 8.9 mmol L-1 without any reduction in fruit yield.No cultivo sem solo do morangueiro, a concentração de nitrogênio da solução nutritiva afeta o crescimento da planta, a produtividade e a qualidade das frutas. O objetivo do trabalho foi determinar o efeito da concentração de nitrogênio da solução nutritiva no crescimento e desenvolvimento da planta e produção de frutas do morangueiro. Os tratamentos foram constituídos por cinco concentrações de N na solução nutritiva: 6,5 (T1, 8,0 (T2, 9,5 (T3, 11,0 (T4 e 12,5 (T5 mmol L-1, em delineamento experimental inteiramente casualizado, com quatro repetições. Foi determinada a produção de frutas no período entre 6 de junho e 27 de novembro de 2009, o número de folhas e a massa seca da parte aérea, coroas e raízes na última data. O número de folhas, a massa seca da parte aérea e das raízes e o diâmetro da coroa diminuíram com o aumento da concentra

  3. Epidemiology and Control of Strawberry Bacterial Angular Leaf Spot Disease Caused by Xanthomonas fragariae

    Directory of Open Access Journals (Sweden)

    Da-Ran Kim

    2016-08-01

    Full Text Available Strawberry bacterial angular leaf spot (ALS disease, caused by Xanthomonas fragariae has become increasingly problematic in the strawberry agro-industry. ALS causes small angular water-soaked lesions to develop on the abaxial leaf surface. Studies reported optimum temperature conditions for X. fragariae are 20°C and the pathogen suffers mortality above 32°C. However, at the nursery stage, disease symptoms have been observed under high temperature conditions. In the present study, results showed X. fragariae transmission was via infected maternal plants, precipitation, and sprinkler irrigation systems. Systemic infections were detected using X. fragariae specific primers 245A/B and 295A/B, where 300-bp and 615-bp were respectively amplified. During the nursery stage (from May to August, the pathogen was PCR detected only in maternal plants, but not in soil or irrigation water through the nursery stage. During the cultivation period, from September to March, the pathogen was detected in maternal plants, progeny, and soil, but not in water. Additionally, un-infected plants, when planted with infected plants were positive for X. fragariae via PCR at the late cultivation stage. Chemical control for X. fragariae with oxolinic acid showed 87% control effects against the disease during the nursery period, in contrast to validamycin-A, which exhibited increased efficacy against the disease during the cultivation stage (control effect 95%. To our knowledge, this is the first epidemiological study of X. fragariae in Korean strawberry fields.

  4. Surface survival and internalization of salmonella through natural cracks on developing cantaloupe fruits, alone or in the presence of the melon wilt pathogen Erwinia tracheiphila.

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    Dhiraj Gautam

    Full Text Available Outbreaks of foodborne illness attributed to the consumption of Salmonella-tainted cantaloupe have occurred repeatedly, but understanding of the ecology of Salmonella on cantaloupe fruit surfaces is limited. We investigated the interactions between Salmonella enterica Poona, the plant pathogenic bacterium Erwinia tracheiphila, and cantaloupe fruit. Fruit surfaces were inoculated at the natural cracking stage by spreading S. enterica and E. tracheiphila, 20 µl at 107 cfu/ml, independently or together, over a 2×2 cm rind area containing a crack. Microbial and microscopic analyses were performed at 0, 9 and 24 days post inoculation (DPI. Even at 24 DPI (fruit maturity S. enterica was detected on 14% and 40% of the fruit inoculated with S. enterica alone and the two-pathogen mixture, respectively. However, the population of S. enterica declined gradually after initial inoculation. E. tracheiphila, inoculated alone or together with Salmonella, caused watersoaked lesions on cantaloupe fruit; but we could not conclude in this study that S. enterica survival on the fruit surface was enhanced by the presence of those lesions. Of fruit inoculated with E. tracheiphila alone and sampled at 24 DPI, 61% had watersoaked lesions on the surface. In nearly half of those symptomatic fruits the watersoaking extended into the sub-rind mesocarp, and E. tracheiphila was recovered from that tissue in 50% of the symptomatic fruit. In this work, E. tracheiphila internalized through natural cracks on developing fruits. S. enterica was never detected in the fruit interior (ca. 2-3 mm below rind surface under the limited conditions of our experiments, but the possibility that it, or other human pathogens that contaminate fresh produce, might also do so should be investigated under a wider range of conditions and produce types.

  5. Revised Distribution of Bactrocera tryoni in Eastern Australia and Effect on Possible Incursions of Mediterranean Fruit Fly: Development of Australia's Eastern Trading Block.

    Science.gov (United States)

    Dominiak, Bernard C; Mapson, Richard

    2017-12-05

    Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), commonly called 'Queensland fruit fly' in Australia, and Mediterranean fruit fly (Ceratitis capitata Wiedemann) (Diptera: Tephritidae) are the two most economically important fruit fly in Australia with B. tryoni in the east and Mediterranean fruit fly in the west. The two species coexisted for several decades, but it is believed that B. tryoni displaced Mediterranean fruit fly. In southeastern Australia, this was deemed inadequate for export market access, and a large fruit fly free zone (fruit fly exclusion zone) was developed in 1996 where B. tryoni was eradicated by each state department in their portion of the zone. This zone caused an artificial restricted distribution of B. tryoni. When the fruit fly exclusion zone was withdrawn in Victoria and New South Wales in 2013, B. tryoni became endemic once again in this area and the national distribution of B. tryoni changed. For export markets, B. tryoni is now deemed endemic to all eastern Australian states, except for the Greater Sunraysia Pest-Free Area. All regulatory controls have been removed between eastern states, except for some small zones, subject to domestic market access requirements. The eastern Australian states now form a B. tryoni endemic trading group or block. All Australian states and territories maintain legislation to regulate the movement of potentially infested host fruit into their states. In particular, eastern states remain active and regulate the entry of commodities possibly infested with Mediterranean fruit fly. The combination of regulatory controls limits the chances of Mediterranean fruit fly entering eastern states, and if it did, Mediterranean fruit fly is unlikely to establish in the opposition to a well-established B. tryoni population. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  6. Carotenogenic gene expression and carotenoid accumulation in three varieties of Cucurbita pepo during fruit development.

    Science.gov (United States)

    Obrero, Ángeles; González-Verdejo, Clara I; Die, Jose V; Gómez, Pedro; Del Río-Celestino, Mercedes; Román, Belén

    2013-07-03

    The control of gene expression is a crucial regulatory mechanism in carotenoid accumulation of fruits and flowers. We investigated the role of transcriptional regulation of nine genes involved in the carotenoid biosynthesis pathway in three varieties of Cucurbita pepo with evident differences in fruit color. The transcriptional levels of the key genes involved in the carotenoid biosynthesis were higher in flower-, leaf-, and fruit skin tissues than flesh tissues. This correlated with higher concentration of carotenoid content in these tissues. The differential expression among the colored and white cultivars detected for some genes, such as LCYe, in combination with other regulatory mechanisms, could explain the large differences found in terms of carotenoid content among the three varieties. These results are a first step to elucidate carotenogenesis in C. pepo and demonstrate that, in general, regulation of the pathway genes is a critical factor that determines the accumulation of these compounds.

  7. Financial results achieved in short-day strawberry production

    Directory of Open Access Journals (Sweden)

    Galić Dragan

    2014-01-01

    Full Text Available In South-western Ontario's continental climate (short days, hot summers and very cold winters the matted-row system was the dominant production system to grow short-day strawberries. Varieties-staggered production (planting a combination of early, mid and late-season varieties provides strawberry harvest from five to seven weeks. Short-day strawberries are vegetative grown in the first year, and harvested for two consecutive years. The total cost of short-day strawberry production was 54,370 $CAD/ha. The production and harvest costs in the first and second years were 20,812 $CAD/ha and 16,930 $CAD/ ha, respectively, and accounted for 69.42% of the total. Pre-plans operations were the least expensive procedures costing 8.13%, while planting and care of young plants made up 22.45% of the total costs. The total income of growing short-day strawberries under a matted-row system was 76,671 $CAD/ha (the first and second production years 41,330 $CAD/ha and 35,341 $CAD/ha, respectively. The short-day strawberries in matted-row system, with average yield of 15,722 kg/ha, generated a net revenue of 22,300 $CAD/ha.

  8. Promising sour cherry hybrids (Prunus cerasus L. developed at Fruit Research Institute Čačak

    Directory of Open Access Journals (Sweden)

    Radičević Sanja

    2010-01-01

    Full Text Available At Fruit Research Institute in Čačak, major objectives of the work on breeding new sour cherry (Prunus cerasus L. cultivars are high cropping, large, high-quality fruits and resistance to causal agents of diseases and pests. As a result of the planned hybridization, more than 10,000 hybrid seedlings have been developed from about 40 cultivars within more than 110 parental combinations, among which are 'Čačanski rubin' ('Shasse Morello' x 'Köröser Weichsel' and 'Šumadinka' ('Köröser Weichsel' x 'Heimanns Konserven Weichsel' which have been named and released. Ten-year study of 11 hybrids, selected from the population of about 3,000 hybrid seedlings, gave four hybrids which have been singled out as elite (III/23, III/31, II/40 i XII/57. These hybrids are currently under procedure of being released as new cultivars. The paper presents two-year results of the study of ripening time, pomological properties, biochemical composition of fruits, and field resistance to causal agents of diseases and pests attacking the above named genotypes which were compared to standard cultivar 'Heimanns Konserven Weichsel'. In the studied hybrids, fruit weight, soluble solids content and sugars content were higher than in standard cultivar. In addition, they exhibit substantial field resistance to causal agents of brown rot (Monilinia laxa /Ader et Ruhl./ Honey ex Whetz., cherry leaf spot (Blumeriella jaapii (Rehm. v. Arx., shot-hole (Clasterosporium carpophilum (Lév. Aderh. and cherry fruit fly (Rhagoletis cerasi L. attack.

  9. Altered Fruit and Seed Development of Transgenic Rapeseed (Brassica napus Over-Expressing MicroRNA394.

    Directory of Open Access Journals (Sweden)

    Jian Bo Song

    Full Text Available Fruit and seed development in plants is a complex biological process mainly involved in input and biosynthesis of many storage compounds such as proteins and oils. Although the basic biochemical pathways for production of the storage metabolites in plants are well characterized, their regulatory mechanisms are not fully understood. In this study, we functionally identified rapeseed (Brassica napus miR394 with its target gene Brassica napus leaf curling responsiveness (BnLCR to dissect a role of miR394 during the fruit and seed development. Transgenic rapeseed plants over-expressing miR394 under the control of the cauliflower mosaic virus 35S promoter were generated. miR394 over-expression plants exhibited a delayed flowering time and enlarged size of plants, leaf blade, pods and seed body, but developed seeds with higher contents of protein and glucosinolates (GLS and lower levels of oil accumulation as compared to wild-type. Over-expression of miR394 altered the fatty acid (FA composition by increasing several FA species such as C16:0 and C18:0 and unsaturated species of C20:1 and C22:1 but lowering C18:3. This change was accompanied by induction of genes coding for transcription factors of FA synthesis including leafy cotyledon1 (BnLEC1, BnLEC2, and FUSCA3 (FUS3. Because the phytohormone auxin plays a crucial role in fruit development and seed patterning, the DR5-GUS reporter was used for monitoring the auxin response in Arabidopsis siliques and demonstrated that the DR5 gene was strongly expressed. These results suggest that BnmiR394 is involved in rapeseed fruit and seed development.

  10. Effect of molybdenum on yield and nutrient content of the strawberry

    International Nuclear Information System (INIS)

    Bender, R.J.; Barros, I.B.I. de

    1984-01-01

    The cultivation of strawberries (Fragaria x ananassa Duch.) cv. Campinas in nutrient solution using five fields levels of molybdenum (MO), 0.010 ppm, 0.010 ppm, 0.10 ppm, 1.0 ppm and control (Without applying molybdenum). The experiment was carried out in greenhouse and phytothron to evaluate the changes in the mineral status of the leaves and the quality of the berries. The concentration of Mo in the leaves, at the beginning of fruiting stage, varied from 0.5 ppm to 64 ppm. The total N in the levels was affected significantly by Mo. Average weight of the berries were affected by treatments and presented a quadratic regression response. (M.A.C.) [pt

  11. Desenvolvimento do fruto da lichieira (Litchi chinensis Sonn. 'Bengal' Fruit development in 'Bengal' litchi (Litchi chinensis Sonn.

    Directory of Open Access Journals (Sweden)

    Luiz Carlos Chamhum Salomão

    2006-04-01

    Full Text Available Estudou-se o desenvolvimento do fruto da lichieira (Litchi chinensis Sonn. 'Bengal'. Realizaram-se amostragens semanais, com início aos 35 dias após a antese e término no início da senescência dos frutos. A partir do 77º dia após a antese, os frutos foram decompostos em pericarpo, semente e arilo. A antese ocorreu na primeira semana de setembro. Foram ajustados modelos sigmoidais simples para acúmulo de matéria seca, comprimento e diâmetro. Para o acúmulo de matéria seca, uma fase inicial, de crescimento lento, prolongou-se até o 63º dia após a antese e coincidiu com um período de intensa queda natural de frutos. Do 63º ao 98º dia após a antese, houve uma fase de rápido acúmulo de matéria seca. Até o 77º dia, pericarpo e semente foram os principais responsáveis pelo acúmulo de matéria seca. Oitenta e oito por cento do comprimento e 65% do diâmetro máximos foram atingidos nesse período. Do 84º ao 98º dia após a antese, o desenvolvimento do fruto foi determinado basicamente pelo arilo. As dimensões e a matéria seca do fruto estabilizaram-se a partir do 98º dia após a antese. O ponto de colheita comercial dos frutos, caracterizado pela coloração avermelhada do pericarpo, ocorreu a partir do 112º dia após a antese. Verificou-se um quadro senescente nos frutos a partir do 119º dia após a antese.It was studied the fruit development in 'Bengal' litchi (Litchi chinensis Sonn.. The samples were taken weekly 35 days after anthesis until the beginning of fruit senescence. From the 77th day after anthesis, fruits were decomposed in pericarp, aril and seed. Anthesis occurred in the first week of September. The fruit dry matter, length and diameter followed a single-sigmoid pattern. For dry matter accumulation, an initial phase, of slow growth, ended the 63rd day after anthesis and was coincident with a period of intense fruit drop. The next phase occurred from the 63rd to the 98th day and showed a rapid dry matter

  12. Desafios enfrentados por agricultores familiares na pro