Sample records for stratified random subset

  1. On the Impact of Bootstrap in Stratified Random Sampling

    LIU Cheng; ZHAO Lian-wen


    In general the accuracy of mean estimator can be improved by stratified random sampling. In this paper, we provide an idea different from empirical methods that the accuracy can be more improved through bootstrap resampling method under some conditions. The determination of sample size by bootstrap method is also discussed, and a simulation is made to verify the accuracy of the proposed method. The simulation results show that the sample size based on bootstrapping is smaller than that based on central limit theorem.

  2. Asymptotic Properties of Random Subsets of Projective Spaces.


    large r, K takes one of at most two values dependingr on r. This theorem is analogous to a result of Bollobas and Erdos on the clique number of a random...approach does not seem eat;ily amenable to nrobabilistic analysis. The theorems of this paper may be informally summarized as follows. Fix a prime power q...Verlag, New York, Heidelberg, Berlin, 1979). 3. B. Bollobas and P. Erdo s, Cliques in random graphs, Math. Proc. Camb. Phil. Soc. 80 (1976), 419-427. 4

  3. Effects of unstratified and centre-stratified randomization in multi-centre clinical trials.

    Anisimov, Vladimir V


    This paper deals with the analysis of randomization effects in multi-centre clinical trials. The two randomization schemes most often used in clinical trials are considered: unstratified and centre-stratified block-permuted randomization. The prediction of the number of patients randomized to different treatment arms in different regions during the recruitment period accounting for the stochastic nature of the recruitment and effects of multiple centres is investigated. A new analytic approach using a Poisson-gamma patient recruitment model (patients arrive at different centres according to Poisson processes with rates sampled from a gamma distributed population) and its further extensions is proposed. Closed-form expressions for corresponding distributions of the predicted number of the patients randomized in different regions are derived. In the case of two treatments, the properties of the total imbalance in the number of patients on treatment arms caused by using centre-stratified randomization are investigated and for a large number of centres a normal approximation of imbalance is proved. The impact of imbalance on the power of the study is considered. It is shown that the loss of statistical power is practically negligible and can be compensated by a minor increase in sample size. The influence of patient dropout is also investigated. The impact of randomization on predicted drug supply overage is discussed.

  4. The large-scale placebo-controlled beta-blocker studies in systolic heart failure revisited: results from CIBIS-II, COPERNICUS and SENIORS-SHF compared with stratified subsets from MERIT-HF.

    Wikstrand, J; Wedel, H; Castagno, D; McMurray, J J V


    The four pivotal beta-blocker trials in heart failure (HF) had different inclusion criteria, making comparison difficult without patient stratifying. The aim of this study was to compare, in similar patients, the effects of bisoprolol, metoprolol controlled release/extended release (CR/XL), carvedilol and nebivolol on (i) total mortality, (ii) all-cause mortality or hospitalization due to cardiovascular causes (time to first event), (iii) all-cause mortality or hospitalization because of HF and (iv) tolerability, defined as discontinuation of randomized treatment. We compared stratified (s ) subsets in MERIT-HF with patients in CIBIS-II [New York Heart Association (NYHA) class III/IV and ejection fraction (EF) ≤ 35%] and COPERNICUS (NYHA III/IV and EF COPERNICUS (n = 2289), 19.7% vs. 12.8% (relative risk reduction 35%, 95% CI: 19-48, P = 0.0014) and MERIT-HFs (n = 795), 19.1% vs. 11.7% (relative risk reduction 39%; 95% CI: 11-58, P = 0.0086); (iii) SENIORS-SHF (n = 1359), 11.3% vs. 9.7% (relative risk reduction 16%, NS) and MERIT-HFs (n = 985), 14.8% vs. 10.1% (relative risk reduction 32%, 95% CI: 2-53, P = 0.038). The effects on the other outcomes assessed were similar. Analyses indicated fewer discontinuations from randomized treatment on beta-blockers compared with placebo in COPERNICUS and the MERIT-HFs subsets. The efficacy and tolerability of bisoprolol, carvedilol and metoprolol CR/XL are similar in patients with systolic HF, irrespective of NYHA class or ejection fraction. Nebivolol is less effective and not better tolerated. © 2013 The Association for the Publication of the Journal of Internal Medicine.

  5. Predicting protein-RNA interaction amino acids using random forest based on submodularity subset selection.

    Pan, Xiaoyong; Zhu, Lin; Fan, Yong-Xian; Yan, Junchi


    Protein-RNA interaction plays a very crucial role in many biological processes, such as protein synthesis, transcription and post-transcription of gene expression and pathogenesis of disease. Especially RNAs always function through binding to proteins. Identification of binding interface region is especially useful for cellular pathways analysis and drug design. In this study, we proposed a novel approach for binding sites identification in proteins, which not only integrates local features and global features from protein sequence directly, but also constructed a balanced training dataset using sub-sampling based on submodularity subset selection. Firstly we extracted local features and global features from protein sequence, such as evolution information and molecule weight. Secondly, the number of non-interaction sites is much more than interaction sites, which leads to a sample imbalance problem, and hence biased machine learning model with preference to non-interaction sites. To better resolve this problem, instead of previous randomly sub-sampling over-represented non-interaction sites, a novel sampling approach based on submodularity subset selection was employed, which can select more representative data subset. Finally random forest were trained on optimally selected training subsets to predict interaction sites. Our result showed that our proposed method is very promising for predicting protein-RNA interaction residues, it achieved an accuracy of 0.863, which is better than other state-of-the-art methods. Furthermore, it also indicated the extracted global features have very strong discriminate ability for identifying interaction residues from random forest feature importance analysis.

  6. Optimization of the Dutch Matrix Test by Random Selection of Sentences From a Preselected Subset

    Rolph Houben


    Full Text Available Matrix tests are available for speech recognition testing in many languages. For an accurate measurement, a steep psychometric function of the speech materials is required. For existing tests, it would be beneficial if it were possible to further optimize the available materials by increasing the function’s steepness. The objective is to show if the steepness of the psychometric function of an existing matrix test can be increased by selecting a homogeneous subset of recordings with the steepest sentence-based psychometric functions. We took data from a previous multicenter evaluation of the Dutch matrix test (45 normal-hearing listeners. Based on half of the data set, first the sentences (140 out of 311 with a similar speech reception threshold and with the steepest psychometric function (≥9.7%/dB were selected. Subsequently, the steepness of the psychometric function for this selection was calculated from the remaining (unused second half of the data set. The calculation showed that the slope increased from 10.2%/dB to 13.7%/dB. The resulting subset did not allow the construction of enough balanced test lists. Therefore, the measurement procedure was changed to randomly select the sentences during testing. Random selection may interfere with a representative occurrence of phonemes. However, in our material, the median phonemic occurrence remained close to that of the original test. This finding indicates that phonemic occurrence is not a critical factor. The work highlights the possibility that existing speech tests might be improved by selecting sentences with a steep psychometric function.

  7. Optimization of the Dutch matrix test by random selection of sentences from a preselected subset.

    Houben, Rolph; Dreschler, Wouter A


    Matrix tests are available for speech recognition testing in many languages. For an accurate measurement, a steep psychometric function of the speech materials is required. For existing tests, it would be beneficial if it were possible to further optimize the available materials by increasing the function's steepness. The objective is to show if the steepness of the psychometric function of an existing matrix test can be increased by selecting a homogeneous subset of recordings with the steepest sentence-based psychometric functions. We took data from a previous multicenter evaluation of the Dutch matrix test (45 normal-hearing listeners). Based on half of the data set, first the sentences (140 out of 311) with a similar speech reception threshold and with the steepest psychometric function (≥9.7%/dB) were selected. Subsequently, the steepness of the psychometric function for this selection was calculated from the remaining (unused) second half of the data set. The calculation showed that the slope increased from 10.2%/dB to 13.7%/dB. The resulting subset did not allow the construction of enough balanced test lists. Therefore, the measurement procedure was changed to randomly select the sentences during testing. Random selection may interfere with a representative occurrence of phonemes. However, in our material, the median phonemic occurrence remained close to that of the original test. This finding indicates that phonemic occurrence is not a critical factor. The work highlights the possibility that existing speech tests might be improved by selecting sentences with a steep psychometric function.

  8. Alcohol consumption and metabolic syndrome among Shanghai adults: A randomized multistage stratified cluster sampling investigation

    Jian-Gao Fan; Xiao-Bu Cai; Lui Li; Xing-Jian Li; Fei Dai; Jun Zhu


    AIM: To examine the relations of alcohol consumption to the prevalence of metabolic syndrome in Shanghai adults.METHODS: We performed a cross-sectional analysis of data from the randomized multistage stratified cluster sampling of Shanghai adults, who were evaluated for alcohol consumption and each component of metabolic syndrome, using the adapted U.S. National Cholesterol Education Program criteria. Current alcohol consumption was defined as more than once of alcohol drinking per month.RESULTS: The study population consisted of 3953participants (1524 men) with a mean age of 54.3 ± 12.1years. Among them, 448 subjects (11.3%) were current alcohol drinkers, including 405 males and 43 females.After adjustment for age and sex, the prevalence of current alcohol drinking and metabolic syndrome in the general population of Shanghai was 13.0% and 15.3%,respectively. Compared with nondrinkers, the prevalence of hypertriglyceridemia and hypertension was higher while the prevalence of abdominal obesity, low serum high-density-lipoprotein cholesterol (HDL-C) and diabetes mellitus was lower in subjects who consumed alcohol twice or more per month, with a trend toward reducing the prevalence of metabolic syndrome. Among the current alcohol drinkers, systolic blood pressure, HDL-C, fasting plasma glucose, and prevalence of hypertriglyceridemia tended to increase with increased alcohol consumption.However, Iow-density-lipoprotein cholesterol concentration,prevalence of abdominal obesity, low serum HDL-C andmetabolic syndrome showed the tendency to decrease.Moreover, these statistically significant differences were independent of gender and age.CONCLUSION: Current alcohol consumption is associatedwith a lower prevalence of metabolic syndrome irrespe-ctive of alcohol intake (g/d), and has a favorable influence on HDL-C, waist circumference, and possible diabetes mellitus. However, alcohol intake increases the likelihoodof hypertension, hypertriglyceridemia and hyperglycemia

  9. Nonparametric randomization-based covariate adjustment for stratified analysis of time-to-event or dichotomous outcomes.

    Hussey, Michael A; Koch, Gary G; Preisser, John S; Saville, Benjamin R


    Time-to-event or dichotomous outcomes in randomized clinical trials often have analyses using the Cox proportional hazards model or conditional logistic regression, respectively, to obtain covariate-adjusted log hazard (or odds) ratios. Nonparametric Randomization-Based Analysis of Covariance (NPANCOVA) can be applied to unadjusted log hazard (or odds) ratios estimated from a model containing treatment as the only explanatory variable. These adjusted estimates are stratified population-averaged treatment effects and only require a valid randomization to the two treatment groups and avoid key modeling assumptions (e.g., proportional hazards in the case of a Cox model) for the adjustment variables. The methodology has application in the regulatory environment where such assumptions cannot be verified a priori. Application of the methodology is illustrated through three examples on real data from two randomized trials.

  10. Second-order solutions for random interfacial waves in N-layer density stratified fluid with steady uniform currents

    Chen Xiao-Gang; Guo Zhi-Ping; Song Jin-Bao


    In the present paper,the random interfacial waves in N-layer density-stratified fluids moving at different steady uniform speeds are researched by using an expansion technique,and the second-order asymptotic solutions of the random displacements of the density interfaces and the associated velocity potentials in N-layer fluid are presented based on the small amplitude wave theory.The obtained results indicate that the wave-wave second-order nonlinear interactions of the wave components and the second-order nonlinear interactions between the waves and currents are described.As expected,the solutions include those derived by Chen(2006)as a special case where the steady uniform currents of the N-layer fluids are taken as zero,and the solutions also reduce to those obtained by Song(2005)for second-order solutions for random interracial waves with steady uniform currents if N=2.

  11. Polarimetric SAR decomposition parameter subset selection and their optimal dynamic range evaluation for urban area classification using Random Forest

    Hariharan, Siddharth; Tirodkar, Siddhesh; Bhattacharya, Avik


    Urban area classification is important for monitoring the ever increasing urbanization and studying its environmental impact. Two NASA JPL's UAVSAR datasets of L-band (wavelength: 23 cm) were used in this study for urban area classification. The two datasets used in this study are different in terms of urban area structures, building patterns, their geometric shapes and sizes. In these datasets, some urban areas appear oriented about the radar line of sight (LOS) while some areas appear non-oriented. In this study, roll invariant polarimetric SAR decomposition parameters were used to classify these urban areas. Random Forest (RF), which is an ensemble decision tree learning technique, was used in this study. RF performs parameter subset selection as a part of its classification procedure. In this study, parameter subsets were obtained and analyzed to infer scattering mechanisms useful for urban area classification. The Cloude-Pottier α, the Touzi dominant scattering amplitude αs1 and the anisotropy A were among the top six important parameters selected for both the datasets. However, it was observed that these parameters were ranked differently for the two datasets. The urban area classification using RF was compared with the Support Vector Machine (SVM) and the Maximum Likelihood Classifier (MLC) for both the datasets. RF outperforms SVM by 4% and MLC by 12% in Dataset 1. It also outperforms SVM and MLC by 3.5% and 11% respectively in Dataset 2.

  12. Evaluating effectiveness of down-sampling for stratified designs and unbalanced prevalence in Random Forest models of tree species distributions in Nevada

    Elizabeth A. Freeman; Gretchen G. Moisen; Tracy S. Frescino


    Random Forests is frequently used to model species distributions over large geographic areas. Complications arise when data used to train the models have been collected in stratified designs that involve different sampling intensity per stratum. The modeling process is further complicated if some of the target species are relatively rare on the landscape leading to an...


    T. Eisen


    Full Text Available Background. The perception that older cancer patients may be at higher risk than younger patients of toxic effects from cancer therapy but may obtain less clinical benefit from it may be based on the underrepresentation of older patients in clinical trials and the known toxic effects of cytotoxic chemotherapy. It is not known how older patients respond to targeted therapy.Methods.  This retrospective subgroup analysis of data from the phase 3, randomized Treatment Approach in Renal Cancer Global Evaluation Trial examined the safety and efficacy of sorafenib in older (age ≥ 70 years, n = 115 and younger patients (age <70 years, n = 787 who received treatment for advanced renal cell carcinoma. Patient demographics and progression-free survival were recorded. Best tumor response, clinical benefit rate (defined as complete response plus partial response plus stable disease, time to self-reported health status deterioration, and toxic effects were assessed by descriptive statistics. Health-related quality of life was assessed with a Cox proportion- al hazards model. Kaplan - Meier analyses were used to summarize time-to-event data.Results. Median progression-free survival was similar in sorafenib-treated younger patients (23.9 weeks; hazard ratio [HR] for progression compared with placebo = 0.55, 95% confidence interval [CI] = 0.47 to 0.66 and older patients (26.3 weeks; HR = 0.43, 95% CI = 0.26 to 0.69. Clinical benefit rates among younger and older sorafenib-treated patients were also similar (83.5% and 84.3%, respectively and were superior to those of younger and older placebo-treated patients (53.8% and 62.2%, respectively. Adverse events were predictable and manageable regardless of age. Sorafenib treatment delayed the time to self-reported health status deterioration among both older patients (121 days with sorafenib vs 85 days with placebo; HR = 0.66, 95% CI = 0.43 to 1.03 and younger patients (90 days with sorafenib vs 52 days with placebo


    T. Eisen


    Full Text Available Background. The perception that older cancer patients may be at higher risk than younger patients of toxic effects from cancer therapy but may obtain less clinical benefit from it may be based on the underrepresentation of older patients in clinical trials and the known toxic effects of cytotoxic chemotherapy. It is not known how older patients respond to targeted therapy.Methods.  This retrospective subgroup analysis of data from the phase 3, randomized Treatment Approach in Renal Cancer Global Evaluation Trial examined the safety and efficacy of sorafenib in older (age ≥ 70 years, n = 115 and younger patients (age <70 years, n = 787 who received treatment for advanced renal cell carcinoma. Patient demographics and progression-free survival were recorded. Best tumor response, clinical benefit rate (defined as complete response plus partial response plus stable disease, time to self-reported health status deterioration, and toxic effects were assessed by descriptive statistics. Health-related quality of life was assessed with a Cox proportion- al hazards model. Kaplan - Meier analyses were used to summarize time-to-event data.Results. Median progression-free survival was similar in sorafenib-treated younger patients (23.9 weeks; hazard ratio [HR] for progression compared with placebo = 0.55, 95% confidence interval [CI] = 0.47 to 0.66 and older patients (26.3 weeks; HR = 0.43, 95% CI = 0.26 to 0.69. Clinical benefit rates among younger and older sorafenib-treated patients were also similar (83.5% and 84.3%, respectively and were superior to those of younger and older placebo-treated patients (53.8% and 62.2%, respectively. Adverse events were predictable and manageable regardless of age. Sorafenib treatment delayed the time to self-reported health status deterioration among both older patients (121 days with sorafenib vs 85 days with placebo; HR = 0.66, 95% CI = 0.43 to 1.03 and younger patients (90 days with sorafenib vs 52 days with placebo

  15. Development and Assessment of an E-Learning Course on Breast Imaging for Radiographers: A Stratified Randomized Controlled Trial

    Ventura, Sandra Rua; Ramos, Isabel; Rodrigues, Pedro Pereira


    Background Mammography is considered the best imaging technique for breast cancer screening, and the radiographer plays an important role in its performance. Therefore, continuing education is critical to improving the performance of these professionals and thus providing better health care services. Objective Our goal was to develop an e-learning course on breast imaging for radiographers, assessing its efficacy, effectiveness, and user satisfaction. Methods A stratified randomized controlled trial was performed with radiographers and radiology students who already had mammography training, using pre- and post-knowledge tests, and satisfaction questionnaires. The primary outcome was the improvement in test results (percentage of correct answers), using intention-to-treat and per-protocol analysis. Results A total of 54 participants were assigned to the intervention (20 students plus 34 radiographers) with 53 controls (19+34). The intervention was completed by 40 participants (11+29), with 4 (2+2) discontinued interventions, and 10 (7+3) lost to follow-up. Differences in the primary outcome were found between intervention and control: 21 versus 4 percentage points (pp), Pstudents (18 pp vs 5 pp; P=.098). Nonetheless, differences in students’ posttest results were found (88% vs 63%; P=.003), which were absent in pretest (63% vs 63%; P=.106). The per-protocol analysis showed a higher effect (26 pp vs 2 pp; Pstudents (25 pp vs 3 pp; P=.004) and radiographers (27 pp vs 2 pp; P<.001). Overall, 85% were satisfied with the course, and 88% considered it successful. Conclusions This e-learning course is effective, especially for radiographers, which highlights the need for continuing education. PMID:25560547

  16. Optimal Subset Selection of Time-Series MODIS Images and Sample Data Transfer with Random Forests for Supervised Classification Modelling.

    Zhou, Fuqun; Zhang, Aining


    Nowadays, various time-series Earth Observation data with multiple bands are freely available, such as Moderate Resolution Imaging Spectroradiometer (MODIS) datasets including 8-day composites from NASA, and 10-day composites from the Canada Centre for Remote Sensing (CCRS). It is challenging to efficiently use these time-series MODIS datasets for long-term environmental monitoring due to their vast volume and information redundancy. This challenge will be greater when Sentinel 2-3 data become available. Another challenge that researchers face is the lack of in-situ data for supervised modelling, especially for time-series data analysis. In this study, we attempt to tackle the two important issues with a case study of land cover mapping using CCRS 10-day MODIS composites with the help of Random Forests' features: variable importance, outlier identification. The variable importance feature is used to analyze and select optimal subsets of time-series MODIS imagery for efficient land cover mapping, and the outlier identification feature is utilized for transferring sample data available from one year to an adjacent year for supervised classification modelling. The results of the case study of agricultural land cover classification at a regional scale show that using only about a half of the variables we can achieve land cover classification accuracy close to that generated using the full dataset. The proposed simple but effective solution of sample transferring could make supervised modelling possible for applications lacking sample data.

  17. A stratified random survey of the proportion of poor quality oral artesunate sold at medicine outlets in the Lao PDR – implications for therapeutic failure and drug resistance

    Vongsack Latsamy


    Full Text Available Abstract Background Counterfeit oral artesunate has been a major public health problem in mainland SE Asia, impeding malaria control. A countrywide stratified random survey was performed to determine the availability and quality of oral artesunate in pharmacies and outlets (shops selling medicines in the Lao PDR (Laos. Methods In 2003, 'mystery' shoppers were asked to buy artesunate tablets from 180 outlets in 12 of the 18 Lao provinces. Outlets were selected using stratified random sampling by investigators not involved in sampling. Samples were analysed for packaging characteristics, by the Fast Red Dye test, high-performance liquid chromatography (HPLC, mass spectrometry (MS, X-ray diffractometry and pollen analysis. Results Of 180 outlets sampled, 25 (13.9% sold oral artesunate. Outlets selling artesunate were more commonly found in the more malarious southern Laos. Of the 25 outlets, 22 (88%; 95%CI 68–97% sold counterfeit artesunate, as defined by packaging and chemistry. No artesunate was detected in the counterfeits by any of the chemical analysis techniques and analysis of the packaging demonstrated seven different counterfeit types. There was complete agreement between the Fast Red dye test, HPLC and MS analysis. A wide variety of wrong active ingredients were found by MS. Of great concern, 4/27 (14.8% fakes contained detectable amounts of artemisinin (0.26–115.7 mg/tablet. Conclusion This random survey confirms results from previous convenience surveys that counterfeit artesunate is a severe public health problem. The presence of artemisinin in counterfeits may encourage malaria resistance to artemisinin derivatives. With increasing accessibility of artemisinin-derivative combination therapy (ACT in Laos, the removal of artesunate monotherapy from pharmacies may be an effective intervention.

  18. Evaluating results from the multiple myeloma patient subset treated with denosumab or zoledronic acid in a randomized phase 3 trial.

    Raje, N; Vadhan-Raj, S; Willenbacher, W; Terpos, E; Hungria, V; Spencer, A; Alexeeva, Y; Facon, T; Stewart, A K; Feng, A; Braun, A; Balakumaran, A; Roodman, G D


    In a phase 3 trial of denosumab vs zoledronic acid in patients (n=1776) with bone metastases and solid tumors or multiple myeloma, denosumab was superior to zoledronic acid for the primary end point of prevention of skeletal-related events. There was no difference in overall survival between the two groups; however, an ad hoc overall survival analysis in the multiple myeloma subset of patients (n=180) favored zoledronic acid (hazard ratio (HR) 2.26; 95% confidence interval (CI) 1.13-4.50; P=0.014). In the present analysis, we found imbalances between the groups with respect to baseline risk characteristics. HRs with two-sided 95% CIs were estimated using the Cox model. After adjustment in a covariate analysis, the CI crossed unity (HR 1.86; 95% CI 0.90-3.84; P=0.0954). Furthermore, we found a higher rate of early withdrawals for the reasons of lost to follow-up and withdrawal of consent in the zoledronic acid group; after accounting for these, the HR was 1.31 (95% CI 0.80-2.15; P=0.278). In conclusion, the survival results in multiple myeloma patients in this trial were confounded and will eventually be resolved by an ongoing phase 3 trial.

  19. Mesohaline submerged aquatic vegetation survey along the U.S. gulf of Mexico coast, 2000: A stratified random approach

    Carter, J.; Merino, J.H.; Merino, S.L.


    Estimates of submerged aquatic vegetative (SAV) along the U.S. Gulf of Mexico (Gulf) generally focus on seagrasses. In 2000, we attempted a synoptic survey of SAV in the mesohaline (5-20 ppt) zone of estuarine and nearshore areas of the northeastern Gulf. Areas with SAV were identified from existing aerial 1992 photography, and a literature review was used to select those areas that were likely to experience mesohaline conditions during the growing season. In 2000, a drought year, we visited 217 randomly selected SAV beds and collected data on species composition and environmental conditions. In general, sites were either clearly polyhaline (2: 20 ppt) or oligohaline (S 5 ppt), with only five sites measuring between 5 and 20 ppt. Ruppia maritima L. (13-35 ppt, n = 28) was the only species that occurred in mesohaline salinities. Halodule wrightii Asch. occurred in 73% of the beds. The nonindigenous Myriophyllum spicatum L. was present in four locations with salinities below 3 ppt. No nonindigenous macroalgae were identified, and no nonindigenous angiosperms occurred in salinities above 3 ppt. Selecting sample locations based on historical salinity data was not a successful strategy for surveying SAV in mesohaline systems, particularly during a drought year. Our ability to locate SAV beds within 50 m of their aerially located position 8 yr later demonstrates some SAV stability in the highly variable conditions of the study area. ?? 2009 by the Marine Environmental Silences Consortium of Alabama.

  20. A profile of US-Mexico border mobility among a stratified random sample of Hispanics living in the El Paso-Juarez area.

    Lapeyrouse, L M; Morera, O; Heyman, J M C; Amaya, M A; Pingitore, N E; Balcazar, H


    Examination of border-specific characteristics such as trans-border mobility and transborder health service illuminates the heterogeneity of border Hispanics and may provide greater insight toward understanding differential health behaviors and status among these populations. In this study, we create a descriptive profile of the concept of trans-border mobility by exploring the relationship between mobility status and a series of demographic, economic and socio-cultural characteristics among mobile and non-mobile Hispanics living in the El Paso-Juarez border region. Using a two-stage stratified random sampling design, bilingual interviewers collected survey data from border residents (n = 1,002). Findings show that significant economic, cultural, and behavioral differences exist between mobile and non-mobile respondents. While non-mobile respondents were found to have higher social economic status than their mobile counterparts, mobility across the border was found to offer less acculturated and poorer Hispanics access to alternative sources of health care and other services.

  1. A description of the demographic characteristics of the New Zealand non-commercial horse population with data collected using a generalised random-tessellation stratified sampling design.

    Rosanowski, S M; Cogger, N; Rogers, C W; Benschop, J; Stevenson, M A


    We conducted a cross-sectional survey to determine the demographic characteristics of non-commercial horses in New Zealand. A sampling frame of properties with non-commercial horses was derived from the national farms database, AgriBase™. Horse properties were stratified by property size and a generalised random-tessellated stratified (GRTS) sampling strategy was used to select properties (n=2912) to take part in the survey. The GRTS sampling design allowed for the selection of properties that were spatially balanced relative to the distribution of horse properties throughout the country. The registered decision maker of the property, as identified in AgriBase™, was sent a questionnaire asking them to describe the demographic characteristics of horses on the property, including the number and reason for keeping horses, as well as information about other animals kept on the property and the proximity of boundary neighbours with horses. The response rate to the survey was 38% (1044/2912) and the response rate was not associated with property size or region. A total of 5322 horses were kept for recreation, competition, racing, breeding, stock work, or as pets. The reasons for keeping horses and the number and class of horses varied significantly between regions and by property size. Of the properties sampled, less than half kept horses that could have been registered with Equestrian Sports New Zealand or either of the racing codes. Of the respondents that reported knowing whether their neighbours had horses, 58.6% (455/776) of properties had at least one boundary neighbour that kept horses. The results of this study have important implications for New Zealand, which has an equine population that is naïve to many equine diseases considered endemic worldwide. The ability to identify, and apply accurate knowledge of the population at risk to infectious disease control strategies would lead to more effective strategies to control and prevent disease spread during an

  2. Gambling Participation and Problem Gambling Severity in a Stratified Random Survey: Findings from the Second Social and Economic Impact Study of Gambling in Tasmania.

    Christensen, Darren R; Dowling, Nicki A; Jackson, Alun C; Thomas, Shane A


    Demographic characteristics associated with gambling participation and problem gambling severity were investigated in a stratified random survey in Tasmania, Australia. Computer-assisted telephone interviews were conducted in March 2011 resulting in a representative sample of 4,303 Tasmanian residents aged 18 years or older. Overall, 64.8% of Tasmanian adults reported participating in some form of gambling in the previous 12 months. The most common forms of gambling were lotteries (46.5%), keno (24.3%), instant scratch tickets (24.3%), and electronic gaming machines (20.5%). Gambling severity rates were estimated at non-gambling (34.8%), non-problem gambling (57.4%), low risk gambling (5.3%), moderate risk (1.8%), and problem gambling (.7%). Compared to Tasmanian gamblers as a whole significantly higher annual participation rates were reported by couples with no children, those in full time paid employment, and people who did not complete secondary school. Compared to Tasmanian gamblers as a whole significantly higher gambling frequencies were reported by males, people aged 65 or older, and people who were on pensions or were unable to work. Compared to Tasmanian gamblers as a whole significantly higher gambling expenditure was reported by males. The highest average expenditure was for horse and greyhound racing ($AUD 1,556), double the next highest gambling activity electronic gaming machines ($AUD 767). Compared to Tasmanian gamblers as a whole problem gamblers were significantly younger, in paid employment, reported lower incomes, and were born in Australia. Although gambling participation rates appear to be falling, problem gambling severity rates remain stable. These changes appear to reflect a maturing gambling market and the need for population specific harm minimisation strategies.

  3. Subset Analysis of a Multicenter, Randomized Controlled Trial to Compare Magnifying Chromoendoscopy with Endoscopic Ultrasonography for Stage Diagnosis of Early Stage Colorectal Cancer.

    Tomonori Yamada

    Full Text Available Our recent prospective study found equivalent accuracy of magnifying chromoendoscopy (MC and endoscopic ultrasonography (EUS for diagnosing the invasion depth of colorectal cancer (CRC; however, whether these tools show diagnostic differences in categories such as tumor size and morphology remains unclear. Hence, we conducted detailed subset analysis of the prospective data.In this multicenter, prospective, comparative trial, a total of 70 patients with early, flat CRC were enrolled from February 2011 to December 2012, and the results of 66 lesions were finally analyzed. Patients were randomly allocated to primary MC followed by EUS or to primary EUS followed by MC. Diagnoses of invasion depth by each tool were divided into intramucosal to slight submucosal invasion (invasion depth <1000 μm and deep submucosal invasion (invasion depth ≥1000 μm, and then compared with the final pathological diagnosis by an independent pathologist blinded to clinical data. To standardize diagnoses among examiners, this trial was started after achievement of a mean κ value of ≥0.6 which was calculated from the average of κ values between each pair of participating endoscopists.Both MC and EUS showed similar diagnostic outcomes, with no significant differences in prediction of invasion depth in subset analyses according to tumor size, location, and morphology. Lesions that were consistently diagnosed as Tis/T1-SMS or ≥T1-SMD with both tools revealed accuracy of 76-78%. Accuracy was low in borderline lesions with irregular pit pattern in MC and distorted findings of the third layer in EUS (MC, 58.5%; EUS, 50.0%.MC and EUS showed the same limited accuracy for predicting invasion depth in all categories of early CRC. Since the irregular pit pattern in MC, distorted findings to the third layer in EUS and inconsistent diagnosis between both tools were associated with low accuracy, further refinements or even novel methods are still needed for such lesions

  4. Subsets of configurations and canonical partition functions

    Bloch, J.; Bruckmann, F.; Kieburg, M.;


    We explain the physical nature of the subset solution to the sign problem in chiral random matrix theory: the subset sum over configurations is shown to project out the canonical determinant with zero quark charge from a given configuration. As the grand canonical chiral random matrix partition...

  5. Mining Representative Subset Based on Fuzzy Clustering

    ZHOU Hongfang; FENG Boqin; L(U) Lintao


    Two new concepts-fuzzy mutuality and average fuzzy entropy are presented. Then based on these concepts, a new algorithm-RSMA (representative subset mining algorithm) is proposed, which can abstract representative subset from massive data.To accelerate the speed of producing representative subset, an improved algorithm-ARSMA(accelerated representative subset mining algorithm) is advanced, which adopt combining putting forward with backward strategies. In this way, the performance of the algorithm is improved. Finally we make experiments on real datasets and evaluate the representative subset. The experiment shows that ARSMA algorithm is more excellent than RandomPick algorithm either on effectiveness or efficiency.

  6. Design and methodology of the NorthStar Study: NT-proBNP stratified follow-up in outpatient heart failure clinics -- a randomized Danish multicenter study

    Schou, Morten; Gustafsson, Finn; Videbaek, Lars;


    BACKGROUND: Randomized clinical trials have shown that newly discharged and symptomatic patients with chronic heart failure (CHF) benefit from follow-up in a specialized heart failure clinic (HFC). Clinical stable and educated patients are usually discharged from the HFC when on optimal therapy....... It is unknown if risk stratification using natriuretic peptides could identify patients who would benefit from longer-term follow-up. Furthermore, data on the use of natriuretic peptides for monitoring of stable patients with CHF are sparse. AIMS: The aims of this study are to test the hypothesis that clinical...... stable, educated, and medical optimized patients with CHF with N-terminal pro-brain natriuretic peptide (NT-proBNP) levels > or = 1,000 pg/mL benefit from long-term follow-up in an HFC and to assess the efficacy of NT-proBNP monitoring. METHODS: A total of 1,250 clinically stable, medically optimized...

  7. Operative strategies to reduce cerebral embolic events during on- and off-pump coronary artery bypass surgery: A stratified, prospective randomized trial.

    Halkos, Michael E; Anderson, Aaron; Binongo, Jose Nilo G; Stringer, Anthony; Lasanajak, Yi; Thourani, Vinod H; Lattouf, Omar M; Guyton, Robert A; Baio, Kim T; Sarin, Eric; Keeling, William B; Cook, N Renee; Carssow, Katherine; Neill, Alexis; Glas, Kathryn E; Puskas, John D


    To determine the impact of different aortic clamping strategies on the incidence of cerebral embolic events during coronary artery bypass grafting (CABG). Between 2012 and 2015, 142 patients with low-grade aortic disease (epiaortic ultrasound grade I/II) undergoing primary isolated CABG were studied. Those undergoing off-pump CABG were randomized to a partial clamp (n = 36) or clampless facilitating device (CFD; n = 36) strategy. Those undergoing on-pump CABG were randomized to a single-clamp (n = 34) or double-clamp (n = 36) strategy. Transcranial Doppler ultrasonography (TCD) was performed to identify high-intensity transient signals (HITS) in the middle cerebral arteries during periods of aortic manipulation. Neurocognitive testing was performed at baseline and 30-days postoperatively. The primary endpoint was total number of HITS detected by TCD. Groups were compared using the Mann-Whitney U test. In the off-pump group, the median number of total HITS were higher in the CFD subgroup (30.0; interquartile range [IQR], 22-43) compared with the partial clamp subgroup (7.0; IQR, 0-16; P 1 CFD (12.5 [IQR, 4-19] vs 36.0 [IQR, 25-47]; P = .001). In the on-pump group, the median number of total HITS was 10.0 (IQR, 3-17) in the single-clamp group, compared with 16.0 (IQR, 4-49) in the double-clamp group (P = .10). There were no differences in neurocognitive outcomes across the groups. For patients with low-grade aortic disease, the use of CFDs was associated with an increased rate of cerebral embolic events compared with partial clamping during off-pump CABG. A single-clamp strategy during on-pump CABG did not significantly reduce embolic events compared with a double-clamp strategy. Copyright © 2017 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.

  8. Effectiveness of Early Preventive Intervention with Semiannual Fluoride Varnish Application in Toddlers Living in High-Risk Areas: A Stratified Cluster-Randomized Controlled Trial.

    Anderson, Maria; Dahllöf, Göran; Twetman, Svante; Jansson, Leif; Bergenlid, Ann-Cathrine; Grindefjord, Margaret


    This study evaluated whether toddlers in an extended preventive program of semiannual fluoride varnish applications from 1 year of age had a lower incidence of caries than those undergoing a standard program. A cohort of 1-year-old children (n = 3,403) living in multicultural areas of low socioeconomic standing in Stockholm participated in a cluster-randomized controlled field trial with two parallel arms. The children attended 23 dental clinics. Using the ICDAS II criteria, the examiners recorded caries at baseline and after 1 and 2 years. The children in the reference group received a standardized oral health program once yearly between 1 and 3 years of age. The children in the test group received the same standard program supplemented with topical applications of fluoride varnish every 6 months. We compared the test group and the reference group for the prevalence and increment of caries. At baseline, 5% of the children had already developed caries (ICDAS II 1-6). We reexamined the children after 1 year (n = 2,675) and after 2 years (n = 2,536). Neither prevalence nor caries increment differed between the groups. At 3 years of age, 12% of the children had developed moderate and severe carious lesions (ICDAS II 3-6), with a mean increment of 0.5 (SD 2.4) in the test group and 0.6 (SD 2.2) in the reference group. In conclusion, semiannual professional applications of fluoride varnish, as a supplement to a standard oral health program, failed to reduce caries development in toddlers from high-risk communities. © 2016 S. Karger AG, Basel.

  9. Unsupervised Feature Subset Selection

    Søndberg-Madsen, Nicolaj; Thomsen, C.; Pena, Jose


    This paper studies filter and hybrid filter-wrapper feature subset selection for unsupervised learning (data clustering). We constrain the search for the best feature subset by scoring the dependence of every feature on the rest of the features, conjecturing that these scores discriminate some...... irrelevant features. We report experimental results on artificial and real data for unsupervised learning of naive Bayes models. Both the filter and hybrid approaches perform satisfactorily....

  10. Subset selection in regression

    Miller, Alan


    Originally published in 1990, the first edition of Subset Selection in Regression filled a significant gap in the literature, and its critical and popular success has continued for more than a decade. Thoroughly revised to reflect progress in theory, methods, and computing power, the second edition promises to continue that tradition. The author has thoroughly updated each chapter, incorporated new material on recent developments, and included more examples and references. New in the Second Edition:A separate chapter on Bayesian methodsComplete revision of the chapter on estimationA major example from the field of near infrared spectroscopyMore emphasis on cross-validationGreater focus on bootstrappingStochastic algorithms for finding good subsets from large numbers of predictors when an exhaustive search is not feasible Software available on the Internet for implementing many of the algorithms presentedMore examplesSubset Selection in Regression, Second Edition remains dedicated to the techniques for fitting...

  11. Fluttering in Stratified Flows

    Lam, Try; Vincent, Lionel; Kanso, Eva


    The descent motion of heavy objects under the influence of gravitational and aerodynamic forces is relevant to many branches of engineering and science. Examples range from estimating the behavior of re-entry space vehicles to studying the settlement of marine larvae and its influence on underwater ecology. The behavior of regularly shaped objects freely falling in homogeneous fluids is relatively well understood. For example, the complex interaction of a rigid coin with the surrounding fluid will cause it to either fall steadily, flutter, tumble, or be chaotic. Less is known about the effect of density stratification on the descent behavior. Here, we experimentally investigate the descent of discs in both pure water and in a linearly salt-stratified fluids where the density is varied from 1.0 to 1.14 of that of water where the Brunt-Vaisala frequency is 1.7 rad/sec and the Froude number Fr robots for space exploration and underwater missions.

  12. Epistemically Robust Strategy Subsets

    Geir B. Asheim


    Full Text Available We define a concept of epistemic robustness in the context of an epistemic model of a finite normal-form game where a player type corresponds to a belief over the profiles of opponent strategies and types. A Cartesian product X of pure-strategy subsets is epistemically robust if there is a Cartesian product Y of player type subsets with X as the associated set of best reply profiles such that the set Y i contains all player types that believe with sufficient probability that the others are of types in Y − i and play best replies. This robustness concept provides epistemic foundations for set-valued generalizations of strict Nash equilibrium, applicable also to games without strict Nash equilibria. We relate our concept to closedness under rational behavior and thus to strategic stability and to the best reply property and thus to rationalizability.

  13. Prediction based on mean subset

    Øjelund, Henrik; Brown, P. J.; Madsen, Henrik


    Shrinkage methods have traditionally been applied in prediction problems. In this article we develop a shrinkage method (mean subset) that forms an average of regression coefficients from individual subsets of the explanatory variables. A Bayesian approach is taken to derive an expression of how...... the coefficient vectors from each subset should be weighted. It is not computationally feasible to calculate the mean subset coefficient vector for larger problems, and thus we suggest an algorithm to find an approximation to the mean subset coefficient vector. In a comprehensive Monte Carlo simulation study...

  14. Prediction based on mean subset

    Øjelund, Henrik; Brown, P. J.; Madsen, Henrik;


    , it is found that the proposed mean subset method has superior prediction performance than prediction based on the best subset method, and in some settings also better than the ridge regression and lasso methods. The conclusions drawn from the Monte Carlo study is corroborated in an example in which prediction......Shrinkage methods have traditionally been applied in prediction problems. In this article we develop a shrinkage method (mean subset) that forms an average of regression coefficients from individual subsets of the explanatory variables. A Bayesian approach is taken to derive an expression of how...

  15. Electromagnetic waves in stratified media

    Wait, James R; Fock, V A; Wait, J R


    International Series of Monographs in Electromagnetic Waves, Volume 3: Electromagnetic Waves in Stratified Media provides information pertinent to the electromagnetic waves in media whose properties differ in one particular direction. This book discusses the important feature of the waves that enables communications at global distances. Organized into 13 chapters, this volume begins with an overview of the general analysis for the electromagnetic response of a plane stratified medium comprising of any number of parallel homogeneous layers. This text then explains the reflection of electromagne

  16. Stratified medicine and reimbursement issues

    Fugel, Hans-Joerg; Nuijten, Mark; Postma, Maarten


    Stratified Medicine (SM) has the potential to target patient populations who will most benefit from a therapy while reducing unnecessary health interventions associated with side effects. The link between clinical biomarkers/diagnostics and therapies provides new opportunities for value creation to

  17. Stratified Medicine and Reimbursement Issues

    Hans-Joerg eFugel


    Full Text Available Stratified Medicine (SM has the potential to target patient populations who will most benefit from a therapy while reducing unnecessary health interventions associated with side effects. The link between clinical biomarkers/diagnostics and therapies provides new opportunities for value creation to strengthen the value proposition to pricing and reimbursement (P&R authorities. However, the introduction of SM challenges current reimbursement schemes in many EU countries and the US as different P&R policies have been adopted for drugs and diagnostics. Also, there is a lack of a consistent process for value assessment of more complex diagnostics in these markets. New, innovative approaches and more flexible P&R systems are needed to reflect the added value of diagnostic tests and to stimulate investments in new technologies. Yet, the framework for access of diagnostic–based therapies still requires further development while setting the right incentives and appropriate align stakeholders interests when realizing long- term patient benefits. This article addresses the reimbursement challenges of SM approaches in several EU countries and the US outlining some options to overcome existing reimbursement barriers for stratified medicine.

  18. Suppression of stratified explosive interactions

    Meeks, M.K.; Shamoun, B.I.; Bonazza, R.; Corradini, M.L. [Wisconsin Univ., Madison, WI (United States). Dept. of Nuclear Engineering and Engineering Physics


    Stratified Fuel-Coolant Interaction (FCI) experiments with Refrigerant-134a and water were performed in a large-scale system. Air was uniformly injected into the coolant pool to establish a pre-existing void which could suppress the explosion. Two competing effects due to the variation of the air flow rate seem to influence the intensity of the explosion in this geometrical configuration. At low flow rates, although the injected air increases the void fraction, the concurrent agitation and mixing increases the intensity of the interaction. At higher flow rates, the increase in void fraction tends to attenuate the propagated pressure wave generated by the explosion. Experimental results show a complete suppression of the vapor explosion at high rates of air injection, corresponding to an average void fraction of larger than 30%. (author)

  19. B cell subsets in atherosclerosis

    Heather M. Perry


    Full Text Available Atherosclerosis, the underlying cause of heart attacks and strokes, is a chronic inflammatory disease of the artery wall. Immune cells, including lymphocytes modulate atherosclerotic lesion development through interconnected mechanisms. Elegant studies over the past decades have begun to unravel a role for B cells in atherosclerosis. Recent findings provide evidence that B cell effects on atherosclerosis may be subset-dependent. B-1a B cells have been reported to protect from atherosclerosis by secretion of natural IgM antibodies. Conventional B-2 B cells can promote atherosclerosis through less clearly defined mechanism that may involve CD4 T cells. Yet, there may be other populations of B cells within these subsets with different phenotypes altering their impact on atherosclerosis. Additionally, the role of B cell subsets in atherosclerosis may depend on their environmental niche and/or the stage of atherogenesis. This review will highlight key findings in the evolving field of B cells and atherosclerosis and touch on the potential and importance of translating these findings to human disease.

  20. Helicity dynamics in stratified turbulence in the absence of forcing

    Rorai, C; Pouquet, A; Mininni, P D


    A numerical study of decaying stably-stratified flows is performed. Relatively high stratification and moderate Reynolds numbers are considered, and a particular emphasis is placed on the role of helicity (velocity-vorticity correlations). The problem is tackled by integrating the Boussinesq equations in a periodic cubical domain using different initial conditions: a non-helical Taylor-Green (TG) flow, a fully helical Beltrami (ABC) flow, and random flows with a tunable helicity. We show that for stratified ABC flows helicity undergoes a substantially slower decay than for unstratified ABC flows. This fact is likely associated to the combined effect of stratification and large scale coherent structures. Indeed, when the latter are missing, as in random flows, helicity is rapidly destroyed by the onset of gravitational waves. A type of large-scale dissipative "cyclostrophic" balance can be invoked to explain this behavior. When helicity survives in the system it strongly affects the temporal energy decay and t...

  1. Stratified wake of an accelerating hydrofoil

    Ben-Gida, Hadar; Gurka, Roi


    Wakes of towed and self-propelled bodies in stratified fluids are significantly different from non-stratified wakes. Long time effects of stratification on the development of the wakes of bluff bodies moving at constant speed are well known. In this experimental study we demonstrate how buoyancy affects the initial growth of vortices developing in the wake of a hydrofoil accelerating from rest. Particle image velocimetry measurements were applied to characterize the wake evolution behind a NACA 0015 hydrofoil accelerating in water and for low Reynolds number and relatively strong and stably stratified fluid (Re=5,000, Fr~O(1)). The analysis of velocity and vorticity fields, following vortex identification and an estimate of the circulation, reveal that the vortices in the stratified fluid case are stretched along the streamwise direction in the near wake. The momentum thickness profiles show lower momentum thickness values for the stratified late wake compared to the non-stratified wake, implying that the dra...

  2. How stratified is mantle convection?

    Puster, Peter; Jordan, Thomas H.


    We quantify the flow stratification in the Earth's mid-mantle (600-1500 km) in terms of a stratification index for the vertical mass flux, Sƒ (z) = 1 - ƒ(z) / ƒref (z), in which the reference value ƒref(z) approximates the local flux at depth z expected for unstratified convection (Sƒ=0). Although this flux stratification index cannot be directly constrained by observations, we show from a series of two-dimensional convection simulations that its value can be related to a thermal stratification index ST(Z) defined in terms of the radial correlation length of the temperature-perturbation field δT(z, Ω). ST is a good proxy for Sƒ at low stratifications (SƒUniformitarian Principle. The bound obtained here from global tomography is consistent with local seismological evidence for slab flux into the lower mantle; however, the total material flux has to be significantly greater (by a factor of 2-3) than that due to slabs alone. A stratification index, Sƒ≲0.2, is sufficient to exclude many stratified convection models still under active consideration, including most forms of chemical layering between the upper and lower mantle, as well as the more extreme versions of avalanching convection governed by a strong endothermic phase change.


    Liu Yongguo; Li Xueming; Wu Zhongfu


    The motivation of data mining is how to extract effective information from huge data in very large database. However, some redundant and irrelevant attributes, which result in low performance and high computing complexity, are included in the very large database in general.So, Feature Subset Selection (FSS) becomes one important issue in the field of data mining. In this letter, an FSS model based on the filter approach is built, which uses the simulated annealing genetic algorithm. Experimental results show that convergence and stability of this algorithm are adequately achieved.

  4. Core science: Stratified by a sunken impactor

    Nakajima, Miki


    There is potential evidence for a stratified layer at the top of the Earth's core, but its origin is not well understood. Laboratory experiments suggest that the stratified layer could be a sunken remnant of the giant impact that formed the Moon.

  5. A Fixpoint Semantics for Stratified Databases



    Przmusinski extended the notion of stratified logic programs,developed by Apt,Blair and Walker,and by van Gelder,to stratified databases that allow both negative premises and disjunctive consequents.However,he did not provide a fixpoint theory for such class of databases.On the other hand,although a fixpoint semantics has been developed by Minker and Rajasekar for non-Horn logic programs,it is tantamount to traditional minimal model semantics which is not sufficient to capture the intended meaning of negation in the premises of clauses in stratified databases.In this paper,a fixpoint approach to stratified databases is developed,which corresponds with the perfect model semantics.Moreover,algorithms are proposed for computing the set of perfect models of a stratified database.

  6. Randomized phase III trial of APF530 versus palonosetron in the prevention of chemotherapy-induced nausea and vomiting in a subset of patients with breast cancer receiving moderately or highly emetogenic chemotherapy.

    Boccia, Ralph; O'Boyle, Erin; Cooper, William


    APF530 provides controlled, sustained-release granisetron for preventing acute (0-24 h) and delayed (24-120 h) chemotherapy-induced nausea and vomiting (CINV). In a phase III trial, APF530 was noninferior to palonosetron in preventing acute CINV following single-dose moderately (MEC) or highly emetogenic chemotherapy (HEC) and delayed CINV in MEC (MEC and HEC defined by Hesketh criteria). This exploratory subanalysis was conducted in the breast cancer subpopulation. Patients were randomized to subcutaneous APF530 250 or 500 mg (granisetron 5 or 10 mg) or intravenous palonosetron 0.25 mg during cycle 1. Palonosetron patients were randomized to APF530 for cycles 2 to 4. The primary efficacy end point was complete response (CR, no emesis or rescue medication) in cycle 1. Among breast cancer patients (n = 423 MEC, n = 185 HEC), > 70 % received anthracycline-containing regimens in each emetogenicity subgroup. There were no significant between-group differences in CRs in cycle 1 for acute (APF530 250 mg: MEC 71 %, HEC 77 %; 500 mg: MEC 73 %, HEC 73 %; palonosetron: MEC 68 %, HEC 66 %) and delayed (APF530 250 mg: MEC 46 %, HEC 58 %; 500 mg: MEC 48 %, HEC 63 %; palonosetron: MEC 52 %, HEC 52 %) CINV. There were no significant differences in within-cycle CRs between APF530 doses for acute and delayed CINV in MEC or HEC in cycles 2 to 4; CRs trended higher in later cycles, with no notable differences in adverse events between breast cancer and overall populations. APF530 effectively prevented acute and delayed CINV over 4 chemotherapy cycles in breast cancer patients receiving MEC or HEC. identifier: NCT00343460 (June 22, 2006).

  7. Addition of cladribine to the standard induction treatment improves outcomes in a subset of elderly acute myeloid leukemia patients. Results of a randomized Polish Adult Leukemia Group (PALG) phase II trial.

    Pluta, Agnieszka; Robak, Tadeusz; Wrzesien-Kus, Agata; Katarzyna Budziszewska, Bozena; Sulek, Kazimierz; Wawrzyniak, Ewa; Czemerska, Magdalena; Zwolinska, Malgorzata; Golos, Aleksandra; Holowiecka-Goral, Aleksandra; Kyrcz-Krzemien, Slawomira; Piszcz, Jaroslaw; Kloczko, Janusz; Mordak-Domagala, Monika; Lange, Andrzej; Razny, Małgorzata; Madry, Krzysztof; Wiktor-Jedrzejczak, Wieslaw; Grosicki, Sebastian; Butrym, Aleksandra; Kuliczkowski, Kazimierz; Warzocha, Krzysztof; Holowiecki, Jerzy; Giebel, Sebastian; Szydlo, Richard; Wierzbowska, Agnieszka


    Intensive induction chemotherapy using anthracycline and cytarabine backbone is considered the most effective upfront therapy in physically fit older patients with acute myeloid leukemia (AML). However, outcomes of the standard induction in elderly AML are inferior to those observed in younger patients, and they are still unsatisfactory. As addition of cladribine to the standard induction therapy is known to improve outcome in younger AML patients. The present randomized phase II study compares efficacy and toxicity of the DAC (daunorubicin plus cytarabine plus cladribine) regimen with the standard DA (daunorubicin plus cytarabine) regimen in the newly diagnosed AML patients over 60 years of age. A total of 171 patients were enrolled in the study (DA, 86; DAC, 85). A trend toward higher complete remission (CR) was observed in the DAC arm compared to the DA arm (44% vs. 34%; P = .19), which did not lead to improved median overall survival, which in the case of the DAC group was 8.6 months compared to in 9.1 months in the DA group (P = .64). However, DAC appeared to be superior in the group of patients aged 60-65 (CR rate: DAC 51% vs. DA 29%; P = .02). What is more, a subgroup of patients, with good and intermediate karyotypes, benefited from addition of cladribine also in terms of overall survival (P = .02). No differences in hematological and nonhematological toxicity between the DA and DAC regimens were observed.

  8. MRE11-deficiency associated with improved long-term disease free survival and overall survival in a subset of stage III colon cancer patients in randomized CALGB 89803 trial.

    Thomas Pavelitz

    Full Text Available PURPOSE: Colon cancers deficient in mismatch repair (MMR may exhibit diminished expression of the DNA repair gene, MRE11, as a consequence of contraction of a T11 mononucleotide tract. This study investigated MRE11 status and its association with prognosis, survival and drug response in patients with stage III colon cancer. PATIENTS AND METHODS: Cancer and Leukemia Group B 89803 (Alliance randomly assigned 1,264 patients with stage III colon cancer to postoperative weekly adjuvant bolus 5-fluorouracil/leucovorin (FU/LV or irinotecan+FU/LV (IFL, with 8 year follow-up. Tumors from these patients were analyzed to determine stability of a T11 tract in the MRE11 gene. The primary endpoint was overall survival (OS, and a secondary endpoint was disease-free survival (DFS. Non-proportional hazards were addressed using time-dependent covariates in Cox analyses. RESULTS: Of 625 tumor cases examined, 70 (11.2% exhibited contraction at the T11 tract in one or both MRE11 alleles and were thus predicted to be deficient in MRE11 (dMRE11. In pooled treatment analyses, dMRE11 patients showed initially reduced DFS and OS but improved long-term DFS and OS compared with patients with an intact MRE11 T11 tract. In the subgroup of dMRE11 patients treated with IFL, an unexplained early increase in mortality but better long-term DFS than IFL-treated pMRE11 patients was observed. CONCLUSIONS: Analysis of this relatively small number of patients and events showed that the dMRE11 marker predicts better prognosis independent of treatment in the long-term. In subgroup analyses, dMRE11 patients treated with irinotecan exhibited unexplained short-term mortality. MRE11 status is readily assayed and may therefore prove to be a useful prognostic marker, provided that the results reported here for a relatively small number of patients can be generalized in independent analyses of larger numbers of samples. TRIAL REGISTRATION: NCT00003835.

  9. Subsurface Signature of the Internal Wave Field Radiated by Submerged High Reynolds Number Stratified Wakes


    parametric subharmonic instability. 15. SUBJECT TERMS Stratified turbulent wakes, high Reynolds numbers, internal waves, nonlinear effects, harmonics, mean...beam and the potential for parametric subharmonic instability. In all these efforts, a uniform linear stratification was considered. A subset of our...found for all simulated waves. c) For sufficiently high-amplitude beams, a parametric subharmonic instability is observed after a long enough time

  10. LTRMP Fisheries Data - Stratified Random and Fixed Site Sampling

    U.S. Geological Survey, Department of the Interior — The Long Term Resource Monitoring Programs (LTRMP) annual fish monitoring began on the Upper Mississippi and Illinois Rivers in 1989. During the first two years...

  11. The Risk-Stratified Osteoporosis Strategy Evaluation study (ROSE)

    Rubin, Katrine Hass; Holmberg, Teresa; Rothmann, Mette Juel


    The risk-stratified osteoporosis strategy evaluation study (ROSE) is a randomized prospective population-based study investigating the effectiveness of a two-step screening program for osteoporosis in women. This paper reports the study design and baseline characteristics of the study population....... 35,000 women aged 65-80 years were selected at random from the population in the Region of Southern Denmark and-before inclusion-randomized to either a screening group or a control group. As first step, a self-administered questionnaire regarding risk factors for osteoporosis based on FRAX......(®) was issued to both groups. As second step, subjects in the screening group with a 10-year probability of major osteoporotic fractures ≥15 % were offered a DXA scan. Patients diagnosed with osteoporosis from the DXA scan were advised to see their GP and discuss pharmaceutical treatment according to Danish...

  12. CUDA Enabled Graph Subset Examiner


    Finding Godsil-McKay switching sets in graphs is one way to demonstrate that a specific graph is not determined by its spectrum--the eigenvalues of its adjacency matrix. An important area of active research in pure mathematics is determining which graphs are determined by their spectra, i.e. when the spectrum of the adjacency matrix uniquely determines the underlying graph. We are interested in exploring the spectra of graphs in the Johnson scheme and specifically seek to determine which of these graphs are determined by their spectra. Given a graph G, a Godsil-McKay switching set is an induced subgraph H on 2k vertices with the following properties: I) H is regular, ii) every vertex in G/H is adjacent to either 0, k, or 2k vertices of H, and iii) at least one vertex in G/H is adjacent to k vertices in H. The software package examines each subset of a user specified size to determine whether or not it satisfies those 3 conditions. The software makes use of the massive parallel processing power of CUDA enabled GPUs. It also exploits the vertex transitivity of graphs in the Johnson scheme by reasoning that if G has a Godsil-McKay switching set, then it has a switching set which includes vertex 1. While the code (in its current state) is tuned to this specific problem, the method of examining each induced subgraph of G can be easily re-written to check for any user specified conditions on the subgraphs and can therefore be used much more broadly.

  13. Almost Chebyshev set with respect to bounded subsets

    李冲; 王兴华


    The uniqueness and existence of restricted Chebyshev center with respect to arbitrary subset are investigated. The concept of almost Chebyshev sets with respect to bounded subsets is introduced. It is proved that each closed subset in a reflexive locally uniformly convex (uniformly convex, respectively) Banach space is an almost Chebyshev subset with respect to compact convex subsets (bounded convex subsets and bounded subsets, respectively).

  14. A BK inequality for randomly drawn subsets of fixed size

    Berg, J van den


    The BK inequality, proved by van den Berg and Kesten \\cite{BeKe85}, says that, for product measures on $\\Om := \\{0,1\\}^n$, the probability that two increasing events $A$ and $B$ 'occur disjointly' is smaller than or equal to the product of the two individual probabilities. Their conjecture that this holds for {\\em all} events was proved by Reimer \\cite{Re00}. In spite of Reimer's work, several fundamental problems in this area remained open. For instance, although it is easy to see that non-product measures can not satisfy the above inequality for all events, there are several such measures on $\\Om$ which, intuitively, should satisfy the inequality for all increasing events. One of the most 'natural' candidates is what we call here the $k$-out-of-$n$ measure, the measure assigning equal probabilities to all elements of $\\Om$ with exactly $k$ 1's (and probability 0 to all other elements). The main contribution of this paper is a proof for these measures. Remarkably, although the $k$-out-of-$n$ measures are not...

  15. Stably stratified magnetized stars in general relativity

    Yoshida, Shijun; Shibata, Masaru


    We construct magnetized stars composed of a fluid stably stratified by entropy gradients in the framework of general relativity, assuming ideal magnetohydrodynamics and employing a barotropic equation of state. We first revisit basic equations for describing stably-stratified stationary axisymmetric stars containing both poloidal and toroidal magnetic fields. As sample models, the magnetized stars considered by Ioka and Sasaki (2004), inside which the magnetic fields are confined, are modified to the ones stably stratified. The magnetized stars newly constructed in this study are believed to be more stable than the existing relativistic models because they have both poloidal and toroidal magnetic fields with comparable strength, and magnetic buoyancy instabilities near the surface of the star, which can be stabilized by the stratification, are suppressed.

  16. Some Properties of α-Subsets

    LONG Xi-qing; XU Yang; GU Xiu-mei


    In this paper, the concept of α - subsets is introduced in a lattice implication algebra and some properties are discussed. Then we prove that an α- subset is a lattice ideal of L. In the end, we discuss the properties of annihilator.

  17. A Submodularity Framework for Data Subset Selection


    evaluate novel data subset selection algorithms that are based on the framework of submodular functions, a class of mathematical functions that selection to the problems of automatic speech recognition (ASR) and machine translation (MT). Data subset selection algorithms were used to

  18. Development of a synchronous subset of AADL

    Filali, Mamoun; Lawall, Julia


    We study the definition and the mapping of an AADL subset: the so called synchronous subset. We show that the data port protocol used for delayed and immediate connections between periodic threads can be interpreted in a  synchronous way. In this paper, we formalize this interpretation and study...

  19. Digital Image Correlation with Dynamic Subset Selection

    Hassan, Ghulam Mubashar; MacNish, Cara; Dyskin, Arcady; Shufrin, Igor


    The quality of the surface pattern and selection of subset size play a critical role in achieving high accuracy in Digital Image Correlation (DIC). The subset size in DIC is normally selected by testing different subset sizes across the entire image, which is a laborious procedure. This also leads to the problem that the worst region of the surface pattern influences the performance of DIC across the entire image. In order to avoid these limitations, a Dynamic Subset Selection (DSS) algorithm is proposed in this paper to optimize the subset size for each point in an image before optimizing the correlation parameters. The proposed DSS algorithm uses the local pattern around the point of interest to calculate a parameter called the Intensity Variation Ratio (Λ), which is used to optimize the subset size. The performance of the DSS algorithm is analyzed using numerically generated images and is compared with the results of traditional DIC. Images obtained from laboratory experiments are also used to demonstrate the utility of the DSS algorithm. Results illustrate that the DSS algorithm provides a better alternative to subset size "guessing" and finds an appropriate subset size for each point of interest according to the local pattern.

  20. Thermals in stratified regions of the ISM

    Rodriguez-Gonzalez, Ary


    We present a model of a "thermal" (i.e., a hot bubble) rising within an exponentially stratified region of the ISM. This model includes terms representing the ram pressure braking and the entrainment of environmental gas into the thermal. We then calibrate the free parameters associated with these two terms through a comparison with 3D numerical simulations of a rising bubble. Finally, we apply our "thermal" model to the case of a hot bubble produced by a SN within the stratified ISM of the Galactic disk.

  1. On Stratified Vortex Motions under Gravity.


    AD-A156 930 ON STRATIFIED VORTEX MOTIONS UNDER GRAVITY (U) NAVAL i/i RESEARCH LAB WASHINGTON DC Y T FUNG 20 JUN 85 NRL-MIR-5564 UNCLASSIFIED F/G 20/4...Under Gravity LCn * Y. T. Fung Fluid Dynamics Branch - Marine Technologyv Division June 20, 1985 SO Cyk. NAVAL RESEARCH LABORATORY Washington, D.C...DN880-019 TITLE (Include Security Classification) On Stratified Vortex Motions Under Gravity 12 PERSONAL AUTHOR(S) Funa, Y.T. 13a. TYPE OF REPORT 13b

  2. Mixing by microorganisms in stratified fluids

    Wagner, Gregory L; Lauga, Eric


    We examine the vertical mixing induced by the swimming of microorganisms at low Reynolds and P\\'eclet numbers in a stably stratified ocean, and show that the global contribution of oceanic microswimmers to vertical mixing is negligible. We propose two approaches to estimating the mixing efficiency, $\\eta$, or the ratio of the rate of potential energy creation to the total rate-of-working on the ocean by microswimmers. The first is based on scaling arguments and estimates $\\eta$ in terms of the ratio between the typical organism size, $a$, and an intrinsic length scale for the stratified flow, $\\ell = \\left ( \


    A. Rodríguez-González


    Full Text Available We present a model of a “thermal” (i.e., a hot bubble rising within an exponentially stratified region of the ISM. This model includes terms representing the ram pressure braking and the entrainment of environmental gas into the thermal. We then calibrate the free parameters associated with these two terms through a comparison with 3D numerical simulations of a rising bubble. Finally, we apply our “thermal” model to the case of a hot bubble produced by a SN within the stratified ISM of the Galactic disk.

  4. Turbulent Mixing in Stably Stratified Flows


    Liege Colloquium on Ocean Hydrodynamics, volume 46, page 19889898. Elsevier, 1987. R. M. Kerr. Higher-order derivative correlations and the alignment of...19th International Liege Colloquium on Ocean Hydrodynamics, volume 46, pages 3-9. Elsevier, 1988. P. Meunier and G. Spedding. Stratified propelled

  5. Nitrogen transformations in stratified aquatic microbial ecosystems

    Revsbech, Niels Peter; Risgaard-Petersen, N.; Schramm, Andreas


    Abstract  New analytical methods such as advanced molecular techniques and microsensors have resulted in new insights about how nitrogen transformations in stratified microbial systems such as sediments and biofilms are regulated at a µm-mm scale. A large and ever-expanding knowledge base about n...

  6. Subset selection circumvents the square root law

    Craver, Scott; Yu, Jun


    The square root law holds that acceptable embedding rate is sublinear in the cover size, specifically O(square root of n), in order to prevent detection as the warden's data and thus detector power increases. One way to transcend this law, at least in the, is to restrict the cover to a chosen subset whose distribution is close to that of altered data. Embedding is then performed on this subset; this replaces the problem of finding a small enough subset to evade detection with the problem of finding a large enough subset that possesses a desired type distribution. We show that one can find such a subset of size asymptotically proportional to n rather than the square root of n. This works in the case of both replacement and tampering: Even if the distribution of tampered data depends on the distribution of cover data, one can find a fixed point in the probability simplex such that cover data of that distribution yields stego data of the same distribution. While the transmission of a subset is not allowed, this is no impediment: wet paper codes can be used, else in the worst case a maximal desirable subset can be computed from the cover by both sender and receiver without communication of side information.

  7. Variable and subset selection in PLS regression

    Høskuldsson, Agnar


    The purpose of this paper is to present some useful methods for introductory analysis of variables and subsets in relation to PLS regression. We present here methods that are efficient in finding the appropriate variables or subset to use in the PLS regression. The general conclusion...... is that variable selection is important for successful analysis of chemometric data. An important aspect of the results presented is that lack of variable selection can spoil the PLS regression, and that cross-validation measures using a test set can show larger variation, when we use different subsets of X, than...


    YANG Yong-yu; LIU Xin-wei; YANG Fan


    The curve of relationship between fatigue crack growth rate and the stress strength factor amplitude represented an important fatigue property in designing of damage tolerance limits and predicting life of metallic component parts. In order to have a morereasonable use of testing data, samples from population were stratified suggested by the stratified random sample model (SRAM). The data in each stratum corresponded to the same experiment conditions. A suitable weight was assigned to each stratified sample according to the actual working states of the pressure vessel, so that the estimation of fatigue crack growth rate equation was more accurate for practice. An empirical study shows that the SRAM estimation by using fatigue crack growth rate data from different stoves is obviously better than the estimation from simple random sample model.


    H.K. EI-Sayied


    Invex bodies represent an important class of bodies which are considered as ageneralization of convex bodies. In this article, the author studies the supporting for thisclass of bodies as well as the separating subsets of two bodies.


    徐瑞; 张加兴; 苏成


    基于动力响应显式表达式,时域显式随机模拟法可以通过减少单次样本计算时间有效提高动力可靠度的计算效率.然而,对于小失效概率问题,由于需要大量次样本计算,该法的计算量仍相当可观.为了克服上述困难,在时域显式随机模拟法基础上引入子集模拟法的基本思想,把小失效概率表示为一系列较大的条件概率的乘积,其中各条件概率采用时域显式随机模拟法计算,条件域内的样本采用Metropolis-Hastings抽样方法生成,从而实现了减少随机模拟所需的样本数,进一步提高了计算效率.算例结果表明改进的方法具有更高的计算效率,更适用于小失效概率和多自由度结构的动力可靠度问题.%Based on the explicit expressions of dynamic responses,a dime-domain explicit formulation random simulation method was developed to remarkably improve the efficiency of a dynamic reliability analysis by reducing single sample calculation time.However,for small failure probability events,the computational cost is still quite considerable because of the requirement of a large number of samples.To overcome above difficulty,a basic idea of subset simulation that expressing a small failure probability as a product of lager conditional probabilities is introduced to the dime-domain explicit formulation random simulation method to further lower the sample number.Among them,the conditional probabilities are obtained by the dime-domain explicit formulation random simulation method,and the samples required for obtaining the conditional probabilities are generated by Metropolis-Hastings algorithm.Numerical results show that the improved method has a higher computational efficiency and especially applicable for the dynamic reliability problems of small failure probability and for multi-degree-of-freedom structures.

  11. Curvatures for Parameter Subsets in Nonlinear Regression


    The relative curvature measures of nonlinearity proposed by Bates and Watts (1980) are extended to an arbitrary subset of the parameters in a normal, nonlinear regression model. In particular, the subset curvatures proposed indicate the validity of linearization-based approximate confidence intervals for single parameters. The derivation produces the original Bates-Watts measures directly from the likelihood function. When the intrinsic curvature is negligible, the Bates-Watts parameter-effec...

  12. Design and simulation of stratified probability digital receiver with application to the multipath communication

    Deal, J. H.


    One approach to the problem of simplifying complex nonlinear filtering algorithms is through using stratified probability approximations where the continuous probability density functions of certain random variables are represented by discrete mass approximations. This technique is developed in this paper and used to simplify the filtering algorithms developed for the optimum receiver for signals corrupted by both additive and multiplicative noise.

  13. Drainage in a model stratified porous medium

    Datta, Sujit S; 10.1209/0295-5075/101/14002


    We show that when a non-wetting fluid drains a stratified porous medium at sufficiently small capillary numbers Ca, it flows only through the coarsest stratum of the medium; by contrast, above a threshold Ca, the non-wetting fluid is also forced laterally, into part of the adjacent, finer strata. The spatial extent of this partial invasion increases with Ca. We quantitatively understand this behavior by balancing the stratum-scale viscous pressure driving the flow with the capillary pressure required to invade individual pores. Because geological formations are frequently stratified, we anticipate that our results will be relevant to a number of important applications, including understanding oil migration, preventing groundwater contamination, and sub-surface CO$_{2}$ storage.

  14. Stably Stratified Flow in a Shallow Valley

    Mahrt, L.


    Stratified nocturnal flow above and within a small valley of approximately 12-m depth and a few hundred metres width is examined as a case study, based on a network of 20 sonic anemometers and a central 20-m tower with eight levels of sonic anemometers. Several regimes of stratified flow over gentle topography are conceptually defined for organizing the data analysis and comparing with the existing literature. In our case study, a marginal cold pool forms within the shallow valley in the early evening but yields to larger ambient wind speeds after a few hours, corresponding to stratified terrain-following flow where the flow outside the valley descends to the valley floor. The terrain-following flow lasts about 10 h and then undergoes transition to an intermittent marginal cold pool towards the end of the night when the larger-scale flow collapses. During this 10-h period, the stratified terrain-following flow is characterized by a three-layer structure, consisting of a thin surface boundary layer of a few metres depth on the valley floor, a deeper boundary layer corresponding to the larger-scale flow, and an intermediate transition layer with significant wind-directional shear and possible advection of lee turbulence that is generated even for the gentle topography of our study. The flow in the valley is often modulated by oscillations with a typical period of 10 min. Cold events with smaller turbulent intensity and duration of tens of minutes move through the observational domain throughout the terrain-following period. One of these events is examined in detail.

  15. Health-related quality of life predictors during medical residency in a random, stratified sample of residents Preditores de qualidade de vida relacionada à saúde durante a residência médica em uma amostra randomizada e estratificada de médicos residentes

    Paula Costa Mosca Macedo


    Full Text Available OBJECTIVE: To evaluate the quality of life during the first three years of training and identify its association with sociodemographicoccupational characteristics, leisure time and health habits. METHOD: A cross-sectional study with a random sample of 128 residents stratified by year of training was conducted. The Medical Outcome Study -short form 36 was administered. Mann-Whitney tests were carried out to compare percentile distributions of the eight quality of life domains, according to sociodemographic variables, and a multiple linear regression analysis was performed, followed by a validity checking for the resulting models. RESULTS: The physical component presented higher quality of life medians than the mental component. Comparisons between the three years showed that in almost all domains the quality of life scores of the second year residents were higher than the first year residents (p OBJETIVO: Avaliar a qualidade de vida do médico residente durante os três anos do treinamento e identificar sua associação com as características sociodemográficas-ocupacionais, tempo de lazer e hábitos de saúde. MÉTODO: Foi realizado um estudo transversal com amostra randomizada de 128 residentes, estratificada por ano de residência. O Medical Outcome Study-Short Form 36 foi aplicado; as distribuições percentis dos domínios de qualidade de vida de acordo com variáveis sociodemográficas foram analisadas pelo teste de Mann-Whitney e regressão linear múltipla, bem como estudo de validação pós-regressão. RESULTADOS: O componente físico da qualidade de vida apresentou medianas mais altas do que o mental. Comparações entre os três anos mostraram que quase todos os domínios de qualidade de vida tiveram escores maiores no segundo do que no primeiro ano (p < 0,01; em relação ao componente mental observamos maiores escores no terceiro ano do que nos demais (p < 0,01. Preditores de maior qualidade de vida foram: estar no segundo ou

  16. Multi Dimensional CTL and Stratified Datalog

    Theodore Andronikos


    Full Text Available In this work we define Multi Dimensional CTL (MD-CTL in short by extending CTL which is thedominant temporal specification language in practice. The need for Multi Dimensional CTL is mainlydue to the advent of semi-structured data. The common path nature of CTL and XPath which provides asuitable model for semi-structured data, has caused the emergence of work on specifying a relation amongthem aiming at exploiting the nice properties of CTL. Although the advantages of such an approach havealready been noticed [36, 26, 5], no formal definition of MD-CTL has been given. The goal of this workis twofold; a we define MD-CTL and prove that the “nice” properties of CTL (linear model checking andbounded model property transfer also to MD-CTL, b we establish new results on stratified Datalog. Inparticular, we define a fragment of stratified Datalog called Multi Branching Temporal (MBT in shortprograms that has the same expressive power as MD-CTL. We prove that by devising a linear translationbetween MBT and MD-CTL. We actually give the exact translation rules for both directions. We furtherbuild on this relation to prove that query evaluation is linear and checking satisfiability, containment andequivalence are EXPTIME–complete for MBT programs. The class MBT is the largest fragment of stratifiedDatalog for which such results exist in the literature.

  17. Thermal mixing in a stratified environment

    Kraemer, Damian; Cotel, Aline


    Laboratory experiments of a thermal impinging on a stratified interface have been performed. The thermal was released from a cylindrical reservoir located at the bottom of a Lucite tank. The stratified interface was created by filling the tank with two different saline solutions. The density of the lower layer is greater than that of the upper layer and the thermal fluid, thereby creating a stable stratification. A pH indicator, phenolphthalein, is used to visualize and quantify the amount of mixing produced by the impingement of the thermal at the interface. The upper layer contains a mixture of water, salt and sodium hydroxide. The thermal fluid is composed of water, sulfuric acid and phenolphthalein. When the thermal entrains and mixes fluid from the upper layer, a chemical reaction takes place, and the resulting mixed fluid is now visible. The ratio of base to acid, called the equivalence ratio, was varied throughout the experiments, as well as the Richardson number. The Richardson number is the ratio of potential to kinetic energy, and is based on the thermal quantities at the interface. Results indicate that the amount of mixing produced is proportional to the Richardson number raised to the -3/2 power. Previous experiments (Zhang and Cotel 1999) revealed that the entrainment rate of a thermal in a stratified environment follows the same power law.

  18. Sequential stratified sampling belief propagation for multiple targets tracking


    Rather than the difficulties of highly non-linear and non-Gaussian observation process and the state distribution in single target tracking, the presence of a large, varying number of targets and their interactions place more challenge on visual tracking. To overcome these difficulties, we formulate multiple targets tracking problem in a dynamic Markov network which consists of three coupled Markov random fields that model the following: a field for joint state of multi-target, one binary process for existence of individual target, and another binary process for occlusion of dual adjacent targets. By introducing two robust functions, we eliminate the two binary processes, and then apply a novel version of belief propagation called sequential stratified sampling belief propagation algorithm to obtain the maximum a posteriori (MAP) estimation in the dynamic Markov network. By using stratified sampler, we incorporate bottom-up information provided by a learned detector (e.g. SVM classifier) and belief information for the messages updating. Other low-level visual cues (e.g. color and shape) can be easily incorporated in our multi-target tracking model to obtain better tracking results. Experimental results suggest that our method is comparable to the state-of-the-art multiple targets tracking methods in several test cases.




    Khoshnevisan et al. (2007) proposed a general family of estimators for population mean using known value of some population parameters in simple random sampling. The objective of this paper is to propose a family of combined-type estimators in stratified random sampling adapting the family of estimators proposed by Khoshnevisan et al. (2007) under non-response. The properties of proposed family have been discussed. We have also obtained the expressions for optimum sampl...


    Chaudhary, Manoj K.; Rajesh Singh; Rakesh K. Shukla; Mukesh Kumar; Florentin Smarandache


    Khoshnevisan et al. (2007) proposed a general family of estimators for population mean using known value of some population parameters in simple random sampling. The objective of this paper is to propose a family of combined-type estimators in stratified random sampling adapting the family of estimators proposed by Khoshnevisan et al. (2007) under non-response. The properties of proposed family have been discussed. We have also obtained the expressions for optimum sample sizes of the strata i...

  1. Improved Subset Autoregression: With R Package

    A. I. McLeod


    Full Text Available The FitAR R (R Development Core Team 2008 package that is available on the Comprehensive R Archive Network is described. This package provides a comprehensive approach to fitting autoregressive and subset autoregressive time series. For long time series with complicated autocorrelation behavior, such as the monthly sunspot numbers, subset autoregression may prove more feasible and/or parsimonious than using AR or ARMA models. The two principal functions in this package are SelectModel and FitAR for automatic model selection and model fitting respectively. In addition to the regular autoregressive model and the usual subset autoregressive models (Tong 1977, these functions implement a new family of models. This new family of subset autoregressive models is obtained by using the partial autocorrelations as parameters and then selecting a subset of these parameters. Further properties and results for these models are discussed in McLeod and Zhang (2006. The advantages of this approach are that not only is an efficient algorithm for exact maximum likelihood implemented but that efficient methods are derived for selecting high-order subset models that may occur in massive datasets containing long time series. A new improved extended {BIC} criterion, {UBIC}, developed by Chen and Chen (2008 is implemented for subset model selection. A complete suite of model building functions for each of the three types of autoregressive models described above are included in the package. The package includes functions for time series plots, diagnostic testing and plotting, bootstrapping, simulation, forecasting, Box-Cox analysis, spectral density estimation and other useful time series procedures. As well as methods for standard generic functions including print, plot, predict and others, some new generic functions and methods are supplied that make it easier to work with the output from FitAR for bootstrapping, simulation, spectral density estimation and Box

  2. The fully nonlinear stratified geostrophic adjustment problem

    Coutino, Aaron; Stastna, Marek


    The study of the adjustment to equilibrium by a stratified fluid in a rotating reference frame is a classical problem in geophysical fluid dynamics. We consider the fully nonlinear, stratified adjustment problem from a numerical point of view. We present results of smoothed dam break simulations based on experiments in the published literature, with a focus on both the wave trains that propagate away from the nascent geostrophic state and the geostrophic state itself. We demonstrate that for Rossby numbers in excess of roughly 2 the wave train cannot be interpreted in terms of linear theory. This wave train consists of a leading solitary-like packet and a trailing tail of dispersive waves. However, it is found that the leading wave packet never completely separates from the trailing tail. Somewhat surprisingly, the inertial oscillations associated with the geostrophic state exhibit evidence of nonlinearity even when the Rossby number falls below 1. We vary the width of the initial disturbance and the rotation rate so as to keep the Rossby number fixed, and find that while the qualitative response remains consistent, the Froude number varies, and these variations are manifested in the form of the emanating wave train. For wider initial disturbances we find clear evidence of a wave train that initially propagates toward the near wall, reflects, and propagates away from the geostrophic state behind the leading wave train. We compare kinetic energy inside and outside of the geostrophic state, finding that for long times a Rossby number of around one-quarter yields an equal split between the two, with lower (higher) Rossby numbers yielding more energy in the geostrophic state (wave train). Finally we compare the energetics of the geostrophic state as the Rossby number varies, finding long-lived inertial oscillations in the majority of the cases and a general agreement with the past literature that employed either hydrostatic, shallow-water equation-based theory or

  3. Inverse scattering of dispersive stratified structures

    Skaar, Johannes


    We consider the inverse scattering problem of retrieving the structural parameters of a stratified medium consisting of dispersive materials, given knowledge of the complex reflection coefficient in a finite frequency range. It is shown that the inverse scattering problem does not have a unique solution in general. When the dispersion is sufficiently small, such that the time-domain Fresnel reflections have durations less than the round-trip time in the layers, the solution is unique and can be found by layer peeling. Numerical examples with dispersive and lossy media are given, demonstrating the usefulness of the method for e.g. THz technology.

  4. Topological Structures in Rotating Stratified Flows

    Redondo, J. M.; Carrillo, A.; Perez, E.


    Detailled 2D Particle traking and PIV visualizations performed on a series of large scale laboratory experiments at the Coriolis Platform of the SINTEF in Trondheim have revealed several resonances which scale on the Strouhal, the Rossby and the Richardson numbers. More than 100 experiments spanned a wide range of Rossby Deformation Radii and the topological structures (Parabolic /Eliptic /Hyperbolic) of the quasi-balanced stratified-rotating flows were studied when stirring (akin to coastal mixing) occured at a side of the tank. The strong asymetry favored by the total vorticity produces a wealth of mixing patterns.

  5. Subset specification of central serotonergic neurons

    Marten P Smidt


    Full Text Available The last decade the serotonin (5-hydroxytryptamine; 5-HT system has received enormous attention due to its role in regulation of behavior, exemplified by the discovery that increased 5-HT tone in the central nervous system is able to alleviate affective disorders. Here, we review the developmental processes, with a special emphasis on subset specification, leading to the formation of the 5-HT system in the brain. Molecular classification of 5-HT neuronal groups leads to the definition of two independent rostral groups positioned in rhombomere 1 and 2/3 and a caudal group in rhombomere 5-8. In addition, more disperse refinement of these subsets is present as shown by the selective expression of the 5-HT1A autoreceptor, indicating functional diversity between 5-HT subsets. The functional significance of the molecular coding differences is not well known and the molecular basis of described specific connectivity patterns remain to be elucidated. Recent developments in genetic lineage tracing models will provide these data and form a major step-up towards the full understanding of the importance of developmental programming and function of 5-HT neuronal subsets.

  6. Calibration with empirically weighted mean subset

    Øjelund, Henrik; Madsen, Henrik; Thyregod, Poul


    In this article a new calibration method called empirically weighted mean subset (EMS) is presented. The method is illustrated using spectral data. Using several near-infrared (NIR) benchmark data sets, EMS is compared to partial least-squares regression (PLS) and interval partial least-squares r......In this article a new calibration method called empirically weighted mean subset (EMS) is presented. The method is illustrated using spectral data. Using several near-infrared (NIR) benchmark data sets, EMS is compared to partial least-squares regression (PLS) and interval partial least...... is obtained by calculating the weighted mean of all coefficient vectors for subsets of the same size. The weighting is proportional to SSgamma-omega, where SSgamma is the residual sum of squares from a linear regression with subset gamma and omega is a weighting parameter estimated using cross......-validation. This construction of the weighting implies that even if some coefficients will become numerically small, none will become exactly zero. An efficient algorithm has been implemented in MATLAB to calculate the EMS solution and the source code has been made available on the Internet....

  7. Feature subset selection based on relevance

    Wang, Hui; Bell, David; Murtagh, Fionn

    In this paper an axiomatic characterisation of feature subset selection is presented. Two axioms are presented: sufficiency axiom—preservation of learning information, and necessity axiom—minimising encoding length. The sufficiency axiom concerns the existing dataset and is derived based on the following understanding: any selected feature subset should be able to describe the training dataset without losing information, i.e. it is consistent with the training dataset. The necessity axiom concerns the predictability and is derived from Occam's razor, which states that the simplest among different alternatives is preferred for prediction. The two axioms are then restated in terms of relevance in a concise form: maximising both the r( X; Y) and r( Y; X) relevance. Based on the relevance characterisation, four feature subset selection algorithms are presented and analysed: one is exhaustive and the remaining three are heuristic. Experimentation is also presented and the results are encouraging. Comparison is also made with some well-known feature subset selection algorithms, in particular, with the built-in feature selection mechanism in C4.5.

  8. Ordered-subset analysis of savant skills in autism for 15q11-q13.

    Ma, D Q; Jaworski, J; Menold, M M; Donnelly, S; Abramson, R K; Wright, H H; Delong, G R; Gilbert, J R; Pericak-Vance, M A; Cuccaro, Michael L


    Autism is a complex disorder characterized by genetic and phenotypic heterogeneity. Analysis of phenotypically homogeneous subtypes has been used to both confirm and narrow potential autism linkage regions such as the chromosomal region 15q11-q13. Increased evidence for linkage in this region had been found in a subgroup of 21 autism families (total families = 94) stratified based on a savant skill factor (SSF) from the Autism Diagnostic Interview, Revised (ADI-R). We examined the savant phenotypic finding in our sample of 91 multiplex autism families. Using two-point parametric analysis in stratification with a cutoff point of a savant skill score of 0.16, our families failed to demonstrate linkage to 15q11-q13. In addition, ordered subset analysis (OSA) using SSF as a covariate also failed to show evidence for linkage. Our findings do not support savant skills as an informative phenotypic subset for linkage in our sample.

  9. Nonlinear gravity-wave interactions in stratified turbulence

    Remmel, Mark; Sukhatme, Jai; Smith, Leslie M.


    To investigate the dynamics of gravity waves in stratified Boussinesq flows, a model is derived that consists of all three-gravity-wave-mode interactions (the GGG model), excluding interactions involving the vortical mode. The GGG model is a natural extension of weak turbulence theory that accounts for exact three-gravity-wave resonances. The model is examined numerically by means of random, large-scale, high-frequency forcing. An immediate observation is a robust growth of the so-called vertically sheared horizontal flow (VSHF). In addition, there is a forward transfer of energy and equilibration of the nonzero-frequency (sometimes called "fast") gravity-wave modes. These results show that gravity-wave-mode interactions by themselves are capable of systematic interscale energy transfer in a stratified fluid. Comparing numerical simulations of the GGG model and the full Boussinesq system, for the range of Froude numbers ( Fr) considered (0.05 ≤ Fr ≤ 1), in both systems the VSHF is hardest to resolve. When adequately resolved, VSHF growth is more vigorous in the GGG model. Furthermore, a VSHF is observed to form in milder stratification scenarios in the GGG model than the full Boussinesq system. Finally, fully three-dimensional nonzero-frequency gravity-wave modes equilibrate in both systems and their scaling with vertical wavenumber follows similar power-laws. The slopes of the power-laws obtained depend on Fr and approach -2 (from above) at Fr = 0.05, which is the strongest stratification that can be properly resolved with our computational resources.

  10. Stratified growth in Pseudomonas aeruginosa biofilms

    Werner, E.; Roe, F.; Bugnicourt, A.;


    In this study, stratified patterns of protein synthesis and growth were demonstrated in Pseudomonas aeruginosa biofilms. Spatial patterns of protein synthetic activity inside biofilms were characterized by the use of two green fluorescent protein (GFP) reporter gene constructs. One construct...... carried an isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible gfpmut2 gene encoding a stable GFP. The second construct carried a GFP derivative, gfp-AGA, encoding an unstable GFP under the control of the growth-rate-dependent rrnBp(1) promoter. Both GFP reporters indicated that active protein...... of oxygen limitation in the biofilm. Oxygen microelectrode measurements showed that oxygen only penetrated approximately 50 mum into the biofilm. P. aeruginosa was incapable of anaerobic growth in the medium used for this investigation. These results show that while mature P. aeruginosa biofilms contain...

  11. Bayesian Stratified Sampling to Assess Corpus Utility

    Hochberg, J; Thomas, T; Hall, S; Hochberg, Judith; Scovel, Clint; Thomas, Timothy; Hall, Sam


    This paper describes a method for asking statistical questions about a large text corpus. We exemplify the method by addressing the question, "What percentage of Federal Register documents are real documents, of possible interest to a text researcher or analyst?" We estimate an answer to this question by evaluating 200 documents selected from a corpus of 45,820 Federal Register documents. Stratified sampling is used to reduce the sampling uncertainty of the estimate from over 3100 documents to fewer than 1000. The stratification is based on observed characteristics of real documents, while the sampling procedure incorporates a Bayesian version of Neyman allocation. A possible application of the method is to establish baseline statistics used to estimate recall rates for information retrieval systems.

  12. Clustering of floating particles in stratified turbulence

    Boffetta, Guido; de Lillo, Filippo; Musacchio, Stefano; Sozza, Alessandro


    We study the dynamics of small floating particles transported by stratified turbulence in presence of a mean linear density profile as a simple model for the confinement and the accumulation of plankton in the ocean. By means of extensive direct numerical simulations we investigate the statistical distribution of floaters as a function of the two dimensionless parameters of the problem. We find that vertical confinement of particles is mainly ruled by the degree of stratification, with a weak dependency on the particle properties. Conversely, small scale fractal clustering, typical of non-neutral particles in turbulence, depends on the particle relaxation time and is only weakly dependent on the flow stratification. The implications of our findings for the formation of thin phytoplankton layers are discussed.

  13. On turbulence in a stratified environment

    Sarkar, Sutanu


    John Lumley, motivated by atmospheric observations, made seminal contributions to the statistical theory (Lumley and Panofsky 1964, Lumley 1964) and second-order modeling (Zeman and Lumley 1976) of turbulence in the environment. Turbulent processes in the ocean share many features with the atmosphere, e.g., shear, stratification, rotation and rough topography. Results from direct and large eddy simulations of two model problems will be used to illustrate some of the features of turbulence in a stratified environment. The first problem concerns a shear layer in nonuniform stratification, a situation typical of both the atmosphere and the ocean. The second problem, considered to be responsible for much of the turbulent mixing that occurs in the ocean interior, concerns topographically generated internal gravity waves. Connections will be made to data taken during observational campaigns in the ocean.

  14. Stratified scaffold design for engineering composite tissues.

    Mosher, Christopher Z; Spalazzi, Jeffrey P; Lu, Helen H


    A significant challenge to orthopaedic soft tissue repair is the biological fixation of autologous or allogeneic grafts with bone, whereby the lack of functional integration between such grafts and host bone has limited the clinical success of anterior cruciate ligament (ACL) and other common soft tissue-based reconstructive grafts. The inability of current surgical reconstruction to restore the native fibrocartilaginous insertion between the ACL and the femur or tibia, which minimizes stress concentration and facilitates load transfer between the soft and hard tissues, compromises the long-term clinical functionality of these grafts. To enable integration, a stratified scaffold design that mimics the multiple tissue regions of the ACL interface (ligament-fibrocartilage-bone) represents a promising strategy for composite tissue formation. Moreover, distinct cellular organization and phase-specific matrix heterogeneity achieved through co- or tri-culture within the scaffold system can promote biomimetic multi-tissue regeneration. Here, we describe the methods for fabricating a tri-phasic scaffold intended for ligament-bone integration, as well as the tri-culture of fibroblasts, chondrocytes, and osteoblasts on the stratified scaffold for the formation of structurally contiguous and compositionally distinct regions of ligament, fibrocartilage and bone. The primary advantage of the tri-phasic scaffold is the recapitulation of the multi-tissue organization across the native interface through the layered design. Moreover, in addition to ease of fabrication, each scaffold phase is similar in polymer composition and therefore can be joined together by sintering, enabling the seamless integration of each region and avoiding delamination between scaffold layers.

  15. Stratified sampling design based on data mining.

    Kim, Yeonkook J; Oh, Yoonhwan; Park, Sunghoon; Cho, Sungzoon; Park, Hayoung


    To explore classification rules based on data mining methodologies which are to be used in defining strata in stratified sampling of healthcare providers with improved sampling efficiency. We performed k-means clustering to group providers with similar characteristics, then, constructed decision trees on cluster labels to generate stratification rules. We assessed the variance explained by the stratification proposed in this study and by conventional stratification to evaluate the performance of the sampling design. We constructed a study database from health insurance claims data and providers' profile data made available to this study by the Health Insurance Review and Assessment Service of South Korea, and population data from Statistics Korea. From our database, we used the data for single specialty clinics or hospitals in two specialties, general surgery and ophthalmology, for the year 2011 in this study. Data mining resulted in five strata in general surgery with two stratification variables, the number of inpatients per specialist and population density of provider location, and five strata in ophthalmology with two stratification variables, the number of inpatients per specialist and number of beds. The percentages of variance in annual changes in the productivity of specialists explained by the stratification in general surgery and ophthalmology were 22% and 8%, respectively, whereas conventional stratification by the type of provider location and number of beds explained 2% and 0.2% of variance, respectively. This study demonstrated that data mining methods can be used in designing efficient stratified sampling with variables readily available to the insurer and government; it offers an alternative to the existing stratification method that is widely used in healthcare provider surveys in South Korea.

  16. Information content of household-stratified epidemics

    T.M. Kinyanjui


    Full Text Available Household structure is a key driver of many infectious diseases, as well as a natural target for interventions such as vaccination programs. Many theoretical and conceptual advances on household-stratified epidemic models are relatively recent, but have successfully managed to increase the applicability of such models to practical problems. To be of maximum realism and hence benefit, they require parameterisation from epidemiological data, and while household-stratified final size data has been the traditional source, increasingly time-series infection data from households are becoming available. This paper is concerned with the design of studies aimed at collecting time-series epidemic data in order to maximize the amount of information available to calibrate household models. A design decision involves a trade-off between the number of households to enrol and the sampling frequency. Two commonly used epidemiological study designs are considered: cross-sectional, where different households are sampled at every time point, and cohort, where the same households are followed over the course of the study period. The search for an optimal design uses Bayesian computationally intensive methods to explore the joint parameter-design space combined with the Shannon entropy of the posteriors to estimate the amount of information in each design. For the cross-sectional design, the amount of information increases with the sampling intensity, i.e., the designs with the highest number of time points have the most information. On the other hand, the cohort design often exhibits a trade-off between the number of households sampled and the intensity of follow-up. Our results broadly support the choices made in existing epidemiological data collection studies. Prospective problem-specific use of our computational methods can bring significant benefits in guiding future study designs.

  17. Lymphocyte subsets in healthy Malawians: implications for immunologic assessment of HIV infection in Africa.

    Mandala, Wilson L; MacLennan, Jenny M; Gondwe, Esther N; Ward, Steven A; Molyneux, Malcolm E; MacLennan, Calman A


    CD4(+)T lymphocyte measurements are the most important indicator of mortality in HIV-infected individuals in resource-limited settings. There is currently a lack of comprehensive immunophenotyping data from African populations to guide the immunologic assessment of HIV infection. To quantify variation in absolute and relative lymphocyte subsets with age in healthy Malawians. Lymphocyte subsets in peripheral blood of 539 healthy HIV-uninfected Malawians stratified by age were enumerated by flow cytometry. B and T-lymphocyte and T-lymphocyte subset absolute concentrations peaked in early childhood then decreased to adult levels, whereas lymphocyte subset proportions demonstrated much less variation with age. Adult lymphocyte subsets were similar to those in developed countries. In contrast, high B-lymphocyte and CD8(+)T-lymphocyte levels among children under 2 years, relative to those in developed countries, resulted in low CD4(+)T-lymphocyte percentages that varied little between 0 and 5 years (35% to 39%). The CD4(+)T-lymphocyte percentages in 35% of healthy children under 1 year and 18% of children age 1 to 3 years were below the World Health Organization threshold defining immunodeficiency in HIV-infected children in resource-limited settings. Thirteen percent of healthy children under 18 months old had a CD4:CD8T-lymphocyte ratio <1.0, which is commonly associated with HIV infection. All immunologic parameters except absolute natural killer lymphocyte concentration varied significantly with age, and percentage and overall absolute CD4(+)T-lymphocyte counts were higher in females than males. Although lymphocyte subsets in Malawian adults are similar to those from developed countries, CD4(+)T-lymphocyte percentages in young children are comparatively low. These findings need to be considered when assessing the severity of HIV-related immunodeficiency in African children under 3 years. Copyright 2010 American Academy of Allergy, Asthma & Immunology. Published by

  18. Product-free subsets of profinite groups

    Bardestani, Mohammad


    Gowers in his paper on quasirandom groups studies a question of Babai and Sos asking whether there exists a constant $c > 0$ such that every finite group $G$ has a product-free subset of size at least $c|G|$. Answering the question negatively, he proves that for sufficiently large prime $p$, the group $\\mathrm{PSL}_2(\\mathbb{F}_p)$ has no product-free subset of size $cn^{8/9}$, where $n$ is the order of $\\mathrm{PSL}_2(\\mathbb{F}_p)$. We will consider the problem for compact groups and in particular for profinite groups $\\SL_k(\\ZZ_p)$ obtain lower and upper exponential bounds for the supremal measure of the product-free sets. The proof involves establishing a lower bound for the dimension of non-trivial representations of the finite groups $\\SL_k(\\Z{p^n})$.

  19. Estimation of Finite Population Mean in Multivariate Stratified Sampling under Cost Function Using Goal Programming

    Atta Ullah


    Full Text Available In practical utilization of stratified random sampling scheme, the investigator meets a problem to select a sample that maximizes the precision of a finite population mean under cost constraint. An allocation of sample size becomes complicated when more than one characteristic is observed from each selected unit in a sample. In many real life situations, a linear cost function of a sample size nh is not a good approximation to actual cost of sample survey when traveling cost between selected units in a stratum is significant. In this paper, sample allocation problem in multivariate stratified random sampling with proposed cost function is formulated in integer nonlinear multiobjective mathematical programming. A solution procedure is proposed using extended lexicographic goal programming approach. A numerical example is presented to illustrate the computational details and to compare the efficiency of proposed compromise allocation.

  20. Magnetic flux concentrations from turbulent stratified convection

    Käpylä, P J; Kleeorin, N; Käpylä, M J; Rogachevskii, I


    (abridged) Context: The mechanisms that cause the formation of sunspots are still unclear. Aims: We study the self-organisation of initially uniform sub-equipartition magnetic fields by highly stratified turbulent convection. Methods: We perform simulations of magnetoconvection in Cartesian domains that are $8.5$-$24$ Mm deep and $34$-$96$ Mm wide. We impose either a vertical or a horizontal uniform magnetic field in a convection-driven turbulent flow. Results: We find that super-equipartition magnetic flux concentrations are formed near the surface with domain depths of $12.5$ and $24$ Mm. The size of the concentrations increases as the box size increases and the largest structures ($20$ Mm horizontally) are obtained in the 24 Mm deep models. The field strength in the concentrations is in the range of $3$-$5$ kG. The concentrations grow approximately linearly in time. The effective magnetic pressure measured in the simulations is positive near the surface and negative in the bulk of the convection zone. Its ...

  1. Monocyte subsets in myocardial infarction: A review.

    Arfvidsson, John; Ahlin, Fredrik; Vargas, Kris G; Thaler, Barbara; Wojta, Johann; Huber, Kurt


    Monocytes form an important part of the human innate immune system by taking part in inflammatory reactions. With time, monocytes have gained interest in the role they may play during the event of myocardial infarction (MI). The current paradigm suggests that monocytes consist of three subdivisions which differ in phenotypic and dynamic patterns after an MI. In the inflammation that ensues, the different subsets have been shown to have an impact on reparative processes and patient recovery. We searched Medline and Embase until April 5, 2016, for observational studies or clinical trials regarding monocyte functions and dynamics in MI. Apart from studies in humans, extensive work has been done in mice in an effort to understand the complex nature of monocyte dynamics. Animal models might add useful information on mapping these processes. The question still remains whether animal data can, to a certain degree, be extrapolated to monocyte functions during human MI. This review aims to summarize current available evidence on both mice and men with particular focus on the understanding of monocyte subsets dynamics and effects in human MI. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  2. Dense subsets of products of finite trees

    Dodos, Pandelis; Tyros, Konstantinos


    We prove a "uniform" version of the finite density Halpern-L\\"{a}uchli Theorem. Specifically, we say that a tree $T$ is homogeneous if it is uniquely rooted and there is an integer $b\\geq 2$, called the branching number of $T$, such that every $t\\in T$ has exactly $b$ immediate successors. We show the following. For every integer $d\\geq 1$, every $b_1,...,b_d\\in\\mathbb{N}$ with $b_i\\geq 2$ for all $i\\in\\{1,...,d\\}$, every integer $k\\meg 1$ and every real $0<\\epsilon\\leq 1$ there exists an integer $N$ with the following property. If $(T_1,...,T_d)$ are homogeneous trees such that the branching number of $T_i$ is $b_i$ for all $i\\in\\{1,...,d\\}$, $L$ is a finite subset of $\\mathbb{N}$ of cardinality at least $N$ and $D$ is a subset of the level product of $(T_1,...,T_d)$ satisfying \\[|D\\cap \\big(T_1(n)\\times ...\\times T_d(n)\\big)| \\geq \\epsilon |T_1(n)\\times ...\\times T_d(n)|\\] for every $n\\in L$, then there exist strong subtrees $(S_1,...,S_d)$ of $(T_1,...,T_d)$ of height $k$ and with common level set such ...


    Manoj K. Chaudhary


    Full Text Available Khoshnevisan et al. (2007 proposed a general family of estimators for population mean using known value of some population parameters in simple random sampling. The objective of this paper is to propose a family of combined-type estimators in stratified random sampling adapting the family of estimators proposed by Khoshnevisan et al. (2007 under non-response. The properties of proposed family have been discussed. We have also obtained the expressions for optimum sample sizes of the strata in respect to cost of the survey. Results are also supported by numerical analysis.

  4. Stratified spaces constitute a Fra\\"iss\\'e category

    Mijares, José Gregorio


    We prove that stratified spaces and stratified pseudomanifolds satisfy categorical Fra\\"{\\i}ss\\'e properties. This result was presented for the First Meeting of Logic and Algebra in Bogot\\'a, on Sept. 2010. This article has been submitted to the Revista Colombiana de Matem\\'aticas.

  5. Sensor Placement for Modal Parameter Subset Estimation

    Ulriksen, Martin Dalgaard; Bernal, Dionisio; Damkilde, Lars


    The present paper proposes an approach for deciding on sensor placements in the context of modal parameter estimation from vibration measurements. The approach is based on placing sensors, of which the amount is determined a priori, such that the minimum Fisher information that the frequency...... responses carry on the selected modal parameter subset is, in some sense, maximized. The approach is validated in the context of a simple 10-DOF mass-spring-damper system by computing the variance of a set of identified modal parameters in a Monte Carlo setting for a set of sensor configurations, whose......). It is shown that the widely used Effective Independence (EI) method, which uses the modal amplitudes as surrogates for the parameters of interest, provides sensor configurations yielding theoretical lower bound variances whose maxima are up to 30 % larger than those obtained by use of the max-min approach....

  6. Gas slug ascent through rheologically stratified conduits

    Capponi, Antonio; James, Mike R.; Lane, Steve J.


    Textural and petrological evidence has indicated the presence of viscous, degassed magma layers at the top of the conduit at Stromboli. This layer acts as a plug through which gas slugs burst and it is thought to have a role in controlling the eruptive dynamics. Here, we present the results of laboratory experiments which detail the range of slug flow configurations that can develop in a rheologically stratified conduit. A gas slug can burst (1) after being fully accommodated within the plug volume, (2) whilst its base is still in the underlying low-viscosity liquid or (3) within a low-viscosity layer dynamically emplaced above the plug during the slug ascent. We illustrate the relevance of the same flow configurations at volcanic-scale through a new experimentally-validated 1D model and 3D computational fluid dynamic simulations. Applied to Stromboli, our results show that gas volume, plug thickness, plug viscosity and conduit radius control the transition between each configuration; in contrast, the configuration distribution seems insensitive to the viscosity of magma beneath the plug, which acts mainly to deliver the slug into the plug. Each identified flow configuration encompasses a variety of processes including dynamic narrowing and widening of the conduit, generation of instabilities along the falling liquid film, transient blockages of the slug path and slug break-up. All these complexities, in turn, lead to variations in the slug overpressure, mirrored by changes in infrasonic signatures which are also associated to different eruptive styles. Acoustic amplitudes are strongly dependent on the flow configuration in which the slugs burst, with both acoustic peak amplitudes and waveform shapes reflecting different burst dynamics. When compared to infrasonic signals from Stromboli, the similarity between real signals and laboratory waveforms suggests that the burst of a slug through a plug may represent a viable first-order mechanism for the generation of

  7. Methane metabolism in a stratified boreal lake

    Nykänen, Hannu; Peura, Sari; Kankaala, Paula; Jones, Roger


    Stratified lakes, typical of the boreal zone, are naturally anoxic from their bottoms. In these lakes methanogenesis can account for up to half of organic matter degradation. However, a major part of the methane (CH4) is oxidized in the water column before reaching the atmosphere. Since methanotrophs use CH4 as their sole carbon and energy source, much CH4-derived carbon is incorporated into their biomass. Microbially produced CH4 has strongly negative δ13C compared to other carbon forms in ecosystems, making it possible to follow its route in food webs. However, only a few studies have estimated the amount of this microbial biomass or its carbon stable isotopic composition due to difficulties in separating it from other biomass or from other carbon forms in the water column. We estimated methanotrophic biomass from measured CH4 oxidation, and δ13C of the biomass from measured δ13C values of CH4, DIC, POM and DOC. An estimate of the fraction of methanotrophs in total microbial biomass is derived from bacterial community composition measurements. The study was made in, Alinen Mustajärvi, a small (area 0.75 ha, maximum depth 6.5 m, mean depth 4.2 m,), oligotrophic, mesohumic headwater lake located in boreal coniferous forest in southern Finland. CH4 and DIC concentrations and their δ13C were measured over the deepest point of the lake at 1 m intervals. 13C of DOM and POM were analyzed from composite samples from epi-, meta-, and hypolimnion. Evasion of CH4 and carbon dioxide from the lake surface to the atmosphere was estimated with boundary layer diffusion equations. CH4oxidation was estimated by comparing differences between observed concentrations and CH4potentially transported by turbulent diffusion between different vertical layers in the lake and also by actual methanotrophy measurements and from vertical differences in δ13C-CH4. The estimate of CH4 production was based on the sum of oxidized and released CH4. Molecular microbiology methods were used to

  8. Dissimilarity-Based Sparse Subset Selection.

    Elhamifar, Ehsan; Sapiro, Guillermo; Sastry, S Shankar


    Finding an informative subset of a large collection of data points or models is at the center of many problems in computer vision, recommender systems, bio/health informatics as well as image and natural language processing. Given pairwise dissimilarities between the elements of a 'source set' and a 'target set,' we consider the problem of finding a subset of the source set, called representatives or exemplars, that can efficiently describe the target set. We formulate the problem as a row-sparsity regularized trace minimization problem. Since the proposed formulation is, in general, NP-hard, we consider a convex relaxation. The solution of our optimization finds representatives and the assignment of each element of the target set to each representative, hence, obtaining a clustering. We analyze the solution of our proposed optimization as a function of the regularization parameter. We show that when the two sets jointly partition into multiple groups, our algorithm finds representatives from all groups and reveals clustering of the sets. In addition, we show that the proposed framework can effectively deal with outliers. Our algorithm works with arbitrary dissimilarities, which can be asymmetric or violate the triangle inequality. To efficiently implement our algorithm, we consider an Alternating Direction Method of Multipliers (ADMM) framework, which results in quadratic complexity in the problem size. We show that the ADMM implementation allows to parallelize the algorithm, hence further reducing the computational time. Finally, by experiments on real-world datasets, we show that our proposed algorithm improves the state of the art on the two problems of scene categorization using representative images and time-series modeling and segmentation using representative models.

  9. Lymphocytes subsets in children with febrile convulsions.

    Tuncer, Oğuz; Karaman, Sait; Caksen, Hüseyin; Oner, Ahmet Faik; Odabas, Dursun; Yilmaz, Cahide; Atas, Bülent


    In this study, lymphocytes subsets including blood CD3, CD4, CD8, CD16, CD19, and CD56 values were analyzed in children with febrile convulsion (FC) to determine whether there was the association of lymphocytes subsets in the pathogenesis of FC. The study includes 48 children with FC, and 55 healthy age matched control subjects, followed in Yüzüncü Yil University, Faculty of Medicine, Department of Pediatrics between October 2003 and June 2004. Blood CD3, CD4, CD8, CD16, CD19, and CD56 values were examined in the study and control groups. The analyses were performed in the Hematology Laboratory, Yüzüncü Yil University Faculty of Medicine, with flow cytometer device (Coulter Epics XL2, Flow Cytometer). A total of 48 children [17 girls (35.5%) and 31 boys (64.5%)], aged 6 months to 60 months (mean 22.20 +/- 13.75 months) with FC and 55 healthy children [28 girls (51%) and 27 boys (49%)], aged 6 months to 60 months (mean 28.87 +/- 17.04 months) were included in the study. When compared with the control group, the study found significantly decreased blood CD3 and CD4 values in the study group (p .05). When comparing the children with and without positive family history for FC, the study did not find any difference for all CD values between the groups (p >.05). Similarly, there was not significant difference in CD values between the children with simple and complex FC (p >.05). The findings suggested that decreased blood CD3 and CD4 values might be responsible for the infections connected with FC or that they might be related to the pathogenesis of FC in some children.

  10. The Universal Aspect Ratio of Vortices in Rotating Stratifi?ed Flows: Experiments and Observations

    Aubert, Oriane; Gal, Patrice Le; Marcus, Philip S


    We validate a new law for the aspect ratio $\\alpha = H/L$ of vortices in a rotating, stratified flow, where $H$ and $L$ are the vertical half-height and horizontal length scale of the vortices. The aspect ratio depends not only on the Coriolis parameter f and buoyancy (or Brunt-Vaisala) frequency $\\bar{N}$ of the background flow, but also on the buoyancy frequency $N_c$ within the vortex and on the Rossby number $Ro$ of the vortex such that $\\alpha = f \\sqrt{[Ro (1 + Ro)/(N_c^2- \\bar{N}^2)]}$. This law for $\\alpha$ is obeyed precisely by the exact equilibrium solution of the inviscid Boussinesq equations that we show to be a useful model of our laboratory vortices. The law is valid for both cyclones and anticyclones. Our anticyclones are generated by injecting fluid into a rotating tank filled with linearly-stratified salt water. The vortices are far from the top and bottom boundaries of the tank, so there is no Ekman circulation. In one set of experiments, the vortices viscously decay, but as they do, they c...

  11. Diagnostic accuracy of the STRATIFY clinical prediction rule for falls: A systematic review and meta-analysis

    Billington, Jennifer


    AbstractBackgroundThe STRATIFY score is a clinical prediction rule (CPR) derived to assist clinicians to identify patients at risk of falling. The purpose of this systematic review and meta-analysis is to determine the overall diagnostic accuracy of the STRATIFY rule across a variety of clinical settings.MethodsA literature search was performed to identify all studies that validated the STRATIFY rule. The methodological quality of the studies was assessed using the Quality Assessment of Diagnostic Accuracy Studies tool. A STRATIFY score of ≥2 points was used to identify individuals at higher risk of falling. All included studies were combined using a bivariate random effects model to generate pooled sensitivity and specificity of STRATIFY at ≥2 points. Heterogeneity was assessed using the variance of logit transformed sensitivity and specificity.ResultsSeventeen studies were included in our meta-analysis, incorporating 11,378 patients. At a score ≥2 points, the STRATIFY rule is more useful at ruling out falls in those classified as low risk, with a greater pooled sensitivity estimate (0.67, 95% CI 0.52–0.80) than specificity (0.57, 95% CI 0.45 – 0.69). The sensitivity analysis which examined the performance of the rule in different settings and subgroups also showed broadly comparable results, indicating that the STRATIFY rule performs in a similar manner across a variety of different ‘at risk’ patient groups in different clinical settings.ConclusionThis systematic review shows that the diagnostic accuracy of the STRATIFY rule is limited and should not be used in isolation for identifying individuals at high risk of falls in clinical practice.

  12. Epidemic characteristics of gout and primary hyperuricemia in Shandong coastal area: A randomized stratified cluster sampling survey%沿海居民痛风及高尿酸血症流行特点的随机、分层、整群抽样调查

    苗志敏; 赵世华; 王颜刚; 李长贵; 王忠超; 陈颖; 陈新焰; 阎胜利


    old in Shandong coastal area.DESIGN: A randomized, stratified cluster sampling survey.SETTING: D

  13. Recommending Cut Scores with a Subset of Items: An Empirical Illustration

    Chad W. Buckendahl


    Full Text Available Many testing programs face the practical challenge of having limited resources to conduct comprehensive standard setting studies. Some researchers have suggested that replicating a group's recommended cut score on a full-length test may be possible by using a subset of the items. However, these studies were based on simulated data. This study describes a standard setting application using two independent panels providing judgments on a 300-item licensure test. Specifically, one panel provided judgments on all 300 items; whereas the second panel made judgments on a randomly-selected subset of 150 items. Both panels also participated in an alternate standard setting method to evaluate panel comparability. Results suggest caution for practitioners considering using subsets of items for standard setting studies.

  14. Stratified Sampling of Neighborhood Sections for Population Estimation: A Case Study of Bo City, Sierra Leone.

    Hillson, Roger; Alejandre, Joel D; Jacobsen, Kathryn H; Ansumana, Rashid; Bockarie, Alfred S; Bangura, Umaru; Lamin, Joseph M; Stenger, David A


    There is a need for better estimators of population size in places that have undergone rapid growth and where collection of census data is difficult. We explored simulated estimates of urban population based on survey data from Bo, Sierra Leone, using two approaches: (1) stratified sampling from across 20 neighborhoods and (2) stratified single-stage cluster sampling of only four randomly-sampled neighborhoods. The stratification variables evaluated were (a) occupants per individual residence, (b) occupants per neighborhood, and (c) residential structures per neighborhood. For method (1), stratification variable (a) yielded the most accurate re-estimate of the current total population. Stratification variable (c), which can be estimated from aerial photography and zoning type verification, and variable (b), which could be ascertained by surveying a limited number of households, increased the accuracy of method (2). Small household-level surveys with appropriate sampling methods can yield reasonably accurate estimations of urban populations.

  15. Inferences from Genomic Models in Stratified Populations

    Janss, Luc; de los Campos, Gustavo; Sheehan, Nuala


    Unaccounted population stratification can lead to spurious associations in genome-wide association studies (GWAS) and in this context several methods have been proposed to deal with this problem. An alternative line of research uses whole-genome random regression (WGRR) models that fit all markers...... are unsatisfactory. Here we address this problem and describe a reparameterization of a WGRR model, based on an eigenvalue decomposition, for simultaneous inference of parameters and unobserved population structure. This allows estimation of genomic parameters with and without inclusion of marker......-derived eigenvectors that account for stratification. The method is illustrated with grain yield in wheat typed for 1279 genetic markers, and with height, HDL cholesterol and systolic blood pressure from the British 1958 cohort study typed for 1 million SNP genotypes. Both sets of data show signs of population...

  16. Large convexly independent subsets of Minkowski sums

    Swanepoel, Konrad J


    Let $E_d(n)$ be the maximum number of pairs that can be selected from a set of $n$ points in $R^d$ such that the midpoints of these pairs are convexly independent. We show that $E_2(n)\\geq \\Omega(n\\sqrt{\\log n})$, which answers a question of Eisenbrand, Pach, Rothvo\\ss, and Sopher (2008) on large convexly independent subsets in Minkowski sums of finite planar sets, as well as a question of Halman, Onn, and Rothblum (2007). We also show that $\\lfloor\\frac{1}{3}n^2\\rfloor\\leq E_3(n)\\leq \\frac{3}{8}n^2+O(n^{3/2})$. Let $W_d(n)$ be the maximum number of pairwise nonparallel unit distance pairs in a set of $n$ points in some $d$-dimensional strictly convex normed space. We show that $W_2(n)=\\Theta(E_2(n))$ and for $d\\geq 3$ that $W_d(n)\\sim\\frac12\\left(1-\\frac{1}{a(d)}\\right)n^2$, where $a(d)\\in N$ is related to strictly antipodal families. In fact we show that the same asymptotics hold without the requirement that the unit distance pairs form pairwise nonparallel segments, and also if diameter pairs are considere...

  17. Generalized Subset Designs in Analytical Chemistry.

    Surowiec, Izabella; Vikström, Ludvig; Hector, Gustaf; Johansson, Erik; Vikström, Conny; Trygg, Johan


    Design of experiments (DOE) is an established methodology in research, development, manufacturing, and production for screening, optimization, and robustness testing. Two-level fractional factorial designs remain the preferred approach due to high information content while keeping the number of experiments low. These types of designs, however, have never been extended to a generalized multilevel reduced design type that would be capable to include both qualitative and quantitative factors. In this Article we describe a novel generalized fractional factorial design. In addition, it also provides complementary and balanced subdesigns analogous to a fold-over in two-level reduced factorial designs. We demonstrate how this design type can be applied with good results in three different applications in analytical chemistry including (a) multivariate calibration using microwave resonance spectroscopy for the determination of water in tablets, (b) stability study in drug product development, and (c) representative sample selection in clinical studies. This demonstrates the potential of generalized fractional factorial designs to be applied in many other areas of analytical chemistry where representative, balanced, and complementary subsets are required, especially when a combination of quantitative and qualitative factors at multiple levels exists.

  18. Identification of polarized macrophage subsets in zebrafish.

    Nguyen-Chi, Mai; Laplace-Builhe, Béryl; Travnickova, Jana; Luz-Crawford, Patricia; Tejedor, Gautier; Phan, Quang Tien; Duroux-Richard, Isabelle; Levraud, Jean-Pierre; Kissa, Karima; Lutfalla, Georges; Jorgensen, Christian; Djouad, Farida


    While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tumour necrosis factor alpha (tnfa), a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa:eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and Escherichia coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on Fluorescence Activated Cell Sorting (FACS)-sorted tnfa(+) and tnfa(-) macrophages showed that they, respectively, expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa(+) macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.

  19. Tangling clustering instability for small particles in temperature stratified turbulence

    Elperin, Tov; Liberman, Michael; Rogachevskii, Igor


    We study particle clustering in a temperature stratified turbulence with small finite correlation time. It is shown that the temperature stratified turbulence strongly increases the degree of compressibility of particle velocity field. This results in the strong decrease of the threshold for the excitation of the tangling clustering instability even for small particles. The tangling clustering instability in the temperature stratified turbulence is essentially different from the inertial clustering instability that occurs in non-stratified isotropic and homogeneous turbulence. While the inertial clustering instability is caused by the centrifugal effect of the turbulent eddies, the mechanism of the tangling clustering instability is related to the temperature fluctuations generated by the tangling of the mean temperature gradient by the velocity fluctuations. Temperature fluctuations produce pressure fluctuations and cause particle clustering in regions with increased pressure fluctuations. It is shown that t...

  20. Effects of rotation on turbulent buoyant plumes in stratified environments

    Fabregat Tomàs, Alexandre; Poje, Andrew C; Özgökmen, Tamay M; Dewar, William K


    We numerically investigate the effects of rotation on the turbulent dynamics of thermally driven buoyant plumes in stratified environments at the large Rossby numbers characteristic of deep oceanic releases...

  1. Numerical Study on Saltwater Instrusion in a Heterogeneous Stratified Aquifer


    In a costal aquifer, saltwater intrusion is frequently observed due to an excess exploitation. There are many researches focused on the saltwater intrusion. However, there are few researches, which take into consideration the mixing processes in a stratified heterogeneous aquifer. In the present study, a laboratory experiment and numerical simulation are made in order to understand the phenomena in a stratified heterogeneous aquifer. The result of the numerical analysis agrees well with the m...

  2. Arithmetic progressions in Salem-type subsets of the integers

    Potgieter, Paul


    Given a subset of the integers of zero density, we define the weaker notion of fractional density of such a set. It is shown how this notion corresponds to that of the Hausdorff dimension of a compact subset of the reals. We then show that a version of a theorem of {\\L}aba and Pramanik on 3-term arithmetic progressions in subsets of the unit interval also holds for subsets of the integers with fractional density and satisfying certain Fourier-decay conditions.

  3. T-lymphocyte subsets in recurrent aphthous ulceration

    Pedersen, A; Klausen, B; Hougen, H P;


    Peripheral T-lymphocyte subsets: T-helper (OKT4) and T-suppressor (OKT8) cells were studied quantitatively in 20 patients with recurrent aphthous ulceration (RAU) in ulcerative, as well as inactive, stages of the disease. The figures were compared with T-lymphocyte subsets from matched control do...

  4. Stability of stratified two-phase flows in horizontal channels

    Barmak, Ilya; Ullmann, Amos; Brauner, Neima; Vitoshkin, Helen


    Linear stability of stratified two-phase flows in horizontal channels to arbitrary wavenumber disturbances is studied. The problem is reduced to Orr-Sommerfeld equations for the stream function disturbances, defined in each sublayer and coupled via boundary conditions that account also for possible interface deformation and capillary forces. Applying the Chebyshev collocation method, the equations and interface boundary conditions are reduced to the generalized eigenvalue problems solved by standard means of numerical linear algebra for the entire spectrum of eigenvalues and the associated eigenvectors. Some additional conclusions concerning the instability nature are derived from the most unstable perturbation patterns. The results are summarized in the form of stability maps showing the operational conditions at which a stratified-smooth flow pattern is stable. It is found that for gas-liquid and liquid-liquid systems the stratified flow with smooth interface is stable only in confined zone of relatively lo...

  5. Background Oriented Schlieren in a Density Stratified Fluid

    Verso, Lilly


    Non-intrusive quantitative fluid density measurements methods are essential in stratified flow experiments. Digital imaging leads to synthetic Schlieren methods in which the variations of the index of refraction are reconstructed computationally. In this study, an important extension to one of these methods, called Background Oriented Schlieren (BOS), is proposed. The extension enables an accurate reconstruction of the density field in stratified liquid experiments. Typically, the experiments are performed by the light source, background pattern, and the camera positioned on the opposite sides of a transparent vessel. The multi-media imaging through air-glass-water-glass-air leads to an additional aberration that destroys the reconstruction. A two-step calibration and image remapping transform are the key components that correct the images through the stratified media and provide non-intrusive full-field density measurements of transparent liquids.

  6. Background oriented schlieren in a density stratified fluid

    Verso, Lilly; Liberzon, Alex


    Non-intrusive quantitative fluid density measurement methods are essential in the stratified flow experiments. Digital imaging leads to synthetic schlieren methods in which the variations of the index of refraction are reconstructed computationally. In this study, an extension to one of these methods, called background oriented schlieren, is proposed. The extension enables an accurate reconstruction of the density field in stratified liquid experiments. Typically, the experiments are performed by the light source, background pattern, and the camera positioned on the opposite sides of a transparent vessel. The multimedia imaging through air-glass-water-glass-air leads to an additional aberration that destroys the reconstruction. A two-step calibration and image remapping transform are the key components that correct the images through the stratified media and provide a non-intrusive full-field density measurements of transparent liquids.

  7. SINDA/FLUINT Stratified Tank Modeling for Cryrogenic Propellant Tanks

    Sakowski, Barbara


    A general purpose SINDA/FLUINT (S/F) stratified tank model was created to simulate self-pressurization and axial jet TVS; Stratified layers in the vapor and liquid are modeled using S/F lumps.; The stratified tank model was constructed to permit incorporating the following additional features:, Multiple or singular lumps in the liquid and vapor regions of the tank, Real gases (also mixtures) and compressible liquids, Venting, pressurizing, and draining, Condensation and evaporation/boiling, Wall heat transfer, Elliptical, cylindrical, and spherical tank geometries; Extensive user logic is used to allow detailed tailoring - Don't have to rebuilt everything from scratch!!; Most code input for a specific case is done through the Registers Data Block:, Lump volumes are determined through user input:; Geometric tank dimensions (height, width, etc); Liquid level could be input as either a volume percentage of fill level or actual liquid level height

  8. Fuel Burning Rate Model for Stratified Charge Engine

    SONG Jin'ou; JIANG Zejun; YAO Chunde; WANG Hongfu


    A zero-dimensional single-zone double-curve model is presented to predict fuel burning rate in stratified charge engines, and it is integrated with GT-Power to predict the overall performance of the stratified charge engines.The model consists of two exponential functions for calculating the fuel burning rate in different charge zones.The model factors are determined by a non-linear curve fitting technique, based on the experimental data obtained from 30 cases in middle and low loads.The results show good agreement between the measured and calculated cylinder pressures,and the deviation between calculated and measured cylinder pressures is less than 5%.The zerodimensional single-zone double-curve model is successful in the combustion modeling for stratified charge engines.

  9. Numerical Simulation on Stratified Flow over an Isolated Mountain Ridge

    LI Ling; Shigeo Kimura


    The characteristics of stratified flow over an isolated mountain ridge have been investigated numerically. The two-dimensional model equations, based on the time-dependent Reynolds averaged NavierStokes equations, are solved numerically using an implicit time integration in a fitted body grid arrangement to simulate stratified flow over an isolated ideally bell-shaped mountain. The simulation results are in good agreement with the existing corresponding analytical and approximate solutions. It is shown that for atmospheric conditions where non-hydrostatic effects become dominant, the model is able to reproduce typical flow features. The dispersion characteristics of gaseous pollutants in the stratified flow have also been studied. The dispersion patterns for two typical atmospheric conditions are compared. The results show that the presence of a gravity wave causes vertical stratification of the pollutant concentration and affects the diffusive characteristics of the pollutants.

  10. Stability of stratified two-phase flows in inclined channels

    Barmak, Ilya; Ullmann, Amos; Brauner, Neima


    Linear stability of stratified gas-liquid and liquid-liquid plane-parallel flows in inclined channels is studied with respect to all wavenumber perturbations. The main objective is to predict parameter regions in which stable stratified configuration in inclined channels exists. Up to three distinct base states with different holdups exist in inclined flows, so that the stability analysis has to be carried out for each branch separately. Special attention is paid to the multiple solution regions to reveal the feasibility of non-unique stable stratified configurations in inclined channels. The stability boundaries of each branch of steady state solutions are presented on the flow pattern map and are accompanied by critical wavenumbers and spatial profiles of the most unstable perturbations. Instabilities of different nature are visualized by streamlines of the neutrally stable perturbed flows, consisting of the critical perturbation superimposed on the base flow. The present analysis confirms the existence of ...

  11. Basonuclin regulates a subset of ribosomal RNA genes in HaCaT cells.

    Shengliang Zhang

    Full Text Available Basonuclin (Bnc1, a cell-type-specific ribosomal RNA (rRNA gene regulator, is expressed mainly in keratinocytes of stratified epithelium and gametogenic cells of testis and ovary. Previously, basonuclin was shown in vitro to interact with rRNA gene (rDNA promoter at three highly conserved sites. Basonuclin's high affinity binding site overlaps with the binding site of a dedicated and ubiquitous Pol I transcription regulator, UBF, suggesting that their binding might interfere with each other if they bind to the same promoter. Knocking-down basonuclin in mouse oocytes eliminated approximately one quarter of RNA polymerase I (Pol I transcription foci, without affecting the BrU incorporation of the remaining ones, suggesting that basonuclin might regulate a subset of rDNA. Here we show, via chromatin immunoprecipitation (ChIP, that basonuclin is associated with rDNA promoters in HaCaT cells, a spontaneously established human keratinocyte line. Immunoprecipitation data suggest that basonuclin is in a complex that also contains the subunits of Pol I (RPA194, RPA116, but not UBF. Knocking-down basonuclin in HaCaT cells partially impairs the association of RPA194 to rDNA promoter, but not that of UBF. Basonuclin-deficiency also reduces the amount of 47S pre-rRNA, but this effect can be seen only after cell-proliferation related rRNA synthesis has subsided at a higher cell density. DNA sequence of basonuclin-bound rDNA promoters shows single nucleotide polymorphisms (SNPs that differ from those associated with UBF-bound promoters, suggesting that basonuclin and UBF interact with different subsets of promoters. In conclusion, our results demonstrate basonuclin's functional association with rDNA promoters and its interaction with Pol I in vivo. Our data also suggest that basonuclin-Pol I complex transcribes a subset of rDNA.

  12. Sample Subset Optimization Techniques for Imbalanced and Ensemble Learning Problems in Bioinformatics Applications.

    Yang, Pengyi; Yoo, Paul D; Fernando, Juanita; Zhou, Bing B; Zhang, Zili; Zomaya, Albert Y


    Data sampling is a widely used technique in a broad range of machine learning problems. Traditional sampling approaches generally rely on random resampling from a given dataset. However, these approaches do not take into consideration additional information, such as sample quality and usefulness. We recently proposed a data sampling technique, called sample subset optimization (SSO). The SSO technique relies on a cross-validation procedure for identifying and selecting the most useful samples as subsets. In this paper, we describe the application of SSO techniques to imbalanced and ensemble learning problems, respectively. For imbalanced learning, the SSO technique is employed as an under-sampling technique for identifying a subset of highly discriminative samples in the majority class. In ensemble learning, the SSO technique is utilized as a generic ensemble technique where multiple optimized subsets of samples from each class are selected for building an ensemble classifier. We demonstrate the utilities and advantages of the proposed techniques on a variety of bioinformatics applications where class imbalance, small sample size, and noisy data are prevalent.

  13. Linear Inviscid Damping for Couette Flow in Stratified Fluid

    Yang, Jincheng


    We study the inviscid damping of Coutte flow with an exponentially stratified density. The optimal decay rates of the velocity field and density are obtained for general perturbations with minimal regularity. For Boussinesq approximation model, the decay rates we get are consistent with the previous results in the literature. We also study the decay rates for the full equations of stratified fluids, which were not studied before. For both models, the decay rates depend on the Richardson number in a very similar way. Besides, we also study the inviscid damping of perturbations due to the exponential stratification when there is no shear.

  14. Bases of Schur algebras associated to cellularly stratified diagram algebras

    Bowman, C


    We examine homomorphisms between induced modules for a certain class of cellularly stratified diagram algebras, including the BMW algebra, Temperley-Lieb algebra, Brauer algebra, and (quantum) walled Brauer algebra. We define the `permutation' modules for these algebras, these are one-sided ideals which allow us to study the diagrammatic Schur algebras of Hartmann, Henke, Koenig and Paget. We construct bases of these Schur algebras in terms of modified tableaux. On the way we prove that the (quantum) walled Brauer algebra and the Temperley-Lieb algebra are both cellularly stratified and therefore have well-defined Specht filtrations.


    Binayak S. Choudhury


    In the present paper we introduce a random iteration scheme for three random operators defined on a closed and convex subset of a uniformly convex Banach space and prove its convergence to a common fixed point of three random operators. The result is also an extension of a known theorem in the corresponding non-random case.

  16. A New Stratified Sampling Procedure which Decreases Error Estimation of Varroa Mite Number on Sticky Boards.

    Kretzschmar, A; Durand, E; Maisonnasse, A; Vallon, J; Le Conte, Y


    A new procedure of stratified sampling is proposed in order to establish an accurate estimation of Varroa destructor populations on sticky bottom boards of the hive. It is based on the spatial sampling theory that recommends using regular grid stratification in the case of spatially structured process. The distribution of varroa mites on sticky board being observed as spatially structured, we designed a sampling scheme based on a regular grid with circles centered on each grid element. This new procedure is then compared with a former method using partially random sampling. Relative error improvements are exposed on the basis of a large sample of simulated sticky boards (n=20,000) which provides a complete range of spatial structures, from a random structure to a highly frame driven structure. The improvement of varroa mite number estimation is then measured by the percentage of counts with an error greater than a given level.

  17. Random forest for gene selection and microarray data classification.

    Moorthy, Kohbalan; Mohamad, Mohd Saberi


    A random forest method has been selected to perform both gene selection and classification of the microarray data. In this embedded method, the selection of smallest possible sets of genes with lowest error rates is the key factor in achieving highest classification accuracy. Hence, improved gene selection method using random forest has been proposed to obtain the smallest subset of genes as well as biggest subset of genes prior to classification. The option for biggest subset selection is done to assist researchers who intend to use the informative genes for further research. Enhanced random forest gene selection has performed better in terms of selecting the smallest subset as well as biggest subset of informative genes with lowest out of bag error rates through gene selection. Furthermore, the classification performed on the selected subset of genes using random forest has lead to lower prediction error rates compared to existing method and other similar available methods.

  18. Analysis of photonic band-gap structures in stratified medium

    Tong, Ming-Sze; Yinchao, Chen; Lu, Yilong;


    Purpose - To demonstrate the flexibility and advantages of a non-uniform pseudo-spectral time domain (nu-PSTD) method through studies of the wave propagation characteristics on photonic band-gap (PBG) structures in stratified medium Design/methodology/approach - A nu-PSTD method is proposed...

  19. Plane Stratified Flow in a Room Ventilated by Displacement Ventilation

    Nielsen, Peter Vilhelm; Nickel, J.; Baron, D. J. G.


    The air movement in the occupied zone of a room ventilated by displacement ventilation exists as a stratified flow along the floor. This flow can be radial or plane according to the number of wall-mounted diffusers and the room geometry. The paper addresses the situations where plane flow...

  20. Bacterial production, protozoan grazing and mineralization in stratified lake Vechten.

    Bloem, J.


    The role of heterotrophic nanoflagellates (HNAN, size 2-20 μm) in grazing on bacteria and mineralization of organic matter in stratified Lake Vechten was studied.Quantitative effects of manipulation and fixation on HNAN were checked. Considerable losses were caused by centrifugation, even at low spe

  1. Population dynamics of sinking phytoplankton in stratified waters

    Huisman, J.; Sommeijer, B.P.


    We analyze the predictions of a reaction-advection-diffusion model to pinpoint the necessary conditions for bloom development of sinking phytoplanktonspecies in stratified waters. This reveals that there are two parameter windows that can sustain sinking phytoplankton, a turbulence window and atherm

  2. Gravity-induced stresses in stratified rock masses

    Amadei, B.; Swolfs, H.S.; Savage, W.Z.


    This paper presents closed-form solutions for the stress field induced by gravity in anisotropic and stratified rock masses. These rocks are assumed to be laterally restrained. The rock mass consists of finite mechanical units, each unit being modeled as a homogeneous, transversely isotropic or isotropic linearly elastic material. The following results are found. The nature of the gravity induced stress field in a stratified rock mass depends on the elastic properties of each rock unit and how these properties vary with depth. It is thermodynamically admissible for the induced horizontal stress component in a given stratified rock mass to exceed the vertical stress component in certain units and to be smaller in other units; this is not possible for the classical unstratified isotropic solution. Examples are presented to explore the nature of the gravity induced stress field in stratified rock masses. It is found that a decrease in rock mass anisotropy and a stiffening of rock masses with depth can generate stress distributions comparable to empirical hyperbolic distributions previously proposed in the literature. ?? 1988 Springer-Verlag.

  3. Dispersion of (light) inertial particles in stratified turbulence

    van Aartrijk, M.; Clercx, H.J.H.; Armenio, Vincenzo; Geurts, Bernardus J.; Fröhlich, Jochen


    We present a brief overview of a numerical study of the dispersion of particles in stably stratified turbulence. Three types of particles arc examined: fluid particles, light inertial particles ($\\rho_p/\\rho_f = \\mathcal{O}(1)$) and heavy inertial particles ($\\rho_p/\\rho_f \\gg 1$). Stratification

  4. The dynamics of small inertial particles in weakly stratified turbulence

    van Aartrijk, M.; Clercx, H.J.H.

    We present an overview of a numerical study on the small-scale dynamics and the large-scale dispersion of small inertial particles in stably stratified turbulence. Three types of particles are examined: fluid particles, light inertial particles (with particle-to-fluid density ratio 1Ͽp/Ͽf25) and

  5. Characterization of Inlet Diffuser Performance for Stratified Thermal Storage

    Cimbala, John M.; Bahnfleth, William; Song, Jing


    Storage of sensible heating or cooling capacity in stratified vessels has important applications in central heating and cooling plants, power production, and solar energy utilization, among others. In stratified thermal storage systems, diffusers at the top and bottom of a stratified tank introduce and withdraw fluid while maintaining a stable density gradient and causing as little mixing as possible. In chilled water storage applications, mixing during the formation of the thermocline near an inlet diffuser is the single greatest source of thermal losses. Most stratified chilled water storage tanks are cylindrical vessels with diffusers that are either circular disks that distribute flow radially outward or octagonal rings of perforated pipe that distribute flow both inward and outward radially. Both types produce gravity currents that are strongly influenced by the inlet Richardson number, but the significance of other parameters is not clear. The present investigation considers the dependence of the thermal performance of a perforated pipe diffuser on design parameters including inlet velocity, ambient and inlet fluid temperatures, and tank dimensions for a range of conditions representative of typical chilled water applications. Dimensional analysis is combined with a parametric study using results from computational fluid dynamics to obtain quantitative relationships between design parameters and expected thermal performance.

  6. Global and Partial Errors in Stratified and Clustering Sampling

    Giovanna Nicolini; Anna Lo Presti


    In this paper we split up the sampling error occurred in stratified and clustering sampling, called global error and measured by the variance of estimator, in many partial errors each one referred to a single stratum or cluster. In particular, we study, for clustering sampling, the empirical distribution of the homogeneity coefficient that is very important for settlement of partial errors.

  7. Codings of separable compact subsets of the first Baire class

    Dodos, Pandelis


    Let $X$ be a Polish space and $K$ a separable compact subset of the first Baire class on $X$. For every sequence $\\bs$ dense in $\\kk$, the descriptive set-theoretic properties of the set \\[ \\lbf=\\{L\\in[\

  8. Semilinear elliptic problems on unbounded subsets of the Heisenberg group

    K. Tintarev


    Full Text Available In this paper we discuss the applications of an abstract version of concentration compactness to minimax problems. In particular, we prove the existence of solutions to semilinear elliptic problems on unbounded subsets of the Heisenberg group.

  9. Reliability analysis and updating of deteriorating systems with subset simulation

    Schneider, Ronald; Thöns, Sebastian; Straub, Daniel


    Bayesian updating of the system deterioration model. The updated system reliability is then obtained through coupling the updated deterioration model with a probabilistic structural model. The underlying high-dimensional structural reliability problems are solved using subset simulation, which...

  10. Ineffective oesophageal motility: Manometric subsets exhibit different symptom profiles

    Horst Gunter Haack; Ross David Hansen; Allison Malcolm; John Edward Kellow


    AIM: To compare the demographic and clinical features of different manometric subsets of ineffective oesophageal motility (IOM; defined as ≥30% wet swallows with distal contractile amplitude <30 mmHg), and to determine whether the prevalence of gastro-oesophageal reflux differs between IOM subsets.METHODS: Clinical characteristics of manometric subsets were determined in 100 IOM patients (73 female, median age 58 years) and compared to those of 100 age-and gender-matched patient controls with oesophageal symptoms, but normal manometry. Supine oesophageal manometry was performed with an eight-channel DentSleeve water-perfused catheter, and an ambulatory pH study assessed gastrooesophageal reflux.RESULTS: Patients in the IOM subset featuring a majority of low-amplitude simultaneous contractions (LASC) experienced less heartburn (prevalence 26%), but more dysphagia (57%) than those in the IOM subset featuring low-amplitude propagated contractions (LAP; heartburn 70%, dysphagia 24%; both P≤0.01). LASC patients also experienced less heartburn and more dysphagia than patient controls (heartburn 68%, dysphagia 11%; both P<0.001). The prevalence of heartburn and dysphagia in IOM patients featuring a majority of non-transmitted sequences (NT) was 54% (P=0.04 vs LASC) and 36% (P<0.01 vs controls), respectively. No differences in age and gender distribution, chest pain prevalence, acid exposure time (AET) and symptom/reflux association existed between IOM subsets, or between subsets and controls.CONCLUSION: IOM patients with LASC exhibit a different symptom profile to those with LAP, but do not differ in gastro-oesophageal reflux prevalence. These findings raise the possibility of different pathophysiological mechanisms in IOM subsets, which warrants further investigation.

  11. Graph Polynomials: From Recursive Definitions To Subset Expansion Formulas

    Godlin, Benny; Makowsky, Johann A


    Many graph polynomials, such as the Tutte polynomial, the interlace polynomial and the matching polynomial, have both a recursive definition and a defining subset expansion formula. In this paper we present a general, logic-based framework which gives a precise meaning to recursive definitions of graph polynomials. We then prove that in this framework every recursive definition of a graph polynomial can be converted into a subset expansion formula.

  12. Domino: Extracting, Comparing, and Manipulating Subsets across Multiple Tabular Datasets

    Gratzl, Samuel; Gehlenborg, Nils; Lex, Alexander; Pfister, Hanspeter; Streit, Marc


    Answering questions about complex issues often requires analysts to take into account information contained in multiple interconnected datasets. A common strategy in analyzing and visualizing large and heterogeneous data is dividing it into meaningful subsets. Interesting subsets can then be selected and the associated data and the relationships between the subsets visualized. However, neither the extraction and manipulation nor the comparison of subsets is well supported by state-of-the-art techniques. In this paper we present Domino, a novel multiform visualization technique for effectively representing subsets and the relationships between them. By providing comprehensive tools to arrange, combine, and extract subsets, Domino allows users to create both common visualization techniques and advanced visualizations tailored to specific use cases. In addition to the novel technique, we present an implementation that enables analysts to manage the wide range of options that our approach offers. Innovative interactive features such as placeholders and live previews support rapid creation of complex analysis setups. We introduce the technique and the implementation using a simple example and demonstrate scalability and effectiveness in a use case from the field of cancer genomics. PMID:26356916

  13. Origin of CD8+ Effector and Memory T Cell Subsets

    Christian Stemberger; Michael Neuenhahn; Veit R.Buchholz; Dirk H.Busch


    It is well accepted that CD8+ T cells play a pivotal role in providing protection against infection with intracellular pathogens and some tumors. In many cases protective immunity is maintained for long periods of time (immunological memory). Over the past years, it has become evident that in order to fulfill these multiple tasks,distinct subsets of effector and memory T cells have to be generated. Until today, however, little is known about the underlying mechanisms of subset differentiation and the timing of lineage fate decisions. In this context, it is of special importance to determine at which level of clonal expansion functional and phenotypical heterogeneity is achieved. Different models for T cell subset diversification have been proposed; these differ mainly in the time point during priming and clonal expansion (prior, during, or beyond the first cell division) when differentiation programs are induced. Recently developed single-cell adoptive transfer technology has allowed us to demonstrate that individual precursor cell still bears the full plasticity to develop into a plethora different T cell subsets. This observation targets the shaping of T cell subset differentiation towards factors that are still operative beyond the first cell division. These findings have important implications for vaccine development, as the modulation of differentiation patterns towards distinct subsets could become a powerful strategy to enhance the efficacy and quality of vaccines.

  14. Indications for tonsillectomy stratified by the level of evidence

    Windfuhr, Jochen P.


    Background: One of the most significant clinical trials, demonstrating the efficacy of tonsillectomy (TE) for recurrent throat infection in severely affected children, was published in 1984. This systematic review was undertaken to compile various indications for TE as suggested in the literature after 1984 and to stratify the papers according to the current concept of evidence-based medicine. Material and methods: A systematic Medline research was performed using the key word of “tonsillectomy“ in combination with different filters such as “systematic reviews“, “meta-analysis“, “English“, “German“, and “from 1984/01/01 to 2015/05/31“. Further research was performed in the Cochrane Database of Systematic Reviews, National Guideline Clearinghouse, Guidelines International Network and BMJ Clinical Evidence using the same key word. Finally, data from the “Trip Database” were researched for “tonsillectomy” and “indication“ and “from: 1984 to: 2015“ in combination with either “systematic review“ or “meta-analysis“ or “metaanalysis”. Results: A total of 237 papers were retrieved but only 57 matched our inclusion criteria covering the following topics: peritonsillar abscess (3), guidelines (5), otitis media with effusion (5), psoriasis (3), PFAPA syndrome (6), evidence-based indications (5), renal diseases (7), sleep-related breathing disorders (11), and tonsillitis/pharyngitis (12), respectively. Conclusions: 1) The literature suggests, that TE is not indicated to treat otitis media with effusion. 2) It has been shown, that the PFAPA syndrome is self-limiting and responds well to steroid administration, at least in a considerable amount of children. The indication for TE therefore appears to be imbalanced but further research is required to clarify the value of surgery. 3) Abscesstonsillectomy as a routine is not justified and indicated only for cases not responding to other measures of treatment, evident complications

  15. Study of MRI in Stratified Viscous Plasma Configuration

    Carlevaro, Nakia; Renzi, Fabrizio


    We analyze the morphology of the Magneto-rotational Instability (MRI) for a stratified viscous plasma disk configuration in differential rotation, taking into account the so-called corotation theorem for the background profile. In order to select the intrinsic Alfv\\'enic nature of MRI, we deal with an incompressible plasma and we adopt a formulation of the perturbation analysis based on the use of the magnetic flux function as a dynamical variable. Our study outlines, as consequence of the corotation condition, a marked asymmetry of the MRI with respect to the equatorial plane, particularly evident in a complete damping of the instability over a positive critical height on the equatorial plane. We also emphasize how such a feature is already present (although less pronounced) even in the ideal case, restoring a dependence of the MRI on the stratified morphology of the gravitational field.

  16. FC-normal and extended stratified logic program

    许道云; 丁德成


    This paper investigates the consistency property of FC-normal logic program and presentsan equivalent deciding condition whether a logic program P is an FC-normal program. The decidingcondition describes the characterizations of FC-normal program. By the Petri-net presentation ofa logic program, the characterizations of stratification of FC-normal program are investigated. Thestratification of FC-normal program motivates us to introduce a new kind of stratification, extendedstratification, over logic program. It is shown that an extended (locally) stratified logic program isan FC-normal program. Thus, an extended (locally) stratified logic program has at least one stablemodel. Finally, we have presented algorithms about computation of consistency property and a fewequivalent deciding methods of the finite FC-normal program.

  17. Turbulent thermal diffusion in strongly stratified turbulence: theory and experiments

    Amir, G; Eidelman, A; Elperin, T; Kleeorin, N; Rogachevskii, I


    Turbulent thermal diffusion is a combined effect of the temperature stratified turbulence and inertia of small particles. It causes the appearance of a non-diffusive turbulent flux of particles in the direction of the turbulent heat flux. This non-diffusive turbulent flux of particles is proportional to the product of the mean particle number density and the effective velocity of inertial particles. The theory of this effect has been previously developed only for small temperature gradients and small Stokes numbers (Phys. Rev. Lett. {\\bf 76}, 224, 1996). In this study a generalized theory of turbulent thermal diffusion for arbitrary temperature gradients and Stokes numbers has been developed. The laboratory experiments in the oscillating grid turbulence and in the multi-fan produced turbulence have been performed to validate the theory of turbulent thermal diffusion in strongly stratified turbulent flows. It has been shown that the ratio of the effective velocity of inertial particles to the characteristic ve...

  18. Numerical Simulation of Wakes in a Weakly Stratified Fluid

    Rottman, James W; Innis, George E; O'Shea, Thomas T; Novikov, Evgeny


    This paper describes some preliminary numerical studies using large eddy simulation of full-scale submarine wakes. Submarine wakes are a combination of the wake generated by a smooth slender body and a number of superimposed vortex pairs generated by various control surfaces and other body appendages. For this preliminary study, we attempt to gain some insight into the behavior of full-scale submarine wakes by computing separately the evolution the self-propelled wake of a slender body and the motion of a single vortex pair in both a non-stratified and a stratified environment. An important aspect of the simulations is the use of an iterative procedure to relax the initial turbulence field so that turbulent production and dissipation are in balance.

  19. Axisymmetric modes in vertically stratified self-gravitating discs

    Mamatsashvili, George


    We perform linear analysis of axisymmetric vertical normal modes in stratified compressible self-gravitating polytropic discs in the shearing box approximation. We study specific dynamics for subadiabatic, adiabatic and superadiabatic vertical stratifications. In the absence of self-gravity, four well-known principal modes can be identified in a stratified disc: acoustic p-, surface gravity f-, buoyancy g- and inertial r-modes. After characterizing modes in the non-self-gravitating case, we include self-gravity and investigate how it modifies the properties of these modes. We find that self-gravity, to a certain degree, reduces their frequencies and changes the structure of the dispersion curves and eigenfunctions at radial wavelengths comparable to the disc height. Its influence on the basic branch of the r-mode, in the case of subadiabatic and adiabatic stratifications, and on the basic branch of the g-mode, in the case of superadiabatic stratification (which in addition exhibits convective instability), do...

  20. Elementary stratified flows with stability at low Richardson number

    Barros, Ricardo [Mathematics Applications Consortium for Science and Industry (MACSI), Department of Mathematics and Statistics, University of Limerick, Limerick (Ireland); Choi, Wooyoung [Department of Mathematical Sciences, New Jersey Institute of Technology, Newark, New Jersey 07102-1982 (United States)


    We revisit the stability analysis for three classical configurations of multiple fluid layers proposed by Goldstein [“On the stability of superposed streams of fluids of different densities,” Proc. R. Soc. A. 132, 524 (1931)], Taylor [“Effect of variation in density on the stability of superposed streams of fluid,” Proc. R. Soc. A 132, 499 (1931)], and Holmboe [“On the behaviour of symmetric waves in stratified shear layers,” Geophys. Publ. 24, 67 (1962)] as simple prototypes to understand stability characteristics of stratified shear flows with sharp density transitions. When such flows are confined in a finite domain, it is shown that a large shear across the layers that is often considered a source of instability plays a stabilizing role. Presented are simple analytical criteria for stability of these low Richardson number flows.

  1. Experiments on the dryout behavior of stratified debris beds

    Leininger, Simon; Kulenovic, Rudi; Laurien, Eckart [Stuttgart Univ. (Germany). Inst. of Nuclear Technology and Energy Systems (IKE)


    In case of a severe accident with loss of coolant and core meltdown a particle bed (debris) can be formed. The removal of decay heat from the debris bed is of prime importance for the bed's long-term coolability to guarantee the integrity of the RPV. In contrast to previous experiments, the focus is on stratified beds. The experiments have pointed out that the bed's coolability is significantly affected.

  2. Computation of mixing in large stably stratified enclosures

    Zhao, Haihua

    This dissertation presents a set of new numerical models for the mixing and heat transfer problems in large stably stratified enclosures. Basing on these models, a new computer code, BMIX++ (Berkeley mechanistic MIXing code in C++), was developed by Christensen (2001) and the author. Traditional lumped control volume methods and zone models cannot model the detailed information about the distributions of temperature, density, and pressure in enclosures and therefore can have significant errors. 2-D and 3-D CFD methods require very fine grid resolution to resolve thin substructures such as jets, wall boundaries, yet such fine grid resolution is difficult or impossible to provide due to computational expense. Peterson's scaling (1994) showed that stratified mixing processes in large stably stratified enclosures can be described using one-dimensional differential equations, with the vertical transport by free and wall jets modeled using standard integral techniques. This allows very large reductions in computational effort compared to three-dimensional numerical modeling of turbulent mixing in large enclosures. The BMIX++ code was developed to implement the above ideas. The code uses a Lagrangian approach to solve 1-D transient governing equations for the ambient fluid and uses analytical models or 1-D integral models to compute substructures. 1-D transient conduction model for the solid boundaries, pressure computation and opening models are also included to make the code more versatile. The BMIX++ code was implemented in C++ and the Object-Oriented-Programming (OOP) technique was intensively used. The BMIX++ code was successfully applied to different types of mixing problems such as stratification in a water tank due to a heater inside, water tank exchange flow experiment simulation, early stage building fire analysis, stratification produced by multiple plumes, and simulations for the UCB large enclosure experiments. Most of these simulations gave satisfying

  3. A statistical mechanics approach to mixing in stratified fluids

    Venaille, A.; Gostiaux, L.; Sommeria, J.


    Predicting how much mixing occurs when a given amount of energy is injected into a Boussinesq fluid is a longstanding problem in stratified turbulence. The huge number of degrees of freedom involved in those processes renders extremely difficult a deterministic approach to the problem. Here we present a statistical mechanics approach yielding prediction for a cumulative, global mixing efficiency as a function of a global Richardson number and the background buoyancy profile.

  4. Corticosteroids and pediatric septic shock outcomes: a risk stratified analysis.

    Sarah J Atkinson

    Full Text Available The potential benefits of corticosteroids for septic shock may depend on initial mortality risk.We determined associations between corticosteroids and outcomes in children with septic shock who were stratified by initial mortality risk.We conducted a retrospective analysis of an ongoing, multi-center pediatric septic shock clinical and biological database. Using a validated biomarker-based stratification tool (PERSEVERE, 496 subjects were stratified into three initial mortality risk strata (low, intermediate, and high. Subjects receiving corticosteroids during the initial 7 days of admission (n = 252 were compared to subjects who did not receive corticosteroids (n = 244. Logistic regression was used to model the effects of corticosteroids on 28-day mortality and complicated course, defined as death within 28 days or persistence of two or more organ failures at 7 days.Subjects who received corticosteroids had greater organ failure burden, higher illness severity, higher mortality, and a greater requirement for vasoactive medications, compared to subjects who did not receive corticosteroids. PERSEVERE-based mortality risk did not differ between the two groups. For the entire cohort, corticosteroids were associated with increased risk of mortality (OR 2.3, 95% CI 1.3-4.0, p = 0.004 and a complicated course (OR 1.7, 95% CI 1.1-2.5, p = 0.012. Within each PERSEVERE-based stratum, corticosteroid administration was not associated with improved outcomes. Similarly, corticosteroid administration was not associated with improved outcomes among patients with no comorbidities, nor in groups of patients stratified by PRISM.Risk stratified analysis failed to demonstrate any benefit from corticosteroids in this pediatric septic shock cohort.

  5. Dust particle charge distribution in a stratified glow discharge

    Sukhinin, Gennady I [Institute of Thermophysics, Siberian Branch, Russian Academy of Sciences, Lavrentyev Ave., 1, Novosibirsk 630090 (Russian Federation); Fedoseev, Alexander V [Institute of Thermophysics, Siberian Branch, Russian Academy of Sciences, Lavrentyev Ave., 1, Novosibirsk 630090 (Russian Federation); Ramazanov, Tlekkabul S [Institute of Experimental and Theoretical Physics, Al Farabi Kazakh National University, Tole Bi, 96a, Almaty 050012 (Kazakhstan); Dzhumagulova, Karlygash N [Institute of Experimental and Theoretical Physics, Al Farabi Kazakh National University, Tole Bi, 96a, Almaty 050012 (Kazakhstan); Amangaliyeva, Rauan Zh [Institute of Experimental and Theoretical Physics, Al Farabi Kazakh National University, Tole Bi, 96a, Almaty 050012 (Kazakhstan)


    The influence of a highly pronounced non-equilibrium characteristic of the electron energy distribution function in a stratified dc glow discharge on the process of dust particle charging in a complex plasma is taken into account for the first time. The calculated particle charge spatial distribution is essentially non-homogeneous and it can explain the vortex motion of particles at the periphery of a dusty cloud obtained in experiments.

  6. Stability of stratified two-phase flows in inclined channels

    Barmak, I.; Gelfgat, A. Yu.; Ullmann, A.; Brauner, N.


    Linear stability of the stratified gas-liquid and liquid-liquid plane-parallel flows in the inclined channels is studied with respect to all wavenumber perturbations. The main objective is to predict the parameter regions in which the stable stratified configuration in inclined channels exists. Up to three distinct base states with different holdups exist in the inclined flows, so that the stability analysis has to be carried out for each branch separately. Special attention is paid to the multiple solution regions to reveal the feasibility of the non-unique stable stratified configurations in inclined channels. The stability boundaries of each branch of the steady state solutions are presented on the flow pattern map and are accompanied by the critical wavenumbers and the spatial profiles of the most unstable perturbations. Instabilities of different nature are visualized by the streamlines of the neutrally stable perturbed flows, consisting of the critical perturbation superimposed on the base flow. The present analysis confirms the existence of two stable stratified flow configurations in a region of low flow rates in the countercurrent liquid-liquid flows. These configurations become unstable with respect to the shear mode of instability. It was revealed that in slightly upward inclined flows the lower and middle solutions for the holdup are stable in the part of the triple solution region, while the upper solution is always unstable. In the case of downward flows, in the triple solution region, none of the solutions are stable with respect to the short-wave perturbations. These flows are stable only in the single solution region at low flow rates of the heavy phase, and the long-wave perturbations are the most unstable ones.

  7. Thermal stratification built up in hot water tank with different inlet stratifiers

    Dragsted, Janne; Furbo, Simon; Dannemand, Mark


    H is a rigid plastic pipe with holes for each 30 cm. The holes are designed with flaps preventing counter flow into the pipe. The inlet stratifier from EyeCular Technologies ApS is made of a flexible polymer with openings all along the side and in the full length of the stratifier. The flexibility...... in order to elucidate how well thermal stratification is established in the tank with differently designed inlet stratifiers under different controlled laboratory conditions. The investigated inlet stratifiers are from Solvis GmbH & Co KG and EyeCular Technologies ApS. The inlet stratifier from Solvis Gmb...... of the stratifier prevents counterflow. The tests have shown that both types of inlet stratifiers had an ability to create stratification in the test tank under the different test conditions. The stratifier from EyeCular Technologies ApS had a better performance at low flows of 1-2 l/min and the stratifier...

  8. Stratified source-sampling techniques for Monte Carlo eigenvalue analysis.

    Mohamed, A.


    In 1995, at a conference on criticality safety, a special session was devoted to the Monte Carlo ''Eigenvalue of the World'' problem. Argonne presented a paper, at that session, in which the anomalies originally observed in that problem were reproduced in a much simplified model-problem configuration, and removed by a version of stratified source-sampling. In this paper, stratified source-sampling techniques are generalized and applied to three different Eigenvalue of the World configurations which take into account real-world statistical noise sources not included in the model problem, but which differ in the amount of neutronic coupling among the constituents of each configuration. It is concluded that, in Monte Carlo eigenvalue analysis of loosely-coupled arrays, the use of stratified source-sampling reduces the probability of encountering an anomalous result over that if conventional source-sampling methods are used. However, this gain in reliability is substantially less than that observed in the model-problem results.

  9. Stability of stratified two-phase flows in horizontal channels

    Barmak, I.; Gelfgat, A.; Vitoshkin, H.; Ullmann, A.; Brauner, N.


    Linear stability of stratified two-phase flows in horizontal channels to arbitrary wavenumber disturbances is studied. The problem is reduced to Orr-Sommerfeld equations for the stream function disturbances, defined in each sublayer and coupled via boundary conditions that account also for possible interface deformation and capillary forces. Applying the Chebyshev collocation method, the equations and interface boundary conditions are reduced to the generalized eigenvalue problems solved by standard means of numerical linear algebra for the entire spectrum of eigenvalues and the associated eigenvectors. Some additional conclusions concerning the instability nature are derived from the most unstable perturbation patterns. The results are summarized in the form of stability maps showing the operational conditions at which a stratified-smooth flow pattern is stable. It is found that for gas-liquid and liquid-liquid systems, the stratified flow with a smooth interface is stable only in confined zone of relatively low flow rates, which is in agreement with experiments, but is not predicted by long-wave analysis. Depending on the flow conditions, the critical perturbations can originate mainly at the interface (so-called "interfacial modes of instability") or in the bulk of one of the phases (i.e., "shear modes"). The present analysis revealed that there is no definite correlation between the type of instability and the perturbation wavelength.

  10. Continuous Dependence on the Density for Stratified Steady Water Waves

    Chen, Robin Ming; Walsh, Samuel


    There are two distinct regimes commonly used to model traveling waves in stratified water: continuous stratification, where the density is smooth throughout the fluid, and layer-wise continuous stratification, where the fluid consists of multiple immiscible strata. The former is the more physically accurate description, but the latter is frequently more amenable to analysis and computation. By the conservation of mass, the density is constant along the streamlines of the flow; the stratification can therefore be specified by prescribing the value of the density on each streamline. We call this the streamline density function. Our main result states that, for every smoothly stratified periodic traveling wave in a certain small-amplitude regime, there is an L ∞ neighborhood of its streamline density function such that, for any piecewise smooth streamline density function in that neighborhood, there is a corresponding traveling wave solution. Moreover, the mapping from streamline density function to wave is Lipschitz continuous in a certain function space framework. As this neighborhood includes piecewise smooth densities with arbitrarily many jump discontinues, this theorem provides a rigorous justification for the ubiquitous practice of approximating a smoothly stratified wave by a layered one. We also discuss some applications of this result to the study of the qualitative features of such waves.

  11. Survival analysis of cervical cancer using stratified Cox regression

    Purnami, S. W.; Inayati, K. D.; Sari, N. W. Wulan; Chosuvivatwong, V.; Sriplung, H.


    Cervical cancer is one of the mostly widely cancer cause of the women death in the world including Indonesia. Most cervical cancer patients come to the hospital already in an advanced stadium. As a result, the treatment of cervical cancer becomes more difficult and even can increase the death's risk. One of parameter that can be used to assess successfully of treatment is the probability of survival. This study raises the issue of cervical cancer survival patients at Dr. Soetomo Hospital using stratified Cox regression based on six factors such as age, stadium, treatment initiation, companion disease, complication, and anemia. Stratified Cox model is used because there is one independent variable that does not satisfy the proportional hazards assumption that is stadium. The results of the stratified Cox model show that the complication variable is significant factor which influent survival probability of cervical cancer patient. The obtained hazard ratio is 7.35. It means that cervical cancer patient who has complication is at risk of dying 7.35 times greater than patient who did not has complication. While the adjusted survival curves showed that stadium IV had the lowest probability of survival.

  12. Stratified spectral mixture analysis of medium resolution imagery for impervious surface mapping

    Sun, Genyun; Chen, Xiaolin; Ren, Jinchang; Zhang, Aizhu; Jia, Xiuping


    Linear spectral mixture analysis (LSMA) is widely employed in impervious surface estimation, especially for estimating impervious surface abundance in medium spatial resolution images. However, it suffers from a difficulty in endmember selection due to within-class spectral variability and the variation in the number and the type of endmember classes contained from pixel to pixel, which may lead to over or under estimation of impervious surface. Stratification is considered as a promising process to address the problem. This paper presents a stratified spectral mixture analysis in spectral domain (Sp_SSMA) for impervious surface mapping. It categorizes the entire data into three groups based on the Combinational Build-up Index (CBI), the intensity component in the color space and the Normalized Difference Vegetation Index (NDVI) values. A suitable endmember model is developed for each group to accommodate the spectral variation from group to group. The unmixing into the associated subset (or full set) of endmembers in each group can make the unmixing adaptive to the types of endmember classes that each pixel actually contains. Results indicate that the Sp_SSMA method achieves a better performance than full-set-endmember SMA and prior-knowledge-based spectral mixture analysis (PKSMA) in terms of R, RMSE and SE.

  13. Airway microbiota and pathogen abundance in age-stratified cystic fibrosis patients.

    Cox, Michael J; Allgaier, Martin; Taylor, Byron; Baek, Marshall S; Huang, Yvonne J; Daly, Rebecca A; Karaoz, Ulas; Andersen, Gary L; Brown, Ronald; Fujimura, Kei E; Wu, Brian; Tran, Diem; Koff, Jonathan; Kleinhenz, Mary Ellen; Nielson, Dennis; Brodie, Eoin L; Lynch, Susan V


    Bacterial communities in the airways of cystic fibrosis (CF) patients are, as in other ecological niches, influenced by autogenic and allogenic factors. However, our understanding of microbial colonization in younger versus older CF airways and the association with pulmonary function is rudimentary at best. Using a phylogenetic microarray, we examine the airway microbiota in age stratified CF patients ranging from neonates (9 months) to adults (72 years). From a cohort of clinically stable patients, we demonstrate that older CF patients who exhibit poorer pulmonary function possess more uneven, phylogenetically-clustered airway communities, compared to younger patients. Using longitudinal samples collected form a subset of these patients a pattern of initial bacterial community diversification was observed in younger patients compared with a progressive loss of diversity over time in older patients. We describe in detail the distinct bacterial community profiles associated with young and old CF patients with a particular focus on the differences between respective "early" and "late" colonizing organisms. Finally we assess the influence of Cystic Fibrosis Transmembrane Regulator (CFTR) mutation on bacterial abundance and identify genotype-specific communities involving members of the Pseudomonadaceae, Xanthomonadaceae, Moraxellaceae and Enterobacteriaceae amongst others. Data presented here provides insights into the CF airway microbiota, including initial diversification events in younger patients and establishment of specialized communities of pathogens associated with poor pulmonary function in older patient populations.

  14. Airway microbiota and pathogen abundance in age-stratified cystic fibrosis patients.

    Michael J Cox

    Full Text Available Bacterial communities in the airways of cystic fibrosis (CF patients are, as in other ecological niches, influenced by autogenic and allogenic factors. However, our understanding of microbial colonization in younger versus older CF airways and the association with pulmonary function is rudimentary at best. Using a phylogenetic microarray, we examine the airway microbiota in age stratified CF patients ranging from neonates (9 months to adults (72 years. From a cohort of clinically stable patients, we demonstrate that older CF patients who exhibit poorer pulmonary function possess more uneven, phylogenetically-clustered airway communities, compared to younger patients. Using longitudinal samples collected form a subset of these patients a pattern of initial bacterial community diversification was observed in younger patients compared with a progressive loss of diversity over time in older patients. We describe in detail the distinct bacterial community profiles associated with young and old CF patients with a particular focus on the differences between respective "early" and "late" colonizing organisms. Finally we assess the influence of Cystic Fibrosis Transmembrane Regulator (CFTR mutation on bacterial abundance and identify genotype-specific communities involving members of the Pseudomonadaceae, Xanthomonadaceae, Moraxellaceae and Enterobacteriaceae amongst others. Data presented here provides insights into the CF airway microbiota, including initial diversification events in younger patients and establishment of specialized communities of pathogens associated with poor pulmonary function in older patient populations.

  15. Phenomenology of two-dimensional stably stratified turbulence under large-scale forcing

    Kumar, Abhishek; Sukhatmae, Jai


    In this paper we characterize the scaling of energy spectra, and the interscale transfer of energy and enstrophy, for strongly, moderately and weakly stably stratified two-dimensional (2D) turbulence under large-scale random forcing. In the strongly stratified case, a large-scale vertically sheared horizontal flow (VSHF) co-exists with small scale turbulence. The VSHF consists of internal gravity waves and the turbulent flow has a kinetic energy (KE) spectrum that follows an approximate $k^{-3}$ scaling with zero KE flux and a robust positive enstrophy flux. The spectrum of the turbulent potential energy (PE) also approximately follows a $k^{-3}$ power-law and its flux is directed to small scales. For moderate stratification, there is no VSHF and the KE of the turbulent flow exhibits Bolgiano-Obukhov scaling that transitions from a shallow $k^{-11/5}$ form at large scales, to a steeper approximate $k^{-3}$ scaling at small scales. The entire range of scales shows a strong forward enstrophy flux, and interesti...

  16. Variance optimal sampling based estimation of subset sums

    Cohen, Edith; Kaplan, Haim; Lund, Carsten; Thorup, Mikkel


    From a high volume stream of weighted items, we want to maintain a generic sample of a certain limited size $k$ that we can later use to estimate the total weight of arbitrary subsets. This is the classic context of on-line reservoir sampling, thinking of the generic sample as a reservoir. We present a reservoir sampling scheme providing variance optimal estimation of subset sums. More precisely, if we have seen $n$ items of the stream, then for any subset size $m$, our scheme based on $k$ samples minimizes the average variance over all subsets of size $m$. In fact, the optimality is against any off-line sampling scheme tailored for the concrete set of items seen: no off-line scheme based on $k$ samples can perform better than our on-line scheme when it comes to average variance over any subset size. Our scheme has no positive covariances between any pair of item estimates. Also, our scheme can handle each new item of the stream in $O(\\log k)$ time, which is optimal even on the word RAM.

  17. Comparison of subset-based local and FE-based global digital image correlation: Theoretical error analysis and validation

    Pan, B.; Wang, B.; Lubineau, G.


    Subset-based local and finite-element-based (FE-based) global digital image correlation (DIC) approaches are the two primary image matching algorithms widely used for full-field displacement mapping. Very recently, the performances of these different DIC approaches have been experimentally investigated using numerical and real-world experimental tests. The results have shown that in typical cases, where the subset (element) size is no less than a few pixels and the local deformation within a subset (element) can be well approximated by the adopted shape functions, the subset-based local DIC outperforms FE-based global DIC approaches because the former provides slightly smaller root-mean-square errors and offers much higher computation efficiency. Here we investigate the theoretical origin and lay a solid theoretical basis for the previous comparison. We assume that systematic errors due to imperfect intensity interpolation and undermatched shape functions are negligibly small, and perform a theoretical analysis of the random errors or standard deviation (SD) errors in the displacements measured by two local DIC approaches (i.e., a subset-based local DIC and an element-based local DIC) and two FE-based global DIC approaches (i.e., Q4-DIC and Q8-DIC). The equations that govern the random errors in the displacements measured by these local and global DIC approaches are theoretically derived. The correctness of the theoretically predicted SD errors is validated through numerical translation tests under various noise levels. We demonstrate that the SD errors induced by the Q4-element-based local DIC, the global Q4-DIC and the global Q8-DIC are 4, 1.8-2.2 and 1.2-1.6 times greater, respectively, than that associated with the subset-based local DIC, which is consistent with our conclusions from previous work.

  18. Comparison of subset-based local and FE-based global digital image correlation: Theoretical error analysis and validation

    Pan, B.


    Subset-based local and finite-element-based (FE-based) global digital image correlation (DIC) approaches are the two primary image matching algorithms widely used for full-field displacement mapping. Very recently, the performances of these different DIC approaches have been experimentally investigated using numerical and real-world experimental tests. The results have shown that in typical cases, where the subset (element) size is no less than a few pixels and the local deformation within a subset (element) can be well approximated by the adopted shape functions, the subset-based local DIC outperforms FE-based global DIC approaches because the former provides slightly smaller root-mean-square errors and offers much higher computation efficiency. Here we investigate the theoretical origin and lay a solid theoretical basis for the previous comparison. We assume that systematic errors due to imperfect intensity interpolation and undermatched shape functions are negligibly small, and perform a theoretical analysis of the random errors or standard deviation (SD) errors in the displacements measured by two local DIC approaches (i.e., a subset-based local DIC and an element-based local DIC) and two FE-based global DIC approaches (i.e., Q4-DIC and Q8-DIC). The equations that govern the random errors in the displacements measured by these local and global DIC approaches are theoretically derived. The correctness of the theoretically predicted SD errors is validated through numerical translation tests under various noise levels. We demonstrate that the SD errors induced by the Q4-element-based local DIC, the global Q4-DIC and the global Q8-DIC are 4, 1.8-2.2 and 1.2-1.6 times greater, respectively, than that associated with the subset-based local DIC, which is consistent with our conclusions from previous work. © 2016 Elsevier Ltd. All rights reserved.

  19. Optimal Orderings of k-subsets for Star Identification

    Mueller, Joerg H; Simões, Luís F; Izzo, Dario


    Finding the optimal ordering of k-subsets with respect to an objective function is known to be an extremely challenging problem. In this paper we introduce a new objective for this task, rooted in the problem of star identification on spacecrafts: subsets of detected spikes are to be generated in an ordering that minimizes time to detection of a valid star constellation. We carry out an extensive analysis of the combinatorial optimization problem, and propose multiple algorithmic solutions, offering different quality-complexity trade-offs. Three main approaches are investigated: exhaustive search (branch and prune), goal-driven (greedy scene elimination, minimally intersecting subsets), and stateless algorithms which implicitly seek to satisfy the problem's goals (pattern shifting, base unrank). In practical terms, these last algorithms are found to provide satisfactory approximations to the ideal performance levels, at small computational costs.

  20. The Search for Candidate Relevant Subsets of Variables in Complex Systems.

    Villani, M; Roli, A; Filisetti, A; Fiorucci, M; Poli, I; Serra, R


    We describe a method to identify relevant subsets of variables, useful to understand the organization of a dynamical system. The variables belonging to a relevant subset should have a strong integration with the other variables of the same relevant subset, and a much weaker interaction with the other system variables. On this basis, extending previous work on neural networks, an information-theoretic measure, the dynamical cluster index, is introduced in order to identify good candidate relevant subsets. The method does not require any previous knowledge of the relationships among the system variables, but relies on observations of their values over time. We show its usefulness in several application domains, including: (i) random Boolean networks, where the whole network is made of different subnetworks with different topological relationships (independent or interacting subnetworks); (ii) leader-follower dynamics, subject to noise and fluctuations; (iii) catalytic reaction networks in a flow reactor; (iv) the MAPK signaling pathway in eukaryotes. The validity of the method has been tested in cases where the data are generated by a known dynamical model and the dynamical cluster index is applied in order to uncover significant aspects of its organization; however, it is important that it can also be applied to time series coming from field data without any reference to a model. Given that it is based on relative frequencies of sets of values, the method could be applied also to cases where the data are not ordered in time. Several indications to improve the scope and effectiveness of the dynamical cluster index to analyze the organization of complex systems are finally given.

  1. Investigating evolutionary conservation of dendritic cell subset identity and functions

    Thien-Phong eVu Manh


    Full Text Available Dendritic cells (DC were initially defined as mononuclear phagocytes with a dendritic morphology and an exquisite efficiency for naïve T cell activation. DC encompass several subsets initially identified by their expression of specific cell surface molecules and later shown to excel in distinct functions and to develop under the instruction of different transcription factors or cytokines. Very few cell surface molecules are expressed in a specific manner on any immune cell type. Hence, to identify cell types, the sole use of a small number of cell surface markers in classical flow cytometry can be deceiving. Moreover, the markers currently used to define mononuclear phagocyte subsets vary depending on the tissue and animal species studied and even between laboratories. This has led to confusion in the definition of DC subset identity and in their attribution of specific functions. There is a strong need to identify a rigorous and consensus way to define mononuclear phagocyte subsets, with precise guidelines potentially applicable throughout tissues and species. We will discuss the advantages, drawbacks and complementarities of different methodologies: cell surface phenotyping, ontogeny, functional characterization and molecular profiling. We will advocate that gene expression profiling is a very rigorous, largely unbiased and accessible method to define the identity of mononuclear phagocyte subsets, which strengthens and refines surface phenotyping. It is uniquely powerful to yield new, experimentally testable, hypotheses on the ontogeny or functions of mononuclear phagocyte subsets, their molecular regulation and their evolutionary conservation. We propose defining cell populations based on a combination of cell surface phenotyping, expression analysis of hallmark genes and robust functional assays, in order to reach a consensus and integrate faster the huge but scattered knowledge accumulated by different laboratories on different cell types

  2. Stratified randomization controls better for batch effects in 450K methylation analysis: A cautionary tale

    Olive D. Buhule


    Full Text Available Background: Batch effects in DNA methylation microarray experiments can lead to spurious results if not properly handled during the plating of samples. Methods: Two pilot studies examining the association of DNA methylation patterns across the genome with obesity in Samoan men were investigated for chip- and row-specific batch effects. For each study, the DNA of 46 obese men and 46 lean men were assayed using Illumina's Infinium HumanMethylation450 BeadChip. In the first study (Sample One, samples from obese and lean subjects were examined on separate chips. In the second study (Sample Two, the samples were balanced on the chips by lean/obese status, age group, and census region. We used methylumi, watermelon, and limma R packages, as well as ComBat, to analyze the data. Principal component analysis and linear regression were respectively employed to identify the top principal components and to test for their association with the batches and lean/obese status. To identify differentially methylated positions (DMPs between obese and lean males at each locus, we used a moderated t-test.Results: Chip effects were effectively removed from Sample Two but not Sample One. In addition, dramatic differences were observed between the two sets of DMP results. After removing'' batch effects with ComBat, Sample One had 94,191 probes differentially methylated at a q-value threshold of 0.05 while Sample Two had zero differentially methylated probes. The disparate results from Sample One and Sample Two likely arise due to the confounding of lean/obese status with chip and row batch effects.Conclusion: Even the best possible statistical adjustments for batch effects may not completely remove them. Proper study design is vital for guarding against spurious findings due to such effects.

  3. On the size of the subset partial order

    Elmasry, Amr Ahmed Abd Elmoneim


    Given a family of k sets with cardinalities S 1,S 2,⋯, S k and N=Σ k i=1S i, we show that the size of the partial order graph induced by the subset relation (called the subset graph) is O(Σ si≤B 2si+N/lgN·Σ si>Blg(s i/B)), 2 where B=lg(N/lg 2N). This implies a simpler proof to the O(N 2/lg 2N...

  4. A community study of T lymphocyte subsets and malaria parasitaemia

    Lisse, I M; Aaby, P; Whittle, H


    In a community survey of 312 children aged 3-6 years in urban Guinea-Bissau, we examined Plasmodium falciparum parasitaemia and T cell subsets. 183 children (59%) had parasites in their blood, 13 had fever > or = 37.5 degrees C, and 9 (3%) had fever and a parasite density > 5000/microL (clinical...... parasites (P = 0.024), whereas there was no association with CD8 cell percentage or the CD4/CD8 ratio. Asymptomatic parasitaemia may be an important confounder in general community studies of T cell subsets in the tropics....

  5. Magnetic Field in the Gravitationally Stratified Coronal Loops

    B. N. Dwivedi; A. K. Srivastava


    We study the effect of gravitational stratification on the estimation of magnetic fields in the coronal loops. By using the method of MHD seismology of kink waves for the estimation of magnetic field of coronal loops, we derive a new formula for the magnetic field considering the effect of gravitational stratification. The fast-kink wave is a potential diagnostic tool for the estimation of magnetic field in fluxtubes. We consider the eleven kink oscillation cases observed by TRACE between July 1998 and June 2001. We calculate magnetic field in the stratified loops (str) and compare them with the previously calculated absolute magnetic field (abs). The gravitational stratification efficiently affects the magnetic field estimation in the coronal loops as it affects also the properties of kink waves. We find ≈22% increment in the magnetic field for the smallest ( = 72 Mm) while ≈42% increment in the absolute magnetic field for the longest ( = 406 Mm) coronal loops. The magnetic fields str and abs also increase with the number density, if the loop length does not vary much. The increment in the magnetic field due to gravitational stratification is small at the lower number densities, however, it is large at the higher number densities. We find that damping time of kink waves due to phase-mixing is less in the case of gravitationally stratified loops compared to nonstratified ones. This indicates the more rapid damping of kink waves in the stratified loops. In conclusion, we find that the gravitational stratification efficiently affects the estimation of magnetic field and damping time estimation especially in the longer coronal loops.

  6. Experimental Study of Fluorine Transport Rules in Unsaturated Stratified Soil

    ZHANG Hong-mei; SU Bao-yu; LIU Peng-hua; ZHANG Wei


    With the aid of soil column test models, the transport rules of fluorine contaminants in unsaturated stratified soils are discussed. Curves of F- concentrations at different times and sites in the unsaturated stratified soil were obtained under conditions of continuous injection of fluoride contaminants and water. Based on the analysis of the actual observation data, the values between computed results and observed data were compared. It is shown that the chemical properties of fluorine ions are active. The migration process of fluorine ions in soils is complex. Because of the effect of adsorption and desorption, the curve of the fluorine ion breakthrough curve is not symmetric. Its concentration peak value at each measuring point gradually decays. The tail of the breakthrough curve is long and the process of leaching and purifying using water requires considerable time. Along with the release of OHˉ in the process of fluorine absorption, the pH value of the soil solution changed from neutral to alkalinity during the test process. The first part of the breakthrough curve fitted better than the second part. The main reason is that fluorine does not always exist in the form of fluorinions in groundwater. Given the long test time, fluorinions possibly react with other ions in the soil solution to form complex water-soluble fluorine compounds. Only the retardation factor and source-sink term have been considered in our numerical model, which may leads to errors of computed values. But as a whole the migration rules of fluorine ions are basically correct, which indicates that the established numerical model can be used to simulate the transport rules of fluorine contaminants in unsaturated stratified soils.

  7. Stratified spin-up in a sliced, square cylinder

    Munro, R. J. [Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD (United Kingdom); Foster, M. R. [Department of Mathematical Sciences, Rensselaer Polytechnic Institute, Troy, New York 12180 (United States)


    We previously reported experimental and theoretical results on the linear spin-up of a linearly stratified, rotating fluid in a uniform-depth square cylinder [M. R. Foster and R. J. Munro, “The linear spin-up of a stratified, rotating fluid in a square cylinder,” J. Fluid Mech. 712, 7–40 (2012)]. Here we extend that analysis to a “sliced” square cylinder, which has a base-plane inclined at a shallow angle α. Asymptotic results are derived that show the spin-up phase is achieved by a combination of the Ekman-layer eruptions (from the perimeter region of the cylinder's lid and base) and cross-slope-propagating stratified Rossby waves. The final, steady state limit for this spin-up phase is identical to that found previously for the uniform depth cylinder, but is reached somewhat more rapidly on a time scale of order E{sup −1/2}Ω{sup −1}/log (α/E{sup 1/2}) (compared to E{sup −1/2}Ω{sup −1} for the uniform-depth cylinder), where Ω is the rotation rate and E the Ekman number. Experiments were performed for Burger numbers, S, between 0.4 and 16, and showed that for S≳O(1), the Rossby modes are severely damped, and it is only at small S, and during the early stages, that the presence of these wave modes was evident. These observations are supported by the theory, which shows the damping factors increase with S and are numerically large for S≳O(1)

  8. Comparison of hospital-wide and age and location - stratified antibiograms of S. aureus, E. coli, and S. pneumoniae: age- and location-stratified antibiograms


    Background Antibiograms created by aggregating hospital-wide susceptibility data from diverse patients can be misleading. To demonstrate the utility of age- and location-stratified antibiograms, we compared stratified antibiograms for three common bacterial pathogens, E. coli, S. aureus, and S. pneumoniae. We created stratified antibiograms based on patient age (/=65 years), and inpatient or outpatient location using all 2009 E. coli and S. aureus, and all 2008–2009 S. pneumoniae isolates sub...

  9. Electromagnetic fields due to dipole antennas over stratified anisotropic media.

    Kong, J. A.


    Solutions to the problem of radiation of dipole antennas in the presence of a stratified anisotropic media are facilitated by decomposing a general wave field into transverse magnetic (TM) and transverse electric (TE) modes. Employing the propagation matrices, wave amplitudes in any region are related to those in any other regions. The reflection coefficients, which embed all the information about the geometrical configuration and the physical constituents of the medium, are obtained in closed form. In view of the general formulation, various special cases are discussed.

  10. Instabilities developed in stratified flows over pronounced obstacles

    Varela, J.; Araújo, M.; Bove, I.; Cabeza, C.; Usera, G.; Martí, Arturo C.; Montagne, R.; Sarasúa, L. G.


    In the present work we study numerical and experimentally the flow of a two-layer stratified fluid over a topographic obstacle. The problem reflects a wide number of oceanographic and meteorological situations, where the stratification plays an important role. We identify the different instabilities developed by studying the pycnocline deformation due to a pronounced obstacle. The numerical simulations were made using the model caffa3D.MB which works with a numerical model of Navier-Stokes equations with finite volume elements in curvilinear meshes. The experimental results are contrasted with numerical simulations. Linear stability analysis predictions are checked with particle image velocimetry (PIV) measurements.

  11. Stratified waveguide grating coupler for normal fiber incidence.

    Wang, Bin; Jiang, Jianhua; Chambers, Diana M; Cai, Jingbo; Nordin, Gregory P


    We propose a new stratified waveguide grating coupler (SWGC) to couple light from a fiber at normal incidence into a planar waveguide. SWGCs are designed to operate in the strong coupling regime without intermediate optics between the fiber and the waveguide. Two-dimensional finite-difference time-domain simulation in conjunction with microgenetic algorithm optimization shows that approximately 72% coupling efficiency is possible for fiber (core size of 8.3 microm and delta=0.36%) to slab waveguide (1.2-microm core and delta=3.1%) coupling. We show that the phase-matching and Bragg conditions are simultaneously satisfied through the fundamental leaky mode.

  12. Magnetorotational instability in weakly ionised, stratified accretion discs

    Salmeron, Roberto Aureliano; Salmeron, Raquel; Wardle, Mark


    The magnetorotational instability (MRI) (Balbus and Hawley 1991, Hawley and Balbus 1991) transports angular momentum radially outwards in accretion discs through the distortion of the magnetic field lines that connect fluid elements. In protostellar discs, low conductivity is important, especially in the inner regions (Gammie 1996, Wardle 1997). As a result, low k modes are relevant and vertical stratification is a key factor of the analysis. However, most models of the MRI in these environments have adopted either the ambipolar diffusion or resistive approximations and have not simultaneously treated stratification and Hall conductivity. We present here a linear analysis of the MRI, including the Hall effect, in a stratified disc.

  13. Enhanced charge transport kinetics in anisotropic, stratified photoanodes.

    Yazdani, Nuri; Bozyigit, Deniz; Utke, Ivo; Buchheim, Jakob; Youn, Seul Ki; Patscheider, Jörg; Wood, Vanessa; Park, Hyung Gyu


    The kinetics of charge transport in mesoporous photoanodes strongly constrains the design and power conversion efficiencies of dye sensitized solar cells (DSSCs). Here, we report a stratified photoanode design with enhanced kinetics achieved through the incorporation of a fast charge transport intermediary between the titania and charge collector. Proof of concept photoanodes demonstrate that the inclusion of the intermediary not only enhances effective diffusion coefficients but also significantly suppresses charge recombination, leading to diffusion lengths two orders of magnitude greater than in standard mesoporous titania photoanodes. The intermediary concept holds promise for higher-efficiency DSSCs.

  14. Modelling subset multivariate ARCH model via the AIC principle


    In this paper we consider the problem of identifying a parsimonious subset multivariate ARCH model based on the AIC principle. The proposed approach can reduce the number of parameters in the final ARCH specification and allows for non-constant correlations between the components. Some simulation results illustrate the viability of the proposed procedure.

  15. Directed Subset Feedback Vertex Set is Fixed-Parameter Tractable

    Chitnis, Rajesh; Hajiaghayi, MohammadTaghi; Marx, Dániel


    Given a graph $G$ and an integer $k$, the \\textsc{Feedback Vertex Set} (\\textsc{FVS}) problem asks if there is a vertex set $T$ of size at most $k$ that hits all cycles in the graph. Bodlaender (WG '91) gave the first fixed-parameter algorithm for \\textsc{FVS} in undirected graphs. The fixed-parameter tractability status of \\textsc{FVS} in directed graphs was a long-standing open problem until Chen et al. (STOC '08) showed that it is fixed-parameter tractable by giving an $4^{k}k!n^{O(1)}$ algorithm. In the subset versions of this problems, we are given an additional subset $S$ of vertices (resp. edges) and we want to hit all cycles passing through a vertex of $S$ (resp. an edge of $S$). Indeed both the edge and vertex versions are known to be equivalent in the parameterized sense. Recently the \\textsc{Subset Feedback Vertex Set} in undirected graphs was shown to be FPT by Cygan et al. (ICALP '11) and Kakimura et al. (SODA '12). We generalize the result of Chen et al. (STOC '08) by showing that \\textsc{Subset...

  16. Poincare series of subsets of affine Weyl groups

    Viswanath, Sankaran


    In this note, we identify a natural class of subsets of affine Weyl groups whose Poincare series are rational functions. This class includes the sets of minimal coset representatives of reflection subgroups. As an application, we construct a generalization of the classical length-descent generating function, and prove its rationality.

  17. Effects of a Simulated Tennis Match on Lymphocyte Subset Measurements

    Schafer, Mark; Kell, Holly; Navalta, James; Tibana, Ramires; Lyons, Scott; Arnett, Scott


    Tennis is an activity requiring both endurance and anaerobic components, which could have immunosuppressive effects postexercise. Purpose: The purpose of this investigation was to determine the effect of a simulated tennis match on apoptotic and migratory markers on lymphocyte subsets. Method: Male high school (n = 5) and college (n = 3) tennis…

  18. Combinatorial Identities Via Phi Functions and Relatively Prime Subsets

    Bachraoui, Mohamed El


    Let $n$ be a positive integer and let $A$ be nonempty finite set of positive integers. We say that $A$ is relatively prime if $\\gcd(A) =1$ and that $A$ is relatively prime to $n$ if $\\gcd(A,n)=1$. In this work we count the number of nonempty subsets of $A$ which are relatively prime and the number of nonempty subsets of $A$ which are relatively prime to $n$. Related formulas are also obtained for the number of such subsets having some fixed cardinality. This extends previous work for the cases where $A$ is an interval or a set in arithmetic progression. Applications include: a) An exact formula is obtained for the number of elements of $A$ which are co-prime to $n$; note that this number is $\\phi(n)$ if $A=[1,n]$. b) Algebraic characterizations are found for a nonempty finite set of positive integers to have elements which are all pairwise co-prime and consequently a formula is given for the number of nonempty subsets of $A$ whose elements are pairwise co-prime. c) We provide combinatorial formulas involving ...

  19. Clustering, Seriation, and Subset Extraction of Confusion Data

    Brusco, Michael J.; Steinley, Douglas


    The study of confusion data is a well established practice in psychology. Although many types of analytical approaches for confusion data are available, among the most common methods are the extraction of 1 or more subsets of stimuli, the partitioning of the complete stimulus set into distinct groups, and the ordering of the stimulus set. Although…


    Lan Peng; Liu Ju; Gu Bo; Zhang Wei


    A new receive antenna subset selection algorithm with low complexity for wireless Multiple-Input Multiple-Output (MIMO) systems is proposed, which is based on the orthogonal components of the channel matrix. Larger capacity is achieved compared with the existing antenna selection methods. Simulation results of quasi-static flat fading channel demonstrate the significant performance of the proposed selection algorithm.

  1. A-Stratified Computerized Adaptive Testing with Unequal Item Exposure across Strata.

    Deng, Hui; Chang, Hua-Hua

    The purpose of this study was to compare a proposed revised a-stratified, or alpha-stratified, USTR method of test item selection with the original alpha-stratified multistage computerized adaptive testing approach (STR) and the use of maximum Fisher information (FSH) with respect to test efficiency and item pool usage using simulated computerized…

  2. Penetrative convection in stratified fluids: velocity and temperature measurements

    M. Moroni


    Full Text Available The flux through the interface between a mixing layer and a stable layer plays a fundamental role in characterizing and forecasting the quality of water in stratified lakes and in the oceans, and the quality of air in the atmosphere. The evolution of the mixing layer in a stably stratified fluid body is simulated in the laboratory when "Penetrative Convection" occurs. The laboratory model consists of a tank filled with water and subjected to heating from below. The methods employed to detect the mixing layer growth were thermocouples for temperature data and two image analysis techniques, namely Laser Induced Fluorescence (LIF and Feature Tracking (FT. LIF allows the mixing layer evolution to be visualized. Feature Tracking is used to detect tracer particle trajectories moving within the measurement volume. Pollutant dispersion phenomena are naturally described in the Lagrangian approach as the pollutant acts as a tag of the fluid particles. The transilient matrix represents one of the possible tools available for quantifying particle dispersion during the evolution of the phenomenon.


    Bobileva Tatiana Nikolaevna


    Full Text Available Almost all subsurface rocks used as foundations for various types of structures are stratified. Such heterogeneity may cause specific behaviour of the materials under strain. Differential equations describing the behaviour of such materials contain rapidly fluctuating coefficients, in view of this, solution of such equations is more time-consuming when using today’s computers. The method of asymptotic averaging leads to getting homogeneous medium under study to averaged equations with fixed factors. The present article is concerned with stratified soil mass consisting of pair-wise alternative isotropic elastic layers. In the results of elastic modules averaging, the present soil mass with horizontal rock stratification is simulated by homogeneous transversal-isotropic half-space with isotropy plane perpendicular to the standing axis. Half-space is loosened by a vertical alveole of circular cross-section, and virgin ground is under its own weight. For horizontal parting planes of layers, the following two types of surface conditions are set: ideal contact and backlash without cleavage. For homogeneous transversal-isotropic half-space received with a vertical alveole, the analytical solution of S.G. Lekhnitsky, well known in scientific papers, is used. The author gives expressions for stress components and displacements in soil mass for different marginal conditions on the alveole surface. Such research problems arise when constructing and maintaining buildings and when composite materials are used.

  4. Stability of steam-water countercurrent stratified flow

    Lee, S C


    Two flow instabilities which limit the normal condensation processes in countercurrent stratified steam-water flow have been identified experimentally: flooding and condensation-induced waterhammer. In order to initiate condensation-induced waterhammer in nearly horizontal or moderately-inclined steam/subcooled-water flow, two conditions, the appearance of a wavy interface and complete condensation of the incoming steam, are necessary. Analyses of these conditions are performed on a basis of flow stability and heat transfer considerations. Flooding data for several inclinations and channel heights are collected. Effects of condensation, inclination angle and channel height on the flooding characteristics are discussed. An envelope theory for the onset of flooding in inclined stratified flow is developed, which agrees well with the experimental data. Some empirical information on basic flow parameters, such as mean film thickness and interfacial friction factor required for this theory are measured. The previous viewpoints on flooding appear not to conflict with the present experimental data in nearly horizontal flow but the flooding phenomena in nearly vertical flow appear to be more complicated than those described by these viewpoints because of liquid droplet entrainment.

  5. Strongly Stratified Turbulence Wakes and Mixing Produced by Fractal Wakes

    Dimitrieva, Natalia; Redondo, Jose Manuel; Chashechkin, Yuli; Fraunie, Philippe; Velascos, David


    This paper describes Shliering and Shadowgraph experiments of the wake induced mixing produced by tranversing a vertical or horizontal fractal grid through the interfase between two miscible fluids at low Atwood and Reynolds numbers. This is a configuration design to models the mixing across isopycnals in stably-stratified flows in many environmental relevant situations (either in the atmosphere or in the ocean. The initial unstable stratification is characterized by a reduced gravity: g' = gΔρ ρ where g is gravity, Δρ being the initial density step and ρ the reference density. Here the Atwood number is A = g' _ 2 g . The topology of the fractal wake within the strong stratification, and the internal wave field produces both a turbulent cascade and a wave cascade, with frecuen parametric resonances, the envelope of the mixing front is found to follow a complex non steady 3rd order polinomial function with a maximum at about 4-5 Brunt-Vaisalla non-dimensional time scales: t/N δ = c1(t/N) + c2g Δρ ρ (t/N)2 -c3(t/N)3. Conductivity probes and Shliering and Shadowgraph visual techniques, including CIV with (Laser induced fluorescence and digitization of the light attenuation across the tank) are used in order to investigate the density gradients and the three-dimensionality of the expanding and contracting wake. Fractal analysis is also used in order to estimate the fastest and slowest growing wavelengths. The large scale structures are observed to increase in wave-length as the mixing progresses, and the processes involved in this increase in scale are also examined.Measurements of the pointwise and horizontally averaged concentrations confirm the picture obtained from past flow visualization studies. They show that the fluid passes through the mixing region with relatively small amounts of molecular mixing,and the molecular effects only dominate on longer time scales when the small scales have penetrated through the large scale structures. The Non

  6. On the Security of Millimeter Wave Vehicular Communication Systems Using Random Antenna Subsets

    Eltayeb, Mohammed E.


    Millimeter wave (mmWave) vehicular communication systems have the potential to improve traffic efficiency and safety. Lack of secure communication links, however, may lead to a formidable set of abuses and attacks. To secure communication links, a physical layer precoding technique for mmWave vehicular communication systems is proposed in this paper. The proposed technique exploits the large dimensional antenna arrays available at mmWave systems to produce direction dependent transmission. This results in coherent transmission to the legitimate receiver and artificial noise that jams eavesdroppers with sensitive receivers. Theoretical and numerical results demonstrate the validity and effectiveness of the proposed technique and show that the proposed technique provides high secrecy throughput when compared to conventional array and switched array transmission techniques.

  7. A reassessment of IgM memory subsets in humans

    Bagnara, Davide; Squillario, Margherita; Kipling, David; Mora, Thierry; Walczak, Aleksandra M.; Da Silva, Lucie; Weller, Sandra; Dunn-Walters, Deborah K.; Weill, Jean-Claude; Reynaud, Claude-Agnès


    From paired blood and spleen samples from three adult donors we performed high-throughput V-h sequencing of human B-cell subsets defined by IgD and CD27 expression: IgD+CD27+ (“MZ”), IgD−CD27+(“memory”, including IgM (“IgM-only”), IgG and IgA) and IgD−CD27− cells (“double-negative”, including IgM, IgG and IgA). 91,294 unique sequences clustered in 42,670 clones, revealing major clonal expansions in each of these subsets. Among these clones, we further analyzed those shared sequences from different subsets or tissues for Vh-gene mutation, H-CDR3-length, and Vh/Jh usage, comparing these different characteristics with all sequences from their subset of origin, for which these parameters constitute a distinct signature. The IgM-only repertoire profile differed notably from that of MZ B cells by a higher mutation frequency, and lower Vh4 and higher Jh6 gene usage. Strikingly, IgM sequences from clones shared between the MZ and the memory IgG/IgA compartments showed a mutation and repertoire profile of IgM-only and not of MZ B cells. Similarly, all IgM clonal relationships (between MZ, IgM-only, and double-negative compartments) involved sequences with the characteristics of IgM-only B cells. Finally, clonal relationships between tissues suggested distinct recirculation characteristics between MZ and switched B cells. The “IgM-only” subset (including cells with its repertoire signature but higher IgD or lower CD27 expression levels) thus appear as the only subset showing precursor-product relationships with CD27+ switched memory B cells, indicating that they represent germinal center-derived IgM memory B cells, and that IgM memory and MZ B cells constitute two distinct entities. PMID:26355154

  8. Implementing Modifed Burg Algorithms in Multivariate Subset Autoregressive Modeling

    A. Alexandre Trindade


    Full Text Available The large number of parameters in subset vector autoregressive models often leads one to procure fast, simple, and efficient alternatives or precursors to maximum likelihood estimation. We present the solution of the multivariate subset Yule-Walker equations as one such alternative. In recent work, Brockwell, Dahlhaus, and Trindade (2002, show that the Yule-Walker estimators can actually be obtained as a special case of a general recursive Burg-type algorithm. We illustrate the structure of this Algorithm, and discuss its implementation in a high-level programming language. Applications of the Algorithm in univariate and bivariate modeling are showcased in examples. Univariate and bivariate versions of the Algorithm written in Fortran 90 are included in the appendix, and their use illustrated.

  9. Myeloid-derived suppressor cell heterogeneity and subset definition.

    Peranzoni, Elisa; Zilio, Serena; Marigo, Ilaria; Dolcetti, Luigi; Zanovello, Paola; Mandruzzato, Susanna; Bronte, Vincenzo


    Myeloid derived suppressor cells (MDSCs) are defined in mice on the basis of CD11b and Gr-1 marker expression and the functional ability to inhibit T lymphocyte activation. Nevertheless the term 'heterogeneous' remains the first, informal feature commonly attributed to this population. It is clear that CD11b(+)Gr-1(+) cells are part of a myeloid macropopulation, which comprises at least two subsets of polymorphonuclear and monocytic cells with different immunosuppressive properties. While recent literature shows substantial agreement on the immunoregulatory property of the monocytic MDSC subset, there is still contrasting evidence on the role of the granulocytic fraction. Moreover, this dichotomy holds true for human MDSCs. We attempt here to summarize conflicting findings in the field and provide some possible, unifying explanations.

  10. Variable selection with stepwise and best subset approaches.

    Zhang, Zhongheng


    While purposeful selection is performed partly by software and partly by hand, the stepwise and best subset approaches are automatically performed by software. Two R functions stepAIC() and bestglm() are well designed for stepwise and best subset regression, respectively. The stepAIC() function begins with a full or null model, and methods for stepwise regression can be specified in the direction argument with character values "forward", "backward" and "both". The bestglm() function begins with a data frame containing explanatory variables and response variables. The response variable should be in the last column. Varieties of goodness-of-fit criteria can be specified in the IC argument. The Bayesian information criterion (BIC) usually results in more parsimonious model than the Akaike information criterion.

  11. Subsets of human natural killer cells and their regulatory effects

    Fu, Binqing; Tian, Zhigang; Wei, Haiming


    Human natural killer (NK) cells have distinct functions as NKtolerant, NKcytotoxic and NKregulatory cells and can be divided into different subsets based on the relative expression of the surface markers CD27 and CD11b. CD27+ NK cells, which are abundant cytokine producers, are numerically in the minority in human peripheral blood but constitute the large population of NK cells in cord blood, spleen, tonsil and decidua tissues. Recent data suggest that these NK cells may have immunoregulatory properties under certain conditions. In this review, we will focus on these new NK cell subsets and discuss how regulatory NK cells may serve as rheostats or sentinels in controlling inflammation and maintaining immune homeostasis in various organs. PMID:24303897

  12. Invited Review. Combustion instability in spray-guided stratified-charge engines. A review

    Fansler, Todd D. [Univ. of Wisconsin, Madison, WI (United States); Reuss, D. L. [Univ. of Michigan, Ann Arbor, MI (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sick, V. [Univ. of Michigan, Ann Arbor, MI (United States); Dahms, R. N. [Sandia National Lab. (SNL-CA), Livermore, CA (United States)


    Our article reviews systematic research on combustion instabilities (principally rare, random misfires and partial burns) in spray-guided stratified-charge (SGSC) engines operated at part load with highly stratified fuel -air -residual mixtures. Results from high-speed optical imaging diagnostics and numerical simulation provide a conceptual framework and quantify the sensitivity of ignition and flame propagation to strong, cyclically varying temporal and spatial gradients in the flow field and in the fuel -air -residual distribution. For SGSC engines using multi-hole injectors, spark stretching and locally rich ignition are beneficial. Moreover, combustion instability is dominated by convective flow fluctuations that impede motion of the spark or flame kernel toward the bulk of the fuel, coupled with low flame speeds due to locally lean mixtures surrounding the kernel. In SGSC engines using outwardly opening piezo-electric injectors, ignition and early flame growth are strongly influenced by the spray's characteristic recirculation vortex. For both injection systems, the spray and the intake/compression-generated flow field influence each other. Factors underlying the benefits of multi-pulse injection are identified. Finally, some unresolved questions include (1) the extent to which piezo-SGSC misfires are caused by failure to form a flame kernel rather than by flame-kernel extinction (as in multi-hole SGSC engines); (2) the relative contributions of partially premixed flame propagation and mixing-controlled combustion under the exceptionally late-injection conditions that permit SGSC operation on E85-like fuels with very low NOx and soot emissions; and (3) the effects of flow-field variability on later combustion, where fuel-air-residual mixing within the piston bowl becomes important.

  13. Phenomenology of two-dimensional stably stratified turbulence under large-scale forcing

    Kumar, Abhishek


    In this paper, we characterise the scaling of energy spectra, and the interscale transfer of energy and enstrophy, for strongly, moderately and weakly stably stratified two-dimensional (2D) turbulence, restricted in a vertical plane, under large-scale random forcing. In the strongly stratified case, a large-scale vertically sheared horizontal flow (VSHF) coexists with small scale turbulence. The VSHF consists of internal gravity waves and the turbulent flow has a kinetic energy (KE) spectrum that follows an approximate k−3 scaling with zero KE flux and a robust positive enstrophy flux. The spectrum of the turbulent potential energy (PE) also approximately follows a k−3 power-law and its flux is directed to small scales. For moderate stratification, there is no VSHF and the KE of the turbulent flow exhibits Bolgiano–Obukhov scaling that transitions from a shallow k−11/5 form at large scales, to a steeper approximate k−3 scaling at small scales. The entire range of scales shows a strong forward enstrophy flux, and interestingly, large (small) scales show an inverse (forward) KE flux. The PE flux in this regime is directed to small scales, and the PE spectrum is characterised by an approximate k−1.64 scaling. Finally, for weak stratification, KE is transferred upscale and its spectrum closely follows a k−2.5 scaling, while PE exhibits a forward transfer and its spectrum shows an approximate k−1.6 power-law. For all stratification strengths, the total energy always flows from large to small scales and almost all the spectral indicies are well explained by accounting for the scale-dependent nature of the corresponding flux.

  14. Hydrodynamics of stratified epithelium: steady state and linearized dynamics

    Yeh, Wei-Ting


    A theoretical model for stratified epithelium is presented. The viscoelastic properties of the tissue is assumed to be dependent on the spatial distribution of proliferative and differentiated cells. Based on this assumption, a hydrodynamic description for tissue dynamics at long-wavelength, long-time limit is developed, and the analysis reveals important insight for the dynamics of an epithelium close to its steady state. When the proliferative cells occupy a thin region close to the basal membrane, the relaxation rate towards the steady state is enhanced by cell division and cell apoptosis. On the other hand, when the region where proliferative cells reside becomes sufficiently thick, a flow induced by cell apoptosis close to the apical surface could enhance small perturbations. This destabilizing mechanism is general for continuous self-renewal multi-layered tissues, it could be related to the origin of certain tissue morphology and developing pattern.

  15. Hydrodynamics of stratified epithelium: Steady state and linearized dynamics

    Yeh, Wei-Ting; Chen, Hsuan-Yi


    A theoretical model for stratified epithelium is presented. The viscoelastic properties of the tissue are assumed to be dependent on the spatial distribution of proliferative and differentiated cells. Based on this assumption, a hydrodynamic description of tissue dynamics at the long-wavelength, long-time limit is developed, and the analysis reveals important insights into the dynamics of an epithelium close to its steady state. When the proliferative cells occupy a thin region close to the basal membrane, the relaxation rate towards the steady state is enhanced by cell division and cell apoptosis. On the other hand, when the region where proliferative cells reside becomes sufficiently thick, a flow induced by cell apoptosis close to the apical surface enhances small perturbations. This destabilizing mechanism is general for continuous self-renewal multilayered tissues; it could be related to the origin of certain tissue morphology, tumor growth, and the development pattern.

  16. Local Radiation MHD Instabilities in Magnetically Stratified Media

    Tao, Ted


    We study local radiation magnetohydrodynamic instabilities in static, optically thick, vertically stratified media with constant flux mean opacity. We include the effects of vertical gradients in a horizontal background magnetic field. Assuming rapid radiative diffusion, we use the zero gas pressure limit as an entry point for investigating the coupling between the photon bubble instability and the Parker instability. Apart from factors that depend on wavenumber orientation, the Parker instability exists for wavelengths longer than a characteristic wavelength lambda_{tran}, while photon bubbles exist for wavelengths shorter than lambda_{tran}. The growth rate in the Parker regime is independent of the orientation of the horizontal component of the wavenumber when radiative diffusion is rapid, but the range of Parker-like wavenumbers is extended if there exists strong horizontal shear between field lines (i.e. horizontal wavenumber perpendicular to the magnetic field). Finite gas pressure introduces an additio...

  17. Short-wave vortex instability in stratified flow

    Bovard, Luke


    In this paper we investigate a new instability of the Lamb-Chaplygin dipole in a stratified fluid. Through numerical linear stability analysis, a secondary peak in the growth rate emerges at vertical scales about an order of magnitude smaller than the buoyancy scale $L_{b}=U/N$ where $U$ is the characteristic velocity and $N$ is the Brunt-V\\"{a}is\\"{a}l\\"{a} frequency. This new instability exhibits a growth rate that is similar to, and even exceeds, that of the zigzag instability, which has the characteristic length of the buoyancy scale. This instability is investigated for a wide range of Reynolds $Re=2000-20000$ and horizontal Froude numbers $F_{h}=0.05-0.2$, where $F_{h}=U/NR$, $Re=UR/\

  18. Internal combustion engine using premixed combustion of stratified charges

    Marriott, Craig D.; Reitz, Rolf D. (Madison, WI


    During a combustion cycle, a first stoichiometrically lean fuel charge is injected well prior to top dead center, preferably during the intake stroke. This first fuel charge is substantially mixed with the combustion chamber air during subsequent motion of the piston towards top dead center. A subsequent fuel charge is then injected prior to top dead center to create a stratified, locally richer mixture (but still leaner than stoichiometric) within the combustion chamber. The locally rich region within the combustion chamber has sufficient fuel density to autoignite, and its self-ignition serves to activate ignition for the lean mixture existing within the remainder of the combustion chamber. Because the mixture within the combustion chamber is overall premixed and relatively lean, NO.sub.x and soot production are significantly diminished.

  19. A study of stratified gas-liquid pipe flow

    Johnson, George W.


    This work includes both theoretical modelling and experimental observations which are relevant to the design of gas condensate transport lines. Multicomponent hydrocarbon gas mixtures are transported in pipes over long distances and at various inclinations. Under certain circumstances, the heavier hydrocarbon components and/or water vapour condense to form one or more liquid phases. Near the desired capacity, the liquid condensate and water is efficiently transported in the form of a stratified flow with a droplet field. During operating conditions however, the flow rate may be reduced allowing liquid accumulation which can create serious operational problems due to large amounts of excess liquid being expelled into the receiving facilities during production ramp-up or even in steady production in severe cases. In particular, liquid tends to accumulate in upward inclined sections due to insufficient drag on the liquid from the gas. To optimize the transport of gas condensates, a pipe diameters should be carefully chosen to account for varying flow rates and pressure levels which are determined through the knowledge of the multiphase flow present. It is desirable to have a reliable numerical simulation tool to predict liquid accumulation for various flow rates, pipe diameters and pressure levels which is not presently accounted for by industrial flow codes. A critical feature of the simulation code would include the ability to predict the transition from small liquid accumulation at high flow rates to large liquid accumulation at low flow rates. A semi-intermittent flow regime of roll waves alternating with a partly backward flowing liquid film has been observed experimentally to occur for a range of gas flow rates. Most of the liquid is transported in the roll waves. The roll wave regime is not well understood and requires fundamental modelling and experimental research. The lack of reliable models for this regime leads to inaccurate prediction of the onset of

  20. Turbulent reconnection of magnetic bipoles in stratified turbulence

    Jabbari, Sarah; Mitra, Dhrubaditya; Kleeorin, Nathan; Rogachevskii, Igor


    We consider strongly stratified forced turbulence in a plane-parallel layer with helicity and corresponding large-scale dynamo action in the lower part and nonhelical turbulence in the upper. The magnetic field is found to develop strongly concentrated bipolar structures near the surface. They form elongated bands with a sharp interface between opposite polarities. Unlike earlier experiments with imposed magnetic field, the inclusion of rotation does not strongly suppress the formation of these structures. We perform a systematic numerical study of this phenomenon by varying magnetic Reynolds number, scale separation ratio, and Coriolis number. We also focus on the formation of the current sheet between bipolar regions where reconnection of oppositely oriented field lines occurs. We determine the reconnection rate by measuring either the inflow velocity in the vicinity of the current sheet or by measuring the electric field in the reconnection region. We demonstrate that for small Lundquist number, S1000, the...

  1. Direct simulation of the stably stratified turbulent Ekman layer

    Coleman, G. N.; Ferziger, J. H.; Spalart, P. R.


    The Navier-Stokes equations and the Boussinesq approximation were used to compute a 3D time-dependent turbulent flow in the stably stratified Ekman layer over a smooth surface. The simulation data are found to be in very good agreement with atmospheric measurements when nondimensionalized according to Nieuwstadt's local scaling scheme. Results suggest that, when Reynolds number effects are taken into account, the 'constant Froud number' stable layer model (Brost and Wyngaard, 1978) and the 'shearing length' stable layer model (Hunt, 1985) for the dissipitation rate of turbulent kinetic energy are both valid. It is concluded that there is good agreement between the direct numerical simulation results and large-eddy simulation results obtained by Mason and Derbyshire (1990).

  2. Inertial modes of non-stratified superfluid neutron stars

    Prix, R; Andersson, N


    We present results concerning adiabatic inertial-mode oscillations of non-stratified superfluid neutron stars in Newtonian gravity, using the anelastic and slow-rotation approximations. We consider a simple two-fluid model of a superfluid neutron star, where one fluid consists of the superfluid neutrons and the second fluid contains all the comoving constituents (protons, electrons). The two fluids are assumed to be ``free'' in the sense that vortex-mediated forces like mutual friction or pinning are absent, but they can be coupled by the equation of state, in particular by entrainment. The stationary background consists of the two fluids rotating uniformly around the same axis with potentially different rotation rates. We study the special cases of co-rotating backgrounds, vanishing entrainment, and the purely toroidal r-modes, analytically. We calculate numerically the eigenfunctions and frequencies of inertial modes in the general case of non co-rotating backgrounds, and study their dependence on the relat...

  3. Magnetorotational instability in stratified, weakly ionised accretion discs

    Salmeron, Roberto Aureliano; Salmeron, Raquel; Wardle, Mark


    We present a linear analysis of the vertical structure and growth of the magnetorotational instability in stratified, weakly ionised accretion discs, such as protostellar and quiescent dwarf novae systems. The method includes the effects of the magnetic coupling, the conductivity regime of the fluid and the strength of the magnetic field, which is initially vertical. The conductivity is treated as a tensor and assumed constant with height. We obtained solutions for the structure and growth rate of global unstable modes for different conductivity regimes, strengths of the initial magnetic field and coupling between ionised and neutral components of the fluid. The envelopes of short-wavelenght perturbations are determined by the action of competing local growth rates at different heights, driven by the vertical stratification of the disc. Ambipolar diffusion perturbations peak consistently higher above the midplane than modes including Hall conductivity. For weak coupling, perturbations including the Hall effec...

  4. Second order closure for stratified convection: bulk region and overshooting

    Biferale, L; Sbragaglia, M; Scagliarini, A; Toschi, F; Tripiccione, R


    The parameterization of small-scale turbulent fluctuations in convective systems and in the presence of strong stratification is a key issue for many applied problems in oceanography, atmospheric science and planetology. In the presence of stratification, one needs to cope with bulk turbulent fluctuations and with inversion regions, where temperature, density -or both- develop highly non-linear mean profiles due to the interactions between the turbulent boundary layer and the unmixed -stable- flow above/below it. We present a second order closure able to cope simultaneously with both bulk and boundary layer regions, and we test it against high-resolution state-of-the-art 2D numerical simulations in a convective and stratified belt for values of the Rayleigh number, up to Ra = 10^9. Data are taken from a Rayleigh-Taylor system confined by the existence of an adiabatic gradient.

  5. Subset Feature Learning for Fine-Grained Category Classification

    Ge, Zongyuan; McCool, Christopher; Sanderson, Conrad; Corke, Peter


    Fine-grained categorisation has been a challenging problem due to small inter-class variation, large intra-class variation and low number of training images. We propose a learning system which first clusters visually similar classes and then learns deep convolutional neural network features specific to each subset. Experiments on the popular fine-grained Caltech-UCSD bird dataset show that the proposed method outperforms recent fine-grained categorisation methods under the most difficult sett...

  6. Subset Simulation Method for Rare Event Estimation: An Introduction


    This paper provides a detailed introductory description of Subset Simulation, an advanced stochastic simulation method for estimation of small probabilities of rare failure events. A simple and intuitive derivation of the method is given along with the discussion on its implementation. The method is illustrated with several easy-to-understand examples. For demonstration purposes, the MATLAB code for the considered examples is provided. The reader is assumed to be familiar only with elementary...

  7. Torsion Points on an Algebraic Subset of an Affine Torus

    Hironaka, E


    Work of Laurent and Sarnak, following a conjecture of Lang, shows that the number of torsion points of order n on an algebraic subset of an affine complex torus is polynomial periodic. In this paper, we find bounds on the degree and period of this number as a function of n. Some examples, including the number of n torsion points on Fermat curves, are computed to illustrate the methods.

  8. Cortistain is expressed in a distinct subset of cortical interneurons

    Lecea Flores de Lemus, Luis de; Río Fernández, José Antonio del; Alcántara Horrillo, Soledad; Criado, José R.; Morales, Marisela; Danielson, Patria E.; Henriksen, Steven J.; Soriano García, Eduardo; Sutcliffe, J. Gregor


    Cortistatin is a presumptive neuropeptide that shares 11 of its 14 amino acids with somatostatin. In contrast to somatostatin, administration of cortistatin into the rat brain ventricles specifically enhances slow wave sleep, apparently by antagonizing the effects of acetylcholine on cortical excitability. Here we show that preprocortistatin mRNA is expressed in a subset of GABAergic cells in the cortex and hippocampus that partially overlap with those containing somatostatin. A significant p...

  9. Differentiable structure of ω-subsets of symplectic groups


    In this paper, we study the differentiable structure of theω-subset of Sp(2n), which is formed by all matrices in Sp(2n) possessing ω as an eigenvalue, for ω on the unit circle in the complex plane. Based on this result the ω-index theory parametrized by all ω on the unit circle for arbitrary symplectic paths is defined.

  10. Oxygenation of Stratified Reservoir Using Air Bubble Plume

    Schladow, S. G.


    Excess nutrients loading from urban area and watershed into lakes and reservoirs increases the content of organic matter, which, through decomposition, needs increased dissolve oxygen (DO). Many eutrophic reservoirs and lakes cannot meet the DO requirement during stratified season and suffers from the hypolimnetic anoxia. As a result, benthic sediment produces anoxic products such as methane, hydrogen sulphide, ammonia, iron, manganese, and phosphorus. In order to address the hypolimnetic anoxia, oxygen is artificially supplied into reservoir using an aeration system (i.e., bubbler). The most common result of lake/reservoir aeration is to destratify the reservoir so that the water body may completely mix under natural phenomena and remain well oxygenated throughout. Other advantages of destratification are: (1) allows warm- water fish to inhabit the entire reservoir, (2) suppress the nutrient release from sediment, and (3) decreases the algal growth by sending them to the darker zone. A one-dimensional reservoir-bubbler model is developed and applied to examine the effects of an aeration system on mixing and dissolved oxygen dynamics in the Upper Peirce Reservoir, Singapore. After introduction of the aeration system in the reservoir, it was found that the hypolimnetic DO increased significantly, and the concentration of algae, soluble manganese and iron substantially reduced. It is found that the reservoir-bubbler model predicts the mixing (temperature as mixing parameter) and dissolved oxygen concentration in the reservoir with acceptable accuracy. It is shown in terms of bubbler mechanical efficiency (i.e., operating cost) and total DO contribution from the aeration system into the reservoir that the selections of airflow rate per diffuser, air bubble radius, and total number of diffusers are important design criteria of a bubbler system. However, the overall bubbler design also depends on the reservoir size and stratified area of interest, ambient climate, and

  11. Visualization periodic flows in a continuously stratified fluid.

    Bardakov, R.; Vasiliev, A.


    To visualize the flow pattern of viscous continuously stratified fluid both experimental and computational methods were developed. Computational procedures were based on exact solutions of set of the fundamental equations. Solutions of the problems of flows producing by periodically oscillating disk (linear and torsion oscillations) were visualized with a high resolutions to distinguish small-scale the singular components on the background of strong internal waves. Numerical algorithm of visualization allows to represent both the scalar and vector fields, such as velocity, density, pressure, vorticity, stream function. The size of the source, buoyancy and oscillation frequency, kinematic viscosity of the medium effects were traced in 2D an 3D posing problems. Precision schlieren instrument was used to visualize the flow pattern produced by linear and torsion oscillations of strip and disk in a continuously stratified fluid. Uniform stratification was created by the continuous displacement method. The buoyancy period ranged from 7.5 to 14 s. In the experiments disks with diameters from 9 to 30 cm and a thickness of 1 mm to 10 mm were used. Different schlieren methods that are conventional vertical slit - Foucault knife, vertical slit - filament (Maksoutov's method) and horizontal slit - horizontal grating (natural "rainbow" schlieren method) help to produce supplementing flow patterns. Both internal wave beams and fine flow components were visualized in vicinity and far from the source. Intensity of high gradient envelopes increased proportionally the amplitude of the source. In domains of envelopes convergence isolated small scale vortices and extended mushroom like jets were formed. Experiments have shown that in the case of torsion oscillations pattern of currents is more complicated than in case of forced linear oscillations. Comparison with known theoretical model shows that nonlinear interactions between the regular and singular flow components must be taken

  12. T Helper Cell Subsets in Clinical Manifestations of Psoriasis

    Marco Diani


    Full Text Available Psoriasis is a chronic inflammatory skin disease, which is associated with systemic inflammation and comorbidities, such as psoriatic arthritis and cardiovascular diseases. The autoimmune nature of psoriasis has been established only recently, conferring a central role to epidermal CD8 T cells recognizing self-epitopes in the initial phase of the disease. Different subsets of helper cells have also been reported as key players in the psoriasis pathogenesis. Here, we reviewed the knowledge on the role of each subset in the psoriatic cascade and in the different clinical manifestations of the disease. We will discuss the role of Th1 and Th17 cells in the initiation and in the amplification phase of cutaneous inflammation. Moreover, we will discuss the recently proposed role of tissue resident Th22 cells in disease memory in sites of recurrent psoriasis and the possible involvement of Th9 cells. Finally, we will discuss the hypothesis of a link between T helper cell subsets recirculating from the skin and the systemic manifestations of psoriasis.

  13. T Helper Cell Subsets in Clinical Manifestations of Psoriasis

    Diani, Marco; Altomare, Gianfranco


    Psoriasis is a chronic inflammatory skin disease, which is associated with systemic inflammation and comorbidities, such as psoriatic arthritis and cardiovascular diseases. The autoimmune nature of psoriasis has been established only recently, conferring a central role to epidermal CD8 T cells recognizing self-epitopes in the initial phase of the disease. Different subsets of helper cells have also been reported as key players in the psoriasis pathogenesis. Here, we reviewed the knowledge on the role of each subset in the psoriatic cascade and in the different clinical manifestations of the disease. We will discuss the role of Th1 and Th17 cells in the initiation and in the amplification phase of cutaneous inflammation. Moreover, we will discuss the recently proposed role of tissue resident Th22 cells in disease memory in sites of recurrent psoriasis and the possible involvement of Th9 cells. Finally, we will discuss the hypothesis of a link between T helper cell subsets recirculating from the skin and the systemic manifestations of psoriasis. PMID:27595115

  14. Preparation of Internal Quality Control Material for Lymphocyte Subset Analysis.

    Roh, Eun Youn; Shin, Sue; Yoon, Jong Hyun; Oh, Sohee; Park, Kyoung Un; Lee, Nuri; Song, Eun Young


    Lymphocyte subset analysis is widely used in clinical laboratories, and more than two levels of daily QC materials are required for reliable results. Commercially available, expensive QC materials have short shelf lives and may not be suitable in resource-poor settings. We compared different methods for preparing homemade QC material, including fixation with 1%, 2%, or 4% paraformaldehyde (PFA); freezing with 10% dimethylsulfoxide (DMSO), 0.1% bovine serum albumin-phosphate buffered saline, or after ethanolic dehydration; and using cryopreservation temperatures of -20°C, -80°C, or -196°C. We found an optimal experimental condition, which is 'fixation with 4% PFA, freezing with 10% DMSO, and storage at 80°C'. To evaluate long-term stability of QC materials prepared in this optimal condition, two levels of QC materials (QM1 and QM2) were thawed after 30, 33, 35, 37, 60, 62, 64, and 67 days of cryopreservation. Lymphocyte subset was analyzed with BD Multitest IMK kit (BD Biosciences, USA). QM1 and QM2 were stable after 1-2 months of cryopreservation (CV materials for lymphocyte subset analysis in resource-poor settings.

  15. Lymphocyte subset reference intervals in blood donors from northeastern Brazil



    Full Text Available The reference intervals for leukocytes and lymphocytes currently used by most clinical laboratories present limitations as they are primarily derived from individuals of North American and European origin. The objective this study was to determine reference values for peripheral blood B lymphocytes, T lymphocyte subsets (CD4+, CD8+, naïve, memory, regulatory, TCRαβ and TCRγδ+ and NK cells from blood donors in Salvador-Bahia, Brazil. Results: The proportion of included male subjects was 73.7% and the median ages of males (34 and females (35 were found to be similar. Absolute counts total lymphocytes subsets to both gender was 1,956 (1,060-4,186 cells and relative values 34%. The T CD4+ and T CD8+ lymphocytes relative values was 51% (20-62 and 24% (9-28, respectively. The most statistically significant finding observed was a higher percentage of B lymphocytes (p=0.03 in females. Commonly cited subset reference intervals were found to be consistent with values in several populations from different geographic areas.

  16. Minimum Bayesian error probability-based gene subset selection.

    Li, Jian; Yu, Tian; Wei, Jin-Mao


    Sifting functional genes is crucial to the new strategies for drug discovery and prospective patient-tailored therapy. Generally, simply generating gene subset by selecting the top k individually superior genes may obtain an inferior gene combination, for some selected genes may be redundant with respect to some others. In this paper, we propose to select gene subset based on the criterion of minimum Bayesian error probability. The method dynamically evaluates all available genes and sifts only one gene at a time. A gene is selected if its combination with the other selected genes can gain better classification information. Within the generated gene subset, each individual gene is the most discriminative one in comparison with those that classify cancers in the same way as this gene does and different genes are more discriminative in combination than in individual. The genes selected in this way are likely to be functional ones from the system biology perspective, for genes tend to co-regulate rather than regulate individually. Experimental results show that the classifiers induced based on this method are capable of classifying cancers with high accuracy, while only a small number of genes are involved.

  17. A Dexterous Optional Randomized Response Model

    Tarray, Tanveer A.; Singh, Housila P.; Yan, Zaizai


    This article addresses the problem of estimating the proportion Pi[subscript S] of the population belonging to a sensitive group using optional randomized response technique in stratified sampling based on Mangat model that has proportional and Neyman allocation and larger gain in efficiency. Numerically, it is found that the suggested model is…

  18. Characterization of a chromosome-specific chimpanzee alpha satellite subset: Evolutionary relationship to subsets on human chromosomes

    Warburton, P.E.; Gosden, J.; Lawson, D. [Western General Hospital, Edinburgh (United Kingdom)] [and others


    Alpha satellite DNA is a tandemly repeated DNA family found at the centromeres of all primate chromosomes examined. The fundamental repeat units of alpha satellite DNA are diverged 169- to 172-bp monomers, often found to be organized in chromosome-specific higher-order repeat units. The chromosomes of human (Homo sapiens (HSA)), chimpanzee (Pan troglodytes (PTR) and Pan paniscus), and gorilla (Gorilla gorilla) share a remarkable similarity and synteny. It is of interest to ask if alpha satellite arrays at centromeres of homologous chromosomes between these species are closely related (evolving in an orthologous manner) or if the evolutionary processes that homogenize and spread these arrays within and between chromosomes result in nonorthologous evolution of arrays. By using PCR primers specific for human chromosome 17-specific alpha satellite DNA, we have amplified, cloned, and characterized a chromosome-specific subset from the PTR chimpanzee genome. Hybridization both on Southern blots and in situ as well as sequence analysis show that this subset is most closely related, as expected, to sequences on HSA 17. However, in situ hybridization reveals that this subset is not found on the homologous chromosome in chimpanzee (PTR 19), but instead on PTR 12, which is homologous to HSA 2p. 40 refs., 3 figs.

  19. Effect of immunoglobulin on T cell subsets and inflammatory cytokines of patients with viral meningoencephalitis

    Zhi-Cheng Bao; Xue-Long Xia; Ya-Ping Wu; Dan Liu


    Objective:To observe the effect of immunoglobulin in the adjuvant therapy of viral meningoencephalitis (VM).Methods: A total of 78 cases of VN in our hospital from September 2011 to April 2016 were chosen. According to the admission order, they were randomly divided into two groups, the control group and observation group, and 39 cases in each group. The control group was treated with acyclovir conventional therapy, and the observation group was supplemented by immunoglobulins. The differences of serum and cerebrospinal fluid levels of T cell subsets and inflammatory cytokines between the two groups before and after treatment were compared.Results:The total effective rate of the observation group was significantly higher than that in the control group (P<0.05). The serum and cerebrospinal fluid levels of T cell subsets CD3+, CD4+ and CD4+/CD8+ were significantly higher than those in the control group, while the CD8+ was lower than that in the control group (P<0.05). The serum and cerebrospinal fluid levels of inflammatory cytokines PCT, INF-γ, IL-6 and TNF-α were all significantly lower than those in the control group (P<0.05).Conclusions:Immunoglobulin can effectively improve the level of CD3+, CD4+ and VM in CD8 patients, and adjust the CD4/CD8 balance, inhibit synthesis and secretion of Th1cytokines, so to play the assistant effects of acyclovir in the treatment of VM.

  20. Chemical library subset selection algorithms: a unified derivation using spatial statistics.

    Hamprecht, Fred A; Thiel, Walter; van Gunsteren, Wilfred F


    If similar compounds have similar activity, rational subset selection becomes superior to random selection in screening for pharmacological lead discovery programs. Traditional approaches to this experimental design problem fall into two classes: (i) a linear or quadratic response function is assumed (ii) some space filling criterion is optimized. The assumptions underlying the first approach are clear but not always defendable; the second approach yields more intuitive designs but lacks a clear theoretical foundation. We model activity in a bioassay as realization of a stochastic process and use the best linear unbiased estimator to construct spatial sampling designs that optimize the integrated mean square prediction error, the maximum mean square prediction error, or the entropy. We argue that our approach constitutes a unifying framework encompassing most proposed techniques as limiting cases and sheds light on their underlying assumptions. In particular, vector quantization is obtained, in dimensions up to eight, in the limiting case of very smooth response surfaces for the integrated mean square error criterion. Closest packing is obtained for very rough surfaces under the integrated mean square error and entropy criteria. We suggest to use either the integrated mean square prediction error or the entropy as optimization criteria rather than approximations thereof and propose a scheme for direct iterative minimization of the integrated mean square prediction error. Finally, we discuss how the quality of chemical descriptors manifests itself and clarify the assumptions underlying the selection of diverse or representative subsets.

  1. Stratified flows with variable density: mathematical modelling and numerical challenges.

    Murillo, Javier; Navas-Montilla, Adrian


    Stratified flows appear in a wide variety of fundamental problems in hydrological and geophysical sciences. They may involve from hyperconcentrated floods carrying sediment causing collapse, landslides and debris flows, to suspended material in turbidity currents where turbulence is a key process. Also, in stratified flows variable horizontal density is present. Depending on the case, density varies according to the volumetric concentration of different components or species that can represent transported or suspended materials or soluble substances. Multilayer approaches based on the shallow water equations provide suitable models but are not free from difficulties when moving to the numerical resolution of the governing equations. Considering the variety of temporal and spatial scales, transfer of mass and energy among layers may strongly differ from one case to another. As a consequence, in order to provide accurate solutions, very high order methods of proved quality are demanded. Under these complex scenarios it is necessary to observe that the numerical solution provides the expected order of accuracy but also converges to the physically based solution, which is not an easy task. To this purpose, this work will focus in the use of Energy balanced augmented solvers, in particular, the Augmented Roe Flux ADER scheme. References: J. Murillo , P. García-Navarro, Wave Riemann description of friction terms in unsteady shallow flows: Application to water and mud/debris floods. J. Comput. Phys. 231 (2012) 1963-2001. J. Murillo B. Latorre, P. García-Navarro. A Riemann solver for unsteady computation of 2D shallow flows with variable density. J. Comput. Phys.231 (2012) 4775-4807. A. Navas-Montilla, J. Murillo, Energy balanced numerical schemes with very high order. The Augmented Roe Flux ADER scheme. Application to the shallow water equations, J. Comput. Phys. 290 (2015) 188-218. A. Navas-Montilla, J. Murillo, Asymptotically and exactly energy balanced augmented flux

  2. Deep silicon maxima in the stratified oligotrophic Mediterranean Sea

    Y. Crombet


    Full Text Available The silicon biogeochemical cycle has been studied in the Mediterranean Sea during late summer/early autumn 1999 and summer 2008. The distribution of nutrients, particulate carbon and silicon, fucoxanthin (Fuco, and total chlorophyll-a (TChl-a were investigated along an eastward gradient of oligotrophy during two cruises (PROSOPE and BOUM encompassing the entire Mediterranean Sea during the stratified period. At both seasons, surface waters were depleted in nutrients and the nutriclines gradually deepened towards the East, the phosphacline being the deepest in the easternmost Levantine basin. Following the nutriclines, parallel deep maxima of biogenic silica (DSM, fucoxanthin (DFM and TChl-a (DCM were evidenced during both seasons with maximal concentrations of 0.45 μmol L−1 for BSi, 0.26 μg L−1 for Fuco, and 1.70 μg L−1 for TChl-a, all measured during summer. Contrary to the DCM which was a persistent feature in the Mediterranean Sea, the DSM and DFMs were observed in discrete areas of the Alboran Sea, the Algero-Provencal basin, the Ionian sea and the Levantine basin, indicating that diatoms were able to grow at depth and dominate the DCM under specific conditions. Diatom assemblages were dominated by Chaetoceros spp., Leptocylindrus spp., Pseudonitzschia spp. and the association between large centric diatoms (Hemiaulus hauckii and Rhizosolenia styliformis and the cyanobacterium Richelia intracellularis was observed at nearly all sites. The diatom's ability to grow at depth is commonly observed in other oligotrophic regions and could play a major role in ecosystem productivity and carbon export to depth. Contrary to the common view that Si and siliceous phytoplankton are not major components of the Mediterranean biogeochemistry, we suggest here that diatoms, by persisting at depth during the stratified period, could contribute to a

  3. Fishing and the oceanography of a stratified shelf sea

    Sharples, Jonathan; Ellis, Jim R.; Nolan, Glenn; Scott, Beth E.


    Fishing vessel position data from the Vessel Monitoring System (VMS) were used to investigate fishing activity in the Celtic Sea, a seasonally-stratifying, temperate region on the shelf of northwest Europe. The spatial pattern of fishing showed that three main areas are targeted: (1) the Celtic Deep (an area of deeper water with fine sediments), (2) the shelf edge, and (3) an area covering several large seabed banks in the central Celtic Sea. Data from each of these regions were analysed to examine the contrasting seasonality of fishing activity, and to highlight where the spring-neap tidal cycle appears to be important to fishing. The oceanographic characteristics of the Celtic Sea were considered alongside the distribution and timing of fishing, illustrating likely contrasts in the underlying environmental drivers of the different fished regions. In the central Celtic Sea, fishing mainly occurred during the stratified period between April and August. Based on evidence provided in other papers of this Special Issue, we suggest that the fishing in this area is supported by (1) a broad increase in primary production caused by lee-waves generated by seabed banks around spring tides driving large supplies of nutrients into the photic zone, and (2) greater concentrations of zooplankton within the region influenced by the seabed banks and elevated primary production. In contrast, while the shelf edge is a site of elevated surface chlorophyll, previous work has suggested that the periodic mixing generated by an internal tide at the shelf edge alters the size-structure of the phytoplankton community which fish larvae from the spawning stocks along the shelf edge are able to exploit. The fishery for Nephrops norvegicus in the Celtic Deep was the only one to show a significant spring-neap cycle, possibly linked to Nephrops foraging outside their burrows less during spring tides. More tentatively, the fishery for Nephrops correlated most strongly with a localised shift in

  4. T lymphocyte subset imbalances in patients contribute to ankylosing spondylitis



    Ankylosing spondylitis is a chronic inflammatory rheumatic disease, which is characterized by inflammation of the spine and the sacroiliac joints. To date, the disease etiology remains unclear. In the present study, the correlation of T lymphocyte subset changes with the progression of ankylosing spondylitis was investigated. A total of 55 patients with ankylosing spondylitis (22 severe and 23 mild cases) and 20 healthy individuals were selected. Firstly, the punctured cells in the lesions and the serum were collected, and the lymphocytes and the peripheral blood mononuclear cells were prepared. Secondly, quantitative PCR, ELISA and flow cytometry analyses were carried out to detect the levels of a series of immunoglobulins, complements, helper T cells, cytotoxic T cells, regulatory cells and cytokines. The expression levels of α-globulin, γ-globulin, immunoglobulin (Ig)G, IgA, IgM, serum complement C3, and complement C4 were found to be significantly increased in ankylosing spondylitis patients. In addition, the percentage of Th1 and Th17 cells was found to be significantly higher in the ankylosing spondylitis groups (mild and severe) compared with the healthy individuals. As a result, the Th1/Th2 and Th17/Treg ratios were significantly higher in patients with ankylosing spondylitis. In addition, T lymphocyte subset ratio imbalances contributed to an increased expression of immune mediators, including interferon (IFN)-γ and interleukin (IL)-17A. The mRNA and protein expression levels of IFN-γ and IL-17A were found to be higher in the ankylosing spondylitis groups compared with the control group. The present study provided further evidence on the function and underlying mechanism of T lymphocyte subsets, which may be useful in the diagnosis and treatment of ankylosing spondylitis. PMID:25452811

  5. T lymphocyte subset imbalances in patients contribute to ankylosing spondylitis.

    Wang, Chenggong; Liao, Qiande; Hu, Yihe; Zhong, DA


    Ankylosing spondylitis is a chronic inflammatory rheumatic disease, which is characterized by inflammation of the spine and the sacroiliac joints. To date, the disease etiology remains unclear. In the present study, the correlation of T lymphocyte subset changes with the progression of ankylosing spondylitis was investigated. A total of 55 patients with ankylosing spondylitis (22 severe and 23 mild cases) and 20 healthy individuals were selected. Firstly, the punctured cells in the lesions and the serum were collected, and the lymphocytes and the peripheral blood mononuclear cells were prepared. Secondly, quantitative PCR, ELISA and flow cytometry analyses were carried out to detect the levels of a series of immunoglobulins, complements, helper T cells, cytotoxic T cells, regulatory cells and cytokines. The expression levels of α-globulin, γ-globulin, immunoglobulin (Ig)G, IgA, IgM, serum complement C3, and complement C4 were found to be significantly increased in ankylosing spondylitis patients. In addition, the percentage of Th1 and Th17 cells was found to be significantly higher in the ankylosing spondylitis groups (mild and severe) compared with the healthy individuals. As a result, the Th1/Th2 and Th17/Treg ratios were significantly higher in patients with ankylosing spondylitis. In addition, T lymphocyte subset ratio imbalances contributed to an increased expression of immune mediators, including interferon (IFN)-γ and interleukin (IL)-17A. The mRNA and protein expression levels of IFN-γ and IL-17A were found to be higher in the ankylosing spondylitis groups compared with the control group. The present study provided further evidence on the function and underlying mechanism of T lymphocyte subsets, which may be useful in the diagnosis and treatment of ankylosing spondylitis.

  6. Cytokine profile and lymphocyte subsets in type 2 diabetes

    C.O. Francisco


    Full Text Available Type 2 diabetes mellitus (T2D is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old with T2D and 20 nonsmoking men (49.4±7.6 years old who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were measured by a commercially available automated system. Frequencies of lymphocyte subsets in peripheral blood and intracellular production of interleukin (IL-4, IL-10, IL-17, tumor necrosis factor-α, and interferon-γ cytokines by CD3+ T cells were assessed by flow cytometry. No differences were observed in the frequency of CD19+ B cells, CD3+CD8+ and CD3+CD4+ T cells, CD16+56+ NK cells, and CD4+CD25+Foxp3+ T regulatory cells in patients with T2D compared with controls. The numbers of IL-10- and IL-17-producing CD3+ T cells were significantly higher in patients with T2D than in controls (P<0.05. The frequency of interferon-γ-producing CD3+ T cells was positively correlated with body mass index (r=0.59; P=0.01. In conclusion, this study shows increased numbers of circulating IL-10- and IL-17-producing CD3+ T cells in patients with T2D, suggesting that these cytokines are involved in the immune pathology of this disease.

  7. Profiling leucocyte subsets in tuberculosis-diabetes co-morbidity.

    Kumar, Nathella Pavan; Moideen, Kadar; Dhakshinraj, Sharmila D; Banurekha, Vaithilingam V; Nair, Dina; Dolla, Chandrakumar; Kumaran, Paul; Babu, Subash


    The immune system plays an important role in the pathogenesis of pulmonary tuberculosis-type 2 diabetes mellitus (PTB-DM) co-morbidity. However, the phenotypic profile of leucocyte subsets at homeostasis in individuals with active or latent tuberculosis (LTB) with coincident diabetes is not known. To characterize the influence of diabetes on leucocyte phenotypes in PTB or LTB, we examined the frequency (Fo ) of leucocyte subsets in individuals with TB with (PTB-DM) or without (PTB) diabetes; individuals with latent TB with (LTB-DM) or without (LTB) diabetes and non-TB-infected individuals with (NTB-DM) or without (NTB) diabetes. Coincident DM is characterized by significantly lower Fo of effector memory CD4(+) T cells in LTB individuals. In contrast, DM is characterized by significantly lower Fo of effector memory CD8(+) T cells and significantly higher Fo of central memory CD8(+) T cells in PTB individuals. Coincident DM resulted in significantly higher Fo of classical memory B cells in PTB and significantly higher Fo of activated memory and atypical B cells in LTB individuals. Coincident DM resulted in significantly lower Fo of classical and intermediate monocytes in PTB, LTB and NTB individuals. Finally, DM resulted in significantly lower Fo of myeloid and plasmacytoid dendritic cells in PTB, LTB and NTB individuals. Our data reveal that coincident diabetes alters the cellular subset distribution of T cells, B cells, dendritic cells and monocytes in both individuals with active TB and those with latent TB, thus potentially impacting the pathogenesis of this co-morbid condition.

  8. On filling families of finite subsets of the Cantor set

    Dodos, Pandelis


    Let $\\ee>0$ and $\\fff$ be a family of finite subsets of the Cantor set $\\ccc$. Following D. H. Fremlin, we say that $\\fff$ is $\\ee$-filling over $\\ccc$ if $\\fff$ is hereditary and for every $F\\subseteq\\ccc$ finite there exists $G\\subseteq F$ such that $G\\in\\fff$ and $|G|\\geq\\ee |F|$. We show that if $\\fff$ is $\\ee$-filling over $\\ccc$ and $C$-measurable in $[\\ccc]^{<\\omega}$, then for every $P\\subseteq\\ccc$ perfect there exists $Q\\subseteq P$ perfect with $[Q]^{<\\omega}\\subseteq\\fff$. A similar result for weaker versions of density is also obtained.

  9. Cytokine-producing T cell subsets in human leishmaniasis

    Kemp, Kåre


    Leishmania specific Th1/Th2 cells have been identified in humans as well as in mice. There is a correlation between the clinical outcome of the infection and the cytokine response profile. Generally, the production of Th2 cytokines leads to severe infection, whereas the production of Th1 cytokine...... cells mutually down-regulate each other. However, the presence of antigen specific regulatory T cell subsets may provide an environment that allows the presence of both Th1 and Th2 cells....

  10. A novel cell subset:Interferon-producing killer dendritic cells


    Recent reports introduce a novel cell subset of DCs with antigenic phenotypes shared by both NK cells and B cells, but without surface markers of pDCs and T cells, appearing to be a chimera of NK cells and DCs, namely interferon-producing killer dendritic cells(IKDCs).IKDCs not only secret type I and type II interferons to recognize and kill tumor cells effectively, but also express MHC-II molecules to present antigens.Thus, IKDCs are considered as important immunosurveilance cells for tumors, providing a link between innate and adaptive immunity.

  11. Feature Subset Selection by Estimation of Distribution Algorithms

    Cantu-Paz, E


    This paper describes the application of four evolutionary algorithms to the identification of feature subsets for classification problems. Besides a simple GA, the paper considers three estimation of distribution algorithms (EDAs): a compact GA, an extended compact GA, and the Bayesian Optimization Algorithm. The objective is to determine if the EDAs present advantages over the simple GA in terms of accuracy or speed in this problem. The experiments used a Naive Bayes classifier and public-domain and artificial data sets. In contrast with previous studies, we did not find evidence to support or reject the use of EDAs for this problem.

  12. Immunity to pathogens taught by specialized human dendritic cell subsets.

    Jens A. E. Geginat


    Full Text Available Dendritic cells (DC are specialized antigen-presenting cells (APC that have a key role in immune responses, because they bridge the innate and adaptive arms of the immune system. They mature upon recognition of pathogens and up-regulate MHC molecules and co-stimulatory receptors to activate antigen-specific CD4+ and CD8+ T-cells. It is now well established that DC are not a homogeneous population, but are composed of different subsets with specialized functions in immune responses to specific pathogens. Upon viral infections, plasmacytoid DC (pDC rapidly produce large amounts of IFN-α, which has potent anti-viral functions and activates several other immune cells. However, pDC are not particularly potent APC and induce the tolerogenic cytokine IL-10 in CD4+ T-cells. In contrast, myeloid DC (mDC are very potent APC and possess the unique capacity to prime naïve T-cells and consequently to initiate a primary adaptive immune response. Different subsets of myeloid DC with specialized functions have been identified. In mice, CD8α+ mDC capture antigenic material from necrotic cells, secrete high levels of IL-12, and prime Th1 and cytotoxic T cell responses to control intracellular pathogens. Conversely, CD8α- mDC preferentially prime CD4+ T-cells and promote Th2 or Th17 differentiation. BDCA-3+ mDC2 are the human homologue of CD8α+ mDC, since they share the expression of several key molecules, the capacity to cross-present antigens to CD8+ T-cells and to produce IFN-λ. However, although several features of the DC network are conserved between humans and mice, the expression of several relevant toll-like receptors as well as the production of cytokines that regulate T-cell differentiation are different. Intriguingly, recent data suggests specific roles for human DC subsets in immune responses against individual pathogens. The biology of human DC subsets holds the promise to be exploitable in translational medicine, in particular for the

  13. A maximum feasible subset algorithm with application to radiation therapy

    Sadegh, Payman


    inequalities. Special classes of this problem are of interest in a variety of areas such as pattern recognition, machine learning, operations research, and medical treatment planning. This problem is generally solvable in exponential time. A heuristic polynomial time algorithm is presented in this paper......Consider a set of linear one sided or two sided inequality constraints on a real vector X. The problem of interest is selection of X so as to maximize the number of constraints that are simultaneously satisfied, or equivalently, combinatorial selection of a maximum cardinality subset of feasible...

  14. Individual discriminative face recognition models based on subsets of features

    Clemmensen, Line Katrine Harder; Gomez, David Delgado; Ersbøll, Bjarne Kjær


    of the face recognition problem. The elastic net model is able to select a subset of features with low computational effort compared to other state-of-the-art feature selection methods. Furthermore, the fact that the number of features usually is larger than the number of images in the data base makes feature...... selection techniques such as forward selection or lasso regression become inadequate. In the experimental section, the performance of the elastic net model is compared with geometrical and color based algorithms widely used in face recognition such as Procrustes nearest neighbor, Eigenfaces, or Fisher...

  15. Macrophage subset sensitivity to endotoxin tolerisation by Porphyromonas gingivalis.

    Andrew D Foey

    Full Text Available Macrophages (MΦs determine oral mucosal responses; mediating tolerance to commensal microbes and food whilst maintaining the capacity to activate immune defences to pathogens. MΦ responses are determined by both differentiation and activation stimuli, giving rise to two distinct subsets; pro-inflammatory M1- and anti-inflammatory/regulatory M2- MΦs. M2-like subsets predominate tolerance induction whereas M1 MΦs predominate in inflammatory pathologies, mediating destructive inflammatory mechanisms, such as those in chronic P.gingivalis (PG periodontal infection. MΦ responses can be suppressed to benefit either the host or the pathogen. Chronic stimulation by bacterial pathogen associated molecular patterns (PAMPs, such as LPS, is well established to induce tolerance. The aim of this study was to investigate the susceptibility of MΦ subsets to suppression by P. gingivalis. CD14(hi and CD14(lo M1- and M2-like MΦs were generated in vitro from the THP-1 monocyte cell line by differentiation with PMA and vitamin D3, respectively. MΦ subsets were pre-treated with heat-killed PG (HKPG and PG-LPS prior to stimulation by bacterial PAMPs. Modulation of inflammation was measured by TNFα, IL-1β, IL-6, IL-10 ELISA and NFκB activation by reporter gene assay. HKPG and PG-LPS differentially suppress PAMP-induced TNFα, IL-6 and IL-10 but fail to suppress IL-1β expression in M1 and M2 MΦs. In addition, P.gingivalis suppressed NFκB activation in CD14(lo and CD14(hi M2 regulatory MΦs and CD14(lo M1 MΦs whereas CD14(hi M1 pro-inflammatory MΦs were refractory to suppression. In conclusion, P.gingivalis selectively tolerises regulatory M2 MΦs with little effect on pro-inflammatory CD14(hi M1 MΦs; differential suppression facilitating immunopathology at the expense of immunity.

  16. Randomization tests

    Edgington, Eugene


    Statistical Tests That Do Not Require Random Sampling Randomization Tests Numerical Examples Randomization Tests and Nonrandom Samples The Prevalence of Nonrandom Samples in Experiments The Irrelevance of Random Samples for the Typical Experiment Generalizing from Nonrandom Samples Intelligibility Respect for the Validity of Randomization Tests Versatility Practicality Precursors of Randomization Tests Other Applications of Permutation Tests Questions and Exercises Notes References Randomized Experiments Unique Benefits of Experiments Experimentation without Mani

  17. Turbulence comes in bursts in stably stratified flows

    Rorai, C; Pouquet, A


    There is a clear distinction between simple laminar and complex turbulent fluids. But in some cases, as for the nocturnal planetary boundary layer, a stable and well-ordered flow can develop intense and sporadic bursts of turbulent activity which disappear slowly in time. This phenomenon is ill-understood and poorly modeled; and yet, it is central to our understanding of weather and climate dynamics. We present here a simple model which shows that in stably stratified turbulence, the stronger bursts can occur when the flow is expected to be more stable. The bursts are generated by a rapid non-linear amplification of energy stored in waves, and are associated with energetic interchanges between vertical velocity and temperature (or density) fluctuations. Direct numerical simulations on grids of 2048^3 points confirm this somewhat paradoxical result of measurably stronger events for more stable flows, displayed not only in the temperature and vertical velocity derivatives, but also in the amplitude of the field...

  18. DNS of stratified spatially-developing turbulent thermal boundary layers

    Araya, Guillermo; Castillo, Luciano; Jansen, Kenneth


    Direct numerical simulations (DNS) of spatially-developing turbulent thermal boundary layers under stratification are performed. It is well known that the transport phenomena of the flow is significantly affected by buoyancy, particularly in urban environments where stable and unstable atmospheric boundary layers are encountered. In the present investigation, the Dynamic Multi-scale approach by Araya et al. (JFM, 670, 2011) for turbulent inflow generation is extended to thermally stratified boundary layers. Furthermore, the proposed Dynamic Multi-scale approach is based on the original rescaling-recycling method by Lund et al. (1998). The two major improvements are: (i) the utilization of two different scaling laws in the inner and outer parts of the boundary layer to better absorb external conditions such as inlet Reynolds numbers, streamwise pressure gradients, buoyancy effects, etc., (ii) the implementation of a Dynamic approach to compute scaling parameters from the flow solution without the need of empirical correlations as in Lund et al. (1998). Numerical results are shown for ZPG flows at high momentum thickness Reynolds numbers (~ 3,000) and a comparison with experimental data is also carried out.

  19. Stratified patterns of divorce: Earnings, education, and gender

    Amit Kaplan


    Full Text Available Background: Despite evidence that divorce has become more prevalent among weaker socioeconomic groups, knowledge about the stratification aspects of divorce in Israel is lacking. Moreover, although scholarly debate recognizes the importance of stratificational positions with respect to divorce, less attention has been given to the interactions between them. Objective: Our aim is to examine the relationship between social inequality and divorce, focusing on how household income, education, employment stability, relative earnings, and the intersection between them affect the risk of divorce in Israel. Methods: The data is derived from combined census files for 1995-2008, annual administrative employment records from the National Insurance Institute and the Tax Authority, and data from the Civil Registry of Divorce. We used a series of discrete-time event-history analysis models for marital dissolution. Results: Couples in lower socioeconomic positions had a higher risk of divorce in Israel. Higher education in general, and homogamy in terms of higher education (both spouses have degrees in particular, decreased the risk of divorce. The wife's relative earnings had a differential effect on the likelihood of divorce, depending on household income: a wife who outearned her husband increased the log odds of divorce more in the upper tertiles than in the lower tertile. Conclusions: Our study shows that divorce indeed has a stratified pattern and that weaker socioeconomic groups experience the highest levels of divorce. Gender inequality within couples intersects with the household's economic and educational resources.

  20. Self-Knowledge and Risk in Stratified Medicine.

    Hordern, Joshua


    This article considers why and how self-knowledge is important to communication about risk and behaviour change by arguing for four claims. First, it is doubtful that genetic knowledge should properly be called 'self-knowledge' when its ordinary effects on self-motivation and behaviour change seem so slight. Second, temptations towards a reductionist, fatalist, construal of persons' futures through a 'molecular optic' should be resisted. Third, any plausible effort to change people's behaviour must engage with cultural self-knowledge, values and beliefs, catalysed by the communication of genetic risk. For example, while a Judaeo-Christian notion of self-knowledge is distinctively theological, people's self-knowledge is plural in its insight and sources. Fourth, self-knowledge is found in compassionate, if tense, communion which yields freedom from determinism even amidst suffering. Stratified medicine thus offers a newly precise kind of humanising health care through societal solidarity with the riskiest. However, stratification may also mean that molecularly unstratified, 'B' patients' experience involves accentuated suffering and disappointment, a concern requiring further research.

  1. [Phylogenetic diversity of bacteria in soda lake stratified sediments].

    Tourova, T P; Grechnikova, M A; Kuznetsov, V V; Sorokin, D Yu


    Various previously developed techniques for DNA extraction from the samples with complex physicochemical structure (soils, silts, and sediments) and modifications of these techniques developed in the present work were tested. Their usability for DNA extraction from the sediments of the Kulunda Steppe hypersaline soda lakes was assessed, and the most efficient procedure for indirect (two-stage) DNA extraction was proposed. Almost complete separation of the cell fraction was shown, as well as the inefficiency of nested PCR for analysis of the clone libraries obtained from washed sediments by amplification of the 16S rRNA gene fragments. Analysis of the clone library obtained from the cell fractions of stratified sediments (upper, medium, and lower layers) revealed that in the sediments of Lake Gorchina-3 most eubacterial phylotypes belonged to the class Clostridia, phylum Firmicutes. They were probably specific for this habitatand formed a new, presently unknown high-rank taxon. The data obtained revealed no pronounced stratification of the spe- cies diversity of the eubacterial component of the microbial community inhabiting the sediments (0-20 cm) in the inshore zone of Lake Gorchina-3.

  2. Stratified Flow Past a Hill: Dividing Streamline Concept Revisited

    Leo, Laura S.; Thompson, Michael Y.; Di Sabatino, Silvana; Fernando, Harindra J. S.


    The Sheppard formula (Q J R Meteorol Soc 82:528-529, 1956) for the dividing streamline height H_s assumes a uniform velocity U_∞ and a constant buoyancy frequency N for the approach flow towards a mountain of height h, and takes the form H_s/h=( {1-F} ) , where F=U_{∞}/Nh. We extend this solution to a logarithmic approach-velocity profile with constant N. An analytical solution is obtained for H_s/h in terms of Lambert-W functions, which also suggests alternative scaling for H_s/h. A `modified' logarithmic velocity profile is proposed for stably stratified atmospheric boundary-layer flows. A field experiment designed to observe H_s is described, which utilized instrumentation from the spring field campaign of the Mountain Terrain Atmospheric Modeling and Observations (MATERHORN) Program. Multiple releases of smoke at F≈ 0.3-0.4 support the new formulation, notwithstanding the limited success of experiments due to logistical constraints. No dividing streamline is discerned for F≈ 10, since, if present, it is too close to the foothill. Flow separation and vortex shedding is observed in this case. The proposed modified logarithmic profile is in reasonable agreement with experimental observations.

  3. Large eddy simulation of unsteady lean stratified premixed combustion

    Duwig, C. [Division of Fluid Mechanics, Department of Energy Sciences, Lund University, SE 221 00 Lund (Sweden); Fureby, C. [Division of Weapons and Protection, Warheads and Propulsion, The Swedish Defense Research Agency, FOI, SE 147 25 Tumba (Sweden)


    Premixed turbulent flame-based technologies are rapidly growing in importance, with applications to modern clean combustion devices for both power generation and aeropropulsion. However, the gain in decreasing harmful emissions might be canceled by rising combustion instabilities. Unwanted unsteady flame phenomena that might even destroy the whole device have been widely reported and are subject to intensive studies. In the present paper, we use unsteady numerical tools for simulating an unsteady and well-documented flame. Computations were performed for nonreacting, perfectly premixed and stratified premixed cases using two different numerical codes and different large-eddy-simulation-based flamelet models. Nonreacting simulations are shown to agree well with experimental data, with the LES results capturing the mean features (symmetry breaking) as well as the fluctuation level of the turbulent flow. For reacting cases, the uncertainty induced by the time-averaging technique limited the comparisons. Given an estimate of the uncertainty, the numerical results were found to reproduce well the experimental data in terms both of mean flow field and of fluctuation levels. In addition, it was found that despite relying on different assumptions/simplifications, both numerical tools lead to similar predictions, giving confidence in the results. Moreover, we studied the flame dynamics and particularly the response to a periodic pulsation. We found that above a certain excitation level, the flame dynamic changes and becomes rather insensitive to the excitation/instability amplitude. Conclusions regarding the self-growth of thermoacoustic waves were drawn. (author)

  4. Economic evaluation in stratified medicine: methodological issues and challenges

    Hans-Joerg eFugel


    Full Text Available Background: Stratified Medicine (SM is becoming a practical reality with the targeting of medicines by using a biomarker or genetic-based diagnostic to identify the eligible patient sub-population. Like any healthcare intervention, SM interventions have costs and consequences that must be considered by reimbursement authorities with limited resources. Methodological standards and guidelines exist for economic evaluations in clinical pharmacology and are an important component for health technology assessments (HTAs in many countries. However, these guidelines have initially been developed for traditional pharmaceuticals and not for complex interventions with multiple components. This raises the issue as to whether these guidelines are adequate to SM interventions or whether new specific guidance and methodology is needed to avoid inconsistencies and contradictory findings when assessing economic value in SM.Objective: This article describes specific methodological challenges when conducting health economic (HE evaluations for SM interventions and outlines potential modifications necessary to existing evaluation guidelines /principles that would promote consistent economic evaluations for SM.Results/Conclusions: Specific methodological aspects for SM comprise considerations on the choice of comparator, measuring effectiveness and outcomes, appropriate modelling structure and the scope of sensitivity analyses. Although current HE methodology can be applied for SM, greater complexity requires further methodology development and modifications in the guidelines.


    Jabbari, Sarah; Brandenburg, Axel; Kleeorin, Nathan; Mitra, Dhrubaditya; Rogachevskii, Igor, E-mail: [Nordita, KTH Royal Institute of Technology and Stockholm University, Roslagstullsbacken 23, SE-10691 Stockholm (Sweden)


    Recent work by Mitra et al. (2014) has shown that in strongly stratified forced two-layer turbulence with helicity and corresponding large-scale dynamo action in the lower layer, and nonhelical turbulence in the upper, a magnetic field occurs in the upper layer in the form of sharply bounded bipolar magnetic spots. Here we extend this model to spherical wedge geometry covering the northern hemisphere up to 75° latitude and an azimuthal extent of 180°. The kinetic helicity and therefore also the large-scale magnetic field are strongest at low latitudes. For moderately strong stratification, several bipolar spots form that eventually fill the full longitudinal extent. At early times, the polarity of spots reflects the orientation of the underlying azimuthal field, as expected from Parker’s Ω-shaped flux loops. At late times their tilt changes such that there is a radial field of opposite orientation at different latitudes separated by about 10°. Our model demonstrates the spontaneous formation of spots of sizes much larger than the pressure scale height. Their tendency to produce filling factors close to unity is argued to be reminiscent of highly active stars. We confirm that strong stratification and strong scale separation are essential ingredients behind magnetic spot formation, which appears to be associated with downflows at larger depths.

  6. Local properties of countercurrent stratified steam-water flow

    Kim, H J


    A study of steam condensation in countercurrent stratified flow of steam and subcooled water has been carried out in a rectangular channel/flat plate geometry over a wide range of inclination angles (4/sup 0/-87/sup 0/) at several aspect ratios. Variables were inlet water and steam flow rates, and inlet water temperature. Local condensation rates and pressure gradients were measured, and local condensation heat transfer coefficients and interfacial shear stress were calculated. Contact probe traverses of the surface waves were made, which allowed a statistical analysis of the wave properties. The local condensation Nusselt number was correlated in terms of local water and steam Reynolds or Froude numbers, as well as the liquid Prandtl number. A turbulence-centered model developed by Theofanous, et al. principally for gas absorption in several geometries, was modified. A correlation for the interfacial shear stress and the pressure gradient agreed with measured values. Mean water layer thicknesses were calculated. Interfacial wave parameters, such as the mean water layer thickness, liquid fraction probability distribution, wave amplitude and wave frequency, are analyzed.

  7. Numerical Study of Stratified Charge Combustion in Wave Rotors

    Nalim, M. Razi


    A wave rotor may be used as a pressure-gain combustor effecting non-steady flow, and intermittent, confined combustion to enhance gas turbine engine performance. It will be more compact and probably lighter than an equivalent pressure-exchange wave rotor, yet will have similar thermodynamic and mechanical characteristics. Because the allowable turbine blade temperature limits overall fuel/air ratio to sub-flammable values, premixed stratification techniques are necessary to burn hydrocarbon fuels in small engines with compressor discharge temperature well below autoignition conditions. One-dimensional, unsteady numerical simulations of stratified-charge combustion are performed using an eddy-diffusivity turbulence model and a simple reaction model incorporating a flammability limit temperature. For good combustion efficiency, a stratification strategy is developed which concentrates fuel at the leading and trailing edges of the inlet port. Rotor and exhaust temperature profiles and performance predictions are presented at three representative operating conditions of the engine: full design load, 40% load, and idle. The results indicate that peak local gas temperatures will result in excessive temperatures within the rotor housing unless additional cooling methods are used. The rotor itself will have acceptable temperatures, but the pattern factor presented to the turbine may be of concern, depending on exhaust duct design and duct-rotor interaction.

  8. Stratifying the Risk of Venous Thromboembolism in Otolaryngology

    Shuman, Andrew G.; Hu, Hsou Mei; Pannucci, Christopher J.; Jackson, Christopher R.; Bradford, Carol R.; Bahl, Vinita


    Objective The consequences of perioperative venous thromboembolism (VTE) are devastating; identifying patients at risk is an essential step in reducing morbidity and mortality. The utility of perioperative VTE risk assessment in otolaryngology is unknown. This study was designed to risk-stratify a diverse population of otolaryngology patients for VTE events. Study Design Retrospective cohort study. Setting Single-institution academic tertiary care medical center. Subjects and Methods Adult patients presenting for otolaryngologic surgery requiring hospital admission from 2003 to 2010 who did not receive VTE chemoprophylaxis were included. The Caprini risk assessment was retrospectively scored via a validated method of electronic chart abstraction. Primary study variables were Caprini risk scores and the incidence of perioperative venous thromboembolic outcomes. Results A total of 2016 patients were identified. The overall 30-day rate of VTE was 1.3%. The incidence of VTE in patients with a Caprini risk score of 6 or less was 0.5%. For patients with scores of 7 or 8, the incidence was 2.4%. Patients with a Caprini risk score greater than 8 had an 18.3% incidence of VTE and were significantly more likely to develop a VTE when compared to patients with a Caprini risk score less than 8 (P otolaryngology patients for 30-day VTE events and allows otolaryngologists to identify patient subgroups who have a higher risk of VTE in the absence of chemoprophylaxis. PMID:22261490

  9. Mixing efficiency of turbulent patches in stably stratified flows

    Garanaik, Amrapalli; Venayagamoorthy, Subhas Karan


    A key quantity that is essential for estimating the turbulent diapycnal (irreversible) mixing in stably stratified flow is the mixing efficiency Rf*, which is a measure of the amount of turbulent kinetic energy that is irreversibly converted into background potential energy. In particular, there is an ongoing debate in the oceanographic mixing community regarding the utility of the buoyancy Reynolds number (Reb) , particularly with regard to how mixing efficiency and diapycnal diffusivity vary with Reb . Specifically, is there a universal relationship between the intensity of turbulence and the strength of the stratification that supports an unambiguous description of mixing efficiency based on Reb ? The focus of the present study is to investigate the variability of Rf* by considering oceanic turbulence data obtained from microstructure profiles in conjunction with data from laboratory experiments and DNS. Field data analysis has done by identifying turbulent patches using Thorpe sorting method for potential density. The analysis clearly shows that high mixing efficiencies can persist at high buoyancy Reynolds numbers. This is contradiction to previous studies which predict that mixing efficiency should decrease universally for Reb greater than O (100) . Funded by NSF and ONR.

  10. Simulation and study of stratified flows around finite bodies

    Gushchin, V. A.; Matyushin, P. V.


    The flows past a sphere and a square cylinder of diameter d moving horizontally at the velocity U in a linearly density-stratified viscous incompressible fluid are studied. The flows are described by the Navier-Stokes equations in the Boussinesq approximation. Variations in the spatial vortex structure of the flows are analyzed in detail in a wide range of dimensionless parameters (such as the Reynolds number Re = Ud/ ν and the internal Froude number Fr = U/( Nd), where ν is the kinematic viscosity and N is the buoyancy frequency) by applying mathematical simulation (on supercomputers of Joint Supercomputer Center of the Russian Academy of Sciences) and three-dimensional flow visualization. At 0.005 < Fr < 100, the classification of flow regimes for the sphere (for 1 < Re < 500) and for the cylinder (for 1 < Re < 200) is improved. At Fr = 0 (i.e., at U = 0), the problem of diffusion-induced flow past a sphere leading to the formation of horizontal density layers near the sphere's upper and lower poles is considered. At Fr = 0.1 and Re = 50, the formation of a steady flow past a square cylinder with wavy hanging density layers in the wake is studied in detail.

  11. A random spatial sampling method in a rural developing nation

    Michelle C. Kondo; Kent D.W. Bream; Frances K. Barg; Charles C. Branas


    Nonrandom sampling of populations in developing nations has limitations and can inaccurately estimate health phenomena, especially among hard-to-reach populations such as rural residents. However, random sampling of rural populations in developing nations can be challenged by incomplete enumeration of the base population. We describe a stratified random sampling method...

  12. Peripheral blood and milk leukocytes subsets of lactating Sarda ewes

    Piero Bonelli


    Full Text Available Leukocytes subpopulations in blood and milk of lactating Sarda ewes were investigated. Animals characterized by a SSC level <500×103cells/mL and a negative bacteriological examination were sampled in early, mid and late lactation. Milk differential cell count evidenced that macrophage represented the main population (42.8%±3.5 followed by lymphocytes (40.2%±3.4 and neutrophils (8,6%±2.1. Flow cytometry analysis showed that lymphocytes subsets in milk were quite different from blood. High CD8+ and low CD4+ lymphocytes percentages determined a CD4/CD8 ratio inversion in milk compared to blood (0.3%±0.03 vs 1.8%±0.08. CD8+ decreased while, conversely, CD4+ increased in late lactation. γδ T cells were more represented in milk (12.6%±1.3 than in blood (6.8%±0.3 and their proportions appeared similar throughout lactation in both compartments. IL-2 receptor was mainly expressed in milk on T cytotoxic lymphocytes. Data obtained in uninfected mammary glands could allow an early discrimination between physiological and pathological changes occurring in ewe milk. Further phenotypical and functional studies on milk leukocytes subsets might help to understand defense mechanisms of the ovine mammary gland against IMI.

  13. A Hybrid Feature Subset Selection using Metrics and Forward Selection

    K. Fathima Bibi


    Full Text Available The aim of this study is to design a Feature Subset Selection Technique that speeds up the Feature Selection (FS process in high dimensional datasets with reduced computational cost and great efficiency. FS has become the focus of much research on decision support system areas for which data with tremendous number of variables are analyzed. Filters and wrappers are proposed techniques for the feature subset selection process. Filters make use of association based approach but wrappers adopt classification algorithms to identify important features. Filter method lacks the ability of minimization of simplification error while wrapper method burden weighty computational resource. To pull through these difficulties, a hybrid approach is proposed combining both filters and wrappers. Filter approach uses a permutation of ranker search methods and a wrapper which improves the learning accurateness and obtains a lessening in the memory requirements and finishing time. The UCI machine learning repository was chosen to experiment the approach. The classification accuracy resulted from our approach proves to be higher.

  14. A subset of interneurons required for Drosophila larval locomotion.

    Yoshikawa, Shingo; Long, Hong; Thomas, John B


    Efforts to define the neural circuits generating locomotor behavior have produced an initial understanding of some of the components within the spinal cord, as well as a basic understanding of several invertebrate motor pattern generators. However, how these circuits are assembled during development is poorly understood. We are defining the neural circuit that generates larval locomotion in the genetically tractable fruit fly Drosophila melanogaster to study locomotor circuit development. Forward larval locomotion involves a stereotyped posterior-to-anterior segmental translocation of body wall muscle contraction and is generated by a relatively small number of identified muscles, motor and sensory neurons, plus an unknown number of the ~270 bilaterally-paired interneurons per segment of the 1st instar larva. To begin identifying the relevant interneurons, we have conditionally inactivated synaptic transmission of interneuron subsets and assayed for the effects on locomotion. From this screen we have identified a subset of 25 interneurons per hemisegment, called the lateral locomotor neurons (LLNs), that are required for locomotion. Both inactivation and constitutive activation of the LLNs disrupt locomotion, indicating that patterned output of the LLNs is required. By expressing a calcium indicator in the LLNs, we found that they display a posterior-to-anterior wave of activity within the CNS corresponding to the segmental translocation of the muscle contraction wave. Identification of the LLNs represents the first step toward elucidating the circuit generating larval locomotion.

  15. Niacin-respondent subset of schizophrenia – a therapeutic review.

    Xu, X J; Jiang, G S


    It is well known that niacin deficiency manifests with several psychiatric manifestations. Also historically evidence has accumulated that niacin augmentation can be used for treatment of schizophrenia. However, the etiopathological associations between niacin deficiency and schizophrenia as well as the mechanism of action of niacin in its treatment. More importantly, the subgroups of schizophrenia which will respond to niacin augmentation has never been highlighted in the literature. In this article, we review three of the mechanisms in which niacin deficiency could lead to schizophrenic symptoms: (1) Niacin deficiency neurodegeneration (2) Membrane phospholipid deficiency hypothesis and (3) Adrenochrome hypothesis. We will further move towards the clinical as well as treatment related associations as reviewed from the literature. Here, we propose a model that a subset of schizophrenia can respond to niacin augmentation therapy better than other subsets because these patients have contributions in their psychotic manifestations from the neural degeneration resulting from niacin deficiency. We present a short description of our case report which showed rapid improvement in schizophrenic psychotic symptoms subsequent to administration of niacin as an augmentation therapy. We, thus, propose that niacin deficiency is a contributory factor in schizophrenia development in some patients and symptom alleviation in these patients will benefit from niacin augmentation, especially in some particular psychotic features.

  16. Endothelial progenitor cell subsets and preeclampsia: Findings and controversies

    Armin Attar


    Full Text Available Vascular remodeling is an essential component of gestation. Endothelial progenitor cells (EPCs play an important role in the regulation of vascular homeostasis. The results of studies measuring the number of EPCs in normal pregnancies and in preeclampsia have been highly controversial or even contradictory because of some variations in technical issues and different methodologies enumerating three distinct subsets of EPCs: circulating angiogenic cells (CAC, colony forming unit endothelial cells (CFU-ECs, and endothelial colony-forming cells (ECFCs. In general, most studies have shown an increase in the number of CACs in the maternal circulation with a progression in the gestational age in normal pregnancies, while functional capacities measured by CFU-ECs and ECFCs remain intact. In the case of preeclampsia, mobilization of CACs and ECFCs occurs in the peripheral blood of pregnant women, but the functional capacities shown by culture of the derived colony-forming assays (CFU-EC and ECFC assays are altered. Furthermore, the number of all EPC subsets will be reduced in umbilical cord blood in the case of preeclampsia. As EPCs play an important role in the homeostasis of vascular networks, the difference in their frequency and functionality in normal pregnancies and those with preeclampsia can be expected. In this review, there was an attempt to provide a justification for these controversies.

  17. T-cell subsets in the germinal center.

    Ramiscal, Roybel R; Vinuesa, Carola G


    T cells are known to migrate to B-cell-enriched follicles and germinal centers within secondary lymphoid organs to provide help to B cells. Cognate T:B interactions that take place at the T:B border and subsequently within germinal centers are essential for B-cell priming, differentiation into germinal center B cells, and selection of mutated cells into memory B cells or memory plasma cells. In recent years, different stages of maturation within B-cell helper T cells, collectively known as B-follicular helper T (Tfh) cells, as well as heterogeneity amid germinal center T cells are becoming clear. Indeed, germinal centers support not only bona fide Tfh cells but also CD4(+) and CD8(+) follicular regulatory T (Tfr) cells that act to suppress germinal center responses and B-cell helper natural killer T cells. There is a growing need for more precise phenotypic and functional distinction of these specialized T-cell subsets. In this review, we summarize current knowledge on the ontogeny, molecular identity, and functional relevance of the various subsets of germinal center T cells.

  18. Monocyte Subsets in Schistosomiasis Patients with Periportal Fibrosis

    Jamille Souza Fernandes


    Full Text Available A major issue with Schistosoma mansoni infection is the development of periportal fibrosis, which is predominantly caused by the host immune response to egg antigens. Experimental studies have pointed to the participation of monocytes in the pathogenesis of liver fibrosis. The aim of this study was to characterize the subsets of monocytes in individuals with different degrees of periportal fibrosis secondary to schistosomiasis. Monocytes were classified into classical (CD14++CD16−, intermediate (CD14++CD16+, and nonclassical (CD14+CD16++. The expressions of monocyte markers and cytokines were assessed using flow cytometry. The frequency of classical monocytes was higher than the other subsets. The expression of HLA-DR, IL-6, TNF-α, and TGF-β was higher in monocytes from individuals with moderate to severe fibrosis as compared to other groups. Although no differences were observed in receptors expression (IL-4R and IL-10R between groups of patients, the expression of IL-12 was lower in monocytes from individuals with moderate to severe fibrosis, suggesting a protective role of this cytokine in the development of fibrosis. Our data support the hypothesis that the three different monocyte populations participate in the immunopathogenesis of periportal fibrosis, since they express high levels of proinflammatory and profibrotic cytokines and low levels of regulatory markers.

  19. Mast cell subsets and neuropeptides in leprosy reactions

    Antunes Sérgio Luiz Gomes


    Full Text Available The immunohistochemical identification of neuropeptides (calcitonin gene-related peptide, vasoactive intestinal polypeptide, substance P, alpha-melanocyte stimulating hormone and gamma-melanocyte stimulating hormone quantification of mast cells and their subsets (tryptase/chymase-immunoreactive mast cells = TCMC and tryptase-immunoreactive mast cells = TMC were determined in biopsies of six patients with leprosy reactions (three patients with type I reaction and three with type II. Biopsies were compared with those taken from the same body site in the remission stage of the same patient. We found a relative increase of TMC in the inflammatory infiltrate of the reactional biopsies compared to the post-reactional biopsy. Also, the total number of mast cells and the TMC/TCMC ratio in the inflammatory infiltrate was significantly higher than in the intervening dermis of the biopsies of both periods. No significant difference was found regarding neuroptide expression in the reactional and post-reactional biopsies. The relative increase of TMC in the reactional infiltrates could implicate this mast cell subset in the reported increase of the immune response in leprosy reactions.

  20. Germination of embryos from stratified and non-stratified seeds and growth of apple seedlings (Malus domestica Borkh cv. "Antonówka"

    Jerzy Czerski


    Full Text Available The germination of whole seeds, the seeds without coat and isolated embryos of apple cv. "Antonówka Zwykła" after 90 days of cold-stratification was compared with the germination of embryos isolated from non-stratified seeds. They were germinated under 16hrs during a day at temperature 25°C and 20°C during the night. It has been found that after 2 weeks whole stratified seeds germinated in 5 per cent, seeds without coat in 25 per cent and isolated embryos in 98 per cent. Isolated embryos from nun-stratified seeds, after 2 weeks, germinated in the range from 75 to 88 per cent. The results indicate the similar germination ability of embryos isolated from nun-stratified seeds. The seedling populations obtained from embryo's stratified and non-stratified seeds were fully comparable and they evaluated: 1 a wide range of individual differences within population, 2 a similar number of seedlings in each class of shoot length, 3 a similar morphological habitus in each class of shoot length, 4 a similar fresh leaf weight and whole plant increment.

  1. Selecting Features of Single Lead ECG Signal for Automatic Sleep Stages Classification using Correlation-based Feature Subset Selection

    Ary Noviyanto


    Full Text Available Knowing about our sleep quality will help human life to maximize our life performance. ECG signal has potency to determine the sleep stages so that sleep quality can be measured. The data that used in this research is single lead ECG signal from the MIT-BIH Polysomnographic Database. The ECGs features can be derived from RR interval, EDR information and raw ECG signal. Correlation-based Feature Subset Selection (CFS is used to choose the features which are significant to determine the sleep stages. Those features will be evaluated using four different characteristic classifiers (Bayesian network, multilayer perceptron, IB1 and random forest. Performance evaluations by Bayesian network, IB1 and random forest show that CFS performs excellent. It can reduce the number of features significantly with small decreasing accuracy. The best classification result based on this research is a combination of the feature set derived from raw ECG signal and the random forest classifier.

  2. Pragmatic Implementation of a Stratified Primary Care Model for Low Back Pain Management in Outpatient Physical Therapy Settings: Two-Phase, Sequential Preliminary Study

    George, Steven Z.


    Background The effectiveness of risk stratification for low back pain (LBP) management has not been demonstrated in outpatient physical therapy settings. Objective The purposes of this study were: (1) to assess implementation of a stratified care approach for LBP management by evaluating short-term treatment effects and (2) to determine feasibility of conducting a larger-scale study. Design This was a 2-phase, preliminary study. Methods In phase 1, clinicians were randomly assigned to receive standard (n=6) or stratified care (n=6) training. Stratified care training included 8 hours of content focusing on psychologically informed practice. Changes in LBP attitudes and beliefs were assessed using the Pain Attitudes and Beliefs Scale for Physiotherapists (PABS-PT) and the Health Care Providers Pain and Impairment Relationship Scale (HC-PAIRS). In phase 2, clinicians receiving the stratified care training were instructed to incorporate those strategies in their practice and 4-week patient outcomes were collected using a numerical pain rating scale (NPRS), and the Oswestry Disability Index (ODI). Study feasibility was assessed to identify potential barriers for completion of a larger-scale study. Results In phase 1, minimal changes were observed for PABS-PT and HC-PAIRS scores for standard care clinicians (Cohen d=0.00–0.28). Decreased biomedical (−4.5±2.5 points, d=1.08) and increased biopsychosocial (+5.5±2.0 points, d=2.86) treatment orientations were observed for stratified care clinicians, with these changes sustained 6 months later on the PABS-PT. In phase 2, patients receiving stratified care (n=67) had greater between-group improvements in NPRS (0.8 points; 95% confidence interval=0.1, 1.5; d=0.40) and ODI (8.9% points; 95% confidence interval=4.1, 13.6; d=0.76) scores compared with patients receiving standard physical therapy care (n=33). Limitations In phase 2, treatment was not randomly assigned, and therapist adherence to treatment recommendations was

  3. Pragmatic Implementation of a Stratified Primary Care Model for Low Back Pain Management in Outpatient Physical Therapy Settings: Two-Phase, Sequential Preliminary Study.

    Beneciuk, Jason M; George, Steven Z


    The effectiveness of risk stratification for low back pain (LBP) management has not been demonstrated in outpatient physical therapy settings. The purposes of this study were: (1) to assess implementation of a stratified care approach for LBP management by evaluating short-term treatment effects and (2) to determine feasibility of conducting a larger-scale study. This was a 2-phase, preliminary study. In phase 1, clinicians were randomly assigned to receive standard (n=6) or stratified care (n=6) training. Stratified care training included 8 hours of content focusing on psychologically informed practice. Changes in LBP attitudes and beliefs were assessed using the Pain Attitudes and Beliefs Scale for Physiotherapists (PABS-PT) and the Health Care Providers Pain and Impairment Relationship Scale (HC-PAIRS). In phase 2, clinicians receiving the stratified care training were instructed to incorporate those strategies in their practice and 4-week patient outcomes were collected using a numerical pain rating scale (NPRS), and the Oswestry Disability Index (ODI). Study feasibility was assessed to identify potential barriers for completion of a larger-scale study. In phase 1, minimal changes were observed for PABS-PT and HC-PAIRS scores for standard care clinicians (Cohen d=0.00-0.28). Decreased biomedical (-4.5±2.5 points, d=1.08) and increased biopsychosocial (+5.5±2.0 points, d=2.86) treatment orientations were observed for stratified care clinicians, with these changes sustained 6 months later on the PABS-PT. In phase 2, patients receiving stratified care (n=67) had greater between-group improvements in NPRS (0.8 points; 95% confidence interval=0.1, 1.5; d=0.40) and ODI (8.9% points; 95% confidence interval=4.1, 13.6; d=0.76) scores compared with patients receiving standard physical therapy care (n=33). In phase 2, treatment was not randomly assigned, and therapist adherence to treatment recommendations was not monitored. This study was not adequately powered to

  4. Supernova feedback in a local vertically stratified medium: interstellar turbulence and galactic winds

    Martizzi, Davide; Faucher-Giguere, Claude-Andre; Quataert, Eliot


    We use local Cartesian simulations with a vertical gravitational potential to study how supernova (SN) feedback in stratified galactic discs drives turbulence and launches galactic winds. Our analysis includes three disc models with gas surface densities ranging from Milky Way-like galaxies to gas-rich ultra-luminous infrared galaxies (ULIRGs), and two different SN driving schemes (random and correlated with local gas density). In order to isolate the physics of SN feedback, we do not include additional feedback processes. We find that, in these local box calculations, SN feedback excites relatively low mass-weighted gas turbulent velocity dispersions ~3-7 km/s and low wind mass loading factors <1 in all the cases we study. The low turbulent velocities and wind mass loading factors predicted by our local box calculations are significantly below those suggested by observations of gas-rich and rapidly star-forming galaxies; they are also in tension with global simulations of disc galaxies regulated by stella...

  5. Swimming for survival: A role of phytoplankton motility in a stratified turbulent environment

    Ross, Oliver N.; Sharples, Jonathan

    We investigate a role for vertical migration in stratified coastal water, where the swimming speed is generally significantly less than the typical turbulent fluctuations in a tidally-mixed bottom layer. In our modelling approach we use a k- ɛ turbulence model to describe the physical forcing, a Lagrangian random walk model to describe the vertical displacement of individual cells in response to turbulence and due to cell motility, and a phytoplankton growth model to direct the swimming behaviour of the phytoplankton according to their light and nutrient requirements. The model results show how the cells form a stable subsurface chlorophyll maximum (SCM) at the base of the thermocline where episodic tidal turbulence causes erosion of part of the SCM biomass into the bottom mixed layer (BML). We then focus on the question of whether an ability to swim (weakly, compared to typical bottom layer turbulent intensities) provides any advantage by allowing return to the SCM. Our results show that tidal turbulence in the BML helps both motile and neutrally-buoyant cells by periodically pushing them into the base of the thermocline. Motile cells then have the advantage that they can swim further into the thermocline towards higher light which also reduces the likelihood of being re-mixed back into the BML.

  6. Acceptance of living liver donation among medical students: A multicenter stratified study from Spain

    Ríos, Antonio; López-Navas, Ana Isabel; López-López, Ana Isabel; Gómez, Francisco Javier; Iriarte, Jorge; Herruzo, Rafael; Blanco, Gerardo; Llorca, Francisco Javier; Asunsolo, Angel; Sánchez-Gallegos, Pilar; Gutiérrez, Pedro Ramón; Fernández, Ana; de Jesús, María Teresa; Martínez-Alarcón, Laura; Lana, Alberto; Fuentes, Lorena; Hernández, Juan Ramón; Virseda, Julio; Yelamos, José; Bondía, José Antonio; Hernández, Antonio Miguel; Ayala, Marco Antonio; Ramírez, Pablo; Parrilla, Pascual


    AIM: To analyze the attitude of Spanish medical students toward living liver donation (LLD) and to establish which factors have an influence on this attitude. METHODS: Study type: A sociological, interdisciplinary, multicenter and observational study. Study population: Medical students enrolled in Spain (n = 34000) in the university academic year 2010-2011. Sample size: A sample of 9598 students stratified by geographical area and academic year. Instrument used to measure attitude: A validated questionnaire (PCID-DVH RIOS) was self-administered and completed anonymously. Data collection procedure: Randomly selected medical schools. The questionnaire was applied to each academic year at compulsory sessions. Statistical analysis: Student´s t test, χ2 test and logistic regression analysis. RESULTS: The completion rate was 95.7% (n = 9275). 89% (n = 8258) were in favor of related LLD, and 32% (n = 2937) supported unrelated LLD. The following variables were associated with having a more favorable attitude: (1) age (P = 0.008); (2) sex (P mutilation of the body after donation (P < 0.001). CONCLUSION: Spanish medical students have a favorable attitude toward LLD. PMID:27433093

  7. Gambling problems in the family – A stratified probability sample study of prevalence and reported consequences

    Øren Anita


    Full Text Available Abstract Background Prior studies on the impact of problem gambling in the family mainly include help-seeking populations with small numbers of participants. The objective of the present stratified probability sample study was to explore the epidemiology of problem gambling in the family in the general population. Methods Men and women 16–74 years-old randomly selected from the Norwegian national population database received an invitation to participate in this postal questionnaire study. The response rate was 36.1% (3,483/9,638. Given the lack of validated criteria, two survey questions ("Have you ever noticed that a close relative spent more and more money on gambling?" and "Have you ever experienced that a close relative lied to you about how much he/she gambles?" were extrapolated from the Lie/Bet Screen for pathological gambling. Respondents answering "yes" to both questions were defined as Concerned Significant Others (CSOs. Results Overall, 2.0% of the study population was defined as CSOs. Young age, female gender, and divorced marital status were factors positively associated with being a CSO. CSOs often reported to have experienced conflicts in the family related to gambling, worsening of the family's financial situation, and impaired mental and physical health. Conclusion Problematic gambling behaviour not only affects the gambling individual but also has a strong impact on the quality of life of family members.


    Scott A. Socolofsky; Brian C. Crounse; E. Eric Adams


    Two-phase plumes play an important role in the more practical scenarios for ocean sequestration of CO{sub 2}--i.e. dispersing CO{sub 2} as a buoyant liquid from either a bottom-mounted or ship-towed pipeline. Despite much research on related applications, such as for reservoir destratification using bubble plumes, our understanding of these flows is incomplete, especially concerning the phenomenon of plume peeling in a stratified ambient. To address this deficiency, we have built a laboratory facility in which we can make fundamental measurements of plume behavior. Although we are using air, oil and sediments as our sources of buoyancy (rather than CO{sub 2}), by using models, our results can be directly applied to field scale CO{sub 2} releases to help us design better CO{sub 2} injection systems, as well as plan and interpret the results of our up-coming international field experiment. The experimental facility designed to study two-phase plume behavior similar to that of an ocean CO{sub 2} release includes the following components: 1.22 x 1.22 x 2.44 m tall glass walled tank; Tanks and piping for the two-tank stratification method for producing step- and linearly-stratified ambient conditions; Density profiling system using a conductivity and temperature probe mounted to an automated depth profiler; Lighting systems, including a virtual point source light for shadowgraphs and a 6 W argon-ion laser for laser induced fluorescence (LIF) imaging; Imaging system, including a digital, progressive scanning CCD camera, computerized framegrabber, and image acquisition and analysis software; Buoyancy source diffusers having four different air diffusers, two oil diffusers, and a planned sediment diffuser; Dye injection method using a Mariotte bottle and a collar diffuser; and Systems integration software using the Labview graphical programming language and Windows NT. In comparison with previously reported experiments, this system allows us to extend the parameter range of

  9. A model for evaluating the ballistic resistance of stratified packs

    Pirvu, C.; Georgescu, C.; Badea, S.; Deleanu, L.


    Models for evaluating the ballistic performance of stratified packs are useful in reducing the time for laboratory tests, understanding the failure process and identifying key factors to improve the architecture of the packs. The authors present the results of simulating the bullet impact on a packs made of 24 layers, taking into consideration the friction between layers (μ = 0.4) and the friction between bullet and layers (μ = 0.3). The aim of this study is to obtain a number of layers that allows for the bullet arrest in the packs and to have several layers undamaged in order to offer a high level of safety for this kind of packs that could be included in individual armors. The model takes into account the yield and fracture limits of the two materials the bullet is made of and those for one layer, here considered as an orthotropic material, having maximum equivalent plastic strain of 0.06. All materials are considered to have bilinear isotropic hardening behavior. After documentation, the model was designed as isothermal because thermal influence of the impact is considered low for these impact velocities. The model was developed with the help of Ansys 14.5. Each layer has 200 mm × 200 × 0.35 mm. The bullet velocity just before impact was 400 m/s, a velocity characterizing the average values obtained in close range with a ballistic barrel and the bullet model is following the shape and dimensions of the 9 mm FMJ (full metal jacket). The model and the results concerning the number of broken layers were validated by experiments, as the number of broken layers for the actual pack (made of 24 layers of LFT SB1) were also seven...eight. The models for ballistic impact are useful when they are particularly formulated for resembling to the actual system projectile - target.

  10. Internal and vorticity waves in decaying stratified flows

    Matulka, A.; Cano, D.


    Most predictive models fail when forcing at the Rossby deformation Radius is important and a large range of scales have to be taken into account. When mixing of reactants or pollutants has to be accounted, the range of scales spans from hundreds of Kilometers to the Bachelor or Kolmogorov sub milimiter scales. We present some theoretical arguments to describe the flow in terms of the three dimensional vorticity equations, using a lengthscale related to the vorticity (or enstrophy ) transport. Effect of intermittent eddies and non-homogeneity of diffusion are also key issues in the environment because both stratification and rotation body forces are important and cause anisotropy/non-homogeneity. These problems need further theoretical, numerical and observational work and one approach is to try to maximize the relevant geometrical information in order to understand and therefore predict these complex environmental dispersive flows. The importance of the study of turbulence structure and its relevance in diffusion of contaminants in environmental flows is clear when we see the effect of environmental disasters such as the Prestige oil spill or the Chernobil radioactive cloud spread in the atmosphere. A series of Experiments have been performed on a strongly stratified two layer fluid consisting of Brine in the bottom and freshwater above in a 1 square meter tank. The evolution of the vortices after the passage of a grid is video recorded and Particle tracking is applied on small pliolite particles floating at the interface. The combination of internal waves and vertical vorticity produces two separate time scales that may produce resonances. The vorticity is seen to oscilate in a complex way, where the frecuency decreases with time.

  11. The nonlinear evolution of modes on unstable stratified shear layers

    Blackaby, Nicholas; Dando, Andrew; Hall, Philip


    The nonlinear development of disturbances in stratified shear flows (having a local Richardson number of value less than one quarter) is considered. Such modes are initially fast growing but, like related studies, we assume that the viscous, non-parallel spreading of the shear layer results in them evolving in a linear fashion until they reach a position where their amplitudes are large enough and their growth rates have diminished sufficiently so that amplitude equations can be derived using weakly nonlinear and non-equilibrium critical-layer theories. Four different basic integro-differential amplitude equations are possible, including one due to a novel mechanism; the relevant choice of amplitude equation, at a particular instance, being dependent on the relative sizes of the disturbance amplitude, the growth rate of the disturbance, its wavenumber, and the viscosity of the fluid. This richness of choice of possible nonlinearities arises mathematically from the indicial Frobenius roots of the governing linear inviscid equation (the Taylor-Goldstein equation) not, in general, differing by an integer. The initial nonlinear evolution of a mode will be governed by an integro-differential amplitude equations with a cubic nonlinearity but the resulting significant increase in the size of the disturbance's amplitude leads on to the next stage of the evolution process where the evolution of the mode is governed by an integro-differential amplitude equations with a quintic nonlinearity. Continued growth of the disturbance amplitude is expected during this stage, resulting in the effects of nonlinearity spreading to outside the critical level, by which time the flow has become fully nonlinear.

  12. A new scoring system to stratify risk in unstable angina

    Salzberg Simón


    Full Text Available Abstract Background We performed this study to develop a new scoring system to stratify different levels of risk in patients admitted to hospital with a diagnosis of unstable angina (UA, which is a complex syndrome that encompasses different outcomes. Many prognostic variables have been described but few efforts have been made to group them in order to enhance their individual predictive power. Methods In a first phase, 473 patients were prospectively analyzed to determine which factors were significantly associated with the in-hospital occurrence of refractory ischemia, acute myocardial infarction (AMI or death. A risk score ranging from 0 to 10 points was developed using a multivariate analysis. In a second phase, such score was validated in a new sample of 242 patients and it was finally applied to the entire population (n = 715. Results ST-segment deviation on the electrocardiogram, age ≥ 70 years, previous bypass surgery and troponin T ≥ 0.1 ng/mL were found as independent prognostic variables. A clear distinction was shown among categories of low, intermediate and high risk, defined according to the risk score. The incidence of the triple end-point was 6 %, 19.2 % and 44.7 % respectively, and the figures for AMI or death were 2 %, 11.4 % and 27.6 % respectively (p Conclusions This new scoring system is simple and easy to achieve. It allows a very good stratification of risk in patients having a clinical diagnosis of UA. They may be divided in three categories, which could be of help in the decision-making process.

  13. Assessing iron dynamics in the release from a stratified reservoir

    Ashby, S.L.; Faulkner, S.P.; Gambrell, R.P.; Smith, B.A.


    Field and laboratory studies were conducted to describe the fate of total, dissolved, and ferrous (Fe2.) iron in the release from a stratified reservoir with an anoxic hypolimnion. Concentrations of total iron in the tail water indicated a first order removal process during a low flow release (0.6 m3sec1), yet negligible loss was observed during a period of increased discharge (2.8 m 3 sec-1). Dissolved and ferrous iron concentrations in the tailwater were highly variable during both release regimes and did not follow responses based on theoretical predictions. Ferrous iron concentrations in unfiltered samples were consistently greater than concentrations observed in samples filtered separately through 0.4, 0.2, and 0.1 ??m filters. Total iron removal in laboratory studies followed first order kinetics, but was twice that rate (0.077 mg.L-1 .hr 1) observed during low flow discharge in the tailwater (0.036 mg. L1 .hr1). Dissolved and ferrous iron losses in laboratory studies were rapid (???75% in the first 15 minutes and 95% within 1 hour), followed theoretical predictions, and were much faster than observations in the tailwater (???30% within the first hour). The presence of particulate forms of ferrous iron in the field and differences in removal rates observed in field and laboratory studies indicate a need for improved field assessment techniques and consideration of complexation reactions when assessing the dynamics of iron in reservoir releases and downstream impacts as a result of operation regimes. ?? Copyright by the North American Lake Management Society 2004.

  14. Interfacial instabilities in a stratified flow of two superposed fluids

    Schaflinger, Uwe


    Here we shall present a linear stability analysis of a laminar, stratified flow of two superposed fluids which are a clear liquid and a suspension of solid particles. The investigation is based upon the assumption that the concentration remains constant within the suspension layer. Even for moderate flow-rates the base-state results for a shear induced resuspension flow justify the latter assumption. The numerical solutions display the existence of two different branches that contribute to convective instability: long and short waves which coexist in a certain range of parameters. Also, a range exists where the flow is absolutely unstable. That means a convectively unstable resuspension flow can be only observed for Reynolds numbers larger than a lower, critical Reynolds number but still smaller than a second critical Reynolds number. For flow rates which give rise to a Reynolds number larger than the second critical Reynolds number, the flow is absolutely unstable. In some cases, however, there exists a third bound beyond that the flow is convectively unstable again. Experiments show the same phenomena: for small flow-rates short waves were usually observed but occasionally also the coexistence of short and long waves. These findings are qualitatively in good agreement with the linear stability analysis. Larger flow-rates in the range of the second critical Reynolds number yield strong interfacial waves with wave breaking and detached particles. In this range, the measured flow-parameters, like the resuspension height and the pressure drop are far beyond the theoretical results. Evidently, a further increase of the Reynolds number indicates the transition to a less wavy interface. Finally, the linear stability analysis also predicts interfacial waves in the case of relatively small suspension heights. These results are in accordance with measurements for ripple-type instabilities as they occur under laminar and viscous conditions for a mono-layer of particles.

  15. Magnetoacoustic Waves in Stratified Atmospheres with a Magnetic Null Point

    Tarr, Lucas A.; Linton, Mark; Leake, James E.


    Magnetic fields strongly modify the propagation of MHD waves from the photosphere to the low corona, as can be shown exactly for the most simple case of a uniform magnetic field and isothermally stratrified atmosphere. For slightly more realistic scenarios, where both the atmospheric parameters and the magnetic field vary spatially, the linear MHD equations typically cannot be solved analytically. We use the Lagrangian Remap code--a nonlinear, shock-capturing MHD code--to study the propagation of initially acoustic wavepackets through a model 2D atmosphere that includes a gravitationally stratified chromosphere, transition region, and low corona. The magnetic field is formed by three photospheric concentrations and includes a single magnetic null point, resulting in an inhomogeneous system with a magnetic dome topology. A portion of an introduced wavepacket will refract toward the null due to the varying Alfven speed. Waves incident on the equipartition contour surrounding the null, where the sound and Alfven speeds coincide, partially transmit, reflect, and mode convert between branches of the local dispersion relation. Outward propagating slow modes generated during conversion become strongly concentrated along the set of field lines passing near the null. Acoustic energy is beamed back downwards towards each photospheric foot point, and upwards along one separatrix that exits the top of the numerical domain. Changes in the dominant restoring force for the wavepacket, between the Lorentz and pressure gradient forces, lead to a buildup of current density along topologically important features of the system (the null point and its four separatrices) and can drive reconnection at the null point itself. Ohmic dissipation of the currents locally heats the plasma. We find that the amount of current accumulation depends on where the centroid of a wavepacket initial crosses the photosphere, but does not simply coincide with regions of open versus closed magnetic field or

  16. Stability characteristics of jets in linearly-stratified, rotating fluids

    Chen, Rui-Rong; Boyer, Don L.; Tao, Lijun

    A series of laboratory experiments are conducted concerning an azimuthal jet of a linearly stratified rotating fluid in a cylindrical geometry. The jet is characterized by vertical and horizontal shear and the question of the stability of the flow is considered experimentally. The jet is driven by a source-sink method characterized by a volume flow rate of strength Q. BecauseQ has no direct geophysical significance a combined external set of dimensionless parameters is introduced. These include the Rossby, Richardson and Ekman numbers, the jet aspect ratio and two geometrical parameters. A RossbyRo against RichardsonRi number flow regime diagram is presented which shows that the wave mode of the instability generally decreases with increasingRo andRi, for fixedRi andRo, respectively. In accordance with Killworth's (1980) linear stability analysis, the wave mode for smallRi (Ri ⪉ 15) depends principally onRi with the instability being largely a baroclinic one. For largerRi(Ri ⪉ 100), again as predicted by Killworth's theory, the wave mode depends primarily onRo, the instability being a barotropic one. The regime diagram can be used to estimate the wave-length of jet instabilities in the atmosphere and oceans. These estimates suggest that the wave-lengths decrease with increasing jet velocity, decreasing jet width (equivalent to increasing horizontal shear) and increasing vertical shear, other parameters being fixed. An azimuthal topography aligned along the jet has the tendency to stabilize the jet in the sense that the amplitude of the instability is shown to be dramatically smaller in the presence of the topography, other parameters being fixed. The topography also tends to increase the wave-length of the instability. A scaling analysis is advanced, and supporting experimental data presented, relating the external and internal parameters utilized.

  17. Changes in T lymphocyte subsets after severe traumatic brain injury

    Yulu Miao; Mingxia Zhang; Yulin Nie; Wan Zhao; Bin Huang; Zhengming Jiang; Shaoxiong Yu; Zhibin Huang; Hongjin Fu


    BACKGROUND: Besides local changes of cranial parenchymal cells, hemorrhage, etc., severe traumatic brain injuries also cause the changes of total body fluid and various functions, and the changes of lymphocytes and T lymphocyte subsets should be paid more attention to.OBJECTIVE: To reveal the changing laws of T lymphocyte subsets after severe traumatic brain injury, and compare with mild to moderate brain injury.DESIGN: A comparative observation.SETTINGS: Department of Neurosurgery, Longgang District Buji People's Hospital of Shenzhen City;Central Laboratory of Shenzhen Hospital of Prevention and Cure for Chronic Disease.PARTICIPANTS: All the subjects were selected from the Department of Neurosurgery, Longgang District Buji People's Hospital of Shenzhen City from August 2002 to August 2005. Thirty patients with severe brain injury, whose Glasgow coma score (GCS) was ≤ 8 points, were taken as the experimental group, including 21 males and 9 females, aging 16 - 62 years. Meanwhile, 30 patients with mild traumatic brain injury were taken as the control group (GCS ranged 14 - 15 points), including 18 males and 12 females, aging 15 - 58 years. All the subjects were in admission at 6 hours after injury, without disease of major organs before injury.Informed consents were obtained from all the patients or their relatives.conditions of pulmonaryinfections were observed at 4 days after injury. The differences of measurement data were compared with the t test.MAIN OUTCOME MEASURES: Changes of T lymphocytes subsets at 1 - 14 days after severe and mild or moderate traumatic injury.RESULTS: Finally, 28 and 25 patients with mild to moderate traumatic brain injury, whereas 25 and 21 patients with severe traumatic brain injury were analyzed at 7 and 14 days respectively, and the missed ones CD3, CD4, CD8, CD4/CD8 began to decrease, whereas CD8 increased in the experimental group, which were very significantly different from those in the control group (t =2.77 - 3.26, P < 0

  18. mIL-2R, T cell subsets & hepatitis C

    Chao-Pin Li; Ke-Xia Wang; Jian Wang; Bo-Rong Pan


    AIM: To study the levels of membrane interleukin-2 receptor(mIL-2R ) and T cell subsets in peripheral bloodmononuclear cells (PBMC) from patients with hepatitis Cand their role in the pathogenesis of hepatitis C.METHODS: The levels of mlL-2R and T cells subsets in PBMCWere detected by biotin- streptstividin (BSA) technique beforeand after stimulation with PHA in 203 patients with hepatitis Cwith HCV-RNA( + ), anti-HCV( + ), anti-HCV(-).RESULTS: The total expressive levels of mlL-2R before andafter stimulation with PHA(0.03 ± 0.01, 0.03 ± 0.02, 0.04 ± 0.02, 0.36±0.03), and Tcell subsets in PBMC (0.62±0.06,0.37 ± 0.05, 0.35 ± 0.07) were all lower in patients withhepatitis C than those in normal controls (0.66 ± 0.07, 0.41± 0.06, 0.31 ± 0.05, P < 0.01 ). Among the patients, thelevels of mlL-2R were lower in silence than those in situationof PHA inducting (P< 0.01). However, the levels of mlL-2Rwere similar in acute hepatitis C to that in chronic hepatitis C(P>0.05). The levels of CD3+, CD4+, CD4 +/CD8+ Were lov erand CD8 + was higher in patients with acute and chronichepatitis C with anti-HCV( + ) than those in normal controls (0.62±0.06, 0.37±0.05, 0.35±0.07, 1.18±0.30, 0.61±0.07, 0.37±0.05, 1.39±0.33, 0.31±0.05, P<0.05-P<0.01).CONCLUSION: The cellular immunity is obviously changed inpatients with hepatitis C. The levels of mlL-2R end activationof T cells am closely associated with chronicity of hepatitis C.

  19. Distribution of vaccine/antivirals and the 'least spread line' in a stratified population

    Goldstein, E.; Apolloni, A.; Lewis, B.; Miller, J. C.; Macauley, M.; Eubank, S.; Lipsitch, M.; Wallinga, J.


    We describe a prioritization scheme for an allocation of a sizeable quantity of vaccine or antivirals in a stratified population. The scheme builds on an optimal strategy for reducing the epidemic's initial growth rate in a stratified mass-action model. The strategy is tested on the EpiSims network

  20. Implementing content constraints in alpha-stratified adaptive using a shadow test approach

    Linden, van der Wim J.; Chang, Hua-Hua


    The methods of alpha-stratified adaptive testing and constrained adaptive testing with shadow tests are combined. The advantages are twofold: First, application of the shadow test approach allows the implementation of any type of constraint on item selection in alpha-stratified adaptive testing. Sec

  1. Implementing Content Constraints in Alpha-Stratified Adaptive Testing Using a Shadow Test Approach. Research Report.

    van der Linden, Wim J.; Chang, Hua-Hua

    The methods of alpha-stratified adaptive testing and constrained adaptive testing with shadow tests are combined in this study. The advantages are twofold. First, application of the shadow test allows the researcher to implement any type of constraint on item selection in alpha-stratified adaptive testing. Second, the result yields a simple set of…

  2. Experimental Validation of a Domestic Stratified Hot Water Tank Model in Modelica for Annual Performance Assessment

    Carmo, Carolina; Dumont, Olivier; Nielsen, Mads Pagh


    The use of stratified hot water tanks in solar energy systems - including ORC systems - as well as heat pump systems is paramount for a better performance of these systems. However, the availability of effective and reliable models to predict the annual performance of stratified hot water tanks c...

  3. Lessons for molecular diagnostics in oncology from the Cancer Research UK Stratified Medicine Programme.

    Lindsay, Colin R; Shaw, Emily; Walker, Ian; Johnson, Peter W M


    The implementation of stratified medicine in modern cancer care presents substantial opportunity to refine diagnosis and treatment but also numerous challenges. Through experience in a UK tumor profiling initiative, we have gained valuable insights into the complexities and possible solutions for routine delivery of stratified cancer medicine.

  4. Optimal stratification of item pools in α-stratified computerized adaptive testing

    Chang, Hua-Hua; Linden, van der Wim J.


    A method based on 0-1 linear programming (LP) is presented to stratify an item pool optimally for use in α-stratified adaptive testing. Because the 0-1 LP model belongs to the subclass of models with a network flow structure, efficient solutions are possible. The method is applied to a previous item

  5. Urofacial syndrome: A subset of neurogenic bladder dysfunction syndromes?

    Stamatiou, K. N.; Karakos, C. D.


    The urofacial syndrome is probably a subset of neurogenic bladder dysfunction syndromes characterized by detrusor-sphincter discoordination along with a characteristic inversion of facial expression with laughing. This characteristic facial expression can facilitate early detection of this disorder, which leads to poor bladder emptying with high residual urine, hydro-nephrosis with vesico-ureteral reflux and potentially renal failure if left untreated. The etiology of the urofacial syndrome is unknown. In our case, a 12-year-old boy of Middle-Eastern origin presented to the Outpatient Department of our hospital with left pyelonephritis, hydronephrosis and bladder dilatation. Voiding cystourethrography performed 15 days later revealed left vesicoureteral reflux. Cystoscopy revealed bladder trabeculation however an anatomic urethral obstruction was not noticed. Both, neurological examination and radiography of the lumbosacral spine were normal. Urodynamic evaluation revealed the typical findings of detrusor-sphincter discoordination. PMID:21369396

  6. REST represses a subset of the pancreatic endocrine differentiation program

    Martin, David; Kim, Yung-Hae; Sever, Dror


    To contribute to devise successful beta-cell differentiation strategies for the cure of Type 1 diabetes we sought to uncover barriers that restrict endocrine fate acquisition by studying the role of the transcriptional repressor REST in the developing pancreas. Rest expression is prevented...... in neurons and in endocrine cells, which is necessary for their normal function. During development, REST represses a subset of genes in the neuronal differentiation program and Rest is down-regulated as neurons differentiate. Here, we investigate the role of REST in the differentiation of pancreatic...... endocrine cells, which are molecularly close to neurons. We show that Rest is widely expressed in pancreas progenitors and that it is down-regulated in differentiated endocrine cells. Sustained expression of REST in Pdx1(+) progenitors impairs the differentiation of endocrine-committed Neurog3...

  7. Plasticity of T helper cell subsets: Implications in periodontal disease

    Avaneendra Talwar


    Full Text Available T helper (Th cells have an important role in host defence as well in the pathogenesis of periodontal disease. Th cells differentiate from naive cells into various subsets, each of which is associated with a set of inducing and effector cytokines. Previously, it was thought that this differentiation was an irreversible event. Recent evidence suggest that even differentiated Th cells, retain the flexibility to transform from one lineage to another, a phenomenon referred to as plasticity. This plasticity is thought to be brought about by epigenetic modifications that are regulated by external and internal signals in the micro-environment of these cells. The factors and mechanisms which affect the plasticity of these cells and their potential role in the etio-pathogenesis of periodontal disease has been described in this article.

  8. On curves contained in convex subsets of the plane

    Coppersmith, Don; Ravsky, Alex


    If K' and K are convex bodies of the plane such that K' is a subset of K then the perimeter of K' is not greater than the perimeter of K. We obtain the following generalization of this fact. Let K be a convex compact body of the plane with the perimeter p and the diameter d and r>1 be an integer. Let s be the smallest number such that for any curve of length greater than s contained in K there is a straight line intersecting the curve at least in r+1 different points. Then s=rp/2 if r is even and s=(r-1)p/2+d if r is odd.

  9. Seronegative necrolytic acral erythema: A distinct clinical subset?

    Panda S


    Full Text Available A patient was referred to us with asymptomatic, erythematous, nonitchy, scaly lesions present bilaterally on the dorsa of his feet and toes since the last 2 months. Both the legs had pitting edema as well. There were hyperkeratosis, focal parakeratosis, acanthosis and scattered spongiosis in the epidermis, and proliferation of capillaries with perivascular infiltration of lymphomononuclear cells in the dermis. There was no serological evidence of hepatitis C virus. Laboratory investigations revealed hypoalbuminemia and low-normal serum zinc. On clinicopathological correlation, we made a diagnosis of necrolytic acral erythema (NAE. The lesions responded dramatically to oral zinc sulfate and topical clobetasol propionate within 3 weeks with disappearance of edema and scaling and only a minimal residual erythema. This is the first reported case of NAE from Eastern India. NAE with negative serology for hepatitis C may be viewed as a distinct subset of the condition that had been originally described.

  10. The semigroup of nonempty finite subsets of rationals

    Reuben Spake


    Full Text Available Let Q be the additive group of rational numbers and let ℛ be the additive semigroup of all nonempty finite subsets of Q. For X∈ℛ, define AX to be the basis of 〈X−min(X〉 and BX the basis of 〈max(X−X〉. In the greatest semilattice decomposition of ℛ, let (X denote the archimedean component containing X. In this paper we examine the structure of ℛ and determine its greatest semilattice decomposition. In particular, we show that for X,Y∈ℛ, (X=(Y if and only if AX=AY and BX=BY. Furthermore, if X is a non-singleton, then the idempotent-free (X is isomorphic to the direct product of a power joined subsemigroup and the group Q.

  11. T-lymphocyte subsets, thymic size and breastfeeding in infancy

    Jeppesen, Dorthe Lisbeth; Hasselbalch, Helle; Lisse, Ida M


    We followed the changes in concentration of T-lymphocyte subsets (CD4+ and CD8+ cells) in peripheral blood and thymus size during infancy. Previous studies have found increased thymus size in breastfed infants. The present study analyzed the association between breastfeeding and the number of CD4......+ and CD8+ cells. Two different populations of infants between birth and 1 year of age were examined. Study Group I: infants with a variable duration of breastfeeding. Study Group II: long-term breastfed infants. In both groups a correlation was found between CD8+ cells and the thymic index at 10 months...... to 10 months of age; and a positive correlation between the number of breastfeedings per day at 8 months of age, and an increase in CD4+ cells from 8 to 10 months of age (p Breastfeeding might have both a current and long...

  12. Urofacial syndrome: A subset of neurogenic bladder dysfunction syndromes?

    K N Stamatiou


    Full Text Available The urofacial syndrome is probably a subset of neurogenic bladder dysfunction syndromes characterized by detrusor-sphincter discoordination along with a characteristic inversion of facial expression with laughing. This characteristic facial expression can facilitate early detection of this disorder, which leads to poor bladder emptying with high residual urine, hydro-nephrosis with vesico-ureteral reflux and potentially renal failure if left untreated. The etiology of the urofacial syndrome is unknown. In our case, a 12-year-old boy of Middle-Eastern origin presented to the Outpatient Department of our hospital with left pyelonephritis, hydronephrosis and bladder dilatation. Voiding cystourethrography performed 15 days later revealed left vesicoureteral reflux. Cystoscopy revealed bladder trabeculation however an anatomic urethral obstruction was not noticed. Both, neurological examination and radiography of the lumbosacral spine were normal. Urodynamic evaluation revealed the typical findings of detrusor-sphincter discoordination.

  13. Bifidobacterium infantis attenuates colitis by regulating T cell subset responses

    Zuo, Li; Yuan, Kai-Tao; Yu, Li; Meng, Qing-Hong; Chung, Peter Chee-Keung; Yang, Ding-Hua


    AIM: to investigate the effect of Bifidobacterium infantis (B. infantis) on the T cell subsets and in attenuating the severity of experimental colitis in mice. METHODS: Normal BALB/c mice were fed different doses of B. infantis for 3 wk, and T cell subsets and related cytokine profiles in mesenteric lymph nodes (MLNs) were detected by flow cytometry and real-time RT-PCR. Colitis was induced by administration of trinitrobenzene sulfonic acid (TNBS) in mice. Before colitis induction, mice were fed high dose B. infantis for 3 wk. Cytokine profiles in MLNs and histological changes of colonic tissue were examined 6 d after colitis induction. RESULTS: No significant change in cytokine profiles was observed in normal mice fed low dose B. infantis. However, Th1-related cytokines (IL-2, IFN-γ, IL-12p40), Th17-related transcription factor and cytokines (RORγt, IL-21, IL-23), regulatory T cell (Treg)-related transcription factor and cytokines (Foxp3, IL-10) were increased in normal mice fed high dose B. infantis. Furthermore, flow cytometry assay showed B. infantis increased the numbers of CD4+Foxp3+ Tregs and Th17 cells in MLNs. Colitis was successfully induced by TNBS in mice, characterized by colonic inflammation and aberrant Th1 and Th17 responses. Feeding high dose B. infantis for 3 wk before colitis induction decreased the inflammatory cell infiltration and goblet cell depletion and restored the intestinal epithelium. In addition, B. infantis feeding reduced Th1-related cytokines (T-bet, IL-2 and IFN-γ) and Th17-related cytokines (IL-12p40, RORγt, IL-17A, IL-21 and IL-23), and increased Treg-related molecules (Foxp3, IL-10 and TGF-β) in colitis mice. CONCLUSION: B. infantis effectively attenuates TNBS-induced colitis by decreasing Th1 and Th17 responses and increasing Foxp3+ Treg response in the colonic mucosa. PMID:25561798

  14. Thermal hypersensitivity in a subset of irritable bowel syndrome patients

    QiQi Zhou; Roger B Fillingim; Joseph L Riley III; G Nicholas Verne


    AIM: To characterize thermal hypersensitivity in patients with constipation- and diarrhea-predominant irritable bowel syndrome (IBS). METHODS: Thermal pain sensitivity was tested among patients with diarrhea-predominant IBS (D-IBS) and constipation-predominant IBS (C-IBS) compared to healthy subjects. A total of 42 patients (29 female and patients (16 female and eight male; mean age 32.5participated in the study. Thermal stimuli were delivered using a Medoc Thermal Sensory Analyzer with a 3 cm heat pain tolerance (HPTo) were assessed on the left ventral forearm and left calf using an ascending method of limits. The Functional Bowel Disease Severity Index (FBDSI) was also obtained for all subjects. RESULTS: Controls were less sensitive than C-IBS and D-IBS (both at P < 0.001) with no differences between C-IBS and D-IBS for HPTh and HPTo. Thermal hyperalgesia was present in both groups of IBS patients relative to controls, with IBS patients reporting significantly lower pain threshold and pain tolerance at both test sites. Cluster analysis revealed the presence of subgroups of IBS patients based on thermal hyperalgesia. One cluster (17% of the sample) showed a profile of heat pain sensitivity very similar to that of healthy controls; a second cluster (47% of the sample) showed moderate heat pain sensitivity; and a third cluster (36% of the sample) showed a very high degree of thermal hyperalgesia.CONCLUSION: A subset of IBS patients had thermal hypersensitivity compared to controls, who reported significantly lower HPTh and HPTo. All IBS patients had a higher score on the FBDSI than controls. Interestingly, the subset of IBS patients with high thermal sensitivity (36%) had the highest FBDSI score compared to the other two groups of IBS patients.

  15. Dysregulation of CD4(+) T Cell Subsets in Intracranial Aneurysm.

    Zhang, Hai-Feng; Zhao, Ming-Guang; Liang, Guo-Biao; Yu, Chun-Yong; He, Wenxiu; Li, Zhi-Qing; Gao, Xu


    Intracranial aneurysms (IAs) and potential IA rupture are one of the direct causes of permanent brain damage and mortality. Interestingly, the major risk factors of IA development, including hemodynamic stress, hypertension, smoking, and genetic predispositions, are closely associated with a proinflammatory immune status. Therefore, we examined the roles of CD4(+) T cells in IA pathogenesis. IA patients exhibited peripheral CD4(+) T-cell imbalance, with overrepresented T helper 1 (Th1) and Th17 activities and underrepresented Th2 and regulatory T (Treg) activities, including increased IFN-γ, TNF-α, and IL-17 production and decreased IL-10 production from total CD4(+) T cells. Chemokine receptors CXCR3 and CCR6 were used to identify Th1, Th2, and Th17 cell subsets, and CD4(+)CD25(hi) was used to identify Treg cells. Based on these markers, the data then showed altered cytokine production by each cell type and shifted subpopulation frequency. Moreover, this shift in frequency was directly correlated with IA severity. To examine the underlying mechanism of CD4(+) T cell skewing, we cocultured CD4(+) T cells with autologous monocytes and found that coculture with monocytes could significantly increase IFN-γ and IL-17 production through contact-independent mechanisms, demonstrating that monocytes could potentially contribute to the altered CD4(+) T cell composition in IA. Analyzing mRNA transcripts revealed significantly upregulated IL-1β and TNF-α expression by monocytes from IA patients. We found a loss of CD4(+) T cell subset balance that was likely to promote a higher state of inflammation in IA, which may exacerbate the disease through a positive feedback loop.

  16. Tachykinins stimulate a subset of mouse taste cells.

    Jeff Grant

    Full Text Available The tachykinins substance P (SP and neurokinin A (NKA are present in nociceptive sensory fibers expressing transient receptor potential cation channel, subfamily V, member 1 (TRPV1. These fibers are found extensively in and around the taste buds of several species. Tachykinins are released from nociceptive fibers by irritants such as capsaicin, the active compound found in chili peppers commonly associated with the sensation of spiciness. Using real-time Ca(2+-imaging on isolated taste cells, it was observed that SP induces Ca(2+ -responses in a subset of taste cells at concentrations in the low nanomolar range. These responses were reversibly inhibited by blocking the SP receptor NK-1R. NKA also induced Ca(2+-responses in a subset of taste cells, but only at concentrations in the high nanomolar range. These responses were only partially inhibited by blocking the NKA receptor NK-2R, and were also inhibited by blocking NK-1R indicating that NKA is only active in taste cells at concentrations that activate both receptors. In addition, it was determined that tachykinin signaling in taste cells requires Ca(2+-release from endoplasmic reticulum stores. RT-PCR analysis further confirmed that mouse taste buds express NK-1R and NK-2R. Using Ca(2+-imaging and single cell RT-PCR, it was determined that the majority of tachykinin-responsive taste cells were Type I (Glial-like and umami-responsive Type II (Receptor cells. Importantly, stimulating NK-1R had an additive effect on Ca(2+ responses evoked by umami stimuli in Type II (Receptor cells. This data indicates that tachykinin release from nociceptive sensory fibers in and around taste buds may enhance umami and other taste modalities, providing a possible mechanism for the increased palatability of spicy foods.

  17. Helicity, geostrophic balance and mixing in rotating stratified turbulence: a multi-scale problem

    Pouquet, A.; Marino, R.; Mininni, P.; Rorai, C.; Rosenberg, D. L.


    Helicity, geostrophic balance and mixing in rotating stratified turbulence: a multi-scale problem A. Pouquet, R. Marino, P. D. Mininni, C. Rorai & D. Rosenberg, NCAR Interactions between winds and waves have important roles in planetary and oceanic boundary layers, affecting momentum, heat and CO2 transport. Within the Abyssal Southern Ocean at Mid latitude, this may result in a mixed layer which is too shallow in climate models thereby affecting the overall evolution because of poor handling of wave breaking as in Kelvin-Helmoltz instabilities: gravity waves couple nonlinearly on slow time scales and undergo steepening through resonant interactions, or due to the presence of shear. In the oceans, sub-mesoscale frontogenesis and significant departure from quasi-geostrophy can be seen as turbulence intensifies. The ensuing anomalous vertical dispersion may not be simply modeled by a random walk, due to intermittent structures, wave propagation and to their interactions. Conversely, the energy and seeds required for such intermittent events to occur, say in the stable planetary boundary layer, may come from the wave field that is perturbed, or from winds and the effect of topography. Under the assumption of stationarity, weak nonlinearities, dissipation and forcing, one obtains large-scale geostrophic balance linking pressure gradient, gravity and Coriolis force. The role of helicity (velocity-vorticity correlations) has not received as much attention, outside the realm of astrophysics when considering the growth of large-scale magnetic fields. However, it is measured routinely in the atmosphere in order to gauge the likelihood of supercell convective storms to strengthen, and it may be a factor to consider in the formation of hurricanes. In this context, we examine the transition from a wave-dominated regime to an isotropic small-scale turbulent one in rotating flows with helical forcing. Using a direct numerical simulation (DNS) on a 3072^3 grid with Rossby and

  18. Feature Subset Selection for Hot Method Prediction using Genetic Algorithm wrapped with Support Vector Machines

    S. Johnson


    Full Text Available Problem statement: All compilers have simple profiling-based heuristics to identify and predict program hot methods and also to make optimization decisions. The major challenge in the profile-based optimization is addressing the problem of overhead. The aim of this work is to perform feature subset selection using Genetic Algorithms (GA to improve and refine the machine learnt static hot method predictive technique and to compare the performance of the new models against the simple heuristics. Approach: The relevant features for training the predictive models are extracted from an initial set of randomly selected ninety static program features, with the help of the GA wrapped with the predictive model using the Support Vector Machine (SVM, a Machine Learning (ML algorithm. Results: The GA-generated feature subsets containing thirty and twenty nine features respectively for the two predictive models when tested on MiBench predict Long Running Hot Methods (LRHM and frequently called hot methods (FCHM with the respective accuracies of 71% and 80% achieving an increase of 19% and 22%. Further, inlining of the predicted LRHM and FCHM improve the program performance by 3% and 5% as against 4% and 6% with Low Level Virtual Machines (LLVM default heuristics. When intra-procedural optimizations (IPO are performed on the predicted hot methods, this system offers a performance improvement of 5% and 4% as against 0% and 3% by LLVM default heuristics on LRHM and FCHM respectively. However, we observe an improvement of 36% in certain individual programs. Conclusion: Overall, the results indicate that the GA wrapped with SVM derived feature reduction improves the hot method prediction accuracy and that the technique of hot method prediction based optimization is potentially useful in selective optimization.

  19. Value of the peripheral blood B-cells subsets in patients with ankylosing spondylitis

    LIN Qu; GU Jie-ruo; LI Tian-wang; ZHANG Fu-cheng; LIN Zhi-ming; LIAO Ze-tao; WEI Qiu-jing; CAO Shuang-yan; LI Li


    Background The role of B-cell remains an enigma in the pathogenesis of ankylosing spondylitis(AS).This study aimed to investigate the distributions of B-cells and subsets in peripheral blood of AS patients and observe their changes in etanercept-treated AS patents.Methods We detected the proportions of CD19+ B-cell,naive B-cell(CD19+CD27),memory B-cell(CD19+CD27dim)and plasmablast(CD19+CD27high)in peripheral blood of 66 patients with AS(39 at active stage,27 at stable stage;35patients with peripheral joint involvement,31 patients with axial involvement alone),30 patients with rheumatoid arthritis (RA)and 30 healthy volunteers using flow cytometry.And then we observedthe changes of the above indexes of 39 active AS patients treated with etanercept in a randomized,double-blind,placebo-controlled trial.Results (1)Percentages of CD19+ B-cells in active or peripheral joint involvement AS patients increased more obviously than those in stable or axial involvement alone AS patients(both P=0.001),and percentage of CD19+CD27high B-cells in AS patients with peripheral joint involvement was significantly higher than that in cases with axial involvement alone or healthy volunteers(P=0.005 and 0.006,respectively);(2)The percentage of CD19+ B-cells in AS patients was positively correlated with Bath Ankylosing Spondylitis Disease Activity Index(BASDAI)scores,Patient's Global Assessment(PGA)scores,total back pain scores and nocturnal back pain scores(r=0.270,0.255,0.251 and 0.266,P=0.029,0.039,0.042 and 0.031,respectively);(3)At week 6 and week 12,there were no statistical differences of the percentages of B-cells and subsets between etanercept group and placebo group of AS patients(P>0.05);the percentage of CD19+ B-cells in etanercept group was higher than that in healthy volunteers at week 12(t=3.320,P=0.003).Conclusions Misbalance is present in B-cells and some subsets in peripheral blood of active AS patients with peripheral joint involved.B-cells might play an important

  20. Random duality

    GUO TieXin; CHEN XinXiang


    The purpose of this paper is to provide a random duality theory for the further development of the theory of random conjugate spaces for random normed modules.First,the complicated stratification structure of a module over the algebra L(μ,K) frequently makes our investigations into random duality theory considerably different from the corresponding ones into classical duality theory,thus in this paper we have to first begin in overcoming several substantial obstacles to the study of stratification structure on random locally convex modules.Then,we give the representation theorem of weakly continuous canonical module homomorphisms,the theorem of existence of random Mackey structure,and the random bipolar theorem with respect to a regular random duality pair together with some important random compatible invariants.

  1. Random duality


    The purpose of this paper is to provide a random duality theory for the further development of the theory of random conjugate spaces for random normed modules. First, the complicated stratification structure of a module over the algebra L(μ, K) frequently makes our investigations into random duality theory considerably difierent from the corresponding ones into classical duality theory, thus in this paper we have to first begin in overcoming several substantial obstacles to the study of stratification structure on random locally convex modules. Then, we give the representation theorem of weakly continuous canonical module homomorphisms, the theorem of existence of random Mackey structure, and the random bipolar theorem with respect to a regular random duality pair together with some important random compatible invariants.

  2. Women’s perspectives and experiences on screening for osteoporosis (Risk-stratified Osteoporosis Strategy Evaluation, ROSE)

    Rothmann, Mette Juel; Huniche, Lotte; Ammentorp, Jette


    This study aimed to investigate women's perspectives and experiences with screening for osteoporosis. Focus groups and individual interviews were conducted. Three main themes emerged: knowledge about osteoporosis, psychological aspects of screening, and moral duty. Generally, screening was accepted...... due to life experiences, self-perceived risk, and the preventive nature of screening. PURPOSE: The risk-stratified osteoporosis strategy evaluation (ROSE) study is a randomized prospective population-based trial investigating the efficacy of a screening program to prevent fractures in women aged 65...... main themes: knowledge about osteoporosis, psychological aspects of screening, and moral duty. The women viewed the program in the context of their everyday life and life trajectories. Age, lifestyle, and knowledge about osteoporosis were important to how women ascribed meaning to the program, how...

  3. Largest sparse subgraphs of random graphs

    Fountoulakis, N.; Kang, R.J.; McDiarmid, C.J.H.; Nešetřil, J.; Győri, E.; Sali, A.


    For the Erd\\H{o}s-R\\'enyi random graph $G_{n,p}$, we consider the order of a largest vertex subset that induces a subgraph with average degree at most $t$. For the case when both $p$ and $t$ are fixed, this value is asymptotically almost surely concentrated on at most two explicitly given points. Th

  4. Leukocyte subsets and neutrophil function after short-term spaceflight

    Stowe, R. P.; Sams, C. F.; Mehta, S. K.; Kaur, I.; Jones, M. L.; Feeback, D. L.; Pierson, D. L.


    Changes in leukocyte subpopulations and function after spaceflight have been observed but the mechanisms underlying these changes are not well defined. This study investigated the effects of short-term spaceflight (8-15 days) on circulating leukocyte subsets, stress hormones, immunoglobulin levels, and neutrophil function. At landing, a 1.5-fold increase in neutrophils was observed compared with preflight values; lymphocytes were slightly decreased, whereas the results were variable for monocytes. No significant changes were observed in plasma levels of immunoglobulins, cortisol, or adrenocorticotropic hormone. In contrast, urinary epinephrine, norepinephrine, and cortisol were significantly elevated at landing. Band neutrophils were observed in 9 of 16 astronauts. Neutrophil chemotactic assays showed a 10-fold decrease in the optimal dose response after landing. Neutrophil adhesion to endothelial cells was increased both before and after spaceflight. At landing, the expression of MAC-1 was significantly decreased while L-selectin was significantly increased. These functional alterations may be of clinical significance on long-duration space missions.

  5. PRODH mutations and hyperprolinemia in a subset of schizophrenic patients.

    Jacquet, Hélène; Raux, Grégory; Thibaut, Florence; Hecketsweiler, Bernadette; Houy, Emmanuelle; Demilly, Caroline; Haouzir, Sadeq; Allio, Gabrielle; Fouldrin, Gael; Drouin, Valérie; Bou, Jacqueline; Petit, Michel; Campion, Dominique; Frébourg, Thierry


    The increased prevalence of schizophrenia among patients with the 22q11 interstitial deletion associated with DiGeorge syndrome has suggested the existence of a susceptibility gene for schizophrenia within the DiGeorge syndrome chromosomal region (DGCR) on 22q11. Screening for genomic rearrangements of 23 genes within or at the boundaries of the DGCR in 63 unrelated schizophrenic patients and 68 unaffected controls, using quantitative multiplex PCR of short fluorescent fragments (QMPSF), led us to identify, in a family including two schizophrenic subjects, a heterozygous deletion of the entire PRODH gene encoding proline dehydrogenase. This deletion was associated with hyperprolinemia in the schizophrenic patients. In addition, two heterozygous PRODH missense mutations (L441P and L289M), detected in 3 of 63 schizophrenic patients but in none among 68 controls, were also associated with increased plasma proline levels. Segregation analysis within the two families harboring respectively the PRODH deletion and the L441P mutation showed that the presence of a second PRODH nucleotide variation resulted in higher levels of prolinemia. In two unrelated patients suffering from severe type I hyperprolinemia with neurological manifestations, we identified a homozygous L441P PRODH mutation, associated with a heterozygous R453C substitution in one patient. These observations demonstrate that type I hyperprolinemia is present in a subset of schizophrenic patients, and suggest that the genetic determinism of type I hyperprolinemia is complex, the severity of hyperprolinemia depending on the nature and number of hits affecting the PRODH locus.

  6. Bayesian Subset Modeling for High-Dimensional Generalized Linear Models

    Liang, Faming


    This article presents a new prior setting for high-dimensional generalized linear models, which leads to a Bayesian subset regression (BSR) with the maximum a posteriori model approximately equivalent to the minimum extended Bayesian information criterion model. The consistency of the resulting posterior is established under mild conditions. Further, a variable screening procedure is proposed based on the marginal inclusion probability, which shares the same properties of sure screening and consistency with the existing sure independence screening (SIS) and iterative sure independence screening (ISIS) procedures. However, since the proposed procedure makes use of joint information from all predictors, it generally outperforms SIS and ISIS in real applications. This article also makes extensive comparisons of BSR with the popular penalized likelihood methods, including Lasso, elastic net, SIS, and ISIS. The numerical results indicate that BSR can generally outperform the penalized likelihood methods. The models selected by BSR tend to be sparser and, more importantly, of higher prediction ability. In addition, the performance of the penalized likelihood methods tends to deteriorate as the number of predictors increases, while this is not significant for BSR. Supplementary materials for this article are available online. © 2013 American Statistical Association.

  7. Zone diagrams in compact subsets of uniformly convex normed spaces

    Kopecká, Eva; Reich, Simeon


    A zone diagram is a relatively new concept which has emerged in computational geometry and is related to Voronoi diagrams. Formally, it is a fixed point of a certain mapping, and neither its uniqueness nor its existence are obvious in advance. It has been studied by several authors, starting with T. Asano, J. Matousek and T. Tokuyama, who considered the Euclidean plane with singleton sites, and proved the existence and uniqueness of zone diagrams there. In the present paper we prove the existence of zone diagrams with respect to finitely many pairwise disjoint compact sites contained in a compact and convex subset of a uniformly convex normed space. The proof is based on the Schauder fixed point theorem, the Curtis-Schori theorem regarding the Hilbert cube, and on recent results concerning the characterization of Voronoi cells as a collection of line segments and their geometric stability with respect to small changes of the corresponding sites. Along the way we obtain the continuity of the Dom mapping as wel...

  8. NK cell subset redistribution during the course of viral infections

    Enrico eLugli


    Full Text Available Natural killer (NK cells are important effectors of innate immunity that play a critical role in the control of human viral infections. Indeed, given their capability to directly recognize virally infected cells without the need of specific antigen presentation, NK cells are on the first line of defense against these invading pathogens. By establishing cellular networks with a variety of cell types such as dendritic cells, NK cells can also amplify anti-viral adaptive immune responses. In turn, viruses evolved and developed several mechanisms to evade NK cell-mediated immune activity. It has been reported that certain viral diseases, including human immunodeficiency virus-1 (HIV-1 as well as cytomegalovirus (CMV infections, are associated with a pathologic redistribution of NK cell subsets in the peripheral blood. In particular, it has been observed the expansion of unconventional CD56neg NK cells, whose effector functions are significantly impaired as compared to that of conventional CD56pos NK cells. In this review, we address the impact of chronic viral infections on the functional and phenotypic perturbations of human NK cell compartment.

  9. Alteration of peripheral blood lymphocyte subsets in acute pancreatitis

    Miroslawa Pietruczuk; Milena I Dabrowska; Urszula Wereszczynska-Siemiatkowska; Andrzej Dabrowski


    AIM: To evaluate peripheral blood lymphocyte subsets in patients with acute pancreatitis (AP).METHODS: Twenty patients with mild AP (M-AP) and 15 with severe AP (S-AP) were included in our study. Peripheral blood lymphocytes were examined at d 1-3, 5,10 and 30 by means of flow cytometry.RESULTS: A significant depletion of circulating lymphocytes was found in AP. In the early AP, the magnitude of depletion was similar for T- and B- lymphocytes. In the late course of S-AP, B-lymphocytes were much more depleted than T-lymphocytes. At d 10, strong shift in the CD7+/CD19+ ratio implicating predominance of Tover B-lymphocytes in S-AP was found. Among T-lymphocytes, the significant depletion of the CD4+ population was observed in M-AP and S-AP, while CD8+ cells were in the normal range. Lymphocytes were found to strongly express activation markers: CD69, CD25, CD28,CD38 and CD122. Serum interleukin-2 (IL-2), IL-4, IL-5,IL-10, interferon-γ (IFN-γ) and tumor necrosis factor-α(TNF-α) levels were significantly increased in both forms of AP. The magnitude of elevation of cytokines known to be produced by Th2 was much higher than cytokines produced by Th1 cells.CONCLUSION: AP in humans is characterized by significant reduction of peripheral blood T- and B-lymphocytes.

  10. Optimal Features Subset Selection and Classification for Iris Recognition

    Roy Kaushik


    Full Text Available Abstract The selection of the optimal features subset and the classification have become an important issue in the field of iris recognition. We propose a feature selection scheme based on the multiobjectives genetic algorithm (MOGA to improve the recognition accuracy and asymmetrical support vector machine for the classification of iris patterns. We also suggest a segmentation scheme based on the collarette area localization. The deterministic feature sequence is extracted from the iris images using the 1D log-Gabor wavelet technique, and the extracted feature sequence is used to train the support vector machine (SVM. The MOGA is applied to optimize the features sequence and to increase the overall performance based on the matching accuracy of the SVM. The parameters of SVM are optimized to improve the overall generalization performance, and the traditional SVM is modified to an asymmetrical SVM to treat the false accept and false reject cases differently and to handle the unbalanced data of a specific class with respect to the other classes. Our experimental results indicate that the performance of SVM as a classifier is better than the performance of the classifiers based on the feedforward neural network, the k-nearest neighbor, and the Hamming and the Mahalanobis distances. The proposed technique is computationally effective with recognition rates of 99.81% and 96.43% on CASIA and ICE datasets, respectively.

  11. Optimal Features Subset Selection and Classification for Iris Recognition

    Prabir Bhattacharya


    Full Text Available The selection of the optimal features subset and the classification have become an important issue in the field of iris recognition. We propose a feature selection scheme based on the multiobjectives genetic algorithm (MOGA to improve the recognition accuracy and asymmetrical support vector machine for the classification of iris patterns. We also suggest a segmentation scheme based on the collarette area localization. The deterministic feature sequence is extracted from the iris images using the 1D log-Gabor wavelet technique, and the extracted feature sequence is used to train the support vector machine (SVM. The MOGA is applied to optimize the features sequence and to increase the overall performance based on the matching accuracy of the SVM. The parameters of SVM are optimized to improve the overall generalization performance, and the traditional SVM is modified to an asymmetrical SVM to treat the false accept and false reject cases differently and to handle the unbalanced data of a specific class with respect to the other classes. Our experimental results indicate that the performance of SVM as a classifier is better than the performance of the classifiers based on the feedforward neural network, the k-nearest neighbor, and the Hamming and the Mahalanobis distances. The proposed technique is computationally effective with recognition rates of 99.81% and 96.43% on CASIA and ICE datasets, respectively.

  12. Immunophenotype of normal and myelomatous plasma cell subsets

    Nelly eRobillard


    Full Text Available Plasma-cells are essentially characterized by the co-expression of CD138 and CD38, which allows their identification in flow cytometry in bone marrow, peripheral blood or cell suspensions from tissues. These terminally differentiated B-cells may lose the expression of surface CD19 and that of CD20 while retaining CD27. When malignant, they can gain a number of other markers such as CD28, CD33, CD56 or CD117 and lose CD27. Moreover, since each plasma cell is only able to produce a single type of immunoglobulins, they display isotypic restriction and clonal malignant plasma cells can be further characterized by their homogeneous expression of either kappa or lambda light chains. In multiple myeloma (MM, such plasma cell clones produce the immunoglobulin identified in plasma as an abnormal peak. In the bone marrow where they essentially accumulate, these plasma cells may however display various immunophenotypes. The latter were explored in a two-way approach. Firstly the various subsets delineated by the selective or common expression of CD19 together with combined CD56/CD28 were explored in normal and MM bone marrow. Then other aberrant markers’ expression was investigated, i.e. CD20, CD27, CD33, CD56, CD117. These data were compared to literature information. They underline the vast heterogeneity of MM plasma cells possibly accounting for the various answers to therapy of MM patients.

  13. Transport Phenomena in Stratified Multi-Fluid Flow in the Presence and Absence of Gravity

    Chigier, Norman; Humphrey, William


    Experiments are being conducted to study the effects of buoyancy on planar density-stratified shear flows. A wind tunnel generates planar flows separated by an insulating splitter plate, with either flow heated, which emerge from a two-dimensional nozzle. The objective is to isolate and define the effect of gravity and buoyancy on a stratified shear layer. To this end, both stably and unstably stratified layers will be investigated. This paper reports on the results of temperature and velocity measurements across the nozzle exit plane and downstream along the nozzle center plane.

  14. A phase IIa randomized, double-blind trial of erlotinib in inhibiting epidermal growth factor receptor signaling in aberrant crypt foci of the colorectum.

    Gillen, Daniel L; Meyskens, Frank L; Morgan, Timothy R; Zell, Jason A; Carroll, Robert; Benya, Richard; Chen, Wen-Pin; Mo, Allen; Tucker, Chris; Bhattacharya, Asmita; Huang, Zhiliang; Arcilla, Myra; Wong, Vanessa; Chung, Jinah; Gonzalez, Rachel; Rodriguez, Luz Maria; Szabo, Eva; Rosenberg, Daniel W; Lipkin, Steven M


    Colorectal cancer progresses through multiple distinct stages that are potentially amenable to chemopreventative intervention. Epidermal growth factor receptor (EGFR) inhibitors are efficacious in advanced tumors including colorectal cancer. There is significant evidence that EGFR also plays important roles in colorectal cancer initiation, and that EGFR inhibitors block tumorigenesis. We performed a double-blind randomized clinical trial to test whether the EGFR inhibitor erlotinib given for up to 30 days had an acceptable safety and efficacy profile to reduce EGFR signaling biomarkers in colorectal aberrant crypt foci (ACF), a subset of which progress to colorectal cancer, and normal rectal tissue. A total of 45 patients were randomized to one of three erlotinib doses (25, 50, and 100 mg) with randomization stratified by nonsteroidal anti-inflammatory drug (NSAID) use. There were no unanticipated adverse events with erlotinib therapy. Erlotinib was detected in both normal rectal mucosa and ACFs. Colorectal ACF phosphorylated ERK (pERK), phosphorylated EGFR (pEGFR), and total EGFR signaling changes from baseline were modest and there was no dose response. Overall, this trial did not meet is primary efficacy endpoint. Colorectal EGFR signaling inhibition by erlotinib is therefore likely insufficient to merit further studies without additional prescreening stratification or potentially longer duration of use.

  15. A stratified two-stage sampling design for digital soil mapping in a Mediterranean basin

    Blaschek, Michael; Duttmann, Rainer


    The quality of environmental modelling results often depends on reliable soil information. In order to obtain soil data in an efficient manner, several sampling strategies are at hand depending on the level of prior knowledge and the overall objective of the planned survey. This study focuses on the collection of soil samples considering available continuous secondary information in an undulating, 16 km²-sized river catchment near Ussana in southern Sardinia (Italy). A design-based, stratified, two-stage sampling design has been applied aiming at the spatial prediction of soil property values at individual locations. The stratification based on quantiles from density functions of two land-surface parameters - topographic wetness index and potential incoming solar radiation - derived from a digital elevation model. Combined with four main geological units, the applied procedure led to 30 different classes in the given test site. Up to six polygons of each available class were selected randomly excluding those areas smaller than 1ha to avoid incorrect location of the points in the field. Further exclusion rules were applied before polygon selection masking out roads and buildings using a 20m buffer. The selection procedure was repeated ten times and the set of polygons with the best geographical spread were chosen. Finally, exact point locations were selected randomly from inside the chosen polygon features. A second selection based on the same stratification and following the same methodology (selecting one polygon instead of six) was made in order to create an appropriate validation set. Supplementary samples were obtained during a second survey focusing on polygons that have either not been considered during the first phase at all or were not adequately represented with respect to feature size. In total, both field campaigns produced an interpolation set of 156 samples and a validation set of 41 points. The selection of sample point locations has been done using

  16. Randomization in clinical trials in orthodontics: its significance in research design and methods to achieve it.

    Pandis, Nikolaos; Polychronopoulou, Argy; Eliades, Theodore


    Randomization is a key step in reducing selection bias during the treatment allocation phase in randomized clinical trials. The process of randomization follows specific steps, which include generation of the randomization list, allocation concealment, and implementation of randomization. The phenomenon in the dental and orthodontic literature of characterizing treatment allocation as random is frequent; however, often the randomization procedures followed are not appropriate. Randomization methods assign, at random, treatment to the trial arms without foreknowledge of allocation by either the participants or the investigators thus reducing selection bias. Randomization entails generation of random allocation, allocation concealment, and the actual methodology of implementing treatment allocation randomly and unpredictably. Most popular randomization methods include some form of restricted and/or stratified randomization. This article introduces the reasons, which make randomization an integral part of solid clinical trial methodology, and presents the main randomization schemes applicable to clinical trials in orthodontics.

  17. Reduction of exacerbations by the PDE4 inhibitor roflumilast - the importance of defining different subsets of patients with COPD

    Goehring Udo M


    Full Text Available Abstract Background As chronic obstructive pulmonary disease (COPD is a heterogeneous disease it is unlikely that all patients will benefit equally from a given therapy. Roflumilast, an oral, once-daily phosphodiesterase 4 inhibitor, has been shown to improve lung function in moderate and severe COPD but its effect on exacerbations in unselected populations was inconclusive. This led to the question of whether a responsive subset existed that could be investigated further. Methods The datasets of two previous replicate, randomized, double-blind, placebo-controlled, parallel-group studies (oral roflumilast 500 μg or placebo once daily for 52 weeks that were inconclusive regarding exacerbations were combined in a post-hoc, pooled analysis to determine whether roflumilast reduced exacerbations in a more precisely defined patient subset. Results The pooled analysis included 2686 randomized patients. Roflumilast significantly decreased exacerbations by 14.3% compared with placebo (p = 0.026. Features associated with this reduction were: presence of chronic bronchitis with or without emphysema (26.2% decrease, p = 0.001, presence of cough (20.9% decrease, p = 0.006, presence of sputum (17.8% decrease, p = 0.03, and concurrent use of inhaled corticosteroids (ICS; 18.8% decrease, p = 0.014. The incidence of adverse events was similar with roflumilast and placebo (81.5% vs 80.1%, but more patients in the roflumilast group had events assessed as likely or definitely related to the study drug (21.5% vs 8.3%. Conclusions This post-hoc, pooled analysis showed that roflumilast reduced exacerbation frequency in a subset of COPD patients whose characteristics included chronic bronchitis with/without concurrent ICS. These observations aided the design of subsequent phase 3 studies that prospectively confirmed the reduction in exacerbations with roflumilast treatment. Trials registration identifiers: NCT00076089 and NCT00430729.

  18. Phenotypic and functional distinctions between the TH2+ and JRA+ T cell subsets in man.

    Reinherz, E L; Strelkauskas, A J; O'Brien, C; Schlossman, S F


    Prior work has demonstrated the existence of distinct human peripheral blood T cell subsets by utilizing heterologous as well as autoimmune antisera. In the present study, the relationship between the TH2+ and JRA+ T cell subsets was examined. T cells were purified with Sephadex G-200 anti-F(ab)2' affinity chromatography and E-rosetting technique, and subsequently fractionated into TH2+ and TH2- subsets by utilizing indirect immunofluorescence on FACS. Approximately 40 to 45% of the TH2- subset was shown to be JRA+, whereas less than 5% of the TH2+ subset was JRA+. In reciprocal studies, T cells were fractionated into JRA+ and JRA- subsets and reacted with heterologous antisera with anti-TH2+ specificity and indirect immunofluorescence. FACS analysis demonstrated that the JRA+ population contained no TH2+ T cells. In contrast, the JRA- population contained TH2+ T cells and accounted for the entire TH2+ subset found in the unfractionated T cell population. Functional studies showed that the TH2+ subset, and not the JRA+ subset, contain the effector population for cell-mediated lympholysis. It is concluded that the TH2+ and JRA+ T cell subsets define distinct and different T cell populations in man.


    QIU Xiang


    Turbulence structures and turbulent Counter-Gradient Transport(CGT) properties in the stratified flows with a sharp temperature interface are investigated by experimental measurements using LIF and PIV, by LES and by correlation analysis.

  20. Development of a Curved, Stratified, In Vitro Model to Assess Ocular Biocompatibility: e96448

    Cameron K Postnikoff; Robert Pintwala; Sara Williams; Ann M Wright; Denise Hileeto; Maud B Gorbet


    .... Methods Immortalized human corneal epithelial cells were grown to confluency on curved cellulose filters for seven days, and were then differentiated and stratified using an air-liquid interface...

  1. (Metrically) quarter-stratifiable spaces and their applications in the theory of separately continuous functions

    Banakh, Taras


    We introduce and study (metrically) quarter-stratifiable spaces and then apply them to generalize Rudin and Kuratowski-Montgomery theorems about the Baire and Borel complexity of separately continuous functions.

  2. Stratified shear flow in an inclined duct: coherent structures and mixing

    Lefauve, Adrien; Partridge, Jamie; Dalziel, Stuart; Linden, Paul


    We present laboratory experiments on the exchange flow in an inclined square duct connecting two reservoirs at different densities. This system generates and maintains a stratified shear flow, which can be laminar, wavy or turbulent depending on the density difference and inclination angle. It is believed that the mean dissipation is set by the angle, and that high buoyancy Reynolds numbers (i.e. turbulent intensity) can be maintained, making this system suited for the study of continuously forced stratified turbulence. The talk will focus on the analysis of time-resolved, near-instantaneous 3D velocity and density data obtained by stereo particle image velocimetry (PIV) and laser induced fluorescence (LIF). This data allow for the visualisation of 3D coherent structures as well as turbulent mixing properties, which are key in understanding the dynamics of stratified turbulence. Supported by EPSRC Programme Grant EP/K034529/1 entitled "Mathematical Underpinnings of Stratified Turbulence".

  3. Mixture distribution measurement using laser induced breakdown spectroscopy in hydrogen direct injection stratified charge

    Shudo, Toshio [Applied Energy System Group, Division of Energy and Environmental Systems, Hokkaido University, N13 W8 Kita-Ward, Sapporo, Hokkaido 060-8628 (Japan); Oba, Shuji [Mazda Motor Corporation, Hiroshima 730-8670 (Japan)


    Reduction in cooling loss due to the heat transfer from burning gas to the combustion chamber wall is very important for improving the thermal efficiency in hydrogen engines. The previous research has shown that the direct injection stratified charge can be a technique to reduce the cooling loss and improve thermal efficiency in hydrogen combustion. For effective reductions in cooling loss by the stratified charge, it is very important to know the relation between the fuel injection conditions and mixture distribution. The current research employs the laser induced breakdown spectroscopy as a method to measure the hydrogen concentration distribution in the direct injection stratified charge. Measurement of instantaneous local equivalence ratio by the method clears the characteristics of mixture formation in hydrogen direct injection stratified charge. This research also tries to actively control the mixture distribution using a split fuel injection. (author)

  4. Molecular subsets in the gene expression signatures of scleroderma skin.

    Ausra Milano

    Full Text Available BACKGROUND: Scleroderma is a clinically heterogeneous disease with a complex phenotype. The disease is characterized by vascular dysfunction, tissue fibrosis, internal organ dysfunction, and immune dysfunction resulting in autoantibody production. METHODOLOGY AND FINDINGS: We analyzed the genome-wide patterns of gene expression with DNA microarrays in skin biopsies from distinct scleroderma subsets including 17 patients with systemic sclerosis (SSc with diffuse scleroderma (dSSc, 7 patients with SSc with limited scleroderma (lSSc, 3 patients with morphea, and 6 healthy controls. 61 skin biopsies were analyzed in a total of 75 microarray hybridizations. Analysis by hierarchical clustering demonstrates nearly identical patterns of gene expression in 17 out of 22 of the forearm and back skin pairs of SSc patients. Using this property of the gene expression, we selected a set of 'intrinsic' genes and analyzed the inherent data-driven groupings. Distinct patterns of gene expression separate patients with dSSc from those with lSSc and both are easily distinguished from normal controls. Our data show three distinct patient groups among the patients with dSSc and two groups among patients with lSSc. Each group can be distinguished by unique gene expression signatures indicative of proliferating cells, immune infiltrates and a fibrotic program. The intrinsic groups are statistically significant (p<0.001 and each has been mapped to clinical covariates of modified Rodnan skin score, interstitial lung disease, gastrointestinal involvement, digital ulcers, Raynaud's phenomenon and disease duration. We report a 177-gene signature that is associated with severity of skin disease in dSSc. CONCLUSIONS AND SIGNIFICANCE: Genome-wide gene expression profiling of skin biopsies demonstrates that the heterogeneity in scleroderma can be measured quantitatively with DNA microarrays. The diversity in gene expression demonstrates multiple distinct gene expression programs

  5. Economic viability of Stratified Medicine concepts : An investor perspective on drivers and conditions that favour using Stratified Medicine approaches in a cost-contained healthcare environment

    Fugel, Hans-Joerg; Nuijten, Mark; Postma, Maarten


    RATIONALE: Stratified Medicine (SM) is becoming a natural result of advances in biomedical science and a promising path for the innovation-based biopharmaceutical industry to create new investment opportunities. While the use of biomarkers to improve R&D efficiency and productivity is very much

  6. Supervised Feature Subset Selection based on Modified Fuzzy Relative Information Measure for classifier Cart



    Full Text Available Feature subset selection is an essential task in data mining. This paper presents a new method for dealing with supervised feature subset selection based on Modified Fuzzy Relative Information Measure (MFRIM. First, Discretization algorithm is applied to discretize numeric features to construct the membership functions of each fuzzy sets of a feature. Then the proposed MFRIM is applied to select the feature subset focusing on boundary samples. The proposed method can select feature subset with minimum number of features, which are relevant to get higher average classification accuracy for datasets. The experimental results with UCI datasets show that the proposed algorithm is effective and efficient in selecting subset with minimum number of features getting higher average classification accuracy than the consistency based feature subset selection method.

  7. Identification of Suitable Reference Genes for Peripheral Blood Mononuclear Cell Subset Studies in Multiple Sclerosis

    Oturai, D B; Søndergaard, H B; Börnsen, L;


    Quantitative real-time PCR (qPCR) involves the need of a proper standard for normalizing the gene expression data. Different studies have shown the validity of reference genes to vary greatly depending on tissue, cell subsets and experimental context. This study aimed at the identification...... of suitable reference genes for qPCR studies using different peripheral blood cell subsets (whole blood (WB) cells, peripheral blood mononuclear cells (PBMCs) and PBMC subsets (CD4(+) T cells, CD8(+) T cells, NK cells, monocytes, B cells and dendritic cells) from healthy controls (HC), patients with relapsing...... stable combination for analyses of cell subsets between HC and RRMS patients, while the combination of UBC and YWHAZ was superior for analysis of cell subsets between HC, RRMS and RRMS-IFN-β groups. GAPDH was generally unsuitable for blood cell subset studies in multiple sclerosis. In conclusion, we...

  8. A strategy that iteratively retains informative variables for selecting optimal variable subset in multivariate calibration.

    Yun, Yong-Huan; Wang, Wei-Ting; Tan, Min-Li; Liang, Yi-Zeng; Li, Hong-Dong; Cao, Dong-Sheng; Lu, Hong-Mei; Xu, Qing-Song


    Nowadays, with a high dimensionality of dataset, it faces a great challenge in the creation of effective methods which can select an optimal variables subset. In this study, a strategy that considers the possible interaction effect among variables through random combinations was proposed, called iteratively retaining informative variables (IRIV). Moreover, the variables are classified into four categories as strongly informative, weakly informative, uninformative and interfering variables. On this basis, IRIV retains both the strongly and weakly informative variables in every iterative round until no uninformative and interfering variables exist. Three datasets were employed to investigate the performance of IRIV coupled with partial least squares (PLS). The results show that IRIV is a good alternative for variable selection strategy when compared with three outstanding and frequently used variable selection methods such as genetic algorithm-PLS, Monte Carlo uninformative variable elimination by PLS (MC-UVE-PLS) and competitive adaptive reweighted sampling (CARS). The MATLAB source code of IRIV can be freely downloaded for academy research at the website:

  9. Contributions of cell subsets to cytokine production during normal and impaired wound healing.

    Mirza, Rita E; Koh, Timothy J


    The objective of this study was to determine the relative contributions of different cell subsets to the production of cytokines and growth factors during normal and impaired wound healing. Cells were isolated from wounds of non-diabetic and diabetic mice and separated by magnetic sorting into neutrophils/T cells/B cells (NTB cell subset), monocytes/macrophages (Mo/Mp subset) and non-leukocytic cells including keratinocyte/fibroblast/endothelial cells (KFE subset). On both per cell and total contribution bases, the Mo/Mp subset was the dominant producer of pro-inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-6 in both non-diabetic and diabetic mice and was a significant producer of vascular endothelial cell growth factor (VEGF)-A, insulin-like growth factor (IGF)-1 and transforming growth factor (TGF)-β1. The NTB subset was also a significant producer of TNF-α and IL-10 whereas the KFE subset contributed significant amounts of VEGF, IGF-1 and TGF-β1. Sustained production of pro-inflammatory cytokines and impaired production of healing-associated factors were evident in each subset in diabetic mice. These data will be useful for further experimental and modeling studies on the role of cell subsets in wound healing as well as for designing therapeutic strategies for improving healing.

  10. Lymphoid organ-resident dendritic cells exhibit unique transcriptional fingerprints based on subset and site.

    Kutlu G Elpek

    Full Text Available Lymphoid organ-resident DC subsets are thought to play unique roles in determining the fate of T cell responses. Recent studies focusing on a single lymphoid organ identified molecular pathways that are differentially operative in each DC subset and led to the assumption that a given DC subset would more or less exhibit the same genomic and functional profiles throughout the body. Whether the local milieu in different anatomical sites can also influence the transcriptome of DC subsets has remained largely unexplored. Here, we interrogated the transcriptional relationships between lymphoid organ-resident DC subsets from spleen, gut- and skin-draining lymph nodes, and thymus of C57BL/6 mice. For this purpose, major resident DC subsets including CD4 and CD8 DCs were sorted at high purity and gene expression profiles were compared using microarray analysis. This investigation revealed that lymphoid organ-resident DC subsets exhibit divergent genomic programs across lymphoid organs. Interestingly, we also found that transcriptional and biochemical properties of a given DC subset can differ between lymphoid organs for lymphoid organ-resident DC subsets, but not plasmacytoid DCs, suggesting that determinants of the tissue milieu program resident DCs for essential site-specific functions.

  11. Simulation model of stratified thermal energy storage tank using finite difference method

    Waluyo, Joko


    Stratified TES tank is normally used in the cogeneration plant. The stratified TES tanks are simple, low cost, and equal or superior in thermal performance. The advantage of TES tank is that it enables shifting of energy usage from off-peak demand for on-peak demand requirement. To increase energy utilization in a stratified TES tank, it is required to build a simulation model which capable to simulate the charging phenomenon in the stratified TES tank precisely. This paper is aimed to develop a novel model in addressing the aforementioned problem. The model incorporated chiller into the charging of stratified TES tank system in a closed system. The model was developed in one-dimensional type involve with heat transfer aspect. The model covers the main factors affect to degradation of temperature distribution namely conduction through the tank wall, conduction between cool and warm water, mixing effect on the initial flow of the charging as well as heat loss to surrounding. The simulation model is developed based on finite difference method utilizing buffer concept theory and solved in explicit method. Validation of the simulation model is carried out using observed data obtained from operating stratified TES tank in cogeneration plant. The temperature distribution of the model capable of representing S-curve pattern as well as simulating decreased charging temperature after reaching full condition. The coefficient of determination values between the observed data and model obtained higher than 0.88. Meaning that the model has capability in simulating the charging phenomenon in the stratified TES tank. The model is not only capable of generating temperature distribution but also can be enhanced for representing transient condition during the charging of stratified TES tank. This successful model can be addressed for solving the limitation temperature occurs in charging of the stratified TES tank with the absorption chiller. Further, the stratified TES tank can be

  12. An improved algorithm of image processing technique for film thickness measurement in a horizontal stratified gas-liquid two-phase flow

    Kuntoro, Hadiyan Yusuf, E-mail:; Majid, Akmal Irfan; Deendarlianto, E-mail: [Center for Energy Studies, Gadjah Mada University, Sekip K-1A Kampus UGM, Yogyakarta 55281 (Indonesia); Department of Mechanical and Industrial Engineering, Faculty of Engineering, Gadjah Mada University, Jalan Grafika 2, Yogyakarta 55281 (Indonesia); Hudaya, Akhmad Zidni; Dinaryanto, Okto [Department of Mechanical and Industrial Engineering, Faculty of Engineering, Gadjah Mada University, Jalan Grafika 2, Yogyakarta 55281 (Indonesia)


    Due to the importance of the two-phase flow researches for the industrial safety analysis, many researchers developed various methods and techniques to study the two-phase flow phenomena on the industrial cases, such as in the chemical, petroleum and nuclear industries cases. One of the developing methods and techniques is image processing technique. This technique is widely used in the two-phase flow researches due to the non-intrusive capability to process a lot of visualization data which are contain many complexities. Moreover, this technique allows to capture direct-visual information data of the flow which are difficult to be captured by other methods and techniques. The main objective of this paper is to present an improved algorithm of image processing technique from the preceding algorithm for the stratified flow cases. The present algorithm can measure the film thickness (h{sub L}) of stratified flow as well as the geometrical properties of the interfacial waves with lower processing time and random-access memory (RAM) usage than the preceding algorithm. Also, the measurement results are aimed to develop a high quality database of stratified flow which is scanty. In the present work, the measurement results had a satisfactory agreement with the previous works.

  13. An improved algorithm of image processing technique for film thickness measurement in a horizontal stratified gas-liquid two-phase flow

    Kuntoro, Hadiyan Yusuf; Hudaya, Akhmad Zidni; Dinaryanto, Okto; Majid, Akmal Irfan; Deendarlianto


    Due to the importance of the two-phase flow researches for the industrial safety analysis, many researchers developed various methods and techniques to study the two-phase flow phenomena on the industrial cases, such as in the chemical, petroleum and nuclear industries cases. One of the developing methods and techniques is image processing technique. This technique is widely used in the two-phase flow researches due to the non-intrusive capability to process a lot of visualization data which are contain many complexities. Moreover, this technique allows to capture direct-visual information data of the flow which are difficult to be captured by other methods and techniques. The main objective of this paper is to present an improved algorithm of image processing technique from the preceding algorithm for the stratified flow cases. The present algorithm can measure the film thickness (hL) of stratified flow as well as the geometrical properties of the interfacial waves with lower processing time and random-access memory (RAM) usage than the preceding algorithm. Also, the measurement results are aimed to develop a high quality database of stratified flow which is scanty. In the present work, the measurement results had a satisfactory agreement with the previous works.

  14. The effect of a high-protein, high-sodium diet on calcium and bone metabolism in postmenopausal women stratified by hormone replacement therapy use

    Harrington, M.; Bennett, T.; Jakobsen, Jette;


    randomly assigned to a diet high in protein ( 90 g/day) and sodium (180 mmol/day) ( calciuric diet) or a diet moderate in protein ( 70 g/day) and low in sodium ( 65 mmol/day) for 4 weeks followed by crossover to alternative dietary regimen for a further 4 weeks. The calciuric diet significantly (P......The objective of this study was to investigate the influence of a high-sodium, high-protein diet on bone metabolism in postmenopausal women ( aged 49 - 60 y) stratified by hormone replacement therapy (HRT) use. In a crossover trial, 18 women (n = 8 HRT users (+HRT) and n = 10 nonusers (-HRT)) were...

  15. The effect of a high-protein, high-sodium diet on calcium and bone metabolism in postmenopausal women stratified by hormone replacement therapy use

    Harrington, M.; Bennett, T.; Jakobsen, Jette


    randomly assigned to a diet high in protein ( 90 g/day) and sodium (180 mmol/day) ( calciuric diet) or a diet moderate in protein ( 70 g/day) and low in sodium ( 65 mmol/day) for 4 weeks followed by crossover to alternative dietary regimen for a further 4 weeks. The calciuric diet significantly (P......The objective of this study was to investigate the influence of a high-sodium, high-protein diet on bone metabolism in postmenopausal women ( aged 49 - 60 y) stratified by hormone replacement therapy (HRT) use. In a crossover trial, 18 women (n = 8 HRT users (+HRT) and n = 10 nonusers (-HRT)) were...

  16. Using parental profiles to predict membership in a subset of college students experiencing excessive alcohol consequences: findings from a longitudinal study.

    Varvil-Weld, Lindsey; Mallett, Kimberly A; Turrisi, Rob; Abar, Caitlin C


    Previous research identified a high-risk subset of college students experiencing a disproportionate number of alcohol-related consequences at the end of their first year. With the goal of identifying pre-college predictors of membership in this high-risk subset, the present study used a prospective design to identify latent profiles of student-reported maternal and paternal parenting styles and alcohol-specific behaviors and to determine whether these profiles were associated with membership in the high-risk consequences subset. A sample of randomly selected 370 incoming first-year students at a large public university reported on their mothers' and fathers' communication quality, monitoring, approval of alcohol use, and modeling of drinking behaviors and on consequences experienced across the first year of college. Students in the high-risk subset comprised 15.5% of the sample but accounted for almost half (46.6%) of the total consequences reported by the entire sample. Latent profile analyses identified four parental profiles: positive pro-alcohol, positive anti-alcohol, negative mother, and negative father. Logistic regression analyses revealed that students in the negative-father profile were at greatest odds of being in the high-risk consequences subset at a follow-up assessment 1 year later, even after drinking at baseline was controlled for. Students in the positive pro-alcohol profile also were at increased odds of being in the high-risk subset, although this association was attenuated after baseline drinking was controlled for. These findings have important implications for the improvement of existing parent- and individual-based college student drinking interventions designed to reduce alcohol-related consequences.

  17. Effect of Jiedu Lifeitang on T lymphocyte subsets and inflammatory factors in patients with viral pneumonia

    Han-Song Wang


    Objective:To observe the effect of Jiedu Lifeitang on T lymphocyte subsets and inflammatory factors in adults with viral pneumonia.Methods: A total of 104 cases with viral pneumonia randomly included in the study were divided into treatment group (54 cases) and control group (50 cases). The control group was given ribavirin based on oral medical, and the treatment group was given the Jiedu Lifeitang based on the control group. After treatment for 1 week, the changes of T lymphocyte subsets (CD3+,CD4+,CD8+,CD4+/CD8+) and inflammatory factors (IL-6, IL-8, CRP, TNF-α) between the two groups were observed.Results:Before treatment, the difference of CD3+, CD4+, CD8+ and CD4+/CD8+ in both groups was not statistically significant (P>0.05). After treatment, the levels of CD3+, CD4+, CD4+/CD8+ significantly increased and CD8+ significantly decreased in both groups (P<0.05), the levels of IL-6, IL-8, CRP, TNF-α significantly decreased in both groups (P<0.05). The levels of CD3+, CD4+ and CD4+/CD8+ in treatment group were (66.47±21.24)%, (36.74±12.05)% and (1.77±0.31), respectively, which were significantly higher than that in the control group after treatment, the levels of CD8+, IL-6, IL-8,CRP and TNF-α in treatment group were (20.09±8.08)%, (20.75±10.23) ng/mL, (8.37±4.45) ng/mL, (3.67±1.48) mg/L and (22.12±9.14) ng/L, respectively, which were significantly lower than that in the control group after treatment (P<0.05).Conclusions: Jiedu Lifeitang can regulate immune function and inhibit inflammatory reaction in adults with viral pneumonia.

  18. CD38 Expression in a Subset of Memory T Cells Is Independent of Cell Cycling as a Correlate of HIV Disease Progression

    Daniela Würsch


    Full Text Available In order to determine if the expression of the activation marker CD38 can correlate with HIV disease progression independently of cycling, we performed a cluster-based multivariate correlation analysis of total circulating CD4+ T cell counts and viral loads with frequencies of CD38 and Ki67 expression on CD4+ lymphocytes from patients with untreated HIV infection, stratified in maturation subpopulations, and subpopulation subsets defined by the expression of CXCR5, CXCR3, and CCR4. The frequencies of the activated phenotypes %CD38+ Ki67− and %CD38+ Ki67+ of the CXCR5− CXCR3− CCR4+ (“pre-Th2” central memory (TCM cell subset clustered together, comprising a significant negative correlate of total circulating CD4+ T cell counts and a positive correlate of viral load in multivariate analysis. Frequency of cycling-uncoupled CD38 expression in “pre-Th2” TCM cells was a negative correlate of total circulating CD4+ T cell counts in univariate analysis, which was not the case of their %CD38+ Ki67+. CXCR5+ CXCR3− CCR4−  TCM cells were underrepresented in patients, and their absolute counts correlated negatively with their %CD38+ Ki67− but not with their % CD38+ Ki67+. Our results may imply that CD38 expression either reflects or participates in pathogenic mechanisms of HIV disease independently of cell cycling.

  19. Upper Extremity Injured Workers Stratified by Current Work Status: An Examination of Health Characteristics, Work Limitations and Work Instability

    H Grant


    Full Text Available Background: Upper extremity injured workers are an under-studied population. A descriptive comparison of workers with shoulder, elbow and hand injuries reporting to a Canadian Workplace Safety and Insurance Board (WSIB clinic was undertaken.Objective: To determine if differences existed between injury groups stratified by current work status.Methods: All WSIB claimants reporting to our upper extremity clinic between 2003 and 2008 were approached to participate in this descriptive study. 314 working and 146 non-working WSIB claimants completed the Disabilities of the Arm, Shoulder and Hand questionnaire (DASH; Short Form health survey (SF36; Worker’s Limitations Questionnaire and the Work Instability Scale. Various parametric and non-parametric analyses were used to assess significant differences between groups on demographic, work and health related variables.Results: Hand, followed by the shoulder and elbow were the most common site of injury. Most non-workers listed their current injury as the reason for being off work, and attempted to return to work once since their injury occurrence. Non-workers and a subset of workers at high risk for work loss showed significantly worse mental functioning. Workers identified physical demands as the most frequent injury-related on the job limitation. 60% of current workers were listed as low risk for work loss on the Work Instability Scale.Conclusions: Poorer mental functioning, being female and sustaining a shoulder injury were risk factors for work instability. Our cohort of injured non-workers were unable to return to work due to their current injury, reinforcing the need to advocate for modified duties, shorter hours and a work environment where stress and injury recurrence is reduced. Future studies examining pre-injury depression as a risk factor for prolonged work absences are warranted.

  20. Arborification de Wikip\\'edia et analyse s\\'emantique explicite stratifi\\'ee - Wikipedia Arborification and Stratified Explicit Semantic Analysis

    Haralambous, Yannis


    We present an extension of the Explicit Semantic Analysis method by Gabrilovich and Markovitch. Using their semantic relatedness measure, we weight the Wikipedia categories graph. Then, we extract a minimal spanning tree, using Chu-Liu-Edmonds' algorithm. We define a notion of stratified tfidf where the stratas, for a given Wikipedia page and a given term, are the classical tfidf and categorical tfidfs of the term in the ancestor categories of the page (ancestors in the sense of the minimal spanning tree). Our method is based on this stratified tfidf, which adds extra weight to terms that "survive" when climbing up the category tree. We evaluate our method by a text classification on the WikiNews corpus: it increases precision by 18%. Finally, we provide hints for future research.

  1. Modified digital image correlation for balancing the influence of subset size choice

    Li, Bang-Jian; Wang, Quanbao; Duan, Deng-Ping; Chen, Ji-An


    A modified digital image correlation (DIC) method is presented to balance the influence of subset size choice, improve the calculation efficiency, and enhance the measurement accuracy. The relations between pixels and the central pixel are investigated. A relatively large subset size is selected in the method and all the pixels in a subset are treated nonuniformly. Weight is assigned to each pixel dependent on the significance of the pixel to track a subset from deformed images, and weights are computed by Butterworth function based on the distances between the pixels and the central pixel in the subset. Compared with classical DIC, the proposed method is a compromise between a small and a large subset. The choice of subset size for classical DIC is displaced by choice of parameters of Butterworth function in the presented method. The influence of subset size is reduced, and the calculation efficiency is improved with enhanced measurement accuracy by the modified method. Computer-simulated speckle patterns and real experiments are applied to verify the proposed method.


    刘长安; 管增伟; 孙武; 邵玉霞; 李卓; 贾廷珍


    Objective To study on the expression and clinical significance of T lymphocyte subset and NK cell activity (NKA) in patients with colorectal cancer. Methods Fifty-seven cancer patients and 33 healthy controls were enrolled in this study. T lymphocyte subset was measured by SAP technique and NKA by LDH release assay based on K562 cells, which served as target cells.

  3. A class of compact subsets for non-sober topological spaces

    Poncet, Paul


    We define a class of subsets of a topological space which coincides with the class of compact saturated subsets when the space is sober, and with enough good properties when the space is not sober. This class is introduced especially in view of applications to capacity theory.

  4. Phenotypic, ultra-structural and functional characterization of bovine peripheral blood dendritic cell subsets

    Dendritic cells (DC) are multifunctional cells that bridge the gap between innate and adaptive immune systems. In bovine, significant information is lacking on the precise identity and role of peripheral blood DC subsets. In this study, we identify and characterize bovine peripheral blood DC subsets...

  5. An ordered-subsets proximal preconditioned gradient algorithm for edge-preserving PET image reconstruction.

    Mehranian, Abolfazl; Rahmim, Arman; Ay, Mohammad Reza; Kotasidis, Fotis; Zaidi, Habib


    In iterative positron emission tomography (PET) image reconstruction, the statistical variability of the PET data precorrected for random coincidences or acquired in sufficiently high count rates can be properly approximated by a Gaussian distribution, which can lead to a penalized weighted least-squares (PWLS) cost function. In this study, the authors propose a proximal preconditioned gradient algorithm accelerated with ordered subsets (PPG-OS) for the optimization of the PWLS cost function and develop a framework to incorporate boundary side information into edge-preserving total variation (TV) and Huber regularizations. The PPG-OS algorithm is proposed to address two issues encountered in the optimization of PWLS function with edge-preserving regularizers. First, the second derivative of this function (Hessian matrix) is shift-variant and ill-conditioned due to the weighting matrix (which includes emission data, attenuation, and normalization correction factors) and the regularizer. As a result, the paraboloidal surrogate functions (used in the optimization transfer techniques) end up with high curvatures and gradient-based algorithms take smaller step-sizes toward the solution, leading to a slow convergence. In addition, preconditioners used to improve the condition number of the problem, and thus to speed up the convergence, would poorly act on the resulting ill-conditioned Hessian matrix. Second, the PWLS function with a nondifferentiable penalty such as TV is not amenable to optimization using gradient-based algorithms. To deal with these issues and also to enhance edge-preservation of the TV and Huber regularizers by incorporating adaptively or anatomically derived boundary side information, the authors followed a proximal splitting method. Thereby, the optimization of the PWLS function is split into a gradient descent step (upgraded by preconditioning, step size optimization, and ordered subsets) and a proximal mapping associated with boundary weighted TV

  6. Random Matrices

    Stephanov, M A; Wettig, T


    We review elementary properties of random matrices and discuss widely used mathematical methods for both hermitian and nonhermitian random matrix ensembles. Applications to a wide range of physics problems are summarized. This paper originally appeared as an article in the Wiley Encyclopedia of Electrical and Electronics Engineering.

  7. Innate-like functions of natural killer T cell subsets result from highly divergent gene programs.

    Engel, Isaac; Seumois, Grégory; Chavez, Lukas; Samaniego-Castruita, Daniela; White, Brandie; Chawla, Ashu; Mock, Dennis; Vijayanand, Pandurangan; Kronenberg, Mitchell


    Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of the differential expression of a few transcription factors and cell-surface molecules. Here we have analyzed purified populations of thymic NKT cell subsets at both the transcriptomic level and epigenomic level and by single-cell RNA sequencing. Our data indicated that despite their similar antigen specificity, the functional NKT cell subsets were highly divergent populations with many gene-expression and epigenetic differences. Therefore, the thymus 'imprints' distinct gene programs on subsets of innate-like NKT cells that probably impart differences in proliferative capacity, homing, and effector functions.

  8. Multi-Objective Feature Subset Selection using Non-dominated Sorting Genetic Algorithm

    A. Khan


    Full Text Available This paper presents an evolutionary algorithm based technique to solve multi-objective feature subset selection problem. The data used for classification contains large number of features called attributes. Some of these attributes are not relevant and needs to be eliminated. In classification procedure, each feature has an effect on the accuracy, cost and learning time of the classifier. So, there is a strong requirement to select a subset of the features before building the classifier. This proposed technique treats feature subset selection as multi-objective optimization problem. This research uses one of the latest multi-objective genetic algorithms (NSGA - II. The fitness value of a particular feature subset is measured by using ID3. The testing accuracy acquired is then assigned to the fitness value. This technique is tested on several datasets taken from the UCI machine repository. The experiments demonstrate the feasibility of using NSGA-II for feature subset selection.

  9. A Genetic Algorithm for Selection of Fixed-Size Subsets with Application to Design Problems

    Mark A. Wolters


    Full Text Available The R function kofnGA conducts a genetic algorithm search for the best subset of k items from a set of n alternatives, given an objective function that measures the quality of a subset. The function fills a gap in the presently available subset selection software, which typically searches over a range of subset sizes, restricts the types of objective functions considered, or does not include freely available code. The new function is demonstrated on two types of problem where a fixed-size subset search is desirable: design of environmental monitoring networks, and D-optimal design of experiments. Additionally, the performance is evaluated on a class of constructed test problems with a novel design that is interesting in its own right.

  10. T-lymphocyte subsets in West African children: impact of age, sex, and season

    Lisse, I M; Aaby, P; Whittle, H


    OBJECTIVE: There has been no reference material for T-lymphocyte subsets for normal children in developing countries. We therefore used T-lymphocyte subset determinations among children in three different studies in Guinea-Bissau to construct age-related reference material and to examine possible...... determinants of T-lymphocyte subset levels. METHODS: A total of 803 healthy West African children younger than 6 years were included in the three community studies of T-lymphocyte subsets among twins and singletons, after measles infection and after measles immunization. We used the immunoalkaline phosphatase...... method to determine T-lymphocyte subsets. RESULTS: We found differences by age, sex, and season, whereas there were no significant differences by birth order, twinning, or ethnic group. The CD4+ percentage declined from birth to age 2 years, at which time it started to increase to higher levels at age 4...

  11. Outcomes of the RESTOR-MV Trial (Randomized Evaluation of a Surgical Treatment for Off-Pump Repair of the Mitral Valve).

    Grossi, Eugene A; Patel, Nirav; Woo, Y Joseph; Goldberg, Judith D; Schwartz, Charles F; Subramanian, Valavanur; Feldman, Ted; Bourge, Robert; Baumgartner, Norbert; Genco, Christopher; Goldman, Scott; Zenati, Marco; Wolfe, J Alan; Mishra, Yugal K; Trehan, Naresh; Mittal, Sanjay; Shang, Shulian; Mortier, Todd J; Schweich, Cyril J


    we sought to determine whether patients with functional mitral regurgitation (FMR) would benefit from ventricular reshaping by the Coapsys device (Myocor, Inc., Maple Grove, Minnesota). FMR occurs when ventricular remodeling impairs valve function. Coapsys is a ventricular shape change device placed without cardiopulmonary bypass to reduce FMR. It compresses the mitral annulus and reshapes the ventricle. We hypothesized that Coapsys for FMR would improve clinical outcomes compared with standard therapies. RESTOR-MV (Randomized Evaluation of a Surgical Treatment for Off-Pump Repair of the Mitral Valve) was a randomized, prospective, multicenter study of patients with FMR and coronary disease with core laboratory analysis. After enrollment, patients were stratified to the standard indicated surgery: either coronary artery bypass graft alone or coronary artery bypass graft with mitral valve repair. In each stratum, randomization was to either control (indicated surgery) or treatment (coronary artery bypass graft with Coapsys ventricular reshaping). the study was terminated when the sponsor failed to secure ongoing funding; 165 patients were randomized. Control and Coapsys both produced decreases in left ventricular (LV) end-diastolic dimension and MR at 2 years (p stroke, myocardial infarction, and valve reoperation) was significantly greater with Coapsys at 2 years (85% vs. 71%) (hazard ratio: 0.372; 95% confidence interval: 0.185 to 0.749; adjusted log-rank test; p = 0.019). analysis of RESTOR-MV indicates that patients with FMR requiring revascularization treated with ventricular reshaping rather than standard surgery had improved survival and a significant decrease in major adverse outcomes. This trial validates the concept of the ventricular reshaping strategy in this subset of patients with heart failure. (Randomized Evaluation of a Surgical Treatment for Off-Pump Repair of the Mitral Valve [RESTOR-MV]; NCT00120276). Published by Elsevier Inc. All rights

  12. [Individualized, personalized and stratified medicine: a challenge for allergology in ENT?].

    Chaker, Adam M; Klimek, L


    Individualized, personalized or stratified medicine approaches offer emerging opportunities in the field of allergy and ENT. Avoidance of side effects, targeted therapy approaches and stratified prevention promise better outcomes and optimal results for patients. Conceptual incongruencies remain with regard to definitions and perceptions of "personalized medicine". Serious ethical considerations have to be taken into account. The development of pharmacogenomics, molecular phenotyping, genomic sequencing and other -omics opens the door to unique mechanistic therapeutic advances. The molecular allergology and recombinant diagnostics available are tools that offer substantial improved diagnostics for the benefit of allergic patients, e. g. in anaphylaxis and food allergy. For stratified therapeutic approaches, however, regulatory affairs will have to keep pace with medical and scientific discovery.

  13. A dynamic subgrid-scale model for the large eddy simulation of stratified flow

    刘宁宇; 陆夕云; 庄礼贤


    A new dynamic subgrid-scale (SGS) model, including subgrid turbulent stress and heat flux models for stratified shear flow is proposed by using Yoshizawa’ s eddy viscosity model as a base model. Based on our calculated results, the dynamic subgrid-scale model developed here is effective for the large eddy simulation (LES) of stratified turbulent channel flows. The new SGS model is then applied to the large eddy simulation of stratified turbulent channel flow under gravity to investigate the coupled shear and buoyancy effects on the near-wall turbulent statistics and the turbulent heat transfer at different Richardson numbers. The critical Richardson number predicted by the present calculation is in good agreement with the value of theoretical analysis.

  14. A dynamic subgrid-scale model for the large eddy simulation of stratified flow


    A new dynamic subgrid-scale (SGS) model, including subgrid turbulent stress and heat flux models for stratified shear flow is proposed by using Yoshizawa's eddy viscosity model as a base model. Based on our calculated results, the dynamic subgrid-scale model developed here is effective for the large eddy simulation (LES) of stratified turbulent channel flows. The new SGS model is then applied to the large eddy simulation of stratified turbulent channel flow under gravity to investigate the coupled shear and buoyancy effects on the near-wall turbulent statistics and the turbulent heat transfer at different Richardson numbers. The critical Richardson number predicted by the present calculation is in good agreement with the value of theoretical analysis.

  15. A criterion for the onset of slugging in horizontal stratified air-water countercurrent flow

    Chun, Moon-Hyun; Lee, Byung-Ryung; Kim, Yang-Seok [Korea Advanced Institute of Science and Technology, Taejon (Korea, Republic of)] [and others


    This paper presents an experimental and theoretical investigation of wave height and transition criterion from wavy to slug flow in horizontal air-water countercurrent stratified flow conditions. A theoretical formula for the wave height in a stratified wavy flow regime has been developed using the concept of total energy balance over a wave crest to consider the shear stress acting on the interface of two fluids. From the limiting condition of the formula for the wave height, a necessary criterion for transition from a stratified wavy flow to a slug flow has been derived. A series of experiments have been conducted changing the non-dimensional water depth and the flow rates of air in a horizontal pipe and a duct. Comparisons between the measured data and the predictions of the present theory show that the agreement is within {plus_minus}8%.

  16. Effect of turbulent fluctuations on the behaviour of fountains in stratified environments

    Freire, D.; Cabeza, C.; Pauletti, S.; Sarasúa, G.; Bove, I.; Usera, G.; Martí, A. C.


    The interaction between a turbulent fountain and its stratified environment was studied. A heavy fluid, cold water, was injected vertically upwards into a linearly stratified medium. The round heavy-fluid jet reaches a maximum height before it begins to fall due to the effect of gravity. Because of the effects of friction and mixing, the vertical momentum and density of the jet fluid decrease as it submerges to an intermediate height of zero buoyancy. At this point, the jet fluid spreads as a horizontal front, intruding into the stratified environment. The degree of fluctuation in the proximity of the injection point was studied under both unrestricted- and restricted-flow configurations at the injection, using two differently sized stainless-steel woven-wire screens at the injection port as flow-restricting means. Using visualization and velocimetry techniques, both maximum and spreading heights were found to decrease with increasing turbulence at the point of injection.

  17. Third Annual Open Meeting of the UK Pharmacogenomics and Stratified Medicine Network Conference.

    Bradshaw, Elizabeth H


    Third Annual Open Meeting of the UK Pharmacogenetics and Stratified Medicine Network 14 January 2015, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK The third Annual Open Meeting of the UK Pharmacogenetics and Stratified Medicine Network was held on 14 January 2015 in association with the Wellcome Trust on the Wellcome Trust Genome Campus at Hinxton, Cambridge, UK. In the morning, speakers from Cancer Research UK, the Medical Research Council, Genomics England, Innovate UK (formerly TSB) and the Department of Health described the current major projects they are funding. In the afternoon, speakers from various universities around the United Kingdom presented data on pharmacogenetics and stratified medicine research covering diverse disease areas including cancers, warfarin dosing, Gaucher disease and rheumatoid arthritis.

  18. Convective heat transport in stratified atmospheres at low and high Mach number

    Anders, Evan H


    Convection in astrophysical systems is stratified and often occurs at high Rayleigh number (Ra) and low Mach number (Ma). Here we study stratified convection in the context of plane-parallel, polytropically stratified atmospheres. We hold the density stratification ($n_{\\rho}$) and Prandtl number (Pr) constant while varying Ma and Ra to determine the behavior of the Nusselt number (Nu), which quantifies the efficiency of convective heat transport. As Ra increases and $\\text{Ma} \\rightarrow 1$, a scaling of Nu $\\propto$ Ra$^{0.45}$ is observed. As Ra increases to a regime where Ma $\\geq 1$, this scaling gives way to a weaker Nu $\\propto$ Ra$^{0.19}$. In the regime of Ma $\\ll 1$, a consistent Nu $\\propto$ Ra$^{0.31}$ is retrieved, reminiscent of the Nu $\\propto$ Ra$^{2/7}$ seen in Rayleigh-B\\'{e}nard convection.

  19. Prediction of Stratified Flow Temperature Profiles in a Fully Insulated Environment

    Ahmad S. Awad


    Full Text Available The aim of the study is to present an analytical model to predict the temperature profiles in thermal stratified environment. Thermal stratification is encountered in many situations. The flow of contaminants and hydrocarbons in environment often get stratified. The prediction of temperature profiles and flow characteristics are essential for HVAC applications, environment and energy management. The temperature profiles in the stratified region are successfully obtained, in terms of flow-operating functions. The analytical model agrees well with the published experimental data as well as the related closed-form solutions, which is helpful for HVAC applications. The model will be further developed and incorporated within a numerical model in order to investigate the flow field characteristics and establish correlations for a wide range of parameters.



    A modular numerical model was developed for simulating density-stratified flow in domains with irregular bottom topography. The model was designed for examining interactions between stratified flow and topography, e.g., tidally driven flow over two-dimensional sills or internal solitary waves propagating over a shoaling bed. The model was based on the non-hydrostatic vorticity-stream function equations for a continuously stratified fluid in a rotating frame. A self-adaptive grid was adopted in the vertical coordinate, the Alternative Direction Implicit (ADI) scheme was used for the time marching equations while the Poisson equation for stream-function was solved based on the Successive Over Relaxation (SOR) iteration with the Chebyshev acceleration. The numerical techniques were described and three applications of the model were presented.

  1. VH1-44 gene usage defines a subset of canine B-cell lymphomas associated with better patient survival.

    Chen, Hsiao-Wei; Small, George W; Motsinger-Reif, Alison; Suter, Steven E; Richards, Kristy L


    The use of specific immunoglobulin heavy chain variable region (VH) genes has been associated with increased patient survival in human B-cell lymphomas (hBCL). Given the similarity of human and canine BCL (cBCL) in morphology and clinical treatment, we examined the choice of VH in cBCL and determined whether VH gene selection was a distinct feature associated with survival time in dogs. VH gene selection and mutational status in 52 cBCL, including 29 diffuse large B-cell lymphomas (cDLBCL, the most common subtype of cBCL), were analyzed by comparison with the 80 published canine germline VH gene sequences. We further examined the prognostic impact of the subgroups defined by these features on canine survival. We found that VH1-44 was preferentially expressed in the majority of the 52 cBCLs (60%) as well as in the majority of the cDLBCL subset (59%). VH1-44 gene expression was associated with a statistically better overall survival (p=0.039) in cBCL patients, as well as in the cDLBCL subset of patients (p=0.038). These findings suggest that VH gene selection in cBCL is not random and may therefore have functional implications for cBCL lymphomagenesis, in addition to being a useful prognostic biomarker.

  2. Effect of Cytomegalovirus (CMV) and Ageing on T-Bet and Eomes Expression on T-Cell Subsets

    Hassouneh, Fakhri; Lopez-Sejas, Nelson; Campos, Carmen; Sanchez-Correa, Beatriz; Tarazona, Raquel; Pera, Alejandra; Solana, Rafael


    The differential impact of ageing and cytomegalovirus (CMV) latent infection on human T-cell subsets remains to some extent controversial. The purpose of this study was to analyse the expression of the transcription factors T-bet and Eomes and CD57 on CD4+, CD4hiCD8lo and CD8+ T-cell subsets in healthy individuals, stratified by age and CMV serostatus. The percentage of CD4+ T-cells expressing T-bet or Eomes was very low, in particular in CD4+ T-cells from young CMV-seronegative individuals, and were higher in CMV-seropositive older individuals, in both CD57− and CD57+ CD4+ T-cells. The study of the minor peripheral blood double-positive CD4hiCD8lo T-cells showed that the percentage of these T-cells expressing both Eomes and T-bet was higher compared to CD4+ T-cells. The percentage of CD4hiCD8lo T-cells expressing T-bet was also associated with CMV seropositivity and the coexpression of Eomes, T-bet and CD57 on CD4hiCD8lo T-cells was only observed in CMV-seropositive donors, supporting the hypothesis that these cells are mature effector memory cells. The percentage of T-cells expressing Eomes and T-bet was higher in CD8+ T-cells than in CD4+ T-cells. The percentages of CD8+ T-cells expressing Eomes and T-bet increased with age in CMV-seronegative and -seropositive individuals and the percentages of CD57− CD8+ and CD57+ CD8+ T-cells coexpressing both transcription factors were similar in the different groups studied. These results support that CMV chronic infection and/or ageing are associated to the expansion of highly differentiated CD4+, CD4hiCD8lo and CD8+ T-cells that differentially express T-bet and Eomes suggesting that the expression of these transcription factors is essential for the generation and development of an effector-memory and effector T lymphocytes involved in conferring protection against chronic CMV infection. PMID:28661443

  3. Stratified epithelium in prostatic adenocarcinoma: a mimic of high-grade prostatic intraepithelial neoplasia.

    Hameed, Omar; Humphrey, Peter A


    Typically glands of prostatic adenocarcinoma have a single cell lining, although stratification can be seen in invasive carcinomas with a cribriform architecture, including ductal carcinoma. The presence and diagnostic significance of stratified cells within non-cribriform carcinomatous prostatic glands has not been well addressed. The histomorphological features and immunohistochemical profile of cases of non-cribriform prostatic adenocarcinoma with stratified malignant glandular epithelium were analyzed. These cases were identified from needle biopsy cases from the consultation files of one of the authors and from a review of 150 consecutive in-house needle biopsy cases of prostatic adenocarcinoma. Immunohistochemistry was performed utilizing antibodies reactive against high molecular weight cytokeratin (34betaE12), p63 and alpha-methylacyl-coenzyme-A racemase (AMACR). A total of 8 cases were identified, including 2 from the 150 consecutive in-house cases (1.3%). In 4 cases, the focus with glands having stratified epithelium was the sole carcinomatous component in the biopsy, while such a component represented 5-30% of the invasive carcinoma seen elsewhere in the remaining cases. The main attribute in all these foci was the presence of glandular profiles lined by several layers of epithelial cells with cytological and architectural features resembling flat or tufted high-grade prostatic intraepithelial neoplasia, but lacking basal cells as confirmed by negative 34betaE12 and/or p63 immunostains in all cases. The AMACR staining profile of the stratified foci was variable, with 4 foci showing positivity, and 3 foci being negative, including two cases that displayed AMACR positivity in adjacent non-stratified prostatic adenocarcinoma. Prostatic adenocarcinoma with stratified malignant glandular epithelium can be identified in prostate needle biopsy samples harboring non-cribriform prostatic adenocarcinoma and resembles glands with high-grade prostatic

  4. The exact packing measure for a random re-ordering of the Cantor set



    The packing measure for a random re-ordering of the Cantor set, the packing dimension for the random set belonging to a sequence satisfying the Hausdorff and packing measures and packing measures for random subsets of R belonging to a regular sequence have been obtained.


    Zhao Caidi; Li Yongsheng; Zhou Shengfan


    This article proves that the random dynamical system generated by a two- dimensional incompressible non-Newtonian fluid with multiplicative noise has a global random attractor, which is a random compact set absorbing any bounded nonrandom subset of the phase space.

  6. Estimation of Finite Population Mean in Stratified Random Sampling Using Auxiliary Attribute(s under Non-Response

    Javid Shabbir


    Full Text Available In this paper we propose a combined exponential ratio type estimator of finite population mean utilizing information on the auxiliary attribute(s under non-response. Expressions for bias and MSE of the proposed estimator are derived up to first order of approximation. An empirical study is carried out to observe the performances of the estimators.

  7. Damage localization and quantification of composite stratified beam Structures using residual force method

    Behtani, A.; Bouazzouni, A.; Khatir, S.; Tiachacht, S.; Zhou, Y.-L.; Abdel Wahab, M.


    In this paper, the problem of using measured modal parameters to detect and locate damage in beam composite stratified structures with four layers of graphite/epoxy [0°/902°/0°] is investigated. A technique based on the residual force method is applied to composite stratified structure with different boundary conditions, the results of damage detection for several damage cases demonstrate that using residual force method as damage index, the damage location can be identified correctly and the damage extents can be estimated as well.

  8. Influence of DC-CIK in advanced colorectal cancer patients on T lymphocyte subsets and cytokines

    Rong Wang; Min Yi; Shi-Rong Yang; Li-Xia Chai; Mao Hua


    Objective:To explore the effects of dendritic cells (DC)-cytokine induced killer cells (CIK) treatment on T lymphocyte subsets and cytokines in patients with advanced colorectal cancer. Methods:A total of 84 cases patients with advanced colorectal cancer were divided into two groups according to random number table method, each 42 cases, both two groups were given FOLFOX scheme chemotherapy, on the basis, the observation group were given supplementary DC-CIK treatment, compared the T lymphocy te subgroup: CD3+, CD4+, CD8+, CD4+/CD8+, Th1 cytokines, interleukin-2 (IL-2) and interferon gamma-γ (FN-γ), Th2 cytokines:interleukin 6 (IL-6), interleukin 4 (IL-4) of the two groups before and after treatment. Results:Compared with before treatment, the CD3+, CD4+, CD8+, CD4+/CD8+, Th1 cytokines IL-2 and IFN-γin observation group were significantly higher than after treatment , the CD3+, CD4+, CD8+, CD4+/CD8+, Th1 cytokines IL-2 and IFN-γin control group were significantly lower than after treatment, and the differences were all statistically significant;The CD3+, CD4+, CD8+, CD4+/CD8+, Th1 cytokines IL-2 and IFN-γin observation group after treatment were significantly higher than those in control group after treatment with statistical difference;CD8+, Th2 cytokines IL-4, IL-6 in two groups had no statistical significance before and after treatment. Conclusion:Chemotherapy can cause the immune function restrained in patients with advanced colorectal cancer, and DC-CIK supplementary therapy can significantly improve the immune function, enhance the anti tumor immune responses.

  9. An information theoretic approach to select alternate subsets of predictors for data-driven hydrological models

    Taormina, R.; Galelli, S.; Karakaya, G.; Ahipasaoglu, S. D.


    This work investigates the uncertainty associated to the presence of multiple subsets of predictors yielding data-driven models with the same, or similar, predictive accuracy. To handle this uncertainty effectively, we introduce a novel input variable selection algorithm, called Wrapper for Quasi Equally Informative Subset Selection (W-QEISS), specifically conceived to identify all alternate subsets of predictors in a given dataset. The search process is based on a four-objective optimization problem that minimizes the number of selected predictors, maximizes the predictive accuracy of a data-driven model and optimizes two information theoretic metrics of relevance and redundancy, which guarantee that the selected subsets are highly informative and with little intra-subset similarity. The algorithm is first tested on two synthetic test problems and then demonstrated on a real-world streamflow prediction problem in the Yampa River catchment (US). Results show that complex hydro-meteorological datasets are characterized by a large number of alternate subsets of predictors, which provides useful insights on the underlying physical processes. Furthermore, the presence of multiple subsets of predictors-and associated models-helps find a better trade-off between different measures of predictive accuracy commonly adopted for hydrological modelling problems.

  10. Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodeling.

    Eyerich, Stefanie; Eyerich, Kilian; Pennino, Davide; Carbone, Teresa; Nasorri, Francesca; Pallotta, Sabatino; Cianfarani, Francesca; Odorisio, Teresa; Traidl-Hoffmann, Claudia; Behrendt, Heidrun; Durham, Stephen R; Schmidt-Weber, Carsten B; Cavani, Andrea


    Th subsets are defined according to their production of lineage-indicating cytokines and functions. In this study, we have identified a subset of human Th cells that infiltrates the epidermis in individuals with inflammatory skin disorders and is characterized by the secretion of IL-22 and TNF-alpha, but not IFN-gamma, IL-4, or IL-17. In analogy to the Th17 subset, cells with this cytokine profile have been named the Th22 subset. Th22 clones derived from patients with psoriasis were stable in culture and exhibited a transcriptome profile clearly separate from those of Th1, Th2, and Th17 cells; it included genes encoding proteins involved in tissue remodeling, such as FGFs, and chemokines involved in angiogenesis and fibrosis. Primary human keratinocytes exposed to Th22 supernatants expressed a transcriptome response profile that included genes involved in innate immune pathways and the induction and modulation of adaptive immunity. These proinflammatory Th22 responses were synergistically dependent on IL-22 and TNF-alpha. Furthermore, Th22 supernatants enhanced wound healing in an in vitro injury model, which was exclusively dependent on IL-22. In conclusion, the human Th22 subset may represent a separate T cell subset with a distinct identity with respect to gene expression and function, present within the epidermal layer in inflammatory skin diseases. Future strategies directed against the Th22 subset may be of value in chronic inflammatory skin disorders.

  11. Random thoughts

    ajansen; kwhitefoot; panteltje1; edprochak; sudhakar, the


    In reply to the news story “How to make a quantum random-number generator from a mobile phone” (16 May,, see also p5), which describes a way of delivering random numbers by counting the number of photons that impinge on each of the individual pixels in the camera of a Nokia N9 smartphone.

  12. Proper orthogonal decomposition of velocity gradient fields in a simulated stratified turbulent wake: analysis of vorticity and internal waves

    Gurka, R.; Diamessis, P.; Liberzon, A.


    The characterization of three-dimensional space and time-dependent coherent structures and internal waves in stratified environment is one of the most challenging tasks in geophysical fluid dynamics. Proper orthogonal decomposition (POD) is applied to 2-D slices of vorticity and horizontal divergence obtained from 3-D DNS of a stratified turbulent wake of a towed sphere at Re=5x103 and Fr=4. The numerical method employed solves the incompressible Navier-Stokes equations under the Boussinesq approximation. The temporal discretization consists of three fractional steps: an explicit advancement of the nonlinear terms, an implicit solution of the Poisson equation for the pseudo-pressure (which enforces incompressibility), and an implicit solution of the Helmholtz equation for the viscous terms (where boundary conditions are imposed). The computational domain is assumed to be periodic in the horizontal direction and non-periodic in the vertical direction. The 2-D slices are sampled along the stream-depth (Oxz), span-depth (Oyz) and stream-span planes (Oxy) for 231 times during the interval, Nt ∈ [12,35] (N is the stratification frequency). During this interval, internal wave radiation from the wake is most pronounced and the vorticity field in the wake undergoes distinct structural transitions. POD was chosen amongst the available statistical tools due to its advantage in characterization of simulated and experimentally measured velocity gradient fields. The computational procedure, applied to any random vector field, finds the most coherent feature from the given ensemble of field realizations. The decomposed empirical eigenfunctions could be referred to as "coherent structures", since they are highly correlated in an average sense with the flow field. In our analysis, we follow the computationally efficient method of 'snapshots' to find the POD eigenfunctions of the ensemble of vorticity field realizations. The results contains of the separate POD modes, along with

  13. Random Assignment of Schools to Groups in the Drug Resistance Strategies Rural Project: Some New Methodological Twists

    Pettigrew, Jonathan; Miller-Day, Michelle; Krieger, Janice L.; Zhou, Jiangxiu; Hecht, Michael L.


    Random assignment to groups is the foundation for scientifically rigorous clinical trials. But assignment is challenging in group randomized trials when only a few units (schools) are assigned to each condition. In the DRSR project, we assigned 39 rural Pennsylvania and Ohio schools to three conditions (rural, classic, control). But even with 13 schools per condition, achieving pretest equivalence on important variables is not guaranteed. We collected data on six important school-level variables: rurality, number of grades in the school, enrollment per grade, percent white, percent receiving free/assisted lunch, and test scores. Key to our procedure was the inclusion of school-level drug use data, available for a subset of the schools. Also, key was that we handled the partial data with modern missing data techniques. We chose to create one composite stratifying variable based on the seven school-level variables available. Principal components analysis with the seven variables yielded two factors, which were averaged to form the composite inflate-suppress (CIS) score which was the basis of stratification. The CIS score was broken into three strata within each state; schools were assigned at random to the three program conditions from within each stratum, within each state. Results showed that program group membership was unrelated to the CIS score, the two factors making up the CIS score, and the seven items making up the factors. Program group membership was not significantly related to pretest measures of drug use (alcohol, cigarettes, marijuana, chewing tobacco; smallest p>.15), thus verifying that pretest equivalence was achieved. PMID:23722619

  14. Feature subset selection based on mahalanobis distance: a statistical rough set method

    Sun Liang; Han Chongzhao


    In order to select effective feature subsets for pattern classification, a novel statistics rough set method is presented based on generalized attribute reduction. Unlike classical reduction approaches, the objects in universe of discourse are signs of training sample sets and values of attributes are taken as statistical parameters. The binary relation and discernibility matrix for the reduction are induced by distance function. Furthermore, based on the monotony of the distance function defined by Mahalanobis distance, the effective feature subsets are obtained as generalized attribute reducts. Experiment result shows that the classification performance can be improved by using the selected feature subsets.

  15. Short communication: Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the cecal tonsil of broiler chickens

    Chunyu Liu


    Full Text Available Aflatoxin B1 (AFB1 is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB1 on the cytokines expression and T-cell subsets in the cecal tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB1/kg feed and AFB1 group (0.6 mg pure AFB1/kg feed. At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+ by qRT-PCR and flow cytometry methods were assessed in the cecal tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the cecal tonsils of broiler chickens, suggesting that the cell-mediated immunity of cecal tonsils might be impaired in broilers.

  16. Short communication: Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the cecal tonsil of broiler chickens

    Liu, C.; Jiang, M.; Fang, J.; Peng, X.; Cui, H.


    Afatoxin B1 (AFB1) is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB1 on the cytokines expression and T-cell subsets in the cecal tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB1/kg feed) and AFB1 group (0.6 mg pure AFB1/kg feed). At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α) mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+) by qRT-PCR and flow cytometry methods were assessed in the cecal tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the cecal tonsils of broiler chickens, suggesting that the cell-mediated immunity of cecal tonsils might be impaired in broilers. (Author)

  17. Analysis of Wave Propagation in Stratified Structures Using Circuit Analogues, with Application to Electromagnetic Absorbers

    Sjoberg, Daniel


    This paper presents an overview of how circuit models can be used for analysing wave propagation in stratified structures. Relatively complex structures can be analysed using models which are accessible to undergraduate students. Homogeneous slabs are modelled as transmission lines, and thin sheets between the slabs are modelled as lumped…

  18. An Examination of Item Selection Rules by Stratified CAT Designs Integrated with Content Balancing Methods.

    Leung, Chi-Keung; Chang, Hua-Hua; Hau, Kit-Tai

    The multistage alpha-stratified computerized adaptive testing (CAT) design advocated a new philosophy of pool management and item selection using low discriminating items first. It has been demonstrated through simulation studies to be effective both in reducing item overlap rate and enhancing pool utilization with certain pool types. Based on…

  19. Experimental investigation on isothermal stratified flow mixing in a horizontal T-junction

    Isaev, Alexander; Kulenovic, Rudi; Laurien, Eckart [Stuttgart Univ. (Germany). Inst. fuer Kernenergetik und Energiesysteme (IKE)


    Turbulent and stratified flows can lead to thermal fatigue in piping systems of nuclear power plants (NPP). Such flows can be investigated in the University of Stuttgart Fluid-Structure-Interaction (FSI) facility with a T-Junction at thermal conditions with temperature differences of up to 255 K and at pressures of maximum 75 bars.

  20. Analysing stratified medicine business models and value systems: innovation-regulation interactions.

    Mittra, James; Tait, Joyce


    Stratified medicine offers both opportunities and challenges to the conventional business models that drive pharmaceutical R&D. Given the increasingly unsustainable blockbuster model of drug development, due in part to maturing product pipelines, alongside increasing demands from regulators, healthcare providers and patients for higher standards of safety, efficacy and cost-effectiveness of new therapies, stratified medicine promises a range of benefits to pharmaceutical and diagnostic firms as well as healthcare providers and patients. However, the transition from 'blockbusters' to what might now be termed 'niche-busters' will require the adoption of new, innovative business models, the identification of different and perhaps novel types of value along the R&D pathway, and a smarter approach to regulation to facilitate innovation in this area. In this paper we apply the Innogen Centre's interdisciplinary ALSIS methodology, which we have developed for the analysis of life science innovation systems in contexts where the value creation process is lengthy, expensive and highly uncertain, to this emerging field of stratified medicine. In doing so, we consider the complex collaboration, timing, coordination and regulatory interactions that shape business models, value chains and value systems relevant to stratified medicine. More specifically, we explore in some depth two convergence models for co-development of a therapy and diagnostic before market authorisation, highlighting the regulatory requirements and policy initiatives within the broader value system environment that have a key role in determining the probable success and sustainability of these models.

  1. Analysis of Wave Propagation in Stratified Structures Using Circuit Analogues, with Application to Electromagnetic Absorbers

    Sjoberg, Daniel


    This paper presents an overview of how circuit models can be used for analysing wave propagation in stratified structures. Relatively complex structures can be analysed using models which are accessible to undergraduate students. Homogeneous slabs are modelled as transmission lines, and thin sheets between the slabs are modelled as lumped…

  2. "Being" Black and Strategizing for Excellence in a Racially Stratified Academic Hierarchy

    O'Connor, Carla; Mueller, Jennifer; Lewis, R. L'Heureux; Rivas-Drake, Deborah; Rosenberg, Seneca


    This article reports the findings of an ethnographic study of Black identity and achievement in one predominantly White high school featuring a racially stratified academic hierarchy (RSAH). Foregrounding the experiences of three exceptionally achieving Black girls against those of other high-achieving but less stellar students, the study…

  3. Vertical dispersion of light inertial particles in stably stratified turbulence: The influence of the Basset force

    van Aartrijk, M.; Clercx, H.J.H.


    The dispersion of light inertial particles $\\rho_p/\\rho_f = \\mathcal{O}(1)$ in statistically stationary stably stratified turbulence is studied by means of direct numerical simulations. The light particle dispersion behavior is found to be comparable to that of heavy particles when displayed as a

  4. Stratified Flow in a Room with Displacement Ventilation and Wall-Mounted Air Terminal devices

    Nielsen, Peter V.

    This paper describes experiments with wall-mounted air terminal devices. The stratified flow in the room is analyzed, and the influence of stratification and the influence of room dimensions on the velocity level and on the length scale are proved. The velocity level in the occupied zone can be d...

  5. Experimental Validation of a Domestic Stratified Hot Water Tank Model in Modelica for Annual Performance Assessment

    Carmo, Carolina; Dumont, Olivier; Nielsen, Mads Pagh


    coupled with energy system solutions is limited. In this poster, a discretized model of a stratified tank developed in Modelica is presented. The physical phenoma to be considered are the thermal transfers by conduction and convection – stratification, heat loss to ambient, charging and discharging...

  6. Modified Limiting Equilibrium Method for Stability Analysis of Stratified Rock Slopes

    Rui Yong


    Full Text Available The stratified rock of Jurassic strata is widely distributed in Three Gorges Reservoir Region. The limit equilibrium method is generally utilized in the stability analysis of rock slope with single failure plane. However, the stratified rock slope cannot be accurately estimated by this method because of different bedding planes and their variable shear strength parameters. Based on the idealized model of rock slope with bedding planes, a modified limiting equilibrium method is presented to determine the potential sliding surface and the factor of safety for the stratified rock slope. In this method, the S-curve model is established to define the spatial variations of the shear strength parameters c and  φ of bedding plane and the tensile strength of rock mass. This method was applied in the stability evaluation of typical stratified rock slope in Three Gorges Reservoir Region, China. The result shows that the factor of safety of the case study is 0.973, the critical sliding surface for the potential slip surface appears at bedding plane C, and the tension-controlled failure occurs at 10.5 m to the slope face.

  7. Study on Reform of College English Stratified Teaching Based on School-Based Characteristics

    Yang, Liu


    Considering the status quo of college English teaching, we implement stratified teaching, which reflects the idea of stratification in terms of teaching objects, teaching management, teaching process and assessment and evaluation, makes each students get development to the greatest extent in interactive teaching practice of teaching and learning…

  8. Theoretical and experimental investigation into the explosive boiling potential of thermally stratified liquid-liquid systems

    Fabiano, B.; Kersten, R.J.A.; Opschoor, G.; Pastorino, R.


    The occurrence of a rapid phase transition, or so-called explosive boiling, when a cold volatile liquid comes into contact with a hot liquid or hot surface is a potential hazard in industry. This study was focused on the explosive boiling potential of thermally stratified liquid-liquid systems that

  9. Measures to assess the prognostic ability of the stratified Cox proportional hazards model

    (Tybjaerg-Hansen, A.) The Fibrinogen Studies Collaboration.The Copenhagen City Heart Study; Tybjærg-Hansen, Anne


    Many measures have been proposed to summarize the prognostic ability of the Cox proportional hazards (CPH) survival model, although none is universally accepted for general use. By contrast, little work has been done to summarize the prognostic ability of the stratified CPH model; such measures w...

  10. Developing spatially stratified N{sub 2}O emission factors for Europe

    Leip, Adrian, E-mail: [European Commission-Joint Research Centre, Institute for Environment and Sustainability, Ispra (Italy); Busto, Mirko [International Institute for Applied Systems Analysis, Laxenburg (Austria); Winiwarter, Wilfried [International Institute for Applied Systems Analysis, Laxenburg (Austria); AIT Austrian Institute of Technology, Vienna (Austria)


    We investigate the possibility to replace the - so-called - Tier 1 IPCC approach to estimate soil N{sub 2}O emissions with stratified emissions factors that take into account both N-input and the spatial variability of the environmental conditions within the countries of the European Union, using the DNDC-Europe model. Spatial variability in model simulations is high and corresponds to the variability reported in literature for field data. Our results indicate that (a) much of the observed variability in N{sub 2}O fluxes reflects the response of soils to external conditions, (b) it is likely that national inventories tend to overestimate the uncertainties in their estimated direct N{sub 2}O emissions from arable soils; (c) on average over Europe, the fertilizer-induced emissions (FIE) coincide with the IPCC factors, but they display large spatial variations. Therefore, at scales of individual countries or smaller, a stratified approach considering fertilizer type, soil characteristics and climatic parameters is preferable. - Highlights: > Stratified modelling improves estimates of direct N2O emissions from arable soils. > Large variability between strata (expressed as countries) becomes evident. > Fertilizer-induced emissions of N{sub 2}O differ by N input (manure or mineral fertilizer). > National inventories likely overestimate the uncertainties for direct N2O emissions - Model simulations suggest that stratified country-specific N{sub 2}O emission factors are useful to better describe the soil emission behaviour of the European Union countries.

  11. Spontaneous Formation of Surface Magnetic Structure from Large-scale Dynamo in Strongly-stratified Convection

    Masada, Youhei


    We report the first successful simulation of spontaneous formation of surface magnetic structures from a large-scale dynamo by strongly-stratified thermal convection in Cartesian geometry. The large-scale dynamo observed in our strongly-stratified model has physical properties similar to those in earlier weakly-stratified convective dynamo simulations, indicating that the $\\alpha^2$-type mechanism is responsible for it. Additionally to the large-scale dynamo, we find that large-scale structures of the vertical magnetic field are spontaneously formed in the convection zone surface only for the case of strongly-stratified atmosphere. The organization of the vertical magnetic field proceeds in the upper convection zone within tens of convective turn-over time and band-like bipolar structures are recurrently-appeared in the dynamo-saturated stage. We examine possibilities of several candidates as the origin of the surface magnetic structure formation, and then suggest the existence of an as-yet-unknown mechanism ...




    Let L be a meet continuous lattice. It is proved that the category Top of topological spaces can be embedded in the category of stratified L-topological spaces as a concretely both reflective and coreflective full subcategory if and only if L is a continuous lattice.

  13. Computational Fluid Dynamics model of stratified atmospheric boundary-layer flow

    Koblitz, Tilman; Bechmann, Andreas; Sogachev, Andrey;


    For wind resource assessment, the wind industry is increasingly relying on computational fluid dynamics models of the neutrally stratified surface-layer. So far, physical processes that are important to the whole atmospheric boundary-layer, such as the Coriolis effect, buoyancy forces and heat...

  14. Coherence theory of electromagnetic wave propagation through stratified N-layer media

    Hoenders, B.J.; Bertolotti, M.

    The theory of second-order coherence in connection with wave propagation through a stratified N-layer (SNL) medium is developed. Especially, the influence of the SNL medium on the propagation of the coherence generated by a given state of coherence at the entrance plane of the medium is considered.

  15. Seasonal cyclogenesis and the role of near-surface stratified layer in the Bay of Bengal

    Murty, V.S.N.; Sarma, M.S.S.; Tilvi, V.

    The role of the near-surface stratified layer developed due to the spread of low salinity waters under the influence of freshwater influx on the cyclogenesis over the Bay of Bengal is addressed. The seasonal variation of the Effective Oceanic Layer...

  16. Study of stratified dielectric slab medium structures using pseudo-spectral time domain (PSTD) algorithm

    Tong, M.S.; Lu, Y.; Chen, Y.


    A planar stratified dielectric slab medium, which is an interesting problem in optics and geophysics, is studied using a pseudo-spectral time-domain (PSTD) algorithm. Time domain electric fields and frequency domain propagation characteristics of both single and periodic dielectric slab...

  17. Randomization in substance abuse clinical trials

    Woolson Robert F


    Full Text Available Abstract Background A well designed randomized clinical trial rates as the highest level of evidence for a particular intervention's efficacy. Randomization, a fundamental feature of clinical trials design, is a process invoking the use of probability to assign treatment interventions to patients. In general, randomization techniques pursue the goal of providing objectivity to the assignment of treatments, while at the same time balancing for treatment assignment totals and covariate distributions. Numerous randomization techniques, each with varying properties of randomness and balance, are suggested in the statistical literature. This paper reviews common randomization techniques often used in substance abuse research and an application from a National Institute on Drug Abuse (NIDA-funded clinical trial in substance abuse is used to illustrate several choices an investigator faces when designing a clinical trial. Results Comparisons and contrasts of randomization schemes are provided with respect to deterministic and balancing properties. Specifically, Monte Carlo simulation is used to explore the balancing nature of randomization techniques for moderately sized clinical trials. Results demonstrate large treatment imbalance for complete randomization with less imbalance for the urn or adaptive scheme. The urn and adaptive randomization methods display smaller treatment imbalance as demonstrated by the low variability of treatment allocation imbalance. For all randomization schemes, covariate imbalance between treatment arms was small with little variation between adaptive schemes, stratified schemes and unstratified schemes given that sample sizes were moderate to large. Conclusion We develop this paper with the goal of reminding substance abuse researchers of the broad array of randomization options available for clinical trial designs. There may be too quick a tendency for substance abuse researchers to implement the fashionable urn

  18. An improved dynamic subgrid-scale model and its application to large eddy simulation of stratified channel flows

    ZHONG; Fengquan(仲峰泉); LIU; Nansheng(刘难生); LU; Xiyun(陆夕云); ZHUANG; Lixian(庄礼贤)


    In the present paper, a new dynamic subgrid-scale (SGS) model of turbulent stress and heat flux for stratified shear flow is proposed. Based on our calculated results of stratified channel flow, the dynamic subgrid-scale model developed in this paper is shown to be effective for large eddy simulation (LES) of stratified turbulent shear flows. The new SGS model is then applied to the LES of the stratified turbulent channel flow to investigate the coupled shear and buoyancy effects on the behavior of turbulent statistics, turbulent heat transfer and flow structures at different Richardson numbers.

  19. Nfil3 is required for the development of all innate lymphoid cell subsets.

    Seillet, Cyril; Rankin, Lucille C; Groom, Joanna R; Mielke, Lisa A; Tellier, Julie; Chopin, Michael; Huntington, Nicholas D; Belz, Gabrielle T; Carotta, Sebastian


    Innate lymphoid cell (ILC) populations protect against infection and are essential for lymphoid tissue formation and tissue remodeling after damage. Nfil3 is implicated in the function of adaptive immune lineages and NK cell development, but it is not yet known if Nfil3 regulates other innate lymphoid lineages. Here, we identify that Nfil3 is essential for the development of Peyer's patches and ILC2 and ILC3 subsets. Loss of Nfil3 selectively reduced Peyer's patch formation and was accompanied by impaired recruitment and distribution of lymphocytes within the patches. ILC subsets exhibited high Nfil3 expression and genetic deletion of Nfil3 severely compromised the development of all subsets. Subsequently, Nfil3(-/-) mice were highly susceptible to disease when challenged with inflammatory or infectious agents. Thus, we demonstrate that Nfil3 is a key regulator of the development of ILC subsets essential for immune protection in the lung and gut.

  20. Smoothness spaces of higher order on lower dimensional subsets of the Euclidean space

    Ihnatsyeva, Lizaveta


    We study Sobolev type spaces defined in terms of sharp maximal functions on Ahlfors regular subsets of the Euclidean space and the relation between these spaces and traces of classical Sobolev spaces.

  1. Differential control of Helios+/− Treg development by monocyte subsets through disparate inflammatory cytokines

    Zhong, Hui; Yazdanbakhsh, Karina


    Control of Helios+/− Treg subset development is mediated through distinct cytokines and monocyte subpopulations.CD16+ monocytes inhibit Helios+ Treg proliferation through IL-12, whereas CD16− monocytes suppress Helios− Treg development through TNF-α.


    Su Liaoyuan; Tian Hailin; Xu Yingdong; Geng Yongzhi


    Objective: To determine the NK activity of lymphocyte subsets and the effects of low dose radiation.Methods: Lymphocyte subsets were separated by monoclonal antibodies. The NK activity of each subset on tumor cells was detected by radioactive release method.Results: The results showed that besides NK cells, CD4,CD8 and B cells alone can kill tumor cells. But the cellkilling activity of NK cells appeared to be strongest.There was synergistic effect between CD4 and NK cells.The activity of mixed lymphocytes was more than that of only one subset. The effect of low dose radiation (LDR)on NK activity of panlymphocytes or NK cells was different. Conclusion: This paper demonstrated that NK activity of mononuclear cells was called "NK activity of lymphocytes", but it is not true. Only when NK cells were separated by monoclonal antibodies, its killer activity can be called "activity of NK cells".

  3. Tailored immune responses: novel effector helper T cell subsets in protective immunity.

    Ervin E Kara


    Full Text Available Differentiation of naïve CD4⁺ cells into functionally distinct effector helper T cell subsets, characterised by distinct "cytokine signatures," is a cardinal strategy employed by the mammalian immune system to efficiently deal with the rapidly evolving array of pathogenic microorganisms encountered by the host. Since the T(H1/T(H2 paradigm was first described by Mosmann and Coffman, research in the field of helper T cell biology has grown exponentially with seven functionally unique subsets having now been described. In this review, recent insights into the molecular mechanisms that govern differentiation and function of effector helper T cell subsets will be discussed in the context of microbial infections, with a focus on how these different helper T cell subsets orchestrate immune responses tailored to combat the nature of the pathogenic threat encountered.

  4. Tailored immune responses: novel effector helper T cell subsets in protective immunity.

    Kara, Ervin E; Comerford, Iain; Fenix, Kevin A; Bastow, Cameron R; Gregor, Carly E; McKenzie, Duncan R; McColl, Shaun R


    Differentiation of naïve CD4⁺ cells into functionally distinct effector helper T cell subsets, characterised by distinct "cytokine signatures," is a cardinal strategy employed by the mammalian immune system to efficiently deal with the rapidly evolving array of pathogenic microorganisms encountered by the host. Since the T(H)1/T(H)2 paradigm was first described by Mosmann and Coffman, research in the field of helper T cell biology has grown exponentially with seven functionally unique subsets having now been described. In this review, recent insights into the molecular mechanisms that govern differentiation and function of effector helper T cell subsets will be discussed in the context of microbial infections, with a focus on how these different helper T cell subsets orchestrate immune responses tailored to combat the nature of the pathogenic threat encountered.

  5. The cell biology of cross-presentation and the role of dendritic cell subsets.

    Lin, Ming-Lee; Zhan, Yifan; Villadangos, Jose A; Lew, Andrew M


    The cell biology of cross-presentation is reviewed regarding exogenous antigen uptake, antigen degradation and entry into the major histocompatibility complex class I pathway. Whereas cross-presentation is not associated with enhanced phagocytic ability, certain receptors may favour uptake for cross-presentation for example mannose receptor for soluble glycoproteins. Perhaps, the defining property of the cross-presenting cell is some specialization in host machinery for handling and transport of antigen across organelles. Both cytosolic and vacuolar pathways are discussed. Which dendritic cell (DC) subset is the cross-presenting cell is explored. Cross-presentation is found within the CD8(+) subset resident in lymphoid organs. The role of other DC subsets (especially the migratory CD8(-) DC) and the route of antigen delivery are also discussed. Further consideration is given to antigen transfer between DC subsets and differential presentation to naive vs memory T cells.

  6. Studying the Role for CD4+ T Cell Subsets in Human Lupus


    subsets in human lupus PRINCIPAL INVESTIGATOR: Insoo Kang, M.D. CONTRACTING...SUBTITLE 5a. CONTRACT NUMBER Studying the role for CD4+ T cell subsets in human lupus 5b. GRANT NUMBER W81XWH-10-1-0150 5c. PROGRAM ELEMENT NUMBER...Distribution Unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT We have investigated whether and how autoimmune complex (AIC) in SLE ( lupus ) can

  7. Characteristic changes and their significance of peripheral follicular helper T cell subsets in liver transplant patient

    Ke ZHANG


    Full Text Available Objective  To investigate the expression of follicular helper T (Tfh cell subsets in peripheral blood of patients with liver transplantation (LT and relevance to the prognosis. Methods  Eleven liver transplant patients with stable liver function were enrolled in this study. The frequencies of Tfh subsets were examined by flow cytometry. The frequencies and the level of alanine aminotransferase (ALT, aspartate transaminase (AST and total bilirubin (TBIL were monitored dynamically within one month after LT. Results  The frequency of CD4+CXCR5+CXCR3–CCR6– Tfh2 subset in peripheral blood increased significantly after LT as compared with those before transplantation (P<0.05. In addition, the frequency of CD4+CXCR5+CXCR3–CCR6+ Tfh17 subset in peripheral blood showed a trend of increase. The frequency of CD4+CXCR5+CXCR3+CCR6– Tfh1 subset, however, showed a downtrend, but no statistical difference was found. Conclusion  The subsets of Tfh2 and Tfh17 may be involved in the regulation of alloimmune response and play a role in maintaining liver function stability in liver transplant patients. DOI: 10.11855/j.issn.0577-7402.2016.10.08

  8. The correlation of lymphocyte subsets, natural killer cell, and Parkinson's disease: a meta-analysis.

    Jiang, Sen; Gao, Hua; Luo, Qin; Wang, Pengfei; Yang, Xinling


    The correlation between immunity and Parkinson's disease was presented in many papers, which also discussed lymphocyte and natural killer cell. But these studies have yielded inconsistent results. To systematically review the relationship between the lymphocyte subsets/natural killer cell and the risk of Parkinson's disease, we electronically searched the SpringerLink, Web of Science, Ebsco-medline with full text, Pubmed, Elsevier-ScienceDirect, Ovid-lww-oup, Wanfang Data for case-control trials on comparing the number of peripheral blood lymphocyte subsets and natural killer cell in Parkinson's patients and healthy controls. According to the Cochrane methods, the reviewers selected literature, extracted data, and assessed the quality. Then, a meta-analysis was performed using RevMan 5.2. Finally, 21 case-control trials including 943 cases of Parkinson's disease were fit into our data analysis. Meta-analysis showed that the decreased numbers of CD3+, CD4+ lymphocyte subsets and the increased number of natural killer cell were found in Parkinson's disease patients. In the intermediate and late stage of PD, CD8+ lymphocyte subsets had a significant decrement. However, the number of B lymphocyte subsets had no significant association with Parkinson's disease. The lymphocyte subsets and NK cell may be associated with the risk of Parkinson's disease.

  9. Turbulent Transport by Diffusive Stratified Shear Flows: From Local to Global Models. I. Numerical Simulations of a Stratified Plane Couette Flow

    Garaud, Pascale; Gagnier, Damien; Verhoeven, Jan


    Shear-induced turbulence could play a significant role in mixing momentum and chemical species in stellar radiation zones, as discussed by Zahn. In this paper we analyze the results of direct numerical simulations of stratified plane Couette flows, in the limit of rapid thermal diffusion, to measure the turbulent viscosity and the turbulent diffusivity of a passive tracer as a function of the local shear and the local stratification. We find that the stability criterion proposed by Zahn, namely that the product of the gradient Richardson number and the Prandtl number must be smaller than a critical values {(J\\Pr )}c for instability, adequately accounts for the transition to turbulence in the flow, with {(J\\Pr )}c≃ 0.007. This result recovers and confirms the prior findings of Prat et al. Zahn’s model for the turbulent diffusivity and viscosity, namely that the mixing coefficient should be proportional to the ratio of the thermal diffusivity to the gradient Richardson number, does not satisfactorily match our numerical data. It fails (as expected) in the limit of large stratification where the Richardson number exceeds the aforementioned threshold for instability, but it also fails in the limit of low stratification where the turbulent eddy scale becomes limited by the computational domain size. We propose a revised model for turbulent mixing by diffusive stratified shear instabilities that properly accounts for both limits, fits our data satisfactorily, and recovers Zahn’s model in the limit of large Reynolds numbers.

  10. Random matrices

    Mehta, Madan Lal


    Since the publication of Random Matrices (Academic Press, 1967) so many new results have emerged both in theory and in applications, that this edition is almost completely revised to reflect the developments. For example, the theory of matrices with quaternion elements was developed to compute certain multiple integrals, and the inverse scattering theory was used to derive asymptotic results. The discovery of Selberg's 1944 paper on a multiple integral also gave rise to hundreds of recent publications. This book presents a coherent and detailed analytical treatment of random matrices, leading

  11. Randomized metarounding



    The authors present a new technique for the design of approximation algorithms that can be viewed as a generalization of randomized rounding. They derive new or improved approximation guarantees for a class of generalized congestion problems such as multicast congestion, multiple TSP etc. Their main mathematical tool is a structural decomposition theorem related to the integrality gap of a relaxation.

  12. Circulating TFH subset distribution is strongly affected in lupus patients with an active disease.

    Carole Le Coz

    Full Text Available Follicular helper T cells (TFH represent a distinct subset of CD4(+ T cells specialized in providing help to B lymphocytes, which may play a central role in autoimmune diseases having a major B cell component such as systemic lupus erythematosus. Recently, TFH subsets that share common phenotypic and functional characteristics with TFH cells from germinal centers, have been described in the peripheral blood from healthy individuals. The aim of this study was to analyze the distribution of such populations in lupus patients. Circulating TFH cell subsets were defined by multicolor flow cytometry as TFH17 (CXCR3(-CCR6(+, TFH1 (CXCR3 (+ CCR6(- or TFH2 (CXCR3(-CCR6(- cells among CXCR5 (+ CD45RA(-CD4(+ T cells in the peripheral blood of 23 SLE patients and 23 sex and age-matched healthy controls. IL-21 receptor expression by B cells was analyzed by flow cytometry and the serum levels of IL-21 and Igs were determined by ELISA tests. We found that the TFH2 cell subset frequency is strongly and significantly increased in lupus patients with an active disease (SLEDAI score>8, while the TFH1 cell subset percentage is greatly decreased. The TFH2 and TFH1 cell subset frequency alteration is associated with the presence of high Ig levels and autoantibodies in patient's sera. Moreover, the TFH2 cell subset enhancement correlates with an increased frequency of double negative memory B cells (CD27(-IgD(-CD19(+ cells expressing the IL-21R. Finally, we found that IgE levels in lupus patients' sera correlate with disease activity and seem to be associated with high TFH2 cell subset frequency. In conclusion, our study describes for the first time the distribution of circulating TFH cell subsets in lupus patients. Interestingly, we found an increased frequency of TFH2 cells, which correlates with disease activity. Our results suggest that this subset might play a key role in lupus pathogenesis.

  13. Numerical modeling of mixing in large stably stratified enclosures using TRACMIX++

    Christensen, Jakob

    This PhD dissertation focuses on the numerical modeling of stably stratified large enclosures. In stably stratified volumes, the distribution of temperature, species concentration etc become essentially 1-D throughout most of the enclosure. When the fluid in an enclosure is stratified, wall boundary buoyant jets, forced buoyant jets (injection of fluid) and natural convection plumes become the primary sources of mixing. The time constants for the buoyant jets may be considered as much smaller than the time constant for the mixing of the stratified ambient fluid, provided the combined volume occupied by the buoyant jets is small compared to the volume of the enclosure. Therefore, fluid transport by the buoyant jets may be considered as occurring instantaneously. For this reason this work focuses on deriving a numerical method which is able to solve the 1-D vertical fluid conservation equations, as given in Peterson (1994). Starting with the Eulerian fluid conservation equations given in Peterson (1994), a set of Lagrangian fluid conservation equations were derived. Combining the Lagrangian approach with operator splitting such that the convective step and the diffusive step is separated renders a very efficient, accurate, and stable numerical method as it is shown in this text. Since the stratified flow field frequently exhibits very strong gradients or so-called fronts, the generation of these fronts has to be accurately detected and tracked by the numerical method. Flow in stably stratified large enclosure has typically been modeled in the past using 1- or 2-zone models. The present model is new in that it belongs to the K-zone models where the number of zones is arbitrarily large and depends on the complexity of the solution and the accuracy requirement set by the user. Because fronts are present in the flow field, a Lagrangian type numerical method is used. A Lagrangian method facilitates front tracking and prevents numerical diffusion from altering the shape of

  14. Flow and transport within a coastal aquifer adjacent to a stratified water body

    Oz, Imri; Yechieli, Yoseph; Eyal, Shalev; Gavrieli, Ittai; Gvirtzman, Haim


    The existence of a freshwater-saltwater interface and the circulation flow of saltwater beneath the interface is a well-known phenomenon found at coastal aquifers. This flow is a natural phenomenon that occurs due to density differences between fresh groundwater and the saltwater body. The goals of this research are to use analytical, numerical, and physical models in order to examine the configuration of the freshwater-saltwater interface and the density-driven flow patterns within a coastal aquifer adjacent to long-term stratified saltwater bodies (e.g. meromictic lake). Such hydrological systems are unique, as they consist of three different water types: the regional fresh groundwater, and low and high salinity brines forming the upper and lower water layers of the stratified water body, respectively. This research also aims to examine the influence of such stratification on hydrogeological processes within the coastal aquifer. The coastal aquifer adjacent to the Dead Sea, under its possible future meromictic conditions, serves as an ideal example to examine these processes. The results show that adjacent to a stratified saltwater body three interfaces between three different water bodies are formed, and that a complex flow system, controlled by the density differences, is created, where three circulation cells are developed. These results are significantly different from the classic circulation cell that is found adjacent to non-stratified water bodies (lakes or oceans). In order to obtain a more generalized insight into the groundwater behavior adjacent to a stratified water body, we used the numerical model to perform sensitivity analysis. The hydrological system was found be sensitive to three dimensionless parameters: dimensionless density (i.e. the relative density of the three water bodies'); dimensionless thickness (i.e. the ratio between the relative thickness of the upper layer and the whole thickness of the lake); and dimensionless flux. The results

  15. Stratifying Parkinson's Patients With STN-DBS Into High-Frequency or 60 Hz-Frequency Modulation Using a Computational Model.

    Khojandi, Anahita; Shylo, Oleg; Mannini, Lucia; Kopell, Brian H; Ramdhani, Ritesh A


    High frequency stimulation (HFS) of the subthalamic nucleus (STN) is a well-established therapy for Parkinson's disease (PD), particularly the cardinal motor symptoms and levodopa induced motor complications. Recent studies have suggested the possible role of 60 Hz stimulation in STN-deep brain stimulation (DBS) for patients with gait disorder. The objective of this study was to develop a computational model, which stratifies patients a priori based on symptomatology into different frequency settings (i.e., high frequency or 60 Hz). We retrospectively analyzed preoperative MDS-Unified Parkinson's Disease Rating Scale III scores (32 indicators) collected from 20 PD patients implanted with STN-DBS at Mount Sinai Medical Center on either 60 Hz stimulation (ten patients) or HFS (130-185 Hz) (ten patients) for an average of 12 months. Predictive models using the Random Forest classification algorithm were built to associate patient/disease characteristics at surgery to the stimulation frequency. These models were evaluated objectively using leave-one-out cross-validation approach. The computational models produced, stratified patients into 60 Hz or HFS (130-185 Hz) with 95% accuracy. The best models relied on two or three predictors out of the 32 analyzed for classification. Across all predictors, gait and rest tremor of the right hand were consistently the most important. Computational models were developed using preoperative clinical indicators in PD patients treated with STN-DBS. These models were able to accurately stratify PD patients into 60 Hz stimulation or HFS (130-185 Hz) groups a priori, offering a unique potential to enhance the utilization of this therapy based on clinical subtypes. © 2017 International Neuromodulation Society.

  16. Baroclinic Vortices in Rotating Stratified Shearing Flows: Cyclones, Anticyclones, and Zombie Vortices

    Hassanzadeh, Pedram

    Large coherent vortices are abundant in geophysical and astrophysical flows. They play significant roles in the Earth's oceans and atmosphere, the atmosphere of gas giants, such as Jupiter, and the protoplanetary disks around forming stars. These vortices are essentially three-dimensional (3D) and baroclinic, and their dynamics are strongly influenced by the rotation and density stratification of their environments. This work focuses on improving our understanding of the physics of 3D baroclinic vortices in rotating and continuously stratified flows using 3D spectral simulations of the Boussinesq equations, as well as simplified mathematical models. The first chapter discusses the big picture and summarizes the results of this work. In Chapter 2, we derive a relationship for the aspect ratio (i.e., vertical half-thickness over horizontal length scale) of steady and slowly-evolving baroclinic vortices in rotating stratified fluids. We show that the aspect ratio is a function of the Brunt-Vaisala frequencies within the vortex and outside the vortex, the Coriolis parameter, and the Rossby number of the vortex. This equation is basically the gradient-wind equation integrated over the vortex, and is significantly different from the previously proposed scaling laws that find the aspect ratio to be only a function of the properties of the background flow, and independent of the dynamics of the vortex. Our relation is valid for cyclones and anticyclones in either the cyclostrophic or geostrophic regimes; it works with vortices in Boussinesq fluids or ideal gases, and non-uniform background density gradient. The relation for the aspect ratio has many consequences for quasi-equilibrium vortices in rotating stratified flows. For example, cyclones must have interiors more stratified than the background flow (i.e., super-stratified), and weak anticyclones must have interiors less stratified than the background (i.e., sub-stratified). In addition, this equation is useful to

  17. Comparison of Subset-Based Local and Finite Element-Based Global Digital Image Correlation

    Pan, Bing


    Digital image correlation (DIC) techniques require an image matching algorithm to register the same physical points represented in different images. Subset-based local DIC and finite element-based (FE-based) global DIC are the two primary image matching methods that have been extensively investigated and regularly used in the field of experimental mechanics. Due to its straightforward implementation and high efficiency, subset-based local DIC has been used in almost all commercial DIC packages. However, it is argued by some researchers that FE-based global DIC offers better accuracy because of the enforced continuity between element nodes. We propose a detailed performance comparison between these different DIC algorithms both in terms of measurement accuracy and computational efficiency. Then, by measuring displacements of the same calculation points using the same calculation algorithms (e.g., correlation criterion, initial guess estimation, subpixel interpolation, optimization algorithm and convergence conditions) and identical calculation parameters (e.g., subset or element size), the performances of subset-based local DIC and two FE-based global DIC approaches are carefully compared in terms of measurement error and computational efficiency using both numerical tests and real experiments. A detailed examination of the experimental results reveals that, when subset (element) size is not very small and the local deformation within a subset (element) can be well approximated by the shape function used, standard subset-based local DIC approach not only provides better results in measured displacements, but also demonstrates much higher computation efficiency. However, several special merits of FE-based global DIC approaches are indicated.

  18. Random point sets and their diffraction

    Baake, Michael


    The diffraction of various random subsets of the integer lattice $\\mathbb{Z}^{d}$, such as the coin tossing and related systems, are well understood. Here, we go one important step beyond and consider random point sets in $\\mathbb{R}^{d}$. We present several systems with an effective stochastic interaction that still allow for explicit calculations of the autocorrelation and the diffraction measure. We concentrate on one-dimensional examples for illustrative purposes, and briefly indicate possible generalisations to higher dimensions. In particular, we discuss the stationary Poisson process in $\\mathbb{R}^{d}$ and the renewal process on the line. The latter permits a unified approach to a rather large class of one-dimensional structures, including random tilings. Moreover, we present some stationary point processes that are derived from the classical random matrix ensembles as introduced in the pioneering work of Dyson and Ginibre. Their re-consideration from the diffraction point of view improves the intuiti...

  19. Dynamics of a buoyant plume in a linearly stratified environment using simultaneous PIV-PLIF measurements

    Mirajkar, Harish N


    The presence of stratified layer in atmosphere and ocean leads to buoyant vertical motions, commonly referred to as plumes. It is important to study the mixing dynamics of a plume at a local scale in order to model their evolution and growth. Such a characterization requires measuring the velocity and density of the mixing fluids simultaneously. Here, we present the results of a buoyant plume propagating in a linearly stratified medium with a density difference of 0.5%, thus yielding a buoyancy frequency of N=0.15 s^{-1}. To understand the plume behaviour, statistics such as centerline and axial velocities along varying downstream locations, turbulent kinetic energy, Reynolds stress, and buoyancy flux were measured. The centerline velocity was found to decrease with increase in height. The Reynolds stress and buoyancy flux profiles showed the presence of a unstable layer and the mixing associated within that layer.

  20. Reconstruction of stratified steady water waves from pressure readings on the ocean bed

    Chen, Robin Ming


    Consider a two-dimensional stratified solitary wave propagating through a body of water that is bounded below by an impermeable ocean bed. In this work, we study how such a wave can be reconstructed from data consisting of the wave speed, upstream and downstream density profile, and the trace of the pressure on the bed. First, we prove that this data uniquely determines the wave, both in the (real) analytic and Sobolev regimes. Second, for waves that consist of multiple layers of constant density immiscible fluids, we provide an exact formula describing each of the interfaces in terms of the data. Finally, for continuously stratified fluids, we detail a reconstruction scheme based on approximation by layer-wise constant density flows.

  1. Numerical simulation of stratified shear flow using a higher order Taylor series expansion method

    Iwashige, Kengo; Ikeda, Takashi [Hitachi, Ltd. (Japan)


    A higher order Taylor series expansion method is applied to two-dimensional numerical simulation of stratified shear flow. In the present study, central difference scheme-like method is adopted for an even expansion order, and upwind difference scheme-like method is adopted for an odd order, and the expansion order is variable. To evaluate the effects of expansion order upon the numerical results, a stratified shear flow test in a rectangular channel (Reynolds number = 1.7x10{sup 4}) is carried out, and the numerical velocity and temperature fields are compared with experimental results measured by laser Doppler velocimetry thermocouples. The results confirm that the higher and odd order methods can simulate mean velocity distributions, root-mean-square velocity fluctuations, Reynolds stress, temperature distributions, and root-mean-square temperature fluctuations.

  2. Double criticality and the two-way Boussinesq equation in stratified shallow water hydrodynamics

    Bridges, Thomas J.; Ratliff, Daniel J.


    Double criticality and its nonlinear implications are considered for stratified N-layer shallow water flows with N = 1, 2, 3. Double criticality arises when the linearization of the steady problem about a uniform flow has a double zero eigenvalue. We find that there are two types of double criticality: non-semisimple (one eigenvector and one generalized eigenvector) and semi-simple (two independent eigenvectors). Using a multiple scales argument, dictated by the type of singularity, it is shown that the weakly nonlinear problem near double criticality is governed by a two-way Boussinesq equation (non-semisimple case) and a coupled Korteweg-de Vries equation (semisimple case). Parameter values and reduced equations are constructed for the examples of two-layer and three-layer stratified shallow water hydrodynamics.

  3. Large eddy simulation of turbulent statistical and transport properties in stably stratified flows

    Xiang QIU; Yong-xiang HUANG; Zhi-ming LU; Yu-lu LIU


    Three dimensional large eddy simulation (LES) is performed in the inves-tigation of stably stratified turbulence with a sharp thermal interface. Main results are focused on the turbulent characteristic scale, statistical properties, transport properties,and temporal and spatial evolution of the scalar field. Results show that the buoyancy scale increases first, and then goes to a certain constant value. The stronger the mean shear, the larger the buoyancy scale. The overturning scale increases with the flow, and the mean shear improves the overturning scale. The flatness factor of temperature de-parts from the Ganssian distribution in a fairly large region, and its statistical properties are clearly different from those of the velocity fluctuations in strong stratified cases. Tur-bulent mixing starts from small scale motions, and then extends to large scale motions.

  4. A 3D Spectral Anelastic Hydrodynamic Code for Shearing, Stratified Flows

    Barranco, J A; Barranco, Joseph A.; Marcus, Philip S.


    We have developed a three-dimensional (3D) spectral hydrodynamic code to study vortex dynamics in rotating, shearing, stratified systems (e.g. the atmosphere of gas giant planets, protoplanetary disks around newly forming protostars). The time-independent background state is stably stratified in the vertical direction and has a unidirectional linear shear flow aligned with one horizontal axis. Superposed on this background state is an unsteady, subsonic flow that is evolved with the Euler equations subject to the anelastic approximation to filter acoustic phenomena. A Fourier-Fourier basis in a set of quasi-Lagrangian coordinates that advect with the background shear is used for spectral expansions in the two horizontal directions. For the vertical direction, two different sets of basis functions have been implemented: (1) Chebyshev polynomials on a truncated, finite domain, and (2) rational Chebyshev functions on an infinite domain. Use of this latter set is equivalent to transforming the infinite domain to ...

  5. The Hand Burn Severity (HABS) score: A simple tool for stratifying severity of hand burns.

    Bache, Sarah E; Fitzgerald O'Connor, Edmund; Theodorakopoulou, Evgenia; Frew, Quentin; Philp, Bruce; Dziewulski, Peter


    Hand burns represent a unique challenge to the burns team due to the intricate structure and unrivalled functional importance of the hand. The initial assessment and prognosis relies on consideration of the specific site involved as well as depth of the burn. We created a simple severity score that could be used by referring non-specialists and researchers alike. The Hand Burn Severity (HABS) score stratifies hand burns according to severity with a numerical value of between 0 (no burn) and 18 (most severe) per hand. Three independent assessors scored the photographs of 121 burned hands of 106 adult and paediatric patients, demonstrating excellent inter-rater reliability (r=0.91, pburn depth alone. The HABS score is a simple to use tool to stratify severity at initial presentation of hand burns which will be useful when referring, and when reporting outcomes. Copyright © 2016 Elsevier Ltd and ISBI. All rights reserved.

  6. Nonlinear dynamics at the interface of two-layer stratified flows over pronounced obstacles

    Cabeza, C; Bove, I; Freire, D; Marti, Arturo C; Sarasua, L G; Usera, G; Montagne, R; Araújo, M


    The flow of a two--layer stratified fluid over an abrupt topographic obstacle, simulating relevant situations in oceanographic problems, is investigated numerically and experimentally in a simplified two--dimensional situation. Experimental results and numerical simulations are presented at low Froude numbers in a two-layer stratified flow and for two abrupt obstacles, semi--cylindrical and prismatic. We find four different regimes of the flow immediately past the obstacles: sub-critical (I), internal hydraulic jump (II), Kelvin-Helmholtz at the interface (III) and shedding of billows (IV). The critical condition for delimiting the experiments is obtained using the hydraulic theory. Moreover, the dependence of the critical Froude number on the geometry of the obstacle are investigated. The transition from regime III to regime IV is explained with a theoretical stability analysis. The results from the stability analysis are confirmed with the DPIV measurements. In regime (IV), when the velocity upstream is lar...

  7. Mixed Convection Flow along a Stretching Cylinder in a Thermally Stratified Medium

    Swati Mukhopadhyay


    Full Text Available An analysis for the axisymmetric laminar boundary layer mixed convection flow of a viscous and incompressible fluid towards a stretching cylinder immersed in a thermally stratified medium is presented in this paper. Similarity transformation is employed to convert the governing partial differential equations into highly nonlinear ordinary differential equations. Numerical solutions of these equations are obtained by a shooting method. It is found that the heat transfer rate at the surface is lower for flow in a thermally stratified medium compared to that of an unstratified medium. Moreover, both the skin friction coefficient and the heat transfer rate at the surface are larger for a cylinder compared to that for a flat plate.

  8. Laboratory Studies of the Stratified Rotating Flow Passing over an Isolated Obstacle

    高守亭; 平凡


    We study the flow of a density-stratified fluid passing over an isolated obstacle, using towing-tank experiments.Our special concern is the response of the flow with different Froude numbers passing over a three-dimensional obstacle. A series of experiments of the stratified rotating flow passing over an isolated obstacle was carried out with the towering-tank controlled by the similarity laws and dynamic non-dimension parameters. These experiments show that the Froude number is a very important parameter, and the lee wave and the eddy structure appear simultaneously under an appropriate conditions. The effect of rotation on the lee wave is mainly to change wave amplitude, particularly to restrain the development of the lee wave and to promote the formation of an eddy.

  9. Acceleration of raindrops formation due to tangling-clustering instability in turbulent stratified atmosphere

    Elperin, T; Krasovitov, B; Kulmala, M; Liberman, M; Rogachevskii, I; Zilitinkevich, S


    Condensation of water vapor on active cloud condensation nuclei produces micron-size water droplets. To form rain, they must grow rapidly into at least 50-100 micron-size droplets. Observations show that this process takes only 15-20 minutes. The unexplained physical mechanism of such fast growth, is crucial for understanding and modeling of rain, and known as "condensation-coalescence bottleneck in rain formation". We show that the recently discovered phenomenon of the tangling clustering instability of small droplets in temperature-stratified turbulence (Phys. Fluids 25, 085104, 2013) results in the formation of droplet clusters with drastically increased droplet number densities and strong five-orders-of-magnitude enhancement of the collision-coalescence rate inside the clusters. The mechanism of tangling clustering instability in the temperature-stratified turbulence is much more effective than the previously considered pure inertial clustering caused by the centrifugal effect of turbulent vortices. Our a...

  10. Numerical study of thermally stratified flows of a fluid overlying a highly porous material

    Antoniadis, Panagiotis D.; Papalexandris, Miltiadis V.


    In this talk we are concerned with thermally stratified flows in domains that contain a macroscopic interface between a highly porous material and a pure-fluid domain. Our study is based on the single-domain approach according to which the same set of governing equations is employed both inside the porous medium and in the pure-fluid domain. Also, the mathematical model that we employ treats the porous skeleton as a rigid solid that is in thermal non-equilibrium with the fluid. First, we present briefly the basic steps of the derivation of the mathematical model. Then, we present and discuss numerical results for both thermally stratified shear flows and natural convection. Our discussion focuses on the role of thermal stratification on the flows of interest and on the effect of thermal non-equilibrium between the solid matrix and the fluid inside the porous medium. This work is supported by the National Fund for Scientific Research (FNRS), Belgium.

  11. Simulations of surfactant-laden drops rising in a density-stratified medium

    Martin, David W.; Blanchette, François


    We present simulations of the effects of insoluble surfactant on drops rising in unstratified media and density-stratified media. We consider an oil drop rising in water, and we track surfactant concentration on the drop surface. We first consider a drop coated with insoluble surfactant rising through an unstratified ambient. The drop speed is computed for various Marangoni numbers, and inertial and surface diffusion effects are also examined. In the second setup, we compare clean and contaminated drops rising through a linear density-stratified fluid. The entrained buoyancy is computed for various density gradients, and the effects of inertia and Marangoni number are characterized. We find that the entrained fluid slows the drop in a manner comparable to a vertical shift in the ambient density gradient.

  12. Stratified polymer brushes from microcontact printing of polydopamine initiator on polymer brush surfaces.

    Wei, Qiangbing; Yu, Bo; Wang, Xiaolong; Zhou, Feng


    Stratified polymer brushes are fabricated using microcontact printing (μCP) of initiator integrated polydopamine (PDOPBr) on polymer brush surfaces and the following surface initiated atom transfer radical polymerization (SI-ATRP). It is found that the surface energy, chemically active groups, and the antifouling ability of the polymer brushes affect transfer efficiency and adhesive stability of the polydopamine film. The stickiness of the PDOPBr pattern on polymer brush surfaces is stable enough to perform continuous μCP and SI-ATRP to prepare stratified polymer brushes with a 3D topography, which have broad applications in cell and protein patterning, biosensors, and hybrid surfaces. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Stability boundaries and sufficient stability conditions for stably stratified, monotonic shear flows

    Hirota, Makoto, E-mail: [Institute of Fluid Science, Tohoku University, Sendai, Miyagi 980-8577 (Japan); Morrison, Philip J. [Department of Physics and Institute for Fusion Studies, University of Texas at Austin, Austin, TX 78712 (United States)


    Highlights: • New stability criteria of stably stratified shear flow are discovered. • Our criteria substantially improve the Howard–Miles criterion (1961). • Our criteria also generalize Rayleigh's inflection point theorem. • The novel approach we found is also efficient as a numerical approach. - Abstract: Linear stability of inviscid, parallel, and stably stratified shear flow is studied under the assumption of smooth strictly monotonic profiles of shear flow and density, so that the local Richardson number is positive everywhere. The marginally unstable modes are systematically found by solving a one-parameter family of regular Sturm–Liouville problems, which can determine the stability boundaries more efficiently than solving the Taylor–Goldstein equation directly. By arguing for the non-existence of a marginally unstable mode, we derive new sufficient conditions for stability, which generalize the Rayleigh–Fjørtoft criterion for unstratified shear flows.

  14. Doubly stratified mixed convection flow of Maxwell nanofluid with heat generation/absorption

    Abbasi, F.M., E-mail: [Department of Mathematics, Comsats Institute of Information Technology, Islamabad 44000 (Pakistan); Shehzad, S.A. [Department of Mathematics, Comsats Institute of Information Technology, Sahiwal 57000 (Pakistan); Hayat, T. [Department of Mathematics, Quaid-i-Azam University, 45320, Islamabad 44000 (Pakistan); NAAM Research Group, Department of Mathematics, Faculty of Science, King Abdulaziz University, Jeddah 21589 (Saudi Arabia); Ahmad, B. [NAAM Research Group, Department of Mathematics, Faculty of Science, King Abdulaziz University, Jeddah 21589 (Saudi Arabia)


    Magnetohydrodynamic (MHD) doubly stratified flow of Maxwell nanofluid in presence of mixed convection is analyzed in this article. Effects of thermophoresis, Brownian motion and heat generation/absorption are present. The flow is induced due to linear stretching of sheet. Mathematical formulation is made under boundary layer approach. Expressions of velocity, temperature and nanoparticles concentration are developed. The obtained results are plotted and discussed to examine the variations in temperature and nanoparticles concentration due to different physical parameters. Numerical computations are made to obtain the values of local Nusselt and Sherwood numbers. Impact of sundry parameters on the flow quantities is analyzed graphically. - Highlights: • Double stratified flow of Maxwell nanofluid with mixed convection is modeled. • Thermophoresis and Brownian motion effects are encountered. • Computations are made to obtain the solution expressions. • Numerical values of local Nusselt and Sherwood numbers are computed and examined.

  15. Variation of T cell subset during acute rejection after liver transplantation in rhesus monkeys

    Ran Jiang-hua; Liu Jing; Zhang Xi-bing; Zhang Sheng-ning; Wu Shu-yuan; Li Lai-bang; Li Wang; Li Li


    Abstract BACKGROUND: Looking for the early diagnosis of acute rejection indicators after liver transplantation can assess the risk after liver transplantation quickly and effectively, and T lymphocytes play the significant role in acute rejection. OBJECTIVE:To observe the relationship between acute rejection and variation of expression of T cel subset in blood after liver transplantation in rhesus monkey. METHODS: The sixteen liver transplant models in rhesus monkey which were constructed successfuly by the method of “double-cuff and one support tube” were divided into two groups randomly: experiment group (no treated by immunosuppressant in perioperative period) and control group (treated by immunosuppressant in perioperative period). Then the blood specimen and liver tissue respectively were colected at 6, 12, 24 and 72 hours after operation. The levels of alanine transferase, aspartate aminotransferase, and total bilirubin were detected with the fuly automatic biochemical analyser. The levels of CD4+/CD8+were tested by flow cytometry. The liver tissue in rhesus monkey after liver transplantation was detected by hematoxylin-eosin staining. The degree of acute rejection was evaluated by Banff Score System. RESULTS AND CONCLUSION: Acute rejection appeared in the experiment group at 12, 24, and 72 hours after liver transplantation. Levels of alanine transferase, aspartate aminotransferase, and total bilirubin were significantly higher in the experimental group than in the control group at 24 and 72 hours after transplantation (P < 0.05). The expression of CD4+/CD8+of the experiment group and control group began to rise at 6 hours after surgery, but the experiment group increased the most obvious. CD4+/CD8+ expression was significantly greater in the experimental group than in the control group at 24 and 72 hours after transplantation (P < 0.05). Morphological pathology was severer, and Banff score was higher in the experiment group than in the control group at

  16. Immunology of Kaschin- Beck Disease: Studies on lymphocyte subsets, humoral immunity and their relationship with selenium

    WANG Zhi-lun; ZHAI Jun-min; GUO Ru-ning; CHEN Jing-hong; CHEN Yan; ZHOU Yang; Jean Vanderpas


    Objective To study the humoral immunity status and distribution pattern of lymphocyte subgroups of peripheral blood mononuclear cell (PBMC) in patients with Kaschin - Beck Disease (KBD), and their relationship with erythrocyte selenium. Methods 23 X- ray diagnosed patients, 22 age- and sex- matched healthy children in KBD affected area (KAA), And 25 in KBD non- affected area (KNAA) were randomly selected. Immunohistochemistry with monoclonal antibodies anti - CD4, anti-CD8. anti - CD20 was conducted to analyze the lymphocyte subsets. Serum IgM, IgA, IgG, Complement C3 and C4 were assayed using rate nephelometry (Array 360 System, USA). The contents of erythrocyte selenium was determined by 2,3 - diaminonaphthalene fluorescence assay. Results CD4+ and CD8 + cells percentage in PBMCs and serum IgA were significantly lower in KAA than those in KNAA( P < 0.05). CD20 +percentage in KAA displayed a decreasing trend compared to KNAA, although not statistically significantly. No statistical differences were found in CD4/CD8 ratio, serum IgG, IgM, C3 and C4 levels. Erythrocyte selenium level in KAA still showed a pronounced decrease compared to that in KNAA. Correlation analysis showed that erythrocyte selenium contents had a strong association with the CD4 cell percentage ( r =0.625, P <0.05), and also a close relationship with serum IgA ( r =0.462, P <0.05). In addition, we detected a moderate correlation between the serum IgA and CD4 + percentage ( r = 0. 130, P > 0. 05). Conclusion Taken together, our resalts suggested that children in KAA had a comparably low cellular immunity level manifested by the marked depression of CD4 and CD8 cells percentage, and their humoral immunity status was also in a state of moderate immune suppression. Of this immune disorder in KBD patients, selenium deficiency probably played a critical role via affecting the distribution pattern of peripheral blood lymphocyte. Selenium-deficiency and immune impairment maybe both have

  17. Experimental study of temperature fluctuations in forced stably stratified turbulent flows

    Eidelman, A; Gluzman, Y; Kleeorin, N; Rogachevskii, I


    We study experimentally temperature fluctuations in stably stratified forced turbulence in air flow. In the experiments with an imposed vertical temperature gradient, the turbulence is produced by two oscillating grids located nearby the side walls of the chamber. Particle Image Velocimetry is used to determine the turbulent and mean velocity fields, and a specially designed temperature probe with sensitive thermocouples is employed to measure the temperature field. We found that the ratio [(\\ell_x \

  18. Experiments on the Microenvironment and Breathing of a Person in Isothermal and Stratified Surroundings

    Nielsen, Peter V.; Jensen, Rasmus Lund; Litewnicki, Michal;


    This study investigates the characteristics of human exhalation. Experiments are performed on a breathing thermal manikin in a test room. The manikin is heated, and an artificial lung is used to generate varying air flows with specific flow rates and temperatures for breathing. Smoke visualisation...... is used to show the formation, movement and disappearance of the exhalation jets from both nose and mouth. The exhalation of breathing without ventilation in the room, and with stratified surroundings (displacement ventilation) is analysed....

  19. Indicators of problems evaluated by parents and children stratified by birth weight

    RODRIGUES-PALUCCI,Claudia Mazzer; Loureiro,Sonia Regina


    Abstract Studies on the impact of birth weight on child development require the participation of children as informants. The objective of this study was to compare the indicators of behavioral problems and depression in a cohort of school-age children stratified by birth weight and to investigate possible associations between the indicators of behavioral problems, evaluated by the parents, and depression, evaluated by the children. A total of 665 children, aged 10-11 years, distributed into f...

  20. High diatom production and export in stratified waters - A potential negative feedback to global warming

    Kemp, Alan E. S.; Villareal, Tracy A.


    It is widely held that increased stratification and reduced vertical mixing in the ocean driven by global warming will promote the replacement of diatoms by smaller phytoplankton and lead to an overall decrease in productivity and carbon export. Here we present contrary evidence from a synergy of modern observations and palaeo-records that reveal high diatom production and export from stratified waters. Diatom adaptations to stratified waters include the ability to grow in low light conditions in deep chlorophyll maxima; vertical migrations between nutricline depths and the surface, and symbioses with N2-fixing cyanobacteria in diatom-diazotroph associations (DDA). These strategies foster the maintenance of seed populations that may then exploit mixing events induced by storms or eddies, but may also inherently promote blooms. Recent oceanographic observations in the subtropical gyres, at increasingly high temporal and spatial resolutions, have monitored short-lived but often substantial blooms and export of stratified-adapted diatoms including rhizosolenids and the diazotroph-associated Hemiaulus hauckii. Aggregate formation by such diatoms is common and promotes rapid settling thereby minimizing water column remineralization and optimizing carbon flux. Convergence zones associated with oceanic fronts or mesoscale features may also generate substantial flux of stratified-adapted diatom species. Conventional oceanographic observing strategies and sampling techniques under-represent such activity due to the lack of adequate capability to sample the large sized diatoms and colonies involved, the subsurface location of many of these blooms, their common development in thin global warming. However, the key genera involved in such potential feedbacks are underrepresented in both laboratory and field studies and are poorly represented in models. Our findings suggest that a reappraisal is necessary of the way diatoms are represented as plankton functional types (PFTs) in

  1. A Model for Predicting Holdup and Pressure Drop in Gas-Liquid Stratified Flow


    The time-dependent liquid film thickness and pressure drop were measured by using parallel-wire conductance probes and capacitance differential-preesure transducers. Applying the eddy viscosity theory and an appropriate correlation of interfacial sear stress,a new two-dimensional separated model of holdup and pressure drop of turbulent/turbulent gas-liquid stratified flow was presented. Prediction results agreed well with experimental data.

  2. Recent advances in understanding the origin of magnetic flux concentrations in strongly stratified turbulence

    Brandenburg, Axel; Kleeorin, Nathan


    In the presence of strong density stratification, hydromagnetic turbulence attains qualitatively new properties: the formation of magnetic flux concentrations. We review here the theoretical foundations of this mechanism in terms of what is now called the negative effective magnetic pressure instability. We also present direct numerical simulations of forced turbulence in strongly stratified layers and discuss the qualitative and quantitative similarities with corresponding mean-field simulations. Finally, the relevance to sunspot formation is discussed.

  3. An immersed interface method for two-dimensional modelling of stratified flow in pipes

    Berthelsen, Petter Andreas


    This thesis deals with the construction of a numerical method for solving two-dimensional elliptic interface problems, such as fully developed stratified flow in pipes. Interface problems are characterized by its non-smooth and often discontinuous behaviour along a sharp boundary separating the fluids or other materials. Classical numerical schemes are not suitable for these problems due to the irregular geometry of the interface. Standard finite difference discretization across the interface...

  4. Propagation of Long-Wavelength Nonlinear Slow Sausage Waves in Stratified Magnetic Flux Tubes

    Barbulescu, M.; Erdélyi, R.


    The propagation of nonlinear, long-wavelength, slow sausage waves in an expanding magnetic flux tube, embedded in a non-magnetic stratified environment, is discussed. The governing equation for surface waves, which is akin to the Leibovich-Roberts equation, is derived using the method of multiple scales. The solitary wave solution of the equation is obtained numerically. The results obtained are illustrative of a solitary wave whose properties are highly dependent on the degree of stratification.

  5. A Model of Turbulent-Laminar Gas-Liquid Stratified Flow


    The time-dependent liquid film thickness and pressure drop are measured by using parallel-wire conduc tance probes and capacitance differential-pressure transducer. A mathematical model with iterative procedure to calculate holdup and pressure drop in horizontal and inclined gas-liquid stratified flow is developed. The predictions agree well with over a hundred experimental data in 0.024 and 0.04 m diameter pipelines.

  6. The Near Wake of Bluff Bodies in Stratified Fluids and the Emergence of Late Wake Characteristics


    Kundu and Ira Cohen. Fluid Mechanics , Third Edition. Elsevier Inc., 2004. [27] S. Lee. A numerical study of the unsteady wake behind a sphere in a...found in the stratified fluid flows is the existence of the standing lee wave, which is a laminar mechanism . Above a Reynolds number of 2000, it is...computational cost to both model the proper fluid mechanics on the sphere and reproduce the far-wake is prohibitively expensive with current

  7. 3D elastic inversion of vertical seismic profiles in horizontally stratified media; Inversion elastique 3D de profils sismiques verticaux en milieux stratifies horizontalement

    Petit, J.L.


    This thesis is devoted to the inversion of VSP (vertical seismic profile) seismic data in order to determine the elastic properties of horizontally stratified media. The VSP records are computed using the full wave elastic modelling in isotropic and transversely isotropic media using Hankel transform, a finite difference scheme and an inverse Hankel transform algorithm, and the propagation equations are determined and numerically solved; the importance of considering a 3D wave propagation model instead of a 1 D one is emphasized. The theoretical VSP inverse problem is then considered, with the seismic waveform inversion set as a least-squares problem, consisting in recovering the distribution of physical parameters which minimize the misfit between calculated and observed VSP. The corresponding problem requires the knowledge of the source function

  8. Circulating T helper and T regulatory subsets in untreated early rheumatoid arthritis and healthy control subjects.

    Pandya, Jayesh M; Lundell, Anna-Carin; Hallström, Magnus; Andersson, Kerstin; Nordström, Inger; Rudin, Anna


    The pathogenic role and frequency of T cell subtypes in early rheumatoid arthritis are still unclear. We therefore performed a comprehensive analysis of the circulating T cell subtype pattern in patients with untreated early rheumatoid arthritis compared to healthy control subjects. Peripheral blood mononuclear cells were obtained from 26 patients with untreated early rheumatoid arthritis and from with 18 age- and sex-matched healthy control subjects. T helper cell types Th0, Th1, Th2, Th17, and Th1/17 and nonclassic T helper subsets were defined by flow cytometry based on the expression of chemokine receptors CCR4, CCR6, and CXCR3. Regulatory T cells were defined by expression of CD25(+) CD127(low) and also FOXP3 CXCR5(+) cells among regulatory and nonregulatory T cells were defined as T follicular regulatory and T follicular helper cells, respectively. The phenotype of T cell subsets was confirmed by transcription factor and cytokine secretion analyses. Multivariate discriminant analysis showed that patients with untreated early rheumatoid arthritis were segregated from healthy control subjects based on the circulating T cell subset profile. Among the discriminator subsets, CCR4(+)CXCR3(-) (Th2 and Th17), CTLA4(+) and FOXP3(+) subsets were present in significantly higher frequencies, whereas CCR4(-) (Th1/Th17, CCR6(+)CCR4(-)CXCR3(-), and Th1) subsets were present in lower frequencies in patients with untreated early rheumatoid arthritis compared with healthy control subjects. The proportions of Th2 and Th17 subsets associated positively with each other and negatively with the CXCR3(+)/interferon γ-secreting subsets (Th1 and Th1/Th17) in patients with untreated rheumatoid arthritis. The proportions of Th2 cells increased with age in patients with untreated early rheumatoid arthritis and healthy control subjects. The dominance of circulating CCR4(+)CXCR3(-) T helper subsets (Th2 and Th17) in untreated early rheumatoid arthritis point toward a pathogenic role of

  9. Quasi-geostrophic modes in the Earth's fluid core with an outer stably stratified layer

    Vidal, Jérémie


    Seismic waves sensitive to the outermost part of the Earth's liquid core seem to be affected by a stably stratified layer at the core-mantle boundary. Such a layer could have an observable signature in both long-term and short-term variations of the magnetic field of the Earth, which are used to probe the flow at the top of the core. Indeed, with the recent SWARM mission, it seems reasonable to be able to identify waves propagating in the core with period of several months, which may play an important role in the large-scale dynamics. In this paper, we characterize the influence of a stratified layer at the top of the core on deep quasi-geostrophic (Rossby) waves. We compute numerically the quasi-geostrophic eigenmodes of a rapidly rotating spherical shell, with a stably stratified layer near the outer boundary. Two simple models of stratification are taken into account, which are scaled with commonly accepted values of the Brunt-V{\\"a}is{\\"a}l{\\"a} frequency in the Earth's core. In the absence of magnetic fi...

  10. Spatial characterization of vortical structures and internal waves in stratified turbulent wake using POD

    Diamessis, P.; Gurka, R.; Liberzon, A.


    Proper orthogonal decomposition (POD) is applied to 2-D slices of vorticity and horizontal divergence obtained from the 3-D DNS of the stratified turbulent wake of a towed sphere at Re=5x10^3 and Fr=4. Slices are sampled along the stream-depth (Oxz) and stream-span planes (Oxy) at 231 times during the interval Nt[12,35]. POD was chosen amongst the available statistical tools due to its advantage in characterization of simulated and experimentally measured velocity gradient fields, as previously demonstrated for turbulent boundary layers. In the Oxz planes, at the wake centerline, the higher most energetic modes reveal a structure similar of the structure of late-time stratified wakes. Off-set from centerline, the signature of internal waves in the form of forward-inclined coherent beams extending into the ambient becomes evident. The angle of inclination becomes progressively vertical with increasing POD mode. Lower POD modes on the Oyz planes show a layered structure in the wake core with coherent beams radiating out into the ambient over a broad range of angles. Further insight is provided through the relative energy spectra distribution of the vorticity eigenmodes. POD analysis has provided a statistical description of the geometrical features previously observed in instantaneous flow fields of stratified turbulent wakes.

  11. Node Redeployment Algorithm Based on Stratified Connected Tree for Underwater Sensor Networks

    Jun Liu


    Full Text Available During the underwater sensor networks (UWSNs operation, node drift with water environment causes network topology changes. Periodic node location examination and adjustment are needed to maintain good network monitoring quality as long as possible. In this paper, a node redeployment algorithm based on stratified connected tree for UWSNs is proposed. At every network adjustment moment, self-examination and adjustment on node locations are performed firstly. If a node is outside the monitored space, it returns to the last location recorded in its memory along straight line. Later, the network topology is stratified into a connected tree that takes the sink node as the root node by broadcasting ready information level by level, which can improve the network connectivity rate. Finally, with synthetically considering network coverage and connectivity rates, and node movement distance, the sink node performs centralized optimization on locations of leaf nodes in the stratified connected tree. Simulation results show that the proposed redeployment algorithm can not only keep the number of nodes in the monitored space as much as possible and maintain good network coverage and connectivity rates during network operation, but also reduce node movement distance during node redeployment and prolong the network lifetime.

  12. On a new non-Boussinesq instability in stratified lakes and oceans

    Shete, Mihir H


    Lakes and many other geophysical flows are shallow, density stratified, and contain a free-surface. Conventional studies on stratified shear instabilities make Boussinesq approximation. Free-surface arising due to large density variations between air and water cannot be taken into consideration under this approximation. Hence the free-surface is usually replaced by a rigid-lid, and therefore has little effect on the stability of the fluid below it. In this paper we have performed non-Boussinesq linear stability analyses of a double circulation velocity profile prevalent in two-layered density stratified lakes. One of our analyses is performed by considering the presence of wind, while the other one considers quiescent air. Both analyses have shown similar growth rates and stability boundaries. We have compared our non-Boussinesq study with a corresponding Boussinesq one. The maximum non-Boussinesq growth rate is found to be an order of magnitude greater than the maximum Boussinesq growth rate. Furthermore, th...

  13. Turbulent fluxes of entropy and internal energy in temperature stratified flows

    Rogachevskii, Igor


    We derive equations for the mean entropy and the mean internal energy in the low-Mach-number temperature stratified turbulence (i.e., for turbulent convection or stably stratified turbulence), and show that turbulent flux of entropy is given by ${\\bf F}_s=\\overline{\\rho} \\, \\overline{{\\bf u} s}$, where $\\overline{\\rho}$ is the mean fluid density, $s$ are fluctuations of entropy and overbars denote averaging over an ensemble of turbulent velocity field, ${\\bf u}$. We demonstrate that the turbulent flux of entropy is different from the turbulent convective flux, ${\\bf F}_c=\\overline{T} \\, \\overline{\\rho} \\, \\overline{{\\bf u} s}$, of the fluid internal energy, where $\\overline{T}$ is the mean fluid temperature. This turbulent convective flux is well-known in the astrophysical and geophysical literature, and it cannot be used as a turbulent flux in the equation for the mean entropy. This result is exact for low-Mach-number temperature stratified turbulence and is independent of the model used. We also derive equa...

  14. Waves and vortices in the inverse cascade regime of stratified turbulence with or without rotation

    Herbert, Corentin; Rosenberg, Duane; Pouquet, Annick


    We study the partition of energy between waves and vortices in stratified turbulence, with or without rotation, for a variety of parameters, focusing on the behavior of the waves and vortices in the inverse cascade of energy towards the large scales. To this end, we use direct numerical simulations in a cubic box at a Reynolds number Re=1000, with the ratio between the Brunt-V\\"ais\\"al\\"a frequency N and the inertial frequency f varying from 1/4 to 20, together with a purely stratified run. The Froude number, measuring the strength of the stratification, varies within the range 0.02 < Fr < 0.32. We find that the inverse cascade is dominated by the slow quasi-geostrophic modes. Their energy spectra and fluxes exhibit characteristics of an inverse cascade, even though their energy is not conserved. Surprisingly, the slow vortices still dominate when the ratio N/f increases, also in the stratified case, although less and less so. However, when N/f increases, the inverse cascade of the slow modes becomes we...

  15. Establishment of a novel in vitro model of stratified epithelial wound healing with barrier function.

    Gonzalez-Andrades, Miguel; Alonso-Pastor, Luis; Mauris, Jérôme; Cruzat, Andrea; Dohlman, Claes H; Argüeso, Pablo


    The repair of wounds through collective movement of epithelial cells is a fundamental process in multicellular organisms. In stratified epithelia such as the cornea and skin, healing occurs in three steps that include a latent, migratory, and reconstruction phases. Several simple and inexpensive assays have been developed to study the biology of cell migration in vitro. However, these assays are mostly based on monolayer systems that fail to reproduce the differentiation processes associated to multilayered systems. Here, we describe a straightforward in vitro wound assay to evaluate the healing and restoration of barrier function in stratified human corneal epithelial cells. In this assay, circular punch injuries lead to the collective migration of the epithelium as coherent sheets. The closure of the wound was associated with the restoration of the transcellular barrier and the re-establishment of apical intercellular junctions. Altogether, this new model of wound healing provides an important research tool to study the mechanisms leading to barrier function in stratified epithelia and may facilitate the development of future therapeutic applications.

  16. Turbulent circulation above the surface heat source in stably stratified atmosphere

    Kurbatskii, A. F.; Kurbatskaya, L. I.


    The 3-level RANS approach for simulating a turbulent circulation over the heat island in a stably stratified environment under nearly calm conditions is formulated. The turbulent kinetic energy its spectral consumption (dissipation) and the dispersion of turbulent fluctuations of temperature are found from differential equations, thus the correct modeling of transport processes in the interface layer with the counter-gradient heat flux is assured. The three-parameter turbulence RANS approach minimizes difficulties in simulating the turbulent transport in a stably stratified environment and reduces efforts needed for the numerical implementation of the 3-level RANS approach. Numerical simulation of the turbulent structure of the penetrative convection over the heat island under conditions of stably stratified atmosphere demonstrates that the three-equation model is able to predict the thermal circulation induced by the heat island. The temperature distribution, root-mean-square fluctuations of the turbulent velocity and temperature fields and spectral turbulent kinetic energy flux are in good agreement with the experimental data. The model describes such thin physical effects, as a crossing of vertical profiles of temperature of a thermal plume with the formation of the negative buoyancy area testifying to development of the dome-shaped form at the top part of a plume in the form of "hat".

  17. An affordable and accurate conductivity probe for density measurements in stratified flows

    Carminati, Marco; Luzzatto-Fegiz, Paolo


    In stratified flow experiments, conductivity (combined with temperature) is often used to measure density. The probes typically used can provide very fine spatial scales, but can be fragile, expensive to replace, and sensitive to environmental noise. A complementary instrument, comprising a low-cost conductivity probe, would prove valuable in a wide range of applications where resolving extremely small spatial scales is not needed. We propose using micro-USB cables as the actual conductivity sensors. By removing the metallic shield from a micro-B connector, 5 gold-plated microelectrodes are exposed and available for 4-wire measurements. These have a cell constant ~550m-1, an intrinsic thermal noise of at most 30pA/Hz1/2, as well as sub-millisecond time response, making them highly suitable for many stratified flow measurements. In addition, we present the design of a custom electronic board (Arduino-based and Matlab-controlled) for simultaneous acquisition from 4 sensors, with resolution (in conductivity, and resulting density) exceeding the performance of typical existing probes. We illustrate the use of our conductivity-measuring system through stratified flow experiments, and describe plans to release simple instructions to construct our complete system for around 200.

  18. Stress-Induced In Vivo Recruitment of Human Cytotoxic Natural Killer Cells Favors Subsets with Distinct Receptor Profiles and Associates with Increased Epinephrine Levels.

    Marc B Bigler

    Full Text Available Acute stress drives a 'high-alert' response in the immune system. Psychoactive drugs induce distinct stress hormone profiles, offering a sought-after opportunity to dissect the in vivo immunological effects of acute stress in humans.3,4-methylenedioxymethamphetamine (MDMA, methylphenidate (MPH, or both, were administered to healthy volunteers in a randomized, double-blind, placebo-controlled crossover-study. Lymphocyte subset frequencies, natural killer (NK cell immune-phenotypes, and changes in effector function were assessed, and linked to stress hormone levels and expression of CD62L, CX3CR1, CD18, and stress hormone receptors on NK cells.MDMA/MPH > MDMA > MPH robustly induced an epinephrine-dominant stress response. Immunologically, rapid redistribution of peripheral blood lymphocyte-subsets towards phenotypically mature NK cells occurred. NK cytotoxicity was unaltered, but they expressed slightly reduced levels of the activating receptor NKG2D. Preferential circulation of mature NK cells was associated with high epinephrine receptor expression among this subset, as well as expression of integrin ligands previously linked to epinephrine-induced endothelial detachment.The acute epinephrine-induced stress response was characterized by rapid accumulation of mature and functional NK cells in the peripheral circulation. This is in line with studies using other acute stressors and supports the role of the acute stress response in rapidly mobilizing the innate immune system to counteract incoming threats.

  19. Fractional randomness

    Tapiero, Charles S.; Vallois, Pierre


    The premise of this paper is that a fractional probability distribution is based on fractional operators and the fractional (Hurst) index used that alters the classical setting of random variables. For example, a random variable defined by its density function might not have a fractional density function defined in its conventional sense. Practically, it implies that a distribution's granularity defined by a fractional kernel may have properties that differ due to the fractional index used and the fractional calculus applied to define it. The purpose of this paper is to consider an application of fractional calculus to define the fractional density function of a random variable. In addition, we provide and prove a number of results, defining the functional forms of these distributions as well as their existence. In particular, we define fractional probability distributions for increasing and decreasing functions that are right continuous. Examples are used to motivate the usefulness of a statistical approach to fractional calculus and its application to economic and financial problems. In conclusion, this paper is a preliminary attempt to construct statistical fractional models. Due to the breadth and the extent of such problems, this paper may be considered as an initial attempt to do so.

  20. Spatial-temporal subset based digital image correlation considering the temporal continuity of deformation

    Wang, Xian; Liu, Xuejin; Zhu, Haibin; Ma, Shaopeng


    An improved digital image correlation (DIC) scheme termed spatial-temporal subset-based DIC (STS-DIC) that incorporates the temporal continuity of deformation is proposed. Provided that displacement at a certain physical point on a specimen in several successive frames is temporally continuous and can be expressed as a linear relationship over time, the STS-DIC scheme is constructed between the reference subset and spatial-temporal deformed subset consisting of several subsets from a period of successive frames. The proposed method is verified by simulated speckle images and experimental tests featuring different types of deformation. Compared to the traditional subset-based DIC, the STS-DIC proposed in this paper takes advantage of noise suppression so as to improve the accuracy, especially for speckle images with larger noise. More importantly, it is found that the computational demand of STS-DIC is much lower than that of mesh-based (global) DIC incorporating the temporal continuity, despite achieving comparable accuracy. Therefore, STS-DIC is expected to be useful as a practical and flexible tool in complex-environment measurements with low signal-to-noise-ratio speckle images.

  1. Increased Peripheral Proinflammatory T Helper Subsets Contribute to Cardiovascular Complications in Diabetic Patients

    Ru-xing Zhao


    Full Text Available Background. Coronary atherosclerotic heart disease (CHD is one of the major concerns in type 2 diabetes (T2D. The systemic chronic inflammation has been postulated to bridge the increased risk of cardiovascular disease and T2D. We formulated that increased peripheral proinflammatory T helper subsets contributed to the development of cardiovascular complications in diabetic patients. Methods. The frequencies of peripheral total CD4+ T helper cells, proinflammatory Th1, Th17, and Th22 subsets were determined by flow cytometry in diabetic patients with or without CHD (n=42 and 67, resp.. Results. Both peripheral frequencies and total numbers of Th1, Th17, and Th22 cells were further increased in diabetic patients with CHD. Logistic regression and categorical cross-table analysis further confirmed that increased proinflammatory Th subsets, especially Th22, were independent risk factors of cardiovascular complication in diabetes. Elevated Th subsets also correlated with increased CRP levels and the atherogenic index of plasma. Moreover, Th1 frequency and Th22 numbers demonstrated remarkable potential in predicting CHD in diabetes. Conclusions. Increased peripheral proinflammatory T helper subsets act in concert and contribute to the increased prevalence of diabetic cardiovasculopathy. The recently identified Th22 cells might play an independent role in CHD and represent a novel proxy for cardiovascular risks in diabetes.

  2. Label-free haemogram using wavelength modulated Raman spectroscopy for identifying immune-cell subset

    Ashok, Praveen C.; Praveen, Bavishna B.; Campbell, Elaine C.; Dholakia, Kishan; Powis, Simon J.


    Leucocytes in the blood of mammals form a powerful protective system against a wide range of dangerous pathogens. There are several types of immune cells that has specific role in the whole immune system. The number and type of immune cells alter in the disease state and identifying the type of immune cell provides information about a person's state of health. There are several immune cell subsets that are essentially morphologically identical and require external labeling to enable discrimination. Here we demonstrate the feasibility of using Wavelength Modulated Raman Spectroscopy (WMRS) with suitable machine learning algorithms as a label-free method to distinguish between different closely lying immune cell subset. Principal Component Analysis (PCA) was performed on WMRS data from single cells, obtained using confocal Raman microscopy for feature reduction, followed by Support Vector Machine (SVM) for binary discrimination of various cell subset, which yielded an accuracy >85%. The method was successful in discriminating between untouched and unfixed purified populations of CD4+CD3+ and CD8+CD3+ T lymphocyte subsets, and CD56+CD3- natural killer cells with a high degree of specificity. It was also proved sensitive enough to identify unique Raman signatures that allow clear discrimination between dendritic cell subsets, comprising CD303+CD45+ plasmacytoid and CD1c+CD141+ myeloid dendritic cells. The results of this study clearly show that WMRS is highly sensitive and can distinguish between cell types that are morphologically identical.

  3. A dengue vector surveillance by human population-stratified ovitrap survey for Aedes (Diptera: Culicidae) adult and egg collections in high dengue-risk areas of Taiwan.

    Wu, Huai-Hui; Wang, Chih-Yuan; Teng, Hwa-Jen; Lin, Cheo; Lu, Liang-Chen; Jian, Shu-Wan; Chang, Niann-Tai; Wen, Tzai-Hung; Wu, Jhy-Wen; Liu, Ding-Ping; Lin, Li-Jen; Norris, Douglas E; Wu, Ho-Sheng


    Aedes aegypti L. is the primary dengue vector in southern Taiwan. This article is the first report on a large-scale surveillance program to study the spatial-temporal distribution of the local Ae. aegytpi population using ovitraps stratified according to the human population in high dengue-risk areas. The sampling program was conducted for 1 yr and was based on weekly collections of eggs and adults in Kaohsiung City. In total, 10,380 ovitraps were placed in 5,190 households. Paired ovitraps, one indoors and one outdoors were used per 400 people. Three treatments in these ovitraps (paddle-shaped wooden sticks, sticky plastic, or both) were assigned by stratified random sampling to two areas (i.e., metropolitan or rural, respectively). We found that the sticky plastic alone had a higher sensitivity for detecting the occurrence of indigenous dengue cases than other treatments with time lags of up to 14 wk. The wooden paddle alone detected the oviposition of Ae. aegypti throughout the year in this study area. Furthermore, significantly more Ae. aegypti females were collected indoors than outdoors. Therefore, our survey identified the whole year oviposition activity, spatial-temporal distribution of the local Ae. aegypti population and a 14 wk lag correlation with dengue incidence to plan an effectively proactive control.

  4. On Maximal Ranges of Vector Measures for Subsets and Purification of Transition Probabilities

    Dai, Peng


    Consider a measurable space with an atomless finite vector measure. This measure defines a mapping of the $\\sigma$-field into an Euclidean space. According to the Lyapunov convexity theorem, the range of this mapping is a convex compactum. Similar ranges are also defined for measurable subsets of the space. Two subsets with the same vector measure may have different ranges. We investigate the question whether, among all the subsets having the same given vector measure, there always exists a set with the maximal range of the vector measure. The answer to this question is positive for two-dimensional vector measures and negative for higher dimensions. We use this fact to prove that for two-dimensional vector measures the Dvoretzky-Wald-Wolfowitz purification theorem holds for the case of a countable image set.

  5. Digital image correlation involves an inverse problem: A regularization scheme based on subset size constraint

    Zhan, Qin; Yuan, Yuan; Fan, Xiangtao; Huang, Jianyong; Xiong, Chunyang; Yuan, Fan


    Digital image correlation (DIC) is essentially implicated in a class of inverse problem. Here, a regularization scheme is developed for the subset-based DIC technique to effectively inhibit potential ill-posedness that likely arises in actual deformation calculations and hence enhance numerical stability, accuracy and precision of correlation measurement. With the aid of a parameterized two-dimensional Butterworth window, a regularized subpixel registration strategy is established, in which the amount of speckle information introduced to correlation calculations may be weighted through equivalent subset size constraint. The optimal regularization parameter associated with each individual sampling point is determined in a self-adaptive way by numerically investigating the curve of 2-norm condition number of coefficient matrix versus the corresponding equivalent subset size, based on which the regularized solution can eventually be obtained. Numerical results deriving from both synthetic speckle images and actual experimental images demonstrate the feasibility and effectiveness of the set of newly-proposed regularized DIC algorithms.

  6. Crossing the Vascular Wall: Common and Unique Mechanisms Exploited by Different Leukocyte Subsets during Extravasation

    Michael Schnoor


    Full Text Available Leukocyte extravasation is one of the essential and first steps during the initiation of inflammation. Therefore, a better understanding of the key molecules that regulate this process may help to develop novel therapeutics for treatment of inflammation-based diseases such as atherosclerosis or rheumatoid arthritis. The endothelial adhesion molecules ICAM-1 and VCAM-1 are known as the central mediators of leukocyte adhesion to and transmigration across the endothelium. Engagement of these molecules by their leukocyte integrin receptors initiates the activation of several signaling pathways within both leukocytes and endothelium. Several of such events have been described to occur during transendothelial migration of all leukocyte subsets, whereas other mechanisms are known only for a single leukocyte subset. Here, we summarize current knowledge on regulatory mechanisms of leukocyte extravasation from a leukocyte and endothelial point of view, respectively. Specifically, we will focus on highlighting common and unique mechanisms that specific leukocyte subsets exploit to succeed in crossing endothelial monolayers.

  7. Changes in percentage of lymphocyte subsets after 131I treatment in patients with differentiated thyroid cancer

    LUO Quan-Yong; CHEN Li-Bo; YU Yong-Li; LU Han-Kui; ZHU Rui-Sen


    To monitor the extent and the duration of lymphocyte subset changes in patients with thyroid carcinoma undergoing therapeutic 131I administration, the percentage of lymphocyte subsets were serially analyzed before and after 131I treatment. In patients who received 1850 MBq of 131I for ablation of thyroid remnants, only for NK cells and B cells showed a significant reduction. In patients received 3700 MBq of 131I for treatment of local lymph node metastases, NK cells, B cells and CD4+ were found decreased. In patients received 7400 MBq of 131I for treatment of distant metastases, NK cells, B cells and CD4+ and CD8+ were all affected. However, there is no significant reduction compared to the baseline in the percentage of all lymphocyte subsets three months after 131I treatment. The results show that the sensitivity of lymphocytes to 131I internal radiation depends upon lymphocyte phenotype and 131I activity. The immunosuppression effects are temporary and reversible.

  8. Leukocyte Subset Changes in Response to a 164-km Road Cycle Ride in a Hot Environment



    The purpose of this observational study was to determine the circulating leukocyte subset response to completing the 2013 Hotter’N Hell Hundred recreational 164-km road cycle event in a hot and humid environmental condition. Twenty-eight men and four women were included in this study. Whole blood samples were obtained 1–2 hours before (PRE) and immediately after (POST) the event. Electronic sizing/sorting and cytometry were used to determine complete blood counts (CBC) including neutrophil, monocyte, and lymphocyte subsets. The concentration of circulating total leukocytes (103·μL−1) increased 134% from PRE to POST with the greatest increase in neutrophils (319%, pexercise in stressful environmental conditions affects the complement of circulating immune cells, although activational state and characterization of specific leukocyte subsets remains unclear. The observed increase in circulating cell sub-populations suggests that the circulating immune surveillance system may be acutely affected by exercise in hot and humid conditions. PMID:27293505

  9. Implications of Air Ingress Induced by Density-Difference Driven Stratified Flow

    Chang Oh; Eung Soo Kim; Richard Schultz; David Petti; C. P. Liou


    One of the design basis accidents for the Next Generation Nuclear Plant (NGNP), a high temperature gas-cooled reactor, is air ingress subsequent to a pipe break. Following a postulated double-ended guillotine break in the hot duct, and the subsequent depressurization to nearly reactor cavity pressure levels, air present in the reactor cavity will enter the reactor vessel via density-gradient-driven-stratified flow. Because of the significantly higher molecular weight and lower initial temperature of the reactor cavity air-helium mixture, in contrast to the helium in the reactor vessel, the air-helium mixture in the cavity always has a larger density than the helium discharging from the reactor vessel through the break into the reactor cavity. In the later stages of the helium blowdown, the momentum of the helium flow decreases sufficiently for the heavier cavity air-helium mixture to intrude into the reactor vessel lower plenum through the lower portion of the break. Once it has entered, the heavier gas will pool at the bottom of the lower plenum. From there it will move upwards into the core via diffusion and density-gradient effects that stem from heating the air-helium mixture and from the pressure differences between the reactor cavity and the reactor vessel. This scenario (considering density-gradient-driven stratified flow) is considerably different from the heretofore commonly used scenario that attributes movement of air into the reactor vessel and from thence to the core region via diffusion. When density-gradient-driven stratified flow is considered as a contributing phenomena for air ingress into the reactor vessel, the following factors contribute to a much earlier natural circulation-phase in the reactor vessel: (a) density-gradient-driven stratified flow is a much more rapid mechanism (at least one order of magnitude) for moving air into the reactor vessel lower plenum than diffusion, and consequently, (b) the diffusion dominated phase begins with a

  10. A systems biology approach to the analysis of subset-specific responses to lipopolysaccharide in dendritic cells.

    David G Hancock

    Full Text Available Dendritic cells (DCs are critical for regulating CD4 and CD8 T cell immunity, controlling Th1, Th2, and Th17 commitment, generating inducible Tregs, and mediating tolerance. It is believed that distinct DC subsets have evolved to control these different immune outcomes. However, how DC subsets mount different responses to inflammatory and/or tolerogenic signals in order to accomplish their divergent functions remains unclear. Lipopolysaccharide (LPS provides an excellent model for investigating responses in closely related splenic DC subsets, as all subsets express the LPS receptor TLR4 and respond to LPS in vitro. However, previous studies of the LPS-induced DC transcriptome have been performed only on mixed DC populations. Moreover, comparisons of the in vivo response of two closely related DC subsets to LPS stimulation have not been reported in the literature to date. We compared the transcriptomes of murine splenic CD8 and CD11b DC subsets after in vivo LPS stimulation, using RNA-Seq and systems biology approaches. We identified subset-specific gene signatures, which included multiple functional immune mediators unique to each subset. To explain the observed subset-specific differences, we used a network analysis approach. While both DC subsets used a conserved set of transcription factors and major signalling pathways, the subsets showed differential regulation of sets of genes that 'fine-tune' the network Hubs expressed in common. We propose a model in which signalling through common pathway components is 'fine-tuned' by transcriptional control of subset-specific modulators, thus allowing for distinct functional outcomes in closely related DC subsets. We extend this analysis to comparable datasets from the literature and confirm that our model can account for cell subset-specific responses to LPS stimulation in multiple subpopulations in mouse and man.

  11. Hierarchical modeling for rare event detection and cell subset alignment across flow cytometry samples.

    Andrew Cron

    Full Text Available Flow cytometry is the prototypical assay for multi-parameter single cell analysis, and is essential in vaccine and biomarker research for the enumeration of antigen-specific lymphocytes that are often found in extremely low frequencies (0.1% or less. Standard analysis of flow cytometry data relies on visual identification of cell subsets by experts, a process that is subjective and often difficult to reproduce. An alternative and more objective approach is the use of statistical models to identify cell subsets of interest in an automated fashion. Two specific challenges for automated analysis are to detect extremely low frequency event subsets without biasing the estimate by pre-processing enrichment, and the ability to align cell subsets across multiple data samples for comparative analysis. In this manuscript, we develop hierarchical modeling extensions to the Dirichlet Process Gaussian Mixture Model (DPGMM approach we have previously described for cell subset identification, and show that the hierarchical DPGMM (HDPGMM naturally generates an aligned data model that captures both commonalities and variations across multiple samples. HDPGMM also increases the sensitivity to extremely low frequency events by sharing information across multiple samples analyzed simultaneously. We validate the accuracy and reproducibility of HDPGMM estimates of antigen-specific T cells on clinically relevant reference peripheral blood mononuclear cell (PBMC samples with known frequencies of antigen-specific T cells. These cell samples take advantage of retrovirally TCR-transduced T cells spiked into autologous PBMC samples to give a defined number of antigen-specific T cells detectable by HLA-peptide multimer binding. We provide open source software that can take advantage of both multiple processors and GPU-acceleration to perform the numerically-demanding computations. We show that hierarchical modeling is a useful probabilistic approach that can provide a

  12. Data mining a small molecule drug screening representative subset from NIH PubChem.

    Xie, Xiang-Qun; Chen, Jian-Zhong


    PubChem is a scientific showcase of the NIH Roadmap Initiatives. It is a compound repository created to facilitate information exchange and data sharing among the NIH Roadmap-funded Molecular Library Screening Center Network (MLSCN) and the scientific community. However, PubChem has more than 10 million records of compound information. It will be challenging to conduct a drug screening of the whole database of millions of compounds. Thus, the purpose of the present study was to develop a data mining cheminformatics approach in order to construct a representative and structure-diverse sublibrary from the large PubChem database. In this study, a new chemical diverse representative subset, rePubChem, was selected by whole-molecule chemistry-space matrix calculation using the cell-based partition algorithm. The representative subset was generated and was then subjected to evaluations by compound property analyses based on 1D and 2D molecular descriptors. The new subset was also examined and assessed for self-similarity analysis based on 2D molecular fingerprints in comparing with the source compound library. The new subset has a much smaller library size (540K compounds) with minimum similarity and redundancy without loss of the structural diversity and basic molecular properties of its parent library (5.3 million compounds). The new representative subset library generated could be a valuable structure-diverse compound resource for in silico virtual screening and in vitro HTS drug screening. In addition, the established subset generation method of using the combined cell-based chemistry-space partition metrics with pairwised 2D fingerprint-based similarity search approaches will also be important to a broad scientific community interested in acquiring structurally diverse compounds for efficient drug screening, building representative virtual combinatorial chemistry libraries for syntheses, and data mining large compound databases like the PubChem library in general.

  13. Longterm decrease in the CD57 lymphocyte subset in a patient with chronic Lyme disease.

    Stricker, Raphael B; Burrascano, Joseph; Winger, Edward


    Lyme disease is a tickborne illness caused by the spirochete Borrelia burgdorferi. In a previous report we described a decrease in the CD57 lymphocyte subset in patients with chronic Lyme disease. We have now identified a patient with chronic relapsing and remitting symptoms of Lyme disease who had decreased levels of CD57 lymphocytes over 10 years. This observation represents the longest duration of an immunologic abnormality ever documented in chronic Lyme disease. The CD57 lymphocyte subset appears to be a useful marker of longterm infection with the Lyme disease spirochete.

  14. Gamma-Ray Bursts Subset and Supernova Remnants Low Radio-Frequency Turnover

    LIU Xiang


    Durations of gamma-ray bursts (GRB's) are featured by >2s subset and <2s one, with initial corresponding energy ratio being 20:1. It is found that supernova remants(SNR 's) turnover frequencies peak at 100 and 500 MHz. After assuming that GRB's originate from hypernova and making an analysis on the evolution of GRB's, we find that the initial energy of two GRB subsets leads to a different radio-frequency turnover of their remnant spectra, which accords positively with the turnover-frequency ratio of SNR's.

  15. Technical Note: Proximal Ordered Subsets Algorithms for TV Constrained Optimization in CT Image Reconstruction

    Rose, Sean; Sidky, Emil Y; Pan, Xiaochuan


    This article is intended to supplement our 2015 paper in Medical Physics titled "Noise properties of CT images reconstructed by use of constrained total-variation, data-discrepancy minimization", in which ordered subsets methods were employed to perform total-variation constrained data-discrepancy minimization for image reconstruction in X-ray computed tomography. Here we provide details regarding implementation of the ordered subsets algorithms and suggestions for selection of algorithm parameters. Detailed pseudo-code is included for every algorithm implemented in the original manuscript.

  16. Parameter subset selection for the dynamic calibration of activated sludge models (ASMs): experience versus systems analysis

    Ruano, MV; Ribes, J; de Pauw, DJW


    In this work we address the issue of parameter subset selection within the scope of activated sludge model calibration. To this end, we evaluate two approaches: (i) systems analysis and (ii) experience-based approach. The evaluation has been carried out using a dynamic model (ASM2d) calibrated...... based approaches which excluded them from their analysis. Systems analysis reveals that parameter significance ranking and size of the identifiable parameter subset depend on the information content of data available for calibration. However, it suffers from heavy computational demand. In contrast...

  17. How to create order in large closed subsets of WordNet-type dictionaries

    Ahti Lohk


    Full Text Available This article presents a new two-step method to handle and study large closed subsets of WordNet-type dictionaries with the goal of finding possible structural inconsistencies. The notion of closed subset is explained using a WordNet tree. A novel and very fast method to order large relational systems is described and compared with some other fast methods. All the presented methods have been tested using Estonian1 and Princeton WordNet2 largest closed sets.DOI:

  18. 基于Kriging模型的子集模拟方法%Subset Simulation Based on Kriging Model

    闫阳; 董聪; 陈向前


    Study how to estimate failure probability of structure accurately. For actual structures, it is necessary to do structural analysis for each random sample. In the case of complicated structure, the big number of random sample leads huge computational costs, which makes the computational time unacceptable or the model not convergence. The traditional failure probability estimating methods are based on Kriging model, which is influenced by the model stability, usually does not work effectively. In this paper, we proposed a Subset Simulation method Based on Kriging Model, which searches training samples via learning function, establishes Kriging Models for each intermediate failure domain, and computes failure probability by Kriging predictive values instead of actual function values. The numerical examples show that the proposed method needs only a small number of structural analyses to get the similar results as Subset Simulation. Meanwhile, the proposed method can be connected easily with the finite element method, which leads a great reduction of computational costs.%研究建筑结构失效的准确判断问题.在实际工程,需对每个随机样本点进行结构分析,一旦建筑工程较大,需要采集的随机样本数量较大,需要较大的计算成本,造成计算的耗时,甚至模型不收敛.传统的基于Kriging模型的工程结构失效判断方法,受到模型不稳定的影响,影响建筑失效判断的准确性.为此提出了基于Kriging模型的子集模拟方法,引入学习函数主动寻找并添加训练样本,对每个中间失效域建立对应的Kriging模型,用Kriging模型的预测值代替结构实际功能函数值进行仿真计算.实验仿真表明,改进模型只需要进行少量的结构分析就能得到与子集模拟方法类似的仿真结果,可以和有限元程序对接,能够大幅度降低仿真方法的计算代价.

  19. Analysis of flame propagation phenomenon in simplified stratified charge conditions; Tanjunkasareta sojo kyukiba ni okeru kaen denpa gensho no kansatsu

    Moriyoshi, Y.; Morikawa, H. [Chiba University, Chiba (Japan); Kamimoto, T. [Tokyo Institute of Technology, Tokyo (Japan)


    Since the local inhomogeneity of mixture concentration inside the cylinder affects the combustion characteristics, a basic research on combustion phenomenon in stratified charge conditions is required. The authors have made experiments with a constant-volume chamber, which can simulate an idealized stratified charge field by using a removable partition, to obtain the combustion characteristics. Also, numerical calculations are made using some combustion models. As a result, the important feature that the combustion speed is faster in stratified condition than in homogeneous condition can be predicted by the two-step reaction model. 4 refs., 8 figs.

  20. A model of particle removal in a dissolved air flotation tank: importance of stratified flow and bubble size.

    Lakghomi, B; Lawryshyn, Y; Hofmann, R


    An analytical model and a computational fluid dynamic model of particle removal in dissolved air flotation were developed that included the effects of stratified flow and bubble-particle clustering. The models were applied to study the effect of operating conditions and formation of stratified flow on particle removal. Both modeling approaches demonstrated that the presence of stratified flow enhanced particle removal in the tank. A higher air fraction was shown to be needed at higher loading rates to achieve the same removal efficiency. The model predictions showed that an optimum bubble size was present that increased with an increase in particle size.