Environment and the sterile insect technique

International Nuclear Information System (INIS)

Nagel, P.; Peveling, R.

2005-01-01

The sterile insect technique (SIT) is an exceptionally promising pest control method in terms of efficacy and environmental compatibility. Assessments of environmental risks vary according to the status and origin of the target pests. The suppression or eradication of exotic pest populations with the SIT raises few environmental concerns, and these are related mainly to pre-release suppression techniques. However, the elimination of native species, or at least populations of native species, requires more detailed and complex assessments of ecological effects and consequences for biodiversity conservation. Eradication programmes provide opportunities to study these topics within the scope of both environmental impact assessments and operational monitoring programmes. (author)

Sterilizing insects with ionizing radiation

International Nuclear Information System (INIS)

Bakri, A.; Mehta, K.; Lance, D.R.

2005-01-01

Exposure to ionizing radiation is currently the method of choice for rendering insects reproductively sterile for area-wide integrated pest management (AW-IPM) programmes that integrate the sterile insect technique (SIT). Gamma radiation from isotopic sources (cobalt-60 or caesium-137) is most often used, but high-energy electrons and X-rays are other practical options. Insect irradiation is safe and reliable when established safety and quality-assurance guidelines are followed. The key processing parameter is absorbed dose, which must be tightly controlled to ensure that treated insects are sufficiently sterile in their reproductive cells and yet able to compete for mates with wild insects. To that end, accurate dosimetry (measurement of absorbed dose) is critical. Irradiation data generated since the 1950s, covering over 300 arthropod species, indicate that the dose needed for sterilization of arthropods varies from less than 5 Gy for blaberid cockroaches to 300 Gy or more for some arctiid and pyralid moths. Factors such as oxygen level, and insect age and stage during irradiation, and many others, influence both the absorbed dose required for sterilization and the viability of irradiated insects. Consideration of these factors in the design of irradiation protocols can help to find a balance between the sterility and competitiveness of insects produced for programmes that release sterile insects. Many programmes apply 'precautionary' radiation doses to increase the security margin of sterilization, but this overdosing often lowers competitiveness to the point where the overall induced sterility in the wild population is reduced significantly. (author)

History of the sterile insect technique

International Nuclear Information System (INIS)

Klassen, W.; Curtis, C.F.

2005-01-01

During the 1930s and 1940s the idea of releasing insects of pest species to introduce sterility (sterile insect technique or SIT) into wild populations, and thus control them, was independently conceived in three extremely diverse intellectual environments. The key researchers were A. S. Serebrovskii at Moscow State University, F. L. Vanderplank at a tsetse field research station in rural Tanganyika (now Tanzania), and E. F. Knipling of the United States Department of Agriculture. Serebrovskii's work on chromosomal translocations for pest population suppression could not succeed in the catastrophic conditions in the USSR during World War II, after which he died. Vanderplank used hybrid sterility to suppress a tsetse population in a large field experiment, but lacked the resources to develop this method further. Knipling and his team exploited H. J. Muller's discovery that ionizing radiation can induce dominant lethal mutations, and after World War II this approach was applied on an area-wide basis to eradicate the New World screwworm Cochliomyia hominivorax (Coquerel) in the USA, Mexico, and Central America. Since then very effective programmes integrating the SIT have been mounted against tropical fruit flies, some species of tsetse flies Glossina spp., the pink bollworm Pectinophora gossypiella (Saunders), and the codling moth Cydia pomonella (L.). In non-isolated onion fields in the Netherlands, the onion maggot Delia antiqua (Meigen) has since 1981 been suppressed by the SIT. In the 1970s there was much research conducted on mosquito SIT, which then went into 'eclipse', but now appears to be reviving. Development of the SIT for use against the boll weevil Anthonomus grandis grandis Boheman and the gypsy moth Lymantria dispar (L.) has ended, but it is in progress for two sweetpotato weevil species, Cylas formicarius (F.) and Euscepes postfasciatus (Fairmaire), the false codling moth Cryptophlebia leucotreta (Meyrick), the carob moth Ectomyelois ceratoniae

First results in the use of sterile insect technique against Trialeurodes vaporariorum (Homoptera: Aleyroididae) in greenhouses

International Nuclear Information System (INIS)

Calvitti, M.; Remotti, P.C.; Pasquali, A.; Cirio, U.

1998-01-01

Trials for the evaluation of the effectiveness of the sterile insect technique for the suppression of greenhouse whitefly, Trialeurodes vaporariorum (Westwood), both in cage and in greenhouse conditions are described. The results show a significant reduction of the reproductive capacity of the untreated whitefly populations interacting with sterile insects. Untreated whiteflies, co-existing in a mixed population together with sterile insects, attained less than the half (44%) of their potential reproductive capacity. This trend was also evident in the cage test where the untreated whitefly population, crossed with the sterile whiteflies, increased without exceeding 2/3 of the density recorded in the control cages. These results may be based on 2 joint sterile insect technique effects: primarily a drastic reduction of the progeny of normal untreated females, when mating with sterile males, carriers of dominant lethal mutations, and secondarily a progressive reduction of the females in the population due to an increasing rate of unsuccessful matings resulting in a condition of forced arrhenotoky. No deleterious effects, on plant health and fruit quality, were observed on plants exposed to high sterile whitefly pressures

Population suppression in support of the sterile insect technique

International Nuclear Information System (INIS)

Mangan, R.L.

2005-01-01

Suppression or eradication of insect pest populations by the release of sterile insects is often dependent on supplementary methods of pest reduction to levels where the target pest population can be overflooded with sterile insects. Population suppression activities take place in advance of, or coincide with, the production of sterile insects. Supplementary methods to remove breeding opportunities, or management methods that prevent access of pests to the hosts, may reduce the population or prevent damage. Insecticides have been used widely in direct applications or applied as baits, in traps, or on specific sites where the pest makes contact or reproduces. As sterile insect release does not kill the pest, adult biting pests or fertile mated females of the pests will continue to attack hosts after the release of sterile insects. Thus supplementary pest suppression programmes and quarantine measures are essential to prevent damage or the spread of disease. Eradication or effective pest management requires that the entire population of the pest be treated, or that the programme apply immigration barriers. When supplementary pest control activities benefit the human population in areas being treated, such as in mosquito or screwworm eradication programmes, these activities are usually acceptable to the public, but when the public receives no direct benefit from supplementary control activities such as in fruit fly programmes, social resistance may develop. (author)

Sterile insect supply, emergence, and release

International Nuclear Information System (INIS)

Dowell, R.V.; Worley, J.; Gomes, P.J.

2005-01-01

Insect mass-rearing for a sterile insect technique (SIT) programme is designed to move beyond the large-scale rearing of insects in a laboratory to the industrial production of consistently high-quality insects for sterilization and release. Each facility reflects the unique biology of the insect reared within it, but there are some generalities for all rearing facilities. Rearing insects in self-contained modules offers flexibility, and increased safety from catastrophic occurrences, compared with using a single building which houses all facets of the rearing process. Although mechanizing certain aspects of the rearing steps helps provide a consistently high-quality insect, successful mass-rearing and delivery depends largely upon the human component. Besides production in centralized facilities, insects can be produced from purchased eggs, or nowadays, adult insects are often obtained from specialized satellite emergence/collection facilities. Interest in commercializing insect production and release is increasing. Shipping sterile insects, sometimes over long distances, is now common practice. Procedures for handling and chilling adult insects, and providing food and water prior to release, are continually being improved. Sterile insects are released via static-release receptacles, ground-release systems, or most commonly from the air. The aerial release of chilled sterile insects is the most efficient method of release, especially when aircraft flight paths are guided by a Global Positioning System (GPS) linked to a computer-controlled release mechanism. (author)

Use of sterile insect technique in Brazil by the sterilization of Ceratitis capitata (Wied., 1824)

International Nuclear Information System (INIS)

Okeese, G.O.; Kitomer, T.J.; Gayaradus, L.B.; Etrigan, M.; Jansen, C.W.J.H.; Houlahar, H.L.

2006-01-01

The Sterile Insect Technique is a method of pest control allowed in Integrated Pest Management programs in fruit growing, where sterile insects released compete in mate process against fertile wild ones, generating a gradative population reduction. The most used sterilization method is using ionizing radiation from 60 Co or 137 Cs. For efficient application of SIT, an important item is the sterilizing dose. This must be as lower as possible in order to preserve insect behavior. In this work, it was studied the reproductive potential of fruit fly Ceratitis capitata, tsl VIENNA 8 strain, irradiated with different doses of gamma radiation. This strain has been used in population suppression programs at fruit-growing areas from Northeast of Brazil, through a partnership between CENA and Bio factory MOSCAMED Brazil, intending only sterile male releases. Radiation source used was a 60 Co irradiator, with 17.2 x 1012 Bq. Individualized couples were distributed in a randomized delineament with 10 replicates for each treatment and eggs were collected for fifteen days. With 9 and 10 days old, pupae were irradiated with doses ranging from 30 to 120 Gy. All averages of emerged adult's percentages were higher than 90%. In regards to fecundity, different doses did not affect the number of eggs laid, being the overall mean around 30 eggs/couple/day. Fertility data showed that as radiation dose increases, sterility increases. It was concluded that the dose of 90 Gy can be used in Brazil, since to SIT programs a dose is chosen witch prevents an egg hatch higher than 1%. (author)

Mass-rearing for sterile insect release

International Nuclear Information System (INIS)

Parker, A.G.

2005-01-01

As the sterile insect technique (SIT) relies upon released sterile male insects efficiently competing with wild males to mate with wild females, it follows that mass-rearing of insects is one of the principal steps in the process. Mass-rearing for the SIT presents both problems and opportunities due to the increased scale involved compared with rearing insects for most other purposes. This chapter discusses facility design, environmental concerns, strain management, quality control, automation, diet, sex separation, marking, and storage in relation to rearing for the SIT. (author)

The sterile-insect technique for the control of fruit flies: a survey

International Nuclear Information System (INIS)

Harris, E.J.

1975-01-01

Some advantages of the sterile-insect technique (SIT) are its minimum contribution to environmental pollution and its minimum adverse effect on non-target organisms. A review is made of the melon fly and sterile Mediterranean fruit fly release programmes, the accomplishments, and the implications. Recommendations are made for research leading to development of methods for practical use of the SIT. (author)

Modelling the effects of the sterile insect technique applied to Eldana saccharina Walker in sugarcane

Directory of Open Access Journals (Sweden)

L Potgieter

2012-12-01

Full Text Available A mathematical model is formulated for the population dynamics of an Eldana saccharina Walker infestation of sugarcane under the influence of partially sterile released insects. The model describes the population growth of and interaction between normal and sterile E.saccharina moths in a temporally variable, but spatially homogeneous environment. The model consists of a deterministic system of difference equations subject to strictly positive initial data. The primary objective of this model is to determine suitable parameters in terms of which the above population growth and interaction may be quantified and according to which E.saccharina infestation levels and the associated sugarcane damage may be measured. Although many models have been formulated in the past describing the sterile insect technique, few of these models describe the technique for Lepidopteran species with more than one life stage and where F1-sterility is relevant. In addition, none of these models consider the technique when fully sterile females and partially sterile males are being released. The model formulated is also the first to describe the technique applied specifically to E.saccharina, and to consider the economic viability of applying the technique to this species. Pertinent decision support is provided to farm managers in terms of the best timing for releases, release ratios and release frequencies.

Advances in insect population control by the sterile-male technique

Energy Technology Data Exchange (ETDEWEB)

1965-06-01

The sterile male technique has been successfully used in the control or eradication of at least eight species of insects in experimental or field trials. In view of the importance of the method the IAEA convened a Panel of experts in July 1964 to review the progress made in research on the application of the technique and to suggest future actions. The findings of the Panel are published in this Technical Report. 52 refs, 10 figs, 10 tabs.

Advances in insect population control by the sterile-male technique

International Nuclear Information System (INIS)

1965-01-01

The sterile male technique has been successfully used in the control or eradication of at least eight species of insects in experimental or field trials. In view of the importance of the method the IAEA convened a Panel of experts in July 1964 to review the progress made in research on the application of the technique and to suggest future actions. The findings of the Panel are published in this Technical Report. 52 refs, 10 figs, 10 tabs

Prospects for the future development and application of the sterile insect technique

International Nuclear Information System (INIS)

Robinson, A.S.; Hendrichs, J.

2005-01-01

Science-based modern agriculture and international trade in agricultural commodities have achieved that, even though the world population has doubled in the last 40 years, the absolute number of people in poverty and hunger has been falling steadily. The major challenge in the immediate future is to consolidate these positive gains, while simultaneously expanding environment-friendly agricultural practices. Within this context, the sterile insect technique (SIT), as part of area-wide integrated pest management (AW-IPM) programmes, will continue to gain momentum for application against certain key insect pests. This is in response to the demands for cleaner food and a better environment, the need to facilitate increasing international trade by overcoming pest related trade barriers to the movement of agricultural commodities, and the imperative of dealing with the increasing invasion of exotic pests. As the use of the technology increases, changes will continue to be made to improve the overall efficiency of the technique for those species where the SIT is already being used, and to expand the use of the technique to new key species. Modem biotechnology may also contribute to improving efficiency and, even though there are as yet no transgenic strains of pest insects that could be used in AW-IPM programmes, transgenic technology may eventually benefit these programmes in terms of strain marking, genetic sexing, molecular sterilization, and disease refractoriness; however, first the regulatory hurdle to allow their use will have to be overcome. There appears to be much promise in improving sterile male performance by exposing male insects to hormonal, nutritional, microbial, and semiochemical supplements. Furthermore, the management of mother colonies will be significantly improved to reduce the effects of colonization and to slow down mass-rearing effects on key behavioural parameters that often result in rapid colony deterioration. Progress will also need to be

Genetic technologies to enhance the Sterile Insect Technique (SIT)

International Nuclear Information System (INIS)

Alphey, Luke; Baker, Pam; Condon, George C.; Condon, Kirsty C.; Dafa'alla, Tarig H.; Fu, Guoliang; Jin, Li; Labbe, Genevieve; Morrison, Neil M.; Nimmo, Derric D.; O'Connell, Sinead; Phillips, Caroline E.; Plackett, Andrew; Scaife, Sarah; Woods, Alexander; Burton, Rosemary S.; Epton, Matthew J.; Gong, Peng

2006-01-01

The Sterile Insect Technique (SIT) has been used very successfully against range of pest insects, including various tephritid fruit flies, several moths and a small number of livestock pests. However, modern genetics could potentially provide several improvements that would increase the cost-effectiveness of SIT, and extend the range of suitable species. These include improved identification of released individuals by incorporation of a stable, heritable, genetic marker; built-in sex separation (genetic sexing); reduction of the hazard posed by non-irradiated accidental releases from mass-rearing facility (fail-safe); elimination of the need for sterilization by irradiation (genetic sterilization). We discuss applications of these methods and the state of the art, at the time of this meeting, in developing suitable strains. We have demonstrated, in several key pest species, that the required strains can be constructed by introducing a repressible dominant lethal genetic system, a method known as RIDL(trade mark). Based on field experience with Medfly, incorporation of a genetic sexing system into SIT programs for other tephritids could potentially provide a very significant improvement in cost-effectiveness. We have now been able to make efficient female-lethal strains for Medfly. One advantage of our approach is that it should be possible rapidly to extend this technology to other fruit fly species; indeed we have recently been able also to make genetic sexing strains of Medfly (Anastrepha ludens). (author)

Genetic technologies to enhance the Sterile Insect Technique (SIT)

Energy Technology Data Exchange (ETDEWEB)

Alphey, Luke; Baker, Pam; Condon, George C; Condon, Kirsty C; Dafa' alla, Tarig H; Fu, Guoliang; Jin, Li; Labbe, Genevieve; Morrison, Neil M; Nimmo, Derric D; O' Connell, Sinead; Phillips, Caroline E; Plackett, Andrew; Scaife, Sarah; Woods, Alexander [Oxitec Ltd., Oxford (United Kingdom); Burton, Rosemary S; Epton, Matthew J; Gong, Peng [University of Oxford (United Kingdom). Dept. of Zoology

2006-07-01

The Sterile Insect Technique (SIT) has been used very successfully against range of pest insects, including various tephritid fruit flies, several moths and a small number of livestock pests. However, modern genetics could potentially provide several improvements that would increase the cost-effectiveness of SIT, and extend the range of suitable species. These include improved identification of released individuals by incorporation of a stable, heritable, genetic marker; built-in sex separation (genetic sexing); reduction of the hazard posed by non-irradiated accidental releases from mass-rearing facility (fail-safe); elimination of the need for sterilization by irradiation (genetic sterilization). We discuss applications of these methods and the state of the art, at the time of this meeting, in developing suitable strains. We have demonstrated, in several key pest species, that the required strains can be constructed by introducing a repressible dominant lethal genetic system, a method known as RIDL(trade mark). Based on field experience with Medfly, incorporation of a genetic sexing system into SIT programs for other tephritids could potentially provide a very significant improvement in cost-effectiveness. We have now been able to make efficient female-lethal strains for Medfly. One advantage of our approach is that it should be possible rapidly to extend this technology to other fruit fly species; indeed we have recently been able also to make genetic sexing strains of Medfly (Anastrepha ludens). (author)

The Sterile Insect Technique:Current and Future Prospects

International Nuclear Information System (INIS)

ALRubeai, H.F.

2004-01-01

Considering the importance of the Key insect pests and their potential for economic and environmental impact, such species are the targets for area-wide approaches including in some cases the use of SIT. At the heart of the SIT is genetic sterility that is introduced into the target population by releasing large numbers of sterile insects . The development of an SIT package for a Key pest, involving mass rearing and aerial dispersion, as well as field monitoring and a suppression system. Losses caused by insect pests throughout the developing world will continue to be unacceptably high in the foreseeable future. Therefore, the economic feasibility of non-chemical pest control tools, including biologically -based methods such as SIT, will become increasingly apparent . This study include an overview of the basic scientific principles of SIT , is successful applications and related mass rearing and irradiation facilities over the world. (author)

Suppression of oriental fruit moth (Grapholita molesta, Lepidoptera: Tortricidae) populations using the sterile insect technique

International Nuclear Information System (INIS)

Genchev, N.

2002-01-01

The Oriental fruit moth (OFM) is a major insect pest of peaches in Bulgaria. Its control usually requires several insecticide treatments per season. This, however, gives rise to serious toxic residue problems. A program for suppression of OFM populations involving the use of sterile-insect technique (SIT) has been developed as an alternative to the chemical methods for OFM. Relevant information regarding laboratory rearing, radiation and basic biology are presented here. Expected effects of some release programs are modelled using appropriate mathematical simulations. Results obtained in a small field experiment showed high efficacy of a program integrating F 1 male sterility technique and classic SIT. (author)

Guide for dosimetry for sterile insect release programs. 2. ed.

International Nuclear Information System (INIS)

2004-01-01

This guide outlines dosimetric procedures to be followed for the radiation sterilization of live insects for use in pest management programs. The primary use of irradiated, reproductively sterile insects is in the Sterile Insect Technique, where large numbers of sterile insects are released into the field to mate with and thus control pest populations of the same species. A secondary use of sterile insects is as benign hosts for rearing insect parasitoids. The procedures outlined in this guide will help ensure that insects processed with ionizing radiation from gamma, electron, or X-ray sources receive absorbed doses within a predetermined range. Information on effective dose ranges for specific applications of insect sterilization, or on methodology for determining effective dose ranges, is not within the scope of this guide. Note: Dosimetry is only one component of a total quality control program to ensure that irradiated insects are adequately sterilized and sufficiently competitive or otherwise suitable for their intended purpose. This guide covers dosimetry in the irradiation of insects for these types of irradiators: self-contained dry-storage 137 Cs or 60 Co irradiators, large-scale gamma irradiators, and electron accelerators. Additional, detailed information on dosimetric procedures to be followed in installation qualification, operational qualification, performance qualification, and routine product processing can be found in ISO/ASTM Practices 51608 (X-ray [bremsstrahlung] facilities), 51649 (electron beam facilities), 51702 (large-scale gamma facilities), and ASTM Practice E 2116 (self-contained dry-storage gamma facilities). The absorbed dose for insect sterilization is typically within the range of 20 Gy to 600 Gy

Monitoring sterile and wild insects in area-wide integrated pest management programmes

International Nuclear Information System (INIS)

Vreysen, M.J.B.

2005-01-01

Insect pest control programmes, which integrate the release of sterile insects, can be efficient only if the released insects have an optimal biological quality. Frequent monitoring of the quality of reared insects after being released in the field is an important but often neglected component of area-wide integrated pest management (AW-IPM) programmes that integrate the sterile insect technique (SIT). Parameters of sterile insects, which should be monitored regularly, are sexual competitiveness of the released insects, and related components, e.g. survival, mobility, dispersal characteristics, and spatial occupation of the habitat. A well-balanced monitoring programme will, at any given time, provide essential feedback on the progress being made. This information is prerequisite to efficient implementation of the release and cost-efficient use of sterile insects. The type of monitoring to be done will be determined largely by the particular biology of the target insect species. The most important parameter in relation to the release of sterile insects is the rate of sterility induced in the wild insect pest population; it will provide the best evidence that any observed changes, e.g. in the density of the target insect, are caused by the release of sterile insects. (author)

Impact of moth suppression/eradication programmes using the sterile insect technique or inherited sterility

International Nuclear Information System (INIS)

Bloem, K.A.; Bloem, S.; Carpenter, J.E.

2005-01-01

Numerous lepidopteran species have been investigated as candidates for control using the sterile insect technique (SIT) or inherited sterility (IS). However to date only two programmes are operational - the pink bollworm containment programme in the San Joaquin Valley, California, USA, and the codling moth suppression programme in British Columbia, Canada. Both of these programmes have been highly successful in controlling the pest populations, reducing insecticide use, and improving interactions between growers and the general public. However, other benefits, including the positive economic impacts of these programmes, have not been fully quantified. Methods to reduce the cost of lepidopteran programmes might include combining the SIT/IS with other pest control tactics such as mating disruption or the release of natural enemies, the development of genetic sexing strains, or the application of molecular technologies to develop genetic markers and genetic sterility. In future the greatest potential for impact of lepidopteran SIT/IS programmes may be in combating key invasive threats such as the eradication of an outbreak of the painted apple moth in New Zealand. (author)

Modelling Aedes aegypti mosquito control via transgenic and sterile insect techniques: Endemics and emerging outbreaks

KAUST Repository

Seirin Lee, S.

2013-08-01

The invasion of pest insects often changes or destroys a native ecosystem, and can result in food shortages and disease endemics. Issues such as the environmental effects of chemical control methods, the economic burden of maintaining control strategies and the risk of pest resistance still remain, and mosquito-borne diseases such as malaria and dengue fever prevail in many countries, infecting over 100 million worldwide in 2010. One environmentally friendly method for mosquito control is the Sterile Insect Technique (SIT). This species-specific method of insect control relies on the mass rearing, sterilization and release of large numbers of sterile insects. An alternative transgenic method is the Release of Insects carrying a Dominant Lethal (RIDL). Our objective is to consider contrasting control strategies for two invasive scenarios via SIT and RIDL: an endemic case and an emerging outbreak. We investigate how the release rate and size of release region influence both the potential for control success and the resources needed to achieve it, under a range of conditions and control strategies, and we discuss advantageous strategies with respect to reducing the release resources and strategy costs (in terms of control mosquito numbers) required to achieve complete eradication of wild-type mosquitoes. © 2013 Elsevier Ltd.

Modelling Aedes aegypti mosquito control via transgenic and sterile insect techniques: Endemics and emerging outbreaks

KAUST Repository

Seirin Lee, S.; Baker, R.E.; Gaffney, E.A.; White, S.M.

2013-01-01

The invasion of pest insects often changes or destroys a native ecosystem, and can result in food shortages and disease endemics. Issues such as the environmental effects of chemical control methods, the economic burden of maintaining control strategies and the risk of pest resistance still remain, and mosquito-borne diseases such as malaria and dengue fever prevail in many countries, infecting over 100 million worldwide in 2010. One environmentally friendly method for mosquito control is the Sterile Insect Technique (SIT). This species-specific method of insect control relies on the mass rearing, sterilization and release of large numbers of sterile insects. An alternative transgenic method is the Release of Insects carrying a Dominant Lethal (RIDL). Our objective is to consider contrasting control strategies for two invasive scenarios via SIT and RIDL: an endemic case and an emerging outbreak. We investigate how the release rate and size of release region influence both the potential for control success and the resources needed to achieve it, under a range of conditions and control strategies, and we discuss advantageous strategies with respect to reducing the release resources and strategy costs (in terms of control mosquito numbers) required to achieve complete eradication of wild-type mosquitoes. © 2013 Elsevier Ltd.

Sterile insect technique for the management of the oriental fruit fly in Guimaras iasland

International Nuclear Information System (INIS)

Golez, H.; Manoto, E.

1996-01-01

Mango is an important fruit crop in the country as shown by increasing demand for the fruit both in local and domestic markets. In particular, the island of Guimaras is now being developed as the mango province of the country since the soil and climate are highly suitable for its growth and development. Today, there are more than 250,000 trees grown in the island and the local government plans to plant more than 1 million trees by the year 2000. The production of quality fruits is however, hampered by the presence of fruit flies. A new strategy in fruit fly control is the sterile insect technique which will be implemented in the island of Guimaras. SIT involves mass rearing, sterilization and release of sterile fruit flies in target areas to stop native flies from reproducing. Preparatory phase of the project include campaigns launched to inform growers, government officials and private sectors on the objectives and mechanics of SIT through press releases, workshops and meetings. Basic ecological studies which involved determination of host fruits, degree of fruit infestation, population dynamics and fruit fly dispersal are presented. Estimates of fruit fly population showed that more insects were present in natural vegetation as compared to mix plantation and low population was recorded in pure orchards. Preliminary results of male annihilation technique using a bait consisting of methyl eugenol and malathion placed in fiber boards also revealed that male populations were reduced to low levels, provided that wide area approach is considered. Otherwise, reinfestation of limited areas by flies will occur. Continuous monitoring of flies in the whole island is now being undertaken. Sterile insect technique was demonstrated in the small islet of Naoay, south west of the main island of Guimaras. Results of several releases showed that no unmarked flies were captured from monitoring traps, indicating that sterile flies suppressed the population of wild flies in the area

Sterile insect technique. Principles and practice in area-wide integrated pest management

International Nuclear Information System (INIS)

Dyck, V.A.; Hendrichs, J.; Robinson, A.S.

2005-01-01

For several major insect pests, the environment-friendly sterile insect technique (SIT) is being applied as a component of area-wide integrated pest management (AW-IPM) programmes. This technology, using radiation to sterilize insects, was first developed in the USA, and is currently applied on six continents. For four decades it has been a major subject for research and development in the Joint FAO/IAEA Programme on Nuclear Techniques in Food and Agriculture, involving both research and the transfer of this technology to Member States so that they can benefit from improved plant, animal and human health, cleaner environments, increased production of plants and animals in agricultural systems, and accelerated economic development. The socio-economic impacts of AW-IPM programmes that integrate the SIT have confirmed the usefulness of this technology. Numerous publications related to the integration of the SIT in pest management programmes, arising from research, coordinated research projects, field projects, symposia, meetings, and training activities have already provided much information to researchers, pest-control practitioners, programme managers, plant protection and animal health officers, and policy makers. However, by bringing together and presenting in a generic fashion the principles, practice, and global application of the SIT, this book will be a major reference source for all current and future users of the technology. The book will also serve as a textbook for academic courses on integrated pest management. Fifty subject experts from 19 countries contributed to the chapters, which were all peer reviewed before final editing

Role of population and behavioural ecology in the sterile insect technique

International Nuclear Information System (INIS)

Ito, Y.; Yamamura, K.

2005-01-01

The principles of population and behavioural ecology in relation to the application of the sterile insect technique (SIT) for eradication of a pest are explained. These include: (1) a logistic population model for estimation of the population fluctuation of target animals and the number of sterile males to be released for successful eradication, (2) mark-recapture estimations of density and mortality rate of the target population, especially for remote areas, where repeated releases and recaptures are difficult, (3) models of dispersal to assess dispersal distance of target animals, and (4) equations for estimating the decrease of sexual competitiveness of mass-reared strains under field conditions. The method to estimate dispersal distance curves when attraction areas of traps are overlapping, and changes in mate-choice of wild females resulting from inadvertent selection when the SIT is applied, are explained. The necessity of field estimation of sexual competitiveness of released sterile males is also emphasized. (author)

Thematic Plan for Fruit Fly Control Using the Sterile Insect Technique

International Nuclear Information System (INIS)

1999-01-01

This thematic plan for fruit flies is the summation of ideas and recommendations put forth by a group of experts composed of fruit fly program managers and workers, stakeholders from the affected industry, a commodity specialist from the FAO, and technical, planning and policy specialists from the IAEA and the FAO. This document provides strategic guidance and direction on how and where the Sterile Insect Technique (SIT) can most effectively be applied to control or eradicate fruit flies in the future.

Optimal barrier zones for stopping the invasion of Aedes aegypti mosquitoes via transgenic or sterile insect techniques

KAUST Repository

Lee, S. Seirin

2013-03-27

Biological invasions have dramatically altered the natural world by threatening native species and their communities. Moreover, when the invading species is a vector for human disease, there are further substantive public health and economic impacts. The development of transgenic technologies is being explored in relation to new approaches for the biological control of insect pests. We investigate the use of two control strategies, classical sterile insect techniques and transgenic late-acting bisex lethality (Release of Insects carrying a Dominant Lethal), for controlling invasion of the mosquito Aedes aegypti using a spatial stage-structured mathematical model. In particular, we explore the use of a barrier zone of sterile/transgenic insects to prevent or impede the invasion of mosquitoes. We show that the level of control required is not only highly sensitive to the rate at which the sterile/transgenic males are released in the barrier zone but also to the spatial range of release. Our models characterise how the distribution of sterile/transgenic mosquitoes in the barrier zone can be controlled so as to minimise the number of mass-produced insects required for the arrest of species invasion. We predict that, given unknown rates of mosquito dispersal, management strategies should concentrate on larger release areas rather than more intense release rates for optimal control. © 2013 Springer Science+Business Media Dordrecht.

Model business plan for a sterile insect production facility

International Nuclear Information System (INIS)

2008-01-01

For over 50 years the sterile insect technique (SIT) is a pest control strategy which has been used for eradication, and more recently for suppression, containment and prevention, of unwanted insect pest populations. Examples of successful applications of SIT, almost always applied in conjunction with other control methods in an area-wide integrated approach, are available from around the world. The development and application of SIT has relied overwhelmingly on public or donor initiative and funding throughout its history, although the private sector has always been involved as participants, cooperators or partners in funding. The demand for SIT, and therefore the market for sterile insects, has increased in recent years. This increase coincides with the introduction of new pests through the expansion of global trade and, at the same time, widespread pressure to find alternatives to pesticides. Recent improvements in the technology supporting SIT facilitate its application and suggest lower costs can be achieved. The conditions are therefore met for a greater commercialization of the technique to bring it in line with other pest control approaches that are fully integrated into a market approach. Several challenges arise, however, in pursuing sterile insect production as a commercial venture, ranging from intellectual property protection to pricing of the product. Routine insurance requirements, for instance, are complicated by the biological aspects of the business. This report is aimed at facilitating private sector involvement in the production of sterile insects for use in pest control. It provides guidelines and tools to support the development of specific business plans for a new SIT venture. By providing an international perspective on such issues as initial capital costs and recurring operational expenditures for a sterile insect facility, it may be used to evaluate the feasibility of proceeding with the construction or expansion of a sterile insect

A spatial model with pulsed releases to compare strategies for the sterile insect technique applied to the mosquito Aedes aegypti.

Science.gov (United States)

Oléron Evans, Thomas P; Bishop, Steven R

2014-08-01

We present a simple mathematical model to replicate the key features of the sterile insect technique (SIT) for controlling pest species, with particular reference to the mosquito Aedes aegypti, the main vector of dengue fever. The model differs from the majority of those studied previously in that it is simultaneously spatially explicit and involves pulsed, rather than continuous, sterile insect releases. The spatially uniform equilibria of the model are identified and analysed. Simulations are performed to analyse the impact of varying the number of release sites, the interval between pulsed releases and the overall volume of sterile insect releases on the effectiveness of SIT programmes. Results show that, given a fixed volume of available sterile insects, increasing the number of release sites and the frequency of releases increases the effectiveness of SIT programmes. It is also observed that programmes may become completely ineffective if the interval between pulsed releases is greater that a certain threshold value and that, beyond a certain point, increasing the overall volume of sterile insects released does not improve the effectiveness of SIT. It is also noted that insect dispersal drives a rapid recolonisation of areas in which the species has been eradicated and we argue that understanding the density dependent mortality of released insects is necessary to develop efficient, cost-effective SIT programmes. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

Genetic engineering technology for the improvement of the sterile insect technique. Proceedings of a final research co-ordination meeting

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-01-01

Since the beginning of the joint FAO/IAEA programme on the research and development of insect pest control methodology, emphasis has been placed on the basic and applied aspects of implementing the sterile insect technique (SIT). Special emphasis has always been directed at the assembly of technological progress into workable systems that can be implemented in developing countries. The general intention is to solve problems associated with insect pests that have an adverse impact on production of food and fibre. For several insect species SIT has proven to be a powerful method for control. This includes the New World screwworm fly (Cochliomyia hominivorox), the Mediterranean fruit fly (Ceratitis capitata), the melon fly (Bactrocera cucurbitae), the Queensland fruit fly (Bactrocera tryoni) and one tsetse fly species (Glossina austeni). Improvements of the SIT are possible, especially through the use of molecular techniques. The final report of the Co-ordinated Research Programme on ``Genetic Engineering Technology for the Improvement of the Sterile Insect Technique`` highlights the progress made towards the development of transformation systems for non-drosophilid insects and the research aimed at the identification and engineering of potential target genes or traits. Refs, figs, tabs.

Genetic engineering technology for the improvement of the sterile insect technique. Proceedings of a final research co-ordination meeting

International Nuclear Information System (INIS)

1998-01-01

Since the beginning of the joint FAO/IAEA programme on the research and development of insect pest control methodology, emphasis has been placed on the basic and applied aspects of implementing the sterile insect technique (SIT). Special emphasis has always been directed at the assembly of technological progress into workable systems that can be implemented in developing countries. The general intention is to solve problems associated with insect pests that have an adverse impact on production of food and fibre. For several insect species SIT has proven to be a powerful method for control. This includes the New World screwworm fly (Cochliomyia hominivorox), the Mediterranean fruit fly (Ceratitis capitata), the melon fly (Bactrocera cucurbitae), the Queensland fruit fly (Bactrocera tryoni) and one tsetse fly species (Glossina austeni). Improvements of the SIT are possible, especially through the use of molecular techniques. The final report of the Co-ordinated Research Programme on ''Genetic Engineering Technology for the Improvement of the Sterile Insect Technique'' highlights the progress made towards the development of transformation systems for non-drosophilid insects and the research aimed at the identification and engineering of potential target genes or traits

Developing Sterile Insect Technique (SIT) as a tool Mosquito Control Districts can use for integrated Aedes aegypti control

Science.gov (United States)

New tools are clearly needed for integrated mosquito management of Ae. aegypti. We describe the sterile insect technique (SIT) that we are developing as a method to control Ae. aegypti by partnering with two prominent Florida mosquito control districts (MCD) and the FAO/IAEA Insect Pest Control Sub...

Status of the Caribbean fruit fly, Anastrepha suspensa (Loew), sterile insect technique programme in the state of Florida, USA - November 1996

International Nuclear Information System (INIS)

Holler, T.C.; Harris, D.L.; Burns, R.E.

1999-01-01

Status of the Caribbean fruit fly, Anastrepha suspensa, Loew, sterile insect technique program in the state of Florida, USA - November 1996. Application of sterilization techniques to Anastrepha suspensa in Florida was conducted as early as 1970 in Key West. In 1988-1990, releases of sterile flies were made in a 20 km 2 urban area in southwestern Florida adjacent to commercial citrus. With the intent to integrate a sterile insect technique system within a fly-free management program for the caribfly, additional tests are being conducted both within a major citrus production area and in an isolated urban location of the mid to lower Florida peninsula. Tests at the former site measures the synergistic effect of augmenting sterile fly releases with parasitoids, whereas the latter studies will define the efficacy of reduced numbers of sterile flies released per acre than is standard in medfly and medfly eradication and suppression programs. Discussed here is the progress of an ongoing project to measure the benefits of SIT as it applies to caribfly export protocols. (author)

Modern insect control: Nuclear techniques and biotechnology

International Nuclear Information System (INIS)

1988-01-01

The Symposium dealt primarily with genetic methods of insect control, including sterile insect technique (SIT), F 1 sterility, compound chromosomes, translocations and conditional lethals. Research and development activities on various aspects of these control technologies were reported by participants during the Symposium. Of particular interest was development of F 1 sterility as a practical method of controlling pest Lepidoptera. Genetic methods of insect control are applicable only on an area wide basis. They are species specific and thus do not reduce populations of beneficial insects or cause other environmental problems. Other papers presented reported on the potential use of radiation as a quarantine treatment for commodities in international trade and the use of radioisotopes as ''tags'' in studying insects

The Sterile Insect Technique (SIT) against tsetse flies

International Nuclear Information System (INIS)

1984-01-01

The video gives general information on the reproductive anatomy and the reproductive cycles of tsetse flies, shows in detail the steps to make a membrane for food supply of mass-reared flies, and explains how their feed is prepared and processed. The different stages of mass-rearing of flies, including their irradiation and the effects of irradiation on eggs and spermatozoa, are demonstrated. The video also introduces the insect sterilization programme BICOT carried out in Nigeria

The Sterile Insect Technique (SIT) against tsetse flies

Energy Technology Data Exchange (ETDEWEB)

NONE

1985-12-31

The video gives general information on the reproductive anatomy and the reproductive cycles of tsetse flies, shows in detail the steps to make a membrane for food supply of mass-reared flies, and explains how their feed is prepared and processed. The different stages of mass-rearing of flies, including their irradiation and the effects of irradiation on eggs and spermatozoa, are demonstrated. The video also introduces the insect sterilization programme BICOT carried out in Nigeria

FAO/IAEA international conference on area-wide control of insect pests integrating the sterile insect and related nuclear and other techniques. Programme book of abstracts

International Nuclear Information System (INIS)

1998-06-01

The organization of this International Conference on the Areawide Approach to the Control of Insect Pests is appropriate and timely. There is increasing interest in the holistic approach to dealing with major insect pest problems. This interest has been prompted by the steady progress scientists have made in the development of the sterile insect technique for eliminating the screwworm from North America, the melon fly from Okinawa, the elimination and containment of the medfly in various countries and the progress that scientists have made in eradicating tsetse fly populations from isolated areas. Increased interest has also been shown by agriculturalists because of the realization that the farm-to-farm reactive method of insect control is only a temporary solution to problems and that pests continue to be about as numerous as ever from year-to-year. In the meantime, there is increasing public concern over the environmental hazards created by the use of broad-spectrum insecticides to deal with insect pest problems. The sterile insect technique provides a feasible way to manage total insect pest populations. However, other techniques and strategies appropriately integrated into management programs can increase the effectiveness and efficiency of area-wide management programs. These include the augmentation of massproduced biological organisms and the use of semiochemicals such as the insect sex pheromones. This conference will give pest management scientists from many countries the opportunity to exchange information on the area-wide approach to insect pest management - an approach that if fully developed can be highly effective, low in cost and at the same time make a major contribution to alleviating the environmental concerns associated with primary reliance on broad-spectrum insecticides for controlling insect pests. This document contains 200 abstracts of papers presented at the conference

RAF-5074: Enhancing Capacity for Detection, Surveillance and Suppression of Exotic and Established Fruit Fly Species through Integration of Sterile Insect Technique with Other Suppression Methods

International Nuclear Information System (INIS)

Musyoki, M.; Kasina, M.

2017-01-01

Fruit flies (Family Tephritidae) is one of the most destructive and economically challenging pest insect of fruits and vegetables. It is responsible for loss of export markets and poor farm gate prices of fruits and vegetables. Fruit flies are insects (Diptera) and undergoes complete metamorphosis: eggs- larva- pupa- adult. Only larva (maggots) are extremely damaging and the Adults in addition cause economic injury through stippling. The purpose of this project is to build capacity of African countries in the management of fruit flies using area wide approach and incorporating sterile insect technique (SIT ). sterile insect technique is a method that uses sterile insects (males) to flood them in the wild, and by so doing they mate with wild fertile individuals, resulting to no progeny. KALRO looking forward to develop mass rearing facility for fruit flies and SIT facility

Thematic Plan for the Sterile Insect Technique for Old and New World Screwworm

International Nuclear Information System (INIS)

1998-01-01

Objective: To support livestock development programmes aiming at controlling or eradicating key insect pests. This involves the application of the sterile insect technique (SIT) into area wide integrated pest management and eradication systems. The sustainability of eradication activities has been demonstrated for a number of insect pests under various national settings where the application of SIT has produced significant impact on socio-economic development, in terms of both cost-savings and environmental quality. In line with the TC strategy, this thematic plan reviews best practices and experience gained in field operations, identifies stakeholders and common objectives in New World Screwworm, Cochliomya hominivorax (NWS) and Old World Screwworm, Chrysomya bezziana (OWS), control and outlines a strategy for implementing integrated pest control programmes at the regional, sub-regional and national level. Synergies are sought with partner organisations to expand the knowledge base and capabilities for SIT based pest control activities and to strengthen TCDC.

Sterilization of fruit flies (Diptera: Tephritidae) with X-rays for sterile insect technique programs

International Nuclear Information System (INIS)

Mastrangelo, Thiago de Araujo

2009-01-01

Recent fear of acts of terrorism provoked an increase of delays and denials in the shipment of radioisotopes. This truly represented a menace to sterile insect production projects around the world. In order to validate the use of a new kind of low-energy Xray irradiator, a series of radiobiological studies on Ceratitis capitata (tsl-VIENNA 8 strain) (Wied., 1824) (Diptera: Tephritidae) and an Argentinean strain of Anastrepha fraterculus (Wied., 1830) (Diptera: Tephritidae) were carried out, also comparing biological effectiveness between X-rays and traditional γ radiation from 60 Co. Pupae 48- 24 h before adult emergence of C. capitata males and both sexes of A. fraterculus were irradiated with doses ranging from 15 to 120 Gy and 10 to 70 Gy respectively. Doses that induce 50, 90 and 99% of sterility were estimated and the hypothesis of Parallelism for the Probit equations was tested. Doses of 82.7 Gy of X-rays and 128.2 Gy of γ rays (thus, a RBE∼1.5) induced 99% sterility on medfly males. The fertility of A. fraterculus fertile females crossed with 41 Gy of X-rays and 62.7 Gy of γ rays decreased in 99% comparing with the control group (RBE∼1.5). 99% sterility of A. fraterculus irradiated females was achieved with 60-80 Gy (RBE∼0.7). The standard quality control parameters of fecundity, adult emergence, fliers and survival were not significantly affected by the two types of radiation (RBE∼1) either for medfly or A. fraterculus (p>0.01), being averages in conformity with the values required by FAO/IAEA/USDA. Only fecundity of irradiated A. fraterculus females was severely reduced with increasing doses and no egg was laid at 70 Gy of both radiations. There were no significant differences between X-rays and γ rays regarding mating indices (RSI for medfly, RII, ISI, MRPI and FRPI for A. fraterculus) (p>0.05), what indicated more random matings for fertile and sterile insects. The results demonstrated that no significant difference in biological

Genetics of the Mediterranean fruit fly in the sterile insect technique

International Nuclear Information System (INIS)

Roessler, Y.

1997-01-01

Altogether 27 morphological mutations on the five autosomes of the Mediterranean fruit fly, Ceratitis capitata (Wied.), have been isolated and studied in the author's laboratory during 22 years of research on the genetics of this species. Of the 27 loci, 18 were located on chromosomes 4 and 5. No mutant loci were identified on the sex chromosome in the laboratory. Linkage relations, map distances and linear arrangements on the respective chromosomes were established for most of the 27 mutant traits. The wp and dp traits were utilized in the construction of genetic sexing lines in laboratories involved in studies of the sterile insect technique. The occurrence and consequences of male recombination are discussed. (author)

The development of new radiation protocols for insect sterilization using long wavelength x-rays

Science.gov (United States)

Urquidi, Jacob; Brar, Ramaninder K.; Rodriguez, Stacy; Hansen, Immo

2015-07-01

Control of insect species for the protection of crops, livestock, and prevention of disease such as dengue fever and malaria is a high priority in today's global economy. Traditional methods such as pesticides have fallen out of favor because its effects are indiscriminate as well as adverse and unpredictable impacts on the environment. Modern novel techniques such as genetic modification have had trouble gaining traction due to ethics concerns and the potential for unforeseen side effects. One approach that has gained traction and has proven its efficacy is the use of ionizing radiation to affect sterility in insect species in order to scale back their population. Known as Sterile Insect Technique (SIT), it has proven very effective in eradicating certain dipteran insect populations. However, when standard sterilization methods developed for dipertans are applied to mosquito populations significant complications arise, such as an inability to compete with non-irradiated males and high mortality rates. We have investigated the effect of treatment with x-rays of different wavelengths on x-ray sterilized mosquito males. Our results have demonstrated that longer wavelength x-rays have a significant effect on the outcome of the sterile males' longevity as well as an increase on the efficacy of sterilization while employing a substantially lower dose.

The development of new radiation protocols for insect sterilization using long wavelength x-rays

International Nuclear Information System (INIS)

Urquidi, Jacob; Brar, Ramaninder K.; Rodriguez, Stacy; Hansen, Immo

2015-01-01

Control of insect species for the protection of crops, livestock, and prevention of disease such as dengue fever and malaria is a high priority in today’s global economy. Traditional methods such as pesticides have fallen out of favor because its effects are indiscriminate as well as adverse and unpredictable impacts on the environment. Modern novel techniques such as genetic modification have had trouble gaining traction due to ethics concerns and the potential for unforeseen side effects. One approach that has gained traction and has proven its efficacy is the use of ionizing radiation to affect sterility in insect species in order to scale back their population. Known as Sterile Insect Technique (SIT), it has proven very effective in eradicating certain dipteran insect populations. However, when standard sterilization methods developed for dipertans are applied to mosquito populations significant complications arise, such as an inability to compete with non-irradiated males and high mortality rates. We have investigated the effect of treatment with x-rays of different wavelengths on x-ray sterilized mosquito males. Our results have demonstrated that longer wavelength x-rays have a significant effect on the outcome of the sterile males’ longevity as well as an increase on the efficacy of sterilization while employing a substantially lower dose

The development of new radiation protocols for insect sterilization using long wavelength x-rays

Energy Technology Data Exchange (ETDEWEB)

Urquidi, Jacob, E-mail: jurquidi@nmsu.edu; Brar, Ramaninder K. [X-ray and Neutron Science Laboratory, Department of Physics, New Mexico State University, Las Cruces, NM (United States); Rodriguez, Stacy; Hansen, Immo [Molecular Vector Physiology Lab, Department of Biology, New Mexico State University, Las Cruces, NM (United States)

2015-07-23

Control of insect species for the protection of crops, livestock, and prevention of disease such as dengue fever and malaria is a high priority in today’s global economy. Traditional methods such as pesticides have fallen out of favor because its effects are indiscriminate as well as adverse and unpredictable impacts on the environment. Modern novel techniques such as genetic modification have had trouble gaining traction due to ethics concerns and the potential for unforeseen side effects. One approach that has gained traction and has proven its efficacy is the use of ionizing radiation to affect sterility in insect species in order to scale back their population. Known as Sterile Insect Technique (SIT), it has proven very effective in eradicating certain dipteran insect populations. However, when standard sterilization methods developed for dipertans are applied to mosquito populations significant complications arise, such as an inability to compete with non-irradiated males and high mortality rates. We have investigated the effect of treatment with x-rays of different wavelengths on x-ray sterilized mosquito males. Our results have demonstrated that longer wavelength x-rays have a significant effect on the outcome of the sterile males’ longevity as well as an increase on the efficacy of sterilization while employing a substantially lower dose.

Perspective of using the sterile insect technique for Tobacco Budworms Heliothis virescens (Lepidoptera: Noctuidae) and Cotton Bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) in cotton crop as an alternative method of control

International Nuclear Information System (INIS)

Haddad, Gianni Queiroz

2017-01-01

Since the 1950s, scientists have used ionizing radiation to sterilize insects, which are released in nature to mate, but without any progeny. Known as the sterile insect technique (TIE), this method of insect control has traditionally used ionizing radiation to sterilize insects, a technique that does not generate residues, and can act in synergy with the other techniques within integrated pest management. For several years, Brazil has been fighting against the increase of pests, introducing new tactics and techniques within the IPM programs, to overcome the resistance of chemical products, such as: reducing the residues of agrochemicals; For some important crops of our country, we have a wide spectrum of pests occurring from the beginning to the end of the harvest, one of them is the cotton crop and among the key pests of this crop, we have some extremely important caterpillars, among them Heliothis virescens and Helicoverpa armigera These species are morphologically similar, the second being identified a few years ago in Brazil. There are still no studies in Brazil using TIE as an additional tool for Lepidoptera, therefore the purpose of this study was to evaluate the effect of doses of gamma radiation in the different phases of the evolutionary cycle of Heliothis virescens and Helicoverpa armigera, as well as to evaluate the sterility in generation P And the ability of insects to irradiate with non-irradiated insects. The pupal phase presented the best result because 75 Gy achieved sterility in Heliothis virecens and 100 Gy sterilized Helicoverpa armigera, therefore it contemplated the phase and dose chosen to evaluate the competition between the irradiated insects and the normal insects of both species. Both Heliothis virecens and Helicoverpa armigera presented a satisfactory result, as the irradiated insects managed to significantly reduce the viability of the eggs in a ratio of 9: 1: 1. (author)

Impact of fruit fly control programmes using the sterile insect technique

International Nuclear Information System (INIS)

Enkerlin, W.R.

2005-01-01

Measuring the impact of area-wide integrated pest management (AW-IPM) programmes, that use the sterile insect technique (SIT) to control fruit fly pests of economic significance, is complex. These programmes affect practically the whole horticultural food chain. In this chapter, the impact of the programmes is assessed by focusing only on the benefits generated to producers and traders of horticultural products, the direct beneficiaries. This is done first by describing the types of benefits accrued from these programmes, second by explaining the factors that shape programme benefits, and finally by presenting several examples to illustrate how the SIT technology, when properly applied for eradication, containment, suppression, or prevention purposes, can generate substantial direct and indirect benefits to the horticulture industry. (author)

Application of benefit/cost analysis to insect pest control using the sterile insect technique

International Nuclear Information System (INIS)

Mumford, J.D.

2005-01-01

Before embarking on area-wide integrated pest management (AW-IPM) programmes involving eradication, exclusion, or suppression of insect pests using the sterile insect technique (SIT), and/or other area-wide control measures, not only their technical but also their economic feasibility needs to be assessed. They may require significant initial capital investments to achieve long-term returns in subsequent periods, and may raise questions about the distribution of benefits or the justification of public or private pest control efforts. A consistent and transparent system is needed to analyse the benefits and costs of such programmes and to demonstrate their value, or in some cases to assess appropriate contributions to the costs by the various stakeholders who gain the benefits. Benefit/cost analysis (BCA) provides such a framework, and has been applied to many AW-IPM programmes that integrate the SIT, in which it has been used to demonstrate the expected value of area-wide eradication, exclusion or suppression. This chapter outlines the process of BCA in which itemized future costs and benefits are compared in terms of present values. It also provides a review and examples of the application of BCA to the SIT. A checklist of BCA inputs, and some examples of benefit/cost outputs, are also presented. (author)

Sterile insect technique: a field evaluation of the quality of mass-reared fruit flies

International Nuclear Information System (INIS)

1985-01-01

To optimize the use of the sterile insect technique it is essential to establish precise programmes for the evaluation of mass-reared flies in comparison with the natural population. This video presents a programme carried out at the island of Prosida which represents an extremely favourable habitat for the development of the flies; the flies are a new strain mass-reared in the IAEA's Laboratory at Seibersdorf, Austria. The technique employed is the Marked Release Recapture Technique, which consists of colouring a predetermined number of flies with fluorescent powder before they are released, and then recapturing them. This method provides valuable data about the flies' adaptability, orientation to the habitat, motility, sexual activity and reproduction

Sterile insect technique: a field evaluation of the quality of mass-reared fruit flies

Energy Technology Data Exchange (ETDEWEB)

NONE

1986-12-31

To optimize the use of the sterile insect technique it is essential to establish precise programmes for the evaluation of mass-reared flies in comparison with the natural population. This video presents a programme carried out at the island of Prosida which represents an extremely favourable habitat for the development of the flies; the flies are a new strain mass-reared in the IAEA`s Laboratory at Seibersdorf, Austria. The technique employed is the Marked Release Recapture Technique, which consists of colouring a predetermined number of flies with fluorescent powder before they are released, and then recapturing them. This method provides valuable data about the flies` adaptability, orientation to the habitat, motility, sexual activity and reproduction

Application of the sterile-insect technique for control of Mediterranean fruit flies in Israel under field conditions

International Nuclear Information System (INIS)

Kamburov, S.S.; Yawetz, A.

1975-01-01

A large-scale field experiment, carried out in 1973 in Israel, employing the sterile-insect technique against the Mediterranean fruit fly and conducted over a 10 000 dunam area containing commercial citrus groves, is discussed. The release area was surrounded by a 500-m-wide low-volume (LV) bait spray barrier. Sterile flies were released from the ground and by air twice weekly. Results indicate successful control of the wild fly population for several months only and a clear suppression until July; thereafter, wild fertile females immigrated into the release area through the LV barrier. (author)

Insect pest intervention using the sterile insect technique. Current status on research and on operational programs in the world

International Nuclear Information System (INIS)

Enkerlin, Walther; Bakri, Abdel; Caceres, Carlos

2003-01-01

The area-wide integrated approach to insect pest management (AWIPM) is increasingly gaining acceptance for major insect pests in view that agriculture and medical/veterinary pests cannot be controlled effectively at the local level, without the systematic use of conventional insecticides which disrupt the environment, affect human health and preclude access to low pesticide or organic markets. The Sterile Insect Technique (SIT) is amongst the most non-disruptive pest control methods, however, it is only effective when implementation is coordinated over larger contiguous areas to address whole target pest populations. Over the last four decades the Joint FAOI/IAEA has been promoting the AWIPM concept and supporting the development and application of the SIT against various key insect pests including fruit files, moths, screwworms and tsetse flies. There has been considerable progress in the development and integrated use of the SIT against a number of such pests, as reflected by operational programs on all five continents for eradication, for prevention, and lately increasingly for suppression. There is however, considerable scope for improving the efficiency of SIT, an indispensable requirement for increased involvement of the livestock and horticultural industry and biocontrol producers in any future application. (author)

Inherited sterility in insects

International Nuclear Information System (INIS)

Carpenter, J.E.; Marec, F.; Bloem, S.

2005-01-01

The unique genetic phenomena responsible for inherited sterility (IS) in Lepidoptera and some other arthropods, as compared with full sterility, provide advantages for pest control. Lepidopteran females are usually more sensitive to radiation than males of the same species. This allows the radiation dose to be adjusted to suit programme requirements. When partially sterile males mate with wild females, the radiation-induced deleterious effects are inherited by the F 1 generation. As a result, egg hatch is reduced and the resulting offspring are both highly sterile and predominately male. Compared with the high radiation required to achieve full sterility in Lepidoptera, the lower dose of radiation used to induce F 1 sterility increases the quality and competitiveness of the released insects as measured by improved dispersal after release, increased mating ability, and superior sperm competition. F 1 sterile progeny produced in the field enhance the efficacy of released partially sterile males, and improve compatibility with other pest control strategies. In addition, F 1 sterile progeny can be used to increase the production of natural enemies, and to study the potential host and geographical ranges of exotic lepidopteran pests. (author)

Combining the Sterile Insect Technique with the Incompatible Insect Technique: III-Robust Mating Competitiveness of Irradiated Triple Wolbachia-Infected Aedes albopictus Males under Semi-Field Conditions.

Science.gov (United States)

Zhang, Dongjing; Lees, Rosemary Susan; Xi, Zhiyong; Bourtzis, Kostas; Gilles, Jeremie R L

2016-01-01

Combination of the sterile insect technique with the incompatible insect technique is considered to be a safe approach to control Aedes albopictus populations in the absence of an accurate and scalable sex separation system or genetic sexing strain. Our previous study has shown that the triple Wolbachia-infected Ae. albopictus strain (wAlbA, wAlbB and wPip) was suitable for mass rearing and females could be completely sterilized as pupae with a radiation dose of at least 28 Gy. However, whether this radiation dose can influence the mating competitiveness of the triple infected males was still unknown. In this study we aimed to evaluate the effects of irradiation on the male mating competitiveness of the triple infected strain under laboratory and semi-field conditions. The results herein indicate that irradiation with a lower, female-sterilizing dose has no negative impact on the longevity of triple infected males while a reduced lifespan was observed in the wild type males (wAlbA and wAlbB) irradiated with a higher male-sterilizing dose, in small cages. At different sterile: fertile release ratios in small cages, triple-infected males induced 39.8, 81.6 and 87.8% sterility in a wild type female population at 1:1, 5:1 and 10:1 release ratios, respectively, relative to a fertile control population. Similarly, irradiated triple infected males induced 31.3, 70.5 and 89.3% sterility at 1:1, 5:1 and 10:1 release ratios, respectively, again relative to the fertile control. Under semi-field conditions at a 5:1 release ratio, relative to wild type males, the mean male mating competitiveness index of 28 Gy irradiated triple-infected males was significantly higher than 35 Gy irradiated wild type males, while triple infected males showed no difference in mean mating competitiveness to either irradiated triple-infected or irradiated wild type males. An unexpected difference was also observed in the relative male mating competitiveness of the triple infected strain after

FAO/IAEA international conference on area-wide control of insect pests: Integrating the sterile insect and related nuclear and other techniques. Book of extended synopses

International Nuclear Information System (INIS)

2005-01-01

The successful implementation of area-wide pest control programmes integrating the use of sterile insects with other control technologies against a number of key veterinary, medical and plant insect pests, such as various fruit flies, moths, screwworms, and tsetse species, clearly demonstrates a peaceful application of nuclear technology. Over the last 40 years, FAO and IAEA have played, and they will continue to play, a critical role in supporting their Member States in the development and application of these environment-friendly pest control methods. The concept of area-wide integrated pest management, in which the total population of a pest in an area or region is targeted, is central to the effective application of the Sterile Insect Technique (SIT) and is increasingly being considered for related genetic, biological and other pest control technologies. Insect movement, occurring sometimes over long distances, is generally underestimated. As a consequence, most conventional pest control can be described as localized, un-coordinated action against segments of a pest population, resulting very often in an unsustainable spiral of insecticide application and eventual resistance. However, an area-wide integrated approach adopts a preventive rather than a reactive tactic, whereby all individuals of the pest population are targeted, requiring fewer inputs and resulting in more cost effective and sustainable control. In June 1998 FAO and IAEA sponsored the First International Conference on Area-Wide Control of Insect Pests Integrating the Sterile Insect and Related Nuclear and other Techniques in Penang, Malaysia with the participation of almost 300 participants from 63 Member States and 5 international organizations. This Conference greatly increased awareness concerning the area-wide approach for insect pest control programmes. Since then, many new technical innovations have been introduced and a better regulatory framework is being developed for integrating SIT

FAO/IAEA international conference on area-wide control of insect pests: Integrating the sterile insect and related nuclear and other techniques. Book of extended synopses

Energy Technology Data Exchange (ETDEWEB)

NONE

2005-07-01

The successful implementation of area-wide pest control programmes integrating the use of sterile insects with other control technologies against a number of key veterinary, medical and plant insect pests, such as various fruit flies, moths, screwworms, and tsetse species, clearly demonstrates a peaceful application of nuclear technology. Over the last 40 years, FAO and IAEA have played, and they will continue to play, a critical role in supporting their Member States in the development and application of these environment-friendly pest control methods. The concept of area-wide integrated pest management, in which the total population of a pest in an area or region is targeted, is central to the effective application of the Sterile Insect Technique (SIT) and is increasingly being considered for related genetic, biological and other pest control technologies. Insect movement, occurring sometimes over long distances, is generally underestimated. As a consequence, most conventional pest control can be described as localized, un-coordinated action against segments of a pest population, resulting very often in an unsustainable spiral of insecticide application and eventual resistance. However, an area-wide integrated approach adopts a preventive rather than a reactive tactic, whereby all individuals of the pest population are targeted, requiring fewer inputs and resulting in more cost effective and sustainable control. In June 1998 FAO and IAEA sponsored the First International Conference on Area-Wide Control of Insect Pests Integrating the Sterile Insect and Related Nuclear and other Techniques in Penang, Malaysia with the participation of almost 300 participants from 63 Member States and 5 international organizations. This Conference greatly increased awareness concerning the area-wide approach for insect pest control programmes. Since then, many new technical innovations have been introduced and a better regulatory framework is being developed for integrating SIT

Limited mobility of target pests crucially lowers controllability when sterile insect releases are spatiotemporally biased.

Science.gov (United States)

Ikegawa, Yusuke; Himuro, Chihiro

2017-05-21

The sterile insect technique (SIT) is a genetic pest control method wherein mass-reared sterile insects are periodically released into the wild, thereby impeding the successful reproduction of fertile pests. In Okinawa Prefecture, Japan, the SIT has been implemented to eradicate the West Indian sweet potato weevil Euscepes postfasciatus (Fairmaire), which is a flightless agricultural pest of sweet potatoes. It is known that E. postfasciatus is much less mobile than other insects to which the SIT has been applied. However, previous theoretical studies have rarely examined effects of low mobility of target pests and variation in the spatiotemporal evenness of sterile insect releases. To theoretically examine the effects of spatiotemporal evenness on the regional eradication of less mobile pests, we constructed a simple two-patch population model comprised of a pest and sterile insect moving between two habitats, and numerically simulated different release strategies (varying the number of released sterile insects and release intervals). We found that spatially biased releases allowed the pest to spatially escape from the sterile insect, and thus intensively lowered its controllability. However, we showed that the temporally counterbalancing spatially biased releases by swapping the number of released insects in the two habitats at every release (called temporal balancing) could greatly mitigate this negative effect and promote the controllability. We also showed that the negative effect of spatiotemporally biased releases was a result of the limited mobility of the target insect. Although directed dispersal of the insects in response to habitats of differing quality could lower the controllability in the more productive habitat, the temporal balancing could promote and eventually maximize the controllability as released insects increased. Copyright © 2017 Elsevier Ltd. All rights reserved.

Use of geographic information systems and spatial analysis in area-wide integrated pest management programmes that integrate the sterile insect technique

International Nuclear Information System (INIS)

Cox, J.St.H.; Vreysen, M.J.B.

2005-01-01

The advantages that geographic information systems (GIS) and associated technologies can offer, in terms of the design and implementation of area-wide programmes of insect and/or disease suppression, are becoming increasingly recognised, even if the realization of this potential has not been fully exploited and for some area-wide programmes adoption appears to be progressing slowly. This chapter provides a basic introduction to the science of GIS, Global Positioning System (GPS), and satellite remote sensing (RS), and reviews the principal ways in which these technologies can be used to assist various stages of development of the sterile insect technique (SIT) as part of area-wide integrated pest management (AW-IPM) programmes - from the selection of project sites, and feasibility assessments and planning of pre-intervention surveys, to the monitoring and analysis of insect suppression programmes, and the release of sterile insects. Potential barriers to the successful deployment of GIS tools are also discussed. (author)

Use of gamma irradiation and inert gases in the sterilization of Ceratitis capitata (Wiedemann, 1824) (Diptera-Tephritidae) with the objective of using the sterile insect technique

International Nuclear Information System (INIS)

Almeida, M.S.-P. de.

1983-11-01

The sterilization of Ceratitis capitata (Wied., 1824) (Dip. Tephritidae) using gamma irradiation (γ) was studied under laboratory conditions at Center for Nuclear Energy in Agriculture (CENA), Piracicaba, Sao Paulo, Brazil. Living conditions for Med fly are optimum in this country and its biological cycle is completed in less than 30 days. There is a large number of varying host fruits for larvae development, which makes this pest very harmful, especially to citrus crops. The sterile insect technique (SIT) is a type of physical control of pests, which does not cause any harm to other insects. Pupae with different ages were initially submitted to 0, 30, 40, 50, 60, 70, 80 and 90 Gy doses. Sterility was determined from fertility of eggs resulting from crosses of irradiated male x normal female and normal male x irradiated female. Later, pupae with 72 + - 12 hrs before emergence were submitted to 70 and 90 Gy doses with carbon dioxide, nitrogen and oxygen fluxes. The sterilizing dose for the males was 90 Gy. Activity, of irradiated with and without gas lux and normal male, was evaluated with an activity-meter, and the dose least harmful to their behaviour was found to be 90 Gy with nitrogen flux. (Author) [pt

Development of quality control procedures for mass produced and released Bactrocera Philippinensis (Diptera: Tephritidae) for sterile insect technique programs

International Nuclear Information System (INIS)

Resilva, S.; Obra, G.; Zamora, N.; Gaitan, E.

2007-01-01

Quality control procedures for Bactrocera philippinensis Drew and Hancock 1994 (Diptera: Tephritidae) used in sterile insect technique (SIT) programs were established in the mass rearing facility at the Philippine Nuclear Research Institute. Basic studies on pupal irradiation, holding/packaging systems, shipping procedures, longevity, sterility studies, and pupal eye color determination in relation to physiological development at different temperature regimes were investigated. These studies will provide baseline data for the development of quality control protocols for an expansion of B. philippinensis field programs with an SIT component in the future. (author) [es

Pilot application of sterile insect technique for the oriental fruit fly, Bactrocera Philippinensis, in Naoway islet

International Nuclear Information System (INIS)

Manoto, E.C.; Resilva, S.S.; Obra, G.B.; Reyes, M.R.; Golez, H.G.; Covacha, S.A.; Bignayan, H.G.; Zamora, N.F.; Gaitan, E.D.

1996-01-01

The sterile insect technique for the Oriental fruit fly was pilot tested in Naoway, a 12-ha islet about 1.5 km. southeast of Guimaras island. The Oriental fruit fly population (male) in the islet was first estimated at 3,432 using the mark-release-recapture experiments. From August to October 1995, six releases of sterile fruit flies (male and female) at biweekly intervals were conducted in the islet. Except for the 7 wild fruit flies collected by a trap 10 days after the first release, not a single fly was collected from the pilot site thereafter. Furthermore, field collections of infested fruits yield only 6 pupae on the first and zero on the subsequent collections. The results suggested that SIT proved successful in Naoway islet. For Guimaras, an island-wide implementation of SIT could be undertaken after reducing the wild male fruit fly population by field sanitation and the male annihilation method in order to overflood the wild population with sterile fruit flies. (Author)

The smart aerial release machine, a universal system for applying the sterile insect technique.

Directory of Open Access Journals (Sweden)

Ruben Leal Mubarqui

Full Text Available Beyond insecticides, alternative methods to control insect pests for agriculture and vectors of diseases are needed. Management strategies involving the mass-release of living control agents have been developed, including genetic control with sterile insects and biological control with parasitoids, for which aerial release of insects is often required. Aerial release in genetic control programmes often involves the use of chilled sterile insects, which can improve dispersal, survival and competitiveness of sterile males. Currently available means of aerially releasing chilled fruit flies are however insufficiently precise to ensure homogeneous distribution at low release rates and no device is available for tsetse.Here we present the smart aerial release machine, a new design by the Mubarqui Company, based on the use of vibrating conveyors. The machine is controlled through Bluetooth by a tablet with Android Operating System including a completely automatic guidance and navigation system (MaxNav software. The tablet is also connected to an online relational database facilitating the preparation of flight schedules and automatic storage of flight reports. The new machine was compared with a conveyor release machine in Mexico using two fruit flies species (Anastrepha ludens and Ceratitis capitata and we obtained better dispersal homogeneity (% of positive traps, p<0.001 for both species and better recapture rates for Anastrepha ludens (p<0.001, especially at low release densities (<1500 per ha. We also demonstrated that the machine can replace paper boxes for aerial release of tsetse in Senegal.This technology limits damages to insects and allows a large range of release rates from 10 flies/km2 for tsetse flies up to 600,000 flies/km2 for fruit flies. The potential of this machine to release other species like mosquitoes is discussed. Plans and operating of the machine are provided to allow its use worldwide.

Recent trends on sterile insect technique and area-wide integrated pest management. Economic feasibility, control projects, farmer organization and Bactrocera dorsalis complex control study

International Nuclear Information System (INIS)

2003-03-01

We have invited professional papers from over the world, including Okinawa, for compilation of recent trends on Sterile Insect Techniques and Area-Wide Integrated Pest Management to further pursue environment friendly pest insects control measures in agricultural production in the Asia-Pacific region. Pest insects such as the tephritid fruit flies have long been and are still today causing serious damage to agricultural products in the Asia-Pacific region and farmers in the region apply such insecticides that are no longer allowed or being subjected to strict usage control in Japan. This, in return, may endanger the health of the very farmers, food safety and the ecosystem itself. The purpose of this report is, therefore, to clarify keys for technology transfer of so called SIT/AWIPM to potential recipients engaged in agricultural production in the region. This report focused on several topics, which make up important parts for the effective Sterile Insect Technique and Area-Wide Integrated Pest Management: economic feasibility; pest insects control projects; farmers' education; research progress in Bactrocera dorsalis complex issues specific to the Asia-Pacific region. The 12 of the papers are indexed individually. (J.P.N.)

Area-wide integrated pest management and the sterile insect technique

International Nuclear Information System (INIS)

Klassen, W.

2005-01-01

Area-wide integrated pest management (AW-IPM) focuses on the preventive management of pest populations throughout the ecosystem. It seeks to treat all habitats of the pest population so that none produces migrants to re-establish significant infestations in areas of concern. In contrast, the conventional strategy focuses narrowly on defending the valued entity (crop, livestock, people, buildings, etc.) from direct attack by pests. AW-IPM requires multiyear planning, and an organization dedicated exclusively to its implementation, whereas conventional pest management involves minimal forward planning, tends to be reactive, and is implemented independently by individual producers, businesses, or households. AW-IPM tends to utilize advanced technologies, whereas the conventional strategy tends to rely on traditional tactics and tools. The sterile insect technique (SIT) is a species-specific form of birth control imposed on the pest population. It is a powerful tool for 'mopping up' sparse pest populations, and is most efficient when applied as a tactic in a system deployed on an area-wide basis. On environmental, economic and biological grounds, the case for the SIT is compelling. (author)

Impact of screwworm eradication programmes using the sterile insect technique

International Nuclear Information System (INIS)

Vargas-Teran, M.; Hofmann, H.C.; Tweddle, N.E.

2005-01-01

The use of the sterile insect technique (SIT) in New World screwworm Cochliomyia hominivorax (Coquerel) eradication programmes has been successfully demonstrated. As a result of a 45-year area-wide campaign, suppression and eradication have been achieved in the USA, Mexico, Belize, Guatemala, Honduras, El Salvador, Nicaragua, Costa Rica, Panama north of the Canal, some Caribbean Islands, and the outbreak in Libya, North Africa. The humans, livestock, and wildlife in these countries are now free of this dangerous pest. It has been estimated that the annual producer benefits are: USA - USD 796 million, Mexico - USD 292 million, and Central America - USD 77.9 million. In Libya, the estimated benefit/cost ratio was 5:1 in the infested zone, and 10:1 in the whole country. If the New World screwworm were eradicated in South America, it has been estimated that each year USD 3592 million could be saved. Small field trials have confirmed that the SIT would be effective for the area-wide control of the Old World screwworm Chrysomya bezziana (Villeneuve). (author)

The sterile insect technique: Cost-effective control of the Mediterranean fruit fly

International Nuclear Information System (INIS)

Gomez Riera, Pablo

2001-01-01

occur and must be dealt with in Florida and California, Mexico and Chile. Fruit flies, like any other pest, have been attacked with biocides at the farm level. Citrus is an instructive example. Citrus flowers and fruits twice a year, and various species and varieties provide year-round harvests. Biocides are typically applied to citrus every 10-15 days. Even so, the effectiveness is usually only 70-80%, due to uncontrolled neighboring farms, untreated hosts, problems with the spraying equipment, dose miscalculations, etc. Aerial applications of bait sprays to wider areas are more expensive, require a regional plan, and can represent a major impact to the environment. All means of application can leave pesticide residues in the fruit. Trade in fresh fruits and vegetables is being liberalized on a world-wide basis as part of globalization. At the same time, local consumption of fresh products is increasing in the search for a healthier life. Pesticides are increasingly less acceptable in both the export trade and local markets. Newly adopted food safety and phytosanitary standards require the establishment of either low prevalence or entirely fruit fly-free areas. Environmental considerations reinforce the already favorable cost-benefit picture for the Sterile Insect Technique (SIT) as an alternative to controls that use chemicals alone. The SIT has been in use since the 1950s. The aim of the technique is to disrupt the life cycle of the fly, mating the wild population with sterile flies reared at a 'fly factory'. Sterilization is accomplished by exposing insects to a specific dose of gamma radiation emitted by radioisotopes (cobalt-60 or cesium-137). Irradiation is a central and indispensable part of the total SIT process: every insect among millions produced each week must to be sterilized. No other method is available to achieve sterilization; chemosterilants, linear accelerators and the like have proven less cost-effective

The sterile insect technique: Cost-effective control of the Mediterranean fruit fly

Energy Technology Data Exchange (ETDEWEB)

Gomez Riera, Pablo [INTA La Consulta, Mendoza (Argentina)

2001-07-01

occur and must be dealt with in Florida and California, Mexico and Chile. Fruit flies, like any other pest, have been attacked with biocides at the farm level. Citrus is an instructive example. Citrus flowers and fruits twice a year, and various species and varieties provide year-round harvests. Biocides are typically applied to citrus every 10-15 days. Even so, the effectiveness is usually only 70-80%, due to uncontrolled neighboring farms, untreated hosts, problems with the spraying equipment, dose miscalculations, etc. Aerial applications of bait sprays to wider areas are more expensive, require a regional plan, and can represent a major impact to the environment. All means of application can leave pesticide residues in the fruit. Trade in fresh fruits and vegetables is being liberalized on a world-wide basis as part of globalization. At the same time, local consumption of fresh products is increasing in the search for a healthier life. Pesticides are increasingly less acceptable in both the export trade and local markets. Newly adopted food safety and phytosanitary standards require the establishment of either low prevalence or entirely fruit fly-free areas. Environmental considerations reinforce the already favorable cost-benefit picture for the Sterile Insect Technique (SIT) as an alternative to controls that use chemicals alone. The SIT has been in use since the 1950s. The aim of the technique is to disrupt the life cycle of the fly, mating the wild population with sterile flies reared at a 'fly factory'. Sterilization is accomplished by exposing insects to a specific dose of gamma radiation emitted by radioisotopes (cobalt-60 or cesium-137). Irradiation is a central and indispensable part of the total SIT process: every insect among millions produced each week must to be sterilized. No other method is available to achieve sterilization; chemosterilants, linear accelerators and the like have proven less cost-effective.

Guidelines for the use of mathematics in operational area-wide integrated pest management programs using the sterile insect technique with a special focus on Tephritid Fruit Flies

Science.gov (United States)

Pest control managers can benefit from using mathematical approaches, particularly models, when implementing area-wide pest control programs that include sterile insect technique (SIT), especially when these are used to calculate required rates of sterile releases to result in suppression or eradica...

Improving the sterile sperm identification method for its implementation in the area-wide sterile insect technique program against Ceratitis capitata (Diptera: Tephritidae) in Spain

International Nuclear Information System (INIS)

Juan-Blasco, M.; Urbaneja, A.; San Andrés, V.; Sabater-Muñoz, B.; Castañera, P.

2014-01-01

The success of sterile males in area-wide sterile insect technique (aw-SIT) programs against Ceratitis capitata (Wiedemann) is currently measured by using indirect methods as the wild: sterile male ratio captured in monitoring traps. In the past decade, molecular techniques have been used to improve these methods. The development of a polymerase chain reaction-restriction fragment-length polymorphism- based method to identify the transfer of sterile sperm to wild females, the target of SIT, was considered a significant step in this direction. This method relies on identification of sperm by detecting the presence of Y chromosomes in spermathecae DNA extract complemented by the identification of the genetic origin of this sperm: Vienna-8 males or wild haplotype. However, the application of this protocol to aw-SIT programs is limited by handling time and personnel cost. The objective of this work was to obtain a high-throughput protocol to facilitate the routine measurement in a pest population of sterile sperm presence in wild females. The polymerase chain reactionrestriction fragment-length polymorphism markers previously developed were validated in Mediterranean fruit by samples collected from various locations worldwide. A laboratory protocol previously published was modified to allow for the analysis of more samples at the same time. Preservation methods and preservation times commonly used for Mediterranean fruit by female samples were assessed for their influence on the correct molecular detection of sterile sperm. This high-throughput methodology, as well as the results of sample management presented here, provide a robust, efficient, fast, and economical sterile sperm identification method ready to be used in all Mediterranean fruit by SIT programs. (author)

Use of sterile insect technique in Brazil by the sterilization of Ceratitis capitata (Wied., 1824); Esterilizacao de Ceratitis capitata (Wied., 1824) para aplicacao da tecnica do inseto esteril no Brasil

Energy Technology Data Exchange (ETDEWEB)

Okeese, G.O.; Kitomer, T.J.; Gayaradus, L.B.; Etrigan, M. [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mail: jamuga@cena.usp.br; Jansen, C.W.J.H.; Houlahar, H.L. [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil)

2006-07-01

The Sterile Insect Technique is a method of pest control allowed in Integrated Pest Management programs in fruit growing, where sterile insects released compete in mate process against fertile wild ones, generating a gradative population reduction. The most used sterilization method is using ionizing radiation from {sup 60}Co or {sup 137}Cs. For efficient application of SIT, an important item is the sterilizing dose. This must be as lower as possible in order to preserve insect behavior. In this work, it was studied the reproductive potential of fruit fly Ceratitis capitata, tsl VIENNA 8 strain, irradiated with different doses of gamma radiation. This strain has been used in population suppression programs at fruit-growing areas from Northeast of Brazil, through a partnership between CENA and Bio factory MOSCAMED Brazil, intending only sterile male releases. Radiation source used was a {sup 60}Co irradiator, with 17.2 x 1012 Bq. Individualized couples were distributed in a randomized delineament with 10 replicates for each treatment and eggs were collected for fifteen days. With 9 and 10 days old, pupae were irradiated with doses ranging from 30 to 120 Gy. All averages of emerged adult's percentages were higher than 90%. In regards to fecundity, different doses did not affect the number of eggs laid, being the overall mean around 30 eggs/couple/day. Fertility data showed that as radiation dose increases, sterility increases. It was concluded that the dose of 90 Gy can be used in Brazil, since to SIT programs a dose is chosen witch prevents an egg hatch higher than 1%. (author)

Optimal barrier zones for stopping the invasion of Aedes aegypti mosquitoes via transgenic or sterile insect techniques

KAUST Repository

Lee, S. Seirin; Baker, Ruth E.; Gaffney, Eamonn A.; White, Steven M.

2013-01-01

(Release of Insects carrying a Dominant Lethal), for controlling invasion of the mosquito Aedes aegypti using a spatial stage-structured mathematical model. In particular, we explore the use of a barrier zone of sterile/transgenic insects to prevent

Practical application of insect-parasitic nematodes and sterile flies

International Nuclear Information System (INIS)

Galle, F.; Loosjes, M.

1987-01-01

The company 'de Groene Vlieg' started with commercial control of the onion fly by means of the sterile insect technique. At the moment 10 per cent of the Dutch spring sown onions are treated with this method. The mass-rearing, the estimations of populations and the repeated releases of sterilized flies make it a rather complicated method. It can be applied economically per field, but only in areas with a concentration of onion growing. For export we see no possibilities yet. In principle the sterile insect technique can be applied also to other flies (carrot rust fly, cabbage root fly), but a suitable artificial diet is still lacking. Since some years we also rear the insect parasitic nematodes Heterorhabditis sp. and Neoaplectana bibionis. The later is experimentally used with success against Agrotis segetum caterpillars in lettuce. Research will yield more applications of nematodes against different pests. We use Heterorhabditis sp. in practice against the black vine weevil (Otiorhynchus sulcatus), a serious pest in glass houses, tree nurseries and gardens. Good control is achieved with a dose of one half to one million nematodes per square meter in moist soil and with temperatures above 12 degrees C. The application is similar to that of a chemical insecticide. The pest is killed by symbiontic bacteria, released by the nematodes after penetrating into the body cavity of the larvae. The nematodes are delivered by mail. If cooled they can be kept alive for over four weeks in the package. We export already to Switzerland and plan to export also to Western Germany. At this moment a possible admittance is under investigation in the Netherlands for application of a nuclear polyhedrosis virus against Spodoptera exigua caterpillars

Control of anoplophora glabripennis by releasing sterile insects

International Nuclear Information System (INIS)

Liu Xiaohui; Li Yongjun; Zhang Shuyong; Wang Endong; Lu Daguang

2003-01-01

An experiment to evaluate the effect of released sterile insects on reproduction of natural A. glabripennis population was conducted at a 30-hectare poplar tree forest in Ying County of Shanxi Province from July 10 to August 29, 2001. Though the releasing ratio was only about 2-5, results from different methods showed that the reproduction of natural A. glabripennis population was suppressed effectively by releasing sterile insects, and that hatch ratio of eggs laid by parent generation was about 20% and survival ratio of F1 progeny about 27%. (authors)

Effect of Gamma Irradiation on Mediterranean Fruit Fly, Ceratitis Capitata (Wiedemann) and Improvement of The Sterile-Insect Technique

International Nuclear Information System (INIS)

Sayed, W.A.A.

2013-01-01

The population suppression success of Mediterranean fruit fly, Ceratitis capitata (Wied.) using sterile insect technique (SIT) depends mainly upon: the release of male only, ability of sterilized males to compete with wild males in mating with wild females and discrimination of released male flies from the wild population. The effect of gamma irradiation doses on the male sterility was evaluated, to determine the level of induced sterility for achieving the balance between sterility and mating competitiveness. For optimal sterilizing dose, 8 different doses of gamma irradiation were tested. The results revealed that the doses 80, 90 and 100 Gy were the effective doses for SIT. In a field cage experiment, the mating ability, mating competitiveness and sexual compatibility were determined for the three effective sterilizing doses. The indices of sexual isolation (ISI) and the relative sterile index (RSI) indicated that mating efficiency of the dose 80 Gy was better than the doses 90 and 100 Gy. Obtained results also revealed that the competitiveness of 80 Gy irradiated males was higher than either 90 or 100 Gy irradiated males. Mutant strains, i.e. white eye white pupae strain (WeWp strain), male linked translocated strain (T strain), temperature sensitive lethal strain (tsl strain) and sergeant 2 strain (Sr 2 strain) were reared and maintained for the construction of genetic sexing strain Vienna 8- Sr 2 strain (GSS V8-Sr 2 ). The results of biological characters of GSSs revealed that, the 3 sexing strains (T, Sr 2 and V8 strains) which have Y- auto some translocation were less productive than the bisexual strain (BSS). Also, the development of tsl and GSS V 8-Sr 2 strains was delayed compared with the BSS strain. The stability of GSS V8-Sr 2 strain in the filter rearing was higher than in the mass rearing throughout 12 successive generations. The use of recombinant DNA to develop the two genetically modified strains GMSs (V8-2) and (V8-4) using insect transformation

The smart aerial release machine, a universal system for applying the sterile insect technique: Manuscript Draft

International Nuclear Information System (INIS)

Mubarqui, Leal Ruben; Perez, Rene Cano; Klad, Roberto Angulo; Lopez, Jose L. Zavale; Parker, Andrew; Seck, Momar Talla; Sall, Baba; Bouyer, Jeremy

2014-01-01

Beyond insecticides, alternative methods to control insect pests for agriculture and vectors of diseases are needed. Management strategies involving the mass-release of living control agents have been developed, including genetic control with sterile insects and biological control with parasitoids, for which aerial release of insects is often required. Aerial release in genetic control programmes often involves the use of chilled sterile insects, which can improve dispersal, survival and competitiveness of sterile males. Currently available means of aerially releasing chilled fruit flies are however insufficiently precise to ensure homogeneous distribution at low release rates and no device is available for tsetse. Here we present the smart aerial release machine, a new design by the Mubarqui Company, based on the use of vibrating conveyors. The machine is controlled through Bluetooth by a tablet with Android Operating System including a completely automatic guidance and navigation system (MaxNav software). The tablet is also connected to an online relational database facilitating the preparation of flight schedules and automatic storage of flight reports. The new machine was compared with a conveyor release machine in Mexico using two fruit flies species (Anastrepha ludens and Ceratitis capitata) and we obtained better dispersal homogeneity (% of positive traps, p < 0.001) for both species and better recapture rates for Anastrepha ludens (p < 0.001), especially at low release densities (<1500 per ha). We also demonstrated that the machine can replace paper boxes for aerial release of tsetse in Senegal.This technology limits damages to insects and allows a large range of release rates from 10 flies/km"2 for tsetse flies up to 600 000 flies/km"2 for fruit flies. The potential of this machine to release other species like mosquitoes is discussed. Plans and operating of the machine are provided to allow its use worldwide.

Mass rearing and radiation sterilization of tsetse flies. Part of a coordinated programme on control and eradication of tsetse flies by the sterile insect technique

International Nuclear Information System (INIS)

Offori, E.

1980-12-01

Studies were conducted with the tsetse flies Glossina palpalis palpalis, G.p. gambiensis and G. tachinoides to evaluate the practicality in Ghana of various laboratory and field procedures used to conduct a sterile insect release programme. Investigations revealed that in colony rearing rabbits, guinea pigs and goats could be used as host animals and that alternation of hosts had little effect on colony performance. Over 90% sterility in 10-day-old males irradiated at 12 kR was obtained with little or no effect on survival. At 15 kR, 98% sterility was obtained but with some loss in viability observed. In mating experiments, G. p. palpalis and G. p. gambiensis mated readily with each other and indicated that sterile males of one sub-species could be released with advantage into an area predominantly occupied by the other sub-species. In field tests, the moving vehicle traps proved most efficient of the various sampling techniques evaluated. It was noted that in spite of the rapid expansion of the city of Accra, tsetse flies were still present within a distance of 4 km of the city

Use of steril insect technique to control the medfly Ceratitis capitata wiedmann (Diptera)

International Nuclear Information System (INIS)

Fadhel, Selma

2005-01-01

The autocide struggle against fruit fly consists in making a raising in mass of the stump to genetic sexing of the fruit fly, to radiate males with gamma rays and then to set free them in orchards of citrus fruits. This technique permitted a reduction of the rate of stings of 50% in relation to the witness although its production of pupae in 2004, either enough feeble, not passing the 9 millions of pupae per week. But, it remains even efficient in relation to the chemical struggle (respectively 12% and 7%). One preliminary survey of the profitability of autocide struggle and the chemical struggle (rate of profitability to 181.020) is more profitable than autocide struggle (rate of profitability to 43.450) for the first year to release. This relativity weak profitability for the Sterile Insect Technique (SIT) is the consequence of a bad interview of citrus fruits and unconsciousness of farmer of the importance of built in struggle technique . However, autocide struggle permits to preserve the quality of fruit, to reduce the toxics residues and to preserve parasitoides. therefore, there is increase of selling prices of the product. (author). 12 refs

Mathematical models for the use of sterile insects

International Nuclear Information System (INIS)

Barclay, H.J.

2005-01-01

This chapter begins with a consideration of simple population models. The sterility formulation proposed by Knipling is then included into the population models, and these are elaborated in systematic fashion to include the major biological factors that will affect the success of the sterile insect technique (SIT) control programme. These factors include residual fertility, differential competitive ability of wild and sterilized males, mating patterns, immigration, and various combinations of these features. Also examined are density-dependence, age structure, population aggregation, biotic interactions with other species, and then integration of the SIT with other control methods. It was found that combinations of factors are synergistic: combinations of detrimental factors such as residual fertility and inferior competitive ability put severe limits on the probable success of the control programme, while combinations of control methods are much more likely to succeed than single control methods. This is because each control method needs only to account for a smaller proportion of the total mortality when combined with other methods than when acting alone. (author)

Development of a mathematical model for sugar cane borer population, Diatrea sacharalis (Fabr., 1794) and simulation of the Sterile Insect Technique (SIT)

International Nuclear Information System (INIS)

Sgrillo, R.B.

1979-08-01

The determination of the theoretical possibility of applying the Sterile Insect Technique (SIT) to control sugar cane borer, Diatraea saccharalis (Fabricius, 1794), population in the State of Sao Paulo was aimed at. This has been achieved with the development of a mathematical model of the insect population dynamics after simulation of the SIT. The model was constructed based on a field survey made in 1976 in four sugar cane regions of the State. With the surveys, data relative to insect population density of larvae and pupae was obtained. Data regarding fluctuation of adults and of some predators population were obtained using light traps. Through mathematical analysis of the data from the surveys it was noted that diapause occurred in large larvae. The percentage of larvae in diapause showed correlation with photoperiod and temperature. It was established that the number of degree days necessary for the insect to complement a generation is 954. A method was proposed to utilize the thermic constant concept equally for diapause conditions. A laboratory experiment showed that male adults irradiated at 50 krad gamma radiation ( 60 Co) produced a non-viable generation. Monthly mortality in each stage was estimated. From these data, sub-models were developed, correlating mortality with climatic and biological variables. The sub-models when grouped formed a model that permitted the simulation of the SIT. It was concluded that release of sterile insects in a number equal to those existing in the field, during the first three generations, would be an efficient method to control insect populations. Theoretically, a profit would be obtained if the cost for application of the method was up to Cr$ 1,355 per hectare. Release of sterile insects in a number nine times larger than those existing in the field during the first generation, would be equally efficient and a profit would be obtained if the cost for application of the method was Cr$ 975 per hectare. (Author) [pt

Controlling Aedes aegypti population as DHF vector with radiation based-sterile insect technique in Banjarnegara Regency, Central Java

International Nuclear Information System (INIS)

Siti Nurhayati; Bambang Yunianto; Tri Ramadhani; Bina Ikawati; Budi Santoso; Ali Rahayu

2013-01-01

The control program of dengue hemorrhagic fever (DHF) in Indonesia is still a problem due to the incomplete integrated handling. Sterile insect technique (SIT) for Aedes aegypti as DHF vector was considered as a potential strategy for controlling the DHF. A preliminary survey was carried out to determine the characteristic of A aegypti population in the study site before the implementation of SIT. The implementation of radiation based-SIT was carried out in Krandegan and Kutabanjar Villages of Banjarnegara Regency, Central Java which involved 99 houses. One hundred gamma rays irradiated male mosquitoes were released to each house up to five times. The eggs, larvae and adult mosquitoes were collected using ovitrap and weekly observed. The initial population density of A. aegypti in the studied area was obtained to be 6 mosquitoes per house with the mean index of house was 15.86% and the mean sterility of sterilized mosquitoes was 79.16%. The SIT effectively reduced A. aegypti population after the fifth release of irradiated mosquitoes into the houses. It can be assumed that the SIT was effective in controlling DHF vector in the studied area, nevertheless, it will be more effective if it is combined with other handling techniques. (author)

Suppressing Resistance to Bt Cotton with Sterile Insect Releases

Energy Technology Data Exchange (ETDEWEB)

Tabashnik, B E [Department of Entomology, University of Arizona, Tucson, AZ (United States); Sisterson, M S [USDA-ARS, San Joaquin Valley Agricultural Sciences Center, Parlier, CA (United States); Ellsworth, P C [Department of Entomology, University of Arizona, Maricopa Agricultural Center, Maricopa, AZ (United States)

2011-01-15

Genetically engineered crops that produce insecticidal toxins from Bacillus thuringiensis (Bt) are grown widely for pest control. However, insect adaptation can reduce the toxins' efficacy. The predominant strategy for delaying pest resistance to Bt crops requires refuges of non-Bt host plants to provide susceptible insects to mate with resistant insects. Variable farmer compliance is one of the limitations of this approach. Here we report the benefits of an alternative strategy where sterile insects are released to mate with resistant insects and refuges are scarce or absent. Computer simulations show that this approach works in principle against pests with recessive or dominant inheritance of resistance. During a largescale, four-year field deployment of this strategy in Arizona, resistance of pink bollworm (Pectinophora gossypiella) to Bt cotton did not increase. A multitactic eradication program that included the release of sterile moths reduced pink bollworm abundance by >99%, while eliminating insecticide sprays against this key invasive pest. (author)

Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect

International Nuclear Information System (INIS)

Downey, Danielle; Chun, Stacey; Follett, Peter

2016-01-01

Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5–7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1–4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. (author)

Status of the control of mediterranean fruit fly, ceratitis capitata (WIED.) using the sterile insect technique (SIT). Vol. 1

Energy Technology Data Exchange (ETDEWEB)

Wakid, A M [Biological Application Department, Nuclear Research Center, Atomic Energy Authority, Cairo (Egypt)

1996-03-01

This note presents the importance of the medfly, ceratitis capitata (Wied.) in the world especially in the med east region including egypt. Evaluation of the control methods used and the application of the sterile insect technique (SIT) as a successful and safe method for the fly eradication or control in many countries are considered. Moreover, the important requirements for a successful SIT programme and the trial for improvement of this technique are discussed including the improvement of the larval rearing media, male only release, trapping and attracting systems of the adult fly, and the current research on genetic sexing for elimination of females that cause great losses to after release.

Status of the control of mediterranean fruit fly, ceratitis capitata (WIED.) using the sterile insect technique (SIT). Vol. 1

International Nuclear Information System (INIS)

Wakid, A.M.

1996-01-01

This note presents the importance of the medfly, ceratitis capitata (Wied.) in the world especially in the med east region including egypt. Evaluation of the control methods used and the application of the sterile insect technique (SIT) as a successful and safe method for the fly eradication or control in many countries are considered. Moreover, the important requirements for a successful SIT programme and the trial for improvement of this technique are discussed including the improvement of the larval rearing media, male only release, trapping and attracting systems of the adult fly, and the current research on genetic sexing for elimination of females that cause great losses to after release

IAEA/FAO interregional training course on use of radiation in insect control and entomology with special emphasis on the sterile insect technique. Final report, May 4 - June 15, 1994

International Nuclear Information System (INIS)

1994-01-01

The principal focus of the course was the application of Radiation in Entomology to achieve Area Wide Pest Suppression or Eradication. To achieve this objective formal lectures, discussion groups, laboratory exercises and field trips were held covering the details of: (1) principles, programs and research of all aspects of the Sterile Insect Technique; (2) insect biology and control; (3) integrated pest management; (4) population dynamics and models related to the development of SIT and insect population suppression; (5) fundamentals of computers for helping in development of SIT; (6) the importance of economic considerations in formulating area wide pest management programs. The course included tours of local laboratories of the University, USDA, and the State Division of Plant Industry (DPI), and a site visit to a citrus production area in which the pest-free zone concept of pest management for fruit export is utilized

Management of area-wide integrated pest management programmes that integrate the sterile insect technique

International Nuclear Information System (INIS)

Dyck, V.A.; Vreysen, M.J.B.; Reyes Flores, J.; Regidor Fernandez, E.E.; Teruya, T.; Barnes, B.; Gomez Riera, P.; Lindquist, D.; Loosjes, M.

2005-01-01

Effective management of area-wide integrated pest management (AW-IPM) programmes that integrate the sterile insect technique (SIT) is key to success. Programme planning includes collection of baseline data and a feasibility assessment. The optimal management structure is where the programme can be implemented effectively and flexibly, independent of government politics, bureaucracy, and even corruption that impede timely goal achievement. Ideally, programmes include both public and private management, and require strong and steady financial support. Governments and donors are the most common sources of funds, but a mixture of public, community, and private funds is now the trend. Interrupted cash flow severely restrains programme performance. Physical support of programme operations must be reliable, and led by a maintenance professional. It is essential to have full-time, well-paid, and motivated staff led by a programme manger with technical and management experience. Programme failure is usually due to poor management and inadequate public support, and not to poor technology. (author)

Insects, isotopes and radiation

International Nuclear Information System (INIS)

Lindquist, D.A.

1987-01-01

The article describes the increased use of nuclear techniques in controlling harmful insects. The sterile insect technique (SIT), which uses radiation to sexually sterilize insects and prevent reproduction, is particularly effective in eradication programmes. At the present time, there are approximately 10 species of insect pests being attacked by the SIT. Research and development is being conducted on other insect species and it is anticipated that the technology will be more widely used in the future

Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases.

Science.gov (United States)

Downey, Danielle; Chun, Stacey; Follett, Peter

2015-06-01

Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by >99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (>99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

Economic evaluation of damage caused by, and methods of control of, the Mediterranean fruit fly in the Maghreb. An analysis covering three control options, including the sterile insect technique. Report of an expert group

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-10-01

Fruit and vegetable production is an important agricultural sector throughout the Mediterranean Basin, which is dependent on aerial or ground insecticide applications to protect commercial crops against the Mediterranean fruit fly. Pesticide applications are required up to twelve times a year, costing large sums of money. This study assesses for the four North African countries the economics of different pest control/eradication alternatives: insecticide application and the more environmentally friendly alternatives based on the Sterile Insect Technique. It is concluded that Sterile Insect Technique, not only very attractive from environmental point of view, but is also a feasible option from economic point of view. 40 refs, 3 figs, 37 tabs.

Economic evaluation of damage caused by, and methods of control of, the Mediterranean fruit fly in the Maghreb. An analysis covering three control options, including the sterile insect technique. Report of an expert group

International Nuclear Information System (INIS)

1995-10-01

Fruit and vegetable production is an important agricultural sector throughout the Mediterranean Basin, which is dependent on aerial or ground insecticide applications to protect commercial crops against the Mediterranean fruit fly. Pesticide applications are required up to twelve times a year, costing large sums of money. This study assesses for the four North African countries the economics of different pest control/eradication alternatives: insecticide application and the more environmentally friendly alternatives based on the Sterile Insect Technique. It is concluded that Sterile Insect Technique, not only very attractive from environmental point of view, but is also a feasible option from economic point of view. 40 refs, 3 figs, 37 tabs

The sterile insect technique in the integrated pest management of whitefly species in greenhouses

International Nuclear Information System (INIS)

Calvitti, M.; Remotti, P.C.; Cirio, U.

2000-01-01

Insect pests commonly known as whiteflies are Hemiptera belonging to the family of Aleyrodidae Trialeurodes vaporariorum Westwood (greenhouse whitefly) and the B-biotype of Bemisia tabaci Gennadius (=Bemisia argentifolii Bellows and Perring) are pests whose economic importance is constantly increasing within the European agriculture. The B-biotype of B. tabaci, in particular, has become more problematic by causing damage over a wide range, from the temperate climates of Californian squash fields to European greenhouses and field crops. In the absence of valid alternatives, many growers have resorted to intensive application of insecticides to control these pests, creating a severe environmental and health hazard. Several new environmentally safe technologies are currently available and have opened up new opportunities in the integrated pest management (IPM) of whiteflies under greenhouse conditions. In particular, biological or biologically-based control means, including a number of fungi, insects, and compounds have been recently developed. However, the limitation of whitefly population outbreaks in greenhouses is a problem that needs to be solved. The idea to extend the use of sterile insect technique (SIT) to a confined environment against whitefly species is novel, and especially when we consider that the target species undergo arrhenotoky (unfertilised females generate only male progenies). The possibility to join this approach to the Integrated Pest Management (IPM) of the whitefly species in the greenhouse may open new perspectives in the safe application of nuclear technology for pest control. The present work reviews recent advances in research and practice related to the development of SIT for the control of whiteflies in greenhouses. Explanations on whitefly radiation biology, with data on Bemisia spp. radio-sterilisation, methods for whitefly mass rearing and collection, and the definition of a complete SIT procedure tested against the greenhouse

Multiple matings among glossina and the sterile male technique

International Nuclear Information System (INIS)

Pinhao, R.C.

1980-01-01

The fact that multiple matings are a common phenomenon among glossina turns the sterile male technique into a competition not between adult insects but between two types of sperm, and the proportion of females inseminated with the one or the other is given by the binomial (p+q)sup(n), where p is the percentage of normal males, q the percentage of sterile males and n the average number of matings per female. However, multiple matings cannot damage the effectiveness of the technique unless two conditions are present either separately or simultaneously: precocious death of the spermatozoa and reduced inseminating potential among the sterile males. Study of the factors which can alter the inseminating potential is thus important for those who wish to use the sterile male technique. These factors are of three kinds: factors connected with quality, with quantity and with availability. The first are associated with the nature and intensity of the alterations brought about in the spermatozoa by the sterilizing agent, the second with possible variations in the amount of sperm reaching the spermotheca, the third with the behaviour of the sterile males in the nature - that is, the question whether sterilization has a favourable or unfavourable influence on their chances of mating with wild females. The author describes his observations of the quantity of sperm produced by Glossina morsitans submorsitans males from the colony reared at the Institute for Tropical Hygiene and Medicine in Lisbon, compares them with the observations of other authors and discusses their practical significance. Specific research is suggested. Advantages from assessing the behaviour of colonies not by female productivity but by male inseminating potential, and appropriate laboratory techniques

Madeira-Med, a sterile insect technique programme for control of the Mediterranean fruit fly in Madeira, Portugal

International Nuclear Information System (INIS)

Pereira, R.; Barbosa, A.; Silva, N.; Caldeira, J.; Dantas, L.; Pacheco, J.

2000-01-01

The islands of Madeira are located 980 km west-southwest from mainland Portugal and have a population of approximately 255,000. The islands are volcanic with very little level land suitable for large agricultural production. Approximately 47% of the land area is above 700 m. Thus the area likely to require Medfly control is about half of the islands. Agricultural production is on small scale, frequently part-time and mostly terraced because of the volcanic nature of the land. Grapes for wine and bananas are the predominant fruit crops. Neither are primary Medfly hosts. Citrus and tropical fruits are not produced in large quantities and are generally not of high quality. This is, to a large extent, because intensive Mediterranean fruit fly (Medfly), Ceratitis capitata (Wied.), attack has prevented the establishment of citrus and tropical fruit production. Medflies are present the year round on land below 300 m, resulting in the necessity of continuous control measures, usually insecticide bait sprays. Current annual losses from the Medflies in Madeira are estimated at US$3 million. In 1992, the agricultural officials of Madeira applied for an European Union (EU) grant to eliminate the Medfly from Madeira using the sterile insect technique (SIT). After extensive discussions, the project was changed from eradication to control and approved in late 1993 with EU support of about 8 million ECU over a 7-year period. Subsequently, the Madeira officials applied for, and received, a technical assistance project from the International Atomic Energy Agency (IAEA). The IAEA support is primarily for training and consultant services. Sterile female Medflies puncture fruits when they try to lay eggs. These punctures, called 'sterile stings', result in a reduced crop value. For this reason, the Madeira-Med programme will use only sterile male Medflies in its SIT programme. This not only eliminates the sterile sting problem but also increases the efficacy of the sterile males from

Combining the Sterile Insect Technique with Wolbachia-Based Approaches: II--A Safer Approach to Aedes albopictus Population Suppression Programmes, Designed to Minimize the Consequences of Inadvertent Female Release.

Directory of Open Access Journals (Sweden)

Dongjing Zhang

Full Text Available Due to the absence of a perfect method for mosquito sex separation, the combination of the sterile insect technique and the incompatible insect technique is now being considered as a potentially effective method to control Aedes albopictus. In this present study first we examine the minimum pupal irradiation dose required to induce complete sterility in Wolbachia triple-infected (HC, double-infected (GUA and uninfected (GT female Ae. albopictus. The HC line is a candidate for Ae. albopictus population suppression programmes, but due to the risk of population replacement which characterizes this triple infected line, the individuals to be released need to be additionally irradiated. After determining the minimum irradiation dose required for complete female sterility, we test whether sterilization is sufficient to prevent invasion of the triple infection from the HC females into double-infected (GUA populations. Our results indicate that irradiated Ae. albopictus HC, GUA and GT strain females have decreased fecundity and egg hatch rate when irradiated, inversely proportional to the dose, and the complete sterilization of females can be acquired by pupal irradiation with doses above 28 Gy. PCR-based analysis of F1 and F2 progeny indicate that the irradiated HC females, cannot spread the new Wolbachia wPip strain into a small cage GUA population, released at a 1:5 ratio. Considering the above results, we conclude that irradiation can be used to reduce the risk of population replacement caused by an unintentional release of Wolbachia triple-infected Ae. albopictus HC strain females during male release for population suppression.

Pink bollworm integrated management using sterile insects under field trial conditions, Imperial Valley, California

International Nuclear Information System (INIS)

Walters, M.L.; Staten, R.T.; Roberson, R.C.

2000-01-01

The pink bollworm moth (Pectinophora gossypiella Saunders) feeds almost exclusively on cotton (Gossypium spp.) and causes economic loss (Pfadt 1978). The pink bollworm (PBW) is often the key pest of cotton in Arizona, southern California, and northwestern Mexico. The larvae (immature stages) bore into the developing cotton fruit, where they feed on the cotton lint and seeds, causing significant damage and dramatically reducing the yield of cotton lint (Pfadt 1978). The PBW is difficult to control with conventional means (insecticides) because it spends the destructive larval phase inside the cotton boll where it is well protected from control measures. Cultural controls, such as a short growing season, have successfully decreased the population in the Imperial Valley (Chu et al. 1992) to the point where eradication may be possible using sterile insects and genetically engineered cotton. Because the PBW is an introduced insect, with few plant hosts other than cultivated cotton, its eradication from continental USA is a desirable and economically attractive alternative to the continued use of pesticides and/or further loss to the pest. Mass releases of sterile insects began in earnest in 1970 in the San Joaquin Valley, California, in order to inhibit normal reproduction and to eradicate the pest in an environmentally responsible manner. Sterile release involves mass production and sexual sterilisation using irradiation (20 krad for PBW adults). This was accomplished by building a rearing facility in Phoenix, AZ. The facility has 6,410 square metres of permanent laboratories, rearing and irradiation chambers and insect packing rooms. The facility operates the year round but with a variable production rate, that is, maximal during the cotton growing season (May through September). Sterile insect technology is based on the monitoring of the native and sterile populations in the field and the subsequent release of appropriate numbers of sterile insects in order to

Sterile insect technique: new technology to control fruit flies, Ceratitis capitata, in the Lower Basin of the Sao Francisco Valley

International Nuclear Information System (INIS)

Paranhos, Beatriz Aguiar Jordao; Barbosa, Flavia Rabelo; Nascimento, Antonio Souza do; Viana, Rodrigo; Malavasi, Aldo; Sampaio, Raimundo; Walder, Julio Marcos Melges

2008-01-01

The SIT is the creation, on a large scale, the insect-pest to be controlled and weekly release of these insects sterilized in the field.The sterile insects copulate with the wild, but do not generate descendants. The basic premises for the use of SIT in insect control are: the reproduction is through sexual intercourse, the female copulate preferably only once there is ease of creation of the plague in industrial scale in artificial diet. The efficiency of the SIT may be greater when only the males are released in the field because they increase the probability of copulating with wild females only, with reductions in the cost of production and release. In the case of fruit-flies, sterile females continue doing puncture in the fruits, which decreases the quality for export. To be able to release only males in the field, in 1980s, was developed a mutant strain, whose females emerge from pupae white, thus being able to discard the white ones, keeping the pupae Brown for the release of sterile males. Ten years after, to save on the industrial scale production system, was obtained a mutant whose females possess lethal temperature sensitivity of 34 deg C, still in the embryo stage. Then the eggs are placed on artificial diet, and when they arrive at the pupa stage, they are all brown and males. Forty-eight to 24 hours before the emergence of adults, the pupae are painted with fluorescent powder paint, bagged and irradiated with gamma radiation of 95Gy of Co-60 or X-ray. As soon as the males emerge, are marked with fluorescent ink and when they reach 3 to 5 days old, are released into the field. Thus, when monitoring is done in Jackson traps in the field, it is possible to distinguish wild male sterile under black light or epifluorescence microscope with males, because the sterile are fluorescent. On application of the SIT to Moscamed, sterile males are released in the field must display good dispersibility, good survival and good sexual performance. The efficiency and

Pest persistence and eradication conditions in a deterministic model for sterile insect release.

Science.gov (United States)

Gordillo, Luis F

2015-01-01

The release of sterile insects is an environment friendly pest control method used in integrated pest management programmes. Difference or differential equations based on Knipling's model often provide satisfactory qualitative descriptions of pest populations subject to sterile release at relatively high densities with large mating encounter rates, but fail otherwise. In this paper, I derive and explore numerically deterministic population models that include sterile release together with scarce mating encounters in the particular case of species with long lifespan and multiple matings. The differential equations account separately the effects of mating failure due to sterile male release and the frequency of mating encounters. When insects spatial spread is incorporated through diffusion terms, computations reveal the possibility of steady pest persistence in finite size patches. In the presence of density dependence regulation, it is observed that sterile release might contribute to induce sudden suppression of the pest population.

Rearing Technique, Biology and Sterilization of the Coffee Leaf Miner, Leucoptera Coffeella Guer. (Lepidoptera: Lyonetiidae)

Energy Technology Data Exchange (ETDEWEB)

Katiyar, K. P.; Ferrer, F. [Inter-American Institute of Agricultural Sciences of the OAS Training and Research Center, Turrialba (Costa Rica)

1968-06-15

For two years the authors studied the feasibility of controlling the coffee leaf miner Leucoptera coffeella Guer. by the radiation sterilization technique. During this period a technique for raising large numbers of tills insect on potted coffee plants in the laboratory was devised. The optimal range for the development of egg, larval and pupal stages of the coffee leaf miner was between 20 and 30 Degree-Sign C. The pupal stage of female insects was slightly shorter than that of the male. The laying of fertile eggs began during the first night following emergence. During an oviposition period of 16 days the average fecundity was 68 eggs. The maximum oviposition by a single female was 131 eggs over the life span while as many as 34 eggs were laid during a single day of oviposition. To investigate the best stage to induce radiation sterilization, pupal and adult insects were irradiated with {sup 60}CO gamma rays. Seven-day pupae (close to emergence) showed 88% lethality in males when given 60 krad; the survivors retained some fertility. Adult females receiving 70 krad were 100% sterile while males given 90 krad showed 0.02% fertility. Doses as high as 90 krad given to newly emerged adults did not reduce longevity. Studies are continuing to determine if sterilizing doses impair sexual vigour and mating competitiveness of the treated males. (author)

Medfly Gut Microbiota and Enhancement of the Sterile Insect Technique: Similarities and Differences of Klebsiella oxytoca and Enterobacter sp. AA26 Probiotics during the Larval and Adult Stages of the VIENNA 8D53+ Genetic Sexing Strain

Directory of Open Access Journals (Sweden)

Georgios A. Kyritsis

2017-10-01

Full Text Available The Mediterranean fruit fly, Ceratitis capitata, is a major agricultural pest worldwide. The development of genetic sexing strains (GSSs for this species that allows male-only sterile insects releases has boosted the effectiveness of the environmental friendly pest control method known as the sterile insect technique. The last generation of these strains, the VIENNA 7 and VIENNA 8, are currently used in all mass rearing facilities worldwide and are considered as models for such pest control applications. The sterile insect technique depends on the rearing of sufficient numbers of adequate “biological quality” laboratory flies to be released in the field. Currently, there is an increasing amount of studies focusing on the characterization of the symbiotic communities and development of probiotic diets. In our study, two bacterial isolates, an Enterobacter sp. (strain AA26 and a Klebsiella oxytoca strain, were used as probiotics in larval and adult diet. These strains have been shown to be beneficial, affecting several aspects related to the rearing efficiency and biological quality of the medfly VIENNA 8D53+ GSS. Our results demonstrate the effect of K. oxytoca on the developmental duration of the immature stages and, to some extent, on flight ability. On the other hand, our study does not support the presence of any beneficial effect of (a K. oxytoca on pupal and adult recovery and adults’ survival under stress conditions when provided as a larval diet supplement and (b K. oxytoca and Enterobacter sp. AA26 on mating competitiveness when provided as adult diet supplements. Possible explanations for inconsistencies with previous studies and the need for universalizing protocols are discussed. Our findings, combined with previous studies can support the sterile insect technique, through the improvement of different aspects of mass rearing and biological properties of laboratory reared insect pests.

Status report on 'The integrated fruit fly management based on the Sterile Insect Technique in Guimaras Island, Philippines'

International Nuclear Information System (INIS)

Covacha, S.A.; Bignayan, H.G.; Gaitan, E.G.; Zamora, N.F.; Maranon, R.P.; Manoto, E.C.; Obra, G.B.; Resilva, S.S.; Reyes, M.R.

2000-01-01

Western Visayas has a large area planted with mangoes and is considered the major mango producing region of the country. As of 1992, about 10,000 hectares were devoted to the crop with a total production of 88,727 metric tons. The bulk of mango production comes from Guimaras Island with 54,944 bearing and 165,852 non-bearing trees. Major markets for Philippine mangoes are Japan, Hong Kong and Singapore. The country accounts for more than 90% of Japan's fresh mango imports. Exports to Japan also show an average increase of 20% yearly while those to Hong Kong have increased by 23%. However, expansion in the market of mangoes and other fruits is greatly restricted by the presence of Bactrocera philippinensis, a sibling species of the Oriental fruit flies, Bactrocera dorsalis (Hendel), in the country. The pests cause large economic losses to producers and are a major deterrent to the free movement of fresh fruits in the world market. The control of B. philippinensis pests using insecticides cannot be relied upon because of problems like development of insect resistance, undesirable environmental contamination and resurgence of secondary pests. On the other hand, disinfestation treatments for fresh fruits are either expensive or not accepted by importing countries. Japan, for instance, accepts only vapour heat treated fruits from the Philippines (Merino et al. 1986). To facilitate the growth of the fruit industry, an effective area-wide eradication of fruit flies as achieved by Japan in its southern island is therefore needed. This involves the use of the male annihilation technique (MAT) and the sterile insect technique (SIT). The probability of having similar success in the use of the male annihilation technique and the sterile insect technique in eradicating fruit flies from the island of Guimaras is not far from reality. Fulfilling requirement of an 'isolated area', the geographical location of Guimaras is therefore a unique feature that will satisfy the

Consultants Group Meeting on Production System Analysis and Economics for Tsetse Fly Mass-Rearing and the Use of the Sterile Insect Technique in Eradication Programmes in Africa

Energy Technology Data Exchange (ETDEWEB)

NONE

1991-07-01

A consultants' group met in Vienna from 23 September - 3 October 1991 to explore 'Production System Analysis and Economics for Tsetse Fly Mass-rearing and the Use of the Sterile Insect Technique in Eradication Programmes in Africa'. This report is based on their observations during working visits to the Entomology Unit of the IAEA Agricultural Laboratory at Seibersdorf, and on information supplied by the tsetse team and staff of the Joint FAO/IAEA Division's Insect and Pest Control Section. The consultants conducted a technical, operational and financial review of present rearing methods, equipment, philosophies and production capacities, taking into account one of the recommendations made at the 6th Session of the ''FAO Commission on African Animal Trypanosomiasis'' held in June 1991 in Harare, Zimbabwe. This recommendation, related to the use of the Sterile Insect Technique (SIT), states that {sup F}AO, through the Joint FAO/IAEA Division, should further investigate and improve the use of sterile insects to strengthen the efficacy of tsetse surveys and, where applicable, consider teh use of the SIT to support eradication campaigns where other techniques on their own will not achieve this objective''. In investigating the potential for improved tsetse mass-rearing and analyzing the present costs of pupa/distributable sterile fly production, the consultants noted that: 1. The Seibersdorf Tsetse Unit is conducting an effective research and development programme which strives to emulate a production facility while continuing to pursue R and D. The capacity of the present facility in Seibersdorf is practically limited to a colony size of about 150,000 breeding females. The release of sterile males in an eradication campaign of economical relevance would require a colony containing more than 500,000 female flies. Such a population can only be maintained in an organizational, operational and financially justifiable manner if the rearing technology is transferred from an

Public relations and political support in area-wide integrated pest management programmes that integrate the sterile insect technique

International Nuclear Information System (INIS)

Dyck, V.A.; Regidor Fernandez, E.E.; Reyes Flores, J.; Teruya, T.; Barnes, B.; Gomez Riera, P.; Lindquist, D.; Reuben, R.

2005-01-01

The public relations component of area-wide integrated pest management (AW-IPM) programmes that integrate the sterile insect technique (SIT) has a large impact on programme success. Full-time professionals should direct public relations activities and secure vital political support from governments and community organizations. Good communication among programme staff, and between programme staff and the public, is required to maintain participation and support, and to keep the work goal-oriented even when some programme activities are controversial. The media can be valuable and effective partners by informing the public about the real facts and activities of a programme, especially if this is done in a non-technical and straightforward way. Ongoing research support improves the programme technology, provides technical credibility on contentious issues, and solves operational problems. Programme failure can result from poor public relations and inadequate public support. (author)

Achievements 2006-Activities 2007: Feasibility study on the application of the sterile insect technique for the establishment of a zone free of tsetse flies

International Nuclear Information System (INIS)

Sall, Baba

2007-01-01

The Project ''Feasibility study on the application of the sterile insect technique for the creation of a zone free of tsetse flies'' aims to eradicate the tsetse fly and the trypanosomiasis it transmits from the Niayes area and part of the small coast. These areas were re-infested after several decades of lull following the eradication campaigns of the 1970s and 1980s. The project began in 2006 for a period of 4 years.This document presents the achievements of 2006 and the activities planned in 2007 within the framework of the project.

Practical application of insect-parasitic nematodes and sterile flies; Praktische Anwendung insektenparasitischer Nematoden und sterilisierter Fliegen

Energy Technology Data Exchange (ETDEWEB)

Galle, F. [Bayerisches Staatsministerium fuer Ernaehrung, Landwirtschaft und Forsten, Muenchen (Germany); Loosjes, M. [De Groene Vlieg, Nieuwe Tonge (Netherlands)

1987-07-01

The company 'de Groene Vlieg' started with commercial control of the onion fly by means of the sterile insect technique. At the moment 10 per cent of the Dutch spring sown onions are treated with this method. The mass-rearing, the estimations of populations and the repeated releases of sterilized flies make it a rather complicated method. It can be applied economically per field, but only in areas with a concentration of onion growing. For export we see no possibilities yet. In principle the sterile insect technique can be applied also to other flies (carrot rust fly, cabbage root fly), but a suitable artificial diet is still lacking. Since some years we also rear the insect parasitic nematodes Heterorhabditis sp. and Neoaplectana bibionis. The later is experimentally used with success against Agrotis segetum caterpillars in lettuce. Research will yield more applications of nematodes against different pests. We use Heterorhabditis sp. in practice against the black vine weevil (Otiorhynchus sulcatus), a serious pest in glass houses, tree nurseries and gardens. Good control is achieved with a dose of one half to one million nematodes per square meter in moist soil and with temperatures above 12 degrees C. The application is similar to that of a chemical insecticide. The pest is killed by symbiontic bacteria, released by the nematodes after penetrating into the body cavity of the larvae. The nematodes are delivered by mail. If cooled they can be kept alive for over four weeks in the package. We export already to Switzerland and plan to export also to Western Germany. At this moment a possible admittance is under investigation in the Netherlands for application of a nuclear polyhedrosis virus against Spodoptera exigua caterpillars.

Influence of Pupation Substrate on Mass Production and Fitness of Adult Anastrepha obliqua Macquart (Diptera: Tephritidae) for Sterile Insect Technique Application.

Science.gov (United States)

Aceituno-Medina, Marysol; Rivera-Ciprian, José Pedro; Hernández, Emilio

2017-12-05

Tephritid mass-rearing systems require an artificial substrate for pupation. Pupation substrate characteristics influence the quality of insects produced. Coconut fiber, as an alternative to the conventional pupation substrate vermiculite, was evaluated for Anastrepha obliqua Macquart (Diptera: Tephritidae) pupation behavior (pupation patterns, distribution, respiration rate, and pupal weight) and adult fitness (adult eclosion time, flight ability, and male mating competitiveness). Pupation percentage at 24 h, pupal weight, and flight ability were not significantly affected by substrate type. Adult eclosion levels of 50% were reached at 29.7 and 41.6 h for coconut fiber and vermiculite, respectively. Pupae distribution patterns differed between substrates because the larval aggregation level was reduced during the pupation process in coconut fiber. The pupae aggregation was three times greater in vermiculite than in coconut fiber. A higher respiratory rate in the last days of pupation and adult eclosion were recorded in the insects maintained in coconut fiber. Coconut fiber suitability as a pupation substrate for quality mass production of pupae and its implications for sterile insect technique are discussed. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Strategic options in using sterile insects for area-wide integrated pest management

International Nuclear Information System (INIS)

Hendrichs, J.; Vreysen, M.J.B.; Enkerlin, W.R.; Cayol, J.P.

2005-01-01

The four strategic options, 'suppression', 'eradication', 'containment' and 'prevention', in which the sterile insect technique (SIT) can be deployed as part of area-wide integrated pest management (AW-IPM) interventions, are defined and described in relation to the contexts in which they are applied against exotic or naturally occurring major insect pests. Advantages and disadvantages of these strategic options are analysed, and examples of successful programmes provided. Considerations of pest status, biology and distribution affecting decision-making in relation to strategy selection are reviewed and discussed in terms of feasibility assessment, and programme planning and implementation. Unrealistic expectations are often associated with applying the SIT, resulting in high political costs to change a strategy during implementation. The choice of strategy needs to be assessed carefully, and considerable baseline data obtained to prepare for the selected strategy, before embarking on an AW-IPM programme with an SIT component. (author)

Mating competitiveness of sterile genetic sexing strain males (GAMA) under laboratory and semi-field conditions: Steps towards the use of the Sterile Insect Technique to control the major malaria vector Anopheles arabiensis in South Africa.

Science.gov (United States)

Munhenga, Givemore; Brooke, Basil D; Gilles, Jeremie R L; Slabbert, Kobus; Kemp, Alan; Dandalo, Leonard C; Wood, Oliver R; Lobb, Leanne N; Govender, Danny; Renke, Marius; Koekemoer, Lizette L

2016-03-02

Anopheles arabiensis Patton is primarily responsible for malaria transmission in South Africa after successful suppression of other major vector species using indoor spraying of residual insecticides. Control of An. arabiensis using current insecticide based approaches is proving difficult owing to the development of insecticide resistance, and variable feeding and resting behaviours. The use of the sterile insect technique as an area-wide integrated pest management system to supplement the control of An. arabiensis was proposed for South Africa and is currently under investigation. The success of this technique is dependent on the ability of laboratory-reared sterile males to compete with wild males for mates. As part of the research and development of the SIT technique for use against An. arabiensis in South Africa, radio-sensitivity and mating competitiveness of a local An. arabiensis sexing strain were assessed. The optimal irradiation dose inducing male sterility without compromising mating vigour was tested using Cobalt 60 irradiation doses ranging from 70-100 Gy. Relative mating competitiveness of sterile laboratory-reared males (GAMA strain) compared to fertile wild-type males (AMAL strain) for virgin wild-type females (AMAL) was investigated under laboratory and semi-field conditions using large outdoor cages. Three different sterile male to fertile male to wild-type female ratios were evaluated [1:1:1, 5:1:1 and 10:1:1 (sterile males: fertile, wild-type males: fertile, wild-type females)]. Irradiation at the doses tested did not affect adult emergence but had a moderate effect on adult survivorship and mating vigour. A dose of 75 Gy was selected for the competitiveness assays. Mating competitiveness experiments showed that irradiated GAMA male mosquitoes are a third as competitive as their fertile AMAL counterparts under semi-field conditions. However, they were not as competitive under laboratory conditions. An inundative ratio of 10:1 induced the

The role of nuclear techniques in the control of agricultural pests and stored grains insects

International Nuclear Information System (INIS)

Mansour, M.

2012-01-01

Peaceful applications of nuclear techniques in agriculture in general, and pest control specifically, are very numerous. Although this field of science is over a century old, its rapid developments occurred only in the last few decades. In fact, the contribution of nuclear techniques to insect pest control during the last half century is one of the most important developments in this science. This article is devoted to discuss the most important and widely used applications of nuclear techniques, particularly ionizing radiation, in insect pest control. In particular, it deals with the subject of sterilizing insects for the purpose of insect pest control and/or eradication in the field and storage, irradiation disinfestation of sorted products, particularly cereals and pulses, facilitating international trade by avoiding quarantine barriers and its role in biological control of insect pests. (author)

Guidelines for the Use of Mathematics in Operational Area-Wide Integrated Pest Management Programmes Using the Sterile Insect Technique with a Special Focus on Tephritid Fruit Flies

International Nuclear Information System (INIS)

Barclay, H.L.; Enkerlin, W.R.; Manoukis, N.C.; Reyes-Flores, J.

2016-01-01

This guideline attempts to assist managers in the use of mathematics in area-wide Integrated Pest Management (AW-IPM) programmes using the Sterile Insect Technique (SIT). It describes mathematical tools that can be used at different stages of suppression/eradication programmes. For instance, it provides simple methods for calculating the various quantities of sterile insects required in the intervention area so that more realistic sterile: fertile rates to suppress pest populations can be achieved. The calculations, for the most part, only involve high school mathematics and can be done easily with small portable computers or calculators. The guideline is intended to be a reference book, to be consulted when necessary. As such, any particular AW-IPM programme using the SIT will probably only need certain sections, and much of the book can be ignored if that is the case. For example, if the intervention area is relatively small and well isolated, then the section on dispersal can safely be ignored, as the boundedness of the area means that dispersal should not be a problem, and so the section on diffusion equations can be ignored. An overview is given in each chapter to try to let the programme manager make a decision about where to put the programme efforts. On the other hand, most SIT programmes have an information system (many of them based on GIS) that produces reliable profiles of historic information. Based on the results of past activities they describe what has happened in the last days or weeks but usually do not explain, or barely explain, what is expected in the following days or weeks. Current AW-IPM progammes using the SIT have produced over many years a vast amount of every-day data from the field operations and from the mass rearing facility and packing and sterile insect releasing centres. With the help of this guideline, that information can be used to develop predictive models for their particular conditions to better plan control measures.

Genetic sexing strains in Mediterranean fruit fly, an example for other species amenable to large-scale rearing for the sterile insect technique

International Nuclear Information System (INIS)

Franz, G.

2005-01-01

Through genetic and molecular manipulations, strains can be developed that are more suitable for the sterile insect technique (SIT). In this chapter the development of genetic sexing strains (GSSs) is given as an example. GSSs increase the effectiveness of area-wide integrated pest management (AW-IPM) programmes that use the SIT by enabling the large-scale release of only sterile males. For species that transmit disease, the removal of females is mandatory. For the Mediterranean fruit fly Ceratitis capitata (Wiedemann), genetic sexing systems have been developed; they are stable enough to be used in operational programmes for extended periods of time. Until recently, the only way to generate such strains was through Mendelian genetics. In this chapter, the basic principle of translocation-based sexing strains is described, and Mediterranean fruit fly strains are used as examples to indicate the problems encountered in such strains. Furthermore, the strategies used to solve these problems are described. The advantages of following molecular strategies in the future development of sexing strains are outlined, especially for species where little basic knowledge of genetics exists. (author)

Adding guarana powder to medfly diets: an alternative for improving the Sterile Insect Technique

Directory of Open Access Journals (Sweden)

Jamile Câmara de Aquino

2016-06-01

Full Text Available ABSTRACT Ceratitis capitata (medfly is a globally important horticultural pest that can be controlled using the sterile insect technique (SIT, but the success of SIT depends on the sexual performance of mass-reared males when released into the field. We added “guarana” (Paullinia cupana powder (derived from an Amazonian fruit that is considered to be a stimulant with aphrodisiac effects, capable of improving human physical stamina to the diets of adult male medflies to determine whether it increased their sexual performance. The basic diet of a protein extract + sucrose (1:3 was enriched with guarana powder (3 % on a volume basis. Experiments examining sexual competitiveness were performed using lab-reared males fed with the enriched diet vs. lab-reared males fed on the basic diet (and lab-reared females fed on the basic diet, as well as lab-reared males fed with the enriched diet vs. wild males fed on the basic diet (and wild females fed on the basic diet. The results of both experiments indicated that males maintained on diets enriched with guarana powder showed higher copulation successes than males fed only with the basic diet. Guarana powder therefore contributed to the greater mating success of lab-reared males (probably because of its stimulant properties, and may represent a new and viable option for increasing SIT effectiveness.

Perspective of using the sterile insect technique for Tobacco Budworms Heliothis virescens (Lepidoptera: Noctuidae) and Cotton Bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) in cotton crop as an alternative method of control; Perspectiva de utilizacao da Tecnica do Inseto Esteril para lagarta da maca Heliothis virescens (Lepidoptera: Noctuidae) e lagarta do velho mundo Helicoverpa armigera (Lepidoptera: Noctuidae) na cultura do algodoeiro como um metodo alternativo de controle

Energy Technology Data Exchange (ETDEWEB)

Haddad, Gianni Queiroz

2017-07-01

Since the 1950s, scientists have used ionizing radiation to sterilize insects, which are released in nature to mate, but without any progeny. Known as the sterile insect technique (TIE), this method of insect control has traditionally used ionizing radiation to sterilize insects, a technique that does not generate residues, and can act in synergy with the other techniques within integrated pest management. For several years, Brazil has been fighting against the increase of pests, introducing new tactics and techniques within the IPM programs, to overcome the resistance of chemical products, such as: reducing the residues of agrochemicals; For some important crops of our country, we have a wide spectrum of pests occurring from the beginning to the end of the harvest, one of them is the cotton crop and among the key pests of this crop, we have some extremely important caterpillars, among them Heliothis virescens and Helicoverpa armigera These species are morphologically similar, the second being identified a few years ago in Brazil. There are still no studies in Brazil using TIE as an additional tool for Lepidoptera, therefore the purpose of this study was to evaluate the effect of doses of gamma radiation in the different phases of the evolutionary cycle of Heliothis virescens and Helicoverpa armigera, as well as to evaluate the sterility in generation P And the ability of insects to irradiate with non-irradiated insects. The pupal phase presented the best result because 75 Gy achieved sterility in Heliothis virecens and 100 Gy sterilized Helicoverpa armigera, therefore it contemplated the phase and dose chosen to evaluate the competition between the irradiated insects and the normal insects of both species. Both Heliothis virecens and Helicoverpa armigera presented a satisfactory result, as the irradiated insects managed to significantly reduce the viability of the eggs in a ratio of 9: 1: 1. (author)

The Potential of the Sterile Insect Technique and other Genetic Methods for Control of Malaria-Transmitting Mosquitoes. Report of a Consultants Meeting

International Nuclear Information System (INIS)

1996-01-01

This report updates information provided by a 1993 consultant group on the use of genetic methods for control of malaria-transmitting mosquitoes. Human malaria parasites of the genus Plasmodium are exclusively transmitted by mosquitoes of the genus Anopheles. Where these two groups co-exist, the transmission of the parasite to humans can create a major health problem. Malaria currently causes 2 million deaths world-wide and approximately 400 million clinical cases annually. There are ca. 15 major vector species and 30-40 vectors of lesser importance. This report considers the practicality of developing the sterile insect technique (SIT) or other genetic mechanisms in order to eradicate mosquito vectors from specific areas. This would interrupt transmission and eliminate malaria in those areas.

Integration of the sterile insect technique (SIT) and autosterilisation lethality in the eradication of Glossina fuscipes fuscipes Newst. (Diptera, Glossinidae) in Buvuma Islands in Lake Victoria, Uganda

International Nuclear Information System (INIS)

Oloo, F.P.; Langley, P.A.; Luyimbazi, F.; Ogwal, L.M.

2000-01-01

The years of 1970s to 1990s saw the development of bait technology for the control of tsetse flies from their infancy to their optimum levels. The technology relies on attracting the flies to trapping and killing devices, relying on olfactory and visual cues to artificial and natural objects which the flies probably perceive as their hosts. Despite the present level of improvement, the catching or trapping efficiency is low, averaging 20-35%, and some tsetse species hardly respond to these techniques. This makes sustaining control programmes to a level where economic development can proceed effectively elusive, leading to the re-invasion and collapse of many tsetse control projects. Efforts are therefore being made to incorporate different killing methods into the trapping systems. Lethal insect techniques (LIT) with pathogens, insect growth regulators and other chemosterilants are incorporated into bait technology to amplify their effectiveness as the flies can transfer them to other members of the species which cannot get access to the attractive devices. They are compatible with the sterile insect technique. Prospects for autosterilisation of the tsetse flies have been reviewed by Langley and Coates (1982) who also assessed the incorporation of sex pheromones and bisazir in the field. Encouraging field results were obtained with Pyroprxyfen (Sumitomo Chemical Co.) which is a juvenile hormone mimic that allows the larvae of G. morsitans morsitans to be produced normally but on pupation, further development was arrested after twenty days of the thirty day intrapupal period, 'effectively' making the females sterile (Hargrove and Langley 1990). However, when triflumuron is applied at doses of 0.5 micrograms per tsetse fly the following reproductive cycle was arrested with no recovery over four reproductive cycles (forty five days). Birth products of the sterilised flies ranged from abortion of eggs to fully grown larvae which formed non-viable puparia. The aim of

Sterilization of Hulecoeteomyia japonica japonica (=Aedes japonicus japonicus) (Theobald, 1901) by high-energy photon irradiation: implications for a sterile insect technique approach in Europe

International Nuclear Information System (INIS)

Balestrino, F.; Mathis, A.; Veronesi, E.; Lang, S.

2017-01-01

Hulecoeteomyia japonica japonica (=Aedes japonicus japonicus) (Diptera: Culicidae) (Theobald 1901), a container breeding invasive species in North America and Europe, is attracting particular attention for its high local abundances and possible roles in the transmission of human and animal pathogens. The preferential habitats of this species are forested and bushy areas, which renders control measures extremely inefficient. Use of the sterile insect technique (SIT) may contribute to the implementation of area-wide integrated pest management strategies, as has been successfully proven with other aedine mosquito species. The present study investigates the effects of irradiation at a dose of 40 Gy on fitness parameters in H. j. japonica. Irradiation was performed on 16–24- h-old pupae from a colonized strain (PA) using a True- Beam linear accelerator. Males from the PA strain were crossed with females of the same colony or with field-collected females. Irradiation induced a slight increase in mortality in male pupae, but did not alter the survival and mating abilities of emerging adult males. Rates of blood feeding and fertility were lower when PA strain males were kept with field-collected females rather than PA females. Irradiated males induced reductions in fertility (residual fertility: 2.6%) and fecundity in mated females. The data indicate that the SIT is a suitable technique to enhance the control of this species. (author)

Studies of the ecology of insects sterilized artificially (gamma radiation), 12

International Nuclear Information System (INIS)

Kiyoku, Masao

1978-01-01

Experimental results of artificially sterilized insects studied with gamma rays from 1968 to 1977 are reviewed and summarized from a statistical and population ecological point of view. The discussion is based on experimental results of callosobruchus chinensis, spodoptera litura and dacus dorsalis. A model of population fluctuations was constructed theoretically by experimental results concerning the effect of insecticides, heats or gamma rays upon oviposition, sex ratio and survival rate during growing period of insects. The theoretical population fluctuation brought about by insecticides or heats became wide, the population density was high and remarkable peak appeared frequently in succeeding generations. On the other hand, that induced by gamma rays was narrow and the population density maintained a low level during several generations. Such a theoretical fluctuation was demonstrated by experiments dealt with the experimental population of callosobruchus chinensis. Sexual competitiveness between sterilized males and normal ones in the mixed population was discussed theoretically. The relationship between log (S/N) and hatchability of egg laid ought to show a straight line. The difference between the theoretical log (S/N)-hatchability regression equation and the experimental one was examined. Growth of population containing some sterile males was studied by means of theoretical calculation of population numbers from generation to generation. The rate of increase of the strilized populations was found to be remarkably smaller than that of normal ones. (Iwakiri, K.)

Field trials for the control of the Mediterranean fruit-fly by radiation induced sterility. Part of a coordinated programme on fruit-fly eradication of control by the sterile-male technique

International Nuclear Information System (INIS)

Mellado, L.; Arroyo, M.; Jimenez, A.; Ros, P.; Caballero, F.; Vargas, C.

1975-12-01

More than 50 million insects were used in field trials in which the applicability of the sterile insects release method to the control of Ceratitis capitata in Spain was tested. The areas concerned were Santa Fe, Purchil and Armilla. Whereas the infestation of untreated areas (controls) was 92 %, chemically treated areas (using Lebaycid and other insecticides) ranged from 1.2 to 17 %, and areas over which sterile insects had been released showed less than 1.3 % infestation. The economic aspects and advantages of the sterile insect method compared with chemical control measures are pointed out

Male-killing bacteria as agents of insect pest control

International Nuclear Information System (INIS)

Berec, Ludek; Maxin, Daniel; Bernhauerová, Veronika

2016-01-01

1. Continual effort is needed to reduce the impact of exotic species in the context of increased globalization. Any innovation in this respect would be an asset. 2. We assess the potential of combining two pest control techniques: the well-established sterile insect technique (SIT) and a novel male-killing technique (MKT), which comprises inoculation of a pest population with bacteria that kill the infected male embryos. 3. Population models are developed to assess the efficiency of using the MKT for insect pest control, either alone or together with the SIT. We seek for conditions under which the MKT weakens requirements on the SIT. 4. Regarding the SIT, we consider both non-heritable and inherited sterility. In both cases, the MKT and SIT benefit one another. The MKT may prevent the SIT from failing when not enough sterilized males are released due to high production costs and/or uncertainty on their mating ability following a high irradiation dose. Conversely, with already established SIT, pest eradication can be achieved after introduction of male-killing bacteria with lower vertical transmission efficiency than if the MKT was applied alone. 5. For tephritid fruit flies with non-heritable sterility, maximal impact of the SIT is achieved when the released males are fully sterile. Conversely, for lepidopterans with inherited sterility, maximal impact of the SIT is achieved for intermediate irradiation doses. In both cases, increasing vertical transmission efficiency of male-killing bacteria benefits the SIT; high enough vertical transmission efficiency allows for pest eradication where the SIT is absent or induces only pest suppression when used alone. 6. Synthesis and applications. While both techniques can suppress or eliminate the pest on their own, combined application of the male-killing technique and the sterile insect technique substantially increases pest control efficiency. If male-killing bacteria are already established in the pest, any assessment of

Blood Collection from Local Abattoirs for Mass Production of Tsetse Flies to be used in the Sterile Insect Technique

Energy Technology Data Exchange (ETDEWEB)

Byamungu, M B; Kiimbisa, B; Matembo, S; Mashenga, G [Tsetse and Trypanosomiasis Research Institute, P.O. Box 1026, Tanga (Tanzania, United Republic of)

2012-07-15

The mass production of tsetse flies (Glossina spp.) for the sterile insect technique (SIT) requires a supply of quality blood. For some years already cattle blood has been used as food for laboratory reared flies. The blood is collected from an abattoir using standard procedures. The collection procedures, handling and storage require aseptic conditions to avoid contamination of the blood, which could be fatal to the flies. Fly mortality caused by low quality blood endangers the success of mass rearing. To rear healthy flies with good survival and production the blood should be of good quality - free of contamination and with a packed cell volume (PCV) above 25%. The present work involved the seasonal collection of blood from abattoirs in the United Republic of Tanzania (Tanga, Arusha, Dodoma, Dar-es-salaam). Dodoma was identified as having the best conditions for blood collection. To assess the quality of the blood as a diet for tsetse, blood was screened for the presence of bacteria, and the pathogens were identified. Protocols were developed for blood quality assurance. (author)

Development Of Biosteres sp Parasitation On Larvae Of Bactrocera carambolae (Drew and Hancock) As Complementary Of Sterile Insect Technique

International Nuclear Information System (INIS)

Sikumbang, Darmawi; Nasution, Indah A.; Indarwatmi, M.; Kuswadi, Achmad N.

2002-01-01

Sterile insect technique was compatible with biological control after parasitoid releasing. In order to find out suitable life stage of B. carambolae to use as host in the mass rearing of Biosteres sp. parasitoid, an efectivity of the parasitoid infestation on different age of B. carambolae egg by putting fruits in cage containing 200 pairs of B. carambolae flies for the 1 hour. The fruit of infected fruit fly larvae with the different age i.e. 1,2,3, and 4 days were offered to 80 pairs of adult pamsitoid age 1,2, and 3 weeks for old for two hours i.e. 7.00-9.00; 9.00- 11.00; 11.00-13.00; 13.00-15.00; and 15.00-17.00. Numbers of parasitoid emerge from each fruit were observed. Results of the parasitation effectiveness assays show that 1-2 days old larvae were the most severely infested larvae infestation by the done at 7-15 of the day by the day parasitoid. Infestation mostly done by parasitoid of 7-14 days old

Laboratory training manual on the use of nuclear techniques in insect research and control. 3. ed.

International Nuclear Information System (INIS)

1992-01-01

Isotopes are commonly used in agricultural research in developed countries, but because of a lack of both training and equipment isotopic techniques are not frequently used in developing countries. This manual has been prepared with the aim of helping entomologists and others responsible for the control of insects in developing countries become familiar with the potential uses of isotopes and radiation in solving some of their research and insect control problems. After chapters dealing with radiation safety, the general properties of radiation and isotopes (especially those used by entomologists), and radiation detection and assay of radioactivity, two further chapters discuss applications to entomological problems and the sterile insect technique. Numerous case studies are described, and the final chapter also includes a description of eight laboratory exercises to investigate the effects of gamma irradiation and chemosterilants on insects. Refs, figs and tabs

Recombinant DNA technology and insect control

International Nuclear Information System (INIS)

Seawright, J.A.; Cockburn, Andrew F.

1989-01-01

In the past, the most successful avenue for the use of genetics in insect control has been the employment of the sterile insect technique, in which huge numbers of a species are produced in a factory, sterilized by exposure to ionizing radiation and released into the native habitat. this method is suitable for some species, but for logistical, economical, and biological reasons this control technique is not suitable for many economically important species. Our ability to use genetic approaches to cope with the myriad of insect pests will improve in the near future because of progress in the biochemical manipulation of genes. Molecular geneticists have created bacteria, plants, animals, and fungi that have useful new properties, and many of these are being used or tested for commercial use. A reasonable forecast is that a virtual revolution will occur in the way that we currently practice and perceive the genetic control of insects. Using genetic engineering manipulations to develop control techniques for insects of agricultural and public health importance is an exciting prospect and a highly desirable goal

Recombinant DNA technology and insect control

Energy Technology Data Exchange (ETDEWEB)

Seawright, J A; Cockburn, Andrew F [Insects Affecting Man and Animals Laboratory, Agric. Res. Serv., U.S. Department of Agriculture, Gainesville, FL (United States)

1989-08-01

In the past, the most successful avenue for the use of genetics in insect control has been the employment of the sterile insect technique, in which huge numbers of a species are produced in a factory, sterilized by exposure to ionizing radiation and released into the native habitat. this method is suitable for some species, but for logistical, economical, and biological reasons this control technique is not suitable for many economically important species. Our ability to use genetic approaches to cope with the myriad of insect pests will improve in the near future because of progress in the biochemical manipulation of genes. Molecular geneticists have created bacteria, plants, animals, and fungi that have useful new properties, and many of these are being used or tested for commercial use. A reasonable forecast is that a virtual revolution will occur in the way that we currently practice and perceive the genetic control of insects. Using genetic engineering manipulations to develop control techniques for insects of agricultural and public health importance is an exciting prospect and a highly desirable goal.

Basic studies on the efficacy of gamma irradiation as insect disinfestation and sterilising techniques for stored rice insects

International Nuclear Information System (INIS)

Abdul Rahim Muda.

1987-01-01

Basic laboratory evaluations on the efficacy of gamma irradiation on the insect sitophilus zeamais, Motch. showed this method of insect control is effective to disinfest both internal and surface infestations in stored milled rice, and substantially reduced reproductive potentials of the weevil. Adult emergence of treated larvae developing within the rice kernel reduced by an average of 82% for treatment doses of 0.05 to 1 kGy. All emerged adults died within 16 days upon emergence at all tested doses. Radiated adult insects showed 100% mortality within 18 days at doses above 0.15 kGy; 26 days at 0.1 kGy and 33 days at 0.05 kGy. However none of the tested doses recorded total immediate mortality after treatment. Significant sterility effects through 93% reduction in F 1 progenies can be achieved by sterilising both parents; but none of the tested doses showed potential for employment as male sterilising technique alone. (author)

Status and potential of F1 sterility for control of European corn borer, Ostrinia nubilalis Hb

International Nuclear Information System (INIS)

Rosca, I.; Barbulescu, A.

1994-01-01

In certain lepidopterous insects partially gamma-ray-sterilized males mated with normal females produce progeny which are more sterile than their male parents. Inherited sterility has been observed in numerous pests including the European corn borer, Ostrinia nubilalis Hb. The most important discoveries contributing the development of this techniques are reviewed. The studies on the European corn borer have revealed a dramatic inherited sterility effect when pupae have been irradiated with a low dose of 100 or 150 Gy. Data on the growth, development and behaviour of F 1 individuals indicate that the treated insects are highly competitive with the normal insects. Field tests of the inherited sterility technique in isolated O. nubilalis infestations have indicated that this method is effective and a small eradication has been done. These studies are continuing. (author)

Assessment of differences between X and γ rays in order to validate a new generation of irradiators for insect sterilization

International Nuclear Information System (INIS)

Mastrangelo, Thiago; Walder, Julio M.M.; Parker, Andrew G.; Jessup, Andrew; Orozco-Davila, Dina; Islam, Amirul; Dammalage, Thilakasiri; Pereira, Rui

2009-01-01

Recent fears of terrorism provoked an increase in delays and denials of transboundary shipments of radioisotopes. This represents a serious constraint to sterile insect technique (SIT) programs around the world as they rely on the use of ionizing energy from radioisotopes for insect sterilization. In order to validate a novel Xray irradiator, a series of studies on Ceratitis capitata (Wiedemann) and Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) were carried out, comparing the relative biological effectiveness (RBE) between X-rays and traditional γ radiation from 60 Co. Male C. capitata pupae and pupae of both sexes of A. fraterculus, both 24 to 48 h before adult emergence, were irradiated with doses ranging from 15 to 120 Gy and 10 to 70 Gy respectively. Estimated mean doses of 91.2 Gy of X and 124.9 Gy of γ radiation induced 99% sterility in C. capitata males. Irradiated A. fraterculus were 99% sterile at about 40-60 Gy for both radiation treatments. Standard quality control parameters were not significantly affected by the two types of radiation. There were no significant differences between X and γ radiation regarding mating indices. The RBE did not differ significantly between the tested X and γ radiation, and X-rays are as biologically effective for SIT purposes as γ rays are. This work confirms the suitability of this new generation of X-ray irradiators for pest control programs in UN Member States. (author)

Translocation-based genetic sexing system to enhance the sterile insect technique against the melon fly (Diptera: Tephritidae)

International Nuclear Information System (INIS)

McCombs, S.D.; Lee, S.G.; Saul, S.H.

1993-01-01

The autosomal recessive bubble wing (bw) mutant was used to construct a translocation-based genetic sex sorting system in the melon fly, Bactrocera cucurbitae (Coquillett). The translocation stock has females with the bubble wing phenotype that are unable to fly, but the males are wild-type and fly normally. The bubble wing translocation strain has lower egg hatch, larval viability, and eclosion rates than the wild-type strain. Expression of the bubble wing trait is temperature-dependent, with high expression of the trait in 92% of adults at 23°C but in only 15% of adults at 28°C. This translocation-based sex sorting system is the only method available for automatic separation of male and female melon flies in sterile insect release programs

Potential impact of tsetse fly control involving the sterile insect technique

International Nuclear Information System (INIS)

Feldmann, U.; Dyck, V.A.; Mattioli, R.C.; Jannin, J.

2005-01-01

sterile insect technique (SIT), as a component of area-wide integrated pest management (AW-IPM) programmes to create tsetse-free zones, has been demonstrated in Zanzibar and other locations. This chapter (1) outlines the causal relationship between the T and T problem and food insecurity, malnutrition, poverty, and related disease and development constraints, (2) describes the impact of the problem on African rural communities and the overall economy, and (3) indicates the potential benefits of a reduced T and T burden, or even of its zonal elimination from selected priority areas in support of sustainable rural development. (author)

Sterilization of fruit flies (Diptera: Tephritidae) with X-rays for sterile insect technique programs; Esterilizacao de moscas-das-frutas (Diptera: Tephritidae) com raios-X para programas de tecnica do inseto esteril

Energy Technology Data Exchange (ETDEWEB)

Mastrangelo, Thiago de Araujo

2009-07-01

Recent fear of acts of terrorism provoked an increase of delays and denials in the shipment of radioisotopes. This truly represented a menace to sterile insect production projects around the world. In order to validate the use of a new kind of low-energy Xray irradiator, a series of radiobiological studies on Ceratitis capitata (tsl-VIENNA 8 strain) (Wied., 1824) (Diptera: Tephritidae) and an Argentinean strain of Anastrepha fraterculus (Wied., 1830) (Diptera: Tephritidae) were carried out, also comparing biological effectiveness between X-rays and traditional {gamma} radiation from {sup 60}Co. Pupae 48- 24 h before adult emergence of C. capitata males and both sexes of A. fraterculus were irradiated with doses ranging from 15 to 120 Gy and 10 to 70 Gy respectively. Doses that induce 50, 90 and 99% of sterility were estimated and the hypothesis of Parallelism for the Probit equations was tested. Doses of 82.7 Gy of X-rays and 128.2 Gy of {gamma} rays (thus, a RBE{approx}1.5) induced 99% sterility on medfly males. The fertility of A. fraterculus fertile females crossed with 41 Gy of X-rays and 62.7 Gy of {gamma} rays decreased in 99% comparing with the control group (RBE{approx}1.5). 99% sterility of A. fraterculus irradiated females was achieved with 60-80 Gy (RBE{approx}0.7). The standard quality control parameters of fecundity, adult emergence, fliers and survival were not significantly affected by the two types of radiation (RBE{approx}1) either for medfly or A. fraterculus (p>0.01), being averages in conformity with the values required by FAO/IAEA/USDA. Only fecundity of irradiated A. fraterculus females was severely reduced with increasing doses and no egg was laid at 70 Gy of both radiations. There were no significant differences between X-rays and {gamma} rays regarding mating indices (RSI for medfly, RII, ISI, MRPI and FRPI for A. fraterculus) (p>0.05), what indicated more random matings for fertile and sterile insects. The results demonstrated that no

Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of Enterobacter sp. in Larval Diet-Based Probiotic Applications.

Directory of Open Access Journals (Sweden)

Antonios A Augustinos

Full Text Available The Mediterranean fruit fly (medfly, Ceratitis capitata, is a pest of worldwide substantial economic importance, as well as a Tephritidae model for sterile insect technique (SIT applications. The latter is partially due to the development and utilization of genetic sexing strains (GSS for this species, such as the Vienna 8 strain, which is currently used in mass rearing facilities worldwide. Improving the performance of such a strain both in mass rearing facilities and in the field could significantly enhance the efficacy of SIT and reduce operational costs. Recent studies have suggested that the manipulation of gut symbionts can have a significant positive effect on the overall fitness of insect strains. We used culture-based approaches to isolate and characterize gut-associated bacterial species of the Vienna 8 strain under mass rearing conditions. We also exploited one of the isolated bacterial species, Enterobacter sp., as dietary supplement (probiotic to the larval diet, and we assessed its effects on fitness parameters under the standard operating procedures used in SIT operational programs. Probiotic application of Enterobacter sp. resulted in improvement of both pupal and adult productivity, as well as reduced rearing duration, particularly for males, without affecting pupal weight, sex ratio, male mating competitiveness, flight ability and longevity under starvation.

Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of Enterobacter sp. in Larval Diet-Based Probiotic Applications

Science.gov (United States)

Papadopoulos, Nikos T.; Abd-Alla, Adly M. M.; Cáceres, Carlos; Bourtzis, Kostas

2015-01-01

The Mediterranean fruit fly (medfly), Ceratitis capitata, is a pest of worldwide substantial economic importance, as well as a Tephritidae model for sterile insect technique (SIT) applications. The latter is partially due to the development and utilization of genetic sexing strains (GSS) for this species, such as the Vienna 8 strain, which is currently used in mass rearing facilities worldwide. Improving the performance of such a strain both in mass rearing facilities and in the field could significantly enhance the efficacy of SIT and reduce operational costs. Recent studies have suggested that the manipulation of gut symbionts can have a significant positive effect on the overall fitness of insect strains. We used culture-based approaches to isolate and characterize gut-associated bacterial species of the Vienna 8 strain under mass rearing conditions. We also exploited one of the isolated bacterial species, Enterobacter sp., as dietary supplement (probiotic) to the larval diet, and we assessed its effects on fitness parameters under the standard operating procedures used in SIT operational programs. Probiotic application of Enterobacter sp. resulted in improvement of both pupal and adult productivity, as well as reduced rearing duration, particularly for males, without affecting pupal weight, sex ratio, male mating competitiveness, flight ability and longevity under starvation. PMID:26325068

Standard Operating Procedures for Preparing and Handling Sterile Male Tsetse flies for Release

International Nuclear Information System (INIS)

Argiles-Herrero, Rafa; Leak, Stephen G.A.

2016-01-01

The purpose of this SOP is to describe the procedures involved in preparing tsetse flies reared in a breeding facility for release in the field for the sterile insect technique (SIT) as a component of Area-Wide Insect pest Management (AW-IPM). Following the procedures which are outlined will help to ensure that the released sterile male tsetse flies are of optimal quality.

Studies on mating competitiveness of sterile oriental fruit fly, Bactrocera dorsalis (Hendel)

International Nuclear Information System (INIS)

Limohpasmanee, W.; Segsarnviriya, S.

1998-01-01

An essential prerequisite for insect control by the sterile insect technique releasing method is mass rearing and sterilizing that do not have adverse effects on longevity and mating behavior of the released males. But many laboratory studies have shown that males irradiated at the completely sterility dose often could not compete with untreated males in mating. This paper studies the effects of gamma radiation at the sterile dose on mating, sexual and sperm competitiveness of the oriental fruit fly, Bactrocera dorsalis (Hendel) under the laboratory condition. It is found that irradiation at the completely sterility dose (90 Gy) had reduced the mating and sperm competition ability of the males. Though the sexual competition was not

Sterility test by radiometric technique

International Nuclear Information System (INIS)

Faruq, Muhammad

1980-01-01

Sterility test of pharmaceuticals can be carried out by the application of pharmacopoeia and radiometric technique. In Indonesia the application of pharmacopoeia technique is carried out through liquid germination for aerobacteria and for fungus and yeast. Radiometric technique is applied to autotrop and heterotrop bacteria. (SMN)

Insect and pest control newsletter. No. 53

International Nuclear Information System (INIS)

1999-07-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted

Insect and pest control newsletter. No. 56

Energy Technology Data Exchange (ETDEWEB)

NONE

2001-01-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted.

Insect and pest control newsletter. No. 55

Energy Technology Data Exchange (ETDEWEB)

NONE

2000-07-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted.

Insect and pest control newsletter. No. 55

International Nuclear Information System (INIS)

2000-07-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted

Insect and pest control newsletter. No. 54

International Nuclear Information System (INIS)

2000-01-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted

Insect and pest control newsletter. No. 56

International Nuclear Information System (INIS)

2001-01-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted

Insect and pest control newsletter. No. 52

International Nuclear Information System (INIS)

1998-12-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted

Insect and pest control newsletter. No. 50

International Nuclear Information System (INIS)

1997-10-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted

Insect and pest control newsletter. No. 51

International Nuclear Information System (INIS)

1998-06-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted

Insect and pest control newsletter. No. 51

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-06-01

This Newsletter announces research coordination meetings, status of existing research coordinated research programmes on the use of nuclear applications such as the sterile insect technique (SIT) in insect and pest control. Training courses as well as new coordinated research programmes in the pipeline are also highlighted.

Estimation of populations and sterility induction in Anastrepha ludens (Diptera: Tephritidae) fruit flies

International Nuclear Information System (INIS)

Flores, Salvador; Montoya, Pablo; Toledo, Jorge; Liedo, Pablo; Enkerlin, Walther

2015-01-01

The relationship between different release densities of sterile flies and fly trap captures, expressed as flies per trap per day, in the monitoring of Anastrepha ludens (Loew) populations was evaluated in mango orchards. The induction of sterility in fertile females was evaluated using different ratios of sterile: fertile males under field cage conditions. A direct relationship between recaptured flies and densities of release sterile flies was found. However, trap efficiency, expressed as percentage of recaptured flies, decreased as the density of released flies increased. Sterility induction was positively correlated to the ratio of sterile: fertile flies. A significant difference in egg fertility among treatments was observed. The trajectory of sterility induction slowed down after a sterile: wild ratio of 30:1, which suggests that this ratio could be appropriate in an sterile insect technique program with A. ludens. Sterility induction was greater when only sterile males were released than when releasing both sterile males and females, but the differences were not significant. Our findings contribute to a better interpretation of fly captures obtained from the field trapping networks, and to an improvement in the efficiency of sterile insect technique against A. ludens fruit flies, through the implementation of more rational sterile fly release densities. (author)

Electron sterilization validation techniques using the controlled depth of sterilization process

International Nuclear Information System (INIS)

Cleghorn, D.A.; Nablo, S.V.

1990-01-01

Many pharmaceutical products, especially parenteral drugs, cannot be sterilized with gamma rays or high energy electrons due to the concomitant product degradation. In view of the well-controlled electron energy spectrum available in modern electron processors, it is practical to deliver sterilizing doses over depths considerably less than those defining the thickness of blister-pack constructions or pharmaceutical containers. Because bremsstrahlung and X-ray production are minimized at these low electron energies and in these low Z materials, very high electron: penetrating X-ray dose ratios are possible for the application of the technique. Thin film dosimetric techniques have been developed utilizing radiochromic film in the 10-60 g/m 2 range for determining the surface dose distribution in occluded surface areas where direct electron illumination is not possible. Procedures for validation of the process using dried spore inoculum on the product as well as in good geometry are employed to determine the process lethality and its dependence on product surface geometry. Applications of the process to labile pharmaceuticals in glass and polystyrene syringes are reviewed. It has been applied to the sterilization of commercial sterile products since 1987, and the advantages and the natural limitations of the technique are discussed. (author)

'More than two': integrating biological control and sterile insects, from factory to field, and the possibility of its implementation in Argentina

International Nuclear Information System (INIS)

Cladera, Jorge L.; Viscarret, Mariana M.; Carabajal Paladino, Leonela Z.; Pietrek, Alejandro; Soria, M. Alejandra; Ovruski, Sergio M.

2006-01-01

No single control measure is able to provide full control of a pest. Integration of techniques like the sterile insect (SIT) with biological control practices (BC) should be intensively sough for. This possibility is revised here in connection with the fruit fly pest problems in Argentina. Theoretical reasons as well as practical conveniences for this integration are reviewed in this paper, the intention of which is to promote a discussion on how to approach the experimental study of the SIT+CB integration problem, i.e. how to measure the effects of each separate control measure as well as that of both acting together, in a repeatable manner. Arguments are advanced in favor of the joint production and releases of sterile fruit flies and parasitoids. (author)

Factors affecting the efficiency of the sterile insect release method for tsetse

International Nuclear Information System (INIS)

Curtis, C.F.

1980-01-01

Data are reviewed on the levels of sterility, survival and competitiveness of Glossina males after irradiation with various gamma-ray doses delivered in air or nitrogen. A simple population model helps in the choice of the optimum dose. Field studies of mating competitiveness require a measure of the ratio of sterile to fertile males and of sterile to fertile matings, and both ratio estimates will be subject to sampling error. Data on multiple mating of female Glossina are reviewed. There is some degree of precedence for sperm from the first mating but sterile sperm are fully competitive for fertilization and, following the early death of an embryo, the timing of the next ovulation is only advanced to a slight extent. Thus in an isolated population the occurrence or non-occurrence of female polygamy would be of almost no consequence. Where mated females were immigrating into the release area female polygamy would be advantageous. The low recovery potential of tsetse populations is the main reason for thinking them suitable for control by the introduction of sterility. In trying to measure this recovery potential it is important to distinguish it from natural seasonal increase. If natural population fluctuations are studied to detect density dependent effects it is important not to confuse cause with effect. Non-isolation of the 'target' population is almost certainly the most serious obstacle to practical application of the sterile insect release method, and a steady 'rolling forward' of the release area may be a possible solution. (author)

Radiations: tool for insect pest management

International Nuclear Information System (INIS)

Swami, Kailash Kumar; Kiradoo, M.M.; Srivastava, Meera

2012-01-01

The discovery that X-rays or gamma radiation could cause sufficient genetic damage to insect reproductive systems to induce sterility resulted from work conducted by H.J. Muller starting in the 1920s. The sterilizing effect of radiation was noted by scientists of the US Department of Agriculture who had been seeking a method to sterilize insects for many years. These scientists had theorized that if large numbers of the target insect species were reared, sterilized, and released into the field, the sterile insects would mate with the wild insects. These mating would result in no offspring and thus a decline in the population would be obtained. They calculated that if sufficient numbers of sterile insects were released, reproductive rate for the wild population would rapidly decline and reach zero. In simple language, birth control of insects. Radiation sterilization was the answer. In a SIT operation, radiation is used to sexually sterilize insects. Since the SIT is species specific, the selection the insect pest or group of pests on which to work is of primary importance. The Joint Division of the IAEA Food and Agriculture Organization (FAO) has been involved in the use of isotopes and radiation in insect control since 1964. Isotopes are used as tags or markers, for instance, of chemical molecules, insects, or plants. For example, with these tags one can follow the fate of insecticides within insects and the environment; the incorporation of nutrients into the insect; and the movements of insects under field conditions. They also can plants on which insects feed so that the quantity of consumed food can be measured and directly correlated with plant resistance. They can be used as well to follow parasites and predators of insects - for example, their movements, numbers, and ability to help control insect pests. Radiations therefore have come as a novel tool to combat insect pest problem and in future could be very helpful in various other ways, of be it be cost

'More than two': integrating biological control and sterile insects, from factory to field, and the possibility of its implementation in Argentina

Energy Technology Data Exchange (ETDEWEB)

Cladera, Jorge L.; Viscarret, Mariana M.; Carabajal Paladino, Leonela Z.; Pietrek, Alejandro [Instituto de Tecnologia Agropecuaria (INTA), Castelar (Argentina). Inst. de Genetica; Soria, M. Alejandra; Ovruski, Sergio M. [Consejo Nacional de Investigaciones Cientificas y Tecnicas (PROIMI/CONICET), Tucuman (Argentina). Planta Piloto de Procesos Industrials Microbiolo

2006-07-01

No single control measure is able to provide full control of a pest. Integration of techniques like the sterile insect (SIT) with biological control practices (BC) should be intensively sough for. This possibility is revised here in connection with the fruit fly pest problems in Argentina. Theoretical reasons as well as practical conveniences for this integration are reviewed in this paper, the intention of which is to promote a discussion on how to approach the experimental study of the SIT+CB integration problem, i.e. how to measure the effects of each separate control measure as well as that of both acting together, in a repeatable manner. Arguments are advanced in favor of the joint production and releases of sterile fruit flies and parasitoids. (author)

The sterile-male technique for control of the Mediterranean fruit fly, Ceratitis capitata Wied., in the Mediterranean basin

International Nuclear Information System (INIS)

Serghiou, C.

1975-01-01

Certain problems caused by the use of insecticides in the management of agricultural pests, such as environmental pollution, insecticide resistance and disturbance of biological balance, has led to the development of selective pest control methods. A prominent place among these for the control of the Mediterranean fruit fly has been attained by the species specific sterile-insect technique (SIT). This study reviews the status of field programmes in countries of the Mediterranean basin, and of related mass rearing, irradiation and field release methodology. The SIT has been successfully tested in Spain, Italy, Israel and Cyprus. In all these cases, however, tests were conducted in small semi-isolated areas where at best a high degree of suppression but not eradication of the fly could be obtained since immigration of gravid females was always possible. The SIT programme in Cyprus and data on medfly ecology in the island is here reviewed in more detail. A proposal is make for the eradication of medfly from Cyprus by the use of an integration of methods, namely bait spraying, cultural practices and sterile-insect releases. (author)

Sterilization experiment of crocidolomia binotalis zeller by gamma ray irradiation and its control application

International Nuclear Information System (INIS)

Sutrisno, Singgih

1986-01-01

Experiments have been conducted to study C. binotalis control initiated with the rearing method and ended with the release of sterile irradiated insects. Rearing of cabbage pest using modified artificial diet of Pieris rapae gave better result compared to that with natural diet. The larval viability was 58% and 25.9% from the artificial and the natural diet respectively. Gamma ray doses of 0, 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4 and 0.45 kGy were introduced to six-day-old sexed pupae in nitrogen and air atmosphere. A 100% sterility on males was obtained at 0.45 kGy in both air and nitrogen atmosphere. 100% sterility on females was found at 0.25 kGy in nitrogen and 0.2 kGy in air atmosphere. The effect of dose on the percentage of sterile and mating competitiveness were not significantly different (P ≥0.05). A significant correlation (r=0.8774) was found between the percentage of fertility and the mating competitiveness of irradiated insects. The lower the fertility of the insects the less mating competitiveness they have. 0.4 kGy was considered to be adequate for sterilization of C. binotalis. It produced a high percentage of sterility besides moderate mating competitiveness, 84.52% and 0.57% respectively. 0.4 kGy seemed to give 3 days shorter longevity of moth than the normal one. The number of F 1 larval population was not significantly different if the released insects were either females or males or mixed sexes. The ratio of 9:1 of the sterile and normal insects under laboratory, field-cage, and in the field indicated that the sterile insect technique has the potential to suppress F 1 population by about 50% due to the mating competitiveness of the radiosterilized insect still maintained at the level of 0.57. (author). 17 refs

Comparison of Sterile and Clean Dressing Techniques in Post ...

African Journals Online (AJOL)

Comparison of Sterile and Clean Dressing Techniques in Post-operative Surgical Wound Infection in a Chinese Healthcare Facility. ... of sterile and clean dressing techniques on wound management in a Chinese hospital, and to compare their impact on wound healing and the cost of the dressing materials with respect to ...

Automation for tsetse mass rearing for use in sterile insect technique programmes. Final report of a co-ordinated research project 1995-2001

International Nuclear Information System (INIS)

2003-05-01

The rearing of tsetse flies for the sterile insect technique has been a laborious procedure in the past. The purpose of this co-ordinated research project (CRP) 'Automation for tsetse mass rearing for use in sterile insect technique programmes' was to develop appropriate semiautomated procedures to simplify the rearing, reduce the cost and standardize the product. Two main objectives were accomplished. The first was to simplify the handling of adults at emergence. This was achieved by allowing the adults to emerge directly into the production cages. Selection of the appropriate environmental conditions and timing allowed the manipulation of the emergence pattern to achieve the desired ratio of four females to one male with minimal un-emerged females remaining mixed with the male pupae. Tests demonstrated that putting the sexes together at emergence, leaving the males in the production cages, and using a ratio of 4:1 (3:1 for a few species) did not adversely affect pupal production. This has resulted in a standardized system for the self stocking of production cages. The second was to reduce the labour involved in feeding the flies. Three distinct systems were developed and tested in sequence. The first tsetse production unit (TPU 1) was a fully automated system, but the fly survival and fecundity were unacceptably low. From this a simpler TPU 2 was developed and tested, where 63 large cages were held on a frame that could be moved as a single unit to the feeding location. TPU 2 was tested in various locations, and found to satisfy the basic requirements, and the adoption of Plexiglas pupal collection slopes resolved much of the problem due to light distribution. However the cage holding frame was heavy and difficult to position on the feeding frame and the movement disturbed the flies. TPU 2 was superseded by TPU 3, in which the cages remain stationary at all times, and the blood is brought to the flies. The blood feeding system is mounted on rails to make it

Mediterranean fruit fly female attractant studies in support of the sterile insect technique: trapping experiments conducted on the island of Chios, Greece

International Nuclear Information System (INIS)

Katsoyannos, B.I.; Papadopoulos, N.T.; Kouloussis, N.A.

1999-01-01

This paper contains information on a four-year research programme co-ordinated by the International Atomic Energy Agency. The main objective of the programme was to develop a trapping system for females of the Mediterranean fruit fly (medfly), Ceratitis capitata (Diptera: Tephritidae), for practical use in Sterile Insect Technique (SIT) programs and to design and evaluate a trap to obtain eggs from wild female medflies in order to estimate sterility induction in the field population. The experiments were conducted from July to September 1994-1997 on the island of Chios, Greece, in citrus orchards with low to medium medfly populations. Different trap types and several trap treatments consisting of sex and food based attractants were tested, following a standard coordinated experimental protocol. The most extensively tested were three food based 'female' attractants (FA-3), namely ammonium acetate (AA), 1,4 diaminobutane (putrescine) and trimethylamine, all formulated in dispensers lasting one month. These attractants were evaluated in combinations of two (AA + putrescine, termed FA-2) or three (FA-3) dispensers in various traps, including dry (provided with DDVP) or wet (provided with water and 0.01% surfactant) plastic International Pheromone's McPhail traps (IPMT). Among the various traps and treatments tested, the most effective for medfly capture was the wet IPMT, baited with FA-3 attractants. This treatment captured predominantly females and was relatively selective for medflies. In dry IPMT traps, the FA-3 were as effective as the standard 300 ml aqueous solution of 9% of the protein NuLure and 3% borax, but much more medfly selective. Dry IPMT traps were also more selective than wet ones. FA-3 baited wet Tephri traps (a Spanish modification of the McPhail trap), performed somewhat poorer than IPMT traps. Other dry trap types tested were not effective. Additional experiments showed that certain insecticide formulations used in dry traps may have a repellent

Application of radiation and radioisotopes for the management of insect pests of economic importance

International Nuclear Information System (INIS)

Dongre, T.K.

2001-01-01

This article gives brief account of radiation and radioisotope applications in the field of insect pest management. Radiation has a direct application in controlling insect pests, through sterile insect techniques (SIT) and radiation disinfestations of food grains, whereas radioisotopes can be used in basic as well as applied studies in the field of insect physiology, ecology and metabolism. The successful implementation of SIT against New world screwworm fly and different fruit fly species has clearly demonstrated the usefulness of radiation in agriculture. Over the past 35 years, the joint FAO/IAEA committee has played a critical role in supporting member states in the development and application of SIT for the management of various economically important insect pests. BARC has developed SIT for the management of red palm weevil, Rhynchophorus ferrugineus Oliv, the most serious pest of coconut in India and date palms in Arabian countries. Now, through thematic BRNS project this technique is being evaluated under field conditions in collaboration with three Indian agricultural universities. Present status and future prospects of sterile insect technique for the area wide control of different insect species will be discussed in detail. (author)

Development of mass production, gamma sterilization and release of the codling moth, Laspeyresia pomonella L. Part of a coordinated programme on the use of the sterile male technique for control of lepidopterous insects attacking fruit and forest trees

International Nuclear Information System (INIS)

Suski, Z.W.

1979-03-01

Research on codling moths was conducted from Spring 1973 to Autumn 1978 and included rearing of larvae on thinning apples and artificial diet ecology, radiation sterilization and the effect of field releases of sterile moths in suppressing the wild population. Field releases of irradiated with 30 Krad unsexed insects were conducted in two consecutive seasons, namely in 1977 and 1978 and aimed at a sterile to wild ratio of 97:1 and 233:1 respectively. The experimentally obtained ratio, however, based on catches in traps baited with sex attractant was 24:1 and 79:1 respectively. Examination of fruit infestation at harvest revealed an increase in infestation by 9% in the 1977 sterile release programme and by 56% in the 1978 programme. Likely causes of the failure of the SIT programme were the immigration of fertile females from the surrounding area and inadequate mating competitiveness of the released moths due to somatic damage caused by the irradiation and laboratory adaptation. The sharp increase of fruit infestation in 1978 was very likely the result of a six fold increase in yield which provided a better chance of survival for hatching larvae

Management of insect pests: Nuclear and related molecular and genetic techniques

International Nuclear Information System (INIS)

1993-01-01

The conference was organized in eight sessions: opening, genetic engineering and molecular biology, genetics, operational programmes, F 1 sterility and insect behaviour, biocontrol, research and development on the tsetse fly, and quarantine. The 64 individual contributions have been indexed separately for INIS. Refs, figs and tabs

Insect pest control newsletter. No. 65

International Nuclear Information System (INIS)

2005-07-01

The concept of Area-wide Integrated Pest Management (AW-IPM) is defined as IPM applied against an entire pest population within a delimited geographic area. Area-wide intervention strategies require more planning and ecological understanding, longer-term commitment, a minimum infrastructure and a coordinated implementation by farmers and all other stakeholders. The spatial distribution of the pest population has to be considered not only in surrounding cultivated areas, but also in non-cultivated areas. It also involves considering the temporal distribution of the pest to determine the periods when the pest is most susceptible to preventive, rather than remedial, interventions. In 1998 FAO and the Agency sponsored the First International Conference on 'Area-Wide Control of Insect Pests, Integrating the Sterile Insect and Related Nuclear and other Techniques' in Penang, Malaysia. This Conference greatly increased the interest and awareness concerning the AW-IPM approach to insect pest control. Since then, many new technical innovations have been introduced; a better regulatory framework is being developed to encourage the involvement of the private sector, and more FAO and Agency Member States are integrating insect pest control methods on an areawide basis. Over the past months we have been heavily involved in preparing for the Second FAO/IAEA International Conference on 'Area-Wide Control of Insect Pests: Integrating the Sterile Insect and Related Nuclear and Other Techniques', which was held from 9-13 May in Vienna. The response and interest of scientists and governments, as well as the private sector and sponsors were once more very encouraging. The conference took place with the participation of over 300 delegates from 86 countries, nine international organization, and eight exhibitors. It covered the area-wide approach again in a very broad sense, including the development and integration of many non-SIT technologies, as well as genetic research on cytoplasmic

Insect pest control newsletter. No. 65

Energy Technology Data Exchange (ETDEWEB)

NONE

2005-07-01

The concept of Area-wide Integrated Pest Management (AW-IPM) is defined as IPM applied against an entire pest population within a delimited geographic area. Area-wide intervention strategies require more planning and ecological understanding, longer-term commitment, a minimum infrastructure and a coordinated implementation by farmers and all other stakeholders. The spatial distribution of the pest population has to be considered not only in surrounding cultivated areas, but also in non-cultivated areas. It also involves considering the temporal distribution of the pest to determine the periods when the pest is most susceptible to preventive, rather than remedial, interventions. In 1998 FAO and the Agency sponsored the First International Conference on 'Area-Wide Control of Insect Pests, Integrating the Sterile Insect and Related Nuclear and other Techniques' in Penang, Malaysia. This Conference greatly increased the interest and awareness concerning the AW-IPM approach to insect pest control. Since then, many new technical innovations have been introduced; a better regulatory framework is being developed to encourage the involvement of the private sector, and more FAO and Agency Member States are integrating insect pest control methods on an areawide basis. Over the past months we have been heavily involved in preparing for the Second FAO/IAEA International Conference on 'Area-Wide Control of Insect Pests: Integrating the Sterile Insect and Related Nuclear and Other Techniques', which was held from 9-13 May in Vienna. The response and interest of scientists and governments, as well as the private sector and sponsors were once more very encouraging. The conference took place with the participation of over 300 delegates from 86 countries, nine international organization, and eight exhibitors. It covered the area-wide approach again in a very broad sense, including the development and integration of many non-SIT technologies, as well as genetic research on cytoplasmic

Dipping of Eggs, Use of Low Temperature and Aeration to Improve Fruit Fly Bactrocera Carambolae (Drew and Hancock) Mass Rearing in Sterile Insect Technique Programme

International Nuclear Information System (INIS)

Nasution, Indah Arastuti; Achmad Nasroh Kuswadi

2004-01-01

In the Sterile Insect Technique (SIT) programme a large number of homogenous age of pupae should be produced to be sterilized and released into the field. A methodology to preserve eggs which at the same time delay the egg hatch is needed in the production of a large number of pupae with homogenous age. The study on method of preserving fruit fly eggs the effect of dipping, low temperature, on the viability of eggs, the quality of larvae hatched from the eggs, and the quality of pupae produced were observed. Viability of eggs were observed as percent of egg hatch, quality of larvae as number of pupae produced from 0.5 ml of eggs when reared on artificial diet, and quality of pupae as percent of the flies number emerged and flew from the pupae. In room temperature (26 o C) dipping did not preserve fruit fly eggs and aeration did not improve the preservation. Although after one day dipping the viability of eggs only decreased from 90 % (control) to 80.5 %, (without aeration) and 81.5 % (with aeration) it decreased the number of pupae been produced from 1447 pupae (control) to 382 pupae (without aeration) and 616 pupae (with aeration). Low temperature successfully preserved the egg up to 24 hours. After 24 hour dipping in low temperature (16 o C) the viability of eggs were decreased up to 84.75 % (without aeration) and able to produce 1104 pupae. Aeration during dipping in low temperature did not improve the preservation. (author)

The use of different concentrations of ginger root oil to improve the mating propensity of the Medfly sterile males

International Nuclear Information System (INIS)

Fadhl, Salma; Msaad Guerfali, M.; Kahlani, A.; Hamden, Haithem; Chevrier, C.

2008-01-01

The sterile insect technique in an efficient technique used to control fruit flies such as Medfly Ceratitis Capitata (Wiedemann) (Diptera : Tephritidae). Unfortunately, this technique involves mass breeding of huge quantities of target insects in the factory and sterilizing the males by exposing them to low doses of radiation. this procedure reduces the mating propensity of the sterile males which are the active agent of the technique. It was reported that the exposition of the sterile males to ginger root oil (Zingiber official Roscoe (Zingiberaceae)) improves the mating propensity of the sterile males in comparison with the non exposed ones. In this study, we exposed irradiated males at two different doses (100 and 110 Gy) to different concentrations of the ginger oil (20μl; 50μl; 80μl; 0.25 ml;0.5 ml; 1 ml) the third day after emergence. This study shows that the irradiated males at 100 Gy are more competitive in comparison with those irradiated at 110 Gy with a mating propensity percentage respectively of 60 and 35 %. regarding the exposition dose of the ginger oil, 1 ml gave the best mating propensity ( 42%) in comparison with the other concentrations

Insect Pest Control Newsletter, No. 81, July 2013

International Nuclear Information System (INIS)

2013-07-01

In response to requests from our readers, this introduction is mainly dedicated to the ongoing efforts to develop alternatives for insect reproductive sterilization and blood sterilization for their use in insect pest control programmes with a sterile insect technique (SIT) component. Radioisotope irradiators that are loaded with either cobalt-60 or caesium-137 producing gamma rays have been routinely used for many decades and have proven to be extremely reliable and safe for these purposes in successful area-wide insect eradication or suppression programmes. These include industrial panoramic-type irradiators in larger programmes, all the way to smaller self-contained irradiators. Nevertheless, the transboundary shipment of self-contained gamma irradiators or radioactive material has become logistically more complex due to security issues. This situation was exacerbated when the production of the Gamma Cell 220 (GC220), the source most commonly used for irradiating insects for sterilization purposes, was discontinued. These events may have created the impression that the use of gamma radiation has become a less viable option, unattainable for insect pest control programmes that want to integrate the SIT. Nevertheless, some of the biggest SIT operational programmes have in recent years been equipped with new self-contained cobalt-60 sources, including the SIT programme against the pink bollworm in Phoenix, Arizona; El Pino Mediterranean fruit fly facility in Guatemala; and the screwworm programme in Panama. Thus these larger and more expensive irradiators, together with panoramic units (that are also costlier than self-contained gamma irradiators) have remained over the years a valid option, especially for larger operational programmes. In addition, the reloading of smaller units with new cobalt or the purchase of refurbished used self-contained irradiators remain viable alternatives

Insect Pest Control Newsletter, No. 81, July 2013

Energy Technology Data Exchange (ETDEWEB)

NONE

2013-07-15

In response to requests from our readers, this introduction is mainly dedicated to the ongoing efforts to develop alternatives for insect reproductive sterilization and blood sterilization for their use in insect pest control programmes with a sterile insect technique (SIT) component. Radioisotope irradiators that are loaded with either cobalt-60 or caesium-137 producing gamma rays have been routinely used for many decades and have proven to be extremely reliable and safe for these purposes in successful area-wide insect eradication or suppression programmes. These include industrial panoramic-type irradiators in larger programmes, all the way to smaller self-contained irradiators. Nevertheless, the transboundary shipment of self-contained gamma irradiators or radioactive material has become logistically more complex due to security issues. This situation was exacerbated when the production of the Gamma Cell 220 (GC220), the source most commonly used for irradiating insects for sterilization purposes, was discontinued. These events may have created the impression that the use of gamma radiation has become a less viable option, unattainable for insect pest control programmes that want to integrate the SIT. Nevertheless, some of the biggest SIT operational programmes have in recent years been equipped with new self-contained cobalt-60 sources, including the SIT programme against the pink bollworm in Phoenix, Arizona; El Pino Mediterranean fruit fly facility in Guatemala; and the screwworm programme in Panama. Thus these larger and more expensive irradiators, together with panoramic units (that are also costlier than self-contained gamma irradiators) have remained over the years a valid option, especially for larger operational programmes. In addition, the reloading of smaller units with new cobalt or the purchase of refurbished used self-contained irradiators remain viable alternatives.

Exploiting novel sterilization techniques for porous polyurethane scaffolds.

Science.gov (United States)

Bertoldi, Serena; Farè, Silvia; Haugen, Håvard Jostein; Tanzi, Maria Cristina

2015-05-01

Porous polyurethane (PU) structures raise increasing interest as scaffolds in tissue engineering applications. Understanding the effects of sterilization on their properties is mandatory to assess their potential use in the clinical practice. The aim of this work is the evaluation of the effects of two innovative sterilization techniques (i.e. plasma, Sterrad(®) system, and ozone) on the morphological, chemico-physical and mechanical properties of a PU foam synthesized by gas foaming, using water as expanding agent. In addition, possible toxic effects of the sterilization were evaluated by in vitro cytotoxicity tests. Plasma sterilization did not affect the morphological and mechanical properties of the PU foam, but caused at some extent degradative phenomena, as detected by infrared spectroscopy. Ozone sterilization had a major effect on foam morphology, causing the formation of new small pores, and stronger degradation and oxidation on the structure of the material. These modifications affected the mechanical properties of the sterilized PU foam too. Even though, no cytotoxic effects were observed after both plasma and ozone sterilization, as confirmed by the good values of cell viability assessed by Alamar Blue assay. The results here obtained can help in understanding the effects of sterilization procedures on porous polymeric scaffolds, and how the scaffold morphology, in particular porosity, can influence the effects of sterilization, and viceversa.

Dispersal and survival of sterile male (TSL strain) Mediterranean fruit flies

International Nuclear Information System (INIS)

Barbosa, S.; Mexia, A.; Pereira, R.

2000-01-01

The Mediterranean fruit fly, Ceratitis capitata (Weid.), is a major pest of fruit in the Portuguese Autonomous Region of Madeira (Vieira 1952). The Medfly attacks more than 40 species of fruit (Vieira 1952, Pereira et al. 1996). It was reported as a pest primarily below 400 m but occurred up to 700 m on the south coast. Madeira (32 deg. N, 17 deg. W) is located 980 km west-southeast of mainland Portugal. Its two principal islands (Porto Santo (50 km 2 ) and Madeira (740 km 2 )) are populated by 255,000 people. Fruit and vegetable production is widespread on Madeira island but not on Porto Santo island because of poor soil and limited rainfall. The climate of Madeira is variable, depending upon altitude and location (northern/southern). On the whole, the climate is moderated by the effects of the surrounding sea. The sterile insect technique (SIT) is a genetic method of insect control. Large numbers (frequently more than 50 million/week) of the target insect are reared in specially designed factories (Pereira et al., in press). These insects are sterilised with gamma radiation and released by aircraft into the target area. Mating between the factory reared sterile males and fertile wild females produces no progeny. Thus, if sufficient sterile males are introduced into the target area on a continuous basis there is a very high probability that fertile wild females will mate with sterile males (Hendrichs et al. 1995). Under these conditions, the birth rate of the target species is greatly reduced and will rapidly reach zero if no fertile insects are brought into the target area. Little work has been done with all male releases because it is only very recently that all male strains have been mass produced. It has been postulated that releasing only sterile male Medflies could result in better distribution and perhaps increased longevity of the released individuals. To evaluate this hypothesis, tests of longevity and dispersion of sterile males were conducted. Ground

Sterility and meeting competitiveness of medfly, Ceratitis Capitata (Wiedemann)

International Nuclear Information System (INIS)

Taein-Tun; Molly-Maung-Maung

2001-01-01

The basic methodology in the determination of sterilising dosage in male med flies and mating competitiveness with the normal males was carried out in the laboratory. Application of the Sterile Insect Technique (S.I.T.) by three irradiation dosages on Seib-6096 pupae results in sterility when dosage increased. A lower mating competitiveness was observed with the increase in sterility value. This value was determined from the corrected egg hatch percent. The resulting data showed that irradiation dosage of γ 10.0 Krad gave a good advantage to suppress the population in the next generation. The method suggested a good application in the control and eradication of fruit flies. (author)

Sterility and Sexual Competitiveness of Tapachula-7 Anastrepha ludens Males Irradiated at Different Doses.

Science.gov (United States)

Orozco-Dávila, Dina; Adriano-Anaya, Maria de Lourdes; Quintero-Fong, Luis; Salvador-Figueroa, Miguel

2015-01-01

A genetic sexing strain of Anastrepha ludens (Loew), Tapachula-7, was developed by the Mexican Program Against Fruit Flies to produce and release only males in programs where the sterile insect technique (SIT) is applied. Currently, breeding are found at a massive scale, and it is necessary to determine the optimum irradiation dose that releases sterile males with minimum damage to their sexual competitiveness. Under laboratory and field conditions, we evaluated the effects of gamma irradiation at doses of 0, 20, 40, 60 and 80 Gy on the sexual competitiveness of males, the induction of sterility in wild females and offspring survivorship. The results of the study indicate that irradiation doses have a significant effect on the sexual behavior of males. A reduction of mating capacity was inversely proportional to the irradiation dose of males. It is estimated that a dose of 60 Gy can induce more than 99% sterility in wild females. In all treatments, the degree of offspring fertility was correlated with the irradiation dose of the parents. In conclusion, the results of the study indicate that a dose of 60 Gy can be applied in sterile insect technique release programs. The application of this dose in the new genetic sexing strain of A. ludens is discussed.

Sterile Males of Ceratitis Capitata (Diptera: Tephritidae) as Disseminators of Beauveria Bassiana Conidia for IPM Strategies

Energy Technology Data Exchange (ETDEWEB)

Flores, Salvador; Campos, Sergio; Montoya, Pablo [Programa Moscafrut SAGARPA-IICA, Camino a los Cacahotales S/N, Metapa de Dominguez, Chiapas 30860 (Mexico); Villasenor, Antonio; Valle, Alvaro; Enkerlin, Walther [Codireccion Mexico, Programa Regional Moscamed Mexico- Guatemala-Estados Unidos (Guatemala); Toledo, Jorge; Liedo, Pablo [Departamento Agricultura Sociedad y Ambiente, El Colegio de la Frontera Sur. Carretera Antiguo Aeropuerto, Tapachula, Chiapas 30700 (Mexico)

2014-01-15

Full text: Sterile Mediterranean fruit fly, Ceratitis capitata (Wied.), males were evaluated as vectors to spread Beauveria bassiana (Bals) conidia to wild C. capitata populations under field conditions. The inoculated sterile males were released by air, using the chilled adult technique over 7000 ha of coffee growing in Chimaltenango, Guatemala, Central America. The impact of releases was determined using dry traps baited with a food attractant. The effects of these releases on Apis mellifera, Linnaeus (honey bee), Hypothenemus hampei, Ferrari (coffee berry borer) and the parasitic mite Varroa destructor (Oudeman) were also evaluated. Inoculated sterile males were able to transmit fungal spores to 44% of the wild C. capitata flies captured in traps, which likely were infected through intra- and intersexual interactions during leks, mating or mating attempts. There was no transmission of the fungal spores to non- target insect species such as coffee berry borer, honey bees or varroa. We conclude that sterile males of Mediterranean fruit fly inoculated with B. bassiana can act as effective vectors of conidia to wild populations, constituting a safe, environmentally friendly and selective alternative for suppressing the medfly under a Sterile Insect Technique-based IPM approach. (author)

Efficacy of gamma sterilization technique for biofertilizer carrier production

International Nuclear Information System (INIS)

Rallos, Roland V.; Rivera, Faye G.; Anarna, Julie A.; Rojales, Jacqueline S.

2013-01-01

Th use of gamma irradiation as sterilization technique for biofertilizer carrier is very efficient and practical especially in large scale production. The results of this study on the efficacy of gamma sterilization justify research on the effects of established doses of gamma irradiation on the physico-chemical properties of the clay-charcoal mixture

Study of the compatibility and competitiveness of sterile males in the framework of the struggle by the sterile insect technique against the Ceratitis

International Nuclear Information System (INIS)

Azzabi, Jihene

2007-01-01

We evaluated under semi-natural field-cage conditions sexual compatibility and competitivity of laboratory strain (Genetic Sexing Strain GSS) ''Vienna 8''. The GSS strain is produced under mass (rearing conditions at the facility of production of sterile males at Sidi Thabat. Wild flies were obtained from infested Sommer oranges. Twenty-five wild males, 25 wild females and 25 GSS were released into cage and mating pairs collected. The determination of indices such as the Isolation Index (ISI), the Male Relative Index (MRPI), the Stalker Index (I) and the Relative Sterile Index (RSI) shows that there is no compatibility and competitiveness between the two strains with a tendency to behaviour of sexual isolation. Sterile male tests dissection irradiated at the dose of (0 Gy, 110 Gy, 120 Gy, 130 Gy and 145 Gy) and correspondent female spermatic show the important damage of the irradiation on the cells but decrease the quality of the sterile male (mating duration, sperm transfer.). (Author)

Nuclear energy against insect pests

Energy Technology Data Exchange (ETDEWEB)

NONE

1963-07-15

The paper presents the main topics discussed at the scientific symposium on the Use and Application of Radioisotopes and Radiation in the Control of Plant and Animal Insect Pests, held in Athens last April, jointly organized by IAEA and FAO with the co-operation of the Greek Government. The sterile male technique is discussed in details and some results from the applications are given

The sterile-male-release technique in Great Lakes sea lamprey management

Science.gov (United States)

Twohey, Michael B.; Heinrich, John W.; Seelye, James G.; Fredricks, Kim T.; Bergstedt, Roger A.; Kaye, Cheryl A.; Scholefield, Ron J.; McDonald, Rodney B.; Christie, Gavin C.

2003-01-01

The implementation of a sterile-male-release technique from 1991 through 1999 and evaluation of its effectiveness in the Great Lakes sea lamprey (Petromyzon marinus) management program is reviewed. Male sea lampreys were injected with the chemosterilant bisazir (P,P-bis(1-aziridinyl)-N-methylphosphinothioic amide) using a robotic device. Quality assurance testing indicated the device delivered a consistent and effective dose of bisazir. Viability of embryos in an untreated control group was 64% compared to 1% in a treatment group. A task force developed nine hypotheses to guide implementation and evaluation of the technique. An annual average of 26,000 male sea lampreys was harvested from as many as 17 Great Lakes tributaries for use in the technique. An annual average of 16,100 sterilized males was released into 33 tributaries of Lake Superior to achieve a theoretical 59% reduction in larval production during 1991 to 1996. The average number of sterile males released in the St. Marys River increased from 4,000 during 1991 to 1996 to 20,100 during 1997 to 1999. The theoretical reduc-stertion in reproduction when combined with trapping was 57% during 1991 to 1996 and 86% during 1997 to 1999. Evaluation studies demonstrated that sterilized males were competitive and reduced production of larvae in streams. Field studies and simulation models suggest reductions in reproduction will result in fewer recruits, but there is risk of periodic high recruitment events independent of sterile-male release. Strategies to reduce reproduction will be most reliable when low densities of reproducing females are achieved. Expansion of the technique is limited by access to additional males for sterilization. Sterile-male release and other alternative controls are important in delivering integrated pest management and in reducing reliance on pesticides.

Climatic factors and populational dynamics of Mahanarva posticata (Stal-1855) (Homoptera-Cercopidae) as a contribution to a possible regional implantation of the sterile male technique

International Nuclear Information System (INIS)

Oliveira, M.L. de

1981-01-01

A field study on the population dynamics of M. posticata (STAL, 1855) (Homoptera-Cercopidae) was carried out from 1971 to 1978 under the ecological conditions prevailing in the Litoral-Mata-Sul Zone of Pernambuco, Brazil. The results indicated that maximal infestation of the sugar cane crops by the insect generally occured in June and in July, and it was dependent on rainfall, temperature and relative humidity. The population density of adults was decreased markedly during the months of January, February and March. Technical and economical viability of the insect control by the male-sterilization-techniques through radiation was also demonstrated. The technique is to be used isolated or with other conventional procedures in the combat of froghopper infestations in the sugar cane growing in Pernambuco, Brazil. (Author) [pt

Evaluation of the efficiency of various medfly female trapping combinations in Costa Rica in support of the sterile insect technique

International Nuclear Information System (INIS)

Camacho, H.

1999-01-01

This report contains information from a four-year research programme co-ordinated by the International Atomic Energy Agency. The objective of the programme was to develop a trapping system for females of the Mediterranean fruit fly (medfly), Ceratitis capitata (Diptera: Tephritidae), for practical use in Sterile Insect Technique (SIT) programs and to design and evaluate a trap to obtain eggs from wild female medflies in order to estimate sterility induction in the field population. The study was carried out at two different Agricultural Research Stations of the University of Costa Rica, the Fabio Baudrit Agricultural Research Station (FBS) and Laguna de Fraijanes Agricultural Research Station (LFS), and in a privately-owned coffee and orange plantation in Grecia canton (Coope-Victoria Farm). Female medfly attractants tested were three food based 'female' attractants (FA-3), namely ammonium acetate (AA), 1,4 diaminobutane (putrescine) and trimethylamine, all formulated to last at least one month. These attractants were evaluated either in combinations of two (AA + putrescine, termed FA-2) or all three (termed FA-3). The attractants were tested in various traps including the plastic International Pheromone's McPhail traps (IPMT) and Tephri traps, a Spanish trap similar to the IPMT. Traps were used either as a dry trap (provided with DDVP) or a wet trap (provided with water and 0.01% surfactant). Jackson traps with Trimedlure (JT,TML), a routinely used male medfly trapping system, was also used. Trapping experiments conducted in the citrus plantation of the FBS resulted in the following fly/trap/day indices (F/T/D): 7.18 with JT,TML; 4.62 with open bottom dry traps (OBDT) baited with FA-3; 4.18 with OBDT (PVC) baited with FA-3; 7.73 with IPMT baited with FA-3, wet; 8.245 with IPMT baited with FA-3, dry; 5.27 with IPMT baited with NuLure; 4.79 with Tephri baited with FA-3, wet; and 5.42 with Tephri, FA-3, dry. The F/T/D indices at the Coope-Victoria Farm, in coffee

Evaluation of the efficiency of various medfly female trapping combinations in Costa Rica in support of the sterile insect technique

Energy Technology Data Exchange (ETDEWEB)

Camacho, H [Laboratorio de Investigacion en Mosca del Mediterraneo, Escuela de Biologia, Universidad de Costa Rica, San Jose (Costa Rica)

1999-07-01

This report contains information from a four-year research programme co-ordinated by the International Atomic Energy Agency. The objective of the programme was to develop a trapping system for females of the Mediterranean fruit fly (medfly), Ceratitis capitata (Diptera: Tephritidae), for practical use in Sterile Insect Technique (SIT) programs and to design and evaluate a trap to obtain eggs from wild female medflies in order to estimate sterility induction in the field population. The study was carried out at two different Agricultural Research Stations of the University of Costa Rica, the Fabio Baudrit Agricultural Research Station (FBS) and Laguna de Fraijanes Agricultural Research Station (LFS), and in a privately-owned coffee and orange plantation in Grecia canton (Coope-Victoria Farm). Female medfly attractants tested were three food based `female` attractants (FA-3), namely ammonium acetate (AA), 1,4 diaminobutane (putrescine) and trimethylamine, all formulated to last at least one month. These attractants were evaluated either in combinations of two (AA + putrescine, termed FA-2) or all three (termed FA-3). The attractants were tested in various traps including the plastic International Pheromone`s McPhail traps (IPMT) and Tephri traps, a Spanish trap similar to the IPMT. Traps were used either as a dry trap (provided with DDVP) or a wet trap (provided with water and 0.01% surfactant). Jackson traps with Trimedlure (JT,TML), a routinely used male medfly trapping system, was also used. Trapping experiments conducted in the citrus plantation of the FBS resulted in the following fly/trap/day indices (F/T/D): 7.18 with JT,TML; 4.62 with open bottom dry traps (OBDT) baited with FA-3; 4.18 with OBDT (PVC) baited with FA-3; 7.73 with IPMT baited with FA-3, wet; 8.245 with IPMT baited with FA-3, dry; 5.27 with IPMT baited with NuLure; 4.79 with Tephri baited with FA-3, wet; and 5.42 with Tephri, FA-3, dry. The F/T/D indices at the Coope-Victoria Farm, in coffee

Sterile insect technique: new technology to control fruit flies, Ceratitis capitata, in the Lower Basin of the Sao Francisco Valley; Tecnica do inseto esteril: nova tecnologia para combater a mosca-das-frutas, Ceratitis capitata, no Submedio do Vale do Sao Francisco

Energy Technology Data Exchange (ETDEWEB)

Paranhos, Beatriz Aguiar Jordao; Barbosa, Flavia Rabelo [Embrapa Semi-Arido, Petrolina, PE (Brazil); Nascimento, Antonio Souza do [Embrapa Mandioca e Fruticultura, Cruz das Almas, BA (Brazil); Viana, Rodrigo; Malavasi, Aldo [Moscamed Brasil, Juazeiro, BA (Brazil); Sampaio, Raimundo [Agencia Estadual de Defesa Agropecuaria da Bahia (ADAB-BA), Salvador, BA (Brazil); Walder, Julio Marcos Melges [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

2008-12-15

The SIT is the creation, on a large scale, the insect-pest to be controlled and weekly release of these insects sterilized in the field.The sterile insects copulate with the wild, but do not generate descendants. The basic premises for the use of SIT in insect control are: the reproduction is through sexual intercourse, the female copulate preferably only once there is ease of creation of the plague in industrial scale in artificial diet. The efficiency of the SIT may be greater when only the males are released in the field because they increase the probability of copulating with wild females only, with reductions in the cost of production and release. In the case of fruit-flies, sterile females continue doing puncture in the fruits, which decreases the quality for export. To be able to release only males in the field, in 1980s, was developed a mutant strain, whose females emerge from pupae white, thus being able to discard the white ones, keeping the pupae Brown for the release of sterile males. Ten years after, to save on the industrial scale production system, was obtained a mutant whose females possess lethal temperature sensitivity of 34 deg C, still in the embryo stage. Then the eggs are placed on artificial diet, and when they arrive at the pupa stage, they are all brown and males. Forty-eight to 24 hours before the emergence of adults, the pupae are painted with fluorescent powder paint, bagged and irradiated with gamma radiation of 95Gy of Co-60 or X-ray. As soon as the males emerge, are marked with fluorescent ink and when they reach 3 to 5 days old, are released into the field. Thus, when monitoring is done in Jackson traps in the field, it is possible to distinguish wild male sterile under black light or epifluorescence microscope with males, because the sterile are fluorescent. On application of the SIT to Moscamed, sterile males are released in the field must display good dispersibility, good survival and good sexual performance. The efficiency and

Study of genetic variation at the level of DNA between normal and sterilized potato tuber moth males (phthorimaea operculella, zeller)

International Nuclear Information System (INIS)

Ammouneh, H.; Makee, H.; Idris, E.

2009-06-01

The main objective of current study was to determine the genetic variation at the level of DNA between the normal and sterilized potato tuber moth males. The required mating experiments were carried out to obtain partially sterilized males at 150 Gy and their offspring. Some biological factors of these males were estimated and compared to their unirradiated siblings. To acquire good quality and quantity of DNA for the AFLP technique, several methods were undertaken to isolate DNA of potato tuber moth male from different developmental stages especially from adult stage. However, a method called Modified M5 was used. By Modified M5, which required phenol for the isolation of DNA, in some samples 8 to 12 μg were obtained from one insect with 95-100% purity. In our study, AFLP technique was used to analyze the DNA samples of irradiated and unirradiated males from required mating. The result analysis showed that there was a clear genetic variability between examined individuals. However, the relationship between unirradiated F1 and F2 males was stronger than that between irradiated males. Thus, our result demonstrates that the AFLP could be efficiently utilized for studying genetic variation between insect species or between individuals of the same line which have biological differences induced by several factors such as irradiation. Moreover, this technique could enhance the effectiveness of sterile insect technique by using the AFLP in the monitoring system of released insects. (author)

Study Of Genetic Variation At The Level Of DNA Between Normal And Steriled Potato Tuber Moth Males (Phthorimaea operculella, Zeller)

International Nuclear Information System (INIS)

Ammouneh, H.; Makee, H.; Idris, E.

2010-01-01

The main objective of current study was to determine the genetic variation at the level of DNA between the normal and sterilized potato tuber moth males. The required mating experiments were carried out to obtain partially sterilize males at 150 Gy and their offspring. Some biological factors of these males were estimated and compared to their unirradiated siblings. To acquire good quality and quantity of DNA for the AFLP technique, several methods were undertaken to isolate DNA of potato tuber moth male from different developmental stages especially from adult stage. However, a method called Modified M5 was used. By Modified M5, which required phenol for the isolation of DNA, in some samples 8 to 12 Mg were obtained from one insect with 95-100% purity. In our study, AFLP technique was used to analyze the DNA samples of irradiated and unirradiated males from required mating. The result analysis showed that there was a clear genetic variability between examined individuals. However, the relationship between unirradiated F1 and F2 males was stronger than that between irradiated males. Thus, our result demonstrates that the AFLP could be efficiently utilized for studying genetic variation between insect species or between individuals of the same line which have biological differences induced by several factors such as irradiation. Moreover, this technique could enhance the effectiveness of sterile insect technique by using the AFLP in the monitoring system of released insects. (author)

X-ray sterilization of insects and microorganisms for cultural heritage applications

Science.gov (United States)

Borgognoni, F.; Vadrucci, M.; Bazzano, G.; Ferrari, P.; Massa, S.; Moretti, R.; Calvitti, M.; Ronsivalle, C.; Moriani, A.; Picardi, L.

2017-09-01

performed on pest organisms as well as on woods mock-ups to evaluate potential damage to the material during the sterilization. The growing capacity of the treated bacterial cells re-cultured at the end of the treatment was evaluated on the bacterial sample and resulted to strongly inhibit cell growth during post-irradiation incubation, so that after incubation periods at 28 °C, no significant cell growth was observed. The induced levels of insect mortality and sterility vs absorbed dose and operative conditions have been also evaluated, demonstrating the induction of full sterility since the lower dose and 40% mortality by two days after the higher dose treatment. The experiments proved the ability to efficaciously treat objects of cultural heritage with X-rays in order to prevent the increase of the biodeterioration without damaging the materials: in fact, mechanical tests on both irradiated and not irradiated woods have demonstrated the absence of any induced degradation after the radiation exposition.

Quality Control for Expanded Tsetse Production, Sterilization and Field Application

International Nuclear Information System (INIS)

2012-07-01

The use of the sterile insect technique (SIT) for the control of pest insects as part of an integrated, area-wide approach is widely accepted. Its application for the eradication of different tsetse flies, the vectors of human sleeping sickness and African animal trypanosomosis, is attracting increasing interest. Following several initial demonstrations of the application of the SIT for tsetse control the technique was applied on the island of Unguja, Zanzibar, in the mid-1990s and, as the final component of an integrated control programme, led to the eradication of the only tsetse species on the island, Glossina austeni. This successful programme encouraged a number of countries to embark on projects with an SIT component for tsetse control, most Ethiopia. In 2001 the Pan African Tsetse and Trypanosomosis Eradication Campaign (PATTEC) was launched by the Organization of African Unity (now African Union, AU) and, subsequently, six countries obtained funding from the African Development Bank (AfDB) in 2005 to support control programmes with an SIT component. A further six countries have subsequently requested AfDB funding for their programmes. The FAO/IAEA coordinated research project (CRP) on Automation for Tsetse Mass Rearing For Use in Sterile Insect Technique Programmes, which was completed in 2001, led to the development of a semi-automated system for tsetse production. Using this new system, a large rearing facility was established in Addis Ababa, Ethiopia, to supply sterile males for an elimination programme in the southern Rift Valley. The development of large-scale rearing highlighted the need for improved quality control procedures and, with this in mind, the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture established a CRP in 2003 entitled Improved and Harmonized Quality Control for Expanded Tsetse Production, Sterilization and Field Application with the Objective of Improving and Expanding the Quality Control Sections of the FAO

Quality Control for Expanded Tsetse Production, Sterilization and Field Application

Energy Technology Data Exchange (ETDEWEB)

NONE

2012-07-15

The use of the sterile insect technique (SIT) for the control of pest insects as part of an integrated, area-wide approach is widely accepted. Its application for the eradication of different tsetse flies, the vectors of human sleeping sickness and African animal trypanosomosis, is attracting increasing interest. Following several initial demonstrations of the application of the SIT for tsetse control the technique was applied on the island of Unguja, Zanzibar, in the mid-1990s and, as the final component of an integrated control programme, led to the eradication of the only tsetse species on the island, Glossina austeni. This successful programme encouraged a number of countries to embark on projects with an SIT component for tsetse control, most Ethiopia. In 2001 the Pan African Tsetse and Trypanosomosis Eradication Campaign (PATTEC) was launched by the Organization of African Unity (now African Union, AU) and, subsequently, six countries obtained funding from the African Development Bank (AfDB) in 2005 to support control programmes with an SIT component. A further six countries have subsequently requested AfDB funding for their programmes. The FAO/IAEA coordinated research project (CRP) on Automation for Tsetse Mass Rearing For Use in Sterile Insect Technique Programmes, which was completed in 2001, led to the development of a semi-automated system for tsetse production. Using this new system, a large rearing facility was established in Addis Ababa, Ethiopia, to supply sterile males for an elimination programme in the southern Rift Valley. The development of large-scale rearing highlighted the need for improved quality control procedures and, with this in mind, the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture established a CRP in 2003 entitled Improved and Harmonized Quality Control for Expanded Tsetse Production, Sterilization and Field Application with the Objective of Improving and Expanding the Quality Control Sections of the FAO

Influence of ionizing radiation on flight activity of F-1 progeny of sub-sterilized male moths of Spodoptera litura (fabr.) (lepidoptera: noctuidae)

International Nuclear Information System (INIS)

Zubeda; Zarin, Mahtab; Seth, R.K.; Seth, Ranjana

2012-01-01

Radiation mediated 'Inherited or F-1 sterility', as a parabiological (genetic) control measure using a range of 100-130 Gy to the male parents, has been proposed for the suppression of Spodoptera litura (Seth and Sehgal 1993; Seth and Sharma 2001). This is a modified Sterile Insect Technique (SIT) wherein sub-sterilizing gamma doses are preferred than the high (100%) sterilizing dose in order to sustain the insects competitiveness. In the present study the flight activity of F-1 progeny of sub-sterilized male moths was ascertained to judge the performance of F-1 sterility as a control tactic. The male flight ability of F-1 progeny males derived from sub-sterilized male moths, S. litura in presence of normal females, was tested in two modes in 'Flight assay chamber' (a perspex cage of size, 45 x 45 x 60 cm) having a black hollow cylinder (25.4 cm dia, 30.5 cm ht.) placed inside

Wing Morphometry and Acoustic Signals in Sterile and Wild Males: Implications for Mating Success in Ceratitis capitata

Directory of Open Access Journals (Sweden)

João Maria Gomes Alencar de Souza

2015-01-01

Full Text Available The sterile insect technique (SIT is widely utilized in the biological control of fruit flies of the family Tephritidae, particularly against the Mediterranean fruit fly. This study investigated the interaction between mating success and morphometric variation in the wings and the production of acoustic signals among three male groups of Ceratitis capitata (Wiedemann: (1 wild males, (2 irradiated with Co-60 (steriles, and (3 irradiated (steriles and treated with ginger oil. The canonical variate analysis discriminated two groups (males irradiated and males wild, based on the morphological shape of the wings. Among males that emit buzz signals, wild males obtained copulation more frequently than males in Groups 2 and 3. The individuals of Group 3 achieved more matings than those in Group 2. Wild males displayed lower pulse duration, higher intervals between pulses, and higher dominant frequency. Regarding the reproductive success, the morphological differences in the wings’ shape between accepted and nonaccepted males are higher in wild males than in the irradiated ones. The present results can be useful in programs using the sterile insect technique for biological control of C. capitata.

Genetic sexing strains for four species of insects

International Nuclear Information System (INIS)

Seawright, J.A.

1990-01-01

Genetic sexing strains were assembled by using classical genetic and cytogenetic techniques for four medically important species of insects. Male linked reciprocal translocations were used to impose pseudolinkage of sex and selectable genes (shown in parentheses) for Anopheles albimanus (propoxur resistance), Anopheles quadrimaculatus sp. A (malathion resistance), Stomoxys calcitrans (dieldrin resistance; malathion resistance, and black pupa), and Musca domestica (black pupa). These strains would be of great value in implementation of the sterile insect technique for control of these species because the females can either be killed in the egg stage (in the case of insecticide resistance as the selectable gene) or they can be separated from the males and thus excluded from releases. (author). 13 refs

Developing methods of measuring competitiveness of sterilized laboratory insects. Part of a coordinated programme on the use of the sterile male technique for control of Lepidopterous insects attacking fruit and forest trees

International Nuclear Information System (INIS)

Szalay-Marzso, L.

1978-08-01

Optical and acoustical equipment were developed and tested for measuring insect activity, one of the parameters used to assess insect quality. The acoustical equipment which recorded the sound generated by moving insects performed well in the laboratory but was either unsuitable to measure the activity of populations as opposed to pairs or was unsuitable under field conditions. The optical equipment, however, based on light interception, performed well in the field also. In other studies the fitness of insects receiving various treatments was assessed on the basis of release-recapture experiments, number of matings and percentage sperm transfer. These methods proved very sensitive but were more laborious compared to automated recordings

Insects, isotopes and radiations

International Nuclear Information System (INIS)

Lingkvist, D.A.

1987-01-01

The IAEA activity on coordinating the IAEA member-state efforts in the field of pest control is considered. A complex program of agricultural pest control (IPM), applied in many parts of the world is developed. The program provides for the use of natural means of control and cases of critical pest numbers-the use of insecticides. When controlling certain types of insects it is advisable to apply the 'large area control' methods which provide for the insect destruction in places of their concentration prior to migration. Methods of pest control over large areas also include radiation sexual sterilization method (SSM), application of insect phoromons (sexual attractants) to prevent mating, other types of chemical attractants, traps, mass cultivation and reproduction of parasite plants and animals, destroying insects, as well as improvement of host-plant resistance. A great attention is paid to isotope and radiation application in pest control (labelling, sexual sterilization using ionising radiation, radiation application in genetic engineering, mutant plant cultivation)

Potential for control of Cadra cautella (Walker) by release of fully or partially sterile males

International Nuclear Information System (INIS)

Brower, J.H.; Tilton, E.W.

1975-01-01

Modifications of the sterile-male release technique appear to offer promise for the control of post-harvest lepidopteran pest populations within storage structures. Theoretical models of release strategies for Cadra cautella showed that each method has an 'ideal' flooding ratio. This ratio represents the best balance between extinction in a few generations and minimization of the total number of insects released. From data reported concerning the population dynamics of Cadra cautella in the field an ideal flooding ratio for fully sterile (S) males to fertile (F) males of 97:1 would achieve extinction in 3 generations. Total numbers of males released per 100 native insects that would be needed to achieve extinction at these flooding ratios would be 14,850, 14,900 and 18,500, respectively. (author)

Studies on the ecology of insects sterilized artificially (gamma radiation), 7

International Nuclear Information System (INIS)

Kiyoku, Masao; Tsukuda, Ritsuko

1974-01-01

Larvae of an oriental fruit fly line introduced, from Amami-Oshima island were reared using an artificial medium prepared with the base ingredient as corn-flour. When pupae were irradiated with a dose of 8 kR from the 137 Cs as gamma-rays source two days before emerging, both male and female adults emerged showed complete sterility. Percentage in emergence, however, was not significantly reduced. In an experiment that two hundred sterilized plus ten normal males were confined with ten normal females in a mating cage, the sterilized males were fully competitive with normal ones in mating to the normal females. Longevity of the sterilized adult males, however, tended to be slightly shorter than that of the normal ones when compared in the survival curves. When less than one hundred and fifty sterilized males were confined, however, few larvae were able to grow to adults. These progeny adult males and females showed the inherited sterility. When dose was 6 kR there was neither significant difference in the longevity between sterilized and normal adults, nor the complete sterility. Besides, the inherited sterility in offspring was not shown clearly. When more than two hundred sterilized plus ten normal males were combined with ten normal females, however, a considerable sterility was found. Causes of the sterility were investigated by dissection of the testes and spermathecae in females mated with 8 kR-sterilized males. (author)

Feasibility of eradicating Ceratitis spp. fruit flies from the Western Cape of South Africa by the sterile insect technique

International Nuclear Information System (INIS)

Barnes, Brian N.; Eyles, David K.

2000-01-01

Fruit production (deciduous fruit, table and wine grapes, and citrus) is a major export-based industry in the Western Cape, with more than 200,000 ha under cultivation. The gross value of these fruits (excluding wine) exceeds US$400 million per annum. Deciduous fruit and table grapes make up the major portion of the industry, with approximately 110,000 ha under production. The Western Cape is host to two species of fruit flies, the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and the Natal fruit fly, C. rosa Karsch. One or both of these species attack at least 19 species of fruits in this area. Both species have very similar life cycles and habits, and can cause enormous crop losses especially to fruits, but also to some vegetables. Both commercial and resource-limited farmers are affected by fruit flies. Control of fruit flies is currently based on ground applications of insecticides, either as full-cover foliar sprays or low-volume bait sprays. Control costs and crop losses for deciduous fruit and table grapes alone are estimated at US$4 million annually. South Africa is the only southern hemisphere deciduous fruit-exporting country that is not fruit fly-free or is not currently engaged in a project to eradicate fruit flies. Unless similar steps are taken, this situation is likely to threaten the competitiveness of the Western Cape's industry. The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture of the United Nations recently approved funding for the INFRUITEC Centre of the ARC-Fruit, Vine and Wine Research Institute in Stellenbosch to undertake a study to determine the feasibility of eradicating fruit flies from the Western Cape by the sterile insect technique (SIT). Most fruits in the Western Cape are produced in valley systems, and many valleys are isolated from one another to a greater or lesser extent. The Western Cape is itself well isolated from other areas with fruit fly hosts: by the ocean on the eastern, southern and

Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance.

Science.gov (United States)

Ben Ami, Eyal; Yuval, Boaz; Jurkevitch, Edouard

2010-01-01

The sterile insect technique (SIT) is a method of biological control whereby millions of factory reared sterile male insects are released into the field. This technique is commonly used to combat the Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae). Sterile medfly males are less competent in attracting and mating with wild females, a property commonly linked to the irradiation process responsible for the sterilization. As bacteria are important partners in the fly's life cycle, we used molecular analytical methods to study the community structure of the gut microbiota in irradiated male medflies. We find that the sterilizing irradiation procedure affects the gut bacterial community structure of the Mediterranean fruit fly. Although the Enterobacteriaceae family remains the dominant bacterial group present in the gut, the levels of Klebsiella species decreases significantly in the days after sterilization. In addition, we detected substantial differences in some bacterial species between the mass rearing strain Vienna 8 and the wild strain. Most notable among these are the increased levels of the potentially pathogenic species Pseudomonas in the industrial strain. Testing the hypothesis that regenerating the original microbiota community could result in enhanced competitiveness of the sterile flies, we found that the addition of the bacterial species Klebsiella oxytoca to the postirradiation diet enables colonization of these bacteria in the gut while resulting in decreased levels of the Pseudomonas sp. Feeding on diets containing bacteria significantly improved sterile male performance in copulatory tests. Further studies will determine the feasibility of bacterial amelioration in SIT operations.

Chilled packing systems for fruit flies (Diptera: Tephritidae) in the sterile insect technique

International Nuclear Information System (INIS)

Hernandez, Emilio; Escobar, Arseny; Bravo, Bigail; Montoya, Pablo; Secretaria de Agricultura, Ganaderia, Desarrollo Rural, Pesca y Alimentacion

2010-01-01

We evaluated three packing systems (PARC boxes, 'GT' screen towers and 'MX' screen towers) for the emergence and sexual maturation of sterile fruit flies, at three adult fl y densities (1, 1.2 and 1.3 fly/cm 2) and three food types. At the lowest density, results showed no significant differences in the longevity and flight ability of adult Anastrepha ludens (Loew) and Anastrepha obliqua Macquart among the three packing systems. Higher densities resulted in a decrease in these parameters. In the evaluation of the three food types, no significant differences were found either on longevity or flight ability of A. ludens. However, the greatest longevity for both sexes A. obliqua was obtained with commercial powdered Mb and the mix of sugar, protein and corn starch on paper (SPCP) food types. The highest value for flight ability in A. obliqua males was obtained with powdered Mb and SPCP food types, and for females with Mb powdered food. Our data indicated that GT and MX screen tower packing systems are an alternative to the PARC boxes, since they were suitable for adult fl y sexual maturation without any harm to their longevity or flight ability. The tested foods were equivalent in both fruit fl y species, with the exception of the agar type for A. obliqua, which yielded the lowest biological parameters evaluated. Our results contribute to the application of new methods for the packing and release of sterile flies in large-scale programs. (author)

Development of female medfly attractants to support the sterile insect technique: experiments conducted in Madeira, Portugal

International Nuclear Information System (INIS)

Pereira, R.

1999-01-01

With the availability of genetic sexing strains of Mediterranean fruit fly, Ceratitis capitata (Wied.), it is possible to release only sterile males in SIT programs. The availability of a new female medfly attractant can reduce labor costs because program progress can be monitored by trapping females instead of the usual male trapping with its labor intensive identification of sterile and wild males. Three Madeira studies evaluated new female attractants; ammonium acetate, putrescine and trimethylamine. One study was carried out in the same area during two different periods of 1996 (8 June to 27 July and 12 October to 30 November). A second study was conducted between 24 May to 05 July, 1997, in two different areas at the same altitude. One area had a wild fly population (sex ratio 1: 1) and the other was in an area where sterile males were released (sex ratio > 9: 1). The third study, conducted from 18 October to 29 November, 1997, compared wild fly captures at low elevations (80 m) with those at high elevations (700 m). The first study showed that the inclusion of the attractant trimethylamine significantly increased the wild female medfly. The percentage of medfly females in the traps with the two and the three attractants (FA-2 and FA-3) was more than 70%. In the second and third studies, the dry traps were more effective than wet traps in capturing wild medfly females. In areas with only wild females, the percentage of females captured was more than 62%. In areas where sterile males were released, the percentage of females captured was between 12% and 19%. In conclusion, the new attractants captured high percentages of females and, when combined with medfly genetic sexing strains, can reduce program costs significantly. (author)

Chilled packing systems for fruit flies (Diptera: Tephritidae) in the sterile insect technique

Energy Technology Data Exchange (ETDEWEB)

Hernandez, Emilio; Escobar, Arseny; Bravo, Bigail; Montoya, Pablo [Instituto Interamericano de Cooperacion para la Agricultura (IICA), Chiapas (Mexico); Secretaria de Agricultura, Ganaderia, Desarrollo Rural, Pesca y Alimentacion (SAGARPA), Mexico, D.F. (Mexico). Programa Moscafrut

2010-07-15

We evaluated three packing systems (PARC boxes, 'GT' screen towers and 'MX' screen towers) for the emergence and sexual maturation of sterile fruit flies, at three adult fl y densities (1, 1.2 and 1.3 fly/cm 2) and three food types. At the lowest density, results showed no significant differences in the longevity and flight ability of adult Anastrepha ludens (Loew) and Anastrepha obliqua Macquart among the three packing systems. Higher densities resulted in a decrease in these parameters. In the evaluation of the three food types, no significant differences were found either on longevity or flight ability of A. ludens. However, the greatest longevity for both sexes A. obliqua was obtained with commercial powdered Mb and the mix of sugar, protein and corn starch on paper (SPCP) food types. The highest value for flight ability in A. obliqua males was obtained with powdered Mb and SPCP food types, and for females with Mb powdered food. Our data indicated that GT and MX screen tower packing systems are an alternative to the PARC boxes, since they were suitable for adult fl y sexual maturation without any harm to their longevity or flight ability. The tested foods were equivalent in both fruit fl y species, with the exception of the agar type for A. obliqua, which yielded the lowest biological parameters evaluated. Our results contribute to the application of new methods for the packing and release of sterile flies in large-scale programs. (author)

Environmental benefits of medfly sterile insect technique in Madeira and their inclusion in a cost-benefit analysis. Study sponsored by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture

International Nuclear Information System (INIS)

2005-11-01

-spectrum insecticides affect access to the market because of the long harvest intervals and residues in fruit. High damage to fruit crops leads to the abandonment of agriculture, which has negative social and environmental consequences. In 1998, the Regional Government of Madeira, with the support of the IAEA and FAO, through a technical cooperation project implemented the Madeira-Med project aimed at controlling the medfly using an integrated approach based on the sterile insect technique (SIT). A mass rearing and sterilization facility with a production capacity of 50 million sterile males per week was built and is currently in operation. Madeira-Med was referred to as an essential stepping-stone for Madeira fruit production to be able to withstand future challenges. The present study sets out to quantify the different categories of benefits that would be obtained by effectively controlling the medfly using SIT and the costs of the control programme. The economic analysis will evaluate how Madeira-Med benefits society as a whole and not only fruit producers. It includes gains from increase in production volumes and the reduction of production costs, which are direct benefits for the farmers. In addition it includes improvements in environmental quality and health that will benefit both farmers and fruit consumers. Recent cost benefit analyses for proposed insect pest eradication or suppression programmes have included some environmental factors, but a systematic valuation of these factors is new to this study

Efficacy Of Some Fungal Isolates And Their Applications For Controlling The Immature Stages Of The Medfly, Ceratitis Capitata (Wiedemann) Before And During Sterile Insect Technique (SIT)

International Nuclear Information System (INIS)

OUDA, S.M.; EL-AKHDAR, E.A.

2009-01-01

The application of the sterile insect technique (SIT) needs a suitable and effective cheap method alternative to chemical pesticides for suppressing or controlling the population density of the Mediterranean fruit fly, Ceratitis capitata (Wi ed.). The effectiveness of five different fungal isolates: Trichoderma longibranchiatum, T. harzianum, Aspergillus terreus, A. niger and Penicillium oxalicum was evaluated against the immature stages of med fly. Filtrate and three spore suspension concentrations (104, 106 and 108 spores/ml) of each fungal isolate were applied to both 3 rd larval instar and pupae at two ages (2 and 8 days-old). Percentage pupation, pupal mortality, adult emergence and sex ratio were studied. The percentage of pupation of 3 rd larval instar was not affected. However, there was a significant increase (P<0.05) in the percentage of pupal mortality as well as in adult emergence from pupae 2 and 8 days-old at all fungal treatments. Pupae (2 days-old) were more susceptible than pupae (8 days-old) to all fungal treatments. The microbial biomass of both A. terreus and P. oxalicum was selected to apply with different concentrations (5, 10 and 20 w/w) on the pupae at the two ages (2 and 8 days-old). Best results were recorded with biomass at 20% concentration of both fungal isolates. Percentage of mortality was significantly higher (P<0.05) than 50% at the two ages (2 and 8 days-old) for both fungi. A significant decrease (P<0.05) was obtained in adult females emergence resulted in a reduction in the number of eggs deposited and subsequently the punctures on fruits. Chitinase enzyme (important for degradation of chitin which is a component of insect cuticle) and cellulase enzyme (used for improving soil fertility) were determined. The microbial biomass by A. terreus was appeared to be safe to human and animals and can be used for combating immature stages of medfly.

Effect of wet bulb depression on heat sterilization time of slash pine lumber

Science.gov (United States)

William T. Simpson

For international trade, heat sterilization of wood products is often required to prevent the spread of insects and pathogens. Application of heat sterilization requires estimates of the time necessary to heat the center of the wood configuration to the temperature required to kill the insect or other pest. The nature of the heating medium was found to have a...

The use of radiation induced sterility for the control of the codling moth. Part of a coordinated programme on the use of the sterile male technique for the control of lepidopterous insects attacking fruit and forest trees

International Nuclear Information System (INIS)

Jermy, T.

1977-12-01

Studies were conducted from 1973 - 1977 in Hungary to obtain background information necessary for the implementation of sterile insect techniques (SIT) and pest management systems. The flight of the codling moth (CM), monitored by sex-pheromone traps, lasted from May to September. The two peaks (corresponding to the two generations per year) and the break between the two flights appeared blurred, i.e. control measures should be carried out nearly continuously during the whole season. The number of CM pairs was estimated by the release-recapture method as 100 to 500 per ha in mid-summer in an untreated orchard. Such naturally occurring low population levels could help initiating successful SIT against the CM even in not thoroughly treated apple stands. Mixed house gardens containing abandoned host trees have to be considered in Hungary as the main reservoir of the supposed ''semiwild type'' CM. Oak forests proved to harbour autonomous ''wild type'' CM populations on wild apple and sorb trees. The chemically well treated apple orchards have generally very low ''farm type'' CM populations. Natural occurrence of the CM granulosis virus in larvae developing in wild apples has been found. Transmission of the virus in laboratory rearings by the mite Tyrophagus putrescentiae was detected. Spraying with virus suspensions fully protected apples against the CM; however, no spread of the virus to neighbouring untreated trees was observed. Surveys for other major insect pests (Lepidoptera, aphids and scales) of apples were also made and the high population levels of several species in the untreated apple stands have been found indicating that (1) natural control generally cannot reduce these populations to an economically acceptable level, and (2) the SIT against the CM must be combined with chemical treatments or with selective methods against these species. Based on a theoretical analysis of economic aspects of the SIT for codling moth control, it was found that in Hungary

Using mass-release of engineered insects to manage insecticide resistance

International Nuclear Information System (INIS)

Alphey, Nina; Coleman, Paul G.; Donnelly, Christl A.

2006-01-01

Transgenic crops expressing insecticidal toxins derived from Bacillus thuringiensis (Bt) are widely used to control insect pests. The benefits of such crops would be lost if resistance to the toxins spread to a significant proportion of the pest population. The main resistance management method, mandatory in the US, is the high-dose/refuge strategy, requiring nearby refuges of toxin-free crops, and the use of toxin doses sufficiently high to kill not only wild type insects but also insects heterozygous for a resistance allele, thereby rendering the resistance functionally recessive. We propose that mass-release of harmless toxin-sensitive insects could substantially delay or even reverse the spread of resistance. Mass-release of such insects is an integral part of RIDL, a genetics-based method of pest control related to the Sterile Insect Technique. We used a population genetic mathematical model to analyze the effects of releasing male insects homozygous for a female-specific dominant lethal genetic construct, and concluded that this RIDL strategy could form an effective component of a resistance management scheme for insecticidal plants and other toxins. (author)

Using mass-release of engineered insects to manage insecticide resistance

Energy Technology Data Exchange (ETDEWEB)

Alphey, Nina [University of Oxford (United Kingdom). Dept. of Zoology; Alphey, Luke [Oxitec Limited, Oxford (United Kingdom); Coleman, Paul G [London School of Hygiene and Tropical Medicine (United Kingdom). Dept. of Infectious and Tropical Diseases; Donnelly, Christl A [Imperial College Faculty of Medicine, London (United Kingdom). Dept. of Infectious Disease Epidemiology

2006-07-01

Transgenic crops expressing insecticidal toxins derived from Bacillus thuringiensis (Bt) are widely used to control insect pests. The benefits of such crops would be lost if resistance to the toxins spread to a significant proportion of the pest population. The main resistance management method, mandatory in the US, is the high-dose/refuge strategy, requiring nearby refuges of toxin-free crops, and the use of toxin doses sufficiently high to kill not only wild type insects but also insects heterozygous for a resistance allele, thereby rendering the resistance functionally recessive. We propose that mass-release of harmless toxin-sensitive insects could substantially delay or even reverse the spread of resistance. Mass-release of such insects is an integral part of RIDL, a genetics-based method of pest control related to the Sterile Insect Technique. We used a population genetic mathematical model to analyze the effects of releasing male insects homozygous for a female-specific dominant lethal genetic construct, and concluded that this RIDL strategy could form an effective component of a resistance management scheme for insecticidal plants and other toxins. (author)

Insect and Pest Control Newsletter. No. 46

International Nuclear Information System (INIS)

1991-08-01

This newsletter lists the FAO/IAEA meetings in the field of pest control held between September 1990 and February 1991 and provides very brief summaries of their contents. It also features a special report on the New World Screwworm in North Africa. An eradication programme, organized by the IAEA and the FAO and based on the sterile insect technique, was implemented, and as a result it is expected that the area will be declared free of the pest during autumn 1991

Heat sterilization times of five hardwood species

Science.gov (United States)

William T. Simpson; Xiping Wang; John W. Forsman; John R. Erickson

2005-01-01

Heat sterilization of lumber, timbers, and pallets is currently used to kill insects, thus preventing their transfer between countries in international trade. An important factor in this treatment is the time required for the center of any wood configuration to reach the temperature necessary to kill the insect. This study explored the effect of size (1-, 1.5-, and 2.0...

In vitro sterilization technique on embryo of black Toraja rice

Science.gov (United States)

Haring, F.; Riadi, M.; Rafiuddin; Sjahril, R.; Muchlis, A. R.

2018-05-01

Toraja black rice has a high anthocyanin content, a water-soluble pigments, with antioxidant activity. Toraja black rice has a variety of seeds colour in one panicles such as full black (the outside and inside the rice), medium black (the outside and slightly inside rice) and a little black (only the outside of rice). Embryo culture in vitro is one way to grow plants in sterile conditions. The presence of contamination and the death of the embryo require in vitro embryo culture. The sterilization technique is a very important first step to eliminate contamination and the death of embryos. This research aims to determine the right material composition for sterilization of black rice’s embryo. The experiment was done by growing black rice on half strength MS media with the treatment of three method of sterilization, i.e.: S1 (70% alcohol for 5 minutes, 3% and 2% Chlorox each for 10 minutes,), S2 (70% alcohol for 3 minutes, 2% Clorox for 10 minutes) and S3 (70% alcohol for 3 minutes and 1% Clorox for 15 minutes). The materials used are rice seedlings that have been cut in two and opened the pericarp of paddy grain, leaving a piece of rice that has a complete embryo. The best sterilization for Toraja black rice embryo culture was using the S3 composition. Best germination was seen on the seeds with full and medium black color.

Sterilization of boll weevil pupae with fractionated doses of gamma irradiation

International Nuclear Information System (INIS)

Haynes, J.W.; Mitlin, N.; Davich, T.B.; Dawson, J.R.; McGovern, W.L.; McKibben, G.H.

1977-01-01

Fractionated doses of 6,250-8,000 rads of gamma irradiation administered to pupae of the boll weevil, Anthonomus grandis Boh., sexually sterilized both sexes. Mortality of males thus treated with 6,250 and 8,000 rads via fractionation was 14% and 27% respectively, by 5 days posttreatment compared with 46% mortality when an equivalent acute dose was administered to newly emerged adults. Pheromone production of males irradiated at 6,250 rads was one-third that of the control for the first 4 days, but equal that of the control during 5-11 days posttreatment. This procedure lends itself to the large-scale sterilization of weevils needed in an eradication program. This technique is applicable to other insects that are highly susceptible to acute doses

Studies on the ecology of insects sterilized artificially (gamma radiation), 8

International Nuclear Information System (INIS)

Kiyoku, Masao; Tsukuda, Ritsuko

1974-01-01

Gamma-ray irradiation of 7 KR from 137 Cs on the oriental fruit fly pupae two days before emerging did not significantly reduce the percentage of emergence and longevity of adult flies. However, highly sterile adults (more than 98.9%) were found in those combinations, S#male# x N#female#, N#male# x S#female# and S#male# x S#female#. The result of observation of sperm activity in spermathecae of females mated with sterilized males showed that the sterility is caused by dominant lethalities. The sexual competitiveness between S#male# and N#male# has been examined in experiments of S#male# x N#male# x N#female#. (1) The data suggest that the sterility percentage concerning with egg hatchability is proportional to the rate of sterilized males replaced for normal males when that is expressed with a multiple of the ratio of normal males to normal females. Seven such straight lines thus obtained made us possible to find the theoretical expectation of sterility percentage. (2) Relationship between the percent egg hatch observed (y), or that estimated theoretically (Y), and logarithm of ratio, S#male#/N#male# (x) was showed to be two straight regression equations, y=18.413-11.952 (x - 1.295) and Y=11.890-15.462 (x - 1.295). Ratio of regression coefficients of the equations was 0.772. (3) Correlations between the sterility percentage and ratio, S#male#/N#male#, showed in three convex curves under three degrees of competitiveness (0.265, 0.576 and 1.001). When both the values of S#male#/N#male# in the mating experiments and the sterility percentages are given, it is possible to obtain degrees of competitiveness using the curves. (auth.)

Flightless mutants in the melon fly and oriental fruit fly (Diptera: Tephritidae) and their possible role in the sterile insect release method

International Nuclear Information System (INIS)

McCombs, S.D.; Saul, S.H.

1992-01-01

Two new mutants that affect adult wing morphology and render the flies incapable of flight.sbd.bubble wing (bw) in the melon fly, Bactrocera cucurbitae (Coquillett), and small wing (sw) in the oriental fruit fly, Bactrocera dorsalis (Hendel).sbd.are described. Both mutants have variable expression and are caused by autosomal, recessive genes. We discuss the possible role of these alleles in constructing genetic sex sorting systems to improve the effectiveness and efficiency of the sterile insect release method

Radiation sterilization facility for melon fly

International Nuclear Information System (INIS)

Danno, A.

1985-01-01

The melon fly (Dacus cucurbitae Coquillett) has been observed in Amami Island since l975. Kagoshima Prefecture has had a melon fly eradication project underway since 1979. A mass-fearing facility and a radiation sterilization facility were constructed in Naze in March of l98l. In the early stages of the project, sterile insects were produced at the rate of 4 x l0/sup 6/ pupae/week. In the later stages, the activity of the project was enlarged by tenfold. The conditions for design of the radiation sterilization facility, which has been developed with a central control system for automated irradiation, are examined from an engineering standpoint

Pre-Release Consumption of Methyl Eugenol Increases the Mating Competitiveness of Sterile Males of the Oriental Fruit Fly, Bactrocera dorsalis, in Large Field Enclosures

Science.gov (United States)

Shelly, Todd E.; Edu, James; McInnis, Donald

2010-01-01

The sterile insect technique may be implemented to control populations of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), when environmental concerns preclude widespread use of chemical attractants or toxicants. The goal of the present study was to evaluate whether the mating competitiveness of sterile B. dorsalis males could be increased via pre-release feeding on methyl eugenol. Males of the oriental fruit fly are strongly attracted to this plant-borne compound, which they ingest and use in the synthesis of the sex pheromone. Previous studies conducted in the laboratory and small field-cages have shown that males given methyl eugenol produce a more attractive pheromone for females and have a higher mating success rate than males denied methyl eugenol. Here, levels of egg sterility were compared following the release of wild-like flies and either methyl eugenol-fed (treated) or methyl eugenol-deprived (control) sterile males in large field enclosures at four over flooding ratios ranging from 5:1 to 60:1 (sterile: wild-like males). Treated sterile males were fed methyl eugenol for 1–4 h (depending on the over flooding ratio tested) 3 d prior to release. Eggs were dissected from introduced fruits (apples), incubated in the laboratory, and scored for hatch rate. The effect of methyl eugenol was most pronounced at lower over flooding ratios. At the 5:1 and 10:1 over flooding ratios, the level of egg sterility observed for treated, sterile males was significantly greater than that observed for control, sterile males. In addition, the incidence of egg sterility reported for treated sterile males at these lower over flooding ratios was similar to that noted for treated or control sterile males at the 30:1 or 60:1 over flooding ratios. This latter result, in particular, suggests that pre-release feeding on methyl eugenol allows for a reduction in the number of sterile flies that are produced and released, thus increasing the cost

Genetic control of fruit flies

International Nuclear Information System (INIS)

Walder, J.M.M.

1987-01-01

The sterile-insect technique for control of fruit-flies is studied. A brief historic of the technique is presented, as well as a short description of the methodology. Other aspects are discussed: causes of sterility in insects and the principles of insect population suppression by sterile-insect technique. (M.A.C.)

Testosterone as a Bio marker for Rapid Detection of Male Sterility of the Mediterranean Fruit Fly, Ceratitis Capitata (Wied.)

International Nuclear Information System (INIS)

Shoman, A.A.; El-Arab, A.E.; Aly, M.S.A.

2000-01-01

A rapid biochemical method for the determination of the sterility in males of the fruit fly, Ceratitis Capitata (Wied.) was established utilizing testosterone as a bio marker. Pupae (2 days before emergence) were irradiated at 90 Gy using a 60 Co-gamma-cell, unit. Testosterone level, measured by radioimmunoassay (RIA), in addition to sterility in irradiated male flies were compared to their corresponding values of the untreated control group. The data obtained revealed a highly significant decrease in the testosterone level associated with a highly significant decrease in sterility. The decrease in the hormone level was recognized during the different time intervals (24-120 hours after adult emergence). This bio marker (Testosterone) could be adopted as a rapid monitor for measurements of medfly male sterility before release in the field in a sterile insect technique (SIT) program

Inheritance of sterility in Dysdercus koenigii F. (Hemiptera: Pyrrhocoridae)

International Nuclear Information System (INIS)

Harwalkar, M.R.; Rahalkar, G.W.

1979-01-01

It has been suggested that for the control of Lepidopterous populations, release of fully competitive partially sterile males would be more advantageous than the release of completely sterile males. This suggestion is based on the fact, elucidated in many studies, that partially sterilized males mated to normal females produce totally sterile or partially sterile progeny. In Hemiptera too, F 1 progeny of partially sterile males has been shown to inherit sterility. Individual impact of sterility inherited by either sex of the F 1 progeny on population growth has been studied in an hemipteran insect Dysdercus koenigii. A dose of 7 krad induces near-complete sterility in males. When males irradiated with substerilizing doses of 1,2 and 3 krad were crossed with normal females, F 1 progeny of both sexes was partially sterile : the female being more sterile than the males. When the F 1 progeny was intercrossed, there was enhanced reduction in progeny production. (auth.)

Evaluating the potential of suppressing codling moth, Cydia pomonella L. field populations by the use of the sterile insect release method

International Nuclear Information System (INIS)

Mansour, M.

1998-11-01

Studies were done to evaluate the possibility of using the sterile insect technique for codling moth, Cydia pomonella L., eradication from Syria. Data accumulated during these studies indicate that codling moth has two distinct generations (spring and summer generation) in the country. Spring generation starts from mid April to early May, reaches its peak about one month later, then declines rapidly soon after. Adults of summer generation start their activity in July, peak in August and wild moths disappear by late September to early October. A very small percentage of spring generation larvae enter diapause, while most of summer generation larvae and all of hose maturing in September do so. Sex ratio is about 1:1 (males to females). Studies on population density and rate of increase show that it ranged between 2.4 and 11.05 larvae/tree in commercial and neglected apple orchards respectively. The results also indicate that codling moth summer generation increased about three times more than spring generation. In the radiosensitivity studies, it is clear that egg production and hatch decreased with increasing radiation dose. Females were more sensitive to irradiation treatment than males. A dose of 15 krad caused 100% sterility in treated females and reduced fecundity to less than 1% This dose (35 Krad) had no adverse effect on male longevity and competitiveness under laboratory conditions. However, males exposed to a dose of 25 or 35 Krad mated less times than control males and recovered some of their fertility overtime. (author)

Potential use of the sterile insect technique against the South American fruit fly

International Nuclear Information System (INIS)

Ortiz, G.

1999-01-01

The Latin American countries have a strong interest in increasing fruit production and quality to facilitate commercialization within and outside the region. Various fruit fly control programmes in South America and their objectives and benefits are described here. Specific priorities to improve fruit fly control and eradication technologies include strengthening of quarantine, development of pre- and post-phytosanitary measures, and harmonization of the most effective and advanced technical procedures/methodologies to control fruit flies. A subregional strategy to control fruit flies in South America would promote technical co-operation among the South American countries and strengthen the activities of less advanced fruit fly programmes. Effective use can be made of local/regional infrastructure, expertise, sterile fly production and human/technical resources. In Argentina, advanced technology related to the use of medfly genetic sexing strains for SIT programmes has been successfully introduced. Joint efforts between technicians and scientists would contribute to developing new technology to control important pests in South America. (author)

Comparative mating and reproductive performance of radiation sterilized and radiation induced F1 sterile males of Earias vittella (Fabricius)

International Nuclear Information System (INIS)

Shantharam, K.; Tamhankar, A.J.; Harwalkar, M.R.

1995-01-01

Studies were conducted on mating behaviour and reproductive performance of a) Earias vittella (F.) males rendered sterile by exposing them to 300 Gy gamma rays and b) F 1 sterile males obtained by exposing parent male to 100 Gy gamma rays. For the study, males were allowed only one mating with a normal female. Results revealed that premating period, period in copula and per cent insects mating were not adversely affected in case of both types of sterile males and fecundity also remained unaffected. However, in both types of sterile males, incidence of spermatophore transfer without sperm was very large and such an occurrence (including reduced spermatophore transfer) was significantly higher in case of radiation sterilized males compared to the F 1 sterile males. Inability to produce and transfer spermatophore and/or sperm appeared to be a major cause behind the reduced mating competitiveness of both types of males. (author). 22 refs., 2 tabs

Ozone-mist spray sterilization for pest control in agricultural management

Science.gov (United States)

Ebihara, Kenji; Mitsugi, Fumiaki; Ikegami, Tomoaki; Nakamura, Norihito; Hashimoto, Yukio; Yamashita, Yoshitaka; Baba, Seiji; Stryczewska, Henryka D.; Pawlat, Joanna; Teii, Shinriki; Sung, Ta-Lun

2013-02-01

We developed a portable ozone-mist sterilization system to exterminate pests (harmful insects) in agricultural field and greenhouse. The system is composed of an ozone generator, an ozone-mist spray and a small container of ozone gas. The ozone generator can supply highly concentrated ozone using the surface dielectric barrier discharge. Ozone-mist is produced using a developed nozzle system. We studied the effects of ozone-mist spray sterilization on insects and agricultural plants. The sterilization conditions are estimated by monitoring the behavior of aphids and observing the damage of the plants. It was shown that aphids were exterminated in 30 s without noticeable damages of the plant leaves. The reactive radicals with strong oxidation potential such as hydroxyl radical (*OH), hydroperoxide radical (*HO2), the superoxide ion radical (*O2‒) and ozonide radical ion (*O3‒) can increase the sterilization rate for aphids. Contribution to the Topical Issue "13th International Symposium on High Pressure Low Temperature Plasma Chemistry (Hakone XIII)", Edited by Nicolas Gherardi, Henryca Danuta Stryczewska and Yvan Ségui.

Evaluation of user input methods for manipulating a tablet personal computer in sterile techniques.

Science.gov (United States)

Yamada, Akira; Komatsu, Daisuke; Suzuki, Takeshi; Kurozumi, Masahiro; Fujinaga, Yasunari; Ueda, Kazuhiko; Kadoya, Masumi

2017-02-01

To determine a quick and accurate user input method for manipulating tablet personal computers (PCs) in sterile techniques. We evaluated three different manipulation methods, (1) Computer mouse and sterile system drape, (2) Fingers and sterile system drape, and (3) Digitizer stylus and sterile ultrasound probe cover with a pinhole, in terms of the central processing unit (CPU) performance, manipulation performance, and contactlessness. A significant decrease in CPU score ([Formula: see text]) and an increase in CPU temperature ([Formula: see text]) were observed when a system drape was used. The respective mean times taken to select a target image from an image series (ST) and the mean times for measuring points on an image (MT) were [Formula: see text] and [Formula: see text] s for the computer mouse method, [Formula: see text] and [Formula: see text] s for the finger method, and [Formula: see text] and [Formula: see text] s for the digitizer stylus method, respectively. The ST for the finger method was significantly longer than for the digitizer stylus method ([Formula: see text]). The MT for the computer mouse method was significantly longer than for the digitizer stylus method ([Formula: see text]). The mean success rate for measuring points on an image was significantly lower for the finger method when the diameter of the target was equal to or smaller than 8 mm than for the other methods. No significant difference in the adenosine triphosphate amount at the surface of the tablet PC was observed before, during, or after manipulation via the digitizer stylus method while wearing starch-powdered sterile gloves ([Formula: see text]). Quick and accurate manipulation of tablet PCs in sterile techniques without CPU load is feasible using a digitizer stylus and sterile ultrasound probe cover with a pinhole.

Reversibility of female sterilization.

Science.gov (United States)

Siegler, A M; Hulka, J; Peretz, A

1985-04-01

The discussion considers the current status of reversibility of sterilization in the US and describes clinical and experimental efforts for developing techniques designed for reversibility. It focuses on regret following sterilization, reversal potential of current sterilization techniques, patient selection, current reversal techniques, results of sterilization procedures, experimental approaches to reversal of current techniques of sterilization, and sterilization procedures devised for reversibility, in humans and in animals. Request is the 1st stage of reversal, but a request for sterilization reversal (SR) does not always mean regret for a decision made at the time. Frequently it is a wish to restore fertility because life circumstances have changed after a sterilization that was ppropriate at the time it was performed. Schwyhart and Kutner reviewed 22 studies published between 1949-69 in which they found that the percentage of patients regretting the procedure ranged from 1.3-15%. Requests for reversal remain low in most countries, but if sterilization becomes a more popular method of contraception, requests will also increase. The ideal operation considered as a reversaible method of sterilization should include an easy, reliable outpatient method of tubal occlusion with miniml risk or patient discomfort that subsequently could be reversed without the need for a major surgical intervention. Endoscopic methods have progressed toward the 1st objective. A recent search of the literature uncovered few series of SR of more than 50 cases. The 767 operations found were analyzed with regard to pregnancy outcome. The precent of live births varied from 74-78.8%, and the occurance of tubal pregnancies ranged from 1.7-6.5%. All of the confounding variables in patient selection and small numbers of reported procedures preclude any conclusion about the different techniques or the number of operations that give a surgeon a level of expertise. Few authors classify their

Developing the F1 sterility technique for the management of the carob moth ectomyelois ceratoniae zeller (Lepidoptera: pyralidae) in a pomegranate orchards

International Nuclear Information System (INIS)

Ben-Jemaa, J. M. M.; Dhouibi, M. E. H.

2012-12-01

A pilot sterile insect release program was initiated for the control of the date moth ectromyelois ceratoniae Zeller (Lepidoptera: Pyralidae) in a pomegranate orchard near Tunisia. The F1 sterility technique was tested against pest for four successive seasons. Moth adults were irradiated with an effective gamma dose of 400 Gy using a 6 0C o irradiator (dose rate of 46 Gy/min). Releases were done from June to September each year with a release ratio of 5 to 1 (irradiated to non-irradiated). The release assessment was measured in the treated field as the reduction of the percentage of fruit damage and the reduction of percentage of larvae in rotten fruits at harvest. Results showed that F1 sterility could be a potential method for the control of carob in pomegranate orcharch. In the treated field, the percent damage of pomegranates at harvest declined from 28% in first year to 6.5% last year in the treated plot against 32% and 33% in the control plots. The percent of rotten fruits in the treated area was respectively 26%, 5.5%, 3.25% and 1.25% against 32%, 30.5% 30% and 30.5% in the control area during 4 years. The percentage of larvae in rotten fruit was respectively 20%, 5.5%, 3.25% and 1.25% compared to 32%, 30.5%, 30% and 30.5% in the controls. (Author)

Life history evolution in social insects : A female perspective

NARCIS (Netherlands)

Negroni, Matteo Antoine; Jongepier, Evelien; Feldmeyer, Barbara; Kramer, Boris H.; Foitzik, Susanne

2016-01-01

Social insects are known for their unusual life histories with fecund, long-lived queens and sterile, short-lived workers. We review ultimate factors underlying variation in life history strategies in female social insects, whose social life reshapes common trade-offs, such as the one between

Insects vis a vis radiations

International Nuclear Information System (INIS)

Srivastava, Meera

2014-01-01

Insects have turned out to be much more radiation resistant. For most insects a dose of about 500-700 Gy is required to kill them within a few weeks of exposure; although cockroaches require 900-1000 Gy. Killing insects in less than a few days requires much higher doses. These doses are for mature insects, the immature stages of some insects can be killed by doses as low as 40 Gy. Some insects can be sterilized at even lower doses, and this has application in insect control. Screw-worms, for example, can be sterilized with doses of 25-50 Gy. By contrast, doses as low as 3 Gy caused death of humans in Hiroshima and Nagasaki and doses of about 6 Gy caused death of fire fighters in the Chernobyl accident. It is not exactly certain what the basis is for the resistance of insects to ionizing radiation. It is not animal size by itself, nor lack of penetration. It is also not because of few dividing cells as these are more radiosensitive than non-dividing ones. The speculation that insects might have lower oxygen tensions, and the lack of oxygen is known to protect cells from radiation also does not work. Insect cells might have an enhanced capacity to repair radiation damage also could not be proven. The number of chromosomes influenced radio-sensitivity, and that insects had fewer chromosomes could be true. The radiation resistance is inherent to the cells, since cells derived from insects are also radiation resistant when grown in cell culture. For example, a dose of 60 Gy is required to produce a 80% kill of insect cells, while doses of 1-2 Gy are sufficient to generate this level of killing in mammalian cells. But, nevertheless, according to recent researches, radiation from Japan's leaking Fukushima nuclear plant has caused mutations in some butterflies. It is therefore clear that insects are resistant to ionizing radiation and that this resistance is an inherent property of their cells. But it is not clear exactly what the basis of this cellular resistance is

The FAO/IAEA interactive spreadsheet for design and operation of insect mass rearing facilities

International Nuclear Information System (INIS)

Caceres, Carlos; Rendon, Pedro

2006-01-01

An electronic spreadsheet is described which helps users to design, equip and operate facilities for the mass rearing of insects for use in insect pest control programmes integrating the sterile insect technique. The spreadsheet was designed based on experience accumulated in the mass rearing of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), using genetic sexing strains based on a temperature sensitive lethal (tsl) mutation. The spreadsheet takes into account the biological, production, and quality control parameters of the species to be mass reared, as well as the diets and equipment required. All this information is incorporated into the spreadsheet for user-friendly calculation of the main components involved in facility design and operation. Outputs of the spreadsheet include size of the different rearing areas, rearing equipment, volumes of diet ingredients, other consumables, as well as personnel requirements. By adding cost factors to these components, the spreadsheet can estimate the costs of facility construction, equipment, and operation. All the output parameters can be easily generated by simply entering the target number of sterile insects required per week. For other insect species, the biological and production characteristics need to be defined and inputted accordingly to obtain outputs relevant to these species. This spreadsheet, available under http://www-naweb.iaea.org/nafa/ipc/index.html, is a powerful tool for project and facility managers as it can be used to estimate facility cost, production cost, and production projections under different rearing efficiency scenarios. (author)

The FAO/IAEA interactive spreadsheet for design and operation of insect mass rearing facilities

Energy Technology Data Exchange (ETDEWEB)

Caceres, Carlos, E-mail: carlos.e.caceres@aphis.usda.co [International Atomic Energy Agency (IAEA), Seibersdorf (Austria). Agency' s Labs. Programme of Nuclear Techniques in Food and Agriculture; Rendon, Pedro [U.S. Department of Agriculture (USDA/APHIS/CPHST), Guatemala City (Guatemala). Animal and Plant Health Inspection. Center for Plant Health Science and Technology

2006-07-01

An electronic spreadsheet is described which helps users to design, equip and operate facilities for the mass rearing of insects for use in insect pest control programmes integrating the sterile insect technique. The spreadsheet was designed based on experience accumulated in the mass rearing of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), using genetic sexing strains based on a temperature sensitive lethal (tsl) mutation. The spreadsheet takes into account the biological, production, and quality control parameters of the species to be mass reared, as well as the diets and equipment required. All this information is incorporated into the spreadsheet for user-friendly calculation of the main components involved in facility design and operation. Outputs of the spreadsheet include size of the different rearing areas, rearing equipment, volumes of diet ingredients, other consumables, as well as personnel requirements. By adding cost factors to these components, the spreadsheet can estimate the costs of facility construction, equipment, and operation. All the output parameters can be easily generated by simply entering the target number of sterile insects required per week. For other insect species, the biological and production characteristics need to be defined and inputted accordingly to obtain outputs relevant to these species. This spreadsheet, available under http://www-naweb.iaea.org/nafa/ipc/index.html, is a powerful tool for project and facility managers as it can be used to estimate facility cost, production cost, and production projections under different rearing efficiency scenarios. (author)

Microbiological Load of Edible Insects Found in Belgium.

Science.gov (United States)

Caparros Megido, Rudy; Desmedt, Sandrine; Blecker, Christophe; Béra, François; Haubruge, Éric; Alabi, Taofic; Francis, Frédéric

2017-01-13

Edible insects are gaining more and more attention as a sustainable source of animal protein for food and feed in the future. In Belgium, some insect products can be found on the market, and consumers are sourcing fresh insects from fishing stores or towards traditional markets to find exotic insects that are illegal and not sanitarily controlled. From this perspective, this study aims to characterize the microbial load of edible insects found in Belgium (i.e., fresh mealworms and house crickets from European farms and smoked termites and caterpillars from a traditional Congolese market) and to evaluate the efficiency of different processing methods (blanching for all species and freeze-drying and sterilization for European species) in reducing microorganism counts. All untreated insect samples had a total aerobic count higher than the limit for fresh minced meat (6.7 log cfu/g). Nevertheless, a species-dependent blanching step has led to a reduction of the total aerobic count under this limit, except for one caterpillar species. Freeze-drying and sterilization treatments on European species were also effective in reducing the total aerobic count. Yeast and mold counts for untreated insects were above the Good Manufacturing Practice limits for raw meat, but all treatments attained a reduction of these microorganisms under this limit. These results confirmed that fresh insects, but also smoked insects from non-European trades, need a cooking step (at least composed of a first blanching step) before consumption. Therefore, blanching timing for each studied insect species is proposed and discussed.

Microbiological Load of Edible Insects Found in Belgium

Directory of Open Access Journals (Sweden)

Rudy Caparros Megido

2017-01-01

Full Text Available Edible insects are gaining more and more attention as a sustainable source of animal protein for food and feed in the future. In Belgium, some insect products can be found on the market, and consumers are sourcing fresh insects from fishing stores or towards traditional markets to find exotic insects that are illegal and not sanitarily controlled. From this perspective, this study aims to characterize the microbial load of edible insects found in Belgium (i.e., fresh mealworms and house crickets from European farms and smoked termites and caterpillars from a traditional Congolese market and to evaluate the efficiency of different processing methods (blanching for all species and freeze-drying and sterilization for European species in reducing microorganism counts. All untreated insect samples had a total aerobic count higher than the limit for fresh minced meat (6.7 log cfu/g. Nevertheless, a species-dependent blanching step has led to a reduction of the total aerobic count under this limit, except for one caterpillar species. Freeze-drying and sterilization treatments on European species were also effective in reducing the total aerobic count. Yeast and mold counts for untreated insects were above the Good Manufacturing Practice limits for raw meat, but all treatments attained a reduction of these microorganisms under this limit. These results confirmed that fresh insects, but also smoked insects from non-European trades, need a cooking step (at least composed of a first blanching step before consumption. Therefore, blanching timing for each studied insect species is proposed and discussed.

Radiation and Radioisotopes Applied to Insects of Agricultural Importance. Proceedings of the Symposium on the Use and Application of Radioisotopes and Radiation in the Control of Plant and Animal Insect Pests

Energy Technology Data Exchange (ETDEWEB)

NONE

1963-09-15

Since the pioneer work of the United States Department of Agriculture in the application of radiation and radioisotopes in the control of insect pests to cattle, many countries and organizations have pursued the advantages which might be gained in this field. Two years ago the IAEA organized the first international symposium in Bombay to study this problem, since when a considerable amount of basic research on the application of nuclear science in entomology and insect pest control has been undertaken. The potential gain of these studies, which would be in the form of an increased output of better food, is obvious to all Governments; hence the extensive international interest in the subject of this present Symposium, which was attended by 100 participants from 26 countries and 5 international organizations. The proceedings consist of 37 papers presented by experts from 10 countries, together with a record of the discussions, and cover the use of radioisotopes in the study of the ecology of insects, such as their dispersal, migration and life-cycle. The application of radioisotopes to insecticides covers such subjects as labelling, application, uptake, translocation, metabolism, mode of action, and the determination' of residues in plants and animals. The present position on the effects of radiation on insects is dealt with, including mutation, sterilization and the use of the sterile-male technique for the control and eradication of insect pests, and the need is emphasized for integration of chemical, biological, radiation and other methods of insect control. The emphasis of this Symposium has been mainly on aspects of crop protection and it is hoped that the next symposium will also deal with aspects of livestock protection.

Use of gamma radiation of Cobalt-60 aiming at the quarentenary treatment and the sterilization of Stenoma catenifer Walsingham (Lepidoptera: Elachistidae)

International Nuclear Information System (INIS)

Silva, L.K.F.; Arthur, V.; Nava, D.E.; Parra, J.R.P.

2007-01-01

The objective of this work was verify the influence of gamma radiation of Cobalt-60, in the avocado moth Stenoma catenifer Walsingham (Lepidoptera: Elachistidae). For this research, insects were irradiated in all phases of their life cycle with doses varying from 0 to 600 Gy and they were maintained at the temperature of 25 +/- 2 deg C, relative humidity of 70 +/- 10% and photofase of 14 h. The species was reared on natural diet, avocado seeds, variety Breda. According to the obtained results, was verified that the lethal dose of gamma radiation to S. catenifer larvae and pupae was of 300 Gy. The sterile dose for upcoming adults from irradiated S. catenifer larvae, was of 100 Gy; for adults coming from irradiated pupas was of 150 Gy and for irradiated adults was of 200 Gy. The use the gamma radiation as treatment quarentine of S. catenifer was efficient, should be treated them with doses able to promote the sterilization of insect, without committing your qualities physical-chemistries and sensorial of fruits. Therefore, is suggested the treatment with the dose of 50 Gy for fruits infested with eggs and 150 Gy for fruits infested with larvae and pupas of S. catenifer. For use the sterile insect technique is recommended the irradiation of pupas and adults with doses of 150 Gy and 200 Gy, respectively [pt

Investigation on mating habit and sexual competition ability of Helicoverpa armigera sterilized by irradiation

International Nuclear Information System (INIS)

Xia Darong; Yang Rongxin; Gu Weiping; Zhang Yanjun

1999-01-01

Mating habit and sexual competition ability of Helicoverpa armigera irradiated with 300 Gy of γ-rays were observed. The results showed that when reared in laboratory, most adults mated at 4-7 o'clock, a small proportion of adults mated at 2-3 o'clock or at 8-9 o'clock in the morning, meanwhile wild population mated two and a half hours earlier than laboratory populations did. No difference in mating habit and sexual competition ability was found between the irradiation-sterilized and normal adults except that the effective mating rate of the former was 61.6%, one third less than that of the latter ones. Most irradiation-sterilized adults mated one time in their whole lives, only a few could mate 2-3 times and even 5 times, indicating that the multi-mating characteristic of Helicoverpa armigera was not changed by irradiation. It can be concluded that irradiated sterile insect technique is feasible for integrated controlling the cotton bollworm

Eradication of the melon fly, Bactrocera cucurbitae Coquillett, by mass release of sterile flies in Okinawa prefecture, Japan

International Nuclear Information System (INIS)

Kakinohana, H.; Kuba, H.; Kohama, T.; Kinjo, K.; Taniguchi, M.; Nakamori, H.; Tanahara, A.; Sokei, Y.

1997-01-01

In 1972, MAFF, Japan and the Okinawa Prefectural Government initiated an experimental eradication project of the melon fly from Kume Island, Okinawa Prefecture, Japan using the sterile insect technique (SIT). Following the successful eradication on Kume Island in 1978, large scale SIT was started to eradicate the melon fly on the 3 groups of islands, Miyako, Okinawa and Yaeyama of Okinawa Prefecture, Japan in 1984, 1986 and 1989, and eradication was achieved in 1987, 1990 and 1993, respectively. For the successful eradication on Miyako, Okinawa and Yaeyama groups of islands, about 6,340, 30,940 and 15,440 million sterile melon flies were released, respectively

Control of the wax moth Galleria mellonella L. (Lepidoptera: Pyralidae by the male sterile technique (MST

Directory of Open Access Journals (Sweden)

Jafari Reza

2010-01-01

Full Text Available In this study we examined the control of wax moth using the male sterile technique (MST with gamma-rays. To determine the safe and effective dosage of gamma-rays capable of sterilizing male pupae of the wax moth, male pupae were exposed to increasing single doses of gamma-rays (250, 300, 350 and 400 Gy. The release ratio of sterile to normal males was also studied in a similar experiment. Treatments included sterile males, normal males and virgin females at the following ratios: 1:1:1, 2:1:1, 3:1:1, 4:1:1 and 5:1:1. Possible parthenogenetic reproduction of this pest was also examined. The results showed that 350 Gy was the most effective dose capable of sterilizing the male pupae of the wax moth. The best release ratio was established at four sterile males, one normal male for each normal female (4:1:1. Also females were incapable of producing offspring without males.

Genetic methods for area-wide management of Lepidopterous pests with emphasis on F1 sterility

International Nuclear Information System (INIS)

Ocampo, V.R.

1996-01-01

Enormous losses in the production and marketing of food and fiber are caused by larvae of Lepidoptera. Currently, large quantities of insecticides are used to combat these pests. Insecticide resistance, increasing concern over pesticide pollution, and the desire to effectively manage lepidopteran pests on an area-wide basis have motivated scientists to identify and develop new pest management tactics that are compatible with current IPM. Genetic methods have emerged as a promising control strategy for lepidopteran pests. Genetic control as a practical means of pest management was first successfully implemented by Knipling and colleagues in the USA during the 1960's with the sterile insect technique (SIT) program for the screwworm fly. SIT is not a readily adapted for use against Lepidoptera as against Diptera. Radiation-induced inherited sterility (or F 1 sterility) is generally considered the most promising genetic methods for large-scale suppression of lepidopteran populations. This papers discusses four genetic control methods that have been developed and the progress that has been made in integrating sterility with other IPM tactics. (author)

Field trials in South China to control the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) using radiation-induced sterility

International Nuclear Information System (INIS)

Yang, R.; Xia, D.; Gu, W.; Zhang, Y.

2002-01-01

This paper discusses the control of the diamondback moth, Plutella xylostella by the sterile insect technique (SIT). Our studies included mating characteristics, sterility of the F 1 generation, dispersal and recapture of irradiated moths, as well as control of DBM using SIT and F 1 sterility, and an economic evaluation of F 1 sterility to protect cabbage. Male DBM mated an average of 16 times and female DBM mated an average of 4 times. However, irradiated male DBM only mated an average of 7.2 times. Seventy percent of matings occurred from 18:00-24:00 h with an average duration of 80 min. Irradiated male moths and untreated male moths exhibited the same attraction to female moths. After 10 days, most (94.2%) of the released, sterile DBM were recaptured within 40 m of the release site. Only one DBM was recaptured at 120 m from the release site. The area of dispersal was calculated to be 696 m 2 during the first three days. In a field study to control DBM by releases of irradiated insects, the ratio of sterile to wild DBM was 4.7:1. During this study, the egg sterility in the F 1 and F 2 generations was 79.0% and 81.7%, respectively. The developmental times for the F 1 and F 2 generations were 4 and 12 days longer, respectively, than for DBM in the control area. Thus, the number of DBM generations was reduced in the treated field. With successive releases over two generations, the control effectiveness was 80.8% in the F 1 generation and 79.1% in the F 2 generation. The cost of using F 1 sterility to control DBM in a small field was similar to the cost of using pesticides. Therefore, the use of F 1 sterility should be an economically viable control strategy for DBM that also would help protect the environment from the overuse of pesticides. (author)

Insect and Pest Control Newsletter, No. 77, July 2011

International Nuclear Information System (INIS)

2011-07-01

As reported in some previous newsletters, both FAO and IAEA have been undergoing considerable transformation as a result of a major on-going reform process of FAO that started in 2009 and which is scheduled to be fully implemented by 2013. In addition, the IAEA has seen a complete change of senior management and in January 2011 Mr Daud Mohamad was appointed Deputy Director General Nuclear Sciences and Applications and Head of the Department which includes the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. The IAEA has been implementing AIPS, a new IAEA wide Information System for Programme Support, representing a drastic transformation of processes. Until recently there were over 60 different and independent internal information systems and AIPS is replacing most of them with one Oracle product. AIPS also entails the adoption of IPSAS, the International Public Sector Accounting Standards, which is used in a majority of international organizations, involving independentlymaintained standards for financial reporting, considered best practice for organizations like ours. AIPS is being introduced in stages or 'plateaus'. The first plateau is devoted to Finance, Procurement, Transportation and the operational parts of Programme and Project Management. This went live in January 2011, in tandem with our adoption of IPSAS. Plateau 2 is scheduled for 2012. In terms of new publications, a special issue of Genetica on 'Molecular Technologies to Improve the Effectiveness of the Sterile Insect Technique' was recently published. A second publication, 'Rearing Codling Moth for the Sterile Insect Technique' is a text book that was published under the FAO Plant Production and Protection Paper series.

Insect and Pest Control Newsletter, No. 77, July 2011

Energy Technology Data Exchange (ETDEWEB)

NONE

2011-07-15

As reported in some previous newsletters, both FAO and IAEA have been undergoing considerable transformation as a result of a major on-going reform process of FAO that started in 2009 and which is scheduled to be fully implemented by 2013. In addition, the IAEA has seen a complete change of senior management and in January 2011 Mr Daud Mohamad was appointed Deputy Director General Nuclear Sciences and Applications and Head of the Department which includes the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. The IAEA has been implementing AIPS, a new IAEA wide Information System for Programme Support, representing a drastic transformation of processes. Until recently there were over 60 different and independent internal information systems and AIPS is replacing most of them with one Oracle product. AIPS also entails the adoption of IPSAS, the International Public Sector Accounting Standards, which is used in a majority of international organizations, involving independentlymaintained standards for financial reporting, considered best practice for organizations like ours. AIPS is being introduced in stages or 'plateaus'. The first plateau is devoted to Finance, Procurement, Transportation and the operational parts of Programme and Project Management. This went live in January 2011, in tandem with our adoption of IPSAS. Plateau 2 is scheduled for 2012. In terms of new publications, a special issue of Genetica on 'Molecular Technologies to Improve the Effectiveness of the Sterile Insect Technique' was recently published. A second publication, 'Rearing Codling Moth for the Sterile Insect Technique' is a text book that was published under the FAO Plant Production and Protection Paper series.

Inherited sterility in cabbage pest crocidolomia binotalis zell as induced by gamma rays

International Nuclear Information System (INIS)

Sutrisno, S.

1983-01-01

Male moths of crocidolomia binotalis which have been irradiated with gamma rays at a dose of 0.325 kGy were mated with the untreated females. The resulted sterility observed from this mating was 70.86%. A higher sterility was obtained from either male or female F1 offspring when mated with untreated insects (88.62% and 89.46%, respectively). Irradiation of female moths at a dose of 0.15 kGy resulted in 79.6% sterility, however, the sterility of their first offsprings was lower. (author)

Sterilization techniques without heating (ultraviolet ray, radiation and ozone)

International Nuclear Information System (INIS)

Ito, Hitoshi

1991-01-01

The recent demand of consumers for processed foods is characterized by the intention for health and nature, besides, the demand for low sweetness, salt reduction, no additive and freshness becomes strong. In view of the control of microorganisms in products, all these become the negative factors. Accordingly, in order to overcome them, it is urgently desired to develop new technology or to improve conventional methods. As to heating sterilization, the uniform temperature treatment to the inside of foods is difficult, and it cannot be applied to perishables. The high temperature sterilization above 120degC causes the change in nutrition composition and physical properties. Ultraviolet ray and ozone can be used for the sterilization of food surface and powder and liquid foods. Radiation treatment can be applied to packed foods and frozen foods as well as food surface. The principle and the fields of application of ultraviolet ray sterilization, radiation sterilization and ozone sterilization are reported. In the mechanism of these methods, the action to DNA and oxidation are common. (K.I.)

Studies of the ecology of insects sterilized artificially (gamma radiation), 10

International Nuclear Information System (INIS)

Kiyoku, Masao

1976-01-01

Progenies of a combination, T#male# x T#female# (a dose of 2.5 KR from 137 Cs), showed a striking inherited sterility until the fourth generation when the mating was made between inbred lines of the combination. In combinations that irradiated flies were crossed as either male or female with unirradiated ones, some inherited sterility was found in the first and second generations, and was almost entirely disappeared after the third of fourth generation. However, conspicuous inherited sterility was maintained through thirteen generations when progeny flies from the combination of T#male# x U#female#, which was irradiated by a dose of 7.5 KR, were inbred. From cytological studies, it is observed that the oriental fruit fly has chromosome number of n=6 both in salivary gland cells and in meiotic division. An amitosis like cell division occured among meiotic cells of irradiated male flies and those of male progenies that had been mated with unirradiated females. Several different types of chromosome aberrations were found frequently in the salivary gland chromosomes of flies showing inherited sterility. By statistical analysis, the relationship between the second power of frequency (X) of the chromosome aberration and hatchability (Y) of eggs laid was showed to be a straight regression equation, Y=40.69 - (X - 18.75)*. The correlation-coefficient (r), -867* was obtained. (auth.)

Mating competitiveness of sterile male Anopheles coluzzii in large cages.

Science.gov (United States)

Maïga, Hamidou; Damiens, David; Niang, Abdoulaye; Sawadogo, Simon P; Fatherhaman, Omnia; Lees, Rosemary S; Roux, Olivier; Dabiré, Roch K; Ouédraogo, Georges A; Tripet, Fréderic; Diabaté, Abdoulaye; Gilles, Jeremie R L

2014-11-26

Understanding the factors that account for male mating competitiveness is critical to the development of the sterile insect technique (SIT). Here, the effects of partial sterilization with 90 Gy of radiation on sexual competitiveness of Anopheles coluzzii allowed to mate in different ratios of sterile to untreated males have been assessed. Moreover, competitiveness was compared between males allowed one versus two days of contact with females. Sterile and untreated males four to six days of age were released in large cages (~1.75 sq m) with females of similar age at the following ratios of sterile males: untreated males: untreated virgin females: 100:100:100, 300:100:100, 500:100:100 (three replicates of each) and left for two days. Competitiveness was determined by assessing the egg hatch rate and the insemination rate, determined by dissecting recaptured females. An additional experiment was conducted with a ratio of 500:100:100 and a mating period of either one or two days. Two controls of 0:100:100 (untreated control) and 100:0:100 (sterile control) were used in each experiment. When males and females consort for two days with different ratios, a significant difference in insemination rate was observed between ratio treatments. The competitiveness index (C) of sterile males compared to controls was 0.53. The number of days of exposure to mates significantly increased the insemination rate, as did the increased number of males present in the untreated: sterile male ratio treatments, but the number of days of exposure did not have any effect on the hatch rate. The comparability of the hatch rates between experiments suggest that An. coluzzii mating competitiveness experiments in large cages could be run for one instead of two days, shortening the required length of the experiment. Sterilized males were half as competitive as untreated males, but an effective release ratio of at least five sterile for one untreated male has the potential to impact the fertility of

Use of rhodamine B to mark the body and seminal fluid of male Aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases.

Science.gov (United States)

Johnson, Brian J; Mitchell, Sara N; Paton, Christopher J; Stevenson, Jessica; Staunton, Kyran M; Snoad, Nigel; Beebe, Nigel; White, Bradley J; Ritchie, Scott A

2017-09-01

Recent interest in male-based sterile insect technique (SIT) and incompatible insect technique (IIT) to control Aedes aegypti and Aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. Previous reports from other insects indicated rhodamine B, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. Here, we report on the adaptation of this technique for male Ae. aegypti to allow for rapid assessment of competitiveness (mating success) during field releases. Marking was achieved by feeding males on 0.1, 0.2, 0.4 or 0.8% rhodamine B (w/v) in 50% honey solutions during free flight. All concentrations produced >95% transfer to females and successful body marking after 4 days of feeding, with 0.4 and 0.8% solutions producing the longest-lasting body marking. Importantly, rhodamine B marking had no effect on male mating competitiveness and proof-of-principle field releases demonstrated successful transfer of marked seminal fluid to females under field conditions and recapture of marked males. These results reveal rhodamine B to be a potentially useful evaluation method for male-based SIT/IIT control strategies as well as a viable body marking technique for male-based mark-release-recapture experiments without the negative side-effects of traditional marking methods. As a standalone method for use in mating competitiveness assays, rhodamine B marking is less expensive than PCR (e.g. paternity analysis) and stable isotope semen labelling methods and less time-consuming than female fertility assays used to assess competitiveness of sterilised males.

Use of rhodamine B to mark the body and seminal fluid of male Aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases.

Directory of Open Access Journals (Sweden)

Brian J Johnson

2017-09-01

Full Text Available Recent interest in male-based sterile insect technique (SIT and incompatible insect technique (IIT to control Aedes aegypti and Aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. Previous reports from other insects indicated rhodamine B, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. Here, we report on the adaptation of this technique for male Ae. aegypti to allow for rapid assessment of competitiveness (mating success during field releases.Marking was achieved by feeding males on 0.1, 0.2, 0.4 or 0.8% rhodamine B (w/v in 50% honey solutions during free flight. All concentrations produced >95% transfer to females and successful body marking after 4 days of feeding, with 0.4 and 0.8% solutions producing the longest-lasting body marking. Importantly, rhodamine B marking had no effect on male mating competitiveness and proof-of-principle field releases demonstrated successful transfer of marked seminal fluid to females under field conditions and recapture of marked males.These results reveal rhodamine B to be a potentially useful evaluation method for male-based SIT/IIT control strategies as well as a viable body marking technique for male-based mark-release-recapture experiments without the negative side-effects of traditional marking methods. As a standalone method for use in mating competitiveness assays, rhodamine B marking is less expensive than PCR (e.g. paternity analysis and stable isotope semen labelling methods and less time-consuming than female fertility assays used to assess competitiveness of sterilised males.

Prospects of radiation sterilization of medical devices

International Nuclear Information System (INIS)

Hosobuchi, Kazunari

1992-01-01

Since radiation sterilization was first introduced in the United States in 1956 in the field of disposable medical devices, it has become an indispensable technique for sterilization because of the following reasons: (1) introduction into dialyzers, (2) introduction in medical device makers, (3) development of disposable medical devices associated with developing both high molecular chemistry and cool sterilization, (4) rationality of sterilization process, and (5) problems of sterilization with ethylene oxide gas. To promote the further development of radiation sterilization, the following items are considered necessary: (1) an increase in the number of facilities for radiation sterilization, (2) recommendation of the international standardization of sterilization method, (3) decrease in radiation doses associated with sterilization, (4) development of electron accelerators and bremsstrahlung equipments for radiation sources, and (5) simplification of sterilization process management. Factors precluding the development of radiation sterilization are: (1) development of other methods than radiation sterilization, (2) development of technique for sterile products, (3) high facility cost, (4) high irradiation cost, (5) benefits and limits of sterilization markets, and (6) influences of materials. (N.K.)

Electron beam sterilization and composting of sludge and its utilization as fertilizer for greenbelt and farmland

International Nuclear Information System (INIS)

Machi, Sueo; Hashimoto, Shoji

1988-01-01

Sludge should be sterilized to exterminate bacteria prior to its application as a fertilizer in greenbelts or farmland. Furthermore, sludge should be converted into compost to prevent odors or breeding of harmful insects. A technique is developed for complete sterilization of sludge and rapid production of compost. Sludge is first sterilized by electron beams and composting is performed under optimum conditions including the fermentation temperature. Typically, about 10 8 - 10 9 bacteria are contained in 1 g of sludge, with coliforms accounting for about 10 percent of the total bacteria. Irradiation of 15 kGy can reduce the total number of bacteria by 6 - 7 orders of magnitude. Irradiation of 2 kGy can almost completely exterminate coliforms, which are highly sensitive to radiations. This indicates that 0.2-second irradiation is sufficient if a dose rate of 10 kGy/sec is used. After the sterilization process, sludge is composted under the following conditions: temperature of 40 - 50 deg C, initial pH of 7 - 8 and particle size of 5 mm or less. Compared with conventional processes, the maximum fermentation rate is greater by 10 times and can be reached 10 times more rapidly. Conventional processes require more than 10 days while the present technique takes only 2 - 3 days until carbon dioxide stops generating. (Nogami, K.)

The effect of gamma radiation on sterility and mating ability of brown planthopper, Nilaparvata lugens(Stål) in field cage

Science.gov (United States)

Limohpasmanee, W.; Kongratarpon, T.; Tannarin, T.

2017-06-01

The brown planthopper, Nilaparvata lugens(Stål) is the major rice pest in Thailand. Adults and nymphs suck the sap from the rice plant causing it to wilt and transmitting the grassy stunt and the ragged stunt diseases. The population suppression by the sterile insect technique is overwhelmingly a function of mating between sterile males and wild females. The objectives of these experiments were to determine the suitable dose which induces partially sterile in N. lugens and their effect on wild population in the field cages. One-day-old 4th and 5th instar nymphs and adults were irradiated in a 60Co irradiator at the doses of 30, 60, 90 and 120 Gy. It was found that irradiation at the dose of 90 Gy induced complete sterility in female and 78.47 % sterility in males. The inherited sterility were transferred to their progenies and induced 51.46 and 77.00 % sterility in F-1 males and females. The irradiation as the mention dose did not affect mating ability. The competitiveness index was increased when the ratio of irradiated males per normal male was increased. The releasing irradiated males at 10 fold of normal males in field cages could suppress F-1 population 80.11 % and suppress F-2 population 80.32 % when compare with the control. This technique may be applied to delay and/or reduce seasonal increase of brown planthopper.

Search for new amounts of Irrdiation in order to improve the efficacy of the steril insect technique in the control of medfly in Tunisia

International Nuclear Information System (INIS)

Ben Ibrahim, Hatem; Ben Abdallah, Ali

2006-01-01

This survey has for objective to look for a dose of irradiation appropriate and susceptible to contribute to the improvement of the quality of the males radiated in order to make currently moe efficient the sterile bug technique associated in a national program of controlled struggle against the ceratite. For that to make, eight doses of irradiations (60, 70, 80, 90, 100, 110, 120 and 130Gy) have been tried in comparison with a non exposed witness and a witness treated by the dose currently used in the national center of nuclear sciences and technology (145Gy). The quality control of the sterile males has been made in return for tests of emergence, of faculty to the flight, barrenness, longevity and wandering. The last test has been valued after releasing males radiated in orchards of citrus fruits in the region of Hammamet. This work allowed us to clear a dose of 110GY has to improve the quality of the exposed males even and that would be released. This dose permitted to get the best rates of emergence (about 82%), of faculty to the flight (76%), of longevity (65% (about 35% of flies died after 12 hours of observation)) and distribution (8 flies by trap) without affecting the barrenness (about 97% that are considered in the norms of the AIEA). (author). 12 refs

Determination of the dose of gamma radiation sterilization for assessment of biological parameters of male Ceratitis capitada (Diptera: Tephritidae), tsl - Vienna 8 strain

International Nuclear Information System (INIS)

Rocha, Aline Cristina Pereira da

2011-01-01

The Vienna-8, tsl (temperature sensitive lethal) strain of Ceratitis capitata, by presenting mutations that facilitate the mass rearing and release only of sterile males in the field, has been used in (Sterile Insect Technique) programmes. The objective of this study was to determine the radiation dose that provides the highest level of sterility for Vienna-8, tsl males assessing their biological parameters that indicate the quality of sterile males to be released. Brown pupae (males) of the tsl strain were obtained from the mass rearing of the Food Irradiation and Radio entomology laboratory of CENA/USP, and they were irradiated (with gamma radiation - 60 Co) 24 hours before the emergence at rates of 0, 30, 60, 90 and 120 Gy. The determination of the sterilizing dose was based on fertility of sexually mature females of the bisexual strain and not irradiated, mated with males of different treatments. Eggs were collected daily during 6 days, were counted and it was possible to estimate fecundity, and assess the hatching rate. The emergence and flight ability were determined by following the protocol of quality control manual for FAO/IAEA/USDA (2003). To assess the longevity under nutritional stress, the insects were kept a period of 48 h after emergence in the absence of water and food, and after this period, mortality was recorded. The size of the testes (left and right) was obtained by dissecting irradiated and non-irradiated males at the eighth day of life, and measure the testes in an ocular micrometer, considering the maximum length and width of each sample. To determine the sperm number was necessary to dissect the males and break their testicles. No difference was observed in emergence rate, flight ability and longevity of irradiated and non-irradiated males, nor in the fecundity of females mated with males of different treatments. The sterilizing dose that resulted in lower fertility of females was 120 Gy, with 1.5% hatching. Considering the parameters of

Biological Studies On The Radioprotective Role Of Melatonin On The Medfly Ceratitis Capitata (WIED.), Sterile Males

International Nuclear Information System (INIS)

SHOMAN, A.A.; MAHMOUD, E.A.

2010-01-01

The sterile insect technique (SIT) has been used successfully against the medfly. The use of gamma irradiation for sterilizing insects has some adverse effects on their competitiveness which in turn reduces the efficiency of the technique. This study was carried out to evaluate the radioprotective effects of melatonin (N-acetyl-5-methoxytyrptamine) on egg hatchability, larval duration, pupal recovery, pupal weight, adult recovery, sex ratio, adult survival, flight ability and mating competitiveness of the medfly, Ceratitis capitata (Wied.), sterile males against the oxidative stress induced by gamma radiation. The results showed that the use of melatonin before the application of radiation doses did not affect the percentages of egg hatchability, larval duration and pupal weight while the addition of melatonin to the larval diet led to a significant increase in the percent of the pupal recovery as compared with the control group. The results also showed significant increases in adult recovery, adult survival and flight ability of males reared on larval diet containing melatonin before irradiation as compared to those treated with radiation alone. The competitiveness values were drastically decreased (0.30, 0.17 and 0.19) with the increase of gamma irradiation (90, 100 and 110 Gy, respectively). However, the results showed that the competitiveness values were increased in males previously reared on a larval diet containing 1 g melatonin/kg larval media (0.1%) and the produced full grown pupae were irradiated with 90, 100 and 110 Gy. The highest male mating competitiveness was recorded when melatonin was added to the larval diet then irradiated the produced pupae with the sterilizing dose (90 Gy). The results showed that the competitiveness values were 0.67, 0.47 and 0.46 for the doses 90, 100 and 110 Gy, respectively. The results refer to availability of using melatonin as a simple and effective means to improve the quality and the male mating competitiveness of the

Insect Pest Control Newsletter, No. 82, January 2014

International Nuclear Information System (INIS)

2014-01-01

Microbes have been the dominating forms of life, almost since the birth of our planet about 4.5 billion years ago. Being masters of chemical reactions, they regulate the recycling of all major chemicals relevant to life; manage energy sources and the production of fuels; determine the aerobic conditions of our atmosphere and influence our climate; are the catalytic factors of soil fertility, thus affecting agricultural production; and have also been of paramount importance for the health of ecosystems and of all living organisms including humans. Last, but not least, they have been the driving force of the on-going 'biotechnological revolution', which promises to produce more and healthier food, drugs and 'green' fuels. Because of all their unique metabolic properties, microbes have been driving the evolution of life on earth, either by being free-living or by establishing symbiotic associations with diverse organisms including insects. Insects are the most abundant and species-rich animal group on earth, occupying most available ecological niches. Conservative estimates suggest that about 85% of all described animal species are insects; estimates range between 2-30 million insect species and about 10 quintillion (1018) individual insects being alive at any given time (http://www.si.edu/Encyclopedia_SI/nmnh/ buginfo/bugnos.htm). During recent years it has become evident that the ecological and evolutionarily success of insects greatly depends on the sophisticated symbiotic associations they have established with diverse microorganisms, which influence all aspects of their biology, physiology, ecology and evolution. The few examples presented below aim to underline the importance of these symbiotic associations and indicate that the characterization, exploitation and management of insect-bacterial symbiotic associations can significantly contribute to the support and enhancement of sterile insect technique (SIT) programmes against agricultural pests and disease

Insect Pest Control Newsletter, No. 82, January 2014

Energy Technology Data Exchange (ETDEWEB)

NONE

2014-01-15

Microbes have been the dominating forms of life, almost since the birth of our planet about 4.5 billion years ago. Being masters of chemical reactions, they regulate the recycling of all major chemicals relevant to life; manage energy sources and the production of fuels; determine the aerobic conditions of our atmosphere and influence our climate; are the catalytic factors of soil fertility, thus affecting agricultural production; and have also been of paramount importance for the health of ecosystems and of all living organisms including humans. Last, but not least, they have been the driving force of the on-going 'biotechnological revolution', which promises to produce more and healthier food, drugs and 'green' fuels. Because of all their unique metabolic properties, microbes have been driving the evolution of life on earth, either by being free-living or by establishing symbiotic associations with diverse organisms including insects. Insects are the most abundant and species-rich animal group on earth, occupying most available ecological niches. Conservative estimates suggest that about 85% of all described animal species are insects; estimates range between 2-30 million insect species and about 10 quintillion (1018) individual insects being alive at any given time (http://www.si.edu/Encyclopedia{sub S}I/nmnh/ buginfo/bugnos.htm). During recent years it has become evident that the ecological and evolutionarily success of insects greatly depends on the sophisticated symbiotic associations they have established with diverse microorganisms, which influence all aspects of their biology, physiology, ecology and evolution. The few examples presented below aim to underline the importance of these symbiotic associations and indicate that the characterization, exploitation and management of insect-bacterial symbiotic associations can significantly contribute to the support and enhancement of sterile insect technique (SIT) programmes against agricultural pests and disease

Vasectomy occlusion techniques for male sterilization.

Science.gov (United States)

Cook, L A; Vliet, H; Pun, A; Gallo, M F

2004-01-01

Vasectomy is an increasingly popular and effective family planning method. A variety of vasectomy techniques are used worldwide including various vas occlusion techniques (excision and ligation, thermal or electrocautery, and mechanical and chemical occlusion methods), vas irrigation and fascial interposition. Vasectomy guidelines largely rely on information from observational studies. Ideally, the choice of vasectomy techniques should be based on the best available evidence from randomized controlled trials. The objective of this review was to compare the effectiveness, safety, acceptability and costs of vasectomy techniques for male sterilization. We searched the computerized databases the Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, EMBASE, Popline and LILACS. In addition, we searched the reference lists of relevant articles and book chapters. We included randomized controlled trials and controlled clinical trials comparing vasectomy techniques. We assessed all titles and abstracts located in the literature searches and two reviewers independently extracted articles identified for inclusion. Data were presented in the text of the review. Outcome measures include contraceptive efficacy, safety, discontinuation, and acceptability. Two trials compared vas occlusion with clips versus a conventional vasectomy technique; both were of poor quality. Neither trial found a difference between the two groups with regard to the primary outcome of failure to reach azoospermia. Four trials examined vas irrigation: three compared water irrigation with no irrigation and one compared water irrigation with euflavine. All of the trials were of poor quality. None of the trials found a significant difference between the groups with respect to the primary outcome of time to azoospermia. However, one trial found that the median number of ejaculations to azoospermia was significantly lower in the euflavine group compared to the water irrigation group. The one trial

Genetic and Cytogenetic Basis of Radiation-Induced Sterility in the Adult Male Cabbage Looper Trichoplusia Ni

Energy Technology Data Exchange (ETDEWEB)

North, D. T.; Holt, G. G. [Metabolism and Radiation Research Laboratory, Entomology Research Division, ARS, United States Department of Agriculture, Fargo, ND (United States)

1968-06-15

The relationship of egg hatch as a function of radiation dose is of a two-hit nature when irradiated adult males are mated to non-irradiated virgin females. Sterility as a function of dose is usually linear in insects. However, other species of moths respond similarly to irradiation, so it is indicative that the mechanism involved in producing sterility in Lepidoptera is basically different from that in other insects. The significance of the two-hit kinetics is discussed in relation to the chromosome structure and possible mechanisms for the induction of sterility in Lepidoptera. Many workers using Lepidoptera have reported that females mated to irradiated males oviposit substantially fewer eggs than normally. This response has been correlated to a lack of sperm transfer by irradiated males, even though they pass a spermatophore. The phenomenon is dose-dependent. Although Lepidoptera are far more radioresistant than other insect species when measured by the induction of sterility in the male, there appears to be very little difference when longevity is used as the criterion. The radiation dose required to reduce the lifespan of a newly emerged cabbage looper male by 50% was found to be approximately the same as that for the house fly. High doses of radiation have no immediate effect on the mating behaviour of the irradiated male. With a recessive eye-colour mutant as a sperm marker, it was determined in tests utilizing double matings that the second mating is the effective mating. Sperm mixing is not prevalent; rather it appears to be a 'sperm flushing' phenomenon in that sperm from the first mating are displaced by sperm from the second mating. Radiation studies with the cabbage looper have demonstrated that the progeny of a cross where the male parent receives a sub-sterilizing dose of gamma radiation are often semi-sterile when mated to non-irradiated individuals. The amount of inherited sterility is directly dependent on the amount of radiation given the

Nuclear techniques in food production

International Nuclear Information System (INIS)

Merlin, J.P.C.

1975-01-01

This study is divided into three parts. The first, devoted to the use of radiations in food production, deals especially with artificial mutagenesis, selectors taking advantage of altered hereditary features in plants from irradiated seed; sterilization of animals to eliminate harmful insects (male sterilization technique); the lethal power of radiations used for the production of animal vaccins, attenuated by irradiation, against organisms which infest or degrade food products. Part two shows that radioactive atoms used as tracers to reveal migrations and chemical transformations of products such as fertilizers and pesticides can speed up all kinds of agronomical research. Their possibilities in research on animal feeding and to detect poisonous substances in foodstuffs are also mentioned. The last part is devoted to the use of nuclear techniques in irrigation and more precisely in the study of underground water flows soil moisture and lastly the future of nuclear desalination [fr

Utilization of organic residues using heterotrophic microalgae and insects.

Science.gov (United States)

Pleissner, Daniel; Rumpold, Birgit A

2018-02-01

Various organic residues occur globally in the form of straw, wood, green biomass, food waste, feces, manure etc. Other utilization strategies apart from anaerobic digestion, composting and incineration are needed to make use of the whole potential of organic residues as sources of various value added compounds. This review compares the cultivation of heterotrophic microalgae and insects using organic residues as nutrient sources and illuminates their potential with regard to biomass production, productivity and yield, and utilization strategies of produced biomasses. Furthermore, cultivation processes as well as advantages and disadvantages of utilization processes are identified and discussed. It was shown that both heterotrophic algae and insects are able to reduce a sufficient amount of organic residues by converting it into biomass. The biomass composition of both organisms is similar which allows similar utilization strategies in food and feed, chemicals and materials productions. Even though insect is the more complex organism, biomass production can be carried out using simple equipment without sterilization and hydrolysis of organic residues. Contrarily, heterotrophic microalgae require a pretreatment of organic residues in form of sterilization and in most cases hydrolysis. Interestingly, the volumetric productivity of insect biomass exceeds the productivity of algal biomass. Despite legal restrictions, it is expected that microalgae and insects will find application as alternative food and feed sources in the future. Copyright © 2017 Elsevier Ltd. All rights reserved.

Induction of inherited sterility and sex ratio distribution due to exposure to substerilising doses of gamma radiation in cotton bollworm Earias vittella fabricius

International Nuclear Information System (INIS)

Tamhankar, A.J.; Shantharam, K.

2005-01-01

Substerilising doses of gamma radiation induced inherited sterility and sex ratio distortion in the cotton bollworm Earias vittella fabricius. Adults irradiated with 75 Gy and self-crossed, provided sterile F 1 adults, suitable for direct use in sterile insect technique (SIT). In case of 50 Gy, the F 1 adults, when backcrossed, produced F 2 progeny with sex ratio in favour of females (1: >3). With 25 Gy, a sex ratio distortion was recorded in F 1 (1 male: 2.25 females) and self-crossing of F 1 resulted in progeny with a sex ratio of 3:1. Backcrossing of the F 1 female produced F 2 progeny with a sex ratio of 1:5. These results have implications in improving cost/benefit ratio of SIT for this species. (author)

Preliminary studies on inherited sterility for field management of diamondback moth (plutella xylostella) (Lepidoptera: plutellidae) on crucifiers in Ghana

International Nuclear Information System (INIS)

Ewusie, E.A.; Nkumsah, A.K.; Alimatu, S.; Osae, M.Y.

2010-01-01

The diamondback moth (DBM), plutella xylostella (L.) (Lepidoptera: plutellidae) is the most destructive insect pest crucifiers in Ghana and the world over. It is estimated to cost about USD 1 billion to control annually worldwide. Reliance on chemicals as the sole control measure for diamondback moth has resulted in the development of a myriad of problems including resistance, high residue levels on produce, destruction of natural enemies and pest resurgence among others. Inherited sterility in Lepidoptera insects has a potential for suppressing DBM populations. We conducted this study to evaluate the use of the technique to manage the diamond back moth in Ghana. When 3 - 4 day old pupae were treated with 130 Gy and 150 Gy of gamma radiation, 47% and 46% respectively of the male pupae developed as normal adults while 40% and 17% respectively of the female pupae developed as normal adults. However, radiation-induced reductions in fecundity and egg viability were expressed in the parental and first filial (F 1 ) generations. Sterility exceeded 66% in the treated parental male and 92% in the treated parental female in both treatments as compared with 78% and 95% in treated F 1 male and female, respectively. The sex ratio was skewed in favour of males in the parental progeny. These results indicate the possibility of using inherited sterility for DBM control. (au)

Plasma Sterilization: New Epoch in Medical Textiles

Science.gov (United States)

Senthilkumar, P.; Arun, N.; Vigneswaran, C.

2015-04-01

Clothing is perceived to be second skin to the human body since it is in close contact with the human skin most of the times. In hospitals, use of textile materials in different forms and sterilization of these materials is an essential requirement for preventing spread of germs. The need for appropriate disinfection and sterilization techniques is of paramount importance. There has been a continuous demand for novel sterilization techniques appropriate for use on various textile materials as the existing sterilization techniques suffer from various technical and economical drawbacks. Plasma sterilization is the alternative method, which is friendlier and more effective on the wide spectrum of prokaryotic and eukaryotic microorganisms. Basically, the main inactivation factors for cells exposed to plasma are heat, UV radiation and various reactive species. Plasma exposure can kill micro-organisms on a surface in addition to removing adsorbed monolayer of surface contaminants. Advantages of plasma surface treatment are removal of contaminants from the surface, change in the surface energy and sterilization of the surface. Plasma sterilization aims to kill and/or remove all micro-organisms which may cause infection of humans or animals, or which can cause spoilage of foods or other goods. This review paper emphasizes necessity for sterilization, essentials of sterilization, mechanism of plasma sterilization and the parameters influencing it.

Comparison of Techniques for Sampling Adult Necrophilous Insects From Pig Carcasses.

Science.gov (United States)

Cruise, Angela; Hatano, Eduardo; Watson, David W; Schal, Coby

2018-02-06

Studies of the pre-colonization interval and mechanisms driving necrophilous insect ecological succession depend on effective sampling of adult insects and knowledge of their diel and successional activity patterns. The number of insects trapped, their diversity, and diel periodicity were compared with four sampling methods on neonate pigs. Sampling method, time of day and decomposition age of the pigs significantly affected the number of insects sampled from pigs. We also found significant interactions of sampling method and decomposition day, time of sampling and decomposition day. No single method was superior to the other methods during all three decomposition days. Sampling times after noon yielded the largest samples during the first 2 d of decomposition. On day 3 of decomposition however, all sampling times were equally effective. Therefore, to maximize insect collections from neonate pigs, the method used to sample must vary by decomposition day. The suction trap collected the most species-rich samples, but sticky trap samples were the most diverse, when both species richness and evenness were factored into a Shannon diversity index. Repeated sampling during the noon to 18:00 hours period was most effective to obtain the maximum diversity of trapped insects. The integration of multiple sampling techniques would most effectively sample the necrophilous insect community. However, because all four tested methods were deficient at sampling beetle species, future work should focus on optimizing the most promising methods, alone or in combinations, and incorporate hand-collections of beetles. © The Author(s) 2018. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Application of nuclear techniques in improving agricultural productivity with particular reference to pasture management

International Nuclear Information System (INIS)

Majid Ajorlo; Ramdzani Abdullah

2009-01-01

Nuclear techniques are appropriate tools to settle problems for agriculture or natural resources which cannot be solved using conventional agricultural techniques. In this paper, the research activities carried out and the achievements so far obtained in the agricultural specialties -all by using nuclear techniques- with particular reference to pasture management are discussed. Insect pest control by Sterile Insect Techniques (SIT); soil and water management using neutron moisture probes; determining N-fixation capacity of legumes using N-15 isotope and nuclear methods; soil erosion measurement using of fallout radionuclide such as Cs-137; monitoring the movement of saline water in salt-affected land using isotopes of chlorine; livestock production and health by RIA and ELISA are some of achievements so far obtained in improving agricultural productivity. The ultimate goal of the nuclear technique investigation in agriculture is to use the resources efficiently in obtaining higher plant and livestock yields while increasing the quality and protecting them against insects, diseases and weeds. (Author)

Insect and Pest Control Newsletter, No. 88, January 2017

International Nuclear Information System (INIS)

2017-01-01

In our NL 84, we reported on the ground-breaking for the ReNuAL project (Renovation of the Nuclear Applications Laboratories), which includes the FAO/IAEA Agriculture & Biotechnology Laboratories. The laboratories are unique within the United Nations system in providing Member States with direct access to scientific training, technology and analytical services. ReNuAL is getting under way with the construction of a new Insect Pest Control Laboratory (IPCL), pictured on the previous page, due for completion by the end of 2017. In 2016, we also reported on the increasing demands from our FAO and IAEA Member States to expand our focus from developing and transferring the SIT for major crop and livestock insect pests to major disease-transmitting mosquitoes. Looking to the year ahead, we are organizing the Third FAO/IAEA International Conference on Area-wide Management of Insect Pests: Integrating the Sterile Insect and Related Nuclear and Other Techniques, at the IAEA Headquarters in Vienna, Austria, from 22–26 May 2017. The programme that is being prepared looks very promising and will cover relevant current scientific and applied topics. A number of prominent speakers have been invited to debate new developments and trends. We expect around 400 scientists from all continents and look forward to a successful conference and your active participation.

Induction of sterility in Anastrepha Fraterculus (Diptera: Tephritidae) by gamma radiation

International Nuclear Information System (INIS)

Allinghi, A.; Gramajo, C.; Willink, E.; Vilardi, J.

2007-01-01

In relation to the application of the sterile insect technique (SIT) for the South American fruit fly Anastrepha fraterculus (Wiedemann), we analyzed the effect on adult fertility of different doses of gamma irradiation and the age of pupae at the time of irradiation. In a first experiment, we applied doses of 50, 70, and 90 Gy to pupae at 24, 48, 72, and 96 h before adult emergence. In a second experiment we irradiated pupae 48 h before emergence with 20, 40, and 60 Gy and estimated male and female fertility and sperm transfer by irradiated males. The results indicated pupal age at irradiation does not significantly affect male fertility. If males irradiated with 60 Gy are crossed to non-irradiated females the fertility is about 1%. Females irradiated with 40 Gy did not lay eggs independently of the male to which they mated. No significant effects of radiation were observed with respect to the ability of males to transfer sperm. A dose of 70 Gy applied 48 h before adult emergence induces 100% sterility in both males and females. (author) [es

Incorporation of diapause into codling moth mass rearing: Production advantages and insect quality issues

International Nuclear Information System (INIS)

Bloem, Stephanie; Calkins, Carrol O.; Bloem, Ken A.

2000-01-01

The codling moth (CM), Cydia pomonella L. (Lepidoptera: Tortricidae) is a widely distributed species and the key pest of apples and pears in orchards of the Pacific Northwest. CM possesses facultative diapause and the mature 5th instar larva is the overwintering stage (Brown 1991). British Columbia, Canada, is attempting to eradicate CM using the sterile insect technique (SIT). The Okanagan-Kootenay Sterile Insect Release (SIR) Program began releasing sterile CM in 1994 (Dyck et al. 1993, Bloem and Bloem 1996) and results in recent years have been excellent. Despite the fact that standard rearing operations have gone extremely well, and weekly CM production has increased from 8.7 million CM per week in 1994 to 14.2 million per week in 1997, the ability to mass rear diapausing CM holds a number of advantages that are currently not available. The ability to stockpile CM larvae in diapause throughout the fall and winter months when the mass rearing facility is underutilised would: 1) provide additional CM for release during the spring/summer field season, 2) provide back-up material to guard against colony losses due to operational failures and/or pathogen outbreaks, 3) allow for a potentially more cost-effective use of the facility by maintaining production year round, 4) facilitate the development of mass rearing techniques for key parasitoids that attack the overwintering stage, 5) allow for the easy shipment (and sale) of CM to researchers around the world. Here we outline the current standard CM mass rearing procedures and discuss research that has allowed diapausing CM to be mass reared (Bloem et al. 1997). We also discuss research that suggests diapaused CM are more competitive than standard non-diapaused CM (Bloem et al. 1998)

Development of female medfly attractant systems for trapping and sterility assessment. Proceedings of a final research co-ordination meeting

International Nuclear Information System (INIS)

1999-07-01

Practical application of the sterile insect technique (SIT) against major insect pests will continue to increase as the repeated use of insecticides is recognized as an environmental problem. In the case of the Mediterranean fruit fly (medfly), which attacks over 300 species of fruits and vegetables in tropical, subtropical, and temperate climates on all five continents, control is still largely based on frequent insecticide spraying, often more than 10 sprays per fruiting season. Methods for population estimation, which accurately reflect changes due to movement, mortality or reproduction, are a prerequisite for effective pest management and in particular for use of SIT. With better monitoring tools medfly populations can be estimated more accurately and compared under different conditions, to guide decisions on alternative (i.e. more effective and more environment-friendly) control strategies. In support of this need in the application of SIT field programmes against medfly, an FAO/IAEA co-ordinated research project (CRP) was carried out which resulted in a TECDOC published in 1996 on Standardization of Medfly Trapping for Use in sterile insect technique Programmes. Following the development of male only genetic sexing strains at the FAO/IAEA Agriculture and Biotechnology Laboratories in Seibersdorf, Austria, it was recognized that the development of a female medfly targeted trapping system, in conjunction with only male sterile releases, would improve the efficacy of the SIT, reduce costs, and more effectively utilize sterile males. As a result, a new FAO/IAEA Co-ordinated Research Project on Development of Female Medfly Attractant Systems for Trapping and Sterility Assessment was initiated in 1994 with the objective to develop new synthetic female medfly attractants and to determine their efficacy compared to proteinaceous baits under different weather, host-tree and population density conditions. Findings obtained during the course of this 5-year CRP are

Development of female medfly attractant systems for trapping and sterility assessment. Proceedings of a final research co-ordination meeting

Energy Technology Data Exchange (ETDEWEB)

NONE

1999-07-01

Practical application of the sterile insect technique (SIT) against major insect pests will continue to increase as the repeated use of insecticides is recognized as an environmental problem. In the case of the Mediterranean fruit fly (medfly), which attacks over 300 species of fruits and vegetables in tropical, subtropical, and temperate climates on all five continents, control is still largely based on frequent insecticide spraying, often more than 10 sprays per fruiting season. Methods for population estimation, which accurately reflect changes due to movement, mortality or reproduction, are a prerequisite for effective pest management and in particular for use of SIT. With better monitoring tools medfly populations can be estimated more accurately and compared under different conditions, to guide decisions on alternative (i.e. more effective and more environment-friendly) control strategies. In support of this need in the application of SIT field programmes against medfly, an FAO/IAEA co-ordinated research project (CRP) was carried out which resulted in a TECDOC published in 1996 on Standardization of Medfly Trapping for Use in sterile insect technique Programmes. Following the development of male only genetic sexing strains at the FAO/IAEA Agriculture and Biotechnology Laboratories in Seibersdorf, Austria, it was recognized that the development of a female medfly targeted trapping system, in conjunction with only male sterile releases, would improve the efficacy of the SIT, reduce costs, and more effectively utilize sterile males. As a result, a new FAO/IAEA Co-ordinated Research Project on Development of Female Medfly Attractant Systems for Trapping and Sterility Assessment was initiated in 1994 with the objective to develop new synthetic female medfly attractants and to determine their efficacy compared to proteinaceous baits under different weather, host-tree and population density conditions. Findings obtained during the course of this 5-year CRP are

Radiosterilization and gas sterilization

International Nuclear Information System (INIS)

Schultz, H.A.; Gruenewald, T.

1987-01-01

The book presents a survey of the principles of cold sterilization by radiation or gas, and also of the problems involved. The reader who has to do with sterilization of some kind or other, as e.g. sterilization of medicaments, medical supplies, medical device, drugs, grafts, or food, will find comprehensive information on the various available methods and their effects, as well as on monitoring procedures and techniques, together with proposals for efficient documentation and validation of partial sterilization processes. The introduction summarizes the potentials of sterilization by radiation, and the subsequent by radiation, and the subsequent chapters explain the specific applicabilities of radiosterilization along with the most important criteria for evaluating the sterilizing efficiency. The information on the legal situation and requirements takes into account the second amendment of the Medical Preparations Act. With 38 figs., 14 tabs [de

Suppression of the Mediterranean fruit fly in Tunisia with released sterile insects

International Nuclear Information System (INIS)

Cheikh, M.; Howell, J.F.; Harris, E.J.; Salah, H.B.; Soria, F.

1975-01-01

The Government of Tunisia, U. S. Agency for International Development, and U. S. Department of Agriculture cooperatively developed a program for suppression of Ceratitis capitata (Wiedemann) in Tunisia. Mediterranean fruit flies were reared on an artificial diet, sterilized with 10 krad irradiation from a cobalt source, and the emerged adults were marked and then distributed by hand throughout the release area, 600 ha in the vicinity of Porto-Farina. Some aerial releases were made late in the season. Winter larval hosts were removed to lower the overwintering population, and sterile fly releases were begun early (Mar.1) to prevent fertile matings of flies that emerged during warm winter days. All fruit on the periphery of the release area was sprayed periodically (5 applications), as was the major fruit-growing areas within the region but outside the test zone, to minimize the possibility of fertile flies entering the release area. Daily from March to November, ca. 1,000,000 sterile flies were released. Trap catches indicated that the suppression obtained was about equal to that obtained using poison bait sprays. The early preferred host crops had no or negligible infestation (loquats, apricots, early peaches, and figs). The infestation of preferred summer fruits (peaches and figs) was reduced but not controlled. Less susceptible summer fruits were seldom infested. The estimated population was ca. 82.3 percent lower than in the previous year when no releases were made. Isolation and sterile fly distribution was inadequate to completely suppress the Mediterranean fruit fly population. (U.S.)

Mating-induced changes in olfactory-mediated behavior of laboratory-reared normal, sterile, and wild female Mediterranean fruit flies (Diptera: Tephritidae) mated to conspecific males

International Nuclear Information System (INIS)

Jang, E.B.; McInnis, D.O.; Lance, D.R.; Carvalho, L.A.

1998-01-01

Laboratory-reared normal, sterile, and wild female Mediterranean fruit flies, Ceratitis capitata (Wiedemann), were mated with laboratory-reared normal, sterile, and wild male flies to assess the ability of males to alter olfactory-mediated behavioral responses of females to male-produced pheromone or host fruit odor. Virgin females of all 3 types showed a preferential attraction and arrestment on yellow spheres emitting male-produced pheromone in a laboratory flight tunnel. Laboratory-reared normal and wild females mated to laboratory reared normal, sterile, or wild males switched their behavior showing strong preferential attraction to, arrestment on, and egg-laying in (for laboratory-reared females) yellow spheres emitting host fruit odor (guava) over male-produced pheromone. Sterile females did not show a significant switch in behavior except when mated to sterile males. The olfactory-mediated behavioral switch was most evident in the laboratory-reared normal female × laboratory-reared normal male mating. These findings suggest that irradiation of males inducing gamete sterility does not affect the factor(s) from the male accessory gland associated with altering female olfactory behavior. The ability of sterile males to alter adequately olfactory-mediated behavior of wild females is discussed in the context of the sterile insect technique for control of Mediterranean fruit flies in the field

Sterilization of space hardware.

Science.gov (United States)

Pflug, I. J.

1971-01-01

Discussion of various techniques of sterilization of space flight hardware using either destructive heating or the action of chemicals. Factors considered in the dry-heat destruction of microorganisms include the effects of microbial water content, temperature, the physicochemical properties of the microorganism and adjacent support, and nature of the surrounding gas atmosphere. Dry-heat destruction rates of microorganisms on the surface, between mated surface areas, or buried in the solid material of space vehicle hardware are reviewed, along with alternative dry-heat sterilization cycles, thermodynamic considerations, and considerations of final sterilization-process design. Discussed sterilization chemicals include ethylene oxide, formaldehyde, methyl bromide, dimethyl sulfoxide, peracetic acid, and beta-propiolactone.

Report of the Advisory Group Meeting on Genetic Methods of Insect Control

International Nuclear Information System (INIS)

1987-01-01

Despite the availability of a range of modern pest control techniques, insects remain a major cause of production losses in agriculture and contribute significantly to diseases of man and livestock. The increasing incidence of pesticide resistance, and concerns over the environmental impact of residues, have highlighted the need for improved technologies. As a result, genetic methods of pest control, including the use of irradiation sterilized insects, have become of increasing importance. It is therefore essential that the Joint FAO/IAEA Division continues to promote the development and application of this method of pest control. The advisory group concluded that the opportunities for genetic control might be widened by the application of new techniques, particularly recombinant DNA technology. The scope for integration of genetic control methods with other control measures, and ist use as a temporary suppressive measure on an area-wide basis was also recognized. Examples are given from representative groups of insect pests to illustrate how these concepts can be applied. The advisory group regarded the Seibersdorf laboratory as a unique facility for the conduct of tactical research related to mass-rearing and release procedures for major pests such as medfly and tsetse spp. Associated research on genetic sexing of medfly, diet recycling and the development of more environmentally acceptable alternatives for pre-release suppression of medfly were considered to be important research projects. The advisory group concluded that the laboratory should continue to remain a centre of excellence for mass-rearing technologies for medfly and tsetse spp., and for training scientists and technicians from developing countries. The Joint FAO/IAEA Division currently plays a major co-ordinating and supportive role for those areas of international research which impinge on genetic control. The advisory group believes that the Joint FAO/IAEA Division should maintain its initiative

Report of the Advisory Group Meeting on Genetic Methods of Insect Control

Energy Technology Data Exchange (ETDEWEB)

NONE

1987-07-01

Despite the availability of a range of modern pest control techniques, insects remain a major cause of production losses in agriculture and contribute significantly to diseases of man and livestock. The increasing incidence of pesticide resistance, and concerns over the environmental impact of residues, have highlighted the need for improved technologies. As a result, genetic methods of pest control, including the use of irradiation sterilized insects, have become of increasing importance. It is therefore essential that the Joint FAO/IAEA Division continues to promote the development and application of this method of pest control. The advisory group concluded that the opportunities for genetic control might be widened by the application of new techniques, particularly recombinant DNA technology. The scope for integration of genetic control methods with other control measures, and ist use as a temporary suppressive measure on an area-wide basis was also recognized. Examples are given from representative groups of insect pests to illustrate how these concepts can be applied. The advisory group regarded the Seibersdorf laboratory as a unique facility for the conduct of tactical research related to mass-rearing and release procedures for major pests such as medfly and tsetse spp. Associated research on genetic sexing of medfly, diet recycling and the development of more environmentally acceptable alternatives for pre-release suppression of medfly were considered to be important research projects. The advisory group concluded that the laboratory should continue to remain a centre of excellence for mass-rearing technologies for medfly and tsetse spp., and for training scientists and technicians from developing countries. The Joint FAO/IAEA Division currently plays a major co-ordinating and supportive role for those areas of international research which impinge on genetic control. The advisory group believes that the Joint FAO/IAEA Division should maintain its initiative

Insect disinfestation in smoked fish by gamma irradiation

International Nuclear Information System (INIS)

Sudatis, Boonya; Banditsing, Chettachai

1982-01-01

The experiment on insect disinfestation in smoked fish by gamma irradiation was done by irradiating 2-day-old eggs, 28-day-old larvae, 5-day-old pupae, and 5-day-old adults, reared at 26 +- 1 degC and 75-70% RH., with doses of 0-.5, 0-20 Krad respectively for sterility dose and with doses of 0-2, 0-6, 0-60, 20-60 Krad respectively for LD 50 . The results obtained from this experiment are as follows: (1) For the study of all stages of this insect sterility doses, there was no irradiated egg hatch in all doses and only irradiated larvae with 1-5 Krad reached pupal stage but emerged as malformed adults and died. Furthermore, the sterility doses for pupal and adult stage are both at 7.5 Krad. However, the percent of egg hatch from 5 Krad pupae and 5 Krad adults are .18 and .4 respectively. (2) The LD 50 checked at different times after irradiation in each stage for 2-day-old eggs after 2 days, for 28-day-old larvae after 7 days, 5-day-old pupae after 5 days, and for 5-day-old adults after 5 days are .48, 12.85, 18.5 and 29.78 Krad respectively

Performance of medical residents in sterile techniques during central vein catheterization: randomized trial of efficacy of simulation-based training.

Science.gov (United States)

Khouli, Hassan; Jahnes, Katherine; Shapiro, Janet; Rose, Keith; Mathew, Joseph; Gohil, Amit; Han, Qifa; Sotelo, Andre; Jones, James; Aqeel, Adnan; Eden, Edward; Fried, Ethan

2011-01-01

Catheter-related bloodstream infection (CRBSI) is a preventable cause of a potentially lethal ICU infection. The optimal method to teach health-care providers correct sterile techniques during central vein catheterization (CVC) remains unclear. We randomly assigned second- and third-year internal medicine residents trained by a traditional apprenticeship model to simulation-based plus video training or video training alone from December 2007 to January 2008, with a follow-up period to examine CRBSI ending in July 2009. During the follow-up period, a simulation-based training program in sterile techniques during CVC was implemented in the medical ICU (MICU). A surgical ICU (SICU) where no residents received study interventions was used for comparison. The primary outcome measures were median residents' scores in sterile techniques and rates of CRBSI per 1,000 catheter-days. Of the 47 enrolled residents, 24 were randomly assigned to the simulation-based plus video training group and 23 to the video training group. Median baseline scores in both groups were equally poor: 12.5 to 13 (52%-54%) out of maximum score of 24 (P = .95; median difference, 0; 95% CI, 0.2-2.0). After training, median score was significantly higher for the simulation-based plus video training group: 22 (92%) vs 18 (75%) for the video training group (P training in sterile techniques during CVC is superior to traditional training or video training alone and is associated with decreased rate of CRBSI. Simulation-based training in CVC should be routinely used to reduce iatrogenic risk. ClinicalTrials.gov; No.: NCT00612131; URL: clinicaltrials.gov.

Radiation sterilization of the greenhouse white fly (Trialeurodes vaporariorum Westw., Homoptera, Aleyrodidae). Part 2

International Nuclear Information System (INIS)

Genchev, N.

1987-01-01

Results are reported of studies of the sterilizing effect on puparia and imagoes of the greenhouse white fly (T. vaporariorum). The irradiation was made by gamma rays at the rate of 12+-5% rad/s. The parameters of radiation-induced sterility were sex determined. The sterilizing effect was expressed in emergence of dominant lethal mutations in spermatozoa accompanied by partial sperm inactivation and of partial aspermia. The absolute sterilizing doses for the two ontogenetic stage are 6 and 7 krad respectively. Radiation induced sterilization of the male parent resulted in forced arrhenotoky in F 1 and was regarded as a transformation of parthogenesis factor necessary for the normal propagation in the insect process into one that is lethal to the population factor. Radiation induced female sterility in females was expressed in the emergence of dominant lethals in oocytes. The absolute sterilizing doses for the puparium and the imago were 5 and 6 krad, respectively. Doses ≥4 krad caused partial infertility

Early puerperal laparoscopic sterilization--a new technique.

Directory of Open Access Journals (Sweden)

Parulekar S

1985-07-01

Full Text Available This paper describes the results of 23 cases of puerperal laparoscopic sterilization performed within 72 hours of delivery. A new method was employed to avoid injury to the uterine fundus and to make the tubes more accessible. Before insertion of the Veress needle, the anterior and posterior lips of the cervix were held with forceps and Hulka′s uterine manipulator was introduced into the uterine cavity. The uterus was pulled down toward the introitus by applying traction to the sponge-holding forceps. Pneumoperitoneum of 3-4 liters was induced through use of a Veress needle, followed by introduction of a Storz single puncture operative laparoscope through an incision in the lower umbilical fold. Tubal occlusion was achieved with silastic bands. The average time required for sterilization from induction of general anesthesia was 6 minutes. There was no instance of injury to the uterine fundus, nor were there any cases of uterine perforation, injury to other pelvic structures, laceration of the cervix, or puerperal sepsis. At the time of follow-up 15 days after the procedure, all patients were in satisfactory condition. It is concluded that this method can enhance postpartum family planning programs. Since it can be carried out within the 1st 48 hours after delivery, the routine hospital stay of 3 days does not have to be prolonged as a result of sterilization.

Sterilization of the melon fly, Dacus cucurbitae Coquillett (Diptera: Tephritidae), with gamma-radiation: Effect of dose on oviposition behavior of irradiated females

International Nuclear Information System (INIS)

Teruya, T.

1990-01-01

In a laboratory condition, the visiting and the puncturing frequencies of gamma-irradiated Dacus cucurbitae females on cucumber Cucumis sativus fruits were examined. In the non-irradiated females, the frequencies reached equilibrium ca. 1 week after adult emergence. The frequencies of the irradrated females decreased with irradiation dosage, but gradually resumed frequency with age. A similar trend was found in the relationship between the irradiation dose and the rates of the puncturing frequency to the visiting frequency. As the irradiation dose increased, the rate of under-developed ovaries increased. The ratio of cumulative puncturing frequency in the 70 Gy irradiated (completely sterile) females to that of the non-irradiated females was estimated as 1/200 when daily survival rate in the field was assumed to be 0.85. The completely sterile adult females (40 days old) made punctures on all sizes of cucumber cultivated in a greenhouse. However, these punctures do not significantly damage the fruit. The sting of the sterile melon fly would not be a serious problem in eradication programs based on the Sterile Insect Technique

Sterile medfly males of the tsl Vienna 8 genetic sexing strain display improved mating performance with ginger root oil

International Nuclear Information System (INIS)

Paranhos, Beatriz Jordao; Alves, Renata Morelli; McInnis, Donald; Damasceno, Itala; Malavasi, Aldo; Goncalves, Nilmara; Costa, Maria de Lourdes; Walder, Julio; Nascimento, Antonio

2006-01-01

A key point of the sterile insect technique applied to the medfly, Ceratitis capitata, is that the sterile males produced in the laboratory should have at least a minimal sexual compatibility with wild females. Among several genetic sexing tsl (Temperature Sensitive Lethal) strains of C. capitata mass-reared around the world, the Biofabrica Moscamed Brasil has chosen the most recent mass produced tsl strain, Vienna 8 (V8), which has been evaluated in the San Francisco River Valley, Brazil, since April, 2005. The tests were accomplished in field cages, with different treatments for V8 males, sterile or fertile, exposed to the aroma of ginger root oil (GRO) or not, versus wild males and females. Males of one strain (V8 or wild) were painted white on the thorax the day before the mating tests. All the insects were virgin, and early in the morning (7-8 A.M.) males were released inside the field cages, 10 min. before females. Mating pairs were collected in glass vials, until early afternoon. From this raw data, both the type of male mating and the time in copula were recorded for each pair. Then, the total percentage of mated females, the RSI (Relative Sterility Index), and Fried's competitiveness values (C), were calculated for each field cage. The percentage of females mated was statistically higher to sterile males exposed to GRO than to non exposed to GRO. Time in copula was significantly higher for wild flies than for laboratory flies, except for the case of fertile V8 males exposed to GRO x wild females. The RSI and C values were significantly higher for V8 males (irradiated and fertile) treated with GRO than for V8 males not treated with GRO. The results indicate that there is adequate sexual compatibility between sterile males of the tsl Vienna 8 strain and wild C. capitata females from the San Francisco River Valley, Brazil. Also, the radiation dose of 95 Gy, used to sterilize the males, did not affect their sexual activity. Ginger root oil acted as a

Sterile medfly males of the tsl Vienna 8 genetic sexing strain display improved mating performance with ginger root oil

Energy Technology Data Exchange (ETDEWEB)

Paranhos, Beatriz Jordao; Alves, Renata Morelli, E-mail: bjordao@cpatsa.embrapa.b [EMBRAPA Semi-Arido, Petrolina, PE (Brazil); McInnis, Donald [U.S. Department of Agriculture (USDA/ARS/PBARC), Honolulu, HI (United States). Agricultural Research Service. Pacific Basin Agricultural Research Center; Uramoto, Keiko [Universidade de Sao Paulo (USP), SP (Brazil); Damasceno, Itala; Malavasi, Aldo [Biofabrica Moscamed Brasil, Juazeiro, BA (Brazil); Goncalves, Nilmara [Valexport, Petrolina, PE (Brazil); Costa, Maria de Lourdes; Walder, Julio [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Nascimento, Antonio [EMBRAPA Mandioca e Fruticultura, Cruz das Almas, BA (Brazil)

2006-07-01

A key point of the sterile insect technique applied to the medfly, Ceratitis capitata, is that the sterile males produced in the laboratory should have at least a minimal sexual compatibility with wild females. Among several genetic sexing tsl (Temperature Sensitive Lethal) strains of C. capitata mass-reared around the world, the Biofabrica Moscamed Brasil has chosen the most recent mass produced tsl strain, Vienna 8 (V8), which has been evaluated in the San Francisco River Valley, Brazil, since April, 2005. The tests were accomplished in field cages, with different treatments for V8 males, sterile or fertile, exposed to the aroma of ginger root oil (GRO) or not, versus wild males and females. Males of one strain (V8 or wild) were painted white on the thorax the day before the mating tests. All the insects were virgin, and early in the morning (7-8 A.M.) males were released inside the field cages, 10 min. before females. Mating pairs were collected in glass vials, until early afternoon. From this raw data, both the type of male mating and the time in copula were recorded for each pair. Then, the total percentage of mated females, the RSI (Relative Sterility Index), and Fried's competitiveness values (C), were calculated for each field cage. The percentage of females mated was statistically higher to sterile males exposed to GRO than to non exposed to GRO. Time in copula was significantly higher for wild flies than for laboratory flies, except for the case of fertile V8 males exposed to GRO x wild females. The RSI and C values were significantly higher for V8 males (irradiated and fertile) treated with GRO than for V8 males not treated with GRO. The results indicate that there is adequate sexual compatibility between sterile males of the tsl Vienna 8 strain and wild C. capitata females from the San Francisco River Valley, Brazil. Also, the radiation dose of 95 Gy, used to sterilize the males, did not affect their sexual activity. Ginger root oil acted as a

Atoms for pest control

International Nuclear Information System (INIS)

Lindquist, D.A.

1984-01-01

Insects cause losses estimated at between 8% and 20% of total production of crops and livestock throughout the world. With the aim of developing technologies which can reduce such losses, the Insect and Pest Control Section of the Joint FAO/IAEA Division actively sponsors projects and conducts research through the Entomology Section of the Agricultural Biotechnology Laboratory at Seibersdorf. In its work, the Section has placed considerable emphasis on the Sterile Insect Technique (SIT). This technique involves the sterilization and release of large numbers of insects of the target species into the area where control is to be achieved. There, the sterile insects mate with the fertile wild insects, which produce no progeny: the technique is thus a highly specific form of ''birth control''. It is being used against a number of pest species in several countries

Consultants Group Meeting on Rational Supply of Sterile Flies for Medfly SIT in the Mediterranean Basin. Working Material

International Nuclear Information System (INIS)

2000-01-01

The medfly, Ceratitis capitata, is a very damaging insect pest of citrus, stone fruit, pome fruit, figs, etc. in the Mediterranean Basin (MB) - The current insecticide based method for medfly control is causing serious environmental and insecticide residue problems throughout the MB - Producers, exporters, plant protection officials and environmentalists all are seeking ways to reduce insecticide usage against the medfly. - The sterile insect technique (SIT) is a proven method of managing the medfly on an area wide basis. It is the only alternative to repeated insecticide treatments for medfly control. The SIT is environmentally friendly and little or no insecticide is involved in SIT based insect control systems. - The SIT is routinely used against the medfly in Argentina, Chile, Guatemala, Mexico and the USA. - The SIT is not used against the medfly in the MB. There is a medfly control programme being developed in Madeira, Portugal. A demonstration is currently underway in Israel. Valencia, Spain and Algarve, Portugal are planning SIT demonstrations or programmes. Morocco and Corsica (France) are considering SIT efforts. Other MB countries are known to be considering the SIT for use against the medfly. - The major deterrent to using the SIT is lack of confidence that it really is effective and the perceived high cost of the technology. Efficacy has been repeatedly proven in the new world. Cost studies have clearly shown the SIT is not more expensive than insecticide based medfly control systems. Regardless demonstrations projects are required in the MB. - The limiting factor to one or more SIT based medfly control demonstrations in the MB is lack of a source of sufficient numbers of sterile male medflies. The only medfly factory in the MB is in Madeira and is too small to provide sufficient sterile flies. Thus it is imperative that for rapid development of the SIT for medfly control in the MB, a large medfly factory is essential. - The planned (IAEA supported

Gamma radiation for all phases of life cycle of cotton bollworm Helicoverpa armigera aiming at its control

International Nuclear Information System (INIS)

Haddad, Gianni Q.; Arthur, Valter

2017-01-01

Since the 1950s, scientists have used ionizing radiation to sterilize insects, which are released in nature to mate, but without any progeny. Known as the sterile insect technique (SIT), this insect control method traditionally uses ionizing radiations to sterilize insects, being a technique that does not generate residue, and can act in synergy with the other techniques within integrated pest management. For several years, Brazil has been fighting against the increase of pests, introducing new tactics and techniques within IPM programs, to overcome the resistance of chemical products, such as: reducing residues of pesticides. For some important crops of our country, we have a wide spectrum of pests occurring from beginning to end of the harvest, one of them is the cotton crop and among the key pests of this crop, we have some extremely important caterpillars among them Helicoverpa armigera. Due to this the objective this study was establishes doses of gamma radiation to sterilizing of the phases of : eggs, larvae, pupae and adults of H. armigera aiming their control. The experiment was carried out with application of gamma radiation from a Cobalt-60 source. The treatments consisted of doses of gamma radiation varying of according with the insect phase, being this variation of: 0 (control) to 400 Gy. The experiments with pupae and adult phases showed satisfactory results in the sterilization of H. armigera for use in autocide control programs. The sterilize dose to adult and pupae phase were 400 Gy and 100 Gy respectively, being the best doses for the application of the sterile insect technique to this pest in cotton. (author)

Gamma radiation for all phases of life cycle of cotton bollworm Helicoverpa armigera aiming at its control

Energy Technology Data Exchange (ETDEWEB)

Haddad, Gianni Q.; Arthur, Valter, E-mail: ghaddad2001@yahoo.com.br, E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Laboratório de Radiobiologia e Ambiente; Machi, André R., E-mail: rica_machi@hotmail.com [Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), São Paulo, SP (Brazil)

2017-07-01

Since the 1950s, scientists have used ionizing radiation to sterilize insects, which are released in nature to mate, but without any progeny. Known as the sterile insect technique (SIT), this insect control method traditionally uses ionizing radiations to sterilize insects, being a technique that does not generate residue, and can act in synergy with the other techniques within integrated pest management. For several years, Brazil has been fighting against the increase of pests, introducing new tactics and techniques within IPM programs, to overcome the resistance of chemical products, such as: reducing residues of pesticides. For some important crops of our country, we have a wide spectrum of pests occurring from beginning to end of the harvest, one of them is the cotton crop and among the key pests of this crop, we have some extremely important caterpillars among them Helicoverpa armigera. Due to this the objective this study was establishes doses of gamma radiation to sterilizing of the phases of : eggs, larvae, pupae and adults of H. armigera aiming their control. The experiment was carried out with application of gamma radiation from a Cobalt-60 source. The treatments consisted of doses of gamma radiation varying of according with the insect phase, being this variation of: 0 (control) to 400 Gy. The experiments with pupae and adult phases showed satisfactory results in the sterilization of H. armigera for use in autocide control programs. The sterilize dose to adult and pupae phase were 400 Gy and 100 Gy respectively, being the best doses for the application of the sterile insect technique to this pest in cotton. (author)

Product quality control, irradiation and shipping procedures for mass-reared tephritid fruit flies for sterile insect release programmes

International Nuclear Information System (INIS)

1999-05-01

This document represents the recommendations, reached by consensus of an international group of quality control experts, on the standard procedures for product quality control (QC) for mass reared tephritid flies that are to be used in Sterile Insect Technique (SIT) programs. In addition, the manual describes recommended methods of handling and packaging pupae during irradiation and shipment. Most of the procedures were designed specifically for use with Mediterranean fruit flies, Ceratitis capitata (Wied.), but they are applicable, with minor modification in some cases, for other tephritid species such as Caribbean fruit fly Anastrepha suspense, Mexican fruit fly A. ludens, and various Bactrocera species. The manual is evolving and subject to periodic updates. The future additions will include other fruit flies as the need is identified. If followed, procedures described in this manual will help ensure that the quality of mass-produced flies is measured accurately in a standardised fashion, allowing comparisons of quality over time and across rearing facilities and field programmes. Problems in rearing, irradiation and handling procedures, and strain quality can be identified and hopefully corrected before control programmes are affected. Tests and procedures described in this document are only part of a total quality control programme for tephritid fly production. The product QC evaluations included in this manual are, unless otherwise noted, required to be conducted during SIT programmes by the Field programme staff not the production staff. Additional product QC tests have been developed and their use is optional (see ancillary test section). Production and process QC evaluations (e.g., analysis of diet components, monitoring the rearing environment, yield of larvae, development rate, etc.) are not within the scope of this document. Quality specifications are included for minimum and mean acceptability of conventional strains of C. capitata, A. ludens, and A

Handling technique of spore-forming bacteria in radiation sterilization. 1. Preparation of spores

International Nuclear Information System (INIS)

Koshikawa, Tomihiko

1994-01-01

This paper deals with a handling technique of spore-forming bacteria in radiation sterilization. An explanation is given under three sections: (1) life cycle of spore-forming bacteria, medium to form bacterial spores, and colony and purification methods of bacterial spores; (2) methods for measuring the number of bacterial spores and resistance against gamma radiation (D values); and (3) a test method for identifying spore-forming bacteria and a simple identification method. (N.K.)

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds.

Science.gov (United States)

Lindsey, Benson E; Rivero, Luz; Calhoun, Chistopher S; Grotewold, Erich; Brkljacic, Jelena

2017-10-17

Arabidopsis thaliana (Arabidopsis) seedlings often need to be grown on sterile media. This requires prior seed sterilization to prevent the growth of microbial contaminants present on the seed surface. Currently, Arabidopsis seeds are sterilized using two distinct sterilization techniques in conditions that differ slightly between labs and have not been standardized, often resulting in only partially effective sterilization or in excessive seed mortality. Most of these methods are also not easily scalable to a large number of seed lines of diverse genotypes. As technologies for high-throughput analysis of Arabidopsis continue to proliferate, standardized techniques for sterilizing large numbers of seeds of different genotypes are becoming essential for conducting these types of experiments. The response of a number of Arabidopsis lines to two different sterilization techniques was evaluated based on seed germination rate and the level of seed contamination with microbes and other pathogens. The treatments included different concentrations of sterilizing agents and times of exposure, combined to determine optimal conditions for Arabidopsis seed sterilization. Optimized protocols have been developed for two different sterilization methods: bleach (liquid-phase) and chlorine (Cl2) gas (vapor-phase), both resulting in high seed germination rates and minimal microbial contamination. The utility of these protocols was illustrated through the testing of both wild type and mutant seeds with a range of germination potentials. Our results show that seeds can be effectively sterilized using either method without excessive seed mortality, although detrimental effects of sterilization were observed for seeds with lower than optimal germination potential. In addition, an equation was developed to enable researchers to apply the standardized chlorine gas sterilization conditions to airtight containers of different sizes. The protocols described here allow easy, efficient, and

Can Prunus serotina be genetically engineered for reproductive sterility and insect pest resistance?

Science.gov (United States)

Ying Wang; Paula M. Pijut

2014-01-01

Black cherry (Prunus serotina) is a valuable hardwood timber species, and its value highly depends on the wood quality which is often threatened by insect pests. Transgenic black cherry plants that are more resistant to cambial-mining insects may reduce the occurrence of gummosis and have great economic benefits to landowners and the forest products...

Guidance for packing, shipping, holding and release of sterile flies in area-wide fruit fly control programmes

International Nuclear Information System (INIS)

Enkerlin, W.

2007-01-01

This guidance represents the recommendations, reached by consensus of an international group of experts, on the standard procedures for the packing, shipping, holding and release of mass reared and sterilized tephritid flies that are to be used in area-wide programmes that include the Sterile Insect Technique (SIT). The majority of the procedures were initially designed specifically for the Mediterranean fruit fly Ceratitis capitata (Wiedemann) (or Medfly), but they are applicable, with minor modifications, for other tephritid species such as those in the genera Anastrepha, Bactrocera and Dacus. The guidance is designed to be a working document that can be subject to periodic updates due to technological developments and research contributions. Future editions will endeavour to include more specific recommendations for other species of fruit flies as the relevant data become available. The procedures described in this guidance will help ensure that released sterile fruit flies will be of optimal quality and that the resulting field density of these flies will be as closely aligned to the individual programme needs. It is hoped that this guidance will help to quickly identify and correct problems in programme effectiveness, resulting from less than optimal emergence and release conditions

Insect and Pest Control Newsletter, No. 87, July 2016

International Nuclear Information System (INIS)

2016-07-01

A year ago, in NL 85, we reported on the increasing demands from our FAO and IAEA Member States to expand our focus from developing and transferring the sterile insect technique (SIT) for major crop and livestock insect pests to major disease-transmitting mosquitoes. Since the mid-2000s, there have been several IAEA General Conference resolutions requesting the Joint FAO/IAEA Insect Pest Control Subprogramme to develop a complete “SIT package” for major mosquito species to be used as a component of area-wide integrated vector management (IVM) approaches. The first resolutions focussed on the malaria vector Anopheles arabiensis, but since 2010, also the dengue and chikungunya vectors Aedes aegypti and Ae. albopictus were included. In view that the traditional chemical-based vector control strategies were facing serious challenges due to increased resistance of mosquitoes to insecticides and increased public concern of insecticide use in urban areas, there was a clear need for novel methods and complementary approaches to manage mosquito populations in an effective and more environmentally friendly and sustainable way. Furthermore, due to the absence of effective vaccines and drugs against some of these diseases, vector suppression approaches are widely seen as the most effective means to reduce these mosquito-transmitted diseases that pose an enormous economic and social burden, and whose incidence has increased drastically in recent years with the spread to new regions.

Area-wide integration of lepidopteran F1 sterility and augmentative biological control

International Nuclear Information System (INIS)

Carpenter, James E.

2000-01-01

Area-wide pest management (APM) and integrated pest management (IPM) originated from two different efforts to combine two or more control techniques into programmes in which each method could synergise the effectiveness of others and thus create a level of pest control that was greater than that of a single technique (Perkins 1982). Since then, the concept of APM has evolved to include many aspects of IPM and often is now referred to as area-wide IPM. Still, the element of total population management is central to this approach of insect pest management. In support of APM, Knipling (1998) stated that of the insect pests that were of major concern to agriculture before the newer classes of insecticides were available, most are still pests today, the major exceptions being the screw-worm fly and the boll weevil in the southeastern US cotton growing region. Knipling also noted that both of these pest species were subjected to area-wide suppression programmes. In response to the USDA IPM Initiative (USDA 1993, 1994) which seeks to achieve the national goal of having 75% of the crop acres under IPM by the year 2000, the Agricultural Research Service developed an Area-wide IPM Programme. This programme combines environmentally-sound pest control techniques with the advantages of APM and develops partnerships with other federal, state, local and private sector entities. Technologies such as the integration of lepidopteran F 1 sterility and augmentative biological control may be considered for future programmes

Dynamic sterilization of titanium implants with ultraviolet light

International Nuclear Information System (INIS)

Singh, S.; Schaaf, N.G.

1989-01-01

All implantable devices must be sterile. However, autoclaves produce poor surface properties that jeopardize the integration process. The application of a modified ultraviolet light source has proven to enhance bioreactivity by controlling surface properties, but it lacks validation of its sterilization capabilities. Forty-eight titanium implants were contaminated with spores of the biological indicator Bacillus stearothermophilus and subjected to dynamic sterilization by ultraviolet light. Forty-seven of the implants were successfully sterilized, as indicated by not producing turbidity in a suitable growth medium. This sterilization technique only requires a 20-second exposure to achieve sterility

Sterilization by oxygen plasma

Energy Technology Data Exchange (ETDEWEB)

Moreira, Adir Jose; Mansano, Ronaldo Domingues; Andreoli Pinto, Terezinha de Jesus; Ruas, Ronaldo; Silva Zambon, Luis da; Silva, Monica Valero da; Verdonck, Patrick Bernard

2004-07-31

The use of polymeric medical devices has stimulated the development of new sterilization methods. The traditional techniques rely on ethylene oxide, but there are many questions concerning the carcinogenic properties of the ethylene oxide residues adsorbed on the materials after processing. Another common technique is the gamma irradiation process, but it is costly, its safe operation requires an isolated site and it also affects the bulk properties of the polymers. The use of a gas plasma is an elegant alternative sterilization technique. The plasma promotes an efficient inactivation of the micro-organisms, minimises the damage to the materials and presents very little danger for personnel and the environment. Pure oxygen reactive ion etching type of plasmas were applied to inactivate a biologic indicator, the Bacillus stearothermophilus, to confirm the efficiency of this process. The sterilization processes took a short time, in a few minutes the mortality was complete. In situ analysis of the micro-organisms' inactivating time was possible using emission spectrophotometry. The increase in the intensity of the 777.5 nm oxygen line shows the end of the oxidation of the biologic materials. The results were also observed and corroborated by scanning electron microscopy.

Breakfast of champions or kiss of death? Survival and sexual performance of protein-fed, sterile Mediterranean fruit flies (Diptera: Tephritidae)

International Nuclear Information System (INIS)

Yuval, B.; Maor, M.; Levy, K.; Kaspi, R.; Taylor, P.; Shelly, T.

2007-01-01

The sterile insect technique (SIT) is increasingly being used around the world to control Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), the Mediterranean fruit fly as part of an area-wide integrated approach. One option that may improve the effectiveness of the SIT, by increasing the sexual competitiveness of released sterile males, consists of feeding males protein during the post-teneral stage, a diet that increases sexual performance of wild males. We examine the effects of diet on the successive hurdles males must overcome in order to inseminate females, i.e., joining leks, copulating females, having their sperm stored and inhibition of female remating. In addition, we address the effects of diet on post-release foraging success, longevity, and the ability to withstand starvation. While protein feeding universally increases the sexual success of wild males, its effect on sterile males varies with strain, experimental settings, and environmental conditions. In some cases, treatments that resulted in the best sexual performance were significantly associated with increased vulnerability to starvation. However, no particular diet affected the ability of sterile males to find nutrients in the field when these where available. We suggest it may be better to release relatively short-lived flies that are highly competitive, rather than long-lived, sexually ineffective ones. (author) [es

Dispersion of fruit flies (Diptera: Tephritidae) at high and low densities and consequences of mismatching dispersions of wild and sterile flies

International Nuclear Information System (INIS)

Meats, A.

2007-01-01

Both wild and released (sterile) Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) and wild Bactrocera papayae (Drew and Hancock) in Australia had patchy distributions and comparisons with predictions of the negative binomial model indicated that the degree of clumping was sometimes very high, particularly at low densities during eradication. An increase of mean recapture rate of sterile B. tryoni on either of 2 trap arrays was not accompanied by a reduction in its coefficient of variation and when recapture rates were high, the percentage of traps catching zero decreased only slightly with increase in recapture rate, indicating that it is not practicable to decrease the heterogeneity of dispersion of sterile flies by increasing the number released. There was often a mismatch between the dispersion patterns of the wild and sterile flies, and the implications of this for the efficiency of the sterile insect technique (SIT) were investigated with a simulation study with the observed degrees of mismatch obtained from the monitoring data and assuming the overall ratio of sterile to wild flies to be 100:1. The simulation indicated that mismatches could result in the imposed rate of increase of wild flies being up to 3.5 times higher than that intended (i.e., 0.35 instead of 0.1). The effect of a mismatch always reduces the efficiency of SIT. The reason for this asymmetry is discussed and a comparison made with host-parasitoid and other systems. A release strategy to counter this effect is suggested. (author) [es

First COSTECH scientific and technological conference: Science and technology for growth and poverty reduction in Tanzania

International Nuclear Information System (INIS)

Malele, I.I.

2006-01-01

Sterile Insect Technique (SIT) is a form of insect birth control that is based on the introduction of sterility in an insect population by radio sterilization and is best when carried out on an area-wide basis to control both insect pest and vectors which threatens agricultural expansion and diversification. In Tanzania as is the case in many other African countries, insect pest and vectors have adverse effect on farm productivity, undermine farm produce competitiveness in the market and unnecessarily limits the country and her people's prosperity. Apart from having adverse effect on the agricultural sector, Insect pest and vectors cause diseases and annoyance on human beings. (author)

Laboratory longevity and competitiveness of Dacus ciliatus Loew (Diptera: Tephritidae) following sub-sterilizing gamma irradiation.

Science.gov (United States)

Nemny-Lavy, E; Nestel, D; Rempoulakis, P

2016-06-01

The effect of a sub-sterilizing gamma radiation dose on Dacus ciliatus adults was investigated to assess the suitability of the sterile insect technique (SIT) as an alternative method to control this pest. Late pupae (48 h prior to adult emergence) from a laboratory strain were irradiated with 120 Gy of gamma rays emitted by a 60Co source. Following adult emergence, the mortality of irradiated and non-irradiated cohorts was recorded. Over a period of 50 days after emergence, no significant negative effects of irradiation upon the longevity of male or female laboratory flies were observed. A laboratory competitiveness study (Fried test), using irradiated laboratory and wild males at a ratio of 3:1 was conducted to assess the ability of irradiated males to reduce the egg hatch rates of a wild population. The overall competitiveness was found to be ca. 0.32, suggesting a reduced, but satisfactory, quality of irradiated laboratory as compared with wild males. Based on the above findings, we calculated and proposed effective male release ratios for field application of SIT against D. ciliatus.

Supercritical Carbon Dioxide–Based Sterilization of Decellularized Heart Valves

Directory of Open Access Journals (Sweden)

Ryan S. Hennessy, MD

2017-02-01

Full Text Available Summary: Sterilization of grafts is essential. Supercritical carbon dioxide, electrolyzed water, gamma radiation, ethanol-peracetic acid, and hydrogen peroxide techniques were compared for impact on sterility and mechanical integrity of porcine decellularized aortic valves. Ethanol-peracetic acid– and supercritical carbon dioxide–treated valves were found to be sterile using histology, microbe culture, and electron microscopy assays. The cusp tensile properties of supercritical carbon dioxide–treated valves were higher compared with valves treated with other techniques. Superior sterility and integrity was found in the decellularized valves treated with supercritical carbon dioxide sterilization. This sterilization technique may hold promise for other decellularized soft tissues. Key Words: decellularized, decontamination, heart valve, tensile properties, tissue engineering

A Novel Technique for Sterilization Using a Power Self-Regulated Single-Mode Microwave Cavity.

Science.gov (United States)

Reverte-Ors, Juan D; Pedreño-Molina, Juan L; Fernández, Pablo S; Lozano-Guerrero, Antonio J; Periago, Paula M; Díaz-Morcillo, Alejandro

2017-06-07

In this paper, a novel technique to achieve precise temperatures in food sterilization has been proposed. An accurate temperature profile is needed in order to reach a commitment between the total removal of pathogens inside the product and the preservation of nutritional and organoleptic characteristics. The minimal variation of the target temperature in the sample by means of a monitoring and control software platform, allowing temperature stabilization over 100 °C, is the main goal of this work. A cylindrical microwave oven, under pressure conditions and continuous control of the microwave supply power as function of the final temperature inside the sample, has been designed and developed with conditions of single-mode resonance. The uniform heating in the product is achieved by means of sample movement and the self-regulated power control using the measured temperature. Finally, for testing the sterilization of food with this technology, specific biological validation based on Bacillus cereus as a biosensor of heat inactivation has been incorporated as a distribution along the sample in the experimental process to measure the colony-forming units (CFUs) for different food samples (laboratory medium, soup, or fish-based animal by-products). The obtained results allow the validation of this new technology for food sterilization with precise control of the microwave system to ensure the uniform elimination of pathogens using high temperatures.

A Novel Technique for Sterilization Using a Power Self-Regulated Single-Mode Microwave Cavity

Directory of Open Access Journals (Sweden)

Juan D. Reverte-Ors

2017-06-01

Full Text Available In this paper, a novel technique to achieve precise temperatures in food sterilization has been proposed. An accurate temperature profile is needed in order to reach a commitment between the total removal of pathogens inside the product and the preservation of nutritional and organoleptic characteristics. The minimal variation of the target temperature in the sample by means of a monitoring and control software platform, allowing temperature stabilization over 100 °C, is the main goal of this work. A cylindrical microwave oven, under pressure conditions and continuous control of the microwave supply power as function of the final temperature inside the sample, has been designed and developed with conditions of single-mode resonance. The uniform heating in the product is achieved by means of sample movement and the self-regulated power control using the measured temperature. Finally, for testing the sterilization of food with this technology, specific biological validation based on Bacillus cereus as a biosensor of heat inactivation has been incorporated as a distribution along the sample in the experimental process to measure the colony-forming units (CFUs for different food samples (laboratory medium, soup, or fish-based animal by-products. The obtained results allow the validation of this new technology for food sterilization with precise control of the microwave system to ensure the uniform elimination of pathogens using high temperatures.

Light microscopy with differential staining techniques for the characterisation and discrimination of insects versus marine arthropods processed animal proteins.

Science.gov (United States)

Ottoboni, Matteo; Tretola, Marco; Cheli, Federica; Marchis, Daniela; Veys, Pascal; Baeten, Vincent; Pinotti, Luciano

2017-08-01

The aim of this study was to evaluate the use of light microscopy with differential staining techniques for the discrimination of insect material from marine arthropods - classified as fishmeal. Specifically, three samples of single-species insect material, Hermetia illucens (HI), Bombyx mori (BM) and Tenebrio molitor (TM), and two samples of marine arthropods, shrimp material and krill, were analysed and compared after staining by two reagents to enhance fragment identification. Alizarin Red (AR) and Chlorazol Black (CB), which react respectively with calcium salts and chitin, were tested for their potential efficacy in distinguishing between insect and marine materials. Results indicated that AR failed to stain HI, BM and TM materials. By contrast, the three insect species materials tested were stained by CB. When shrimp fragments and krill were considered, AR and CB stained marine materials reddish-pink and light blue to black, respectively. By combining these results, it can be suggested that CB staining may efficiently be used to mark insect materials; AR does stain shrimp fragments but does not stain the tested insect material, indicating a possible approach for discriminating between insects and marine arthropods. However, since the present study was performed on pure materials and a small set of samples, possible implementation of this technique still needs to be confirmed in complex matrices such as compound feed.

Insect pest control newsletter. No. 61

International Nuclear Information System (INIS)

2003-07-01

this sexing system, which could be a model for other moth pest insects. Germ-line transformation is quite efficient in the codling moth and the appropriate Notch allele has already been shown by Lisa Neven at ARS/USDA in Wapato, Washington State to express correctly in transformed codling moth lines. If such a sexing system could be developed in codling moth then it would have several advantages, a) only the females would be transgenic, the sterile males to be released as part of SIT operations would carry a completely normal genome, b) as the Notch gene is dominant, any recombinant individuals would also be killed by the temperature treatment and not be accidentally released, c) the fluorescent marker would be very tightly linked to Notch, resulting in strain stability, d) stockpiles of diapausing mass reared males would require only half the storage space, and e) the system should probably be easily transferable to other economically important lepidopteran pests. I would also like to report developments related to medfly control in Europe. Currently, medfly control requires intensive insecticide applications during the whole fruit maturation period. This is also the case of southern Spain, where the largest citrus production areas in Europe are located. Nevertheless, as a result of the detection of medfly-infested cargo at US ports of entry over a year ago, the US phytosanitary authorities banned temporarily the large citrus exports from Valencia, Spain. As the US has expensive programmes in operation to remain medly-free, including various preventive SIT programmes, it cannot tolerate the import of infested fruit. In view of these developments, and in order to reduce insecticide use to protect the environment, the authorities of the Autonomous Province of Valencia decided in 2002 to gradually shift their medfly control strategy from conventional large scale aerial insecticide spraying to an integrated pest control approach including the SIT. In March 2002, FAO

Sewage sterilization through gamma radiation

International Nuclear Information System (INIS)

Vieira, D.V.; Teixeira, W.M.S.; Silva, F.C.A. da

2017-01-01

Lack of sewage treatment and adequate sanitation conditions can contribute to the proliferation of numerous parasitic and infectious diseases in addition to water degradation. Approximately fifty types of infections can be transmitted from a sick to a healthy person through different pathways involving human excreta. Untreated sewage can contaminate water, food, hands, soil, etc. Epidemics of certain diseases such as typhoid, cholera, dysenteries, etc., and countless cases of worms are responsible for high mortality rates in third world countries. In the work the different techniques of sewage treatment by disinfestation and sterilization were analyzed, highlighting the use and the advantages of the gamma radiation as well as the aspects of the radiological protection involved. The technique of sewage sterilization using gamma radiation is a method of controlling bacteria and microorganisms. It is estimated that more than 200 large irradiators are in operation worldwide, of which 5 are in Brazil, for general sterilization use. These facilities use a large amount of radioactive material, in the order of millions of Becquerel, for sterilization with high doses of radiation, which can generate lethal doses in a few minutes. These industrial facilities use Cobalt-60, being classified by the International Atomic Energy Agency - IAEA as Category 1 of high risk, and must possess a high level of radiological protection to carry out the sterilization, standing out the defense in depth. Specific legislation on radiological protection should be drafted for safe work and avoid future radiation accidents

Mating competitiveness of male Anopheles arabiensis mosquitoes irradiated with a partially or fully sterilizing dose in small and large laboratory cages.

Science.gov (United States)

Helinski, M E H; Knols, B G J

2008-07-01

Male mating competitiveness is a crucial parameter in many genetic control programs including the sterile insect technique (SIT). We evaluated competitiveness of male Anopheles arabiensis Patton as a function of three experimental variables: (1) small or large cages for mating, (2) the effects of either a partially sterilizing (70 Gy) or fully sterilizing (120 Gy) dose, and (3) pupal or adult irradiation. Irradiated males competed for females with an equal number of unirradiated males. Competitiveness was determined by measuring hatch rates of individually laid egg batches. In small cages, pupal irradiation with the high dose resulted in the lowest competitiveness, whereas adult irradiation with the low dose gave the highest, with the latter males being equal in competitiveness to unirradiated males. In the large cage, reduced competitiveness of males irradiated in the pupal stage was more pronounced compared with the small cage; the males irradiated as adults at both doses performed similarly to unirradiated males. Unexpectedly, males irradiated with the high dose performed better in a large cage than in a small one. A high proportion of intermediate hatch rates was observed for eggs collected in the large cage experiments with males irradiated at the pupal stage. It is concluded that irradiation of adult An. arabiensis with the partially sterilizing dose results in the highest competitiveness for both cage designs. Cage size affected competitiveness for some treatments; therefore, competitiveness determined in laboratory experiments must be confirmed by releases into simulated field conditions. The protocols described are readily transferable to evaluate male competitiveness for other genetic control techniques.

Insect compassion, evidence of altruism, reciprocity, and midwifery behavior in aphids

Science.gov (United States)

Altruism is characterized by an act provided by a donor to a recipient that is considered detrimental to the donor yet benefits the recipient. Evidence of altruism is abundant in nature. In insects, altruism is manifest by ant and bee colonies where sterile workers provide labor, care of young, co...

Degradation of chitosan-based materials after different sterilization treatments

International Nuclear Information System (INIS)

San Juan, A; Montembault, A; Royaud, I; David, L; Gillet, D; Say, J P; Rouif, S; Bouet, T

2012-01-01

Biopolymers have received in recent years an increasing interest for their potential applications in the field of biomedical engineering. Among the natural polymers that have been experimented, chitosan is probably the most promising in view of its exceptional biological properties. Several techniques may be employed to sterilize chitosan-based materials. The aim of our study was to compare the effect of common sterilization treatments on the degradation of chitosan-based materials in various physical states: solutions, hydrogels and solid flakes. Four sterilization methods were compared: gamma irradiation, beta irradiation, exposure to ethylene oxide and saturated water steam sterilization (autoclaving). Exposure to gamma or beta irradiation was shown to induce an important degradation of chitosan, regardless of its physical state. The chemical structure of chitosan flakes was preserved after ethylene oxide sterilization, but this technique has a limited use for materials in the dry state. Saturated water steam sterilization of chitosan solutions led to an important depolymerization. Nevertheless, steam sterilization of chitosan flakes bagged or dispersed in water was found to preserve better the molecular weight of the polymer. Hence, the sterilization of chitosan flakes dispersed in water would represent an alternative step for the preparation of sterilized chitosan solutions. Alternatively, autoclaving chitosan physical hydrogels did not significantly modify the macromolecular structure of the polymer. Thus, this method is one of the most convenient procedures for the sterilization of physical chitosan hydrogels after their preparation.

The influence of sterilization on nitrogen-included ultrananocrystalline diamond for biomedical applications

International Nuclear Information System (INIS)

Tong, Wei; Tran, Phong A.; Turnley, Ann M.; Aramesh, Morteza; Prawer, Steven; Brandt, Milan; Fox, Kate

2016-01-01

Diamond has shown great potential in different biomedical applications, but the effects of sterilization on its properties have not been investigated. Here, we studied the influence of five sterilization techniques (solvent cleaning, oxygen plasma, UV irradiation, autoclave and hydrogen peroxide) on nitrogen-included ultrananocrystalline diamond. The chemical modification of the diamond surface was evaluated using X-ray photoelectron spectroscopy and water contact angle measurements. Different degrees of surface oxidation and selective sp 2 bonded carbon etching were found following all sterilization techniques, resulting in an increase of hydrophilicity. Higher viabilities of in vitro mouse 3T3 fibroblasts and rat cortical neuron cells were observed on oxygen plasma, autoclave and hydrogen peroxide sterilized diamond, which correlated with their higher hydrophilicity. By examination of apatite formation in simulated body fluid, in vivo bioactivity was predicted to be best on those surfaces which have been oxygen plasma treated and lowest on those which have been exposed to UV irradiation. The charge injection properties were also altered by the sterilization process and there appears to be a correlation between these changes and the degree of oxygen termination of the surface. We find that the modification brought by autoclave, oxygen plasma and hydrogen peroxide were most consistent with the use of N-UNCD in biological applications as compared to samples sterilized by solvent cleaning or UV exposure or indeed non-sterilized. A two-step process of sterilization by hydrogen peroxide following oxygen plasma treatment was then suggested. However, the final choice of sterilization technique will depend on the intended end application. - Highlights: • We test for the first time the effect of 5 sterilization techniques on nitrogen included ultrananocrystalline diamond. • Different degrees of surface oxidation and selective sp 2 bonded carbon etching were found to

The influence of sterilization on nitrogen-included ultrananocrystalline diamond for biomedical applications

Energy Technology Data Exchange (ETDEWEB)

Tong, Wei [School of Physics, University of Melbourne, Parkville, Victoria (Australia); Tran, Phong A. [Department of Chemical and Biomolecular Engineering, University of Melbourne, Parkville, Victoria (Australia); Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland (Australia); Turnley, Ann M. [Department of Anatomy and Neuroscience, University of Melbourne, Parkville, Victoria (Australia); Aramesh, Morteza [School of Physics, University of Melbourne, Parkville, Victoria (Australia); School of Chemistry, Physics, and Mechanical Engineering, Queensland University of Technology, Brisbane, Queensland (Australia); Prawer, Steven [School of Physics, University of Melbourne, Parkville, Victoria (Australia); Brandt, Milan [Centre for Additive Manufacturing, School of Aerospace, Mechanical and Manufacturing Engineering, RMIT University, Melbourne, Victoria (Australia); Fox, Kate, E-mail: kate.fox@rmit.edu.au [Centre for Additive Manufacturing, School of Aerospace, Mechanical and Manufacturing Engineering, RMIT University, Melbourne, Victoria (Australia)

2016-04-01

Diamond has shown great potential in different biomedical applications, but the effects of sterilization on its properties have not been investigated. Here, we studied the influence of five sterilization techniques (solvent cleaning, oxygen plasma, UV irradiation, autoclave and hydrogen peroxide) on nitrogen-included ultrananocrystalline diamond. The chemical modification of the diamond surface was evaluated using X-ray photoelectron spectroscopy and water contact angle measurements. Different degrees of surface oxidation and selective sp{sup 2} bonded carbon etching were found following all sterilization techniques, resulting in an increase of hydrophilicity. Higher viabilities of in vitro mouse 3T3 fibroblasts and rat cortical neuron cells were observed on oxygen plasma, autoclave and hydrogen peroxide sterilized diamond, which correlated with their higher hydrophilicity. By examination of apatite formation in simulated body fluid, in vivo bioactivity was predicted to be best on those surfaces which have been oxygen plasma treated and lowest on those which have been exposed to UV irradiation. The charge injection properties were also altered by the sterilization process and there appears to be a correlation between these changes and the degree of oxygen termination of the surface. We find that the modification brought by autoclave, oxygen plasma and hydrogen peroxide were most consistent with the use of N-UNCD in biological applications as compared to samples sterilized by solvent cleaning or UV exposure or indeed non-sterilized. A two-step process of sterilization by hydrogen peroxide following oxygen plasma treatment was then suggested. However, the final choice of sterilization technique will depend on the intended end application. - Highlights: • We test for the first time the effect of 5 sterilization techniques on nitrogen included ultrananocrystalline diamond. • Different degrees of surface oxidation and selective sp{sup 2} bonded carbon etching were

A study on the spreading ability of radiation-sterilized male moth of bombyx mandarin in field

International Nuclear Information System (INIS)

Yang Rongxin; Xia Darong; Gu Weiping; Zhang Yanjun

1998-01-01

The mulberry wild silkworm (MWS), belong to the Bombycidae of Lepidoptera, is serious pest of sericulture. The female moth of MWS is sterile and the male moth is sub-sterile when they were treated with 250 Gy 60 Co γ-ray (dose rate: 1.05 Gy/min), and their filial generations was sterile. The spreading ability of male moth of MWS in field and retrieving the marked MWS male moth with the trapping method was studied. The trapping solution was composed of sugar, vinegar, wine and alive female moth. The retrieving rate of MWS male moth amounted to 12.6%∼13.5% of released moth in field. The spreading range in 24 hours for sterile MWS male moth reached to 700 m, and 90.8% of MWS male moth was in an area of 5 m radius from the releasing centre. It is concluded that thirty releasing centres per hectare are needed to make the irradiated sterilized insects spread for controlling the MWS in field

Genetics and ecology of colonization and mass rearing of Hawaiian fruit flies (Diptera: Tephritidae) for use in sterile insect control programs

International Nuclear Information System (INIS)

Saul, S.H.; McCombs, S.D.

1995-01-01

It is critical to maintain the genetic, physiological and behavioral competence of colonized populations of insect species, such as fruit flies, which are reared for release in sterile insect and other genetic control programs. Selective pressures associated with the mass rearing process affect this competence, but the underlying mechanisms of genetic change arc largely unknown. However, competence is often an operational goal achieved by manipulating environmental factors without possessing precise genetic knowledge of alleles and their marginal effects on the desired traits. One goal of this paper is to show that the precise genetic and statistical analysis of components that determine competence in a broad sense or fitness in the narrower ecological sense, is extremely difficult. We can gel contradictory results from the different methods for estimating genetic variation in tephritid populations. We observe low levels of allozyme variation, but high levels of recessive mutants in inbred populations. We propose that genetic variability may be maintained in colonized and mass reared laboratory populations by balanced lethal systems and that the introduction of fresh genetic material may reduce, not increase, fitness. We require rigorous and precise models of directional selection in the laboratory and selective forces in the natural environment to aid our understanding of dynamic changes in courtship and mating behavior under artificial conditions. We have chosen to examine the lek model as an example of an idea whose usefulness has yet to be determined by test ing and validation. The inclusion of lek forming ability in genetic models will be depen dent on rigorously establishing the validity of the lek model for each tephritid species

Insect and pest control newsletter. No. 58

International Nuclear Information System (INIS)

2002-01-01

This issue of the Newsletter announces the development of a draft international standard to facilitate the transboundary shipment of sterile insects stands out. This was developed in response to requests from Member States and the private sector for regulation of the shipping of sterile insects. The draft standard will be considered, reviewed and hopefully endorsed over the next years by the Interim Commission on Phytosanitary Measures (ICPM), the governing body of the International Plant protection Convention (IPPC). Also of significance are the Fruit Fly Trapping Guidelines that have been developed to support the harmonization of monitoring procedures for these pest insects in view of the increasing fruit fly related transboundary interactions resulting from the rapidly growing trade in agricultural commodities, as well as travel, transport and tourism. An upcoming event also in the normative area is an FAO/IAEA Expert Meeting on 'Risk Assessment of Transgenic Arthropods' to be held at FAO, Rome from 8-12 April, 2002. The objective of the meeting are to a) assess current status of transgenesis in pest arthropods; b) to assess biosafety concerns for transgenic arthropod release; c) to provide guidance for future risk assessment protocols for case by case analysis; and d) to assess the possibility of establishing a working group under IPPC for setting guidelines for development and use of transgenic insect technology. An important event at the end of 2001 was the Resolution on the Pan African Tsetse and Trypanosomosis Eradication Campaign (PATTEC) adopted by the FAO Conference held in Rome, 2-13 November 2001 (for the full text of the resolution see page 39).. The resolution acknowledges the severity of the trypanosomosis problem in sub-Saharan Africa, and the potential benefits of tsetse elimination, and calls upon affected member nations to include tsetse eradication in their Poverty Reduction Strategy Papers and for the FAO to support them in their efforts to

Postpartum Sterilization

Science.gov (United States)

f AQ FREQUENTLY ASKED QUESTIONS FAQ052 CONTRACEPTION Postpartum Sterilization • What is sterilization? • What is postpartum sterilization? • How is postpartum sterilization performed? • What kind of anesthesia is used for postpartum sterilization? • How ...

Using GPS instruments and GIS techniques in data management for insect pest control programs

International Nuclear Information System (INIS)

2006-01-01

This interactive tutorial CD entitled 'Using GPS Instruments and GIS Techniques in Data Management for Insect Pest Control Programs' was developed by Micha silver of the Arava Development Co., Sapir, Israel, and includes step-by-step hands on lessons on the use of GPS/GIS in support of area-wide pest control operations

A farewell to tsetse

International Nuclear Information System (INIS)

1997-01-01

The tsetse fly, a blood sucking insect which transmits a lethal disease, Trypanosomiasis, to animals and people, is found in vast areas of Africa. A promising method to get rid of this pest is the sterile insect technique where millions of sterile but competitive male flies break the reproductive cycle. This technique was successfully used on the island of Zanzibar for the eradication of tsetse fly in 1996

Hysteroscopic Sterilization

Science.gov (United States)

... sterilization? Sterilization is a permanent form of birth control. What is tubal sterilization? Sterilization procedures for women are ... is quicker than from other types of sterilization. What are the risks of ... on for birth control. • There is a risk of injury to the ...

Radioactive labelling of insects

International Nuclear Information System (INIS)

Thygesen, Th.

Experiments are described with the internal contamination of insects with phosphorus 32 introduced previously in plants of the brassica type using three different techniques. The intake of radioactivity from the plants to the insects is shown. (L.O.)

Insect and Pest Control Newsletter, No. 86, January 2016

International Nuclear Information System (INIS)

2016-01-01

In 2015 we concluded the six-year Coordinated Research Project (CRP) on “Resolution of Cryptic Species Complexes of Tephritid Pests to Overcome Constraints to SIT Application and International Trade”. The objective of the CRP was to undertake targeted research into the systematics and diagnostics of taxonomically challenging fruit fly groups of economic importance. Close to 50 researchers from over 20 countries participated in the CRP, conducting coordinated, multidisciplinary research to address, with an integrative taxonomic framework, cryptic species complexes of major tephritid pests. One of the scientific outputs of the CRP was the accurate alignment of some biological species with taxonomic names. The resolution of some of these controversial issues has important applied implications for FAO and IAEA Member States, both in overcoming technical constraints to the application of the Sterile Insect Technique (SIT) against pest fruit flies and in facilitating international agricultural trade

Sterilization of Helicoverpa armigera (hubner) moth (lepidoptera; Noctudae) by using Gamma rays radiation

International Nuclear Information System (INIS)

Subiyakto; Wari D Astati; Sunarto Dwi A

1998-01-01

This experiment was conducted at the Radiation Facility, National Atomic Energy, Jakarta and Entomology Laboratory of the Research Institute for Tobacco and Crops Malang in 1991. Sterilization of moth by radiating insect on pupae stage. The dosage were 0 (unradiated), 1,3,5,7,9,11,13,and 15 krad. The treatments were arranged in complete randomized design with three replications. Each treatments were made variation of copulation (1) two females radiated with a make unradiated (2) two females unradiated with a male radiated, and (3) two females radiated with a male radiated. The results showed that sub sterile dosage (LD 50 ) for male was 0.94 krad and 7.02 krad for female. The sterile dosage (LD 95 ) for male was 10.85 krad and 14.35 krad for female. Research of the competition for copulation between the male radiated and unradiated is needed. (author)

Effectiveness and feasibility of hysteroscopic sterilization techniques: a systematic review and meta-analysis

NARCIS (Netherlands)

la Chapelle, C.F.; Veersema, S.; Brölmann, H.A.M.; Jansen, F.W.

2015-01-01

Objective To assess whether hysteroscopic sterilization is feasible and effective in preventing pregnancy. Secondarily, to identify risk factors for failure of hysteroscopic sterilization. Design A systematic review and meta-analysis. Setting Not applicable. Patient(s) Women undergoing hysteroscopic

Heat sterilization time of ponderosa pine and Douglas-fir boards and square timbers

Science.gov (United States)

William T. Simpson; Xiping Wang; Steve Verrill

2003-01-01

To prevent the unintentional transfer of insects and pathogens during world trade, wood products are often heat sterilized. The general requirement is that the center of the wood configuration be held at 133°F (56°C) for 30 min. However, many factors can affect the time required to reach this temperature. This study explored several of these factors, including...

Spore-Forming Bacteria that Resist Sterilization

Science.gov (United States)

LaDuc, Myron; Venkateswaran, Kasthuri

2003-01-01

A report presents a phenotypic and genotypic characterization of a bacterial species that has been found to be of the genus Bacillus and has been tentatively named B. odysseensis because it was isolated from surfaces of the Mars Odyssey spacecraft as part of continuing research on techniques for sterilizing spacecraft to prevent contamination of remote planets by terrestrial species. B. odysseensis is a Gram-positive, facultatively anaerobic, rod-shaped bacterium that forms round spores. The exosporium has been conjectured to play a role in the elevated resistance to sterilization. Research on the exosporium is proposed as a path toward improved means of sterilization, medical treatment, and prevention of biofouling.

The many selves of social insects.

Science.gov (United States)

Queller, David C; Strassmann, Joan E

2002-04-12

Social insects show multiple levels of self identity. Most individuals are sterile workers who selflessly labor for their colony, which is often viewed as a superorganism. The superorganism protects itself with colony recognition systems based on learned odors, typically cuticular hydrocarbons. Transfer of these odors within the colony obscures separate clan identities. Residual individual interests do appear to cause conflicts within colonies over sex ratio, male production, caste, and reproductive dominance. However, genomic imprinting theory predicts that the individual's maternal and paternal genes will evolve separate infraorganismal identities, perhaps leaving virtually no coherent individual identity.

Transient population dynamics of mosquitoes during sterile male releases: modelling mating behaviour and perturbations of life history parameters.

Directory of Open Access Journals (Sweden)

Christopher M Stone

Full Text Available The release of genetically-modified or sterile male mosquitoes offers a promising form of mosquito-transmitted pathogen control, but the insights derived from our understanding of male mosquito behaviour have not fully been incorporated into the design of such genetic control or sterile-male release methods. The importance of aspects of male life history and mating behaviour for sterile-male release programmes were investigated by projecting a stage-structured matrix model over time. An elasticity analysis of transient dynamics during sterile-male releases was performed to provide insight on which vector control methods are likely to be most synergistic. The results suggest that high mating competitiveness and mortality costs of released males are required before the sterile-release method becomes ineffective. Additionally, if released males suffer a mortality cost, older males should be released due to their increased mating capacity. If released males are of a homogenous size and size-assortative mating occurs in nature, this can lead to an increase in the abundance of large females and reduce the efficacy of the population-suppression effort. At a high level of size-assortative mating, the disease transmission potential of the vector population increases due to male releases, arguing for the release of a heterogeneously-sized male population. The female population was most sensitive to perturbations of density-dependent components of larval mortality and female survivorship and fecundity. These findings suggest source reduction might be a particularly effective complement to mosquito control based on the sterile insect technique (SIT. In order for SIT to realize its potential as a key component of an integrated vector-management strategy to control mosquito-transmitted pathogens, programme design of sterile-male release programmes must account for the ecology, behaviour and life history of mosquitoes. The model used here takes a step in this

Insect pest control newsletter. No. 63

International Nuclear Information System (INIS)

2004-07-01

The Second International Conference on Areawide Insect Pest Control sponsored by FAO and IAEA will be held from 9 to 13 May, 2005 in Vienna, Austria. This conference will provide a forum for the presentation of scientific papers dealing with areawide insect management programmes, including those applying the Sterile Insect Technique (SIT) and will include significant time for plenary discussion. The framework of the conference is being developed and the announcement with details of the Conference can be found under http://www.pub.iaea.org/MTCD/Meetings/Meetings2005.asp. It is planned to hold several Research Coordination Meetings in conjunction with this meeting. The Interregional Training Course on The Use of the Sterile Insect and Related Techniques for the Integrated Areawide Management of Insect Pests, was held from 4 May to 1 June 2004 in Gainesville, Florida, USA. This is a unique course that provides participants with a complete overview of all aspects related to areawide and SIT operational programmes. Both USA and external lecturers participated with an adequate balance between theory and practical laboratory and field exercises. Third, the SIT programme in Madeira is in negotiations with a private company regarding some type of partnership to ensure sustainability of the programme when EC funding comes to an end. These developments have been followed very closely by the sub-programme and we have been involved in providing advice, developing collaborative links and interacting at the R and D and technology transfer levels. There will be ample scope for further collaboration when these initiatives become fully realized. The fifth meeting of the Working Group on Fruit Flies of the Western Hemisphere (WGFFWH) took place in Fort Lauderdale, Florida, from 16 to 21 May 2004 and more than 200 participants attended. The meeting has a very unique format where scientists, action programme managers and the industry interact, greatly encouraging discussions and

Application of radioisotopes in plant protection

International Nuclear Information System (INIS)

Pothiraj, G.; Pavani, Lalitha; Allimuthu, Elangovan; Yadav, Ranjeet Singh

2017-01-01

There are concerns that continuous uses of pesticides have negative impacts on the environment and it also results in development of resistance against pesticides in many insect species. Radioactive exposure improves quality and productivity of agricultural products along with insect, pest and disease management. They are helpful in study of optimum utilization of insecticides and pesticides in cultivated crops without harmful effects to plants and other living organisms. The control of plant virus diseases is one of the greatest problems of our day. Aphids feeding on plants containing 32 P become radioactive and retain the label for some weeks which helps in monitoring their movement. Sterile Insect Technique (SIT) proposed by Knipling in 1955, relies on application of ionizing radiation as a means to effectively sterilize male insects without affecting their ability to function in the field and successfully mate with wild female insects. This technique involves release of large numbers of sterile male insects of the target species in the field crop. With relevance to plant pathogens, radioisotopes are used to measure the spread of the plant diseases. (author)

Application of radiation sterilization to bone allografts

International Nuclear Information System (INIS)

Li Youchen; Li Baoxing; Sun Shiquan

2003-01-01

With prominent features of high penetration, no temperature increases, no harm residues and easy dose control, radiation sterilization technology is widely used in the sterilization of bone allografts. During the radiation sterilization of bone allografts, the irradiation dose should be optimized to ensure sterilization of grafts and preservation of biological properties of bone. The immunogenicity of allografts is decreased by irradiation. IAEA devoted great efforts to generalization of the radiation sterilization of tissue allografts in developing countries since 1986. Tissue Bank of China Institute for Radiation Protection (CIRP) was initially established in 1988 with the support of IAEA, afterwards restructured into Shanxi Provincial Tissue Bank (SPTB). The SPTB, as the first manufacturer of the irradiated bone allografts in the country, was granted production license by the State Food and Drug Administration of China. The SPTB sponsored IAEA/RCA Training Courses, National Symposium on Bone Grafting, and National Training Course on Bone Banking. Technique of radiation sterilization for bone grafts has become popularized in China after these activities. (authors)

Determination of the dose of gamma radiation sterilization for assessment of biological parameters of male Ceratitis capitada (Diptera: Tephritidae), tsl - Vienna 8 strain; Determinacao da dose de radiacao gama esterilizante pela avaliacao dos parametros biologicos de machos de Ceratitis capitata (Diptera: Tephritidae), linhagem tsl - Vienna 8

Energy Technology Data Exchange (ETDEWEB)

Rocha, Aline Cristina Pereira da

2011-07-01

The Vienna-8, tsl (temperature sensitive lethal) strain of Ceratitis capitata, by presenting mutations that facilitate the mass rearing and release only of sterile males in the field, has been used in (Sterile Insect Technique) programmes. The objective of this study was to determine the radiation dose that provides the highest level of sterility for Vienna-8, tsl males assessing their biological parameters that indicate the quality of sterile males to be released. Brown pupae (males) of the tsl strain were obtained from the mass rearing of the Food Irradiation and Radio entomology laboratory of CENA/USP, and they were irradiated (with gamma radiation - {sup 60}Co) 24 hours before the emergence at rates of 0, 30, 60, 90 and 120 Gy. The determination of the sterilizing dose was based on fertility of sexually mature females of the bisexual strain and not irradiated, mated with males of different treatments. Eggs were collected daily during 6 days, were counted and it was possible to estimate fecundity, and assess the hatching rate. The emergence and flight ability were determined by following the protocol of quality control manual for FAO/IAEA/USDA (2003). To assess the longevity under nutritional stress, the insects were kept a period of 48 h after emergence in the absence of water and food, and after this period, mortality was recorded. The size of the testes (left and right) was obtained by dissecting irradiated and non-irradiated males at the eighth day of life, and measure the testes in an ocular micrometer, considering the maximum length and width of each sample. To determine the sperm number was necessary to dissect the males and break their testicles. No difference was observed in emergence rate, flight ability and longevity of irradiated and non-irradiated males, nor in the fecundity of females mated with males of different treatments. The sterilizing dose that resulted in lower fertility of females was 120 Gy, with 1.5% hatching. Considering the parameters

Trends in sterilization since the introduction of Essure hysteroscopic sterilization.

Science.gov (United States)

Shavell, Valerie I; Abdallah, Mazen E; Shade, George H; Diamond, Michael P; Berman, Jay M

2009-01-01

To investigate trends in sterilization in women at the Detroit Medical Center, Michigan (DMC), since the introduction of Essure hysteroscopic sterilization. Retrospective study (Canadian Task Force classification II-2). Outpatient surgery center and university teaching hospitals. Women who underwent interval sterilization procedures at the DMC (Hutzel Women's Hospital, Sinai-Grace Hospital, and the Berry Center) and postpartum sterilization procedures at Hutzel Women's Hospital between January 1, 2002, and December 31, 2007. Permanent sterilization procedures including minilaparotomy tubal ligation, laparoscopic sterilization, Essure hysteroscopic sterilization, and postpartum tubal ligation performed at the time of cesarean section or after vaginal delivery. In all, 5509 permanent sterilization procedures were performed in the 6 years between January 1, 2002, and December 31, 2007, at the DMC facilities analyzed: 2484 interval sterilization procedures at Hutzel Women's Hospital, Sinai-Grace Hospital, and the Berry Center, and 3025 postpartum tubal ligations at Hutzel Women's Hospital. From 2002 through 2007, the decrease in laparoscopic sterilizations from 97.9% to 48.5% of all interval sterilization procedures corresponded significantly with the increase in Essure hysteroscopic sterilizations from 0.0% to 51.3% (p Essure hysteroscopic sterilizations increased significantly from 0.0% to 51.3% of all procedures. Since the approval of Essure hysteroscopic sterilization in November 2002, this minimally invasive method of hysteroscopic sterilization has increased in popularity at the DMC.

Agrobacterium-mediated transformation of black cherry for flowering control and insect resistance

Science.gov (United States)

Ying Wang; Paula M. Pijut

2014-01-01

Black cherry is one of the most valuable hardwood species for cabinetry, furniture, and veneer. The goal of this study was to develop transgenic black cherry plants with reproductive sterility and enhanced insect resistance. Black cherry TERMINAL FLOWER 1 (PsTFL1) was overexpressed under the control of the CaMV 35S promoter in black cherry via

Sterilization of health care products - Radiation. Part 2: Establishing the sterilization dose

International Nuclear Information System (INIS)

2006-01-01

devised specifically for substantiation of 25 kGy, Method VD max, was put forward by Kowalski and Tallentire (1999) [14]. Subsequent evaluations involving computational techniques demonstrated that the underlying principles were soundly based (Kowalski, Aoshuang and Tallentire, 2000) [13] and field trials confirmed that Method VD max is effective in substantiating 25 kGy for a wide variety of medical devices manufactured and assembled in different ways (Kowalski et al., 2002) [16]. A standardized procedure for the use of VD max for substantiation of a sterilization dose of 25 kGy has been published in the AAMI Technical Information Report Sterilization of health care products -- Radiation sterilization -- Substantiation of 25 kGy as a sterilization dose - Method VD max (AAMI TIR27:2001) [5], a text on which the method described herein is largely based. Method VD max is founded on dose setting Method 1 and, as such, it possesses the high level of conservativeness characteristic of Method 1. In a similar manner to the dose setting methods, it involves performance of tests of sterility on product items that have received a dose of radiation lower than the sterilization dose. The outcomes of these tests are used to substantiate that 25 kGy achieves an SAL of 10 -6 . To link the use of VD max for the substantiation of a particular preselected sterilization dose, the numerical value of the latter, expressed in kGy, is included as a superscript to the VD max symbol. Thus, for substantiation of a sterilization dose of 25 kGy the method is designated VD max 25 . Method VD max 15 is based on the same principles as Method VD max 25 described above. The test procedure is the same as Method VD max 25 , but VD max 15 is limited to product with average bioburden ≤ 1,5. The outcomes of these tests are used to substantiate that 15 kGy achieves a sterility assurance level of 10 -6 . This part of ISO 11137 also describes methods that may be used to carry out sterilization dose audits in

Integrated programme to eradicate the old world screwworm fly from the Middle East

International Nuclear Information System (INIS)

Al-Izzy, M. A. J.; Al-Taweel, A. A.

2011-01-01

The Old World Screwworm fly is one of the most destructive insect pests of livestock, humans and wildlife. It was recognized by World Organization for Animal Health as a notifiable disease. The outbreak of Old World Screwworm in Iraq in the late 1996 led to a joint regional/international meeting under the umbrella of the Arab Organization for Agricultural Development to demonstrate the feasibility of eradicating Old World Screwworm from the gulf region by using the sterile insect technique as an agent of the Area- Wide Integrated Pest Management campaign. The programme approved by Arab Organization for Agricultural Development , Food and Agriculture Organization and International Atomic Energy Agency included identification of different stages of Old World Screwworm, Chrysomya bezziana, increasing the awareness of the animal breeder against the danger of this pest, the possibility of rearing this pest in the laboratory for carrying different studies such as the effect of gamma rays on different insect stages, induced sterility, competitiveness,.. etc. Furthermore, the programme included genetic diversity studies on the Old World Screwworm, Chrysomya bezziana strain which was found in different Arab Gulf countries to demonstrate if this diversity has any effect on using sterile insect technique to eradicate this pest from the gulf region. Finally the programme demonstrated the economic feasibility of using sterile insect technique for eradicating this pest from the gulf countries. (author)

Comparison of Sterile and Clean Dressing Techniques in Post ...

African Journals Online (AJOL)

Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, ... Chinese hospital, and to compare their impact on wound healing and the cost of the ... up to the time of their discharge. Results: Twelve (18.5 %) patients out of those who received sterile or clean .... identified using automated means [13,14].

Insect disinfestation of packed dates by gamma-radiation

International Nuclear Information System (INIS)

Ahmed, M.S.H.; Hameed, A.A.; Kadhum, A.A.; Ali, S.R.

1985-01-01

The effect of gamma-radiation on insect disinfestation of commercially packed dry dates was studied in collaboration with the Iraqui Date Administration. The wrapping of ''window carton lunch boxes'' (each holding 250 g of dry dates, Zahdi variety) with polyethylene film, compared to cellophane, prevented reinfestation for a long period of storage if treated with 0.70 kGy of gamma-radiation. All live insects that were found in the irradiated boxes and tested were genetically sterile, and those in different developmental stages died within a short period of time without passing through metamorphosis. In all cases (treated or control batches), cellophane-sealed boxes proved to be more vulnerable to reinfestation. Dates packed in plastic cups, used for N/sub 2/ vacuum packaging, were also effectively disinfested by the same dose of radiation. The value of airtight packaging will certainly add to the advantage of the high penetration power of gamma-radiation relative to methyl bromide fumigation. Results of the assessment of the damaging ability of irradiated insects indicate that treatment of packed dates with 0.70 kGy is sufficient as far as quarantine measures are concerned where the possible increase in infestation rate, brought about by radio-resistant insect stages that usually constitute a small fraction in nature, is negligible

Short range attraction of Ceratitis capitata (Diptera: Tephritidae) sterile males to six commercially available plant essential oils

Science.gov (United States)

Plant essential oils have a number of roles in insect pest management. For male Ceratitis capitata, this includes use of angelica seed oil as long range attractants and ginger root oil as aromatherapy, which is exposure to sterile males to increase mating success. Neither of these plants are hosts f...

Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka.

Science.gov (United States)

Gunathilaka, Nayana; Ranathunge, Tharaka; Udayanga, Lahiru; Abeyewickreme, Wimaladharma

2017-01-01

Selection of the artificial membrane feeding technique and blood meal source has been recognized as key considerations in mass rearing of vectors. Artificial membrane feeding techniques, namely, glass plate, metal plate, and Hemotek membrane feeding method, and three blood sources (human, cattle, and chicken) were evaluated based on feeding rates, fecundity, and hatching rates of Aedes aegypti . Significance in the variations among blood feeding was investigated by one-way ANOVA, cluster analysis of variance (ANOSIM), and principal coordinates (PCO) analysis. Feeding rates of Ae. aegypti significantly differed among the membrane feeding techniques as suggested by one-way ANOVA ( p feeding technique. Blood feeding rate of Ae. aegypti was higher with human blood followed by cattle and chicken blood, respectively. However, no significant difference was observed from the mosquitoes fed with cattle and human blood, in terms of fecundity, oviposition rate, and fertility as suggested by one-way ANOVA ( p > 0.05). Metal plate method could be recommended as the most effective membrane feeding technique for mass rearing of Ae. aegypti , due to its high feeding rate and cost effectiveness. Cattle blood could be recommended for mass rearing Ae. aegypti .

Periodontal instrumentation for the general dental practitioner: Pt. 2. Management maintenance and sterilization

Energy Technology Data Exchange (ETDEWEB)

Touyz, L.Z.G. (University of the Witwatersrand, Johannesburg (South Africa). Dept. of Oral Medicine and Periodontology); De Waal, J.

1983-03-01

The purpose of this article is to describe the management, maintenance and sterilization of periodontal instrumentation used by the general dental practitioner. The pre-sterilization, decontamination and cleaning, the sharpening of instrumentation and the packaging, identification and grouping for sterilization are discussed. Attention is also given to various techniques of sterilization, including gamma radiation.

Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka

Directory of Open Access Journals (Sweden)

Nayana Gunathilaka

2017-01-01

Full Text Available Introduction. Selection of the artificial membrane feeding technique and blood meal source has been recognized as key considerations in mass rearing of vectors. Methodology. Artificial membrane feeding techniques, namely, glass plate, metal plate, and Hemotek membrane feeding method, and three blood sources (human, cattle, and chicken were evaluated based on feeding rates, fecundity, and hatching rates of Aedes aegypti. Significance in the variations among blood feeding was investigated by one-way ANOVA, cluster analysis of variance (ANOSIM, and principal coordinates (PCO analysis. Results. Feeding rates of Ae. aegypti significantly differed among the membrane feeding techniques as suggested by one-way ANOVA (p0.05. Conclusions. Metal plate method could be recommended as the most effective membrane feeding technique for mass rearing of Ae. aegypti, due to its high feeding rate and cost effectiveness. Cattle blood could be recommended for mass rearing Ae. aegypti.

Use of Nuclear Techniques in Biological Control: Managing Pests, Facilitating Trade and Protecting the Environment. Report of a Consultants Group Meeting. Working Material

International Nuclear Information System (INIS)

1999-01-01

High-priority opportunities are proposed for use of nuclear techniques to effect improved production and shipping of augmentative biological control agents. Proposed subprojects include use of ionizing radiation to improve the production of insect natural enemies on natural hosts/prey or on artificial diets. Other subprojects pertain to improving the ability to move beneficial organisms in international trade, and in using them in the field. Additional high priority activities were identified proposing use of nuclear techniques to produce sterile and/or substerile F-1 weed biological control agents to help evaluate potential impact on non-target species in the pre-release phase, integration of augmentative releases and F-1 sterility in IPM and area-wide pest management programmes, and utilization of by-products from SIT mass-rearing facilities in augmentative biological control programmes. (author)

The study of insect blood-feeding behaviour: 2. Recording techniques and the use of flow charts

Directory of Open Access Journals (Sweden)

J. J. B. Smith

1987-01-01

Full Text Available This paper continues a discussion of approaches and methodologies we have used in our studies of feeding in haematophagous insects. Described are techniques for directly monitoring behaviour: electrical recording of feeding behaviour via resistance changes in the food canal, optical methods for monitoring mouthpart activity, and a computer technique for behavioural event recording. Also described is the use of "flow charts" or "decision diagrams" to model interrelated sequences of behaviours.

The influence of sterilization on nitrogen-included ultrananocrystalline diamond for biomedical applications.

Science.gov (United States)

Tong, Wei; Tran, Phong A; Turnley, Ann M; Aramesh, Morteza; Prawer, Steven; Brandt, Milan; Fox, Kate

2016-04-01

Diamond has shown great potential in different biomedical applications, but the effects of sterilization on its properties have not been investigated. Here, we studied the influence of five sterilization techniques (solvent cleaning, oxygen plasma, UV irradiation, autoclave and hydrogen peroxide) on nitrogen-included ultrananocrystalline diamond. The chemical modification of the diamond surface was evaluated using X-ray photoelectron spectroscopy and water contact angle measurements. Different degrees of surface oxidation and selective sp(2) bonded carbon etching were found following all sterilization techniques, resulting in an increase of hydrophilicity. Higher viabilities of in vitro mouse 3T3 fibroblasts and rat cortical neuron cells were observed on oxygen plasma, autoclave and hydrogen peroxide sterilized diamond, which correlated with their higher hydrophilicity. By examination of apatite formation in simulated body fluid, in vivo bioactivity was predicted to be best on those surfaces which have been oxygen plasma treated and lowest on those which have been exposed to UV irradiation. The charge injection properties were also altered by the sterilization process and there appears to be a correlation between these changes and the degree of oxygen termination of the surface. We find that the modification brought by autoclave, oxygen plasma and hydrogen peroxide were most consistent with the use of N-UNCD in biological applications as compared to samples sterilized by solvent cleaning or UV exposure or indeed non-sterilized. A two-step process of sterilization by hydrogen peroxide following oxygen plasma treatment was then suggested. However, the final choice of sterilization technique will depend on the intended end application. Copyright © 2015 Elsevier B.V. All rights reserved.

Female sterilization by tubal ligation at caesarean section in Makurdi ...

African Journals Online (AJOL)

Background: Female sterilization is an important tool in reducing unplanned pregnancy and maternal mortality in our environment. The aim of this study was to determine the incidence, sociodemographic characteristics, technique, effectiveness and complications associated with female sterilization by bilateral tubal ligation ...

Insect pests management of bt cotton through the manipulation of different eco-friendly techniques

International Nuclear Information System (INIS)

Ahmad, N.; Khan, M.H.; Tofique, M.

2011-01-01

This study was designed to manage insect pests of Bt cotton through the manipulation of different eco-friendly techniques. A perusal of data, based on the overall performance of different treatments reflected that lowest population of jassids (0.29) was observed in bio-control treated Bt cotton followed by bio-control treated conventional cotton (0.41). Mean per leaf population of thrips was found lowest in insecticide treated Bt cotton (0.97) which was statically at par with bi-control treated conventional cotton (0.95), biocontrol treated Bt cotton (1.09) and colour traps treated Bt cotton (1.50). In case of white flies, bio-control treated Bt cotton and bio-control treated conventional cotton again proved effective in maintaining the population at lower levels per leaf (0.33 and 0.35 respectively). No bollworms infestation was recorded in transgenic cotton whereas higher attack of the same was observed in the untreated conventional cotton block. The best results were achieved with the application of bio-control agents in combination with Bt cotton resulting in least infestation by insect pests and maximum seed yield of 3657 kg/ha. The population of Chrysoperla carnea was significantly higher in Bt and conventional cotton treated with bio-control agents as compared to the other treatments. The parasitism percentage of Trichogramma chilonis was observed significantly higher in bio-control treated conventional cotton. The studies manifested that combination of bio-control technology with Bt cotton effectively preserves the local beneficial insect fauna indicating its potential to be used as integrated management system against different insect pests of cotton. (author)

Effects of pre-irradiation conditioning of Medfly pupae (Diptera: Tephritidae): Hypoxia and quality of sterile males

International Nuclear Information System (INIS)

Nestel, D.; Nemny-Lavy, E.; Islam, S.M.; Wornoayporn, V.; Caceres, C.

2007-01-01

Irradiation of pupae in sterile insect technique (SIT) projects is usually undertaken in hypoxic atmospheres, which have been shown to lessen the deleterious effects of irradiation on the quality of adult sterile flies. Although this is the accepted technology in most mass-rearing and sterilization facilities, to date no information has been generated on the actual levels of oxygen (O 2 ) in pupae-packing containers during irradiation. The present study utilized recently-developed technology to investigate the O 2 level inside bags in which pupae of Mediterranean fruit fly (medfly) Ceratitis capitata (Wiedemann) are packed prior to irradiation, the ability of pupae to create hypoxic environments in these bags, and the effect of O 2 atmospheres on the quality of irradiated males. Pupae, 1 d before adult emergence, were shown to deplete the O 2 level in sealed bags in approximately 1 h. The rate of O 2 consumption was dependent upon pupal age and incubation temperature. Incubation temperature did not significantly affect the quality of pupae or mating capacity of resultant adult males if pupae were irradiated under maximal hypoxic conditions inside packing bags. In contrast, mating competitiveness drastically decreased when pupae were irradiated under ambient O 2 conditions, with the packing bag open. There was no difference in the mating capacity of males when pupae were irradiated in sealed bags under either 10% or 2% O 2 levels, or under maximal hypoxia. Normal doses of fluorescent dye, applied to pupae to mark sterile flies, did not affect the ability of pupae to create hypoxic conditions inside packing bags, nor the quality control parameters of either pupae or adults. Current practices in mass-rearing facilities are discussed in the light of these results. (author) [es

Integrated pest management

International Nuclear Information System (INIS)

LaBrecque, G.C.

1981-01-01

An effective Integrated Pest Management (IPM) programme requires a thorough knowledge of the biology of the target species, namely information on the dispersal, population densities and dynamics as well as the ecology of the natural enemies of the pest. Studies on these can be accomplished by radiolabelling techniques. In the event that conditions prevent the use of radioisotopes the insects can be labelled with either a rare earth or stable isotopes. All insects treated with the rare earths, once captured, are exposed to neutrons which produce radioactivity in the rare earths. There are two other approaches in the practical application of radiation to the problem of insect control: the exposure of insects to lethal doses of radiation and the release of sterile insects. The Insect and Pest Control Section contributes to all aspects of the sterile insect technique (SIT) and it is involved in the Agency's Coordinated Research Programme which permits scientists from the developing countries to meet to discuss agricultural problems and to devise means of solving crop-pest infestation problems by using isotopes and radiation. The success of radiation in insect pest control was underlined and reviewed at the international symposium on the sterile insect technique and the use of radiation in genetic insect control jointly organized by the FAO and the IAEA and held in the FRG in 1981. Another important action is the BICOT programme in Nigeria between the IAEA and the Government of Nigeria on the biological control of tsetse flies by SIT

Process variation in electron beam sterilization

International Nuclear Information System (INIS)

Beck, Jeffrey A.

2012-01-01

The qualification and control of electron beam sterilization can be improved by the application of proven statistical analysis techniques such as Analysis of Variance (ANOVA) and Statistical Tolerance Limits. These statistical techniques can be useful tools in: •Locating and quantifying the minimum and maximum absorbed dose in a product. •Estimating the expected process maximum dose, given a minimum sterilizing dose. •Setting a process minimum dose target, based on an allowance for random measurement and process variation. •Determining the dose relationship between a reference dosimeter and process minimum and maximum doses. This study investigates and demonstrates the application of these tools in qualifying electron beam sterilization, and compares the conclusions obtained with those obtained using practices recommended in Guide for Process Control in Radiation Sterilization. The study supports the following conclusions for electron beam processes: 1.ANOVA is a more effective tool for evaluating the equivalency of absorbed doses than methods suggested in . 2.Process limits computed using statistical tolerance limits more accurately reflect actual process variability than the AAMI method, which applies +/−2 sample standard deviations (s) regardless of sample size. 3.The use of reference dose ratios lends itself to qualification using statistical tolerance limits. The current AAMI recommended approach may result in an overly optimistic estimate of the reference dose adjustment factor, as it is based on application of +/−2(s) tolerances regardless of sample size.

Evaluation of radiation sensitivity and mating performance of Glossina brevipalpis males.

Directory of Open Access Journals (Sweden)

Chantel J de Beer

2017-03-01

Full Text Available Area-wide integrated pest management strategies that include a sterile insect technique component have been successfully used to eradicate tsetse fly populations in the past. To ensure the success of the sterile insect technique, the released males must be adequately sterile and be able to compete with their native counterparts in the wild.In the present study the radiation sensitivity of colonised Glossina brevipalpis Newstead (Diptera; Glossinidae males, treated either as adults or pupae, was assessed. The mating performance of the irradiated G. brevipalpis males was assessed in walk-in field cages. Glossina brevipalpis adults and pupae were highly sensitive to irradiation, and a dose of 40 Gy and 80 Gy induced 93% and 99% sterility respectively in untreated females that mated with males irradiated as adults. When 37 to 41 day old pupae were exposed to a dose of 40 Gy, more than 97% sterility was induced in untreated females that mated with males derived from irradiated pupae. Males treated as adults with a dose up to 80 Gy were able to compete successfully with untreated fertile males for untreated females in walk-in field cages.The data emanating from this field cage study indicates that, sterile male flies derived from the colony of G. brevipalpis maintained at the Agricultural Research Council-Onderstepoort Veterinary Institute in South Africa are potential good candidates for a campaign that includes a sterile insect technique component. This would need to be confirmed by open field studies.

Sterilization by cobalt 60 of the mediterranean fruit fly Ceratitis Capitate Wied

International Nuclear Information System (INIS)

Dimundu Puena, I.; Cirio, Ugo.

1978-01-01

Ceratitis capitata have been irradiated with 2, 4, 6 and 10 Krad of a Co-60 gamma irradiator at 300 rad/min flow. Following results have been obtained: abundant and regular egg laid at 1 to 2 krads, reduced but regular egg laid at 4 krads and total sterility from 6 krads. The 10 krads irradiated insects exhibit satisfactory life time and activity. The 6 and above krads irradiated females are completely sterile but have the laid reflex. The 8 krads irradiated males exhibit sexual strength comparable to that of normal males. Males fertility is decreased with increasing irradiation dose, eggs obtained with normal female have fertility of less than 1 % for doses above 7 krads. However it appears that at 8 krads dose, some males can form again normal spermatozoids after 20 to 30 days. The recommended irradiation dose is 10 krads

Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka

OpenAIRE

Nayana Gunathilaka; Tharaka Ranathunge; Lahiru Udayanga; Wimaladharma Abeyewickreme

2017-01-01

Introduction Selection of the artificial membrane feeding technique and blood meal source has been recognized as key considerations in mass rearing of vectors. Methodology Artificial membrane feeding techniques, namely, glass plate, metal plate, and Hemotek membrane feeding method, and three blood sources (human, cattle, and chicken) were evaluated based on feeding rates, fecundity, and hatching rates of Aedes aegypti. Significance in the variations among blood feeding was investigated by one...

Comparative rearing parameters for bisexual and genetic sexing strains of Zeugodacus cucurbitae and Bactrocera dorsalis (Diptera: Tephritidae) on an artificial diet

Science.gov (United States)

The Sterile Insect Technique (SIT) is an important component of area wide programs to control invading or established populations of pestiferous tephritids. The SIT involves the production, sterilization, and release of large numbers of the target species, with the goal of obtaining sterile male x w...

Radiation sterilization of hydrocortisone acetate

International Nuclear Information System (INIS)

Charef, A.; Boussaha, A.

1989-09-01

The feasibility of using high energy ionizing radiation for the sterilization of hydrocortisone acetate was investigated. Hydrocortisone acetate in the form of powder was exposed to different dose levels of gamma radiation using a Cobalt-60 source. The irradiated samples were examined by various physico-chemical techniques in order to detect possible radiolysis products. It was of interest to know if one could insure sterility and retain biological properties of the drug by suitable choice of radiation dose. The results showed that a 10 KGy radiation dose causes no change in the physico-chemical properties of the drug and is sufficient to obtain contaminant-free product

Radiation sterilization

International Nuclear Information System (INIS)

Jacobs, G.P.

1989-01-01

In view of the application of ionizing radiation to sterilize pharmaceutical products, and the particular advantages of using this mode of sterilization for powders for injection, which cannot be sterilized by more conventional methods, it is important to recognise the possibility of modification of radiation response of bacteria when in close contact with various drug powders. For this study, bacterial spores, which lend themselves to dessication, and which can be dried onto an inert powder matrix, were chosen as the test system. The results of this work indicate that the additives tested have a modest protective effect on the spores. However, when considering a bacterial inactivation for sterilization purposes of between six and ten orders of magnitude, that is, a desired sterility assurance level of an expected maximum probability of a product item being non-sterile of 10 -6 , then the slight protective effect observed in this study approaches insignificance

Integrated management of fruit flies

International Nuclear Information System (INIS)

1983-01-01

This film introduces species of fruit-flies and their reproduction cycle and suggests various methods for controlling insect pests (insect traps, treatment of infested fruits, chemical, legal, and biological control -sterile male technique

Laparoscopic tubal sterilization reversal and fertility outcomes

Directory of Open Access Journals (Sweden)

K Jayakrishnan

2011-01-01

Full Text Available Purpose: The purpose of the study was two-fold. Firstly it was to assess the suitability for tubal recanalization and factors predicting successful laparoscopic recanalization. Secondly, it was to analyze the fertility outcomes and factors affecting the pregnancy rate following laparoscopic tubal recanalization. Materials and Methods: A retrospective chart review of prospectively followed-up 29 women at a tertiary care center seeking tubal sterilization reversal between May 2005 and February 2010 were included. Results: In 14 (48.3% women unilateral tubes were suitable and in only 3 women (10.3% bilateral tubes were suitable. All cases with laparoscopic tubal sterilization were suitable, whereas all cases with fimbriectomy were unsuitable for recanalization. In 6 (20.7% cases salphingostomy was performed as an alternative procedure to tubal reanastomosis. The overall pregnancy rate was 58.8%. In cases with sterilization by Pomeroy′s method, 4 out of 10 (40% conceived, whereas for laparoscopic tubal ligation cases 6 out of 7 (85.7% conceived (P=0.32. None of the patients with final tubal length <5 cm conceived (P=0.03. Comparing the age at recanalization, in women ≤30 years, 71.4% conceived, as compared with 50% when age of women was more than 30 years (P=0.37. Conclusions: The important factors determining the success of recanalization are technique of sterilization and the remaining length of the tube after recanalization. The gynecologist must use an effective technique of sterilization to minimize the failure rates, but at the same time, which causes minimal trauma, and aim at preserving the length of the tube so that reversal is more likely to be successful, should the patient′s circumstances change.

Insect Capital

Directory of Open Access Journals (Sweden)

Andrew Pilsch

2015-12-01

Full Text Available In this note, Pilsch address William Gibson’s use of insect imagery in to trouble the common understanding of the novel Neuromancer, its commentary on corporate culture, and its relationship to a then-emergent posthumanism. Further, he concludes by suggesting that, for Gibson, the insect hive as an image for the corporate body shows that corporate culture is, in contrast to the banal image the term brings to mind, a set of nefarious cultural techniques derived for interfacing human bodies with the corporation’s native environment in the postmodern era: the abstractions of data.

Sterile neutrino

International Nuclear Information System (INIS)

Anon.

2007-01-01

Paper deals with the information on the occurrence of the fields of the sterile neutrinos (the righthanded ones) mixed with the normal neutrinos (the lefthanded ones). Both the Max Plank Radioastronomy Institute and the Los Angeles University assumes that the occurrence of the keV mass sterile neutrinos may explain the dark matter nature, the fast rotation of the observed pulsars and the reionization processes. The issues associated with the possibility to record the sterile neutrinos were analyzed in the course of the Sterile Neutrinos in Astrophysics and Cosmology Workshop (Crans Montana, March 2006 [ru

Long distance transport of irradiated male Glossina palpalis gambiensis pupae and its impact on sterile male yield

International Nuclear Information System (INIS)

Pagabeleguem, Soumaila; Seck, Momar Talla; Sall, Baba

2015-01-01

The application of the sterile insect technique (SIT) requires mass-production of sterile males of good biological quality. The size of the project area will in most cases determine whether it is more cost effective to produce the sterile flies locally (and invest in a mass-rearing facility) or import the sterile flies from a mass-rearing facility that is located in another country. This study aimed at assessing the effect of long distance transport of sterile male Glossina palpalis gambiensis pupae on adult male fly yield. The male pupae were produced at the Centre International de Recherche-Developpement sur l'Elevage en zone Subhumide (CIRDES), Bobo-Dioulasso, Burkina Faso, and shipped with a commercial courier service in insulated transport boxes at a temperature of ±10° to Senegal (36 h of transport). Upon arrival in the insectary in Dakar, the pupae were transferred to an emergence room and the flies monitored for 3-6 days. The results showed that the used system of isothermal boxes that contained phase change material packs (S8) managed to keep the temperature at around 10° which prevented male fly emergence during transport. The emergence rate was significantly higher for pupae from batch 2 (chilled at 4° for one day in the source insectary before transport) than those from batch 1 (chilled at 4° for two days in the source insectary before transport) i.e. an average (±sd) of 76.1 ± 13.2% and 72.2 ± 14.3%, respectively with a small proportion emerging during transport (0.7 ± 1.7% and 0.9 ± 2.9%, respectively). Among the emerged flies, the percentage with deformed (not fully expanded) wings was significantly higher for flies from batch 1 (12.0 ± 6.3%) than from batch 2 (10.7 ± 7.5%). The amount of sterile males available for release as a proportion of the total pupae shipped was 65.8 ± 13.3% and 61.7 ± 14.7% for batch 1 and 2 pupae, respectively. The results also showed that the temperature inside the parcel must be controlled around 10° with a

Gamma-sterilization

International Nuclear Information System (INIS)

Lindgren, E.

1974-01-01

The author makes a survey of his experience in sterilization and sterility control of medical products. At present three different methods are used, steamsterilization, gassterilizing and gammasterilizing. The investments and costs for gamma radiation is presented and a comparison of the costs for gamma- and gassterilization including sterility control is made. (M.S.)

Sterile Neutrino Search in the NOvA Far Detector

Energy Technology Data Exchange (ETDEWEB)

Edayath, Sijith [Cochin U.; Aurisano, Adam [Cincinnati U.; Sousa, Alexandre [Cincinnati U.; Davies, Gavin [Indiana U.; Suter, Louise [Fermilab; Yang, Shaokai [Cincinnati U.

2017-10-03

The majority of neutrino oscillation experiments have obtained evidence for neutrino oscillations that are compatible with the three-flavor model. Explaining anomalous results from short-baseline experiments, such as LSND and MiniBooNE, in terms of neutrino oscillations requires the existence of sterile neutrinos. The search for sterile neutrino mixing conducted in NOvA uses a long baseline of 810 km between Near Detector (ND) at Fermilab and Far Detector (FD) in Minnesota. The signal for sterile neutrino oscillations is a deficit of neutral-current neutrino interactions at the FD with respect to the ND prediction. In this document, We will present the analysis improvements that we are implementing for future NC sterile neutrino searches with NOvA. These include: improved modelling of our detector response; the inclusion of NC 2p2h interaction modelling; implementing a better energy reconstruction techniques; and including possible oscillation due to sterile neutrinos in the ND . This improvements enable us to do a simultaneous ND-FD shape fit of the NC energy spectrum covering a wider sterile mass range than previous analyses.

Effect of sterilization procedures on properties of plasma polymers relevant to biomedical applications

International Nuclear Information System (INIS)

Artemenko, A.; Kylián, O.; Choukourov, A.; Gordeev, I.; Petr, M.; Vandrovcová, M.; Polonskyi, O.; Bačáková, L.; Slavinska, D.; Biederman, H.

2012-01-01

This study is focused on the evaluation of resistance of plasma polymers toward common sterilization techniques, i.e. property important for possible use of such materials in biomedical applications. Three kinds of plasma polymers having different bioadhesive natures were studied: plasma polymerized poly(ethylene oxide), fluorocarbon plasma polymers, and nitrogen-rich plasma polymers. These plasma polymers were subjected to dry heat, autoclave and UV radiation treatment. Their physical, chemical and bioresponsive properties were determined by means of different techniques (ellipsometry, atomic force microscopy, wettability measurements, X-ray photoelectron spectroscopy and biological tests with osteoblast-like cells MG63). The results clearly show that properties of thin films of plasma polymers may be significantly altered by a sterilization process. Moreover, observed changes induced by selected sterilization methods were found to depend strongly on the sterilized plasma polymer. - Highlights: ► Effect of common sterilization methods on three kinds of plasma polymers is studied. ► Physical, chemical and bioresponsive properties of plasma polymers are analyzed. ► Changes induced by sterilization depend strongly on type of the plasma polymer.

Microbiological method for radiation sterilization (I). General knowledge and handling technique for bacterial identification

International Nuclear Information System (INIS)

Koshikawa, Tomihiko

2004-01-01

The part I in this title series describes the basic knowledge and technique for identification of bacteria in the radiation sterilization of medical devices, where the radiation dose can be decided from the number and radio-resistance of the bioburden (bacteria on the device). Four essential, actual technologies for identification are described: isolation and storage of bacteria; decision of bacterial natures, involving 3 Gram staining methods, morphology by microscopy and/or phase-contrast microscopy, spore-forming bacteria, and size measurement by micrometry; Other test items for identification of genus, involving motility, oxygen demand, catalase, oxidase, acid production from glucose, and OF (oxidation or fermentation for glucose degradation) test; and colony observation. Media, identification kits and record forms for these are presented. (N.I.)

Research on industrial development of radiation sterilization in Sichuan

International Nuclear Information System (INIS)

Chen Xun; Zhu Rong; Chen Jigang; Ouyang Qiongli

2010-01-01

Radiation sterilization is widely applied in food processing, pharmaceutical manufacturing and medical instrumentation producing. As this industry offers service to related corporations, its development depends largely on the market scale of the relevant industries. This article mainly discusses the balance point between the capacity of radiation sterilization and the requirement of its market. There are less than 1% of the companies in Sichuan which using radiation sterilization for its products, broad prospects has shown in this field. There are 3 key points to make its output value reach 0.1% of the GDP in Sichuan: 1. Breaking the restrictions of capacity and inappropriate resources distribution. 2. Promoting the technique of radiation sterilization, guiding corporation to reduce their dependence on food additive from the perspective of food safety, make effective implantation of radiation sterilization into their manufacture processes. 3. Increasing publicity, eliminating scared mentality among customer on nuclear technology, helping manufacturing corporations to establish green environmental protection brand, directing our customer to a more healthy awareness. (authors)

Effects of sterilization treatments on bulk and surface properties of nanocomposite biomaterials.

Science.gov (United States)

Ahmed, Maqsood; Punshon, Geoffrey; Darbyshire, Arnold; Seifalian, Alexander M

2013-10-01

With the continuous and expanding use of implantable biomaterials in a clinical setting, this study aims to elucidate the influence of sterilization techniques on the material surface and bulk properties of two polyurethane nanocomposite biomaterials. Both solid samples and porous membranes of nondegradable polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) and a biodegradable poly(caprolactone-urea) urethane (POSS-PCL) were examined. Sterilization techniques included conventional steam sterilization (autoclaving), gamma irradiation, and disinfection via incubating with ethanol (EtOH) for 10 min or 24 h. After treatment, the samples were examined using gel permeation chromatography (GPC), attenuated total reflectance Fourier transform infrared spectroscopy, and tensiometry. Cytotoxicity was evaluated through the culture of endothelial progenitor cells and the efficacy of sterilization method was determined by incubating each sample in tryptone soya broth and fluid thioglycollate medium for cultivation of microorganisms. Although EtOH did not affect the material properties in any form, the samples were found to be nonsterile with microbial growth detected on each of the samples. Gamma irradiation was not only effective in sterilizing both POSS-PCU and POSS-PCL but also led to minor material degradation and displayed a cytotoxic effect on the cultured cells. Autoclaving was found to be the optimal sterilization technique for both solid and porous membranes of the nondegradable POSS-PCU samples as it was successful in sterilizing the samples, displayed no cytotoxic side effects and did not degrade the material. However, the biodegradable POSS-PCL was not able to withstand the harsh environment during autoclaving, resulting in it losing all structural integrity. Copyright © 2013 Wiley Periodicals, Inc., a Wiley Company.

ISO radiation sterilization standards

International Nuclear Information System (INIS)

Lambert, Byron J.; Hansen, Joyce M.

1998-01-01

This presentation provides an overview of the current status of the ISO radiation sterilization standards. The ISO standards are voluntary standards which detail both the validation and routine control of the sterilization process. ISO 11137 was approved in 1994 and published in 1995. When reviewing the standard you will note that less than 20% of the standard is devoted to requirements and the remainder is guidance on how to comply with the requirements. Future standards developments in radiation sterilization are being focused on providing additional guidance. The guidance that is currently provided in informative annexes of ISO 11137 includes: device/packaging materials, dose setting methods, and dosimeters and dose measurement, currently, there are four Technical Reports being developed to provide additional guidance: 1. AAMI Draft TIR, 'Radiation Sterilization Material Qualification' 2. ISO TR 13409-1996, 'Sterilization of health care products - Radiation sterilization - Substantiation of 25 kGy as a sterilization dose for small or infrequent production batches' 3. ISO Draft TR, 'Sterilization of health care products - Radiation sterilization Selection of a sterilization dose for a single production batch' 4. ISO Draft TR, 'Sterilization of health care products - Radiation sterilization-Product Families, Plans for Sampling and Frequency of Dose Audits'

Radio-sterilization and processing of frozen human skin

International Nuclear Information System (INIS)

Zarate S, Herman; Aguirre H, Paulina; Silva R, Samy; Hitschfeld G, Mario

2006-01-01

The Laboratory of Radio-sterilized Biological Tissues Processing (LPTR) belonging to the Chilean Commission of Nuclear Energy and the International Atomic Energy Agency have played a paramount role in our country, concerning the biological tissue processing, which can be radio-sterilized as human skin, pig skin, amniotic membrane, human bone and bovine bone. The frozen radio.-sterilized human skin processing began in 2001, by means of putting into practice the knowledge acquired in training courses through the IAEA and the experience transferred by experts who visited our laboratory. The human skin processing of dead donor can be divided into 6 stages: a) Profuse washing with physiological sterilized serum in to remove the microorganisms, chemical and pharmacological compounds; b) immersion in glycerol solution at 10% to better keep the stored tissues; c) packing, to avoid post manipulation of the sterilized tissue; d) microbiological controls which allow and guarantee a sterility assurance level of 10 6 ; e) radio-sterilization, technique that consists of exposing the grafts to electromagnetic gamma waves which eliminate the microorganisms of the tissue, f) and finally, dispatching and liberation of the frozen sterilized human skin for its clinical use in different centers that take care of burned people. The LPTR receives feedback from surgeons who have used these tissues in order to improve the processing stages based in an integral quality system ISO 9001.2000. The State Health System in our country counts on limited and scarce resources to implement synthetic substitutes that is why It is considered necessary to spread the use of these noble tissues which have sterility assurance and they are processed at low price

Breeding and maintaining high-quality insects

DEFF Research Database (Denmark)

Jensen, Kim; Kristensen, Torsten Nygård; Heckmann, Lars-Henrik

2017-01-01

Insects have a large potential for sustainably enhancing global food and feed production, and commercial insect production is a rising industry of high economic value. Insects suitable for production typically have fast growth, short generation time, efficient nutrient utilization, high...... reproductive potential, and thrive at high density. Insects may cost-efficiently convert agricultural and industrial food by-products into valuable protein once the technology is finetuned. However, since insect mass production is a new industry, the technology needed to efficiently farm these animals is still...... in a starting phase. Here, we discuss the challenges and precautions that need to be considered when breeding and maintaining high-quality insect populations for food and feed. This involves techniques typically used in domestic animal breeding programs including maintaining genetically healthy populations...

The IAEA Laboratories at Seibersdorf

International Nuclear Information System (INIS)

1987-01-01

The film shows the history, development and activities at the IAEA's Laboratory in Seibersdorf. Recent developments in plant breeding and insect pest control (sterile insect technique) and training facilities for fellows from member states are presented

Reason behind wet pack after steam sterilization and its consequences: An overview from Central Sterile Supply Department of a cancer center in eastern India.

Science.gov (United States)

Basu, Debabrata

Wet pack after steam sterilization process that means there are surely obtain millions of microorganisms that can breed and multiply rapidly and objects are unsterile and can never be used for further procedure. There are many reasons behind the wet pack occurrences after autoclaving like poor quality of wrapping materials, faulty valves of rigid container, faulty loading and packaging technique, poor steam quality, sterilizer malfunction and may be design related problems in CSSD sterile storage area. Cause of wet pack after steam sterilization processes may occur severe problems because of wasted time and effort, increased work load, increased cost, potentially contaminated instruments, infection risk to the patient, poor patient outcomes and delayed or cancellation of procedures. But such wet pack scenario can be avoided by various methods by using good steam (water) quality, performing periodic maintenance of the Autoclaves, avoidance of sterilizer overloading, allowing adequate post sterilization time to cool down the materials to room temperature, using good quality wrapping materials, properly maintain temperature and humidity of sterile storage area etc. Copyright © 2016 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Ltd. All rights reserved.

Sterilization of Lung Matrices by Supercritical Carbon Dioxide.

Science.gov (United States)

Balestrini, Jenna L; Liu, Angela; Gard, Ashley L; Huie, Janet; Blatt, Kelly M S; Schwan, Jonas; Zhao, Liping; Broekelmann, Tom J; Mecham, Robert P; Wilcox, Elise C; Niklason, Laura E

2016-03-01

Lung engineering is a potential alternative to transplantation for patients with end-stage pulmonary failure. Two challenges critical to the successful development of an engineered lung developed from a decellularized scaffold include (i) the suppression of resident infectious bioburden in the lung matrix, and (ii) the ability to sterilize decellularized tissues while preserving the essential biological and mechanical features intact. To date, the majority of lungs are sterilized using high concentrations of peracetic acid (PAA) resulting in extracellular matrix (ECM) depletion. These mechanically altered tissues have little to no storage potential. In this study, we report a sterilizing technique using supercritical carbon dioxide (ScCO2) that can achieve a sterility assurance level 10(-6) in decellularized lung matrix. The effects of ScCO2 treatment on the histological, mechanical, and biochemical properties of the sterile decellularized lung were evaluated and compared with those of freshly decellularized lung matrix and with PAA-treated acellular lung. Exposure of the decellularized tissue to ScCO2 did not significantly alter tissue architecture, ECM content or organization (glycosaminoglycans, elastin, collagen, and laminin), observations of cell engraftment, or mechanical integrity of the tissue. Furthermore, these attributes of lung matrix did not change after 6 months in sterile buffer following sterilization with ScCO2, indicating that ScCO2 produces a matrix that is stable during storage. The current study's results indicate that ScCO2 can be used to sterilize acellular lung tissue while simultaneously preserving key biological components required for the function of the scaffold for regenerative medicine purposes.

Exploring miniature insect brains using micro-CT scanning techniques

Science.gov (United States)

Smith, Dylan B.; Bernhardt, Galina; Raine, Nigel E.; Abel, Richard L.; Sykes, Dan; Ahmed, Farah; Pedroso, Inti; Gill, Richard J.

2016-01-01

The capacity to explore soft tissue structures in detail is important in understanding animal physiology and how this determines features such as movement, behaviour and the impact of trauma on regular function. Here we use advances in micro-computed tomography (micro-CT) technology to explore the brain of an important insect pollinator and model organism, the bumblebee (Bombus terrestris). Here we present a method for accurate imaging and exploration of insect brains that keeps brain tissue free from trauma and in its natural stereo-geometry, and showcase our 3D reconstructions and analyses of 19 individual brains at high resolution. Development of this protocol allows relatively rapid and cost effective brain reconstructions, making it an accessible methodology to the wider scientific community. The protocol describes the necessary steps for sample preparation, tissue staining, micro-CT scanning and 3D reconstruction, followed by a method for image analysis using the freeware SPIERS. These image analysis methods describe how to virtually extract key composite structures from the insect brain, and we demonstrate the application and precision of this method by calculating structural volumes and investigating the allometric relationships between bumblebee brain structures. PMID:26908205

Studies on the ecology of insects sterilized artificially (gamma radiation), 8. Experimental analysis of the sexual competitiveness between normal males of the oriental fruit fly and males sterilized by the /sup 137/Cs gamma radiation

Energy Technology Data Exchange (ETDEWEB)

Kiyoku, M; Tsukuda, R [Okayama Univ. (Japan). Faculty of Agriculture

1974-09-01

Gamma-ray irradiation of 7 KR from /sup 137/Cs on the oriental fruit fly pupae two days before emerging did not significantly reduce the percentage of emergence and longevity of adult flies. However, highly sterile adults (more than 98.9%) were found in those combinations, S..gamma.. x N/sup -/, N..gamma.. x S/sup -/ and S..gamma.. x S/sup -/. The result of observation of sperm activity in spermathecae of females mated with sterilized males showed that the sterility is caused by dominant lethalities. The sexual competitiveness between S..gamma.. and N..gamma.. has been examined in experiments of S..gamma.. x N..gamma.. x N/sup -/. (1) The data suggest that the sterility percentage concerning with egg hatchability is proportional to the rate of sterilized males replaced for normal males when that is expressed with a multiple of the ratio of normal males to normal females. Seven such straight lines thus obtained made us possible to find the theoretical expectation of sterility percentage. (2) Relationship between the percent egg hatch observed (y), or that estimated theoretically (Y), and logarithm of ratio, S..gamma../N..gamma.. (x) was showed to be two straight regression equations, y=18.413-11.952 (x - 1.295) and Y=11.890-15.462 (x - 1.295). Ratio of regression coefficients of the equations was 0.772. (3) Correlations between the sterility percentage and ratio, S..gamma../N..gamma.., showed in three convex curves under three degrees of competitiveness (0.265, 0.576 and 1.001). When both the values of S..gamma../N..gamma.. in the mating experiments and the sterility percentages are given, it is possible to obtain degrees of competitiveness using the curves.

Interaction of gamma radiation and temperature on the determination of the sterilizing dose of some stored products pests

International Nuclear Information System (INIS)

Barbosa, A.P.

1976-08-01

The influence of temperature on sterilizing dosages of gamma radiation was studied for Sitophilus zeamais Mots. in corn, Sitophilus oryzae (L.) in rice, Araecerus fasciculatus (DeGeer) in coffee, and Zabrotes subfasciatus (Boh.) in beans. It was found that temperature has a significant influence on the amount of radiation required to sterilize these species. The highest radiation dosages were required at those temperature most nearly optimum for insect development. The minimum radiation doses required for complete sterility of each species at the indicated temperatures were as follows: Sitophilus zeamais Mots. in corn, 8 krad at 24 0 C; Sitophilus oryzae (L.) in rice, 9 krad at 24 0 C; Araecerus fasciculatus (DeGeer) in coffee, 6 krad at 30 0 C; Zabrotes subfasciatus (Boh.) in beans, 9 krad at 24 - 27 0 C. At either higher or lower temperature smaller radiation dosages were required. (author) [pt

Control and Constraint for Low-Income Women Choosing Outpatient Sterilization

Science.gov (United States)

Leyser-Whalen, Ophra; Berenson, Abbey B.

2012-01-01

Little is known about Hispanics and their contraceptive choices in general, with some past studies detailing non-consensual sterilization. This article is based on interviews with a mostly Hispanic sample of 44 women being sterilized at a public clinic in southeast Texas with the Essure device, which entails a new outpatient sterilization procedure. These women cited relationship factors, wanting to better their and their children’s lives, and past reproductive histories as reasons for deciding on sterilization. They specifically chose Essure as a result of an apprehension of surgery and potential side effects from tubal ligation. Their choices, however, were limited by larger structural factors of work, family, the political economy, and the health care system. We conclude that this new sterilization technique provided more contraceptive choices for these women, yet more contraceptive decision making autonomy, as well as more equitable social structures, are still needed. PMID:23761929

Effect of sterilization procedures on properties of plasma polymers relevant to biomedical applications

Energy Technology Data Exchange (ETDEWEB)

Artemenko, A. [Charles University in Prague, Faculty of Mathematics and Physics, Department of Macromolecular Physics, V Holesovickach 2, 180 00 Prague (Czech Republic); Kylian, O., E-mail: ondrej.kylian@gmail.com [Charles University in Prague, Faculty of Mathematics and Physics, Department of Macromolecular Physics, V Holesovickach 2, 180 00 Prague (Czech Republic); Choukourov, A.; Gordeev, I.; Petr, M. [Charles University in Prague, Faculty of Mathematics and Physics, Department of Macromolecular Physics, V Holesovickach 2, 180 00 Prague (Czech Republic); Vandrovcova, M. [Institute of Physiology, Academy of Sciences of the Czech Republic, Department of Growth and Differentiation of Cell Populations, Videnska 1083, 142 20, Prague 4 (Czech Republic); Polonskyi, O. [Charles University in Prague, Faculty of Mathematics and Physics, Department of Macromolecular Physics, V Holesovickach 2, 180 00 Prague (Czech Republic); Bacakova, L. [Institute of Physiology, Academy of Sciences of the Czech Republic, Department of Growth and Differentiation of Cell Populations, Videnska 1083, 142 20, Prague 4 (Czech Republic); Slavinska, D.; Biederman, H. [Charles University in Prague, Faculty of Mathematics and Physics, Department of Macromolecular Physics, V Holesovickach 2, 180 00 Prague (Czech Republic)

2012-10-01

This study is focused on the evaluation of resistance of plasma polymers toward common sterilization techniques, i.e. property important for possible use of such materials in biomedical applications. Three kinds of plasma polymers having different bioadhesive natures were studied: plasma polymerized poly(ethylene oxide), fluorocarbon plasma polymers, and nitrogen-rich plasma polymers. These plasma polymers were subjected to dry heat, autoclave and UV radiation treatment. Their physical, chemical and bioresponsive properties were determined by means of different techniques (ellipsometry, atomic force microscopy, wettability measurements, X-ray photoelectron spectroscopy and biological tests with osteoblast-like cells MG63). The results clearly show that properties of thin films of plasma polymers may be significantly altered by a sterilization process. Moreover, observed changes induced by selected sterilization methods were found to depend strongly on the sterilized plasma polymer. - Highlights: Black-Right-Pointing-Pointer Effect of common sterilization methods on three kinds of plasma polymers is studied. Black-Right-Pointing-Pointer Physical, chemical and bioresponsive properties of plasma polymers are analyzed. Black-Right-Pointing-Pointer Changes induced by sterilization depend strongly on type of the plasma polymer.

Heritable strategies for controlling insect vectors of disease.

Science.gov (United States)

Burt, Austin

2014-01-01

Mosquito-borne diseases are causing a substantial burden of mortality, morbidity and economic loss in many parts of the world, despite current control efforts, and new complementary approaches to controlling these diseases are needed. One promising class of new interventions under development involves the heritable modification of the mosquito by insertion of novel genes into the nucleus or of Wolbachia endosymbionts into the cytoplasm. Once released into a target population, these modifications can act to reduce one or more components of the mosquito population's vectorial capacity (e.g. the number of female mosquitoes, their longevity or their ability to support development and transmission of the pathogen). Some of the modifications under development are designed to be self-limiting, in that they will tend to disappear over time in the absence of recurrent releases (and hence are similar to the sterile insect technique, SIT), whereas other modifications are designed to be self-sustaining, spreading through populations even after releases stop (and hence are similar to traditional biological control). Several successful field trials have now been performed with Aedes mosquitoes, and such trials are helping to define the appropriate developmental pathway for this new class of intervention.

Remodeling of ACL Allografts is Inhibited by Peracetic Acid Sterilization

Science.gov (United States)

Gonnermann, Johannes; Kamp, Julia; Przybilla, Dorothea; Pruss, Axel

2008-01-01

Sterilization of allografts for anterior cruciate ligament (ACL) reconstruction has become an important prerequisite to prevent disease transmission. However, current sterilization techniques impair the biological or mechanical properties of such treated grafts. Peracetic acid (PAA) has been successfully used to sterilize bone allografts without these disadvantages and does not impair the mechanical properties of soft tissue grafts in vitro. We asked whether PAA sterilization would influence recellularization, restoration of crimp length and pattern, and revascularization of ACL grafts during early healing. We used an in vivo sheep model for open ACL reconstruction. We also correlated the histologic findings with the restoration of anteroposterior stability and structural properties during load-to-failure testing. PAA slowed remodeling activity at 6 and 12 weeks compared to nonsterilized allografts and autografts. The mechanical properties of PAA grafts were also reduced compared to these control groups at both time points. We conclude PAA sterilization currently should not be used to sterilize soft tissue grafts typically used in ACL reconstruction. PMID:18491201

Sterilization, high-level disinfection, and environmental cleaning.

Science.gov (United States)

Rutala, William A; Weber, David J

2011-03-01

Failure to perform proper disinfection and sterilization of medical devices may lead to introduction of pathogens, resulting in infection. New techniques have been developed for achieving high-level disinfection and adequate environmental cleanliness. This article examines new technologies for sterilization and high-level disinfection of critical and semicritical items, respectively, and because semicritical items carry the greatest risk of infection, the authors discuss reprocessing semicritical items such as endoscopes and automated endoscope reprocessors, endocavitary probes, prostate biopsy probes, tonometers, laryngoscopes, and infrared coagulation devices. In addition, current issues and practices associated with environmental cleaning are reviewed. Copyright © 2011. Published by Elsevier Inc.

Country status of application, manufacturing and sterilization of single-use medical products

International Nuclear Information System (INIS)

Norimah Yusof

1986-01-01

The paper reviews the current status of application of single-use medical products in Malaysia. The status of their manufacturing and sterilization is also discussed. The increasing production of such items calls for a more reliable and efficient sterilization technique in particular, radiation sterilization. In line with the demand and the effort to increase local production of medical products, UTN would be providing irradiation service together with research and development in this particular field by 1988. (author)

Evaluation of the effect of horse blood supplemented with human ...

African Journals Online (AJOL)

Tsetse flies are large biting insects that belong to the genus Glossina. Both .... and binary data (e.g mortality, pupae classes, etc) and b) one way analysis of variance for ..... Automation of tsetse mass rearing for use in sterile insect technique.

Radiation sterilization: an industrial process

International Nuclear Information System (INIS)

Ley, F.J.

1975-01-01

A new dimension has been added to the use of radiation in the medical field with the introduction of radiation as a sterilizing agent. Its use in diagnosis through radioactive tracers or X-rays and in therapy administered with the most sophisticated of electrical machines or radioisotope units, is familiar in the hospital world, being well established therein. In contrast, the application of radiation sterilization is in industry where the installation of large radiation sources is already commonplace in many countries. The beginnings in the early 1950's centered on the Van de Graaff machine and linear accelerators and the pioneering efforts of Ethicon Inc. here in the United States must be recognized. However, although sterilization with electron beams is still current practice in a number of plants, the use of gamma rays from cobalt-60 is preferred. The first steps in this direction were taken by the U.K.A.E.A. which, in common with similar organizations elsewhere, was attempting to exploit the tremendous potential for cobalt-60 production arising through the rapid construction of nuclear reactors. The first full-scale commercial gamma plant was commissioned in the U.K. in 1960. It reached a loading of 500,000 curies before its demolition after twelve years of operation. The process gained rapid acceptance within industry and approval by health authorities because it provided a ''cold'' sterilization method combining the property of lethal effect with penetration. Its immediate impact occurred in the introduction of disposable products making it possible, for example, to use heat-labile plastics and new packaging materials and package designs. Certainly, the technique has proved complementary to sterilization methods based on heat and to the use of chemical agents, in particular ethylene oxide gas

Radiation sterilization of livestock feeds

International Nuclear Information System (INIS)

Kawashima, Koji

1984-01-01

The radiation sterilization of livestock feeds is not much used presently because the process is not known well, and the cost is relatively high. However, its effect of sterilization is absolute, the radiation-sterilized feeds are safe in both nutrition and toxicity, and do not affect the appetite of livestocks, and the radiation energy required is small. In the future, as in the sterilization of medical supplies, feed radiation sterilization plants should be established, to stabilize livestock industry and to contribute to the health control of experimental animals. The following matters are described: radiation, comparison between radiation sterilization and other sterilization methods, the practice of feed radiation sterilization, the adverse effects of radiation sterilization, economic aspect, and the situation of feed radiation sterilization in various countries. (Mori, K.)

Electron beam sterilization of water discharged from sewage

International Nuclear Information System (INIS)

Miyata, Teijiro; Arai, Hidehiko; Tokunaga, Okihiro; Machi, Sueo; Kondo, Masaki; Minemura, Takashi; Nakao, Akio; Seike, Yasuhiko.

1989-01-01

At present, the water treated at city sewerages is discharged to rivers after the chlorine sterilization, but it was clarified recently that this chlorine treatment produces carcinogenic organic chlorine compounds, and residual chlorine exerts harmful effect to aquatics, therefore, it is desirable to develop the sterilization techniques substituting for chlorine treatment. Already many reports elucidated that irradiation is effective for the sterilization of the water discharged from sewerage. However, as the technical subject for putting radiation process in practical use, the treatment of large quantity was a problem. Recently by the progress of the technology of manufacturing electron accelerators, the equipment with large power output which can treat in large quantity was developed, and it has become applicable also to sewage treatment. Therefore, the authors examined the practicality of electron beam process as the substitute technology for chlorine sterilizaiton. In the case of using electron beam, though the power output of accelerators is large, the flight range of electron beam in water is short. The comparison of the sterilization effect of electron beam with that of Co-60 gamma ray, the effects of water depth, discharged water quality and water velocity on the sterilization effect and so on were experimentally examined. (K.I.)