Cytoplasmic Dynein Is Required for the Spatial Organization of Protein Aggregates in Filamentous Fungi

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Martin J. Egan

2015-04-01

Full Text Available Eukaryotes have evolved multiple strategies for maintaining cellular protein homeostasis. One such mechanism involves neutralization of deleterious protein aggregates via their defined spatial segregation. Here, using the molecular disaggregase Hsp104 as a marker for protein aggregation, we describe the spatial and temporal dynamics of protein aggregates in the filamentous fungus Aspergillus nidulans. Filamentous fungi, such as A. nidulans, are a diverse group of species of major health and economic importance and also serve as model systems for studying highly polarized eukaryotic cells. We find that microtubules promote the formation of Hsp104-positive aggregates, which coalesce into discrete subcellular structures in a process dependent on the microtubule-based motor cytoplasmic dynein. Finally, we find that impaired clearance of these inclusions negatively impacts retrograde trafficking of endosomes, a conventional dynein cargo, indicating that microtubule-based transport can be overwhelmed by chronic cellular stress.

Dynein's Network of Chemomechanical Motor Cycles

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Shen, Weibo; Wang, Ziqing; Wang, Guodong

2012-07-01

An eight-state network model of dynein's chemomechanical motor cycles is developed, in which the states of an effective single dynein head are represented by the number of ATP binding at the primary site and the number of ATP binding at other three secondary sites. The binding and unbinding of ATP, as well as the hydrolysis of ATP and the reverse process, are characterized by transition rates between certain states. Our results show that the stall force of dynein increases fast with ATP up to 1 mM ATP, beyond which it increases slowly to a saturated value, and that load and ATP concentration can adjust the step size of dynein, i.e., dynein can shift gears according to conditions. These results are in agreement with experiments [R. Mallik, B. C. Carter, S. A. Lex, S. J. King and S. P. Gross, Nature 427, 649 (2004)].

Clustering of Nuclei in Multinucleated Hyphae Is Prevented by Dynein-Driven Bidirectional Nuclear Movements and Microtubule Growth Control in Ashbya gossypii ▿ †

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Grava, Sandrine; Keller, Miyako; Voegeli, Sylvia; Seger, Shanon; Lang, Claudia; Philippsen, Peter

2011-01-01

During filamentous fungus development, multinucleated hyphae employ a system for long-range nuclear migration to maintain an equal nuclear density. A decade ago the microtubule motor dynein was shown to play a central role in this process. Previous studies with Ashbya gossypii revealed extensive bidirectional movements and bypassings of nuclei, an autonomous cytoplasmic microtubule (cMT) cytoskeleton emanating from each nucleus, and pulling of nuclei by sliding of cMTs along the cortex. Here, we show that dynein is the sole motor for bidirectional movements and bypassing because these movements are concomitantly decreased in mutants carrying truncations of the dynein heavy-chain DYN1 promoter. The dynactin component Jnm1, the accessory proteins Dyn2 and Ndl1, and the potential dynein cortical anchor Num1 are also involved in the dynamic distribution of nuclei. In their absence, nuclei aggregate to different degrees, whereby the mutants with dense nuclear clusters grow extremely long cMTs. As in budding yeast, we found that dynein is delivered to cMT plus ends, and its activity or processivity is probably controlled by dynactin and Num1. Together with its role in powering nuclear movements, we propose that dynein also plays (directly or indirectly) a role in the control of cMT length. Those combined dynein actions prevent nuclear clustering in A. gossypii and thus reveal a novel cellular role for dynein. PMID:21642510

Dynein Clusters into Lipid Microdomains on Phagosomes to Drive Rapid Transport toward Lysosomes.

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Rai, Ashim; Pathak, Divya; Thakur, Shreyasi; Singh, Shampa; Dubey, Alok Kumar; Mallik, Roop

2016-02-11

Diverse cellular processes are driven by motor proteins that are recruited to and generate force on lipid membranes. Surprisingly little is known about how membranes control the force from motors and how this may impact specific cellular functions. Here, we show that dynein motors physically cluster into microdomains on the membrane of a phagosome as it matures inside cells. Such geometrical reorganization allows many dyneins within a cluster to generate cooperative force on a single microtubule. This results in rapid directed transport of the phagosome toward microtubule minus ends, likely promoting phagolysosome fusion and pathogen degradation. We show that lipophosphoglycan, the major molecule implicated in immune evasion of Leishmania donovani, inhibits phagosome motion by disrupting the clustering and therefore the cooperative force generation of dynein. These findings appear relevant to several pathogens that prevent phagosome-lysosome fusion by targeting lipid microdomains on phagosomes. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Cytoplasmic Dynein Promotes HIV-1 Uncoating

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Paulina Pawlica

2014-11-01

Full Text Available Retroviral capsid (CA cores undergo uncoating during their retrograde transport (toward the nucleus, and/or after reaching the nuclear membrane. However, whether HIV-1 CA core uncoating is dependent upon its transport is not understood. There is some evidence that HIV-1 cores retrograde transport involves cytoplasmic dynein complexes translocating on microtubules. Here we investigate the role of dynein-dependent transport in HIV-1 uncoating. To interfere with dynein function, we depleted dynein heavy chain (DHC using RNA interference, and we over-expressed p50/dynamitin. In immunofluorescence microscopy experiments, DHC depletion caused an accumulation of CA foci in HIV-1 infected cells. Using a biochemical assay to monitor HIV-1 CA core disassembly in infected cells, we observed an increase in amounts of intact (pelletable CA cores upon DHC depletion or p50 over-expression. Results from these two complementary assays suggest that inhibiting dynein-mediated transport interferes with HIV-1 uncoating in infected cells, indicating the existence of a functional link between HIV-1 transport and uncoating.

Chlamydia trachomatis inclusion membrane protein CT850 interacts with the dynein light chain DYNLT1 (Tctex1).

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Mital, Jeffrey; Lutter, Erika I; Barger, Alexandra C; Dooley, Cheryl A; Hackstadt, Ted

2015-06-26

Chlamydia trachomatis actively subverts the minus-end directed microtubule motor, dynein, to traffic along microtubule tracks to the Microtubule Organizing Center (MTOC) where it remains within a membrane bound replicative vacuole for the duration of its intracellular development. Unlike most substrates of the dynein motor, disruption of the dynactin cargo-linking complex by over-expression of the p50 dynamitin subunit does not inhibit C. trachomatis transport. A requirement for chlamydial protein synthesis to initiate this process suggests that a chlamydial product supersedes a requirement for p50 dynamitin. A yeast 2-hybrid system was used to screen the chlamydia inclusion membrane protein CT850 against a HeLa cell cDNA library and identified an interaction with the dynein light chain DYNLT1 (Tctex1). This interaction was at least partially dependent upon an (R/K-R/K-X-X-R/K) motif that is characteristic of DYNLT1 binding domains. CT850 expressed ectopically in HeLa cells localized at the MTOC and this localization is similarly dependent upon the predicted DYNLT1 binding domain. Furthermore, DYNLT1 is enriched at focal concentrations of CT850 on the chlamydial inclusion membrane that are known to interact with dynein and microtubules. Depletion of DYNLT1 disrupts the characteristic association of the inclusion membrane with centrosomes. Collectively, the results suggest that CT850 interacts with DYNLT1 to promote appropriate positioning of the inclusion at the MTOC. Published by Elsevier Inc.

Dynein light chain family in Tetrahymena thermophila.

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Wilkes, David E; Rajagopalan, Vidyalakshmi; Chan, Clarence W C; Kniazeva, Ekaterina; Wiedeman, Alice E; Asai, David J

2007-02-01

Dyneins are large protein complexes that produce directed movement on microtubules. In situ, dyneins comprise combinations of heavy, intermediate, light-intermediate, and light chains. The light chains regulate the locations and activities of dyneins but their functions are not completely understood. We have searched the recently sequenced Tetrahymena thermophila macronuclear genome to describe the entire family of dynein light chains expressed in this organism. We identified fourteen genes encoding putative dynein light chains and seven genes encoding light chain-like proteins. RNA-directed PCR revealed that all 21 genes were expressed. Quantitative real time reverse transcription PCR showed that many of these genes were upregulated after deciliation, indicating that these proteins are present in cilia. Using the nomenclature developed in Chlamydomonas, Tetrahymena expresses two isoforms each of LC2, LC4, LC7, and Tctex1, three isoforms of p28, and six LC8/LC8-like isoforms. Tetrahymena also expresses two LC3-like genes. No Tetrahymena orthologue was found for Chlamydomonas LC5 or LC6. This study provides a complete description of the different genes and isoforms of the dynein light chains that are expressed in Tetrahymena, a model organism in which the targeted manipulation of genes is straightforward. (c) 2006 Wiley-Liss, Inc.

Catalytic Propulsion and Magnetic Steering of Soft, Patchy Microcapsules: Ability to Pick-Up and Drop-Off Microscale Cargo.

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Lu, Annie Xi; Liu, Yijing; Oh, Hyuntaek; Gargava, Ankit; Kendall, Eric; Nie, Zhihong; DeVoe, Don L; Raghavan, Srinivasa R

2016-06-22

We describe the creation of polymeric microcapsules that can exhibit autonomous motion along defined trajectories. The capsules are made by cross-linking aqueous microdroplets of the biopolymer chitosan using glutaraldehyde. A coflow microfluidic tubing device is used to generate chitosan droplets containing nanoparticles (NPs) with an iron (Fe) core and a platinum (Pt) shell. The droplets are then incubated in a Petri dish with the cross-linking solution, and an external magnet is placed below the Petri dish to pull the NPs together as a collective "patch" on one end of each droplet. This results in cross-linked capsules (∼150 μm in diameter) with an anisotropic (patchy) structure. When these capsules are placed in a solution of H2O2, the Pt shell of the NPs catalyzes the decomposition of H2O2 into O2 gas, which is ejected from the patchy end in the form of bubbles. As a result, the capsules (which are termed micromotors) move in a direction opposite to the bubbles. Furthermore, the micromotors can be steered along specific paths by an external magnet (the magnetic response arises due to the Fe in the core of the NPs). A given micromotor can thus be directed to meet with and adhere to an inert capsule, i.e., a model cargo. Adhesion occurs due to the soft nature of the two structures. Once the cargo is picked up, the micromotor-cargo pair can be moved along a specific path to a destination, whereupon the cargo can be released from the micromotor. We believe these soft micromotors offer significant benefits over their existing hard counterparts because of their biocompatibility, biodegradability, and ability to encapsulate a variety of payloads.

An investigation on motor-driven power steering-based crosswind disturbance compensation for the reduction of driver steering effort

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Kim, Kyuwon; Kim, Boemjun; Go, Youngil; Park, Jaeyong; Park, Joonhong; Suh, Insoo; Yi, Kyongsu

2014-07-01

This paper describes a lateral disturbance compensation algorithm for an application to a motor-driven power steering (MDPS)-based driver assistant system. The lateral disturbance including wind force and lateral load transfer by bank angle reduces the driver's steering refinement and at the same time increases the possibility of an accident. A lateral disturbance compensation algorithm is designed to determine the motor overlay torque of an MDPS system for reducing the manoeuvreing effort of a human driver under lateral disturbance. Motor overlay torque for the compensation of driver's steering torque induced by the lateral disturbance consists of human torque feedback and feedforward torque. Vehicle-driver system dynamics have been investigated using a combined dynamic model which consists of a vehicle dynamic model, driver steering dynamic model and lateral disturbance model. The human torque feedback input has been designed via the investigation of the vehicle-driver system dynamics. Feedforward input torque is calculated to compensate additional tyre self-aligning torque from an estimated lateral disturbance. The proposed compensation algorithm has been implemented on a developed driver model which represents the driver's manoeuvreing characteristics under the lateral disturbance. The developed driver model has been validated with test data via a driving simulator in a crosswind condition. Human-in-the-loop simulations with a full-scale driving simulator on a virtual test track have been conducted to investigate the real-time performance of the proposed lateral disturbance compensation algorithm. It has been shown from simulation studies and human-in-the-loop simulation results that the driver's manoeuvreing effort and a lateral deviation of the vehicle under the lateral disturbance can be significantly reduced via the lateral disturbance compensation algorithm.

Microemulsion-Based Soft Bacteria-Driven Microswimmers for Active Cargo Delivery.

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Singh, Ajay Vikram; Hosseinidoust, Zeinab; Park, Byung-Wook; Yasa, Oncay; Sitti, Metin

2017-10-24

Biohybrid cell-driven microsystems offer unparalleled possibilities for realization of soft microrobots at the micron scale. Here, we introduce a bacteria-driven microswimmer that combines the active locomotion and sensing capabilities of bacteria with the desirable encapsulation and viscoelastic properties of a soft double-micelle microemulsion for active transport and delivery of cargo (e.g., imaging agents, genes, and drugs) to living cells. Quasi-monodisperse double emulsions were synthesized with an aqueous core that encapsulated the fluorescence imaging agents, as a proof-of-concept cargo in this study, and an outer oil shell that was functionalized with streptavidin for specific and stable attachment of biotin-conjugated Escherichia coli. Motile bacteria effectively propelled the soft microswimmers across a Transwell membrane, actively delivering imaging agents (i.e., dyes) encapsulated inside of the micelles to a monolayer of cultured MCF7 breast cancer and J744A.1 macrophage cells, which enabled real-time, live-cell imaging of cell organelles, namely mitochondria, endoplasmic reticulum, and Golgi body. This in vitro model demonstrates the proof-of-concept feasibility of the proposed soft microswimmers and offers promise for potential biomedical applications in active and/or targeted transport and delivery of imaging agents, drugs, stem cells, siRNA, and therapeutic genes to live tissue in in vitro disease models (e.g., organ-on-a-chip devices) and stagnant or low-flow-velocity fluidic regions of the human body.

The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear.

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Colantonio, Jessica R; Vermot, Julien; Wu, David; Langenbacher, Adam D; Fraser, Scott; Chen, Jau-Nian; Hill, Kent L

2009-01-08

In teleosts, proper balance and hearing depend on mechanical sensors in the inner ear. These sensors include actin-based microvilli and microtubule-based cilia that extend from the surface of sensory hair cells and attach to biomineralized 'ear stones' (or otoliths). Otolith number, size and placement are under strict developmental control, but the mechanisms that ensure otolith assembly atop specific cells of the sensory epithelium are unclear. Here we demonstrate that cilia motility is required for normal otolith assembly and localization. Using in vivo video microscopy, we show that motile tether cilia at opposite poles of the otic vesicle create fluid vortices that attract otolith precursor particles, thereby biasing an otherwise random distribution to direct localized otolith seeding on tether cilia. Independent knockdown of subunits for the dynein regulatory complex and outer-arm dynein disrupt cilia motility, leading to defective otolith biogenesis. These results demonstrate a requirement for the dynein regulatory complex in vertebrates and show that cilia-driven flow is a key epigenetic factor in controlling otolith biomineralization.

Cytoplasmic Dynein Regulation by Subunit Heterogeneity and Its Role in Apical Transport

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Tai, Andrew W.; Chuang, Jen-Zen; Sung, Ching-Hwa

2001-01-01

Despite the existence of multiple subunit isoforms for the microtubule motor cytoplasmic dynein, it has not yet been directly shown that dynein complexes with different compositions exhibit different properties. The 14-kD dynein light chain Tctex-1, but not its homologue RP3, binds directly to rhodopsin's cytoplasmic COOH-terminal tail, which encodes an apical targeting determinant in polarized epithelial Madin-Darby canine kidney (MDCK) cells. We demonstrate that Tctex-1 and RP3 compete for binding to dynein intermediate chain and that overexpressed RP3 displaces endogenous Tctex-1 from dynein complexes in MDCK cells. Furthermore, replacement of Tctex-1 by RP3 selectively disrupts the translocation of rhodopsin to the MDCK apical surface. These results directly show that cytoplasmic dynein function can be regulated by its subunit composition and that cytoplasmic dynein is essential for at least one mode of apical transport in polarized epithelia. PMID:11425878

Huntingtin coordinates the dynein-mediated dynamic positioning of endosomes and lysosomes

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Caviston, Juliane P.; Zajac, Allison L.; Tokito, Mariko; Holzbaur, Erika L.F.

2011-01-01

Huntingtin (Htt) is a membrane-associated scaffolding protein that interacts with microtubule motors as well as actin-associated adaptor molecules. We examined a role for Htt in the dynein-mediated intracellular trafficking of endosomes and lysosomes. In HeLa cells depleted of either Htt or dynein, early, recycling, and late endosomes (LE)/lysosomes all become dispersed. Despite altered organelle localization, kinetic assays indicate only minor defects in intracellular trafficking. Expression of full-length Htt is required to restore organelle localization in Htt-depleted cells, supporting a role for Htt as a scaffold that promotes functional interactions along its length. In dynein-depleted cells, LE/lysosomes accumulate in tight patches near the cortex, apparently enmeshed by cortactin-positive actin filaments; Latrunculin B-treatment disperses these patches. Peripheral LE/lysosomes in dynein-depleted cells no longer colocalize with microtubules. Htt may be required for this off-loading, as the loss of microtubule association is not seen in Htt-depleted cells or in cells depleted of both dynein and Htt. Inhibition of kinesin-1 relocalizes peripheral LE/lysosomes induced by Htt depletion but not by dynein depletion, consistent with their detachment from microtubules upon dynein knockdown. Together, these data support a model of Htt as a facilitator of dynein-mediated trafficking that may regulate the cytoskeletal association of dynamic organelles. PMID:21169558

46 CFR 32.50-35 - Remote manual shutdown for internal combustion engine driven cargo pump on tank vessels-TB/ALL.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Remote manual shutdown for internal combustion engine... for Cargo Handling § 32.50-35 Remote manual shutdown for internal combustion engine driven cargo pump on tank vessels—TB/ALL. (a) Any tank vessel which is equipped with an internal combustion engine...

High-performance beam steering using electrowetting-driven liquid prism fabricated by a simple dip-coating method

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Enrico Clement, Carlos; Park, Sung-Yong, E-mail: mpeps@nus.edu.sg [Department of Mechanical Engineering, National University of Singapore, Block EA, #07-08, 9 Engineering Drive 1, 117576 (Singapore)

2016-05-09

A high degree of beam steering is demonstrated using an electrowetting-driven liquid prism. While prism devices have typically relied on complex and expensive laboratory setups, such as high-vacuum facilities for fabrication of dielectric layers, this work utilizes a simple dip-coating method to provide an ion gel layer as a dielectric, offering 2 or 3 orders higher specific capacitance (c ≈ 10 μF/cm{sup 2}) than that of conventional dielectrics. Analytical studies present the effects of liquid selection and arrangement on overall prism performance. For experimental demonstrations of high-performance beam steering, we not only selected two immiscible liquids of water and 1-bromonaphthalene (1-BN) oil which provide the large refractive index difference (n{sub water} = 1.33 and n{sub 1-BN} = 1.65 at λ = 532 nm) between them, but also utilized a double-stacked prism configuration which increases the number of interfaces for incoming light to be steered. At a prism apex angle of φ = 27°, we were able to achieve significantly large beam steering of up to β = 19.06°, which is the highest beam steering performance ever demonstrated using electrowetting technology.

Dync1h1 Mutation Causes Proprioceptive Sensory Neuron Loss and Impaired Retrograde Axonal Transport of Dorsal Root Ganglion Neurons.

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Zhao, Jing; Wang, Yi; Xu, Huan; Fu, Yuan; Qian, Ting; Bo, Deng; Lu, Yan-Xin; Xiong, Yi; Wan, Jun; Zhang, Xiang; Dong, Qiang; Chen, Xiang-Jun

2016-07-01

Sprawling (Swl) is a radiation-induced mutation which has been identified to have a nine base pair deletion in dynein heavy chain 1 (DYNC1H1: encoded by a single gene Dync1h1). This study is to investigate the phenotype and the underlying mechanism of the Dync1h1 mutant. To display the phenotype of Swl mutant mice, we examined the embryos of homozygous (Swl/Swl) and heterozygous (Swl/+) mice and their postnatal dorsal root ganglion (DRG) of surviving Swl/+ mice. The Swl/+ mice could survive for a normal life span, while Swl/Swl could only survive till embryonic (E) 8.5 days. Excessive apoptosis of Swl/+ DRG neurons was revealed during E11.5-E15.5 days, and the peak rate was at E13.5 days. In vitro study of mutated DRG neurons showed impaired retrograde transport of dynein-driven nerve growth factor (NGF). Mitochondria, another dynein-driven cargo, demonstrated much slower retrograde transport velocity in Swl/+ neurons than in wild-type (WT) neurons. Nevertheless, the Swl, Loa, and Cra mutations did not affect homodimerization of DYNC1H1. The Swl/Swl mutation of Dync1h1 gene led to embryonic mal-development and lethality, whereas the Swl/+ DRG neurons demonstrated deficient retrograde transport in dynein-driven cargos and excessive apoptosis during mid- to late-developmental stages. The underlying mechanism of the mutation may not be due to impaired homodimerization of DYNC1H1. © 2016 John Wiley & Sons Ltd.

Time-Dependent Measure of a Nano-Scale Force-Pulse Driven by the Axonemal Dynein Motors in Individual Live Sperm Cells

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Allen, M J; Rudd, R E; McElfresh, M W; Balhorn, R

2009-04-23

Nano-scale mechanical forces generated by motor proteins are crucial to normal cellular and organismal functioning. The ability to measure and exploit such forces would be important to developing motile biomimetic nanodevices powered by biological motors for Nanomedicine. Axonemal dynein motors positioned inside the sperm flagellum drive microtubule sliding giving rise to rhythmic beating of the flagellum. This force-generating action makes it possible for the sperm cell to move through viscous media. Here we report new nano-scale information on how the propulsive force is generated by the sperm flagellum and how this force varies over time. Single cell recordings reveal discrete {approx}50 ms pulses oscillating with amplitude 9.8 {+-} 2.6 nN independent of pulse frequency (3.5-19.5 Hz). The average work carried out by each cell is 4.6 x 10{sup -16} J per pulse, equivalent to the hydrolysis of {approx}5,500 ATP molecules. The mechanochemical coupling at each active dynein head is {approx}2.2 pN/ATP, and {approx}3.9 pN per dynein arm, in agreement with previously published values obtained using different methods.

Live Cell Imaging Reveals Differential Modifications to Cytoplasmic Dynein Properties by Phospho- and Dephospho-mimic Mutations of the Intermediate Chain 2C S84

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Blasier, Kiev R.; Humsi, Michael K.; Ha, Junghoon; Ross, Mitchell W.; Smiley, W. Russell; Inamdar, Nirja A.; Mitchell, David J.; Lo, Kevin W.-H.; Pfister, K. Kevin

2014-01-01

Cytoplasmic dynein is a multi-subunit motor protein responsible for intracellular cargo transport toward microtubule minus ends. There are multiple isoforms of the dynein intermediate chain (DYNC1I, IC) which is encoded by two genes. One way to regulate cytoplasmic dynein is by IC phosphorylation. The IC-2C isoform is expressed in all cells and the functional significance of phosphorylation on IC-2C serine 84 was investigated using live cell imaging of fluorescent protein-tagged wild type IC-2C (WT) and phospho- and dephospho-mimic mutant isoforms in axonal transport model systems. Both mutations modulated dynein functional properties. The dephospho-mimic mutant IC-2C S84A had greater co-localization with mitochondria than IC-2C wild-type (WT) or the phospho-mimic mutant IC-2C S84D. The dephospho-mimic mutant IC-2C S84A was also more likely to be motile than the phospho-mimic mutant IC-2C S84D or IC-2C WT. In contrast, the phospho-mimic mutant IC-2C S84D mutant was more likely to move in the retrograde direction than was the IC-2C S84A mutant. The phospho-mimic IC-2C S84D was also as likely as IC-2C WT to co-localize with mitochondria. Both the S84D phospho- and S84A, dephospho-mimic mutants were found to be capable of microtubule minus end directed (retrograde) movement in axons. They were also observed to be passively transported in the anterograde direction. These data suggest that the IC-2C S84 has a role in modulating dynein properties. PMID:24798412

Dyneins: structure, biology and disease

National Research Council Canada - National Science Library

King, Stephen M

2012-01-01

.... From bench to bedside, Dynein: Structure, Biology and Disease offers research on fundamental cellular processes to researchers and clinicians across developmental biology, cell biology, molecular biology, biophysics, biomedicine...

Genetic analysis of the cytoplasmic dynein subunit families.

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Pfister, K Kevin; Shah, Paresh R; Hummerich, Holger; Russ, Andreas; Cotton, James; Annuar, Azlina Ahmad; King, Stephen M; Fisher, Elizabeth M C

2006-01-01

Cytoplasmic dyneins, the principal microtubule minus-end-directed motor proteins of the cell, are involved in many essential cellular processes. The major form of this enzyme is a complex of at least six protein subunits, and in mammals all but one of the subunits are encoded by at least two genes. Here we review current knowledge concerning the subunits, their interactions, and their functional roles as derived from biochemical and genetic analyses. We also carried out extensive database searches to look for new genes and to clarify anomalies in the databases. Our analysis documents evolutionary relationships among the dynein subunits of mammals and other model organisms, and sheds new light on the role of this diverse group of proteins, highlighting the existence of two cytoplasmic dynein complexes with distinct cellular roles.

Genetic analysis of the cytoplasmic dynein subunit families.

Directory of Open Access Journals (Sweden)

K Kevin Pfister

2006-01-01

Full Text Available Cytoplasmic dyneins, the principal microtubule minus-end-directed motor proteins of the cell, are involved in many essential cellular processes. The major form of this enzyme is a complex of at least six protein subunits, and in mammals all but one of the subunits are encoded by at least two genes. Here we review current knowledge concerning the subunits, their interactions, and their functional roles as derived from biochemical and genetic analyses. We also carried out extensive database searches to look for new genes and to clarify anomalies in the databases. Our analysis documents evolutionary relationships among the dynein subunits of mammals and other model organisms, and sheds new light on the role of this diverse group of proteins, highlighting the existence of two cytoplasmic dynein complexes with distinct cellular roles.

Dynein Transmits Polarized Actomyosin Cortical Flows to Promote Centrosome Separation

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Alessandro De Simone

2016-03-01

Full Text Available The two centrosomes present at the onset of mitosis must separate in a timely and accurate fashion to ensure proper bipolar spindle assembly. The minus-end-directed motor dynein plays a pivotal role in centrosome separation, but the underlying mechanisms remain elusive, particularly regarding how dynein coordinates this process in space and time. We addressed these questions in the one-cell C. elegans embryo, using a combination of 3D time-lapse microscopy and computational modeling. Our analysis reveals that centrosome separation is powered by the joint action of dynein at the nuclear envelope and at the cell cortex. Strikingly, we demonstrate that dynein at the cell cortex acts as a force-transmitting device that harnesses polarized actomyosin cortical flows initiated by the centrosomes earlier in the cell cycle. This mechanism elegantly couples cell polarization with centrosome separation, thus ensuring faithful cell division.

How molecular motors are arranged on a cargo is important for vesicular transport.

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Robert P Erickson

2011-05-01

Full Text Available The spatial organization of the cell depends upon intracellular trafficking of cargos hauled along microtubules and actin filaments by the molecular motor proteins kinesin, dynein, and myosin. Although much is known about how single motors function, there is significant evidence that cargos in vivo are carried by multiple motors. While some aspects of multiple motor function have received attention, how the cargo itself--and motor organization on the cargo--affects transport has not been considered. To address this, we have developed a three-dimensional Monte Carlo simulation of motors transporting a spherical cargo, subject to thermal fluctuations that produce both rotational and translational diffusion. We found that these fluctuations could exert a load on the motor(s, significantly decreasing the mean travel distance and velocity of large cargos, especially at large viscosities. In addition, the presence of the cargo could dramatically help the motor to bind productively to the microtubule: the relatively slow translational and rotational diffusion of moderately sized cargos gave the motors ample opportunity to bind to a microtubule before the motor/cargo ensemble diffuses out of range of that microtubule. For rapidly diffusing cargos, the probability of their binding to a microtubule was high if there were nearby microtubules that they could easily reach by translational diffusion. Our simulations found that one reason why motors may be approximately 100 nm long is to improve their 'on' rates when attached to comparably sized cargos. Finally, our results suggested that to efficiently regulate the number of active motors, motors should be clustered together rather than spread randomly over the surface of the cargo. While our simulation uses the specific parameters for kinesin, these effects result from generic properties of the motors, cargos, and filaments, so they should apply to other motors as well.

Emergence of flagellar beating from the collective behavior of individual ATP-powered dyneins

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Namdeo, S.; Onck, P. R.

2016-10-01

Flagella are hair-like projections from the surface of eukaryotic cells, and they play an important role in many cellular functions, such as cell-motility. The beating of flagella is enabled by their internal architecture, the axoneme, and is powered by a dense distribution of motor proteins, dyneins. The dyneins deliver the required mechanical work through the hydrolysis of ATP. Although the dynein-ATP cycle, the axoneme microstructure, and the flagellar-beating kinematics are well studied, their integration into a coherent picture of ATP-powered flagellar beating is still lacking. Here we show that a time-delayed negative-work-based switching mechanism is able to convert the individual sliding action of hundreds of dyneins into a regular overall beating pattern leading to propulsion. We developed a computational model based on a minimal representation of the axoneme consisting of two representative doublet microtubules connected by nexin links. The relative sliding of the microtubules is incorporated by modeling two groups of ATP-powered dyneins, each responsible for sliding in opposite directions. A time-delayed switching mechanism is postulated, which is key in converting the local individual sliding action of multiple dyneins into global beating. Our results demonstrate that an overall nonreciprocal beating pattern can emerge with time due to the spatial and temporal coordination of the individual dyneins. These findings provide insights in the fundamental working mechanism of axonemal dyneins and could possibly open new research directions in the field of flagellar motility.

Calcium regulates ATP-sensitive microtubule binding by Chlamydomonas outer arm dynein.

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Sakato, Miho; King, Stephen M

2003-10-31

The Chlamydomonas outer dynein arm contains three distinct heavy chains (alpha, beta, and gamma) that exhibit different motor properties. The LC4 protein, which binds 1-2 Ca2+ with KCa = 3 x 10-5 m, is associated with the gamma heavy chain and has been proposed to act as a sensor to regulate dynein motor function in response to alterations in intraflagellar Ca2+ levels. Here we genetically dissect the outer arm to yield subparticles containing different motor unit combinations and assess the microtubule-binding properties of these complexes both prior to and following preincubation with tubulin and ATP, which was used to inhibit ATP-insensitive (structural) microtubule binding. We observed that the alpha heavy chain exhibits a dominant Ca2+-independent ATP-sensitive MT binding activity in vitro that is inhibited by attachment of tubulin to the structural microtubule-binding domain. Furthermore, we show that ATP-sensitive microtubule binding by a dynein subparticle containing only the beta and gamma heavy chains does not occur at Ca2+ concentrations below pCa 6 but is maximally activated above pCa 5. This activity was not observed in mutant dyneins containing small deletions in the microtubule-binding region of the beta heavy chain or in dyneins that lack both the alpha heavy chain and the motor domain of the beta heavy chain. These findings strongly suggest that Ca2+ binding directly to a component of the dynein complex regulates ATP-sensitive interactions between the beta heavy chain and microtubules and lead to a model for how individual motor units are controlled within the outer dynein arm.

Diverse Roles of Axonemal Dyneins in Drosophila Auditory Neuron Function and Mechanical Amplification in Hearing.

Science.gov (United States)

Karak, Somdatta; Jacobs, Julie S; Kittelmann, Maike; Spalthoff, Christian; Katana, Radoslaw; Sivan-Loukianova, Elena; Schon, Michael A; Kernan, Maurice J; Eberl, Daniel F; Göpfert, Martin C

2015-11-26

Much like vertebrate hair cells, the chordotonal sensory neurons that mediate hearing in Drosophila are motile and amplify the mechanical input of the ear. Because the neurons bear mechanosensory primary cilia whose microtubule axonemes display dynein arms, we hypothesized that their motility is powered by dyneins. Here, we describe two axonemal dynein proteins that are required for Drosophila auditory neuron function, localize to their primary cilia, and differently contribute to mechanical amplification in hearing. Promoter fusions revealed that the two axonemal dynein genes Dmdnah3 (=CG17150) and Dmdnai2 (=CG6053) are expressed in chordotonal neurons, including the auditory ones in the fly's ear. Null alleles of both dyneins equally abolished electrical auditory neuron responses, yet whereas mutations in Dmdnah3 facilitated mechanical amplification, amplification was abolished by mutations in Dmdnai2. Epistasis analysis revealed that Dmdnah3 acts downstream of Nan-Iav channels in controlling the amplificatory gain. Dmdnai2, in addition to being required for amplification, was essential for outer dynein arms in auditory neuron cilia. This establishes diverse roles of axonemal dyneins in Drosophila auditory neuron function and links auditory neuron motility to primary cilia and axonemal dyneins. Mutant defects in sperm competition suggest that both dyneins also function in sperm motility.

How Insect Flight Steering Muscles Work

OpenAIRE

Walker, Simon M.; Schwyn, Daniel A.; Mokso, Rajmund; Wicklein, Martina; Müller, Tonya; Doube, Michael; Stampanoni, Marco; Krapp, Holger G.; Taylor, Graham K.

2014-01-01

Dipteran flies are amongst the smallest and most agile of flying animals. Their wings are driven indirectly by large power muscles, which cause cyclical deformations of the thorax that are amplified through the intricate wing hinge. Asymmetric flight manoeuvres are controlled by 13 pairs of steering muscles acting directly on the wing articulations. Collectively the steering muscles account for

Dynein is the motor for retrograde axonal transport of organelles

International Nuclear Information System (INIS)

Schnapp, B.J.; Reese, T.S.

1989-01-01

Vesicular organelles in axons of nerve cells are transported along microtubules either toward their plus ends (fast anterograde transport) or toward their minus ends (retrograde transport). Two microtubule-based motors were previously identified by examining plastic beads induced to move along microtubules by cytosol fractions from the squid giant axon: (i) an anterograde motor, kinesin, and (ii) a retrograde motor, which is characterized here. The retrograde motor, a cytosolic protein previously termed HMW1, was purified from optic lobes and extruded axoplasm by nucleotide-dependent microtubule affinity and release; microtubule gliding was used as the assay of motor activity. The following properties of the retrograde motor suggest that it is cytoplasmic dynein: (i) sedimentation at 20-22 S with a heavy chain of Mr greater than 200,000 that coelectrophoreses with the alpha and beta subunits of axonemal dynein, (ii) cleavage by UV irradiation in the presence of ATP and vanadate, and (iii) a molecular structure resembling two-headed dynein from axonemes. Furthermore, bead movement toward the minus end of microtubules was blocked when axoplasmic supernatants were treated with UV/vanadate. Treatment of axoplasmic supernatant with UV/vanadate also blocks the retrograde movement of purified organelles in vitro without changing the number of anterograde moving organelles, indicating that dynein interacts specifically with a subgroup of organelles programmed to move toward the cell body. However, purified optic lobe dynein, like purified kinesin, does not by itself promote the movement of purified organelles along microtubules, suggesting that additional axoplasmic factors are necessary for retrograde as well as anterograde transport

Emergence of flagellar beating from the collective behavior of individual ATP-powered dyneins

OpenAIRE

Namdeo, S.; Onck, P. R.

2016-01-01

Flagella are hair-like projections from the surface of eukaryotic cells, and they play an important role in many cellular functions, such as cell-motility. The beating of flagella is enabled by their internal architecture, the axoneme, and is powered by a dense distribution of motor proteins, dyneins. The dyneins deliver the required mechanical work through the hydrolysis of ATP. Although the dynein-ATP cycle, the axoneme microstructure, and the flagellar-beating kinematics are well studied, ...

Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance

Institute of Scientific and Technical Information of China (English)

Yihan Wan; Zhenye Yang; Jing Guo; Qiangge Zhang; Liyong Zeng; Wei Song; Yue Xiao; Xueliang Zhu

2012-01-01

The Gβγ heterodimer is an important signal transducer.Gβ,however,is prone to misfolding due to its requirement for Gγ and chaperones for proper folding.How cells dispose of misfolded Gβ (mfGβ) is not clear.Here,we showed that mfGβ was able to be polyubiquitinated and subsequently degraded by the proteasome.It was sequestered in aggresomes after the inhibition of the proteasome activity with MG132.Sustained activation of Gβγ signaling further elevated cellular levels of the ubiquitinated Gβ.Moreover,Nudel,a regulator of cytoplasmic dynein,the microtubule minus end-directed motor,directly interacted with both the unubiquitinated and ubiquitinated mfGβ.Increasing the levels of both mfGβ and Nudel promoted the association of Gβ with both Nudel and dynein,resulting in robust aggresome formation in a dynein-dependent manner.Depletion of Nudel by RNAi reduced the dynein-associated mfGβ,impaired the MG132-induced aggresome formation,and markedly prolonged the half-life of nascent Gβ.Therefore,cytosolic mfGβ is recruited to dynein by Nudel and transported to the centrosome for rapid sequestration and degradation.Such a process not only eliminates mfGβ efficiently for the control of protein quality,but may also help to terminate the Gβγ signaling.

Fundamental X-mode electron cyclotron current drive using remote-steering symmetric direction antenna at larger steering angles

International Nuclear Information System (INIS)

Idei, H.; Hanada, K.; Zushi, H.; Sato, K.N.; Sakamoto, M.; Iyomasa, A.; Kawasaki, S.; Nakashima, H.; Higashijima, A.; Ohkubo, K.; Kubo, S.; Shimozuma, T.; Ito, S.; Hasegawa, M.; Nakamura, K.; Notake, T.; Hoshika, H.; Maezono, N.; Nishi, S.; Nakashima, K.

2005-01-01

A remote steering antenna has been newly developed for Electron Cyclotron Heating and Current Drive (ECH/ECCD) experiments on the TRIAM-1M tokamak. It is a first application of the remote steering antenna to the ECH/ECCD experiments under the conditions relevant to International Thermonuclear Experimental Reactor. Our launcher is a symmetric direction antenna with extended steering capability. The larger steering angles of 8-19 degrees are available, in addition to that near 0 degree. The output beam from the antenna is the well-defined Gaussian beam with a correct steering angle. The Gaussian content and the steering angle accuracy are 0.85 and -0.3 degrees, respectively. Antenna transmission efficiency in the high power test is evaluated as 0.95. The efficiencies at the low and high power tests are consistent with those in the calculation with higher-order modes. The difference between plasma currents increased at co- and counter-steering injections [+/-19 degrees] is clearly observed in the superposition to the Lower Hybrid Current Driven (LHCD) plasma of the fundamental X-mode injection. (author)

Functional Architecture of the Outer Arm Dynein Conformational Switch*

Science.gov (United States)

King, Stephen M.; Patel-King, Ramila S.

2012-01-01

Dynein light chain 1 (LC1/DNAL1) is one of the most highly conserved components of ciliary axonemal outer arm dyneins, and it associates with both a heavy chain motor unit and tubulin located within the A-tubule of the axonemal outer doublet microtubules. In a variety of model systems, lack of LC1 or expression of mutant forms leads to profound defects in ciliary motility, including the failure of the hydrodynamic coupling needed for ciliary metachronal synchrony, random stalling during the power/recovery stroke transition, an aberrant response to imposed viscous load, and in some cases partial failure of motor assembly. These phenotypes have led to the proposal that LC1 acts as part of a mechanical switch to control motor function in response to alterations in axonemal curvature. Here we have used NMR chemical shift mapping to define the regions perturbed by a series of mutations in the C-terminal domain that yield a range of phenotypic effects on motility. In addition, we have identified the subdomain of LC1 involved in binding microtubules and characterized the consequences of an Asn → Ser alteration within the terminal leucine-rich repeat that in humans causes primary ciliary dyskinesia. Together, these data define a series of functional subdomains within LC1 and allow us to propose a structural model for the organization of the dynein heavy chain-LC1-microtubule ternary complex that is required for the coordinated activity of dynein motors in cilia. PMID:22157010

CCDC151 Mutations Cause Primary Ciliary Dyskinesia by Disruption of the Outer Dynein Arm Docking Complex Formation

NARCIS (Netherlands)

Hjeij, R.; Onoufriadis, A.; Watson, C.M.; Slagle, C.E.; Klena, N.T.; Dougherty, G.W.; Kurkowiak, M.; Loges, N.T.; Diggle, C.P.; Morante, N.F.; Gabriel, G.C.; Lemke, K.L.; Li, Y.; Pennekamp, P.; Menchen, T.; Konert, F.; Marthin, J.K.; Mans, D.A.; Letteboer, S.J.F.; Werner, C.; Burgoyne, T.; Westermann, C.; Rutman, A.; Carr, I.M.; O'Callaghan, C.; Moya, E.; Chung, E.M.; Consortium, U.K.; Sheridan, E.; Nielsen, K.G.; Roepman, R.; Bartscherer, K.; Burdine, R.D.; Lo, C.W.; Omran, H.; Mitchison, H.M.

2014-01-01

A diverse family of cytoskeletal dynein motors powers various cellular transport systems, including axonemal dyneins generating the force for ciliary and flagellar beating essential to movement of extracellular fluids and of cells through fluid. Multisubunit outer dynein arm (ODA) motor complexes,

Calcium sensors of ciliary outer arm dynein: functions and phylogenetic considerations for eukaryotic evolution.

Science.gov (United States)

Inaba, Kazuo

2015-01-01

The motility of eukaryotic cilia and flagella is modulated in response to several extracellular stimuli. Ca(2+) is the most critical intracellular factor for these changes in motility, directly acting on the axonemes and altering flagellar asymmetry. Calaxin is an opisthokont-specific neuronal calcium sensor protein first described in the sperm of the ascidian Ciona intestinalis. It binds to a heavy chain of two-headed outer arm dynein in a Ca(2+)-dependent manner and regulates 'asymmetric' wave propagation at high concentrations of Ca(2+). A Ca(2+)-binding subunit of outer arm dynein in Chlamydomonas reinhardtii, the light chain 4 (LC4), which is a Ca(2+)-sensor phylogenetically different from calaxin, shows Ca(2+)-dependent binding to a heavy chain of three-headed outer arm dynein. However, LC4 appears to participate in 'symmetric' wave propagation at high concentrations of Ca(2+). LC4-type dynein light chain is present in bikonts, except for some subclasses of the Excavata. Thus, flagellar asymmetry-symmetry conversion in response to Ca(2+) concentration represents a 'mirror image' relationship between Ciona and Chlamydomonas. Phylogenetic analyses indicate the duplication, divergence, and loss of heavy chain and Ca(2+)-sensors of outer arm dynein among excavate species. These features imply a divergence point with respect to Ca(2+)-dependent regulation of outer arm dynein in cilia and flagella during the evolution of eukaryotic supergroups.

LRRC6 mutation causes primary ciliary dyskinesia with dynein arm defects.

Directory of Open Access Journals (Sweden)

Amjad Horani

Full Text Available Despite recent progress in defining the ciliome, the genetic basis for many cases of primary ciliary dyskinesia (PCD remains elusive. We evaluated five children from two unrelated, consanguineous Palestinian families who had PCD with typical clinical features, reduced nasal nitric oxide concentrations, and absent dynein arms. Linkage analyses revealed a single common homozygous region on chromosome 8 and one candidate was conserved in organisms with motile cilia. Sequencing revealed a single novel mutation in LRRC6 (Leucine-rich repeat containing protein 6 that fit the model of autosomal recessive genetic transmission, leading to a change of a highly conserved amino acid from aspartic acid to histidine (Asp146His. LRRC6 was localized to the cytoplasm and was up-regulated during ciliogenesis in human airway epithelial cells in a Foxj1-dependent fashion. Nasal epithelial cells isolated from affected individuals and shRNA-mediated silencing in human airway epithelial cells, showed reduced LRRC6 expression, absent dynein arms, and slowed cilia beat frequency. Dynein arm proteins were either absent or mislocalized to the cytoplasm in airway epithelial cells from a primary ciliary dyskinesia subject. These findings suggest that LRRC6 plays a role in dynein arm assembly or trafficking and when mutated leads to primary ciliary dyskinesia with laterality defects.

Operational experience with model-based steering in the SLC linac

International Nuclear Information System (INIS)

Thompson, K.A.; Himel, T.; Moore, S.; Sanchez-Chopitea, L.; Shoaee, H.

1989-03-01

Operational experience with model-driven steering in the linac of the Stanford Linear Collider is discussed. Important issues include two-beam steering, sensitivity of algorithms to faulty components, sources of disagreement with the model, and the effects of the finite resolution of beam position monitors. Methods developed to make the steering algorithms more robust in the presence of such complications are also presented. 5 refs., 1 fig

Cdk1 Activates Pre-Mitotic Nuclear Envelope Dynein Recruitment and Apical Nuclear Migration in Neural Stem cells

Science.gov (United States)

Baffet, Alexandre D.; Hu, Daniel J.; Vallee, Richard B.

2015-01-01

Summary Dynein recruitment to the nuclear envelope is required for pre-mitotic nucleus-centrosome interactions in nonneuronal cells, and for apical nuclear migration in neural stem cells. In each case, dynein is recruited to the nuclear envelope (NE) specifically during G2, via two nuclear pore-mediated mechanisms involving RanBP2-BicD2 and Nup133-CENP-F. The mechanisms responsible for cell cycle control of this behavior are unknown. We now find that Cdk1 serves as a direct master controller for NE dynein recruitment in neural stem cells and HeLa cells. Cdk1 phosphorylates conserved sites within RanBP2 and activates BicD2 binding and early dynein recruitment. Late recruitment is triggered by a Cdk1-induced export of CENP-F from the nucleus. Forced NE targeting of BicD2 overrides Cdk1 inhibition, fully rescuing dynein recruitment and nuclear migration in neural stem cells. These results reveal how NE dynein recruitment is cell cycle regulated, and identify the trigger mechanism for apical nuclear migration in the brain. PMID:26051540

CCDC103 mutations cause primary ciliary dyskinesia by disrupting assembly of ciliary dynein arms

Science.gov (United States)

Panizzi, Jennifer R.; Becker-Heck, Anita; Castleman, Victoria H.; Al-Mutairi, Dalal; Liu, Yan; Loges, Niki T.; Pathak, Narendra; Austin-Tse, Christina; Sheridan, Eamonn; Schmidts, Miriam; Olbrich, Heike; Werner, Claudius; Häffner, Karsten; Hellman, Nathan; Chodhari, Rahul; Gupta, Amar; Kramer-Zucker, Albrecht; Olale, Felix; Burdine, Rebecca D.; Schier, Alexander F.; O’Callaghan, Christopher; Chung, Eddie MK; Reinhardt, Richard; Mitchison, Hannah M.; King, Stephen M.; Omran, Heymut; Drummond, Iain A.

2012-01-01

Cilia are essential for fertilization, respiratory clearance, cerebrospinal fluid circulation, and to establish laterality1. Cilia motility defects cause Primary Ciliary Dyskinesia (PCD, MIM 242650), a disorder affecting 1:15-30,000 births. Cilia motility requires the assembly of multisubunit dynein arms that drive cilia bending2. Despite progress in understanding the genetic basis of PCD, mutations remain to be identified for several PCD linked loci3. Here we show that the zebrafish cilia paralysis mutant schmalhanstn222 (smh) mutant encodes the coiled-coil domain containing 103 protein (Ccdc103), a foxj1a regulated gene. Screening 146 unrelated PCD families identified patients in six families with reduced outer dynein arms, carrying mutations in CCDC103. Dynein arm assembly in smh mutant zebrafish was rescued by wild-type but not mutant human CCDC103. Chlamydomonas Ccdc103 functions as a tightly bound, axoneme-associated protein. The results identify Ccdc103 as a novel dynein arm attachment factor that when mutated causes Primary Ciliary Dyskinesia. PMID:22581229

A splice variant of RILP induces lysosomal clustering independent of dynein recruitment

International Nuclear Information System (INIS)

Marsman, Marije; Jordens, Ingrid; Rocha, Nuno; Kuijl, Coenraad; Janssen, Lennert; Neefjes, Jacques

2006-01-01

The small GTPase Rab7 controls fusion and transport of late endocytic compartments. A critical mediator is the Rab7 effector RILP that recruits the minus-end dynein-dynactin motor complex to these compartments. We identified a natural occurring splice variant of RILP (RILPsv) lacking only 27 amino acids encoded by exon VII. Both variants bind Rab7, prolong its GTP-bound state, and induce clustering of late endocytic compartments. However, RILPsv does not recruit the dynein-dynactin complex, implicating exon VII in motor recruitment. Clustering might still occur via dimerization, since both RILP and RILPsv are able to form hetero- and homo-dimers. Moreover, both effectors compete for Rab7 binding but with different outcome for dynein-dynactin recruitment and transport. Hence, RILPsv provides an extra dimension to the control of vesicle fusion and transport by the small GTPase Rab7

Chlamydomonas DYX1C1/PF23 is essential for axonemal assembly and proper morphology of inner dynein arms.

Directory of Open Access Journals (Sweden)

Ryosuke Yamamoto

2017-09-01

Full Text Available Cytoplasmic assembly of ciliary dyneins, a process known as preassembly, requires numerous non-dynein proteins, but the identities and functions of these proteins are not fully elucidated. Here, we show that the classical Chlamydomonas motility mutant pf23 is defective in the Chlamydomonas homolog of DYX1C1. The pf23 mutant has a 494 bp deletion in the DYX1C1 gene and expresses a shorter DYX1C1 protein in the cytoplasm. Structural analyses, using cryo-ET, reveal that pf23 axonemes lack most of the inner dynein arms. Spectral counting confirms that DYX1C1 is essential for the assembly of the majority of ciliary inner dynein arms (IDA as well as a fraction of the outer dynein arms (ODA. A C-terminal truncation of DYX1C1 shows a reduction in a subset of these ciliary IDAs. Sucrose gradients of cytoplasmic extracts show that preassembled ciliary dyneins are reduced compared to wild-type, which suggests an important role in dynein complex stability. The role of PF23/DYX1C1 remains unknown, but we suggest that DYX1C1 could provide a scaffold for macromolecular assembly.

Chlamydomonas Outer Arm Dynein Alters Conformation in Response to Ca2+

OpenAIRE

Sakato, Miho; Sakakibara, Hitoshi; King, Stephen M.

2007-01-01

We have previously shown that Ca2+ directly activates ATP-sensitive microtubule binding by a Chlamydomonas outer arm dynein subparticle containing the β and γ heavy chains (HCs). The γ HC–associated LC4 light chain is a member of the calmodulin family and binds 1-2 Ca2+ with KCa = 3 × 10−5 M in vitro, suggesting it may act as a Ca2+ sensor for outer arm dynein. Here we investigate interactions between the LC4 light chain and γ HC. Two IQ consensus motifs for binding calmodulin-like proteins a...

Alcohol-induced defects in hepatic transcytosis may be explained by impaired dynein function.

Science.gov (United States)

Groebner, Jennifer L; Fernandez, David J; Tuma, Dean J; Tuma, Pamela L

2014-12-01

Alcoholic liver disease has been clinically well described, but the molecular mechanisms leading to hepatotoxicity have not been fully elucidated. Previously, we determined that microtubules are hyperacetylated and more stable in ethanol-treated WIF-B cells, VL-17A cells, liver slices, and in livers from ethanol-fed rats. From our recent studies, we believe that these modifications can explain alcohol-induced defects in microtubule motor-dependent protein trafficking including nuclear translocation of a subset of transcription factors. Since cytoplasmic dynein/dynactin is known to mediate both microtubule-dependent translocation and basolateral to apical/canalicular transcytosis, we predicted that transcytosis is impaired in ethanol-treated hepatic cells. We monitored transcytosis of three classes of newly synthesized canalicular proteins in polarized, hepatic WIF-B cells, an emerging model system for the study of liver disease. As predicted, canalicular delivery of all proteins tested was impaired in ethanol-treated cells. Unlike in control cells, transcytosing proteins were observed in discrete sub-canalicular puncta en route to the canalicular surface that aligned along acetylated microtubules. We further determined that the stalled transcytosing proteins colocalized with dynein/dynactin in treated cells. No changes in vesicle association were observed for either dynein or dynactin in ethanol-treated cells, but significantly enhanced dynein binding to microtubules was observed. From these results, we propose that enhanced dynein binding to microtubules in ethanol-treated cells leads to decreased motor processivity resulting in vesicle stalling and in impaired canalicular delivery. Our studies also importantly indicate that modulating cellular acetylation levels with clinically tolerated deacetylase agonists may be a novel therapeutic strategy for treating alcoholic liver disease.

Regulation of dynein-mediated autophagosomes trafficking by ASM in CASMCs.

Science.gov (United States)

Xu, Ming; Zhang, Qiufang; Li, Pin-Lan; Nguyen, Thaison; Li, Xiang; Zhang, Yang

2016-01-01

Acid sphingomyelinase (ASM; gene symbol Smpd1) has been shown to play a crucial role in autophagy maturation by controlling lysosomal fusion with autophagosomes in coronary arterial smooth muscle cells (CASMCs). However, the underlying molecular mechanism by which ASM controls autophagolysosomal fusion remains unknown. In primary cultured CASMCs, lysosomal Ca2+ induced by 7-ketocholesterol (7-Ket, an atherogenic stimulus and autophagy inducer) was markedly attenuated by ASM deficiency or TRPML1 gene silencing suggesting that ASM signaling is required for TRPML1 channel activity and subsequent lysosomal Ca(2+) release. In these CASMCs, ASM deficiency or TRPML1 gene silencing markedly inhibited 7-Ket-induced dynein activation. In addition, 7-Ket-induced autophagosome trafficking, an event associated with lysosomal Ca(2+) release and dynein activity, was significantly inhibited in ASM-deficient (Smpd1(-/-)) CASMCs compared to that in Smpd1(+/+) CASMCs. Finally, overexpression of TRPML1 proteins restored 7-Ket-induced lysosomal Ca(2+) release and autophagosome trafficking in Smpd1-/- CASMCs. Collectively, these results suggest that ASM plays a critical role in regulating lysosomal TRPML1-Ca(2+) signaling and subsequent dynein-mediated autophagosome trafficking, which leads its role in controlling autophagy maturation in CASMCs under atherogenic stimulation.

Dynein Light Intermediate Chain 2 Facilitates the Metaphase to Anaphase Transition by Inactivating the Spindle Assembly Checkpoint.

Directory of Open Access Journals (Sweden)

Sagar P Mahale

Full Text Available The multi-functional molecular motor cytoplasmic dynein performs diverse essential roles during mitosis. The mechanistic importance of the dynein Light Intermediate Chain homologs, LIC1 and LIC2 is unappreciated, especially in the context of mitosis. LIC1 and LIC2 are believed to exist in distinct cytoplasmic dynein complexes as obligate subunits. LIC1 had earlier been reported to be required for metaphase to anaphase progression by inactivating the kinetochore-microtubule attachment-sensing arm of the spindle assembly checkpoint (SAC. However, the functional importance of LIC2 during mitosis remains elusive. Here we report prominent novel roles for the LIC2 subunit of cytoplasmic dynein in regulating the spindle assembly checkpoint. LIC2 depletion in mammalian cells led to prolonged metaphase arrest in the presence of an active SAC and also to stretched kinetochores, thus implicating it in SAC inactivation. Quantitative fluorescence microscopy of SAC components revealed accumulation of both attachment- and tension-sensing checkpoint proteins at metaphase kinetochores upon LIC2 depletion. These observations support a stronger and more diverse role in checkpoint inactivation for LIC2 in comparison to its close homolog LIC1. Our study uncovers a novel functional hierarchy during mitotic checkpoint inactivation between the closely related but homologous LIC subunits of cytoplasmic dynein. These subtle functional distinctions between dynein subpopulations could be exploited to study specific aspects of the spindle assembly checkpoint, which is a key mediator of fidelity in eukaryotic cell division.

Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array

NARCIS (Netherlands)

Schuster, M.; Kilaru, S.; Fink, G.; Collemare, J.A.R.; Roger, Y.; Steinberg, G.

2011-01-01

The polarity of microtubules (MTs) determines the motors for intracellular motility, with kinesins moving to plus ends and dynein to minus ends. In elongated cells of Ustilago maydis, dynein is thought to move early endosomes (EEs) toward the septum (retrograde), whereas kinesin-3 transports them to

Chemical and thermal modulation of molecular motor activities

Science.gov (United States)

Hong, Weili

Molecular motors of kinesin and dynein families are responsible for various intracellular activities, from long distance movement of organelles, vesicles, protein complexes, and mRNAs to powering mitotic processes. They can take nanometer steps using chemical energy from the hydrolysis of ATP (adenosine triphosphate), and their dysfunction is involved in many neurodegenerative diseases that require long distance transport of cargos. Here I report on the study of the properties of molecular motors at a single-molecule level using optical trappings. I first studied the inhibition properties of kinesin motors by marine natural compound adociasulfates. I showed that adociasulfates compete with microtubules for binding to kinesins and thus inhibit kinesins' activity. Although adociasulfates are a strong inhibitor for all kinesin members, they show a much higher inhibition effect for conventional kinesins than for mitotic kinesins. Thus adociasulfates can be used to specifically inhibit conventional kinesins. By comparing the inhibition of kinesins by two structurally similar adociasulfates, one can see that the negatively charged sulfate residue of adociasulfates can be replaced by other negative residues and thus make it possible for adociasulfate-derived compounds to be more cell permeable. Kinesins and dyneins move cargos towards opposite directions along a microtubule. Cargos with both kinesins and dyneins attached often move bidirectionally due to undergoing a tug-of-war between the oppositely moving kinesin and dynein motors. Here I studied the effect of temperature on microtubule-based kinesin and dynein motor transport. While kinesins' and dyneins' velocities are closely matched above 15 °C, below this temperature the dyneins' velocity decreases much faster than the kinesins'. The kinesins' and dyneins' forces do not measurably change with temperature. The results suggest that temperature has significant effects on bidirectional transport and can be used to

Antagonism between the dynein and Ndc80 complexes at kinetochores controls the stability of kinetochore-microtubule attachments during mitosis.

Science.gov (United States)

Amin, Mohammed A; McKenney, Richard J; Varma, Dileep

2018-04-20

Chromosome alignment and segregation during mitosis require kinetochore-microtubule (kMT) attachments that are mediated by the molecular motor dynein and the kMT-binding complex Ndc80. The Rod-ZW10-Zwilch (RZZ) complex is central to this coordination as it has an important role in dynein recruitment and has recently been reported to have a key function in the regulation of stable kMT attachments in Caenorhabditis elegans besides its role in activating the spindle assembly checkpoint (SAC). However, the mechanism by which these protein complexes control kMT attachments to drive chromosome motility during early mitosis is still unclear. Here, using in vitro total internal reflection fluorescence microscopy, we observed that higher concentrations of Ndc80 inhibited dynein binding to MTs, providing evidence that Ndc80 and dynein antagonize each other's function. High-resolution microscopy and siRNA-mediated functional disruption revealed that severe defects in chromosome alignment induced by depletion of dynein or the dynein adapter Spindly are rescued by codepletion of the RZZ component Rod in human cells. Interestingly, rescue of the chromosome alignment defects was independent of Rod function in SAC activation and was accompanied by a remarkable restoration of stable kMT attachments. Furthermore, the chromosome alignment rescue depended on the plus-end-directed motility of centromere protein E (CENP-E) because cells codepleted of CENP-E, Rod, and dynein could not establish stable kMT attachments or align their chromosomes properly. Our findings support the idea that dynein may control the function of the Ndc80 complex in stabilizing kMT attachments directly by interfering with Ndc80-MT binding or indirectly by controlling the Rod-mediated inhibition of Ndc80. © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

Optimisation of key performance measures in air cargo demand management

OpenAIRE

Alexander May; Adrian Anslow; Udechukwu Ojiako; Yue Wu; Alasdair Marshall; Maxwell Chipulu

2014-01-01

This article sought to facilitate the optimisation of key performance measures utilised for demand management in air cargo operations. The focus was on the Revenue Management team at Virgin Atlantic Cargo and a fuzzy group decision-making method was used. Utilising intelligent fuzzy multi-criteria methods, the authors generated a ranking order of ten key outcome-based performance indicators for Virgin Atlantic air cargo Revenue Management. The result of this industry-driven study showed that ...

Pick-up, transport and release of a molecular cargo using a small-molecule robotic arm

Science.gov (United States)

Kassem, Salma; Lee, Alan T. L.; Leigh, David A.; Markevicius, Augustinas; Solà, Jordi

2016-02-01

Modern-day factory assembly lines often feature robots that pick up, reposition and connect components in a programmed manner. The idea of manipulating molecular fragments in a similar way has to date only been explored using biological building blocks (specifically DNA). Here, we report on a wholly artificial small-molecule robotic arm capable of selectively transporting a molecular cargo in either direction between two spatially distinct, chemically similar, sites on a molecular platform. The arm picks up/releases a 3-mercaptopropanehydrazide cargo by formation/breakage of a disulfide bond, while dynamic hydrazone chemistry controls the cargo binding to the platform. Transport is controlled by selectively inducing conformational and configurational changes within an embedded hydrazone rotary switch that steers the robotic arm. In a three-stage operation, 79-85% of 3-mercaptopropanehydrazide molecules are transported in either (chosen) direction between the two platform sites, without the cargo at any time fully dissociating from the machine nor exchanging with other molecules in the bulk.

Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes.

Directory of Open Access Journals (Sweden)

Katherine S Ralston

2006-09-01

Full Text Available The Trypanosoma brucei flagellum is a multifunctional organelle with critical roles in motility, cellular morphogenesis, and cell division. Although motility is thought to be important throughout the trypanosome lifecycle, most studies of flagellum structure and function have been restricted to the procyclic lifecycle stage, and our knowledge of the bloodstream form flagellum is limited. We have previously shown that trypanin functions as part of a flagellar dynein regulatory system that transmits regulatory signals from the central pair apparatus and radial spokes to axonemal dyneins. Here we investigate the requirement for this dynein regulatory system in bloodstream form trypanosomes. We demonstrate that trypanin is localized to the flagellum of bloodstream form trypanosomes, in a pattern identical to that seen in procyclic cells. Surprisingly, trypanin RNA interference is lethal in the bloodstream form. These knockdown mutants fail to initiate cytokinesis, but undergo multiple rounds of organelle replication, accumulating multiple flagella, nuclei, kinetoplasts, mitochondria, and flagellum attachment zone structures. These findings suggest that normal flagellar beat is essential in bloodstream form trypanosomes and underscore the emerging concept that there is a dichotomy between trypanosome lifecycle stages with respect to factors that contribute to cell division and cell morphogenesis. This is the first time that a defined dynein regulatory complex has been shown to be essential in any organism and implicates the dynein regulatory complex and other enzymatic regulators of flagellar motility as candidate drug targets for the treatment of African sleeping sickness.

In vivo control mechanisms of motor-cargo movement on microtubules

Science.gov (United States)

Gunawardena, Shermali

2014-03-01

Within axons, molecular motors transport essential components required for neuronal growth and viability. Although many levels of regulation must exist for proper anterograde and retrograde transport of vital proteins, little is known about these mechanisms. Previous work suggested that the amyloid precursor protein (APP) functions as a kinesin-1 receptor during transport. However, how APP vesicle motility is regulated is unclear. Using genetics and in vivo imaging in Drosophila we showed that reduction of presenilin (PS) substantially increased anterograde and retrograde APP vesicle velocities. Strikingly, PS deficiency had no effect on an unrelated cargo vesicle containing synaptotagmin, which is powered by a different kinesin motor. Increased PS-mediated velocities required functional kinesin-1 and dynein motors. We also found that these PS-mediated effects on motor protein function were mediated via a pathway that involves glycogen synthase kinase-3 β (GSK-3 β) . PS genetically interacted with GSK-3 β in an activity dependent manner. Excess of active GSK-3 β perturbed transport by causing axonal blockages, which were enhanced by reduction of kinesin-1 or dynein, while excess of non-functional GSK-3 β had no effect. Strikingly, GSK-3 β-activity dependent transport defects were enhanced by reduction of PS. Collectively, our findings suggest that PS and GSK-3 β are required for normal motor protein function, and we propose a model in which PS likely regulates GSK-3 β activity during transport. These findings have important implications for our understanding of the complex regulatory machinery that must exist in vivo and how this system is coordinated during vesicle motility on microtubules.

WD60/FAP163 is a dynein intermediate chain required for retrograde intraflagellar transport in cilia

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Patel-King, Ramila S.; Gilberti, Renée M.; Hom, Erik F. Y.; King, Stephen M.

2013-01-01

Retrograde intraflagellar transport (IFT) is required for assembly of cilia. We identify a Chlamydomonas flagellar protein (flagellar-associated protein 163 [FAP163]) as being closely related to the D1bIC(FAP133) intermediate chain (IC) of the dynein that powers this movement. Biochemical analysis revealed that FAP163 is present in the flagellar matrix and is actively trafficked by IFT. Furthermore, FAP163 copurified with D1bIC(FAP133) and the LC8 dynein light chain, indicating that it is an integral component of the retrograde IFT dynein. To assess the functional role of FAP163, we generated an RNA interference knockdown of the orthologous protein (WD60) in planaria. The Smed-wd60(RNAi) animals had a severe ciliary assembly defect that dramatically compromised whole-organism motility. Most cilia were present as short stubs that had accumulated large quantities of IFT particle–like material between the doublet microtubules and the membrane. The few remaining approximately full-length cilia had a chaotic beat with a frequency reduced from 24 to ∼10 Hz. Thus WD60/FAP163 is a dynein IC that is absolutely required for retrograde IFT and ciliary assembly. PMID:23864713

Sequential activities of Dynein, Mud and Asp in centrosome-spindle coupling maintain centrosome number upon mitosis.

Science.gov (United States)

Bosveld, Floris; Ainslie, Anna; Bellaïche, Yohanns

2017-10-15

Centrosomes nucleate microtubules and are tightly coupled to the bipolar spindle to ensure genome integrity, cell division orientation and centrosome segregation. While the mechanisms of centrosome-dependent microtubule nucleation and bipolar spindle assembly have been the focus of numerous works, less is known about the mechanisms ensuring the centrosome-spindle coupling. The conserved NuMA protein (Mud in Drosophila ) is best known for its role in spindle orientation. Here, we analyzed the role of Mud and two of its interactors, Asp and Dynein, in the regulation of centrosome numbers in Drosophila epithelial cells. We found that Dynein and Mud mainly initiate centrosome-spindle coupling prior to nuclear envelope breakdown (NEB) by promoting correct centrosome positioning or separation, while Asp acts largely independently of Dynein and Mud to maintain centrosome-spindle coupling. Failure in the centrosome-spindle coupling leads to mis-segregation of the two centrosomes into one daughter cell, resulting in cells with supernumerary centrosomes during subsequent divisions. Altogether, we propose that Dynein, Mud and Asp operate sequentially during the cell cycle to ensure efficient centrosome-spindle coupling in mitosis, thereby preventing centrosome mis-segregation to maintain centrosome number. © 2017. Published by The Company of Biologists Ltd.

Vehicle steering by side stick: optimising steering characteristics

NARCIS (Netherlands)

Zuurbier, J.; Hogema, J.H.; Brekelmans, J.A.W.J.

2000-01-01

This paper describes a study that was conducted to investigate the possibilities for optimizing task performance when driving a side stick-steered vehicle. Using steer-by-wire technology, a conventional steering system was mimicked, thus yielding the possibility to modify the steering

HP-Lattice QSAR for dynein proteins: experimental proteomics (2D-electrophoresis, mass spectrometry) and theoretic study of a Leishmania infantum sequence.

Science.gov (United States)

Dea-Ayuela, María Auxiliadora; Pérez-Castillo, Yunierkis; Meneses-Marcel, Alfredo; Ubeira, Florencio M; Bolas-Fernández, Francisco; Chou, Kuo-Chen; González-Díaz, Humberto

2008-08-15

The toxicity and inefficacy of actual organic drugs against Leishmaniosis justify research projects to find new molecular targets in Leishmania species including Leishmania infantum (L. infantum) and Leishmaniamajor (L. major), both important pathogens. In this sense, quantitative structure-activity relationship (QSAR) methods, which are very useful in Bioorganic and Medicinal Chemistry to discover small-sized drugs, may help to identify not only new drugs but also new drug targets, if we apply them to proteins. Dyneins are important proteins of these parasites governing fundamental processes such as cilia and flagella motion, nuclear migration, organization of the mitotic splinde, and chromosome separation during mitosis. However, despite the interest for them as potential drug targets, so far there has been no report whatsoever on dyneins with QSAR techniques. To the best of our knowledge, we report here the first QSAR for dynein proteins. We used as input the Spectral Moments of a Markov matrix associated to the HP-Lattice Network of the protein sequence. The data contain 411 protein sequences of different species selected by ClustalX to develop a QSAR that correctly discriminates on average between 92.75% and 92.51% of dyneins and other proteins in four different train and cross-validation datasets. We also report a combined experimental and theoretic study of a new dynein sequence in order to illustrate the utility of the model to search for potential drug targets with a practical example. First, we carried out a 2D-electrophoresis analysis of L. infantum biological samples. Next, we excised from 2D-E gels one spot of interest belonging to an unknown protein or protein fragment in the region Mdata base with the highest similarity score to the MS of the protein isolated from L. infantum. We used the QSAR model to predict the new sequence as dynein with probability of 99.99% without relying upon alignment. In order to confirm the previous function annotation we

Three-dimensional reconstruction of axonemal outer dynein arms in situ by electron tomography.

Science.gov (United States)

Lupetti, Pietro; Lanzavecchia, Salvatore; Mercati, David; Cantele, Francesca; Dallai, Romano; Mencarelli, Caterina

2005-10-01

We present here for the first time a 3D reconstruction of in situ axonemal outer dynein arms. This reconstruction has been obtained by electron tomography applied to a series of tilted images collected from metal replicas of rapidly frozen, cryofractured, and metal-replicated sperm axonemes of the cecidomid dipteran Monarthropalpus flavus. This peculiar axonemal model consists of several microtubular laminae that proved to be particularly suitable for this type of analysis. These laminae are sufficiently planar to allow the visualization of many dynein molecules within the same fracture face, allowing us to recover a significant number of equivalent objects and to improve the signal-to-noise ratio of the reconstruction by applying advanced averaging protocols. The 3D model we obtained showed the following interesting structural features: First, each dynein arm has two head domains that are almost parallel and are obliquely oriented with respect to the longitudinal axis of microtubules. The two heads are therefore positioned at different distances from the surface of the A-tubule. Second, each head domain consists of a series of globular subdomains that are positioned on the same plane. Third, a stalk domain originates as a conical region from the proximal head and ends with a small globular domain that contacts the B-tubule. Fourth, the stem region comprises several globular subdomains and presents two distinct points of anchorage to the surface of the A-tubule. Finally, and most importantly, contrary to what has been observed in isolated dynein molecules adsorbed to flat surfaces, the stalk and the stem domains are not in the same plane as the head.

Eclipse Phase of Herpes Simplex Virus Type 1 Infection: Efficient Dynein-Mediated Capsid Transport without the Small Capsid Protein VP26

Science.gov (United States)

Döhner, Katinka; Radtke, Kerstin; Schmidt, Simone; Sodeik, Beate

2006-01-01

Cytoplasmic dynein,together with its cofactor dynactin, transports incoming herpes simplex virus type 1 (HSV-1) capsids along microtubules (MT) to the MT-organizing center (MTOC). From the MTOC, capsids move further to the nuclear pore, where the viral genome is released into the nucleoplasm. The small capsid protein VP26 can interact with the dynein light chains Tctex1 (DYNLT1) and rp3 (DYNLT3) and may recruit dynein to the capsid. Therefore, we analyzed nuclear targeting of incoming HSV1-ΔVP26 capsids devoid of VP26 and of HSV1-GFPVP26 capsids expressing a GFPVP26 fusion instead of VP26. To compare the cell entry of different strains, we characterized the inocula with respect to infectivity, viral genome content, protein composition, and particle composition. Preparations with a low particle-to-PFU ratio showed efficient nuclear targeting and were considered to be of higher quality than those containing many defective particles, which were unable to induce plaque formation. When cells were infected with HSV-1 wild type, HSV1-ΔVP26, or HSV1-GFPVP26, viral capsids were transported along MT to the nucleus. Moreover, when dynein function was inhibited by overexpression of the dynactin subunit dynamitin, fewer capsids of HSV-1 wild type, HSV1-ΔVP26, and HSV1-GFPVP26 arrived at the nucleus. Thus, even in the absence of the potential viral dynein receptor VP26, HSV-1 used MT and dynein for efficient nuclear targeting. These data suggest that besides VP26, HSV-1 encodes other receptors for dynein or dynactin. PMID:16873277

Chlamydomonas outer arm dynein alters conformation in response to Ca2+.

Science.gov (United States)

Sakato, Miho; Sakakibara, Hitoshi; King, Stephen M

2007-09-01

We have previously shown that Ca(2+) directly activates ATP-sensitive microtubule binding by a Chlamydomonas outer arm dynein subparticle containing the beta and gamma heavy chains (HCs). The gamma HC-associated LC4 light chain is a member of the calmodulin family and binds 1-2 Ca(2+) with K(Ca) = 3 x 10(-5) M in vitro, suggesting it may act as a Ca(2+) sensor for outer arm dynein. Here we investigate interactions between the LC4 light chain and gamma HC. Two IQ consensus motifs for binding calmodulin-like proteins are located within the stem domain of the gamma heavy chain. In vitro experiments indicate that LC4 undergoes a Ca(2+)-dependent interaction with the IQ motif domain while remaining tethered to the HC. LC4 also moves into close proximity of the intermediate chain IC1 in the presence of Ca(2+). The sedimentation profile of the gamma HC subunit changed subtly upon Ca(2+) addition, suggesting that the entire complex had become more compact, and electron microscopy of the isolated gamma subunit revealed a distinct alteration in conformation of the N-terminal stem in response to Ca(2+) addition. We propose that Ca(2+)-dependent conformational change of LC4 has a direct effect on the stem domain of the gamma HC, which eventually leads to alterations in mechanochemical interactions between microtubules and the motor domain(s) of the outer dynein arm.

46 CFR 154.534 - Cargo pumps and cargo compressors.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo pumps and cargo compressors. 154.534 Section 154.534 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES... Equipment Cargo and Process Piping Systems § 154.534 Cargo pumps and cargo compressors. Cargo pumps and...

S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner

Science.gov (United States)

Grallert, Agnes; Beuter, Christoph; Craven, Rachel A.; Bagley, Steve; Wilks, Deepti; Fleig, Ursula; Hagan, Iain M.

2006-01-01

The Schizosaccharomyces pombe CLIP170-associated protein (CLASP) Peg1 was identified in a screen for mutants with spindle formation defects and a screen for molecules that antagonized EB1 function. The conditional peg1.1 mutant enabled us to identify key features of Peg1 function. First, Peg1 was required to form a spindle and astral microtubules, yet destabilized interphase microtubules. Second, Peg1 was required to slow the polymerization rate of interphase microtubules that establish end-on contact with the cortex at cell tips. Third, Peg1 antagonized the action of S. pombe CLIP170 (Tip1) and EB1 (Mal3). Fourth, although Peg1 resembled higher eukaryotic CLASPs by physically associating with both Mal3 and Tip1, neither Tip1 nor Mal3 was required for Peg1 to destabilize interphase microtubules or for it to associate with microtubules. Conversely, neither Mal3 nor Tip1 required Peg1 to associate with microtubules or cell tips. Consistently, while mal3.Δ and tip1.Δ disrupted linear growth, corrupting peg1 + did not. Fifth, peg1.1 phenotypes resembled those arising from deletion of the single heavy or both light chains of fission yeast dynein. Furthermore, all interphase phenotypes arising from peg1 + manipulation relied on dynein function. Thus, the impact of S. pombe CLASP on interphase microtubule behavior is more closely aligned to dynein than EB1 or CLIP170. PMID:16951255

A mutation in the dynein heavy chain gene compensates for energy deficit of mutant SOD1 mice and increases potentially neuroprotective IGF-1

Directory of Open Access Journals (Sweden)

Larmet Yves

2011-04-01

Full Text Available Abstract Background Amyotrophic lateral sclerosis (ALS is a fatal neurodegenerative disease characterized by a progressive loss of motor neurons. ALS patients, as well as animal models such as mice overexpressing mutant SOD1s, are characterized by increased energy expenditure. In mice, this hypermetabolism leads to energy deficit and precipitates motor neuron degeneration. Recent studies have shown that mutations in the gene encoding the dynein heavy chain protein are able to extend lifespan of mutant SOD1 mice. It remains unknown whether the protection offered by these dynein mutations relies on a compensation of energy metabolism defects. Results SOD1(G93A mice were crossbred with mice harboring the dynein mutant Cramping allele (Cra/+ mice. Dynein mutation increased adipose stores in compound transgenic mice through increasing carbohydrate oxidation and sparing lipids. Metabolic changes that occurred in double transgenic mice were accompanied by the normalization of the expression of key mRNAs in the white adipose tissue and liver. Furthermore, Dynein Cra mutation rescued decreased post-prandial plasma triglycerides and decreased non esterified fatty acids upon fasting. In SOD1(G93A mice, the dynein Cra mutation led to increased expression of IGF-1 in the liver, increased systemic IGF-1 and, most importantly, to increased spinal IGF-1 levels that are potentially neuroprotective. Conclusions These findings suggest that the protection against SOD1(G93A offered by the Cramping mutation in the dynein gene is, at least partially, mediated by a reversal in energy deficit and increased IGF-1 availability to motor neurons.

Optimisation of key performance measures in air cargo demand management

Directory of Open Access Journals (Sweden)

Alexander May

2014-04-01

Full Text Available This article sought to facilitate the optimisation of key performance measures utilised for demand management in air cargo operations. The focus was on the Revenue Management team at Virgin Atlantic Cargo and a fuzzy group decision-making method was used. Utilising intelligent fuzzy multi-criteria methods, the authors generated a ranking order of ten key outcome-based performance indicators for Virgin Atlantic air cargo Revenue Management. The result of this industry-driven study showed that for Air Cargo Revenue Management, ‘Network Optimisation’ represents a critical outcome-based performance indicator. This collaborative study contributes to existing logistics management literature, especially in the area of Revenue Management, and it seeks to enhance Revenue Management practice. It also provides a platform for Air Cargo operators seeking to improve reliability values for their key performance indicators as a means of enhancing operational monitoring power.

Spatial relationships and movement patterns of the air cargo industry in airport regions

Directory of Open Access Journals (Sweden)

Petrus J. van V. Coetzee

2017-05-01

Full Text Available Background: During the past few years, with the increase in air traffic and the expansion of airports, very few industries had such a large spatial development and movement impact as that of airport-related clusters or airport regions. Although much research was done on the various impacts of the airport industry, very little research was done on the air cargo industry in airport regions. Objectives: This article specifically explored the unique spatial relationships, impacts, trends and movement patterns of the air cargo industry within a typical airport region. Method: The article focused on the OR Tambo International Airport in Gauteng, South Africa, as a case study and was informed by an extensive quantitative spatial and land use analysis and modelling of the study area. Results: The article presented findings and insights on the movement patterns and relationships between (1 the airport facility and (2 the spatial configuration of air cargo industries in the particular airport region. These findings also provided some framework for a possible spatial model and guideline that could assist in steering and managing development and movement patterns in airport regions. Conclusion: The article provided new insights and understanding on the spatial dynamics of airport regions and the air cargo industry, ultimately addressing some gaps in this knowledge field. The article in the end highlighted the need for a different and novel approach to the planning and management of the air cargo industry in airport regions and a basis for further research.

Resonance Assignments and Secondary Structure Analysis of Dynein Light Chain 8 by Magic-angle Spinning NMR Spectroscopy

Energy Technology Data Exchange (ETDEWEB)

Sun, Shangjin; Butterworth, Andrew H.; Paramasivam, Sivakumar; Yan, Si; Lightcap, Christine M.; Williams, John C.; Polenova, Tatyana E.

2011-08-04

Dynein light chain LC8 is the smallest subunit of the dynein motor complex and has been shown to play important roles in both dynein-dependent and dynein-independent physiological functions via its interaction with a number of its binding partners. It has also been linked to pathogenesis including roles in viral infections and tumorigenesis. Structural information for LC8-target proteins is critical to understanding the underlying function of LC8 in these complexes. However, some LC8-target interactions are not amenable to structural characterization by conventional structural biology techniques owing to their large size, low solubility, and crystallization difficulties. Here, we report magic-angle spinning (MAS) NMR studies of the homodimeric apo-LC8 protein as a first effort in addressing more complex, multi-partner, LC8-based protein assemblies. We have established site-specific backbone and side-chain resonance assignments for the majority of the residues of LC8, and show TALOS+-predicted torsion angles ø and ψ in close agreement with most residues in the published LC8 crystal structure. Data obtained through these studies will provide the first step toward using MAS NMR to examine the LC8 structure, which will eventually be used to investigate protein–protein interactions in larger systems that cannot be determined by conventional structural studies.

Tongue-driven sonar beam steering by a lingual-echolocating fruit bat.

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Wu-Jung Lee

2017-12-01

Full Text Available Animals enhance sensory acquisition from a specific direction by movements of head, ears, or eyes. As active sensing animals, echolocating bats also aim their directional sonar beam to selectively "illuminate" a confined volume of space, facilitating efficient information processing by reducing echo interference and clutter. Such sonar beam control is generally achieved by head movements or shape changes of the sound-emitting mouth or nose. However, lingual-echolocating Egyptian fruit bats, Rousettus aegyptiacus, which produce sound by clicking their tongue, can dramatically change beam direction at very short temporal intervals without visible morphological changes. The mechanism supporting this capability has remained a mystery. Here, we measured signals from free-flying Egyptian fruit bats and discovered a systematic angular sweep of beam focus across increasing frequency. This unusual signal structure has not been observed in other animals and cannot be explained by the conventional and widely-used "piston model" that describes the emission pattern of other bat species. Through modeling, we show that the observed beam features can be captured by an array of tongue-driven sound sources located along the side of the mouth, and that the sonar beam direction can be steered parsimoniously by inducing changes to the pattern of phase differences through moving tongue location. The effects are broadly similar to those found in a phased array-an engineering design widely found in human-made sonar systems that enables beam direction changes without changes in the physical transducer assembly. Our study reveals an intriguing parallel between biology and human engineering in solving problems in fundamentally similar ways.

One-way EPR steering and genuine multipartite EPR steering

Science.gov (United States)

He, Qiongyi; Reid, Margaret D.

2012-11-01

We propose criteria and experimental strategies to realise the Einstein-Podolsky-Rosen (EPR) steering nonlocality. One-way steering can be obtained where there is asymmetry of thermal noise on each system. We also present EPR steering inequalities that act as signatures and suggest how to optimise EPR correlations in specific schemes so that the genuine multipartite EPR steering nonlocality (EPR paradox) can also possibly be realised. The results presented here also apply to the spatially separated macroscopic atomic ensembles.

Expansion and Polarity Sorting in Microtubule-Dynein Bundles(WHAT IS LIFE? THE NEXT 100 YEARS OF YUKAWA'S DREAM)

OpenAIRE

Assaf, ZEMEL; Alex, MOGILNER; Department of Neurobiology, Physiology and Behavior, University of California; Department of Neurobiology, Physiology and Behavior, University of California

2008-01-01

Interactions of multiple molecular motors with dynamic polymers, such as actin and microtubules, form the basis for many processes in the cell cytoskeleton. One example is the active 'sorting' of microtubule bundles by dynein molecular motors into aster-like arrays of microtubules; in these bundles dynein motors cross-link and slide neighboring microtubules apart. A number of models have been suggested to quantify the active dynamics of cross-linked bundles of polar filaments. In the case of ...

Design of power steering systems for heavy-duty long-haul vehicles

NARCIS (Netherlands)

Silvas, E.; Backx, E.A.; Hofman, T.; Voets, H.; Steinbuch, M.

2014-01-01

Conventionally, all auxiliaries present in a heavy-duty vehicle (e.g., power-steering pump, air-conditioning compressor) are engine-driven systems, which put high constraints on their performance. Outputs (e.g., speed, temperature) and energy consumption are dictated by engine speed, while most

Dynein Heavy Chain, Encoded by Two Genes in Agaricomycetes, Is Required for Nuclear Migration in Schizophyllum commune.

Directory of Open Access Journals (Sweden)

Melanie Brunsch

Full Text Available The white-rot fungus Schizophyllum commune (Agaricomycetes was used to study the cell biology of microtubular trafficking during mating interactions, when the two partners exchange nuclei, which are transported along microtubule tracks. For this transport activity, the motor protein dynein is required. In S. commune, the dynein heavy chain is encoded in two parts by two separate genes, dhc1 and dhc2. The N-terminal protein Dhc1 supplies the dimerization domain, while Dhc2 encodes the motor machinery and the microtubule binding domain. This split motor protein is unique to Basidiomycota, where three different sequence patterns suggest independent split events during evolution. To investigate the function of the dynein heavy chain, the gene dhc1 and the motor domain in dhc2 were deleted. Both resulting mutants were viable, but revealed phenotypes in hyphal growth morphology and mating behavior as well as in sexual development. Viability of strain Δdhc2 is due to the higher expression of kinesin-2 and kinesin-14, which was proven via RNA sequencing.

Steering Maps and Their Application to Dimension-Bounded Steering

Science.gov (United States)

Moroder, Tobias; Gittsovich, Oleg; Huber, Marcus; Uola, Roope; Gühne, Otfried

2016-03-01

The existence of quantum correlations that allow one party to steer the quantum state of another party is a counterintuitive quantum effect that was described at the beginning of the past century. Steering occurs if entanglement can be proven even though the description of the measurements on one party is not known, while the other side is characterized. We introduce the concept of steering maps, which allow us to unlock sophisticated techniques that were developed in regular entanglement detection and to use them for certifying steerability. As an application, we show that this allows us to go beyond even the canonical steering scenario; it enables a generalized dimension-bounded steering where one only assumes the Hilbert space dimension on the characterized side, with no description of the measurements. Surprisingly, this does not weaken the detection strength of very symmetric scenarios that have recently been carried out in experiments.

Altered steering strategies for goal-directed locomotion in stroke

Science.gov (United States)

2013-01-01

Background Individuals who have sustained a stroke can manifest altered locomotor steering behaviors when exposed to optic flows expanding from different locations. Whether these alterations persist in the presence of a visible goal and whether they can be explained by the presence of a perceptuo-motor disorder remain unknown. The purpose of this study was to compare stroke participants and healthy participants on their ability to control heading while exposed to changing optic flows and target locations. Methods Ten participants with stroke (55.6 ± 9.3 yrs) and ten healthy controls (57.0 ± 11.5 yrs) participated in a mouse-driven steering task (perceptuo-motor task) while seated and in a walking steering task. In the seated steering task, participants were instructed to head or ‘walk’ toward a target in the virtual environment by using a mouse while wearing a helmet-mounted display (HMD). In the walking task, participants performed a similar steering task in the same virtual environment while walking overground at their comfortable speed. For both experiments, the target and/or the focus of expansion (FOE) of the optic flow shifted to the side (±20°) or remained centered. The main outcome measure was net heading errors (NHE). Secondary outcomes included mediolateral displacement, horizontal head orientation, and onsets of heading and head reorientation. Results In the walking steering task, the presence of FOE shifts modulated the extent and timing of mediolateral displacement and head rotation changes, as well as NHE magnitudes. Participants overshot and undershot their net heading, respectively, in response to ipsilateral and contralateral FOE and target shifts. Stroke participants made larger NHEs, especially when the FOE was shifted towards the non-paretic side. In the seated steering task, similar NHEs were observed between stroke and healthy participants. Conclusions The findings highlight the fine coordination between rotational and

Accelerator requirements for fast-neutron interrogation of luggage and cargo

International Nuclear Information System (INIS)

Micklich, B.J.; Fink, C.L.; Yule, T.J.

1995-01-01

Several different fast-neutron based techniques are being studied for the detection of contraband substances in luggage and cargo containers. The present work discusses the accelerator requirements for fast-neutron transmission spectroscopy (FNTS), pulsed fast-neutron analysis (PFNA), and 14-MeV neutron interrogation. These requirements are based on the results of Monte-Carlo simulations of neutron or gamma detection rates. Accelerator requirements are driven by count-rate considerations, spatial resolution and acceptable uncertainties in elemental compositions. The authors have limited their analyses to luggage inspection with FNTS and to cargo inspection with PFNA or 14-MeV neutron interrogation

Distribution of tubulin, kinesin, and dynein in light- and dark-adapted octopus retinas.

Science.gov (United States)

Martinez, J M; Elfarissi, H; De Velasco, B; Ochoa, G H; Miller, A M; Clark, Y M; Matsumoto, B; Robles, L J

2000-01-01

Cephalopod retinas exhibit several responses to light and dark adaptation, including rhabdom size changes, photopigment movements, and pigment granule migration. Light- and dark-directed rearrangements of microfilament and microtubule cytoskeletal transport pathways could drive these changes. Recently, we localized actin-binding proteins in light-/dark-adapted octopus rhabdoms and suggested that actin cytoskeletal rearrangements bring about the formation and degradation of rhabdomere microvilli subsets. To determine if the microtubule cytoskeleton and associated motor proteins control the other light/dark changes, we used immunoblotting and immunocytochemical procedures to map the distribution of tubulin, kinesin, and dynein in dorsal and ventral halves of light- and dark-adapted octopus retinas. Immunoblots detected alpha- and beta-tubulin, dynein intermediate chain, and kinesin heavy chain in extracts of whole retinas. Epifluorescence and confocal microscopy showed that the tubulin proteins were distributed throughout the retina with more immunoreactivity in retinas exposed to light. Kinesin localization was heavy in the pigment layer of light- and dark-adapted ventral retinas but was less prominent in the dorsal region. Dynein distribution also varied in dorsal and ventral retinas with more immunoreactivity in light- and dark-adapted ventral retinas and confocal microscopy emphasized the granular nature of this labeling. We suggest that light may regulate the distribution of microtubule cytoskeletal proteins in the octopus retina and that position, dorsal versus ventral, also influences the distribution of motor proteins. The microtubule cytoskeleton is most likely involved in pigment granule migration in the light and dark and with the movement of transport vesicles from the photoreceptor inner segments to the rhabdoms.

Mutations in ZMYND10, a gene essential for proper axonemal assembly of inner and outer dynein arms in humans and flies, cause primary ciliary dyskinesia

DEFF Research Database (Denmark)

Moore, Daniel J; Onoufriadis, Alexandros; Shoemark, Amelia

2013-01-01

Primary ciliary dyskinesia (PCD) is a ciliopathy characterized by airway disease, infertility, and laterality defects, often caused by dual loss of the inner dynein arms (IDAs) and outer dynein arms (ODAs), which power cilia and flagella beating. Using whole-exome and candidate-gene Sanger resequ...

Integrated proteomics identified novel activation of dynein IC2-GR-COX-1 signaling in neurofibromatosis type I (NF1) disease model cells.

Science.gov (United States)

Hirayama, Mio; Kobayashi, Daiki; Mizuguchi, Souhei; Morikawa, Takashi; Nagayama, Megumi; Midorikawa, Uichi; Wilson, Masayo M; Nambu, Akiko N; Yoshizawa, Akiyasu C; Kawano, Shin; Araki, Norie

2013-05-01

Neurofibromatosis type 1 (NF1) tumor suppressor gene product, neurofibromin, functions in part as a Ras-GAP, and though its loss is implicated in the neuronal abnormality of NF1 patients, its precise cellular function remains unclear. To study the molecular mechanism of NF1 pathogenesis, we prepared NF1 gene knockdown (KD) PC12 cells, as a NF1 disease model, and analyzed their molecular (gene and protein) expression profiles with a unique integrated proteomics approach, comprising iTRAQ, 2D-DIGE, and DNA microarrays, using an integrated protein and gene expression analysis chart (iPEACH). In NF1-KD PC12 cells showing abnormal neuronal differentiation after NGF treatment, of 3198 molecules quantitatively identified and listed in iPEACH, 97 molecules continuously up- or down-regulated over time were extracted. Pathway and network analysis further revealed overrepresentation of calcium signaling and transcriptional regulation by glucocorticoid receptor (GR) in the up-regulated protein set, whereas nerve system development was overrepresented in the down-regulated protein set. The novel up-regulated network we discovered, "dynein IC2-GR-COX-1 signaling," was then examined in NF1-KD cells. Validation studies confirmed that NF1 knockdown induces altered splicing and phosphorylation patterns of dynein IC2 isomers, up-regulation and accumulation of nuclear GR, and increased COX-1 expression in NGF-treated cells. Moreover, the neurite retraction phenotype observed in NF1-KD cells was significantly recovered by knockdown of the dynein IC2-C isoform and COX-1. In addition, dynein IC2 siRNA significantly inhibited nuclear translocation and accumulation of GR and up-regulation of COX-1 expression. These results suggest that dynein IC2 up-regulates GR nuclear translocation and accumulation, and subsequently causes increased COX-1 expression, in this NF1 disease model. Our integrated proteomics strategy, which combines multiple approaches, demonstrates that NF1-related neural

Control of intravascular catheters using an array of active steering coils.

Science.gov (United States)

Gudino, N; Heilman, J A; Derakhshan, J J; Sunshine, J L; Duerk, J L; Griswold, M A

2011-07-01

To extend the concept of deflecting the tip of a catheter with the magnetic force created in an MRI system through the use of an array of independently controllable steering coils located in the catheter tip, and to present methods for visualization of the catheter and/or surrounding areas while the catheter is deflected. An array of steering coils made of 42-gauge wire was built over a 2.5 Fr (0.83 mm) fiber braided microcatheter. Two of the coils were 70 turn axial coils separated by 1 cm, and the third was a 15-turn square side coil that was 2 x 4 mm2. Each coil was driven independently by a pulse width modulation (PWM) current source controlled by a microprocessor that received commands from a MATLAB routine that dynamically set current amplitude and direction for each coil. The catheter was immersed in a water phantom containing 1% Gd-DTPA that was placed at the isocenter of a 1.5 T MRI scanner. Deflections of the catheter tip were measured from image-based data obtained with a real-time radio frequency (RF) spoiled gradient echo sequence (GRE). The small local magnetic fields generated by the steering coils were exploited to generate a hyperintense signal at the catheter tip by using a modified GRE sequence that did not include slice-select rewinding gradients. Imaging and excitation modes were implemented by synchronizing the excitation of the steering coil array with the scanner by ensuring that no current was driven through the coils during the data acquisition window; this allowed visualization of the surrounding tissue while not affecting the desired catheter position. Deflections as large as 2.5 cm were measured when exciting the steering coils sequentially with a 100 mA maximum current per coil. When exciting a single axial coil, the deflection was half this value with 30% higher current. A hyperintense catheter tip useful for catheter tracking was obtained by imaging with the modified GRE sequence. Clear visualization of the areas surrounding the

Online optimized hysteresis-based steering feel model for steer-by-wire systems

Directory of Open Access Journals (Sweden)

Ahmet Kirli

2016-06-01

Full Text Available In rubber-wheeled road vehicles, the mechanical connection between steering wheel and front wheels provides steering-related feedback to the driver. The torque fed back to the driver through the steering linkages and steering wheel, which is called steering feel, helps the driver in controlling the vehicle. The torque feedback is reproduced via artificial methods in steer-by-wire systems due to the lack of mechanical connection. In this work, in order to minimize the physical workload and the lateral acceleration under the consideration of handling performance, optimization of a hysteresis-based steering feel has been studied. A 2-degree-of-freedom bicycle model based on the magic formula tire model has been used for simulations and hardware-in-the-loop experiments. A mathematical model is proposed in order to create an adaptive model-based optimization of the hysteresis parameters simultaneously while driving. A hardware-in-the-loop experimental setup has been used for the driving tests. The weave and the double-lane change tests have been performed with different drivers in order to demonstrate and quantify the optimization methods that are presented in this work.

A new model to compute the desired steering torque for steer-by-wire vehicles and driving simulators

Science.gov (United States)

Fankem, Steve; Müller, Steffen

2014-05-01

This paper deals with the control of the hand wheel actuator in steer-by-wire (SbW) vehicles and driving simulators (DSs). A novel model for the computation of the desired steering torque is presented. The introduced steering torque computation does not only aim to generate a realistic steering feel, which means that the driver should not miss the basic steering functionality of a modern conventional steering system such as an electric power steering (EPS) or hydraulic power steering (HPS), and this in every driving situation. In addition, the modular structure of the steering torque computation combined with suitably selected tuning parameters has the objective to offer a high degree of customisability of the steering feel and thus to provide each driver with his preferred steering feel in a very intuitive manner. The task and the tuning of each module are firstly described. Then, the steering torque computation is parameterised such that the steering feel of a series EPS system is reproduced. For this purpose, experiments are conducted in a hardware-in-the-loop environment where a test EPS is mounted on a steering test bench coupled with a vehicle simulator and parameter identification techniques are applied. Subsequently, how appropriate the steering torque computation mimics the test EPS system is objectively evaluated with respect to criteria concerning the steering torque level and gradient, the feedback behaviour and the steering return ability. Finally, the intuitive tuning of the modular steering torque computation is demonstrated for deriving a sportier steering feel configuration.

A MATHEMATICAL MODEL OF THE MILITARY TRANSPORT AIRCRAFT MOVEMENT AT CARGO ITEM DROP

Directory of Open Access Journals (Sweden)

2016-01-01

Full Text Available The controllability of military transport aircraft deteriorates at heavy single piece landing. To solve this problem and a specific methodology for pilotage of the pre-emption, and automation tools are being developed. Preliminary study ofpilotage technique and authomatic control algorythm demand a reliable mathematical model of aircraft dynamics at cargo item drop. Such model should take into account significant change in the position of the aircraft center of mass and aircraft inertia tensor. Simplified models were based on modeling the movement of the center of mass and rotation around the cen- ter of mass of the aircraft. Such models do not take into account the inertial forces and moments of moving a cargo item. This circumstance does not allow to obtain reliable results in the simulation. The article presents the description of the complete mathematical model of the movement of military transport aircraft in landing of a cargo item. Examines the com- plex material system of solids and a detailed description of the properties of its components. The equations of motion of the aircraft as a system carrier (aircraft without a cargo item and wear (of moving a cargo item bodies to reflect the changes in the inertia tensor. The functioning of the power plant, steering actuators, flight control system, an exhaust chute, the sen- sors of the primary information are taken into account. The equations of motion for systems of bodies projected on the air- craft reference plane are being recorded. This approach takes into account changes of the inertia tensor and the position of the main central axes of inertia in the process of landing of a cargo item. It allows us to simulate the condition of the air- craft at all speeds of the pitch, normal overload, and masses of single piece and placement, as evidenced by the high con- vergence of modeling results with data from flight tests.

Random intermittent search and the tug-of-war model of motor-driven transport

International Nuclear Information System (INIS)

Newby, Jay; Bressloff, Paul C

2010-01-01

We formulate the 'tug-of-war' model of microtubule cargo transport by multiple molecular motors as an intermittent random search for a hidden target. A motor complex consisting of multiple molecular motors with opposing directional preference is modeled using a discrete Markov process. The motors randomly pull each other off of the microtubule so that the state of the motor complex is determined by the number of bound motors. The tug-of-war model prescribes the state transition rates and corresponding cargo velocities in terms of experimentally measured physical parameters. We add space to the resulting Chapman–Kolmogorov (CK) equation so that we can consider delivery of the cargo to a hidden target at an unknown location along the microtubule track. The target represents some subcellular compartment such as a synapse in a neuron's dendrites, and target delivery is modeled as a simple absorption process. Using a quasi-steady-state (QSS) reduction technique we calculate analytical approximations of the mean first passage time (MFPT) to find the target. We show that there exists an optimal adenosine triphosphate (ATP) concentration that minimizes the MFPT for two different cases: (i) the motor complex is composed of equal numbers of kinesin motors bound to two different microtubules (symmetric tug-of-war model) and (ii) the motor complex is composed of different numbers of kinesin and dynein motors bound to a single microtubule (asymmetric tug-of-war model)

Random intermittent search and the tug-of-war model of motor-driven transport

Science.gov (United States)

Newby, Jay; Bressloff, Paul C.

2010-04-01

We formulate the 'tug-of-war' model of microtubule cargo transport by multiple molecular motors as an intermittent random search for a hidden target. A motor complex consisting of multiple molecular motors with opposing directional preference is modeled using a discrete Markov process. The motors randomly pull each other off of the microtubule so that the state of the motor complex is determined by the number of bound motors. The tug-of-war model prescribes the state transition rates and corresponding cargo velocities in terms of experimentally measured physical parameters. We add space to the resulting Chapman-Kolmogorov (CK) equation so that we can consider delivery of the cargo to a hidden target at an unknown location along the microtubule track. The target represents some subcellular compartment such as a synapse in a neuron's dendrites, and target delivery is modeled as a simple absorption process. Using a quasi-steady-state (QSS) reduction technique we calculate analytical approximations of the mean first passage time (MFPT) to find the target. We show that there exists an optimal adenosine triphosphate (ATP) concentration that minimizes the MFPT for two different cases: (i) the motor complex is composed of equal numbers of kinesin motors bound to two different microtubules (symmetric tug-of-war model) and (ii) the motor complex is composed of different numbers of kinesin and dynein motors bound to a single microtubule (asymmetric tug-of-war model).

Random intermittent search and the tug-of-war model of motor-driven transport

KAUST Repository

Newby, Jay

2010-04-16

We formulate the \\'tug-of-war\\' model of microtubule cargo transport by multiple molecular motors as an intermittent random search for a hidden target. A motor complex consisting of multiple molecular motors with opposing directional preference is modeled using a discrete Markov process. The motors randomly pull each other off of the microtubule so that the state of the motor complex is determined by the number of bound motors. The tug-of-war model prescribes the state transition rates and corresponding cargo velocities in terms of experimentally measured physical parameters. We add space to the resulting Chapman-Kolmogorov (CK) equation so that we can consider delivery of the cargo to a hidden target at an unknown location along the microtubule track. The target represents some subcellular compartment such as a synapse in a neuron\\'s dendrites, and target delivery is modeled as a simple absorption process. Using a quasi-steady-state (QSS) reduction technique we calculate analytical approximations of the mean first passage time (MFPT) to find the target. We show that there exists an optimal adenosine triphosphate (ATP) concentration that minimizes the MFPT for two different cases: (i) the motor complex is composed of equal numbers of kinesin motors bound to two different microtubules (symmetric tug-of-war model) and (ii) the motor complex is composed of different numbers of kinesin and dynein motors bound to a single microtubule (asymmetric tug-of-war model). © 2010 IOP Publishing Ltd.

Random intermittent search and the tug-of-war model of motor-driven transport

KAUST Repository

Newby, Jay; Bressloff, Paul C

2010-01-01

We formulate the 'tug-of-war' model of microtubule cargo transport by multiple molecular motors as an intermittent random search for a hidden target. A motor complex consisting of multiple molecular motors with opposing directional preference is modeled using a discrete Markov process. The motors randomly pull each other off of the microtubule so that the state of the motor complex is determined by the number of bound motors. The tug-of-war model prescribes the state transition rates and corresponding cargo velocities in terms of experimentally measured physical parameters. We add space to the resulting Chapman-Kolmogorov (CK) equation so that we can consider delivery of the cargo to a hidden target at an unknown location along the microtubule track. The target represents some subcellular compartment such as a synapse in a neuron's dendrites, and target delivery is modeled as a simple absorption process. Using a quasi-steady-state (QSS) reduction technique we calculate analytical approximations of the mean first passage time (MFPT) to find the target. We show that there exists an optimal adenosine triphosphate (ATP) concentration that minimizes the MFPT for two different cases: (i) the motor complex is composed of equal numbers of kinesin motors bound to two different microtubules (symmetric tug-of-war model) and (ii) the motor complex is composed of different numbers of kinesin and dynein motors bound to a single microtubule (asymmetric tug-of-war model). © 2010 IOP Publishing Ltd.

On Electrohydraulic Pressure Control for Power Steering Applications : Active Steering for Road Vehicles

OpenAIRE

Dell'Amico, Alessandro

2016-01-01

This thesis deals with the Electrohydraulic Power Steering system for road vehicles, using electronic pressure control valves. With an ever increasing demand for safer vehicles and fewer traffic accidents, steering-related active safety functions are becoming more common in modern vehicles. Future road vehicles will also evolve towards autonomous vehicles, with several safety, environmental and financial benefits. A key component in realising such solutions is active steering. The power steer...

49 CFR 570.7 - Steering systems.

Science.gov (United States)

2010-10-01

... 49 Transportation 6 2010-10-01 2010-10-01 false Steering systems. 570.7 Section 570.7... Pounds or Less § 570.7 Steering systems. (a) System play. Lash or free play in the steering system shall... in the steering system. Table 1—Steering System Free Play Values Steering wheel diameter (inches...

Kinesin and Dynein Mechanics: Measurement Methods and Research Applications.

Science.gov (United States)

Abraham, Zachary; Hawley, Emma; Hayosh, Daniel; Webster-Wood, Victoria A; Akkus, Ozan

2018-02-01

Motor proteins play critical roles in the normal function of cells and proper development of organisms. Among motor proteins, failings in the normal function of two types of proteins, kinesin and dynein, have been shown to lead many pathologies, including neurodegenerative diseases and cancers. As such, it is critical to researchers to understand the underlying mechanics and behaviors of these proteins, not only to shed light on how failures may lead to disease, but also to guide research toward novel treatment and nano-engineering solutions. To this end, many experimental techniques have been developed to measure the force and motility capabilities of these proteins. This review will (a) discuss such techniques, specifically microscopy, atomic force microscopy (AFM), optical trapping, and magnetic tweezers, and (b) the resulting nanomechanical properties of motor protein functions such as stalling force, velocity, and dependence on adenosine triphosophate (ATP) concentrations will be comparatively discussed. Additionally, this review will highlight the clinical importance of these proteins. Furthermore, as the understanding of the structure and function of motor proteins improves, novel applications are emerging in the field. Specifically, researchers have begun to modify the structure of existing proteins, thereby engineering novel elements to alter and improve native motor protein function, or even allow the motor proteins to perform entirely new tasks as parts of nanomachines. Kinesin and dynein are vital elements for the proper function of cells. While many exciting experiments have shed light on their function, mechanics, and applications, additional research is needed to completely understand their behavior.

Experimental verification of multidimensional quantum steering

Science.gov (United States)

Li, Che-Ming; Lo, Hsin-Pin; Chen, Liang-Yu; Yabushita, Atsushi

2018-03-01

Quantum steering enables one party to communicate with another remote party even if the sender is untrusted. Such characteristics of quantum systems not only provide direct applications to quantum information science, but are also conceptually important for distinguishing between quantum and classical resources. While concrete illustrations of steering have been shown in several experiments, quantum steering has not been certified for higher dimensional systems. Here, we introduce a simple method to experimentally certify two different kinds of quantum steering: Einstein-Podolsky-Rosen (EPR) steering and single-system (SS) steering (i.e., temporal steering), for dimensionality (d) up to d = 16. The former reveals the steerability among bipartite systems, whereas the latter manifests itself in single quantum objects. We use multidimensional steering witnesses to verify EPR steering of polarization-entangled pairs and SS steering of single photons. The ratios between the measured witnesses and the maximum values achieved by classical mimicries are observed to increase with d for both EPR and SS steering. The designed scenario offers a new method to study further the genuine multipartite steering of large dimensionality and potential uses in quantum information processing.

Finding the Cell Center by a Balance of Dynein and Myosin Pulling and Microtubule Pushing: A Computational Study

Science.gov (United States)

Zhu, Jie; Burakov, Anton; Rodionov, Vladimir

2010-01-01

The centrosome position in many types of interphase cells is actively maintained in the cell center. Our previous work indicated that the centrosome is kept at the center by pulling force generated by dynein and actin flow produced by myosin contraction and that an unidentified factor that depends on microtubule dynamics destabilizes position of the centrosome. Here, we use modeling to simulate the centrosome positioning based on the idea that the balance of three forces—dyneins pulling along microtubule length, myosin-powered centripetal drag, and microtubules pushing on organelles—is responsible for the centrosome displacement. By comparing numerical predictions with centrosome behavior in wild-type and perturbed interphase cells, we rule out several plausible hypotheses about the nature of the microtubule-based force. We conclude that strong dynein- and weaker myosin-generated forces pull the microtubules inward competing with microtubule plus-ends pushing the microtubule aster outward and that the balance of these forces positions the centrosome at the cell center. The model also predicts that kinesin action could be another outward-pushing force. Simulations demonstrate that the force-balance centering mechanism is robust yet versatile. We use the experimental observations to reverse engineer the characteristic forces and centrosome mobility. PMID:20980619

Inhibition of microtubules and dynein rescues human immunodeficiency virus type 1 from owl monkey TRIMCyp-mediated restriction in a cellular context-specific fashion.

Science.gov (United States)

Pawlica, Paulina; Dufour, Caroline; Berthoux, Lionel

2015-04-01

IFN-induced restriction factors can significantly affect the replicative capacity of retroviruses in mammals. TRIM5α (tripartite motif protein 5, isoform α) is a restriction factor that acts at early stages of the virus life cycle by intercepting and destabilizing incoming retroviral cores. Sensitivity to TRIM5α maps to the N-terminal domain of the retroviral capsid proteins. In several New World and Old World monkey species, independent events of retrotransposon-mediated insertion of the cyclophilin A (CypA)-coding sequence in the trim5 gene have given rise to TRIMCyp (also called TRIM5-CypA), a hybrid protein that is active against some lentiviruses in a species-specific fashion. In particular, TRIMCyp from the owl monkey (omkTRIMCyp) very efficiently inhibits human immunodeficiency virus type 1 (HIV-1). Previously, we showed that disrupting the integrity of microtubules (MTs) and of cytoplasmic dynein complexes partially rescued replication of retroviruses, including HIV-1, from restriction mediated by TRIM5α. Here, we showed that efficient restriction of HIV-1 by omkTRIMCyp was similarly dependent on the MT network and on dynein complexes, but in a context-dependent fashion. When omkTRIMCyp was expressed in human HeLa cells, restriction was partially counteracted by pharmacological agents targeting MTs or by small interfering RNA-mediated inhibition of dynein. The same drugs (nocodazole and paclitaxel) also rescued HIV-1 from restriction in cat CRFK cells, although to a lesser extent. Strikingly, neither nocodazole, paclitaxel nor depletion of the dynein heavy chain had a significant effect on the restriction of HIV-1 in an owl monkey cell line. These results suggested the existence of cell-specific functional interactions between MTs/dynein and TRIMCyp. © 2015 The Authors.

Characterization of a subunit of the outer dynein arm docking complex necessary for correct flagellar assembly in Leishmania donovani.

Directory of Open Access Journals (Sweden)

Simone Harder

Full Text Available BACKGROUND: In order to proceed through their life cycle, Leishmania parasites switch between sandflies and mammals. The flagellated promastigote cells transmitted by the insect vector are phagocytized by macrophages within the mammalian host and convert into the amastigote stage, which possesses a rudimentary flagellum only. During an earlier proteomic study of the stage differentiation of the parasite we identified a component of the outer dynein arm docking complex, a structure of the flagellar axoneme. The 70 kDa subunit of the outer dynein arm docking complex consists of three subunits altogether and is essential for the assembly of the outer dynein arm onto the doublet microtubule of the flagella. According to the nomenclature of the well-studied Chlamydomonas reinhardtii complex we named the Leishmania protein LdDC2. METHODOLOGY/PRINCIPAL FINDINGS: This study features a characterization of the protein over the life cycle of the parasite. It is synthesized exclusively in the promastigote stage and localizes to the flagellum. Gene replacement mutants of lddc2 show reduced growth rates and diminished flagellar length. Additionally, the normally spindle-shaped promastigote parasites reveal a more spherical cell shape giving them an amastigote-like appearance. The mutants lose their motility and wiggle in place. Ultrastructural analyses reveal that the outer dynein arm is missing. Furthermore, expression of the amastigote-specific A2 gene family was detected in the deletion mutants in the absence of a stage conversion stimulus. In vitro infectivity is slightly increased in the mutant cell line compared to wild-type Leishmania donovani parasites. CONCLUSIONS/SIGNIFICANCE: Our results indicate that the correct assembly of the flagellum has a great influence on the investigated characteristics of Leishmania parasites. The lack of a single flagellar protein causes an aberrant morphology, impaired growth and altered infectiousness of the parasite.

Research on Precision Tracking on Fast Steering Mirror and Control Strategy

Science.gov (United States)

Di, Lin; Yi-ming, Wu; Fan, Zhu

2018-01-01

Fast steering mirror is a device used for controlling the beam direction precisely. Due to the short travel of the push-pull FSM, a compound fast steering mirror system driven by both limited-angle voice coil motor and push-pull FSM together is proposed. In the compound FSM system, limited-angle voice coil motor quickly swings at wide angle, while the push-pull FSM do high frequency movement in a small range, which provides the system with the high bandwidth and long travel. In the control strategy, the method of combining feed-forward control in Kalman filtering with auto-disturbance rejection control is used to improve trajectory tracking accuracy. The simulation result shows that tracking accuracy measured by the compound method can be improved by more than 5 times than that of the conventional PID.

Two-degrees-of-freedom piezo-driven fast steering mirror with cross-axis decoupling capability

Science.gov (United States)

Shao, Shubao; Tian, Zheng; Song, Siyang; Xu, Minglong

2018-05-01

Because mechanical cross coupling between its axes would lead to degradation of the scanning precision of a piezo-driven fast steering mirror (PFSM), a two-degrees-of-freedom (2-DoF) PFSM with a cross-axis decoupling capability, in which 2-DoF flexure hinges are used, is proposed in this work. The overall structure of the proposed PFSM is first introduced and then both static and dynamic models are established analytically; in addition, the decoupling mechanism is described in detail and the low dynamic cross coupling ratios that occur between the two DoFs are shown. Because of the decoupling property of the PFSM, the 2-DoF motion is treated as a combination of two independent one-degree-of-freedom (1-DoF) motions and two independent proportional-integral-derivative controllers are thus used separately in the control of the two DoFs. Based on this control strategy, experiments involving both 1-DoF trajectory tracking and 2-DoF trajectory tracking are implemented. The test results show that the proposed PFSM can achieve the tilt range of ±7 mrad for both axes with the low coupling ratios that are less than 2% (-34 dB), and the bandwidths of both axes are higher than 810 Hz; in addition, the maximal tracking full scale range errors for 1-DoF trajectory tracking and 2-DoF trajectory tracking are less than 0.2% and 1%, respectively, where the larger error of 2-DoF trajectory tracking is mainly caused by the remaining cross coupling between axes.

Driver steering model for closed-loop steering function analysis

Science.gov (United States)

Bolia, Pratiksh; Weiskircher, Thomas; Müller, Steffen

2014-05-01

In this paper, a two level preview driver steering control model for the use in numerical vehicle dynamics simulation is introduced. The proposed model is composed of cascaded control loops: The outer loop is the path following layer based on potential field framework. The inner loop tries to capture the driver's physical behaviour. The proposed driver model allows easy implementation of different driving situations to simulate a wide range of different driver types, moods and vehicle types. The expediency of the proposed driver model is shown with the help of developed driver steering assist (DSA) function integrated with a conventional series production (Electric Power steering System with rack assist servo unit) system. With the help of the DSA assist function, the driver is prevented from over saturating the front tyre forces and loss of stability and controllability during cornering. The simulation results show different driver reactions caused by the change in the parameters or properties of the proposed driver model if the DSA assist function is activated. Thus, the proposed driver model is useful for the advanced driver steering and vehicle stability assist function evaluation in the early stage of vehicle dynamics handling and stability evaluation.

Study on control schemes of flexible steering system of a multi-axle all-wheel-steering robot

Directory of Open Access Journals (Sweden)

Pingxia Zhang

2016-05-01

Full Text Available It is well known that a multi-axle wheeled robot possesses larger load capability and also higher drive performance. However, its steering flexibility is degraded due to the large number of wheels. In order to solve this problem, in this article, we proposed three control schemes based on the center of rotation or the steering angles of both the first- and last-axle wheels. To release these control schemes, steering mode selection and also the left wheel’s steering angle in a specific axle are added approaching a practical application. Thereafter, the remaining wheels’ steering angles can be calculated with the Ackerman steering theorem. In order to verify the control effects, a five-axle all-wheel-steering wheeled robot has been developed with the Bluetooth wireless monitor system. Based on the newly designed robot, validation experiments are carried out, such as lateral movement, situ rotation, and multi-mode steering within a narrow space. The results indicate that the proposed design in this article can ensure a more flexible and faster movement within a narrow space. It shows large potential in obstacle avoidance compared with the conventional partial-wheel steering mode.

Steering smog prediction

NARCIS (Netherlands)

R. van Liere (Robert); J.J. van Wijk (Jack)

1997-01-01

textabstractThe use of computational steering for smog prediction is described. This application is representative for many underlying issues found in steering high performance applications: high computing times, large data sets, and many different input parameters. After a short description of the

Phase control of entanglement and quantum steering in a three-mode optomechanical system

Science.gov (United States)

Sun, F. X.; Mao, D.; Dai, Y. T.; Ficek, Z.; He, Q. Y.; Gong, Q. H.

2017-12-01

The theory of phase control of coherence, entanglement and quantum steering is developed for an optomechanical system composed of a single mode cavity containing a partially transmitting dielectric membrane and driven by short laser pulses. The membrane divides the cavity into two mutually coupled optomechanical cavities resulting in an effective three-mode closed loop system, two field modes of the two cavities and a mechanical mode representing the oscillating membrane. The closed loop in the coupling creates interfering channels which depend on the relative phase of the coupling strengths of the field modes to the mechanical mode. Populations and correlations of the output modes are calculated analytically and show several interesting phase dependent effects such as reversible population transfer from one field mode to the other, creation of collective modes, and induced coherence without induced emission. We find that these effects result from perfect mutual coherence between the field modes which is preserved even if one of the modes is not populated. The inseparability criterion for the output modes is also investigated and we find that entanglement may occur only between the field modes and the mechanical mode. We show that depending on the phase, the field modes can act on the mechanical mode collectively or individually resulting, respectively, in tripartite or bipartite entanglement. In addition, we examine the phase sensitivity of quantum steering of the mechanical mode by the field modes. Deterministic phase transfer of the steering from bipartite to collective is predicted and optimum steering corresponding to perfect EPR state can be achieved. These different types of quantum steering can be distinguished experimentally by measuring the coincidence rate between two detectors adjusted to collect photons of the output cavity modes. In particular, we find that the minima of the interference pattern of the coincidence rate signal the bipartite steering

A Frictionless Steering Mechanism for the Front Steering ECCD ITER Upper Port Launcher

International Nuclear Information System (INIS)

Chavan, R; Henderson, M A; Sanchez, F

2005-01-01

A FS launcher is being designed for the ITER upper port, which offers enhanced physics performance over the RS launcher. A two mirror system is used to decouple the focusing and steering aspects of the launcher and provide a relatively small beam waist ( 1.6 m from the steering mirror). The resulting NTM stabilization efficiency (maximum CD density divided by the local bootstrap current >1.6) is above marginal for the q = 2 and 3/2 rational flux surfaces of the relevant ITER equilibria (scenarios 2, 3a and 5) and a factor of ∼3 relative to an equivalent RS launcher. The performance of the FS launcher strongly depends on the reliability of the steering mechanism, which is used to rotate the plasma facing steering mirror. CRPP has designed a frictionless steering mechanism assembled in a compact cartridge capable of up to ±10 deg. rotation (corresponding to a poloidal steering range of up to ±20 deg. for the microwave beam around a fixed axis of rotation) that offers a high operation reliability despite the close proximity to the thermal and neutron flux coming from the ITER plasma

Tissue Specific Roles of Dynein Light Chain 1 in Regulating Germ Cell Apoptosis in Ceanorhabditis elegans

DEFF Research Database (Denmark)

Morthorst, Tine Hørning

2015-01-01

in the etiology of many diseases, including cancer, neurodegenerative, cardiovascular and autoimmune diseases. Several of the first genes found to regulate apoptosis were discovered in the nematode Caenorhabditis elegans. In this project, two different and tissue specific roles of C. elegans dynein light chain 1...

Wisconsin Air Cargo Study

Science.gov (United States)

2011-04-01

Air cargo is a key economic lifeline for the communities that have airports. Manufacturers, businesses, hospitals and : other community cornerstone employers depend on air cargo to successfully operate. While there is no doubt that air : cargo repres...

Excitation Patterns of Standard and Steered Partial Tripolar Stimuli in Cochlear Implants.

Science.gov (United States)

Wu, Ching-Chih; Luo, Xin

2016-04-01

Current steering in partial tripolar (pTP) mode has been shown to improve pitch perception and spectral resolution with cochlear implants (CIs). In this mode, a fraction (σ) of the main electrode current is returned within the cochlea and steered between the basal and apical flanking electrodes (with a proportion of α and 1 - α, respectively). Pitch generally decreases when α increases from 0 to 1, although the salience of pitch change varies across CI users. This study aimed to identify the mechanism of pitch changes with pTP-mode current steering and the factors contributing to the intersubject variability in pitch-ranking sensitivity. The electrical fields were measured for steered pTP stimuli on the same main electrode with α = 0, 0.5, and 1 in five implanted ears using electrical field imaging (EFI). The related excitation patterns were also measured physiologically using evoked compound action potential (ECAP) and psychophysically using psychophysical forward masking (PFM). Consistent with the pitch-ranking results in this study, the EFI, ECAP, and PFM centroids shifted apically with increasing α. An apical shift was also observed for the PFM peak but not for the EFI or ECAP peak. The pattern width was similar with different α values within a given measure (e.g., EFI, ECAP, or PFM), but the ECAP patterns were broader than the EFI and PFM patterns, possibly because ECAP was measured with smaller σ values than EFI and PFM. The amount of pattern shift with α depended on σ (i.e., the total amount of current used for steering) but was not correlated with the pitch-ranking sensitivity across subjects. The results revealed that the pitch changes elicited by pTP-mode current steering were not only driven by the shifts of excitation centroid.

Networks: structure and action : steering in and steering by policy networks

NARCIS (Netherlands)

Dassen, A.

2010-01-01

This thesis explores the opportunities to build a structural policy network model that is rooted in social network theories. By making a distinction between a process of steering in networks, and a process of steering by networks, it addresses the effects of network structures on network dynamics as

Steering handbook

CERN Document Server

Pfeffer, Peter

2017-01-01

This edited volume presents basic principles as well as advanced concepts of the computational modeling of steering systems. Moreover, the book includes the components and functionalities of modern steering system, which are presented comprehensively and in a practical way. The book is written by more than 15 leading experts from the automotive industry and its components suppliers. The target audience primarily comprises practicing engineers, developers, researchers as well as graduate students who want to specialize in this field.

Improvement of the directional stability of passenger car trailer couplings with actively controlled steering

Science.gov (United States)

Desens, Jens

The stabilization of pendulum oscillations of passenger car trailer couplings, using active steering, was examined. A linear model of the couplings was presented. Each axle was provided with a controller. The controllers were optimized, with regard to necessary sensors, in order to minimize costs. The rear and the front axles were provided with a control unit in order to compute the potential prevailing in the active steering of several axles. It was shown that the passenger car rear axle was the most suitable for coupling stabilization. The experiment was simulated, using a complex coupling model. The developed controller allowed the passenger car trailer to be driven at a speed higher than 150 km per hour.

46 CFR 182.610 - Main steering gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Main steering gear. 182.610 Section 182.610 Shipping...) MACHINERY INSTALLATION Steering Systems § 182.610 Main steering gear. (a) A vessel must be provided with a main steering gear that is: (1) Of adequate strength and capable of steering the vessel at all service...

Quantum steering in cascaded four-wave mixing processes.

Science.gov (United States)

Wang, Li; Lv, Shuchao; Jing, Jietai

2017-07-24

Quantum steering is used to describe the "spooky action-at-a-distance" nonlocality raised in the Einstein-Podolsky-Rosen (EPR) paradox, which is important for understanding entanglement distribution and constructing quantum networks. Here, in this paper, we study an experimentally feasible scheme for generating quantum steering based on cascaded four-wave-mixing (FWM) processes in hot rubidium (Rb) vapor. Quantum steering, including bipartite steering and genuine tripartite steering among the output light fields, is theoretically analyzed. We find the corresponding gain regions in which the bipartite and tripartite steering exist. The results of bipartite steering can be used to establish a hierarchical steering model in which one beam can steer the other two beams in the whole gain region; however, the other two beams cannot steer the first beam simultaneously. Moreover, the other two beams cannot steer with each other in the whole gain region. More importantly, we investigate the gain dependence of the existence of the genuine tripartite steering and we find that the genuine tripartite steering exists in most of the whole gain region in the ideal case. Also we discuss the effect of losses on the genuine tripartite steering. Our results pave the way to experimental demonstration of quantum steering in cascaded FWM process.

46 CFR 153.907 - Cargo information.

Science.gov (United States)

2010-10-01

... Information § 153.907 Cargo information. (a) The master shall ensure that the following information for each cargo carried under this part is readily available to those on the tankship engaged in cargo operations... exposed to the cargo. (7) A list of fire fighting procedures and extinguishing agents effective with cargo...

Multiobjective optimization of a steering linkage

Energy Technology Data Exchange (ETDEWEB)

Sleesonsom, S.; Bureerat, S. [Sustainable and Infrastructure Research and Development Center, Dept. of Mechanical Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen (Thailand)

2016-08-15

In this paper, multi-objective optimization of a rack-and-pinion steering linkage is proposed. This steering linkage is a common mechanism used in small cars with three advantages as it is simple to construct, economical to manufacture, and compact and easy to operate. In the previous works, many researchers tried to minimize a steering error but minimization of a turning radius is somewhat ignored. As a result, a multi-objective optimization problem is assigned to simultaneously minimize a steering error and a turning radius. The design variables are linkage dimensions. The design problem is solved by the hybrid of multi-objective population-based incremental learning and differential evolution with various constraint handling schemes. The new design strategy leads to effective design of rack-and-pinion steering linkages satisfying both steering error and turning radius criteria.

Multiobjective optimization of a steering linkage

International Nuclear Information System (INIS)

Sleesonsom, S.; Bureerat, S.

2016-01-01

In this paper, multi-objective optimization of a rack-and-pinion steering linkage is proposed. This steering linkage is a common mechanism used in small cars with three advantages as it is simple to construct, economical to manufacture, and compact and easy to operate. In the previous works, many researchers tried to minimize a steering error but minimization of a turning radius is somewhat ignored. As a result, a multi-objective optimization problem is assigned to simultaneously minimize a steering error and a turning radius. The design variables are linkage dimensions. The design problem is solved by the hybrid of multi-objective population-based incremental learning and differential evolution with various constraint handling schemes. The new design strategy leads to effective design of rack-and-pinion steering linkages satisfying both steering error and turning radius criteria

Steering and evasion assist

NARCIS (Netherlands)

Dang, T.; Desens, J.; Franke, U.; Gavrila, D.; Schäfers, L.; Ziegler, W.; Eskandarian, A.

2012-01-01

Steering and evasion assistance defines a new and future class of driver assistance systems to avoid an impending collision with other traffic participants. Dynamic and kinematic considerations reveal that an evasive steering maneuver has high potential for collision avoidance in many driving

Improvement of motor inertia influence of electric power steering; Dendoshiki power steering no motor kansei no eikyo to hosho

Energy Technology Data Exchange (ETDEWEB)

Takehara, S; Sakamoto, K; Hanamoto, Y [Mazda Motor Corp., Hiroshima (Japan); Noritsugu, T [Okayama University, Okayama (Japan)

1997-10-01

Motor inertia of electric power steering affects not only steering characteristics but vehicle dynamics. We have investigated the influence of motor inertia and proposed a feedback strategy to compensate it. Weight of the test vehicle is 1100Kg and the steering system is pinion type electric power steering. By using simulation model and vehicle test, we have realized natural steering maneuvering and stable vehicle dynamics. 4 refs., 11 figs.

Probing the Energetics of Dynactin Filament Assembly and the Binding of Cargo Adaptor Proteins Using Molecular Dynamics Simulation and Electrostatics-Based Structural Modeling.

Science.gov (United States)

Zheng, Wenjun

2017-01-10

Dynactin, a large multiprotein complex, binds with the cytoplasmic dynein-1 motor and various adaptor proteins to allow recruitment and transportation of cellular cargoes toward the minus end of microtubules. The structure of the dynactin complex is built around an actin-like minifilament with a defined length, which has been visualized in a high-resolution structure of the dynactin filament determined by cryo-electron microscopy (cryo-EM). To understand the energetic basis of dynactin filament assembly, we used molecular dynamics simulation to probe the intersubunit interactions among the actin-like proteins, various capping proteins, and four extended regions of the dynactin shoulder. Our simulations revealed stronger intersubunit interactions at the barbed and pointed ends of the filament and involving the extended regions (compared with the interactions within the filament), which may energetically drive filament termination by the capping proteins and recruitment of the actin-like proteins by the extended regions, two key features of the dynactin filament assembly process. Next, we modeled the unknown binding configuration among dynactin, dynein tails, and a number of coiled-coil adaptor proteins (including several Bicaudal-D and related proteins and three HOOK proteins), and predicted a key set of charged residues involved in their electrostatic interactions. Our modeling is consistent with previous findings of conserved regions, functional sites, and disease mutations in the adaptor proteins and will provide a structural framework for future functional and mutational studies of these adaptor proteins. In sum, this study yielded rich structural and energetic information about dynactin and associated adaptor proteins that cannot be directly obtained from the cryo-EM structures with limited resolutions.

Demand side management using profile steering

NARCIS (Netherlands)

Gerards, Marco Egbertus Theodorus; Toersche, Hermen; Hoogsteen, Gerwin; van der Klauw, Thijs; Hurink, Johann L.; Smit, Gerardus Johannes Maria

2015-01-01

Many Demand Side Management (DSM) approaches use energy prices as steering signals. This paper shows that such steering signals may result in power quality problems and high losses. As an alternative, this paper proposes to use desired (e.g., flat) power profiles as steering signals and presents an

Experimental temporal quantum steering

Czech Academy of Sciences Publication Activity Database

Bartkiewicz, K.; Černoch, Antonín; Lemr, K.; Miranowicz, A.; Nori, F.

2016-01-01

Roč. 6, Nov (2016), 1-8, č. článku 38076. ISSN 2045-2322 R&D Projects: GA ČR GAP205/12/0382 Institutional support: RVO:68378271 Keywords : temporal quantum steering * EPR steering Subject RIV: BH - Optics, Masers, Lasers Impact factor: 4.259, year: 2016

Einstein-Podolsky-Rosen paradox and quantum steering in a three-mode optomechanical system

Science.gov (United States)

He, Qiongyi; Ficek, Zbigniew

2014-02-01

We study multipartite entanglement, the generation of Einstein-Podolsky-Rosen (EPR) states, and quantum steering in a three-mode optomechanical system composed of an atomic ensemble located inside a single-mode cavity with a movable mirror. The cavity mode is driven by a short laser pulse, has a nonlinear parametric-type interaction with the mirror and a linear beam-splitter-type interaction with the atomic ensemble. There is no direct interaction of the mirror with the atomic ensemble. A threshold effect for the dynamics of the system is found, above which the system works as an amplifier and below which as an attenuator of the output fields. The threshold is determined by the ratio of the coupling strengths of the cavity mode to the mirror and to the atomic ensemble. It is shown that above the threshold, the system effectively behaves as a two-mode system in which a perfect bipartite EPR state can be generated, while it is impossible below the threshold. Furthermore, a fully inseparable tripartite entanglement and even further a genuine tripartite entanglement can be produced above and below the threshold. In addition, we consider quantum steering and examine the monogamy relations that quantify the amount of bipartite steering that can be shared between different modes. It is found that the mirror is more capable for steering of entanglement than the cavity mode. The two-way steering is found between the mirror and the atomic ensemble despite the fact that they are not directly coupled to each other, while it is impossible between the output of cavity mode and the ensemble which are directly coupled to each other.

46 CFR 176.814 - Steering systems.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Steering systems. 176.814 Section 176.814 Shipping COAST...) INSPECTION AND CERTIFICATION Material Inspections § 176.814 Steering systems. At each initial and subsequent inspection for certification the owner or managing operator shall be prepared to test the steering systems of...

Beam Steering Devices Reduce Payload Weight

Science.gov (United States)

2012-01-01

Scientists have long been able to shift the direction of a laser beam, steering it toward a target, but often the strength and focus of the light is altered. For precision applications, where the quality of the beam cannot be compromised, scientists have typically turned to mechanical steering methods, redirecting the source of the beam by swinging the entire laser apparatus toward the target. Just as the mechanical methods used for turning cars has evolved into simpler, lighter, power steering methods, so has the means by which researchers can direct lasers. Some of the typical contraptions used to redirect lasers are large and bulky, relying on steering gimbals pivoted, rotating supports to shift the device toward its intended target. These devices, some as large and awkward as a piece of heavy luggage, are subject to the same issues confronted by mechanical parts: Components rub, wear out, and get stuck. The poor reliability and bulk not to mention the power requirements to run one of the machines have made mechanical beam steering components less than ideal for use in applications where weight, bulk, and maneuverability are prime concerns, such as on an unmanned aerial vehicle (UAV) or a microscope. The solution to developing reliable, lighter weight, nonmechanical steering methods to replace the hefty steering boxes was to think outside the box, and a NASA research partner did just that by developing a new beam steering method that bends and redirects the beam, as opposed to shifting the entire apparatus. The benefits include lower power requirements, a smaller footprint, reduced weight, and better control and flexibility in steering capabilities. Such benefits are realized without sacrificing aperture size, efficiency, or scanning range, and can be applied to myriad uses: propulsion systems, structures, radiation protection systems, and landing systems.

Modeling and simulations of new electrostatically driven, bimorph actuator for high beam steering micromirror deflection angles

Science.gov (United States)

Walton, John P.; Coutu, Ronald A.; Starman, LaVern

2015-02-01

There are numerous applications for micromirror arrays seen in our everyday lives. From flat screen televisions and computer monitors, found in nearly every home and office, to advanced military weapon systems and space vehicles, each application bringing with it a unique set of requirements. The microelectromechanical systems (MEMS) industry has researched many ways micromirror actuation can be accomplished and the different constraints on performance each design brings with it. This paper investigates a new "zipper" approach to electrostatically driven micromirrors with the intent of improving duel plane beam steering by coupling large deflection angles, over 30°, and a fast switching speed. To accomplish this, an extreme initial deflection is needed which can be reached using high stress bimorph beams. Currently this requires long beams and high voltage for the electrostatic pull in or slower electrothermal switching. The idea for this "zipper" approach is to stack multiple beams of a much shorter length and allow for the deflection of each beam to be added together in order to reach the required initial deflection height. This design requires much less pull-in voltage because the pull-in of one short beam will in turn reduce the height of the all subsequent beams, making it much easier to actuate. Using modeling and simulation software to characterize operations characteristics, different bimorph cantilever beam configurations are explored in order to optimize the design. These simulations show that this new "zipper" approach increases initial deflection as additional beams are added to the assembly without increasing the actuation voltage.

Operator space approach to steering inequality

International Nuclear Information System (INIS)

Yin, Zhi; Marciniak, Marcin; Horodecki, Michał

2015-01-01

In Junge and Palazuelos (2011 Commun. Math. Phys. 306 695–746) and Junge et al (2010 Commun. Math. Phys. 300 715–39) the operator space theory was applied to study bipartite Bell inequalities. The aim of the paper is to follow this line of research and use the operator space technique to analyze the steering scenario. We obtain a bipartite steering functional with unbounded largest violation of steering inequality, as well as constructing all ingredients explicitly. It turns out that the unbounded largest violation is obtained by a non maximally entangled state. Moreover, we focus on the bipartite dichotomic case where we construct a steering functional with unbounded largest violation of steering inequality. This phenomenon is different to the Bell scenario where only the bounded largest violation can be obtained by any bipartite dichotomic Bell functional. (paper)

29 CFR 1918.84 - Bulling cargo.

Science.gov (United States)

2010-07-01

... 29 Labor 7 2010-07-01 2010-07-01 false Bulling cargo. 1918.84 Section 1918.84 Labor Regulations...) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Handling Cargo § 1918.84 Bulling cargo. (a) Bulling cargo shall be done with the bull line led directly from the heel block. However, bulling may be done from the...

The p25 Subunit of the Dynactin Complex is Required for Dynein-Early Endosome Interaction

Science.gov (United States)

2011-01-01

early endosome movement. In filamentous hyphae , dynein powers the minus end–directed movement of early endosomes (Steinberg and Schuster 2011...observed in time-lapse sequences (Video 1; Abenza et al., 2009). In still images, early endosomes were seen to distribute along the hyphae (Fig. 2 A...nuclear distribution along elongated hyphae and also for the microtubule minus end–directed movement of early endosomes away from the tip (Morris

46 CFR 154.235 - Cargo tank location.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank location. 154.235 Section 154.235 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY STANDARDS... Survival Capability and Cargo Tank Location § 154.235 Cargo tank location. (a) For type IG hulls, cargo...

Driver behavior following an automatic steering intervention.

Science.gov (United States)

Fricke, Nicola; Griesche, Stefan; Schieben, Anna; Hesse, Tobias; Baumann, Martin

2015-10-01

The study investigated driver behavior toward an automatic steering intervention of a collision mitigation system. Forty participants were tested in a driving simulator and confronted with an inevitable collision. They performed a naïve drive and afterwards a repeated exposure in which they were told to hold the steering wheel loosely. In a third drive they experienced a false alarm situation. Data on driving behavior, i.e. steering and braking behavior as well as subjective data was assessed in the scenarios. Results showed that most participants held on to the steering wheel strongly or counter-steered during the system intervention during the first encounter. Moreover, subjective data collected after the first drive showed that the majority of drivers was not aware of the system intervention. Data from the repeated drive in which participants were instructed to hold the steering wheel loosely, led to significantly more participants holding the steering wheel loosely and thus complying with the instruction. This study seems to imply that without knowledge and information of the system about an upcoming intervention, the most prevalent driving behavior is a strong reaction with the steering wheel similar to an automatic steering reflex which decreases the system's effectiveness. Results of the second drive show some potential for countermeasures, such as informing drivers shortly before a system intervention in order to prevent inhibiting reactions. Copyright © 2015 Elsevier Ltd. All rights reserved.

Biogas production from steer manures in Vietnam

DEFF Research Database (Denmark)

Pham, Cuong H.; Saggar, Surinder; Vu, Cuong C.

2017-01-01

manures collected from two different experiments of steers fed diets containing feed supplements. BMP was 110.1 (NLkg-1 VS) for manure from steers receiving a control diet, significantly lower 79.0 (NL kg-1 VS) for manure from steers fed a diet containing 0.3% tannin (%DM), but then showed an increasing...... trend to 90.9 and 91.2 (NL kg-1 VS) for manures from steers receiving 0.4 and 0.5% tannin (%DM) supplements, respectively. Similarly, the CH4 production (NL kg-1 VS) of manure from steers was 174 for control, 142 for control supplemented concentrate (C), 143 for control added rice straw treated...

A cargo-sorting DNA robot.

Science.gov (United States)

Thubagere, Anupama J; Li, Wei; Johnson, Robert F; Chen, Zibo; Doroudi, Shayan; Lee, Yae Lim; Izatt, Gregory; Wittman, Sarah; Srinivas, Niranjan; Woods, Damien; Winfree, Erik; Qian, Lulu

2017-09-15

Two critical challenges in the design and synthesis of molecular robots are modularity and algorithm simplicity. We demonstrate three modular building blocks for a DNA robot that performs cargo sorting at the molecular level. A simple algorithm encoding recognition between cargos and their destinations allows for a simple robot design: a single-stranded DNA with one leg and two foot domains for walking, and one arm and one hand domain for picking up and dropping off cargos. The robot explores a two-dimensional testing ground on the surface of DNA origami, picks up multiple cargos of two types that are initially at unordered locations, and delivers them to specified destinations until all molecules are sorted into two distinct piles. The robot is designed to perform a random walk without any energy supply. Exploiting this feature, a single robot can repeatedly sort multiple cargos. Localization on DNA origami allows for distinct cargo-sorting tasks to take place simultaneously in one test tube or for multiple robots to collectively perform the same task. Copyright © 2017, American Association for the Advancement of Science.

Active and passive transport of cargo in a corrugated channel: A lattice model study

Science.gov (United States)

Dey, Supravat; Ching, Kevin; Das, Moumita

2018-04-01

Inside cells, cargos such as vesicles and organelles are transported by molecular motors to their correct locations via active motion on cytoskeletal tracks and passive, Brownian diffusion. During the transportation of cargos, motor-cargo complexes (MCCs) navigate the confining and crowded environment of the cytoskeletal network and other macromolecules. Motivated by this, we study a minimal two-state model of motor-driven cargo transport in confinement and predict transport properties that can be tested in experiments. We assume that the motion of the MCC is directly affected by the entropic barrier due to confinement if it is in the passive, unbound state but not in the active, bound state where it moves with a constant bound velocity. We construct a lattice model based on a Fokker Planck description of the two-state system, study it using a kinetic Monte Carlo method and compare our numerical results with analytical expressions for a mean field limit. We find that the effect of confinement strongly depends on the bound velocity and the binding kinetics of the MCC. Confinement effectively reduces the effective diffusivity and average velocity, except when it results in an enhanced average binding rate and thereby leads to a larger average velocity than when unconfined.

Fundamentals studies of a magnetically steered vacuum arc

Science.gov (United States)

Walke, Paul

In recent years demand from production industry for high performance cutting tools, aero and automobile engine parts has prompted research into both existing and novel methods of laying down hard, low friction coatings . A key process for the production of such coatings has been Physical Vapour Deposition (PVD) which has proved to be a consistent and reliable tool for industry. For this technique to continue to be improved and more advanced coatings to be produced, research at the fundamental level is required. This thesis describes research investigating the behaviour of the steered arc cathode spot and methods of improving existing steered arc coating technology.The majority of existing steered arc systems use either permanent magnets or a combination of permanent and electromagnets to steer the arc. Described here is a novel system which employs a pair of electromagnetic coils of cylindrical geometry which enable the arc to be positioned on a circular orbit through a range of continuously variable radii. In addition to this the coils are capable of controlling the transverse and normal magnetic field profiles independently of the steering radius selected. This enables the behaviour of the arc spot to be investigated under a range of magnetic field conditions thus allowing the comparison of measured arc behaviour with a new model of arc motion. Care has described the motion of the arc spot as a biased random walk and has derived an analytical solution to describe the time dependent, probability density function for the arc position in two dimensions. Two distributions are proposed (one in each dimension); the first describes the probability density for the arc position in the direction of driven motion, the second the probability density in the direction of arc confinement. The shape of these distributions is dependent upon the transverse and normal components of the applied magnetic field.A series of experiments are described here that measure the shape of these

46 CFR 108.641 - Instructions for changing steering gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Instructions for changing steering gear. 108.641 Section... steering gear. Instructions stating, in order, the different steps to be taken for changing to emergency and secondary steering gear must be posted in the steering gear room and at each secondary steering...

α-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate the Initiation of Dynein-Driven Transport in Neurons

Directory of Open Access Journals (Sweden)

Jeffrey J. Nirschl

2016-03-01

Full Text Available Motor-cargo recruitment to microtubules is often the rate-limiting step of intracellular transport, and defects in this recruitment can cause neurodegenerative disease. Here, we use in vitro reconstitution assays with single-molecule resolution, live-cell transport assays in primary neurons, computational image analysis, and computer simulations to investigate the factors regulating retrograde transport initiation in the distal axon. We find that phosphorylation of the cytoskeletal-organelle linker protein CLIP-170 and post-translational modifications of the microtubule track combine to precisely control the initiation of retrograde transport. Computer simulations of organelle dynamics in the distal axon indicate that while CLIP-170 primarily regulates the time to microtubule encounter, the tyrosination state of the microtubule lattice regulates the likelihood of binding. These mechanisms interact to control transport initiation in the axon in a manner sensitive to the specialized cytoskeletal architecture of the neuron.

Analysis of Vehicle Steering and Driving Bifurcation Characteristics

Directory of Open Access Journals (Sweden)

Xianbin Wang

2015-01-01

Full Text Available The typical method of vehicle steering bifurcation analysis is based on the nonlinear autonomous vehicle model deriving from the classic two degrees of freedom (2DOF linear vehicle model. This method usually neglects the driving effect on steering bifurcation characteristics. However, in the steering and driving combined conditions, the tyre under different driving conditions can provide different lateral force. The steering bifurcation mechanism without the driving effect is not able to fully reveal the vehicle steering and driving bifurcation characteristics. Aiming at the aforementioned problem, this paper analyzed the vehicle steering and driving bifurcation characteristics with the consideration of driving effect. Based on the 5DOF vehicle system dynamics model with the consideration of driving effect, the 7DOF autonomous system model was established. The vehicle steering and driving bifurcation dynamic characteristics were analyzed with different driving mode and driving torque. Taking the front-wheel-drive system as an example, the dynamic evolution process of steering and driving bifurcation was analyzed by phase space, system state variables, power spectral density, and Lyapunov index. The numerical recognition results of chaos were also provided. The research results show that the driving mode and driving torque have the obvious effect on steering and driving bifurcation characteristics.

Method of Controlling Steering of a Ground Vehicle

Science.gov (United States)

Dawson, Andrew D. (Inventor); Bluethmann, William J. (Inventor); Lee, Chunhao J. (Inventor); Vitale, Robert L. (Inventor); Guo, Raymond (Inventor); Atluri, Venkata Prasad (Inventor)

2016-01-01

A method of controlling steering of a vehicle through setting wheel angles of a plurality of modular electronic corner assemblies (eModules) is provided. The method includes receiving a driving mode selected from a mode selection menu. A position of a steering input device is determined in a master controller. A velocity of the vehicle is determined, in the master controller, when the determined position of the steering input device is near center. A drive mode request corresponding to the selected driving mode to the plurality of steering controllers is transmitted to the master controller. A required steering angle of each of the plurality of eModules is determined, in the master controller, as a function of the determined position of the steering input device, the determined velocity of the vehicle, and the selected first driving mode. The eModules are set to the respective determined steering angles.

Understanding X-ray cargo imaging

International Nuclear Information System (INIS)

Chen Gongyin

2005-01-01

Cargo imaging is the field of imaging large objects, usually cargo containers, trains, trucks or boats. Transmission imaging with photons, especially X-rays of up to 9 MV is the dominant current technique, providing compelling details of the contents of objects this large. This paper discusses the physics aspects of a good X-ray cargo imaging system. The basic performance requirements, such as penetration, contrast and resolution and the components of a cargo imaging system are introduced. The imaging process is divided in this paper into three stages: forming information (probing the object), recording information and presenting information (image display). Their impact on performance is analyzed

46 CFR 61.20-1 - Steering gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Steering gear. 61.20-1 Section 61.20-1 Shipping COAST... Periodic Tests of Machinery and Equipment § 61.20-1 Steering gear. (a) The marine inspector must inspect the steering gear at each inspection for certification for vessels whose Certificate of Inspections...

Characterizing X-ray Attenuation of Containerized Cargo

Energy Technology Data Exchange (ETDEWEB)

Birrer, N. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Divin, C. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Glenn, S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Martz, H. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Wang, G. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2016-08-02

X-ray inspection systems can be used to detect radiological and nuclear threats in imported cargo. In order to better understand performance of these systems, the attenuation characteristics of imported cargo need to be determined. This project focused on developing image processing algorithms for segmenting cargo and using x-ray attenuation to quantify equivalent steel thickness to determine cargo density. These algorithms were applied to over 450 cargo radiographs. The results are summarized in this report.

Relative entropy of steering: on its definition and properties

International Nuclear Information System (INIS)

Kaur, Eneet; Wilde, Mark M

2017-01-01

In Gallego and Aolita (2015 Phys. Rev . X 5 041008), the authors proposed a definition for the relative entropy of steering and showed that the resulting quantity is a convex steering monotone. Here we advocate for a different definition for relative entropy of steering, based on well grounded concerns coming from quantum Shannon theory. We prove that this modified relative entropy of steering is a convex steering monotone. Furthermore, we establish that it is uniformly continuous and faithful, in both cases giving quantitative bounds that should be useful in applications. We also consider a restricted relative entropy of steering which is relevant for the case in which the free operations in the resource theory of steering have a more restricted form (the restricted operations could be more relevant in practical scenarios). The restricted relative entropy of steering is convex, monotone with respect to these restricted operations, uniformly continuous, and faithful. (paper)

Wireless traffic steering for green cellular networks

CERN Document Server

Zhang, Shan; Zhou, Sheng; Niu, Zhisheng; Shen, Xuemin (Sherman)

2016-01-01

This book introduces wireless traffic steering as a paradigm to realize green communication in multi-tier heterogeneous cellular networks. By matching network resources and dynamic mobile traffic demand, traffic steering helps to reduce on-grid power consumption with on-demand services provided. This book reviews existing solutions from the perspectives of energy consumption reduction and renewable energy harvesting. Specifically, it explains how traffic steering can improve energy efficiency through intelligent traffic-resource matching. Several promising traffic steering approaches for dynamic network planning and renewable energy demand-supply balancing are discussed. This book presents an energy-aware traffic steering method for networks with energy harvesting, which optimizes the traffic allocated to each cell based on the renewable energy status. Renewable energy demand-supply balancing is a key factor in energy dynamics, aimed at enhancing renewable energy sustainability to reduce on-grid energy consum...

The lissencephaly protein Lis1 is present in motile mammalian cilia and requires outer arm dynein for targeting to Chlamydomonas flagella

DEFF Research Database (Denmark)

Pedersen, Lotte B; Rompolas, Panteleimon; Christensen, Søren T

2007-01-01

Lissencephaly is a developmental brain disorder characterized by a smooth cerebral surface, thickened cortex and misplaced neurons. Classical lissencephaly is caused by mutations in LIS1, which encodes a WD-repeat protein involved in cytoplasmic dynein regulation, mitosis and nuclear migration. S...

46 CFR 98.30-11 - Cargo pumps.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo pumps. 98.30-11 Section 98.30-11 Shipping COAST..., ARRANGEMENT, AND OTHER PROVISIONS FOR CERTAIN DANGEROUS CARGOES IN BULK Portable Tanks § 98.30-11 Cargo pumps. No person may operate a cargo pump to transfer a product to or from a portable tank unless the pump...

South Africa’s freight rail reform: A demand-driven perspective

Directory of Open Access Journals (Sweden)

Jan H. Havenga

2014-11-01

Full Text Available During the 20th century, freight transport in South Africa was employed to attain politicoeconomic ideals, resulting in the overprotection of rail and overregulation of road transport. Increasing industry pressure, combined with the international deregulation trend, led to deregulation in 1988. Myopia resulted in a rail investment hiatus and exponential growth in high-value, long-distance road transport, causing excessive logistics and externality costs for the country. The aim of this study was to propose a freight rail reform agenda based on, (1 lessons from past freight transport policy efforts and (2 the results of freight transport market segmentation driven by models developed over the past two decades. For the study, freight flows were modelled by disaggregating the national input–output model into 372 origin–destination pairs and 71 commodity groups, followed by distance decay gravitymodelling. Logistics costs were calculated by relating commodity-level freight flows to the costs of fulfilling associated logistical functions. The standard management approach of founding strategy development on market-driven segmentation provides a neutral input to steer rail reform discussions in South Africa. Market segmentation points to a dualistic rail reform agenda, enabling both a profit-driven core and a development-driven branch line network. Freight flow insights are steering the policy reform debate towards long-term freight strategy development and optimal freight logistics network design.

Product development of Indian cargo scanner

International Nuclear Information System (INIS)

2017-01-01

A cargo scanner is required for nonintrusive screening of suspected cargo containers in trade, using high energy X-ray, to detect any mis-declarations, contraband goods concealment or hidden ammunition or explosives. The cargo scanners help authorities to process large number of suspected cargo with a high level of confidence with other additional benefit of faster clearance, minimised intrusive inspection and generating secured digital record of the process. BARC is in process of developing Indian Cargo Scanner with indigenous X-ray source. Proof of concept and conformance of the results to the international standards has been demonstrated in laboratory. Full scale equipment named as Portal scanner shall be demonstrated at Gamma field Trombay in year 2017. Subsequently the technology transfer may be done to a suitable Indian vendor

Automated beam steering using optimal control

Energy Technology Data Exchange (ETDEWEB)

Allen, C. K. (Christopher K.)

2004-01-01

We present a steering algorithm which, with the aid of a model, allows the user to specify beam behavior throughout a beamline, rather than just at specified beam position monitor (BPM) locations. The model is used primarily to compute the values of the beam phase vectors from BPM measurements, and to define cost functions that describe the steering objectives. The steering problem is formulated as constrained optimization problem; however, by applying optimal control theory we can reduce it to an unconstrained optimization whose dimension is the number of control signals.

Acoustic Multipurpose Cargo Transfer Bag

Science.gov (United States)

Baccus, Shelley

2015-01-01

The Logistics Reduction (LR) project within the Advanced Exploration Systems (AES) program is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) are designed to be the same external volume as a regular cargo transfer bag, the common logistics carrier for the International Space Station. After use as a cargo bag, the MCTB can be unzipped and unfolded to be reused. This Acoustic MCTBs transform into acoustic blankets after the initial logistics carrying objective is complete.

Speed choice and steering behavior in curve driving

NARCIS (Netherlands)

Winsum, W. van; Godthelp, J.

1996-01-01

The relation between speed choice and steering performance during curve negotiation was studied in a driving simulator. The hypothesis was that curve radius and steering competence both affect steering error during curve driving, resulting in compensatory speed choice. In this, the control of safety

46 CFR 58.25-20 - Piping for steering gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Piping for steering gear. 58.25-20 Section 58.25-20... MACHINERY AND RELATED SYSTEMS Steering Gear § 58.25-20 Piping for steering gear. (a) Pressure piping must... the hydraulic system can be readily recharged from within the steering-gear compartment and must be...

Characterizing Complexity of Containerized Cargo X-ray Images

Energy Technology Data Exchange (ETDEWEB)

Wang, Guangxing [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Martz, Harry [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Glenn, Steven [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Divin, Charles [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Birrer, Nat [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2016-08-19

X-ray imaging can be used to inspect cargos imported into the United States. In order to better understand the performance of X-ray inspection systems, the X-ray characteristics (density, complexity) of cargo need to be quantified. In this project, an image complexity measure called integrated power spectral density (IPSD) was studied using both DNDO engineered cargos and stream-of-commerce (SOC) cargos. A joint distribution of cargo density and complexity was obtained. A support vector machine was used to classify the SOC cargos into four categories to estimate the relative fractions.

Characterizing Complexity of Containerized Cargo X-ray Images

International Nuclear Information System (INIS)

Wang, Guangxing; Martz, Harry; Glenn, Steven; Divin, Charles; Birrer, Nat

2016-01-01

X-ray imaging can be used to inspect cargos imported into the United States. In order to better understand the performance of X-ray inspection systems, the X-ray characteristics (density, complexity) of cargo need to be quantified. In this project, an image complexity measure called integrated power spectral density (IPSD) was studied using both DNDO engineered cargos and stream-of-commerce (SOC) cargos. A joint distribution of cargo density and complexity was obtained. A support vector machine was used to classify the SOC cargos into four categories to estimate the relative fractions.

Improving Einstein–Podolsky–Rosen steering inequalities with state information

International Nuclear Information System (INIS)

Schneeloch, James; Broadbent, Curtis J.; Howell, John C.

2014-01-01

We discuss the relationship between entropic Einstein–Podolsky–Rosen (EPR)-steering inequalities and their underlying uncertainty relations along with the hypothesis that improved uncertainty relations lead to tighter EPR-steering inequalities. In particular, we discuss how using information about the state of a quantum system affects one's ability to witness EPR-steering. As an example, we consider the recent improvement to the entropic uncertainty relation between pairs of discrete observables (Berta et al., 2010 [10]). By considering the assumptions that enter into the development of a steering inequality, we derive correct steering inequalities from these improved uncertainty relations and find that they are identical to ones already developed (Schneeloch et al., 2013 [9]). In addition, we consider how one can use state information to improve our ability to witness EPR-steering, and develop a new continuous variable symmetric EPR-steering inequality as a result.

Driver assistance system for passive multi-trailer vehicles with haptic steering limitations on the leading unit.

Science.gov (United States)

Morales, Jesús; Mandow, Anthony; Martínez, Jorge L; Reina, Antonio J; García-Cerezo, Alfonso

2013-04-03

Driving vehicles with one or more passive trailers has difficulties in both forward and backward motion due to inter-unit collisions, jackknife, and lack of visibility. Consequently, advanced driver assistance systems (ADAS) for multi-trailer combinations can be beneficial to accident avoidance as well as to driver comfort. The ADAS proposed in this paper aims to prevent unsafe steering commands by means of a haptic handwheel. Furthermore, when driving in reverse, the steering-wheel and pedals can be used as if the vehicle was driven from the back of the last trailer with visual aid from a rear-view camera. This solution, which can be implemented in drive-by-wire vehicles with hitch angle sensors, profits from two methods previously developed by the authors: safe steering by applying a curvature limitation to the leading unit, and a virtual tractor concept for backward motion that includes the complex case of set-point propagation through on-axle hitches. The paper addresses system requirements and provides implementation details to tele-operate two different off- and on-axle combinations of a tracked mobile robot pulling and pushing two dissimilar trailers.

Driver Assistance System for Passive Multi-Trailer Vehicles with Haptic Steering Limitations on the Leading Unit

Directory of Open Access Journals (Sweden)

Antonio J. Reina

2013-04-01

Full Text Available Driving vehicles with one or more passive trailers has difficulties in both forward and backward motion due to inter-unit collisions, jackknife, and lack of visibility. Consequently, advanced driver assistance systems (ADAS for multi-trailer combinations can be beneficial to accident avoidance as well as to driver comfort. The ADAS proposed in this paper aims to prevent unsafe steering commands by means of a haptic handwheel. Furthermore, when driving in reverse, the steering-wheel and pedals can be used as if the vehicle was driven from the back of the last trailer with visual aid from a rear-view camera. This solution, which can be implemented in drive-by-wire vehicles with hitch angle sensors, profits from two methods previously developed by the authors: safe steering by applying a curvature limitation to the leading unit, and a virtual tractor concept for backward motion that includes the complex case of set-point propagation through on-axle hitches. The paper addresses system requirements and provides implementation details to tele-operate two different off- and on-axle combinations of a tracked mobile robot pulling and pushing two dissimilar trailers.

Assessing the Impact of Electrostatic Drag on Processive Molecular Motor Transport.

Science.gov (United States)

Smith, J Darby; McKinley, Scott A

2018-06-04

The bidirectional movement of intracellular cargo is usually described as a tug-of-war among opposite-directed families of molecular motors. While tug-of-war models have enjoyed some success, recent evidence suggests underlying motor interactions are more complex than previously understood. For example, these tug-of-war models fail to predict the counterintuitive phenomenon that inhibiting one family of motors can decrease the functionality of opposite-directed transport. In this paper, we use a stochastic differential equations modeling framework to explore one proposed physical mechanism, called microtubule tethering, that could play a role in this "co-dependence" among antagonistic motors. This hypothesis includes the possibility of a trade-off: weakly bound trailing molecular motors can serve as tethers for cargoes and processing motors, thereby enhancing motor-cargo run lengths along microtubules; however, this introduces a cost of processing at a lower mean velocity. By computing the small- and large-time mean-squared displacement of our theoretical model and comparing our results to experimental observations of dynein and its "helper protein" dynactin, we find some supporting evidence for microtubule tethering interactions. We extrapolate these findings to predict how dynein-dynactin might interact with the opposite-directed kinesin motors and introduce a criterion for when the trade-off is beneficial in simple systems.

Fast track, dynein-dependent nuclear targeting of human immunodeficiency virus Vpr protein; impaired trafficking in a clinical isolate

Energy Technology Data Exchange (ETDEWEB)

Caly, Leon [Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800 (Australia); Kassouf, Vicki T. [Centre for Virus Research, The Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW 2145 (Australia); Moseley, Gregory W. [Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800 (Australia); Diefenbach, Russell J.; Cunningham, Anthony L. [Centre for Virus Research, The Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW 2145 (Australia); Jans, David A., E-mail: david.jans@monash.edu [Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800 (Australia)

2016-02-12

Nuclear import of the accessory protein Vpr is central to infection by human immunodeficiency virus (HIV). We previously identified the Vpr F72L mutation in a HIV-infected, long-term non-progressor, showing that it resulted in reduced Vpr nuclear accumulation and altered cytoplasmic localisation. Here we demonstrate for the first time that the effects of nuclear accumulation of the F72L mutation are due to impairment of microtubule-dependent-enhancement of Vpr nuclear import. We use high resolution imaging approaches including fluorescence recovery after photobleaching and other approaches to document interaction between Vpr and the dynein light chain protein, DYNLT1, and impaired interaction of the F72L mutant with DYNLT1. The results implicate MTs/DYNLT1 as drivers of Vpr nuclear import and HIV infection, with important therapeutic implications. - Highlights: • HIV-1 Vpr utilizes the microtubule network to traffic towards the nucleus. • Mechanism relies on interaction between Vpr and dynein light chain protein DYNLT1. • Long-term non-progressor derived mutation (F72L) impairs this interaction. • Key residues in the vicinity of F72 contribute to interaction with DYNLT1.

Fast track, dynein-dependent nuclear targeting of human immunodeficiency virus Vpr protein; impaired trafficking in a clinical isolate

International Nuclear Information System (INIS)

Caly, Leon; Kassouf, Vicki T.; Moseley, Gregory W.; Diefenbach, Russell J.; Cunningham, Anthony L.; Jans, David A.

2016-01-01

Nuclear import of the accessory protein Vpr is central to infection by human immunodeficiency virus (HIV). We previously identified the Vpr F72L mutation in a HIV-infected, long-term non-progressor, showing that it resulted in reduced Vpr nuclear accumulation and altered cytoplasmic localisation. Here we demonstrate for the first time that the effects of nuclear accumulation of the F72L mutation are due to impairment of microtubule-dependent-enhancement of Vpr nuclear import. We use high resolution imaging approaches including fluorescence recovery after photobleaching and other approaches to document interaction between Vpr and the dynein light chain protein, DYNLT1, and impaired interaction of the F72L mutant with DYNLT1. The results implicate MTs/DYNLT1 as drivers of Vpr nuclear import and HIV infection, with important therapeutic implications. - Highlights: • HIV-1 Vpr utilizes the microtubule network to traffic towards the nucleus. • Mechanism relies on interaction between Vpr and dynein light chain protein DYNLT1. • Long-term non-progressor derived mutation (F72L) impairs this interaction. • Key residues in the vicinity of F72 contribute to interaction with DYNLT1.

Cargo Data Management Demonstration System

Science.gov (United States)

1974-02-01

Delays in receipt and creation of cargo documents are a problem in international trade. The work described demonstrates some of the advantages and capabilities of a computer-based cargo data management system. A demonstration system for data manageme...

Steer-by-wire innovations and demonstrator

NARCIS (Netherlands)

Lupker, H.A.; Zuurbier, J.; Verschuren, R.M.A.F.; Jansen, S.T.H.; Willemsen, D.M.C.

2002-01-01

Arguments for 'by-wire' systems include production costs, packaging and traffic safety. Innovations concern both product and development process e.g. combined virtual engineering and Hardware-in-the-loop testing. Three Steer-by-wire systems are discussed: a steering system simulator used as a

Design and manufacturing of mechanical steering system for ...

African Journals Online (AJOL)

Design and manufacturing of mechanical steering system for parallel parking, zero turning radius, minimum turning radius with traditional turning. ... of the steering system are designed so as to meet all the configuration of steering system and to be well-matched to the power train, suspension system and body of the car.

Research on Performance of Wire-controlled Hydraulic Steering System Based on Four-wheel Steering

Science.gov (United States)

Tao, P.; Jin, X. H.

2018-05-01

In this paper, the steering stability and control strategy of forklift are put forward. Drive based on yawing moment distribution of rotary torque coordination control method, through analyzing the linear two degree of freedom model of forklift truck, forklift yawing angular velocity and mass center side-slip Angle of expectations, as the control target parameters system, using fuzzy controller output driving forklift steering the yawing moment, to drive rotary torque distribution, make the forklift truck to drive horizontal pendulum angular velocity and side-slip Angle tracking reference model very well. In this paper, the lateral stability control system were designed, the joint simulation in MATLAB/Simulink, the simulation results show that under the different partial load, the control system can effectively to control side forklift lateral stability, enhanced the forklift driving safety, for the side forklift steering stability study provides a theoretical basis.

Liberalisation of air cargo transport

Science.gov (United States)

2002-05-02

Over a period of many years, international air cargo demand has continued to increase more rapidly than international air passenger demand. Air cargo arrangements need to be as efficient and expeditious as possible, to meet user requirements for air ...

46 CFR 167.65-25 - Steering gear tests.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Steering gear tests. 167.65-25 Section 167.65-25... SHIPS Special Operating Requirements § 167.65-25 Steering gear tests. On all nautical school ships making voyages of more than 48 hours' duration, the entire steering gear, the whistle, the means of...

Salmonella Disrupts Host Endocytic Trafficking by SopD2-Mediated Inhibition of Rab7

Directory of Open Access Journals (Sweden)

Vanessa M. D’Costa

2015-09-01

Full Text Available Intracellular bacterial pathogens of a diverse nature share the ability to evade host immunity by impairing trafficking of endocytic cargo to lysosomes for degradation, a process that is poorly understood. Here, we show that the Salmonella enterica type 3 secreted effector SopD2 mediates this process by binding the host regulatory GTPase Rab7 and inhibiting its nucleotide exchange. Consequently, this limits Rab7 interaction with its dynein- and kinesin-binding effectors RILP and FYCO1 and thereby disrupts host-driven regulation of microtubule motors. Our study identifies a bacterial effector capable of directly binding and thereby modulating Rab7 activity and a mechanism of endocytic trafficking disruption that may provide insight into the pathogenesis of other bacteria. Additionally, we provide a powerful tool for the study of Rab7 function, and a potential therapeutic target.

Quantum Steering Beyond Instrumental Causal Networks

Science.gov (United States)

Nery, R. V.; Taddei, M. M.; Chaves, R.; Aolita, L.

2018-04-01

We theoretically predict, and experimentally verify with entangled photons, that outcome communication is not enough for hidden-state models to reproduce quantum steering. Hidden-state models with outcome communication correspond, in turn, to the well-known instrumental processes of causal inference but in the one-sided device-independent scenario of one black-box measurement device and one well-characterized quantum apparatus. We introduce one-sided device-independent instrumental inequalities to test against these models, with the appealing feature of detecting entanglement even when communication of the black box's measurement outcome is allowed. We find that, remarkably, these inequalities can also be violated solely with steering, i.e., without outcome communication. In fact, an efficiently computable formal quantifier—the robustness of noninstrumentality—naturally arises, and we prove that steering alone is enough to maximize it. Our findings imply that quantum theory admits a stronger form of steering than known until now, with fundamental as well as practical potential implications.

46 CFR 154.562 - Cargo hose: Hydrostatic test.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo hose: Hydrostatic test. 154.562 Section 154.562 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY... Hose § 154.562 Cargo hose: Hydrostatic test. Each cargo hose must pass a hydrostatic pressure test at...

Assessment of a Boat Fractured Steering Wheel

Directory of Open Access Journals (Sweden)

Vukelic Goran

2016-09-01

Full Text Available During regular use of the steering wheel mounted on a boat, two cracks emanating from a fastener hole were noticed which, consequently, caused final fracture of the wheel. To determine the behavior of a boat steering wheel with cracks present, assessment of a fractured wheel was performed. Torque moments of the fasteners were measured prior to removing the steering wheel from the boat. Visual and dye penetrant inspection followed along with the material detection. Besides using experimental procedures, assessment of the fractured wheel was performed using finite element analysis, i.e. stress intensity factor values were numerically determined. Variation of stress intensity factor with crack length is presented. Possible causes of crack occurrence are given and they include excessive values of fastener torque moments coupled with fretting between fastener and fastener hole that was poorly machined. Results obtained by this assessment can be taken for predicting fracture behavior of a cracked steering wheel and as a reference in the design, mounting and exploitation process of steering wheels improving that way their safety in transportation environment.

Safety of Cargo Aircraft Handling Procedure

Directory of Open Access Journals (Sweden)

Daniel Hlavatý

2017-07-01

Full Text Available The aim of this paper is to get acquainted with the ways how to improve the safety management system during cargo aircraft handling. The first chapter is dedicated to general information about air cargo transportation. This includes the history or types of cargo aircraft handling, but also the means of handling. The second part is focused on detailed description of cargo aircraft handling, including a description of activities that are performed before and after handling. The following part of this paper covers a theoretical interpretation of safety, safety indicators and legislative provisions related to the safety of cargo aircraft handling. The fourth part of this paper analyzes the fault trees of events which might occur during handling. The factors found by this analysis are compared with safety reports of FedEx. Based on the comparison, there is a proposal on how to improve the safety management in this transportation company.

Holographic memory using beam steering

Science.gov (United States)

Chao, Tien-Hsin (Inventor); Hanan, Jay C. (Inventor); Reyes, George F. (Inventor); Zhou, Hanying (Inventor)

2006-01-01

A method, apparatus, and system provide the ability for storing holograms at high speed. A single laser diode emits a collimated laser beam to both write to and read from a photorefractice crystal. One or more liquid crystal beam steering spatial light modulators (BSSLMs) or Micro-Electro-Mechanical Systems (MEMS) mirrors steer a reference beam, split from the collimated laser beam, at high speed to the photorefractive crystal.

Steering Angle Function Algorithm of Morphing of Residential Area

Directory of Open Access Journals (Sweden)

XIE Tian

2015-07-01

Full Text Available A residential area feature morphing method based on steering angle function is presented. To residential area with the same representation under two different scales,transforming the representation of the residential area polygon from vector coordinates to steering angle function,then using the steering angle function to match,and finding out the similarity and the differences between the residential areas under different scale to get the steering angle function of the the residential areas under any middle scale,the final,transforming the middle scale steering angle function to vector coordinates form,and get the middle shape interpolation of the the residential area polygon.Experimental results show:the residential area morphing method by using steering angle function presented can realize the continuous multi-scale representation under the premise of keeping in shape for the residential area with the rectangular boundary features.

Schwann cell myelination requires Dynein function

Directory of Open Access Journals (Sweden)

Langworthy Melissa M

2012-11-01

Full Text Available Abstract Background Interaction of Schwann cells with axons triggers signal transduction that drives expression of Pou3f1 and Egr2 transcription factors, which in turn promote myelination. Signal transduction appears to be mediated, at least in part, by cyclic adenosine monophosphate (cAMP because elevation of cAMP levels can stimulate myelination in the absence of axon contact. The mechanisms by which the myelinating signal is conveyed remain unclear. Results By analyzing mutations that disrupt myelination in zebrafish, we learned that Dynein cytoplasmic 1 heavy chain 1 (Dync1h1, which functions as a motor for intracellular molecular trafficking, is required for peripheral myelination. In dync1h1 mutants, Schwann cell progenitors migrated to peripheral nerves but then failed to express Pou3f1 and Egr2 or make myelin membrane. Genetic mosaic experiments revealed that robust Myelin Basic Protein expression required Dync1h1 function within both Schwann cells and axons. Finally, treatment of dync1h1 mutants with a drug to elevate cAMP levels stimulated myelin gene expression. Conclusion Dync1h1 is required for retrograde transport in axons and mutations of Dync1h1 have been implicated in axon disease. Our data now provide evidence that Dync1h1 is also required for efficient myelination of peripheral axons by Schwann cells, perhaps by facilitating signal transduction necessary for myelination.

46 CFR 153.440 - Cargo temperature sensors.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo temperature sensors. 153.440 Section 153.440 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS... Temperature Control Systems § 153.440 Cargo temperature sensors. (a) Except as prescribed in paragraph (c) of...

46 CFR 196.37-33 - Instructions for changing steering gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Instructions for changing steering gear. 196.37-33... steering gear. (a) Instructions in at least 1/2 inch letters and figures shall be posted in the steering engineroom, relating in order, the different steps to be taken in changing to the emergency steering gear...

46 CFR 58.25-10 - Main and auxiliary steering gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Main and auxiliary steering gear. 58.25-10 Section 58.25... AUXILIARY MACHINERY AND RELATED SYSTEMS Steering Gear § 58.25-10 Main and auxiliary steering gear. (a) Power-operated main and auxiliary steering gear must be separate systems that are independent throughout their...

Steering Your Mysterious Mind

DEFF Research Database (Denmark)

Prasad, Ramjee

Steering the Mysterious Mind, describes a unique, novel concept for a way to gain control of your mind. The five basic elements of human life, that is; Creativity, Content­ment, Confidence, Calmness, and Concentration (C5) have been introduced in my previous book Unlock Your Personalization. Posi....... Compare it with going to the gym where you work on the physical body. In the same way as with arms and legs, the mind is a mus­cle which you exercise through C5 practice. Steering the mind on your personal goal will help you to be creative....

Working paper on public steering of privately owned sports facilities

DEFF Research Database (Denmark)

Iversen, Evald Bundgård

This short paper discusses how municipalities can steer privately owned sports facilities. Firstly I analyse why steering of privately owned facilities is an interesting subject. Secondly I discuss what the advantages and drawbacks of using different approaches for steering sports facilities are........ Finally I discuss the methodological challenges of measuring activities in sports facilities – and take a closer look at the advantages and drawbacks of using manual and thermal techniques for registering activity.......This short paper discusses how municipalities can steer privately owned sports facilities. Firstly I analyse why steering of privately owned facilities is an interesting subject. Secondly I discuss what the advantages and drawbacks of using different approaches for steering sports facilities are...

46 CFR 154.1816 - Cargo location plan.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo location plan. 154.1816 Section 154.1816 Shipping... FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES Operations § 154.1816 Cargo location plan. The master shall ensure that: (a) A cargo location plan is prepared that gives: (1) The location and...

29 CFR 1918.87 - Ship's cargo elevators.

Science.gov (United States)

2010-07-01

... 29 Labor 7 2010-07-01 2010-07-01 false Ship's cargo elevators. 1918.87 Section 1918.87 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR LONGSHORING Handling Cargo § 1918.87 Ship's cargo elevators. (a) Safe working load. The safe workin...

Specialization and Flexibility in Port Cargo Handling

Directory of Open Access Journals (Sweden)

Hakkı KİŞİ

2016-11-01

Full Text Available Cargo handling appears to be the fundamental function of ports. In this context, the question of type of equipment and capacity rate need to be tackled with respect to cargo handling principles. The purpose of this study is to discuss the types of equipment to be used in ports, relating the matter to costs and capacity. The question is studied with a basic economic theoretical approach. Various conditions like port location, size, resources, cargo traffic, ships, etc. are given parameters to dictate the type and specification of the cargo handling equipment. Besides, a simple approach in the context of cost capacity relation can be useful in deciding whether to use specialized or flexible equipment. Port equipment is sometimes expected to be flexible to handle various types of cargo as many as possible and sometimes to be specialized to handle one specific type of cargo. The cases that might be suitable for those alternatives are discussed from an economic point of view in this article. Consequently, effectiveness and efficiency criteria play important roles in determining the handling equipment in ports.

46 CFR 154.1814 - Cargo information cards.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo information cards. 154.1814 Section 154.1814... cards. (a) No person may operate a vessel unless a cargo information card for each cargo being... accessible to the person in charge of the watch. (b) When a vessel is moored at a terminal, the master shall...

Cargo Release from Polymeric Vesicles under Shear

Directory of Open Access Journals (Sweden)

Yingying Guo

2018-03-01

Full Text Available In this paper we study the release of cargo from polymeric nano-carriers under shear. Vesicles formed by two star block polymers— A 12 B 6 C 2 ( A B C and A 12 B 6 A 2 ( A B A —and one linear block copolymer— A 14 B 6 ( A B , are investigated using dissipative particle dynamics (DPD simulations. A - and C -blocks are solvophobic and B -block is solvophilic. The three polymers form vesicles of different structures. The vesicles are subjected to shear both in bulk and between solvophobic walls. In bulk shear, the mechanisms of cargo release are similar for all vesicles, with cargo travelling through vesicle membrane with no preferential release location. When sheared between walls, high cargo release rate is only observed with A B C vesicle after it touches the wall. For A B C vesicle, the critical condition for high cargo release rate is the formation of wall-polymersome interface after which the effect of shear rate in promoting cargo release is secondary. High release rate is achieved by the formation of solvophilic pathway allowing cargo to travel from the vesicle cavity to the vesicle exterior. The results in this paper show that well controlled target cargo release using polymersomes can be achieved with polymers of suitable design and can potentially be very useful for engineering applications. As an example, polymersomes can be used as carriers for surface active friction reducing additives which are only released at rubbing surfaces where the additives are needed most.

Observation of one-way Einstein-Podolsky-Rosen steering

Science.gov (United States)

Händchen, Vitus; Eberle, Tobias; Steinlechner, Sebastian; Samblowski, Aiko; Franz, Torsten; Werner, Reinhard F.; Schnabel, Roman

2012-09-01

The distinctive non-classical features of quantum physics were first discussed in the seminal paper by A. Einstein, B. Podolsky and N. Rosen (EPR) in 1935. In his immediate response, E. Schrödinger introduced the notion of entanglement, now seen as the essential resource in quantum information as well as in quantum metrology. Furthermore, he showed that at the core of the EPR argument is a phenomenon that he called steering. In contrast to entanglement and violations of Bell's inequalities, steering implies a direction between the parties involved. Recent theoretical works have precisely defined this property, but the question arose as to whether there are bipartite states showing steering only in one direction. Here, we present an experimental realization of two entangled Gaussian modes of light that in fact shows the steering effect in one direction but not in the other. The generated one-way steering gives a new insight into quantum physics and may open a new field of applications in quantum information.

Refractive waveguide non-mechanical beam steering (NMBS) in the MWIR

Science.gov (United States)

Myers, Jason D.; Frantz, Jesse A.; Spillmann, Christopher M.; Bekele, Robel Y.; Kolacz, Jakub; Gotjen, Henry; Naciri, Jawad; Shaw, Brandon; Sanghera, Jas S.

2018-02-01

Beam steering is a crucial technology for a number of applications, including chemical sensing/mapping and light detection and ranging (LIDAR). Traditional beam steering approaches rely on mechanical movement, such as the realignment of mirrors in gimbal mounts. The mechanical approach to steering has several drawbacks, including large size, weight and power usage (SWAP), and frequent mechanical failures. Recently, alternative non-mechanical approaches have been proposed and developed, but these technologies do not meet the demanding requirements for many beam steering applications. Here, we highlight the development efforts into a particular non-mechanical beam steering (NMBS) approach, refractive waveguides, for application in the MWIR. These waveguides are based on an Ulrich-coupled slab waveguide with a liquid crystal (LC) top cladding; by selectively applying an electric field across the liquid crystal through a prismatic electrode, steering is achieved by creating refraction at prismatic interfaces as light propagates through the device. For applications in the MWIR, we describe a versatile waveguide architecture based on chalcogenide glasses that have a wide range of refractive indices, transmission windows, and dispersion properties. We have further developed robust shadow-masking methods to taper the subcladding layers in the coupling region. We have demonstrated devices with >10° of steering in the MWIR and a number of advantageous properties for beam steering applications, including low-power operation, compact size, and fast point-to-point steering.

Optimization Under Uncertainty for Wake Steering Strategies: Preprint

Energy Technology Data Exchange (ETDEWEB)

Quick, Julian [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Annoni, Jennifer [National Renewable Energy Laboratory (NREL), Golden, CO (United States); King, Ryan N [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Dykes, Katherine L [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Fleming, Paul A [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Ning, Andrew [Brigham Young University

2017-05-01

Wind turbines in a wind power plant experience significant power losses because of aerodynamic interactions between turbines. One control strategy to reduce these losses is known as 'wake steering,' in which upstream turbines are yawed to direct wakes away from downstream turbines. Previous wake steering research has assumed perfect information, however, there can be significant uncertainty in many aspects of the problem, including wind inflow and various turbine measurements. Uncertainty has significant implications for performance of wake steering strategies. Consequently, the authors formulate and solve an optimization under uncertainty (OUU) problem for finding optimal wake steering strategies in the presence of yaw angle uncertainty. The OUU wake steering strategy is demonstrated on a two-turbine test case and on the utility-scale, offshore Princess Amalia Wind Farm. When we accounted for yaw angle uncertainty in the Princess Amalia Wind Farm case, inflow-direction-specific OUU solutions produced between 0% and 1.4% more power than the deterministically optimized steering strategies, resulting in an overall annual average improvement of 0.2%. More importantly, the deterministic optimization is expected to perform worse and with more downside risk than the OUU result when realistic uncertainty is taken into account. Additionally, the OUU solution produces fewer extreme yaw situations than the deterministic solution.

BEAMLINE-CONTROLLED STEERING OF SOURCE-POINT ANGLE AT THE ADVANCED PHOTON SOURCE

Energy Technology Data Exchange (ETDEWEB)

Emery, L.; Fystro, G.; Shang, H.; Smith, M.

2017-06-25

An EPICS-based steering software system has been implemented for beamline personnel to directly steer the angle of the synchrotron radiation sources at the Advanced Photon Source. A script running on a workstation monitors "start steering" beamline EPICS records, and effects a steering given by the value of the "angle request" EPICS record. The new system makes the steering process much faster than before, although the older steering protocols can still be used. The robustness features of the original steering remain. Feedback messages are provided to the beamlines and the accelerator operators. Underpinning this new steering protocol is the recent refinement of the global orbit feedback process whereby feedforward of dipole corrector set points and orbit set points are used to create a local steering bump in a rapid and seamless way.

Bulling among yearling feedlot steers.

Science.gov (United States)

Pierson, R E; Jensen, R; Braddy, P M; Horton, D P; Christie, R M

1976-09-01

In a survey to determine the cause of illness and deaths among yearling feedlot cattle, bulling was found to be one of the major problems. During the years 1971-1974, 54,913 (2.88%) steers became bullers and represented an annual loss of around +325,000. Some of the causes of bulling were found to be hormones, either as implants or in the feed. In 1974, from 1,988 necropsies, it was determined that 83 steers died from riding injuries.

Fault-tolerant Actuator System for Electrical Steering of Vehicles

DEFF Research Database (Denmark)

Sørensen, Jesper Sandberg; Blanke, Mogens

2006-01-01

is needed that meets this requirement. This paper studies the fault-tolerance properties of an electrical steering system. It presents a fault-tolerant architecture where a dedicated AC motor design used in conjunction with cheap voltage measurements can ensure detection of all relevant faults......Being critical to the safety of vehicles, the steering system is required to maintain the vehicles ability to steer until it is brought to halt, should a fault occur. With electrical steering becoming a cost-effective candidate for electrical powered vehicles, a fault-tolerant architecture...

Methodologies for steering clocks

Science.gov (United States)

Chadsey, Harold

1995-01-01

One of the concerns of the PTTI community is the coordination of one time scale with another. This is accomplished through steering one clock system to another, with a goal of a zero or constant offset in time and frequency. In order to attain this goal, rate differences are calculated and allowed for by the steering algorithm. This paper will present several of these different methods of determining rate differences. Ideally, any change in rate should not cause the offset to change sign (overshoot) by any amount, but certainly not by as much as its previous absolute value. The advantages and disadvantages of each depend on the user's situation.

Collective multipartite Einstein-Podolsky-Rosen steering: more secure optical networks.

Science.gov (United States)

Wang, Meng; Gong, Qihuang; He, Qiongyi

2014-12-01

Collective multipartite Einstein-Podolsky-Rosen (EPR) steering is a type of quantum correlation shared among N parties, where the EPR paradox of one party can only be realized by performing local measurements on all the remaining N-1 parties. We formalize the collective tripartite steering in terms of local hidden state model and give the steering inequalities that act as signatures and suggest how to optimize collective tripartite steering in specific optical schemes. The special entangled states with property of collective multipartite steering may have potential applications in ultra-secure multiuser communication networks where the issue of trust is critical.

Directional control-response compatibility of joystick steered shuttle cars.

Science.gov (United States)

Burgess-Limerick, Robin; Zupanc, Christine M; Wallis, Guy

2012-01-01

Shuttle cars are an unusual class of vehicle operated in underground coal mines, sometimes in close proximity to pedestrians and steering errors may have very serious consequences. A directional control-response incompatibility has previously been described in shuttle cars which are controlled using a steering wheel oriented perpendicular to the direction of travel. Some other shuttle car operators are seated perpendicular to the direction of travel and steer the car via a seat mounted joystick. A virtual simulation was utilised to determine whether the steering arrangement in these vehicles maintains directional control-response compatibility. Twenty-four participants were randomly assigned to either a condition corresponding to this design (consistent direction), or a condition in which the directional steering response was reversed while driving in-bye (visual field compatible). Significantly less accurate steering performance was exhibited by the consistent direction group during the in-bye trials only. Shuttle cars which provide the joystick steering mechanism described here require operators to accommodate alternating compatible and incompatible directional control-response relationships with each change of car direction. A virtual simulation of an underground coal shuttle car demonstrates that the design incorporates a directional control-response incompatibility when driving the vehicle in one direction. This design increases the probability of operator error, with potential adverse safety and productivity consequences.

Research on Handling Stability of Steering-by-wire System

Directory of Open Access Journals (Sweden)

Yuan Ying

2017-01-01

Full Text Available The main function of steer-by-wire (SBW system are improving steering characteristics, security and stability of the vehicle. In this paper, the variable steering ratio of SBW system is analyzed, and the method of steering ratio based on fuzzy control and neural network are researched. In order to solve the actual working condition, the wheel angle may not reach the expected value, this paper establishes a twodegree-of-freedom (2-DOF vehicle model, and a Matlab/Simulink simulation model, in which a control strategy based on PID controller is put forward to control the front wheel steering angle. Simulation results show that proposed control strategy based on fuzzy neural network can effectively reduce lateral deviation and improve the handling stability and comfortability of the vehicle.

46 CFR 154.195 - Aluminum cargo tank: Steel enclosure.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Aluminum cargo tank: Steel enclosure. 154.195 Section... Equipment Hull Structure § 154.195 Aluminum cargo tank: Steel enclosure. (a) An aluminum cargo tank and its... the aluminum cargo tank must meet the steel structural standards of the American Bureau of Shipping...

46 CFR 182.620 - Auxiliary means of steering.

Science.gov (United States)

2010-10-01

... TONS) MACHINERY INSTALLATION Steering Systems § 182.620 Auxiliary means of steering. (a) Except as... personnel hazards during normal or heavy weather operation. (b) A suitable hand tiller may be acceptable as...

46 CFR 308.517 - Open Cargo Policy, Form MA-300.

Science.gov (United States)

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Open Cargo Policy, Form MA-300. 308.517 Section 308.517... Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.517 Open Cargo Policy, Form MA-300. The standard form of War Risk Open Cargo, Form MA-300, may be obtained from the American War Risk...

76 FR 51847 - Air Cargo Screening

Science.gov (United States)

2011-08-18

... apply to international inbound cargo. \\2\\ 74 FR 47672. The IFR provides detailed information on TSA's... Chain Management Professionals, Express Delivery and Logistics Association, International Air Transport... use in other countries for international inbound cargo. TSA Response: TSA is working closely with its...

Modelling and Simulation of a Hydrostatic Steering System for Agricultural Tractors

Directory of Open Access Journals (Sweden)

Barbara Zardin

2018-01-01

Full Text Available The steering system of a vehicle impacts on the vehicle performance, safety and on the driver’s comfort. Moreover, in off-road vehicles using hydrostatic steering systems, the energy dissipation also becomes a critical issue. These aspects push and motivate innovation, research and analysis in the field of agricultural tractors. This paper proposes the modelling and analysis of a hydrostatic steering system for an agricultural tractor to calculate the performance of the system and determine the influence of its main design parameters. The focus here is on the driver’s steering feel, which can improve the driver’s behavior reducing unnecessary steering corrections during the working conditions. The hydrostatic steering system is quite complex and involves a hydraulic circuit and a mechanical mechanism to transmit the steering to the vehicle tires. The detailed lumped parameters model here proposed allows to simulate the dynamic behavior of the steering system and to both enhance the understanding of the system and to improve the design through parameters sensitivity analysis.

46 CFR 151.25-2 - Cargo handling space.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo handling space. 151.25-2 Section 151.25-2 Shipping... BULK LIQUID HAZARDOUS MATERIAL CARGOES Environmental Control § 151.25-2 Cargo handling space. Pump rooms, compressor rooms, refrigeration rooms, heating rooms, instrument rooms or other closed spaces...

The Cargo

DEFF Research Database (Denmark)

Birch, Thomas; Charlton, Michael F.; Biggs, Lynn

2014-01-01

The Gresham ship was carrying a substantial cargo of different metals when she sank, representing a considerable financial loss. Reports record that some of that material recovered in 1846 included 2700 tin ingots, lead pigs and iron bars (British Archaeological Association, 1847, 361......). The excavations in 2003–4 found a further three stamped lead ingots, five tin ingots and 42 iron bars from the hull area of the ship. The size of the original cargo remains unknown, though the estimated capacity of the ship, perhaps some 150 tons, provides an indication. In this chapter, the assemblage of lead...... and tin ingots and the bar iron will be described, the production processes established and the innovative analytical evidence for the provenance of the metals discussed. The research presented here suggests that lead ingots were from Derbyshire, the tin from Cornwall, but the bar iron seems...

Live animal assessments of rump fat and muscle score in Angus cows and steers using 3-dimensional imaging.

Science.gov (United States)

McPhee, M J; Walmsley, B J; Skinner, B; Littler, B; Siddell, J P; Cafe, L M; Wilkins, J F; Oddy, V H; Alempijevic, A

2017-04-01

curvature as a form of representing body shape. A data-driven model from shape to trait has established a proof of concept using optimized machine learning techniques to assess P8 fat and MS in Angus cows and steers.

46 CFR 154.1850 - Entering cargo handling spaces.

Science.gov (United States)

2010-10-01

..., compressors, and compressor motors. (2) Gas-dangerous cargo control spaces. (3) Other spaces containing cargo... cargo handling spaces. (a) The master shall ensure that the ventilation system under § 154.1200 is in... ventilation system, is posted outside of each space under paragraph (a) of this section. (c) The master shall...

Retrospective Review of Pectoralis Major Ruptures in Rodeo Steer Wrestlers

Directory of Open Access Journals (Sweden)

Breda H. F. Lau

2013-01-01

Full Text Available Background. Pectoralis major tendon ruptures have been reported in the literature as occupational injuries, accidental injuries, and sporting activities. Few cases have been reported with respect to rodeo activities. Purpose. To describe a series of PM tendon ruptures in professional steer wrestlers. Study Design. Case series, level of evidence, 4. Methods. A retrospective analysis of PM ruptures in a steer wrestling cohort was performed. Injury data between 1992 and 2008 were reviewed using medical records from the University of Calgary Sport Medicine Center. Results. Nine cases of pectoralis major ruptures in professional steer wrestlers were identified. Injuries occurred during the throwing phase of the steer or while breaking a fall. All athletes reported unexpected or abnormal behavior of the steer that contributed to the mechanism of injury. Seven cases were surgically repaired, while two cases opted for nonsurgical intervention. Eight cases reported successful return to competition following the injury. Conclusion. Steer wrestlers represent a unique cohort of PM rupture case studies. Steer wrestling is a demanding sport that involves throwing maneuvers that may predispose the muscle to rupture. All cases demonstrated good functional outcomes regardless of surgical or non-surgical treatment.

Coin Tossing Explains the Activity of Opposing Microtubule Motors on Phagosomes.

Science.gov (United States)

Sanghavi, Paulomi; D'Souza, Ashwin; Rai, Ashim; Rai, Arpan; Padinhatheeri, Ranjith; Mallik, Roop

2018-05-07

How the opposing activity of kinesin and dynein motors generates polarized distribution of organelles inside cells is poorly understood and hotly debated [1, 2]. Possible explanations include stochastic mechanical competition [3, 4], coordinated regulation by motor-associated proteins [5-7], mechanical activation of motors [8], and lipid-induced organization [9]. Here, we address this question by using phagocytosed latex beads to generate early phagosomes (EPs) that move bidirectionally along microtubules (MTs) in an in vitro assay [9]. Dynein/kinesin activity on individual EPs is recorded as real-time force generation of the motors against an optical trap. Activity of one class of motors frequently coincides with, or is rapidly followed by opposite motors. This leads to frequent and rapid reversals of EPs in the trap. Remarkably, the choice between dynein and kinesin can be explained by the tossing of a coin. Opposing motors therefore appear to function stochastically and independently of each other, as also confirmed by observing no effect on kinesin function when dynein is inhibited on the EPs. A simple binomial probability calculation based on the geometry of EP-microtubule contact explains the observed activity of dynein and kinesin on phagosomes. This understanding of intracellular transport in terms of a hypothetical coin, if it holds true for other cargoes, provides a conceptual framework to explain the polarized localization of organelles inside cells. Copyright © 2018 The Author(s). Published by Elsevier Ltd.. All rights reserved.

78 FR 68784 - Cargo Securing Manuals

Science.gov (United States)

2013-11-15

.../Circ.) 1352 (``Cargo Stowage and Securing (CSS Code) Annex 14 Guidance on Providing Safe Working... Providing Safe Working Conditions for the Securing of Containers'') of the IMO 2010 CSS Code. A cargo safe.... Indian Tribal Governments K. Energy Effects L. Technical Standards M. Environment I. Public Participation...

Creation of quantum steering by interaction with a common bath

Science.gov (United States)

Sun, Zhe; Xu, Xiao-Qiang; Liu, Bo

2018-05-01

By applying the hierarchy equation method, we computationally study the creation of quantum steering in a two-qubit system interacting with a common bosonic bath. The calculation does not adopt conventional approximate approaches, such as the Born, Markov, rotating-wave, and other perturbative approximations. Three kinds of quantum steering, i.e., Einstein-Podolsky-Rosen steering (EPRS), temporal steering (TS), and spatiotemporal steering (STS), are considered. Since the initial state of the two qubits is chosen as a product state, there does not exist EPRS at the beginning. During the evolution, we find that STS as well as EPRS are generated at the same time. An inversion relationship between STS and TS is revealed. By varying the system-bath coupling strength from weak to ultrastrong regimes, we find the nonmonotonic dependence of STS, TS, and EPRS on the coupling strength. It is interesting to study the dynamics of the three kinds of quantum steering by using an exactly numerical method, which is not considered in previous researches.

Characterizing Density and Complexity of Imported Cargos

Energy Technology Data Exchange (ETDEWEB)

Birrer, Nathaniel [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Divin, Charles [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Glenn, Steven [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Martz, Harry [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2017-08-01

X-ray inspection systems are used to detect radiological and nuclear threats in imported cargo. In order to better understand performance of these systems, system imaging capabilities and the characteristics of imported cargo need to be determined. This project involved calculation of the modulation transfer function as a metric of system imaging performance and a study of the density and inhomogeneity of imported cargos, which have been shown to correlate with human analysts, threat detection performance.

A theoretical model of speed-dependent steering torque for rolling tyres

Science.gov (United States)

Wei, Yintao; Oertel, Christian; Liu, Yahui; Li, Xuebing

2016-04-01

It is well known that the tyre steering torque is highly dependent on the tyre rolling speed. In limited cases, i.e. parking manoeuvre, the steering torque approaches the maximum. With the increasing tyre speed, the steering torque decreased rapidly. Accurate modelling of the speed-dependent behaviour for the tyre steering torque is a key factor to calibrate the electric power steering (EPS) system and tune the handling performance of vehicles. However, no satisfactory theoretical model can be found in the existing literature to explain this phenomenon. This paper proposes a new theoretical framework to model this important tyre behaviour, which includes three key factors: (1) tyre three-dimensional transient rolling kinematics with turn-slip; (2) dynamical force and moment generation; and (3) the mixed Lagrange-Euler method for contact deformation solving. A nonlinear finite-element code has been developed to implement the proposed approach. It can be found that the main mechanism for the speed-dependent steering torque is due to turn-slip-related kinematics. This paper provides a theory to explain the complex mechanism of the tyre steering torque generation, which helps to understand the speed-dependent tyre steering torque, tyre road feeling and EPS calibration.

Optimization of the air cargo supply chain

Directory of Open Access Journals (Sweden)

María Pérez Bernal

2012-10-01

Full Text Available Purpose: This paper aims to evaluate and optimize the various operations within the air cargo chain. It pursues to improve the efficiency of the air cargo supply chain and to provide more information to the decision-makers to optimize their fields.Design/methodology/approach: The method used is a process simulation modelling software, WITNESS, which provides information to the decision-makers about the most relevant parameters subject to optimization. The input for the simulation is obtained from a qualitative analysis of the air cargo supply chain with the involved agents and from a study of the external trade by air mode, given that their behaviour depend on the location. The case study is focused on a particular location, the Case of Zaragoza Airport (Spain.Findings: This paper demonstrates that efficiency of the air cargo supply chain can increase by leveraging several parameters such as bottlenecks, resources or warehouses.Originality/value: It explores the use of a simulation modeling software originally intended for manufacturing processes and extended to support decision making processes in the area of air cargo.

46 CFR 154.560 - Cargo hose: Prototype test.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo hose: Prototype test. 154.560 Section 154.560... Hose § 154.560 Cargo hose: Prototype test. (a) Each cargo hose must be of a type that passes a prototype test at a pressure of at least five times its maximum working pressure at or below the minimum...

Electronic differential control of 2WD electric vehicle considering steering stability

Science.gov (United States)

Hua, Yiding; Jiang, Haobin; Geng, Guoqing

2017-03-01

Aiming at the steering wheel differential steering control technology of rear wheel independent driving electric wheel, considering the assisting effect of electronic differential control on vehicle steering, based on the high speed steering characteristic of electric wheel car, the electronic differential speed of auxiliary wheel steering is also studied. A yaw moment control strategy is applied to the vehicle at high speed. Based on the vehicle stability reference value, yaw rate is used to design the fuzzy controller to distribute the driving wheel torque. The simulation results show that the basic electronic differential speed function is realized based on the yaw moment control strategy, while the vehicle stability control is improved and the driving safety is enhanced. On the other hand, the torque control strategy can also assist steering of vehicle.

46 CFR 154.1810 - Cargo manual.

Science.gov (United States)

2010-10-01

... with inert gas and air. (13) A description of hull and cargo tank temperature monitoring systems. (14... vaporization system. (v) Gas main system. (vi) Cargo tank or compressor relief system and blocked liquid or gas relief system. (vii) Inerting system. (viii) Boil-off gas compressor or reliquefaction system. (ix) Gas...

Development of active rear steer actuator. Development of four wheel steer actuator for active safety; Active rear steer actuator no kaihatsu. Yobo anzen ni muketa 4WS actuator no kaihatsu

Energy Technology Data Exchange (ETDEWEB)

Yamanaka, T [Aisin Seiki Co. Ltd., Aichi (Japan)

1997-10-01

Recently, ecology, energy saving and safety have become important issues. And Active Safety is spotlighted in vehicle control area. Many researches and developments on four wheel steer system have been done to improve vehicle stability. We have developed the Active Rear Steer system with electromechanical Actuator, which is mass-productive, compact, and high response and durable. 10 figs., 5 tabs.

supplementation of energy and/or protein to steers grazing summer ...

African Journals Online (AJOL)

Some aspects of the effect of supplementary energy and/or protein, strategically ptovided to steers on summer veld, were investi gated. 40 Friesland steers in the age ... was posed whether the strategic provision of energy and or protein to steers grazing .... Definitions and abbreviotions was determined after cooling for 24 h ...

46 CFR 78.47-55 - Instructions for changing steering gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 3 2010-10-01 2010-10-01 false Instructions for changing steering gear. 78.47-55... OPERATIONS Markings for Fire and Emergency Equipment, Etc. § 78.47-55 Instructions for changing steering gear..., relating in order, the different steps to be taken in changing to the emergency steering gear. Each clutch...

Genuine Multipartite Einstein-Podolsky-Rosen Steering

Science.gov (United States)

He, Q. Y.; Reid, M. D.

2013-12-01

We develop the concept of genuine N-partite Einstein-Podolsky-Rosen (EPR) steering. This nonlocality is the natural multipartite extension of the original EPR paradox. Useful properties emerge that are not guaranteed for genuine multipartite entangled states. In particular, there is a close link with the task of one-sided, device-independent quantum secret sharing. We derive inequalities to demonstrate multipartite EPR steering for Greenberger-Horne-Zeilinger and Gaussian continuous variable states in loophole-free scenarios.

System for inspection of stacked cargo containers

Science.gov (United States)

Derenzo, Stephen [Pinole, CA

2011-08-16

The present invention relates to a system for inspection of stacked cargo containers. One embodiment of the invention generally comprises a plurality of stacked cargo containers arranged in rows or tiers, each container having a top, a bottom a first side, a second side, a front end, and a back end; a plurality of spacers arranged in rows or tiers; one or more mobile inspection devices for inspecting the cargo containers, wherein the one or more inspection devices are removeably disposed within the spacers, the inspection means configured to move through the spacers to detect radiation within the containers. The invented system can also be configured to inspect the cargo containers for a variety of other potentially hazardous materials including but not limited to explosive and chemical threats.

19 CFR 122.117 - Requirements for transit air cargo transport.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 1 2010-04-01 2010-04-01 false Requirements for transit air cargo transport. 122... Requirements for transit air cargo transport. (a) Transportation—(1) Port to port. Transit air cargo may be... surface carrier for transport. Otherwise, all shipments on the transit air cargo manifest shall be...

46 CFR 97.37-33 - Instructions for changing steering gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Instructions for changing steering gear. 97.37-33... steering gear. (a) Instructions in at least 1/2 inch letters and figures shall be posted in the steering... gear. Each clutch, gear, wheel, lever, valve, or switch which is used during the changeover shall be...

Revenue management of air cargo service in theory and practice

Science.gov (United States)

Budiarto, S.; Putro, H. P.; Pradono, P.; Yudoko, G.

2018-05-01

This study examines the air cargo service by comparing existing theories from previous research with the conditions on the ground. The object of the study is focused on the freight forwarder and the airport management. This study reviews the models and results of previous research that will be summarized and used to identify any issues related to the characteristics of air cargo operational services, as well as observing and monitoring literature with airlines, shipping companies, and airport management to explore and see the gap between prior research and implementation of its process in the air cargo service. The first phase in this study is to provide an overview of the air cargo industry. The second phase analyzes the characteristic differences between air cargo services and air passenger operating services. And the third phase is a literary bibliography study of air cargo operations, where the focus is on the studies using quantitative models from the perspective of the object of the study, which is the optimization of revenue management on air cargo services. From the results of the study, which is based on the gap between theory and practice, new research opportunities which are related to management of air cargo service revenue in the form of model development are found by adding booking timelines aspects of cargo that can affect the revenue of cargo airline companies and airports.

Applications of EPR steering in quantum teleportation and NOON states

Science.gov (United States)

Zárate, Laura Rosales

2018-04-01

Einstein-Podolsky-Rosen (EPR) steering refers to the type of correlations described in the EPR paradox, where one observer seems to affect ("steer") the state of other observer by using local measurements. There have been several works regarding characterization and quantification of EPR steering. One characteristic of this non-local correlation is that it can be asymmetric, while entanglement is symmetric. This asymmetric property is relevant for potential applications of EPR steering to quantum information, in particular to quantum cryptography and quantum teleportation. This latter refers to the process where one observer sends an unknown quantum state to Bob, who is in a different location. They communicate by classical means. Here we will show that EPR steering is a necessary resource to obtain secure continuous variable teleportation. We will also consider NOON states, which is an example of an entangled state. For this state, we will present a steering signature. This contribution reviews the work derived in Refs. [1] and [2], which was presented as an invited talk in ELAF 2017.

Monogamy inequalities for the Einstein-Podolsky-Rosen paradox and quantum steering

Science.gov (United States)

Reid, M. D.

2013-12-01

Monogamy inequalities for the way bipartite Einstein-Podolsky-Rosen (EPR) steering can be distributed among N systems are derived. One set of inequalities is based on witnesses with two measurement settings, and may be used to demonstrate correlation of outcomes between two parties, that cannot be shared with more parties. It is shown that the monogamy for steering is directional. Two parties cannot independently demonstrate steering of a third system, using the same two-setting steering witness, but it is possible for one party to steer two independent systems. This result explains the monogamy of two-setting Bell inequality violations and the sensitivity of the continuous variable (CV) EPR criterion to losses on the steering party. We generalize to m settings. A second type of monogamy relation gives the quantitative amount of sharing possible, when the number of parties is less than or equal to m, and takes a form similar to the Coffman-Kundu-Wootters relation for entanglement. The results enable characterization of the tripartite steering for CV Gaussian systems and qubit Greenberger-Horne-Zeilinger and W states.

46 CFR 153.436 - Heat transfer fluids: compatibility with cargo.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Heat transfer fluids: compatibility with cargo. 153.436... Equipment Cargo Temperature Control Systems § 153.436 Heat transfer fluids: compatibility with cargo. A heat transfer fluid separated from the cargo by only one wall (for example, the heat transfer fluid in a coil...

Evaluation of flexible endoscope steering using haptic guidance

NARCIS (Netherlands)

Reilink, Rob; Stramigioli, Stefano; Kappers, Astrid M L; Misra, Sarthak

Background: Steering the tip of a flexible endoscope relies on the physician's dexterity and experience. For complex flexible endoscopes, conventional controls may be inadequate. Methods: A steering method based on a multi-degree-of-freedom haptic device is presented. Haptic cues are generated based

Evaluation of flexible endoscope steering using haptic guidance

NARCIS (Netherlands)

Reilink, Rob; Stramigioli, Stefano; Kappers, Astrid M.L.; Misra, Sarthak

2011-01-01

Background - Steering the tip of a flexible endoscope relies on the physician’s dexterity and experience. For complex flexible endoscopes, conventional controls may be inadequate. Methods - A steering method based on a multi-degree-of-freedom haptic device is presented. Haptic cues are generated

Determinants of Operational Efficiency at Chemical Cargo Terminals

Directory of Open Access Journals (Sweden)

T.A. Gúlcan

2014-06-01

Full Text Available In today’s globalized world, one of the requirements of global supply chains is efficient transportation systems. Approximately 80 per cent of world merchandise trade carried by sea and handled by ports worldwide. For this reason, maritime transport has the strategic economic importance. Loading of oil and gas has the biggest share (%30 in commodities carried by sea and 2.9 billion tons oil and gas loaded to ship in 2013. This study is focus on chemical cargo terminals which is a special terminal form where high and international levels of safety and quality elements applied. Unlike conventional bulk cargo and container cargo operations, chemical cargo operations include own priorities, applications, and the evaluation criteria. The aim of this study is to perform a qualitative research to determine the factors affecting the operational efficiency of ship, berth and warehousing operations in chemical cargo terminals.

Demonstration of Multisetting One-Way Einstein-Podolsky-Rosen Steering in Two-Qubit Systems

Science.gov (United States)

Xiao, Ya; Ye, Xiang-Jun; Sun, Kai; Xu, Jin-Shi; Li, Chuan-Feng; Guo, Guang-Can

2017-04-01

Einstein-Podolsky-Rosen (EPR) steering describes the ability of one party to remotely affect another's state through local measurements. One of the most distinguishable properties of EPR steering is its asymmetric aspect. Steering can work in one direction but fail in the opposite direction. This type of one-way steering, which is different from the symmetry concepts of entanglement and Bell nonlocality, has garnered much interest. However, an experimental demonstration of genuine one-way EPR steering in the simplest scenario, i.e., one that employs two-qubit systems, is still lacking. In this Letter, we experimentally demonstrate one-way EPR steering with multimeasurement settings for a class of two-qubit states, which are still one-way steerable even with infinite settings. The steerability is quantified by the steering radius, which represents a necessary and sufficient steering criterion. The demonstrated one-way steering in the simplest bipartite quantum system is of fundamental interest and may provide potential applications in one-way quantum information tasks.

Study on a New Steering Mechanism for Point-the-Bit Rotary Steerable System

Directory of Open Access Journals (Sweden)

Yuanzhi Li

2014-02-01

Full Text Available This paper presents a novel steering mechanism embedded in a point-the-bit rotary steerable system (RSS for oilfield exploitation. The new steering mechanism adopts a set of universal joints to alleviate the high alternative strain on drilling mandrel and employs a specially designed planetary gear small tooth number difference (PGSTD to achieve directional steering. Its principle and characteristics are explained and examined through a series of analyses. First, the eccentric displacement vector of the offset point on the drilling mandrel is formulated and kinematic solutions are established. Next, structural design for the new steering mechanism is addressed. Then, procedures and program architectures for simulating offset state of the drilling mandrel and motion trajectory of the whole steering mechanism are presented. After that, steering motion simulations of the new steering mechanism for both 2D and 3D well trajectories are then performed by combining LabVIEW and SolidWorks. Finally, experiments on the steering motion control of the new steering mechanism prototype are carried out. The simulations and experiments reveal that the steering performance of the new steering mechanism is satisfied. The research can provide good guidance for further research and engineering application of the point-the-bit RSS.

Indirect estimation of radioactivity in containerized cargo

International Nuclear Information System (INIS)

Jarman, K.D.; Scherrer, C.; Smith, L.E.; Chilton, L.K.; Anderson, K.K.; Ressler, J.J.; Trease, L.L.

2011-01-01

Naturally occurring radioactive material in containerized cargo challenges the state of the art in national and international efforts to detect illicit nuclear and radiological material in transported containers. Current systems are being evaluated and new systems envisioned to provide the high probability of detection necessary to thwart potential threats, combined with extremely low nuisance and false alarm rates necessary to maintain the flow of commerce impacted by the enormous volume of commodities imported in shipping containers. Maintaining flow of commerce also means that inspection must be rapid, requiring relatively non-intrusive, indirect measurements of cargo from outside containers to the extent possible. With increasing information content in such measurements, it is natural to ask how the information might be combined to improve detection. Toward this end, we present an approach to estimating isotopic activity of naturally occurring radioactive material in cargo grouped by commodity type, combining container manifest data with radiography and gamma-ray spectroscopy aligned to location along the container. The heart of this approach is our statistical model of gamma-ray counts within peak regions of interest, which captures the effects of background suppression, counting noise, convolution of neighboring cargo contributions, and down-scattered photons to provide estimates of counts due to decay of specific radioisotopes in cargo alone. Coupled to that model, we use a mechanistic model of self-attenuated radiation flux to estimate the isotopic activity within cargo, segmented by location within each container, that produces those counts. We test our approach by applying it to a set of measurements taken at the Port of Seattle in 2006. This approach to synthesizing disparate available data streams and extraction of cargo characteristics, while relying on several simplifying assumptions and approximations, holds the potential to support improvement of

A novel mouse model with impaired dynein/dynactin function develops amyotrophic lateral sclerosis (ALS)-like features in motor neurons and improves lifespan in SOD1-ALS mice

NARCIS (Netherlands)

E. Teuling (Eva); V. van Dis (Vera); P. Wulf (Phebe); E.D. Haasdijk (Elize); A.S. Akhmanova (Anna); C.C. Hoogenraad (Casper); D. Jaarsma (Dick)

2008-01-01

textabstractAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative condition characterized by progressive motor neuron degeneration and muscle paralysis. Genetic evidence from man and mouse has indicated that mutations in the dynein/dynactin motor complex are correlated with motor neuron

Revisiting path steering for 3D manipulation tasks

NARCIS (Netherlands)

L. Liu (Lei); J.-B. Martens; R. van Liere (Robert)

2011-01-01

htmlabstractThe law of path steering, as proposed by Accot and Zhai, describes a quantitative relationship between human temporal performance and the path spatial characteristics. The steering law is formulated as a continuous goal crossing task, in which a large number of goals are crossed along

Revisiting path steering for 3D manipulation tasks

NARCIS (Netherlands)

L. Liu (Lei); J.-B. Martens; R. van Liere (Robert)

2010-01-01

htmlabstractThe law of path steering, as proposed by Accot and Zhai, describes a quantitative relationship between human temporal performance and the path spatial characteristics. The steering law is formulated as a continuous goal crossing task, in which a large number of goals are crossed along

46 CFR 28.885 - Cargo gear.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo gear. 28.885 Section 28.885 Shipping COAST GUARD... Aleutian Trade Act Vessels § 28.885 Cargo gear. (a) The safe working load (SWL) for the assembled gear... the load the gear is approved to lift, excluding the weight of the gear itself. (b) All wire rope...

Growth, carcass characteristics, and profitability of organic versus conventional dairy beef steers.

Science.gov (United States)

Bjorklund, E A; Heins, B J; Dicostanzo, A; Chester-Jones, H

2014-03-01

Bull calves (n=49), born at the University of Minnesota West Central Research and Outreach Center (Morris) between March and May 2011, were used to compare growth measurements and profitability of conventional and organic dairy steers. Calves were assigned to 1 of 3 replicated groups at birth: conventional (CONV; n=16), organic (pasture and concentrate; ORG; n=16), or organic grass only (GRS; n=17), and analysis of variables was on a pen basis. Breed groups of calves were Holstein (HO; n=9); Holsteins (n=11) maintained at 1964 breed average level; crossbreds (n=19) including combinations of HO, Montbéliarde, and Swedish Red; and crossbreds (n=10) including combinations of HO, Jersey, Swedish Red, and Normande. The CONV steers were fed a diet of 80% concentrate and 20% forage. The ORG steers were fed a diet of organic corn, organic corn silage, and at least 30% of their diet consisted of organic pasture during the grazing season. The GRS steers grazed pasture during the grazing season and were fed high-quality hay or hay silage during the nongrazing season. Intakes of a total mixed ration were recorded daily with herd management software. A profit function was defined to include revenues and expenses for beef value, feed intake, pasture intake, health cost, and yardage. The GRS (358.6 kg) steers had lesser total gains from birth to slaughter than ORG (429.6 kg) and CONV (534.5 kg) steers. Furthermore, the GRS (0.61 kg/d) steers had lesser average daily gain from birth compared with ORG (0.81 kg/d) and CONV (1.1 kg/d) steers. The GRS and ORG steers had smaller rib eye area (49.5 and 65.8 cm(2), respectively) compared with CONV (75.4 cm(2)) steers. For profitability, GRS steers had 43% greater profit than CONV steers due to organic beef price premiums and lower feed costs. On the other hand, ORG steers had substantially less profit than CONV steers. The higher cost of production for the ORG steers is due to the extreme high value of organic corn. The results of the

Intracellular Transport of Cargo in a Sub-diffusive Environment over an Explicit Cytoskeletal Network

Science.gov (United States)

Maelfeyt, Bryan; Gopinathan, Ajay

Intracellular transport occurs in nearly all eukaryotic cells, where materials such as proteins, lipids, carbohydrates, and nucleic acids travel to target locations through phases of passive, diffusion-based transport and active, motor-driven transport along filaments that make up the cell's cytoskeleton.We develop a computational model of the process with explicit cytoskeletal filament networks. In the active transport phase, cargo moves in straight lines along these filaments that are spread throughout the cell. To model the passive transport phase of cargo in the cytoplasm, where anomalous sub-diffusion is thought to take place, we implement a continuous-time random walk. We use this approach to provide a stepping stone to a predictive model where we can determine transport properties over a cytoskeletal network provided by experimental images of real filaments. We illustrate our approach by modeling the transport of insulin out of the cell and determining the impact of network geometry, anomalous sub-diffusion and motor number on the first-passage time distributions for insulin granules reaching their target destinations on the membrane.

Autonomous Shepherding Behaviors of Multiple Target Steering Robots.

Science.gov (United States)

Lee, Wonki; Kim, DaeEun

2017-11-25

This paper presents a distributed coordination methodology for multi-robot systems, based on nearest-neighbor interactions. Among many interesting tasks that may be performed using swarm robots, we propose a biologically-inspired control law for a shepherding task, whereby a group of external agents drives another group of agents to a desired location. First, we generated sheep-like robots that act like a flock. We assume that each agent is capable of measuring the relative location and velocity to each of its neighbors within a limited sensing area. Then, we designed a control strategy for shepherd-like robots that have information regarding where to go and a steering ability to control the flock, according to the robots' position relative to the flock. We define several independent behavior rules; each agent calculates to what extent it will move by summarizing each rule. The flocking sheep agents detect the steering agents and try to avoid them; this tendency leads to movement of the flock. Each steering agent only needs to focus on guiding the nearest flocking agent to the desired location. Without centralized coordination, multiple steering agents produce an arc formation to control the flock effectively. In addition, we propose a new rule for collecting behavior, whereby a scattered flock or multiple flocks are consolidated. From simulation results with multiple robots, we show that each robot performs actions for the shepherding behavior, and only a few steering agents are needed to control the whole flock. The results are displayed in maps that trace the paths of the flock and steering robots. Performance is evaluated via time cost and path accuracy to demonstrate the effectiveness of this approach.

Autonomous Shepherding Behaviors of Multiple Target Steering Robots

Directory of Open Access Journals (Sweden)

Wonki Lee

2017-11-01

Full Text Available This paper presents a distributed coordination methodology for multi-robot systems, based on nearest-neighbor interactions. Among many interesting tasks that may be performed using swarm robots, we propose a biologically-inspired control law for a shepherding task, whereby a group of external agents drives another group of agents to a desired location. First, we generated sheep-like robots that act like a flock. We assume that each agent is capable of measuring the relative location and velocity to each of its neighbors within a limited sensing area. Then, we designed a control strategy for shepherd-like robots that have information regarding where to go and a steering ability to control the flock, according to the robots’ position relative to the flock. We define several independent behavior rules; each agent calculates to what extent it will move by summarizing each rule. The flocking sheep agents detect the steering agents and try to avoid them; this tendency leads to movement of the flock. Each steering agent only needs to focus on guiding the nearest flocking agent to the desired location. Without centralized coordination, multiple steering agents produce an arc formation to control the flock effectively. In addition, we propose a new rule for collecting behavior, whereby a scattered flock or multiple flocks are consolidated. From simulation results with multiple robots, we show that each robot performs actions for the shepherding behavior, and only a few steering agents are needed to control the whole flock. The results are displayed in maps that trace the paths of the flock and steering robots. Performance is evaluated via time cost and path accuracy to demonstrate the effectiveness of this approach.

Drowsy Driver Detection via Steering Wheel

Directory of Open Access Journals (Sweden)

Herlina ABDUL RAHIM

2010-09-01

Full Text Available The main purpose of this project is to produce a safety system especially for fatigue car driver so as to prevent from accidents. The statistic on road fatality shows that human error constitute of 64.84 % road accidents fatality and 17.4 % due to technical factors. These systems encompassed the approach of hand pressure applied on the steering wheel. The steering will be installed with pressure sensors. At the same time these sensors can be used to measure gripping force while driving.

Experimental EPR-steering using Bell-local states

Science.gov (United States)

Saunders, D. J.; Jones, S. J.; Wiseman, H. M.; Pryde, G. J.

2010-11-01

The concept of `steering' was introduced in 1935 by Schrödinger as a generalization of the EPR (Einstein-Podolsky-Rosen) paradox. It has recently been formalized as a quantum-information task with arbitrary bipartite states and measurements, for which the existence of entanglement is necessary but not sufficient. Previous experiments in this area have been restricted to an approach that followed the original EPR argument in considering only two different measurement settings per side. Here we demonstrate experimentally that EPR-steering occurs for mixed entangled states that are Bell local (that is, that cannot possibly demonstrate Bell non-locality). Unlike the case of Bell inequalities, increasing the number of measurement settings beyond two-we use up to six-significantly increases the robustness of the EPR-steering phenomenon to noise.

Diverse mitotic functions of the cytoskeletal cross-linking protein Shortstop suggest a role in Dynein/Dynactin activity.

Science.gov (United States)

Dewey, Evan B; Johnston, Christopher A

2017-09-15

Proper assembly and orientation of the bipolar mitotic spindle is critical to the fidelity of cell division. Mitotic precision fundamentally contributes to cell fate specification, tissue development and homeostasis, and chromosome distribution within daughter cells. Defects in these events are thought to contribute to several human diseases. The underlying mechanisms that function in spindle morphogenesis and positioning remain incompletely defined, however. Here we describe diverse roles for the actin-microtubule cross-linker Shortstop (Shot) in mitotic spindle function in Drosophila Shot localizes to mitotic spindle poles, and its knockdown results in an unfocused spindle pole morphology and a disruption of proper spindle orientation. Loss of Shot also leads to chromosome congression defects, cell cycle progression delay, and defective chromosome segregation during anaphase. These mitotic errors trigger apoptosis in Drosophila epithelial tissue, and blocking this apoptotic response results in a marked induction of the epithelial-mesenchymal transition marker MMP-1. The actin-binding domain of Shot directly interacts with Actin-related protein-1 (Arp-1), a key component of the Dynein/Dynactin complex. Knockdown of Arp-1 phenocopies Shot loss universally, whereas chemical disruption of F-actin does so selectively. Our work highlights novel roles for Shot in mitosis and suggests a mechanism involving Dynein/Dynactin activation. © 2017 Dewey and Johnston. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

Chaos vibration of pinion and rack steering trapezoidal mechanism containing two clearances

Science.gov (United States)

Wei, Daogao; Wang, Yu; Jiang, Tong; Zheng, Sifa; Zhao, Wenjing; Pan, Zhijie

2017-08-01

The multi-clearances of breaking type steering trapezoidal mechanism joints influences vehicle steering stability. Hence, to ascertain the influence of clearance value on steering stability, this paper takes the steering mechanism of a certain vehicle type as a prototype that can be simplified into a planar six-bar linkage, then establishes the system dynamic differential equations after considering the two clearances of tie rods and the steering knuckle arms. The influence of the clearance parameters on the movement stability of the steering mechanism is studied using a numerical computation method. Results show that when the two clearances are equal, the planar movement of the tie rods changes from period-doubling to chaos as the clearances increase. When the two clearances are 0.25 mm and 1.5 mm respectively, the planar movements of the two side tie rods come into chaos, causing the steering stability to deteriorate. Moreover, with the increase of clearances, turning moment fluctuates more intensively and the peak value increases.

The Optimal Steering Control System using Imperialist Competitive Algorithm on Vehicles with Steer-by-Wire System

Directory of Open Access Journals (Sweden)

F. Hunaini

2015-03-01

Full Text Available Steer-by-wire is the electrical steering systems on vehicles that are expected with the development of an optimal control system can improve the dynamic performance of the vehicle. This paper aims to optimize the control systems, namely Fuzzy Logic Control (FLC and the Proportional, Integral and Derivative (PID control on the vehicle steering system using Imperialist Competitive Algorithm (ICA. The control systems are built in a cascade, FLC to suppress errors in the lateral motion and the PID control to minimize the error in the yaw motion of the vehicle. FLC is built has two inputs (error and delta error and single output. Each input and output consists of three Membership Function (MF in the form of a triangular for language term "zero" and two trapezoidal for language term "negative" and "positive". In order to work optimally, each MF optimized using ICA to get the position and width of the most appropriate. Likewise, in the PID control, the constant at each Proportional, Integral and Derivative control also optimized using ICA, so there are six parameters of the control system are simultaneously optimized by ICA. Simulations performed on vehicle models with 10 Degree Of Freedom (DOF, the plant input using the variables of steering that expressed in the desired trajectory, and the plant outputs are lateral and yaw motion. The simulation results showed that the FLC-PID control system optimized by using ICA can maintain the movement of vehicle according to the desired trajectory with lower error and higher speed limits than optimized with Particle Swarm Optimization (PSO.

Dose to drivers during drive-through cargo scanning using GEANT4 Monte Carlo simulation

International Nuclear Information System (INIS)

Gomes, Rogerio S.; Gomes, Joana D'Arc R.L.; Costa, Mara Lucia L.

2013-01-01

The use of radiation technologies to perform screening for cargo containers has been increased due to security issues, mainly, as a consequence of the United States (US) legislation which requires, from 2013, the scanning of all intermodal cargo containers which arrive at US ports. Currently, systems to cargo inspections, using accelerator-driven high energy X-rays, between 4 and 9 MeV, are available for scanning operations. It is expected that, in the future, the use of these systems will be widely spread on roads, ports and airports in Brazil. However, in order to improve the productivity and reduce the costs of acquisition, operation and maintenance these systems require that the driver drives its vehicle through irradiation area, in a situation where members of the public (the truck drivers) enter in controlled area and are deliberately exposed to high-energy beam. Some manufacturers justifies this procedure arguing that the drivers are exposed briefly, and only to the scattered beam, since there are safety systems in order to avoid that the drivers are exposed to direct beam. In this work, it is presented the preliminary results of Monte Carlo simulations concerning the dose of drivers during scanning operations, including the dose due to a failure of safety system, producing an exposure of drivers to the direct beam, as well as, an analysis of the justification of practice, mainly related to the drive-through operational procedure. (author)

Long-range cargo transport on crowded microtubules: The motor jamming mechanism

Science.gov (United States)

Rossi, Lucas W.; Radtke, Paul K.; Goldman, Carla

2014-05-01

The hopping model for cargo transport by molecular motors introduced in Goldman and Sena (2009), Goldman (2010) is extended here in order to incorporate the movement of cargo-motor complexes (C-MC). Hopping processes in this context express the possibility for cargo to be exchanged between neighboring motors at a microtubule where the transport takes place. Jamming of motors is essential for cargos to execute long-range movement in this way. Results from computer simulations accompanied by a mean-field analysis of the extended model confirm our previous analytical results and suggests that an interplay between cargo hopping and the movement of the C-MC’s would control the efficiency of cargo transfer and cargo delivery in these model systems.

Spectroscopic neutron radiography for a cargo scanning system

Energy Technology Data Exchange (ETDEWEB)

Rahon, Jill [Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States); Danagoulian, Areg, E-mail: aregjan@mit.edu [Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States); MacDonald, Thomas D. [Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States); Hartwig, Zachary S. [Plasma Science and Fusion Center, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States); Lanza, Richard C. [Department of Nuclear Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139 (United States)

2016-06-01

Detection of cross-border smuggling of illicit materials and contraband is a challenge that requires rapid, low-dose, and efficient radiographic technology. The work we describe here is derived from a technique which uses monoenergetic gamma rays from low energy nuclear reactions, such as {sup 11}B(d,nγ){sup 12}C, to perform radiographic analysis of shipping containers. Transmission ratios of multiple monoenergetic gamma lines resulting from several gamma producing nuclear reactions can be employed to detect materials of high atomic number (Z), the details of which will be described in a separate paper. Inherent in this particular nuclear reaction is the production of fast neutrons which could enable neutron radiography and further characterization of the effective-Z of the cargo, especially within the range of lower Z. Previous research efforts focused on the use of total neutron counts in combination with X-ray radiography to characterize the hydrogenous content of the cargo. We present a technique of performing transmitted neutron spectral analysis to reconstruct the effective Z and potentially the density of the cargo. This is made possible by the large differences in the energy dependence of neutron scattering cross-sections between hydrogenous materials and those of higher Z. These dependencies result in harder transmission spectra for hydrogenous cargoes than those of non-hydrogenous cargoes. Such observed differences can then be used to classify the cargo based on its hydrogenous content. The studies presented in this paper demonstrate that such techniques are feasible and can provide a contribution to cargo security, especially when used in concert with gamma radiography.

METHODS OF DIAGNOSTIC EFFECTIVENESS ORGANIZATIONAL CHANGES IN CARGO MOTOR TRANSPORTATION ORGANIZATIONS

Directory of Open Access Journals (Sweden)

Denis Sergeevich Antipov

2017-03-01

Full Text Available The article propose methods of diagnostic effectiveness organizational changes in cargo motor transportation organizations. The article contain the main results approbation of the developed methods of diagnostic effectiveness organizational changes in cargo motor transportation organizations in 37 cargo motor transportation organizations of Saint-Petersburg and the Leningrad Region. Constructed diagrams of conformity specific weights resulting from organizational changes in cargo motor transportation organizations. Presents diagrams effectiveness organizational changes in cargo motor transportation organizations at every stages of the life cycle. The goal of the present paper is to development methods of diagnostic effectiveness organizational changes in cargo motor transportation organizations.

Self-testing through EPR-steering

International Nuclear Information System (INIS)

Šupić, Ivan; Hoban, Matty J

2016-01-01

The verification of quantum devices is an important aspect of quantum information, especially with the emergence of more advanced experimental implementations of quantum computation and secure communication. Within this, the theory of device-independent robust self-testing via Bell tests has reached a level of maturity now that many quantum states and measurements can be verified without direct access to the quantum systems: interaction with the devices is solely classical. However, the requirements for this robust level of verification are daunting and require high levels of experimental accuracy. In this paper we discuss the possibility of self-testing where we only have direct access to one part of the quantum device. This motivates the study of self-testing via EPR-steering, an intermediate form of entanglement verification between full state tomography and Bell tests. Quantum non-locality implies EPR-steering so results in the former can apply in the latter, but we ask what advantages may be gleaned from the latter over the former given that one can do partial state tomography? We show that in the case of self-testing a maximally entangled two-qubit state, or ebit, EPR-steering allows for simpler analysis and better error tolerance than in the case of full device-independence. On the other hand, this improvement is only a constant improvement and (up to constants) is the best one can hope for. Finally, we indicate that the main advantage in self-testing based on EPR-steering could be in the case of self-testing multi-partite quantum states and measurements. For example, it may be easier to establish a tensor product structure for a particular party’s Hilbert space even if we do not have access to their part of the global quantum system. (paper)

Research on Sliding Mode Control for Steer-by-Wire System in Forklift

Directory of Open Access Journals (Sweden)

Huang Jun-Jie

2017-01-01

Full Text Available Aiming at steering stability and wheel angle tracking of steer-by-wire (SBW three wheeled forklift, steering dynamic model and SBW system mathematical model of three wheeled forklift are established. A control strategy for the ideal transmission ratio is introduced based on this model, which ensures forklift steering gain invariant. A sliding mode controller can then be designed based on the bound information of uncertain system parameters, uncertain self-aligning torque, and external disturbances. The results of simulation show the control strategies above can effectively reduce the sideslip angle when the forklift is steering and improve the sensitivity and stability of the steering forklift; at the same time can effectively restrain the parameter perturbation of internal system and external disturbance, which improves the tracking performance of the wheel angle.

Strong Einstein-Podolsky-Rosen steering with unconditional entangled states

Science.gov (United States)

Steinlechner, Sebastian; Bauchrowitz, Jöran; Eberle, Tobias; Schnabel, Roman

2013-02-01

In 1935 Schrödinger introduced the terms entanglement and steering in the context of the famous gedanken experiment discussed by Einstein, Podolsky, and Rosen (EPR). Here, we report on a sixfold increase of the observed EPR-steering effect with regard to previous experiments, as quantified by the Reid criterion. We achieved an unprecedented low conditional variance product of about 0.04<1, where 1 is the upper bound below which steering is demonstrated. The steering effect was observed on an unconditional two-mode-squeezed entangled state that contained a total vacuum state contribution of less than 8%, including detection imperfections. Together with the achieved high interference contrast between the entangled state and a bright coherent laser field, our state is compatible with efficient applications in high-power laser interferometers and fiber-based networks for entanglement distribution.

Cargo liability regimes

Science.gov (United States)

2001-01-01

There are at present at least three international regimes of maritime cargo liability in force in different countries of the world - the original Hague rules (1924), the updated version known as the Hague-Visby rules (1968, further amended 1979), and...

"Choice of Air Cargo Transshipment Airport: An Application to Air Cargo Traffic to/from Northeast Asia"

OpenAIRE

Hiroshi Ohashi; Tae-Seung Kim; Tae Hoon Oum; Chunyan Yu

2004-01-01

Based on a unique data set of 760 air cargo transshipment routings to/from the Northeast Asian region in 2000, this paper applies an aggregate form of multinomial logit model to identify the critical factors influencing air cargo transshipment route choice decisions. The analysis focuses on the trade-off between monetary cost and time cost while considering other variables relevant for choice of transshipment airport. The estimation method considers the presence of unobserved attributes, and ...

The impact of force feedback level on steering performance

NARCIS (Netherlands)

Anand, S.; Terken, J.; Hogema, J.H.

2013-01-01

Steer-by-wire systems provide designers the ability to customize and personalize force feedback on the steering wheel, based on individual preferences. Earlier studies using subjective responses have shown that there are individual differences in preferences for force feedback. It has also been

46 CFR 308.521 - Application for Open Cargo Policy, Form MA-301.

Science.gov (United States)

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Application for Open Cargo Policy, Form MA-301. 308.521... RISK INSURANCE War Risk Cargo Insurance Ii-Open Policy War Risk Cargo Insurance § 308.521 Application for Open Cargo Policy, Form MA-301. The standard form of application for a War Risk Open Cargo Policy...

Risk management algorithm for rear-side collision avoidance using a combined steering torque overlay and differential braking

Science.gov (United States)

Lee, Junyung; Yi, Kyongsu; Yoo, Hyunjae; Chong, Hyokjin; Ko, Bongchul

2015-06-01

This paper describes a risk management algorithm for rear-side collision avoidance. The proposed risk management algorithm consists of a supervisor and a coordinator. The supervisor is designed to monitor collision risks between the subject vehicle and approaching vehicle in the adjacent lane. An appropriate criterion of intervention, which satisfies high acceptance to drivers through the consideration of a realistic traffic, has been determined based on the analysis of the kinematics of the vehicles in longitudinal and lateral directions. In order to assist the driver actively and increase driver's safety, a coordinator is designed to combine lateral control using a steering torque overlay by motor-driven power steering and differential braking by vehicle stability control. In order to prevent the collision while limiting actuator's control inputs and vehicle dynamics to safe values for the assurance of the driver's comfort, the Lyapunov theory and linear matrix inequalities based optimisation methods have been used. The proposed risk management algorithm has been evaluated via simulation using CarSim and MATLAB/Simulink.

EVALUATION OF A CONCEPTUAL VEHICLE STEERING SYSTEM FOR INDEPENDENT WHEEL CONTROL

Directory of Open Access Journals (Sweden)

Ryszard BUCHALIK

2017-03-01

Full Text Available This paper presents a brief description of an unconventional steering system involving electronic stability control and its influence on vehicle motion. The proposed configuration enables individual changes in steering angle for each single wheel, in contrast to the mechanical linkage solution. An analysis of vehicle behaviour during emergency braking on a heterogeneous surface is conducted, especially with regard to the undesirable rotation of the vehicle body. The benefits of using this active steering system, implemented in the steer-by-wire mode, are characterized, while the problems for further consideration and the potential benefits of such a solution are described.

Robotic needle steering: design, modeling, planning, and image guidance

NARCIS (Netherlands)

Cowan, Noah J.; Goldberg, Ken; Chirikjian, Gregory S.; Fichtinger, Gabor; Alterovitz, Ron; Reed, Kyle B.; Kallem, Vinutha; Misra, Sarthak; Park, Wooram; Okamura, Allison M.; Rosen, Jacob; Hannaford, Blake; Satava, Richard M.

2010-01-01

This chapter describes how advances in needle design, modeling, planning, and image guidance make it possible to steer flexible needles from outside the body to reach specified anatomical targets not accessible using traditional needle insertion methods. Steering can be achieved using a variety of

Multiquantum well beam-steering device for laser satellite communication

Science.gov (United States)

Lahat, Roee; Levy, Itamar; Shlomi, Arnon

2002-01-01

With the increasing interest in laser satellite communications, new methods are sought to solve the existing problems of accurate and rapid laser beam deflection. Current solutions in the form of galvanometers or piezo fast steering mirrors with one or two degrees of freedom are bulky, power-consuming and slow. The Multi-Quantum Well (MQW) is a semiconductor device with unique potential to steer laser beams without any moving parts. We have conducted a preliminary evaluation of the potential application of the MQW as a laser beam-steering device for laser satellite communication, examining the performance of critical parameters for this type of communications.

Investigate existing non-intrusive (NII) technologies for port cargo inspections.

Science.gov (United States)

2011-09-01

"The quantity of cargo handled by United States ports has increased significantly in recent years. Based on : 2004 data, almost 2.7 billion tons of cargo passed through the ports in one year. To protect the U.S., all of this : cargo must be inspected...

33 CFR 401.76 - In-transit cargo.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false In-transit cargo. 401.76 Section... TRANSPORTATION SEAWAY REGULATIONS AND RULES Regulations Toll Assessment and Payment § 401.76 In-transit cargo... the Seaway Transit Declaration Form, but is deemed to be ballast and not subject to toll assessment. ...

Analytical solution of the problem of acceleration of cargo by a bridge crane with constant acceleration at elimination of swings of a cargo rope

Science.gov (United States)

Korytov, M. S.; Shcherbakov, V. S.; Titenko, V. V.

2018-01-01

Limitation of the swing of the bridge crane cargo rope is a matter of urgency, as it can significantly improve the efficiency and safety of the work performed. In order to completely dampen the pendulum swing after the break-up of a bridge or a bridge-crane freight cart to maximum speed, it is necessary, in the normal repulsion control of the electric motor, to split the process of dispersion into a minimum of three gaps. For a dynamic system of swinging of a bridge crane on a flexible cable hanger in a separate vertical plane, an analytical solution was obtained to determine the temporal dependence of the cargo rope angle relative to the gravitational vertical when the cargo suspension point moves with constant acceleration. The resulting analytical dependence of the cargo rope angle and its first derivative can break the process of dispersing the cargo suspension point into three stages of dispersal and braking with various accelerations and enter maximum speed of movement of the cargo suspension point. In doing so, the condition of eliminating the swings of the cargo rope relative to the gravitational vertical is fulfilled. Provides examples of the maximum speed output constraints-to-time when removing the rope swing.

Reducing Digging Losses by Using Automated Steering to Plant and Invert Peanuts

Directory of Open Access Journals (Sweden)

George Vellidis

2014-07-01

Full Text Available GPS guidance of farm machinery has been increasingly adopted by farmers because of the perceived gains in efficiency that it provides. In the southeastern USA one of the reasons farmers adopt GPS guidance, and specifically automated steering (auto-steer, is that it can theoretically result in large yield gains when used to plant and invert peanuts—one of the region’s most important crops. The goal of our study was to quantify the yield benefit of using real time kinematic (RTK-based auto-steer to plant and invert peanuts under a variety of terrain conditions. Yield benefits result from reduced digging losses. The study was conducted for two consecutive years (2010 and 2011 on a private farm in Georgia, USA. When all data are grouped together, auto-steer outperformed conventional by 579 kg/ha in 2010 and 451 kg/ha in 2011. We also evaluated the performance of auto-steer under different curvature conditions using low, medium, and high curvature rows. The results showed that auto-steer outperformed conventional under all curvature by a minimum of 338 kg/ha. Finally, we evaluated passive implement guidance in combination with auto-steer and found that it holds tremendous potential for further reducing digging losses. In many cases, auto-steer will pay for itself within a year.

Structural insights into microtubule doublet interactions inaxonemes

Energy Technology Data Exchange (ETDEWEB)

Downing, Kenneth H.; Sui, Haixin

2007-06-06

Coordinated sliding of microtubule doublets, driven by dynein motors, produces periodic beating of the axoneme. Recent structural studies of the axoneme have used cryo-electron tomography to reveal new details of the interactions among some of the multitude of proteins that form the axoneme and regulate its movement. Connections among the several sets of dyneins, in particular, suggest ways in which their actions may be coordinated. Study of the molecular architecture of isolated doublets has provided a structural basis for understanding the doublet's mechanical properties that are related to the bending of the axoneme, and has also offered insight into its potential role in the mechanism of dynein activity regulation.

Modeling of Driver Steering Operations in Lateral Wind Disturbances toward Driver Assistance System

Science.gov (United States)

Kurata, Yoshinori; Wada, Takahiro; Kamiji, Norimasa; Doi, Shun'ichi

Disturbances decrease vehicle stability and increase driver's mental and physical workload. Especially unexpected disturbances such as lateral winds have severe effect on vehicle stability and driver's workload. This study aims at building a driver model of steering operations in lateral wind toward developing effective driver assistance system. First, the relationship between the driver's lateral motion and its reactive quick steering behavior is investigated using driving simulator with lateral 1dof motion. In the experiments, four different wind patterns are displayed by the simulator. As the results, strong correlation was found between the driver's head lateral jerk by the lateral disturbance and the angular acceleration of the steering wheel. Then, we build a mathematical model of driver's steering model from lateral disturbance input to steering torque of the reactive quick feed-forward steering based on the experimental results. Finally, validity of the proposed model is shown by comparing the steering torque of experimental results and that of simulation results.

Delivery of Cargo to Lysosomes Using GNeosomes.

Science.gov (United States)

Hamill, Kristina M; Wexselblatt, Ezequiel; Tong, Wenyong; Esko, Jeffrey D; Tor, Yitzhak

2017-01-01

Liposomes have been used to improve the intracellular delivery of a variety of cargos. Encapsulation of cargos in liposomes leads to improved plasma half-lives and minimized degradation. Here, we present a method for improving the selective delivery of liposomes to the lysosomes using a guanidinylated neomycin (GNeo) transporter. The method for synthesizing GNeo-lipids, incorporating them into liposomes, and the enhanced lysosomal delivery of encapsulated cargo are presented. GNeo-liposomes, termed GNeosomes, are capable of delivering a fluorescent dye to the lysosomes of Chinese hamster ovary cells as shown using confocal microscopy. GNeosomes can also be used to deliver therapeutic quantities of lysosomal enzymes to fibroblasts isolated from patients with a lysosomal storage disorder.

Steering, Entanglement, Nonlocality, and the EPR Paradox

OpenAIRE

Wiseman, H. M.; Jones, S. J.; Doherty, A. C.

2006-01-01

The concept of steering was introduced by Schrodinger in 1935 as a generalization of the EPR paradox for arbitrary pure bipartite entangled states and arbitrary measurements by one party. Until now, it has never been rigorously defined, so it has not been known (for example) what mixed states are steerable (that is, can be used to exhibit steering). We provide an operational definition, from which we prove (by considering Werner states and Isotropic states) that steerable states are a strict ...

A simplified tether model for molecular motor transporting cargo

International Nuclear Information System (INIS)

Fang-Zhen, Li; Li-Chun, Jiang

2010-01-01

Molecular motors are proteins or protein complexes which function as transporting engines in biological cells. This paper models the tether between motor and its cargo as a symmetric linear potential. Different from Elston and Peskin's work for which performance of the system was discussed only in some limiting cases, this study produces analytic solutions of the problem for general cases by simplifying the transport system into two physical states, which makes it possible to discuss the dynamics of the motor–cargo system in detail. It turns out that the tether strength between motor and cargo should be greater than a threshold or the motor will fail to transport the cargo, which was not discussed by former researchers yet. Value of the threshold depends on the diffusion coefficients of cargo and motor and also on the strength of the Brownian ratchets dragging the system. The threshold approaches a finite constant when the strength of the ratchet tends to infinity. (general)

Characteristics of future air cargo demand and impact on aircraft development: A report on the Cargo/Logistic Airlift Systems Study (CLASS) project

Science.gov (United States)

Whitehead, A. H., Jr.

1978-01-01

Current domestic and international air cargo operations are studied and the characteristics of 1990 air cargo demand are postulated from surveys conducted at airports and with shippers, consignees, and freight forwarders as well as air, land, and ocean carriers. Simulation and route optimization programs are exercised to evaluate advanced aircraft concepts. The results show that proposed changes in the infrastructure and improved cargo loading efficiencies are as important enhancing the prospects of air cargo growth as is the advent of advanced freighter aircraft. Potential reductions in aircraft direct operating costs are estimated and related to future total revenue. Service and cost elasticities are established and utilized to estimate future potential tariff reductions that may be realized through direct and indirect operating cost reductions and economies of scale.

77 FR 65006 - Air Cargo Advance Screening (ACAS) Pilot Program

Science.gov (United States)

2012-10-24

... electronic cargo information by way of a CBP-approved electronic data interchange (EDI) system before the... electronically receive certain information regarding that cargo through a CBP-approved EDI system no later than... the above information regarding that cargo through a CBP-approved EDI system no later than the time of...

User-driven sampling strategies in image exploitation

Science.gov (United States)

Harvey, Neal; Porter, Reid

2013-12-01

Visual analytics and interactive machine learning both try to leverage the complementary strengths of humans and machines to solve complex data exploitation tasks. These fields overlap most significantly when training is involved: the visualization or machine learning tool improves over time by exploiting observations of the human-computer interaction. This paper focuses on one aspect of the human-computer interaction that we call user-driven sampling strategies. Unlike relevance feedback and active learning sampling strategies, where the computer selects which data to label at each iteration, we investigate situations where the user selects which data is to be labeled at each iteration. User-driven sampling strategies can emerge in many visual analytics applications but they have not been fully developed in machine learning. User-driven sampling strategies suggest new theoretical and practical research questions for both visualization science and machine learning. In this paper we identify and quantify the potential benefits of these strategies in a practical image analysis application. We find user-driven sampling strategies can sometimes provide significant performance gains by steering tools towards local minima that have lower error than tools trained with all of the data. In preliminary experiments we find these performance gains are particularly pronounced when the user is experienced with the tool and application domain.

46 CFR 153.491 - Waiver of certain equipment for dedicated cargo tanks.

Science.gov (United States)

2010-10-01

... and endorses a ship's Certificate of Inspection or Certificate of Compliance allowing a cargo tank to carry a single, specific NLS cargo and no other cargo if the ship's owner— (1) Requests a waiver... DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design...

STEER Coastal Use Mapping Project

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Coastal Use Mapping Project is designed to collect critical information on human activities in and near the St. Thomas East End Reserves (STEER). The project...

Design of a Path-Tracking Steering Controller for Autonomous Vehicles

Directory of Open Access Journals (Sweden)

Chuanyang Sun

2018-06-01

Full Text Available This paper presents a linearization method for the vehicle and tire models under the model predictive control (MPC scheme, and proposes a linear model-based MPC path-tracking steering controller for autonomous vehicles. The steering controller is designed to minimize lateral path-tracking deviation at high speeds. The vehicle model is linearized by a sequence of supposed steering angles, which are obtained by assuming the vehicle can reach the desired path at the end of the MPC prediction horizon and stay in a steady-state condition. The lateral force of the front tire is directly used as the control input of the model, and the rear tire’s lateral force is linearized by an equivalent cornering stiffness. The course-direction deviation, which is the angle between the velocity vector and the path heading, is chosen as a control reference state. The linearization model is validated through the simulation, and the results show high prediction accuracy even in regions of large steering angle. This steering controller is tested through simulations on the CarSim-Simulink platform (R2013b, MathWorks, Natick, MA, USA, showing the improved performance of the present controller at high speeds.

The Impact of Analog and Bang-Bang Steering Gear Control on Ship's Fuel Economy

DEFF Research Database (Denmark)

Nørtoft Thomsen, J. C.; Blanke, Mogens; Reid, R. E.

1982-01-01

it is found to be at least equally important regarding steering performance and fuel economy. The paper presents a comprehensive survey of steering gear principles commonly used, including relevant details of three analog steering gear servo principles, which have outperformed conventional designs. Control......The latest years have shown considerable efforts towards improving steering generated propulsion losses of ships by the introduction of various sophisticated control algorithms in the autopilots. However, little previous attention has been given to the steering gear control loop, although...

Transport of the moving barrier driven by chiral active particles

Science.gov (United States)

Liao, Jing-jing; Huang, Xiao-qun; Ai, Bao-quan

2018-03-01

Transport of a moving V-shaped barrier exposed to a bath of chiral active particles is investigated in a two-dimensional channel. Due to the chirality of active particles and the transversal asymmetry of the barrier position, active particles can power and steer the directed transport of the barrier in the longitudinal direction. The transport of the barrier is determined by the chirality of active particles. The moving barrier and active particles move in the opposite directions. The average velocity of the barrier is much larger than that of active particles. There exist optimal parameters (the chirality, the self-propulsion speed, the packing fraction, and the channel width) at which the average velocity of the barrier takes its maximal value. In particular, tailoring the geometry of the barrier and the active concentration provides novel strategies to control the transport properties of micro-objects or cargoes in an active medium.

Violation of Continuous-Variable Einstein-Podolsky-Rosen Steering with Discrete Measurements

Science.gov (United States)

Schneeloch, James; Dixon, P. Ben; Howland, Gregory A.; Broadbent, Curtis J.; Howell, John C.

2013-03-01

In this Letter, we derive an entropic Einstein-Podolsky-Rosen (EPR) steering inequality for continuous-variable systems using only experimentally measured discrete probability distributions and details of the measurement apparatus. We use this inequality to witness EPR steering between the positions and momenta of photon pairs generated in spontaneous parametric down-conversion. We examine the asymmetry between parties in this inequality, and show that this asymmetry can be used to reduce the technical requirements of experimental setups intended to demonstrate the EPR paradox. Furthermore, we develop a more stringent steering inequality that is symmetric between parties, and use it to show that the down-converted photon pairs also exhibit symmetric EPR steering.

The Front Steering Launcher Design for the ITER ECRH Upper Port

International Nuclear Information System (INIS)

Henderson, M A; Chavan, R; Heidinger, R; Nikkola, P; Ramponi, G; Saibene, G; Sanchez, F; Sauter, O; Serikov, A; Zohm, H

2005-01-01

The ECRH ITER upper port antenna's role is to stabilize the neoclassical tearing mode (NTM) on either the q = 2 or 3/2 rational flux surfaces, which requires a narrow current deposition profile (j CD ) over a wide range along the resonance surfaces. The width of j CD should be equivalent to the marginal island width to fully stabilise the NTM. Two antenna concepts are under consideration for the upper port launcher: front steering (FS) and remote steering (RS). The FS launcher decouples the steering and focusing aspects using a two mirror system (one focusing and one steering), achieving a wider steering range and higher current density for NTM stabilisation than required by ITER, offering a threefold increase in NTM stabilization efficiency over the RS launcher. The improved physics performance has motivated the further design study of the FS launcher aiming toward a build to print launcher. The present design is compatible with ≥2.0 MW CW operation and 8 beams per port plug. A frictionless backlashfree system is envisioned for the steering mechanism. An overview of the launcher design, the calculated physics performance and the possibility of using the upper port launcher for extended physics applications (beyond NTM stabilisation) are discussed

Feed efficiency differences and reranking in beef steers fed grower and finisher diets.

Science.gov (United States)

Durunna, O N; Mujibi, F D N; Goonewardene, L; Okine, E K; Basarab, J A; Wang, Z; Moore, S S

2011-01-01

This 3-yr study used 490 steers to determine whether feedlot steers changed their feed efficiency (FE) ranking when fed a grower diet, then a finisher diet. The steers were crossbreds and were between 5 to 7 mo of age. There were 2 feeding periods each year. Within each year, approximately 90 steers had their diet switched from a grower to a finisher diet (feed-swap group), whereas another 90 steers were fed either the grower (grower-fed group) or the finisher (finisher-fed group) diet throughout the feeding trial. Each feeding test lasted for a minimum of 10 wk, and all steers were fed ad libitum. Individual animal feed intakes were collected using the GrowSafe feeding system, and BW were measured every 2 wk. Residual feed intake (RFI), G:F, and Kleiber ratio (KR) were computed at the end of each feeding period. For each measure of efficiency, animals were classified as low, medium, or high based on 0.5 SD from the mean. The majority of steers did not maintain the previous efficiency class in the second period. Approximately 58, 51, and 51% of steers in the feed-swap group, finisher-fed group, and the grower-fed group, respectively, changed their RFI measure by 0.5 SD. A low rank correlation occurred in all test groups but was less in the feed-swap group. Spearman rank correlations between the 2 feeding periods in the feed-swap group were 0.33, 0.20, and 0.31 for RFI, G:F, and KR, respectively. Classifications based on G:F and KR showed that a greater number of steers (P 0.05) between the proportions of individuals that changed or maintained their FE class. In the groups without a feed-swap, there was no difference (P > 0.05) in the proportion of steers that changed or maintained the same FE class for all FE measures. Our results suggest that diet type and feeding period affect the FE ranking in beef steers. A feedlot diet is ideal for evaluating the FE potential of steers for feedlot profitability; however, we suggest that tests involving less dense diets should

The mechanochemical cycle of mammalian kinesin-2 KIF3A/B under load

Science.gov (United States)

Andreasson, Johan O.L.; Shastry, Shankar; Hancock, William O.; Block, Steven M.

2015-01-01

Summary The response of motor proteins to external loads underlies their ability to work in teams and determines the net speed and directionality of cargo transport. The mammalian kinesin-2, KIF3A/B, is a heterotrimeric motor involved in intraflagellar transport and vesicle motility in neurons. Bidirectional cargo transport is known to result from the opposing activities of KIF3A/B and dynein bound to the same cargo, but the load-dependent properties of kinesin-2 are poorly understood. We used a feedback-controlled optical trap to probe the velocity, run length and unbinding kinetics of mouse KIF3A/B under various loads and nucleotide conditions. The kinesin-2 motor velocity is less sensitive than kinesin-1 to external forces, but its processivity diminishes steeply with load, and the motor was observed occasionally to slip and reattach. Each motor domain was characterized by studying homodimeric constructs, and a global fit to the data resulted in a comprehensive pathway that quantifies the principal force-dependent kinetic transitions. The properties of the KIF3A/B heterodimer are intermediate between the two homodimers, and the distinct load-dependent behavior is attributable to the properties of the motor domains, and not to the neck-linkers or the coiled-coil stalk. We conclude that the force-dependent movement of KIF3A/B differs significantly from conventional kinesin-1. Against opposing dynein forces, KIF3A/B motors are predicted to rapidly unbind and rebind, resulting in qualitatively different transport behavior from kinesin-1. PMID:25866395

Distinct cargo-specific response landscapes underpin the complex and nuanced role of galectin-glycan interactions in clathrin-independent endocytosis.

Science.gov (United States)

Mathew, Mohit P; Donaldson, Julie G

2018-05-11

Clathrin-independent endocytosis (CIE) is a form of endocytosis that lacks a defined cytoplasmic machinery. Here, we asked whether glycan interactions, acting from the outside, could be a part of that endocytic machinery. We show that the perturbation of global cellular patterns of protein glycosylation by modulation of metabolic flux affects CIE. Interestingly, these changes in glycosylation had cargo-specific effects. For example, in HeLa cells, GlcNAc treatment, which increases glycan branching, increased major histocompatibility complex class I (MHCI) internalization but inhibited CIE of the glycoprotein CD59 molecule (CD59). The effects of knocking down the expression of galectin 3, a carbohydrate-binding protein and an important player in galectin-glycan interactions, were also cargo-specific and stimulated CD59 uptake. By contrast, inhibition of all galectin-glycan interactions by lactose inhibited CIE of both MHCI and CD59. None of these treatments affected clathrin-mediated endocytosis, implying that glycosylation changes specifically affect CIE. We also found that the galectin lattice tailors membrane fluidity and cell spreading. Furthermore, changes in membrane dynamics mediated by the galectin lattice affected macropinocytosis, an altered form of CIE, in HT1080 cells. Our results suggest that glycans play an important and nuanced role in CIE, with each cargo being affected uniquely by alterations in galectin and glycan profiles and their interactions. We conclude that galectin-driven effects exist on a continuum from stimulatory to inhibitory, with distinct CIE cargo proteins having unique response landscapes and with different cell types starting at different positions on these conceptual landscapes.

Influence of orographically steered winds on Mutsu Bay surface currents

Science.gov (United States)

Yamaguchi, Satoshi; Kawamura, Hiroshi

2005-09-01

Effects of spatially dependent sea surface wind field on currents in Mutsu Bay, which is located at the northern end of Japanese Honshu Island, are investigated using winds derived from synthetic aperture radar (SAR) images and a numerical model. A characteristic wind pattern over the bay was evidenced from analysis of 118 SAR images and coincided with in situ observations. Wind is topographically steered with easterly winds entering the bay through the terrestrial gap and stronger wind blowing over the central water toward its mouth. Nearshore winds are weaker due to terrestrial blockages. Using the Princeton Ocean Model, we investigated currents forced by the observed spatially dependent wind field. The predicted current pattern agrees well with available observations. For a uniform wind field of equal magnitude and average direction, the circulation pattern departs from observations demonstrating that vorticity input due to spatially dependent wind stress is essential in generation of the wind-driven current in Mutsu Bay.

Dynein-Based Accumulation of Membranes Regulates Nuclear Expansion in Xenopus laevis Egg Extracts.

Science.gov (United States)

Hara, Yuki; Merten, Christoph A

2015-06-08

Nuclear size changes dynamically during development and has long been observed to correlate with the space surrounding the nucleus, as well as with the volume of the cell. Here we combine an in vitro cell-free system of Xenopus laevis egg extract with microfluidic devices to systematically analyze the effect of spatial constraints. The speed of nuclear expansion depended on the available space surrounding the nucleus up to a threshold volume in the nanoliter range, herein referred to as the nuclear domain. Under spatial constraints smaller than this nuclear domain, the size of microtubule-occupied space surrounding the nucleus turned out to be limiting for the accumulation of membranes around the nucleus via the motor protein dynein, therefore determining the speed of nuclear expansion. This mechanism explains how spatial information surrounding the nucleus, such as the positioning of the nucleus inside the cell, can control nuclear expansion. Copyright © 2015 Elsevier Inc. All rights reserved.

Data-driven automatic parking constrained control for four-wheeled mobile vehicles

OpenAIRE

Wenxu Yan; Jing Deng; Dezhi Xu

2016-01-01

In this article, a novel data-driven constrained control scheme is proposed for automatic parking systems. The design of the proposed scheme only depends on the steering angle and the orientation angle of the car, and it does not involve any model information of the car. Therefore, the proposed scheme-based automatic parking system is applicable to different kinds of cars. In order to further reduce the desired trajectory coordinate tracking errors, a coordinates compensation algorithm is als...

The Highly Conserved COPII Coat Complex Sorts Cargo from the Endoplasmic Reticulum and Targets It to the Golgi

OpenAIRE

Lord, Christopher; Ferro-Novick, Susan; Miller, Elizabeth A.

2013-01-01

Protein egress from the endoplasmic reticulum (ER) is driven by a conserved cytoplasmic coat complex called the COPII coat. The COPII coat complex contains an inner shell (Sec23/Sec24) that sorts cargo into ER-derived vesicles and an outer cage (Sec13/Sec31) that leads to coat polymerization. Once released from the ER, vesicles must tether to and fuse with the target membrane to deliver their protein and lipid contents. This delivery step also depends on the COPII coat, with coat proteins bin...

Test of Einstein-Podolsky-Rosen steering based on the all-versus-nothing proof.

Science.gov (United States)

Wu, Chunfeng; Chen, Jing-Ling; Ye, Xiang-Jun; Su, Hong-Yi; Deng, Dong-Ling; Wang, Zhenghan; Oh, C H

2014-03-06

In comparison with entanglement and Bell nonlocality, Einstein-Podolsky-Rosen steering is a newly emerged research topic and in its incipient stage. Although Einstein-Podolsky-Rosen steering has been explored via violations of steering inequalities both theoretically and experimentally, the known inequalities in the literatures are far from well-developed. As a result, it is not yet possible to observe Einstein-Podolsky-Rosen steering for some steerable mixed states. Recently, a simple approach was presented to identify Einstein-Podolsky-Rosen steering based on all-versus-nothing argument, offering a strong condition to witness the steerability of a family of two-qubit (pure or mixed) entangled states. In this work, we show that the all-versus-nothing proof of Einstein-Podolsky-Rosen steering can be tested by measuring the projective probabilities. Through the bound of probabilities imposed by local-hidden-state model, the proposed test shows that steering can be detected by the all-versus-nothing argument experimentally even in the presence of imprecision and errors. Our test can be implemented in many physical systems and we discuss the possible realizations of our scheme with non-Abelian anyons and trapped ions.

The Design of Bulk Carrier Cargo Holds State Integrated Monitoring System

Directory of Open Access Journals (Sweden)

Gao Ru-jiang

2016-01-01

Full Text Available Most ship cargo hold Internal uses artificial watch or is unattended. Therefore, it is impossible to know the appropriate information of the cargo holds Internal timely and accurately. Cargo damage and ship accidents occurred frequently. Automation remote processing and monitoring alarm system for the bulk carrier is an important part of the marine automation. The system plays a significant role to guarantee the navigation safety for bulk carriers. The paper introduced the important parts of the integrated monitoring system, structural design, hardware configuration, various modules communication transmission and various data processing software design were included. Based on embedded development, the real time information including the cargo hold internal humidity temperature, oxygen concentration, smoke gas concentration, cold water well level and other data were collected, as well the hatch closed state was detected and the cargo hold internal real time video information was collected. Then the real-time communication between the control display and acquisition modules were assured. By adjusting the corresponding buttons on the bridge according to the monitoring information, so that the cargo hold always in a safe environment, so as to avoid cargo hold accidents.

Novel Microstrip Patch Antennas with Frequency Agility, Polarization Reconfigurability, Dual Null Steering Capability and Phased Array Antenna with Beam Steering Performance

Science.gov (United States)

Babakhani, Behrouz

Nowadays the wireless communication technology is playing an important role in our daily life. People use wireless devices not only as a conventional communication device but also as tracking and navigation tool, web browsing tool, data storage and transfer tool and so for many other reasons. Based on the user demand, wireless communication engineers try to accommodate as many as possible wireless systems and applications in a single device and therefore, creates a multifunctional device. Antenna, as an integral part of any wireless communication systems, should also be evolved and adjusted with development of wireless transceiver systems. Therefore multifunctional antennas have been introduced to support and enhance the functionality on modern wireless systems. The main focus and contribution of this thesis is design of novel multifunctional microstrip antennas with frequency agility, polarization reconfigurablity, dual null steering capability and phased array antenna with beam steering performance. In this thesis, first, a wide bandwidth(1.10 GHz to 1.60 GHz) right-handed circularly polarized (RHCP) directional antenna for global positioning system (GPS) satellite receive application has been introduced which covers all the GPS bands starting from L1 to L5. This design consists of two crossed bow-tie dipole antennas fed with sequentially phase rotated feed network backed with an artificial high impedance surface (HIS) structure to generate high gain directional radiation patterns. This design shows good CP gain and axial ratio (AR) and wide beamwidth performance. Although this design has good radiation quality, the size and the weight can be reduced as future study. In the second design, a frequency agile antenna was developed which also covers the L-band (L1 to L5) satellite communication frequencies. This frequency agile antenna was designed and realized by new implementation of varactor diodes in the geometry of a circular patch antenna. Beside wide frequency

19 CFR 4.62 - Accounting for inward cargo.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 1 2010-04-01 2010-04-01 false Accounting for inward cargo. 4.62 Section 4.62 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY VESSELS IN FOREIGN AND DOMESTIC TRADES Foreign Clearances § 4.62 Accounting for inward cargo...

Comparative International Air Cargo Solutions: The Pathway to a Resilient, Adaptable, Balanced and Sustainable Secure Global Air Cargo Supply Chain

Science.gov (United States)

2013-12-01

counterfeit merchandise smugglers, and remains the weakest link in the air cargo transportation chain. The TSA and CBP continue to seek administrative...been exploited by narcotics, human and counterfeit merchandise smugglers for centuries, remains the weakest link in the air cargo transportation chain... visual depiction of the European Union 3rd country security processes. 96 Official Journal of the

Design of Model-based Controller with Disturbance Estimation in Steer-by-wire System

Directory of Open Access Journals (Sweden)

Jung Sanghun

2018-01-01

Full Text Available The steer-by-wire system is a next generation steering control technology that has been actively studied because it has many advantages such as fast response, space efficiency due to removal of redundant mechanical elements, and high connectivity with vehicle chassis control, such as active steering. Steer-by-wire system has disturbance composed of tire friction torque and self-aligning torque. These disturbances vary widely due to the weight or friction coefficient change. Therefore, disturbance compensation logic is strongly required to obtain desired performance. This paper proposes model-based controller with disturbance compensation to achieve the robust control performance. Targeted steer-by-wire system is identified through the experiment and system identification method. Moreover, model-based controller is designed using the identified plant model. Disturbance of targeted steer-by-wire is estimated using disturbance observer(DOB, and compensate the estimated disturbance into control input. Experiment of various scenarios are conducted to validate the robust performance of proposed model-based controller.

On Optimizing Steering Performance of Multi-axle Vehicle Based on Driving Force Control

Directory of Open Access Journals (Sweden)

Wu Zhicheng

2017-01-01

Full Text Available The steering performance of multi-axle vehicle with independent driving system is affected by the distribution of the wheel driving force. A nonlinear vehicle dynamics model including magic formula tire model for describing 11 DoF four-axle vehicle with dual-front-axle-steering (DFAS system was presented. The influence of different driving force distribution scheme on the steering performance of the vehicle was analyzed. A control strategy for improving the steady response and transient response of the vehicle steering is proposed. The results show: For the steady response, setting different drive force for internal and external wheels according to the actual steering characteristics of the vehicle can effectively improve its steering characteristics; For the transient response, adopting the zero sideslip angle control strategy and using the PID control algorithm to control the driving force of the outside wheel of tear-two-axle, under angle step input, the vehicle sideslip angle can quickly stabilize to 0 and yaw rate also significantly decreases.

A Vehicle Steering Recognition System Based on Low-Cost Smartphone Sensors

Directory of Open Access Journals (Sweden)

Xinhua Liu

2017-03-01

Full Text Available Recognizing how a vehicle is steered and then alerting drivers in real time is of utmost importance to the vehicle and driver’s safety, since fatal accidents are often caused by dangerous vehicle maneuvers, such as rapid turns, fast lane-changes, etc. Existing solutions using video or in-vehicle sensors have been employed to identify dangerous vehicle maneuvers, but these methods are subject to the effects of the environmental elements or the hardware is very costly. In the mobile computing era, smartphones have become key tools to develop innovative mobile context-aware systems. In this paper, we present a recognition system for dangerous vehicle steering based on the low-cost sensors found in a smartphone: i.e., the gyroscope and the accelerometer. To identify vehicle steering maneuvers, we focus on the vehicle’s angular velocity, which is characterized by gyroscope data from a smartphone mounted in the vehicle. Three steering maneuvers including turns, lane-changes and U-turns are defined, and a vehicle angular velocity matching algorithm based on Fast Dynamic Time Warping (FastDTW is adopted to recognize the vehicle steering. The results of extensive experiments show that the average accuracy rate of the presented recognition reaches 95%, which implies that the proposed smartphone-based method is suitable for recognizing dangerous vehicle steering maneuvers.

Cargo-mix optimization in Liner Shipping

DEFF Research Database (Denmark)

Christensen, Jonas Mark; Pacino, Dario; Fonseca, Joao Filipe Paiva

International transportation constitutes one of the biggest challenges in limiting CO2 emission in theworld: it is technically hard to find viable alternatives to fossil fuels, and due to the internationalnature, it is very difficult to regulate CO2 emission of intercontinental trade. Moreover...... and have to find a load configuration (stowage plan)that both suits the current cargo to be loaded but also guarantees that the vessel can be utilizedto its maximum in future ports. The size of nowadays vessels is, however, making this work harderand harder (Pacino et al. (2011)). Moreover, the cargo...

Demonstration of Einstein-Podolsky-Rosen steering with enhanced subchannel discrimination

Science.gov (United States)

Sun, Kai; Ye, Xiang-Jun; Xiao, Ya; Xu, Xiao-Ye; Wu, Yu-Chun; Xu, Jin-Shi; Chen, Jing-Ling; Li, Chuan-Feng; Guo, Guang-Can

2018-03-01

Einstein-Podolsky-Rosen (EPR) steering describes a quantum nonlocal phenomenon in which one party can nonlocally affect the other's state through local measurements. It reveals an additional concept of quantum non-locality, which stands between quantum entanglement and Bell nonlocality. Recently, a quantum information task named as subchannel discrimination (SD) provides a necessary and sufficient characterization of EPR steering. The success probability of SD using steerable states is higher than using any unsteerable states, even when they are entangled. However, the detailed construction of such subchannels and the experimental realization of the corresponding task are still technologically challenging. In this work, we designed a feasible collection of subchannels for a quantum channel and experimentally demonstrated the corresponding SD task where the probabilities of correct discrimination are clearly enhanced by exploiting steerable states. Our results provide a concrete example to operationally demonstrate EPR steering and shine a new light on the potential application of EPR steering.

Stochastic stability of four-wheel-steering system

International Nuclear Information System (INIS)

Huang Dongwei; Wang Hongli; Zhu Zhiwen; Feng Zhang

2007-01-01

A four-wheel-steering system subjected to white noise excitations was reduced to a two-degree-of-freedom quasi-non-integrable-Hamiltonian system. Subsequently we obtained an one-dimensional Ito stochastic differential equation for the averaged Hamiltonian of the system by using the stochastic averaging method for quasi-non-integrable-Hamiltonian systems. Thus, the stochastic stability of four-wheel-steering system was analyzed by analyzing the sample behaviors of the averaged Hamiltonian at the boundary H = 0 and calculating its Lyapunov exponent. An example given at the end demonstrated that the conclusion obtained is of considerable significance

49 CFR 173.33 - Hazardous materials in cargo tank motor vehicles.

Science.gov (United States)

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Hazardous materials in cargo tank motor vehicles... Transportation § 173.33 Hazardous materials in cargo tank motor vehicles. (a) General requirements. (1) No person may offer or accept a hazardous material for transportation in a cargo tank motor vehicle except as...

Ultra-compact laser beam steering device using holographically formed two dimensional photonic crystal.

Science.gov (United States)

Dou, Xinyuan; Chen, Xiaonan; Chen, Maggie Yihong; Wang, Alan Xiaolong; Jiang, Wei; Chen, Ray T

2010-03-01

In this paper, we report the theoretical study of polymer-based photonic crystals for laser beam steering which is based on the superprism effect as well as the experiment fabrication of the two dimensional photonic crystals for the laser beam steering. Superprism effect, the principle for beam steering, was separately studied in details through EFC (Equifrequency Contour) analysis. Polymer based photonic crystals were fabricated through double exposure holographic interference method using SU8-2007. The experiment results showed a beam steering angle of 10 degree for 30 nm wavelength variation.

Safety of Oversize Cargo in Ports and in the Sea Transport

Directory of Open Access Journals (Sweden)

Miroslaw Chmielinski

2017-03-01

Full Text Available Author of the paper analyzes problems related to the safety of the oversize cargo in ports and in the sea transport. Various modes of transport are used to carry oversized units that often include maritime transport. Transport of oversized cargo includes non-standard large and heavy pieces of cargo, such as electric transformers, reactor vessels, wind turbines, airplane fuselage or nuclear power plant components. The above paper is based on results of research oversized cargo in the Elpo Service Company and Pol-Mare Ltd. forwarding consulting agency.

Loophole-free Einstein-Podolsky-Rosen experiment via quantum steering

International Nuclear Information System (INIS)

Wittmann, Bernhard; Ramelow, Sven; Zeilinger, Anton; Steinlechner, Fabian; Langford, Nathan K; Ursin, Rupert; Brunner, Nicolas; Wiseman, Howard M

2012-01-01

Tests of the predictions of quantum mechanics for entangled systems have provided increasing evidence against local realistic theories. However, there remains the crucial challenge of simultaneously closing all major loopholes—the locality, freedom-of-choice and detection loopholes—in a single experiment. An important sub-class of local realistic theories can be tested with the concept of ‘steering’. The term ‘steering’ was introduced by Schrödinger in 1935 for the fact that entanglement would seem to allow an experimenter to remotely steer the state of a distant system as in the Einstein-Podolsky-Rosen (EPR) argument. Einstein called this ‘spooky action at a distance’. EPR-steering has recently been rigorously formulated as a quantum information task opening it up to new experimental tests. Here, we present the first loophole-free demonstration of EPR-steering by violating three-setting quadratic steering inequality, tested with polarization-entangled photons shared between two distant laboratories. Our experiment demonstrates this effect while simultaneously closing all loopholes: both the locality loophole and a specific form of the freedom-of-choice loophole are closed by having a large separation of the parties and using fast quantum random number generators, and the fair-sampling loophole is closed by having high overall detection efficiency. Thereby, we exclude—for the first time loophole-free—an important class of local realistic theories considered by EPR. Besides its foundational importance, loophole-free steering also allows the distribution of quantum entanglement secure event in the presence of an untrusted party. (paper)

Loophole-free Einstein-Podolsky-Rosen experiment via quantum steering

Science.gov (United States)

Wittmann, Bernhard; Ramelow, Sven; Steinlechner, Fabian; Langford, Nathan K.; Brunner, Nicolas; Wiseman, Howard M.; Ursin, Rupert; Zeilinger, Anton

2012-05-01

Tests of the predictions of quantum mechanics for entangled systems have provided increasing evidence against local realistic theories. However, there remains the crucial challenge of simultaneously closing all major loopholes—the locality, freedom-of-choice and detection loopholes—in a single experiment. An important sub-class of local realistic theories can be tested with the concept of ‘steering’. The term ‘steering’ was introduced by Schrödinger in 1935 for the fact that entanglement would seem to allow an experimenter to remotely steer the state of a distant system as in the Einstein-Podolsky-Rosen (EPR) argument. Einstein called this ‘spooky action at a distance’. EPR-steering has recently been rigorously formulated as a quantum information task opening it up to new experimental tests. Here, we present the first loophole-free demonstration of EPR-steering by violating three-setting quadratic steering inequality, tested with polarization-entangled photons shared between two distant laboratories. Our experiment demonstrates this effect while simultaneously closing all loopholes: both the locality loophole and a specific form of the freedom-of-choice loophole are closed by having a large separation of the parties and using fast quantum random number generators, and the fair-sampling loophole is closed by having high overall detection efficiency. Thereby, we exclude—for the first time loophole-free—an important class of local realistic theories considered by EPR. Besides its foundational importance, loophole-free steering also allows the distribution of quantum entanglement secure event in the presence of an untrusted party.

Cooperating to Compete in the Global Air Cargo Industry: The Case of the DHL Express and Lufthansa Cargo A.G. Joint Venture Airline ‘AeroLogic’

Directory of Open Access Journals (Sweden)

Glenn Baxter

2018-03-01

Full Text Available This paper presents a case study of the DHL Express and Lufthansa Cargo strategic joint venture cargo airline ‘AeroLogic’, the global air cargo industry’s largest operative joint venture between an airline and a leading international express and logistics provider. The study used a qualitative research approach. The data gathered for the study was examined by document analysis. The strategic analysis of the AeroLogic joint venture was based on the use of Porter’s Five Forces framework. The study found that the AeroLogic joint venture airline has provided synergistic benefits to both partners and has allowed the partners to access new markets and to participate in the evolution of the air cargo industry. The new venture has also enabled both joint venture partners to enhance their competitive position in the global air cargo industry through strengthened service offerings and has provided the partners with increased cargo capacities, a larger route network, and greater frequencies within their own route networks. The study also found that the AeroLogic business model is unique in the air cargo industry. A limitation of the study was that AeroLogic’s annual revenue or freight traffic data was not available. It was, therefore, not possible to analyse the business performance of the joint venture.

Final Report, Next-Generation Mega-Voltage Cargo-Imaging System for Cargo Conainer Inspection, March 2007

Energy Technology Data Exchange (ETDEWEB)

Dr. James Clayton, Ph.D., Varian Medical Systems-Security & Inspection Products; Dr. Emma Regentova, Ph.D, University of Nevada Las Vegas; Dr. Evangelos Yfantis, Ph.D., University of Nevada, Las Vegas

2007-03-27

The UNLV Research Foundation, as the primary award recipient, teamed with Varian Medical Systems-Security & Inspection Products and the University of Nevada Las Vegas (UNLV) for the purpose of conducting research and engineering related to a "next-generation" mega-voltage imaging (MVCI) system for inspection of cargo in large containers. The procurement and build-out of hardware for the MVCI project has been completed. The K-9 linear accelerator and an optimized X-ray detection system capable of efficiently detecting X-rays emitted from the accelerator after they have passed through the device is under test. The Office of Science financial assistance award has made possible the development of a system utilizing a technology which will have a profound positive impact on the security of U.S. seaports. The proposed project will ultimately result in critical research and development advances for the "next-generation" Linatron X-ray accelerator technology, thereby providing a safe, reliable and efficient fixed and mobile cargo inspection system, which will very significantly increase the fraction of cargo containers undergoing reliable inspection as the enter U.S. ports. Both NNSA/NA-22 and the Department of Homeland Security's Domestic Nuclear Detection Office are collaborating with UNLV and its team to make this technology available as soon as possible.

Final Report, Next-Generation Mega-Voltage Cargo-Imaging System for Cargo Container Inspection, March 2007

International Nuclear Information System (INIS)

Dr. James Clayton, Ph.D., Varian Medical Systems-Security and Inspection Products; Dr. Emma Regentova, Ph.D, University of Nevada Las Vegas; Dr. Evangelos Yfantis, Ph.D., University of Nevada, Las Vegas

2007-01-01

The UNLV Research Foundation, as the primary award recipient, teamed with Varian Medical Systems-Security and Inspection Products and the University of Nevada Las Vegas (UNLV) for the purpose of conducting research and engineering related to a ''next-generation'' mega-voltage imaging (MVCI) system for inspection of cargo in large containers. The procurement and build-out of hardware for the MVCI project has been completed. The K-9 linear accelerator and an optimized X-ray detection system capable of efficiently detecting X-rays emitted from the accelerator after they have passed through the device is under test. The Office of Science financial assistance award has made possible the development of a system utilizing a technology which will have a profound positive impact on the security of U.S. seaports. The proposed project will ultimately result in critical research and development advances for the ''next-generation'' Linatron X-ray accelerator technology, thereby providing a safe, reliable and efficient fixed and mobile cargo inspection system, which will very significantly increase the fraction of cargo containers undergoing reliable inspection as the enter U.S. ports. Both NNSA/NA-22 and the Department of Homeland Security's Domestic Nuclear Detection Office are collaborating with UNLV and its team to make this technology available as soon as possible

46 CFR 153.976 - Transfer of packaged cargo or ship's stores.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Transfer of packaged cargo or ship's stores. 153.976 Section 153.976 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Operations Cargo Transfer Procedures § 153.976 Transfer of...

46 CFR 154.650 - Cargo tank and process pressure vessel welding.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Cargo tank and process pressure vessel welding. 154.650... Equipment Construction § 154.650 Cargo tank and process pressure vessel welding. (a) Cargo tank and process pressure vessel welding must meet Subpart 54.05 and Part 57 of this chapter. (b) Welding consumables used...

Dissipation induced asymmetric steering of distant atomic ensembles

Science.gov (United States)

Cheng, Guangling; Tan, Huatang; Chen, Aixi

2018-04-01

The asymmetric steering effects of separated atomic ensembles denoted by the effective bosonic modes have been explored by the means of quantum reservoir engineering in the setting of the cascaded cavities, in each of which an atomic ensemble is involved. It is shown that the steady-state asymmetric steering of the mesoscopic objects is unconditionally achieved via the dissipation of the cavities, by which the nonlocal interaction occurs between two atomic ensembles, and the direction of steering could be easily controlled through variation of certain tunable system parameters. One advantage of the present scheme is that it could be rather robust against parameter fluctuations, and does not require the accurate control of evolution time and the original state of the system. Furthermore, the double-channel Raman transitions between the long-lived atomic ground states are used and the atomic ensembles act as the quantum network nodes, which makes our scheme insensitive to the collective spontaneous emission of atoms.

Einstein-Podolsky-Rosen-steering swapping between two Gaussian multipartite entangled states

Science.gov (United States)

Wang, Meihong; Qin, Zhongzhong; Wang, Yu; Su, Xiaolong

2017-08-01

Multipartite Einstein-Podolsky-Rosen (EPR) steering is a useful quantum resource for quantum communication in quantum networks. It has potential applications in secure quantum communication, such as one-sided device-independent quantum key distribution and quantum secret sharing. By distributing optical modes of a multipartite entangled state to space-separated quantum nodes, a local quantum network can be established. Based on the existing multipartite EPR steering in a local quantum network, secure quantum communication protocol can be accomplished. In this manuscript, we present swapping schemes for EPR steering between two space-separated Gaussian multipartite entangled states, which can be used to connect two space-separated quantum networks. Two swapping schemes, including the swapping between a tripartite Greenberger-Horne-Zeilinger (GHZ) entangled state and an EPR entangled state and that between two tripartite GHZ entangled states, are analyzed. Various types of EPR steering are presented after the swapping of two space-separated independent multipartite entanglement states without direct interaction, which can be used to implement quantum communication between two quantum networks. The presented schemes provide technical reference for more complicated quantum networks with EPR steering.

Sources of carbohydrates in the ingestive behavior of feedlot steers

Directory of Open Access Journals (Sweden)

Viviane Santos da Silva

2014-05-01

Full Text Available In this article we research the influence of different sources of carbohydrates (corn, soybean hulls or wheat bran upon the digestive behavior of 24 confined castrated steers with an initial average age and weight of 20 months and 330 kg born from the cross between Charolais and Nellore. The diet was composed of 40% sorghum silage and 60% concentrate. The time spent on total ruminating (an average of 454.6 min/day was not influenced by the source of carbohydrate. The animals from the wheat bran treatment spent less time idle (718 min in relation to those on the corn and soybean hulls treatments, which did not differ between themselves (an average of 792 min/day. The steers from the wheat bran treatment remained less time feeding (184 min/day compared with those fed the other treatments, whose average time of permanence in this activity was 214 minutes per day. The other studied variables did not present a significant difference between the treatments. Inclusion of wheat bran in the diet of the confined steers results in less spent time idle, while steers feeding on soybean hulls spend less time feeding. The use of corn, soybean hulls, or wheat bran in the diet of the confined steers does not affect the total cudding time.

Beam steering for circular switched parasitic arrays using a combinational approach

CSIR Research Space (South Africa)

Mofolo, ROM

2011-09-01

Full Text Available In this paper, the authors present a method of electronic beam steering for circular switched parasitic array (SPA) antennas. In circular SPA antennas, one achieves azimuth beam steering by open-circuiting and short-circuiting different parasitic...

comparative beef production from buli,s, steers and heifers under

African Journals Online (AJOL)

steers, although statistically significant, were much srnaller than differences obtained in the present study. Out of a maximum possible grading of 20 points, bulls obtained an average of 12,3 points (Grade I -) which is considerably lower than the grading points obtained by steers (16,3 = Prime) or by heifers (17,5 = Prime+).

Design, Modeling And Control Of Steering And Braking For An Urban Electric Vehicle

OpenAIRE

Maciua, Dragos

1996-01-01

This report describes research which involved the design modification, modeling and control of automatic steering and braking systems for an urban electric vehicle. The vehicle is equipped with four-wheel independent drive, four-wheel independent braking and four-wheel steering. Control algorithms were developed for steering and braking. Simulation results show the feasibility of the algorithms.

46 CFR 42.25-15 - Stowage.

Science.gov (United States)

2010-10-01

... way with the navigation and necessary work of the vessel. (b) Upright. (1) Uprights, when required by... flexible wire rope of equivalent strength, fitted with sliphooks and turnbuckles, which shall be accessible... least 391/2 inches above the cargo. (f) Steering arrangements. (1) Steering arrangements shall be...

Avoiding Internal Capsule Stimulation With a New Eight-Channel Steering Deep Brain Stimulation Lead.

Science.gov (United States)

van Dijk, Kees J; Verhagen, Rens; Bour, Lo J; Heida, Ciska; Veltink, Peter H

2017-10-15

Novel deep brain stimulation (DBS) lead designs are currently entering the market, which are hypothesized to provide a way to steer the stimulation field away from neural populations responsible for side effects and towards populations responsible for beneficial effects. The objective of this study is to assess the performances of a new eight channel steering-DBS lead and compare this with a conventional cylindrical contact (CC) lead. The two leads were evaluated in a finite element electric field model combined with multicompartment neuron and axon models, representing the internal capsule (IC) fibers and subthalamic nucleus (STN) cells. We defined the optimal stimulation setting as the configuration that activated the highest percentage of STN cells, without activating any IC fibers. With this criterion, we compared monopolar stimulation using a single contact of the steering-DBS lead and CC lead, on three locations and four orientations of the lead. In addition, we performed a current steering test case by dividing the current over two contacts with the steering-DBS lead in its worst-case orientation. In most cases, the steering-DBS lead is able to stimulate a significantly higher percentage of STN cells compared to the CC lead using single contact stimulation or using a two contact current steering protocol when there is approximately a 1 mm displacement of the CC lead. The results also show that correct placement and orientation of the lead in the target remains an important aspect in achieving the optimal stimulation outcome. Currently, clinical trials are set up in Europe with a similar design as the steering-DBS lead. Our results illustrate the importance of the orientation of the new steering-DBS lead in avoiding side effects induced by stimulation of IC fibers. Therefore, in clinical trials sufficient attention should be paid to implanting the steering DBS-lead in the most effective orientation. © 2017 International Neuromodulation Society.

Green channel cargo inspection system

International Nuclear Information System (INIS)

Shi Yuanping; Yu Jingsheng; Sun Hongqiang; Hao Pu; Cai Wenxia

2011-01-01

A radiation detection device was installed in the lanes of a highway toll station, radioactive rays which was collimated emitted through the measured, and arrived the detector. The average density of the fresh agricultural products belonged to Green channel and other prohibited items vary greatly, the absorption of radiation are different between the Green Channel Cargo and other substances. Prior to the experimental group, different standard samples which represent different models and goods were measured, the different standard samples were stored in a computer database. When the trucks get through the Green Channel, the detector will detect the radiation signal and bring to the computer, the computer will process the measured data, and make a conclusion whether the goods are Green Channel cargo. (authors)

Analysis of containerized cargo in the ship container terminal

Energy Technology Data Exchange (ETDEWEB)

Obhodas, Jasmina, E-mail: jobhodas@irb.h [Ruder Boskovic Institute, Bijenicka c. 54, 10000 Zagreb (Croatia); Sudac, Davorin [Ruder Boskovic Institute, Bijenicka c. 54, 10000 Zagreb (Croatia); Valkovic, Vladivoj [A.C.T.d.o.o., Prilesje 4, 10000 Zagreb (Croatia); Baricevic, Martina [Ruder Boskovic Institute, Bijenicka c. 54, 10000 Zagreb (Croatia); Franulovic, Andrej [Croatian Custom, Alexandera von Humboldta 4, 10000 Zagreb (Croatia); Perot, Bertrand; Carasco, Cedric; Alain, Mariani; Anne-Cecile, Raoux; El Kanawati, Wassila [CEA, DEN, Cadarache, Nuclear Measurement Laboratory, F-13108 Saint-Paul-lez-Durance (France)

2010-07-21

The container scanning system based on the elemental analysis of suspect cargo by use of fast 14 MeV neutrons with detection of associated alpha particle has been recently developed under the EU-FP6 'EURITRACK' project. The system is currently set-up in port of Rijeka, Croatia, to be tested and upgraded under the EU-JLS ERITR-C project. Here we present results of analysis performed on 152 screened containers selected by the Croatian Custom and detail descriptive statistical analysis of their shipping manifests. Statistics such as distribution functions of container types, type of packages, type of cargos and average cargo densities were obtained in order to establish the properties of standard cargo traffic. The analyses by using multivariate statistical approach that included principal component analysis (PCA) and between group analysis (BGA) have shown that cargo matrices can be classified as metallic (Fe, Al, Cu, Zn, Ni...), ceramic-glass (Si, O) and organic (C, O, N). In this paper the organic matrices have been analysed in more detail since they are identified as most difficult because of their potential interference with main elements contained in threat materials such as explosives or drugs. Density as a discriminating factor has been included for better recognition of different types of goods.

Genetic algorithm–based varying parameter linear quadratic regulator control for four-wheel independent steering vehicle

Directory of Open Access Journals (Sweden)

Linlin Gao

2015-11-01

Full Text Available From the perspective of vehicle dynamics, the four-wheel independent steering vehicle dynamics stability control method is studied, and a four-wheel independent steering varying parameter linear quadratic regulator control system is proposed with the help of expert control method. In the article, a four-wheel independent steering linear quadratic regulator controller for model following purpose is designed first. Then, by analyzing the four-wheel independent steering vehicle dynamic characteristics and the influence of linear quadratic regulator control parameters on control performance, a linear quadratic regulator control parameter adjustment strategy based on vehicle steering state is proposed to achieve the adaptive adjustment of linear quadratic regulator control parameters. In addition, to further improve the control performance, the proposed varying parameter linear quadratic regulator control system is optimized by genetic algorithm. Finally, simulation studies have been conducted by applying the proposed control system to the 8-degree-of-freedom four-wheel independent steering vehicle dynamics model. The simulation results indicate that the proposed control system has better performance and robustness and can effectively improve the stability and steering safety of the four-wheel independent steering vehicle.

46 CFR 153.1000 - Special operating requirements for cargoes reactive with water.

Science.gov (United States)

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Special operating requirements for cargoes reactive with water. 153.1000 Section 153.1000 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED... MATERIALS Operations Special Cargo Procedures § 153.1000 Special operating requirements for cargoes reactive...

Detection of fissionable materials in cargoes using monochromatic photon radiography

Science.gov (United States)

Danagoulian, Areg; Lanza, Richard; O'Day, Buckley; LNSP Team

2015-04-01

The detection of Special Nuclear Materials (e.g. Pu and U) and nuclear devices in the commercial cargo traffic is one of the challenges posed by the threat of nuclear terrorism. Radiography and active interrogation of heavily loaded cargoes require ~ 1 - 10MeV photons for penetration. In a proof-of-concept system under development at MIT, the interrogating monochromatic photon beam is produced via a 11B(d , nγ) 12C reaction. To achieve this, a boron target is used along with the 3 MeV d+ RFQ accelerator at MIT-Bates. The reactions results in the emission of very narrow 4.4 MeV and 15.1 MeV gammas lines. The photons, after traversing the cargo, are detected by an array of NaI(Tl) detectors. A spectral analysis of the transmitted gammas allows to independently determine the areal density and the atomic number (Z) of the cargo. The proposed approach could revolutionize cargo inspection, which, in its current fielded form has to rely on simple but high dose bremsstrahlung sources. Use of monochromatic sources would significantly reduce the necessary dose and allow for better determination of the cargo's atomic number. The general methodology will be described and the preliminary results from the proof-of-concept system will be presented and discussed. Supported by NSF/DNDO Collaborative Research ARI-LA Award ECCS-1348328.

Effects of slow-release urea on ruminal digesta characteristics and growth performance in beef steers

DEFF Research Database (Denmark)

Taylor-Edwards, C C; Hibbard, G; Kitts, S E

2009-01-01

Two experiments were conducted to evaluate the effects of slow urea (SRU) versus feed-grade urea on ruminal metabolite characteristics in steers and DMI, gain, and G:F in growing beef steers.......Two experiments were conducted to evaluate the effects of slow urea (SRU) versus feed-grade urea on ruminal metabolite characteristics in steers and DMI, gain, and G:F in growing beef steers....

A novel mouse model carrying a human cytoplasmic dynein mutation shows motor behavior deficits consistent with Charcot-Marie-Tooth type 2O disease.

Science.gov (United States)

Sabblah, Thywill T; Nandini, Swaran; Ledray, Aaron P; Pasos, Julio; Calderon, Jami L Conley; Love, Rachal; King, Linda E; King, Stephen J

2018-01-29

Charcot-Marie-Tooth disease (CMT) is a peripheral neuromuscular disorder in which axonal degeneration causes progressive loss of motor and sensory nerve function. The loss of motor nerve function leads to distal muscle weakness and atrophy, resulting in gait problems and difficulties with walking, running, and balance. A mutation in the cytoplasmic dynein heavy chain (DHC) gene was discovered to cause an autosomal dominant form of the disease designated Charcot-Marie-Tooth type 2 O disease (CMT2O) in 2011. The mutation is a single amino acid change of histidine into arginine at amino acid 306 (H306R) in DHC. In order to understand the onset and progression of CMT2, we generated a knock-in mouse carrying the corresponding CMT2O mutation (H304R/+). We examined H304R/+ mouse cohorts in a 12-month longitudinal study of grip strength, tail suspension, and rotarod assays. H304R/+ mice displayed distal muscle weakness and loss of motor coordination phenotypes consistent with those of individuals with CMT2. Analysis of the gastrocnemius of H304R/+ male mice showed prominent defects in neuromuscular junction (NMJ) morphology including reduced size, branching, and complexity. Based on these results, the H304R/+ mouse will be an important model for uncovering functions of dynein in complex organisms, especially related to CMT onset and progression.

Auditory orientation in crickets: Pattern recognition controls reactive steering

Science.gov (United States)

Poulet, James F. A.; Hedwig, Berthold

2005-10-01

Many groups of insects are specialists in exploiting sensory cues to locate food resources or conspecifics. To achieve orientation, bees and ants analyze the polarization pattern of the sky, male moths orient along the females' odor plume, and cicadas, grasshoppers, and crickets use acoustic signals to locate singing conspecifics. In comparison with olfactory and visual orientation, where learning is involved, auditory processing underlying orientation in insects appears to be more hardwired and genetically determined. In each of these examples, however, orientation requires a recognition process identifying the crucial sensory pattern to interact with a localization process directing the animal's locomotor activity. Here, we characterize this interaction. Using a sensitive trackball system, we show that, during cricket auditory behavior, the recognition process that is tuned toward the species-specific song pattern controls the amplitude of auditory evoked steering responses. Females perform small reactive steering movements toward any sound patterns. Hearing the male's calling song increases the gain of auditory steering within 2-5 s, and the animals even steer toward nonattractive sound patterns inserted into the speciesspecific pattern. This gain control mechanism in the auditory-to-motor pathway allows crickets to pursue species-specific sound patterns temporarily corrupted by environmental factors and may reflect the organization of recognition and localization networks in insects. localization | phonotaxis

46 CFR 185.320 - Steering gear, controls, and communication system tests.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Steering gear, controls, and communication system tests. 185.320 Section 185.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) OPERATIONS Miscellaneous Operating Requirements § 185.320 Steering...

49 CFR 1546.205 - Acceptance and screening of cargo.

Science.gov (United States)

2010-10-01

... 49 Transportation 9 2010-10-01 2010-10-01 false Acceptance and screening of cargo. 1546.205... SECURITY Operations § 1546.205 Acceptance and screening of cargo. (a) Preventing or deterring the carriage... aircraft. (2) Prevents access by unauthorized persons other than an authorized foreign air carrier employee...

Development of sup 6 sup 0 Co cargo train inspection system

CERN Document Server

Wu Zhi Fang

2002-01-01

The author introduces the research and development of sup 6 sup 0 Co cargo train inspection system. With the use of radiography principle, every car image is acquired when the cargo train runs through the inspection channel. It is evaluated whether the cargo in car matches the corresponding customs declaration information with digital image processing techniques. The system has been installed in railway port at Manzhouli Customs

Chromium supplementation enhances the acute phase response of steers to a lipopolysaccharide (LPS) challenge

Science.gov (United States)

The study examined the effect of chromium supplementation on the response of steers to an LPS challenge. Twenty crossbred steers (235±4 kg BW) received 0 ppb (Control; C) or 200 ppb chromium propionate (CHR) for 55 days. Steers were fitted with jugular catheters and rectal temperature (RT) recording...

Throughput-Based Traffic Steering in LTE-Advanced HetNet Deployments

DEFF Research Database (Denmark)

Gimenez, Lucas Chavarria; Kovacs, Istvan Z.; Wigard, Jeroen

2015-01-01

The objective of this paper is to propose traffic steering solutions that aim at optimizing the end-user throughput. Two different implementations of an active mode throughput-based traffic steering algorithm for Heterogeneous Networks (HetNet) are introduced. One that always forces handover of t...... throughput is generally higher, reaching values of 36% and 18% for the medium- and high-load conditions....

On Using Current Steering Logic in Mixed Analogue-digital Circuits

DEFF Research Database (Denmark)

Lehmann, Torsten

1998-01-01

The authors investigate power supply noise in mixed analogue-digital circuits, arising from communication between the analogue and digital parts of the circuit. Current steering techniques and proper buffering are used to show which noise currents can be reduced and which cannot. In addition......, a high-swing current steering buffer for driving analogue switches or external digital signals is proposed....

Conditional steering under the von Neumann scenario

Science.gov (United States)

Mukherjee, Kaushiki; Paul, Biswajit; Karmakar, Sumana; Sarkar, Debasis; Mukherjee, Amit; Bhattacharya, Some Sankar; Roy, Arup

2017-08-01

In Phys. Lett. A 166, 293 (1992), 10.1016/0375-9601(92)90711-T, Popescu and Rohrlich characterized nonlocality of pure n -partite entangled systems by studying bipartite violation of local realism when n -2 number of parties perform projective measurements on their particles. A pertinent question in this scenario is whether similar characterization is possible for n -partite mixed entangled states also. In the present work we have followed an analogous approach so as to explore whether given a tripartite mixed entangled state the conditional bipartite states obtained by performing projective measurement on the third party demonstrate a weaker form of nonlocality, quantum steering. We also compare this phenomenon of conditional steering with existing notions of tripartite correlations.

Regulation of microtubule-based transport by MAP4

Science.gov (United States)

Semenova, Irina; Ikeda, Kazuho; Resaul, Karim; Kraikivski, Pavel; Aguiar, Mike; Gygi, Steven; Zaliapin, Ilya; Cowan, Ann; Rodionov, Vladimir

2014-01-01

Microtubule (MT)-based transport of organelles driven by the opposing MT motors kinesins and dynein is tightly regulated in cells, but the underlying molecular mechanisms remain largely unknown. Here we tested the regulation of MT transport by the ubiquitous protein MAP4 using Xenopus melanophores as an experimental system. In these cells, pigment granules (melanosomes) move along MTs to the cell center (aggregation) or to the periphery (dispersion) by means of cytoplasmic dynein and kinesin-2, respectively. We found that aggregation signals induced phosphorylation of threonine residues in the MT-binding domain of the Xenopus MAP4 (XMAP4), thus decreasing binding of this protein to MTs. Overexpression of XMAP4 inhibited pigment aggregation by shortening dynein-dependent MT runs of melanosomes, whereas removal of XMAP4 from MTs reduced the length of kinesin-2–dependent runs and suppressed pigment dispersion. We hypothesize that binding of XMAP4 to MTs negatively regulates dynein-dependent movement of melanosomes and positively regulates kinesin-2–based movement. Phosphorylation during pigment aggregation reduces binding of XMAP4 to MTs, thus increasing dynein-dependent and decreasing kinesin-2–dependent motility of melanosomes, which stimulates their accumulation in the cell center, whereas dephosphorylation of XMAP4 during dispersion has an opposite effect. PMID:25143402

46 CFR 122.320 - Steering gear, controls, and communication system tests.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Steering gear, controls, and communication system tests... communication system tests. The master of a vessel shall have examined and tested the steering gear, signaling whistle, propulsion controls, and communication systems of the vessel prior to getting underway for a...

Steering disturbance rejection using a physics-based neuromusculoskeletal driver model

Science.gov (United States)

Mehrabi, Naser; Sharif Razavian, Reza; McPhee, John

2015-10-01

The aim of this work is to develop a comprehensive yet practical driver model to be used in studying driver-vehicle interactions. Drivers interact with their vehicle and the road through the steering wheel. This interaction forms a closed-loop coupled human-machine system, which influences the driver's steering feel and control performance. A hierarchical approach is proposed here to capture the complexity of the driver's neuromuscular dynamics and the central nervous system in the coordination of the driver's upper extremity activities, especially in the presence of external disturbance. The proposed motor control framework has three layers: the first (or the path planning) plans a desired vehicle trajectory and the required steering angles to perform the desired trajectory; the second (or the musculoskeletal controller) actuates the musculoskeletal arm to rotate the steering wheel accordingly; and the final layer ensures the precision control and disturbance rejection of the motor control units. The physics-based driver model presented here can also provide insights into vehicle control in relaxed and tensed driving conditions, which are simulated by adjusting the driver model parameters such as cognition delay and muscle co-contraction dynamics.

Effects of clay on fat necrosis and carcass characteristics in Japanese Black steers.

Science.gov (United States)

Oka, Akio; Iwamoto, Eiji; Tatsuda, Ken

2015-10-01

Twenty 10-month-old Japanese Black steers were used to evaluate the effects of clay on fat necrosis and carcass characteristics. Ten steers (Clay group) were fed the clay (50 g/day) during 10-30 months of age. The other 10 steers (Control group) were not fed it. There was no significant difference in body weight or average daily gain between the two groups (P > 0.05). The occurrence of fat necrotic mass in the Clay group (30%) was lower (P Clay group was smaller (P clay prevented the occurrence of fat necrosis and did not affect the carcass characteristics in Japanese Black steers. © 2015 Japanese Society of Animal Science.

Slow-release urea in supplement fed to beef steers

Directory of Open Access Journals (Sweden)

Ana Paula Gonçalves

2015-02-01

Full Text Available Replacing regular urea (RU by slow-release urea (SRU at two levels of non-protein nitrogen (NPN in concentrate, offered with low-quality roughage, was evaluated in beef steers on dry matter intake (DMI, ruminal fermentation parameters, plasma urea nitrogen (PUN, total tract apparent digestibility of diets and in situ degradability of nitrogen sources. Eight ruminally cannulated steers were allocated into two 4x4 Latin squares, totalizing four treatments: 40 NPN/0 SRU: 40% of concentrate crude protein (CP as NPN, resulting from 0% of SRU and 100% of RU; 40 NPN/50 SRU: 40% of concentrate CP as NPN, resulting from 50% of SRU and 50% of RU; 40 NPN/100 SRU: 40% of concentrate CP as NPN, resulting from 100% of SRU and 0% of RU; 80 NPN/100 SRU: 80% of concentrate CP as NPN, resulting from 100% of SRU and 0% of RU. Results showed that partial substitution of regular urea by slow-release urea did not alter dry matter intake, pattern of ruminal fermentation or plasma urea nitrogen concentrations and increased the total tract apparent digestibility of crude protein in steers diets. The increase in non-protein nitrogen content in crude protein of the concentrate could compromise feed intake and the efficiency of nutrient utilization in the steers fed complete diets based on low quality forage.

Static load simulation of steering knuckle for a formula student race car

Science.gov (United States)

Saputro, Bagus Aulia; Ubaidillah, Triono, Dicky Agus; Pratama, Dzaky Roja; Cahyono, Sukmaji Indro; Imaduddin, Fitrian

2018-02-01

This research aims to determine the stress distribution which occurs on the steering knuckle and to define its safety factor number. Steering knuckle is the most critical part of a car's steering system. Steering knuckle supports the tie rod, brake caliper, and the wheels to provide stability. Steering knuckle withstands the load which given on the front wheels and functions as the wheel's axis. Balljoint and king support the rotation of the suspension arm. When the car is in idle position, knuckle hold the weight of the car, it gets braking force when it's braking and cornering. Knuckle is designed to have the strength that could withstand load and to have a good safety factor value. Knuckle is designed using Fusion software then simulated using Fusion simulation software with a static load, moment braking force, and cornering force as the loads in this simulation. The simulation works in ideal condition. The result of this simulation is satisfying. This simulation produces a maximum displacement of 0.01281mm, the maximum shear stress is 3.707 MPa on the stub hole, and the safety factor is 5.24. The material used for this product is mild steel AISI 1018.

Non-Mechanical Beam Steering in Free-Space Optical Communication Transceivers

Science.gov (United States)

Shortt, Kevin

Free-space optical communications systems are a rapidly growing field as they carry many of the advantages of traditional fibre-based communications systems without the added investment of installing complex infrastructure. Moreover, these systems are finding key niches in mobile platforms in order to take advantage of the increased bandwidth over traditional RF systems. Of course, the inevitable problem of tracking arises when dealing with mobile stations. To compound the problem in the case of communications to low Earth or geosynchronous orbits, FSOC systems typically operate with tightly confined beams over great distances often requiring pointing accuracies on the order of micro-radians or smaller. Mechanisms such as gimbal mounts and fine-steering mirrors are the usual candidates for platform stabilization, however, these clearly have substantial power requirements and inflate the mass of the system. Spatial light modulators (also known as optical phased arrays), on the other hand, offer a suitable alternative for beam-pointing stabilization. Some of the advantages of spatial light modulators over fine-steering mirrors include programmable multiple simultaneous beams, dynamic focus/defocus and moderate to excellent optical power handling capability. This thesis serves as an investigation into the implementation of spatial light modulators as a replacement for traditional fine-steering mirrors in the fine-pointing subsystem. In particular, pointing accuracy and scanning ability will be highlighted as performance metrics in the context of a variety of communication scenarios. Keywords: Free-space optical communications, beam steering, fine-steering mirror, spatial light modulator, optical phased array.

Beam steered millimeter-wave fiber-wireless system for 5G indoor coverage

NARCIS (Netherlands)

Cao, Z.; Wang, Q.; Tessema, N.M.; Leijtens, X.J.M.; Soares, F.M.; Koonen, A.M.J.

2016-01-01

A 38GHz beam steered fiber-wireless system, enabled by a novel integrated optical tunable delay line, is demonstrated for 5G indoor coverage. The beam steering gains 14dBm spatial power focusing and 6 times EVM improvement.

Development of a Computational Steering Framework for High Performance Computing Environments on Blue Gene/P Systems

KAUST Repository

Danani, Bob K.

2012-07-01

Computational steering has revolutionized the traditional workflow in high performance computing (HPC) applications. The standard workflow that consists of preparation of an application’s input, running of a simulation, and visualization of simulation results in a post-processing step is now transformed into a real-time interactive workflow that significantly reduces development and testing time. Computational steering provides the capability to direct or re-direct the progress of a simulation application at run-time. It allows modification of application-defined control parameters at run-time using various user-steering applications. In this project, we propose a computational steering framework for HPC environments that provides an innovative solution and easy-to-use platform, which allows users to connect and interact with running application(s) in real-time. This framework uses RealityGrid as the underlying steering library and adds several enhancements to the library to enable steering support for Blue Gene systems. Included in the scope of this project is the development of a scalable and efficient steering relay server that supports many-to-many connectivity between multiple steered applications and multiple steering clients. Steered applications can range from intermediate simulation and physical modeling applications to complex computational fluid dynamics (CFD) applications or advanced visualization applications. The Blue Gene supercomputer presents special challenges for remote access because the compute nodes reside on private networks. This thesis presents an implemented solution and demonstrates it on representative applications. Thorough implementation details and application enablement steps are also presented in this thesis to encourage direct usage of this framework.

77 FR 36008 - Agency Information Collection Activities; Proposed Collection: Cargo Theft Incident Report...

Science.gov (United States)

2012-06-15

... Collection Activities; Proposed Collection: Cargo Theft Incident Report, Revision of a Currently Approved... collection: Revision of a currently approved collection. (2) The title of the form/collection: Cargo Theft... enforcement agencies. Brief Abstract: This collection is needed to collect information on cargo theft...

Optimization of steering elements in the RIA driver linac

International Nuclear Information System (INIS)

Lessner, E. S.; Aseev, V. S.; Ostroumov, P. N.; Physics

2005-01-01

The driver linac of the projected RIA facility is a versatile accelerator, a 1.4-GV, CW superconducting (SC) linac designed to simultaneously accelerate several heavy-ion charge states, providing beams from proton to uranium at 400 MeV/u at power levels at a minimum of 100 kW and up to 400 kW for most beams. Acceleration of multiple-charge-state uranium beams places stringent requirements on the linac design. A steering algorithm was derived that fulfilled the driver's real estate requirements, such as placement of steering dipole coils on SC solenoids and of beam position monitors outside cryostats, and beam-dynamics requirements, such as coupling effects induced by the focusing solenoids. The algorithm has been fully integrated into the tracking code TRACK and it is used to study and optimize the number and position of steering elements that minimize the multiple-beam centroid oscillations and preserve the beam emittance under misalignments of accelerating and transverse focusing elements in the driver linac

Fermilab Steering Group Report

Energy Technology Data Exchange (ETDEWEB)

Beier, Eugene; /Pennsylvania U.; Butler, Joel; /Fermilab; Dawson, Sally; /Brookhaven; Edwards, Helen; /Fermilab; Himel, Thomas; /SLAC; Holmes, Stephen; /Fermilab; Kim, Young-Kee; /Fermilab /Chicago U.; Lankford, Andrew; /UC, Irvine; McGinnis, David; /Fermilab; Nagaitsev, Sergei; /Fermilab; Raubenheimer, Tor; /SLAC /Fermilab

2007-01-01

The Fermilab Steering Group has developed a plan to keep U.S. accelerator-based particle physics on the pathway to discovery, both at the Terascale with the LHC and the ILC and in the domain of neutrinos and precision physics with a high-intensity accelerator. The plan puts discovering Terascale physics with the LHC and the ILC as Fermilab's highest priority. While supporting ILC development, the plan creates opportunities for exciting science at the intensity frontier. If the ILC remains near the Global Design Effort's technically driven timeline, Fermilab would continue neutrino science with the NOVA experiment, using the NuMI (Neutrinos at the Main Injector) proton plan, scheduled to begin operating in 2011. If ILC construction must wait somewhat longer, Fermilab's plan proposes SNuMI, an upgrade of NuMI to create a more powerful neutrino beam. If the ILC start is postponed significantly, a central feature of the proposed Fermilab plan calls for building an intense proton facility, Project X, consisting of a linear accelerator with the currently planned characteristics of the ILC combined with Fermilab's existing Recycler Ring and the Main Injector accelerator. The major component of Project X is the linac. Cryomodules, radio-frequency distribution, cryogenics and instrumentation for the linac are the same as or similar to those used in the ILC at a scale of about one percent of a full ILC linac. Project X's intense proton beams would open a path to discovery in neutrino science and in precision physics with charged leptons and quarks. World-leading experiments would allow physicists to address key questions of the Quantum Universe: How did the universe come to be? Are there undiscovered principles of nature: new symmetries, new physical laws? Do all the particles and forces become one? What happened to the antimatter? Building Project X's ILC-like linac would offer substantial support for ILC development by accelerating the

Steering, Entanglement, Nonlocality, and the EPR Paradox

Science.gov (United States)

Wiseman, Howard; Jones, Steve; Andrew, Doherty

2007-06-01

The concept of steering was introduced by Schroedinger in 1935 as a generalization of the EPR paradox for arbitrary pure bipartite entangled states and arbitrary measurements by one party. Until now, it has never been rigorously defined, so it has not been known (for example) what mixed states are steerable (that is, can be used to exhibit steering). We provide an operational definition, from which we prove (by considering Werner states and Isotropic states) that steerable states are a strict subset of the entangled states, and a strict superset of the states that can exhibit Bell-nonlocality. For arbitrary bipartite Gaussian states we derive a linear matrix inequality that decides the question of steerability via Gaussian measurements, and we relate this to the original EPR paradox.

SPS Beam Steering for LHC Extraction

Energy Technology Data Exchange (ETDEWEB)

Gianfelice-Wendt, Eliana [Fermilab; Bartosik, Hannes [CERN; Cornelis, Karel [CERN; Norderhaug Drøsdal, Lene [CERN; Goddard, Brennan [CERN; Kain, Verena [CERN; Meddahi, Malika [CERN; Papaphilippou, Yannis [CERN; Wenninger, Jorg [CERN

2014-07-01

The CERN Super Proton Synchrotron accelerates beams for the Large Hadron Collider to 450 GeV. In addition it produces beams for fixed target facilities which adds complexity to the SPS operation. During the run 2012-2013 drifts of the extracted beam trajectories have been observed and lengthy optimizations in the transfer lines were performed to reduce particle losses in the LHC. The observed trajectory drifts are consistent with the measured SPS orbit drifts at extraction. While extensive studies are going on to understand, and possibly suppress, the source of such SPS orbit drifts the feasibility of an automatic beam steering towards a “golden” orbit at the extraction septa, by means of the interlocked correctors, is also being investigated. The challenges and constraints related to the implementation of such a correction in the SPS are described. Simulation results are presented and a possible operational steering strategy is proposed.

Pointing Device Performance in Steering Tasks.

Science.gov (United States)

Senanayake, Ransalu; Goonetilleke, Ravindra S

2016-06-01

Use of touch-screen-based interactions is growing rapidly. Hence, knowing the maneuvering efficacy of touch screens relative to other pointing devices is of great importance in the context of graphical user interfaces. Movement time, accuracy, and user preferences of four pointing device settings were evaluated on a computer with 14 participants aged 20.1 ± 3.13 years. It was found that, depending on the difficulty of the task, the optimal settings differ for ballistic and visual control tasks. With a touch screen, resting the arm increased movement time for steering tasks. When both performance and comfort are considered, whether to use a mouse or a touch screen for person-computer interaction depends on the steering difficulty. Hence, a input device should be chosen based on the application, and should be optimized to match the graphical user interface. © The Author(s) 2016.

Calculation of the beam injector steering system using Helmholtz coils

International Nuclear Information System (INIS)

Passaro, A.; Sircilli Neto, F.; Migliano, A.C.C.

1991-03-01

In this work, a preliminary evaluation of the beam injector steering system of the IEAv electron linac is presented. From the existing injector configuration and with the assumptions of monoenergetic beam (100 keV) and uniform magnetic field, two pairs of Helmholtz coils were calculated for the steering system. Excitations of 105 A.turn and 37 A.turn were determined for the first and second coils, respectively. (author)

A Vehicle Haptic Steering by Wire System Based on High Gain GPI Observers

Directory of Open Access Journals (Sweden)

A. Rodriguez-Angeles

2014-01-01

Full Text Available A vehicle steering by wire (SBW haptic system based on high gain generalized proportional integral (GPI observers is introduced. The observers are considered for the estimation of dynamic perturbations that are present at the tire and steering wheel. To ensure efficient tracking between the commanded steering wheel angle and the tire orientation angle, the estimated perturbations are on line canceled. As to provide a haptic interface with the driver, the estimated dynamic effects at the steering rack are fed back to the steering wheel, yielding a master-slave haptic system with bilateral communication. For implementation purposes few sensors and minimum knowledge of the dynamic model are required, which is a major advantage compared to other approaches. Only position tracking errors are fed back, while all other signals are estimated by the high gain GPI observers. The scheme is robust to uncertainty on the input gain and cancels dynamic perturbation effects such as friction and aligning forces on the tire. Experimental results are presented on a prototype platform.

Arbitrary vehicle steering characteristics with changing ratio rack and pinion transmission

Directory of Open Access Journals (Sweden)

András G Bendefy

2015-12-01

Full Text Available In order to achieve arbitrary steering characteristics at vehicles, a steering mechanism was developed, in which changing ratio rack and pinion connections have been applied. In contrary to a regular steering mechanism where only a single rack is used, two racks were applied in order to make arbitrary characteristics possible. The turning wheel’s required motion functions had to be defined first, thereafter could we determine the changing ratio rack and pinion geometry which produces this motion. First, a simplified two-dimensional mockup was created in order to study the difficulties and possibilities of a real construction. Later, a fully functional assembly was designed and manufactured to make further experiments possible.

NanoShuttles: Harnessing Motor Proteins to Transport Cargo in Synthetic Environments

Science.gov (United States)

Vogel, V.; Hess, H.

Motors have become a crucial commodity in our daily lives, from transportation to driving conveyor belts that enable the sequential assembly of cars and other industrial machines. For the sequential assembly of building blocks at the nanoscale that would not assemble spontaneously into larger functional systems, however, active transport systems are not yet available. In contrast, cells have evolved sophisticated molecular machinery that drives movement and active transport. Driven by the conversion of chemical into mechanical energy, namely through hydrolysis of the biological fuel ATP, molecular motors enable cells to operate far away from equilibrium by transporting organelles and molecules to designated locations within the cell, often against concentration gradients. Inspired by the biological concept of active transport, major efforts are underway to learn how to build nanoscale transport systems that are driven by molecular motors. Emerging engineering principles are discussed of how to build tracks and junctions to guide such nanoshuttles, how to load them with cargo and control their speed, how to use active transport to assemble mesoscopic structures that would otherwise not assemble spontaneously and what polymeric materials to choose to integrate motors into MEMS and other biohybrid devices. Finally, two applications that exploit the physical properties of microtubules are discussed, surface imaging by a swarm of microtubules and a self-assembled picoNewton force meter to probe receptor-ligand interactions.

Reducing Aerodynamic Drag on Empty Open Cargo Vehicles

Science.gov (United States)

Ross, James C.; Storms, Bruce L.; Dzoan, Dan

2009-01-01

Some simple structural modifications have been demonstrated to be effective in reducing aerodynamic drag on vehicles that have empty open cargo bays. The basic idea is to break up the airflow in a large open cargo bay by inserting panels to divide the bay into a series of smaller bays. In the case of a coal car, this involves inserting a small number (typically between two and four) of vertical full-depth or partial-depth panels.

A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells

Directory of Open Access Journals (Sweden)

Amber L. Jolly

2016-01-01

Full Text Available Long-distance intracellular transport of organelles, mRNA, and proteins (“cargo” occurs along the microtubule cytoskeleton by the action of kinesin and dynein motor proteins, but the vast network of factors involved in regulating intracellular cargo transport are still unknown. We capitalize on the Drosophila melanogaster S2 model cell system to monitor lysosome transport along microtubule bundles, which require enzymatically active kinesin-1 motor protein for their formation. We use an automated tracking program and a naive Bayesian classifier for the multivariate motility data to analyze 15,683 gene phenotypes and find 98 proteins involved in regulating lysosome motility along microtubules and 48 involved in the formation of microtubule filled processes in S2 cells. We identify innate immunity genes, ion channels, and signaling proteins having a role in lysosome motility regulation and find an unexpected relationship between the dynein motor, Rab7a, and lysosome motility regulation.

Versatile Loading of Diverse Cargo into Functional Polymer Capsules.

Science.gov (United States)

Richardson, Joseph J; Maina, James W; Ejima, Hirotaka; Hu, Ming; Guo, Junling; Choy, Mei Y; Gunawan, Sylvia T; Lybaert, Lien; Hagemeyer, Christoph E; De Geest, Bruno G; Caruso, Frank

2015-02-01

Polymer microcapsules are of particular interest for applications including self-healing coatings, catalysis, bioreactions, sensing, and drug delivery. The primary way that polymer capsules can exhibit functionality relevant to these diverse fields is through the incorporation of functional cargo in the capsule cavity or wall. Diverse functional and therapeutic cargo can be loaded into polymer capsules with ease using polymer-stabilized calcium carbonate (CaCO 3 ) particles. A variety of examples are demonstrated, including 15 types of cargo, yielding a toolbox with effectively 500+ variations. This process uses no harsh reagents and can take less than 30 min to prepare, load, coat, and form the hollow capsules. For these reasons, it is expected that the technique will play a crucial role across scientific studies in numerous fields.

Geometrodynamic steering principle reveals the determiners of inertia

International Nuclear Information System (INIS)

Wheeler, J.A.

1988-01-01

What shall the authors need to grasp the essence of quantum gravity? One requirement, at least, is essential: to understand the steering principle of classical geometrodynamics. The authors outline here the physical content of that steering principle - heat of the so-called initial value problem - in its J.W. York, Jr. formulation. The central idea epitomizes itself in a single simple sentence: Mass-energy there determines inertia here. They spell out this steering principle both in its precise form and in its poor man's version. At both levels of analysis considerations of physics and mathematics alike require that the effective mass-energy of gravity waves must make itself felt on the spacetime geometry - and therefore on the gyro-defined local inertial frame of reference - on the same level as matter itself. Additional to the (mass)/(distance) Newtonian potential so familiar as measure of the effect of a nearby mass on the local frame is the Thirring and Lense gravitomagnetic potential, proportional to (angular momentum) x (distance vector)/(distance). The recent proposal of Ciufolini for a dual laser-ranged LAGEOS satellite to detect the thus-predicted gravitomagnetism of the earth is briefly described

Asphyxiation death caused by oxygen-depleting cargo on a ship.

Science.gov (United States)

Sundal, Marjana Kjetland; Lilleng, Peer Kaare; Barane, Hans; Morild, Inge; Vevelstad, Merete

2017-10-01

The extreme danger associated with entering enclosed spaces loaded with oxygen-depleting organic cargo in ships and tanks is obviously underestimated, both among crew and management. We present a case report to highlight this occupational hazard and to increase the knowledge about the imperative precautions, in order to prevent future accidents. An experienced customs officer was found lifeless at the bottom of the unattended cargo hold on a ship loaded with woodchips. The oxygen content in the cargo atmosphere was below 2%, which is incompatible with life. Forensic autopsy revealed injuries related to the fall, and there were no positive toxicological findings in blood, lung or urine. Management and workers must be taught about the extreme rapidity of developing unconsciousness and asphyxiant death when entering enclosed spaces loaded with oxygen-depleting cargo. Even a single inhalation can result in unconsciousness and death. Dozens of annual deaths and severe injuries can easily be prevented if simple precautions are followed. Copyright © 2017 Elsevier B.V. All rights reserved.

Modern windships. Phase 1

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-11-01

Knud E. Hansen A/S (KEH) has, partly funded by the Energy Research Programme, (EFP-95) investigated in the possibilities of using windships for transportation of cargo on long routes, i.e. across the Atlantic or Pacific Ocean. The task was to peruse experiences from projects made during the past 30 years and then, based on new materials and design principle to make proposals to modern wind driven ships to transportation of cargo - especially bulk carriers. KEH has thus prepared a suggestion for a 50,000 DWT wind driven bulk carrier on these terms. The bulk carrier in question is a modern rig inspired by the classical lugger and junk sail with a total sail area of abt. 10,000 m{sup 2}. The hull of the ship has been developed in order to limit wave resistance and drifting. Project Windship has, in contrast to earlier tests and projects, designed a bulk carrier based on a complete evaluation of ecology, safety, economy and reasonable transportation speed. The research project shows that international sea transportation, with wind as the primary source of energy, does not seem to run up against any obstacles as regards safety. The economical analyses show that windship transportation, with today`s oil prices, will be about 10% higher compared to the diesel driven transportation. In the light of the positive results of the research project the steering committee recommends that phase II of project `Modern Windships` is carried out. (EG)

Modern windships. Phase 1

International Nuclear Information System (INIS)

1996-11-01

Knud E. Hansen A/S (KEH) has, partly funded by the Energy Research Programme, (EFP-95) investigated in the possibilities of using windships for transportation of cargo on long routes, i.e. across the Atlantic or Pacific Ocean. The task was to peruse experiences from projects made during the past 30 years and then, based on new materials and design principle to make proposals to modern wind driven ships to transportation of cargo - especially bulk carriers. KEH has thus prepared a suggestion for a 50,000 DWT wind driven bulk carrier on these terms. The bulk carrier in question is a modern rig inspired by the classical lugger and junk sail with a total sail area of abt. 10,000 m 2 . The hull of the ship has been developed in order to limit wave resistance and drifting. Project Windship has, in contrast to earlier tests and projects, designed a bulk carrier based on a complete evaluation of ecology, safety, economy and reasonable transportation speed. The research project shows that international sea transportation, with wind as the primary source of energy, does not seem to run up against any obstacles as regards safety. The economical analyses show that windship transportation, with today's oil prices, will be about 10% higher compared to the diesel driven transportation. In the light of the positive results of the research project the steering committee recommends that phase II of project 'Modern Windships' is carried out. (EG)

Packaging consideration of two-dimensional polymer-based photonic crystals for laser beam steering

Science.gov (United States)

Dou, Xinyuan; Chen, Xiaonan; Chen, Maggie Yihong; Wang, Alan Xiaolong; Jiang, Wei; Chen, Ray T.

2009-02-01

In this paper, we report the theoretical study of polymer-based photonic crystals for laser beam steering which is based on the superprism effect as well as the experiment fabrication of the two dimensional photonic crystals for the laser beam steering. Superprism effect, the principle for beam steering, was separately studied in details through EFC (Equifrequency Contour) analysis. Polymer based photonic crystals were fabricated through double exposure holographic interference method using SU8-2007. The experiment results were also reported.

A hybrid system for beam steering and wavefront control

Science.gov (United States)

Nikulin, Vladimir V.

2004-06-01

Performance of long-range mobile laser systems operating within Earth's atmosphere is generally limited by several factors. Movement of the communicating platforms, such as aircraft, terrain vehicles, etc., complemented by mechanical vibrations, is the main cause of pointing errors. In addition, atmospheric turbulence causes changes of the refractive index along the propagation path that lead to phase distortions (aberrations), thus creating random redistribution of optical energy in the spatial domain. The combined effect of these factors leads to an increased bit-error probability under adverse operation conditions. While traditional approaches provide separate treatment of these problems, suggesting the development of high-bandwidth beam steering systems to perform tracking and jitter rejection, and wavefront control for the mitigation of atmospheric effects, the two tasks could be integrated. In this paper we present a hybrid laser beam steering/wavefront control system comprising an electrically addressed spatial light modulator (SLM) installed on the Omni-Wrist sensor mount platform. The function of the Omni-Wrist is to provide coarse steering over a wide range of pointing angles, while the purpose of the SLM is twofold: it performs wavefront correction and fine steering. The control law for the Omni-Wrist is synthesized using the decentralized approach that provides independent access to the azimuth and declination channels, while the algorithm for calculating the required phase profile for the SLM is optimization-based. This paper presents the control algorithms, the approach to coordinating the operation of the both systems and the simulation results.

Screening Cargo Containers to Remove a Terrorist Threat

International Nuclear Information System (INIS)

Hazi, A

2005-01-01

Each year some 48 million cargo containers move between the world's ports. More than 6 million of these enter the U.S., but only about 2 percent are opened and inspected when they arrive at U.S. seaports. The West Coast ports of Los Angeles-Long Beach, Oakland, and Seattle alone process 11,000 containers per day, or about 8 containers per minute. Because of this high traffic volume, U.S. seaports are especially vulnerable to a terrorist attack. Illicit radioactive materials could be hidden in any one of the cargo-filled containers that arrive at U.S. ports. Yet, searching every shipment would be bring legitimate commercial activities to a halt. Improving security at U.S. ports is thus one of the nation's most difficult technical and practical challenges because the systems developed for screening cargo must operate in concert with ongoing seaport activities. Working at this intersection of commerce and national security, Lawrence Livermore researchers are applying their expertise in radiation science and detection to develop improved technologies for detecting hidden radioactive materials. One new technology being designed and tested at the Laboratory is a neutron interrogation system for cargo containers. This system will quickly screen incoming shipments to ensure that nuclear materials such as plutonium and highly enriched uranium (HEU) are not smuggled into the U.S

Investigating Block-Copolymer Micelle Dynamics for Tunable Cargo Delivery

Science.gov (United States)

Li, Xiuli; Kidd, Bryce; Cooksey, Tyler; Robertson, Megan; Madsen, Louis

Block-copolymer micelles (BCPMs) can carry molecular cargo in a nanoscopic package that is tunable using polymer structure in combination with cargo properties, as well as with external stimuli such as temperature or pH. For example, BCPMs can be used in targeted anticancer drug delivery due to their biocompatibility, in vivo degradability and prolonged circulation time. We are using NMR spectroscopy and diffusometry as well as SANS to investigate BCPMs. Here we study a diblock poly(ethylene oxide)-b-(caprolactone) (PEO-PCL) that forms spherical micelles at 1% (w/v) in the mixed solvent D2O/THF-d8. We quantify the populations and diffusion coefficients of coexisting micelles and free unimers over a range of temperatures and solvent compositions. We use temperature as a stimulus to enhance unimer exchange and hence trigger cargo release, in some cases at a few degrees above body temperature. We present evidence for dominance of the insertion-expulsion mechanism of unimer exchange in these systems, and we map phase diagrams versus temperature and solvent composition. This study sheds light on how intermolecular interactions fundamentally affect cargo release, unimer exchange, and overall micelle tunability.

46 CFR 91.60-5 - Cargo Ship Safety Construction Certificate.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate. 91.60-5... VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 91.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage...

46 CFR 189.60-10 - Cargo Ship Safety Equipment Certificate.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Cargo Ship Safety Equipment Certificate. 189.60-10... VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 189.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage...

46 CFR 189.60-5 - Cargo Ship Safety Construction Certificate.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate. 189.60-5... VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 189.60-5 Cargo Ship Safety Construction Certificate. (a) All vessels on an international voyage...

Enhancement of the acute phase response to lipopolysaccharide (LPS) challenge in steers supplemented with chromium

Science.gov (United States)

The study examined the effect of chromium supplementation on the response of steers to an LPS challenge. Twenty steers received a premix that added 0 (control) or 0.2 mg/kg of chromium (KemTRACE®brandChromiumProprionate 0.04%, Kemin Industries) to the total diet on a dry matter basis for 55 d. Steer...

Robust H2/H∞ Control for the Electrohydraulic Steering System of a Four-Wheel Vehicle

Directory of Open Access Journals (Sweden)

Min Ye

2014-01-01

Full Text Available To shorten the steer diameter and to improve the maneuverability flexibility of a construction vehicle, four wheels’ steering system is presented. This steering system consists of mechanical-electrical-hydraulic assemblies. Its diagram and principle are depicted in detail. Then the mathematical models are derived step by step, including the whole vehicle model and the hydraulic route model. Considering the nonlinear and time-varying uncertainty of the steering system, robust H2/H∞ controller is put forward to guarantee both the system performance and the robust stability. The H∞ norm of the sensitive function from the parameter perturbation of the hydraulic system to the yaw velocity of the vehicle is taken as the evaluating index of the robustness and the H2 norm of the transfer function from the external disturbance to the steering angle of the wheel as the index of linear quadratic Gaussian. The experimental results showed that the proposed scheme was superior to classical PID controller and can guarantee both the control performance and the robustness of the steering system.

Analysis of Thermal Behavior in a Cargo Hold of LILW Transport Ship

Energy Technology Data Exchange (ETDEWEB)

Lee, Unjang; Kim, Dohyung; Lee, Dongkyu; Choi, Kyusup [Korea Nuclear Engineering and Service Corporation, Seoul (Korea, Republic of)

2007-07-01

With determining Kyongju as a repository site for the low and intermediate-level radioactive waste (LILW), it is time to decide transportation method to bring the waste from NPPs (Nuclear Power Plants) to the site. Now considering transport ship as an alternative, it is important to design cargo compartments in the ship. Especially, it is necessary to ensure thermal criteria in the cargo hold by using natural or forced convection. According to INF Code, there is addressed a technical standard of the cargo that adequate ventilation or refrigeration of enclosed cargo spaces shall be provided so that the average ambient temperature within such spaces does not exceed 55 .deg. C at any time. And many counties which operate LILW transport ships are conformable to the standard, and Ministry of Maritime Affairs and Fisheries of Korea also follows it. In this article analytical study of ventilation system in a cargo hold shows to keep the temperature below 55 .deg. C or not.

CHARACTERISTICS OF THE CARGO INSURANCE CONTRACT IN CASE OF INTERNATIONAL LAND TRANSPORT

Directory of Open Access Journals (Sweden)

Dănilă Ștefan MATEI

2017-05-01

Full Text Available Cargo international transport is an engine for the development of the economic relations between states involving cross-border movement of goods through the crossing of at least one border of a state (international transport or by crossing at least two border crossing points, in which case we are in the presence of an international cargo transit. During the transit the goods transported may be subject to an insurance. The object of the cargo insurance is, thus, represented by the goods, the items expressly listed in the insurance policy, within the territorial limits specified in the insurance policy, both during the transport and during the storage, in the latter case, at the express request of the insured and with the acceptance of the insurer. This paper analyzes the characteristics of the cargo insurance aiming to present the theoretical and practical aspects of interest with regard to the cargo insurance concluded in case of an international land freight transport.

49 CFR 178.348 - Specification DOT 412; cargo tank motor vehicle.

Science.gov (United States)

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 412; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.348 Specification DOT 412; cargo tank motor vehicle. ...

49 CFR 178.347 - Specification DOT 407; cargo tank motor vehicle.

Science.gov (United States)

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 407; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.347 Specification DOT 407; cargo tank motor vehicle. ...

49 CFR 178.346 - Specification DOT 406; cargo tank motor vehicle.

Science.gov (United States)

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Specification DOT 406; cargo tank motor vehicle... SPECIFICATIONS FOR PACKAGINGS Specifications for Containers for Motor Vehicle Transportation § 178.346 Specification DOT 406; cargo tank motor vehicle. ...

Column generation approaches to ship scheduling with flexible cargo sizes

DEFF Research Database (Denmark)

Brønmo, Geir; Nygreen, Bjørn; Lysgaard, Jens

We present a Dantzig-Wolfe procedure for the ship scheduling problem with flexible cargo sizes. This problem is similar to the well-known pickup and delivery problem with time windows, but the cargo sizes are defined by an interval instead of a fixed value. We show that the introduction of flexible...

46 CFR 189.60-15 - Cargo Ship Safety Radio Certificate.

Science.gov (United States)

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Cargo Ship Safety Radio Certificate. 189.60-15 Section... VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 189.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation...

46 CFR 91.60-10 - Cargo Ship Safety Equipment Certificate.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo Ship Safety Equipment Certificate. 91.60-10... VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 91.60-10 Cargo Ship Safety Equipment Certificate. (a) All vessels on an international voyage are...

A matheuristic for the Cargo Mix Problem with Block Stowage

DEFF Research Database (Denmark)

Christensen, Jonas Mark; Pacino, Dario

2017-01-01

The cargo-mix problem aims at selecting the amount of containers of a given type to load on a vessel. In this article we present an extended definition that includes the analysis of a circular route with draft restrictions, limitations on expected cargo and the use of a block stowage strategy...

46 CFR 308.545 - Facultative cargo policy, Form MA-316.

Science.gov (United States)

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Facultative cargo policy, Form MA-316. 308.545 Section 308.545 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK... policy, Form MA-316. The standard form of War Risk Facultative Cargo Policy, Form MA-316, may be obtained...

Reducing Mission Logistics with Multipurpose Cargo Transfer Bags

Science.gov (United States)

Baccus, Shelley; Broyan, James Lee, Jr.; Borrego, Melissa

2016-01-01

The Logistics Reduction (LR) project within Advanced Exploration Systems (AES) is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag (CTB), the common logistics carrying bag for the International Space Station (ISS). After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unfolded into a flat panel for reuse. Concepts and potential benefits for various MCTB applications will be discussed including partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing. Acoustic MCTBs are currently in use on ISS to reduce the noise generated by the T2 treadmill, which reaches the hazard limit at high speeds. The development of the AMCTB included identification of keep-out zones, acoustic properties, deployment considerations, and structural testing. Features developed for these considerations are applicable to MCTBs for all crew outfitting applications.

Shared Value Potential of Transporting Cargo via Hyperloop

Directory of Open Access Journals (Sweden)

Max Werner

2016-08-01

Full Text Available This research estimates the shared value created by constructing a hypothetical Hyperloop to transport cargo along 300 km in Northern Germany. Following Porter-Kramer (2011, we identified and evaluated eight factors that create shared value: travel speed, operating costs, safety, noise pollution, air pollution, climate effect/carbon footprint, separation effect/ property efficiency, and maintenance. Using official data compiled by several German institutes and organizations, we conducted comparative analysis to quantify and compare the abovementioned factors for Hyperloop and over-the-road cargo transport in Germany. Then, we monetized the individual and collective benefits of the shared value created by Hyperloop replacing a significant share of cargo transported by truck. Our findings indicate that the hypothetical Hyperloop project in Northern Germany would create €660 to €900 million of shared value annually. Our research method establishes a framework for assessing future transportation projects like Hyperloop, and our findings can be generalized to industrialized nations beyond Germany.

How to Steer and Lead Educational Processes in a Digital Medium Environment?

DEFF Research Database (Denmark)

Tække, Jesper

This paper is about challenges to steering and leadership of educational interaction in classrooms provided by the new medium environment that comes with digital media. In the new medium environment, the old way of steering what is going on in the classroom appears not to work any more since...

46 CFR 91.60-15 - Cargo Ship Safety Radio Certificate.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Cargo Ship Safety Radio Certificate. 91.60-15 Section 91... VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 91.60-15 Cargo Ship Safety Radio Certificate. Every vessel equipped with a radio installation on...

Field test of wake steering at an offshore wind farm

Directory of Open Access Journals (Sweden)

P. Fleming

2017-05-01

Full Text Available In this paper, a field test of wake-steering control is presented. The field test is the result of a collaboration between the National Renewable Energy Laboratory (NREL and Envision Energy, a smart energy management company and turbine manufacturer. In the campaign, an array of turbines within an operating commercial offshore wind farm in China have the normal yaw controller modified to implement wake steering according to a yaw control strategy. The strategy was designed using NREL wind farm models, including a computational fluid dynamics model, Simulator fOr Wind Farm Applications (SOWFA, for understanding wake dynamics and an engineering model, FLOw Redirection and Induction in Steady State (FLORIS, for yaw control optimization. Results indicate that, within the certainty afforded by the data, the wake-steering controller was successful in increasing power capture, by amounts similar to those predicted from the models.

Does pedalling on a recumbent bicycle influence the cyclist’s steering behaviour?

NARCIS (Netherlands)

Boon, K.M.; Klap, P.; Van Lanen, J.A.; Letsoin, G.J.; Jansen, A.J.

2014-01-01

The paper presents the answer to the question how pedalling on a specific recumbent bicycle, such as the VeloX3, influences a cyclist’s ability to steer the bicycle. The research aims to find the correlation between pedalling and the undesired steering movements it creates. To test this assumption a

Power unit-cargo space link in transport

Directory of Open Access Journals (Sweden)

Radmilović Zoran R.

2005-01-01

Full Text Available This paper deals with transportation technology regarding links between power unit and cargo space. These links can be divided into two groups: rigid and flexible. Rigid link, established between power unit and cargo space, is dominant in maritime and road transport (sea ships and trucks, and occasionally in transport on inland waterways (self- propelled barges. Flexible link is used in the railroad transport (systems with trailers and semi trailers, and in inland waterway transport (push - towing and pulling systems, and combinations of the systems. The main goal of this research is determination of possible link types and organization of the means of transportation.

Steering characteristic of an articulated bus under quasi steady maneuvering

Science.gov (United States)

Ubaidillah, Setiawan, Budi Agus; Aridharma, Airlangga Putra; Lenggana, Bhre Wangsa; Caesar, Bernardus Placenta Previo

2018-02-01

Articulated buses have been being preferred as public transportation modes due to their operational capacity. Therefore, passenger safety must be the priority of this public service vehicle. This research focused on the analytical approach of steering characteristics of an articulated bus when it maneuvered steadily. Such turning condition could be referred as a stability parameter of the bus for preliminary handling assessment. The analytical approach employed kinematics relationship between front and rear bodies as well as steering capabilities. The quasi steady model was developed to determine steering parameters such as turning radius, oversteer, and understeer. The mathematical model was useful for determining both coefficients of understeer and oversteer. The dimension of articulated bus followed a commonly used bus as utilized in Trans Jakarta busses. Based on the simulation, for one minimum center of the body, the turning radius was calculated about 8.8 m and 7.6 m at steady turning speed of 10 km/h. In neutral condition, the minimum road radius should be 6.5 m at 10 km/h and 6.9 m at 40 km/h. For two centers of the body and oversteer condition, the front body has the turning radius of 8.8 m, while, the rear body has the turning radius of 9.8 m at both turning speeds of 40 km/h. The other steering parameters were discussed accordingly.

Behavioral characteristics of Hanwoo ( steers at different growth stages and seasons

Directory of Open Access Journals (Sweden)

Na Yeon Kim

2017-10-01

Full Text Available Objective This research analyzed behavioral characteristics of Hanwoo (Bos taurus coreanae steers during each season and growth stage to enable measurement of the animals’ welfare level for precision livestock farming. Methods A hundred-eight beef steers were divided into three equal groups at a Hanwoo farm according to their growth stage: growing stage (GS, 8 months; early-fattening stage (EFS, 19 months; and late-fattening stage (LFS, 30 months. Twelve behavioral categories were continuously recorded for 13 day-time hours in each four seasons with three replications. Results Time spent standing was found to be significantly longer in summer at all growth stages (p<0.05. Hanwoos at the GS spent significantly longer standing time in spring and summer than those at the EFS and LFS (p<0.05. Lying time in summer was the shortest for all growth stages (p<0.05. Steers at the LFS spent significantly longer lying time than that at the GS (p<0.05 in summer. For GS and EFS, time spent eating in spring and autumn were longer than in summer and winter (p<0.05. Eating time was the longest for the GS in spring, autumn, and winter, excluding for the LFS in winter (p<0.05. Regarding ruminating, steers at the LFS spent significantly shorter time than those at other stages in all seasons (p<0.05. GS and EFS steers showed the longest walking time in summer compared with other seasons (p<0.05. At GS and LFS, drinking time in summer was the longest of all seasons (p<0.05. Sleeping time was significantly shorter in summer compared with the other seasons (p<0.05. Self-grooming time was the longest in winter for all growth stages (p<0.05. Conclusion Steers were found to have more variable behavioral patterns during summer and the GS and less active behaviors during the LFS, thus extra care seems necessary during the GS, LFS, and summer period.

Steering of Educational Processes in a Digital Medium Environment

DEFF Research Database (Denmark)

Tække, Jesper; Paulsen, Michael

2015-01-01

by systems theory we outline a more adequate way of teaching in the new medium environment – a teaching that can manage the new situation and use the new possibilities provided by the digital media. The argumentation builds on empirical findings from the action research project Socio Media Education (SME......This paper is about challenges to steering and leadership of educational interaction in classrooms provided by the new medium environment that comes with digital media. In the new medium environment, the old way of steering what is going on in the classroom appears not to work since...

Experimental ship fire measurements with simulated radioactive cargo

International Nuclear Information System (INIS)

Koski, J.A.; Arviso, M.; Bobbe, J.G.; Wix, S.D.; Cole, J.K.; Hohnstreiter, G.F.; Beene, D.E. Jr.

1997-10-01

Results from a series of eight test fires ranging in size from 2.2 to 18.8 MW conducted aboard the Coast Guard fire test ship Mayo Lykes at Mobile, Alabama are presented and discussed. Tests aboard the break bulk type cargo ship consisted of heptane spray fires simulating engine room and galley fires, wood crib fires simulating cargo hold fires, and pool fires staged for comparison to land based regulatory fire results. Primary instrumentation for the tests consisted of two pipe calorimeters that simulated a typical package shape for radioactive materials packages

Experimental ship fire measurements with simulated radioactive cargo

International Nuclear Information System (INIS)

Koski, J.A.; Arvisol, M.; Bobbe, J.G.; Wix, S.D.; Cole, J.K.; Hohnstreiter, G.F.; Wix, S.D.; Beene, D.E.; Keane, M.P.

1998-01-01

Results from a series of eight test fires ranging in size from 2.2 to 18.8 MW conducted aboard the Coast Guard fire test ship Mayo Lykes at Mobile, Alabama are presented and discussed. Tests aboard the break-bulk type cargo ship consisted of heptane spray fires simulating engine room and galley fires, wood crib fires simulating cargo hold fires, and pool fires staged for comparison to land-based regulatory fire results. Primary instrumentation for the tests consisted of two pipe calorimeters that simulated a typical package shape for radioactive materials packages. (authors)

Real-Time Application Performance Steering and Adaptive Control

National Research Council Canada - National Science Library

Reed, Daniel

2002-01-01

.... The objective of the Real-time Application Performance Steering and Adaptive Control project is to replace ad hoc, post-mortem performance optimization with an extensible, portable, and distributed...

Design of heavy lift cargo aircraft

Data.gov (United States)

National Aeronautics and Space Administration — This is the bird of the skies of the future. The heavy lift cargo aircraft which is currently being developed by me has twice the payload capacity of an Antonov...

National Security Science and Technology Initiative: Air Cargo Screening

Energy Technology Data Exchange (ETDEWEB)

Bingham, Philip R [ORNL; White, Tim [Pacific Northwest National Laboratory (PNNL); Cespedes, Ernesto [Idaho National Laboratory (INL); Bowerman, Biays [Brookhaven National Laboratory (BNL); Bush, John [Battelle

2010-11-01

The non-intrusive inspection (NII) of consolidated air cargo carried on commercial passenger aircraft continues to be a technically challenging, high-priority requirement of the Department of Homeland Security's Science and Technology Directorate (DHS S&T), the Transportation Security Agency and the Federal Aviation Administration. The goal of deploying a screening system that can reliably and cost-effectively detect explosive threats in consolidated cargo without adversely affecting the flow of commerce will require significant technical advances that will take years to develop. To address this critical National Security need, the Battelle Memorial Institute (Battelle), under a Cooperative Research and Development Agreement (CRADA) with four of its associated US Department of Energy (DOE) National Laboratories (Oak Ridge, Pacific Northwest, Idaho, and Brookhaven), conducted a research and development initiative focused on identifying, evaluating, and integrating technologies for screening consolidated air cargo for the presence of explosive threats. Battelle invested $8.5M of internal research and development funds during fiscal years 2007 through 2009. The primary results of this effort are described in this document and can be summarized as follows: (1) Completed a gap analysis that identified threat signatures and observables, candidate technologies for detection, their current state of development, and provided recommendations for improvements to meet air cargo screening requirements. (2) Defined a Commodity/Threat/Detection matrix that focuses modeling and experimental efforts, identifies technology gaps and game-changing opportunities, and provides a means of summarizing current and emerging capabilities. (3) Defined key properties (e.g., elemental composition, average density, effective atomic weight) for basic commodity and explosive benchmarks, developed virtual models of the physical distributions (pallets) of three commodity types and three

A Parallel and Distributed Surrogate Model Implementation for Computational Steering

KAUST Repository

Butnaru, Daniel

2012-06-01

Understanding the influence of multiple parameters in a complex simulation setting is a difficult task. In the ideal case, the scientist can freely steer such a simulation and is immediately presented with the results for a certain configuration of the input parameters. Such an exploration process is however not possible if the simulation is computationally too expensive. For these cases we present in this paper a scalable computational steering approach utilizing a fast surrogate model as substitute for the time-consuming simulation. The surrogate model we propose is based on the sparse grid technique, and we identify the main computational tasks associated with its evaluation and its extension. We further show how distributed data management combined with the specific use of accelerators allows us to approximate and deliver simulation results to a high-resolution visualization system in real-time. This significantly enhances the steering workflow and facilitates the interactive exploration of large datasets. © 2012 IEEE.

Cargo container inspection test program at ARPA's Nonintrusive Inspection Technology Testbed

Science.gov (United States)

Volberding, Roy W.; Khan, Siraj M.

1994-10-01

An x-ray-based cargo inspection system test program is being conducted at the Advanced Research Project Agency (ARPA)-sponsored Nonintrusive Inspection Technology Testbed (NITT) located in the Port of Tacoma, Washington. The test program seeks to determine the performance that can be expected from a dual, high-energy x-ray cargo inspection system when inspecting ISO cargo containers. This paper describes an intensive, three-month, system test involving two independent test groups, one representing the criminal smuggling element and the other representing the law enforcement community. The first group, the `Red Team', prepares ISO containers for inspection at an off-site facility. An algorithm randomly selects and indicates the positions and preparation of cargoes within a container. The prepared container is dispatched to the NITT for inspection by the `Blue Team'. After in-gate processing, it is queued for examination. The Blue Team inspects the container and decides whether or not to pass the container. The shipment undergoes out-gate processing and returns to the Red Team. The results of the inspection are recorded for subsequent analysis. The test process, including its governing protocol, the cargoes, container preparation, the examination and results available at the time of submission are presented.

Multi-Layer Traffic Steering

DEFF Research Database (Denmark)

Fotiadis, Panagiotis; Polignano, Michele; Gimenez, Lucas Chavarria

2013-01-01

This paper investigates the potentials of traffic steering in the Radio Resource Control (RRC) Idle state by evaluating the Absolute Priorities (AP) framework in a multilayer Long Term Evolution (LTE) macrocell scenario. Frequency priorities are broadcast on the system information and RRC Idle...... periods are not significantly long. Finally, better alignment between the RRC Connected and Idle mobility procedures is observed, guarantying significant decrease of handovers/reselections and potential battery life savings by minimizing the Inter-Frequency (IF) measurement rate in the RRC Idle....

Safety evaluation for packaging transport of LSA-II liquids in MC-312 cargo tanks

Energy Technology Data Exchange (ETDEWEB)

Carlstrom, R.F.

1996-09-11

This safety evaluation for packaging authorizes the onsite transfer of bulk LSA-II radioactive liquids in the 222-S Laboratory Cargo Tank and Liquid Effluent Treatment Facility Cargo Tanks (which are U.S. Department of Transportation MC-312 specification cargo tanks) from their operating facilities to tank farm facilities.

Progress on the ITER H&CD EC upper launcher steering-mirror control system

NARCIS (Netherlands)

Collazos, A.; Bertizzolo, R.; Chavan, R.; Dolizy, F.; Felici, F.; Goodman, T.P.; Henderson, M.A.; Landis, J.-D.; Sanchez, F.

2010-01-01

The ITER Heating and Current Drive Upper Launcher (H&CD EC UL) uses a pneumomechanical steering-mirror assembly (SMA) to steer the RF beams for their deposition in the appropriate location in the plasma to control magnetohydrodynamic activity (neoclassical tearing modes (NTMs) and sawtooth

46 CFR 105.25-7 - Ventilation systems for cargo tank or pumping system compartment.

Science.gov (United States)

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Ventilation systems for cargo tank or pumping system... Requirements-When Cargo Tanks Are Installed Below Decks § 105.25-7 Ventilation systems for cargo tank or pumping system compartment. (a) Each compartment shall be provided with a mechanical exhaust system...

Modulation of the acute phase response in feedlot steers supplemented with Saccharomyces cerevisiae

Science.gov (United States)

This study was designed to determine the effect of supplementing feedlot steers with Saccharomyces cerevisiae CNCM I-1079 (SC) on the acute phase response to a lipopolysaccharide (LPS) challenge. Steers (n = 18; 266 ± 4 kilograms body weight) were separated into three treatment groups (n = 6/treatm...

Three-bead steering microswimmers

Science.gov (United States)

Rizvi, Mohd Suhail; Farutin, Alexander; Misbah, Chaouqi

2018-02-01

The self-propelled microswimmers have recently attracted considerable attention as model systems for biological cell migration as well as artificial micromachines. A simple and well-studied microswimmer model consists of three identical spherical beads joined by two springs in a linear fashion with active oscillatory forces being applied on the beads to generate self-propulsion. We have extended this linear microswimmer configuration to a triangular geometry where the three beads are connected by three identical springs in an equilateral triangular manner. The active forces acting on each spring can lead to autonomous steering motion; i.e., allowing the swimmer to move along arbitrary paths. We explore the microswimmer dynamics analytically and pinpoint its rich character depending on the nature of the active forces. The microswimmers can translate along a straight trajectory, rotate at a fixed location, as well as perform a simultaneous translation and rotation resulting in complex curved trajectories. The sinusoidal active forces on the three springs of the microswimmer contain naturally four operating parameters which are more than required for the steering motion. We identify the minimal operating parameters which are essential for the motion of the microswimmer along any given arbitrary trajectory. Therefore, along with providing insights into the mechanics of the complex motion of the natural and artificial microswimmers, the triangular three-bead microswimmer can be utilized as a model for targeted drug delivery systems and autonomous underwater vehicles where intricate trajectories are involved.

Active Return-to-Center Control Based on Torque and Angle Sensors for Electric Power Steering Systems.

Science.gov (United States)

Du, Pan-Pan; Su, Hao; Tang, Gong-You

2018-03-14

This paper presents a complete control strategy of the active return-to-center (RTC) control for electric power steering (EPS) systems. We first establish the mathematical model of the EPS system and analyze the source and influence of the self-aligning torque (SAT). Second, based on the feedback signals of steering column torque and steering wheel angle, we give the trigger conditions of a state switch between the steering assist state and the RTC state. In order to avoid the sudden change of the output torque for the driving motor when the state switches frequently between the steering assist state and the RTC state, we design an undisturbed state switching logic algorithm. This state switching logic algorithm ensures that the output value of the RTC controller is set to an initial value and increases in given steps up to a maximum value after entering the RTC state, and the output value of the RTC controller will reduce in given steps down to zero when exiting the RTC state. This therefore ensures smooth switch control between the two states and improves the driver's steering feeling. Third, we design the RTC controller, which depends upon the feedback signals of the steering wheel angle and the angular velocity. In addition, the controller increases the auxiliary control function of the RTC torque based on vehicle speed. The experimental results show that the active RTC control method does not affect the basic assist characteristics, which effectively reduces the residual angle of the steering wheel at low vehicle speed and improves the RTC performance of the vehicle.

Experimental nonlocal steering of Bohmian trajectories.

Science.gov (United States)

Xiao, Ya; Kedem, Yaron; Xu, Jin-Shi; Li, Chuan-Feng; Guo, Guang-Can

2017-06-26

Interpretations of quantum mechanics (QM), or proposals for underlying theories, that attempt to present a definite realist picture, such as Bohmian mechanics, require strong non-local effects. Naively, these effects would violate causality and contradict special relativity. However if the theory agrees with QM the violation cannot be observed directly. Here, we demonstrate experimentally such an effect: we steer the velocity and trajectory of a Bohmian particle using a remote measurement. We use a pair of photons and entangle the spatial transverse position of one with the polarization of the other. The first photon is sent to a double-slit-like apparatus, where its trajectory is measured using the technique of Weak Measurements. The other photon is projected to a linear polarization state. The choice of polarization state, and the result, steer the first photon in the most intuitive sense of the word. The effect is indeed shown to be dramatic, while being easy to visualize. We discuss its strength and what are the conditions for it to occur.

46 CFR Table I to Part 150 - Alphabetical List of Cargoes

Science.gov (United States)

2010-10-01

... cargo name), see Caustic potash solution 5 2 CPS Potassium oleate 34 POE Potassium salt of polyolefin... Sodium polyacrylate solution 43 2 Sodium salt of Ferric hydroxyethylethylenediaminetriacetic acid... 46 Shipping 5 2010-10-01 2010-10-01 false Alphabetical List of Cargoes I Table I to Part 150...

Using Shape Memory Alloys: A Dynamic Data Driven Approach

KAUST Repository

Douglas, Craig C.

2013-06-01

Shape Memory Alloys (SMAs) are capable of changing their crystallographic structure due to changes of either stress or temperature. SMAs are used in a number of aerospace devices and are required in some devices in exotic environments. We are developing dynamic data driven application system (DDDAS) tools to monitor and change SMAs in real time for delivering payloads by aerospace vehicles. We must be able to turn on and off the sensors and heating units, change the stress on the SMA, monitor on-line data streams, change scales based on incoming data, and control what type of data is generated. The application must have the capability to be run and steered remotely as an unmanned feedback control loop.

Einstein-Podolsky-Rosen steering: Its geometric quantification and witness

Science.gov (United States)

Ku, Huan-Yu; Chen, Shin-Liang; Budroni, Costantino; Miranowicz, Adam; Chen, Yueh-Nan; Nori, Franco

2018-02-01

We propose a measure of quantum steerability, namely, a convex steering monotone, based on the trace distance between a given assemblage and its corresponding closest assemblage admitting a local-hidden-state (LHS) model. We provide methods to estimate such a quantity, via lower and upper bounds, based on semidefinite programming. One of these upper bounds has a clear geometrical interpretation as a linear function of rescaled Euclidean distances in the Bloch sphere between the normalized quantum states of (i) a given assemblage and (ii) an LHS assemblage. For a qubit-qubit quantum state, these ideas also allow us to visualize various steerability properties of the state in the Bloch sphere via the so-called LHS surface. In particular, some steerability properties can be obtained by comparing such an LHS surface with a corresponding quantum steering ellipsoid. Thus, we propose a witness of steerability corresponding to the difference of the volumes enclosed by these two surfaces. This witness (which reveals the steerability of a quantum state) enables one to find an optimal measurement basis, which can then be used to determine the proposed steering monotone (which describes the steerability of an assemblage) optimized over all mutually unbiased bases.

Design of a steering stabilizer based on CAN bus

Science.gov (United States)

Zhan, Zhaomin; Yan, Yibin

2018-04-01

This design realizes a posture correction device of griping steering wheel based on CAN bus, which is embedded in the steering wheel of vehicles. The system aims to detect the drivers' abnormal griping postures and provides drivers with classification alerts, by combining the recorded griping postures data and the vehicle speed data that are obtained via the CAN bus. The warning information are automatically stored and retained in the device for 12 months. To enhance the alerting effect, the count of this warning message for both the latest month and the last 12 months are displayed on the dashboard panel. In addition to prevent itself from being blocked and self-detect any faults in advance, the appliance also provide a self-test function, which will communicate with the integrated instrument system in vehicle and do simulation test right after the vehicle power on. This appliance can help to urge and ensure drivers to operate the steering wheel correctly, effectively, and timely; prevent some typical incorrect behaviors which commonly happen along with the change of griping postures, such as the using cellphone, and ultimately, reduce the incidence of traffic accidents.

Study on Alternative Cargo Launch Options from the Lunar Surface

Energy Technology Data Exchange (ETDEWEB)

Cheryl A. Blomberg; Zamir A. Zulkefli; Spencer W. Rich; Steven D. Howe

2013-07-01

In the future, there will be a need for constant cargo launches from Earth to Mars in order to build, and then sustain, a Martian base. Currently, chemical rockets are used for space launches. These are expensive and heavy due to the amount of necessary propellant. Nuclear thermal rockets (NTRs) are the next step in rocket design. Another alternative is to create a launcher on the lunar surface that uses magnetic levitation to launch cargo to Mars in order to minimize the amount of necessary propellant per mission. This paper investigates using nuclear power for six different cargo launching alternatives, as well as the orbital mechanics involved in launching cargo to a Martian base from the moon. Each alternative is compared to the other alternative launchers, as well as compared to using an NTR instead. This comparison is done on the basis of mass that must be shipped from Earth, the amount of necessary propellant, and the number of equivalent NTR launches. Of the options, a lunar coil launcher had a ship mass that is 12.7% less than the next best option and 17 NTR equivalent launches, making it the best of the presented six options.

46 CFR 308.524 - Application for cancellation of Open Cargo Policy, Form MA-304.

Science.gov (United States)

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Application for cancellation of Open Cargo Policy, Form... § 308.524 Application for cancellation of Open Cargo Policy, Form MA-304. The standard form of application for cancellation of an Open Cargo Policy Form MA-304 may be obtained from the American War Risk...

Stereo imaging and random array stratified imaging for cargo radiation inspecting

International Nuclear Information System (INIS)

Wang Jingjin; Zeng Yu

2003-01-01

This paper presents a Stereo Imaging and Random Array Stratified Imaging for cargo container radiation Inspecting. By using dual-line vertical detector array scan, a stereo image of inspected cargo can be obtained and watched with virtual reality view. The random detector array has only one-row of detectors but distributed in a certain horizontal dimension randomly. To scan a cargo container with this random array detector, a 'defocused' image is obtained. By using 'anti-random focusing', one layer of the image can be focused on the background of all defocused images from other layers. A stratified X-ray image of overlapped bike wheels is presented

Studies of steered arc motion and macroparticle production in PVD processing

International Nuclear Information System (INIS)

Craven, A.L.

2000-03-01

During the past decade the production industry has constantly strived to improve performance and cut costs, this has been aided by the development of high performance tools. The advancement of these tools has been accomplished by the application of hard wearing, low friction, coatings. A key process in the production of such coatings is Physical Vapour Deposition (PVD). Interest in such thin films has led to much research effort, both academic and industrial, being devoted to the area. In order that these advancements in technology continue, research into the fundamental aspects of PVD is required. This thesis describes research and experimental studies which have been performed to study the effect of 'steering' an electric arc on various aspects of its behaviour. 'Steering' of the arc is achieved by applying external magnetic fields which allow the guidance of the path of the arc. Work by earlier authors has aimed to control the arc more fully. The research presented here is based of a novel electromagnetic three coil steering array of cylindrical geometry. With such coils it is possible to vary the field profiles to a greater degree than has been previously achieved, permitting a greater range of steering arrangements/fields to be applied. The research presented is divided into two distinct areas: Firstly a number of experiments were performed to assess the effectiveness of the new steering coils on the motion of the arc. A personal computer was used here along with new arc motion monitoring electronics. This enabled the simultaneous measurement of the orbital transit times and also the degree of travel perpendicular to the steered direction of motion of the arc, as it traversed the surface of the cathode. Such information was then used to produce values for standard deviation of the arc from its steered path, velocity of the arc and a diffusion constant related to the motion of the are. Such values then allowed evaluation of the stochastic model of arc motion

46 CFR 308.523 - Application for revision of Open Cargo Policy, Form MA-303.

Science.gov (United States)

2010-10-01

... 46 Shipping 8 2010-10-01 2010-10-01 false Application for revision of Open Cargo Policy, Form MA... Application for revision of Open Cargo Policy, Form MA-303. An application for the revision of an Open Cargo Policy shall be filed in duplicate with the Underwriting Agent on a form which may be obtained from the...

Current steering with partial tripolar stimulation mode in cochlear implants.

Science.gov (United States)

Wu, Ching-Chih; Luo, Xin

2013-04-01

The large spread of excitation is a major cause of poor spectral resolution for cochlear implant (CI) users. Partial tripolar (pTP) mode has been proposed to reduce current spread by returning an equally distributed fraction (0.5 × σ) of current to two flanking electrodes and the rest to an extra-cochlear ground. This study tested the efficacy of incorporating current steering into pTP mode to add spectral channels. Different proportions of current [α × σ and (1 - α) × σ] were returned to the basal and apical flanking electrodes respectively to shape the electric field. Loudness and pitch perception with α from 0 to 1 in steps of 0.1 was simulated with a computational model of CI stimulation and tested on the apical, middle, and basal electrodes of six CI subjects. The highest σ allowing for full loudness growth within the implant compliance limit was chosen for each main electrode. Pitch ranking was measured between pairs of loudness-balanced steered pTP stimuli with an α interval of 0.1 at the most comfortable level. Results demonstrated that steered pTP stimuli with α around 0.5 required more current to achieve equal loudness than those with α around 0 or 1, maybe due to more focused excitation patterns. Subjects usually perceived decreasing pitches as α increased from 0 to 1, somewhat consistent with the apical shift of the center of gravity of excitation pattern in the model. Pitch discrimination was not better with α around 0.5 than with α around 0 or 1, except for some subjects and electrodes. For three subjects with better pitch discrimination, about half of the pitch ranges of two adjacent main electrodes overlapped with each other in steered pTP mode. These results suggest that current steering with focused pTP mode may improve spectral resolution and pitch perception with CIs.

CCCT - NCTN Steering Committees - Pediatric and Adolescent Tumor

Science.gov (United States)

The Pediatric and Adolescent Solid Tumor Steering Committee addresses the design, prioritization and evaluation of concepts for large phase 2 and phase 3 clinical trials in extracranial solid tumors of children and youth.

Carcass characteristics and meat quality of Hereford sired steers born to beef-cross-dairy and Angus breeding cows.

Science.gov (United States)

Coleman, Lucy W; Hickson, Rebecca E; Schreurs, Nicola M; Martin, Natalia P; Kenyon, Paul R; Lopez-Villalobos, Nicolas; Morris, Stephen T

2016-11-01

Steers from Angus, Angus×Holstein Friesian, Angus×Holstein Friesian-Jersey and Angus×Jersey cows and a Hereford sire were measured for their carcass and meat quality characteristics. Steers from the Angus×Holstein Friesian cows had a greater final body weight and carcass weight (P<0.05). Steers from Angus×Jersey cows had the lowest carcass weight and dressing-out percentage (P<0.05). There was a greater fat depth over the rump at 12 and 18months of age for the steers from Angus cows (P<0.05) but, not at 24months of age. The steers had similar meat quality characteristics across the breed groups. Steers from Angus×Holstein Friesian and Angus×Jersey cows had a higher ratio of n6 to n3 fatty acids. Using beef-cross-dairy cows to produce steers for meat production does not impact on meat quality. Using Jersey in the breed cross reduced the carcass tissues in the live weight and the potential meat yield. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Highly integrated optical phased arrays: photonic integrated circuits for optical beam shaping and beam steering

Directory of Open Access Journals (Sweden)

Heck Martijn J.R.

2016-06-01

Full Text Available Technologies for efficient generation and fast scanning of narrow free-space laser beams find major applications in three-dimensional (3D imaging and mapping, like Lidar for remote sensing and navigation, and secure free-space optical communications. The ultimate goal for such a system is to reduce its size, weight, and power consumption, so that it can be mounted on, e.g. drones and autonomous cars. Moreover, beam scanning should ideally be done at video frame rates, something that is beyond the capabilities of current opto-mechanical systems. Photonic integrated circuit (PIC technology holds the promise of achieving low-cost, compact, robust and energy-efficient complex optical systems. PICs integrate, for example, lasers, modulators, detectors, and filters on a single piece of semiconductor, typically silicon or indium phosphide, much like electronic integrated circuits. This technology is maturing fast, driven by high-bandwidth communications applications, and mature fabrication facilities. State-of-the-art commercial PICs integrate hundreds of elements, and the integration of thousands of elements has been shown in the laboratory. Over the last few years, there has been a considerable research effort to integrate beam steering systems on a PIC, and various beam steering demonstrators based on optical phased arrays have been realized. Arrays of up to thousands of coherent emitters, including their phase and amplitude control, have been integrated, and various applications have been explored. In this review paper, I will present an overview of the state of the art of this technology and its opportunities, illustrated by recent breakthroughs.

Highly integrated optical phased arrays: photonic integrated circuits for optical beam shaping and beam steering

Science.gov (United States)

Heck, Martijn J. R.

2017-01-01

Technologies for efficient generation and fast scanning of narrow free-space laser beams find major applications in three-dimensional (3D) imaging and mapping, like Lidar for remote sensing and navigation, and secure free-space optical communications. The ultimate goal for such a system is to reduce its size, weight, and power consumption, so that it can be mounted on, e.g. drones and autonomous cars. Moreover, beam scanning should ideally be done at video frame rates, something that is beyond the capabilities of current opto-mechanical systems. Photonic integrated circuit (PIC) technology holds the promise of achieving low-cost, compact, robust and energy-efficient complex optical systems. PICs integrate, for example, lasers, modulators, detectors, and filters on a single piece of semiconductor, typically silicon or indium phosphide, much like electronic integrated circuits. This technology is maturing fast, driven by high-bandwidth communications applications, and mature fabrication facilities. State-of-the-art commercial PICs integrate hundreds of elements, and the integration of thousands of elements has been shown in the laboratory. Over the last few years, there has been a considerable research effort to integrate beam steering systems on a PIC, and various beam steering demonstrators based on optical phased arrays have been realized. Arrays of up to thousands of coherent emitters, including their phase and amplitude control, have been integrated, and various applications have been explored. In this review paper, I will present an overview of the state of the art of this technology and its opportunities, illustrated by recent breakthroughs.

Creating virtual electrodes with 2D current steering

Science.gov (United States)

Spencer, Thomas C.; Fallon, James B.; Shivdasani, Mohit N.

2018-06-01

Objective. Current steering techniques have shown promise in retinal prostheses as a way to increase the number of distinct percepts elicitable without increasing the number of implanted electrodes. Previously, it has been shown that ‘virtual’ electrodes can be created between simultaneously stimulated electrode pairs, producing unique cortical response patterns. This study investigated whether virtual electrodes could be created using 2D current steering, and whether these virtual electrodes can produce cortical responses with predictable spatial characteristics. Approach. Normally-sighted eyes of seven adult anaesthetised cats were implanted with a 42-channel electrode array in the suprachoroidal space and multi-unit neural activity was recorded from the visual cortex. Stimuli were delivered to individual physical electrodes, or electrodes grouped into triangular, rectangular, and hexagonal arrangements. Varying proportions of charge were applied to each electrode in a group to ‘steer’ current and create virtual electrodes. The centroids of cortical responses to stimulation of virtual electrodes were compared to those evoked by stimulation of single physical electrodes. Main results. Responses to stimulation of groups of up to six electrodes with equal ratios of charge on each electrode resulted in cortical activation patterns that were similar to those elicited by the central physical electrode (centroids: RM ANOVA on ranks, p  >  0.05 neural spread: one-way ANOVA on Ranks, p  >  0.05). We were also able to steer the centroid of activation towards the direction of any of the electrodes of the group by applying a greater charge to that electrode, but the movement in the centroid was not found to be significant. Significance. The results suggest that current steering is possible in two dimensions between up to at least six electrodes, indicating it may be possible to increase the number of percepts in patients without increasing the number

Active Return-to-Center Control Based on Torque and Angle Sensors for Electric Power Steering Systems

Directory of Open Access Journals (Sweden)

Pan-Pan Du

2018-03-01

Full Text Available This paper presents a complete control strategy of the active return-to-center (RTC control for electric power steering (EPS systems. We first establish the mathematical model of the EPS system and analyze the source and influence of the self-aligning torque (SAT. Second, based on the feedback signals of steering column torque and steering wheel angle, we give the trigger conditions of a state switch between the steering assist state and the RTC state. In order to avoid the sudden change of the output torque for the driving motor when the state switches frequently between the steering assist state and the RTC state, we design an undisturbed state switching logic algorithm. This state switching logic algorithm ensures that the output value of the RTC controller is set to an initial value and increases in given steps up to a maximum value after entering the RTC state, and the output value of the RTC controller will reduce in given steps down to zero when exiting the RTC state. This therefore ensures smooth switch control between the two states and improves the driver’s steering feeling. Third, we design the RTC controller, which depends upon the feedback signals of the steering wheel angle and the angular velocity. In addition, the controller increases the auxiliary control function of the RTC torque based on vehicle speed. The experimental results show that the active RTC control method does not affect the basic assist characteristics, which effectively reduces the residual angle of the steering wheel at low vehicle speed and improves the RTC performance of the vehicle.

Multipurpose Cargo Transfer Bags fro Reducing Exploration Mission Logistics

Science.gov (United States)

Baccus, Shelley; Broyan, James Lee, Jr.; Borrego, Melissa

2016-01-01

The Logistics Reduction (LR) project within the Advanced Exploration Systems (AES) division is tasked with reducing logistical mass and repurposing logistical items. Multipurpose Cargo Transfer Bags (MCTB) have been designed such that they can serve the same purpose as a Cargo Transfer Bag (CTB), the common logistics carrying bag for the International Space Station (ISS). After use as a cargo carrier, a regular CTB becomes trash, whereas the MCTB can be unfolded into a flat panel for reuse. Concepts and potential benefits for various MCTB applications will be discussed including partitions, crew quarters, solar radiation storm shelters, acoustic blankets, and forward osmosis water processing. Acoustic MCTBs are currently in use on ISS to reduce the noise generated by the T2 treadmill, which reaches the hazard limit at high speeds. The development of the AMCTB included identification of keep out zones, acoustic properties, deployment considerations, and structural testing. Features developed for these considerations are applicable to MCTBs for all crew outfitting applications.

Recent advances in fast neutron radiography for cargo inspection

International Nuclear Information System (INIS)

Sowerby, B.D.; Tickner, J.R.

2007-01-01

Fast neutron radiography techniques are attractive for screening cargo for contraband such as narcotics and explosives. Neutrons have the required penetration, they interact with matter in a manner complementary to X-rays and they can be used to determine elemental composition. Compared to neutron interrogation techniques that measure secondary radiation (neutron or gamma-rays), neutron radiography systems are much more efficient and rapid and they are much more amenable to imaging. However, for neutron techniques to be successfully applied to cargo screening, they must demonstrate significant advantages over well-established X-ray techniques. This paper reviews recent developments and applications of fast neutron radiography for cargo inspection. These developments include a fast neutron and gamma-ray radiography system that utilizes a 14 MeV neutron generator as well as fast neutron resonance radiography systems that use variable energy quasi-monoenergetic neutrons and pulsed broad energy neutron beams. These systems will be discussed and compared with particular emphasis on user requirements, sources, detector systems, imaging ability and performance

Adaptive Sliding Mode Control Method Based on Nonlinear Integral Sliding Surface for Agricultural Vehicle Steering Control

Directory of Open Access Journals (Sweden)

Taochang Li

2014-01-01

Full Text Available Automatic steering control is the key factor and essential condition in the realization of the automatic navigation control of agricultural vehicles. In order to get satisfactory steering control performance, an adaptive sliding mode control method based on a nonlinear integral sliding surface is proposed in this paper for agricultural vehicle steering control. First, the vehicle steering system is modeled as a second-order mathematic model; the system uncertainties and unmodeled dynamics as well as the external disturbances are regarded as the equivalent disturbances satisfying a certain boundary. Second, a transient process of the desired system response is constructed in each navigation control period. Based on the transient process, a nonlinear integral sliding surface is designed. Then the corresponding sliding mode control law is proposed to guarantee the fast response characteristics with no overshoot in the closed-loop steering control system. Meanwhile, the switching gain of sliding mode control is adaptively adjusted to alleviate the control input chattering by using the fuzzy control method. Finally, the effectiveness and the superiority of the proposed method are verified by a series of simulation and actual steering control experiments.

Detailed field test of yaw-based wake steering

DEFF Research Database (Denmark)

Fleming, P.; Churchfield, M.; Scholbrock, A.

2016-01-01

production. In the first phase, a nacelle-mounted scanning lidar was used to verify wake deflection of a misaligned turbine and calibrate wake deflection models. In the second phase, these models were used within a yaw controller to achieve a desired wake deflection. This paper details the experimental......This paper describes a detailed field-test campaign to investigate yaw-based wake steering. In yaw-based wake steering, an upstream turbine intentionally misaligns its yaw with respect to the inflow to deflect its wake away from a downstream turbine, with the goal of increasing total power...... design and setup. All data collected as part of this field experiment will be archived and made available to the public via the U.S. Department of Energy’s Atmosphere to Electrons Data Archive and Portal....

Adiabatic photo-steering theory in topological insulators

Science.gov (United States)

Inoue, Jun-ichi

2014-12-01

Feasible external control of material properties is a crucial issue in condensed matter physics. A new approach to achieving this aim, named adiabatic photo-steering, is reviewed. The core principle of this scheme is that several material constants are effectively turned into externally tunable variables by irradiation of monochromatic laser light. Two-dimensional topological insulators are selected as the optimal systems that exhibit a prominent change in their properties following the application of this method. Two specific examples of photo-steered quantum phenomena, which reflect topological aspects of the electronic systems at hand, are presented. One is the integer quantum Hall effect described by the Haldane model, and the other is the quantum spin Hall effect described by the Kane-Mele model. The topological quantities associated with these phenomena are the conventional Chern number and spin Chern number, respectively. A recent interesting idea, time-reversal symmetry breaking via a temporary periodic external stimulation, is also discussed.

Adiabatic photo-steering theory in topological insulators

International Nuclear Information System (INIS)

Inoue, Jun-ichi

2014-01-01

Feasible external control of material properties is a crucial issue in condensed matter physics. A new approach to achieving this aim, named adiabatic photo-steering, is reviewed. The core principle of this scheme is that several material constants are effectively turned into externally tunable variables by irradiation of monochromatic laser light. Two-dimensional topological insulators are selected as the optimal systems that exhibit a prominent change in their properties following the application of this method. Two specific examples of photo-steered quantum phenomena, which reflect topological aspects of the electronic systems at hand, are presented. One is the integer quantum Hall effect described by the Haldane model, and the other is the quantum spin Hall effect described by the Kane–Mele model. The topological quantities associated with these phenomena are the conventional Chern number and spin Chern number, respectively. A recent interesting idea, time-reversal symmetry breaking via a temporary periodic external stimulation, is also discussed. (focus issue review)

Cargo/Weapons Elevator Land Based Engineering Site

Data.gov (United States)

Federal Laboratory Consortium — The Cargo and Weapons Facility consists of a suite of full scale and component test facilities contiguously located in building 77H. The site was constructed in 1987...

Safety evaluation for packaging (Onsite) transport of LSA-II liquids in MC-312 cargo tanks

International Nuclear Information System (INIS)

Carlstrom, R.F.

1996-01-01

This safety evaluation for packaging authorizes the onsite transfer of bulk LSA-II radioactive liquids in the 222-S Laboratory Cargo Tank and Liquid Effluent Treatment Facility Cargo Tanks (which are U.S. Department of Transportation MC-312 specification cargo tanks) from their operating facilities to tank farm facilities

Multi-energy x-ray detectors to improve air-cargo security

Science.gov (United States)

Paulus, Caroline; Moulin, Vincent; Perion, Didier; Radisson, Patrick; Verger, Loïck

2017-05-01

X-ray based systems have been used for decades to screen luggage or cargo to detect illicit material. The advent of energy-sensitive photon-counting x-ray detectors mainly based on Cd(Zn)Te semi-conductor technology enables to improve discrimination between materials compared to single or dual energy technology. The presented work is part of the EUROSKY European project to develop a Single European Secure Air-Cargo Space. "Cargo" context implies the presence of relatively heavy objects and with potentially high atomic number. All the study is conducted on simulations with three different detectors: a typical dual energy sandwich detector, a realistic model of the commercial ME100 multi-energy detector marketed by MULTIX, and a ME100 "Cargo": a not yet existing modified multi-energy version of the ME100 more suited to air freight cargo inspection. Firstly, a comparison on simulated measurements shows the performances improvement of the new multi-energy detectors compared to the current dual-energy one. The relative performances are evaluated according to different criteria of separability or contrast-to-noise ratio and the impact of different parameters is studied (influence of channel number, type of materials and tube voltage). Secondly, performances of multi-energy detectors for overlaps processing in a dual-view system is accessed: the case of orthogonal projections has been studied, one giving dimensional values, the other one providing spectral data to assess effective atomic number. A method of overlap correction has been proposed and extended to multi-layer objects case. Therefore, Calibration and processing based on bi-material decomposition have been adapted for this purpose.

Mechanical design aspects of a "Long-Waveguide" version of the remote steering ECRH upper port launcher for ITER

NARCIS (Netherlands)

Ronden, D. M. S.; van den Berg, M.; Bongers, W. A.; Elzendoorn, B. S. Q.; M. F. Graswinckel,; Lamers, B.; Van Nigtevecht, K.; Verhoeven, A. G. A.; Henderson, M. A.

2008-01-01

The current status of the mechanical design of the remote steering electron cyclotron resonance heating upper port launching system for ITER is presented. Although an alternative front steering launcher has now been selected as the reference design for ITER, the development of a remote steering

Combining technologies - radiography and neutron based - for cargo security applications

International Nuclear Information System (INIS)

Gozani, T.; Liu, F.; Sivakumar, M.; Brown, D.

2004-01-01

Inspection of air and sea cargo has traditionally been done by X-ray systems of various energies relying on operators to analyze images looking for anomalies in the image of cargo that may signify a threat. This has shown only limited success in detecting explosives and other threats, which do not have any distinctive shapes. OSI Systems, through its subsidiaries Rapiscan and Ancore, has combined high-energy x-ray radiography with thermal neutron analysis (TNA) to create the combined system-''TNX''. The system provides automatic material specific detection of bulk threat items, like explosives, while furnishing the operator with a high-resolution image for weapons detection and also to identify anomalies for the TNA to inspect. Similarly the Pulsed Fast Neutron Analysis (PFNA) can be combined with high-energy x-ray to create a ''FNX'' system for both air and sea cargo applications. This enables the operator obtain a three dimensional image of the material composition of the cargo under inspection and remove the clutter from the image leaving only the potentially hazardous material(s) automatically while viewing a high resolution image for manifest verification and weapons. The current status of the technology will be discussed and data be presented

Speed-constrained three-axes attitude control using kinematic steering

Science.gov (United States)

Schaub, Hanspeter; Piggott, Scott

2018-06-01

Spacecraft attitude control solutions typically are torque-level algorithms that simultaneously control both the attitude and angular velocity tracking errors. In contrast, robotic control solutions are kinematic steering commands where rates are treated as the control variable, and a servo-tracking control subsystem is present to achieve the desired control rates. In this paper kinematic attitude steering controls are developed where an outer control loop establishes a desired angular response history to a tracking error, and an inner control loop tracks the commanded body angular rates. The overall stability relies on the separation principle of the inner and outer control loops which must have sufficiently different response time scales. The benefit is that the outer steering law response can be readily shaped to a desired behavior, such as limiting the approach angular velocity when a large tracking error is corrected. A Modified Rodrigues Parameters implementation is presented that smoothly saturates the speed response. A robust nonlinear body rate servo loop is developed which includes integral feedback. This approach provides a convenient modular framework that makes it simple to interchange outer and inner control loops to readily setup new control implementations. Numerical simulations illustrate the expected performance for an aggressive reorientation maneuver subject to an unknown external torque.

Scientific opinion on the evaluation of substances as acceptable previous cargoes for edible fats and oils

DEFF Research Database (Denmark)

Petersen, Annette

Shipping of edible fats and oils into Europe is permitted in bulk tanks, provided that the previous cargo is included in a positive list. The European Commission requested EFSA to evaluate the acceptability as previous cargoes for fats and oils the substances calcium lignosulphonate, methyl acetate...... the criteria for acceptability as previous cargoes. Due to uncertainties, mainly with regard to the composition and toxicity of the low molecular mass fraction, and the fact that the toxicological database is limited to the 40–65 grade and does not cover all grades of calcium lignosulphonate shipped...... as previous cargoes, the EFSA Panel on Contaminants in the Food Chain (CONTAM Panel) concluded that calcium lignosulphonate does not meet the criteria for acceptability as a previous cargo. Only food-grade ammonium sulphate meets the criteria for acceptability as a previous cargo due to uncertainties about...

Automated steering systems applied to complex horizontal well in South Italy

Energy Technology Data Exchange (ETDEWEB)

Calderoni, A.; Ligrone, A.; Trampini, A. [ENI AGIP (Italy); Oppelt, J.; Gauld, S. [Baker Hughes INTEQ (United States)

1998-12-31

A new drilling system that not only improved the quality of the borehole trajectory, but also resulted in significant savings in capital cost is described. The new concept in drilling is the result of a joint development between a major oil company and an oilfield service provider. Through providing a breakthrough technology in directional steering systems the accuracy of the borehole steering has been substantially improved. The new concept involves the use of a straight hole drilling system (SDD) in the upper hole section, in combination with the rotary steering system, AutoTrak, in the 8.5 inch hole size. By synergistically using the new automated tools, the well was drilled much more efficiently than offset wells, producing substantial time savings, a smoother trajectory and less friction. Details of the different automated directional drilling systems that were used together in a well for the first time at Monte Enoc 9 in South Italy are described. 2 refs., 3 tabs., 9 figs.

A Car-Steering Model Based on an Adaptive Neuro-Fuzzy Controller

Science.gov (United States)

Amor, Mohamed Anis Ben; Oda, Takeshi; Watanabe, Shigeyoshi

This paper is concerned with the development of a car-steering model for traffic simulation. Our focus in this paper is to propose a model of the steering behavior of a human driver for different driving scenarios. These scenarios are modeled in a unified framework using the idea of target position. The proposed approach deals with the driver’s approximation and decision-making mechanisms in tracking a target position by means of fuzzy set theory. The main novelty in this paper lies in the development of a learning algorithm that has the intention to imitate the driver’s self-learning from his driving experience and to mimic his maneuvers on the steering wheel, using linear networks as local approximators in the corresponding fuzzy areas. Results obtained from the simulation of an obstacle avoidance scenario show the capability of the model to carry out a human-like behavior with emphasis on learned skills.

46 CFR 35.30-10 - Cargo tank hatches, ullage holes, and Butterworth plates-TB/ALL.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo tank hatches, ullage holes, and Butterworth plates... OPERATIONS General Safety Rules § 35.30-10 Cargo tank hatches, ullage holes, and Butterworth plates—TB/ALL. No cargo tank hatches, ullage holes, or Butterworth plates shall be opened or shall remain open...

Light-field-driven currents in graphene

Science.gov (United States)

Higuchi, Takuya; Heide, Christian; Ullmann, Konrad; Weber, Heiko B.; Hommelhoff, Peter

2017-10-01

The ability to steer electrons using the strong electromagnetic field of light has opened up the possibility of controlling electron dynamics on the sub-femtosecond (less than 10-15 seconds) timescale. In dielectrics and semiconductors, various light-field-driven effects have been explored, including high-harmonic generation, sub-optical-cycle interband population transfer and the non-perturbative change of the transient polarizability. In contrast, much less is known about light-field-driven electron dynamics in narrow-bandgap systems or in conductors, in which screening due to free carriers or light absorption hinders the application of strong optical fields. Graphene is a promising platform with which to achieve light-field-driven control of electrons in a conducting material, because of its broadband and ultrafast optical response, weak screening and high damage threshold. Here we show that a current induced in monolayer graphene by two-cycle laser pulses is sensitive to the electric-field waveform, that is, to the exact shape of the optical carrier field of the pulse, which is controlled by the carrier-envelope phase, with a precision on the attosecond (10-18 seconds) timescale. Such a current, dependent on the carrier-envelope phase, shows a striking reversal of the direction of the current as a function of the driving field amplitude at about two volts per nanometre. This reversal indicates a transition of light-matter interaction from the weak-field (photon-driven) regime to the strong-field (light-field-driven) regime, where the intraband dynamics influence interband transitions. We show that in this strong-field regime the electron dynamics are governed by sub-optical-cycle Landau-Zener-Stückelberg interference, composed of coherent repeated Landau-Zener transitions on the femtosecond timescale. Furthermore, the influence of this sub-optical-cycle interference can be controlled with the laser polarization state. These coherent electron dynamics in

Ka-Band Electronically Steered CubeSat Antenna, Phase II

Data.gov (United States)

National Aeronautics and Space Administration — Kymeta Government Solutions (KGS) designed, analyzed, built, tested, and delivered a small, lightweight, low-cost, low-power electronically steered Ka-band prototype...

Design Optimization of Steering Mechanisms for Articulated Off-Road Vehicles Based on Genetic Algorithms

Directory of Open Access Journals (Sweden)

Chen Zhou

2018-02-01

Full Text Available Two cylinders arranged symmetrically on a frame have become a major form of steering mechanism for articulated off-road vehicles (AORVs. However, the differences of stroke and arm lead to pressure fluctuation, vibration noise, and a waste of torque. In this paper, the differences of stroke and arm are reduced based on a genetic algorithm (GA. First, the mathematical model of the steering mechanism is put forward. Then, the difference of stroke and arm are optimized using a GA. Finally, a FW50GLwheel loader is used as an example to demonstrate the proposed GA-based optimization method, and its effectiveness is verified by means of automatic dynamic analysis of mechanical systems (ADAMS. The stroke difference of the steering hydraulic cylinders was reduced by 92% and the arm difference reached a decrease of 78% through GA optimization, in comparison with unoptimized structures. The simulation result shows that the steering mechanism optimized by GA behaved better than by previous methods.

Evaluation of four steering wheels to determine driver hand placement in a static environment.

Science.gov (United States)

Mossey, Mary E; Xi, Yubin; McConomy, Shayne K; Brooks, Johnell O; Rosopa, Patrick J; Venhovens, Paul J

2014-07-01

While much research exists on occupant packaging both proprietary and in the literature, more detailed research regarding user preferences for subjective ratings of steering wheel designs is sparse in published literature. This study aimed to explore the driver interactions with production steering wheels in four vehicles by using anthropometric data, driver hand placement, and driver grip design preferences for Generation-Y and Baby Boomers. In this study, participants selected their preferred grip diameter, responded to a series of questions about the steering wheel grip as they sat in four vehicles, and rank ordered their preferred grip design. Thirty-two male participants (16 Baby Boomers between ages 47 and 65 and 16 Generation-Y between ages 18 and 29) participated in the study. Drivers demonstrated different gripping behavior between vehicles and between groups. Recommendations for future work in steering wheel grip design and naturalistic driver hand positioning are discussed. Copyright © 2014. Published by Elsevier Ltd.

Models for Comparing Air-Only and Sea/Air Transportation of Wartime Deployment Cargo

National Research Council Canada - National Science Library

Theres, Michael

1998-01-01

...) to an overseas Port of Debarkation (POD). This thesis evaluates a proposal to load air-transportable cargo aboard vessels at CONUS seaports and to ship that cargo to an appropriately located sea-air-interface (SAI...

Performance and welfare of steers housed on concrete slatted floors at fixed and dynamic (allometric based) space allowances.

Science.gov (United States)

Keane, Michael P; McGee, Mark; O'Riordan, Edward G; Kelly, Alan K; Earley, Bernadette

2018-04-03

The objectives of the study were to determine whether allometric equations are suitable for estimating the space requirements of finishing beef cattle housed on concrete slatted floors (CSF) and to examine the effect of fixed and dynamic space allowances on the performance and welfare of these cattle. Continental crossbred steers [n = 120: mean initial live weight, 590 (SD 29.8) kg] were blocked by breed, weight, and age and assigned to 1 of 5 space allowance treatments (3 fixed and 2 dynamic) on CSF: 1) 2.0 m2 per animal, 2) 2.5 m2 per animal, 3) 3.0 m2 per animal, 4) Equation 1 (E1); y = 0.033w0.667, where y = m2 per animal and w = body weight, and 5) Equation 2 (E2); y = 0.048w0.667. The length of the feed face was 3.0 m for all treatments. Steers were offered grass silage and concentrates ad libitum. DMI was recorded weekly on a pen basis. Steers were weighed and dirt scored every 14 d. Blood samples were collected every 28 d, and analyzed for complete cell counts. Behavior was recorded using closed-circuit infrared cameras. Steers' hooves were inspected for lesions at the beginning of the study and post-slaughter. Slaughter weight and ADG were lowest, and feed conversion ratio (FCR) was poorest, for steers accommodated at 2.0 m2, and slaughter weight and ADG were greatest, and FCR was the best, for steers accommodated at E2 (P 0.05) to those accommodated at 2.0 m2 and both 3.0 m2 and E1, whereas steers accommodated at 3.0 m2 and E1 were intermediate (P > 0.05) to 2.5 m2 and E2. Carcass weight of steers housed at 2.0 m2 was lower (P < 0.05) than all other treatments. Steers housed at 2.5 m2 had lower carcass weights (P < 0.05) than those with accommodated at E1 and E2, whereas the carcass weight of steers accommodated at 3.0 m2 was intermediate. Carcass fat scores and hide weights were lower (P < 0.05) in steers accommodated at 2.0 m2 than those housed at E2 with other treatments being intermediate. The number of steers lying at any one time and the number of

Displacement and force coupling control design for automotive active front steering system

Science.gov (United States)

Zhao, Wanzhong; Zhang, Han; Li, Yijun

2018-06-01

A displacement and force coupling control design for active front steering (AFS) system of vehicle is proposed in this paper. In order to investigate the displacement and force characteristics of the AFS system of the vehicle, the models of AFS system, vehicle, tire as well as the driver model are introduced. Then, considering the nonlinear characteristics of the tire force and external disturbance, a robust yaw rate control method is designed by applying a steering motor to generate an active steering angle to adjust the yaw stability of the vehicle. Based on mixed H2/H∞ control, the system robustness and yaw rate tracking performance are enforced by H∞ norm constraint and the control effort is captured through H2 norm. In addition, based on the AFS system, a planetary gear set and an assist motor are both added to realize the road feeling control in this paper to dismiss the influence of extra steering angle through a compensating method. Evaluation of the overall system is accomplished by simulations and experiments under various driving condition. The simulation and experiment results show the proposed control system has excellent tracking performance and road feeling performance, which can improve the cornering stability and maneuverability of vehicle.

Differential regulation of polarized synaptic vesicle trafficking and synapse stability in neural circuit rewiring in Caenorhabditis elegans.

Directory of Open Access Journals (Sweden)

Naina Kurup

2017-06-01

Full Text Available Neural circuits are dynamic, with activity-dependent changes in synapse density and connectivity peaking during different phases of animal development. In C. elegans, young larvae form mature motor circuits through a dramatic switch in GABAergic neuron connectivity, by concomitant elimination of existing synapses and formation of new synapses that are maintained throughout adulthood. We have previously shown that an increase in microtubule dynamics during motor circuit rewiring facilitates new synapse formation. Here, we further investigate cellular control of circuit rewiring through the analysis of mutants obtained in a forward genetic screen. Using live imaging, we characterize novel mutations that alter cargo binding in the dynein motor complex and enhance anterograde synaptic vesicle movement during remodeling, providing in vivo evidence for the tug-of-war between kinesin and dynein in fast axonal transport. We also find that a casein kinase homolog, TTBK-3, inhibits stabilization of nascent synapses in their new locations, a previously unexplored facet of structural plasticity of synapses. Our study delineates temporally distinct signaling pathways that are required for effective neural circuit refinement.

Investigating Einstein-Podolsky-Rosen steering of continuous-variable bipartite states by non-Gaussian pseudospin measurements

Science.gov (United States)

Xiang, Yu; Xu, Buqing; Mišta, Ladislav; Tufarelli, Tommaso; He, Qiongyi; Adesso, Gerardo

2017-10-01

Einstein-Podolsky-Rosen (EPR) steering is an asymmetric form of correlations which is intermediate between quantum entanglement and Bell nonlocality, and can be exploited as a resource for quantum communication with one untrusted party. In particular, steering of continuous-variable Gaussian states has been extensively studied theoretically and experimentally, as a fundamental manifestation of the EPR paradox. While most of these studies focused on quadrature measurements for steering detection, two recent works revealed that there exist Gaussian states which are only steerable by suitable non-Gaussian measurements. In this paper we perform a systematic investigation of EPR steering of bipartite Gaussian states by pseudospin measurements, complementing and extending previous findings. We first derive the density-matrix elements of two-mode squeezed thermal Gaussian states in the Fock basis, which may be of independent interest. We then use such a representation to investigate steering of these states as detected by a simple nonlinear criterion, based on second moments of the correlation matrix constructed from pseudospin operators. This analysis reveals previously unexplored regimes where non-Gaussian measurements are shown to be more effective than Gaussian ones to witness steering of Gaussian states in the presence of local noise. We further consider an alternative set of pseudospin observables, whose expectation value can be expressed more compactly in terms of Wigner functions for all two-mode Gaussian states. However, according to the adopted criterion, these observables are found to be always less sensitive than conventional Gaussian observables for steering detection. Finally, we investigate continuous-variable Werner states, which are non-Gaussian mixtures of Gaussian states, and find that pseudospin measurements are always more effective than Gaussian ones to reveal their steerability. Our results provide useful insights on the role of non

77 FR 11145 - Intent to Request Renewal From OMB of One Current Public Collection of Information: Air Cargo...

Science.gov (United States)

2012-02-24

... operating under a security program, and all-cargo carriers. The collections of information that make up this ICR are security programs, security threat assessments (STA), known shipper data via the Known Shipper Management System (KSMS), Air Cargo Data Management System (ACDMS), Cargo Reporting Tool for cargo screening...

Steering straight

Energy Technology Data Exchange (ETDEWEB)

Louie, Jacqueline

2011-12-15

Baker Hughes Inc. has developed a deep azimuthal resistivity measurement tool for subsurface navigation when drilling oil and gas wells. This tool, named AziTrak, has measurement-while-drilling and logging-while-drilling capabilities and data are sent in real time to the surface via mud pulse or wired pipe telemetry. This technology helps the operator detect and visualize bed boundaries in real time, thanks to 3D imagery and a 360 degree view of the subsurface. The AziTrak system makes it possible to steer proactively and to stay within the pay zone at all times to maximize production; the tool had excellent results in field applications. Although this tool is 5 times more expensive than conventional technologies, its use results in a more economic wellbore thanks to its great utility. If the operator puts a high degree of involvement into it, the AziTrak deep azimuthal resistivity measurement tool will allow him to stay in the pay zone at all times.

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons.

Science.gov (United States)

Niwa, Shinsuke

2017-10-18

Axonal transport and intraflagellar transport (IFT) are essential for axon and cilia morphogenesis and function. Kinesin superfamily proteins and dynein are molecular motors that regulate anterograde and retrograde transport, respectively. These motors use microtubule networks as rails. Caenorhabditis elegans (C. elegans) is a powerful model organism to study axonal transport and IFT in vivo. Here, I describe a protocol to observe axonal transport and IFT in living C. elegans. Transported cargo can be visualized by tagging cargo proteins using fluorescent proteins such as green fluorescent protein (GFP). C. elegans is transparent and GFP-tagged cargo proteins can be expressed in specific cells under cell-specific promoters. Living worms can be fixed by microbeads on 10% agarose gel without killing or anesthetizing the worms. Under these conditions, cargo movement can be directly observed in the axons and cilia of living C. elegans without dissection. This method can be applied to the observation of any cargo molecule in any cells by modifying the target proteins and/or the cells they are expressed in. Most basic proteins such as molecular motors and adaptor proteins that are involved in axonal transport and IFT are conserved in C. elegans. Compared to other model organisms, mutants can be obtained and maintained more easily in C. elegans. Combining this method with various C. elegans mutants can clarify the molecular mechanisms of axonal transport and IFT.

Evaluation of metal matrix composite to replace spheroidal graphite iron for a critical component, steering knuckle

International Nuclear Information System (INIS)

Vijayarangan, S.; Rajamanickam, N.; Sivananth, V.

2013-01-01

Highlights: ► A FE model is developed to study the suitability of MMC for steering knuckle. ► Structural analysis of steering knuckle is carried out for 12 load cases. ► The cross section of the critical region is optimized using genetic algorithm. ► The life of the MMC (Al-10 wt.% TiC) knuckle is compared before and after optimization. ► MMC material could replace SG iron for automotive steering knuckle. -- Abstract: Steering knuckle is considered as one of the critical component in automotive suspension system. It is subjected to time varying loads during its service life, leading to fatigue failure. Therefore, its design is an important aspect in the product development cycle. Currently, spheroidal graphite (SG) iron is widely used to manufacture steering knuckle in the commercial automobile sector. It has been observed from the knuckle manufacturers that advanced materials and weight reduction are the real need for the current automobile industry. Due to their high strength to weight ratio, Metal Matrix Composites (MMCs) have the potential to meet the demanded design requirements of the automotive industry, compared to conventional materials. In this work, an aluminum alloy reinforced with titanium carbide particulate is suggested as an alternate material in place of existing SG iron. Structural analysis of steering knuckle made of alternate material Al-10 wt.% TiC was performed using commercial code ANSYS. The results of steering knuckle made of MMC (Al-10 wt.% TiC) were compared with that of aluminum alloy and SG iron steering knuckles for its performance based on real time load cases. It is found from this analysis, the knuckle strut region has maximum stress and deflection during its life time. The critical strut region cross section area of knuckle was analyzed and geometrically optimized for minimum bending stress and deflection using genetic algorithm available in MatLab. Since, the knuckle experiences time varying loads, fatigue analysis also

19 CFR 4.33 - Diversion of cargo.

Science.gov (United States)

2010-04-01

... the result of a strike or other emergency at a United States port for which inward foreign cargo is... substituted for the domestic port of discharge. If the application is approved, it shall be handled in the...

Fixing Trailer Hitch for Roof Rack of Cargos

Directory of Open Access Journals (Sweden)

Darina Matisková

2017-02-01

Full Text Available This article presents a new technical solution of fixing trailer hitch which serves as a connection between single track vehicle and cargo, mainly light trailer. They are manufactured from steel girder and nets which are constructed by point welding. They are designed to indicate the necessary solidity and the required load bearing capacity of the cargo. The application of roof racks is universal and these products are in great demand in the field of game management. There are a lot of trailer hitches which the manufacturers tailor to customers´ demands. The stated technical solution is subject of published industrial utility model at the Industrial property office of the Slovak republic.

19 CFR 19.41 - Movement of containerized cargo to a container station.

Science.gov (United States)

2010-04-01

... 19 Customs Duties 1 2010-04-01 2010-04-01 false Movement of containerized cargo to a container station. 19.41 Section 19.41 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND... THEREIN Container Stations § 19.41 Movement of containerized cargo to a container station. Containerized...

Modeling of electronic power steering system for IKCO SAMAND vehicle and investigating on its performance via CARSIM software

Science.gov (United States)

Haghgoo, Esmail; Zamani, Mohammad; Sharbati, Ali

2017-02-01

The point of this article is introducing the usage of electronic power steering (ESP) system in IKCO SAMAND vehicle and investigating on it's benefit's. Also the operation of electronic steering system and it's performance in IKCO SAMAND vehicle have been described. The optimization of IC engine efficiency and it's fuel consumption have been simulated via ADVISOR software used in MATLAB software. Usually, mechanical steering systems and hydraulic steering systems are producing inside IRAN that the mechanical types have not accepted because of it's too many disadvantages. The hydraulic steering systems, that have been replaced with mechanical types, indeed have the same features with mechanical types but with a difference which they have a hydraulic booster to facilitate the rotation of steering wheel. Beside advantages in hydraulic systems, they are some disadvantages in this system that one of the most important of them is reducing the output power of engine. To restore this power dissipated, we use ESP systems. In this article output diagrams given by software, are showing that IKCO SAMAND vehicle which equipped with ESP system, exerts less torque and power on steering wheel. This improves the safety of driver and also performance of the vehicle at high speeds and reduces fuel consumption beside increasing the efficiency of IC engine.

Effects and limitations of a nucleobase-driven backmapping procedure for nucleic acids using steered molecular dynamics.

Science.gov (United States)

Poblete, Simón; Bottaro, Sandro; Bussi, Giovanni

2018-03-29

Coarse-grained models can be of great help to address the problem of structure prediction in nucleic acids. On one hand they can make the prediction more efficient, while on the other hand they can also help to identify the essential degrees of freedom and interactions for the description of a number of structures. With the aim to provide an all-atom representation in an explicit solvent to the predictions of our SPlit and conQueR (SPQR) coarse-grained model of RNA, we recently introduced a backmapping procedure which enforces the predicted structure into an atomistic one by means of steered molecular dynamics. These simulations minimize the ERMSD, a particular metric which deals exclusively with the relative arrangement of nucleobases, between the atomistic representation and the target structure. In this paper, we explore the effects of this approach on the resulting interaction networks and backbone conformations by applying it on a set of fragments using as a target their native structure. We find that the geometry of the target structures can be reliably recovered, with limitations in the regions with unpaired bases such as bulges. In addition, we observe that the folding pathway can also change depending on the parameters used in the definition of the ERMSD and the use of other metrics such as the RMSD. Copyright © 2017 Elsevier Inc. All rights reserved.

Networked Control System for the Guidance of a Four-Wheel Steering Agricultural Robotic Platform

Directory of Open Access Journals (Sweden)

Eduardo Paciência Godoy

2012-01-01

Full Text Available A current trend in the agricultural area is the development of mobile robots and autonomous vehicles for precision agriculture (PA. One of the major challenges in the design of these robots is the development of the electronic architecture for the control of the devices. In a joint project among research institutions and a private company in Brazil a multifunctional robotic platform for information acquisition in PA is being designed. This platform has as main characteristics four-wheel propulsion and independent steering, adjustable width, span of 1,80 m in height, diesel engine, hydraulic system, and a CAN-based networked control system (NCS. This paper presents a NCS solution for the platform guidance by the four-wheel hydraulic steering distributed control. The control strategy, centered on the robot manipulators control theory, is based on the difference between the desired and actual position and considering the angular speed of the wheels. The results demonstrate that the NCS was simple and efficient, providing suitable steering performance for the platform guidance. Even though the simplicity of the NCS solution developed, it also overcame some verified control challenges in the robot guidance system design such as the hydraulic system delay, nonlinearities in the steering actuators, and inertia in the steering system due the friction of different terrains.

The use of x-ray imaging technology in cargo inspection

International Nuclear Information System (INIS)

Palgan, Renato D.

2009-01-01

The aftermath of the September 11,2001 terrorist attack in the United States has brought a significant change in the conduct of trade and commerce among nations. The introduction on non-intrusive cargo inspection equipment, most x-ray and gamma ray scanners, among customs administrations worldwide has been widely employed and incorporated in their respective cargo examination procedures as part of their security initiative to deter terrorist threats. In compliance with our international commitment and agreement passed by the World Customs Organization which required the use of modern technology in cargo inspection, the Bureau of Customs had acquired 30 units of container x-ray scanners in 2006 from China that became operational in 2007 and the acquisition of which was guided by the following objectives: maximize collection of government revenues,facilitate trade and commerce, prevent smuggling, and safeguard the public and environment against the entry of hazardous and harmful substance. In this light, this paper discusses the brief background of the non-intrusive container inspection system project of the Bureau of Customs, an overview of the technical features of x-ray imaging technology acquired, the procedures in the application of x-ray inspection in containerized cargo, the radiation safety infrastructures and considerations, and a review of some major accomplishments after its two years of existence. (author)

The Expression of Adipogenic Genes in Adipose Tissues of Feedlot Steers Fed Supplementary Palm Oil or Soybean Oil.

Science.gov (United States)

Choi, Seong Ho; Park, Sung Kwon; Choi, Chang Weon; Li, Xiang Zi; Kim, Kyoung Hoon; Kim, Won Young; Jeong, Joon; Johnson, Bradley J; Zan, Linsen; Smith, Stephen B

2016-03-01

We hypothesized that supplementing finishing diets with palm oil would promote adipogenic gene expression and stearoyl-CoA desaturase (SCD) gene expression in subcutaneous (s.c.) and intramuscular (i.m.) adipose tissues of feedlot steers. Eighteen Angus and Angus crossbred steers were assigned to three groups of 6 steers and fed a basal diet (control), with 3% palm oil, or with 3% soybean oil, for 70 d, top-dressed daily. Tailhead s.c. adipose tissue was obtained by biopsy at 14 d before the initiation of dietary treatments and at 35 d of dietary treatments. At slaughter, after 70 d of dietary treatment, tailhead s.c. adipose tissue and i.m. adipose tissue were obtained from the longissimus thoracis muscle. Palm oil increased plasma palmitic acid and soybean oil increased plasma linoleic acid and α-linolenic acid relative to the initial sampling time. Expression of AMP-activated protein kinase alpha (AMPKα) and peroxisome proliferator-activated receptor gamma (PPARγ) increased between the initial and intermediate biopsies and declined thereafter (poil decreased (p = 0.01) PPARγ gene expression at the intermediate sample time. At the terminal sample time, PPARγ and SCD gene expression was less in i.m. adipose tissue than in s.c. adipose tissue (ppalm oil-fed steers than in control steers (p = 0.04) and CCAAT enhancer binding protein-beta (CEBPβ) gene expression was less in s.c. and i.m. adipose tissues of palm oil-fed steers than in soybean oil-fed steers (poil decreased SCD gene expression in s.c. adipose tissue (p = 0.05); SCD gene expression in palm oil-fed steers was intermediate between control and soybean oil-fed steers. Contrary to our original hypothesis, palm oil did not promote adipogenic gene expression in s.c. and i.m. adipose tissue.

46 CFR 31.40-10 - Cargo Ship Safety Equipment Certificate-T/ALL.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo Ship Safety Equipment Certificate-T/ALL. 31.40-10 Section 31.40-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-10 Cargo Ship...

46 CFR 31.40-5 - Cargo Ship Safety Construction Certificate-T/ALL.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo Ship Safety Construction Certificate-T/ALL. 31.40-5 Section 31.40-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-5 Cargo Ship...

46 CFR 31.40-15 - Cargo Ship Safety Radio Certificate-T/ALL.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Cargo Ship Safety Radio Certificate-T/ALL. 31.40-15 Section 31.40-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS INSPECTION AND CERTIFICATION Certificates Under International Convention for Safety of Life at Sea, 1974 § 31.40-15 Cargo Ship...

Alteration in gene expression in the jejunum mucosa of Angus steers with divergent ADG

Science.gov (United States)

The objective of this study was to determine the association of differentially expressed genes in the jejunum of steers with average DMI and high or low ADG. Feed intake and growth were measured in a cohort of 144 commercial Angus steers consuming a finishing ration containing (on a DM basis) 67.8% ...

Excitation Patterns of Standard and Steered Partial Tripolar Stimuli in Cochlear Implants

OpenAIRE

Wu, Ching-Chih; Luo, Xin

2015-01-01

Current steering in partial tripolar (pTP) mode has been shown to improve pitch perception and spectral resolution with cochlear implants (CIs). In this mode, a fraction (?) of the main electrode current is returned within the cochlea and steered between the basal and apical flanking electrodes (with a proportion of ? and 1????, respectively). Pitch generally decreases when ? increases from 0 to 1, although the salience of pitch change varies across CI users. This study aimed to identify the ...

Driver assistance system for lane departure avoidance by steering and differential braking

OpenAIRE

MINOIU-ENACHE, N; MAMMAR, S; GLASER, S; LUSETTI, B

2010-01-01

Lane departure avoidance systems assist actively the driver during inattention or drowsiness and increase driving safety. Most of the lane departure avoidance systems use for the lateral control of the vehicle in closed loop a DC motor similar to the electrical powered steering (EPS) assistance. Important difficulties and limits of this approach are the shared control with the driver on the steering wheel and the vehicle handling at limits. In this paper a combined lateral control using a DC ...

Steering Dynamics in the Dutch Education System

Science.gov (United States)

Waslander, Sietske; Hooge, Edith; Drewes, Tineke

2016-01-01

Based on detailed empirical analyses, we paint a layered picture of emerging steering dynamics. Inspired by Foucault, we put the focus on roles stakeholders define both for themselves and others, how they give sense to policy, how they work together in policy elaboration and implementation, and the subtle and sometimes deceitful function of soft…

Use of free steered vehicles. [United Kingdom

Energy Technology Data Exchange (ETDEWEB)

Ferris, W

1986-01-01

The UK National Coal Board's Western Area is currently producing 10.5 m/tons per annum from 16 collieries exploiting 26 different seams. This article looks at the use of free steered vehicles (FSV's) in Haig Colliery, Point of Ayr and Silverdale Colliery, covering general applications, installation of coal faces and face salvage by FSV's.

Comparison of vibration damping of standard and PDCPD housing of the electric power steering system

Science.gov (United States)

Płaczek, M.; Wróbel, A.; Baier, A.

2017-08-01

A comparison of two different types of electric power steering system housing is presented. The first considered type of the housing was a standard one that is made of an aluminium alloy. The second one is made of polydicyclopentadiene polymer (PDCPD) and was produced using the RIM technology. Considered elements were analysed in order to verify their properties of vibrations damping. This property is very important taking into account noise generated by elements of a car’s power steering system. During the carried out tests vibrations of analysed power steering housings were measured using Marco Fiber Composite (MFC) piezoelectric transducers. Results obtained for both considered power steering housings in case of the same parameters of vibrations excitations were measured and juxtaposed. Obtained results were analysed in order to verify if the housing made of PDCPD polymer has better properties of vibration damping than the standard one.

Progress on the ITER ECRH upper launcher steering mirror identification and control

International Nuclear Information System (INIS)

Collazos, Andres; Bertizzolo, Robert; Chavan, Rene; Dolizy, Frederic; Felici, Federico; Henderson, Mark A.; Landis, Jean-Daniel; Sanchez, Francisco

2009-01-01

The main objective of the ITER ECRH upper launcher (UL) is to control magnetohydrodynamic activity, in particular neoclassical tearing modes (NTMs), by driving several MW of EC current near the q = 1, 3/2, 2 flux surfaces, where NTMs are expected to occur. The steering of the EC power is done by the steering mechanism assembly (SMA) that comprises a reflecting mirror and a frictionless and backlash free pneumo-mechanical system actuated with pressurised helium gas. The control requirements for this component in terms of steering accuracy and speed are reviewed. With respect to these requirements, the performance of the first SMA prototype is assessed in a mock up of the UL pneumatic configuration. The expected design characteristics of the SMA have been verified and an overall satisfactory performance has been assessed. Furthermore, the main challenges for the future work, such as the pressure and angular position control, have been identified.

DETERMINATION OF STEERING WHEEL ANGLES DURING CAR ALIGNMENT BY IMAGE ANALYSIS METHODS

Directory of Open Access Journals (Sweden)

M. Mueller

2016-06-01

Full Text Available Optical systems for automatic visual inspections are of increasing importance in the field of automation in the industrial domain. A new application is the determination of steering wheel angles during wheel track setting of the final inspection of car manufacturing. The camera has to be positioned outside the car to avoid interruptions of the processes and therefore, oblique images of the steering wheel must be acquired. Three different approaches of computer vision are considered in this paper, i.e. a 2D shape-based matching (by means of a plane to plane rectification of the oblique images and detection of a shape model with a particular rotation, a 3D shape-based matching approach (by means of a series of different perspectives of the spatial shape of the steering wheel derived from a CAD design model and a point-to-point matching (by means of the extraction of significant elements (e.g. multifunctional buttons of a steering wheel and a pairwise connection of these points to straight lines. The HALCON system (HALCON, 2016 was used for all software developments and necessary adaptions. As reference a mechanical balance with an accuracy of 0.1° was used. The quality assessment was based on two different approaches, a laboratory test and a test during production process. In the laboratory a standard deviation of ±0.035° (2D shape-based matching, ±0.12° (3D approach and ±0.029° (point-to-point matching could be obtained. The field test of 291 measurements (27 cars with varying poses and angles of the steering wheel results in a detection rate of 100% and ±0.48° (2D matching and ±0.24° (point-to-point matching. Both methods also fulfil the request of real time processing (three measurements per second.

Optimization Under Uncertainty for Wake Steering Strategies

Energy Technology Data Exchange (ETDEWEB)

Quick, Julian [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Annoni, Jennifer [National Renewable Energy Laboratory (NREL), Golden, CO (United States); King, Ryan N [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Dykes, Katherine L [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Fleming, Paul A [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Ning, Andrew [Brigham Young University

2017-08-03

Offsetting turbines' yaw orientations from incoming wind is a powerful tool that may be leveraged to reduce undesirable wake effects on downstream turbines. First, we examine a simple two-turbine case to gain intuition as to how inflow direction uncertainty affects the optimal solution. The turbines are modeled with unidirectional inflow such that one turbine directly wakes the other, using ten rotor diameter spacing. We perform optimization under uncertainty (OUU) via a parameter sweep of the front turbine. The OUU solution generally prefers less steering. We then do this optimization for a 60-turbine wind farm with unidirectional inflow, varying the degree of inflow uncertainty and approaching this OUU problem by nesting a polynomial chaos expansion uncertainty quantification routine within an outer optimization. We examined how different levels of uncertainty in the inflow direction effect the ratio of the expected values of deterministic and OUU solutions for steering strategies in the large wind farm, assuming the directional uncertainty used to reach said OUU solution (this ratio is defined as the value of the stochastic solution or VSS).

Entanglement, Einstein-Podolsky-Rosen correlations, Bell nonlocality, and steering

International Nuclear Information System (INIS)

Jones, S. J.; Wiseman, H. M.; Doherty, A. C.

2007-01-01

In a recent work [Phys. Rev. Lett. 98, 140402 (2007)] we defined 'steering', a type of quantum nonlocality that is logically distinct from both nonseparability and Bell nonlocality. In the bipartite setting, it hinges on the question of whether Alice can affect Bob's state at a distance through her choice of measurement. More precisely and operationally, it hinges on the question of whether Alice, with classical communication, can convince Bob that they share an entangled state under the circumstances that Bob trusts nothing that Alice says. We argue that if she can, then this demonstrates the nonlocal effect first identified in the famous Einstein-Podolsky-Rosen paper [Phys. Rev. 47, 777 (1935)] as a universal effect for pure entangled states. This ability of Alice to remotely prepare Bob's state was subsequently called steering by Schroedinger, whose terminology we adopt. The phenomenon of steering has been largely overlooked, and prior to our work had not even been given a rigorous definition that is applicable to mixed states as well as pure states. Armed with our rigorous definition, we proved that steerable states are a strict subset of the entangled states, and a strict superset of the states that can exhibit Bell nonlocality. In this work we expand on these results and provide further examples of steerable states. We also elaborate on the connection with the original EPR paradox

Steering Control in a Low-Cost Driving Simulator: A Case for the Role of Virtual Vehicle Cab.

Science.gov (United States)

Mecheri, Sami; Lobjois, Régis

2018-04-01

The aim of this study was to investigate steering control in a low-cost driving simulator with and without a virtual vehicle cab. In low-cost simulators, the lack of a vehicle cab denies driver access to vehicle width, which could affect steering control, insofar as locomotor adjustments are known to be based on action-scaled visual judgments of the environment. Two experiments were conducted in which steering control with and without a virtual vehicle cab was investigated in a within-subject design, using cornering and straight-lane-keeping tasks. Driving around curves without vehicle cab information made drivers deviate more from the lane center toward the inner edge in right (virtual cab = 4 ± 19 cm; no cab = 42 ± 28 cm; at the apex of the curve, p vehicle width. This produces considerable differences in the steering trajectory. Providing a virtual vehicle cab must be encouraged for more effectively capturing drivers' steering control in low-cost simulators.

Investigation on a Power Coupling Steering System for Dual-Motor Drive Tracked Vehicles Based on Speed Control

Directory of Open Access Journals (Sweden)

Li Zhai

2017-08-01

Full Text Available Double-motor drive tracked vehicles (2MDTV are widely used in the tracked vehicle industry due to the development of electric vehicle drive systems. The aim of this paper is to solve the problem of insufficient propulsion motor torque in low-speed, small-radius steering and insufficient power in high-speed large-radius steering. In order to do this a new type of steering system with a coupling device is designed and a closed-loop control strategy based on speed is adopted to improve the lateral stability of the vehicle. The work done entails modeling and simulating the 2MDTV and the proposed control strategy in RecurDyn and Matlab/Simulink. The simulation results show that the 2MDTV with the coupling device outputs more torque and power in both steering cases compared to the 2MDTV without the coupling device, and the steering stability of the vehicle is improved by using the strategy based on speed.

GMTIFS: the adaptive optics beam steering mirror for the GMT integral-field spectrograph

Science.gov (United States)

Davies, J.; Bloxham, G.; Boz, R.; Bundy, D.; Espeland, B.; Fordham, B.; Hart, J.; Herrald, N.; Nielsen, J.; Sharp, R.; Vaccarella, A.; Vest, C.; Young, P. J.

2016-07-01

To achieve the high adaptive optics sky coverage necessary to allow the GMT Integral-Field Spectrograph (GMTIFS) to access key scientific targets, the on-instrument adaptive-optics wavefront-sensing (OIWFS) system must patrol the full 180 arcsecond diameter guide field passed to the instrument. The OIWFS uses a diffraction limited guide star as the fundamental pointing reference for the instrument. During an observation the offset between the science target and the guide star will change due to sources such as flexure, differential refraction and non-sidereal tracking rates. GMTIFS uses a beam steering mirror to set the initial offset between science target and guide star and also to correct for changes in offset. In order to reduce image motion from beam steering errors to those comparable to the AO system in the most stringent case, the beam steering mirror is set a requirement of less than 1 milliarcsecond RMS. This corresponds to a dynamic range for both actuators and sensors of better than 1/180,000. The GMTIFS beam steering mirror uses piezo-walk actuators and a combination of eddy current sensors and interferometric sensors to achieve this dynamic range and control. While the sensors are rated for cryogenic operation, the actuators are not. We report on the results of prototype testing of single actuators, with the sensors, on the bench and in a cryogenic environment. Specific failures of the system are explained and suspected reasons for them. A modified test jig is used to investigate the option of heating the actuator and we report the improved results. In addition to individual component testing, we built and tested a complete beam steering mirror assembly. Testing was conducted with a point source microscope, however controlling environmental conditions to less than 1 micron was challenging. The assembly testing investigated acquisition accuracy and if there was any un-sensed hysteresis in the system. Finally we present the revised beam steering mirror

Alfalfa leaf meal in finishing steer diets. Quarterly report, July 1, 1997--September 30, 1997

Energy Technology Data Exchange (ETDEWEB)

Zehnder, C.M.; DiCostanzo, A.; Smith, L.B.; Brown, D.B.; Hall, J.M.

1997-10-30

Ninety-six medium frame, Angus and Angus cross steer calves (average initial weight 540 lb.) were allotted to a heavy or light weight block and then randomly assigned to one of four dietary treatments for a 167 or 189-day finishing phase, respectively. Treatments were control (supplemental soybean meal), alfalfa leaf meal (ALM) providing 33%, 66%, 100% of supplemental protein. Finishing diets were formulated to contain .61 Mcal NE{sub g}/lb dry matter, 12.5% crude protein, .6 % Ca and .3 % P. There were no significant (P >.05) effects of dietary treatments on daily gain or dry matter required /lb of gain. Steers fed 100 % ALM consumed more (P <.05) dry matter than steers fed either of the other three treatments. Dry matter consumption increased linearly (P >.05) with increasing ALM. There was no significant (P >.05) dietary treatment effect on marbling, KPH %, yield grade, quality grade, or liver abscesses. There was an apparent trend in reduced liver abscess incidence in steers fed 100 % ALM. Steers fed 66 % ALM had significantly (P <.05) greater backfat measurements, backfat also had a cubic effect (P <.05). Hot carcass weight had a quadratic relation (P <.05) with level of ALM. Substituting alfalfa leaf meal for soybean meal in diets of finishing steers increased DM intake, but this increase was accompanied by an increase in gain which resulted in similar feed efficiency. There may be an advantage in blending ALM and soybean meal as feed efficiency was improved when cattle were fed the blend. Also, feeding ALM may result in lower incidence of liver abscess.

Analysis of forecasting methods of cargo flows in International transportation by land transport

OpenAIRE

Ponomareva, N.

2005-01-01

Advantages and disadvantages of the existing forecasting methods of cargo flows are presented. The improvement of cargo flows forecasting method in international transportation by land transport is considered on the basis of a interregional balance model to get more correct and fuller forecast.

Dangers of releasing CO₂ to fight fires in the cargo hold of seagoing bulk carriers

DEFF Research Database (Denmark)

Hedlund, Frank Huess; Jarleivson Hilduberg, Øssur

the application of CO₂ to deep-seated fires involving solids subject to smoldering, but without identifying or alerting the reader to the potential presence of explosive pyrolysis gases. NFPA 12 appears to presume that electrostatic discharges will dissipate safety if metal nozzles are used and the entire system......On seagoing general cargo vessels, the cargo is stored in bulk in the holds. Fire protection for cargo holds comprises detection and firefighting capability. Detection normally incorporates a smoke sampling system that continuously draws air from each cargo hold and passes it to a smoke detector...... cabinet. The fire can be fought by flooding the cargo hold with inert carbon dioxide. The carbon dioxide is stored in its liquid form at pressures in excess of 50 bar and kept in multiple vertical steel cylinders arranged in a battery. For firefighting to be effective, SOLAS regulations require...

Effects of steering demand on lane keeping behaviour, self-reports, and physiology : A simulator study

NARCIS (Netherlands)

Dijksterhuis, Chris; Brookhuis, Karel A.; De Waard, Dick

In this study a driving simulator was used to determine changes in mental effort in response to manipulations of steering demand. Changes in mental effort were assessed by using subjective effort ratings, physiology, and the standard deviation of the lateral position. Steering demand was increased

A Time Modulated Printed Dipole Antenna Array for Beam Steering Application

Directory of Open Access Journals (Sweden)

Ruchi Gahley

2017-01-01

Full Text Available This paper presents time modulated beam steered antenna array without phase shifters. The beam steering is analyzed considering a two-element time modulated antenna array (TMAA of printed dipoles with microstrip via-hole balun. The proposed array resonates at the Industrial, Scientific, and Medical (ISM radio bands, 2.45 GHz and 5.8 GHz, and offers wide bandwidth inherited due to modified structure of ground plane. Array elements are excited by complex exponential excitation signal through broadband power divider and radio frequency (RF switches to achieve amplitude and phase variation without phase shifters. Differential Evolution algorithm is used to modify the time sequences of the RF switches connected to the antennas to generate radiation pattern with optimum dynamic efficiency by suppressing sideband radiations. Also switch-on time instant of RF switch connected to the subsequent element is modulated to steer the beam towards different directions. The proposed prototype is fabricated followed by parametric optimization. The fabrication results agree significantly well with simulated results.

Spatial Steering of Cyclotron-Resonance Maser Array Antenna by Magnetic Fields

International Nuclear Information System (INIS)

Kesar, A.; Jerby, E.

2001-01-01

The novel concept of radiation lobe generation and steering by cyclotron-resonance maser (CRM) array is presented. In this scheme the gain and phase of each CRM-element in the array are tuned by magnetic fields which control the cyclotron synchronism condition and the pitch-ratio of each CRM-element. These operating parameters are controlled by the magnetic fields of the solenoid and the kicker, respectively. A numerical example of a CRM-array operating in a gyro-TWT mode is presented. The radiation pattern of a 10-element CRM phased array (15 kV, 1A each) is calculated. The radiation lobe steering by the magnetic field controls is demonstrated in this analysis. A 40 lobe steering range is shown for the 10-element CRM-array at 7.3 GHz. An experimental device is built in our laboratory to demonstrate the active CRM-array antenna concept. Preliminary experimental results of gain and phase-delay of a single CRM-element, as function of electron-beam parameters are presented. These results are compared to the numerical model

Automated vehicle guidance using discrete reference markers. [road surface steering techniques

Science.gov (United States)

Johnston, A. R.; Assefi, T.; Lai, J. Y.

1979-01-01

Techniques for providing steering control for an automated vehicle using discrete reference markers fixed to the road surface are investigated analytically. Either optical or magnetic approaches can be used for the sensor, which generates a measurement of the lateral offset of the vehicle path at each marker to form the basic data for steering control. Possible mechanizations of sensor and controller are outlined. Techniques for handling certain anomalous conditions, such as a missing marker, or loss of acquisition, and special maneuvers, such as u-turns and switching, are briefly discussed. A general analysis of the vehicle dynamics and the discrete control system is presented using the state variable formulation. Noise in both the sensor measurement and in the steering servo are accounted for. An optimal controller is simulated on a general purpose computer, and the resulting plots of vehicle path are presented. Parameters representing a small multipassenger tram were selected, and the simulation runs show response to an erroneous sensor measurement and acquisition following large initial path errors.

Nonlinear roll damping of a barge with and without liquid cargo in spherical tanks

Directory of Open Access Journals (Sweden)

Wenhua Zhao

2016-01-01

Full Text Available Damping plays a significant role on the maximum amplitude of a vessel's roll motion, in particular near the resonant frequency. It is a common practice to predict roll damping using a linear radiation–diffraction code and add that to a linearized viscous damping component, which can be obtained through empirical, semi-empirical equations or free decay tests in calm water. However, it is evident that the viscous roll damping is nonlinear with roll velocity and amplitude. Nonlinear liquid cargo motions inside cargo tanks also contribute to roll damping, which when ignored impedes the accurate prediction of maximum roll motions. In this study, a series of free decay model tests is conducted on a barge-like vessel with two spherical tanks, which allows a better understanding of the nonlinear roll damping components considering the effects of the liquid cargo motion. To examine the effects of the cargo motion on the damping levels, a nonlinear model is adopted to calculate the damping coefficients. The liquid cargo motion is observed to affect both the linear and the quadratic components of the roll damping. The flow memory effect on the roll damping is also studied. The nonlinear damping coefficients of the vessel with liquid cargo motions in spherical tanks are obtained, which are expected to contribute in configurations involving spherical tanks.

Design of a remote steering antenna for ECRH heating in the stellarator Wendelstein 7-X

Energy Technology Data Exchange (ETDEWEB)

Plaum, B., E-mail: plaum@igvp.uni-stuttgart.de [Institut für Grenzflächenverfahrenstechnik und Plasmatechnologie (IGVP), Univ. Stuttgart, Pfaffenwaldring 31, D-70569 Stuttgart (Germany); Lechte, C.; Kasparek, W.; Gaiser, S.; Zeitler, A. [Institut für Grenzflächenverfahrenstechnik und Plasmatechnologie (IGVP), Univ. Stuttgart, Pfaffenwaldring 31, D-70569 Stuttgart (Germany); Erckmann, V. [Max-Planck-Institut für Plasmaphysik, EURATOM-IPP, D-17491 Greifswald (Germany); Weißgerber, M. [Max-Planck-Institut für Plasmaphysik, EURATOM-IPP, D-85748 Garching (Germany); Bechtold, A. [NTG Neue Technologie GmbH & Co KG, D-63571 Gelnhausen (Germany); Busch, M.; Szcepaniak, B. [Galvano-T electroplating-electroforming GmbH, D-51570 Windeck-Rosbach (Germany)

2015-10-15

Highlights: • We report about the design activities for the remote steering antennas for the stellarator W7-X. • The integration into the W7-X system and the manufacturing procedure are described. • Simulations and loss measurements for the waveguide walls were done and are in good agreement. • A method for extending the steering range is presented. • A mechanical deformation analysis showed that the deformation is not critical for the beam quality. - Abstract: For the ECRH heating system of the stellarator Wendelstein 7-X, two remote steering antennas are developed and manufactured. The principle of remote steering antennas is based on the imaging characteristics of corrugated rectangular waveguides, which is well understood and can accurately be simulated. Several details, however, require deeper investigation. The antenna needs a miter-bend and a 24 mm gap. The positions of these elements need to be chosen carefully to reduce losses and stray radiation. The antennas are manufactured from copper by electroforming. This allows to integrate all components, including the corrugated inner walls and the cooling channels, in one vacuum-tight piece. This paper reviews the design process of the remote steering antennas for W7-X as well as technological issues and experimental results from test pieces.

Quantum steering of multimode Gaussian states by Gaussian measurements: monogamy relations and the Peres conjecture

International Nuclear Information System (INIS)

Ji, Se-Wan; Nha, Hyunchul; Kim, M S

2015-01-01

It is a topic of fundamental and practical importance how a quantum correlated state can be reliably distributed through a noisy channel for quantum information processing. The concept of quantum steering recently defined in a rigorous manner is relevant to study it under certain circumstances and here we address quantum steerability of Gaussian states to this aim. In particular, we attempt to reformulate the criterion for Gaussian steering in terms of local and global purities and show that it is sufficient and necessary for the case of steering a 1-mode system by an N-mode system. It subsequently enables us to reinforce a strong monogamy relation under which only one party can steer a local system of 1-mode. Moreover, we show that only a negative partial-transpose state can manifest quantum steerability by Gaussian measurements in relation to the Peres conjecture. We also discuss our formulation for the case of distributing a two-mode squeezed state via one-way quantum channels making dissipation and amplification effects, respectively. Finally, we extend our approach to include non-Gaussian measurements, more precisely, all orders of higher-order squeezing measurements, and find that this broad set of non-Gaussian measurements is not useful to demonstrate steering for Gaussian states beyond Gaussian measurements. (paper)

Steering with big words: articulating ideographs in nanotechnology

NARCIS (Netherlands)

Bos, Colette; Walhout, Albert; Peine, Alex; van Lente, Harro

2014-01-01

Nowadays, science should address societal challenges, such as ‘sustainability’, or ‘responsible research and innovation’. This emerging form of steering toward broad and generic goals involves the use of ‘big words’: encompassing concepts that are uncontested themselves, but that allow for multiple

Development of steering system in network environment

International Nuclear Information System (INIS)

Kanagawa, Fumihiro; Noguchi, So; Yamashita, Hideo

2002-01-01

We have been developing the steering system, which can successively observe the-data obtained during the numerical computation and change the parameters in the analysis. Moreover, this system is also extended to link the network. By using this system, a user can easily detect errors immediately and achieve the rapid and accurate analysis with lower computation cost. (Author)

The GRIP1/14-3-3 pathway coordinates cargo trafficking and dendrite development

NARCIS (Netherlands)

Geiger, J.C.; Lipka, Joanna; Hoyer, S.; Schlager, M.A.; Wulf, Phebe; Weinges, S.; Demmers, J.; Hoogenraad, Casper

2014-01-01

Regulation of cargo transport via adaptor molecules is essential for neuronal development. However, the role of PDZ scaffolding proteins as adaptors in neuronal cargo trafficking is still poorly understood. Here, we show by genetic deletion in mice that the multi-PDZ domain scaffolding protein