WorldWideScience

Sample records for stammes b1 im

  1. SAFIRA. Sub-project B 1.3: Development of coupled in-situ reactors and optimisation of the geochemical processes in the discharge of different in situ reactor sytems. Final report; SAFIRA. Teilprojekt B 1.3: Entwicklung von gekoppelten in situ-Reaktoren und Optimierung der geochemischen Prozesse im Abstrom von verschiedenen in situ-Reaktor-Systemen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Dahmke, A.; Schaefer, D.; Koeber, R.; Plagentz, V.

    2002-12-01

    The Bitterfeld ground water is contaminated with many different pollutants over a large area. Long-term measures like reactive barriers for purification are required. However, groundwater contaminated with multiple contaminants cannot be purified by a single reactive material; for this reason, the effectivity of combinations of different reactive materials was investigated. Of the combinations investigated, reducing iron and activated carbon connected in series was the most effective: The iron will remove the reducible chlorinated hydrocarbons, while the rest of the contaminants are adsorbed to the activated carbon. Iron and ORC was another interesting option, but the combination of iron and activated carbon was found to be the most favourable option. Until a better method is available, it is recommended to connect iron and activated carbon in parallel for removing contaminant mixtures. Directly behind reactive iron barriers (also when combined with activated carbon), the limiting values of the Freshwater Ordinance for Fe(II) and pH are exceeded. Directly behind ORC reactors, the limiting values for Mg and pH are exceeded. Investigations in the outflow of these reactive materials showed that the high pH values are buffered by contact with the aquifer material to values typical of aquifers, which usually are below the limiting values of the Freshwater Ordinance. However, as the buffer capacity of the soil is exhausted, a zone with a higher pH starts to grow in the aquifer. The growth of this zone depends on the pH and on the aquifer material. Especially in soils as found at Bitterfeld, with a high concentration of organic matter, we find long-term desorption of pollutants from the aquifer materials which will burden the purified water leaving the water treatment system and prohibit its utilization. [German] Der Grundwasserleiter im Raum Bitterfeld ist grossraeumig mit vielen verschiedenen Substanzen kontaminiert. Aufgrund der grossraeumigen Erstreckung kommen nur

  2. Vitamin B1

    Science.gov (United States)

    ... Farm Game - Why do we need vitamin B1? - What food contains vitamin B1? - What is the disease beriberi? This fast-paced game relies on a keen knowledge of food containing vitamin B1 and good keyboard skills to ...

  3. IMS Interworking

    OpenAIRE

    Kalaglarski, Boris Iv.; Di Geronimo, Emilio

    2007-01-01

    The goal of this project was to analyze the IP Multimedia Subsystem (IMS) with respect to the interworking functionality between two or more IMS domains belonging to different operators. The thesis presents an overview of IMS, its purpose, the circumstances and the environment in which it has evolved, and a look into some of the challenges that lie ahead. Through careful examination of the history of the mobile communications and of IMS itself, the thesis attempts to give the reader a full an...

  4. Cyp8b1

    Science.gov (United States)

    Bertaggia, Enrico; Jensen, Kristian K; Castro-Perez, Jose; Xu, Yimeng; Di Paolo, Gilbert; Chan, Robin B; Wang, Liangsu; Haeusler, Rebecca A

    2017-08-01

    Bile acids (BAs) are cholesterol derivatives that regulate lipid metabolism, through their dual abilities to promote lipid absorption and activate BA receptors. However, different BA species have varying abilities to perform these functions. Eliminating 12α-hydroxy BAs in mice via Cyp8b1 knockout causes low body weight and improved glucose tolerance. The goal of this study was to determine mechanisms of low body weight in Cyp8b1 -/- mice. We challenged Cyp8b1 -/- mice with a Western-type diet and assessed body weight and composition. We measured energy expenditure, fecal calories, and lipid absorption and performed lipidomic studies on feces and intestine. We investigated the requirement for dietary fat in the phenotype using a fat-free diet. Cyp8b1 -/- mice were resistant to Western diet-induced body weight gain, hepatic steatosis, and insulin resistance. These changes were associated with increased fecal calories, due to malabsorption of hydrolyzed dietary triglycerides. This was reversed by treating the mice with taurocholic acid, the major 12α-hydroxylated BA species. The improvements in body weight and steatosis were normalized by feeding mice a fat-free diet. The effects of BA composition on intestinal lipid handling are important for whole body energy homeostasis. Thus modulating BA composition is a potential tool for obesity or diabetes therapy. Copyright © 2017 the American Physiological Society.

  5. CYP7B1

    DEFF Research Database (Denmark)

    Roos, P; Svenstrup, K; Danielsen, E R

    2014-01-01

    UNLABELLED: The SPG5A subtype of Hereditary Spastic Paraplegia (HSP) is a rare autosomal recessive neurodegenerative disorder caused by mutations in the CYP7B1 gene, which encodes a steroid cytochrome P450 7α-hydroxylase. This enzyme provides the primary metabolic route for neurosteroids......, electrophysiology, brain MRI and MR spectroscopy. RESULTS: One patient had saccadic pursuit eye movements in addition to a pure HSP phenotype. Motor evoked potential (MEP) examinations revealed prolonged central conduction time. MRI of the brain showed white matter hyperintensities (WMH) in one patient. MRS showed...

  6. aflatoxina b1

    Directory of Open Access Journals (Sweden)

    A. Valdivia

    2004-01-01

    Full Text Available Con el objetivo de probar que la suplementación dietética de ácido elágico (AE o N-Acetilcisteína (NAC en pollos de engorda, atenúa los efectos de una intoxicación aguda por la aflatoxina B1 (AFB1, se intoxicaron con AFB1 pura, tres grupos de diez pollos cada uno (3.0 mb/kg pc, IP. Otros tres grupos recibieron solamente el vehículo (aceite de maíz 2.0 ml/kg pc, IP. Cuatro días antes se administró un alimento testigo, o bien, la misma dieta adicionada con AE (2.5 g/kg o NAC (200 mg/kg pc/6 h. A las 24 horas de la administración de AFB1, se cuantificaron las concentraciones hepáticas de glutatión (GSH, de actividad enzimática específica de la transferasa de glutatión (GST, alanina aminotransferasa, aspartato aminotransferasa y de proteínas hepáticas totales. Los resultados mostraron que NAC atenúa el impacto negativo de la AFB1 sobre el crecimiento corporal y al igual que AE, incrementa la GST y revierte parcialmente los efectos de AFB1 sobre GSH, lo cual sugiere que ambas sustancias pudieran conferir un efecto protector de las aves

  7. B1 Aerogels

    DEFF Research Database (Denmark)

    Duer, Karsten; Svendsen, Sv Aa Højgaard

    1996-01-01

    , engineering and architectural basis which will support the appropriate use of aerogels in windows, solar collectors and passive solar applications, with the aim of saving or producing thermal energy for use in buildings".This objective is in very good agreement with the general scope of task 18 but where Task...... of aerogel as a material for window applications3. Construction of an aerogel DGU and measurement of key performance parameters. The goal for the aerogel DGU was to reach a Total Solar Energy Transmittance above 0.75 and a U-value below 0.5 W/m²K. These are values that can not be simultaneously reached......The report summarizes the work that has been carried out within the project "B1 AEROGELS" as a part of the IEA SH&CP Task 18 "Advanced Glazing and Associated Materials For Solar And Building Applications".By providing at the same time thermal insulation and transparency the silica aerogel is a very...

  8. Inhibiting Aspergillus flavus growth and degrading aflatoxin B1 by ...

    African Journals Online (AJOL)

    ajl2

    2012-08-14

    Aug 14, 2012 ... al., 2009). They have been detected in many food and oilseed commodities from many parts of the world and are presently considered as one of the most dangerous contaminants of food and feed. ..... Teniola OD, Addo PA, Brost IM, Farber P, Jany KD, Alberts JF (2005). Degradation of aflatoxin B1 by ...

  9. Muriithi, IM

    African Journals Online (AJOL)

    Muriithi, IM. Vol 8, No 2 (2014) - Articles Correlation of magnetic resonance imaging findings with arthroscopy in the evaluation of rotator cuff pathology. Abstract PDF · Vol 8, No 2 (2014) - Articles Patterns of knee, hip and hand osteoarthritis in Kenyatta National Hospital Abstract PDF. ISSN: 1994-1072. AJOL African ...

  10. Quick scan IMS vendors

    NARCIS (Netherlands)

    Norp, A.H.J.; Pals, H.; Walraven, F.A.

    2007-01-01

    IMS is gaining momentum in the market. First operators are deploying IMS, while other operators are considering buying IMS. Operators have two approaches for introducing IMS and building an IMS eco-system: • Buying IMS from a large vendor or system integrator. Vendors like Alcatel-Lucent, Ericsson

  11. Inductive Monitoring System (IMS)

    Data.gov (United States)

    National Aeronautics and Space Administration — IMS: Inductive Monitoring System The Inductive Monitoring System (IMS) is a tool that uses a data mining technique called clustering to extract models of normal...

  12. 7 CFR 15b.1 - Purpose.

    Science.gov (United States)

    2010-01-01

    ... FEDERAL FINANCIAL ASSISTANCE General Provisions § 15b.1 Purpose. The purpose of this part is to implement... 7 Agriculture 1 2010-01-01 2010-01-01 false Purpose. 15b.1 Section 15b.1 Agriculture Office of the... receiving Federal financial assistance. ...

  13. 15 CFR 8b.1 - Purpose.

    Science.gov (United States)

    2010-01-01

    ... of handicap in any program or activity receiving Federal financial assistance. The purpose of this... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Purpose. 8b.1 Section 8b.1 Commerce... HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE General Provisions § 8b.1...

  14. 34 CFR 5b.1 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... maintained by the Department, including but not limited to the individual's education, financial transactions... 34 Education 1 2010-07-01 2010-07-01 false Definitions. 5b.1 Section 5b.1 Education Office of the Secretary, Department of Education PRIVACY ACT REGULATIONS § 5b.1 Definitions. As used in this part: (a...

  15. Elementarteilchenphysik im Anfangsunterricht

    CERN Document Server

    AUTHOR|(CDS)2084439; Schmeling, Sascha; Hopf, Martin Richard

    2017-01-01

    In einem umfangreichen physikdidaktisches Forschungs- und Entwicklungsprojekt wurde untersucht, wie Teilchenphysik an junge Schülerinnen und Schüler vermittelt werden kann. Im Beitrag werden die verwendeten Materialien sowie einzelne Forschungsergebnisse vorgestellt.

  16. Jugendschutz im Internet

    OpenAIRE

    Wassmer, C

    2015-01-01

    Jugendschutz im Internet ist komplex und bedarf entsprechend wohlüberlegter Regeln und Regulierungsmechanismen. Wie die Debatte in deutschen und Schweizer Medien darüber geführt wird, zeigt eine Studie von Christian Wassmer.

  17. IMS - MS Data Extractor

    Energy Technology Data Exchange (ETDEWEB)

    2015-10-20

    An automated drift time extraction and computed associated collision cross section software tool for small molecule analysis with ion mobility spectrometry-mass spectrometry (IMS-MS). The software automatically extracts drift times and computes associated collision cross sections for small molecules analyzed using ion mobility spectrometry-mass spectrometry (IMS-MS) based on a target list of expected ions provided by the user.

  18. Aflatoxin B1 and Tobacco Products

    Directory of Open Access Journals (Sweden)

    Massey ED

    2014-12-01

    Full Text Available The potential for aflatoxin B1 contamination of tobaccos and its subsequent transfer to smoke has been raised. This paper examines published work relevant to this issue and concludes that aflatoxin B1 is not a contamination issue on tobaccos and, even if present, would decompose in the burning cigarette and would not transfer to smoke.

  19. 18 CFR 1b.1 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... Section 1b.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.1 Definitions. For purposes of this part— (a... pipelines, electric utilities and hydroelectric projects. [43 FR 27174, June 23, 1978, as amended by Order...

  20. Observation of B Meson decays to b1pi and b1K.

    Science.gov (United States)

    Aubert, B; Bona, M; Boutigny, D; Karyotakis, Y; Lees, J P; Poireau, V; Prudent, X; Tisserand, V; Zghiche, A; Tico, J Garra; Grauges, E; Lopez, L; Palano, A; Pappagallo, M; Eigen, G; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Button-Shafer, J; Cahn, R N; Groysman, Y; Jacobsen, R G; Kadyk, J A; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Pegna, D Lopes; Lynch, G; Mir, L M; Orimoto, T J; Osipenkov, I L; Ronan, M T; Tackmann, K; Tanabe, T; Wenzel, W A; Del Amo Sanchez, P; Hawkes, C M; Watson, A T; Held, T; Koch, H; Pelizaeus, M; Schroeder, T; Steinke, M; Walker, D; Asgeirsson, D J; Cuhadar-Donszelmann, T; Fulsom, B G; Hearty, C; Mattison, T S; McKenna, J A; Barrett, M; Khan, A; Saleem, M; Teodorescu, L; Blinov, V E; Bukin, A D; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M; Martin, E C; Stoker, D P; Abachi, S; Buchanan, C; Foulkes, S D; Gary, J W; Liu, F; Long, O; Shen, B C; Zhang, L; Paar, H P; Rahatlou, S; Sharma, V; Berryhill, J W; Campagnari, C; Cunha, A; Dahmes, B; Hong, T M; Kovalskyi, D; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Schalk, T; Schumm, B A; Seiden, A; Wilson, M G; Winstrom, L O; Chen, E; Cheng, C H; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Andreassen, R; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Blanc, F; Bloom, P C; Chen, S; Ford, W T; Hirschauer, J F; Kreisel, A; Nagel, M; Nauenberg, U; Olivas, A; Smith, J G; Ulmer, K A; Wagner, S R; Zhang, J; Gabareen, A M; Soffer, A; Toki, W H; Wilson, R J; Winklmeier, F; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Klose, V; Kobel, M J; Lacker, H M; Mader, W F; Nogowski, R; Schubert, J; Schubert, K R; Schwierz, R; Sundermann, J E; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Lombardo, V; Thiebaux, Ch; Verderi, M; Clark, P J; Gradl, W; Muheim, F; Playfer, S; Robertson, A I; Watson, J E; Xie, Y; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppi, E; Negrini, M; Petrella, A; Piemontese, L; Prencipe, E; Santoro, V; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; de Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Chaisanguanthum, K S; Morii, M; Wu, J; Dubitzky, R S; Marks, J; Schenk, S; Uwer, U; Bard, D J; Dauncey, P D; Flack, R L; Nash, J A; Vazquez, W Panduro; Tibbetts, M; Behera, P K; Chai, X; Charles, M J; Mallik, U; Ziegler, V; Cochran, J; Crawley, H B; Dong, L; Eyges, V; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gao, Y Y; Gritsan, A V; Guo, Z J; Lae, C K; Denig, A G; Fritsch, M; Schott, G; Arnaud, N; Béquilleux, J; D'Orazio, A; Davier, M; Grosdidier, G; Höcker, A; Lepeltier, V; Le Diberder, F; Lutz, A M; Pruvot, S; Rodier, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wang, W F; Wormser, G; Lange, D J; Wright, D M; Bingham, I; Burke, J P; Chavez, C A; Forster, I J; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Schofield, K C; Touramanis, C; Bevan, A J; George, K A; Di Lodovico, F; Menges, W; Sacco, R; Cowan, G; Flaecher, H U; Hopkins, D A; Paramesvaran, S; Salvatore, F; Wren, A C; Brown, D N; Davis, C L; Allison, J; Barlow, N R; Barlow, R J; Chia, Y M; Edgar, C L; Lafferty, G D; West, T J; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Blaylock, G; Dallapiccola, C; Hertzbach, S S; Li, X; Moore, T B; Salvati, E; Saremi, S; Cowan, R; Dujmic, D; Fisher, P H; Koeneke, K; Sciolla, G; Sekula, S J; Spitznagel, M; Taylor, F; Yamamoto, R K; Zhao, M; Zheng, Y; McLachlin, S E; Patel, P M; Robertson, S H; Lazzaro, A; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Brunet, S; Côté, D; Simard, M; Taras, P; Viaud, F B; Nicholson, H; De Nardo, G; Fabozzi, F; Lista, L; Monorchio, D; Sciacca, C; Baak, M A; Raven, G; Snoek, H L; Jessop, C P; Knoepfel, K J; Losecco, J M; Benelli, G; Corwin, L A; Honscheid, K; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Wong, Q K; Blount, N L; Brau, J; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Gagliardi, N; Gaz, A; Margoni, M; Morandin, M; Pompili, A; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Ben-Haim, E; Briand, H; Calderini, G; Chauveau, J; David, P; Del Buono, L; de la Vaissière, Ch; Hamon, O; Leruste, Ph; Malclès, J; Ocariz, J; Perez, A; Prendki, J; Gladney, L; Biasini, M; Covarelli, R; Manoni, E; Angelini, C; Batignani, G; Bettarini, S; Carpinelli, M; Cenci, R; Cervelli, A; Forti, F

    2007-12-14

    We present the results of searches for decays of B mesons to final states with a b1 meson and a charged pion or kaon. The data, collected with the BABAR detector at the Stanford Linear Accelerator Center, represent 382x10(6) BB[over ] pairs produced in e+e- annihilation. The results for the branching fractions are, in units of 10(-6), B(B+-->b1(0)pi+)=6.7+/-1.7+/-1.0, B(B+-->b1(0)K+)=9.1+/-1.7+/-1.0, B(B0-->b1(-/+)pi(+/-))=10.9+/-1.2+/-0.9, and B(B0-->b1(-)K+)=7.4+/-1.0+/-1.0, with the assumption that B(b1-->omega pi)=1. We also measure charge and flavor asymmetries A(ch)(B+-->b1(0)pi+)=0.05+/-0.16+/-0.02, Ach(B+-->b1(0)K+)=-0.46+/-0.20+/-0.02, A(ch)(B0-->b1(-/+)pi(+/-))=-0.05+/-0.10+/-0.02, C(B0-->b1(-/+)pi(+/-))=-0.22+/-0.23+/-0.05, DeltaC(B0-->b1(-/+)pi(+/-))=-1.04+/-0.23+/-0.08, and A(ch)(B0-->b1(-)K+)=-0.07+/-0.12+/-0.02. The first error quoted is statistical, and the second systematic.

  1. Resilience in IMS

    DEFF Research Database (Denmark)

    Kamyod, Chayapol; Nielsen, Rasmus Hjorth; Prasad, Neeli R.

    2012-01-01

    Reliability evaluation of systems has been widely researched for improving system resilience especially in designing processes of a complex system. The convergence of different access networks is possible via IP Multimedia Subsystem (IMS) for development toward Next Generation Networks (NGNs......) and supporting always on services. Therefore, not only Quality of Service (QoS) but also resilience is required. In this paper, we attempt to evaluate and analyze end-to-end reliability of the IMS system using a model proposed as a combination of Reliability Block Diagram (RBD) and Markov Reward Models (MRMs......). The resilience of the IMS architecture is studied by applying 1:1 redundancy at different communication scenarios between end users within and across communication domains. The model analysis provides useful reliability characteristics of the system and can be further applied for system design processes....

  2. IMS-puhelu

    OpenAIRE

    Korba, Kert

    2008-01-01

    Tässä opinnäytetyössä tarkasteltiin IP-Multimediajärjestelmän (IMS) toimintaa ja sen avulla toteutettavan IP-puhelun kulkua. Teoriaosuudessa käsiteltiin myös matkapuhelinverkkojen kehitystä sekä SIP-protokollan toimintaa. Tavoitteena oli toteuttaa IMS-puhelu kahden päätelaitteen välillä, Satakunnan ammattikorkeakoulun GPRS-verkosta oululaista Octopus-testiympäristöä hyödyntäen. Tiedonlähteenä käytettiin Internet-julkaisuja ja kirjallisuutta. The evolution of mobile networks and SIP we...

  3. Delegation und Kooperation im Gesundheitswesen

    OpenAIRE

    Rosenau, Henning

    2010-01-01

    Delegation und Kooperation im Gesundheitswesen. - In: Tıpta işbirliği ve hukuksal sorunlar = Delegation und Kooperation im Gesundheitswesen / ed.: Hakan Hakeri ... - Samsun : Adalet, 2010. - S. 7-18

  4. Aflatoxin B1 in common Egyptian foods.

    Science.gov (United States)

    Selim, M I; Popendorf, W; Ibrahim, M S; el Sharkawy, S; el Kashory, E S

    1996-01-01

    Samples of common Egyptian foods (17 nuts and seeds, 10 spices, 31 herbs and medicinal plants, 12 dried vegetables, and 28 cereal grains) were collected from markets in Cairo and Giza. A portion of each sample was extracted with chloroform, and the concentrated extract was cleaned by passing through a silica gel column. Aflatoxin B1 was determined by reversed-phase liquid chromatography with UV detection. The highest prevalence of aflatoxin B1 was in nuts and seeds (82%), followed by spices (40%), herbs and medicinal plants (29%), dried vegetables (25%), and cereal grains (21%). The highest mean concentration of aflatoxin B1 was in herb and medicinal plants (49 ppb), followed by cereals (36 ppb), spices (25 ppb), nuts and seeds (24 ppb), and dried vegetables (20 ppb). Among nuts and seeds, the prevalence of aflatoxin B1 was highest (100%) in watermelon seeds, inshell peanuts, and unshelled peanuts. The lowest prevalence and concentrations were in hommos (garbanzo beans). The highest concentrations of aflatoxin B1 were detected in foods that had no potential for field contamination but required drying during processing and storage, such as pomegranate peel, watermelon seeds, and molokhia.

  5. Leptin im Knochenstoffwechsel

    Directory of Open Access Journals (Sweden)

    Vock L

    2003-01-01

    Full Text Available Leptin ist ein in Adipozyten exprimiertes Hormon mit vielfältigen Funktionen im gesamten Organismus. Verschiedene Studien legen eine positive Wirkung von Leptin auf den Knochenstoffwechsel nahe. In vitro führte die Inkubation von Knochenmarkstromazellen mit Leptin zu deren Differenzierung zu Osteoblasten und Mineralisation der Matrix. Bei ovarektomierten Ratten konnte durch Leptin der Knochenverlust vermindert werden. Überraschenderweise zeigten andere Studien eine zentrale inhibitorische Wirkung von Leptin auf Osteoblasten bei Nagern. Diese einander scheinbar widersprechenden Ergebnisse zeigen, daß die molekularen Mechanismen, mit welchen Leptin auf den Knochenstoffwechsel wirkt, noch nicht ausreichend verstanden werden. In klinischen Studien findet sich eine protektive Wirkung von Fettmasse und "body mass index" (BMI auf die Knochendichte (postmenopausaler Frauen überwiegend bestätigt. Damit im Einklang, konnten erhöhte Leptinspiegel gefunden werden. Ob Leptin nun direkt in den Knochenstoffwechsel eingreift oder bloß indirekt etwa über eine erhöhte mechanische Belastung, läßt sich aus klinischen Studien allein nicht beantworten. Hingegen dürfte Leptin im Knochenstoffwechsel von Männern keine oder nur eine untergeordnete Rolle spielen. Die Situation bei Kindern und Jugendlichen ist noch unklar, wobei eine positive Wirkung von Leptin in fetalem Knochen relativ sicher ist. Leptin könnte jedoch auch im Wachstum und der Knochenreifung bei Kindern und Jugendlichen eine Rolle spielen.

  6. Uber Phosphorylierung im Licht

    Science.gov (United States)

    Warburg, Otto

    1962-01-01

    Während bisher der Zusammenhang zwischen Dehydrierung und Phosphorylierung im Licht nur für die stöchiometrischen Chinonreactionen bewiesen worden war, ist nunmehr auch für die katalytischen Chinonreactionen, die aeroben wie die anaeroben, gezeigt worden, dass die Dehydrierung die Phosphorylierung bewirkt. Eine andere Phosphorylierung, als die Phosphorylierung durch Dehydrierung gibt es in den grünen Grana nicht. Natürlich entsteht nunmehr die Frage, welche Substanz das Substrat von Dehydrierung und Phosphorylierung ist. Da die Grana keine Dunkelatmung haben und deshalb im Dunkeln nicht phosphorylieren können, auch nicht nach Zusatz von Chinon, so muss das gesuchte Substrat im Licht entstehen; und da der Photolyt der Granareactionen eine Kohlensäureverbindung ist, so muss das gesuchte Substrat eine im Licht entstehende Kohlenstoffverbindung sein. Wegen der Gärungen und dem einzigen bisher bekannten chemischen Mechanismus der Phosphorylierung durch Dehydrierung (4) denkt man an Triosephosphat. Aber chemisch ist jeder Aldehyd möglich. PMID:14004907

  7. IMS Application Developer's Handbook Creating and Deploying Innovative IMS Applications

    CERN Document Server

    Noldus, Rogier; Mulligan, Catherine; Fikouras, Ioannis; Ryde, Anders; Stille, Mats

    2011-01-01

    IMS Application Developer Handbook will give a hands-on view of exactly what needs to be done by IMS application developers to write an application and take it "live" on an operator's network. It will offer practical guidance on building innovative applications using the features and capabilities of the IMS network, show how the rapidly changing development environment is impacting on the business models employed in the industry and how existing network solutions can be moved towards IMS. The book will also elaborate on how IMS applies basic VoIP principles and techniques to realise a true mul

  8. Im Bild sprechen: Cixous

    Directory of Open Access Journals (Sweden)

    Jens E. Sennewald

    2006-03-01

    Full Text Available Gemeinsam von Künstlern und Wissenschaftlern/Philosophen erstellte Bücher sind selten und nicht immer erfolgreich. Eines der Fährnisse ist das Verhältnis zwischen bildlichen und schriftlichen Anteilen: Nicht selten wird der künstlerische Anteil zur Illustration der dominierenden Schrift. Eine Neupositionierung des Bildes im Buch ist nun der amerikanischen Künstlerin Roni Horn gelungen. Mehr als „sujet“ ihres Buches, ist die französische Philosophin Hélène Cixous gleichsam instruierende „Stimme“, die im Bild aufscheint. Horn hat dadurch zugleich – einmal mehr – das Phantasma unmittelbarer Abbildhaftigkeit des Fotos dekonstruiert und der philosophischen Position Cixous‘ durch das Bild eine Dimension hinzugefügt.

  9. "Don Quijote" im Film

    OpenAIRE

    Altenberg, Tilmann

    2007-01-01

    Wie kein zweiter Roman zieht der "Don Quijote" von Miguel de Cervantes seit nunmehr vier Jahrhunderten Generationen von Lesern immer wieder neu in seinen Bann. Abgefasst im Spanien des frühen 17. Jahrhunderts, das zu jener Zeit die Geschicke Europas wesentlich mitbestimmte, wurde der Roman bald zum Inbegriff der spanischen Literatur und Kultur. Von Madrid aus hat er auf den Rest Europas ausgestrahlt und Denker, Dichter, Künstler, Komponisten und später auch Filmemacher zur Auseinandersetzung ...

  10. Sexting im Schulumfeld

    Directory of Open Access Journals (Sweden)

    Barbara Buchegger

    2015-03-01

    Full Text Available Wir wollten es einfach wissen. Wie steht es nun mit diesem "Sexting" – also der Übermittlung von Nacktaufnahmen, die Jugendliche untereinander teilen? Stimmt unser Eindruck aus den Workshops in Schulen, dass es bereits ein "übliches Verhalten" der Jugendlichen geworden ist? Saferinternet.at hat im November/Dezember 2014 eine Studie durch das Institut für Jugendkulturforschung rund um das Thema "Sexting" in Auftrag gegeben.

  11. Energieeffizienz im Rechenzentrum

    Science.gov (United States)

    von Hintemann, Ralph; Skurk, Holger

    In den stark automatisierten, arbeitsteiligen Wirtschaftsystemen der Industrienationen ist die eine effiziente und zuverlässige zentrale Informationstechnik (IT) der Unternehmen entscheidend für den Geschäftserfolg. Immer mehr Unternehmensabläufe werden durch die IT unterstützt. Vielfach ist es sogar nur noch durch die umfassende IT-Unterstützung der Geschäftsprozesse möglich, im globalen Wettbewerb erfolgreich zu sein. Die installierte Rechenleistung in modernen Unternehmen steigt dabei ständig an. Neue und verbesserte Anwendungen und Programm-Features erfordern leistungsfähige Server. In den Rechenzentren kleiner und mittlerer Unternehmen sind heute Rechenleistungen installiert, die vor wenigen Jahren ausschließlich einigen Großunternehmen vorbehalten waren. Diese im Grundsatz positive Entwicklung kann aber auch zu hohen Strom- und Kühlleistungen in Rechenzentren führen. Damit stellen sich neue Herausforderungen an die Planung, Ausführung und den Betrieb einer IT-Infrastruktur.

  12. IMS applications analysis

    Energy Technology Data Exchange (ETDEWEB)

    RODACY,PHILIP J.; REBER,STEPHEN D.; SIMONSON,ROBERT J.; HANCE,BRADLEY G.

    2000-03-01

    This report examines the market potential of a miniature, hand-held Ion Mobility Spectrometer. Military and civilian markets are discussed, as well as applications in a variety of diverse fields. The strengths and weaknesses of competing technologies are discussed. An extensive Ion Mobility Spectrometry (IMS) bibliography is included. The conclusions drawn from this study are: (1) There are a number of competing technologies that are capable of detecting explosives, drugs, biological, or chemical agents. The IMS system currently represents the best available compromise regarding sensitivity, specificity, and portability. (2) The military market is not as large as the commercial market, but the military services are more likely to invest R and D funds in the system. (3) Military applications should be addressed before commercial applications are addressed. (4) There is potentially a large commercial market for rugged, hand-held Ion Mobility Spectrometer systems. Commercial users typically do not invest R and D funds in this type of equipment rather, they wait for off-the-shelf availability.

  13. Wortstellungsforschungen im Altgriechischen

    Directory of Open Access Journals (Sweden)

    Matjaž Babič

    2003-07-01

    Full Text Available Wenn man heutzutage Wortstellungsforschungen in einer Korpussprache unternimmt, beklagt man oft die Schwierigkeiten einer derartigen Arbeit. Im Falle des Altgriechischen hätte man eigentlich wenig zu beklagen, denn es mangelt nicht an Material, dazu sind die Texte, die uns zur Verfügung stehen, stilistisch und funktionell verschiedenartig. Es gibt mehrere Gründe, Wortstellung im Altgriechischen zu untersuchen: stilistische Eigenschaften, das Neugriechische sowie das Verhältnis des Altgriechischen zum Indogermanischen. Es liegt zwar auf der Hand, die Untersuchung auf typologischer Grundlage durchzuführen, die Feststellungen, zu denen man auf diese Weiße gelangt, sind jedoch selten schlüssig. Es ist demnach alles andere als leicht, mit solchen Forschungen zuverlässige Ergebnisse zu erzielen. Die erhaltenen Texte können einen 'native speaker' selbstverständlich nicht ersetzen, zumal die griechischen Grammatiker die Syntax oft bei Seite ließen und die Wortstellungsvarianten der Lehre über den Stil überließen. Ein derartiges Beispiel bieten uns Aristoteles' Poetik und die Abhandlung De compositione verborum des Dionysios von Halikarnaß.Es dürfte uns also kaum überraschen, wenn die Forschungen des 19. und 20. Jahrhunderts sich großenteils mit der Methodologie befaßten.

  14. 118-B-1 excavation treatability test procedures

    International Nuclear Information System (INIS)

    Frain, J.M.

    1994-01-01

    This treatability study has two purposes: to support development of the approach to be used for burial ground remediation, and to provide specific engineering information for the design of burial grounds receiving waste generated from the 100 Area removal actions. Data generated from this test will also provide performance and cost information necessary for detailed analysis of alternatives for burial ground remediation. Further details on the test requirements, milestones and data quality objectives are described in detail in the 118-B-1 Excavation Treatability Test Plan (DOE/RL-94-43). These working procedures are intended for use by field personnel to implement the requirements of the milestone. A copy of the detailed Test Plan will be kept on file at the on-site field support trailer, and will be available for review by field personnel

  15. 118-B-1 excavation treatability test plan

    International Nuclear Information System (INIS)

    1994-07-01

    The Hanford 118-B-1 Burial Ground Treatability Study has been required by milestone change request number-sign M-15-93-04, dated September 30, 1993. The change request requires that a treatability test be conducted at the 100-B Area to obtain additional engineering information for remedial design of burial grounds receiving waste from 100 Area removal actions. This treatability study has two purposes: (1) to support development of the Proposed Plan (PP) and Record of Decision (ROD), which will identify the approach to be used for burial ground remediation, and (2) to provide specific engineering information for receiving waste generated from the 100 Area removal actions. Data generated from this test also will provide critical performance and cost information necessary for remedy evaluation in the detailed analysis of alternatives during preparation of the focused feasibility study (FFS). This treatability testing supports the following 100 Area alternatives: (1) excavation and disposal, and (2) excavation, sorting, (treatment), and disposal

  16. 118-B-1 excavation treatability test procedures

    Energy Technology Data Exchange (ETDEWEB)

    Frain, J.M.

    1994-08-01

    This treatability study has two purposes: to support development of the approach to be used for burial ground remediation, and to provide specific engineering information for the design of burial grounds receiving waste generated from the 100 Area removal actions. Data generated from this test will also provide performance and cost information necessary for detailed analysis of alternatives for burial ground remediation. Further details on the test requirements, milestones and data quality objectives are described in detail in the 118-B-1 Excavation Treatability Test Plan (DOE/RL-94-43). These working procedures are intended for use by field personnel to implement the requirements of the milestone. A copy of the detailed Test Plan will be kept on file at the on-site field support trailer, and will be available for review by field personnel.

  17. Effects of prolonged oral administration of fumonisin B1 and aflatoxin B1 in rats.

    Science.gov (United States)

    Pozzi, C R; Corrêa, B; Xavier, J G; Direito, G M; Orsi, R B; Matarazzo, S V

    2001-01-01

    The effects of prolonged oral administration (21 days) of fumonisin B1 (FB1) and aflatoxin B1 (AFB1) were evaluated on male Wistar rats. The animals were housed in individual metabolic cages and submitted to the following treatments: 1-0 microg AFB1 + 0 mg FB1/100g bw.; 2-72 microg AFB1+ 0 mg FB1/100 g bw; 3-0 microg AFB1 + 0.5 mg FB1 g bw; 4-0 microg AFB1 + 1.5 mg FB1/100 g bw; 5-72 microg AFB1 + 0.5 mg FB1/100g bw; 6-72 microgAFB1 + 1.5 mg FB1/100g bw. On day 21, the rats were sacrificed for evaluation. The results showed that treated animals presented differences in body weight and absolute/relative weights of liver and kidney as well as altered hepatic function and cholesterol blood levels. Rats fed with the greatest doses of AFB1 and FB1 gained less weight (2.79 g/day) at the end of the experimental period; their blood concentrations of liver enzymes aspartate aminotransferase (AST) and alkaline phosphatase (AP) were above control levels (130.35 micro/l and 471.00 micro/l, respectively). Blood cholesterol increased in the groups treated with the highest dose of FB1 or FB1 associated with AFB1. Histopathology revealed the occurrence of apoptosis in the liver of rats exposed to FB1. The association of aflatoxin B1 with fumonisin B1 at higher dose probably potentiated the effects of the higher dose of fumonisin B1 acting singly.

  18. CD25+ B-1a Cells Express Aicda

    Directory of Open Access Journals (Sweden)

    Hiroaki Kaku

    2017-06-01

    Full Text Available B-1a cells are innate-like B-lymphocytes producing natural antibodies. Activation-induced cytidine deaminase (AID, a product of the Aicda gene, plays a central role in class-switch recombination and somatic hypermutation in B cells. Although a role for Aicda in B-1a cells has been suggested on the basis of experiments with knock out (KO mice, whether B-1a cells express Aicda, and if so, which B-1a cell subpopulation expresses Aicda, remains unknown. Here, we demonstrate that B-1 cells express Aicda, but at a level below that expressed by germinal center (GC B cells. We previously reported that B-1a cells can be subdivided based on CD25 expression. We show here that B-1a cell Aicda expression is concentrated in the CD25+ B-1a cell subpopulation. These results suggest the possibility that previous studies of memory B cells identified on the basis of Aicda expression may have inadvertently included an unknown number of CD25+ B-1a cells. Although B-1a cells develop normally in the absence of Aicda, a competitive reconstitution assay reveals enhanced vigor for AID KO B-1a cell bone marrow (BM progenitors, as compared with wild-type BM B-1 cell progenitors. These results suggest that AID inhibits the development of B-1a cells from BM B-1 cell progenitors in a competitive environment.

  19. Jung und Alt im Dialog

    Directory of Open Access Journals (Sweden)

    Caroline Baetge

    2012-04-01

    Full Text Available Rezension zu: Kupser, Thomas, und Ida Pöttinger, Hrsg. 2011. Mediale Brücken: Generationen im Dialog durch aktive Medienarbeit. Gesellschaft - Altern - Medien 3. München: kopaed.

  20. Gene Directed Enzyme Prodrug Therapy Using Rabbit Cytochrome P450 4B1 in Murine Colon Adenocarcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Sung Joo; Kang, Joo Hyun; Lee, Tae Sup; Kim, Kyeong Min; Woo, Kwang Sun; Chung, Wee Sup; Cheon, Gi Jeong; Choi, Chang Woon; Lim, Sang Moo [Korea Institute of Radiological and Medical Sciences, Seoul (Korea, Republic of)

    2007-07-01

    The conventional cancer therapy is chemotherapy, surgical resection and/or radiotherapy. Chemotherapy using cytotoxic drug has some problems with lack of tumor selectivity resulting in toxicity to normal tissues. To enhance the tumor selectivity of cytotoxic drug, the application of suicidal gene therapy technology was designed. Suicidal gene therapy is based on the expression in tumor cells of a gene encoding an enzyme that converts a non-toxic prodrug into a cytotoxic product. Representative suicidal genes are Herpes simplex virus type 1 thymidine kinase (HSV1- tk) and cytosine deaminase (cd). Recently, a new prodrug-converting enzyme based on rabbit cytochrome P450 4B1 gene (cyp4B1) has been reported for therapy of experimental brain tumor. This enzyme activates the prodrugs such as 4-ipomeanol (4-IM) and 2- aminoanthracene (2-AA) to highly reactive furane epoxide and unsaturated dialdehyde intermediate, respectively. DNA alkylation seems to be the main mechanism of cytotoxicity of these activated drugs. In this study, we isolated cyp4B1 cDNA from rabbit lung, transduced cyp4B1 expression vector into murine colon cancer cell, and then analyzed the cytotoxic properties of cyp4b1-activated 2-AA in cyp4B1 transduced cells to verify the cyp4B1 enzyme system for gene directed enzyme prodrug therapy.

  1. Gene Directed Enzyme Prodrug Therapy Using Rabbit Cytochrome P450 4B1 in Murine Colon Adenocarcinoma

    International Nuclear Information System (INIS)

    Kim, Sung Joo; Kang, Joo Hyun; Lee, Tae Sup; Kim, Kyeong Min; Woo, Kwang Sun; Chung, Wee Sup; Cheon, Gi Jeong; Choi, Chang Woon; Lim, Sang Moo

    2007-01-01

    The conventional cancer therapy is chemotherapy, surgical resection and/or radiotherapy. Chemotherapy using cytotoxic drug has some problems with lack of tumor selectivity resulting in toxicity to normal tissues. To enhance the tumor selectivity of cytotoxic drug, the application of suicidal gene therapy technology was designed. Suicidal gene therapy is based on the expression in tumor cells of a gene encoding an enzyme that converts a non-toxic prodrug into a cytotoxic product. Representative suicidal genes are Herpes simplex virus type 1 thymidine kinase (HSV1- tk) and cytosine deaminase (cd). Recently, a new prodrug-converting enzyme based on rabbit cytochrome P450 4B1 gene (cyp4B1) has been reported for therapy of experimental brain tumor. This enzyme activates the prodrugs such as 4-ipomeanol (4-IM) and 2- aminoanthracene (2-AA) to highly reactive furane epoxide and unsaturated dialdehyde intermediate, respectively. DNA alkylation seems to be the main mechanism of cytotoxicity of these activated drugs. In this study, we isolated cyp4B1 cDNA from rabbit lung, transduced cyp4B1 expression vector into murine colon cancer cell, and then analyzed the cytotoxic properties of cyp4b1-activated 2-AA in cyp4B1 transduced cells to verify the cyp4B1 enzyme system for gene directed enzyme prodrug therapy

  2. Resilience of the IMS system

    DEFF Research Database (Denmark)

    Kamyod, Chayapol; Nielsen, Rasmus Hjorth; Prasad, Neeli R.

    2014-01-01

    The paper focuses on end-to-end resilience analysis of the IMS based network through the principal resilience parameters by using OPNET. The resilience behaviours of communication across multiple IMS domains are investigated at different communication scenarios and compared with previous state......-of-the-art. Moreover, the resilience effects when adding a redundancy of the S-CSCF unit are examined. The results disclose interesting resilience behaviours for long distance communications....

  3. 26 CFR 1.267(b)-1 - Relationships.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Relationships. 1.267(b)-1 Section 1.267(b)-1...) INCOME TAXES Items Not Deductible § 1.267(b)-1 Relationships. (a) In general. (1) The persons referred to... partnership separately. Therefore, if the other person and a partner are within any one of the relationships...

  4. Cellular consequences of ATP8B1 deficiency

    NARCIS (Netherlands)

    van der Velden, L.M.

    2010-01-01

    Mutations in the ATP8B1 gene cause a spectrum of familial intrahepatic cholestasis syndromes which we collectively refer to as ATP8B1 deficiency. Patients with ATP8B1 deficiency present with intrahepatic cholestasis (impairment of bile flow) as primary complication. These patients may also present

  5. 49 CFR 178.33b-1 - Compliance.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Compliance. 178.33b-1 Section 178.33b-1 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY... Specifications for Inside Containers, and Linings § 178.33b-1 Compliance. (a) Required in all details. (b...

  6. 26 CFR 301.7701(b)-1 - Resident alien.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Resident alien. 301.7701(b)-1 Section 301.7701... ADMINISTRATION PROCEDURE AND ADMINISTRATION Definitions § 301.7701(b)-1 Resident alien. (a) Scope. Section 301.7701(b)-1(b) provides rules for determining whether an alien individual is a lawful permanent resident...

  7. Compound list: aflatoxin B1 [Open TG-GATEs

    Lifescience Database Archive (English)

    Full Text Available aflatoxin B1 AFB1 00165 ftp://ftp.biosciencedbc.jp/archive/open-tggates/LATEST/Human/in_vitro/aflatoxin..._B1.Human.in_vitro.Liver.zip ftp://ftp.biosciencedbc.jp/archive/open-tggates/LATEST/Rat/in_vivo/Liver/Single/aflatoxin_B1.Rat.in_vivo.Liver.Single.zip ...

  8. Literatuuroverzicht analysemethodes voor vitamine B1 en B2

    NARCIS (Netherlands)

    Oostenbrink, T.; Hollman, P.

    1982-01-01

    Inventarisatie analysemethodes vitamine B1 en B2. De extraktie van vitamine B1 en B2 dient voor een aantal levensmiddelen nader onderzocht te worden. Het is zinvol een automatische methode voor vitamine B1 en B2 op te starten. HPLC methodes zijn voor de levensmiddelenanalyse nog in een beginstadium.

  9. An IMS-IMS Analogue of MS-MS

    Energy Technology Data Exchange (ETDEWEB)

    Koeniger, Stormy L.; Merenbloom, Samuel I.; Valentine, Stephen J.; Jarrold, Martin F.; Udseth, Harold R.; Smith, Richard D.; Clemmer, David E.

    2006-06-15

    The development of a new ion mobility/mass spectrometry instrument that incorporates a multifield drift tube/ion funnel design is described. In this instrument, individual components from a mixture of ions can be resolved and selected on the basis of mobility differences prior to collisional activation inside the drift tube. The fragment ions that are produced can be dispersed again in a second ion mobility spectrometry (IMS) region prior to additional collisional activation and MS analysis. The result is an IMS-IMS analogue of MS-MS. Here, we describe the preliminary instrumental design and experimental approach. We illustrate the approach by examining the highly characterized bradykinin and ubiquitin systems. Mobility-resolved fragment ions of bradykinin show that b-type ions are readily discernible fragments, because they exist as two easily resolvable structural types. Current limitations and future directions are briefly discussed.

  10. Effects of oral administration of aflatoxin B1 and fumonisin B1 in rabbits (Oryctolagus cuniculus).

    Science.gov (United States)

    Orsi, R B; Oliveira, C A F; Dilkin, P; Xavier, J G; Direito, G M; Corrêa, B

    2007-12-15

    The effects of prolonged oral administration (21 days) of fumonisin B(1) (FB(1)) and aflatoxin B(1) (AFB(1)) were studied in male New Zealand rabbits by clinical, pathological, biochemical and sphingolipid analyses. Twenty-four animals were randomly divided into the following four experimental groups: (A) 0 mg FB(1)+0 microg AFB(1)/(kg body weight(bw)day) (control); (B) 0 mg FB(1)+30 microg AFB(1)/(kg bw day); (C) 1.5 mg FB(1)/(kg bw day)+30 microg AFB(1)/(kg bw day); (D) 1.5 mg FB(1)/(kg bw day)+0 microg AFB(1). Animals from group B and principally from group C presented clinical signs of intoxication. Rabbits from group C presented a lower body weight gain than controls. Differences were observed between intoxicated rabbits and controls with respect to absolute and relative liver and kidney weight, hepatic function, serum urea and creatinine levels and Sa/So ratio. The most frequent hepatic and renal injuries were vacuolar degeneration of the liver and kidney as shown by the histopathological and serum biochemical results. Combined administration of AFB(1) and FB(1) resulted in synergistic toxic effects both in the liver and in the kidney, but hepatic injuries were more marked.

  11. Expression and Initial Characterization of Human ALDH3B1

    OpenAIRE

    Marchitti, Satori A.; Orlicky, David J.; Vasiliou, Vasilis

    2007-01-01

    Aldehyde dehydrogenases (ALDHs) are critical enzymes in the metabolism of endogenous and exogenous aldehydes. The human genome contains nineteen putatively functional ALDH genes; ALDH3B1 belongs to the ALDH3 family. While recent studies have linked the ALDH3B1 locus to schizophrenia, nothing was known, until now, about the properties and significance of the ALDH3B1 protein. The aim of this study was to characterize the ALDH3B1 protein. Human ALDH3B1 was baculovirus-expressed and found to be c...

  12. Pulsation research during the IMS

    Energy Technology Data Exchange (ETDEWEB)

    Hughes, W.J.

    1982-08-01

    Workers in the pulsation field entered the period of the International Magnetospheric Study (IMS) with a lot of theories that needed testing. Because of this and the wealth of new data available from the IMS observational campaigns, many new observational results have emerged from the IMS. The experience of the IMS shows us that the most fruitful method of working has been small-scale collaborations, between either a few individuals or a few groups with complementary data sets. The larger, more formally organized workshops do not seem to have been as successful in producing results, though they may have fostered useful contacts. In the years to come I hope and expect to see more of this sort of small-scale collaboration; especially as the initial results obtainable from a single data set are now published, experiments should turn to seeing how their data can be used in conjunction with other data sets. I also feel that the time is ripe for more theoretical work. The IMS has left us with a lot of new observations that need explanation.

  13. Wozu und wie kooperative Arbeitsformen im studienbegleitenden Deutschunterricht?

    Directory of Open Access Journals (Sweden)

    Karmelka Barić

    2016-04-01

    Full Text Available Ausgehend von einer Reflexion über die im Beruf und im Leben notwendigen Kompetenzen in der Kommunikation sowie von der Überzeugung, dass es notwendig ist, diese Kompetenzen schon im Studium durch geeignete Arbeitsverfahren und Themen zu entwickeln, werden Kooperation und Autonomie fördernde Arbeitsformen für den studienbegleitenden Deutsch- und Fremdsprachenunterricht (SDU/SFU vorgestellt. Diese Arbeitsformen sind auf andere Sprachen übertragbar und aus dem Lehrwerk Mit Deutsch studieren arbeiten leben A2/B1 (Lévy-Hillerich, Serena, Barić & Cickovska 2010 und der dazu gehörenden Lernplattform entnommen. Das Lehrwerk selbst ist eine Umsetzung der SDU-Rahmencurricula, die im Laufe von über zwanzig Jahren in zehn Ländern im Rahmen eines 1994 am Goethe-Institut Warschau begonnenen Hochschulprojekts und der daraus entstandenen Forschungstätigkeit entstanden sind. Grundlage des Projekts ist ein ganzheitlicher soziopädagogischer Ansatz, der LernerInnen als Menschen betrachtet, die im Präsenz- oder im virtuellen Unterricht in der Sprache und durch die Sprache fach- und sprachübergreifende Kooperationsfähigkeiten entwickeln, die sich später im Leben in der Gesellschaft auswirken. Nach der Vorstellung des Projekts und seiner wissenschaftlichen Hintergründe im ersten Teil des Beitrags werden im zweiten Teil die sich aus dem Ansatz ergebenden Arbeitsformen untersucht: Dabei werden die sich dazu eignenden Themen, die Lernhilfen für StudentInnen, die den LehrerInnen zufallenden Rollen sowie die Ansprüche an Lehrbücher/Unterrichtsmaterialien und Online-/Blended-Learning-Lernszenarien besprochen. Der abschließende Überblick im dritten Teil über die Schwierigkeiten, die beim kooperativen Lernen im SDU/SFU auftreten können, ist gleichzeitig eine offenbleibende Frage danach, wie LehrerInnen aus- und fortgebildet werden sollten, um sich den Anforderungen einer sich dauernd verändernden Lernlandschaft stellen zu können.   The paper

  14. B1 -sensitivity analysis of quantitative magnetization transfer imaging.

    Science.gov (United States)

    Boudreau, Mathieu; Stikov, Nikola; Pike, G Bruce

    2018-01-01

    To evaluate the sensitivity of quantitative magnetization transfer (qMT) fitted parameters to B 1 inaccuracies, focusing on the difference between two categories of T 1 mapping techniques: B 1 -independent and B 1 -dependent. The B 1 -sensitivity of qMT was investigated and compared using two T 1 measurement methods: inversion recovery (IR) (B 1 -independent) and variable flip angle (VFA), B 1 -dependent). The study was separated into four stages: 1) numerical simulations, 2) sensitivity analysis of the Z-spectra, 3) healthy subjects at 3T, and 4) comparison using three different B 1 imaging techniques. For typical B 1 variations in the brain at 3T (±30%), the simulations resulted in errors of the pool-size ratio (F) ranging from -3% to 7% for VFA, and -40% to > 100% for IR, agreeing with the Z-spectra sensitivity analysis. In healthy subjects, pooled whole-brain Pearson correlation coefficients for F (comparing measured double angle and nominal flip angle B 1 maps) were ρ = 0.97/0.81 for VFA/IR. This work describes the B 1 -sensitivity characteristics of qMT, demonstrating that it varies substantially on the B 1 -dependency of the T 1 mapping method. Particularly, the pool-size ratio is more robust against B 1 inaccuracies if VFA T 1 mapping is used, so much so that B 1 mapping could be omitted without substantially biasing F. Magn Reson Med 79:276-285, 2018. © 2017 International Society for Magnetic Resonance in Medicine. © 2017 International Society for Magnetic Resonance in Medicine.

  15. Individualisierte Produkte im Fokus der intergrierten Produktentwicklung

    OpenAIRE

    Baumberger, C.;Gahr, A.

    2017-01-01

    Individualisierte Produkte stellen neue Herausforderungen an die Produktentwicklung – vornehmlich im Bereich der Produktstrukturplanung, der kundenindividuellen Produktadaption und dem Zielkostenmanagement. Diese Themen werden am Lehrstuhl für Produktentwicklung im Rahmen des Sonderforschungsbereiches 582 behandelt.

  16. 29 CFR 2530.200b-1 - Computation periods.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Computation periods. 2530.200b-1 Section 2530.200b-1 Labor... Provisions § 2530.200b-1 Computation periods. (a) General. Under sections 202, 203 and 204 of the Act and... specified period—in general, a twelve-consecutive-month period—referred to herein as a “computation period...

  17. MAN1B1 deficiency: an unexpected CDG-II.

    Directory of Open Access Journals (Sweden)

    Daisy Rymen

    Full Text Available Congenital disorders of glycosylation (CDG are a group of rare metabolic diseases, due to impaired protein and lipid glycosylation. In the present study, exome sequencing was used to identify MAN1B1 as the culprit gene in an unsolved CDG-II patient. Subsequently, 6 additional cases with MAN1B1-CDG were found. All individuals presented slight facial dysmorphism, psychomotor retardation and truncal obesity. Generally, MAN1B1 is believed to be an ER resident alpha-1,2-mannosidase acting as a key factor in glycoprotein quality control by targeting misfolded proteins for ER-associated degradation (ERAD. However, recent studies indicated a Golgi localization of the endogenous MAN1B1, suggesting a more complex role for MAN1B1 in quality control. We were able to confirm that MAN1B1 is indeed localized to the Golgi complex instead of the ER. Furthermore, we observed an altered Golgi morphology in all patients' cells, with marked dilatation and fragmentation. We hypothesize that part of the phenotype is associated to this Golgi disruption. In conclusion, we linked mutations in MAN1B1 to a Golgi glycosylation disorder. Additionally, our results support the recent findings on MAN1B1 localization. However, more work is needed to pinpoint the exact function of MAN1B1 in glycoprotein quality control, and to understand the pathophysiology of its deficiency.

  18. Advanced Interval Management (IM) Concepts of Operations

    Science.gov (United States)

    Barmore, Bryan E.; Ahmad, Nash'at N.; Underwood, Matthew C.

    2014-01-01

    This document provides a high-level description of several advanced IM operations that NASA is considering for future research and development. It covers two versions of IM-CSPO and IM with Wake Mitigation. These are preliminary descriptions to support an initial benefits analysis

  19. 23 CFR 500.111 - IMS.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false IMS. 500.111 Section 500.111 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION TRANSPORTATION INFRASTRUCTURE MANAGEMENT MANAGEMENT AND MONITORING SYSTEMS Management Systems § 500.111 IMS. An effective IMS for intermodal facilities and systems provides efficient, safe, and convenient...

  20. Determination of aflatoxin B1 in food products in Thailand ...

    African Journals Online (AJOL)

    Aflatoxin B1 is generally found in feed and food stuff, such as cereal and all products derived from cereals, including processed cereals since it has been proven to be at least partly resistant to food processing methods. Hence, the aim of this study was to determine the possibility of contamination of aflatoxin B1 in food ...

  1. Fumonisin B 1 Reduction in Lactic Acid Bacteria Fermentation of ...

    African Journals Online (AJOL)

    This study investigated how fermentation can promote fumonisin B1 reduction in maize-based porridges. Four starter culture of lactic acid bacteria (Lactobacillus plantarum, Pediococcus pentosaceus, Lactobacillus casei and Lactobacillus fermentum) were used, porridge samples was spiked with fumonisins B1 and allowed ...

  2. SerpinB1 Promotes Pancreatic β Cell Proliferation

    Energy Technology Data Exchange (ETDEWEB)

    El Ouaamari, Abdelfattah; Dirice, Ercument; Gedeon, Nicholas; Hu, Jiang; Zhou, Jian-Ying; Shirakawa, Jun; Hou, Lifei; Goodman, Jessica; Karampelias, Christos; Qiang, Guifeng; Boucher, Jeremie; Martinez, Rachael; Gritsenko, Marina A.; De Jesus, Dario F.; Kahraman, Sevim; Bhatt, Shweta; Smith, Richard D.; Beer, Hans-Dietmar; Jungtrakoon, Prapaporn; Gong, Yanping; Goldfine, Allison B.; Liew, Chong Wee; Doria, Alessandro; Andersson, Olov; Qian, Wei-Jun; Remold-O’Donnell, Eileen; Kulkarni, Rohit N.

    2016-01-01

    Compensatory β-cell growth in response to insulin resistance is a common feature in diabetes. We recently reported that liver-derived factors participate in this compensatory response in the liver insulin receptor knockout (LIRKO) mouse, a model of significant islet hyperplasia. Here we show that serpinB1 is a liver-derived secretory protein that controls β-cell proliferation. SerpinB1 is abundant in the hepatocyte secretome and sera derived from LIRKO mice. SerpinB1 and small molecule compounds that partially mimic serpinB1 activity enhanced proliferation of zebrafish, mouse and human β-cells. We report that serpinB1-induced β-cell replication requires protease inhibition activity and mice lacking serpinB1 exhibit attenuated β-cell replication in response to insulin resistance. Finally, SerpinB1-treatment of islets modulated signaling proteins in growth and survival pathways such as MAPK, PKA and GSK3. Together, these data implicate SerpinB1 as a protein that can potentially be harnessed to enhance functional β-cell mass in patients with diabetes.

  3. 77 FR 51780 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2012-08-27

    ... of the Secretary 36(b)(1) Arms Sales Notification AGENCY: Department of Defense, Defense Security... a section 36(b)(1) arms sales notification. This is published to fulfill the requirements of section..., and Airfield Lighting System, spare and repair parts, support equipment, personnel training and...

  4. 78 FR 15004 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2013-03-08

    ... of the Secretary 36(b)(1) Arms Sales Notification AGENCY: Defense Security Cooperation Agency... of a section 36(b)(1) arms sales notification. This is published to fulfill the requirements of..., support equipment, personnel training and training equipment, Quality Assurance Team, tools and test...

  5. 77 FR 40026 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2012-07-06

    ... of the Secretary 36(b)(1) Arms Sales Notification AGENCY: Department of Defense, Defense Security... a section 36(b)(1) arms sales notification. This is published to fulfill the requirements of section... test equipment, repair and return support, training equipment and personnel training, U.S. Government...

  6. 77 FR 65185 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2012-10-25

    ... of the Secretary 36(b)(1) Arms Sales Notification AGENCY: Department of Defense, Defense Security... a section 36(b)(1) arms sales notification. This is published to fulfill the requirements of section...-9X-2 Captive Air Training Missiles, 2 AIM- 9X-2 NATM Special Air Training Missiles, 2 CATM-9X-2 Block...

  7. 77 FR 53180 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2012-08-31

    ... of the Secretary 36(b)(1) Arms Sales Notification AGENCY: Department of Defense, Defense Security... a section 36(b)(1) arms sales notification. ] This is published to fulfill the requirements of...; support equipment; spare and repair parts; repair and return; personnel training and training equipment; U...

  8. 77 FR 49434 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2012-08-16

    ... of the Secretary 36(b)(1) Arms Sales Notification AGENCY: Department of Defense, Defense Security... a section 36(b)(1) arms sales notification. This is published to fulfill the requirements of section..., support equipment, tools and test equipment, technical data and publications, personnel training and...

  9. 76 FR 60461 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2011-09-29

    ... of the Secretary 36(b)(1) Arms Sales Notification AGENCY: Department of Defense, Defense Security... a section 36(b)(1) arms sales notification. This is published to fulfill the requirements of section..., repair and return support, training equipment and personnel training, U.S. Government and contractor...

  10. THOR Ion Mass Spectrometer (IMS)

    Science.gov (United States)

    Retinò, Alessandro

    2017-04-01

    Turbulence Heating ObserveR (THOR) is the first mission ever flown in space dedicated to plasma turbulence. The Ion Mass Spectrometer (IMS) onboard THOR will provide the first high-time resolution measurements of mass-resolved ions in near-Earth space, focusing on hot ions in the foreshock, shock and magnetosheath turbulent regions. These measurements are required to study how kinetic-scale turbulent fluctuations heat and accelerate different ion species. IMS will measure the full three-dimensional distribution functions of main ion species (H+, He++, O+) in the energy range 10 eV/q to 30 keV/q with energy resolution DE/E down to 10% and angular resolution down to 11.25˚ . The time resolution will be 150 ms for O+, 300 ms for He++ and ˜ 1s for O+, which correspond to ion scales in the the foreshock, shock and magnetosheath regions. Such high time resolution is achieved by mounting four identical IMS units phased by 90˚ in the spacecraft spin plane. Each IMS unit combines a top-hat electrostatic analyzer with deflectors at the entrance together with a time-of-flight section to perform mass selection. Adequate mass-per-charge resolution (M/q)/(ΔM/q) (≥ 8 for He++ and ≥ 3 for O+) is obtained through a 6 cm long Time-of-Flight (TOF) section. IMS electronics includes a fast sweeping high voltage board that is required to make measurements at high cadence. Ion detection includes Micro Channel Plates (MCPs) combined with Application-Specific Integrated Circuits (ASICs) for charge amplification and discrimination and a discrete Time-to-Amplitude Converter (TAC) to determine the ion time of flight. A processor board will be used to for ion events formatting and will interface with the Particle Processing Unit (PPU), which will perform data processing for THOR particle detectors. The IMS instrument is being designed and will be built and calibrated by an international consortium of scientific institutes from France, USA, Germany and Japan and Switzerland.

  11. Using IMS VDEX in Agrega

    Directory of Open Access Journals (Sweden)

    Jose Manuel Canabal

    2009-03-01

    Full Text Available An essential element in a learning object repository is the meta-information associated with the resources housed by the repository, as this is what enables the objects to be retrieved. In many cases, the meta-information which is described consists of the classification of a resource in relation to a taxonomy or thesaurus. Agrega is a network of learning object repositories in which an LOM profile, called LOM-ES, has been used to label the objects. Part of the meta-information described corresponds to the classification of the object with respect to a group of taxonomies and a thesaurus, which have had to be described using the ims vdex standard to enable them to be managed from the federation nodes. This article describes the way in which ims vdex has been used in order to create this description and how these metadata instances are managed.

  12. Mathematik im Chip-Design

    Science.gov (United States)

    Koehl, Jürgen; Korte, Bernhard; Vygen, Jens

    Chips sind die wohl komplexesten Strukturen, die vom Menschen entworfen und gefertigt wurden. Auf einem kleinen Silizium-Chip von der Größe eines Fingernagels werden heute Milliarden von Transistoren untergebracht, die mit vielen Millionen Verbindungen untereinander verknüpft sind, wobei die Gesamtlänge dieser Netze mehrere Kilometer betragen kann. Abb. 1 zeigt einen kleinen Ausschnitt (˜ 1 Milliardstel) eines Chips mit zweilagiger Verdrahtung im Raster-Tunnel-Mikroskop.

  13. Der kollektive Kulturschock im Postkommunismus

    OpenAIRE

    Feichtinger, Claudia; Fink, Gerhard

    1999-01-01

    Das Konzept des individuellen Kulturschocks ist bekannt. Es beschreibt psychische und mitunter auch physische Belastungen während des Aufenthalts in einer fremden Kultur, die durch die Konfrontation zwischen eigener und fremder Kultur ausgelöst werden. Nach der Meinung der Autoren gibt es Symptome eines Kulturschocks auch auf der kollektiven (gesellschaftlichen) Ebene. Dieser kollektive Kulturschock löst bestimmte Verhaltensweisen aus, die im Management und in den wirtschaftlichen Beziehungen...

  14. Tvorba IMS aplikací

    OpenAIRE

    Nagy, Ľuboš

    2009-01-01

    Diplomová práca sa zaoberá základnou štúdiou architektúry IMS a možnosťami vývoju aplikácie v nej. V prvej časti práce je popísaná generická architektúra IMS ako vrstvový model rozdelený na štyri logické vrstvy s popisom rozhraní a hlavných entít. Ďalej je znázornená a vysvetlená architektúra SIP protokolu s jej prvkami, ktorý je hlavným signalizačným protokolom v IMS. Vzhžadom na povahu zadania v prípade SIP protokolu sú popísané aj jeho metódy. V poslednej teoretickej časti práce sú spomenu...

  15. Properties of L=1 B1 and B2* Mesons

    Science.gov (United States)

    Abazov, V. M.; Abbott, B.; Abolins, M.; Acharya, B. S.; Adams, M.; Adams, T.; Aguilo, E.; Ahn, S. H.; Ahsan, M.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Alverson, G.; Alves, G. A.; Anastasoaie, M.; Ancu, L. S.; Andeen, T.; Anderson, S.; Andrieu, B.; Anzelc, M. S.; Arnoud, Y.; Arov, M.; Arthaud, M.; Askew, A.; Åsman, B.; Assis Jesus, A. C. S.; Atramentov, O.; Autermann, C.; Avila, C.; Ay, C.; Badaud, F.; Baden, A.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Banerjee, P.; Barberis, E.; Barfuss, A.-F.; Bargassa, P.; Baringer, P.; Barreto, J.; Bartlett, J. F.; Bassler, U.; Bauer, D.; Beale, S.; Bean, A.; Begalli, M.; Begel, M.; Belanger-Champagne, C.; Bellantoni, L.; Bellavance, A.; Benitez, J. A.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Berntzon, L.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bezzubov, V. A.; Bhat, P. C.; Bhatnagar, V.; Biscarat, C.; Blazey, G.; Blekman, F.; Blessing, S.; Bloch, D.; Bloom, K.; Boehnlein, A.; Boline, D.; Bolton, T. A.; Borissov, G.; Bos, K.; Bose, T.; Brandt, A.; Brock, R.; Brooijmans, G.; Bross, A.; Brown, D.; Buchanan, N. J.; Buchholz, D.; Buehler, M.; Buescher, V.; Burdin, S.; Burke, S.; Burnett, T. H.; Buszello, C. P.; Butler, J. M.; Calfayan, P.; Calvet, S.; Cammin, J.; Caron, S.; Carvalho, W.; Casey, B. C. K.; Cason, N. M.; Castilla-Valdez, H.; Chakrabarti, S.; Chakraborty, D.; Chan, K. M.; Chan, K.; Chandra, A.; Charles, F.; Cheu, E.; Chevallier, F.; Cho, D. K.; Choi, S.; Choudhary, B.; Christofek, L.; Christoudias, T.; Cihangir, S.; Claes, D.; Clément, C.; Clément, B.; Coadou, Y.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M.-C.; Crépé-Renaudin, S.; Cutts, D.; Ćwiok, M.; da Motta, H.; Das, A.; Davies, G.; de, K.; de Jong, S. J.; de Jong, P.; de La Cruz-Burelo, E.; Martins, C. De Oliveira; Degenhardt, J. D.; Déliot, F.; Demarteau, M.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Diehl, H. T.; Diesburg, M.; Dominguez, A.; Dong, H.; Dudko, L. V.; Duflot, L.; Dugad, S. R.; Duggan, D.; Duperrin, A.; Dyer, J.; Dyshkant, A.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Eno, S.; Ermolov, P.; Evans, H.; Evdokimov, A.; Evdokimov, V. N.; Ferapontov, A. V.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Ford, M.; Fortner, M.; Fox, H.; Fu, S.; Fuess, S.; Gadfort, T.; Galea, C. F.; Gallas, E.; Galyaev, E.; Garcia, C.; Garcia-Bellido, A.; Gavrilov, V.; Gay, P.; Geist, W.; Gelé, D.; Gerber, C. E.; Gershtein, Y.; Gillberg, D.; Ginther, G.; Gollub, N.; Gómez, B.; Goussiou, A.; Grannis, P. D.; Greenlee, H.; Greenwood, Z. D.; Gregores, E. M.; Grenier, G.; Gris, Ph.; Grivaz, J.-F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guo, J.; Guo, F.; Gutierrez, P.; Gutierrez, G.; Haas, A.; Hadley, N. J.; Haefner, P.; Hagopian, S.; Haley, J.; Hall, I.; Hall, R. E.; Han, L.; Hanagaki, K.; Hansson, P.; Harder, K.; Harel, A.; Harrington, R.; Hauptman, J. M.; Hauser, R.; Hays, J.; Hebbeker, T.; Hedin, D.; Hegeman, J. G.; Heinmiller, J. M.; Heinson, A. P.; Heintz, U.; Hensel, C.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hobbs, J. D.; Hoeneisen, B.; Hoeth, H.; Hohlfeld, M.; Hong, S. J.; Hooper, R.; Hossain, S.; Houben, P.; Hu, Y.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jain, S.; Jakobs, K.; Jarvis, C.; Jesik, R.; Johns, K.; Johnson, C.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Juste, A.; Käfer, D.; Kahn, S.; Kajfasz, E.; Kalinin, A. M.; Kalk, J. R.; Kalk, J. M.; Kappler, S.; Karmanov, D.; Kasper, J.; Kasper, P.; Katsanos, I.; Kau, D.; Kaur, R.; Kaushik, V.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. M.; Khatidze, D.; Kim, H.; Kim, T. J.; Kirby, M. H.; Kirsch, M.; Klima, B.; Kohli, J. M.; Konrath, J.-P.; Kopal, M.; Korablev, V. M.; Kothari, B.; Kozelov, A. V.; Krop, D.; Kryemadhi, A.; Kuhl, T.; Kumar, A.; Kunori, S.; Kupco, A.; Kurča, T.; Kvita, J.; Lacroix, F.; Lam, D.; Lammers, S.; Landsberg, G.; Lazoflores, J.; Lebrun, P.; Lee, W. M.; Leflat, A.; Lehner, F.; Lellouch, J.; Lesne, V.; Leveque, J.; Lewis, P.; Li, J.; Li, Q. Z.; Li, L.; Lietti, S. M.; Lima, J. G. R.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, Y.; Liu, Z.; Lobo, L.; Lobodenko, A.; Lokajicek, M.; Lounis, A.; Love, P.; Lubatti, H. J.; Lyon, A. L.; Maciel, A. K. A.; Mackin, D.; Madaras, R. J.; Mättig, P.; Magass, C.; Magerkurth, A.; Makovec, N.; Mal, P. K.; Malbouisson, H. B.; Malik, S.; Malyshev, V. L.; Mao, H. S.; Maravin, Y.; Martin, B.; McCarthy, R.; Melnitchouk, A.; Mendes, A.; Mendoza, L.; Mercadante, P. G.; Merkin, M.; Merritt, K. W.; Meyer, J.; Meyer, A.; Michaut, M.; Millet, T.; Mitrevski, J.; Molina, J.; Mommsen, R. K.; Mondal, N. K.; Moore, R. W.; Moulik, T.; Muanza, G. S.; Mulders, M.; Mulhearn, M.; Mundal, O.; Mundim, L.; Nagy, E.

    2007-10-01

    This Letter presents the first strong evidence for the resolution of the excited B mesons B1 and B2* as two separate states in fully reconstructed decays to B+(*)π-. The mass of B1 is measured to be 5720.6±2.4±1.4MeV/c2 and the mass difference ΔM between B2* and B1 is 26.2±3.1±0.9MeV/c2, giving the mass of the B2* as 5746.8±2.4±1.7MeV/c2. The production rate for B1 and B2* mesons is determined to be a fraction (13.9±1.9±3.2)% of the production rate of the B+ meson.

  16. 26 CFR 31.3306(b)-1 - Wages.

    Science.gov (United States)

    2010-04-01

    ... Unemployment Tax Act (Chapter 23, Internal Revenue Code of 1954) § 31.3306(b)-1 Wages. (a) Applicable law and... restaurant or hotel employees, or to seamen or other employees aboard vessels, since generally these items...

  17. ACTH Regulation of Adrenal SR-B1

    Directory of Open Access Journals (Sweden)

    Wen-Jun eShen

    2016-05-01

    Full Text Available The adrenal gland is one of the prominent sites for steroid hormone synthesis. Lipoprotein-derived cholesterol esters delivered via scavenger receptor, class B type 1 (SR-B1 constitute the dominant source of cholesterol for steroidogenesis, particularly in rodents. ACTH stimulates steroidogenesis through downstream actions on multiple components involved in steroidogenesis. Both acute and chronic ACTH treatment can modulate SR-B1 function including its transcription, its post transcriptional stability, its phosphorylation and dimerization status, as well as its interaction with other protein partners; all of which result in changes in the ability of SR-B1 to mediate HDL-cholesterol ester uptake and the supply of cholesterol for conversion to steroids. Here we provide a review of the recent findings on the regulation of adrenal SR-B1 function by ACTH.

  18. Supercritical Fluid Extraction of Aflatoxin B 1 from Soil

    Science.gov (United States)

    This research describes the development of a Supercritical Fluid Extraction (SFE) method to recover aflatoxin B1 from fortified soil. The effects of temperature, pressure, modifier (identity and percentage), and extraction type were assessed. Using the optimized SFE conditions, ...

  19. 76 FR 55651 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2011-09-08

    ... transmittal, policy justification, and Sensitivity of Technology. Dated: September 2, 2011. Aaron Siegel... Pursuant to Section 36(b)(1) of the Arms Export Control Act, as amended (i) Prospective Purchaser: Norway... be Sold: None. [[Page 55653

  20. On a generalization of B1( ) on C∗ -algebras

    Indian Academy of Sciences (India)

    Introduction. Let Ж be a complex and separable Hilbert space and let 多 (Ж ) be the set of bounded linear operators on Ж . Let be a open connected subset of complex plane C. A class of Cowen–Douglas operator with index one: B1( ) is defined as follows [6]: B1( ) =: {T ∈ 多 (Ж ): (i). ⊂ σ (T ) =: {λ ∈ C : T − λI is not invertible ...

  1. PROPAGATION-BASED CONSTRAINT SOLVER IN IMS

    Directory of Open Access Journals (Sweden)

    I.Ol. Blynov

    2012-03-01

    Full Text Available Article compiling the main ideas of creating propagation-based constraint solver, theoretical basis of constraint programming and its implementation in IMS (Insertion Modeling System

  2. Glaucoma and Cytochrome P4501B1 Gene Mutations

    Directory of Open Access Journals (Sweden)

    Mukesh Tanwar

    2010-01-01

    Full Text Available Developmental anomalies of the ocular anterior chamber angle may lead to an incomplete development of the structures that form the conventional aqueous outflow pathway. Thus, disorders that present with such dysfunction tend to be associated with glaucoma. Among them, Axenfeld-Rieger (ARS malformation is a rare clinical entity with an estimated prevalence of one in every 200,000 individuals. The changes in eye morphogenesis in ARS are highly penetrant and are associated with 50% risk of development of glaucoma. Mutations in the cytochrome P4501B1 (CYP1B1 gene have been reported to be associated with primary congenital glaucoma and other forms of glaucoma and mutations in pituitary homeobox 2 (PITX2 gene have been identified in ARS in various studies. This case was negative for PITX2 mutations and compound heterozygote for CYP1B1 mutations. Clinical manifestations of this patient include bilateral elevated intraocular pressure (>40 mmHg with increased corneal diameter (>14 mm and corneal opacity. Patient also had iridocorneal adhesions, anteriorly displaced Schwalbe line, anterior insertion of iris, broad nasal bridge and protruding umbilicus. This is the first study from north India reporting CYP1B1 mutations in Axenfeld-Rieger syndrome with bilateral buphthalmos and early onset glaucoma. Result of this study supports the role of CYP1B1 as a causative gene in ASD disorders and its role in oculogenesis.

  3. Digital Medien im Naturwissenschaftlichen Unterricht

    CERN Document Server

    Meßinger-Koppelt, Jenny

    2014-01-01

    „Morgens, 8 Uhr: Kurz vor der Schultür werden noch die neuesten Posts auf Facebook geliked, ein Selfie in die Whats-App-Gruppe hochgeladen oder auf YouTube noch ein #LOL-Video gestreamed. Dann klingelt es und die Digital Natives greifen wieder zu Stift, Papier und werden frontal mit Fakten gefüttert“ (A. Spang). Für Schüler sind Internet, Smartphone und Tablet-PC heute selbstverständlicher Teil ihrer Lernwelt. Und auch die Alltags- und Berufswelt ist ohne Informationstechnologien nicht mehr vorstellbar. Doch was bedeutet das für den Schulunterricht, insbesondere für die techniknahen Naturwissenschaften? Wie gelingt im Schulalltag die Balance zwischen Kreidezeit und interaktivem Whiteboard nach mittlerweile 30 Jahren Erfahrung mit Schulcomputern? Der vorliegende Sammelband präsentiert zahlreiche Praxisbeispiele aus dem Schulalltag zum sinnvollen Einsatz digitaler Medien im Biologie-, Chemie- und Physik-Unterricht sowie den aktuellen Stand der fachdidaktischen Forschung. Zu Wort kommen Hochschullehr...

  4. B1-Metallo-beta-Lactamases: Where do we stand?

    Science.gov (United States)

    Mojica, Maria F.; Bonomo, Robert A.; Fast, Walter

    2015-01-01

    Metallo-beta-Lactamases (MBLs) are class B β-lactamases that hydrolyze almost all clinically-available β-lactam antibiotics. MBLs feature the distinctive αβ/βα sandwich fold of the metallo-hydrolase / oxidoreductase superfamily and possess a shallow active-site groove containing one or two divalent zinc ions, flanked by flexible loops. According to sequence identity and zinc ion dependence, MBLs are classified into three subclasses (B1, B2 and B3), of which the B1 subclass enzymes have emerged as the most clinically significant. Differences among the active site architectures, the nature of zinc ligands, and the catalytic mechanisms have limited the development of a common inhibitor. In this review, we will describe the molecular epidemiology and structural studies of the most prominent representatives of class B1 MBLs (NDM-1, IMP-1 and VIM-2) and describe the implications for inhibitor design to counter this growing clinical threat. PMID:26424398

  5. RIA determination of aflatoxin B1 in food

    International Nuclear Information System (INIS)

    Bludovsky, R.

    1986-01-01

    The possibility was tested of the application of a commercial RIA kit in determining aflatoxin B 1 in foodstuffs. Stability, sensitivity, specificity and the effect of interfering materials were studied. Alternative methods were tested of sample preparation and a technique was proposed usable for food analysis. The kit was found to be mainly suitable for screening and for the determination of aflatoxin B 1 in concentrations exceedings 1 μg/kg. Lipids were found to cause a systematic positive error and should thus be removed by extraction prior to analysis. (author). 10 tabs., 15 refs

  6. Analysis of IMS spectra using neural networks

    Energy Technology Data Exchange (ETDEWEB)

    Bell, S.E.

    1992-01-01

    Ion mobility spectrometry (IMS) has been used for over 20 years, and IMS coupled to gas chromatography (GC/IMS) has been used for over 10 years. There still is no systematic approach to IMS spectral interpretation such as exists for mass spectrometry and infrared spectrometry. Neural networks, a form of adaptive pattern recognition, were examined as a method of data reduction for IMS and GC/IMS. A wide variety of volatile organics were analyzed using IMS and GC/IMS and submitted to different networks for identification. Several different networks and data preprocessing algorithms were studied. A network was linked to a simple rule-based expert system and analyzed. The expert system was used to filter out false positive identifications made by the network using retention indices. The various network configurations were compared to other pattern recognition techniques, including human experts. The network performance was comparable to human experts, but responded much faster. Preliminary comparison of the network to other pattern recognition showed comparable performance. Linkage of the network output to the rule-based retention index system yielded the best performance.

  7. Analysis of IMS spectra using neural networks

    Energy Technology Data Exchange (ETDEWEB)

    Bell, S.E.

    1992-09-01

    Ion mobility spectrometry (IMS) has been used for over 20 years, and IMS coupled to gas chromatography (GC/IMS) has been used for over 10 years. There still is no systematic approach to IMS spectral interpretation such as exists for mass spectrometry and infrared spectrometry. Neural networks, a form of adaptive pattern recognition, were examined as a method of data reduction for IMS and GC/IMS. A wide variety of volatile organics were analyzed using IMS and GC/IMS and submitted to different networks for identification. Several different networks and data preprocessing algorithms were studied. A network was linked to a simple rule-based expert system and analyzed. The expert system was used to filter out false positive identifications made by the network using retention indices. The various network configurations were compared to other pattern recognition techniques, including human experts. The network performance was comparable to human experts, but responded much faster. Preliminary comparison of the network to other pattern recognition showed comparable performance. Linkage of the network output to the rule-based retention index system yielded the best performance.

  8. IMS Learning Design Frequently Asked Questions

    NARCIS (Netherlands)

    Tattersall, Colin; Manderveld, Jocelyn; Hummel, Hans; Sloep, Peter; Koper, Rob; De Vries, Fred

    2004-01-01

    This list of frequently asked questions was composed on the basis of questions asked of the Educational Technology Expertise Centrum. The questions addessed are: Where can I find the IMS Learning Design Specification? What is meant by the phrase “Learning Design”? What is the IMS LD Specification

  9. GEOSPATIAL IT/IM QA CHECKLIST

    Science.gov (United States)

    Quality assurance (QA) of information technology (IT) and Information Management (IM) systems help to ensure that the end product is of known quality and integrity. As the complexity of IT & IM processes increase, so does the need for regular QA evaluation. The areas revi...

  10. On a generalization of B1( ) on C∗ -algebras

    Indian Academy of Sciences (India)

    Proc. Indian Acad. Sci. (Math. Sci.) Vol. 124, No. 2, May 2014, pp. 243–253. c Indian Academy of Sciences. On a generalization of B1( ) on C∗. -algebras. KUI JI. Department of Mathematics, Hebei Normal University, Shijiazhuang, ...... [4] Clark D N and Misra G, The curvature function and similarity of operators, International.

  11. Inhibiting Aspergillus flavus growth and degrading aflatoxin B1 by ...

    African Journals Online (AJOL)

    Aflatoxin B1 (AFB1) is a type of toxin produced by Aspergillus flavus, which has a negative effect on animal production and economic profits. In order to inhibit A. flavus growth and degrade aflatoxin, the optimal proportion of beneficial microbes such as Lactobacillus casei, Bacillus subtilis and Pichia anomala were selected.

  12. 26 CFR 31.3231(b)-1 - Who are employees.

    Science.gov (United States)

    2010-04-01

    ... TAXES AND COLLECTION OF INCOME TAX AT SOURCE EMPLOYMENT TAXES AND COLLECTION OF INCOME TAX AT SOURCE Railroad Retirement Tax Act (Chapter 22, Internal Revenue Code of 1954) General Provisions § 31.3231(b)-1... operations consisting of the mining of coal, the preparation of coal, the handling (other than movement by...

  13. Aflatoxin B 1 producing potential of Aspergillus flavus strains ...

    African Journals Online (AJOL)

    Aflatoxin B 1 (AFB1) producing potential of different strains of Aspergillus flavus, isolated from 1,200 stored rice grains collected from 43 locations in 20 rice growing states in India was investigated. Eighty-five strains of A. flavus were isolated from the discolored rice grains and tested for their AFB1 producing potential on ...

  14. 26 CFR 1.167(b)-1 - Straight line method.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Straight line method. 1.167(b)-1 Section 1.167(b... Straight line method. (a) In general. Under the straight line method the cost or other basis of the... may be reduced to a percentage or fraction. The straight line method may be used in determining a...

  15. 26 CFR 1.411(b)-1 - Accrued benefit requirements.

    Science.gov (United States)

    2010-04-01

    ... TAX (CONTINUED) INCOME TAXES Pension, Profit-Sharing, Stock Bonus Plans, Etc. § 1.411(b)-1 Accrued... participant in the J Corporation's plan on January 1, 1980. As of December 31, 1990, B's compensation history... pension benefit plans, for time participation deemed to begin. (2) Special rules—(i) A plan shall not be...

  16. 26 CFR 1.643(b)-1 - Definition of income.

    Science.gov (United States)

    2010-04-01

    ... (CONTINUED) INCOME TAXES Estates, Trusts, and Beneficiaries § 1.643(b)-1 Definition of income. For purposes... amount of income of an estate or trust for the taxable year determined under the terms of the governing... adjustments between income and principal to fulfill the trustee's duty of impartiality between the income and...

  17. APRIL promotes B-1 cell-associated neoplasm

    NARCIS (Netherlands)

    Planelles, Lourdes; Carvalho-Pinto, Carla E.; Hardenberg, Gijs; Smaniotto, Salette; Savino, Wilson; Gómez-Caro, Ruth; Alvarez-Mon, Melchor; de Jong, Joan; Eldering, Eric; Martínez-A, Carlos; Medema, Jan Paul; Hahne, Michael

    2004-01-01

    A tumor-supporting role for the TNF-like ligand APRIL has been suggested. Here we describe that 9- to 12-month-old APRIL transgenic mice develop lymphoid tumors that originate from expansion of the peritoneal B-1 B cell population. Aging APRIL transgenic mice develop progressive hyperplasia in

  18. 76 FR 72682 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2011-11-25

    ... aircraft Australia already received or is under contract to purchase. The proposed sale of this equipment... Pursuant to Section 36(b)(1) of the Arms Export Control Act, as amended (i) Prospective Purchaser: Australia (ii) Total Estimated Value: Major Defense Equipment $ 225 million. Other $ 75 million. TOTAL $ 300...

  19. Osmotic stress upregulates the transcription of thiamine (vitamin B1 ...

    African Journals Online (AJOL)

    Syamimi Mimi

    2016-07-20

    Jul 20, 2016 ... Thiamine or vitamin B1 comprises a pyrimidine moiety and a thiazole moiety. Thiamine pyrophosphate. (TPP), the active form of thiamine, acts as a cofactor for various major enzymes, for example, transketolase (TK), α-ketoglutarate dehydrogenase (KGDH) and pyruvate dehydrogenase (PDH). It is.

  20. Isolating Barley (Hordeum vulgare L.) B1 Hordein Gene Promoter ...

    African Journals Online (AJOL)

    Yomi

    2012-04-10

    Apr 10, 2012 ... Isolating Barley (Hordeum vulgare L.) B1 Hordein Gene. Promoter and Using Sequencing Analaysis. For The Identification of Conserved Regulatory. Elements By Bioinformatic Tools. Kobra Nalbandi1, Bahram Baghban Kohnehrouz2*, Khalil Alami Saeed1 and Ashraf. Gholizadeh3. 1Ramin Agricultural ...

  1. 76 FR 68432 - 36(b)(1) Arms Sales Notification

    Science.gov (United States)

    2011-11-04

    ... participant in the Organization for Security and Cooperation in Europe (OSCE) and in early 1995 assumed the co... of the Secretary 36(b)(1) Arms Sales Notification AGENCY: Defense Security Cooperation Agency... contribute to the foreign policy and national security of the United States by improving the security of a...

  2. Studies on image quality, high contrast resolution and dose for the axial skeleton and limbs with a new, dedicated CT system (ISO-C-3D); Untersuchungen zur Bildqualitaet, Hochkontrastaufloesung und Dosis am Stamm- und Gliedmassenskelett mit einem neuen dedizierten CT-System (ISO-C-3D)

    Energy Technology Data Exchange (ETDEWEB)

    Rock, C.; Kotsianos, D.; Linsenmaier, U. [Klinikum der Universitaet Muenchen, Muenchen (Germany). Inst. fuer Klinische Radiologie; Fischer, T. [Klinikum der Universitaet Muenchen, Muenchen (DE). Inst. fuer Klinische Radiologie] (and others)

    2002-02-01

    verwertbare Bildqualitaet; Score 4: hervorragende Bildqualitaet). Zusaetzlich erfolgten Dosismessungen und Hochkontraststudien an einem adaptierten Hochkontrastphantom (Catphan, Phantom Laboratories) im Vergleich zur Niedrigdosis- und Standarddosis-CT. Ergebinsse: Die Untersuchungen kleinerer Gelenke wie Hand-, Sprung-, Ellbogen- und Kniegelenk lieferten eine diagnostische Bildqualitaet (Scoremittelwerte 3-4) bei einer applizierten Dosis im Bereich der einer Niedrigdosis-CT. Dagegen war die Bildqualitaet der Hueftgelenks- und Wirbelsaeulenuntersuchungen trotz Dosiserhoehung um 85% eingeschraenkt (Scorewerte 2-3) und die der Schultergelenksuntersuchungen von schlechter Qualitaet (Score 1). Die Bilddaten der Hochkontraststudien zeigten in der xy-Achse eine der CT vergleichbare Hochkontrastaufloesung (9 LP/cm). Schlussfolgerung: Die vorlaeufigen Ergebnisse zeigen, dass die Bildqualitaet der ISO-C-3D-Untersuchungen von peripheren Gelenken von diagnostischer Qualitaet ist. Am Hueftgelenk und am Stammskelett ist die Bildqualitaet nicht adaequat und erscheint bisher nicht ausreichend zur Frakturdiagnostik. (orig.)

  3. Ion Mobility Spectrometry (IMS) and Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Shvartsburg, Alexandre A.

    2010-04-20

    In a media of finite viscosity, the Coulomb force of external electric field moves ions with some terminal speed. This dynamics is controlled by “mobility” - a property of the interaction potential between ions and media molecules. This fact has been used to separate and characterize gas-phase ions in various modes of ion mobility spectrometry (IMS) developed since 1970. Commercial IMS devices were introduced in 1980-s for field detection of volatile traces such as explosives and chemical warfare agents. Coupling to soft-ionization sources, mass spectrometry (MS), and chromatographic methods in 1990-s had allowed IMS to handle complex samples, enabling new applications in biological and environmental analyses, nanoscience, and other areas. Since 2003, the introduction of commercial systems by major instrument vendors started bringing the IMS/MS capability to broad user community. The other major development of last decade has been the differential IMS or “field asymmetric waveform IMS” (FAIMS) that employs asymmetric time-dependent electric field to sort ions not by mobility itself, but by the difference between its values in strong and weak electric fields. Coupling of FAIMS to conventional IMS and stacking of conventional IMS stages have enabled two-dimensional separations that dramatically expand the power of ion mobility methods.

  4. Instant Messaging (IM used in the Workplace

    Directory of Open Access Journals (Sweden)

    Norizah Aripin

    2011-09-01

    Full Text Available Developments in information communication technologies (ICT is growing rapidly and organizations use the ICT for business tasks. Group organization consists of various levels of management receive technology development to ensure the management and administration to be more effective and efficient. Organizations now have the equipment of modern technology which allows the task is done by using electronic devices to ensure increased productivity among lower, higher and professional management. Computer mediated communication, such as Instant Messaging (IM used in the workplace other than the use of e-mail. This study discusses the frequency and patterns of use of IM in the workplace. Quantitative methods of survey questionnaires and qualitative methods of conversation analysis used in this study. For survey questionnaire, 379 organizations status MSC involved as respondents. Meanwhile, the conversation analysis approach, using five examples of IM conversations from five different organizations for sample analysis. Data was then analyzed using descriptive (survey questionnaire and thematic analysis (analysis of IM conversations to identify the specific themes on IM usage motive at work. The study found that 279 respondents have used IM in organization. Motives intended use of IM to provide work orders, obtain information, maintain relationship together and express feelings.

  5. CobB1 deacetylase activity in Streptomyces coelicolor

    Czech Academy of Sciences Publication Activity Database

    Mikulík, Karel; Felsberg, Jürgen; Kudrnáčová, E.; Bezoušková, Silvia; Šetinová, Dita; Stodůlková, Eva; Zídková, J.; Zídek, Václav

    2012-01-01

    Roč. 90, č. 2 (2012), s. 179-187 ISSN 0829-8211 R&D Projects: GA AV ČR(CZ) IAA500110805; GA ČR GA303/09/0475 Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z50200510 Keywords : sirtuin * NAD(+)dependent deacetylation activity CobB1 * Streptomyces coeliocolor Subject RIV: EE - Microbiology, Virology Impact factor: 2.915, year: 2012

  6. 118-B-1 burial ground excavation treatability test report

    International Nuclear Information System (INIS)

    1995-08-01

    This treatability investigation focused on the feasibility of excavating, analytical screening, and handling waste materials from the 118-B-1 Burial Ground located in the 100 B/C Area of the Hanford Site. The 118-B-1 Burial Ground consists of approximately 24 trenches on a 7-acre parcel. Solid low-level radioactive wastes and other debris and trash associated with reactor operations were disposed in 28 burial grounds in the 100 Area between 1944 and 1973. The majority of waste generated from routine reactor operations was placed in seven primary burial grounds, including 118-B-1. The 118-B-1 Burial Ground was selected as the location to perform this treatability test based on the availability of historical data for this site, and because it was thought to be representative of other primary-use burial grounds in the 100 Area. Geophysical surveys were conducted over the burial ground to map the concentrations of waste and aid in the selection of test pit excavation locations. The test plan developed for this study integrated the Streamlined Approach for Environmental Restoration (SAFER), a US Department of Energy (DOE) initiative based on both the Data Quality Objective (DQO) process and the observational approach. This treatability test is the first one at the Hanford Site to use the SAFER approach. The purpose of this study was (1) to support development of the Proposed Plan and Record of Decision, which would identify the approach to be used for burial ground remediation and (2) to provide specific engineering information for receiving waste generated from the 100 Area removal actions. The results of the treatability test can be used to determine the feasibility of performing excavation, analytical screening, and handling of burial ground materials from similar burial grounds

  7. Biochemical characterization of the GM2 gangliosidosis B1 variant

    Directory of Open Access Journals (Sweden)

    Tutor J.C.

    2004-01-01

    Full Text Available The deficiency of the A isoenzyme of ß-hexosaminidase (Hex produced by different mutations of the gene that codes for the alpha subunit (Tay-Sachs disease has two variants with enzymological differences: the B variant consists of the absence of Hex A isoenzyme and the B1 variant produces an inactive Hex A isoenzyme for the hydrolysis of the GM2 ganglioside and synthetic substrates with negative charge. In contrast to the early childhood form of the B variant, the B1 variant appears at a later clinical stage (3 to 7 years of age with neurodegenerative symptoms leading to the death of the patient in the second decade of life. The most frequent mutation responsible for the GM2 gangliosidosis B1 variant is R178H, which has a widespread geographic and ethnic distribution. The highest incidence has been described in Portugal, which has been suggested as the point of origin of this mutation. Biochemical characterization of this lysosomal disease is carried out using negatively charged synthetic alpha subunit-specific sulfated substrates, since Hex A isoenzyme heat-inactivation assays are not applicable. However, the determination of the apparent activation energy of Hex using the neutral substrate 3,3'-dichlorophenolsulfonphthaleinyl N-acetyl-ß-D-glucosaminide, may offer a valid alternative. The presence of an alpha subunit in the alphaß heterodimer Hex A means that its activation energy (41.8 kJ/mol is significantly lower than that of the ßß homodimer Hex B (75.1 kJ/mol; however, as mutation inactivates the alpha subunit, the Hex A of the B1 variant presents an activation energy that is similar to that of the Hex B isoenzyme.

  8. Abnormal Expressions of Age, RAGE, TGF- b1 and TGF- b1 Receptor in Colonic Wall Contributed to STZ-Induced Diabetic Colon Remodeling

    DEFF Research Database (Denmark)

    Zhao, Jingbo; Gregersen, Hans

    2016-01-01

    glycation end product (AGE) and AGE receptor (RAGE) were up-regulated in the diabetic colon wall (2). However, it lacks data in relation to the association between AGE, RAGE, transforming growth factor- b1 (TGF-b1) and TGFb1 receptor expressions with colon morphological and biomechanical remodeling...... glucose level was measured. The parameters of morphometric and biomechanical properties of colonic segments were obtained from diabetic (DM) and normal (Con) rats. The expressions of AGE, RAGE, TGF- b1 and TGF- b1 receptor were detected in different layers of the colon by immunohistochemistry. In order...... to determine the expressions of AGE, RAGE, TGF- b1 and TGF- b1 receptor in association with other parameters, and to see interrelation among AGE, RAGE, TGF- b1 and TGF- b1 receptor expressions, the multiple linear regression analysis was done. Results: The expressions of AGE, RAGE, TGF-b1 and TGF- b1 receptor...

  9. SOFIA FIFI-LS Observations of Sgr B1

    Science.gov (United States)

    Simpson, Janet P.; Colgan, Sean; Cotera, Angela; Kaufman, Michael; Stolovy, Susan

    2018-01-01

    Although Sgr B1 is the third most luminous H II region in the Galactic Center, after the region ionized by the central star cluster Sgr A and the very young star-forming region Sgr B2, it has surprisingly not been well studied. Because Sgr B1 is found with Sgr B2 in a common envelope of molecular gas and far-infrared (FIR) emission, it has been assumed that the two sources are physically related, even though the ionized gas of Sgr B1 is much more extended and there are evolved-star indicators of a significantly greater age than Sgr B2. This presents a problem because the current paradigm of Galactic Center gas motions and star formation requires that the entire Sgr B complex must have reasonably similar ages. To clarify the situation, we have mapped Sgr B1 with the FIFI-LS spectrometer on SOFIA in the FIR lines of [O III] 52 and 88 micron, [O I] 146 micron, and [C II] 158 micron. For the most part, we find little correlation of the density-sensitive ratio of the [O III] lines with the radio VLA images or the FIR Herschel PACS images, from which we infer that the structures seen in those images are not due to density enhancements but are due to bubbles of gas and dust seen edge-on. The [C II] 158 micron line correlates much better with the structure seen in the Herschal PACS Blue 60-85 micron image than it does with the Red 130-210 micron image, which is dominated by the massive molecular cloud seen at higher Galactic latitude. Sgr B1 is largely covered by spectra from the Spitzer Infrared Spectrograph Archive, from which we measure mid-infrared lines of [Ne II], [Ne III], [Si II], [S III], and [Ar II]. From the ratios of these lines with the observed FIR lines we find that there are at least six small regions with significantly higher excitation than their surroundings; we infer that these regions contain the stars that excite the gas. Our presentation will describe these results in further detail.

  10. Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections.

    Science.gov (United States)

    Gaye, M M; Kurulugama, R; Clemmer, D E

    2015-10-21

    Ion mobility spectrometry techniques (IMS and IMS-IMS) combined with collision-induced dissociation (CID) and mass spectrometry (MS) are used to investigate the structures of singly-lithiated carbohydrate isomers. With the exception of some favorable cases, IMS-MS analyses of underivatized carbohydrates reveal that most isobaric precursor ions have similar collision cross sections (ccs). In contrast, ccs values for isomeric fragment ions obtained by IMS-CID-IMS-MS analysis are often different, and thus appear to be useful as a means of distinguishing the isomeric precursors. We report values of ccs (in He) for precursor- and associated-fragment ions for three monosaccharide isomers (glucose, galactose and fructose), ten disaccharide isomers (sucrose, leucrose, palatinose, trehalose, cellobiose, β-gentiobiose, isomaltose, maltose, lactose and melibiose), and three trisaccharide isomers (raffinose, melezitose and maltotriose). These values are discussed as a means of differentiating precursor carbohydrates.

  11. Effect of an esterified glucomannan on laying hens exposed to combined mycotoxins (aflatoxin B1, zearalenone and fumonisin B1

    Directory of Open Access Journals (Sweden)

    A. Zaghini

    2011-03-01

    Full Text Available Mycotoxin toxicity depends on species, exposure time, age, sex, health and possible synergistic effects of other mycotoxins present in feed. In poultry, aflatoxin B1 (AFB1 and fumonisin B1 (FB1 are associated with poor growth performance, lowered feed utilization efficiency, liver damage and immunosuppression; metabolites may persist in tissues and eggs. Exposure of mature hens to zearalenone (ZEN apparently does not cause adverse effects, but ZEN residues and α and β zearalenol persist in liver and muscle (Kuiper-Goodman et al., 1987 and may be transmitted to egg yolk (Dailey et al., 1990. Various treatments and dietary strategies have been tried to reduce mycotoxin levels in contaminated commodities...

  12. Innovationsmanagement im Marketing: Anleitung zur Innovations-Sensibilisierung und deren Umsetzung im Rahmen der Neuen Lernkultur

    OpenAIRE

    Mangisch, Christian; Blatter, Martin

    2009-01-01

    Der Kurs “Innovationsmanagement im Marketing“ vermittelt den Teilnehmenden Wissen zum Innovationsmanagement, zum innovativen Team und zu innovativen Marketing Trends. Diese Kurse sind online auf dem folgenden Link www.ritzycampus.ch zu finden. Die Teilnehmer müssen für den Online-Kurs keine speziellen Kenntnisse oder Voraussetzungen mitbringen. Diese Online-Kurse im Fach Marketing sind an ein breites Publikum gerichtet. Sie ermöglichen den Studierenden im Eigenstudium die Grundlagen der Theme...

  13. I'm a Map, I'm a Green Tree

    Science.gov (United States)

    Anderson, Daniel

    2010-01-01

    I'm talking about the ways we represent ourselves and our world. I've put some thoughts on the topic together here--a gathering that enacts new media creating and takes up conceptual layers like metaphors, models, and composing. The primary sources are videos from the Get a Mac campaign, aka I'm a Mac; I'm a PC ads. Posthuman concepts blending…

  14. Interkulturelle Begegnungen im kontextfernen Sprachunterricht

    Directory of Open Access Journals (Sweden)

    Tatjana Louis

    2015-04-01

    Full Text Available Dieser Beitrag stellt eine Aktionsforschungs-Untersuchung vor, die zwischen 2010 und 2012 an der Universidad de los Andes in Bogotá durchgeführt wurde. Ausgangspunkt war die These, dass die geographische Entfernung Kolumbiens zu Deutschland und die damit einhergehenden fehlenden Möglichkeiten der Lernenden, ihr im Unterricht erworbenes Wissen zu kontextualisieren, sich erschwerend auf die Entwicklung interkultureller Kompetenzen auswirkt. Mit dem Ziel, diese Schwierigkeit zu kompensieren, wurden zunächst die spezifischen Bedürfnisse der Lernenden ermittelt und verschiedene Lehrbücher hinsichtlich ihrer Angebote auf diese Bedürfnisse evaluiert. Schließlich wird gezeigt, wie die Ergebnisse in die Unterrichtspraxis implementiert wurden.   This article present s the results of an Action Research study carried out between 2010 and 2012 at the Colombian Universidad de los Andes. Its starting point was the hypothesis that the geographical distance between Colombia and Germany makes it more difficult for the students to contextualize the knowledge acquired in class, therefore impeding the development of intercultural competences. In order to compensate for this lack of contextualization the investigators first established the students´ specific needs. Then they evaluated different textbooks with regard to what they are offering to respond to those needs. Finally, the article describes how the findings were implemented in the classroom.

  15. Literatur zu Gast im Globe

    CERN Multimedia

    2006-01-01

    Der deutsche Schriftsteller Thomas LEHR liest am 23. März 2006 aus seinem Roman '42', einem philosophischen Abenteuerroman über die Suche nach der Zeit, erzählt in einer funkelnden und souveränen Sprache. Eine Welt von vernichtender Schönheit und bizarren Schrecken wartet auf eine Besuchergruppe, die in der Nähe von Genf die unterirdischen Anlagen des Forschungszentrums für Teilchenphysik CERN besichtigt. Als sie um 12:47:42 wieder ans Tageslicht tritt, scheint ganz Europa in einen Dornröschenschlaf gefallen. Allein die 70 Besucher können sich noch bewegen. Monate und Jahre verharrt die Welt wie in einer riesigen dreidimensionalen Fotografie, bis ein wiederum schockierendes Ereignis die 'Chronifizierten' aus ihren physikalischen Spekulationen und seltsamen Lebensformen reisst. Zu dieser literarischen Veranstaltung im 'Globe of Science and Innovation' laden gemeinsam das Generalkonsulat der Bundesrepublik Deutschland in Genf und CERN ein. Vor Beginn der Lesung wird eine Führung in deutscher Sprac...

  16. [Variation im heutigen Deutsch...] / Laura Tidrike

    Index Scriptorium Estoniae

    Tidrike, Laura

    2008-01-01

    Arvustus: Variation im heutigen Deutsch : Perspektiven für den Sprachunterricht / hrsg. v. Eva Neuland. Frankfurt am Main : Lang, 2006. (Sprache - Kommunikation - Kultur. Soziolinguistische Beiträge ; Vol. 4)

  17. IMS Mitigation Target Areas - 2010 [ds673

    Data.gov (United States)

    California Department of Resources — Mitigation Target Areas (MTA) were developed by the California Department of Fish and Game for the Interim Mitigation Strategy (IMS). The MTAs are an identification...

  18. Verletzungen und Fehlbeanspruchungen im leistungsorientierten Rudersport

    OpenAIRE

    Bussian, Marc Robert

    2004-01-01

    Die Stellung der Breitensportart Rudern als gesundheitsfördernde Sportform ist in der Literatur gleichlautend positiv beschrieben. Im leistungsorientierten Rudersport müssen neben den Verletzungen und Fehlbeanspruchungen der eigentlichen Sportart die unabdingbaren Nebentrainingsformen berücksichtigt werden. In den neunziger Jahren vollzog sich ein trainingsmethodischer Wandel, die Einführung eines erschwinglichen Rudersimulators und eine technische Weiterentwicklung im Boots- und Ruderbau. Ei...

  19. IMS-MS and IMS-IMS investigation of the structure and stability of dimethylamine-sulfuric acid nanoclusters.

    Science.gov (United States)

    Ouyang, Hui; He, Siqin; Larriba-Andaluz, Carlos; Hogan, Christopher J

    2015-03-12

    Recent studies of new particle formation events in the atmosphere suggest that nanoclusters (i.e, the species formed during the early stages of particle growth which are composed of 10(1)-10(3) molecules) may consist of amines and sulfuric acid. The physicochemical properties of sub-10 nm amine-sulfuric acid clusters are hence of interest. In this work, we measure the density, thermostability, and extent of water uptake of <8.5 nm effective diameter dimethylamine-sulfuric (DMAS) nanoclusters in the gas phase, produced via positive electrospray ionization. Specifically, we employ three systems to investigate DMAS properties: ion mobility spectrometry (IMS, with a parallel-plate differential mobility analyzer) is coupled with mass spectrometry to measure masses and collision cross sections for <100 kDa positively charged nanoclusters, two differential mobility analyzers in series (IMS-IMS) are used to examine thermostability, and finally a differential mobility analyzer coupled to an atmospheric pressure drift tube ion mobility spectrometer (also IMS-IMS) is used for water uptake measurements. IMS-MS measurements reveal that dry DMAS nanoclusters have densities of ∼1567 kg/m(3) near 300 K, independent of the ratio of dimethylamine to sulfuric acid originally present in the electrospray solution. IMS-IMS thermostability studies reveal that partial pressures of DMAS nanoclusters are dependent upon the electrospray solution concentration ratio, R = [H2SO4]/[(CH3)2NH]. Extrapolating measurements, we estimate that dry DMAS nanoclusters have surface vapor pressures of order 10(-4) Pa near 300 K, with the surface vapor pressure increasing with increasing values of R through most of the probed concentration range. This suggests that nanocluster surface vapor pressures are substantially enhanced by capillarity effects (the Kelvin effect). Meanwhile, IMS-IMS water uptake measurements show clearly that DMAS nanoclusters uptake water at relative humidities beyond 10% near 300

  20. A search for cyanopolyynes in L1157-B1

    Science.gov (United States)

    Mendoza, Edgar; Lefloch, B.; Ceccarelli, C.; Kahane, C.; Jaber, A. A.; Podio, L.; Benedettini, M.; Codella, C.; Viti, S.; Jimenez-Serra, I.; Lepine, J. R. D.; Boechat-Roberty, H. M.; Bachiller, R.

    2018-04-01

    We present here a systematic search for cyanopolyynes in the shock region L1157-B1 and its associated protostar L1157-mm in the framework of the Large Program`Astrochemical Surveys At IRAM' (ASAI), dedicated to chemical surveys of solar-type star-forming regions with the IRAM 30-m telescope. Observations of the millimeter windows between 72 and 272 GHz permitted the detection of HC3N and its 13C isotopologues, and HC5N (for the first time in a protostellar shock region). In the shock, the analysis of the line profiles shows that the emission arises from the outflow cavities associated with L1157-B1 and L1157-B2. Molecular abundances and excitation conditions were obtained from the analysis of the Spectral Line Energy Distributions under the assumption of Local Thermodynamical Equilibrium or using a radiative transfer code in the Large Velocity Gradient approximation. Towards L1157 mm, the HC3N emission arises from the cold envelope (T_rot=10K) and a higher-excitation region (Trot = 31K) of smaller extent around the protostar. We did not find any evidence of 13C or D fractionation enrichment towards L1157-B1. We obtain a relative abundance ratio HC3N/HC5N of 3.3 in the shocked gas. We find an increase by a factor of 30 of the HC3N abundance between the envelope of L1157-mm and the shock region itself. Altogether, these results are consistent with a scenario in which the bulk of HC3N was produced by means of gas phase reactions in the passage of the shock. This scenario is supported by the predictions of a parametric shock code coupled with the chemical model UCL_CHEM.

  1. Rapid Immunoenzyme Assay of Aflatoxin B1 Using Magnetic Nanoparticles

    Directory of Open Access Journals (Sweden)

    Alexandr E. Urusov

    2014-11-01

    Full Text Available The main limitations of microplate-based enzyme immunoassays are the prolonged incubations necessary to facilitate heterogeneous interactions, the complex matrix and poorly soluble antigens, and the significant sample dilutions often required because of the presence of organic extractants. This study presents the use of antibody immobilization on the surface of magnetic particles to overcome these limitations in the detection of the mycotoxin, aflatoxin B1. Features of the proposed system are a high degree of nanoparticle dispersion and methodologically simple immobilization of the antibodies by adsorption. Reactions between the immobilized antibodies with native and labeled antigens are conducted in solution, thereby reducing the interaction period to 5 min without impairing the analytical outcome. Adsorption of immunoglobulins on the surface of magnetic nanoparticles increases their stability in aqueous-organic media, thus minimizing the degree of sample dilution required. Testing barley and maize extracts demonstrated a limit of aflatoxin B1 detection equal to 20 pg/mL and total assay duration of 20 min. Using this method, only the 3-fold dilution of the initial methanol/water (60/40 extraction mixture in the microplate wells is necessary. The proposed pseudo-homogeneous approach could be applied toward immunodetection of a wide range of compounds.

  2. Synthesis of Polyclonal Antibodies against Aflatoxin B1

    Directory of Open Access Journals (Sweden)

    Wiyogo Prio Wicaksono

    2015-09-01

    Full Text Available Polyclonal antibodies of aflatoxin B1 were successfully produced from New Zealand White female rabbits after immunization by the hapten of aflatoxin B1-carboxymethyl hydroxylamine hemihydrochloride (AFB1-CMO conjugated with bovine serum albumin (BSA as the antigen. The hapten was synthesized using the carbodiimide method with CMO as a linker. Absorption peaks at 362, 264, and 218 nm were observed as a result of characterization with UV-Vis spectroscopy, while IR spectroscopy showed peaks at 3448 cm-1 and 1642 cm-1 attributable to the hydroxyl and nitrile groups, respectively. Furthermore, mass spectrometry showed fragmentation at the m/z of 386, 368.2, and 310, which confirms that the hapten of AFB1-CMO was successfully synthesized. The hapten was then conjugated with BSA to serve as an antigen of AFB1 when it was injected into the rabbits. The specificity of the antigen towards its antibody and the confirmation of hapten-BSA conjugation were characterized using the dot blot immunoassay, which showed a BSA concentration of 1.74 mg/mL. Two weeks after the primary immunization by its antigen, agar gel precipitation testing showed that the rabbit blood serum had positive results for polyclonal antibodiest against AFB1 with the highest concentration of antibodiest of 2.19 mg/mL.

  3. DELF B1 200 activités

    CERN Document Server

    Bloomfield, Anatole

    2006-01-01

    L'ouvrage est organisé par compétences de réception et de production. Chaque partie propose d'abord une présentation de l'épreuve correspondante, puis les activités de préparation suivies d'épreuves-types et d'une auto-évaluation. Dans le cadre d'une approche actionnelle, les activités fournissent les outils nécessaires à la préparation des examens. Elles donnent des exemples concrets répondant aux attentes des candidats comme à celle des examinateurs. LeNouveau DELF B1 propose des activités précises en vue d'atteindre les compétences nécessaires aux épreuves (oral, écrit, réception, production); sa simplicité et sa pédagogie en font une méthode complète. Dans la tradition de la collection Nouvel Entraînez-vous, cet ouvrage est pratique, didactique et se prête à une utilisation en autonomie ou en classe.Le niveau B1 dit " avancé " évalue un niveau de compétence où l'apprenant peut se considérer comme étant indépendant. Arrivé à ce stade, il est apte de comprendre l'inform...

  4. Integrating IMS Learning Design and IMS Question and Test Interoperability using CopperCore Service Integration

    NARCIS (Netherlands)

    Vogten, Hubert; Martens, Harrie; Nadolski, Rob; Tattersall, Colin; Van Rosmalen, Peter; Koper, Rob

    2006-01-01

    Please, cite this publication as: Vogten, H., Martens, H., Nadolski, R., Tattersall, C., van Rosmalen, P., & Koper, R. (2006). Integrating IMS Learning Design and IMS Question and Test Interoperability using CopperCore Service Integration. Proceedings of International Workshop in Learning Networks

  5. Integrating IMS LD and IMS QTIv2 using CopperCore Service Integration

    NARCIS (Netherlands)

    Vogten, Hubert

    2006-01-01

    Vogten, H. (2006). Integrating IMS LD and IMS QTIv2 using CopperCore Service Integration. Presentation at International Workshop in Learning Networks for Lifelong Competence Development. March, 30-31, 2006. Sofia, Bulgaria: TENCompetence Conference. Retrieved June 30th, 2006, from

  6. CopperCore Service Integration, Integrating IMS Learning Design and IMS Question and Test Interoperability

    NARCIS (Netherlands)

    Vogten, Hubert; Martens, Harrie; Nadolski, Rob; Tattersall, Colin; Van Rosmalen, Peter; Koper, Rob

    2006-01-01

    Vogten, H., Martens, H., Nadolski, R., Tattersall, C., Rosmalen, van, P., Koper, R., (2006). CopperCore Service Integration, Integrating IMS Learning Design and IMS Question and Test Interoperability. Proceedings of the 6th IEEE International Conference on Advanced Learning Technologies (pp.

  7. Asymmetric Dual-Band Tracking Technique for Optimal Joint Processing of BDS B1I and B1C Signals.

    Science.gov (United States)

    Wang, Chuhan; Cui, Xiaowei; Ma, Tianyi; Zhao, Sihao; Lu, Mingquan

    2017-10-16

    Along with the rapid development of the Global Navigation Satellite System (GNSS), satellite navigation signals have become more diversified, complex, and agile in adapting to increasing market demands. Various techniques have been developed for processing multiple navigation signals to achieve better performance in terms of accuracy, sensitivity, and robustness. This paper focuses on a technique for processing two signals with separate but adjacent center frequencies, such as B1I and B1C signals in the BeiDou global system. The two signals may differ in modulation scheme, power, and initial phase relation and can be processed independently by user receivers; however, the propagation delays of the two signals from a satellite are nearly identical as they are modulated on adjacent frequencies, share the same reference clock, and undergo nearly identical propagation paths to the receiver, resulting in strong coherence between the two signals. Joint processing of these signals can achieve optimal measurement performance due to the increased Gabor bandwidth and power. In this paper, we propose a universal scheme of asymmetric dual-band tracking (ASYM-DBT) to take advantage of the strong coherence, the increased Gabor bandwidth, and power of the two signals in achieving much-reduced thermal noise and more accurate ranging results when compared with the traditional single-band algorithm.

  8. Competition for vitamin B1 (thiamin) structures numerous ecological interactions.

    Science.gov (United States)

    Kraft, Clifford E; Angert, Esther R

    2017-06-01

    Thiamin (vitamin B1) is a cofactor required for essential biochemical reactions in all living organisms, yet free thiamin is scarce in the environment. The diversity of biochemical pathways involved in the acquisition, degradation, and synthesis of thiamin indicates that organisms have evolved numerous ecological strategies for meeting this nutritional requirement. In this review we synthesize information from multiple disciplines to show how the complex biochemistry of thiamin influences ecological outcomes of interactions between organisms in environments ranging from the open ocean and the Australian outback to the gastrointestinal tract of animals. We highlight population and ecosystem responses to the availability or absence of thiamin. These include widespread mortality of fishes, birds, and mammals, as well as the thiamin-dependent regulation of ocean productivity. Overall, we portray thiamin biochemistry as the foundation for molecularly mediated ecological interactions that influence survival and abundance of a vast array of organisms.

  9. Stability of Aflatoxin B1 and Ochratoxin A in Brewing

    Science.gov (United States)

    Chu, F. S.; Chang, C. C.; Ashoor, Samy H.; Prentice, N.

    1975-01-01

    The stability of aflatoxin B1 and ochratoxin A in brewing was investigated by adding the purified toxins to the raw materials at 1 and 10 μg/g levels during mashing in a conventional micro-brewing process. The results indicate that both toxins are stable to heat and are insensitive to cooker mash treatment. Both mycotoxins were partially removed in the mashing and brewing processes. About 14 to 18% and 27 to 28% of the added toxins were found in the final beers brewed from starting materials containing 1 and 10 μg, respectively, of either toxin per g. The possible route of transmission of mycotoxins into beer is discussed. PMID:1115503

  10. Transversal modulation ion mobility spectrometry (IMS) coupled with mass spectrometry (MS): exploring the IMS-IMS-MS possibilities of the instrument.

    Science.gov (United States)

    Vidal-de-Miguel, G; Macía, M; Barrios, C; Cuevas, J

    2015-02-03

    A prototype is introduced based on the transversal modulation ion mobility spectrometry (TMIMS) technique, which provides a continuous output of mobility-selected ions, greatly easing the synchronization between different analyzing stages. In the new architecture, two stages of filtration are used to drastically reduce the background produced by one stage alone. Two-stages TMIMS was coupled with two different atmospheric pressure interface mass spectrometers (MS). The new system enables IMS-IMS-MS analysis and other modes of operation: IMS prefiltration, IMS-IMS, and full transmission mode. It provides a resolving power R > 60 in IMS mode, and R > 40 in each stage of IMS-IMS mode. 2-Propanol vapors were introduced in one of the stages to enhance the mobility variations, and their effect was studied on a set of tetraalkylammonium ions. We found that concentrations as low as 1% (in partial pressure) produce mobility variations as high as 20%, which suggest that IMS-IMS separation using dried N2 (in one stage) and a dopant (in the other stage), could be a very powerful way to enhance the separation capacity of the IMS-IMS prefiltration approach.

  11. E2eUberIM

    DEFF Research Database (Denmark)

    Wac, Katarzyna; Cummings, Mark; Dey, Jayanta

    2016-01-01

    compose new innovative services, leads to high and exponentially growing integration expenses, and impairs the user experience for deployed services. All this is reducing long-term TNO profits. To meet this challenge, solutions employing network function virtualization (NFV) and software defined......, high-quality services, we propose an innovative end-to-end service management framework called e2eUberIM. This framework contains a scalable, flexible, and volatile information model (UberIM) and a real-time, "organic" communication and storage process (e2eUber). It leverages the proprietary interfaces...... of different vendors, enabling the resulting enhanced operations system environment to automatically and in real-time optimize operational overhead while maximizing the user experience, and quickly field new innovative, composed "any-services" (XaaS). We document the e2eUberIM requirements and its design...

  12. Current status of IMS plasma wave research

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, R.R.

    1982-08-01

    Much progress has been made in plasma wave research as a result of the International Magnetospheric Study (IMS) in all areas from ground-based studies to multiple-satellite observations. Studies in all the areas have contributed to better understanding of the propagation and generation of magnetospheric plasma waves and the impact the waves have on other magnetospheric phenomena. Plasma wave measurements have been used as both local and remote sensing determinations of plasma parameters. The role of plasma waves at the various magnetospheric boundaries has been extensively studied using the IMS sources. Plamsa wave observations during the IMS have tested many existing theories and led to the foundation of many new theories. Several suggestions for future directions of plamsa wave research are offered.

  13. Midlatitude magnetometer chains during the IMS

    International Nuclear Information System (INIS)

    Mcpherron, R.L.

    1982-01-01

    The International Magnetospheric Study (IMS) is an international program to study global problems of magnetospheric dynamics. A key element of the U.S. participation in this program was the establishment of a ground magnetometer network. This network included a number of arrays at high and low latitudes. This report describes three chains established at midlatitudes, including the IMS Midlatitude Chain, the AFGL Magnetometer Network, and the Bell Lab Conjugate Array. Descriptions of the type of equipment, station locations, types of data display, and availability of data for each chain are presented in this report. A major problem of the data analysis phase of the IMS will be reducing selected subsets of these data to a common format. Currently, there are no plans to do this in a systematic manner

  14. Radiological information management system (RadIMS)

    Energy Technology Data Exchange (ETDEWEB)

    Oesterling, R.G.; Marko, S.A.; Tschaeche, A.N.

    1991-08-19

    Westinghouse Idaho Nuclear Company, Inc. (WINCO) is developing and implementing an information management system, known as RadIMS, to track and record personnel exposure to ionizing radiation. RadIMS has been designed to fulfill all the requirements of US Department of Energy (USDOE) Order 5480.11, ``Radiation Protection for Occupational Workers.`` This Order requires the contractor to maintain detailed radiation exposure records on all individuals who work at the facility. These records must be retrievable for the entire working life of the individual and be available to other USDOE contractors on request. To meet these general needs, RadIMS provides for retrieval of detailed, comprehensive individual exposure histories as well as the usual online interactions to accomplish day-today radiation protection operations. These two extremes of functionality require different approaches in the WINCO computing environment. The exposure histories include database text, paper, microfilm, and electronic bitmaps.

  15. Radiological information management system (RadIMS)

    Energy Technology Data Exchange (ETDEWEB)

    Oesterling, R.G.; Marko, S.A.; Tschaeche, A.N.

    1991-08-19

    Westinghouse Idaho Nuclear Company, Inc. (WINCO) is developing and implementing an information management system, known as RadIMS, to track and record personnel exposure to ionizing radiation. RadIMS has been designed to fulfill all the requirements of US Department of Energy (USDOE) Order 5480.11, Radiation Protection for Occupational Workers.'' This Order requires the contractor to maintain detailed radiation exposure records on all individuals who work at the facility. These records must be retrievable for the entire working life of the individual and be available to other USDOE contractors on request. To meet these general needs, RadIMS provides for retrieval of detailed, comprehensive individual exposure histories as well as the usual online interactions to accomplish day-today radiation protection operations. These two extremes of functionality require different approaches in the WINCO computing environment. The exposure histories include database text, paper, microfilm, and electronic bitmaps.

  16. Organic anion transporter 3- and organic anion transporting polypeptides 1B1- and 1B3-mediated transport of catalposide

    Directory of Open Access Journals (Sweden)

    Jeong HU

    2015-01-01

    Full Text Available Hyeon-Uk Jeong,1 Mihwa Kwon,2 Yongnam Lee,3 Ji Seok Yoo,3 Dae Hee Shin,3 Im-Sook Song,2 Hye Suk Lee1 1College of Pharmacy, The Catholic University of Korea, Bucheon 420-743, Korea; 2College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea; 3Central R&D Institute, Yungjin Pharm Co., Ltd., Suwon 443-270, Korea Abstract: We investigated the in vitro transport characteristics of catalposide in HEK293 cells overexpressing organic anion transporter 1 (OAT1, OAT3, organic anion transporting polypeptide 1B1 (OATP1B1, OATP1B3, organic cation transporter 1 (OCT1, OCT2, P-glycoprotein (P-gp, and breast cancer resistance protein (BCRP. The transport mechanism of catalposide was investigated in HEK293 and LLC-PK1 cells overexpressing the relevant transporters. The uptake of catalposide was 319-, 13.6-, and 9.3-fold greater in HEK293 cells overexpressing OAT3, OATP1B1, and OATP1B3 transporters, respectively, than in HEK293 control cells. The increased uptake of catalposide via the OAT3, OATP1B1, and OATP1B3 transporters was decreased to basal levels in the presence of representative inhibitors such as probenecid, furosemide, and cimetidine (for OAT3 and cyclosporin A, gemfibrozil, and rifampin (for OATP1B1 and OATP1B3. The concentration-dependent OAT3-mediated uptake of catalposide revealed the following kinetic parameters: Michaelis constant (Km =41.5 µM, maximum uptake rate (Vmax =46.2 pmol/minute, and intrinsic clearance (CLint =1.11 µL/minute. OATP1B1- and OATP1B3-mediated catalposide uptake also showed concentration dependency, with low CLint values of 0.035 and 0.034 µL/minute, respectively. However, the OCT1, OCT2, OAT1, P-gp, and BCRP transporters were apparently not involved in the uptake of catalposide into cells. In addition, catalposide inhibited the transport activities of OAT3, OATP1B1, and OATP1B3 with half-maximal inhibitory concentration values of 83, 200, and 235 µ

  17. B1-control receive array coil (B-RAC) for reducing B1+ inhomogeneity in abdominal imaging at 3T-MRI

    Science.gov (United States)

    Kaneko, Yukio; Soutome, Yoshihisa; Habara, Hideta; Bito, Yoshitaka; Ochi, Hisaaki

    2018-02-01

    B1+ inhomogeneity in the human body increases as the nuclear magnetic resonance (NMR) frequency increases. Various methods have thus been developed to reduce B1+ inhomogeneity, such as a dielectric pad, a coupling coil, parallel transmit, and radio-frequency (RF) shimming. However, B1+ inhomogeneity still remains in some cases of abdominal imaging. In this study, we developed a B1-control receive array coil (B-RAC). Unlike the conventional receive array coil, B-RAC reduces B1+ inhomogeneity by using additional PIN diodes to generate the inductive loop during the RF transmit period. The inductive loop can generate dense and sparse regions of the magnetic flux, which can be used to compensate for B1+ inhomogeneity. First, B-RAC is modeled in the numerical simulation, and the spatial distributions of B1+ in a phantom and a human model were analyzed. Next, we fabricated a 12-channel B-RAC and measured receive sensitivity and B1+ maps in a 3T-MRI experiment. It was demonstrated that B-RAC can reduce B1+ inhomogeneity in the phantom and human model without increasing the maximum local specific absorption rate (SAR) in the body. B-RAC was also found to have almost the same the receive sensitivity as the conventional receive coil. Using RF shimming combined with B-RAC was revealed to more effectively reduce B1+ inhomogeneity than using only RF shimming. Therefore, B-RAC can reduce B1+ inhomogeneity while maintaining the receive sensitivity.

  18. IMS Seismic and Infrasound Stations Instrumental Challenges

    Science.gov (United States)

    Starovoit, Y. O.; Dricker, I. G.; Marty, J.

    2016-12-01

    The IMS seismic network is a set of monitoring facilities including 50 primary stations and 120 auxiliary stations. Besides the difference in the mode of data transmission to the IDC, technical specifications for seismographic equipment to be installed at both types of stations are essentially the same. The IMS infrasound network comprises 60 facilities with the requirement of continuous data transmission to IDC. The objective of this presentation is to report instrumental challenges associated with both seismic and infrasound technologies. In context of specifications for IMS seismic stations it was stressed that verification seismology is concerned with searching of reliable methods of signal detections at high frequencies. In the meantime MS/mb screening criteria between earthquakes and explosions relies on reliable detection of surface waves. The IMS seismic requirements for instrumental noise and operational range of data logger are defined as certain dB level below minimum background within the required frequency band from 0.02 to 16Hz. The type of sensors response is requested to be flat either in velocity or acceleration. The compliance with IMS specifications may thus introduce a challenging task when low-noise conditions have been recorded at the site. It means that as a station noise PSD approaches the NLNM it requires a high sensitive sensor to be connected to a quiet digitizer which may cause a quick system clip and waste of the available dynamic range. The experience has shown that hybrid frequency response of seismic sensors where combination of flat to velocity and flat to acceleration portions of the sensor frequency response may provide an optimal solution for utilization of the dynamic range and low digitizer noise floor. Vast efforts are also being undertaken and results achieved in the infrasound technology to standardize and optimize the response of the Wind-Noise Reduction System within the IMS infrasound passband from 0.02-4Hz and to deploy

  19. Chronic effects of fumonisin B1 on ducks.

    Science.gov (United States)

    Tran, S T; Auvergne, A; Benard, G; Bailly, J D; Tardieu, D; Babilé, R; Guerre, P

    2005-01-01

    Partially purified fumonisin B1 (FB1) was orally administrated for 77 d to 5 groups of 8 mule ducks starting at 7 d of age; the concentrations corresponded to 5 diets containing 0, 2, 8, 32, and 128 mg of FB1/kg of feed. No mortality was observed, and no effects on feed consumption and body weight gain were observed at the end of the treatment period. But, surprisingly, FB1 ingested at 32 and 128 mg/kg led to decreased body weight from d 28 to 63 and from d 7 to 63, respectively. FB1 had no effect on the relative weight of heart and breast muscle, whereas a significant increases in the relative weights of gizzard, spleen, and liver were measured in ducks receiving 32 and 128 mg of FB1/kg of feed without evidence of detectable microscopic modification of these organs. FB1 had no significant effect of the serum aspartate aminotransferase and gamma-glutamyltransferase levels but increased serum total protein, cholesterol, alanine aminotransferase, lactate dehydrogenase, alkaline phosphatase levels when 128 mg of FB1/kg of feed was given. Serum, liver, and kidney sphinganine to sphingosine ratio was significantly increased in ducks fed 8 to 128 mg of FB1/kg of feed. The biggest increase was observed in kidneys, suggesting that this organ is the most sensitive to detect FB1-induced disruption of sphingolipid metabolism.

  20. Identification of New Aflatoxin B1-Degrading Bacteria from Iran

    Directory of Open Access Journals (Sweden)

    Fahimeh Sangi

    2018-04-01

    Full Text Available Background: Aflatoxin B1 (AFB1 is a mutagenic and carcinogenic compound mainly produced by the Aspergillus parasiticus, A. flavus, A. nomius, A. tamari, and A. pseudotamarii. AFB1 biodegradation is the most important strategy for reducing AFB1 in plant tissues. Bacteria can deactivate and biodegrade AFB1 for effective detoxification of contaminated products. The present study investigated the efficiency of AFB1 degradation by soil bacteria from the Southern Khorasan Province in Eastern Iran by thin-layer and high-performance liquid chromatography during 2014–2015. Methods: DNA was extracted from AFB1-degrading isolates by the cetyltrimethylammonium bromide method and the 16S rRNA gene was amplified with the 27f and 1492r general bacterial primers and the sequences were used to identify the isolates based on their similarity to Gene Bank sequences of known bacterial species. Results: We isolated five strains from four species of AFB1-degrading bacteria from Birjand plain, including Bacillus pumilus, two isolates of Ochrobactrum pseudogrigonens, Pseudomonas aeruginosa, and Enterobacter cloace, which had AFB1-degrading activities of 88%, 78%, 61%, 58%, and 51%, respectively. Conclusion: We provide the first demonstration of AFB1 degradation by B. pumilus in from Iran and the first report identifying O. pseudogrigonens and E. cloace species as having AFB1-degrading activity.

  1. B-1 cell immunoglobulin directed against oxidation-specific epitopes

    Directory of Open Access Journals (Sweden)

    Dimitrios eTsiantoulas

    2013-01-01

    Full Text Available Natural antibodies (NAbs are pre-existing antibodies with germline origin that arise in the absence of previous exposure to foreign antigens. NAbs are produced by B-1 lymphocytes and are primarily of the IgM isotype. There is accumulating evidence that - in addition to their role in antimicrobial host defense - NAbs exhibit important housekeeping functions by facilitating the non-immunogenic clearance of apoptotic cells as well as the removal of (neo-self antigens. These properties are largely mediated by the ability of NAbs to recognize highly conserved and endogenously generated structures, which are exemplified by so-called oxidation-specific epitopes (OSEs that are products of lipid peroxidation. The generation of OSEs as well as their interaction with the immune system have been studied extensively in the context of atherosclerosis, a chronic inflammatory disease of the vascular wall that is characterized by the accumulation of cellular debris and oxidized low-density lipoproteins (OxLDL. Both apoptotic cells as well as OxLDL carry OSEs that are targeted by NAbs. Therefore, OSEs represent stress-induced neo-self structures that mediate recognition of metabolic waste (e.g. cellular debris by NAbs, allowing its safe disposal, which has fundamental implications in health and disease.

  2. Decomposition and detoxification of aflatoxin B1 by lactic acid.

    Science.gov (United States)

    Aiko, Visenuo; Edamana, Prasad; Mehta, Alka

    2016-04-01

    A degradation study of aflatoxin B1 (AFB1) was carried out using a combination of physical and chemical methods. AFB1 was heated at 80 °C in the presence of acetic, citric and lactic acids for various time periods. The cytotoxicity of the degraded AFB1 and its products were determined by MTT assay. The results showed that among the three organic acids lactic acid was most efficient in degrading AFB1. Although complete degradation was not observed, up to 85% degradation of AFB1 was obtained when heated for 120 min. Degradation of AFB1 was confirmed by the reduced toxicity on HeLa cells using MTT assay. Treatment with lactic acid resulted in the conversion of AFB1 into two degradation products. These products were observed at lower retention factors of 0.63 and 0.38, which were identified as AFB2 and AFB2a, respectively. The cytotoxicity of AFB2a exhibited much reduced toxicity on HeLa cells compared to that of AFB1. The results have shown the efficiency of lactic acid in degrading AFB1. This study suggest that lactic acid may be considered for use in the food and feed industry since it is present naturally in food and is considered safe. © 2015 Society of Chemical Industry.

  3. SLC9B1 methylation predicts fetal intolerance of labor.

    Science.gov (United States)

    Knight, Anna K; Conneely, Karen N; Kilaru, Varun; Cobb, Dawayland; Payne, Jennifer L; Meilman, Samantha; Corwin, Elizabeth J; Kaminsky, Zachary A; Dunlop, Anne L; Smith, Alicia K

    2018-01-01

    Fetal intolerance of labor is a common indication for delivery by Caesarean section. Diagnosis is based on the presence of category III fetal heart rate tracing, which is an abnormal heart tracing associated with increased likelihood of fetal hypoxia and metabolic acidemia. This study analyzed data from 177 unique women who, during their prenatal visits (7-15 weeks and/or 24-32 weeks) to Atlanta area prenatal care clinics, consented to provide blood samples for DNA methylation (HumanMethylation450 BeadChip) and gene expression (Human HT-12 v4 Expression BeadChip) analyses. We focused on 57 women aged 18-36 (mean 25.4), who had DNA methylation data available from their second prenatal visit. DNA methylation patterns at CpG sites across the genome were interrogated for associations with fetal intolerance of labor. Four CpG sites (P value intolerance of labor. DNA methylation and gene expression were negatively associated when examined longitudinally during pregnancy using a linear mixed-effects model. Positive predictive values of methylation of these four sites ranged from 0.80 to 0.89, while negative predictive values ranged from 0.91 to 0.92. The four CpG sites were also associated with fetal intolerance of labor in an independent cohort (the Johns Hopkins Prospective PPD cohort). Therefore, fetal intolerance of labor could be accurately predicted from maternal blood samples obtained between 24-32 weeks gestation. Fetal intolerance of labor may be accurately predicted from maternal blood samples obtained between 24-32 weeks gestation by assessing DNA methylation patterns of SLC9B1. The identification of pregnant women at elevated risk for fetal intolerance of labor may allow for the development of targeted treatments or management plans.

  4. Aflatoxin B1 Degradation by a Pseudomonas Strain

    Directory of Open Access Journals (Sweden)

    Lancine Sangare

    2014-10-01

    Full Text Available Aflatoxin B1 (AFB1, one of the most potent naturally occurring mutagens and carcinogens, causes significant threats to the food industry and animal production. In this study, 25 bacteria isolates were collected from grain kernels and soils displaying AFB1 reduction activity. Based on its degradation effectiveness, isolate N17-1 was selected for further characterization and identified as Pseudomonas aeruginosa. P. aeruginosa N17-1 could degrade AFB1, AFB2 and AFM1 by 82.8%, 46.8% and 31.9% after incubation in Nutrient Broth (NB medium at 37 °C for 72 h, respectively. The culture supernatant of isolate N17-1 degraded AFB1 effectively, whereas the viable cells and intra cell extracts were far less effective. Factors influencing AFB1 degradation by the culture supernatant were investigated. Maximum degradation was observed at 55 °C. Ions Mn2+ and Cu2+ were activators for AFB1 degradation, however, ions Mg2+, Li+, Zn2+, Se2+, Fe3+ were strong inhibitors. Treatments with proteinase K and proteinase K plus SDS significantly reduced the degradation activity of the culture supernatant. No degradation products were observed based on preliminary LC-QTOF/MS analysis, indicating AFB1 was metabolized to degradation products with chemical properties different from that of AFB1. The results indicated that the degradation of AFB1 by P. aeruginosa N17-1 was enzymatic and could have a great potential in industrial applications. This is the first report indicating that the isolate of P. aeruginosa possesses the ability to degrade aflatoxin.

  5. "Excuse me" and "I'm sorry."

    Science.gov (United States)

    Borkin, Ann; Reinhart, Susan M.

    1979-01-01

    Examines an aspect of colloquial American English in which linguistics and area studies are involved. Analyzes typical errors which lead to misunderstandings and discusses in detail the use of the expressions "excuse me" and "I'm sorry." (IFS/WGA)

  6. Vorberufliche und berufliche Bildung im internationalen Vergleich

    OpenAIRE

    Lauterbach, Uwe

    1987-01-01

    Dieser Artikel behandelt die Themen: Übergang vom Schul- in das Ausbildungs- und Beschäftigungssystem, Vorberufliche Bildung in der Sekundarschule, Berufliche Erstausbildung in den Sekundarschulen in alternierender Form oder durch die Wirtschaft, Typisierung der vorberuflichen und beruflichen Bildung im internationalen Vergleich. (DIPF/Orig.)

  7. Aan de slag met IMS Learning Design

    NARCIS (Netherlands)

    Tattersall, Colin

    2005-01-01

    This presentation (in Dutch) was given at the SURF SiX Educatieve Content meeting in Eindhoven on March 22nd 2005. It gives a brief overview of IMS LD before examining the current state of the art in tooling. Particular attention is given to RELOAD and CopperCore. The presentation is concluded with

  8. Geschlechterstereotype im Einsatz Gender Stereotypes in Action

    Directory of Open Access Journals (Sweden)

    Erika Kegyes

    2007-11-01

    Full Text Available Die umfangreiche Monographie gehört zum Forschungsbereich der kritischen angewandten Linguistik (Critical Applied Linguistics und liegt sowohl theoretisch als auch praktisch im Schnittpunkt der gender- und werbesprachlichen Untersuchungen.The extensive monograph belongs to the field of critical applied linguistics and is located both theoretically and practically at the intersection of studies of gender and advertising language.

  9. IMS Learning Design: De stand van zaken

    NARCIS (Netherlands)

    Tattersall, Colin; Manderveld, Jocelyn

    2005-01-01

    Tattersall, C. & Manderveld, J. (2004) IMS Learning Design: De stand van zaken In: Gorissen, P., Manderveld, J., Benneker, F. & Cordewener, B. Leertechnologie in de Lage Landen (pp. 31-33). Utrecht, Stichting Surf. Ook beschikbaar in dspace: http://hdl.handle.net/1820/270

  10. An IMS testbed for SIP applications

    DEFF Research Database (Denmark)

    Caba, Cosmin Marius; Soler, José

    2013-01-01

    The paper presents the design and implementation of an emulation platform for the IP Multimedia Subsystem. The SIP Servlet API v1.1 has been used to implement the final system. The purpose of the emulation is to offer to IMS service developers an environment where they can integrate development...

  11. Innovationspolitik im 6. Kondratieff: Hinterherlaufen oder vorauseilen?

    OpenAIRE

    Siemon, Cord

    2007-01-01

    Akzeptiert man die Theorie der langen Kondratieff-Wellen, so befinden wir uns gegenwärtig im 6. Kondratieff, der auf der Bio- und Nanotechnologie basiert. Wie kann die Volkswirtschaft aus evolutionsökonomischer Sicht an der Kondratieff-Dynamik partizipieren? Welche Rolle kommt dabei der Innovationspolitik zu?

  12. Systeme im Einsatz. Lernmanagement, Kompetenzmanagement und PLE

    NARCIS (Netherlands)

    Kalz, Marco; Schön, Sandra; Lindner, Martin; Roth, Detlev; Baumgartner, Peter

    2011-01-01

    Kalz, M., Schön, S., Lindner, M., Roth, D., & Baumgartner, P. (2011). Systeme im Einsatz. Lernmanagement, Kompetenzmanagement und PLE. In M. Ebner, & S. Schön (Eds.), L3T - Lerhbuch für Lernen und Lehren mit Technologie (pp. 111-118). Graz, Austria: Uni Graz. Available at

  13. Landschaftsökologische Analysen im Königsseeeinzugsgebiet

    OpenAIRE

    Vetter, Mark

    2003-01-01

    Unter der Annahme, dass Anzeichen von Umweltveränderungen durch den Klimawandel besonders in sensiblen, wenig genutzten Hochgebirgsökosystemen gut erkennbar sind, ist im Einzugsgebiet des Königssees im Nationalpark Berchtesgaden ein landschaftsökologisches Projekt unter dem Titel „Landschaftsökologische Analysen im Königsseeeinzugsgebiet“ (LAKE) durchgeführt worden. Hauptziel war die Erfassung, Darstellung und das Ergründen von Ursachen dieser Umweltveränderungen im Energie- und Stof...

  14. Determination of fumonisin B1levels in body fluids and hair from piglets fed fumonisin B1-contaminated diets.

    Science.gov (United States)

    Souto, Pollyana C M C; Jager, Alessandra V; Tonin, Fernando G; Petta, Tânia; Di Gregório, Mayra C; Cossalter, Anne-Marie; Pinton, Philippe; Oswald, Isabelle P; Rottinghaus, George E; Oliveira, Carlos A F

    2017-10-01

    The levels of fumonisin B 1 (FB 1 ) residues in plasma, urine, feces and hair from 24 piglets fed FB 1 -contaminated diets containing 3.1, 6.1 or 9.0 μg FB 1 .g -1 for 28 days were determined using liquid chromatography coupled to mass spectrometry (LC-MS/MS). The levels of FB 1 in plasma, urine, feces and pooled hair (n = 3) samples varied from 0.15 to 1.08 μg.L -1 , 16.09-75.01 μg.L -1 , 1.87-13.89 μg.g -1 and 2.08-8.09 ng.g -1 , respectively. Significant correlations (r = 0.808-0.885; P hair samples and FB 1 intake. Plasma and urinary FB 1 are good biomarkers of early exposure of pigs to low dietary FB 1 levels, although plasma is recommended to assess prolonged exposure (>14 days). The possibility to evaluate hair as a biomarker of fumonisin exposure was established, although further studies are needed to provide physiologically based toxicokinetics of residual FB 1 in the pig hair. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Flexible authoring and delivery of online courses using IMS LD

    NARCIS (Netherlands)

    Hermans, Henry; Janssen, José; Koper, Rob

    2014-01-01

    Since the publication of the IMS Learning Design (IMS LD) specification in 2003 many initiatives have been undertaken to build authoring tools that are simple enough to be used by non-technical instructors and teachers. IMS LD’s technical complexity is believed to be a major burden for the adoption

  16. [Kriegsland im Osten Eroberung, Kolonisierung und Militärherrschaft im Ersten Weltkrieg] / Markus Pöhlmann

    Index Scriptorium Estoniae

    Pöhlmann, Markus

    2003-01-01

    Rets.: Vejas Gabriel Liulevicius. Kriegsland im Osten Eroberung, Kolonisierung und Militärherrschaft im Ersten Weltkrieg. Aus dem Amerikanischen von Jürgen Bauer, Edith Nerke und Fee Engemann. Hamburg : 2002

  17. CO-OCCURRENCE OF AFLATOXIN B1, FUMONISIN B1, OCHRATOXIN A AND ZEARALENONE IN CEREALS AND PEANUTS IN COTE D'IVOIRE

    OpenAIRE

    SANGARE-TIGORI, Béatrice; MOUKHA, Serge; KOUADIO, James; BETBEDER, Anne-Marie; DANO, Sébastien; CREPPY, Edmond E

    2006-01-01

    Abstract The present survey examined 30 samples of rice (n=10), maize (n=10) and peanuts (n=10) from Cote d?Ivoire for aflatoxin B1, fumonisin B1 and zearalenone using immuno-assays, and ochratoxinA using a validated HPLC-florimetric method. Similarly to some other countries it appeared that in Cote d?Ivoire several mycotoxins are present in the same commodities. These mycotoxins are from different structural families: aflatoxin B1, fumonisin B1, zearalenone, and ochratoxin A. T...

  18. PENGARUH KETEBALAN SUBSTRAT PADA FERMENTASI TEMPE TERHADAP KADAR VITAMIN B1 (THE INFLUENCE OF SUBSTRATE THICKNESS DURING TEMPE FERMENTATION ON VITAMINS B1 LEVEL

    Directory of Open Access Journals (Sweden)

    Fifi Retiaty

    2012-12-01

    Full Text Available ABSTRACT The level of vitamin B1 in tempe could be increased by modifying the surface of substrate during soybean fermentation. This research aimed to know the influence of substrate thickness during soybean fermentation into tempe to the levels of vitamin B1. The design of this research was completed randomized design with two replication. Vitamin B1 content was analyzed on raw soybeans, boiled soybeans, and tempe using spectrophotometer methods. The substrate thicknesses examined were 0.25 cm, 0.50 cm and 1.00cm. Vitamin B1 level on raw soybean was 0.7436 mg percent and on boiled soybean was 0.4898 mg percent, while in tempe with substrate thickness  0.25 cm, 0.50 cm and 1.00 cm, the vitamin B1 contents were 1.1413, 0.9044, and 0.7130 mg percent respectively. The thickness of tempe substrate affected on vitamin B1 content. A thinner substrate resulted on higher vitamin B1 level. Keywords: fermentation, tempe, vitamin B1   ABSTRAK Kadar vitamin B1 pada tempe dapat ditingkatkan dengan memodifikasi permukaan dari substrat dalam fermentasi kedelai. Tujuan penelitian ini adalah untuk mengetahui pengaruh ketebalan substrat pada fermentasi kedelai menjadi tempe terhadap kadar vitamin B1. Disain penelitian ini adalah Rancangan Acak Lengkap dengan dua kali ulangan. Kadar vitamin B1 dianalisis pada kedelai mentah, kedelai rebus, dan tempe dengan menggunakan metode spektrofotometri. Ketebalan substrat yang diteliti adalah 0.25 cm, 0,50 cm, 1,00 cm. Kadar vitamin B1 pada  kedelai mentah adalah 0,7436 mg persen dan pada kedelai rebus adalah 0.4898 mg persen. Sedangkan kadar vitamin B1 pada tempe dengan ketebalan 0,25, 0,5 dan 1 cm secara berturut-turut adalah 1,1413 mg persen , 0,9044 mg persen dan 0,7130 mg persen. Ketebalan substar tempe berpengaruh pada kandungan vitamin B1. Semakin tipis substrat, akan menghasilkan vitamin B1 yang semakin tinggi. [Penel Gizi Makan 2012, 35(2: 182-188]   Kata Kunci: fermentasi, tempe, vitamin B1

  19. The PGE2/IL-10 Axis Determines Susceptibility of B-1 Cell-Derived Phagocytes (B-1CDP to Leishmania major Infection.

    Directory of Open Access Journals (Sweden)

    Angélica F Arcanjo

    Full Text Available B-1 cells can be differentiated from B-2 cells because they are predominantly located in the peritoneal and pleural cavities and have distinct phenotypic patterns and activation properties. A mononuclear phagocyte derived from B-1 cells (B-1CDP has been described. As the B-1CDP cells migrate to inflammatory/infectious sites and exhibit phagocytic capacity, the microbicidal ability of these cells was investigated using the Leishmania major infection model in vitro. The data obtained in this study demonstrate that B-1CDP cells are more susceptible to infection than peritoneal macrophages, since B-1CDP cells have a higher number of intracellular amastigotes forms and consequently release a larger number of promastigotes. Exacerbated infection by L. major required lipid bodies/PGE2 and IL-10 by B-1CDP cells. Both infection and the production of IL-10 were decreased when PGE2 production was blocked by NSAIDs. The involvement of IL-10 in this mechanism was confirmed, since B-1CDP cells from IL-10 KO mice are more competent to control L. major infection than cells from wild type mice. These findings further characterize the B-1CDP cells as an important mononuclear phagocyte that plays a previously unrecognized role in host responses to L. major infection, most likely via PGE2-driven production of IL-10.

  20. The Application of Internal Marketing (IM in a Service Organization

    Directory of Open Access Journals (Sweden)

    R. Sharma

    2013-07-01

    Findings and Strategic Implications. To complement earlier theoretical proposition on IM, the findings indicate that there are several empirical aspects such as “costs, people and concept” which hinder successful implementation of IM. This study proposes several strategic HR recommendations to reduce the “implementation gap” in IM such as to conduct continuous staff training on IM knowledge on regular time intervals, to reduce excessive work load and pressure and engaging staff members in continuous personal development sessions. In addition, the findings reveal that IM training should be given priority to solve “people-oriented” issues.

  1. Defining the Nation: National Identity in South Sudanese Media Discourse Eine Nation definieren: Die nationale Identität im südsudanesischen Mediendiskurs

    Directory of Open Access Journals (Sweden)

    Ole Frahm

    2012-01-01

    Full Text Available This article examines debates about national identity in the media landscape of post-referendum and post-independence South Sudan. Having never existed as a sovereign state and with its citizens being a minority group in Sudan, collective action among South Sudanese has historically been shaped in response to external pressures: in particular, the aggressive nation-building pursued by successive Khartoum governments that sought to Arabize and Islamize the South. Today, in the absence of a clear-cut enemy, it is a major challenge for South Sudan to devise a common identity that unites the putative nation beyond competing loyalties to ethnicity, tribe and family. Analysing opinion pieces from South Sudanese online media and placing them in the context of contemporary African nationalism, this article gives an initial overview of the issues that dominate the public debate on national identity: fear of tribalism and regionalism, commemoration of the liberation struggle, language politics, and the role of Christianity.Dieser Artikel widmet sich den Debatten, die in südsudanesischen Medien von der Nachreferendumszeit bis einige Monate nach der Unabhängigkeit über die nationale Identität geführt wurden. Der Südsudan hatte nie als souveräner Staat existiert und innerhalb des Sudan hatten die Südsudanesen eine Minderheit gebildet. In der Geschichte war gemeinschaftliches Handeln der Südsudanesen in erster Linie als Reaktion auf Druck von außen in Erscheinung getreten, insbesondere im Zusammenhang mit Arabisierungs- und Islamisierungsbestrebungen von Regierungen in Khartum. Heute fehlt ein eindeutiges Feindbild. Daher ist der Entwurf einer gemeinsamen Identität, die das Land über konkurrierende Loyalitäten zu Stamm, Ethnie oder Familie hinweg zu einen vermag, eine große Herausforderung für den Südsudan. Auf der Grundlage von Meinungsäußerungen in südsudanesischen Online-Zeitungen, die er zum „neuen“ afrikanischen

  2. Hurricane Satellite (HURSAT) from International Satellite Cloud Climatology Project (ISCCP) B1, Version 6

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Hurricane Satellite (HURSAT) from derived International Satellite Cloud Climatology Project (ISCCP) B1 observations of tropical cyclones worldwide. The B1 data...

  3. MISR Level 1B1 Local Mode Radiance Data V002

    Data.gov (United States)

    National Aeronautics and Space Administration — The MISR Local Mode Level 1B1 Product contains the data numbers (DNs) radiometrically scaled to radiances with no geometric resampling.The MISR Level 1B1 Radiance...

  4. Bücherhallen Hamburg im rechten Licht

    OpenAIRE

    Keite, Uta; Banduch, Raffael

    2015-01-01

    Die Bücherhallen Hamburg liefern ein Beispiel, auf welche Weise Bibliotheken "grüner" gestaltet werden können. Die Optimierung der Beleuchtung spielte eine entscheidende Rolle im Rahmen der Umbaumaßnahmen, um das kürzlich bezogene Kontorhaus den Anforderungen der Zentralbibliothek anzupassen. In Zusammenarbeit mit einem erfahrenen Lichtplaner und dem Architekten wurde die Beleuchtung entsprechend der Nutzung und dem Zweck der unterschiedlichen Zonen umgestaltet. Die einzelnen Bibliothek...

  5. THOR Ion Mass Spectrometer instrument - IMS

    Science.gov (United States)

    Retinò, Alessandro; Kucharek, Harald; Saito, Yoshifumi; Fraenz, Markus; Verdeil, Christophe; Leblanc, Frederic; Techer, Jean-Denis; Jeandet, Alexis; Macri, John; Gaidos, John; Granoff, Mark; Yokota, Shoichiro; Fontaine, Dominique; Berthomier, Matthieu; Delcourt, Dominique; Kistler, Lynn; Galvin, Antoniette; Kasahara, Satoshi; Kronberg, Elena

    2016-04-01

    Turbulence Heating ObserveR (THOR) is the first mission ever flown in space dedicated to plasma turbulence. Specifically, THOR will study how turbulent fluctuations at kinetic scales heat and accelerate particles in different turbulent environments within the near-Earth space. To achieve this goal, THOR payload is being designed to measure electromagnetic fields and particle distribution functions with unprecedented resolution and accuracy. Here we present the Ion Mass Spectrometer (IMS) instrument that will measure the full three-dimensional distribution functions of near-Earth main ion species (H+, He+, He++ and O+) at high time resolution (~ 150 ms for H+ , ~ 300 ms for He++) with energy resolution down to ~ 10% in the range 10 eV/q to 30 keV/q and angular resolution ~ 10°. Such high time resolution is achieved by mounting multiple sensors around the spacecraft body, in similar fashion to the MMS/FPI instrument. Each sensor combines a top-hat electrostatic analyzer with deflectors at the entrance together with a time-of-flight section to perform mass selection. IMS electronics includes a fast sweeping high voltage board that is required to make measurements at high cadence. Ion detection includes Micro Channel Plates (MCP) combined with Application-Specific Integrated Circuits (ASICs) for charge amplification, discrimination and time-to-digital conversion (TDC). IMS is being designed to address many of THOR science requirements, in particular ion heating and acceleration by turbulent fluctuations in foreshock, shock and magnetosheath regions. The IMS instrument is being designed and will be built by an international consortium of scientific institutes with main hardware contributions from France, USA, Japan and Germany.

  6. Gestaltung zeit- und sicherheitskritischer Warnungen im Fahrzeug

    OpenAIRE

    Fricke, Nicola

    2009-01-01

    Heutige Fahrzeuge beinhalten eine Reihe technischer Unterstützungsangebote, die dem Fahrer bei der Ausführung seiner Fahraufgabe und weiteren Nebentätigkeiten assistieren. In der Interaktion mit diesen Systemen können jedoch erhebliche Probleme auftreten, vor allem im Zusammenspiel der verschiedenen Funktionen. Besonders für zeit- und sicherheitskritische Information, wie z. B. bei Kollisionswarnungen, ist es unbedingt erforderlich, dass derartige Meldungen informativ, aber auch einfach und s...

  7. Toward an Intelligent Ion Mobility Spectrometer (IMS)

    Energy Technology Data Exchange (ETDEWEB)

    Timothy R. McJunkin; Jill R. Scott; Carla J. Miller

    2003-07-01

    The ultimate goal is to design and build a very smart ion mobility spectrometer (IMS) that can operate autonomously. To accomplish this, software capable of interpreting spectra so that it can be used in control loops for data interpretation as well as adjusting instrument parameters is being developed. Fuzzy logic and fuzzy numbers are used in this IMS spectra classification scheme. Fuzzy logic provides a straight forward method for developing a classification/detection system, whenever rules for classifying the spectra can be described linguistically. Instead of using 'max' and 'min' values, the product of the truth values is used to determine class membership. Using the product allows rule-bases that utilize the AND function to allow each condition to discount truth value in determining membership, while rule-bases with an OR function are allowed to accumulate membership. Fuzzy numbers allow encapsulation of the uncertainties due to ion mobility peak widths as well as measured instrumental parameters, such as pressure and temperature. Associating a peak with a value of uncertainty, in addition to making adjustments to the mobility calculation based on variations in measured parameters, enables unexpected shifts to be more reliably detected and accounted for; thereby, reducing the opportunity for 'false negative' results. The measure of uncertainty is anticipated to serve the additional purpose of diagnosing the operational conditions of the IMS instrument.

  8. Grand Prix Eurovision: Eine Fankultur im Medienzeitalter

    Directory of Open Access Journals (Sweden)

    Heinz Moser

    2017-06-01

    Full Text Available Der Grand Prix Eurovision ist seit Jahrzehnten eine der bekanntesten Unterhaltungssendungen im europäischen Raum. Dennoch erregte es Verwunderung, wenn der Schreibende Bekannten darüber berichtete, daß dies ein Forschungsgegenstand sei. Wurde von Interviews mit Grand Prix-Fans erzählt, so fielen schnell Aussagen wie: „Wie kann man sich nur für so etwas Abseitiges und Triviales wie den Grand Prix interessieren“. Dennoch bin ich der Meinung, daß Fankulturen für die entstehende Mediengesellschaft ein nicht unwichtiges Forschungsthema darstellen. Zwar geht es nicht um eine medienpädagogische Fragestellung im engeren Sinne; die Fans des Grand Prix Eurovision sind dem Jugendalter längst entwachsen. Dennoch handelt es sich bei Fangemeinschaften um Phänomene, die im Rahmen von Jugend- und Kinderkulturen von besonderer Relevanz sind. So meint Winter (1997, daß jugendliche Fanwelten eine bedeutende Rolle als Kristallisationspunkte kultureller Differenzierung spielen: ,Die Zugehörigkeit zu einer Fan weit ist Teil der jugendlichen Lebensbewältigung in der Postmoderne, denn in der Gemeinschaft der Fans können Jugendliche emotionale Allianzen eingehen, außeralltäglichen Beschäftigungen nachgehen, expressive Identitätsmuster gemeinschaftlich realisieren und sich mit ihrer Lebenssituation als Heranwachsende auseinandersetzen“ (Winter 1997, S. 51f..

  9. Toward an Intelligent Ion Mobility Spectrometer (IMS)

    International Nuclear Information System (INIS)

    McJunkin, Timothy R.; Scott, Jill R.; Miller, Carla J.

    2003-01-01

    The ultimate goal is to design and build a very smart ion mobility spectrometer (IMS) that can operate autonomously. To accomplish this, software capable of interpreting spectra so that it can be used in control loops for data interpretation as well as adjusting instrument parameters is being developed. Fuzzy logic and fuzzy numbers are used in this IMS spectra classification scheme. Fuzzy logic provides a straight forward method for developing a classification/detection system, whenever rules for classifying the spectra can be described linguistically. Instead of using 'max' and 'min' values, the product of the truth values is used to determine class membership. Using the product allows rule-bases that utilize the AND function to allow each condition to discount truth value in determining membership, while rule-bases with an OR function are allowed to accumulate membership. Fuzzy numbers allow encapsulation of the uncertainties due to ion mobility peak widths as well as measured instrumental parameters, such as pressure and temperature. Associating a peak with a value of uncertainty, in addition to making adjustments to the mobility calculation based on variations in measured parameters, enables unexpected shifts to be more reliably detected and accounted for; thereby, reducing the opportunity for 'false negative' results. The measure of uncertainty is anticipated to serve the additional purpose of diagnosing the operational conditions of the IMS instrument.

  10. Kinin B1 Receptor Promotes Neurogenic Hypertension Through Activation of Centrally Mediated Mechanisms.

    Science.gov (United States)

    Sriramula, Srinivas; Lazartigues, Eric

    2017-12-01

    Hypertension is associated with increased activity of the kallikrein-kinin system. Kinin B1 receptor (B1R) activation leads to vasoconstriction and inflammation. Despite evidence supporting a role for the B1R in blood pressure regulation, the mechanisms by which B1R could alter autonomic function and participate in the pathogenesis of hypertension remain unidentified. We sought to explore whether B1R-mediated inflammation contributes to hypertension and investigate the molecular mechanisms involved. In this study, we tested the hypothesis that activation of B1R in the brain is involved in the pathogenesis of hypertension, using the deoxycorticosterone acetate-salt model of neurogenic hypertension in wild-type and B1R knockout mice. Deoxycorticosterone acetate-salt treatment in wild-type mice led to significant increases in B1R mRNA and protein levels and bradykinin levels, enhanced gene expression of carboxypeptidase N supporting an increase in the B1R ligand, associated with enhanced blood pressure, inflammation, sympathoexcitation, autonomic dysfunction, and impaired baroreflex sensitivity, whereas these changes were blunted or prevented in B1R knockout mice. B1R stimulation was further shown to involve activation of the ASK1-JNK-ERK1/2 and NF-κB pathways in the brain. To dismiss potential developmental alterations in knockout mice, we further used B1R blockade selectively in the brain of wild-type mice. Supporting the central origin of this mechanism, intracerebroventricular infusion of a specific B1R antagonist, attenuated the deoxycorticosterone acetate-salt-induced increase in blood pressure in wild-type mice. Our data provide the first evidence of a central role for B1R-mediated inflammatory pathways in the pathogenesis of deoxycorticosterone acetate-salt hypertension and offer novel insights into possible B1R-targeted therapies for the treatment of neurogenic hypertension. © 2017 American Heart Association, Inc.

  11. Effect of vitamin B1 and mixtures of B1 with other vitamins on cytostatic efficiency of sanazole under irradiation. A study in vitro

    International Nuclear Information System (INIS)

    Heinrich, Edith; Getoff, Nikola

    2003-01-01

    Experiments in vitro, using bacteria Escherichia coli (AB 1157) as a biological model, showed that the cytostatic efficiency of sanazole (AK-2123, a nitrotriazole-type radiosensitizer) in radiation treatment can be strongly influenced by the presence of various vitamins. In airfree media the sanazole action is increased by a factor of 2.5 in the presence of vitamin (vit.) B1, vit. C E-acetate and β-carotene, whereas vit. B1 used individually possesses a 2.7-times higher cytostatic activity than sanazole itself. In media containing air the highest increase of sanazole action is observed in the presence of vit. B1 and C, whereas the individual use of vit. B1 shows a radiation protection effect. In media saturated with N 2 O the addition of the vit. B1 and C causes a 1.8-times larger sanazole activity, but the application of vit. B1 alone brings about a very high radiation protection. >From studies of vit. B1-radiolysis it can be concluded that OH radicals are the major primary transients leading to substrate degradation. The results are of interest for the radiation therapy of cancer

  12. Effect of vitamin B1 and mixtures of B1 with other vitamins on cytostatic efficiency of sanazole under irradiation. A study in vitro

    Science.gov (United States)

    Heinrich, Edith; Getoff, Nikola

    2003-06-01

    Experiments in vitro, using bacteria Escherichia coli (AB 1157) as a biological model, showed that the cytostatic efficiency of sanazole (AK-2123, a nitrotriazole-type radiosensitizer) in radiation treatment can be strongly influenced by the presence of various vitamins. In airfree media the sanazole action is increased by a factor of 2.5 in the presence of vitamin (vit.) B1, vit. C E-acetate and β-carotene, whereas vit. B1 used individually possesses a 2.7-times higher cytostatic activity than sanazole itself. In media containing air the highest increase of sanazole action is observed in the presence of vit. B1 and C, whereas the individual use of vit. B1 shows a radiation protection effect. In media saturated with N 2O the addition of the vit. B1 and C causes a 1.8-times larger sanazole activity, but the application of vit. B1 alone brings about a very high radiation protection. From studies of vit. B1-radiolysis it can be concluded that OH radicals are the major primary transients leading to substrate degradation. The results are of interest for the radiation therapy of cancer.

  13. CYP1B1 expression, a potential risk factor for breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Goth-Goldstein, Regine; Erdmann, Christine A.; Russell, Marion

    2001-05-31

    CYP1B1 expression in non-tumor breast tissue from breast cancer patients and cancer-free individuals was determined to test the hypothesis that high CYP1B1 expression is a risk factor for breast cancer. Large interindividual variations in CYP1B1 expression were found with CYP1B1 levels notably higher in breast cancer patients than cancer-free individuals. The results indicate that CYP1B1 might play a role in breast cancer either through increased PAH activation or through metabolism of endogenous estrogen to a carcinogenic derivative.

  14. Microvesicle transfer of kinin B1-receptors is a novel inflammatory mechanism in vasculitis.

    Science.gov (United States)

    Kahn, Robin; Mossberg, Maria; Ståhl, Anne-Lie; Johansson, Karl; Lopatko Lindman, Ingrid; Heijl, Caroline; Segelmark, Mårten; Mörgelin, Matthias; Leeb-Lundberg, L M Fredrik; Karpman, Diana

    2017-01-01

    During vasculitis, activation of the kinin system induces inflammation, whereby the kinin B1-receptor is expressed and activated after ligand binding. Additionally, activated blood cells release microvesicles into the circulation. Here we determined whether leukocyte-derived microvesicles bear B1-kinin receptors during vasculitis, and if microvesicles transfer functional B1-receptors to recipient cells, thus promoting inflammation. By flow cytometry, plasma from patients with vasculitis were found to contain high levels of leukocyte-derived microvesicles bearing B1-receptors. Importantly, renal biopsies from two patients with vasculitis showed leukocyte-derived microvesicles bearing B1-receptors docking on glomerular endothelial cells providing in vivo relevance. Microvesicles derived from B1-receptor-transfected human embryonic kidney cells transferred B1-receptors to wild-type human embryonic kidney cells, lacking the receptor, and to glomerular endothelial cells. The transferred B1-receptors induced calcium influx after B1-receptor agonist stimulation: a response abrogated by a specific B1-receptor antagonist. Microvesicles derived from neutrophils also transferred B1-receptors to wild-type human embryonic kidney cells and induced calcium influx after stimulation. Thus, we found a novel mechanism by which microvesicles transfer functional receptors and promote kinin-associated inflammation. Copyright © 2016 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.

  15. NOAA Climate Data Record (CDR) of Gridded Satellite Data from ISCCP B1 (GridSat-B1) Infrared Channel Brightness Temperature, Version 2

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Gridded Satellite (GridSat-B1) data provides a uniform set of quality controlled geostationary satellite observations for the visible, infrared window and...

  16. Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Camacho-Jiménez, Laura; Sánchez-Castrejón, Edna; Ponce-Rivas, Elizabeth; Muñoz-Márquez, Ma Enriqueta; Aguilar, Manuel B; Re, Ana Denisse; Díaz, Fernando

    2015-09-01

    Crustacean hyperglycemic hormone (CHH) is the most abundant neuropeptide produced by the X-organ/sinus gland (XO/SG) complex in the crustacean eyestalk. CHH plays a principal role in the control of glucose metabolism. The CHH-B1 isoform is produced in the eyestalk of Litopenaeus vannamei by alternative splicing of the chhB gene and its cDNA sequence has revealed that this isoform has a non-amidated C-terminal residue (CHH-like peptide). In this work, a recombinant CHH-B1 (rCHH-B1) with a sequence identical to the native hormone was expressed in the methylotrophic yeast Pichia pastoris X-33 and purified from the culture medium by RP-HPLC. The identity of the purified rCHH-B1 was confirmed by N-terminal sequencing and by using an anti-CHH-B1 polyclonal antibody. An in vivo assay showed that the hyperglycemic effect was dependant of the dosage of rCHH-B1, and the maximal hyperglycemic response was obtained with 250pmol treatment. These results suggest that the amino acid sequence of the C-terminus and its correct structure are both important for the hyperglycemic activity of naturally occurring non-amidated CHH peptides, such as CHH-B1. CHH-B1 appears to be the first reported CHH-like peptide with significant hyperglycemic activity produced in the sinus gland of a penaeid shrimp. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Metabolism of aflatoxin B1 and identification of the major aflatoxin B1-DNA adducts formed in cultured human bronchus and colon

    DEFF Research Database (Denmark)

    Autrup, Herman; Essigmann, John M.; Croy, Robert G.

    1979-01-01

    Aflatoxin B1 and benzo(a)pyrene were activated by both cultured human bronchus and human colon as measured by binding to cellular DNA and protein. The binding of aflatoxin B1 to DNA was dose dependent, and the level of binding was higher in cultured human bronchus than it was in the colon. When...... compared to aflatoxin B1, the binding level of benzo(a)pyrene to both bronchial and colonic DNA was generally higher. The major adducts formed in both tissues by the interaction of aflatoxin B1 and DNA were chromatographically identical to 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 (Structure 1...... in these two peaks, and the ratio of radioactivity between the peaks was nearly 1. In colonic DNA, the ratio between Structures 1 and 11 was approximately 2. These observations add aflatoxin B1 to the list of chemical procarcinogens metabolized by cultured human tissues and in which the carcinogen-DNA adducts...

  18. Architektur osmanischer Karawanseraien:Stationen des Fernverkehrs im Osmanischen Reich

    OpenAIRE

    Wimmel, Robin

    2016-01-01

    Karawanseraien, Herbergsbauten entlang von Fernrouten, stellten eine der wichtigsten Baugattungen im Orient dar. Die Hauptgruppen werden heute üblicherweise nach islamischen Dynastien unterschieden. Besonders zu Karawanseraien der Ayyubiden und Mamluken in Großsyrien, der anatolischen Seldschuken und der Safawiden im Gebiet des Iran liegen auf die Erfassung der gesamten Gruppen zielende Studien vor. Hier bestand bezüglich im Kontext osmanischer Herrschaft erbauter Karawanseraien bisher eine L...

  19. Cell stress promotes the association of phosphorylated HspB1 with F-actin.

    Directory of Open Access Journals (Sweden)

    Joseph P Clarke

    Full Text Available Previous studies have suggested that the small heat shock protein, HspB1, has a direct influence on the dynamics of cytoskeletal elements, in particular, filamentous actin (F-actin polymerization. In this study we have assessed the influence of HspB1 phosphorylation on its interaction(s with F-actin. We first determined the distribution of endogenous non-phosphorylated HspB1, phosphorylated HspB1 and F-actin in neuroendocrine PC12 cells by immunocytochemistry and confocal microscopy. We then investigated a potential direct interaction between HspB1 with F-actin by precipitating F-actin directly with biotinylated phalloidin followed by Western analyses; the reverse immunoprecipitation of HspB1 was also carried out. The phosphorylation influence of HspB1 in this interaction was investigated by using pharmacologic inhibition of p38 MAPK. In control cells, HspB1 interacts with F-actin as a predominantly non-phosphorylated protein, but subsequent to stress there is a redistribution of HspB1 to the cytoskeletal fraction and a significantly increased association of pHspB1 with F-actin. Our data demonstrate HspB1 is found in a complex with F-actin both in phosphorylated and non-phosphorylated forms, with an increased association of pHspB1 with F-actin after heat stress. Overall, our study combines both cellular and biochemical approaches to show cellular localization and direct demonstration of an interaction between endogenous HspB1 and F-actin using methodolgy that specifically isolates F-actin.

  20. Untersuchungen von UAV Technologien im DLR

    OpenAIRE

    Schütte, Andreas; Hanke, Michael

    2009-01-01

    Untersuchungen von UCAV Technologien im DLR Die Eckpunkte für die Entwicklung von UCAV-Konfigurationen definiert ein möglicher Bedarf der Luftwaffen an UCAV‘s (Unmanned Combat Air Vehicle) ab 2020. Diese Eckpunkte sind folgende: • voraussichtliche Entwicklungszeit: 8-10 Jahre • Einsatzzweck: Bekämpfung von mobilen Zielen zu Lande und in der Luft • Maximale Überlebensfähigkeit durch hohe Manövrierfähigkeit und Faserverbundleichtbau • passive Gegenmaßnahmen (minimale Signatur...

  1. Admission Control Methods in IMS Networks

    Directory of Open Access Journals (Sweden)

    Michal Cuba

    2016-01-01

    Full Text Available The article deals with solving the problem of ensuring Quality of Service (QoS in IP Multimedia Subsystem (IMS networks. Admission Control methods (AC are used to prevent network congestion and the decrease of QoS. The main function of AC is to maximize utilization of network resources and to ensure the level of QoS. Four methods were chosen for comparison. These methods are described in the main part of the article. The last part deals with simulations of these methods in the software MATLAB.

  2. Admission Control Methods in IMS Networks

    Directory of Open Access Journals (Sweden)

    Erik Chromy

    2016-09-01

    Full Text Available In this paper we present admission control methods for IMS network. The task of RACS block is to accept or reject new connection into the network. The main goal of the admission control method is to ensure the Quality of Service not only for new connection but also for already accepted connections. We discuss and compare three admission control algorithms in the paper from the qualitative parameters point of view.

  3. Modellierung von Anwenderverhalten im Social Semantic Web

    Science.gov (United States)

    Ulbrich, Armin; Höfler, Patrick; Lindstaedt, Stefanie

    Ziel dieses Kapitels ist es, gemeinsame Verwendungsszenarien des Semantic Web und des Social Web zu identifizieren und zu benennen. Dabei wird ein Teilaspekt des Themengebiets im Detail betrachtet: die Nutzung von Services, die Beobachtungen des Verhaltens von Anwendern analysieren, um daraus maschinell interpretierbare Informationen zu erhalten und diese als Modelle zu organisieren. Es werden zunächst einige Eigenschaften und Unterscheidungsmerkmale von Anwenderverhalten und organisierten Modellen dargestellt. Anschließend wird der mögliche wechselseitige Nutzen von Anwenderverhalten und Modellen diskutiert. Den Abschluss bildet eine Betrachtung einiger exemplarischer Software-Services, die heute schon verwendet werden, um Anwenderverhalten in Modelle überzuführen.

  4. Anwaltsvertragshaftung - Pflichtverletzung und Verschulden im neuen Schuldrecht

    OpenAIRE

    Karg, Thomas

    2004-01-01

    Die vorliegende Dissertation beschäftigt sich mit der vertraglichen Haftung des Anwalts und den Veränderungen, die sich durch die Schuldrechtsreform vom 01.01.2002 ergeben haben - insbesondere im Recht der Leistungsstörungen. Der Hauptteil untergliedert sich in zehn Kapitel. Zunächst wird ein kurzer Überblick über den Beruf des Anwalts einschließlich den historischen Wurzeln gegeben. Daran schließen sich die Einordnung des Anwaltsvertrages in das System des BGB sowie allgemeine und anwaltspez...

  5. Kriminologie und Kriminalistik im Zugriff der Geschichtswissenschaft

    Directory of Open Access Journals (Sweden)

    Peter-Alexis Albrecht

    2007-01-01

    Full Text Available Rezensierte Werke:Silviana Galassi, Kriminologie im Deutschen Kaiserreich. Geschichte einer gebrochenen Verwissen­schaftlichung, Stuttgart: Franz Steiner Verlag 2004, 452 S., ISBN 3-515-08352-9; Peter Becker, Dem Täter auf der Spur. Eine Geschichte der Kriminalistik, Darmstadt: Primus Verlag 2005, 287 S., ISBN 3-89678-275-4; Peter Becker, Richard F. Wetzell (Hg., Criminals and their Scientists. The History of Criminology in International Perspective, Cambridge: Cambridge University Press 2006, 492 S., ISBN 0-521-81012-4

  6. Ion Mobility Spectrometer / Mass Spectrometer (IMS-MS)

    Energy Technology Data Exchange (ETDEWEB)

    Hunka, Deborah E. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Austin, Daniel [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2005-10-01

    The use of Ion Mobility Spectrometry (IMS)in the Detection of Contraband Sandia researchers use ion mobility spectrometers for trace chemical detection and analysis in a variety of projects and applications. Products developed in recent years based on IMS-technology include explosives detection personnel portals, the Material Area Access (MAA) checkpoint of the future, an explosives detection vehicle portal, hand-held detection systems such as the Hound and Hound II (all 6400), micro-IMS sensors (1700), ordnance detection (2500), and Fourier Transform IMS technology (8700). The emphasis to date has been on explosives detection, but the detection of chemical agents has also been pursued (8100 and 6400).

  7. [Bernt Ahrenholz : Verweise mit Demonstrativa im Gesprochenen Deutsch...] / Klaus Geyer

    Index Scriptorium Estoniae

    Geyer, Klaus

    2008-01-01

    Arvustus: Ahrenholz, Bernt. Verweise mit Demonstrativa im gesprochenen Deutsch : Grammatik, Zweitspracherwerb und Deutsch als Fremdsprache. Berlin ; New York : de Gruyter, 2007. (Linguistik - Impulse & Tendenzen ; 17)

  8. The Investment Model Scale (IMS): further studies on construct validation and development of a shorter version (IMS-S).

    Science.gov (United States)

    Rodrigues, David; Lopes, Diniz

    2013-01-01

    The Investment Model (IM; Rusbult, 1980, 1983) has been widely used to study the development and maintenance of romantic relationships. Its components--satisfaction, quality of alternatives, investment size and commitment--are operationalized in the Investment Model Scale (IMS; Rusbult, Martz, & Agnew, 1998). Given its importance for personal relationships literature, this article presents the adaptation and validation of the IMS to Portugal, and the development and validation of a shorter version, the IMS-S. A confirmatory factor analysis replicates the IMS's original four factors structure. A similar structure was found for the IMS-S. For both versions, results show the instruments to have validity and good reliability. Results are discussed considering the scales' importance for studying romantic relationships.

  9. Gerichtskultur im Ostseeraum : vierter Rechtshistorikertag im Ostseeraum in Greifswald / Marc Liebmann

    Index Scriptorium Estoniae

    Liebmann, Marc

    2006-01-01

    18.-20. 05. 2005. a. toimunud 4. õigusajaloopäevast Greifswaldis, esinesid ka: Toomas Anepaio (Die zahlreichen Gesichter des Gemeindegerichts : die Entwicklung der estnischen Bauerngerichte im 19. Jahrhundert) ja Marju Luts (Der estländische Bauer vor dem höchsten Gericht des Russischen Reiches)

  10. Flavonoids exhibit diverse effects on CYP11B1 expression and cortisol synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, Li-Chuan; Li, Lih-Ann, E-mail: lihann@nhri.org.tw

    2012-02-01

    CYP11B1 catalyzes the final step of cortisol biosynthesis. The effects of flavonoids on transcriptional expression and enzyme activity of CYP11B1 were investigated using the human adrenocortical H295R cell model. All tested nonhydroxylated flavones including 3′,4′-dimethoxyflavone, α-naphthoflavone, and β-naphthoflavone upregulated CYP11B1 expression and cortisol production, whereas apigenin and quercetin exhibited potent cytotoxicity and CYP11B1 repression at high concentrations. Nonhydroxylated flavones stimulated CYP11B1-catalyzed cortisol formation at transcriptional level. Resveratrol increased endogenous and substrate-supported cortisol production like nonhydroxylated flavones tested, but it had no effect on CYP11B1 gene expression and enzyme activity. Resveratrol appeared to alter cortisol biosynthesis at an earlier step. The Ad5 element situated in the − 121/− 106 region was required for basal and flavone-induced CYP11B1 expression. Overexpression of COUP-TFI did not improve the responsiveness of Ad5 to nonhydroxylated flavones. Although COUP-TFI overexpression increased CYP11B1 and CYP11B2 promoter activation, its effect was not mediated through the common Ad5 element. Treating cells with PD98059 (a flavone-type MEK1 inhibitor) increased CYP11B1 promoter activity, but not involving ERK signaling because phosphorylation of ERK1/2 remained unvarying throughout the course of treatment. Likewise, AhR was not responsible for the CYP11B1-modulating effects of flavonoids because inconsistency with their effects on AhR activation. 3′,4′-dimethoxyflavone and 8-Br-cAMP additively activated CYP11B1 promoter activity. H-89 reduced 3′,4′-dimethoxyflavone-induced CYP11B1 promoter activation but to a lesser extent as compared to its inhibition on cAMP-induced transactivation. Our data suggest that constant exposure to nonhydroxylated flavones raises a potential risk of high basal and cAMP-induced cortisol synthesis in consequence of increased CYP11B1

  11. Highly sensitive FRET-based fluorescence immunoassay for aflatoxin B1 using cadmium telluride quantum dots

    International Nuclear Information System (INIS)

    Zekavati, Roya; Bayat, Mansour; Safi, Shahabeddin; Hashemi, Seyed Jamal; Rahmani-Cherati, Tavoos; Tabatabaei, Meisam; Mohsenifar, Afshin

    2013-01-01

    We report on a competitive immunoassay for the determination of aflatoxin B1 using fluorescence resonance energy transfer (FRET) from anti-aflatoxin B1 antibody (immobilized on the shell of CdTe quantum dots) to Rhodamine 123 (Rho 123-labeled aflatoxin B1 bound to albumin). The highly specific immuno reaction between the antibody against aflatoxin B1 on the QDs and the labeled-aflatoxin B1 brings the Rho 123 fluorophore (acting as the acceptor) and the QDs (acting as the donor) in close spatial proximity and causes FRET to occur upon photoexcitation of the QDs. In the absence of unlabeled aflatoxin B1, the antigen-antibody complex is stable, and strong emission resulting from the FRET from QDs to labeled aflatoxin B1 is observed. In the presence of aflatoxin B1, it will compete with the labeled aflatoxin B1-albumin complex for binding to the antibody-QDs conjugate so that FRET will be increasingly suppressed. The reduction in the fluorescence intensity of the acceptor correlates well with the concentration of aflatoxin B1. The feasibility of the method was established by the detection of aflatoxin B1 in spiked human serum. There is a linear relationship between the increased fluorescence intensity of Rho 123 with increasing concentration of aflatoxin B1 in spike human serum, over the range of 0.1–0.6 μmol·mL −1 . The limit of detection is 2 × 10 −11 M. This homogeneous competitive detection scheme is simple, rapid and efficient, and does not require excessive washing and separation steps. (author)

  12. Oligomerization and phase transitions in aqueous solutions of native and truncated human beta B1-crystallin.

    Science.gov (United States)

    Annunziata, Onofrio; Pande, Ajay; Pande, Jayanti; Ogun, Olutayo; Lubsen, Nicolette H; Benedek, George B

    2005-02-01

    Human betaB1-crystallin is a major eye-lens protein that undergoes in vivo truncation at the N-terminus with aging. By studying native betaB1 and truncated betaB1DeltaN41, which mimics an age-related in vivo truncation, we have determined quantitatively the effect of truncation on the oligomerization and phase transition properties of betaB1 aqueous solutions. The oligomerization studies show that the energy of attraction between the betaB1DeltaN41 proteins is about 10% greater than that of the betaB1 proteins. We have found that betaB1DeltaN41 aqueous solutions undergo two distinct types of phase transitions. The first phase transition involves an initial formation of thin rodlike assemblies, which then evolve to form crystals. The induction time for the formation of rodlike assemblies is sensitive to oligomerization. The second phase transition can be described as liquid-liquid phase separation (LLPS) accompanied by gelation within the protein-rich phase. We refer to this process as heterogeneous gelation. These two phase transitions are not observed in the case of betaB1 aqueous solutions. However, upon the addition of poly(ethylene glycol) (PEG), we observe heterogeneous gelation also for betaB1. Our PEG experiments allow us to estimate the difference in phase separation temperatures between betaB1 and betaB1DeltaN41. This difference is consistent with the increase in energy of attraction found in our oligomerization studies. Our work suggests that truncation is a cataractogenic modification since it favors protein condensation and the consequent formation of light scattering elements, and highlights the importance of the N-terminus of betaB1 in maintaining lens transparency.

  13. bildbild – visuelle Kompetenz im Unterricht

    Directory of Open Access Journals (Sweden)

    Norbert Grube

    2013-05-01

    Full Text Available Der vorliegende Artikel stellt das Webseiten-Projekt bildbild vor. Der Webseite liegt die Motivation zugrunde, Schülerinnen und Schüler für ein Nachdenken über globale Themen zu sensibilisieren. Ein solches Nachdenken ist immer auch eines über bildlich vermittelte Wirklichkeit. Es ist mit bildbild ein Anliegen, angesichts einer allseits konstatierten «Bilderflut» ein didaktisches Medium zu entwickeln, das einen Fundus an historischen und aktuellen Fotografien zur Verfügung stellt und diese so aufbereitet, dass sie gerade nicht hinter ihrer illustrativen Funktion verschwinden. Dadurch sollen die Bilder für ein selbständiges Lernen auf dem Weg hin zu einer visuellen Kompetenz einsetzbar gemacht werden. Der Beitrag beleuchtet die Entwicklung der Webseite vor dem Hintergrund kulturkritischer Haltungen zum Bild seit der Moderne sowie im Rahmen dessen, was seit den 1970er Jahren im pädagogischen Kontext als Visual Literacy diskutiert und gefördert wird. Dabei wird auch auf die medialen Potenziale einer Webseite für die Bildlesekompetenz von Jugendlichen eingegangen.

  14. Testování technologie IMS

    OpenAIRE

    Kuběna, Pavel

    2010-01-01

    Tato bakalářská práce se zabývá technologií IMS, což je standard definující architekturu pro poskytování multimediálních služeb. V teoretické části je uveden obecný popis technologie, architektura a stručný popis prvků, používané protokoly. Praktická část je věnována prostředí Open IMS Core a testování sítě navržené podle získaných teoretických znalostí. Jedná se především o popis a rozbor registračního procesu, přenosu hlasu, to znamená hovoru mezi dvěma uživateli, přenosu textu a videohovor...

  15. Im Bild sprechen: Cixous Visual Instruction: Cixous

    Directory of Open Access Journals (Sweden)

    Jens E. Sennewald

    2006-03-01

    Full Text Available Gemeinsam von Künstlern und Wissenschaftlern/Philosophen erstellte Bücher sind selten und nicht immer erfolgreich. Eines der Fährnisse ist das Verhältnis zwischen bildlichen und schriftlichen Anteilen: Nicht selten wird der künstlerische Anteil zur Illustration der dominierenden Schrift. Eine Neupositionierung des Bildes im Buch ist nun der amerikanischen Künstlerin Roni Horn gelungen. Mehr als „sujet“ ihres Buches, ist die französische Philosophin Hélène Cixous gleichsam instruierende „Stimme“, die im Bild aufscheint. Horn hat dadurch zugleich – einmal mehr – das Phantasma unmittelbarer Abbildhaftigkeit des Fotos dekonstruiert und der philosophischen Position Cixous‘ durch das Bild eine Dimension hinzugefügt.Books created through a combined effort between artists and scholars occur seldom and are not always successful. A particular imbalance is found in the relationship between image and text: often the visual components serve merely to illustrate the more dominant text. The American artist Roni Horn has finally repositioned the importance of images in books. The voice of French philosopher Hélène Cixous is not merely the “sujet” of Horn’s book, but her instructive voice appears simultaneously in “image.” Horn thus both deconstructs the chimera of the photo’s ability to immediately visually depict the real and also adds a new dimension to Cixous’ philosophic position with her images.

  16. B-1 cells produce insulin and abrogate experimental streptozotocin-induced diabetes.

    Science.gov (United States)

    Alvares-Saraiva, Anuska M; Novo, Marília C T; de Oliveira, Vivian Cristina; Maricato, Juliana T; Lopes, José Daniel; Popi, Ana Flavia; Mariano, Mario

    2015-05-01

    The participation of B-1 cells in a murine model of spontaneous diabetes has been recently reported. Here, we describe the role of B-1 cells in streptozotocin (STZ) induced diabetes in mice. We demonstrated that XID (B-1 cell-deficient) mice are more susceptible to STZ treatment than WT mice, as evidenced by their higher blood glucose level in response to STZ. Unexpectedly, the XID mice that were i.p. transferred with purified B-1 cells, either before or after the STZ treatment, did not develop diabetes. These cell transfers provided long-lasting protection for the XID mice against STZ-induced diabetes, suggesting that B-1 cells play an important role in the experimental diabetes pathobiology. We also showed that B-1 cell culture supernatants were able to regulate the blood glucose level of the diabetic XID mice, and we identified insulin-producing cells when B-1 cells were differentiated in B-1 cell-derived phagocyte in vitro. These findings provide a novel role for B-1 cells in metabolic processes, presenting a new mechanism to explain the pathogenesis of diabetes and a possible therapeutical target. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Hormonal Regulation of the Human CYP27A1 and CYP7B1 Genes

    OpenAIRE

    Tang, Wanjin

    2007-01-01

    CYP27A1 and CYP7B1 are widely expressed in various human tissues and are two key enzymes involved in the pathways for conversion of cholesterol to bile acids. Also, CYP27A1 is involved in bioactivation of vitamin D3 and CYP7B1 plays a role in 7alpha-hydroxylation of dehydroepiandrosterone and other steroids. Both enzymes have been reported to be relevant to prostate cell proliferation. The current study examines the hormonal regulation of CYP27A1 and CYP7B1. CYP7B1 was shown to be regulated b...

  18. Deutsch-slawischer Siedlungs- und Sprachkontakt im Gebiet zwischen Saale und Neiße – vorgestellt an ausgewählten Ortsnamen (Siedlungsnamen

    Directory of Open Access Journals (Sweden)

    Inge Bily

    2015-12-01

    Full Text Available Saale und Elbe bilden im Wesentlichen die westliche Begrenzung des ehemals kompakten altsorbischen Sprachgebietes. Im Norden schließt das Altsorbische an das Altpolabische, im Osten und Südosten an das Polnische und Tschechische an. Eigennamen bilden eine wichtige Quelle sowohl für die Aufhellung der Geschichte der Besiedlung wie auch ethnischer, sprachlicher und sozialer Verhältnisse, denn historische Siedlungsprozesse fanden ihren Niederschlag u.a. in historischen Belegen von Namen. Diese Belege wie auch die Ableitungsbasen und Benennungsmotive ebenso wie die phonologischen und morphologischen Merkmale der Namen des altsorbischen Kontaktgebietes enthalten eine Vielzahl von Zeugnissen deutsch-slawischer Kontinuität. Auf der Grundlage umfangreicher Studien zu Ortsnamen stellt der Beitrag ausgewählte Beispiele vor. Im ehemals altsorbischen Kontaktgebiet können Ortsnamen (Siedlungsnamen und ihre historische Überlieferung Hinweise auf Siedlungs- und Sprachkontakt geben. Dies belegen eine ganze Reihe von Merkmalen, so z.B.: 1. Unterscheidende Bestimmungswörter 2. Parallele Namengebung mit zeitweiliger Mehrnamigkeit 3. Umbenennung 4. Übersetzung 5. Benennungsparallelismus im deutsch-slawischen Kontaktgebiet 6. Scheinbare sekundäre semantische Verankerung (SSSV 7. Namenpaare 8. Unterscheidende Zusätze 9. Mischnamen (Hybride

  19. Loss of endophilin-B1 exacerbates Alzheimer’s disease pathology

    Science.gov (United States)

    Wang, David B.; Kinoshita, Yoshito; Kinoshita, Chizuru; Uo, Takuma; Sopher, Bryce L.; Cudaback, Eiron; Keene, C. Dirk; Bilousova, Tina; Gylys, Karen; Case, Amanda; Jayadev, Suman; Wang, Hong-Gang; Garden, Gwenn A.

    2015-01-01

    Endophilin-B1, also known as Bax-interacting factor 1 (Bif-1, and encoded by SH3GLB1), is a multifunctional protein involved in apoptosis, autophagy and mitochondrial function. We recently described a unique neuroprotective role for neuron-specific alternatively spliced isoforms of endophilin-B1. To examine whether endophilin-B1-mediated neuroprotection could be a novel therapeutic target for Alzheimer’s disease we used a double mutant amyloid precursor protein and presenilin 1 (APPswe/PSEN1dE9) mouse model of Alzheimer’s disease and observed that expression of neuron-specific endophilin-B1 isoforms declined with disease progression. To determine if this reduction in endophilin-B1 has a functional role in Alzheimer’s disease pathogenesis, we crossed endophilin-B1−/− mice with APPswe/PSEN1dE9 mice. Deletion of endophilin-B1 accelerated disease onset and progression in 6-month-old APPswe/PSEN1dE9/endophilin-B1−/− mice, which showed more plaques, astrogliosis, synaptic degeneration, cognitive impairment and mortality than APPswe/PSEN1dE9 mice. In mouse primary cortical neuron cultures, overexpression of neuron-specific endophilin-B1 isoforms protected against amyloid-β-induced apoptosis and mitochondrial dysfunction. Additionally, protein and mRNA levels of neuron-specific endophilin-B1 isoforms were also selectively decreased in the cerebral cortex and in the synaptic compartment of patients with Alzheimer’s disease. Flow sorting of synaptosomes from patients with Alzheimer’s disease demonstrated a negative correlation between amyloid-β and endophilin-B1 levels. The importance of endophilin-B1 in neuronal function was further underscored by the development of synaptic degeneration and cognitive and motor impairment in endophilin-B1−/− mice by 12 months. Our findings suggest that endophilin-B1 is a key mediator of a feed-forward mechanism of Alzheimer’s disease pathogenesis where amyloid-β reduces neuron-specific endophilin-B1, which in turn

  20. Targeting cyclin B1 inhibits proliferation and sensitizes breast cancer cells to taxol

    International Nuclear Information System (INIS)

    Androic, Ilija; Krämer, Andrea; Yan, Ruilan; Rödel, Franz; Gätje, Regine; Kaufmann, Manfred; Strebhardt, Klaus; Yuan, Juping

    2008-01-01

    Cyclin B1, the regulatory subunit of cyclin-dependent kinase 1 (Cdk1), is essential for the transition from G2 phase to mitosis. Cyclin B1 is very often found to be overexpressed in primary breast and cervical cancer cells as well as in cancer cell lines. Its expression is correlated with the malignancy of gynecological cancers. In order to explore cyclin B1 as a potential target for gynecological cancer therapy, we studied the effect of small interfering RNA (siRNA) on different gynecological cancer cell lines by monitoring their proliferation rate, cell cycle profile, protein expression and activity, apoptosis induction and colony formation. Tumor formation in vivo was examined using mouse xenograft models. Downregulation of cyclin B1 inhibited proliferation of several breast and cervical cancer cell lines including MCF-7, BT-474, SK-BR-3, MDA-MB-231 and HeLa. After combining cyclin B1 siRNA with taxol, we observed an increased apoptotic rate accompanied by an enhanced antiproliferative effect in breast cancer cells. Furthermore, control HeLa cells were progressively growing, whereas the tumor growth of HeLa cells pre-treated with cyclin B1 siRNA was strongly inhibited in nude mice, indicating that cyclin B1 is indispensable for tumor growth in vivo. Our data support the notion of cyclin B1 being essential for survival and proliferation of gynecological cancer cells. Concordantly, knockdown of cyclin B1 inhibits proliferation in vitro as well as in vivo. Moreover, targeting cyclin B1 sensitizes breast cancer cells to taxol, suggesting that specific cyclin B1 targeting is an attractive strategy for the combination with conventionally used agents in gynecological cancer therapy

  1. Targeting cyclin B1 inhibits proliferation and sensitizes breast cancer cells to taxol

    Directory of Open Access Journals (Sweden)

    Strebhardt Klaus

    2008-12-01

    Full Text Available Abstract Background Cyclin B1, the regulatory subunit of cyclin-dependent kinase 1 (Cdk1, is essential for the transition from G2 phase to mitosis. Cyclin B1 is very often found to be overexpressed in primary breast and cervical cancer cells as well as in cancer cell lines. Its expression is correlated with the malignancy of gynecological cancers. Methods In order to explore cyclin B1 as a potential target for gynecological cancer therapy, we studied the effect of small interfering RNA (siRNA on different gynecological cancer cell lines by monitoring their proliferation rate, cell cycle profile, protein expression and activity, apoptosis induction and colony formation. Tumor formation in vivo was examined using mouse xenograft models. Results Downregulation of cyclin B1 inhibited proliferation of several breast and cervical cancer cell lines including MCF-7, BT-474, SK-BR-3, MDA-MB-231 and HeLa. After combining cyclin B1 siRNA with taxol, we observed an increased apoptotic rate accompanied by an enhanced antiproliferative effect in breast cancer cells. Furthermore, control HeLa cells were progressively growing, whereas the tumor growth of HeLa cells pre-treated with cyclin B1 siRNA was strongly inhibited in nude mice, indicating that cyclin B1 is indispensable for tumor growth in vivo. Conclusion Our data support the notion of cyclin B1 being essential for survival and proliferation of gynecological cancer cells. Concordantly, knockdown of cyclin B1 inhibits proliferation in vitro as well as in vivo. Moreover, targeting cyclin B1 sensitizes breast cancer cells to taxol, suggesting that specific cyclin B1 targeting is an attractive strategy for the combination with conventionally used agents in gynecological cancer therapy.

  2. Thermal Degradation Study of IM7/DMBZ-15 High Temperature Composite by TGA/FTIR

    National Research Council Canada - National Science Library

    Nadler, Melvin

    2003-01-01

    (U) High temperature graphite composites such as IM7/8552 epoxy, IM7/5250 BMI, and IM7/DMBZ-15 polyimide show blistering and/or "catastrophic" delamination when rapidly heated to temperatures above...

  3. The future of the IMS Learning Design specification: a critical look

    NARCIS (Netherlands)

    Sloep, Peter

    2009-01-01

    P. B. Sloep (2009). The future of the IMS Learning Design specification: a critical look. Presentation at the IMS Learning Design seminar 'The future of IMS Learning Design'. December, 10, 2009, Wollongong, Australia: University of Wollongong.

  4. [Einheit und Vielfalt in der Rechtsgeschichte im Ostseeraum] / Marelle Leppik

    Index Scriptorium Estoniae

    Leppik, Marelle

    2013-01-01

    Arvustus: Einheit und Vielfalt in der Rechtsgeschichte im Ostseeraum. Unity and plurality in the legal history of the Baltic Sea area Sechster Rechtshistorikertag im Ostseeraum, 3.-5. Juni 2010 Tartu (Estland) / Riga (Lettland9. Hrsg. von Marju Luts-Sootak, Sanita Osipova und Frank L. Schäfer. (Rechtshistorische Reihe, Bd. 428.) Land. Frankfurt am Main u. a. 2012

  5. Translating the IM behaviour of munitions to operational consequences

    NARCIS (Netherlands)

    Hooijmeijer, P.A.; Scholtes, J.H.G.; Voort, M.M. van der

    2012-01-01

    A recent study was dedicated to relate the behaviour of Insensitive Munitions (IM) to an operational context. At TNO, a toolbox with a series of engineering tools is under study. These tools focus on the mechanisms that occur due to a range of IM threats and that transfer the effects from a reacting

  6. Information Management System (Ims) For Gender Mainstreaming In ...

    African Journals Online (AJOL)

    Information Management System (IMS) is an essential tool in supporting the provision of information needed for planning and decision making in any organization. Proper IMS facilitates the smooth flow of information both vertical and horizontal. This paper presents the findings of a survey on information needs of the Gender ...

  7. Role of CYP1B1 in PAH-DNA Adduct Formation and Breast Cancer Risk

    National Research Council Canada - National Science Library

    Goth-Goldstein, Regine

    2002-01-01

    ...) to reactive intermediates appears to be the cytochrome P45O enzyme CYPlB1. High CYPlB1 enzyme levels may result in increased formation of PAH-DNA adducts in breast tissue and lead to subsequent development of breast cancer...

  8. 26 CFR 31.3302(b)-1 - Additional credit against tax.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 15 2010-04-01 2010-04-01 false Additional credit against tax. 31.3302(b)-1...) EMPLOYMENT TAXES AND COLLECTION OF INCOME TAX AT SOURCE EMPLOYMENT TAXES AND COLLECTION OF INCOME TAX AT SOURCE Federal Unemployment Tax Act (Chapter 23, Internal Revenue Code of 1954) § 31.3302(b)-1 Additional...

  9. Intestinal bile salt absorption in Atp8b1 deficient mice

    NARCIS (Netherlands)

    Groen, Annemiek; Kunne, Cindy; Paulusma, Coen C.; Kramer, Werner; Agellon, Luis B.; Bull, Laura N.; Elferink, Ronald P. J. Oude

    2007-01-01

    BACKGROUND/AIMS: Mutations in the ATP8B1 gene can cause Progressive Familial Intrahepatic Cholestasis type 1. We have previously reported that Atp8b1(G308V/G308V) mice, a model for PFIC1, have slightly, but significantly, higher baseline serum bile salt (BS) concentrations compared to wt mice. Upon

  10. A subset of AID-dependent B-1a cells initiates hypersensitivity and pneumococcal pneumonia resistance

    NARCIS (Netherlands)

    Askenase, Phillip W; Bryniarski, Krzysztof; Paliwal, Vipin; Redegeld, Frank; Groot Kormelink, Thomas; Kerfoot, Steven; Hutchinson, Andrew T; van Loveren, Henk; Campos, Regis; Itakura, Atsuko; Majewska-Szczepanik, Monika; Yamamoto, Natsuo; Nazimek, Katarzyn; Szczepanik, Marian; Ptak, Wold

    2015-01-01

    We propose that there is a special B-1a B cell subset ("sB-1a" cells) that mediates linked processes very early after immunization to initiate cutaneous contact sensitivity (CS), delayed-type hypersensitivity (DTH), and immune resistance to pneumococcal pneumonia. Our published data indicate that in

  11. A lattice QCD calculation of the transverse decay constant of the b1(1235) meson

    International Nuclear Information System (INIS)

    Jansen, K.; McNeile, C.; Michael, C.; Urbach, C.

    2009-10-01

    We review various B meson decays that require knowledge of the transverse decay constant of the b 1 (1235) meson. We report on an exploratory lattice QCD calculation of the transverse decay constant of the b 1 meson. The lattice QCD calculations used unquenched gauge configurations, at two lattice spacings, generated with two flavours of sea quarks. The twisted mass formalism is used. (orig.)

  12. 26 CFR 1.802(b)-1 - Tax on life insurance companies.

    Science.gov (United States)

    2010-04-01

    .... For the definition of the term “1954 life insurance company taxable income”, see § 1.805-1. (b) The... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Tax on life insurance companies. 1.802(b)-1...) INCOME TAX (CONTINUED) INCOME TAXES Life Insurance Companies § 1.802(b)-1 Tax on life insurance companies...

  13. Production of milk-clotting enzyme by Bacillus subtilis B1 from wheat ...

    African Journals Online (AJOL)

    Three strains, Bacillus subtilis B1, B. subtilis B18 and Bacillus thuringiensis B12, were screened from wheat bran to produce milk-clotting enzyme. Among them, B. subtilis B1 exhibited considerable milkclotting activity with low proteolytic activity. After response surface methodology optimization, milkclotting activity was ...

  14. 26 CFR 1.6050B-1 - Information returns by person making unemployment compensation payments.

    Science.gov (United States)

    2010-04-01

    ... unemployment compensation payments. 1.6050B-1 Section 1.6050B-1 Internal Revenue INTERNAL REVENUE SERVICE... Information returns by person making unemployment compensation payments. For taxable years beginning after December 31, 1978, every person who makes payments of unemployment compensation (as defined in section 85...

  15. Biological reduction of aflatoxin B1 in wheat flour using yeast Saccharomyces cerevisiae

    Directory of Open Access Journals (Sweden)

    Mahsa Azimi

    2015-09-01

    Full Text Available Background: The safest way to reduce mycotoxins in contaminated foods with it it is using certain strains of various microbes. Aim of this study is biological reduction of aflatoxin B1 by the yeast Saccharomyces cerevisiae (S. cerevisiae in wheat flour samples that were collected in Chaloos city (Mazandaran province, North Iran. Material and Methods: Aflatoxin B1 contamination was measured by ELISA method in 22 samples of wheat flour. Then S. cerevisiae was added to primary wheat flour. After 48 h aflatoxin B1 contamination in samples were measured by ELISA, again. For statistical analysis, the statistical software SPSS18 and t-test was used. Results: all 22 samples of wheat flour were contaminated with aflatoxin B1. The minimum and maximum levels of aflatoxin B1 in primary wheat flour were 1.1 and 10.6 ppb, respectively. S. cerevisiae reduced the amount of aflatoxin B1 in wheat flour samples. Minimum and maximum amount of aflatoxin contamination after adding S. cerevisiae was 0.5 and 9.1 ppb, respectively. Conclusion: Inhibitory effect of S. cerevisiae and aflatoxin B1 reduction in wheat flour was proved in this study. Therefore we can be hoped that yeast S. cerevisiae as neutralizing agents can be used in flour for this toxin. Consumption of products that contaminated with aflatoxin B1 endangers consumers' health, thus reducing of this toxin in the food should be considered.

  16. Multiphoton-induced fluorescence and ionization of B1μ+ carbon monoxide

    International Nuclear Information System (INIS)

    Loge, G.W.; Tiee, J.J.; Wampler, F.B.

    1983-01-01

    The B 1 μ + state of carbon monoxide was created by a two-photon absorption of 230-nm laser radiation. The B 1 μ + state was identified by the positions of the 0-0 absorption band Q-branch and the vibrational bands of B 1 μ + → A 1 PI fluorescence occurring in the 525-nm region. Collision-induced b 3 μ + → a 3 PI emission in the 350-nm region was also observed. The lifetimes of both the B 1 μ + state and the b 3 μ + state were measured as well as the self-quenching rate constants and the B 1 μ + quenching rate by N 2 . In addition to the two-photon-induced fluorescence of B 1 μ + carbon monoxide, a three-photon ionization was observed. Laser power-dependence studies indicated that ionization of the B 1 μ + state is readily saturated, implying that most B 1 μ + state CO is ionized rather than fluorescing. Measurement of the polarization ratio for circularly and linearly polarized excitation of fluorescence and ionization suggests that another three-photon process is occurring and occurs more efficiently for linear polarization

  17. 26 CFR 41.4482(b)-1 - Definition of taxable gross weight.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 16 2010-04-01 2010-04-01 true Definition of taxable gross weight. 41.4482(b)-1... Motor Vehicles § 41.4482(b)-1 Definition of taxable gross weight. (a) Actual unloaded weight—(1) In... (such as air conditioning equipment and sanitation facilities, etc.); and a full complement of...

  18. Literatuuronderzoek naar HPLC-methoden voor de bepaling van vitamine B1 en B2

    NARCIS (Netherlands)

    Hollman, P.

    1986-01-01

    Analyses van vitamine B1 en B2 worden door de afdeling Additieven/Micronutrienten veelvuldig uitgevoerd in zeer diverse produkten. Dit stelt hoge eisen aan de selektiviteit van de gebruikte methoden. Onlangs werd dan ook HPLC geïntroduceerd voor de analyse van vitamine B1 en B2. Op basis van een

  19. UNC93B1 mediates host resistance to infection with Toxoplasma gondii.

    Directory of Open Access Journals (Sweden)

    Mariane B Melo

    2010-08-01

    Full Text Available UNC93B1 associates with Toll-Like Receptor (TLR 3, TLR7 and TLR9, mediating their translocation from the endoplasmic reticulum to the endolysosome, hence allowing proper activation by nucleic acid ligands. We found that the triple deficient '3d' mice, which lack functional UNC93B1, are hyper-susceptible to infection with Toxoplasma gondii. We established that while mounting a normal systemic pro-inflammatory response, i.e. producing abundant MCP-1, IL-6, TNFα and IFNγ, the 3d mice were unable to control parasite replication. Nevertheless, infection of reciprocal bone marrow chimeras between wild-type and 3d mice with T. gondii demonstrated a primary role of hemopoietic cell lineages in the enhanced susceptibility of UNC93B1 mutant mice. The protective role mediated by UNC93B1 to T. gondii infection was associated with impaired IL-12 responses and delayed IFNγ by spleen cells. Notably, in macrophages infected with T. gondii, UNC93B1 accumulates on the parasitophorous vacuole. Furthermore, upon in vitro infection the rate of tachyzoite replication was enhanced in non-activated macrophages carrying mutant UNC93B1 as compared to wild type gene. Strikingly, the role of UNC93B1 on intracellular parasite growth appears to be independent of TLR function. Altogether, our results reveal a critical role for UNC93B1 on induction of IL-12/IFNγ production as well as autonomous control of Toxoplasma replication by macrophages.

  20. Revaluasi Inventory dengan Menu Standard pada SAP-B1 Sesuaikah dengan IFRS

    Directory of Open Access Journals (Sweden)

    Eka Novianti

    2011-05-01

    Full Text Available Some available standard modules in SAP-B1 can be adapted for changes in accounting rules and regulations. One is the Inventory Revaluation menu of Inventory module. Users can use the menu to make Inventory value adjustments of value in SAP-B1, to be consistent with the values that must be presented in the financial statements. Based on the flexibility provided by SAP-B1menu, should not be too difficult for users of SAP-B1 to manage accounting transactions based on accounting rules that apply today, IFRS. IFRS requirements on inventory adjustment transactions with a value of SAP-B1 could be done more easily.

  1. Sulfolobus Replication Factor C stimulates the activity of DNA Polymerase B1

    DEFF Research Database (Denmark)

    Xing, Xuanxuan; Zhang, Likui; Guo, Li

    2014-01-01

    Replication factor C (RFC) is known to function in loading proliferating cell nuclear antigen (PCNA) onto primed DNA, allowing PCNA to tether DNA polymerase for highly processive DNA synthesis in eukaryotic and archaeal replication. In this report, we show that an RFC complex from...... the hyperthermophilic archaea of the genus Sulfolobus physically interacts with DNA polymerase B1 (PolB1) and enhances both the polymerase and 3'-5' exonuclease activities of PolB1 in an ATP-independent manner. Stimulation of the PolB1 activity by RFC is independent of the ability of RFC to bind DNA but is consistent...... with the ability of RFC to facilitate DNA binding by PolB1 through protein-protein interaction. These results suggest that Sulfolobus RFC may play a role in recruiting DNA polymerase for efficient primer extension, in addition to clamp loading, during DNA replication....

  2. Support interoperability and reusability of emerging forms of assessment: Some issues on integrating IMS LD with IMS QTI

    NARCIS (Netherlands)

    Miao, Yongwu; Boon, Jo; Van der Klink, Marcel; Sloep, Peter; Koper, Rob

    2009-01-01

    Miao, Y., Boon, J., Van der Klink, M., Sloep, P. B., & Koper, R. (2011). Support interoperability and reusability of emerging forms of assessment: Some issues on integrating IMS LD with IMS QTI. In F. Lazarinis, S. Green, & E. Pearson (Eds.), E-Learning Standards and Interoperability: Frameworks

  3. Antarctic observations available for IMS correlative analyses

    International Nuclear Information System (INIS)

    Rycroft, M.J.

    1982-01-01

    A review is provided of the wide-ranging observational programs of 25 stations operating on and around the continent of Antarctica during the International Magnetospheric Study (IMS). Attention is given to observations of geomagnetism, short period fluctuations of the earth's electromagnetic field, observations of the ionosphere and of whistler mode signals, observational programs in ionospheric and magnetospheric physics, upper atmosphere physics observations, details of magnetospheric programs conducted at Kerguelen, H-component magnetograms, magnetic field line oscillations, dynamic spectra of whistlers, and the variation of plasmapause position derived from recorded whistlers. The considered studies suggest that, in principle, if the level of magnetic activity is known, predictions can be made concerning the time at which the trough occurs, and the shape and the movement of the main trough

  4. FUNKTIONSLOGIK TERRORISTISCHER PROPAGANDA IM BEWEGTEN BILD

    Directory of Open Access Journals (Sweden)

    Stefan Christoph

    2015-09-01

    Full Text Available Die Medienfront ist heute ein wichtiger Kriegsschauplatz, den viele neben Land, Wasser und Luft für gleich wichtig halten. Terroristen fühlen sich in sozialen Medien wohler als man denken könnte. Im Netz können sie die asymmetrische Kräfteverteilung überwinden, die sie in der offenen Feldschlacht unterlegen sein ließe. Terroristen machen sich das Bild zur Waffe und produzieren erst durch die Positionierung an der Medienfront Sinn in ihren Taten. Ohne ein Bekennerschreiben, ein Abschiedsvideo des Attentäters oder ein letztes Posting im sozialen Netzwerk wäre ein Bombenanschlag nichts als ein Kapitalverbrechen. Durch die terroristische Kommunikationsstrategie wird das Verbrechen erst zum terroristischen Akt. Das ist die theoretische Grundannahme dieses Aufsatzes. Ohne Medienberichterstattung könnten terroristische Organisationen nicht existieren. Durch die Entwicklung des Web 2.0 und insbesondere sozialer Medien kommen terroristische Organisationen heutzutage aber ganz ohne das Verschicken von Videotapes an Fernsehsender aus. Durch YouTube und andere Kanäle, können sie die Adressaten ihrer Botschaften direkt und ohne zwischengeschaltete Journalistinnen und Journalisten erreichen. So ist es nicht erstaunlich, dass terroristische Organisationen sich eine gewisse Expertise im Umgang mit sozialen Medien zugelegt haben. Al-Qaida hat seine eigene Medienabteilung, auch die kolumbianischen FARC arbeiten sehr professionell und welchen Einfluss der Islamische Staat im Internet ausübt ist inzwischen bereits Gegenstand einiger Reportagen und Untersuchungen geworden. Die diesem Aufsatz zugrundeliegende Arbeit hat sich mit neun verschiedenen Videos aus dem Umfeld von al-Qaida, der IRA und der FARC beschäftigt und versucht, aus dem analytischen Vergleich dieser Videos Rückschlüsse über terroristische Medienmacher und die terroristischen Organisationen selbst zu ziehen. Wie schnell und suggestiv die Videos arbeiten, ist etwa davon abh

  5. Cyp26b1 within the growth plate regulates bone growth in juvenile mice

    International Nuclear Information System (INIS)

    Minegishi, Yoshiki; Sakai, Yasuo; Yahara, Yasuhito; Akiyama, Haruhiko; Yoshikawa, Hideki; Hosokawa, Ko; Tsumaki, Noriyuki

    2014-01-01

    Highlights: • Retinoic acid and Cyp26b1 were oppositely localized in growth plate cartilage. • Cyp26b1 deletion in chondrocytes decreased bone growth in juvenile mice. • Cyp26b1 deletion reduced chondrocyte proliferation and growth plate height. • Vitamin A-depletion partially reversed growth plate abnormalities caused by Cyp26b1 deficiency. • Cyp26b1 regulates bone growth by controlling chondrocyte proliferation. - Abstract: Retinoic acid (RA) is an active metabolite of vitamin A and plays important roles in embryonic development. CYP26 enzymes degrade RA and have specific expression patterns that produce a RA gradient, which regulates the patterning of various structures in the embryo. However, it has not been addressed whether a RA gradient also exists and functions in organs after birth. We found localized RA activities in the diaphyseal portion of the growth plate cartilage were associated with the specific expression of Cyp26b1 in the epiphyseal portion in juvenile mice. To disturb the distribution of RA, we generated mice lacking Cyp26b1 specifically in chondrocytes (Cyp26b1 Δchon cKO). These mice showed reduced skeletal growth in the juvenile stage. Additionally, their growth plate cartilage showed decreased proliferation rates of proliferative chondrocytes, which was associated with a reduced height in the zone of proliferative chondrocytes, and closed focally by four weeks of age, while wild-type mouse growth plates never closed. Feeding the Cyp26b1 cKO mice a vitamin A-deficient diet partially reversed these abnormalities of the growth plate cartilage. These results collectively suggest that Cyp26b1 in the growth plate regulates the proliferation rates of chondrocytes and is responsible for the normal function of the growth plate and growing bones in juvenile mice, probably by limiting the RA distribution in the growth plate proliferating zone

  6. Cyp26b1 within the growth plate regulates bone growth in juvenile mice

    Energy Technology Data Exchange (ETDEWEB)

    Minegishi, Yoshiki [Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Department of Plastic and Reconstructive Surgery, University of Fukui Hospital, 23-3 Matsuokashimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui 910-1193 (Japan); Department of Plastic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871 (Japan); Sakai, Yasuo [Department of Plastic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871 (Japan); Department of Plastic Surgery, Bellland General Hospital, 500-3 Higashiyama Naka-ku, Sakai, Osaka 599-8247 (Japan); Yahara, Yasuhito [Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Akiyama, Haruhiko [Department of Orthopaedic Surgery, Gifu University Graduate School of Medicine, 1-1 Yanagito, Gifu 501-1194 (Japan); Yoshikawa, Hideki [Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871 (Japan); Hosokawa, Ko [Department of Plastic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871 (Japan); Tsumaki, Noriyuki, E-mail: ntsumaki@cira.kyoto-u.ac.jp [Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan); Japan Science and Technology Agency, CREST, Tokyo 102-0075 (Japan)

    2014-11-07

    Highlights: • Retinoic acid and Cyp26b1 were oppositely localized in growth plate cartilage. • Cyp26b1 deletion in chondrocytes decreased bone growth in juvenile mice. • Cyp26b1 deletion reduced chondrocyte proliferation and growth plate height. • Vitamin A-depletion partially reversed growth plate abnormalities caused by Cyp26b1 deficiency. • Cyp26b1 regulates bone growth by controlling chondrocyte proliferation. - Abstract: Retinoic acid (RA) is an active metabolite of vitamin A and plays important roles in embryonic development. CYP26 enzymes degrade RA and have specific expression patterns that produce a RA gradient, which regulates the patterning of various structures in the embryo. However, it has not been addressed whether a RA gradient also exists and functions in organs after birth. We found localized RA activities in the diaphyseal portion of the growth plate cartilage were associated with the specific expression of Cyp26b1 in the epiphyseal portion in juvenile mice. To disturb the distribution of RA, we generated mice lacking Cyp26b1 specifically in chondrocytes (Cyp26b1{sup Δchon} cKO). These mice showed reduced skeletal growth in the juvenile stage. Additionally, their growth plate cartilage showed decreased proliferation rates of proliferative chondrocytes, which was associated with a reduced height in the zone of proliferative chondrocytes, and closed focally by four weeks of age, while wild-type mouse growth plates never closed. Feeding the Cyp26b1 cKO mice a vitamin A-deficient diet partially reversed these abnormalities of the growth plate cartilage. These results collectively suggest that Cyp26b1 in the growth plate regulates the proliferation rates of chondrocytes and is responsible for the normal function of the growth plate and growing bones in juvenile mice, probably by limiting the RA distribution in the growth plate proliferating zone.

  7. OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation

    Directory of Open Access Journals (Sweden)

    Lu Liu

    2017-01-01

    Full Text Available Introduction. Infection and apoptosis are combined triggers for inflammation in dental tissues. Octamer-binding transcription factor 4-B1 (OCT4B1, a novel spliced variant of OCT4 family, could respond to the cellular stress and possess antiapoptotic property. However, its specific role in dental pulpitis remains unknown. Methods. To investigate the effect of OCT4B1 on inflammation of dental pulp cells (DPCs, its expression in inflamed dental pulp tissues and DPCs was examined by in situ hybridization, real-time PCR, and FISH assay. OCT4B1 overexpressed DPCs model was established, confirmed by western blot and immunofluorescence staining, and then stimulated with Lipopolysaccharide (LPS. Apoptotic rate was determined by Hoechst/PI staining and FACS. Cell survival rate was calculated by CCK8 assay. Results. In situ hybridization, real-time PCR, and FISH assay revealed that OCT4B1 was extensively expressed in inflamed dental pulp tissues and DPCs with LPS stimulation. Western blot and immunofluorescence staining showed the expression of OCT4B1 and OCT4B increased after OCT4B1 transfection. Hoechst/PI staining and FACS demonstrated that less red/blue fluorescence was detected and apoptotic percentage decreased (3.45% after transfection. CCK8 demonstrated that the survival rate of pCDH-OCT4B1-flag cells increased. Conclusions. OCT4B1 plays an essential role in inflammation and apoptosis of DPCs. OCT4B might operate synergistically with OCT4B1 to reduce apoptosis.

  8. Key role for spinal dorsal horn microglial kinin B1 receptor in early diabetic pain neuropathy

    Directory of Open Access Journals (Sweden)

    Couture Réjean

    2010-06-01

    Full Text Available Abstract Background The pro-nociceptive kinin B1 receptor (B1R is upregulated on sensory C-fibres, astrocytes and microglia in the spinal cord of streptozotocin (STZ-diabetic rat. This study aims at defining the role of microglial kinin B1R in diabetic pain neuropathy. Methods Sprague-Dawley rats were made diabetic with STZ (65 mg/kg, i.p., and 4 days later, two specific inhibitors of microglial cells (fluorocitrate, 1 nmol, i.t.; minocycline, 10 mg/kg, i.p. were administered to assess the impact on thermal hyperalgesia, allodynia and mRNA expression (qRT-PCR of B1R and pro-inflammatory markers. Spinal B1R binding sites ((125I-HPP-desArg10-Hoe 140 were also measured by quantitative autoradiography. Inhibition of microglia was confirmed by confocal microscopy with the specific marker Iba-1. Effects of intrathecal and/or systemic administration of B1R agonist (des-Arg9-BK and antagonists (SSR240612 and R-715 were measured on neuropathic pain manifestations. Results STZ-diabetic rats displayed significant tactile and cold allodynia compared with control rats. Intrathecal or peripheral blockade of B1R or inhibition of microglia reversed time-dependently tactile and cold allodynia in diabetic rats without affecting basal values in control rats. Microglia inhibition also abolished thermal hyperalgesia and the enhanced allodynia induced by intrathecal des-Arg9-BK without affecting hyperglycemia in STZ rats. The enhanced mRNA expression (B1R, IL-1β, TNF-α, TRPV1 and Iba-1 immunoreactivity in the STZ spinal cord were normalized by fluorocitrate or minocycline, yet B1R binding sites were reduced by 38%. Conclusion The upregulation of kinin B1R in spinal dorsal horn microglia by pro-inflammatory cytokines is proposed as a crucial mechanism in early pain neuropathy in STZ-diabetic rats.

  9. SF3B1-initiating mutations in MDS-RSs target lymphomyeloid hematopoietic stem cells.

    Science.gov (United States)

    Mortera-Blanco, Teresa; Dimitriou, Marios; Woll, Petter S; Karimi, Mohsen; Elvarsdottir, Edda; Conte, Simona; Tobiasson, Magnus; Jansson, Monika; Douagi, Iyadh; Moarii, Matahi; Saft, Leonie; Papaemmanuil, Elli; Jacobsen, Sten Eirik W; Hellström-Lindberg, Eva

    2017-08-17

    Mutations in the RNA splicing gene SF3B1 are found in >80% of patients with myelodysplastic syndrome with ring sideroblasts (MDS-RS). We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. Screening for recurrently mutated genes in the mononuclear cell fraction revealed mutations in SF3B1 in 39 of 40 cases (97.5%), combined with TET2 and DNMT3A in 11 (28%) and 6 (15%) patients, respectively. All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. While in agreement with previous studies, little or no evidence for clonal ( SF3B1 mutation) involvement could be found in mature B cells, consistent involvement at the pro-B-cell progenitor stage was established, providing definitive evidence for SF3B1 mutations targeting lymphomyeloid HSCs and compatible with mutated SF3B1 negatively affecting lymphoid development. Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. Upon transplantation into immune-deficient mice, SF3B1 mutated MDS-RS HSCs differentiated into characteristic ring sideroblasts, the hallmark of MDS-RS. Our findings provide evidence of a multipotent lymphomyeloid HSC origin of SF3B1 mutations in MDS-RS patients and provide a novel in vivo platform for mechanistically and therapeutically exploring SF3B1 mutated MDS-RS. © 2017 by The American Society of Hematology.

  10. A study on the possibility of production of aflatoxin B1 in saffron

    Directory of Open Access Journals (Sweden)

    Abbas Mohammadi

    2017-06-01

    Full Text Available Saffron is the most important medicinal plants in the Khorasan Razavi and South Khorasan provinces, Iran. Aspergillus species can infect saffron tissues during harvesting, storage and transportation. Aflatoxin B1 is one of the important carcinogenic mycotoxin which is produced by Aspergillus species in saffron tissues. This study was carried out in order to investigate the production of aflatoxin B1 in saffron tissues from farm to food by TLC chromatography during the year 2015. Wet and dry saffron tissues and rice grains (with and without saffron extract were inoculated with Aspergillus flavus spore suspensions. Production of Aflatoxin B1 in inoculated tissues was investigated by the TLC chromatography method. The results showed that the wet leaves and rice grains were infected with Aspergillus species very quickly. However, this process was very slow in dry tissues. Aflatoxin B1 was detected in all of the tested samples. The amounts of Aflatoxin B1 in the wet saffron tissues and rice grains were more than those found in dry tissues and saffron rice, respectively. The amount of Aflatoxin B1 had a direct correlation with moisture in the environment and the tissues. The contamination of the tissues and production of AflatoxinB1 were increased with increasing the amount of moisture. The results showed that the packaging of saffron before complete drying of its tissue or storing it in conditions with high humidity can increase the risk of infection with the Aspergillus species and production of Aflatoxin B1 in them. Based on our data, saffron can reduce Aspergillus infection and aflatoxin B1 production but not inhibit it. Saffron extract reduces Aspergillus infection and Aflatoxin B1 production in food and grains.

  11. Cinnamtannin B-1 Promotes Migration of Mesenchymal Stem Cells and Accelerates Wound Healing in Mice.

    Directory of Open Access Journals (Sweden)

    Kosuke Fujita

    Full Text Available Substances that enhance the migration of mesenchymal stem cells to damaged sites have the potential to improve the effectiveness of tissue repair. We previously found that ethanol extracts of Mallotus philippinensis bark promoted migration of mesenchymal stem cells and improved wound healing in a mouse model. We also demonstrated that bark extracts contain cinnamtannin B-1, a flavonoid with in vitro migratory activity against mesenchymal stem cells. However, the in vivo effects of cinnamtannin B-1 on the migration of mesenchymal stem cells and underlying mechanism of this action remain unknown. Therefore, we examined the effects of cinnamtannin B-1 on in vivo migration of mesenchymal stem cells and wound healing in mice. In addition, we characterized cinnamtannin B-1-induced migration of mesenchymal stem cells pharmacologically and structurally. The mobilization of endogenous mesenchymal stem cells into the blood circulation was enhanced in cinnamtannin B-1-treated mice as shown by flow cytometric analysis of peripheral blood cells. Whole animal imaging analysis using luciferase-expressing mesenchymal stem cells as a tracer revealed that cinnamtannin B-1 increased the homing of mesenchymal stem cells to wounds and accelerated healing in a diabetic mouse model. Additionally, the cinnamtannin B-1-induced migration of mesenchymal stem cells was pharmacologically susceptible to inhibitors of phosphatidylinositol 3-kinase, phospholipase C, lipoxygenase, and purines. Furthermore, biflavonoids with similar structural features to cinnamtannin B-1 also augmented the migration of mesenchymal stem cells by similar pharmacological mechanisms. These results demonstrate that cinnamtannin B-1 promoted mesenchymal stem cell migration in vivo and improved wound healing in mice. Furthermore, the results reveal that cinnamtannin B-1-induced migration of mesenchymal stem cells may be mediated by specific signaling pathways, and the flavonoid skeleton may be

  12. Key role for spinal dorsal horn microglial kinin B1 receptor in early diabetic pain neuropathy.

    Science.gov (United States)

    Talbot, Sébastien; Chahmi, Emna; Dias, Jenny Pena; Couture, Réjean

    2010-06-29

    The pro-nociceptive kinin B1 receptor (B1R) is upregulated on sensory C-fibres, astrocytes and microglia in the spinal cord of streptozotocin (STZ)-diabetic rat. This study aims at defining the role of microglial kinin B1R in diabetic pain neuropathy. Sprague-Dawley rats were made diabetic with STZ (65 mg/kg, i.p.), and 4 days later, two specific inhibitors of microglial cells (fluorocitrate, 1 nmol, i.t.; minocycline, 10 mg/kg, i.p.) were administered to assess the impact on thermal hyperalgesia, allodynia and mRNA expression (qRT-PCR) of B1R and pro-inflammatory markers. Spinal B1R binding sites ((125I)-HPP-desArg10-Hoe 140) were also measured by quantitative autoradiography. Inhibition of microglia was confirmed by confocal microscopy with the specific marker Iba-1. Effects of intrathecal and/or systemic administration of B1R agonist (des-Arg9-BK) and antagonists (SSR240612 and R-715) were measured on neuropathic pain manifestations. STZ-diabetic rats displayed significant tactile and cold allodynia compared with control rats. Intrathecal or peripheral blockade of B1R or inhibition of microglia reversed time-dependently tactile and cold allodynia in diabetic rats without affecting basal values in control rats. Microglia inhibition also abolished thermal hyperalgesia and the enhanced allodynia induced by intrathecal des-Arg9-BK without affecting hyperglycemia in STZ rats. The enhanced mRNA expression (B1R, IL-1beta, TNF-alpha, TRPV1) and Iba-1 immunoreactivity in the STZ spinal cord were normalized by fluorocitrate or minocycline, yet B1R binding sites were reduced by 38%. The upregulation of kinin B1R in spinal dorsal horn microglia by pro-inflammatory cytokines is proposed as a crucial mechanism in early pain neuropathy in STZ-diabetic rats.

  13. Asbestos in drinking water; Asbest im Trinkwasser

    Energy Technology Data Exchange (ETDEWEB)

    Spurny, K.R.

    1992-12-01

    Measurements and analysis of more than 100 samples of tap-water, originated from different countries of the Federal Republik of Germany, have been performed by means of the standardized TEM-procedure (ISO). The results have shown that the drinking water is contaminated with fine fibers, with chrysotile and amphibole asbestos. The majority of investigated samples contained less than 10{sup 6} fibers/liter, and the fibers were thin and shorter than 5 {mu}m. Nevertheless, in some tap-water samples the asbestos fiber concentrations were higher than 10{sup 6} fibers/liter and/or the content of long fibers (longer than 5 {mu}m) was relatively high. It is recommended tapwater with asbestos fiber concentrations over 10{sup 6} fibers/liter and/or with greater content of long fibers should not be used for cooking or drinking unless filtered. (orig.) [Deutsch] Mehr als 100 Trinkwasser-Proben aus verschiedenen Bundeslaendern wurden untersucht und analysiert bei Anwendung eines standardisierten TEM-Verfahrens (ISO). Die Ergebnisse zeigten, dass Trinkwasser (alte Bundeslaender) mit feinen Asbestfasern, Chrysotil und Amphibolen, mehr oder weniger kontaminiert ist. In der Mehrheit der untersuchten Proben lagen die Asbestkonzentrationen im Bereich weniger als 10{sup 6} Fasern/Liter und die gemessenen Fasern waren duenn und kuerzer als 5 {mu}m. Nichtsdestoweniger, in einigen Wasserproben wurden Asbestfaser-Kontaminationen im Bereich ueber 10{sup 6} Fasern/Liter ermittelt. Diese Wasserproben enthielten auch hoeheren Anteil an langen Fasern. Es wird empfohlen, Wasser mit Asbestfaserkonzentrationen ueber 10{sup 6} Fasern/Liter oder mit einem hoeheren Anteil an langen Asbestfasern nicht ohne weitere Behandlung (Filtration) zu trinken und nicht zum Kochen zu verwenden. (orig.)

  14. Die Pluralbildung im Deutschen - ein Versuch im Rahmen der Optimalitätstheorie

    Directory of Open Access Journals (Sweden)

    Wegener, Heide

    1999-01-01

    Full Text Available Die Pluralbildung im Deutschen ist nicht chaotisch, aber komplex. Unabhängig davon, ob man für die Pluralbildung Regeln, Schemata oder die Wortstruktur als Erklärungsmodell benutzt, bleibt die Tatsache einer beachtlichen Zahl miteinander konkurrierender Formen zu erklären. Dies gilt schon deshalb, weil die Variation unter den Pluralformen wohl mit dazu beiträgt, dass trotz der Arbeiten von Mugdan 1977 und Köpcke 1987, 1993, der klaren Darstellung des Pluralsystems in vielen Grammatiken, z.B. Eisenberg 1986, 1989, 1998 noch immer bisweilen die Meinung besteht, die Pluralbildung im Deutschen sei arbiträr.Es soll gezeigt werden, dass das Pluralsystem des Deutschen nach Beschränkungen aufgebaut ist, die morphologischer und silbenphonologischer Natur sind und sich im Rahmen der Optimalitätstheorie (OT beschreiben lassen. Die OT nimmt an, dass Wortformen universalen Beschränkungen (Constraints unterliegen, und dass diese prinzipiell verletzbar sind. Ich werde zunächst die für die Pluralbildung relevanten Beschränkungen definieren, dann an heimischen Pluralformen und -varianten ihr Zusammenwirken zeigen und schließlich den Assimilationsprozess von Fremdwörtern mit Hilfe dieser Beschränkungen und der Beschränkungshierarchien zu erklären versuchen. Daran werden schließlich Überlegungen zu Verarbeitung und Lernbarkeit der Pluralformen geknüpft.

  15. CYP1B1 Cytopathy: Uncommon Phenotype of a Homozygous CYP1B1 Deletion as Internal Corneal Ulcer of Von Hippel.

    Science.gov (United States)

    Oliva-Biénzobas, Valeria; Navas, Alejandro; C Astiazarán, Mirena; Chacón-Camacho, Oscar Francisco; A Bermúdez-Magner, Jose; Takane, Mariana; Graue-Hernández, Enrique; Zenteno, Juan Carlos

    2017-10-01

    To report a mutation of CYP1B1 in a newborn with a rare phenotype without the classic features of anterior segment dysgenesis or congenital glaucoma. The newborn presented with diffuse corneal edema and bilaterally elevated intraocular pressure (IOP). Ophthalmological examination, ultrasound, and ultrasound biomicroscopy were performed; congenital infections were ruled out. Genetic analysis was performed. The patient underwent penetrating keratoplasty and goniotomy in a single surgical time. The button was subjected to histopathological examination. The patient is the first child of young, healthy, consanguineous parents. Ophthalmological examination revealed visual acuity of light perception and increased IOP in both eyes. CYP1B1 gene analysis demonstrated homozygosity for a 1-bp deletion in exon 2 (c.830delT). IOP was normalized, and the corneal button was clear after surgical treatment. Histopathological analysis revealed loss of the Bowman membrane in the central cornea, fibrosis of the stroma, absence of endothelial cells, and loss of Descemet membrane centrally. We present an uncommon mutation and clinical description of CYP1B1. This report and further studies could provide us better understanding of the mutational spectrum of CYP1B1.

  16. Vascular Kinin B1 and B2 Receptors Determine Endothelial Dysfunction through Neuronal Nitric Oxide Synthase

    Directory of Open Access Journals (Sweden)

    Luciano Dos Santos A. Capettini

    2017-04-01

    Full Text Available B1- and B2-kinin receptors are G protein-coupled receptors that play an important role in the vascular function. Therefore, the present study was designed to evaluate the participation of kinin receptors in the acetylcholine (ACh-induced vascular relaxation, focusing on the protein-protein interaction involving kinin receptors with endothelial and neuronal nitric oxide synthases (eNOS and nNOS. Vascular reactivity, nitric oxide (NO· and reactive oxygen species (ROS generation, co-immunoprecipitation were assessed in thoracic aorta from male wild-type (WT, B1- (B1R−/−, B2- (B2R−/− knockout mice. Some vascular reactivity experiments were also performed in a double kinin receptors knockout mice (B1B2R−/−. For pharmacological studies, selective B1- and B2-kinin receptors antagonists, NOS inhibitors and superoxide dismutase (SOD mimetic were used. First, we show that B1- and B2-kinin receptors form heteromers with nNOS and eNOS in thoracic aorta. To investigate the functionality of these protein-protein interactions, we took advantage of pharmacological tools and knockout mice. Importantly, our results show that kinin receptors regulate ACh-induced relaxation via nNOS signaling in thoracic aorta with no changes in NO· donor-induced relaxation. Interestingly, B1B2R−/− presented similar level of vascular dysfunction as found in B1R−/− or B2R−/− mice. In accordance, aortic rings from B1R−/− or B2R−/− mice exhibit decreased NO· bioavailability and increased superoxide generation compared to WT mice, suggesting the involvement of excessive ROS generation in the endothelial dysfunction of B1R−/− and B2R−/− mice. Alongside, we show that impaired endothelial vasorelaxation induced by ACh in B1R−/− or B2R−/− mice was rescued by the SOD mimetic compound. Taken together, our findings show that B1- and B2-kinin receptors regulate the endothelium-dependent vasodilation of ACh through nNOS activity and indicate

  17. Laminar shear stress regulates endothelial kinin B1 receptor expression and function: potential implication in atherogenesis

    Science.gov (United States)

    Duchene, Johan; Cayla, Cécile; Vessillier, Sandrine; Scotland, Ramona; Yamashiro, Kazuo; Lecomte, Florence; Syed, Irfan; Vo, Phuong; Marrelli, Alessandra; Pitzalis, Costantino; Cipollone, Francesco; Schanstra, Joost; Bascands, Jean-Loup; Hobbs, Adrian J; Perretti, Mauro; Ahluwalia, Amrita

    2009-01-01

    OBJECTIVE The pro-inflammatory phenotype induced by low laminar shear stress (LSS) is implicated in atherogenesis. The kinin B1 receptor (B1R), known to be induced by inflammatory stimuli, exerts many pro-inflammatory effects including vasodilatation and leukocyte recruitment. We investigated whether low LSS is a stimulus for endothelial B1R expression and function. METHODS AND RESULTS Human and mouse atherosclerotic plaques expressed high level of B1R mRNA and protein. In addition, B1R expression was upregulated in the aortic arch (low LSS region) of ApoE-/- mice fed a high fat diet compared to vascular regions of high LSS and animals fed normal chow. Of interest, a greater expression of B1R was noticed in endothelial cells from regions of low LSS in aortic arch of ApoE-/- mice. B1R was also upregulated in human umbilical vein endothelial cells (HUVEC) exposed to low LSS (0-2dyn/cm2) compared to physiological LSS (6-10dyn/cm2): an effect similarly evident in murine vascular tissue perfused ex vivo. Functionally, B1R activation increased prostaglandin and CXCL5 expression in cells exposed to low, but not physiological, LSS. IL-1β and ox-LDL induced B1R expression and function in HUVECs, a response substantially enhanced under low LSS conditions and inhibited by blockade of NFκB activation. CONCLUSION Herein, we show that LSS is a major determinant of functional B1R expression in endothelium. Furthermore, whilst physiological high LSS is a powerful repressor of this inflammatory receptor, low LSS at sites of atheroma are associated with substantial upregulation, identifying this receptor as a potential therapeutic target. CONDENSED ABSTRACT Low laminar shear stress (LSS) underlies the pro-inflammatory processes in atherogenesis. Herein, we demonstrate that whilst physiological LSS represses inflammatory kinin B1 receptor (B1R) expression/function, low atherogenic LSS is associated with profound upregulation of both in atherosclerosis in both humans and animal

  18. Structure-function relationships of the Mycobacterium tuberculosis transcription factor WhiB1.

    Directory of Open Access Journals (Sweden)

    Laura J Smith

    Full Text Available Members of the WhiB-like (Wbl protein family possess iron-sulfur clusters and are implicated in the regulation of developmental processes in Actinomycetes. Mycobacterium tuberculosis possesses seven Wbl proteins. The [4Fe-4S] cluster of M. tuberculosis WhiB1 is relatively insensitive to O(2 but very sensitive to nitric oxide (NO. Nitric oxide nitrosylates the WhiB1 iron-sulfur cluster and promotes DNA-binding; the apo-forms of WhiB1 also bind DNA. However, the molecular requirements for iron-sulfur cluster acquisition and for DNA-binding by WhiB1 are poorly characterized.WhiB1 variants were created by site-directed mutagenesis and the abilities of the corresponding proteins to acquire an iron-sulfur cluster and/or bind to whiB1 promoter DNA were assessed. All four Cys residues (Cys9, 37, 40, and 46 in the N-terminal region of WhiB1 were required for incorporation of a [4Fe-4S] cluster, whereas a possible alternative cluster ligand Asp13 (by analogy with M. smegmatis WhiB2 was not. The C-terminal region of WhiB1 is predicted to house the DNA-binding domain of the protein consisting of a predicted β-turn ((58GVWGG(62 followed by two amino acid motifs ((72KRRN(75 and (78TKAR(81 that are conserved in WhiB1 proteins. Gly residues (Gly58, 61 and 62 in the β-turn and positively-charged residues (Lys72, Arg73, Arg74, Lys79 and Arg81 in the downstream conserved regions were required for binding of WhiB1 DNA.Site-directed mutagenesis of M. tuberculosis whiB1 and characterization of the corresponding proteins has been used to explore structure-function relationships of the NO-responsive transcription factor WhiB1. This showed that all four conserved Cys residues in the N-terminal region are required for incorporation of iron-sulfur clusters but not for DNA-binding. Analysis of variants with amino acid substitutions in the C-terminal region revealed the crucial roles played by a predicted β-turn and two conserved positively-charged motifs in facilitating

  19. Tensor-polarized structure function b1 in the standard convolution description of the deuteron

    Science.gov (United States)

    Cosyn, W.; Dong, Yu-Bing; Kumano, S.; Sargsian, M.

    2017-04-01

    Tensor-polarized structure functions of a spin-1 hadron are additional observables, which do not exist for the spin-1 /2 nucleon. They could probe novel aspects of the internal hadron structure. Twist-2 tensor-polarized structure functions are b1 and b2, and they are related by the Callan-Gross-like relation in the Bjorken scaling limit. In this work, we theoretically calculate b1 in the standard convolution description for the deuteron. Two different theoretical models, a basic convolution description and a virtual nucleon approximation, are used for calculating b1, and their results are compared with the HERMES measurement. We found large differences between our theoretical results and the data. Although there is still room to improve by considering higher-twist effects and in the experimental extraction of b1 from the spin asymmetry Az z, there is a possibility that the large differences require physics beyond the standard deuteron model for their interpretation. Future b1 studies could shed light on a new field of hadron physics. In particular, detailed experimental studies of b1 will start soon at the Thomas Jefferson National Accelerator Facility. In addition, there are possibilities to investigate tensor-polarized parton distribution functions and b1 at Fermi National Accelerator Laboratory and a future electron-ion collider. Therefore, further theoretical studies are needed for understanding the tensor structure of the spin-1 deuteron, including a new mechanism to explain the large differences between the current data and our theoretical results.

  20. SR-B1 Is a Silica Receptor that Mediates Canonical Inflammasome Activation

    Directory of Open Access Journals (Sweden)

    Misato Tsugita

    2017-01-01

    Full Text Available The inhalation of silica dust is associated with fibrosis and lung cancer, which are triggered by macrophage inflammatory responses; however, how macrophages recognize silica remains largely unknown. Here, we identify by functional expression cloning the class B scavenger receptor SR-B1 as a silica receptor. Through an extracellular α-helix, both mouse and human SR-B1 specifically recognized amorphous and crystalline silica, but not titanium dioxide nanoparticles, latex nanoparticles, or monosodium urate crystals, although all particles exhibited negative surface potentials. Genetic deletion of SR-B1 and masking of SR-B1 by monoclonal antibodies showed that SR-B1-mediated recognition of silica is associated with caspase-1-mediated inflammatory responses in mouse macrophages and human peripheral blood monocytes. Furthermore, SR-B1 was involved in silica-induced pulmonary inflammation in mice. These results indicate that SR-B1 is a silica receptor associated with canonical inflammasome activation.

  1. Spatiotemporal Expression Patterns and Antibody Reactivity of Taeniidae Endophilin B1

    Science.gov (United States)

    Ahn, Chun-Seob; Bae, Young-An; Kim, Seon-Hee; Kim, Jeong-Geun; Yu, Jae-Ran; Yang, Hyun-Jong; Eom, Keeseon S.; Wang, Hu; Kang, Insug; Yang, Yichao

    2016-01-01

    Larval Taeniidae, such as metacestodes of Taenia solium, Echinococcus granulosus, and Echinococcus multilocularis, produce chronic and fatal helminthic diseases. Proper identification of these zoonotic cestodiases is often challenging and is hampered in some clinical settings. Endophilin B1 plays critical roles in the maintenance of membrane contours and endocytosis. We isolated proteins homologous to endophilin B1 from T. solium, Taenia saginata, and Taenia asiatica. The three Taeniidae endophilin B1 proteins shared 92.9 to 96.6% sequence identity. They harbored a Bin1/amphiphysin/Rvs (BAR) domain and residues for a dimeric interface but lacked a SRC homology 3 (SH3) domain. Endophilin B1 showed a unique immunological profile and was abundantly expressed in the tegumental syncytium of Taeniidae metacestodes and adults. Bacterially expressed recombinant T. solium endophilin B1 (rTsMEndoB1) demonstrated a sensitivity of 79.7% (345/433 cases) for serodiagnosis of larval Taeniidae infections. The protein showed strong immune recognition patterns against sera from patients with chronic neurocysticercosis, cystic echinococcosis, or advanced-stage alveolar echinococcosis. Adult Taeniidae infections exhibited moderate degrees of positive antibody responses (65.7% [23/35 samples]). rTsMEndoB1 showed some cross-reactivity with sera from patients infected with Diphyllobothriidae (23.6% [25/106 samples]) but not with sera from patients with other parasitic diseases or normal controls. The specificity was 91.7% (256/301 samples). The positive and negative predictive values were 93.6% and 73.4%, respectively. Our results demonstrate that Taeniidae endophilin B1 may be involved in the control of membrane dynamics, thus contributing to shaping and maintaining the tegumental curvature. rTsMEndoB1 may be useful for large-scale screening, as well as for individual diagnosis and follow-up surveillance of Taeniidae infections. PMID:27487955

  2. Contribution of CYP1B1 mutations and founder effect to primary congenital glaucoma in Mexico.

    Science.gov (United States)

    Zenteno, Juan Carlos; Hernandez-Merino, Elena; Mejia-Lopez, Herlinda; Matías-Florentino, Margarita; Michel, Norma; Elizondo-Olascoaga, Celia; Korder-Ortega, Vincent; Casab-Rueda, Homero; Garcia-Ortiz, Jose Elias

    2008-01-01

    The frequency of primary congenital glaucoma (PCG)-causing CYP1B1 mutations varies importantly among distinct populations, ranging from 20% in Indonesians and Japanese to about 100% among the Saudi Arabians and Slovakian Gypsies. Thus, the molecular characterization of large groups of PCG from different ethnic backgrounds is important to establish the actual CYP1B1 contribution in specific populations. In this work, the molecular analysis of the CYP1B1 gene in a group of Mexican PCG patients is reported. Thirty unrelated Mexican patients fulfilling the clinical criteria for PCG were included. Two cases were familial and with proven consanguinity, originating from distinct regions of the country. Polymerase chain reaction amplification and direct automated sequencing of the CYP1B1 coding region was performed in each participating subject. An identical pathogenic CYP1B1 mutation was demonstrated in 2 unrelated PCG subjects. The mutation consisted of a homozygous G to A transition at nucleotide position 1505 in exon 3, which predicted a substitution of glutamic acid for lysine at residue 387 of the protein (E387K). In the remaining 28 PCG subjects, no deleterious mutations were identified. Both subjects with the E387K mutation shared a same haplotype for 5 CYP1B1 intragenic single nucleotide polymorphisms, indicating a common origin of the allele. Mexican patients with PCG are rarely (less than 10%) due to CYP1B1 mutations. Available data indicate that most of the non-Brazilian Latin American PCG patients investigated to date are not due to CYP1B1 defects. Populations with low incidence of CYP1B1 mutations are appropriate candidates for the identification of novel PCG-causing genes.

  3. Rhythmic control of activity and sleep by class B1 GPCRs.

    Science.gov (United States)

    Kunst, Michael; Tso, Matthew C F; Ghosh, D Dipon; Herzog, Erik D; Nitabach, Michael N

    2015-01-01

    Members of the class B1 family of G-protein coupled receptors (GPCRs) whose ligands are neuropeptides have been implicated in regulation of circadian rhythms and sleep in diverse metazoan clades. This review discusses the cellular and molecular mechanisms by which class B1 GPCRs, especially the mammalian VPAC2 receptor and its functional homologue PDFR in Drosophila and C. elegans, regulate arousal and daily rhythms of sleep and wake. There are remarkable parallels in the cellular and molecular roles played by class B1 intercellular signaling pathways in coordinating arousal and circadian timekeeping across multiple cells and tissues in these very different genetic model organisms.

  4. Use of gamma irradiation to prevent aflatoxin B1 production in smoked dried fish

    International Nuclear Information System (INIS)

    Ogbadu, G.H.

    1988-01-01

    Smoked dried fish bought from the Nigerian market was inoculated with spores of Aspergillus flavus (U.I. 81) and irradiated with doses of 0.625, 1.25, 2.50 and 5.00 kGy gamma irradiation. The effect on aflatoxin B 1 production on subsequent incubation for 8 days as stationary cultures was measured. The amount of aflatoxin B 1 produced was found to decrease with increased gamma irradiation dose levels. The non-irradiated control produced significantly (at 1% level) greater amounts of aflatoxin B 1 as compared to the treated cultures. (author)

  5. EphB1 as a Novel Drug Target to Combat Pain and Addiction

    Science.gov (United States)

    2016-09-01

    Award Number: W81XWH-14-1-0220 Project Title: EphB1 as a Novel Drug Target to Combat Pain and Addiction Principal Investigator Name: Mark...Pain and Addiction 5a. CONTRACT NUMBER EphB1 as a Novel Drug Target to Combat Pain and Addiction 5b. GRANT NUMBER W81XWH-14-1-0220 5c. PROGRAM...SUBJECT TERMS Chronic neuropathic pain, opioid addiction , synaptic plasticity, EphB1 receptor, ephrin-B2, NMDA receptor, drug discovery 16. SECURITY

  6. CYP1B1 Mutations in Individuals With Primary Congenital Glaucoma and Residing in Denmark

    DEFF Research Database (Denmark)

    Grønskov, Karen; Redó-Riveiro, Alba; Sandfeld, Lisbeth

    2016-01-01

    Primary congenital glaucoma (PCG OMIM 231300) can be caused by pathogenic sequence variations in cytochrome P450, subfamily 1, polypeptide 1 (CYP1B1). The purpose of this study was to investigate the contribution of sequence variations in CYP1B1 in a cohort of individuals with PCG residing...... mutations, 5 of which were novel. The frequency of CYP1B1 mutations in this cohort was comparable with other populations. We also detected an individual heterozygous for p.(Tyr81Asn) mutation, previously suggested to cause autosomal dominant primary open-angle glaucoma....

  7. Literatuuronderzoek naar HPLC-methoden voor de bepaling van vitamine B1 en B2

    OpenAIRE

    Hollman, P.

    1986-01-01

    Analyses van vitamine B1 en B2 worden door de afdeling Additieven/Micronutrienten veelvuldig uitgevoerd in zeer diverse produkten. Dit stelt hoge eisen aan de selektiviteit van de gebruikte methoden. Onlangs werd dan ook HPLC geïntroduceerd voor de analyse van vitamine B1 en B2. Op basis van een literatuuronderzoek werd toen gekozen voor een simultane bepaling van vitamine B1 en B2. Inmiddels is echter gebleken dat de enzymen die gebruikt worden voor de hydrolyse van de fosfaatesters niet mee...

  8. IMS Meteorological Data, Current Status and Improvement of Data Quality

    Science.gov (United States)

    Martysevich, Pavel; Marty, Julien; Polzer, Peter

    2015-04-01

    The objective of the poster is to present the efforts made by the Provisional Technical Secretariat (PTS) over the last 3 years to assess and improve the quality of the meteorological data recorded at International Monitoring System (IMS) infrasound stations. This includes the processing and assessment of the data recorded since 2000, the organization of Expert Group Meetings, comparison of the IMS and World Meteorological Organization requirements to the meteorological data and the list of actions taken/to be taken for improving the reliability and accuracy of IMS meteorological data.

  9. Das Surfgramm als grafische Darstellung von Surfwegen im Internet

    Directory of Open Access Journals (Sweden)

    Martina Schuegraf

    2007-12-01

    Full Text Available Das folgend vorgestellte Instrument zur Analyse und Darstellung von Surfwegen im Internet, die durch ihre Nutzerinnen und Nutzer vollzogen werden, kann und soll nicht als ein technisch komplexes und «objektive» Daten lieferndes Methodeninstrumentarium betrachtet werden. Es ist vielmehr eine Methode, um im Bereich der qualitativen (Internet-Forschung in einer strukturierenden und fassbaren Weise Handlungsformen im Internet sichtbar und auf diese Weise auswertbar zu machen. Diese Form der Auswertung geht über die reine Interpretation der Transkripttexte von Interviews hinaus, sollte aber damit trianguliert werden.

  10. Die Standardisierung des Marketing im internationalen E-Commerce

    OpenAIRE

    Fritz, Wolfgang; Dees, Heiko

    2005-01-01

    Die Standardisierung des Marketing ist seit den 1960er Jahren eine Schlüsselfrage bei der Internationalisierung von Unternehmen, zu der in den letzten Jahrzehnten zahlreiche Beiträge veröffentlicht worden sind. Allerdings fehlt es bisher an Studien, die dieser Frage im Rahmen des internationalen E-Commerce nachgehen. Die Autoren zeigen im vorliegenden Beitrag empirisch, dass die einzelnen Marketing-Instrumente und -Prozesse im internationalen B2C-E-Commerce in einem unterschiedlich hohen Maße...

  11. Onderzoek naar aflatoxine B1 in mengvoeders en grondstoffen : jaaroverzicht 1987

    NARCIS (Netherlands)

    Roos, A.H.; Tuinstra, L.G.M.T.; Huf, F.A.; Mazijk, van R.J.; Traag, W.A.; Trijp, van J.M.P.

    1988-01-01

    Het rapport geeft een overzicht van de resultaten van het onderzoek naar aflatoxine B1 volgens de HPLC screeningsmethode in rundveevoeders, grondnoten, varkensvoeder, een legpluimveevoeder, een kokos/soyapalmpitmengsel en een rnaismonster in 1987.

  12. Use of radiolabeled monoclonal anti-B1 antibody for B lymphocyte imaging in Rhesus monkeys

    International Nuclear Information System (INIS)

    Letvin, N.L.; Zalutsky, M.R.; Chalifoux, L.V.; Atkins, H.L.

    1987-01-01

    Imaging tissues rich in B lymphocytes in man using a radiolabeled monoclonal anti-B cell antibody would be extremely useful in the clinical staging of non-Hodgkins lymphomas. Studies were done in rhesus monkeys using radiolabeled monoclonal anti-B1 antibody to determine the feasibility of such an approach. Immunohistologic studies demonstrated that infused monoclonal anti-B1 binds in vivo with specificity to B cells in lymph nodes and spleen. The kinetics of clearance of 131 I-labeled anti-B1 were determined. The B lymphocyte-rich spleen could be readily visualized by gamma camera scanning without significant background and without the need for image intensification or blood background subtraction techniques. These data support the feasibility of using anti-B1 for staging B cell lymphomas in man. (author)

  13. The role of scavenger receptor B1 in infection with Mycobacterium tuberculosis in a murine model.

    Directory of Open Access Journals (Sweden)

    Georgia Schäfer

    2009-12-01

    Full Text Available The interaction between Mycobacterium tuberculosis (Mtb and host cells is complex and far from being understood. The role of the different receptor(s implicated in the recognition of Mtb in particular remains poorly defined, and those that have been found to have activity in vitro were subsequently shown to be redundant in vivo.To identify novel receptors involved in the recognition of Mtb, we screened a macrophage cDNA library and identified scavenger receptor B class 1 (SR-B1 as a receptor for mycobacteria. SR-B1 has been well-described as a lipoprotein receptor which mediates both the selective uptake of cholesteryl esters and the efflux of cholesterol, and has also recently been implicated in the recognition of other pathogens. We show here that mycobacteria can bind directly to SR-B1 on transfected cells, and that this interaction could be inhibited in the presence of a specific antibody to SR-B1, serum or LDL. We define a variety of macrophage populations, including alveolar macrophages, that express this receptor, however, no differences in the recognition and response to mycobacteria were observed in macrophages isolated from SR-B1(-/- or wild type mice in vitro. Moreover, when wild type and SR-B1(-/- animals were infected with a low dose of Mtb (100 CFU/mouse there were no alterations in survival, bacterial burdens, granuloma formation or cytokine production in the lung. However, significant reduction in the production of TNF, IFNgamma, and IL10 were observed in SR-B1(-/- mice following infection with a high dose of Mtb (1000 CFU/mouse, which marginally affected the size of inflammatory foci but did not influence bacterial burdens. Deficiency of SR-B1 also had no effect on resistance to disease under conditions of varying dietary cholesterol. We did observe, however, that the presence of high levels of cholesterol in the diet significantly enhanced the bacterial burdens in the lung, but this was independent of SR-B1.SR-B1 is involved in

  14. Cognitive and cerebrovascular improvements following kinin B1 receptor blockade in Alzheimer’s disease mice

    Science.gov (United States)

    2013-01-01

    Background Recent evidence suggests that the inducible kinin B1 receptor (B1R) contributes to pathogenic neuroinflammation induced by amyloid-beta (Aβ) peptide. The present study aims at identifying the cellular distribution and potentially detrimental role of B1R on cognitive and cerebrovascular functions in a mouse model of Alzheimer’s disease (AD). Methods Transgenic mice overexpressing a mutated form of the human amyloid precursor protein (APPSwe,Ind, line J20) were treated with a selective and brain penetrant B1R antagonist (SSR240612, 10 mg/kg/day for 5 or 10 weeks) or vehicle. The impact of B1R blockade was measured on i) spatial learning and memory performance in the Morris water maze, ii) cerebral blood flow (CBF) responses to sensory stimulation using laser Doppler flowmetry, and iii) reactivity of isolated cerebral arteries using online videomicroscopy. Aβ burden was quantified by ELISA and immunostaining, while other AD landmarks were measured by western blot and immunohistochemistry. Results B1R protein levels were increased in APP mouse hippocampus and, prominently, in reactive astrocytes surrounding Aβ plaques. In APP mice, B1R antagonism with SSR240612 improved spatial learning, memory and normalized protein levels of the memory-related early gene Egr-1 in the dentate gyrus of the hippocampus. B1R antagonism restored sensory-evoked CBF responses, endothelium-dependent dilations, and normalized cerebrovascular protein levels of endothelial nitric oxide synthase and B2R. In addition, SSR240612 reduced (approximately 50%) microglial, but not astroglial, activation, brain levels of soluble Aβ1-42, diffuse and dense-core Aβ plaques, and it increased protein levels of the Aβ brain efflux transporter lipoprotein receptor-related protein-1 in cerebral microvessels. Conclusion These findings show a selective upregulation of astroglial B1R in the APP mouse brain, and the capacity of the B1R antagonist to abrogate amyloidosis, cerebrovascular and

  15. Stability of aflatoxin B1 in animal feed candidate reference materials

    NARCIS (Netherlands)

    Roos, A.H.; Mazijk, van R.J.; Tuinstra, L.G.M.T.; Huf, F.A.

    1991-01-01

    Two candidate reference materials animal feed were stored at a temperature of -18°C, 4 C, 20°C and 37°C. The stability of aflatoxin B1 was studied duringa period of two years. A significant decrease in the aflatoxin B1 content was measured in the samples stared at 20°C and 37°C. In the samples

  16. Molecular characterization of cytochrome P450 1B1 and effect of ...

    African Journals Online (AJOL)

    Furthermore, real-time PCR was used for measuring BaP induction of CYP1B1 mRNA in different organs of tilapia (O. niloticus), using β-actin gene as internal control, and the results revealed that there was a large increase in CYP1B1 mRNA in liver (22.8), intestine (2.0) and muscles (1.3). Keywords: Oreochromis niloticus ...

  17. Determining the frequencies of B1, B2, B3 and E alleles of the ...

    African Journals Online (AJOL)

    The allelic frequencies of the B1, B2, B3 and E alleles were 0.927, 0.073, 0.390, and 0.272, respectively. B1 and B2 alleles did not affect milk yield and composition. B3 allele had significant effects on protein, fat, total solid (TS), solid not fat (SNF), casein and lactose percentages, but not on lactose yield. E allele significantly ...

  18. Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant.

    Directory of Open Access Journals (Sweden)

    Vahid Omidvar

    Full Text Available The 7B-1 tomato (Solanum lycopersicum L. cv Rutgers is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 7B-1, transcriptomic profiles of the 7B-1 male-sterile and wild type (WT anthers were studied using mRNA sequencing (RNA-Seq. In total, 768 differentially expressed genes (DEGs were identified, including 132 up-regulated and 636 down-regulated transcripts. Gene ontology (GO enrichment analysis of DEGs suggested a general impact of the 7B-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 7B-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD of tapetal cells in 7B-1 anthers. The present study provides insights into the transcriptome of the 7B-1 mutant. We identified several genes with altered expression level in 7B-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.

  19. Cloning and tissue expression of cytochrome P450 1B1 and 1C1 ...

    African Journals Online (AJOL)

    Cytochrome P450 1 (CYP1) is widely used as an indicator of exposure to environmental contaminants. In the study, two full-length complementary DNAs encode for CYP1B1 and CYP1C1 were cloned from medaka liver exposed to 500 ppb β-naphthoflavone for 24 h. CYP1B1, having 1984 bp, contains an open reading ...

  20. Code-B-1 for stress/strain calculation for TRISO fuel particle (Contract research)

    International Nuclear Information System (INIS)

    Aihara, Jun; Ueta, Shohei; Shibata, Taiju; Sawa, Kazuhiro

    2011-12-01

    We have developed Code-B-1 for the prediction of the failure probabilities of the coated fuel particles for the high temperature gas-cooled reactors (HTGRs) under operation by modification of an existing code. A finite element method (FEM) is employed for the stress calculation part and Code-B-1 can treat the plastic deformation of the coating layer of the coated fuel particles which the existing code cannot treat. (author)

  1. Role of CYP1B1 in PAH-DNA adduct formation and breast cancer risk

    Energy Technology Data Exchange (ETDEWEB)

    Goth-Goldstein, Regine; Russell, Marion L.; Muller, A.P.; Caleffi, M.; Eschiletti, J.; Graudenz, M.; Sohn, Michael D.

    2010-04-01

    This study investigated the hypothesis that increased exposure to polycyclic aromatic hydrocarbons (PAHs) increases breast cancer risk. PAHs are products of incomplete burning of organic matter and are present in cigarette smoke, ambient air, drinking water, and diet. PAHs require metabolic transformation to bind to DNA, causing DNA adducts, which can lead to mutations and are thought to be an important pre-cancer marker. In breast tissue, PAHs appear to be metabolized to their cancer-causing form primarily by the cytochrome P450 enzyme CYP1B1. Because the genotoxic impact of PAH depends on their metabolism, we hypothesized that high CYP1B1 enzyme levels result in increased formation of PAH-DNA adducts in breast tissue, leading to increased development of breast cancer. We have investigated molecular mechanisms of the relationship between PAH exposure, CYP1B1 expression and breast cancer risk in a clinic-based case-control study. We collected histologically normal breast tissue from 56 women (43 cases and 13 controls) undergoing breast surgery and analyzed these specimens for CYP1B1 genotype, PAH-DNA adducts and CYP1B1 gene expression. We did not detect any difference in aromatic DNA adduct levels of cases and controls, only between smokers and non-smokers. CYP1B1 transcript levels were slightly lower in controls than cases, but the difference was not statistically significant. We found no correlation between the levels of CYP1B1 expression and DNA adducts. If CYP1B1 has any role in breast cancer etiology it might be through its metabolism of estrogen rather than its metabolism of PAHs. However, due to the lack of statistical power these results should be interpreted with caution.

  2. Picking the Bone: The B-1 Bomber as a Platform for Innovation

    Science.gov (United States)

    2010-06-01

    five sections, an introduction, a conclusion, and three main body chapters that provide an historical narrative of the weapon system and highlights...the weapon‘s computer. Once the handoff occurred and the weapon exited the bomb bay, special lift-producing strakes on the munition‘s body increased...SD III- 38. Memorandum For ASC/HO. From Roserika Kenndy, Deputy B-1 Sytem Program Director. 4 December 2001. B-1 Conventional Mission Upgrade

  3. Residues of Avermectin B1a in rotational crops and soils following soil treatment with [14C]Avermectin B1a

    International Nuclear Information System (INIS)

    Moye, H.A.; Malagodi, M.H.; Yoh, H.; Leibee, G.L.; Ku, C.C.; Wislocki, P.G.

    1987-01-01

    [ 14 C]Avermectin B 1 a was applied twelve times to muck and sandy loam soils and three times to sandy soil at 0.025-0.030 lb/acre per application. These applications simulated the intended use of avermectin B 1 a on celery, vegetables, and cotton, respectively. Following three aging periods in each soil type, sorghum, lettuce, and carrot or turnip seeds were planted and harvested at one-fourth, half, and full size. Analysis of these crops by oxidative combustion demonstrated that crops grown in muck, sandy loam, and sandy soils contained radiolabeled residues ranging from below the limit of quantitation (BLQ) to 7.4 μg/kg of avermectin B 1 a equivalents, BLQ to 11.6 μg/kg, and BLQ to 3.54 μg/kg, respectively. There was a general trend of decreasing residue concentrations with increasing preharvest intervals in crops grown in all soils. The radioactivity present in muck and sandy loam soils disappeared with half-lives ranging from 103 to 267 days and from 102 to 132 days, respectively

  4. Evolutionary implication of B-1 lineage cells from innate to adaptive immunity.

    Science.gov (United States)

    Zhu, Lv-yun; Shao, Tong; Nie, Li; Zhu, Ling-yun; Xiang, Li-xin; Shao, Jian-zhong

    2016-01-01

    The paradigm that B cells mainly play a central role in adaptive immunity may have to be reevaluated because B-1 lineage cells have been found to exhibit innate-like functions, such as phagocytic and bactericidal activities. Therefore, the evolutionary connection of B-1 lineage cells between innate and adaptive immunities have received much attention. In this review, we summarized various innate-like characteristics of B-1 lineage cells, such as natural antibody production, antigen-presenting function in primary adaptive immunity, and T cell-independent immune responses. These characteristics seem highly conserved between fish B cells and mammalian B-1 cells during vertebrate evolution. We proposed an evolutionary outline of B cells by comparing biological features, including morphology, phenotype, ontogeny, and functional activity between B-1 lineage cells and macrophages or B-2 cells. The B-1 lineage may be a transitional cell type between phagocytic cells (e.g., macrophages) and B-2 cells that functionally connects innate and adaptive immunities. Our discussion would contribute to the understanding on the origination of B cells specialized in adaptive immunity from innate immunity. The results might provide further insight into the evolution of the immune system as a whole. Copyright © 2015 Elsevier Ltd. All rights reserved.

  5. Plasma extravasation mediated by lipopolysaccharide-induction of kinin B1 receptors in rat tissues

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Wille

    2001-01-01

    Full Text Available The present study was performed to: (a evaluate the effects of kinin B1 (Sar{D-Phe8}-des-Arg9-BK; 10 nmol/kg and B2 (bradykinin (BK; 10 nmol/kg receptor agonists on plasma extravasation in selected rat tissues; (b determine the contribution of a lipopolysaccharide (LPS (100 μ g/kg to the effects triggered by B1 and B2 agonists; and (c characterize the selectivity of B1 ({Leu8}desArg9-BK; 10 nmol/kg and B2 (HOE 140; 10 nmol/kg antagonists as inhibitors of this kinin-induced phenomenon. B1 and B2 agonists were shown to increase plasma extravasation in the duodenum, ileum and also in the urinary bladder of the rat. LPS pretreatment enhanced the plasma extravasation mediated only by the B1 agonist in the duodenum, ileum, trachea, main and segmentar bronchi. These effects were prevented by the B1. but not the B2 antagonist. In normal rats, the B2 antagonist inhibited the effect of B2 agonist in all the tissues analyzed. However, in LPS-treated rats, the B2 antagonist was ineffective in the urinary bladder.

  6. Silencing of Atp2b1 increases blood pressure through vasoconstriction.

    Science.gov (United States)

    Shin, Young-Bin; Lim, Ji Eun; Ji, Su-Min; Lee, Hyeon-Ju; Park, So-Yon; Hong, Kyung-Won; Lim, Mihwa; McCarthy, Mark I; Lee, Young-Ho; Oh, Bermseok

    2013-08-01

    Recent genome-wide association studies (GWASs) have identified 30 genetic loci that regulate blood pressure, increasing our understanding of the cause of hypertension. However, it has been difficult to define the causative genes at these loci due to a lack of functional analyses. In this study, we aimed to validate the candidate gene ATP2B1 in 12q21, variants near which have the strongest association with blood pressure in Asians and Europeans. ATP2B1 functions as a calcium pump to fine-tune calcium concentrations - necessary for repolarization following muscular contractions. We silenced Atp2b1 using an siRNA complex, injected into mouse tail veins. In treated mice, blood pressure rose and the mesenteric arteries increased in wall : lumen ratio. Moreover, the arteries showed enhanced myogenic responses to pressure, and contractile responses to phenylephrine increased compared with the control, suggesting that blood pressure is regulated by ATP2B1 through the contraction and dilation of the vessel, likely by controlling calcium concentrations in the resting state. These results support that ATP2B1 is the causative gene in the blood pressure-associated 12q21 locus and demonstrate that ATP2B1 expression in the vessel influences blood pressure.

  7. A Novel Role for C5a in B-1 Cell Homeostasis

    Directory of Open Access Journals (Sweden)

    Katharina Bröker

    2018-02-01

    Full Text Available B-1 cells constitute a unique subpopulation of lymphocytes residing mainly in body cavities like the peritoneal cavity (PerC but are also found in spleen and bone marrow (BM. As innate-like B cells, they mediate first line immune defense through low-affinity natural IgM (nIgM antibodies. PerC B-1 cells can egress to the spleen and differentiate into nIgM antibody-secreting plasma cells that recognize conserved exogenous and endogenous cellular structures. Homing to and homeostasis within the PerC are regulated by the chemokine CXCL13 released by PerC macrophages and stroma cells. However, the exact mechanisms underlying the regulation of CXCL13 and B-1 homeostasis are not fully explored. B-1 cells play important roles in the inflammatory response to infection, autoimmunity, ischemia/reperfusion injury, obesity, and atherosclerosis. Remarkably, this list of inflammatory entities has a strong overlap with diseases that are regulated by complement suggesting a link between B-1 cells and the complement system. Interestingly, up to now, no data exist regarding the role of complement in B-1 cell biology. Here, we demonstrate for the first time that C5a regulates B-1 cell steady-state dynamics within the peritoneum, the spleen, and the BM. We found decreased B-1a cell numbers in the peritoneum and the spleen of C5aR1−/− mice associated with increased B1-a and B1-b numbers in the spleen and high serum titers of nIgM antibodies directed against phosphorylcholine and several pneumococcal polysaccharides. Similarly, peritoneal B-1a cells were decreased in the peritoneum and splenic B-1a and B-1b cells were increased in C5aR2−/− mice. The decrease in peritoneal B-1 cell numbers was associated with decreased peritoneal CXCL13 levels in C5aR1−/− and C5aR2−/− mice. In search for mechanisms, we found that combined TLR2 and IL-10 receptor activation in PerC macrophages induced strong CXCL13 production, which was significantly reduced in cells

  8. Travestie im alten Ägypten

    Directory of Open Access Journals (Sweden)

    prof.Magda Abdalla

    2011-01-01

    Full Text Available Der Artikel möchte versuchen zu beweisen, dass den alten Ägyptern Travestie bekannt war. In verschiedenen Texten wie Märchen, Fabeln und Liebesgedichten auf Papyri und Ostraka, finden sich Belege von Travestie. Zuerst muss jedoch betont werden, dass es einen Unterschied zwischen dem Begriff der Travestie, mit anderen Worten der Verkleidung,[1] und dem der Metamorphose, dem Verwandeln der Gestalt,[2] gibt. [1] Tra.ve´stie: f. satirisch, Verspottung eines Literaturwerkes, bei der (im Unterschied zur Parodie der Inhalt beibehalten und die Form verändert wird (frz. travestie „Verkleidung“; zu lat. trans „hinüber“+vestire, „kleiden“. G. Wahrig,. Deutsches Wörterbuch, mit einem „Lexikon der deutschen Sprachlehre“( München, 1987, 1292. Es gibt auch eine moderne Definition des Begriffes Travestie aus dem 17. Jahrhundert. Hier beinhaltet diese ein Verfahren oder eine kritische Zielsetzung.w.Karrer.,Parodie Travestie, Pastiche (München, 1977, 19, 46. [2] Me.ta.mor´pho.se Umwandlung eines Gesteins in ein anderes; Wandlung des jungen Tieres durch verschiedene äußere Stadien; Verwandlung von Menschen in Tiere, Pflanzen, Quellen usw. (Deutsches Wörterbuch, 883. الإحصائيات

  9. Das Problem der Ethnogenese im antiken Griechenland

    Directory of Open Access Journals (Sweden)

    Welwei Karl-Wilhelm

    2011-01-01

    Full Text Available Sprachwissenschaftliche Untersuchungen lassen darauf schließen, dass die historischen Dialekte der alten Hellenen erst auf griechischem Boden um und nach 1000 v. Chr. entstanden sind. Bei den frühen Zuwanderern kann es sich nur um kleinere Verbände gehandelt haben, so daß zweifelllos keine großen „Stämme“ nach Griechenland vorgedrungen sind, wie man in der älteren Forschung angenommen hat. In Griechenland haben die Zuwanderer die Suffixe -ss und -nth oder -nd nicht erst kennengelernt, sondern zumindest zum Teil schon mitgebracht und vermutlich im 3. Jahrtausend v. Chr. die allmähliche Entwicklung des späteren Griechischen eingeleitet. Impulse für die Entwicklung der materiellen Kultur gingen um 1700 v. Chr. von Kreta aus und beeinflussten in starkem Maße die mykenische Zeit mit ihren Palastsystemen, deren Zusammenbruch um 1200 v. Chr. aber nicht schon zum Ende der mykenischen Kultur führte, wenn auch die Linear B-Schrift nicht mehr benutzt wurde und die Infrastrukturen der Machtzentren zusammenbrachen. Dies war zugleich die Voraussetzung für die Bildung kleinerer Siedlungen mit neuen Führungssystemen und Sozialstrukturen sowie mit einem jeweils eigenen Identitätsbewußtsein.

  10. Measurement of plasma flows in IMS

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, B.J. [Kyoto Univ., Uji (Japan); Talmadge, J.N.; Anderson, D.T.; Anderson, F.S.B.; Matthews, P.G.; Shohet, J.L. [Univ. of Wisconsin, Madison, WI (United States)

    1995-04-01

    Mach probe measurements of bias-induced ion flows were made in the Interchangeable Module Stellarator (IMS) as a function of neutral pressure and viscosity (which increases with minor radius) and compared to a fluid theory model. Using a probe model for an unmagnetized plasma, the poloidal flow speed measured with a Mach probe agrees with that calculated from momentum balance to within 15%. The dependencies of the measured ion flow magnitudes and decay rates on neutral pressure and viscosity as predicted by the theory are qualitatively observed in the experimental measurements, clearly demonstrating the effects of both ion-neutral collisions and viscosity in the damping of the bias-induced flows. However, the measured flow direction is nearly poloidal, while the theory predicts a predominantly Pfirsch-Schlueter-like toroidal flow. Also the two-dimensional variation at a constant toroidal angle of the parallel electron current was measured in an unbiased plasma. The measured profiles demonstrate the dependence of the current on both the radial pressure gradient and the cosine of the poloidal angle, as predicted by theory. 11 refs., 5 figs.

  11. Brain tumors in childhood; Hirntumoren im Kindesalter

    Energy Technology Data Exchange (ETDEWEB)

    Sinzig, M.; Gasser, J.; Hausegger, K.A. [Landeskrankenhaus Klagenfurt, Kinderradiologie RZI, Klagenfurt (Austria); Jauk, B. [Landeskrankenhaus Klagenfurt, Abt. fuer Kinder- und Jugendheilkunde, Klagenfurt (Austria)

    2008-10-15

    Central nervous system (CNS) tumors are the most common solid neoplasms in childhood and the second most common malignancies after leukemia in the pediatric age group. Supratentorial tumors are more common in children younger than 2 years old and in adolescents, whereas in patients between 2 and 12 years of age brain tumors originating in the posterior fossa dominate. This implies a relationship between the type of tumor, its location and the age of the patient, which has to be considered in differential diagnoses. Medulloblastoma represents the most common malignant brain tumor in childhood. In the posterior fossa medulloblastomas are approximately as frequent as astrocytomas. Supratentorial astrocytomas are by far the main tumor type. In this report some typical CNS neoplasms in children are discussed and their neuroradiological features are demonstrated. (orig.) [German] Hirntumoren sind die haeufigsten soliden Tumoren des Kindesalters und repraesentieren nach den Leukaemien die zweithaeufigsten malignen Erkrankungen bei Kindern. Waehrend bei Kleinkindern und Adoleszenten supratentorielle Hirntumoren ueberwiegen, ist bei Patienten zwischen 2 und 12 Jahren haeufiger die hintere Schaedelgrube Ursprungsort dieser Malignome. Daraus geht hervor, dass gewisse Tumortypen eine gewisse Alterspraedilektion aufweisen, was neben der radiologischen Morphologie der Raumforderung fuer differenzialdiagnostische Ueberlegungen ueberaus hilfreich sein kann. Das Medulloblastom ist das haeufigste ZNS-Malignom des Kindesalters und repraesentiert zusammen mit zerebellaeren Astrozytomen auch den haeufigsten Tumortyp der hinteren Schaedelgrube. Supratentoriell stehen die Astrozytome ganz im Vordergrund. In dieser Arbeit werden einige typische kindliche infra- und supratentorielle Hirntumoren diskutiert und ihre neuroradiologischen Merkmale dargestellt. (orig.)

  12. Allergens in indoor spaces; Allergene im Innenraumbereich

    Energy Technology Data Exchange (ETDEWEB)

    Wahn, U. [Universitaetskinderklinik, FU Berlin (Germany)

    1994-02-01

    Research into environmental factors and how they affect the health of children needs to address a number of questions: Does the environment play any part in this at all? What are the affecting environmental factors? What is the scope of specific environmental factors relative to an induction or an enhancement of a disease? The search for and identification of allergens has priority relative to allergic developments in children. The author describes major allergen sources, the pathogenesis of allergies, the analysis of allergens and a study conducted by several pediatric clinics on the development of allergies in infants and young children, while focussing on risk factor of house-dust mites and options to reduce the exposure to mite-associated allergens. (Uhe) [Deutsch] Bei der Erforschung von Umweltfaktoren und ihrem Einfluss auf die Gesundheit von Kindern interessieren mehrere Fragen: spielt die Umwelt ueberhaupt ein Rolle? Welche Umwelteinfluesse sind es, wie gross ist die krankheitsindzierende oder krankheitsverstaerkte Rolle bestimmter Umwelteinfluesse? Fuef die Allergieentwicklung bei Kindern stehen die Allergene an erster Stelle. Neben der Beschreibung der wichtigsten Allergiequellen und der Entstehung von Allergien und Analytik von Allergenen wird ausfuehrlich eine Studie verschiedener Kinderkliniken zur Allergieentwicklung im Kindesalter beschrieben. Dabei wird vor allem auf den Risikofaktor Hausstaubmilbe und die Moeglichkeiten der Reduzierung der Milbenallergenbelastung eingegangen. (Uhe)

  13. Implementing Adaptive Educational Methods with IMS Learning Design

    NARCIS (Netherlands)

    Specht, Marcus; Burgos, Daniel

    2006-01-01

    Please, cite this publication as: Specht, M. & Burgos, D. (2006). Implementing Adaptive Educational Methods with IMS Learning Design. Proceedings of Adaptive Hypermedia. June, Dublin, Ireland. Retrieved June 30th, 2006, from http://dspace.learningnetworks.org

  14. [Preussen und Livland im Zeichen der Reformation] / Anti Selart

    Index Scriptorium Estoniae

    Selart, Anti, 1973-

    2015-01-01

    Arvustus: Preussen und Livland im Zeichen der Reformation. Hrsg. von Arno Mentzel-Reuters und Klaus Neitmann. (Tagungsberichte der Historischen Kommission für ost- und westpreussische Landesforschung, 28). Fibre Verlag. Osnabrück 2014

  15. Lexikon lesbischer Frauen im Film von Daniela Sobek

    Directory of Open Access Journals (Sweden)

    Karin Windt

    2002-03-01

    Full Text Available Das Lexikon stellt ein neues Basiswerk über lesbische Figuren im Film dar. Neben zahlreichen Artikeln von Anbeginn der Filmgeschichte bis heute gibt es Features über bekannte Schauspielerinnen und lesbische role models.

  16. A Management Tool for Improvement (Improving) IMS Stations Performance

    International Nuclear Information System (INIS)

    Mohammad, D.; Pantin, A.; Quintana, E.

    2011-01-01

    IMS-Maintenance is a software under development by the personnel of ARN-CTBT office, intended for the technical management of IMS stations under ARN's responsibility. This software integrates the whole control of the equipment in an IMS station, including the management of consumables used in daily or periodic operation. It permits an easy retrieval (access) of the technical features, suppliers, etc. of each item of the equipment, as well as its maintenance history and scheduled task. Although this project is still in a starting phase, a preliminary version of this software is being used in radionuclide station RN01 (Buenos Aires), having already demonstrated its usefulness and potential for the improvement of IMS station management. (authors)

  17. Mapping IMS Learning Design and Moodle. A first understanding

    NARCIS (Netherlands)

    Burgos, Daniel

    2006-01-01

    Burgos, D. (2006). Mapping IMS Learning Design and Moodle. A first understanding. Presentation at the 8th International Simposio of Educational Computer Science. October, 24-27, 2006, León, Spain: IEEE Technical Committee on Learning Technology.

  18. Anwendung der Prozeßkostenrechnung im Dienstleistungsbereich - Fallbeispiel Logistik

    OpenAIRE

    Coenenberg, Adolf G.

    2002-01-01

    Anwendung der Prozeßkostenrechnung im Dienstleistungsbereich - Fallbeispiel Logistik / A. G. Coenenberg ; U. Specht ; A. Kock. - In: Wertschöpfungsmanagement als Kernkompetenz / hrsg. von Horst Albach ... - Wiesbaden : Gabler, 2002. - S. 493-525

  19. IMS Learning Design: defining collaborative & multi-role learning experiences

    NARCIS (Netherlands)

    Tattersall, Colin

    2004-01-01

    This presentation was during the eLearning Results summit in Sestri Levante in May 2004. After introducing the central concepts of IMS LD, a worked example is taken to illustrate various aspects of the specifiction.

  20. Medienerziehung im Vorschulbereich - Zum Projekt Mediengarten der Wiener Medienpädagogik

    Directory of Open Access Journals (Sweden)

    Gudrun Kern

    2010-09-01

    Full Text Available Im Zuge des Projekts Mediengarten der Wiener Medienpädagogik wurde in Kooperation mit angehenden KindergartenpädagogInnen eine Medienerziehung im Sinne einer Auseinandersetzung über Medien im Vorschulbereich anhand konkreter Angebote in Kindergärten konzipiert. Im folgenden Artikel werden die Schwerpunktsetzungen dieses Konzepts vorgestellt und durch praktische Beispiele verdeutlicht.

  1. [B1a and b1b evolutionary branch of coxsackie virus A16 co-prevailed in Inner Mongolia Autonomous Region].

    Science.gov (United States)

    Tian, Xiao-Ling; Zhang, Yong; Song, Zhuang-Zhi; Fan, Yao-Chun; Ma, Xue-En; Wang, Wen-Rui

    2013-06-01

    To study on the molecular evolution of Coxsackie virus A16 (CVA16)isolated from clinical speci-mens of Hand, foot and mouth Disease( HFMD) patients in Inner Mongolia in 2010. A total of 921 clinical specimens including stools, throat swabs and vesicle fluids were collected from 888 HFMD patients in out-patient service in Inner Mongolia and viral isolation was then performed, the positive viral isolates were identified by using the real-time PCR method detecting CVA16. A total of 50 CVA16 isolates were selected from the patients presenting mild symptoms, severe symptoms and the death patients randomly, and the VP1 coding regions of representative CVA16 isolates were amplified and sequenced. Finally the phylogenetic tree was constructed among the VP1 coding regions of the different genotypes and subgenotypes of CVA16 strains. Eighty two viruses were isolated form 921 clinical specimens, the positive rate was 8. 90%, of which 3 viruses were isolated from severe cases and 1 viruses was from death cases. The nucleotide acid of 50 representative CVA16 strains in Inner Mongolia were closed to CVA16 strains isolated from mainland China since 1998, especially from Beijing in 2009 and from Henan in 2010, the identity were 96. 18% approximately 98. 88% and 94. 94a approximately 98. 76%, respectively. There was a little difference in the nucleotide acid between the CVA16 strains from Inner Mongolia in 2010 and in 2007, the identity were 91. 68% approximately 96. 52% The phylogenetic tree showed that all CVA16 strains clustered within Bla and B1b evolution branch of B1 genotype. There was slight difference in the nucleotide and the amino acid in VP1 region among the 50 Inner Mongolia CVA16 strains, the identity were 89. 99% approximately 100% and 98. 31% approximately 100%, respectively, indicating that these strains belonged to many different viral transmission chains. The CVA16 strains circulated in Inner Mongolia in 2010 were all belong to B1a and B1b evolution branch of B1

  2. Untersuchung zum Einsatz deutscher Lehramts-Praktikanten im universitaeren Unterricht

    Directory of Open Access Journals (Sweden)

    Seval KARACABEY

    2017-12-01

    Full Text Available In dem Beitrag werden Untersuchungsergebnisse vorgestellt, die sich mit dem Einsatz von Lehramts-Praktikanten aus Deutschland an der Universität Mugla Sıtkı Koçman im Rahmen der Deutschlehrerausbildung befassen. Mit der Arbeit soll nachgewiesen werden, dass sich die Motivation der türkischen Studierenden zum Deutschlernen durch die direkte Begegnung mit deutschen Lehramts-Praktikanten positiv beeinflusst werden kann. In diesem Zusammenhang wurde der Beitrag deutscher Lehramts-Praktikanten auf die Motivation der türkischen DaF-Studierenden ausgehend von den Studentenmeinungen untersucht. Generell fällt es türkischen Studierenden schwer, sich in der Fremdsprache zu äußern, zu sprechen oder zu schreiben, sobald man über Standardsituationen des Alltagskommunikation hinausgeht. Ein Grund dafür ist das Fehlen von Sprachkompetenz, aber auch von Erfahrungen bei konkreten Sprechanlässen und Sprechsituationen. Der Einsatz von Lehramts-Praktikanten, die im Rahmen des Erasmus-Programs im universitären Unterricht assistieren, soll diesem Mangel entgegen wirken. Die Studierenden sollen dabei unterstüzt werden, sich in der Fremdsprache zu äußern. Mit vorliegender Untersuchung wird der Frage nachgegangen, ob der Einsatz von Lehramts-Praktikanten aus Deutschland im universitären DaF-Unterricht in der Türkei das Interesse zum Deutschlernen fördert. Um den Beitrag deutscher Lehramts-Praktikanten im Rahmen des Erasmus-Programms im universitären Unterricht auf die Motivation türkischer Lehramtskandidaten zu ermitteln, wurde am Ende des Praktikums eine schriftliche Befragung durchgeführt. Die Form der Befragung war halbstrukturiert. Die Daten wurden mit einer qualitativen Methode ausgewertet. Ausgehend von den Ergebnissen kann konstatiert werden, dass die Lehramts-Praktikanten für eine reale Kommunikationssituation im fremdsprachlichen Unterricht sorgen, dass die türkischen DaF-Studierenden sprachliche Fortschritte und Fortschritte im Rahmen

  3. Schutz vor Vogelfraß im ökologischen Ackerbau - Vogelscheuche im Tiefflug

    OpenAIRE

    Mücke, Markus

    2001-01-01

    Im ökologischen Ackerbau bereiten Schäden durch Vogelfraß besonders an den Kulturen Erbsen, Mais und Getreide erhebliche Probleme. Verursacher sind hier hauptsächlich Krähen und Tauben. Seit rund drei Jahren wird eine Vogelscheuche in Form eines Drachen auf dem Markt angebo-ten. Das Grundprinzip dieser Vogelscheuche nutzt den angeborenen Instinkt der Vögel, nicht in der Nähe eines schwebenden Raubvogels zu fressen. Deshalb ähnelt der Drachen in seiner äußeren Form schemenhaft einem Greif...

  4. Background Acoustic Noise Models for the IMS Hydroacoustic Stations

    Science.gov (United States)

    2010-09-01

    BACKGROUND ACOUSTIC NOISE MODELS FOR THE IMS HYDROACOUSTIC STATIONS Philip E. Harben and Terri F. Hauk Lawrence Livermore National Laboratory ...levels in the ocean have been increasing for the past several decades (McDonald, 2006) yet many of our hydroacoustic detection assessment tools use...dB since the 1960’s. To address this issue and provide accurate noise models at each of the six International Monitoring System (IMS) hydroacoustic

  5. CORPORATIVE MOTIVES ON IMPLEMENTATION OF INTEGRATED MANAGEMENT SYSTEM (IMS

    Directory of Open Access Journals (Sweden)

    Dragan Rajkovic

    2009-09-01

    Full Text Available Integration of management systems for quality, environment, health and risk management as well as corporative social responsibilities is workable corporative approach to reduce costs, effective use of resources, higher motivation of employees and better fulfillment of requirements of social engagements and stakeholders. This paper presents contents of literature and review of a company motives on integrated management system (IMS implementation, namely factors affecting the IMS implementation.

  6. Translating the IM behaviour of munitions to operational consequences

    OpenAIRE

    Hooijmeijer, P.A.; Scholtes, J.H.G.; Voort, M.M. van der

    2012-01-01

    A recent study was dedicated to relate the behaviour of Insensitive Munitions (IM) to an operational context. At TNO, a toolbox with a series of engineering tools is under study. These tools focus on the mechanisms that occur due to a range of IM threats and that transfer the effects from a reacting (donor) munition article to the neighbouring (acceptor) munition articles, focusing on sympathetic reaction. The consequences of this mechanism could be devastating, resulting in a mass detonation...

  7. Metropolitan (Im)migrants in the "Lettered City"

    OpenAIRE

    Balkan, Stacey

    2012-01-01

    In her article "Metropolitan (Im)migrants in the 'Lettered City'" Stacey Balkan employs Ángel Rama's discussion of audience as a means of analyzing a Latin American diaspora that exists beyond the "rational periphery" of the state. Herein, the term diaspora is redefined as a translocal phenomenon wherein the metropolitan (im)migrant moves from rural margin to urban center. Normative definitions of exile — persons displaced from autonomous nation-states — are likewise scrutinized in the contex...

  8. Expansion of B-1a cells with germline heavy chain sequence in lupus mice

    Directory of Open Access Journals (Sweden)

    Nichol E Holodick

    2016-03-01

    Full Text Available B6.Sle1.Sle2.Sle3 (B6.TC lupus-prone mice carrying the NZB allele of Cdkn2c, encoding for the cyclin-dependent kinase inhibitor P18INK4, accumulate B-1a cells due to a higher rate of proliferative self-renewal. However, it is unclear whether this affects primarily early appearing B-1a cells of fetal origin or later appearing B-1a cells that emerge from bone marrow. B-1a cells are the major source of natural autoantibodies, and it has been shown that their protective nature is associated with a germline-like sequence, which is characterized by few N-nucleotide insertions and a repertoire skewed towards rearrangements predominated during fetal life, VH11 and VH12. To determine the nature of B-1a cells expanded in B6.TC mice, we amplified immunoglobulin genes by PCR from single cells in mice. Sequencing showed a significantly higher proportion of B-1a cell antibodies display fewer N-additions in B6.TC mice than in B6 control mice. Following this lower number of N-insertions within the CDR-H3 region, the B6.TC B-1a cells display shorter CDR-H3 length than B6 B-1a cells. The absence of N-additions is a surrogate for fetal origin, as TdT expression starts after birth in mice. Therefore, our results suggest that the B-1a cell population is not only expanded in autoimmune B6.TC mice but also qualitatively different with the majority of cells from fetal origin. Accordingly, our sequencing results also demonstrated overuse of VH11 and VH12 in autoimmune B6.TC mice as compared to B6 controls. These results suggest that the development of lupus autoantibodies in these mice is coupled with skewing of the B-1a cell repertoire and possible retention of protective natural antibodies.

  9. Overview of IMS infrasound station and engineering projects

    Science.gov (United States)

    Marty, J.; Doury, B.; Kramer, A.; Martysevich, P.

    2015-12-01

    The Provisional Technical Secretariat (PTS) of the Comprehensive Nuclear-Test-Ban Treaty (CTBTO) has a continuous interest in enhancing its capability in acoustic source detection, localization and characterization. The infrasound component of the International Monitoring System (IMS) constitutes the only worldwide ground-based infrasound network. It consists of sixty stations, among which forty-eight are already certified and continuously transmit data to the International Data Centre (IDC) in Vienna, Austria. Each infrasound station is composed of an array of infrasound sensors capable of measuring micro-pressure changes produced at ground level by infrasonic waves. The characteristics of infrasonic waves are computed in near real-time by IDC automatic detection software and are used as an input to IDC source categorization and localization algorithms. The PTS is continuously working towards the completion and sustainment of the IMS infrasound network. The objective of this presentation is to review the main activities performed in the IMS infrasound network over the last five years. This includes construction, installation, certification, major upgrade and revalidation activities. Major technology development projects to improve the reliability and robustness of IMS infrasound stations as well as their compliance with IMS Operational Manual requirements will also be presented. This includes advances in array geometry, wind noise reduction, system calibration, meteorological data as well as power and communication infrastructures. Finally the impact of all these changes on the overall detection capability of the IMS infrasound network will be highlighted.

  10. Thin layer chromatography-ion mobility spectrometry (TLC-IMS).

    Science.gov (United States)

    Ilbeigi, Vahideh; Tabrizchi, Mahmoud

    2015-01-06

    Ion mobility spectrometry (IMS) is a fast and sensitive analytical method which operates at the atmospheric pressure. To enhance the capability of IMS for the analysis of mixtures, it is often used with preseparation techniques, such as GC or HPLC. Here, we report for the first time the coupling of the thin-layer chromatography and IMS. A variety of coupling schemes were tried that included direct electrospray from the TLC strip tip, indirect electrospray from a needle connected to the TLC strip, introducing the moving solvent into the injection port, and, the simplest way, offline introduction of scratched or cut pieces of strips into the IMS injection port. In this study a special solvent tank was designed and the TLC strip was mounted horizontally where the solvent would flow down. A very small funnel right below the TLC tip collected the solvent and transferred it to a needle via a capillary tubing. Using the TLC-ESI-IMS technique, acceptable separations were achieved for two component mixtures of morphine-papaverine and acridine-papaverine. A special injection port was designed to host the pieces cut off the TLC. The method was successfully used to identify each spot on the TLC by IMS in a few seconds.

  11. Information management in intermodal transport; Informationsmanagement im Kombinierten Verkehr

    Energy Technology Data Exchange (ETDEWEB)

    Boese, J.W.; Voss, S. [Technische Univ. Braunschweig (Germany). Abt. Allgemeine Betriebswirtschaftslehre, Wirtschaftsinformatik und Informationsmanagement

    2000-07-01

    Transport systems of the future will be more efficient and less polluting than the current systems. Intermodal transport is an interesting alternative, i.e. truck/railway or truck/ship systems. In spite of extensive state funding, systems of this type still fail to meet the standards to make them competitive. The contribution compares monomodal and intermodal systems and attempts to defines strategies to make intermodal systems more successful. [German] Vor dem Hintergrund des in den vergangenen Jahren kontinuierlich gestiegenen Verkehrsaufkommens und den damit in engem Zusammenhang stehenden Umweltproblemen sind fuer die Zukunft leistungsfaehige Transportsysteme gefordert, die eine weiter wachsende Verkehrsnachfrage im Vergleich zu den zur Zeit im Einsatz befindlichen Systemen umweltvertraeglicher abwickeln. Eine aussichtsreiche Alternative stellt in diesem Zusammenhang der Kombinierte Verkehr (KV) als intermodale Verkehrsform von Lkw und Eisenbahn bzw. Schiff dar. Trotz einer Vielzahl in der Vergangenheit erfolgter staatlicher Foerdermassnahmen fehlt es dem KV nach wie vor in vielen Bereichen an der noetigen Wettbewerbsfaehigkeit, was sich in einem entsprechend niedrigen Marktanteil am Gueterverkehrsmarkt niederschlaegt. Im vorliegenden Beitrag wird dieser bei vordergruendiger Betrachtung zunaechst als Widerspruch anmutende Sachverhalt im Rahmen einer vergleichenden Analyse von verschiedenen monomodalen Transportsystemen und dem KV untersucht. Die dabei ermittelten Ergebnisse dienen im weiteren als Ausgangspunkt zur Diskussion von Ansaetzen, die ein modernes betriebliches Informationsmanagement im Hinblick auf eine Verbesserung der Akzeptanz des KV bietet. (orig.)

  12. Incidência de fumonisina B1, aflatoxinas B1, B2, G1 e G2, ocratoxina A e zearalenona em produtos de milho Occurrence of fumonisin B1, aflatoxins B1, B2, G1, and G2, ochratoxin A and zearalenone in corn products

    Directory of Open Access Journals (Sweden)

    Luciane Mie Kawashima

    2006-09-01

    Full Text Available Levantamentos de ocorrência de micotoxinas em alimentos foram realizados nas últimas duas décadas nas regiões Sudeste e Sul do Brasil. Levantamentos em alimentos comercializados em outras regiões têm-se limitado a aflatoxinas em amendoim e castanhas do Brasil. O presente trabalho pesquisou a presença de fumonisina B1, aflatoxinas B1, B2, G1 e G2, ocratoxina A e zearalenona em 74 amostras de produtos a base de milho adquiridas no comércio da cidade de Recife, PE, durante o período de 1999 a 2001. Fumonisina B1 foi determinada por cromatografia líquida de alta eficiência com detecção por fluorescência e as demais toxinas foram determinadas por cromatografia em camada delgada. Fumonisina B1 foi encontrada em 94,6% das amostras em concentrações variando de 20 a 8600 µg/kg. Apenas 5 amostras continham aflatoxina B1 e o teor máximo encontrado foi 20 µg/kg. Duas amostras ultrapassaram o limite de 20 µg/kg para a somatória das aflatoxinas B1, B2, G1 e G2 (farinha de milho pré-cozida com 21,5 µg/kg e quirera (xerém com 23,3 µg/kg. As aflatoxinas G1 e G2, ocratoxina A e zearalenona não foram detectadas em nenhuma das amostras. Todas as amostras contaminadas com aflatoxinas também apresentaram fumonisina B1.Research concerning the presence of mycotoxin in food has been conducted in the Southwest and South regions of Brazil over the last two decades. Research in other regions has been limited to aflatoxin in peanuts and Brazil nuts. The aim of this work is to study the presence of fumonisin B1, aflatoxins B1, B2, G1, and G2, ochratoxin A and zearalenone in 74 samples of corn products acquired in shops and food markets in the city of Recife (PE from 1999 to 2001. Fumonisin B1 was determined by high performance liquid chromatography and fluorescence was detected. The other toxins were determined by thin layer chromatography. Fumonisin B1 was found in 94.6% of the samples in levels from 20 to 8600 µg/kg. Only 5 samples contained

  13. Mutations in SULT2B1 Cause Autosomal-Recessive Congenital Ichthyosis in Humans.

    Science.gov (United States)

    Heinz, Lisa; Kim, Gwang-Jin; Marrakchi, Slaheddine; Christiansen, Julie; Turki, Hamida; Rauschendorf, Marc-Alexander; Lathrop, Mark; Hausser, Ingrid; Zimmer, Andreas D; Fischer, Judith

    2017-06-01

    Ichthyoses are a clinically and genetically heterogeneous group of genodermatoses associated with abnormal scaling of the skin over the whole body. Mutations in nine genes are known to cause non-syndromic forms of autosomal-recessive congenital ichthyosis (ARCI). However, not all genetic causes for ARCI have been discovered to date. Using whole-exome sequencing (WES) and multigene panel screening, we identified 6 ARCI-affected individuals from three unrelated families with mutations in Sulfotransferase family 2B member 1 (SULT2B1), showing their causative association with ARCI. Cytosolic sulfotransferases form a large family of enzymes that are involved in the synthesis and metabolism of several steroids in humans. We identified four distinct mutations including missense, nonsense, and splice site mutations. We demonstrated the loss of SULT2B1 expression at RNA and protein levels in keratinocytes from individuals with ARCI by functional analyses. Furthermore, we succeeded in reconstructing the morphologic skin alterations in a 3D organotypic tissue culture model with SULT2B1-deficient keratinocytes and fibroblasts. By thin layer chromatography (TLC) of extracts from these organotypic cultures, we could show the absence of cholesterol sulfate, the metabolite of SULT2B1, and an increased level of cholesterol, indicating a disturbed cholesterol metabolism of the skin upon loss-of-function mutation in SULT2B1. In conclusion, our study reveals an essential role for SULT2B1 in the proper development of healthy human skin. Mutation in SULT2B1 leads to an ARCI phenotype via increased proliferation of human keratinocytes, thickening of epithelial layers, and altered epidermal cholesterol metabolism. Copyright © 2017 American Society of Human Genetics. Published by Elsevier Inc. All rights reserved.

  14. Scavenger receptor B1 facilitates macrophage uptake of silver nanoparticles and cellular activation

    Energy Technology Data Exchange (ETDEWEB)

    Aldossari, Abdullah A.; Shannahan, Jonathan H. [The University of Colorado Anschutz Medical Campus, Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences (United States); Podila, Ramakrishna [Clemson University, Department of Physics and Astronomy (United States); Brown, Jared M., E-mail: jared.brown@ucdenver.edu [The University of Colorado Anschutz Medical Campus, Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences (United States)

    2015-07-15

    Due to increased use of silver nanoparticles (AgNPs) for their antimicrobial activity, concerns have risen regarding potential adverse human health effects. Scavenger receptor B1 (SR-B1), a major receptor for high-density lipoprotein (HDL), is expressed by macrophages and has also been reported to play a role in recognition of negatively charged particles. We, therefore, hypothesized that SR-B1 mediates macrophage uptake of AgNPs and inflammatory activation. To test this hypothesis, we exposed a mouse macrophage cell line RAW264.7 (RAW) and bone marrow-derived macrophages (BMDM) to 20 nm citrate-suspended AgNPs. To verify the role of the SR-B1 receptor, we utilized a SR-B1 inhibitor (Blt2). In vitro studies demonstrated uptake of AgNPs and HDL-coated AgNPs by macrophages which were significantly reduced following pretreatment with Blt2. Inflammatory cytokine arrays revealed that macrophages exposed to AgNPs up-regulated expression of Tnf-α, Oncostatin m (OSM), Ccl4, Il17f, Ccl7, and Ccl2, whereas Il16 was found to be down-regulated. Macrophage activation was observed following AgNP and HDL-coated AgNP exposure as measured by OSM protein production and increased surface expression of CD86. These markers of activation were reduced with Blt2 pretreatment. The in vitro findings were confirmed in vivo through pulmonary instillation of AgNPs in mice. Pulmonary instillation of AgNPs resulted in a recruitment of inflammatory cells that were reduced in SR-B1-deficient mice or following Blt2 pretreatment. This study suggests that SR-B1 plays a major role in cellular recognition of AgNPs and the induction of cell responses that could contribute to inflammation caused by AgNP exposure.

  15. Germline recombination in a novel Cre transgenic line, Prl3b1-Cre mouse.

    Science.gov (United States)

    Al-Soudy, Al-Sayed; Nakanishi, Tsuyoshi; Mizuno, Seiya; Hasegawa, Yoshikazu; Shawki, Hossam H; Katoh, Megumi C; Basha, Walaa A; Ibrahim, Abdelaziz E; El-Shemy, Hany A; Iseki, Hiroyoshi; Yoshiki, Atsushi; Hiromori, Youhei; Nagase, Hisamitsu; Takahashi, Satoru; Oishi, Hisashi; Sugiyama, Fumihiro

    2016-07-01

    Spermatogenesis is a complex and highly regulated process by which spermatogonial stem cells differentiate into spermatozoa. To better understand the molecular mechanisms of the process, the Cre/loxP system has been widely utilized for conditional gene knockout in mice. In this study, we generated a transgenic mouse line that expresses Cre recombinase under the control of the 2.5 kbp of the Prolactin family 3, subfamily b, member 1 (Prl3b1) gene promoter (Prl3b1-cre). Prl3b1 was initially reported to code for placental lactogen 2 (PL-2) protein in placenta along with increased expression toward the end of pregnancy. PL-2 was found to be expressed in germ cells in the testis, especially in spermatocytes. To analyze the specificity and efficiency of Cre recombinase activity in Prl3b1-cre mice, the mice were mated with reporter R26GRR mice, which express GFP ubiquitously before and tdsRed exclusively after Cre recombination. The systemic examination of Prl3b1-cre;R26GRR mice revealed that tdsRed-positive cells were detected only in the testis and epididymis. Fluorescence imaging of Prl3b1-cre;R26GRR testes suggested that Cre-mediated recombination took place in the germ cells with approximately 74% efficiency determined by in vitro fertilization. In conclusion, our results suggest that the Prl3b1-cre mice line provides a unique resource to understand testicular germ-cell development. genesis 54:389-397, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  16. Activation of the kinin B1 receptor attenuates melanoma tumor growth and metastasis.

    Directory of Open Access Journals (Sweden)

    Patricia Dillenburg-Pilla

    Full Text Available Melanoma is a very aggressive tumor that does not respond well to standard therapeutic approaches, such as radio- and chemotherapies. Furthermore, acquiring the ability to metastasize in melanoma and many other tumor types is directly related to incurable disease. The B1 kinin receptor participates in a variety of cancer-related pathophysiological events, such as inflammation and angiogenesis. Therefore, we investigated whether this G protein-coupled receptor plays a role in tumor progression. We used a murine melanoma cell line that expresses the kinin B1 receptor and does not express the kinin B2 receptor to investigate the precise contribution of activation of the B1 receptor in tumor progression and correlated events using various in vitro and in vivo approaches. Activation of the kinin B1 receptor in the absence of B2 receptor inhibits cell migration in vitro and decreases tumor formation in vivo. Moreover, tumors formed from cells stimulated with B1-specific agonist showed several features of decreased aggressiveness, such as smaller size and infiltration of inflammatory cells within the tumor area, higher levels of pro-inflammatory cytokines implicated in the host anti-tumor immune response, lower number of cells undergoing mitosis, a poorer vascular network, no signs of invasion of surrounding tissues or metastasis and increased animal survival. Our findings reveal that activation of the kinin B1 receptor has a host protective role during murine melanoma tumor progression, suggesting that the B1 receptor could be a new anti-tumor GPCR and provide new opportunities for therapeutic targeting.

  17. EphrinB1 expression is dysregulated and promotes oncogenic signaling in medulloblastoma.

    Science.gov (United States)

    McKinney, Nicole; Yuan, Liangping; Zhang, Hongying; Liu, Jingbo; Cho, Yoon-Jae; Rushing, Elisabeth; Schniederjan, Matthew; MacDonald, Tobey J

    2015-01-01

    Eph receptors and ephrin ligands are master regulators of oncogenic signaling required for proliferation, migration, and metastasis. Yet, Eph/ephrin expression and activity in medulloblastoma (MB), the most common malignant brain tumor of childhood, remains poorly defined. We hypothesized that Eph/ephrins are differentially expressed by sonic hedgehog (SHH) and non-SHH MB and that specific members contribute to the aggressive phenotype. Affymetrix gene expression profiling of 29 childhood MB, separated into SHH (N = 11) and non-SHH (N = 18), was performed followed by protein validation of selected Eph/ephrins in another 60 MB and two MB cell lines (DAOY, D556). Functional assays were performed using MB cells overexpressing or deleted for selected ephrins. We found EPHB4 and EFNA4 almost exclusively expressed by SHH MB, whereas EPHA2, EPHA8, EFNA1 and EFNA3 are predominantly expressed by non-SHH MB. The remaining family members, except EFNB1, are ubiquitously expressed by over 70-90 % MB, irrespective of subgroup. EFNB1 is the only member differentially expressed by 28 % of SHH and non-SHH MB. Corresponding protein expression for EphB/ephrinB1 and B2 was validated in MB. Only ephrinB2 was also detected in fetal cerebellum, indicating that EphB/ephrinB1 expression is MB-specific. EphrinB1 immunopositivity localizes to tumor cells within MB with the highest proliferative index. EphrinB1 overexpression promotes EphB activation, alters F-actin distribution and morphology, decreases adhesion, and significantly promotes proliferation. Either silencing or overexpression of ephrinB1 impairs migration. These results indicate that EphrinB1 is uniquely dysregulated in MB and promotes oncogenic responses in MB cells, implicating ephrinB1 as a potential target.

  18. Anglizismen im berufsbezogenen DaF-Unterricht – Die Untersuchung von Anglizismen im Bereich der Luftfahrt

    Directory of Open Access Journals (Sweden)

    Sara Orthaber

    2015-11-01

    Full Text Available Fremdsprachenkenntnisse sind heutzutage erforderlich, insbesondere in einem Land wie Slowenien. Nicht nur aufgrund dessen, dass man mit der slowenischen Sprache geographisch begrenzt ist, sondern da auch in der Mehrzahl der ausgeschriebenen Stellen Fremdsprachenkenntnisse erwünscht sind. Aus diesem Grund werden auch im tertiären Bildungssektor mindestens zwei berufsbezogene Fremdsprachen angeboten, von denen die Studenten eine wählen müssen. Und obwohl sich die Meisten für Englisch entscheiden, ist in den letzten Jahren ein zunehmendes Interesse an Deutschkursen zu verzeichnen. Ungeachtet dessen ist heutzutage ein gewisses Fundament an Englischkenntnissen praktisch unerlässlich, was auch beim Unterrichten des Faches Deutsch als Fremdsprache aufgefallen ist, wo in fast allen Kommunikationssituationen der Arbeitswelt, insbesondere aber in der Fachterminologie, ein zunehmender Einfluss der englischen auf die deutsche Sprache festzustellen ist. In diesem Beitrag wird der Gebrauch von Anglizismen in Lehrmaterialien zum Thema Luftfahrt untersucht, die an der Fakultät für Logistik beim DaF-Unterricht verwendet werden. Zu diesem Zweck werden zuerst die für den Unterricht relevanten Anglizismen aufgeführt und diskutiert. Im Anschluss erfolgen Implikationen für das Lehren und Erlernen der berufsbezogenen Fremdsprache.

  19. B-1 cells modulate the murine macrophage response to Leishmania major infection.

    Science.gov (United States)

    Arcanjo, Angelica F; Nunes, Marise P; Silva-Junior, Elias B; Leandro, Monique; da Rocha, Juliana Dutra Barbosa; Morrot, Alexandre; Decote-Ricardo, Debora; Freire-de-Lima, Celio Geraldo

    2017-05-26

    To investigate the modulatory effect of B-1 cells on murine peritoneal macrophages infected with Leishmania major ( L. major ) in vitro . Peritoneal macrophages obtained from BALB/c and BALB/c XID mice were infected with L. major and cultured in the presence or absence of B-1 cells obtained from wild-type BALB/c mice. Intracellular amastigotes were counted, and interleukin-10 (IL-10) production was quantified in the cellular supernatants using an enzyme-linked immunosorbent assay. The levels of the lipid mediator prostaglandin E2 (PGE 2 ) were determined using a PGE 2 enzyme immunoassay kit (Cayman Chemical, Ann Arbor, MI), and the number of lipid bodies was quantified in the cytoplasm of infected macrophages in the presence and absence of B-1 cells. Culturing the cells with selective PGE 2 -neutralizing drugs inhibited PGE 2 production and confirmed the role of this lipid mediator in IL-10 production. In contrast, we demonstrated that B-1 cells derived from IL-10 KO mice did not favor the intracellular growth of L. major . We report that B-1 cells promote the growth of L. major amastigotes inside peritoneal murine macrophages. We demonstrated that the modulatory effect was independent of physical contact between the cells, suggesting that soluble factor(s) were released into the cultures. We demonstrated in our co-culture system that B-1 cells trigger IL-10 production by L. major -infected macrophages. Furthermore, the increased secretion of IL-10 was attributed to the presence of the lipid mediator PGE 2 in supernatants of L. major -infected macrophages. The presence of B-1 cells also favors the production of lipid bodies by infected macrophages. In contrast, we failed to obtain the same effect on parasite replication inside L. major -infected macrophages when the B-1 cells were isolated from IL-10 knockout mice. Our results show that elevated levels of PGE 2 and IL-10 produced by B-1 cells increase L. major growth, as indicated by the number of parasites in cell

  20. Prenatal exposure to aflatoxin B1: developmental, behavioral, and reproductive alterations in male rats

    Science.gov (United States)

    Supriya, Ch.; Reddy, P. Sreenivasula

    2015-06-01

    Previous studies have shown that aflatoxin B1 (AfB1) inhibits androgen biosynthesis as a result of its ability to form a high-affinity complex with the steroidogenic acute regulatory protein. The results of the present study demonstrate the postnatal effects of in utero exposure to AfB1 in the rat. Pregnant Wistar rats were given 10, 20, or 50 μg AfB1/kg body weight daily from gestation day (GD) 12 to GD 19. At parturition, newborns were observed for clinical signs and survival. All animals were born alive and initially appeared to be active. Male pups from control and AfB1-exposed animals were weaned and maintained up to postnatal day (PD) 100. Litter size, birth weight, sex ratio, survival rate, and crown-rump length of the pups were significantly decreased in AfB1-exposed rats when compared to controls. Elapsed time (days) for testes to descend into the scrotal sac was significantly delayed in experimental pups when compared to control pups. Behavioral observations such as cliff avoidance, negative geotaxis, surface rightening activity, ascending wire mesh, open field behavior, and exploratory and locomotory activities were significantly impaired in experimental pups. Body weights and the indices of testis, cauda epididymis, prostate, seminal vesicles, and liver were significantly reduced on PD 100 in male rats exposed to AfB1 during embryonic development when compared with controls. Significant reduction in the testicular daily sperm production, epididymal sperm count, and number of viable, motile, and hypo-osmotic tail coiled sperm was observed in experimental rats. The levels of serum testosterone and activity levels of testicular hydroxysteroid dehydrogenases were significantly decreased in a dose-dependent manner with a significant increase in the serum follicle-stimulating hormone and luteinizing hormone in experimental rats. Deterioration in the testicular and cauda epididymal architecture was observed in experimental rats. The results of fertility

  1. Volumetric B1 (+) mapping of the brain at 7T using DREAM.

    Science.gov (United States)

    Nehrke, Kay; Versluis, Maarten J; Webb, Andrew; Börnert, Peter

    2014-01-01

    To tailor and optimize the Dual Refocusing Echo Acquisition Mode (DREAM) approach for volumetric B1 (+) mapping of the brain at 7T. A new DREAM echo timing scheme based on the virtual stimulated echo was derived to minimize potential effects of transverse relaxation. Furthermore, the DREAM B1 (+) mapping performance was investigated in simulations and experimentally in phantoms and volunteers for volumetric applications, studying and optimizing the accuracy of the sequence with respect to saturation effects, slice profile imperfections, and T1 and T2 relaxation. Volumetric brain protocols were compiled for different isotropic resolutions (5-2.5 mm) and SENSE factors, and were studied in vivo for different RF drive modes (circular/linear polarization) and the application of dielectric pads. Volumetric B1 (+) maps with good SNR at 2.5 mm isotropic resolution were acquired in about 20 s or less. The specific absorption rate was well below the safety limits for all scans. Mild flow artefacts were observed in the large vessels. Moreover, a slight contrast in the ventricle was observed in the B1 (+) maps, which could be attributed to T1 and T2 relaxation effects. DREAM enables safe, very fast, and robust volumetric B1 (+) mapping of the brain at ultrahigh fields. Copyright © 2013 Wiley Periodicals, Inc.

  2. Multi-turn transmit coil to increase b1 efficiency in current source amplification.

    Science.gov (United States)

    Gudino, N; Griswold, M A

    2013-04-01

    A multi-turn transmit surface coil design was presented to improve B1 efficiency when used with current source amplification. Three different coil designs driven by an on-coil current-mode class-D amplifier with current envelope feedback were tested on the benchtop and through imaging in a 1.5 T scanner. Case temperature of the power field-effect transistor at the amplifier output stage was measured to evaluate heat dissipation for the different current levels and coil configurations. In addition, a lower power rated device was tested to exploit the potential gain in B1 obtained with the multi-turn coil. As shown both on the benchtop and in a 1.5 T scanner, B1 was increased by almost 3-fold without increasing heat dissipation on the power device at the amplifier's output using a multi-turn surface coil. Similar gain was obtained when connecting a lower power rated field-effect transistor to the multi-turn coil. In addition to reduce heat dissipation per B1 in the device, higher B1 per current efficiency allows the use of field-effect transistors with lower current ratings and lower port capacitances, which could improve the overall performance of the on-coil current source transmit system. Copyright © 2013 Wiley Periodicals, Inc.

  3. Encoding methods for B1+ mapping in parallel transmit systems at ultra high field

    Science.gov (United States)

    Tse, Desmond H. Y.; Poole, Michael S.; Magill, Arthur W.; Felder, Jörg; Brenner, Daniel; Jon Shah, N.

    2014-08-01

    Parallel radiofrequency (RF) transmission, either in the form of RF shimming or pulse design, has been proposed as a solution to the B1+ inhomogeneity problem in ultra high field magnetic resonance imaging. As a prerequisite, accurate B1+ maps from each of the available transmit channels are required. In this work, four different encoding methods for B1+ mapping, namely 1-channel-on, all-channels-on-except-1, all-channels-on-1-inverted and Fourier phase encoding, were evaluated using dual refocusing acquisition mode (DREAM) at 9.4 T. Fourier phase encoding was demonstrated in both phantom and in vivo to be the least susceptible to artefacts caused by destructive RF interference at 9.4 T. Unlike the other two interferometric encoding schemes, Fourier phase encoding showed negligible dependency on the initial RF phase setting and therefore no prior B1+ knowledge is required. Fourier phase encoding also provides a flexible way to increase the number of measurements to increase SNR, and to allow further reduction of artefacts by weighted decoding. These advantages of Fourier phase encoding suggest that it is a good choice for B1+ mapping in parallel transmit systems at ultra high field.

  4. Validation of the IMS CORE Diabetes Model.

    Science.gov (United States)

    McEwan, Phil; Foos, Volker; Palmer, James L; Lamotte, Mark; Lloyd, Adam; Grant, David

    2014-09-01

    The IMS CORE Diabetes Model (CDM) is a widely published and validated simulation model applied in both type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) analyses. Validation to external studies is an important part of demonstrating model credibility. Because the CDM is widely used to estimate long-term clinical outcomes in diabetes patients, the objective of this analysis was to validate the CDM to contemporary outcomes studies, including those with long-term follow-up periods. A total of 112 validation simulations were performed, stratified by study follow-up duration. For long-term results (≥15-year follow-up), simulation cohorts representing baseline Diabetes Control and Complications Trial (DCCT) and United Kingdom Prospective Diabetes Study (UKPDS) cohorts were generated and intensive and conventional treatment arms were defined in the CDM. Predicted versus observed macrovascular and microvascular complications and all-cause mortality were assessed using the coefficient of determination (R(2)) goodness-of-fit measure. Across all validation studies, the CDM simulations produced an R(2) statistic of 0.90. For validation studies with a follow-up duration of less than 15 years, R(2) values of 0.90 and 0.88 were achieved for T1DM and T2DM respectively. In T1DM, validating against 30-year outcomes data (DCCT) resulted in an R(2) of 0.72. In T2DM, validating against 20-year outcomes data (UKPDS) resulted in an R(2) of 0.92. This analysis supports the CDM as a credible tool for predicting the absolute number of clinical events in DCCT- and UKPDS-like populations. With increasing incidence of diabetes worldwide, the CDM is particularly important for health care decision makers, for whom the robust evaluation of health care policies is essential. Copyright © 2014 International Society for Pharmacoeconomics and Outcomes Research (ISPOR). Published by Elsevier Inc. All rights reserved.

  5. Grundlegende Steuerungsverfahren im heterogenen Logistiknetz mit Kanban

    Science.gov (United States)

    Dickmann, Eva; Dickmann, Philipp; Lödding, Hermann; Möller, Niklas; Rücker, Thomas; Schneider, Herfried M.; Zäh, Michael F.

    In vielen Unternehmen werden heterogene (verschiedene) Steuerungen in einem abgestimmten Konzept kombiniert. Je nach Anwendungsfall und Rahmenbedingungen werden Kombinationen allgemein bekannter Steuerungen oder Steuerungsvarianten gemischt eingesetzt, um eine optimale Steuerung für unterschiedliche Fälle zu erreichen. Hierbei stehen neben den bekannten und weit verbreiteten Methoden, wie Material Requirements Planning (MRP) oder Kanban, auch weniger bekannte oder neue Methoden zur Auswahl, wie die Produktionssteuerung mit dezentraler, bestandsorientierter Fertigungsregelung (DBF). Kanban ist ein simples und effizientes Steuerungskonzept, das in der klassischen Form für spezifische einfache Anwendungsfälle umsetzbar ist. Hochentwickelte Steuerungsalgorithmen können helfen, komplexe Abläufe optimal abzubilden. Mit einer grundlegenden Vereinfachung der Abläufe kann allerdings in vielen Fällen ein wesentlich stärkerer und umfassender Verbesserungseffekt erzielt werden. Die wesentliche Fragestellung sollte folglich lauten: Warum ist der Ablauf nicht mit einer einfachen Steuerung wie Kanban abzubilden? Um die Vorteile des Konzepts auch in untypischen Bereichen anwenden zu können, sind jedoch verschiedene Varianten oder Kanban-ähnliche Steuerungsmethoden entstanden. Darüber hinaus sind in der Praxis hybride Steuerungen im Einsatz, welche so kombiniert werden, dass die Zusammensetzung anspruchsvolle Eigenschaftsbilder noch exakt abbildet. In der Praxis basieren die Steuerungsentscheidungen nur zu einem kleinen Teil auf den eigentlichen Steuerungsalgorithmen, wie sie uns das MRP-System zur Verfügung stellt. Moderne Steuerungswelten" schließen alle relevanten Informationsquellen in eine heterogene Entscheidungsmatrix mit ein. Letztlich zählt nicht, ob die Entscheidung auf den Informationen aus dem MRP-System oder auf Softfacts basierend getroffen wurde, sondern nur, ob die Entscheidung erfolgreich war.

  6. Measurement of the Tensor Structure Function b1 of the Deuteron

    Science.gov (United States)

    Airapetian, A.; Akopov, N.; Akopov, Z.; Amarian, M.; Ammosov, V. V.; Andrus, A.; Aschenauer, E. C.; Augustyniak, W.; Avakian, R.; Avetissian, A.; Avetissian, E.; Bailey, P.; Balin, D.; Baturin, V.; Beckmann, M.; Belostotski, S.; Bernreuther, S.; Bianchi, N.; Blok, H. P.; Böttcher, H.; Borissov, A.; Borysenko, A.; Bouwhuis, M.; Brack, J.; Brüll, A.; Bryzgalov, V.; Capitani, G. P.; Chen, T.; Chiang, H. C.; Ciullo, G.; Contalbrigo, M.; Dalpiaz, P. F.; Leo, R. De; Demey, M.; Nardo, L. De; Sanctis, E. De; Devitsin, E.; Nezza, P. Di; Dreschler, J.; Düren, M.; Ehrenfried, M.; Elalaoui-Moulay, A.; Elbakian, G.; Ellinghaus, F.; Elschenbroich, U.; Fabbri, R.; Fantoni, A.; Fechtchenko, A.; Felawka, L.; Fox, B.; Frullani, S.; Gapienko, G.; Gapienko, V.; Garibaldi, F.; Garrow, K.; Garutti, E.; Gaskell, D.; Gavrilov, G.; Gharibyan, V.; Graw, G.; Grebeniouk, O.; Greeniaus, L. G.; Gregor, I. M.; Hafidi, K.; Hartig, M.; Hasch, D.; Heesbeen, D.; Henoch, M.; Hertenberger, R.; Hesselink, W. H. A.; Hillenbrand, A.; Hoek, M.; Holler, Y.; Hommez, B.; Iarygin, G.; Ivanilov, A.; Izotov, A.; Jackson, H. E.; Jgoun, A.; Kaiser, R.; Kinney, E.; Kisselev, A.; Kopytin, M.; Korotkov, V.; Kozlov, V.; Krauss, B.; Krivokhijine, V. G.; Lagamba, L.; Lapikás, L.; Laziev, A.; Lenisa, P.; Liebing, P.; Linden-Levy, L. A.; Lipka, K.; Lorenzon, W.; Lu, H.; Lu, J.; Lu, S.; Ma, B.-Q.; Maiheu, B.; Makins, N. C. R.; Mao, Y.; Marianski, B.; Marukyan, H.; Masoli, F.; Mexner, V.; Meyners, N.; Mikloukho, O.; Miller, C. A.; Miyachi, Y.; Muccifora, V.; Nagaitsev, A.; Nappi, E.; Naryshkin, Y.; Nass, A.; Negodaev, M.; Nowak, W.-D.; Oganessyan, K.; Ohsuga, H.; Pickert, N.; Potashov, S.; Potterveld, D. H.; Raithel, M.; Reggiani, D.; Reimer, P. E.; Reischl, A.; Reolon, A. R.; Riedl, C.; Rith, K.; Rosner, G.; Rostomyan, A.; Rubacek, L.; Rubin, J.; Ryckbosch, D.; Salomatin, Y.; Sanjiev, I.; Savin, I.; Schäfer, A.; Schill, C.; Schnell, G.; Schüler, K. P.; Seele, J.; Seidl, R.; Seitz, B.; Shanidze, R.; Shearer, C.; Shibata, T.-A.; Shutov, V.; Simani, M. C.; Sinram, K.; Stancari, M.; Statera, M.; Steffens, E.; Steijger, J. J. M.; Stenzel, H.; Stewart, J.; Stinzing, F.; Stösslein, U.; Tait, P.; Tanaka, H.; Taroian, S.; Tchuiko, B.; Terkulov, A.; Tkabladze, A.; Trzcinski, A.; Tytgat, M.; Vandenbroucke, A.; van der Nat, P. B.; van der Steenhoven, G.; Vetterli, M. C.; Vikhrov, V.; Vincter, M. G.; Vogel, C.; Vogt, M.; Volmer, J.; Weiskopf, C.; Wendland, J.; Wilbert, J.; Ye, Y.; Ye, Z.; Yen, S.; Zihlmann, B.; Zupranski, P.

    2005-12-01

    The Hermes experiment has investigated the tensor spin structure of the deuteron using the 27.6GeV/c positron beam of DESY HERA. The use of a tensor-polarized deuteron gas target with only a negligible residual vector polarization enabled the first measurement of the tensor asymmetry Azzd and the tensor structure function b1d for average values of the Bjorken variable 0.01<⟨x⟩<0.45 and of the negative of the squared four-momentum transfer 0.5GeV2<⟨Q2⟩<5GeV2. The quantities Azzd and b1d are found to be nonzero. The rise of b1d for decreasing values of x can be interpreted to originate from the same mechanism that leads to nuclear shadowing in unpolarized scattering.

  7. The structure and the analytical potential energy function of NH2 (X2B1)

    International Nuclear Information System (INIS)

    Liu Yufang; Jiang Lijuan; Ma Heng; Sun Jinfeng

    2008-01-01

    This paper reports that the equilibrium structure of NH 2 has been optimized at the QCISD/6-311++G (3df, 3pd) level. The ground-state NH 2 has a bent (C 2v , X 2 B 1 ) structure with an angle of 103.0582°. The geometrical structure is in good agreement with the other calculational and experimental results. The harmonic frequencies and the force constants have also been calculated. Based on the group theory and the principle of microscopic reversibility, the dissociation limits of NH 2 (C 2v , X 2 B 1 ) have been derived. The potential energy surface of NH 2 (X 2 B 1 ) is reasonable. The contour lines are constructed, the structure and energy of NH 2 reappear on the potential energy surface

  8. Production of aflatexin B1 in wheat grains under different environmental storage conditions

    International Nuclear Information System (INIS)

    Mahrous, S.R.; Shahin, A.A.M.; Bothaina, M.Y.

    2000-01-01

    Fungal flora of stored wheat grains and the production of aflatoxin B 1 by Aflavus in wheat grains under different environmental conditions were examined. Aspergillus, Penicillium,. Fusarium, Cladosporium, Curvularia, Epicouccum, Verticilium, Rhizopus, Mucor and Altenaria were the predominant fungi isolated from the collected non-disinfected grains. Aspergillus spp, were only isolated from surface disinfected grains. Of 223 aspergillus spp, isolates only 128 found to aflatoxin producing and all aflatoxin producing-fungi belonged to the Aflavus group. Results demonstrate that Aflavus could produce maximum concentration of aflatoxin B 1 in grains at 20% moisture (163.5 MOU g/kg). The highest concentration of aflatoxin B 1 was produced by Aflavus (10 5 spores/g) in wheat grains with 20% moisture after 20 days at 30 degree and 92.40 % R.H. The aflatoxin production did not increase monotonously as a function of inoculum density

  9. Comparative analysis between P1 and B1 equations for neutron moderation

    International Nuclear Information System (INIS)

    Martinez, Aquilino Senra; Silva, Fernando Carvalho da; Cardoso, Carlos Eduardo Santos

    2000-01-01

    In order to calculate the neutron flux in nuclear reactors, B1 or P1 equations are solved by numerical methods for several groups of energy. The neutron fluxes obtained from the solutions of the B1 and P1 equations are similar when they are applied to large nuclear power reactors. However, an important difference between the two fluxes is that the system of P1 equations uses one more approximation than the B1 system and then, its flux is less precise. The present work shows the relations between both equations and analyzes for what conditions the two equations systems are equivalent. Furthermore, this equations are numerically solved in 54 groups of energy for a quadrangular arrange. (author)

  10. Prevalence of Virulent Escherichia coli Belonging B1 Phylogroup in Municipal Water Supply in Dhaka, Bangladesh

    DEFF Research Database (Denmark)

    Ferdous, Jannataul; Rashid, Ridwan Bin; Tulsiani, Suhella

    isolated from drinking water in Arichpur, a low income area of Dhaka, Bangladesh. The distribution of the phylogroups and virulence genes were investigated in 200 isolates among them 110 isolates were from municipal water supply system and 90 were from household drinking water. Gene profile of virulence.......001. Therefore, it can be inferred municipal water supply was a greater contributor of pathogenic E. coli from the B1 phylogroup. Usually commensals fall in the Phylogroups A and B1. The presence of greater number of virulent B1 phylogroup isolates originating from municipal water supply indicates......Escherichia coli is a commensal organism of the digestive tracts of many vertebrates, including humans. Contamination of drinking water with pathogenic E. coli is a serious public health concern. This study focused on the distribution of phylogenetic groups and virulence gene profile of E. coli...

  11. L1157-B1: WATER AND AMMONIA AS DIAGNOSTICS OF SHOCK TEMPERATURE

    International Nuclear Information System (INIS)

    Viti, S.; Yates, J. A.; Jimenez-Serra, I.; Codella, C.; Vasta, M.; Caselli, P.; Lefloch, B.; Ceccarelli, C.

    2011-01-01

    We investigate the origin and nature of the profiles of water and ammonia observed toward the L1157-B1 clump as part of the HIFI CHESS survey using a new code coupling a gas-grain chemical model with a parametric shock model. First results from the unbiased survey reveal different molecular components at different excitation conditions coexisting in the B1 bow shock structure, with NH 3 , H 2 CO, and CH 3 OH emitting only at relatively low outflow velocities whereas H 2 O shows bright emission at high velocities. Our model suggests that these differences are purely chemical and can be explained by the presence of a C-type shock whose maximum temperature must be close to 4000 K along the B1 clump.

  12. Ion mobility spectrometer / mass spectrometer (IMS-MS).

    Energy Technology Data Exchange (ETDEWEB)

    Hunka Deborah Elaine; Austin, Daniel E.

    2005-07-01

    The use of Ion Mobility Spectrometry (IMS) in the Detection of Contraband Sandia researchers use ion mobility spectrometers for trace chemical detection and analysis in a variety of projects and applications. Products developed in recent years based on IMS-technology include explosives detection personnel portals, the Material Area Access (MAA) checkpoint of the future, an explosives detection vehicle portal, hand-held detection systems such as the Hound and Hound II (all 6400), micro-IMS sensors (1700), ordnance detection (2500), and Fourier Transform IMS technology (8700). The emphasis to date has been on explosives detection, but the detection of chemical agents has also been pursued (8100 and 6400). Combining Ion Mobility Spectrometry (IMS) with Mass Spectrometry (MS) is described. The IMS-MS combination overcomes several limitations present in simple IMS systems. Ion mobility alone is insufficient to identify an unknown chemical agent. Collision cross section, upon which mobility is based, is not sufficiently unique or predictable a priori to be able to make a confident peak assignment unless the compounds present are already identified. Molecular mass, on the other hand, is much more readily interpreted and related to compounds. For a given compound, the molecular mass can be determined using a pocket calculator (or in one's head) while a reasonable value of the cross-section might require hours of computation time. Thus a mass spectrum provides chemical specificity and identity not accessible in the mobility spectrum alone. In addition, several advanced mass spectrometric methods, such as tandem MS, have been extensively developed for the purpose of molecular identification. With an appropriate mass spectrometer connected to an ion mobility spectrometer, these advanced identification methods become available, providing greater characterization capability.

  13. Instrumentale Spielformeln und Vokale Verzierungen im 16.Jahrhundert

    Directory of Open Access Journals (Sweden)

    Göllner, Theodor

    2001-12-01

    Full Text Available In the music of the 16th century, stereotype four-note formulas are found in the instrumental as well as in the vocal idiom. Although frequently identical in appearance, their functions are very different. While indispensable in the instrumental idiom, particularly in keyboard music, where they represent the very essence of instruction in 15th-century organ treatises and fundamenta, they appear in vocal music only as additions to finished compositions or existing practices. What amounts to an absolute necessity in one case is merely an ornamental supplement in the other. In vocal music it is treated as a matter of performance carried out by a qualified soloist and limited to the musical centers of Italy. The present article raises the question as to how the genuinely instrumental and widely spread keyboard practice made its impact on the vocal ornaments of the singer, whose primary concern is the musical communication of language.

    [de] Die in der Musik des 16. Jahrhunderts im Instrumentalen wie im Vokalen vorkommenden, meist viertönigen, stereotypen Formeln sind zwar in beiden Bereichen weitgehend identisch, doch von höchst unterschiedlicher Funktion. Während sie im Instrumentalen, besonders in der Tastenmusik, unverzichtbar sind und schon im 15. Jahrhundert den Kern der Orgeltraktate und Fundamenta bilden, treten sie im Vokalen zu einer fertigen Komposition oder bestehenden Praxis hinzu. Was im einen Fall eine unbedingte Notwendigkeit ist, wird im anderen zu einem nachträglichen Ornament, das sich oft erst bei der Aufftührung einstellt, den qualifizierten Solosänger voraussetzt und auf die musikalischen Zentren Italiens konzentriert war. Der Artikel versucht zu klären, wie sich das genuin instrumentale, allgemein verbreitete Tastenspiel auf die spezielle Verzierungskunst des Sängers auswirkt, dessen primäre Aufgabe es ist, Sprache musikalisch zu vermitteln.

  14. A New IMS Based Inter-working Solution

    Science.gov (United States)

    Zhu, Zhongwen; Brunner, Richard

    With the evolution of third generation network, more and more multimedia services are developed and deployed. Any new service to be deployed in IMS network is required to inter-work with existing Internet communities or legacy terminal users in order to appreciate the end users, who are the main drivers for the service to succeed. The challenge for Inter-working between IMS (IP Multimedia Subsystem) and non-IMS network is “how to handle recipient’s address”. This is because each network has its own routable address schema. For instance, the address for Google Talk user is xmpp:xyz@google.com, which is un-routable in IMS network. Hereafter a new Inter-working (IW) solution between IMS and non-IMS network is proposed for multimedia services that include Instant Messaging, Chat, and File transfer, etc. It is an end-to-end solution built on IMS infrastructure. The Public Service Identity (PSI) defined in 3GPP standard (3rd Generation Partnership Project) is used to allow terminal clients to allocate this IW service. When sending the SIP (Session Initial Protocol) request out for multimedia services, the terminal includes the recipient’s address in the payload instead of the “Request-URI” header. In the network, the proposed solution provides the mapping rules between different networks in MM-IW (Multimedia IW). The detailed technical description and the corresponding use cases are present. The comparison with other alternatives is made. The benefits of the proposed solution are highlighted.

  15. Patterns of cyclin A and B1 immunostaining in papillary thyroid carcinoma.

    Science.gov (United States)

    Cyniak-Magierska, Anna; Stasiak, Magdalena; Naze, Maciej; Dedecjus, Marek; Brzeziński, Jan; Lewiński, Andrzej

    2015-01-01

    Cyclin A, encoded by CCNA (cyclin A) gene with locus in chromosome 4q27, and cyclin B1, encoded by CCNB1 (cyclin B1) gene with locus in chromosome 5q12, are proteins that play a key role in the passage through the restriction point in G2 phase of the cell cycle. The aim of the study was to analyse immunohistochemically the expression of cyclins A and B1 in different variants of papillary thyroid carcinoma (PTC). The immunostaining patterns of the proteins in question in the tissue of 40 resected PTC (20 cases of classic variant of PTC, 9 cases of PTC follicular variant and 11 cases of other non-classic variants of PTC) were investigated. On analyzing cyclin A and B1 expression, positive staining in 90% cases of PTC were observed. The study revealed a significant difference in expression of cyclins A and B1 between classic and non-classic variants of PTC. The expression of both examined cyclins was weaker in the classic variant of PTC. In the group of follicular variant of PTC, the expression of cyclins was of medium intensity and in the group of other non-classic variants of PTC, the expression was clearly higher. The results of the presented study suggest that cyclins A and B1 expression may have a characteristic pattern of immunostaining for particular variants of PTC. If the obtained results are confirmed in a larger group of patients, the diagnostic panel constructed of the antibodies against these proteins may increase the diagnostic accuracy in PTC cases.

  16. Cdc2/cyclin B1 regulates centrosomal Nlp proteolysis and subcellular localization.

    Science.gov (United States)

    Zhao, Xuelian; Jin, Shunqian; Song, Yongmei; Zhan, Qimin

    2010-11-01

    The formation of proper mitotic spindles is required for appropriate chromosome segregation during cell division. Aberrant spindle formation often causes aneuploidy and results in tumorigenesis. However, the underlying mechanism of regulating spindle formation and chromosome separation remains to be further defined. Centrosomal Nlp (ninein-like protein) is a recently characterized BRCA1-regulated centrosomal protein and plays an important role in centrosome maturation and spindle formation. In this study, we show that Nlp can be phosphorylated by cell cycle protein kinase Cdc2/cyclin B1. The phosphorylation sites of Nlp are mapped at Ser185 and Ser589. Interestingly, the Cdc2/cyclin B1 phosphorylation site Ser185 of Nlp is required for its recognition by PLK1, which enable Nlp depart from centrosomes to allow the establishment of a mitotic scaffold at the onset of mitosis . PLK1 fails to dissociate the Nlp mutant lacking Ser185 from centrosome, suggesting that Cdc2/cyclin B1 might serve as a primary kinase of PLK1 in regulating Nlp subcellular localization. However, the phosphorylation at the site Ser589 by Cdc2/cyclin B1 plays an important role in Nlp protein stability probably due to its effect on protein degradation. Furthermore, we show that deregulated expression or subcellular localization of Nlp lead to multinuclei in cells, indicating that scheduled levels of Nlp and proper subcellular localization of Nlp are critical for successful completion of normal cell mitosis, These findings demonstrate that Cdc2/cyclin B1 is a key regulator in maintaining appropriate degradation and subcellular localization of Nlp, providing novel insights into understanding on the role of Cdc2/cyclin B1 in mitotic progression.

  17. Participação de linfócitos B-1 na criptococose experimental

    OpenAIRE

    Eliver Eid Bou Ghosn

    2004-01-01

    A criptococose é uma infecção fúngica causada pela levedura encapsulada Cryptococcus neoformans que acomete freqüentemente o meningoencéfalo de pacientes imunocomprometidos. No Brasil, cerca de 6% dos pacientes com AIDS desenvolvem a criptococose. A construção da resposta imune na criptococose envolve fagócitos e linfócitos T e B presentes no granuloma. Atualmente, são descritos três subtipos de linfócitos B: linfócitos B-1 a, B-1 b e B-2, o último conhecido como linfócito B \\"convencional\\"....

  18. A New Orbit for Comet C/1865 B1 (Great Southern Comet of 1865)

    Science.gov (United States)

    Branham, Richard L., Jr.

    2018-04-01

    Comet C/1865 B1 (Great southern comet of 1865), observed only in the southern hemisphere, is one of a large number of comets with parabolic orbits. Given that there are 202 observations in right ascension and 165 in declination it proves possible to calculate a better orbit than that Körber published in 1887, the orbit used in various catalogs and data bases. C/1865 B1's orbit is hyperbolic and statistically distinguishable from a parabola. This object, therefore, cannot be considered an NEO. The comet has a small perihelion distance of 0.026 AU.

  19. Kinin B1 receptors contributes to acute pain following minor surgery in humans

    Directory of Open Access Journals (Sweden)

    Brahim Jaime S

    2010-02-01

    Full Text Available Abstract Background Kinins play an important role in regulation of pain and hyperalgesia after tissue injury and inflammation by activating two types of G-protein-coupled receptors, the kinin B1 and B2 receptors. It is generally accepted that the B2 receptor is constitutively expressed, whereas the B1 receptor is induced in response to inflammation. However, little is known about the regulatory effects of kinin receptors on the onset of acute inflammation and inflammatory pain in humans. The present study investigated the changes in gene expression of kinin receptors and the levels of their endogenous ligands at an early time point following tissue injury and their relation to clinical pain, as well as the effect of COX-inhibition on their expression levels. Results Tissue injury resulted in a significant up-regulation in the gene expression of B1 and B2 receptors at 3 hours post-surgery, the onset of acute inflammatory pain. Interestingly, the up-regulation in the gene expression of B1 and B2 receptors was positively correlated to pain intensity only after ketorolac treatment, signifying an interaction between prostaglandins and kinins in the inflammatory pain process. Further, the gene expression of both B1 and B2 receptors were correlated. Following tissue injury, B1 ligands des-Arg9-BK and des-Arg10-KD were significantly lower at the third hour compared to the first 2 hours in both the placebo and the ketorolac treatment groups but did not differ significantly between groups. Tissue injury also resulted in the down-regulation of TRPV1 gene expression at 3 hours post-surgery with no significant effect by ketorolac treatment. Interestingly, the change in gene expression of TRPV1 was correlated to the change in gene expression of B1 receptor but not B2 receptor. Conclusions These results provide evidence at the transcriptional level in a clinical model of tissue injury that up-regulation of kinin receptors are involved in the development of the

  20. Sampling and Analysis Instruction for Borehole Sampling at 118-B-1 Burial Ground

    Energy Technology Data Exchange (ETDEWEB)

    W. S. Thompson

    2007-04-02

    The Washington Closure Hanford (WCH) Field Remediation Project has removed all of the disposed materials and contaminated soil from the 118-B-1 Burial Ground with the exception of tritium-contaminated soil that is believed to extend from the bottom of the present excavation to groundwater and is believed to contribute to tritium contamination observed at down-gradient monitoring Well 199-B8-6. This sampling and analysis instruction (SAI) provides the requirements for sample collection and laboratory analysis for characterization of the vertical distribution of tritium contamination in the vadose zone soil below the 118-B-1 Burial Ground remedial action excavation.

  1. Status of IMS workshops - CDAW 1: December 1977 events

    Science.gov (United States)

    Rostoker, G.

    In connection with the acquisition of a very comprehensive data set as a result of the International Magnetospheric Study (IMS), investigations were being conducted concerning the most appropriate approaches for an analysis of the data by IMS researchers, taking into account procedures which would ensure an exposure of the results of the IMS to the largest possible audience in the least amount of time. Two formats considered for interaction among IMS researchers include the 'Working Conference' and the prototype of the Coordinated Data Analysis Workshop (CDAW). It was recognized that the large amount of data involved demanded the use of a large computer data bank if any progress was to be made in a time-limited workshop. It was decided to discuss the nature of the data acquired over the December 1977 interval at a Working Conference in June 1978. Some time after the Working Conference it was announced that CDAW 1.0 would be held at the Satellite Situation Center in December 1978. It is pointed out that the IMS community has profited greatly from the experience of CDAW 1.

  2. Handover Based IMS Registration Scheme for Next Generation Mobile Networks

    Directory of Open Access Journals (Sweden)

    Shireen Tahira

    2017-01-01

    Full Text Available Next generation mobile networks aim to provide faster speed and more capacity along with energy efficiency to support video streaming and massive data sharing in social and communication networks. In these networks, user equipment has to register with IP Multimedia Subsystem (IMS which promises quality of service to the mobile users that frequently move across different access networks. After each handover caused due to mobility, IMS provides IPSec Security Association establishment and authentication phases. The main issue is that unnecessary reregistration after every handover results in latency and communication overhead. To tackle these issues, this paper presents a lightweight Fast IMS Mobility (FIM registration scheme that avoids unnecessary conventional registration phases such as security associations, authentication, and authorization. FIM maintains a flag to avoid deregistration and sends a subsequent message to provide necessary parameters to IMS servers after mobility. It also handles the change of IP address for user equipment and transferring the security associations from old to new servers. We have validated the performance of FIM by developing a testbed consisting of IMS servers and user equipment. The experimental results demonstrate the performance supremacy of FIM. It reduces media disruption time, number of messages, and packet loss up to 67%, 100%, and 61%, respectively, as compared to preliminaries.

  3. A SURVEY ON INDIAN EXPERIENCE ON INTEGRATED MANAGEMENT STANDARDS (IMS

    Directory of Open Access Journals (Sweden)

    H. Khanna

    2009-09-01

    Full Text Available Adoption of management systems standards is a key issue in manufacturing industry in India. Following the global trend quality and environmental issues are gaining importance. However the number of ISO 14001 certified companies are much less in India as compared to ISO 9001. The integration of ISO 14001 with ISO 9001 may help companies to sustain competitive advantage and overcome disappointments with quality standards and in turn encourage companies to adopt good environmental practices. The aim of this research is to study the implementation of integrated management standards (IMS by the manufacturing organizations in India. The different aspects of integration and benefits of IMS implementation are analyzed. This r esearch is based on empirical study carried out in Indian manufacturing firms, involving the application of a questionnaire. This questionnaire was tested on 50 manufacturing companies in India. The study reveals that focus on stakeholders; top management commitment and training are critical success factors for implementation of IMS. The main benefits of integration are discussed. The small sample size is one of the major limitations of this study. The paper informs the managers in manufacturing organizations and practitioners of management system standards especially in developing countries about IMS and will enable them to adopt IMS in future so that those organizations may not implement multiple and overlapping MSS(Management System Standards.

  4. CYP1B1 and myocilin gene mutations in Egyptian patients with ...

    African Journals Online (AJOL)

    Purpose: Primary congenital glaucoma (PCG) accounts for 26–29% of childhood blindness in Egypt. The identification of disease causing mutations has not been extensively investigated. We aimed to examine the frequency of CYP1B1 and MYOC mutations in PCG Egyptian patients, and study a possible ...

  5. ATP8B1 deficiency; Pathophysiology and treatment of a cholestatic syndrome with extrahepatic symptoms

    NARCIS (Netherlands)

    Stapelbroek, J.M.

    2009-01-01

    ATP8B1 deficiency is a severe and clinically highly variable hereditary disorder that is primarily characterized by intrahepatic cholestasis. It generally presents as a permanent disorder, progressive familial intrahepatic cholestasis type 1 (PFIC1), or with intermittent cholestasis (benign

  6. 17 CFR 270.30b1-4 - Report of proxy voting record.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Report of proxy voting record... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.30b1-4 Report of proxy voting record....129 of this chapter) not later than August 31 of each year, containing the registrant's proxy voting...

  7. 26 CFR 1.691(b)-1 - Allowance of deductions and credit in respect to decedents.

    Science.gov (United States)

    2010-04-01

    ... TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Income in Respect of Decedents § 1.691(b)-1..., and taxes described in sections 162, 163, 164, and 212 for which the decedent (or a prior decedent... to pay such obligation, as a deduction by the person who by bequest, devise, or inheritance from the...

  8. Gene expression in B-1 cells from lupus-prone mice.

    Science.gov (United States)

    Novaes E Brito, Ronni Rômulo; Xander, Patricia; Pérez, Elizabeth C; Maricato, Juliana T; Laurindo, Maria Fl; De Lorenzo, Beatriz H P; Pellegrino, Renata; Bernardo, Viviane; Lopes, José Daniel; Mariano, Mario

    2014-01-01

    New Zealand Black X New Zealand White F1 [(NZB/NZW)F1] mice develop an autoimmune condition with similarities to human systemic lupus erythematosus (SLE). In this study, we demonstrate that B-1 cells, which have previously been reported to be involved in several autoimmune diseases, have altered gene expression in these mice. RNA was extracted from purified B-1 cells of disease-free C57BL/6 mice and lupus-prone (NZB/NZW)F1 mice. Gene expression was analysed using DNA microarray techniques and validated by real time reverse transcriptase polymerase chain reaction (RT-PCR). In (NZB/NZW)F1 mice, some genes had altered expression patterns compared to disease-free controls. Specifically, the upregulation of Ifitm1, Pvrl2 and Ifi202b and downregulation of Trp53bp1 mRNA were observed in (NZB/NZW)F1 mice. These genes are known to be associated with autoimmune diseases. This pattern of gene expression in B-1 cells could understanding of the pathogenesis of SLE. Thus, it is reasonable to hypothesise that the altered gene expression observed in B-1 cells in our experimental model is important for SLE prognosis and therapy, and these implications are discussed herein.

  9. 17 CFR 270.34b-1 - Sales literature deemed to be misleading.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Sales literature deemed to be... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.34b-1 Sales literature deemed to be misleading. Any advertisement, pamphlet, circular, form letter, or other sales literature addressed to or...

  10. Comparative docking studies of CYP1b1 and its PCG-associated ...

    Indian Academy of Sciences (India)

    PRAKASH KUMAR

    Keywords. CYP1b1; molecular docking; molecular dynamics; oestradiol. Abbreviations used: AA, amino acid; CYP, cytochrome p450; E2, estradiol or oestradiol ; FIR, functionally important region; GA, genetic algorithm; HB, hydrogen bond; HBR, haem-binding region; MD, molecular dynamics; MT, mutant; Nb, non-bonded; ...

  11. Data of evolutionary structure change: 1CF5B-1RTCA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1CF5B-1RTCA 1CF5 1RTC B A --------DVNFDLSTATAKTYTKFIEDFRATL-PFSHK...A 211 1RTC A 1RTCA...ain> 1RTC A 1RTCA SALYYYSTGGTQLPT...pdbChain> 1RTCA MRTRIRYNRRSA HHHHHH 1RTCA AYFFH-PDNQE

  12. B1 but not B2 bradykinin receptor agonists promote DU145 prostate ...

    African Journals Online (AJOL)

    Stimulation of G protein-coupled bradykinin receptors (BR), B1R and. B2R, mediate the effects of bradykinin (BK), which include promotion of cell growth, proliferation and. Corresponding author: Naidoo S. Discipline of Pharmaceutical Sciences,. School of Health Sciences, Nelson R. Mandela School of Medicine campus,.

  13. The vitamin B1 metabolism of Staphylococcus aureus is controlled at enzymatic and transcriptional levels

    NARCIS (Netherlands)

    Müller, Ingrid B; Bergmann, Bärbel; Groves, Matthew R; Couto, Isabel; Amaral, Leonard; Begley, Tadhg P; Walter, Rolf D; Wrenger, Carsten

    2009-01-01

    Vitamin B1 is in its active form thiamine pyrophosphate (TPP), an essential cofactor for several key enzymes in the carbohydrate metabolism. Mammals must salvage this crucial nutrient from their diet in order to complement the deficiency of de novo synthesis. In the human pathogenic bacterium

  14. Potential of lactic acid fermentation in reducing aflatoxin B1 in ...

    African Journals Online (AJOL)

    Aflatoxins are toxic by-products of fungi, with harmful effects on human and animal health. Although maize is known to be highly susceptible to aflatoxin contamination, and a staple in many African countries, there is still lack of methods to mitigate the effects. The effect of lactic acid fermentation on reduction of aflatoxin B1 in ...

  15. Assessment of aflatoxins B 1 , B 2 , G 1 and G 2 status of home ...

    African Journals Online (AJOL)

    Aflatoxins belong to a group of naturally occurring toxins which contaminate foodstuffs under favourable conditions. Aspergillus flavus and Aspergillus parasiticus produce aflatoxins; the four major aflatoxins which contaminate foodstuffs are B1, B2, G1 and G2. Maize, groundnuts, rice, wheat and other foodstuffs are ...

  16. Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells

    Science.gov (United States)

    Changing the optimal tissue culture medium by adding low levels of environmental stress such as 1 µM of the fungal toxin aflatoxin B1 (AFB1), 1 ng of the castor bean protein toxin ricin in transduced mammalian cells or 1% reconstituted milk enhances transcription and increases production of the foll...

  17. 26 CFR 49.4262(b)-1 - Exclusion of certain travel.

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 16 2010-04-01 2010-04-01 true Exclusion of certain travel. 49.4262(b)-1... Exclusion of certain travel. (a) In general. Under section 4262(b) taxable transportation does not include... computing tax on travel not excluded. (1) Where a payment is made for transportation which includes...

  18. Feasibility of detecting Aflatoxin B1 in single maize kernels using hyperspectral imaging

    Science.gov (United States)

    The feasibility of detecting Aflatoxin B1 (AFB1) in single maize kernel inoculated with Aspergillus flavus conidia in the field, as well as its spatial distribution in the kernels, was assessed using near-infrared hyperspectral imaging (HSI) technique. Firstly, an image mask was applied to a pixel-b...

  19. Near-infrared hyperspectral imaging for detecting Aflatoxin B1 of maize kernels

    Science.gov (United States)

    The feasibility of detecting the Aflatoxin B1 in maize kernels inoculated with Aspergillus flavus conidia in the field was assessed using near-infrared hyperspectral imaging technique. After pixel-level calibration, wavelength dependent offset, the masking method was adopted to reduce the noise and ...

  20. 26 CFR 11.401(b)-1 - Certain retroactive changes in plan.

    Science.gov (United States)

    2010-04-01

    ... provision of— (1) A plan as adopted, (2) A plan amendment, or (3) The Employee Income Security Act of 1974... (CONTINUED) INCOME TAX (CONTINUED) TEMPORARY INCOME TAX REGULATIONS UNDER THE EMPLOYEE RETIREMENT INCOME SECURITY ACT OF 1974 § 11.401(b)-1 Certain retroactive changes in plan. (a) General rule. (1) Under section...

  1. Molecular characterization of cytochrome P450 1B1 and effect of ...

    African Journals Online (AJOL)

    FMV

    2013-11-27

    Nov 27, 2013 ... oxidation of endogenous and exogenous compounds, and could potentially be a useful biomarker in fish for exposure to arylhydrocarbon receptors (AhR) ligands. In this study, a new complementary DNA. (cDNA) of the CYP1B subfamily encoding 1B1 was isolated from Nile tilapia (Oreochromis niloticus).

  2. 26 CFR 1.147(b)-1 - Bond maturity limitation-treatment of working capital.

    Science.gov (United States)

    2010-04-01

    ... and Local Bonds § 1.147(b)-1 Bond maturity limitation-treatment of working capital. Section 147(b) does not apply to proceeds of a private activity bond issue used to finance working capital... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Bond maturity limitation-treatment of working...

  3. 26 CFR 1.475(b)-1 - Scope of exemptions from mark-to-market requirement.

    Science.gov (United States)

    2010-04-01

    ... insurance contract (see sections 72, 817, and 7702). (2) Relationships—(i) General rule. The relationships... the meaning of section 475(b)(1)(B)) if it is not held by the taxpayer primarily for sale to customers... or trust, to which the taxpayer has a relationship specified in paragraph (b)(2) of this section; or...

  4. Complex genetics of glaucoma: defects in CYP1B1, and not MYOC ...

    Indian Academy of Sciences (India)

    1997), OPTN (Rezaie et al. 2002) and WDR36 ... recent review (Vasiliou and Gonzalez 2008). Interestingly, it has been ..... 1997). Vincent et al. (2002) suggested MYOC and CYP1B1 might interact through a common pathway and that glaucoma might be multi-allelic in some cases and pro- posed that MYOC function might ...

  5. The frequency of presence of aflatoxin B1 in foodstuffs of vegetable origin

    Directory of Open Access Journals (Sweden)

    Gojković Vesna S.

    2017-01-01

    Full Text Available Cereals, nuts and spices are foods that are used in the daily human diet. According to FAO the average consumption of foods of vegetable origin in people’s diet is increasing. Due to inadequate conditions during storage of foods of vegetable origin, there is possibility of contamination by mold that produces mycotoxins. Since the intake of these products in organism has been increased, there is a risk of exposure to mycotoxins and their harmful effect on the consumers’ health. The aim of this study was to determine the presence of aflatoxin B1 in products of vegetable origin (cereals, nuts and spices. Aflatoxin B1 was determined by enzyme-imunochemical method (ELISA, using commercial kit. 38 samples were tested. In 25 analyzed samples, the content of aflatoxin B1 was higher than 1 μg/kg (1 μg/kg is limit of detection. Out of the total number of tested samples, in 18 samples the content of aflatoxin B1 was determined higher than the allowed amount for this product group by the current regulations (2 μg/kg for cereals, 2 μg/kg for nuts and 5 μg/kg for spices.

  6. A patient with a rare leukodystrophy related to lamin B1 duplication.

    LENUS (Irish Health Repository)

    Molloy, A

    2012-06-01

    The hereditary leukodystrophies are rare disorders caused by molecular abnormalities leading to destruction of or failure of development of central white matter. For almost 30 years there has been increasing recognition of later onset Autosomal Dominant Leukodystrophy (ADLD). We report the first genetically confirmed case of lamin B1 duplication causing ADLD from Ireland.

  7. Chooz B1 start-up marked by total computer control

    International Nuclear Information System (INIS)

    Anon.

    1995-01-01

    The start-up of Chooz B1, the first of EdF's latest generation 1450 MWe N4 reactors, marks the first use in a nuclear power plant of a fully computerised control room. Working in partnership with EdF, Sema Group designed and supplied the advanced command and control system for this plant. (Author)

  8. B1 but not B2 bradykinin receptor agonists promote DU145 prostate ...

    African Journals Online (AJOL)

    Background: The kallikrein-kinin system (KKS) is an endogenous pathway involved in angiogenesis and tumourigenesis, both vital for cancer growth and progression. Objectives: To investigate the effect of two bradykinin receptor (B1R and B2R) agonists on growth and motility of prostate tumour (DU145) and ...

  9. Preparation of B1-(Cr,Ga)N thin films by pulsed laser deposition

    Science.gov (United States)

    Mizuno, Yusei; Nakayama, Tadachika; Suematsu, Hisayuki; Suzuki, Tsuneo

    2018-02-01

    Cr–Ga–N thin films were prepared on (100)-oriented MgO single-crystal substrates by pulsed laser deposition at various substrate temperatures. An ablation plasma, generated by irradiating a Cr–10 at. % Ga alloy target with a Nd:YAG laser, was applied to the substrates to deposit the Cr–Ga–N thin films. Rutherford backscattering spectroscopy revealed that the ratio of the amount of Ga to the amount of Cr plus Ga (hereafter Cr+Ga) in all Cr–Ga–N thin films was approximately 10 at. %. X-ray diffraction and microstructural observation indicated that Cr–Ga–N thin films deposited at substrate temperatures (T sub) above 773 K contained two crystal phases: B1 (NaCl type)-CrN and B4 (würtzite type)-GaN. By contrast, Cr–Ga–N thin films deposited at a T sub of 673 K grew epitaxially and were composed of a single B1-(Cr,Ga)N phase, synthesized by substituting some of the Cr atoms in B1-CrN with Ga atoms. The epitaxial B1-(Cr,Ga)N thin films showed a higher hardness than epitaxial CrN films.

  10. CYP1B1 and myocilin gene mutations in Egyptian patients with ...

    African Journals Online (AJOL)

    Mahmoud R. Fassad

    2016-08-09

    Aug 9, 2016 ... CYP1B1 and myocilin gene mutations in Egyptian patients with primary congenital glaucoma. Mahmoud R. Fassad a. , Asmaa K. Amin a. , Heba A. Morsy a. , Noha M. Issa a. ,. Nader H. Bayoumi b. , Sahar A. El Shafei a. , Soha F. Kholeif a,. * a Department of Human Genetics, Medical Research Institute, ...

  11. Complex genetics of glaucoma: defects in CYP1B1, and not MYOC ...

    Indian Academy of Sciences (India)

    with matched age and ethnicity. Analysis of the family mem- bers reveal that proband's younger sister, ... dence that PCG could be caused by MYOC mutations (Kaur et al. 2005). All these studies point to the fact that although ... primary congenital glaucoma (PCG); CYP1B1; MYOC; human genetics. Journal of Genetics, Vol.

  12. Evaluation of 18F-SFB-Annexin B1 in detecting apoptosis

    International Nuclear Information System (INIS)

    Zhao Qing; Zhang Yingjian; Wang Fang

    2011-01-01

    Objective: To evaluate 18 F-N- succinimidyl -4-fluorobenzoate (SFB)-Annexin B1 in detecting in vitro and in vivo apoptosis. Methods: Anti-Fas antibody was used to induce apoptosis in Jurkat cells. Apoptosis in Jurkat cells was confirmed by flow cytometer (FCM). Unilateral renal ischemia/reperfusion injury was induced by transient (45 min) ligation of the renal artery in the rabbit. The rabbit was then administrated with 18 F-SFB-Annexin B1 intravenously 24 h later and then imaged by PET/CT at 10, 30, 60, 90, 120 and 240 min postinjection. Apoptosis in kidney was confirmed by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL) assay and HE staining. Results The apoptosis rate induced by anti-Fas antibody was 25.98% (120 min) while that in the control group was only 1.81%. The uptake of 18 F-SFB-Annexin B1 in apoptosis group was greater than that in the control group. PET/CT images at 240 min showed higher uptake in the ligated kidney than the non-ligated kidney. TUNEL assay and HE staining confirmed great amount apoptotic cells in the ligated kidney. Conclusion: 18 F-SFB-Annexin B1 may be potentially useful in detecting apoptosis both in vitro and in vivo. (authors)

  13. CYP264B1 from Sorangium cellulosum So ce56: a fascinating norisoprenoid and sesquiterpene hydroxylase.

    Science.gov (United States)

    Ly, Thuy T B; Khatri, Yogan; Zapp, Josef; Hutter, Michael C; Bernhardt, Rita

    2012-07-01

    Many terpenes and terpenoid compounds are known as bioactive substances with desirable fragrance and medicinal activities. Modification of such compounds to yield new derivatives with desired properties is particularly attractive. Cytochrome P450 monooxygenases are potential enzymes for these reactions due to their capability of performing different reactions on a variety of substrates. We report here the characterization of CYP264B1 from Sorangium cellulosum So ce56 as a novel sesquiterpene hydroxylase. CYP264B1 was able to convert various sesquiterpenes including nootkatone and norisoprenoids (α-ionone and β-ionone). Nootkatone, an important grapefruit aromatic sesquiterpenoid, was hydroxylated mainly at position C-13. The product has been shown to have the highest antiproliferative activity compared with other nootkatone derivatives. In addition, CYP264B1 was found to hydroxylate α- and β-ionone, important aroma compounds of floral scents, regioselectively at position C-3. The products, 3-hydroxy-β-ionone and 13-hydroxy-nootkatone, were confirmed by (1)H and (13)C NMR. The kinetics of the product formation was analyzed by high-performance liquid chromatography, and the K ( m ) and k (cat) values were calculated. The results of docking α-/β-ionone and nootkatone into a homology model of CYP264B1 revealed insights into the structural basis of these selective hydroxylations.

  14. Genetic variation in the HSD17B1 gene and risk of prostate cancer.

    Directory of Open Access Journals (Sweden)

    Peter Kraft

    2005-11-01

    Full Text Available Steroid hormones are believed to play an important role in prostate carcinogenesis, but epidemiological evidence linking prostate cancer and steroid hormone genes has been inconclusive, in part due to small sample sizes or incomplete characterization of genetic variation at the locus of interest. Here we report on the results of a comprehensive study of the association between HSD17B1 and prostate cancer by the Breast and Prostate Cancer Cohort Consortium, a large collaborative study. HSD17B1 encodes 17beta-hydroxysteroid dehydrogenase 1, an enzyme that converts dihydroepiandrosterone to the testosterone precursor Delta5-androsterone-3beta,17beta-diol and converts estrone to estradiol. The Breast and Prostate Cancer Cohort Consortium researchers systematically characterized variation in HSD17B1 by targeted resequencing and dense genotyping; selected haplotype-tagging single nucleotide polymorphisms (htSNPs that efficiently predict common variants in U.S. and European whites, Latinos, Japanese Americans, and Native Hawaiians; and genotyped these htSNPs in 8,290 prostate cancer cases and 9,367 study-, age-, and ethnicity-matched controls. We found no evidence that HSD17B1 htSNPs (including the nonsynonymous coding SNP S312G or htSNP haplotypes were associated with risk of prostate cancer or tumor stage in the pooled multiethnic sample or in U.S. and European whites. Analyses stratified by age, body mass index, and family history of disease found no subgroup-specific associations between these HSD17B1 htSNPs and prostate cancer. We found significant evidence of heterogeneity in associations between HSD17B1 haplotypes and prostate cancer across ethnicity: one haplotype had a significant (p < 0.002 inverse association with risk of prostate cancer in Latinos and Japanese Americans but showed no evidence of association in African Americans, Native Hawaiians, or whites. However, the smaller numbers of Latinos and Japanese Americans in this study makes

  15. miR-379 regulates cyclin B1 expression and is decreased in breast cancer.

    Directory of Open Access Journals (Sweden)

    Sonja Khan

    Full Text Available MicroRNAs are small non-coding RNA molecules that control gene expression post-transcriptionally, and are known to be altered in many diseases including breast cancer. The aim of this study was to determine the relevance of miR-379 in breast cancer. miR-379 expression was quantified in clinical samples including tissues from breast cancer patients (n=103, healthy controls (n=30 and patients with benign breast disease (n=35. The level of miR-379 and its putative target Cyclin B1 were investigated on all breast tissue specimens by RQ-PCR. Potential relationships with gene expression and patient clinicopathological details were also determined. The effect of miR-379 on Cyclin B1 protein expression and function was investigated using western blot, immunohistochemistry and proliferation assays respectively. Finally, the levels of circulating miR-379 were determined in whole blood from patients with breast cancer (n=40 and healthy controls (n=34. The level of miR-379 expression was significantly decreased in breast cancer (Mean(SEM 1.9 (0.09 Log10 Relative Quantity (RQ compared to normal breast tissues (2.6 (0.16 Log10 RQ, p<0.01. miR-379 was also found to decrease significantly with increasing tumour stage. A significant negative correlation was determined between miR-379 and Cyclin B1 (r=-0.31, p<0.001. Functional assays revealed reduced proliferation (p<0.05 and decreased Cyclin B1 protein levels following transfection of breast cancer cells with miR-379. Circulating miR-379 was not significantly dysregulated in patients with breast cancer compared to healthy controls (p=0.42. This data presents miR-379 as a novel regulator of Cyclin B1 expression, with significant loss of the miRNA observed in breast tumours.

  16. Effect of vitamin B1 and mixtures of B1 with other vitamins on cytostatica efficiency under irradiation. A study in vitro

    International Nuclear Information System (INIS)

    Heinrich, E.; Getoff, N.

    2002-01-01

    Complete text of publication follows. In the last decades the research of malignant tumors (oncology) became a complex interdisciplinary branch of the modern sciences. In addition to other disciplines the radiation chemistry and radiation biology, applied in this respect, led to new and deeper understanding and knowledge in the radiation therapy of cancer. Following this line and by a combined use of both methods, studies in vitro for testing cytostatica activity and pulse radiolysis for characterisation of the corresponding transients, it was possible to gain a deeper and extensive understanding of complex reaction mechanisms. In addition to valuable biological data we have established that the cytostatica efficiency, e.g. of mitomycin C (MMC) can be very strongly increased in the presence of the vitamins C, E and β-carotene. As an essential step in this respect a cascade electron transfer with the sequence: vit. C → vit. E· + /vit. E → β-car. · + /β-car. → MMC/MMC· - was established. In the present work the ability of vitamin B1 (thiamine) to act under given conditions as cytostaticum or/and as radiation protecting agent will be reported. Extensive investigations in vitro (E. coli bacteria AB 1157 were used as a model) in different media showed that with a combination of vitamin B1 with sanazole (AK-2123) a synergistic effect occurs, which results in a very strong cytostatica enhancement. The presence of vitamin C in the system strongly contributes in this respect. The influence on the sanazole efficiency by other vitamins (E and β-carotene) added individually or in mixture will also be reported. The obtained results are of importance for the radiation therapy of cancer patients. The paper also demonstrates the significant role of radiation chemistry in radiation biology and medicine

  17. Environmental trace analysis by means of supersensitive GC-IMS

    Energy Technology Data Exchange (ETDEWEB)

    Leonhardt, J.W. [IUTLimited, Berlin, (Germany)

    1997-10-01

    The effective control of pollutants in ambient air requires their fast in situ identification and concentration determination of chemical compounds in the range of micrograms per m{sup 3}. There are attempts to use conventional analytical techniques as portable GC and GC-MS. These systems are relatively expensive. A new supersensitive ion Mobility Sensor (IMS) was developed and checked by IUT Ltd, which meets the new demands. The use of tritium sources is an advantage in comparison with other IMS being equipped by nickel-63, the application of which is rather critical in respect of the radiation protection. On the other hand an integrated separation column allows to reduce interferences by matrix effects. The technical parameters of the IUT GC- IMS and some of its most important applications are briefly presented 6 refs., 1 tab., 5 figs.

  18. Information management system study results. Volume 1: IMS study results

    Science.gov (United States)

    1971-01-01

    The information management system (IMS) special emphasis task was performed as an adjunct to the modular space station study, with the objective of providing extended depth of analysis and design in selected key areas of the information management system. Specific objectives included: (1) in-depth studies of IMS requirements and design approaches; (2) design and fabricate breadboard hardware for demonstration and verification of design concepts; (3) provide a technological base to identify potential design problems and influence long range planning (4) develop hardware and techniques to permit long duration, low cost, manned space operations; (5) support SR&T areas where techniques or equipment are considered inadequate; and (6) permit an overall understanding of the IMS as an integrated component of the space station.

  19. Environmental trace analysis by means of supersensitive GC-IMS

    International Nuclear Information System (INIS)

    Leonhardt, J.W.

    1997-01-01

    The effective control of pollutants in ambient air requires their fast in situ identification and concentration determination of chemical compounds in the range of micrograms per m 3 . There are attempts to use conventional analytical techniques as portable GC and GC-MS. These systems are relatively expensive. A new supersensitive ion Mobility Sensor (IMS) was developed and checked by IUT Ltd, which meets the new demands. The use of tritium sources is an advantage in comparison with other IMS being equipped by nickel-63, the application of which is rather critical in respect of the radiation protection. On the other hand an integrated separation column allows to reduce interferences by matrix effects. The technical parameters of the IUT GC- IMS and some of its most important applications are briefly presented

  20. Assessing the Peak Capacity of IMS-IMS Separations of Tryptic Peptide Ions in 300 K He

    Science.gov (United States)

    Merenbloom, Samuel I.; Bohrer, Brian C.; Koeniger, Stormy L.; Clemmer, David E.

    2011-01-01

    Two-dimensional ion mobility spectrometry (IMS-IMS) coupled with mass spectrometry is examined as a means of separating mixtures of tryptic peptides (from myoglobin and hemoglobin). In this study we utilize two distinct drift regions that are identical in that each contains He buffer gas at 300 K. The two-dimensional advantage is realized by changing the structures of the ions. As ions arrive at the end of the first drift region, those of a specified mobility are selected, exposed to energizing collisions, and then introduced into a second drift region. Upon collisional activation some ions undergo structural transitions, leading to substantial changes in their mobilities; others undergo only slight (or no) mobility changes. Examination of peak positions and shapes for peptides that are separated in the first IMS dimension indicates experimental peak capacities ranging from ~60 to 80; the peak shapes and range of changes in mobility that are observed in the second drift region (after activation) indicates a capacity enhancement ranging from a factor of ~7 to 17. Thus, experimental (and theoretical) evaluation of the peak capacity of IMS-IMS operated in this fashion indicates that capacities of ~480 to 1360 are accessible for peptides. Molecular modeling techniques are used to simulate the range of structural changes that would be expected for tryptic peptide ions and are consistent with the experimental shifts that are observed. PMID:17222015

  1. Preoperative imatinib mesylate (IM) for huge gastrointestinal stromal tumors (GIST).

    Science.gov (United States)

    Tang, Sumin; Yin, Yuan; Shen, Chaoyong; Chen, Jiaju; Yin, Xiaonan; Zhang, Bo; Yao, Yuqin; Yang, Jinliang; Chen, Zhixin

    2017-04-11

    Preoperative imatinib mesylate (IM) treatment has not yet been standardized. Here, we aim to further explore such therapy on patients with gastrointestinal stromal tumors (GIST) retrospectively. Patients experiencing preoperative IM were identified from January 2009 to February 2015. A total of 28 GIST patients were identified. The patients received preoperative IM treatment for a median length of 13.5 months, ranging from 5 to 37 months. PR and SD were observed in 24 (85.7%) and 4 (15.3%) patients, respectively. The tumor shrinkage occurred predominantly within 6 to 12 months, and slight tumor shrinkage could be observed after 12 months in certain patients. Nineteen patients (67.9%) received surgery, and R0 resection was acquired in 18 (94.7%) patients. The initial mean maximum diameter was 10.5 (5.2 to 19.0) cm and decreased to 5.9 (2.7 to 19.0) cm after preoperative treatment with a median length of 12 (ranging from 5 to 36) months (P < 0.001) in patients receiving operations. Three in 7 cases of rectum GIST underwent abdominoperineal resection, and four others adopted sphincter-sparing resection. Partial gastrectomy was performed in four patients. IM prior to surgery can effectively prevent tumor rupture and facilitate surgery with low surgical morbidity for GIST patients. Tumor shrinkage following IM occurred predominantly within 6 to 12 months, and slight tumor shrinkage could be observed after 12 months in certain patients. In selected patients, prolonged exposure to IM is seemingly advisable under close radiological surveillance.

  2. Frauen im Netz weltweit – ausgegrenzt und mittendrin

    Directory of Open Access Journals (Sweden)

    Sigrid Peuker

    2003-03-01

    Full Text Available Mit dem Global Center for Women’s Studies and Politics (GLOW ist das Feministische Institut der Heinrich-Böll-Stiftung 1998 online gegangen. Mit diesem Schritt wurden Fragen nach den Möglichkeiten und Grenzen sowie nach den Gestaltungsräumen virtueller Kommunikation für Frauen zentral. Mit feminist_spaces. Frauen im Netz. Diskurse – Communities – Visionen, legt die Heinrich-Böll-Stiftung zusammen mit dem Feministischen Institut Antworten aus internationaler Perspektive vor. Das Buch geht auf die gleichnamige zweitätige Konferenz in Berlin im November 2001 zurück.

  3. IMS IN SMES - REASONS, ADVANTAGES AND BARRIERS ON IMPLEMENTATION

    Directory of Open Access Journals (Sweden)

    Dragan Rajković

    2008-09-01

    Full Text Available Appearance of a number of management systems with various and sometimes divergent demands, demands for revise of optimal strategy on implementation of these standards in small and medium-sized enterprises (SMEs and the attempt on their integration into integrated management system are suggested even more. Firstly question on choice and reasons for implementation of standards is raised. Management and employees expect benefits on the implementation and they pass and minimize the implementation barriers. Basic concept on integrated management system (IMS into SMEs and analyse on reasons, advantages and barriers at IMS implementation are presented in this paper.

  4. Fotografiranje z daljšim časom osvetlitve

    OpenAIRE

    FELTRIN, NUŠA

    2015-01-01

    V diplomskem delu je predstavljena fotografska tehnika, ki se imenuje fotografiranje z daljšim časom osvetlitve. V prvem delu diplome je opisano širše področje fotografije, ki zajema njeno zgodovino, glavne značilnosti in metode fotografiranja. V nadaljevanju je opisana tehnika fotografiranja z daljšim časom osvetlitve, ki predstavlja bistvo diplomske naloge. Opisani so motivi za uporabo tehnike in postopki njene izvedbe. Podane so predstavitve potrebnih pripomočkov, metod in postopkov, ključ...

  5. BENEFITS AT IMS-QES MODEL IN SMEs

    Directory of Open Access Journals (Sweden)

    Dragan Rajković

    2009-03-01

    Full Text Available Appearance of a number of management systems with various and sometimes divergent demands, demands for revise of optimal strategy on implementation of these standards in small and medium-sized enterprises (SMEs and the attempt on their integration into integrated management system are suggested even more. Firstly question on choice and reasons for implementation of standards is raised. Management and employees expect benefits on the implementation and they pass and minimize the implementation barriers. Basic concept on integrated management system (IMS into SMEs and analyse on reasons and advantages at IMS implementation are presented in this paper.

  6. Hinderniserkennung und -verfolgung mit einer PMD-kamera im automobil

    Science.gov (United States)

    Schamm, Thomas; Vacek, Stefan; Natroshvilli, Koba; Marius Zöllner, J.; Dillmann, Rüdiger

    Die Detektion von Hindernissen vor dem Automobil ist eine Hauptanforderung an moderne Fahrerassistenzsysteme (FAS). In dieser Arbeit wird ein System vorgestellt, das mit Hilfe einer PMDKamera (Photomischdetektor) Hindernisse auf der Fahrspur erkennt und deren relevante Parameter bestimmt. Durch die PMD-Kamera werden zunächst 3D-Tiefenbilder der Fahrzeugumwelt generiert. Nach einem initialen Filterprozess werden im Tiefenbild mit Hilfe eines Bereichswachstumsverfahrens Hindernisse gesucht. Zur Stabilisierung des Verfahrens und zur Parameterberechnung wird ein Kaiman Filter eingesetzt. Das Ergebnis ist eine Liste aller Hindernisse im Fahrbereich des Automobils.

  7. Extrahepatic disposition of 3H-aflatoxin B1 in the rainbow trout (Oncorhynchus mykiss)

    International Nuclear Information System (INIS)

    Larsson, P.; Tjaelve, H.; Ngethe, S.; Ingebrigtsen, K.

    1992-01-01

    Whole-body autoradiography in rainbow trout (Oncorhynchus mykiss) after oral and intravenous administration of 3 H-labelled aflation B 1 showed labelling of several extrahepatic tissues, such as the uveal melanin and the vitreous humour of the eyes, the trunk and head kidney, the olfactory rosettes and the pyloric caecae. Liquid chromatography of extracts of the vitreous humour showed that unmetabolized 3 H-AFB 1 was the main labelled material present at this site. Liquid chromatography of extracts of the uveal melanin showed presence of aflatoxicol and aflatoxin B 1 in proportions of about 3:1. The binding to the pigment is probably due to a hydrophobic type of interaction with the melanin. Microautoradiography showed that melanin-containing cells in the trunk and head kidney and in the olfactory rosettes also accumulated high amounts of radioactivity. In the trunk kidney there was, in addition, a labelling of the second segment of the proximal tubules and of the distal tubules and the collecting ducts. Studies in vitro with microsomal and 12,000xg supernatant preparations of the trunk kidney showed formation of DNA- and protein-bound metabolites from the aflatoxin B 1 . It is probable that the bioactivation of the aflatoxin B 1 is confined to the second segment of the proximal tubules. The labelling of the distal tubules and the collecting ducts, which was confined to the cytoplasm of the cells, may be related to excretion and/or absorption processes. Microautoradiography of the olfactory rosettes, showed labelling of the sensory epithelium, but not the indifferent epithelium. A low formation of protein-bound aflatoxin B 1 -metabolites was found in incubations with microsomal preparations of this tissue. The same observation was made in incubations with microsomal preparations of the head kidney. In the pyloric caeca bound metabolites were observed in vivo at a level comparable to that found in the trunk kidney. Our results suggest that retention and metabolism

  8. Hydroxysteroid sulfotransferase SULT2B1b promotes hepatocellular carcinoma cells proliferation in vitro and in vivo.

    Directory of Open Access Journals (Sweden)

    Xiaoming Yang

    Full Text Available Hydroxysteroid sulfotransferase 2B1b (SULT2B1b is highly selective for the addition of sulfate groups to 3β-hydroxysteroids. Although previous reports have suggested that SULT2B1b is correlated with cell proliferation of hepatocytes, the relationship between SULT2B1b and the malignant phenotype of hepatocarcinoma cells was not clear. In the present study, we found that SULT2B1 was comparatively higher in the human hepatocarcinoma tumorous tissues than their adjacent tissues. Besides, SULT2B1b overexpression promoted the growth of the mouse hepatocarcinoma cell line Hepa1-6, while Lentivirus-mediated SULT2B1b interference inhibited growth as assessed by the CCK-8 assay. Likewise, inhibition of SULT2B1b expression induced cell-cycle arrest and apoptosis in Hepa1-6 cells by upregulating the expression of FAS, downregulating the expression of cyclinB1, BCL2 and MYC in vitro and in vivo at both the transcript and protein levels. Knock-down of SULT2B1b expression significantly suppressed tumor growth in nude mouse xenografts. Moreover, proliferation rates and SULT2B1b expression were highly correlated in the human hepatocarcinoma cell lines Huh-7, Hep3B, SMMC-7721 and BEL-7402 cells. Knock-down of SULT2B1b inhibited cell growth and cyclinB1 levels in human hepatocarcinoma cells and suppressed xenograft growth in vivo. In conclusion, SULT2B1b expression promotes proliferation of hepatocellular carcinoma cells in vitro and in vivo, which may contribute to the progression of HCC.

  9. B-1a transitional cells are phenotypically distinct and are lacking in mice deficient in IκBNS

    Science.gov (United States)

    Pedersen, Gabriel K.; Àdori, Monika; Khoenkhoen, Sharesta; Dosenovic, Pia; Beutler, Bruce; Karlsson Hedestam, Gunilla B.

    2014-01-01

    B-1 cells mediate early protection against infection by responding to T cell-independent (TI) antigens found on the surface of various pathogens. Mice with impaired expression of the atypical IκB protein IκBNS have markedly reduced frequencies of B-1 cells. We used a mouse strain with dysfunctional IκBNS derived from an N-ethyl-N-nitrosourea (ENU) screen, named bumble, to investigate the point in the development of B-1 cells where IκBNS is required. The presence of wild-type (wt) peritoneal cells in mixed wt/bumble chimeras did not rescue the development of bumble B-1 cells, but wt peritoneal cells transferred to bumble mice restored natural IgM levels and response to TI antigens. The bumble and wt mice displayed similar levels of fetal liver B-1 progenitors and splenic neonatal transitional B (TrB) cells, both of which were previously shown to give rise to B-1 cells. Interestingly, we found that a subset of wt neonatal TrB cells expressed common B-1a markers (TrB-1a) and that this cell population was absent in the bumble neonatal spleen. Sorted TrB-1a (CD93+IgM+CD5+) cells exclusively generated B-1a cells when adoptively transferred, whereas sorted CD93+IgM+CD5− cells gave rise to B-2 cells and, to a lesser extent, B-1b and B-1a cells. This study identifies a phenotypically distinct splenic population of TrB-1a cells and establishes that the development of B-1a cells is blocked before this stage in the absence of IκBNS. PMID:25228759

  10. ESTIMATION OF AFLATOXIN B1 IN FEED INGREDIENTS AND COMPOUND POULTRY FEEDS

    Directory of Open Access Journals (Sweden)

    Bashir Mahmood Bhatti, Tanzeela Talat and Rozina Sardar

    2001-02-01

    Full Text Available A total of 3230 samples of feed ingredients of vegetable and animal origin and commercially available compound poultry feed received over a period of 5 years at Feed Testing Laboratory of the Institute were tested for Aflatoxin B1 contents (ppb . In all feed ingredients and compound feed stuffs, minimum level of aflatoxin B1 was 13 ppb and maximum level was found to be 78 ppb. No correlation of aflatoxin levels with month of collection of the year which are subject to variation in temperature and humidity could be detected. Mean values of aflatoxin concentration in feed stuffs such as rice, rice polish, wheat bran, wheat bread, maize, fish meal, blood meal, bone meal, guar meal, corn gluten 30%, corn gluten 60%, sun flower meal, soyabean meal and cotton seed meal were found to be higher than safe level of 20 ppb recommended by FDA.

  11. 291-B-1 stack monitoring and sampling system annual system assessment report

    International Nuclear Information System (INIS)

    Ridge, T.M.

    1994-01-01

    The B Plant 291-B-1 main stack exhausts gaseous effluents to the atmosphere from the 221-B Building canyon and cells, the No. 1 Vessel Ventilation System (VVS1), the 212-B Cask Station cell ventilation system, and, to a limited capacity, the 224-B Building. VVS1 collects offgases from various process tanks in 221-B Building, while the 224-B system maintains a negative pressure in out-of-service, sealed process tanks. B Plant Administration Manual, WHC-CM-7-5, Section 5.30 requires an annual system assessment to evaluate and report the present condition of the sampling and monitoring system associated with Stack 291-B-1 (System Number B977A) at B Plant. The system is functional and performing satisfactorily

  12. [Biofilm Formation by the Nonflagellated flhB1 Mutant of Azospirillum brasilense Sp245].

    Science.gov (United States)

    Shelud'ko, A V; Filip'echeva, Yu A; Shumiliva, E M; Khlebtsov, B N; Burov, A M; Petrova, L P; Katsy, E I

    2015-01-01

    Azospirillum brasilense Sp245 with mixed flagellation are able to form biofilms on various surfaces. A nonflagellated mutant of this strain with inactivated chromosomal copy of the flhB gene (flhB1) was shown to exhibit specific traits at the later stages of biofilm formation on a hydrophilic (glass) surface. Mature biofilms of the flhB1::Omegon-Km mutant Sp245.1063 were considerably thinner than those of the parent strain Sp245. The biofilms of the mutant were more susceptible to the forces of hydrodynamic shear. A. brasilense Sp245 cells in biofilms were not found to possess lateral flagella. Cells with polar flagella were, however, revealed by atomic force microscopy of mature native biofilms of strain Sp245. Preservation of a polar flagellum (probably nonmotile) on the cells of A. brasilense Sp245 may enhance the biofilm stability.

  13. Vitamin B1 and B6 in the malaria parasite: requisite or dispensable?

    Directory of Open Access Journals (Sweden)

    C. Wrenger

    Full Text Available Vitamins are essential compounds mainly involved in acting as enzyme co-factors or in response to oxidative stress. In the last two years it became apparent that apicomplexan parasites are able to generate B vitamers such as vitamin B1 and B6 de novo. The biosynthesis pathways responsible for vitamin generation are considered as drug targets, since both provide a high degree of selectivity due to their absence in the human host. This report updates the current knowledge about vitamin B1 and B6 biosynthesis in malaria and other apicomplexan parasites. Owing to the urgent need for novel antimalarials, the significance of the biosynthesis and salvage of these vitamins is critically discussed in terms of parasite survival and their exploitation for drug development.

  14. Design and verification of the Risø-B1 airfoil family for wind turbines

    DEFF Research Database (Denmark)

    Fuglsang, P.; Bak, C.; Gaunaa, M.

    2004-01-01

    severe roughness caused reductions in maximum lift between 12% and 27%. Results for the Risø-B1-24 airfoil showed a maximum lift coefficient of 1.62. The standard case leading edge roughness caused a drop in maximum lift of 7.4%. Vortex generators and Gurney flaps in combination could increase maximum...... wind tunnel, Denmark, at a Reynolds number of 1.6x10(6). For both airfoils the predicted target characteristics were met. Results for Risø-B1-18 showed a maximum lift coefficient of 1.64. A standard case of zigzag tape leading edge roughness caused a drop in maximum lift of only 3.7%. Cases of more...

  15. Emericella astellata, a new producer of aflatoxin B-1, B-2 and sterigmatocystin

    DEFF Research Database (Denmark)

    Frisvad, Jens Christian; Samson, R.A.; Smedsgaard, Jørn

    2004-01-01

    To report on aflatoxin B-1 and B-2 production from a species of Emericella. Methods and Results: Aflatoxins and sterigmatocystin were determined by high-pressure liquid chromatography (HPLC) with diode array detection and confirmed by HPLC with mass spectrometry detection. Among 30 known species...... of Emericella only one species produced aflatoxin. Strains originating from the same geographical source material had different patterns of aflatoxin and sterigmatocystin production on different media, indicating that epigenetic factors may be involved in the regulation of aflatoxin production. However, two...... cultures from the same original genet were very similar. Conclusions: Emericella astellata can produce small amounts of sterigmatocystin and aflatoxin B-1 and B-2. Significance and Impact of the Study: Emericella has been used extensively in genetic studies and therefore the isolates producing aflatoxin...

  16. First-principles study of B1 to B2 phase transition in PbS

    Science.gov (United States)

    Bhambhani, P.; Munjal, N.; Sharma, G.; Vyas, V.; Sharma, B. K.

    2012-07-01

    The high pressure structural phase transition in PbS has been studied by means of first-principles total energy calculations which are based on linear combination of atomic orbitals (LCAO) method within local density approximation (LDA). In the present study, the exchange scheme of Becke and correlation functional of von-Barth-Hedin (VBH) are employed. It is observed that more stable phase for PbS is NaCl type (B1) and PbS transforms to the CsCl type (B2) structure under high pressure (22.8 GPa). The calculated value of transition pressure (Pt) from B1 to B2 structure is found in good agreement with the earlier experimental and theoretical investigations.

  17. The influence of alloying elements in aluminium on the grain refinement with ALTI5B1

    Directory of Open Access Journals (Sweden)

    Naglič I.

    2009-07-01

    Full Text Available This work deals with the influence of alloying elements in aluminium on the grain refinement with various additions of AlTi5B1. Grain-refinement tests were made at a cooling rate of 15 °C/s. The results revealed that in both aluminium and an Al-Fe alloy the grain size decreases with increasing additions of the AlTi5B1 grain refiner. We found that for the same boron content the grain size was smaller in the case of the Al-Fe alloy. The difference in the grain sizes for the same content of boron was approximately 15 μm; this is considerably smaller than the difference between the grain sizes in samples with the same difference of growth-restricting factor made at slower cooling rates.

  18. Alternatives to vitamin B1 uptake revealed with discovery of riboswitches in multiple marine eukaryotic lineages

    OpenAIRE

    McRose, Darcy; Guo, Jian; Monier, Adam; Sudek, Sebastian; Wilken, Susanne; Yan, Shuangchun; Mock, Thomas; Archibald, John M; Begley, Tadhg P; Reyes-Prieto, Adrian; Worden, Alexandra Z

    2014-01-01

    Vitamin B1 (thiamine pyrophosphate, TPP) is essential to all life but scarce in ocean surface waters. In many bacteria and a few eukaryotic groups thiamine biosynthesis genes are controlled by metabolite-sensing mRNA-based gene regulators known as riboswitches. Using available genome sequences and transcriptomes generated from ecologically important marine phytoplankton, we identified 31 new eukaryotic riboswitches. These were found in alveolate, cryptophyte, haptophyte and rhizarian phytopla...

  19. Occurrence of B1 Aflatoxin in diet and M1 Aflatoxin in bovine milk

    OpenAIRE

    Adriana Frizzarin; Thiago Pereira Motta; Thamires Martins; Livia Castelani; Heloisa Solda de Azevedo; Cláudia Rodrigues Pozzi

    2012-01-01

    Ensuring food quality is one of the principles of food safety. Food for dairy cattle may be contaminated by fungi of the genus Aspergillus, which produce aflatoxins. The B1 aflatoxin, when ingested by animals, is biotransformed in liver in several other toxic metabolites, including M1 aflatoxin which is excreted in milk. M1 aflatoxin has a carcinogenic effect, which the presence in milk poses a serious risk to public health because milk and dairy products are consumed mainly by children, preg...

  20. Cleanup Verification Package for the 118-B-1, 105-B Solid Waste Burial Ground

    International Nuclear Information System (INIS)

    Capron, J.M.

    2008-01-01

    This cleanup verification package documents completion of remedial action, sampling activities, and compliance criteria for the 118-B-1, 105-B Solid Waste Burial Ground. This waste site was the primary burial ground for general wastes from the operation of the 105-B Reactor and P-10 Tritium Separation Project and also received waste from the 105-N Reactor. The burial ground received reactor hardware, process piping and tubing, fuel spacers, glassware, electrical components, tritium process wastes, soft wastes and other miscellaneous debris

  1. Exercices de grammaire B1 du cadre européen

    CERN Document Server

    Beaulieu, Christian

    2007-01-01

    Ce cahier d'exercices s'adresse à des apprenants en français. Il présente des activités d'entraînement correspondant au niveau B1 du Cadre Européen commun de référence pour les langues. Ce cahier peut être utilisé en classe ou en autonomie.

  2. Effect of gamma radiation on the inactivation of aflatoxin B1 in food and feed crops

    International Nuclear Information System (INIS)

    Ghanem, I.; Orfi, M.; Shamma, M.

    2007-08-01

    Samples of food crops (peanut, peeled pistachio, unpeeled pistachio, rice, and corn) and feed (barley, bran, corn) were sterilized then inoculated with 10 6 of spore suspension of an isolate of Aspergillus flavus fungus known to produce aflatoxin B1 . Food and feed samples were irradiated with gamma radiation at the doses 4, 6, and 10 kGy. Results indicated that degradation of Aflatoxin B1 was positively correlated with the increase in the applied dose of gamma ray for each tested sample. For example, at a dose of 4 KGy. Percentages of aflatoxin B1 degradation were 8.4, 9.7, 16.6 and 23.5, and 43.97% for peanuts, peeled pistachios, unpeeled pistachios, corn and rice, consecutively . Whereas, at a dose of 10 KGy percentages of aflatoxin degradation reached highest values at 58.6, 68.8, 84.6, 81.1 and 87.8% for peanuts, peeled pistachios, unpeeled pistachios, corn and rice, consecutively In feed samples percentages of aflatoxin degradation were 45, 66, and 90% in barley, 47, 75, and 86% in bran, and 31, 72, and 84% in corn for the doses of 4, 6, and 10 KGy, consecutively. Aflatoxin degradation in food samples correlated negatively with oil content in irradiated samples. Thus, in peanuts, which contained the highest oil content, percentage of aflatoxin degradation at 10 KGy was not more than 56.6%, whereas, the corresponding value in corn, which contained the highest oil content, reached as high as 80%. The above results indicate the possibility of using gamma irradiation as a means of degradation of aflatoxin B1 in food and feed crops to lower than the maximum allowed levels using a maximum dose of radiation of 10 KGy which represents the permitted dose of radiation for such type of crops.(author)

  3. Synthesis and structural characterization of some Pb (B $^{'} _ {1/3 ...

    Indian Academy of Sciences (India)

    Two-stage columbite solid state reaction route has been used for the preparation of Pb (B 1 / 3 ′ Nb2/3)O3 materials (B′ = Mg, Ni and Cd). The columbite precursor phase was structurally characterized using diffraction data. MgNb2O6, NiNb2O6 and CdNb2O6 show orthorhombic structures i.e. pure columbite phase.

  4. Loss of endophilin-B1 exacerbates Alzheimer’s disease pathology

    OpenAIRE

    Wang, David B.; Kinoshita, Yoshito; Kinoshita, Chizuru; Uo, Takuma; Sopher, Bryce L.; Cudaback, Eiron; Keene, C. Dirk; Bilousova, Tina; Gylys, Karen; Case, Amanda; Jayadev, Suman; Wang, Hong-Gang; Garden, Gwenn A.; Morrison, Richard S.

    2015-01-01

    Neuron-specific isoforms of Endophilin-B1, also known as Bax-interacting factor-1 (Bif-1), are neuroprotective. Wang et al. reveal reduced expression of these variants in Alzheimer’s disease, and propose the existence of a feed-forward mechanism whereby beta-amyloid suppresses neuron-specific Bif-1, which in turn enhances beta-amyloid accumulation and neuronal vulnerability to stress.

  5. Genome sequence of the Bacteroides fragilis phage ATCC 51477-B1

    Directory of Open Access Journals (Sweden)

    Hawkins Shawn A

    2008-08-01

    Full Text Available Abstract The genome of a fecal pollution indicator phage, Bacteroides fragilis ATCC 51477-B1, was sequenced and consisted of 44,929 bases with a G+C content of 38.7%. Forty-six putative open reading frames were identified and genes were organized into functional clusters for host specificity, lysis, replication and regulation, and packaging and structural proteins.

  6. Regelungen im Verkehr mit Lebensmitteln und Bedarfsgegenständen in Deutschland

    Science.gov (United States)

    Thomas, Gundula; Freund, Astrid; Gründig, Friedrich

    Im Zuge der Globalisierung von Produktion und Handel ändert sich auch der Charakter der Vorschriften im Lebensmittelrecht. Zunehmend treten internationale Rechtsbestimmungen, Abkommen, Standards und andere Normen an die Stelle nationaler Regelungen.

  7. Rezension: Die Kultur des 20. Jahrhunderts im Überblick - von Werner Faulstich (Hg.

    Directory of Open Access Journals (Sweden)

    Raffaela Rogy

    2013-03-01

    Full Text Available Im Sammelband „Die Kultur des 20. Jahrhunderts im Überblick“ geben AutorInnen wie Knut Hickethier einen kultur-, kunst-, sowie mediengeschichtlichen Abriss des vorherigen Jahrhunderts. Die Rezension hat Raffaela Rogy übernommen.

  8. Im Wind / Mari Vallisoo ; tõlk. Gisbert Jänicke

    Index Scriptorium Estoniae

    Vallisoo, Mari, 1950-2013

    2003-01-01

    Sisu: Im Wind = Tuulega ; Zwei Frauen = Kaks naist ; In Gedanken an Aleksander Levin = aleksander Levinile mõteldes ; Jetzt im Dunkel = Nüüd pimedas ; Fragen = Küsimused ; Der Zwillingsbruder = Kaksikvend

  9. Variant alleles of the CYP1B1 gene are associated with colorectal cancer susceptibility

    International Nuclear Information System (INIS)

    Trubicka, Joanna; Byrski, Tomasz; Gronwald, Jacek; Złowocka, Elżbieta; Kładny, Józef; Banaszkiewicz, Zbigniew; Wiśniowski, Rafał; Kowalska, Elżbieta; Lubinski, Jan; Scott, Rodney J; Grabowska-Kłujszo, Ewa; Suchy, Janina; Masojć, Bartłomiej; Serrano-Fernandez, Pablo; Kurzawski, Grzegorz; Cybulski, Cezary; Górski, Bohdan; Huzarski, Tomasz

    2010-01-01

    CYP1B1 is a P450 enzyme which is involved in the activation of pro-carcinogens to carcinogens as well as sex hormone metabolism. Because differences in the activity of the enzyme have been correlated with variant alleles of single nucleotide polymorphisms (SNPs), it represents an attractive candidate gene for studies into colorectal cancer susceptibility. We genotyped 597 cancer patients and 597controls for three CYP1B1 SNPs, which have previously been shown to be associated with altered enzymatic activity. Using the three SNPs, eight different haplotypes were constructed. The haplotype frequencies were estimated in cases and controls and then compared. The odds ratio for each tumour type, associated with each haplotype was estimated, with reference to the most common haplotype observed in the controls. The three SNPs rs10012, rs1056827 and rs1056836 alone did not provide any significant evidence of association with colorectal cancer risk. Haplotypes of rs1056827 and rs10012 or rs1056827 and rs1056836 revealed an association with colorectal cancer which was significantly stronger in the homozygous carriers. One haplotype was under represented in the colorectal cancer patient group compared to the control population suggesting a protective effect. Genetic variants within the CYP1B1 that are associated with altered function appear to influence susceptibility to a colorectal cancer in Poland. Three haplotypes were associated with altered cancer risk; one conferred protection and two were associated with an increased risk of disease. These observations should be confirmed in other populations

  10. Final characterization report for the 104-B-1 Tritium Vault and 104-B-2 Tritium Laboratory

    International Nuclear Information System (INIS)

    Encke, D.B.; Harris, R.A.

    1996-11-01

    This report is a compilation of the characterization data collected from the 104-B-1 Tritium Vault and the 104-B-2 Trillium Laboratory. The characterization activities were organized and implemented to evaluate the radiological status and identify any hazardous materials. The data contained in this report reflects the current conditions and status of the 104-B-1 Tritium Vault and 104-B-2 Tritium Laboratory. This information is intended to be utilized in support of future building decontamination and demolition, to allow for proper disposal of the demolition debris as required by the Washington Administrative Code, WAC 173-303, the Hanford Site Solid Waste Acceptance Criteria, WHC-EP-0063, and the Environmental Restoration Disposal Facility Waste Acceptance Criteria, BHI-00139. Based on the historical information and facility inspections, the only hazardous materials sampling and analysis activities necessary were to identify lead paint and asbestos containing materials (ACM) in the 104-B-1 Tritium Vault and the 104-B-2 Tritium Laboratory. Asbestos samples were obtained from the outer boundary of the roof areas to confirm the presence and type of asbestos containing fibers. Lead paint samples were obtained to confirm the presence and quantity of lead paint on the roof trim, doors and vents

  11. Fast fat suppression RF pulse train with insensitivity to B1 inhomogeneity for body imaging.

    Science.gov (United States)

    Abe, Takayuki

    2012-02-01

    In higher-field magnetic resonance imaging scanners, a spectrally selective fat saturation radiofrequency (RF) pulse does not work well because B(1) inhomogeneity increases. An adiabatic 180° pulse is used to improve nonuniform fat suppression, but requires inversion recovery time. Therefore, a new RF pulse that achieves flip angles near 90° and is B(1) insensitive has been developed. The pulse consists of three sinc-shaped RF pulses with different flip angles and with different time intervals between each RF pulse. Using the Bloch equations, we analyzed the optimal combination of flip angles. Experimental results demonstrated that M(z) was maintained at less than 0.05 M(0) for a B(1) inhomogeneity of ±35%. The optimal net flip angles was adjusted to 95° by varying the time interval between RF pulses. The pulse duration was 77 ms, which is less than half of the 170-ms inversion recovery time required for the adiabatic pulse. We demonstrated excellent fat suppression for body imaging. Copyright © 2011 Wiley Periodicals, Inc.

  12. Denoising of B1+ field maps for noise-robust image reconstruction in electrical properties tomography

    International Nuclear Information System (INIS)

    Michel, Eric; Hernandez, Daniel; Cho, Min Hyoung; Lee, Soo Yeol

    2014-01-01

    Purpose: To validate the use of adaptive nonlinear filters in reconstructing conductivity and permittivity images from the noisy B 1 + maps in electrical properties tomography (EPT). Methods: In EPT, electrical property images are computed by taking Laplacian of the B 1 + maps. To mitigate the noise amplification in computing the Laplacian, the authors applied adaptive nonlinear denoising filters to the measured complex B 1 + maps. After the denoising process, they computed the Laplacian by central differences. They performed EPT experiments on phantoms and a human brain at 3 T along with corresponding EPT simulations on finite-difference time-domain models. They evaluated the EPT images comparing them with the ones obtained by previous EPT reconstruction methods. Results: In both the EPT simulations and experiments, the nonlinear filtering greatly improved the EPT image quality when evaluated in terms of the mean and standard deviation of the electrical property values at the regions of interest. The proposed method also improved the overall similarity between the reconstructed conductivity images and the true shapes of the conductivity distribution. Conclusions: The nonlinear denoising enabled us to obtain better-quality EPT images of the phantoms and the human brain at 3 T

  13. Evaluation of Extraction and Degradation Methods to Obtain Chickpeasaponin B1 from Chickpea (Cicer arietinum L.

    Directory of Open Access Journals (Sweden)

    Kun Cheng

    2017-02-01

    Full Text Available The objective of this research is to implement extraction and degradation methods for the obtainment of 3-O-[α-l-rhamnopyranosyl-(1→2-β-d-galactopyranosyl] soyasapogenol B (chickpeasaponin B1 from chickpea. The effects of microwave-assisted extraction (MAE processing parameters—such as ethanol concentration, solvent/solid ratio, extraction temperature, microwave irradiation power, and irradiation time—were evaluated. Using 1g of material with 8 mL of 70% aqueous ethanol and an extraction time of 10 min at 70 °C under irradiation power 400W provided optimal extraction conditions. Compared with the conventional extraction techniques, including heat reflux extraction (HRE, Soxhlet extraction (SE, and ultrasonic extraction (UE, MAE produced higher extraction efficiency under a lower extraction time. DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one saponin can be degraded to structurally stable saponin B by the loss of its DDMP group. The influence of pH and the concentration of potassium hydroxide on transformation efficiency of the target compound was investigated. A solution of 0.25 M potassium hydroxide in 75% aqueous ethanol was suitable for converting the corresponding DDMP saponins of chickpeasaponin B1. The implementation by the combining MAE technique and alkaline hydrolysis method for preparing chickpeasaponin B1 provides a convenient technology for future applications.

  14. Facile synthesis of cyclopentenone B1- and L1-type Phytoprostanes

    Directory of Open Access Journals (Sweden)

    Alexandre eGuy

    2015-07-01

    Full Text Available Phytoprostanes (PhytoPs represent non-enzymatic metabolites of α-linolenic acid (ALA, the essential omega-3 polyunsaturated fatty acid (PUFA derived from plants. PhytoPs are present in the plant kingdom and represent endogenous mediators capable of protecting cells from oxidative stress damages in plants. Recently, it was found that such metabolites are present in cooking oil in high quantities, and also that B1-PhytoPs protect immature neurons from oxidant injury and promote differentiation of oligodendrocyte progenitors through PPAR-γ activation. We report a novel and facile synthesis of natural 2,3-substituted cyclopentenone PhytoPs, 16-B1-PhytoP and 9-L1-PhytoP. Our strategy is based on reductive alkylation at the 2-position of 1,3-cyclopentanedione using a recent protocol developed by Ramachary et al., and on a cross-coupling metathesis to access conjugate dienone system. In conclusion, this strategy permitted access to B1- and L1-PhytoPs in a relative short sequence process, and afford the possibility to easily develop analogs of PhytoPs.

  15. Targeting the SR-B1 Receptor as a Gateway for Cancer Therapy and Imaging.

    Science.gov (United States)

    Mooberry, Linda K; Sabnis, Nirupama A; Panchoo, Marlyn; Nagarajan, Bhavani; Lacko, Andras G

    2016-01-01

    Malignant tumors display remarkable heterogeneity to the extent that even at the same tissue site different types of cells with varying genetic background may be found. In contrast, a relatively consistent marker the scavenger receptor type B1 (SR-B1) has been found to be consistently overexpressed by most tumor cells. Scavenger Receptor Class B Type I (SR-BI) is a high density lipoprotein (HDL) receptor that facilitates the uptake of cholesterol esters from circulating lipoproteins. Additional findings suggest a critical role for SR-BI in cholesterol metabolism, signaling, motility, and proliferation of cancer cells and thus a potential major impact in carcinogenesis and metastasis. Recent findings indicate that the level of SR-BI expression correlate with aggressiveness and poor survival in breast and prostate cancer. Moreover, genomic data show that depending on the type of cancer, high or low SR-BI expression may promote poor survival. This review discusses the importance of SR-BI as a diagnostic as well as prognostic indicator of cancer to help elucidate the contributions of this protein to cancer development, progression, and survival. In addition, the SR-B1 receptor has been shown to serve as a potential gateway for the delivery of therapeutic agents when reconstituted high density lipoprotein nanoparticles are used for their transport to cancer cells and tumors. Opportunities for the development of new technologies, particularly in the areas of cancer therapy and tumor imaging are discussed.

  16. Calcium Homeostasis and Muscle Energy Metabolism Are Modified in HspB1-Null Mice

    Directory of Open Access Journals (Sweden)

    Brigitte Picard

    2016-05-01

    Full Text Available Hsp27—encoded by HspB1—is a member of the small heat shock proteins (sHsp, 12–43 kDa (kilodalton family. This protein is constitutively present in a wide variety of tissues and in many cell lines. The abundance of Hsp27 is highest in skeletal muscle, indicating a crucial role for muscle physiology. The protein identified as a beef tenderness biomarker was found at a crucial hub in a functional network involved in beef tenderness. The aim of this study was to analyze the proteins impacted by the targeted invalidation of HspB1 in the Tibialis anterior muscle of the mouse. Comparative proteomics using two-dimensional gel electrophoresis revealed 22 spots that were differentially abundant between HspB1-null mice and their controls that could be identified by mass spectrometry. Eighteen spots were more abundant in the muscle of the mutant mice, and four were less abundant. The proteins impacted by the absence of Hsp27 belonged mainly to calcium homeostasis (Srl and Calsq1, contraction (TnnT3, energy metabolism (Tpi1, Mdh1, PdhB, Ckm, Pygm, ApoA1 and the Hsp proteins family (HspA9. These data suggest a crucial role for these proteins in meat tenderization. The information gained by this study could also be helpful to predict the side effects of Hsp27 depletion in muscle development and pathologies linked to small Hsps.

  17. Stratigraphic structure of the B1 Tertiary tectonostratigraphic unit in eastern Slovenia

    Directory of Open Access Journals (Sweden)

    Bogomir Jelen

    2002-06-01

    Full Text Available High inconsistency and incoherence in the stratigraphy of the Slovenian upper Paleogene and lower Miocene have remained unsolved in the past 150 years. To solve the problem, we tried to rigorously conduct the authentic Galilei’s scientific method. Steps of logical and empirical verification confirmed the existence of the posited B1 Tertiary tectonostratigraphic unit, and a general chronostratigraphic model of new positional relationships of lithologic units resulted from rather good biochronostratigraphic resolution achieved by nannoplankton and planktonic foraminifera biostratigraphy. The application of principles of newly developed fields in science helped us to avoid errors in transmission of messages (to reduce noise from the source (rock to the concept formation,which had been done previously. This in turn has strongly reduced inconsistency andincoherence (high information entropy = uncertainty. The released amount of information enabled us to answer also questions that reached beyond the original difficulty, e.g.: is the tectonostratigraphic structure of eastern Slovenia a manifestation of plate tectonics processes, and of which ones, are theories of continental escape in the Alps and associated dissection and offset of the formerly uniform Slovenian-Hungarian Paleogene basin tenableor not, are then there in the B1 stratigraphic equivalents of the Hungarian Paleogene basin formations, where are the important Eocene / Oligocene, Paleogene / Neogene, Rupelian / Chattian and Kiscellian / Egerian boundaries in Slovenia, and is there acontinuation of the B1 in Croatia and in the Mid-Hungarian tectonic zone?

  18. Simultaneous use of linear and nonlinear gradients for B1+ inhomogeneity correction.

    Science.gov (United States)

    Ertan, Koray; Atalar, Ergin

    2017-09-01

    The simultaneous use of linear spatial encoding magnetic fields (L-SEMs) and nonlinear spatial encoding magnetic fields (N-SEMs) in B 1 + inhomogeneity problems is formulated and demonstrated with both simulations and experiments. Independent excitation k-space variables for N-SEMs are formulated for the simultaneous use of L-SEMs and N-SEMs by assuming a small tip angle. The formulation shows that, when N-SEMs are considered as an independent excitation k-space variable, numerous different k-space trajectories and frequency weightings differing in dimension, length, and energy can be designed for a given target transverse magnetization distribution. The advantage of simultaneous use of L-SEMs and N-SEMs is demonstrated by B 1 + inhomogeneity correction with spoke excitation. To fully utilize the independent k-space formulations, global optimizations are performed for 1D, 2D RF power limited, and 2D RF power unlimited simulations and experiments. Three different cases are compared: L-SEMs alone, N-SEMs alone, and both used simultaneously. In all cases, the simultaneous use of L-SEMs and N-SEMs leads to a decreased standard deviation in the ROI compared with using only L-SEMs or N-SEMs. The simultaneous use of L-SEMs and N-SEMs results in better B 1 + inhomogeneity correction than using only L-SEMs or N-SEMs due to the increased number of degrees of freedom. Copyright © 2017 John Wiley & Sons, Ltd.

  19. Mild phenotype of Charcot-Marie-Tooth disease type 4B1.

    Science.gov (United States)

    Murakami, Tatsufumi; Kutoku, Yumiko; Nishimura, Hirotake; Hayashi, Makiko; Abe, Akiko; Hayasaka, Kiyoshi; Sunada, Yoshihide

    2013-11-15

    Charcot-Marie-Tooth type 4B1 (CMT4B1) is a rare autosomal recessive demyelinating neuropathy caused by mutation of the myotubularin-related 2 (MTMR2) gene. It is characterized by a severe early-onset motor and sensory neuropathy, and myelin outfoldings on nerve biopsy. We describe a mild phenotype of CMT4B1 in a Japanese patient. She noticed difficulty in walking as an initial symptom at age 13. Her symptoms progressed slowly, and she could still walk at age 34. There was no cranial neuropathy. A nerve conduction study demonstrated demyelinating neuropathy. Sural nerve biopsy revealed a moderate-to-severe loss of myelinated fibers, and many focally folded myelin sheaths. Electron micrographs showed myelin outfoldings and infoldings. DNA tests for CMT showed that she is a homozygote for the MTMR2 p.R628PfsX18 mutation. The mild phenotype in our patient is probably due to the C-terminal position of the frame-shift mutation in MTMR2. © 2013 Elsevier B.V. All rights reserved.

  20. Simulation of Fungal-Mediated Cell Death by Fumonisin B1 and Selection of Fumonisin B1–Resistant (fbr) Arabidopsis Mutants

    Science.gov (United States)

    Stone, Julie M.; Heard, Jacqueline E.; Asai, Tsuneaki; Ausubel, Frederick M.

    2000-01-01

    Fumonisin B1 (FB1), a programmed cell death–eliciting toxin produced by the necrotrophic fungal plant pathogen Fusarium moniliforme, was used to simulate pathogen infection in Arabidopsis. Plants infiltrated with 10 μM FB1 and seedlings transferred to agar media containing 1 μM FB1 develop lesions reminiscent of the hypersensitive response, including generation of reactive oxygen intermediates, deposition of phenolic compounds and callose, accumulation of phytoalexin, and expression of pathogenesis-related (PR) genes. Arabidopsis FB1-resistant (fbr) mutants were selected directly by sowing seeds on agar containing 1 μM FB1, on which wild-type seedlings fail to develop. Two mutants chosen for further analyses, fbr1 and fbr2, had altered PR gene expression in response to FB1. fbr1 and fbr2 do not exhibit differential resistance to the avirulent bacterial pathogen Pseudomonas syringae pv maculicola (ES4326) expressing the avirulence gene avrRpt2 but do display enhanced resistance to a virulent isogenic strain that lacks the avirulence gene. Our results demonstrate the utility of FB1 for high-throughput isolation of Arabidopsis defense-related mutants and suggest that pathogen-elicited programmed cell death of host cells may be an important feature of compatible plant–pathogen interactions. PMID:11041878

  1. Imágenes pastorales en la Biblia

    Directory of Open Access Journals (Sweden)

    César Carhuachín

    2015-06-01

    Full Text Available El presente trabajo aborda el tema de imágenes pastorales en la Biblia. Diversas imágenes de Dios han sido hechas en cada cultura, así como también en la Biblia, ya que ésta fue escrita en el contexto del Antiguo Próximo Oriente y Grecia. Este trabajo identifica las imágenes pastorales en Sumeria, Asiria, Babilonia, Egipto y Grecia, destacando similitudes y diferencias en sus usos entre estas naciones e Israel, y entre el Antiguo y Nuevo Testamento. En este análisis, se distingue el uso del término “pastor” como ocupación de otros usos del término transferidos a la divinidad, a los reyes y gobernantes, así como también se distingue del uso Mesías-Pastor de las funciones pastorales del liderazgo cristiano primitivo. El trabajo invita a imaginar a Dios con otras imágenes más relevantes para la vida y cultura latinoamericana, tales como: Dios-Acompañante, Dios-Multiculturalizador/a, Dios-Pacificador/a y Dios-Madre.

  2. Wiedereingliederung in den Arbeitsmarkt im Rahmen beruflicher Rehabilitation

    OpenAIRE

    Wuppinger, Johanna; Rauch, Angela

    2010-01-01

    Erwerbsarbeit ist einer der wichtigsten Aspekte gesellschaftlicher Teilhabe. Berufliche Rehabilitation dient dazu, diese Teilhabe (wieder-) herzustellen und auf Dauer zu sichern. Über die arbeitsmarktintegrative Wirkung dieser Teilhabeleistungen ist allerdings wenig bekannt. Im Gegensatz zum Bereich der medizinischen Rehabilitation ist das Feld der beruflichen Rehabilitation weniger intensiv beforscht. So liegen bisher kaum Erkenntnisse zu Maßnahmeteilnahmen von Rehabilitanden, deren Bewertun...

  3. I'm Positive: Growing Up with Self-Esteem.

    Science.gov (United States)

    Kansas State Univ., Manhattan. Cooperative Extension Service.

    This document presents "I'm Positive: Growing Up With Self-Esteem," an informal, personal study course designed to strengthen the reader's ability to nurture self-esteem in children from birth through adolescence. Special emphasis is given to four parenting skills: acceptance, encouragement, empowerment, and love. Weekly activities are provided…

  4. distance tracking scheme for seamless handover in ims-based ...

    African Journals Online (AJOL)

    userpc

    specified range of best class service for voice traffic and streaming media over IP networks. Keywords: IMS, Handover delay, Distance Tracking, pre-handover, UMTS. INTRODUCTION. Handover is a key process in mobile networks for mobility management and is defined as transferring of the mobile node's connection.

  5. Static corrections for enhanced signal detection at IMS seismic arrays

    Science.gov (United States)

    Wilkins, Neil; Wookey, James; Selby, Neil

    2016-04-01

    Seismic monitoring forms an important part of the International Monitoring System (IMS) for verifying the Comprehensive nuclear Test Ban Treaty (CTBT). Analysis of seismic data can be used to discriminate between nuclear explosions and the tens of thousands of natural earthquakes of similar magnitude that occur every year. This is known as "forensic seismology", and techniques include measuring the P-to-S wave amplitude ratio, the body-to-surface wave magnitude ratio (mb/Ms), and source depth. Measurement of these seismic discriminants requires very high signal-to-noise ratio (SNR) data, and this has led to the development and deployment of seismic arrays as part of the IMS. Array processing methodologies such as stacking can be used, but optimum SNR improvement needs an accurate estimate of the arrival time of the particular seismic phase. To enhance the imaging capability of IMS arrays, we aim to develop site-specific static corrections to the arrival time as a function of frequency, slowness and backazimuth. Here, we present initial results for the IMS TORD array in Niger. Vespagrams are calculated for various events using the F-statistic to clearly identify seismic phases and measure their arrival times. Observed arrival times are compared with those predicted by 1D and 3D velocity models, and residuals are calculated for a range of backazimuths and slownesses. Finally, we demonstrate the improvement in signal fidelity provided by these corrections.

  6. Sixteenth Meeting of the IMS Study Group "Cantus Planus"

    Czech Academy of Sciences Publication Activity Database

    Vozková, Jana

    2011-01-01

    Roč. 48, 2-3 (2011), s. 310-311 ISSN 0018-7003. [Sixteenth Meeting of the IMS Study Group “Cantus Planus”. Vídeň, 21.08.2011–27.08.2011] Institutional research plan: CEZ:AV0Z90580513 Keywords : plainchant * annual conference * International Musicological Society Subject RIV: AL - Art, Architecture, Cultural Heritage

  7. Using the IMS LD Standard to Describe Learning Designs

    NARCIS (Netherlands)

    Koper, Rob; Miao, Yongwu

    2007-01-01

    Koper, R., & Miao, Y. (2008). Using the IMS LD Standard to Describe Learning Designs. In L. Lockyer, S. Bennet, S. Agostinho & B. Harper (Eds.), Handbook of Research on Learning Design and Learning Objects: Issues, Applications and Technologies (pp. 41-86). IDEA group.

  8. IMS Learning Design Version 1.0 Final Specification

    NARCIS (Netherlands)

    Koper, Rob; Manderveld, Jocelyn; Sloep, Peter; Hummel, Hans; Vogten, Hubert; Brouns, Francis; Tattersall, Colin

    2006-01-01

    The IMS Learning Design specification supports the use of a wide range of pedagogies in online learning. Rather than attempting to capture the specifics of many pedagogies, it does this by providing a generic and flexible language. This language is designed to enable many different pedagogies to be

  9. Address Translation Problems in IMS Based Next Generation Networks

    Directory of Open Access Journals (Sweden)

    Balazs Godor

    2006-01-01

    Full Text Available The development of packed based multimedia networks reached a turning point when the ITU-T and the ETSIhave incorporated the IMS to the NGN. With the fast development of mobile communication more and more services andcontent are available. In contrast with fix network telephony both the services and the devices are personalized in the “mobileworld”. Services, known from the Internet - like e-mail, chat, browsing, presence, etc. – are already available via mobiledevices as well. The IMS originally wanted to exploit both the benefits of mobile networks and the fancy services of theInternet. But today it is already more than that. IMS is the core of the next generation telecommunication networks and abasis for fix-mobile convergent services. The fact however that IMS was originally a “mobile” standard, where IPv6 was notoddity generated some problems for the fix networks, where IPv4 is used. In this article I give an overview of these problemsand mention some solutions as well.

  10. Distance tracking scheme for seamless handover in IMS-based ...

    African Journals Online (AJOL)

    This paper proposes a fast and seamless handover scheme for systems based on IP Multimedia Subsystem (IMS) architectural framework with Universal Mobile Telecommunications System (UMTS) access network. In the scheme the location, direction and movement pattern of a Mobile Node (MN) in a network cell are ...

  11. First inter metatarsal (IM) angles in Nigerians and their relationship ...

    African Journals Online (AJOL)

    Background: No age is immune to the deformity known as Hallux valgus as it has been proven that 50% of adults developed it during adolescence, while 40% of juveniles developed it before the age of 10.5 years. Aim: To determine from radiographs the normal value of the first inter metatarsal (IM) angle and to establish if ...

  12. Mapping IMS Learning Design and Moodle. A first understanding

    NARCIS (Netherlands)

    Burgos, Daniel; Tattersall, Colin; Dougiamas, Martin; Vogten, Hubert; Koper, Rob

    2006-01-01

    Please, cite this publication as follows: Burgos, D., Tattersall, C., Dougiamas, M., Vogten, H., & Koper, E. J. R. (2006). Mapping IMS Learning Design and Moodle. A first understanding. Proceedings of Simposo Internacional de Informática Educativa (SIIE06), León, Spain: IEEE Technical Committee on

  13. Substandardaussprache - (k)ein Thema im DaF-Unterricht

    DEFF Research Database (Denmark)

    Bonner, Maria

    2011-01-01

    Der Umgang mit Norm und Variation ist ein Problem im DaF-Unterricht, insbesondere bei der Aussprache. Auf der einen Seite steht die in den Aussprachewörterbüchern kodifizierte Norm der Standardaussprache, auf der anderen die Sprechwirklichkeit, die in ihrer gesamten Variationsbreite auch Muttersp...

  14. Ausländische Direktinvestitionen und Technologietransfer im angolanischen Energiesektor

    Directory of Open Access Journals (Sweden)

    2016-04-01

    Full Text Available Dem Zusammenhang von ausländischen Direktinvestionen (Foreign Direct Investment, FDI und Technologietransfer wird sowohl in der Forschung als auch in der politischen Analyse große Bedeutung zugeschrieben. Zahlreiche Veröffentlichungen belegen, dass FDI den Transfer von und/oder den Zugang zu Technologien erleichtert. Allerdings gibt es zu wenige empirische Studien, die diesen Zusammenhang in Bezug auf die Wirtschaft afrikanischer Staaten untersuchen. Der Autor will diese Lücke zu einem kleinen Teil schließen, indem er die Bedeutung des Technologietransfers durch den Zufluss von FDI im Energiesektor Angolas ermittelt. Seine Analyse basiert auf qualitativer Forschung in Angola im Jahr 2014. Er zeigt auf, dass sich die Erzeugung von Energie(trägern und die Verteilungsinfrastruktur - maschinelle Ausrüstung und Qualifizierung - durch den Zufluss von FDI erheblich entwickeln konnte. Allerdings gebe es keinen Beleg dafür, dass dieser Zufluss die endogenen wissenschaftlichen und technologischen Forschungspotenziale im angolanischen Energiesektor gefördert hat. Der Autor empfiehlt politische Maßnahmen zur Förderung dieser Fähigkeiten, insbesondere im Bereich der Produktion.

  15. The (Im)possibility of the Project: Radford Address

    Science.gov (United States)

    Green, Bill

    2010-01-01

    In this address, the author engages both with the possibility "and" the impossibility of the educational project--and suggests something of what it means to say this. His presentation is specifically addressed to the theme of the (im)possibility of the educational project. He draws from philosophy, literature, psychoanalysis and history,…

  16. Entwicklung und Evolution dienstorientierter Anwendungen im Web Engineering

    OpenAIRE

    Nußbaumer, Martin

    2008-01-01

    Die vorliegende Abhandlung untersucht die methodische und systematische Herangehensweise an die Entwicklung dienstorientierter Anwendungen im Web Engineering. Dabei wird deren architekturelle Betrachtung in den Vordergrund gerückt, die auf der Grundlage autonomer, wiederverwendbarer Komponenten in Form von Web Services basiert. Dazu werden dedizierte Modelle, Methoden und Werkzeuge entlang dieser - an Web-Standards orientierten - Architektur entwickelt.

  17. Using IMS Learning Design to model collaborative learning activities

    NARCIS (Netherlands)

    Tattersall, Colin

    2006-01-01

    IMS Learning Design provides a counter to the trend towards designing for lone-learners reading from screens. It guides staff and educational developers to start not with content, but with learning activities and the achievement of learning objectives. It recognises that learning can happen without

  18. Status Quo von Fm im europäischen Vergleich - die Trendanalyse im Jahresvergleich ab 2005

    Directory of Open Access Journals (Sweden)

    Redlein, Alexander

    2018-01-01

    Full Text Available Facility Management (FM ist ganzheitliches Management der Immobilien und der immateriellen/materiellen Infrastruktur einer Organisation auf strategischer und taktischer Ebene, um die Erbringung der vereinbarten Unterstützungsleistungen (Facility Services zu koordinieren. Ziel ist die Verbesserung der Produktivität des Kerngeschäfts. Seit dem Jahr 2005 führt das IFM der Technischen Universität Wien (TU Wien jährlich Untersuchungen der Nachfrage- bzw. Bedarfsseite des FM in verschiedenen europäischen Ländern durch. Ziel ist es dabei, einen Marktüberblick zu erhalten und neben der Darstellung der allgemeinen Entwicklung und Bedeutung des FM, mehr zur Wirtschaftlichkeit und Nachhaltigkeit des FM herauszufinden. Wichtige Ergebnisse, beispielweise Kostentreiber, konnten aufgrund der bisher durchgeführten Studien und Analysen bereits abgeleitet werden. Durch die Ausweitung des Datenmaterials und somit des Stichprobenumfangs auf weitere Länder lassen sich nun noch aussagekräftigere Auswertungen und Analysen bewerkstelligen. Ländervergleiche über den Status Quo der FM-Bedarfsseite werden somit ermöglicht. Gemeinsamkeiten, Unterschiede und die Marktreife des FM in den analysierten Ländern werden dargestellt und (statistisch analysiert. Die Erhebung des Status Quo der Bedarfsseite im FM in Europa zeigt die unterschiedlichen Reifegrade deutlich auf.

  19. Occurrence of B1 Aflatoxin in diet and M1 Aflatoxin in bovine milk

    Directory of Open Access Journals (Sweden)

    Adriana Frizzarin

    2012-12-01

    Full Text Available Ensuring food quality is one of the principles of food safety. Food for dairy cattle may be contaminated by fungi of the genus Aspergillus, which produce aflatoxins. The B1 aflatoxin, when ingested by animals, is biotransformed in liver in several other toxic metabolites, including M1 aflatoxin which is excreted in milk. M1 aflatoxin has a carcinogenic effect, which the presence in milk poses a serious risk to public health because milk and dairy products are consumed mainly by children, pregnant women and elderly. The objective of this study was to detect the presence of B1 aflatoxin in feed supplied to dairy cows and the presence of M1 aflatoxin in milk. Samples were collected from complete diet (corn silage and concentrate from a batch of 15 lactating cows from a dairy farm in the Campinas region. Two samples of diets were collected directly into the troughs in intervals of 24 hours at every 15 days, totalizing a period of 45 days. Milk samples of those cows were collected 24 hours after diet collection, directly from sample valves in the glass jars.. B1 and M1 aflatoxins were detected by the technique of High Performance Liquid Chromatography after extraction and purification on immunoaffinity columns. From the 40 samples of diets evaluated, 40% were contaminated with B1 aflatoxin, and the levels found ranged from 1.93 to 43.78μg/Kg. One sample showed result higher than the maximum recommended for grain and animal feed in Brazil (20μg/Kg. From the 75 milk samples analyzed, the presence of M1 aflatoxin was detected in 13.3% with levels ranging from 0.03 to 0.16μg/L, not exceeding the maximum permitted for marketing in the country of 0.5μg/L, however 80% of contaminated samples had values above the maximum permissible levels of 0.05μg/L, value found among countries with abundant milk production... The presence of aflatoxins highlights the importance of monitoring the production, the storage and the importance of handling food and

  20. Flexible Authoring and Delivery of Online Courses Using IMS Learning Design

    Science.gov (United States)

    Hermans, Henry; Janssen, José; Koper, Rob

    2016-01-01

    Since the publication of the IMS Learning Design (IMS LD) specification in 2003, many initiatives have been undertaken to build authoring tools that are simple enough to be used by non-technical instructors and teachers. IMS LD's technical complexity is believed to be a major burden for the adoption of the specification. We have developed a new…

  1. School Burnout im Jugendalter : Die Rollen von Eltern und Lehrpersonen bei School Burnout im Jugendalter und deren Einbezug in die Ergotherapie

    OpenAIRE

    Fehr, Andrea

    2016-01-01

    Hintergrund: Jugendliche erleben Stress und Antriebslosigkeit im Schulkontext. Eine steigende Anzahl Studien widmet sich dem Phänomen School Burnout (SB), welches drei Komponenten umfasst: Erschöpfung, Zynismus gegenüber der Schule und das Gefühl von Unangemessenheit in der Rolle als Schüler. Die Interaktionen im sozialen Kontext beeinflussen die Entwicklung von Motivation und Verhalten im Jugendalter wesentlich. Ziel: Diese Bachelorarbeit hat zum Ziel, die Rolle von Eltern und Le...

  2. Downregulation of NF-ΚB1 enhances the radiosensitivity of renal cell carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Ikegami, Amanda; Silva, Luiz Felipe Teixeira da; Bellini, Maria Helena [Instituto De Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), São Paulo, SP (Brazil)

    2017-07-01

    Full text: Introduction: Clear cell renal cell carcinoma (ccRCC) accounts for ∼80% of all renal cell carcinomas (RCC) and has the Von Hippel-Lindau (VHL) tumor suppressor gene mutated. The lack of VHL protein leads to a constitutionally active Hypoxia Inducible Factor (HIF) pathway that confers both chemoresistance and radioresistance for renal tumor. HIF pathway is known to interact with the transcription factor nuclear factor kappa B (NF-kB). Increased NF-κB activity is associated with the development and progression of RCC (IKEGAMI A, TEIXEIRA LF. BRAGA MS et al. The American Society for Cell Biology 2016; 26: 3948-3955). Objective: Evaluate the synergistic effect of NF-kB1 knockdown and ionizing radiation in murine renal adenocarcinoma cell line. Methods: The murine renal adenocarcinoma cell line (Renca cells) (ATCC, USA) was cultured in RPMI 1640 supplemented with 10% FBS and penicillin/streptomycin. Lentiviral shRNA vector was used to knockdown of NF-KB1 gene in Renca cells, as described previously (1). In the clonogenic cell survival assay, the cells were irradiated by {sup 60}Co source in the range from 0 to 10 Gy, using the GammaCell 220 – Irradiation Unit of Canadian-Atomic Energy Commision Ltd. (CTR-IPEN). After 10-14 days of culture, cell colonies were fixed and stained with formaldehyde 4% and rhodamine B 2% and counted. To assess cell viability, tetrazolium [3-(4,5-dimethylthiazol-2-yl)-5- (3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-MTS] was performed within 24 hours after irradiation at a dose of 10Gy. The survival variables α e β were fitted according to the linear quadratic equation (SF=exp[-αD-βD2]); SF=survival fraction, D=dose of irradiation and P value was determined by F test. Multiple comparisons were assessed by One-way ANOVA followed by Bonferroni´s tests with GraphPad Prism version 6.0 software. P< 0.05 was considered statistically significant. Data are shown as the mean ± SD. Results: The Renca-shRNA-NF-kB1 cells were found

  3. Suicidal gene therapy with rabbit cytochrome P450 4B1/4-ipomeanol, 2-aminoanthracene system in glioma cell

    Energy Technology Data Exchange (ETDEWEB)

    Jang, Su Jin; Kang, Joo Hyun; Kim, Kwang Il; Lee, Tae Sup; Lee, Yong Jin; Woo, Kwang Sun; Chung, Wee Sup; Cheon, Gi Jeong; Choi, Chang Woon; Lim, Sang Moo [Korea Institute of Radiological and Medical Sciences, Seoul (Korea, Republic of)

    2010-10-15

    Suicidal gene therapy is based on the transduction of tumor cells with 'suicide' genes encoding for prodrugactivating enzymes that render target cells susceptible to prodrug treatment. Suicidal gene therapy results in the death of tumor with the expression of gene encoding enzyme that converts non-toxic prodrug into cytotoxic product. Cytochrome P450 4B1 (CYP4B1) activates 4- ipomeanol (4-ipo) and 2-aminoanthracene (2-AA) to cytotoxic furane epoxide and unsaturated dialdehyde intermediate. In this study, therapeutic effects of suicidal gene therapy with rabbit CYP4B1/4-ipo or CYP4B1/2-AA system

  4. Suicidal gene therapy with rabbit cytochrome P450 4B1/4-ipomeanol, 2-aminoanthracene system in glioma cell

    International Nuclear Information System (INIS)

    Jang, Su Jin; Kang, Joo Hyun; Kim, Kwang Il; Lee, Tae Sup; Lee, Yong Jin; Woo, Kwang Sun; Chung, Wee Sup; Cheon, Gi Jeong; Choi, Chang Woon; Lim, Sang Moo

    2010-01-01

    Suicidal gene therapy is based on the transduction of tumor cells with 'suicide' genes encoding for prodrugactivating enzymes that render target cells susceptible to prodrug treatment. Suicidal gene therapy results in the death of tumor with the expression of gene encoding enzyme that converts non-toxic prodrug into cytotoxic product. Cytochrome P450 4B1 (CYP4B1) activates 4- ipomeanol (4-ipo) and 2-aminoanthracene (2-AA) to cytotoxic furane epoxide and unsaturated dialdehyde intermediate. In this study, therapeutic effects of suicidal gene therapy with rabbit CYP4B1/4-ipo or CYP4B1/2-AA system

  5. Decreased internalisation of erbB1 mutants in lung cancer is linked with a mechanism conferring sensitivity to gefitinib.

    Science.gov (United States)

    Hendriks, B S; Griffiths, G J; Benson, R; Kenyon, D; Lazzara, M; Swinton, J; Beck, S; Hickinson, M; Beusmans, J M; Lauffenburger, D; de Graaf, D

    2006-11-01

    A majority of gefitinib (IRESSA)-responsive tumours in non-small cell lung cancer have been found to carry mutations in ErbB1. Previously, it has been observed that internalisation-deficient ErbB1 receptors are strong drivers of oncogenesis. Using a computational model of ErbB1 trafficking and signalling, it is found that a deficiency in ErbB1 internalisation is sufficient to explain the observed signalling phenotype of these gefitinib-responsive ErbB1 mutants in lung cancer cell lines. Experimental tests confirm that gefitinib-sensitive cell lines with and without ErbB1 mutations exhibit markedly slower internalisation rates than gefitinib-insensitive cell lines. Moreover, the computational model demonstrates that reduced ErbB1 internalisation rates are mechanistically linked to upregulated AKT signalling. Experimentally it is confirmed that impaired internalisation of ErbB1 is associated with increased AKT activity, which can be blocked by gefitinib. On the basis of these experimental and computational results, it is surmised that gefitinib sensitivity is a marker of a reliance on AKT signalling for cell survival that may be brought about by impaired ErbB1 internalisation.

  6. Heterogeneous nuclear ribonucleoprotein B1 protein impairs DNA repair mediated through the inhibition of DNA-dependent protein kinase activity

    International Nuclear Information System (INIS)

    Iwanaga, Kentaro; Sueoka, Naoko; Sato, Akemi; Hayashi, Shinichiro; Sueoka, Eisaburo

    2005-01-01

    Heterogeneous nuclear ribonucleoprotein B1, an RNA binding protein, is overexpressed from the early stage of lung cancers; it is evident even in bronchial dysplasia, a premalignant lesion. We evaluated the proteins bound with hnRNP B1 and found that hnRNP B1 interacted with DNA-dependent protein kinase (DNA-PK) complex, and recombinant hnRNP B1 protein dose-dependently inhibited DNA-PK activity in vitro. To test the effect of hnRNP B1 on DNA repair, we performed comet assay after irradiation, using normal human bronchial epithelial (HBE) cells treated with siRNA for hnRNP A2/B1: reduction of hnRNP B1 treated with siRNA for hnRNP A2/B1 induced faster DNA repair in normal HBE cells. Considering these results, we assume that overexpression of hnRNP B1 occurring in the early stage of carcinogenesis inhibits DNA-PK activity, resulting in subsequent accumulation of erroneous rejoining of DNA double-strand breaks, causing tumor progression

  7. RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane

    Czech Academy of Sciences Publication Activity Database

    Sabol, P.; Kulich, I.; Žárský, Viktor

    2017-01-01

    Roč. 68, č. 12 (2017), s. 3253-3265 ISSN 0022-0957 R&D Projects: GA ČR(CZ) GA15-14886S Institutional support: RVO:61389030 Keywords : oligomeric golgi-complex * powdery mildew fungus * pseudomonas-syringae * cell polarity * arabidopsis * protein * plants * resistance * expression * transport * Autophagy * exo70b1 * exo70b2 * exocyst * plant immunity * rin4 * secretion Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 5.830, year: 2016

  8. Periprosthetic Vancouver type B1 and C fractures treated by locking-plate osteosynthesis

    DEFF Research Database (Denmark)

    Froberg, Lonnie; Troelsen, Anders; Brix, Michael

    2012-01-01

    Historically, the treatment of periprosthetic femoral fractures (PFFs) has been associated with a high frequency of complications and reoperations. The preferred treatment is internal fixation, a revision of the femoral stem, or a combination of both. An improved understanding of plate use during...... internal fixation, and the introduction of locking-plate osteosynthesis may lead to improved outcome. We evaluated the outcome of Vancouver type B1 and C PFFs treated by locking-plate osteosynthesis, by assessing rates of fracture union and reoperations and by analyzing failure cases....

  9. Aflatoxin B1and sterigmatocystin survey in beer sold in China.

    Science.gov (United States)

    Zhao, Yarong; Huang, Jianxiang; Ma, Liyan; Liu, Shuai; Wang, Fuhua

    2017-03-01

    A total of 101 samples of beer from the Chinese market were analysed for the presence of aflatoxin B 1 (AFB 1 ) and sterigmatocystin (STC), using methods based on liquid chromatography-tandem mass spectrometry. The limit of quantification and the limit of detection in beer were 0.1 and 0.03 µg/kg, respectively. Recoveries of AFB 1 and STC from spiked beer samples were 97.8-103.6% and 92.7-102.1%, respectively. None of the beer purchased samples were contaminated with AFB 1 or STC.

  10. Degradation of Aflatoxin B1 during the Fermentation of Alcoholic Beverages

    OpenAIRE

    Naoki Mochizuki; Yasushi Nagatomi; Atsuo Uyama; Tomonori Inoue

    2013-01-01

    Aflatoxin B1 (AFB1) is a contaminant of grain and fruit and has one of the highest levels of carcinogenicity of any natural toxin. AFB1 and the fungi that produce it can also contaminate the raw materials used for beer and wine manufacture, such as corn and grapes. Therefore, brewers must ensure strict monitoring to reduce the risk of contamination. In this study, the fate of AFB1 during the fermentation process was investigated using laboratory-scale bottom and top beer fermentation and wine...

  11. Thiamin (Vitamin B1) Biosynthesis and Regulation: A Rich Source of Antimicrobial Drug Targets?

    Science.gov (United States)

    Du, Qinglin; Wang, Honghai; Xie, Jianping

    2011-01-01

    Drug resistance of pathogens has necessitated the identification of novel targets for antibiotics. Thiamin (vitamin B1) is an essential cofactor for all organisms in its active form thiamin diphosphate (ThDP). Therefore, its metabolic pathways might be one largely untapped source of antibiotics targets. This review describes bacterial thiamin biosynthetic, salvage, and transport pathways. Essential thiamin synthetic enzymes such as Dxs and ThiE are proposed as promising drug targets. The regulation mechanism of thiamin biosynthesis by ThDP riboswitch is also discussed. As drug targets of existing antimicrobial compound pyrithiamin, the ThDP riboswitch might serves as alternative targets for more antibiotics. PMID:21234302

  12. Effect of Mycoplasma hyopneumoniae and fumonisin B1 toxin on the lung in pigs

    Directory of Open Access Journals (Sweden)

    Melinda Kovács

    2010-01-01

    Full Text Available The authors examined the combined effect of Mycoplasma hyopneumoniae (Mh and fumonisin B1 (FB1 mycotoxin in pigs. Computed tomography (CT was applied to follow up the pathological events in the lung. Piglets were infected with Mh, or treated with FB1, or both infected and treated with Mh and FB1. The Mh infection produced lung lesions in all piglets the severity of which was increased by FB1. The CT is a suitable method for studying the pathological conditions in the lower respiratory tract of swine.

  13. TU-H-206-03: Characterizing B1 Inhomogeneities in DCE MRI

    International Nuclear Information System (INIS)

    Gach, H; Mason, N

    2016-01-01

    Purpose: Dynamic Contrast Enhanced (DCE) MRI is a valuable technique for measuring perfusion and permeability characteristics of tumors. Exogenous contrast concentrations are calculated based on changes in T 1 measured using fast 3D gradient echo (FLASH) sequences. However, the slab selective pulses used in 3D MRI may result in B 1 inhomogeneities across the volume of interest that can lead to errors in T 1 and thus the estimated gadolinium concentration. We compared three FLASH DCE sequences (GRE, TWIST, and VIBE) to determine their signal homogeneity across slices and the accuracy in calculating T 1 using acquisitions with variable flip angles. Methods: The sequences were tested at 3 T on a Siemens mMR (VB20P) using a doped water phantom 3.75 g/L NiSO 4 - 6H 2 O + 5 g/L NaCl (T 1 = 104 ms) and a 2% agar, 0.67% NaCl phantom (T 1 = 1.71 s). 2D EPI B 1 maps and inversion recovery T 1 maps were acquired for ground truth. 3D MRI was acquired at different flip angles to generate a T 1 map. Regions of interest were drawn to measure signal inside the phantoms as a function of slice position. The T 1 for each slice ROI was fit to the FLASH steady-state model of magnetization. Results: Based on the data, GRE gave the most uniform signal homogeneity and T 1 values in the middle slices of the 3D volume. The 3D VIBE sequence had the largest region of signal inhomogeneity compared to the 3D GRE and TWIST sequences. VIBE’s B 1 inhomogeneity is inconsistent at low flip angles. However, VIBE resulted in more slices with T 1 values similar to the ground truth. Conclusion: The central 1/3 of the slices yielded signals that result in T 1 fits consistent with the ground truth. However, the remaining slices required some form of B 1 inhomogeneity correction for quantitative DCE analysis. The research was supported in part by NIH NCI Grant R01CA159471.

  14. Yeast cell based feed additives: Studies on aflatoxin B1 and zearalenone

    OpenAIRE

    2011-01-01

    Abstract Thirty commercially available yeast cell wall products and two reference bentonites were tested for their ability to bind aflatoxin B1 (AFB1) and zearalenone (ZON) in buffer solutions at pH 3 and pH 6.5 as well as in real gastric juice. For most products, the binding efficacy of AFB1 correlated with the ash content which was between 2.6 and 89% and constituted the inorganic non-volatile components, like mineral clays, of the samples. Samples with smectite as main ash compo...

  15. No evidence of association between mutant alleles of the CYP27B1 gene and MS

    Science.gov (United States)

    Ban, Maria; Caillier, Stacy; Mero, Inger-Lise; Myhr, Kjell-Morten; Celius, Elisabeth G.; Aarseth, Jan; Torkildsen, Øivind; Harbo, Hanne F.; Oksenberg, Jorge; Hauser, Stephen L.; Sawcer, Stephen; Compston, Alastair

    2012-01-01

    An association has previously been reported between susceptibility to multiple sclerosis and the rare mutant alleles of the CYP27B1 gene responsible for autosomal recessive Vitamin D Dependent Rickets type 1 (VDDR1). In an attempt to replicate this finding, we screened 495 multiplex families and 2092 single affected families, together with 4594 cases and 3583 controls (a total of 17073 individuals) but were unable to find any evidence supporting this putative association. Our data do not indicate that mutations responsible for VDDR1 influence the risk of developing multiple sclerosis. PMID:23444327

  16. Genetic variation of Aflatoxin B(1) aldehyde reductase genes (AFAR) in human tumour cells

    DEFF Research Database (Denmark)

    Praml, Christian; Schulz, Wolfgang; Claas, Andreas

    2008-01-01

    AFAR genes play a key role in the detoxification of the carcinogen Aflatoxin B(1) (AFB(1)). In the rat, Afar1 induction can prevent AFB(1)-induced liver cancer. It has been proposed that AFAR enzymes can metabolise endogenous diketones and dialdehydes that may be cytotoxic and/or genotoxic. Furth...... many aldo-keto reductases. This polarity change may have an effect on the proposed substrate binding amino acids nearby (Met(47), Tyr(48), Asp(50)). Further population analyses and functional studies of the nine variants detected may show if these variants are disease-related....

  17. Usability test of the ImPRO, computer-based procedure system

    International Nuclear Information System (INIS)

    Jung, Y.; Lee, J.

    2006-01-01

    ImPRO is a computer based procedure in both flowchart and success logic tree. It is evaluated on the basis of computer based procedure guidelines. It satisfies most requirements such as presentations and functionalities. Besides, SGTR has been performed with ImPRO to evaluate reading comprehension and situation awareness. ImPRO is a software engine which can interpret procedure script language, so that ImPRO is reliable by nature and verified with formal method. One bug, however, had hidden one year after release, but it was fixed. Finally backup paper procedures can be prepared on the same format as VDU in case of ImPRO failure. (authors)

  18. Imaging the Unimaginable: Desorption Electrospray Ionization - Imaging Mass Spectrometry (DESI-IMS) in Natural Product Research.

    Science.gov (United States)

    Parrot, Delphine; Papazian, Stefano; Foil, Daniel; Tasdemir, Deniz

    2018-01-31

    Imaging mass spectrometry (IMS) has recently established itself in the field of "spatial metabolomics." Merging the sensitivity and fast screening of high-throughput mass spectrometry with spatial and temporal chemical information, IMS visualizes the production, location, and distribution of metabolites in intact biological models. Since metabolite profiling and morphological features are combined in single images, IMS offers an unmatched chemical detail on complex biological and microbiological systems. Thus, IMS-type "spatial metabolomics" emerges as a powerful and complementary approach to genomics, transcriptomics, and classical metabolomics studies. In this review, we summarize the current state-of-the-art IMS methods with a strong focus on desorption electrospray ionization (DESI)-IMS. DESI-IMS utilizes the original principle of electrospray ionization, but in this case solvent droplets are rastered and desorbed directly on the sample surface. The rapid and minimally destructive DESI-IMS chemical screening is achieved at ambient conditions and enables the accurate view of molecules in tissues at the µm-scale resolution. DESI-IMS analysis does not require complex sample preparation and allows repeated measurements on samples from different biological sources, including microorganisms, plants, and animals. Thanks to its easy workflow and versatility, DESI-IMS has successfully been applied to many different research fields, such as clinical analysis, cancer research, environmental sciences, microbiology, chemical ecology, and drug discovery. Herein we discuss the present applications of DESI-IMS in natural product research. Georg Thieme Verlag KG Stuttgart · New York.

  19. Evaluation of B1 inhomogeneity effect on DCE-MRI data analysis of brain tumor patients at 3T.

    Science.gov (United States)

    Sengupta, Anirban; Gupta, Rakesh Kumar; Singh, Anup

    2017-12-02

    Dynamic-contrast-enhanced (DCE) MRI data acquired using gradient echo based sequences is affected by errors in flip angle (FA) due to transmit B 1 inhomogeneity (B 1 inh). The purpose of the study was to evaluate the effect of B 1 inh on quantitative analysis of DCE-MRI data of human brain tumor patients and to evaluate the clinical significance of B 1 inh correction of perfusion parameters (PPs) on tumor grading. An MRI study was conducted on 35 glioma patients at 3T. The patients had histologically confirmed glioma with 23 high-grade (HG) and 12 low-grade (LG). Data for B 1 -mapping, T 1 -mapping and DCE-MRI were acquired. Relative B 1 maps (B 1rel ) were generated using the saturated-double-angle method. T 1 -maps were computed using the variable flip-angle method. Post-processing was performed for conversion of signal-intensity time (S(t)) curve to concentration-time (C(t)) curve followed by tracer kinetic analysis (K trans , Ve, Vp, Kep) and first pass analysis (CBV, CBF) using the general tracer-kinetic model. DCE-MRI data was analyzed without and with B 1 inh correction and errors in PPs were computed. Receiver-operating-characteristic (ROC) analysis was performed on HG and LG patients. Simulations were carried out to understand the effect of B 1 inhomogeneity on DCE-MRI data analysis in a systematic way. S(t) curves mimicking those in tumor tissue, were generated and FA errors were introduced followed by error analysis of PPs. Dependence of FA-based errors on the concentration of contrast agent and on the duration of DCE-MRI data was also studied. Simulations were also done to obtain K trans of glioma patients at different B 1rel values and see whether grading is affected or not. Current study shows that B 1rel value higher than nominal results in an overestimation of C(t) curves as well as derived PPs and vice versa. Moreover, at same B 1rel values, errors were large for larger values of C(t). Simulation results showed that grade of patients can change

  20. Expression of the vitamin D-activating enzyme 1α-hydroxylase (CYP27B1) decreases during melanoma progression.

    Science.gov (United States)

    Brożyna, Anna A; Jóźwicki, Wojciech; Janjetovic, Zorica; Slominski, Andrzej T

    2013-03-01

    1α-Hydroxylase (CYP27B1), the enzyme responsible for the synthesis of the biologically active form of vitamin D (1,25(OH)(2)D(3)), is expressed in the skin. To assess the correlation between progression of melanocytic tumors and CYP27B1, we analyzed its expression in 29 benign nevi, 75 primary cutaneous melanomas, 40 metastases, and 4 re-excision and 6 normal skin biopsies. Immunoreactivity for CYP27B1 was significantly lower in the vertical growth phase and metastatic melanomas (0.6 and 0.5 arbitrary units, respectively) in comparison with nevi and radial growth phase tumors (1.2 and 1.1 arbitrary units, respectively); and expression was reduced in more advanced lesions (Clark levels III-V, Breslow thickness ≥2.1 mm; 0.8 and 0.7 arbitrary units, respectively). There was an inverse correlation between CYP27B1 and Ki-67 expression. Furthermore, CYP27B1 expression was reduced in primary melanomas that created metastases in comparison with non-metastasizing melanomas. Reduced CYP27B1 expression in radial growth phase was related to shorter overall survival (810 versus 982 versus 1151 days in melanomas with absent, low, and high CYP27B1 immunoreactivity), and low CYP27B1 expression in radial growth phase and vertical growth phase was related to shorter disease-free survival (114 versus 339 versus 737 days and 129 versus 307 versus 737 days, respectively, in melanomas with absent, low, and high CYP27B1). Also, CYP27B1 expression was inversely related to melanin in melanoma cells in vivo and melanoma cells cultured in vitro. Thus, reduction of CYP27B1 correlates with melanoma phenotype and behavior, and its lack affects the survival of melanoma patients, indicating a role in the pathogenesis and progression of this cancer. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. Could a B-1 cell derived phagocyte "be one" of the peritoneal macrophages during LPS-driven inflammation?

    Directory of Open Access Journals (Sweden)

    Ana Flavia Popi

    Full Text Available The inflammatory response is driven by signals that recruit and elicit immune cells to areas of tissue damage or infection. The concept of a mononuclear phagocyte system postulates that monocytes circulating in the bloodstream are recruited to inflamed tissues where they give rise to macrophages. A recent publication demonstrated that the large increase in the macrophages observed during infection was the result of the multiplication of these cells rather than the recruitment of blood monocytes. We demonstrated previously that B-1 cells undergo differentiation to acquire a mononuclear phagocyte phenotype in vitro (B-1CDP, and we propose that B-1 cells could be an alternative origin for peritoneal macrophages. A number of recent studies that describe the phagocytic and microbicidal activity of B-1 cells in vitro and in vivo support this hypothesis. Based on these findings, we further investigated the differentiation of B-1 cells into phagocytes in vivo in response to LPS-induced inflammation. Therefore, we investigated the role of B-1 cells in the composition of the peritoneal macrophage population after LPS stimulation using osteopetrotic mice, BALB/Xid mice and the depletion of monocytes/macrophages by clodronate treatment. We show that peritoneal macrophages appear in op/op((-/- mice after LPS stimulation and exhibit the same Ig gene rearrangement (VH11 that is often found in B-1 cells. These results strongly suggest that op/op((-/- peritoneal "macrophages" are B-1CDP. Similarly, the LPS-induced increase in the macrophage population was observed even following monocyte/macrophage depletion by clodronate. After monocyte/macrophage depletion by clodronate, LPS-elicited macrophages were observed in BALB/Xid mice only following the transfer of B-1 cells. Based on these data, we confirmed that B-1 cell differentiation into phagocytes also occurs in vivo. In conclusion, the results strongly suggest that B-1 cell derived phagocytes are a component of

  2. Measurement and assessment of aflatoxin B1 and its producing molds in Iranian sausages and burgers

    Directory of Open Access Journals (Sweden)

    Siavash Maktabi

    2016-09-01

    Full Text Available Abstract Introduction: Aflatoxin B1 (AFB1 is one of the most well-known hepatocarcinogens in humans. Contamination of raw materials, used in the production of sausages and burgers, with aflatoxin producing molds can lead to increased level of aflatoxin in the final products and can impose hazards to human health. Unfortunately, aflatoxin is resistant to heating and freezing processes, etc. and can remain in these products untile consumption. Methods: During a six-month period, 45 sausage and 53 burger samples from valid brands across the country were randomly purchased from the stores. The samples were analyzed for AFB1 by ELISA technique. Meanwhile, the number of molds was calculated and aflatoxin producing molds were identified by direct and slide culture methods. Results: The findings showed that 2 susage samples (4.9% and 3 burger samples (6.3% were contaminated with >1 ng/g aflatoxin. Moreover, 4 burger samples (8.9% contaminated with mold included aspergillus flavus, aspergillus niger, mucor, and penicillium while, none of the susage samples showed mold contamination. Conclusion: The Iranian meat products had a relative aflatoxin B1 contamination during the study period, but the contamination rate was low and in allowable range. Standard hygienic preparation and packaging of meat products molds is recommended to reduce fungal contamination, especially aflatoxin-producing molds.

  3. Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts.

    Science.gov (United States)

    Abdel-Wahhab, Mosaad A; Ahmed, Hanaa H; Hagazi, Mohamad M

    2006-01-01

    Chemoprevention by extracts of Laurencia obtusa (E1) and Caulerpa prolifera (E2) collected from the Egyptian coast of the Red Sea against aflatoxin B(1) (AFB(1))-initiated hepatotoxicity in female Sprague-Dawley rats has been studied. Animals were fed aflatoxin-contaminated diet (3 mg kg(-1) diet) for 6 days then treated orally with pure aflatoxin B(1) (AFB(1)) (200 microg kg(-1) b.w.) for 4 days either in combination with or before E1 or E2 administration (50 mg kg(-1) b.w.). AFB(1) resulted in a signicant increase in serum alpha fetoprotein, carcinoembryonic antigen, tumor necrosis factor alpha, nitric oxide, interleukin-1alpha, procollagen III and lipid peroxidation level in the liver. It caused a signicant decrease in food intake, body weight, serum leptin, the activities of glutathione peroxidase, superoxide dismutase and DNA and RNA concentrations in the liver. Cotreatment with AFB(1) and E1 or E2 resulted in an obvious improvement in all tested parameters. Noteworthy, E2 was more effective than E1 in the protection against AFB(1)-induced hepatotoxicity. Copyright 2006 John Wiley & Sons, Ltd.

  4. Phosphorylation of XIAP by CDK1–cyclin-B1 controls mitotic cell death

    Science.gov (United States)

    Hou, Ying; Allan, Lindsey A.

    2017-01-01

    ABSTRACT Regulation of cell death is crucial for the response of cancer cells to drug treatments that cause arrest in mitosis, and is likely to be important for protection against chromosome instability in normal cells. Prolonged mitotic arrest can result in cell death by activation of caspases and the induction of apoptosis. Here, we show that X-linked inhibitor of apoptosis (XIAP) plays a key role in the control of mitotic cell death. Ablation of XIAP expression sensitises cells to prolonged mitotic arrest caused by a microtubule poison. XIAP is stable during mitotic arrest, but its function is controlled through phosphorylation by the mitotic kinase CDK1–cyclin-B1 at S40. Mutation of S40 to a phosphomimetic residue (S40D) inhibits binding to activated effector caspases and abolishes the anti-apoptotic function of XIAP, whereas a non-phosphorylatable mutant (S40A) blocks apoptosis. By performing live-cell imaging, we show that phosphorylation of XIAP reduces the threshold for the onset of cell death in mitosis. This work illustrates that mitotic cell death is a form of apoptosis linked to the progression of mitosis through control by CDK1–cyclin-B1. PMID:27927753

  5. Purification of aflatoxin B1 antibody for the development of aflatoxin biosensor

    Science.gov (United States)

    Prihantoro, E. A. B.; Saepudin, E.; Ivandini, T. A.

    2017-07-01

    Aflatoxin B1 (AFB1) is produced from agricultural products especially peanuts overgrown with aspergillus flavus during the post-harvest process. Aflatoxin is classified as a highly toxic and carcinogenic substance to humans by the International Agency for Research on Cancer (IARC), WHO. This research was conducted to develop the AFB1 biosensor using antibody that specifically binds to aflatoxin B1. This antibody was produced by injecting an AFB1 hapten-protein (immunogen) to a rabbit. Antibody was obtained from rabbit's blood serum and purified using Protein A affinity chromatography and precipitation at the isoelectric point. The result showed that purification using protein A contains antibody of 4.0 mg/mL, whereas purification using precipitation at isoelectric pH contains antibody of 0.3 mg/mL. The pure antibody was tested for its specificity against AFB1, tetrahydrofuran (THF), dimethyl formamide (DMF), bovine serum albumin (BSA), and ethanol. The result revealed that THF, BSA, and ethanol were bound to antibody, while DMF showed no interaction. It was concluded that the polyclonal antibody which have been successfully purified from rabbit's blood serum using protein A affinity chromatography and precipitation methods showed an unspecific identification.

  6. HLA-DQA1/B1 alleles as putative susceptibility markers in congenital toxoplasmosis.

    Science.gov (United States)

    Shimokawa, Paulo Tadashi; Targa, Lília Spaleta; Yamamoto, Lidia; Rodrigues, Jonatas Cristian; Kanunfre, Kelly Aparecida; Okay, Thelma Suely

    2016-05-18

    Host and parasite genotypes are among the factors associated with congenital toxoplasmosis pathogenesis. As HLA class II molecules play a key role in the immune system regulation, the aim of this study was to investigate whether HLA-DQA1/B1 alleles are associated with susceptibility or protection to congenital toxoplasmosis. One hundred and twenty-two fetuses with and 103 without toxoplasmosis were studied. The two study groups were comparable according to a number of socio-demographic and genetic variables. HLA alleles were typed by PCR-SSP. In the HLA-DQA1 region, the allele frequencies showed that *01:03 and *03:02 alleles could confer susceptibility (OR= 3.06, p = 0.0002 and OR= 9.60, p= 0.0001, respectively) as they were more frequent among infected fetuses. Regarding the HLA-DQB1 region, the *05:04 allele could confer susceptibility (OR = 6.95, p HLA-DQA1 susceptibility alleles. In the present study, a discriminatory potential of HLA-DQA1/B1 alleles to identify susceptibility to congenital toxoplasmosis and the most severe cases has been shown.

  7. Tangential Bicortical Locked Fixation Improves Stability in Vancouver B1 Periprosthetic Femur Fractures: A Biomechanical Study.

    Science.gov (United States)

    Lewis, Gregory S; Caroom, Cyrus T; Wee, Hwabok; Jurgensmeier, Darin; Rothermel, Shane D; Bramer, Michelle A; Reid, John Spence

    2015-10-01

    The biomechanical difficulty in fixation of a Vancouver B1 periprosthetic fracture is purchase of the proximal femoral segment in the presence of the hip stem. Several newer technologies provide the ability to place bicortical locking screws tangential to the hip stem with much longer lengths of screw purchase compared with unicortical screws. This biomechanical study compares the stability of 2 of these newer constructs to previous methods. Thirty composite synthetic femurs were prepared with cemented hip stems. The distal femur segment was osteotomized, and plates were fixed proximally with either (1) cerclage cables, (2) locked unicortical screws, (3) a composite of locked screws and cables, or tangentially directed bicortical locking screws using either (4) a stainless steel locking compression plate system with a Locking Attachment Plate (Synthes) or (5) a titanium alloy Non-Contact Bridging system (Zimmer). Specimens were tested to failure in either axial or torsional quasistatic loading modes (n = 3) after 20 moderate load preconditioning cycles. Stiffness, maximum force, and failure mechanism were determined. Bicortical constructs resisted higher (by an average of at least 27%) maximum forces than the other 3 constructs in torsional loading (P steel construct in axial loading. Proximal fixation stability is likely improved with the use of bicortical locking screws as compared with traditional unicortical screws and cable techniques. In this study with a limited sample size, we found the addition of cerclage cables to unicortical screws may not offer much improvement in biomechanical stability of unstable B1 fractures.

  8. Adipocyte Liver Kinase b1 Suppresses Beige Adipocyte Renaissance Through Class IIa Histone Deacetylase 4.

    Science.gov (United States)

    Wang, Yangmeng; Paulo, Esther; Wu, Dongmei; Wu, Yixuan; Huang, Wendong; Chawla, Ajay; Wang, Biao

    2017-12-01

    Uncoupling protein 1 + beige adipocytes are dynamically regulated by environment in rodents and humans; cold induces formation of beige adipocytes, whereas warm temperature and nutrient excess lead to their disappearance. Beige adipocytes can form through de novo adipogenesis; however, how "beiging" characteristics are maintained afterward is largely unknown. In this study, we show that beige adipocytes formed postnatally in subcutaneous inguinal white adipose tissue lost thermogenic gene expression and multilocular morphology at the adult stage, but cold restored their beiging characteristics, a phenomenon termed beige adipocyte renaissance. Ablation of these postnatal beige adipocytes inhibited cold-induced beige adipocyte formation in adult mice. Furthermore, we demonstrated that beige adipocyte renaissance was governed by liver kinase b1 and histone deacetylase 4 in white adipocytes. Although neither presence nor thermogenic function of uncoupling protein 1 + beige adipocytes contributed to metabolic fitness in adipocyte liver kinase b1-deficient mice, our results reveal an unexpected role of white adipocytes in maintaining properties of preexisting beige adipocytes. © 2017 by the American Diabetes Association.

  9. Effect of different dietary fumonisin B1 exposure on the toxin content of porcine tissues

    Directory of Open Access Journals (Sweden)

    Melinda Kovács

    2010-01-01

    Full Text Available The time and dose-dependent effect of fumonisin B1 (FB1 exposure on the tissue toxin concentrations was examined in 36 weaned pigs. Treated animals were fed an experimental diet supplemented with Fusarium verticillioides culture, leading to FB1 daily intake values of 50 mg/animal (n=10 in a 22 day treatment (T50, 100 mg/animal (n=7 for 5 days (T100s, short exposure and 100 mg/animal (n=7 for 10 days (T100l, long exposure, respectively. For each experimental group, 4 control (C animals were used. At the end of trial, the FB1 content of lung, liver, bile, kidney, brain, spleen, pancreas, heart, muscle and fat samples was determined by LC-MS. Pulmonary oedema developed in all piglets as a result of the toxin dose applied. Highest FB1 concentrations were found in the liver, kidney, lung and spleen, in all treatments. The muscle and adipose tissues (i.e. the meat did not contain considerable amounts of fumonisin B1. In the organs of the animals fed 100 mg FB1 per day, significantly higher FB1 levels were measured. An exponential function was descriptive for the measured FB1 data of some organs. The FB1 content was found to be dependent on the average daily intake, except in case of the liver.

  10. Effect of climate change on Aspergillus flavus and aflatoxin B1 production

    Directory of Open Access Journals (Sweden)

    Angel eMedina

    2014-07-01

    Full Text Available This review considers the available information on the potential impact of key environmental factors and their interactions on the molecular ecology, growth and aflatoxin production by Aspergillus flavus in vitro and in maize grain. The recent studies which have been carried out to examine the impact of water activity x temperature on aflatoxin biosynthesis and phenotypic aflatoxin production are examined. These have shown that there is a direct relationship between the relative expression of key regulatory and structural genes under different environmental conditions which correlate directly with aflatoxin B1 production. A model has been developed to integrate the relative expression of 10 biosynthetic genes in the pathway, growth and aflatoxin B1 (AFB1 production which was validated under elevated temperature and water stress conditions. The effect of interacting conditions of aw x temperature x elevated CO2 (2x and 3x existing levels are detailed for the first time. This suggests that while such interacting environmental conditions have little effect on growth they do have a significant impact on aflatoxin biosynthetic gene expression (structural aflD and regulatory aflR genes and can significantly stimulate the production of AFB1. While the individual factors alone have an impact, it is the combined effect of these three abiotic factors which have an impact on mycotoxin production. This approach provides data which is necessary to help predict the real impacts of climate change on mycotoxigenic fungi.

  11. Localization of microbarom sources using the IMS infrasound network

    Science.gov (United States)

    LandèS, Matthieu; Ceranna, Lars; Le Pichon, Alexis; Matoza, Robin S.

    2012-03-01

    Multiyear continuous infrasound monitoring with the global International Monitoring System (IMS) demonstrates that microbaroms, generated by nonlinear interaction of opposing oceanic surface waves, are globally observed at equatorial, middle, and high latitudes. Direction of arrival of microbarom signals are systematically estimated at all IMS infrasound stations. Using a cross-bearing method, we perform a monthly localization of worldwide microbarom sources. At most stations, microbarom detections show clear seasonal trends, driven by stratospheric wind reversals and source effects. Five-year averaging of the detections provides global and statistical observations of seasonal source variations. Our microbarom source locations represent a first attempt to infer ocean swell characteristics from global and long-term measurements. We show that the monthly averaged source locations exhibit clear seasonal switching between the northern and southern hemispheres. Such an approach can help with evaluating the network detection capability, and provides new insights into quantitative relationships between infrasonic observables, atmospheric specifications, and ocean-atmosphere interactions.

  12. reutilizables con XML, UML e IMS-LD

    Directory of Open Access Journals (Sweden)

    José R. Hilera

    2005-01-01

    Full Text Available Se analiza la especificación IMS-Learning Design (LD, que permite el diseño de procesos de enseñanza-aprendizaje en forma de modelos registrados en archivos xml fácilmente reutilizables. Se valora la utilidad del estándar de modelado de sistemas denominado UML (Unified Modeling Language para establecer meta-modelos en la especificación IMS-LD, así como para elaborar modelos dinámicos que reflejen la organización de las actividades de un proceso de enseñanza-aprendizaje. Finalmente, se justifica la necesidad de disponer de herramientas informáticas para el diseño y ejecución de los procesos, de forma similar a como ocurre con los sistemas de workflow.

  13. Geschichtsbezogene und rechtspolitische Polonica im Bücherbestand Gottfried Lengnichs

    Directory of Open Access Journals (Sweden)

    Iwona Imańska

    2017-11-01

    Full Text Available Gottfried Lengnich, der Historiker und Syndikus der Stadt Danzig aus dem 18. Jahrhundert, hinterließ eine Büchersammlung mit über viertausend Büchern, die nach seinem Tode in zwei Auktionen im Juli und November 1774 versteigert werden sollten. Die Auktionskataloge befinden sich in der Staatsbibliothek zu Berlin – Stiftung Preußischer Kulturbesitz und sind auch online zugänglich. Wegen wissenschaftlicher Interessen Lengnichs befanden sich in seiner Bibliothek ca. 600 Polonica-Drucke, die meisten davon waren Abhandlungen im Themenfeld Geschichte, Recht und Politik. Die Analyse dieses Fragments des Bücherbestands Lengnichs bewies, dass der Syndikus eine perfekte Arbeitswerkstatt für sich schuf, indem er die Werke der meisten wichtigsten polnischen und fremden Autoren, die über die Geschichte und die Gesetzgebung Polens und Preußens schrieben, sammelte.

  14. Adaptive Rückmeldungen im intelligenten Tutorensystem LARGO

    Directory of Open Access Journals (Sweden)

    Kevin Ashley

    2009-07-01

    Full Text Available Das intelligente Tutorensystem LARGO für die Rechtswissenschaften soll Jurastudenten helfen, Argumentationsstrategien zu lernen. Im verwendeten Ansatz werden Gerichtsprotokolle als Lernmaterialien verwendet: Studenten annotieren diese und erstellen graphische Repräsentationen des Argumentationsverlaufs. Das System kann dabei zur Reflexion über die von Anwälten vorgebrachten Argumente anregen und Lernende auf mögliche Schwächen in ihrer Analyse des Disputs hinweisen. Zur Erkennung von Schwächen verwendet das System Graphgrammatiken und kollaborative Filtermechanismen. Dieser Artikel stellt dar, wie in LARGO auf Basis der Bestimmung eines „Benutzungskontextes“ die Rückmeldungen im System benutzungsadaptiv gestaltet werden. Weiterhin diskutieren wir auf Basis der Ergebnisse einer kontrollierten Studie mit dem System, welche mit Jurastudierenden an der University of Pittsburgh stattfand, in wie weit der automatisch bestimmte Benutzungskontext zur Vorhersage von Lernerfolgen bei Studenten verwendbar ist.

  15. Estudios por imágenes en reumatismo

    Directory of Open Access Journals (Sweden)

    Muñoz Ch. Sara, Dra.

    2012-07-01

    Los grandes avances tecnológicos de la última década han mejorado la calidad y cantidad de información que las distintas técnicas aportan, especialmente la RM; esto hace vislumbrar un cambio significativo en el diagnóstico por imágenes, ya que en el futuro no solo estará basado en los cambios morfológicos en órganos y tejidos; las imágenes obtenidas aportarán información bioquímica, molecular y fisiológica de los procesos patológicos facilitando su diagnóstico aun más precoz.

  16. 26 CFR 1.509(a)-2 - Exclusion for certain organizations described in section 170(b)(1)(A).

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Exclusion for certain organizations described in....509(a)-2 Exclusion for certain organizations described in section 170(b)(1)(A). (a) General rule. Organizations described in section 170(b)(1)(A) (other than in clauses (vii) and (viii)) are excluded from the...

  17. Lipoprotein profiles in human heterozygote carriers of a functional mutation P297S in scavenger receptor class B1

    NARCIS (Netherlands)

    Ljunggren, Stefan A.; Levels, Johannes H. M.; Hovingh, Kees; Holleboom, Adriaan G.; Vergeer, Menno; Argyri, Letta; Gkolfinopoulou, Christina; Chroni, Angeliki; Sierts, Jeroen A.; Kastelein, John J.; Kuivenhoven, Jan Albert; Lindahl, Mats; Karlsson, Helen

    2015-01-01

    The scavenger receptor class B type 1 (SR-B1) is an important HDL receptor involved in cholesterol uptake and efflux, but its physiological role in human lipoprotein metabolism is not fully understood. Heterozygous carriers of the SR-B1(P297S) mutation are characterized by increased HDL cholesterol

  18. 26 CFR 1.673(b)-1 - Income payable to charitable beneficiaries before amendment by Tax Reform Act of 1969).

    Science.gov (United States)

    2010-04-01

    ... before amendment by Tax Reform Act of 1969). 1.673(b)-1 Section 1.673(b)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Grantors and... amendment by Tax Reform Act of 1969). (a) Pursuant to section 673(b) a grantor is not treated as an owner of...

  19. Increased susceptibility to atrial fibrillation secondary to atrial fibrosis in transgenic goats expressing transforming growth factor - B1

    Science.gov (United States)

    Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia in people with significant morbidity and mortality. There is a strong association between atrial fibrosis and AF. Transforming growth factor B1 (TGF-B1) is an essential mediator of atrial fibrosis in animal models and human pat...

  20. KINETICS OF PERITONEAL B-1A CELLS (CD5 B-CELLS) IN YOUNG-ADULT MICE

    NARCIS (Netherlands)

    DEENEN, GJ; KROESE, FGM

    In the mouse, conventional B cells are continuously generated from precursor cells located in the bone marrow (BM), whereas the small subset of B-1 cells (formerly called Ly-1 B cells) constitute a self-replenishing population of cells. Here we studied the kinetics of murine peritoneal B-1a cells

  1. 26 CFR 1.1092(b)-1T - Coordination of loss deferral rules and wash sale rules (temporary).

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 11 2010-04-01 2010-04-01 true Coordination of loss deferral rules and wash sale rules (temporary). 1.1092(b)-1T Section 1.1092(b)-1T Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Wash Sales of Stock Or Securities...

  2. The impact of SF3B1 mutations in CLL on the DNA-damage response

    DEFF Research Database (Denmark)

    Te Raa, G D; Derks, I A M; Navrkalova, V

    2015-01-01

    . Interestingly, SF3B1 mutated samples without concurrent ATM and TP53 aberrations (sole SF3B1) displayed partially defective ATM/p53 transcriptional and apoptotic responses to various DNA-damaging regimens. In contrast, NOTCH1 or K/N-RAS mutated CLL displayed normal responses in p53/ATM target gene induction...

  3. Rescue of defective ATP8B1 trafficking by CFTR correctors as a therapeutic strategy for familial intrahepatic cholestasis

    NARCIS (Netherlands)

    van der Woerd, Wendy L.; Wichers, Catharina G. K.; Vestergaard, Anna L.; Andersen, Jens Peter; Paulusma, Coen C.; Houwen, Roderick H. J.; van de Graaf, Stan F. J.

    Background & Aims ATP8B1 deficiency is an autosomal recessive liver disease characterized by intrahepatic cholestasis. ATP8B1 mutation p.I661T, the most frequent mutation in European patients, results in protein misfolding and impaired targeting to the plasma membrane. Similarly, mutations in cystic

  4. Rescue of defective ATP8B1 trafficking by CFTR correctors as a therapeutic strategy for familial intrahepatic cholestasis

    NARCIS (Netherlands)

    van der Woerd, Wendy L.; Wichers, Catharina G. K.; Vestergaard, Anna L.; Andersen, Jens Peter; Paulusma, Coen C.; Houwen, Roderick H. J.; van de Graaf, Stan F. J.

    2016-01-01

    ATP8B1 deficiency is an autosomal recessive liver disease characterized by intrahepatic cholestasis. ATP8B1 mutation p.I661T, the most frequent mutation in European patients, results in protein misfolding and impaired targeting to the plasma membrane. Similarly, mutations in cystic fibrosis

  5. Collecting Virtual Reference Statistics with an IM Chat-Bot

    Directory of Open Access Journals (Sweden)

    Mason R.K. Hall

    2008-06-01

    Full Text Available A perennial problem in libraries is capturing accurate statistics. This article addresses this problem with the creative use of Web 2.0 tools: Meebo and AOL Instant Messenger. It describes the development and implementation of an instant messaging "stat-bot" that prompts staff to record virtual reference statistics via IM. Step-by-step guidelines and the perl script are provided.

  6. Recent research work resulting in IMS building technology improvements

    Directory of Open Access Journals (Sweden)

    Goran PETROVIĆ

    2013-12-01

    Full Text Available IMS Building Technology is based on pre-fabricated concrete elements of the structure, assembled on-site and joined using prestressing. This construction method, developed in 1950s and implemented worldwide, is still in use. This paper describes recent improvements and the research work that initiated and enabled them, as well as on-site experiences from the process of implementation.

  7. Synthesis of Cyclic Py-Im Polyamide Libraries

    OpenAIRE

    Li, Benjamin C.; Montgomery, David C.; Puckett, James W.; Dervan, Peter B.

    2013-01-01

    Cyclic Py-Im polyamides containing two GABA turn units exhibit enhanced DNA binding affinity, but extensive studies of their biological properties have been hindered due to synthetic inaccessibility. A facile modular approach toward cyclic polyamides has been developed via microwave-assisted solid-phase synthesis of hairpin amino acid oligomer intermediates followed by macrocyclization. A focused library of cyclic polyamides 1–7 targeted to the androgen response element (ARE) and the estrogen...

  8. Lösungspfadbasierte Analysen im Entwurf komplexer Systeme

    OpenAIRE

    Bölling, Michael

    2015-01-01

    Durch den Einsatz graphenbasierter Entwurfssprachen im Entwurf komplexer Systeme entstehen zunehmend größere und komplexere funktionale Modellbeschreibungen in Form nicht-linearer algebraischer Gleichungssysteme. Diese machen es dem Entwerfer immer schwieriger, die wesentlichen Einflussgrößen eines Modells, sogenannte Design Driver, zu erkennen und die Auswirkungen einzelner Parameter samt deren Kopplungen über das gesamte System hinweg richtig einzuschätzen. Die manuelle Analyse der Gleic...

  9. Expertise bewerben und finden im Social Semantic Web

    Science.gov (United States)

    Polleres, Axel; Mochol, Malgorzata

    Im vorliegenden Beitrag diskutieren wir Rahmenbedingungen zur Kombination, Wiederverwendung und Erweiterung bestehender RDFVokabulare im Social Semantic Web. Hierbei konzentrieren wir uns auf das Anwendungsszenario des Auffindens und Bewerbens von Experten im Web oder Intranet. Wir präsentieren, wie RDF-Vokabulare einerseits und de facto Standardformate andererseits, die von täglich verwendeten Applikationen benutzt werden (z. B. vCard, iCal oder Dublin Core), kombiniert werden können, um konkrete Anwendungsfälle der Expertensuche und zum Management von Expertise zu lösen. Unser Fokus liegt darauf aufzuzeigen, dass für praktische Anwendungsszenarien nicht notwendigerweise neue Ontologien entwickelt werden müssen, sondern der Schlüssel vielmehr in der Integration von bestehenden, weit verbreiteten und sich ergänzenden Formaten zu einem kohärenten Netzwerk von Ontologien liegt. Dieser Ansatz garantiert sowohl direkte Anwendbarkeit von als auch niedrige Einstiegsbarrieren in Semantic Web-Technologien sowie einfache Integrierbarkeit in bestehende Applikationen. Die im Web verfügbaren und verwendeten RDFFormate decken zwar einen großen Bereich der Aspekte zur Beschreibung von Personen und Expertisen ab, zeigen aber auch signifikante Überlappungen. Bisher gibt es wenig systematische Ansätze, um diese Vokabulare zu verbinden, sei es in Form von allgemeingültigen Praktiken, die definieren, wann welches Format zu benutzen ist, oder in Form von Regeln, die Überlappungen zwischen einzelnen Formaten formalisieren. Der vorliegende Artikel analysiert, wie bestehende Formate zur Beschreibung von Personen, Organisationen und deren Expertise kombiniert und, wo nötig, erweitert werden können. Darüber hinaus diskutieren wir Regelsprachen zur Beschreibung von Formatüberlappungen sowie deren praktische Verwendbarkeit zur Erstellung eines Ontologie-Netzwerks zur Beschreibung von Experten.

  10. Business-Intelligence-Systeme im Spannungsfeld zwischen Usability und Sicherheit

    OpenAIRE

    Hinck, Thorsten

    2010-01-01

    Business-Intelligence-Systeme stehen im Spannungsfeld zwischen Usability und Sicherheit. Die Gefahren und Sicherheitsmaßnahmen für Informationssysteme werden ermittelt und ökonomisch in Bezug zur Gebrauchstauglichkeit gesetzt. Es entsteht ein Sicherheitsprofil für Business-Intelligence-Systeme. Business-Intelligence-Systems are in tension between Usability and IT-Security. Risks and safety measures of informationsystems are defined and set in (economic) regard to Usability. A security prof...

  11. Privacidad en imágenes jpg mediante XACML

    OpenAIRE

    Durán Montoro, Alberto

    2016-01-01

    Este trabajo propone una solución a la pérdida de privacidad de las imágenes JPEG mediante la inclusión de políticas de privacidad. Posteriormente se genera una petición de acceso a la imagen y un autorizador determina si se tienen o no privilegios para visualizar el contenido de la imagen.

  12. Mediengestaltung im interkulturellen Vergleich. Eine Studie zu Mikroaspekten der Globalisierung

    OpenAIRE

    Vera-Stahl, Gilda

    2017-01-01

    Die vorliegende Arbeit befasst sich mit dem interkulturellen Vergleich von lokalen Werbespots aus Deutschland und Ecuador. Im Zentrum des Interesses steht die implizite Dimension der technisch gebundenen audiovisuellen Kommunikation, d.h. die Art und Weise, wie die Kommunikationsmedien – das Bild, die Sprache und die Musik – bei der Gestaltung der Spots verwendet werden. Ausgangspunkt der Untersuchung bildet der Ansatz von Nothnagel/Vera (2004, 2005), dass Habitusformen der Face-to-Face-...

  13. Untersuchungen zur Rolle der Proteintyrosinphosphatase PTPα im Mammakarzinomsystem

    OpenAIRE

    Treder, Martin

    2008-01-01

    Ziel der vorliegenden Arbeit war es die Rolle der PTPα im Mammakarzinomsystem zu untersuchen, da eine mögliche Beteiligung dieser Phosphatase an der Förderung der neoplastischen Transformation oder der Fortschreitung der Brusttumoren nicht beschrieben wurde. In einem molekulardiagnostischen Teil konnte mittels cDNA-Arrays eine klare hoch-regulierte Expression der PTPα in primären Brusttumoren und in Brustkrebszellinien gezeigt werden. Eine starke Expressionskorrelation zwischen PTPα und c-src...

  14. Frauen mit Eßstörungen im Ausdauersport

    OpenAIRE

    Bös, Annette

    2003-01-01

    Die vorliegende Arbeit untersucht das Phänomen Eßstörungen bei Ausdauersportlerinnen. Auf dem Hintergrund des identitätstheoretischen Ansatzes von Stahr et al. (1995) geht es um die Frage nach den Hauptverursachungsmomenten der Eßstörungen von sieben jungen Sportlerinnen. Im Rahmen einer qualitativen Sozialforschungsarbeit wurden sieben Athletinnen in einem problemzentrierten Interview zur Phase ihrer Eßstörungserkrankung befragt. Die Arbeit setzt sich zum Ziel, einen Beitrag zur systematisch...

  15. QUARTERLY TECHNICAL REPORT FOR IN-MINE (IM) SYSTEM

    International Nuclear Information System (INIS)

    Zvi H. Meiksin

    2001-01-01

    A circuit that had been earlier lab-tested to eliminate multi-antenna interference in the In-mine (IM) system was fabricated, implemented and tested successfully in a system setting. An adaptive, tracking comb-filter for the through-the-earth (TTE) communications system was designed and implemented. This resulted in noticeable noise reduction. Studies for multi-channel transmission have begun

  16. The coupling between the IAS and the IMS

    Energy Technology Data Exchange (ETDEWEB)

    Bes, D.R.; Civitarese, O. E-mail: civitare@fisica.unlp.edu.ar

    2004-09-06

    In a previous paper we have studied the structure of the Isobaric Analogue State (IAS) using a realistic single-particle model within a formalism that allows to disentangle real effects of isospin violations from spurious effects associated with the construction of basis states. In this work we discuss the influence of the Isovector Monopole State (IMS) on the width of the IAS. According to our results, the presence of a collective IMS is necessary to preserve the empirically found isolation of the IAS from states of the background. This is valid for a simplified harmonic oscillator model and for a realistic single-particle basis as well, since for both cases there is a strong Coulomb mixing between the IAS and the IMS, which increases at the expense of decreasing the mixing between the IAS and the {delta}N=0 configuration states. The prediction of the total width of the IAS remains in reasonable agreement with the experimental value in a calculation without free parameters.

  17. Information management in the transport sector; Informationsmanagement im Verkehr

    Energy Technology Data Exchange (ETDEWEB)

    Daduna, J.R. [Fachhochschule fuer Wirtschaft, Berlin (Germany); Voss, S. [eds.] [Technische Univ. Braunschweig (Germany). Abt. Allgemeine Betriebswirtschaftslehre, Wirtschaftsinformatik und Informationsmanagement

    2000-07-01

    If both mobility and space for living are to be assured in Europe during the 21st century, concepts must be developed which make mobility both economically and ecologically acceptable. The book provides an introduction to information management in transportation. It is based on a model which views information management at different levels. On this basis, supporting applications of information and communication technologies can be formulated on the basis of economic and decision-oriented requirements. The book focuses on the necessary functionalities of these applications and the necessary infrastructure. Practical examples are presented from the fields of public transport, individual motor car transport and cargo transport. [German] Sollen Mobilitaet und Lebensraum im Europa des 21. Jahrhunderts erhalten bleiben, so sind Konzepte gefordert, die einen Beitrag zur wirtschaftlichen und oekologisch vertretbaren Mobilitaetsbewaeltigung leisten. Das Buch bietet hierzu einen umfassenden Einstieg in das Informationsmanagement im Verkehr. Ausgehend von Ueberlegungen zu einem Ebenenmodell des Informationsmanagements lassen sich aus betriebswirtschaftlichen und entscheidungsorientierten Anforderungen Unterstuetzungsleistungen ableiten, die unter Vewendung geeigneter Informations- und Kommunikationstechnologien zu erbringen sind. Im Vordergrund der Betrachtungen stehen die zu fordernden Funktionalitaeten an entsprechende Applikationen und die hierfuer benoetigte Infrastruktur. Anhand von Beispielen aus der Praxis werden Anwendungen aus den Bereichen oeffentlicher Personenverkehr, motorisierter Individualverkehr und Gueterverkehr vorgestellt. (orig.)

  18. ImSET: Impact of Sector Energy Technologies

    Energy Technology Data Exchange (ETDEWEB)

    Roop, Joseph M.; Scott, Michael J.; Schultz, Robert W.

    2005-07-19

    This version of the Impact of Sector Energy Technologies (ImSET) model represents the ''next generation'' of the previously developed Visual Basic model (ImBUILD 2.0) that was developed in 2003 to estimate the macroeconomic impacts of energy-efficient technology in buildings. More specifically, a special-purpose version of the 1997 benchmark national Input-Output (I-O) model was designed specifically to estimate the national employment and income effects of the deployment of Office of Energy Efficiency and Renewable Energy (EERE) -developed energy-saving technologies. In comparison with the previous versions of the model, this version allows for more complete and automated analysis of the essential features of energy efficiency investments in buildings, industry, transportation, and the electric power sectors. This version also incorporates improvements in the treatment of operations and maintenance costs, and improves the treatment of financing of investment options. ImSET is also easier to use than extant macroeconomic simulation models and incorporates information developed by each of the EERE offices as part of the requirements of the Government Performance and Results Act.

  19. Imágenes y apocaliptismo en el Occidente medieval

    Directory of Open Access Journals (Sweden)

    Sanmartín, Israel

    2011-12-01

    Full Text Available This article will explore the importance of imagery in the medieval world and particularly in some representations related to eschatological events related to apocalypticism. We´ll work separately the images in the medieval world and a brief development of apocalypticism. The ultimate stage of the article will seek opportunities for dialogue between images and apocalypticism, which lead us to reflect on different iconography and apocalyptic. We ended withthe importance of “location” of the so-called object-images in relation to its function of making the absent and present practice of producing real.

    En el presente artículo se estudiará la importancia de las imágenes en el mundo medieval y en concreto en algunas representaciones vinculadas a episodios escatológicos vinculados al apocaliptismo. Estudiaremos de forma separada tanto la imagen en el mundo medieval como un breve desarrollo del apocaliptismo. El fin último del trabajo será buscar los espacios de encuentro entre las imágenes y el apocaliptismo, que nos llevarán a reflexionar sobre diferentes iconografías apocalípticas y concluir en la importancia de la “localización” de las llamadas imágenes-objeto en relación a su función de hacer presente lo ausente y ejercer de productoras de lo real.

  20. [Content of free and bound thiamine diphosphate in the liver hyaloplasm of vitamine B1 deficient rats].

    Science.gov (United States)

    Ostrovskiĭ, Iu M; Voskoboev, A I; Gritsenko, E A; Grushnik, V V

    1979-01-01

    The amount of free and protein-bound thiamin diphosphate (TDP) in the liver hyaloplasm of B1 vitamin deficient rats has been measured. In the norm the content of protein-bound TDP remains stable (4.5--4.7 micrograms/g tissue) and does not grow upon thiamin injections. The level of the free coenzyme varies appreciably: in the B1-avitaminotic state the content of free TDP decreases, and in the B1-saturated condition it may exceed the norm 4 times. In the liver this enzyme occurs only as a holoenzyme. In case of B1 vitamin deficiency in the diet the transketolase apoform cannot be detected in the liver. A new model for rapid generation of B1-avitaminosis characterized by a significantly lower level of free and bound TDP is described.

  1. Effects of Cobalt on Manganese Oxidation by Pseudomonas putida MnB1

    Science.gov (United States)

    Pena, J.; Bargar, J.; Sposito, G.

    2005-12-01

    The oxidation of Mn(II) in the environment is thought to occur predominantly through biologically mediated pathways. During the stationary phase of growth, the well-characterized freshwater and soil bacterium Pseudomonas putida MnB1 oxidizes soluble Mn(II) to a poorly crystalline layer type Mn(IV) oxide. These Mn oxide particles (2 - 5 nm thickness) are deposited in a matrix of extracellular polymeric substances (EPS) surrounding the cell, creating a multi-component system distinct from commonly studied synthetic Mn oxides. Accurate characterization of the reactivity of these biomineral assemblages is essential to understanding trace metal biogeochemistry in natural waters and sediments. Moreover, these biogenic oxides may potentially be used for the remediation of surface and ground waters impacted by mining, industrial pollution, and other anthropogenic activities. In this study, we consider the interactions between Co, P. putida MnB1, and its biogenic Mn oxide. Cobalt is a redox-active transition metal which exists in the environment as Co(II) and Co(III). While Co is not generally found in the environment at toxic concentrations, it may be released as a byproduct of mining activities (e.g. levels of up to 20 μM are found in Pinal Creek, AZ, a stream affected by copper mining). In addition, the radionuclide 60Co, formed by neutron activation in nuclear reactors, is of concern at Department of Energy sites, such as that at Hanford, and has several industrial applications, including radiotherapy. We address the following questions: Do high levels of Co inhibit enzymatic processes such as Mn(II) oxidation? Can the multicopper oxidase enzyme involved in Mn(II) oxidation facilitate Co(II) oxidation? Lastly, does the organic matter surrounding the oxides affect Co or Mn oxide reactivity? These issues were approached via wet chemical analysis, synchrotron radiation X-ray diffraction (SR-XRD), and extended X-ray absorption fine structure (EXAFS) spectroscopy. In the

  2. T-cell-independent immune responses do not require CXC ligand 13-mediated B1 cell migration.

    Science.gov (United States)

    Colombo, Matthew J; Sun, Guizhi; Alugupalli, Kishore R

    2010-09-01

    The dynamic movement of B cells increases the probability of encountering specific antigen and facilitates cell-cell interactions required for mounting a rapid antibody response. B1a and B1b cells are enriched in the coelomic cavity, contribute to T-cell-independent (TI) antibody responses, and increase in number upon antigen exposure. B1 cell movement is largely governed by Cxc ligand 13 (Cxcl13), and mice deficient in this chemokine have a severe reduction in peritoneal B1 cells. In this study, we examined the role of Cxcl13-dependent B cell migration using Borrelia hermsii infection or intraperitoneal immunization with pneumococcal polysaccharide or 4-hydroxy-3-nitrophenyl-acetyl (NP)-Ficoll, all of which induce robust antibody responses from B1b cells. Surprisingly, we found that antibody responses to B. hermsii or to FhbA, an antigenic target of B1b cells, and the resolution of bacteremia were indistinguishable between wild-type and Cxcl13-/- mice. Importantly, we did not observe an expansion of peritoneal B1b cell numbers in Cxcl13-/- mice. Nonetheless, mice that had resolved infection were resistant to reinfection, indicating that the peritoneal B1b cell reservoir is not required for controlling B. hermsii. Furthermore, despite a reduced peritoneal B1b compartment, immunization with pneumococcal polysaccharide vaccine yielded comparable antigen-specific antibody responses in wild-type and Cxcl13-/- mice and conferred protection against Streptococcus pneumoniae. Likewise, immunization with NP-Ficoll elicited similar antibody responses in wild-type and Cxcl13-/- mice. These data demonstrate that homing of B1 cells into the coelomic cavity is not a requirement for generating protective TI antibody responses, even when antigen is initially localized to this anatomical compartment.

  3. Immunohistochemical Expression of Cyclin B1 in Epithelial Hyperplasia, Dysplasia and Oral Squamous Cell Carcinomas - A Comparative Study.

    Science.gov (United States)

    Niranjan, Kochli Channappa; Tayaar, Amsavardani; Kumar, G S; Krishnapillai, Rekha; Hallikeri, Kaveri; Hunasgi, Santosh

    2016-09-01

    Cyclin B1 is important in the cell cycle progression from G2 to M phase. Cyclin B1 binds to CDC2, which then becomes dephosphorylated and gets relocated to the nucleus, ensuring the transition toward mitosis. Over expression of Cyclin B1, has been reported more recently in breast, colon, prostate, oral and esophageal carcinomas. Thus, the aim of the present study was to examine the expression of Cyclin B1 in hyperplasia, dysplasia and Oral Squamous Cell Carcinomas (OSCC). A total of 64 histopathologically diagnosed cases of epithelial hyperplasias, dysplastic oral epithelium and OSCC were included in the study. Immunohistochemical procedure was carried out using the monoclonal mouse Cyclin B1 antibody (Clone V-152). The Cyclin B1 positive tumor cells counted were expressed as percentage of positive tumor cells. Nuclear and cytoplasmic labeling index (n&cLI) were calculated. The results were tabulated and statistically analyzed by Kruskal Wallis test- One Way ANOVA and Mann Whitney U- test. Combined n&cLI was considered only in 28.57% of epithelial hyperplasias, 40.7% of oral epithelial dysplasias and 72% of OSCC showed over expression of Cyclin B1 with p value being 0.029. Cyclin B1 expression was not significantly different between the grades of dysplasia, between the grades of OSCC and between the marginal groups. The present study demonstrates more than 50% of the study group showing less than 20% of nuclear staining. The importance of such variations within a type of lesion requires further investigation, since Cyclin B1 has proved useful in many studies from esophageal and laryngeal squamous cell carcinoma as a prognostic indicator, an indicator of recurrence and as an indicator for tumor sensitivity to radiotherapy. Further studies are to be extended towards evaluating the role of Cyclin B1 as a prognostic indicator.

  4. A novel inflammatory pathway involved in leukocyte recruitment: role for the kinin B1 receptor and the chemokine CXCL5

    Science.gov (United States)

    Duchene, Johan; Lecomte, Florence; Ahmed, Saleh; Cayla, Cecile; Pesquero, Joao; Bader, Michael; Perretti, Mauro; Ahluwalia, Amrita

    2013-01-01

    The kinin B1 receptor is an inducible receptor not normally expressed but induced by inflammatory stimuli and plays a major role in neutrophil recruitment, particularly in response to the cytokine interleukin-1β (IL-1β). However, the exact mechanism involved in this response is unclear. The aim of this study was to dissect the molecular mechanism involved, in particular to determine whether specific ELR-CXCL chemokines (specific neutrophil chemoattractants) played a role. Using intravital microscopy, we demonstrated that IL-1 β induced leukocyte rolling, adherence and emigration in mesenteric venules of wild type (WT) mice, associated with an increase of B1 receptor mRNA expression, was substantially attenuated (>80%) in B1 receptor knockout mice (B1KO). This effect in B1KO mice was correlated with a selective down regulation of IL-1β-induced CXCL5 mRNA and protein expression compared to WT mice. Furthermore a selective neutralizing CXCL5 antibody caused profound suppression of leukocyte emigration in IL-1β treated WT mice. Finally, treatment of human endothelial cells with IL-1β enhanced mRNA expression of B1 receptor and the human CXCL5 homologues (hCXCL5 and hCXCL6). This response was suppressed by ~50% when cells were pretreated with the B1 receptor antagonist des-Arg9[Leu]8BK whilst treatment with des-Arg9-BK, the B1 receptor agonist, caused a concentration-dependent increase in hCXCL5 and hCXCL6 mRNA expression. This study unveils a pro-inflammatory pathway centred on kinin B1 receptor activation of CXCL5 leading to leukocyte trafficking, and highlights the B1 receptor as a potential target in the therapeutics of inflammatory disease. PMID:17878384

  5. Vitamin B1 in marine sediments: pore water concentration gradient drives benthic flux with potential biological implications

    Directory of Open Access Journals (Sweden)

    Danielle eMonteverde

    2015-05-01

    Full Text Available Vitamin B1, or thiamin, can limit primary productivity in marine environments, however the major marine environmental sources of this essential coenzyme remain largely unknown. Vitamin B1 can only be produced by organisms that possess its complete synthesis pathway, while other organisms meet their cellular B1 quota by scavenging the coenzyme from exogenous sources. Due to high bacterial cell density and diversity, marine sediments could represent some of the highest concentrations of putative B1 producers, yet these environments have received little attention as a possible source of B1 to the overlying water column. Here we report the first dissolved pore water profiles of B1 measured in cores collected in two consecutive years from Santa Monica Basin, CA. Vitamin B1 concentrations were fairly consistent between the two years ranging from 30 pM up to 770 pM. A consistent maximum at ~5 cm sediment depth covaried with dissolved concentrations of iron. Pore water concentrations were higher than water column levels and represented some of the highest known environmental concentrations of B1 measured to date, (over two times higher than maximum water column concentrations suggesting increased rates of cellular production and release within the sediments. A one dimensional diffusion-transport model applied to the B1 profile was used to estimate a diffusive benthic flux of ~0.7 nmol m 2 d-1. This is an estimated flux across the sediment-water interface in a deep sea basin; if similar magnitude B-vitamin fluxes occur in shallow coastal waters, benthic input could prove to be a significant B1-source to the water column and may play an important role in supplying this organic growth factor to auxotrophic primary producers.

  6. Cholesterol Sulfonation Enzyme, SULT2B1b, Modulates AR and Cell Growth Properties in Prostate Cancer.

    Science.gov (United States)

    Vickman, Renee E; Crist, Scott A; Kerian, Kevin; Eberlin, Livia; Cooks, R Graham; Burcham, Grant N; Buhman, Kimberly K; Hu, Chang-Deng; Mesecar, Andrew D; Cheng, Liang; Ratliff, Timothy L

    2016-09-01

    Cholesterol accumulates in prostate lesions and has been linked to prostate cancer incidence and progression. However, how accumulated cholesterol contributes to prostate cancer development and progression is not completely understood. Cholesterol sulfate (CS), the primary sulfonation product of cholesterol sulfotransferase (SULT2B1b), accumulates in human prostate adenocarcinoma and precancerous prostatic intraepithelial neoplasia (PIN) lesions compared with normal regions of the same tissue sample. Given the enhanced accumulation of CS in these lesions, it was hypothesized that SULT2B1b-mediated production of CS provides a growth advantage to these cells. To address this, prostate cancer cells with RNAi-mediated knockdown (KD) of SULT2B1b were used to assess the impact on cell growth and survival. SULT2B1b is expressed and functional in a variety of prostate cells, and the data demonstrate that SULT2B1b KD, in LNCaP and other androgen-responsive (VCaP and C4-2) cells, results in decreased cell growth/viability and induces cell death. SULT2B1b KD also decreases androgen receptor (AR) activity and expression at mRNA and protein levels. While AR overexpression has no impact on SULT2B1b KD-mediated cell death, the addition of exogenous androgen is able to partially rescue the growth inhibition induced by SULT2B1b KD in LNCaP cells. These results suggest that SULT2B1b positively regulates the AR either through alterations in ligand availability or by interaction with critical coregulators that influence AR activity. These findings provide evidence that SULT2B1b is a novel regulator of AR activity and cell growth in prostate cancer and should be further investigated for therapeutic potential. Mol Cancer Res; 14(9); 776-86. ©2016 AACR. ©2016 American Association for Cancer Research.

  7. Tingiv kõneviis eesti B1- ja B2-taseme kirjalikus õppijakeeles kui keeleoskuse arengu näitaja

    Directory of Open Access Journals (Sweden)

    Mare

    2015-10-01

    Full Text Available Kirjeldan artiklis tingiva kõneviisi kasutamist eesti keele kui teise keele täiskasvanud õppijate B1- ja B2-taseme kirjalikes eksamitekstides. Artikli aluseks olnud uuring on osa suuremast uuringust, mille eesmärk on luua Euroopa Keeleõppe Raamdokumendi (CEFR 2007 B1- ja B2-keeleoskustaseme funktsionaalsete keelekirjelduste juurde lingvistilised kirjeldused. Selleks on kavas leida mõlemal tasemel tüüpiliselt kasutatavad lingvistilised kategooriad, kirjeldada nende kasutuse sagedust, analüüsida kategooriate keerukust ja täpsust (Housen jt 2012, leida nende seosed suhtlusfunktsioonidega ning tuua välja tasemeid eristavad deskriptorid. Et tingiva kõneviisi kasutussagedus on B2-tasemel võrreldes B1-tasemega palju suurem (Kitsnik 2014, on tingiv kõneviis üks B1- ja B2-taset eristav kategooria, mis vajab detailsemat uurimist. Artiklis kajastatava uuringu tulemused näitavad, et nii B1- kui ka B2-tasemel kasutatakse ainult tingiva kõneviisi isikulise tegumoe olevikuvorme. B1-tasemel esineb tingiv kõneviis koos kaheksa eri verbiga, neist sagedamini kahe verbiga, milleks on tahtma ja saama. B2-tasemel esineb tingiv kõneviis 23 eri verbiga, neist sagedamini kuue verbiga: tahtma, olema, võima, pidama, soovima, soovitama. B2-tasemel kasutatakse tingivat kõneviisi lisaks B1-tasemel esinenud ainsuse esimese ja kolmanda pöörde vormidele ka teistes morfoloogilistes vormides ning lisaks B1-tasemel esinenud konstruktsioonile “verb tingivas kõneviisis + infinitiiv” ka teistes konstruktsioonides (objektiga, öeldistäitega ja liitlause pea- ning kõrvallauses. Suhtlusfunktsioonidest kasutatakse tingivat kõneviisi nii B1- kui ka B2-tasemel peamiselt viisakuse väljendamiseks. Keeleline täpsus suureneb B2-tasemel neis konstruktsioonides, mis on kasutusel ka B1-tasemel.

  8. Detection of tetrahydrocannabinol residues on hands by ion-mobility spectrometry (IMS). Correlation of IMS data with saliva analysis.

    Science.gov (United States)

    Sonnberg, Saskia; Armenta, Sergio; Garrigues, Salvador; de la Guardia, Miguel

    2015-08-01

    Ion-mobility spectroscopy (IMS) was evaluated as a high-throughput, cheap, and efficient analytical tool for detecting residues of tetrahydrocannabinol (THC) on hands. Regarding the usefulness of hand residues as potential samples for determining THC handling and abuse, we studied the correlation between data obtained from cannabis consumers who were classified as positive after saliva analysis and from those who were classified as positive on the basis of the information from hand-residue analysis. Sampling consisted of wiping the hands with borosilicate glass microfiber filters and introducing these directly into the IMS after thermal desorption. The possibility of false positive responses, resulting from the presence of other compounds with a similar drift time to THC, was evaluated and minimised by applying the truncated negative second-derivative algorithm. The possibility of false negative responses, mainly caused by competitive ionisation resulting from nicotine, was also studied. Graphical abstract THC residues: from hands to analytical signals.

  9. [Das Bild und die Wahrnehmung der Stadt und der städtischen Gesellschaft im Hanseraum im Mittelalter und in der frühen Neuzeit] / Juhan Kreem

    Index Scriptorium Estoniae

    Kreem, Juhan

    2009-01-01

    Arvustus: Das Bild und die Wahrnehmung der Stadt und der städtischen Gesellschaft im Hanseraum im Mittelalter und in der frühen Neuzeit. hrsg. v. Roman Czaja. Torun, 2004. Torunis 2002. aastal toimunud konverentsi materjalid. Projekti "Pilt ja linn" raames ilmunud publikatsioonide loendit saab näha aadressil http://www.historiaurbium.org/english/attivita_images_en.html.

  10. Johannes-Dieter Steinert. Deportation und Zwangsarbeit. Polnische und sowjetische Kinder im nationalsozialistischen Deutschland und im besetzten Osteuropa 1939–1945.

    Directory of Open Access Journals (Sweden)

    Mark Edele

    2015-01-01

    Full Text Available Johannes-Dieter Steinert. Deportation und Zwangsarbeit. Polnische und sowjetische Kinder im nationalsozialistischen Deutschland und im besetzten Osteuropa 1939–1945. [Deportation and Forced Labour. Polish and Soviet Children in Nazi Germany and Occupied Eastern Europe 1939-1945] Essen: Klartext Verlag, 2013. 306 pp. Bibliography. Paper.

  11. Receptor oligomerization in family B1 of G-protein-coupled receptors

    DEFF Research Database (Denmark)

    Roed, Sarah Norklit; Ørgaard, Anne; Jørgensen, Rasmus

    2012-01-01

    , investigation of family B1 receptor oligomerization and especially its pharmacological importance is still at an early stage. Even though GPCR oligomerization is a well-established phenomenon, there is a need for more investigations providing a direct link between these interactions and receptor functionality......The superfamily of the seven transmembrane G-protein-coupled receptors (7TM/GPCRs) is the largest family of membrane-associated receptors. GPCRs are involved in the pathophysiology of numerous human diseases, and they constitute an estimated 30-40% of all drug targets. During the last two decades......, GPCR oligomerization has been extensively studied using methods like bioluminescence resonance energy transfer (BRET) and today, receptor-receptor interactions within the GPCR superfamily is a well-established phenomenon. Evidence of the impact of GPCR oligomerization on, e.g., ligand binding, receptor...

  12. Alternative Thieno[3,2-b][1]benzothiophene Isoindigo Polymers for Solar Cell Applications

    KAUST Repository

    Neophytou, Marios

    2018-03-05

    This work reports the synthesis, characterization, photophysical, and photovoltaic properties of five new thieno[3,2-b][1]benzothiophene isoindigo (TBTI)-containing low bandgap donor-acceptor conjugated polymers with a series of comonomers and different side chains. When TBTI is combined with different electron-rich moieties, even small structural variations can have significant impact on thin film morphology of the polymer:phenyl C70 butyric acid methyl ester (PCBM) blends. More importantly, high-resolution electron energy loss spectroscopy is used to investigate the phase-separated bulk heterojunction domains, which can be accurately and precisely resolved, enabling an enhanced correlation between polymer chemical structure, photovoltaic device performance, and morphology.

  13. Transverse charge and magnetization densities: Improved chiral predictions down to b=1 fms

    Energy Technology Data Exchange (ETDEWEB)

    Alarcon, Jose Manuel [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States); Hiller Blin, Astrid N. [Johannes Gutenberg Univ., Mainz (Germany); Vicente Vacas, Manuel J. [Spanish National Research Council (CSIC), Valencia (Spain). Univ. of Valencia (UV), Inst. de Fisica Corpuscular; Weiss, Christian [Thomas Jefferson National Accelerator Facility (TJNAF), Newport News, VA (United States)

    2018-03-01

    The transverse charge and magnetization densities provide insight into the nucleon’s inner structure. In the periphery, the isovector components are clearly dominant, and can be computed in a model-independent way by means of a combination of chiral effective field theory (cEFT) and dispersion analysis. With a novel N=D method, we incorporate the pion electromagnetic formfactor data into the cEFT calculation, thus taking into account the pion-rescattering effects and r-meson pole. As a consequence, we are able to reliably compute the densities down to distances b1 fm, therefore achieving a dramatic improvement of the results compared to traditional cEFT calculations, while remaining predictive and having controlled uncertainties.

  14. Aflatoxin B1Induced Systemic Toxicity in Poultry and Rescue Effects of Selenium and Zinc.

    Science.gov (United States)

    Mughal, Muhammad Jameel; Peng, Xi; Kamboh, Asghar Ali; Zhou, Yi; Fang, Jing

    2017-08-01

    Among many challenges, exposure to aflatoxins, particularly aflatoxin B 1 (AFB 1 ), is one of the major concerns in poultry industry. AFB 1 intoxication results in decreased meat/egg production, hepatotoxicity, nephrotoxicity, disturbance in gastrointestinal tract (GIT) and reproduction, immune suppression, and increased disease susceptibility. Selenium (Se) and zinc (Zn), in dietary supplementation, offer easy, cost-effective, and efficient ways to neutralize the toxic effect of AFB 1 . In the current review, we discussed the impact of AFB 1 on poultry industry, its biotransformation, and organ-specific noxious effects, along with the action mechanism of AFB 1 -induced toxicity. Moreover, we explained the biological and detoxifying roles of Se and Zn in avian species as well as the protection mechanism of these two trace elements. Ultimately, we discussed the use of Se and Zn supplementation against AFB 1 -induced toxicity in poultry birds.

  15. 1,3-Diphenyl-3,4-dihydrobenzo[b][1,6]naphthyridine

    Directory of Open Access Journals (Sweden)

    Werner Seebacher

    2010-05-01

    Full Text Available The title compound, C24H18N2, is the first structural example containing the 3,4-dihydrobenzo[b][1,6]naphthyridine fragment. It was synthesized from 2,4,6,8-tetraphenyl-3,7-diazabicyclo[3.3.1]nonan-9-one and was crystallized from a methanol–ethanol solution over two years as a racemate. The C=N double bond [1.2868 (15 Å] is bent significantly out of the plane of the aromatic bicyclic ring system [N—C—C—C = −157.63 (12°] and out of the plane of the phenyl ring bonded at the 1-position [N—C—C—C = 41.15 (16°].

  16. Excretion of Aflatoxin M1 in milk of goats fed diet contaminated by Aflatoxin B1

    Directory of Open Access Journals (Sweden)

    Gianni Battacone

    2010-01-01

    Full Text Available An experiment was carried out to study the excretion of aflatoxin M1(AFM1 in milk of three goats fed a single dose (0.8mg/head of pure aflatoxin B1 (AFB1. The values of AFM1 concentration excreted in milk was highly variable among goats, even if the pattern of excretion over time was very similar among the three animals. AFM1 was first detected at the milking performed 1h after the AFB1 administration. The highest values of AFM1 concentration in milk were reached 3 and 6h after the AFB1 intake. The trend of clearance of AFM1 in milk over time was expressed by a decreasing exponential equation. AFM1 concentration was below the EU maximum allowed level (50 ng/L in milk collected 36 h after the AFB1 administration.

  17. Differential inhibition of aflatoxin B1 oxidation by gestodene action on human liver microsomes.

    Science.gov (United States)

    Kim, B R; Oh, H S; Kim, D H

    1997-11-01

    Human cytochrome P450 (P450) 3A is known to be involved in the formation of both aflatoxin B1-exo-8,9-epoxide (exo-epoxidation) and aflatoxin Q1 (3 alpha-hydroxylation). Gestodene, a known inactivator of P450 3A4, inhibited the formation of AFB1 metabolites in a variety of ways depending on the incubation condition. Preincubation of gestodene with human liver microsomes prior to the addition of AFB1 inhibited both exo-epoxidation and 3 alpha-hydroxylation whereas simultaneous incubation of gestodene with AFB1 only inhibited 3 alpha-hydroxylation. These results suggest that two independent substrate binding sites exist in P450 3A4, and AFB1 binds to both of the binding sites. Gestodene selectively binds to one of the binding sites leading to the formation of AFQ1, whereas it does not affect the formation of exo-epoxide via the other binding site.

  18. Adsorptive stripping voltammetric study of vitamin B1 at multi-walled carbon nanotube paste electrode

    Directory of Open Access Journals (Sweden)

    Pradeep Kumar Brahman

    2016-11-01

    The reduction peak current varies linearly with the concentration of vitamin B1 over the range of 1.0 × 10−7–1.0 × 10−6 M. The limits of detection and quantification of the pure drug are 1.1 × 10−10 M and 2.0 × 10−9 M, with the correlation coefficient, r = 0.997 and the relative standard deviation, RSD = 1.2% (n = 5. Experimental results reveal that the MWCNTPE has advantages of small background current, high signal to background current ratio and good reproducibility.

  19. Direct Comparison of Physical Properties of Bacillus subtilis NCIB 3610 and B-1 Biofilms

    Science.gov (United States)

    Kesel, Sara; Grumbein, Stefan; Gümperlein, Ina; Tallawi, Marwa; Marel, Anna-Kristina

    2016-01-01

    Many bacteria form surface-attached communities known as biofilms. Due to the extreme resistance of these bacterial biofilms to antibiotics and mechanical stresses, biofilms are of growing interest not only in microbiology but also in medicine and industry. Previous studies have determined the extracellular polymeric substances present in the matrix of biofilms formed by Bacillus subtilis NCIB 3610. However, studies on the physical properties of biofilms formed by this strain are just emerging. In particular, quantitative data on the contributions of biofilm matrix biopolymers to these physical properties are lacking. Here, we quantitatively investigated three physical properties of B. subtilis NCIB 3610 biofilms: the surface roughness and stiffness and the bulk viscoelasticity of these biofilms. We show how specific biomolecules constituting the biofilm matrix formed by this strain contribute to those biofilm properties. In particular, we demonstrate that the surface roughness and surface elasticity of 1-day-old NCIB 3610 biofilms are strongly affected by the surface layer protein BslA. For a second strain, B. subtilis B-1, which forms biofilms containing mainly γ-polyglutamate, we found significantly different physical biofilm properties that are also differently affected by the commonly used antibacterial agent ethanol. We show that B-1 biofilms are protected from ethanol-induced changes in the biofilm's stiffness and that this protective effect can be transferred to NCIB 3610 biofilms by the sole addition of γ-polyglutamate to growing NCIB 3610 biofilms. Together, our results demonstrate the importance of specific biofilm matrix components for the distinct physical properties of B. subtilis biofilms. PMID:26873313

  20. Identification of homozygous deletions at chromosome 16q23 in aflatoxin B1 exposed hepatocellular carcinoma.

    Science.gov (United States)

    Yakicier, M C; Legoix, P; Vaury, C; Gressin, L; Tubacher, E; Capron, F; Bayer, J; Degott, C; Balabaud, C; Zucman-Rossi, J

    2001-08-23

    Loss of heterozygosity (LOH) represents the most frequent genetic alteration observed in hepatocellular carcinoma (HCC). Chromosome 16q is of particular interest as it exhibits LOH in 29% of HCC tumors and is frequently lost in breast, prostate, ovarian and gastric carcinomas. We genotyped 157 HCC tumors for 17 microsatellite markers distributed on chromosome 16q and determined a common region of LOH localized between the markers D16S518 and D16S504. By refining the boundaries of two interstitial LOH and two homozygous deletions, the critical region was delimited to 180 kb between D16S3096 and D16S3029. This region is located in intron 8 of the WWOX/FOR gene, but a search for mutations in all coding exons of this gene in 27 HCC tumors and cell lines did not reveal any tumor somatic alterations. Furthermore, by RT-PCR, no abnormal transcripts of this WWOX/FOR gene was detected in nine HCC cell lines. Finally, analysis of the p53 gene mutations with the clinical parameters of all tumors revealed that the two homozygous deletions have occurred in tumors presenting a R249S mutation. Our data revealed a relationship between chromosome 16q homozygous deletions and R249S p53 mutations in tumors where the patient had been exposed to aflatoxin B1 (P=0.002). These results are consistent with a role of aflatoxin B1 in the instability of chromosome 16q at the fragile site FRA16D. However, the nature of the specific gene that is altered during hepatocarcinogenesis remains to be elucidated.