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Sample records for stable radiometal antibody

  1. Extracorporeal adsorption therapy: A Method to improve targeted radiation delivered by radiometal-labeled monoclonal antibodies

    International Nuclear Information System (INIS)

    Nemecek, Eneida R.; Green, Damian J.; Fisher, Darrell R.; Pagal, John M.; Lin, Yukang; Gopal, A. K.; Durack, Lawrence D.; Rajendran, Joseph G.; Wilbur, D. S.; Nilsson, Rune; Sandberg, Bengt; Press, Oliver W.

    2008-01-01

    Many investigators have demonstrated the ability to treat hematologic malignancies with radiolabeled monoclonal antibodies targeting hematopoietic antigens such as anti-CD20 and anti-CD45. [1-5] Although the remission rates achieved with radioimmunotherapy (RIT) are relatively high, many patients subsequently relapse presumably due to suboptimal delivery of enough radiation to eradicate the malignancy. The dose-response of leukemia and lymphoma to radiation has been proven. Substantial amounts of radiation can be delivered by RIT if followed by hematopoietic cell transplantation to rescue the bone marrow from myeloablation.[ref] However, the maximum dose of RIT that can be used is still limited by toxicity to normal tissues affected by nonspecific delivery of radiation. Efforts to improve RIT focus on improving the therapeutic ratios of radiation in target versus non-target tissues by removing the fraction of radioisotope that fails to bind to target tissues and circulates freely in the bloodstream perfusing non-target tissues. Our group and others have explored several alternatives for removal of unbound circulating antibody. [refs] One such method, extracorporeal adsorption therapy (ECAT) consists of removing unbound antibody by a method similar to plasmapheresis after critical circulation time and distribution of antibody into target tissues have been achieved. Preclinical studies of ECAT in murine xenograft models demonstrated significant improvement in therapeutic ratios of radioactivity. Chen and colleagues demonstrated that a 2-hour ECAT procedure could remove 40 to 70% of the radioactivity from liver, lung and spleen. [ref] Although isotope concentration in the tumor was initially unaffected, a 50% decrease was noted approximately 36 hours after the procedure. This approach was also evaluated in a limited phase I pilot study of patients with refractory B-cell lymphoma. [ref] After radiographic confirmation of tumor localization of a test dose of anti-CD20

  2. PHOCUS radiometer

    Directory of Open Access Journals (Sweden)

    O. Nyström

    2012-06-01

    Full Text Available PHOCUS – Particles, Hydrogen and Oxygen Chemistry in the Upper Summer Mesosphere is a Swedish sounding rocket experiment, launched in July 2011, with the main goal of investigating the upper atmosphere in the altitude range 50–110 km. This paper describes the SondRad instrument in the PHOCUS payload, a radiometer comprising two frequency channels (183 GHz and 557 GHz aimed at exploring the water vapour concentration distribution in connection with the appearance of noctilucent (night shining clouds. The design of the radiometer system has been done in a collaboration between Omnisys Instruments AB and the Group for Advanced Receiver Development (GARD at Chalmers University of Technology where Omnisys was responsible for the overall design, implementation, and verification of the radiometers and backend, whereas GARD was responsible for the radiometer optics and calibration systems.

    The SondRad instrument covers the water absorption lines at 183 GHz and 557 GHz. The 183 GHz channel is a side-looking radiometer, while the 557 GHz radiometer is placed along the rocket axis looking in the forward direction. Both channels employ sub-harmonically pumped Schottky mixers and Fast Fourier Transform Spectrometers (FFTS backends with 67 kHz resolution.

    The radiometers include novel calibration systems specifically adjusted for use with each frequency channel. The 183 GHz channel employs a continuous wave CW pilot signal calibrating the entire receiving chain, while the intermediate frequency chain (the IF-chain of the 557 GHz channel is calibrated by injecting a signal from a reference noise source through a directional coupler.

    The instrument collected complete spectra for both the 183 GHz and the 557 GHz with 300 Hz data rate for the 183 GHz channel and 10 Hz data rate for the 557 GHz channel for about 60 s reaching the apogee of the flight trajectory and 100 s after that. With lossless data compression using variable

  3. Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particles.

    Directory of Open Access Journals (Sweden)

    Ran Taube

    Full Text Available BACKGROUND: Isolation of human antibodies using current display technologies can be limited by constraints on protein expression, folding and post-translational modifications. Here we describe a discovery platform that utilizes self-inactivating (SIN lentiviral vectors for the surface display of high-affinity single-chain variable region (scFv antibody fragments on human cells and lentivirus particles. METHODOLOGY/PRINCIPAL FINDINGS: Bivalent scFvFc human antibodies were fused in frame with different transmembrane (TM anchoring moieties to allow efficient high-level expression on human cells and the optimal TM was identified. The addition of an eight amino acid HIV-1 gp41 envelope incorporation motif further increased scFvFc expression on human cells and incorporation into lentiviral particles. Both antibody-displaying human cells and virus particles bound antigen specifically. Sulfation of CDR tyrosine residues, a property recently shown to broaden antibody binding affinity and antigen recognition was also demonstrated. High level scFvFc expression and stable integration was achieved in human cells following transduction with IRES containing bicistronic SIN lentivectors encoding ZsGreen when scFvFc fusion proteins were expressed from the first cassette. Up to 10(6-fold enrichment of antibody expressing cells was achieved with one round of antigen coupled magnetic bead pre-selection followed by FACS sorting. Finally, the scFvFc displaying human cells could be used directly in functional biological screens with remarkable sensitivity. CONCLUSIONS/SIGNIFICANCE: This antibody display platform will complement existing technologies by virtue of providing properties unique to lentiviruses and antibody expression in human cells, which, in turn, may aid the discovery of novel therapeutic human mAbs.

  4. Biological Characterization of a Stable Effector Functionless (SEFL) Monoclonal Antibody Scaffold in Vitro*

    Science.gov (United States)

    Liu, Ling; Jacobsen, Frederick W.; Everds, Nancy; Zhuang, Yao; Yu, Yan Bin; Li, Nianyu; Clark, Darcey; Nguyen, Mai Phuong; Fort, Madeline; Narayanan, Padma; Kim, Kei; Stevenson, Riki; Narhi, Linda; Gunasekaran, Kannan; Bussiere, Jeanine L.

    2017-01-01

    The stable effector functionLess (SEFL) antibody was designed as an IgG1 antibody with a constant region that lacks the ability to interact with Fcγ receptors. The engineering and stability and pharmacokinetic assessments of the SEFL scaffold is described in the accompanying article (Jacobsen, F. W., Stevenson, R., Li, C., Salimi-Moosavi, H., Liu, L., Wen, J., Luo, Q., Daris, K., Buck, L., Miller, S., Ho, S-Y., Wang, W., Chen, Q., Walker, K., Wypych, J., Narhi, L., and Gunasekaran, K. (2017) J. Biol. Chem. 292). The biological properties of these SEFL antibodies were assessed in a variety of human and cynomolgus monkey in vitro assays. Binding of parent molecules and their SEFL variants to human and cynomolgus monkey FcγRs were evaluated using flow cytometry-based binding assays. The SEFL variants tested showed decreased binding affinity to human and cynomolgus FcγRs compared with the wild-type IgG1 antibody. In addition, SEFL variants demonstrated no antibody-dependent cell-mediated cytotoxicity in vitro against Daudi cells with cynomolgus monkey peripheral blood mononuclear cells, and had minimal complement-dependent cytotoxicity activity similar to that of the negative control IgG2 in a CD20+ human Raji lymphoma cell line. SEFL mutations eliminated off-target antibody-dependent monocyte phagocytosis of cynomolgus monkey platelets, and cynomolgus platelet activation in vitro. These experiments demonstrate that the SEFL modifications successfully eliminated Fc-associated effector binding and functions. PMID:27994063

  5. Comparative analysis of monoclonal antibody N-glycosylation using stable isotope labelling and UPLC-fluorescence-MS.

    Science.gov (United States)

    Millán Martín, Silvia; Delporte, Cédric; Farrell, Amy; Navas Iglesias, Natalia; McLoughlin, Niaobh; Bones, Jonathan

    2015-03-07

    A twoplex method using (12)C6 and (13)C6 stable isotope analogues (Δmass = 6 Da) of 2-aminobenzoic acid (2-AA) is described for quantitative analysis of N-glycans present on monoclonal antibodies and other glycoproteins using ultra performance liquid chromatography with sequential fluorescence and accurate mass tandem quadrupole time of flight (QToF) mass spectrometric detection.

  6. Development of a stable radioiodinating reagent to label monoclonal antibodies for radiotherapy of cancer

    International Nuclear Information System (INIS)

    Wilbur, D.S.; Hadley, S.W.; Hylarides, M.D.; Abrams, P.G.; Beaumier, P.A.; Morgan, A.C.; Reno, J.M.; Fritzberg, A.R.

    1989-01-01

    A method of radioiodinating monoclonal antibodies such that the labeled antibodies do not undergo in vivo deiodination has been studied. The method utilizes conjugation of succinimidyl para-iodobenzoate to the antibody. The iodobenzoate was radiolabeled by using an organometallic intermediate to facilitate the reaction. Thus, succinimidyl para-tri-n-butylstannylbenzoate was radiolabeled in 60-90% radiochemical yield and subsequently conjugated to the antibody in 80-90% yield. Animal biodistribution studies were carried out with two separate anti-melanoma antibodies (9.2.27 and NR-M1-05) labeled by this method, and examined in nude mice bearing human melanoma tumor xenografts. Very large differences in the localization of radioactivity were observed in the thyroids and stomachs of mice when the iodobenzoyl-labeled antibodies were compared with the same antibodies labeled using the chloramine-T method of radioiodination. Few other significant differences in the tissue distribution of the radioiodinated antibodies were seen

  7. Monomeric CH3: A Small, Stable Antibody Domain with Therapeutic Promise | Poster

    Science.gov (United States)

    By Ashley DeVine, Staff Writer Antibody domains are emerging as promising biopharmaceuticals because of their relatively small size compared to full-sized antibodies, which are too large to effectively penetrate tumors and bind to sterically restricted therapeutic targets. In an article published in The Journal of Biological Chemistry, Tianlei Ying, Ph.D., Dimiter Dimitrov,

  8. Monitored background radiometer

    International Nuclear Information System (INIS)

    Ruel, C.

    1988-01-01

    A monitored background radiometer is described comprising: a thermally conductive housing; low conductivity support means mounted on the housing; a sensing plate mounted on the low conductivity support means and spaced from the housing so as to be thermally insulated from the housing and having an outwardly facing first surface; the sensing plate being disposed relative to the housing to receive direct electromagnetic radiation from sources exterior to the radiometer upon the first surface only; means for controllably heating the sensing plate; first temperature sensitive means to measure the temperature of the housing; and second temperature sensitive means to measure the temperature of the sensing plate, so that the heat flux at the sensing plate may be determined from the temperatures of the housing and sensing plate after calibration of the radiometer by measuring the temperatures of the housing and sensing plate while controllably heating the sensing plate

  9. Elaboration of Stable and Antibody Functionalized Positively Charged Colloids by Polyelectrolyte Complexation between Chitosan and Hyaluronic Acid

    Directory of Open Access Journals (Sweden)

    Ramona C. Polexe

    2013-07-01

    Full Text Available In this study, we describe the elaboration of multifunctional positively charged polyelectrolyte complex (PEC nanoparticles, designed to be stable at physiological salt concentration and pH, for effective targeted delivery. These nanoparticles were obtained by charge neutralization between chitosan (CS as polycation and hyaluronic acid (HA as polyanion. We showed that the course of the complexation process and the physico-chemical properties of the resulting colloids were impacted by (i internal parameters such as the Degree of Acetylation (DA, i.e., the molar ration of acetyl glucosamine residues and molar mass of CS, the HA molar mass and (ii external parameters like the charge mixing ratio and the polymer concentrations. As a result, nonstoichiometric colloidal PECs were obtained in water or PBS (pH 7.4 and remained stable over one month. The polymer interactions were characterized by thermal analysis (DSC and TGA and the morphology was studied by scanning electron microscopy. A model antibody, anti-ovalbumine (OVA immunoglobulin A (IgA was sorbed on the particle surface in water and PBS quantitatively in 4 h. The CS-HA/IgA nanoparticles average size was between 425–665 nm with a positive zeta potential. These results pointed out that CS-HA can be effective carriers for use in targeted drug delivery.

  10. Aquarius L-Band Radiometers Calibration Using Cold Sky Observations

    Science.gov (United States)

    Dinnat, Emmanuel P.; Le Vine, David M.; Piepmeier, Jeffrey R.; Brown, Shannon T.; Hong, Liang

    2015-01-01

    An important element in the calibration plan for the Aquarius radiometers is to look at the cold sky. This involves rotating the satellite 180 degrees from its nominal Earth viewing configuration to point the main beams at the celestial sky. At L-band, the cold sky provides a stable, well-characterized scene to be used as a calibration reference. This paper describes the cold sky calibration for Aquarius and how it is used as part of the absolute calibration. Cold sky observations helped establish the radiometer bias, by correcting for an error in the spillover lobe of the antenna pattern, and monitor the long-term radiometer drift.

  11. BETA digital beta radiometer

    International Nuclear Information System (INIS)

    Borovikov, N.V.; Kosinov, G.A.; Fedorov, Yu.N.

    1989-01-01

    Portable transportable digital beta radiometer providing for measuring beta-decay radionuclide specific activity in the range from 5x10 -9 up to 10 -6 Cu/kg (Cu/l) with error of ±25% is designed and introduced into commercial production for determination of volume and specific water and food radioactivity. The device specifications are given. Experience in the BETA radiometer application under conditions of the Chernobyl' NPP 30-km zone has shown that it is convenient for measuring specific activity of the order of 10 -8 Cu/kg, and application of a set of different beta detectors gives an opportunity to use it for surface contamination measurement in wide range of the measured value

  12. Millimeter radiometer system technology

    Science.gov (United States)

    Wilson, W. J.; Swanson, P. N.

    1989-07-01

    JPL has had a large amount of experience with spaceborne microwave/millimeter wave radiometers for remote sensing. All of the instruments use filled aperture antenna systems from 5 cm diameter for the microwave Sounder Units (MSU), 16 m for the microwave limb sounder (MLS) to 20 m for the large deployable reflector (LDR). The advantages of filled aperture antenna systems are presented. The requirements of the 10 m Geoplat antenna system, 10 m multified antenna, and the MLS are briefly discussed.

  13. Hurricane Imaging Radiometer

    Science.gov (United States)

    Cecil, Daniel J.; Biswas, Sayak K.; James, Mark W.; Roberts, J. Brent; Jones, W. Linwood; Johnson, James; Farrar, Spencer; Sahawneh, Saleem; Ruf, Christopher S.; Morris, Mary; hide

    2014-01-01

    The Hurricane Imaging Radiometer (HIRAD) is a synthetic thinned array passive microwave radiometer designed to allow retrieval of surface wind speed in hurricanes, up through category five intensity. The retrieval technology follows the Stepped Frequency Microwave Radiometer (SFMR), which measures surface wind speed in hurricanes along a narrow strip beneath the aircraft. HIRAD maps wind speeds in a swath below the aircraft, about 50-60 km wide when flown in the lower stratosphere. HIRAD has flown in the NASA Genesis and Rapid Intensification Processes (GRIP) experiment in 2010 on a WB-57 aircraft, and on a Global Hawk unmanned aircraft system (UAS) in 2012 and 2013 as part of NASA's Hurricane and Severe Storms Sentinel (HS3) program. The GRIP program included flights over Hurricanes Earl and Karl (2010). The 2012 HS3 deployment did not include any hurricane flights for the UAS carrying HIRAD. The 2013 HS3 flights included one flight over the predecessor to TS Gabrielle, and one flight over Hurricane Ingrid. This presentation will describe the HIRAD instrument, its results from the 2010 and 2013 flights, and potential future developments.

  14. Calibration of Correlation Radiometers Using Pseudo-Random Noise Signals

    Directory of Open Access Journals (Sweden)

    Sebastián Pantoja

    2009-08-01

    Full Text Available The calibration of correlation radiometers, and particularly aperture synthesis interferometric radiometers, is a critical issue to ensure their performance. Current calibration techniques are based on the measurement of the cross-correlation of receivers’ outputs when injecting noise from a common noise source requiring a very stable distribution network. For large interferometric radiometers this centralized noise injection approach is very complex from the point of view of mass, volume and phase/amplitude equalization. Distributed noise injection techniques have been proposed as a feasible alternative, but are unable to correct for the so-called “baseline errors” associated with the particular pair of receivers forming the baseline. In this work it is proposed the use of centralized Pseudo-Random Noise (PRN signals to calibrate correlation radiometers. PRNs are sequences of symbols with a long repetition period that have a flat spectrum over a bandwidth which is determined by the symbol rate. Since their spectrum resembles that of thermal noise, they can be used to calibrate correlation radiometers. At the same time, since these sequences are deterministic, new calibration schemes can be envisaged, such as the correlation of each receiver’s output with a baseband local replica of the PRN sequence, as well as new distribution schemes of calibration signals. This work analyzes the general requirements and performance of using PRN sequences for the calibration of microwave correlation radiometers, and particularizes the study to a potential implementation in a large aperture synthesis radiometer using an optical distribution network.

  15. A new approach for generating bispecific antibodies based on a common light chain format and the stable architecture of human immunoglobulin G1.

    Science.gov (United States)

    De Nardis, Camilla; Hendriks, Linda J A; Poirier, Emilie; Arvinte, Tudor; Gros, Piet; Bakker, Alexander B H; de Kruif, John

    2017-09-01

    Bispecific antibodies combine two different antigen-binding sites in a single molecule, enabling more specific targeting, novel mechanisms of action, and higher clinical efficacies. Although they have the potential to outperform conventional monoclonal antibodies, many bispecific antibodies have issues regarding production, stability, and pharmacokinetic properties. Here, we describe a new approach for generating bispecific antibodies using a common light chain format and exploiting the stable architecture of human immunoglobulin G 1 We used iterative experimental validation and computational modeling to identify multiple Fc variant pairs that drive efficient heterodimerization of the antibody heavy chains. Accelerated stability studies enabled selection of one Fc variant pair dubbed "DEKK" consisting of substitutions L351D and L368E in one heavy chain combined with L351K and T366K in the other. Solving the crystal structure of the DEKK Fc region at a resolution of 2.3 Å enabled detailed analysis of the interactions inducing CH3 interface heterodimerization. Local shifts in the IgG backbone accommodate the introduction of lysine side chains that form stabilizing salt-bridge interactions with substituted and native residues in the opposite chain. Overall, the CH3 domain adapted to these shifts at the interface, yielding a stable Fc conformation very similar to that in wild-type IgG. Using the DEKK format, we generated the bispecific antibody MCLA-128, targeting human EGF receptors 2 and 3. MCLA-128 could be readily produced and purified at industrial scale with a standard mammalian cell culture platform and a routine purification protocol. Long-term accelerated stability assays confirmed that MCLA-128 is highly stable and has excellent biophysical characteristics. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  16. Monolithic microwave integrated circuit water vapor radiometer

    Science.gov (United States)

    Sukamto, L. M.; Cooley, T. W.; Janssen, M. A.; Parks, G. S.

    1991-01-01

    A proof of concept Monolithic Microwave Integrated Circuit (MMIC) Water Vapor Radiometer (WVR) is under development at the Jet Propulsion Laboratory (JPL). WVR's are used to remotely sense water vapor and cloud liquid water in the atmosphere and are valuable for meteorological applications as well as for determination of signal path delays due to water vapor in the atmosphere. The high cost and large size of existing WVR instruments motivate the development of miniature MMIC WVR's, which have great potential for low cost mass production. The miniaturization of WVR components allows large scale deployment of WVR's for Earth environment and meteorological applications. Small WVR's can also result in improved thermal stability, resulting in improved calibration stability. Described here is the design and fabrication of a 31.4 GHz MMIC radiometer as one channel of a thermally stable WVR as a means of assessing MMIC technology feasibility.

  17. Monitored background radiometer

    International Nuclear Information System (INIS)

    Ruel, C.

    1988-01-01

    This radiometer accurately measures IR and solar spectrum radiation in a vacuum, and accounts for radiation loss from its sensing plate by measuring the housing temperature. Calibration is performed by measuring the temperature of the sensing plate and housing while power to a heater attached to the sensing plate is varied. The square of the difference between the measured power dissipation of the heater and the heat absorbed by the sensing plate as determined from the heat balance equation of the sensing plate is minimized to obtain calibration factors for the heat balance equation

  18. A Multifrequency Radiometer System

    DEFF Research Database (Denmark)

    Skou, Niels

    1977-01-01

    A radiometer system having four channels: 5 GHz, l7 GHz, 34 GHz, all vertical polarization, and a 34 GHz sky horn, will be described. The system which is designed for collecting glaciological and oceanographic data is intended for airborne use and imaging is achieved by means of a multifrequency...... conically scanning antenna. Implementation of the noise-injection technique ensures the high absolute accuracy needed for oceanographic purposes. The collected data can be preprocessed in a microcomputer system and displayed in real time. Simultaneously, the data are recorded digitally on tape for more...

  19. Glutamine synthetase gene knockout-human embryonic kidney 293E cells for stable production of monoclonal antibodies.

    Science.gov (United States)

    Yu, Da Young; Lee, Sang Yoon; Lee, Gyun Min

    2018-05-01

    Previously, it was inferred that a high glutamine synthetase (GS) activity in human embryonic kidney (HEK) 293E cells results in elevated resistance to methionine sulfoximine (MSX) and consequently hampers GS-mediated gene amplification and selection by MSX. To overcome this MSX resistance in HEK293E cells, a GS-knockout HEK293E cell line was generated using the CRISPR/Cas9 system to target the endogenous human GS gene. The GS-knockout in the HEK293E cell line (RK8) was confirmed by Western blot analysis of GS and by observation of glutamine-dependent growth. Unlike the wild type HEK293E cells, the RK8 cells were successfully used as host cells to generate a recombinant HEK293E cell line (rHEK293E) producing a monoclonal antibody (mAb). When the RK8 cells were transfected with the GS expression vector containing the mAb gene, rHEK293E cells producing the mAb could be selected in the absence as well as in the presence of MSX. The gene copies and mRNA expression levels of the mAb in rHEK293E cells were also quantified using qRT-PCR. Taken together, the GS-knockout HEK293E cell line can be used as host cells to generate stable rHEK293E cells producing a mAb through GS-mediated gene selection in the absence as well as in the presence of MSX. © 2018 Wiley Periodicals, Inc.

  20. Challenges in application of Active Cold Loads for microwave radiometer calibration

    DEFF Research Database (Denmark)

    Søbjærg, Sten Schmidl; Balling, Jan E.; Skou, Niels

    2012-01-01

    Two Active Cold Loads (ACLs) for microwave radiometer calibration, operating at X-band, are evaluated with respect to important stability parameters. Using a stable radiometer system as test bed, absolute levels of 77 K and 55 K are found. This paper identifies and summarizes potential challenges...

  1. Microfluidic Radiometal Labeling Systems for Biomolecules

    Energy Technology Data Exchange (ETDEWEB)

    Reichert, D E; Kenis, P J. A.

    2011-12-29

    In a typical labeling procedure with radiometals, such as Cu-64 and Ga-68; a very large (~ 100-fold) excess of the non-radioactive reactant (precursor) is used to promote rapid and efficient incorporation of the radioisotope into the PET imaging agent. In order to achieve high specific activities, careful control of reaction conditions and extensive chromatographic purifications are required in order to separate the labeled compounds from the cold precursors. Here we propose a microfluidic approach to overcome these problems, and achieve high specific activities in a more convenient, semi-automated fashion and faster time frame. Microfluidic reactors, consisting of a network of micron-sized channels (typical dimensions in the range 10 - 300¼m), filters, separation columns, electrodes and reaction loops/chambers etched onto a solid substrate, are now emerging as an extremely useful technology for the intensification and miniaturization of chemical processes. The ability to manipulate, process and analyze reagent concentrations and reaction interfaces in both space and time within the channel network of a microreactor provides the fine level of reaction control that is desirable in PET radiochemistry practice. These factors can bring radiometal labeling, specifically the preparation of radio-labeled biomolecules such as antibodies, much closer to their theoretical maximum specific activities.

  2. Analyzing Non Stationary Processes in Radiometers

    Science.gov (United States)

    Racette, Paul

    2010-01-01

    The lack of well-developed techniques for modeling changing statistical moments in our observations has stymied the application of stochastic process theory for many scientific and engineering applications. Non linear effects of the observation methodology is one of the most perplexing aspects to modeling non stationary processes. This perplexing problem was encountered when modeling the effect of non stationary receiver fluctuations on the performance of radiometer calibration architectures. Existing modeling approaches were found not applicable; particularly problematic is modeling processes across scales over which they begin to exhibit non stationary behavior within the time interval of the calibration algorithm. Alternatively, the radiometer output is modeled as samples from a sequence random variables; the random variables are treated using a conditional probability distribution function conditioned on the use of the variable in the calibration algorithm. This approach of treating a process as a sequence of random variables with non stationary stochastic moments produce sensible predictions of temporal effects of calibration algorithms. To test these model predictions, an experiment using the Millimeter wave Imaging Radiometer (MIR) was conducted. The MIR with its two black body calibration references was configured in a laboratory setting to observe a third ultra-stable reference (CryoTarget). The MIR was programmed to sequentially sample each of the three references in approximately a 1 second cycle. Data were collected over a six-hour interval. The sequence of reference measurements form an ensemble sample set comprised of a series of three reference measurements. Two references are required to estimate the receiver response. A third reference is used to estimate the uncertainty in the estimate. Typically, calibration algorithms are designed to suppress the non stationary effects of receiver fluctuations. By treating the data sequence as an ensemble

  3. Double-polarizating scanning radiometer

    International Nuclear Information System (INIS)

    Mishev, D.N.; Nazyrski, T.G.

    1986-01-01

    The double-polarizating single-channel scanning radiometer comprises the following serial connected parts: a scanning double-polarizating aerial, a block for polarization separation, a radiometer receiver, an analog-to-digit converter and an information flow forming block. The low frequency input of the radiometer receiver is connected with a control block, which is also connected with a first bus of a microprocessor, the second bus of which is connected with the A-D converter. The control input of the scanning double-polarizating aerial is connected with the first microprocessor bus. The control inputs of the block for polarization separation are linked by an electronic switch with the output of the forming block, the input of which is connected to the first input of the control block. The control inputs of the block for polarization separation are connected with the second and the third input of the information flow forming block. 2 cls

  4. Radiometer Testbed Development for SWOT

    Science.gov (United States)

    Kangaslahti, Pekka; Brown, Shannon; Gaier, Todd; Dawson, Douglas; Harding, Dennis; Fu, Lee-Lueng; Esteban-Fernandez, Daniel

    2010-01-01

    Conventional altimeters include nadir looking colocated 18-37 GHz microwave radiometer to measure wet tropospheric path delay. These have reduced accuracy in coastal zone (within 50 km from land) and do not provide wet path delay over land. The addition of high frequency channels to Jason-class radiometer will improve retrievals in coastal regions and enable retrievals over land. High-frequency window channels, 90, 130 and 166 GHz are optimum for improving performance in coastal region and channels on 183 GHz water vapor line are ideal for over-land retrievals.

  5. Antithyroglobulin antibody

    Science.gov (United States)

    Thyroglobulin antibody; Thyroiditis - thyroglobulin antibody; Hypothyroidism - thyroglobulin antibody; Thyroiditis - thyroglobulin antibody; Graves disease - thyroglobulin antibody; Underactive thyroid - thyroglobulin antibody

  6. Microwave Radiometer Systems, Design and Analysis

    DEFF Research Database (Denmark)

    Skou, Niels; Vine, David Le

    Two important microwave remote sensors are the radar and the radiometer. There have been a number of books written on various aspects of radar, but there have been only a few written on microwave radiometers, especially on subjects of how to design and build radiometer systems. This book, which...

  7. Microwave Radiometer Linearity Measured by Simple Means

    DEFF Research Database (Denmark)

    Skou, Niels

    2002-01-01

    Modern spaceborne radiometer systems feature an almost perfect on-board calibration, hence the primary calibration task to be carried out before launch is a check of radiometer linearity. This paper describes two ways of measuring linearity of microwave radiometers only requiring relatively simple...

  8. Nature of the bifunctional chelating agent used for radioimmunotherapy with yttrium-88 monoclonal antibodies: critical factors in determining in vivo survival and organ toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Kozak, R.W.; Raubitschek, A.; Mirzadeh, S.; Brechbiel, M.W.; Junghaus, R.; Gansow, O.A.; Waldmann, T.A. (Center for Biologics Evaluation and Research, FDA, Bethesda, MD (USA))

    1989-05-15

    One factor that is critical to the potential effectiveness of radioimmunotherapy is the design of radiometal-chelated antibodies that will be stable in vivo. Stability in vivo depends on the condition that both the chelate linkage and radiolabeling procedures not alter antibody specificity and biodistribution. In addition, synthesis and selection of the chelating agent is critical for each radiometal in order to prevent inappropriate release of the radiometal in vivo. In the present study, we compare the in vivo stability of seven radioimmunoconjugates that use different polyaminocarboxylate chelating agents to complex yttrium-88 to the mouse anti-human interleukin-2 receptor monoclonal antibody, anti-Tac. Chelate linkage and radiolabeling procedures did not alter the immunospecificity of anti-Tac. In order to assess whether yttrium was inappropriately released from the chelate-coupled antibody in vivo, iodine-131-labeled and yttrium-88 chelate-coupled antibodies were simultaneously administered to the same animals to correlate the decline in yttrium and radioiodinated antibody activity. The four stable yttrium-88 chelate-coupled antibodies studied displayed similar iodine-131 and yttrium-88 activity, indicating minimal elution of yttrium-88 from the complex. In contrast, the unstable yttrium-88 chelate-coupled antibodies had serum yttrium-88 activities that declined much more rapidly than their iodine-131 activities, suggesting loss of the radiolabel yttrium-88 from the chelate. Furthermore, high rates of yttrium-88 elution correlated with deposition in bone. Four chelating agents emerged as promising immunotherapeutic reagents: isothiocyanate benzyl DTPA and its derivatives 1B3M, MX, and 1M3B.

  9. A Scanning Microwave Radar and Radiometer

    DEFF Research Database (Denmark)

    Skou, Niels

    1995-01-01

    The Scanning Microwave Radar and Radiometer (SMRR) is a line scanner featuring a combined radar and radiometer system operating around 35 and 94 GHz. The layout of the SMRR is shown. The 2 offset antenna parabolas scan in synchronism, the receiver antenna has the highest gain in order to ensure...

  10. Source analysis of spaceborne microwave radiometer interference over land

    Science.gov (United States)

    Guan, Li; Zhang, Sibo

    2016-03-01

    Satellite microwave thermal emissions mixed with signals from active sensors are referred to as radiofrequency interference (RFI). Based on Advanced Microwave Scanning Radiometer-Earth Observing System (AMSR-E) observations from June 1 to 16, 2011, RFI over Europe was identified and analyzed using the modified principal component analysis algorithm in this paper. The X band AMSR-E measurements in England and Italy are mostly affected by the stable, persistent, active microwave transmitters on the surface, while the RFI source of other European countries is the interference of the reflected geostationary TV satellite downlink signals to the measurements of spaceborne microwave radiometers. The locations and intensities of the RFI induced by the geostationary TV and communication satellites changed with time within the observed period. The observations of spaceborne microwave radiometers in ascending portions of orbits are usually interfered with over European land, while no RFI was detected in descending passes. The RFI locations and intensities from the reflection of downlink radiation are highly dependent upon the relative geometry between the geostationary satellite and the measuring passive sensor. Only these fields of view of a spaceborne instrument whose scan azimuths are close to the azimuth relative to the geostationary satellite are likely to be affected by RFI.

  11. CAMEX-3 POLARIMETRIC SCANNING RADIOMETER (PSR) V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The Polarimetric Scanning Radiometer (PSR) is a versatile airborne microwave imaging radiometer developed by the Georgia Institute of Technology and the NOAA...

  12. Microwave Radiometry and Radiometers for Ocean Applications

    DEFF Research Database (Denmark)

    Skou, Niels

    2008-01-01

    The microwave radiometer system measures, within its bandwidth, the naturally emitted radiation – the brightness temperature – of substances within its antenna’s field of view. Thus a radiometer is really a sensitive and calibrated microwave receiver. The radiometer can be a basic total power....../antenna size, and the problem: scanning antenna/space- craft stability. In many cases good compromises have been reached, as evident recalling the many successful missions throughout the recent 30 years. But in some cases the situation calls for special solutions, like the push-broom system or the synthetic...

  13. GRIP HURRICANE IMAGING RADIOMETER (HIRAD) V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The GRIP Hurricane Imaging Radiometer (HIRAD) V1 dataset contains measurements of brightness temperature taken at 4, 5, 6 and 6.6 GHz, as well as MERRA 2 m wind...

  14. Microwave Radiometry and Radiometers for Ocean Applications

    DEFF Research Database (Denmark)

    Skou, Niels

    2008-01-01

    aperture radiometer technique, both yielding imaging capability without scanning. Typical applications of microwave radiometry concerning oceans are: sea salinity, sea surface temperature, wind speed and direction, sea ice detection and classification. However, in an attempt to measure properties...

  15. Accurate frequency measurements on gyrotrons using a ''gyro-radiometer''

    International Nuclear Information System (INIS)

    Rebuffi, L.

    1986-08-01

    Using an heterodyne system, called ''Gyro-radiometer'', accurated frequency measurements have been carried out on VARIAN 60 GHz gyrotrons. Changing the principal tuning parameters of a gyrotron, we have detected frequency variations up to 100 MHz, ∼ 40 MHz frequency jumps and smaller jumps (∼ 10 MHz) when mismatches in the transmission line were present. FWHM bandwidth of 300 KHz, parasitic frequencies and frequency drift during 100 msec pulses have also been observed. An efficient method to find a stable-, high power-, long pulse-working point of a gyrotron loaded by a transmission line, has been derived. In general, for any power value it is possible to find stable working conditions tuning the principal parameters of the tube in correspondance of a maximum of the emitted frequency

  16. Novel Cyclotron-Based Radiometal Production

    International Nuclear Information System (INIS)

    DeGrado, Timothy R.

    2013-01-01

    Accomplishments: (1) Construction of prototype solution target for radiometal production; (2) Testing of prototype target for production of following isotopes: a. Zr-89. Investigation of Zr-89 production from Y-89 nitrate solution. i. Defined problems of gas evolution and salt precipitation. ii. Solved problem of precipitation by addition of nitric acid. iii. Solved gas evolution problem with addition of backpressure regulator and constant degassing of target during irradiations. iv. Investigated effects of Y-89 nitrate concentration and beam current. v. Published abstracts at SNM and ISRS meetings; (3) Design of 2nd generation radiometal solution target. a. Included reflux chamber and smaller target volume to conserve precious target materials. b. Included aluminum for prototype and tantalum for working model. c. Included greater varicosities for improved heat transfer; and, (4) Construction of 2nd generation radiometal solution target started

  17. The Hurricane Imaging Radiometer: Present and Future

    Science.gov (United States)

    Miller, Timothy L.; James, M. W.; Roberts, J. B.; Biswas, S. K.; Cecil, D.; Jones, W. L.; Johnson, J.; Farrar, S.; Sahawneh, S.; Ruf, C. S.; hide

    2013-01-01

    The Hurricane Imaging Radiometer (HIRAD) is an airborne passive microwave radiometer designed to provide high resolution, wide swath imagery of surface wind speed in tropical cyclones from a low profile planar antenna with no mechanical scanning. Wind speed and rain rate images from HIRAD's first field campaign (GRIP, 2010) are presented here followed, by a discussion on the performance of the newly installed thermal control system during the 2012 HS3 campaign. The paper ends with a discussion on the next generation dual polarization HIRAD antenna (already designed) for a future system capable of measuring wind direction as well as wind speed.

  18. Dual color radiometer imagery and test results

    International Nuclear Information System (INIS)

    Silver, A.; Carlen, F.; Link, D.; Zegel, F.

    1989-01-01

    This paper presents a review of the technical characteristics of the Dual Color Radiometer and recent data and test results. The Dual Color Radiometer is a state-of-the-art device that provides simultaneous pixel to pixel registered thermal imagery in both the 3 to 5 and 8 to 12 micron regions. The device is unique in terms of its spatial and temperature resolution of less than 0.10 degrees C temperature and 0.10 milliradian spatial resolution. In addition, the device is tailored for use by the Automatic Target Recognizer (ATR) community

  19. Method of stably radiolabeling antibodies with technetium and rhenium

    International Nuclear Information System (INIS)

    Paik, C.H.; Reba, R.C.; Eckelman, W.C.

    1987-01-01

    A method is described for labeling antibodies or antibody fragments with radionuclides of technetium or rhenium to obtain stable labeling, comprising: reacting a reduced radioisotope of technetium or rhenium with an antibody or antibody fragment, or a diethylenetriaminepentaacetic acid conjugated antibody or antibody fragment, in the presence of free or carrier-bound diethylenetriaminepentaacetic acid (DTPA). The amount of DTPA is sufficient to substantially completely inhibit binding of the reduced technetium or rhenium to nonstable binding sites of the antibody or antibody fragment, or the DTPA-conjugated antibody or antibody fragment. The resultant stably labeled antibody or antibody fragment, or DTPA[conjugated antibody or antibody fragment is recovered

  20. Dynamic response of the thermometric net radiometer

    Science.gov (United States)

    J. D. Wilson; W. J. Massman; G. E. Swaters

    2009-01-01

    We computed the dynamic response of an idealized thermometric net radiometer, when driven by an oscillating net longwave radiation intended roughly to simulate rapid fluctuations of the radiative environment such as might be expected during field use of such devices. The study was motivated by curiosity as to whether non-linearity of the surface boundary conditions...

  1. Calibration of aerosol radiometers. Special aerosol sources

    International Nuclear Information System (INIS)

    Belkina, S.K.; Zalmanzon, Yu.E.; Kuznetsov, Yu.V.; Fertman, D.E.

    1988-01-01

    Problems of calibration of artificial aerosol radiometry and information-measurement systems of radiometer radiation control, in particular, are considered. Special aerosol source is suggested, which permits to perform certification and testing of aerosol channels of the systems in situ without the dismantling

  2. Balloon-borne radiometer profiler: Field observations

    International Nuclear Information System (INIS)

    Shaw, W.J.; Whiteman, C.D.; Anderson, G.A.; Alzheimer, J.M.; Hubbe, J.M.; Scott, K.A.

    1995-03-01

    This project involves the development of the capability of making routine soundings of broadband radiative fluxes and radiative flux divergences to heights of 1500m AGL. Described in this document are radiometers carried on a stabilized platform in a harness inserted in the tetherline of a tethered balloon meteriological sounding system. Field test results are given

  3. Dual Microwave Radiometer Experiment Field Campaign Report

    Energy Technology Data Exchange (ETDEWEB)

    Marchand, Roger [Univ. of Washington, Seattle, WA (United States)

    2017-09-01

    Passive microwave radiometers (MWRs) are the most commonly used and accurate instruments the U.S. Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) Research Facility has to retrieve cloud liquid water path (LWP). The MWR measurements (microwave radiances or brightness temperatures) are often used to derive LWP using climatological constraints, but are frequently also combined with measurements from radar and other instruments for cloud microphysical retrievals. Nominally this latter approach improves the retrieval of LWP and other cloud microphysical quantities (such as effective radius or number concentration), but this also means that when MWR data are poor, other cloud microphysical quantities are also negatively affected. Unfortunately, current MWR data is often contaminated by water on the MWR radome. This water makes a substantial contribution to the measured radiance and typically results in retrievals of cloud liquid water and column water vapor that are biased high. While it is obvious when the contamination by standing water is large (and retrieval biases are large), much of the time it is difficult to know with confidence that there is no contamination. At present there is no attempt to estimate or correct for this source of error, and identification of problems is largely left to users. Typically users are advised to simply throw out all data when the MWR “wet-window” resistance-based sensor indicates water is present, but this sensor is adjusted by hand and is known to be temperamental. In order to address this problem, a pair of ARM microwave radiometers was deployed to the University of Washington (UW) in Seattle, Washington, USA. The radiometers were operated such that one radiometer was scanned under a cover that (nominally) prevents this radiometer radome from gathering water and permits measurements away from zenith; while the other radiometer is operated normally – open or uncovered - with the radome exposed to the sky

  4. The JET ECE heterodyne radiometer and investigations of fast phenomena

    International Nuclear Information System (INIS)

    Bartlett, D.V.; Porte, L.

    1993-01-01

    In this paper, the design and performance characteristics of the JET heterodyne radiometer are reviewed, and some novel aspects of the instrument are described. Areas where the radiometer could benefit from further improvement are highlighted, and those improvements currently in progress are discussed. Some measurements which demonstrate the radiometer's power as a diagnostic of fast phenomena are presented. (orig.)

  5. Wideband filter radiometers for blackbody temperature measurements

    Science.gov (United States)

    Boivin, L. P.; Bamber, C.; Gaertner, A. A.; Gerson, R. K.; Woods, D. J.; Woolliams, E. R.

    2010-10-01

    The use of high-temperature blackbody (HTBB) radiators to realize primary spectral irradiance scales requires that the operating temperature of the HTBB be accurately determined. We have developed five filter radiometers (FRs) to measure the temperature of the National Research Council of Canada's HTBB. The FRs are designed to minimize sensitivity to ambient temperature fluctuations. They incorporate air-spaced colored glass filters and a Si photodiode detector that are housed in a cell whose temperature is controlled to ±0.1°C by means of annular thermoelectric elements at the front and rear of the cell. These wideband filter radiometers operate in four different wavelength bands. The spectral responsivity measurements were performed in an underfill geometry for a power-mode calibration that is traceable to NRC's cryogenic radiometer. The spectral temperature sensitivity of each of these FRs has been measured. The apertures for these FRs were cold-formed by swaging machine-cut apertures onto precision dowel pins. A description of the filter radiometer design, fabrication and testing, together with a detailed uncertainty analysis, is presented. We derive the equations that relate the spectral irradiance measured by the FRs to the spectral radiance and temperature of the HTBB, and deal specifically with the change of index of refraction over the path of the radiation from the interior of the HTBB to the FRs. We believe these equations are more accurate than recently published derivations. Our measurements of the operating temperature of our HTBB working at temperatures near 2500 K, 2700 K and 2900 K, together with measurements using a pyrometer, show agreement between the five filter radiometers and with the pyrometer to within the estimated uncertainties.

  6. Microfluidic radiolabeling of biomolecules with PET radiometals

    International Nuclear Information System (INIS)

    Zeng Dexing; Desai, Amit V.; Ranganathan, David; Wheeler, Tobias D.; Kenis, Paul J.A.; Reichert, David E.

    2013-01-01

    Introduction: A robust, versatile and compact microreactor has been designed, fabricated and tested for the labeling of bifunctional chelate conjugated biomolecules (BFC-BM) with PET radiometals. Methods: The developed microreactor was used to radiolabel a chelate, either 1,4,7,10-Tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) or 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) that had been conjugated to cyclo(Arg-Gly-Asp-DPhe-Lys) peptide, with both 64 Cu and 68 Ga respectively. The microreactor radiolabeling conditions were optimized by varying temperature, concentration and residence time. Results: Direct comparisons between the microreactor approach and conventional methods showed improved labeling yields and increased reproducibility with the microreactor under identical labeling conditions, due to enhanced mass and heat transfer at the microscale. More importantly, over 90% radiolabeling yields (incorporation of radiometal) were achieved with a 1:1 stoichiometry of bifunctional chelate biomolecule conjugate (BFC-BM) to radiometal in the microreactor, which potentially obviates extensive chromatographic purification that is typically required to remove the large excess of unlabeled biomolecule in radioligands prepared using conventional methods. Moreover, higher yields for radiolabeling of DOTA-functionalized BSA protein (Bovine Serum Albumin) were observed with 64 Cu/ 68 Ga using the microreactor, which demonstrates the ability to label both small and large molecules. Conclusions: A robust, reliable, compact microreactor capable of chelating radiometals with common chelates has been developed and validated. Based on our radiolabeling results, the reported microfluidic approach overall outperforms conventional radiosynthetic methods, and is a promising technology for the radiometal labeling of commonly utilized BFC-BM in aqueous solutions.

  7. Microfluidic radiolabeling of biomolecules with PET radiometals.

    Science.gov (United States)

    Zeng, Dexing; Desai, Amit V; Ranganathan, David; Wheeler, Tobias D; Kenis, Paul J A; Reichert, David E

    2013-01-01

    A robust, versatile and compact microreactor has been designed, fabricated and tested for the labeling of bifunctional chelate conjugated biomolecules (BFC-BM) with PET radiometals. The developed microreactor was used to radiolabel a chelate, either 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) or 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) that had been conjugated to cyclo(Arg-Gly-Asp-DPhe-Lys) peptide, with both ⁶⁴Cu and ⁶⁸Ga respectively. The microreactor radiolabeling conditions were optimized by varying temperature, concentration and residence time. Direct comparisons between the microreactor approach and conventional methods showed improved labeling yields and increased reproducibility with the microreactor under identical labeling conditions, due to enhanced mass and heat transfer at the microscale. More importantly, over 90% radiolabeling yields (incorporation of radiometal) were achieved with a 1:1 stoichiometry of bifunctional chelate biomolecule conjugate (BFC-BM) to radiometal in the microreactor, which potentially obviates extensive chromatographic purification that is typically required to remove the large excess of unlabeled biomolecule in radioligands prepared using conventional methods. Moreover, higher yields for radiolabeling of DOTA-functionalized BSA protein (Bovine Serum Albumin) were observed with ⁶⁴Cu/⁶⁸Ga using the microreactor, which demonstrates the ability to label both small and large molecules. A robust, reliable, compact microreactor capable of chelating radiometals with common chelates has been developed and validated. Based on our radiolabeling results, the reported microfluidic approach overall outperforms conventional radiosynthetic methods, and is a promising technology for the radiometal labeling of commonly utilized BFC-BM in aqueous solutions. Copyright © 2013 Elsevier Inc. All rights reserved.

  8. A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies.

    Science.gov (United States)

    Roy, Gargi; Martin, Tom; Barnes, Arnita; Wang, Jihong; Jimenez, Rod Brian; Rice, Megan; Li, Lina; Feng, Hui; Zhang, Shu; Chaerkady, Raghothama; Wu, Herren; Marelli, Marcello; Hatton, Diane; Zhu, Jie; Bowen, Michael A

    2018-04-01

    The conserved glycosylation site Asn 297 of a monoclonal antibody (mAb) can be decorated with a variety of sugars that can alter mAb pharmacokinetics and recruitment of effector proteins. Antibodies lacking the core fucose at Asn 297 (afucosylated mAbs) show enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) and increased efficacy. Here, we describe the development of a robust platform for the manufacture of afucosylated therapeutic mAbs by engineering a Chinese hamster ovary (CHO) host cell line to co-express a mAb with GDP-6-deoxy-D-lyxo-4-hexulose reductase (RMD), a prokaryotic enzyme that deflects an intermediate in the de novo synthesis of fucose to a dead-end product, resulting in the production of afucosylated mAb (GlymaxX™ Technology, ProBioGen). Expression of the mAb and RMD genes was coordinated by co-transfection of separate mAb and RMD vectors or use of an internal ribosome entry site (IRES) element to link the translation of RMD with either the glutamine synthase selection marker or the mAb light chain. The GS-IRES-RMD vector format was more suitable for the rapid generation of high yielding cell lines, secreting afucosylated mAb with titers exceeding 6.0 g/L. These cell lines maintained production of afucosylated mAb over 60 generations, ensuring their suitability for use in large-scale manufacturing. The afucosylated mAbs purified from these RMD-engineered cell lines showed increased binding in a CD16 cellular assay, demonstrating enhancement of ADCC compared to fucosylated control mAb. Furthermore, the afucosylation in these mAbs could be controlled by simple addition of L-fucose in the culture medium, thereby allowing the use of a single cell line for production of the same mAb in fucosylated and afucosylated formats for multiple therapeutic indications.

  9. Quality not quantity for transglutaminase antibody 2: the performance of an endomysial and tissue transglutaminase test in screening coeliac disease remains stable over time.

    Science.gov (United States)

    Swallow, K; Wild, G; Sargur, R; Sanders, D S; Aziz, I; Hopper, A D; Egner, W

    2013-01-01

    National Institute of Clinical Excellence (NICE) and European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) guidance for the diagnosis of coeliac disease has been published. However, there is some controversy regarding the advice on the use of stratifying levels of immunoglobulin (IgA) tissue transglutaminase antibody (TG2) test positivity in the absence of test standardization and the vagueness of the indication to test equivocal samples. Using repeat service audit, we demonstrate that a combination of TG2 followed by IgA endomysial antibodies (EMA) is the best strategy for all degrees of mucosal abnormality using our test combination. Reliance upon immunoassay titre is not as effective, and cannot be applied consistently across populations in the absence of assay standardization. Guidelines advocating the use of tests should involve experts in laboratory diagnostics and external quality assurance to ensure that errors of generalization do not occur and that test performance is achievable in routine diagnostic use. © 2012 British Society for Immunology.

  10. Metal chelate conjugated monoclonal antibodies, wherein the metal is an α emitter

    International Nuclear Information System (INIS)

    Gansow, O.A.; Strand, M.

    1984-01-01

    Methods of manufacturing and purifying metal chelate conjugated monoclonal antibodies are described, wherein the chelated metal emits alpha radiation. The conjugates are suited for therapeutic uses being substantially free of nonchelated radiometal. (author)

  11. Antibodies and Selection of Monoclonal Antibodies.

    Science.gov (United States)

    Hanack, Katja; Messerschmidt, Katrin; Listek, Martin

    Monoclonal antibodies are universal binding molecules with a high specificity for their target and are indispensable tools in research, diagnostics and therapy. The biotechnological generation of monoclonal antibodies was enabled by the hybridoma technology published in 1975 by Köhler and Milstein. Today monoclonal antibodies are used in a variety of applications as flow cytometry, magnetic cell sorting, immunoassays or therapeutic approaches. First step of the generation process is the immunization of the organism with appropriate antigen. After a positive immune response the spleen cells are isolated and fused with myeloma cells in order to generate stable, long-living antibody-producing cell lines - hybridoma cells. In the subsequent identification step the culture supernatants of all hybridoma cells are screened weekly for the production of the antibody of interest. Hybridoma cells producing the antibody of interest are cloned by limited dilution till a monoclonal hybridoma is found. This is a very time-consuming and laborious process and therefore different selection strategies were developed since 1975 in order to facilitate the generation of monoclonal antibodies. Apart from common automation of pipetting processes and ELISA testing there are some promising approaches to select the right monoclonal antibody very early in the process to reduce time and effort of the generation. In this chapter different selection strategies for antibody-producing hybridoma cells are presented and analysed regarding to their benefits compared to conventional limited dilution technology.

  12. The Impact of Indoor and Outdoor Radiometer Calibration on Solar Measurements

    Energy Technology Data Exchange (ETDEWEB)

    Habte, Aron; Sengupta, Manajit; Andreas, Afshin; Reda, Ibrahim; Robinson, Justin

    2016-06-02

    This study addresses the effect of calibration methodologies on calibration responsivities and the resulting impact on radiometric measurements. The calibration responsivities used in this study are provided by NREL's broadband outdoor radiometer calibration (BORCAL) and a few prominent manufacturers. The BORCAL method provides outdoor calibration responsivity of pyranometers and pyrheliometers at a 45 degree solar zenith angle and responsivity as a function of solar zenith angle determined by clear-sky comparisons to reference irradiance. The BORCAL method also employs a thermal offset correction to the calibration responsivity of single-black thermopile detectors used in pyranometers. Indoor calibrations of radiometers by their manufacturers are performed using a stable artificial light source in a side-by-side comparison of the test radiometer under calibration to a reference radiometer of the same type. These different methods of calibration demonstrated 1percent to 2 percent differences in solar irradiance measurement. Analyzing these values will ultimately enable a reduction in radiometric measurement uncertainties and assist in developing consensus on a standard for calibration.

  13. Radiometers for radon concentration in air

    International Nuclear Information System (INIS)

    Bartak, J.; Machaj, B.; Pienkos, J.P.

    2002-01-01

    Constant grow of science and technology stimulates development of new improved measuring tools. New measuring demand arise also in radon concentration measurements. Varying rock stress and rock cracks influencing radon emanation encouraged research aimed at use of this phenomenon to predict crumps of mine formation among others based on variation of radon emanation. A measuring set was developed in the Institute of Nuclear Chemistry and Technology enabling long term monitoring of radon concentration in mine bore-hole. The set consists probe and probe controller. Detection threshold of the probe is 230 Bq/m 3 . The set can operate in the environment with methane explosion hazard. A radiometer employing Lucas cell as radiation detector for radon concentration in air was also developed its detection threshold is approx. 10 Bq/m 3 . Replaceable Lucas cell of the radiometer allows for measurement of high as well as low radon concentration in short time interval. (author)

  14. Intersatellite Calibration of Microwave Radiometers for GPM

    Science.gov (United States)

    Wilheit, T. T.

    2010-12-01

    The aim of the GPM mission is to measure precipitation globally with high temporal resolution by using a constellation of satellites logically united by the GPM Core Satellite which will be in a non-sunsynchronous, medium inclination orbit. The usefulness of the combined product depends on the consistency of precipitation retrievals from the various microwave radiometers. The calibration requirements for this consistency are quite daunting requiring a multi-layered approach. The radiometers can vary considerably in their frequencies, view angles, polarizations and spatial resolutions depending on their primary application and other constraints. The planned parametric algorithms will correct for the varying viewing parameters, but they are still vulnerable to calibration errors, both relative and absolute. The GPM Intersatellite Calibration Working Group (aka X-CAL) will adjust the calibration of all the radiometers to a common consensus standard for the GPM Level 1C product to be used in precipitation retrievals. Finally, each Precipitation Algorithm Working Group must have its own strategy for removing the residual errors. If the final adjustments are small, the credibility of the precipitation retrievals will be enhanced. Before intercomparing, the radiometers must be self consistent on a scan-wise and orbit-wise basis. Pre-screening for this consistency constitutes the first step in the intercomparison. The radiometers are then compared pair-wise with the microwave radiometer (GMI) on the GPM Core Satellite. Two distinct approaches are used for sake of cross-checking the results. On the one hand, nearly simultaneous observations are collected at the cross-over points of the orbits and the observations of one are converted to virtual observations of the other using a radiative transfer model to permit comparisons. The complementary approach collects histograms of brightness temperature from each instrument. In each case a model is needed to translate the

  15. A radiometer for stochastic gravitational waves

    International Nuclear Information System (INIS)

    Ballmer, Stefan W

    2006-01-01

    The LIGO Scientific Collaboration recently reported a new upper limit on an isotropic stochastic background of gravitational waves obtained based on the data from the third LIGO science run (S3). Here I present a new method for obtaining directional upper limits on stochastic gravitational waves that essentially implements a gravitational wave radiometer. The LIGO Scientific Collaboration intends to use this method for future LIGO science runs

  16. LAMMR: A new generation satellite microwave radiometer - Its concepts and capabilities. [Large Antenna Multichannel Microwave Radiometer

    Science.gov (United States)

    Walton, W. T.; Wilheit, T. T.

    1981-01-01

    Definition studies and baseline design are summarized for the proposed, and now discontinued, LAMMR. The instrument is an offset parabolic reflector with Cassegrain feeds. The three-meter aperture reflector, to be constructed using graphite-epoxy technology, rotates continuously at 0.833 rps. The scan drive subsystem includes momentum compensation for the rotating mass which includes the reflector, the support arm and Cassegrain subreflector, feed horns and radiometer. Two total power radiometers are recommended for each frequency, one each for horizontal and vertical polarizations. The selection plan, definition study specifications, LAMMR performance specifications, and predicted accuracies and resolutions after processing are shown.

  17. A horizontal vane radiometer: experiment, theory and simulation

    OpenAIRE

    Wolfe, David; Lazarra, Andres; Garcia, Alejandro

    2015-01-01

    The existence of two motive forces on a Crookes radiometer has complicated the investigation of either force independently. The thermal creep shear force in particular has been subject to differing interpretations of the direction in which it acts and its order of magnitude. In this article we provide a horizontal vane radiometer design which isolates the thermal creep shear force. The horizontal vane radiometer is explored through experiment, kinetic theory, and the Direct Simulation Monte C...

  18. Remote Sensing of Surface Soil Moisture using Semi-Concurrent Radar and Radiometer Observations

    Science.gov (United States)

    Li, L.; Ouellette, J. D.; Colliander, A.; Cosh, M. H.; Caldwell, T. G.; Walker, J. P.

    2017-12-01

    Radar backscatter and radiometer brightness temperature both have well-documented sensitivity to surface soil moisture, particularly in the microwave regime. While radiometer-derived soil moisture retrievals have been shown to be stable and accurate, they are only available at coarse spatial resolutions on the order of tens of kilometers. Backscatter from Synthetic Aperture Radar (SAR) is similarly sensitive to soil moisture but can yield higher spatial resolutions, with pixel sizes about an order of magnitude smaller. Soil moisture retrieval from radar backscatter is more difficult, however, due to the combined sensitivity of radar scattering to surface roughness, vegetation structure, and soil moisture. The algorithm uses a time-series of SAR data to retrieval soil moisture information, constraining the SAR-derived soil moisture estimates with radiometer observations. This effectively combines the high spatial resolution offered by SAR with the precision offered by passive radiometry. The algorithm is a change detection approach which maps changes in the radar backscatter to changes in surface soil moisture. This new algorithm differs from existing retrieval techniques in that it does not require ancillary vegetation information, but assumes vegetation and surface roughness are stable between pairs of consecutive radar overpasses. Furthermore, this method does not require a radar scattering model for the vegetation canopy, nor the use of a training data set. The algorithm works over a long time series, and is constrained by hard bounds which are defined using a coarse-resolution radiometer soil moisture product. The presentation will include soil moisture retrievals from Soil Moisture Active/Passive (SMAP) SAR data. Two sets of optimization bounds will constrain the radar change detection algorithm: one defined by SMAP radiometer retrievals and one defined by WindSat radiometer retrievals. Retrieved soil moisture values will be presented on a world map and will

  19. GPM GROUND VALIDATION DUAL POLARIZATION RADIOMETER GCPEX V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The GPM Ground Validation Dual Polarization Radiometer GCPEx dataset provides brightness temperature measurements at frequencies 90 GHz (not polarized) and 150 GHz...

  20. Antimitochondrial antibody

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003529.htm Antimitochondrial antibody To use the sharing features on this page, please enable JavaScript. Antimitochondrial antibodies (AMA) are substances ( antibodies ) that form against mitochondria. ...

  1. Calibration of the TUD Ku-band Synthetic Aperture Radiometer

    DEFF Research Database (Denmark)

    Laursen, Brian; Skou, Niels

    1995-01-01

    The TUD Synthetic Aperture Radiometer is a 2-channel demonstration model that can simulate a thinned aperture radiometer having an unfilled aperture consisting of several small antenna elements. Aperture synthesis obtained by interferometric measurements using the antenna elements in pairs, follo...

  2. The DC-8 Submillimeter-Wave Cloud Ice Radiometer

    Science.gov (United States)

    Walter, Steven J.; Batelaan, Paul; Siegel, Peter; Evans, K. Franklin; Evans, Aaron; Balachandra, Balu; Gannon, Jade; Guldalian, John; Raz, Guy; Shea, James

    2000-01-01

    An airborne radiometer is being developed to demonstrate the capability of radiometry at submillimeter-wavelengths to characterize cirrus clouds. At these wavelengths, cirrus clouds scatter upwelling radiation from water vapor in the lower troposphere. Radiometric measurements made at multiple widely spaced frequencies permit flux variations caused by changes in scattering due to crystal size to be distinguished from changes in cloud ice content. Measurements at dual polarizations can also be used to constrain the mean crystal shape. An airborne radiometer measuring the upwelling submillimeter-wave flux should then able to retrieve both bulk and microphysical cloud properties. The radiometer is being designed to make measurements at four frequencies (183 GHz, 325 GHz, 448 GHz, and 643 GHz) with dual-polarization capability at 643 GHz. The instrument is being developed for flight on NASA's DC-8 and will scan cross-track through an aircraft window. Measurements with this radiometer in combination with independent ground-based and airborne measurements will validate the submillimeter-wave radiometer retrieval techniques. The goal of this effort is to develop a technique to enable spaceborne characterization of cirrus, which will meet a key climate measurement need. The development of an airborne radiometer to validate cirrus retrieval techniques is a critical step toward development of spaced-based radiometers to investigate and monitor cirrus on a global scale. The radiometer development is a cooperative effort of the University of Colorado, Colorado State University, Swales Aerospace, and Jet Propulsion Laboratory and is funded by the NASA Instrument Incubator Program.

  3. L-Band Polarimetric Correlation Radiometer with Subharmonic Sampling

    DEFF Research Database (Denmark)

    Rotbøll, Jesper; Søbjærg, Sten Schmidl; Skou, Niels

    2001-01-01

    A novel L-band radiometer trading analog complexity for digital ditto has been designed and built. It is a fully polarimetric radiometer of the correlation type and it is based on the sub-harmonic sampling principle in which the L-band signal is directly sampled by a fast A to D converter...

  4. Novel multi-beam radiometers for accurate ocean surveillance

    DEFF Research Database (Denmark)

    Cappellin, C.; Pontoppidan, K.; Nielsen, P. H.

    2014-01-01

    Novel antenna architectures for real aperture multi-beam radiometers providing high resolution and high sensitivity for accurate sea surface temperature (SST) and ocean vector wind (OVW) measurements are investigated. On the basis of the radiometer requirements set for future SST/OVW missions...

  5. A novel L-band polarimetric radiometer featuring subharmonic sampling

    DEFF Research Database (Denmark)

    Rotbøll, J.; Søbjærg, Sten Schmidl; Skou, Niels

    2003-01-01

    A novel L-band radiometer trading analog components for digital circuits has been designed, built and operated. It is a fully polarimetric radiometer of the correlation type, and it is based on the subharmonic sampling principle in which the L-band signal is directly sampled by a fast A to D...

  6. Etched track radiometers in radon measurements: a review

    CERN Document Server

    Nikolaev, V A

    1999-01-01

    Passive radon radiometers, based on alpha particle etched track detectors, are very attractive for the assessment of radon exposure. The present review considers various devices used for measurement of the volume activity of radon isotopes and their daughters and determination of equilibrium coefficients. Such devices can be classified into 8 groups: (i) open or 'bare' detectors, (ii) open chambers, (iii) sup 2 sup 2 sup 2 Rn chambers with an inlet filter, (iv) advanced sup 2 sup 2 sup 2 Rn radiometers, (v) multipurpose radiometers, (vi) radiometers based on a combination of etched track detectors and an electrostatic field, (vii) radiometers based on etched track detectors and activated charcoal and (viii) devices for the measurement of radon isotopes and/or radon daughters by means of track parameter measurements. Some of them such as the open detector and the chamber with an inlet filter have a variety of modifications and are applied widely both in geophysical research and radon dosimetric surveys. At the...

  7. MCM Polarimetric Radiometers for Planar Arrays

    Science.gov (United States)

    Kangaslahti, Pekka; Dawson, Douglas; Gaier, Todd

    2007-01-01

    A polarimetric radiometer that operates at a frequency of 40 GHz has been designed and built as a prototype of multiple identical units that could be arranged in a planar array for scientific measurements. Such an array is planned for use in studying the cosmic microwave background (CMB). All of the subsystems and components of this polarimetric radiometer are integrated into a single multi-chip module (MCM) of substantially planar geometry. In comparison with traditional designs of polarimetric radiometers, the MCM design is expected to greatly reduce the cost per unit in an array of many such units. The design of the unit is dictated partly by a requirement, in the planned CMB application, to measure the Stokes parameters I, Q, and U of the CMB radiation with high sensitivity. (A complete definition of the Stokes parameters would exceed the scope of this article. In necessarily oversimplified terms, I is a measure of total intensity of radiation, while Q and U are measures of the relationships between the horizontally and vertically polarized components of radiation.) Because the sensitivity of a single polarimeter cannot be increased significantly, the only way to satisfy the high-sensitivity requirement is to make a large array of polarimeters that operate in parallel. The MCM includes contact pins that can be plugged into receptacles on a standard printed-circuit board (PCB). All of the required microwave functionality is implemented within the MCM; any required supporting non-microwave ("back-end") electronic functionality, including the provision of DC bias and control signals, can be implemented by standard PCB techniques. On the way from a microwave antenna to the MCM, the incoming microwave signal passes through an orthomode transducer (OMT), which splits the radiation into an h + i(nu) beam and an h - i(nu) beam (where, using complex-number notation, h denotes the horizontal component, nu denotes the vertical component, and +/-i denotes a +/-90deg phase

  8. Radiometallating antibodies and autoantigenic peptides

    International Nuclear Information System (INIS)

    Mercer-Smith, J.A.; Lewis, D.; Cole, D.A.; Newmyer, S.L.; Schulte, L.D.; Mixon, P.L.; Schreyer, S.A.; Burns, T.P.; Roberts, J.C.; Figard, S.D.; McCormick, D.J.; Lennon, V.A.; Hayashi, M.; Lavallee, D.K.

    1991-01-01

    We have developed methods to radiolabel large molecules, using porphyrins as bifunctional chelating agents for radiometals. The porphyrins are substituted with an N- benzyl group to activate them for radiometallation under mild reaction conditions. Porphyrins that have one functional group for covalent attachment to other molecules cannot cause crosslinking. We have examined the labeling chemistry for antibodies and have developed methods to label smaller biologically active molecules, such as autoantigenic peptides (fragments of the acetylcholine receptor), which are pertinent to myasthenia gravis research. The methods of covalent attachment of these bifunctional chelating agents to large molecules, the radiometallation chemistry, and biological characterization of the radiolabeled compounds will be discussed

  9. Microwave integrated circuit radiometer front-ends for the Push Broom Microwave Radiometer

    Science.gov (United States)

    Harrington, R. F.; Hearn, C. P.

    1982-01-01

    Microwave integrated circuit front-ends for the L-band, S-band and C-band stepped frequency null-balanced noise-injection Dicke-switched radiometer to be installed in the NASA Langley airborne prototype Push Broom Microwave Radiometer (PBMR) are described. These front-ends were developed for the fixed frequency of 1.413 GHz and the variable frequencies of 1.8-2.8 GHz and 3.8-5.8 GHz. Measurements of the noise temperature of these units were made at 55.8 C, and the results of these tests are given. While the overall performance was reasonable, improvements need to be made in circuit losses and noise temperatures, which in the case of the C-band were from 1000 to 1850 K instead of the 500 K specified. Further development of the prototypes is underway to improve performance and extend the frequency range.

  10. Mapping global precipitation with satellite borne microwave radiometer and infrared radiometer using Kalman filter

    International Nuclear Information System (INIS)

    Noda, S.; Sasashige, K.; Katagami, D.; Ushio, T.; Kubota, T.; Okamoto, K.; Iida, Y.; Kida, S.; Shige, S.; Shimomura, S.; Aonashi, K.; Inoue, T.; Morimoto, T.; Kawasaki, Z.

    2007-01-01

    Estimates of precipitation at a high time and space resolution are required for many important applications. In this paper, a new global precipitation map with high spatial (0.1 degree) and temporal (1 hour) resolution using Kalman filter technique is presented and evaluated. Infrared radiometer data, which are available globally nearly everywhere and nearly all the time from geostationary orbit, are used with the several microwave radiometers aboard the LEO satellites. IR data is used as a means to move the precipitation estimates from microwave observation during periods when microwave data are not available at a given location. Moving vector is produced by computing correlations on successive images of IR data. When precipitation is moved, the Kalman filter is applied for improving the moving technique in this research. The new approach showed a better score than the technique without Kalman filter. The correlation coefficient was 0.1 better than without the Kalman filter about 6 hours after the last microwave overpasses, and the RMS error was improved about 0.1 mm/h with the Kalman filter technique. This approach is unique in that 1) the precipitation estimates from the microwave radiometer is mainly used, 2) the IR temperature in every hour is also used for the precipitation estimates based on the Kalman filter theory

  11. Experimental verification of self-calibration radiometer based on spontaneous parametric downconversion

    Science.gov (United States)

    Gao, Dongyang; Zheng, Xiaobing; Li, Jianjun; Hu, Youbo; Xia, Maopeng; Salam, Abdul; Zhang, Peng

    2018-03-01

    Based on spontaneous parametric downconversion process, we propose a novel self-calibration radiometer scheme which can self-calibrate the degradation of its own response and ultimately monitor the fluctuation of a target radiation. Monitor results were independent of its degradation and not linked to the primary standard detector scale. The principle and feasibility of the proposed scheme were verified by observing bromine-tungsten lamp. A relative standard deviation of 0.39 % was obtained for stable bromine-tungsten lamp. Results show that the proposed scheme is advanced of its principle. The proposed scheme could make a significant breakthrough in the self-calibration issue on the space platform.

  12. Special aerosol sources for certification and test of aerosol radiometers

    International Nuclear Information System (INIS)

    Belkina, S.K.; Zalmanzon, Y.E.; Kuznetsov, Y.V.; Rizin, A.I.; Fertman, D.E.

    1991-01-01

    The results are presented of the development and practical application of new radionuclide source types (Special Aerosol Sources (SAS)), that meet the international standard recommendations, which are used for certification and test of aerosol radiometers (monitors) using model aerosols of plutonium-239, strontium-yttrium-90 or uranium of natural isotope composition and certified against Union of Soviet Socialist Republics USSR national radioactive aerosol standard or by means of a reference radiometer. The original technology for source production allows the particular features of sampling to be taken into account as well as geometry and conditions of radionuclides radiation registration in the sample for the given type of radiometer. (author)

  13. Special aerosol sources for certification and test of aerosol radiometers

    Energy Technology Data Exchange (ETDEWEB)

    Belkina, S.K.; Zalmanzon, Y.E.; Kuznetsov, Y.V.; Rizin, A.I.; Fertman, D.E. (Union Research Institute of Instrumentation, Moscow (USSR))

    1991-01-01

    The results are presented of the development and practical application of new radionuclide source types (Special Aerosol Sources (SAS)), that meet the international standard recommendations, which are used for certification and test of aerosol radiometers (monitors) using model aerosols of plutonium-239, strontium-yttrium-90 or uranium of natural isotope composition and certified against Union of Soviet Socialist Republics USSR national radioactive aerosol standard or by means of a reference radiometer. The original technology for source production allows the particular features of sampling to be taken into account as well as geometry and conditions of radionuclides radiation registration in the sample for the given type of radiometer. (author).

  14. Multifilter Rotating Shadowband Radiometer (MFRSR) Handbook With subsections for derivative instruments: Multifilter Radiometer (MFR) Normal Incidence Multifilter Radiometer (NIMFR)

    Energy Technology Data Exchange (ETDEWEB)

    Hodges, Gary B. [National Oceanic and Atmospheric Administration (NOAA), Boulder, CO (United States). Earth System Research Lab.; Michalsky, Joseph J. [National Oceanic and Atmospheric Administration (NOAA), Boulder, CO (United States). Earth System Research Lab.

    2016-03-01

    The visible Multifilter Rotating Shadowband Radiometer (MFRSR) is a passive instrument that measures global and diffuse components of solar irradiance at six narrowband channels and one open, or broadband, channel (Harrison et al. 1994). Direct irradiance is not a primary measurement, but is calculated using diffuse and global measurements. To collect one data record, the MFRSR takes measurements at four different shadowband positions. The first measurement is taken with the shadowband in the nadir (home) position. The next three measurements are, in order, the first side-band, sun-blocked, and second side-band. The side-band measurements are used to correct for the portion of the sky obscured by the shadowband. The nominal wavelengths of the narrowband channels are 415, 500, 615, 673, 870, and 940 nm. From such measurements, one may infer the atmosphere’s aerosol optical depth at each wavelength. In turn, these optical depths may be used to derive information about the column abundances of ozone and water vapor (Michalsky et al. 1995), as well as aerosol (Harrison and Michalsky 1994) and other atmospheric constituents.

  15. CAROLS: A New Airborne L-Band Radiometer for Ocean Surface and Land Observations

    DEFF Research Database (Denmark)

    Zribi, Mehrez; Parde, Mickael; Boutin, Jacquline

    2011-01-01

    The "Cooperative Airborne Radiometer for Ocean and Land Studies" (CAROLS) L-Band radiometer was designed and built as a copy of the EMIRAD II radiometer constructed by the Technical University of Denmark team. It is a fully polarimetric and direct sampling correlation radiometer. It is installed ...

  16. Construction and calibration of solar radiometers: pyranometer and pyrheliometer

    International Nuclear Information System (INIS)

    Escobedo, J.F.; Passos, E.F.; Souza, M.F. de

    1988-01-01

    This paper reports the construction and development of solar radiometers and discusses some characteristic parameters such as linearity, sensitivity and time constant, using an Eppley black-and-white pyranometer as reference. (author) [pt

  17. The development of the advanced cryogenic radiometer facility at NRC

    Science.gov (United States)

    Gamouras, A.; Todd, A. D. W.; Côté, É.; Rowell, N. L.

    2018-02-01

    The National Research Council (NRC) of Canada has established a next generation facility for the primary realization of optical radiant power. The main feature of this facility is a new cryogenic electrical substitution radiometer with a closed-cycle helium cryocooler. A monochromator-based approach allows for detector calibrations at any desired wavelength. A custom-designed motion apparatus includes two transfer standard radiometer mounting ports which has increased our measurement capability by allowing the calibration of two photodetectors in one measurement cycle. Measurement uncertainties have been improved through several upgrades, including newly designed and constructed transimpedance amplifiers for the transfer standard radiometers, and a higher power broadband light source. The most significant improvements in uncertainty arise from the enhanced characteristics of the new cryogenic radiometer including its higher cavity absorptance and reduced non-equivalence effects.

  18. Hurricane Satellite (HURSAT) from Advanced Very High Resolution Radiometer (AVHRR)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Huricane Satellite (HURSAT)-Advanced Very High Resolution Radiometer (AVHRR) is used to extend the HURSAT data set such that appling the Objective Dvorak technique...

  19. Effect of Chamber Wall Proximity on Radiometer Force Production (Preprint)

    National Research Council Canada - National Science Library

    Selden, N. P; Gimelshein, N. E; Gimelshein, S. F; Ketsdever, A. D

    2008-01-01

    ... on a given radiometer configuration in both the free molecule and transitional regimes. The contribution of the chamber walls to both the flowfield structure and radiometric force production were examined for helium, argon, and nitrogen test gases...

  20. Characterization of a Compact Water Vapor Radiometer

    Science.gov (United States)

    Gill, Ajay; Selina, Rob

    2018-01-01

    We report on laboratory test results of the Compact Water Vapor Radiometer (CWVR) prototype for the Karl G. Jansky Very Large Array (VLA), a five-channel design centered around the 22 GHz water vapor line. Fluctuations in perceptible water vapor cause fluctuations in atmospheric brightness emission, which are assumed to be proportional to phase fluctuations of the astronomical signal seen by an antenna. The design is intended to support empirical radiometric phase corrections for each baseline in the array.The dynamic range, channel isolation, and gain stability of the device were characterized. The device has a useful dynamic range of order 18 dB after calibration, and the CWVR channel isolation requirement of test, the diode detectors were operated in the square-law region, and a K-band noise diode was used as the broadband input power source to the CWVR over a period of 64 hours. Results indicate that the fluctuations in output counts are negatively correlated to the CWVR enclosure ambient temperature, with a change of ~ 405 counts per 1° C change in temperature.A correction for the CWVR ambient temperature makes a considerable improvement in stability for τ > 102.6 sec. With temperature corrections, the single channel and channel difference gain stability per channel is test results indicate that the CWVR meets required specifications for dynamic range, channel isolation, and gain stability in order to proceed with testing on a pair of VLA antennas.

  1. Multifilter Rotating Shadowband Radiometer (MFRSR) Handbook

    Energy Technology Data Exchange (ETDEWEB)

    Hodges, GB; Michalsky, JJ

    2011-02-07

    The visible Multifilter Rotating Shadowband Radiometer (MFRSR) is a passive instrument that measures global and diffuse components of solar irradiance at six narrowband channels and one open, or broadband, channel (Harrison et al. 1994). Direct irradiance is not a primary measurement, but is calculated using the diffuse and global measurements. To collect one data record, the MFRSR takes measurements at four different shadowband positions. The first measurement is taken with the shadowband in the nadir (home) position. The next three measurements are, in order, the first side-band, sun-blocked, and second side-band. The side-band measurements are used to correct for the portion of the sky obscured by the shadowband. The nominal wavelengths of the narrowband channels are 415, 500, 615, 673, 870, and 940 nm. From such measurements, one may infer the atmosphere's aerosol optical depth at each wavelength. In turn, these optical depths may be used to derive information about the column abundances of ozone and water vapor (Michalsky et al. 1995), as well as aerosol (Harrison and Michalsky 1994) and other atmospheric constituents.

  2. Antibody biotechnology

    African Journals Online (AJOL)

    STORAGESEVER

    2009-07-06

    Jul 6, 2009 ... Another milestone in the history of antibodies was the work of Porter and Edelman ... transgenic animals (Lonberg et al., 1994; Green et al.,. 1994) or .... create and to screen human recombinant antibodies libraries, that is ...

  3. Stable isotopes

    International Nuclear Information System (INIS)

    Evans, D.K.

    1986-01-01

    Seventy-five percent of the world's stable isotope supply comes from one producer, Oak Ridge Nuclear Laboratory (ORNL) in the US. Canadian concern is that foreign needs will be met only after domestic needs, thus creating a shortage of stable isotopes in Canada. This article describes the present situation in Canada (availability and cost) of stable isotopes, the isotope enrichment techniques, and related research programs at Chalk River Nuclear Laboratories (CRNL)

  4. Antithyroid microsomal antibody

    Science.gov (United States)

    Thyroid antimicrosomal antibody; Antimicrosomal antibody; Microsomal antibody; Thyroid peroxidase antibody; TPOAb ... Granulomatous thyroiditis Hashimoto thyroiditis High levels of these antibodies have also been linked with an increased risk ...

  5. Sources of errors in the measurements of underwater profiling radiometer

    Digital Repository Service at National Institute of Oceanography (India)

    Silveira, N.; Suresh, T.; Talaulikar, M.; Desa, E.; Matondkar, S.G.P.; Lotlikar, A.

    to meet the stringent quality requirements of marine optical data for satellite ocean color sensor validation, development of algorithms and other related applications, it is very essential to take great care while measuring these parameters. There are two... of the pelican hook. The radiometer dives vertically and the cable is paid out with less tension, keeping in tandem with the descent of the radiometer while taking care to release only the required amount of cable. The operation of the release mechanism lever...

  6. Measurement of radiosity coefficient by means of an infrared radiometer

    Energy Technology Data Exchange (ETDEWEB)

    Okamoto, Yoshizo; Kaminaga, Fumito; Osakabe, Masahiro; Maekawa, Katsuhiro [Ibaraki Univ., Hitachi (Japan). Faculty of Engineering; Ishii, Toshimitsu; Ouoka, Norikazu; Etou, Motokuni

    1991-02-01

    An infrared radiometer has been used for measuring and visualizing the radiation temperature distribution of a surface in many fields. Measured radiation energy by the radiometer is a summation of an emitted radiation and a reflection, which is called a radiosity flux. The present paper shows the characteristics of the radiosity of tested materials. The infrared sensor in used to measure the erosion rate of the graphite by ion beam injection and the temperature distribution of a cutter. (author).

  7. Measurement of radiosity coefficient by means of an infrared radiometer

    International Nuclear Information System (INIS)

    Okamoto, Yoshizo; Kaminaga, Fumito; Osakabe, Masahiro; Maekawa, Katsuhiro; Ishii, Toshimitsu; Ouoka, Norikazu; Etou, Motokuni.

    1991-01-01

    An infrared radiometer has been used for measuring and visualizing the radiation temperature distribution of a surface in many fields. Measured radiation energy by the radiometer is a summation of an emitted radiation and a reflection, which is called a radiosity flux. The present paper shows the characteristics of the radiosity of tested materials. The infrared sensor in used to measure the erosion rate of the graphite by ion beam injection and the temperature distribution of a cutter. (author)

  8. A cost effective total power radiometer package for atmospheric research

    International Nuclear Information System (INIS)

    Lyons, B.N.; Kelly, W.M.; Vizard, D.R.; Lidholm, U.S.

    1993-01-01

    Millimeter wave radiometers are being increasingly used for plasma diagnostics and remote sensing applications. To date however the widespread use of such systems, particularly for applications requiring frequency coverage above 100 GHz, have been inhibited by the lack of availability of an appropriately specified commercial package. This paper outlines the design and construction of such a radiometer package and gives details of results obtained to date

  9. Thyroid Antibodies

    Science.gov (United States)

    ... PF4 Antibody Hepatitis A Testing Hepatitis B Testing Hepatitis C Testing HER2/neu Herpes Testing High-sensitivity C-reactive Protein (hs-CRP) Histamine Histone Antibody HIV Antibody and HIV Antigen (p24) HIV Antiretroviral Drug Resistance Testing, Genotypic HIV Viral Load HLA Testing HLA- ...

  10. Optimization of procedure for calibration with radiometer/photometer

    International Nuclear Information System (INIS)

    Detilly, Isabelle

    2009-01-01

    A test procedure for the radiometer/photometer calibrations mark International Light at the Laboratorio de Fotometria y Tecnologia Laser (LAFTA) de la Escuela de Ingenieria Electrica de la Universidad de Costa Rica is established. Two photometric banks are used as experimental set and two calibrations were performed of the International Light. A basic procedure established in the laboratory, is used for calibration from measurements of illuminance and luminous intensity. Some dependent variations of photometric banks used in the calibration process, the programming of the radiometer/photometer and the applied methodology showed the results. The procedure for calibration with radiometer/photometer can be improved by optimizing the programming process of the measurement instrument and possible errors can be minimized by using the recommended procedure. (author) [es

  11. Antiprothrombin Antibodies

    Directory of Open Access Journals (Sweden)

    Polona Žigon

    2015-05-01

    Full Text Available In patients with the antiphospholipid syndrome (APS, the presence of a group of pathogenic autoantibodies called antiphospholipid antibodies causes thrombosis and pregnancy complications. The most frequent antigenic target of antiphospholipid antibodies are phospholipid bound β2-glycoprotein 1 (β2GPI and prothrombin. The international classification criteria for APS connect the occurrence of thrombosis and/or obstetric complications together with the persistence of lupus anticoagulant, anti-cardiolipin antibodies (aCL and antibodies against β2GPI (anti-β2GPI into APS. Current trends for the diagnostic evaluation of APS patients propose determination of multiple antiphospholipid antibodies, among them also anti-prothrombin antibodies, to gain a common score which estimates the risk for thrombosis in APS patients. Antiprothrombin antibodies are common in APS patients and are sometimes the only antiphospholipid antibodies being elevated. Methods for their determination differ and have not yet been standardized. Many novel studies confirmed method using phosphatidylserine/prothrombin (aPS/PT ELISA as an antigen on solid phase encompass higher diagnostic accuracy compared to method using prothrombin alone (aPT ELISA. Our research group developed an in-house aPS/PT ELISA with increased analytical sensitivity which enables the determination of all clinically relevant antiprothrombin antibodies. aPS/PT exhibited the highest percentage of lupus anticoagulant activity compared to aCL and anti-β2GPI. aPS/PT antibodies measured with the in-house method associated with venous thrombosis and presented the strongest independent risk factor for the presence of obstetric complications among all tested antiphospholipid antibodies

  12. Cloud Absorption Radiometer Autonomous Navigation System - CANS

    Science.gov (United States)

    Kahle, Duncan; Gatebe, Charles; McCune, Bill; Hellwig, Dustan

    2013-01-01

    CAR (cloud absorption radiometer) acquires spatial reference data from host aircraft navigation systems. This poses various problems during CAR data reduction, including navigation data format, accuracy of position data, accuracy of airframe inertial data, and navigation data rate. Incorporating its own navigation system, which included GPS (Global Positioning System), roll axis inertia and rates, and three axis acceleration, CANS expedites data reduction and increases the accuracy of the CAR end data product. CANS provides a self-contained navigation system for the CAR, using inertial reference and GPS positional information. The intent of the software application was to correct the sensor with respect to aircraft roll in real time based upon inputs from a precision navigation sensor. In addition, the navigation information (including GPS position), attitude data, and sensor position details are all streamed to a remote system for recording and later analysis. CANS comprises a commercially available inertial navigation system with integral GPS capability (Attitude Heading Reference System AHRS) integrated into the CAR support structure and data system. The unit is attached to the bottom of the tripod support structure. The related GPS antenna is located on the P-3 radome immediately above the CAR. The AHRS unit provides a RS-232 data stream containing global position and inertial attitude and velocity data to the CAR, which is recorded concurrently with the CAR data. This independence from aircraft navigation input provides for position and inertial state data that accounts for very small changes in aircraft attitude and position, sensed at the CAR location as opposed to aircraft state sensors typically installed close to the aircraft center of gravity. More accurate positional data enables quicker CAR data reduction with better resolution. The CANS software operates in two modes: initialization/calibration and operational. In the initialization/calibration mode

  13. Unpredictably Stable

    DEFF Research Database (Denmark)

    Failla, Virgilio; Melillo, Francesca; Reichstein, Toke

    2014-01-01

    Is entrepreneurship a more stable career choice for high employment turnover individuals? We find that a transition to entrepreneurship induces a shift towards stayer behavior and identify job matching, job satisfaction and lock-in effects as main drivers. These findings have major implications...

  14. GPM GROUND VALIDATION CONICAL SCANNING MILLIMETER-WAVE IMAGING RADIOMETER (COSMIR) MC3E V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The GPM Ground Validation Conical Scanning Millimeter-wave Imaging Radiometer (COSMIR) MC3E dataset used the Conical Scanning Millimeter-wave Imaging Radiometer...

  15. GPM GROUND VALIDATION CONICAL SCANNING MILLIMETER-WAVE IMAGING RADIOMETER (COSMIR) GCPEX V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The GPM Ground Validation Conical Scanning Millimeter-wave Imaging Radiometer (COSMIR) GCPEx dataset used the Conical Scanning Millimeter-wave Imaging Radiometer...

  16. HURRICANE AND SEVERE STORM SENTINEL (HS3) HURRICANE IMAGING RADIOMETER (HIRAD) V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The Hurricane and Severe Storm Sentinel (HS3) Hurricane Imaging Radiometer (HIRAD) was collected by the Hurricane Imaging Radiometer (HIRAD), which was a multi-band...

  17. The design of an in-water optical radiometer

    Digital Repository Service at National Institute of Oceanography (India)

    Desa, E.S.; Desa, B.A; De

    insights into the role playEd. by absorption and scattering processes in the optical properties of water masses. In this paper, we shall describe our design approach to current development effort on a profiling optical radiometer that will measure upwelling...

  18. Calibration of the solar UV radiometers in Finland

    Energy Technology Data Exchange (ETDEWEB)

    Leszczynski, K.; Jokela, K.; Visuri, R.; Ylianttila, L. [Finnish Centre for Radiation and Nuclear Safety, Helsinki (Finland). Non-Ionizing Radiation Lab.

    1996-12-31

    In this report, the main emphasis is given to (1) the problems associated with the basic calibration of the spectroradiometer and (2) the year-to-year variability of the calibrations of the solar UV network radiometers. Also, the results from intercomparisons of the Brewer and OL 742 spectroradiometers are included

  19. High resolution soil moisture radiometer. [large space structures

    Science.gov (United States)

    Wilheit, T. T.

    1978-01-01

    An electrically scanned pushbroom phased antenna array is described for a microwave radiometer which can provide agriculturally meaningful measurements of soil moisture. The antenna size of 100 meters at 1400 MHz or 230 meters at 611 MHz requires several shuttle launches and orbital assembly. Problems inherent to the size of the structure and specific instrument problems are discussed as well as the preliminary design.

  20. Accurate antenna reflector loss measurements for radiometer calibration budget

    DEFF Research Database (Denmark)

    Skou, Niels

    1996-01-01

    Antenna reflector losses may play an important role in the calibration budget for a microwave radiometer. If the losses are small they are difficult to measure by traditional means. However, they can be assessed directly by radiometric means using the sky brightness temperature as incident...

  1. Measurement of small antenna reflector losses for radiometer calibration budget

    DEFF Research Database (Denmark)

    Skou, Niels

    1997-01-01

    Antenna reflector losses play an important role in the calibration budget for a microwave radiometer. If the losses are small, they are difficult to measure by traditional means. However, they can be assessed directly by radiometric means using the sky brightness temperature as incident radiation...

  2. Combined Radar-Radiometer Surface Soil Moisture and Roughness Estimation

    Science.gov (United States)

    Akbar, Ruzbeh; Cosh, Michael H.; O'Neill, Peggy E.; Entekhabi, Dara; Moghaddam, Mahta

    2017-01-01

    A robust physics-based combined radar-radiometer, or Active-Passive, surface soil moisture and roughness estimation methodology is presented. Soil moisture and roughness retrieval is performed via optimization, i.e., minimization, of a joint objective function which constrains similar resolution radar and radiometer observations simultaneously. A data-driven and noise-dependent regularization term has also been developed to automatically regularize and balance corresponding radar and radiometer contributions to achieve optimal soil moisture retrievals. It is shown that in order to compensate for measurement and observation noise, as well as forward model inaccuracies, in combined radar-radiometer estimation surface roughness can be considered a free parameter. Extensive Monte-Carlo numerical simulations and assessment using field data have been performed to both evaluate the algorithms performance and to demonstrate soil moisture estimation. Unbiased root mean squared errors (RMSE) range from 0.18 to 0.03 cm3cm3 for two different land cover types of corn and soybean. In summary, in the context of soil moisture retrieval, the importance of consistent forward emission and scattering development is discussed and presented.

  3. Improved noise-adding radiometer for microwave receivers

    Science.gov (United States)

    Batelaan, P. D.; Stelzried, C. T.; Goldstein, R. M.

    1973-01-01

    Use of input switch and noise reference standard is avoided by using noise-adding technique. Excess noise from solid state noise-diode is coupled into receiver through directional coupler and square-wave modulated at low rate. High sensitivity receivers for radioastronomy applications are utilized with greater confidence in stability of radiometer.

  4. Calibration OGSE for a multichannel radiometer for Mars atmosphere studies

    Science.gov (United States)

    Jiménez, J. J.; Álvarez, F. J.; Gonzalez-Guerrero, M.; Apéstigue, V.; Martin, I.; Fernández, J. M.; Fernán, A. A.; Arruego, I.

    2017-09-01

    This work describes several OGSEs (Optical Ground Support Equipment) developed by INTA (Spanish Institute of Aerospace Technology - Instituto Nacional de Técnica Aeroespacial) for the calibration and characterization of their self-manufactured multichannel radiometers (Solar Irradiance Sensors - SIS) for planetary atmospheric studies in the frame of some Martian missions at which INTA is participating.

  5. Calibration of the solar UV radiometers in Finland

    Energy Technology Data Exchange (ETDEWEB)

    Leszczynski, K; Jokela, K; Visuri, R; Ylianttila, L [Finnish Centre for Radiation and Nuclear Safety, Helsinki (Finland). Non-Ionizing Radiation Lab.

    1997-12-31

    In this report, the main emphasis is given to (1) the problems associated with the basic calibration of the spectroradiometer and (2) the year-to-year variability of the calibrations of the solar UV network radiometers. Also, the results from intercomparisons of the Brewer and OL 742 spectroradiometers are included

  6. Characterisation of optical filters for broadband UVA radiometer

    Science.gov (United States)

    Alves, Luciana C.; Coelho, Carla T.; Corrêa, Jaqueline S. P. M.; Menegotto, Thiago; Ferreira da Silva, Thiago; Aparecida de Souza, Muriel; Melo da Silva, Elisama; Simões de Lima, Maurício; Dornelles de Alvarenga, Ana Paula

    2016-07-01

    Optical filters were characterized in order to know its suitability for use in broadband UVA radiometer head for spectral irradiance measurements. The spectral transmittance, the angular dependence and the spatial uniformity of the spectral transmittance of the UVA optical filters were investigated. The temperature dependence of the transmittance was also studied.

  7. Stable particles

    International Nuclear Information System (INIS)

    Samios, N.P.

    1993-01-01

    I have been asked to review the subject of stable particles, essentially the particles that eventually comprised the meson and baryon octets. with a few more additions -- with an emphasis on the contributions made by experiments utilizing the bubble chamber technique. In this activity, much work had been done by the photographic emulsion technique and cloud chambers-exposed to cosmic rays as well as accelerator based beams. In fact, many if not most of the stable particles were found by these latter two techniques, however, the forte of the bubble chamber (coupled with the newer and more powerful accelerators) was to verify, and reinforce with large statistics, the existence of these states, to find some of the more difficult ones, mainly neutrals and further to elucidate their properties, i.e., spin, parity, lifetimes, decay parameters, etc

  8. 1999-2003 Shortwave Characterizations of Earth Radiation Budget Satellite (ERBS)/Earth Radiation Budget Experiment (ERBE) Broadband Active Cavity Radiometer Sensors

    Science.gov (United States)

    Lee, Robert B., III; Smith, George L.; Wong, Takmeng

    2008-01-01

    From October 1984 through May 2005, the NASA Earth Radiation Budget Satellite (ERBS/ )/Earth Radiation Budget Experiment (ERBE)ERBE nonscanning active cavity radiometers (ACR) were used to monitor long-term changes in the earth radiation budget components of the incoming total solar irradiance (TSI), earth-reflected TSI, and earth-emitted outgoing longwave radiation (OLR). From September1984 through September 1999, using on-board calibration systems, the ERBS/ERBE ACR sensor response changes, in gains and offsets, were determined from on-orbit calibration sources and from direct observations of the incoming TSI through calibration solar ports at measurement precision levels approaching 0.5 W/sq m , at satellite altitudes. On October 6, 1999, the onboard radiometer calibration system elevation drive failed. Thereafter, special spacecraft maneuvers were performed to observe cold space and the sun in order to define the post-September 1999 geometry of the radiometer measurements, and to determine the October 1999-September 2003 ERBS sensor response changes. Analyses of these special solar and cold space observations indicate that the radiometers were pointing approximately 16 degrees away from the spacecraft nadir and on the anti-solar side of the spacecraft. The special observations indicated that the radiometers responses were stable at precision levels approaching 0.5 W/sq m . In this paper, the measurement geometry determinations and the determinations of the radiometers gain and offset are presented, which will permit the accurate processing of the October 1999 through September 2003 ERBE data products at satellite and top-of-the-atmosphere altitudes.

  9. A New Way to Demonstrate the Radiometer as a Heat Engine

    Science.gov (United States)

    Hladkouski, V. I.; Pinchuk, A. I.

    2015-01-01

    While the radiometer is readily available as a toy, A. E. Woodruff notes that it is also a very useful tool to help us understand how to resolve certain scientific problems. Many physicists think they know how the radiometer works, but only a few actually understand it. Here we present a demonstration that shows that a radiometer can be thought of…

  10. Design of a Push-Broom Multi-Beam Radiometer for Future Ocean Observations

    DEFF Research Database (Denmark)

    Cappellin, C.; Pontoppidan, K.; Nielsen, P. H.

    2015-01-01

    The design of a push-broom multi-beam radiometer for future ocean observations is described. The radiometer provides a sensitivity one order of magnitude higher than a traditional conical scanning radiometer, and has the big advantage of being fully stationary relative to the satellite platform...

  11. Design and Development of the SMAP Microwave Radiometer Electronics

    Science.gov (United States)

    Piepmeier, Jeffrey R.; Medeiros, James J.; Horgan, Kevin A.; Brambora, Clifford K.; Estep, Robert H.

    2014-01-01

    The SMAP microwave radiometer will measure land surface brightness temperature at L-band (1413 MHz) in the presence of radio frequency interference (RFI) for soil moisture remote sensing. The radiometer design was driven by the requirements to incorporate internal calibration, to operate synchronously with the SMAP radar, and to mitigate the deleterious effects of RFI. The system design includes a highly linear super-heterodyne microwave receiver with internal reference loads and noise sources for calibration and an innovative digital signal processor and detection system. The front-end comprises a coaxial cable-based feed network, with a pair of diplexers and a coupled noise source, and radiometer front-end (RFE) box. Internal calibration is provided by reference switches and a common noise source inside the RFE. The RF back-end (RBE) downconverts the 1413 MHz channel to an intermediate frequency (IF) of 120 MHz. The IF signals are then sampled and quantized by high-speed analog-to-digital converters in the radiometer digital electronics (RDE) box. The RBE local oscillator and RDE sampling clocks are phase-locked to a common reference to ensure coherency between the signals. The RDE performs additional filtering, sub-band channelization, cross-correlation for measuring third and fourth Stokes parameters, and detection and integration of the first four raw moments of the signals. These data are packetized and sent to the ground for calibration and further processing. Here we discuss the novel features of the radiometer hardware particularly those influenced by the need to mitigate RFI.

  12. Radiometallating antibodies and biologically active peptides

    International Nuclear Information System (INIS)

    Mercer-Smith, J.A.; Roberts, J.C.; Lewis, D.; Newmyer, S.L.; Schulte, L.D.; Burns, T.P.; Mixon, P.L.; Jeffery, A.L.; Schreyer, S.A.; Cole, D.A.; Figard, S.D.; Lennon, V.A.; Hayashi, M.; Lavallee, D.K.

    1990-01-01

    We have developed methods to radiolabel large molecules, using porphyrins as bifunctional chelating agents for radiometals. The porphyrins are substituted with an N-benzyl group to activate them for radiometallation under mild reaction conditions. Porphyrins that have on functional group for covalent attachment to other molecules cannot cause crosslinking. We have examined the labeling chemistry for antibodies, and we have also developed methods to label smaller biologically active molecules, such as autoantigenic peptides. The autoantigenic peptides, fragments of the acetylcholine receptor, are under investigation for myasthenia gravis research. The methods of covalent attachment of these bifunctional chelating agents to large molecules and the radiometallation chemistry will be discussed

  13. Monoclonal antibody

    International Nuclear Information System (INIS)

    Oyamada, Hiyoshimaru

    1987-01-01

    Some aspects of monoclonal antibodies are described, centering on studies made by the author and those presented at the Second International Conference on Monoclonal Antibody Immunoconjugates for Cancer held in March this year (1987). The history of immuno-nuclear medicine and procedures for producing monoclonal antibodies are briefly outlined. Monoclonal antibodies are immunoglobulins. Here, the structure of IgG, which is used most frequently, is described. An IgG is composed of two antigen binding fragments (Fab) and one crystallizable fragment (Fc). The end portion of a Fab reacts with an antigen. One of the major applications of immuno-nuclear medicine is the diagnosis of cancer. As label nucleides, 131 I and 111 I were selected in most cases in the past while 123 I and 99m Tc are currently used more often. Advantages and disadvantages of this diagnosis method is discussed citing studies presented at the First (1986) and Second (1987) International Conference on Monoclonal Antibody Immunoconjugates for Cancer. The present status of the application of monoclonal antibodies to treatment of cancer is also described. (Nogami, K.)

  14. Stable isotopes

    International Nuclear Information System (INIS)

    Brazier, J.L.; Guinamant, J.L.

    1995-01-01

    According to the progress which has been realised in the technology of separating and measuring isotopes, the stable isotopes are used as preferable 'labelling elements' for big number of applications. The isotopic composition of natural products shows significant variations as a result of different reasons like the climate, the seasons, or their geographic origins. So, it was proved that the same product has a different isotopic composition of alimentary and agriculture products. It is also important in detecting the pharmacological and medical chemicals. This review article deals with the technology, like chromatography and spectrophotometry, adapted to this aim, and some important applications. 17 refs. 6 figs

  15. Stable Tetraquarks

    Energy Technology Data Exchange (ETDEWEB)

    Quigg, Chris [Fermilab

    2018-04-13

    For very heavy quarks, relations derived from heavy-quark symmetry imply novel narrow doubly heavy tetraquark states containing two heavy quarks and two light antiquarks. We predict that double-beauty states will be stable against strong decays, whereas the double-charm states and mixed beauty+charm states will dissociate into pairs of heavy-light mesons. Observing a new double-beauty state through its weak decays would establish the existence of tetraquarks and illuminate the role of heavy color-antitriplet diquarks as hadron constituents.

  16. Stable beams

    CERN Multimedia

    2015-01-01

    Stable beams: two simple words that carry so much meaning at CERN. When LHC page one switched from "squeeze" to "stable beams" at 10.40 a.m. on Wednesday, 3 June, it triggered scenes of jubilation in control rooms around the CERN sites, as the LHC experiments started to record physics data for the first time in 27 months. This is what CERN is here for, and it’s great to be back in business after such a long period of preparation for the next stage in the LHC adventure.   I’ve said it before, but I’ll say it again. This was a great achievement, and testimony to the hard and dedicated work of so many people in the global CERN community. I could start to list the teams that have contributed, but that would be a mistake. Instead, I’d simply like to say that an achievement as impressive as running the LHC – a machine of superlatives in every respect – takes the combined effort and enthusiasm of everyone ...

  17. Precipitation from the GPM Microwave Imager and Constellation Radiometers

    Science.gov (United States)

    Kummerow, Christian; Randel, David; Kirstetter, Pierre-Emmanuel; Kulie, Mark; Wang, Nai-Yu

    2014-05-01

    Satellite precipitation retrievals from microwave sensors are fundamentally underconstrained requiring either implicit or explicit a-priori information to constrain solutions. The radiometer algorithm designed for the GPM core and constellation satellites makes this a-priori information explicit in the form of a database of possible rain structures from the GPM core satellite and a Bayesian retrieval scheme. The a-priori database will eventually come from the GPM core satellite's combined radar/radiometer retrieval algorithm. That product is physically constrained to ensure radiometric consistency between the radars and radiometers and is thus ideally suited to create the a-priori databases for all radiometers in the GPM constellation. Until a robust product exists, however, the a-priori databases are being generated from the combination of existing sources over land and oceans. Over oceans, the Day-1 GPM radiometer algorithm uses the TRMM PR/TMI physically derived hydrometer profiles that are available from the tropics through sea surface temperatures of approximately 285K. For colder sea surface temperatures, the existing profiles are used with lower hydrometeor layers removed to correspond to colder conditions. While not ideal, the results appear to be reasonable placeholders until the full GPM database can be constructed. It is more difficult to construct physically consistent profiles over land due to ambiguities in surface emissivities as well as details of the ice scattering that dominates brightness temperature signatures over land. Over land, the a-priori databases have therefore been constructed by matching satellite overpasses to surface radar data derived from the WSR-88 network over the continental United States through the National Mosaic and Multi-Sensor QPE (NMQ) initiative. Databases are generated as a function of land type (4 categories of increasing vegetation cover as well as 4 categories of increasing snow depth), land surface temperature and

  18. Search for new and improved radiolabeling methods for monoclonal antibodies

    International Nuclear Information System (INIS)

    Hiltunen, J.V.

    1993-01-01

    In this review the selection of different radioisotopes is discussed as well as the various traditional or newer methods to introduce the radiolabel into the antibody structure. Labeling methods for radiohalogens, for technetium and rhenium isotopes, and for 3-valent cation radiometals are reviewed. Some of the newer methods offer simplified labeling procedures, but usually the new methods are more complicated than the earlier ones. However, new labeling methods are available for almost any radioelement group and they may result in better preserved original natural of the antibody and lead to better clinical results. (orig./MG)

  19. Rotating shadowband radiometer development and analysis of spectral shortwave data

    Energy Technology Data Exchange (ETDEWEB)

    Michalsky, J.; Harrison, L.; Min, Q. [State Univ. of New York, Albany, NY (United States)] [and others

    1996-04-01

    Our goals in the Atmospheric Radiation Measurement (ARM) Program are improved measurements of spectral shortwave radiation and improved techniques for the retrieval of climatologically sensitive parameters. The multifilter rotating shadowband radiometer (MFRSR) that was developed during the first years of the ARM program has become a workhorse at the Southern Great Plains (SGP) Cloud and Radiation Testbed (CART) site, and it is widely deployed in other climate programs. We have spent most of our effort this year developing techniques to retrieve column aerosol, water vapor, and ozone from direct beam spectral measurements of the MFRSR. Additionally, we have had some success in calculating shortwave surface diffuse spectral irradiance. Using the surface albedo and the global irradiance, we have calculated cloud optical depths. From cloud optical depth and liquid water measured with the microwave radiometer, we have calculated effective liquid cloud particle radii. The rest of the text will provide some detail regarding each of these efforts.

  20. Narrow Field of View Zenith Radiometer (NFOV) Handbook

    Energy Technology Data Exchange (ETDEWEB)

    Chiu, C; Marshak, A; Hodges, G; Barnard, JC; Schmelzer, J

    2008-11-01

    The two-channel narrow field-of-view radiometer (NFOV2) is a ground-based radiometer that looks straight up and measures radiance directly above the instrument at wavelengths of 673 and 870 nm. The field-of-view of the instrument is 1.2 degrees, and the sampling time resolution is one second. Measurements of the NFOV2 have been used to retrieve optical properties for overhead clouds that range from patchy to overcast. With a one-second sampling rate of the NFOV2, faster than almost any other ARM Climate Research Facility (ACRF) instrument, we are able, for the first time, to capture changes in cloud optical properties at the natural time scale of cloud evolution.

  1. Effect of a spacer moiety on radiometal labelled Neurotensin derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Mascarin, A.; Valverde, I.E.; Mindt, T.L. [Univ. of Basel Hospital (Switzerland). Div. of Radiopharmaceutical Chemistry

    2013-07-01

    The binding sequence of the regulatory peptide Neurotensin, NT(8-13), represents a promising tumour-specific vector for the development of radiopeptides useful in nuclear oncology for the diagnosis (imaging) and therapy of cancer. A number of radiometal-labelled NT(8-13) derivatives have been reported, however, the effect of the spacer which connects the vector with the radiometal complex has yet not been investigated systematically. Because a spacer moiety can influence potentially important biological characteristics of radiopeptides, we synthesized three [DOTA({sup 177}Lu)]-X-NT(8-13) derivatives and evaluated the effect of a spacer (X) on the physico-chemical properties of the conjugate including lipophilicity, stability, and in vitro receptor affinity and cell internalization. (orig.)

  2. The Along Track Scanning Radiometer (ATSR) for ERS1

    Science.gov (United States)

    Delderfield, J.; Llewellyn-Jones, D. T.; Bernard, R.; de Javel, Y.; Williamson, E. J.

    1986-01-01

    The ATSR is an infrared imaging radiometer which has been selected to fly aboard the ESA Remote Sensing Satellite No. 1 (ERS1) with the specific objective of accurately determining global Sea Surface Temperature (SST). Novel features, including the technique of 'along track' scanning, a closed Stirling cycle cooler, and the precision on-board blackbodies are described. Instrument subsystems are identified and their design trade-offs discussed.

  3. Evaluation of a novel enzyme-linked immunosorbent assay for detection of antibodies against Salmonella, employing a stable coating of lipopolysaccharide-derived antigens covalently attached to polystyrene microwells

    DEFF Research Database (Denmark)

    Wiuff, C.; Jauho, E. S.; Stryhn, H.

    2000-01-01

    to microtiter polystyrene plates by ultraviolet irradiation. Both polysaccharide antigens could be coupled simultaneously to the same microtiter plate. The coated surface was used in indirect ELISA for the determination of serum antibodies from pigs infected with bacteria of the two Salmonella groups and from......Polysaccharides derived from Salmonella typhimurium lipopolysaccharide (LPS) representing the O-antigen factors 1, 4, 5, and 12 and the O-antigen factors 6 and 7 from Salmonella choleraesuis LPS were derivatized with the photoreactive compound anthraquinone and subsequently covalently coupled...

  4. Optimum Image Formation for Spaceborne Microwave Radiometer Products.

    Science.gov (United States)

    Long, David G; Brodzik, Mary J

    2016-05-01

    This paper considers some of the issues of radiometer brightness image formation and reconstruction for use in the NASA-sponsored Calibrated Passive Microwave Daily Equal-Area Scalable Earth Grid 2.0 Brightness Temperature Earth System Data Record project, which generates a multisensor multidecadal time series of high-resolution radiometer products designed to support climate studies. Two primary reconstruction algorithms are considered: the Backus-Gilbert approach and the radiometer form of the scatterometer image reconstruction (SIR) algorithm. These are compared with the conventional drop-in-the-bucket (DIB) gridded image formation approach. Tradeoff study results for the various algorithm options are presented to select optimum values for the grid resolution, the number of SIR iterations, and the BG gamma parameter. We find that although both approaches are effective in improving the spatial resolution of the surface brightness temperature estimates compared to DIB, SIR requires significantly less computation. The sensitivity of the reconstruction to the accuracy of the measurement spatial response function (MRF) is explored. The partial reconstruction of the methods can tolerate errors in the description of the sensor measurement response function, which simplifies the processing of historic sensor data for which the MRF is not known as well as modern sensors. Simulation tradeoff results are confirmed using actual data.

  5. A new real time infrared background discrimination radiometer (BDR)

    International Nuclear Information System (INIS)

    Kopolovich, Z.; Cabib, D.; Buckwald, R.A.

    1989-01-01

    This paper reports on a new radiometer (BDR) that has been developed, which discriminates small differences between an object and its surrounding background, and is able to measure an object's changing contrast when the contrast of a moving object is to be measured against a changing background. The difference in radiant emittance of a small object against its background or of two objects with respect to each other and this difference is small compared to the emittance itself. Practical examples of such measurements are contrast measurements of airplanes and missiles in flight, contrast measurements of small, weak objects on a warm background and uniformity measurements of radiant emittance from an object's surface. Previous instruments were unable to make such measurements since the process of contrast measurement with a fixed field of view radiometer is too slow for implementation on flying objects; detection of a small difference between two large DC signals is impossible in a traditional fixed field of view radiometer when the instrument itself is saturated

  6. A horizontal vane radiometer: Experiment, theory, and simulation

    Energy Technology Data Exchange (ETDEWEB)

    Wolfe, David; Larraza, Andres, E-mail: larraza@nps.edu [Department of Physics, Naval Postgraduate School, Monterey, California 93940 (United States); Garcia, Alejandro [Department of Physics and Astronomy, San Jose State University, San Jose, California 95152 (United States)

    2016-03-15

    The existence of two motive forces on a Crookes radiometer has complicated the investigation of either force independently. The thermal creep shear force in particular has been subject to differing interpretations of the direction in which it acts and its order of magnitude. In this article, we provide a horizontal vane radiometer design which isolates the thermal creep shear force. The horizontal vane radiometer is explored through experiment, kinetic theory, and the Direct Simulation Monte Carlo (DSMC) method. The qualitative agreement between the three methods of investigation is good except for a dependence of the force on the width of the vane even when the temperature gradient is narrower than the vane which is present in the DSMC method results but not in the theory. The experimental results qualitatively resemble the theory in this regard. The quantitative agreement between the three methods of investigation is better than an order of magnitude in the cases examined. The theory is closer to the experimental values for narrow vanes and the simulations are closer to the experimental values for the wide vanes. We find that the thermal creep force acts from the hot side to the cold side of the vane. We also find the peak in the radiometer’s angular speed as a function of pressure is explained as much by the behavior of the drag force as by the behavior of the thermal creep force.

  7. A horizontal vane radiometer: Experiment, theory, and simulation

    International Nuclear Information System (INIS)

    Wolfe, David; Larraza, Andres; Garcia, Alejandro

    2016-01-01

    The existence of two motive forces on a Crookes radiometer has complicated the investigation of either force independently. The thermal creep shear force in particular has been subject to differing interpretations of the direction in which it acts and its order of magnitude. In this article, we provide a horizontal vane radiometer design which isolates the thermal creep shear force. The horizontal vane radiometer is explored through experiment, kinetic theory, and the Direct Simulation Monte Carlo (DSMC) method. The qualitative agreement between the three methods of investigation is good except for a dependence of the force on the width of the vane even when the temperature gradient is narrower than the vane which is present in the DSMC method results but not in the theory. The experimental results qualitatively resemble the theory in this regard. The quantitative agreement between the three methods of investigation is better than an order of magnitude in the cases examined. The theory is closer to the experimental values for narrow vanes and the simulations are closer to the experimental values for the wide vanes. We find that the thermal creep force acts from the hot side to the cold side of the vane. We also find the peak in the radiometer’s angular speed as a function of pressure is explained as much by the behavior of the drag force as by the behavior of the thermal creep force.

  8. Manual of program operation for data analysis from radiometer system

    International Nuclear Information System (INIS)

    Silva Mello, L.A.R. da; Migliora, C.G.S.

    1987-12-01

    This manual describes how to use the software to retrieve and analyse data from radiometer systems and raingauges used in the 12 GHz PROPAGATION MEASUREMENTS/CANADA - TELEBRAS COOPERATION PROGRAM. The data retrieval and analisys is being carried out by CETUC, as part of the activities of the project Simulacao de Enlaces Satelite (SES). The software for these tasks has been supplied by the Canadian Research Centre (CRC), together with the measurement equipment. The two following sections describe the use of the data retrieval routines and the data analysis routines of program ATTEN. Also, a quick reference guide for commands that can be used when a microcomputer is local or remotely connected to a radiometer indoor unit is included as a last section. A more detailed description of these commands, their objectives and cautions that should de taken when using them can be found in the manual ''12 GHz Propagation Measurements System - Volume 1 - Dual Slope Radiometer and Data Aquisition System'', supplied by Diversitel Communications Inc. (author) [pt

  9. Catalytic Antibodies

    Indian Academy of Sciences (India)

    biological processes and is intended to catalyze a reaction for which no real enzyme is ... the reaction. In order to enhance the rates of chemical reactions, enzymes, ..... of such antibodies has already been exploited in the production of a biosensor. ..... tant to the pharmaceutical and fine chemical industries for the synthesis ...

  10. The multi-filter rotating shadowband radiometer (MFRSR) - precision infrared radiometer (PIR) platform in Fairbanks: Scientific objectives

    Energy Technology Data Exchange (ETDEWEB)

    Stamnes, K.; Leontieva, E. [Univ. of Alaska, Fairbanks (United States)

    1996-04-01

    The multi-filter rotating shadowband radiometer (MFRSR) and precision infrared radiometer (PIR) have been employed at the Geophysical Institute in Fairbanks to check their performance under arctic conditions. Drawing on the experience of the previous measurements in the Arctic, the PIR was equipped with a ventilator to prevent frost and moisture build-up. We adopted the Solar Infrared Observing Sytem (SIROS) concept from the Southern Great Plains Cloud and Radiation Testbed (CART) to allow implementation of the same data processing software for a set of radiation and meteorological instruments. To validate the level of performance of the whole SIROS prior to its incorporation into the North Slope of Alaska (NSA) Cloud and Radiation Testbed Site instrumental suite for flux radiatin measurements, the comparison between measurements and model predictions will be undertaken to assess the MFRSR-PIR Arctic data quality.

  11. Radiolabeled monoclonal antibodies for imaging and therapy: Potential, problems, and prospects: Scientific highlights

    International Nuclear Information System (INIS)

    Srivastava, S.C.; Buraggi, G.L.

    1986-01-01

    This meeting focused on areas of research on radiolabeled monoclonal antibodies. Topics covered included the production, purification, and fragmentation of monoclonal antibodies and immunochemistry of hybridomas; the production and the chemistry of radionuclides; the radiohalogenation and radiometal labeling techniques; the in-vivo pharmacokinetics of radiolabeled antibodies; the considerations of immunoreactivity of radiolabeled preparations; the instrumentation and imaging techniques as applied to radioimmunodetection; the radiation dosimetry in diagnostic and therapeutic use of labeled antibodies; the radioimmunoscintigraphy and radioimmunotherapy studies; and perspectives and directions for future research. Tutorial as well as scientific lectures describing the latest research data on the above topics were presented. Three workshop panels were convened on ''Methods for Determining Immunoreactivity of Radiolabeled Monoclonal Antibodies - Problems and Pitfalls,'' Radiobiological and Dosimetric Considerations for Immunotherapy with Labeled Antibodies,'' and ''The Human Anti-Mouse Antibody Response in Patients.''

  12. Antiparietal cell antibody test

    Science.gov (United States)

    APCA; Anti-gastric parietal cell antibody; Atrophic gastritis - anti-gastric parietal cell antibody; Gastric ulcer - anti-gastric parietal cell antibody; Pernicious anemia - anti-gastric parietal cell antibody; ...

  13. Boreal Inundation Mapping with SMAP Radiometer Data for Methane Studies

    Science.gov (United States)

    Kim, Seungbum; Brisco, Brian; Poncos, Valentin

    2017-04-01

    Inundation and consequent anoxic condition induce methane release, which is one of the most potent greenhouse gases. Boreal regions contain large amounts of organic carbon, which is a potentially major methane emission source under climatic warming conditions. Boreal wetlands in particular are one of the largest sources of uncertainties in global methane budget. Wetland spatial extent together with the gas release rate remains highly unknown. Characterization of the existing inundation database is poor, because of the inundation under clouds and dense vegetation. In this work, the inundation extent is derived using brightness temperature data acquired by the L-band Soil Moisture Active Passive (SMAP) satellite, which offers the L-band capabilities to penetrate clouds and vegetation at 3-day revisit. The fidelity of the SMAP watermask is assessed as a first step in this investigation by comparing with the following data sets: 3-m resolution maps derived using Radarsat synthetic aperture radar (SAR) data in northern Canada and multi-sensor climatology over Siberia. Because Radarsat coverages are limited despite its high spatial resolution, at the time and location where Radarsats are not available, we also compare with 3-km resolution SMAP SAR data that are concurrent with the SMAP radiometer data globally until July 2015. Inundation extents were derived with Radarsat, SMAP SAR, and SMAP radiometer over the 60 km x 60km area at Peace Athabasca Delta (PAD), Canada on 6 days in spring and summer 2015. The SMAP SAR results match the locations of Radarsat waterbodies. However, the SMAP SAR underestimates the water extent, mainly over mixed pixels that have subpixel land presence. The threshold value (-3 dB) applied to the SMAP SAR was determined previously over the global domain. The threshold is dependent on the type of local landcover within a mixed pixel. Further analysis is needed to locally optimize the threshold. The SMAP radiometer water fraction over Peace

  14. Radiolabeled monoclonal antibodies: a review

    International Nuclear Information System (INIS)

    Toledo e Souza, I.T. de; Okada, H.

    1990-05-01

    Since the description by Kohler and Milstein 1975 of their technique for producing monoclonal antibodies of predefined specificity, it has become a mainstay in most laboratories that utilize immunochemical techniques to study problems in basic, applied or clinical research. Paradoxically, the very success of monoclonal antibodies has generated a literature which is now so vast and scattered that it has become difficult to obtain a perspective. This brief review represents the distillation of many publications relating to the production and use of monoclonaal antibodies as radiopharmaceuticals. Significant advances were made possible in the last few years by combined developments in the fields of tumor-associated antigens and of monoclonal antibodies. In fact monoclonal antibodies against some well defined tumor-associated antigens, has led to significantly greater practical possibilities for producing highly specific radiolabeled antibodies as radiopharmaceuticals for diagnosis and therapy of human tumors. One of the main requirements of this methodology is the availability of stable radiopharmaceutical reagents which after labeling in vivo injection retain the capacity of specific interaction with the defined antigen and their molecular integrity. Since injection into human is the objetive of this kind of study all the specifications of radiopharmaceutical have to be fulfilled e.g. sterility, apirogenicity and absence of toxicity. (author) [pt

  15. Radiometer Calibration and Characterization (RCC) User's Manual: Windows Version 4.0

    Energy Technology Data Exchange (ETDEWEB)

    Andreas, Afshin M. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Wilcox, Stephen M. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-02-29

    The Radiometer Calibration and Characterization (RCC) software is a data acquisition and data archival system for performing Broadband Outdoor Radiometer Calibrations (BORCAL). RCC provides a unique method of calibrating broadband atmospheric longwave and solar shortwave radiometers using techniques that reduce measurement uncertainty and better characterize a radiometer's response profile. The RCC software automatically monitors and controls many of the components that contribute to uncertainty in an instrument's responsivity. This is a user's manual and guide to the RCC software.

  16. Application of microwave radiometers for wetlands and estuaries monitoring

    International Nuclear Information System (INIS)

    Shutko, A.; Haldin, A.; Novichikhin, E.

    1997-01-01

    This paper presents the examples of experimental data obtained with airborne microwave radiometers used for monitoring of wetlands and estuaries located in coastal environments. The international team of researchers has successfully worked in Russia, Ukraine and USA. The data presented relate to a period of time between 1990 and 1995. They have been collected in Odessa Region, Black Sea coast, Ukraine, in Regions of Pittsville and Winfield, Maryland, USA, and in Region of St. Marks, Florida, USA. The parameters discussed are a soil moisture, depth to a shallow water table, vegetation index, salinity of water surface

  17. Antibody Engineering and Therapeutics

    Science.gov (United States)

    Almagro, Juan Carlos; Gilliland, Gary L; Breden, Felix; Scott, Jamie K; Sok, Devin; Pauthner, Matthias; Reichert, Janice M; Helguera, Gustavo; Andrabi, Raiees; Mabry, Robert; Bléry, Mathieu; Voss, James E; Laurén, Juha; Abuqayyas, Lubna; Barghorn, Stefan; Ben-Jacob, Eshel; Crowe, James E; Huston, James S; Johnston, Stephen Albert; Krauland, Eric; Lund-Johansen, Fridtjof; Marasco, Wayne A; Parren, Paul WHI; Xu, Kai Y

    2014-01-01

    The 24th Antibody Engineering & Therapeutics meeting brought together a broad range of participants who were updated on the latest advances in antibody research and development. Organized by IBC Life Sciences, the gathering is the annual meeting of The Antibody Society, which serves as the scientific sponsor. Preconference workshops on 3D modeling and delineation of clonal lineages were featured, and the conference included sessions on a wide variety of topics relevant to researchers, including systems biology; antibody deep sequencing and repertoires; the effects of antibody gene variation and usage on antibody response; directed evolution; knowledge-based design; antibodies in a complex environment; polyreactive antibodies and polyspecificity; the interface between antibody therapy and cellular immunity in cancer; antibodies in cardiometabolic medicine; antibody pharmacokinetics, distribution and off-target toxicity; optimizing antibody formats for immunotherapy; polyclonals, oligoclonals and bispecifics; antibody discovery platforms; and antibody-drug conjugates. PMID:24589717

  18. Stable coordination of the inhibitory Ca2+ ion at MIDAS in integrin CD11b/CD18 by an antibody-derived ligand aspartate: Implications for integrin regulation and structure-based drug design

    Science.gov (United States)

    Mahalingam, Bhuvaneshwari; Ajroud, Kaouther; Alonso, Jose Luis; Anand, Saurabh; Adair, Brian; Horenstein, Alberto L; Malavasi, Fabio; Xiong, Jian-Ping; Arnaout, M. Amin

    2011-01-01

    A central feature of integrin interaction with physiologic ligands is the monodentate binding of a ligand carboxylate to a Mg2+ ion hexacoordinated at the metal-ion-dependent-adhesion site (MIDAS) in the integrin A-domain. This interaction stabilizes the A-domain in the high-affinity state, which is distinguished from the default low-affinity state by tertiary changes in the domain that culminate in cell adhesion. Small molecule ligand-mimetic integrin antagonists act as partial agonists, eliciting similar activating conformational changes in the A-domain, which has contributed to paradoxical adhesion and increased patient mortality in large clinical trials. As with other ligand-mimetic integrin antagonists, the function-blocking monoclonal antibody (mAb) 107 binds MIDAS of integrin CD11b/CD18 A-domain (CD11bA), but in contrast, it favors the inhibitory Ca2+ ion over Mg2+ at MIDAS. We determined the crystal structures of the Fab fragment of mAb 107 complexed to the low- and high-affinity states of CD11bA. Favored binding of Ca2+ at MIDAS is caused by the unusual symmetric bidentate ligation of a Fab-derived ligand Asp to a heptacoordinated MIDAS Ca2+. Binding of Fab 107 to CD11bA did not trigger the activating tertiary changes in the domain or in the full-length integrin. These data show that denticity of the ligand Asp/Glu can modify divalent cation selectivity at MIDAS and hence integrin function. Stabilizing the Ca2+ ion at MIDAS by bidentate ligation to a ligand Asp/Glu may provide one approach for designing pure integrin antagonists. PMID:22095715

  19. Validation of ocean color sensors using a profiling hyperspectral radiometer

    Science.gov (United States)

    Ondrusek, M. E.; Stengel, E.; Rella, M. A.; Goode, W.; Ladner, S.; Feinholz, M.

    2014-05-01

    Validation measurements of satellite ocean color sensors require in situ measurements that are accurate, repeatable and traceable enough to distinguish variability between in situ measurements and variability in the signal being observed on orbit. The utility of using a Satlantic Profiler II equipped with HyperOCR radiometers (Hyperpro) for validating ocean color sensors is tested by assessing the stability of the calibration coefficients and by comparing Hyperpro in situ measurements to other instruments and between different Hyperpros in a variety of water types. Calibration and characterization of the NOAA Satlantic Hyperpro instrument is described and concurrent measurements of water-leaving radiances conducted during cruises are presented between this profiling instrument and other profiling, above-water and moored instruments. The moored optical instruments are the US operated Marine Optical BuoY (MOBY) and the French operated Boussole Buoy. In addition, Satlantic processing versions are described in terms of accuracy and consistency. A new multi-cast approach is compared to the most commonly used single cast method. Analysis comparisons are conducted in turbid and blue water conditions. Examples of validation matchups with VIIRS ocean color data are presented. With careful data collection and analysis, the Satlantic Hyperpro profiling radiometer has proven to be a reliable and consistent tool for satellite ocean color validation.

  20. Modeling the frequency response of microwave radiometers with QUCS

    International Nuclear Information System (INIS)

    Zonca, A; Williams, B; Rubin, I; Meinhold, P; Lubin, P; Roucaries, B; D'Arcangelo, O; Franceschet, C; Mennella, A; Bersanelli, M; Jahn, S

    2010-01-01

    Characterization of the frequency response of coherent radiometric receivers is a key element in estimating the flux of astrophysical emissions, since the measured signal depends on the convolution of the source spectral emission with the instrument band shape. Laboratory Radio Frequency (RF) measurements of the instrument bandpass often require complex test setups and are subject to a number of systematic effects driven by thermal issues and impedance matching, particularly if cryogenic operation is involved. In this paper we present an approach to modeling radiometers bandpasses by integrating simulations and RF measurements of individual components. This method is based on QUCS (Quasi Universal Circuit Simulator), an open-source circuit simulator, which gives the flexibility of choosing among the available devices, implementing new analytical software models or using measured S-parameters. Therefore an independent estimate of the instrument bandpass is achieved using standard individual component measurements and validated analytical simulations. In order to automate the process of preparing input data, running simulations and exporting results we developed the Python package python-qucs and released it under GNU Public License. We discuss, as working cases, bandpass response modeling of the COFE and Planck Low Frequency Instrument (LFI) radiometers and compare results obtained with QUCS and with a commercial circuit simulator software. The main purpose of bandpass modeling in COFE is to optimize component matching, while in LFI they represent the best estimation of frequency response, since end-to-end measurements were strongly affected by systematic effects.

  1. Four-channel temperature and humidity microwave scanning radiometer

    Science.gov (United States)

    Xu, Pei-Yuan

    1994-06-01

    A compact four-channel microwave scanning radiometer for tropospheric remote sensing is being developed. A pair of 53.85 and 56.02 GHz and a pair of 23.87 and 31.65 GHz are adopted as temperature and humidity channels' frequencies respectively. For each pair of frequencies it has an offset reflector antenna and a Dicke-switching receiver. The pair of receivers is assembled in an enclosure, which is mounted on the rotating table of an azimuth mounting and the pair of antennas is connected with the rotating table of an azimuth mounting in the opposite side by a pair of elevation arms. Each antenna is composed of a 90 degree off-set paraboloid and a conical corrugated horn. Each antenna patterrn of four channels has nearly same HPBW, low side lobes, and low VSWR. The dual band humidity receiver is a time sharing type with 0.2K sensitivity at 1-sec integration time. The dual band temperature receiver is a band sharing type with 0.2K sensitivity at 10-sec integration time. The radiometer and observation are controlled by a single chip microcomputer to realize the unattended operation.

  2. Infrared fibers for radiometer thermometry in hypothermia and hyperthermia treatment

    International Nuclear Information System (INIS)

    Katzir, A.; Bowman, H.F.; Asfour, Y.; Zur, A.; Valeri, C.R.

    1989-01-01

    Hypothermia is a condition which results from prolonged exposure to a cold environment. Rapid and efficient heating is needed to rewarm the patient from 32-35 degrees C to normal body temperature. Hyperthermia in cancer treatment involves heating malignant tumors to 42.5-43.0 degrees C for an extended period (e.g., 30 min) in an attempt to obtain remission. Microwave or radio frequency heating is often used for rewarming in hypothermia or for temperature elevation in hyperthermia treatment. One severe problem with such heating is the accurate measurement and control of temperature in the presence of a strong electromagnetic field. For this purpose, we have developed a fiberoptic radiometer system which is based on a nonmetallic, infrared fiber probe, which can operate either in contact or noncontact mode. In preliminary investigations, the radiometer worked well in a strong microwave or radiofrequency field, with an accuracy of +/- 0.5 degrees C. This fiberoptic thermometer was used to control the surface temperature of objects within +/- 2 degrees C

  3. Physical, biological, and chemical data from radiometer, profiling reflectance radiometer, and CTD casts in a world-wide distribution as part of the SeaWiFS/SIMBIOS project from 13 September 1981 to 16 December 1999 (NODC Accession 0000632)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Physical, biological, and chemical data were collected using radiometer, profiling reflectance radiometer, and CTD casts in a world-wide distribution from 13...

  4. Compact Front-end Prototype for Next Generation RFI-rejecting Polarimetric L-band Radiometer

    DEFF Research Database (Denmark)

    Jensen, Brian Sveistrup; Søbjærg, Sten Schmidl; Skou, Niels

    2009-01-01

    Realizing the need for lower noise figure and smaller physical size in todays higly sensitive radiometers, this paper presents a new compact analog front-end (AFE) for use with the existing L-band (1400-1427 MHz) radiometer designed and operated by the Technical University of Denmark. Using subha...

  5. A simple method to minimize orientation effects in a profiling radiometer

    Digital Repository Service at National Institute of Oceanography (India)

    Suresh, T.; Talaulikar, M.; Desa, E.; Matondkar, S.G.P.; SrinivasaKumar, T.; Lotlikar, A.

    -fall radiometer is found to be a better option for measuring underwater light parameters as it avoids the effects of ship shadow and is easy to operate, the measurements demand profiling the radiometer vertical in water with minimum tilt. Here we present...

  6. Spaceborne L-band Radiometers: Push-broom or Synthetic Aperture?

    DEFF Research Database (Denmark)

    Skou, Niels

    2004-01-01

    L-band radiometers can measure ocean salinity and soil moisture from space. A synthetic aperture radiometer system, SMOS, is under development by ESA for launch in 2007. A real aperture push-broom system, Aquarius, has been approved by NASA for launch in 2008. Pros et cons of the two fundamentally...

  7. Soil Moisture ActivePassive (SMAP) L-Band Microwave Radiometer Post-Launch Calibration

    Science.gov (United States)

    Peng, Jinzheng; Piepmeier, Jeffrey R.; Misra, Sidharth; Dinnat, Emmanuel P.; Hudson, Derek; Le Vine, David M.; De Amici, Giovanni; Mohammed, Priscilla N.; Yueh, Simon H.; Meissner, Thomas

    2016-01-01

    The SMAP microwave radiometer is a fully-polarimetric L-band radiometer flown on the SMAP satellite in a 6 AM/ 6 PM sun-synchronous orbit at 685 km altitude. Since April, 2015, the radiometer is under calibration and validation to assess the quality of the radiometer L1B data product. Calibration methods including the SMAP L1B TA2TB (from Antenna Temperature (TA) to the Earth’s surface Brightness Temperature (TB)) algorithm and TA forward models are outlined, and validation approaches to calibration stability/quality are described in this paper including future work. Results show that the current radiometer L1B data satisfies its requirements.

  8. ATSR - The Along Track Scanning Radiometer For ERS-1

    Science.gov (United States)

    Llewellyn-Jones, David T.; Mutlow, C. T.

    1990-04-01

    The ATSR instrument is an advanced imaging radiometer designed to measure global sea surface temperature to an accuracy of the order of 0.3C from the ESA's ERS-1 satellite, due to be launched in late 1990. The instrument is designed to achieve a very precise correction for atmospheric effects through the use of carefully selected spectral bands, and a new "along-track" scanning technique. This involves viewing the same geophysical scene at two different angles, hence using two different atmospheric paths, so that the difference in radiative signal from the two scenes is due only to atmospheric effects, which can then be quantitatively estimated. ATSR is also a high performance radiometer, and embodies two important technological features; the first of these is the use of closed-cycle coolers, especially developed for space applications, and which were used to cool the sensitive infrared detectors. The radiometer also incorporates two purpose-designed on-board blackbody calibration targets which will also be described in detail. These two features enable the instrument to meet the stringent requirements of sensitivity and absolute radiometric accuracy demanded by this application. ATSR also incorporates a passive nadir-viewing two-channel microwave sounder. Measurements from this instrument will enable total atmospheric water vapour to be inferred, which will not only lead to improved SST retrievals, but will also considerably improve the atmospheric range correction required by the ERS-1 radar altimeter. ATSR is provided by a consortium of research institutes including the University of Oxford, Department of Atmospheric Oceanic and Planetary Physics, who are primarily responsible for scientific calibration of the instrument; University College London's Mullard Space Science Laboratory, who are responsible for the development of the blackbodies; the UK Meteorological Office, whose contributions include the focal plane assembly; the French laboratory CRPE, who are

  9. Characterization of NPP Visible/Infrared Imager Radiometer Suite (VIIRS) Reflective Solar Bands Dual Gain Anomaly

    Science.gov (United States)

    Lee, Shihyan; McIntire, Jeff; Oudari, Hassan

    2012-01-01

    The Visible/Infrared Imager Radiometer Suite (VIIRS) contains six dual gain bands in the reflective solar spectrum. The dual gain bands are designed to switch gain mode at pre-defined thresholds to achieve high resolution at low radiances while maintaining the required dynamic range for science. During pre-launch testing, an anomaly in the electronic response before transitioning from high to low gain was discovered and characterized. On-orbit, the anomaly was confirmed using MODIS data collected during Simultaneous Nadir Overpasses (SNOs). The analysis of the Earth scene data shows that dual gain anomaly can be determined at the orbital basis. To characterize the dual gain anomaly, the anomaly region and electronic offsets were tracked per week during the first 8 month of VIIRS operation. The temporal analysis shows the anomaly region can drift 20 DN and is impacted by detectors DC Restore. The estimated anomaly flagging regions cover 2.5 % of the high gain dynamic range and are consistent with prelaunch and on-orbit LUT. The prelaunch results had a smaller anomaly range (30-50 DN) and are likely the results of more stable electronics from the shorter data collection time. Finally, this study suggests future calibration efforts to focus on the anomaly's impact on science products and possible correction method to reduce uncertainties.

  10. Total ozone retrieval from satellite multichannel filter radiometer measurements

    International Nuclear Information System (INIS)

    Lovill, J.E.; Sullivan, T.J.; Weichel, R.L.; Ellis, J.S.; Huebel, J.G.; Korver, J.; Weidhaas, P.P.; Phelps, F.A.

    1978-01-01

    A total ozone retrieval model has been developed to process radiance data gathered by a satellite-mounted multichannel filter radiometer (MFR). Extensive effort went into theoretical radiative transfer modeling, a retrieval scheme was developed, and the technique was applied to the MFR radiance measurements. The high quality of the total ozone retrieval results was determined through comparisons with Dobson measurements. Included in the report are global total ozone maps for 20 days between May 12 and July 5, 1977. A comparison of MFR results for 13 days in June 1977 with Dobson spectrophotometer measurements of ozone for the same period showed good agreement: there was a root-mean-square difference of 6.2% (equivalent to 20.2 m.atm.cm). The estimated global total ozone value for June 1977 (296 m.atm.cm) was in good agreement with satellite backscatter ultraviolet data for June 1970 (304 m.atm.cm) and June 1971

  11. Optical power calibrator based on a stabilized green He-Ne laser and a cryogenic absolute radiometer

    International Nuclear Information System (INIS)

    Varpula, T.; Seppa, H.; Saari, J.M.

    1989-01-01

    This paper describes an optical power calibrator whose overall calibration uncertainty is less than 10 -4 for an optical power of 0.13 mW. The laser light source of the system operates at a wavelength of 543.5 nm, being close to the wavelength at which the candela is defined, 555 nm. A stable optical power is achieved by stabilizing the intensity and the frequency of a green He-Ne laser. The optical power is detected by a cryogenic absolute radiometer based on the principle of electrical substitution radiometry. It can be employed to measure optical power up to 0.5 mW in the visible and near infrared region

  12. Stable Isotope Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Tissue samples (skin, bone, blood, muscle) are analyzed for stable carbon, stable nitrogen, and stable sulfur analysis. Many samples are used in their entirety for...

  13. Icecube: Spaceflight Validation of an 874-GHz Submillimeter Wave Radiometer for Ice Cloud Remote Sensing

    Science.gov (United States)

    Wu, D. L.; Esper, J.; Ehsan, N.; Piepmeier, J. R.; Racette, P.

    2014-12-01

    Ice clouds play a key role in the Earth's radiation budget, mostly through their strong regulation of infrared radiation exchange. Submillimeter wave remote sensing offers a unique capability to improve cloud ice measurements from space. At 874 GHz cloud scattering produces a larger brightness temperature depression from cirrus than lower frequencies, which can be used to retrieve vertically-integrated cloud ice water path (IWP) and ice particle size. The objective of the IceCube project is to retire risks of 874-GHz receiver technology by raising its TRL from 5 to 7. The project will demonstrate, on a 3-U CubeSat in a low Earth orbit (LEO) environment, the 874-GHz receiver system with noise equivalent differential temperature (NEDT) of ~0.2 K for 1-second integration and calibration error of 2.0 K or less as measured from deep-space observations. The Goddard Space Flight Center (GSFC) is partnering with Virginia Diodes, Inc (VDI) to qualify commercially available 874-GHz receiver technology for spaceflight, and demonstrate the radiometer performance. The instrument (submm-wave cloud radiometer, or SCR), along with the CubeSat system developed and integrated by GSFC, will be ready for launch in two years. The instrument subsystem includes a reflector antenna, sub-millimeter wave mixer, frequency multipliers and stable local oscillator, an intermediate frequency (IF) circuit with noise injection, and data-power boards. The mixer and frequency multipliers are procured from VDI with GSFC insight into fabrication and testing processes to ensure scalability to spaceflight beyond TRL 7. The remaining components are a combination of GSFC-designed and commercial off-the-shelf (COTS) at TRLs of 5 or higher. The spacecraft system is specified by GSFC and comprises COTS components including three-axis stabilizer and sun sensor, GPS receiver, deployable solar arrays, UHF radio, and 2 GB of on-board storage. The spacecraft and instrument are integrated and flight qualified

  14. The Impact of Indoor and Outdoor Radiometer Calibration on Solar Measurements: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Habte, Aron; Sengupta, Manajit; Andreas, Afshin; Reda, Ibrahim; Robinson, Justin

    2016-07-01

    Accurate solar radiation data sets are critical to reducing the expenses associated with mitigating performance risk for solar energy conversion systems, and they help utility planners and grid system operators understand the impacts of solar resource variability. The accuracy of solar radiation measured by radiometers depends on the instrument performance specification, installation method, calibration procedure, measurement conditions, maintenance practices, location, and environmental conditions. This study addresses the effect of calibration methodologies and the resulting calibration responsivities provided by radiometric calibration service providers such as the National Renewable Energy Laboratory (NREL) and manufacturers of radiometers. Some of these radiometers are calibrated indoors, and some are calibrated outdoors. To establish or understand the differences in calibration methodology, we processed and analyzed field-measured data from these radiometers. This study investigates calibration responsivities provided by NREL's broadband outdoor radiometer calibration (BORCAL) and a few prominent manufacturers. The reference radiometer calibrations are traceable to the World Radiometric Reference. These different methods of calibration demonstrated 1% to 2% differences in solar irradiance measurement. Analyzing these values will ultimately assist in determining the uncertainties of the radiometer data and will assist in developing consensus on a standard for calibration.

  15. Evaluation of Radiometers Deployed at the National Renewable Energy Laboratory's Solar Radiation Research Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Habte, Aron; Wilcox, Stephen; Stoffel, Thomas

    2015-12-23

    This study analyzes the performance of various commercially available radiometers used for measuring global horizontal irradiances and direct normal irradiances. These include pyranometers, pyrheliometers, rotating shadowband radiometers, and a pyranometer with fixed internal shading and are all deployed at the National Renewable Energy Laboratory's Solar Radiation Research Laboratory. Data from 32 global horizontal irradiance and 19 direct normal irradiance radiometers are presented. The radiometers in this study were deployed for one year (from April 1, 2011, through March 31, 2012) and compared to measurements from radiometers with the lowest values of estimated measurement uncertainties for producing reference global horizontal irradiances and direct normal irradiances.

  16. Application of RUB-01P beta radiometer to control contamination of milk and dairy produce

    International Nuclear Information System (INIS)

    Bachurin, A.V.; Donskaya, G.A.; Koroleva, M.S.; Titov, S.K.

    1990-01-01

    RUB-01P beta-radiometer to control radioactive contamination of milk and dairy produce characterized by a number of advantages as compared to RKB-4-1eM manufactured earlier is described. Device is designed using a new element base, simgle-action, characterized by increased reliability, can operate on-line with ELEKTRONIKA MK-64 programmed microcalculater. Radiometer output is printed out to a void operator errors and to record measurement results. Radiometer main error is maximum 50 %. Data on device sensitivity at measurements using BDZhB-05P, BDZhB-06P1, BDZhB-06P detection units are given

  17. Processor breadboard for on-board RFI detection and mitigation in MetOp-SG radiometers

    DEFF Research Database (Denmark)

    Skou, Niels; Kristensen, Steen S.; Kovanen, Arhippa

    2015-01-01

    Radio Frequency Interference (RFI) is an increasing threat to proper operation of space-borne Earth viewing microwave radiometer systems. There is a steady growth in active services, and tougher requirements to sensitivity and fidelity of future radiometer systems. Thus it has been decided...... that the next generation MetOp satellites must include some kind of RFI detection and mitigation system at Ku band. This paper describes a breadboard processor that detects and mitigates RFI on-board the satellite. Thus cleaned data can be generated in real time, and following suitable integration, downloaded...... to ground at the modest data rate usually associated with radiometer systems....

  18. Stable convergence and stable limit theorems

    CERN Document Server

    Häusler, Erich

    2015-01-01

    The authors present a concise but complete exposition of the mathematical theory of stable convergence and give various applications in different areas of probability theory and mathematical statistics to illustrate the usefulness of this concept. Stable convergence holds in many limit theorems of probability theory and statistics – such as the classical central limit theorem – which are usually formulated in terms of convergence in distribution. Originated by Alfred Rényi, the notion of stable convergence is stronger than the classical weak convergence of probability measures. A variety of methods is described which can be used to establish this stronger stable convergence in many limit theorems which were originally formulated only in terms of weak convergence. Naturally, these stronger limit theorems have new and stronger consequences which should not be missed by neglecting the notion of stable convergence. The presentation will be accessible to researchers and advanced students at the master's level...

  19. A broad set of different llama antibodies specific for a 16 kDa heat shock protein of Mycobacterium tuberculosis

    NARCIS (Netherlands)

    Trilling, Anke K.; de Ronde, Hans; Noteboom, Linda; van Houwelingen, Adèle; Roelse, Margriet; Srivastava, Saurabh K.; Haasnoot, Willem; Jongsma, Maarten A.; Kolk, Arend; Zuilhof, Han; Beekwilder, Jules

    2011-01-01

    Recombinant antibodies are powerful tools in engineering of novel diagnostics. Due to the small size and stable nature of llama antibody domains selected antibodies can serve as a detection reagent in multiplexed and sensitive assays for M. tuberculosis. Antibodies for Mycobacterium tuberculosis (M.

  20. An optical scanning subsystem for a UAS-enabled hyperspectral radiometer

    Data.gov (United States)

    National Aeronautics and Space Administration — Hyperspectral radiometers will be integrated with an optical scanning subsystem to measure remote sensing reflectance spectra over the ocean.  The entire scanning...

  1. GPM GROUND VALIDATION ADVANCED MICROWAVE RADIOMETER RAIN IDENTIFICATION (ADMIRARI) GCPEX V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The GPM Ground Validation Advanced Microwave Radiometer Rain Identification (ADMIRARI) GCPEx dataset measures brightness temperature at three frequencies (10.7, 21.0...

  2. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Snow Cover Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of snow cover from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument...

  3. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Smoothed Normalized Difference Vegetation Index (NDVI) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Visible Infrared Imaging Radiometer Suite (VIIRS) Smoothed Normalized Difference Vegetation Index (NDVI) from NDE is a weekly product derived from the VIIRS...

  4. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Aerosol Detection Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of suspended matter from the Visible Infrared Imaging Radiometer Suite (VIIRS)...

  5. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Sensor Data Record (SDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Sensor Data Records (SDRs), or Level 1b data, from the Visible Infrared Imaging Radiometer Suite (VIIRS) are the calibrated and geolocated radiance and reflectance...

  6. Low level beta-activity radiometer with compensation of the background

    Energy Technology Data Exchange (ETDEWEB)

    Vankov, I [and others

    1996-12-31

    New type of the low level beta-activity scintillation detector system is developed. The procedure of finding the beta activity and the operation of the recording unit of the radiometer are considered. 3 refs.; 5 figs.

  7. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Mask Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains a high quality Environmental Data Record (EDR) of cloud masks from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument onboard...

  8. Effect of vegetation on soil moisture sensing observed from orbiting microwave radiometers

    International Nuclear Information System (INIS)

    Wang, J.R.

    1985-01-01

    The microwave radiometric measurements made by the Skylab 1.4 GHz radiometer and by the 6.6 GHz and 10.7 GHz channels of the Nimbus-7 Scanning Multichannel Microwave Radiometer were analyzed to study the large-area soil moisture variations of land surfaces. Two regions in Texas, one with sparse and the other with dense vegetation covers, were selected for the study. The results gave a confirmation of the vegetation effect observed by ground-level microwave radiometers. Based on the statistics of the satellite data, it was possible to estimate surface soil moisture in about five different levels from dry to wet conditions with a 1.4 GHz radiometer, provided that the biomass of the vegetation cover could be independently measured. At frequencies greater than about 6.6 GHz, the radiometric measurements showed little sensitivity to moisture variation for vegetation-covered soils. The effects of polarization in microwave emission were studied also. (author)

  9. Nimbus-2 Level 2 Medium Resolution Infrared Radiometer (MRIR) V001

    Data.gov (United States)

    National Aeronautics and Space Administration — The Nimbus II Medium Resolution Infrared Radiometer (MRIR) was designed to measure electromagnetic radiation emitted and reflected from the earth and its atmosphere...

  10. Calibration of IR test chambers with the missile defense transfer radiometer

    Science.gov (United States)

    Kaplan, Simon G.; Woods, Solomon I.; Carter, Adriaan C.; Jung, Timothy M.

    2013-05-01

    The Missile Defense Transfer Radiometer (MDXR) is designed to calibrate infrared collimated and flood sources over the fW/cm2 to W/cm2 power range from 3 μm to 28μ m in wavelength. The MDXR operates in three different modes: as a filter radiometer, a Fourier-transform spectrometer (FTS)-based spectroradiometer, and as an absolute cryogenic radiometer (ACR). Since 2010, the MDXR has made measurements of the collimated infrared irradiance at the output port of seven different infrared test chambers at several facilities. We present a selection of results from these calibration efforts compared to signal predictions from the respective chamber models for the three different MDXR calibration modes. We also compare the results to previous measurements made of the same chambers with a legacy transfer radiometer, the NIST BXR. In general, the results are found to agree within their combined uncertainties, with the MDXR having 30 % lower uncertainty and greater spectral coverage.

  11. Scanning Multichannel Microwave Radiometer (SMMR) Monthly Mean Atmospheric Liquid Water (ALW) By Prabhakara

    Data.gov (United States)

    National Aeronautics and Space Administration — SMMR_ALW_PRABHAKARA data are Special Multichannel Microwave Radiometer (SMMR) Monthly Mean Atmospheric Liquid Water (ALW) data by Prabhakara.The Prabhakara Scanning...

  12. The Aquarius Ocean Salinity Mission High Stability L-band Radiometer

    Science.gov (United States)

    Pellerano, Fernando A.; Piepmeier, Jeffrey; Triesky, Michael; Horgan, Kevin; Forgione, Joshua; Caldwell, James; Wilson, William J.; Yueh, Simon; Spencer, Michael; McWatters, Dalia; hide

    2006-01-01

    The NASA Earth Science System Pathfinder (ESSP) mission Aquarius, will measure global ocean surface salinity with approx.120 km spatial resolution every 7-days with an average monthly salinity accuracy of 0.2 psu (parts per thousand). This requires an L-band low-noise radiometer with the long-term calibration stability of less than or equal to 0.15 K over 7 days. The instrument utilizes a push-broom configuration which makes it impractical to use a traditional warm load and cold plate in front of the feedhorns. Therefore, to achieve the necessary performance Aquarius utilizes a Dicke radiometer with noise injection to perform a warm - hot calibration. The radiometer sequence between antenna, Dicke load, and noise diode has been optimized to maximize antenna observations and therefore minimize NEDT. This is possible due the ability to thermally control the radiometer electronics and front-end components to 0.1 Crms over 7 days.

  13. Evaluating Solar Resource Data Obtained from Multiple Radiometers Deployed at the National Renewable Energy Laboratory: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Habte, A.; Sengupta, M.; Andreas, A.; Wilcox, S.; Stoffel, T.

    2014-09-01

    Solar radiation resource measurements from radiometers are used to predict and evaluate the performance of photovoltaic and concentrating solar power systems, validate satellite-based models for estimating solar resources, and advance research in solar forecasting and climate change. This study analyzes the performance of various commercially available radiometers used for measuring global horizontal irradiances (GHI) and direct normal irradiances (DNI). These include pyranometers, pyrheliometers, rotating shadowband irradiometers, and a pyranometer with a shading ring deployed at the National Renewable Energy Laboratory's Solar Radiation Research Laboratory (SRRL). The radiometers in this study were deployed for one year (from April 1, 2011, through March 31, 2012) and compared to measurements from radiometers with the lowest values of estimated measurement uncertainties for producing reference GHI and DNI.

  14. Next-Generation Thermal Infrared Multi-Body Radiometer Experiment (TIMBRE)

    Science.gov (United States)

    Kenyon, M.; Mariani, G.; Johnson, B.; Brageot, E.; Hayne, P.

    2016-10-01

    We have developed an instrument concept called TIMBRE which belongs to the important class of instruments called thermal imaging radiometers (TIRs). TIMBRE is the next-generation TIR with unparalleled performance compared to the state-of-the-art.

  15. The Planck-LFI Radiometer Electronics Box Assembly

    International Nuclear Information System (INIS)

    Herreros, J M; Gomez, M F; Rebolo, R; Chulani, H; Rubino-Martin, J A; Hildebrandt, S R; Bersanelli, M; Franceschet, C; Butler, R C; Miccolis, M; Pena, A; Pereira, M; Torrero, F; Lopez, M; Alcala, C

    2009-01-01

    The Radiometer Electronics Box Assembly (REBA) is the control and data processing on board computer of the Low Frequency Instrument (LFI) of the Planck mission (ESA). The REBA was designed and built incorporating state of the art processors, communication interfaces and real time operating system software in order to meet the scientific performance of the LFI. We present a technical summary of the REBA, including a physical, functional, electrical, mechanical and thermal description. Aspects of the design and development, the assembly, the integration and the verification of the equipment are provided. A brief description of the LFI on board software is given including the Low-Level Software and the main functionalities and architecture of the Application Software. The compressor module, which has been developed as an independent product, later integrated in the application, is also described in this paper. Two identical engineering models EM and AVM, the engineering qualification model EQM, the flight model FM and flight spare have been manufactured and tested. Low-level and Application software have been developed. Verification activities demonstrated that the REBA hardware and software fulfil all the specifications and perform as required for flight operation.

  16. The Planck-LFI Radiometer Electronics Box Assembly

    Science.gov (United States)

    Herreros, J. M.; Gómez, M. F.; Rebolo, R.; Chulani, H.; Rubiño-Martin, J. A.; Hildebrandt, S. R.; Bersanelli, M.; Butler, R. C.; Miccolis, M.; Peña, A.; Pereira, M.; Torrero, F.; Franceschet, C.; López, M.; Alcalá, C.

    2009-12-01

    The Radiometer Electronics Box Assembly (REBA) is the control and data processing on board computer of the Low Frequency Instrument (LFI) of the Planck mission (ESA). The REBA was designed and built incorporating state of the art processors, communication interfaces and real time operating system software in order to meet the scientific performance of the LFI. We present a technical summary of the REBA, including a physical, functional, electrical, mechanical and thermal description. Aspects of the design and development, the assembly, the integration and the verification of the equipment are provided. A brief description of the LFI on board software is given including the Low-Level Software and the main functionalities and architecture of the Application Software. The compressor module, which has been developed as an independent product, later integrated in the application, is also described in this paper. Two identical engineering models EM and AVM, the engineering qualification model EQM, the flight model FM and flight spare have been manufactured and tested. Low-level and Application software have been developed. Verification activities demonstrated that the REBA hardware and software fulfil all the specifications and perform as required for flight operation.

  17. CIRiS: Compact Infrared Radiometer in Space

    Science.gov (United States)

    Osterman, D. P.; Collins, S.; Ferguson, J.; Good, W.; Kampe, T.; Rohrschneider, R.; Warden, R.

    2016-09-01

    The Compact Infrared Radiometer in Space (CIRiS) is a thermal infrared radiometric imaging instrument under development by Ball Aerospace for a Low Earth Orbit mission on a CubeSat spacecraft. Funded by the NASA Earth Science Technology Office's In-Space Validation of Earth Science Technology (InVEST) program, the mission objective is technology demonstration for improved on-orbit radiometric calibration. The CIRiS calibration approach uses a scene select mirror to direct three calibration views to the focal plane array and to transfer the resulting calibrated response to earth images. The views to deep space and two blackbody sources, including one at a selectable temperature, provide multiple options for calibration optimization. Two new technologies, carbon nanotube blackbody sources and microbolometer focal plane arrays with reduced pixel sizes, enable improved radiometric performance within the constrained 6U CubeSat volume. The CIRiS instrument's modular design facilitates subsystem modifications as required by future mission requirements. CubeSat constellations of CIRiS and derivative instruments offer an affordable approach to achieving revisit times as short as one day for diverse applications including water resource and drought management, cloud, aerosol, and dust studies, and land use and vegetation monitoring. Launch is planned for 2018.

  18. The Planck-LFI Radiometer Electronics Box Assembly

    Energy Technology Data Exchange (ETDEWEB)

    Herreros, J M; Gomez, M F; Rebolo, R; Chulani, H; Rubino-Martin, J A; Hildebrandt, S R [Instituto de Astrofisica de Canarias (IAC), 38200 La Laguna, Tenerife (Spain); Bersanelli, M; Franceschet, C [Universita di Milano, Dipartamento di Fisica, Via G. Celoria 16, 20133 Milano (Italy); Butler, R C [INAF-IASF Bologna, Via P. Gobetti 101, 40129 Bologna (Italy); Miccolis, M [Thales Alenia Space Italia S.p.A., IUEL - Scientific Instruments, S.S. Padana Superiore 290, 20090 Vimodrone (Italy); Pena, A; Pereira, M; Torrero, F; Lopez, M; Alcala, C, E-mail: rrl@iac.e [EADS Astrium CRISA, C/Torres Quevedo, 9, 28760 Tres Cantos (Spain)

    2009-12-15

    The Radiometer Electronics Box Assembly (REBA) is the control and data processing on board computer of the Low Frequency Instrument (LFI) of the Planck mission (ESA). The REBA was designed and built incorporating state of the art processors, communication interfaces and real time operating system software in order to meet the scientific performance of the LFI. We present a technical summary of the REBA, including a physical, functional, electrical, mechanical and thermal description. Aspects of the design and development, the assembly, the integration and the verification of the equipment are provided. A brief description of the LFI on board software is given including the Low-Level Software and the main functionalities and architecture of the Application Software. The compressor module, which has been developed as an independent product, later integrated in the application, is also described in this paper. Two identical engineering models EM and AVM, the engineering qualification model EQM, the flight model FM and flight spare have been manufactured and tested. Low-level and Application software have been developed. Verification activities demonstrated that the REBA hardware and software fulfil all the specifications and perform as required for flight operation.

  19. Regolith Formation Rates and Evolution from the Diviner Lunar Radiometer

    Science.gov (United States)

    Hayne, P. O.; Ghent, R. R.; Bandfield, J. L.; Vasavada, A. R.; Williams, J. P.; Siegler, M. A.; Lucey, P. G.; Greenhagen, B. T.; Elder, C. M.; Paige, D. A.

    2015-12-01

    Fragmentation and overturn of lunar surface materials produces a layer of regolith, which increases in thickness through time. Experiments on the lunar surface during the Apollo era, combined with remote sensing, found that the upper 10's of cm of regolith exhibit a rapid increase in density and thermal conductivity with depth. This is interpreted to be the signature of impact gardening, which operates most rapidly in the uppermost layers. Gravity data from the GRAIL mission showed that impacts have also extensively fractured the deeper crust. The breakdown and mixing of crustal materials is therefore a central process to lunar evolution and must be understood in order to interpret compositional information from remote sensing and sample analysis. Recently, thermal infrared data from the Lunar Reconnaissance Orbiter (LRO) Diviner radiometer were used to provide the first remote observational constraints on the rate of ejecta breakdown around craters L., Campbell, B. A., Allen, C. C., Carter, L. M., & Paige, D. A. (2014). Constraints on the recent rate of lunar ejecta breakdown and implications for crater ages. Geology, 42(12), 1059-1062.

  20. Daily quality assurance software for a satellite radiometer system

    Science.gov (United States)

    Keegstra, P. B.; Smoot, G. F.; Bennett, C. L.; Aymon, J.; Backus, C.; Deamici, G.; Hinshaw, G.; Jackson, P. D.; Kogut, A.; Lineweaver, C.

    1992-01-01

    Six Differential Microwave Radiometers (DMR) on COBE (Cosmic Background Explorer) measure the large-angular-scale isotropy of the cosmic microwave background (CMB) at 31.5, 53, and 90 GHz. Quality assurance software analyzes the daily telemetry from the spacecraft to ensure that the instrument is operating correctly and that the data are not corrupted. Quality assurance for DMR poses challenging requirements. The data are differential, so a single bad point can affect a large region of the sky, yet the CMB isotropy requires lengthy integration times (greater than 1 year) to limit potential CMB anisotropies. Celestial sources (with the exception of the moon) are not, in general, visible in the raw differential data. A 'quicklook' software system was developed that, in addition to basic plotting and limit-checking, implements a collection of data tests as well as long-term trending. Some of the key capabilities include the following: (1) stability analysis showing how well the data RMS averages down with increased data; (2) a Fourier analysis and autocorrelation routine to plot the power spectrum and confirm the presence of the 3 mK 'cosmic' dipole signal; (3) binning of the data against basic spacecraft quantities such as orbit angle; (4) long-term trending; and (5) dipole fits to confirm the spacecraft attitude azimuth angle.

  1. A cryogenic electrical substitution radiometer for hard X-rays

    International Nuclear Information System (INIS)

    Gerlach, M.; Krumrey, M.; Cibik, L.; Mueller, P.; Ulm, G.

    2007-01-01

    Cryogenic electrical substitution radiometers (ESR) are well established in radiometry to determine radiant power with low uncertainties from the infrared to the soft X-ray region. The absorbers are made of copper to achieve a small time constant. At higher photon energies, the use of copper prevents the operation of ESR due to increasing transmittance. A new absorber design for hard X-rays has been developed at the laboratory of the Physikalisch-Technische Bundesanstalt (PTB) at BESSY II. In the first place, extensive simulations were performed for a variety of materials and absorber geometries using the Monte Carlo simulation code Geant4. The accuracy of the simulations was verified comparing them to scattering experiments performed at a 7 T wavelength shifter beamline at BESSY II. It was shown that Geant4 describes the photo-effect, including fluorescence as well as Compton- and Rayleigh scattering, with high accuracy. The simulations and experiments resulted in an absorber with a gold base 500 μm in thickness, inclined by 30 deg., and a cylindrical shell made of copper 80 μm in thickness to reduce losses caused mainly by fluorescence. The absorber was manufactured at PTB by means of electroforming and was implemented into an existing ESR. Monochromatized synchrotron radiation of high spectral purity was used to calibrate silicon photodiodes against the ESR for photon energies up to 60 keV with relative uncertainties below 1%

  2. A cryogenic electrical substitution radiometer for hard X-rays

    Energy Technology Data Exchange (ETDEWEB)

    Gerlach, M. [Physikalisch-Technische Bundesanstalt, Abbestr. 2-12, 10587 Berlin (Germany)], E-mail: Martin.Gerlach@ptb.de; Krumrey, M.; Cibik, L.; Mueller, P.; Ulm, G. [Physikalisch-Technische Bundesanstalt, Abbestr. 2-12, 10587 Berlin (Germany)

    2007-09-21

    Cryogenic electrical substitution radiometers (ESR) are well established in radiometry to determine radiant power with low uncertainties from the infrared to the soft X-ray region. The absorbers are made of copper to achieve a small time constant. At higher photon energies, the use of copper prevents the operation of ESR due to increasing transmittance. A new absorber design for hard X-rays has been developed at the laboratory of the Physikalisch-Technische Bundesanstalt (PTB) at BESSY II. In the first place, extensive simulations were performed for a variety of materials and absorber geometries using the Monte Carlo simulation code Geant4. The accuracy of the simulations was verified comparing them to scattering experiments performed at a 7 T wavelength shifter beamline at BESSY II. It was shown that Geant4 describes the photo-effect, including fluorescence as well as Compton- and Rayleigh scattering, with high accuracy. The simulations and experiments resulted in an absorber with a gold base 500 {mu}m in thickness, inclined by 30 deg., and a cylindrical shell made of copper 80 {mu}m in thickness to reduce losses caused mainly by fluorescence. The absorber was manufactured at PTB by means of electroforming and was implemented into an existing ESR. Monochromatized synchrotron radiation of high spectral purity was used to calibrate silicon photodiodes against the ESR for photon energies up to 60 keV with relative uncertainties below 1%.

  3. A cryogenic electrical substitution radiometer for hard X-rays

    Science.gov (United States)

    Gerlach, M.; Krumrey, M.; Cibik, L.; Müller, P.; Ulm, G.

    2007-09-01

    Cryogenic electrical substitution radiometers (ESR) are well established in radiometry to determine radiant power with low uncertainties from the infrared to the soft X-ray region. The absorbers are made of copper to achieve a small time constant. At higher photon energies, the use of copper prevents the operation of ESR due to increasing transmittance. A new absorber design for hard X-rays has been developed at the laboratory of the Physikalisch-Technische Bundesanstalt (PTB) at BESSY II. In the first place, extensive simulations were performed for a variety of materials and absorber geometries using the Monte Carlo simulation code Geant4. The accuracy of the simulations was verified comparing them to scattering experiments performed at a 7 T wavelength shifter beamline at BESSY II. It was shown that Geant4 describes the photo-effect, including fluorescence as well as Compton- and Rayleigh scattering, with high accuracy. The simulations and experiments resulted in an absorber with a gold base 500 μm in thickness, inclined by 30°, and a cylindrical shell made of copper 80 μm in thickness to reduce losses caused mainly by fluorescence. The absorber was manufactured at PTB by means of electroforming and was implemented into an existing ESR. Monochromatized synchrotron radiation of high spectral purity was used to calibrate silicon photodiodes against the ESR for photon energies up to 60 keV with relative uncertainties below 1%.

  4. Determination of total ozone from DMSP multichannel filter radiometer measurements

    International Nuclear Information System (INIS)

    Luther, F.M.; Weichel, R.L.

    1992-01-01

    The multichannel filter radiometer (MFR) infrared sensor was first flown in 1977 on a Defense Meteorological Satellite Program (DMSP) Block 5D series satellite operated by the US Air Force. The first four satellites in this series carried MFR sensors from which total atmospheric column ozone amounts may be derived. The MFR sensor was the first cross-track scanning sensor capable of measuring ozone. MFR sensor infrared measurements are taken day and night. The satellites are in polar sun-synchronous orbits providing daily global coverage. The series of four sensors spans a data period of nearly three years. The MFR sensor measures infrared radiances for 16 channels. Total ozone amounts are determined from sets of radiance measurements using an empirical relationship that is developed using linear regression analysis. Total ozone is modeled as a linear combination of terms involving functions of the MFR radiances for four channels (1, 3, 7 and 16) and the secant of the zenith angle. The MFR scans side to side in discrete steps of 40. The MFR sensor takes infrared radiance measurements at 25 cross-track scanning locations every 32 seconds. The instrument could take a theoretical maximum of 67,500 measurements per day, although typically 35,000 - 45,000 measurements are taken per day

  5. PERBANDINGAN PENGUKURAN RADIOMETER DAN RADIOSONDE PADA MUSIM HUJAN DI DRAMAGA BOGOR

    OpenAIRE

    Athoillah, Ibnu; Dewi, Saraswati; Renggono, Findy

    2016-01-01

    IntisariBalai Besar Teknologi Modifikasi Cuaca (BB-TMC) BPPT bekerjasama dengan Badan Meteorologi Klimatologi dan Geofisika (BMKG) melakukan kegiatan Intensive Observation Period (IOP) selama puncak musim hujan pada tanggal 18 Januari - 16 Februari 2016 di wilayah Jabodetabek. Salah satu peralatan yang digunakan untuk observasi adalah Radiometer dan Radiosonde. Pada penelitian ini akan difokuskan bagaimana perbandingan hasil dari pengukuran Radiometer dan Radiosonde selama kegiatan IOP teruta...

  6. Characterization of antibody-chelator conjugates: Determination of chelator content by terbium fluorescence titration

    Energy Technology Data Exchange (ETDEWEB)

    Brandt, K.D.; Schnobrich, K.E.; Johnson, D.K. (Abbott Laboratories, Department 90M, Abbott Park, IL (United States))

    1991-01-01

    Fluorescence titrations were performed by adding varying mole ratios of terbium(III) to antibody conjugates formed by benzyl isothiocyanate derivatives of three different polyaminopolycarboxylate chelators (NTA, EDTA, and DTPA) and the results compared to values for average chelator content obtained by cobalt-57 binding assays. For two different murine monoclonal antibodies, the average chelator content obtained by terbium fluorescence titration correlated closely with that measured by the cobalt-57 binding assay. It is concluded that lanthanide fluorescence titrations provide a useful alternative to radiometal binding assays for the determination of chelator content in protein-chelator conjugates.

  7. Characterization of antibody-chelator conjugates: Determination of chelator content by terbium fluorescence titration

    International Nuclear Information System (INIS)

    Brandt, K.D.; Schnobrich, K.E.; Johnson, D.K.

    1991-01-01

    Fluorescence titrations were performed by adding varying mole ratios of terbium(III) to antibody conjugates formed by benzyl isothiocyanate derivatives of three different polyaminopolycarboxylate chelators (NTA, EDTA, and DTPA) and the results compared to values for average chelator content obtained by cobalt-57 binding assays. For two different murine monoclonal antibodies, the average chelator content obtained by terbium fluorescence titration correlated closely with that measured by the cobalt-57 binding assay. It is concluded that lanthanide fluorescence titrations provide a useful alternative to radiometal binding assays for the determination of chelator content in protein-chelator conjugates

  8. CAROLS: A New Airborne L-Band Radiometer for Ocean Surface and Land Observations

    Directory of Open Access Journals (Sweden)

    Ernesto Lopez-Baeza

    2011-01-01

    Full Text Available The “Cooperative Airborne Radiometer for Ocean and Land Studies” (CAROLS L-Band radiometer was designed and built as a copy of the EMIRAD II radiometer constructed by the Technical University of Denmark team. It is a fully polarimetric and direct sampling correlation radiometer. It is installed on board a dedicated French ATR42 research aircraft, in conjunction with other airborne instruments (C-Band scatterometer—STORM, the GOLD-RTR GPS system, the infrared CIMEL radiometer and a visible wavelength camera. Following initial laboratory qualifications, three airborne campaigns involving 21 flights were carried out over South West France, the Valencia site and the Bay of Biscay (Atlantic Ocean in 2007, 2008 and 2009, in coordination with in situ field campaigns. In order to validate the CAROLS data, various aircraft flight patterns and maneuvers were implemented, including straight horizontal flights, circular flights, wing and nose wags over the ocean. Analysis of the first two campaigns in 2007 and 2008 leads us to improve the CAROLS radiometer regarding isolation between channels and filter bandwidth. After implementation of these improvements, results show that the instrument is conforming to specification and is a useful tool for Soil Moisture and Ocean Salinity (SMOS satellite validation as well as for specific studies on surface soil moisture or ocean salinity.

  9. Correlation function analysis of the COBE differential microwave radiometer sky maps

    Energy Technology Data Exchange (ETDEWEB)

    Lineweaver, Charles Howe [Univ. of California, Berkeley, CA (United States). Space Sciences Lab.

    1994-08-01

    The Differential Microwave Radiometer (DMR) aboard the COBE satellite has detected anisotropies in the cosmic microwave background (CMB) radiation. A two-point correlation function analysis which helped lead to this discovery is presented in detail. The results of a correlation function analysis of the two year DMR data set is presented. The first and second year data sets are compared and found to be reasonably consistent. The positive correlation for separation angles less than ~20° is robust to Galactic latitude cuts and is very stable from year to year. The Galactic latitude cut independence of the correlation function is strong evidence that the signal is not Galactic in origin. The statistical significance of the structure seen in the correlation function of the first, second and two year maps is respectively > 9σ, > 10σ and > 18σ above the noise. The noise in the DMR sky maps is correlated at a low level. The structure of the pixel temperature covariance matrix is given. The noise covariance matrix of a DMR sky map is diagonal to an accuracy of better than 1%. For a given sky pixel, the dominant noise covariance occurs with the ring of pixels at an angular separation of 60° due to the 60° separation of the DMR horns. The mean covariance of 60° is 0.45%$+0.18\\atop{-0.14}$ of the mean variance. The noise properties of the DMR maps are thus well approximated by the noise properties of maps made by a single-beam experiment. Previously published DMR results are not significantly affected by correlated noise.

  10. stableGP

    Data.gov (United States)

    National Aeronautics and Space Administration — The code in the stableGP package implements Gaussian process calculations using efficient and numerically stable algorithms. Description of the algorithms is in the...

  11. Angina Pectoris (Stable Angina)

    Science.gov (United States)

    ... Peripheral Artery Disease Venous Thromboembolism Aortic Aneurysm More Angina Pectoris (Stable Angina) Updated:Aug 21,2017 You may have heard the term “angina pectoris” or “stable angina” in your doctor’s office, ...

  12. Calibration OGSEs for multichannel radiometers for Mars atmosphere studies

    Science.gov (United States)

    Jiménez, J. J.; J Álvarez, F.; Gonzalez-Guerrero, M.; Apéstigue, V.; Martín, I.; Fernández, J. M.; Fernán, A. A.; Arruego, I.

    2018-06-01

    This work describes several Optical Ground Support Equipment (OGSEs) developed by INTA (Spanish Institute of Aerospace Technology—Instituto Nacional de Técnica Aeroespacial) for the calibration and characterization of their self-manufactured multichannel radiometers (solar irradiance sensors—SIS) developed for working on the surface of Mars and studying the atmosphere of that planet. Nowadays, INTA is developing two SIS for the ESA ExoMars 2020 and for the JPL/NASA Mars 2020 missions. These calibration OGSEs have been improved since the first model in 2011 developed for Mars MetNet Precursor mission. This work describes the currently used OGSE. Calibration tests provide an objective evidence of the SIS performance, allowing the conversion of the electrical sensor output into accurate physical measurements (irradiance) with uncertainty bounds. Calibration results of the SIS on board of the Dust characterisation, Risk assessment, and Environment Analyzer on the Martian Surface (DREAMS) on board the ExoMars 2016 Schiaparelli module (EDM—entry and descent module) are also presented, as well as their error propagation. Theoretical precision and accuracy of the instrument are determined by these results. Two types of OGSE are used as a function of the pursued aim: calibration OGSEs and Optical Fast Verification (OFV) GSE. Calibration OGSEs consist of three setups which characterize with the highest possible accuracy, the responsivity, the angular response and the thermal behavior; OFV OGSE verify that the performance of the sensor is close to nominal after every environmental and qualification test. Results show that the accuracy of the calibrated sensors is a function of the accuracy of the optical detectors and of the light conditions. For normal direct incidence and diffuse light, the accuracy is in the same order of uncertainty as that of the reference cell used for fixing the irradiance, which is about 1%.

  13. Pushbroom microwave radiometer results from HAPEX-MOBILHY

    International Nuclear Information System (INIS)

    Nichols, W.E.; Cuenca, R.H.; Schmugge, T.J.; Wang, J.R.

    1993-01-01

    The NASA C-130 remote sensing aircraft was in Toulouse, France from 25 May through 4 July 1986, for participation in the HAPEX-MOBILHY program. Spectral and radiometric data were collected by C-130 borne sensors in the visible, infrared, and microwave wavelengths. These data provided information on the spatial and temporal variations of surface parameters such as vegetation indices, surface temperature, and surface soil moisture. The Pushbroom Microwave Radiometer (PBMR) was used to collect passive microwave brightness temperature data. This four-beam sensor operates at the 21-cm wavelength, providing cross-track coverage approximately 1.2 times the aircraft altitude. Observed brightness temperatures for the period were high, ranging from above 240 K about 290 K. Brightness temperature images appeared to correspond well to spatial and temporal soil moisture variation. Previous research has demonstrated that an approximately linear relationship exists between the surface emissivity and surface soil moisture. For these data, however, regression analysis did not indicate a strong linear relationship (r 2 = 0.32 and r 2 = 0.42 respectively) because of the limited range of soil moisture conditions encountered and the small number of ground measurements. When results from wetter soil conditions encountered in another experiment were included, the regression improved dramatically. Based on similar research with the PBMR and an understanding of the ground data collection program, this result was examined to produce recommendations for improvements to future passive microwave research and data collection programs. Examples of surface soil moisture maps generated with PBMR data are presented which appear to be representative of the actual soil moisture conditions

  14. Calibration OGSEs for multichannel radiometers for Mars atmosphere studies

    Science.gov (United States)

    Jiménez, J. J.; J Álvarez, F.; Gonzalez-Guerrero, M.; Apéstigue, V.; Martín, I.; Fernández, J. M.; Fernán, A. A.; Arruego, I.

    2018-02-01

    This work describes several Optical Ground Support Equipment (OGSEs) developed by INTA (Spanish Institute of Aerospace Technology—Instituto Nacional de Técnica Aeroespacial) for the calibration and characterization of their self-manufactured multichannel radiometers (solar irradiance sensors—SIS) developed for working on the surface of Mars and studying the atmosphere of that planet. Nowadays, INTA is developing two SIS for the ESA ExoMars 2020 and for the JPL/NASA Mars 2020 missions. These calibration OGSEs have been improved since the first model in 2011 developed for Mars MetNet Precursor mission. This work describes the currently used OGSE. Calibration tests provide an objective evidence of the SIS performance, allowing the conversion of the electrical sensor output into accurate physical measurements (irradiance) with uncertainty bounds. Calibration results of the SIS on board of the Dust characterisation, Risk assessment, and Environment Analyzer on the Martian Surface (DREAMS) on board the ExoMars 2016 Schiaparelli module (EDM—entry and descent module) are also presented, as well as their error propagation. Theoretical precision and accuracy of the instrument are determined by these results. Two types of OGSE are used as a function of the pursued aim: calibration OGSEs and Optical Fast Verification (OFV) GSE. Calibration OGSEs consist of three setups which characterize with the highest possible accuracy, the responsivity, the angular response and the thermal behavior; OFV OGSE verify that the performance of the sensor is close to nominal after every environmental and qualification test. Results show that the accuracy of the calibrated sensors is a function of the accuracy of the optical detectors and of the light conditions. For normal direct incidence and diffuse light, the accuracy is in the same order of uncertainty as that of the reference cell used for fixing the irradiance, which is about 1%.

  15. Lyme disease antibody

    Science.gov (United States)

    ... JavaScript. The Lyme disease blood test looks for antibodies in the blood to the bacteria that causes ... needed. A laboratory specialist looks for Lyme disease antibodies in the blood sample using the ELISA test . ...

  16. Antinuclear antibody panel

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003535.htm Antinuclear antibody panel To use the sharing features on this page, please enable JavaScript. The antinuclear antibody panel is a blood test that looks at ...

  17. Acetylcholine receptor antibody

    Science.gov (United States)

    ... medlineplus.gov/ency/article/003576.htm Acetylcholine receptor antibody To use the sharing features on this page, please enable JavaScript. Acetylcholine receptor antibody is a protein found in the blood of ...

  18. Nuclear medicine: Monoclonal antibodies

    International Nuclear Information System (INIS)

    Endo, K.; Sakahara, H.; Koizumi, M.; Kawamura, Y.; Torizuka, K.; Yokoyama, A.

    1986-01-01

    Antitumor monoclonal antibody was successfully labeled with Tc-99m by using dithiosemicarbazone (DTS) as a bifunctional chelating agent. In the first step, DTS was coupled to antibody without loss of immunoreactivity; the compound then efficiently formed a neutral 1:1 chelate with pentavalent or tetravalent Tc-99m. Imaging with Tc-99m-labeled monoclonal antibody to human osteosarcoma (OST-7) clearly displayed a small tumor in nude mice at 6 and 24 hours after intravenous administration. The tumor-to-blood ratio of the Tc-99m-labeled monoclonal antibody was higher than that of a radioiodinated antibody and similar to that of an In-111-labeled antibody. Thus, conjugation of DTS to monoclonal antibody followed by radiometalation is a simple and efficient method of preparing Tc-99m-labeled monoclonal antibody

  19. Platelet antibodies blood test

    Science.gov (United States)

    This blood test shows if you have antibodies against platelets in your blood. Platelets are a part of the blood ... Chernecky CC, Berger BJ. Platelet antibody - blood. In: Chernecky ... caused by platelet destruction, hypersplenism, or hemodilution. ...

  20. Heavy chain only antibodies

    DEFF Research Database (Denmark)

    Moghimi, Seyed Moein; Rahbarizadeh, Fatemeh; Ahmadvand, Davoud

    2013-01-01

    Unlike conventional antibodies, heavy chain only antibodies derived from camel contain a single variable domain (VHH) and two constant domains (CH2 and CH3). Cloned and isolated VHHs possess unique properties that enable them to excel conventional therapeutic antibodies and their smaller antigen...

  1. Hepatitis A virus antibody

    International Nuclear Information System (INIS)

    Novak, J.; Kselikova, M.; Urbankova, J.

    1980-01-01

    A description is presented of a radioimmunoassay designed to prove the presence of the antibody against the hepatitis A virus (HA Ab, anti-Ha) using an Abbott HAVAB set. This proof as well as the proof of the antibody against the nucleus of the hepatitis B virus is based on competition between a normal antibody against hepatitis A virus and a 125 I-labelled antibody for the binding sites of a specific antigen spread all over the surface of a tiny ball; this is then indirect proof of the antibody under investigation. The method is described of reading the results from the number of impulses per 60 seconds: the higher the titre of the antibody against the hepatitis A virus in the serum examined, the lower the activity of the specimen concerned. The rate is reported of incidence of the antibody against the hepatitis A virus in a total of 68 convalescents after hepatitis A; the antibody was found in 94.1%. The immunoglobulin made from the convalescents' plasma showed the presence of antibodies in dilutions as high as 1:250 000 while the comparable ratio for normal immunoglobulin Norga was only 1:2500. Differences are discussed in the time incidence of the antibodies against the hepatitis A virus, the antibodies against the surface antigen of hepatitis B, and the antibody against the nucleus of the hepatitis V virus. (author)

  2. Anti-insulin antibody test

    Science.gov (United States)

    Insulin antibodies - serum; Insulin Ab test; Insulin resistance - insulin antibodies; Diabetes - insulin antibodies ... Normally, there are no antibodies against insulin in your blood. ... different laboratories. Some labs use different measurements or ...

  3. Monoclonal antibodies and cancer

    International Nuclear Information System (INIS)

    Haisma, H.J.

    1987-01-01

    The usefulness of radiolabeled monoclonal antibodies for imaging and treatment of human (ovarian) cancer was investigated. A review of tumor imaging with monoclonal antibodies is presented. Special attention is given to factors that influence the localization of the antibodies in tumors, isotope choice and methods of radiolabeling of the monoclonal antibodies. Two monoclonal antibodies, OC125 and OV-TL3, with high specificity for human epithelial ovarian cancer are characterized. A simple radio-iodination technique was developed for clinical application of the monoclonal antibodies. The behavior of monoclonal antibodies in human tumor xenograft systems and in man are described. Imaging of tumors is complicated because of high background levels of radioactivity in other sites than the tumor, especially in the bloodpool. A technique was developed to improve imaging of human tumor xenographs in nude mice, using subtraction of a specific and a non-specific antibody, radiolabeled with 111 In, 67 Ga and 131 I. To investigate the capability of the two monoclonal antibodies, to specifically localize in human ovarian carcinomas, distribution studies in mice bearing human ovarian carcinoma xenografts were performed. One of the antibodies, OC125, was used for distribution studies in ovarian cancer patients. OC125 was used because of availability and approval to use this antibody in patients. The same antibody was used to investigate the usefulness of radioimmunoimaging in ovarian cancer patients. The interaction of injected radiolabeled antibody OC125 with circulating antigen and an assay to measure the antibody response in ovarian cancer patients after injection of the antibody is described. 265 refs.; 30 figs.; 19 tabs

  4. PHyTIR - A Prototype Thermal Infrared Radiometer

    Science.gov (United States)

    Jau, Bruno M.; Hook, Simon J.; Johnson, William R.; Foote, Marc C.; Paine, Christopher G.; Pannell, Zack W.; Smythe, Robert F.; Kuan, Gary M.; Jakoboski, Julie K.; Eng, Bjorn T.

    2013-01-01

    This paper describes the PHyTIR (Prototype HyspIRI Thermal Infrared Radiometer) instrument, which is the engineering model for the proposed HyspIRI (Hyperspectral Infrared Imager) earth observing instrument. The HyspIRI mission would be comprised of the HyspIRI TIR (Thermal Infrared Imager), and a VSWIR (Visible Short-Wave Infra-Red Imaging Spectrometer). Both instruments would be used to address key science questions related to the earth's carbon cycle, ecosystems, climate, and solid earth properties. Data gathering of volcanic activities, earthquakes, wildfires, water use and availability, urbanization, and land surface compositions and changes, would aid the predictions and evaluations of such events and the impact they create. Even though the proposed technology for the HyspIRI imager is mature, the PHyTIR prototype is needed to advance the technology levels for several of the instrument's key components, and to reduce risks, in particular to validate 1) the higher sensitivity, spatial resolution, and higher throughput required for this focal plane array, 2) the pointing accuracy, 2) the characteristics of several spectral channels, and 4) the use of ambient temperature optics. The PHyTIR telescope consists of the focal plane assembly that is housed within a cold housing located inside a vacuum enclosure; all mounted to a bulkhead, and an optical train that consists of 3 powered mirrors; extending to both sides of the bulkhead. A yoke connects the telescope to a scan mirror. The rotating mirror enables to scan- a large track on the ground. This structure is supported by kinematic mounts, linking the telescope assembly to a base plate that would also become the spacecraft interface for HyspIRI. The focal plane's cooling units are also mounted to the base plate, as is an overall enclosure that has two viewing ports with large exterior baffles, shielding the focal plane from incoming stray light. PHyTIR's electronics is distributed inside and near the vacuum

  5. Low Average Sidelobe Slot Array Antennas for Radiometer Applications

    Science.gov (United States)

    Rengarajan, Sembiam; Zawardzki, Mark S.; Hodges, Richard E.

    2012-01-01

    In radiometer applications, it is required to design antennas that meet low average sidelobe levels and low average return loss over a specified frequency bandwidth. It is a challenge to meet such specifications over a frequency range when one uses resonant elements such as waveguide feed slots. In addition to their inherent narrow frequency band performance, the problem is exacerbated due to modeling errors and manufacturing tolerances. There was a need to develop a design methodology to solve the problem. An iterative design procedure was developed by starting with an array architecture, lattice spacing, aperture distribution, waveguide dimensions, etc. The array was designed using Elliott s technique with appropriate values of the total slot conductance in each radiating waveguide, and the total resistance in each feed waveguide. Subsequently, the array performance was analyzed by the full wave method of moments solution to the pertinent integral equations. Monte Carlo simulations were also carried out to account for amplitude and phase errors introduced for the aperture distribution due to modeling errors as well as manufacturing tolerances. If the design margins for the average sidelobe level and the average return loss were not adequate, array architecture, lattice spacing, aperture distribution, and waveguide dimensions were varied in subsequent iterations. Once the design margins were found to be adequate, the iteration was stopped and a good design was achieved. A symmetric array architecture was found to meet the design specification with adequate margin. The specifications were near 40 dB for angular regions beyond 30 degrees from broadside. Separable Taylor distribution with nbar=4 and 35 dB sidelobe specification was chosen for each principal plane. A non-separable distribution obtained by the genetic algorithm was found to have similar characteristics. The element spacing was obtained to provide the required beamwidth and close to a null in the E

  6. Improvement of shipborne sky radiometer and its demonstration aboard the Antarctic research vessel Shirase

    Directory of Open Access Journals (Sweden)

    Noriaki Tanaka

    2014-11-01

    Full Text Available The sun-tracking performance of a shipborne sky radiometer was improved to attain accurate aerosol optical thickness (AOT from direct solar measurements on a pitching and rolling vessel. Improvements were made in the accuracy of sun-pointing measurements, field-of-view expansion, sun-tracking speed, and measurement method. Radiometric measurements of direct solar and sky brightness distribution were performed using the shipborne sky radiometer onboard the Antarctic research vessel (R/V Shirase during JARE-51 (2009-2010 and JARE-52 (2010-2011. The temporal variation of signal intensity measured by the radiometer under cloudless conditions was smooth, demonstrating that the radiometer could measure direct sunlight onboard the R/V. AOT at 500 nm ranged from 0.01 to 0.34, and values over Southeast Asia and over the western Pacific Ocean in spring were higher than those over other regions. The Angstrom exponent ranged from -0.06 to 2.00, and values over Southeast Asia and off the coast near Sydney were the highest. The improved shipborne sky radiometer will contribute to a good understanding of the nature of aerosols over the ocean.

  7. [VGKC-complex antibodies].

    Science.gov (United States)

    Watanabe, Osamu

    2013-04-01

    Various antibodies are associated with voltage-gated potassium channels (VGKCs). Representative antibodies to VGKCs were first identified by radioimmunoassays using radioisotope-labeled alpha-dendrotoxin-VGKCs solubilized from rabbit brain. These antibodies were detected only in a proportion of patients with acquired neuromyotonia (Isaacs' syndrome). VGKC antibodies were also detected in patients with Morvan's syndrome and in those with a form of autoimmune limbic encephalitis. Recent studies indicated that the "VGKC" antibodies are mainly directed toward associated proteins (for example LGI-1 and CASPR-2) that complex with the VGKCs themselves. The "VGKC" antibodies are now commonly known as VGKC-complex antibodies. In general, LGI-1 antibodies are most commonly detected in patients with limbic encephalitis with syndrome of inappropriate secretion of antidiuretic hormone. CASPR-2 antibodies are present in the majority of patients with Morvan's syndrome. These patients develop combinations of CNS symptoms, autonomic dysfunction, and peripheral nerve hyperexcitability. Furthermore, VGKC-complex antibodies are tightly associated with chronic idiopathic pain. Hyperexcitability of nociceptive pathways has also been implicated. These antibodies may be detected in sera of some patients with neurodegenerative diseases (for example, amyotrophic lateral sclerosis and Creutzfeldt-Jakob disease).

  8. Specificity and polyreactivity of the antibody response during natural HIV-1 infection

    OpenAIRE

    Wang, Xin

    2006-01-01

    The specificity and polyreactivity of the antibody response in natural HIV-1 infection were studied. First, to investigate the overall antibody response, overlapping linear peptides were used to screen sera taken from HIV-1-infected individuals. The polyclonal antibody response was relatively stable during long-term infection, compared with acute infection, and mostly directed against immunodominant regions. Low level, transient antibody responses were detected against membrane proximal exter...

  9. Radiolabeled antibody imaging

    International Nuclear Information System (INIS)

    Wahl, R.L.

    1987-01-01

    Radiolabeled antibodies, in particular monoclonal antibodies, offer the potential for the specific nuclear imaging of malignant and benign diseases in man. If this imaging potential is realized, they may also have a large role in cancer treatment. This paper reviews: (1) what monoclonal antibodies are and how they differ from polyclonal antibodies, (2) how they are produced and radiolabeled, (3) the results of preclinical and clinical trials in cancer imaging, including the utility of SPECT and antibody fragments, (4) the role of antibodies in the diagnosis of benign diseases, (5) alternate routes of antibody delivery, (6) the role of these agents in therapy, and (7) whether this technology ''revolutionizes'' the practice of nuclear radiology, or has a more limited complementary role in the imaging department

  10. Stable Boundary Layer Issues

    OpenAIRE

    Steeneveld, G.J.

    2012-01-01

    Understanding and prediction of the stable atmospheric boundary layer is a challenging task. Many physical processes are relevant in the stable boundary layer, i.e. turbulence, radiation, land surface coupling, orographic turbulent and gravity wave drag, and land surface heterogeneity. The development of robust stable boundary layer parameterizations for use in NWP and climate models is hampered by the multiplicity of processes and their unknown interactions. As a result, these models suffer ...

  11. New improved algorithm for sky calibration of L-band radiometers JLBARA and ELBARA II

    KAUST Repository

    Dimitrov, Marin; Kostov, K. G.; Jonard, Franç ois; Jadoon, Khan; Schwank, Mike; Weihermü ller, Lutz; Hermes, Normen; Vanderborght, Jan P.; Vereecken, Harry

    2012-01-01

    We propose a new algorithm for sky calibration of the L-band radiometers JLBARA and ELBARA II, introducing the effective transmissivities of the instruments. The suggested approach was tested using experimental data obtained at the Selhausen test site, Germany. It was shown that for JLBARA the effective transmissivities depend strongly on the air temperature and decrease with increasing air temperature, while for ELBARA II such strong dependence was not observed. It was also shown that the effective transmissivities account for the antenna and feed cable loss effects, and for the variations of the radiometer gain due to air temperature changes. The new calibration algorithm reduces significantly the bias of brightness temperature estimates for both radiometers, especially for JLBARA. © 2012 IEEE.

  12. New improved algorithm for sky calibration of L-band radiometers JLBARA and ELBARA II

    KAUST Repository

    Dimitrov, Marin

    2012-03-01

    We propose a new algorithm for sky calibration of the L-band radiometers JLBARA and ELBARA II, introducing the effective transmissivities of the instruments. The suggested approach was tested using experimental data obtained at the Selhausen test site, Germany. It was shown that for JLBARA the effective transmissivities depend strongly on the air temperature and decrease with increasing air temperature, while for ELBARA II such strong dependence was not observed. It was also shown that the effective transmissivities account for the antenna and feed cable loss effects, and for the variations of the radiometer gain due to air temperature changes. The new calibration algorithm reduces significantly the bias of brightness temperature estimates for both radiometers, especially for JLBARA. © 2012 IEEE.

  13. Stable isotopes labelled compounds

    International Nuclear Information System (INIS)

    1982-09-01

    The catalogue on stable isotopes labelled compounds offers deuterium, nitrogen-15, and multiply labelled compounds. It includes: (1) conditions of sale and delivery, (2) the application of stable isotopes, (3) technical information, (4) product specifications, and (5) the complete delivery programme

  14. Evolutionary Stable Strategy

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 21; Issue 9. Evolutionary Stable Strategy: Application of Nash Equilibrium in Biology. General Article Volume 21 Issue 9 September 2016 pp 803- ... Keywords. Evolutionary game theory, evolutionary stable state, conflict, cooperation, biological games.

  15. Stable Boundary Layer Issues

    NARCIS (Netherlands)

    Steeneveld, G.J.

    2012-01-01

    Understanding and prediction of the stable atmospheric boundary layer is a challenging task. Many physical processes are relevant in the stable boundary layer, i.e. turbulence, radiation, land surface coupling, orographic turbulent and gravity wave drag, and land surface heterogeneity. The

  16. Antibody recognition of Z-DNA

    International Nuclear Information System (INIS)

    Lafer, E.M.; Moeller, A.; Valle, R.P.C.; Nordheim, V.A.; Rich, A.; Stollar, B.D.; Massachusetts Inst. of Tech., Cambridge)

    1983-01-01

    To measure serological reactions under physiological ionic strength, we prepared a brominated (Bl) poly(dG-dC).poly(dG-dC), which forms a stable Z helix in solutions of low salt concentration. Mice and rabbits were immunized with this polymer complexed with the basic protein methylated bovine serum albumin (MBSA), and it was discovered that the Z-DNA helix is a strong immunogen. Various antibody populations were purified from the rabbit serum by quantitative immunoprecipitation. Spleen cells from the mice were used for the preparation of hybridoma cell lines secreting monoclonal antibodies. Anti-Z-DNA antibodies were also raised by immunizing animals with poly(dG-dm 5 C).poly(dG-dm 5 C) under conditions where it was reported to be in the left-handed Z conformation as well as unmodified poly(dG-dC).poly(dG-dC) that was in the right-handed B conformation: both were complexed with MBSA. Z-DNA reactive antibodies were found in both murine and human SLE. A Z-DNA-specific as well as a dDNA and Z-DNA cross-reactive antibody population were distinguished by affinity chromatography of the SLE sera. The specificities of the various anti-Z-DNA antibody populations were measured by direct-binding and competitive radioimmunoassays, using synthetic polymers of defined structure under various ionic strengths. These studies allow us to map the possible antigenic sites for these antibodies, which serve as a model for DNA-protein recognition. The findings also established the usefulness of the antibodies as biochemical probes for Z-DNA. 29 references, 6 figures, 1 table

  17. Description and Performance of an L-Band Radiometer with Digital Beamforming

    Directory of Open Access Journals (Sweden)

    Juan F. Marchan-Hernandez

    2010-12-01

    Full Text Available This paper presents the description and performance tests of an L-band microwave radiometer with Digital Beamforming (DBF, developed for the Passive Advanced Unit (PAU for ocean monitoring project. PAU is an instrument that combines, in a single receiver and without time multiplexing, a microwave radiometer at L-band (PAU-RAD and a GPS-reflectometer (PAU-GNSS-R. This paper focuses on the PAU‑RAD beamformer’s first results, analyzing the hardware and software required for the developed prototype. Finally, it discusses the first results measured in the Universitat Politècnica de Catalunya (UPC anechoic chamber.

  18. Development of an improved Newtonian total radiometer, its evaluation and calibration

    International Nuclear Information System (INIS)

    Castrejon G, R.; Morales, A.

    1998-01-01

    Measuring of radiant energy by optical non intrusive means is an important topic of research in many areas of science and technology. Precise evaluation of thermal energy emitted by hot bodies leads to a better understanding of the energy interchange phenomena between the body and its surroundings. To this end, a wide spectrum optical radiometer was developed. In this article we describe the construction and evaluation of this instrument and the physical principles involved in its design and operation. Among other advantages, the linear response of the instrument allows easily a precise calibration. Additionally, we give a procedure to obtain a known source of radiation that was used to calibrate the radiometer. (Author)

  19. The Along Track Scanning Radiometer (ATSR) - Orbital performance and future developments

    Science.gov (United States)

    Sandford, M. C. W.; Edwards, T.; Mutlow, C. T.; Delderfield, J.; Llewellyn-Jones, D. T.

    1992-08-01

    The Along-Track Scanning Radiometer (ATSR), a new kind of infrared radiometer which is intended to make sea surface temperature measurements with an absolute accuracy of +/- 0.5 K averaged over cells of 0.5 deg in latitude, is discussed. The ATSR employs four detectors centered at 12, 11, 3.7, and 1.6 microns. The noise performance thermal performance, and Stirling cycle cooler performance of the ATSR on ERS-1 are examined along with 3.7 micron channel results. The calibration, structure, and data handling of the ATSRs planned for ERS-2 and for the POEM mission are examined.

  20. Upgraded ECE radiometer on the Tore Supra Tokamak

    International Nuclear Information System (INIS)

    Segui, J.L.; Molina, D.; Goniche, M.; Maget, P.; Udintsev, V.S.; Kraemer-Flecken, A.

    2004-01-01

    An upgraded 32-channel heterodyne radiometer, 1 GHz spaced, is used on the Tore-Supra tokamak to measure the electron cyclotron emission (ECE) in the frequency range 78-110 GHz for the ordinary mode (O1) and 94-126.5 GHz for the extraordinary mode (X2). From now radial resolution is essentially limited by ECE relativistic effects related to electron temperature and density, not by the channels frequency spacing. For example, this leads to precise electron temperature mapping during magneto hydrodynamic activities (MHD). In the equatorial plane, we use a dual polarisation Gaussian optics lens antenna. It has low spreading and a perpendicular line-of-sight that gives ECE measurements very low refraction and Doppler effects. Assuming that the plasma is a black body and there is no overlap between ECE harmonics, one can deduce the electron temperature profile by using the first harmonic ordinary mode (O1) or the second harmonic extraordinary mode (X2). The principle radio frequency emitter (RF) has its frequencies down shifted into intermediary frequencies (IF) that span from 2 to 18 GHz in the single side band mode (SSB). It is amplified by low noise IF amplifiers before forming channels. A separate O/X mode RF front-end allows the use of an IF electronic mode selector. This gives the potentiality of simultaneous O/X mode measurements in the 94-110 GHz. RF and IF filters reject the gyrotron frequency (118 GHz) in order to perform electron temperature measurements during electron cyclotron resonance heated plasmas. A precise absolute spectral calibration is performed outside the tokamak vacuum vessel by using a 600 deg C black body hot source, a double coherent digital signal averaging (trigger, turn and clock) on the waveform generated by a mechanical chopper, and a simulated tokamak window. The use of differential electronics and strong electromagnetic shielding improves also the calibration precision. The fast and slow data acquisition systems are free of aliasing

  1. Upgraded ECE radiometer on the Tore Supra Tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Segui, J.L.; Molina, D.; Goniche, M.; Maget, P.; Udintsev, V.S. [Association Euratom-CEA, Centre d' Etudes de Cadarache, 13 - Saint-Paul-lez-Durance (France). Dept. de Recherches sur la Fusion Controlee; Antar, G.Y. [Center for Energy Research, UCSD, La Jolla CA (United States); Kraemer-Flecken, A. [Forschungszentrum Juelich GmbH (Germany). Inst. fuer Plasmaphysik

    2004-07-01

    An upgraded 32-channel heterodyne radiometer, 1 GHz spaced, is used on the Tore-Supra tokamak to measure the electron cyclotron emission (ECE) in the frequency range 78-110 GHz for the ordinary mode (O1) and 94-126.5 GHz for the extraordinary mode (X2). From now radial resolution is essentially limited by ECE relativistic effects related to electron temperature and density, not by the channels frequency spacing. For example, this leads to precise electron temperature mapping during magneto hydrodynamic activities (MHD). In the equatorial plane, we use a dual polarisation Gaussian optics lens antenna. It has low spreading and a perpendicular line-of-sight that gives ECE measurements very low refraction and Doppler effects. Assuming that the plasma is a black body and there is no overlap between ECE harmonics, one can deduce the electron temperature profile by using the first harmonic ordinary mode (O1) or the second harmonic extraordinary mode (X2). The principle radio frequency emitter (RF) has its frequencies down shifted into intermediary frequencies (IF) that span from 2 to 18 GHz in the single side band mode (SSB). It is amplified by low noise IF amplifiers before forming channels. A separate O/X mode RF front-end allows the use of an IF electronic mode selector. This gives the potentiality of simultaneous O/X mode measurements in the 94-110 GHz. RF and IF filters reject the gyrotron frequency (118 GHz) in order to perform electron temperature measurements during electron cyclotron resonance heated plasmas. A precise absolute spectral calibration is performed outside the tokamak vacuum vessel by using a 600 deg C black body hot source, a double coherent digital signal averaging (trigger, turn and clock) on the waveform generated by a mechanical chopper, and a simulated tokamak window. The use of differential electronics and strong electromagnetic shielding improves also the calibration precision. The fast and slow data acquisition systems are free of aliasing

  2. Normal modified stable processes

    DEFF Research Database (Denmark)

    Barndorff-Nielsen, Ole Eiler; Shephard, N.

    2002-01-01

    Gaussian (NGIG) laws. The wider framework thus established provides, in particular, for added flexibility in the modelling of the dynamics of financial time series, of importance especially as regards OU based stochastic volatility models for equities. In the special case of the tempered stable OU process......This paper discusses two classes of distributions, and stochastic processes derived from them: modified stable (MS) laws and normal modified stable (NMS) laws. This extends corresponding results for the generalised inverse Gaussian (GIG) and generalised hyperbolic (GH) or normal generalised inverse...

  3. Applications of stable isotopes

    International Nuclear Information System (INIS)

    Letolle, R.; Mariotti, A.; Bariac, T.

    1991-06-01

    This report reviews the historical background and the properties of stable isotopes, the methods used for their measurement (mass spectrometry and others), the present technics for isotope enrichment and separation, and at last the various present and foreseeable application (in nuclear energy, physical and chemical research, materials industry and research; tracing in industrial, medical and agronomical tests; the use of natural isotope variations for environmental studies, agronomy, natural resources appraising: water, minerals, energy). Some new possibilities in the use of stable isotope are offered. A last chapter gives the present state and forecast development of stable isotope uses in France and Europe

  4. Development of a High-Stability Microstrip-based L-band Radiometer for Ocean Salinity Measurements

    Science.gov (United States)

    Pellerano, Fernando A.; Horgan, Kevin A.; Wilson, William J.; Tanner, Alan B.

    2004-01-01

    The development of a microstrip-based L-band Dicke radiometer with the long-term stability required for future ocean salinity measurements to an accuracy of 0.1 psu is presented. This measurement requires the L-band radiometers to have calibration stabilities of less than or equal to 0.05 K over 2 days. This research has focused on determining the optimum radiometer requirements and configuration to achieve this objective. System configuration and component performance have been evaluated with radiometer test beds at both JPL and GSFC. The GSFC testbed uses a cryogenic chamber that allows long-term characterization at radiometric temperatures in the range of 70 - 120 K. The research has addressed several areas including component characterization as a function of temperature and DC bias, system linearity, optimum noise diode injection calibration, and precision temperature control of components. A breadboard radiometer, utilizing microstrip-based technologies, has been built to demonstrate this long-term stability.

  5. Deriving a sea surface temperature record suitable for climate change research from the along-track scanning radiometers

    Science.gov (United States)

    Merchant, C. J.; Llewellyn-Jones, D.; Saunders, R. W.; Rayner, N. A.; Kent, E. C.; Old, C. P.; Berry, D.; Birks, A. R.; Blackmore, T.; Corlett, G. K.; Embury, O.; Jay, V. L.; Kennedy, J.; Mutlow, C. T.; Nightingale, T. J.; O'Carroll, A. G.; Pritchard, M. J.; Remedios, J. J.; Tett, S.

    We describe the approach to be adopted for a major new initiative to derive a homogeneous record of sea surface temperature for 1991 2007 from the observations of the series of three along-track scanning radiometers (ATSRs). This initiative is called (A)RC: (Advanced) ATSR Re-analysis for Climate. The main objectives are to reduce regional biases in retrieved sea surface temperature (SST) to less than 0.1 K for all global oceans, while creating a very homogenous record that is stable in time to within 0.05 K decade-1, with maximum independence of the record from existing analyses of SST used in climate change research. If these stringent targets are achieved, this record will enable significantly improved estimates of surface temperature trends and variability of sufficient quality to advance questions of climate change attribution, climate sensitivity and historical reconstruction of surface temperature changes. The approach includes development of new, consistent estimators for SST for each of the ATSRs, and detailed analysis of overlap periods. Novel aspects of the approach include generation of multiple versions of the record using alternative channel sets and cloud detection techniques, to assess for the first time the effect of such choices. There will be extensive effort in quality control, validation and analysis of the impact on climate SST data sets. Evidence for the plausibility of the 0.1 K target for systematic error is reviewed, as is the need for alternative cloud screening methods in this context.

  6. Analysing Stable Time Series

    National Research Council Canada - National Science Library

    Adler, Robert

    1997-01-01

    We describe how to take a stable, ARMA, time series through the various stages of model identification, parameter estimation, and diagnostic checking, and accompany the discussion with a goodly number...

  7. The state-of-play and future of antibody therapeutics.

    Science.gov (United States)

    Elgundi, Zehra; Reslan, Mouhamad; Cruz, Esteban; Sifniotis, Vicki; Kayser, Veysel

    2017-12-01

    It has been over four decades since the development of monoclonal antibodies (mAbs) using a hybridoma cell line was first reported. Since then more than thirty therapeutic antibodies have been marketed, mostly as oncology, autoimmune and inflammatory therapeutics. While antibodies are very efficient, their cost-effectiveness has always been discussed owing to their high costs, accumulating to more than one billion dollars from preclinical development through to market approval. Because of this, therapeutic antibodies are inaccessible to some patients in both developed and developing countries. The growing interest in biosimilar antibodies as affordable versions of therapeutic antibodies may provide alternative treatment options as well potentially decreasing costs. As certain markets begin to capitalize on this opportunity, regulatory authorities continue to refine the requirements for demonstrating quality, efficacy and safety of biosimilar compared to originator products. In addition to biosimilars, innovations in antibody engineering are providing the opportunity to design biobetter antibodies with improved properties to maximize efficacy. Enhancing effector function, antibody drug conjugates (ADC) or targeting multiple disease pathways via multi-specific antibodies are being explored. The manufacturing process of antibodies is also moving forward with advancements relating to host cell production and purification processes. Studies into the physical and chemical degradation pathways of antibodies are contributing to the design of more stable proteins guided by computational tools. Moreover, the delivery and pharmacokinetics of antibody-based therapeutics are improving as optimized formulations are pursued through the implementation of recent innovations in the field. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. On-board digital RFI and polarimetry processor for future spaceborne radiometer systems

    DEFF Research Database (Denmark)

    Skou, Niels; Kristensen, Steen Savstrup; Ruokokoski, T.

    2012-01-01

    Man-made Radio Frequency Interference (RFI) is an increasingly threatening problem for passive microwave radiometry from space. The problem is presently very evident in L-band data from SMOS, but it is realized that it is already now a problem at other traditional radiometer bands at C, X, and Ku...

  9. Inspection of feasible calibration conditions for UV radiometer detectors with the KI/KIO3 actinometer.

    Science.gov (United States)

    Qiang, Zhimin; Li, Wentao; Li, Mengkai; Bolton, James R; Qu, Jiuhui

    2015-01-01

    UV radiometers are widely employed for irradiance measurements, but their periodical calibrations not only induce an extra cost but also are time-consuming. In this study, the KI/KIO3 actinometer was applied to calibrate UV radiometer detectors at 254 nm with a quasi-collimated beam apparatus equipped with a low-pressure UV lamp, and feasible calibration conditions were identified. Results indicate that a washer constraining the UV light was indispensable, while the size (10 or 50 mL) of a beaker containing the actinometer solution had little influence when a proper washer was used. The absorption or reflection of UV light by the internal beaker wall led to an underestimation or overestimation of the irradiance determined by the KI/KIO3 actinometer, respectively. The proper range of the washer internal diameter could be obtained via mathematical analysis. A radiometer with a longer service time showed a greater calibration factor. To minimize the interference from the inner wall reflection of the collimating tube, calibrations should be conducted at positions far enough away from the tube bottom. This study demonstrates that after the feasible calibration conditions are identified, the KI/KIO3 actinometer can be applied readily to calibrate UV radiometer detectors at 254 nm. © 2014 The American Society of Photobiology.

  10. Polarimetric Signatures from a Crop Covered Land Surface Measured by an L-band Polarimetric Radiometer

    DEFF Research Database (Denmark)

    Søbjærg, Sten Schmidl; Skou, Niels

    2003-01-01

    This paper describes preliminary results from field measurements of polarimetric azimuth signatures with the EMIRAD L-band polarimetric radiometer, performed over a land test site at the Institut National de la Recherche Agronomique in Avignon, France. Scans of 180 degrees in azimuth were carried...

  11. Design of a rocket-borne radiometer for stratospheric ozone measurements

    International Nuclear Information System (INIS)

    Barnes, R.A.; Simeth, P.G.

    1989-01-01

    A four-filter ultraviolet radiometer for measuring stratospheric ozone is described. The payload is launched aboard a Super-Loki rocket to an apogee of 70 km. The instrument measures the solar ultraviolet irradiance over its filter wavelengths as it descends on a parachute. The amount of ozone in the path between the radiometer and the sun is calculated from the attenuation of solar flux using the Beer-Lambert law. Radar at the launch site measures the height of the instrument throughout its flight. The fundamental ozone value measured by the ROCOZ-A radiometer is the vertical ozone overburden as a function of geometric altitude. Ozone measurements are obtained for altitudes from 55 to 20 km, extending well above the altitude range of balloon-borne ozone-measuring instruments. The optics and electronics in the radiometer have been designed within relatively severe size and weight limitations imposed by the launch vehicle. The electronics in the improved rocket ozonesonde (ROCOZ-A) provide essentially drift-free outputs throughout 40-min ozone soundings at stratospheric temperatures. The modest cost of the payload precludes recovery and makes the instrument a versatile tool compared to larger ozonesondes

  12. L-Band Radiometers Measuring Salinity From Space: Atmospheric Propagation Effects

    DEFF Research Database (Denmark)

    Skou, Niels; Hofman-Bang, Dorthe

    2005-01-01

    Microwave radiometers can measure sea surface salinity from space using L-band frequencies around 1.4 GHz. However, requirements to the accuracy of the measurements, in order to be satisfactory for the user, are so stringent that the influence of the intervening atmosphere cannot be neglected...

  13. Insolation measurements with a portable CuS-CdS radiometer

    Science.gov (United States)

    Windawi, H. M.

    1976-01-01

    Solar radiation measurements were carried out with a portable Cu2S-Cds radiometer. The measurements were found to be accurate to better than 5% (better than 3% when sophisticated metering is employed). Calibration to an Eppley precision pyranometer is discussed.

  14. Hybrid PSO-ASVR-based method for data fitting in the calibration of infrared radiometer

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Sen; Li, Chengwei, E-mail: heikuanghit@163.com [School of Electrical Engineering and Automation, Harbin Institute of Technology, Harbin 150001 (China)

    2016-06-15

    The present paper describes a hybrid particle swarm optimization-adaptive support vector regression (PSO-ASVR)-based method for data fitting in the calibration of infrared radiometer. The proposed hybrid PSO-ASVR-based method is based on PSO in combination with Adaptive Processing and Support Vector Regression (SVR). The optimization technique involves setting parameters in the ASVR fitting procedure, which significantly improves the fitting accuracy. However, its use in the calibration of infrared radiometer has not yet been widely explored. Bearing this in mind, the PSO-ASVR-based method, which is based on the statistical learning theory, is successfully used here to get the relationship between the radiation of a standard source and the response of an infrared radiometer. Main advantages of this method are the flexible adjustment mechanism in data processing and the optimization mechanism in a kernel parameter setting of SVR. Numerical examples and applications to the calibration of infrared radiometer are performed to verify the performance of PSO-ASVR-based method compared to conventional data fitting methods.

  15. An RFI Detection Algorithm for Microwave Radiometers Using Sparse Component Analysis

    Science.gov (United States)

    Mohammed-Tano, Priscilla N.; Korde-Patel, Asmita; Gholian, Armen; Piepmeier, Jeffrey R.; Schoenwald, Adam; Bradley, Damon

    2017-01-01

    Radio Frequency Interference (RFI) is a threat to passive microwave measurements and if undetected, can corrupt science retrievals. The sparse component analysis (SCA) for blind source separation has been investigated to detect RFI in microwave radiometer data. Various techniques using SCA have been simulated to determine detection performance with continuous wave (CW) RFI.

  16. Mapping of the DOME-C area in Antarctica by an airborne L-band radiometer

    DEFF Research Database (Denmark)

    Skou, Niels; Kristensen, Steen Savstrup; Søbjærg, Sten Schmidl

    2014-01-01

    A 350 × 350 km area near the Concordia station on the high plateau of Dome C in Antarctica has been mapped by an airborne L-band radiometer system. The area was expected to display a rather uniform brightness temperature close to the yearly mean temperature — well suited for calibration checks...

  17. Airborne L-band radiometer mapping of the dome-C area in Antarctica

    DEFF Research Database (Denmark)

    Skou, Niels; Kristensen, Steen Savstrup; Søbjærg, Sten Schmidl

    2015-01-01

    A 350 km × 350 km area near the Concordia station on the high plateau of Dome-C in Antarctica has been mapped by an airborne L-band radiometer system. The area was expected to display a rather uniform brightness temperature (TB) close to the yearly mean temperature-well suited for calibration...

  18. InP HEMT Integrated Circuits for Submillimeter Wave Radiometers in Earth Remote Sensing

    Science.gov (United States)

    Deal, William R.; Chattopadhyay, Goutam

    2012-01-01

    The operating frequency of InP integrated circuits has pushed well into the Submillimeter Wave frequency band, with amplification reported as high as 670 GHz. This paper provides an overview of current performance and potential application of InP HEMT to Submillimeter Wave radiometers for earth remote sensing.

  19. Validation of multi-channel scanning microwave radiometer on-board Oceansat-1

    Digital Repository Service at National Institute of Oceanography (India)

    Muraleedharan, P.M.; Pankajakshan, T.; Harikrishnan, M.

    Sea surface temperature (SST), sea surface wind speed (WS) and columnar water vapour (WV) derived from Multi-frequency Scanning Microwave Radiometer (MSMR) sensor on-board IRS-P4 (Oceansat-1) were validated against the in situ measurements from ship...

  20. Thermal, Thermophysical, and Compositional Properties of the Moon Revealed by the Diviner Lunar Radiometer

    Science.gov (United States)

    Greenhagen, B. T.; Paige, D. A.

    2012-01-01

    The Diviner Lunar Radiometer is the first multispectral thermal instrument to globally map the surface of the Moon. After over three years in operation, this unprecedented dataset has revealed the extreme nature of the Moon's thermal environment, thermophysical properties, and surface composition.

  1. Ultra-portable field transfer radiometer for vicarious calibration of earth imaging sensors

    Science.gov (United States)

    Thome, Kurtis; Wenny, Brian; Anderson, Nikolaus; McCorkel, Joel; Czapla-Myers, Jeffrey; Biggar, Stuart

    2018-06-01

    A small portable transfer radiometer has been developed as part of an effort to ensure the quality of upwelling radiance from test sites used for vicarious calibration in the solar reflective. The test sites are used to predict top-of-atmosphere reflectance relying on ground-based measurements of the atmosphere and surface. The portable transfer radiometer is designed for one-person operation for on-site field calibration of instrumentation used to determine ground-leaving radiance. The current work describes the detector- and source-based radiometric calibration of the transfer radiometer highlighting the expected accuracy and SI-traceability. The results indicate differences between the detector-based and source-based results greater than the combined uncertainties of the approaches. Results from recent field deployments of the transfer radiometer using a solar radiation based calibration agree with the source-based laboratory calibration within the combined uncertainties of the methods. The detector-based results show a significant difference to the solar-based calibration. The source-based calibration is used as the basis for a radiance-based calibration of the Landsat-8 Operational Land Imager that agrees with the OLI calibration to within the uncertainties of the methods.

  2. Soil Moisture Active/Passive (SMAP) Radiometer Subband Calibration and Calibration Drift

    Science.gov (United States)

    Peng, Jinzheng; Piepmeier, Jeffrey R.; De Amici, Giovanni; Mohammed, Priscilla

    2016-01-01

    The SMAP is one of four first-tier missions recommended by the US National Research Council's Committee on Earth Science and Applications from Space (Earth Science and Applications from Space: National Imperatives for the Next Decade and Beyond, Space Studies Board, National Academies Press, 2007)]. The observatory was launched on Jan 31, 2015. The goal of the SMAP is to measure the global soil moisture and freeze/thaw from space. The L-band radiometer is the passive portion of the spaceborne instrument. It measures all four Stokes antenna temperatures and outputs counts. The Level 1B Brightness Temperature (L1B_TB) science algorithm converts radiometer counts to the Earths surface brightness temperature. The results are reported in the radiometer level 1B data product together with the calibrated antenna temperature (TA) and all of the corrections to the unwanted sources contribution. The calibrated L1B data product are required to satisfy the overall radiometer error budget of 1.3 K needed to meet the soil moisture requirement of 0.04 volumetric fraction uncertainty and the calibration drift requirement of no larger than 0.4 K per month.

  3. Soil Moisture Active Passive (SMAP) Radiometer Subband Calibration and Calibration Drift

    Science.gov (United States)

    Peng, Jinzheng; Piepmeier, Jeffrey R.; De Amici, Giovanni; Mohammed, Priscilla N.

    2016-01-01

    The SMAP is one of four first-tier missions recommended by the US National Research Council's Committee on Earth Science and Applications from Space (Earth Science and Applications from Space: National Imperatives for the Next Decade and Beyond, Space Studies Board, National Academies Press, 2007). The observatory was launched on Jan 31, 2015. The goal of the SMAP is to measure the global soil moisture and freeze/thaw from space. The L-band radiometer is the passive portion of the spaceborne instrument. It measures all four Stokes antenna temperatures and outputs counts. The Level 1B Brightness Temperature (L1B_TB) science algorithm converts radiometer counts to the Earths surface brightness temperature. The results are reported in the radiometer level 1B data product together with the calibrated antenna temperature (TA) and all of the corrections to the unwanted sources contribution. The calibrated L1B data product are required to satisfy the overall radiometer error budget of 1.3 K needed to meet the soil moisture requirement of 0.04 volumetric fraction uncertainty and the calibration drift requirement of no larger than 0.4 K per month.

  4. A brief comparison of radiometers at NSIDC and their potential to generate long ESDRs

    Science.gov (United States)

    Moth, P.; Johnston, T.; Haran, T. M.; Fowler, D. K.

    2017-12-01

    Radiometers have played a big part in Earth observing science. In this poster we compare three such instruments: the Advanced Very-High-resolution Radiometer (AVHRR), the Moderate Resolution Imaging Spectroradiometer (MODIS), and the Visible Infrared Imaging Radiometer Suite (VIIRS). The NASA National Snow and Ice Distributed Active Archive Center (NSIDC DAAC) has archived cryospheric data from all three of these instruments. AVHRR was a 4-channel radiometer that was first launched in 1978 aboard the TIROS-N satellite. Subsequent missions launched improved versions of AVHRR with five and six channels, observing Earth in frequencies ranging from 0.58 μm to 12.5 μm with a resolution at nadir of 1.09 km. MODIS instruments fly onboard NASA's Earth Observing System (EOS) Terra and Aqua satellites. Launched in 1999 and 2002, respectively, they still produce much sought after data observed in 36 spectral bands ranging from 0.4 μm to 14.4 μm. Two bands image Earth at a nominal resolution of 250 m at nadir, five at 500 m, and the remaining 29 bands at 1 km. A ±55-degree scanning pattern at the sun-synchronous orbit of 705 km achieves a 2,330 km swath and provides global coverage every one to two days VIIRS, NOAA's latest radiometer, was launched aboard the Suomi National Polar-orbiting Partnership satellite on October 28, 2011. Working collaboratively, NASA and NOAA are producing data that is archived and distributed via NASA DAACs. The VIIRS radiometer comprises 22 bands; five for high-resolution imagery, 16 at moderate resolution, and one panchromatic day/night band. VIIRS is a whiskbroom scanning radiometer that covers the spectrum between 0.412 μm and 12.01 μm and acquires spatial resolutions at nadir of 750 m, 375 m, and 750 m, respectively. Although these instruments are configured with different spectral bands, each was designed with an eye to the future. MODIS can be thought of as a successor to the AVHRR mission, adding capabilities that yielded better data

  5. Therapeutic Recombinant Monoclonal Antibodies

    Science.gov (United States)

    Bakhtiar, Ray

    2012-01-01

    During the last two decades, the rapid growth of biotechnology-derived techniques has led to a myriad of therapeutic recombinant monoclonal antibodies with significant clinical benefits. Recombinant monoclonal antibodies can be obtained from a number of natural sources such as animal cell cultures using recombinant DNA engineering. In contrast to…

  6. Expression of recombinant Antibodies

    Directory of Open Access Journals (Sweden)

    André eFrenzel

    2013-07-01

    Full Text Available Recombinant antibodies are highly specific detection probes in research, diagnostics and have emerged over the last two decades as the fastest growing class of therapeutic proteins. Antibody generation has been dramatically accelerated by in vitro selection systems, particularly phage display. An increasing variety of recombinant production systems have been developed, ranging from Gram-negative and positive bacteria, yeasts and filamentous fungi, insect cell lines, mammalian cells to transgenic plants and animals. Currently, almost all therapeutic antibodies are still produced in mammalian cell lines in order to reduce the risk of immunogenicity due to altered, non-human glycosylation patterns. However, recent developments of glycosylation-engineered yeast, insect cell lines and transgenic plants are promising to obtain antibodies with human-like post-translational modifications. Furthermore, smaller antibody fragments including bispecific antibodies without any glycosylation are successfully produced in bacteria and have advanced to clinical testing. The first therapeutic antibody products from a non-mammalian source can be expected in coming next years. In this review, we focus on current antibody production systems including their usability for different applications.

  7. Stability of rhenium-188 labeled antibody

    International Nuclear Information System (INIS)

    Lim, B. K.; Jung, J. M.; Jung, J. K.; Lee, D. S.; Lee, M. C.

    1999-01-01

    For clinical application of beta-emitter labeled antibody, high specific activity is important. Carrier-free Re-188 from W-188/Re-188 generator is an ideal radionuclide for this purpose. However, low stability of Re-188 labeled antibody, especially in high specific activity, due to radiolytic decomposition by high energy (2.1 MeV) beta ray was problem. We studied the stability of Re-188 labeled antibody, and stabilizing effect of several nontoxic radical-quenching agents. Pre-reduced monoclonal antibody (CEA79.4) was labeled with Re-188 by incubating with generator-eluted Re-188-perrhenate in the presence of stannous tartrate for 2 hr at room temperature. Radiochemical purity of each preparation was determined by chromatography (ITLC-SG/acetone, ITLC-SG/Umezawa, Whatman No.1/saline). Human serum albumin was added to the labeled antibodies(2%). Stability of Re-188-CEA79.4 was investigated in the presence of vitamin C, ethanol, or Tween 80 as radical-quenching agents. Specific activities of 4.29∼5.11 MBq/μg were obtained. Labeling efficiencies were 88±4%(n=12). Very low stability after removal of stannous tartrate from the preparation was observed. If stored after purging with N 2 , all the preparations were stable for 10 hr. However, if contacted with air, stability decreased. Perrhenate and Re-188-tartrate was major impurity in declined preparation (12∼47 and 9∼38% each, after 10 hr). Colloid-formation was not a significant problem in all cases. Addition of vitamin C stabilized the labeled antibodies either under N 2 or under air by reducing the formation of perrhenate. High specific activity Re-188 labeled antibody is unstable, especially, in the presence of oxygen. Addition of vitamin C increased the stability

  8. Improved characterization of scenes with a combination of MMW radar and radiometer information

    Science.gov (United States)

    Dill, Stephan; Peichl, Markus; Schreiber, Eric; Anglberger, Harald

    2017-05-01

    For security related applications MMW radar and radiometer systems in remote sensing or stand-off configurations are well established techniques. The range of development stages extends from experimental to commercial systems on the civil and military market. Typical examples are systems for personnel screening at airports for concealed object detection under clothing, enhanced vision or landing aid for helicopter and vehicle based systems for suspicious object or IED detection along roads. Due to the physical principle of active (radar) and passive (radiometer) MMW measurement techniques the appearance of single objects and thus the complete scenario is rather different for radar and radiometer images. A reasonable combination of both measurement techniques could lead to enhanced object information. However, some technical requirements should be taken into account. The imaging geometry for both sensors should be nearly identical, the geometrical resolution and the wavelength should be similar and at best the imaging process should be carried out simultaneously. Therefore theoretical and experimental investigations on a suitable combination of MMW radar and radiometer information have been conducted. First experiments in 2016 have been done with an imaging linescanner based on a cylindrical imaging geometry [1]. It combines a horizontal line scan in azimuth with a linear motion in vertical direction for the second image dimension. The main drawback of the system is the limited number of pixel in vertical dimension at a certain distance. Nevertheless the near range imaging results where promising. Therefore the combination of radar and radiometer sensor was assembled on the DLR wide-field-of-view linescanner ABOSCA which is based on a spherical imaging geometry [2]. A comparison of both imaging systems is discussed. The investigations concentrate on rather basic scenarios with canonical targets like flat plates, spheres, corner reflectors and cylinders. First

  9. First TSI observations of the new Compact Lightweight Absolute Radiometer (CLARA)

    Science.gov (United States)

    Walter, B.; Finsterle, W.; Koller, S.; Levesque, P. L.; Pfiffner, D.; Schmutz, W. K.

    2017-12-01

    Continuous and precise Total Solar Irradiance (TSI) measurements are indispensable to evaluate the influence of short- and long-term solar radiative emission variations on the Earth's energy budget. The existence of a potentially long-term trend in the suns activity and whether or not such a trend could be climate effective is still a matter of debate. The Compact Lightweight Absolute Radiometer (CLARA) is one of PMOD/WRC's future contributions to the almost seamless series of space borne TSI measurements since 1978. CLARA is one of three payloads of the Norwegian micro satellite NORSAT-1, along with Langmuir probes for space plasma research and an Automatic Identification System (AIS) receiver to monitor maritime traffic in Norwegian waters. NORSAT-1 was launched July 14th 2017 and the nominal operation of CLARA will start after the instrument commissioning beginning August 21st2017. We present the design, calibration and first TSI observations of CLARA, a new generation of active cavity Electrical Substitution Radiometers (ESR) comprising the latest radiometer developments of PMOD/WRC: i) A three-cavity design for degradation tracking and redundancy, ii) a digital control loop with feed forward system allowing for measurement cadences of 30s, iii) an aperture arrangement to reduce internal scattered light and iv) a new cavity and heatsink design to minimize non-equivalence, size and weight of the instrument. CLARA was end-to-end calibrated against the SI traceable cryogenic radiometer of the TSI Radiometer Facility (TRF) in Boulder (Colorado). The absolute measurement uncertainties for the three SI-traceable TSI detectors of CLARA are 567, 576 and 912 ppm (k = 1).

  10. Preliminary Evaluation of the SMAP Radiometer Soil Moisture Product over China Using In Situ Data

    Directory of Open Access Journals (Sweden)

    Yayong Sun

    2017-03-01

    Full Text Available The Soil Moisture Active Passive (SMAP satellite makes coincident global measurements of soil moisture using an L-band radar instrument and an L-band radiometer. It is crucial to evaluate the errors in the newest L-band SMAP satellite-derived soil moisture products, before they are routinely used in scientific research and applications. This study represents the first evaluation of the SMAP radiometer soil moisture product over China. In this paper, a preliminary evaluation was performed using sparse in situ measurements from 655 China Meteorological Administration (CMA monitoring stations between 1 April 2015 and 31 August 2016. The SMAP radiometer-derived soil moisture product was evaluated against two schemes of original soil moisture and the soil moisture anomaly in different geographical zones and land cover types. Four performance metrics, i.e., bias, root mean square error (RMSE, unbiased root mean square error (ubRMSE, and the correlation coefficient (R, were used in the accuracy evaluation. The results indicated that the SMAP radiometer-derived soil moisture product agreed relatively well with the in situ measurements, with ubRMSE values of 0.058 cm3·cm−3 and 0.039 cm3·cm−3 based on original data and anomaly data, respectively. The values of the SMAP radiometer-based soil moisture product were overestimated in wet areas, especially in the Southwest China, South China, Southeast China, East China, and Central China zones. The accuracies over croplands and in Northeast China were the worst. Soil moisture, surface roughness, and vegetation are crucial factors contributing to the error in the soil moisture product. Moreover, radio frequency interference contributes to the overestimation over the northern portion of the East China zone. This study provides guidelines for the application of the SMAP-derived soil moisture product in China and acts as a reference for improving the retrieval algorithm.

  11. Preparation and quality control of radiometal-DOTARituximab

    International Nuclear Information System (INIS)

    Jalilian, A.R.; Kamali-dehghan, M.; Moradkhani, S.; Saddadi, F.; Mirsadeghi, L.

    2008-01-01

    In this work Rituximab has been labeled by Ga-67 using a new one-step method for in-situ preparation of macrocyclic bifunctional chelating agent, N-succinimidyl-1,4,7,10- tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTANHS). DOTA-NHS was prepared at 25 deg. C using DOTA, Nhydroxy succinimide (NHS) in CH Cl in one step. DOTA- 2 2 Rituximab was obtained by the addition of 1 ml of a Rituximab pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=7.8) to a glass tube pre-coated with DOTA-NHS (0.01-0.1 mg) at 25 deg. C with continuous mild stirring for 15 hours. Radiolabeling was performed at 37 deg. C in 3 hrs using Ga-67 Chlorides or Cu-64 Acetate. Thin layer radiochromatography demonstrated an overall radiochemical purity of 90-95% at optimized conditions (specific activity =30 GBq/mg, labeling efficacy= 82%). The final isotonic radio-metal-DOTA-Rituximab complex was checked by gel electrophoresis for radiolysis. TLC was performed to ensure that only one species was present after filtration through a 0.22 μm filter. Preliminary bio-distribution studies of the Ga-67 immunoconjugate in normal rat model performed to determine complex distribution of the radioimmunoconjugate up to 28hrs by imaging and organ counting after sacrificing the rats. In this study a facile method for incorporation of metal chelating moiety into peptides and antibody structure is presented. (author)

  12. Antibody engineering: methods and protocols

    National Research Council Canada - National Science Library

    Chames, Patrick

    2012-01-01

    "Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient...

  13. What Is Antiphospholipid Antibody Syndrome?

    Science.gov (United States)

    ... Back To Health Topics / Antiphospholipid Antibody Syndrome Antiphospholipid Antibody Syndrome Also known as What Is Antiphospholipid (AN-te-fos-fo-LIP-id) antibody syndrome (APS) is an autoimmune disorder. Autoimmune disorders ...

  14. Dosimetry of radiolabeled monoclonal antibodies used for therapy

    International Nuclear Information System (INIS)

    Myers, M.J.; Hooker, G.R.; Epenetos, A.A.

    1986-01-01

    The present state of radiotherapy using labeled antibodies is reviewed. From the point of view of dosimetry, antibody therapy does not seem to have reached a stable and practicable enough state to provide an input to any but rather tentative dosimetry models. These, therefore, should not be taken too far until the problems of antibody targeting have been more fully developed. Some of the instrumental techniques for acquiring dosimetric data under clinical conditions are discussed as are some of the techniques of therapy in use today. 8 references, 3 figures

  15. Uses of stable isotopes

    International Nuclear Information System (INIS)

    Axente, Damian

    1998-01-01

    The most important fields of stable isotope use with examples are presented. These are: 1. Isotope dilution analysis: trace analysis, measurements of volumes and masses; 2. Stable isotopes as tracers: transport phenomena, environmental studies, agricultural research, authentication of products and objects, archaeometry, studies of reaction mechanisms, structure and function determination of complex biological entities, studies of metabolism, breath test for diagnostic; 3. Isotope equilibrium effects: measurement of equilibrium effects, investigation of equilibrium conditions, mechanism of drug action, study of natural processes, water cycle, temperature measurements; 4. Stable isotope for advanced nuclear reactors: uranium nitride with 15 N as nuclear fuel, 157 Gd for reactor control. In spite of some difficulties of stable isotope use, particularly related to the analytical techniques, which are slow and expensive, the number of papers reporting on this subject is steadily growing as well as the number of scientific meetings organized by International Isotope Section and IAEA, Gordon Conferences, and regional meeting in Germany, France, etc. Stable isotope application development on large scale is determined by improving their production technologies as well as those of labeled compound and the analytical techniques. (author)

  16. Calcium stable isotope geochemistry

    Energy Technology Data Exchange (ETDEWEB)

    Gausonne, Nikolaus [Muenster Univ. (Germany). Inst. fuer Mineralogie; Schmitt, Anne-Desiree [Strasbourg Univ. (France). LHyGeS/EOST; Heuser, Alexander [Bonn Univ. (Germany). Steinmann-Inst. fuer Geologie, Mineralogie und Palaeontologie; Wombacher, Frank [Koeln Univ. (Germany). Inst. fuer Geologie und Mineralogie; Dietzel, Martin [Technische Univ. Graz (Austria). Inst. fuer Angewandte Geowissenschaften; Tipper, Edward [Cambridge Univ. (United Kingdom). Dept. of Earth Sciences; Schiller, Martin [Copenhagen Univ. (Denmark). Natural History Museum of Denmark

    2016-08-01

    This book provides an overview of the fundamentals and reference values for Ca stable isotope research, as well as current analytical methodologies including detailed instructions for sample preparation and isotope analysis. As such, it introduces readers to the different fields of application, including low-temperature mineral precipitation and biomineralisation, Earth surface processes and global cycling, high-temperature processes and cosmochemistry, and lastly human studies and biomedical applications. The current state of the art in these major areas is discussed, and open questions and possible future directions are identified. In terms of its depth and coverage, the current work extends and complements the previous reviews of Ca stable isotope geochemistry, addressing the needs of graduate students and advanced researchers who want to familiarize themselves with Ca stable isotope research.

  17. Calcium stable isotope geochemistry

    International Nuclear Information System (INIS)

    Gausonne, Nikolaus; Schmitt, Anne-Desiree; Heuser, Alexander; Wombacher, Frank; Dietzel, Martin; Tipper, Edward; Schiller, Martin

    2016-01-01

    This book provides an overview of the fundamentals and reference values for Ca stable isotope research, as well as current analytical methodologies including detailed instructions for sample preparation and isotope analysis. As such, it introduces readers to the different fields of application, including low-temperature mineral precipitation and biomineralisation, Earth surface processes and global cycling, high-temperature processes and cosmochemistry, and lastly human studies and biomedical applications. The current state of the art in these major areas is discussed, and open questions and possible future directions are identified. In terms of its depth and coverage, the current work extends and complements the previous reviews of Ca stable isotope geochemistry, addressing the needs of graduate students and advanced researchers who want to familiarize themselves with Ca stable isotope research.

  18. Precipitation Estimation Using Combined Radar/Radiometer Measurements Within the GPM Framework

    Science.gov (United States)

    Hou, Arthur

    2012-01-01

    The Global Precipitation Measurement (GPM) Mission is an international satellite mission specifically designed to unify and advance precipitation measurements from a constellation of research and operational microwave sensors. The GPM mission centers upon the deployment of a Core Observatory in a 65o non-Sun-synchronous orbit to serve as a physics observatory and a transfer standard for intersatellite calibration of constellation radiometers. The GPM Core Observatory will carry a Ku/Ka-band Dual-frequency Precipitation Radar (DPR) and a conical-scanning multi-channel (10-183 GHz) GPM Microwave Radiometer (GMI). The DPR will be the first dual-frequency radar in space to provide not only measurements of 3-D precipitation structures but also quantitative information on microphysical properties of precipitating particles needed for improving precipitation retrievals from microwave sensors. The DPR and GMI measurements will together provide a database that relates vertical hydrometeor profiles to multi-frequency microwave radiances over a variety of environmental conditions across the globe. This combined database will be used as a common transfer standard for improving the accuracy and consistency of precipitation retrievals from all constellation radiometers. For global coverage, GPM relies on existing satellite programs and new mission opportunities from a consortium of partners through bilateral agreements with either NASA or JAXA. Each constellation member may have its unique scientific or operational objectives but contributes microwave observations to GPM for the generation and dissemination of unified global precipitation data products. In addition to the DPR and GMI on the Core Observatory, the baseline GPM constellation consists of the following sensors: (1) Special Sensor Microwave Imager/Sounder (SSMIS) instruments on the U.S. Defense Meteorological Satellite Program (DMSP) satellites, (2) the Advanced Microwave Scanning Radiometer-2 (AMSR-2) on the GCOM-W1

  19. Radiolabelled antibodies in imaging

    International Nuclear Information System (INIS)

    Khaw, B.A.; Haber, E.

    1982-01-01

    Recent technological advances make it possible to produce pure (monoclonal) antibodies in unlimited quantities without the need for continuous immunization of animals and to label these antibodies with a variety of radionuclides which can be traced by single-photon computed tomography. An outline review of the state of the art is presented, with particular reference to the imaging of myocardial infarcts and to tumour imaging studies using labelled monoclonal antibodies (sup(99m)Tc and 125 I). Lengthy bibliography. (U.K.)

  20. A generalized quantitative antibody homeostasis model: maintenance of global antibody equilibrium by effector functions.

    Science.gov (United States)

    Prechl, József

    2017-11-01

    The homeostasis of antibodies can be characterized as a balanced production, target-binding and receptor-mediated elimination regulated by an interaction network, which controls B-cell development and selection. Recently, we proposed a quantitative model to describe how the concentration and affinity of interacting partners generates a network. Here we argue that this physical, quantitative approach can be extended for the interpretation of effector functions of antibodies. We define global antibody equilibrium as the zone of molar equivalence of free antibody, free antigen and immune complex concentrations and of dissociation constant of apparent affinity: [Ab]=[Ag]=[AbAg]= K D . This zone corresponds to the biologically relevant K D range of reversible interactions. We show that thermodynamic and kinetic properties of antibody-antigen interactions correlate with immunological functions. The formation of stable, long-lived immune complexes correspond to a decrease of entropy and is a prerequisite for the generation of higher-order complexes. As the energy of formation of complexes increases, we observe a gradual shift from silent clearance to inflammatory reactions. These rules can also be applied to complement activation-related immune effector processes, linking the physicochemical principles of innate and adaptive humoral responses. Affinity of the receptors mediating effector functions shows a wide range of affinities, allowing the continuous sampling of antibody-bound antigen over the complete range of concentrations. The generation of multivalent, multicomponent complexes triggers effector functions by crosslinking these receptors on effector cells with increasing enzymatic degradation potential. Thus, antibody homeostasis is a thermodynamic system with complex network properties, nested into the host organism by proper immunoregulatory and effector pathways. Maintenance of global antibody equilibrium is achieved by innate qualitative signals modulating a

  1. Development of a Compact High Altitude Imager and Sounding Radiometer (CHAISR)

    Science.gov (United States)

    Choi, R. K. Y.; Min, S.; Cho, Y. J.; Kim, K. H.; Ha, J. C.; Joo, S. W.

    2017-12-01

    Joint Civilian-Military Committee, under Advisory Council on Science and Technology, Korea, has approved a technology demonstration project for developing a lightweight HALE UAV (High-Altitude, Long Endurance). It aims to operate at lower stratosphere, i.e. altitude of 16 20 km, offering unique observational platform to atmospheric research community as pseudo-satellite. NIMS (National Institute of Meteorological Sciences, Korea) is responsible for a payload for atmospheric science, a Compact High Altitude Imager and Sounding Radiometer (CHAISR) to demonstrate scientific observations at lower stratosphere in the interest of improving numerical weather prediction model. CHAISR consists of three microwave radiometers (MWR) with 16 channel, and medium resolution cameras operating in a visible and infrared spectrum. One of the technological challenges for CHAISR is to accommodate those instruments within 50 W of power consumption. CHAISR will experience temperature up to -75°C, while pressure as low as 50 hPa at operational altitude. It requires passive thermal control of the payload to keep electronic subsystems warm enough for instrument operation with minimal power available. Safety features, such as payload power management and thermal control, are considered with minimal user input. Three radiometers measure atmospheric brightness temperature at frequency at around 20, 40, and 50 GHz. Retrieval process yields temperature and humidity profiles with cross track scan along the flight line. Estimated total weight of all radiometer hardware, from the antennas to data acquisition system, is less than 0.8 kg and a maximum power consumption is 15.2 W. With not enough power for blackbody calibration target, radiometers use zenith sky view at lower stratosphere as an excellent calibration target for a conventional tipping-curve calibration. Spatial distributions of clouds from visible and surface temperature from thermal cameras are used as additional information for

  2. A Radar/Radiometer Instrument for Mapping Soil Moisture and Ocean Salinity

    Science.gov (United States)

    Hildebrand, Peter H.; Hilliard, Laurence; Rincon, Rafael; LeVine, David; Mead, James

    2003-01-01

    The RadSTAR instrument combines an L-band, digital beam-forming radar with an L-band synthetic aperture, thinned array (STAR) radiometer. The RadSTAR development will support NASA Earth science goals by developing a novel, L-band scatterometer/ radiometer that measures Earth surface bulk material properties (surface emissions and backscatter) as well as surface characteristics (backscatter). Present, real aperture airborne L-Band active/passive measurement systems such as the JPUPALS (Wilson, et al, 2000) provide excellent sampling characteristics, but have no scanning capabilities, and are extremely large; the huge JPUPALS horn requires a the C-130 airborne platform, operated with the aft loading door open during flight operation. The approach used for the upcoming Aquarius ocean salinity mission or the proposed Hydros soil mission use real apertures with multiple fixed beams or scanning beams. For real aperture instruments, there is no upgrade path to scanning over a broad swath, except rotation of the whole aperture, which is an approach with obvious difficulties as aperture size increases. RadSTAR will provide polarimetric scatterometer and radiometer measurements over a wide swath, in a highly space-efficient configuration. The electronic scanning approaches provided through STAR technology and digital beam forming will enable the large L-band aperture to scan efficiently over a very wide swath. RadSTAR technology development, which merges an interferometric radiometer with a digital beam forming scatterometer, is an important step in the path to space for an L-band scatterometer/radiometer. RadSTAR couples a patch array antenna with a 1.26 GHz digital beam forming radar scatterometer and a 1.4 GHz STAR radiometer to provide Earth surface backscatter and emission measurements in a compact, cross-track scanning instrument with no moving parts. This technology will provide the first L-band, emission and backscatter measurements in a compact aircraft instrument

  3. {sup 68}Ga-labelled recombinant antibody variants for immuno-PET imaging of solid tumours

    Energy Technology Data Exchange (ETDEWEB)

    Eder, Matthias; Eisenhut, Michael [German Cancer Research Center, Radiopharmaceutical Chemistry, Heidelberg (Germany); Knackmuss, Stefan; Gall, Fabrice Le; Reusch, Uwe; Little, Melvyn [Affimed Therapeutics AG, Heidelberg (Germany); Rybin, Vladimir [European Molecular Biology Laboratory, Heidelberg (Germany); Haberkorn, Uwe; Mier, Walter [University of Heidelberg, Department of Nuclear Medicine, Heidelberg (Germany)

    2010-07-15

    Recombinant antibodies isolated from human antibody libraries have excellent affinities and high target specificity. As full-length IgGs are cleared inadequately slowly from the circulation, the aim of this work was to figure out which kind of recombinant antibody fragment proves to be appropriate for imaging epithelial cell adhesion molecule (EpCAM)-expressing tumours with the short-living radioisotope {sup 68}Ga. In order to combine the promising tumour targeting properties of antibodies with {sup 68}Ga, four antibody variants with the same specificity and origin only differing in molecular weight were constructed for comparison. Therefore, the binding domains of a single-chain fragment variable (scFv) isolated from a human naive antibody library were modified genetically to construct the respective full-length IgG, the tria- and diabody variants. These molecules were conjugated with the bifunctional chelating agent N,N{sup '}-bis[2-hydroxy-5-(carboxyethyl)benzyl]ethylenediamine-N,N{sup '}-diacetic acid (HBED-CC) to enable {sup 68}Ga labelling at ambient temperature and compared in biodistribution and immuno-PET imaging experiments. The antibody variants with identical specificity proved to have the correct molecular weight, high binding affinity and specificity to their antigen, EpCAM. Radiometal complexation was efficiently performed at room temperature leading to {sup 68}Ga-labelled antibodies with unchanged binding properties compared to the original antibody variants. The best targeting properties were obtained with the scFv and especially with the diabody. The triabody showed higher absolute tumour uptake but only moderate clearance from circulation. The antibody variants differed considerably in normal organ uptake, clearance from circulation and tumour accumulation. The data demonstrate the feasibility of imaging solid tumours with the {sup 68}Ga-labelled diabody format. This type of recombinant protein might be a promising carrier even for the

  4. Monoclonal antibodies in oncology

    International Nuclear Information System (INIS)

    Chan, S.Y.T.; Sikora, K.

    1986-01-01

    Monoclonal antibodies (MCAs) can be used to differentiate between normal and neoplastic cells and thus exploited for diagnostic and, ultimately, therapeutic gain. The evidence for the existence of human tumour antigens is reviewed. Several areas of diagnosis are already benefiting from the application of the monoclonal technology. Immunohistology can help the pathologist with difficult diagnostic problems. New classifications of lymphoma and leukaemia can be based on specific surface molecules. Similarly, the detection of shed tumour antigens is already established as part of the routine assessment of many patients with common solid tumours. Isotopically labeled monoclonal antibodies have been used to localise primary and metastatic tumours. The use of antibodies in this way is not only a promising diagnostic tool but also the first step in studying the possibility of arming antibodies to provide therapeutic agents. Such trials are currently in progress. (Auth.)

  5. Future of antibody purification.

    Science.gov (United States)

    Low, Duncan; O'Leary, Rhona; Pujar, Narahari S

    2007-03-15

    Antibody purification seems to be safely ensconced in a platform, now well-established by way of multiple commercialized antibody processes. However, natural evolution compels us to peer into the future. This is driven not only by a large, projected increase in the number of antibody therapies, but also by dramatic improvements in upstream productivity, and process economics. Although disruptive technologies have yet escaped downstream processes, evolution of the so-called platform is already evident in antibody processes in late-stage development. Here we perform a wide survey of technologies that are competing to be part of that platform, and provide our [inherently dangerous] assessment of those that have the most promise.

  6. Serum herpes simplex antibodies

    Science.gov (United States)

    ... causes cold sores (oral herpes). HSV-2 causes genital herpes. How the Test is Performed A blood sample ... person has ever been infected with oral or genital herpes . It looks for antibodies to herpes simplex virus ...

  7. Stable isotope studies

    International Nuclear Information System (INIS)

    Ishida, T.

    1992-01-01

    The research has been in four general areas: (1) correlation of isotope effects with molecular forces and molecular structures, (2) correlation of zero-point energy and its isotope effects with molecular structure and molecular forces, (3) vapor pressure isotope effects, and (4) fractionation of stable isotopes. 73 refs, 38 figs, 29 tabs

  8. Interactive Stable Ray Tracing

    DEFF Research Database (Denmark)

    Dal Corso, Alessandro; Salvi, Marco; Kolb, Craig

    2017-01-01

    Interactive ray tracing applications running on commodity hardware can suffer from objectionable temporal artifacts due to a low sample count. We introduce stable ray tracing, a technique that improves temporal stability without the over-blurring and ghosting artifacts typical of temporal post-pr...

  9. The Stable Concordance Genus

    OpenAIRE

    Kearney, M. Kate

    2013-01-01

    The concordance genus of a knot is the least genus of any knot in its concordance class. Although difficult to compute, it is a useful invariant that highlights the distinction between the three-genus and four-genus. In this paper we define and discuss the stable concordance genus of a knot, which describes the behavior of the concordance genus under connected sum.

  10. Stable radiographic scanning agents

    International Nuclear Information System (INIS)

    1976-01-01

    Stable compositions which are useful in the preparation of Technetium-99m-based scintigraphic agents are discussed. They are comprised of ascorbic acid or a pharmaceutically acceptable salt or ester thereof in combination with a pertechnetate reducing agent or dissolved in oxidized pertechnetate-99m (sup(99m)TcO 4 - ) solution

  11. Some stable hydromagnetic equilibria

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, J L; Oberman, C R; Kulsrud, R M; Frieman, E A [Project Matterhorn, Princeton University, Princeton, NJ (United States)

    1958-07-01

    We have been able to find and investigate the properties of equilibria which are hydromagnetically stable. These equilibria can be obtained, for example, by wrapping conductors helically around the stellarator tube. Systems with I = 3 or 4 are indicated to be optimum for stability purposes. In some cases an admixture of I = 2 fields can be advantageous for achieving equilibrium. (author)

  12. Development of an Internally-Calibrated Wide-Band Airborne Microwave Radiometer to Provide High-Resolution Wet-Tropospheric Path Delay Measurements for SWOT (HAMMR - High-frequency Airborne Microwave and Millimeter-wave Radiometer)

    Data.gov (United States)

    National Aeronautics and Space Administration — Development of an Internally-Calibrated Wide-Band Airborne Microwave Radiometer to Provide High-Resolution Wet-Tropospheric Path Delay Measurements for SWOT (HAMMR -...

  13. Antibody tumor penetration

    Science.gov (United States)

    Thurber, Greg M.; Schmidt, Michael M.; Wittrup, K. Dane

    2009-01-01

    Antibodies have proven to be effective agents in cancer imaging and therapy. One of the major challenges still facing the field is the heterogeneous distribution of these agents in tumors when administered systemically. Large regions of untargeted cells can therefore escape therapy and potentially select for more resistant cells. We present here a summary of theoretical and experimental approaches to analyze and improve antibody penetration in tumor tissue. PMID:18541331

  14. Towards tumour targeting with copper-radiolabelled macrocycle-antibody conjugates

    International Nuclear Information System (INIS)

    Morphy, J.R.; Parker, David; Kataky, Ritu

    1989-01-01

    Tetraaza-macrocycles covalently attached to a monoclonal antibody may be efficiently radiolabelled with 64 Cu or 67 Cu at pH4, minimising non-specific binding to the protein, giving a kinetically stable conjugate in vivo. (author)

  15. Ozone, spectral irradiance and aerosol measurements with the Brewer spectro radiometer

    International Nuclear Information System (INIS)

    Marenco, F.; Di Sarra, A.

    2001-01-01

    In this technical report a detailed description of the Brewer spectro radiometer, a widespread instrument for ozone and ultraviolet radiation, is given. The methodologies used to measure these quantities and for instrument calibration are described in detail. Finally a new methodology, developed by ENEA to derive the aerosol optical depth from the Brewer routine total ozone measurements, is described. This methodology is based on Langley extrapolation, on the determination of the transmissivity of the Brewer neutral density filters, and on a statistically significant number of half days of measurements obtained in could-free conditions. Results of this method, obtained with the Brewer of the ENEA station for climate observations Roberto Sarao, located in the island of Lampedusa, are reported. These results confirm the validity of the method, thanks to independent measurements taken in 1999 with a Multi filter Rotating Shadow band Radiometer. This methodology allows researchers to obtain an aerosol climatology from ozone measurements obtained at several sites world-wide [it

  16. Measurement of synchrotron radiation from the NBS SURF II using a silicon radiometer

    International Nuclear Information System (INIS)

    Schaefer, A.R.

    1980-01-01

    A project is described in which the synchrotron radiation output from the NBS storage ring known as SURF II, is measured using a well characterized silicon based radiometer. This device consists of a silicon photodiode coupled with two interference filters to restrict the spectral response to a finite and convenient spectral region for the measurement. Considerations required for the characterization of the radiometer will be discussed. The absolute radiant flux from the storage ring is also calculable from various machine parameters. A measurement of the number of circulating electrons will be derived from electron counting techniques at low levels. This will yield an important intercomparison between the synchrotron flux measurements determined in two entirely different ways. (orig.)

  17. Design and first plasma measurements of the ITER-ECE prototype radiometer

    Energy Technology Data Exchange (ETDEWEB)

    Austin, M. E.; Brookman, M. W.; Rowan, W. L. [Institute for Fusion Studies, University of Texas at Austin, Austin, Texas 78712 (United States); Danani, S. [ITER-India/Institute for Plasma Research, Bhat, Gandhinagar 382428 (India); Bryerton, E. W.; Dougherty, P. [Virginia Diodes, Inc., Charlottesville, Virginia 22902 (United States)

    2016-11-15

    On ITER, second harmonic optically thick electron cyclotron emission (ECE) in the range of 220-340 GHz will supply the electron temperature (T{sub e}). To investigate the requirements and capabilities prescribed for the ITER system, a prototype radiometer covering this frequency range has been developed by Virginia Diodes, Inc. The first plasma measurements with this instrument have been carried out on the DIII-D tokamak, with lab bench tests and measurements of third through fifth harmonic ECE from high T{sub e} plasmas. At DIII-D the instrument shares the transmission line of the Michelson interferometer and can simultaneously acquire data. Comparison of the ECE radiation temperature from the absolutely calibrated Michelson and the prototype receiver shows that the ITER radiometer provides accurate measurements of the millimeter radiation across the instrument band.

  18. The along track scanning radiometer - an analysis of coincident ship and satellite measurements

    Science.gov (United States)

    Barton, I. J.; Prata, A. J.; Llewellyn-Jones, D. T.

    1993-05-01

    Following the successful launch of the ERS-1 satellite in July 1991 we have undertaken several geophysical validation cruises in the Coral Sea. The prime aim of these cruises was to compare the sea surface temperature (SST) derived from the Along Track Scanning Radiometer (ATSR) with that measured using precision radiometers mounted on the ships. On most occasions when simultaneous satellite and ship measurements were taken we also launched a radiosonde from one of the research vessels. The results suggest that the ATSR is able to measure the ``skin'' temperature of the sea surface with an accuracy suitable for climate research applications. A case study comparison between the AVHRR and ATSR SST products will also be presented.

  19. Nimbus-2 High-Resolution Infrared Radiometer (HRIR) Imagery of Cloud Cover at Night on 70 mm Film V001

    Data.gov (United States)

    National Aeronautics and Space Administration — The HRIRN2IM data product contains scanned negatives of photofacsimile 70mm film strips from the Nimbus-2 High-Resolution Infrared Radiometer. The images contain...

  20. Wide-band Millimeter and Sub-Millimeter Wave Radiometer Instrument to Measure Tropospheric Water and Cloud ICE

    Data.gov (United States)

    National Aeronautics and Space Administration — We propose to develop, fabricate and test a new, multi-frequency millimeter and sub-millimeter-wave radiometer instrument to provide critically-needed measurements...

  1. NPP Visible Infrared Imager-Radiometer Suite (VIIRS) Diffuse Attenuation Coefficient for Downwelling Irradiance (KD) Global Mapped Data

    Data.gov (United States)

    National Aeronautics and Space Administration — The Visible and Infrared Imager/Radiometer Suite (VIIRS) is a multi-disciplinary instrument that is being flown on the Joint Polar Satellite System (JPSS) series of...

  2. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Near Constant Contrast (NCC) Imagery Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument onboard the...

  3. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Base Height (CBH) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Cloud Base Heights (CBH) from the Visible Infrared Imaging Radiometer Suite...

  4. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Type and Phase Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of cloud type and phase from the Visible Infrared Imaging Radiometer Suite (VIIRS)...

  5. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Land Surface Temperature (LST) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Land Surface Temperature (LST) from the Visible Infrared Imaging Radiometer Suite...

  6. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Cover Layer (CCL) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality Environmental Data Record (EDR) of Cloud Cover Layers (CCL) from the Visible Infrared Imaging Radiometer Suite (VIIRS)...

  7. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Optical Thickness (COT) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Cloud Optical Thickness (COT) from the Visible Infrared Imaging Radiometer Suite...

  8. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Ice Thickness and Age Environmental Data Records (EDRs) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Ice Thickness and Age from the Visible Infrared Imaging Radiometer Suite (VIIRS)...

  9. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Ice Surface Temperature (IST) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument onboard the...

  10. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Top Height (CTH) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument onboard the...

  11. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Top Temperature (CTT) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument onboard the...

  12. A precise narrow-beam filter infrared radiometer and its use with lidar in the ARM Program

    International Nuclear Information System (INIS)

    Platt, C.M.R.

    1992-05-01

    The first six months of the grant (December 1991--May 1992) have been taken up with the design and specification for the new narrow-beam radiometer. The radiometer will be built and tested at the Division of Atmospheric Research over the next three months. Improved algorithms for obtaining cloud extinction have also been developed. It is proposed during 1993 to use the radiometer in conjunction with a new CSIRO 3-wavelength lidar in the ARM PROBE experiment at Kavieng, New Guinea, which is a test mission under tropical conditions for the ARM CART Tropical West Pacific site, and is part of the TOGA COARE experiment. During the latter part of 1992, the radiometer will be tested thoroughly and tested at the Division of Atmospheric Research, Aspendale

  13. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Effective Particle Size (CEPS) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Cloud Effective Particle Size (CEPS) from the Visible Infrared Imaging Radiometer...

  14. JPSS NOAA Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Top Pressure (CTP) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument onboard the...

  15. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Sea Ice Characterization (SIC) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains an Environmental Data Record (EDR) of Sea Ice Characterization (SIC) from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument...

  16. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Height (Top and Base) Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of cloud height (top and base) from the Visible Infrared Imaging Radiometer Suite...

  17. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Ocean Color/Chlorophyll (OCC) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Ocean Color/Chlorophyll (OCC) from the Visible Infrared Imaging Radiometer Suite...

  18. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Volcanic Ash Detection and Height Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of volcanic ash from the Visible Infrared Imaging Radiometer (VIIRS) instrument...

  19. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Imagery (not Near Constant Contrast) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument onboard the...

  20. HURRICANE AND SEVERE STORM SENTINEL (HS3) GLOBAL HAWK HIGH ALTITUDE MMIC SOUNDING RADIOMETER (HAMSR) V1

    Data.gov (United States)

    National Aeronautics and Space Administration — The Hurricane and Severe Storm Sentinel (HS3) Global Hawk High Altitude MMIC Sounding Radiometer (HAMSR) datasets include measurements gathered by the HAMSR...

  1. Assimilation of global radar backscatter and radiometer brightness temperature observations to improve soil moisture and land evaporation estimates

    NARCIS (Netherlands)

    Lievens, H.; Martens, B.; Verhoest, N.E.C.; Hahn, S.; Reichle, R.H.; Gonzalez Miralles, D.

    2016-01-01

    Active radar backscatter (σ°) observations from the Advanced Scatterometer (ASCAT) and passive radiometer brightness temperature (TB) observations from the Soil Moisture Ocean Salinity (SMOS) mission are assimilated either individually or jointly into the Global Land Evaporation Amsterdam Model

  2. Weight estimates and packaging techniques for the microwave radiometer spacecraft. [shuttle compatible design

    Science.gov (United States)

    Jensen, J. K.; Wright, R. L.

    1981-01-01

    Estimates of total spacecraft weight and packaging options were made for three conceptual designs of a microwave radiometer spacecraft. Erectable structures were found to be slightly lighter than deployable structures but could be packaged in one-tenth the volume. The tension rim concept, an unconventional design approach, was found to be the lightest and transportable to orbit in the least number of shuttle flights.

  3. Global Land Surface Temperature From the Along-Track Scanning Radiometers

    Science.gov (United States)

    Ghent, D. J.; Corlett, G. K.; Göttsche, F.-M.; Remedios, J. J.

    2017-11-01

    The Leicester Along-Track Scanning Radiometer (ATSR) and Sea and Land Surface Temperature Radiometer (SLSTR) Processor for LAnd Surface Temperature (LASPLAST) provides global land surface temperature (LST) products from thermal infrared radiance data. In this paper, the state-of-the-art version of LASPLAST, as deployed in the GlobTemperature project, is described and applied to data from the Advanced Along-Track Scanning Radiometer (AATSR). The LASPLAST retrieval formulation for LST is a nadir-only, two-channel, split-window algorithm, based on biome classification, fractional vegetation, and across-track water vapor dependences. It incorporates globally robust retrieval coefficients derived using highly sampled atmosphere profiles. LASPLAST benefits from appropriate spatial resolution auxiliary information and a new probabilistic-based cloud flagging algorithm. For the first time for a satellite-derived LST product, pixel-level uncertainties characterized in terms of random, locally correlated, and systematic components are provided. The new GlobTemperature GT_ATS_2P Version 1.0 product has been validated for 1 year of AATSR data (2009) against in situ measurements acquired from "gold standard reference" stations: Gobabeb, Namibia, and Evora, Portugal; seven Surface Radiation Budget stations, and the Atmospheric Radiation Measurement station at Southern Great Plains. These data show average absolute biases for the GT_ATS_2P Version 1.0 product of 1.00 K in the daytime and 1.08 K in the nighttime. The improvements in data provenance including better accuracy, fully traceable retrieval coefficients, quantified uncertainty, and more detailed information in the new harmonized format of the GT_ATS_2P product will allow for more significant exploitation of the historical LST data record from the ATSRs and a valuable near-real-time service from the Sea and Land Surface Temperature Radiometers (SLSTRs).

  4. Microfluidic labeling of biomolecules with radiometals for use in nuclear medicine.

    Science.gov (United States)

    Wheeler, Tobias D; Zeng, Dexing; Desai, Amit V; Önal, Birce; Reichert, David E; Kenis, Paul J A

    2010-12-21

    Radiometal-based radiopharmaceuticals, used as imaging and therapeutic agents in nuclear medicine, consist of a radiometal that is bound to a targeting biomolecule (BM) using a bifunctional chelator (BFC). Conventional, macroscale radiolabeling methods use an excess of the BFC-BM conjugate (ligand) to achieve high radiolabeling yields. Subsequently, to achieve maximal specific activity (minimal amount of unlabeled ligand), extensive chromatographic purification is required to remove unlabeled ligand, often resulting in longer synthesis times and loss of imaging sensitivity due to radioactive decay. Here we describe a microreactor that overcomes the above issues through integration of efficient mixing and heating strategies while working with small volumes of concentrated reagents. As a model reaction, we radiolabel 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) conjugated to the peptide cyclo(Arg-Gly-Asp-DPhe-Lys) with (64)Cu(2+). We show that the microreactor (made from polydimethylsiloxane and glass) can withstand 260 mCi of activity over 720 hours and retains only minimal amounts of (64)Cu(2+) (50 µM), yields of over 90% can be achieved in the microreactor when using a 1:1 stoichiometry of radiometal to BFC-BM. These high yields eliminate the need for use of excess amounts of often precious BM and obviate the need for a chromatographic purification process to remove unlabeled ligand. The results reported here demonstrate the potential of microreactor technology to improve the production of patient-tailored doses of radiometal-based radiopharmaceuticals in the clinic.

  5. A General Analysis of the Impact of Digitization in Microwave Correlation Radiometers

    Directory of Open Access Journals (Sweden)

    Hyuk Park

    2011-06-01

    Full Text Available This study provides a general framework to analyze the effects on correlation radiometers of a generic quantization scheme and sampling process. It reviews, unifies and expands several previous works that focused on these effects separately. In addition, it provides a general theoretical background that allows analyzing any digitization scheme including any number of quantization levels, irregular quantization steps, gain compression, clipping, jitter and skew effects of the sampling period.

  6. Atmospheric water distribution in cyclones as seen with Scanning Multichannel Microwave Radiometers (SMMR)

    Science.gov (United States)

    Katsaros, K. B.; Mcmurdie, L. A.

    1983-01-01

    Passive microwave measurements are used to study the distribution of atmospheric water in midlatitude cyclones. The integrated water vapor, integrated liquid water, and rainfall rate are deduced from the brightness temperatures at microwave frequencies measured by the Scanning Multichannel Microwave Radiometer (SMRR) flown on both the Seasat and Nimbus 7 satellites. The practical application of locating fronts by the cyclone moisture pattern over oceans is shown, and the relationship between the quantity of coastal rainfall and atmospheric water content is explored.

  7. Absolute intensity calibration of the 32-channel heterodyne radiometer on experimental advanced superconducting tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Liu, X.; Zhao, H. L.; Liu, Y., E-mail: liuyong@ipp.ac.cn; Li, E. Z.; Han, X.; Ti, A.; Hu, L. Q.; Zhang, X. D. [Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031 (China); Domier, C. W.; Luhmann, N. C. [Department of Electrical and Computer Engineering, University of California at Davis, Davis, California 95616 (United States)

    2014-09-15

    This paper presents the results of the in situ absolute intensity calibration for the 32-channel heterodyne radiometer on the experimental advanced superconducting tokamak. The hot/cold load method is adopted, and the coherent averaging technique is employed to improve the signal to noise ratio. Measured spectra and electron temperature profiles are compared with those from an independent calibrated Michelson interferometer, and there is a relatively good agreement between the results from the two different systems.

  8. A radiative transfer model for sea surface temperature retrieval for the along-track scanning radiometer

    Science.gov (United States)

    ZáVody, A. M.; Mutlow, C. T.; Llewellyn-Jones, D. T.

    1995-01-01

    The measurements made by the along-track scanning radiometer are now converted routinely into sea surface temperature (SST). The details of the atmospheric model which had been used for deriving the SST algorithms are given, together with tables of the coefficients in the algorithms for the different SST products. The accuracy of the retrieval under normal conditions and the effect of errors in the model on the retrieved SST are briefly discussed.

  9. What is wrong with radioimmunodetection and radioimmunotherapy of tumours with labelled monoclonal antibodies or with radionuclides

    International Nuclear Information System (INIS)

    Shukla, S.K.; Cipriani, C.; Manni, G.B.

    1986-01-01

    Chromatographically pure and stable anionic species of high charge density cations show higher uptake and give better melanoma images than monoclonal antibodies labeled with the same radionulcides. sup(99m)Tc-labeled antimelanoma antibody filtered for the removal of heavy colloids, hydroxides and oxides of 99m-technetium shows lower uptake in the liver and spleen. (Author)

  10. CORRECTION OF THE TEMPERATURE EFFECT IN 1020 NM BAND OF SUN-SKY RADIOMETER

    Directory of Open Access Journals (Sweden)

    K. Li

    2018-04-01

    Full Text Available Aerosol is an important part of the earth-atmosphere system. It can directly and indirectly influence solar radiation and then affect the energy balance of earth-atmosphere system. AERONET, as the largest ground-based observation network, provides multi-parameters of aerosol from more than 600 hundred sites using sun-sky radiometer, which contains 9 channels from 340 nm to 1640 nm. Among which, 1020 nm channel is greatly influenced by the temperature. In this paper, a new correction method of 1020 nm band is introduced. The new method transfers the temperature correction coefficient of the master radiometer to the comparative one. The filed calibration experiment shown that the temperature correction coefficient obtained by this method is close to the result from the temperature controlled chamber, and the difference is about 2.1 %. This new method is easy-to-use, and its accuracy is comparable to the standard one. It is more applicable for large-scale instrument calibration. In principle, this method is applicable to all bands of the sun-sky radiometer.

  11. High spatial resolution upgrade of the electron cyclotron emission radiometer for the DIII-D tokamak.

    Science.gov (United States)

    Truong, D D; Austin, M E

    2014-11-01

    The 40-channel DIII-D electron cyclotron emission (ECE) radiometer provides measurements of Te(r,t) at the tokamak midplane from optically thick, second harmonic X-mode emission over a frequency range of 83-130 GHz. The frequency spacing of the radiometer's channels results in a spatial resolution of ∼1-3 cm, depending on local magnetic field and electron temperature. A new high resolution subsystem has been added to the DIII-D ECE radiometer to make sub-centimeter (0.6-0.8 cm) resolution Te measurements. The high resolution subsystem branches off from the regular channels' IF bands and consists of a microwave switch to toggle between IF bands, a switched filter bank for frequency selectivity, an adjustable local oscillator and mixer for further frequency down-conversion, and a set of eight microwave filters in the 2-4 GHz range. Higher spatial resolution is achieved through the use of a narrower (200 MHz) filter bandwidth and closer spacing between the filters' center frequencies (250 MHz). This configuration allows for full coverage of the 83-130 GHz frequency range in 2 GHz bands. Depending on the local magnetic field, this translates into a "zoomed-in" analysis of a ∼2-4 cm radial region. Expected uses of these channels include mapping the spatial dependence of Alfven eigenmodes, geodesic acoustic modes, and externally applied magnetic perturbations. Initial Te measurements, which demonstrate that the desired resolution is achieved, are presented.

  12. A W-Band Radiometer with the Offset Parabolic Antenna for Radiometric Measurements

    Directory of Open Access Journals (Sweden)

    Li Wu

    2016-01-01

    Full Text Available This paper deals with the development of a W-band noise-adding radiometer which combines the millimeter-wave (MMW radiometric measurements with a high-resolution imager. The offset parabolic antenna is presented to achieve an accurate measurement and a high resolution. To reduce the cross-polarization level of the antenna, a multimode feed horn with a multistep structure is proposed to match the focal region fields of the reflector. It has advantages over the corrugated horns in lower mass and easier manufacturing. In addition, due to an unavoidable settling time for the noise-adding radiometer output signal passing through the low-pass filter, a theoretical criterion for the optimum duty cycle determination to reject extraneous contributions from the transient is proposed in this paper. The appropriate duty cycle threshold is 0.33 for the developed W-band radiometer. Also, a geometric correction method is presented to correct the obtained passive image suffering from a distortion for a better image interpretation. Preliminary experimental results are given to illustrate and verify the presented techniques.

  13. PAU-SA: A Synthetic Aperture Interferometric Radiometer Test Bed for Potential Improvements in Future Missions

    Directory of Open Access Journals (Sweden)

    Merce Vall-llosera

    2012-06-01

    Full Text Available The Soil Moisture and Ocean Salinity (SMOS mission is an Earth Explorer Opportunity mission from the European Space Agency (ESA. Its goal is to produce global maps of soil moisture and ocean salinity using the Microwave Imaging Radiometer by Aperture Synthesis (MIRAS. The purpose of the Passive Advanced Unit Synthetic Aperture (PAU-SA instrument is to study and test some potential improvements that could eventually be implemented in future missions using interferometric radiometers such as the Geoestacionary Atmosferic Sounder (GAS, the Precipitation and All-weather Temperature and Humidity (PATH and the Geostationary Interferometric Microwave Sounder (GIMS. Both MIRAS and PAU-SA are Y-shaped arrays with uniformly distributed antennas, but the receiver topology and the processing unit are quite different. The purpose of this work is to identify the elements in the MIRAS’s design susceptible of improvement and apply them in the PAU-SA instrument demonstrator, to test them in view of these future interferometric radiometer missions.

  14. Remote sensing of the lightning heating effect duration with ground-based microwave radiometer

    Science.gov (United States)

    Jiang, Sulin; Pan, Yun; Lei, Lianfa; Ma, Lina; Li, Qing; Wang, Zhenhui

    2018-06-01

    Artificially triggered lightning events from May 26, 2017 to July 16, 2017 in Guangzhou Field Experiment Site for Lightning Research and Test (GFESL) were intentionally remotely sensed with a ground-based microwave radiometer for the first time in order to obtain the features of lightning heating effect. The microwave radiometer antenna was adjusted to point at a certain elevation angle towards the expected artificially triggered lightning discharging path. Eight of the 16 successfully artificially triggered lightning events were captured and the brightness temperature data at four frequencies in K and V bands were obtained. The results from data time series analysis show that artificially triggered lightning can make the radiometer generate brightness temperature pulses, and the amplitudes of these pulses are in the range of 2.0 K to 73.8 K. The brightness temperature pulses associated with 7 events can be used to estimate the duration of lightning heating effect through accounting the number of the pulses in the continuous pulse sequence and the sampling interval between four frequencies. The maximum duration of the lightning heating effect is 1.13 s, the minimum is 0.172 s, and the average is 0.63 s.

  15. Vitamin D synthesis measured with a multiband filter radiometer in Río Gallegos, Argentina

    Science.gov (United States)

    Orte, Facundo; Wolfram, Elian; Salvador, Jacobo; D'Elia, Raúl; Bulnes, Daniela; Leme, N. Paes; Quel, Eduardo

    2013-05-01

    Vitamin D plays an important role in human health. Vitamin D production from the sun is affected by UVB solar radiation. This paper presents a simple method for retrieving vitamin D-weighted UV by using a multiband filter radiometer GUV-541 installed at the Atmospheric Observatory of Southern Patagonia (OAPA) (51 ° 33' S, 69° 19' W), Río Gallegos. The methodology used combines irradiance measurements from a multiband filter radiometer with spectral irradiance modeled by the SOS radiative transfer code (developed by Lille University of Science and Technology (USTL)). The spectrum modeled is weighted with vitamin D action spectra published by the International Commission on Illumination (CIE), which describes the relative effectiveness of different wavelengths in the generation of this particular biological response. This method is validated using the vitamin D-weighted UV derived from a Brewer MKIII spectrophotometer (SN 124) belonging to the National Institute for Spatial Research (INPE), Brazil, which is able to measure solar spectra between 290 and 325nm. The method presents a good correlation between the two independent instruments. This procedure increases the instrumental capabilities of the multiband filter radiometer. Moreover, it evaluates the annual variation of vitamin D-weighted UV doses from exposure to ultraviolet radiation. These values are likely to be lower than suitable levels of vitamin D during winter and part of spring and autumn at these latitudes.

  16. Correction of the Temperature Effect in 1020 NM Band of Sun-Sky Radiometer

    Science.gov (United States)

    Li, K.; Li, Z.; Li, D.; Xie, Y.; Xu, H.

    2018-04-01

    Aerosol is an important part of the earth-atmosphere system. It can directly and indirectly influence solar radiation and then affect the energy balance of earth-atmosphere system. AERONET, as the largest ground-based observation network, provides multi-parameters of aerosol from more than 600 hundred sites using sun-sky radiometer, which contains 9 channels from 340 nm to 1640 nm. Among which, 1020 nm channel is greatly influenced by the temperature. In this paper, a new correction method of 1020 nm band is introduced. The new method transfers the temperature correction coefficient of the master radiometer to the comparative one. The filed calibration experiment shown that the temperature correction coefficient obtained by this method is close to the result from the temperature controlled chamber, and the difference is about 2.1 %. This new method is easy-to-use, and its accuracy is comparable to the standard one. It is more applicable for large-scale instrument calibration. In principle, this method is applicable to all bands of the sun-sky radiometer.

  17. Longterm and spatial variability of Aerosol optical properties measured by sky radiometer in Japan sites

    Science.gov (United States)

    Aoki, K.

    2016-12-01

    Aerosols and cloud play an important role in the climate change. We started the long-term monitoring of aerosol and cloud optical properties since 1990's by using sky radiometer (POM-01, 02; Prede Co. Ltd., Japan). We provide the information, in this presentation, on the aerosol optical properties with respect to their temporal and spatial variability in Japan site (ex. Sapporo, Toyama, Kasuga and etc). The global distributions of aerosols have been derived from earth observation satellite and have been simulated in numerical models, which assume optical parameters. However, these distributions are difficult to derive because of variability in time and space. Therefore, Aerosol optical properties were investigated using the measurements from ground-based and ship-borne sky radiometer. The sky radiometer is an automatic instrument that takes observations only in daytime under the clear sky conditions. Observation of diffuse solar intensity interval was made every ten or five minutes by once. The aerosol optical properties were computed using the SKYRAD.pack version 4.2. The obtained Aerosol optical properties (Aerosol optical thickness, Ångström exponent, Single scattering albedo, and etc.) and size distribution volume clearly showed spatial and temporal variability in Japan area. In this study, we present the temporal and spatial variability of Aerosol optical properties at several Japan sites, applied to validation of satellite and numerical models. This project is validation satellite of GCOM-C, JAXA. The GCOM-C satellite scheduled to be launched in early 2017.

  18. Stable isotope analysis

    International Nuclear Information System (INIS)

    Tibari, Elghali; Taous, Fouad; Marah, Hamid

    2014-01-01

    This report presents results related to stable isotopes analysis carried out at the CNESTEN DASTE in Rabat (Morocco), on behalf of Senegal. These analyzes cover 127 samples. These results demonstrate that Oxygen-18 and Deuterium in water analysis were performed by infrared Laser spectroscopy using a LGR / DLT-100 with Autosampler. Also, the results are expressed in δ values (‰) relative to V-SMOW to ± 0.3 ‰ for oxygen-18 and ± 1 ‰ for deuterium.

  19. Forensic Stable Isotope Biogeochemistry

    Science.gov (United States)

    Cerling, Thure E.; Barnette, Janet E.; Bowen, Gabriel J.; Chesson, Lesley A.; Ehleringer, James R.; Remien, Christopher H.; Shea, Patrick; Tipple, Brett J.; West, Jason B.

    2016-06-01

    Stable isotopes are being used for forensic science studies, with applications to both natural and manufactured products. In this review we discuss how scientific evidence can be used in the legal context and where the scientific progress of hypothesis revisions can be in tension with the legal expectations of widely used methods for measurements. Although this review is written in the context of US law, many of the considerations of scientific reproducibility and acceptance of relevant scientific data span other legal systems that might apply different legal principles and therefore reach different conclusions. Stable isotopes are used in legal situations for comparing samples for authenticity or evidentiary considerations, in understanding trade patterns of illegal materials, and in understanding the origins of unknown decedents. Isotope evidence is particularly useful when considered in the broad framework of physiochemical processes and in recognizing regional to global patterns found in many materials, including foods and food products, drugs, and humans. Stable isotopes considered in the larger spatial context add an important dimension to forensic science.

  20. Prevalence of Besnoitia besnoiti antibodies in bovine sera and milk ...

    African Journals Online (AJOL)

    ADEYEYE

    2014-02-04

    Feb 4, 2014 ... to pass undiagnosed, antibody detection is required for screening the ... livestock in the country. Materials and .... majority of host animals (Cantu-Martinez et al., ... biologically stable IgA and IgM could be transferred to infants ...

  1. A 20 year independent record of sea surface temperature for climate from Along-Track Scanning Radiometers

    Science.gov (United States)

    Merchant, Christopher J.; Embury, Owen; Rayner, Nick A.; Berry, David I.; Corlett, Gary K.; Lean, Katie; Veal, Karen L.; Kent, Elizabeth C.; Llewellyn-Jones, David T.; Remedios, John J.; Saunders, Roger

    2012-12-01

    A new record of sea surface temperature (SST) for climate applications is described. This record provides independent corroboration of global variations estimated from SST measurements made in situ. Infrared imagery from Along-Track Scanning Radiometers (ATSRs) is used to create a 20 year time series of SST at 0.1° latitude-longitude resolution, in the ATSR Reprocessing for Climate (ARC) project. A very high degree of independence of in situ measurements is achieved via physics-based techniques. Skin SST and SST estimated for 20 cm depth are provided, with grid cell uncertainty estimates. Comparison with in situ data sets establishes that ARC SSTs generally have bias of order 0.1 K or smaller. The precision of the ARC SSTs is 0.14 K during 2003 to 2009, from three-way error analysis. Over the period 1994 to 2010, ARC SSTs are stable, with better than 95% confidence, to within 0.005 K yr-1(demonstrated for tropical regions). The data set appears useful for cleanly quantifying interannual variability in SST and major SST anomalies. The ARC SST global anomaly time series is compared to the in situ-based Hadley Centre SST data set version 3 (HadSST3). Within known uncertainties in bias adjustments applied to in situ measurements, the independent ARC record and HadSST3 present the same variations in global marine temperature since 1996. Since the in situ observing system evolved significantly in its mix of measurement platforms and techniques over this period, ARC SSTs provide an important corroboration that HadSST3 accurately represents recent variability and change in this essential climate variable.

  2. Spatiotemporal Variability of Earth's Radiation Balance Components from Russian Radiometer IKOR-M

    Science.gov (United States)

    Cherviakov, M.

    2016-12-01

    The radiometer IKOR-M was created in National Research Saratov State University for satellite monitoring of the outgoing reflected short-wave radiation, which is one of the components of Earth's radiation budget. Such information can be used in different models of long-term weather forecasts, in researches of climate change trends and in calculation of absorbed solar radiation values and albedo of the Earth-atmosphere system. The IKOR-M product archive is available online at all times. A searchable catalogue of data products is continually updated and users may search and download data products via the Earth radiation balance components research laboratory website as soon as they become available. Two series of measurements from two different IKOR-M are available. The first radiometer had worked from October 2009 to August 2014 and second - from August 2014 to the present. Therefore, there is a period when both radiometers work at the same time. Top-of-atmosphere fluxes deduced from the "Meteor-M" No 1 measurement in August, 2014 show very good agreement with the fluxes determined from "Meteor-M" No 2. The scale relationship of the IKOR-M radiometers on "Meteor - M" No 1 and No 2 satellites found by comparing of the global distribution maps for monthly averaged albedo values. The seasonal and interannual variations of OSR, albedo and ASR were discussed. The variations between SW radiation budget components seem to be within observational uncertainty and natural variability governed by cloudiness, water vapor and aerosol variations. It was assessed spatial and temporal variations of albedo and the absorbed solar radiation over different regions. Latitudinal distributions of albedo and ASR were estimated in more detail. Meridional cross sections over oceans and land were used separately for this estimation. It was shown that the albedo and ASR data received from the radiometer IKOR-M can be used to detect El Nino in the Pacific Ocean. The reported study was funded by

  3. Radiolabelled antibody imaging

    International Nuclear Information System (INIS)

    Perkins, A.C.

    1986-01-01

    A steadily growing number of tumor-associated antigens are used to raise antibodies used for the detection of human tumors by external imaging, a technique termed immunoscintigraphy. The majority of these clinical antibody studies are performed using Iodine-131, which is cheap, readily available and easily attached to protein. It has the disadvantage of having a high energy gamma emission (365 keV) which is poorly detected by modern cameras, so that increasing use is now being made of more appropriate labels with lower energies for imaging, such as Iodine-123, Indium-111 and Technetium-99m. A number of research centres in the United Kingdom are currently involved in the production of tumor-associated monoclonal antibodies, only a small number of which are finally selected for diagnostic use. These developments represent a major area of advancement in Nuclear Medicine and when used for imaging are capable of providing diagnostic information complimentary to other diagnostic techniques

  4. Technetium-99m pertechnetate - a tracer for radiolabelling antibody for inflammation detection

    International Nuclear Information System (INIS)

    Shaharuddin Mohd; Wan Hamirul Bahrin Wan Kamal; Shahrin A Hamid; Ang Woan Tze; Rosnani Hashim

    1999-01-01

    The polyclonal antibody, Human Immunoglobulin G (HlgG) was reduced by using 2-mercaptoethanol with molar ratio of 1000:1 (i.e. mercaptoethanol:antibody). The reduction of the antibody, was carried out for 30 minutes at room temperature. The reduced antibody was purified by using Sephadex G-25 fine column. The antibody kit for the detection of inflammation was prepared aseptically in Class 1 Laminar Flow cabinet. The kit passed the sterility test. Upon reconstitution of the antibody kit with sodium pertechnetate-99m ( 99m Tc) solution, the labelling efficiency obtained was more than 95%. This preparation was stable up to 24-hour stored at room temperature. Gamma camera scans showed the accumulation of technetium-99m labelled antibody ( 99m Tc-HIgG) at the turpentine-induced inflammation of female Sprague-Dawley rats. This indicated the possibility of using 99m Tc-HIgG for inflammation detection. (author)

  5. Antibody informatics for drug discovery

    DEFF Research Database (Denmark)

    Shirai, Hiroki; Prades, Catherine; Vita, Randi

    2014-01-01

    to the antibody science in every project in antibody drug discovery. Recent experimental technologies allow for the rapid generation of large-scale data on antibody sequences, affinity, potency, structures, and biological functions; this should accelerate drug discovery research. Therefore, a robust bioinformatic...... infrastructure for these large data sets has become necessary. In this article, we first identify and discuss the typical obstacles faced during the antibody drug discovery process. We then summarize the current status of three sub-fields of antibody informatics as follows: (i) recent progress in technologies...... for antibody rational design using computational approaches to affinity and stability improvement, as well as ab-initio and homology-based antibody modeling; (ii) resources for antibody sequences, structures, and immune epitopes and open drug discovery resources for development of antibody drugs; and (iii...

  6. Antithyroglobulin Antibodies and Antimicrosomal Antibodies in Various Thyroid Diseases

    International Nuclear Information System (INIS)

    Lee, Gwon Jun; Hong, Key Sak; Choi, Kang Won; Lee, Kyu; Koh, Chang Soon; Lee, Mun Ho; Park, Sung Hoe; Chi, Je Geun; Lee, Sang Kook

    1979-01-01

    The authors investigated the incidence of antithyroglobulin antibodies and antibodies and antimicrosomal antibodies measured by tanned red cell hemagglutination method in subjects suffering from various thyroid disorders. 1) In 15 normal patients, neither suffering from any thyroid diseases nor from any other autoimmune disorders, the antithyroglobulin antibodies were all negative, but the antimicrosomal antibody was positive only in one patient (6.7%). 2) The antithyroglobulin antibodies were positive in 31.5% (34 patients) of 108 patients with various thyroid diseases, and the antimicrosomal antibodies were positive in 37.0% (40 patients). 3) of the 25 patients with Graves' diseases, 7 patients (28.0%) showed positive for the antithyroglobulin antibodies, and 9 (36.0%) for the antimicrosomal antibodies. There was no definite differences in clinical and thyroid functions between the groups with positive and negative results. 4) Both antibodies were positive in 16 (88.9%) and 17 (94.4%) patients respectively among 18 patients with Hashimoto's thyroiditis, all of them were diagnosed histologically. 5) Three out of 33 patients with thyroid adenoma showed positive antibodies, and 3 of 16 patients with thyroid carcinoma revealed positive antibodies. 6) TRCH antibodies demonstrated negative results in 2 patients with subacute thyroiditis, but positive in one patient with idiopathic primary myxedema. 7) The number of patients with high titers(>l:802) was 16 for antithyroglobulin antibody, and 62.5% (10 patients) of which was Hashimoto's thyroiditis. Thirteen (65.0) of 20 patients with high titers (>l:802) for antimicrosomal antibody was Hashimoto's thyroiditis. TRCH test is a simple, sensitive method, and has high reliability and reproducibility. The incidences and titers of antithyroglobulin antibody and antimicrosomal antibody are especially high in Hashimoto's thyroiditis.

  7. Antithyroglobulin Antibodies and Antimicrosomal Antibodies in Various Thyroid Diseases

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Gwon Jun; Hong, Key Sak; Choi, Kang Won; Lee, Kyu; Koh, Chang Soon; Lee, Mun Ho; Park, Sung Hoe; Chi, Je Geun; Lee, Sang Kook [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    1979-03-15

    The authors investigated the incidence of antithyroglobulin antibodies and antibodies and antimicrosomal antibodies measured by tanned red cell hemagglutination method in subjects suffering from various thyroid disorders. 1) In 15 normal patients, neither suffering from any thyroid diseases nor from any other autoimmune disorders, the antithyroglobulin antibodies were all negative, but the antimicrosomal antibody was positive only in one patient (6.7%). 2) The antithyroglobulin antibodies were positive in 31.5% (34 patients) of 108 patients with various thyroid diseases, and the antimicrosomal antibodies were positive in 37.0% (40 patients). 3) of the 25 patients with Graves' diseases, 7 patients (28.0%) showed positive for the antithyroglobulin antibodies, and 9 (36.0%) for the antimicrosomal antibodies. There was no definite differences in clinical and thyroid functions between the groups with positive and negative results. 4) Both antibodies were positive in 16 (88.9%) and 17 (94.4%) patients respectively among 18 patients with Hashimoto's thyroiditis, all of them were diagnosed histologically. 5) Three out of 33 patients with thyroid adenoma showed positive antibodies, and 3 of 16 patients with thyroid carcinoma revealed positive antibodies. 6) TRCH antibodies demonstrated negative results in 2 patients with subacute thyroiditis, but positive in one patient with idiopathic primary myxedema. 7) The number of patients with high titers(>l:802) was 16 for antithyroglobulin antibody, and 62.5% (10 patients) of which was Hashimoto's thyroiditis. Thirteen (65.0) of 20 patients with high titers (>l:802) for antimicrosomal antibody was Hashimoto's thyroiditis. TRCH test is a simple, sensitive method, and has high reliability and reproducibility. The incidences and titers of antithyroglobulin antibody and antimicrosomal antibody are especially high in Hashimoto's thyroiditis.

  8. Prediction of Antibody Epitopes

    DEFF Research Database (Denmark)

    Nielsen, Morten; Marcatili, Paolo

    2015-01-01

    Antibodies recognize their cognate antigens in a precise and effective way. In order to do so, they target regions of the antigenic molecules that have specific features such as large exposed areas, presence of charged or polar atoms, specific secondary structure elements, and lack of similarity...... to self-proteins. Given the sequence or the structure of a protein of interest, several methods exploit such features to predict the residues that are more likely to be recognized by an immunoglobulin.Here, we present two methods (BepiPred and DiscoTope) to predict linear and discontinuous antibody...

  9. Antibody affinity maturation

    DEFF Research Database (Denmark)

    Skjødt, Mette Louise

    Yeast surface display is an effective tool for antibody affinity maturation because yeast can be used as an all-in-one workhorse to assemble, display and screen diversified antibody libraries. By employing the natural ability of yeast Saccharomyces cerevisiae to efficiently recombine multiple DNA...... laboratory conditions. A particular emphasis was put on using molecular techniques in conjunction with microenvironmental measurements (O2, pH, irradiance), a combination that is rarely found but provides a much more detailed understanding of “cause and effect” in complex natural systems...

  10. Towards a long-term Science Exploitation Plan for the Sea and Land Surface Temperature Radiometer on Sentinel-3 and the Along-Track Scanning Radiometers

    Science.gov (United States)

    Remedios, John J.; Llewellyn-Jones, David

    2014-05-01

    The Sea and Land Surface Temperature Radiometer (SLSTR) on Sentinel-3 is the latest satellite instrument in a series of dual-angle optical and thermal sensors, the Along-Track Scanning Radiometers (ATSRs). Operating on Sentinel-3, the SLSTR has a number of significant improvements compared to the original ATSRs including wider swaths for nadir and dual angles, emphasis on all surface temperature domains, dedicated fire channels and additional cloud channels. The SLSTR therefore provides some excellent opportunities to extend science undertaken with the ATSRs whilst also providing long-term data sets to investigate climate change. The European Space Agency, together with the Department of Energy and Climate Change, sponsored the production of an Exploitation Plan for the ATSRs. In the last year, this been extended to cover the SLSTR also. The plan enables UK and European member states to plan activities related to SLSTR in a long-term context. Covering climate change, oceanography, land surface, atmosphere and cryosphere science, particular attention is paid to the exploitation of long-term data sets. In the case of SLSTR, relevant products include sea, land, lake and ice surface temperatures; aerosols and clouds; fires and gas flares; land surface reflectances. In this presentation, the SLSTR and ATSR science Exploitation Plan will be outlined with emphasis on SLSTR science opportunities, on appropriate co-ordinating mechanisms and on example implementation plans. Particular attention will be paid to the challenges of linking ATSR records with SLSTR to provide consistent long-term data sets, and on the international context of such data sets. The exploitation plan approach to science may prove relevant and useful for other Sentinel instruments.

  11. Compositions, antibodies, asthma diagnosis methods, and methods for preparing antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Jin, Hongjun; Zangar, Richard C.

    2017-01-17

    Methods for preparing an antibody are provided with the method including incorporating 3-bromo-4-hydroxy-benzoic acid into a protein to form an antigen, immunizing a mammalian host with the antigen, and recovering an antibody having an affinity for the antigen from the host. Antibodies having a binding affinity for a monohalotyrosine are provided as well as composition comprising an antibody bound with monohalotyrosine. Compositions comprising a protein having a 3-bromo-4-hydroxy-benzoic acid moiety are also provided. Methods for evaluating the severity of asthma are provide with the methods including analyzing sputum of a patient using an antibody having a binding affinity for monohalotyrosine, and measuring the amount of antibody bound to protein. Methods for determining eosinophil activity in bodily fluid are also provided with the methods including exposing bodily fluid to an antibody having a binding affinity for monohalotyrosine, and measuring the amount of bound antibody to determine the eosinophil activity.

  12. LC-MS/MS strategies for therapeutic antibodies and investigation into the quantitative impact of antidrug-antibodies.

    Science.gov (United States)

    Ewles, Matthew; Mannu, Ranbir; Fox, Chris; Stanta, Johannes; Evans, Graeme; Goodwin, Lee; Duffy, James; Bell, Len; Estdale, Sian; Firth, David

    2016-12-01

    We aimed to establish novel, high-throughput LC-MS/MS strategies for quantification of monoclonal antibodies in human serum and examine the potential impact of antidrug antibodies. We present two strategies using a thermally stable immobilized trypsin. The first strategy uses whole serum digestion and the second introduces Protein G enrichment to improve the selectivity. The impact of anti-trastuzumab antibodies on the methods was tested. Whole serum digestion has been validated for trastuzumab (LLOQ 0.25 µg/ml). Protein G enrichment has been validated for trastuzumab (LLOQ 0.1 µg/ml), bevacizumab (LLOQ 0.1 µg/ml) and adalimumab (LLOQ 0.25 µg/ml). We have shown the potential for anti-drug antibodies to impact on the quantification and we have subsequently established a strategy to overcome this impact where total quantification is desired.

  13. The effects of tether placement on antibody stability on surfaces

    Science.gov (United States)

    Grawe, Rebecca W.; Knotts, Thomas A.

    2017-06-01

    Despite their potential benefits, antibody microarrays have fallen short of performing reliably and have not found widespread use outside of the research setting. Experimental techniques have been unable to determine what is occurring on the surface of an atomic level, so molecular simulation has emerged as the primary method of investigating protein/surface interactions. Simulations of small proteins have indicated that the stability of the protein is a function of the residue on the protein where a tether is placed. The purpose of this research is to see whether these findings also apply to antibodies, with their greater size and complexity. To determine this, 24 tethering locations were selected on the antibody Protein Data Bank (PDB) ID: 1IGT. Replica exchange simulations were run on two different surfaces, one hydrophobic and one hydrophilic, to determine the degree to which these tethering sites stabilize or destabilize the antibody. Results showed that antibodies tethered to hydrophobic surfaces were in general less stable than antibodies tethered to hydrophilic surfaces. Moreover, the stability of the antibody was a function of the tether location on hydrophobic surfaces but not hydrophilic surfaces.

  14. Prediction of antibody persistency from antibody titres to natalizumab

    DEFF Research Database (Denmark)

    Jensen, Poul Erik H; Koch-Henriksen, Nils; Sellebjerg, Finn

    2012-01-01

    In a subgroup of patients with multiple sclerosis natalizumab therapy causes generation of anti-natalizumab antibodies that may be transient or persistent. It is recommended to discontinue natalizumab therapy in persistently antibody-positive patients.......In a subgroup of patients with multiple sclerosis natalizumab therapy causes generation of anti-natalizumab antibodies that may be transient or persistent. It is recommended to discontinue natalizumab therapy in persistently antibody-positive patients....

  15. Human monoclonal antibodies: the residual challenge of antibody immunogenicity.

    Science.gov (United States)

    Waldmann, Herman

    2014-01-01

    One of the major reasons for seeking human monoclonal antibodies has been to eliminate immunogenicity seen with rodent antibodies. Thus far, there has yet been no approach which absolutely abolishes that risk for cell-binding antibodies. In this short article, I draw attention to classical work which shows that monomeric immunoglobulins are intrinsically tolerogenic if they can be prevented from creating aggregates or immune complexes. Based on these classical studies two approaches for active tolerization to therapeutic antibodies are described.

  16. Applying Advances in GPM Radiometer Intercalibration and Algorithm Development to a Long-Term TRMM/GPM Global Precipitation Dataset

    Science.gov (United States)

    Berg, W. K.

    2016-12-01

    The Global Precipitation Mission (GPM) Core Observatory, which was launched in February of 2014, provides a number of advances for satellite monitoring of precipitation including a dual-frequency radar, high frequency channels on the GPM Microwave Imager (GMI), and coverage over middle and high latitudes. The GPM concept, however, is about producing unified precipitation retrievals from a constellation of microwave radiometers to provide approximately 3-hourly global sampling. This involves intercalibration of the input brightness temperatures from the constellation radiometers, development of an apriori precipitation database using observations from the state-of-the-art GPM radiometer and radars, and accounting for sensor differences in the retrieval algorithm in a physically-consistent way. Efforts by the GPM inter-satellite calibration working group, or XCAL team, and the radiometer algorithm team to create unified precipitation retrievals from the GPM radiometer constellation were fully implemented into the current version 4 GPM precipitation products. These include precipitation estimates from a total of seven conical-scanning and six cross-track scanning radiometers as well as high spatial and temporal resolution global level 3 gridded products. Work is now underway to extend this unified constellation-based approach to the combined TRMM/GPM data record starting in late 1997. The goal is to create a long-term global precipitation dataset employing these state-of-the-art calibration and retrieval algorithm approaches. This new long-term global precipitation dataset will incorporate the physics provided by the combined GPM GMI and DPR sensors into the apriori database, extend prior TRMM constellation observations to high latitudes, and expand the available TRMM precipitation data to the full constellation of available conical and cross-track scanning radiometers. This combined TRMM/GPM precipitation data record will thus provide a high-quality high

  17. ANA (Antinuclear Antibody Test)

    Science.gov (United States)

    ... as ratios. For example, the result 1:320 means that one part blood sample was mixed with 320 parts of a diluting ... name "antinuclear". My doctor told me my ANA test is ... normal concentration of these antibodies. This is one of the tools in diagnosing lupus as well ...

  18. Monoclonal antibodies in myeloma

    DEFF Research Database (Denmark)

    Sondergeld, P.; van de Donk, N. W. C. J.; Richardson, P. G.

    2015-01-01

    The development of monoclonal antibodies (mAbs) for the treatment of disease goes back to the vision of Paul Ehrlich in the late 19th century; however, the first successful treatment with a mAb was not until 1982, in a lymphoma patient. In multiple myeloma, mAbs are a very recent and exciting add...

  19. Antibodies Targeting EMT

    Science.gov (United States)

    2017-10-01

    these unusual antibodies can effectively be displayed on the cell surface. 5 Additionally, we successfully prepared cDNA from lymphocytes derived...from cow peripheral blood, spleen, and lymph nodes, amplified this cDNA by PCR with VH gene specific primers, and this “library” has been cloned into

  20. Antibody Blood Tests

    Science.gov (United States)

    ... out for sure? If antibody tests and/or symptoms suggest celiac disease, the physician needs to establish the diagnosis by ... who is still experiencing symptoms, to establish the diagnosis or to rule out celiac disease as a part of establishing another diagnosis. Find ...

  1. Antinuclear Antibodies (ANA)

    Science.gov (United States)

    ... MACRA MACRAlerts MACRA FAQs MACRA Glossary MACRA Resources Position Statements Insurance Advocacy Current Issues Tools & Resources Practice Resources ... a medical or health condition. Resources Antinuclear Antibodies (ANA) in Spanish (Español) Download Print-Friendly PDF ... Join Donate © 2018 American College ...

  2. Next Generation Antibody Therapeutics Using Bispecific Antibody Technology.

    Science.gov (United States)

    Igawa, Tomoyuki

    2017-01-01

    Nearly fifty monoclonal antibodies have been approved to date, and the market for monoclonal antibodies is expected to continue to grow. Since global competition in the field of antibody therapeutics is intense, we need to establish novel antibody engineering technologies to provide true benefit for patients, with differentiated product values. Bispecific antibodies are among the next generation of antibody therapeutics that can bind to two different target antigens by the two arms of immunoglobulin G (IgG) molecule, and are thus believed to be applicable to various therapeutic needs. Until recently, large scale manufacturing of human IgG bispecific antibody was impossible. We have established a technology, named asymmetric re-engineering technology (ART)-Ig, to enable large scale manufacturing of bispecific antibodies. Three examples of next generation antibody therapeutics using ART-Ig technology are described. Recent updates on bispecific antibodies against factor IXa and factor X for the treatment of hemophilia A, bispecific antibodies against a tumor specific antigen and T cell surface marker CD3 for cancer immunotherapy, and bispecific antibodies against two different epitopes of soluble antigen with pH-dependent binding property for the elimination of soluble antigen from plasma are also described.

  3. Anti-smooth muscle antibody

    Science.gov (United States)

    ... gov/ency/article/003531.htm Anti-smooth muscle antibody To use the sharing features on this page, please enable JavaScript. Anti-smooth muscle antibody is a blood test that detects the presence ...

  4. Tabhu: tools for antibody humanization.

    KAUST Repository

    Olimpieri, Pier Paolo; Marcatili, Paolo; Tramontano, Anna

    2014-01-01

    for antibody humanization. Tabhu includes tools for human template selection, grafting, back-mutation evaluation, antibody modelling and structural analysis, helping the user in all the critical steps of the humanization experiment protocol. AVAILABILITY: http

  5. Optimization of antibody immobilization for on-line or off-line immunoaffinity chromatography

    DEFF Research Database (Denmark)

    Beyer, Natascha Helena; Schou, Christian; Højrup, Peter

    2009-01-01

    -POROS. Protein G-based matrices are very stable showing essentially no decline in performance after 50 application-wash-elution-reequilibration cycles and being easily prepared within 2-3 h of working time with a typical antibody coupling yield of above 80%. In off-line applications where constant flow....... A systematic study was conducted to determine the most versatile antibody immobilization method for use in on-line and off-line IA chromatography applications using commonly accessible immobilization methods. Four chemistries were examined using polyclonal and monoclonal antibodies and antibody fragments. We...

  6. Theory of stable allocations

    Directory of Open Access Journals (Sweden)

    Pantelić Svetlana

    2014-01-01

    Full Text Available The Swedish Royal Academy awarded the 2012 Nobel Prize in Economics to Lloyd Shapley and Alvin Roth, for the theory of stable allocations and the practice of market design. These two American researchers worked independently from each other, combining basic theory and empirical investigations. Through their experiments and practical design they generated a flourishing field of research and improved the performance of many markets. Born in 1923 in Cambridge, Massachusetts, Shapley defended his doctoral thesis at Princeton University in 1953. For many years he worked at RAND, and for more than thirty years he was a professor at UCLA University. He published numerous scientific papers, either by himself or in cooperation with other economists.

  7. High spatial resolution upgrade of the electron cyclotron emission radiometer for the DIII-D tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Truong, D. D., E-mail: dtruong@wisc.edu [Department of Engineering Physics, University of Wisconsin-Madison, Madison, Wisconsin 53706 (United States); Austin, M. E. [Institute for Fusion Studies, University of Texas, Austin, Texas, 78712 (United States)

    2014-11-15

    The 40-channel DIII-D electron cyclotron emission (ECE) radiometer provides measurements of T{sub e}(r,t) at the tokamak midplane from optically thick, second harmonic X-mode emission over a frequency range of 83–130 GHz. The frequency spacing of the radiometer's channels results in a spatial resolution of ∼1–3 cm, depending on local magnetic field and electron temperature. A new high resolution subsystem has been added to the DIII-D ECE radiometer to make sub-centimeter (0.6–0.8 cm) resolution T{sub e} measurements. The high resolution subsystem branches off from the regular channels’ IF bands and consists of a microwave switch to toggle between IF bands, a switched filter bank for frequency selectivity, an adjustable local oscillator and mixer for further frequency down-conversion, and a set of eight microwave filters in the 2–4 GHz range. Higher spatial resolution is achieved through the use of a narrower (200 MHz) filter bandwidth and closer spacing between the filters’ center frequencies (250 MHz). This configuration allows for full coverage of the 83–130 GHz frequency range in 2 GHz bands. Depending on the local magnetic field, this translates into a “zoomed-in” analysis of a ∼2–4 cm radial region. Expected uses of these channels include mapping the spatial dependence of Alfven eigenmodes, geodesic acoustic modes, and externally applied magnetic perturbations. Initial T{sub e} measurements, which demonstrate that the desired resolution is achieved, are presented.

  8. Total column water vapor estimation over land using radiometer data from SAC-D/Aquarius

    Science.gov (United States)

    Epeloa, Javier; Meza, Amalia

    2018-02-01

    The aim of this study is retrieving atmospheric total column water vapor (CWV) over land surfaces using a microwave radiometer (MWR) onboard the Scientific Argentine Satellite (SAC-D/Aquarius). To research this goal, a statistical algorithm is used for the purpose of filtering the study region according to the climate type. A log-linear relationship between the brightness temperatures of the MWR and CWV obtained from Global Navigation Satellite System (GNSS) measurements was used. In this statistical algorithm, the retrieved CWV is derived from the Argentinian radiometer's brightness temperature which works at 23.8 GHz and 36.5 GHz, and taking into account CWVs observed from GNSS stations belonging to a region sharing the same climate type. We support this idea, having found a systematic effect when applying the algorithm; it was generated for one region using the previously mentioned criteria, however, it should be applied to additional regions, especially those with other climate types. The region we analyzed is in the Southeastern United States of America, where the climate type is Cfa (Köppen - Geiger classification); this climate type includes moist subtropical mid-latitude climates, with hot, muggy summers and frequent thunderstorms. However, MWR only contains measurements taken from over ocean surfaces; therefore the determination of water vapor over land is an important contribution to extend the use of the SAC-D/Aquarius radiometer measurements beyond the ocean surface. The CWVs computed by our algorithm are compared against radiosonde CWV observations and show a bias of about -0.6 mm, a root mean square (rms) of about 6 mm and a correlation of 0.89.

  9. Maritime Aerosol optical properties measured by ship-borne sky radiometer

    Science.gov (United States)

    Aoki, K.

    2017-12-01

    Maritime aerosols play an important role in the earth climate change. We started the measurements of aerosol optical properties since 1994 by using ship-borne sky radiometer (POM-01 MK-II and III; Prede Co. Ltd., Japan) over the ocean. We report the results of an aerosol optical properties over the ocean by using Research Vessel of the ship-borne sky radiometers. Aerosol optical properties observation were made in MR10-02 to MR16-09 onboard the R/V Mirai, JAMSTEC. The sky radiometer measure the direct and diffuse solar radiance with seven interference filters (0.315, 0.4, 0.5, 0.675, 0.87, 0.94, and 1.02 µm). Observation interval was made every five minutes by once, only in daytime under the clear sky conditions. GPS provides the position with longitude and latitude and heading direction of the vessel, and azimuth and elevation angle of the sun. The aerosol optical properties were computed using the SKYRAD.pack version 4.2. The obtained Aerosol optical properties (Aerosol optical thickness, Ångström exponent, Single scattering albedo, and etc.) and size distribution volume clearly showed spatial and temporal variability over the ocean. Aerosol optical thickness found over the near the coast (Asia and Tropical area) was high and variable. The size distribution volume have peaks at small particles at Asian coast and large particles at Tropical coast area. We provide the information, in this presentation, on the aerosol optical properties measurements with temporal and spatial variability in the Maritime Aerosol. This project is validation satellite of GCOM-C/SGLI, JAXA and other. The GCOM-C satellite scheduled to be launched in 2017 JFY.

  10. Intercomparison of stratospheric temperature profiles from a ground-based microwave radiometer with other techniques

    Directory of Open Access Journals (Sweden)

    F. Navas-Guzmán

    2017-11-01

    Full Text Available In this work the stratospheric performance of a relatively new microwave temperature radiometer (TEMPERA has been evaluated. With this goal in mind, almost 3 years of temperature measurements (January 2014–September 2016 from the TEMPERA radiometer were intercompared with simultaneous measurements from other techniques: radiosondes, MLS satellite and Rayleigh lidar. This intercomparison campaign was carried out at the aerological station of MeteoSwiss at Payerne (Switzerland. In addition, the temperature profiles from TEMPERA were used to validate the temperature outputs from the SD-WACCM model. The results showed in general a very good agreement between TEMPERA and the different instruments and the model, with a high correlation (higher than 0.9 in the temperature evolution at different altitudes between TEMPERA and the different data sets. An annual pattern was observed in the stratospheric temperature with generally higher temperatures in summer than in winter and with a higher variability during winter. A clear change in the tendency of the temperature deviations was detected in summer 2015, which was due to the repair of an attenuator in the TEMPERA spectrometer. The mean and the standard deviations of the temperature differences between TEMPERA and the different measurements were calculated for two periods (before and after the repair in order to quantify the accuracy and precision of this radiometer over the campaign period. The results showed absolute biases and standard deviations lower than 2 K for most of the altitudes. In addition, comparisons proved the good performance of TEMPERA in measuring the temperature in the stratosphere.

  11. Antibodies from plants for bionanomaterials

    OpenAIRE

    Edgue, G.; Twyman, R.M.; Beiss, V.; Fischer, R.; Sack, M.

    2017-01-01

    Antibodies are produced as part of the vertebrate adaptive immune response and are not naturally made by plants. However, antibody DNA sequences can be introduced into plants, and together with laboratory technologies that allow the design of antibodies recognizing any conceivable molecular structure, plants can be used as green factories' to produce any antibody at all. The advent of plant-based transient expression systems in particular allows the rapid, convenient, and safe production of a...

  12. Bi-stable optical actuator

    Science.gov (United States)

    Holdener, Fred R.; Boyd, Robert D.

    2000-01-01

    The present invention is a bi-stable optical actuator device that is depowered in both stable positions. A bearing is used to transfer motion and smoothly transition from one state to another. The optical actuator device may be maintained in a stable position either by gravity or a restraining device.

  13. Usefulness of the infrared heterodyne radiometer in remote sensing of atmospheric pollutants.

    Science.gov (United States)

    Menzies, R. T.; Shumate, M. S.

    1971-01-01

    The application of narrow-band optical receivers to the problem of sensing atmospheric pollution is discussed. The emission/absorption lines of many major atmospheric pollutant molecules overlap the operating frequency bands of CO2 laser and CO laser heterodyne receivers. Several remote pollution sensing systems which are based upon utilization of these spectral overlaps are described, and an analysis of their potential is presented. The possibility of using other lasers (e.g.: the PbSnTe tunable diode laser) as local oscillators is also considered. Results of laboratory experiments with a CO2 laser heterodyne radiometer are presented.

  14. Global silicate mineralogy of the Moon from the Diviner lunar radiometer.

    Science.gov (United States)

    Greenhagen, Benjamin T; Lucey, Paul G; Wyatt, Michael B; Glotch, Timothy D; Allen, Carlton C; Arnold, Jessica A; Bandfield, Joshua L; Bowles, Neil E; Donaldson Hanna, Kerri L; Hayne, Paul O; Song, Eugenie; Thomas, Ian R; Paige, David A

    2010-09-17

    We obtained direct global measurements of the lunar surface using multispectral thermal emission mapping with the Lunar Reconnaissance Orbiter Diviner Lunar Radiometer Experiment. Most lunar terrains have spectral signatures that are consistent with known lunar anorthosite and basalt compositions. However, the data have also revealed the presence of highly evolved, silica-rich lunar soils in kilometer-scale and larger exposures, expanded the compositional range of the anorthosites that dominate the lunar crust, and shown that pristine lunar mantle is not exposed at the lunar surface at the kilometer scale. Together, these observations provide compelling evidence that the Moon is a complex body that has experienced a diverse set of igneous processes.

  15. New Approach for Monitoring Seismic and Volcanic Activities Using Microwave Radiometer Data

    Science.gov (United States)

    Maeda, Takashi; Takano, Tadashi

    Interferograms formed from the data of satellite-borne synthetic aperture radar (SAR) enable us to detect slight land-surface deformations related to volcanic eruptions and earthquakes. Currently, however, we cannot determine when land-surface deformations occurred with high time resolution since the time lag between two scenes of SAR used to form interferograms is longer than the recurrent period of the satellite carrying it (several tens of days). In order to solve this problem, we are investigating new approach to monitor seismic and vol-canic activities with higher time resolution from satellite-borne sensor data, and now focusing on a satellite-borne microwave radiometer. It is less subject to clouds and rainfalls over the ground than an infrared spectrometer, so more suitable to observe an emission from land sur-faces. With this advantage, we can expect that thermal microwave energy by increasing land surface temperatures is detected before a volcanic eruption. Additionally, laboratory experi-ments recently confirmed that rocks emit microwave energy when fractured. This microwave energy may result from micro discharges in the destruction of materials, or fragment motions with charged surfaces of materials. We first extrapolated the microwave signal power gener-ated by rock failures in an earthquake from the experimental results and concluded that the microwave signals generated by rock failures near the land surface are strong enough to be detected by a satellite-borne radiometer. Accordingly, microwave energy generated by rock failures associated with a seismic activity is likely to be detected as well. However, a satellite-borne microwave radiometer has a serious problem that its spatial res-olution is too coarse compared to SAR or an infrared spectrometer. In order to raise the possibility of detection, a new methodology to compensate the coarse spatial resolution is es-sential. Therefore, we investigated and developed an analysis method to detect local

  16. A Model for Estimation of Rain Rate on Tropical Land from TRMM Microwave Imager Radiometer Observations

    OpenAIRE

    C., PRABHAKARA; R., IACOVAZZI; J. M., YOO; K. M., KIM; NASA Goddard Space Flight Center; Center for Research on the Changing Earth System; EWHA Womans University; Science Systems and Applications, Inc.

    2005-01-01

    Over the tropical land regions scatter plots of the rain rate (R_), deduced from the TRMM Precipitation Radar (PR) versus the observed 85GHz brightness temperature (T_) made by the TRMM Microwave Imager (TMI) radiometer, for a period of a season over a given geographic region of 3°×5°(lat×lon), indicate that there are two maxima in rain rate. One strong maximum occurs when T_ has a value of about 220K, and the other weaker one when T_ is much colder ~150K. Also these two maxima are vividly re...

  17. Dual-Polarization, Multi-Frequency Antenna Array for use with Hurricane Imaging Radiometer

    Science.gov (United States)

    Little, John

    2013-01-01

    Advancements in common aperture antenna technology were employed to utilize its proprietary genetic algorithmbased modeling tools in an effort to develop, build, and test a dual-polarization array for Hurricane Imaging Radiometer (HIRAD) applications. Final program results demonstrate the ability to achieve a lightweight, thin, higher-gain aperture that covers the desired spectral band. NASA employs various passive microwave and millimeter-wave instruments, such as spectral radiometers, for a range of remote sensing applications, from measurements of the Earth's surface and atmosphere, to cosmic background emission. These instruments such as the HIRAD, SFMR (Stepped Frequency Microwave Radiometer), and LRR (Lightweight Rainfall Radiometer), provide unique data accumulation capabilities for observing sea surface wind, temperature, and rainfall, and significantly enhance the understanding and predictability of hurricane intensity. These microwave instruments require extremely efficient wideband or multiband antennas in order to conserve space on the airborne platform. In addition, the thickness and weight of the antenna arrays is of paramount importance in reducing platform drag, permitting greater time on station. Current sensors are often heavy, single- polarization, or limited in frequency coverage. The ideal wideband antenna will have reduced size, weight, and profile (a conformal construct) without sacrificing optimum performance. The technology applied to this new HIRAD array will allow NASA, NOAA, and other users to gather information related to hurricanes and other tropical storms more cost effectively without sacrificing sensor performance or the aircraft time on station. The results of the initial analysis and numerical design indicated strong potential for an antenna array that would satisfy all of the design requirements for a replacement HIRAD array. Multiple common aperture antenna methodologies were employed to achieve exceptional gain over the entire

  18. Intercomparison of characterization techniques of filter radiometers in the ultraviolet region

    International Nuclear Information System (INIS)

    Abu-Kassem, I.; Karha, P.; Harrison, N. J.; Nevas, S.; Hartree, W. S.

    2008-01-01

    Narrow-band filter radiometers at 248 nm, 313 nm, 330 nm and 368 nm wavelengths were used to compare calibration facilities of spectral (irradiance) responsivity at HUT, NPL and BNM-INM. The results are partly in agreement within the stated uncertainties. Use of demanding artefacts in the intercomparison revealed that the wavelength scales of the participating institutes deviate more than expected. Such effects cannot be seen in typical intercomparisons of spectral responsivity or spectral transmittance, where spectrally neutral samples are used.(author)

  19. An Optimal Beamforming Algorithm for Phased-Array Antennas Used in Multi-Beam Spaceborne Radiometers

    DEFF Research Database (Denmark)

    Iupikov, O. A.; Ivashina, M. V.; Pontoppidan, K.

    2015-01-01

    Strict requirements for future spaceborne ocean missions using multi-beam radiometers call for new antenna technologies, such as digital beamforming phased arrays. In this paper, we present an optimal beamforming algorithm for phased-array antenna systems designed to operate as focal plane arrays...... to a FPA feeding a torus reflector antenna (designed under the contract with the European Space Agency) and tested for multiple beams. The results demonstrate an improved performance in terms of the optimized beam characteristics, yielding much higher spatial and radiometric resolution as well as much...

  20. Measurement of radiosity coefficient by using an infrared radiometer and its application

    International Nuclear Information System (INIS)

    Okamoto, Yoshizo; Kaminaga, Fumito; Osakabe, Masahiro; Maekawa, Katsuhiro; Ishii, Toshimitsu; Ohoka, Norikazu; Eto, Motokuni.

    1989-01-01

    An infrared radiometer has been used for measuring and visualizing radiation temperature distribution of a surface in many fields as a remote sensing devices. Measured radiation flux is a summation of a emitted radiation and a reflection, which is called as a radiosity flux. The present paper shows characteristics of the radiosity of tested materials. And the infrared sensor is used to detect the small surface flaw and to measure the erosion rare of the graphite by ion beam injection and the temperature distribution of a cutter. (author)

  1. Measurement of radiosity coefficient by using an infrared radiometer and its application

    Energy Technology Data Exchange (ETDEWEB)

    Okamoto, Yoshizo; Kaminaga, Fumito; Osakabe, Masahiro; Maekawa, Katsuhiro; Ishii, Toshimitsu [Ibaraki Univ., Hitachi (Japan). Faculty of Engineering; Ohoka, Norikazu; Eto, Motokuni

    1989-12-01

    An infrared radiometer has been used for measuring and visualizing radiation temperature distribution of a surface in many fields as a remote sensing devices. Measured radiation flux is a summation of a emitted radiation and a reflection, which is called as a radiosity flux. The present paper shows characteristics of the radiosity of tested materials. And the infrared sensor is used to detect the small surface flaw and to measure the erosion rare of the graphite by ion beam injection and the temperature distribution of a cutter. (author).

  2. Sentinel-3 MWR Microwave Radiometer – Our contribution to the success of the Copernicus programme

    Directory of Open Access Journals (Sweden)

    M.A. Palacios

    2014-06-01

    Full Text Available The MWR builds, together with the SRAL altimeter, the S3 topography mission. The MWR, developed by EADS CASA Espacio as prime contractor, provides information for tropospheric path correction of SRAL measurements. MWR data can also be used for determining surface emissivity and soil moisture over land, surface energy budget investigations and ice characterization. The MWR instrument is a Noise Injection Radiometer (NIR, working at two frequencies (23.8/36.5 GHz, embarking a dual frequency horn antenna pointing to the cold sky for embedded autonomous calibration.

  3. The along track scanning radiometer for ERS-1 - Scan geometry and data simulation

    Science.gov (United States)

    Prata, A. J. Fred; Cechet, Robert P.; Barton, Ian J.; Llewellyn-Jones, David T.

    1990-01-01

    The first European remote-sensing satellite (ERS-1), due to be launched in 1990, will carry the along track scanning radiometer (ATSR), which has been specifically designed to give accurate satellite measurements of sea surface temperature (SST). Details of the novel scanning technique used by the ATSR are given, and data from the NOAA-9 AVHRR instrument are used to simulate raw ATSR imagery. Because of the high precision of the onboard blackbodies, the active cooling of the detectors, 12-b digitization, and dual-angle capability, the ATSR promises to achieve higher-accuracy satellite-derived SSTs than are currently available.

  4. Construction of a radiometer for pyroelectric detector and presentation of a model for detector design

    International Nuclear Information System (INIS)

    Siqueira, C.A. de.

    1987-01-01

    An expression has been developed for the pyroelectric voltage as a function of electric and thermal parameters of the detector. It has also been developed expressions for determination of unknown parameters from the experimentally obtained pyroelectric voltage curve as function of time and some other known information. It has also been shown figures of merit for characterization of the detectors, a study showing the detector performance dependence on each electric and thermal parameter and some illustrative experimental results. The radiometer designed and built for this work, is described. (author) [pt

  5. Synthetic peptides for antibody production

    NARCIS (Netherlands)

    Zegers, N.D.

    1995-01-01

    Synthetic peptides are useful tools for the generation of antibodies. The use of antibodies as specific reagents in inununochemical assays is widely applied. In this chapter, the application of synthetic peptides for the generation of antibodies is described. The different steps that lead to the

  6. Synthetic peptides for antibody production

    NARCIS (Netherlands)

    N.D. Zegers (Netty)

    1995-01-01

    textabstractSynthetic peptides are useful tools for the generation of antibodies. The use of antibodies as specific reagents in inununochemical assays is widely applied. In this chapter, the application of synthetic peptides for the generation of antibodies is described. The different steps

  7. Monoclonal antibodies to Pneumocystis carinii

    DEFF Research Database (Denmark)

    Kovacs, J A; Halpern, J L; Lundgren, B

    1989-01-01

    To increase understanding of the antigenic structure of Pneumocystis carinii, we developed monoclonal antibodies to rat and human P. carinii. The specificity of the antibodies was demonstrated by immunofluorescence and immunoblot studies. Only one of five monoclonal antibodies to rat P. carinii r...

  8. Forward Model Studies of Water Vapor Using Scanning Microwave Radiometers, Global Positioning System, and Radiosondes during the Cloudiness Intercomparison Experiment

    International Nuclear Information System (INIS)

    Mattioli, Vinia; Westwater, Ed R.; Gutman, S.; Morris, Victor R.

    2005-01-01

    Brightness temperatures computed from five absorption models and radiosonde observations were analyzed by comparing them with measurements from three microwave radiometers at 23.8 and 31.4 GHz. Data were obtained during the Cloudiness Inter-Comparison experiment at the U.S. Department of Energy's Atmospheric Radiation Measurement Program's (ARM) site in North-Central Oklahoma in 2003. The radiometers were calibrated using two procedures, the so-called instantaneous ?tipcal? method and an automatic self-calibration algorithm. Measurements from the radiometers were in agreement, with less than a 0.4-K difference during clear skies, when the instantaneous method was applied. Brightness temperatures from the radiometer and the radiosonde showed an agreement of less than 0.55 K when the most recent absorption models were considered. Precipitable water vapor (PWV) computed from the radiometers were also compared to the PWV derived from a Global Positioning System station that operates at the ARM site. The instruments agree to within 0.1 cm in PWV retrieval

  9. Multichannel heterodyne radiometers with fast-scanning backward-wave oscillators for ECE measurement on HT-7 tokamak

    International Nuclear Information System (INIS)

    Zhang, S.Y.; Poznyak, V.I.; Ploskirev, G.; Kalupin, D.; Wan, Y.X.; Xie, J.K.; Luo, J.R.; Li, J.G.; Gao, X.; Wan, B.N.; Zhang, X.D.; Wang, K.J.; Kuang, G.L.

    2001-01-01

    Two sets of fast-scanning heterodyne radiometer receiver systems employing backward-wave oscillators (BWOs) in 78-118 and 118-178 GHz were developed and installed for electron cyclotron emission (ECE) measurements on HT-7 superconducting tokamak. The double sideband (DSB) radiometer in 78-118 GHz measures 16 ECE frequency points with a scanning time period of 0.65 ms. The other radiometer in 118-178 GHz consists of one independent channel of DSB heterodyne receiver with intermediate frequency (IF) of 100-500 MHz and two channels of single sideband (SSB) heterodyne receiver that are sensitive to upper sideband and lower sideband individually; the IF frequency of the SSB channels are 1.5 GHz around the local oscillator frequencies with 1 GHz bandwidth. By employing a novel design, this unique radiometer measures 3 ECE frequency points at each of the 16 local oscillator frequency points in 118-178 GHz, and the full band can be swept in 0.65 ms period, thus the radiometer measures 48 ECE frequency points in 0.65 ms in principle. Each of the local oscillators' frequency points can be preset by program to meet specific physics interests. Horizontal view of ECE was installed to measure electron temperature profiles; vertically viewing optics along a perpendicular chord was also installed to study nonthermal ECE spectra. Preliminary measurement results were presented during ohmic and pellet injection plasmas

  10. Newly devised infrared radiometer (ERI type IR ground scanner) and the surface temperature of the Mihara crater, O-shima

    Energy Technology Data Exchange (ETDEWEB)

    Shimozuru, D [Earthquake Res. Inst., Univ. of Tokyo; Kagiyama, T

    1976-10-01

    The infrared radiometer, a remote sensing tool, can be successfully used to measure the surface temperature of a volcanic or geothermal area. Many of these devices are available commercially for industrial use but their application to volcano observations is limited due to a wide field of view which prohibits detailed examination of specific points. A commercial radiometer was mounted on a balloon theodolite with an electrically driven rotating base. A telescope was attached to the radiometer to permit monitoring of the field of view. Radiometer output can be recorded either on a magnetic tape data recorder or a strip chart recorder. The device is also useful for continuous monitoring of the temperature of a vent or fumarole. The observed temperatures are dependent upon the wave length of actual spatial temperature distribution, the field of view and the scanning speed. Detailed information of both a theoretical and an experimental nature is provided. The improved radiometer was utilized to observe surface temperature in the caldera of Miharayama, Oshima in March, 1976. It was found that the vent temperature was markedly lower than had previously been recorded, as was the average surface temperature.

  11. Potential of Future Hurricane Imaging Radiometer (HIRAD) Ocean Surface Wind Observations for Determining Tropical Storm Vortex Intensity and Structure

    Science.gov (United States)

    Atlas, Robert; Bailey, M. C.; Black, Peter; James, Mark; Johnson, James; Jones, Linwood; Miller, Timothy; Ruf, Christopher; Uhlhorn, Eric

    2008-01-01

    The Hurricane Imaging Radiometer (HIRAD) is an innovative technology development, which offers the potential of new and unique remotely sensed observations of both extreme oceanic wind events and strong precipitation from either UAS or satellite platforms. It is based on the airborne Stepped Frequency Microwave Radiometer (SFMR), which is a proven aircraft remote sensing technique for observing tropical cyclone ocean surface wind speeds and rain rates, including those of major hurricane intensity. The proposed HIRAD instrument advances beyond the current nadir viewing SFMR to an equivalent wide-swath SFMR imager using passive microwave synthetic thinned aperture radiometer technology. This sensor will operate over 4-7 GHz (C-band frequencies) where the required tropical cyclone remote sensing physics has been validated by both SFMR and WindSat radiometers. HIRAD incorporates a unique, technologically advanced array antenna and several other technologies successfully demonstrated by the NASA's Instrument Incubator Program. A brassboard version of the instrument is complete and has been successfully tested in an anechoic chamber, and development of the aircraft instrument is well underway. HIRAD will be a compact, lightweight, low-power instrument with no moving parts that will produce wide-swath imagery of ocean vector winds and rain during hurricane conditions when existing microwave sensors (radiometers or scatterometers) are hindered. Preliminary studies show that HIRAD will have a significant positive impact on analyses as either a new aircraft or satellite sensor.

  12. Antibody mimetics: promising complementary agents to animal-sourced antibodies.

    Science.gov (United States)

    Baloch, Abdul Rasheed; Baloch, Abdul Wahid; Sutton, Brian J; Zhang, Xiaoying

    2016-01-01

    Despite their wide use as therapeutic, diagnostic and detection agents, the limitations of polyclonal and monoclonal antibodies have inspired scientists to design the next generation biomedical agents, so-called antibody mimetics that offer many advantages over conventional antibodies. Antibody mimetics can be constructed by protein-directed evolution or fusion of complementarity-determining regions through intervening framework regions. Substantial progress in exploiting human, butterfly (Pieris brassicae) and bacterial systems to design and select mimetics using display technologies has been made in the past 10 years, and one of these mimetics [Kalbitor® (Dyax)] has made its way to market. Many challenges lie ahead to develop mimetics for various biomedical applications, especially those for which conventional antibodies are ineffective, and this review describes the current characteristics, construction and applications of antibody mimetics compared to animal-sourced antibodies. The possible limitations of mimetics and future perspectives are also discussed.

  13. Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability

    Directory of Open Access Journals (Sweden)

    Fleur S. van de Bovenkamp

    2018-04-01

    Full Text Available Immunoglobulin G (IgG can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains. These Fab glycans are acquired following introduction of N-glycosylation sites during somatic hypermutation and contribute to antibody diversification. We investigated whether Fab glycans may—in addition to affecting antigen binding—contribute to antibody stability. By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability. Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies. Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability. These findings indicate that introducing Fab glycans may represent a mechanism to improve therapeutic/diagnostic antibody stability.

  14. Clinical use of antibodies

    International Nuclear Information System (INIS)

    Baum, R.P.; Hoer, Gustav; Cox, P.H.; Buraggi, G.L.

    1991-01-01

    Use of monoclonal antibodies as tumour specific carrier molecules for therapeutic agents or as in vivo diagnostic reagents when labelled with radionuclides or NMR signal enhancers is attracting more and more attention. The potential is enormous but the technical problems are also considerable requiring the concerted action of many different scientific disciplines. This volume is based upon a symposium organised in Frankfurt in 1990 under the auspices of the European Association of Nuclear Medicines' Specialist Task Groups on Cardiology and the Utility of Labelled Antibodies. It gives a multidisciplinary review of the state of the art and of problems to be solved as well as recording the not inconsiderable successes which have been booked to date. The book will be of value as a reference to both clinicians and research scientists. refs.; figs.; tabs

  15. Delta antibody radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Kselikova, M; Urbankova, J

    1985-11-15

    The principle and procedure are described of the radioimmunoassay of delta antibody (delta-Ab) using the ABBOTT ANTI-DELTA kit by Abbott Co. A description is given of the kit, the working procedure and the method of evaluation. The results are reported of the incidence of delta-Ab in sera of patients with viral hepatitis B, in haemophiliacs, carriers of the hepatitis B virus surface antigen (HBsAg) and blood donors. The presence was detected of delta-Ab in one HBsAg carrier. The necessity is emphasized of delta-Ab determinations in the blood of donors in view of the antibody transfer with blood and blood preparations.

  16. [Antibody therapy for Alzheimer's disease].

    Science.gov (United States)

    Tabira, Takeshi; Matsumoto, Shin-Ei; Jin, Haifeng

    2011-11-01

    In order to avoid Abeta-induced autoimmune encephalitis, several monoclonal and polyclonal antibodies are in clinical trials. These are bapineuzumab, solanezumab, ponezumab, gantenerumab, BAN2401, gammaguard and octagam. Since each antibody has a different antigen epitope of Abeta, anti-amyloid activities are different. It is unknown which antibody is effective for Alzheimer disease, and we must wait for the result of clinical trials. Some patients who developed tissue amyloid plaque immuno-reactive (TAPIR) antibody showed slower decline after AN-1792 vaccination. We developed TAPIR-like monoclonal antibody, which was found to react with Abeta oligomers preferentially.

  17. Island based radar and microwave radiometer measurements of stratus cloud parameters during the Atlantic Stratocumulus Transition Experiment (ASTEX)

    Energy Technology Data Exchange (ETDEWEB)

    Frisch, A.S. [Colorado State Univ., Fort Collins, CO (United States); Fairall, C.W.; Snider, J.B. [NOAA Environmental Technology Lab., Boulder, CO (United States); Lenshow, D.H.; Mayer, S.D. [National Center for Atmospheric Research, Boulder, CO (United States)

    1996-04-01

    During the Atlantic Stratocumulus Transition Experiment (ASTEX) in June 1992, simultaneous measurements were made with a vertically pointing cloud sensing radar and a microwave radiometer. The radar measurements are used to estimate stratus cloud drizzle and turbulence parameters. In addition, with the microwave radiometer measurements of reflectivity, we estimated the profiles of cloud liquid water and effective radius. We used radar data for computation of vertical profiles of various drizzle parameters such as droplet concentration, modal radius, and spread. A sample of these results is shown in Figure 1. In addition, in non-drizzle clouds, with the radar and radiometer we can estimate the verticle profiles of stratus cloud parameters such as liquid water concentration and effective radius. This is accomplished by assuming a droplet distribution with droplet number concentration and width constant with height.

  18. The Effect of Atmospheric Scattering as Inferred from the Rocket-Borne UV Radiometer Measurements

    Directory of Open Access Journals (Sweden)

    Jhoon Kim

    1997-06-01

    Full Text Available Radiometers in UV and visible wavelengths were onboard the Korean Sounding Rocket(KSR-1 and 2 which were launched on June 4th and September 1st, 1993. These radiometers were designed to capture the solar radiation during the ascending period of the rocket flight. The purpose of the instrument was to measure the vertical profiles of stratospheric ozone densities. Since the instrument measured the solar radiation from the ground to its apogee, it is possible to investigate the altitude variation of the measured intensity and to estimate the effect of atmospheric scattering by comparing the UV and visible intensity. The visible channel was a reference because the 450-nm wavelength is in the atmospheric window region, where the solar radiation is transmitted through the atmosphere without being absorbed by other atmospheric gases. The use of 450-nm channel intensity as a reference should be limited to the altitude ranges above the certain altitudes, say 20 to 25§° where the signals are not perturbed by atmospheric scattering effects.

  19. Microwave Radiometers for Fire Detection in Trains: Theory and Feasibility Study

    Directory of Open Access Journals (Sweden)

    Federico Alimenti

    2016-06-01

    Full Text Available This paper introduces the theory of fire detection in moving vehicles by microwave radiometers. The system analysis is discussed and a feasibility study is illustrated on the basis of two implementation hypotheses. The basic idea is to have a fixed radiometer and to look inside the glass windows of the wagon when it passes in front of the instrument antenna. The proposed sensor uses a three-pixel multi-beam configuration that allows an image to be formed by the movement of the train itself. Each pixel is constituted by a direct amplification microwave receiver operating at 31.4 GHz. At this frequency, the antenna can be a 34 cm offset parabolic dish, whereas a 1 K brightness temperature resolution is achievable with an overall system noise figure of 6 dB, an observation bandwidth of 2 GHz and an integration time of 1 ms. The effect of the detector noise is also investigated and several implementation hypotheses are discussed. The presented study is important since it could be applied to the automatic fire alarm in trains and moving vehicles with dielectric wall/windows.

  20. Results from the Fourth WMO Filter Radiometer Comparison for aerosol optical depth measurements

    Science.gov (United States)

    Kazadzis, Stelios; Kouremeti, Natalia; Diémoz, Henri; Gröbner, Julian; Forgan, Bruce W.; Campanelli, Monica; Estellés, Victor; Lantz, Kathleen; Michalsky, Joseph; Carlund, Thomas; Cuevas, Emilio; Toledano, Carlos; Becker, Ralf; Nyeki, Stephan; Kosmopoulos, Panagiotis G.; Tatsiankou, Viktar; Vuilleumier, Laurent; Denn, Frederick M.; Ohkawara, Nozomu; Ijima, Osamu; Goloub, Philippe; Raptis, Panagiotis I.; Milner, Michael; Behrens, Klaus; Barreto, Africa; Martucci, Giovanni; Hall, Emiel; Wendell, James; Fabbri, Bryan E.; Wehrli, Christoph

    2018-03-01

    This study presents the results of the Fourth Filter Radiometer Comparison that was held in Davos, Switzerland, between 28 September and 16 October 2015. Thirty filter radiometers and spectroradiometers from 12 countries participated including reference instruments from global aerosol networks. The absolute differences of all instruments compared to the reference have been based on the World Meteorological Organization (WMO) criterion defined as follows: 95% of the measured data has to be within 0.005 ± 0.001/m (where m is the air mass). At least 24 out of 29 instruments achieved this goal at both 500 and 865 nm, while 12 out of 17 and 13 out of 21 achieved this at 368 and 412 nm, respectively. While searching for sources of differences among different instruments, it was found that all individual differences linked to Rayleigh, NO2, ozone, water vapor calculations and related optical depths and air mass calculations were smaller than 0.01 in aerosol optical depth (AOD) at 500 and 865 nm. Different cloud-detecting algorithms used have been compared. Ångström exponent calculations showed relatively large differences among different instruments, partly because of the high calculation uncertainty of this parameter in low AOD conditions. The overall low deviations of these AOD results and the high accuracy of reference aerosol network instruments demonstrated a promising framework to achieve homogeneity, compatibility and harmonization among the different spectral AOD networks in the near future.

  1. Development of electronics and data acquisition system for independent calibration of electron cyclotron emission radiometer

    Energy Technology Data Exchange (ETDEWEB)

    Kumari, Praveena, E-mail: praveena@ipr.res.in; Raulji, Vismaysinh; Mandaliya, Hitesh; Patel, Jignesh; Siju, Varsha; Pathak, S.K.; Rajpal, Rachana; Jha, R.

    2016-11-15

    Highlights: • Indigenous development of an electronics and data acquisition system to digitize signals for a desired time and automatization of calibration process. • 16 bit DAQ board with form factor of 90 × 89 mm. • VHDL Codes written for generating control signals for PC104 Bus, ADC and RAM. • Averaging process is done in two ways single point averaging and additive averaging. - Abstract: Signal conditioning units (SCU) along with Multichannel Data acquisition system (DAS) are developed and installed for automatization and frequent requirement of absolute calibration of ECE radiometer system. The DAS is an indigenously developed economical system which is based on Single Board Computer (SBC). The onboard RAM memory of 64 K for each channel enables the DAS for simultaneous and continuous acquisition. A Labview based graphical user interface provides commands locally or remotely to acquire, process, plot and finally save the data in binary format. The microscopic signals received from radiometer are strengthened, filtered by SCU and acquired through DAS for the set time and at set sampling frequency. Stored data are processed and analyzed offline with Labview utility. The calibration process has been performed for two hours continuously at different sampling frequency (100 Hz to 1 KHz) at two set of temperature like hot body and the room temperature. The detailed hardware and software design and testing results are explained in the paper.

  2. Hurricane Imaging Radiometer (HIRAD) Wind Speed Retrievals and Assessment Using Dropsondes

    Science.gov (United States)

    Cecil, Daniel J.; Biswas, Sayak K.

    2018-01-01

    The Hurricane Imaging Radiometer (HIRAD) is an experimental C-band passive microwave radiometer designed to map the horizontal structure of surface wind speed fields in hurricanes. New data processing and customized retrieval approaches were developed after the 2015 Tropical Cyclone Intensity (TCI) experiment, which featured flights over Hurricanes Patricia, Joaquin, Marty, and the remnants of Tropical Storm Erika. These new approaches produced maps of surface wind speed that looked more realistic than those from previous campaigns. Dropsondes from the High Definition Sounding System (HDSS) that was flown with HIRAD on a WB-57 high altitude aircraft in TCI were used to assess the quality of the HIRAD wind speed retrievals. The root mean square difference between HIRAD-retrieved surface wind speeds and dropsonde-estimated surface wind speeds was 6.0 meters per second. The largest differences between HIRAD and dropsonde winds were from data points where storm motion during dropsonde descent compromised the validity of the comparisons. Accounting for this and for uncertainty in the dropsonde measurements themselves, we estimate the root mean square error for the HIRAD retrievals as around 4.7 meters per second. Prior to the 2015 TCI experiment, HIRAD had previously flown on the WB-57 for missions across Hurricanes Gonzalo (2014), Earl (2010), and Karl (2010). Configuration of the instrument was not identical to the 2015 flights, but the methods devised after the 2015 flights may be applied to that previous data in an attempt to improve retrievals from those cases.

  3. Hurricane Imaging Radiometer Wind Speed and Rain Rate Retrievals during the 2010 GRIP Flight Experiment

    Science.gov (United States)

    Sahawneh, Saleem; Farrar, Spencer; Johnson, James; Jones, W. Linwood; Roberts, Jason; Biswas, Sayak; Cecil, Daniel

    2014-01-01

    Microwave remote sensing observations of hurricanes, from NOAA and USAF hurricane surveillance aircraft, provide vital data for hurricane research and operations, for forecasting the intensity and track of tropical storms. The current operational standard for hurricane wind speed and rain rate measurements is the Stepped Frequency Microwave Radiometer (SFMR), which is a nadir viewing passive microwave airborne remote sensor. The Hurricane Imaging Radiometer, HIRAD, will extend the nadir viewing SFMR capability to provide wide swath images of wind speed and rain rate, while flying on a high altitude aircraft. HIRAD was first flown in the Genesis and Rapid Intensification Processes, GRIP, NASA hurricane field experiment in 2010. This paper reports on geophysical retrieval results and provides hurricane images from GRIP flights. An overview of the HIRAD instrument and the radiative transfer theory based, wind speed/rain rate retrieval algorithm is included. Results are presented for hurricane wind speed and rain rate for Earl and Karl, with comparison to collocated SFMR retrievals and WP3D Fuselage Radar images for validation purposes.

  4. Capabilities and Impact on Wind Analyses of the Hurricane Imaging Radiometer (HIRAD)

    Science.gov (United States)

    Miller, Timothy L.; Amarin, Ruba; Atlas, Robert; Bailey, M. C.; Black, Peter; Buckley, Courtney; James, Mark; Johnson, James; Jones, Linwood; Ruf, Christopher; hide

    2010-01-01

    The Hurricane Imaging Radiometer (HIRAD) is a new airborne microwave remote sensor for hurricane observations that is currently under development by NASA Marshall Space Flight Center in partnership with the NOAA Atlantic Oceanographic and Meteorological Laboratory/Hurricane Research Division, the University of Central Florida, the University of Michigan, and the University of Alabama in Huntsville. The instrument is being test flown in January and is expected to participate in or collaborate with the tropical cyclone experiment GRIP (Genesis and Rapid Intensification Processes) in the 2010 season. HIRAD is designed to study the wind field in some detail within strong hurricanes and to enhance the real-time airborne ocean surface winds observation capabilities of NOAA and USAF Weather Squadron hurricane hunter aircraft currently using the operational Stepped Frequency Microwave Radiometer (SFMR). Unlike SFMR, which measures wind speed and rain rate along the ground track at a single point directly beneath the aircraft, HIRAD will provide images of the surface wind and rain field over a wide swath (approx.3 x the aircraft altitude) with approx.2 km resolution. See Figure 1, which depicts a simulated HIRAD swath versus the line of data obtained by SFMR.

  5. The advanced along track scanning radiometer (aatsr) on esa's envisat satellite - an early assessment

    Science.gov (United States)

    Llewellyn-Jones, D.; Mutlow, C.; Smith, D.; Edwards, M.

    The AATSR sensor is an imaging radiometer designed to measure top-of-the- atmosphere brightness temperature in seven thermal infrared, reflected infrared and visible wavelength channels. The main objective of the AATSR mission is to generate fields of global sea-surface temperature to the high levels of accuracy required for the monitoring and detection of climate change, and to support a broad range of associated research into the marine, terrestrial, cryospheric and atmospheric environments. An essential component of this objective is maintain continuity with the high-quality data-sets already collected form the two predecessor sensors, ATSR1 and 2 on ESA's ERS-1 and -2 satellites respectively. Following the successful launch of ENVISAT on March 1 2002, the AATSR sensor was activated and systematically brought up to full operating configuration in accordance with the agreed Switch-On and Data Acquisition Plan (SODAP). The early images form AATSR are of a quality that is consistent with its objective of effective data continuity. Since the instrument has been returning data, a programme of quality assessment has been taking place. This has included a systematic assessment of instrumental aspects such as signal-to-noise performance and image stability as well as the initial observations in the AATSR validation programme. In this programme, AATSR data-products are compared with correlative observations from other sources, which include, sea-borne radiometers, meteorological analysis fields and data from other satellites. This paper reports early results from some of the activities.

  6. Precision, accuracy and linearity of radiometer EML 105 whole blood metabolite biosensors.

    Science.gov (United States)

    Cobbaert, C; Morales, C; van Fessem, M; Kemperman, H

    1999-11-01

    The analytical performance of a new, whole blood glucose and lactate electrode system (EML 105 analyser. Radiometer Medical A/S. Copenhagen, Denmark) was evaluated. Between-day coefficients of variation were glucose and lactate, respectively. Recoveries of glucose were 100 +/- 10% using either aqueous or protein-based standards. Recoveries of lactate depended on the matrix, being underestimated in aqueous standards (approximately -10%) and 95-100% in standards containing 40 g/L albumin at lactate concentrations of 15 and 30 mmol/L. However, recoveries were high (up to 180%) at low lactate concentrations in protein-based standards. Carry-over, investigated according to National Clinical Chemistry Laboratory Standards EP10-T2, was negligible (alpha = 0.01). Glucose and lactate biosensors equipped with new membranes were linear up to 60 and 30 mmol/L, respectively. However, linearity fell upon daily use with increasing membrane lifetime. We conclude that the Radiometer metabolite biosensor results are reproducible and do not suffer from specimen-related carry-over. However, lactate recovery depends on the protein content and the lactate concentration.

  7. SU-E-I-14: Comparison of Iodine-Labeled and Indium-Labeled Antibody Biodistributions

    Energy Technology Data Exchange (ETDEWEB)

    Williams, L [Retired from City of Hope Medical Center, Arcadia, CA (United States)

    2014-06-01

    Purpose: It is often assumed that animal biodistributions of novel proteins are not dependent upon the radiolabel used in their determination. In units of percent injected dose per gram of tissue (%ID/g), organ uptake results (u) may be obtained using either iodine or metal as radioactive labels. Iodination is preferred as it is a one-step process whereas metal labeling requires two chemical procedures and therefore more protein material. It is important to test whether the radioactive tag leads to variation in the uptake value. Methods: Uptakes of 3antibodies to Carcinoembryonic Antigen (CEA) were evaluated in a nude mouse model bearing 150 to 300 mg LS174T human colon cancer xenografts. Antibodies included diabody (56 kDa), minibody (80kDa) and intact M5A (150 kDa) anti-CEA cognates. Both radioiodine and indium-111 labels were used with uptakes evaluated at 7 time(t) points out to 96 h. Ratios (R) of u(iodine-label)/u(indium-label) were determined for liver, spleen, kidneys, lung and tumor. Results: Hepatic loss was rapid for diabody and minibody; by 24 h their R values were only 2%; i.e., uptake of iodine was 2% of that of indium for these 2 antibodies. By contrast, R for the intact cognate was 50% at that time point. Splenic results were similar. Tumor uptake ratios did not depend upon the antibody type and were 50% at 24 h. Conclusions: Relatively rapid loss of iodine relative to indium in liver and spleen was observed in lower mass antibodies. Tumor ratios were larger and independent of antibody type. Aside from tumor, the R ratio of uptakes depended on the antibody type. R values decreased monotonically with time in all tissues and for all cognates. Using this ratio, one can possibly correct iodine-based u (t) results so that they resemble radiometal-derived biodistributions.

  8. Quantitative relationship between antibody affinity and antibody avidity

    International Nuclear Information System (INIS)

    Griswold, W.R.

    1987-01-01

    The relationship between antibody avidity, measured by the dissociation of the antigen-antibody bond in antigen excess, and antibody affinity was studied. Complexes of radiolabelled antigen and antibody of known affinity were prepared in vitro and allowed to stand for seven days to reach equilibrium. Then nonlabelled antigen in one hundred fold excess was added to dissociate the complexes. After an appropriate incubation the fraction of antigen bound to antibody was measured by the ammonium sulfate precipitation method. The dissociation index was the fraction bound in the experimental sample divided by the fraction bound in the control. The correlation coefficient between the dissociation index and the antibody binding constant was 0.92 for early dissociation and 0.98 for late dissociation. The regression equation relating the binding constant to the dissociation index was K = 6.4(DI) + 6.25, where DI is the late dissociation index and K is the logarithm to the base 10 of the binding constant. There is a high correlation between avidity and affinity of antibody. Antibody affinity can be estimated from avidity data. The stability of antigen-antibody complexes can be predicted from antibody affinity

  9. Flower elliptical constellation of millimeter-wave radiometers for precipitating cloud monitoring at geostationary scale

    Science.gov (United States)

    Marzano, F. S.; Cimini, D.; Montopoli, M.; Rossi, T.; Mortari, D.; di Michele, S.; Bauer, P.

    2009-04-01

    Millimeter-wave observation of the atmospheric parameters is becoming an appealing goal within satellite radiometry applications. The major technological advantage of millimeter-wave (MMW) radiometers is the reduced size of the overall system, for given performances, with respect to microwave sensor. On the other hand, millimeter-wave sounding can exploit window frequencies and various gaseous absorption bands at 50/60 GHz, 118 GHz and 183 GHz. These bands can be used to estimate tropospheric temperature profiles, integrated water vapor and cloud liquid content and, using a differentia spectral mode, light rainfall and snowfall. Millimeter-wave radiometers, for given observation conditions, can also exhibit relatively small field-of-views (FOVs), of the order of some kilometers for low-Earth-orbit (LEO) satellites. However, the temporal resolution of LEO millimeter-wave system observations remains a major drawback with respect to the geostationary-Earth-orbit (GEO) satellites. An overpass every about 12 hours for a single LEO platform (conditioned to a sufficiently large swath of the scanning MMW radiometer) is usually too much when compared with the typical temporal scale variation of atmospheric fields. This feature cannot be improved by resorting to GEO platforms due to their high orbit altitude and consequent degradation of the MMW-sensor FOVs. A way to tackle this impasse is to draw our attention at the regional scale and to focus non-circular orbits over the area of interest, exploiting the concept of micro-satellite flower constellations. The Flower Constellations (FCs) is a general class of elliptical orbits which can be optimized, through genetic algorithms, in order to maximize the revisiting time and the orbital height, ensuring also a repeating ground-track. The constellation concept nicely matches the choice of mini-satellites as a baseline choice, due to their small size, weight (less than 500 kilograms) and relatively low cost (essential when

  10. Assessment of Radiometer Calibration with GPS Radio Occultation for the MiRaTA CubeSat Mission

    Science.gov (United States)

    Marinan, Anne D.; Cahoy, Kerri L.; Bishop, Rebecca L.; Lui, Susan S.; Bardeen, James R.; Mulligan, Tamitha; Blackwell, William J.; Leslie, R. Vincent; Osaretin, Idahosa; Shields, Michael

    2017-01-01

    The Microwave Radiometer Technology Acceleration (MiRaTA) is a 3U CubeSat mission sponsored by the NASA Earth Science Technology Office (ESTO). The science payload on MiRaTA consists of a tri-band microwave radiometer and Global Positioning System (GPS) radio occultation (GPSRO) sensor. The microwave radiometer takes measurements of all-weather temperature (V-band, 50-57 GHz), water vapor (G-band, 175-191 GHz), and cloud ice (G-band, 205 GHz) to provide observations used to improve weather forecasting. The Aerospace Corporation's GPSRO experiment, called the Compact TEC (Total Electron Content) and Atmospheric GPS Sensor (CTAGS), measures profiles of temperature and pressure in the upper troposphere/lower stratosphere (∼20 km) and electron density in the ionosphere (over 100 km). The MiRaTA mission will validate new technologies in both passive microwave radiometry and GPS radio occultation: (1) new ultra-compact and low-power technology for multi-channel and multi-band passive microwave radiometers, (2) the application of a commercial off the shelf (COTS) GPS receiver and custom patch antenna array technology to obtain neutral atmospheric GPSRO retrieval from a nanosatellite, and (3) a new approach to spaceborne microwave radiometer calibration using adjacent GPSRO measurements. In this paper, we focus on objective (3), developing operational models to meet a mission goal of 100 concurrent radiometer and GPSRO measurements, and estimating the temperature measurement precision for the CTAGS instrument based on thermal noise. Based on an analysis of thermal noise of the CTAGS instrument, the expected temperature retrieval precision is between 0.17 K and 1.4 K, which supports the improvement of radiometric calibration to 0.25 K. PMID:28828144

  11. Application of solid-phase antibodies to radioimmunoassay

    International Nuclear Information System (INIS)

    McConway, M.G.; Chapman, R.S.

    1986-01-01

    Two types of polymeric microparticle, Dynospheres and reprecipitated acid-hydrolysed nylon 6/6, and two methods of activating these particles with either tresyl chloride or carbonyldiimidazole (CDI) prior to covalent linkage of antibodies were investigated with a view towards their respective adoption for the preparation of general solid-phase reagents for immunoassay applications. Activation of each particle and coupling of antibodies was rapid irrespective of the activator. CDI proved to be the activator of choice since it was cheap, less hazardous, more efficient and less pH dependent than tresyl chloride. Both types of microparticle remain buoyant during the RIA incubation periods and form stable pellets after centrifugation. In second antibody applications immobilisation of the first antibody occurs with a short incubation period of 30 min. Nylon microparticles have a higher antibody-coupling capacity and are the particles of choice in both first and second antibody applications. However, the nylon microparticles possess marginally higher non-specific binding characteristics. (Auth.)

  12. Mathematical analysis of dengue virus antibody dynamics

    Science.gov (United States)

    Perera, Sulanie; Perera, SSN

    2018-03-01

    Dengue is a mosquito borne viral disease causing over 390 million infections worldwide per annum. Even though information on how infection is controlled and eradicated from the body is lacking, antibodies are thought to play a major role in clearing the virus. In this paper, a non-linear conceptual dynamical model with humoral immune response and absorption effect has been proposed for primary dengue infection. We have included the absorption of pathogens into uninfected cells since this effect causes the virus density in the blood to decrease. The time delay that arises in the production of antibodies was accounted and is introduced through a continuous function. The basic reproduction number R0 is computed and a detailed stability analysis is done. Three equilibrium states, namely the infection free equilibrium, no immune equilibrium and the endemic equilibrium were identified and the existence and the stability conditions of these steady states were obtained. Numerical simulations proved the results that were obtained. By establishing the characteristic equation of the model at infection free equilibrium, it was observed that the infection free equilibrium is locally asymptotically stable if R0 1. Stability regions are identified for infection free equilibrium state with respect to the external variables and it is observed as the virus burst rate increases, the stability regions would decrease. These results implied that for higher virus burst rates, other conditions in the body must be strong enough to eliminate the disease completely from the host. The effect of time delay of antibody production on virus dynamics is discussed. It was seen that as the time delay in production of antibodies increases, the time for viral decline also increased. Also it was observed that the virus count goes to negligible levels within 7 - 14 days after the onset of symptoms as seen in dengue infections.

  13. ESTAR: The Electronically Scanned Thinned Array Radiometer for remote sensing measurement of soil moisture and ocean salinity

    Science.gov (United States)

    Swift, C. T.

    1993-01-01

    The product of a working group assembled to help define the science objectives and measurement requirements of a spaceborne L-band microwave radiometer devoted to remote sensing of surface soil moisture and sea surface salinity is presented. Remote sensing in this long-wavelength portion of the microwave spectrum requires large antennas in low-Earth orbit to achieve acceptable spatial resolution. The proposed radiometer, ESTAR, is unique in that it employs aperture synthesis to reduce the antenna area requirements for a space system.

  14. Lidar-Radiometer Inversion Code (LIRIC) for the Retrieval of Vertical Aerosol Properties from Combined Lidar Radiometer Data: Development and Distribution in EARLINET

    Science.gov (United States)

    Chaikovsky, A.; Dubovik, O.; Holben, Brent N.; Bril, A.; Goloub, P.; Tanre, D.; Pappalardo, G.; Wandinger, U.; Chaikovskaya, L.; Denisov, S.; hide

    2015-01-01

    This paper presents a detailed description of LIRIC (LIdar-Radiometer Inversion Code)algorithm for simultaneous processing of coincident lidar and radiometric (sun photometric) observations for the retrieval of the aerosol concentration vertical profiles. As the lidar radiometric input data we use measurements from European Aerosol Re-search Lidar Network (EARLINET) lidars and collocated sun-photometers of Aerosol Robotic Network (AERONET). The LIRIC data processing provides sequential inversion of the combined lidar and radiometric data by the estimations of column-integrated aerosol parameters from radiometric measurements followed by the retrieval of height-dependent concentrations of fine and coarse aerosols from lidar signals using integrated column characteristics of aerosol layer as a priori constraints. The use of polarized lidar observations allows us to discriminate between spherical and non-spherical particles of the coarse aerosol mode. The LIRIC software package was implemented and tested at a number of EARLINET stations. Inter-comparison of the LIRIC-based aerosol retrievals was performed for the observations by seven EARLNET lidars in Leipzig, Germany on 25 May 2009. We found close agreement between the aerosol parameters derived from different lidars that supports high robustness of the LIRIC algorithm. The sensitivity of the retrieval results to the possible reduction of the available observation data is also discussed.

  15. [Study of anti-idiotype antibodies to human monoclonal antibody].

    Science.gov (United States)

    Harada, R; Takahashi, N; Owaki, I; Kannagi, R; Endo, N; Morita, N; Inoue, M

    1992-02-01

    A human monoclonal antibody, ll-50 (IgM, lambda), was generated, which reacted specifically with a major of glycolipid present in LS174T colon cancer cells. The glycolipid antigen which reacted with the ll-50 antibody was expected to four sugar residues from its TLC mobility, and it was ascertained that the glycolipid antigen which reacted with ll-50 antibody might be Lc4 antigen [Gal beta 1----3 GLcNAc beta 1----3 Gal beta 1----4 Glc beta 1----1 Cer] judging from TLC immunostaining and ELISA when the reactivity of ll-50 antibody was tested using various pure glycolipids in 3-5 sugar residues as an antigen. Sera in patients with malignant disorders and healthy individuals were analyzed by Sandwich assay of immobilized and biotinylated ll-50 antibody. The serum of the Lc4 antigen recognized by ll-50 antibody was significantly higher in patients with malignant disorders than that in healthy individuals (p less than 0.05). Three mouse monoclonal anti-idiotype antibodies, G3, B3 and C5 (all IgG1), were generated by the immunization of BALB/c mice with ll-50 antibody. These anti-idiotype antibodies specifically bound to to human monoclonal antibody, ll-50 and had a significant inhibitory activity towards the binding of ll-50 antibody to the Lc4 antigen. This indicated that these anti-idiotype antibodies, G3, B3, and C5, were paratope-related anti-idiotype antibodies. G3, B3, and C5 were expected to define the nearest idiotope because they could mutually inhibit ll-50 antibody. Sera in patients with malignant disorders and healthy individuals were analyzed by Sandwich assay of immobilized and biotinylated anti-idiotype antibodies, G3, B3, and C5. As to the ll-50 like antibodies defined by C5 (Id-C5+), the mean serum level in patients with malignant disorders was significantly higher than that in healthy individuals (p less than 0.05). As to the ll-50 like antibodies defined by B3 (Id-B3+), the mean serum level in patients with malignant disorders was significantly higher

  16. Biodistribution and tumor imaging of an anti-CEA single-chain antibody-albumin fusion protein

    International Nuclear Information System (INIS)

    Yazaki, Paul J.; Kassa, Thewodros; Cheung, Chia-wei; Crow, Desiree M.; Sherman, Mark A.; Bading, James R.; Anderson, Anne-Line J.; Colcher, David; Raubitschek, Andrew

    2008-01-01

    Albumin fusion proteins have demonstrated the ability to prolong the in vivo half-life of small therapeutic proteins/peptides in the circulation and thereby potentially increase their therapeutic efficacy. To evaluate if this format can be employed for antibody-based imaging, an anticarcinoembryonic antigen (CEA) single-chain antibody(scFv)-albumin fusion protein was designed, expressed and radiolabeled for biodistribution and imaging studies in athymic mice bearing human colorectal carcinoma LS-174T xenografts. The [ 125 I]-T84.66 fusion protein demonstrated rapid tumor uptake of 12.3% injected dose per gram (ID/g) at 4 h that reached a plateau of 22.7% ID/g by 18 h. This was a dramatic increase in tumor uptake compared to 4.9% ID/g for the scFv alone. The radiometal [ 111 In]-labeled version resulted in higher tumor uptake, 37.2% ID/g at 18 h, which persisted at the tumor site with tumor: blood ratios reaching 18:1 and with normal tissues showing limited uptake. Based on these favorable imaging properties, a pilot [ 64 Cu]-positron emission tomography imaging study was performed with promising results. The anti-CEA T84.66 scFv-albumin fusion protein demonstrates highly specific tumor uptake that is comparable to cognate recombinant antibody fragments. The radiometal-labeled version, which shows lower normal tissue accumulation than these recombinant antibodies, provides a promising and novel platform for antibody-based imaging agents

  17. Microbials for the production of monoclonal antibodies and antibody fragments.

    Science.gov (United States)

    Spadiut, Oliver; Capone, Simona; Krainer, Florian; Glieder, Anton; Herwig, Christoph

    2014-01-01

    Monoclonal antibodies (mAbs) and antibody fragments represent the most important biopharmaceutical products today. Because full length antibodies are glycosylated, mammalian cells, which allow human-like N-glycosylation, are currently used for their production. However, mammalian cells have several drawbacks when it comes to bioprocessing and scale-up, resulting in long processing times and elevated costs. By contrast, antibody fragments, that are not glycosylated but still exhibit antigen binding properties, can be produced in microbial organisms, which are easy to manipulate and cultivate. In this review, we summarize recent advances in the expression systems, strain engineering, and production processes for the three main microbials used in antibody and antibody fragment production, namely Saccharomyces cerevisiae, Pichia pastoris, and Escherichia coli. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Radioimmunoassay with heterologous antibody (hetero-antibody RIA)

    International Nuclear Information System (INIS)

    Iwasawa, Atsushi; Hayashi, Hiroaki; Itoh, Zen; Wakabayashi, Katsumi

    1991-01-01

    To develop a homologous radioimmunoassay (RIA) for a hormone of a small or rare animal often meets difficulty in collecting a large amount of purified antigen required for antibody production. On the other hand, to employ a heterologous RIA to estimate the hormone often gives poor sensitivity. To overcome this difficulty, a 'hetero-antibody' RIA was studied. In a hetero-antibody RIA system, a purified preparation of a hormone is used for radioiodination and standardization and a heterologous antibody to the hormone is used for the first antibody. Canine motilin and rat LH were selected as examples, and anti-porcine motilin and anti-hCG, anti-hCGβ or anti-ovine LHβ was used as the heterologous antibody. The sensitivities of the hetero-antibody RIAs were much higher than those of heterologous RIAs in any case, showing that these hetero-antibody RIA systems were suitable for practical use. To clarify the principle of hetero-antibody RIA, antiserum to porcine motilin was fractionated on an affinity column where canine motilin was immobilized. The fraction bound had greater constants of affinity with both porcine and canine motilins than the rest of the antibody fractions. This fraction also reacted with a synthetic peptide corresponding to the C-terminal sequence common to porcine and canine motilins in a competitive binding test with labeled canine motilin. These results suggest that an antibody population having high affinity and cross-reactivity is present in polyclonal antiserum and indicate that the population can be used in hetero-antibody RIA at an appropriate concentration. (author)

  19. One-dimensional stable distributions

    CERN Document Server

    Zolotarev, V M

    1986-01-01

    This is the first book specifically devoted to a systematic exposition of the essential facts known about the properties of stable distributions. In addition to its main focus on the analytic properties of stable laws, the book also includes examples of the occurrence of stable distributions in applied problems and a chapter on the problem of statistical estimation of the parameters determining stable laws. A valuable feature of the book is the author's use of several formally different ways of expressing characteristic functions corresponding to these laws.

  20. Human antibody technology and the development of antibodies against cytomegalovirus.

    Science.gov (United States)

    Ohlin, Mats; Söderberg-Nauclér, Cecilia

    2015-10-01

    Cytomegalovirus (CMV) is a virus that causes chronic infections in a large set of the population. It may cause severe disease in immunocompromised individuals, is linked to immunosenescence and implied to play an important role in the pathogenesis of cardiovascular diseases and cancer. Modulation of the immune system's abilities to manage the virus represent a highly viable therapeutic option and passive immunotherapy with polyclonal antibody preparations is already in clinical use. Defined monoclonal antibodies offer many advantages over polyclonal antibodies purified from serum. Human CMV-specific monoclonal antibodies have consequently been thoroughly investigated with respect to their potential in the treatment of diseases caused by CMV. Recent advances in human antibody technology have substantially expanded the breadth of antibodies for such applications. This review summarizes the fundamental basis for treating CMV disease by use of antibodies, the basic technologies to be used to develop such antibodies, and relevant human antibody specificities available to target this virus. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Production of Y-86 and other radiometals for research purposes using a solution target system

    International Nuclear Information System (INIS)

    Oehlke, Elisabeth; Hoehr, Cornelia; Hou, Xinchi; Hanemaayer, Victoire; Zeisler, Stefan; Adam, Michael J.; Ruth, Thomas J.; Celler, Anna; Buckley, Ken; Benard, Francois; Schaffer, Paul

    2015-01-01

    Introduction: Diagnostic radiometals are typically obtained from cyclotrons by irradiating solid targets or from radioisotope generators. These methods have the advantage of high production yields, but require additional solid target handling infrastructure that is not readily available to many cyclotron facilities. Herein, we provide an overview of our results regarding the production of various positron-emitting radiometals using a liquid target system installed on a 13 MeV cyclotron at TRIUMF. Details about the production, purification and quality control of 89 Zr, 68 Ga and for the first time 86 Y are discussed. Methods: Aqueous solutions containing 1.35–1.65 g/mL of natural-abundance zinc nitrate, yttrium nitrate, and strontium nitrate were irradiated on a 13 MeV cyclotron using a standard liquid target. Different target body and foil materials were investigated for corrosion. Production yields were calculated using theoretical cross-sections from the EMPIRE code and compared with experimental results. The radioisotopes were extracted from irradiated target material using solid phase extraction methods adapted from previously reported methods, and used for radiolabelling experiments. Results: We demonstrated production quantities that are sufficient for chemical and biological studies for three separate radiometals, 89 Zr (A sat = 360 MBq/μA and yield = 3.17 MBq/μA), 86 Y (A sat = 31 MBq/μA and yield = 1.44 MBq/μA), and 68 Ga (A sat = 141 MBq/μA and yield = 64 MBq/μA) from one hour long irradiations on a typical medical cyclotron. 68 Ga yields were sufficient for potential clinical applications. In order to avoid corrosion of the target body and target foil, nitrate solutions were chosen as well as niobium as target-body material. An automatic loading system enabled up to three production runs per day. The separation efficiency ranged from 82 to 99%. Subsequently, 68 Ga and 86 Y were successfully used to radiolabel DOTA-based chelators while

  2. Prelaunch Performance of the 118 GHz Polarcube 3U Cubesat Temperature Sounding Radiometer

    Science.gov (United States)

    Periasamy, L.; Gasiewski, A. J.; Gallaher, D. W.; Sanders, B. T.; Belter, R.; Kraft, D.; Castillo, J.; Gordon, J. A.; Hurowitz, M.

    2017-12-01

    The low cost PolarCube 3U CubeSat supports a 118.75 GHz imaging spectrometer for temperature profiling of the troposphere and surface temperature. It is a demonstrator for a constellation of LEO passive microwave sensors at V-band and other frequencies using 3U/6U CubeSats. Such a satellite constellation for weather forecasting will provide data at high spatial and temporal resolution to observe rapidly evolving mesoscale weather. The satellite's payload is an eight channel, double sideband passive microwave temperature sounder with cross-track scanning and will provide 18 km surface resolution from a 400 km orbit. The radiometer implements a two-point calibration using an internal PIN switch and view of cold space. Although the instrument is based on a well established classical design, the challenges lie in developing a sensitive spectrometer that fits in a 1.5U volume, is low cost, consumes 4 W power and satisfies the CubeSat weight and envelope constraints. PolarCube is scheduled for launch on a Virgin Galactic flight in summer, 2018. The estimated radiometer sensitivity, ΔTrms varies from 0.3 to 2 K across the eight channels. The 50 MHz to 7 GHz 8-channel filter bank (designed with surface mount capacitors and inductors) fits on a 9x5 cm2 RO4350B PCB and includes 2-stage amplification and detector circuitry. The scanning reflector with an 8 cm2 main aperture uses a 3D printed corrugated feed that includes a WR8 to WC8 waveguide transition with a 17° bend. Initial performance results from the instrument using the 3D printed feed and IF/VA board obtained from airborne measurements over Antarctica on the NASA DC8 in early November 2016 indicate a well-functioning radiometer. The end-to-end characterization of the payload with the satellite bus, performance results from vibration and thermal-vacuum tests and roof-top measurements will be presented.

  3. Tabhu: tools for antibody humanization

    DEFF Research Database (Denmark)

    Olimpieri, Pier Paolo; Marcatili, Paolo; Tramontano, Anna

    2015-01-01

    Antibodies are rapidly becoming essential tools in the clinical practice, given their ability to recognize their cognate antigens with high specificity and affinity, and a high yield at reasonable costs in model animals. Unfortunately, when administered to human patients, xenogeneic antibodies can...... elicit unwanted and dangerous immunogenic responses. Antibody humanization methods are designed to produce molecules with a better safety profile still maintaining their ability to bind the antigen. This can be accomplished by grafting the non-human regions determining the antigen specificity...... and time-consuming experiments. Here we present tools for antibody humanization (Tabhu) a web server for antibody humanization. Tabhu includes tools for human template selection, grafting, back-mutation evaluation, antibody modelling and structural analysis, helping the user in all the critical steps...

  4. Cancer imaging with radiolabeled antibodies

    International Nuclear Information System (INIS)

    Goldenberg, D.M.

    1990-01-01

    This book presents a perspective of the use of antibodies to target diagnostic isotopes to tumors. Antibodies with reasonable specificity can be developed against almost any substance. If selective targeting to cancer cells can be achieved, the prospects for a selective therapy are equally intriguing. But the development of cancer detection, or imaging, with radiolabeled antibodies has depended upon advances in a number of different areas, including cancer immunology and immunochemistry for identifying suitable antigen targets and antibodies to these targets, tumor biology for model systems, radiochemistry for he attachment of radionuclides to antibodies, molecular biology for reengineering the antibodies for safer and more effective use in humans, and nuclear medicine for providing the best imaging protocols and instrumentation to detect minute amounts of elevated radioactivity against a background of considerable noise. Accordingly, this book has been organized to address the advances that are being made in many of these areas

  5. Monoclonal antibodies for treating cancer

    International Nuclear Information System (INIS)

    Dillman, R.O.

    1989-01-01

    The purpose of this study is to assess the current status of in-vivo use of monoclonal antibodies for treating cancer. Publications appearing between 1980 and 1988 were identified by computer searches using MEDLINE and CANCERLIT, by reviewing the table of contents of recently published journals, and by searching bibliographies of identified books and articles. More than 700 articles, including peer-reviewed articles and book chapters, were identified and selected for analysis. The literature was reviewed and 235 articles were selected as relevant and representative of the current issues and future applications for in-vivo monoclonal antibodies for cancer therapy and of the toxicity and efficacy which has been associated with clinical trials. Approaches include using antibody alone (interacting with complement or effector cells or binding directly with certain cell receptors) and immunoconjugates (antibody coupled to radioisotopes, drugs, toxins, or other biologicals). Most experience has been with murine antibodies. Trials of antibody alone and radiolabeled antibodies have confirmed the feasibility of this approach and the in-vivo trafficking of antibodies to tumor cells. However, tumor cell heterogeneity, lack of cytotoxicity, and the development of human antimouse antibodies have limited clinical efficacy. Although the immunoconjugates are very promising, heterogeneity and the antimouse immune response have hampered this approach as has the additional challenge of chemically or genetically coupling antibody to cytotoxic agents. As a therapeutic modality, monoclonal antibodies are still promising but their general use will be delayed for several years. New approaches using human antibodies and reducing the human antiglobulin response should facilitate treatment. 235 references

  6. Stable isotope research pool inventory

    International Nuclear Information System (INIS)

    1980-12-01

    This report contains a listing of electromagnetically separated stable isotopes which are available for distribution within the United States for non-destructive research use from the Oak Ridge National Laboratory on a loan basis. This inventory includes all samples of stable isotopes in the Materials Research Collection and does not designate whether a sample is out on loan or in reprocessing

  7. Tumor imaging with monoclonal antibodies

    International Nuclear Information System (INIS)

    Haisma, H.; Hilgers, J.

    1987-01-01

    Many monoclonal antibodies directed against tumor-associated antigens have been identified, but so far none of these are tumor specific. Polyclonal and monoclonal antibodies have been used for imaging of a wide variety of tumors with success. Radiolabeling of antibody is usually done with iodine isotopes of which 123 I is the best candidate for radioimmunodetection purposes. The labeling of antibodies through chelates makes it possible to use metal radioisotopes like 111 In, which is the best radioisotope for imaging with monoclonal antibodies due to its favorable half-life of 2.5 days. Usually imaging cannot be performed within 24 h after injection, but clearance of antibody can be increased by using F(ab) 2 of Fab. Another approach is to clear non-bound antibody by a second antibody, directed against the first. The detection limit of immunoimaging is about 2 cm, but will be improved by tomography or SPECT. There is still a high false positive and false negative rate, which makes it impossible to use radioimmunodetection as the only technique for diagnosis of tumors. In combination with other detection techniques, tumor imaging with monoclonal antibodies can improve diagnosis. 44 refs.; 3 tabs

  8. Forest canopy height from Multiangle Imaging SpectroRadiometer (MISR) assessed with high resolution discrete return lidar

    Science.gov (United States)

    Mark Chopping; Anne Nolin; Gretchen G. Moisen; John V. Martonchik; Michael Bull

    2009-01-01

    In this study retrievals of forest canopy height were obtained through adjustment of a simple geometricoptical (GO) model against red band surface bidirectional reflectance estimates from NASA's Multiangle Imaging SpectroRadiometer (MISR), mapped to a 250 m grid. The soil-understory background contribution was partly isolated prior to inversion using regression...

  9. Soil Moisture Active Passive (SMAP) Microwave Radiometer Radio-Frequency Interference (RFI) Mitigation: Initial On-Orbit Results

    Science.gov (United States)

    Mohammed, Priscilla N.; Piepmeier, Jeffrey R.; Johnson, Joel T.; Aksoy, Mustafa; Bringer, Alexandra

    2015-01-01

    The Soil Moisture Active Passive (SMAP) mission, launched in January 2015, provides global measurements of soil moisture using a microwave radiometer. SMAPs radiometer passband lies within the passive frequency allocation. However, both unauthorized in-band transmitters as well as out-of-band emissions from transmitters operating at frequencies adjacent to this allocated spectrum have been documented as sources of radio frequency interference (RFI) to the L-band radiometers on SMOS and Aquarius. The spectral environment consists of high RFI levels as well as significant occurrences of low level RFI equivalent to 0.1 to 10 K. The SMAP ground processor reports the antenna temperature both before and after RFI mitigation is applied. The difference between these quantities represents the detected RFI level. The presentation will review the SMAP RFI detection and mitigation procedure and discuss early on-orbit RFI measurements from the SMAP radiometer. Assessments of global RFI properties and source types will be provided, as well as the implications of these results for SMAP soil moisture measurements.

  10. Retrieving soil moisture for non-forested areas using PALS radiometer measurements in SMAPVEX12 field campaign

    Science.gov (United States)

    In this paper we investigate retrieval of soil moisture based on L-band brightness temperature under diverse conditions and land cover types. We apply the PALS (Passive Active L-band System) radiometer data collected in the SMAPVEX12 (Soil Moisture Active Passive Validation Experiment 2012) field ex...

  11. A linear model to predict with a multi-spectral radiometer the amount of nitrogen in winter wheat

    NARCIS (Netherlands)

    Reyniers, M.; Walvoort, D.J.J.; Baardemaaker, De J.

    2006-01-01

    The objective was to develop an optimal vegetation index (VIopt) to predict with a multi-spectral radiometer nitrogen in wheat crop (kg[N] ha-1). Optimality means that nitrogen in the crop can be measured accurately in the field during the growing season. It also means that the measurements are

  12. Diagnostics of the SMOS radiometer antenna system at the DTU-ESA spherical near-field antenna test facility

    DEFF Research Database (Denmark)

    Cappellin, Cecilia; Frandsen, A.; Pivnenko, Sergey

    2007-01-01

    The recently developed Spherical Wave Expansion-to-Plane Wave Expansion (SWE-to-PWE) antenna diagnostics technique is employed in an investigation of the antenna system in the Microwave Imaging Radiometer using Aperture Synthesis (MIRAS) for ESA’s Soil Moisture and Ocean Salinity (SMOS) mission...

  13. Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens.

    OpenAIRE

    Jiménez, T; Díaz, A M; Zlotnik, H

    1990-01-01

    Nocardia asteroides and Nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (MAbs). Six stable hybrid cell lines secreting anti-Nocardia spp. MAbs were obtained. These were characterized by enzyme-linked immunosorbent assay, Western blot (immunoblot), and immunofluorescence assay. Although all the MAbs exhibited different degrees of cross-reactivity with N. asteroides and N. brasiliensis antigens as well as with culture-filtrate antigens from Myco...

  14. Research on Ground Motion Metal Target Based on Rocket Projectile by Using Millimeter Wave Radiometer Technology

    Directory of Open Access Journals (Sweden)

    Zhang Dongyang

    2014-06-01

    Full Text Available How to detect the ground motion metal target effectively is an important guarantee for precision strike in the process of Rocket Projectile flight. Accordingly and in view of the millimeter- wave radiation characteristic of the ground motion metal target, a mathematical model was established based on Rocket Projectile about millimeter-wave detection to the ground motion metal target. Through changing various parameters in the process of Rocket Projectile flight, the detection model was studied by simulation. The parameters variation and effective range of millimeter wave radiometer were obtained in the process of rotation and horizontal flight. So a certain theoretical basis was formed for the precision strike to the ground motion metal target.

  15. Reflectance of Antarctic surfaces from multispectral radiometers: The correction of atmospheric effects

    International Nuclear Information System (INIS)

    Zibordi, G.; Maracci, G.

    1993-01-01

    Monitoring reflectance of polar icecaps has relevance in climate studies. In fact, climate changes produce variations in the morphology of ice and snow covers, which are detectable as surface reflectance change. Surface reflectance can be retrieved from remotely sensed data. However, absolute values independent of atmospheric turbidity and surface altitude can only be obtained after removing masking effects of the atmosphere. An atmospheric correction model, accounting for surface and sensor altitudes above sea level, is described and validated through data detected over Antarctic surfaces with a Barnes Modular Multispectral Radiometer having bands overlapping those of the Landsat Thematic Mapper. The model is also applied in a sensitivity analysis to investigate error induced in reflectance obtained from satellite data by indeterminacy in optical parameters of atmospheric constituents. Results show that indeterminacy in the atmospheric water vapor optical thickness is the main source of nonaccuracy in the retrieval of surface reflectance from data remotely sensed over Antarctic regions

  16. Millimeter-wave Radiometer for High Sensitivity Water Vapor Profiling in Arid Regions

    Energy Technology Data Exchange (ETDEWEB)

    Pazmany, Andrew

    2006-11-09

    Abstract - ProSensing Inc. has developed a G-band (183 GHz) water Vapor Radiometer (GVR) for long-term, unattended measurements of low concentrations of atmospheric water vapor and liquid water. Precipitable water vapor and liquid water path are estimated from zenith brightness temperatures measured from four double-sideband receiver channels, centered at 183.31 1, 3 and 7, and 14 GHz. A prototype ground-based version of the instrument was deployed at the DOE ARM program?s North Slope of Alaska site near Barrow AK in April 2005, where it collected data continuously for one year. A compact, airborne version of this instrument, packaged to operate from a standard 2-D PMS probe canister, has been tested on the ground and is scheduled for test flights in the summer of 2006. This paper presents design details, laboratory test results and examples of retrieved precipitable water vapor and liquid water path from measured brightness temperature data.

  17. Diviner lunar radiometer observations of cold traps in the moon's south polar region

    Science.gov (United States)

    Paige, D.A.; Siegler, M.A.; Zhang, J.A.; Hayne, P.O.; Foote, E.J.; Bennett, K.A.; Vasavada, A.R.; Greenhagen, B.T.; Schofield, J.T.; McCleese, D.J.; Foote, M.C.; DeJong, E.; Bills, B.G.; Hartford, W.; Murray, B.C.; Allen, C.C.; Snook, K.; Soderblom, L.A.; Calcutt, S.; Taylor, F.W.; Bowles, N.E.; Bandfield, J.L.; Elphic, R.; Ghent, R.; Glotch, T.D.; Wyatt, M.B.; Lucey, P.G.

    2010-01-01

    Diviner Lunar Radiometer Experiment surface-temperature maps reveal the existence of widespread surface and near-surface cryogenic regions that extend beyond the boundaries of persistent shadow. The Lunar Crater Observation and Sensing Satellite (LCROSS) struck one of the coldest of these regions, where subsurface temperatures are estimated to be 38 kelvin. Large areas of the lunar polar regions are currently cold enough to cold-trap water ice as well as a range of both more volatile and less volatile species. The diverse mixture of water and high-volatility compounds detected in the LCROSS ejecta plume is strong evidence for the impact delivery and cold-trapping of volatiles derived from primitive outer solar system bodies.

  18. A Miniaturized Laser Heterodyne Radiometer for Greenhouse Gas Measurements in the Atmospheric Column

    Science.gov (United States)

    Steel, Emily Wilson

    2015-01-01

    Laser Heterodyne Radiometry is a technique adapted from radio receiver technology has been used to measure trace gases in the atmosphere since the 1960s.By leveraging advances in the telecommunications industry, it has been possible to miniaturize this technology.The mini-LHR (Miniaturized Laser Heterodyne Radiometer) has been under development at NASA Goddard Space flight Center since 2009. This sun-viewing instrument measures carbon dioxide and methane in the atmospheric column and operates in tandem with an AERONET sun photometer producing a simultaneous measure of aerosols. The mini-LHR has been extensively field tested in a range of locations ranging in the continental US as well as Alaska and Hawaii and now operates autonomously with sensitivities of approximately 0.2 ppmv and approximately10 ppbv, for carbon dioxide and methane respectively, for 10 averaged scans under clear sky conditions.

  19. Miniaturized Laser Heterodyne Radiometer for Measurements of CO2 in the Atmospheric Column

    Science.gov (United States)

    Wilson, E. L.; Mclinden, M. L.; Miller, J. H.; Allan, G. R.; Lott, L. E.; Melroy, H. R.; Clarke, G. B.

    2013-01-01

    We have developed a low-cost, miniaturized laser heterodyne radiometer for highly sensitive measurements of carbon dioxide (CO2) in the atmospheric column. In this passive design, sunlight that has undergone absorption by CO2 in the atmosphere is collected and mixed with continuous wave laser light that is step-scanned across the absorption feature centered at 1,573.6 nm. The resulting radio frequency beat signal is collected as a function of laser wavelength, from which the total column mole fraction can be de-convolved. We are expanding this technique to include methane (CH4) and carbon monoxide (CO), and with minor modifications, this technique can be expanded to include species such as water vapor (H2O) and nitrous oxide (N2O).

  20. Evaluation of the Delta-T SPN1 radiometer for the measurement of solar irradiance components

    Science.gov (United States)

    Estelles, Victor; Serrano, David; Segura, Sara; Wood, John; Webb, Nick; Utrillas, Maria Pilar

    2016-04-01

    In this study we analyse the performance of an SPN1 radiometer built by Delta-T Devices Ltd. to retrieve global solar irradiance at ground and its components (diffuse, direct) in comparison with measurements from two Kipp&Zonen CMP21 radiometers and a Kipp&Zonen CHP1 pirheliometer, mounted on an active Solys-2 suntracker at the Burjassot site (Valencia, Spain) using data acquired every minute during years 2013 - 2015. The measurement site is close to sea level (60 m a.s.l.), near the Mediterranean coast (10 km) and within the metropolitan area of Valencia City (over 1.500.000 inhabitants). The SPN1 is an inexpensive and versatile instrument for the measurement of the three components of the solar radiation without any mobile part and without any need to azimuthally align the instrument to track the sun (http://www.delta-t.co.uk). The three components of the solar radiation are estimated from a combination of measurements performed by 7 different miniature thermopiles. The SPN1 pyranometer measures the irradiance between 400 and 2700 nm, and the nominal uncertainty for the individual readings is about 8% ± 10 W/m2 (5% for the daily averages). The pyranometer Kipp&Zonen CMP21 model is a secondary standard for the measurement of broadband solar global irradiance in horizontal planes. Two ventilated CMP21 are used for the measurement of the global and diffuse irradiances. The expected total daily uncertainty of the radiometer is estimated to be 2%. The pirheliometer Kipp&Zonen CHP1 is designed for the measurement of the direct irradiance. The principles are similar to the CMP21 pyranometer. The results of the comparison show that the global irradiance from the SPN1 compares very well with the CMP21, with absolute RMSD and MBD differences below the combined uncertainties (15 W/m2 and -5.4 W/m2, respectively; relative RMSD of 3.1%). Both datasets are very well correlated, with a correlation coefficient higher than 0.997 and a slope and intercept very close to 1 and 0

  1. A Tissue Propagation Model for Validating Close-Proximity Biomedical Radiometer Measurements

    Science.gov (United States)

    Bonds, Q.; Herzig, P.; Weller, T.

    2016-01-01

    The propagation of thermally-generated electromagnetic emissions through stratified human tissue is studied herein using a non-coherent mathematical model. The model is developed to complement subsurface body temperature measurements performed using a close proximity microwave radiometer. The model takes into account losses and reflections as thermal emissions propagate through the body, before being emitted at the skin surface. The derivation is presented in four stages and applied to the human core phantom, a physical representation of a stomach volume of skin, muscle, and blood-fatty tissue. A drop in core body temperature is simulated via the human core phantom and the response of the propagation model is correlated to the radiometric measurement. The results are comparable, with differences on the order of 1.5 - 3%. Hence the plausibility of core body temperature extraction via close proximity radiometry is demonstrated, given that the electromagnetic characteristics of the stratified tissue layers are known.

  2. An optimal estimation algorithm to derive Ice and Ocean parameters from AMSR Microwave radiometer observations

    DEFF Research Database (Denmark)

    Pedersen, Leif Toudal; Tonboe, Rasmus T.; Høyer, Jacob

    channels as well as the combination of data from multiple sources such as microwave radiometry, scatterometry and numerical weather prediction. Optimal estimation is data assimilation without a numerical model for retrieving physical parameters from remote sensing using a multitude of available information......Global multispectral microwave radiometer measurements have been available for several decades. However, most current sea ice concentration algorithms still only takes advantage of a very limited subset of the available channels. Here we present a method that allows utilization of all available....... The methodology is observation driven and model innovation is limited to the translation between observation space and physical parameter space Over open water we use a semi-empirical radiative transfer model developed by Meissner & Wentz that estimates the multispectral AMSR brightness temperatures, i...

  3. Interpretation of the cosmic microwave background radiation anisotropy detected by the COBE Differential Microwave Radiometer

    Science.gov (United States)

    Wright, E. L.; Meyer, S. S.; Bennett, C. L.; Boggess, N. W.; Cheng, E. S.; Hauser, M. G.; Kogut, A.; Lineweaver, C.; Mather, J. C.; Smoot, G. F.

    1992-01-01

    The large-scale cosmic background anisotropy detected by the COBE Differential Microwave Radiometer (DMR) instrument is compared to the sensitive previous measurements on various angular scales, and to the predictions of a wide variety of models of structure formation driven by gravitational instability. The observed anisotropy is consistent with all previously measured upper limits and with a number of dynamical models of structure formation. For example, the data agree with an unbiased cold dark matter (CDM) model with H0 = 50 km/s Mpc and Delta-M/M = 1 in a 16 Mpc radius sphere. Other models, such as CDM plus massive neutrinos (hot dark matter (HDM)), or CDM with a nonzero cosmological constant are also consistent with the COBE detection and can provide the extra power seen on 5-10,000 km/s scales.

  4. A synthetic aperture microwave radiometer to measure soil moisture and ocean salinity from space

    Science.gov (United States)

    Le Vine, D. M.; Hilliard, L. M.; Swift, C. T.; Ruf, C. S.; Garrett, L. B.

    1991-01-01

    A concept is presented for a microwave radiometer in space to measure soil moisture and ocean salinity as part of an 'Earth Probe' mission. The measurements could be made using an array of stick antennas. The L-band channel (1.4 GHz) would be the primary channel for determining soil moisture, with the S-band (2.65-GHz) and C-band (5.0-GHz) channels providing ancillary information to help correct for the effects of the vegetation canopy and possibly to estimate a moisture profile. A preliminary study indicates that an orbit at 450 km would provide coverage of better than 95 percent of the earth every 3 days. A 10-km resolution cell (at nadir) requires stick antennas about 9.5-m long at L-band. The S-band and C-band sticks would be substantially shorter (5 m and 2.7 m, respectively).

  5. Implementation of Active Thermal Control (ATC) for the Soil Moisture Active and Passive (SMAP) Radiometer

    Science.gov (United States)

    Mikhaylov, Rebecca; Kwack, Eug; French, Richard; Dawson, Douglas; Hoffman, Pamela

    2014-01-01

    NASA's Earth Observing Soil Moisture Active and Passive (SMAP) Mission is scheduled to launch in November 2014 into a 685 kilometer near-polar, sun-synchronous orbit. SMAP will provide comprehensive global mapping measurements of soil moisture and freeze/thaw state in order to enhance understanding of the processes that link the water, energy, and carbon cycles. The primary objectives of SMAP are to improve worldwide weather and flood forecasting, enhance climate prediction, and refine drought and agriculture monitoring during its three year mission. The SMAP instrument architecture incorporates an L-band radar and an L-band radiometer which share a common feed horn and parabolic mesh reflector. The instrument rotates about the nadir axis at approximately 15 revolutions per minute, thereby providing a conically scanning wide swath antenna beam that is capable of achieving global coverage within three days. In order to make the necessary precise surface emission measurements from space, the electronics and hardware associated with the radiometer must meet tight short-term (instantaneous and orbital) and long-term (monthly and mission) thermal stabilities. Maintaining these tight thermal stabilities is quite challenging because the sensitive electronics are located on a fast spinning platform that can either be in full sunlight or total eclipse, thus exposing them to a highly transient environment. A passive design approach was first adopted early in the design cycle as a low-cost solution. With careful thermal design efforts to cocoon and protect all sensitive components, all stability requirements were met passively. Active thermal control (ATC) was later added after the instrument Preliminary Design Review (PDR) to mitigate the threat of undetected gain glitches, not for thermal-stability reasons. Gain glitches are common problems with radiometers during missions, and one simple way to avoid gain glitches is to use the in-flight set point programmability that ATC

  6. Application of automatic gain control for radiometer diagnostic in SST-1 tokamak.

    Science.gov (United States)

    Makwana, Foram R; Siju, Varsha; Edappala, Praveenlal; Pathak, S K

    2017-12-01

    This paper describes the characterisation of a negative feedback type of automatic gain control (AGC) circuit that will be an integral part of the heterodyne radiometer system operating at a frequency range of 75-86 GHz at SST-1 tokamak. The developed AGC circuit is a combination of variable gain amplifier and log amplifier which provides both gain and attenuation typically up to 15 dB and 45 dB, respectively, at a fixed set point voltage and it has been explored for the first time in tokamak radiometry application. The other important characteristics are that it exhibits a very fast response time of 390 ns to understand the fast dynamics of electron cyclotron emission and can operate at very wide input RF power dynamic range of around 60 dB that ensures signal level within the dynamic range of the detection system.

  7. Suomi National Polar-Orbiting Partnership Visible Infrared Imaging Radiometer Suite Polarization Sensitivity Analysis

    Science.gov (United States)

    Sun, Junqiang; Xiong, Xiaoxiong; Waluschka, Eugene; Wang, Menghua

    2016-01-01

    The Visible Infrared Imaging Radiometer Suite (VIIRS) is one of five instruments onboard the Suomi National Polar-Orbiting Partnership (SNPP) satellite that launched from Vandenberg Air Force Base, California, on October 28, 2011. It is a whiskbroom radiometer that provides +/-56.28deg scans of the Earth view. It has 22 bands, among which 14 are reflective solar bands (RSBs). The RSBs cover a wavelength range from 410 to 2250 nm. The RSBs of a remote sensor are usually sensitive to the polarization of incident light. For VIIRS, it is specified that the polarization factor should be smaller than 3% for 410 and 862 nm bands and 2.5% for other RSBs for the scan angle within +/-45deg. Several polarization sensitivity tests were performed prelaunch for SNPP VIIRS. The first few tests either had large uncertainty or were less reliable, while the last one was believed to provide the more accurate information about the polarization property of the instrument. In this paper, the measured data in the last polarization sensitivity test are analyzed, and the polarization factors and phase angles are derived from the measurements for all the RSBs. The derived polarization factors and phase angles are band, detector, and scan angle dependent. For near-infrared bands, they also depend on the half-angle mirror side. Nevertheless, the derived polarization factors are all within the specification, although the strong detector dependence of the polarization parameters was not expected. Compared to the Moderate Resolution Imaging Spectroradiometer on both Aqua and Terra satellites, the polarization effect on VIIRS RSB is much smaller.

  8. Upgrades and Real Time Ntm Control Application of the Ece Radiometer on Asdex Upgrade

    Science.gov (United States)

    Hicks, N. K.; Suttrop, W.; Behler, K.; Giannone, L.; Manini, A.; Maraschek, M.; Raupp, G.; Reich, M.; Sips, A. C. C.; Stober, J.; Treutterer, W.; ASDEX Upgrade Team; Cirant, S.

    2009-04-01

    The 60-channel electron cyclotron emission (ECE) radiometer diagnostic on the ASDEX Upgrade tokamak is presently being upgraded to include a 1 MHz sampling rate data acquisition system. This expanded capability allows electron temperature measurements up to 500 kHz (anti-aliasing filter cut-off) with spatial resolution ~1 cm, and will thus provide measurement of plasma phenomena on the MHD timescale, such as neoclassical tearing modes (NTMs). The upgraded and existing systems may be run in parallel for comparison, and some of the first plasma measurements using the two systems together are presented. A particular planned application of the upgraded radiometer is integration into a real-time NTM stabilization loop using targeted deposition of electron cyclotron resonance heating (ECRH). For this loop, it is necessary to determine the locations of the NTM and ECRH deposition using ECE measurements. As the magnetic island of the NTM repeatedly rotates through the ECE line of sight, electron temperature fluctuations at the NTM frequency are observed. The magnetic perturbation caused by the NTM is independently measured using Mirnov coils, and a correlation profile between these magnetic measurements and the ECE data is constructed. The phase difference between ECE oscillations on opposite sides of the island manifests as a zero-crossing of the correlation profile, which determines the NTM location in ECE channel space. To determine the location of ECRH power deposition, the power from a given gyrotron may be modulated at a particular frequency. Correlation analysis of this modulated signal and the ECE data identifies a particular ECE channel associated with the deposition of that gyrotron. Real time equilibrium reconstruction allows the ECE channels to be translated into flux surface and spatial coordinates for use in the feedback loop.

  9. Pre-Launch Calibration and Performance Study of the Polarcube 3u Temperature Sounding Radiometer Mission

    Science.gov (United States)

    Periasamy, L.; Gasiewski, A. J.; Sanders, B. T.; Rouw, C.; Alvarenga, G.; Gallaher, D. W.

    2016-12-01

    The positive impact of passive microwave observations of tropospheric temperature, water vapor and surface variables on short-term weather forecasts has been clearly demonstrated in recent forecast anomaly growth studies. The development of a fleet of such passive microwave sensors especially at V-band and higher frequencies in low earth orbit using 3U and 6U CubeSats could help accomplish the aforementioned objectives at low system cost and risk as well as provide for regularly updated radiometer technology. The University of Colorado's 3U CubeSat, PolarCube is intended to serve as a demonstrator for such a fleet of passive sounders and imagers. PolarCube supports MiniRad, an eight channel, double sideband 118.7503 GHz passive microwave sounder. The mission is focused primarily on sounding in Arctic and Antarctic regions with the following key remote sensing science and engineering objectives: (i) Collect coincident tropospheric temperature profiles above sea ice, open polar ocean, and partially open areas to develop joint sea ice concentration and lower tropospheric temperature mapping capabilities in clear and cloudy atmospheric conditions. This goal will be accomplished in conjunction with data from existing passive microwave sensors operating at complementary bands; and (ii) Assess the capabilities of small passive microwave satellite sensors for environmental monitoring in support of the future development of inexpensive Earth science missions. Performance data of the payload/spacecraft from pre-launch calibration will be presented. This will include- (i) characterization of the antenna sub-system comprising of an offset 3D printed feedhorn and spinning parabolic reflector and impact of the antenna efficiencies on radiometer performance, (ii) characterization of MiniRad's RF front-end and IF back-end with respect to temperature fluctuations and their impact on atmospheric temperature weighting functions and receiver sensitivity, (iii) results from roof

  10. 1D-Var temperature retrievals from microwave radiometer and convective scale model

    Directory of Open Access Journals (Sweden)

    Pauline Martinet

    2015-12-01

    Full Text Available This paper studies the potential of ground-based microwave radiometers (MWR for providing accurate temperature retrievals by combining convective scale numerical models and brightness temperatures (BTs. A one-dimensional variational (1D-Var retrieval technique has been tested to optimally combine MWR and 3-h forecasts from the French convective scale model AROME. A microwave profiler HATPRO (Humidity and Temperature PROfiler was operated during 6 months at the meteorological station of Bordeaux (Météo France. MWR BTs were monitored against simulations from the Atmospheric Radiative Transfer Simulator 2 radiative transfer model. An overall good agreement was found between observations and simulations for opaque V-band channels but large errors were observed for channels the most affected by liquid water and water vapour emissions (51.26 and 52.28 GHz. 1D-Var temperature retrievals are performed in clear-sky and cloudy conditions using a screening procedure based on cloud base height retrieval from ceilometer observations, infrared radiometer temperature and liquid water path derived from the MWR observations. The 1D-Var retrievals were found to improve the AROME forecasts up to 2 km with a maximum gain of approximately 50 % in root-mean-square-errors (RMSE below 500 m. They were also found to outperform neural network retrievals. A static bias correction was proposed to account for systematic instrumental errors. This correction was found to have a negligible impact on the 1D-Var retrievals. The use of low elevation angles improves the retrievals up to 12 % in RMSE in cloudy-sky in the first layers. The present implementation achieved a RMSE with respect to radiosondes within 1 K in clear-sky and 1.3 K in cloudy-sky conditions for temperature.

  11. Development, Capabilities, and Impact on Wind Analyses of the Hurricane Imaging Radiometer (HIRAD)

    Science.gov (United States)

    Miller, T.; Amarin, R.; Atlas, R.; Bailey, M.; Black, P.; Buckley, C.; Chen, S.; El-Nimri, S.; Hood, R.; James, M.; hide

    2010-01-01

    The Hurricane Imaging Radiometer (HIRAD) is a new airborne microwave remote sensor for hurricane observations that is currently under development by NASA Marshall Space Flight Center in partnership with the NOAA Atlantic Oceanographic and Meteorological Laboratory/Hurricane Research Division, the University of Central Florida, the University of Michigan, and the University of Alabama in Huntsville. The instrument is being test flown in January and is expected to participate in the tropical cyclone experiment GRIP (Genesis and Rapid Intensification Processes) in the 2010 season. HIRAD is being designed to study the wind field in some detail within strong hurricanes and to enhance the real-time airborne ocean surface winds observation capabilities of NOAA and USAF Weather Squadron hurricane hunter aircraft currently using the operational Stepped Frequency Microwave Radiometer (SFMR). Unlike SFMR, which measures wind speed and rain rate along the ground track at a single point directly beneath the aircraft, HIRAD will provide images of the surface wind and rain field over a wide swath (approximately 3 x the aircraft altitude) with approximately 2 km resolution. This paper describes the HIRAD instrument and the physical basis for its operations, including chamber test data from the instrument. The potential value of future HIRAD observations will be illustrated with a summary of Observing System Simulation Experiments (OSSEs) in which measurements from the new instrument as well as those from existing instruments (air, surface, and space-based) are simulated from the output of a detailed numerical model, and those results are used to construct simulated H*Wind analyses. Evaluations will be presented on the impact on H*Wind analyses of using the HIRAD instrument observations to replace those of the SFMR instrument, and also on the impact of a future satellite-based HIRAD in comparison to instruments with more limited capabilities for observing strong winds through heavy

  12. Preclinical evaluation of somatostatin analogs bearing two macrocyclic chelators for high specific activity labeling with radiometals

    International Nuclear Information System (INIS)

    Storch, D.; Schmitt, J.S.; Waldherr, C.; Maecke, H.R.; Waser, B.; Reubi, J.C.

    2007-01-01

    Radiometallated analogues of the regulatory peptide somatostatin are of interest in the in vivo localization and targeted radiotherapy of somatostatin receptor-overexpressing tumors. An important aspect of their use in vivo is a fast and efficient labeling (complexation) protocol for radiometals along with a high specific activity. We describe in this manuscript synthetic methods for the coupling of two chelators (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid = DOTA) to the bioactive peptide [Tyr 3 ,Thr 8 ]-octreotide (TATE) in order to increase the specific activity (radioactivity in Bq per mole peptide). The full chelator-linker-peptide conjugate was assembled on solid support using standard Fmoc chemistry. Two DOTA-chelators were linked to the peptide using lysine or N,N'-bis(3-aminopropyl)-glycine (Apg); in addition, pentasarcosine (Sar 5 ) was used as a spacer between the chelators and the peptide to probe its influence on biology and pharmacology. Complexation rates with In 3+ and Y 3+ salts and the corresponding radiometals were high, the bis-DOTA-derivatives showed higher complexation rates and gave higher specific activity than DOTA-TATE. Pharmacological and biological data of the complexed molecules did not show significant differences if compared to the parent peptide [ 111/nat In-DOTA]-TATE except for [( 111/nat In-DOTA) 2 -Apg]-TATE which showed a lower binding affinity and rate of internalization into tumor cells. The biodistribution of [( 111/nat In-DOTA)-Lys( 111/nat In-DOTA)]-TATE in the rat tumor model (AR4-2J) showed a high and specific (as shown by a blocking experiment) tracer uptake in somatostatin receptor-positive tissue but a lower tumor uptake compared to [ 111/nat In-DOTA]-TATE. (orig.)

  13. Use of ground-based radiometers for L-Band Freeze/Thaw retrieval in a boreal forest site

    Science.gov (United States)

    Roy, A.; Sonnentag, O.; Derksen, C.; Toose, P.; Pappas, C.; Mavrovic, A.; El Amine, M.; Royer, A.; Berg, A. A.; Rowlandson, T. L.; Barr, A.; Black, T. A.

    2017-12-01

    The boreal forest is the second largest land biome in the world and thus plays a major role in the global and regional climate systems. The extent, timing and duration of the seasonal freeze/thaw (F/T) state influences vegetation developmental stages (phenology) and, consequently, constitutes an important control on how boreal forest ecosystems exchange carbon, water and energy with the atmosphere. Recently, new L-Band satellite-derived F/T information has become available. However, disentangling the seasonally differing contributions from forest overstory and understory vegetation, and the ground surface to the satellite signal remains challenging. Here we present results from an ongoing campaign with two L-Band surface-based radiometers (SBR) installed on a micrometeorological tower at the Southern Old Black Spruce site (53.99°N / 105.12°W) in central Saskatchewan. One radiometer unit is installed on top of the tower viewing the multi-layer vegetation canopy from above. A second radiometer unit is installed within the multi-layer canopy, viewing the understory and the ground surface only. The objectives of our study are to (i) disentangle the L-Band F/T signal contribution of boreal forest overstory from the combined understory and ground surface contribution, and (ii) link the L-Band F/T signal to related boreal forest structural and functional characteristics. Analysis of these radiometer measurements made from September to November 2016 shows that when the ground surface is thawed, the main contributor to both radiometer signals is soil moisture. The Pearson correlation coefficient between brightness temperature (TB) at vertical polarization (V-pol) and soil permittivity is 0.79 for the radiometer above the canopy and 0.74 for the radiometer below the canopy. Under cold conditions when the soil was thawed (snow insulation) and the trees were frozen (below 0°C), TB at V-pol is negatively correlated with tree permittivity. The freezing tree contribution to

  14. Stable configurations in social networks

    Science.gov (United States)

    Bronski, Jared C.; DeVille, Lee; Ferguson, Timothy; Livesay, Michael

    2018-06-01

    We present and analyze a model of opinion formation on an arbitrary network whose dynamics comes from a global energy function. We study the global and local minimizers of this energy, which we call stable opinion configurations, and describe the global minimizers under certain assumptions on the friendship graph. We show a surprising result that the number of stable configurations is not necessarily monotone in the strength of connection in the social network, i.e. the model sometimes supports more stable configurations when the interpersonal connections are made stronger.

  15. Development of Stable Isotope Technology

    International Nuclear Information System (INIS)

    Jeong, Do Young; Kim, Cheol Jung; Han, Jae Min

    2009-03-01

    KAERI has obtained an advanced technology with singular originality for laser stable isotope separation. Objectives for this project are to get production technology of Tl-203 stable isotope used for medical application and are to establish the foundation of the pilot system, while we are taking aim at 'Laser Isotope Separation Technology to make resistance to the nuclear proliferation'. And we will contribute to ensuring a nuclear transparency in the world society by taking part in a practical group of NSG and being collaboration with various international groups related to stable isotope separation technology

  16. Red Blood Cell Antibody Identification

    Science.gov (United States)

    ... antibodies may or may not be associated with adverse reactions, and identification of the specific type of RBC ... the only things that can cause a transfusion reaction. The recipient's immune ... or to drugs that the donor may have taken. Rarely, antibodies in the plasma ...

  17. Library of monoclonal antibodies against brush border membrane epithelial antigens

    International Nuclear Information System (INIS)

    Behar, M.; Katz, A.; Silverman, M.

    1986-01-01

    A purified fraction of proximal tubule brush border membranes (BBM) was prepared from dog kidney and used to immunize mice. The standard technique of hybridoma production was followed as described by Kohler and Milstein. Production of antibodies was detected by indirect immunofluorescence on dog kidney slices and by immunodot against the purified fraction on nitrocellulose. Five hybrids exhibited anti BBM activity. These were cloned twice and yielded stable cell lines producing IgG type monoclonal antibodies against BBM. They were designated A 1 , C 7 , D 3 , D 7 and H 4 . As a family these five monoclonals have broad tissue specificity, i.e. positive staining of the surface mucosa of intestinal kidney proximal tubules. D 3 exhibits even broader specificity for epithelium reacting with bile canaliculi and choroid plexus. The authors have verified that at least 4/5 antibodies are directed against BBM protein as revealed by immunoprecipitation of solubilized BBM and detected by Coomassie blue staining or autoradiography of lactoperoxidase labelled BBM. Most interestingly all antibodies bind to the surface of LL CPK 1 cells, a continuous pig kidney cell line of undefined origin but exhibiting many characteristics of proximal tubule cells. The library of monoclonal antibodies obtained provide important probes with which to study membrane biogenesis and polarization in epithelial cells

  18. Structural Characterization of Peptide Antibodies

    DEFF Research Database (Denmark)

    Chailyan, Anna; Marcatili, Paolo

    2015-01-01

    The role of proteins as very effective immunogens for the generation of antibodies is indisputable. Nevertheless, cases in which protein usage for antibody production is not feasible or convenient compelled the creation of a powerful alternative consisting of synthetic peptides. Synthetic peptides...... can be modified to obtain desired properties or conformation, tagged for purification, isotopically labeled for protein quantitation or conjugated to immunogens for antibody production. The antibodies that bind to these peptides represent an invaluable tool for biological research and discovery....... To better understand the underlying mechanisms of antibody-antigen interaction here we present a pipeline developed by us to structurally classify immunoglobulin antigen binding sites and to infer key sequence residues and other variables that have a prominent role in each structural class....

  19. Application of ground-based, multi-channel microwave radiometer in the nowcasting of intense convective weather through instability indices of the atmosphere

    Energy Technology Data Exchange (ETDEWEB)

    Chan, P.W.; Hon, K.K. [Hong Kong Observatory, Hong Kong (China)

    2011-08-15

    A ground-based microwave radiometer gives the possibility of providing continuously available temperature and humidity profiles of the troposphere, from which instability indices of the atmosphere could be derived. This paper studies the possibility of correlating the radiometer-based instability indices with the occurrence of intense convective activity, namely, the occurrence of lightning. The correlation so established could be useful for the nowcasting of convective weather: the weather forecaster follows the evolution of the radiometer-based instability indices in order to access the chance for lightning to occur. The quality of the radiometer-based instability indices is first established by comparing with the radiosonde-based indices. Though there are biases and spreads in the scatter plots of the two datasets, the radiometer-based indices appear to follow the trend of the radiosonde-based indices in spite of the differences in measurement locations and working principles of the two instruments. The thresholds of instability indices for the occurrence of lightning (using 1 discharge) are then determined, specifically for the radiometer in use and the climatological condition in Hong Kong. It turns out that, among all the indices considered in this paper, KI has the best performance in terms of probability of detection of lightning occurrence, particularly for non-summer months, by using an optimum threshold. Finally, the correlation between the instability index and the amount of lightning strokes (within a certain distance from the radiometer) is established. It turns out that the correlation is the best using the minimum value of humidity index, with correlation coefficient of 0.55. The distance from the radiometer considered is about 30 km (having the best correlation between the number of lightning discharges and the instability index), which may be taken as the area over which the radiometer's measurement is considered to be representative of the

  20. Radiolabeled antibodies in cancer. Oncology Overview

    International Nuclear Information System (INIS)

    1984-11-01

    Oncology Overviews are a service of the International Cancer Research Data Bank (ICRDB) Program of the National Cancer Institute, intended to facilitate and promote the exchange of information between cancer scientists by keeping them aware of literature related to their research being published by other laboratories through the world. Each Oncology Overview represents a survey of the literature associated with a selected area of cancer research. It contains abstracts of articles which have been selected and organized by researchers associated with the field. Contents: Radiolabeled antibodies--labeling and imaging techniques; Radiolabeled antibodies--carcinoembryonic antigen; Radiolabeled antibodies--alpha-fetoprotein; Radiolabeled antibodies--human chorionic gonadotropin; Radiolabeled antibodies--ferritin; Radiolabeled antibodies--imaging of colorectal tumors; Radiolabeled antibodies--imaging of malignant melanoma; Radiolabeled antibodies--imaging of urogenital tumors; Radiolabeled antibodies--imaging of thyroid tumors; Radiolabeled antibodies--other clinical studies; Radiolabeled antibodies--selected preclinical studies; Radiolabeled antibodies--reviews

  1. New perspectives on recombinant human antibodies

    NARCIS (Netherlands)

    J. de Kruif (John); A.-R. van der Vuurst de Vries (Anne); L. Cilenti (L.); E. Boel (E.); W. van Ewijk (Willem); T. Logtenberg (Ton)

    1996-01-01

    textabstractThe limited potential of murine monoclonal antibodies for human immunotherapy has driven recent progress in recombinant antibody technology. Here, de Kruif and colleagues report on advances in the development and use of phage-antibody-display libraries.

  2. Measurement of antibodies to tubulin by radioimmunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Mead, G M; Cowin, P; Whitehouse, J M.A. [CRC Medical Oncology Unit, Southampton General Hospital, Southampton, U.K.

    1979-07-24

    A solid-phase double antibody radioimmunoassay capable of measuring antibody to tubulin, the principal component of microtubules, is described. This assay is simple, combining sensitivity with specificity and also allowing determination of antibody subclasses.

  3. Stable isotope research pool inventory

    International Nuclear Information System (INIS)

    1984-03-01

    This report contains a listing of electromagnetically separated stable isotopes which are available at the Oak Ridge National Laboratory for distribution for nondestructive research use on a loan basis. This inventory includes all samples of stable isotopes in the Research Materials Collection and does not designate whether a sample is out on loan or is in reprocessing. For some of the high abundance naturally occurring isotopes, larger amounts can be made available; for example, Ca-40 and Fe-56

  4. French days on stable isotopes

    International Nuclear Information System (INIS)

    2000-01-01

    These first French days on stable isotopes took place in parallel with the 1. French days of environmental chemistry. Both conferences had common plenary sessions. The conference covers all aspects of the use of stable isotopes in the following domains: medicine, biology, environment, tracer techniques, agronomy, food industry, geology, petroleum geochemistry, cosmo-geochemistry, archaeology, bio-geochemistry, hydrology, climatology, nuclear and particle physics, astrophysics, isotope separations etc.. Abstracts available on CD-Rom only. (J.S.)

  5. Stable isotope research pool inventory

    International Nuclear Information System (INIS)

    1982-01-01

    This report contains a listing of electromagnetically separated stable isotopes which are available for distribution within the United States for nondestructive research use from the Oak Ridge National Laboratory on a loan basis. This inventory includes all samples of stable isotopes in the Material Research Collection and does not designate whether a sample is out on loan or in reprocessing. For some of the high abundance naturally occurring isotopes, larger amounts can be made available; for example, Ca-40 and Fe-56

  6. Pharmaceuticals labelled with stable isotopes

    International Nuclear Information System (INIS)

    Krumbiegel, P.

    1986-11-01

    The relatively new field of pharmaceuticals labelled with stable isotopes is reviewed. Scientific, juridical, and ethical questions are discussed concerning the application of these pharmaceuticals in human medicine. 13 C, 15 N, and 2 H are the stable isotopes mainly utilized in metabolic function tests. Methodical contributions are given to the application of 2 H, 13 C, and 15 N pharmaceuticals showing new aspects and different states of development in the field under discussion. (author)

  7. An optimized antibody-chelator conjugate for imaging of carcinoembryonic antigen with indium-111

    International Nuclear Information System (INIS)

    Sumerdon, G.A.; Rogers, P.E.; Lombardo, C.M.; Schnobrich, K.E.; Melvin, S.L.; Tribby, I.I.E.; Stroupe, S.D.; Johnson, D.K.; Hobart, E.D.

    1990-01-01

    A monoclonal antibody to carcinoembryonic antigen showing minimal cross-reactivity with blood cells and normal tissues was derivatized with benzylisothiocyanate derivatives of EDTA and DTPA. Seven chelators per immunoglobulin could be incorporated without loss of immunoreactivity. The resulting conjugates, labeled with indium-111, showed low liver uptake in animals. A cold kit, comprising the DTPA conjugate at a molarity of antibody bound chelator exceeding 1 x 10 -4 M, gave radiochemical yields of indium labeled antibody of ≥ 95% and was stable for 1 yr. (author)

  8. Mapping stable direct and retrograde orbits around the triple system of asteroids (45) Eugenia

    Science.gov (United States)

    Araujo, R. A. N.; Moraes, R. V.; Prado, A. F. B. A.; Winter, O. C.

    2017-12-01

    It is widely accepted that knowing the composition and the orbital evolution of asteroids might help us to understand the process of formation of the Solar system. It is also known that asteroids can represent a threat to our planet. Such an important role has made space missions to asteroids a very popular topic in current astrodynamics and astronomy studies. Taking into account the increasing interest in space missions to asteroids, especially to multiple systems, we present a study that aims to characterize the stable and unstable regions around the triple system of asteroids (45) Eugenia. The goal is to characterize the unstable and stable regions of this system and to make a comparison with the system 2001 SN263, which is the target of the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) mission. A new concept was used for mapping orbits, by considering the disturbance received by the spacecraft from all perturbing forces individually. This method has also been applied to (45) Eugenia. We present the stable and unstable regions for particles with relative inclination between 0° and 180°. We found that (45) Eugenia presents larger stable regions for both prograde and retrograde cases. This is mainly because the satellites of this system are small when compared to the primary body, and because they are not close to each other. We also present a comparison between these two triple systems, and we discuss how these results can guide us in the planning of future missions.

  9. Sea Surface Temperature Records from the Along-Track Scanning Radiometers

    Science.gov (United States)

    Mutlow, C. T.; Smith, D. L.; Delderfield, J.; Llewellyn-Jones, D.

    2006-12-01

    Since the early 1990s ESA has flown Along Track Scanning Radiometer (ATSR) instruments on its ERS-1 and -2 satellites and is currently flying the Advanced ATSR instrument (AATSR) on its very successful Envisat mission; each successive sensor has been an incremental improvement over the last. The sensors have been specifically designed to provide the information urgently needed for the debate on climate change and global warming, as well as to produce properly calibrated image data sets for use in a wide range of EO studies. The ATSR instruments are a series of second generation space radiometers which build on the long heritage of the NOAA Advanced Very High Resolution Radiometers (AVHRR). Each ATSR exploits the multi-channel method pioneered in AVHRR but also uses new technology to improve instrument stability and calibration, detector signal to noise, and to provide observations of the same surface scene at two different angles. The novel feature of each ATSR, from which the sensor is derives its name, is its use of along-track scanning to reduce the effects of the atmosphere on surface measurements. This method obtains two observations of the scene through differing amounts of atmosphere; the "along track" view passes through a longer atmospheric path so is more affected by the atmosphere than the nadir view. ATSR-1 was launched on the ESA ERS-1 satellite on 17th July 1991, as the test-bed for the along track scanning concept using infrared channels at 1.6, 3.7, 10.8 and 12.0um. ATSR-1 continued to operate until the ERS-1 spacecraft was lost some 10 years after launch. The ATSR-2 and Advanced ATSR (AATSR) instruments are developments from the original ATSR-1, which in addition to the infrared channels; carry extra visible channels at 0.55, 0.67 and 0.87um for vegetation, cloud and aerosol remote sensing. ATSR-2 has operating on the ESA ERS-2 satellite since April 1995 and has provided over 10 years of data. The current operational sensor is AATSR flying on ESA

  10. An alternative method for calibration of narrow band radiometer using a radiative transfer model

    Energy Technology Data Exchange (ETDEWEB)

    Salvador, J; Wolfram, E; D' Elia, R [Centro de Investigaciones en Laseres y Aplicaciones, CEILAP (CITEFA-CONICET), Juan B. de La Salle 4397 (B1603ALO), Villa Martelli, Buenos Aires (Argentina); Zamorano, F; Casiccia, C [Laboratorio de Ozono y Radiacion UV, Universidad de Magallanes, Punta Arenas (Chile) (Chile); Rosales, A [Universidad Nacional de la Patagonia San Juan Bosco, UNPSJB, Facultad de Ingenieria, Trelew (Argentina) (Argentina); Quel, E, E-mail: jsalvador@citefa.gov.ar [Universidad Nacional de la Patagonia Austral, Unidad Academica Rio Gallegos Avda. Lisandro de la Torre 1070 ciudad de Rio Gallegos-Sta Cruz (Argentina) (Argentina)

    2011-01-01

    The continual monitoring of solar UV radiation is one of the major objectives proposed by many atmosphere research groups. The purpose of this task is to determine the status and degree of progress over time of the anthropogenic composition perturbation of the atmosphere. Such changes affect the intensity of the UV solar radiation transmitted through the atmosphere that then interacts with living organisms and all materials, causing serious consequences in terms of human health and durability of materials that interact with this radiation. One of the many challenges that need to be faced to perform these measurements correctly is the maintenance of periodic calibrations of these instruments. Otherwise, damage caused by the UV radiation received will render any one calibration useless after the passage of some time. This requirement makes the usage of these instruments unattractive, and the lack of frequent calibration may lead to the loss of large amounts of acquired data. Motivated by this need to maintain calibration or, at least, know the degree of stability of instrumental behavior, we have developed a calibration methodology that uses the potential of radiative transfer models to model solar radiation with 5% accuracy or better relative to actual conditions. Voltage values in each radiometer channel involved in the calibration process are carefully selected from clear sky data. Thus, tables are constructed with voltage values corresponding to various atmospheric conditions for a given solar zenith angle. Then we model with a radiative transfer model using the same conditions as for the measurements to assemble sets of values for each zenith angle. The ratio of each group (measured and modeled) allows us to calculate the calibration coefficient value as a function of zenith angle as well as the cosine response presented by the radiometer. The calibration results obtained by this method were compared with those obtained with a Brewer MKIII SN 80 located in the

  11. Assessment of Satellite-Derived Surface Reflectances by NASA's CAR Airborne Radiometer over Railroad Valley, Nevada

    Science.gov (United States)

    Kharbouche, Said; Muller, Jan-Peter; Gatebe, Charles K.; Scanlon, Tracy; Banks, Andrew C.

    2017-01-01

    CAR (Cloud Absorption Radiometer) is a multi-angular and multi-spectral airborne radiometer instrument, whose radiometric and geometric characteristics are well calibrated and adjusted before and after each flight campaign. CAR was built by NASA (National Aeronautics and Space Administration) in 1984. On 16 May 2008, a CAR flight campaign took place over the well-known calibration and validation site of Railroad Valley in Nevada (38.504 deg N, 115.692 deg W).The campaign coincided with the overpasses of several key EO (Earth Observation) satellites such as Landsat-7, Envisat and Terra. Thus, there are nearly simultaneous measurements from these satellites and the CAR airborne sensor over the same calibration site. The CAR spectral bands are close to those of most EO satellites. CAR has the ability to cover the whole range of azimuth view angles and a variety of zenith angles depending on altitude and, as a consequence, the biases seen between satellite and CAR measurements due to both unmatched spectral bands and unmatched angles can be significantly reduced. A comparison is presented here between CARs land surface reflectance (BRF or Bidirectional Reflectance Factor) with those derived from Terra/MODIS (MOD09 and MAIAC), Terra/MISR, Envisat/MERIS and Landsat-7. In this study, we utilized CAR data from low altitude flights (approx. 180 m above the surface) in order to minimize the effects of the atmosphere on these measurements and then obtain a valuable ground-truth data set of surface reflectance. Furthermore, this study shows that differences between measurements caused by surface heterogeneity can be tolerated, thanks to the high homogeneity of the study site on the one hand, and on the other hand, to the spatial sampling and the large number of CAR samples. These results demonstrate that satellite BRF measurements over this site are in good agreement with CAR with variable biases across different spectral bands. This is most likely due to residual aerosol

  12. Enhanced tumor retention of a radiohalogen label for site-specific modification of antibodies.

    Science.gov (United States)

    Boswell, C Andrew; Marik, Jan; Elowson, Michael J; Reyes, Noe A; Ulufatu, Sheila; Bumbaca, Daniela; Yip, Victor; Mundo, Eduardo E; Majidy, Nicholas; Van Hoy, Marjie; Goriparthi, Saritha N; Trias, Anthony; Gill, Herman S; Williams, Simon P; Junutula, Jagath R; Fielder, Paul J; Khawli, Leslie A

    2013-12-12

    A known limitation of iodine radionuclides for labeling and biological tracking of receptor targeted proteins is the tendency of iodotyrosine to rapidly diffuse from cells following endocytosis and lysosomal degradation. In contrast, radiometal-chelate complexes such as indium-111-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (In-111-DOTA) accumulate within target cells due to the residualizing properties of the polar, charged metal-chelate-amino acid adduct. Iodine radionuclides boast a diversity of nuclear properties and chemical means for incorporation, prompting efforts to covalently link radioiodine with residualizing molecules. Herein, we describe the Ugi-assisted synthesis of [I-125]HIP-DOTA, a 4-hydroxy-3-iodophenyl (HIP) derivative of DOTA, and demonstration of its residualizing properties in a murine xenograft model. Overall, this study displays the power of multicomponent synthesis to yield a versatile radioactive probe for antibodies across multiple therapeutic areas with potential applications in both preclinical biodistribution studies and clinical radioimmunotherapies.

  13. Design and First Results of an UAV-Borne L-Band Radiometer for Multiple Monitoring Purposes

    Directory of Open Access Journals (Sweden)

    Rene Acevo-Herrera

    2010-06-01

    Full Text Available UAV (unmanned Aerial Vehicle platforms represent a challenging opportunity for the deployment of a number of remote sensors. These vehicles are a cost-effective option in front of manned aerial vehicles (planes and helicopters, are easy to deploy due to the short runways needed, and they allow users to meet the critical requirements of the spatial and temporal resolutions imposed by the instruments. L-band radiometers are an interesting option for obtaining soil moisture maps over local areas with relatively high spatial resolution for precision agriculture, coastal monitoring, estimation of the risk of fires, flood prevention, etc. This paper presents the design of a light-weight, airborne L-band radiometer for deployment in a small UAV, including the hardware and specific software developed for calibration, geo-referencing, and soil moisture retrieval. First results and soil moisture retrievals from different field experiments are presented.

  14. Application of microwave radiometer and wind profiler data in the estimation of wind gust associated with intense convective weather

    International Nuclear Information System (INIS)

    Chan, P W; Wong, K H

    2008-01-01

    Estimates of the wind gusts associated with intense convective weather could be obtained using empirical relationships such as GUSTEX based on radiosonde measurements. However, such data are only available a couple of times a day and may not reflect the rapidly changing atmospheric condition in spring and summer times. The feasibility of combining the thermodynamic profiles from a ground-based microwave radiometer and wind profiles given by radar wind profilers in the continuous estimation of wind gusts is studied in this paper. Based on the results of a 4-month trial of a microwave radiometer in Hong Kong in 2004, the estimated and the actual gusts are reasonably well correlated. It is also found that the wind gusts so estimated provide better indications of the strength of squalls compared with those based on radiosonde measurements and with a lead time of about one hour

  15. GHRSST Level 2P Global Sea Surface Temperature from the Visible Infrared Imaging Radiometer Suite (VIIRS) on the Suomi NPP satellite (GDS version 2)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Visible and Infrared Imager/Radiometer Suite (VIIRS) is a multi-disciplinary instrument that is being flown on the Joint Polar Satellite System (JPSS) series of...

  16. GHRSST Level 3P North Atlantic Regional Subskin Sea Surface Temperature from the Advanced Very High Resolution Radiometer (AVHRR) on NOAA-19 (GDS version 1)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A Group for HIgh Resolution Sea Surface Temperature (GHRSST) dataset for the North Atlantic Region (NAR) from the Advanced Very High Resolution Radiometer (AVHRR) on...

  17. GHRSST Level 3P Global Subskin Sea Surface Temperature from the Advanced Very High Resolution Radiometer (AVHRR) on the MetOp-A satellite (GDS version 1)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A global Level 3 Group for HIgh Resolution Sea Surface Temperature (GHRSST) dataset from the Advanced Very High Resolution Radiometer (AVHRR) on the MetOp-A platform...

  18. GHRSST Level 2P North Atlantic Regional Bulk Sea Surface Temperature from the Advanced Very High Resolution Radiometer (AVHRR) on the NOAA-18 satellite (GDS version 1)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A Group for HIgh Resolution Sea Surface Temperature (GHRSST) dataset for the North Atlantic Region (NAR) from the Advanced Very High Resolution Radiometer (AVHRR) on...

  19. GHRSST Level 2P Gridded Global Subskin Sea Surface Temperature from WindSat polarimetric radiometer on the Coriolis satellite (GDS version 1)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains sea surface temperature derived from observations made by the WindSat Polarimetric Radiometer developed by the Naval Research Laboratory (NRL)...

  20. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Aerosol Optical Thickness (AOT) and Aerosol Particle Size Parameter (APSP) Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Aerosol Optical Thickness (AOT) from the Visible Infrared Imaging Radiometer...

  1. GHRSST Level 3U Global Subskin Sea Surface Temperature from the WindSat Polarimetric Radiometer on the Coriolis satellite (GDS version 2)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The WindSat Polarimetric Radiometer, launched on January 6, 2003 aboard the Department of Defense Coriolis satellite, was designed to measure the ocean surface wind...

  2. GHRSST Level 2P Global Subskin Sea Surface Temperature from the Advanced Microwave Scanning Radiometer 2 on the GCOM-W satellite (GDS version 2)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Advanced Microwave Scanning Radiometer 2 (AMSR2) was launched on 18 May 2012, onboard the Global Change Observation Mission - Water (GCOM-W) satellite developed...

  3. GHRSST Level 3C North Atlantic Regional Subskin Sea Surface Temperature from the Advanced Very High Resolution Radiometer (AVHRR) on NOAA-19 (GDS version 2)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A Group for High Resolution Sea Surface Temperature (GHRSST) dataset for the North Atlantic Region (NAR) from the Advanced Very High Resolution Radiometer (AVHRR) on...

  4. SMAP/Sentinel-1 L2 Radiometer/Radar 30-Second Scene 3 km EASE-Grid Soil Moisture V001

    Data.gov (United States)

    National Aeronautics and Space Administration — This Level-2 (L2) soil moisture product provides estimates of land surface conditions retrieved by both the Soil Moisture Active Passive (SMAP) radiometer during...

  5. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Snow Cover/Depth (SCD) Snow Fraction Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Snow Cover/Depth Fraction (SCF) from the Visible Infrared Imaging Radiometer...

  6. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Snow Cover/Depth (SCD) Binary Map Environmental Data Record (EDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of Binary Snow Cover (BSC) from the Visible Infrared Imaging Radiometer Suite...

  7. GHRSST Level 2P North Atlantic Regional Bulk Sea Surface Temperature from the Advanced Very High Resolution Radiometer (AVHRR) on the NOAA-17 satellite (GDS version 1)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A Group for High Resolution Sea Surface Temperature (GHRSST) dataset for the North Atlantic Region (NAR) from the Advanced Very High Resolution Radiometer (AVHRR) on...

  8. GHRSST Level 2P North Atlantic Regional Bulk Sea Surface Temperature from the Advanced Very High Resolution Radiometer (AVHRR) on the NOAA-16 satellite (GDS version 1)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A Group for HIgh Resolution Sea Surface Temperature (GHRSST) dataset for the North Atlantic Region (NAR) from the Advanced Very High Resolution Radiometer (AVHRR) on...

  9. GHRSST Level 2P 1 m Depth Global Sea Surface Temperature from the Visible Infrared Imaging Radiometer Suite (VIIRS) on the Suomi NPP satellite (GDS version 2)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A global Group for High Resolution Sea Surface Temperature (GHRSST) Level 2P dataset based on retrievals from the Visible Infrared Imaging Radiometer Suite (VIIRS)....

  10. An Airborne Campaign Measuring Wind Signatures from the Sea Surface using an L-band Polarimetric Radiometer

    DEFF Research Database (Denmark)

    Søbjærg, Sten Schmidl; Skou, Niels

    2003-01-01

    A series of circle flights have been carried out over the sea surface, using the EMIRAD L-band polarimetric radiometer. Motion compensation is applied, and polarimetric azimuth signatures are generated. Single tracks show geophysical noise, typically about 2 K, but averaging decreases the noise, ......, but a comparison of the signature to the downwelling galactic background radiation indicates, that the signature may not origin from the wind driven sea surface pattern....

  11. Dissecting Immunogenicity of Monoclonal Antibodies

    National Research Council Canada - National Science Library

    Snyder, Christopher

    2003-01-01

    The potential of monoclonal antibodies, (mAbs), for use in therapeutic and diagnostic applications has not been fully realized in part due to counter-immune responses that often arise in patient recipients of mAb...

  12. A simple algorithm for identifying periods of snow accumulation on a radiometer

    Science.gov (United States)

    Lapo, Karl E.; Hinkelman, Laura M.; Landry, Christopher C.; Massmann, Adam K.; Lundquist, Jessica D.

    2015-09-01

    Downwelling solar, Qsi, and longwave, Qli, irradiances at the earth's surface are the primary energy inputs for many hydrologic processes, and uncertainties in measurements of these two terms confound evaluations of estimated irradiances and negatively impact hydrologic modeling. Observations of Qsi and Qli in cold environments are subject to conditions that create additional uncertainties not encountered in other climates, specifically the accumulation of snow on uplooking radiometers. To address this issue, we present an automated method for estimating these periods of snow accumulation. Our method is based on forest interception of snow and uses common meteorological observations. In this algorithm, snow accumulation must exceed a threshold to obscure the sensor and is only removed through scouring by wind or melting. The algorithm is evaluated at two sites representing different mountain climates: (1) Snoqualmie Pass, Washington (maritime) and (2) the Senator Beck Basin Study Area, Colorado (continental). The algorithm agrees well with time-lapse camera observations at the Washington site and with multiple measurements at the Colorado site, with 70-80% of observed snow accumulation events correctly identified. We suggest using the method for quality controlling irradiance observations in snow-dominated climates where regular, daily maintenance is not possible.

  13. A Fast Visible-Infrared Imaging Radiometer Suite Simulator for Cloudy Atmopheres

    Science.gov (United States)

    Liu, Chao; Yang, Ping; Nasiri, Shaima L.; Platnick, Steven; Meyer, Kerry G.; Wang, Chen Xi; Ding, Shouguo

    2015-01-01

    A fast instrument simulator is developed to simulate the observations made in cloudy atmospheres by the Visible Infrared Imaging Radiometer Suite (VIIRS). The correlated k-distribution (CKD) technique is used to compute the transmissivity of absorbing atmospheric gases. The bulk scattering properties of ice clouds used in this study are based on the ice model used for the MODIS Collection 6 ice cloud products. Two fast radiative transfer models based on pre-computed ice cloud look-up-tables are used for the VIIRS solar and infrared channels. The accuracy and efficiency of the fast simulator are quantify in comparison with a combination of the rigorous line-by-line (LBLRTM) and discrete ordinate radiative transfer (DISORT) models. Relative errors are less than 2 for simulated TOA reflectances for the solar channels and the brightness temperature differences for the infrared channels are less than 0.2 K. The simulator is over three orders of magnitude faster than the benchmark LBLRTM+DISORT model. Furthermore, the cloudy atmosphere reflectances and brightness temperatures from the fast VIIRS simulator compare favorably with those from VIIRS observations.

  14. Advances in real-time millimeter-wave imaging radiometers for avionic synthetic vision

    Science.gov (United States)

    Lovberg, John A.; Chou, Ri-Chee; Martin, Christopher A.; Galliano, Joseph A., Jr.

    1995-06-01

    Millimeter-wave imaging has advantages over conventional visible or infrared imaging for many applications because millimeter-wave signals can travel through fog, snow, dust, and clouds with much less attenuation than infrared or visible light waves. Additionally, passive imaging systems avoid many problems associated with active radar imaging systems, such as radar clutter, glint, and multi-path return. ThermoTrex Corporation previously reported on its development of a passive imaging radiometer that uses an array of frequency-scanned antennas coupled to a multichannel acousto-optic spectrum analyzer (Bragg-cell) to form visible images of a scene through the acquisition of thermal blackbody radiation in the millimeter-wave spectrum. The output from the Bragg cell is imaged by a standard video camera and passed to a computer for normalization and display at real-time frame rates. An application of this system is its incorporation as part of an enhanced vision system to provide pilots with a synthetic view of a runway in fog and during other adverse weather conditions. Ongoing improvements to a 94 GHz imaging system and examples of recent images taken with this system will be presented. Additionally, the development of dielectric antennas and an electro- optic-based processor for improved system performance, and the development of an `ultra- compact' 220 GHz imaging system will be discussed.

  15. The High Altitude MMIC Sounding Radiometer on the GLOBAL HAWK: From Technology Development to Science Discovery

    Science.gov (United States)

    Brown, Shannon; Denning, Richard; Lambrigtsen, Bjorn; Lim, Boon; Tanabe, Jordan; Tanner, Alan

    2013-01-01

    This paper presents results from the High Altitude MMIC Sounding Radiometer (HAMSR) during three recent field campaigns on the Global Hawk Unmanned Ariel Vehicles (UAV), focusing on the enabling technology that led to unprecedented observations of significant weather phenomenon, such as thermodynamic evolution of the tropical cyclone core during rapid intensification and the high resolution three dimensional mapping of several atmospheric river events. HAMSR is a 25 channel cross-track scanning microwave sounder with channels near the 60 and 118 GHz oxygen lines and the 183 GHz water vapor line. HAMSR was originally designed and built at the Jet Propulsion Laboratory as a technology demonstrator in 1998. Subsequent to this, HAMSR participated in three NASA hurricane field campaigns, CAMEX-4, TCSP and NAMMA. Beginning in 2008, HAMSR was extensively upgraded to deploy on the NASA Global Hawk (GH) platform and serve as an asset to the NASA sub-orbital program. HAMSR has participated on the Global Hawk during the 2010 Genesis and Rapid Intensification (GRIP) campaign, the 2011 Winter Storms and Atmospheric Rivers (WISPAR) campaign and is currently participating in the NASA Ventures Hurricane and Severe Storm Sentinel (HS3) campaign (2011-2015).

  16. Preliminary feasibility analysis of a pressure modulator radiometer for remote sensing of tropospheric constituents

    Science.gov (United States)

    Orr, H. D., III; Rarig, P. L.

    1981-01-01

    A pressure modulator radiometer operated in a nadir viewing mode from the top of a midlatitude summer model of the atmosphere was theoretically studied for monitoring the mean volumetric mixing ratio of carbon monoxide in the troposphere. The mechanical characteristics of the instrument on the Nimbus 7 stratospheric and mesospheric sounder experiment are assumed and CO is assumed to be the only infrared active constituent. A line by line radiative transfer computer program is used to simulate the upwelling radiation reaching the top of the atmosphere. The performance of the instrument is examined as a function of the mean pressure in and the length of the instrument gas correlation cell. Instrument sensitivity is described in terms of signal to noise ratio for a 10 percent change in CO mixing ratio. Sensitivity to mixing ratio changes is also studied. It is concluded that tropospheric monitoring requires a pressure modulator drive having a larger swept volume and producing higher compression ratios at higher mean cell pressures than the Nimbus 7 design.

  17. UV dosimetry in Antarctica (Baia Terranova): analysis of data from polysulphone films and GUV 511 radiometer

    Science.gov (United States)

    Mariutti, Gianni F.; Bortolin, Emanuela; Polichetti, Alessandro; Anav, Andrea; Casale, Giuseppe R.; Di Menno, Massimo; Rafanelli, Claudio

    2003-11-01

    This paper shows the results of measurements carried out in November 2002 in the Italian Antarctic Base of Baia Terranova (74.07°S, 164.08°E) to test polysulphone film badges as possible UV personal dosimeters in such extreme environmental conditions. In the Italian Antarctic Base a multichannel radiometer GUV 511 (Biospherical Inc.) is routinely used by the Italian National Research Council (CNR) for UV irradiance at sea level. This instrument measures the intensity of the solar UV spectrum at four different wavelengths: 305, 320, 340, 380 nm, respectively. Data obtained from polysulphone badges exposed in the horizontal and the vertical configurations during diverse time lapses of the day, and from polysulphone badges worn by three volunteers of the base staff during several outdoors activities, have been compared with the irradiance data calculated from the measured values of GUV 511. A preliminary analysis of the whole data, also in the light of other recorded atmospheric and climatic parameters, shows a reasonable consistency. As also shown by previous measurements, carried out in June 2002 in the locality of Ny Alesund (Svalbard -- Artic Region), the calibration of the above mentioned personal dosimeters by means of another instrument operating in the same locality is a crucial step. Further work is required to demonstrate this approach is suitable for an acceptable evaluation of personal radiant exposures.

  18. Broadband Outdoor Radiometer Calibration Process for the Atmospheric Radiation Measurement Program

    Energy Technology Data Exchange (ETDEWEB)

    Dooraghi, Michael [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2015-09-01

    The Atmospheric Radiation Measurement program (ARM) maintains a fleet of monitoring stations to aid in the improved scientific understanding of the basic physics related to radiative feedback processes in the atmosphere, particularly the interactions among clouds and aerosols. ARM obtains continuous measurements and conducts field campaigns to provide data products that aid in the improvement and further development of climate models. All of the measurement campaigns include a suite of solar measurements. The Solar Radiation Research Laboratory at the National Renewable Energy Laboratory supports ARM's full suite of stations in a number of ways, including troubleshooting issues that arise as part of the data-quality reviews; managing engineering changes to the standard setup; and providing calibration services and assistance to the full fleet of solar-related instruments, including pyranometers, pyrgeometers, pyrheliometers, as well as the temperature/relative humidity probes, multimeters, and data acquisition systems that are used in the calibrations performed at the Southern Great Plains Radiometer Calibration Facility. This paper discusses all aspects related to the support provided to the calibration of the instruments in the solar monitoring fleet.

  19. Total solar irradiance as measured by the SOVAP radiometer onboard PICARD

    Directory of Open Access Journals (Sweden)

    Meftah Mustapha

    2016-01-01

    Full Text Available From the SOlar VAriability PICARD (SOVAP space-based radiometer, we obtained a new time series of the total solar irradiance (TSI during Solar Cycle 24. Based on SOVAP data, we obtained that the TSI input at the top of the Earth’s atmosphere at a distance of one astronomical unit from the Sun is 1361.8 ± 2.4 W m−2 (1σ representative of the 2008 solar minimum period. From 2010 to 2014, the amplitude of the changes has been of the order of ± 0.1%, corresponding to a range of about 2.7 W m−2. To determine the TSI from SOVAP, we present here an improved instrument equation. A parameter was integrated from a theoretical analysis that highlighted the thermo-electrical non-equivalence of the radiometric cavity. From this approach, we obtained values that are lower than those previously provided with the same type of instrument. The results in this paper supersede the previous SOVAP analysis and provide the best SOVAP-based TSI-value estimate and its temporal variation.

  20. Development of the Multi-Angle Stratospheric Aerosol Radiometer (MASTAR) Instrument

    Science.gov (United States)

    DeLand, M. T.; Colarco, P. R.; Kowalewski, M. G.; Gorkavyi, N.; Ramos-Izquierdo, L.

    2017-12-01

    Aerosol particles in the stratosphere ( 15-25 km altitude), both produced naturally and perturbed by volcanic eruptions and anthropogenic emissions, continue to be a source of significant uncertainty in the Earth's energy budget. Stratospheric aerosols can offset some of the warming effects caused by greenhouse gases. These aerosols are currently monitored using measurements from the Ozone Mapping and Profiling Suite (OMPS) Limb Profiler (LP) instrument on the Suomi NPP satellite. In order to improve the sensitivity and spatial coverage of these aerosol data, we are developing an aerosol-focused compact version of the OMPS LP sensor called Multi-Angle Stratospheric Aerosol Radiometer (MASTAR) to fly on a 3U Cubesat satellite, using a NASA Instrument Incubator Program (IIP) grant. This instrument will make limb viewing measurements of the atmosphere in multiple directions simultaneously, and uses only a few selected wavelengths to reduce size and cost. An initial prototype version has been constructed using NASA GSFC internal funding and tested in the laboratory. Current design work is targeted towards a preliminary field test in Spring 2018. We will discuss the scientific benefits of MASTAR and the status of the project.

  1. Real-time millimeter-wave imaging radiometer for avionic synthetic vision

    Science.gov (United States)

    Lovberg, John A.; Chou, Ri-Chee; Martin, Christopher A.

    1994-07-01

    ThermoTrex Corporation (TTC) has developed an imaging radiometer, the passive microwave camera (PMC), that uses an array of frequency-scanned antennas coupled to a multi-channel acousto-optic (Bragg cell) spectrum analyzer to form visible images of a scene through acquisition of thermal blackbody radiation in the millimeter-wave spectrum. The output of the Bragg cell is imaged by a standard video camera and passed to a computer for normalization and display at real-time frame rates. One application of this system could be its incorporation into an enhanced vision system to provide pilots with a clear view of the runway during fog and other adverse weather conditions. The unique PMC system architecture will allow compact large-aperture implementations because of its flat antenna sensor. Other potential applications include air traffic control, all-weather area surveillance, fire detection, and security. This paper describes the architecture of the TTC PMC and shows examples of images acquired with the system.

  2. Differential Radiometers Using Fabry-Perot Interferometric Technique for Remote Sensing of Greenhouse Gases

    Science.gov (United States)

    Georgieva, Elena M.; Heaps,William S.; Wilson, Emily L.

    2007-01-01

    A new type of remote sensing radiometer based upon the Fabry-Perot interferometric technique has been developed at NASA's Goddard Space Flight Center and tested from both ground and aircraft platform. The sensor uses direct or reflected sunlight and has channels for measuring column concentration of carbon dioxide at 1570 nm, oxygen lines sensitive to pressure and temperature at 762 and 768 nm, and water vapor (940 nm). A solid Fabry-Perot etalon is used as a tunable narrow bandpass filter to restrict the measurement to the gas of interest's absorption bands. By adjusting the temperature of the etalon, which changes the index of refraction of its material, the transmission fringes can be brought into nearly exact correspondence with absorption lines of the particular species. With this alignment between absorption lines and fringes, changes in the amount of a species in the atmosphere strongly affect the amount of light transmitted by the etalon and can be related to gas concentration. The technique is applicable to different chemical species. We have performed simulations and instrument design studies for CH4, "Cot isotope, and CO detection. Index Terms- Absorbing media, Atmospheric measurements, Fabry-Perot interferometers, Optical interferometry, Remote sensing.

  3. Geostationary Coastal and Air Pollution Events (GeoCAPE) Filter Radiometer (FR)

    Science.gov (United States)

    Kotecki, Carl; Chu, Martha; Wilson, Mark; Clark, Mike; Nanan, Bobby; Matson, Liz; McBirney, Dick; Smith, Jay; Earle, Paul; Choi, Mike; hide

    2014-01-01

    The GeoCAPE Filter Radiometer (FR) Study is a different instrument type than all of the previous IDL GeoCape studies. The customer primary goals are to keep mass, volume and cost to a minimum while meeting the science objectives and maximizing flight opportunities by fitting on the largest number of GEO accommodations possible. Minimize total mission costs by riding on a commercial GEO satellite. For this instrument type, the coverage rate, km 2 min, was significantly increased while reducing the nadir ground sample size to 250m. This was accomplished by analyzing a large 2d area for each integration period. The field of view will be imaged on a 4k x 4k detector array of 15 micrometer pixels. Each ground pixel is spread over 2 x 2 detector pixels so the instantaneous field of view (IFOV) is 2048 X 2048 ground pixels. The baseline is, for each field of view 50 sequential snapshot images are taken, each with a different filter, before indexing the scan mirror to the next IFOV. A delta would be to add additional filters.

  4. Atmospheric water distribution in a midlatitude cyclone observed by the Seasat Scanning Multichannel Microwave Radiometer

    Science.gov (United States)

    Mcmurdie, L. A.; Katsaros, K. B.

    1985-01-01

    Patterns in the horizontal distribution of integrated water vapor, integrated liquid water and rainfall rate derived from the Seasat Scanning Multichannel Microwave Radiometer (SMMR) during a September 10-12, 1978 North Pacific cyclone are studied. These patterns are compared with surface analyses, ship reports, radiosonde data, and GOES-West infrared satellite imagery. The SMMR data give a unique view of the large mesoscale structure of a midlatitude cyclone. The water vapor distribution is found to have characteristic patterns related to the location of the surface fronts throughout the development of the cyclone. An example is given to illustrate that SMMR data could significantly improve frontal analysis over data-sparse oceanic regions. The distribution of integrated liquid water agrees qualitatively well with corresponding cloud patterns in satellite imagery and appears to provide a means to distinguish where liquid water clouds exist under a cirrus shield. Ship reports of rainfall intensity agree qualitatively very well with SMMR-derived rainrates. Areas of mesoscale rainfall, on the order of 50 km x 50 km or greater are detected using SMMR derived rainrates.

  5. A brief history of 25 years (or more) of infrared imaging radiometers

    Science.gov (United States)

    Lyon, Bernard R., Jr.; Orlove, Gary L.

    2003-04-01

    Modern thermal imaging radiometers are infrared systems usually endowed with some means of making surface temperature measurements of objects, as well as providing an image. These devices have evolved considerably over the past few decades, and are continuing to do so at an accelerating rate. Changes are not confined to merely camera size and user interface, but also include critical parameters, such as sensitivity, accuracy, dynamic range, spectral response, capture rates, storage media, and numerous other features, options, and accessories. Familiarity with this changing technology is much more than an academic topic. A misunderstanding or false assumption concerning system differences, could lead to misinterpretation of data, inaccurate temperature measurements, or disappointing, ambiguous results. Marketing demands have had considerable influence in the design and operation of these systems. In the past, many thermographers were scientists, engineers and researchers. Today, however, the majorities of people using these instruments work in the industrial sector and are involved in highly technical skilled trades. This change of operating personnel has effectively changed the status of these devices from a 'scientific instrument', to an 'essential tool'. Manufacturers have recognized this trend and responded accordingly, as seen in their product designs. This paper explores the history of commercial infrared imaging systems and accessories. Emphasis is placed on, but not confined to, real time systems with video output, capable of temperature measurements.

  6. The Benefits of Sample Return: Connecting Apollo Soils and Diviner Lunar Radiometer Remote Sensing Data

    Science.gov (United States)

    Greenhagen, B. T.; Donaldson-Hanna, K. L.; Thomas, I. R.; Bowles, N. E.; Allen, C. C.; Pieters, C. M.; Paige, D. A.

    2014-01-01

    The Diviner Lunar Radiometer, onboard NASA's Lunar Reconnaissance Orbiter, has produced the first global, high resolution, thermal infrared observations of an airless body. The Moon, which is the most accessible member of this most abundant class of solar system objects, is also the only body for which we have extraterrestrial samples with known spatial context. Here we present the results of a comprehensive study to reproduce an accurate simulated lunar environment, evaluate the most appropriate sample and measurement conditions, collect thermal infrared spectra of a representative suite of Apollo soils, and correlate them with Diviner observations of the lunar surface. We find that analyses of Diviner observations of individual sampling stations and SLE measurements of returned Apollo soils show good agreement, while comparisons to thermal infrared reflectance under terrestrial conditions do not agree well, which underscores the need for SLE measurements and validates the Diviner compositional dataset. Future work includes measurement of additional soils in SLE and cross comparisons with measurements in JPL Simulated Airless Body Emission Laboratory (SABEL).

  7. A multispectral study of an extratropical cyclone with Nimbus 3 medium resolution infrared radiometer data

    Science.gov (United States)

    Holub, R.; Shenk, W. E.

    1973-01-01

    Four registered channels (0.2 to 4, 6.5 to 7, 10 to 11, and 20 to 23 microns) of the Nimbus 3 Medium Resolution Infrared Radiometer (MRIR) were used to study 24-hr changes in the structure of an extratropical cyclone during a 6-day period in May 1969. Use of a stereographic-horizon map projection insured that the storm was mapped with a single perspective throughout the series and allowed the convenient preparation of 24-hr difference maps of the infrared radiation fields. Single-channel and multispectral analysis techniques were employed to establish the positions and vertical slopes of jetstreams, large cloud systems, and major features of middle and upper tropospheric circulation. Use of these techniques plus the difference maps and continuity of observation allowed the early detection of secondary cyclones developing within the circulation of the primary cyclone. An automated, multispectral cloud-type identification technique was developed, and comparisons that were made with conventional ship reports and with high-resolution visual data from the image dissector camera system showed good agreement.

  8. Use of radiometer to reform and repair an old living house to passive solar one

    Science.gov (United States)

    Okamoto, Yoshizo; Inagaki, Terumi; Suzuki, Takakazu; Kurokawa, Takashi

    1994-03-01

    Japanese living houses mainly consist of wooden elements in high-temperature and moist conditions. To modify the hot and humid environment, a conventional old house was partially rebuilt and repaired. Especially in the winter season, a diagnostic thermographic test was used to find deteriorated and leaking parts of interior and exterior walls. Macroscopic deteriorated parts were checked again in detail. The deteriorated element was then removed. During the reconstruction process, a new solar heat and air conditioning system using a silica-gel adsorber and underground water was installed to cool and warm up the living room. Thermography tests of this remodeled house show that room temperature is always constant and mild to human beings, especially in the winter. Temperature and heat flow distribution of flowing air in the living room was measured using thermal net and wire methods. Leaking thermal streak flow of the gap was locally visualized by the IR radiometer and a highly sensitive video camera. It was verified that IR thermography is a useful measuring instrument to check thermal defects of a house.

  9. Re-thinking the role of radiometal isotopes: Towards a future concept for theranostic radiopharmaceuticals.

    Science.gov (United States)

    Notni, Johannes; Wester, Hans-Jürgen

    2018-03-01

    The potential and future role of certain metal radionuclides, for example, 44 Sc, 89 Zr, 86 Y, 64 Cu, 68 Ga, 177 Lu, 225 Ac, and 213 Bi, and several terbium isotopes has been controversially discussed in the past decades. Furthermore, the possible benefits of "matched pairs" of isotopes for tandem applications of diagnostics and therapeutics (theranostics) have been emphasized, while such approaches still have not made their way into routine clinical practice. Analysis of bibliographical data illustrates how popularity of certain nuclides has been promoted by cycles of availability and applications. We furthermore discuss the different practical requirements for diagnostic and therapeutic radiopharmaceuticals and the resulting consequences for efficient development of clinically useful pairs of radionuclide theranostics, with particular emphasis on the underlying economical factors. Based on an exemplary assessment of overall production costs for 68 Ga and 18 F radiopharmaceuticals, we venture a look into the future of theranostics and predict that high-throughput PET applications, that is, diagnosis of frequent conditions, will ultimately rely on 18 F tracers. PET radiometals will occupy a niche in the clinical low-throughput sector (diagnosis of rare diseases), but above all, dominate preclinical research and clinical translation. Matched isotope pairs will be of lesser relevance for theranostics but may become important for future PET-based therapeutic dosimetry. Copyright © 2017 John Wiley & Sons, Ltd.

  10. Antibodies to watch in 2018

    Science.gov (United States)

    Kaplon, Hélène; Reichert, Janice M.

    2018-01-01

    ABSTRACT The pace of antibody therapeutics development accelerated in 2017, and this faster pace is projected to continue through 2018. Notably, the annual number of antibody therapeutics granted a first approval in either the European Union (EU) or United States (US) reached double-digits (total of 10) for the first time in 2017. The 10 antibodies granted approvals are: brodalumab, dupilumab, sarilumab, guselkumab, benralizumab, ocrelizumab, inotuzumab ozogamicin, avelumab, duvalumab, and emicizumab. Brodalumab, however, had already been approved in Japan in 2016. As of December 1, 2017, nine antibody therapeutics (ibalizumab, burosumab, tildrakizumab, caplacizumab, erenumab, fremanezumab, galcanezumab, romosozumab, mogamulizumab) were in regulatory review in the EU or US, and regulatory actions on their marketing applications are expected by the end of 2018. Based on company announcements and estimated clinical study primary completion dates, and assuming the study results are positive, marketing applications for at least 12 antibody therapeutics that are now being evaluated in late-stage clinical studies may be submitted by the end of 2018. Of the 12 candidates, 8 are for non-cancer indications (lanadelumab, crizanlizumab, ravulizumab, eptinezumab, risankizumab, satralizumab, brolucizumab, PRO140) and 4 are for cancer (sacituzumab govitecan, moxetumomab pasudotox, cemiplimab, ublituximab). Additional antibody therapeutics to watch in 2018 include 19 mAbs undergoing evaluation in late-stage studies with primary completion dates in late 2017 or during 2018. Of these mAbs, 9 are for non-cancer indications (lampalizumab, roledumab, emapalumab, fasinumab, tanezumab, etrolizumab, NEOD001, gantenerumab, anifrolumab) and 10 are for cancer indications (tremelimumab, isatuximab, BCD-100, carotuximab, camrelizumab, IBI308, glembatumumab vedotin, mirvetuximab soravtansine, oportuzumab monatox, L19IL2/L19TNF). Positive clinical study results may enable marketing application

  11. [Current Treatment of Stable Angina].

    Science.gov (United States)

    Toggweiler, Stefan; Jamshidi, Peiman; Cuculi, Florim

    2015-06-17

    Current therapy for stable angina includes surgical and percutaneous revascularization, which has been improved tremendously over the last decades. Smoking cessation and regular exercise are the cornerstone for prevention of further cerebrovascular events. Medical treatment includes treatment of cardiovascular risk factors and antithrombotic management, which can be a challenge in some patients. Owing to the fact the coronary revascularization is readily accessible these days in many industrialized countries, the importance of antianginal therapy has decreased over the past years. This article presents a contemporary overview of the management of patients with stable angina in the year 2015.

  12. Calibration of the degree of linear polarization measurements of the polarized Sun-sky radiometer based on the POLBOX system.

    Science.gov (United States)

    Li, Zhengqiang; Li, Kaitao; Li, Li; Xu, Hua; Xie, Yisong; Ma, Yan; Li, Donghui; Goloub, Philippe; Yuan, Yinlin; Zheng, Xiaobing

    2018-02-10

    Polarization observation of sky radiation is the frontier approach to improve the remote sensing of atmospheric components, e.g., aerosol and clouds. The polarization calibration of the ground-based Sun-sky radiometer is the basis for obtaining accurate degree of linear polarization (DOLP) measurement. In this paper, a DOLP calibration method based on a laboratory polarized light source (POLBOX) is introduced in detail. Combined with the CE318-DP Sun-sky polarized radiometer, a calibration scheme for DOLP measurement is established for the spectral range of 440-1640 nm. Based on the calibration results of the Sun-sky radiometer observation network, the polarization calibration coefficient and the DOLP calibration residual are analyzed statistically. The results show that the DOLP residual of the calibration scheme is about 0.0012, and thus it can be estimated that the final DOLP calibration accuracy of this method is about 0.005. Finally, it is verified that the accuracy of the calibration results is in accordance with the expected results by comparing the simulated DOLP with the vector radiative transfer calculations.

  13. A novel fast-scanning microwave heterodyne radiometer system for electron cyclotron emission measurements in the HT-7 superconducting tokamak

    International Nuclear Information System (INIS)

    Zhang, S.Y.; Wan, Y.X.; Xie, J.K.; Luo, J.R.; Li, J.G.; Kuang, G.L.; Gao, X.; Zhang, X.D.; Wan, B.N.; Wang, K.J.; Mao, J.S.; Gong, X.Z.; Qin, P.J.

    2000-01-01

    Two sets of fast-scanning microwave heterodyne radiometer receiver systems employing backward-wave oscillators in the 78-118 GHz and 118-178 GHz ranges were developed for electron cyclotron emission measurements (ECE) on the HT-7 superconducting tokamak. The double-sideband radiometer in the 78-118 GHz range measures 16 ECE frequency points with a scanning period of 0.65 ms. The novel design of the 2 mm fast-scanning heterodyne radiometer in the 118-178 GHz range enables the unique system to measure 48 ECE frequency points in 0.65 ms periodically. The plasma profile consistency in reproducible ohmic plasmas was used to relatively calibrate each channel by changing the toroidal magnetic field shot-by-shot. The absolute temperature value was obtained by a comparison with the results from the soft x-ray pulse height analysis measurements and Thomson scattering system. A preliminary temperature profile measurement result in pellet injection plasma is presented. (author)

  14. PAU/RAD: Design and Preliminary Calibration Results of a New L-Band Pseudo-Correlation Radiometer Concept

    Directory of Open Access Journals (Sweden)

    Enric Valencia

    2008-07-01

    Full Text Available The Passive Advanced Unit (PAU for ocean monitoring is a new type of instrument that combines in a single receiver and without time multiplexing, a polarimetric pseudo-correlation microwave radiometer at L-band (PAU-RAD and a GPS reflectometer (PAU-GNSS/R. These instruments in conjunction with an infra-red radiometer (PAU-IR will respectively provide the sea surface temperature and the sea state information needed to accurately retrieve the sea surface salinity from the radiometric measurements. PAU will consist of an array of 4x4 receivers performing digital beamforming and polarization synthesis both for PAU-RAD and PAU-GNSS/R. A concept demonstrator of the PAU instrument with only one receiver has been implemented (PAU-One Receiver or PAU-OR. PAU-OR has been used to test and tune the calibration algorithms that will be applied to PAU. This work describes in detail PAU-OR’s radiometer calibration algorithms and their performance.

  15. Design and characterization of the Large-aperture Experiment to Detect the Dark Age (LEDA) radiometer systems

    Science.gov (United States)

    Price, D. C.; Greenhill, L. J.; Fialkov, A.; Bernardi, G.; Garsden, H.; Barsdell, B. R.; Kocz, J.; Anderson, M. M.; Bourke, S. A.; Craig, J.; Dexter, M. R.; Dowell, J.; Eastwood, M. W.; Eftekhari, T.; Ellingson, S. W.; Hallinan, G.; Hartman, J. M.; Kimberk, R.; Lazio, T. Joseph W.; Leiker, S.; MacMahon, D.; Monroe, R.; Schinzel, F.; Taylor, G. B.; Tong, E.; Werthimer, D.; Woody, D. P.

    2018-05-01

    The Large-Aperture Experiment to Detect the Dark Age (LEDA) was designed to detect the predicted O(100) mK sky-averaged absorption of the Cosmic Microwave Background by Hydrogen in the neutral pre- and intergalactic medium just after the cosmological Dark Age. The spectral signature would be associated with emergence of a diffuse Lyα background from starlight during `Cosmic Dawn'. Recently, Bowman et al. (2018) have reported detection of this predicted absorption feature, with an unexpectedly large amplitude of 530 mK, centered at 78 MHz. Verification of this result by an independent experiment, such as LEDA, is pressing. In this paper, we detail design and characterization of the LEDA radiometer systems, and a first-generation pipeline that instantiates a signal path model. Sited at the Owens Valley Radio Observatory Long Wavelength Array, LEDA systems include the station correlator, five well-separated redundant dual polarization radiometers and backend electronics. The radiometers deliver a 30-85 MHz band (16 z < 34) and operate as part of the larger interferometric array, for purposes ultimately of in situ calibration. Here, we report on the LEDA system design, calibration approach, and progress in characterization as of January 2016. The LEDA systems are currently being modified to improve performance near 78 MHz in order to verify the purported absorption feature.

  16. Monoclonal antibodies technology. Protocols

    International Nuclear Information System (INIS)

    Acevado Castro, B.E.

    1997-01-01

    Full text: Immunization. The first step in preparing useful monoclonal antibodies (MAbs) is to immunize an animal (Balb/c for example) with an appropriate antigen. Methods (only for soluble antigen): Solubilize selected antigen in Phosphate buffer solution (PBS) at pH 7.2-7.4, ideally at a final concentration per animal between 10 to 50 μg/ml. It is recommended that the antigen under consideration be incorporated into the emulsion adjuvants in 1:1 volumetric relation. We commonly use Frend's adjuvant (FA) to prepared immunized solution. The first immunization should be prepared with complete FA, and the another could be prepared with incomplete FA. It is recommended to inject mice with 0.2 ml intraperitoneal (ip) or subcutaneous (sc). Our experience suggests the sc route is the preferred route. A minimum protocol for immunizing mice to generate cells for preparing hybridomas is s follows: immunize sc on day 0, boost sc on day 21, take a trial bleeding on day 26; if antibody titters are satisfactory, boost ip on day 35 with antigen only, and remove the spleen to obtain cells for fusion on day 38. Fusion protocol. The myeloma cell line we are using is X63 Ag8.653. At the moment of fusion myeloma cells need a good viability (at least a 95%). 1. Remove the spleen cells from immunized mice using sterile conditions. An immune spleen should yield between 7 a 10x10 7 nucleated cells. 2. Place the spleen in 20 ml of serum-free RPMI 1640 in a Petri dish. Using a needle and syringe, inject the spleen with medium to distend and disrupt the spleen stroma and free the nucleated cells. 3. Flush the cell suspension with a Pasteur pipet to disperse clumps of cells. 4. Centrifuge the spleen cell suspension at 250g for 10 min. Resuspend the pellet in serum-free RPMI 1640. Determine cell concentration using Neuhabuer chamber. 5. Mix the myeloma cells and spleen cells in a conical 50-ml tube in serum-free RPMI 1640, 1 x10 7 spleen cells to 1x10 6 myeloma cells (ratio 10:1). Centrifuge

  17. Tabhu: tools for antibody humanization.

    KAUST Repository

    Olimpieri, Pier Paolo

    2014-10-09

    SUMMARY: Antibodies are rapidly becoming essential tools in the clinical practice, given their ability to recognize their cognate antigens with high specificity and affinity, and a high yield at reasonable costs in model animals. Unfortunately, when administered to human patients, xenogeneic antibodies can elicit unwanted and dangerous immunogenic responses. Antibody humanization methods are designed to produce molecules with a better safety profile still maintaining their ability to bind the antigen. This can be accomplished by grafting the non-human regions determining the antigen specificity into a suitable human template. Unfortunately, this procedure may results in a partial or complete loss of affinity of the grafted molecule that can be restored by back-mutating some of the residues of human origin to the corresponding murine ones. This trial-and-error procedure is hard and involves expensive and time-consuming experiments. Here we present tools for antibody humanization (Tabhu) a web server for antibody humanization. Tabhu includes tools for human template selection, grafting, back-mutation evaluation, antibody modelling and structural analysis, helping the user in all the critical steps of the humanization experiment protocol. AVAILABILITY: http://www.biocomputing.it/tabhu CONTACT: anna.tramontano@uniroma1.it, pierpaolo.olimpieri@uniroma1.it SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

  18. Antibodies to watch in 2014.

    Science.gov (United States)

    Reichert, Janice M

    2014-01-01

    Since 2010, mAbs has documented the biopharmaceutical industry's progress in transitioning antibody therapeutics to first Phase 3 clinical studies and regulatory review, and its success at gaining first marketing approvals for antibody-based products. This installment of the "Antibodies to watch" series outlines events anticipated to occur between December 2013 and the end of 2014, including first regulatory actions on marketing applications for vedolizumab, siltuximab, and ramucirumab, as well as the Fc fusion proteins Factor IX-Fc and Factor VIII-Fc; and the submission of first marketing applications for up to five therapeutics (secukinumab, ch14.18, onartuzumab, necitumumab, gevokizumab). Antibody therapeutics in Phase 3 studies are described, with an emphasis on those with study completion dates in 2014, including antibodies targeting interleukin-17a or the interleukin-17a receptor (secukinumab, ixekizumab, brodalumab), proprotein convertase subtilisin/kexin type 9 (alirocumab, evolocumab, bococizumab), and programmed death 1 receptor (lambrolizumab, nivolumab). Five antibodies with US Food and Drug Administration's Breakthrough Therapy designation (obinutuzumab, ofatumumab, lambrolizumab, bimagrumab, daratumumab) are also discussed.

  19. Avian Diagnostic and Therapeutic Antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Bradley, David Sherman [UND SMHS

    2012-12-31

    A number of infectious agents have the potential of causing significant clinical symptomology and even death, but dispite this, the number of incidence remain below the level that supports producing a vaccine. Therapeutic antibodies provide a viable treatment option for many of these diseases. We proposed that antibodies derived from West Nile Virus (WNV) immunized geese would be able to treat WNV infection in mammals and potential humans. We demonstrated that WNV specific goose antibodies are indeed successful in treating WNV infection both prophylactically and therapeutically in a golden hamster model. We demonstrated that the goose derived antibodies are non-reactogenic, i.e. do not cause an inflammatory response with multiple exposures in mammals. We also developed both a specific pathogen free facility to house the geese during the antibody production phase and a patent-pending purification process to purify the antibodies to greater than 99% purity. Therefore, the success of these study will allow a cost effective rapidly producible therapeutic toward clinical testing with the necessary infrastructure and processes developed and in place.

  20. Fluctuation of zonulin levels in blood vs stability of antibodies.

    Science.gov (United States)

    Vojdani, Aristo; Vojdani, Elroy; Kharrazian, Datis

    2017-08-21

    To evaluate the measurement of zonulin level and antibodies of zonulin and other tight junction proteins in the blood of controls and celiac disease patients. This study was conducted to assess the variability or stability of zonulin levels vs IgA and IgG antibodies against zonulin in blood samples from 18 controls at 0, 6, 24 and 30 h after blood draw. We also measured zonulin level as well as zonulin, occludin, vinculin, aquaporin 4 and glial fibrillary acidic protein antibodies in the sera of 30 patients with celiac disease and 30 controls using enzyme-linked immunosorbent assay methodology. The serum zonulin level in 6 out of 18 subjects was low or zonulin levels of > 2.8 ng/mL and showed significant fluctuation from sample to sample. Comparatively, zonulin antibody measured in all samples was highly stable and reproducible from sample to sample. Celiac disease patients showed zonulin levels with a mean of 8.5 ng/mL compared to 3.7 ng/mL in controls ( P zonulin level at 2SD above the mean was demonstrated in 37% of celiac disease patients, while antibodies against zonulin, occludin and other tight junction proteins was detected in up to 86% of patients with celiac disease. Due to its fluctuation, a single measurement of zonulin level is not recommended for assessment of intestinal barrier integrity. Measurement of IgG and IgA antibodies against zonulin, occludin, and other tight junction proteins is proposed for the evaluation of the loss of intestinal barrier integrity.