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Sample records for spires image hep

  1. SLAC SPIRES HEP database

    CERN Document Server

    Contains over 400,000 bibliographic summaries for particle physics papers (e-prints, journal articles, preprints, reports, theses, etc.). Covers 1974 to the present and is updated daily with links to electronic texts ( e.g. from LANL, CERN, KEK, and other HEP servers). Searchable by all authors and authors' affiliations, title, topic, report number, citation (footnotes), e-print archive number, date, journal, etc. A joint project of the SLAC and DESY libraries with the collaboration of many other research institutions and scholarly societies such as the APS.

  2. The Herschel-SPIRE Imaging FTS Pipeline: Testing the Deglitching Modules

    Science.gov (United States)

    Benielli, D.; Fulton, T. R.; Baluteau, J.; Davis-Imhof, P.; Surace, C.; Litven, J.; Kennedy, P.; Naylor, D. A.; Clements, D.; Gastaud, R.; Panuzzo, P.; Polehampton, E.; Leeks, S. J.; Lim, T. L.; Guest, S.; Swinyard, B. M.; Lu, N.; Schwartz, A.; Griffin, M.; Xu, K.

    2009-09-01

    SPIRE, the Spectral and Photometric Imaging Receiver, is one of three science instruments (SPIRE, PACS, HIFI) observing in the 200-670 micron wavelength range. SPIRE is a Fourier transform spectrometer in Mach-Zehnder configuration. The pipeline for the SPIRE imaging spectrometer is integrated into the Herschel Common Science System, which is based on the Java programming language. This paper focuses on the development and validation of the two deglitching modules within that pipeline.

  3. Performance evaluation of the Herschel/SPIRE imaging Fourier transform spectrometer through ground-based measurements

    Science.gov (United States)

    Spencer, Locke D.; Naylor, David A.; Swinyard, Bruce M.

    2010-06-01

    The Spectral and Photometric Imaging Receiver (SPIRE) is one of three scientific instruments onboard the European Space Agency (ESA)'s Herschel Space Observatory. Herschel was successfully launched on 14 May 2009; routine science observations commenced in late 2009. Medium resolution spectroscopy with SPIRE is accomplished via an imaging Fourier transform spectrometer (IFTS) of the Mach-Zehnder configuration. Although pre-launch performance verification and calibration measurements were conducted with the SPIRE instrument mounted in an evacuated cryostat at cryogenic temperatures, it was not possible to simulate fully the expected in-flight conditions. This paper compares the performance of the SPIRE IFTS, as measured during ground-based tests, with theoretical simulations. In turn, these results are used to provide an estimate of the in-flight instrument performance. This paper includes a discussion of key aspects of the SPIRE IFTS including the spectrometer dual-input compensation scheme, instrument line shape and the overall instrument sensitivity. As a case study, the derived instrument performance is used to investigate SPIRE's utility in observing astronomical line emission from the starburst galaxy M82.

  4. HEP technologies to address medical imaging challenges

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    Developments in detector technologies aimed at solving challenges in present and future CERN experiments, particularly at the LHC, have triggered exceptional advances in the performance of medical imaging devices, allowing for a spectacular progress in in-vivo molecular imaging procedures, which are opening the way for tailored therapies of major diseases. This talk will briefly review the recent history of this prime example of technology transfer from HEP experiments to society, will describe the technical challenges being addressed by some ongoing projects, and will present a few new ideas for further developments and their foreseeable impact.

  5. Spherical Panoramas, and non Metric Images for Long Range Survey, the San Barnaba Spire, Sagrada Familia, Barcelona, Spain

    Directory of Open Access Journals (Sweden)

    Elisa Cingolani

    2011-12-01

    consist basically in the difficulty given by its height above the street level, about 100 meters. Long focal lenses have to be used to get a suitable resolution and accuracy. We wanted to repeat now the survey using a different photogrammetric technique from the old one, that was DLT algorithm for non-metric images. The new technique is the Spherical Photogrammetry. Multi-image Spherical Photogrammetry makes use as sensor of a pseudo-image that is the spherical panorama, composed by the images taken from the same station point. For details of Spherical Photogrammetry see (Fangi, 2,3,4,9. A particular procedure appropriate for the orientation of very narrow field of view lenses panorama has been already set up and used for the orientation and plotting of the three minarets of the Great Mosque of Omayyad’s in Damascus, Syria. Their heights range from 60 to 80 meters above the courtyard pavement of the mosque. The technique consists in taking different focal lengths panorama from the same station point (Fangi, New Castle, 2010, one with WA wide angle and another one with NA Narrow Angle, adding to the stability of WA panorama the resolution of NA panorama. The same approach has been used in the Sagrada Familia, for the survey of San Barnaba’s spire. In 1990 the A. made a survey of the same spire. But in comparison to the years 90, there is one difficulty more: now the rear of the spire is not visible because of the construction of the roof of the church, while it was visible in 1990. The solution has been then to use the original images taken in the years 90 for the rear of the spire and the spherical panoramas for the rest, i.e. the part toward the façade, using the original control points. Then we had to make a combined adjustment of non metric images using DLT approach and spherical photogrammetry algorithms. The restitution has been indeed carried out using both type of imagery, spherical panorama and non metric images. The results are satisfactory in the sense that

  6. Update History of This Database - DB-SPIRE | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...List Contact us DB-SPIRE Update History of This Database Date Update contents 2017/02/16 DB-SPIRE English ar...ase Description Download License Update History of This Database Site Policy | Contact Us Update History of This Database - DB-SPIRE | LSDB Archive ...

  7. Feature Importance for Human Epithelial (HEp-2 Cell Image Classification

    Directory of Open Access Journals (Sweden)

    Vibha Gupta

    2018-02-01

    Full Text Available Indirect Immuno-Fluorescence (IIF microscopy imaging of human epithelial (HEp-2 cells is a popular method for diagnosing autoimmune diseases. Considering large data volumes, computer-aided diagnosis (CAD systems, based on image-based classification, can help in terms of time, effort, and reliability of diagnosis. Such approaches are based on extracting some representative features from the images. This work explores the selection of the most distinctive features for HEp-2 cell images using various feature selection (FS methods. Considering that there is no single universally optimal feature selection technique, we also propose hybridization of one class of FS methods (filter methods. Furthermore, the notion of variable importance for ranking features, provided by another type of approaches (embedded methods such as Random forest, Random uniform forest is exploited to select a good subset of features from a large set, such that addition of new features does not increase classification accuracy. In this work, we have also, with great consideration, designed class-specific features to capture morphological visual traits of the cell patterns. We perform various experiments and discussions to demonstrate the effectiveness of FS methods along with proposed and a standard feature set. We achieve state-of-the-art performance even with small number of features, obtained after the feature selection.

  8. SPIRES I: on-line search guide

    International Nuclear Information System (INIS)

    Addis, L.

    1975-06-01

    SPIRES I is the first generation of the on-line Stanford Public Information Retrieval System. Designed as a prototype system, SPIRES I was later moved to the SLAC computing facility where it has been routinely available to SLAC users in the field of high-energy physics. The scope and use of the SPIRES I system are described in this manual

  9. HEp-2 Cell Image Classification With Deep Convolutional Neural Networks.

    Science.gov (United States)

    Gao, Zhimin; Wang, Lei; Zhou, Luping; Zhang, Jianjia

    2017-03-01

    Efficient Human Epithelial-2 cell image classification can facilitate the diagnosis of many autoimmune diseases. This paper proposes an automatic framework for this classification task, by utilizing the deep convolutional neural networks (CNNs) which have recently attracted intensive attention in visual recognition. In addition to describing the proposed classification framework, this paper elaborates several interesting observations and findings obtained by our investigation. They include the important factors that impact network design and training, the role of rotation-based data augmentation for cell images, the effectiveness of cell image masks for classification, and the adaptability of the CNN-based classification system across different datasets. Extensive experimental study is conducted to verify the above findings and compares the proposed framework with the well-established image classification models in the literature. The results on benchmark datasets demonstrate that 1) the proposed framework can effectively outperform existing models by properly applying data augmentation, 2) our CNN-based framework has excellent adaptability across different datasets, which is highly desirable for cell image classification under varying laboratory settings. Our system is ranked high in the cell image classification competition hosted by ICPR 2014.

  10. HEp-2 cell image classification method based on very deep convolutional networks with small datasets

    Science.gov (United States)

    Lu, Mengchi; Gao, Long; Guo, Xifeng; Liu, Qiang; Yin, Jianping

    2017-07-01

    Human Epithelial-2 (HEp-2) cell images staining patterns classification have been widely used to identify autoimmune diseases by the anti-Nuclear antibodies (ANA) test in the Indirect Immunofluorescence (IIF) protocol. Because manual test is time consuming, subjective and labor intensive, image-based Computer Aided Diagnosis (CAD) systems for HEp-2 cell classification are developing. However, methods proposed recently are mostly manual features extraction with low accuracy. Besides, the scale of available benchmark datasets is small, which does not exactly suitable for using deep learning methods. This issue will influence the accuracy of cell classification directly even after data augmentation. To address these issues, this paper presents a high accuracy automatic HEp-2 cell classification method with small datasets, by utilizing very deep convolutional networks (VGGNet). Specifically, the proposed method consists of three main phases, namely image preprocessing, feature extraction and classification. Moreover, an improved VGGNet is presented to address the challenges of small-scale datasets. Experimental results over two benchmark datasets demonstrate that the proposed method achieves superior performance in terms of accuracy compared with existing methods.

  11. Herschel/SPIRE observations of the dusty disk of NGC 4244

    NARCIS (Netherlands)

    Holwerda, B. W.; Bianchi, S.; Boker, T.; Radburn-Smith, D.; de Jong, R. S.; Baes, M.; van der Kruit, P. C.; Xilouris, M.; Gordon, K. D.; Dalcanton, J. J.

    We present Herschel/SPIRE images at 250, 350, and 500 mu m of NGC 4244, a typical low-mass, disk-only and edge-on spiral galaxy. The dust disk is clumpy and shows signs of truncation at the break radius of the stellar disk. This disk coincides with the densest part of the Hi disk. We compare the

  12. [Preparation of a glypican-3-targeting hepatocellular carcinoma MR probe and its molecular imaging in HepG2 cells].

    Science.gov (United States)

    Gu, Yan; Zeng, Yan; Guo, Da-Jing; Yang, Jing; Zhou, Jun; Liu, Xin-Jie; Wang, Zhi-Gang

    2014-02-01

    To prepare a glypican-3 (GPC3)-targeting hepatocellular carcinoma MR molecular probe and to evaluate its targeting specificity using HepG2 cells. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles were prepared by a double emulsion solvent evaporation method, and the surfaces were connected with anti-GPC3 mono-antibody and paramagnetic substance Gd3+. The physical properties of the probes were investigated using fluorescence microscopy, electron microscopy, Malvern particle size analysis, inductively coupled plasma atomic emission spectroscopy (ICP-AES) and 1.5T MR imaging. The specificity of the probes to target cultured HepG2 cells was determined by laser confocal microscopy. The signal characteristics, including signal-to-noise ratio (SNR), after co-incubation with HepG2 cells were analyzed by 1.5T MR imaging. Significance of differences between multiple groups (target group, non-target group, and control group) was assessed by one-way analysis of variance, and between two groups was assessed by the LSD-t test. A difference was considered to be statistically significant at P less than 0.05. The GPC3-targeting hepatocellular carcinoma MR molecular probes were successfully prepared. The nanoparticles had a spherical shape, size of 495 +/- 17.5 nm, uniform size distribution, good dispersibility, no obvious aggregation, and could significantly increase the T1 signal. Using the ICP-AES measurement, 1 mol PLGA carried about 12 mol Gd3+, and as the Gd3+ concentration increased, the T1 signal increased. The prepared MR molecular probes could specifically target HepG2 cells, and could increase the T1 signal. The SNR value of the target group was 3.45 +/- 0.21, of the non-target group was 1.43 +/- 0.07, and of the control group was 1.12 +/- 0.03. The SNR value of the target group was significantly higher than that of the non-target group and the control group (P less than 0.05); there was no significant difference between the non-target group and the control group (P more

  13. Targeting and molecular imaging of HepG2 cells using surface-functionalized gold nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Rathinaraj, Pierson [Auckland University of Technology, Institute of Biomedical Technologies (New Zealand); Lee, Kyubae; Choi, Yuri; Park, Soo-Young [Kyungpook National University, School of Applied Chemical Engineering, Graduate School (Korea, Republic of); Kwon, Oh Hyeong [Kumoh National Institute of Technology, Department of Polymer Science and Engineering (Korea, Republic of); Kang, Inn-Kyu, E-mail: ikkang@knu.ac.kr [Kyungpook National University, School of Applied Chemical Engineering, Graduate School (Korea, Republic of)

    2015-07-15

    Mercaptosuccinic acid (M)-conjugated gold nanoparticles (GM) were prepared and characterized by transmission electron microscope and dynamic light scattering. M was used to improve the monodispersity and non-specific intracellular uptake of nanoparticles. Lactobionic acid (L) was subsequently conjugated to the GM to target preferentially HepG2 cells (liver cancer cells) that express asialoglycoprotein receptors (ASGPR) on their membrane surfaces and facilitate the transit of nanoparticles across the cell membrane. The mean size of lactobionic acid-conjugated gold nanoparticle (GL) was approximately 10 ± 0.2 nm. Finally, the Atto 680 dye (A6) was coupled to the nanoparticles to visualize their internalization into HepG2 cells. The interaction of surface-modified gold nanoparticles with HepG2 cells was studied after culturing cells in media containing the GM or L-conjugated GM (GL)

  14. Imaging-Based Screen Identifies Laminin 411 as a Physiologically Relevant Niche Factor with Importance for i-Hep Applications.

    Science.gov (United States)

    Ong, John; Serra, Maria Paola; Segal, Joe; Cujba, Ana-Maria; Ng, Soon Seng; Butler, Richard; Millar, Val; Hatch, Stephanie; Zimri, Salman; Koike, Hiroyuki; Chan, Karen; Bonham, Andrew; Walk, Michelle; Voss, Ty; Heaton, Nigel; Mitry, Ragai; Dhawan, Anil; Ebner, Daniel; Danovi, Davide; Nakauchi, Hiromitsu; Rashid, S Tamir

    2018-03-13

    Use of hepatocytes derived from induced pluripotent stem cells (i-Heps) is limited by their functional differences in comparison with primary cells. Extracellular niche factors likely play a critical role in bridging this gap. Using image-based characterization (high content analysis; HCA) of freshly isolated hepatocytes from 17 human donors, we devised and validated an algorithm (Hepatocyte Likeness Index; HLI) for comparing the hepatic properties of cells against a physiological gold standard. The HLI was then applied in a targeted screen of extracellular niche factors to identify substrates driving i-Heps closer to the standard. Laminin 411, the top hit, was validated in two additional induced pluripotent stem cell (iPSC) lines, primary tissue, and an in vitro model of α1-antitrypsin deficiency. Cumulatively, these data provide a reference method to control and screen for i-Hep differentiation, identify Laminin 411 as a key niche protein, and underscore the importance of combining substrates, soluble factors, and HCA when developing iPSC applications. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

  15. Learning Boolean Networks in HepG2 cells using ToxCast High-Content Imaging Data (SOT annual meeting)

    Science.gov (United States)

    Cells adapt to their environment via homeostatic processes that are regulated by complex molecular networks. Our objective was to learn key elements of these networks in HepG2 cells using ToxCast High-content imaging (HCI) measurements taken over three time points (1, 24, and 72h...

  16. Correcting the extended-source calibration for the Herschel-SPIRE Fourier-transform spectrometer

    Science.gov (United States)

    Valtchanov, I.; Hopwood, R.; Bendo, G.; Benson, C.; Conversi, L.; Fulton, T.; Griffin, M. J.; Joubaud, T.; Lim, T.; Lu, N.; Marchili, N.; Makiwa, G.; Meyer, R. A.; Naylor, D. A.; North, C.; Papageorgiou, A.; Pearson, C.; Polehampton, E. T.; Scott, J.; Schulz, B.; Spencer, L. D.; van der Wiel, M. H. D.; Wu, R.

    2018-03-01

    We describe an update to the Herschel-Spectral and Photometric Imaging Receiver (SPIRE) Fourier-transform spectrometer (FTS) calibration for extended sources, which incorporates a correction for the frequency-dependent far-field feedhorn efficiency, ηff. This significant correction affects all FTS extended-source calibrated spectra in sparse or mapping mode, regardless of the spectral resolution. Line fluxes and continuum levels are underestimated by factors of 1.3-2 in thespectrometer long wavelength band (447-1018 GHz; 671-294 μm) and 1.4-1.5 in the spectrometer short wavelength band (944-1568 GHz; 318-191 μm). The correction was implemented in the FTS pipeline version 14.1 and has also been described in the SPIRE Handbook since 2017 February. Studies based on extended-source calibrated spectra produced prior to this pipeline version should be critically reconsidered using the current products available in the Herschel Science Archive. Once the extended-source calibrated spectra are corrected for ηff, the synthetic photometry and the broad-band intensities from SPIRE photometer maps agree within 2-4 per cent - similar levels to the comparison of point-source calibrated spectra and photometry from point-source calibrated maps. The two calibration schemes for the FTS are now self-consistent: the conversion between the corrected extended-source and point-source calibrated spectra can be achieved with the beam solid angle and a gain correction that accounts for the diffraction loss.

  17. Neoplasias hepáticas: caracterização por métodos de imagem Liver neoplasms: imaging characterization

    Directory of Open Access Journals (Sweden)

    Dario Ariel Tiferes

    2008-04-01

    Full Text Available Uma grande variedade de tumores benignos e malignos ocorre no fígado. Embora a caracterização de lesões hepáticas focais possa ser um desafio para o radiologista, a maioria das lesões se apresenta com características de imagem que permitem o seu diagnóstico. O objetivo deste trabalho é o de rever os principais aspectos de imagem dos tumores hepáticos benignos e malignos mais comumente encontrados no fígado adulto.A wide range of both benign and malignant neoplasms may occur in the liver. Although the characterization of focal hepatic lesions may represent a diagnostic challenge for radiologists, typical imaging findings in these lesions allow a correct diagnosis. The present study is aimed at reviewing imaging findings both in the most frequent benign and malignant focal lesion found in the adult liver.

  18. Spire's 3U CubeSat GNSS-RO Constellation for Meteorological and Space Weather Applications

    Science.gov (United States)

    Nguyen, V.; Duly, T.; Ector, D.; Irisov, V.; Nogues-Correig, O.; Tan, L.; Yuasa, T.

    2017-12-01

    Spire Global, Inc., is a leading player in the nanosatellite sector and the first commercial company to provide GNSS radio occultation measurements to support meteorological and space weather forecasting. Each Spire satellite is equipped with a state-of-the-art, in-house designed software receiver, which is capable of open-loop tracking of occulted GNSS signals. By utilizing this receiver on a low-earth orbiting, 3U satellite constellation platform, Spire is able to provide high-quality profile measurements of the lower atmosphere as well as ionospheric total electron content and scintillation data at unprecedented low cost, coverage, and latency. In this talk, we provide an overview of the current capabilities of Spire's satellite constellation and radio occultation processing system. Recent results describing the state of the lower atmosphere and ionosphere will be presented and briefly discussed. Finally, we focus on Spire's future capabilities, and the potential impacts on both the meteorological and space weather scientific communities.

  19. INSPIRE: A new scientific information system for HEP

    International Nuclear Information System (INIS)

    Ivanov, R; Raae, L

    2010-01-01

    The status of high-energy physics (HEP) information systems has been jointly analyzed by the libraries of CERN, DESY, Fermilab and SLAC. As a result, the four laboratories have started the INSPIRE project - a new platform built by moving the successful SPIRES features and content, curated at DESY, Fermilab and SLAC, into the open-source CDS Invenio digital library software that was developed at CERN. INSPIRE will integrate current acquisition workflows and databases to host the entire body of the HEP literature (about one million records), aiming to become the reference HEP scientific information platform worldwide. It will provide users with fast access to full text journal articles and preprints, but also material such as conference slides and multimedia. INSPIRE will empower scientists with new tools to discover and access the results most relevant to their research, enable novel text- and data-mining applications, and deploy new metrics to assess the impact of articles and authors. In addition, it will introduce the 'Web 2.0' paradigm of user-enriched content in the domain of sciences, with community-based approaches to scientific publishing. INSPIRE represents a natural evolution of scholarly communication built on successful community-based information systems, and it provides a vision for information management in other fields of science. Inspired by the needs of HEP, we hope that the INSPIRE project will be inspiring for other communities.

  20. HERUS: A CO ATLAS FROM SPIRE SPECTROSCOPY OF LOCAL ULIRGs

    Energy Technology Data Exchange (ETDEWEB)

    Pearson, Chris; Rigopoulou, Dimitra [RAL Space, CCLRC Rutherford Appleton Laboratory, Chilton, Didcot, Oxfordshire OX11 0QX (United Kingdom); Hurley, Peter [Department of Physics and Astronomy, University of Sussex, Falmer, Brighton BN1 9QH (United Kingdom); Farrah, Duncan [Department of Physics, Virginia Tech, Blacksburg, VA 24061 (United States); Afonso, Jose [Instituto de Astrofísica e Ciências do Espaç co, Universidade de Lisboa, OAL, Tapada da Ajuda, PT1349-018 Lisboa (Portugal); Bernard-Salas, Jeronimo [The Open University, MK7 6, AA Milton Keynes (United Kingdom); Borys, Colin [Riot Games, 12333 W Olympic Blvd, Los Angeles, CA 90064 (United States); Clements, David L. [Astrophysics Group, Imperial College London, Blackett Laboratory, Prince Consort Rd, London SW7 2AZ (United Kingdom); Cormier, Diane [Institut fur theoretische Astrophysik, Zentrum fur Astronomie der Universitat Heidelberg, Albert-Ueberle Str. 2, D-69120 Heidelberg (Germany); Efstathiou, Andreas [School of Sciences, European University Cyprus, Diogenes Street, Engomi, 1516 Nicosia (Cyprus); Gonzalez-Alfonso, Eduardo [Universidad de Alcala de Henares, Departamento de Fisica, Campus Universitario, E-28871 Alcala de Henares, Madrid (Spain); Lebouteiller, Vianney [CEA-Saclay, DSM/IRFU/SAp, F-91191 Gif-sur-Yvette (France); Spoon, Henrik [Cornell University, CRSR, Space Sciences Building, Ithaca, NY 14853 (United States)

    2016-11-01

    We present the Herschel SPIRE Fourier Transform Spectroscopy (FTS) atlas for a complete flux-limited sample of local ultraluminous infrared galaxies (ULIRGs) as part of the HERschel Ultra Luminous InfraRed Galaxy Survey (HERUS). The data reduction is described in detail and was optimized for faint FTS sources ,with particular care being taken for the subtraction of the background, which dominates the continuum shape of the spectra. To improve the final spectra, special treatment in the data reduction has been given to any observation suffering from artifacts in the data caused by anomalous instrumental effects. Complete spectra are shown covering 200–671 μ m, with photometry in the SPIRE bands at 250, 350, and 500 μ m. The spectra include near complete CO ladders for over half of our sample, as well as fine structure lines from [C i] 370 μ m, [C i] 609 μ m, and [N ii] 205 μ m. We also detect H{sub 2}O lines in several objects. We construct CO spectral line energy distributions (SLEDs) for the sample, and compare their slopes with the far-infrared (FIR) colors and luminosities. We show that the CO SLEDs of ULIRGs can be broadly grouped into three classes based on their excitation. We find that the mid- J (5 <  J  < 8) lines are better correlated with the total FIR luminosity, suggesting that the warm gas component is closely linked to recent star formation. The higher J transitions do not linearly correlate with the FIR luminosity, consistent with them originating in hotter, denser gas that is unconnected to the current star formation. We conclude that in most cases more than one temperature component is required to model the CO SLEDs.

  1. HERUS: A CO ATLAS FROM SPIRE SPECTROSCOPY OF LOCAL ULIRGs

    International Nuclear Information System (INIS)

    Pearson, Chris; Rigopoulou, Dimitra; Hurley, Peter; Farrah, Duncan; Afonso, Jose; Bernard-Salas, Jeronimo; Borys, Colin; Clements, David L.; Cormier, Diane; Efstathiou, Andreas; Gonzalez-Alfonso, Eduardo; Lebouteiller, Vianney; Spoon, Henrik

    2016-01-01

    We present the Herschel SPIRE Fourier Transform Spectroscopy (FTS) atlas for a complete flux-limited sample of local ultraluminous infrared galaxies (ULIRGs) as part of the HERschel Ultra Luminous InfraRed Galaxy Survey (HERUS). The data reduction is described in detail and was optimized for faint FTS sources ,with particular care being taken for the subtraction of the background, which dominates the continuum shape of the spectra. To improve the final spectra, special treatment in the data reduction has been given to any observation suffering from artifacts in the data caused by anomalous instrumental effects. Complete spectra are shown covering 200–671 μ m, with photometry in the SPIRE bands at 250, 350, and 500 μ m. The spectra include near complete CO ladders for over half of our sample, as well as fine structure lines from [C i] 370 μ m, [C i] 609 μ m, and [N ii] 205 μ m. We also detect H 2 O lines in several objects. We construct CO spectral line energy distributions (SLEDs) for the sample, and compare their slopes with the far-infrared (FIR) colors and luminosities. We show that the CO SLEDs of ULIRGs can be broadly grouped into three classes based on their excitation. We find that the mid- J (5 <  J  < 8) lines are better correlated with the total FIR luminosity, suggesting that the warm gas component is closely linked to recent star formation. The higher J transitions do not linearly correlate with the FIR luminosity, consistent with them originating in hotter, denser gas that is unconnected to the current star formation. We conclude that in most cases more than one temperature component is required to model the CO SLEDs.

  2. A mitochondria-anchored isoform of the actin-nucleating spire protein regulates mitochondrial division

    Science.gov (United States)

    Manor, Uri; Bartholomew, Sadie; Golani, Gonen; Christenson, Eric; Kozlov, Michael; Higgs, Henry; Spudich, James; Lippincott-Schwartz, Jennifer

    2015-01-01

    Mitochondrial division, essential for survival in mammals, is enhanced by an inter-organellar process involving ER tubules encircling and constricting mitochondria. The force for constriction is thought to involve actin polymerization by the ER-anchored isoform of the formin protein inverted formin 2 (INF2). Unknown is the mechanism triggering INF2-mediated actin polymerization at ER-mitochondria intersections. We show that a novel isoform of the formin-binding, actin-nucleating protein Spire, Spire1C, localizes to mitochondria and directly links mitochondria to the actin cytoskeleton and the ER. Spire1C binds INF2 and promotes actin assembly on mitochondrial surfaces. Disrupting either Spire1C actin- or formin-binding activities reduces mitochondrial constriction and division. We propose Spire1C cooperates with INF2 to regulate actin assembly at ER-mitochondrial contacts. Simulations support this model's feasibility and demonstrate polymerizing actin filaments can induce mitochondrial constriction. Thus, Spire1C is optimally positioned to serve as a molecular hub that links mitochondria to actin and the ER for regulation of mitochondrial division. DOI: http://dx.doi.org/10.7554/eLife.08828.001 PMID:26305500

  3. INSPIRE: a new scientific information system for HEP

    CERN Multimedia

    Ivanov, R

    2009-01-01

    The status of high-energy physics (HEP) information systems has been jointly analyzed by the libraries of CERN, DESY, Fermilab and SLAC. As a result, the four laboratories have started the INSPIRE project – a new platform built by moving the successful SPIRES features and content, curated at DESY, Fermilab and SLAC, into the open-source CDS Invenio digital library software that was developed at CERN. INSPIRE will integrate present acquisition workflows and databases to host the entire body of the HEP literature (about one million records), aiming to become the reference HEP scientific information platform worldwide. It will provide users with fast access to full-text journal articles and preprints, but also material such as conference slides and multimedia. INSPIRE will empower scientists with new tools to discover and access the results most relevant to their research, enable novel text- and data-mining applications, and deploy new metrics to assess the impact of articles and authors. In addition, it will ...

  4. Effective delivery of hydrophobic drugs to breast (MCF-7) and Liver (HepG2) cancer cells: A detailed investigation using Cytotoxicity assays, fluorescence imaging and flow cytometry.

    Science.gov (United States)

    Manatunga, Danushika C; de Silva, Rohini M; Nalin de Silva, K M; Neelika Malavige, Gathsaurie; Wijeratne, Dulharie T; Williams, Gareth R; Jayasinghe, Chanika D; Udagama, Preethi V

    2018-04-03

    This study aimed to develop a drug carrier system consisting of a polymer containing hydroxyapatite (HAp) shell and a magnetic core of iron oxide nanoparticles. Doxorubicin and/or curcumin were loaded into the carrier via a simple diffusion deposition approach, with encapsulation efficiencies (EE) for curcumin and doxorubicin of 93.03 ± 0.3% and 97.37 ± 0.12% respectively. The co-loading of curcumin and doxorubicin led to a total EE of 76.02 ± 0.48%. Release studies were carried out at pH 7.4 and 5.3, and revealed higher release was at pH 5.3 expressing the potential application in tumor microenvironments. Cytotoxicity assays, fluorescence imaging and flow cytometry showed the formulations could effectively inhibit the growth of MCF-7 and HEpG2 cancer cells, being more potent than the free drug molecules both in dose and time dependent manner. Additionally, hemolysis tests and cytotoxicity evaluations determined the drug-loaded carriers to be non-toxic towards non-cancerous cells. These formulations thus have great potential in the development of new cancer therapeutics. Copyright © 2018. Published by Elsevier B.V.

  5. Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells.

    Science.gov (United States)

    König, Alexander; Glebe, Dieter

    2017-01-01

    To obtain basic knowledge about specific molecular mechanisms involved in the entry of pathogens into cells is the basis for establishing pharmacologic substances blocking initial viral binding, infection, and subsequent viral spread. Lack of information about key cellular factors involved in the initial steps of HBV infection has hampered the characterization of HBV binding and entry for decades. However, recently, the liver-specific sodium-dependent taurocholate cotransporting polypeptide (NTCP) has been discovered as a functional receptor for HBV and HDV, thus opening the field for new concepts of basic binding and entry of HBV and HDV. Here, we describe practical issues of a basic in vitro assay system to examine kinetics and mechanisms of receptor-dependent HBV binding, uptake, and intracellular trafficking by live-cell imaging confocal microscopy. The assay system is comprised of HepG2 cells expressing a NTCP-GFP fusion-protein and chemically synthesized, fluorophore-labeled part of HBV surface protein, spanning the first N-terminal 48 amino acids of preS1 of the large hepatitis B virus surface protein.

  6. Herschel SPIRE FTS spectral line source calibrators

    DEFF Research Database (Denmark)

    Hopwood, Rosalind; Polehampton, Edward; Valtchanov, Ivan

    2015-01-01

    We present a summary of the Herschel SPIRE/FTS calibration programme to monitor the repeatability of spectral lines. Observations of planetary nebulae and post-AGB stars are used to assess repeatability and model the asymmetry of the instrument line shape.......We present a summary of the Herschel SPIRE/FTS calibration programme to monitor the repeatability of spectral lines. Observations of planetary nebulae and post-AGB stars are used to assess repeatability and model the asymmetry of the instrument line shape....

  7. SPIRES Tailored to a Special Library: A Mainframe Answer for a Small Online Catalog.

    Science.gov (United States)

    Newton, Mary

    1989-01-01

    Describes the design and functions of a technical library database maintained on a mainframe computer and supported by the SPIRES database management system. The topics covered include record structures, vocabulary control, input procedures, searching features, time considerations, and cost effectiveness. (three references) (CLB)

  8. Spire and Formin 2 synergize and antagonize in regulating actin assembly in meiosis by a ping-pong mechanism.

    Directory of Open Access Journals (Sweden)

    Pierre Montaville

    2014-02-01

    Full Text Available In mammalian oocytes, three actin binding proteins, Formin 2 (Fmn2, Spire, and profilin, synergistically organize a dynamic cytoplasmic actin meshwork that mediates translocation of the spindle toward the cortex and is required for successful fertilization. Here we characterize Fmn2 and elucidate the molecular mechanism for this synergy, using bulk solution and individual filament kinetic measurements of actin assembly dynamics. We show that by capping filament barbed ends, Spire recruits Fmn2 and facilitates its association with barbed ends, followed by rapid processive assembly and release of Spire. In the presence of actin, profilin, Spire, and Fmn2, filaments display alternating phases of rapid processive assembly and arrested growth, driven by a "ping-pong" mechanism, in which Spire and Fmn2 alternately kick off each other from the barbed ends. The results are validated by the effects of injection of Spire, Fmn2, and their interacting moieties in mouse oocytes. This original mechanism of regulation of a Rho-GTPase-independent formin, recruited by Spire at Rab11a-positive vesicles, supports a model for modulation of a dynamic actin-vesicle meshwork in the oocyte at the origin of asymmetric positioning of the meiotic spindle.

  9. Method of compensation spires for the detection of the diamagnetic effect in a Tokamak

    International Nuclear Information System (INIS)

    Colunga S, S.

    1990-09-01

    In this report the classical detection method of the diamagnetic effect by means of a rolled spire on the discharges chamber in the poloidal direction and the difficulties related with this are analyzed. An alternative method that increases considerably the detection sensibility of the diamagnetic effect and that for its simplicity it is quite attractive for its application to the Tokamak Novillo of the ININ is presented. (Author)

  10. Structure and function of the interacting domains of Spire and Fmn-family formins

    Energy Technology Data Exchange (ETDEWEB)

    Vizcarra, Christina L.; Kreutz, Barry; Rodal, Avital A.; Toms, Angela V.; Lu, Jun; Zheng, Wei; Quinlan, Margot E.; Eck, Michael J. (UCLA); (Brandeis); (DFCI)

    2012-07-11

    Evidence for cooperation between actin nucleators is growing. The WH2-containing nucleator Spire and the formin Cappuccino interact directly, and both are essential for assembly of an actin mesh during Drosophila oogenesis. Their interaction requires the kinase noncatalytic C-lobe domain (KIND) domain of Spire and the C-terminal tail of the formin. Here we describe the crystal structure of the KIND domain of human Spir1 alone and in complex with the tail of Fmn2, a mammalian ortholog of Cappuccino. The KIND domain is structurally similar to the C-lobe of protein kinases. The Fmn2 tail is coordinated in an acidic cleft at the base of the domain that appears to have evolved via deletion of a helix from the canonical kinase fold. Our functional analysis of Cappuccino reveals an unexpected requirement for its tail in actin assembly. In addition, we find that the KIND/tail interaction blocks nucleation by Cappuccino and promotes its displacement from filament barbed ends providing insight into possible modes of cooperation between Spire and Cappuccino.

  11. Structure and function of the interacting domains of Spire and Fmn-family formins

    Science.gov (United States)

    Vizcarra, Christina L.; Kreutz, Barry; Rodal, Avital A.; Toms, Angela V.; Lu, Jun; Zheng, Wei; Quinlan, Margot E.; Eck, Michael J.

    2011-01-01

    Evidence for cooperation between actin nucleators is growing. The WH2-containing nucleator Spire and the formin Cappuccino interact directly, and both are essential for assembly of an actin mesh during Drosophila oogenesis. Their interaction requires the kinase noncatalytic C-lobe domain (KIND) domain of Spire and the C-terminal tail of the formin. Here we describe the crystal structure of the KIND domain of human Spir1 alone and in complex with the tail of Fmn2, a mammalian ortholog of Cappuccino. The KIND domain is structurally similar to the C-lobe of protein kinases. The Fmn2 tail is coordinated in an acidic cleft at the base of the domain that appears to have evolved via deletion of a helix from the canonical kinase fold. Our functional analysis of Cappuccino reveals an unexpected requirement for its tail in actin assembly. In addition, we find that the KIND/tail interaction blocks nucleation by Cappuccino and promotes its displacement from filament barbed ends providing insight into possible modes of cooperation between Spire and Cappuccino. PMID:21730168

  12. Flux calibration of the Herschel(star)-SPIRE photometer

    NARCIS (Netherlands)

    Bendo, G. J.; Griffin, M. J.; Bock, J. J.; Conversi, L.; Dowell, C. D.; Lim, T.; Lu, N.; North, C. E.; Papageorgiou, A.; Pearson, C. P.; Pohlen, M.; Polehampton, E. T.; Schulz, B.; Shupe, D. L.; Sibthorpe, B.; Spencer, L. D.; Swinyard, B. M.; Valtchanov, I.; Xu, C. K.

    We describe the procedure used to flux calibrate the three-band submillimetre photometer in the Spectral and Photometric Imaging Receiver instrument on the Herschel Space Observatory. This includes the equations describing the calibration scheme, a justification for using Neptune as the primary

  13. HEP C++ meets reality

    Energy Technology Data Exchange (ETDEWEB)

    Eulisse, Giulio; Tuura, Lassi; Elmer, Peter

    2010-04-01

    In 2007 the CMS experiment first reported some initial findings on the impedance mismatch between HEP use of C++ and the current generation of compilers and CPUs. Since then we have continued our analysis of the CMS experiment code base, including the external packages we use. We have found that large amounts of C++ code has been written largely ignoring any physical reality of the resulting machine code and run time execution costs, including and especially software developed by experts. We report on a wide range issues affecting typical high energy physics code, in the form of coding pattern - impact - lesson - improvement.

  14. HEP C++ meets reality

    International Nuclear Information System (INIS)

    Eulisse, Giulio; Tuura, Lassi; Elmer, Peter

    2010-01-01

    In 2007 the CMS experiment first reported some initial findings on the impedance mismatch between HEP use of C++ and the current generation of compilers and CPUs. Since then we have continued our analysis of the CMS experiment code base, including the external packages we use. We have found that large amounts of C++ code has been written largely ignoring any physical reality of the resulting machine code and run time execution costs, including and especially software developed by experts. We report on a wide range issues affecting typical high energy physics code, in the form of coding pattern - impact - lesson - improvement.

  15. HEP C++ Meets reality

    CERN Document Server

    Eulisse, Giulio; Elmer, Peter

    2009-01-01

    In 2007 the CMS experiment first reported some initial findings on the impedance mismatch between HEP use of C++ and the current generation of compilers and CPUs. Since then we have continued our analysis of the CMS experiment code base, including the external packages we use. We have found that large amounts of C++ code has been written largely ignoring any physical reality of the resulting machine code and run time execution costs, including and especially software developed by experts. We report on a wide range issues affecting typical high energy physics code, in the form of coding pattern - impact - lesson - improvement.

  16. HEP data analysis using jHepWork and Java

    International Nuclear Information System (INIS)

    Chekanov, S.

    2009-01-01

    A role of Java in high-energy physics (HEP) and recent progress in development of a platform-independent data-analysis framework, jHepWork, is discussed. The framework produces professional graphics and has many libraries for data manipulation.

  17. Esteatose hepática na obesidade infantil: investigação por imagem = Hepatic steatosis in obese children: imaging investigation

    Directory of Open Access Journals (Sweden)

    Soder, Ricardo Bernardi

    2009-01-01

    Conclusões: dentre as vantagens e desvantagens próprias de cada método de imagem, a ressonância magnética é considerada a melhor modalidade no diagnóstico e quantificação da infiltração gordurosa hepática. No entanto, a investigação por imagem poderá ser iniciada com um método menos sofisticado, como a ultrassonografia, que é uma alternativa mais acessível e econômica

  18. The Herschel Multi-Tiered Extragalactic Survey: SPIRE-mm Photometric Redshifts

    Science.gov (United States)

    Roseboom, I. G.; Ivison, R. J.; Greve, T. R.; Amblard, A.; Arumugam, V.; Auld, R.; Aussel, H.; Bethermin, M.; Blain, A.; Bock, J.; hide

    2011-01-01

    We investigate the potential of submm-mm and submm-mm-radio photometric red-shifts using a sample of mm-selected sources as seen at 250, 350 and 500 micrometers by the SPIRE instrument on Herschel. From a sample of 63 previously identified mm-sources with reliable radio identifications in the GOODS-N and Lockman Hole North fields 46 (73 per cent) are found to have detections in at least one SPIRE band. We explore the observed submm/mm colour evolution with redshift, finding that the colours of mm-sources are adequately described by a modified blackbody with constant optical depth Tau = (nu/nu(0))beta where beta = +1.8 and nu(0) = c/100 micrometers. We find a tight correlation between dust temperature and IR luminosity. Using a single model of the dust temperature and IR luminosity relation we derive photometric redshift estimates for the 46 SPIRE detected mm-sources. Testing against the 22 sources with known spectroscopic, or good quality optical/near-IR photometric, redshifts we find submm/mm photometric redshifts offer a redshift accuracy of |delta z|/(1+z) = 0.16 (less than |delta z| greater than = 0.51). Including constraints from the radio-far IR correlation the accuracy is improved to |delta z|/(1 + z) = 0.15 (less than |delta z| greater than = 0.45). We estimate the redshift distribution of mm-selected sources finding a significant excess at z greater than 3 when compared to 850 micrometer selected samples.

  19. Probing the molecular interstellar medium of M82 with Herschel-SPIRE spectroscopy

    OpenAIRE

    Panuzzo, P.; Rangwala, N.; Rykala, Adam John; Isaak, Kate Gudrun; Glenn, J.; Wilson, C. D.; Auld, Robbie Richard; Baes, M.; Barlow, M. J.; Bendo, G. J.; Bock, J. J.; Boselli, A.; Bradford, M.; Buat, V.; Castro-Rodríguez, N.

    2010-01-01

    We present the observations of the starburst galaxy M82 taken with the Herschel SPIRE Fourier-transform spectrometer. The spectrum (194-671 mu m) shows a prominent CO rotational ladder from J = 4-3 to 13-12 emitted by the central region of M82. The fundamental properties of the gas are well constrained by the high J lines observed for the first time. Radiative transfer modeling of these high-S/N (CO)-C-12 and (CO)-C-13 lines strongly indicates a very warm molecular gas component at similar to...

  20. License - DB-SPIRE | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us DB-SPIRE License License to Use This Database Last updated : 2017/02/16 You may use this database...license terms regarding the use of this database and the requirements you must follow in using this database...Share Alike 4.0 International . If you use data from this database, please be sure attribute this database a...eative Commons Attribution-Share Alike 4.0 International is found here . With regard to this database, you a...re licensed to: freely access part or whole of this database, and acquire data; freely redistribute part or

  1. GNSS Radio Occultation Methods for CubeSat Missions: The University of Calgary and Spire Partnership

    Science.gov (United States)

    Skone, S.; Swab, M.; Platzer, P.; Johl, S.; Cappaert, J.

    2014-12-01

    In 2008, the University of Calgary deployed a low-cost commercial-off-the-shelf dual frequency GPS receiver onboard the CanX-2 nanosatellite, with the goal of demonstrating single-antenna single-receiver GNSS radio occultation capabilities. The team successfully produced ionospheric electron density profiles and continues to operate the CanX-2 GPS payload and collect reliable data six years into the mission. Recently the University of Calgary partnered with Spire to develop low-cost atmospheric sounding methods based on GNSS radio occultations for nanosatellite platforms. The rapidly increasing capabilities on nanosatellites with regards to power production, pointing accuracy and antenna sensitivities provide an ever more attractive platform to create relevant solutions for space and terrestrial weather data. This paper describes future mission concepts and capabilities for multi-GNSS methods to generate high-resolution atmospheric profiles. Building on lessons learned from CanX-2, the system requirements are defined and recommendations made for efficient GNSS payload operations. New methods are assessed for multi-frequency multi-constellation GNSS radio occultation approaches. Software and hardware simulations are conducted for validation of proposed methods using appropriate receiver architectures. Analyses include signal tracking for LEO trajectories (and Dopplers) and high-precision navigation solutions. Initial data analysis is also presented for a miniaturized, multi-frequency, software-­defined GNSS receiver currently operating onboard Spire's innovative CubeSat platform.

  2. Ressonância magnética hepática em puérperas estáveis com síndrome HELLP Magnetic resonance imaging of the liver in postpartum stable women with HELLP syndrome

    Directory of Open Access Journals (Sweden)

    Ana Rita Marinho Ribeiro Carvalho

    2008-10-01

    Full Text Available OBJETIVOS: Descrever os achados hepáticos na ressonância magnética em puérperas estáveis com síndrome HELLP. MÉTODOS: Realizou-se um estudo descritivo, do tipo série de casos, envolvendo 40 puérperas internadas na UTI obstétrica do Instituto Materno Infantil Prof. Fernando Figueira (IMIP, com diagnóstico de síndrome HELLP completa (presentes todas as alterações laboratoriais e incompleta (uma ou mais alterações laboratoriais, porém sem todos os critérios diagnósticos no período de agosto de 2005 a julho de 2006. RESULTADOS: A idade média foi de 26,8 ± 6,4 anos, com idade gestacional média no parto de 34 semanas. A ressonância magnética foi realizada entre oito e 96 horas depois do diagnóstico de síndrome HELLP (média de 56 + 31horas. O achado mais freqüente foi ascite em 20% (n = 8, seguindo-se derrame pleural (17,5% e esteatose hepática (7,5%. A intensidade de sinal periportal foi normal em todos os casos e não se observaram casos de isquemia/infarto hepático ou de hematoma parenquimatoso ou subcapsular. CONCLUSÃO: Os achados da ressonância magnética pós-parto em puérperas estáveis com síndrome HELLP foram inespecíficos e, na presente série, não foram encontradas lesões importantes como hematoma parenquimatoso ou subcapsular, representando risco de vida para a paciente. Os resultados encontrados não corroboram a utilização desse exame de rotina para o seguimento de pacientes com síndrome HELLP.OBJECTIVES: To describe magnetic resonance (MR findings in the liver of stable patients with HELLP syndrome in the puerpuerium. METHODS: A descriptive study was carried out from August 2005 to July 2006, involving a series of 40 postpartum patients admitted to an obstetric intensive therapy unit in IMIP (Instituto Materno Infantil Prof. Fernando Figueira with diagnosis of HELLP syndrome (complete and partial. Complete HELLP syndrome was defined when all laboratory parameters were present and incomplete when

  3. Databases and bookkeeping for HEP experiments

    International Nuclear Information System (INIS)

    Blobel, V.; Cnops, A.-M.; Fisher, S.M.

    1983-09-01

    The term database is explained as well as the requirements for data bases in High Energy physics (HEP). Also covered are the packages used in HEP, summary of user experience, database management systems, relational database management systems for HEP use and observations. (U.K.)

  4. Networking: the view from HEP

    Science.gov (United States)

    McKee, Shawn

    2017-10-01

    Networks have played a critical role in high-energy physics (HEP), enabling us to access and effectively utilize globally distributed resources to meet the needs of our physicists. National and global-scale collaborations that characterize HEP would not be feasible without ubiquitous capable networks. Because of their importance in enabling our grid computing infrastructure many physicists have taken leading roles in research and education (R&E) networking, participating in, and even convening, network related meetings and research programs with the broader networking community worldwide. This has led to HEP benefiting from excellent global networking capabilities for little to no direct cost. However, as other science domains ramp-up their need for similar networking it becomes less clear that this situation will continue unchanged. This paper will briefly discuss the history of networking in HEP, the current activities and challenges we are facing, and try to provide some understanding of where networking may be going in the next 5 to 10 years.

  5. LEP dominates LP-HEP

    International Nuclear Information System (INIS)

    Fraser, Gordon

    1991-01-01

    CERN's LEP electron-positron collider was the star of this year's major physics meeting - the Joint International Lepton-Photon Symposium and Europhysics Conference on High Energy Physics (LP-HEP) - held in Geneva from 25 July - 1 August

  6. HEP visualization and video technology

    International Nuclear Information System (INIS)

    Lebrun, P.; Swoboda, D.

    1994-01-01

    The use of scientific visualization for HEP analysis is briefly reviewed. The applications are highly interactive and very dynamical in nature. At Fermilab, E687, in collaboration with Visual Media Services, has produced a 1/2 hour video tape demonstrating the capability of SGI-EXPLORER applied to a Dalitz Analysis of Charm decay. This short contribution describes the authors experience with visualization and video technologies

  7. New opportunities for allergen immunotherapy using synthetic peptide immuno-regulatory epitopes (SPIREs).

    Science.gov (United States)

    Klimek, Ludger; Pfaar, Oliver; Worm, Margitta

    2016-10-01

    Allergen immunotherapy (AIT) reduces allergic rhinoconjunctivitis (ARC) symptoms, but long-term efficacy requires treatment for 3-5 years. Synthetic peptide immuno-regulatory epitopes, a new class of AIT, are allergen peptides with a shorter, more convenient treatment regimen that could potentially have benefits on adherence and outcomes. Phase 2 trials of therapies derived from cat, house dust mite, grass, and ragweed allergen peptides demonstrated significant reduction in ARC symptoms after short-course treatment; improvement was sustained for 18-24 months posttreatment. We conducted a PubMed literature search for clinical publications using the search terms AIT; allergen peptides; ARC; cat, grass, house dust mite, and ragweed allergy; SCIT; SLIT; and synthetic peptides. Expert commentary: Long-term disease modification is a realistic goal of AIT. The inconvenience of conventional AIT regimens negatively impacts long-term persistence and, thus, efficacy. In comparison, SPIREs have a more convenient treatment regimen that could potentially have benefits on adherence and outcomes.

  8. Dendrite architecture organized by transcriptional control of the F-actin nucleator Spire.

    Science.gov (United States)

    Ferreira, Tiago; Ou, Yimiao; Li, Sally; Giniger, Edward; van Meyel, Donald J

    2014-02-01

    The architectures of dendritic trees are crucial for the wiring and function of neuronal circuits because they determine coverage of receptive territories, as well as the nature and strength of sensory or synaptic inputs. Here, we describe a cell-intrinsic pathway sculpting dendritic arborization (da) neurons in Drosophila that requires Longitudinals Lacking (Lola), a BTB/POZ transcription factor, and its control of the F-actin cytoskeleton through Spire (Spir), an actin nucleation protein. Loss of Lola from da neurons reduced the overall length of dendritic arbors, increased the expression of Spir, and produced inappropriate F-actin-rich dendrites at positions too near the cell soma. Selective removal of Lola from only class IV da neurons decreased the evasive responses of larvae to nociception. The increased Spir expression contributed to the abnormal F-actin-rich dendrites and the decreased nocifensive responses because both were suppressed by reduced dose of Spir. Thus, an important role of Lola is to limit expression of Spir to appropriate levels within da neurons. We found Spir to be expressed in dendritic arbors and to be important for their development. Removal of Spir from class IV da neurons reduced F-actin levels and total branch number, shifted the position of greatest branch density away from the cell soma, and compromised nocifensive behavior. We conclude that the Lola-Spir pathway is crucial for the spatial arrangement of branches within dendritic trees and for neural circuit function because it provides balanced control of the F-actin cytoskeleton.

  9. A reflection on Software Engineering in HEP

    International Nuclear Information System (INIS)

    Carminati, Federico

    2012-01-01

    High Energy Physics (HEP) has been making very extensive usage of computers to achieve its research goals. Fairly large program suites have been developed, maintained and used over the years and it is fair to say that, overall, HEP has been successful in software development. Yet, HEP software development has not used classical Software Engineering techniques, which have been invented and refined to help the production of large programmes. In this paper we will review the development of HEP code with its strengths and weaknesses. Using several well-known HEP software projects as examples, we will try to demonstrate that our community has used a form of Software Engineering, albeit in an informal manner. The software development techniques employed in these projects are indeed very close in many aspects to the modern tendencies of Software Engineering itself, in particular the so-called “agile technologies”. The paper will conclude with an outlook on the future of software development in HEP.

  10. As imagens não falam por si mesmas: um estudo sociológico da equipe do transplante hepático Images do not speak for themselves: a sociological study of a liver transplantation team

    Directory of Open Access Journals (Sweden)

    Sandra Mara Maciel-Lima

    2012-03-01

    Full Text Available O presente artigo visa entender como uma equipe de transplante hepático se relaciona com a constante tensão entre certeza e incerteza nas práticas médicas associadas à utilização de imagens durante o processo de transplante. Para tanto, utiliza-se a metodologia de abordagem qualitativa, a etnografia e o estudo de caso como procedimentos técnicos. Os dados foram coletados por meio de observação e entrevistas semidiretivas realizadas com a equipe de transplante do Hospital de Clínicas da UFPR. Toda imagem demanda a análise e interpretação, de preferência de um especialista em imagem, capaz de identificar o que esta revela. E é nesse momento que se percebem as insuficiências das imagens e ao mesmo tempo do manifesto pela certeza; da ilusão de uma prática de interpretação sem a presença da subjetividade de quem interpreta. Na análise de imagens médicas o que está em jogo também é o modo de ver o objeto analisado. Muitas vezes o que se vê são fragmentos do que ocorre no corpo, e a interpretação dessa imagem pressupõe informações que não estão visíveis, mas que são capturadas pela experiência e pelo conhecimento adquiridos pelo médico ao longo do tempo e também na sua relação com o paciente. Percebe-se, assim, que mesmo com o desenvolvimento das tecnologias de imagem no campo médico, não se elimina a tensão entre a objetividade e a subjetividade, entre a certeza e a incerteza, entre o saber e o ver.This article aims to understand how a liver transplantation team deals with the constant tension between certainty and uncertainty in medical practices associated with the use of images in the transplantation process. We used the methodology of qualitative approach, ethnography and case study as technical procedures. Data were collected through observation and through semi-directive interviews performed with the transplantation team of Hospital das Clínicas of UFPR. Every image requires analysis and

  11. Data synchronisation for HEP experiments

    Science.gov (United States)

    Byszuk, Adrian

    2017-08-01

    One of the most important tasks handled by electronic systems in High Energy Physics (HEP) experiments is data acquisition (DAQ). Architecture and requirements of DAQ systems are tightly coupled. DAQ requirements strongly influence system architecture and, at the same time, inherent constraints of chosen architecture (and hardware) may necessitate update of DAQ system requirements. One of the most important characteristics of a given DAQ system is it's data synchronisation capability, which is constrained by performance of experiment's timing system. This paper presents an overview of most popular timing system architectures and describes how this choice influences data synchronisation capabilities.

  12. Benchmarking Cloud Resources for HEP

    Science.gov (United States)

    Alef, M.; Cordeiro, C.; De Salvo, A.; Di Girolamo, A.; Field, L.; Giordano, D.; Guerri, M.; Schiavi, F. C.; Wiebalck, A.

    2017-10-01

    In a commercial cloud environment, exhaustive resource profiling is beneficial to cope with the intrinsic variability of the virtualised environment, allowing to promptly identify performance degradation. In the context of its commercial cloud initiatives, CERN has acquired extensive experience in benchmarking commercial cloud resources. Ultimately, this activity provides information on the actual delivered performance of invoiced resources. In this report we discuss the experience acquired and the results collected using several fast benchmark applications adopted by the HEP community. These benchmarks span from open-source benchmarks to specific user applications and synthetic benchmarks. The workflow put in place to collect and analyse performance metrics is also described.

  13. Batch calculations in CalcHEP

    International Nuclear Information System (INIS)

    Pukhov, A.

    2003-01-01

    CalcHEP is a clone of the CompHEP project which is developed by the author outside of the CompHEP group. CompHEP/CalcHEP are packages for automatic calculations of elementary particle decay and collision properties in the lowest order of perturbation theory. The main idea prescribed into the packages is to make available passing on from the Lagrangian to the final distributions effectively with a high level of automation. According to this, the packages were created as a menu driven user friendly programs for calculations in the interactive mode. From the other side, long-time calculations should be done in the non-interactive regime. Thus, from the beginning CompHEP has a problem of batch calculations. In CompHEP 33.23 the batch session was realized by mean of interactive menu which allows to the user to formulate the task for batch. After that the not-interactive session was launched. This way is too restricted, not flexible, and leads to doubling in programming. In this article I discuss another approach how one can force an interactive program to work in non-interactive mode. This approach was realized in CalcHEP 2.1 disposed on http://theory.sinp.msu.ru/~pukhov/calchep.html

  14. Warm and cold molecular gas conditions modeled in 87 galaxies observed by the Herschel SPIRE FTS

    Science.gov (United States)

    Kamenetzky, Julia; Rangwala, Naseem; Glenn, Jason

    2018-01-01

    Molecular gas is the raw material for star formation, and like the interstellar medium (ISM) in general, it can exist in regions of higher and lower excitation. Rotational transitions of the CO molecule are bright and sensitive to cold molecular gas. While the majority of the molecular gas exists in the very cold component traced by CO J=1-0, the higher-J lines trace the highly excited gas that may be more indicative of star formation processes. The atmosphere is opaque to these lines, but the launch of the Herschel Space Observatory made them accessible for study of Galactic and extragalactic sources. We have conducted two-component, non-local thermodynamic equilibrium (non-LTE) modeling of the CO lines from J=1‑0 through J=13‑12 in 87 galaxies observed by the Herschel SPIRE Fourier Transform Spectrometer (FTS). We used the nested sampling algorithm Multinest to compare the measured CO spectral line energy distributions (SLEDs) to the ones produced by a custom version of the non-LTE code RADEX. This allowed us to fully examine the degeneracies in parameter space for kinetic temperature, molecular gas density, CO column density, and area filling factor.Here we discuss the major findings of our study, as well as the important implications of two-component molecular gas modeling. The average pressure of the warm gas is slightly correlated with galaxy LFIR, but that of the cold gas is not. A high-J (such as J=11-10) to J=1-0 line ratio is diagnostic of warm component pressure. We find a very large spread in our derived values of "alpha-CO," with no discernable trend with LFIR, and average molecular gas depletion times that decrease with LFIR. If only a few molecular lines are available in a galaxy's SLED, the limited ability to model only one component will change the results. A one-component fit often underestimates the flux of carbon monoxide (CO) J=1‑0 and the mass. If low-J lines are not included, mass is underestimated by an order of magnitude. Even when

  15. THE CDF ARCHIVE: HERSCHEL PACS AND SPIRE SPECTROSCOPIC DATA PIPELINE AND PRODUCTS FOR PROTOSTARS AND YOUNG STELLAR OBJECTS

    Energy Technology Data Exchange (ETDEWEB)

    Green, Joel D.; Yang, Yao-Lun; II, Neal J. Evans; Larson, Rebecca L. [The University of Texas at Austin, Department of Astronomy, 2515 Speedway, Stop C1400, Austin, TX 78712-1205 (United States); Karska, Agata [Astronomical Observatory Institute, Faculty of Physics, A. Mickiewicz University, Sloneczna 36, 60-286 Poznan (Poland); Herczeg, Gregory [Kavli Institute for Astronomy and Astrophysics, Peking University, Yi He Yuan Lu 5, Haidian Qu, 100871 Beijing (China); Dishoeck, Ewine F. van [Leiden Observatory, Leiden University (Netherlands); Lee, Jeong-Eun [Department of Astronomy and Space Science, Kyung Hee University, Gyeonggi 446-701 (Korea, Republic of); Bouwman, Jeroen [Max Planck Institute for Astronomy, Heidelberg (Germany)

    2016-03-15

    We present the COPS-DIGIT-FOOSH (CDF) Herschel spectroscopy data product archive, and related ancillary data products, along with data fidelity assessments, and a user-created archive in collaboration with the Herschel-PACS and SPIRE ICC groups. Our products include datacubes, contour maps, automated line fitting results, and best 1D spectra products for all protostellar and disk sources observed with PACS in RangeScan mode for two observing programs: the DIGIT Open Time Key Program (KPOT-nevans-1 and SDP-nevans-1; PI: N. Evans), and the FOOSH Open Time Program (OT1-jgreen02-2; PI: J. Green). In addition, we provide our best SPIRE-FTS spectroscopic products for the COPS Open Time Program (OT2-jgreen02-6; PI: J. Green) and FOOSH sources. We include details of data processing, descriptions of output products, and tests of their reliability for user applications. We identify the parts of the data set to be used with caution. The resulting absolute flux calibration has improved in almost all cases. Compared to previous reductions, the resulting rotational temperatures and numbers of CO molecules have changed substantially in some sources. On average, however, the rotational temperatures have not changed substantially (<2%), but the number of warm (T{sub rot} ∼ 300 K) CO molecules has increased by about 18%.

  16. Analysis Traceability and Provenance for HEP

    Science.gov (United States)

    Shamdasani, Jetendr; McClatchey, Richard; Branson, Andrew; Kovács, Zsolt

    2015-12-01

    This paper presents the use of the CRISTAL software in the N4U project. CRISTAL was used to create a set of provenance aware analysis tools for the Neuroscience domain. This paper advocates that the approach taken in N4U to build the analysis suite is sufficiently generic to be able to be applied to the HEP domain. A mapping to the PROV model for provenance interoperability is also presented and how this can be applied to the HEP domain for the interoperability of HEP analyses.

  17. O valor da ressonância magnética na detecção de nódulos hepáticos utilizando-se diversas técnicas ponderadas em T2: existe consenso? The value of magnetic resonance imaging for the detection of focal liver lesions using different T-2 weighted techniques: is there a consensus?

    Directory of Open Access Journals (Sweden)

    Glaucia Andrade e Silva Palácio

    2002-11-01

    Full Text Available Desde o início da década de 80 a ressonância magnética vem sendo utilizada para o estudo do abdome e principalmente na detecção de nódulos hepáticos. As imagens ponderadas em T2 são as que trouxeram maior benefício quando comparadas à tomografia computadorizada com contraste. Inúmeras técnicas e seqüências de ressonância magnética ponderadas em T2 surgiram desde então, na tentativa de aumentar a eficácia diagnóstica, com menores tempos de exame. Neste sentido, foram publicados inúmeros trabalhos demonstrando a utilidade de seqüências rápidas e ultra-rápidas, com e sem supressão de gordura, em apnéia, com sincronizador respiratório e com bobinas de sinergia, entre outros avanços tecnológicos. No entanto, não há um consenso sobre qual a técnica mais apropriada e sensível para a detecção de lesões hepáticas focais. Neste artigo fazemos uma revisão bibliográfica e análise crítica das diversas técnicas de imagens ponderadas em T2, no que diz respeito às suas sensibilidades na detecção de nódulos hepáticos.Since the early 1980's several magnetic resonance imaging pulse sequences have been developed in order to determine the optimum imaging technique for the detection and characterization of hepatic lesions. T2-weighted images play an important role in the evaluation of the liver and present equal or greater sensitivity than enhanced computed tomography for the detection of liver lesions. New techniques for obtaining T-2 weighted images have been developed in the attempt to optimize the method. These techniques have improved the image quality by shortening examination time, reducing motion artifacts, and improving contrast-to-noise ratio. The effectiveness of the different techniques (fat suppression, breath-hold, respiratory-triggered and phased-array coils has been tested in many comparative studies, although the results are controversial. In this article we review the literature and discuss the several

  18. Hydrological Ensemble Prediction System (HEPS)

    Science.gov (United States)

    Thielen-Del Pozo, J.; Schaake, J.; Martin, E.; Pailleux, J.; Pappenberger, F.

    2010-09-01

    Flood forecasting systems form a key part of ‘preparedness' strategies for disastrous floods and provide hydrological services, civil protection authorities and the public with information of upcoming events. Provided the warning leadtime is sufficiently long, adequate preparatory actions can be taken to efficiently reduce the impacts of the flooding. Following on the success of the use of ensembles for weather forecasting, the hydrological community now moves increasingly towards Hydrological Ensemble Prediction Systems (HEPS) for improved flood forecasting using operationally available NWP products as inputs. However, these products are often generated on relatively coarse scales compared to hydrologically relevant basin units and suffer systematic biases that may have considerable impact when passed through the non-linear hydrological filters. Therefore, a better understanding on how best to produce, communicate and use hydrologic ensemble forecasts in hydrological short-, medium- und long term prediction of hydrological processes is necessary. The "Hydrologic Ensemble Prediction Experiment" (HEPEX), is an international initiative consisting of hydrologists, meteorologist and end-users to advance probabilistic hydrologic forecast techniques for flood, drought and water management applications. Different aspects of the hydrological ensemble processor are being addressed including • Production of useful meteorological products relevant for hydrological applications, ranging from nowcasting products to seasonal forecasts. The importance of hindcasts that are consistent with the operational weather forecasts will be discussed to support bias correction and downscaling, statistically meaningful verification of HEPS, and the development and testing of operating rules; • Need for downscaling and post-processing of weather ensembles to reduce bias before entering hydrological applications; • Hydrological model and parameter uncertainty and how to correct and

  19. Probing the cold and warm molecular gas in the Whirlpool Galaxy: Herschel SPIRE-FTS observations of the central region of M51 (NGC 5194)

    Science.gov (United States)

    Schirm, M. R. P.; Wilson, C. D.; Kamenetzky, J.; Parkin, T. J.; Glenn, J.; Maloney, P.; Rangwala, N.; Spinoglio, L.; Baes, M.; Boselli, A.; Cooray, A.; De Looze, I.; Fernández-Ontiveros, J. A.; Karczewski, O. Ł.; Wu, R.

    2017-10-01

    We present Herschel Spectral and Photometric Imaging Receiver (SPIRE)-Fourier Transform Spectrometer (FTS) intermediate-sampled mapping observations of the central ˜8 kpc (˜150 arcsec) of M51, with a spatial resolution of 40 arcsec. We detect four 12CO transitions (J = 4-3 to J = 7-6) and the [C I] 3P2-3P1 and 3P1-3P0 transitions. We supplement these observations with ground-based observations of 12CO J = 1-0 to J = 3-2 and perform a two-component non-local thermodynamic equilibrium analysis. We find that the molecular gas in the nucleus and centre regions has a cool component (Tkin ˜ 10-20 K) with a moderate but poorly constrained density (n(H2) ˜ 103-106 cm-3), as well as significant molecular gas in a warmer (Tkin ˜ 300-3000 K), lower density (n(H2) ˜ 101.6-102.5 cm-3) component. We compare our CO line ratios and calculated densities along with ratios of CO to total infrared luminosity to a grid of photon-dominated region (PDR) models and find that the cold molecular gas likely resides in PDRs with a field strength of G0 ˜ 102. The warm component likely requires an additional source of mechanical heating, from supernovae and stellar winds or possibly shocks produced in the strong spiral density wave. When compared to similar two-component models of other star-forming galaxies published as part of the Very Nearby Galaxies Survey (Arp 220, M82 and NGC 4038/39), M51 has the lowest density for the warm component, while having a warm gas mass fraction that is comparable to those of Arp 220 and M82, and significantly higher than that of NGC 4038/39.

  20. Data management in HEP: An approach

    CERN Document Server

    Furano, F

    2011-01-01

    In this work we describe an approach to data access and data management in High Energy Physics (HEP), which privileges performance, simplicity and scalability, in storage systems that co-operate. We also show why the typical HEP workload is well positioned to access geographically distributed data repositories and then weigh the advantages and disadvantages of accessing data across the Wide Area Network. We discuss some points related to the architecture that a data access/management system should have in order to exploit these possibilities. Currently, this kind of methods were explored by using the xrootd/Scalla software suite, that is a workable example of a distributed non-transactional data repository for the HEP environment. The Scalla architecture naturally allows us to build globally federated repositories congruent with diverse HEP collaborations and their data access needs. These methodologies, however, are based on the concept of caching associated to a performant messaging system and to an efficie...

  1. A modern approach to HEP visualization - ATLASrift

    CERN Document Server

    Vukotic, Ilija; The ATLAS collaboration

    2017-01-01

    At the times when HEP computing needs were mainly fulfilled by mainframes, graphics solutions for event and detector visualizations were necessarily hardware as well as experiment specific and impossible to use anywhere outside of HEP community. A big move to commodity computing did not precipitate a corresponding move of graphics solutions to industry standard hardware and software. In this paper, we list functionalities expected from contemporary tools and describe their implementation by a specific application: ATLASrift. We start with a basic premise that HEP visualization tools should be open in practice and not only in intentions. This means that a user should not be limited to specific and little used platforms, HEP-only software packages, or experiment-specific libraries. Equally important is that no special knowledge or special access rights are needed. Using industry standard frameworks brings not only sustainability, but also good support, a lot of community contributed tools, and a possibility of ...

  2. ASCR/HEP Exascale Requirements Review Report

    Energy Technology Data Exchange (ETDEWEB)

    Habib, Salman [Argonne National Lab. (ANL), Argonne, IL (United States); Roser, Robert [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Gerber, Richard [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Antypas, Katie [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Riley, Katherine [Argonne National Lab. (ANL), Argonne, IL (United States); Williams, Tim [Argonne National Lab. (ANL), Argonne, IL (United States); Wells, Jack [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Straatsma, Tjerk [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Almgren, A. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Amundson, J. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Bailey, S. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Bard, D. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Bloom, K. [Univ. of Nebraska, Lincoln, NE (United States); Bockelman, B. [Univ. of Nebraska, Lincoln, NE (United States); Borgland, A. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Borrill, J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Boughezal, R. [Argonne National Lab. (ANL), Argonne, IL (United States); Brower, R. [Boston Univ., MA (United States); Cowan, B. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Finkel, H. [Argonne National Lab. (ANL), Argonne, IL (United States); Frontiere, N. [Argonne National Lab. (ANL), Argonne, IL (United States); Fuess, S. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Ge, L. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Gnedin, N. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Gottlieb, S. [Indiana Univ., Bloomington, IN (United States); Gutsche, O. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Han, T. [Univ. of Pittsburgh, PA (United States); Heitmann, K. [Argonne National Lab. (ANL), Argonne, IL (United States); Hoeche, S. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Ko, K. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Kononenko, O. [SLAC National Accelerator Lab., Menlo Park, CA (United States); LeCompte, T. [Argonne National Lab. (ANL), Argonne, IL (United States); Li, Z. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Lukic, Z. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Mori, W. [Univ. of California, Los Angeles, CA (United States); Nugent, P. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Ng, C. -K. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Oleynik, G. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); O' Shea, B. [Michigan State Univ., East Lansing, MI (United States); Padmanabhan, N. [Yale Univ., New Haven, CT (United States); Petravick, D. [Univ. of Illinois, Urbana-Champaign, IL (United States). National Center for Supercomputing Applications (NCSA); Petriello, F. J. [Argonne National Lab. (ANL), Argonne, IL (United States); Power, J. [Argonne National Lab. (ANL), Argonne, IL (United States); Qiang, J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Reina, L. [Florida State Univ., Tallahassee, FL (United States); Rizzo, T. J. [SLAC National Accelerator Lab., Menlo Park, CA (United States); Ryne, R. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Schram, M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Spentzouris, P. [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Toussaint, D. [Univ. of Arizona, Tucson, AZ (United States); Vay, J. -L. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Viren, B. [Brookhaven National Lab. (BNL), Upton, NY (United States); Wurthwein, F. [Univ. of California, San Diego, CA (United States); Xiao, L. [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2017-02-06

    This draft report summarizes and details the findings, results, and recommendations derived from the ASCR/HEP Exascale Requirements Review meeting held in June, 2015. The main conclusions are as follows. 1) Larger, more capable computing and data facilities are needed to support HEP science goals in all three frontiers: Energy, Intensity, and Cosmic. The expected scale of the demand at the 2025 timescale is at least two orders of magnitude -- and in some cases greater -- than that available currently. 2) The growth rate of data produced by simulations is overwhelming the current ability, of both facilities and researchers, to store and analyze it. Additional resources and new techniques for data analysis are urgently needed. 3) Data rates and volumes from HEP experimental facilities are also straining the ability to store and analyze large and complex data volumes. Appropriately configured leadership-class facilities can play a transformational role in enabling scientific discovery from these datasets. 4) A close integration of HPC simulation and data analysis will aid greatly in interpreting results from HEP experiments. Such an integration will minimize data movement and facilitate interdependent workflows. 5) Long-range planning between HEP and ASCR will be required to meet HEP's research needs. To best use ASCR HPC resources the experimental HEP program needs a) an established long-term plan for access to ASCR computational and data resources, b) an ability to map workflows onto HPC resources, c) the ability for ASCR facilities to accommodate workflows run by collaborations that can have thousands of individual members, d) to transition codes to the next-generation HPC platforms that will be available at ASCR facilities, e) to build up and train a workforce capable of developing and using simulations and analysis to support HEP scientific research on next-generation systems.

  3. ASCR/HEP Exascale Requirements Review Report

    Energy Technology Data Exchange (ETDEWEB)

    Habib, Salman; et al.

    2016-03-30

    This draft report summarizes and details the findings, results, and recommendations derived from the ASCR/HEP Exascale Requirements Review meeting held in June, 2015. The main conclusions are as follows. 1) Larger, more capable computing and data facilities are needed to support HEP science goals in all three frontiers: Energy, Intensity, and Cosmic. The expected scale of the demand at the 2025 timescale is at least two orders of magnitude -- and in some cases greater -- than that available currently. 2) The growth rate of data produced by simulations is overwhelming the current ability, of both facilities and researchers, to store and analyze it. Additional resources and new techniques for data analysis are urgently needed. 3) Data rates and volumes from HEP experimental facilities are also straining the ability to store and analyze large and complex data volumes. Appropriately configured leadership-class facilities can play a transformational role in enabling scientific discovery from these datasets. 4) A close integration of HPC simulation and data analysis will aid greatly in interpreting results from HEP experiments. Such an integration will minimize data movement and facilitate interdependent workflows. 5) Long-range planning between HEP and ASCR will be required to meet HEP's research needs. To best use ASCR HPC resources the experimental HEP program needs a) an established long-term plan for access to ASCR computational and data resources, b) an ability to map workflows onto HPC resources, c) the ability for ASCR facilities to accommodate workflows run by collaborations that can have thousands of individual members, d) to transition codes to the next-generation HPC platforms that will be available at ASCR facilities, e) to build up and train a workforce capable of developing and using simulations and analysis to support HEP scientific research on next-generation systems.

  4. HEP.TrkX [Vidyo

    CERN Multimedia

    CERN. Geneva

    2017-01-01

    Reconstruction of charged particle tracks is a central task in the processing of physics data at the LHC and other colliders. Current state-of-the-art tracking algorithms are based on the Kalman filter and have seen great success both offline and at trigger level. However, these algorithms scale poorly with increasing detector occupancy, and it is foreseen that significant changes will be needed to achieve efficient track reconstruction in very high luminosity conditions. The HEP.TrkX pilot project aims to develop and explore machine-learning-based algorithms for particle tracking, with the goal of identifying candidate techniques for a more scalable tracking algorithm. In this talk we will discuss the techniques explored in the project so far, with emphasis on algorithms based on recurrent and convolutional neural networks. We will demonstrate the performance of these algorithms on toy detector data, and discuss plans to adapt them into complete algorithms for seed-finding and/or full track reconstructio...

  5. InSpire to Promote Lung Assessment in Youth: Evolving the Self-Management Paradigms of Young People With Asthma.

    Science.gov (United States)

    Elias, Pierre; Rajan, Nithin O; McArthur, Kara; Dacso, Clifford C

    2013-01-01

    Asthma is the most common chronic disease in childhood, disproportionately affecting urban, minority, and disadvantaged children. Individualized care plans supported by daily lung-function monitoring can reduce morbidity and mortality. However, despite 20 years of interventions to increase adherence, only 50% of US youth accurately follow their care plans, which leads to millions of preventable hospitalizations, emergency room visits, and sick days every year. We present a feasibility study of a novel, user-centered approach to increasing young people's lung-function monitoring and asthma self-care. Promoting Lung Assessment in Youth (PLAY) helps young people become active managers of their asthma through the Web 2.0 principles of participation, cocreation, and information sharing. Specifically, PLAY combines an inexpensive, portable spirometer with the motivational power and convenience of mobile phones and virtual-community gaming. The objective of this study was to develop and pilot test InSpire, a fully functional interface between a handheld spirometer and an interactive game and individualized asthma-care instant-messaging system housed on a mobile phone. InSpire is an application for mobile smartphones that creates a compelling world in which youth collaborate with their physicians on managing their asthma. Drawing from design-theory on global timer mechanics and role playing, we incentivized completing spirometry maneuvers by making them an engaging part of a game young people would want to play. The data can be sent wirelessly to health specialists and return care recommendations to patients in real-time. By making it portable and similar to applications normally desired by the target demographic, InSpire is able to seamlessly incorporate asthma management into their lifestyle. We describe the development process of building and testing the InSpire prototype. To our knowledge, the prototype is a first-of-its kind mobile one-stop shop for asthma management

  6. The HEP Software and Computing Knowledge Base

    Science.gov (United States)

    Wenaus, T.

    2017-10-01

    HEP software today is a rich and diverse domain in itself and exists within the mushrooming world of open source software. As HEP software developers and users we can be more productive and effective if our work and our choices are informed by a good knowledge of what others in our community have created or found useful. The HEP Software and Computing Knowledge Base, hepsoftware.org, was created to facilitate this by serving as a collection point and information exchange on software projects and products, services, training, computing facilities, and relating them to the projects, experiments, organizations and science domains that offer them or use them. It was created as a contribution to the HEP Software Foundation, for which a HEP S&C knowledge base was a much requested early deliverable. This contribution will motivate and describe the system, what it offers, its content and contributions both existing and needed, and its implementation (node.js based web service and javascript client app) which has emphasized ease of use for both users and contributors.

  7. DD4Hep based event reconstruction

    CERN Document Server

    AUTHOR|(SzGeCERN)683529; Frank, Markus; Gaede, Frank-Dieter; Hynds, Daniel; Lu, Shaojun; Nikiforou, Nikiforos; Petric, Marko; Simoniello, Rosa; Voutsinas, Georgios Gerasimos

    The DD4HEP detector description toolkit offers a flexible and easy-to-use solution for the consistent and complete description of particle physics detectors in a single system. The sub-component DDREC provides a dedicated interface to the detector geometry as needed for event reconstruction. With DDREC there is no need to define an additional, separate reconstruction geometry as is often done in HEP, but one can transparently extend the existing detailed simulation model to be also used for the reconstruction. Based on the extension mechanism of DD4HEP, DDREC allows one to attach user defined data structures to detector elements at all levels of the geometry hierarchy. These data structures define a high level view onto the detectors describing their physical properties, such as measurement layers, point resolutions, and cell sizes. For the purpose of charged particle track reconstruction, dedicated surface objects can be attached to every volume in the detector geometry. These surfaces provide the measuremen...

  8. Survey of Columbia River Basin Streams for Giant Columbia River Spire Snail Fluminicola columbiana and Great Columbia River limpet Fisherola nuttalli

    Energy Technology Data Exchange (ETDEWEB)

    Neitzel, D.A.; Frest, T.J. (Pacific Northwest Lab., Richland, WA (USA); Washington Univ., Seattle, WA (USA))

    1989-10-01

    Surveys have confirmed the survival of both the giant Columbia River spire snail Fluminicola columbiana and the great Columbia River limpet Fisherola nuttalli in the Hanford Reach of the Columbia River, Washington State, as well as other sites in Washington, Oregon, and Idaho. A review of historical collection records suggests that both species exist in still other sites of the Columbia River Basin. At present, there is insufficient information to allow adequate appraisal of either species relative to possible federal or state listing as endangered or threatened species. The results of our studies suggest that additional undiscovered populations of both species exist. There is a relatively good chance that pristine habitat required by spire snails and limpets remains in 37 streams or portions of streams in Washington, Oregon, Idaho, and Montana (British Columbia was considered outside the project scope). For a thorough survey, visits to more than 600 sites will be required. 20 refs., 5 figs., 7 tabs.

  9. Processing Contexts for Experimental HEP Data

    Energy Technology Data Exchange (ETDEWEB)

    Paterno, Marc [Fermilab; Green, Chris [Fermilab

    2017-02-06

    This document provides, for those not closely associated with the experimental High Energy Physics (HEP) community, an introduction to data input and output requirements for a variety of data processing tasks. Examples in it are drawn from the art event processing framework, and from experiments and projects using art, most notably the LArSoft and NuTools projects.

  10. HEP meets ML award talk : XGBoost

    CERN Multimedia

    CERN. Geneva; CHEN, Tianqi

    2015-01-01

    Tianqi Chen and Tong He (team crowwork) have provided very early in the challenge to all participants XGBoost (for eXtreme Gradient Boosted). It is a parallelised software to train boost decision trees, which has been effectively used by many participants to the challenge. For this, they have won the "HEP meets ML" award which is the invitation to CERN happening today.

  11. Data management in HEP: An approach

    Science.gov (United States)

    Furano, F.

    2011-01-01

    In this work we describe an approach to data access and data management in High Energy Physics (HEP), which privileges performance, simplicity and scalability, in storage systems that co-operate. We also show why the typical HEP workload is well positioned to access geographically distributed data repositories and then weigh the advantages and disadvantages of accessing data across the Wide Area Network. We discuss some points related to the architecture that a data access/management system should have in order to exploit these possibilities. Currently, this kind of methods were explored by using the xrootd/Scalla software suite, that is a workable example of a distributed non-transactional data repository for the HEP environment. The Scalla architecture naturally allows us to build globally federated repositories congruent with diverse HEP collaborations and their data access needs. These methodologies, however, are based on the concept of caching associated to a performant messaging system and to an efficient way of accessing data through Wide Area Network, thus they can be applied to other data access systems as well.

  12. The path toward HEP High Performance Computing

    CERN Document Server

    Apostolakis, John; Carminati, Federico; Gheata, Andrei; Wenzel, Sandro

    2014-01-01

    High Energy Physics code has been known for making poor use of high performance computing architectures. Efforts in optimising HEP code on vector and RISC architectures have yield limited results and recent studies have shown that, on modern architectures, it achieves a performance between 10% and 50% of the peak one. Although several successful attempts have been made to port selected codes on GPUs, no major HEP code suite has a 'High Performance' implementation. With LHC undergoing a major upgrade and a number of challenging experiments on the drawing board, HEP cannot any longer neglect the less-than-optimal performance of its code and it has to try making the best usage of the hardware. This activity is one of the foci of the SFT group at CERN, which hosts, among others, the Root and Geant4 project. The activity of the experiments is shared and coordinated via a Concurrency Forum, where the experience in optimising HEP code is presented and discussed. Another activity is the Geant-V project, centred on th...

  13. Isolation and characterization of microsatellite DNA loci in the threatened flat-spired three-toothed land snail Triodopsis platysayoides

    Science.gov (United States)

    King, Timothy L.; Eackles, Michael S.; Garner, B. A.; van Tuinen, M.; Arbogast, B. S.

    2015-01-01

    The hermaphroditic flat-spired three-tooth land snail (Triodopsis platysayoides) is endemic to a 21-km stretch of the Cheat River Gorge of northeastern West Virginia, USA. We document isolation and characterization of ten microsatellite DNA markers in this at-risk species. The markers displayed a moderate level of allelic diversity (averaging 7.1 alleles/locus) and heterozygosity (averaging 58.6 %). Allelic diversity at seven loci was sufficient to produce unique multilocus genotypes; no indication of selfing was detected in this cosexual species. Minimal deviations from Hardy–Weinberg equilibrium and no linkage disequilibrium were observed within subpopulations. All loci deviated from Hardy–Weinberg expectations when individuals from subpopulations were pooled. Microsatellite markers developed for T. platysayoides yielded sufficient genetic diversity to (1) distinguish all individuals sampled and the level of selfing; (2) be appropriate for addressing fine-scale population structuring; (3) provide novel demographic insights for the species; and (4) cross-amplify and detect allelic diversity in the congeneric T. juxtidens.

  14. Herschel-ATLAS: Dust Temperature and Redshift Distribution of SPIRE and PACS Detected Sources Using Submillimetre Colours

    Science.gov (United States)

    Amblard, A.; Cooray, Asantha; Serra, P.; Temi, P.; Barton, E.; Negrello, M.; Auld, R.; Baes, M.; Baldry, I. K.; Bamford, S.; hide

    2010-01-01

    We present colour-colour diagrams of detected sources in the Herschel-ATLAS Science Demonstration Field from 100 to 500/microns using both PACS and SPIRE. We fit isothermal modified-blackbody spectral energy distribution (SED) models in order to extract the dust temperature of sources with counterparts in GAMA or SDSS with either a spectroscopic or a photometric redshift. For a subsample of 331 sources detected in at least three FIR bands with significance greater than 30 sigma, we find an average dust temperature of (28 plus or minus 8)K. For sources with no known redshifts, we populate the colour-colour diagram with a large number of SEDs generated with a broad range of dust temperatures and emissivity parameters and compare to colours of observed sources to establish the redshift distribution of those samples. For another subsample of 1686 sources with fluxes above 35 mJy at 350 microns and detected at 250 and 500 microns with a significance greater than 3sigma, we find an average redshift of 2.2 plus or minus 0.6.

  15. Inferring Toxicological Responses of HepG2 Cells from ...

    Science.gov (United States)

    Understanding the dynamic perturbation of cell states by chemicals can aid in for predicting their adverse effects. High-content imaging (HCI) was used to measure the state of HepG2 cells over three time points (1, 24, and 72 h) in response to 976 ToxCast chemicals for 10 different concentrations (0.39-200µM). Cell state was characterized by p53 activation (p53), c-Jun activation (SK), phospho-Histone H2A.x (OS), phospho-Histone H3 (MA), alpha tubulin (Mt), mitochondrial membrane potential (MMP), mitochondrial mass (MM), cell cycle arrest (CCA), nuclear size (NS) and cell number (CN). Dynamic cell state perturbations due to each chemical concentration were utilized to infer coarse-grained dependencies between cellular functions as Boolean networks (BNs). BNs were inferred from data in two steps. First, the data for each state variable were discretized into changed/active (> 1 standard deviation), and unchanged/inactive values. Second, the discretized data were used to learn Boolean relationships between variables. In our case, a BN is a wiring diagram between nodes that represent 10 previously described observable phenotypes. Functional relationships between nodes were represented as Boolean functions. We found that inferred BN show that HepG2 cell response is chemical and concentration specific. We observed presence of both point and cycle BN attractors. In addition, there are instances where Boolean functions were not found. We believe that this may be either

  16. HEP Science Network Requirements--Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Bakken, Jon; Barczyk, Artur; Blatecky, Alan; Boehnlein, Amber; Carlson, Rich; Chekanov, Sergei; Cotter, Steve; Cottrell, Les; Crawford, Glen; Crawford, Matt; Dart, Eli; Dattoria, Vince; Ernst, Michael; Fisk, Ian; Gardner, Rob; Johnston, Bill; Kent, Steve; Lammel, Stephan; Loken, Stewart; Metzger, Joe; Mount, Richard; Ndousse-Fetter, Thomas; Newman, Harvey; Schopf, Jennifer; Sekine, Yukiko; Stone, Alan; Tierney, Brian; Tull, Craig; Zurawski, Jason

    2010-04-27

    The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the US Department of Energy Office of Science, the single largest supporter of basic research in the physical sciences in the United States. In support of the Office of Science programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet to be a highly successful enabler of scientific discovery for over 20 years. In August 2009 ESnet and the Office of High Energy Physics (HEP), of the DOE Office of Science, organized a workshop to characterize the networking requirements of the programs funded by HEP. The International HEP community has been a leader in data intensive science from the beginning. HEP data sets have historically been the largest of all scientific data sets, and the communty of interest the most distributed. The HEP community was also the first to embrace Grid technologies. The requirements identified at the workshop are summarized below, and described in more detail in the case studies and the Findings section: (1) There will be more LHC Tier-3 sites than orginally thought, and likely more Tier-2 to Tier-2 traffic than was envisioned. It it not yet known what the impact of this will be on ESnet, but we will need to keep an eye on this traffic. (2) The LHC Tier-1 sites (BNL and FNAL) predict the need for 40-50 Gbps of data movement capacity in 2-5 years, and 100-200 Gbps in 5-10 years for HEP program related traffic. Other key HEP sites include LHC Tier-2 and Tier-3 sites, many of which are located at universities. To support the LHC, ESnet must continue its collaborations with university and international networks. (3) While in all cases the deployed 'raw' network bandwidth must exceed the user requirements in order to meet the data transfer and reliability requirements, network engineering for trans

  17. A magnet system for HEP experiments

    CERN Document Server

    Gaddi, A

    2012-01-01

    This chapter describes the sequence of steps that lead to the design of a magnet system for modern HEP detectors. We start looking to the main types of magnets used in HEP experiments, along with some basic formulae to set the main parameters, such as ampere-turns, impedance and stored energy. A section is dedicated to the description of the iron yoke, with emphasis on magnet-detector integration and assembly, steel characteristics, stray field issues and alternative design. In the second part of the chapter we start looking at a brief history of superconducting magnets and a comparison between warm and superconducting ones. Following that, we describe the commonly used superconducting cables, the conductor design and technology and the winding techniques. A section of the chapter is dedicated to the cryogenic design, vacuum insulation and other ancillary systems. We also describe the power circuit, with the power supply unit, the current leads, the current measurement devices and other instruments and safety...

  18. Support vector machines and generalisation in HEP

    Science.gov (United States)

    Bevan, Adrian; Gamboa Goñi, Rodrigo; Hays, Jon; Stevenson, Tom

    2017-10-01

    We review the concept of Support Vector Machines (SVMs) and discuss examples of their use in a number of scenarios. Several SVM implementations have been used in HEP and we exemplify this algorithm using the Toolkit for Multivariate Analysis (TMVA) implementation. We discuss examples relevant to HEP including background suppression for H → τ + τ ‑ at the LHC with several different kernel functions. Performance benchmarking leads to the issue of generalisation of hyper-parameter selection. The avoidance of fine tuning (over training or over fitting) in MVA hyper-parameter optimisation, i.e. the ability to ensure generalised performance of an MVA that is independent of the training, validation and test samples, is of utmost importance. We discuss this issue and compare and contrast performance of hold-out and k-fold cross-validation. We have extended the SVM functionality and introduced tools to facilitate cross validation in TMVA and present results based on these improvements.

  19. HEP Science Network Requirements. Final Report

    International Nuclear Information System (INIS)

    Dart, Eli; Tierney, Brian

    2010-01-01

    The Energy Sciences Network (ESnet) is the primary provider of network connectivity for the US Department of Energy Office of Science, the single largest supporter of basic research in the physical sciences in the United States. In support of the Office of Science programs, ESnet regularly updates and refreshes its understanding of the networking requirements of the instruments, facilities, scientists, and science programs that it serves. This focus has helped ESnet to be a highly successful enabler of scientific discovery for over 20 years. In August 2009 ESnet and the Office of High Energy Physics (HEP), of the DOE Office of Science, organized a workshop to characterize the networking requirements of the programs funded by HEP. The International HEP community has been a leader in data intensive science from the beginning. HEP data sets have historically been the largest of all scientific data sets, and the communty of interest the most distributed. The HEP community was also the first to embrace Grid technologies. The requirements identified at the workshop are summarized below, and described in more detail in the case studies and the Findings section: (1) There will be more LHC Tier-3 sites than orginally thought, and likely more Tier-2 to Tier-2 traffic than was envisioned. It it not yet known what the impact of this will be on ESnet, but we will need to keep an eye on this traffic. (2) The LHC Tier-1 sites (BNL and FNAL) predict the need for 40-50 Gbps of data movement capacity in 2-5 years, and 100-200 Gbps in 5-10 years for HEP program related traffic. Other key HEP sites include LHC Tier-2 and Tier-3 sites, many of which are located at universities. To support the LHC, ESnet must continue its collaborations with university and international networks. (3) While in all cases the deployed 'raw' network bandwidth must exceed the user requirements in order to meet the data transfer and reliability requirements, network engineering for trans-Atlantic connectivity

  20. The path toward HEP High Performance Computing

    International Nuclear Information System (INIS)

    Apostolakis, John; Brun, René; Gheata, Andrei; Wenzel, Sandro; Carminati, Federico

    2014-01-01

    High Energy Physics code has been known for making poor use of high performance computing architectures. Efforts in optimising HEP code on vector and RISC architectures have yield limited results and recent studies have shown that, on modern architectures, it achieves a performance between 10% and 50% of the peak one. Although several successful attempts have been made to port selected codes on GPUs, no major HEP code suite has a 'High Performance' implementation. With LHC undergoing a major upgrade and a number of challenging experiments on the drawing board, HEP cannot any longer neglect the less-than-optimal performance of its code and it has to try making the best usage of the hardware. This activity is one of the foci of the SFT group at CERN, which hosts, among others, the Root and Geant4 project. The activity of the experiments is shared and coordinated via a Concurrency Forum, where the experience in optimising HEP code is presented and discussed. Another activity is the Geant-V project, centred on the development of a highperformance prototype for particle transport. Achieving a good concurrency level on the emerging parallel architectures without a complete redesign of the framework can only be done by parallelizing at event level, or with a much larger effort at track level. Apart the shareable data structures, this typically implies a multiplication factor in terms of memory consumption compared to the single threaded version, together with sub-optimal handling of event processing tails. Besides this, the low level instruction pipelining of modern processors cannot be used efficiently to speedup the program. We have implemented a framework that allows scheduling vectors of particles to an arbitrary number of computing resources in a fine grain parallel approach. The talk will review the current optimisation activities within the SFT group with a particular emphasis on the development perspectives towards a simulation framework able to profit

  1. Future HEP Accelerators: The US Perspective

    Energy Technology Data Exchange (ETDEWEB)

    Bhat, Pushpalatha [Fermilab; Shiltsev, Vladimir [Fermilab

    2015-11-02

    Accelerator technology has advanced tremendously since the introduction of accelerators in the 1930s, and particle accelerators have become indispensable instruments in high energy physics (HEP) research to probe Nature at smaller and smaller distances. At present, accelerator facilities can be classified into Energy Frontier colliders that enable direct discoveries and studies of high mass scale particles and Intensity Frontier accelerators for exploration of extremely rare processes, usually at relatively low energies. The near term strategies of the global energy frontier particle physics community are centered on fully exploiting the physics potential of the Large Hadron Collider (LHC) at CERN through its high-luminosity upgrade (HL-LHC), while the intensity frontier HEP research is focused on studies of neutrinos at the MW-scale beam power accelerator facilities, such as Fermilab Main Injector with the planned PIP-II SRF linac project. A number of next generation accelerator facilities have been proposed and are currently under consideration for the medium- and long-term future programs of accelerator-based HEP research. In this paper, we briefly review the post-LHC energy frontier options, both for lepton and hadron colliders in various regions of the world, as well as possible future intensity frontier accelerator facilities.

  2. Hemangioendotelioma hepático del lactante

    Directory of Open Access Journals (Sweden)

    Juan Carlos Jara Santamaría

    2018-03-01

    Full Text Available Introducción: El hemangioendotelioma hepático infantil es una afectación poco común que se caracteriza por el desarrollo de una o varias neoplasias de origen vascular en el hígado. Dichas neoplasias generalmente son benignas, aunque tienen un potencial intermedio de transformarse en tumores malignos.  Objetivo: Dar a conocer el caso de una patología de presentación inusual, enfatizar su importancia dentro de los diagnósticos diferenciales en estudios radiológicos con tomografía computarizada y ecografía abdominal. Descripción del caso: En el siguiente caso se presenta a un paciente lactante de dos meses de edad, nacido a las 38 semanas de gestación sin ninguna complicación aparente. Tras presentarse a la consulta debido al desarrollo de síntomas propios de la enfermedad, y tras realizar estudios de imagen, tomografía computarizada multicorte corporal y ecografía abdominal, se determinó que el paciente padece de múltiples hemangioendoteliomas hepáticos, sometido a terapia farmacológica y respiratoria además tratamiento intervencionista como embolización, sin tener mejoría, complicándose su cuadro clínico con insuficiencia cardiaca rara en esta patología y progresa hasta su fallecimiento. Conclusiones: El diagnóstico de Hemangioendotelioma Hepático del lactante se puede hacer sin la exigencia de una confirmación histológica cuando los hallazgos clínicos, analíticos y de imagen son muy sugestivos. Es raro el curso de esta enfermedad con Insuficiencia cardiaca y fallecimiento del lactante.

  3. Detector Simulations with DD4hep

    Science.gov (United States)

    Petrič, M.; Frank, M.; Gaede, F.; Lu, S.; Nikiforou, N.; Sailer, A.

    2017-10-01

    Detector description is a key component of detector design studies, test beam analyses, and most of particle physics experiments that require the simulation of more and more different detector geometries and event types. This paper describes DD4hep, which is an easy-to-use yet flexible and powerful detector description framework that can be used for detector simulation and also extended to specific needs for a particular working environment. Linear collider detector concepts ILD, SiD and CLICdp as well as detector development collaborations CALICE and FCal have chosen to adopt the DD4hep geometry framework and its DDG4 pathway to Geant4 as its core simulation and reconstruction tools. The DDG4 plugins suite includes a wide variety of input formats, provides access to the Geant4 particle gun or general particles source and allows for handling of Monte Carlo truth information, eg. by linking hits and the primary particle that caused them, which is indispensable for performance and efficiency studies. An extendable array of segmentations and sensitive detectors allows the simulation of a wide variety of detector technologies. This paper shows how DD4hep allows to perform complex Geant4 detector simulations without compiling a single line of additional code by providing a palette of sub-detector components that can be combined and configured via compact XML files. Simulation is controlled either completely via the command line or via simple Python steering files interpreted by a Python executable. It also discusses how additional plugins and extensions can be created to increase the functionality.

  4. Sarcoma de Kaposi relacionado à síndrome da imunodeficiência adquirida: características do comprometimento hepático na tomografia computadorizada e na ressonância magnética Kaposi sarcoma related to acquired immunodeficiency syndrome: hepatic findings on computed tomography and magnetic resonance imaging

    Directory of Open Access Journals (Sweden)

    Daniel Nobrega da Costa

    2008-04-01

    Full Text Available Sarcoma de Kaposi é uma neoplasia associada a condições de imunossupressão que acomete os vasos linfáticos e sanguíneos. É a neoplasia intra-hepática mais comum na síndrome da imunodeficiência adquirida. A tomografia computadorizada e a ressonância magnética revelam múltiplos pequenos nódulos, proeminência e realce dos planos periportais, devido à presença de tecido neoplásico. Os autores descrevem um caso de paciente masculino, de 47 anos de idade, com síndrome da imunodeficiência adquirida e sarcoma de Kaposi disseminado.Kaposi sarcoma is a neoplasm associated with immunosuppressive conditions, and involving blood and lymphatic vessels. It is the most frequent intrahepatic neoplasm in patients with acquired immunodeficiency syndrome. Computed tomography and magnetic resonance imaging demonstrate multiple small nodules, prominence and contrast-enhancement of periportal branches due to the presence of the neoplastic tissue. The authors report a case of a 47-year-old male patient with acquired immunodeficiency syndrome presenting disseminated Kaposi sarcoma.

  5. Totem a case study in HEP

    CERN Document Server

    Dusini, S; Lazzizzera, I; Lee, P; Sartori, A; Sidoti, A; Tecchiolli, G P; Zorat, A

    1997-01-01

    It is being proved that the neurochip \\Totem{} is a viable solution for high quality and real time computational tasks in HEP, including event classification, triggering and signal processing. The architecture of the chip is based on a "derivative free" algorithm called Reactive Tabu Search (RTS), highly performing even for low precision weights. ISA, VME or PCI boards integrate the chip as a coprocessor in a host computer. This paper presents: 1) the state of the art and the next evolution of the design of \\Totem{}; 2) its ability in the Higgs search at LHC as an example.

  6. From Physics to industry: EOS outside HEP

    Science.gov (United States)

    Espinal, X.; Lamanna, M.

    2017-10-01

    In the competitive market for large-scale storage solutions the current main disk storage system at CERN EOS has been showing its excellence in the multi-Petabyte high-concurrency regime. It has also shown a disruptive potential in powering the service in providing sync and share capabilities and in supporting innovative analysis environments along the storage of LHC data. EOS has also generated interest as generic storage solution ranging from university systems to very large installations for non-HEP applications.

  7. Estudo comparativo dos procedimentos percutâneos orientados por métodos de imagem no tratamento das coleções hepáticas A comparative study of image-guided percutaneous procedures for the treatment of liver abscesses

    Directory of Open Access Journals (Sweden)

    Giselle Guedes Netto de Mello

    2001-04-01

    Full Text Available OBJETIVO: Verificar e comparar a eficácia da punção aspirativa e da drenagem percutânea, orientadas por métodos de imagem, no tratamento das coleções hepáticas. MATERIAIS E MÉTODOS: Foram encaminhados, ao nosso setor, 52 pacientes para tratamento percutâneo de coleções abdominais. Destes, 17 apresentavam coleções hepáticas, sendo 13/17 não-complexas e 4/17, complexas (multiloculadas, múltiplas ou associadas a fístulas. Foram submetidas a punção aspirativa 7/17 coleções (41,2% e a drenagem percutânea, 10/17 (58,8%. Considerou-se como sucesso do método a resolução completa da coleção, com melhora clínica e laboratorial do paciente. RESULTADOS: O sucesso do procedimento ocorreu em 82,4% dos casos. Nas coleções submetidas exclusivamente a punção aspirativa, obteve-se sucesso em 57,1%, enquanto nas submetidas a drenagem percutânea o sucesso foi de 100%. O índice de sucesso no grupo submetido a punção aspirativa foi de 75% nas coleções menores que 100 ml, e de 33,3% naquelas entre 100 e 250 ml. Houve sucesso com a punção aspirativa em 75% das coleções não-complexas e em 25% das complexas. CONCLUSÃO: A drenagem percutânea é mais eficaz que a punção aspirativa no tratamento das coleções hepáticas. A punção aspirativa talvez possa ser empregada como alternativa válida nas coleções de menor volume e não-complexas.OBJECTIVE: To determine and compare the efficacy of percutaneous needle aspiration and percutaneous catheter drainage, both guided by imaging methods, for the treatment of liver abscesses. MATERIALS AND METHODS: From 52 patients referred to our service for percutaneous treatment of abdominal abscesses, 17 presented liver abscesses, 13 of which were considered noncomplex and four were considered complex (multiloculated, multiple or associated to fistulas. Percutaneous needle aspiration was performed in 7/17 patients and 10/17 patients were submitted to percutaneous catheter drainage. The

  8. Dynamic provisioning of a HEP computing infrastructure on a shared hybrid HPC system

    International Nuclear Information System (INIS)

    Meier, Konrad; Fleig, Georg; Hauth, Thomas; Quast, Günter; Janczyk, Michael; Von Suchodoletz, Dirk; Wiebelt, Bernd

    2016-01-01

    Experiments in high-energy physics (HEP) rely on elaborate hardware, software and computing systems to sustain the high data rates necessary to study rare physics processes. The Institut fr Experimentelle Kernphysik (EKP) at KIT is a member of the CMS and Belle II experiments, located at the LHC and the Super-KEKB accelerators, respectively. These detectors share the requirement, that enormous amounts of measurement data must be processed and analyzed and a comparable amount of simulated events is required to compare experimental results with theoretical predictions. Classical HEP computing centers are dedicated sites which support multiple experiments and have the required software pre-installed. Nowadays, funding agencies encourage research groups to participate in shared HPC cluster models, where scientist from different domains use the same hardware to increase synergies. This shared usage proves to be challenging for HEP groups, due to their specialized software setup which includes a custom OS (often Scientific Linux), libraries and applications. To overcome this hurdle, the EKP and data center team of the University of Freiburg have developed a system to enable the HEP use case on a shared HPC cluster. To achieve this, an OpenStack-based virtualization layer is installed on top of a bare-metal cluster. While other user groups can run their batch jobs via the Moab workload manager directly on bare-metal, HEP users can request virtual machines with a specialized machine image which contains a dedicated operating system and software stack. In contrast to similar installations, in this hybrid setup, no static partitioning of the cluster into a physical and virtualized segment is required. As a unique feature, the placement of the virtual machine on the cluster nodes is scheduled by Moab and the job lifetime is coupled to the lifetime of the virtual machine. This allows for a seamless integration with the jobs sent by other user groups and honors the fairshare

  9. Detector simulations with DD4hep

    CERN Document Server

    AUTHOR|(SzGeCERN)668365; Frank, Markus; Gaede, Frank-Dieter; Lu, Shaojun; Nikiforou, Nikiforos; Sailer, Andre

    2017-01-01

    Detector description is a key component of detector design studies, test beam analyses, and most of particle physics experiments that require the simulation of more and more different detector geometries and event types. This paper describes DD4hep, which is an easy-to-use yet flexible and powerful detector description framework that can be used for detector simulation and also extended to specific needs for a particular working environment. Linear collider detector concepts ILD, SiD and CLICdp as well as detector development collaborations CALICE and FCal have chosen to adopt the DD4hep geometry framework and its DDG4 pathway to Geant4 as its core simulation and reconstruction tools. The DDG4 plugins suite includes a wide variety of input formats, provides access to the Geant4 particle gun or general particles source and allows for handling of Monte Carlo truth information, e.g. by linking hits and the primary particle that caused them, which is indispensable for performance and efficiency studies. An extend...

  10. II - Multivariate Classification and Machine Learning in HEP

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    A summary of the history of deep-learning is given and the difference to traditional artificial neural networks is discussed. Advanced methods like convoluted neural networks, recurrent neural networks and unsupervised training are introduced. Interesting examples from this emerging field outside HEP are presented. Possible applications in HEP are discussed.

  11. Synthesis and Evaluation of New Pyrazoline Derivatives as Potential Anticancer Agents in HepG-2 Cell Line

    Directory of Open Access Journals (Sweden)

    Weijie Xu

    2017-03-01

    Full Text Available Cancer is a major public health concern worldwide. Adverse effects of cancer treatments still compromise patients’ quality of life. To identify new potential anticancer agents, a series of novel pyrazoline derivatives were synthesized and evaluated for cytotoxic effects on HepG-2 (human liver hepatocellular carcinoma cell line and primary hepatocytes. Compound structures were confirmed by 1H-NMR, mass spectrometry, and infrared imaging. An in vitro assay demonstrated that several compounds exerted cytotoxicity in the micromolar range. Benzo[b]thiophen-2-yl-[5-(4-hydroxy-3,5-dimethoxy-phenyl-3-(2-hydroxy-phenyl-4,5-dihydo-pyrazol-1-yl]-methanone (b17 was the most effective anticancer agent against HepG-2 cells owing to its notable inhibitory effect on HepG-2 with an IC50 value of 3.57 µM when compared with cisplatin (IC50 = 8.45 µM and low cytotoxicity against primary hepatocytes. Cell cycle analysis and apoptosis/necrosis evaluation using this compound revealed that b17 notably arrested HepG-2 cells in the G2/M phase and induced HepG-2 cells apoptosis. Our findings indicate that compound b17 may be a promising anticancer drug candidate.

  12. Oxford exploding spires.

    Science.gov (United States)

    Guest, I

    1974-05-01

    Barbara Ward in her keynote address to the Conference on Exploding Cities, held in Oxford England, made the point that the current problems of the cities can only worsen as the world's population doubles by the year 2000 and the worst hit will be the cities of the developing nations. Between 1950 and 2000 urban populations will rise from 500 million to 1000 million, but the equivalent rise in the 3rd world will be from 500 million to 2200 million. The Conference was more successful in identifying the problems and their causes than in proposing solutions. The inadequacy of urban transport was emphasized repeatedly. Crime emerges as another urban headache. Pollution was another popular theme, and shanties were thoroughly examined. Positive proposals too often took the form of self-evident truths. In all cases the message is clear: the 1st prerequisite to urban planning is central control and, if necessary, public ownership of land. A succession of speakers advocated more self-help for the communities which have emerged on the periphery of so many urban centers. Elizabeth Jelin, from Buenos Aires, argued that these communities -- and shanty towns are the most obvious manifestation -- should no longer be viewed as marginal. They perform essential services, especially in terms of employment, and cannot be moved without severe disruption. Self-help was the beacon held up to delegates, despite the warnings that it would lead to complacency on the part of authorities.

  13. Alcoolismo e cirrose hepática

    OpenAIRE

    Gonçalves, Lisa Isabel Baptista

    2009-01-01

    Introdução: A despeito do desenvolvimento científico e tecnológico, ocorrido nas últimas décadas e o facto de todo o conhecimento fisiopatológico da cirrose hepática ter uma marcada influência global e empírica, o avanço na terapêutica tem sido lento, mantendo-se inúmeras áreas “cinzentas”. Concomitantemente, às elevadas taxas de mortalidade numa população demasiado jovem e activa e às taxas de morbilidade que levam a custos económico-sociais crescentes, faz com seja fundamental não só empenh...

  14. Data reduction systems for HEP experiments

    CERN Document Server

    Jaroslawski, S

    1980-01-01

    Fast logic triggers are described which are capable of rejecting unwanted events during HEP experiments. Classes of track patterns are defined by entries in read/write memories and can be easily changed. Readout information from multiwire chambers addresses these memories and their outputs are processed by fast combinatorial logic to produce trigger decisions. In the circumstances where speed of decision making is not the prime consideration a much cheaper serial-parallel decision sequence may be implemented which, although slower in operation, can offer a very good definition of the tracks generated in a given data intake. Triggers of both categories are currently being used in experiments mounted at DESY and at CERN. (3 refs).

  15. Cytotoxicity and apoptotic effects of tea polyphenol-loaded chitosan nanoparticles on human hepatoma HepG2 cells

    International Nuclear Information System (INIS)

    Liang, Jin; Li, Feng; Fang, Yong; Yang, Wenjian; An, Xinxin; Zhao, Liyan; Xin, Zhihong; Cao, Lin; Hu, Qiuhui

    2014-01-01

    Tea polyphenols have strong antioxidant and antitumor activities. However, these health benefits are limited due to their poor in vivo stability and low bioavailability. Chitosan nanoparticles as delivery systems may provide an alternative approach for enhancing bioavailability of poorly absorbed drugs. In this study, tea polyphenol-loaded chitosan nanoparticles have been prepared using two different chitosan biomaterials, and their antitumor effects were evaluated in HepG2 cells, including cell cytotoxicity comparison, cell morphology analysis, cell apoptosis and cell cycle detection. The results indicated that the tea polyphenol-loaded chitosan nanoparticles showed a branch shape and heterogeneous distribution in prepared suspension. MTT assay suggested that tea polyphenol-loaded chitosan nanoparticles could inhibit the proliferation of HepG2 cells, and the cytotoxicity rates were increased gradually and appeared an obvious dose-dependent relationship. Transmission electron microscope images showed that the HepG2 cells treated with tea polyphenol-loaded chitosan nanoparticles exhibited some typical apoptotic features, such as microvilli disappearance, margination of nuclear chromatin, intracytoplasmic vacuoles and the mitochondrial swelling. In addition, the tea polyphenol-loaded chitosan nanoparticles had relatively weak inhibitory effects on HepG2 cancer cells compared with tea polyphenols. Tea polyphenols not only induced cancer cell apoptosis, but also promoted their necrosis. However, tea polyphenol-loaded chitosan nanoparticles exhibited their antitumor effects mainly through inducing cell apoptosis. Our results revealed that the inhibition effects of tea polyphenol-loaded chitosan nanoparticles on tumor cells probably depended on their controlled drug release and effective cell delivery. The chitosan nanoparticles themselves as the delivery carrier showed limited antitumor effects compared with their encapsulated drugs. - Highlights: • Tea polyphenol

  16. Cytotoxicity and apoptotic effects of tea polyphenol-loaded chitosan nanoparticles on human hepatoma HepG2 cells

    Energy Technology Data Exchange (ETDEWEB)

    Liang, Jin [Key Laboratory of Tea Biochemistry and Biotechnology of Ministry of Education and Ministry of Agriculture, Anhui Agricultural University, Hefei 230036 (China); College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Li, Feng [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Fang, Yong; Yang, Wenjian [College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023 (China); An, Xinxin; Zhao, Liyan; Xin, Zhihong; Cao, Lin [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); Hu, Qiuhui, E-mail: qiuhuihu@njau.edu.cn [College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095 (China); College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023 (China)

    2014-03-01

    Tea polyphenols have strong antioxidant and antitumor activities. However, these health benefits are limited due to their poor in vivo stability and low bioavailability. Chitosan nanoparticles as delivery systems may provide an alternative approach for enhancing bioavailability of poorly absorbed drugs. In this study, tea polyphenol-loaded chitosan nanoparticles have been prepared using two different chitosan biomaterials, and their antitumor effects were evaluated in HepG2 cells, including cell cytotoxicity comparison, cell morphology analysis, cell apoptosis and cell cycle detection. The results indicated that the tea polyphenol-loaded chitosan nanoparticles showed a branch shape and heterogeneous distribution in prepared suspension. MTT assay suggested that tea polyphenol-loaded chitosan nanoparticles could inhibit the proliferation of HepG2 cells, and the cytotoxicity rates were increased gradually and appeared an obvious dose-dependent relationship. Transmission electron microscope images showed that the HepG2 cells treated with tea polyphenol-loaded chitosan nanoparticles exhibited some typical apoptotic features, such as microvilli disappearance, margination of nuclear chromatin, intracytoplasmic vacuoles and the mitochondrial swelling. In addition, the tea polyphenol-loaded chitosan nanoparticles had relatively weak inhibitory effects on HepG2 cancer cells compared with tea polyphenols. Tea polyphenols not only induced cancer cell apoptosis, but also promoted their necrosis. However, tea polyphenol-loaded chitosan nanoparticles exhibited their antitumor effects mainly through inducing cell apoptosis. Our results revealed that the inhibition effects of tea polyphenol-loaded chitosan nanoparticles on tumor cells probably depended on their controlled drug release and effective cell delivery. The chitosan nanoparticles themselves as the delivery carrier showed limited antitumor effects compared with their encapsulated drugs. - Highlights: • Tea polyphenol

  17. Study of a Docker Use-Case for HEP

    Energy Technology Data Exchange (ETDEWEB)

    Kowalkowski, Jim [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Lyon, Adam [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Paterno, Marc [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2016-01-01

    Over the past few years, container technology has become increasingly promising as a means to seamlessly make our software available across a wider range of platforms. In December 2015, we decided to put together a set of docker images that serve as a demonstration of this container technology for managing a run-time environment for art-related software projects, and also serve as a set of test cases for evaluation of performance. Docker[1] containers provide a way to “wrap up a piece of software in a complete filesystem that contains everything it needs to run”. In combination with Shifter[2], such containers provide a way to run software developed and deployed on “typical” HEP platforms (such as SLF 6, in common use at Fermilab and on OSG platforms) on HPC facilities at NERSC. Docker containers provide a means of delivering software that can be run on a variety of hosts without needing to be compiled specially for each OS to be supported. This could substantially reduce the effort required to create and validate a new release, since one build could be suitable for use on both grid machines (both FermiGrid and OSG) as well as any machine capable of running the Docker container. In addition, docker containers may provide for a quick and easy way for users to install and use a software release in a standardized environment. This report contains the results and status of this demonstration and evaluation.

  18. UARS PEM Level 2 HEPS A V001

    Data.gov (United States)

    National Aeronautics and Space Administration — The Particle Environment Monitor (PEM) level 2 High-Energy Particle Spectrometer (HEPS) A daily product contains electron high-resolution spectral data converted to...

  19. UARS PEM Level 2 HEPS B V001

    Data.gov (United States)

    National Aeronautics and Space Administration — The Particle Environment Monitor (PEM) level 2 High-Energy Particle Spectrometer (HEPS) B daily product contains the electron high-resolution spectral data converted...

  20. Deploying HEP applications using Xen and Globus Virtual Workspaces

    International Nuclear Information System (INIS)

    Agarwal, A; Desmarais, R; Gable, I; Grundy, D; P-Brown, D; Seuster, R; Vanderster, D C; Sobie, R; Charbonneau, A; Enge, R

    2008-01-01

    The deployment of HEP applications in heterogeneous grid environments can be challenging because many of the applications are dependent on specific OS versions and have a large number of complex software dependencies. Virtual machine monitors such as Xen could be used to package HEP applications, complete with their execution environments, to run on resources that do not meet their operating system requirements. Our previous work has shown HEP applications running within Xen suffer little or no performance penalty as a result of virtualization. However, a practical strategy is required for remotely deploying, booting, and controlling virtual machines on a remote cluster. One tool that promises to overcome the deployment hurdles using standard grid technology is the Globus Virtual Workspaces project. We describe strategies for the deployment of Xen virtual machines using Globus Virtual Workspace middleware that simplify the deployment of HEP applications

  1. Complicações hepáticas na doença falciforme Hepatic complications in sickle cell disease

    Directory of Open Access Journals (Sweden)

    Fabíola Traina

    2007-09-01

    Full Text Available Doentes falciformes podem apresentar alterações hepáticas agudas ou crônicas. As agudas são caracterizadas por dor no quadrante superior direito e icterícia. O diagnóstico diferencial inclui crise aguda de falcização hepática, seqüestro hepático, colestase intra-hepática, colelitíase, coledocolitíase, colecistite e hepatite viral aguda. Estas complicações devem ser diagnosticadas precocemente, através de história clínica, testes de função hepática e exames radiológicos, e o tratamento deve ser prontamente iniciado. Transfusão sangüínea é essencial para o tratamento das manifestações agudas causadas pelo processo de vaso-oclusão, como seqüestro hepático e colestase intra-hepática. As alterações hepáticas crônicas são freqüentemente causadas pela hemólise crônica e múltiplas transfusões. Para prevenção, diagnóstico precoce e orientação terapêutica da alteração hepática crônica, os doentes falciformes devem ser submetidos a exames de rotina: testes de função hepática, sorologia para hepatite B e C, dosagem sérica de ferritina e ultra-sonografia de abdômen. A biópsia hepática deve ser realizada em pacientes com hepatite viral e em pacientes com alterações hepáticas crônicas acentuadas e persistentes, afora das manifestações agudas.Patients with sickle cell disease may present acute or chronic hepatopathy. The acute syndrome is characterized by right upper quadrant abdominal pain and jaundice. The differential diagnoses include acute sickle hepatic crises, hepatic sequestration, sickle cell intrahepatic cholestasis, cholecystitis, choledocholithiasis and acute viral hepatitis. These alterations can be differentiated by a careful history, liver function tests and hepatobiliary imaging studies. The specific treatment must be promptly initiated. Red blood cell transfusion is essential for the treatment of the clinical syndromes caused by the sickling process such as hepatic sequestration

  2. NiMax system for hadronic event generators in HEP

    International Nuclear Information System (INIS)

    Amelin, N.S.; Komogorov, M.E.

    2001-01-01

    We have suggested a new approach to the development and use of Monte Carlo event generators in high-energy physics (HEP). It is a component approach, when a complex numerical model is composed of standard components. Our approach opens a way to organize a library of HEP model components and provides a great flexibility for the construction of very powerful and realistic numerical models. To support this approach we have designed the NiMax software system (framework) written in C++

  3. Identifying Memory Allocation Patterns in HEP Software

    Science.gov (United States)

    Kama, S.; Rauschmayr, N.

    2017-10-01

    HEP applications perform an excessive amount of allocations/deallocations within short time intervals which results in memory churn, poor locality and performance degradation. These issues are already known for a decade, but due to the complexity of software frameworks and billions of allocations for a single job, up until recently no efficient mechanism has been available to correlate these issues with source code lines. However, with the advent of the Big Data era, many tools and platforms are now available to do large scale memory profiling. This paper presents, a prototype program developed to track and identify each single (de-)allocation. The CERN IT Hadoop cluster is used to compute memory key metrics, like locality, variation, lifetime and density of allocations. The prototype further provides a web based visualization back-end that allows the user to explore the results generated on the Hadoop cluster. Plotting these metrics for every single allocation over time gives a new insight into application’s memory handling. For instance, it shows which algorithms cause which kind of memory allocation patterns, which function flow causes how many short-lived objects, what are the most commonly allocated sizes etc. The paper will give an insight into the prototype and will show profiling examples for the LHC reconstruction, digitization and simulation jobs.

  4. Experience with HEP analysis on mounted filesystems

    International Nuclear Information System (INIS)

    Fuhrmann, Patrick; Gasthuber, Martin; Kemp, Yves; Ozerov, Dmitry

    2012-01-01

    We present results on different approaches on mounted filesystems in use or under investigation at DESY. dCache, established since long as a storage system for physics data has implemented the NFS v4.1/pNFS protocol. New performance results will be shown with the most current version of the dCache server. In addition to the native usage of the mounted filesystem in a LAN environment, the results are given for the performance of the dCache NFS v4.1/pNFS in WAN case. Several commercial vendors are currently in alpha or beta phase of adding the NFS v4.1/pNFS protocol to their storage appliances. We will test some of these vendor solutions for their readiness for HEP analysis. DESY has recently purchased an IBM Sonas system. We will present the result of a thorough performance evaluation using the native protocols NFS (v3 or v4) and GPFS. As the emphasis is on the usability for end user analysis, we will use latest ROOT versions and current end user analysis code for benchmark scenarios.

  5. West Foster Creek Expansion Project 2007 HEP Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-02-01

    During April and May 2007, the Columbia Basin Fish and Wildlife Authority's (CBFWA) Regional HEP Team (RHT) conducted baseline Habitat Evaluation Procedures (HEP) (USFWS 1980, 1980a) analyses on five parcels collectively designated the West Foster Creek Expansion Project (3,756.48 acres). The purpose of the HEP analyses was to document extant habitat conditions and to determine how many baseline/protection habitat units (HUs) to credit Bonneville Power Administration (BPA) for funding maintenance and enhancement activities on project lands as partial mitigation for habitat losses associated with construction of Grand Coulee and Chief Joseph Dams. HEP evaluation models included mule deer (Odocoileus hemionus), western meadowlark (Sturnella neglecta), sharp-tailed grouse, (Tympanuchus phasianellus), Bobcat (Lynx rufus), mink (Neovison vison), mallard (Anas platyrhynchos), and black-capped chickadee (Parus atricapillus). Combined 2007 baseline HEP results show that 4,946.44 habitat units were generated on 3,756.48 acres (1.32 HUs per acre). HEP results/habitat conditions were generally similar for like cover types at all sites. Unlike crediting of habitat units (HUs) on other WDFW owned lands, Bonneville Power Administration received full credit for HUs generated on these sites.

  6. HEP@Home - A distributed computing system based on BOINC

    CERN Document Server

    Amorim, A; Andrade, P; Amorim, Antonio; Villate, Jaime; Andrade, Pedro

    2005-01-01

    Project SETI@HOME has proven to be one of the biggest successes of distributed computing during the last years. With a quite simple approach SETI manages to process large volumes of data using a vast amount of distributed computer power. To extend the generic usage of this kind of distributed computing tools, BOINC is being developed. In this paper we propose HEP@HOME, a BOINC version tailored to the specific requirements of the High Energy Physics (HEP) community. The HEP@HOME will be able to process large amounts of data using virtually unlimited computing power, as BOINC does, and it should be able to work according to HEP specifications. In HEP the amounts of data to be analyzed or reconstructed are of central importance. Therefore, one of the design principles of this tool is to avoid data transfer. This will allow scientists to run their analysis applications and taking advantage of a large number of CPUs. This tool also satisfies other important requirements in HEP, namely, security, fault-tolerance an...

  7. Curcumin induced nanoscale CD44 molecular redistribution and antigen-antibody interaction on HepG2 cell surface

    Energy Technology Data Exchange (ETDEWEB)

    Wang Mu [Department of Chemistry, Jinan University, 601 Huangpu Road West, Tianhe District, Guangzhou 510632 (China); Ruan Yuxia [Department of Ophthalmology, The First Affiliated Hospital, Jinan University, 601 Huangpu Road West, Tianhe District, Guangzhou 510632 (China); Xing Xiaobo; Chen Qian; Peng, Yuan [Department of Chemistry, Jinan University, 601 Huangpu Road West, Tianhe District, Guangzhou 510632 (China); Cai Jiye, E-mail: tjycai@jnu.edu.cn [Department of Chemistry, Jinan University, 601 Huangpu Road West, Tianhe District, Guangzhou 510632 (China)

    2011-07-04

    Graphical abstract: Highlights: > In this study, we investigate the changes of CD44 expression and distribution on HepG2 cells after curcumin treatment. > We find curcumin is able to change the morphology and ultrastructure of HepG2 cells. > Curcumin can reduce the expression of CD44 molecules and induce the nanoscale molecular redistribution on cell surface. > The binding force between CD44-modified AFM tip and the HepG2 cell surface decreases after curcumin-treatment. - Abstract: The cell surface glycoprotein CD44 was implicated in the progression, metastasis and apoptosis of certain human tumors. In this study, we used atomic force microscope (AFM) to monitor the effect of curcumin on human hepatocellular carcinoma (HepG2) cell surface nanoscale structure. High-resolution imaging revealed that cell morphology and ultrastructure changed a lot after being treated with curcumin. The membrane average roughness increased (10.88 {+-} 4.62 nm to 129.70 {+-} 43.72 nm) and the expression of CD44 decreased (99.79 {+-} 0.16% to 75.14 {+-} 8.37%). Laser scanning confocal microscope (LSCM) imaging showed that CD44 molecules were located on the cell membrane. The florescence intensity in control group was weaker than that in curcumin treated cells. Most of the binding forces between CD44 antibodies and untreated HepG2 cell membrane were around 120-220 pN. After being incubated with curcumin, the major forces focused on 70-150 pN (10 {mu}M curcumin-treated) and 50-120 pN (20 {mu}M curcumin-treated). These results suggested that, as result of nanoscale molecular redistribution, changes of the cell surface were in response to external treatment of curcumin. The combination of AFM and LSCM could be a powerful method to detect the distribution of cell surface molecules and interactions between molecules and their ligands.

  8. Antiproliferative effects of cinobufacini on human hepatocellular carcinoma HepG2 cells detected by atomic force microscopy.

    Science.gov (United States)

    Wu, Qing; Lin, Wei-Dong; Liao, Guan-Qun; Zhang, Li-Guo; Wen, Shun-Qian; Lin, Jia-Ying

    2015-01-21

    To investigate the antiproliferative activity of cinobufacini on human hepatocellular carcinoma HepG2 cells and the possible mechanism of its action. HepG2 cells were treated with different concentrations of cinobufacini. Cell viability was measured by methylthiazolyl tetrazolium (MTT) assay. Cell cycle distribution was analyzed by flow cytometry (FCM). Cytoskeletal and nuclear alterations were observed by fluorescein isothiocyanate-phalloidin and DAPI staining under a laser scanning confocal microscope. Changes in morphology and ultrastructure of cells were detected by atomic force microscopy (AFM) at the nanoscale level. MTT assay indicated that cinobufacini significantly inhibited the viability of HepG2 cells in a dose-dependent manner. With the concentration of cinobufacini increasing from 0 to 0.10 mg/mL, the cell viability decreased from 74.9% ± 2.7% to 49.41% ± 2.2% and 39.24% ± 2.1% (P nuclei were not damaged seriously. Additionally, high-resolution AFM imaging revealed that cell morphology and ultrastructure changed a lot after treatment with cinobufacini. It appeared as significant shrinkage and deep pores in the cell membrane, with larger particles and a rougher cell surface. Cinobufacini inhibits the viability of HepG2 cells via cytoskeletal destruction and cell membrane toxicity.

  9. Cellular image classification

    CERN Document Server

    Xu, Xiang; Lin, Feng

    2017-01-01

    This book introduces new techniques for cellular image feature extraction, pattern recognition and classification. The authors use the antinuclear antibodies (ANAs) in patient serum as the subjects and the Indirect Immunofluorescence (IIF) technique as the imaging protocol to illustrate the applications of the described methods. Throughout the book, the authors provide evaluations for the proposed methods on two publicly available human epithelial (HEp-2) cell datasets: ICPR2012 dataset from the ICPR'12 HEp-2 cell classification contest and ICIP2013 training dataset from the ICIP'13 Competition on cells classification by fluorescent image analysis. First, the reading of imaging results is significantly influenced by one’s qualification and reading systems, causing high intra- and inter-laboratory variance. The authors present a low-order LP21 fiber mode for optical single cell manipulation and imaging staining patterns of HEp-2 cells. A focused four-lobed mode distribution is stable and effective in optical...

  10. Shillapoo Wildlife Area 2007 Follow-up HEP Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-03-01

    In April and May 2007 the Regional HEP Team (RHT) conducted a follow-up HEP analysis on the Egger (612 acres) and Herzog (210 acres) parcels located at the north end of the Shillapoo Wildlife Area. The Egger and Herzog parcels have been managed with Bonneville Power Administration funds since acquired in 1998 and 2001 respectively. Slightly more than 936 habitat units (936.47) or 1.14 HUs per acre was generated as an outcome of the 2007 follow-up HEP surveys. Results included 1.65 black-capped chickadee HUs, 280.57 great blue heron HUs, 581.45 Canada goose HUs, 40 mallard HUs, and 32.80 mink HUs. Introduction A follow-up Habitat Evaluation Procedures (HEP) (USFWS 1980) analysis was conducted by the Columbia Basin Fish and Wildlife Authority's (CBFWA) Regional HEP Team (RHT) during April and May 2007 to document changes in habitat quality and to determine the number of habitat units (HUs) to credit Bonneville Power Administration (BPA) for providing operation and maintenance (O&M) funds since WDFW acquired the parcels. The 2007 follow-up HEP evaluation was limited to Shillapoo Wildlife Area (SWA) parcels purchased with Bonneville Power Administration funds. D. Budd (pers. comm.) reported WDFW purchased the 612 acre Egger Farms parcel on November 2, 1998 for $1,737,0001 and the 210 acre Herzog acquisition on June 21, 2001 for $500,000 with Memorandum of Agreement funds (BPA and WDFW 1996) as partial fulfillment of BPA's wildlife mitigation obligation for construction of Bonneville and John Day Dams (Rasmussen and Wright 1989). Anticipating the eventual acquisition of the Egger and Herzog properties, WDFW conducted HEP surveys on these lands in 1994 to determine the potential number of habitat units to be credited to BPA. As a result, HEP surveys and habitat unit calculations were completed as much as seven years prior to acquiring the sites. The term 'Shillapoo Wildlife Area' will be used to describe only the Herzog and Egger parcels in this

  11. A batch system for HEP applications on a distributed IaaS cloud

    International Nuclear Information System (INIS)

    Gable, I; Agarwal, A; Anderson, M; Armstrong, P; Fransham, K; Leavett-Brown, D Harris C; Paterson, M; Penfold-Brown, D; Sobie, R J; Vliet, M; Charbonneau, A; Impey, R; Podaima, W

    2011-01-01

    The emergence of academic and commercial Infrastructure-as-a-Service (IaaS) clouds is opening access to new resources for the HEP community. In this paper we will describe a system we have developed for creating a single dynamic batch environment spanning multiple IaaS clouds of different types (e.g. Nimbus, OpenNebula, Amazon EC2). A HEP user interacting with the system submits a job description file with a pointer to their VM image. VM images can either be created by users directly or provided to the users. We have created a new software component called Cloud Scheduler that detects waiting jobs and boots the user VM required on any one of the available cloud resources. As the user VMs appear, they are attached to the job queues of a central Condor job scheduler, the job scheduler then submits the jobs to the VMs. The number of VMs available to the user is expanded and contracted dynamically depending on the number of user jobs. We present the motivation and design of the system with particular emphasis on Cloud Scheduler. We show that the system provides the ability to exploit academic and commercial cloud sites in a transparent fashion.

  12. A batch system for HEP applications on a distributed IaaS cloud

    Science.gov (United States)

    Gable, I.; Agarwal, A.; Anderson, M.; Armstrong, P.; Fransham, K.; Leavett-Brown, D. Harris C.; Paterson, M.; Penfold-Brown, D.; Sobie, R. J.; Vliet, M.; Charbonneau, A.; Impey, R.; Podaima, W.

    2011-12-01

    The emergence of academic and commercial Infrastructure-as-a-Service (IaaS) clouds is opening access to new resources for the HEP community. In this paper we will describe a system we have developed for creating a single dynamic batch environment spanning multiple IaaS clouds of different types (e.g. Nimbus, OpenNebula, Amazon EC2). A HEP user interacting with the system submits a job description file with a pointer to their VM image. VM images can either be created by users directly or provided to the users. We have created a new software component called Cloud Scheduler that detects waiting jobs and boots the user VM required on any one of the available cloud resources. As the user VMs appear, they are attached to the job queues of a central Condor job scheduler, the job scheduler then submits the jobs to the VMs. The number of VMs available to the user is expanded and contracted dynamically depending on the number of user jobs. We present the motivation and design of the system with particular emphasis on Cloud Scheduler. We show that the system provides the ability to exploit academic and commercial cloud sites in a transparent fashion.

  13. THE HERSCHEL AND JCMT GOULD BELT SURVEYS: CONSTRAINING DUST PROPERTIES IN THE PERSEUS B1 CLUMP WITH PACS, SPIRE, AND SCUBA-2

    Energy Technology Data Exchange (ETDEWEB)

    Sadavoy, S. I.; Di Francesco, J.; Johnstone, D.; Fallscheer, C.; Matthews, B. [Department of Physics and Astronomy, University of Victoria, P.O. Box 355, STN CSC, Victoria, BC V8W 3P6 (Canada); Currie, M. J.; Jenness, T. [Joint Astronomy Centre, 660 N. A' ohoku Place, University Park, Hilo, HI 96720 (United States); Drabek, E.; Hatchell, J. [School of Physics, University of Exeter, Stocker Road, Exeter EX4 4QL (United Kingdom); Nutter, D. [School of Physics and Astronomy, Cardiff University, Queen' s Buildings, The Parade, Cardiff CF24 3AA (United Kingdom); Andre, Ph.; Hennemann, M.; Hill, T.; Koenyves, V. [Laboratoire AIM, CEA/DSM-CNRS-Universite Paris Diderot, IRFU/Service d' Astrophysique, Saclay, F-91191 Gif-sur-Yvette (France); Arzoumanian, D. [IAS, CNRS (UMR 8617), Universite Paris-Sud 11, Batiment 121, F-91400 Orsay (France); Benedettini, M. [Istituto di Astrofisica e Planetologia Spaziali, via Fosso del Cavaliere 100, I-00133 Rome (Italy); Bernard, J.-P. [CNRS, IRAP, 9 Av. colonel Roche, BP 44346, F-31028 Toulouse Cedex 4 (France); Duarte-Cabral, A. [Universite de Bordeaux, LAB, UMR 5804, F-33270 Floirac (France); Friesen, R. [Dunlap Institute, University of Toronto, Toronto, ON M5S 3H8 (Canada); Greaves, J. [School of Physics and Astronomy, University of St. Andrews, North Haugh, St. Andrews, Fife KY16 9SS (United Kingdom); Collaboration: JCMT and Herschel Gould Belt Survey teams; and others

    2013-04-20

    We present Herschel observations from the Herschel Gould Belt Survey and SCUBA-2 science verification observations from the JCMT Gould Belt Survey of the B1 clump in the Perseus molecular cloud. We determined the dust emissivity index using four different techniques to combine the Herschel PACS+SPIRE data at 160-500 {mu}m with the SCUBA-2 data at 450 {mu}m and 850 {mu}m. Of our four techniques, we found that the most robust method was filtering out the large-scale emission in the Herschel bands to match the spatial scales recovered by the SCUBA-2 reduction pipeline. Using this method, we find {beta} Almost-Equal-To 2 toward the filament region and moderately dense material and lower {beta} values ({beta} {approx}> 1.6) toward the dense protostellar cores, possibly due to dust grain growth. We find that {beta} and temperature are more robust with the inclusion of the SCUBA-2 data, improving estimates from Herschel data alone by factors of {approx}2 for {beta} and by {approx}40% for temperature. Furthermore, we find core mass differences of {approx}< 30% compared to Herschel-only estimates with an adopted {beta} = 2, highlighting the necessity of long-wavelength submillimeter data for deriving accurate masses of prestellar and protostellar cores.

  14. Encefalopatia hepática: estudo de 50 casos

    Directory of Open Access Journals (Sweden)

    Luiz Antonio de Lima Resende

    1984-12-01

    Full Text Available Os autores apresentam estudo retrospectivo de 50 pacientes com encefalopatia hepática atendidos no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto a partir de 1959 e discutem os aspectos clínicos, laboratoriais e anátomo-patológicos. Expõem os diagnósticos das hepatopatias, os fatores precipitantes dos episódios de coma e pré-coma hepático e enfatizam o papel da gasometria arterial no estabelecimento do diagnóstico.

  15. Java Analysis Studio and the hep.lcd class library

    Energy Technology Data Exchange (ETDEWEB)

    Ronan, M.T.

    2000-02-14

    The Java Analysis Studio and the hep.lcd class library provide a general framework for performing Java-based Linear Collider Detector (LCD) studies. The package is being developed to fully reconstruct 500 GeV to 1.5 TeV e{sup +}e{sup {minus}} annihilation events for analyzing detector options and performance. The current North American LCD reconstruction effort is aimed at comparing different detailed detector models by performing full detector simulation and reconstruction. This paper describes the JAS/hep.lcd distributed analysis framework and some aspects of the reconstruction and analysis object modeling.

  16. Alteraciones hepáticas inducidas por la nutrición parenteral

    OpenAIRE

    J Salas Salvado; A Recaséns Garica

    1993-01-01

    Liver disorders induced by parenteral nutrition Alteraciones hepáticas inducidas por la nutrición parenteral Liver disorders induced by parenteral nutrition Alteraciones hepáticas inducidas por la nutrición parenteral

  17. 78 FR 64927 - Agency Information Collection Activities; Comment Request; High School Equivalency Program (HEP...

    Science.gov (United States)

    2013-10-30

    ... Agency Information Collection Activities; Comment Request; High School Equivalency Program (HEP) Annual... notice will be considered public records. Title of Collection: High School Equivalency Program (HEP... Office of Migrant Education (OME) is collecting information for the High School Equivalency Program (HEP...

  18. Proceedings of workshop on 'future in HEP computing'

    International Nuclear Information System (INIS)

    Karita, Yukio; Amako, Katsuya; Watase, Yoshiyuki

    1993-12-01

    The workshop was held on March 11 and 12, 1993, at the National Laboratory for High Energy Physics (KEK). The large flow from the conventional system centering around large versatile computers to the down-sizing taking distributed processing systems in it is formed, but its destination is not yet seen. As the concrete themes of 'future in HEP computing', problems toward down-sizing and the approach, future perspective of the networks, and adaptation of software engineering and pointing to object were taken up. At the workshop, lectures were given on requirements in HEP computing, possible solutions from Hitachi and Fujitsu, and network computing with work-stations regarding down-sizing and HEP computing; approaches in INS and KEK regarding future computing system in HEP laboratories; user requirement for future network, network service available in 1995-2005, multi-media communication and network protocols regarding future networks; object-oriented approach for software development, OOP for real time data acquisition and accelerator control; ProdiG activities and future of FORTRAN, F90 and HPF regarding OOP and physics, and trends in software development methodology. (K.I.)

  19. Development of 3D integrated circuits for HEP

    International Nuclear Information System (INIS)

    Yarema, R.; Fermilab

    2006-01-01

    Three dimensional integrated circuits are well suited to improving circuit bandwidth and increasing effective circuit density. Recent advances in industry have made 3D integrated circuits an option for HEP. The 3D technology is discussed in this paper and several examples are shown. Design of a 3D demonstrator chip for the ILC is presented

  20. I - Multivariate Classification and Machine Learning in HEP

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    Traditional multivariate methods for classification (Stochastic Gradient Boosted Decision Trees and Multi-Layer Perceptrons) are explained in theory and practise using examples from HEP. General aspects of multivariate classification are discussed, in particular different regularisation techniques. Afterwards, data-driven techniques are introduced and compared to MC-based methods.

  1. A community Q&A for HEP Software and Computing ?

    CERN Multimedia

    CERN. Geneva

    2016-01-01

    How often do you use StackOverflow or ServerFault to find information in your daily work? Would you be interested in a community Q&A site for HEP Software and Computing, for instance a dedicated StackExchange site? I looked into this question...

  2. Habitat Evaluation Procedures (HEP) Report; Beaver Lake, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    On August 14, 2003, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Beaver Lake property, an acquisition completed by the Kalispel Tribe of Indians in November 2002. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Beaver Lake Project provides a total of 232.26 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 136.58 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Forested wetland habitat provides 20.02 HUs for bald eagle, black-caped chickadee, mallard, and white-tailed deer. Scrub-shrub wetland habitat provides 7.67 HUs for mallard, yellow warbler, and white-tailed deer. Grassland meadow provides 22.69 HUs for Canada goose and mallard. Emergent wetlands provide 35.04 HUs for Canada goose, mallard, and muskrat. Open water provided 10.26 HUs for Canada goose, mallard, and muskrat. The objective of using HEP at the Beaver Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  3. Habitat Evaluation Procedures (HEP) Report; Gamblin Lake, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    On August 12, 2003, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Gamblin Lake property, an acquisition completed by the Kalispel Tribe of Indians in December 2002. Evaluation species and appropriate models include bald eagle, black-capped chickadee, mallard, muskrat, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Gamblin Lake Project provides a total of 273.28 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 127.92 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Forested wetland habitat provides 21.06 HUs for bald eagle, black-caped chickadee, mallard, and white-tailed deer. Wet meadow provides 78.05 HUs for Canada goose and mallard. Emergent wetland habitat provides 46.25 HUs for mallard, muskrat, and Canada goose. The objective of using HEP at the Gamblin Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  4. Habitat Evaluation Procedures (HEP) Report; Carey Creek, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    In August 2002, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Carey Creek property, an acquisition completed by the Kalispel Tribe of Indians in December 2001. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Carey Creek Project provides a total of 172.95 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 4.91 HUs for bald eagle, black-capped chickadee, and white-tailed deer. Forested wetlands provide 52.68 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Scrub-shrub wetlands provide 2.82 HUs for mallard, yellow warbler and white-tailed deer. Wet meadow and grassland meadow provide 98.13 HUs for mallard and Canada goose. Emergent wetlands provide 11.53 HUs for mallard, muskrat, and Canada goose. Open water provides 2.88 HUs for Canada goose, mallard, and muskrat. The objective of using HEP at the Carey Creek Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  5. Apoptosis in HEp-2 cells infected with Ureaplasma diversum

    Directory of Open Access Journals (Sweden)

    Aline Teixeira Amorim

    2014-01-01

    Full Text Available BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. RESULTS: The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. CONCLUSIONS: The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS. Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells.

  6. Apoptosis in HEp-2 cells infected with Ureaplasma diversum.

    Science.gov (United States)

    Amorim, Aline Teixeira; Marques, Lucas Miranda; Santos, Angelita Maria Oliveira Gusmão; Martins, Hellen Braga; Barbosa, Maysa Santos; Rezende, Izadora Souza; Andrade, Ewerton Ferraz; Campos, Guilherme Barreto; Lobão, Tássia Neves; Cortez, Beatriz Araujo; Monezi, Telma Alvez; Machado-Santelli, Glaucia Maria; Timenetsky, Jorge

    2014-09-04

    Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location of Ureaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversum invasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and anti-apoptotic gene expression, and FITC Annexin V/Dead Cell Apoptosis Kit. The number of internalized ureaplasma in HEp-2 cells increased significantly throughout the infection. The flow cytometry analysis with fluorochromes to detect membrane depolarization and gene expression for caspase 2, 3 and 9 increased in infected cells after 24 hours. However, after 72 hours a considerable decrease of apoptotic cells was observed. The data suggests that apoptosis may be initially induced by some isolates in association with HEp-2 cells, but over time, there was no evidence of apoptosis in the presence of ureaplasma and HEp-2 cells. The initial increase and then decrease in apoptosis could be related to bacterial pathogen-associated molecular pattern (PAMPS). Moreover, the isolates of U. diversum presented differences in the studied parameters for apoptosis. It was also observed that the amount of microorganisms was not proportional to the induction of apoptosis in HEp-2 cells.

  7. On-demand provisioning of HEP compute resources on cloud sites and shared HPC centers

    Science.gov (United States)

    Erli, G.; Fischer, F.; Fleig, G.; Giffels, M.; Hauth, T.; Quast, G.; Schnepf, M.; Heese, J.; Leppert, K.; Arnaez de Pedro, J.; Sträter, R.

    2017-10-01

    This contribution reports on solutions, experiences and recent developments with the dynamic, on-demand provisioning of remote computing resources for analysis and simulation workflows. Local resources of a physics institute are extended by private and commercial cloud sites, ranging from the inclusion of desktop clusters over institute clusters to HPC centers. Rather than relying on dedicated HEP computing centers, it is nowadays more reasonable and flexible to utilize remote computing capacity via virtualization techniques or container concepts. We report on recent experience from incorporating a remote HPC center (NEMO Cluster, Freiburg University) and resources dynamically requested from the commercial provider 1&1 Internet SE into our intitute’s computing infrastructure. The Freiburg HPC resources are requested via the standard batch system, allowing HPC and HEP applications to be executed simultaneously, such that regular batch jobs run side by side to virtual machines managed via OpenStack [1]. For the inclusion of the 1&1 commercial resources, a Python API and SDK as well as the possibility to upload images were available. Large scale tests prove the capability to serve the scientific use case in the European 1&1 datacenters. The described environment at the Institute of Experimental Nuclear Physics (IEKP) at KIT serves the needs of researchers participating in the CMS and Belle II experiments. In total, resources exceeding half a million CPU hours have been provided by remote sites.

  8. Extreme I/O on HPC for HEP using the Burst Buffer at NERSC

    Science.gov (United States)

    Bhimji, Wahid; Bard, Debbie; Burleigh, Kaylan; Daley, Chris; Farrell, Steve; Fasel, Markus; Friesen, Brian; Gerhardt, Lisa; Liu, Jialin; Nugent, Peter; Paul, Dave; Porter, Jeff; Tsulaia, Vakho

    2017-10-01

    In recent years there has been increasing use of HPC facilities for HEP experiments. This has initially focussed on less I/O intensive workloads such as generator-level or detector simulation. We now demonstrate the efficient running of I/O-heavy analysis workloads on HPC facilities at NERSC, for the ATLAS and ALICE LHC collaborations as well as astronomical image analysis for DESI and BOSS. To do this we exploit a new 900 TB NVRAM-based storage system recently installed at NERSC, termed a Burst Buffer. This is a novel approach to HPC storage that builds on-demand filesystems on all-SSD hardware that is placed on the high-speed network of the new Cori supercomputer. We describe the hardware and software involved in this system, and give an overview of its capabilities, before focusing in detail on how the ATLAS, ALICE and astronomical workflows were adapted to work on this system. We describe these modifications and the resulting performance results, including comparisons to other filesystems. We demonstrate that we can meet the challenging I/O requirements of HEP experiments and scale to many thousands of cores accessing a single shared storage system.

  9. Encefalopatía Hepática

    Directory of Open Access Journals (Sweden)

    Juan Carlos Restrepo Gutiérrez

    2008-12-01

    Full Text Available En pacientes con enfermedad hepática aguda o crónica la encefalopatía hepática es una complicación progresiva y reversible en su mayoría de veces, que puede reducir la sobrevida y la calidad de vida de quienes la presenten. Dentro de su patogénesis existen diferentes hipótesis entre las cuales la más aceptada ha sido la de la neurotoxicidad por amonio, que incluso podría explicar las otras hipótesis planteadas, como la del edema de los astrocitos, la toxicidad por manganeso, entre otras. La clasificación actual de la encefalopatía hepática se propuso en el congreso mundial de Gastroenterología en 1998 y se basa en la naturaleza de la disfunción hepática, duración y características de las manifestaciones neurológicas. Para su diagnostico se requiere de un buen reconocimiento clínico puesto que es principalmente de exclusión; sin embargo actualmente se cuenta con los criterios de West Haven para clasificar la severidad de su presentación. En este síndrome el tratamiento comprende diferentes elementos –como la dieta proteica, disacáridos no absorbibles y terapia antibiótica, que tienen como finalidad la resolución de las manifestaciones y mejorar la calidad de vida de los pacientes, aunque sin dejar de lado que tratar los factores precipitantes son la principal medida terapéutica para la encefalopatía hepática. El objetivo de este artículo es revisar los últimos conceptos de la encefalopatía hepática; con especial énfasis en su patogénesis, clasificación, diagnostico y tratamiento.

  10. Cytotoxicity and apoptotic effects of tea polyphenol-loaded chitosan nanoparticles on human hepatoma HepG2 cells.

    Science.gov (United States)

    Liang, Jin; Li, Feng; Fang, Yong; Yang, Wenjian; An, Xinxin; Zhao, Liyan; Xin, Zhihong; Cao, Lin; Hu, Qiuhui

    2014-03-01

    Tea polyphenols have strong antioxidant and antitumor activities. However, these health benefits are limited due to their poor in vivo stability and low bioavailability. Chitosan nanoparticles as delivery systems may provide an alternative approach for enhancing bioavailability of poorly absorbed drugs. In this study, tea polyphenol-loaded chitosan nanoparticles have been prepared using two different chitosan biomaterials, and their antitumor effects were evaluated in HepG2 cells, including cell cytotoxicity comparison, cell morphology analysis, cell apoptosis and cell cycle detection. The results indicated that the tea polyphenol-loaded chitosan nanoparticles showed a branch shape and heterogeneous distribution in prepared suspension. MTT assay suggested that tea polyphenol-loaded chitosan nanoparticles could inhibit the proliferation of HepG2 cells, and the cytotoxicity rates were increased gradually and appeared an obvious dose-dependent relationship. Transmission electron microscope images showed that the HepG2 cells treated with tea polyphenol-loaded chitosan nanoparticles exhibited some typical apoptotic features, such as microvilli disappearance, margination of nuclear chromatin, intracytoplasmic vacuoles and the mitochondrial swelling. In addition, the tea polyphenol-loaded chitosan nanoparticles had relatively weak inhibitory effects on HepG2 cancer cells compared with tea polyphenols. Tea polyphenols not only induced cancer cell apoptosis, but also promoted their necrosis. However, tea polyphenol-loaded chitosan nanoparticles exhibited their antitumor effects mainly through inducing cell apoptosis. Our results revealed that the inhibition effects of tea polyphenol-loaded chitosan nanoparticles on tumor cells probably depended on their controlled drug release and effective cell delivery. The chitosan nanoparticles themselves as the delivery carrier showed limited antitumor effects compared with their encapsulated drugs. Copyright © 2013. Published by

  11. The HepHIV 2017 Conference in Malta

    DEFF Research Database (Denmark)

    Raben, D; Hoekstra, M; Sperle, I

    2018-01-01

    ) was performed to retrieve the key outcomes of the conference. RESULTS: The key result from the conference was a call to action summarising key priorities in HIV and viral hepatitis testing and linkage to care. This included improving monitoring of viral hepatitis and HIV, mixing testing strategies and ensuring......OBJECTIVES: The objective of the article is to provide an overview of the results of the HepHIV 2017 Conference organized by the HIV in Europe initiative under the Maltese EU Presidency in January 2017. METHODS: A thourough review of all conference presentations (oral and poster presentations...... policy support. The important contribution and outcomes of EU funded projects OptTEST and EuroHIVEdat was highlighted. CONCLUSION: An integrated approach to earlier testing and linkage to care across diseases is needed in Europe and the HepHIV conferences create an important forum to reach this aim....

  12. Towards Provenance and Traceability in CRISTAL for HEP

    Science.gov (United States)

    Shamdasani, Jetendr; Branson, Andrew; McClatchey, Richard

    2014-06-01

    This paper discusses the CRISTAL object lifecycle management system and its use in provenance data management and the traceability of system events. This software was initially used to capture the construction and calibration of the CMS ECAL detector at CERN for later use by physicists in their data analysis. Some further uses of CRISTAL in different projects (CMS, neuGRID and N4U) are presented as examples of its flexible data model. From these examples, applications are drawn for the High Energy Physics domain and some initial ideas for its use in data preservation HEP are outlined in detail in this paper. Currently investigations are underway to gauge the feasibility of using the N4U Analysis Service or a derivative of it to address the requirements of data and analysis logging and provenance capture within the HEP long term data analysis environment.

  13. Towards Provenance and Traceability in CRISTAL for HEP

    CERN Document Server

    Shamdasani, Jetendr; McClatchey, Richard

    2014-01-01

    This paper discusses the CRISTAL object lifecycle management system and its use in provenance data management and the traceability of system events. This software was initially used to capture the construction and calibration of the CMS ECAL detector at CERN for later use by physicists in their data analysis. Some further uses of CRISTAL in different projects (CMS, neuGRID and N4U) are presented as examples of its flexible data model. From these examples, applications are drawn for the High Energy Physics domain and some initial ideas for its use in data preservation HEP are outlined in detail in this paper. Currently investigations are underway to gauge the feasibility of using the N4U Analysis Service or a derivative of it to address the requirements of data and analysis logging and provenance capture within the HEP long term data analysis environment.

  14. Hematoma hepático subcapsular en el puerperio

    OpenAIRE

    Carazo H,Belén; Romero C,Manuel Ángel; Puebla M,Cristina; Sanz M,Amelia; Rojas P,Beatriz

    2013-01-01

    El hematoma hepático subcapsular es una complicación infrecuente y grave durante la gestación o el período puerperal. Esta patología generalmente se relaciona con preeclampsia o síndrome de HELLP. Su diagnóstico debe confirmarse por tomografía axial computarizada. La precocidad del diagnóstico y tratamiento es importante para evitar la ruptura del hematoma.

  15. Future plans for HEP computing in the US

    International Nuclear Information System (INIS)

    Ballam, J.

    1985-06-01

    The computing requirements of the US HEP Community are set forth. These will be dominated in the next five years by the pantip (TEV I) and e + e - (SLC and CESR) experiments. The ensuing period will be almost completely driven by the data generated by the superconducting super collider (SSC). Plans for near term computing are presented along with speculations for the SSC. Brief descriptions of accelerator and theoretical physics plans are also presented

  16. Invasion of Ureaplasma diversum in Hep-2 cells

    OpenAIRE

    Marques, Lucas Miranda; Ueno, Priscilla M; Buzinhani, Melissa; Cortez, Beatriz A; Neto, Renata L; Yamaguti, Maur?cio; Oliveira, Ros?ngela C; Guimar?es, Ana M?rcia S; Monezi, Telma A; Braga, Antonio Carlos R; Machado-Santelli, Gl?ucia M; Timenetsky, Jorge

    2010-01-01

    Abstract Background Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Micros...

  17. Aspects of scientific visualization for HEP analysis at Fermilab

    International Nuclear Information System (INIS)

    Kallenbach, Jeff; Lebrun, Paul

    1996-01-01

    Based on the workshop on scientific visualization held on Aug 7-9, 1995 at Fermilab, and practical experience with IRIS Explorer, we comment on the use of Open GL based for Event Displays and related HEP data analysis. WE wish to compare the pros and cons of such systems on technical grounds, case of use, and most of all, application interfaces, as the programmer and the user are often the same person. Costs and educational considerations will also be briefly discussed. (author)

  18. Curcumin induced nanoscale CD44 molecular redistribution and antigen-antibody interaction on HepG2 cell surface

    International Nuclear Information System (INIS)

    Wang Mu; Ruan Yuxia; Xing Xiaobo; Chen Qian; Peng, Yuan; Cai Jiye

    2011-01-01

    Graphical abstract: Highlights: → In this study, we investigate the changes of CD44 expression and distribution on HepG2 cells after curcumin treatment. → We find curcumin is able to change the morphology and ultrastructure of HepG2 cells. → Curcumin can reduce the expression of CD44 molecules and induce the nanoscale molecular redistribution on cell surface. → The binding force between CD44-modified AFM tip and the HepG2 cell surface decreases after curcumin-treatment. - Abstract: The cell surface glycoprotein CD44 was implicated in the progression, metastasis and apoptosis of certain human tumors. In this study, we used atomic force microscope (AFM) to monitor the effect of curcumin on human hepatocellular carcinoma (HepG2) cell surface nanoscale structure. High-resolution imaging revealed that cell morphology and ultrastructure changed a lot after being treated with curcumin. The membrane average roughness increased (10.88 ± 4.62 nm to 129.70 ± 43.72 nm) and the expression of CD44 decreased (99.79 ± 0.16% to 75.14 ± 8.37%). Laser scanning confocal microscope (LSCM) imaging showed that CD44 molecules were located on the cell membrane. The florescence intensity in control group was weaker than that in curcumin treated cells. Most of the binding forces between CD44 antibodies and untreated HepG2 cell membrane were around 120-220 pN. After being incubated with curcumin, the major forces focused on 70-150 pN (10 μM curcumin-treated) and 50-120 pN (20 μM curcumin-treated). These results suggested that, as result of nanoscale molecular redistribution, changes of the cell surface were in response to external treatment of curcumin. The combination of AFM and LSCM could be a powerful method to detect the distribution of cell surface molecules and interactions between molecules and their ligands.

  19. HEP-FCE Working Group on Libraries and Tools

    Energy Technology Data Exchange (ETDEWEB)

    Borgland, Anders [SLAC National Accelerator Lab., Menlo Park, CA (United States); Elmer, Peter [Princeton Plasma Physics Lab. (PPPL), Princeton, NJ (United States); Kirby, Michael [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States); Patton, Simon [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Potekhin, Maxim [Brookhaven National Lab. (BNL), Upton, NY (United States); Viren, Brett [Brookhaven National Lab. (BNL), Upton, NY (United States); Yanny, Brian [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2014-12-19

    The High-Energy Physics Forum for Computational Excellence (HEP-FCE) was formed by the Department of Energy as a follow-up to a recent report from the Topical Panel on Computing[1] and the associated P5 recommendation[2]. It is a pilot project distributed across the DOE Labs. During this initial incubation period the Forum is to develop a plan for a robust, long-term organization structure and a functioning web presence for forum activities and outreach, and a study of hardware and software needs across the HEP program. In the following sections we give this working group’s “vision” for aspects and qualities we wish to see in a future HEP-FCE. We then give a prioritized list of technical activities with suggested scoping and deliverables that can be expected to provide cross-experiment benefits. The remaining bulk of the report gives a technical survey of some specific “areas of opportunity” for cross-experiment benefit in the realm of software libs/tools. This survey serves as support for the vision and prioritized list. For each area we describe the ways that cross-experiment benefit is achieved today, as well as describe known failings or pitfalls where such benefit has failed to be achieved and which should be avoided in the future. For both cases, we try to give concrete examples. Each area then ends with an examination of what opportunities exist for improvements in that particular area.

  20. HEP Computing Tools, Grid and Supercomputers for Genome Sequencing Studies

    Science.gov (United States)

    De, K.; Klimentov, A.; Maeno, T.; Mashinistov, R.; Novikov, A.; Poyda, A.; Tertychnyy, I.; Wenaus, T.

    2017-10-01

    PanDA - Production and Distributed Analysis Workload Management System has been developed to address ATLAS experiment at LHC data processing and analysis challenges. Recently PanDA has been extended to run HEP scientific applications on Leadership Class Facilities and supercomputers. The success of the projects to use PanDA beyond HEP and Grid has drawn attention from other compute intensive sciences such as bioinformatics. Recent advances of Next Generation Genome Sequencing (NGS) technology led to increasing streams of sequencing data that need to be processed, analysed and made available for bioinformaticians worldwide. Analysis of genomes sequencing data using popular software pipeline PALEOMIX can take a month even running it on the powerful computer resource. In this paper we will describe the adaptation the PALEOMIX pipeline to run it on a distributed computing environment powered by PanDA. To run pipeline we split input files into chunks which are run separately on different nodes as separate inputs for PALEOMIX and finally merge output file, it is very similar to what it done by ATLAS to process and to simulate data. We dramatically decreased the total walltime because of jobs (re)submission automation and brokering within PanDA. Using software tools developed initially for HEP and Grid can reduce payload execution time for Mammoths DNA samples from weeks to days.

  1. Images

    Data.gov (United States)

    National Aeronautics and Space Administration — Images for the website main pages and all configurations. The upload and access points for the other images are: Website Template RSW images BSCW Images HIRENASD...

  2. Selection of scFvs specific for the HepG2 cell line using ribosome ...

    Indian Academy of Sciences (India)

    In order to isolate specific scFvs, recognizing HepG2 negative selection on a normal hepatocyte line WRL-68 was carried out before three rounds of positive selection on HepG2. After three rounds of panning, cell enzyme-linked immunosorbent assay (ELISA) showed that one of the scFvs had high affinity for the HepG2 cell ...

  3. Antioxidant mechanism of mitochondria-targeted plastoquinone SkQ1 is suppressed in aglycemic HepG2 cells dependent on oxidative phosphorylation

    Czech Academy of Sciences Publication Activity Database

    Ježek, J.; Engstová, Hana; Ježek, Petr

    2017-01-01

    Roč. 1858, č. 9 (2017), s. 750-762 ISSN 0005-2728 R&D Projects: GA ČR(CZ) GA17-01813S Institutional support: RVO:67985823 Keywords : mitochondria-targeted antioxidant SkQ1 * mitochondrial Complex I superoxide formation * mitochondrial Complex III superoxide formation * HepG2 cells * NAD(P)H fluorescence lifetime imaging microscopy Subject RIV: ED - Physiology OBOR OECD: Cell biology Impact factor: 4.932, year: 2016

  4. ATLAS@Home: Harnessing Volunteer Computing for HEP

    CERN Document Server

    Cameron, David; The ATLAS collaboration

    2015-01-01

    The ATLAS collaboration has setup a volunteer computing project called ATLAS@home. Volunteers running Monte-Carlo simulation on their personal computer provide significant computing resources, but also belong to a community potentially interested in HEP. Four types of contributors have been identified, whose questions range from advanced technical details to the reason why simulation is needed, how Computing is organized and how it relates to society. The creation of relevant outreach material for simulation, event visualization and distributed production will be described, as well as lessons learned while interacting with the BOINC volunteers community.

  5. Towards an HTTP Ecosystem for HEP Data Access

    International Nuclear Information System (INIS)

    Furano, Fabrizio; Devresse, Adrien; Keeble, Oliver; Hellmich, Martin; Ayllón, Alejandro Álvarez

    2014-01-01

    In this contribution we present a vision for the use of the HTTP protocol for data access and data management in the context of HEP. The evolution of the DPM/LFC software stacks towards a modern framework that can be plugged into Apache servers triggered various initiatives that successfully demonstrated the use of HTTP-based protocols for data access, federation and transfer. This includes the evolution of the FTS3 system towards being able to manage third-party transfers using HTTP. Given the flexibility of the methods, the feature set may also include a subset of the SRM functionality that is relevant to disk systems. The application domain for such an ecosystem of services goes from large scale, Gridlike computing to the data access from laptops, profiting from tools that are shared with the Web community, like browsers, clients libraries and others. Particular focus was put into emphasizing the flexibility of the frameworks, which can interface with a very broad range of components, data stores, catalogues and metadata stores, including the possibility of building high performance dynamic federations of endpoints that build on the fly the feeling of a unique, seamless very efficient system. The overall goal is to leverage standards and standard practices, and use them to provide the higher level functionalities that are needed to fulfil the complex problem of Data Access in HEP. Other points of interest are about harmonizing the possibilities given by the HTTP/WebDAV protocols with existing frameworks like ROOT and already existing Storage Federations based on the XROOTD framework. We also provide quantitative evaluations of the performance that is achievable using HTTP for remote transfer and remote I/O in the context of HEP data. The idea is to contribute the parts that can make possible an ecosystem of services and applications, where the HEP-related features are covered, and the door is open to standard solutions and tools provided by third parties, in the

  6. A review of the possible future HEP Accelerator Projects

    CERN Multimedia

    CERN. Geneva

    2010-01-01

    The review, as already invited for presentation at the recent International Conference on High Energy Physics (ICHEP2010), covers all accelerator projects presently envisaged for HEP in the near and mid-term future. After a brief description of the major motivation, issues and necessary R&D of each project, it specially emphasizes the synergies between the different projects and their R&D issues, pointing out their complementarities and the efficient collaborative approach to address them. It finally summarizes the relative schedule of the various projects in their different phases, pointing out their global interdependence.

  7. HEP graphics: Standards and portability versus performance and cost

    International Nuclear Information System (INIS)

    Myers, D.R.

    1989-01-01

    After mentioning the range of applications for which computer graphics is used in high energy physics, some of the characteristics required of graphics hardware are presented, along with a discussion of how well these requirements are likely to be met by the computer industry. Moving on to the topic of software, the status of the more important graphics standards is covered briefly, as well as their evolution in the medium term. The point will be made that HEP, perhaps even more than other areas, has conflicting requirements for graphics systems. The subject is manpower-intensive, and the physics community has to made a trade-off. (orig.)

  8. Putative identification of components in Zengye Decoction and their effects on glucose consumption and lipogenesis in insulin-induced insulin-resistant HepG2 cells.

    Science.gov (United States)

    Liu, Zhenzhen; Kuang, Wenhua; Xu, Xi; Li, Dandan; Zhu, Wufu; Lan, Zhou; Zhang, Xu

    2018-01-15

    Zengye Decoction (ZYD) is a well-known traditional medicine in China used for treating diseases associated with "Yin deficiency" such as diabetes. However, little information is available on its components, pharmacological effects and underlying mechanisms. This study was designed to identify its active components and evaluate the effects and mechanisms of ZYD on glucose consumption and lipogenesis in insulin-induced insulin-resistant (IR)-HepG2 cells. In this study, 45 compounds of ZYD were putatively identified, in which the iridoid glycosides such as catalpol, aucubin and harpagide were identified as the main components. The insulin-resistant (IR)-HepG2 cell model was established and the effect of ZYD at three doses (0.17, 0.5 and 1.5 μg/mL) on cell growth was evaluated with an IncuCyte™ live-cell imaging system. The effects of ZYD on glucose consumption and uptake were evaluated by glucose consumption and uptake assay. Meanwhile, the effect of ZYD on lipogenesis was investigated in IR-HepG2 cells by oil red O (ORO) staining. Western blot was applied to observe the changes in some of the key factors involved in glucose metabolism and lipogenesis. It was found that the ZYD at a dose of 1.5 μg/mL exhibited an inhibitory activity on IR-HepG2 cell growth. Besides, ZYD at doses of 0.5 and 1.5 μg/mL accelerated the glucose consumption, glucose uptake and reduced the lipogenesis in the IR-HepG2 cells. Western blot studies revealed that ZYD phosphorylated AMP-activated protein kinase α subunits (AMPKα), upregulated hexokinase (HK), phosphorylated acetyl-CoA carboxylase alpha (pACC1) and carnitine palmitoyltransferase 1A (CPT1A) in the IR-HepG2 cells. These results indicate ZYD promotes glucose consumption and uptake, and attenuates lipogenesis in IR-HepG2 cells, which may be involved in activating AMPK and regulating its downstream factors including HK, pACC1 and CPT1A. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Big Data in HEP: A comprehensive use case study

    Science.gov (United States)

    Gutsche, Oliver; Cremonesi, Matteo; Elmer, Peter; Jayatilaka, Bo; Kowalkowski, Jim; Pivarski, Jim; Sehrish, Saba; Mantilla Surez, Cristina; Svyatkovskiy, Alexey; Tran, Nhan

    2017-10-01

    Experimental Particle Physics has been at the forefront of analyzing the worlds largest datasets for decades. The HEP community was the first to develop suitable software and computing tools for this task. In recent times, new toolkits and systems collectively called Big Data technologies have emerged to support the analysis of Petabyte and Exabyte datasets in industry. While the principles of data analysis in HEP have not changed (filtering and transforming experiment-specific data formats), these new technologies use different approaches and promise a fresh look at analysis of very large datasets and could potentially reduce the time-to-physics with increased interactivity. In this talk, we present an active LHC Run 2 analysis, searching for dark matter with the CMS detector, as a testbed for Big Data technologies. We directly compare the traditional NTuple-based analysis with an equivalent analysis using Apache Spark on the Hadoop ecosystem and beyond. In both cases, we start the analysis with the official experiment data formats and produce publication physics plots. We will discuss advantages and disadvantages of each approach and give an outlook on further studies needed.

  10. Invasion of Ureaplasma diversum in Hep-2 cells

    Directory of Open Access Journals (Sweden)

    Braga Antonio

    2010-03-01

    Full Text Available Abstract Background Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay. Results The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma. Conclusions The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence.

  11. Invasion of Ureaplasma diversum in Hep-2 cells.

    Science.gov (United States)

    Marques, Lucas Miranda; Ueno, Priscilla M; Buzinhani, Melissa; Cortez, Beatriz A; Neto, Renata L; Yamaguti, Maurício; Oliveira, Rosângela C; Guimarães, Ana Márcia S; Monezi, Telma A; Braga, Antonio Carlos R; Machado-Santelli, Gláucia M; Timenetsky, Jorge

    2010-03-17

    Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma. The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence.

  12. Optimization of HEP Analysis Activities Using a Tier2 Infrastructure

    International Nuclear Information System (INIS)

    Arezzini, S; Bagliesi, G; Boccali, T; Ciampa, A; Mazzoni, E; Coscetti, S; Sarkar, S; Taneja, S

    2012-01-01

    While the model for a Tier2 is well understood and implemented within the HEP Community, a refined design for Analysis specific sites has not been agreed upon as clearly. We aim to describe the solutions adopted at the INFN Pisa, the biggest Tier2 in the Italian HEP Community. A Standard Tier2 infrastructure is optimized for Grid CPU and Storage access, while a more interactive oriented use of the resources is beneficial to the final data analysis step. In this step, POSIX file storage access is easier for the average physicist, and has to be provided in a real or emulated way. Modern analysis techniques use advanced statistical tools (like RooFit and RooStat), which can make use of multi core systems. The infrastructure has to provide or create on demand computing nodes with many cores available, above the existing and less elastic Tier2 flat CPU infrastructure. At last, the users do not want to have to deal with data placement policies at the various sites, and hence a transparent WAN file access, again with a POSIX layer, must be provided, making use of the soon-to-be-installed 10 Gbit/s regional lines. Even if standalone systems with such features are possible and exist, the implementation of an Analysis site as a virtual layer over an existing Tier2 requires novel solutions; the ones used in Pisa are described here.

  13. Applying deep neural networks to HEP job classification

    International Nuclear Information System (INIS)

    Wang, L; Shi, J; Yan, X

    2015-01-01

    The cluster of IHEP computing center is a middle-sized computing system which provides 10 thousands CPU cores, 5 PB disk storage, and 40 GB/s IO throughput. Its 1000+ users come from a variety of HEP experiments. In such a system, job classification is an indispensable task. Although experienced administrator can classify a HEP job by its IO pattern, it is unpractical to classify millions of jobs manually. We present how to solve this problem with deep neural networks in a supervised learning way. Firstly, we built a training data set of 320K samples by an IO pattern collection agent and a semi-automatic process of sample labelling. Then we implemented and trained DNNs models with Torch. During the process of model training, several meta-parameters was tuned with cross-validations. Test results show that a 5- hidden-layer DNNs model achieves 96% precision on the classification task. By comparison, it outperforms a linear model by 8% precision. (paper)

  14. Avaliação dos tumores hepáticos ao Doppler Doppler evaluation of liver tumors

    Directory of Open Access Journals (Sweden)

    Márcio Martins Machado

    2004-10-01

    Full Text Available Os avanços recentes na ultra-sonografia têm ampliado a possibilidade de detecção de tumores hepáticos. Isto tem auxiliado na perspectiva de melhora do prognóstico destes pacientes, à medida que novas técnicas terapêuticas têm surgido. Neste artigo os autores relatam achados ao Doppler que podem auxiliar na identificação e caracterização dos tumores hepáticos, avaliando dados do Doppler colorido, pulsado e do Doppler de amplitude ("power Doppler". Fazem, também, referência a novas modalidades de imagem, como o uso da harmônica.Recent advances in ultrasound have optimized the detection of liver tumors and helped to improve the prognosis of patients with this condition as newly developed and improved therapeutic modalities have been established. The authors review important Doppler findings which may help in the identification and characterization of some hepatic tumors through the evaluation of color Doppler, pulsed Doppler and power Doppler features. New imaging methods such as the use of harmonics imaging are also reviewed.

  15. Suppressing Pitx2 inhibits proliferation and promotes differentiation of iHepSCs.

    Science.gov (United States)

    Chen, Fei; Yao, Hao; Wang, Minjun; Yu, Bing; Liu, Qinggui; Li, Jianxiu; He, Zhiying; Hu, Yi-Ping

    2016-11-01

    Induced hepatic stem cells (iHepSCs) have great potential as donors for liver cell therapy due to their abilities for self-renewal and bi-potential differentiation. However, the molecular mechanism regulating proliferation and differentiation of iHepSCs is poorly understood. In this study, we provide evidence that the homeodomain transcription factor, Pitx2, is essential to maintain iHepSCs stem cell characteristics. Suppressing Pitx2 expression in iHepSCs by lentivirus mediated specific shRNA markedly reduced the expression of the hepatic stem cell-associated genes (Lgr5, EpCAM, and Sox9) with concomitant inhibition of proliferation by blocking the G1/S phase transition, and these phenotypic changes were reversed upon re-expression of Pitx2. Pitx2 knockdown also resulted in up-regulation of the p53-induced Cdk inhibitor p21, and down-regulation of its downstream effector CDK2-Cyclin E kinase complex. Furthermore, we observed that iHepSCs were more efficiently induced to differentiate into both hepatocytes and cholangiocytes when Pitx2 expression was suppressed, as compared to unmanipulated iHepSCs. These findings reveal that Pitx2 expression may be leveraged to control the status of iHepSCs during expansion in vitro to provide a strategy for further application of iHepSCs in liver cell therapy. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Reduction of angiocidin contributes to decreased HepG2 cell ...

    African Journals Online (AJOL)

    Angiocidin protein production in HepG2 cells were reduced significantly by siRNA. When HepG2 cells were transfected with siRNA-angiocidin, these cells showed very low proliferation activity compared with control cells. Our study suggests that reduction of angiocidin may contribute to decreased proliferation activity in liver ...

  17. Evaluation of the KEMRI Hep-cell II test kit for detection of hepatitis B ...

    African Journals Online (AJOL)

    To evaluate the Hep-cell II test, blood samples were collected from blood donors and processed for detection of HBsAg using Hep-cell II based on the test principle and procedure outlined by the manufacturer. ELISA Axsym HBsAg test was used as golden standard. Of the 400 samples tested, 287 (71.8%) were positive by ...

  18. Selection of scFvs specific for the HepG2 cell line using ribosome ...

    Indian Academy of Sciences (India)

    Madhsudhan

    was transcribed and translated in vitro using a rabbit reticulocyte lysate system. In order to isolate specific scFvs, recognizing HepG2 negative selection on a normal hepatocyte line WRL-68 was carried out before three rounds of positive selection on HepG2. After three rounds of panning, cell enzyme-linked immunosorbent ...

  19. Phenotypic assay of adherent E. coli strains using hep-2 cells on ...

    African Journals Online (AJOL)

    In this paired case-control study of children with diarrhea in Rivers state, the association between HEp-2–adherent Escherichia coli strains and diarrhea was examined. Escherichia coli isolates from stool specimens of children with diarrhea were matched with controls and tested in HEp-2 cell adherence assay. A total of 266 ...

  20. Participants to the 3rd HEP Information Resources Summit, 6-7 May 2009

    CERN Multimedia

    Fermilab, Photo Service

    2009-01-01

    The broad theme of the 3rd HEP Information Resources Summit was "Collaboration between Information Services." As HEP increasingly borders fields such as instrumentation and astrophysics, it was discussed what potential interrelationships and communication this group have to serve this broader research community seamlessly.

  1. Complicações hepáticas na doença falciforme

    OpenAIRE

    Traina,Fabíola; Saad,Sara T. O.

    2007-01-01

    Doentes falciformes podem apresentar alterações hepáticas agudas ou crônicas. As agudas são caracterizadas por dor no quadrante superior direito e icterícia. O diagnóstico diferencial inclui crise aguda de falcização hepática, seqüestro hepático, colestase intra-hepática, colelitíase, coledocolitíase, colecistite e hepatite viral aguda. Estas complicações devem ser diagnosticadas precocemente, através de história clínica, testes de função hepática e exames radiológicos, e o tratamento deve se...

  2. Leptoquark Single and Pair production at LHC with CalcHEP/CompHEP in the complete model

    CERN Document Server

    Belyaev, A; Mehdiyev, R; Pukhov, A E

    2005-01-01

    We study combined leptoquark (LQ) single and pair production at LHC at the level of detector simulation. A set of kinematical cuts to maximize significance for combined signal events has been worked out. It was shown that combination of signatures from LQ single and pair production not only significantly increases the LHC reach, but also allows us to give the correct signal interpretation. In particular, it was found that the LHC has potential to discover LQ with a mass up to 1.2 TeV and 1.5 TeV for the case of scalar and vector LQ, respectively, and LQ single production contributes about 30-50% to the total signal rate for LQ-l-q coupling, taken equal to the electromagnetic coupling. This work is based on the implementation of the most general form of scalar and vector LQ interactions with quarks and gluons into CalcHEP/CompHEP packages. This implementation, which authors made publicly available, was one the most important aspects of the study.

  3. Polyethyleneimine-coated quantum dots for miRNA delivery and its enhanced suppression in HepG2 cells

    Directory of Open Access Journals (Sweden)

    Liang G

    2016-11-01

    Full Text Available Gaofeng Liang,1 Yang Li,1 Wenpo Feng,1 Xinshuai Wang,2 Aihua Jing,1 Jinghua Li,1 Kaiwang Ma1 1Department of Biomedical Engineering, School of Medical Technology & Engineering, 2Department of Oncology, The First Affiliated Hospital, Henan University of Science & Technology, Luoyang, People’s Republic of China Abstract: Quantum dots (QDs have been intensively investigated for bioimaging, drug delivery, and labeling probes because of their unique optical properties. In this study, CdSe/ZnS QDs-based nonviral vectors with the dual functions of delivering miR-26a plasmid and bioimaging were formulated by capping the surface of CdSe/ZnS QDs with polyethyleneimine (PEI. The PEI-coated QDs were capable of condensing miR-26a expression vector into nanocomplexes that can emit strong red luminescence when loaded with CdSe/ZnS QDs. Further results showed that PEI-modified nanoparticles (NPs could transfect miR-26a plasmid into HepG2 cells in vitro. Meanwhile, imaging of living cells could be achieved based on the CdSe/ZnS QDs. Further study suggested that miR-26a transfection up-regulated miR-26a expression, induced cycle arrest, and triggered proliferation inhibition in HepG2 cells. The results indicated that PEI-coated QD NPs possess the capability of bioimaging and gene delivery and could be a promising vehicle with the engineering of QD NPs for gene therapy in the future. Keywords: miR-26a, PEI/QDs, HepG2, gene delivery, bioimaging

  4. GSDC: A Unique Data Center in Korea for HEP research

    Directory of Open Access Journals (Sweden)

    Ahn Sang-Un

    2017-01-01

    Full Text Available Global Science experimental Data hub Center (GSDC at Korea Institute of Science and Technology Information (KISTI is a unique data center in South Korea established for promoting the fundamental research fields by supporting them with the expertise on Information and Communication Technology (ICT and the infrastructure for High Performance Computing (HPC, High Throughput Computing (HTC and Networking. GSDC has supported various research fields in South Korea dealing with the large scale of data, e.g. RENO experiment for neutrino research, LIGO experiment for gravitational wave detection, Genome sequencing project for bio-medical, and HEP experiments such as CDF at FNAL, Belle at KEK, and STAR at BNL. In particular, GSDC has run a Tier-1 center for ALICE experiment using the LHC at CERN since 2013. In this talk, we present the overview on computing infrastructure that GSDC runs for the research fields and we discuss on the data center infrastructure management system deployed at GSDC.

  5. Accessing HEP Collaboration documents using WWW and WAIS

    International Nuclear Information System (INIS)

    Nguyen, T.D.; Buckley-Geer, E.; Ritchie, D.J.

    1995-09-01

    WAIS stands for Wide Area Information Server. It is a distributed information retrieval system. A WAIS system has a client-server architecture which consists of clients talking to a server via a TCP/IP network using the ANSI standard Z39-50 VI protocol. A freely available version (FreeWAIS) is supported by the Clearinghouse for Networked Information Discovery and Retrieval, also known as CNIDR. FreeWAIS-sf, which is the software the authors are using at Fermilab, is an extension of FreeWAIS. FreeWAIS-sf supports all the functionalities which FreeWAIS offers as well as additional indexing and searching capabilities for structured fields. World Wide Web (WWW) was originally developed by Tim Berners-Lee at CERN and is now the backbone for serving information on Internet. Here, the authors describe a system for accessing HEP collaboration documents using WWW and WAIS

  6. Unbundling of HEP's core activities and the electricity price

    International Nuclear Information System (INIS)

    Jurisic, S.-L.; Hamidovic-MAtonickin, K.

    2000-01-01

    At the height of proposals and discussions with regard to unbundling of the core business activities of the electricity industry: electricity generation, transmission and distribution, the unbundling conditions and their impact on electricity prices are rarely mentioned. The purpose and the objective of the electricity market liberalisation and restructuring of HEP should be a lower electricity price at the doorstep of the final costumers. Based on earlier analyses it can be said that the average cost of the core activities, i.e. electricity generation, transmission and distribution, is above the average electricity sale price to final customers in the tariff system. Hereinafter it will be shown how much electricity costs and how the electricity price is formed. (author)

  7. Measurements of the {sup 6}He+p resonant scattering

    Energy Technology Data Exchange (ETDEWEB)

    Condori, R. Pampa; Lichtenthäler, R.; Lépine-Szily, A.; Gasques, L. R.; Morais, M. C.; Scarduelli, V. B.; Leistenschneider, E.; Alcántara-Núñez, J. A. [Departamento de Física Nuclear, Instituto de Física da Universidade de São Paulo, P.O. Box 66318, SP (Brazil); Faria, P. N. de; Mendes Jr, D. R. [Universidade Federal Fluminense, Centro de Estudos Gerais, Instituto de Física, Niteroi, RJ (Brazil); Pires, K. C. C. [Universidade Tecnológica Federal do Paraná, Campus Cornélio Procópio, PR (Brazil); Shorto, J. M. B. [Instituto de Pesquisas Energeticas e Nucleares, IPEN/CNEN, São Paulo (Brazil); Assunção, M. [Universidade Federal de São Paulo, Campus Diadema, São Paulo, SP (Brazil)

    2014-11-11

    Measurements of the p({sup 6}He,p) elastic scattering excitation function have been performed in the RIBRAS system using a {sup 6}He secondary beam and a CH{sub 2} polyethylene thick target. The motivation is to observe states of the compound nucleus {sup 7}Li in the excitation energy range of E{sub exc}{sup 7Li} = 10.8-11.8MeV, where the isobaric analog state of {sup 7}He ground state lies. Excitation functions have been obtained at three laboratory angles θ{sub lab} = 0°, 20°, and 25° which correspond to θ{sub c.m} = 180°, 140°, and 130°.

  8. Microelectronics and nanoelectronics trends, and applications to HEP instrumentation

    CERN Multimedia

    CERN. Geneva

    2004-01-01

    Lecture 1 : Microelectronics and HEP instrumentation CMOS technology has been the leading technology in microelectronics for more that 30 years thanks to its outstanding capability to miniaturization and low power consumption. A brief history of the microelectronics semiconductor industry is presented with applications for LEP and LHC experiments. Lecture 2: Future trends in microelectronics and nanoelectronics Trends in miniaturization point to the fabrication of ULSI nanoscale CMOS circuits by the end of the decade. Device issues and quantum effects in nanoscale MOS transistor will be discussed. Beyond CMOS technology, several technology avenues based on nanotechnology are under investigation. We will present some promising nanoelectronic devices and circuits based on Single Electron Tunneling (SET) transistor, nanowire, quantum dot and carbon nanotubes. Lecture 3: Monolithic pixel detectors Microvertex detectors for particle physics experiments currently uses hybrid silicon pixel detector. Novel emerging m...

  9. ATLAS@Home: Harnessing Volunteer Computing for HEP

    CERN Document Server

    Bourdarios, Claire; Filipcic, Andrej; Lancon, Eric; Wu, Wenjing

    2015-01-01

    A recent common theme among HEP computing is exploitation of opportunistic resources in order to provide the maximum statistics possible for Monte-Carlo simulation. Volunteer computing has been used over the last few years in many other scientific fields and by CERN itself to run simulations of the LHC beams. The ATLAS@Home project was started to allow volunteers to run simulations of collisions in the ATLAS detector. So far many thousands of members of the public have signed up to contribute their spare CPU cycles for ATLAS, and there is potential for volunteer computing to provide a significant fraction of ATLAS computing resources. Here we describe the design of the project, the lessons learned so far and the future plans.

  10. HEP Outreach, Inreach, and Web 2.0

    Science.gov (United States)

    Goldfarb, Steven

    2011-12-01

    I report on current usage of multimedia and social networking "Web 2.0" tools for Education and Outreach in high-energy physics, and discuss their potential for internal communication within large worldwide collaborations, such as those of the LHC. Following a brief description of the history of Web 2.0 development, I present a survey of the most popular sites and describe their usage in HEP to disseminate information to students and the general public. I then discuss the potential of certain specific tools, such as document and multimedia sharing sites, for boosting the speed and effectiveness of information exchange within the collaborations. I conclude with a brief discussion of the successes and failures of these tools, and make suggestions for improved usage in the future.

  11. HEP Outreach, Inreach, and Web 2.0

    International Nuclear Information System (INIS)

    Goldfarb, Steven

    2011-01-01

    I report on current usage of multimedia and social networking 'Web 2.0' tools for Education and Outreach in high-energy physics, and discuss their potential for internal communication within large worldwide collaborations, such as those of the LHC. Following a brief description of the history of Web 2.0 development, I present a survey of the most popular sites and describe their usage in HEP to disseminate information to students and the general public. I then discuss the potential of certain specific tools, such as document and multimedia sharing sites, for boosting the speed and effectiveness of information exchange within the collaborations. I conclude with a brief discussion of the successes and failures of these tools, and make suggestions for improved usage in the future.

  12. GSDC: A Unique Data Center in Korea for HEP research

    Science.gov (United States)

    Ahn, Sang-Un

    2017-04-01

    Global Science experimental Data hub Center (GSDC) at Korea Institute of Science and Technology Information (KISTI) is a unique data center in South Korea established for promoting the fundamental research fields by supporting them with the expertise on Information and Communication Technology (ICT) and the infrastructure for High Performance Computing (HPC), High Throughput Computing (HTC) and Networking. GSDC has supported various research fields in South Korea dealing with the large scale of data, e.g. RENO experiment for neutrino research, LIGO experiment for gravitational wave detection, Genome sequencing project for bio-medical, and HEP experiments such as CDF at FNAL, Belle at KEK, and STAR at BNL. In particular, GSDC has run a Tier-1 center for ALICE experiment using the LHC at CERN since 2013. In this talk, we present the overview on computing infrastructure that GSDC runs for the research fields and we discuss on the data center infrastructure management system deployed at GSDC.

  13. Offering Global Collaboration Services beyond CERN and HEP

    CERN Document Server

    Fernandes, J; Baron, T

    2015-01-01

    The CERN IT department has built over the years a performant and integrated ecosystem of collaboration tools, from videoconference and webcast services to event management software. These services have been designed and evolved in very close collaboration with the various communities surrounding the laboratory and have been massively adopted by CERN users. To cope with this very heavy usage, global infrastructures have been deployed which take full advantage of CERN's international and global nature. If these services and tools are instrumental in enabling the worldwide collaboration which generates major HEP breakthroughs, they would certainly also benefit other sectors of science in which globalization has already taken place. Some of these services are driven by commercial software (Vidyo or Wowza for example), some others have been developed internally and have already been made available to the world as Open Source Software in line with CERN's spirit and mission. Indico for example is now installed in 10...

  14. Hepatic angiosarcoma: Presentation of two cases Angiosarcoma hepático: Presentación de dos casos

    Directory of Open Access Journals (Sweden)

    J. Egea Valenzuela

    2009-06-01

    Full Text Available Hepatic angiosarcoma is a rare primary tumor of the liver with a mesenchymal origin. Diagnosis is difficult because clinical manifestations and imaging studies are inconclusive. In many cases a diagnosis is obtained during necropsy, not being apparent during the course of disease. It is associated with several risk factors, but these contribute to explaining only a few of all reported cases. When clinical manifestations begin progression is often fast, and possibilities for curative treatment are limited. We report two cases of hepatic angiosarcoma. In the first one, our patient had an insidious initial course, and then suddenly presented with hepatic failure followed by acute respiratory distress. A diagnosis was reached during necropsy. In the second case, we initiated the study of a chronic liver disease using fine-needle aspiration biopsy, which showed findings suggestive of hepatic angiosarcoma. In the following weeks the patient started on a torpid clinic course, and died from multiple organ failure.El angiosarcoma hepático es una neoplasia de estirpe mesenquimal de baja frecuencia y difícil diagnóstico por su forma inespecífica de manifestarse clínica y radiológicamente. Tanto es así que muchos diagnósticos se obtienen mediante necropsia, no siendo posible poner de manifiesto la enfermedad durante su curso. Se asocia a diferentes agentes etiológicos, pero en la mayoría de los casos no es posible establecer una exposición concreta a ninguno de ellos. Cuando comienza a manifestarse, la evolución suele ser rápida y las opciones de tratamiento curativo son escasas. Presentamos en nuestro trabajo dos casos de angiosarcoma hepático. En el primero, el paciente sufre en principio una evolución insidiosa, presentando al fin, y de forma abrupta, un cuadro de insuficiencia hepática seguido de distrés respiratorio, falleciendo por este motivo. El diagnóstico se alcanza en la necropsia. En el segundo caso se inicia un estudio de

  15. Effects of hydrogen peroxide and apolipoprotein E isoforms on apolipoprotein E trafficking in HepG2 cells.

    Science.gov (United States)

    Sabaretnam, Tharani; Harris, Matthew J; Kockx, Maaike; Witting, Paul K; Le Couteur, David G; Kritharides, Leonard

    2009-12-01

    1. The major source of apolipoprotein E (apoE) is the liver. In the present study, the effects of oxidative stress and apoE isoforms on apoE distribution and trafficking were established using the HepG2 liver tumour cell line. 2. Hydrogen peroxide (0, 25, 250 and 1000 micromol/L) was associated with rapid and concentration-dependent redistribution of apoE into the early endosomal compartment. This redistribution was achieved with a much lower concentration (25 micromol/L) than that needed to induce changes in intracellular apoE mRNA expression, apoE protein levels and markers of oxidative stress (250-1000 micromol/L). 3. Live cell imaging of apoE3-green fluorescent protein revealed a significant decrease in traffic velocity in response to oxidative stress. 4. The E4 isoform was associated with reduced trafficking velocity compared with the E3 isoform under basal conditions. 5. The results indicate that oxidative stress and apoE isoforms influence apoE trafficking and distribution within HepG2 cells. Altered apoE hepatocyte trafficking may provide a mechanistic link between oxidative stress, ageing and some diseases in older people.

  16. Encefalopatía hepática en perros y gatos

    OpenAIRE

    Pellegrino, Fernando Carlos

    2009-01-01

    La encefalopatía hepática (EH) en perros y gatos es un disturbio metabólico complejo del sistema nervioso central que puede deberse a insufi ciencia hepática, defi ciencias enzimáticas en el ciclo de la urea o a desvíos portosistémicos. Como resultado, las funciones hepáticas de desintoxicación se alteran y/o son eludidas, y los constituyentes inalterados de la sangre portal penetran directamente a la circulación sistémica. La patogénesis de la encefalopatía asociada a la insu...

  17. Biomarcadores para fibrosis hepática, avances, ventajas y desventajas

    OpenAIRE

    A. Cequera; M.C. García de León Méndez

    2014-01-01

    La cirrosis hepática en México es una de las principales causas de muerte en sujetos entre los 25 y los 50 años de edad. Una de las principales razones del fracaso terapéutico es el desconocimiento de los mecanismos moleculares que producen el trastorno hepático y lo hacen irreversible. Entre sus características anatómicas prevalece un depósito excesivo de tejido fibroso que adopta diferentes formas, dependiendo de la etiología y etapa de la enfermedad. La biopsia hepática, considerada tra...

  18. Evaluating the scalability of HEP software and multi-core hardware

    International Nuclear Information System (INIS)

    Jarp, Sverre; Lazzaro, Alfio; Leduc, Julien; Nowak, Andrzej

    2011-01-01

    As researchers have reached the practical limits of processor performance improvements by frequency scaling, it is clear that the future of computing lies in the effective utilization of parallel and multi-core architectures. Since this significant change in computing is well underway, it is vital for HEP programmers to understand the scalability of their software on modern hardware and the opportunities for potential improvements. This work aims to quantify the benefit of new mainstream architectures to the HEP community through practical benchmarking on recent hardware solutions, including the usage of parallelized HEP applications.

  19. Estudio del estado bucodental del paciente trasplantado hepático

    OpenAIRE

    Díaz Ortiz, María Luisa; Micó Llorens, José María; Gargallo Albiol, Jordi; Baliellas Comellas, Carmen; Berini Aytés, Leonardo

    2005-01-01

    Desde que se realizó en España el primer trasplante hepático en el año 1984 los avances en la técnica quirúrgica y en los fármacos inmunosupresores empleados han producido un aumento en el número de pacientes trasplantados. El objetivo del presente estudio fue valorar el estado bucodental de los pacientes trasplantados hepáticos. Se realizó un estudio descriptivo transversal de una muestra de pacientes que habían sido sometidos a un trasplante hepático en el Hospital Prín...

  20. UARS PEM Level 2 HEPS B V001 (UARPE2HEPSB) at GES DISC

    Data.gov (United States)

    National Aeronautics and Space Administration — The Particle Environment Monitor (PEM) level 2 High-Energy Particle Spectrometer (HEPS) B daily product contains the electron high-resolution spectral data converted...

  1. Cultivation of HepG2.2.15 on Cytodex-3

    DEFF Research Database (Denmark)

    Lupberger, Joachim; Mund, Andreas; Kock, Josef

    2006-01-01

    BACKGROUND/AIMS: Several novel systems are available to study human hepatitis B virus (HBV) replication in cell culture demanding for efficient cell culture based systems for HBV production. The aim was to enhance HBV production of the HBV stably producing cell line HepG2.2.15 by cultivation...... on spherical micro substrate. METHODS: HepG2.2.15 was cultivated on microcarrier substrate Cytodex-3. HBV specific transcripts, viral protein and genome secretion, cell proliferation and MAP kinase signaling were analyzed. Infectivity of HBV particles was analyzed using primary tupaia hepatocytes. RESULTS......: Compared to stationary flask cultures, HepG2.2.15 on Cytodex-3 secreted 18-fold more HBV genomes, more HBeAg per culture volume and less HBV surface antigen per extracellular viral genome equivalent. This was reflected by a significantly higher infectivity of supernatant derived from carrier grown HepG.2...

  2. UARS PEM Level 2 HEPS A V002 (UARPE2HEPSA) at GES DISC

    Data.gov (United States)

    National Aeronautics and Space Administration — The Particle Environment Monitor (PEM) level 2 High-Energy Particle Spectrometer (HEPS) A daily product contains electron high-resolution spectral data converted to...

  3. HappyFace as a generic monitoring tool for HEP experiments

    Science.gov (United States)

    Kawamura, Gen; Magradze, Erekle; Musheghyan, Haykuhi; Quadt, Arnulf; Rzehorz, Gerhard

    2015-12-01

    The importance of monitoring on HEP grid computing systems is growing due to a significant increase in their complexity. Computer scientists and administrators have been studying and building effective ways to gather information on and clarify a status of each local grid infrastructure. The HappyFace project aims at making the above-mentioned workflow possible. It aggregates, processes and stores the information and the status of different HEP monitoring resources into the common database of HappyFace. The system displays the information and the status through a single interface. However, this model of HappyFace relied on the monitoring resources which are always under development in the HEP experiments. Consequently, HappyFace needed to have direct access methods to the grid application and grid service layers in the different HEP grid systems. To cope with this issue, we use a reliable HEP software repository, the CernVM File System. We propose a new implementation and an architecture of HappyFace, the so-called grid-enabled HappyFace. It allows its basic framework to connect directly to the grid user applications and the grid collective services, without involving the monitoring resources in the HEP grid systems. This approach gives HappyFace several advantages: Portability, to provide an independent and generic monitoring system among the HEP grid systems. Eunctionality, to allow users to perform various diagnostic tools in the individual HEP grid systems and grid sites. Elexibility, to make HappyFace beneficial and open for the various distributed grid computing environments. Different grid-enabled modules, to connect to the Ganga job monitoring system and to check the performance of grid transfers among the grid sites, have been implemented. The new HappyFace system has been successfully integrated and now it displays the information and the status of both the monitoring resources and the direct access to the grid user applications and the grid collective

  4. Cytotoxicity and Antioxidant Activity of Zingiber Officinale and 6-Gingerol on HepG2 Cells

    OpenAIRE

    Hanif, Harliansyah; Azian Murad, Noor; Wan Ngah, Wan Zurinah; Anum Mohd Yusof, Yasmin

    2008-01-01

    The present study was designed to compare the effects of ethanolic extract of ginger (Zingiber officinale) and its phenolic component [6]-Gingerol on viability, antiproliferation and apoptotic levels of human hepatoma cell lines (HepG2) and its antioxidant activity. HepG2 cells were cultured in Eagles minimum essential medium (EMEM) and the percentage of cell cytotoxicity was evaluated by tetrazolium salt (MTS) assay. Antiproliferation and apoptotic levels were measured by 5Bromo-2deoxyuridin...

  5. Habitat Evaluation Procedures (HEP) Report : Grand Coulee Dam Mitigation, 1996-1999 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Kieffer, B.; Singer, Kelly; Abrahamson, Twa-le

    1999-07-01

    The purpose of this Habitat Evaluation Procedures (HEP) study was to determine baseline habitat units and to estimate future habitat units for Bonneville Power Administration (BPA) mitigation projects on the Spokane Indian Reservation. The mitigation between BPA and the Spokane Tribe of Indians (STOI) is for wildlife habitat losses on account of the construction of Grand Coulee Dam. Analysis of the HEP survey data will assist in mitigation crediting and appropriate management of the mitigation lands.

  6. Hemangioma hepático gigante versus hemangioma hepático convencional: características clínicas, factores de riesgo y manejo

    OpenAIRE

    Moctezuma-Velázquez, C.; López-Arce, G.; Martínez-Rodríguez, L.A.; Escalona-Huerta, C.; Chapa-Ibargüengoitia, M.; Torre, A.

    2014-01-01

    Antecedentes: Los hemangiomas hepáticos gigantes (HHG) son aquellos con un tamaño superior a 4 cm. Objetivo: El objetivo de este estudio fue describir los hallazgos clínicos del HHG, sus factores de riesgo, el abordaje diagnóstico y el manejo, y comparar esta información con la del hemangioma convencional. Métodos: Se realizó un análisis retrospectivo del periodo 1990-2008 de pacientes con diagnóstico, sea por imagen o histopatología, de hemangioma hepático atendidos en nuestro hospital...

  7. Revealing the ISM in high redshift starburst galaxies: An analysis of Herschel PACS and SPIRE FTS spectroscopic observations of HerMES and H-ATLAS-selected lensed galaxies

    Science.gov (United States)

    Cooray, Asantha

    In the quest to develop a fundamental understanding of galaxy formation and evolution, observations of dusty star-forming galaxies (DSFGs) promise significant progress this decade. The importance of DSFGs is highlighted by the fact that half of the energy emitted by extragalactic sources emerges as dust-reprocessed light at infrared (IR) to sub millimeter wavelength. In the post-herschel\\ era, we are now at a unique position to tackle some of the key questions on galaxy formation and evolution because of the large area Herschel's Key Project surveys (HerMES and H-ATLAS). In particular those surveys have allowed us to identify a sample of 250 strongly gravitationally lensed DSFGs at z > 1. They give us a unique opportunity to dissect the detailed structures and kinematics of DSFGs. The Herschel Science Archive also contains individual follow up data on 44 and 25 of the brightest sources with SPIRE-FTS and PACS, respectively, in the spectroscopy mode, taking over 250 hours in four open-time programs. Only one of the 44 SPIRE FTS targets has yet to appear in the published literature. One of the four include an open-time 2 PACS spectroscopy program that was led at UCI by a former postdoc from the PI's group. That program was initially approved at Priority 2 in 2011, but was triggered in late 2012 and achieved 100% completion during the last two weeks of Herschel lifetime in May 2013. This archival analysis, interpretation, and modeling program involves two parts: (i) PACS spectroscopy in 50 to 200 microns of 25 lensed galaxies in the fine-structure emission lines [SiII]34, [SIII]33, [OIV]26, [OIII]52, [NIII]57 and [OI]63, and the molecular hydrogen H_2 S(0) and S(1). (ii) SPIRE FTS spectroscopy of 44 lensed galaxies, including above 25, over the wavelength range of 200 to 600 microns targeting [CII]158, [OIII]88, [OI]63/145, and [NI]122. The analysis will lead to a better understanding of the ISM of starbursting galaxies that span 1 research supports Goal 2 of the

  8. Involving the public into HEP through IT challenges and projects

    CERN Document Server

    Adam Bourdarios, Claire; The ATLAS collaboration

    2016-01-01

    The ATLAS collaboration has recently setup three outreach projects and global challenges which have a strong IT component and could not have been envisaged without the growth of general public computing resources and network connectivity. HEP has exciting and difficult problems like the extraction of the Higgs boson signal, and at the same time data scientists have advanced algorithms. The goal of the Higgs Machine Learning (HiggsML) project was to bring the two together by a “challenge”: machine learning experts could compete online to obtain the best Higgs→ττ signal significance on a set of ATLAS fully simulated Monte Carlo signal and background events. The first challenge of this kind ran from May to September 2014, drawing considerable attention, and new projects followed in the context of the CERN open data initiative. Higgs Hunters is the only physics-related project hosted on a web-based citizen science platform called Zooniverse. Volunteers usually contributing to space, natural world and huma...

  9. An electronic logbook for the HEP control room

    International Nuclear Information System (INIS)

    Roediger, G.; Pomatto, P.; Kyriakopulos, J.; Panacek, S.; Canal, P.; Kubarovsky, A.

    2001-01-01

    The Control Room Logbook (CRL) is designed to improve and replace the paper logbooks traditionally used in the HEP accelerator control room. Its features benefit the on-line coordinator, the shift operators, and the remote observers. The author explains some of the most attractive features for each of these roles. The features include the ability to configure the logbook for the specific needs of a collaboration, a large variety of entry types an operator can add by simply clicking and dragging, and a flexible web interface for the remote observer to keep up with control room activities. The entries and saved as UTF-8 based XML files, which allowed us to give the data structure and meaning such that it can easily be parsed in the present and far into the future. The XML tag data is also indexed in a relational database, making queries on dates, keywords, entry type and other criteria feasible and fast. The CRL is used in the D0 control room. This presentation also discusses our experience with deployment, platform independence and other interesting issues that arose with the installation and use of logbook

  10. An Electronic Logbook for the HEP Control Room

    International Nuclear Information System (INIS)

    Gary Roediger

    2001-01-01

    The Control Room Logbook (CRL) is designed to improve and replace the paper logbooks traditionally used in the HEP accelerator control room. Its features benefit the on-line coordinator, the shift operators, and the remote observers. This paper explains some of the most attractive features for each of these roles. The features include the ability to configure the logbook for the specific needs of a collaboration, a large variety of entry types an operator can add by simply clicking and dragging, and a flexible web interface for the remote observer to keep up with control room activities. The entries are saved as UTF-8 based XML files, which allowed us to give the data structure and meaning such that it can easily be parsed in the present and far into the future. The XML tag data is also indexed in a relational database, making queries on dates, keywords, entry type and other criteria feasible and fast. The CRL is used in the D0 control room. This presentation also discusses our experience with deployment, platform independence and other interesting issues that arose with the installation and use of the logbook

  11. Wide area network monitoring system for HEP experiments at Fermilab

    International Nuclear Information System (INIS)

    Grigoriev, Maxim; Fermilab; Cottrell, Les; Logg, Connie; SLAC

    2004-01-01

    Large, distributed High Energy Physics (HEP) collaborations, such as D0, CDF and US-CMS, depend on stable and robust network paths between major world research centres. The evolving emphasis on data and compute Grids increases the reliance on network performance. Fermilab's experimental groups and network support personnel identified a critical need for WAN monitoring to ensure the quality and efficient utilization of such network paths. This has led to the development of the Network Monitoring system we will present in this paper. The system evolved from the IEPM-BW project, started at SLAC three years ago. At Fermilab this system has developed into a fully functional infrastructure with bi-directional active network probes and path characterizations. It is based on the Iperf achievable throughput tool, Ping and Synack to test ICMP/TCP connectivity. It uses Pipechar and Traceroute to test, compare and report hop-by-hop network path characterization. It also measures real file transfer performance by BBFTP and GridFTP. The Monitoring system has an extensive web-interface and all the data is available through standalone SOAP web services or by a MonaLISA client. Also in this paper we will present a case study of network path asymmetry and abnormal performance between FNAL and SDSC, which was discovered and resolved by utilizing the Network Monitoring system

  12. Wide Area Network Monitoring System for HEP Experiments at Fermilab

    International Nuclear Information System (INIS)

    Grigoriev, M.

    2004-01-01

    Large, distributed High Energy Physics (HEP) collaborations, such as D0, CDF and US-CMS, depend on stable and robust network paths between major world research centres. The evolving emphasis on data and compute Grids increases the reliance on network performance. Fermilab's experimental groups and network support personnel identified a critical need for WAN monitoring to ensure the quality and efficient utilization of such network paths. This has led to the development of the Network Monitoring system we will present in this paper. The system evolved from the IEPM-BW project, started at SLAC three years ago. At Fermilab this system has developed into a fully functional infrastructure with bi-directional active network probes and path characterizations. It is based on the Iperf achievable throughput tool, Ping and Synack to test ICMP/TCP connectivity. It uses Pipechar and Traceroute to test, compare and report hop-by-hop network path characterization. It also measures real file transfer performance by BBFTP and GridFTP. The Monitoring system has an extensive web-interface and all the data is available through standalone SOAP web services or by a MonaLISA client. Also in this paper we will present a case study of network path asymmetry and abnormal performance between FNAL and SDSC, which was discovered and resolved by utilizing the Network Monitoring system

  13. Selectivity of biopolymer membranes using HepG2 cells.

    Science.gov (United States)

    Lü, Dongyuan; Gao, Yuxin; Luo, Chunhua; Lü, Shouqian; Wang, Qian; Xu, Xianghong; Sun, Shujin; Wang, Chengzhi; Long, Mian

    2015-03-01

    Bioartificial liver (BAL) system has emerged as an alternative treatment to bridge acute liver failure to either liver transplantation or liver regeneration. One of the main reasons that the efficacy of the current BAL systems was not convincing in clinical trials is attributed to the lack of friendly interface between the membrane and the hepatocytes in liver bioreactor, the core unit of BAL system. Here, we systematically compared the biological responses of hepatosarcoma HepG2 cells seeded on eight, commercially available biocompatible membranes made of acetyl cellulose-nitrocellulose mixed cellulose (CA-NC), acetyl cellulose (CA), nylon (JN), polypropylene (PP), nitrocellulose (NC), polyvinylidene fluoride (PVDF), polycarbonate (PC) and polytetrafluoroethylene (PTFE). Physicochemical analysis and mechanical tests indicated that CA, JN and PP membranes yield high adhesivity and reasonable compressive and/or tensile features with friendly surface topography for cell seeding. Cells prefer to adhere on CA, JN, PP or PTFE membranes with high proliferation rate in spheriod-like shape. Actin, albumin and cytokeratin 18 expressions are favorable for cells on CA or PP membrane, whereas protein filtration is consistent among all the eight membranes. These results further the understandings of cell growth, morphology and spreading, as well as protein filtration on distinct membranes in designing a liver bioreactor.

  14. silver nanoparticles on liver cancer cells (HepG2

    Directory of Open Access Journals (Sweden)

    Ahmed I. El-Batal

    2018-01-01

    Full Text Available This study demonstrates a novel approach for the synthesis of silver nanoparticles (AgNPs against human liver cancer cell line (HepG2 using prodigiosin pigment isolated from Serratia marcescens. It further investigates the influence of various parameters such as initial pH, temperature, silver nitrate (AgNO 3 concentration, and prodigiosin concentration on stability and optical properties of synthesized prodigiosin AgNPs. Highly stable, spherical prodigiosin-conjugated AgNPs were synthesized with a mean diameter of 9.98 nm using a rapid one-step method. The cytotoxic activity investigated in the present study indicated that prodigiosin and prodigiosin-conjugated AgNPs possessed a strong cytotoxic potency against human liver cancer. The In silico molecular docking results of prodigiosin and prodigiosin-conjugated AgNPs are congruent with the In vitro studies and these AgNPs can be considered as good inhibitors of mitogen-activated protein kinase 1 (MEK kinases. The study opened the possibility of using prodigiosin-conjugated AgNPs to increase the efficiency of liver cancer treatment.

  15. Wide Area Network Monitoring System for HEP Experiments at Fermilab

    Energy Technology Data Exchange (ETDEWEB)

    Grigoriev, M.

    2004-11-23

    Large, distributed High Energy Physics (HEP) collaborations, such as D0, CDF and US-CMS, depend on stable and robust network paths between major world research centres. The evolving emphasis on data and compute Grids increases the reliance on network performance. Fermilab's experimental groups and network support personnel identified a critical need for WAN monitoring to ensure the quality and efficient utilization of such network paths. This has led to the development of the Network Monitoring system we will present in this paper. The system evolved from the IEPM-BW project, started at SLAC three years ago. At Fermilab this system has developed into a fully functional infrastructure with bi-directional active network probes and path characterizations. It is based on the Iperf achievable throughput tool, Ping and Synack to test ICMP/TCP connectivity. It uses Pipechar and Traceroute to test, compare and report hop-by-hop network path characterization. It also measures real file transfer performance by BBFTP and GridFTP. The Monitoring system has an extensive web-interface and all the data is available through standalone SOAP web services or by a MonaLISA client. Also in this paper we will present a case study of network path asymmetry and abnormal performance between FNAL and SDSC, which was discovered and resolved by utilizing the Network Monitoring system.

  16. A Security Monitoring Framework For Virtualization Based HEP Infrastructures

    Science.gov (United States)

    Gomez Ramirez, A.; Martinez Pedreira, M.; Grigoras, C.; Betev, L.; Lara, C.; Kebschull, U.; ALICE Collaboration

    2017-10-01

    High Energy Physics (HEP) distributed computing infrastructures require automatic tools to monitor, analyze and react to potential security incidents. These tools should collect and inspect data such as resource consumption, logs and sequence of system calls for detecting anomalies that indicate the presence of a malicious agent. They should also be able to perform automated reactions to attacks without administrator intervention. We describe a novel framework that accomplishes these requirements, with a proof of concept implementation for the ALICE experiment at CERN. We show how we achieve a fully virtualized environment that improves the security by isolating services and Jobs without a significant performance impact. We also describe a collected dataset for Machine Learning based Intrusion Prevention and Detection Systems on Grid computing. This dataset is composed of resource consumption measurements (such as CPU, RAM and network traffic), logfiles from operating system services, and system call data collected from production Jobs running in an ALICE Grid test site and a big set of malware samples. This malware set was collected from security research sites. Based on this dataset, we will proceed to develop Machine Learning algorithms able to detect malicious Jobs.

  17. Xanthorrhizol induced DNA fragmentation in HepG2 cells involving Bcl-2 family proteins

    International Nuclear Information System (INIS)

    Tee, Thiam-Tsui; Cheah, Yew-Hoong; Meenakshii, Nallappan; Mohd Sharom, Mohd Yusof; Azimahtol Hawariah, Lope Pihie

    2012-01-01

    Highlights: ► We isolated xanthorrhizol, a sesquiterpenoid compound from Curcuma xanthorrhiza. ► Xanthorrhizol induced apoptosis in HepG2 cells as observed using SEM. ► Apoptosis in xanthorrhizol-treated HepG2 cells involved Bcl-2 family proteins. ► DNA fragmentation was observed in xanthorrhizol-treated HepG2 cells. ► DNA fragmentation maybe due to cleavage of PARP and DFF45/ICAD proteins. -- Abstract: Xanthorrhizol is a plant-derived pharmacologically active sesquiterpenoid compound isolated from Curcuma xanthorrhiza. Previously, we have reported that xanthorrhizol inhibited the proliferation of HepG2 human hepatoma cells by inducing apoptotic cell death via caspase activation. Here, we attempt to further elucidate the mode of action of xanthorrhizol. Apoptosis in xanthorrhizol-treated HepG2 cells as observed by scanning electron microscopy was accompanied by truncation of BID; reduction of both anti-apoptotic Bcl-2 and Bcl-X L expression; cleavage of PARP and DFF45/ICAD proteins and DNA fragmentation. Taken together, these results suggest xanthorrhizol as a potent antiproliferative agent on HepG2 cells by inducing apoptosis via Bcl-2 family members. Hence we proposed that xanthorrhizol could be used as an anti-liver cancer drug for future studies.

  18. Xanthorrhizol induced DNA fragmentation in HepG2 cells involving Bcl-2 family proteins

    Energy Technology Data Exchange (ETDEWEB)

    Tee, Thiam-Tsui, E-mail: thiamtsu@yahoo.com [School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia); Cheah, Yew-Hoong [School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia); Bioassay Unit, Herbal Medicine Research Center, Institute for Medical Research, Jalan Pahang, Kuala Lumpur (Malaysia); Meenakshii, Nallappan [Biology Department, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Mohd Sharom, Mohd Yusof; Azimahtol Hawariah, Lope Pihie [School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor (Malaysia)

    2012-04-20

    Highlights: Black-Right-Pointing-Pointer We isolated xanthorrhizol, a sesquiterpenoid compound from Curcuma xanthorrhiza. Black-Right-Pointing-Pointer Xanthorrhizol induced apoptosis in HepG2 cells as observed using SEM. Black-Right-Pointing-Pointer Apoptosis in xanthorrhizol-treated HepG2 cells involved Bcl-2 family proteins. Black-Right-Pointing-Pointer DNA fragmentation was observed in xanthorrhizol-treated HepG2 cells. Black-Right-Pointing-Pointer DNA fragmentation maybe due to cleavage of PARP and DFF45/ICAD proteins. -- Abstract: Xanthorrhizol is a plant-derived pharmacologically active sesquiterpenoid compound isolated from Curcuma xanthorrhiza. Previously, we have reported that xanthorrhizol inhibited the proliferation of HepG2 human hepatoma cells by inducing apoptotic cell death via caspase activation. Here, we attempt to further elucidate the mode of action of xanthorrhizol. Apoptosis in xanthorrhizol-treated HepG2 cells as observed by scanning electron microscopy was accompanied by truncation of BID; reduction of both anti-apoptotic Bcl-2 and Bcl-X{sub L} expression; cleavage of PARP and DFF45/ICAD proteins and DNA fragmentation. Taken together, these results suggest xanthorrhizol as a potent antiproliferative agent on HepG2 cells by inducing apoptosis via Bcl-2 family members. Hence we proposed that xanthorrhizol could be used as an anti-liver cancer drug for future studies.

  19. Evaluation of Novel 64Cu-Labeled Theranostic Gadolinium-Based Nanoprobes in HepG2 Tumor-Bearing Nude Mice

    Science.gov (United States)

    Hu, Pengcheng; Cheng, Dengfeng; Huang, Tao; Banizs, Anna B.; Xiao, Jie; Liu, Guobing; Chen, Quan; Wang, Yuenan; He, Jiang; Shi, Hongcheng

    2017-09-01

    Radiation therapy of liver cancer is limited by low tolerance of the liver to radiation. Radiosensitizers can effectively reduce the required radiation dose. AGuIX nanoparticles are small, multifunctional gadolinium-based nanoparticles that can carry radioisotopes or fluorescent markers for single-photon emission computed tomography (SPECT), positron emission tomography (PET), fluorescence imaging, and even multimodality imaging. In addition, due to the high atomic number of gadolinium, it can also serve as a tumor radiation sensitizer. It is critical to define the biodistribution and pharmacokinetics of these gadolinium-based nanoparticles to quantitate the magnitude and duration of their retention within the tumor microenvironment during radiotherapy. Therefore, in this study, we successfully labeled AGuIX with 64Cu through the convenient built-in chelator. The biodistribution studies indicated that the radiotracer 64Cu-AGuIX accumulates to high levels in the HepG2 xenograft of nude mice, suggesting that it would be a potential theranostic nanoprobe for image-guided radiotherapy in HCC. We also used a transmission electron microscope to confirm AGuIX uptake in the HepG2 cells. In radiation therapy studies, a decrease in 18F-FDG uptake was observed in the xenografts of the nude mice irradiated with AGuIX, which was injected 1 h before. These results provide proof-of-concept that AGuIX can be used as a theranostic radiosensitizer for PET imaging to guide radiotherapy for liver cancer.

  20. iTRAQ protein profile analysis of Neuroblastoma (NA cells infected with the Rabies Viruses rHep-Flury and Hep-dG

    Directory of Open Access Journals (Sweden)

    Youtian eYang

    2015-07-01

    Full Text Available The rabies virus (RABV glycoprotein (G is the principal contributor to the pathogenicity and protective immunity of RABV. In a previous work, we reported that recombinant rabies virus Hep-dG, which was generated by reverse genetics to carry two copies of the G-gene, showed lower virulence than the parental virus rHep-Flury in suckling mice with a better immune protection effect. To better understand the mechanisms underlying rabies virus attenuation and the role of glycoprotein G, isobaric tags for relative and absolute quantitation (iTRAQ was performed to identify and quantify distinct proteins. 10 and 111 differentially expressed proteins were obtained in rHep-Flury and Hep-dG infection groups, respectively. Selected data were validated by western blot and qRT-PCR. Bioinformatics analysis of the distinct protein suggested that glycoprotein over-expression in the attenuated RABV strain can induce activation of the interferon signaling. Furthermore, it may promote the antiviral response, MHC-I mediated antigen-specific T cell immune response, apoptosis and autophagy in an IFN-dependent manner. These findings might not only improve the understanding of the dynamics of RABV and host interaction, but also help understand the mechanisms underlying innate and adaptive immunity during RABV infection.

  1. Radiation induced bystander effect on hepatoma HepG2 cells under hypoxia condition

    International Nuclear Information System (INIS)

    Zhang Jianghong; Jin Yizun; Shao Chunlin; Prise KM

    2009-01-01

    Objective: To investigate radiation induced bystander effect and its mechanism on hepatoma HepG2 cells under hypoxia condition. Methods: Non-irradiated bystander hepatoma cells were co-cultured with irradiated cells or treated with the conditioned medium (CM) from irradiated cells, then micronuclei (MN) were measured for both irradiated cells and bystander cells. Results: The MN yield of irradiated HepG2 cells under hypoxic condition was significantly lower than that under normoxia, the oxygen enhancement ratio of HepG2 cells of MN was 1.6. For both hypoxic and normoxic condition, the MN yield of bystander cells were obviously enhanced to a similar high level after co-culturing with irradiated cells or with CM treatment, and it also correlated with the irradiation dose. When the hypoxic HepG2 cells were treated with either DMSO, a scavenger of reactive oxygen species (ROS), or aminoguanidine, an iNOS inhibitor, the yield of bystander MN was partly diminished, and the reducing rate of DMSO was 42.2%-46.7%, the reducing rate of aminoguanidine was 42% . Conclusion: ROS, NO and their downstream signal factors are involved in the radiation induced bystander effect of hypoxic HepG2 cells. (authors)

  2. Habitat Evaluation Procedures (HEP) Report; Calispell Creek Project, Technical Report 2004-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On July 13, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Calispell Creek property, an acquisition completed by the Kalispel Tribe of Indians in February 2004. Evaluation species and appropriate models include Canada goose, mallard, muskrat, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Calispell Creek Project provides a total of 138.17 Habitat Units (HUs) for the species evaluated. Emergent wetland habitat provides 5.16 HUs for mallard and muskrat. Grassland provides 132.02 HUs for mallard and Canada goose. Scrub-shrub vegetation provides 0.99 HUs for yellow warbler and white-tailed deer. The objective of using HEP at the Calispell Creek Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  3. Habitat Evaluation Procedures (HEP) Report : West Beaver Lake, 2004-2005 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On September 7, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the West Beaver Lake property, an acquisition completed by the Kalispel Tribe of Indians in September 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, mallard, muskrat, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The West Beaver Lake Project provides a total of 103.08 Habitat Units (HUs) for the species evaluated. Emergent wetland habitat provides 7.17 HUs for mallard and muskrat. Conifer forest habitat provides 95.91 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. The objective of using HEP at the West Beaver Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  4. Aggregative adherent strains of Corynebacterium pseudodiphtheriticum enter and survive within HEp-2 epithelial cells

    Directory of Open Access Journals (Sweden)

    Monica Cristina de Souza

    2012-06-01

    Full Text Available Corynebacterium pseudodiphtheriticum is a well-known human pathogen that mainly causes respiratory disease and is associated with high mortality in compromised hosts. Little is known about the virulence factors and pathogenesis of C. pseudodiphtheriticum. In this study, cultured human epithelial (HEp-2 cells were used to analyse the adherence pattern, internalisation and intracellular survival of the ATCC 10700 type strain and two additional clinical isolates. These microorganisms exhibited an aggregative adherence-like pattern to HEp-2 cells characterised by clumps of bacteria with a "stacked-brick" appearance. The differences in the ability of these microorganisms to invade and survive within HEp-2 cells and replicate in the extracellular environment up to 24 h post infection were evaluated. The fluorescent actin staining test demonstrated that actin polymerisation is involved in the internalisation of the C. pseudodiphtheriticum strains. The depolymerisation of microfilaments by cytochalasin E significantly reduced the internalisation of C. pseudodiphtheriticum by HEp-2 cells. Bacterial internalisation and cytoskeletal rearrangement seemed to be partially triggered by the activation of tyrosine kinase activity. Although C. pseudodiphtheriticum strains did not demonstrate an ability to replicate intracellularly, HEp-2 cells were unable to fully clear the pathogen within 24 h. These characteristics may explain how some C. pseudodiphtheriticum strains cause severe infection in human patients.

  5. Images.

    Science.gov (United States)

    Barr, Catherine, Ed.

    1997-01-01

    The theme of this month's issue is "Images"--from early paintings and statuary to computer-generated design. Resources on the theme include Web sites, CD-ROMs and software, videos, books, and others. A page of reproducible activities is also provided. Features include photojournalism, inspirational Web sites, art history, pop art, and myths. (AEF)

  6. Archiving Scientific Data Outside of the Traditional HEP Domain, Using the Archive Facilities at Fermilab

    International Nuclear Information System (INIS)

    Norman, A.; Diesbug, M.; Gheith, M.; Illingworth, R.; Mengel, M.

    2015-01-01

    Many experiments in the HEP and Astrophysics communities generate large extremely valuable datasets, which need to be efficiently cataloged and recorded to archival storage. These datasets, both new and legacy, are often structured in a manner that is not conducive to storage and cataloging with modern data handling systems and large file archive facilities. In this paper we discuss in detail how we have created a robust toolset and simple portal into the Fermilab archive facilities, which allows for scientific data to be quickly imported, organized and retrieved from the multi-petabyte facility.In particular we discuss how the data from the Sudbury Neutrino Observatory (SNO) for the COUPP dark matter detector was aggregated, cataloged, archived and re-organized to permit it to be retrieved and analyzed using modern distributed computing resources both at Fermilab and on the Open Science Grid. We pay particular attention to the methods that were employed to uniquify the namespaces for the data, derive metadata for the over 460,000 image series taken by the COUP experiment and what was required to map that information into coherent datasets that could be stored and retrieved using the large scale archives systems.We describe the data transfer and cataloging engines that are used for data importation and how these engines have been setup to import data from the data acquisition systems of ongoing experiments at non-Fermilab remote sites including the Laboratori Nazionali del Gran Sasso and the Ash River Laboratory in Orr, Minnesota. We also describe how large University computing sites around the world are using the system to store and retrieve large volumes of simulation and experiment data for physics analysis. (paper)

  7. Comparative cytotoxic response of nickel ferrite nanoparticles in human liver HepG2 and breast MFC-7 cancer cells.

    Science.gov (United States)

    Ahamed, Maqusood; Akhtar, Mohd Javed; Alhadlaq, Hisham A; Khan, M A Majeed; Alrokayan, Salman A

    2015-09-01

    Nickel ferrite nanoparticles (NPs) have received much attention for their potential applications in biomedical fields such as magnetic resonance imaging, drug delivery and cancer hyperthermia. However, little is known about the toxicity of nickel ferrite NPs at the cellular and molecular levels. In this study, we investigated the cytotoxic responses of nickel ferrite NPs in two different types of human cells (i.e., liver HepG2 and breast MCF-7). Nickel ferrite NPs induced dose-dependent cytotoxicity in both types of cells, which was demonstrated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT), neutral red uptake (NRU) and lactate dehydrogenase (LDH) assays. Nickel ferrite NPs were also found to induce oxidative stress, which was evident by the depletion of glutathione and the induction of reactive oxygen species (ROS) and lipid peroxidation. The mitochondrial membrane potential due to nickel ferrite NP exposure was also observed. The mRNA levels for the tumor suppressor gene p53 and the apoptotic genes bax, CASP3 and CASP9 were up-regulated, while the anti-apoptotic gene bcl-2 was down-regulated following nickel ferrite NP exposure. Furthermore, the activities of apoptotic enzymes (caspase-3 and caspase-9) were also higher in both types of cells treated with nickel ferrite NPs. Cytotoxicity induced by nickel ferrite was efficiently prevented by N-acetyl cysteine (ROS scavenger) treatment, which suggested that oxidative stress might be one of the possible mechanisms of nickel ferrite NP toxicity. We also observed that MCF-7 cells were slightly more susceptible to nickel ferrite NP exposure than HepG2 cells. This study warrants further investigation to explore the potential mechanisms of different cytotoxic responses of nickel ferrite NPs in different cell lines. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Offering Global Collaboration Services beyond CERN and HEP

    Science.gov (United States)

    Fernandes, J.; Ferreira, P.; Baron, T.

    2015-12-01

    The CERN IT department has built over the years a performant and integrated ecosystem of collaboration tools, from videoconference and webcast services to event management software. These services have been designed and evolved in very close collaboration with the various communities surrounding the laboratory and have been massively adopted by CERN users. To cope with this very heavy usage, global infrastructures have been deployed which take full advantage of CERN's international and global nature. If these services and tools are instrumental in enabling the worldwide collaboration which generates major HEP breakthroughs, they would certainly also benefit other sectors of science in which globalization has already taken place. Some of these services are driven by commercial software (Vidyo or Wowza for example), some others have been developed internally and have already been made available to the world as Open Source Software in line with CERN's spirit and mission. Indico for example is now installed in 100+ institutes worldwide. But providing the software is often not enough and institutes, collaborations and project teams do not always possess the expertise, or human or material resources that are needed to set up and maintain such services. Regional and national institutions have to answer needs, which are growingly global and often contradict their operational capabilities or organizational mandate and so are looking at existing worldwide service offers such as CERN's. We believe that the accumulated experience obtained through the operation of a large scale worldwide collaboration service combined with CERN's global network and its recently- deployed Agile Infrastructure would allow the Organization to set up and operate collaborative services, such as Indico and Vidyo, at a much larger scale and on behalf of worldwide research and education institutions and thus answer these pressing demands while optimizing resources at a global level. Such services would

  9. Imaging

    International Nuclear Information System (INIS)

    Kellum, C.D.; Fisher, L.M.; Tegtmeyer, C.J.

    1987-01-01

    This paper examines the advantages of the use of excretory urography for diagnosis. According to the authors, excretory urography remains the basic radiologic examination of the urinary tract and is the foundation for the evaluation of suspected urologic disease. Despite development of the newer diagnostic modalities such as isotope scanning, ultrasonography, CT, and magnetic resonsance imaging (MRI), excretory urography has maintained a prominent role in ruorradiology. Some indications have been altered and will continue to change with the newer imaging modalities, but the initial evaluation of suspected urinary tract structural abnormalities; hematuria, pyuria, and calculus disease is best performed with excretory urography. The examination is relatively inexpensive and simple to perform, with few contraindictions. Excretory urography, when properly performed, can provide valuable information about the renal parenchyma, pelvicalyceal system, ureters, and urinary bladder

  10. Tumores hepáticos incomuns: ensaio iconográfico – Parte 2

    Directory of Open Access Journals (Sweden)

    Bruno Cheregati Pedrassa

    2014-12-01

    Full Text Available O diagnóstico das lesões hepáticas mais frequentes pode ser feito com alguma segurança quando exibem aspectos típicos, utilizandose os diversos métodos de imagem; ao contrário, as lesões incomuns são geralmente um desafio diagnóstico para o radiologista. Nesta segunda parte do estudo serão descritas quatro raras lesões hepáticas: o linfoma hepático primário, o tumor miofibroblástico, o carcinoma neuroendócrino primário do fígado e o tumor desmoplásico de pequenas células, abordando as suas principais características e achados de imagem, com foco na tomografia computadorizada e ressonância magnética.

  11. Esteatose hepática em crianças e adolescentes obesos

    OpenAIRE

    Duarte, Maria Amélia S. M.; Silva, Giselia Alves Pontes da

    2011-01-01

    OBJETIVO: Descrever a frequência de esteatose hepática e da síndrome metabólica em um grupo de crianças e adolescentes obesos. MÉTODO: Estudo descritivo, do tipo série de casos, com 77 pacientes analisados, entre 2 anos e 13 anos e 11 meses de idade, no período de fevereiro a julho de 2007. Obesidade foi definida como índice de massa corporal > P95 para idade e sexo. O diagnóstico de esteatose hepática foi feito por ultrassonografia hepática. A síndrome metabólica foi definida segundo os crit...

  12. Estudio del estado bucodental del paciente trasplantado hepático

    OpenAIRE

    Díaz Ortiz, María Luisa; Micó Llorens, José María; Gargallo Albiol, Jordi; Baliellas Comellas, Carmen; Berini Aytés, Leonardo; Gay Escoda, Cosme

    2005-01-01

    Desde que se realizó en España el primer trasplante hepático en el año 1984 los avances en la técnica quirúrgica y en los fármacos inmunosupresores empleados han producido un aumento en el número de pacientes trasplantados. El objetivo del presente estudio fue valorar el estado bucodental de los pacientes trasplantados hepáticos. Se realizó un estudio descriptivo transversal de una muestra de pacientes que habían sido sometidos a un trasplante hepático en el Hospital Príncipes de España de la...

  13. The HepMC C++ Monte Carlo Event Record for High Energy Physics

    CERN Document Server

    Dobbs, M

    2000-01-01

    HepMC is an Object Oriented event record written in C++ for High Energy Physics Monte Carlo Event Generators. Many extensions from HEPEVT, the Fortran HEP standard, are supported: the number of entries is unlimited, spin density matrices can be stored with each vertex, flow patterns (such as colour) can be stored and traced, random number generator states can be stored, and an arbitrary number of event weights can be included. Particles and vertices are stored separately in a graph structure, reflecting the evolution of a physics event. The added information supports the modularisation of event generators. The event record has been kept as simple as possible with minimal internal/external dependencies. Event information is accessed by means of iterators supplied with HepMC.

  14. Langston University - High Energy Physics (LU-HEP)

    Energy Technology Data Exchange (ETDEWEB)

    Snow, Dr., Joel [Langston Univ., OK (United States)

    2012-08-13

    PI served as MC production coordinator MC production increased very significantly. In the first year of the PI's tenure as production coordinator production was 159M events and 6.7~TB of data. During the last year of the project period production was 2,342~M events and 262~TB of data. That is a factor of 15 increase in events and 39 in data volume. The increase occurred with improvements in computer hardware and networks, through the use of grid technology on diverse resources, and through increased automation and efficiency of the production process. LU HEP developed and deployed the automatic MC request processing system in use at FNAL. The complementary strategies of automation and grid production served DZero well. Fermilab has recognized LU HEP's contribution to DZero by allowing the PI to devote full time to research activities by appointing him a guest scientist for the last six years of the project period.

  15. Habitat Evaluation Procedures (HEP) Report Wanaket Wildlife Area, Techical Report 2005-2006.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul

    2006-02-01

    The Regional HEP Team (RHT) and Confederated Tribes of the Umatilla Indian Reservation (CTUIR) Wildlife Program staff conducted a follow-up habitat evaluation procedures (HEP) analysis on the Wanaket Wildlife Management Area in June 2005. The 2005 HEP investigation generated 3,084.48 habitat units (HUs) for a net increase of 752.18 HUs above 1990/1995 baseline survey results. The HU to acre ratio also increased from 0.84:1.0 to 1.16:1.0. The largest increase in habitat units occurred in the shrubsteppe/grassland cover type (California quail and western meadowlark models), which increased from 1,544 HUs to 2,777 HUs (+43%), while agriculture cover type HUs were eliminated because agricultural lands (managed pasture) were converted to shrubsteppe/grassland. In addition to the agriculture cover type, major changes in habitat structure occurred in the shrubsteppe/grassland cover type due to the 2001 wildfire which removed the shrub component from well over 95% of its former range. The number of acres of all other cover types remained relatively stable; however, habitat quality improved in the riparian herb and riparian shrub cover types. The number and type of HEP species models used during the 2005 HEP analysis were identical to those used in the 1990/1995 baseline HEP surveys. The number of species models employed to evaluate the shrubsteppe/grassland, sand/gravel/mud/cobble, and riparian herb cover types, however, were fewer than reported in the McNary Dam Loss Assessment (Rassmussen and Wright 1989) for the same cover types.

  16. DQM4HEP - A Generic Online Monitor for Particle Physics Experiments

    CERN Document Server

    Coates, Tom; Salvatore, Fabrizio; Cussans, David; Ete, Remi; Irles, Adrian; Mirabito, Lauren; Pingault, Antoine; Wing, Matthew

    2018-01-01

    Currently there is a lot of activity in R&D for future colliders. Multiple detector prototypes are being tested, each with different requirements for data acquisition and monitoring, which has generated different ad-hoc software solutions. We present DQM4HEP, a generic C++11 framework for online monitoring for particle physics experiments, and results obtained at several testbeams with detector prototypes using the framework as it was developed. We also present the currently ongoing work to integrate DQM4HEP and EUDAQ, which will allow these to work together as a complete and generic DAQ and monitoring system for any detector test, as part of AIDA-2020.

  17. ATLAS Experience with HEP Software at the Argonne Leadership Computing Facility

    CERN Document Server

    LeCompte, T; The ATLAS collaboration; Benjamin, D

    2014-01-01

    A number of HEP software packages used by the ATLAS experiment, including GEANT4, ROOT and ALPGEN, have been adapted to run on the IBM Blue Gene supercomputers at the Argonne Leadership Computing Facility. These computers use a non-x86 architecture and have a considerably less rich operating environment than in common use in HEP, but also represent a computing capacity an order of magnitude beyond what ATLAS is presently using via the LCG. The status and potential for making use of leadership-class computing, including the status of integration with the ATLAS production system, is discussed.

  18. Revisión de casos de tumores hepáticos

    OpenAIRE

    Salazar Sánchez, Augusto

    2011-01-01

    Se revisaron las historias clínicas de 126 pacientes ingresados al Hospital de San Juan de Dios con el diagnóstico de tumor hepático, durante los años de 1952 a 1956. De ellas se desecharon aquellos casos que correspondieron a tumores metastásicos, dejando tan solo 18, que histológicamente correspondieron a tumores primitivamente hepáticos; además se incluyen en este trabajo dos historias de pacientes atendidos en el Instituto Colombiano de Seguros Sociales y tres de clientela particular....

  19. Revisión de casos de tumores hepáticos

    Directory of Open Access Journals (Sweden)

    Augusto Salazar Sánchez

    1956-09-01

    Full Text Available Se revisaron las historias clínicas de 126 pacientes ingresados al Hospital de San Juan de Dios con el diagnóstico de tumor hepático, durante los años de 1952 a 1956. De ellas se desecharon aquellos casos que correspondieron a tumores metastásicos, dejando tan solo 18, que histológicamente correspondieron a tumores primitivamente hepáticos; además se incluyen en este trabajo dos historias de pacientes atendidos en el Instituto Colombiano de Seguros Sociales y tres de clientela particular.

  20. HepSim: A repository with predictions for high-energy physics experiments

    International Nuclear Information System (INIS)

    Chekanov, S. V.

    2015-01-01

    A file repository for calculations of cross sections and kinematic distributions using Monte Carlo generators for high-energy collisions is discussed. The repository is used to facilitate effective preservation and archiving of data from theoretical calculations and for comparisons with experimental data. The HepSim data library is publicly accessible and includes a number of Monte Carlo event samples with Standard Model predictions for current and future experiments. The HepSim project includes a software package to automate the process of downloading and viewing online Monte Carlo event samples. Data streaming over a network for end-user analysis is discussed

  1. ATLAS experience with HEP software at the Argonne leadership computing facility

    International Nuclear Information System (INIS)

    Uram, Thomas D; LeCompte, Thomas J; Benjamin, D

    2014-01-01

    A number of HEP software packages used by the ATLAS experiment, including GEANT4, ROOT and ALPGEN, have been adapted to run on the IBM Blue Gene supercomputers at the Argonne Leadership Computing Facility. These computers use a non-x86 architecture and have a considerably less rich operating environment than in common use in HEP, but also represent a computing capacity an order of magnitude beyond what ATLAS is presently using via the LCG. The status and potential for making use of leadership-class computing, including the status of integration with the ATLAS production system, is discussed.

  2. Fiabilidade do modelo Lok no diagnóstico da cirrose hepática

    OpenAIRE

    Costa, Jennifer Lima da

    2010-01-01

    Trabalho final de mestrado integrado em Medicina área cientifica de Gastroenterologia, apresentado à Faculdade de Medicina da Universidade de Coimbra Introdução: Marcadores serológicos que permitem avaliar a presença de cirrose hepática têm sido intensamente estudados. Contudo, a biópsia hepática continua a ser o Gold-standard para avaliar o grau de fibrose. O modelo de Lok é um teste indirecto que consiste numa fórmula que analisa marcadores indirectos de fibrose e foi formulado para perm...

  3. Benefits of diverse and interdisciplinary co-creation for HEP - a showcase

    CERN Document Server

    Castro, Cristina Bahamonde; Dopke, Jens; Kagan, Michael; Kaufmann, Sylvain; Knaepper, Ines; Kurikka, Joona; Milano, Leonardo; Muranaka, Tomoko; Pascu, Iulia; Potamianos, Karolos; Schweiger, Hansdieter; Utriainen, Tuuli; Perez Codina, Estel; Dobos, Daniel

    2016-01-01

    THE Port association organises interdisciplinary co-creational humanitarian hackathons at CERN. Combining physicists and engineers working on HEP related topics in their day job with entrepreneurs, artists, researchers, designers, humanitarian workers and other creative minds helps identifying similar material and engineering solutions for humanitarian challenges. It allow cross collaboration between many different disciplines. Concentrating on humanitarian and social benefitting topics the technology opportunities identify new methods, materials and processes, that can be feed back into HEP. The methodology of humanitarian hackathons is described and some examples of challenge outcomes are showcased.

  4. Síndrome hepatopulmonar como causa de hipoxemia en niños con enfermedad hepática

    Directory of Open Access Journals (Sweden)

    Jessica Sáenz-Gómez

    2015-03-01

    Conclusiones: En niños con enfermedad hepática que presenten disnea e hipoxemia, y en los que estén en protocolo de trasplante hepático, debe de buscarse intencionadamente el síndrome hepatopulmonar, ya que el pronóstico dependerá del diagnóstico oportuno.

  5. Differential genomic effects of six different TiO2 nanomaterials on human liver HepG2 cells

    Science.gov (United States)

    Engineered nanoparticles are reported to cause liver toxicity in vivo. To better assess the mechanism of the in vivo liver toxicity, we used the human hepatocarcinoma cells (HepG2) as a model system. Human HepG2 cells were exposed to 6 TiO2 nanomaterials (with dry primary partic...

  6. Linfoma não-Hodgkin apresentando-se como massa hepática única Non-Hodgkin's lymphoma presenting as a single liver mass

    Directory of Open Access Journals (Sweden)

    Mila Correia Góis Peixoto

    2009-02-01

    Full Text Available OBJETIVO: Descrever as principais características de imagem do linfoma não-Hodgkin apresentando-se como massa hepática única. MATERIAIS E MÉTODOS: Realizamos estudo retrospectivo mediante análise de casos de pacientes com massa hepática única aos exames de ultrassonografia, tomografia computadorizada e ressonância magnética, com diagnóstico histológico de linfoma não-Hodgkin. Esses exames foram analisados por dois examinadores em consenso. RESULTADOS: Identificamos três pacientes, todos do sexo masculino, na quinta década de vida, com quadro clínico inespecífico e que apresentavam massa hepática única e com diagnóstico de linfoma não-Hodgkin. Na ultrassonografia a lesão hepática apresentava-se como massa com aspecto "em alvo" nos três casos estudados. Na tomografia computadorizada observou-se massa hipodensa e heterogênea, com realce anelar em todos os casos. Na ressonância magnética as lesões apresentavam-se heterogêneas, hipointensas em T1 e hiperintensas em T2, e também com realce anelar após a injeção do contraste. Nenhum paciente apresentava linfonodomegalia ou comprometimento de outras vísceras sólidas no momento do diagnóstico. CONCLUSÃO: Na presença de massa hepática solitária e com aspecto "em alvo" deve-se considerar, entre as hipóteses, o diagnóstico de linfoma.OBJECTIVE: To describe the main imaging findings of non-Hodgkin's lymphoma presenting as a single liver mass. MATERIALS AND METHODS: A retrospective study was developed with analysis of cases where a single liver mass was observed at ultrasonography, computed tomography and magnetic resonance imaging, and histologically diagnosed as non-Hodgkin's lymphoma. The studies were reviewed by two observers in consensus. RESULTS: Three male patients in the fifth decade of life, with non-specific clinical manifestations and single liver mass diagnosed as non-Hodgkin's lymphoma were identified. A hepatic lesion with target sign was observed at

  7. Tumores hepáticos incomuns: ensaio iconográfico - Parte 1

    Directory of Open Access Journals (Sweden)

    Bruno Cheregati Pedrassa

    2014-10-01

    Full Text Available A maioria dos tumores hepáticos primários malignos é representada pelo carcinoma hepatocelular e pelo colangiocarcinoma, entretanto, uma variedade de outras lesões hepáticas incomuns pode ser encontrada. Lesões comuns como o hemangioma, a hiperplasia nodular focal e as metástases são bem conhecidas e já foram extensamente documentadas na literatura. O diagnóstico das lesões hepáticas típicas pode ser feito com alguma segurança utilizando-se os diversos métodos de imagem; por outro lado, as lesões incomuns são geralmente um desafio diagnóstico para o radiologista. Nesta primeira parte do estudo abordaremos cinco tumores hepáticos incomuns - o angiossarcoma, o angiomiolipoma, o cistoadenoma/carcinoma biliar, o hemangioendotelioma epitelioide e o carcinoma hepatocelular fibrolamelar -, suas principais características e achados de imagem, com foco na tomografia computadorizada e na ressonância magnética.

  8. DQM4HEP - A Generic Online Monitor for Particle Physics Experiments

    CERN Document Server

    Irles, Adrián; Coates, Tom; Salvatore, Fabrizio; Cussans, David; Éte, Remi; Mirabito, Laurent; Pingault, Antoine; Wing, Matthew

    2017-01-01

    There is currently a lot of activity in R&D for future collider experiments. Multiple detector prototypes are being tested, each one with slightly different requirements regarding the format of the data to be analysed. This has generated a variety of ad-hoc solutions for data acquisition and online data monitoring. We present a generic C++11 online monitoring framework called DQM4HEP, which is designed for use as a generic online monitor for particle physics experiments, ranging from small tabletop experiments to large multi-detector testbeams, such as those currently ongoing/planned at the DESY2 or CERN SPS beamlines. We present results obtained using DQM4HEP at several testbeams where the CALICE AHCAL, SDHCAL and SiWECAL detector prototypes have been tested. During these testbeams, online analysis using DQM4HEP's framework has been developed and used. We also present the currently ongoing work to integrate DQM4HEP within the EUDAQ tool. EUDAQ is a tool for common and generic data acquisition within the ...

  9. Hyperglycemia and anthocyanin inhibit quercetin metabolism in HepG2 cells

    Science.gov (United States)

    A high glucose (Glu) milieu promotes generation of reactive oxygen species, which may not only cause cellular damage, but also modulate phase II enzymes that are responsible for the metabolism of flavonoids. Thus, we examined the effect of a high Glu milieu on quercetin (Q) metabolism in HepG2 cells...

  10. Biopsia hepática por aspiración con la aguja de Menghini

    Directory of Open Access Journals (Sweden)

    Alberto Albornoz Plata

    1959-09-01

    Full Text Available Se hace una descripción de la técnica para obtener la biopsia hepática por aspiración utilizando la aguja de menghini y se explica qué es la aguja de menghini. Se presentan 10 casos en que se hizo la biopsia, casos comprendidos entre 4 y 63 años; 4 M. y 6 F.; se encontró en esos casos: hígado normal, 2 casos; cirrosis portal, 1 caso; cirrosis biliar, 2 casos; Tb. hepática, 1 caso; Hepatitis, 2 casos; Sífilis hepática, 1 caso; Ictericia extrahepática, 1 caso. Consideremos la punción biopsia hepática por aspiración por medio de la aguja menghini como el método más sencillo, inofensivo y práctico; en nuestra opinión la aguja de meghini es muy superior a la de Vim-Silverman, y en el futuro el método de menghini desplazará el método de Vim-Silverman, como ha ocurrido en el Hospital Mount Sinai de New York.

  11. 75 FR 3216 - Office of Elementary and Secondary Education; High School Equivalency Program (HEP)

    Science.gov (United States)

    2010-01-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF EDUCATION Office of Elementary and Secondary Education; High School Equivalency Program (HEP) Catalog of Federal Domestic Assistance (CFDA) Number: 84.141A. ACTION: Correction; Notice inviting applications for new awards...

  12. Distributed TRIDAQ systems for large HEP experiments: Part I. System architecture

    Science.gov (United States)

    Pozniak, Krzysztof T.

    2008-01-01

    The experiments of High Energy Physics (HEP) at the big accelerators with counter propagating beams serve for research on matter structure, properties and internal reactions. Contemporary HEP experiments are commonly done by elementary particle and nuclear physicists and engineers from many branches of technical sciences. The cutting edge level of technical complication of the experiments requires new research rather than off-the-shelf solutions. Such solutions are adopted as results of separate, large and expensive research programs, organized around a major experiment. One of the technical branches contributing essentially to the HEP experiments results is photonics and electronics. Recent application of fast, multi channel, distributed and synchronous photonic and electronic TRIDAQ systems, provides the HEP experiments with the improved space and time resolution at the measurements of positions, momenta and energies of elementary particles. TRIDAQ systems improve the selection processes of physical events (TRIgger part of the system), and the registration of relevant data (Data AcQuisition of the system). Part I of the paper discusses overall TRIDAQ system architecture, while part II of the paper shows TRIDAQ system solutions for BAC and CMS detectors, comparing the performance differences caused by two decades of system exploitation and design.

  13. NiMax: a new approach to develop hadronic event generators in HEP

    International Nuclear Information System (INIS)

    Amelin, N.; Komogorov, M.

    2000-01-01

    The NiMax framework is a new approach to develop, assemble and use hadronic event generators in HEP. There are several important concepts of the NiMax architecture: the components, the data file, the application domain module, the control system and the project. Here we describe these concepts stressing their functionality

  14. Estudo do sistema portal hepático no pato doméstico (Cairina moshata

    Directory of Open Access Journals (Sweden)

    Marcos Rogério Alves Pinto

    1999-01-01

    Full Text Available Estudou-se o comportamento do sistema portal hepático em 30 patos domésticos, adultos, machos e fêmeas. O sistema apresenta-se constituído por duas veias portais hepáticas: direita e esquerda. A veia portal hepática esquerda é formada por veias gástricas esquerdas (em número de 1 a 2, veias da margem ventral do ventrículo, veia pilórica e veia proventricular caudal. A veia portal hepática direita é formada pela veia mesentérica caudal, veia mesentérica cranial, veia proventrículo-esplênica e veia gastropancreaticoduodenal. A veia mesentérica caudal recebe tributárias do mesorreto, cloaca e junção ileocecocólica. A veia mesentérica cranial recebe tributárias jejunais (em número de 12 a 21 e se anastomosa com a veia mesentérica caudal, formando a veia mesentérica comum. A veia pancreaticoduodenal recebe duas veias gástricas direitas, constituindo assim a veia gastropancreaticoduodenal. A veia proventrículo-esplênica é formada pelas veias proventriculares dorsal e direita e pelas veias esplênicas.

  15. West Foster Creek 2007 Follow-up Habitat Evaluation Procedures (HEP) Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul R.

    2008-02-01

    A follow-up habitat evaluation procedures (HEP) analysis was conducted on the West Foster Creek (Smith acquisition) wildlife mitigation site in May 2007 to determine the number of additional habitat units to credit Bonneville Power Administration (BPA) for providing funds to enhance and maintain the project site as partial mitigation for habitat losses associated with construction of Grand Coulee Dam. The West Foster Creek 2007 follow-up HEP survey generated 2,981.96 habitat units (HU) or 1.51 HUs per acre for a 34% increase (+751.34 HUs) above baseline HU credit (the 1999 baseline HEP survey generated 2,230.62 habitat units or 1.13 HUs per acre). The 2007 follow-up HEP analysis yielded 1,380.26 sharp-tailed grouse (Tympanuchus phasianellus) habitat units, 879.40 mule deer (Odocoileus hemionus) HUs, and 722.29 western meadowlark (Sturnella neglecta) habitat units. Mule deer and sharp-tailed grouse habitat units increased by 346.42 HUs and 470.62 HUs respectively over baseline (1999) survey results due largely to cessation of livestock grazing and subsequent passive restoration. In contrast, the western meadowlark generated slightly fewer habitat units in 2007 (-67.31) than in 1999, because of increased shrub cover, which lowers habitat suitability for that species.

  16. Anti-hepatocarcinoma effects of resveratrol nanoethosomes against human HepG2 cells

    Science.gov (United States)

    Meng, Xiang-Ping; Zhang, Zhen; Chen, Tong-sheng; Wang, Yi-fei; Wang, Zhi-ping

    2017-02-01

    Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Resveratrol (Res) has been widely investigated with its strong anti-tumor activity. However, its low oral bioavailability restricts its wide application. In this study, we prepared resveratrol nanoethosomes (ResN) via ethanol injection method. The in vitro anti-hepatocarcinoma effects of ResN relative to efficacy of bulk Res were evaluated on proliferation and apoptosis of human HepG2 cells. ResN were spherical vesicles and its particle diameter, zeta potential were (115.8 +/- 1.3) nm and (-12.8 +/- 1.9) mV, respectively. ResN exhibited significant inhibitory effects against human HepG2 cells by MTT assay, and the IC50 value was 49.2 μg/ml (105.4 μg/ml of Res bulk solution). By flow cytometry assay, there was an increase in G2/M phase cells treated with ResN. The results demonstrated ResN could effectively block the G2/M phase of HepG2 cells, which can also enhance the inhibitory effect of Res against HepG2 cells.

  17. Intracellular localization of Saffold virus Leader (L) protein differs in Vero and HEp-2 cells.

    Science.gov (United States)

    Xu, Yishi; Victorio, Carla Bianca Luena; Ng, Qimei; Prabakaran, Mookkan; Tan, Yee-Joo; Chua, Kaw Bing

    2016-10-12

    The Saffold virus (SAFV) genome is translated as a single long polyprotein precursor and co-translationally cleaved to yield 12 separate viral proteins. Little is known about the activities of SAFV proteins although their homologs in other picornaviruses have already been described. To further support research on functions and activities of respective viral proteins, we investigated the spatio-temporal distribution of SAFV proteins in Vero and HEp-2 cells that had been either transfected with plasmids that express individual viral proteins or infected with live SAFV. Our results revealed that, with the exception of the Leader (L) protein, all viral proteins were localized in the cytoplasm at all the time points assayed. The L protein was found in the cytoplasm at an early time point but was subsequently translocated to the nucleus of HEp-2, but not Vero, cells. This was observed in both transfected and infected cells. Further mutational analysis of L protein revealed that Threonine 58 of the Ser/Thr-rich domain of L protein is crucial for protein trafficking between the cytoplasm and nucleus in HEp-2 cells. These findings contribute to a deeper understanding and stimulate investigation of the differetial cellular responses of HEp-2 cells in comparison to other mammalian cell lines during SAFV infection.

  18. Biomarcadores para fibrosis hepática, avances, ventajas y desventajas

    Directory of Open Access Journals (Sweden)

    A. Cequera

    2014-07-01

    En este trabajo se describen los biomarcadores empleados actualmente para el estudio de la fibrosis hepática en humanos, incluyendo sus ventajas y desventajas y la implementación de tecnologías de nueva generación, y la evaluación de las posibilidades de su empleo para el diagnóstico.

  19. Enhancement of invasiveness of Yersinia enterocolitica and Escherichia coli in HEp-2 cells by centrifugation.

    OpenAIRE

    Vesikari, T; Bromirska, J; Mäki, M

    1982-01-01

    Centrifugation enhanced the infectivity of invasive Escherichia coli and Yersinia enterocolitica for HEp-2 cells. Noninvasive bacteria were not endocytosed after centrifugation. The centrifugation procedure may increase the sensitivity of testing for bacterial invasiveness in cell culture without causing false-positive results.

  20. Complicações vasculares pós-transplante hepático

    Directory of Open Access Journals (Sweden)

    Júlio Cezar Uili Coelho

    Full Text Available OBJETIVO: O objetivo do estudo é apresentar as complicações vasculares arteriais e venosas observadas em um serviço de transplante hepático universitário brasileiro. MÉTODOS: Os prontuários de todos os pacientes submetidos a transplante hepático no período de setembro de 1991 a janeiro de 2000 foram analisados para determinar as complicações vasculares e correlacioná-las a dados clínicos e do procedimento cirúrgico. RESULTADOS: Foram realizados 169 transplantes, sendo quatro inter vivos e nove retransplantes. Um total de 24 complicações vasculares (14,3% foi identificado em 22 pacientes (13,0%. A complicação vascular mais comum foi a trombose da artéria hepática (15 casos, seguido da trombose da veia porta (quatro casos. Complicações da veia cava inferior infra ou supra-hepática foram incomuns, ocorrendo em um total de três casos (1,8%. As complicações vasculares foram mais freqüentes nas crianças (26,06% do que em adultos (13,14% (p<0,05. Dos pacientes com trombose da artéria hepática, um foi submetido à angioplastia, um a trombectomia, oito a retransplante e cinco evoluíram para o óbito enquanto aguardavam retransplante. Dos quatro casos de trombose da veia porta, dois foram a óbito, um foi submetido à colocação percutânea de prótese e um a tratamento conservador. Os pacientes com estenose da veia porta e da veia cava inferior infra e supra-hepática foram submetidos a tratamento conservador, com boa evolução clínica. CONCLUSÕES: que as complicações vasculares são freqüentes após o transplante hepático, principalmente em crianças, e são associadas à elevada morbidade, mortalidade e retransplante.

  1. Habitat Evaluation Procedures (HEP) Report; Yakama Nation Wildlife Management Areas, Technical Report 1999-2000.

    Energy Technology Data Exchange (ETDEWEB)

    Raedeke, Kenneth; Raedeke, Dorothy

    2000-06-01

    Construction of the Dalles, Bonneville, McNary, and John Day Dams on the Columbia River by the federal government resulted in a substantial loss of riparian bottomland along the Columbia River. Impacts associated with the Mid-Columbia Projects were assessed for several wildlife species using the Habitat Evaluation Procedures (HEP) developed by the U.S. Fish and Wildlife Service (USDI-FWS 1980). The studies documented the loss of riparian habitat and established a baseline against which mitigation measures could be developed (USDI-FWS 1990 and USDE-BPA 1990). The impact assessments established a mitigation goal, a portion of which would be satisfied by the creation, restoration, and enhancement of riparian lands on tributaries to the Columbia River, including the Yakima Valley. The Yakama Nation (YN), the Northwest Power Planning Council, and the Bonneville Power Administration have agreed that the Yakama Nation would be funded to implement habitat restoration on lands within and adjacent to their reservation. Some of the targeted lands are owned by the Yakama Nation, some are trust lands, and some lands have been in private ownership. Since the early 1990s, the Yakama Nation has been in the process of assembling riparian lands into Wildlife Management Areas, and restoring natural hydrology and natural cover-types on these lands. The Northwest Power Planning Council, through the Bonneville Power Administration, has supported the program. HEP studies were performed by the Yakama Nation in 1990 (Bich et al. 1991) to establish baseline conditions and inventory wildlife habitat at the initiation of the restoration project. The 1990 HEP used a simplified version of the HEP to quantify baseline conditions. The present assessment is designed to evaluate the progress of the mitigation plan in meeting its stated goals. The 1999 HEP assessment has two distinct tasks: (1) Evaluation of the mitigation plan as currently implemented using the simplified YN HEP methodologies for

  2. Study of apoptotic mechanisms induced by all-trans retinoic acid and its 13-cis isomer on cellular lines of human hepato carcinoma Hep3B and HepG2

    International Nuclear Information System (INIS)

    Arce Vargas, Frederick

    2006-01-01

    Two cellular lines of liver cancer (Hep3B and HepG2) were incubated during different periods of time with some concentrations of two retinoic acid isomers (ATRA and 13-cis AR) and with 5-fu chemotherapeutic agents, cisplatin and paclitaxel. It was determined if these substances leaded cytotoxicity, apoptosis and if they modified the expression of different genes related to cellular death by apoptosis, in order to explain the hepatocellular carcinoma resistance to these drugs. HepG2 cells showed more resistance than Hep3B cells to 72 hours of treatment, as much ATRA as the 13-cis AR were toxic and produced apoptosis in two cellular lines. This type of cellular death seems to be mediated by a decrease in Bcl-xL concentration in Hep3B cells treated with both retinoids an increase in bax concentration in HepG2 cells treated with 13-cis AR. It were observed 3 and 8 proteolysis of procaspase in Hep3B cells, suggesting extrinsic via activation of the apoptosis, while cellular death in HepG2 cells seems to be independent of caspases. Cisplatin and paclitaxel leaded cytotoxicity to 48 hours of treatment, with significant differences between two cellular lines only in case of paclitaxel. Hep3B cells treated with cisplatin and HepG2 cells treated with paclytaxel suffered apoptosis. 5-FU produced toxicity only when it was used to high concentrations and the mechanism of cellular death induced by this agent seems to be primarily necrosis in Hep3B cells and apoptosis in HepG2. There was decrease in the Bcl-xL concentration in two cellular lines when it was treated with cisplatin and in HepG2 cells treated with 5-FU. Bax concentration there no was modified with no treatment. Activation of the 3 caspases seems to happen only in HepG2 cells with 5-FU and paclytaxel. These two agents, also, decreased the survivin concentration of HepG2 cells. Treatments of the three drugs produced an increase in the expression of this gen in Hep3B cells, which might explain partially the resistance

  3. Variability in the recognition of distinctive immunofluorescence patterns in different brands of HEp-2 cell slides

    Directory of Open Access Journals (Sweden)

    Alessandra Dellavance

    2013-06-01

    Full Text Available INTRODUCTION: Indirect immunofluorescence on HEp-2 cells is considered the gold standard for the detection of autoantibodies against cellular antigens. However, the culture conditions, cell fixation and permeabilization processes interfere directly in the preservation and spatial distribution of antigens. Therefore, one can assume that certain peculiarities in the processing of cellular substrate may affect the recognition of indirect immunofluorescence patterns associated with several autoantibodies. OBJECTIVE: To evaluate a panel of serum samples representing nuclear, nucleolar, cytoplasmic, mitotic apparatus, and chromosome plate patterns on HEp-2 cell substrates from different suppliers. MATERIALS AND METHODS: Seven blinded observers, independent from the three selected reference centers, evaluated 17 samples yielding different nuclear, nucleolar, cytoplasmic and mitotic apparatus patterns on HEp-2 cell slides from eight different brands. The slides were coded to maintain confidentiality of both brands and participating centers. RESULTS: The 17 HEp-2 cell patterns were identified on most substrates. Nonetheless, some slides showed deficit in the expression of several patterns: nuclear coarse speckled/U1-ribonucleoprotein associated with antibodies against RNP (U1RNP, centromeric protein F (CENP-F, proliferating cell nuclear antigen (PCNA, cytoplasmic fine speckled associated with anti-Jo-1 antibodies (histidyl synthetase, nuclear mitotic apparatus protein 1 (NuMA-1 and nuclear mitotic apparatus protein 2 (NuMA-2. CONCLUSION: Despite the overall good quality of the assessed HEp-2 substrates, there was considerable inconsistency in results among different commercial substrates. The variations may be due to the evaluated batches, hence generalizations cannot be made as to the respective brands. It is recommended that each new batch or new brand be tested with a panel of reference sera representing the various patterns.

  4. Habitat Evaluation Procedures (HEP) Report; Priest River Project, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    On July 6, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Priest River property, an acquisition completed by the Kalispel Tribe of Indians in 2001. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Priest River Project provides a total of 140.73 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 60.05 HUs for bald eagle, black-capped chickadee, and white-tailed deer. Grassland meadow habitat provides 7.39 HUs for Canada goose and mallard. Scrub-shrub vegetation provides 71.13 HUs for mallard, yellow warbler, and white-tailed deer. Open water habitat provides 2.16 HUs for Canada goose and mallard. The objective of using HEP at the Priest River Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  5. Habitat Evaluation Procedures (HEP) Report; West Beaver Lake Project, Technical Report 2005

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-05-01

    On September 7, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the West Beaver Lake property, an acquisition completed by the Kalispel Tribe of Indians in September 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, mallard, muskrat, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The West Beaver Lake Project provides a total of 82.69 Habitat Units (HUs) for the species evaluated. Emergent wetland habitat provides 8.80 HUs for mallard, muskrat, and Canada goose. Conifer forest habitat provides 70.33 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Open water provides 3.30 HUs for mallard, muskrat, and Canada goose. The objective of using HEP at the West Beaver Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  6. Habitat Evaluation Procedures (HEP) Report; North Eaton Lake, Technical Report 2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-11-01

    On July 6, 2005, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the North Eaton Lake property, an acquisition completed by the Kalispel Tribe of Indians in November 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The North Eaton Lake Project provides a total of 235.05 Habitat Units (HUs) for the species evaluated. Open water habitat provides 9.38 HUs for Canada goose, mallard and muskrat. Emergent wetland habitat provides 11.36 HUs for Canada goose, mallard and muskrat. Forested wetland provides 10.97 HUs for bald eagle, black-capped chickadee, mallard and white-tailed deer. Conifer forest habitat provides 203.34 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. The objective of using HEP at the North Eaton Lake Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  7. Habitat Evaluation Procedures (HEP) Report; Upper Trimble Project, Technical Report 2004-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On July 13, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Upper Trimble property, an acquisition completed by the Kalispel Tribe of Indians in March 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Upper Trimble Project provides a total of 250.67 Habitat Units (HUs) for the species evaluated. Wet meadow provides 136.92 HUs for mallard, muskrat, and Canada goose. Mixed forest habitat provides 111.88 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. Scrub-shrub vegetation provides 1.87 HUs for yellow warbler, and white-tailed deer. The objective of using HEP at the Upper Trimble Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  8. Habitat Evaluation Procedures (HEP) Report; Tacoma Creek South Project, Technical Report 2003-2005.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On July 6, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Tacoma Creek South property, an acquisition completed by the Kalispel Tribe of Indians in June 2004. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, muskrat, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Tacoma Creek South Project provides a total of 190.79 Habitat Units (HUs) for the species evaluated. Emergent wetlands provide 20.51 HUs for Canada goose, mallard, and muskrat. Grassland provides 1.65 HUs for Canada goose and mallard. Scrub-shrub vegetation provides 11.76 HUs for mallard, yellow warbler, and white-tailed deer. Conifer forest habitat provides 139.92 HUs for bald eagle, black-capped chickadee, and white-tailed deer. Deciduous forest also provides 19.15 HUs for bald eagle, black-capped chickadee, mallard, and white-tailed deer. The objective of using HEP at the Tacoma Creek South Project and other protected properties is to document the quality and quantity of available habitat for selected wildlife species. In this way, HEP provides information on the relative value of the same area at future points in time so that the effect of management activities on wildlife habitat can be quantified. When combined with other tools, the baseline HEP will be used to determine the most effective on-site management, restoration, and enhancement actions to increase habitat suitability for targeted species. The same process will be replicated every five years to quantitatively evaluate the effectiveness of management strategies in improving and maintaining habitat conditions while providing additional crediting to BPA for enhanced habitat values.

  9. Habitat Evaluation Procedures (HEP) Report : Ladd Marsh Wildlife Area, 2004-2006 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul; Wagoner, Sara

    2006-05-01

    The Regional HEP Team (RHT) and Oregon Department of Fish and Wildlife (ODFW) staff conducted a follow-up habitat evaluation procedures (HEP) analysis on the Ladd Marsh Wildlife Management Area (LMWA) in May 2005. The 2005 HEP assessment resulted in a total of 647.44 HUs, or 0.76 HUs/acre. This is an increase of 420.34 HUs (0.49 HUs/acre) over 2001 HEP survey results. The most significant increase in HUs occurred on the Wallender and Simonis parcels which increased by 214.30 HUs and 177.49 HUs respectively. Transects were established at or near 2001 HEP analysis transect locations whenever possible. ODFW staff biologists assisted the RHT re-establish transect locations and/or suggested areas for new surveys. Since 2001, significant changes in cover type acreage and/or structural conditions have occurred due to conversion of agriculture cover types to emergent wetland and grassland cover types. Agricultural lands were seeded to reestablish grasslands and wetlands were restored through active management and manipulation of extant water sources including natural stream hydrology/flood regimes and available irrigation. Grasslands increased on the Wallender parcel by 21% (65 acres), 23% (71 acres) at the Simonis site, and 39% (62 acres) at Conley Lake. The emergent wetland cover type also changed significantly increasing 60% (184 acres) at Wallender and 59% (184 acres) on the Simonis tract. Today, agriculture lands (crop and grazed pasture) have been nearly eliminated from Bonneville Power Administration (BPA) mitigation project lands located on the LMWA.

  10. Excesso de peso em pacientes submetidos ao transplante hepático

    Directory of Open Access Journals (Sweden)

    Lucilene Rezende Anastácio

    Full Text Available Esta revisão tem por objetivo descrever a incidência e prevalência de excesso de peso, sobrepeso e obesidade pós-transplante hepático e as consequências associadas a ele. Foi realizada revisão bibliográfica com consulta nas bases Medline/Pubmed, SciELO, EMBASE, LILACS com o cruzamento dos seguintes descritores: transplante hepático; sobrepeso; obesidade; ganho de peso. O excesso de peso é incidente em mais de 60% dos pacientes submetidos ao transplante hepático e as taxas de obesidade ultrapassam 20% já no primeiro ano do pós-operatório, período em que ocorre o maior ganho de peso relativo. Estudos revelaram que entre 60% e 70% dos pacientes submetidos ao transplante de fígado apresentam excesso de peso após o terceiro ano e quase 90% deles com obesidade abdominal. Os fatores associados são os mais variados, dentre os quais se destacam maior idade, história familiar de excesso de peso, excesso de peso anterior à doença hepática, dentre outros. A contribuição da medicação imunossupressora ainda permanece controversa. Dentre as consequências do excesso de peso estão a esteatose hepática, esteatohepatite, diabete melito, hipertensão, dislipidemias, doenças cardiovasculares e morte.

  11. Vascularização na cirrose hepática: estudo imunoistoquímico baseado em necropsias

    OpenAIRE

    Maeda,Mariane de Fátima Yukie; Silva,Camilla Duarte; Harima,Leila Suemi; Silva,Luiz Fernando Ferraz da; Ctenas,Bruno; Alves,Venâncio Avancini Ferreira

    2008-01-01

    RACIONAL: O processo patológico mais discutido na gênese da cirrose hepática é a fibrose progressiva, porém alterações na vasculatura do órgão têm sido apontadas como elementos fundamentais na fisiopatologia da doença e de suas complicações, como hipertensão portal, insuficiência hepática e carcinoma hepatocelular. OBJETIVO: Avaliar a densidade microvascular em 35 casos de necropsias de pacientes com cirrose hepática mediante pesquisa imunoistoquímica do marcador endotelial CD34 a fim de comp...

  12. Lipidosis hepática asociada con malnutrición y preñez en dos perras

    OpenAIRE

    Giordano, Andrea; Arias, Daniel Osvaldo; Araus, S.; Cohen, M.; Gobello, Cristina

    2004-01-01

    En algunos países latinoamericanos se observó que la ascites ocurre en diferentes momentos post parto en perras alimentadas con dietas hipoproteicas. El objetivo de este articulo fue describir 2 casos de perras con ascitis asociada a lipidosis hepática desarrollada después del parto. Las muestras de sangre evidenciaron anormarmalidades compatibles con enfermedad hepática. La paracentesis abdominal reveló un trasudado aséptico y la ultrasonografía hiperecogenicidad hepática. Se arribó al diagn...

  13. Liver transplantion in a patient with rapid onset parkinsonism-dementia complex induced by manganism secondary to liver failure Transplante hepático em um paciente com complexo parkinsonismo-demência rapidamente progressivo induzido por manganismo devido a insuficiência hepática

    Directory of Open Access Journals (Sweden)

    Giorgio Fabiani

    2007-09-01

    Full Text Available Bilateral and symmetric globus-pallidus hyperintensities are observed on T1-weighted MRI in most of the patients with chronic liver failure, due to manganese accumulation. We report a 53-year-old man, with rapid onset parkinsonism-dementia complex associated with accumulation of manganese in the brain, secondary to liver failure. A brain MRI was performed and a high signal on T1-weighted images was seen on globus-pallidus, as well as on T2-weighted images on the hemispheric white-matter. He was referred to a liver-transplantation. The patient passed away on the seventh postoperative day. Our findings support the concept of the toxic effects of manganese on the globus-pallidus. The treatment of this form of parkinsonism is controversial and liver-transplantation should not be considered as first line treatment but as an alternative one.Hiperintesidades simétricas e bilaterais dos gânglios da base são observadas em imagens de ressonância magnética encefálica (RM ponderadas em T1 na maioria dos pacientes com insuficiência hepática crônica devidas ao acúmulo de manganês. Nós relatamos o caso de um homem, com 53 anos de idade, com um complexo parkinsonismo-demência rapidamente progressivo associado com o acúmulo de manganês no cérebro, secundariamente a insuficiência hepática. Uma RM encefálica foi realizada e foram observadas imagens hiperintensas/hipersinal nas imagens ponderadas em T1 no globo pálido e, também, na substância branca dos hemisférios cerebrais ponderadas em T2. Devido à falta de resposta ao tratamento clinico optamos pelo transplante hepático. O paciente faleceu no 7º dia de PO. Nossos achados corroboram o conceito dos efeitos tóxicos do manganês nos gânglios da base/globo pálido. O tratamento desta forma de parkinsonismo é controverso e o transplante hepático não deverá ser considerada uma opção terapêutica de primeira linha, porém como um tratamento alternativo considerando-se os riscos

  14. Simplified Virtualization in a HEP/NP Environment with Condor

    International Nuclear Information System (INIS)

    Strecker-Kellogg, W; Caramarcu, C; Hollowell, C; Wong, T

    2012-01-01

    In this work we will address the development of a simple prototype virtualized worker node cluster, using Scientific Linux 6.x as a base OS, KVM and the libvirt API for virtualization, and the Condor batch software to manage virtual machines. The discussion in this paper provides details on our experience with building, configuring, and deploying the various components from bare metal, including the base OS, creation and distribution of the virtualized OS images and the integration of batch services with the virtual machines. Our focus was on simplicity and interoperability with our existing architecture.

  15. DeepJet: a portable ML environment for HEP

    CERN Document Server

    CERN. Geneva; Mehta, Swapneel Sundeep

    2018-01-01

    In this presentation we will detail the evolution of the DeepJet python environment. Initially envisaged to support the development of the namesake jet flavour tagger in CMS, DeepJet has grown to encompass multiple purposes within the collaboration. The presentation will describe the major features the environment sports: simple out-of-memory training with a multi-treaded approach to maximally exploit the hardware acceleration, simple and streamlined I/O to help bookkeeping of the developments, and finally docker image distribution, to simplify the deployment of the whole ecosystem on multiple datacenters. The talk will also cover future development, mainly aimed at improving user experience.

  16. RNA interference mediated inhibition of dengue virus multiplication and entry in HepG2 cells.

    Directory of Open Access Journals (Sweden)

    Mohammed Abdelfatah Alhoot

    Full Text Available BACKGROUND: Dengue virus-host cell interaction initiates when the virus binds to the attachment receptors followed by endocytic internalization of the virus particle. Successful entry into the cell is necessary for infection initiation. Currently, there is no protective vaccine or antiviral treatment for dengue infection. Targeting the viral entry pathway has become an attractive therapeutic strategy to block infection. This study aimed to investigate the effect of silencing the GRP78 and clathrin-mediated endocytosis on dengue virus entry and multiplication into HepG2 cells. METHODOLOGY/PRINCIPAL FINDINGS: HepG2 cells were transfected using specific siRNAs to silence the cellular surface receptor (GRP78 and clathrin-mediated endocytosis pathway. Gene expression analysis showed a marked down-regulation of the targeted genes (87.2%, 90.3%, and 87.8% for GRP78, CLTC, and DNM2 respectively in transfected HepG2 cells when measured by RT-qPCR. Intracellular and extracellular viral RNA loads were quantified by RT-qPCR to investigate the effect of silencing the attachment receptor and clathrin-mediated endocytosis on dengue virus entry. Silenced cells showed a significant reduction of intracellular (92.4% and extracellular viral RNA load (71.4% compared to non-silenced cells. Flow cytometry analysis showed a marked reduction of infected cells (89.7% in silenced HepG2 cells compared to non-silenced cells. Furthermore, the ability to generate infectious virions using the plaque assay was reduced 1.07 log in silenced HepG2 cells. CONCLUSIONS/SIGNIFICANCE: Silencing the attachment receptor and clathrin-mediated endocytosis using siRNA could inhibit dengue virus entry and multiplication into HepG2 cells. This leads to reduction of infected cells as well as the viral load, which might function as a unique and promising therapeutic agent for attenuating dengue infection and prevent the development of dengue fever to the severe life-threatening DHF or DSS

  17. Influencia de la lesión hepática y de la actividad alcohol deshidrogenasa y aldehido deshidrogenasa hepáticas en el metabolismo del etanol

    OpenAIRE

    Panés Díaz, Julià

    1989-01-01

    A) OBJETIVOSLos principales objetivos de la tesis han sido: 1) analizar el metabolismo hepático del etanol en los alcohólicos crónicos, 2) la producción de acetaldehido, 3) la actividad e isoenzimas de la alcohol deshidrogenasa (ADH) y aldehído deshidrogenasa (ALDH) hepáticas, y 4) la relación de cada uno de estos factores con el alcoholismo y el grado de lesión hepática.B) PACIENTESLa ADH y ALDH hepáticas se han estudiado en 84 pacientes, 60 eran alcohólicos (5 con hígado normal, 9 esteatosi...

  18. O desempenho da tomografia computadorizada na quantificação da esteatose hepática

    OpenAIRE

    Thiago Dieb Ristum Vieira

    2010-01-01

    INTRODUÇÃO: A esteatose hepática é uma doença metabólica caracterizada por acúmulo de gordura nos hepatócitos. Dentre suas causas, destacam-se o alcoolismo crônico, a obesidade, o diabetes mellitus do tipo II e a síndrome metabólica. O interesse pela detecção e quantificação da esteatose hepática tem crescido nos últimos anos devido à elevação da sua prevalência e aos avanços da técnica cirúrgica hepática. A quantificação da infiltração gordurosa hepática é importante para o acompanhamento do...

  19. Dopplerfluxometria da veia hepática em pacientes com esteatose não alcoólica

    OpenAIRE

    Borges, Valéria Ferreira de Almeida e; Diniz, Angélica Lemos Debs; Cotrim, Helma Pinchemel; Rocha, Haroldo Luis Oliva Gomes; Salomão, Frederico Chaves

    2011-01-01

    OBJETIVO: Avaliar a correlação entre a dopplerfluxometria da veia hepática direita e o grau de esteatose, inflamação e fibrose à biópsia na doença hepática gordurosa não alcoólica. MATERIAIS E MÉTODOS: Foi realizada ultrassonografia com Doppler em 80 pacientes, sendo 40 portadores de doença hepática gordurosa não alcoólica, também submetidos à biópsia. Quarenta controles normais saudáveis, sem fatores risco para doença hepática gordurosa não alcoólica foram submetidos a ultrassonografia com D...

  20. Specific binding of tubeimoside-2 with proteins in hepatocarcinoma HepG2 cells: investigation by molecular spectroscopy

    Science.gov (United States)

    Yang, Sun; Shi-Sheng, Sun; Ying-Yong, Zhao; Jun, Fan

    2012-07-01

    In this study, we compared different binding interactions of TBMS2 with proteins both in hepatocarcinoma HepG2 cells and in normal embryo hepatic L02 cells by using fluorescence, absorption, and CD spectroscopy. The fluorescence data revealed that the fluorescence intensity of proteins in the HepG2 and L02 cells decreased in the presence of TBMS2 by 30.79% and 12.01%, respectively. Binding constants and thermodynamic parameters were obtained for systems of TBMS2 with the two kinds of cell proteins. The results indicated that HepG2 cell proteins had a higher TBMS2 binding activity than those in the L02 cells. Analysis of the TBMS2 cytotoxic activities showed that TBMS2 could selectively induce apoptosis of HepG2 cells by binding to them, while its apoptotic effect on L02 cells was relatively weaker.

  1. Differential genomic effects on signaling pathways by two different CeO2 nanoparticles in HepG2 cells

    Data.gov (United States)

    U.S. Environmental Protection Agency — Differential genomic effects on signaling pathways by two different CeO2 nanoparticles in HepG2 cells. This dataset is associated with the following publication:...

  2. Prevalência da síndrome hepatopulmonar em candidatos a transplante hepático

    OpenAIRE

    PAROLIN Mônica Beatriz; COELHO Júlio Cézar Uili; PUCCINELLI Vanessa; SCHULZ Gustavo J.; SOUZA Admar Moraes de; BARROS João Adriano de

    2002-01-01

    Racional - A síndrome hepatopulmonar caracteriza-se pela presença de hipoxemia arterial resultante de dilatações vasculares intra-pulmonares em portadores de doença hepática crônica e/ou hipertensão portal. A síndrome, que resulta em hipoxemia arterial por vezes grave, pode alcançar completa resolução após o transplante hepático. Objetivo - Determinar a prevalência da síndrome hepatopulmonar em portadores de cirrose hepática, candidatos a transplante de fígado no Serviço de Transplante Hepáti...

  3. Habitat Evaluation Procedures (HEP) Report : Priest River, 2004-2005 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Entz, Ray

    2005-02-01

    On July 6, 2004, the Habitat Evaluation Procedure (HEP) was used to determine baseline habitat suitability on the Priest River property, an acquisition completed by the Kalispel Tribe of Indians in 2001. Evaluation species and appropriate models include bald eagle, black-capped chickadee, Canada goose, mallard, yellow warbler, and white-tailed deer. Habitat Suitability Index (HSI) values were visually estimated and agreed upon by all HEP team members. The Priest River Project provides a total of 105.41 Habitat Units (HUs) for the species evaluated. Conifer forest habitat provides 26.95 HUs for bald eagle, black-capped chickadee, and white-tailed deer. Grassland habitat provides 23.78 HUs for Canada goose and mallard. Scmb-shrub vegetation provides 54.68 HUs for mallard, yellow warbler, and white-tailed deer.

  4. Many-core applications to online track reconstruction in HEP experiments

    Energy Technology Data Exchange (ETDEWEB)

    Amerio, S. [Padua U.; Bastieri, D. [Padua U.; Corvo, M. [Padua U.; Gianelle, A. [Padua U.; Ketchum, W. [Los Alamos; Liu, T. [Fermilab; Lonardo, A. [INFN, Rome; Lucchesi, D. [Padua U.; Poprocki, S. [Cornell U.; Rivera, R. [Fermilab; Tosoratto, L. [INFN, Rome; Vicini, P. [INFN, Rome; Wittich, P. [Cornell U.

    2013-11-02

    Interest in parallel architectures applied to real time selections is growing in High Energy Physics (HEP) experiments. In this paper we describe performance measurements of Graphic Processing Units (GPUs) and Intel Many Integrated Core architecture (MIC) when applied to a typical HEP online task: the selection of events based on the trajectories of charged particles. We use as benchmark a scaled-up version of the algorithm used at CDF experiment at Tevatron for online track reconstruction – the SVT algorithm – as a realistic test-case for low-latency trigger systems using new computing architectures for LHC experiment. We examine the complexity/performance trade-off in porting existing serial algorithms to many-core devices. Measurements of both data processing and data transfer latency are shown, considering different I/O strategies to/from the parallel devices.

  5. CERN Library | Book presentation and discussion round: "HEP - Still an unfeminine profession" | 19 May | Council Chamber

    CERN Multimedia

    CERN Library

    2016-01-01

    Book presentation and discussion round: "HEP - Still an unfeminine profession" with Mary K. Gaillard.   “HEP – still an unfeminine profession?” Book presentation by Mary K Gaillard, followed by a discussion round with the author and Valerie Gibson (LHCb) Thursday, 19 May 2016 from 4 p.m. (coffee will be served at 3:30 p.m.) CERN Council Chamber Find more information and registration here. We hope to see many of you there! There will also be a Theory seminar by Mary K. Gaillard on "Supergravity at one loop, anomalies and applications to string phenomenology" - Wednesday, 18 May at 2 p.m. in the TH conference room.

  6. The future of commodity computing and many-core versus the interests of HEP software

    CERN Multimedia

    CERN. Geneva

    2012-01-01

    As the mainstream computing world has shifted from multi-core to many-core platforms, the situation for software developers has changed as well. With the numerous hardware and software options available, choices balancing programmability and performance are becoming a significant challenge. The expanding multiplicative dimensions of performance offer a growing number of possibilities that need to be assessed and addressed on several levels of abstraction. This paper reviews the major tradeoffs forced upon the software domain by the changing landscape of parallel technologies – hardware and software alike. Recent developments, paradigms and techniques are considered with respect to their impact on the rather traditional HEP programming models. Other considerations addressed include aspects of efficiency and reasonably achievable targets for the parallelization of large scale HEP workloads.

  7. Software R&D for Next Generation of HEP Experiments, Inspired by Theano

    CERN Multimedia

    CERN. Geneva

    2015-01-01

    In the next decade, the frontiers of High Energy Physics (HEP) will be explored by three machines: the High Luminosity Large Hadron Collider (HL-LHC) in Europe, the Long Base Neutrino Facility (LBNF) in the US, and the International Linear Collider (ILC) in Japan. These next generation experiments must address two fundamental problems in the current generation of HEP experimental software: the inability to take advantage and adapt to the rapidly evolving processor landscape, and the difficulty in developing and maintaining increasingly complex software systems by physicists. I will propose a strategy, inspired by the automatic optimization and code generation in Theano, to simultaneously address both problems. I will describe three R&D projects with short-term physics deliverables aimed at developing this strategy. The first project is to develop maximally sensitive General Search for New Physics at the LHC by applying the Matrix Element Method running GPUs of HPCs. The second is to classify and reconstru...

  8. Mapping modern software process engineering techniques onto an HEP development environment

    CERN Document Server

    Wellisch, J P

    2003-01-01

    One of the most challenging issues faced in HEP in recent years is the question of how to capitalise on software development and maintenance experience in a continuous manner. To capitalise means in our context to evaluate and apply new process technologies as they arise, and to further evolve technologies already widely in use. It also implies the definition and adoption of standards. The CMS off- line software improvement effort aims at continual software quality improvement, and continual improvement in the efficiency of the working environment with the goal to facilitate doing great new physics. To achieve this, we followed a process improvement program based on ISO-15504, and Rational Unified Process. This experiment in software process improvement in HEP has been progressing now for a period of 3 years. Taking previous experience from ATLAS and SPIDER into account, we used a soft approach of continuous change within the limits of current culture to create of de facto software process standards within th...

  9. Summary of the Control System Cyber-Security (CS)2/HEP Workshop

    CERN Document Server

    Lüders, S

    2007-01-01

    Over the last few years modern accelerator and experiment control systems have increasingly been based on commercial-off-the-shelf products (VME crates, PLCs, SCADA systems, etc.), on Windows or Linux PCs, and on communication infrastructures using Ethernet and TCP/IP. Despite the benefits coming with this (r)evolution, new vulnerabilities are inherited, too: Worms and viruses spread within seconds via the Ethernet cable, and attackers are becoming interested in control systems. Unfortunately, control PCs cannot be patched as fast as office PCs. Even worse, vulnerability scans at CERN using standard IT tools have shown that commercial automation systems lack fundamental security precautions: Some systems crashed during the scan, others could easily be stopped or their process data be altered. The (CS)2/HEP workshop held the weekend before ICALEPCS2007 was intended to present, share, and discuss countermeasures deployed in HEP laboratories in order to secure control systems. This presentation will give a summa...

  10. The role of scientific middleware in the future of HEP computing

    CERN Multimedia

    CERN. Geneva

    2004-01-01

    In the 18 months since the CHEP03 meeting in San Diego, the HEP community deployed the current generation of grid technologies in a veracity of settings. Legacy software as well as recently developed applications was interfaced with middleware tools to deliver end-to-end capabilities to HEP experiments in different stages of their life cycles. In a series of data challenges, reprocessing efforts and data distribution activities the community demonstrated the benefits distributed computing can offer and the power a range of middleware tools can deliver. After running millions of jobs, moving tera-bytes of data, creating millions of files and resolving hundreds of bug reports, the community also exposed the limitations of these middleware tools. As we move to the next level of challenges, requirements and expectations, we must also examine the methods and procedures we employ to develop, implement and maintain our common suite of middleware tools. The talk will focus on the role common middleware ...

  11. Abordaje transtorácico para la hidatidosis pulmonar derecha y hepática concomitante

    OpenAIRE

    Jans B,Jaime; Talma R,María J; Almonacid R,Mario; Herrera H,Mauricio

    2013-01-01

    Introducción: La hidatidosis pulmonar y hepática concomitante se presenta en el 4 a 33% de los casos. Cuando la afección está localizada en el pulmón derecho y el domo hepático la resolución quirúrgica en un tiempo es posible. Objetivo: Describir el abordaje transtorácico-transfrénico y revisar la literatura disponible. Material y Métodos: Se presentan dos casos clínicos resueltos quirúrgicamente en nuestro centro a través de toracotomía posterolateral derecha y frenotomía en un tiempo, con e...

  12. Design and evaluation of a hybrid storage system in HEP environment

    Science.gov (United States)

    Xu, Qi; Cheng, Yaodong; Chen, Gang

    2017-10-01

    Nowadays, the High Energy Physics experiments produce a large amount of data. These data are stored in mass storage systems which need to balance the cost, performance and manageability. In this paper, a hybrid storage system including SSDs (Solid-state Drive) and HDDs (Hard Disk Drive) is designed to accelerate data analysis and maintain a low cost. The performance of accessing files is a decisive factor for the HEP computing system. A new deployment model of Hybrid Storage System in High Energy Physics is proposed which is proved to have higher I/O performance. The detailed evaluation methods and the evaluations about SSD/HDD ratio, and the size of the logic block are also given. In all evaluations, sequential-read, sequential-write, random-read and random-write are all tested to get the comprehensive results. The results show the Hybrid Storage System has good performance in some fields such as accessing big files in HEP.

  13. Roof Reconstruction from Airborne Laser Scanning Data Based on Image Processing Methods

    Science.gov (United States)

    Goebbels, S.; Pohle-Fröhlich, R.

    2016-06-01

    The paper presents a new data-driven approach to generate CityGML building models from airborne laser scanning data. The approach is based on image processing methods applied to an interpolated height map and avoids shortcomings of established methods for plane detection like Hough transform or RANSAC algorithms on point clouds. The improvement originates in an interpolation algorithm that generates a height map from sparse point cloud data by preserving ridge lines and step edges of roofs. Roof planes then are detected by clustering the height map's gradient angles, parameterizations of planes are estimated and used to filter out noise around ridge lines. On that basis, a raster representation of roof facets is generated. Then roof polygons are determined from region outlines, connected to a roof boundary graph, and simplified. Whereas the method is not limited to churches, the method's performance is primarily tested for church roofs of the German city of Krefeld because of their complexity. To eliminate inaccuracies of spires, contours of towers are detected additionally, and spires are rendered as solids of revolution. In our experiments, the new data-driven method lead to significantly better building models than the previously applied model-driven approach.

  14. Wind Spires as an Alternative Energy Source

    Energy Technology Data Exchange (ETDEWEB)

    Majid Rashidi, Ph.D., P.E.

    2012-10-30

    This report discloses the design and development of an innovative wind tower system having an axisymmetric wind deflecting structure with a plurality of symmetrically mounted rooftop size wind turbines near the axisymmetric structure. The purpose of the wind deflecting structure is to increase the ambient wind speed that in turn results in an overall increase in the power capacity of the wind turbines. Two working prototypes were constructed and installed in the summer of 2009 and 2012 respectively. The system installed in the Summer of 2009 has a cylindrical wind deflecting structure, while the tower installed in 2012 has a spiral-shape wind deflecting structure. Each tower has 4 turbines, each rated at 1.65 KW Name-Plate-Rating. Before fabricating the full-size prototypes, computational fluid dynamic (CFD) analyses and scaled-down table-top models were used to predict the performance of the full-scale models. The performance results obtained from the full-size prototypes validated the results obtained from the computational models and those of the scaled-down models. The second prototype (spiral configuration) showed at a wind speed of 11 miles per hour (4.9 m/s) the power output of the system could reach 1,288 watt, when a typical turbine installation, with no wind deflecting structure, could produce only 200 watt by the same turbines at the same wind speed. At a wind speed of 18 miles per hour (8 m/sec), the spiral prototype produces 6,143 watt, while the power generated by the same turbines would be 1,412 watt in the absence of a wind deflecting structure under the same wind speed. Four US patents were allowed, and are in print, as the results of this project (US 7,540,706, US 7,679,209, US 7,845,904, and US 8,002,516).

  15. Aglycemia keeps mitochondrial oxidative phosphorylation under hypoxic conditions in HepG2 cells

    Czech Academy of Sciences Publication Activity Database

    Plecitá-Hlavatá, Lydie; Ježek, Jan; Ježek, Petr

    2015-01-01

    Roč. 47, č. 6 (2015), s. 467-476 ISSN 0145-479X R&D Projects: GA ČR(CZ) GAP302/10/0346; GA ČR(CZ) GA13-02033S; GA MŠk(CZ) LH11055 Institutional support: RVO:67985823 Keywords : cancer mitochondria * non-canonical response to hypoxia * hypoxia- inducible factor * glutaminolysis * HepG2 cells Subject RIV: ED - Physiology Impact factor : 2.080, year: 2015

  16. Prevalencia y factores de riesgo asociados a la Fasciola hepática en bovinos

    OpenAIRE

    Pacheco Deleg, Sandra Marcela

    2017-01-01

    El trabajo investigativo consiste en determinar la prevalencia y factores de riesgo asociados a la Fasciola hepática en bovinos localizados en la parroquia Cumbe, tomando en cuenta variables como: sexo, edad y condiciones de humedad edáfica con el fin de crear datos que afiancen el conocimiento científico. The investigative work consists in determining the prevalence and risk factors associated with Fasciola hepatic in cattle located in the Cumbe parish, taking into account variables such ...

  17. Synergistic cytotoxicity and mechanism of caffeine and lysozyme on hepatoma cell line HepG2

    Science.gov (United States)

    Yang, Hongchao; Li, Jingjuan; Cui, Lin; Ren, Yanqing; Niu, Liying; Wang, Xinguo; Huang, Yun; Cui, Lijian

    2018-03-01

    The influences of caffeine, lysozyme and the joint application of them on the hepatoma cell line HepG2 proliferation inhibition and cell apoptosis were observed by 3-(4, 5-dimethyl-2-thiazyl)-2, 5-diphenyl-2H-tetrazolium bromide assay and Hoechst 33342, which showed the proliferation inhibition rate of the joint application on HepG2 cells was 47.21%, significantly higher than caffeine or lysozyme, and the joint application promoted the apoptosis of HepG2 cells obviously. Van't Hoff classical thermodynamics formula, the Föster theory of non-radiation energy transfer and fluorescence phase diagram were used to manifest that the process of lysozyme binding to caffeine followed a two-state model, which was spontaneous at low temperature driven by enthalpy change, and the predominant intermolecular force was hydrogen bonding or Van der Waals force to stabilize caffeine-lysozyme complex with the distance 5.86 nm. The attenuated total reflection-Fourier transform infrared spectra indicated that caffeine decreased the relative contents of α-helix and β-turn, which inferred the structure of lysozyme tended to be "loose". Synchronous fluorescence spectra and ultraviolet spectra supported the above conclusion. The amino acid residues in the cleft of lysozyme were exposed and electropositivity was increased attributing to the loose structure, which were conducive to increasing caffeine concentration on the HepG2 cell surface by electrostatic interaction to show synergistic effect. The great quantities of microvilli on the liver cancer cell membrane surface, is beneficial for the lysozyme-caffeine compound to aggregate on cell surface to increase the concentration of caffeine to play stronger physiological role by electrostatic effect.

  18. Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells

    OpenAIRE

    Voigt, Jörn; Krause, Christopher; Rohwäder, Edda; Saschenbrecker, Sandra; Hahn, Melanie; Danckwardt, Maick; Feirer, Christian; Ens, Konstantin; Fechner, Kai; Barth, Erhardt; Martinetz, Thomas; Stöcker, Winfried

    2012-01-01

    Indirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF incubation in the market, the automation of result evaluation is still in its infancy. Therefore, the E...

  19. Cacao polyphenols influence the regulation of apolipoprotein in HepG2 and Caco2 cells.

    Science.gov (United States)

    Yasuda, Akiko; Natsume, Midori; Osakabe, Naomi; Kawahata, Keiko; Koga, Jinichiro

    2011-02-23

    Cocoa powder is rich in polyphenols, such as catechins and procyanidins, and has been shown to inhibit low-density lipoprotein (LDL) oxidation and atherogenesis in a variety of models. Human studies have also shown daily intake of cocoa increases plasma high-density lipoprotein (HDL) and decreases LDL levels. However, the mechanisms responsible for these effects of cocoa on cholesterol metabolism have yet to be fully elucidated. The present study investigated the effects of cacao polyphenols on the production of apolipoproteins A1 and B in human hepatoma HepG2 and intestinal Caco2 cell lines. The cultured HepG2 cells or Caco2 cells were incubated for 24 h in the presence of cacao polyphenols such as (-)-epicatechin, (+)-catechin, procyanidin B2, procyanidin C1, and cinnamtannin A2. The concentration of apolipoproteins in the cell culture media was quantified using an enzyme-linked immunoassay, and the mRNA expression was quantified by RT-PCR. Cacao polyphenols increased apolipoprotein A1 protein levels and mRNA expression, even though apolipoprotein B protein and the mRNA expression were slightly decreased in both HepG2 cells and Caco2 cells. In addition, cacao polyphenols increased sterol regulatory element binding proteins (SREBPs) and activated LDL receptors in HepG2 cells. These results suggest that cacao polyphenols may increase the production of mature form SREBPs and LDL receptor activity, thereby increasing ApoA1 and decreasing ApoB levels. These results elucidate a novel mechanism by which HDL cholesterol levels become elevated with daily cocoa intake.

  20. Habitat Evaluation Procedures (HEP) Report : Oxbow Conservation Area, 2002-2005 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Cochran, Brian

    2005-02-01

    This Habitat Evaluation Procedure (HEP) study was performed to determine baseline habitat units on the Oxbow Conservation Area in Grant County, Oregon. The evaluation is a required part of the Memorandum of Agreement between the Confederated Tribes of the Warm Springs and Bonneville Power Administration (BPA) relating to the acquisition and management of the Oxbow Conservation Area. The HEP team was comprised of individuals from the Washington Department of Fish and Wildlife and the Confederated Tribes of the Warm Springs Reservation of Oregon. The survey was conducted using the following HEP evaluation models for key species: black-capped chickadee (Poecile atricapilla), mallard (Anas platyrhynchos), mink (Mustela vison), western meadowlark (Sturnella neglecta), white-tailed deer (Odocoileus virginiana), and yellow warbler (Dendroica petechia). Cover types used in this survey were conifer forest, irrigated meadow, riparian meadow, upland meadow, riparian shrub, upland shrub, and mine tailings. The project generated 701.3 habitat units for mitigation crediting purposes. Results for each HEP species are: (1) Black-capped chickadee habitat was good, with only isolated areas lacking snags or having low tree canopy cover. (2) Mallard habitat was poor in upland meadows and marginal elsewhere due to a lack of herbaceous/shrub cover and low herbaceous height. (3) Mink habitat was good, limited only by the lack of the shrub component. (4) Western meadowlark habitat was marginal in upland meadow and mine tailing cover types and good in irrigated meadow. Percent cover of grass and height of herbaceous variables were limiting factors. (5) White-tailed deer habitat was marginal due to relatively low tree canopy cover, reduced shrub cover, and limited browse diversity. (6) Yellow Warbler habitat was marginal due to less than optimum shrub height and the lack of hydrophytic shrubs. General ratings (poor, marginal, etc.) are described in the introduction section.

  1. The CMSSW benchmarking suite: Using HEP code to measure CPU performance

    Science.gov (United States)

    Benelli, G.; CMS Offline Computing Projects

    2010-04-01

    The demanding computing needs of the CMS experiment require thoughtful planning and management of its computing infrastructure. A key factor in this process is the use of realistic benchmarks when assessing the computing power of the different architectures available. In recent years a discrepancy has been observed between the CPU performance estimates given by the reference benchmark for HEP computing (SPECint [1]) and actual performances of HEP code. Making use of the CPU performance tools from the CMSSW performance suite, comparative CPU performance studies have been carried out on several architectures. A benchmarking suite has been developed and integrated in the CMSSW framework, to allow computing centers and interested third parties to benchmark architectures directly with CMSSW. The CMSSW benchmarking suite can be used out of the box, to test and compare several machines in terms of CPU performance and report with the wanted level of detail the different benchmarking scores (e.g. by processing step) and results. In this talk we describe briefly the CMSSW software performance suite, and in detail the CMSSW benchmarking suite client/server design, the performance data analysis and the available CMSSW benchmark scores. The experience in the use of HEP code for benchmarking will be discussed and CMSSW benchmark results presented.

  2. Habitat Evaluation Procedures (HEP) Report; Burlington Bottoms, Technical Report 1993-2003.

    Energy Technology Data Exchange (ETDEWEB)

    Beilke, Susan

    1993-08-01

    Burlington Bottoms, consisting of approximately 417 acres of riparian and wetland habitat, was purchased by the Bonneville Power Administration in November 1991. The site is located approximately 1/2 mile north of the Sauvie Island Bridge (T2N R1W Sections 20, 21), and is bound on the east side by Multnomah Channel and on the west side by the Burlington Northern Railroad right-of-way and U.S. Highway 30 (Figures 1 and 2). Wildlife habitat values resulting from the purchase of this site will contribute toward the goal of mitigating for habitat lost as outlined in the Columbia and Willamette River Basin's Fish and Wildlife Program and Amendments. Under this Program, mitigation goals were developed as a result of the loss of wildlife habitat due to the development and operation of Federal hydro-electric facilities in the Columbia and Willamette River Basins. In 1993, an interdisciplinary team was formed to develop and implement quantitative Habitat Evaluation Procedures (HEP) to document the value of various habitats at Burlington Bottoms. Results of the HEP will be used to: (1) determine the current status and habitat enhancement potential of the site consistent with wildlife mitigation goals and objectives; and (2) develop a management plan for the area. HEP participants included; Charlie Craig, BPA; Pat Wright, Larry Rasmussen, and Ron Garst, U. S. Fish and Wildlife Service; John Christy, The Nature Conservancy; and Doug Cottam, Sue Beilke, and Brad Rawls, Oregon Department of Fish and Wildlife.

  3. Protective effects of quercetin on nicotine induced oxidative stress in 'HepG2 cells'.

    Science.gov (United States)

    Yarahmadi, Amir; Zal, Fatemeh; Bolouki, Ayeh

    2017-10-01

    Nicotine is a natural component of tobacco plants and is responsible for the addictive properties of tobacco. Nicotine has been recognized to result in oxidative stress by inducing the generation of reactive oxygen species (ROS). The purpose of this work was to estimate the hepatotoxicity effect of nicotine on viability and on antioxidant defense system in cultures of HepG2 cell line and the other hand, ameliorative effect of quercetin (Q) as an antioxidant was analyzed. Nicotine induced concentration dependent loss in HepG2 cell line viability. The results indicated that nicotine decreased activity of superoxide dismutase (SOD) and glutathione reductase (GR) and increased activities of catalase (CAT) and glutathione peroxidase (GPx) and glutathione (GSH) content in the HepG2 cells. Q significantly increased activity of SOD, GR and GSH content and decreased activity of GPX in nicotine + Q groups. Our data demonstrate that Q plays a protective role against the imbalance elicited by nicotine between the production of free radicals and antioxidant defense systems, and suggest that administration of this antioxidant may find clinical application where cellular damage is a consequence of ROS.

  4. Effects of elaidic acid in a HepG2-SF liver cell model

    DEFF Research Database (Denmark)

    Hansen, Toke Peter Krogager

    Det primære mål for dette Ph.D-studie var at identificere potentielle proteinbiomarkører for menneskelig indtagelse af elaidinsyre, hvilken er den mest almindelige transfedtsyre i fødevarer. En serum fri HepG2 celle model (HepG-SF) blev inkuberet i syv dage med elaidinsyre eller med andre...... human blodplasma. Det sekundære mål for Ph.D-studiet var at undersøge årsagerne til den specifikke cellulære respons for elaidinsyre. Det blev observeret at inkubation med elaidinsyre resulterede i at 28 % af de esterficerede fedtsyrerne i fosfolipid-fraktionen var elaidinsyre, hvilket indikerer en...... lipidmetabolismen når HepG2-SF celler blev inkuberet med elaidinsyre sammenlignet med oleinsyre eller stearinsyre. Den mest fremtrædende ændring var en opregulering af enzymer som syntetiserer kolesterol og fedtsyrer, hvilken indikerede aktivering af sterol regulatory element-binding proteins (SREBPs). Dog blev...

  5. Achieving production-level use of HEP software at the Argonne Leadership Computing Facility

    Science.gov (United States)

    Uram, T. D.; Childers, J. T.; LeCompte, T. J.; Papka, M. E.; Benjamin, D.

    2015-12-01

    HEP's demand for computing resources has grown beyond the capacity of the Grid, and these demands will accelerate with the higher energy and luminosity planned for Run II. Mira, the ten petaFLOPs supercomputer at the Argonne Leadership Computing Facility, is a potentially significant compute resource for HEP research. Through an award of fifty million hours on Mira, we have delivered millions of events to LHC experiments by establishing the means of marshaling jobs through serial stages on local clusters, and parallel stages on Mira. We are running several HEP applications, including Alpgen, Pythia, Sherpa, and Geant4. Event generators, such as Sherpa, typically have a split workload: a small scale integration phase, and a second, more scalable, event-generation phase. To accommodate this workload on Mira we have developed two Python-based Django applications, Balsam and ARGO. Balsam is a generalized scheduler interface which uses a plugin system for interacting with scheduler software such as HTCondor, Cobalt, and TORQUE. ARGO is a workflow manager that submits jobs to instances of Balsam. Through these mechanisms, the serial and parallel tasks within jobs are executed on the appropriate resources. This approach and its integration with the PanDA production system will be discussed.

  6. Downregulation of human paraoxonase 1 (PON1) by organophosphate pesticides in HepG2 cells.

    Science.gov (United States)

    Medina-Díaz, Irma Martha; Ponce-Ruiz, Néstor; Ramírez-Chávez, Bryana; Rojas-García, Aurora Elizabeth; Barrón-Vivanco, Briscia S; Elizondo, Guillermo; Bernal-Hernández, Yael Y

    2017-02-01

    Paraoxonase 1 (PON1) is a calcium-dependent esterase synthesized primarily in the liver and secreted into the plasma where it is associated with high-density lipoproteins (HDL). PON1 hydrolyzes and detoxifies some toxic metabolites of organophosphorus compounds (OPs) such as methyl parathion and chlorpyrifos. Thus, PON1 activity and expression levels are important for determining susceptibility against OPs poisoning. Some studies have demonstrated that OPs can modulate gene expression through interactions with nuclear receptors. In this study, we evaluated the effects of methyl parathion and chlorpyrifos on the modulation of PON1 in Human Hepatocellular Carcinoma (HepG2) cells by real-time PCR, PON1 activity assay, and western blot. The results showed that the treatments with methyl parathion and chlorpyrifos decreased PON1 mRNA and immunoreactive protein and increased inflammatory cytokines in HepG2 cells. The effects of methyl parathion and chlorpyrifos on the downregulation of PON1 gene expression in HepG2 cells may provide evidence of OPs cytotoxicity related to oxidative stress and an inflammatory response. A decrease in the expression of the PON1 gene may increase the susceptibility to OPs intoxication and the risk of diseases related to inflammation and oxidative stress. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 490-500, 2017. © 2016 Wiley Periodicals, Inc.

  7. Scaling HEP to Web size with RESTful protocols: The frontier example

    International Nuclear Information System (INIS)

    Dykstra, Dave

    2011-01-01

    The World-Wide-Web has scaled to an enormous size. The largest single contributor to its scalability is the HTTP protocol, particularly when used in conformity to REST (REpresentational State Transfer) principles. High Energy Physics (HEP) computing also has to scale to an enormous size, so it makes sense to base much of it on RESTful protocols. Frontier, which reads databases with an HTTP-based RESTful protocol, has successfully scaled to deliver production detector conditions data from both the CMS and ATLAS LHC detectors to hundreds of thousands of computer cores worldwide. Frontier is also able to re-use a large amount of standard software that runs the Web: on the clients, caches, and servers. I discuss the specific ways in which HTTP and REST enable high scalability for Frontier. I also briefly discuss another protocol used in HEP computing that is HTTP-based and RESTful, and another protocol that could benefit from it. My goal is to encourage HEP protocol designers to consider HTTP and REST whenever the same information is needed in many places.

  8. HepG2 human hepatocarcinomas cells sensitization by endogenous porphyrins

    Science.gov (United States)

    Vonarx-Coinsmann, Veronique; Foultier, Marie-Therese; de Brito, Leonor X.; Morlet, Laurent; Patrice, Thierry

    1995-03-01

    We assessed the ability of the human hepatocarcinoma cell line HepG2 to synthesize PpIX in vitro from exogenous ALA and analyzed ALA-induced toxicity and phototoxicity on this cell line. ALA induced a slight dose-dependent dark toxicity, with 79 and 66% cell survival respectively for ALA 50 and 100 mg/ml after 3-h incubation. Whereas the same treatment followed by laser irradiation (l equals 632 nm, 25 J/sq cm) induced dose-dependent phototoxicity, with 54 and 19% cell survival 24 h after PDT. Whatever the incubation time with ALA, a 3-h delay before light exposure was found optimal to reach a maximal phototoxicity. Photoproducts induced by porphyrin light irradiation absorbed light in the red spectral region at longer wavelengths than did the original porphyrins. The possible enhancement of PDT effects after ALA HepG2 cell incubation was investigated by irradiating cells successively with red light (l equals 632 nm) and light (l equals 650 nm). Total fluence was kept constant at 25 J/sq cm. Phototoxicity was lower when cells were irradiated for increased periods of l equals 650 nm light than with l equals 632 nm light alone. Any photoproducts involved had either a short life or were poorly photoreactive. HepG2 cells, synthesizing enzymes and precursors of endogenous porphyrin synthesis, represent a good in vitro model for experiments using ALA-PpIX-PDT.

  9. Tripterygium regelii decreases the biosynthesis of triacylglycerol and cholesterol in HepG2 cells.

    Science.gov (United States)

    Kang, Myung-Ji; Kwon, Eun-Bin; Yuk, Heung Joo; Ryu, Hyung Won; Kim, Soo-Yeon; Lee, Mi-Kyeong; Moon, Dong-Oh; Lee, Su Ui; Oh, Sei-Ryang; Lee, Hyun-Sun; Kim, Mun-Ock

    2017-12-01

    In the course of screening to find a plant material decreasing the activity of triacylglycerol and cholesterol, we identified Tripterygium regelii (TR). The methanol extract of TR leaves (TR-LM) was shown to reduce the intracellular lipid contents consisting of triacylglycerol (TG) and cholesterol in HepG2 cells. TR-LM also downregulated the mRNA and protein expression of the lipogenic genes such as SREBP-1 and its target enzymes. Consequently, TR-LM reduced the TG biosynthesis in HepG2 cells. In addition, TR-LM decreased SREBP2 and its target enzyme HMG-CoA reductase, which is involved in cholesterol synthesis. In this study, we evaluated that TR-LM attenuated cellular lipid contents through the suppression of de novo TG and cholesterol biosynthesis in HepG2 cells. All these taken together, TR-LM could be beneficial in regulating lipid metabolism and useful preventing the hyperlipidemia and its complications, in that liver is a crucial tissue for the secretion of serum lipids.

  10. In Vitro Cytotoxic Effect of Brazilian Green Propolis on Human Laryngeal Epidermoid Carcinoma (HEp-2 Cells

    Directory of Open Access Journals (Sweden)

    Michelle C. Búfalo

    2009-01-01

    Full Text Available Propolis is a sticky dark-colored material showing a very complex chemical composition that honeybees collect from plants. It has been used in folk medicine since ancient times, due to several biological properties, such as antimicrobial, anti-inflammatory, antioxidant and immunomodulatory activities, among others. Its antitumor action in vivo and in vitro has also been reported, using propolis extracts or its isolated compounds. The goal of this work was to evaluate propolis's cytotoxic action in vitro on human laryngeal epidermoid carcinoma (Hep-2 cells. These cells were incubated with different concentrations of this bee product for different time periods, and morphology and the number of viable HEp-2 cells analyzed. Data showed that propolis exhibited a cytotoxic effect in vitro against HEp-2 cells, in a dose- and time-dependent way. Propolis solvent had no effects on morphology and number of viable cells, proving that the cytotoxic effects were exclusively due to propolis components. Since humans have been using propolis for a long time, further assays will provide a better comprehension of propolis's antitumor action.

  11. Mapping modern software process engineering techniques onto an HEP development environment

    International Nuclear Information System (INIS)

    Wellisch, J.P.

    2003-01-01

    One of the most challenging issues faced in HEP in recent years is the question of how to capitalise on software development and maintenance experience in a continuous manner. To capitalise means in our context to evaluate and apply new process technologies as they arise, and to further evolve technologies already widely in use. It also implies the definition and adoption of standards. The CMS off-line software improvement effort aims at continual software quality improvement, and continual improvement in the efficiency of the working environment with the goal to facilitate doing great new physics. To achieve this, we followed a process improvement program based on ISO-15504, and Rational Unified Process. This experiment in software process improvement in HEP has been progressing now for a period of 3 years. Taking previous experience from ATLAS and SPIDER into account, we used a soft approach of continuous change within the limits of current culture to create of de facto software process standards within the CMS off line community as the only viable route to a successful software process improvement program in HEP. We will present the CMS approach to software process improvement in this process R and D, describe lessons learned, and mistakes made. We will demonstrate the benefits gained, and the current status of the software processes established in CMS off-line software

  12. Investigation of quercetin-induced HepG2 cell apoptosis-associated cellular biophysical alterations by atomic force microscopy.

    Science.gov (United States)

    Pi, Jiang; Li, Baole; Tu, Lvying; Zhu, Haiyan; Jin, Hua; Yang, Fen; Bai, Haihua; Cai, Huaihong; Cai, Jiye

    2016-01-01

    Quercetin, a wildly distributed bioflavonoid, has been proved to possess excellent antitumor activity on hepatocellular carcinoma (HCC). In the present study, the biophysical properties of HepG2 cells were qualitatively and quantitatively determined using high resolution atomic force microscopy (AFM) to understand the anticancer effects of quercetin on HCC cells at nanoscale. The results showed that quercetin could induce severe apoptosis in HepG2 cells through arrest of cell cycle and disruption of mitochondria membrane potential. Additionally, the nuclei and F-actin structures of HepG2 cells were destroyed by quercetin treatment as well. AFM morphological data showed some typical apoptotic characterization of HepG2 cells with increased particle size and roughness in the ultrastructure of cell surface upon quercetin treatment. As an important biophysical property of cells, the membrane stiffness of HepG2 cells was further quantified by AFM force measurements, which indicated that HepG2 cells became much stiffer after quercetin treatment. These results collectively suggest that quercetin can be served as a potential therapeutic agent for HCC, which not only extends our understanding of the anticancer effects of quercetin against HCC cells into nanoscale, but also highlights the applications of AFM for the investigation of anticancer drugs. © Wiley Periodicals, Inc.

  13. Substâncias vasoativas e a modulação do sistema microvascular hepático

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    Loureiro-Silva M.R.

    1999-01-01

    Full Text Available OBJETIVO. Revisão da filogênese e ontogênese hepáticas, do sistema microvascular hepático e da modulação do tônus deste sistema vascular por diferentes substâncias vasoativas. MÉTODO. Levantamento de artigos por meio do sistema MEDLINE e consulta a livros-texto. RESULTADO. Foram selecionados 52 trabalhos publicados entre 1949 e 1997, dos quais retiramos as informações a respeito de filogênese e ontogênese hepáticas, sistema microvascular hepático e mecanismos de controle do tônus vascular hepático. CONCLUSÃO. O fígado possui sistema vascular altamente especializado na promoção de mecanismos de troca entre hepatócitos e sangue. Diferentes fatores atuam continuamente sobre estruturas contrácteis deste sistema vascular adequando a perfusão do tecido hepático às necessidades homeostáticas de cada momento. O fígado é órgão eminentemente mantenedor do meio interno.

  14. Oridonin increases anticancer effects of lentinan in HepG2 human hepatoblastoma cells.

    Science.gov (United States)

    Sun, Zhiqiang; Han, Qinghe; Duan, Liwei; Yuan, Qinghai; Wang, Hui

    2018-02-01

    The aim of the present study was to investigate whether oridonin is able to increase the effects of lentinan (LNT) in HepG2 human hepatoblastoma cells by MTT, flow cytometry, reverse transcription-quantitative polymerase chain reaction and western blot analysis. The in vitro results demonstrated that 20 µg/ml of oridonin was a nontoxic concentration for L02 normal liver cells and HepG2 liver cancer cells. Furthermore, treatment with 0-200 µg/ml LNT was only able to decrease the viability of HepG2 liver cancer cells. The growth inhibitory rate of the LNT-L (100 µg/ml) treatment group was 20.7% and the rate of the LNT-H (200 µg/ml) treatment group was 54.8%. Notably, the growth inhibitory rate of the oridonin + LNT-H group was 84.3%. The highest percentage of apoptotic cells was observed in the oridonin + LNT-H group (20 µg/ml oridonin and 200 µg/ml LNT). The percentage of apoptotic cells in the oridonin + LNT-H group was significantly different from the percentage of apoptotic cells in the LNT-H (26.1%) and the LNT-L (16.8%) groups. Treatment with LNT produced an increase in caspase-3, caspase-9, Bcl-2-like protein 4, p53, p21, nuclear factor κB inhibitor-α mRNA and protein expression and a decrease in B-cell lymphoma 2 and nuclear factor-κB expression in HepG2 cells compared with untreated control cells. Treatment with a combination of oridonin and LNT-H induced a further increase in expression with the biggest differences in expression observed between the oridonin + LNT-H group and control. It was observed that treatment with oridonin was able to increase the anticancer effects of LNT in HepG2 cells. Therefore, oridonin may be used to sensitize cells to LNT.

  15. Hepatic microcirculatory failure Falência microcirculatória hepática

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    Fernando Silva Ramalho

    2006-01-01

    Full Text Available Liver ischemia has been considered a frequent problem in medical practice, and can be associated to a number of surgical and clinical situations, such as massive hepatic resections, sepsis, liver trauma, circulatory shock and liver transplantation. After restoring blood flow, the liver is further subjected to an additional injury more severe than that induced by ischemia. On account of the complexity of mechanisms related to pathophysiology of ischemia and reperfusion (I/R injury, this review deals with I/R effects on sinusoidal microcirculation, especially when steatosis is present. Alterations in hepatic microcirculation are pointed as a main factor to explain lower tolerance of fatty liver to ischemia-reperfusion insult. The employment of therapeutic strategies that interfere directly with vasoactive mediators (nitric oxide and endothelins acting on the sinusoidal perfusion seem to be determinant for the protection of the liver parenchyma against I/R. These approaches could be very suitable to take advantage of marginal specimens as fatty livers, in which the microcirculatory disarrangements hamper its employment in liver transplantation.A isquemia hepática é um problema relativamente freqüente na prática clínica, sobrevindo em situações diversas como ressecções hepáticas maciças, sepse, trauma hepático extenso, choque circulatório e transplante hepático. Durante a restauração do fluxo sanguíneo, o fígado é submetido a uma agressão adicional ainda mais intensa que aquela imposta pela isquemia. Devido à complexidade dos diversos mecanismos envolvidos na fisiopatologia da lesão por isquemia e reperfusão (I/R hepática, esta revisão se limitará a discorrer sobre os efeitos da I/R na microcirculação sinusoidal, com ênfase para as alterações microvasculares que tomam lugar no fígado esteatótico pós-isquêmico. O desarranjo microcirculatório é apontado como um importante fator para explicar a reduzida toler

  16. The Great Observatories All-Sky LIRG Survey: Herschel Image Atlas and Aperture Photometry

    Science.gov (United States)

    Chu, Jason K.; Sanders, D. B.; Larson, K. L.; Mazzarella, J. M.; Howell, J. H.; Díaz-Santos, T.; Xu, K. C.; Paladini, R.; Schulz, B.; Shupe, D.; Appleton, P.; Armus, L.; Billot, N.; Chan, B. H. P.; Evans, A. S.; Fadda, D.; Frayer, D. T.; Haan, S.; Ishida, C. M.; Iwasawa, K.; Kim, D.-C.; Lord, S.; Murphy, E.; Petric, A.; Privon, G. C.; Surace, J. A.; Treister, E.

    2017-04-01

    Far-infrared images and photometry are presented for 201 Luminous and Ultraluminous Infrared Galaxies [LIRGs: log ({L}{IR}/{L}⊙ )=11.00{--}11.99, ULIRGs: log ({L}{IR}/{L}⊙ )=12.00{--}12.99], in the Great Observatories All-Sky LIRG Survey (GOALS), based on observations with the Herschel Space Observatory Photodetector Array Camera and Spectrometer (PACS) and the Spectral and Photometric Imaging Receiver (SPIRE) instruments. The image atlas displays each GOALS target in the three PACS bands (70, 100, and 160 μm) and the three SPIRE bands (250, 350, and 500 μm), optimized to reveal structures at both high and low surface brightness levels, with images scaled to simplify comparison of structures in the same physical areas of ˜100 × 100 kpc2. Flux densities of companion galaxies in merging systems are provided where possible, depending on their angular separation and the spatial resolution in each passband, along with integrated system fluxes (sum of components). This data set constitutes the imaging and photometric component of the GOALS Herschel OT1 observing program, and is complementary to atlases presented for the Hubble Space Telescope, Spitzer Space Telescope, and Chandra X-ray Observatory. Collectively, these data will enable a wide range of detailed studies of active galactic nucleus and starburst activity within the most luminous infrared galaxies in the local universe. Based on Herschel Space Observatory observations. Herschel is an ESA space observatory with science instruments provided by the European-led Principal Investigator consortia, and important participation from NASA.

  17. Incidencia de la distomatosis hepática en los conejos de la ciudad de Lima y alrededores

    Directory of Open Access Journals (Sweden)

    Oswaldo Meneses

    1955-12-01

    Full Text Available En el lapso comprendido entre el 22 de enero y 22 de agosto de 1954, se llevó a cabo una encuesta en 538 conejos criados en Lima y alrededores tratando de determinar la incidencia de infestación por la Fasciola hepática. Se utilizó la técnica de sedimentación para el examen coprológico de las heces. Al mismo tiempo, paralelamente con la encuesta, se realizaron autopsias en conejos muertos por diversas causas con el fin de buscar F. hepática en el hígado. También se determinó la viabilidad de los huevos de F. hepática obtenidos de las heces de los conejos parasitados. De los estudios realizados se puede sacar las siguientes conclusiones: 1. Se ha verificado la presencia de Fasciola hepática en los conejos de Lima y alrededores. 2. La incidencia a F. hepática en los conejos estudiados varía entre 2.6 % y 19.7 % de acuerdo con la alimentación. 3. Son viables los huevos de F. hepática que el conejo arroja con las heces, lo que indica la posibilidad de que este animal podría actuar como reservorio de distomatosis hepática en la ciudad de Lima. 4. La técnica de sedimentación para el diagnóstico de la distomatosis en conejos ha demostrado ser superior a otras técnicas.

  18. Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells

    International Nuclear Information System (INIS)

    Yin, Wanzhong; Wang, Ping; Wang, Xin; Song, Wenzhi; Cui, Xiangyan; Yu, Hong; Zhu, Wei

    2013-01-01

    Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (0.02-0.96'µM). Microarray assays were performed to compare the microRNA and mRNA expression profiles of Hep-2 and Hep-2/v cells. Compared to Hep-2 cells, Hep-2/v cells were more resistant to chemotherapy drugs (∼45-fold more resistant to vincristine, 5.1-fold more resistant to cisplatin, and 5.6-fold more resistant to 5-fluorouracil) and had a longer doubling time (42.33±1.76 vs 28.75±1.12'h, P<0.05), higher percentage of cells in G0/G1 phase (80.98±0.52 vs 69.14±0.89, P<0.05), increased efflux of rhodamine 123 (95.97±0.56 vs 12.40±0.44%, P<0.01), and up-regulated MDR1 expression. A total of 7 microRNAs and 605 mRNAs were differentially expressed between the two cell types. Of the differentially expressed mRNAs identified, regulator of G-protein signaling 10, high-temperature requirement protein A1, and nuclear protein 1 were found to be the putative targets of the differentially expressed microRNAs identified. These findings may open a new avenue for clarifying the mechanisms responsible for MDR in laryngeal cancer

  19. Chloride intracellular channel 1 identified using proteomic analysis plays an important role in the radiosensitivity of HEp-2 cells via reactive oxygen species production.

    Science.gov (United States)

    Kim, Jae-Sung; Chang, Jong Wook; Yun, Hong Shik; Yang, Kyung Mi; Hong, Eun-Hee; Kim, Dong Hyun; Um, Hong-Duck; Lee, Kee-Ho; Lee, Su-Jae; Hwang, Sang-Gu

    2010-07-01

    The nature of the molecules underlying the radioresistance phenotype of laryngeal cancer cells remains to be established. We initially generated radioresistant laryngeal cancer cell lines from human HEp-2 cells with fractionated radiation. These RR-HEp-2 cells and isolated clones displayed more radioresistant and anti-apoptotic phenotypes than parental HEp-2 cells after radiation. Characteristics of RR-Hep-2 cell lines were confirmed by upregulation of radioresistance-related genes, such as epidermal growth factor receptor, Hsp90, and Bcl-xl. Subsequently, we examined proteome changes between HEp-2 and RR-HEp-2 cells and identified 16 proteins showing significantly altered expression levels. Interestingly, protein expression of chloride intracellular channel 1 (CLIC1) was markedly suppressed in RR-HEp-2 cells, compared with non-irradiated control cells. Suppression of CLIC1 with an indanyloxyacetic acid-94 or small interfering RNA led to radioresistance in HEp-2 cells by suppressing the radiation-induced cellular ROS level. However, ectopic overexpression of CLIC1 induced radiosensitivity in RR-HEp-2 cells via induction of ROS level after radiation, suggesting that the protein acts as a positive regulator of ROS production. Our results collectively indicate that suppression of CLIC1 contributes to acquisition of the radioresistance phenotype of laryngeal cancer cells via inhibition of ROS production, implying that this protein is an important candidate molecule for radiotherapy in radioresistant laryngeal cancer cells.

  20. CalcHEP 3.4 for collider physics within and beyond the Standard Model

    Science.gov (United States)

    Belyaev, Alexander; Christensen, Neil D.; Pukhov, Alexander

    2013-07-01

    We present version 3.4 of the CalcHEP software package which is designed for effective evaluation and simulation of high energy physics collider processes at parton level. The main features of CalcHEP are the computation of Feynman diagrams, integration over multi-particle phase space and event simulation at parton level. The principle attractive key-points along these lines are that it has: (a) an easy startup and usage even for those who are not familiar with CalcHEP and programming; (b) a friendly and convenient graphical user interface (GUI); (c) the option for the user to easily modify a model or introduce a new model by either using the graphical interface or by using an external package with the possibility of cross checking the results in different gauges; (d) a batch interface which allows to perform very complicated and tedious calculations connecting production and decay modes for processes with many particles in the final state. With this features set, CalcHEP can efficiently perform calculations with a high level of automation from a theory in the form of a Lagrangian down to phenomenology in the form of cross sections, parton level event simulation and various kinematical distributions. In this paper we report on the new features of CalcHEP 3.4 which improves the power of our package to be an effective tool for the study of modern collider phenomenology. Program summaryProgram title: CalcHEP Catalogue identifier: AEOV_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEOV_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 78535 No. of bytes in distributed program, including test data, etc.: 818061 Distribution format: tar.gz Programming language: C. Computer: PC, MAC, Unix Workstations. Operating system: Unix. RAM: Depends on process under study

  1. Metallomics Study of CdSe/ZnS Quantum Dots in HepG2 Cells.

    Science.gov (United States)

    Peng, Lu; He, Man; Chen, Beibei; Qiao, Yu; Hu, Bin

    2015-10-27

    Toxicity of quantum dots (QDs) has been a hot research concern in the past decade, and there is a lot of challenge in this field. The physicochemical characteristics of QDs can affect their toxicity, while little is known about the specific chemical form of QDs in living cells after incubation so far. In this work, speciation of four CdSe/ZnS QDs in HepG2 cells was carried out from the metallomics' point of view for the first time by using size exclusion chromatography (SEC) coupled with inductively coupled plasma-mass spectrometry (ICP-MS). On the basis of the signal of Cd, two kinds of chemical forms, named as QD-1 and QD-2, were observed in HepG2 cells incubated with CdSe/ZnS QDs. QD-1 was demonstrated to be a kind of QD-like nanoparticles, confirmed by chromatographic retention time, transmission electron microscopy (TEM) characterization, and fluorescence detection. QD-2 was demonstrated to be cadmium-metallothioneins complex (Cd-MTs) by reversed phase liquid chromatography (RPLC) synchronously coupled with ICP-MS and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) analysis. Meanwhile, speciation of QDs in HepG2 cells incubated with different conditions was analyzed. With the variation of QDs incubation concentration/time, and elimination time, the species of QD-1 and QD-2 were also observed without other obvious species, and both the amount of QD-1 and QD-2 increased with incubation concentration and time. The obtained results provide valuable information and a strategy for the study of existing chemical form of QDs, greatly benefiting the understanding of QDs toxicity in living cells.

  2. Fasudil inhibits proliferation and migration of Hep-2 laryngeal carcinoma cells

    Directory of Open Access Journals (Sweden)

    Zhang X

    2018-02-01

    Full Text Available Xiaowen Zhang,1 Nan Wu2 1Medical Research Center, Shengjing Hospital of China Medical University, Shenyang, China; 2The Core Laboratory for Public Health Science and Practice, The First Affiliated Hospital of China Medical University, Shenyang, China Background: Rho-kinase signal pathway is a new target for cancer therapy. Fasudil, a selective Rho-kinase inhibitor, is found to exert antitumor effects on several types of cancer, but whether fasudil has antitumor effects on laryngeal carcinoma is still unknown. The aim of this study was to determine the effects of fasudil on laryngeal carcinoma and explore the underlying molecular mechanisms in this process. Methods: After treatment with fasudil, changes in biological behaviors, including the growth, proliferation, clone formation, apoptosis, and migration of human laryngeal carcinoma cells (Hep-2 cells were observed. The influences on apoptotic protease activity factor-1 (APAF-1-mediated apoptosis pathway and the activities of matrix metalloproteinases (MMP-2 and MMP-9 were measured by Western blotting and gelatin zymography assay. Results: Half-maximal inhibitory concentration of fasudil to Hep-2 cells was ~3.40×103 µM (95% CI: 2.53–4.66×103 µM. Moreover, fasudil treatment significantly decreased the ability of growth, proliferation, clone formation, and migration of Hep-2 cells, while remarkably increased the apoptosis rate. Furthermore, the expressions of APAF-1, caspase-9, and caspase-3 significantly increased in fasudil treatment group. Meanwhile, fasudil led to a remarkable decrease in the expressions and activities of MMP-2 and MMP-9. Conclusion: Our findings first demonstrate that fasudil not only inhibits the proliferation of laryngeal carcinoma cells through activating APAF-1-mediated apoptosis pathway, but also prevents migration by inhibiting the activities of MMP-2 and MMP-9. Therefore, fasudil is an attractive antitumor drug candidate for the treatment of laryngeal carcinoma

  3. Pine needle oil induces G2/M arrest of HepG2 cells by activating the ATM pathway.

    Science.gov (United States)

    Qiu, Bing; Jiang, Wei; Qiu, Wenliang; Mu, Wenling; Qin, Yujing; Zhu, Yongcui; Zhang, Jianying; Wang, Qingyi; Liu, Dongjie; Qu, Zhangyi

    2018-02-01

    Over the last two decades, inducing DNA damage of cancer cells by natural medicines has become a research hotspot in the field of cancer treatment. Although various natural medicines have anticancer effects, very few studies have been conducted to explore the anti-cancer effect of pine needle oil. In the present study, the role of pine needle oil in inducing G2/M arrest in HepG2 cells was investigated. The data revealed that pine needle oil could induce DNA damage in a dose-dependent manner. In the pine needle oil-treated HepG2 cells, the protein levels of phosphorylated (p)-ataxia-telangiectasia mutated (ATM), γ-H2A histone family, member X, p-p53, p-checkpoint kinase 2 and p-cell division cycle 25C were evidently increased, indicating that pine needle oil facilitated G2/M arrest in HepG2 cells through the ATM pathway. In response to the treatment with pine needle oil, ATM was activated in HepG2 cells, which subsequently phosphorylated downstream targets and induced G2/M arrest. In summary, the data of the present study indicated that pine needle oil induces G2/M arrest in HepG2 cells by facilitating ATM activation.

  4. [Correlation between histone H3-K9 methylation, DNA methylation and expression of gene MGMT in Hep-2 cell line].

    Science.gov (United States)

    Yang, Jing; He, Liria; Ji, Wenyue; Jin, Mingzhu; Zhao, Xudong

    2012-11-01

    To explore the correlation between histone H3-K9 methylation, DNA methylation and expression of carcinoma suppressor gene MGMT in laryngeal carcinoma Hep-2 cell line. 5-Aza-dC was used to deal with Hep-2 cell cultured in vitro. ChIP, MSP and Realtime-PCR were used to detect H3-K9 methylation, DNA methylation, of MGMT gene promoter region and MGMT gene expression before and after treatment with drugs. (1) In Hep-2 cell line, gene MGMT was characterized by DNA methylation and histone H3-K9 hypermethylation. (2) 5-Aza-dC was able to reduce H3-K9 methylation of MGMT gene histone in Hep-2 cell line, 5-Aza-dC was able to reverse DNA methylation of MGMT gene histone in Hep-2 cell line, 5-Aza-dC was able to upregulate the down-regulated gene expression of tumor suppressor genes MGMT. Promoter methylation of cancer suppressor gene MGMT may induce the gene inactivity. DNA methylation may increase H3-K9 methylation. 5-Aza-dC can reduce H3-K9 methylation of tumor suppressor gene MGMT histone by reversing DNA methylation of tumor suppressor gene MGMT, and then the expression of tumor suppressor genes is increased and tumor development is inhibited.

  5. Mechanism of Arctigenin-Induced Specific Cytotoxicity against Human Hepatocellular Carcinoma Cell Lines: Hep G2 and SMMC7721

    Science.gov (United States)

    Lu, Zheng; Cao, Shengbo; Zhou, Hongbo; Hua, Ling; Zhang, Shishuo; Cao, Jiyue

    2015-01-01

    Arctigenin (ARG) has been previously reported to exert high biological activities including anti-inflammatory, antiviral and anticancer. In this study, the anti-tumor mechanism of ARG towards human hepatocellular carcinoma (HCC) was firstly investigated. We demonstrated that ARG could induce apoptosis in Hep G2 and SMMC7721 cells but not in normal hepatic cells, and its apoptotic effect on Hep G2 was stronger than that on SMMC7721. Furthermore, the following study showed that ARG treatment led to a loss in the mitochondrial out membrane potential, up-regulation of Bax, down-regulation of Bcl-2, a release of cytochrome c, caspase-9 and caspase-3 activation and a cleavage of poly (ADP-ribose) polymerase in both Hep G2 and SMMC7721 cells, suggesting ARG-induced apoptosis was associated with the mitochondria mediated pathway. Moreover, the activation of caspase-8 and the increased expression levels of Fas/FasL and TNF-α revealed that the Fas/FasL-related pathway was also involved in this process. Additionally, ARG induced apoptosis was accompanied by a deactivation of PI3K/p-Akt pathway, an accumulation of p53 protein and an inhibition of NF-κB nuclear translocation especially in Hep G2 cells, which might be the reason that Hep G2 was more sensitive than SMMC7721 cells to ARG treatment. PMID:25933104

  6. Mechanism of Arctigenin-Induced Specific Cytotoxicity against Human Hepatocellular Carcinoma Cell Lines: Hep G2 and SMMC7721.

    Directory of Open Access Journals (Sweden)

    Zheng Lu

    Full Text Available Arctigenin (ARG has been previously reported to exert high biological activities including anti-inflammatory, antiviral and anticancer. In this study, the anti-tumor mechanism of ARG towards human hepatocellular carcinoma (HCC was firstly investigated. We demonstrated that ARG could induce apoptosis in Hep G2 and SMMC7721 cells but not in normal hepatic cells, and its apoptotic effect on Hep G2 was stronger than that on SMMC7721. Furthermore, the following study showed that ARG treatment led to a loss in the mitochondrial out membrane potential, up-regulation of Bax, down-regulation of Bcl-2, a release of cytochrome c, caspase-9 and caspase-3 activation and a cleavage of poly (ADP-ribose polymerase in both Hep G2 and SMMC7721 cells, suggesting ARG-induced apoptosis was associated with the mitochondria mediated pathway. Moreover, the activation of caspase-8 and the increased expression levels of Fas/FasL and TNF-α revealed that the Fas/FasL-related pathway was also involved in this process. Additionally, ARG induced apoptosis was accompanied by a deactivation of PI3K/p-Akt pathway, an accumulation of p53 protein and an inhibition of NF-κB nuclear translocation especially in Hep G2 cells, which might be the reason that Hep G2 was more sensitive than SMMC7721 cells to ARG treatment.

  7. Borax-induced apoptosis in HepG2 cells involves p53, Bcl-2, and Bax.

    Science.gov (United States)

    Wei, Y; Yuan, F J; Zhou, W B; Wu, L; Chen, L; Wang, J J; Zhang, Y S

    2016-06-21

    Borax, a boron compound and a salt of boric acid, is known to inhibit the growth of tumor cells. HepG2 cells have been shown to be clearly susceptible to the anti-proliferative effects of borax. However, the specific mechanisms regulating this effect are poorly understood. This study aimed to investigate the pathways underlying the growth inhibition induced by borax in HepG2 cells. The effects of borax on HepG2 cell viability were characterized using MTT. Apoptosis was also verified by annexin V/propidium iodide staining. JC-1 dye and western blotting techniques were used to measure mitochondrial membrane potential and p53, Bax, and Bcl-2 protein expression, respectively. Relevant mRNA levels were measured by qRT-PCR. Borax inhibited the proliferation of HepG2 cells in a time- and dose-dependent manner in vitro. The apoptotic process triggered by borax involved the upregulation of p53 and Bax and the downregulation of Bcl-2, which was confirmed by a change in the mitochondrial membrane potential. These results elucidate a borax-induced apoptotic pathway in HepG2 cells that involves the upregulation of p53 and Bax and the downregulation of Bcl-2.

  8. Casearin D inhibits ERK phosphorylation and induces downregulation of cyclin D1 in HepG2 cells.

    Science.gov (United States)

    Ferreira-Silva, Guilherme Álvaro; Lages, Carla Carolina Lopes; Sartorelli, Patricia; Hasegawa, Flávia Rie; Soares, Marisi Gomes; Ionta, Marisa

    2017-02-01

    Cancer is a public health problem which represents the second cause of death in the world. In this framework, it is necessary to identify novel compounds with antineoplastic potential. Plants are an important source for discovering novel compounds with pharmacological potential. In this study, we aimed to investigate the antiproliferative potential of isolated compounds from Casearia sylvestris on tumor cell lines. Crude extract effectively reduced cell viability of 4 tumor cell lines (HepG2, A549, U251-MG, and HT-144) after 48h treatment. HepG2 and HT-144 were the most responsive cells. Three fractions (aqueous ethanol, n-hexane and ethyl acetate) were tested against HepG2 and HT-144 cells and we observed that compounds with antiproliferative activity were concentrated in n-hexane and ethyl acetate fractions. The casearins A, G and J were isolated from n-hexane fraction, while casearin D was obtained from ethyl acetate fraction. We demonstrated that casearin D significantly inhibited the clonogenic capacity of HepG2 cells after 24h exposure indicating its antiproliferative activity. In addition, G1/S transition cell cycle arrest in HepG2 cells was also observed. These effects are related, at least in part, to ability of the casearin D in reducing ERK phosphorylation and cyclin D1 expression levels. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. Extracellular visfatin activates gluconeogenesis in HepG2 cells through the classical PKA/CREB-dependent pathway.

    Science.gov (United States)

    Choi, Y J; Choi, S-E; Ha, E S; Kang, Y; Han, S J; Kim, D J; Lee, K W; Kim, H J

    2014-04-01

    Adipokines reportedly affect hepatic gluconeogenesis, and the adipokine visfatin is known to be related to insulin resistance and type 2 diabetes. However, whether visfatin contributes to hepatic gluconeogenesis remains unclear. Visfatin, also known as nicotinamide phosphoribosyltransferase (NAMPT), modulates sirtuin1 (SIRT1) through the regulation of nicotinamide adenine dinucleotide (NAD). Therefore, we investigated the effect of extracellular visfatin on glucose production in HepG2 cells, and evaluated whether extracellular visfatin affects hepatic gluconeogenesis via an NAD+-SIRT1-dependent pathway. Treatment with visfatin significantly increased glucose production and the mRNA expression and protein levels of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) in HepG2 cells in a time- and concentration-dependent manner. Knockdown of SIRT1 had no remarkable effect on the induction of gluconeogenesis by visfatin. Subsequently, we evaluated if extracellular visfatin stimulates the production of gluconeogenic enzymes through the classical protein kinase A (PKA)/cyclic AMP-responsive element (CRE)-binding protein (CREB)-dependent process. The phosphorylation of CREB and PKA increased significantly in HepG2 cells treated with visfatin. Additionally, knockdown of CREB and PKA inhibited visfatin-induced gluconeogenesis in HepG2 cells. In summary, extracellular visfatin modulates glucose production in HepG2 cells through the PKA/CREB pathway, rather than via SIRT1 signaling. © Georg Thieme Verlag KG Stuttgart · New York.

  10. Regulation of human CYP27A1 by estrogens and androgens in HepG2 and prostate cells.

    Science.gov (United States)

    Tang, Wanjin; Norlin, Maria; Wikvall, Kjell

    2007-06-01

    The regulation of the human CYP27A1 gene by estrogens and androgens was studied in human liver-derived HepG2 and prostate cells. Our results show that the promoter activity, enzymatic activity and mRNA levels of CYP27A1 in HepG2 cells are downregulated by estrogen in presence of ERalpha or ERbeta. Similar effects by estrogen were found in RWPE-1 prostate cells. In contrast, estrogen markedly upregulated the transcriptional activity of CYP27A1 in LNCaP prostate cancer cells. 5alpha-Dihydrotestosterone and androgen receptor upregulated the transcriptional activity of CYP27A1 in HepG2 cells. Progressive deletion experiments indicate that the ERbeta-mediated effects in HepG2 and LNCaP cells are conferred to the same region (-451/+42) whereas ERalpha-mediated effects on this promoter are more complex. The results indicate that the stimulating effect of androgen in HepG2 cells is conferred to a region upstream from -792 in the CYP27A1 promoter. In summary, we have identified the human CYP27A1 gene as a target for estrogens and androgens. The results imply that expression of CYP27A1 may be affected by endogenous sex hormones and pharmacological compounds with estrogenic or androgenic effects.

  11. Efecto Protector de Peumus Boldus en ratas con toxicidad hepática inducida por Paracetamol

    Directory of Open Access Journals (Sweden)

    Christiam Ochoa

    2008-01-01

    Full Text Available Objetivo: Comprobar el efecto protector hepático del extracto acuoso de boldo (EAB (Peumus boldus al daño hepático inducido por acetaminofén (paracetamol. Diseño: Analítico, experimental, aleatorizado, completo ¿ experimento verdadero. Realizado en el Instituto de Patología de la Facultad de Medicina de UNMSM. Material y método. 30 Ratas Holtzman macho de 250g y dos meses se dividieron en 5 grupos aleatoriamente, grupo blanco, control paracetamol 200mg/kg y 3 experimentales tratados con EAB a 80 mg/kg, 120 mg/kg y 160 mg/kg respectivamente. Se administró EAB de 80 mg/kg, 120 mg/kg y 160 mg/kg vía orogástrica. Luego de media hora se administró paracetamol 200mg/kg i.p. a los grupos control y experimentales. Este procedimiento se repitió por 5 días. Se tomó pruebas de transaminasas (TGP basal y final en sangre. Se utilizó la prueba de Kruskal-Wallis para analizar la data y un p<0.05 fue considerado significante. Se estudió la anatomopatología de los hígados y se tomaron muestras de tejido teñidas con HE. Resultados: Existe diferencia significativa en los niveles de transaminasas (TGP entre grupos (p<0.05. El control obtuvo 196.6 U/L +- 38.1 (TGP mientras que los experimentales como máximo 55.6 U/l. Las muestras control evidencian signos de lesión hepática, degeneración grasa, congestión sinusoidal y centrolobulillar, y necrosis celular. Sin embargo los grupos experimentales no presentan signos de lesión celular y hay ausencia de inflamación. Conclusiones: El boldo tiene un efecto protector hepático al daño inducido por paracetamol en ratas Holtzmann.

  12. Bridging FORTRAN to object oriented paradigm for HEP data modeling task

    International Nuclear Information System (INIS)

    Huang, J.

    1993-12-01

    Object oriented (OO) technology appears to offer tangible benefits to the high energy physics (HEP) community. Yet many physicists view this newest software development used with much reservation and reluctance. Facing the reality of having to support the existing physics applications, which are written in FORTRAN, the software engineers in the Computer Engineering Group of the Physics Research Division at the Superconducting Super Collider Laboratory have accepted the challenge of mixing an old language with the new technology. This paper describes the experience and the techniques devised to fit FORTRAN into the OO paradigm (OOP)

  13. Parásitos de Fasciola hepática intracoledociano

    OpenAIRE

    MILLÁN A,MARCELO; WAGENKNECHT S,RAUL; CARDENAS P,ALEX; CARRASCO L,CRISTIAN

    2008-01-01

    La fasciolasis es una enfermedad parasitaria producida por el tremátodo digeneo Fasciola hepática. Durante la fase crónica de la enfermedad los parásitos pueden entran en los conductos biliares, y ser causa de ictericia obstructiva, colangitis, pancreatitis y otras complicaciones. Se presenta el caso clínico de una paciente intervenida por sospecha de colelitiasis y colédocolitiasis, a la cual durante la exploración coledociana intraoperatoria le fueron extraídos múltiples parásitos adultos d...

  14. HEPS4Power - Extended-range Hydrometeorological Ensemble Predictions for Improved Hydropower Operations and Revenues

    Science.gov (United States)

    Bogner, Konrad; Monhart, Samuel; Liniger, Mark; Spririg, Christoph; Jordan, Fred; Zappa, Massimiliano

    2015-04-01

    In recent years large progresses have been achieved in the operational prediction of floods and hydrological drought with up to ten days lead time. Both the public and the private sectors are currently using probabilistic runoff forecast in order to monitoring water resources and take actions when critical conditions are to be expected. The use of extended-range predictions with lead times exceeding 10 days is not yet established. The hydropower sector in particular might have large benefits from using hydro meteorological forecasts for the next 15 to 60 days in order to optimize the operations and the revenues from their watersheds, dams, captions, turbines and pumps. The new Swiss Competence Centers in Energy Research (SCCER) targets at boosting research related to energy issues in Switzerland. The objective of HEPS4POWER is to demonstrate that operational extended-range hydro meteorological forecasts have the potential to become very valuable tools for fine tuning the production of energy from hydropower systems. The project team covers a specific system-oriented value chain starting from the collection and forecast of meteorological data (MeteoSwiss), leading to the operational application of state-of-the-art hydrological models (WSL) and terminating with the experience in data presentation and power production forecasts for end-users (e-dric.ch). The first task of the HEPS4POWER will be the downscaling and post-processing of ensemble extended-range meteorological forecasts (EPS). The goal is to provide well-tailored forecasts of probabilistic nature that should be reliable in statistical and localized at catchment or even station level. The hydrology related task will consist in feeding the post-processed meteorological forecasts into a HEPS using a multi-model approach by implementing models with different complexity. Also in the case of the hydrological ensemble predictions, post-processing techniques need to be tested in order to improve the quality of the

  15. Análisis de supervivencia de pacientes obesos sometidos a trasplante hepático

    OpenAIRE

    Torres-Quevedo Quevedo, Rodrigo

    2015-01-01

    La prevalencia de obesidad ha aumentado en países en vías de desarrollo, de igual modo en receptores de trasplantes hepático (TH) ésta también se ha incrementado de manera significativa. La obesidad tiene un impacto negativo, en cirugía general y trasplante de órgano sólido. Hasta la fecha existen pocos estudios en TH que analizan el impacto de la obesidad en los resultados del mismo. Objetivo: determinar si existen diferencias en la supervivencia (SV) actuarial a 5 años de obesos y no obe...

  16. Cirrosis hepática: medidas preventivas de algunas de sus complicaciones

    OpenAIRE

    A. Claudio L. Toledo, Dr.

    2010-01-01

    Cirrosis hepática es una enfermedad prevalente en el mundo. Su diagnóstico y tratamiento debe ser dominio de todo médico general e internista. En los últimos años han ocurrido notables avances en el diagnóstico, fisiopatología, tratamiento y en la identificación de factores pronósticos de cirrosis y de sus complicaciones mayores. Particular atención ha existido en desarrollar medidas preventivas de sus complicaciones como por ejemplo: hemorragia por várices esofágicas, peritonitis bacte...

  17. Absceso hepático asociado a absceso pulmonar y endoftalmitis

    OpenAIRE

    Jairo Cordero-Chen; Eduardo Catalán-Sánchez; Juan Ignacio Padilla-Cuadra; Jorge Ramírez-Arce

    2013-01-01

    El absceso hepático piógeno producido por Klebsiella pneumoniae es relativamente raro y puede complicarse con lesiones sépticas a distancia. Esto se relaciona con características propias del germen que incluyen el genotipo K1, resistencia a la fagocitosis y la presencia del gen mag-A. Tales metástasis sépticas contemplan absceso pulmonar, meningitis, endocarditis bacteriana y, muy especialmente, endoftalmitis. Esta última ocurre con mayor frecuencia en pacientes diabéticos, y puede causar ceg...

  18. Toxicidad hepática por medicamentos antituberculosos Hepatotoxicity induced by antituberculosis drugs

    Directory of Open Access Journals (Sweden)

    Isabel Eugenia Escobar Toledo

    2008-01-01

    Full Text Available

    El fenómeno de la toxicidad hepática inducida por medicamentos cobró relevancia hace algunos años con el estudio de las reacciones adversas a medicamentos. El daño producido en el hígado por un xenobiótico que altera su función es lo que se conoce como toxicidad hepática. La importancia de reconocer y diagnosticar la toxicidad hepática por medicamentos estriba en su gravedad potencial; no en vano es la causa más frecuente por la que la industria farmacéutica retira medicamentos. La tuberculosis es una pandemia que afecta a gran parte de la población mundial y junto con el VIH es una enfermedad cada vez más frecuente en Colombia. Esta enfermedad se puede considerar como una situación especial porque para su tratamiento es preciso suministrar, por largos períodos, medicamentos con potencial tóxico para el hígado.

     

    El objetivo de este artículo es revisar algunos aspectos relacionados con la toxicidad hepática secundaria a medicamentos antituberculosos, tales como: epidemiología, factores de riesgo, mecanismos de toxicidad, manifestaciones clínicas, diagnóstico, tratamiento y seguimiento.

    Hepatotoxicity is the alteration of liver structure and function induced by either drugs or other substances. The importance of its proper diagnosis rests on its potential severity. It is the most frequent reason by which the pharmaceutical industry withdraws its products. Tuberculosis is a pandemic infection affecting a large proportion of the world population. Together with HIV infection it is becoming ever more frequent in Colombia. Tuberculosis poses

  19. Terapia nutricional no transplante hepático Nutritional therapy in liver transplantation

    Directory of Open Access Journals (Sweden)

    Mônica Beatriz PAROLIN

    2002-04-01

    Full Text Available Racional - Deficiências nutricionais, por vezes graves, são comuns em pacientes com insuficiência hepática, candidatos a transplante de fígado. A terapia nutricional pode corrigir total ou parcialmente tais deficiências, melhorando as condições clínicas e o prognóstico desses indivíduos, frente ao grande desafio do transplante hepático. Objetivos - Breve revisão do papel do fígado no metabolismo dos diversos nutrientes. Descrição dos métodos de avaliação do estado nutricional, traçando-se as bases da terapia nutricional segundo condições hepáticas diversas, no pré e pós-transplante, em relação às necessidades calóricas e dos diversos nutrientes. Apresentação de intervenções nutricionais, no controle das complicações metabólicas resultantes do uso de drogas imunossupressoras. Conclusão - A terapia nutricional é valiosa aliada no tratamento clínico de pacientes candidatos ou já submetidos ao transplante hepático, contribuindo para um prognóstico favorável e para a melhora da qualidade de vida desses indivíduos.Background - Malnutrition, sometimes severe is common in patients with chronic hepatic diseases who are candidates for liver transplantation. Nutritional therapy can induce partial or total correction of such deficiencies, improving clinical conditions and prognosis of patients who face the great defiance of liver transplantation. Aims - Brief revision of hepatic role in the metabolism of several nutrients. Description of available methods of dietary therapy and its application both under different abnormal hepatic conditions and pre and post-transplant periods. The role of nutritional intervention in metabolic side effects due to immunosuppressive drugs. Conclusion - Nutritional therapy is a valuable adjuvant resource to the clinical treatment of candidates and submitted patients to hepatic transplantation providing better prognosis and improved life quality.

  20. Measurement of Hepatic Protein Fractional Synthetic Rate with Stable Isotope Labeling Technique in Thapsigargin Stressed HepG2 Cells

    Science.gov (United States)

    Song, Juquan; Zhang, Xiao-jun; Boehning, Darren; Brooks, Natasha C.; Herndon, David N.; Jeschke, Marc G.

    2012-01-01

    Severe burn-induced liver damage and dysfunction is associated with endoplasmic reticulum (ER) stress. ER stress has been shown to regulate global protein synthesis. In the current study, we induced ER stress in vitro and estimated the effect of ER stress on hepatic protein synthesis. The aim was two-fold: (1) to establish an in vitro model to isotopically measure hepatic protein synthesis and (2) to evaluate protein fractional synthetic rate (FSR) in response to ER stress. Human hepatocellular carcinoma cells (HepG2) were cultured in medium supplemented with stable isotopes 1,2-13C2-glycine and L-[ring-13C6]phenylalanine. ER stress was induced by exposing the cells to 100 nM of thapsigargin (TG). Cell content was collected from day 0 to 14. Alterations in cytosolic calcium were measured by calcium imaging and ER stress markers were confirmed by Western blotting. The precursor and product enrichments were detected by GC-MS analysis for FSR calculation. We found that the hepatic protein FSR were 0.97±0.02 and 0.99±0.05%/hr calculated from 1,2-13C2-glycine and L-[ring-13C6]phenylalanine, respectively. TG depleted ER calcium stores and induced ER stress by upregulating p-IRE-1 and Bip. FSR dramatically decreased to 0.68±0.03 and 0.60±0.06%/hr in the TG treatment group (pisotope tracer incorporation technique is a useful method for studying the effects of ER stress on hepatic protein synthesis. PMID:22298954

  1. Schistosomiasis: predisposing cause for the formation of hepatic abscesses? Case report Esquistossomose: causa predisponente para a formação de abscessos hepáticos?

    Directory of Open Access Journals (Sweden)

    Vasco Carvalho Pedroso de Lima

    1995-06-01

    Full Text Available An adult patient with chronic schistosomiasis from an endemic area, complained about a seven day fever, along with jaundice and lumbar backache on the right side. Image exams showed multiple pyogenic liver abscesses. All the classic etiologies were discarded through clinical, radiological and laboratorial criteria. Schistosomiasis can cause pylephlebitis as a complication, along with immunesuppression, granulomatous reaction with central lobular liver necrosis and a greater risk of infection. The authors suggest that schistosomiasis in its chronic form may be the predisposing cause of multiple pyogenic liver abscesses, especially in endemic areas.Paciente adulto, natural de região endêmica para esquistossomose e portador crônico da doença, apresentava queixa de febre há sete dias, associada à ictericia e dor lombar em região direita. Os exames radiológicos mostraram abscessos hepáticos piogênicos múltiplos, cuja causa predisponente é conhecida, segundo trabalhos da literatura, em 100% dos casos. Através de parâmetros clínicos, laboratoriais e radiológicos todas as etiologias clássicas foram afastadas. Sabe-se que a esquistossomose pode provocar, como complicação, a pileflebite, além de depressão imunológica e reação granulomatosa com necrose lobular central e maior risco de infecção. Os autores deste relato de caso sugerem ser a esquistossomose, na sua forma crônica, causa predisponente para formação de abscessos hepáticos piogênicos múltiplos, principalmente em regiões endêmicas.

  2. Efeito da ressecção do íleo terminal na fibrose hepática secundária à ligadura do ducto hepático comum em ratas

    Directory of Open Access Journals (Sweden)

    Evandro Luis de Oliveira Costa

    Full Text Available OBJETIVO: A evolução para fibrose hepática e, posteriormente, para cirrose são fatos bem estabelecidos na colestase extra-hepática prolongada. A despeito dos avanços nos métodos diagnósticos e terapêuticos, essas complicações continuam de difícil solução, principalmente, quando não é possível reverter a causa da colestase. Neste trabalho, procurou-se verificar, em modelo experimental de colestase pela ligadura do ducto hepático comum, se a exclusão do íleo terminal reduziria o desenvolvimento de fibrose hepática. Não houve abordagem direta da causa da colestase, mas atuou-se nos mecanismos de secreção e regulação do fluxo biliar êntero-hepático. MÉTODO: Foram utilizadas trinta e cinco ratas Wistar, distribuídas em três grupos: Grupo 1, apenas laparotomia e laparorrafia; Grupo 2, ligadura do ducto hepático comum; Grupo 3, ligadura do ducto hepático comum associada a ressecção do íleo terminal, com reconstrução do trânsito intestinal, por meio de anastomose íleo-cólon ascendente. Após trinta dias, os animais foram mortos e o fígado de cada rata foi retirado, para a análise histológica. RESULTADOS: Os resultados foram submetidos a análise estatística pelo teste de Kuskal-Wallis, com nível de significância de 95 % (p < 0,05. Verificou-se que houve fibrose hepática nos grupos 2 e 3, porém sem cirrose. O Grupo 3 apresentou fibrose menos acentuada que o Grupo 2. CONCLUSÕES: Conclui-se que a ressecção do íleo terminal associa-se a menor alteração histológica, no fígado de ratas, decorrente de colestase obstrutiva.

  3. Coccidiosis hepática en el conejo: aspectos ambientales y clínico-patológicos

    Directory of Open Access Journals (Sweden)

    Mario Pérez Martínez

    2011-01-01

    Full Text Available La cunicultura es una industria creciente en México. De las 13 especies de coccidias que afectan a los conejos, la Eimeria stiedae es el principal agente causal de la coccidiosis hepática en el conejo. En la presente revisión se aborda la importancia del medio ambiente y aspectos clínico- patológicos de la coccidiosis hepática en conejos. Como resultado de la revisión de la literatura disponible se observa la necesidad de abordar el estudio de la coccidiosis hepática del conejo de manera integral, y avanzar en la comprensión de los mecanismos celulares y moleculares involucrados en su patogenia.

  4. Inflammation response at the transcriptional level of HepG2 cells induced by multi-walled carbon nanotubes

    Energy Technology Data Exchange (ETDEWEB)

    Piret, Jean-Pascal; Vankoningsloo, Sebastien; Noel, Florence; Saout, Christelle; Toussaint, Olivier [Research Unit in Cellular Biology (URBC), Narilis, University of Namur, 5000 Namur (Belgium); Mendoza, Jorge Mejia; Lucas, Stephane, E-mail: olivier.toussaint@fundp.ac.be [Research Center for the Physics of Matter and Radiation (PMR), Narilis, University of Namur, 5000 Namur (Belgium)

    2011-07-06

    Poor information are currently available about the biological effects of multi-walled carbon nanotubes (MWCNT) on the liver. In this study, we evaluated the effects of MWCNT at the transcriptional level on the classical in vitro model of HepG2 hepatocarcinoma cells. The expression levels of 96 transcript species implicated in the inflammatory and immune responses was studied after a 24h incubation of HepG2 cells in presence of raw MWCNT dispersed in water by stirring. Among the 46 transcript species detected, only a few transcripts including mRNA coding for interleukine-7, chemokines receptor of the C-C families CCR7, as well as Endothelin-1, were statistically more abundant after treatment with MWCNT. Altogether, these data indicate that MWCNT can only induce a weak inflammatory response in HepG2 cells.

  5. Inflammation response at the transcriptional level of HepG2 cells induced by multi-walled carbon nanotubes

    Science.gov (United States)

    Piret, Jean-Pascal; Vankoningsloo, Sébastien; Noël, Florence; Mejia Mendoza, Jorge; Lucas, Stéphane; Saout, Christelle; Toussaint, Olivier

    2011-07-01

    Poor information are currently available about the biological effects of multi-walled carbon nanotubes (MWCNT) on the liver. In this study, we evaluated the effects of MWCNT at the transcriptional level on the classical in vitro model of HepG2 hepatocarcinoma cells. The expression levels of 96 transcript species implicated in the inflammatory and immune responses was studied after a 24h incubation of HepG2 cells in presence of raw MWCNT dispersed in water by stirring. Among the 46 transcript species detected, only a few transcripts including mRNA coding for interleukine-7, chemokines receptor of the C-C families CCR7, as well as Endothelin-1, were statistically more abundant after treatment with MWCNT. Altogether, these data indicate that MWCNT can only induce a weak inflammatory response in HepG2 cells.

  6. Efecto del sildenafilo sobre la neuroinflamacion en ratas con encefalopatía hepática

    OpenAIRE

    IBÁÑEZ GRAU, ANDREA

    2016-01-01

    [ES] La Encefalopatía Hepática (EH) es un síndrome neuropsiquiátrico complejo producido como consecuencia de un fallo hepático agudo o crónico. La EH tiene 4 fases, empieza con alteraciones de la personalidad y atención, progresa con una disminución de la función cognitiva y motora y finalmente provoca coma y muerte. Este fallo hepático provoca una disminución de la función del hígado, y por tanto, de la correcta detoxificación, acumulándose diferentes sustancias en el organismo entre las que...

  7. Isquemia hepÃtica experimental e a aÃÃo da l-alanil-glutamina

    OpenAIRE

    Raimundo Jose Cunha AraÃjo Junior

    2011-01-01

    As estratÃgias para prevenir a lesÃo de isquemia reperfusÃo durante as cirurgias hepÃticas incluem a oclusÃo intermitente do fluxo de sangue ao fÃgado ou a utilizaÃÃo de substÃncias que poderiam causar um efeito protetor ou aumento da resistÃncia do tecido hepÃtico à isquemia. O presente estudo teve o objetivo de avaliar o efeito do uso da L-Alanil-Glutamina (GLN) em Ratus novergicus submetidos à isquemia hepÃtica normotÃrmica, atravÃs de provas bioquÃmicas e imunohistoquÃmicas. Utilizou-se...

  8. Representações sociais sobre cirrose hepática alcoólica elaboradas por seus portadores

    OpenAIRE

    Rocha,Edilma Gomes; Pereira,Maria Lúcia Duarte

    2007-01-01

    O abuso do álcool pode levar à cirrose hepática alcoólica, problema de saúde pública e de alto custo social. Objetivou-se apreender as representações sobre cirrose hepática alcoólica elaboradas por seus portadores. Metodologia: estudo descritivo, exploratório, com abordagem qualitativa desenvolvido em duas instituições públicas no município de Fortaleza-CE. Participaram quinze sujeitos com diagnóstico de cirrose hepática alcoólica. Aplicou-se entrevista semi-estruturada, e foi utilizada técni...

  9. Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2.

    Science.gov (United States)

    Schwarzlin, Romina; Pušenjak, Nika; Makuc, Damjan; Križman, Mitja; Vovk, Irena; Plavec, Janez; Švajger, Urban

    2016-10-18

    It had been demonstrated that sugars from various plants can act as potent agents, which induce apoptosis of cancer cells. Using HPLC, we fractionated a mixture of two plant extracts from the plant family Solanaceae, namely Capsicum chinense and the plant family Amaryllidaceae namely Allium sativum. We evaluated the effect of different fractions on apoptosis of HepG2 cell line. The most effective fraction was further studied to determine its molecular composition using mass spectrometry (MS) and NMR. We further evaluated the effect of determined molecular composition found in the selected fraction by using a mixture of commercially available substances, which were found in the fraction and tested its pro-apoptotic effect on HepG2 cells. To get some insight into potential apoptotic mechanisms we studied caspase-3 activity and mitochondrial integrity in treated cells. Out of 93 fractions obtained by HPLC from the plant extract we found HPLC fraction 10 (10 min elution) was the most effective. MS and NMR studies revealed high presence of cellobiose together with vitamin C, sulphur (S) and trace amounts of selenium (Se). HPLC fraction 10 triggered apoptosis of HepG2 within 3 h in the 0.01-1.0 mg/mL concentration range. Furthermore, a mixture of pure cellobiose, vitamin C, S and Se (complex cellobiose/C/S/Se) had a very similar capacity in inducing apoptosis of HepG2 cells compared to HPLC fraction 10. Complex cellobiose/C/S/Se was capable of inducing caspase-3 activity and led to loss of mitochondrial integrity. The capacity of cellobiose alone to induce apoptosis of HepG2 was approximately 1000-fold lower compared to complex cellobiose/C/S/Se. In this study we present the highly synergistic effect of a unique complex consisting of cellobiose, vitamin C, sulphur and selenium on triggering the apoptosis of human hepatocellular carcinoma (HepG2) cell line.

  10. Crambescin C1 Exerts a Cytoprotective Effect on HepG2 Cells through Metallothionein Induction

    Science.gov (United States)

    Roel, María; Rubiolo, Juan A.; Ternon, Eva; Thomas, Olivier P.; Vieytes, Mercedes R.; Botana, Luis M.

    2015-01-01

    The Mediterranean marine sponge Crambe crambe is the source of two families of guanidine alkaloids known as crambescins and crambescidins. Some of the biological effects of crambescidins have been previously reported while crambescins have undergone little study. Taking this into account, we performed comparative transcriptome analysis to examine the effect of crambescin-C1 (CC1) on human tumor hepatocarcinoma cells HepG2 followed by validation experiments to confirm its predicted biological activities. We report herein that, while crambescin-A1 has a minor effect on these cells, CC1 protects them against oxidative injury by means of metallothionein induction even at low concentrations. Additionally, at high doses, CC1 arrests the HepG2 cell cycle in G0/G1 and thus inhibits tumor cell proliferation. The findings presented here provide the first detailed approach regarding the different effects of crambescins on tumor cells and provide a basis for future studies on other possible cellular mechanisms related to these bioactivities. PMID:26225985

  11. An overview of the DII-HEP OpenStack based CMS data analysis

    Science.gov (United States)

    Osmani, L.; Tarkoma, S.; Eerola, P.; Komu, M.; Kortelainen, M. J.; Kraemer, O.; Lindén, T.; Toor, S.; White, J.

    2015-05-01

    An OpenStack based private cloud with the Cluster File System has been built and used with both CMS analysis and Monte Carlo simulation jobs in the Datacenter Indirection Infrastructure for Secure High Energy Physics (DII-HEP) project. On the cloud we run the ARC middleware that allows running CMS applications without changes on the job submission side. Our test results indicate that the adopted approach provides a scalable and resilient solution for managing resources without compromising on performance and high availability. To manage the virtual machines (VM) dynamically in an elastic fasion, we are testing the EMI authorization service (Argus) and the Execution Environment Service (Argus-EES). An OpenStackplugin has been developed for Argus-EES. The Host Identity Protocol (HIP) has been designed for mobile networks and it provides a secure method for IP multihoming. HIP separates the end-point identifier and locator role for IP address which increases the network availability for the applications. Our solution leverages HIP for traffic management. This presentation gives an update on the status of the work and our lessons learned in creating an OpenStackbased cloud for HEP.

  12. Red River Wildlife Management Area HEP Report, Habitat Evaluation Procedures, Technical Report 2004.

    Energy Technology Data Exchange (ETDEWEB)

    Ashley, Paul

    2004-11-01

    A habitat evaluation procedures (HEP) analysis conducted on the 314-acre Red River Wildlife Management Area (RRWMA) managed by the Idaho Department of Fish and Game resulted in 401.38 habitat units (HUs). Habitat variables from six habitat suitability index (HSI) models, comprised of mink (Mustela vison), mallard (Anas platyrhynchos), common snipe (Capella gallinago), black-capped chickadee (Parus altricapillus), yellow warbler (Dendroica petechia), and white-tailed deer (Odocoileus virginianus), were measured by Regional HEP Team (RHT) members in August 2004. Cover types included wet meadow, riverine, riparian shrub, conifer forest, conifer forest wetland, and urban. HSI model outputs indicate that the shrub component is lacking in riparian shrub and conifer forest cover types and that snag density should be increased in conifer stands. The quality of wet meadow habitat, comprised primarily of introduced grass species and sedges, could be improved through development of ephemeral open water ponds and increasing the amount of persistent wetland herbaceous vegetation e.g. cattails (Typha spp.) and bulrushes (Scirpus spp.).

  13. Effects of perfluorooctanoate and perfluorooctane sulfonate exposure on hepatoma Hep G2 cells

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Xiao-Zhong; Hu, De-Cong [Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan (China)

    2009-09-15

    Perfluorinated compounds (PFCs) are emerging compounds of concern. They are widely distributed in the environment, wildlife and human. Concern has been raised over their possible adverse effects on human health. This study was designed to determine cytotoxic effects of two important PFCs, perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS), in a single and a mixture of them exposure to Hep G2 cells. The results showed that PFOA and PFOS (50-200 {mu}mol/l) induced production of reactive oxygen species (ROS), dissipation of mitochondria membrane potential and apoptosis of Hep G2 cells. Moreover, activities of superoxide dismutase, catalase and glutathione reductase were increased, whereas activities of glutathione-S-transferase and glutathione peroxidase were decreased. Glutathione content was reduced. Differential expression of genes, such as p53, Bcl-2, caspase-9, was evident in PFOA or PFOS exposure groups. The possible mechanism was that they could overwhelm homeostasis of antioxidative systems, boost ROS generation, impact mitochondria, and affect genes expression of apoptotic regulators, which resulted in start-ups of apoptosis program. Cells exposed to mixture of PFOA and PFOS and each of them showed non-apoptotic rate significant difference, which indicated that the combined effect of two compounds was summation effect, but neither synergistic nor antagonistic effect. (orig.)

  14. Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells

    Directory of Open Access Journals (Sweden)

    Jörn Voigt

    2012-01-01

    Full Text Available Indirect immunofluorescence (IIF on human epithelial (HEp-2 cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA. However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF incubation in the market, the automation of result evaluation is still in its infancy. Therefore, the EUROPattern Suite has been developed as a comprehensive automated processing and interpretation system for standardized and efficient ANA detection by HEp-2 cell-based IIF. In this study, the automated pattern recognition was compared to conventional visual interpretation in a total of 351 sera. In the discrimination of positive from negative samples, concordant results between visual and automated evaluation were obtained for 349 sera (99.4%, kappa = 0.984. The system missed out none of the 272 antibody-positive samples and identified 77 out of 79 visually negative samples (analytical sensitivity/specificity: 100%/97.5%. Moreover, 94.0% of all main antibody patterns were recognized correctly by the software. Owing to its performance characteristics, EUROPattern enables fast, objective, and economic IIF ANA analysis and has the potential to reduce intra- and interlaboratory variability.

  15. Automated indirect immunofluorescence evaluation of antinuclear autoantibodies on HEp-2 cells.

    Science.gov (United States)

    Voigt, Jörn; Krause, Christopher; Rohwäder, Edda; Saschenbrecker, Sandra; Hahn, Melanie; Danckwardt, Maick; Feirer, Christian; Ens, Konstantin; Fechner, Kai; Barth, Erhardt; Martinetz, Thomas; Stöcker, Winfried

    2012-01-01

    Indirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF incubation in the market, the automation of result evaluation is still in its infancy. Therefore, the EUROPattern Suite has been developed as a comprehensive automated processing and interpretation system for standardized and efficient ANA detection by HEp-2 cell-based IIF. In this study, the automated pattern recognition was compared to conventional visual interpretation in a total of 351 sera. In the discrimination of positive from negative samples, concordant results between visual and automated evaluation were obtained for 349 sera (99.4%, kappa = 0.984). The system missed out none of the 272 antibody-positive samples and identified 77 out of 79 visually negative samples (analytical sensitivity/specificity: 100%/97.5%). Moreover, 94.0% of all main antibody patterns were recognized correctly by the software. Owing to its performance characteristics, EUROPattern enables fast, objective, and economic IIF ANA analysis and has the potential to reduce intra- and interlaboratory variability.

  16. Antiviral Activity of Sulfated Polysaccharide of Adenanthera pavonina against Poliovirus in HEp-2 Cells

    Directory of Open Access Journals (Sweden)

    Ananda Marques de Godoi

    2014-01-01

    Full Text Available Adenanthera pavonina, popularly known as red-bead tree, carolina, pigeon’s eye, and dragon’s eye, is a plant traditionally used in Brazil for the treatment of several diseases. The present study aimed at evaluating the activity of sulfated polysaccharide from the Adenanthera pavonina (SPLSAp seeds against poliovirus type 1 (PV-1 in HEp-2 cell cultures. The SPLSAp presented a cytotoxic concentration (CC50 of 500 μg/mL in HEp-2 cell cultures, evaluated by the dimethylthiazolyl-diphenyltetrazolium bromide method (MTT. The SPLSAp exhibited a significant antiviral activity, with a 50% inhibitory concentration (IC50 of 1.18 µg/mL, determined by plaque reduction assay and a high selectivity index (SI of 423. The maximum inhibition (100% of PV replication was found when the SPLSAp treatment was concomitant with viral infection (time 0 h, at all tested concentrations. The maximal inhibition was also found when the SPLSAp was used 1 h and 2 h postinfection, albeit at 50 μg/mL and 100 μg/mL. Therefore, we demonstrated that the SPLSAp inhibited PV growth. We also suggested that SPLSAp inhibited PV in more than one step of the replication, as the mechanism of antiviral action. We, therefore, selected the compound as a potential candidate for further development towards the control of the infection.

  17. Proanthocyanidins modulate microRNA expression in human HepG2 cells.

    Directory of Open Access Journals (Sweden)

    Anna Arola-Arnal

    Full Text Available Mi(croRNAs are small non-coding RNAs of 18-25 nucleotides in length that modulate gene expression at the post-transcriptional level. These RNAs have been shown to be involved in a several biological processes, human diseases and metabolic disorders. Proanthocyanidins, which are the most abundant polyphenol class in the human diet, have positive health effects on a variety of metabolic disorders such as inflammation, obesity, diabetes and insulin resistance. The present study aimed to evaluate whether proanthocyanidin-rich natural extracts modulate miRNA expression. Using microarray analysis and Q-PCR, we investigated miRNA expression in HepG2 cells treated with proanthocyanidins. Our results showed that when HepG2 cells were treated with grape seed proanthocyanidin extract (GSPE, cocoa proanthocyanidin extract (CPE or pure epigallocatechin gallate isolated from green tea (EGCG, fifteen, six and five differentially expressed miRNAs, respectively, were identified out of 904 mRNAs. Specifically, miR-30b* was downregulated by the three treatments, and treatment with GSPE or CPE upregulated miR-1224-3p, miR-197 and miR-532-3p. Therefore, these results provide evidence of the capacity of dietary proanthocyanidins to influence microRNA expression, suggesting a new mechanism of action of proanthocyanidins.

  18. Study the radiation damage effects in Si microstrip detectors for future HEP experiments

    International Nuclear Information System (INIS)

    Lalwani, Kavita; Jain, Geetika; Dalal, Ranjeet; Ranjan, Kirti; Bhardwaj, Ashutosh

    2016-01-01

    Silicon (Si) detectors are playing a key role in High Energy Physics (HEP) experiments due to their superior tracking capabilities. In future HEP experiments, like upgrade of the Compact Muon Solenoid (CMS) experiment at the Large Hadron Collider (LHC), CERN, the silicon tracking detectors will be operated in a very intense radiation environment. This leads to both surface and bulk damage in Si detectors, which in turn will affect the operating performance of Si detectors. It is important to complement the measurements of the irradiated Si strip detectors with device simulation, which helps in understanding of both the device behavior and optimizing the design parameters needed for the future Si tracking system. An important ingredient of the device simulation is to develop a radiation damage model incorporating both bulk and surface damage. In this work, a simplified two-trap model is incorporated in device simulation to describe the type-inversion. Further, an extensive simulation of effective doping density as well as electric field profile is carried out at different temperatures for various fluences.

  19. Study the radiation damage effects in Si microstrip detectors for future HEP experiments

    Energy Technology Data Exchange (ETDEWEB)

    Lalwani, Kavita, E-mail: kavita.phy@mnit.ac.in [Malaviya National Institute of Technology (MNIT) Jaipur, Jaipur-302017 (India); Jain, Geetika; Dalal, Ranjeet; Ranjan, Kirti; Bhardwaj, Ashutosh [University of Delhi (DU), Delhi-110007 (India)

    2016-07-15

    Silicon (Si) detectors are playing a key role in High Energy Physics (HEP) experiments due to their superior tracking capabilities. In future HEP experiments, like upgrade of the Compact Muon Solenoid (CMS) experiment at the Large Hadron Collider (LHC), CERN, the silicon tracking detectors will be operated in a very intense radiation environment. This leads to both surface and bulk damage in Si detectors, which in turn will affect the operating performance of Si detectors. It is important to complement the measurements of the irradiated Si strip detectors with device simulation, which helps in understanding of both the device behavior and optimizing the design parameters needed for the future Si tracking system. An important ingredient of the device simulation is to develop a radiation damage model incorporating both bulk and surface damage. In this work, a simplified two-trap model is incorporated in device simulation to describe the type-inversion. Further, an extensive simulation of effective doping density as well as electric field profile is carried out at different temperatures for various fluences.

  20. Taurine reduces the secretion of apolipoprotein B100 and lipids in HepG2 cells

    Directory of Open Access Journals (Sweden)

    Nagao Koji

    2008-10-01

    Full Text Available Abstract Background Higher concentrations of serum lipids and apolipoprotein B100 (apoB are major individual risk factors of atherosclerosis and coronary heart disease. Therefore ameliorative effects of food components against the diseases are being paid attention in the affluent countries. The present study was undertaken to investigate the effect of taurine on apoB secretion and lipid metabolism in human liver model HepG2 cells. Results The results demonstrated that an addition of taurine to the culture media reduces triacylglycerol (TG-mass in the cells and the medium. Similarly, cellular cholesterol-mass was decreased. Taurine inhibited the incorporation of [14C] oleate into cellular and medium TG, suggesting the inhibition of TG synthesis. In addition, taurine reduced the synthesis of cellular cholesterol ester and its secretion, suggesting the inhibition of acyl-coenzyme A:cholesterol acyltransferase activity. Furthermore, taurine reduced the secretion of apoB, which is a major protein component of very low-density lipoprotein. Conclusion This is a first report to demonstrate that taurine inhibits the secretion of apoB from HepG2 cells.

  1. Total Ionizing Dose effects in 130-nm commercial CMOS technologies for HEP experiments

    CERN Document Server

    Gonella, L; Silvestri, M; Gerardin, S; Pantano, D; Re, V; Manghisoni, M; Ratti, L; Ranieri, A

    2007-01-01

    The impact of foundry-to-foundry variability and bias conditions during irradiation on the Total Ionizing Dose (TID) response of commercial 130-nm CMOS technologies have been investigated for applications in High Energy Physics (HEP) experiments. n- and p-channel MOSFETs from three different manufacturers have been irradiated with X-rays up to more than 100 Mrad (SiO2). Even though the effects of TID are qualitatively similar, the amount of degradation is shown to vary considerably from foundry to foundry, probably depending on the processing of the STI oxide and/or doping profile in the substrate. The bias during irradiation showed to have a strong impact as well on the TID response, proving that exposure at worst case bias conditions largely overestimates the degradation a device may experience during its lifetime. Overall, our results increase the confidence that 130-nm CMOS technologies can be used in future HEP experiments even without Hardness-By-Design solutions, provided that constant monitoring of th...

  2. Metabolic Flux Distribution during Defatting of Steatotic Human Hepatoma (HepG2 Cells

    Directory of Open Access Journals (Sweden)

    Gabriel Yarmush

    2016-01-01

    Full Text Available Methods that rapidly decrease fat in steatotic hepatocytes may be helpful to recover severely fatty livers for transplantation. Defatting kinetics are highly dependent upon the extracellular medium composition; however, the pathways involved are poorly understood. Steatosis was induced in human hepatoma cells (HepG2 by exposure to high levels of free fatty acids, followed by defatting using plain medium containing no fatty acids, or medium supplemented with a cocktail of defatting agents previously described before. We measured the levels of 28 extracellular metabolites and intracellular triglyceride, and fed the data into a steady-state mass balance model to estimate strictly intracellular fluxes. We found that during defatting, triglyceride content decreased, while beta-oxidation, the tricarboxylic acid cycle, and the urea cycle increased. These fluxes were augmented by defatting agents, and even more so by hyperoxic conditions. In all defatting conditions, the rate of extracellular glucose uptake/release was very small compared to the internal supply from glycogenolysis, and glycolysis remained highly active. Thus, in steatotic HepG2 cells, glycolysis and fatty acid oxidation may co-exist. Together, these pathways generate reducing equivalents that are supplied to mitochondrial oxidative phosphorylation.

  3. Tendencia de la incidencia de los tumores hepáticos en la infancia

    Directory of Open Access Journals (Sweden)

    Mejía-Aranguré Juan Manuel

    2002-01-01

    Full Text Available Objetivo. Evaluar la tendencia de la incidencia de los diferentes tumores hepáticos en niños residentes en el Distrito Federal. Material y métodos. Encuesta hospitalaria. Se realizó un análisis de dos bases de datos. La primera tiene registrados todos los casos de tumores hepáticos que se encontraron entre el periodo de l982 a 1991, de hospitales que atienden a niños con cáncer, residentes en la Ciudad de México. La segunda base de datos tiene registrados todos los casos de tumores hepáticos que se encontraron de 1996 a 1999 en el Hospital de Pediatría Centro Médico Nacional (CMN "Siglo XXI" y en el Hospital General del Centro Médico Nacional "La Raza", del Instituto Mexicano del Seguro Social (IMSS. Se calcularon las tasas de incidencia anual promedio (TIAP por cada tipo de tumor hepático. Las tasas fueron estandarizadas por el método directo, usando como población de referencia a la mundial estándar menor de l5 años. La tendencia se evaluó con las tasas de incidencia anuales y se calculó la tasa de cambio promedio que emplea la distribución de Poisson. Resultados. Durante el periodo de 1982 a 1991 la TIAP para hepatoblastoma fue el triple en hombres con 0.6 x 10(6. El grupo más afectado fue el de 1 a 4 años.(Para los hepatocarcinomas la TIAP fue de 0.14 para el sexo femenino, siendo el doble que la del sexo masculino. Para el periodo de 1996 a 1999 la TIAP para hepatoblastomas fue de 5.11 en mujeres y de 1.85 en hombres. El grupo de edad con la tasa más alta fue el de mujeres menores de un año. Para hepatocarcinomas la TIAP fue de 0.64 para hombres y de 1.23 en mujeres. El grupo de edad más afectado fue el de hombres de 10 a 14 años. No se observó tendencia significativa al incremento o decremento en la incidencia de hepatoblastomas. Para hepatocarcinomas hubo una tasa de cambio de 10%, pero tampoco fue significativa. Conclusiones. No existe en la Ciudad de México una tendencia en la incidencia de los tumores hep

  4. Habitat Evaluation Procedures (HEP) Report : Hellsgate Project, 1999-2000 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Berger, Matthew

    2000-05-01

    A Habitat Evaluation Procedure (HEP) study was conducted on lands acquired and/or managed (4,568 acres total) by the Hellsgate Big Game Winter Range Wildlife Mitigation Project (Hellsgate project) to mitigate some of the losses associated with the original construction and operation of Grand Coulee Dam and inundation of habitats behind the dams. Three separate properties, totaling 2,224 acres were purchased in 1998. One property composed of two separate parcels, mostly grassland lies southeast of the town of Nespelem in Okanogan County (770 acres) and was formerly called the Hinman property. The former Hinman property lies within an area the Tribes have set aside for the protection and preservation of the sharp-tailed grouse (Agency Butte unit). This special management area minus the Hinman acquisition contains 2,388 acres in a long-term lease with the Tribes. The second property lies just south of the Silver Creek turnoff (Ferry County) and is bisected by the Hellsgate Road (part of the Friedlander unit). This parcel contains 60 acres of riparian and conifer forest cover. The third property (now named the Sand Hills unit) acquired for mitigation (1,394 acres) lies within the Hellsgate Reserve in Ferry County. This new acquisition links two existing mitigation parcels (the old Sand Hills parcels and the Lundstrum Flat parcel, all former Kuehne purchases) together forming one large unit. HEP team members included individuals from the Colville Confederated Tribes Fish and Wildlife Department (CTCR), Washington Department of Fish and Wildlife (WDFW), and Bureau of Land Management (BLM). The HEP team conducted a baseline habitat survey using the following HEP species models: mule deer (Odocoileus hemionus), mink (Mustela vison), downy woodpecker (Picoides pubescens), bobcat (Lynx rufus), yellow warbler (Dendroica petechia), and sharp-tailed grouse (Tympanuchus phasianellus columbianus). HEP analysis and results are discussed within the body of the text. The cover types

  5. Antiproliferative activity and phenotypic modification induced by selected Peruvian medicinal plants on human hepatocellular carcinoma Hep3B cells.

    Science.gov (United States)

    Carraz, Maëlle; Lavergne, Cédric; Jullian, Valérie; Wright, Michel; Gairin, Jean Edouard; Gonzales de la Cruz, Mercedes; Bourdy, Geneviève

    2015-05-26

    The high incidence of human hepatocellular carcinoma (HCC) in Peru and the wide use of medicinal plants in this country led us to study the activity against HCC cells in vitro of somes species used locally against liver and digestive disorders. Ethnopharmacological survey: Medicinal plant species with a strong convergence of use for liver and digestive diseases were collected fresh in the wild or on markets, in two places of Peru: Chiclayo (Lambayeque department, Chiclayo province) and Huaraz (Ancash department, Huaraz province). Altogether 51 species were collected and 61 ethanol extracts were prepared to be tested. Biological assessment: All extracts were first assessed against the HCC cell line Hep3B according a 3-step multi-parametric phenotypic assay. It included 1) the evaluation of phenotypic changes on cells by light microscopy, 2) the measurement of the antiproliferative activity and 3) the analysis of the cytoskeleton and mitosis by immunofluorescence. Best extracts were further assessed against other HCC cell lines HepG2, PLC/PRF/5 and SNU-182 and their toxicity measured in vitro on primary human hepatocytes. Ethnopharmacological survey: Some of the species collected had a high reputation spreading over the surveyed locations for treating liver problems, i.e. Baccharis genistelloides, Bejaria aestuans, Centaurium pulchellum, Desmodium molliculum, Dipsacus fullonum, Equisetum bogotense, Gentianella spp., Krameria lapacea, Otholobium spp., Schkuhria pinnata, Taraxacum officinale. Hep3B evaluation: Fourteen extracts from 13 species (Achyrocline alata, Ambrosia arborescens, Baccharis latifolia, Hypericum laricifolium, Krameria lappacea, Niphidium crassifolium, Ophryosporus chilca, Orthrosanthus chimboracensis, Otholobium pubescens, Passiflora ligularis, Perezia coerulescens, Perezia multiflora and Schkuhria pinnata) showed a significant antiproliferative activity against Hep3B cells (IC50≤ 50µg/mL). This was associated with a lack of toxicity on primary

  6. Avaliação dos tumores hepáticos ao Doppler Doppler evaluation of liver tumors

    OpenAIRE

    Márcio Martins Machado; Ana Cláudia Ferreira Rosa; Paulo Herman; Nestor de Barros; Orlando Milhomem da Mota; Letícia Martins Azeredo; Osterno Queiroz da Silva; Jales Benevides Santana Filho; Paulo Moacir O. Campoli; Paulo Adriano Q. Barreto; Patrícia Medeiros Milhomem; Leonardo Medeiros Milhomem; Murilo Tavares Daher; Renato Tavares Daher; Luciana Mendes de Oliveira Cerri

    2004-01-01

    Os avanços recentes na ultra-sonografia têm ampliado a possibilidade de detecção de tumores hepáticos. Isto tem auxiliado na perspectiva de melhora do prognóstico destes pacientes, à medida que novas técnicas terapêuticas têm surgido. Neste artigo os autores relatam achados ao Doppler que podem auxiliar na identificação e caracterização dos tumores hepáticos, avaliando dados do Doppler colorido, pulsado e do Doppler de amplitude ("power Doppler"). Fazem, também, referência a novas modalidades...

  7. Data on HepG2 cells changes following exposure to cadmium sulphide quantum dots (CdS QDs

    Directory of Open Access Journals (Sweden)

    Laura Paesano

    2017-04-01

    Full Text Available The data included in this paper are associated with the research article entitled "Markers for toxicity to HepG2 exposed to cadmium sulphide quantum dots; damage to mitochondria" (Paesano et al. [1]. The article concerns the cytotoxic and genotoxic effects of CdS QDs in HepG2 cells and the mechanisms involved. In this dataset, changes in expression levels of candidate genes are reported, together with details concerning synthesis and properties of CdS QDs, additional information obtained through literature survey, measures of the mitochondrial membrane potential and the glutathione redox state.

  8. Adenomatose hepática múltipla: diagnóstico pela ultra-sonografia intra-operatória (USIO. Nota prévia Multiple hepatic adenomatosis: diagnosis by intraoperative ultrasonography (IOUS. Original communication

    Directory of Open Access Journals (Sweden)

    Márcio Martins Machado

    2001-12-01

    Full Text Available Os autores relatam um caso de adenomatose hepática múltipla diagnosticado pela ultra-sonografia intra-operatória (USIO. Neste caso foram identificadas 13 lesões pela USIO. Os exames pré-operatórios (ultra-sonografia abdominal, tomografia computadorizada helicoidal e ressonância magnética mostraram apenas três lesões, que foram inicialmente consideradas potencialmente ressecáveis. Todos os adenomas apresentavam consistência idêntica à do fígado adjacente, não sendo, portanto, perceptíveis à palpação. A USIO apresenta maior acurácia na identificação de nódulos hepáticos, quando comparada com os exames pré-operatórios. No presente estudo os autores consideraram a USIO fundamental no estabelecimento do diagnóstico, identificando os múltiplos adenomas, e que também auxiliou mudando a conduta cirúrgica da paciente. Devido aos múltiplos adenomas identificados pela USIO, a paciente não foi submetida à ressecção hepática, pois não haveria a possibilidade de remoção de todas as lesões. No conhecimento dos autores, ainda não havia sido descrito caso de adenomatose hepática múltipla diagnosticada apenas pela USIO.The authors report a case of a patient with multiple hepatic adenomas diagnosed by intraoperative ultrasonography (IOUS. Thirteen lesions were observed by IOUS. Preoperative abdominal sonography, helical computed tomography and magnetic resonance imaging revealed only three lesions, which were initially considered to be resectable. All hepatic adenomas had the same texture of the hepatic parenchyma and were not detected by palpation. IOUS is considered to have greater accuracy in identifying hepatic nodules than preoperative imaging methods. In the present study, the authors considered that IOUS was essential for the diagnosis of multiple adenomas, and allowed changing the surgical approach. Because of the multiple lesions identified by IOUS, the patient was not eligible for surgical resection as it would

  9. [Effects of conditioned media for rat bone marrow-derived mesenchymal stem cells on palmitic acid-induced insulin resistance in HepG2 cells].

    Science.gov (United States)

    Sun, Xiaoya; Hao, Haojie; Han, Weidong; Mu, Yiming

    2015-05-01

    To study the effect of conditioned media for rat bone marrow mesenchymal stem cells (BMSCs-CM) on palmitic acid (PA)-induced insulin resistance (IR) in HepG2 cells and its underlying molecular mechanisms. HepG2 cells were treated with or without BMSCs-CM and L-DMEM in the presence or absence of PA.Glucose utilization in HepG2 cells were detected with PAS, glucose and glycogen measurements. Western blotting was used to assess the expression of phospho-insulin receptor substrate (p-IRS), phosphatidylinositol 3-kinase (PI3K) and p-AKT. (1) Incubation of HepG2 cells with 0.25 mmol/L PA for 24 hours significantly increased the glucose concentration and decreased the glycogen content (Palteration in cells pretreated with PA (Pinsulin sensitivity in HepG2 cells pretreated with PA through upregulation of insulin signaling component expression.

  10. Habitat Evaluation Procedures (HEP) Report; Iskuulpa Wildlife Mitigation and Watershed Project, Technical Report 1998-2003.

    Energy Technology Data Exchange (ETDEWEB)

    Quaempts, Eric

    2003-01-01

    U.S. Fish and Wildlife Service (USFWS) Habitat Evaluation Procedures (HEP) were used to determine the number of habitat units credited to evaluate lands acquired and leased in Eskuulpa Watershed, a Confederated Tribes of the Umatilla Indian Reservation watershed and wildlife mitigation project. The project is designed to partially credit habitat losses incurred by BPA for the construction of the John Day and McNary hydroelectric facilities on the Columbia River. Upland and riparian forest, upland and riparian shrub, and grasslands cover types were included in the evaluation. Indicator species included downy woodpecker (Picuides puhescens), black-capped chickadee (Pams atricopillus), blue grouse (Beadragapus obscurus), great blue heron (Ardea herodias), yellow warbler (Dendroica petschia), mink (Mustela vison), and Western meadowlark (Sturnello neglects). Habitat surveys were conducted in 1998 and 1999 in accordance with published HEP protocols and included 55,500 feet of transects, 678 m2 plots, and 243 one-tenth-acre plots. Between 123.9 and f 0,794.4 acres were evaluated for each indicator species. Derived habitat suitability indices were multiplied by corresponding cover-type acreages to determine the number of habitat units for each species. The total habitat units credited to BPA for the Iskuulpa Watershed Project and its seven indicator species is 4,567.8 habitat units. Factors limiting habitat suitability are related to the direct, indirect, and cumulative effects of past livestock grazing, road construction, and timber harvest, which have simplified the structure, composition, and diversity of native plant communities. Alternatives for protecting and improving habitat suitability include exclusion of livestock grazing or implementation of restoration grazing schemes, road de-commissioning, reforestation, large woody debris additions to floodplains, control of competing and unwanted vegetation, reestablishing displaced or reduced native vegetation species

  11. Valoración no invasiva de la sobrecarga hepática de hierro en pacientes con hemocromatosis

    Directory of Open Access Journals (Sweden)

    J.C. Spina

    2014-01-01

    La RM es fundamental en el diagnóstico de la hemocromatosis, especialmente en la fase subclínica. Contribuye a definir la severidad de la sobrecarga de hierro hepático y a valorar la respuesta al tratamiento, evitando procedimientos invasivos.

  12. Effects of the radiolysis products of sennoside A on HepG2 and PC-3 cell

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dong Ho; Jo, Min Ho [Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup (Korea, Republic of)

    2016-11-15

    Radiolysis of sennoside A was carried out by gamma irradiation and the anti-cancer activities of the radiolysis product were evaluated. An aqueous solution of sennoside A was exposed to 0.5-3 kGy of gamma irradiation and the radiolysis products were analyzed by HPLC. A fraction of radiolysis product (RLF) of sennoside A was isolated and the RLF was presumed as a rhein-8-β-D-glucoside. The anticancer effect of the RLF was compared with the sennoside and rhein using a in vitro assay system of human prostate cancer cells (PC-3) and human hepatoma HepG2 cells. The cell viability of PC-3 and HepG2 cell was significantly decreased to 12.4±1.2% and 32.4±2.1%, respectively, by the treatment of 0.6 μM of RLF. The sennoside A (range from 0 to 25 μM) had no cytotoxic effect on PC-3 and HepG2 cells, while the rhein had the effect on HepG2 cells with a LD{sub 50} at 80 μM.

  13. Caracterización inmunohistoquímica de los tumores hepáticos en el perro

    OpenAIRE

    Espinosa de los Monteros y Zayas, Antonio Rafae

    1996-01-01

    [ES] Se investiga si pueden ser demostrados algunos marcadores tumorales, que habitualmente se emplean en el diagnóstico patológico humano, en muestras de tumores hepáticos caninos procesadas rutinariamente y utilizando métodos inmunohistoquímicos.

  14. Heparan sulfate chains from glypican and syndecans bind the Hep II domain of fibronectin similarly despite minor structural differences

    DEFF Research Database (Denmark)

    Tumova, S; Woods, A; Couchman, J R

    2000-01-01

    syndecan-4. Despite distinct molecular masses of glypican and syndecan glycosaminoglycans and minor differences in disaccharide composition and sulfation pattern, the overall proportion and distribution of sulfated regions and the affinity for the Hep II domain were similar. Therefore, adhesion regulation...

  15. Up-regulation of P-glycoprotein expression by catalase via JNK activation in HepG2 cells.

    Science.gov (United States)

    Li, Lin; Xu, Jianfeng; Min, Taishan; Huang, Weida

    2006-01-01

    Overexpression of the MDR1 gene is one of the reasons for multidrug resistance (MDR). Some studies suggested that antioxidants could down-regulate MDR1 expression as a possible cancer treatment. In this report, we try to determine the effects of antioxidants (catalase or N-acetylcysteine [NAC]) on the regulation of intrinsic MDR1 overexpression in HepG2 cells. Adding catalase or N-acetylcysteine to the HepG2 culture led to a significant increase of MDR1 mRNA and P-glycoprotein drug transporter activity. After catalase or NAC treatment, a reduced intracellular reactive oxygen species (ROS) was observed. The JNK inhibitor SP600125 abolished the positive effects of catalase on drug transporter activity in a dose-dependent manner. Furthermore, the up-regulation of P-glycoprotein functions by catalase was only observed in HepG2 cells but not in other cell lines tested (MCF-7, A549, A431). These data suggested that catalase can up-regulate P-glycoprotein expression in HepG2 cells via reducing intracellular ROS, and JNK may mediate this process.

  16. HEPS Inventory Tool: An Inventory Tool Including Quality Assessment of School Interventions on Healthy Eating and Physical Activity

    Science.gov (United States)

    Dadaczynski, Kevin; Paulus, Peter; de Vries, Nanne; de Ruiter, Silvia; Buijs, Goof

    2010-01-01

    The HEPS Inventory Tool aims to support stakeholders working in school health promotion to promote high quality interventions on healthy eating and physical activity. As a tool it provides a step-by-step approach on how to develop a national or regional inventory of existing school based interventions on healthy eating and physical activity. It…

  17. Flavonoids of Korean Citrus aurantium L. Induce Apoptosis via Intrinsic Pathway in Human Hepatoblastoma HepG2 Cells.

    Science.gov (United States)

    Lee, Seung Hwan; Yumnam, Silvia; Hong, Gyeong Eun; Raha, Suchismita; Saralamma, Venu Venkatarame Gowda; Lee, Ho Jeong; Heo, Jeong Doo; Lee, Sang Joon; Lee, Won-Sup; Kim, Eun-Hee; Park, Hyeon Soo; Kim, Gon Sup

    2015-12-01

    Korean Citrus aurantium L. has long been used as a medicinal herb for its anti-inflammatory, antioxidant, and anticancer properties. The present study investigates the anticancer role of flavonoids extracted from C. aurantium on human hepatoblastoma cell, HepG2. The Citrus flavonoids inhibit the proliferation of HepG2 cells in a dose-dependent manner. This result was consistent with the in vivo xenograft results. Apoptosis was detected by cell morphology, cell cycle analysis, and immunoblot. Flavonoids decreased the level of pAkt and other downstream targets of phosphoinositide-3-kinase/Akt pathway - P-4EBP1 and P-p70S6K. The expressions of cleaved caspase 3, Bax, and Bak were increased, while those of Bcl-2 and Bcl-xL were decreased with an increase in the expression of Bax/Bcl-xL ratio in treated cells. Loss of mitochondrial membrane potential was also observed in flavonoid-treated HepG2 cells. It was also observed that the P-p38 protein level was increased both dose and time dependently in flavonoid-treated cells. Collectively, these results suggest that flavonoid extracted from Citrus inhibits HepG2 cell proliferation by inducing apoptosis via an intrinsic pathway. These findings suggest that flavonoids extracted from C. aurantium L. are potential chemotherapeutic agents against liver cancer. Copyright © 2015 John Wiley & Sons, Ltd.

  18. Quercetin protects human hepatoma HepG2 against oxidative stress induced by tert-butyl hydroperoxide

    International Nuclear Information System (INIS)

    Alia, Mario; Ramos, Sonia; Mateos, Raquel; Granado-Serrano, Ana Belen; Bravo, Laura; Goya, Luis

    2006-01-01

    Flavonols such as quercetin, have been reported to exhibit a wide range of biological activities related to their antioxidant capacity. The objective of the present study was to investigate the protective effect of quercetin on cell viability and redox status of cultured HepG2 cells submitted to oxidative stress induced by tert-butyl hydroperoxide. Concentrations of reduced glutathione and malondialdehyde, generation of reactive oxygen species and activity and gene expression of antioxidant enzymes were used as markers of cellular oxidative status. Pretreatment of HepG2 with 10 μM quercetin completely prevented lactate dehydrogenase leakage from the cells. Pretreatment for 2 or 20 h with all doses of quercetin (0.1-10 μM) prevented the decrease of reduced glutathione and the increase of malondialdehyde evoked by tert-butyl hydroperoxide in HepG2 cells. Reactive oxygen species generation induced by tert-butyl hydroperoxide was significantly reduced when cells were pretreated for 2 or 20 h with 10 μM and for 20 h with 5 μM quercetin. Finally, some of the quercetin treatments prevented the significant increase of glutathione peroxidase, superoxide dismutase, glutathione reductase and catalase activities induced by tert-butyl hydroperoxide. Gene expression of antioxidant enzymes was also affected by the treatment with the polyphenol. The results of the biomarkers analyzed clearly show that treatment of HepG2 cells in culture with the natural dietary antioxidant quercetin strongly protects the cells against an oxidative insult

  19. Garcinia dulcis Fruit Extract Induced Cytotoxicity and Apoptosis in HepG2 Liver Cancer Cell Line

    Directory of Open Access Journals (Sweden)

    Mohd Fadzelly Abu Bakar

    2015-01-01

    Full Text Available Garcinia dulcis or locally known in Malaysia as “mundu” belongs to the family of Clusiaceae. The study was conducted to investigate the anticancer potential of different parts of G. dulcis fruit extracts and their possible mechanism of action in HepG2 liver cancer cell line. MTT assay showed that the peel, flesh, and seed extracts of G. dulcis induced cytotoxicity in HepG2 cell line with IC50 values of 46.33 ± 4.51, 38.33 ± 3.51, and 7.5 ± 2.52 µg/mL, respectively. The flesh extract of G. dulcis induced cell cycle arrest at sub-G1 (apoptosis phase in a time-dependent manner. Staining with Annexin V-FITC and propidium iodide showed that 41.2% of the cell population underwent apoptosis after 72 hours of exposure of the HepG2 cell line to G. dulcis flesh extract. Caspase-3 has been shown to be activated which finally leads to the death of HepG2 cell (apoptosis. GC-MS analysis showed that the highest percentage of compound identified in the extract of G. dulcis flesh was hydroxymethylfurfural and 3-methyl-2,5-furandione, together with xanthones and flavonoids (based on literature, could synergistically contribute to the observed effects. This finding suggested that the flesh extract of G. dulcis has its own potential as cancer chemotherapeutic agent against liver cancer cell.

  20. Biochemical Effects of six Ti02 and four Ce02 Nanomaterials in HepG2 cells

    Science.gov (United States)

    Abstract The potential mammalian hepatotoxicity of nanomaterials were explored in dose-response and structure-activity studies with human hepatic HepG2 cells exposed to between 10 and 1000 ug/ml of six different TiO2 and four CeO2 nanomaterials for 3 days. Var...

  1. Differential genomic effects on signaling pathways by two different CeO2 nanoparticles in HepG2 cells

    Science.gov (United States)

    To investigate genomic effects, human liver hepatocellular carcinoma (HepG2) cells were exposed for three days to two different forms of nanoparticles both composed of Ce02 (0.3, 3 and 30 µg/mL). The two Ce02 nanopartices had dry primary particle sizes of 8 nanometers {(M) made b...

  2. Anatomia das vias biliares em doadores e receptores de transplante hepático inter vivos Study of biliary anatomy in living related liver transplantation

    Directory of Open Access Journals (Sweden)

    Renato Vianna Soares

    2006-09-01

    Full Text Available RACIONAL: Variações anatômicas das vias biliares são freqüentes e aumentam as complicações dos transplantes hepáticos. OBJETIVO: Avaliar a anatomia das vias biliares nos doadores e receptores do transplante hepático inter vivos realizados no Hospital de Clínicas da Universidade Federal do Paraná e do Hospital Nossa Senhora das Graças de Curitiba, Curitiba, PR. CASUÍSTICA E MÉTODOS: A avaliação foi retrospectiva de março de 1998 até setembro de 2002 (23 transplantes, quando os dados passaram a ser captados de forma prospectiva (17 transplantes, até agosto de 2003. Foram obtidos dados de 80 pacientes consecutivos (40 doadores e 40 receptores, submetidos a transplante hepático inter vivos, sendo 32 transplantes com receptor adulto e 8 transplantes pediátricos (receptor com idade inferior a 15 anos. Entre os 80 pacientes incluídos no estudo, 51 eram do sexo masculino (27 receptores e 24 doadores e 29 do feminino (13 receptores e 16 doadores. A idade média dos doadores foi de 32,6 anos e a dos receptores de 36,3 anos. RESULTADOS: Nas cirurgias de captação do lobo hepático direito, a via biliar direita foi única em 25 entre 29 casos (86,2%, dupla em dois (6,9% e tripla em dois (6,9%. Nos oito casos em que o setor lateral esquerdo ou o lobo hepático esquerdo foram captados, a via biliar foi única. Entre os 33 pacientes submetidos a colangiografia por ressonância magnética, variações foram verificadas em três pacientes (9,1%, sendo a mais comum o ducto biliar posterior direito tributário do ducto hepático esquerdo (6,06%; n = 2. CONCLUSÃO: A prevalência de anomalias biliares nos transplantes inter vivos de fígado é elevada e algumas não são diagnosticadas com os exames de imagem pré-operatórios.BACKGROUND: Anatomic variations of the biliary tree are frequent and increase complications after liver transplantation. AIM: To describe the anatomy of the bile ducts of donors and recipients of living related liver

  3. Cytotoxicity assessments of Portulaca oleracea and Petroselinum sativum seed extracts on human hepatocellular carcinoma cells (HepG2).

    Science.gov (United States)

    Farshori, Nida Nayyar; Al-Sheddi, Ebtesam Saad; Al-Oqail, Mai Mohammad; Musarrat, Javed; Al-Khedhairy, Abdulaziz Ali; Siddiqui, Maqsood Ahmed

    2014-01-01

    The Pharmacological potential, such as antioxidant, anti-inflammatory, and antibacterial activities of Portulaca oleracea (PO) and Petroselinum sativum (PS) extracts are well known. However, the preventive properties against hepatocellular carcinoma cells have not been explored so far. Therefore, the present investigation was designed to study the anticancer activity of seed extracts of PO and PS on the human hepatocellular carcinoma cells (HepG2). The HepG2 cells were exposed with 5-500 μg/ml of PO and PS for 24 h. After the exposure, cell viability by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay, neutral red uptake (NRU) assay, and cellular morphology by phase contrast inverted microscope were studied. The results showed that PO and PS extracts significantly reduced the cell viability of HepG2 in a concentration dependent manner. The cell viability was recorded to be 67%, 31%, 21%, and 17% at 50, 100, 250, and 500 μg/ml of PO, respectively by MTT assay and 91%, 62%, 27%, and 18% at 50, 100, 250, and 500 μg/ml of PO, respectively by NRU assay. PS exposed HepG2 cells with 100 μg/ml and higher concentrations were also found to be cytotoxic. The decrease in the cell viability at 100, 250, and 500 μg/ml of PS was recorded as 70%, 33%, and 15% by MTT assay and 63%, 29%, and 17%, respectively by NRU assay. Results also showed that PO and PS exposed cells reduced the normal morphology and adhesion capacity of HepG2 cells. HepG2 cells exposed with 50 μg/ml and higher concentrations of PO and PS lost their typical morphology, become smaller in size, and appeared in rounded bodies. Our results demonstrated preliminary screening of anticancer activity of Portulaca oleracea and Petroselinum sativum extracts against HepG2 cells, which can be further used for the development of a potential therapeutic anticancer agent.

  4. Rotura hepática espontânea na síndrome HELLP

    OpenAIRE

    Freitas,Alexandre Coutinho Teixeira de; Schulz,Gustavo; Mori,Rafael; Coelho,Julio Cezar Uili

    2009-01-01

    INTRODUÇÃO: A síndrome HELLP, condição com sinais de hemólise, elevação de enzimas hepáticas e baixa contagem de plaquetas, é situação obstétrica que pode apresentar severas complicações para a mãe, incluindo hematoma subcapsular com risco de ruptura para a cavidade peritoneal. RELATO DO CASO: Um caso de ruptura espontânea do fígado secundária a síndrome HELLP é apresentado. A paciente foi submetida à laparotomia exploradora devido a choque hemorrágico. CONCLUSÃO: A síndrome HELLP é condição ...

  5. Interaction between the P1 protein of Mycoplasma pneumoniae and receptors on HEp-2 cells

    DEFF Research Database (Denmark)

    Drasbek, Mette; Christiansen, Gunna; Drasbek, Kim Ryun

    2007-01-01

    The human pathogen Mycoplasma pneumoniae can cause atypical pneumonia through adherence to epithelial cells in the respiratory tract. The major immunogenic protein, P1, participates in the attachment of the bacteria to the host cells. To investigate the adhesion properties of P1, a recombinant...... protein (rP1-II) covering amino acids 1107-1518 of the P1 protein was produced. This protein inhibited the adhesion of M. pneumoniae to human HEp-2 cells, as visualized in a competitive-binding assay using immunofluorescence microscopy. Previous studies have shown that mAbs that recognize two epitopes...... in this region of P1 also reduce M. pneumoniae adhesion. Therefore, peptides covering these epitopes, of 8 and 13 aa, respectively, were synthesized to further investigate the adhesion region. None of these synthetic peptides reduced the binding of M. pneumoniae to the receptors on the host cells. Instead, 10...

  6. Fiber Bragg Grating sensors for deformation monitoring of GEM foils in HEP detectors

    CERN Document Server

    AUTHOR|(CDS)2071648; Bianco, S; Caponero, M; Muhammad, S; Passamonti, L; Piccolo, D; Pierluigi, D; Raffone, G; Russo, A; Saviano, G

    2015-01-01

    Fiber Bragg Grating (FBG) sensors have been so far mainly used in high energy physics (HEP) as high precision positioning and re-positioning sensors and as low cost, easy to mount, radiation hard and low space- consuming temperature and humidity devices. FBGs are also commonly used for very precise strain measurements. In this work we present a novel use of FBGs as flatness and mechanical tensioning sensors applied to the wide Gas Electron Multiplier (GEM) foils of the GE1/1 chambers of the Compact Muon Solenoid (CMS) experiment at Large Hadron Collider (LHC) of CERN. A network of FBG sensors has been used to determine the optimal mechanical tension applied and to characterize the mechanical stress applied to the foils. The preliminary results of the test performed on a full size GE1/1 final prototype and possible future developments will be discussed.

  7. Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

    Directory of Open Access Journals (Sweden)

    Maria del Rosario Espinoza-Mellado

    2013-12-01

    Full Text Available Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a “vacuolating factor” in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae .

  8. 4He(p,2p)3H reaction at intermediate energies

    International Nuclear Information System (INIS)

    van Oers, W.T.H.; Murdoch, B.T.; Koene, B.K.S.; Hasell, D.K.; Abegg, R.; Margaziotis, D.J.; Epstein, M.B.; Moss, G.A.; Greeniaus, L.G.; Greben, J.M.; Cameron, J.M.; Rogers, J.G.; Stetz, A.W.

    1982-01-01

    The 4 He(p,2p) 3 He reaction has been studied at 250, 350, and 500 MeV using coplanar symmetric and asymmetric geometries. The data are presented as energy-sharing spectra, coplanar symmetric angular distributions, and quasifree angular distributions. A comparison with distorted-wave impulse approximation calculations indicates reasonable agreement for small recoil momenta (q< or approx. =150 MeV/c). For larger recoil momenta, the distorted-wave impulse approximation calculations increasingly underestimate the data. The discrepancies are substantially reduced by inclusion of a spin-orbit term in the optical potential used to generate the distorted waves. Improvements of the single particle wave function for the struck nucleon influence the calculations to a lesser degree. The remaining discrepancies at large recoil momenta may, in part be ascribed to multiple scattering effects and exchange processes not included in the standard distorted-wave impulse approximation

  9. GEANT 4: an Object-Oriented toolkit for simulation in HEP

    CERN Multimedia

    Kent, P; Sirotenko, V; Komogorov, M; Pavliouk, A; Greeniaus, G L; Kayal, P I; Routenburg, P; Tanaka, S; Duellmann, D; Innocente, V; Paoli, S; Ranjard, F; Riccardi, F; Ruggier, M; Shiers, J; Egli, S; Kimura, A; Urban, P; Prior, S; Walkden, A; Forti, A; Magni, S; Strahl, K; Kokoulin, R; Braune, K; Volcker, C; Ullrich, T; Takahata, M; Nieminen, P; Ballocchi, G; Mora De Freitas, P; Verderi, M; Rybine, A; Langeveld, W; Nagamatsu, M; Hamatsu, R; Katayama, N; Chuma, J; Felawka, L; Gumplinger, P; Axen, D

    2002-01-01

    %RD44 %title\\\\ \\\\The GEANT4 software has been developed by a world-wide collaboration of about 100 scientists from over 40 institutions and laboratories participating in more than 10 experiments in Europe, Russia, Japan, Canada, and the United States. The GEANT4 detector simulation toolkit has been designed for the next generation of High Energy Physics (HEP) experiments, with primary requirements from the LHC, the CP violation, and the heavy ions experiments. In addition, GEANT4 also meets the requirements from the space and medical communities, thanks to very low energy extensions developed in a joint project with the European Space Agency (ESA). GEANT4 has exploited advanced software engineering techniques (for example PSS-05) and Object-Oriented technology to improve the validation process of the physics results, and in the same time to make possible the distributed software design and development in the world-wide collaboration. Fifteen specialised working groups have been responsible for fields as diver...

  10. Human Error Probabilites (HEPs) for generic tasks and Performance Shaping Factors (PSFs) selected for railway operations

    DEFF Research Database (Denmark)

    Thommesen, Jacob; Andersen, Henning Boje

    at task level, which can be performed with fewer resources than a more detailed analysis of specific errors for each task. The generic tasks are presented with estimated Human Error Probabili-ties (HEPs) based on and extrapolated from the HRA literature, and estimates are compared with samples of measures......This report describes an HRA (Human Reliability Assessment) of six generic tasks and four Perfor-mance Shaping Factors (PSFs) targeted at railway operations commissioned by Banedanmark. The selection and characterization of generic tasks and PSFs are elaborated by DTU Management in close...... on estimates derived from industries other than rail and the general warning that a task-based analysis is less precise than an error-based one. The authors recommend that estimates be adjusted to actual measures of task failures when feasible....

  11. Characterization of dengue virus entry into HepG2 cells

    Directory of Open Access Journals (Sweden)

    Suksanpaisan Lukkana

    2009-02-01

    Full Text Available Abstract Background Despite infections by the dengue virus being a significant problem in tropical and sub-tropical countries, the mechanism by which the dengue virus enters into mammalian cells remains poorly described. Methods A combination of biochemical inhibition, dominant negative transfection of Eps15 and siRNA mediated gene silencing was used to explore the entry mechanism of dengue into HepG2 cells. Results Results were consistent with entry via multiple pathways, specifically via clathrin coated pit mediated endocytosis and macropinocytosis, with clathrin mediated endocytosis being the predominant pathway. Conclusion We propose that entry of the dengue virus to mammalian cells can occur by multiple pathways, and this opens the possibility of the virus being directed to multiple cellular compartments. This would have significant implications in understanding the interaction of the dengue virus with the host cell machinery.

  12. Tratamento local do carcinoma hepatocelular como ponte para o transplante hepático

    Directory of Open Access Journals (Sweden)

    Alexandre Coutinho Teixeira de Freitas

    Full Text Available OBJETIVO: Analisar os resultados da terapia local pré-operatória em pacientes portadores de carcinoma hepatocelular submetidos a transplante hepático. MÉTODOS: . Foram analisados os prontuários dos pacientes adultos submetidos a transplante hepático cadavérico e intervivos no Hospital de Clínicas da Universidade Federal do Paraná no período entre janeiro de 2002 e agosto de 2007. Foram incluídos no estudo os portadores de cirrose hepática e carcinoma hepatocelular diagnosticado pelos critérios da EASL (European Association for the Study of the Liver. Foram analisados o número e o diâmetro dos nódulos neoplásicos antes e após a terapia local e na análise do explante, o número de sessões de terapia local e sua duração. Após o estabelecimento do diagnóstico de carcinoma hepatocelular os pacientes foram submetidos à terapia local com alcoolização. RESULTADOS: Foram analisados 22 pacientes portadores de 31 nódulos neoplásicos com diâmetro médio de 28,8±12 mm. Após as sessões de terapia local foram detectados 29 nódulos neoplásicos com diâmetro médio de 24,6±12 mm, sem diferença em relação ao observado antes do tratamento e todos dentro dos critérios de Milão. Foram realizados 17 transplantes cadavéricos e cinco transplantes intervivos. A análise do explante demonstrou seis casos fora dos critérios de Milão. Dezesseis casos estavam dentro dos critérios de Milão com 14 nódulos neoplásicos com diâmetro médio de 30±14 mm, sem diferença em relação ao observado no diagnóstico e após a terapia local. CONCLUSÃO: A terapia local para o carcinoma hepatocelular com alcoolização e quimioembolização permitiu o controle parcial da evolução da doença considerando-se os critérios de Milão em pacientes em lista de espera para transplante hepático. Ocorreram diferenças significativas em relação aos critérios de Milão entre os exames de imagem pré-operatórios e a análise do explante.

  13. On the relevance of efficient, integrated computer and network monitoring in HEP distributed online environment

    CERN Document Server

    Carvalho, D F; Delgado, V; Albert, J N; Bellas, N; Javello, J; Miere, Y; Ruffinoni, D; Smith, G

    1996-01-01

    Large Scientific Equipments are controlled by Computer System whose complexity is growing driven, on the one hand by the volume and variety of the information, its distributed nature, thhe sophistication of its trearment and, on the over hand by the fast evolution of the computer and network market. Some people call them generically Large-Scale Distributed Data Intensive Information Systems or Distributed Computer Control Systems (DCCS) for those systems dealing more with real time control. Taking advantage of (or forced by) the distributed architecture, the tasks are more and more often implemented as Client-Server applications. In this frame- work the monitoring of the computer nodes, the communications network and the applications becomes of primary importance for ensuring the safe running and guaranteed performance of the system. With the future generation of HEP experiments, such as those at the LHC in view, it is to integrate the various functions of DCCS monitoring into one general purpose Multi-layer ...

  14. WILGA Photonics and Web Engineering, January 2012; EuCARD Sessions on HEP and Accelerator Technology

    CERN Document Server

    Romaniuk, R S

    2012-01-01

    Wilga Sessions on HEP experiments and accelerator technology were organized under the umbrella of the EU FP7 Project EuCARD – European Coordination for Accelerator Research and Development. The paper presents a digest of chosen technical work results shown by young researchers from technical universities during the SPIE-IEEE Wilga January 2012 Symposium on Photonics and Web Engineering. Topical tracks of the symposium embraced, among others, new technologies for photonics, sensory and nonlinear optical fibers, object oriented design of hardware, photonic metrology, optoelectronics and photonics applications, photonics-electronics co-design, optoelectronic and electronic systems for astronomy and high energy physics experiments, JET and pi-of-the sky experiments development. The symposium held two times a year is a summary in the development of numerable Ph.D. theses carried out in this country in the area of advanced electronic and photonic systems. It is also a great occasion for SPIE, IEEE, OSA and PSP st...

  15. Tyrosine kinase/phosphatase inhibitors decrease dengue virus production in HepG2 cells.

    Science.gov (United States)

    Limjindaporn, Thawornchai; Panaampon, Jutatip; Malakar, Shilu; Noisakran, Sansanee; Yenchitsomanus, Pa-Thai

    2017-01-29

    Dengue virus is the causative agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. High rates of dengue virus replication and virion production are related to disease severity. To identify anti-DENV compounds, we performed cell-based ELISA testing to detect the level of DENV E protein expression. Among a total of 83 inhibitors, eight were identified as inhibitors with antiviral activity. Epidermal growth factor receptor inhibitor II (EGFR/ErbB-2/ErbB-4 inhibitor II) and protein tyrosine phosphatase inhibitor IV (PTP inhibitor IV) significantly inhibited dengue virus production and demonstrated low toxicity in hepatocyte cell lines. Our results suggest the efficacy of tyrosine kinase/phosphatase inhibitors in decreasing dengue virus production in HepG2 cells. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Effect of biotin and galactose functionalized gelatin nanofiber membrane on HEp-2 cell attachment and cytotoxicity.

    Science.gov (United States)

    Selvakumar, R; Mohaideen, S Nazar Mohamed; Aravindh, S; Sabarinath, C; Ananthasubramanian, M

    2014-01-01

    In the present study, we prepared a gelatin nanofiber matrix using an electrospinning technique and cross-linked the nanofibers with 10 % glutaraldehyde vapors. The insoluble nanofibers were functionalized with bioactive molecules like biotin (1 %) and galactose (1 %) by adsorption and coelectrospinning. Surface morphology and fiber dimension were analyzed using atomic force microscopy. The amounts of biotin and galactose bound to the nanofibers before and after adsorption were quantified using high-performance liquid chromatography. Human larynx carcinoma (HEp-2) cell attachment, morphology and cytotoxic characteristics were studied using crystal violet staining and the MTT assay. Cell attachment and viability were highest in biotin- and galactose-embedded nanofibers compared to native nanofibers. Cytotoxicity was less with biotin- and galactose-embedded and adsorbed nanofibers compared to control nanofibers. Hence, we suggest that these biocompatible, nontoxic, biodegradable, functionalized nanofibers could be a potential candidate for application in tissue engineering and scaffold preparation.

  17. ProMC: Input-output data format for HEP applications using varint encoding

    Science.gov (United States)

    Chekanov, S. V.; May, E.; Strand, K.; Van Gemmeren, P.

    2014-10-01

    A new data format for Monte Carlo (MC) events, or any structural data, including experimental data, is discussed. The format is designed to store data in a compact binary form using variable-size integer encoding as implemented in the Google's Protocol Buffers package. This approach is implemented in the PROMC library which produces smaller file sizes for MC records compared to the existing input-output libraries used in high-energy physics (HEP). Other important features of the proposed format are a separation of abstract data layouts from concrete programming implementations, self-description and random access. Data stored in PROMC files can be written, read and manipulated in a number of programming languages, such C++, JAVA, FORTRAN and PYTHON.

  18. Prooxidant and antioxidant properties of salicylaldehyde isonicotinoyl hydrazone iron chelators in HepG2 cells.

    Science.gov (United States)

    Caro, Andres A; Commissariat, Ava; Dunn, Caroline; Kim, Hyunjoo; García, Salvador Lorente; Smith, Allen; Strang, Harrison; Stuppy, Jake; Desrochers, Linda P; Goodwin, Thomas E

    2015-11-01

    Salicylaldehyde isonicotinoyl hydrazone (SIH) is an iron chelator of the aroylhydrazone class that displays antioxidant or prooxidant effects in different mammalian cell lines. Because the liver is the major site of iron storage, elucidating the effect of SIH on hepatic oxidative metabolism is critical for designing effective hepatic antioxidant therapies. Hepatocyte-like HepG2 cells were exposed to SIH or to analogs showing greater stability, such as N'-[1-(2-Hydroxyphenyl)ethyliden]isonicotinoyl hydrazide (HAPI), or devoid of iron chelating properties, such as benzaldehyde isonicotinoyl hydrazone (BIH), and toxicity, oxidative stress and antioxidant (glutathione) metabolism were evaluated. Autoxidation of Fe(2+)in vitro increased in the presence of SIH or HAPI (but not BIH), an effect partially blocked by Fe(2+) chelation. Incubation of HepG2 cells with SIH or HAPI (but not BIH) was non-toxic and increased reactive oxygen species (ROS) levels, activated the transcription factor Nrf2, induced the catalytic subunit of γ-glutamate cysteine ligase (Gclc), and increased glutathione concentration. Fe(2+) chelation decreased ROS and inhibited Nrf2 activation, and Nrf2 knock-down inhibited the induction of Gclc in the presence of HAPI. Inhibition of γ-glutamate cysteine ligase enzymatic activity inhibited the increase in glutathione caused by HAPI, and increased oxidative stress. SIH iron chelators display both prooxidant (increasing the autoxidation rate of Fe(2+)) and antioxidant (activating Nrf2 signaling) effects. Activation by SIH iron chelators of a hormetic antioxidant response contributes to their antioxidant properties and modulates the anti- and pro-oxidant balance. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures

    Directory of Open Access Journals (Sweden)

    Eleonidas Moura Lima

    2005-07-01

    Full Text Available The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifter technique has the advantage of not requiring the use of some reagents for the obtention of metaphases and also of diminishing the consumption of maintenance reagents for this cell line.A linhagem celular Hep-2 é formada por células de carcinoma da laringe e é muito utilizada em modelos de carcinogênese e mutagenêse. Para avaliar o potencial proliferativo desta linhagem, desenvolvemos uma metodologia citogenética (técnica do sobrenadante para obtenção de metáfases a partir de células que, ao entrarem em mitose, perdem adesão celular, ficando em suspensão no meio de cultura. Através deste procedimento, foram contadas 2000 células, correspondendo a um índice mitótico (IM de 22.2% . Apesar de o IM obtido por esta técnica não ter sido estatisticamente diferente do IM obtido por outras metodologias citogenéticas clássicas, a técnica do sobrenadante é vantajosa porque elimina o uso de alguns reagentes utilizados na obtenção de metáfases e também diminui o consumo de reagentes de manutenção desta linhagem.

  20. IV Consenso Brasileiro para pesquisa de autoanticorpos em células HEp-2

    Directory of Open Access Journals (Sweden)

    Paulo Luiz Carvalho Francescantonio

    2014-01-01

    Full Text Available Objetivo: O IV Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2 (FAN realizado em Vitória (ES, no dia 18 de setembro de 2012, objetivou discutir estratégias e recomendações relacionadas ao procedimento técnico, à padronização e à interpretação dos resultados da pesquisa de autoanticorpos em células HEp-2. Métodos: Participaram do evento 23 pesquisadores e especialistas de Universidades e laboratórios brasileiros. Foram abordados diferentes tópicos, discutidos amplamente a fim de se estabelecer recomendações específicas. Resultados e conclusão: O IV Consenso integrou à árvore de decisão o padrão citoplasmático em Anéis e Bastões, o padrão nuclear pontilhado Quasi-homogêneo (QH e o padrão misto CENP-F. Discutiu-se ainda a necessidade de atenção para a classificação do padrão misto relacionado à presença de anticorpos anti-DNA topoisomerase I (Scl70, compreendendo os componentes nuclear pontilhado fino, nucleolar homogêneo, NOR na placa metafásica e citoplasmático pontilhado fino. Foram sugeridas diretrizes para o controle de qualidade do teste, diluição de triagem e diluição de esgotamento, e foi emitido alerta quanto à necessidade de atenção em relação à heterogeneidade de substratos disponíveis no mercado e a utilização de metodologias automatizadas para detecção de autoanticorpos.

  1. Metabolismo de fármacos y lesión hepática

    Directory of Open Access Journals (Sweden)

    Elso Manuel Cruz Cruz

    2015-03-01

    Full Text Available Los medicamentos han cambiado la forma de tratar las enfermedades y diferentes estados de salud alterados. Sin embargo, pese a todas las ventajas que ofrecen, cada vez existen más conocimientos acerca de las reacciones adversas que pueden causar. La aprobación de un fármaco para su comercialización no significa que su relación riesgo/beneficio está establecida de manera definitiva; indica que su eficacia ha sido demostrada y que los efectos adversos detectados hasta su aprobación son aceptables, con relación al tipo de afección al que se destina. El hígado es el principal responsable del metabolismo de los fármacos, nutrientes y otras sustancias. El consumo de medicamentos puede inducir daños en este órgano; la hepatotoxicidad provocada es causa de lesiones y hasta insuficiencia hepática aguda. (1 En la literatura se refiere que la hepatotoxicidad secundaria al consumo de fármacos tiene una incidencia relativamente alta, entre 1/10000 y 1/100000 medicamentos prescriptos, (2 aunque se reconoce que respecto a este tema existen subregistros que no permiten precisar la magnitud del problema. La inducción de una lesión hepática es una complicación potencial ante cualquier tratamiento farmacológico. Históricamente, este problema ha representado la principal causa de retiro del mercado, a escala mundial, de varios fármacos y es la reacción adversa causante del abandono en el desarrollo de nuevos medicamentos. En muestras representativas de pacientes con insuficiencia hepática aguda se ha encontrado algún vínculo con la ingestión de fármacos; muchos de estos pacientes requieren tratamiento adicional para el daño hepático, el cual puede llegar en casos extremos a necesitar el trasplante del órgano. (2 Existen dos tipos de reacciones relacionadas con la toxicidad hepática originada por medicamentos, intrínseca o idiosincrática. La intrínseca, debida a la dosis de los fármacos, es predecible, reproducible, y representa

  2. Summary of the third control system cyber-security (CS)2/HEP workshop

    International Nuclear Information System (INIS)

    Lueders, S.

    2012-01-01

    Over the last decade modern accelerator and experiment control systems have increasingly been based on commercial-off-the-shelf products (VME crates, programmable logic controllers (PLCs), supervisory control and data acquisition (SCADA) systems, etc.), on Windows or Linux PCs, and on communication infrastructures using Ethernet and TCP/IP. Despite the benefits coming with this (r)evolution, new vulnerabilities are inherited, too: Worms and viruses spread within seconds via the Ethernet cable, and attackers are becoming interested in control systems. The Stuxnet worm of 2010 against a particular Siemens PLC is a unique example for a sophisticated attack against control systems. Unfortunately, control PCs cannot be patched as fast as office PCs. Even worse, vulnerability scans at CERN using standard IT tools have shown that commercial automation systems lack fundamental security precautions: Some systems crashed during the scan, others could easily be stopped or their process data being altered. The third (CS)2/HEP workshop was intended to raise awareness; exchange good practices, ideas, and implementations; discuss what works and what not as well as their pros and cons; report on security events, lessons learned and successes; and update on progresses made at HEP laboratories around the world in order to secure control systems. It appears that deploying a 'Defense-in-depth approach is mandatory and corresponds to good practice while the full compliance to ISO-27000 standard is definitely both an ultimate goal and a very difficult challenge. There was a broad consensus to state that developing a 'security culture' among the players whatever they are: system experts, administrators, vendors or operators is the first step to do

  3. Molecular aspects of hepatic carcinogenesis Aspectos moleculares da carcinogênese hepática

    Directory of Open Access Journals (Sweden)

    Marcelo Eidi NITA

    2002-02-01

    Full Text Available Exogenous agents correlated with hepatocellular carcinoma (HCC have been identified and well characterized. These agents, including the different viruses that cause chronic hepatitis and cirrhosis, can lead to regenerative nodules and dysplastic nodules/adenomatous hyperplasia. These conditions associated with several molecular alterations of hepatocyte ultimately culminate in hepatocellular carcinoma. Recently, there has been a great progress in the identification of somatic and germinative mutations that may be correlated with the development of HCC, justifying a review on the subject. Hence, the factors involved in the process of hepatic carcinogenesis, such as infection by the hepatitis B and C viruses, with a special focus in the molecular alterations described in recent years are discussed herein, pointing out areas potentially relevant for clinical development.Agentes exógenos correlacionados com o carcinoma hepatocelular (HCC têm sido identificados e bem caracterizados. Esses agentes, entre os quais se incluem os diferentes vírus que causam hepatite e cirrose hepática, podem provocar o aparecimento de nódulos regenerativos e nódulos displásicos/hiperplasia adenomatosa. Essas condições associadas com diversas alterações moleculares do hepatócito podem culminar com o aparecimento do HCC. Recentemente, grandes progressos têm ocorrido na identificação de mutações somáticas ou germinativas que estariam correlacionadas com o desenvolvimento do HCC, justificando ampla revisão do tema. Procuramos discutir nesta revisão os fatores envolvidos no processo de carcinogênese hepática, tal como a infecção pelos vírus das hepatites B e C, com ênfase nas alterações moleculares descritas nos últimos anos, assinalando áreas em que potenciais avanços na abordagem clínica poderão surgir em futuro próximo.

  4. Inhibitory effects of Cassia tora L. on benzo[a]pyrene-mediated DNA damage toward HepG2 cells.

    Science.gov (United States)

    Wu, C H; Hsieh, C L; Song, T Y; Yen, G C

    2001-05-01

    The effects of water extracts from Cassia tora L. (WECT) treated with different degrees of roasting on benzo[a]pyrene (B[a]P)-induced DNA damage in human hepatoma cell line HepG2 were investigated via the comet assay without exogenous activation mixtures, such as S9 mix. WECT alone, at concentrations of 0.1-2 mg/mL, showed neither cytotoxic nor genotoxic effect toward HepG2 cells. B[a]P-induced DNA damage in HepG2 cells could be reduced by WECT in a dose-dependent manner (P roasted at 150 degrees C (60%) > roasted at 250 degrees C (23%). Ethoxyresorufin-O-dealkylase activity of HepG2 cells was effectively inhibited by WECT, and a similar trend of inhibition was observed in the order unroasted (64%) > roasted at 150 degrees C (42%) > roasted at 250 degrees C (18%). The activity of NADPH cytochrome P-450 reductase was also decreased by unroasted and 150 degrees C-roasted samples (50% and 38%, respectively). Furthermore, glutathione S-transferase activity was increased by treatment with unroasted (1.26-fold) and 150 degrees C-roasted (1.35-fold) samples at 1 mg/mL. In addition, the contents of anthraquinones (AQs) in WECT, including chrysophanol, emodin, and rhein, were decreased with increasing roasting temperature. Each of these AQs also demonstrated significant antigenotoxic activity in the comet assay. The inhibitory effects of chrysophanol, emodin, and rhein on B[a]P-mediated DNA damage in HepG2 cells were 78, 86, and 71%, respectively, at 100 microM. These findings suggested that the decreased antigenotoxicity of the roasted samples might be due to a reduction in their AQs content.

  5. Kaempferol induces apoptosis in HepG2 cells via activation of the endoplasmic reticulum stress pathway.

    Science.gov (United States)

    Guo, Haiqing; Ren, Feng; Zhang, Li; Zhang, Xiangying; Yang, Rongrong; Xie, Bangxiang; Li, Zhuo; Hu, Zhongjie; Duan, Zhongping; Zhang, Jing

    2016-03-01

    Kaempferol is a flavonoid compound that has gained importance due to its antitumor properties; however, the underlying mechanisms remain to be fully understood. The present study aimed to investigate the molecular mechanisms of the antitumor function of kaempferol in HepG2 hepatocellular carcinoma cells. Kaempferol was determined to reduce cell viability, increase lactate dehydrogenase activity and induce apoptosis in a concentration‑ and time‑dependent manner in HepG2 cells. Additionally, kaempferol‑induced apoptosis possibly acts via the endoplasmic reticulum (ER) stress pathway, due to the significant increase in the protein expression levels of glucose‑regulated protein 78, glucose‑regulated protein 94, protein kinase R‑like ER kinase, inositol‑requiring enzyme 1α, partial activating transcription factor 6 cleavage, caspase‑4, C/EBP homologous protein (CHOP) and cleaved caspase‑3. The pro‑apoptotic activity of kaempferol was determined to be due to induction of the ER stress‑CHOP pathway, as: i) ER stress was blocked by 4‑phenyl butyric acid (4‑PBA) pretreatment and knockdown of CHOP with small interfering RNA, which resulted in alleviation of kaempferol‑induced HepG2 cell apoptosis; and ii) transfection with plasmid overexpressing CHOP reversed the protective effect of 4‑PBA in kaempferol‑induced HepG2 cells and increased the apoptotic rate. Thus, kaempferol promoted HepG2 cell apoptosis via induction of the ER stress‑CHOP signaling pathway. These observations indicate that kaempferol may be used as a potential chemopreventive treatment strategy for patients with hepatocellular carcinoma.

  6. Galactosylated poly(ε-caprolactone) membrane promoted liver-specific functions of HepG2 cells in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yan, E-mail: zhang_yan@ecust.edu.cn [The Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237 (China); Zhang, Yi [The Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237 (China); Chen, Min; Zhou, Yan [The State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, Shanghai, 200237 (China); Lang, Meidong, E-mail: mdlang@ecust.edu.cn [The Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237 (China)

    2014-08-01

    The lack of pendant functional groups on the PCL backbone has been a great challenge for surface bioactivation of poly(ε-caprolactone) (PCL). In the present study, covalently galactosylated PCL (GPCL) was developed through coupling between the amino-functionalized PCL (NPCL) and the lactobionic acid (LA) and its potential application in maintenance of physiological functions of HepG2 cells was further evaluated. The structure and properties of GPCL were explored by {sup 1}H NMR, FT-IR, GPC and DSC. Moreover, the incorporation of galactose ligands onto GPCL membranes not only promoted higher wettability, but also radically changed surface morphology in comparison with PCL and NPCL according to the contact angle measurement and atomic force microscopy. When HepG2 cells were seeded onto these membranes, the cells on GPCL membranes showed more pronounced cell adhesion and tended to form aggregates during the initial adhesion stage and then progressively grew into multi-layer structures compared to those without galactose ligands by the observation with fluorescence microscope and scanning electron microscopy. Furthermore, live–dead assay and functional tests demonstrated that HepG2 cells on GPCL membranes had superior viability and maintained better liver-specific functions. Collectively, GPCL has great potential for hepatic tissue engineering scaffolds. - Graphical abstract: The specific recognition between the galactose ligands on the galactosylated poly(ε-caprolactone) membrane and the ASGPR on the HepG2 cell surface. The galactosylated poly(ε-caprolactone) membranes improved the cell-matrix interaction. The galactosylated functionalized PCL scaffold is a potential candidate for liver tissue engineering. - Highlights: • The specific recognition between the galactose ligands on the galactosylated poly(ε-caprolactone) membrane and the ASGPR on the HepG2 cell surface. • The galactosylated poly(ε-caprolactone) membranes improved the cell-matrix interaction.

  7. Autophagy suppresses proliferation of HepG2 cells via inhibiting glypican-3/wnt/β-catenin signaling

    Directory of Open Access Journals (Sweden)

    Hu P

    2018-01-01

    Full Text Available Pei Hu,1,2 Bin Cheng,3 Yulin He,3 Zhiqiang Wei,3 Dongfang Wu,1 Zhongji Meng3,4 1Department of Pharmacy, Zhongnan Hospital of Wuhan University, Wuhan, 2Department of Clinical Laboratory Medicine, 3Institute of Biomedical Research, 4Department of Infectious Disease, Taihe Hospital, Hubei University of Medicine, Shiyan, People’s Republic of China Introduction: Autophagy plays an important role in the growth and survival of hepatocellular carcinoma (HCC cells through several target proteins or signaling pathways. Glypican-3 (GPC3 is a new reliable HCC marker, which is involved in tumor growth in HCC, primarily mediated by wnt/β-catenin signaling. Objective: The present study aimed to identify the role of autophagy in the proliferation of HepG2 cells through GPC3/wnt/β-catenin signaling. Results and discussion: Results demonstrated that induction of autophagy by nutrition starvation and rapamycin treatment led to the downregulation of GPC3 expression in HepG2 cells, accompanied by the decreased expression of wnt downstream target genes (β-catenin, c-myc and cyclin D1. On the other hand, inhibition of autophagy by 3-methyl adenine (3-MA could rescue rapamycin-directed downregulation of GPC3 and wnt/β-catenin target genes and augment the proliferation of HepG2 cells. Furthermore, interference of GPC3 by siRNA suppressed wnt/β-catenin signaling and attenuated 3-MA stimulation of HepG2 cell proliferation. More interestingly, the mRNA of GPC3 remained unchanged when the protein levels of GPC3 were decreased by autophagy activation, suggesting that induction of autophagy may accelerate the degradation of GPC3. Conclusion: These results suggest that autophagy suppresses proliferation of HepG2 cells partially by inhibition of GPC3/wnt/β-catenin signaling. Keywords: hepatocellular carcinoma, glypican-3, autophagy, proliferation, wnt/β-catenin signaling

  8. Cytotoxic effect of Agaricus bisporus and Lactarius rufus β-D-glucans on HepG2 cells.

    Science.gov (United States)

    Pires, Amanda do Rocio Andrade; Ruthes, Andrea Caroline; Cadena, Silvia Maria Suter Correia; Acco, Alexandra; Gorin, Philip Albert James; Iacomini, Marcello

    2013-07-01

    The cytotoxic activity of β-D-glucans isolated from Agaricus bisporus and Lactarius rufus fruiting bodies was evaluated on human hepatocellular carcinoma cells (HepG2). NMR and methylation analysis suggest that these β-d-glucans were composed of a linear (1→6)-linked and a branched (1→3), (1→6)-linked backbone, respectively. They both decreased cell viability at concentrations of up to 100 μg mL(-1), as shown by MTT assay. The amount of LDH released and the analysis of cell morphology corroborated these values and also showed that the β-D-glucan of L. rufus was more cytotoxic to HepG2 cells than that of A. bisporus. The treatment of HepG2 cells with L. rufus and A. bisporus β-D-glucans at a dose of 200 μg mL(-1) for 24h promoted an increase of cytochrome c release and a decrease of ATP content, suggesting that these polysaccharides could promote cell death by apoptosis. Both β-D-glucans were tested against murine primary hepatocytes at a dose of 200 μg mL(-1). The results suggest that the L. rufus β-d-glucan was as cytotoxic for hepatocytes as for HepG2 cells, whereas the A. bisporus β-D-glucan, under the same conditions, was cytotoxic only for HepG2 cells, suggesting cell selectivity. These results open new possibilities for use of mushroom β-D-glucans in cancer therapy. Copyright © 2013 Elsevier B.V. All rights reserved.

  9. Ginseng (Panax quinquefolius and Licorice (Glycyrrhiza uralensis Root Extract Combinations Increase Hepatocarcinoma Cell (Hep-G2 Viability

    Directory of Open Access Journals (Sweden)

    David G. Popovich

    2011-01-01

    Full Text Available The combined cytoactive effects of American ginseng (Panax quinquefolius and licorice (Glycyrrhiza uralensis root extracts were investigated in a hepatocarcinoma cell line (Hep-G2. An isobolographic analysis was utilized to express the possibility of synergistic, additive or antagonistic interaction between the two extracts. Both ginseng and licorice roots are widely utilized in traditional Chinese medicine preparations to treat a variety of ailments. However, the effect of the herbs in combination is currently unknown in cultured Hep-G2 cells. Ginseng (GE and licorice (LE extracts were both able to reduce cell viability. The LC50 values, after 72 h, were found to be 0.64 ± 0.02 mg/mL (GE and 0.53 ± 0.02 mg/mL (LE. An isobologram was plotted, which included five theoretical LC50s calculated, based on the fixed fraction method of combination ginseng to licorice extracts to establish a line of additivity. All combinations of GE to LE (1/5, 1/3, 1/2, 2/3, 4/5 produced an effect on Hep-G2 cell viability but they were all found to be antagonistic. The LC50 of fractions 1/3, 1/2, 2/3 were 23%, 21% and 18% above the theoretical LC50. Lactate dehydrogenase release indicated that as the proportion of GE to LE increased beyond 50%, the influence on membrane permeability increased. Cell-cycle analysis showed a slight but significant arrest at the G1 phase of cell cycle for LE. Both GE and LE reduced Hep-G2 viability independently; however, the combinations of both extracts were found to have an antagonistic effect on cell viability and increased cultured Hep-G2 survival.

  10. Influence of Hepatitis B immunization to prevent vertical transmission of Hep-B virus in infants bam from Hep-B positive mother

    Directory of Open Access Journals (Sweden)

    Liza Fitria

    2016-10-01

    Full Text Available Background Hepatitis B is one of the most common infectious diseases worldv.ide. Indonesia has moderate-high endemicity for hepatitis B infection. Perinatal transmission increases the risk for chronic hepatitis B. Infants from HBsAg-positive mothers should receive hepatitis B immunoglobulin (HBIG and vaccination within 12 hours of birth, but this practice is not routinely done in Indonesia due to financial constraints. Objectives To detennine the influence of Hep-B immunization on preventing Hepatitis B vertical transmission. Methods A descriptive cohort study was conducted from May 2009 - January 2010. Subjects were term infants born from HBsAg-positive mothers v.ith no history of HBIG administration. They had received complete hepatitis B immunization and 1 month after the last dose were evaluated for HBsAg and anti-HBs. Cord blood was also taken during labor to measure HBsAg. Results There were 22 infants born from HBsAg-positive mother who met the inclusion criteria. HBsAgwas positive in 6 of22 cord blood specimens. There were 15 infants who completed this study. One of 15 infants had positive HBsAg after completed hepatitis B immunization and 12 of 15 infants had protective level of anti-HBs. Effectiveness of hepatitis B immunization to prevent vertical transmission in this study was 70-90%. Conclusion Hepatitis B immunization can prevent vertical transmission of hepatitis B in infants born to mothers who are HBsAg-positive even without administration of HBIG.

  11. Image, Image, Image

    Science.gov (United States)

    Howell, Robert T.

    2004-01-01

    With all the talk today about accountability, budget cuts, and the closing of programs in public education, teachers cannot overlook the importance of image in the field of industrial technology. It is very easy for administrators to cut ITE (industrial technology education) programs to save school money--money they might shift to teaching the…

  12. Habitat Evaluation Procedures (HEP) Report; Precious Lands Wildlife Management Area, Technical Report 2000-2003.

    Energy Technology Data Exchange (ETDEWEB)

    Kozusko, Shana

    2003-12-01

    The Nez Perce Tribe (NPT) currently manages a 15,325 acre parcel of land known as the Precious Lands Wildlife Management Area that was purchased as mitigation for losses incurred by construction of the four lower Snake River dams. The Management Area is located in northern Wallowa County, Oregon and southern Asotin County, Washington (Figure 1). It is divided into three management parcels--the Buford parcel is located on Buford Creek and straddles the WA-OR state line, and the Tamarack and Basin parcels are contiguous to each other and located between the Joseph Creek and Cottonwood Creek drainages in Wallowa County, OR. The project was developed under the Pacific Northwest Electric Power Planning and Conservation Act of 1980 (P.L. 96-501), with funding from the Bonneville Power Administration (BPA). The acreage protected under this contract will be credited to BPA as habitat permanently dedicated to wildlife and wildlife mitigation. A modeling strategy known as Habitat Evaluation Procedure (HEP) was developed by the U.S. Fish and Wildlife Service and adopted by BPA as a habitat equivalency accounting system. Nine wildlife species models were used to evaluate distinct cover type features and provide a measure of habitat quality. Models measure a wide range of life requisite variables for each species and monitor overall trends in vegetation community health and diversity. One product of HEP is an evaluation of habitat quality expressed in Habitat Units (HUs). This HU accounting system is used to determine the amount of credit BPA receives for mitigation lands. After construction of the four lower Snake River dams, a HEP loss assessment was conducted to determine how many Habitat Units were inundated behind the dams. Twelve target species were used in that evaluation: Canada goose, mallard, river otter, downy woodpecker, song sparrow, yellow warbler, marsh wren, western meadowlark, chukar, ring-necked pheasant, California quail, and mule deer. The U.S. Army Corp of

  13. "Contribuição da ressonância magnética na avaliação de doadores do lobo direito ao transplante hepático intervivos"

    OpenAIRE

    Gisele Warmbrand

    2004-01-01

    Este estudo teve, por finalidade, estabelecer o valor da ressonância magnética em 30 doadores potenciais do lobo direito do fígado, na determinação dos seguintes fatores: esteatose hepática; anatomia biliar; anatomias arterial hepática, venosas portal e hepática, e volume hepático lobar, comparando-os, respectivamente, com os achados anatomopatológicos da biópsia hepática, da colangiografia intraoperatória, da angiografia digital e/ou com os achados cirúrgicos, e com o peso real do enxerto. A...

  14. IMAGES, IMAGES, IMAGES

    Energy Technology Data Exchange (ETDEWEB)

    Marcus, A.

    1980-07-01

    The role of images of information (charts, diagrams, maps, and symbols) for effective presentation of facts and concepts is expanding dramatically because of advances in computer graphics technology, increasingly hetero-lingual, hetero-cultural world target populations of information providers, the urgent need to convey more efficiently vast amounts of information, the broadening population of (non-expert) computer users, the decrease of available time for reading texts and for decision making, and the general level of literacy. A coalition of visual performance experts, human engineering specialists, computer scientists, and graphic designers/artists is required to resolve human factors aspects of images of information. The need for, nature of, and benefits of interdisciplinary effort are discussed. The results of an interdisciplinary collaboration are demonstrated in a product for visualizing complex information about global energy interdependence. An invited panel will respond to the presentation.

  15. Daño hepático y enfermedad celíaca Liver damage and celiac disease

    Directory of Open Access Journals (Sweden)

    M. D. Cantarero Vallejo

    2007-11-01

    Full Text Available La enfermedad celiaca (EC es una causa importante de elevación de transaminasas: entre un 5 y un 10% de los pacientes con elevación crónica, criptogénica, de las transaminasas presentan EC y, al contrario, la EC puede estar asociada a diferentes enfermedades hepáticas. En efecto, un amplio abanico de patología hepática puede asociarse a EC, tanto en niños como en adultos, que pueden resumirse en: a daño hepático mínimo caracterizado por la ausencia de síntomas o signos clínicos asociables a una enfermedad hepática crónica y con cambios histológicos no específicos que desaparecen después de la introducción de una dieta sin gluten; b hepatopatías de etiología autoinmune, incluyendo la hepatitis autoinmune, la colangitis esclerosante primaria y la cirrosis biliar primaria, en las que la respectiva evolución no está influenciada por la introducción de dieta sin gluten; y c insuficiencia hepática grave y cirrosis hepática criptogénica descompensada, potencialmente tratables con la dieta sin gluten. Todas estas patologías están condicionadas por diferentes factores individuales y por una predisposición genética. La progresión y la reversibilidad del daño hepático en los diferentes cuadros patológicos, pueden estar condicionadas por la exposición al gluten y la edad, precoz o tardía, en la cual ha sido introducido en la dieta. Hay suficiente evidencia clínica para recomendar un atento cribado cruzado tanto para el diagnóstico del daño hepático asintomático en los pacientes con EC como para el diagnóstico de la EC en los pacientes con daño hepático criptogénico.Celiac disease (CD is an important cause of serum aminotransferase elevation: between 5 and 10% of patients with persistent and cryptogenetic transaminase elevation may have CD. In fact, a wide spectrum of liver injuries in children and adults may be related to CD, particularly: a mild parenchymal damage characterized by absence of any clinical signs

  16. Análisis del comportamiento de la procalcitonina en el paciente con cirrosis hepática ingresado en una unidad de cuidados intensivos

    OpenAIRE

    Villarreal Tello, Esther

    2012-01-01

    La procalcitonina (PCT) és un dels biomarcadors d'inflamació aplicats al camp de la infecció més estudiats en els últims anys. Es forma en diversos parènquimes incloent el fetge. Alguns autors van postular que la disfunció hepàtica podria ocasionar falsos negatius en la determinació de la procalcitonina. Estudiem la resposta de la PCT en pacients amb insuficiència hepàtica, analitzem retrospectivament el seu comportament en els pacients amb cirrosis hepàtica ingressats en la nostra unitat de ...

  17. Supervivencia en pacientes con trasplante hepático realizado en la Fundación Cardioinfantil entre 2005 y 2013

    OpenAIRE

    Olarte Parra, Camila; Otero Arrázola, Lina María

    2015-01-01

    Introducción: El trasplante hepático es la terapia de elección para los pacientes con enfermedad hepática terminal, logrando mejorar su expectativa y calidad de vida, de acuerdo a estudios realizados en otros países. En la Fundación Cardioinfantil – Instituto de Cardiología (FCI-IC) se han realizado 332 trasplantes hepáticos hasta el 2014, pero no se conoce la supervivencia y los factores pronósticos propios de los pacientes intervenidos. Objetivo Principal: Estimar la supervivencia a 1,...

  18. Finding Toxicological Tipping Points from High-Content Imaging Data (WC10)

    Science.gov (United States)

    A key challenge to using in vitro data in risk assessment is differentiating between chemical-induced adaptive versus adverse cellular responses. To further investigate this issue, we studied the effects of hundreds of chemicals in HepG2 cells using high-content imaging (HCI). HC...

  19. Habitat Evaluation Procedures (HEP) Report : Rainwater Wildlife Area, 1998-2001 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Childs, Allen

    2004-01-01

    The 8,768 acre Rainwater Wildlife Area was acquired in September 1998 by the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) through an agreement with Bonneville Power Administration (BPA) to partially offset habitat losses associated with construction of the John Day and McNary hydroelectric facilities on the mainstem Columbia River. U.S. Fish and Wildlife Service (USFWS) Habitat Evaluation Procedures (HEP) were used to determine the number of habitat units credited to BPA for acquired lands. Upland and riparian forest, upland and riparian shrub, and grassland rover types are evaluated in this study. Targeted wildlife species include downy woodpecker (Picoides pubescens), black-capped chickadee (Parus atricopillus), blue grouse (Dendragapus obscurus), great blue heron (Ardea herodias), yellow warbler (Dendroica petechia), mink (Mustela vison), and Western meadowlark (Sturnella neglects). Habitat surveys were conducted in 1998 and 1999 in accordance with published HEP protocols and included 65,300, 594m{sup 2} plots, and 112 one-tenth-acre plots. Between 153.3 and 7,187.46 acres were evaluated for each target wildlife mitigation species. Derived habitat suitability indices were multiplied by corresponding cover-type acreages to determine the number of habitat units for each species. The total baseline habitat units credited to BPA for the Rainwater Wildlife Area and its seven target species is 5,185.3 habitat units. Factors limiting habitat suitability are related to the direct, indirect, and cumulative effects of past livestock grazing, road construction, and timber harvest which have simplified the structure, composition, and diversity of native plant communities. Alternatives for protecting and improving habitat suitability include exclusion of livestock grazing, road de-commissioning/obliteration, reforestation and thinning, control of competing and unwanted vegetation (including noxious weeds), reestablishing displaced or reduced native

  20. Termoablação a laser de tumores hepáticos: atualização Laser-induced thermoablation of hepatic tumors: an update review

    Directory of Open Access Journals (Sweden)

    Giuseppe D'Ippolito

    2004-06-01

    Full Text Available A termoablação por raio laser de tumores hepáticos tem despontado como alternativa válida de tratamento em pacientes que não são candidatos a ressecção cirúrgica. O procedimento pode ser realizado por via percutânea, laparoscópica ou por laparotomia, e orientado por métodos de imagem. O objetivo deste trabalho é apresentar o mecanismo de ação deste método, bem como as suas indicações, contra-indicações, complicações e resultados clínicos, baseados em revisão bibliográfica.Laser-induced thermoablation has been used as a reliable method for producing coagulation necrosis in hepatic tumors in patients who are not suitable for surgical treatment. The procedure can be performed percutaneously, using image-guiding methods, by open laparotomy or laparoscopy. We review the current literature and discuss the principles, indications, complications and clinical results as well as the potential limitations and contraindications of this novel technique.

  1. Cyclosporine A and palmitic acid treatment synergistically induce cytotoxicity in HepG2 cells

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Yi, E-mail: yi.luo@pfizer.com; Rana, Payal; Will, Yvonne

    2012-06-01

    Immunosuppressant cyclosporine A (CsA) treatment can cause severe side effects. Patients taking immunosuppressant after organ transplantation often display hyperlipidemia and obesity. Elevated levels of free fatty acids have been linked to the etiology of metabolic syndromes, nonalcoholic fatty liver and steatohepatitis. The contribution of free fatty acids to CsA-induced toxicity is not known. In this study we explored the effect of palmitic acid on CsA-induced toxicity in HepG2 cells. CsA by itself at therapeutic exposure levels did not induce detectible cytotoxicity in HepG2 cells. Co-treatment of palmitic acid and CsA resulted in a dose dependent increase in cytotoxicity, suggesting that fatty acid could sensitize cells to CsA-induced cytotoxicity at the therapeutic doses of CsA. A synergized induction of caspase-3/7 activity was also observed, indicating that apoptosis may contribute to the cytotoxicity. We demonstrated that CsA reduced cellular oxygen consumption which was further exacerbated by palmitic acid, implicating that impaired mitochondrial respiration might be an underlying mechanism for the enhanced toxicity. Inhibition of c-Jun N-terminal kinase (JNK) attenuated palmitic acid and CsA induced toxicity, suggesting that JNK activation plays an important role in mediating the enhanced palmitic acid/CsA-induced toxicity. Our data suggest that elevated FFA levels, especially saturated FFA such as palmitic acid, may be predisposing factors for CsA toxicity, and patients with underlying diseases that would elevate free fatty acids may be susceptible to CsA-induced toxicity. Furthermore, hyperlipidemia/obesity resulting from immunosuppressive therapy may aggravate CsA-induced toxicity and worsen the outcome in transplant patients. -- Highlights: ► Palmitic acid and cyclosporine (CsA) synergistically increased cytotoxicity. ► The impairment of mitochondrial functions may contribute to the enhanced toxicity. ► Inhibition of JNK activity attenuated

  2. Dihydrotestosterone regulating apolipoprotein M expression mediates via protein kinase C in HepG2 cells

    Directory of Open Access Journals (Sweden)

    Yi-zhou Ye

    2012-12-01

    Full Text Available Abstract Background Administration of androgens decreases plasma concentrations of high-density lipid cholesterol (HDL-C. However, the mechanisms by which androgens mediate lipid metabolism remain unknown. This present study used HepG2 cell cultures and ovariectomized C57BL/6 J mice to determine whether apolipoprotein M (ApoM, a constituent of HDL, was affected by dihydrotestosterone (DHT. Methods HepG2 cells were cultured in the presence of either DHT, agonist of protein kinase C (PKC, phorbol-12-myristate-13-acetate (PMA, blocker of androgen receptor flutamide together with different concentrations of DHT, or DHT together with staurosporine at different concentrations for 24 hrs. Ovariectomized C57BL/6 J mice were treated with DHT or vehicle for 7d or 14d and the levels of plasma ApoM and livers ApoM mRNA were measured. The mRNA levels of ApoM, ApoAI were determined by real-time RT-PCR. ApoM and ApoAI were determined by western blotting analysis. Results Addition of DHT to cell culture medium selectively down-regulated ApoM mRNA expression and ApoM secretion in a dose-dependent manner. At 10 nM DHT, the ApoM mRNA levels were about 20% lower than in untreated cells and about 40% lower at 1000 nM DHT than in the control cells. The secretion of ApoM into the medium was reduced to a similar extent. The inhibitory effect of DHT on ApoM secretion was not blocked by the classical androgen receptor blocker flutamide but by an antagonist of PKC, Staurosporine. Agonist of PKC, PMA, also reduced ApoM. At 0.5 μM PMA, the ApoM mRNA levels and the secretion of ApoM into the medium were about 30% lower than in the control cells. The mRNA expression levels and secretion of another HDL-associated apolipoprotein AI (ApoAI were not affected by DHT. The levels of plasma ApoM and liver ApoM mRNA of DHT-treated C57BL/6 J mice were lower than those of vehicle-treated mice. Conclusions DHT directly and selectively down-regulated the level of ApoM mRNA and the

  3. Quantification of the uptake of silver nanoparticles and ions to HepG2 cells.

    Science.gov (United States)

    Yu, Su-juan; Chao, Jing-bo; Sun, Jia; Yin, Yong-guang; Liu, Jing-fu; Jiang, Gui-bin

    2013-04-02

    The toxic mechanism of silver nanoparticles (AgNPs) is still debating, partially because of the common co-occurrence and the lack of methods for separation of AgNPs and Ag(+) in biological matrices. For the first time, Triton-X 114-based cloud point extraction (CPE) was proposed to separate AgNPs and Ag(+) in the cell lysates of exposed HepG2 cells. Cell lysates were subjected to CPE after adding Na2S2O3, which facilitated the transfer of AgNPs into the nether Triton X-114-rich phase by salt effect and the preserve of Ag(+) in the upper aqueous phase through the formation of hydrophilic complex. Then the AgNP and Ag(+) contents in the exposed cells were determined by ICP-MS after microwave digestion of the two phases, respectively. Under the optimized conditions, over 67% of AgNPs in cell lysates were extracted into the Triton X-114-rich phase while 94% of Ag(+) remained in the aqueous phase, and the limits of detection for AgNPs and Ag(+) were 2.94 μg/L and 2.40 μg/L, respectively. This developed analytical method was applied to quantify the uptake of AgNPs to the HepG2 cells. After exposure to 10 mg/L AgNPs for 24 h, about 67.8 ng Ag were assimilated per 10(4) cells, in which about 10.3% silver existed as Ag(+). Compared to the pristine AgNPs (with 5.2% Ag(+)) for exposure, the higher ratio of Ag(+) to AgNPs in the exposed cells (10.3% Ag(+)) suggests the transformation of AgNPs into Ag(+) in the cells and/or the higher uptake rate of Ag(+) than that of AgNPs. Given that the toxicity of Ag(+) is much higher than that of AgNPs, the substantial content of Ag(+) in the exposed cells suggests that the contribution of Ag(+) should be taken into account in evaluating the toxicity of AgNPs to organisms, and previous results obtained by regarding the total Ag content in organisms as AgNPs should be reconsidered.

  4. A fully liquid DTPw-HepB-Hib combination vaccine for booster vaccination of toddlers in El Salvador Vacuna líquida combinada DTPw-HepB-Hib como dosis de refuerzo en infantes de El Salvador

    Directory of Open Access Journals (Sweden)

    Eduardo Suárez

    2010-02-01

    Full Text Available OBJECTIVES:To compare the safety and immunogenicity of a booster dose of a fully liquid diphtheria-tetanus-whole cell pertussis-hepatitis B-Haemophilus influenzae type b (DTPw-HepB-Hib vaccine to the separate administration of commercially available DTPw and Hib vaccines in healthy toddlers. METHODS:An open-label, randomized, parallel-group, Phase III study conducted at six centers in San Salvador, El Salvador, during February-June 2006. Toddlers (15-24 months of age were eligible to participate if they had received primary immunization at 2, 4, and 6 months of age with a commercial DTPw-HepB/Hib vaccine requiring reconstitution. Participants received either one booster dose of DTPw-HepB-Hib fully liquid vaccine or DTPw and Hib vaccines administered separately. Blood samples were taken immediately prior to and at 1 month post-vaccination. For a 5-day period following vaccination, solicited adverse events were collected in subject diaries and assessed. RESULTS:The combined DTPw-HepB-Hib fully liquid vaccine was non-inferior to the separately administered DTPw and Hib vaccines, in terms of seroprotection/seroconversion rates for all antigens evaluated. The combination vaccine elicited a strong booster response as demonstrated by a large increase in antibodies against all vaccine antigens. The geometric mean concentrations (GMCs of all antibodies in the DTPw-HepB-Hib group exceeded the seroprotection/seroconversion thresholds by very large margins, although for some antigens they were somewhat lower than the corresponding titers in the comparator group. With the combination vaccine, considerably fewer solicited local and systemic adverse events, such as fever and irritability, were reported than with the comparator vaccines. CONCLUSIONS:This study demonstrates that the fully liquid combined DTPw-HepB-Hib vaccine is highly immunogenic and has a favorable safety profile when given as a booster vaccination to toddlers who have received a primary

  5. An autonomic approach to configure HEP (High Energy Physics) experiments, applied to LHCb (Large Hadron Collider beauty)

    CERN Document Server

    Abadie, L; Charpentier, P

    2006-01-01

    Properly configuring an HEP (High Energy Phys ics) experiment becomes a more and more complex task as the number of electronics modules grows and technologies evolve quickly. Anticipating a fault in the software or in the hardware during the configuration or the data taking requires an adaptive and modular control system. The introduction of autonomic tools and data bases in the HEP world is quite recent and contributes to implement a more reliable system . The LHCb control system innovates as it has been built using autonomic tools. The main contribution of this PhD is the implementation of an autonomic 3-Tier architectur e to configure the LHCb experiment which is a huge network of devices of different types, and its integrat ion in the control system. This new type of autonomics architecture consists of: • A database layer. A relational Oracle databa se implemented using the Oracle technology contains the information...

  6. Integrating Visualization Applications, such as ParaView, into HEP Software Frameworks for In-situ Event Displays

    Energy Technology Data Exchange (ETDEWEB)

    Lyon, A. L. [Fermilab; Kowalkowski, J. B. [Fermilab; Jones, C. D. [Fermilab

    2017-11-22

    ParaView is a high performance visualization application not widely used in High Energy Physics (HEP). It is a long standing open source project led by Kitware and involves several Department of Energy (DOE) and Department of Defense (DOD) laboratories. Futhermore, it has been adopted by many DOE supercomputing centers and other sites. ParaView is unique in speed and efficiency by using state-of-the-art techniques developed by the academic visualization community that are often not found in applications written by the HEP community. In-situ visualization of events, where event details are visualized during processing/analysis, is a common task for experiment software frameworks. Kitware supplies Catalyst, a library that enables scientific software to serve visualization objects to client ParaView viewers yielding a real-time event display. Connecting ParaView to the Fermilab art framework will be described and the capabilities it brings discussed.

  7. Biópsia hepática por laparotomia paracostal em bovinos e búfalos

    OpenAIRE

    Minervino,Antonio Humberto Hamad; Barrêto Júnior,Raimundo Alves; Rodrigues,Frederico Augusto Mazzocca Lopes; Ferreira,Rodrigo Nogueira Fernandes; Saut,João Paulo Elsen; Queiroz,Genilson Fernandes; Reis,Leonardo Frasson dos; Ortolani,Enrico Lippi

    2009-01-01

    A técnica de biópsia hepática em ruminantes tem importante valor no diagnóstico clínico de doenças tóxicas e metabólicas, em especial nos desequilíbrios minerais. As técnicas mais comumente utilizadas restringem análises devido ao limitado volume de tecido obtido. No presente trabalho, avaliou-se o uso de uma técnica de biópsia hepática por laparotomia paracostal em bovinos e búfalos. Foram utilizados 10 bovinos e 10 búfalos hígidos. Os animais foram mantidos em estação, sedados com xilazina ...

  8. Integrating Visualization Applications, such as ParaView, into HEP Software Frameworks for In-situ Event Displays

    Science.gov (United States)

    Lyon, A. L.; Kowalkowski, J. B.; Jones, C. D.

    2017-10-01

    ParaView is a high performance visualization application not widely used in High Energy Physics (HEP). It is a long standing open source project led by Kitware and involves several Department of Energy (DOE) and Department of Defense (DOD) laboratories. Futhermore, it has been adopted by many DOE supercomputing centers and other sites. ParaView is unique in speed and efficiency by using state-of-the-art techniques developed by the academic visualization community that are often not found in applications written by the HEP community. In-situ visualization of events, where event details are visualized during processing/analysis, is a common task for experiment software frameworks. Kitware supplies Catalyst, a library that enables scientific software to serve visualization objects to client ParaView viewers yielding a real-time event display. Connecting ParaView to the Fermilab art framework will be described and the capabilities it brings discussed.

  9. FV peptide induces apoptosis in HEp 2 and HeLa cells: an insight into the mechanism of induction

    Directory of Open Access Journals (Sweden)

    Karthigayan S

    2006-01-01

    Full Text Available Abstract The present study is an attempt to evaluate the antiproliferative potential of peptide (7.6 kDa from lionfish (Pterios volitans venom on cultured HEp2 and HeLa cells. Different dose of purified peptide (1, 2 and 4 μg/ml at different time points (12, 24 and 36 hrs were tested for antiproliferative index of the peptide. Among them, 2 μg/ml at 24 hrs was found to effectively inhibit cancer cell growth in vitro and did not cause any adverse effect on normal human lymphocytes. Apoptosis was examined by propidium iodide staining, confirmed by the expression of caspase-8 and caspase-3, down regulation of Bcl-2 expression and DNA fragmentation in treated cells, when compared to untreated HEp2 and HeLa cells. Thus fish venom peptide was found to selectively induce apoptosis in cancer cell.

  10. Implications of altered glutathione metabolism in aspirin-induced oxidative stress and mitochondrial dysfunction in HepG2 cells.

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    Haider Raza

    Full Text Available We have previously reported that acetylsalicylic acid (aspirin, ASA induces cell cycle arrest, oxidative stress and mitochondrial dysfunction in HepG2 cells. In the present study, we have further elucidated that altered glutathione (GSH-redox metabolism in HepG2 cells play a critical role in ASA-induced cytotoxicity. Using selected doses and time point for ASA toxicity, we have demonstrated that when GSH synthesis is inhibited in HepG2 cells by buthionine sulfoximine (BSO, prior to ASA treatment, cytotoxicity of the drug is augmented. On the other hand, when GSH-depleted cells were treated with N-acetyl cysteine (NAC, cytotoxicity/apoptosis caused by ASA was attenuated with a significant recovery in oxidative stress, GSH homeostasis, DNA fragmentation and some of the mitochondrial functions. NAC treatment, however, had no significant effects on the drug-induced inhibition of mitochondrial aconitase activity and ATP synthesis in GSH-depleted cells. Our results have confirmed that aspirin increases apoptosis by increased reactive oxygen species production, loss of mitochondrial membrane potential and inhibition of mitochondrial respiratory functions. These effects were further amplified when GSH-depleted cells were treated with ASA. We have also shown that some of the effects of aspirin might be associated with reduced GSH homeostasis, as treatment of cells with NAC attenuated the effects of BSO and aspirin. Our results strongly suggest that GSH dependent redox homeostasis in HepG2 cells is critical in preserving mitochondrial functions and preventing oxidative stress associated complications caused by aspirin treatment.

  11. Implications of altered glutathione metabolism in aspirin-induced oxidative stress and mitochondrial dysfunction in HepG2 cells.

    Science.gov (United States)

    Raza, Haider; John, Annie

    2012-01-01

    We have previously reported that acetylsalicylic acid (aspirin, ASA) induces cell cycle arrest, oxidative stress and mitochondrial dysfunction in HepG2 cells. In the present study, we have further elucidated that altered glutathione (GSH)-redox metabolism in HepG2 cells play a critical role in ASA-induced cytotoxicity. Using selected doses and time point for ASA toxicity, we have demonstrated that when GSH synthesis is inhibited in HepG2 cells by buthionine sulfoximine (BSO), prior to ASA treatment, cytotoxicity of the drug is augmented. On the other hand, when GSH-depleted cells were treated with N-acetyl cysteine (NAC), cytotoxicity/apoptosis caused by ASA was attenuated with a significant recovery in oxidative stress, GSH homeostasis, DNA fragmentation and some of the mitochondrial functions. NAC treatment, however, had no significant effects on the drug-induced inhibition of mitochondrial aconitase activity and ATP synthesis in GSH-depleted cells. Our results have confirmed that aspirin increases apoptosis by increased reactive oxygen species production, loss of mitochondrial membrane potential and inhibition of mitochondrial respiratory functions. These effects were further amplified when GSH-depleted cells were treated with ASA. We have also shown that some of the effects of aspirin might be associated with reduced GSH homeostasis, as treatment of cells with NAC attenuated the effects of BSO and aspirin. Our results strongly suggest that GSH dependent redox homeostasis in HepG2 cells is critical in preserving mitochondrial functions and preventing oxidative stress associated complications caused by aspirin treatment.

  12. Report of the First International Consensus on Standardized Nomenclature of Antinuclear Antibody HEp-2 Cell Patterns (ICAP) 2014-2015

    OpenAIRE

    Chan, Edward K L; Damoiseaux, Jan; Carballo, Orlando Gabriel; Conrad, Karsten; de Melo Cruvinel, Wilson; Francescantonio, Paulo Luiz Carvalho; Fritzler, Marvin J.; Garcia-De La Torre, Ignacio; Herold, Manfred; Mimori, Tsuneyo; Satoh, Minoru; von Mühlen, Carlos A.; Andrade, Luis E C

    2015-01-01

    During the 12th International Workshop on Autoantibodies and Autoimmunity held in Sao Paulo, Brazil, on August 28, 2014, a full day session was devoted to establishing a consensus on the nomenclature of staining patterns observed in the antinuclear antibody (ANA) indirect immunofluorescence test on HEp-2 cells. The current report summarizes the collective agreements with input from the host Brazilian and international communities that represented research, clinical, and diagnostic service lab...

  13. HepG2 cells develop signs of riboflavin deficiency within four days of culture in riboflavin-deficient medium*

    OpenAIRE

    Werner, Ricarda; Manthey, Karoline C.; Griffin, Jacob B.; Zempleni, Janos

    2005-01-01

    Flavin mononucleotide and flavin adenine dinucleotide are essential coenzymes in redox reactions. For example, flavin adenine dinucleotide is a coenzyme for both glutathione reductase and enzymes that mediate the oxidative folding of secretory proteins. Here we investigated short-term effects of moderately riboflavin-deficient culture medium on flavin-related responses in HepG2 hepatocarcinoma cells. Cells were cultured in riboflavin-deficient (3.1 nmol/L) medium for up to six days; controls ...

  14. Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells

    Energy Technology Data Exchange (ETDEWEB)

    Morita, Akinori, E-mail: morita@tokushima-u.ac.jp [Department of Radiation Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553 (Japan); Department of Radiological Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8509 (Japan); Tanimoto, Keiji; Murakami, Tomoki; Morinaga, Takeshi [Department of Radiation Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553 (Japan); Hosoi, Yoshio, E-mail: hosoi@med.tohoku.ac.jp [Department of Radiation Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553 (Japan); Department of Radiation Biology, Graduate School of Medicine, Tohoku University, Sendai 980-8575 (Japan)

    2014-01-24

    Highlights: • Oxidative ATM activation can occur in the absence of nuclear DNA damage response. • The oxidized Hep G2 cells were subjected to subcellular fractionation. • The obtained results suggest that the ATM activation occurs in mitochondria. • ATM failed to respond to oxidative stress in mitochondria-depleted Hep G2 cells. • Mitochondria are required for the oxidative activation of ATM. - Abstract: Ataxia–telangiectasia mutated (ATM) is a serine/threonine protein kinase that plays a central role in DNA damage response (DDR). A recent study reported that oxidized ATM can be active in the absence of DDR. However, the issue of where ATM is activated by oxidative stress remains unclear. Regarding the localization of ATM, two possible locations, namely, mitochondria and peroxisomes are possible. We report herein that ATM can be activated when exposed to hydrogen peroxide without inducing nuclear DDR in Hep G2 cells, and the oxidized cells could be subjected to subcellular fractionation. The first detergent-based fractionation experiment revealed that active, phosphorylated ATM was located in the second fraction, which also contained both mitochondria and peroxisomes. An alternative fractionation method involving homogenization and differential centrifugation, which permits the light membrane fraction containing peroxisomes to be produced, but not mitochondria, revealed that the light membrane fraction contained only traces of ATM. In contrast, the heavy membrane fraction, which mainly contained mitochondrial components, was enriched in ATM and active ATM, suggesting that the oxidative activation of ATM occurs in mitochondria and not in peroxisomes. In Rho 0-Hep G2 cells, which lack mitochondrial DNA and functional mitochondria, ATM failed to respond to hydrogen peroxide, indicating that mitochondria are required for the oxidative activation of ATM. These findings strongly suggest that ATM can be activated in response to oxidative stress in mitochondria

  15. Fatores de risco e conduta nas complicações do trato biliar no transplante hepático

    Directory of Open Access Journals (Sweden)

    Alexandre Coutinho Teixeira de Freitas

    Full Text Available A reconstrução biliar é um dos pontos vulneráveis do transplante hepático apresentando incidência de complicações biliares, variando de 10 a 35%, nos diversos estudos da literatura. Esse trabalho tem por objetivo apresentar a experiência do nosso serviço em relação à incidência e ao manejo das complicações biliares no transplante de fígado. Foram incluídos no estudo 147 transplantes hepáticos ortotópicos, com idade média de 37,3 anos, correspondendo a 88 procedimentos em pacientes do sexo masculino e 59 do sexo feminino. Complicações biliares ocorreram em 27 transplantes (18,36% em 25 pacientes (dois retransplantes. A presença de rejeição celular e de complicações vasculares foi identificada como fator de risco para as complicações biliares. A idade, o sexo, a etiologia da cirrose e a técnica utilizada na reconstrução biliar não foram fatores de risco. No total, foram empregados 52 cursos terapêuticos: tratamento cirúrgico em 23 vezes; tratamento endoscópico em 15 vezes; retransplante em sete vezes; drenagem biliar transparieto-hepática em seis vezes e um paciente está em lista de espera para retransplante. Conclui-se deste estudo que as complicações biliares são freqüentes após o transplante hepático e que as vasculares e a rejeição celular são fatores de risco.

  16. The role of miR-145 in stem cell characteristics of human laryngeal squamous cell carcinoma Hep-2 cells.

    Science.gov (United States)

    Karatas, Omer Faruk; Suer, Ilknur; Yuceturk, Betul; Yilmaz, Mehmet; Hajiyev, Yusif; Creighton, Chad J; Ittmann, Michael; Ozen, Mustafa

    2016-03-01

    The cancer stem-like cells (CSLCs) are tumorigenic cells promoting initiation, progression, and spread of the tumor. Accumulating evidences suggested the presence of CSLCs in distinct tumors including laryngeal squamous cell carcinoma (LSCC). MicroRNAs have been proposed as significant regulators of carcinogenesis, and several of them have been demonstrated to have direct roles in survival of CSLCs. In this study, we aimed to explore the role of miR-145, which is downregulated in LSCC, on cancer stem cell potency of laryngeal cancer cells. We initially showed the downregulation of miR-145 expression in tumor tissue samples and in CD133-enriched CSLCs. Quantitative reverse-transcription PCR (qRT-PCR) analysis of miR-145-transfected Hep-2 cells demonstrated the inhibitory role of miR-145 on stem cell markers like SOX2, OCT4, KLF4, and ABCG2. We, then, investigated the stem cell features of miR-145-overexpressing Hep-2 cells by sphere formation assay, single-cell cloning assay, and aldehyde dehydrogenase (ALDH) assay, which all demonstrated the inhibition of stem cell potency upon miR-145 overexpression. Further qRT-PCR analysis demonstrated altered expression of epithelial to mesenchymal transition markers in miR-145-overexpressing Hep-2 cells. In conclusion, we demonstrated the regulatory role of miR-145 in stem cell characteristics of Hep-2 cells. Based on these results, we propose that miR-145 might carry crucial roles in LSCC tumorigenesis, prognosis, metastasis, chemoresistance, and recurrence through regulating stem cell properties of tumor cells.

  17. Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2 cell line

    Directory of Open Access Journals (Sweden)

    Nonpunya Apiyada

    2011-10-01

    Full Text Available Abstract Background Six plants from Thailand were evaluated for their cytotoxicity and apoptosis induction in human hepatocarcinoma (HepG2 as compared to normal African green monkey kidney epithelial cell lines. Methods Ethanol-water crude extracts of the six plants were tested with neutral red assay for their cytotoxicity after 24 hours of exposure to the cells. Apoptotic induction was tested in the HepG2 cells with diamidino-2-phenylindole staining. DNA fragmentation, indicative of apoptosis, was analyzed with agarose gel electrophoresis. Alkylation, indicative of DNA damage, was also evaluated in vitro by 4-(4'-nitrobenzyl pyridine assay. Results The extract of Pinus kesiya showed the highest selectivity (selectivity index = 9.6 and potent cytotoxicity in the HepG2 cell line, with an IC50 value of 52.0 ± 5.8 μg/ml (mean ± standard deviation. Extract of Catimbium speciosum exerted cytotoxicity with an IC50 value of 55.7 ± 8.1 μg/ml. Crude extracts from Glochidion daltonii, Cladogynos orientalis, Acorus tatarinowii and Amomum villosum exhibited cytotoxicity with IC50 values ranging 100-500 μg/ml. All crude extracts showed different alkylating abilities in vitro. Extracts of P. kesiya, C. speciosum and C. orientalis caused nuclei morphological changes and DNA laddering. Conclusion The extracts of C. speciosum, C. orientalis and P. kesiya induced apoptosis. Among the three plants, P. kesiya possessed the most robust anticancer activity, with specific selectivity against HepG2 cells.

  18. Heat Killed Attenuated Leishmania Induces Apoptosis of HepG2 Cells Through ROS Mediated p53 Dependent Mitochondrial Pathway

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    Dipayan Bose

    2016-03-01

    Full Text Available Background/Aims: Cytotoxic effect of attenuated Leishmania on liver cancer cells by inducing ROS generation. Methods: Spectrophotometric study to analyze cell death and levels of different active caspases. Flow cytometric study was done to analyze apoptosis induction and ROS generation and levels of different protein. Western blot analysis was performed to study the levels of protein. Confocal microscopy was done to ascertain the expression of different apoptotic markers. Results: We have now observed that attenuated Leishmania donovani UR6 also has potentiality towards growth inhibition of HepG2 cells and investigated the mechanism of action. The effect is associated with increased DNA fragmentation, rise in number of annexinV positive cells, and cell cycle arrest at G1 phase. The detection of unregulated levels of active PARP, cleaved caspases 3 and 9, cytosolic cytochrome C, Bax, and Bad, along with the observed downregulation of Bcl-2 and loss of mitochondrial membrane potential suggested the involvement of mitochondrial pathway. Enhanced ROS and p53 levels regulate the apoptosis of HepG2 cells. NAC was found to inhibit p53 production but PFT-α has no effect on ROS generation. In conclusion, Leishmania donovani UR6 efficiently induces apoptosis in HepG2 cells through ROS mediated p53 dependent mitochondrial pathway. Conclusion: It has been reported earlier that some parasites show prominent cytotoxic effect and prevent tumor growth. From our study we found that Leishmania donovani UR6 efficiently induced apoptosis in HepG2 cells through ROS mediated p53 dependent mitochondrial pathway. This study has rejuvenated the age old idea of bio-therapy.

  19. [Effect of extracted ZG from gardenia on Hep-2 cell membrane post infected with parainfluenza virus type 1 (PIV-1)].

    Science.gov (United States)

    Guo, Shan-Shan; Huang, Yang; Zhao, Ye; Gao, Ying-Jie; Gong, Wen-Feng; Cui, Xiao-Lan

    2007-09-01

    In order to study the anti-viral mechanism of extracted ZG from Gardenia, the effect of extracted ZG on Hep-2 cell membrane potential, Na -K+-ATPase activity and membrane fluidity post infected with parainfluenza virus type 1 (PIV-1) was observed. Acetylcholine which was fluorescent labeled with DiBAC4 (3) was taken as positive control to observe the changes of membrane potential and was measured by flow cytometer. The phosphorus determination method and spectrophotometer were used to measure the Na+-K+-ATPase activity of Hep-2 cell membrane post PIV-1 infection. Hep-2 cell membrane phospholipids was labeled with fluorescent NBD-C6-HPC and membrane fluidity was measured by confocal laser scanning microscope. The results demonstated that after PIV-1 infection the Hep-2 cell membrane potential decreased significantly and the membrane was in the state of hyperpolarization, Na+-K+-ATPase activity increased and membrane fluidity decreased significantly. There was no apparent interferring effect of extracted ZG on the changes of membrane potential and Na+-K+-ATPase activity post PIV-1 infection, while membrane fluidity was improved significantly. Acetylcholine improved the state of hyperpolarization. The changes of membrane potential, Na -K+-ATPase activity and membrane fluidity might be the biomechanism of PIV-1 infectoin. The extracted ZG improved membrane fluidity to prevent from PIV-1 infection by protecting the cell membrane, which was probably the mechanism of anti-PIV-1 activity of the extracted ZG, but ZG probably had nothing to do with membrane potential and Na+-K+-ATPase activity.

  20. LXR agonist increases apoE secretion from HepG2 spheroid, together with an increased production of VLDL and apoE-rich large HDL

    Directory of Open Access Journals (Sweden)

    Koike Kazuhiko

    2011-08-01

    Full Text Available Abstract Background The physiological regulation of hepatic apoE gene has not been clarified, although the expression of apoE in adipocytes and macrophages has been known to be regulated by LXR. Methods and Results We investigated the effect of TO901317, a LXR agonist, on hepatic apoE production utilizing HepG2 cells cultured in spheroid form, known to be more differentiated than HepG2 cells in monolayer culture. Spheroid HepG2 cells were prepared in alginate-beads. The secretions of albumin, apoE and apoA-I from spheroid HepG2 cells were significantly increased compared to those from monolayer HepG2 cells, and these increases were accompanied by increased mRNA levels of apoE and apoA-I. Several nuclear receptors including LXRα also became abundant in nuclear fractions in spheroid HepG2 cells. Treatment with TO901317 significantly increased apoE protein secretion from spheroid HepG2 cells, which was also associated with the increased expression of apoE mRNA. Separation of the media with FPLC revealed that the production of apoE-rich large HDL particles were enhanced even at low concentration of TO901317, and at higher concentration of TO901317, production of VLDL particles increased as well. Conclusions LXR activation enhanced the expression of hepatic apoE, together with the alteration of lipoprotein particles produced from the differentiated hepatocyte-derived cells. HepG2 spheroids might serve as a good model of well-differentiated human hepatocytes for future investigations of hepatic lipid metabolism.

  1. Comportamento da musculatura das arteríolas intra-hepáticas na forma hepatesplênica da esquistossomose mansônica

    Directory of Open Access Journals (Sweden)

    Maria do Socorro Almeida Barbosa

    1988-03-01

    Full Text Available A determinação da relação parede-lume das arteríolas intra-hepáticas, na esquistossomose mansônica (forma hepatesplênica, demonstra a existência de uma hipotrofia da camada muscular daqueles vasos. Esse achado sugere redução do fluxo sangüíneo arterial hepático nessa entidade.

  2. Comparison of the 3He(p,2p)d and 3He(p,pd)p reactions

    International Nuclear Information System (INIS)

    Bracco, A.; Gubler, H.P.; Hasell, D.K.; Oers, W.T.H. van; Epstein, M.B.; Krause, D.A.; Margaziotis, D.J.; Abegg, R.; Miller, C.A.

    1984-01-01

    In the present experiment the 3 He(p, 2p)d reaction was measured at 300 MeV at the angle pair 70 0 -33 0 and at 450 MeV at the angle pairs 70 0 -30 0 and 46 0 -46 0 for recoil momenta larger than 350 MeV/c. For the 3 He(p, pd)p reaction energy sharing loci were measured at 450 MeV for the angle pairs 25 0 -68 0 and 50 0 -68 0 yielding a recoil momentum range from 0 to 480 MeV/c. In the framework of the PWIA the proton-deuteron relative motion momentum distributions extracted from 3 He(p, S2p)d and 3 He(p,pd)p should be identical. A comparison of the two reactions is therefore a useful tool to investigate the presence of competing processes such as multiple scattering, final state interaction and isobar configuration effects. (orig.)

  3. In vitro investigations of Cynara scolymus L. extract on cell physiology of HepG2 liver cells

    Directory of Open Access Journals (Sweden)

    Gesine Löhr

    2009-06-01

    Full Text Available The objective of this study was the investigation of a potential influence of artichoke leaf extract (ALE on the cell physiology and gene expression of phase I/II enzymes of human liver cells HepG2 and investigation on potential cell protective effects against ethanol-induced cell toxicity against HepG2 cells. Cell biological assays under in vitro conditions using HepG2 liver cells and investigation of mitochondrial activity (MTT test, proliferation assay (BrdU incorporation ELISA, LDH as toxicity marker, gene expression analysis by RT-PCR and enzyme activity of glutationtransferase. Artichocke extract, containing 27% caffeoylquinic acids and 7% flavonoids induced mitochondrial activity, proliferation and total protein content under in vitro conditions in human liver cells HepG2. These effects could not be correlated to the well-known artichoke secondary compounds cynarin, caffeic acid, chlorogenic acid, luteolin and luteolin-7-O-glucoside. The flavones luteolin and luteolin-7-O-glucoside had inhibitory effects at 100 µg/mL level on HepG2 cells, with luteolin being a significant stronger inhibitor compared to the respective glucoside. Artichoke leaf extract had minor stimulating effect on gene expression of CYP1A2, while CYP3A4, GGT, GPX2, GSR and GST were slightly inhibited. GST inhibition under in vitro conditions was also shown by quantification of GST enzyme activity. Induction of gene expression of CYP1A2 was shown to be supraadditive after simultaneous application of ethanol plus artichoke extract. Artichoke leaf extract exhibited cell protective effects against ethanol-induced toxicity within cotreatment under in vitro conditions. Also H2O2 damage was significantly inhibited by simultaneous artichoke incubation. Pre- and posttreatments did not exert protective effects. DMSO-induced toxicity was significantly reduced by pre-, post- and cotreatment with artichoke extract and especially with luteolin-7-O-glucoside, indicating a direct

  4. An integrated approach to improved toxicity prediction for the safety assessment during preclinical drug development using Hep G2 cells

    International Nuclear Information System (INIS)

    Noor, Fozia; Niklas, Jens; Mueller-Vieira, Ursula; Heinzle, Elmar

    2009-01-01

    Efficient and accurate safety assessment of compounds is extremely important in the preclinical development of drugs especially when hepatotoxicty is in question. Multiparameter and time resolved assays are expected to greatly improve the prediction of toxicity by assessing complex mechanisms of toxicity. An integrated approach is presented in which Hep G2 cells and primary rat hepatocytes are compared in frequently used cytotoxicity assays for parent compound toxicity. The interassay variability was determined. The cytotoxicity assays were also compared with a reliable alternative time resolved respirometric assay. The set of training compounds consisted of well known hepatotoxins; amiodarone, carbamazepine, clozapine, diclofenac, tacrine, troglitazone and verapamil. The sensitivity of both cell systems in each tested assay was determined. Results show that careful selection of assay parameters and inclusion of a kinetic time resolved assay improves prediction for non-metabolism mediated toxicity using Hep G2 cells as indicated by a sensitivity ratio of 1. The drugs with EC 50 values 100 μM or lower were considered toxic. The difference in the sensitivity of the two cell systems to carbamazepine which causes toxicity via reactive metabolites emphasizes the importance of human cell based in-vitro assays. Using the described system, primary rat hepatocytes do not offer advantage over the Hep G2 cells in parent compound toxicity evaluation. Moreover, respiration method is non invasive, highly sensitive and allows following the time course of toxicity. Respiration assay could serve as early indicator of changes that subsequently lead to toxicity.

  5. Protective role of metabolism by intestinal microflora in butyl paraben-induced toxicity in HepG2 cell cultures.

    Science.gov (United States)

    Khanal, Tilak; Kim, Hyung Gyun; Jin, Sun Woo; Shim, Eol; Han, Hwa Jeong; Noh, Keumhan; Park, Sunkyoung; Lee, Dae Hun; Kang, Wonku; Yeo, Hee Kyung; Kim, Dong Hyun; Jeong, Tae Cheon; Jeong, Hye Gwang

    2012-09-03

    Parabens are alkyl esters of p-hydroxybenzoic acid (BA), including methyl paraben (MP), ethyl paraben, propyl paraben (PP), and butyl paraben (BP). In the present study, possible role of metabolism by fecalase in BP-induced cytotoxicity was investigated in HepG2 cell cultures. As an intestinal bacterial metabolic system, a human fecalase prepared from human fecal specimen was employed. Among the parabens tested, cytotoxicity of BP was most severe. BA, the de-esterified metabolite, did not induce cytotoxicity when compared to other parabens. When BP was incubated with fecalase, it rapidly disappeared, in association with reduced cytotoxicity in HepG2 cells. In addition, BP incubated with fecalase significantly caused an increase in Bcl-2 expression together with a decrease in Bax expression and cleaved caspase-3. Moreover, anti-apoptotic effect by the incubation of BP with fecalase was also confirmed by the TUNEL assay. Furthermore, BP induced a sustained activation of the phosphorylation of JNK only when it was treated alone. Meanwhile, BP-induced cell death was reversed by the pre-incubation of BP with either fecalase or SP600125. Taken together, the findings suggested that metabolism of BP by human fecalase might have protective effects against BP-induced toxicity in HepG2 cells. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  6. Rosemary Extracts Upregulate Nrf2, Sestrin2, and MRP2 Protein Level in Human Hepatoma HepG2 Cells

    Directory of Open Access Journals (Sweden)

    Xiao-pei Tong

    2017-01-01

    Full Text Available In the past few decades, the incidence of liver cancer has been rapidly rising across the world. Rosemary is known to possess antioxidant activity and is used as natural antioxidant food preservative. It is proposed to have anticancer activity in treating different tumor models. In this study, we try to explore the impact of rosemary extracts on upregulating the level of Nrf2 and Nrf2-regulatory proteins, Sestrin2 and MRP2 in HepG2 cells, and to speculate its potential mechanism. The anticancer activity of rosemary extract, including its polyphenolic diterpenes carnosic acid and carnosol, was evaluated to understand the potential effect on HepG2 cells. Rosemary extract, carnosic acid, and carnosol induced the expression of Sestrin2 and MRP2 associate with enhancement of Nrf2 protein level in HepG2 cells, in which carnosic acid showed most obvious effect. Although the activation pathway of Nrf2/ARE was not exactly assessed, it can be assumed that the enhancement of expression of Sestrin2 and MRP2 may result from upregulation of Nrf2.

  7. Dryocrassin ABBA Induces Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells Through a Caspase-Dependent Mitochondrial Pathway.

    Science.gov (United States)

    Jin, Zhe; Wang, Wen-Fei; Huang, Jian-Ping; Wang, He-Meng; Ju, Han-Xun; Chang, Ying

    2016-01-01

    Biological and pharmacological activities of dryocrassin ABBA, a phloroglucinol derivative extracted from Dryopteris crassirhizoma, have attracted attention. In this study, the apoptotic effect of dryocrassin ABBA on human hepatocellular carcinoma HepG2 cells was investigated. We tested the effects of dryocrassin ABBA on HepG2 in vitro by MTT, flow cytometry, real-time PCR, and Western blotting. KM male mice were used to detect the effect of dryocrassin ABBA on H22 cells in vivo. Dryocrassin ABBA inhibited the growth of HepG2 cells in a concentration-dependent manner. After treatment with 25, 50, and 75 μg/mL dryocrassin ABBA, the cell viability was 68%, 60% and 49%, respectively. Dryocrassin ABBA was able to induce apoptosis, measured by propidium iodide (PI)/annexin V-FITC double staining. The results of real-time PCR and Western ting showed that dryocrassin ABBA up-regulated p53 and Bax expression and inhibited Bcl-2 expression which led to an activation of caspase-3 and caspase-7 in the cytosol, and then induction of cell apoptosis. In vivo experiments also showed that dryocrassin ABBA treatment significantly suppressed tumor growth, without major side effects. Overall, these findings provide evidence that dryocrassin ABBA may induce apoptosis in human hepatocellular carcinoma cells through a caspase-mediated mitochondrial pathway.

  8. Time- and concentration-dependent effects of resveratrol in HL-60 and HepG2 cells

    DEFF Research Database (Denmark)

    Stervbo, Ulrik; Vang, Ole; Bonnesen, Christine

    2006-01-01

    Resveratrol, a phytochemical present in grapes, has been demonstrated to inhibit tumourigenesis in animal models. However, the specific mechanism by which resveratrol exerts its anticarcinogenic effect has yet to be elucidated. In the present study, the inhibitory effects of resveratrol on cell...... proliferation and apoptosis were evaluated in the human leukaemia cell line HL-60 and the human hepatoma derived cell line HepG2. We found that after a 2 h incubation period, resveratrol inhibited DNA synthesis in a concentration-dependent manner. The IC50 value was 15 μM in both HL-60 and HepG2 cells. When...... the time of treatment was extended, an increase in IC50 value was observed; for example, at 24 h the IC50 value was 30 μM for HL-60 cells and 60 μM for HepG2 cells. Flow cytometry revealed that cells accumulated in different phases of the cell cycle depending on the resveratrol concentration. Furthermore...

  9. Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells

    Directory of Open Access Journals (Sweden)

    Kin Weng Kong

    2016-01-01

    Full Text Available Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE and stem (BSE from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80% at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage.

  10. Development of On-chip Coculture System for Cytotoxicity Test Using Caco-2 and Hep G2

    Science.gov (United States)

    Kimura, Hiroshi; Nakayama, Hidenari; Yamamoto, Takatoki; Sakai, Yasuyuki; Fujii, Teruo

    We developed a chip-based coculture system for cytotoxicity test, as our continuous effort to develop a multi-functional micro culture device realized by integration of fluidic control. The culture zone in the device was divided into two compartments separated by a microporous membrane through which substances in culture medium can freely come-and-go to induce the mutual interactions between the cells cultured at each compartment. In this work, it was examined that 1) coculture and 2) cytotoxicity model through oral intake, using Caco-2 and Hep G2 cell as a model cell of small intestine and liver respectively. As a result of test 1), Hep G2 cells cocultured with Caco-2 show same albumin secretion activity as the one not cocultured with Caco-2 cells. As a result of test 2), The cytotoxicity of caffeine and paraquat on Hep G2 cells was successfully measured with and without association of a selective chemical barrier function of Caco-2 cells.

  11. Bioavailability and metabolism of rosemary infusion polyphenols using Caco-2 and HepG2 cell model systems.

    Science.gov (United States)

    Achour, Mariem; Saguem, Saad; Sarriá, Beatriz; Bravo, Laura; Mateos, Raquel

    2018-01-12

    Rosmarinus officinalis is an aromatic plant used in folk medicine as a result of the therapeutic properties associated with its phenolic composition, being rich in rosmarinic acid (RA) and caffeic acid (CA). To better understand the bioactivity of these compounds, their absorption and metabolism were assessed in human Caco-2 and HepG2 cells, as small intestine and liver models, respectively, using RA and CA standards, as well as a rosemary infusion and ferulic acid (FA). Test compounds were partially up-taken and metabolized by Caco-2 and HepG2 cells, although a higher metabolization rate was observed after hepatic incubation compared to intestinal incubation. CA was the compound best absorbed followed by RA and FA, showing metabolites percentages of 30.4%, 11.8% and 4.4% in Caco-2 and 34.3%, 10.3% and 3.2% in HepG2 cells, respectively. RA in the rosemary infusion showed improved bioavailability compared to pure RA. Methyl derivatives were the main metabolites detected for CA and RA after intestinal and hepatic metabolism, followed by methyl-glucuronidates and glucuronidates. RA was also minimally hydrolyzed into CA, whereas FA only was glucuronidated. Rosemary polyphenols followed the same biotransformation pathways as the standards. In addition, phase II derivatives of luteolin were observed. Rosemary polyphenols are partially metabolized in both the intestine and liver. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.

  12. Oroxylin A induced apoptosis of human hepatocellular carcinoma cell line HepG2 was involved in its antitumor activity

    International Nuclear Information System (INIS)

    Hu Yang; Yang Yong; You Qidong; Liu Wei; Gu Hongyan; Zhao Li; Zhang Kun; Wang Wei; Wang Xiaotang; Guo Qinglong

    2006-01-01

    We previously reported that wogonin, a flavonoid compound, was a potent apoptosis inducer of human hepatoma SMMC-7721 cells and murine sarcoma S180 cells. In the present study, the effect of oroxylin A, one wogonin structurally related flavonoid isolated from Scutellariae radix, on human hepatocellular carcinoma cell line HepG2 was examined and molecular mechanisms were also investigated. Oroxylin A inhibited HepG2 cell proliferation in a concentration- and time-dependent manner measured by MTT-assay. Treatment with an apoptosis-inducing concentration of oroxylin A caused typical morphological changes and apoptotic blebbing in HepG2 cells. DNA fragmentation assay was used to examine later apoptosis induced by oroxylin A. FACScan analysis revealed a dramatic increase in the number of apoptotic and G 2 /M phase arrest cells after oroxylin A treatment. The pro-apoptotic activity of oroxylin A was attributed to its ability to modulate the concerted expression of Bcl-2, Bax, and pro-caspase-3 proteins. The expression of Bcl-2 protein and pro-caspase-3 protein was dramatically decreased after treatment with oroxylin A. These results demonstrated that oroxylin A could effectively induce programmed cell death and suggested that it could be a promising antitumor drug

  13. Ascariasis hepática: reporte de un caso y revisión de la literatura

    Directory of Open Access Journals (Sweden)

    Martha Tuñón Pitalua

    2014-01-01

    Full Text Available El absceso hepático causado por áscaris es una presentación muy rara; constituye el 1 % de todos los casos y puede estar compuesto por huevos y/o gusanos adultos. Se presenta caso de mujer de 70 años, admitida por urgencias, con dolor tipo cólico en hemiabdomen superior, vómito, fiebre y sensación de masa; trae reporte de ecografía con colelitiasis, áscaris en el colédoco y aumento del tamaño pancreático. La TAC muestra imagen con aspecto de colección en hígado y parte anterior del páncreas. La biopsia hepática reporta infiltrado inflamatorio crónico mixto de predominio eosinofílico con células gigantes multinucleadas y necrosis, asociado a estructuras circulares compatible con ascariasis. Se realiza tratamiento con antibióticos y antihelmínticos, con evolución satisfactoria. En la literatura consultada son raros los casos reportados de ascariasis hepática, por lo cual es pertinente el reporte del caso.

  14. Biphasic Estradiol-induced AKT Phosphorylation Is Modulated by PTEN via MAP Kinase in HepG2 Cells

    Science.gov (United States)

    Marino, Maria; Acconcia, Filippo; Trentalance, Anna

    2003-01-01

    We reported previously in HepG2 cells that estradiol induces cell cycle progression throughout the G1–S transition by the parallel stimulation of both PKC-α and ERK signaling molecules. The analysis of the cyclin D1 gene expression showed that only the MAP kinase pathway was involved. Here, the presence of rapid/nongenomic, estradiol-regulated, PI3K/AKT signal transduction pathway, its modulation by the levels of the tumor suppressor PTEN, its cross-talk with the ERK pathway, and its involvement in DNA synthesis and cyclin D1 gene promoter activity have all been studied in HepG2 cells. 17β-Estradiol induced the rapid and biphasic phosphorylation of AKT. These phosphorylations were independent of each other, being the first wave of activation independent of the estrogen receptor (ER), whereas the second was dependent on ER. Both activations were dependent on PI3K activity; furthermore, the ERK pathway modulated AKT phosphorylation by acting on the PTEN levels. The results showed that the PI3K pathway, as well as ER, were strongly involved in both G1–S progression and cyclin D1 promoter activity by acting on its proximal region (-254 base pairs). These data indicate that in HepG2 cells, different rapid/nongenomic estradiol-induced signal transduction pathways modulate the multiple steps of G1–S phase transition. PMID:12808053

  15. Cocoa flavonoids improve insulin signalling and modulate glucose production via AKT and AMPK in HepG2 cells.

    Science.gov (United States)

    Cordero-Herrera, Isabel; Martín, María Angeles; Bravo, Laura; Goya, Luis; Ramos, Sonia

    2013-06-01

    Cocoa and (-)-epicatechin (EC), a main cocoa flavanol, have been suggested to exert beneficial effects in diabetes, but the mechanism for their insulin-like effects remains unknown. In this study, the modulation of insulin signalling by EC and a cocoa phenolic extract (CPE) on hepatic HepG2 cells was investigated by analysing key proteins of the insulin pathways, namely insulin receptor, insulin receptor substrate (IRS) 1 and 2, PI3K/AKT and 5'-AMP-activated protein kinase (AMPK), as well as the levels of the glucose transporter GLUT-2 and the hepatic glucose production. EC and CPE enhanced the tyrosine phosphorylation and total insulin receptor, IRS-1 and IRS-2 levels and activated the PI3K/AKT pathway and AMPK in HepG2 cells. CPE also enhanced the levels of GLUT-2. Interestingly, EC and CPE modulated the expression of phosphoenolpyruvate carboxykinase, a key protein involved in the gluconeogenesis, leading to a diminished glucose production. In addition, EC- and CPE-regulated hepatic gluconeogenesis was prevented by the blockage of AKT and AMPK. Our data suggest that EC and CPE strengthen the insulin signalling by activating key proteins of that pathway and regulating glucose production through AKT and AMPK modulation in HepG2 cells. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Expression of CAR in SW480 and HepG2 cells during G1 is associated with cell proliferation

    International Nuclear Information System (INIS)

    Osabe, Makoto; Sugatani, Junko; Takemura, Akiko; Yamazaki, Yasuhiro; Ikari, Akira; Kitamura, Naomi; Negishi, Masahiko; Miwa, Masao

    2008-01-01

    Constitutive androstane receptor (CAR) is a transcription factor to regulate the expression of several genes related to drug-metabolism. Here, we demonstrate that CAR protein accumulates during G1 in human SW480 and HepG2 cells. After the G1/S phase transition, CAR protein levels decreased, and CAR was hardly detected in cells by the late M phase. CAR expression in both cell lines was suppressed by RNA interference-mediated suppression of CDK4. Depletion of CAR by RNA interference in both cells and by hepatocyte growth factor treatment in HepG2 cells resulted in decreased MDM2 expression that led to p21 upregulation and repression of HepG2 cell growth. Thus, our results demonstrate that CAR expression is an early G1 event regulated by CDK4 that contributes to MDM2 expression; these findings suggest that CAR may influence the expression of genes involved in not only the metabolism of endogenous and exogenous substances but also in the cell proliferation

  17. Taraxacum officinale induces cytotoxicity through TNF-alpha and IL-1alpha secretion in Hep G2 cells.

    Science.gov (United States)

    Koo, Hyun-Na; Hong, Seung-Heon; Song, Bong-Keun; Kim, Cheorl-Ho; Yoo, Young-Hyun; Kim, Hyung-Min

    2004-01-16

    Taraxacum officinale (TO) has been frequently used as a remedy for women's disease (e.g. breast and uterus cancer) and disorders of the liver and gallbladder. Several earlier studies have indicated that TO exhibits anti-tumor properties, but its mechanism remains to be elucidated. In this study, we investigated the effect of TO on the cytotoxicity and production of cytokines in human hepatoma cell line, Hep G2. Our results show that TO decreased the cell viability by 26%, and significantly increased the tumor necrosis factor (TNF)-alpha and interleukin (IL)-1alpha production compared with media control (about 1.6-fold for TNF-alpha, and 2.4-fold for IL-1alpha, P < 0.05). Also, TO strongly induced apoptosis of Hep G2 cells as determined by flow cytometry. Increased amounts of TNF-alpha and IL-1alpha contributed to TO-induced apoptosis. Anti-TNF-alpha and IL-1alpha antibodies almost abolished it. These results suggest that TO induces cytotoxicity through TNF-alpha and IL-1alpha secretion in Hep G2 cells.

  18. A Novel Temperature Monitoring Sensor for Gas-Based Detectors in Large HEP Experiments

    Science.gov (United States)

    Benussi, L.; Bianco, S.; Caponero, M. A.; Colafranceschi, S.; Ferrini, M.; Felli, F.; Passamonti, L.; Pierluigi, D.; Polimadei, A.; Russo, A.; Saviano, G.; Vendittozzi, C.

    Gaseous detectors are commonly used in HEP (High Energy Physics) experiments to reconstruct the track of elementary particles. They are often made by a very large number of chambers with relatively small individual volume, arranged in thick layers placed approximately all around the vertex of the experiment in order to detect elementary particles produced in any direction. The large volume of gas inside the detector must be monitored for many parameters as they can affect both the efficiency and the working life of the detector. The temperature of the gas inside the individual chambers is a critical parameter to be monitored, as it can both affect the efficiency of the detector and point out on-board electronic circuitry overheating. In this paper we propose a novel gas temperature sensing system based on optical fibre technology. The adopted technology is well suited to make distributed sensing systems with large number of sensors, it is immune to electromagnetic disturbances and it has adequate radiation hardness. A prototype of the basic sensor of the proposed system was tested at the experimental facility for Resistive Plate Chamber characterization available at the INFN laboratories in Frascati. Results are presented and discussed.

  19. Necrosis of HepG2 cancer cells induced by the vibration of magnetic particles

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Biran [Laboratoire de Physique de la Matière Condensée (LPMC), CNRS UMR 7336, Université de Nice Sophia Antipolis, Parc Valrose, 06108 Nice (France); Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis, CNRS, 28 Avenue de Valrose, F-06100 Nice (France); Bienvenu, Céline [Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis, CNRS, 28 Avenue de Valrose, F-06100 Nice (France); Mendez-Garza, Juan; Lançon, Pascal; Madeira, Alexandra [Laboratoire de Physique de la Matière Condensée (LPMC), CNRS UMR 7336, Université de Nice Sophia Antipolis, Parc Valrose, 06108 Nice (France); Vierling, Pierre [Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis, CNRS, 28 Avenue de Valrose, F-06100 Nice (France); Di Giorgio, Christophe, E-mail: christophe.di-giorgio@unice.fr [Institut de Chimie de Nice, UMR 7272, Université de Nice Sophia Antipolis, CNRS, 28 Avenue de Valrose, F-06100 Nice (France); Bossis, Georges, E-mail: bossis@unice.fr [Laboratoire de Physique de la Matière Condensée (LPMC), CNRS UMR 7336, Université de Nice Sophia Antipolis, Parc Valrose, 06108 Nice (France)

    2013-10-15

    Experiments of magnetolysis, i.e., destruction of cells induced with magnetic particles (MPs) submitted to the application of a magnetic field, were conducted on HepG2 cancer cells. We herein demonstrate the usefulness of combining anisotropic MPs with an alternative magnetic field in magnetolysis. Thus, the application of an alternative magnetic field of low frequency (a few Hertz) in the presence of anisotropic, submicronic particles allowed the destruction of cancer cells “in vitro”. We also show that a constant magnetic field is far less efficient than an oscillating one. Moreover, we demonstrate that, at equal particle volume, it is much more efficient to utilize spindle shaped particles rather than spherical ones. In order to get deeper insight into the mechanism of magnetolysis experiments, we performed a study by AFM, which strongly supports that the magnetic field induces the formation of clusters of particles becoming then large enough todamage cell membranes. - Highlights: • Magnetic force was applied on cancer cells through magnetic particles. • The penetration depth was predicted, both for spherical and ellipsoidal particles. • Alternative force was shown to damage the cells contrary to static force. • The effect of indentation of magnetic particles was compared to the one of AFM tips. • The damage was attributed to the formation of clusters of particles.

  20. The Inspiring Science Education project and the resources for HEP analysis by university students

    International Nuclear Information System (INIS)

    Fassouliotis, Dimitris; Kourkoumelis, Christine; Vourakis, Stylianos

    2016-01-01

    The Inspiring Science Education outreach project has been running for more than two years, creating a large number of inquiry based educational resources for high-school teachers and students. Its goal is the promotion of science education in schools though new methods built on the inquiry based education techniques, involving large consortia of European partners and implementation of large-scale pilots in schools. Recent hands-on activities, developing and testing the above mentioned innovative applications are reviewed. In general, there is a lack for educational scenaria and laboratory courses earmarked for more advanced, namely university, students. At the University of Athens for the last four years, the HYPATIA on-line event analysis tool has been used as a lab course for fourth year undergraduate physics students, majoring in HEP. Up to now, the course was limited to visual inspection of a few tens of ATLAS events. Recently the course was enriched with additional analysis exercises, which involve large samples of events. The students through a user friendly interface can analyse the samples and optimize the cut selection in order to search for new physics. The implementation of this analysis is described

  1. Ignominy: a tool for software dependency and metric analysis with examples from large HEP packages

    International Nuclear Information System (INIS)

    Tuura, L.A.; Taylor, L.

    2001-01-01

    Ignominy is a tool developed in the CMS IGUANA project to analyse the structure of software systems. Its primary component is a dependency scanner that distills information into human-usable forms. It also includes several tools to visualise the collected data in the form of graphical views and numerical metrics. Ignominy was designed to adapt to almost any reasonable structure, and it has been used to analyse several large projects. The original purpose of Ignominy was to help us better ensure the quality of our own software, and in particular warn us about possible structural problems early on. As a part of this activity it is now used as a standard part of our release procedure. The authors also use it to evaluate and study the quality of external packages they plan to make use of. The authors describe what Ignominy can find out, and how it can be used to visualise and assess a software structure. The authors also discuss the inherent problems of the analysis as well as the different approaches to modularity the tool makes quite evident. The focus is the illustration of these issues through the analysis results for several sizable HEP software projects

  2. Surface ligand dependent toxicity of zinc oxide nanoparticles in HepG2 cell model

    International Nuclear Information System (INIS)

    Bartczak, D; Baradez, M-O; Merson, S; Goenaga-Infante, H; Marshall, D

    2013-01-01

    Physicochemical properties of nanoparticles (NP) strongly affect their influence on cell behaviour, but can be significantly distorted by interactions with the proteins present in biological solutions. In this study we show how different surface functionalities of zinc oxide (ZnO) NP lead to changes in the size distribution and dissolution of the NP in serum containing cell culture media and how this impacts on NP toxicity. NPs capped with weakly bound large proteins undergo substantial transformations due to the exchange of the original surface ligands to the components of the cell culture media. Conversely, NP capped with a tight monolayer of small organic molecules or with covalently conjugated proteins show significantly higher stability. These differences in ligand exchange also affect the toxicity of the NP to the HepG2 liver cell model, with the NP capped with small organic molecules being more toxic than those capped with large proteins. This study highlights the importance of characterising NPs in biological media and the effect the media has during in-vitro analysis.

  3. Antiproliferative effects of an analog of curcumin in Hep-2 cells: a comparative study with curcumin.

    Science.gov (United States)

    Kumaravel, Mohankumar; Sankar, Pajaniradje; Latha, Periyasamy; Benson, Chellakan S; Rukkumani, Rajagopalan

    2013-02-01

    Curcumin, the major active principle of Curcuma longa, is one of the promising, plant-derived, chemopreventive agents being studied for its anticarcinogenic and antioxidant properties. Hence, in our study, we aimed at testing the antiproliferative efficacy of an o-hydroxyl substituted analog of curcumin, bis demethoxy curcumin analog (BDMC-A), and comparing its efficacy with that of curcumin. BDMC-A was synthesised with a yield of 78% and 98% purity. Hep-2 cells and the MTT cell viability assay were used to examine cell proliferation. LDH assay and cell counts were performed to assess the cytotoxicity and anti-proliferative effects of the compound, respectively. Flow cytometry followed by Western blot were performed to investigate the cell cycle distribution. BDMC-A inhibited cell proliferation at a much lower concentration (IC50 20 microM) than curcumin (IC50 50 microM). Similar effects were observed in the LDH release and cell count assays. Flow cytometric studies using propidium iodide showed accumulation of cells in the G0/G1 phase and the arrest was further confirmed by immunoblotting of protein cyclin D1. BDMC-A was more potent in inhibiting the cells at a lower dose when compared with curcumin. Our results showed that the analog of curcumin is likely to possess more efficacy compared with curcumin in inhibiting cancer.

  4. Cholesterol-lowing effect of taurine in HepG2 cell.

    Science.gov (United States)

    Guo, Junxia; Gao, Ya; Cao, Xuelian; Zhang, Jing; Chen, Wen

    2017-03-16

    A number of studies indicate that taurine promotes cholesterol conversion to bile acids by upregulating CYP7A1 gene expression. Few in vitro studies are concerned the concentration change of cholesterol and its product of bile acids, and the molecular mechanism of CYP7A1 induction by taurine. The levels of intracellular total cholesterol (TC), free cholesterol (FC), cholesterol ester (EC), total bile acids (TBA) and medium TBA were determined after HepG2 cells were cultured for 24/48 h in DMEM supplemented with taurine at the final concentrations of 1/10/20 mM respectively. The protein expressions of CYP7A1, MEK1/2, c-Jun, p-c-Jun and HNF-4α were detected. Taurine significantly reduced cellular TC and FC in dose -and time-dependent ways, and obviously increased intracellular/medium TBA and CYP7A1 expressions. There was no change in c-Jun expression, but the protein expressions of MEK1/2 and p-c-Jun were increased at 24 h and inhibited at 48 h by 20 mM taurine while HNF4α was induced after both of the 24 h and 48 h treatment. Taurine could enhance CYP7A1 expression by inducing HNF4α and inhibiting MEK1/2 and p-c-Jun expressions to promote intracellular cholesterol metabolism.

  5. Proapoptotic activity of aflatoxin B1 and sterigmatocystin in HepG2 cells

    Directory of Open Access Journals (Sweden)

    Yang Liu

    2014-01-01

    Full Text Available Aflatoxin B1 (AFB1 and sterigmatocystin (ST are two hepatocarcinogenic mycotoxins that are commonly coexisted in cereal grains, and their co-proapoptotic activity in HepG2 cells was studied. The values of IC50, which is the dosage of mycotoxin resulting in a 50% cell growth inhibition measured by a sulforhodamine B (SRB colorimetric assay, were 16.9 μM and 7.3 μM for AFB1 and ST, respectively. Additively and dose-dependently, cell apoptosis-related toxicity endpoints of double strand DNA and ATP content were decreased while the intracellular ROS and mitochondria membrane permeability (MMP were increased. Consistently, when cell cycle is arrest at G0/G1 or S phase by AFB1 and/or ST, the experimental results from flow cytometry assay demonstrated that the rate of cell apoptosis and mitochondrial membrane potential were also additively increased and decreased, respectively, in a dose-dependent manner. Thus, the integrity of mitochondria (MMP and membrane potential that is the central component of cell apoptosis is disrupted by AFB1 and ST in an additive manner. With the immunocytochemistry analysis showing increased expression of apoptosis-related proteins of Bax, Caspase-3 and p53 and decreased expression of Bcl-2 protein, an additive nature of the co-proapoptotic activity of AFB1 and ST was revealed.

  6. Evaluation of anti-hepatocarcinoma capacity of puerarin nanosuspensions against human HepG2 cells

    Science.gov (United States)

    Meng, Xiang-Ping; Zhang, Zhen; Wang, Yi-Fei; Wang, Zhi-ping; Chen, Tong-sheng

    2017-02-01

    Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Puerarin (Pue), a major active ingredient in the traditional Chinese medicine Gegen, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Pue nanosuspension (Pue-NS) composed of Pue and poloxamer 188 was prepared by high pressure homogenization technique. The in vitro anti-hepatocarcinoma effects of Pue-NS relative to efficacy of bulk Pue were evaluated. The particle size and zeta potential of Pue-NS were 218.5 nm and -18.8 mV, respectively. MTT assay showed that Pue-NS effectively inhibited the proliferation of HepG2 cells, and the corresponding IC50 values of Pue-NS and bulk Pue were 3.39 and 5.73 μg/ml. These results suggest that the delivery of Pue-NS is a promising approach for treating tumors.

  7. A potent approach for the development of FPGA based DAQ system for HEP experiments

    International Nuclear Information System (INIS)

    Khan, Shuaib Ahmad; Mitra, Jubin; Nayak, Tapan Kumar; David, Erno; Kiss, Tivadar

    2017-01-01

    With ever increasing particle beam energies and interaction rates in modern High Energy Physics (HEP) experiments in the present and future accelerator facilities, there has always been the demand for robust Data Acquisition (DAQ) schemes which perform in the harsh radiation environment and handle high data volume. The scheme is required to be flexible enough to adapt to the demands of future detector and electronics upgrades, and at the same time keeping the cost factor in mind. To address these challenges, in the present work, we discuss an efficient DAQ scheme for error resilient, high speed data communication on commercially available state-of-the-art FPGA with optical links. The scheme utilises GigaBit Transceiver (GBT) protocol to establish radiation tolerant communication link between on-detector front-end electronics situated in harsh radiation environment to the back-end Data Processing Unit (DPU) placed in a low radiation zone. The acquired data are reconstructed in DPU which reduces the data volume significantly, and then transmitted to the computing farms through high speed optical links using 10 Gigabit Ethernet (10GbE). In this study, we focus on implementation and testing of GBT protocol and 10GbE links on an Intel FPGA. Results of the measurements of resource utilisation, critical path delays, signal integrity, eye diagram and Bit Error Rate (BER) are presented, which are the indicators for efficient system performance.

  8. Surface ligand dependent toxicity of zinc oxide nanoparticles in HepG2 cell model

    Science.gov (United States)

    Bartczak, D.; Baradez, M.-O.; Merson, S.; Goenaga-Infante, H.; Marshall, D.

    2013-04-01

    Physicochemical properties of nanoparticles (NP) strongly affect their influence on cell behaviour, but can be significantly distorted by interactions with the proteins present in biological solutions. In this study we show how different surface functionalities of zinc oxide (ZnO) NP lead to changes in the size distribution and dissolution of the NP in serum containing cell culture media and how this impacts on NP toxicity. NPs capped with weakly bound large proteins undergo substantial transformations due to the exchange of the original surface ligands to the components of the cell culture media. Conversely, NP capped with a tight monolayer of small organic molecules or with covalently conjugated proteins show significantly higher stability. These differences in ligand exchange also affect the toxicity of the NP to the HepG2 liver cell model, with the NP capped with small organic molecules being more toxic than those capped with large proteins. This study highlights the importance of characterising NPs in biological media and the effect the media has during in-vitro analysis.

  9. Importancia de las prostaglandinas en la amibiasis hepática

    Directory of Open Access Journals (Sweden)

    Sánchez-Ramírez Blanca

    2002-01-01

    Full Text Available Las prostaglandinas son importantes mediadores inflamatorios, pero también desempeñan un papel importante como reguladoras de las funciones de los linfocitos y los macrófagos. La inoculación por vía intrahepática o intraportal de trofozoitos viables de Entamoeba histolytica en hámsteres se caracteriza por una rápida respuesta inflamatoria aguda, en la cual los trofozoitos amibianos se ven rodeados sucesivamente por leucocitos polimorfonucleares, linfocitos y macrófagos. La incapacidad de estas células para contrarrestar la invasión amibiana ha sido demostrada en varios estudios. La prostaglandina E2 (PGE2 tiene potentes efectos sobre las células de la respuesta inmune; su participación durante la formación del absceso hepático se reportó recientemente. En este artículo hacemos una revisión de los hallazgos de los últimos años en relación con el estudio de los mediadores bioquímicos de la inflamación durante la infección con E. histolytica, y su posible participación en el establecimiento de la respuesta inmune en el huésped.

  10. Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesis

    Directory of Open Access Journals (Sweden)

    Dan Xu

    2016-07-01

    Full Text Available Deficiency in biosynthesis of inner core of lipopolysaccharide (LPS rendered a characteristic biofilm-forming phenotype in E. coli. The pathological implications of this new phenotype in Shigella flexneri, a highly contagious enteric Gram-negative bacteria that is closely related to E. coli, were investigated in this study. The ΔrfaC (also referred as waaC mutant, with incomplete inner core of LPS due to deficiency in Hep biosynthesis, was characteristic of strong biofilm formation ability and exhibited much more pronounced adhesiveness and invasiveness to human epithelial cells than the parental strain and other LPS mutants, which also showed distinct pattern of F-actin recruitment. Failure to cause keratoconjunctivitis and colonize in the intestine in guinea pigs revealed that the fitness gain on host adhesion resulted from biofilm formation is not sufficient to offset the loss of fitness on survivability caused by LPS deletion. Our study suggests a clear positive relationship between increased surface hydrophobicity and adhesiveness of Shigella flexneri, which should be put into consideration of virulence of Shigella, especially when therapeutic strategy targeting the core oligosaccharide (OS is considered an alternative to deal with bacterial antibiotics-resistance.

  11. Toxicity Study of Silver Nanoparticles Synthesized from Suaeda monoica on Hep-2 Cell Line.

    Science.gov (United States)

    Satyavani, Kaliyamurthi; Gurudeeban, Selvaraj; Ramanathan, Thiruganasambandam; Balasubramanian, Thangavel

    2012-01-01

    Recently there has been fabulous excitement in the nano-biotechnological area for the study of nanoparticles synthesis using some natural biological system, which has led the growth advanced nanomaterials. This intention made us to assess the biologically synthesized silver nanoparticles from the leaf of Suaeda monoica (S.monoica) using 1 mM silver nitrate. The leaf extract of S.monoica incubated with 1 mM silver nitrate solution and characterized by UV- spectrometer and AFM. The effect of synthesized silver nanoparticles on Human Epidermoid Larynx Carcinoma cell line was evaluated by the MTT colorimetric technique. As a result we observed gradual change in the colour of extract from greenish to brown. The synthesized silver nanoparticles confirmed by UV at 430 nm and spherical shape identified in the range of 31 nm under AFM. The effect of silver nanoparticles on Human Epidermoid Larynx Carcinoma cell line exhibits a dose-dependent toxicity for the cell tested and the viability of Hep-2 cells decreased to 50 % (IC(50)) at the concentration of 500 nM. Further findings will be determined the exact mechanisms of this cost effective Nano-treatments.

  12. An analysis of reproducibility and non-determinism in HEP software and ROOT data

    Science.gov (United States)

    Ivie, Peter; Zheng, Charles; Lannon, Kevin; Thain, Douglas

    2017-10-01

    Reproducibility is an essential component of the scientific method. In order to validate the correctness or facilitate the extension of a computational result, it should be possible to re-run a published result and verify that the same results are produced. However, reproducing a computational result is surprisingly difficult: non-determinism and other factors may make it impossible to get the same result, even when running the same code on the same machine on the same day. We explore this problem in the context of HEP codes and data, showing three high level methods for dealing with non-determinism in general: 1) Domain specific methods; 2) Domain specific comparisons; and 3) Virtualization adjustments. Using a CMS workflow with output data stored in ROOT files, we use these methods to prevent, detect, and eliminate some sources of non-determinism. We observe improved determinism using pre-determined random seeds, a predictable progression of system timestamps, and fixed process identifiers. Unfortunately, sources of non-determinism continue to exist despite the combination of all three methods. Hierarchical data comparisons also allow us to appropriately ignore some non-determinism when it is unavoidable. We conclude that there is still room for improvement, and identify directions that can be taken in each method to make an experiment more reproducible.

  13. Terpenoids from Curcuma wenyujin increased glucose consumption on HepG2 cells.

    Science.gov (United States)

    Zhou, Chang-Xin; Zhang, Li-Sha; Chen, Fei-Fei; Wu, Hao-Shu; Mo, Jian-Xia; Gan, Li-She

    2017-09-01

    Thirty four terpenoids, including two new cadinane-type sesquiterpenoids containing conjugated aromatic-ketone moieties, curcujinone A (1) and curcujinone B (2), were isolated from 95% ethanol extract of the root tubers of Curcuma wenyujin. Their structures were determined by spectroscopic methods, especially 2D NMR and HRMS techniques. The relative and absolute configurations of 1 and 2 were identified by quantum chemical DFT and TDDFT calculations of the 13 C NMR chemical shifts, ECD spectra, and specific optical rotations. All compounds and extracts were evaluated for their anti-diabetic activities with a glucose consumption model on HepG2 Cells. The petroleum fraction CWP (10μg/mL) and compounds curcumenol (4), 7α,11α-epoxy-5β-hydroxy-9-guaiaen-8-one (5), curdione (17), (1S, 4S, 5S 10S)-germacrone (18), zederone (20), a mixture of curcumanolide A (25) and curcumanolide B (26), gajutsulactone B (27), and wenyujinin C (30) showed promising activities with over 45% increasing of glucose consumption at 10μM. Copyright © 2017. Published by Elsevier B.V.

  14. A potent approach for the development of FPGA based DAQ system for HEP experiments

    Science.gov (United States)

    Khan, Shuaib Ahmad; Mitra, Jubin; David, Erno; Kiss, Tivadar; Nayak, Tapan Kumar

    2017-10-01

    With ever increasing particle beam energies and interaction rates in modern High Energy Physics (HEP) experiments in the present and future accelerator facilities, there has always been the demand for robust Data Acquisition (DAQ) schemes which perform in the harsh radiation environment and handle high data volume. The scheme is required to be flexible enough to adapt to the demands of future detector and electronics upgrades, and at the same time keeping the cost factor in mind. To address these challenges, in the present work, we discuss an efficient DAQ scheme for error resilient, high speed data communication on commercially available state-of-the-art FPGA with optical links. The scheme utilises GigaBit Transceiver (GBT) protocol to establish radiation tolerant communication link between on-detector front-end electronics situated in harsh radiation environment to the back-end Data Processing Unit (DPU) placed in a low radiation zone. The acquired data are reconstructed in DPU which reduces the data volume significantly, and then transmitted to the computing farms through high speed optical links using 10 Gigabit Ethernet (10GbE). In this study, we focus on implementation and testing of GBT protocol and 10GbE links on an Intel FPGA. Results of the measurements of resource utilisation, critical path delays, signal integrity, eye diagram and Bit Error Rate (BER) are presented, which are the indicators for efficient system performance.

  15. BES-HEP Connections: Common Problems in Condensed Matter and High Energy Physics, Round Table Discussion

    Energy Technology Data Exchange (ETDEWEB)

    Fradkin, Eduardo [Univ. of Illinois, Urbana, IL (United States); Maldacena, Juan [Inst. for Advanced Study, Princeton, NJ (United States); Chatterjee, Lali [Dept. of Energy (DOE), Washington DC (United States). Office of Science. Office of High Energy Physics; Davenport, James W [Dept. of Energy (DOE), Washington DC (United States). Office of Science. Office of Basic Energy Sciences

    2015-02-02

    On February 2, 2015 the Offices of High Energy Physics (HEP) and Basic Energy Sciences (BES) convened a Round Table discussion among a group of physicists on ‘Common Problems in Condensed Matter and High Energy Physics’. This was motivated by the realization that both fields deal with quantum many body problems, share many of the same challenges, use quantum field theoretical approaches and have productively interacted in the past. The meeting brought together physicists with intersecting interests to explore recent developments and identify possible areas of collaboration.... Several topics were identified as offering great opportunity for discovery and advancement in both condensed matter physics and particle physics research. These included topological phases of matter, the use of entanglement as a tool to study nontrivial quantum systems in condensed matter and gravity, the gauge-gravity duality, non-Fermi liquids, the interplay of transport and anomalies, and strongly interacting disordered systems. Many of the condensed matter problems are realizable in laboratory experiments, where new methods beyond the usual quasi-particle approximation are needed to explain the observed exotic and anomalous results. Tools and techniques such as lattice gauge theories, numerical simulations of many-body systems, and tensor networks are seen as valuable to both communities and will likely benefit from collaborative development.

  16. On the relevancy of efficient, integrated computer and network monitoring in HEP distributed online environment

    International Nuclear Information System (INIS)

    Carvalho, D.; Gavillet, Ph.; Delgado, V.; Javello, J.; Miere, Y.; Ruffinoni, D.; Albert, J.N.; Bellas, N.; Smith, G.

    1996-01-01

    Large Scientific Equipment are controlled by Computer Systems whose complexity is growing driven, on the one hand by the volume and variety of the information, its distributed nature, the sophistication of its treatment and, on the other hand by the fast evolution of the computer and network market. Some people call them generically Large-Scale Distributed Data Intensive Information Systems or Distributed Computer Control Systems (DCCS) for those systems dealing more with real time control. Taking advantage of (or forced by) the distributed architecture, the tasks are more and more often implemented as Client-Server applications. In this framework the monitoring of the computer nodes, the communications network and the applications becomes of primary importance for ensuring the the safe running and guaranteed performance of the system. With the future generation of HEP experiments, such as those at the LHC in view, it is proposed to integrate the various functions of DCCS monitoring into one general purpose Multi-layer System. (author)

  17. Application of State Quantization-Based Methods in HEP Particle Transport Simulation

    Science.gov (United States)

    Santi, Lucio; Ponieman, Nicolás; Jun, Soon Yung; Genser, Krzysztof; Elvira, Daniel; Castro, Rodrigo

    2017-10-01

    Simulation of particle-matter interactions in complex geometries is one of the main tasks in high energy physics (HEP) research. An essential aspect of it is an accurate and efficient particle transportation in a non-uniform magnetic field, which includes the handling of volume crossings within a predefined 3D geometry. Quantized State Systems (QSS) is a family of numerical methods that provides attractive features for particle transportation processes, such as dense output (sequences of polynomial segments changing only according to accuracy-driven discrete events) and lightweight detection and handling of volume crossings (based on simple root-finding of polynomial functions). In this work we present a proof-of-concept performance comparison between a QSS-based standalone numerical solver and an application based on the Geant4 simulation toolkit, with its default Runge-Kutta based adaptive step method. In a case study with a charged particle circulating in a vacuum (with interactions with matter turned off), in a uniform magnetic field, and crossing up to 200 volume boundaries twice per turn, simulation results showed speedups of up to 6 times in favor of QSS while it being 10 times slower in the case with zero volume boundaries.

  18. Radiation hardness of semiconductor avalanche detectors for calorimeters in future HEP experiments

    Science.gov (United States)

    Kushpil, V.; Mikhaylov, V.; Kugler, A.; Kushpil, S.; Ladygin, V. P.; Svoboda, O.; Tlustý, P.

    2016-02-01

    During the last years, semiconductor avalanche detectors are being widely used as the replacement of classical PMTs in calorimeters for many HEP experiments. In this report, basic selection criteria for replacement of PMTs by solid state devices and specific problems in the investigation of detectors radiation hardness are discussed. The design and performance of the hadron calorimeters developed for the future high energy nuclear physics experiments at FAIR, NICA, and CERN are discussed. The Projectile Spectator Detector (PSD) for the CBM experiment at the future FAIR facility, the Forward Calorimeter for the NA61 experiment at CERN and the Multi Purpose Detector at the future NICA facility are reviewed. Moreover, new methods of data analysis and results interpretation for radiation experiments are described. Specific problems of development of detectors control systems and possibilities of reliability improvement of multi-channel detectors systems are shortly overviewed. All experimental material is based on the investigation of SiPM and MPPC at the neutron source in NPI Rez.

  19. A 166.6 MHz superconducting rf system for the HEPS storage ring

    Science.gov (United States)

    Zhang, P.; Hao, X.; Huang, T.; Li, Z.; Lin, H.; Meng, F.; Mi, Z.; Sun, Y.; Wang, G.; Wang, Q.; Zhang, X.

    2017-07-01

    A superconducting 166.6 MHz quarter-wave beta=1 cavity was recently proposed for the High Energy Photon Source (HEPS), a 6 GeV kilometer-scale light source. Four 166.6 MHz cavities will be used for main acceleration in the newly planned on-axis beam injection scheme realized by a double-frequency RF system. The fundamental frequency, 166.6 MHz, was dictated by the fast injection kicker technology and the preference of using 499.8 MHz SC RF cavity as the third harmonic. Each 166.6 MHz cavity will be operated at 4.2 K providing 1.2 MV accelerating voltage and 150 kW of power to the electron beam. The input coupler will use single-window coaxial type graded up to 200 kW CW power. Each cavity will be equipped with a 200 kW solid-state amplifier and digital low-level RF system. This paper describes the 166.6 MHz RF system with a focus on the design and optimization of the RF cavity and its ancillaries, the LLRF system and the status of the solid-state amplifiers.

  20. Performance awareness execution performance of HEP codes on RISC platforms,issues and solutions

    CERN Document Server

    Yaari, R; Yaari, Refael; Jarp, Sverre

    1995-01-01

    The work described in this paper was started during the migration of Aleph's production jobs from the IBM mainframe/CRAY supercomputer to several RISC/Unix workstation platforms. The aim was to understand why Aleph did not obtain the performance on the RISC platforms that was "promised" after a CERN Unit comparison between these RISC platforms and the IBM mainframe. Remedies were also sought. Since the work with the Aleph jobs in turn led to the related task of understanding compilers and their options, the conditions under which the CERN benchmarks (and other benchmarks) were run, kernel routines and frequently used CERNLIB routines, the whole undertaking expanded to try to look at all the factors that influence the performance of High Energy Physics (HEP) jobs in general. Finally, key performance issues were reviewed against the programs of one of the LHC collaborations (Atlas) with the hope that the conclusions would be of long- term interest during the establishment of their simulation, reconstruction and...

  1. Cimicifuga foetida L. inhibited human respiratory syncytial virus in HEp-2 and A549 cell lines.

    Science.gov (United States)

    Wang, Kuo Chih; Chang, Jung San; Chiang, Lien Chai; Lin, Chun Ching

    2012-01-01

    Human respiratory syncytial virus (HRSV) causes serious pediatric infection of the lower respiratory tract without effective therapeutic modality. Sheng-Ma-Ge-Gen-Tang (SMGGT; Shoma-kakkon-to) has been proven to be effective at inhibiting HRSV-induced plaque formation, and Cimicifuga foetida is the major constituent of SMGGT. We tested the hypothesis that C. foetida effectively inhibited the cytopathic effects of HRSV by a plaque reduction assay in both human upper (HEp2) and lower (A549) respiratory tract cell lines. Its ability to stimulate anti-viral cytokines was evaluated by an enzyme-linked immunosorbent assay (ELISA). C. foetida dose-dependently inhibited HRSV-induced plaque formation (p < 0.0001) before and after viral inoculation, especially in A549 cells (p < 0.0001). C. foetida dose-dependently inhibited viral attachment (p < 0.0001) and could increase heparins effect on viral attachment. In addition, C. foetida time-dependently and dose-dependently (p < 0.0001) inhibited HRSV internalization. C. foetida could stimulate epithelial cells to secrete IFN-β to counteract viral infection. However, C. foetida did not stimulate TNF-α secretion. Therefore, C. foetida could be useful in managing HRSV infection. This is the first evidence to support that C. foetida possesses antiviral activity.

  2. In Vitro Characterization and Evaluation of the Cytotoxicity Effects of Nisin and Nisin-Loaded PLA-PEG-PLA Nanoparticles on Gastrointestinal (AGS and KYSE-30), Hepatic (HepG2) and Blood (K562) Cancer Cell Lines.

    Science.gov (United States)

    Goudarzi, Fariba; Asadi, Asadollah; Afsharpour, Maryam; Jamadi, Robab Hassanvand

    2018-02-22

    The aim of this study was an in vitro evaluation and comparison of the cytotoxic effects of free nisin and nisin-loaded PLA-PEG-PLA nanoparticles on gastrointestinal (AGS and KYSE-30), hepatic (HepG2), and blood (K562) cancer cell lines. To create this novel anti-cancer drug delivery system, the nanoparticles were synthesized and then loaded with nisin. Subsequently, their biocompatibility, ability to enter cells, and physicochemical properties, including formation, size, and shape, were studied using hemolysis, fluorescein isothiocyanate (FITC), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), and scanning electron microscopy (SEM), respectively. Then, its loading efficiency and release kinetics were examined to assess the potential impact of this formulation for the nanoparticle carrier candidacy. The cytotoxicities of nisin and nisin-loaded nanoparticles were evaluated by using the MTT and Neutral Red (NR) uptake assays. Detections of the apoptotic cells were done via Ethidium Bromide (EB)/Acridine Orange (AO) staining. The FTIR spectra, SEM images, and DLS graph confirmed the formations of the nanoparticles and nisin-loaded nanoparticles with spherical, distinct, and smooth surfaces and average sizes of 100 and 200 nm, respectively. The loading efficiency of the latter nanoparticles was about 85-90%. The hemolysis test represented their non-cytotoxicities and the FITC images indicated their entrance inside the cells. An increase in the percentage of apoptotic cells was observed through EB/AO staining. These results demonstrated that nisin had a cytotoxic effect on AGS, KYSE-30, HepG2, and K562 cancer cell lines, while the cytotoxicity of nisin-loaded nanoparticles was more than that of the free nisin.

  3. Stimulation of LDL receptor activity in Hep-G2 cells by a serum factor(s)

    International Nuclear Information System (INIS)

    Ellsworth, J.L.; Brown, C.; Cooper, A.D.

    1988-01-01

    The regulation of low-density lipoprotein (LDL) receptor activity in the human hepatoma cell line Hep-G2 by serum components was examined. Incubation of dense monolayers of Hep-G2 cells with fresh medium containing 10% fetal calf serum (FM) produced a time-dependent increase in LDL receptor activity. Uptake and degradation of 125I-LDL was stimulated two- to four-fold, as compared with that of Hep-G2 cells cultured in the same media in which they had been grown to confluence (CM); the maximal 125I-LDL uptake plus degradation increased from 0.2 microgram/mg cell protein/4 h to 0.8 microgram/mg cell protein/4 h. In addition, a two-fold increase in cell surface binding of 125I-LDL to Hep-G2 cells was observed when binding was measured at 4 degrees C. There was no change in the apparent Kd. The stimulation of LDL receptor activity was suppressed in a concentration-dependent manner by the addition of cholesterol, as LDL, to the cell medium. In contrast to the stimulation of LDL receptor activity, FM did not affect the uptake or degradation of 125I-asialoorosomucoid. Addition of FM increased the protein content per dish, and DNA synthesis was stimulated approximately five-fold, as measured by [3H]thymidine incorporation into DNA; however, the cell number did not change. Cellular cholesterol biosynthesis was also stimulated by FM; [14C]acetate incorporation into unesterified and esterified cholesterol was increased approximately five-fold. Incubation of Hep-G2 cells with high-density lipoproteins (200 micrograms protein/ml) or albumin (8.0 mg/ml) in the absence of the serum factor did not significantly increase the total processed 125I-LDL. Stimulation of LDL receptor activity was dependent on a heat-stable, nondialyzable serum component that eluted in the inclusion volume of a Sephadex G-75 column

  4. Alteração de enzimas hepáticas em trabalhadores de refinaria de petróleo

    Directory of Open Access Journals (Sweden)

    Carvalho Fernando Martins

    2006-01-01

    Full Text Available OBJETIVO: A exposição ocupacional típica de uma refinaria de petróleo pode alterar a função hepática de seus trabalhadores. Assim, o objetivo do estudo foi identificar fatores de risco de alterações em enzimas hepáticas em trabalhadores de uma refinaria de petróleo. MÉTODOS: Os trabalhadores de uma refinaria de petróleo, localizada em São Francisco do Conde, Estado da Bahia, eram submetidos a exames periódicos anuais de 1982 a1998. O estudo caso-controle investigou todos os 150 casos de indivíduos com alteração simultânea de gama-glutamil transferase e de alanino amino transferase, de pelo menos 10% acima do valor de referência. Como controles, foram selecionados 150 indivíduos sem quaisquer alterações de enzimas hepáticas ou de bilirrubinas, desde a sua admissão. Foram calculadas as razões de chance e respectivos intervalos de confiança de 95% a partir de modelos de regressão logística. RESULTADOS: Em todos os setores de produção, o risco de alteração de enzimas hepáticas foi significantemente mais elevado do que no setor administrativo (RC=5,7; IC 95%: 1,7-18,4, estando controlados os efeitos do álcool, obesidade e antecedentes médicos de hepatite. No período 1992-1994 foram registrados 89 casos, 88 deles provieram dos diversos setores da produção. CONCLUSÕES: A exposição ocupacional desempenha papel importante na determinação de alterações de enzimas hepáticas em trabalhadores do refino de petróleo, além dos fatores de risco eminentemente biológicos e/ou comportamentais como obesidade e o consumo de álcool.

  5. Modulation of the pentose phosphate pathway alters phase I metabolism of testosterone and dextromethorphan in HepG2 cells.

    Science.gov (United States)

    Xiao, Wen-jing; Ma, Ting; Ge, Chun; Xia, Wen-juan; Mao, Yong; Sun, Run-bin; Yu, Xiao-yi; Aa, Ji-ye; Wang, Guang-ji

    2015-02-01

    The pentose phosphate pathway (PPP) is involved in the activity of glucose-6-phosphate dehydrogenase (G6PD) and generation of NADPH, which plays a key role in drug metabolism. The aim of this study was to investigate the effects of modulation of the PPP on drug metabolism capacity in vitro. A pair of hepatic cell lines, ie, the cancerous HepG2 cells and normal L02 cells, was used. The expression of CYP450 enzymes, p53 and G6PD in the cells were analyzed. The metabolism of testosterone (TEST, 10 μmol/L) and dextromethorphan (DEM, 1 μmol/L), the two typical substrates for CYP3A4 and CYP2D6, in the cells was examined in the presence of different agents. Both the expression and metabolic activities of CYP3A4 and CYP2D6 were considerably higher in HepG2 cells than in L02 cells. The metabolism of TEST and DEM in HepG2 cells was dose-dependently inhibited by the specific CYP3A4 inhibitor ketoconazole and CYP2D6 inhibitor quinidine. Addition of the p53 inhibitor cyclic PFT-α (5, 25 μmol/L) in HepG2 cells dose-dependently enhanced the metabolism of DEM and TEST, whereas addition of the p53 activator NSC 66811 (3, 10, 25 μmol/L) dose-dependently inhibited the metabolism. Furthermore, addition of the G6PD inhibitor 6-aminonicotinamide (5, 15 μmol/L) in HepG2 cells dose-dependently inhibited the metabolism of DEM and TEST, whereas addition of the PPP activity stimulator menadione (1, 5, 15 μmol/L) dose-dependently enhanced the metabolism. Modulation of p53 and the PPP alters the metabolism of DEM and TEST, suggesting that the metabolic flux pattern of PPP may be closely involved in drug metabolism and the individual variance.

  6. Haemophilia & Exercise Project (HEP): the impact of 1-year sports therapy programme on physical performance in adult haemophilia patients.

    Science.gov (United States)

    Czepa, D; von Mackensen, S; Hilberg, T

    2013-03-01

    Episodes of bleeding in people with haemophilia (PWH) are associated with reduced activity and limitations in physical performance. Within the scope of the 'Haemophilia & Exercise Project' (HEP) PWH were trained in a sports therapy programme. Aim of this study was to investigate subjective and objective physical performance in HEP-participants after 1 year training. Physical performance of 48 adult PWH was compared before and after sports therapy subjectively (HEP-Test-Q) and objectively regarding mobility (range of motion), strength and coordination (one-leg-stand) and endurance (12-min walk test). Sports therapy included an independent home training that had previously been trained in several collective sports camps. Forty-three controls without haemophilia and without training were compared to PWH. Of 48 PWH, 13 performed a regular training (active PWH); 12 HEP-participants were constantly passive (passive PWH). Twenty-three PWH and 24 controls dropped out because of incomplete data. The activity level increased by 100% in active PWH and remained constant in passive PWH, and in controls (P ≤ 0.05). Only mobility of the right knee was significantly improved in active PWH (+5.8 ± 5.3°) compared to passive PWH (-1.3 ± 8.6°). The 12-min walk test proved a longer walking distance for active PWH (+217 ± 199 m) compared to controls (-32 ± 217 m). Active PWH reported a better subjective physical performance in the HEP-Test-Q domains 'strength & coordination', 'endurance' and in the total score (+9.4 ± 13.8) compared to passive PWH (-5.3 ± 13.5) and controls (+3.7 ± 7.5). The 'mobility'-scale and one-leg-stand remained unchanged. Sports therapy increases the activity level and physical performance of PWH, whereby objective effects do not always correspond with subjective assessments. © 2012 Blackwell Publishing Ltd.

  7. Hypertonicity-induced cation channels in HepG2 cells: architecture and role in proliferation vs. apoptosis.

    Science.gov (United States)

    Koos, Björn; Christmann, Jens; Plettenberg, Sandra; Käding, Domenic; Becker, Julia; Keteku, Melody; Klein, Christian; Imtiaz, Sarah; Janning, Petra; Bastiaens, Philippe I H; Wehner, Frank

    2018-01-25

    Na + conducting hypertonicity-induced cation channels (HICCs) are key players in the volume restoration of osmotically shrunken cells and, under isotonic conditions, considered as mediators of proliferation - thereby opposing apoptosis. In an siRNA screen of ion channels and transporters in HepG2 cells, with the regulatory volume increase (RVI) as read-out, δENaC, TRPM2 and TRPM5 were identified as HICCs. Subsequently, all permutations of these channels were tested in RVI and patch-clamp recordings and, at first sight, HICCs were found to operate in an independent mode. However, there was synergy in the siRNA perturbations of HICC currents. Accordingly, proximity ligation assays showed that δENaC was located in proximity to TRPM2 and TRPM5 suggesting a physical interaction. Furthermore, δENaC, TRPM2 and TRPM5 were identified as mediators of HepG2 proliferation - their silencing enhanced apoptosis. Our study defines the architecture of HICCs in human hepatocytes as well as their molecular functions. Hypertonicity-induced cation channels (HICCs) are a substantial element in the regulatory volume increase (RVI) of osmotically shrunken cells. Under isotonic conditions, they are key effectors in the volume gain preceding proliferation; HICC repression, in turn, significantly increases apoptosis rates. Despite these fundamental roles of HICCs in cell physiology, very little is known concerning the actual molecular architecture of these channels. Here, an siRNA screening of putative ion channels and transporters was performed, in HepG2 cells, with the velocity of RVI as the read-out; in this first run, δENaC, TRPM2 and TRPM5 could be identified as HICCs. In the second run, all permutations of these channels were tested in RVI and patch-clamp recordings, with special emphasis on the non-additivity and additivity of siRNAs - which would indicate molecular interactions or independent ways of channel functioning. At first sight, the HICCs in HepG2 cells appeared to

  8. C-fos upregulates P-glycoprotein, contributing to the development of multidrug resistance in HEp-2 laryngeal cancer cells with VCR-induced resistance.

    Science.gov (United States)

    Li, Guodong; Hu, Xiaoling; Sun, Lu; Li, Xin; Li, Jianfeng; Li, Tongli; Zhang, Xiaohui

    2018-01-01

    Laryngeal cancer tends to have a very poor prognosis due to the unsatisfactory efficacy of chemotherapy for this cancer. Multidrug resistance (MDR) is the main cause of chemotherapy failure. The proto-oncogene c-fos has been shown to be involved in the development of MDR in several tumor types, but few studies have evaluated the relationship between c-fos and MDR in laryngeal cancer. We investigated the role of c-fos in MDR development in laryngeal cancer cells (cell line: human epithelial type 2, HEp-2) using the chemotherapeutic vincristine (VCR). HEp-2/VCR drug resistance was established by selection against an increasing drug concentration gradient. The expressions of c-fos and multidrug resistance 1 (mdr1) were measured using qPCR and western blot. C-fos overexpression or knockdown was performed in various cells. The intracellular rhodamine-123 (Rh-123) accumulation assay was used to detect the transport capacity of P-glycoprotein (P-gp, which is encoded by the mdr1 gene). HEp-2 cells with VCR-induced resistance (HEp-2/VCR cells) were not only resistant to VCR but also evolved cross-resistance to other chemotherapeutic drugs. The expressions of the c-fos and mdr1genes were significantly higher in the HEp-2/VCR cells than in control cells. C-fos overexpression in HEp-2 cells (c-fos WT) resulted in increased P-gp expression and increased the IC 50 for 5-FU. C-fos knockdown in the HEp-2/VCR cells (c-fos shRNA) resulted in decreased P-gp expression and decreased IC 50 for 5-FU. An intracellular Rh-123 accumulation assay showed that the mean intracellular fluorescence intensity (MFI) was lower in the HEp-2/VCR cells than in HEp-2 cells. C-fos WT cells also showed lower MFI. By contrast, c-fos shRNA cells exhibited a higher MFI than the control group. C-fos increased the expression of P-gp and mdr1 in the HEp-2/VCR cells, and enhanced the efflux function of the cells, thereby contributing to the development of MDR.

  9. Effects of PARP-1 inhibitors AG-014699 and AZD2281 on proliferation and apoptosis of human hepatoma cell line HepG2

    Directory of Open Access Journals (Sweden)

    DU Senrong

    2015-06-01

    Full Text Available ObjectiveTo observe the inhibitory and pro-apoptotic effects of two poly(ADP-ribose polymerase (PARP-1 inhibitors, AG-014699 and AZD2281, on human hepatoma HepG2 cells and preliminarily explore the mechanism by which AG-014699 induces HepG2 cell apoptosis, and to provide a new therapeutic target for hepatoma. MethodsThe effects of different concentrations of AG-014699 and AZD2281 on HepG2 cell proliferation were determined by MTT assay. The cell apoptosis rate was measured by flow cytometry. The expression levels of caspase-3 and caspase-8 were measured by Western Blot. Inter-group comparison was made by t test. ResultsBoth AG-014699 and AZD2281 suppressed HepG2 cell proliferation in a time- and dose-dependent manner. However, the sensitivity of HepG2 cells to the two PARP-1 inhibitors was different. The half-maximal inhibitory concentrations of AG-014699 and AZD2281 at 48 h determined by MTT assay were about 20 μmol/L and 400 μmol/L, respectively. Flow cytometry and Western blot were not used to evaluate the apoptosis of HepG2 cells exposed to AZD2281 to which these cells were not sensitive. HepG2 cell apoptosis could be induced by 10, 30, and 50 μmol/L AG-014699, and the highest apoptosis rate at 48 h was significantly higher than that of the control group (3100%±2.13% vs 09%±0013%, P<0.01. Compared with those in the control group, the protein levels of caspase-3 and caspase-8 in HepG2 cells after 48-h exposure to 30, and 50 μmol/L AG-014699 increased. ConclusionThe two PARP-1 inhibitors AG-014699 and AZD2281 can inhibit the proliferation of HepG2 cells, which showed different sensitivities to the two inhibitors. AG-014699 can induce HepG2 cell apoptosis by up-regulating the protein expression of caspase-3 and caspase-8.

  10. Role of cytochrome P450 2J2 on cell proliferation and resistance to an anticancer agent in hepatocellular carcinoma HepG2 cells.

    Science.gov (United States)

    Hwang, Geun Hye; Park, So Mi; Han, Ho Jae; Baek, Kyoung Min; Kim, Joong Sun; Chang, Woochul; Lee, Ho Jin; Yun, Seung Pil; Ryu, Jung Min; Lee, Min Young

    2017-11-01

    The present study examined the role of human cytochrome P450 2J2 (CYP2J2) on cell proliferation and resistance to an anticancer agent using stable hepatocellular carcinoma HepG2 cells overexpressing CYP2J2. Overexpression of CYP2J2 significantly increased HepG2 cell proliferation and the expression levels of cell cycle regulatory proteins, including cyclin D1, cyclin E, cyclin-dependent kinase (Cdk)2 and Cdk4. CYP2J2-overexpressing HepG2 cells exhibited high levels of Akt phosphorylation compared with those observed in wild-type HepG2 cells. Although Akt phosphorylation in both cell lines was significantly attenuated by LY294002, a specific phosphoinositide 3-kinase/Akt signaling inhibitor, the levels of Akt phosphorylation following treatment with LY294002 were higher in CYP2J2-overexpressing HepG2 cells than in wild-type HepG2 cells. Cell counting revealed that proliferation was reduced by LY294002 in both cell lines; however, CYP2J2-overexpressing HepG2 cell numbers were higher than those of wild-type HepG2 cells following treatment with LY294002. These results indicated that increased cell proliferation by CYP2J2 overexpression is mediated by increased Akt activity. It was also demonstrated that doxorubicin, an anticancer agent, reduced cell viability, induced a significant increase in the B-cell lymphoma (Bcl)-2 associated X protein (Bax)/Bcl-2 ratio and decreased pro-caspase-3 levels in wild-type HepG2 cells. However, the doxorubicin-induced reduction in cell viability was significantly attenuated by enhanced upregulation of CYP2J2 expression. The increase in the Bax/Bcl-2 ratio and the decrease in pro-caspase-3 levels were also recovered by CYP2J2 overexpression. In conclusion, CYP2J2 serves important roles in cancer cell proliferation and resistance to the anticancer agent doxorubicin in HepG2 cells.

  11. The targeted inhibition of mitochondrial Hsp90 overcomes the apoptosis resistance conferred by Bcl-2 in Hep3B cells via necroptosis

    International Nuclear Information System (INIS)

    Yan, Chunlan; Oh, Joon Seok; Yoo, Seung Hee; Lee, Jee Suk; Yoon, Young Geol; Oh, Yoo Jin; Jang, Min Seok; Lee, Sang Yeob; Yang, Jun; Lee, Sang Hwa; Kim, Hye Young; Yoo, Young Hyun

    2013-01-01

    Previous studies have reported that a Gamitrinib variant containing triphenylphosphonium (G-TPP) binds to mitochondrial Hsp90 and rapidly inhibits its activity, thus inducing the apoptotic pathway in the cells. Accordingly, G-TPP shows a potential as a promising drug for the treatment of cancer. A cell can die from different types of cell death such as apoptosis, necrosis, necroptosis, and autophagic cell death. In this study, we further investigated the mechanisms and modes of cell death in the G-TPP-treated Hep3B and U937 cell lines. We discovered that G-TPP kills the U937 cells through the apoptotic pathway and the overexpression of Bcl-2 significantly inhibits U937 cell death to G-TPP. We further discovered that G-TPP kills the Hep3B cells by activating necroptosis in combination with the partial activation of caspase-dependent apoptosis. Importantly, G-TPP overcomes the apoptosis resistance conferred by Bcl-2 in Hep3B cells via necroptosis. We also observed that G-TPP induces compensatory autophagy in the Hep3B cell line. We further found that whereas there is a Bcl-2-Beclin 1 interaction in response to G-TPP, silencing the beclin 1 gene failed to block LC3-II accumulation in the Hep3B cells, indicating that G-TPP triggers Beclin 1-independent protective autophagy in Hep3B cells. Taken together, these data reveal that G-TPP induces cell death through a combination of death pathways, including necroptosis and apoptosis, and overcomes the apoptosis resistance conferred by Bcl-2 in Hep3B cells via necroptosis. These findings are important for the therapeutic exploitation of necroptosis as an alternative cell death program to bypass the resistance to apoptosis. Highlights: ► G-TPP binds to mitochondrial Hsp90. ► G-TPP induces apoptosis in U937 human leukemia cancer cells. ► G-TPP induces combination of death pathways in Hep3B cell. ► G-TPP overcomes the resistance conferred by Bcl-2 in Hep3B cells via necroptosis. ► G-TPP triggers Beclin 1-independent

  12. Veias do sistema porta-hepático em gansos domésticos Veins from hepatic portal vein system in domestic geese

    Directory of Open Access Journals (Sweden)

    Tatiana C. Santos

    2009-04-01

    Full Text Available A distribuição intraparenquimal das veias porta-hepáticas foi estudada em 30 gansos domésticos. Latex Neoprene corado foi injetado pela veia isquiática e os animais forma fixados por imersão e injeção intramuscular com formol a 10% e dissecados. O fígado esteve composto por um grande lobo hepático direito e por um lobo hepático esquerdo menor, os quais estiveram conectados por uma ponte de parênquima. O lobo direito do fígado teve exclusivamente vasos do sistema porta-hepático formados pela distribuição intraparenquimal da veia porta-hepática direita, enquanto que no lobo esquerdo estes originaram-se da veia porta-hepática direita e de pequenas veias porta-hepáticas esquerdas. A veia porta-hepática direita emitiu o ramo caudal direito, que emitiu um pequeno ramo caudolateral direito e um grande ramo caudomedial direito. Cranialmente esta veia emitiu os ramos craniais direito e ramos lateral direito. A porção transversa da veia porta-hepática direita cruzou para o lobo hepático esquerdo, emitindo de 1 a 6 pequenos ramos craniais e caudais para a região média do fígado. No lobo esquerdo, o ramo esquerdo da veia porta-hepática direita emitiu o ramo cranial esquerdo, o ramo lateral esquerdo e o ramo medial. De 1 a 6 veias porta-hepáticas esquerdas foram identificadas desembocando ou no ramo esquerdo da veia porta-hepática direita ou em sua porção transversa, oriundos do ventrículo gástrico e do pró-ventrículo. Em 40% dos gansos uma veia porta-hepática própria oriunda da confluência de vasos venosos da face esquerda do ventrículo distribuiu-se na extremidade caudal do lobo esquerdo isoladamente.The intraparenchymal distribution of the hepatic portal veins in 30 domestic geese were studied. Stained Neoprene latex was injected into the isquiatic vessels, and the animals were fixed in 10% formaldehyde by immersion and intramuscular injection. The liver of geese was composed of a large right and a smaller left

  13. Hepatoma SK Hep-1 cells exhibit characteristics of oncogenic mesenchymal stem cells with highly metastatic capacity.

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    Jong Ryeol Eun

    Full Text Available SK Hep-1 cells (SK cells derived from a patient with liver adenocarcinoma have been considered a human hepatoma cell line with mesenchymal origin characteristics, however, SK cells do not express liver genes and exhibit liver function, thus, we hypothesized whether mesenchymal cells might contribute to human liver primary cancers. Here, we characterized SK cells and its tumourigenicity.We found that classical mesenchymal stem cell (MSC markers were presented on SK cells, but endothelial marker CD31, hematopoietic markers CD34 and CD45 were negative. SK cells are capable of differentiate into adipocytes and osteoblasts as adipose-derived MSC (Ad-MSC and bone marrow-derived MSC (BM-MSC do. Importantly, a single SK cell exhibited a substantial tumourigenicity and metastatic capacity in immunodefficient mice. Metastasis not only occurred in circulating organs such as lung, liver, and kidneys, but also in muscle, outer abdomen, and skin. SK cells presented greater in vitro invasive capacity than those of Ad-MSC and BM-MSC. The xenograft cells from subcutaneous and metastatic tumors exhibited a similar tumourigenicity and metastatic capacity, and showed the same relatively homogenous population with MSC characteristics when compared to parental SK cells. SK cells could unlimitedly expand in vitro without losing MSC characteristics, its tumuorigenicity and metastatic capacity, indicating that SK cells are oncogenic MSC with enhanced self-renewal capacity. We believe that this is the first report that human MSC appear to be transformed into cancer stem cells (CSC, and that their derivatives also function as CSCs.Our findings demonstrate that SK cells represent a transformation mechanism of normal MSC into an enhanced self-renewal CSC with metastasis capacity, SK cells and their xenografts represent a same relative homogeneity of CSC with substantial metastatic capacity. Thus, it represents a novel mechanism of tumor initiation, development and

  14. Hepsoft - an approach for up to date multi-platform deployment of HEP specific software

    International Nuclear Information System (INIS)

    Roiser, S

    2011-01-01

    LHC experiments are depending on a rich palette of software components to build their specific applications. These underlying software components include the ROOT analysis framework, the Geant4 simulation toolkit, Monte Carlo generators, grid middle-ware, graphics libraries, scripting languages, databases, tools, etc. which are provided centrally in up to date versions on multiple platforms (Linux, Mac, Windows). Until recently this set of packages has been tested and released in a tree like structure as a consistent set of versions across operating systems, architectures and compilers for LHC experiments only. Because of the tree like deployment these releases were only usable in connection with a configuration management tool which provided the proper build and run-time environments and was hindering other parties outside LHC from easily using this palette of packages. In a new approach the releases will be grouped in 'flat structure' such that interested parties can start using it without configuration management, retaining all the above mentioned advantages. In addition to an increased usability the software shall also be distributed via system provided package deployment systems (rpm, apt, etc.). The approach of software deployment is following the ideas of providing a wide range of HEP specific software packages and tools in a coherent, up to date and modular way on multiple platforms. The target audience for such software deployments are individual developers or smaller development groups / experiments who don't have the resources to maintain this kind of infrastructure. This new software deployment strategy has already been successfully implemented for groups at CERN.

  15. Toxicity evaluation of surface water treated with different disinfectants in HepG2 cells.

    Science.gov (United States)

    Marabini, Laura; Frigerio, Silvia; Chiesara, Enzo; Radice, Sonia

    2006-01-01

    It is well known that water disinfection through chlorination causes the formation of a mixture of disinfection by-products (DBPs), many of which are genotoxic and carcinogenic. To demonstrate the formation of such compounds, a pilot water plant supplied with water from Lake Trasimeno was set up at the waterworks of Castiglione del Lago (PG, Italy). The disinfectants, continuously added to pre-filtered lake water flowing into three different basins, were sodium hypochlorite, chlorine dioxide and peracetic acid, an alternative disinfectant used until now for disinfecting waste waters, but not yet studied for a possible use in drinking water treatment. The aim of this study was to evaluate the formation during the disinfection processes of some toxic compounds that could explain the genotoxic effects of drinking waters. Differently treated waters were concentrated by solid-phase adsorption on silica C(18) columns and toxicity was assessed in a line of human hepatoma cells (HepG2), a metabolically competent cellular line very useful for human risk evaluation. The seasonal variability of the physical-chemical water characteristics (AOX, UV 254 nm, potential formation of THM, pH and temperature) made indispensable experimentation with water samples taken during the various seasons. Autumn waters cause greater toxicity compared to those of other seasons, in particular dilution of the concentrate at 0.5l equivalent of disinfected waters with chlorine dioxide and peracetic acid causes a 55% reduction in cellular vitality while the cellular vitality is over 80% with the all other water concentrates. Moreover it is very interesting underline that non-cytotoxic quantities of the autumnal water concentrates cause, after 2h treatment, a decrease in GSH and a statistically significant increase in oxygen radicals, while after prolonged treatment (24h) cause a GSH increase, without variations in the oxygen radical content. This phenomenon could be interpreted as the cellular

  16. Características epidemiológicas de la cirrosis hepática en el Hospital Civil de Guadalajara

    Directory of Open Access Journals (Sweden)

    CAMPOLLO OCTAVIO

    1997-01-01

    Full Text Available Objetivo. Estudiar prospectivamente las características demográficas y epidemiológicas de los pacientes con cirrosis hepática en el Hospital Civil de Guadalajara en un periodo de un año. Material y métodos. Se estudiaron 157 pacientes (48 mujeres y 109 hombres, de los servicios de Medicina Interna, Gastroenterología y Clínica de Hígado con diagnóstico de cirrosis hepática el cual se hizo con base en la información clínica, bioquímica o histopatológica; asimismo, se les aplicó un cuestionario especializado en enfermedades hepáticas. Resultados. La principal causa de la cirrosis fue el alcoholismo (38% en mujeres y 95% en hombres, seguida de la etiología viral. Las bebidas más frecuentes fueron el tequila y el alcohol de 96° G.L. El grado de insuficiencia hepática más frecuentemente observado fue el de Child-Pugh "B" en mujeres y "C" en los hombres. Las complicaciones más frecuentes fueron hemorragia de tubo digestivo, ascitis y encefalopatía hepática. Se observaron diferencias en varias características relacionadas con el sexo de los pacientes. Conclusiones. El alcoholismo fue la primera causa de cirrosis hepática. En mujeres la segunda causa fue la viral (16.7%. Se propone un comité nacional de vigilancia de enfermedades del hígado, para generar una información más completa y detallada acerca de la epidemiología de la cirrosis hepática.

  17. Korean Byungkyul - Citrus platymamma Hort.et Tanaka flavonoids induces cell cycle arrest and apoptosis, regulating MMP protein expression in Hep3B hepatocellular carcinoma cells.

    Science.gov (United States)

    Hong, Gyeong Eun; Lee, Ho Jeong; Kim, Jin A; Yumnam, Silvia; Raha, Suchismita; Venkatarame Gowda Saralamma, Venu; Heo, Jeong Doo; Lee, Sang Joon; Kim, Eun Hee; Won, Chun Kil; Kim, Gon Sup

    2017-02-01

    Citrus platymamma Hort.et Tanaka is an indigenous fruit of Jeju island in Korea. In this study the bioactivity of C. platymamma flavonoids were evaluated on human hepatoma Hep3B cell lines. Eleven flavonoids were identified from the peels of C. platymamma Hort.et Tanaka through high-performance liquid chromatography-Tandem mass spectrometry and the anticancer effect of these C. platymamma flavonoids on human hepatoma Hep3B were studied. Chromatin condensation was observed in Hep3B cells treated with C. platymamma flavonoids. DNA fragmentation was confirmed through agarose gel electrophoresis and TUNEL assay. An increase in the total apoptotic cells and G2/M cell cycle arrest with decreased protein expression of CDC25C, CDK1, cyclin B1 and p21 were observed in Hep3B cells treated with flavonoids of C. platymamma. Further, protein expression of Bcl-XL, Bax, caspase-3 and -9 were also modulated by C. platymamma flavonoids treatment indicating that cell death is through intrinsic apoptotic pathway. Moreover, C. platymamma flavonoids also regulated the phosphorylation of MAPKs, PI3K, and Akt in Hep3B cells. Relevant to inhibiting metastasis, C. platymamma treatment reduced wound closure of Hep3B cells and the protein expression of matrix metalloproteinase-2 and -9 were reduced in C. platymamma treated cells. The results show that C. platymamma flavonoids induce cell cycle arrest and apoptosis following activation of MAPKs and suppression of PI3K/Akt pathway which eventually inhibits cell migration in Hep3B cells. The finding provides evidence on biochemical activities of C. platymamma Hort.et Tanaka, which would be an essential agent for hepatocellular carcinoma (HCC) treatment.

  18. Apigenin suppresses GLUT-1 and p-AKT expression to enhance the chemosensitivity to cisplatin of laryngeal carcinoma Hep-2 cells: an in vitro study.

    Science.gov (United States)

    Xu, Ying-Ying; Wu, Ting-Ting; Zhou, Shui-Hong; Bao, Yang-Yang; Wang, Qin-Ying; Fan, Jun; Huang, Ya-Ping

    2014-01-01

    Glucose transporter-1 (GLUT-1) and PI3K/Akt are known to be closely involved in resistance to chemotherapy. Co-targeted therapy reducing GLUT-1 expression and PI3K/Akt pathway activity may overcome the chemoresistance of human cancers. Apigenin may inhibit the expression of GLUT-1 and the PI3K/Akt pathway. We hypothesized that over-expression of GLUT-1 and p-Akt was associated with the resistance to cisplatin of laryngeal carcinoma Hep-2 cells. We explored whether apigenin inhibited GLUT-1 and p-Akt, resulting in sensitization of laryngeal carcinoma Hep-2 cells to cisplatin. Real-time RT-PCR and Western blotting confirmed the presence of GLUT-1 mRNA, and GLUT-1 and p-Akt proteins in Hep-2 cells. We found that resistance or insensitivity of Hep-2 cells to cisplatin might be associated with such expression. Apigenin markedly enhanced the cisplatin-induced suppression of Hep-2 cell growth. This effect was concentration- and time-dependent. Thus apigenin may significantly reduce the levels of GLUT-1 mRNA, and GLUT-1 and p-Akt proteins, in cisplatin-treated Hep-2 cells, in a concentration- and time-dependent manner. To conclude, overexpression of GLUT-1 mRNA may be associated with the resistance to cisplatin of laryngeal carcinoma Hep-2 cells. Apigenin may enhance the sensitivity to cisplatin of laryngeal carcinoma cells via inhibition of GLUT-1 and p-Akt expression.

  19. Activation of human stearoyl-coenzyme A desaturase 1 contributes to the lipogenic effect of PXR in HepG2 cells.

    Directory of Open Access Journals (Sweden)

    Jun Zhang

    Full Text Available The pregnane X receptor (PXR was previously known as a xenobiotic receptor. Several recent studies suggested that PXR also played an important role in lipid homeostasis but the underlying mechanism remains to be clearly defined. In this study, we found that rifampicin, an agonist of human PXR, induced lipid accumulation in HepG2 cells. Lipid analysis showed the total cholesterol level increased. However, the free cholesterol and triglyceride levels were not changed. Treatment of HepG2 cells with rifampicin induced the expression of the free fatty acid transporter CD36 and ABCG1, as well as several lipogenic enzymes, including stearoyl-CoA desaturase-1 (SCD1, long chain free fatty acid elongase (FAE, and lecithin-cholesterol acyltransferase (LCAT, while the expression of acyl:cholesterol acetyltransferase(ACAT1 was not affected. Moreover, in PXR over-expressing HepG2 cells (HepG2-PXR, the SCD1 expression was significantly higher than in HepG2-Vector cells, even in the absence of rifampicin. Down-regulation of PXR by shRNA abolished the rifampicin-induced SCD1 gene expression in HepG2 cells. Promoter analysis showed that the human SCD1 gene promoter is activated by PXR and a novel DR-7 type PXR response element (PXRE response element was located at -338 bp of the SCD1 gene promoter. Taken together, these results indicated that PXR activation promoted lipid synthesis in HepG2 cells and SCD1 is a novel PXR target gene.

  20. A fully liquid DTaP-IPV-Hep B-PRP-T hexavalent vaccine for primary and booster vaccination of healthy Mexican children.

    Science.gov (United States)

    Aquino, Amalia Guadalupe Becerra; Brito, Maricruz Gutiérrez; Doniz, Carlos E Aranza; Herrera, Juan Francisco Galán; Macias, Mercedes; Zambrano, Betzana; Plennevaux, Eric; Santos-Lima, Eduardo

    2012-10-05

    To evaluate an investigational, fully liquid hexavalent diphtheria-tetanus-acellular pertussis-inactivated poliovirus-hepatitis B-Haemophilus influenzae type b (DTaP-IPV-Hep B-PRP-T: Hexaxim™) vaccine for primary and booster vaccination of healthy children in Mexico. Infants (N=1189) were randomized to receive one of three lots of the DTaP-IPV-Hep B-PRP-T vaccine or a licensed hexavalent control vaccine (Infanrix™ hexa) for primary vaccination at 2, 4 and 6 months. All participants who completed the primary series and agreed to participate in the booster part of the study received a dose of the investigational vaccine at 15-18 months of age. Validated serological assays and parental reports were used to assess immunogenicity and safety, respectively. Post-primary vaccination, ≥95.8% of participants in both the DTaP-IPV-Hep B-PRP-T and control groups were seroprotected (SP) against diphtheria, tetanus, poliovirus, hepatitis B and PRP, or had seroconverted (SC) to the pertussis toxin (PT) and filamentous hemagglutinin (FHA) pertussis antigens. The SP/SC rates induced by the three DTaP-IPV-Hep B-PRP-T lots were equivalent. No differences in SP/SC rates were observed between the pooled lots of investigational vaccine and the control vaccine. Antibody persistence at 15-18 months was comparable between groups, with strong increases in all antibody concentrations post-DTaP-IPV-Hep B-PRP-T booster. Both vaccines were well tolerated for primary vaccination, as was the booster dose of DTaP-IPV-Hep B-PRP-T. These study findings confirm the suitability of the combined, fully liquid DTaP-IPV-Hep B-PRP-T vaccine for inclusion in routine childhood vaccination schedules. Copyright © 2012 Elsevier Ltd. All rights reserved.

  1. Ressecção hepática robótica. Relato de experiência pioneira na América Latina First robotic-assisted laparoscopic liver resection in Latin America

    Directory of Open Access Journals (Sweden)

    Marcel Autran C. Machado

    2009-03-01

    Full Text Available Graças ao melhor conhecimento da anatomia segmentar do fígado e desenvolvimento de novas técnicas, houve aumento no número de indicações de hepatectomias. O desenvolvimento da cirurgia minimamente invasiva ocorreu paralelamente e o aumento da experiência, aliado ao desenvolvimento de novos instrumentais, resultaram no crescimento exponencial das ressecções hepáticas videolaparoscópicas. A abordagem laparoscópica pode tornar viável a ressecção hepática em pacientes cirróticos com hipertensão portal que não tolerariam este mesmo procedimento por via laparotômica. A cirurgia robótica surgiu nos últimos anos como a última fronteira de desenvolvimento técnico aplicado à videocirurgia. O presente trabalho descreve a experiência pioneira de ressecção hepática totalmente com o uso de robótica na América Latina, em paciente com carcinoma hepatocelular e cirrose hepática. A hepatectomia laparoscópica com o uso do sistema robótico Da Vinci permite refinamentos técnicos graças à visualização tridimensional do campo cirúrgico e utilização de instrumentais precisos e com grande amplitude de movimentação que simulam os movimentos da mão humana.The surgical robotic system is superior to traditional laparoscopy in regards to 3-dimensional images and better instrumentations. Robotic surgery for hepatic resection has not yet been extensively reported. The aim of this paper is to report the first known case of liver resection with use of a computer-assisted, or robotic, surgical device in Latin America. A 72-year-old male with cryptogenic liver cirrhosis and hepatocellular carcinoma was referred for surgical treatment. Preoperative clinical evaluation and laboratory data disclosed a Child-Pugh class A patient. Magnetic resonance imaging showed a 2.2 cm tumor in segment 5. Liver size was decreased and there were signs of portal hypertension, such as splenomegaly and enlarged portal vein collaterals. Preoperative upper

  2. Tumores malignos de novo en el trasplante hepático experiencia de un centro en la Argentina

    Directory of Open Access Journals (Sweden)

    Florencia Antinucci

    2015-02-01

    Full Text Available El objetivo del presente trabajo ha sido evaluar la incidencia y las características clínicas de los tumores aparecidos de novo en los pacientes sometidos a trasplante hepático, como así también su supervivencia. Para ello, analizamos en forma retrospectiva los 168 trasplantes hepáticos realizados en 159 pacientes adultos en el período mayo 2006 hasta mayo 2014, encontrando una incidencia de neoplasia de novo de 7.5% (n = 12. La edad media en el momento del diagnóstico fue de 63 ± 7 años. Las neoplasias más frecuentes fueron las de piel no melanoma y adenocarcinomas. El 50% de las neoplasias se desarrollaron en el segundo y tercer año postrasplante. El tipo de inmunosupresión no influyó en el tipo de tumor; sin embargo, debemos destacar que la mayor parte de los pacientes recibieron tacrolimus, micofenolato y/o corticoides. El tiempo medio de seguimiento tras el diagnóstico del tumor fue 25 ± 29 meses (0-76, y la tasa de mortalidad fue de un 41% (5/12 pacientes IC95%,15-72.La supervivencia global luego del trasplante a 1 y 5 años, calculada por análisis de Kaplan-Meier, fue de 83 y 55%, respectivamente. Los tumores de novo son frecuentes luego del trasplante hepático y presentan un patrón evolutivo diferente al de la población general. Teniendo en cuenta esta evolución más agresiva, es fundamental el seguimiento periódico en estos pacientes para realizar un diagnóstico lo más precoz posible.

  3. BC047440 antisense eukaryotic expression vectors inhibited HepG2 cell proliferation and suppressed xenograft tumorigenicity

    International Nuclear Information System (INIS)

    Lu, Zheng; Ping, Liang; JianBo, Zhou; XiaoBing, Huang; Yu, Wen; Zheng, Wang; Jing, Li

    2012-01-01

    The biological functions of the BC047440 gene highly expressed by hepatocellular carcinoma (HCC) are unknown. The objective of this study was to reconstruct antisense eukaryotic expression vectors of the gene for inhibiting HepG 2 cell proliferation and suppressing their xenograft tumorigenicity. The full-length BC047440 cDNA was cloned from human primary HCC by RT-PCR. BC047440 gene fragments were ligated with pMD18-T simple vectors and subsequent pcDNA3.1(+) plasmids to construct the recombinant antisense eukaryotic vector pcDNA3.1(+)BC047440AS. The endogenous BC047440 mRNA abundance in target gene-transfected, vector-transfected and naive HepG 2 cells was semiquantitatively analyzed by RT-PCR and cell proliferation was measured by the MTT assay. Cell cycle distribution and apoptosis were profiled by flow cytometry. The in vivo xenograft experiment was performed on nude mice to examine the effects of antisense vector on tumorigenicity. BC047440 cDNA fragments were reversely inserted into pcDNA3.1(+) plasmids. The antisense vector significantly reduced the endogenous BC047440 mRNA abundance by 41% in HepG 2 cells and inhibited their proliferation in vitro (P < 0.01). More cells were arrested by the antisense vector at the G 1 phase in an apoptosis-independent manner (P = 0.014). Additionally, transfection with pcDNA3.1(+) BC047440AS significantly reduced the xenograft tumorigenicity in nude mice. As a novel cell cycle regulator associated with HCC, the BC047440 gene was involved in cell proliferation in vitro and xenograft tumorigenicity in vivo through apoptosis-independent mechanisms

  4. Increased glucose metabolism and alpha-glucosidase inhibition in Cordyceps militaris water extract-treated HepG2 cells

    Science.gov (United States)

    Kim, Dae Jung; Kang, Yun Hwan; Kim, Kyoung Kon; Kim, Tae Woo; Park, Jae Bong

    2017-01-01

    BACKGROUND/OBJECTIVES Recent living condition improvements, changes in dietary habits, and reductions in physical activity are contributing to an increase in metabolic syndrome symptoms including diabetes and obesity. Through such societal developments, humankind is continuously exposed to metabolic diseases such as diabetes, and the number of the victims is increasing. This study investigated Cordyceps militaris water extract (CMW)-induced glucose uptake in HepG2 cells and the effect of CMW treatment on glucose metabolism. MATERIALS/METHODS Colorimetric assay kits were used to determine the glucokinase (GK) and pyruvate dehydrogenase (PDH) activities, glucose uptake, and glycogen content. Either RT-PCR or western blot analysis was performed for quantitation of glucose transporter 2 (GLUT2), hepatocyte nuclear factor 1 alpha (HNF-1α), phosphatidylinositol 3-kinase (PI3k), protein kinase B (Akt), phosphorylated AMP-activated protein kinase (pAMPK), phosphoenolpyruvate carboxykinase, GK, PDH, and glycogen synthase kinase 3 beta (GSK-3β) expression levels. The α-glucosidase inhibitory activities of acarbose and CMW were evaluated by absorbance measurement. RESULTS CMW induced glucose uptake in HepG2 cells by increasing GLUT2 through HNF-1α expression stimulation. Glucose in the cells increased the CMW-induced phosphorylation of AMPK. In turn, glycolysis was stimulated, and glyconeogenesis was inhibited. Furthermore, by studying the mechanism of action of PI3k, Akt, and GSK-3β, and measuring glycogen content, the study confirmed that the glucose was stored in the liver as glycogen. Finally, CMW resulted in a higher level of α-glucosidase inhibitory activity than that from acarbose. CONCLUSION CMW induced the uptake of glucose into HepG2 cells, as well, it induced metabolism of the absorbed glucose. It is concluded that CMW is a candidate or potential use in diabetes prevention and treatment. PMID:28584574

  5. Differential gene expression in human hepatocellular carcinoma Hep3B cells induced by apoptosis-related gene BNIPL-2.

    Science.gov (United States)

    Xie, Li; Qin, Wen-Xin; He, Xiang-Huo; Shu, Hui-Qun; Yao, Gen-Fu; Wan, Da-Fang; Gu, Jian-Ren

    2004-05-01

    Bcl-2/adenovirus E1B 19 ku interacting protein 2-like (BNIPL-2) is a novel protein recently identified in our laboratory. BNIPL-2 is homologous to human BNIP-2, a potentially proapoptotic protein, and can interact with Bcl-2 and Cdc42GAP and promote apoptosis in BEL-7402 cells. Here we report the gene-expression profile regulated by BNIPL-2 in human hepatocarcinoma Hep3B cells and the analysis of its potential roles in cell apoptosis. BNIPL-2 was overexpressed in Hep3B cells using tetracycline inducible or Tet-on system. Screened by Western blot, the cells with low background and high induction fold of BNIPL-2 were obtained. We performed Atlas human cDNA expression array hybridization on these cells and analyzed the data with Quantarray software to identify BNIPL-2-regulated genes and their expression profile. RT-PCR was used to confirm the altered expression level of part of genes identified by the Atlas array hybridization. Fifteen of 588 genes spotted on the Atlas membrane showed altered expression levels in BNIPL-2-transfected Hep3B-Tet-on cells, in which 8 genes involved in cell apoptosis or growth inhibition were up-regulated and 7 genes involved in cellular proliferation were down-regulated following overexpression of BNIPL-2. cDNA array is a powerful tool to explore gene expression profiles under inducible conditions. The data obtained using the cDNA expression microarray technology indicates that BNIPL-2 may play its roles in apoptosis through regulating the expression of genes associated with cell apoptosis, growth inhibition and cell proliferation.

  6. Free radical generation from an aniline derivative in HepG2 cells: a possible captodative effect.

    Science.gov (United States)

    Horinouchi, Yuya; Summers, Fiona A; Ehrenshaft, Marilyn; Mason, Ronald P

    2015-01-01

    Xenobiotic metabolism can induce the generation of protein radicals, which are believed to play an important role in the toxicity of chemicals and drugs. It is therefore important to identify chemical structures capable of inducing macromolecular free radical formation in living cells. In this study, we evaluated the ability of four structurally related environmental chemicals, aniline, nitrosobenzene, N,N-dimethylaniline, and N,N-dimethyl-4-nitrosoaniline (DMNA), to induce free radicals and cellular damage in the hepatoma cell line HepG2. Cytotoxicity was assessed using lactate dehydrogenase assays, and morphological changes were observed using phase contrast microscopy. Protein free radicals were detected by immuno-spin trapping using in-cell western experiments and confocal microscopy to determine the subcellular locale of free radical generation. DMNA induced free radical generation, lactate dehydrogenase release, and morphological changes in HepG2 cells, whereas aniline, nitrosobenzene, N,N-dimethylaniline did not. Confocal microscopy showed that DMNA induced free radical generation mainly in the cytosol. Preincubation of HepG2 cells with N-acetylcysteine and 2,2'-dipyridyl significantly prevented free radical generation on subsequent incubation with DMNA, whereas preincubation with apocynin and dimethyl sulfoxide had no effect. These results suggest that DMNA is metabolized to reactive free radicals capable of generating protein radicals which may play a critical role in DMNA toxicity. We propose that the captodative effect, the combined action of the electron-releasing dimethylamine substituent, and the electron-withdrawing nitroso substituent, leads to a thermodynamically stabilized radical, facilitating enhanced protein radical formation by DMNA. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. Regulation of glucose transporter protein-1 and vascular endothelial growth factor by hypoxia inducible factor 1α under hypoxic conditions in Hep-2 human cells.

    Science.gov (United States)

    Xu, Ou; Li, Xiaoming; Qu, Yongtao; Liu, Shuang; An, Jie; Wang, Maoxin; Sun, Qingjia; Zhang, Wen; Lu, Xiuying; Pi, Lihong; Zhang, Min; Shen, Yupeng

    2012-12-01

    The present study evaluated the regulation of glucose transporter protein-1 (Glut-1) and vascular endothelial growth factor (VEGF) by hypoxia inducible factor 1α (HIF-1α) under hypoxic conditions in Hep-2 human cells to explore the feasibility of these three genes as tumor markers. Hep-2 cells were cultured under hypoxic and normoxic conditions for 6, 12, 24, 36 and 48 h. The proliferation of Hep-2 cells was evaluated using an MTT assay. The protein and mRNA expression levels of HIF-1α, Glut-1 and VEGF were detected using the S-P immunocytochemical method, western blotting and reverse transcription polymerase chain reaction (RT-PCR). The results revealed that the expression levels of HIF-1α, Glut-1 and VEGF protein in Hep-2 cells were significantly elevated under hypoxic conditions compared with those under normoxic conditions over 36 h. Under hypoxic conditions, mRNA levels of HIF-1α were stable, while mRNA levels of Glut-1 and VEGF changed over time. In conclusion, Glut-1 and VEGF were upregulated by HIF-1α under hypoxic conditions in a time-dependent manner in Hep-2 cells and their co-expression serves as a tumor marker.

  8. [Adenovirus-mediated killing of hepatocellular carcinoma HepG2 cells by heterogeneous fusion gene NT4p53(N15)Ant].

    Science.gov (United States)

    Li, Yue-ping; Qiu, Shu-dong; Song, Li-ping; Wang, Quan-ying; Yang, Guang-xiao

    2007-07-01

    OBJECTIVE To construct a recombinant adenovirus Ad.NT4p53(N15)Ant and explore its cytotoxic effect against hepatocellular carcinoma HepG2 cells in vitro. The recombinant adenovirus containing the fusion gene of neurotrophin 4 (NT4)signal peptide, N-terminal residues (12-26) of p53 and 17 amino acid Drosophila homeobox protein Antennapedia (Ant) was constructed by gene cloning protocol. The effect of this fusion gene on HepG2 cells was evaluated by MTT assay, PI staining and flow cytometry. The fusion gene Ad.NT4p53(N15)Ant was successfully constructed, as verified by restriction endonuclease digestion and PCR. Ad.NT4p53(N15)Ant could strongly suppress the growth of HepG2 cells (with a growth inhibition rate of 63.3% 48 h after infection) without affecting NIH-3T3 cells. Flow cytometry showed that Ad.NT4p53(N15)Ant could induce obvious apoptosis of HepG2 cells. The recombinant adenovirus containing NT4p53(N15)Ant fusion gene can inhibit the growth the of HepG2 cells in vitro partially by inducing cell apoptosis.

  9. CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation

    Directory of Open Access Journals (Sweden)

    Shi Lixin

    2011-01-01

    Full Text Available Abstract Cadmium telluride quantum dots (Cdte QDs have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

  10. Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells

    Directory of Open Access Journals (Sweden)

    Zhang Chong

    2011-05-01

    Full Text Available Abstract Background To investigate the effect of HBV on the proliferative ability of host cells and explore the potential mechanism. Methods MTT, colony formation assay and tumourigenicity in nude mice were performed to investigate the effect of HBV on the proliferative capability of host cells. In order to explore the potential mechanism, cell cycle and apoptosis were analysed. The cell cycle genes controlling the G1/S phase transition were detected by immunohistochemistry, westernblot and RT-PCR. Results HepG2.2.15 cells showed decreased proliferation ability compared to HepG2 cells. G1 phase arrest was the main cause but was not associated with apoptosis. p53, p21 and total retinoblastoma (Rb were determined to be up-regulated, whereas cyclinE was down-regulated at both the protein and mRNA levels in HepG2.2.15 cells. The phosphorylated Rb in HepG2.2.15 cells was decreased. Conclusions Our results suggested that HBV inhibited the capability of proliferation of HepG2.2.15 cells by regulating cell cycle genes expression and inducing G1 arrest.

  11. Liver cirrhosis and hepatic stellate cells Cirrose hepática e células estreladas do figado

    Directory of Open Access Journals (Sweden)

    Daniel Ferracioli Brandão

    2006-01-01

    Full Text Available The cirrhosis represents the final stage of several chronic hepatic diseases and it is characterized by the presence of fibrosis and morphologic conversion from the normal hepatic architecture into structurally abnormal nodules. In the evolution of the disease there is loss of the normal vascular relationship and portal hypertension. There are also regenerative hepatocelular alterations that become more prominent with the progression of the disease. The liver transplantation continues to be the only therapeutic option in cases of disease in terminal phase. The hepatic stellate cells (HSC are perisinusoidal cells that store vitamin A and produce growth factors, citocins, prostaglandins and other bioactive substances. They can suffer an activation process that convert them to cells with a phenotype similar to myofibroblasts. When activated, they present increased capacity of proliferation, mobility, contractility and synthesis of collagen and other components of extracelular matrix. They possess cytoplasmic processes adhered to sinusoids and can affect the sinusoidal blood flow. HSC are important in pathogenesis of fibrosis and portal hypertension.A cirrose representa o estágio final de diversas doenças hepáticas crônicas e é caracterizada pela presença de fibrose e conversão da arquitetura hepática normal em nódulos estruturalmente anormais. Na evolução da doença ocorre perda da relação vascular normal e hipertensão portal. Há também alterações regenerativas hepatocelulares que se tornam mais proeminentes com a progressão da doença. O transplante hepático permanece como a única opção terapêutica nos casos de doença em fase terminal. As células estreladas hepáticas (CEH são células perisinusoidais que armazenam vitamina A e produzem fatores de crescimento, citocinas, prostaglandinas e outras substâncias bioativas. Podem sofrer um processo de ativação para um fenótipo semelhante a miofibroblastos. Quando ativadas

  12. Hypocholesterolemic mechanism of phenolics-enriched extract from Moringa oleifera leaves in HepG2 cell lines

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    Peera Tabboon

    2016-04-01

    Full Text Available Previous studies have demonstrated the hypolipidemic activity of Moringa oleifera (MO leaves via lowering serum levels of cholesterol, but the mechanism of action is unknown. In this study, we demonstrated the hypocholesterolemic mechanism of a phenolics-enriched extract of Moringa oleifera leaf (PMO in HepG2 cells. When compared to the control treatment, PMO significantly decreased total intracellular cholesterol, inhibited the activity of HMG CoA reductase in a dosedependent manner and enhanced LDL receptor binding activity. Moreover, PMO also significantly increased the genetic expressions of HMG CoA reductase and LDL receptor.

  13. Evaluación del serodiagnóstico en el absceso hepático amebiano

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    Luis Carlos Orozco

    1997-12-01

    Full Text Available El presente estudio fue diseñado para evaluar la eficacia del diagnóstico serológico del absceso hepático amibiano (AHA. Se utilizó una prueba de ELISA para la detección en suero de IgG especifica, utilizando una fase sólida con una alta capacidad de adherencia. Se estudiaron 147 personas; 22 pacientes con sospecha clínica y ultrasonográfica de AHA, 30 individuos completamente sanos, 9 portadores asintomáticos de E. histolytica, 35 con colitis amebiana pasada, 35 con otras parasitosis intestinales, 9 con otras patologías hepáticas y 6 con colitis amebiana presente. El rendimiento global de la prueba fue analizado por medio de las curvas del receptor-operador y del área bajo la curva. Se determinó que existe una diferencia significativa en la densidad óptica (DO de los siete grupos estudiados (Kruskal-Wallis entre todos los grupos: p=0,0001; esta diferencia no existe cuando, al comparar, se elimina el grupo 1 del análisis (Kruskal-Wallis exceptuando el grupo 1: p=0,8203. El área bajo la curva ROC fue igual a 0,9941, dato muy cercano al ideal que es 1. Se recomienda la utilización de esta fase sólida teniendo en cuenta la importancia de la fase de bloqueo con albúmina de huevo. Se determinó que, a pesar de estar en una zona donde el diagnóstico de amebiasis es frecuente, las infecciones intestinales por esta ameba no presentan niveles detectables de IgG especifica contra E. histolytica por esta prueba. Sin embargo, es importante ampliar la cantidad de personas estudiadas en estos grupos, principalmente, en el grupo de pacientes con otras patologías hepáticas puesto que este grupo incluye las entidades clínicas con las que realmente se debe hacer el diagnóstico diferencial de (AHA (absceso hepático piógeno (AHP, principalmente para poder realizar la última fase de la evaluación. El estudio de este grupo nos ayudaría a obtener valores reales de sensibilidad, especificidad y valores predictivos y nos darían una

  14. FPGA-based fast pipeline-parameterized-sorter implementation for first level trigger systems in HEP experiments

    CERN Document Server

    Pozniak, Krzysztof T

    2004-01-01

    The paper describes a behavioral model of fast, pipeline sorter dedicated to electronic triggering applications in the experiments of high energy physics (HEP). The sorter was implemented in FPGA for the RPC Muon Detector of CMS experiment (LHC accelerator, CERN) and for Backing Calorimeter (BAC) in ZEUS experiment (HERA accelerator, DESY) . A general principle of the applied sorting algorithm was presented. The implementation results were debated in detail for chosen FPGA chips by ALTERA and XILINX manufactures. The realization costs have been calculated as function of system parameters.

  15. Synthesis, characterization and anticancer effect of the ruthenium (II) polypyridyl complexes on HepG2 cells.

    Science.gov (United States)

    Wan, Dan; Lai, Shang-Hai; Yang, Hui-Hui; Tang, Bing; Zhang, Cheng; Yin, Hui; Zeng, Chuan-Chuan; Liu, Yun-Jun

    2016-12-01

    As one of the major cell regulated center, mitochondria are closely associated with cell proliferation, apoptosis of tumor cell. In this work, four new ruthenium (II) polypyridyl complexes [Ru(bpy) 2 (FTTP)](ClO 4 ) 2 (1) (FTTP=11-(3-fluoro-naphthalen-2-yloxy)-4,5,9,14-tetraaza-benzo[b]triphenylene, bpy=2,2'-bipyridine), [Ru(phen) 2 (FTTP)](ClO 4 ) 2 (2) (phen=1,10-phenanthroline), [Ru(bpy) 2 (PTTP)](ClO 4 ) 2 (3) (PTTP=2-phenoxy-1,4,8,9-tetraazatriphenylene) and [Ru(phen) 2 (PTTP)](ClO 4 ) 2 (4) were synthesized and characterized by elemental analysis, ESI-MS, 1 H NMR and 13 C NMR. The cytotoxic activity, ability of inhibiting cell invasion, cell cycle arrest and apoptosis-inducing mechanism of these Ru(II) complexes have been investigated in detail by MTT (3-(4,5-dimethylthiazole)-2,5-diphenyltetrazolium bromide) method, invasion assay, comet assay as well as western blotting techniques. Notably, complexes 1-4 displayed high cytotoxic activity against liver carcinoma HepG2 cells and the IC 50 values of complexes 1-4 against HepG2 cells are 10.4±1.2, 9.3±0.6, 29.1±1.5 and 5.6±1.2μM, respectively. The comet assay showed that the complexes can induce DNA damage. The acridine orange (AO) and ethidium bromide (EB) staining method indicated that the complexes can cause apoptosis in HepG2 cells. Further studies showed that complexes 1-4 caused cell cycle arrest at G0/G1 phase and induced HepG2 cells apoptosis through a ROS-mediated mitochondrial dysfunction pathway, which involved an increase in the levels of reactive oxygen species (ROS), a decrease in the mitochondrial membrane potential, activation of caspases and Bcl-2 family proteins. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. In vitro cytotoxicity and induction of apoptosis by silica nanoparticles in human HepG2 hepatoma cells

    Directory of Open Access Journals (Sweden)

    Lu X

    2011-09-01

    Full Text Available Xun Lu2,3, Jiangchao Qian1, Huanjun Zhou2,3, Qi Gan2,3, Wei Tang1, Jingxiong Lu3, Yuan Yuan1,2, Changsheng Liu1–31State Key Laboratory of Bioreactor Engineering, 2Key Laboratory for Ultrafine Materials of Ministry of Education, 3Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai, People’s Republic of ChinaBackground: Silica nanoparticles have been discovered to exert cytotoxicity and induce apoptosis in normal human cells. However, until now, few studies have investigated the cytotoxicity of silica nanoparticles in tumor cells.Methods: This study investigated the cytotoxicity of 7–50 nm silica nanoparticles in human HepG2 hepatoma cells, using normal human L-02 hepatocytes as a control. Cell nucleus morphology changes, cellular uptake, and expression of procaspase-9, p53, Bcl-2, and Bax, as well as the activity of caspase-3, and intracellular reactive oxygen species and glutathione levels in the silica nanoparticle-treated cells, were analyzed.Results: The antitumor activity of the silica nanoparticles was closely related to particle size, and the antiproliferation activity decreased in the order of 20 nm > 7 nm > 50 nm. The silica nanoparticles were also cytotoxic in a dose- and time-dependent manner. However, the silica nanoparticles showed only slight toxicity in the L-02 control cells, Moreover, in HepG2 cells, oxidative stress and apoptosis were induced after exposure to 7–20 nm silica nanoparticles. Expression of p53 and caspase-3 increased, and expression of Bcl-2 and procaspase-9 decreased in a dose-dependent manner, whereas the expression of Bax was not significantly changed.Conclusion: A mitochondrial-dependent pathway triggered by oxidative stress mediated by reactive oxygen species may be involved in apoptosis induced by silica nanoparticles, and hence cytotoxicity in human HepG2 hepatic cancer cells.Keywords: silica nanoparticles

  17. Comparative cytotoxicity of nanosilver in human liver HepG2 and colon Caco2 cells in culture.

    Science.gov (United States)

    Sahu, Saura C; Zheng, Jiwen; Graham, Lesley; Chen, Lynn; Ihrie, John; Yourick, Jeffrey J; Sprando, Robert L

    2014-11-01

    The use of silver nanoparticles in food, food contact materials, dietary supplements and cosmetics has increased significantly owing to their antibacterial and antifungal properties. As a consequence, the need for validated rapid screening methods to assess their toxicity is necessary to ensure consumer safety. This study evaluated two widely used in vitro cell culture models, human liver HepG2 cells and human colon Caco2 cells, as tools for assessing the potential cytotoxicity of food- and cosmetic-related nanoparticles. The two cell culture models were utilized to compare the potential cytotoxicity of 20-nm silver. The average size of the silver nanoparticle determined by our transmission electron microscopy (TEM) analysis was 20.4 nm. The dynamic light scattering (DLS) analysis showed no large agglomeration of the silver nanoparticles. The concentration of the 20-nm silver solution determined by our inductively coupled plasma-mass spectrometry (ICP-MS) analysis was 0.962 mg ml(-1) . Our ICP-MS and TEM analysis demonstrated the uptake of 20-nm silver by both HepG2 and Caco2 cells. Cytotoxicity, determined by the Alamar Blue reduction assay, was evaluated in the nanosilver concentration range of 0.1 to 20 µg ml(-1) . Significant concentration-dependent cytotoxicity of the nanosilver in HepG2 cells was observed in the concentration range of 1 to 20 µg ml(-1) and at a higher concentration range of 10 to 20 µg ml(-1) in Caco2 cells compared with the vehicle control. A concentration-dependent decrease in dsDNA content was observed in both cell types exposed to nanosilver but not controls, suggesting an increase in DNA damage. The DNA damage was observed in the concentration range of 1 to 20 µg ml(-1) . Nanosilver-exposed HepG2 and Caco2 cells showed no cellular oxidative stress, determined by the dichlorofluorescein assay, compared with the vehicle control in the concentration range used in this study. A concentration-dependent decrease in

  18. Hidatidosis retroperitoneal secundaria a quiste hidatídico de localización hepática Retroperitoneal hydatidosis secondary to hepatic hydatid cyst

    Directory of Open Access Journals (Sweden)

    Katherina A Vizcaychipi

    2012-12-01

    Full Text Available La hidatidosis es una enfermedad de distribución mundial, producida por un platelminto parásito del género Echinococcus. El caso que se presenta corresponde a una paciente con una tumoración fluctuante en el espacio retroperitoneal lumbar, secundaria a un quiste hepático. El diagnóstico inicial de certeza fue dado por el hallazgo de ganchos rostelares de protoescólices en el líquido aspirado de un absceso. Este trabajo describe el cuadro clínico, el diagnóstico y el tratamiento médico-quirúrgico de esta paciente. Se analiza cómo la elaboración de un diagnóstico certero requiere de un análisis adecuado de los antecedentes epidemiológicos, las manifestaciones clínicas, los estudios de imágenes y las pruebas de laboratorio, ya que el conjunto de estos datos confirman el caso.Hydatid disease in a worldwide zoonosis. It is caused by a parasitic platyhelminth of the genus Echinococcus. We present a patient with a fluctuating lumbar tumor in the retroperitoneal space, secondary to a hepatic cyst. The initial diagnosis was made by identification of rostellar hooks from protoscoleces in the fluid aspirated from the abscess. We herein describe the clinical manifestations, diagnosis and medical and surgical treatment of this unusual case and conclude that the development of an accurate diagnosis required a proper analysis of the patient's epidemiological history, clinical manifestations, imaging studies and laboratory tests. A multidisciplinary approach and differential diagnosis is paramount to be able to establish a cause of the disease to deliver appropriate treatment.

  19. Using survey results regarding hepatitis B knowledge, community awareness and testing behavior among Asians to improve the San Francisco Hep B Free campaign.

    Science.gov (United States)

    Shiau, Rita; Bove, Fred; Henne, Jeff; Zola, Janet; Fang, Ted; Fernyak, Susan

    2012-04-01

    Asians are disproportionately affected by chronic hepatitis B (HBV) infection and its fatal consequences. The Hep B Free campaign was launched to eliminate HBV in San Francisco by increasing awareness, testing, vaccination and linkage to care. The campaign conducted 306 street intercept and telephone interviews of San Francisco Asians to assess current levels of HBV knowledge, testing behaviors and effectiveness of existing campaign media materials. One-third of respondents ranked HBV as a key health issue in the Asian community, second to diabetes. General HBV awareness is high (85%); however, a majority could not name an effective prevention method. Sixty percent reported having been tested for HBV; provider recommendation was the most often cited reason for testing. Respondents reported a high level of trust in their providers to correctly assess which health issues they may be at risk for developing and test accordingly, confirming that efforts to increase HBV testing among Asians must simultaneously mobilize the public to request testing and compel providers to test high-risk patients. Regarding community awareness, more than half reported hearing more about HBV recently; younger respondents were more likely to have encountered campaign materials and recall correct HBV facts. Assessment of specific campaign materials found that while upbeat images and taglines captured attention and destigmatized HBV, messages that emphasize the pervasiveness and deadly consequence of infection were more likely to drive respondents to seek education and testing. The campaign used survey results to focus efforts on more intensive provider outreach and to create messages for a new public outreach media campaign.

  20. Heparin conjugated quantum dots for in vitro imaging applications.

    Science.gov (United States)

    Maguire, Ciaran Manus; Mahfoud, Omar Kazem; Rakovich, Tatsiana; Gerard, Valerie Anne; Prina-Mello, Adriele; Gun'ko, Yurii; Volkov, Yuri

    2014-11-01

    In this work heparin-gelatine multi-layered cadmium telluride quantum dots (QDgel/hep) were synthesised using a novel 'one-pot' method. The QDs produced were characterised using various spectroscopic and physiochemical techniques. Suitable QDs were then selected and compared to thioglycolic acid stabilised quantum dots (QDTGA) and gelatine coated quantum dots (QDgel) for utilisation in in vitro imaging experiments on live and fixed permeabilised THP-1, A549 and Caco-2 cell lines. Exposure of live THP-1 cells to QDgel/hep resulted in localisation of the QDs to the nucleus of the cells. QDgel/hep show affinity for the nuclear compartment of fixed permeabilised THP-1 and A549 cells but remain confined to cytoplasm of fixed permeabilised Caco-2 cells. It is postulated that heparin binding to the CD11b receptor facilitates the internalisation of the QDs into the nucleus of THP-1 cells. In addition, the heparin layer may reduce the unfavourable thrombogenic nature of quantum dots observed in vivo. In this study, heparin conjugated quantum dots were found to have superior imaging properties compared to its native counterparts. The authors postulate that heparin binding to the CD11b receptor facilitates QD internalization to the nucleus, and the heparin layer may reduce the in vivo thrombogenic properties of quantum dots. Copyright © 2014 Elsevier Inc. All rights reserved.