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Sample records for spathelia excelsa rutaceae

  1. Chemical constituents of the stems of Spathelia excelsa (rutaceae) and activity against Aedes aegypti; Constituintes quimicos do caule de Spathelia excelsa (rutaceae) e atividade contra Aedes aegypti

    Energy Technology Data Exchange (ETDEWEB)

    Freitas, Aline Carvalho de; Lima, Maria da Paz [Instituto Nacional de Pesquisas da Amazonia (INPA), Manaus, AM (Brazil). Coordenacao de Pesquisas em Produtos Naturais], e-mail: mdapaz@inpa.gov.br; Ferreira, Antonio Gilberto [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica; Tadei, Wanderli Pedro; Pinto, Ana Cristina da Silva [Instituto Nacional de Pesquisas da Amazonia (INPA), Manaus, AM (Brazil). Lab. de Vetores de Malaria e Dengue

    2009-07-01

    Phytochemical investigation from the stems of Spathelia excelsa (Rutaceae) collected in Amazonas yielded deacetylspathelin (1), 7,8-dimethoxyflindersine (2), new glabretal-type triterpenoid 3{beta}-angeloyl-21,24-epoxy-7{alpha}, 21{alpha}, 23{alpha}, 25-tetrahydroxy-4{alpha}, 4{beta}, 8{beta}, 10{beta}-tetramethyl-25-dimethyl-14,18-cyclo-5{alpha}, 13{alpha}, 14{alpha}, 17{alpha}-cholestane (3), in addition to the known steroids s-sitosterol and stigmasterol. Their structures were established on the basis of spectral data. The compounds 1 and 3 were assayed on Aedes aegypti (larvicidal and adulticidal activities and compound 3 exhibited larvicidal properties with LC{sub 50} of 4,8 {mu}g/mL. (author)

  2. Chemical constituents of the stems of Spathelia excelsa (rutaceae) and activity against Aedes aegypti

    International Nuclear Information System (INIS)

    Freitas, Aline Carvalho de; Lima, Maria da Paz; Ferreira, Antonio Gilberto; Tadei, Wanderli Pedro; Pinto, Ana Cristina da Silva

    2009-01-01

    Phytochemical investigation from the stems of Spathelia excelsa (Rutaceae) collected in Amazonas yielded deacetylspathelin (1), 7,8-dimethoxyflindersine (2), new glabretal-type triterpenoid 3β-angeloyl-21,24-epoxy-7α, 21α, 23α, 25-tetrahydroxy-4α, 4β, 8β, 10β-tetramethyl-25-dimethyl-14,18-cyclo-5α, 13α, 14α, 17α-cholestane (3), in addition to the known steroids s-sitosterol and stigmasterol. Their structures were established on the basis of spectral data. The compounds 1 and 3 were assayed on Aedes aegypti (larvicidal and adulticidal activities and compound 3 exhibited larvicidal properties with LC 50 of 4,8 μg/mL. (author)

  3. Chemical constituents from the roots of Spathelia excelsa and their antiprotozoal activity

    Energy Technology Data Exchange (ETDEWEB)

    Moreira, Wagner A. dos Santos; Lima, Maria da Paz [Instituto Nacional de Pesquisas da Amazonia (IMPA), Manaus, AM (Brazil). Coordenacao de Pesquisas em Produtos Naturais], e-mail: mdapaz@inpa.gov.br; Ferreira, Antonio Gilberto [Universidade Federal de Sao Carlos (UFSCar), SP (Brazil). Dept. de Quimica; Ferreira, Izabel C. Piloto; Nakamura, Celso V. [Universidade Estadual de Maringa (UEM), PR (Brazil). Dept. de Analises Clinicas

    2009-07-01

    Phytochemical investigation from roots of Spathelia excelsa yielded the chromones 10(2,3-epoxy-3-methylbutanyl) spatheliachromen and 10(2,3-dihydroxy-3-methylbutanyl) methoxyspatheliacromen (5-methoxyspatheliabischromen); limonoid deacetylspathelin and protolimonoid C-21-epimers 3{beta}-angeloyloxy-7a,24,25-trihydroxy-21,23-oxide-14,18-cycloapotirucall -21-hemiacetal; the alkaloids 7,8-dimethoxyflindersin, casimiroin and N-methyl-4,7,8-trimethoxyquinolin-2(1H)-one, besides a mixture of {beta}-sitosterol and stigmasterol. Assays on promastigote forms of Leishmania braziliensis, deacetylspathelin showed moderate activity; and on epimastigote forms of Trypanossoma cruzi, 10(2,3-epoxy-3-methylbutanyl)spatheliachromen exhibited strong activity (IC50 = 11 {mu}g mL-1). (author)

  4. Study of root and leaf rachis of Spathelia excelsa: phytochemistry and activity against fungus Moniliophthora perniciosa associated with cupuassu (Theobroma grandiflorum); Estudo em raiz e raquis foliar de Spathelia excelsa: fitoquimica e atividade frente ao fungo Moniliophthora perniciosa associado ao cupuacuzeiro (Theobroma grandiflorum)

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Loretta Ennes de; Lima, Maria da Paz; Maximo, Ariane da Costa; Pereira, Elaine Cristina da Silva; Moreira, Wagner Alan dos Santos [Instituto Nacional de Pesquisas da Amazonia (INPA), Manaus, AM (Brazil). Coordenacao de Pesquisas em Tecnologia e Inovacao; Ferreira, Antonio Gilberto [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica; Veras, Solange de Mello [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil). Fac. de Ciencias Agrarias; Souza, Maria Geralda de, E-mail: mdapaz@inpa.gov.br [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Manaus, AM (Brazil)

    2012-07-01

    The chemical composition of Spathelia excelsa (Krause) R. S. Cowan and Brizicky was investigated and the limonoids harrisonin (1) and deacetylspathelin (2), alkaloids folinin and casimiroin mixture (3a,b), plus a further casimiroin (3b) were identified in methanol extract from root. The CH{sub 2}Cl{sub 2} extract from the rachis yielded protolimonoid 3{beta}-angeloyl-21,24-epoxy-7{alpha},21{alpha},23{alpha},25-tetrahydroxy-4{alpha},4{beta},8{beta},10{beta}-tetramethyl -25-dimethyl-14,18-cyclo-5{alpha},13{alpha},14{alpha},17{alpha}-cholestane (4), and methanol extract, the limonoids limonin diosphenol (5) and perforatin (6), as well as the chromone biflorin (7). Harrisonin and biflorin were isolated for the first time in this genus. On the antifungal assay against witches' broom (Moniliophthora perniciosa) compound 3b was found to be active. (author)

  5. Study of root and leaf rachis of Spathelia excelsa: phytochemistry and activity against fungus Moniliophthora perniciosa associated with cupuassu (Theobroma grandiflorum); Estudo em raiz e raquis foliar de Spathelia excelsa: fitoquimica e atividade frente ao fungo Moniliophthora perniciosa associado ao cupuacuzeiro (Theobroma grandiflorum)

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Loretta Ennes de; Lima, Maria da Paz; Maximo, Ariane da Costa; Pereira, Elaine Cristina da Silva; Moreira, Wagner Alan dos Santos [Instituto Nacional de Pesquisas da Amazonia (INPA), Manaus, AM (Brazil). Coordenacao de Pesquisas em Tecnologia e Inovacao; Ferreira, Antonio Gilberto [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica; Veras, Solange de Mello [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil). Fac. de Ciencias Agrarias; Souza, Maria Geralda de, E-mail: mdapaz@inpa.gov.br [Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), Manaus, AM (Brazil)

    2012-07-01

    The chemical composition of Spathelia excelsa (Krause) R. S. Cowan and Brizicky was investigated and the limonoids harrisonin (1) and deacetylspathelin (2), alkaloids folinin and casimiroin mixture (3a,b), plus a further casimiroin (3b) were identified in methanol extract from root. The CH{sub 2}Cl{sub 2} extract from the rachis yielded protolimonoid 3{beta}-angeloyl-21,24-epoxy-7{alpha},21{alpha},23{alpha},25-tetrahydroxy-4{alpha},4{beta},8{beta},10{beta}-tetramethyl -25-dimethyl-14,18-cyclo-5{alpha},13{alpha},14{alpha},17{alpha}-cholestane (4), and methanol extract, the limonoids limonin diosphenol (5) and perforatin (6), as well as the chromone biflorin (7). Harrisonin and biflorin were isolated for the first time in this genus. On the antifungal assay against witches' broom (Moniliophthora perniciosa) compound 3b was found to be active. (author)

  6. Phylogeny, evolutionary trends and classification of the Spathelia-Ptaeroxylon clade: morphological and molecular insights.

    Science.gov (United States)

    Appelhans, M S; Smets, E; Razafimandimbison, S G; Haevermans, T; van Marle, E J; Couloux, A; Rabarison, H; Randrianarivelojosia, M; Kessler, P J A

    2011-06-01

    The Spathelia-Ptaeroxylon clade is a group of morphologically diverse plants that have been classified together as a result of molecular phylogenetic studies. The clade is currently included in Rutaceae and recognized at a subfamilial level (Spathelioideae) despite the fact that most of its genera have traditionally been associated with other families and that there are no obvious morphological synapomorphies for the clade. The aim of the present study is to construct phylogenetic trees for the Spathelia-Ptaeroxylon clade and to investigate anatomical characters in order to decide whether it should be kept in Rutaceae or recognized at the familial level. Anatomical characters were plotted on a cladogram to help explain character evolution within the group. Moreover, phylogenetic relationships and generic limits within the clade are also addressed. A species-level phylogenetic analysis of the Spathelia-Ptaeroxylon clade based on five plastid DNA regions (rbcL, atpB, trnL-trnF, rps16 and psbA-trnH) was conducted using Bayesian, maximum parsimony and maximum likelihood methods. Leaf and seed anatomical characters of all genera were (re)investigated by light and scanning electron microscopy. With the exception of Spathelia, all genera of the Spathelila-Ptaeroxylon clade are monophyletic. The typical leaf and seed anatomical characters of Rutaceae were found. Further, the presence of oil cells in the leaves provides a possible synapomorphy for the clade. The Spathelia-Ptaeroxylon clade is well placed in Rutaceae and it is reasonable to unite the genera into one subfamily (Spathelioideae). We propose a new tribal classification of Spathelioideae. A narrow circumscription of Spathelia is established to make the genus monophyletic, and Sohnreyia is resurrected to accommodate the South American species of Spathelia. The most recent common ancestor of Spathelioideae probably had leaves with secretory cavities and oil cells, haplostemonous flowers with appendaged staminal

  7. Radioecology of Picea excelsa (L.) Lam

    International Nuclear Information System (INIS)

    Sarosiek, J.; Iwen, I.

    1976-01-01

    Within the area of the radiation anomaly near Kowary (Sudeten Mountains) the spruce (Picea excelsa) occurs in habitats with natural soil gamma radiation within 0.05-1.48 mR/h. By way of detailed ecological analysis 5 stentopic habitats of spruce development were selected differing in radiation intensity. In these habitats the intrapopulation variability, radioactivity and ecological properties of the Picea excelsa populations were investigated. It was demonstrated that radiation within the above mentioned range is an essential ecological limiting factor, conditioning ecotypic differences in Picea excelsa. The limiting influence of radiation is manifested in a high frequency of development anomalies in the spruce population. (author)

  8. Radioecology of Picea excelsa (L. Lam

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    Jan Sarosiek

    2015-01-01

    Full Text Available Within the area of the radiation anomaly near Kowary (Sudeten Mountains the spruce (Picea excelsa occurs in habitats with natural soil gamma radiation within 0.05-1.48 mR/h. By way of detailed ecological analysis 5 stenotopic habitats of spruce development were selected differing in radiation intensity. In these habitats the intrapopulation variability, radioactivity and ecological properties of the Picea excelsa populations were investigated. It was demonstrated that radiation within the above mentioned range is an essential ecological limiting factor, conditioning ecotypic differences in Picea excelsa. The limiting influence of radiation is manifested in a high frequency of development anomalies in the spruce population.

  9. Isolation of volatile compounds of Aloe excelsa (Berger)

    African Journals Online (AJOL)

    hope&shola

    2010-10-25

    Oct 25, 2010 ... carried out on Aloe ferox, Aloe vera as well as A. excelsa. (Speranza et al., 1986 ... perfumes, soaps, foods, chewing gums and beverages. (Venkatsen et al. ... excelsa gel is assumed to enhance the skin defense mechanisms.

  10. Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

    African Journals Online (AJOL)

    AJB SERVER

    identification of Aloin A and Aloe emodium has already been achieved in A. excelsa (Coopoosamy and Magwa,. 2006) Chrysophanol is known to occur in both commercial viable species of aloes (Aloe vera and A. ferox), but has not previously been isolated from A. excelsa. MATERIALS AND METHODS. Measurements.

  11. Control of Phytolyma lata Walker (Scott.) Attack on Milicia excelsa ...

    African Journals Online (AJOL)

    species plot (protected by mesh netting: T1), mixed-species plot (Milicia + Terminalia), mixed-species plot with foliar chemical treatment (T3), mono-species with foliar chemical treatment (T4) and untreated mono-species plot of Milicia excelsa ...

  12. Evaluation of herbal ointment formulation of Milicia excelsa (Welw ...

    African Journals Online (AJOL)

    Ijeoma

    2013-05-22

    May 22, 2013 ... 1Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Nsukka. 2Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka. Accepted 24 September, 2012. The wound healing effect of Milicia excelsa ointment was evaluated in ...

  13. Antibacterial activity of chrysophanol isolated from Aloe excelsa ...

    African Journals Online (AJOL)

    Extraction of the yellow colour compounds of leaves of Aloe excelsa were performed and 1,8-dihydroxy-3-methylanthracenedione (chrysophanol) was isolated and tested for antibacterial activities against four gram negative and five gram positive bacterial strains. The structures of chrysophanol was determined by chemical ...

  14. Comparative analysis of essential oil contents of Juniperus excelsa ...

    African Journals Online (AJOL)

    Cones/berries of Juniperus excelsa from three provenances in Balochistan, Pakistan were collected and essential oil was extracted by solvent method. Oil contents were analyzed on gas chromatography mass spectrometry (GCMS). Identification and quantification was made by using Wiley and NIST spectral library and HP ...

  15. Anticancer Activity of Limonia acidissima Linn (Rutaceae) Fruit ...

    African Journals Online (AJOL)

    Rutaceae) which has long been used traditionally for various infectious and malignant diseases. Methods: The fruit extract of Limonia acidissima Linn was obtained by macerating 3 times with methanol and then concentrating it at reduced pressure.

  16. Azadirachtin derivatives from seed kernels of Azadirachta excelsa.

    Science.gov (United States)

    Kanokmedhakul, Somdej; Kanokmedhakul, Kwanjai; Prajuabsuk, Thirada; Panichajakul, Sanha; Panyamee, Piyanan; Prabpai, Samran; Kongsaeree, Palangpon

    2005-07-01

    Three new azadirachtin derivatives, named azadirachtins O-Q (1-3), along with the known azadirachtin B (4), azadirachtin L (5), azadirachtin M (6) 11alpha-azadirachtin H (7), 11beta-azadirachtin H (8), and azadirachtol (9) were isolated from seed kernels of Azadirachta excelsa. Their structures were established by spectroscopic techniques, and the structure of 3 was confirmed by X-ray analysis. Compounds 1-7 and 9 exhibited toxicity to the diamondback moth (Plutella xylostella) with an LD50 of 0.75-1.92 microg/g body weight, in 92 h.

  17. Disposition of two names in Almeidea (Rutaceae Disposição de dois nomes em Almeidea (Rutaceae

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    Milton Groppo

    2010-12-01

    Full Text Available Examination of type specimens at the P herbarium showed that Almeidea longifolia A. St.-Hil. (Rutaceae is an illegitimate substitute name for A. affinis A. St.-Hil. The latter name is proposed here as a heterotypic synonym of A. rubra A. St.-Hil.O estudo dos tipos nomenclatórios no herbário P mostrou que Almeidea longifolia A. St.-Hil. (Rutaceae é um nome substituto ilegítimo de Almeidea affinis A. St.-Hil., e este último nome é aqui considerado sinônimo heterotípico de A. rubra A. St.-Hil.

  18. Coumarin and alkaloids of Rauia resinosa (Rutaceae); Cumarinas e alcaloides de Rauia resinosa (Rutaceae)

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    Albarici, Tatiane Regina; Vieira, Paulo Cezar; Fernandes, Joao Batista; Silva, Maria Fatima das Gracas Fernandes da, E-mail: paulo@dq.ufscar.b [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica; Pirani, Jose Rubens [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Biociencias. Dept. de Botanica

    2010-07-01

    The genus Rauia, that is poorly chemically studied, belongs to the Rutaceae family. This family has been known to contain a large variety of secondary metabolites. Our phytochemical investigation of the stem and leaves of Rauia resinosa has led to the identification of the structurally related coumarins: murralongin (1), murrangatin (2), munomicrolin (3), murrangatin diacetate (4), umbelliferone (5), rauianin (6) and one novel coumarin: 3-ethylrauianin (7); the alkaloids: N-methyl-4-methoxy-2-quinolone (8), mirtopsine (9), dictamine (10), g-fagarine (11), skimmianine (12), Z-dimethylrhoifolinate (13), zantodioline (14), zantobungeanine (15), veprissine (16), one novel alkaloid 7-hydroxy-8-methoxy-N-methylflindersine (17) and 8-hydroxy-N-methylflindersine (18) that is described as a natural product for the first time, and a mixture of steroids: as sitosterol and stigmasterol. (author)

  19. Antiasthmatic and antiallergic potential of methanolic extract of leaves of Ailanthus excelsa

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    Dinesh Kumar

    2011-02-01

    Full Text Available The aim of study was antiasthmatic potential of methanolic extract of leaves of Ailanthus excelsa Roxb., Simaroubaceae. Traditionally or in Indian system of medicine, A. excelsa is used in the treatment of asthma, cough, colic pain, cancer, diabetes and also used as antispasmodic, antifertility, bronchodilator. Stem bark of A. excelsa already reported for its potential against asthma. The pollens of Ailanthus excelsa reported allergic in nature and the time of collection of leaves were important in this study, generally the flowering stage of plant was avoided for the collection due to maximum chance of pollens at that time. Methanolic extract of leaves of A. excelsa was evaluated using in vitro goat tracheal chain preparation model and in vivo- Milk induced leucocytosis, eosinophilia, Clonidine induced catalepsy in mice model while Passive paw anaphylaxis and Clonidine induced mast cell degranulation in rat model. The extract showed the presence of flavonoids, terpenoids, saponins, quassonoids and test was also positive for alkaloids and steroids. The extract also showed the presence of quercetin which is flavonoid and detected on the preparative TLC plate with the help of standard quercetin. Dose response studies of methanolic extract of leaves of A. excelsa Roxb. were conducted at 100 µg mL-1 in vitro and 100, 200, 400 mg kg-1 p.o. in vivo models. The treatment with methanolic extract of A. excelsa at different dose level showed the significant (*p<0.05, **p<0.01, ***p<0.001 antiasthmatic activity. Inhibition or decrease the release of inflammatory mediators potentiates the antiasthmatic as well as antiallergic activity of methanolic extract of leaves of A. excelsa.

  20. Prospect of Milicia excelsa (Welw. C. Berg for Multi-Tree Species Agroforestry

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    Alfred Ossai Onefeli

    2015-11-01

    Full Text Available Background and Purpose: The population of most of our economically indigenous tree species in Nigeria is declining. Human activities and agricultural practices have been the ultimate contributors to this decrease. In order to ameliorate the conflict between agriculture and forestry, agroforestry was introduced. However, most of the practiced agroforestry is based on single tree species. Agroforestry practiced using single tree species have been reported to be ecologically staggered and therefore it is pertinent that phytosociology of trees with agroforestry potential is studied in order to improve the sustainability of human livelihood. Materials and Methods: This study was carried out in the University of Ibadan’s campus forest. The data were collected on Milicia excelsa (Welw. C. Berg by enumerating the tree species and also by identifying and enumerating the tree species associated with the subject tree (Milicia excelsa. Statistical analysis was done using percentages, Chi-square and charts. Results: A total of 49 individual Milicia excelsa were encountered in the study area. The results show 31 woody tree species associated with Milicia excelsa. Of all the associates Azadirachta indica A.Juss. happened to be the best one, having an average distance of 5.4 m to the subject tree. The sex ratio of Milicia excelsa was discovered to be approximately 1:1. Conclusions: Based on the obtained results of this research it may be concluded that Milicia excelsa has the prospect of being used in agroforestry in multi-tree species systems.

  1. Furochinoline alkaloids in plants from Rutaceae family – a review

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    Adamska-Szewczyk Aldona

    2016-04-01

    Full Text Available Over the past five years, phytochemical and pharmacological studies have been conducted on material extracted from members of the Rutaceae family. In such work, new furochinoline-structured alkaloids were isolated from Ruta sp. and Dictamnus sp. Beyond the aforementioned, other substances with promising activity were isolated from the less-known species of Zanthoxylum, Evodia, Lonchocarpus, Myrthopsis and Teclea. Currently used forms of extraction, as well as methods of isolation and detection, allow the obtaining of pure, biologically active compounds. Many of these have antifungal, anti-bacterial and anti-plasmodial properties. Others are still being researched as potential drugs, which, in future, may be used in treating those afflicted with HIV and cancer. This article is designed to give the readers a thorough review of the active natural products from the Rutaceae family.

  2. Rutaceae endémicas del Perú

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    Blanca León

    2013-10-01

    Full Text Available La familia Rutaceae es reconocida en el Perú por presentar 25 géneros y 76 especies (Brako & Zarucchi, 1993; Ulloa Ulloa et al., 2004, principalmente árboles y arbustos. En este trabajo reconocemos seis endemismos, representando igual número de géneros. Los taxones endémicos se encuentran en las regiones Bosques Húmedos Amazónicos, Bosques Muy Húmedos Premontanos y Mesoandina, entre los 130 y 2000 m de altitud. Tres taxones se encuentran representados dentro del Sistema Nacional de Áreas Naturales Protegidas por el Estado.

  3. Melicimides A and B: Two New Ceramides from Stem Bark of Melicia excelsa

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    Hidayat Hussain

    2013-03-01

    Full Text Available Melicimides A (1 and B (2, two new ceremides have been isolated from Melicia excelsa ( Moraceae. The structures of the new compounds were determined by comprehensive analyses of their 1D ( 1H and 13C NMR, 2D NMR (including COSY, HMQC, and HMBC and ESIMS spectral data. A known compound viz., β-sitosterol has also been identified.

  4. Chemical constituents of leaves of Senna spectabilis (DC) Irwin and Barneby var. excelsa (Schard.) Irwin and Barneby; Constituintes quimicos das folhas de Senna spectabilis (DC) Irwin and Barneby var. excelsa (Schrad.) Irwin and Barneby

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    Silva, Fabio de Oliveira; Oliveira, Irvila Ricarte de; Silva, Maria Goretti de Vasconcelos, E-mail: mgvsilva@ufc.b [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica; Braz Filho, Raimundo [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacazes, RJ (Brazil)

    2010-07-01

    From leaves of Senna spectabilis var. excelsa were isolated caffeine, the triterpenes lupeol, {alpha}-amyrin, {beta}-amyrin, cycloeucalenol, friedelin and ursolic, oleanolic and betulinic acids, besides the steroids sitosterol and stigmasterol and their respective glucosides. The structures of these compounds were established by spectroscopic analysis including two-dimensional NMR methods and comparison with published spectral data. This paper deals with the first report of these compounds in S. spectabilis var. excelsa. (author)

  5. ANATOMÍA FOLIAR DE PILOCARPUS GOUDOTIANUS TUL. (RUTACEAE

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    HERMOSO GALLARDO LUIS

    2002-12-01

    Full Text Available El género Pilocarpus (Rutaceae, distribuido en la región neotropical, constituye unrecurso de gran importancia en el campo de la medicina, como materia prima para laobtención del nitrato de pilocarpina, el cual se utiliza en el tratamiento del glaucoma,y se obtiene de las hojas de estas plantas. Este estudio permitió la caracterizaciónanatómica de la hoja de Pilocarpus goudotianus Tul., encontrándose rasgosxeromorfos, tales como cutícula gruesa, paredes epidérmicas externas cutinizadas,alta densidad estomática y casquetes de esclerénquima rodeando los hacesvasculares. Estos rasgos podrían considerarse como adaptaciones anatómicas alambiente seco donde esta especie se desarrolla.

  6. Site properties for Crimean juniper (Juniperus excelsa) in semi-natural forests of south western Anatolia, Turkey.

    Science.gov (United States)

    Ozkan, Kürsad; Gulsoy, Serkan; Aerts, Raf; Muys, Bart

    2010-01-01

    We explored the semi-natural forests in south western Anatolia along a gradient between Mediterranean and continental climates to determine the site requirements of Juniperus excelsa in Turkey. We hypothesized that environmental variables and indicator species can be used to predict differences in occurrence and cover of J. excelsa and can therefore support decision making in reforestation management planning. Plant species composition and environmental variables were assessed in 153 plots. Association between J. excelsa and other plant species and environmental variables were analyzed using Fisher exact probability tests and stepwise discriminant analysis. High altitude (> 1000 m) as a proxy for an Oromediterranean climate, and high surface stoniness as a proxy for low competition by other tree species, are positive site properties for J. excelsa. The tree species avoids Eumediterranean and Supramediterranean plant communities. Twelve plant species, including the herbs Dianthus zonatus, Ajuga chamaepitys and Paronchia carica and the shrub Cotoneaster nummularia may be used as site indicators for J. excelsa restoration. Platanus orientalis, with similar site requirements but at present negatively associated to J. excelsa due to competitive effects, may be considered an additional indicator if stand conversion (harvesting and replacing P. orientalis) is part of the management plan.

  7. Micropropagation of Araucaria excelsa R. Br. var. glauca Carrière from orthotropic stem explants.

    Science.gov (United States)

    Sarmast, Mostafa Khoshhal; Salehi, Hassan; Khosh-Khui, Morteza

    2012-07-01

    The objectives of the present work were in vitro propagation of Araucaria excelsa R. Br. var. glauca Carrière (Norfolk Island pine) with focus on the evaluation of the mean number of shoots per explant (MNS/E) and mean length of shoots per explants (MLS/E) produced by different parts of the orthotropic stem of A. excelsa R. Br. var. glauca in response to plant growth regulators. Norfolk Island pine axillary meristems responded very well to the 2-iso-pentenyl adenine (2iP) and thidiazuron (TDZ) levels. Explants taken from stem upper segments in the media containing 2iP had a higher MNS/E (3.47) and MLS/E (6.27 mm) in comparison to those taken from stem lower segments, which were 0.71 and 0.51 mm, respectively. Using 0.045 μM TDZ in the MS medium not only resulted in 4.60 MNS/E with 7.08 mm MLS/E but proliferated shoots showed a good performance as well. Investigating the best position of stem explant on mother plant as well as the best concentrations of growth regulators were performed which were useful for efficient micropropagation of this plant. Thirty three percent of explants were rooted in the MS medium containing 3 % sucrose, supplemented with 7.5 μM of both NAA and IBA for 2 weeks before transferring to a half strength MS medium without any growth regulator. Plantlets obtained were acclimatized and transferred to the greenhouse with less than 20 % mortality. This procedure considered the first successful report for regeneration and acclimatization of A. excelsa R. Br. var. glauca plantlet through main stem explants.

  8. [Genetical control of the allozymes in juniper (Juniperus excelsa Bieb.) of the Crimea].

    Science.gov (United States)

    Korshikov, I I; Nikolaeva, A V

    2007-01-01

    Genetical control of nine enzyme systems has been studied in preserved juniper species (Juniperus excelsa Bieb.) of the natural population of the mountain Crimea. Isozymes were extracted from the haploid seed endosperms and separated elecrophoretically. As a result 16 loci have been identified. Fourteen of them were polymorphic (14--Gdh, Got-1, Mdh-1, Mdh-2, Mdh-3, Acp-1, Acp-2, Acp-3, Lap-1, Dia-1, Fdh, Sod-1, Sod-2, Sod-3). Analysis of the allele segragation of the heterozygous trees confirmed their monogenic inheritance.

  9. Uncovering spatial patterns in the natural and human history of Brazil nut (Bertholletia excelsa) across the Amazon Basin

    NARCIS (Netherlands)

    Thomas, E.; Alcázar Caicedo, C.; McMichael, C.H.; Corvera, R.; Loo, J.

    2015-01-01

    Aim Our goal was to test the hypothesis that ancient humans substantially contributed to shaping the current distribution of Brazil nut (Bertholletia excelsa), an Amazonian tree species that has been important for human livelihoods since pre-Columbian times. We scrutinized the putative association

  10. Isoflavonoids in the Rutaceae family: 1. Fortunella obovata, Murraya paniculata and four Citrus species.

    Science.gov (United States)

    Lapcík, Oldrich; Klejdus, Borivoj; Davidová, Michaela; Kokoska, Ladislav; Kubán, Vlastimil; Moravcová, Jitka

    2004-01-01

    Several types of compounds with immunoreactivity similar to isoflavonoids were detected in water: ethanol extracts of leaves of Fortunella obovata Hort. ex Tanaka, Murraya paniculata Jack. and four Citrus species, namely C. aurantium L, C. grandis Osbeck, C. limonia Osbeck., and C. sinensis Osbeck (Rutaceae). The chromatographic mobilities of the immunoreactive substances were compared with those of authentic standards, revealing a spectrum of isoflavonoid metabolites in all plants studied. Aglycones as well as glycosides were recognized, namely daidzin, genistin, daidzein, genistein, formononetin, biochanin A, prunetin, and several incompletely characterized isoflavonoids. A subsequent HPLC-MS study verified the identities of the main immunoreactive isoflavonoids and established the identities of several others, viz. glycitein, glycitin, ononin and sissotrin, including the malonylated and acetylated isoflavonoid glucosides. The estimated content of the individual immunoreactive entities ranged from a few microg to about 2 mg/kg (dry weight). It is concluded that the isoflavonoid metabolic pathway is present throughout the Rutaceae family.

  11. A new species of Raputia (Rutaceae) from the Selva Central of Peru.

    Science.gov (United States)

    Fernandez-Hilario, Robin; Arteaga, Robert

    2017-01-01

    Raputia codo-pozuzoensis Rob. Fernandez & Arteaga, sp. nov. (Galipeinae, Rutaceae), a new species endemic to the Huanuco region, in the Selva Central of Peru, is described and illustrated here. The new species differs from other species of Raputia by the combination of 5-7-foliolate leaves (a new characteristic for the genus) and longer petioles. A key to the four Peruvian species of Raputia is presented.

  12. A new species of Raputia (Rutaceae from the Selva Central of Peru

    Directory of Open Access Journals (Sweden)

    Robin Fernandez-Hilario

    2017-11-01

    Full Text Available Raputia codo-pozuzoensis Rob. Fernandez & Arteaga, sp. nov. (Galipeinae, Rutaceae, a new species endemic to the Huanuco region, in the Selva Central of Peru, is described and illustrated here. The new species differs from other species of Raputia by the combination of 5–7-foliolate leaves (a new characteristic for the genus and longer petioles. A key to the four Peruvian species of Raputia is presented.

  13. The Review of Certain In Vivo Antioxidant Effects on Essential Oils of Origanum Minutiflorum O Schwarz-Ph Davis, Juniperus Excelsa Bieb.subsp. Excelsa and Histopathologic Changes

    Directory of Open Access Journals (Sweden)

    I Göze

    Full Text Available ABSTRACT Essential oil of plants called Juniperus excelsa Bieb. (JE, Origanum minutiflorum O. Schwarz and P.H. Davis (OM were used in this study. In order to determine experimental doses, LD50 values of essential oils were determined on mice. Taking into consideration the LD30 range, the experimental toxic doses were calculated for each rat (rat/kg. The toxic dosages thus determined were adapted to rats for active substances (rat/kg. Using commercially available pure virgin olive oil (VOO as the solvent and diluting agent, OM oil (n=10, JE fruitoil (n=10, carvacrol (CRV (n=10, VOO (n=10 and normal saline SF (n=8 were administered on the basis of 12 days into intraperitoneal (IP. Enzyme activities of Glucose-6-Phosphate dehydrogenase (G6PDH, malate dehydrogenase (MDH, Superoxide Dismutase (SOD, Glutathione-S-transferase (GST, Adenosine-deaminase (ADA and Catalase were studied in isolates of kidney, brain and liver tissues. The data was statistically analyzed through Kruskal Wallis variance analysis. Elevated levels of GST and catalase have been found statistically important, as have both essential oil activities of OM and JE in the kidney tissue (p<0.005. All of the enzymes except the levels of ADA and SOD led to a statistically significant change in the brain and liver. There was sinusoidal hyperemia and capsular adhesion in the liver as histopathological were found to be statistically significant (p<0.005. It did not observe any important changes in the other organs. Findings were scored and analyzed by using x2(chi-square test and Fisher’s definite variance analysis.

  14. Antioxidant activity and chemical composition of Juniperus excelsa ssp. polycarpos wood extracts.

    Science.gov (United States)

    Hosseinihashemi, S K; Dadpour, A; Lashgari, A

    2017-03-01

    Extracts from the wood of Juniperus excelsa ssp. polycarpos were analysed for their antioxidant activity using the DPPH method and compared with ascorbic acid and butylated hydroxytoluene. The most active extracts were analysed for their chemical composition using gas chromatography-mass spectrometry. Acetone extract was found to be moderately active as an antioxidant agent at 58.38%, which was lower than the value of vitamin C (98.56%) at the concentration of 14.20 mg/mL. The major components identified in the acetone extract as trimethylsilyl (TMS) derivatives were pimaric acid TMS (24.56%), followed by α-d-glucopyranoside,1,3,4,6-tetrakis-O-(TMS)-β-d-fructofuranosyl 2,3,4,6-tetrakis-O-(TMS) (21.39%), triflouromethyl-bis-(TMS)methyl ketone (9.32%), and cedrol (0.72%). The dissolved water:methanol (1:1 v/v) partitioned from acetone extract afforded 12 fractions; among them, the F9 fraction was found to have good antioxidant activity (88.49%) at the concentration of 14.20 mg/mL. The major compounds identified in F9 fraction were α-d-glucopyranoside, 1,3,4,6-tetrakis-O-(TMS) (20.22%) and trifluoromethyl-bis-(TMS)methyl ketone (5.10%).

  15. BIOMASSA, CRESCIMENTO E RESPOSTAS ECOFISIOLÓGICAS DE PLANTAS JOVENS DE Bertholletia excelsa BONPL. SUBMETIDAS A DIFERENTES NÍVEIS DE IRRADIÂNCIA

    Directory of Open Access Journals (Sweden)

    Cristiane Santos do Carmo Ribeiro de Souza

    2017-01-01

    Full Text Available Brazil nut ( Bertholletia excelsa Bonpl. is a light-demanding species, because in natural forest, the species depending of formation of clearings for reach reproduction size and in forest plantation, showing fast initial growth when exposed to high levels of irradiance. However, the ecophysiological traits of this species under contrasting conditions of irradiance were not investigated. In this study, in addition to growth traits, we investigated for first time the degree of plasticity of ecophysiological response of Bertholletia excelsa when subjected to contrasting irradiance environments. Young plants of Bertholletia excelsa were cultivated under three conditions of irradiance: low (20-300 μmol m -2 s -1 , moderate (800-1000 μmol m -2 s -1 and high (1900-2100 μmol m -2 s -1 . We analyzed the growth traits, gas exchange, chloroplastid pigment contents and, the end of experiment, the accumulation and partitioning of biomass. Young plants of Bertholletia excelsa showed the highest values of biomass, growth and photosynthesis when exposed to environments of moderate and high irradiance. Low irradiance condition stimulated more biomass partitioning for shoot and chloroplastid pigment contents. Bertholletia excelsa showed physiological plasticity under contrasting conditions of irradiance, the largest growth and biomass accumulation in environments of moderate and high irradiance were promoted by better photosynthetic performance, whereas the positive carbon balance under low irradiance is ensured by investment in structures of interception and energy harvesting.

  16. Morphological Variation and Ecological Structure of Iroko (Milicia excelsa Welw. C.C. Berg) Populations across Different Bio geographical Zones in Benin

    International Nuclear Information System (INIS)

    Ouinsavi, C.; Sokpon, N.

    2010-01-01

    Iroko (Milicia excelsa) is a commercially important timber tree species formerly known by local people in Benin. Because of the highly attractive technological properties of its wood and its multipurpose uses, the species was subjected to intensive human pressure. Apart from strong climate oscillation during the Pleistocene, human caused habitat fragmentation through continuous land clearing for agriculture, extensive forests exploitation and urbanization induced the occurrence of many isolated forest plots and trees species among which Milicia excelsa trees. As fragmentation was proved to have deleterious effects on genetic diversity within a species and its morphological structure, it was of interest to investigate the current demographic, morphological and genetic structure of M. excelsa before coming up with conservation strategies. In the current study, morphological variation and ecological structure of M. excelsa populations were assessed in Benin using transect sampling method and multivariate analyses including principal component, cluster and canonical discriminant analyses. On the basis of morphological parameters, M. excelsa individuals and populations were clustered into four and discrimination of groups indicated that most of variations were highly related to edaphic factors and annual rainfall. Erratic diameter distribution was found for many populations although most of them showed bell shaped diameter distribution.

  17. Phytochemical screening and antimicrobial activity of roots of Murraya koenigii (Linn. Spreng. (Rutaceae

    Directory of Open Access Journals (Sweden)

    Manisha Vats

    2011-12-01

    Full Text Available Murraya koenigii, family Rutaceae, commonly known as Curry leaf plant is a highly valued plant for its medicinal value and characteristic aroma. The plant is a rich source of carbazole alkaloids. The petroleum ether, chloroform, ethyl acetate and ethanol extracts of roots of the plant were screened for phytochemical properties and antimicrobial activity for Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. Phytochemical screening showed the presence of carbohydrates, alkaloids, steroids and flavonoids in the root extracts of the plant. The study shows that all the extracts possess remarkable antibacterial activity. Additionally, petroleum ether and chloroform extracts also had antifungal activity.

  18. Anointing chemicals and ectoparasites: responses by ticks and mosquitoes to Citrus (Rutaceae) peel exudates and monoterpene constituents

    Science.gov (United States)

    Some birds and mammals rub their feathers or fur with the fruits or leaves of Citrus spp. or other Rutaceae, presumably to deter ectoparasites. We measured avoidance and other responses by the lone star tick (Amblyomma americanum) and the yellow fever mosquito (Aedes aegypti) to lemon peel exudate a...

  19. Genetic Diversity and Population Structure of a Threatened African Tree Species, Milicia excelsa, Using Nuclear Micro satellites DNA Markers

    International Nuclear Information System (INIS)

    Ouinsavi, Ch.; Sokpon, N.; Ouinsavi, Ch.; Khasa, D.P.

    2009-01-01

    To accurately estimate the genetic diversity and population structure for improved conservation planning of Milicia excelsa tree, 212 individuals from twelve population samples covering the species' range in Benin were surveyed at seven specific micro satellite DNA loci. All loci were variable, with the mean number of alleles per locus ranging from 5.86 to 7.69. Considerable genetic variability was detected for all populations at the seven loci (AR=4.60; HE=0.811). Moderate but statistically significant genetic differentiation was found among populations considering both FST (0.112) and RST (0.342). All of the populations showed heterozygosity deficits in test of Hardy-Weinberg Equilibrium and significantly positive FIS values due to inbreeding occurring in the species. Pairwise FST values were positively and significantly correlated with geographical distances (r=0.432; P=.007, Mantel's test) indicating that populations are differentiated by isolation by distance. Bayesian analysis of population structure showed division of the genetic variation into four clusters revealing the existence of heterogeneity in population genetic structure. Altogether, these results indicate that genetic variation in Milicia excelsa is geographically structured. Information gained from this study also emphasized the need for in situ conservation of the relict populations and establishment of gene flow corridors through agroforestry systems for interconnecting these remnant populations.

  20. Comparative Studies on the Phytochemistry of Essential Oil from Needles and Berries of Juniperus excelsa M. Bieb

    International Nuclear Information System (INIS)

    Nadir, M.

    2013-01-01

    The study provides a comparison on the essential oil composition of Juniperus excelsa M. Bieb. Essential oil extracted separately from berries (yield 0.11%) and needles (yield 0.13%) were subjected to GC-MS and GC-FID techniques for the identification and quantification of the constituents. A total of 26 compounds; 21 from berries and 20 from needles were identified. Of these major constituents in berries were beta-pinene (43.4%), beta-pinene (32.3%), limonene (9.6%), and sabinene (2.7%) while in needles were beta-pinene (36.0%), beta-pinene (30.2%), limonene (12.6%), and beta-phellandrene (3.9%). 3 identified compounds (guaiol, beta-elemene and epi-bicyclosesquiphellandrene) were not reported yet from any essential oil of J. excelsa, beta-guaiene is also reported for the first time from needles. A comparative discussion on the chemical composition of the essential oil from two different parts of plants in the current study with those available in literature is also included. The findings confirmed that the Pakistan origin plant belongs to alpha-pinene chemotype, alpha-pinene rich oils are used as local antiseptic. (author)

  1. Demography of the Brazil nut tree (Bertholletia excelsa) in the Bolivian Amazon : impact of seed extraction on recruitment and population dynamics

    NARCIS (Netherlands)

    Zuidema, P.A.; Boot, R.G.A.

    2002-01-01

    A demographic study was carried out on Bertholletia excelsa, the Brazil nut tree, in two primary forest sites in Northern Bolivia where Brazil nuts have been harvested for several decades. In spite of the large proportion (93€of seeds that are harvested, reasonable densities of recently emerged

  2. Nut Production in Bertholletia excelsa across a Logged Forest Mosaic: Implications for Multiple Forest Use

    Science.gov (United States)

    Rockwell, Cara A.; Guariguata, Manuel R.; Menton, Mary; Arroyo Quispe, Eriks; Quaedvlieg, Julia; Warren-Thomas, Eleanor; Fernandez Silva, Harol; Jurado Rojas, Edwin Eduardo; Kohagura Arrunátegui, José Andrés Hideki; Meza Vega, Luis Alberto; Revilla Vera, Olivia; Valera Tito, Jonatan Frank; Villarroel Panduro, Betxy Tabita; Yucra Salas, Juan José

    2015-01-01

    Although many examples of multiple-use forest management may be found in tropical smallholder systems, few studies provide empirical support for the integration of selective timber harvesting with non-timber forest product (NTFP) extraction. Brazil nut (Bertholletia excelsa, Lecythidaceae) is one of the world’s most economically-important NTFP species extracted almost entirely from natural forests across the Amazon Basin. An obligate out-crosser, Brazil nut flowers are pollinated by large-bodied bees, a process resulting in a hard round fruit that takes up to 14 months to mature. As many smallholders turn to the financial security provided by timber, Brazil nut fruits are increasingly being harvested in logged forests. We tested the influence of tree and stand-level covariates (distance to nearest cut stump and local logging intensity) on total nut production at the individual tree level in five recently logged Brazil nut concessions covering about 4000 ha of forest in Madre de Dios, Peru. Our field team accompanied Brazil nut harvesters during the traditional harvest period (January-April 2012 and January-April 2013) in order to collect data on fruit production. Three hundred and ninety-nine (approximately 80%) of the 499 trees included in this study were at least 100 m from the nearest cut stump, suggesting that concessionaires avoid logging near adult Brazil nut trees. Yet even for those trees on the edge of logging gaps, distance to nearest cut stump and local logging intensity did not have a statistically significant influence on Brazil nut production at the applied logging intensities (typically 1–2 timber trees removed per ha). In one concession where at least 4 trees ha-1 were removed, however, the logging intensity covariate resulted in a marginally significant (0.09) P value, highlighting a potential risk for a drop in nut production at higher intensities. While we do not suggest that logging activities should be completely avoided in Brazil nut rich

  3. Nut Production in Bertholletia excelsa across a Logged Forest Mosaic: Implications for Multiple Forest Use.

    Directory of Open Access Journals (Sweden)

    Cara A Rockwell

    Full Text Available Although many examples of multiple-use forest management may be found in tropical smallholder systems, few studies provide empirical support for the integration of selective timber harvesting with non-timber forest product (NTFP extraction. Brazil nut (Bertholletia excelsa, Lecythidaceae is one of the world's most economically-important NTFP species extracted almost entirely from natural forests across the Amazon Basin. An obligate out-crosser, Brazil nut flowers are pollinated by large-bodied bees, a process resulting in a hard round fruit that takes up to 14 months to mature. As many smallholders turn to the financial security provided by timber, Brazil nut fruits are increasingly being harvested in logged forests. We tested the influence of tree and stand-level covariates (distance to nearest cut stump and local logging intensity on total nut production at the individual tree level in five recently logged Brazil nut concessions covering about 4000 ha of forest in Madre de Dios, Peru. Our field team accompanied Brazil nut harvesters during the traditional harvest period (January-April 2012 and January-April 2013 in order to collect data on fruit production. Three hundred and ninety-nine (approximately 80% of the 499 trees included in this study were at least 100 m from the nearest cut stump, suggesting that concessionaires avoid logging near adult Brazil nut trees. Yet even for those trees on the edge of logging gaps, distance to nearest cut stump and local logging intensity did not have a statistically significant influence on Brazil nut production at the applied logging intensities (typically 1-2 timber trees removed per ha. In one concession where at least 4 trees ha-1 were removed, however, the logging intensity covariate resulted in a marginally significant (0.09 P value, highlighting a potential risk for a drop in nut production at higher intensities. While we do not suggest that logging activities should be completely avoided in Brazil

  4. A molecular phylogeny of the orange subfamily(Rutaceae: Aurantioideae) using nine cpDNA sequences.

    Science.gov (United States)

    Bayer, Randall J; Mabberley, David J; Morton, Cynthia; Miller, Cathy H; Sharma, Ish K; Pfeil, Bernard E; Rich, Sarah; Hitchcock, Roberta; Sykes, Steve

    2009-03-01

    The breeding of new, high-quality citrus cultivars depends on dependable information about the relationships of taxa within the tribe Citreae; therefore, it is important to have a well-supported phylogeny of the relationships between species not only to advance breeding strategies, but also to advance conservation strategies for the wild taxa. The recent history of the systematics of Citrus (Rutaceae: Aurantioideae) and its allies, in the context of Rutaceae taxonomy as a whole, is reviewed. The most recent classification is tested using nine cpDNA sequence regions in representatives of all genera of the subfam. Aurantioideae (save Limnocitrus) and numerous species and hybrids referred to Citrus s.l. Aurantioideae are confirmed as monophyletic. Within Aurantioideae, tribe Clauseneae are not monophyletic unless Murraya s.s. and Merrillia are removed to Aurantieae. Within tribe Aurantieae, the three traditionally recognized subtribes are not monophyletic. Triphasiinae is not monophyletic unless Oxanthera is returned to Citrus (Citrinae). Balsamocitrinae is polyphyletic. Feroniella, traditionally considered allied closely to Limonia (=Feronia), is shown to be nested in Citrus. The proposed congenericity of Severinia and Atalantia is confirmed. The most recent circumscription of Citrus is strongly supported by this analysis, with hybrids appearing with their putative maternal parents. The genus was resolved into two clades, one comprising wild species from New Guinea, Australia, and New Caledonia (formerly Clymenia, Eremocitrus, Microcitrus, Oxanthera), but surprisingly also Citrus medica, traditionally believed to be native in India. The second clade is largely from the Asian mainland (including species formerly referred to Fortunella and Poncirus).

  5. Identification of Endophytic Fungi of Medicinal Herbs of Lauraceae and Rutaceae with Antimicrobial Property

    Directory of Open Access Journals (Sweden)

    Min-Yuan Ho

    2012-09-01

    Full Text Available This study was conducted to determine taxonomical features and antimicrobial activities of 156 isolates of endophytic fungi collected from twigs of medicinal plants of Lauraceae (67 isolates and Rutaceae (89 isolates in central and northern Taiwan. The 156 isolates of fungi were classified into 35 genera in 19 families based on morphological characteristics of mycelia and asexual/sexual spores, as well as molecular phylogenetic analysis of rDNA LSU D1/D2 and ITS regions. The most common endophytes were in the taxa of Colletotrichum, Guignardia, Hypoxylon, Nigrospora, Phomopsis and Xylaria, and the most common hosts were Citrus and Zanthoxylum of Rutaceae and Cinnamomum of Lauraceae. Molecular phylogenetic analysis showed that xylariaceous isolates could be separated into Xylaria and Hypoxylon groups based on rDNA of LSU D1/D2 and ITS regions. Four isolates of endophytic fungi including Lasmenia sp. isolate CB10, Ophioceras tenuisporum isolate CI02, Xylaria cubensis isolate LA04 and Cyanodermella sp. isolate TR09 were tested for antimicrobial activities using a dual culture method and Lasmenia sp. isolate CB10 and Cyanodermella sp. isolate TR09 showed better antimicrobial activity against 12 plant pathogens including 9 fungi and 3 bacteria. Spraying Chinese cabbage (Brassica rapa plants with culture filtrates of the endophytic fungus Lasmenia sp. isolate CB10 significantly reduced severity of anthracnose of Chinese cabbage caused by Colletotrichum higginsianum under greenhouse conditions. This study suggests that the Lasmenia sp. isolate CB10 may be of potential for management of anthracnose of Chinese cabbage.

  6. Interactions between carpenter bees and orchid bees (Hymenoptera: Apidae in flowers of Bertholletia excelsa Bonpl. (Lecythidaceae Interações entre abelhas carpinteiras e abelhas das orquídeas (Hymenoptera: Apidae em flores de Bertholletia excelsa Bonpl. (Lecythidaceae

    Directory of Open Access Journals (Sweden)

    Charles Fernando dos Santos

    2012-03-01

    Full Text Available Competition between two species of bees for the same type of floral resource may generate antagonistic behavior between them, especially in cultivated areas where food resources are limited, seasonally and locally. In this study, was tested the hypothesis of antagonism between two solitary bee species of the family Apidae, Eulaema mocsaryi (Euglossini and Xylocopa frontalis (Xylocopini, visiting the Brazil nut flowers (Bertholletia excelsa: Lecythidaceae in a central Amazonia agricultural area. The visitation time was analyzed to detect the possible temporal overlap in the foraging of these bees. Furthermore, was analyzed their interspecific interactions for manipulating flower species visited by an opponent species, as well as attempts to attack this opponent. The individuals of Xylocopa frontalis visited the Brazil nut flowers before Eulaema mocsaryi, although the peak visitation of both did not presented significant differences. Neither of the species manipulated flowers recently visited by opponent species, and there were practically no antagonistic interactions between them. Thus, X. frontalis and E. mocsaryi shared the same food source in the flowers of B. excelsa due to differences in their time of visits and non-aggressive way of interacting with the opponent. This result has important implications for pollinating the Brazil nut, and a possible management of X. frontalis and E. mocsaryi, since these two were the most abundant pollinators in the studied locality.A competição entre duas espécies de abelhas por um mesmo tipo de recurso floral pode gerar comportamentos antagônicos entre elas, principalmente, dentro de áreas cultivadas, onde o recurso alimentar é limitado sazonalmente e localmente. No presente trabalho, foi testada a hipótese de antagonismo entre duas espécies de abelhas solitárias da família Apidae, Eulaema mocsaryi (Euglossini e Xylocopa frontalis (Xylocopini em flores da castanheira do Brasil (Bertholletia

  7. Ecophysiological and anatomical characteristics of the subtropical shrub Zanthoxylum acanthopodium (Rutaceae) in conditions of a temperate continental climate (Serbia)

    DEFF Research Database (Denmark)

    Rakic, Tamara; Sinzar-Sekulic, Jasmina; Filipovic, Biljana

    2009-01-01

    The evergreen shrub Zanthoxylum acanthopodium DC. (Rutaceae), originating from warm temperate and subtropical Asia, has existed successfully in the Jevremovac Botanical Garden in Belgrade for more than 80 years. The seasonal pattern of water management in leaves, electrolyte leakage, essential oil...... composition, and leaf anatomy were examined in order to understand the resistance and viability of this subtropical shrub in the temperate continental climate of Belgrade, Serbia....

  8. Impact of ecological diversity on genetic and phytochemical variation injuniperus excelsa from high elevation zones of quetta valley, pakistan

    International Nuclear Information System (INIS)

    Seed, S.; Barozai, M.Y.K.; Ahmed, A.; Tareen, R.B.

    2017-01-01

    Juniperusexcelsa (Cupressaceae) is an evergreen tree and the second most diverse group of the conifers distributed abundantly in high elevation zones of Balochistan. Genetic and phytochemical variations in three naturally occurring populations of J.excelsa were analysed. Genetic variability was assessed by different molecular markers (RAPD, ISSR and URP) with an objective to use genetic diversity as a key to conserve the taxon which is also known as living fossil as dominated in Mesozoic era. Genetic diversity was assessed by polymorphic bands to generate a dendrogram based on UPGMA. Using tested markers, 116 bands were amplified out of which 67 bands were polymorphic with an average value of 8.37 (57%) bands per primer. Based on data, a cluster dendrogram was prepared that exhibited the mean genetic similarity matrix as 0.57 and two major clusters diverge at 0.49. The genetic similarity coefficient among all accessions ranged from 0.35 to 0.90. In phytochemical analysis, total phenolic and flavonoid contents were estimated and compared among all accessions. Ecological characteristics of the study sites were measured to check their impact on genetic and chemical variation. Soil properties were analyzed for Principal Component Analysis. Chemical variation of J. excelsa of three sites revealed by dissimilarity matrix exhibiting genetic distance based on TPC and Flavonoids. Cluster analysis represent two major groups. Mean concentration of TPC and flavonoids were 56+-9.15 and 150+-27.9 mg/g respectively. PCA of soil considered three factors had Eigen values >1 and explain cumulatively 4.60 %, 26.02% and 10.36 % of the variance. First factor was positively correlated with second and fifth, but negatively correlated with other factors. In conclusion, molecular marker profiling together with phytochemical variation of total phenolic and flavonoid content in all accessions of Juniperusexcelsa and impact of ecological diversity on Genetic and chemical variation can be used

  9. Phylogenetic relationships of Vepris (Rutaceae inferred from chloroplast, nuclear, and morphological data.

    Directory of Open Access Journals (Sweden)

    Cynthia M Morton

    Full Text Available The tribe Toddalieae Hook. F. (Rutaceae has been controversial since its inception by Bentham and Hooker. The nine taxa examined, Acronychia J.R. & G.Foster, Diphasia Pierre, Diphasiopsis Mendonca, Fagaropsis Mildbr.ex. Siebenl., Oricia Pierre, Teclea Delile, Toddaliopsis Engl., Toddalia Juss. and Vepris Comm. ex. A. Juss, have been recognized under the tribe Toddalieae or Tribes Acronychia, Phellodendron and Toddalia. More recently Araliopsis Engl., Diphasia, Diphasiopsis, Oricia, Teclea, and Toddaliopsis have been incorporated into the genus Vepris, while Toddalia and Fagaropsis have continued to be recognized as closely related. For this study, sequence data of one non-coding chloroplast region (trnL-F and one nuclear region (ITS and various morphological characters, based on Mziray's taxonomic studies were examined to try to elucidate these relationships. This study found that the taxa Diphasia, Diphasiopsis, Oricia, Teclea, Toddaliopsis, Vepris, Toddalia eugeniifolia Engl. and Toddalia glomerata F. Hoffm. form a monophyletic group. Due to the amount of intrageneric and intraspecific variation, species delimitations were difficult to determine; however, these genera should be united into Vepris. The analyses also confirmed that Toddalia asiatica (L. Lam., Zanthoxylon sp. and Fagaropsis angolensis (Engl. H.M. Gardner are the closest relatives to this group.

  10. Activities of extracts and compounds from Spiranthera odoratissima St. Hil. (Rutaceae) in leaf-cutting ants and their symbiotic fungus

    Energy Technology Data Exchange (ETDEWEB)

    Terezan, Ana Paula; Fernandes, Joao B.; Silva, M. Fatima das Gracas Fernandes da; Vieira, Paulo C. [Universidade Federal de Sao Carlos (UFSCAR), Sao Carlos, SP (Brazil). Dept. de Quimica; Rossi, Raquel Andrade; Almeida, Roberta N.A.; Freitas, Tais Garcia; Bueno, Odair C.; Pagnocca, Fernando C. [Universidade Estadual Paulista (UNESP), Rio Claro, SP (Brazil). Centro de Estudos de Insetos Sociais; Pirani, Jose R., E-mail: djbf@power.ufscar.b [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Biociencias. Dept. de Botanica

    2010-07-01

    The study of the Spiranthera odoratissima St. Hil (Rutaceae) branches extracts led to the isolation of the furoquinoline (dictamine, gamma-fagarine and skimmianine) and 2-arylquinoli-4-one (1-methyl-2-phenylquinoline-4-one) alkaloids and limonoids (limonexic acid and limonin). The compounds 1-methyl-2-phenylquinoline-4-one and limonexic acid were isolated for the first time from the Spiranthera. These furoquinoline and 2-arylquinoli-4-one alkaloids and limonoids showed insecticidal and/or fungicidal activity in the nest of the Atta sexdens rubropilosa. (author)

  11. Estudo quimiossistemático de espécies de Hortia (Rutaceae) e busca de fungicidas à Guignardia citricarpa

    OpenAIRE

    Samya Danielle Lima de Freitas

    2010-01-01

    A classificação do gênero Hortia dentro da família Rutaceae é duvidosa. Hortia, que já foi posicionada em Cusparieae por De Candolle em 1824, na mais recente classificação morfológica de Engler em 1931 pertence a Toddalieae. Mais recentemente da Silva e colaboradores voltam a propor Hortia em Cusparieae. Contudo, o estudo fitoquímico das espécies do gênero Hortia ainda não permitem classificá-la corretamente dentro da família. Desta forma, o estudo fitoquímico de Hortia descrito neste trabalh...

  12. Leaf, stem bark and fruit anatomy of zanthoxylum armatum dc. (rutaceae)

    International Nuclear Information System (INIS)

    Barkatullah, A.; Ibrar, M.; Jelani, G.; Ahmad, I.

    2014-01-01

    Zanthoxylum armatum DC. (Rutaceae) is an important medicinal plant. The present study deals with anatomical exploration of the leaf, stem bark and fruit of this plant. Leaf of Z. armatum is bifacial, compound and punctate with glabrous surfaces having a single layer of epidermis and palisade mesophyll. The leaf has a Palisade ratio ranged from 6.00 to 9.00 (8.2 +- 0.32). Vein islets and vein termination number were 14-21 (16.8 +- 0.64) and 17-21 (19.1 +- 0.43) per mm2 respectively. The vein-islets were quite distinct with squaresh, elongated, polygonal or irregular in shape bounding many forked and unforked vascular branches. Adaxial surface of Z. armatum leaf midrib was planoconvex while the abaxial surface was semicircular in appearance. The diagnostic feature of the leaf was the complete absence of any kind of trichomes or any other appendages. The leaf showed prominent oil cavities. Nine types of stomata with different frequencies and other dimensions were observed. Brachparatetracytic stomata was the most frequent stoma (80%) followed by actinostephanocytic (40%) and then straucytic and brachyparacytic (30%) each. Hemiparacytic and stomatal cluster were the rarely occurring stomata (10% each) present on the lower epidermis of the leaf. Stomatal cluster, which is considered to be a special leaf epidermal feature and reported only in few genera of vascular plants, was also recorded in this plant. Bark and fruit anatomy of Z. armatum showed different tissue arrangement. The seed was non endospermic and contains an elongated embryo. The present study will be helpful in the phylogeny and taxonomic description of this important medicinal plant. (author)

  13. Chemical characterization and radical scavenging activity of leaves of Juniperus foetidisima, J. excelsa and J. communis from Macedonian flora

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    Marija Karapandzova

    2014-12-01

    Full Text Available Chemical characterization of three Juniperus species: J. foetidisima (JF, J. excelsa (JE and J. communis (JC from Macedonian flora enclosed determination of yield and essential oil composition of the oils obtained by hydro-distillation of dried leaves and determination of the content of total phenols and total flavonoids in dried plant material. GC/FID/MS analysis showed mainly monoterpene profile of the JC oil and combined monoterpene/sesquiterpene profile of JF and JE oils. Sesquiterpene cedrol was found as an important constituent of the JF and JE, thus the JF oil was characterized by three main components (a-pinene, limonene and cedrol, in amount up to 67.63%, 27.11% and 33.91%, respectively and JE oil by four components (a-pinene, sabinene, cis-thujone and cedrol, in amount up to 33.83%, 29.49%, 26.20% and 24.44%, respectively. The JC oil was free of cedrol, but contained relatively large sesquiterpene fraction (sesquiterpene hydrocarbons and oxygen containing sesquiterpenes in amounts up to 28.64% and 13.57%, respectively. The JC oil was characterized by three monoterpene components (a-pinene, sabinene and terpinen-4-ol, presented up to 28.68%, 16.27% and 12.16%, respectively. The content of total phenols determined by Folin-Ciocalteu method ranged from 96.18-122.91 mg GAE/g dw (water extraction while the content of total flavonoids ranged from 2.05-11.91 mg CE/g dw (ethanol extraction. Both water and ethanol extracts possessed radical scavenging activity against DPPH radical. Water extracts were more powerful with % of inhibition of DPPH ranging up to 64.52%, 67.40% and 78.23% for water extract (10 mg/ml of JF, JE and JC, respectively. Obtained results showed correlation with the content of total phenols.

  14. Anatomia do lenho de Raulinoa echinata R.S.Cowan (Rutaceae uma espécie endêmica. Wood anatomy of Raulinoa echinata R.S.Cowan (Rutaceae an endemic species.

    Directory of Open Access Journals (Sweden)

    Adriano Antonio DAROSCI

    2012-12-01

    Full Text Available A anatomia vegetal é útil para a caracterização das espécies e do ambiente em que estas vivem. Muitas espécies respondem a algumas características ambientais com alterações na anatomia do lenho, por exemplo, na quantidade de vasos e raios, o que contribui para a sobrevivência no ambiente. Outras respostas anatômicas do lenho, devido às condições fisiológicas-estruturais presentes em diferentes regiões do caule, também são comuns. O presente estudo analisou a anatomia do lenho de Raulinoa echinata, espécie endêmica da mata ciliar do Vale do Itajaí, a fim de buscar alterações do lenho em resposta a algumas características ambientais, além daquelas provocadas pelas diferentes regiões do caule. Para tanto, amostras do lenho foram coletadas em duas regiões: próxima do solo e à altura do peito (1,30 m. Possíveis respostas de R. echinata ao ambiente ripário foram observadas no comprimento e na frequência das fibras, na largura dos raios e no comprimento e na frequência total de vasos, com diferenças significativas, entre as duas regiões, apenas em características quantitativas. A espécie apresentou, ainda, vasos múltiplos tangenciais e em cacho, características até então não descritas para Rutaceae. Não foram vistos na espécie canais traumáticos, característica comum para Rutaceae. Plant anatomy is useful for the characterization of species and their associated environment. Many species, because of habitat features, can show anatomical alterations of wood that permit their survival in the environment, quantity of vessels and rays, for example. Other anatomical alterations caused by structural-physiological conditions present in different regions of the stem are also common. In this study, the anatomy of Raulinoa echinata wood, an endemic riparian forest species of Itajaí Valley, was analyzed in order to identify alterations in wood because of specific habitat features, as well as adaptations in different

  15. Laboratory evaluation of methanolic extract of Atlantia monophylla (Family: Rutaceae against immature stages of mosquitoes and non-target organisms

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    N Sivagnaname

    2004-02-01

    Full Text Available Methanolic extracts of the leaves of Atlantia monophylla (Rutaceae were evaluated for mosquitocidal activity against immature stages of three mosquito species, Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti in the laboratory.Larvae of Cx. quinquefasciatus and pupae of An. stephensi were found more susceptible, with LC50 values of 0.14 mg/l and 0.05 mg/l, respectively. Insect growth regulating activity of this extract was more pronounced against Ae. aegypti, with EI50 value 0.002 mg/l. The extract was found safe to aquatic mosquito predators Gambusia affinis, Poecilia reticulata, and Diplonychus indicus, with the respective LC50 values of 23.4, 21.3, and 5.7 mg/l. The results indicate that the mosquitocidal effects of the extract of this plant were comparable to neem extract and certain synthetic chemical larvicides like fenthion, methoprene, etc.

  16. Four new compounds from the non-polar extract of the plant Amyris brenesii (Rutaceae) from Costa Rica

    International Nuclear Information System (INIS)

    Chavarria, Max; Castro, Victor; Murillo, Renato; Poveda, Luis

    2008-01-01

    The aerial parts of Amyris brenesii (Rutaceae) collected in Rio Cuarto, Grecia, Alajuela (Costa Rica) showed the presence of four new compounds, according to the phytochemical study: the 6-hydroxy-6-O-(3-hydroxymethyl-3-methylallyl)-angelicin 1, 6 - (N-acetyl-2-etanamin) -2,2-dimethyl-2H-chromene 2, the lignans 2,5-dehidrohinokinin 3 and N-acetyl-O-(geranyl)-tyramine 4. Additionally hinokinin lignans were isolated 5 and justicidin E 6, coumarins scopoletin 7 and marmesin 8, the acid 24-moretenoic 9 and O-(3,3-dimethylallyl)-halfordinol 10. The separations were carried out by the application of chromatographic techniques and the elucidation of the structures was carried out with the aid of spectroscopic techniques of nuclear magnetic resonance (NMR) of one and two dimensions. (author) [es

  17. Zanthoxylum bungeanum Maxim. (Rutaceae: A Systematic Review of Its Traditional Uses, Botany, Phytochemistry, Pharmacology, Pharmacokinetics, and Toxicology

    Directory of Open Access Journals (Sweden)

    Mengmeng Zhang

    2017-10-01

    Full Text Available Zanthoxylum bungeanum Maxim. (Rutaceae is a popular food additive and traditional Chinese herbal medicine commonly named HuaJiao in China. This plant is widely distributed in Asian countries. The aim of this paper is to provide a systematic review on the traditional usages, botany, phytochemistry, pharmacology, pharmacokinetics, and toxicology of this plant. Furthermore, the possible development and perspectives for future research on this plant are also discussed. To date, over 140 compounds have been isolated and identified from Z. bungeanum, including alkaloids, terpenoids, flavonoids, and free fatty acids. The extracts and compounds have been shown to possess wide-ranging biological activity, such as anti-inflammatory and analgesic effects, antioxidant and anti-tumor effects, antibacterial and antifungal effects, as well as regulatory effects on the gastrointestinal system and nervous system, and other effects. As a traditional herbal medicine, Z. bungeanum has been widely used to treat many diseases, especially digestive disorders, toothache, stomach ache, and diarrhea. Many traditional usages of this plant have been validated by present investigations. However, further research elucidating the structure-function relationship among chemical compounds, understanding the mechanism of unique sensation, as well as exploring new clinical effects and establishing criteria for quality control for Z. bungeanum should be further studied.

  18. Effect of irrigation and fertilization on crop yield and fruit quality of the Tahiti lime Citrus latifolia Tanaka (Rutaceae

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    Diana Dorado Guerra

    2015-01-01

    Full Text Available In a seven years old citrus grove of Tahiti lime Citrus latifolia Tanaka (Rutaceae located in El Espinal, Tolima, Colombia, which has a predominantly negative water balance throughout the year, we evaluated the effect of irrigation and fertilization on yield and fruit quality in 2009 and 2011. Trees were subjected to three levels of water based on the evapotranspiration reference (ETo: L1=100 %, L2=70 % and L3=50 %. We applied three nitrogen treatments: N1=nitrogen required by the nutritional balance, N2=twice the level of nitrogen used in N1, and N3=fertilizer application used by the common farmer. We evaluated the performance, fruit weight, polar and equatorial diameter of fruit, shell thickness, weight and percentage of juice, acidity, soluble solids and vitamin C. The experiment was conducted under a randomized complete block design in a split plot arrangement; the variance and means of the data were statistically analyzed with SAS. And optimal response is between irrigation and fruit quality interaction was obtained with irrigation L1 and fertilization N2. We obtained the highest values in the response variables when the highest amount of water was applied L1, regardless of fertilizer applications, indicating that a lower water supply not only affects the performance of the plant, but also the quality of the fruit, minimizing market opportunities.

  19. Characteristic differences in essential oil composition of six Zanthoxylum bungeanum Maxim. (Rutaceae) cultivars and their biological significance.

    Science.gov (United States)

    Liu, Shu-Ming; Wang, Shi-Jun; Song, Si-Yao; Zou, Yong; Wang, Jun-Ru; Sun, Bing-Yin

    Great variations have been found in composition and content of the essential oil of Zanthoxylum bungeanum Maxim. (Rutaceae), resulting from various factors such as harvest time, drying and extraction methods (Huang et al., 2006; Shao et al., 2013), solvent and herbal parts used (Zhang, 1996; Cao and Zhang, 2010; Wang et al., 2011). However, in terms of artificial introduction and cultivation, there is little research on the chemical composition of essential oil extracted from Z. bungeanum Maxim. cultivars, which have been introduced from different origins. In this study, the composition and content of essential oil from six cultivars (I-VI) have been investigated. They were introduced and cultivated for 11 years in the same cultivation conditions. Cultivars were as followings: Qin'an (I) cultivar originally introduced from Qin'an City in Gansu Province; Dahongpao A (II) from She County in Hebei Province; Dahongpao B (III) from Fuping County; Dahongpao C (IV) from Tongchuan City; Meifengjiao (V) from Feng County; and, Shizitou (VI) from Hancheng City, in Shaanxi Province, China. This research is expected to provide a theoretical basis for further introduction, cultivation, and commercial development of Z. bungeanum Maxim.

  20. Zanthoxylum bungeanum Maxim. (Rutaceae): A Systematic Review of Its Traditional Uses, Botany, Phytochemistry, Pharmacology, Pharmacokinetics, and Toxicology.

    Science.gov (United States)

    Zhang, Mengmeng; Wang, Jiaolong; Zhu, Lei; Li, Tao; Jiang, Weidong; Zhou, Juan; Peng, Wei; Wu, Chunjie

    2017-10-18

    Zanthoxylum bungeanum Maxim. (Rutaceae) is a popular food additive and traditional Chinese herbal medicine commonly named HuaJiao in China. This plant is widely distributed in Asian countries. The aim of this paper is to provide a systematic review on the traditional usages, botany, phytochemistry, pharmacology, pharmacokinetics, and toxicology of this plant. Furthermore, the possible development and perspectives for future research on this plant are also discussed. To date, over 140 compounds have been isolated and identified from Z. bungeanum , including alkaloids, terpenoids, flavonoids, and free fatty acids. The extracts and compounds have been shown to possess wide-ranging biological activity, such as anti-inflammatory and analgesic effects, antioxidant and anti-tumor effects, antibacterial and antifungal effects, as well as regulatory effects on the gastrointestinal system and nervous system, and other effects. As a traditional herbal medicine, Z. bungeanum has been widely used to treat many diseases, especially digestive disorders, toothache, stomach ache, and diarrhea. Many traditional usages of this plant have been validated by present investigations. However, further research elucidating the structure-function relationship among chemical compounds, understanding the mechanism of unique sensation, as well as exploring new clinical effects and establishing criteria for quality control for Z. bungeanum should be further studied.

  1. Efficacy of cryotherapy plus topical Juniperus excelsa M. Bieb cream versus cryotherapy plus placebo in the treatment of Old World cutaneous leishmaniasis: A triple-blind randomized controlled clinical trial.

    Science.gov (United States)

    Parvizi, Mohammad Mahdi; Handjani, Farhad; Moein, Mahmoodreza; Hatam, Gholamreza; Nimrouzi, Majid; Hassanzadeh, Jafar; Hamidizadeh, Nasrin; Khorrami, Hamid Reza; Zarshenas, Mohammad Mehdi

    2017-10-01

    Cutaneous leishmaniasis is one of the highly prevalent endemic diseases in the Middle East and North Africa. Many treatment modalities have been recommended for this condition but success rates remain limited. Herbal remedies have also been used for treatment but evidence-based clinical trials with these products are sparse. In-vitro and in-vivo studies have shown the anti-leishmanial and curative effects of extract of fruits and leaves of Juniperus excelsa (J. excelsa). The aim of this study was to determine the efficacy of topical J. excelsa M. Bieb extract as an adjuvant to cryotherapy for the treatment of human CL. This study was designed as a two-arm triple-blind randomized placebo-controlled clinical trial using a parallel design. Seventy-two patients with clinical diagnosis of CL confirmed by leishmania smears were allocated to receive either a topical formulation of leaf of J. excelsa extract (group A) or placebo (group B) for 3 months. Both groups received cryotherapy as baseline standard treatment. Patients were evaluated before and weekly after the intervention was initiated until complete cure. Overall, 82% of patients in group A, experienced complete cure and 9% of them had partial cure. On the other hand, 34% in group B reported complete cure, while 14% of them had partial cure at the end of treatment protocol with a significant difference between the two groups (Pcryotherapy for accelerating the time to cure in addition to increasing the complete cure rate in CL. ClinicalTrials.gov IRCT2015082523753N1.

  2. Extrativismo, cultivo e privatização do jaborandi (Pilocarpus microphyllus Stapf ex Holm.; Rutaceae no Maranhão, Brasil Extractivism, cultivation and privatization of jaborandi (Pilocarpus microphyllus Stapf ex Holm.; Rutaceae in Maranhão, Brazil

    Directory of Open Access Journals (Sweden)

    Claudio Urbano B. Pinheiro

    2002-04-01

    Full Text Available Um dos processos associados com a expansão de fronteiras em países em desenvolvimento consiste na privatização de recursos naturais, anteriormente de uso comum por populações tradicionais. Em geral, a privatização não só conduz à degradação dos recursos como também ao empobrecimento de comunidades locais dependentes desses recursos. Um exemplo desta transformação está no Estado do Maranhão, especialmente em uma região conhecida como "Pré-Amazônia", onde o jaborandi (Pilocarpus microphyllus Stapf ex Holm.; Rutaceae, um recurso natural que beneficiava milhares de pessoas da zona rural, foi gradativamente privatizado. A expansão do mercado de pilocarpina (um alcalóide de extenso uso em oftalmologia nos últimos 30 anos, provocou crescimento descontrolado no extrativismo de folhas de jaborandi, conduzindo ao esgotamento e à ameaça de extinção das populações naturais deste recurso vegetal. Paralelamente, esta pressão sobre as populações naturais acelerou o processo de domesticação desta espécie e a sua privatização pela indústria farmacêutica. Este estudo teve como objetivo principal avaliar a exploração e cultivo do jaborandi no Maranhão, buscando o esclarecimento sobre os benefícios e malefícios econômicos, sociais e ambientais decorrentes da sua exploração. Os resultados obtidos mostram que, historicamente, não houve nenhum benefício significativo compartilhado entre a indústria farmacêutica e as populações envolvidas no extrativismo do jaborandi no Maranhão.A process associated with frontier expansion in developing countries is the privatization of natural resources formerly held in common by traditional populations. An example of this transformation comes from Maranhão State, in a region of mid-northern Brazil known as "Pre-Amazônia" (Pre-Amazon, where jaborandi (Pilocarpus microphyllus Stapf ex Holm.; Rutaceae, a plant resource that used to benefit thousands of rural poor, is being

  3. Hypoglycemic and hypolipidemic effects of methanol seed extract of Citrus paradisi Macfad (Rutaceae) in alloxan-induced diabetic Wistar rats.

    Science.gov (United States)

    Adeneye, A A

    2008-01-01

    Alcohol decoction of Citrus paradisi Macfad (Rutaceae) seed is reputed for the local management of array of human diseases including, anemia, diabetes mellitus and obesity by some Yoruba herbalists (SouthWest, Nigeria). Despite its historic use, scientific evaluation of its folkloric use in the management of diabetes mellitus is scarce. The present study was designed at investigating the glucose and lipid lowering effects of methanol seed extract of Citrus paradisi Macfad (MECP) in alloxan-induced diabetic rats. In addition, the phytochemical analysis of the extract was also conducted using standard procedures. Young adult, male, alloxan-induced diabetic rats were randomly divided into groups I - VI with 12 rats in each group. Group I rats were the normal untreated rats while group II rats served as the diabetic untreated rats while Rats in groups III - VI served as diabetic rats treated with 100, 300 and 600 mg/kg/day MECP and 20 mg/kg/ day metformin, respectively, for 30 days. On the 15th and respectively, 31st day, blood samples from the fasted rats were obtained for fasting plasma glucose (FPG), plasma triglycerides (TG), total cholesterol (TC), high density lipoprotein- cholesterol (HDL-c), low density lipoprotein-cholesterol (LDL-c) and very low density lipoprotein-cholesterol (VLDL-c) from the sacrificed rats. Oral treatment with 100 - 600 mg/kg/day MECP, for 30 days, resulted in significant (p extract also caused significant (p extract could be due to any or a combination of these phytochemical constituents. Results of this study lend support to the traditional use of grapefruit seeds in the management of type 1 diabetic patients and may suggest a role in orthodox management of the disease.

  4. Isolation and structural elucidation of secondary metabolites from plants of the Rutaceae family, Rubiaceae, Euphorbiaceae and Salicaceae

    International Nuclear Information System (INIS)

    Calderon Castro, Carlos

    2014-01-01

    A phytochemical study was conducted of the Zuelania guidonia plants (Salicaceae), croton ovalifolius (Euphorbiaceae) erythrochiton gymnanthus (Rutaceae) and Faramea occidentalis (Rubiaceae). Purification of the compounds was carried out using chromatographic techniques while structural elucidation was performed by experiments using nuclear magnetic resonance (NMR) and mass spectrometry (MS). Of Z. guidonia has been possible the purification and structural elucidation of 22 compounds (Z1-Z22), two labdane type diterpenes and 20 clerodane-type diterpenes. The clerodanes have presented 16 innovative structure, highlighting the presence of a group of 3,6-dihydro -1.2-dioxin and xylose group in some of them. In addition, 11 of the clerodanes were evaluated with cytotoxicity assays in three cancer cell lines CCRF-CEM (acute lymphoblastic leukemia), CEM-ADR5000 (acute lymphoblastic leukemia resistant to doxorubicin) MIA-Paca-2 (metastatic pancreas) and a line of healthy cells PBMC (peripheral blood mononuclear cells). The Z4, Z6 and Z15 compounds stood out as the most cytotoxic, particularly against CCRF-CEM cells with IC 50 values between 1.6 and 2.5 μM. Seven compounds identified as glutarimide alkaloids (C1-C7) were isolated and elucidated, five of which have presented a novel structure from C. ovalifolius. Three compounds (E1-E3) that are triterpenes derivatives of known structure sitosterol, were isolated and elucidated from E. gymnanthus plant. From F. occidentalis was obtained the structure of a pure compound (F1], which is a flavonoid of known structure. (author) [es

  5. Sobrevivência e Frutificação de Bertholletia excelsa Bonpl. em Áreas Desmatadas em Oriximiná, Pará

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    Ricardo Scoles

    Full Text Available RESUMO Analisaram-se as condições biológicas (sobrevivência, reprodução, rebrotação e estrutura de populações de castanheira (Bertholletia excelsa Bonpl. nas margens de estradas em áreas desmatadas (Oriximiná, Pará. Identificaram-se 441 árvores de castanheira (Diâmetro à Altura do Peito – DAP > 10 cm. De cada árvore, coletaram-se as coordenadas geográficas, dados dendrométricos e registrou-se o estado vital e reprodutivo, assim como evidências de perturbação e rebrotamento. Cerca de 75% das castanheiras foram encontradas em estado morto. A estrutura populacional das árvores vivas é envelhecida (DAP médio, 159 ± 0,55 cm, com baixa presença de jovens improdutivos (4,6%. Pouco mais da metade das castanheiras adultas sobreviventes (DAP > 40 cm apresentou evidências de frutificação. As árvores com frutos apresentaram uma área de copa significativamente maior que as improdutivas. São necessárias medidas urgentes de conservação e reflorestamento da população de castanheiras na região do estudo, em conformidade da legislação ambiental vigente.

  6. Melicope stonei, section Pelea (Rutaceae, a new species from Kaua‘i, Hawaiian Islands: with notes on its distribution, ecology, conservation status, and phylogenetic placement

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    Kenneth R. Wood

    2017-08-01

    Full Text Available Melicope stonei K.R. Wood, Appelhans & W.L. Wagner (section Pelea, Rutaceae, a new endemic tree species from Kaua‘i, Hawaiian Islands, is described and illustrated with notes on its distribution, ecology, conservation status, and phylogenetic placement. The new species differs from its Hawaiian congeners by its unique combination of distinct carpels and ramiflorous inflorescences arising on stems below the leaves; plants monoecious; leaf blades (5–8–30 × (4–6–11 cm, with abaxial surface densely tomentose, especially along midribs; and very long petioles of up to 9 cm. Since its discovery in 1988, 94 individuals have been documented and are confined to a 1.5 km2 region of unique high canopy mesic forest. Melicope stonei represents a new Critically Endangered (CR single island endemic species on Kaua‘i.

  7. Iridoid Glucosides from Picconia excelsa

    DEFF Research Database (Denmark)

    Damtoft, Søren; Franzyk, Henrik; Jensen, Søren Rosendal

    1997-01-01

    constituents were secoxyloganin, 8-epi-kingiside, 8-epi-kingisidic, oleoside 11-methyl ester, excelsioside, ligstroside and loganic acid together with the new compounds ketologanic acid, 6 beta-hydroxy-7-epi-loganin and picconiosides II-V, the latter four being esters of loganin and menthiafolic, foliamenthic...

  8. Tracing the temporal and spatial origins of island endemics in the Mediterranean region: a case study from the citrus family (Ruta L., Rutaceae).

    Science.gov (United States)

    Salvo, Gabriele; Ho, Simon Y W; Rosenbaum, Gideon; Ree, Richard; Conti, Elena

    2010-12-01

    Understanding the origin of island endemics is a central task of historical biogeography. Recent methodological advances provide a rigorous framework to determine the relative contribution of different biogeographic processes (e.g., vicariance, land migration, long-distance dispersal) to the origin of island endemics. With its complex but well-known history of microplate movements and climatic oscillations, the Mediterranean region (including the Mediterranean basin and Macaronesia) provides the geographic backdrop for the diversification of Ruta L., the type genus of Rutaceae (citrus family). Phylogenetic, molecular dating, and ancestral range reconstruction analyses were carried out to investigate the extent to which past geological connections and climatic history of the Mediterranean region explain the current distribution of species in Ruta, with emphasis on its island endemics. The analyses showed that Ruta invaded the region from the north well before the onset of the Mediterranean climate and diversified in situ as the climate became Mediterranean. The continental fragment island endemics of the genus originated via processes of land migration/vicariance driven by connections/disconnections between microplates, whereas the oceanic island endemics were the product of a single colonization event from the mainland followed by in situ diversification. This study emphasizes the need for an integrative, hypothesis-based approach to historical biogeography and stresses the importance of temporary land connections and colonization opportunity in the biotic assembly of continental fragment and oceanic islands, respectively.

  9. Desenvolvimento de Rhapis excelsa (Thunberg Henry Ex. Rehder (Palmeira-Ráfia: Influência da altura do recipiente na formação de mudas Development of Rhapis excelsa (Thunberg Henry Ex. Rehder (Palm-Ráfia: influence of the height of the recipient in the formation of seedlings

    Directory of Open Access Journals (Sweden)

    Petterson Baptista da Luz

    2006-02-01

    Full Text Available Rhapis excelsa, originária do sul da China, é uma das palmeiras ornamentais mais cultivadas no mundo, principalmente em vaso. Objetivou-se com esta pesquisa investigar a influência da altura do vaso no desenvolvimento da espécie. Para tanto, mudas de dois anos de idade, apresentando 10 cm de altura e sistema radicular com 10 cm de comprimento, foram transplantadas para recipientes de PVC com 10 cm de diâmetro e 20, 25, 30, 35 e 40 cm de altura, que se constituíram em tratamentos. O substrato utilizado foi uma mistura de casca de pinho, vermiculita e solo argiloso (2:1:1, adubado com 45 g de Osmocote (15:10:10. Os parâmetros avaliados foram: altura da planta, diâmetro do colo, número de folhas, número de perfilhos emitidos, avaliados aos 6, 12 e 18 meses após a instalação do experimento. Aos 18 meses foram tomados também o peso da matéria seca da parte aérea e o peso da matéria seca das raízes. O delineamento experimental foi inteiramente casualizado, com cinco repetições com duas plantas. Houve diferença significativa para a variável altura de plantas, aos 6 meses, sendo que o recipiente de 40 cm de altura apresentou o pior resultado. Não houve diferença significativa entre as variáveis analisadas aos 12 meses de investigação. Já aos 18 meses, houve diferenças significativas entre os tratamentos para as variáveis altura da planta e diâmetro do colo, sendo o recipiente de 40 cm inferior aos demais em relação à altura da planta e o de 25 cm superior quanto ao diâmetro do colo. A utilização do recipiente com 25 cm de altura revelou-se interessante pela qualidade das plantas formadas e em relação a aspectos econômicos da produção.Rhapis excelsa is one the most important ornamental palm cultivated in the world, mainly as vase plant, being indigenous to China. The aim of this research was to investigate the influence of vase height on the development of the species. Two years-old seedlings with 10 cm height

  10. The Effect of Seasonal Ambient Temperatures on Fire-Stimulated Germination of Species with Physiological Dormancy: A Case Study Using Boronia (Rutaceae)

    Science.gov (United States)

    Auld, Tony D.; Keith, David A.; Hui, Francis K. C.; Ooi, Mark K. J.

    2016-01-01

    Dormancy and germination requirements determine the timing and magnitude of seedling emergence, with important consequences for seedling survival and growth. Physiological dormancy is the most widespread form of dormancy in flowering plants, yet the seed ecology of species with this dormancy type is poorly understood in fire-prone vegetation. The role of seasonal temperatures as germination cues in these habitats is often overlooked due to a focus on direct fire cues such as heat shock and smoke, and little is known about the combined effects of multiple fire-related cues and environmental cues as these are seldom assessed in combination. We aimed to improve understanding of the germination requirements of species with physiological dormancy in fire-prone floras by investigating germination responses across members of the Rutaceae from south eastern Australia. We used a fully factorial experimental design to quantify the individual and combined effects of heat shock, smoke and seasonal ambient temperatures on germination of freshly dispersed seeds of seven species of Boronia, a large and difficult-to-germinate genus. Germination syndromes were highly variable but correlated with broad patterns in seed morphology and phylogenetic relationships between species. Seasonal temperatures influenced the rate and/or magnitude of germination responses in six species, and interacted with fire cues in complex ways. The combined effects of heat shock and smoke ranged from neutral to additive, synergistic, unitive or negative and varied with species, seasonal temperatures and duration of incubation. These responses could not be reliably predicted from the effect of the application of single cues. Based on these findings, fire season and fire intensity are predicted to affect both the magnitude and timing of seedling emergence in wild populations of species with physiological dormancy, with important implications for current fire management practices and for population

  11. Phylogeny, evolutionary trends and classification of the Spathelia–Ptaeroxylon clade: morphological and molecular insights

    Science.gov (United States)

    Appelhans, M. S.; Smets, E.; Razafimandimbison, S. G.; Haevermans, T.; van Marle, E. J.; Couloux, A.; Rabarison, H.; Randrianarivelojosia, M.; Keßler, P. J. A.

    2011-01-01

    Background and Aims The Spathelia–Ptaeroxylon clade is a group of morphologically diverse plants that have been classified together as a result of molecular phylogenetic studies. The clade is currently included in Rutaceae and recognized at a subfamilial level (Spathelioideae) despite the fact that most of its genera have traditionally been associated with other families and that there are no obvious morphological synapomorphies for the clade. The aim of the present study is to construct phylogenetic trees for the Spathelia–Ptaeroxylon clade and to investigate anatomical characters in order to decide whether it should be kept in Rutaceae or recognized at the familial level. Anatomical characters were plotted on a cladogram to help explain character evolution within the group. Moreover, phylogenetic relationships and generic limits within the clade are also addressed. Methods A species-level phylogenetic analysis of the Spathelia–Ptaeroxylon clade based on five plastid DNA regions (rbcL, atpB, trnL–trnF, rps16 and psbA–trnH) was conducted using Bayesian, maximum parsimony and maximum likelihood methods. Leaf and seed anatomical characters of all genera were (re)investigated by light and scanning electron microscopy. Key Results With the exception of Spathelia, all genera of the Spathelila–Ptaeroxylon clade are monophyletic. The typical leaf and seed anatomical characters of Rutaceae were found. Further, the presence of oil cells in the leaves provides a possible synapomorphy for the clade. Conclusions The Spathelia–Ptaeroxylon clade is well placed in Rutaceae and it is reasonable to unite the genera into one subfamily (Spathelioideae). We propose a new tribal classification of Spathelioideae. A narrow circumscription of Spathelia is established to make the genus monophyletic, and Sohnreyia is resurrected to accommodate the South American species of Spathelia. The most recent common ancestor of Spathelioideae probably had leaves with secretory cavities

  12. Spatial distribution structure and volume of Colombian black oak forest (Colombobalanus excelsa (Lozano, Hern. Cam. & Henao, J.E. Nixon & Crepet National Natural Park Cueva de los Guácharos

    Directory of Open Access Journals (Sweden)

    David Eduardo Dávila

    2012-12-01

    Full Text Available The spatial distribution of Colombobalanus excelsa forests in the Cueva de los Guácharos Natural National Park and its buffer zone was determined. The forest’s structural parameters were determined by conducting a stratified forest inventory that consisted of four plots of 0.5 ha distributed in two strata. The first stratum was located in the park and the second in its buffer zone. Each strip consisted of plots of 20 x 50 m within which individuals with diameters at breast height = 10 cm DBH were measured for total height, crown diameter and the condition of each tree. Within each strip a 10 x 10 m subplot was used to assess individuals with DBH = 10 cm and heights greater than 3 m. In addition the number of seedlings of height = 0.3 m were counted in subplots of 5 x 5 m. Models were generated to estimate the height and volume as a function of DBH. We report a total of eight natural stands of black oak reaching 2000 ha of which 28.3 ha were found within the park. We report a density of 281.7 trees ha-1 with a basal area of 52.33 m2 ha-1 and a volume of 761.65 m3 ha-1. The form-factor for the species was of 0.76041. Six models were fitted to estimate the height and six for volume adjustments of 0.90 and 0.988, respectively.

  13. Constituintes químicos e estudos toxicológicos do óleo essencial extraído das folhas de Citrus limon Burn (Rutaceae

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    L.M.L. Campelo

    2013-01-01

    Full Text Available A caracterização química do óleo essencial de folhas de Citrus limon (Rutaceae resultou na identificação de mistura de monoterpenos (limoneno, linalol, cis-óxido de limoneno, trans-óxido de limoneno, citronelal, neral, geranial, nerol e acetato de geranil. As estruturas dos compostos do óleo essencial foram identificadas por GC/MS, por comparação com dados da literatura. Os efeitos da administração crônica oral do óleo essencial de folhas de Citrus limon foram investigados sobre parâmetros bioquímicos e hematológicos em camundongos Swiss machos. Os animais (n = 10/grupo foram tratados por via oral diariamente durante 30 dias com óleo essencial de folhas de Citrus limon, nas doses de 50, 100 ou 150 mg kg-1 de massa corporal e os parâmetros bioquímicos e hematológicos avaliados. O tratamento não causou nenhuma morte ou toxicidade nos animais. A administração do óleo essencial não alterou os parâmetros bioquímicos e hematológicos e a massa dos órgãos, exceto por diminuição de 21 e 11% em uréia e ácido úrico, respectivamente, e 9%, nos níveis plasmáticos de aspartato transaminase (AST. Para os parâmetros hematológicos, houve pequenas mudanças nas contagens de neutrófilos, linfócitos, eosinófilos e monócitos, mas estes não foram diferentes dos valores de referência. Além disso, houve diminuição significativa nos triglicerídeos detectado nos animais tratados com dose de 150 mg kg-1 de óleo essencial. Em conclusão, a administração crônica de óleo essencial não induziu nenhum efeito de risco na maioria dos parâmetros bioquímicos e hematológicos estudados em camundongos Swiss machos. No entanto, a diminuição dos níveis de uréia e ácido úrico em doses elevadas, sugere um possível efeito de insuficiência renal e aumento no teor de AST, sugerindo possível sobrecarga hepática que deve ser investigada com mais detalhe.

  14. Dendrometria de espécies nativas em plantios homogêneos no estado de Roraima: andiroba (Carapa guianensis Aubl, castanha-do-Brasil (Bertholletia excelsa Bonpl., ipê-roxo (Tabebuia avellanedae Lorentz ex Griseb e jatobá (Hymenaea courbaril L. Dendrometry of native species in homogeneous stands in the Roraima state: andiroba (Carapa guianensis Aubl, castanha-do-brasil (Bertholletia excelsa Bonpl, ipê-roxo (Tabebuia avellanedae Lorentz ex Griseb and jatobá (Hymenaea courbaril L.

    Directory of Open Access Journals (Sweden)

    Helio Tonini

    2005-09-01

    Full Text Available Estudou-se o crescimento e a seleção de equações para quatro espécies florestais nativas visando identificar espécies promissoras para o plantio em programas de reflorestamentos e em sistemas agroflorestais no Estado de Roraima. O crescimento da andiroba (Carapa guianensis Aubl., da castanha-do-brasil (Bertholletia excelsa Bonpl., do ipê-roxo (Tabebuia avellanedae Lorentz ex Griseb. e do jatobá (Hymenaea courbaril L., aos sete anos de idade, mostrou-se promissor atingindo incrementos médios anuais em volume comercial de 6.3; 14.6; 6.0 e 2.3 m³.ha-1.ano-1, respectivamente. Em relação ao crescimento em diâmetro, todas as espécies apresentaram incrementos médios anuais em diâmetro maiores do que 1 cm, sendo superiores aos observados para árvores crescendo em florestas naturais. A análise estatística, indicou a equação hipsométrica de Prodan como a de melhor ajuste para estimar a altura em função do diâmetro para as quatro espécies analisadas. No entanto, a análise gráfica indicou que a forma da curva altura/diâmetro variou com a espécie sendo necessário o ajuste em separado. O ajuste de equações de volume comercial com casca e fator de forma comercial mostraram ser necessário o ajuste de diferentes equações em função da espécie. A análise gráfica das curvas de volume comercial e fator de forma indicaram que as espécies diferiram em ambos os parâmetros, indicando que a utilização de um fator de forma médio para todas as espécies deve ser evitado, como forma de aumentar a precisão nas estimativas volumétricas.The growth and selection of equations for four native forest species was studied aiming to identify promising species for homogeneous stands, and agroflorestry systems in the Roraima state. The growth of andiroba (Carapa guianensis Aubl., castanha-do-brasil (Bertholletia excelsa Bonpl., ipê-roxo (Tabebuia avellanedae Lorentz ex Griseb and jatobá (Hymenaea courbaril L. to the seven years of age

  15. [A new taxonomic system of the genus Murraya (Rutaceae) based on integration of morphology-based taxonomy and chemotaxonomy; and a philological survey on M. exotica in view of the relationship between Okinawa and China].

    Science.gov (United States)

    Kinoshita, Takeshi

    2014-01-01

    This review concerns the taxonomic status of the genus Murraya in tribe Clausenae, subfamily Aurantioideae, family Rutaceae, and presents a new system integrating both morphology-based taxonomy and chemotaxonomy. This genus has been morphologically divided into the sects Murraya and Bergera. This dichotomy is justified by the noticeable difference of secondary metabolites with 3-prenylindoles in Murraya and carbazoles in Bergera. As for other metabolites of genus Murraya, coumarins are found in both sects, but differ clearly in types; 8-prenylcoumarins occur throughout the sect Murraya whereas geranylated furocoumarins are known from some species of the sect Bergera. As far as chemical properties are concerned, sect Bergera is much closer to genus Clausena than sect Murraya, suggesting the dichotomy of genus Murraya to be generic rather than sectional. 8-Prenylcoumarins characterizing sect Murraya play a decisive role in the distinction of M. exotica from M. paniculata that occurs most widely in subtropical and tropical Asia and is well known for morphologic as well as chemical diversity. Though the morphological difference between the two species is slight only in leaves and leaflets, the distinction is well substantiated by the following chemical feature: 7-OMe-8-prenylcoumarins occur in M. exotica whereas 5,7-di-OMe-8-prenylcoumarins in M. paniculata. Sect Murraya has a very close relation to genus Merrillia that is chemically characterized by similar types of 8-prenylcoumarins, and is also related to a certain extent to genus Micromelum. M. exotica is philologically surveyed in view of the delicate relationships between Okinawa, the only habitat of this plant in Japan, and China in order to clarify its historical background.

  16. Avaliação da atividade antioxidante e alelopática do extrato etanólico e frações das cascas do caule de Zanthoxylum rhoifolium Lam., Rutaceae

    Directory of Open Access Journals (Sweden)

    Joelle de Melo Turnes

    2014-08-01

    Full Text Available Este trabalho teve como objetivo o estudo das atividades antioxidante e alelopática das cascas do caule de Zanthoxylum rhoifolium Lam., Rutaceae, de modo a conduzir à descoberta de substâncias biologicamente ativas. O material vegetal foi submetido à extração etanólica e este extrato foi fracionado obtendo as frações (hexano, clorofórmio, acetato de etila e hidroalcoólica. Para a avaliação da atividade antioxidante, empregaramse os métodos de redução do complexo fosfomolibdênio, de redução do radical DPPH e das substâncias reativas ao acido tiobarbitúrico (TBARS. Quanto à alelopatia, as amostras foram testadas em quatro concentrações sobre a germinação e o desenvolvimento de radícula e hipocótilo das sementes de Lactuca sativa. As amostras evidenciaram atividade antioxidante significativa frente ao método de redução do complexo fosfomolibdênio quando comparada à rutina, e do TBARS quando comparado ao BHT, assim como a atividade alelopática, uma vez que estimularam tanto a germinação como o crescimento das sementes. A fração clorofórmica e acetato de etila demonstraram melhor potencial antioxidante com 204,17% e 127,11% em relação à rutina no método de formação do complexo fosfomolibdênio, e o extrato bruto e a fração hexano com 64,2% e 60,9%, em relação ao BHT, no método TBARS. No ensaio alelopático, destaca-se a fração clorofórmica, pois foi a única amostra que estimulou o crescimento do hipocótilo e radícula na maioria das concentrações, variando de 41 a 144%, e a fração acetato de etila que apresentou a maior porcentagem de estímulo nesse bioensaio, demonstrando estímulo de 274% do crescimento do hipocótilo. Este é o primeiro trabalho que demonstra a atividade antioxidante e alelopática de Z. rhoifolium.

  17. Secondary metabolites from Esenbeckia almawillia Kaastra (Rutaceae)

    International Nuclear Information System (INIS)

    Barros-Filho, Bartholomeu A.; Nunes, Fatima M.; Oliveira, Maria da Conceicao F. de; Andrade-Neto, Manoel; Mattos, Marcos C. de; Barbosa, Francisco G.; Mafezoli, Jair; Pirani, Jose R.

    2007-01-01

    The phytochemical investigation of the roots of E. almawillia is reported for the first time. Chromatographic fractionation of the methanol extract allowed the isolation of the alkaloids 3,3-diisopentenyl-N-methyl-2,4-quinoldione (1), maculine (2) and 3'-methoxygraveoline (3), (E)-N-isobutyl-3- methoxy-4,5-methylenedioxicinnamoyl amide (4), the flavones gardenine B (5) and nevadensin (6), and the sesquiterpene intermediol (7). Structure elucidation was based on the analysis of their spectrometric data (uni- and bidimensional 1 H and 13 C NMR, MS and IR) and comparison with literature data. Compounds 3-7 are being reported as constituents of Esenbeckia species for the first time. (author)

  18. Evidências científicas para a indicação popular de algumas espécies da família Rutaceae no tratamento de doenças respiratórias na região Sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    Renata Correia Campello PRUDENTE, Regina Braga de MOURA

    2013-12-01

    Full Text Available A utilização de plantas medicinais como forma de tratamento vem sendo bastante empregada pela população brasileira, sendo a maioria através do uso empírico. Diversas patologias têm sido tratadas através da utilização popular de plantas medicinais, sendo uma das principais as doenças do trato respiratório. O objetivo do presente trabalho foi verificar se existem evidências científicas para o uso popular das espécies de Rutaceae: Citrusaurantiifolia (Christm. Swingle, Citrus aurantiumL., Citrus limonum Risso, Citrus medica L., Citrussinensis Osbeck e Ruta graveolens L. na regiãoSudeste, para problemas respiratórios. Foram considerados artigos sobre estudos farmacológicos envolvendo doenças respiratórias, assim como sobre a ação anti-inflamatória e anti-bacteriana das espécies. A revisão mostrou que a presença de flavonóides, hidrocarbonetos terpênicos e monoterpênicos nos extratos e nos óleos essenciais dessas plantas causam um efeito anti-inflamatório, através da inibição de mediadores pró-inflamatórios iNOS e COX-2; e atividade anti-bacteriana, analisada por meio do método de difusão em disco. O trabalho mostrou que apesar do intenso uso da população da região Sudeste do Brasil de espécies medicinais da família Rutaceae para tratamento de doenças do trato respiratório, ainda existem poucos estudos que evidenciem cientificamente as ações farmacológicas, justificando assim a grande necessidade de estudos clínicos para tratamentos feitos com a utilização das espécies estudadas.

  19. Phytochemical and chemosystematic studies of Euxylophora paraensis (Rutaceae); Fitoquimica e quimiossistematica de Euxylophora paraensis (Rutaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Isidoro, Marsele Machado; Silva, Maria Fatima das Gracas Fernandes da; Fernandes, Joao Batista; Vieira, Paulo Cezar [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica; Arruda, Alberto C.; Silva, Sebastiao da Cruz, E-mail: dmfs@ufscar.br [Universidade Federal do Para (UFPA), Belem, PA (Brazil). Fac. de Quimica

    2012-07-01

    Phytochemical studies of the leaves and stem have led to the identification of the known coumarins isooxypeucedanin, oxypeucedanin hydrate, xanthotoxin, isopimpinellin, 8-methoxymarmesin and marmesin, flavonoids quercetin-3-O-{alpha}-L-rhamnopyranoside, myricetin-3-O-{alpha}-L-rhamnopyranoside and hesperidin, alkaloids skimmianine and N-methylflindersine and limonoid limonin. The compounds isolated and the chemical profile of Euxylophora obtained from the literature clearly indicate its phytochemical affinities with other Rutoideae species. (author)

  20. Secondary metabolites from Esenbeckia almawillia Kaastra (Rutaceae); Metabolitos secundarios de Esenbeckia almawillia Kaastra (Rutaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Barros-Filho, Bartholomeu A.; Nunes, Fatima M.; Oliveira, Maria da Conceicao F. de; Andrade-Neto, Manoel; Mattos, Marcos C. de [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica]. E-mail: mcfo@ufc.br; Barbosa, Francisco G. [Universidade Federal do Ceara (UFC), Itapipoca, CE (Brazil). Faculdade de Educacao; Mafezoli, Jair [Universidade de Fortaleza, CE (Brazil). Curso de Farmacia; Pirani, Jose R. [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Biociencias. Dept. de Botanica

    2007-07-01

    The phytochemical investigation of the roots of E. almawillia is reported for the first time. Chromatographic fractionation of the methanol extract allowed the isolation of the alkaloids 3,3-diisopentenyl-N-methyl-2,4-quinoldione (1), maculine (2) and 3'-methoxygraveoline (3), (E)-N-isobutyl-3- methoxy-4,5-methylenedioxicinnamoyl amide (4), the flavones gardenine B (5) and nevadensin (6), and the sesquiterpene intermediol (7). Structure elucidation was based on the analysis of their spectrometric data (uni- and bidimensional {sup 1}H and {sup 13}C NMR, MS and IR) and comparison with literature data. Compounds 3-7 are being reported as constituents of Esenbeckia species for the first time. (author)

  1. Novel N-benzoyltyramines of Swinglea glutinosa (Rutaceae); Novas N-benzoiltiraminas de Swinglea glutinosa (Rutaceae).

    Energy Technology Data Exchange (ETDEWEB)

    Cerqueira, Cristovam do Nascimento; Santos, Djalma A.P. dos; Malaquias, Karla da Silva; Lima, Murilo Marinho de Castro; Silva, Maria Fatima das Gracas Fernandes da; Fernandes, Joao Batista; Vieira, Paulo Cezar, E-mail: dmfs@ufscar.br [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica

    2012-07-01

    Phytochemical studies of the leaves and fruits have led to the identification of the known amides (E)-N-methyl-cinnamamide, N-benzoyltyramine, N-benzoyl-O-geranyl tyramine, N-benzoyl-O-(4-acetoxyl)-geranyl tyramine, in addition to the new N-{l_brace}2-[4-(butoxyl-3-one)phenyl]ethyl{r_brace}benzamide, N-{l_brace}2-[4-(2,3-dihydroxy-2-methyl-butoxylanal)phenyl]ethyl{r_brace}benzamide, N-{l_brace}2-[4-(2,3-dihydroxy-2-methyl-butoxyloic) phenyl]ethyl{r_brace}benzamide, N-benzoyl-O-(4-acetoxyl-6,7-epoxy)-geranyltyramine, N-benzoyl-O-(4-acetoxyl-6,7-dihydroxy)-geranyltyramine and N-benzoyl-O-(6-acetoxyl-4,7-dihydroxy)-geranyltyramine. The isolated compounds clearly point to Swinglea phytochemical affinities with other Aurantioideae species. (author)

  2. Indolopyridoquinazoline alkaloids from Esenbeckia grandiflora mart. (Rutaceae); Alkaloides {beta}-indolopiridoquinazolinicos de Esenbeckia grandiflora mart. (Rutaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Januario, Ana Helena; Vieira, Paulo Cezar; Silva, Maria Fatima das Gracas Fernandes da; Fernandes, Joao Batista [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica], e-mail: anahjanuario@unifran.br; Silva, Jorge Jose de Brito; Conserva, Lucia Maria [Universidade Federal de Alagoas (UFAL), Maceio, AL (Brazil). Inst. de Quimica e Biotecnologia

    2009-07-01

    The chemical composition of two specimens of Esenbeckia grandiflora, collected in the south and northeast regions of Brazil, was investigated. In this study, three b-indolopyridoquinazoline alkaloids from the leaves (rutaecarpine, 1-hydroxyrutaecarpine) and roots (euxylophoricine D) were isolated for the first time in this genus. In addition, the triterpenes {alpha}-amyrin, {beta}-amyrin, {alpha}-amyrenonol, {beta}-amyrenonol, 3{alpha}-hydroxy-ursan-12-one, and 3{alpha}-hydroxy-12,13-epoxy-oleanane, the coumarins auraptene, umbelliferone, pimpinelin, and xanthotoxin, the furoquinoline alkaloids delbine and kokusaginine, and the phytosteroids sitosterol, stigmasterol, campesterol and 3{beta}-O-{beta}-D-glucopyranosylsitosterol were also isolated from the leaves, twigs, roots and stems of this species. Structures of these compounds were established by spectral analysis. (author)

  3. Isolation of volatile compounds of Aloe excelsa (Berger ...

    African Journals Online (AJOL)

    PROMOTING ACCESS TO AFRICAN RESEARCH ... Industrial and pharmacological applications of volatile and non-volatile compounds isolated ... Three commercially important compounds, namely: phenylacetonitrile, carvone and limonene were identified using gas ... EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT

  4. Chemical constituents of the wood from Zanthoxylum quinduense Tul. (Rutaceae)

    International Nuclear Information System (INIS)

    Patino Ladino, Oscar Javier; Cuca Suarez, Luis Enrique

    2010-01-01

    Phytochemical investigation of the wood from Zanthoxylum quinduense Tul. allowed the isolation and identification of norchelerythrine, decarine, 6-acetonyldihydrochelerythrine, syringaresinol, evofilin C, p-hydroxybenzaldehyde, vanillic acid, a mixture of b-sitosterol, stigmasterol and campesterol and a mixture of saturated and unsaturated fatty acids, and their esters derivatives. The structures of the isolated compounds were elucidated by spectroscopic techniques and comparison with literature data and the mixture of sterols and fatty acids were identified by GC/MS. The antifungal activity of the ethanolic extract, fractions and pure compounds against Fusarium oxysporum f. sp. lycopersici was determined by bioautography. Evofilin C and nochelerytrine were the only substances that present antifungal activity. (author)

  5. Prenylindole alkaloids from Raputia praetermissa (Rutaceae) and their chemosystematic significance

    Energy Technology Data Exchange (ETDEWEB)

    Rosas, Lisandra V.; Veiga, Thiago Andre M.; Fernandes, Joao B.; Vieira, Paulo C.; Silva, M. Fatima das G.F. da, E-mail: dmfs@power.ufscar.b [Universidade Federal de Sao Carlos (DQ/UFSCar), SP (Brazil). Dept. de Quimica

    2011-07-01

    The dichloromethane extract from the stems of Raputia praetermissa afforded four new compounds, 4-deoxyraputindole C (1), raputimonoindole A-B (2, 3), and hexadecanyl 2-hydroxy- 4-methoxy-cinnamate (5), besides the alkaloids 5-(4-methoxymethylfuran-2-yl)-1H-indole (raputimonoindole C), furoquinolines maculosidine, robustine, evolitrine and dictamnine. The hexane extract yielded N-methyl-4-methoxyquinoline-2(1H)-one, skimmianine, cycloartenone, sitosterol, stigmasterol and sitostenone. The anthranilate alkaloid content indicates that the genus is strongly related to those included in Cusparieae tribe, but differs from Neoraputia by the absence of prenylindole alkaloids in the late, whose species have previously been placed in Raputia. (author)

  6. NUEVA BENZOIL LACTONA Y OTROS COSTITUYENTES DE Pilocarpus alvaradoii (Rutaceae

    Directory of Open Access Journals (Sweden)

    Luis E. Cuca

    2008-04-01

    Full Text Available Del extracto etanólico de hojas de Pilocarpus alvaradoii fue aislada e identificada la nueva lactona α-Benzoil-γ-valerolactona (1, junto con los triterpenos pentacíclicos conocidos lupeol (2, epibetulina (3; y las furanocumarinas bergapteno (4, psoraleno (5, y xantotoxina (6.  El aislamiento y la purificación fueron realizados por técnicas cromatográficas convencionales.   La elucidación estructural de estos compuestos se determinó mediante técnicas espectroscópicas (IR, RMN 1H, 13C, EM.

  7. NUEVA BENZOIL LACTONA Y OTROS CONSTITUYENTES DE Pilocarpus alvaradoii (Rutaceae)

    OpenAIRE

    Cuca, Luis E.; Ávila, Mónica C.

    2007-01-01

    Del extracto etanólico de hojas de Pilocarpus alvaradoii fue aislada e identificada la nueva lactona α-Benzoil-γ-valerolactona 1, junto con los triterpenos pentacíclicos conocidos lupeol 2, y epibetulina 3; y las furanocumarinas bergapteno 4, psoraleno 5, y xantotoxina 6. El aislamiento y la purificación fueron realizados por técnicas cromatográficas convencionales. La elucidación estructural de estos compuestos se determinó mediante técnicas espectroscópicas (IR, RMN 1H, 13C, EM). From th...

  8. Nueva benzoil lactona y otros costituyentes de pilocarpus alvaradoii (rutaceae)

    OpenAIRE

    Cuca, Luis E.; Avila, Mónica C.

    2008-01-01

    Del extracto etanólico de hojas de Pilocarpus alvaradoii fue aislada e identificada la nueva lactona α-Benzoil-γ-valerolactona (1), junto con los triterpenos pentacíclicos conocidos lupeol (2), epibetulina (3); y las furanocumarinas bergapteno (4), psoraleno (5), y xantotoxina (6).  El aislamiento y la purificación fueron realizados por técnicas cromatográficas convencionales.   La elucidación estructural de estos compuestos...

  9. Phytochemical and chemosystematic studies of Euxylophora paraensis (Rutaceae)

    International Nuclear Information System (INIS)

    Isidoro, Marsele Machado; Silva, Maria Fatima das Gracas Fernandes da; Fernandes, Joao Batista; Vieira, Paulo Cezar; Arruda, Alberto C.; Silva, Sebastiao da Cruz

    2012-01-01

    Phytochemical studies of the leaves and stem have led to the identification of the known coumarins isooxypeucedanin, oxypeucedanin hydrate, xanthotoxin, isopimpinellin, 8-methoxymarmesin and marmesin, flavonoids quercetin-3-O-α-L-rhamnopyranoside, myricetin-3-O-α-L-rhamnopyranoside and hesperidin, alkaloids skimmianine and N-methylflindersine and limonoid limonin. The compounds isolated and the chemical profile of Euxylophora obtained from the literature clearly indicate its phytochemical affinities with other Rutoideae species. (author)

  10. Estudo FitoquÃmico de Pilocarpus sulcatus Skorupa (Rutaceae)

    OpenAIRE

    AntÃnio HonÃrio de Sousa

    2009-01-01

    O presente trabalho descreve o estudo fitoquÃmico de Pilocarpus sulcatus Skorupa e o levantamento acerca dos metabÃlitos secundÃrios jà isolados do tÃxon Pilocarpus. O trabalho envolveu a extraÃÃo e identificaÃÃo dos constituintes volÃteis das folhas, casca do caule, lenho do caule, cascas das raÃzes e lenho das raÃzes alÃm do isolamento e determinaÃÃo estrutural dos constituintes fixos das folhas e lenho do caule de P. sulcatus. Trata-se de uma espÃcie com descriÃÃo botÃnica recente, ainda s...

  11. Chemical constituents of the wood from Zanthoxylum quinduense Tul. (Rutaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Patino Ladino, Oscar Javier; Cuca Suarez, Luis Enrique, E-mail: ojpatinol@unal.edu.c [Universidad Nacional de Colombia, Bogota (Colombia). Facultad de Ciencias. Dept. de Quimica

    2010-07-01

    Phytochemical investigation of the wood from Zanthoxylum quinduense Tul. allowed the isolation and identification of norchelerythrine, decarine, 6-acetonyldihydrochelerythrine, syringaresinol, evofilin C, p-hydroxybenzaldehyde, vanillic acid, a mixture of b-sitosterol, stigmasterol and campesterol and a mixture of saturated and unsaturated fatty acids, and their esters derivatives. The structures of the isolated compounds were elucidated by spectroscopic techniques and comparison with literature data and the mixture of sterols and fatty acids were identified by GC/MS. The antifungal activity of the ethanolic extract, fractions and pure compounds against Fusarium oxysporum f. sp. lycopersici was determined by bioautography. Evofilin C and nochelerytrine were the only substances that present antifungal activity. (author)

  12. Taksonomic study on Micromelum Blume (Rutaceae in Indonesia

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    TAHAN UJI

    2005-04-01

    Full Text Available Taxonomic study on Micromelum Blume was conducted at Herbarium Bogoriense (BO and Kew Herbarium (K and using 84 collections number of herbarium specimen. This research used descriptive method based on morphological characters. Terminology by Stearn (1983 was used, whereas the revision method as described by Rifai (1976 and Vogel (1987 were used. Based on this study were produced two species only of Micromelum respectively which occurred in Indonesia. They were Micromelum diversifolium Miq. and M. minutum (Forst. f. Wight. & Arn. M. diversifolium had two varieties, i.e. M. diversifolium Miq. var. diversifolium and M. diversifolium Miq. var. cuneata Miq. Whereas M. minutum had three varieties, i.e. M. minutum (Forst. f. Wight. & Arn. var. minutum, M. minutum (Forst. f. Wight & Arn. var. tomentosum Tan. and M. minutum (Forst. f. Wight & Arn. var. villosum Tan. New record specimen collections of M. diversifolium from Biak Island (Papua was reported also.

  13. Apoptotic properties of Citrus sudachi Hort, ex Shirai (Rutaceae ...

    African Journals Online (AJOL)

    Methods: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and annexin V/propidium iodidle assay were used to test the antiproliferative activity and apoptosis of methanol extract of Citrus sudachi, respectively. Griess reaction and reverse transcriptase-polymerase chain reaction (RT-PCR) were carried ...

  14. Apoptotic properties of Citrus sudachi Hort, ex Shirai (Rutaceae ...

    African Journals Online (AJOL)

    ISSN: 1596-5996 (print); 1596-9827 (electronic) ... Results: Citrus sudachi exerted cytotoxicity in a time-dependent manner in cancer cells which ... NO• produced by activation of nitric oxide synthase (NOS), while it suppressed the levels .... ethanol and kept on ice for 30 min. ... reagent and incubated at room temperature for.

  15. Sustaining Quercus humboldtii and Colombobalanus excelsa on the Colombian landscape: preservation or conservation - a research perspective

    Science.gov (United States)

    Daniel C. Dey; Alejandro A. Royo; Emile S. Gardiner; Luis Mario Cardenas

    2012-01-01

    The Tropical Andes in Colombia is in the top 25 biodiversity hotspots in the world (Myers and others 2000). It has the highest level of species endemism in the world and they comprise 12 percent of the world’s species. Humans have lived in the Colombian Andes for over 12,000 years. Population estimates of native peoples at the time of Spanish contact range between 3 to...

  16. Sustaining Quercus humboltii and Colombobalanus excelsa on the Colombian landscape: preservation or conservation - a research perspective

    Science.gov (United States)

    Daniel C. Dey; Alejandro A. Royo; Emile S. Gardiner; Luis Mario. Cardenas

    2012-01-01

    The Tropical Andes in Colombia is in the top 25 biodiversity hotspots in the world (Myers and others 2000). It has the highest level of species endemism in the world and they comprise 12 percent of the world's species. Humans have lived in the Colombian Andes for over 12,000 years. Population estimates of native peoples at the time of Spanish contact range between...

  17. Phenology of brazil nut (Bertholletia excelsa Humb.& Bonpl., Lecythidaceae in south of Roraima state

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    Helio Tonini

    2011-03-01

    Full Text Available This work was carried out in order to study the phenological pattern of brasil-nut trees in natural forest located in the south of Roraima state, in order to relate the frequency of occurrence of phenophases with rainfall. For the phenological survey 20 adult trees (DBH > 50 cm were selected in a permanent sample plot of 9 ha. The phenological observations occurred fortnightly from February 2006 to February 2009, when data were collected on the presence or absence of events of flowering, fruiting, leaf flushing and leaf fall for each tree. The Index of population synchrony was used for estimating the synchrony of phenological events. The flowering of brasil-nut proved to be regular, annual, long and synchronous and was correlated with the reduction of rainfall. The fruiting was regular and synchronous, and dispersal was correlated with rainfall reduction. The phenological pattern of leaf flushing tended to vary yearly, being around the continual one in 2007 and bimodal in 2006 and 2008. It was noticed a higher proportion of trees falling leaves between August and October which characterizes a period of transition between the dry and the rainy time with sensitive reduction of rainfall.

  18. Phytochemical and chemosystematic studies of Conchocarpus marginatus and C. inopinatus (Rutaceae); Fitoquimica e quimiossistematica de Conchocarpus marginatus e C. inopinatus (Rutaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Bellete, Barbara Sayuri; Sa, Israel Civico Gil de; Mafezoli, Jair; Cerqueira, Cristovam do Nascimento; Silva, Maria Fatima das Gracas Fernandes da; Fernandes, Joao Batista; Vieira, Paulo Cezar; Zukerman-Schpector, Julio [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica; Pirani, Jose Rubens, E-mail: dmfs@ufscar.br [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Inst. de Biociencias

    2012-07-01

    Phytochemical studies of the leaves and stem have led to the identification of the known acridone alkaloids arborinine, methylarborinine, 1-hydroxy-3-methoxy-N-methyl acridone, xanthoxoline, 1,2,3,5-tetramethoxy-N-methylacridone, toddaliopsin C and the new seco acridone alkaloid inopinatin. The known quinoline alkaloids 2-phenyl-1-methyl-quinolin-4(1H)-one, 2-phenyl-1-methyl-7-methoxy-quinolin-4(1H)-one, dictamnine, and the coumarins scopoletin and marmesin were also isolated. The isolated compounds and the distribution of secondary metabolites, which are systematically important, obtained from literature, clearly confirmed that some species formerly described in the genera Angostura and Galipea in fact shall belong to the genus Conchocarpus. (author)

  19. Atividade anti-helmíntica do extrato aquoso das folhas de Zanthoxylum rhoifolium Lam. (Rutaceae Anthelmintic activity of aqueous extract of Zanthoxylum rhoifolium Lam. leaves (Rutaceae

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    Peneluc Taíse

    2009-12-01

    Full Text Available O estudo avaliou a atividade anti-helmíntica do extrato aquoso das folhas de Zanthoxylum rhoifolium em dois experimentos. O teste in vitro utilizou cultivos de fezes de caprinos tratados com diferentes concentrações do extrato: (134,5 a 335,0 mg.mL-1. No teste in vivo, utilizaram-se 20 ovelhas: G1 tratado durante quatro dias (0,63 g.kg-1 PV; G2, mesma dose, por oito dias; G3, ivermectina (200 μg.kg-1; e G4, sem tratamento. In vitro, observou-se redução de larvas de Haemonchus, Trichostrongylus e Oesophagostomum superior a 95% nas concentrações de 193,7 a 335,0 mg.mL-1. In vivo, a redução de ovos por grama de fezes foi de 51, 56 e 90%, respectivamente, para G1, G2 e G3, no oitavo dia de tratamento, enquanto para os estágios imaturos e adultos variou de 0 a 91% no G1 e 26 a 94% no G2. A eficácia da ivermectina alcançou 99% para L4 e L5 de H. contortus e 100% para as demais espécies de nematoides. Parâmetros clínicos e bioquímicos permaneceram na faixa de normalidade, e as análises histopatológicas não revelaram alterações sugestivas de toxicidade. Embora altamente efetivo in vitro, o extrato aquoso de folhas de Z. rhoifolium foi pouco eficaz in vivo na redução de nematoides gastrintestinais.The study evaluated the anthelmintic activity of aqueous extract of Zanthoxylum rhoifolium leaves in two experiments. In vitro test, cultures of goat fecal samples were treated with different concentrations of extract (134.5 to 335.0 mg.mL-1. In vivo test was composed of 20 sheep: G1: treated with 0.63 g.kg-1, during four days; G2: same dose, for eight days; G3: ivermectin (200 μg.kg-1 and G4 untreated group. In vitro results showed a reduction of Haemonchus spp, Trichostrongylus spp. and Oesophagostomum spp. larvae greater than 95% in the concentrations between 335.0 and 193.7 mg.mL-1. Faecal egg counting reduction was 51, 56 and 90% in G1, G2 and G3, respectively, while immature stages and adults ranged from 0 to 91% in G1 and from 26 to 94% in G2. Ivermectin effectiveness was 99% for L4 and L5 of H. contortus and 100% for other nematodes species. Clinical and biochemical parameters have remained in the normality and histophatologic analyses did not show alteration suggesting absence of toxicity. Although the great effectiveness of Z. rhoifolium leaves extract in vitro test, it displayed poor efficiency in vivo regarding gastrointestinal nematodes reduction.

  20. Phytochemical and chemosystematic studies of Conchocarpus marginatus and C. inopinatus (Rutaceae)

    International Nuclear Information System (INIS)

    Bellete, Barbara Sayuri; Sa, Israel Civico Gil de; Mafezoli, Jair; Cerqueira, Cristovam do Nascimento; Silva, Maria Fatima das Gracas Fernandes da; Fernandes, Joao Batista; Vieira, Paulo Cezar; Zukerman-Schpector, Julio; Pirani, Jose Rubens

    2012-01-01

    Phytochemical studies of the leaves and stem have led to the identification of the known acridone alkaloids arborinine, methylarborinine, 1-hydroxy-3-methoxy-N-methyl acridone, xanthoxoline, 1,2,3,5-tetramethoxy-N-methylacridone, toddaliopsin C and the new seco acridone alkaloid inopinatin. The known quinoline alkaloids 2-phenyl-1-methyl-quinolin-4(1H)-one, 2-phenyl-1-methyl-7-methoxy-quinolin-4(1H)-one, dictamnine, and the coumarins scopoletin and marmesin were also isolated. The isolated compounds and the distribution of secondary metabolites, which are systematically important, obtained from literature, clearly confirmed that some species formerly described in the genera Angostura and Galipea in fact shall belong to the genus Conchocarpus. (author)

  1. Phytochemical study from root barks of Zanthoxylum rigidum Humb. and Bonpl. ex Willd (Rutaceae)

    International Nuclear Information System (INIS)

    Moccelini, Sally Katiuce; Silva, Virginia Claudia da; Ndiaye, Eliane Augusto; Sousa Junior, Paulo Teixeira de; Vieira, Paulo Cezar

    2009-01-01

    Chemical investigation from root barks of Z. rigidum, resulted in the isolation of lupeol, a mixture of steroids campesterol, sitosterol, stigmasterol, sacarose, hesperidin, N-methylatanine and 6-acetonyldihydrochelerythrine. Their structures were established by spectral data analysis. No previous work has been reported on Z. rigidum species. (author)

  2. Evaluation of the tickcide, genotoxic, and mutagenic effects of the Ruta graveolens L. (Rutaceae

    Directory of Open Access Journals (Sweden)

    Alessandra Vargas de Carvalho

    2015-10-01

    Full Text Available Current analysis investigated the tickcide effects of the aqueous extract and chloroform fractions of Ruta graveolens L. (rue on engorged females of Rhipicephalus microplus, as well as their genotoxic and mutagenic effects on human leukocytes. The best tickcide activity (non-dependent dose and genotoxic / mutagenic effects (dependent-dose were observed on exposure to chloroform fractions. Results suggest that extract fractions of R. graveolens L are efficient against R. microplus, although the fraction and the tested concentrations show genotoxic and mutagenic potential for human leukocytes.

  3. Actividad larvicida de la toronja, Citrus paradisi (Rutaceae sobre dos vectores del dengue

    Directory of Open Access Journals (Sweden)

    Jorge Morales-Saldaña

    2013-04-01

    Full Text Available La actividad larvicida del aceite esencial de la toronja (Citrus paradisi fue evaluada contra los mosquitos Aedes aegypti y Aedes albopictus, los cuales representan vectores potenciales de la fiebre del dengue. La CL50 del aceite esencial de la toronja fue de 47,3 ppm y 85,1 ppm para Ae. aegypti y Ae. albopictus, respectivamente. El análisis del aceite por GC-MS, mostró químicos conocidos como insecticidas los cuales podría explicar la acción tóxica sobre las larvas de los mosquitos evaluados. Esta actividad tóxica sobre Ae. aegypti fue mayor en comparación con otros aceites cítricos, lo que sugiere mayores instigaciones en esta línea y de esta manera poder recomendarlo como regulador de mosquitos a las diferentes campañas contra el Ae. aeygpti. En la literatura revisada no encontramos ninguna investigación donde haya sido evaluado aceites esenciales de cítricos contra larvas del mosquito Ae. albopictus.

  4. Characterization of Microsatellites for the Endangered Ruta oreojasme (Rutaceae and Cross-Amplification in Related Species

    Directory of Open Access Journals (Sweden)

    Marilena Meloni

    2013-04-01

    Full Text Available Premise of the study: Ruta oreojasme is an endangered species endemic to Gran Canaria (Canary Islands, Spain, where it occurs in small populations with disjunct distribution. Nothing is known about the genetic structure of these populations. Methods and Results: Using a microsatellite-enriched library method, 10 microsatellite markers have been developed from R. oreojasme, all of which showed polymorphism. The transferability of the 10 markers was tested in two other Canarian endemic species, R. microcarpa and R. pinnata, as well as in the widespread species R. montana. Conclusions: Our results demonstrate the value of these newly developed microsatellite markers to investigate the genetic structure in R. oreojasme and show their potential applicability for population genetic studies in other Ruta species.

  5. Low antiplasmodial activity of alkaloids and amides from the stem bark of Zanthoxylum rubescens (Rutaceae

    Directory of Open Access Journals (Sweden)

    Penali L.

    2007-06-01

    Full Text Available The stem bark of Zanthoxylum rubescens (syn. Fagara rubescens is used for treating fevers associated with malaria in the Ivory Coast. Three alkaloids: N-nornitidine, 7,9-dimethoxy-2,3- methylenedioxybenzophenanthridine, and bis[6-(5,6- dihydrochelerythrinyl] ether; and two amides: zanthomamide and lemairamide, were isolated from the stem bark of this plant. These compounds were screened in vitro against the chloroquine-sensitive 3D7 strain and the chloroquine-resistant FCM29 strain of P. falciparum. N-nornitidine was found to be inactive. 7,9- dimethoxy-2,3-methylenedioxybenzophenanthridine, lemairamide and zanthomamide showed weak activity with average IC50 values ranging from 45.6 μM to 149.9 μM. Bis[6-(5,6- dihydrochelerythrinyl] ether was the most active of the tested compounds with mean IC50s of 14.9 ± 1.4 μM in FCM29 strain and 15.3 ± 3.4 μM in 3D7 strain (~ 58 to ~ 1130 times less active than chloroquine respectively. The anti-Plasmodium activities of the tested alkaloids of Z. rubescens were low; and do not encourage the use of this plant as antimalarial.

  6. Fitoquímica e quimiossistemática de Conchocarpus marginatus e C. inopinatus (Rutaceae

    Directory of Open Access Journals (Sweden)

    Barbara Sayuri Bellete

    2012-01-01

    Full Text Available Phytochemical studies of the leaves and stem have led to the identification of the known acridone alkaloids arborinine, methyl-arborinine, 1-hydroxy-3-methoxy-N-methyl acridone, xanthoxoline, 1,2,3,5-tetramethoxy-N-methylacridone, toddaliopsin C and the new seco acridone alkaloid inopinatin. The known quinoline alkaloids 2-phenyl-1-methyl-quinolin-4(1H-one, 2-phenyl-1-methyl-7-methoxy-quinolin-4(1H-one, dictamnine, and the coumarins scopoletin and marmesin were also isolated. The isolated compounds and the distribution of secondary metabolites, which are systematically important, obtained from literature, clearly confirmed that some species formerly described in the genera Angostura and Galipea in fact shall belong to the genus Conchocarpus.

  7. Fire effects on the population structure of Zanthoxylum rhoifolium Lam (Rutaceae in a Brazilian savanna

    Directory of Open Access Journals (Sweden)

    IA. Silva

    Full Text Available Since cerrado fires may impede the growth of seedlings into trees, they may shape the population of woody species. In this study, we assessed the effects of a severe fire on the population structure and spatial distribution of Zanthoxylum rhoifolium, a widespread cerrado tree. We were interested to know the importance of the resprouting and sexual reproduction in regenerating the population. The study area had been for about six years protected from fire, before a severe fire at the end of the dry season in 2006. We sampled and measured all individuals of Z. rhoifolium found in 80 plots of 25 m². We found 149 individuals before the fire and 112 after the fire, of which 77 were resprouts from burned seedlings and saplings. We did not find significant differences between the population structure before and after the fire. The spatial distribution of the population remained clumped after the fire. Thus, the Z. rhoifolium population was very resilient to a severe fire. We did not find any new seedlings. As a consequence, resprouting seems to be more important than sexual reproduction in promptly regenerating the Z. rhoifolium populations.

  8. Association between aflatoxin and aflatoxigenic fungi in Brazil nut (Bertholletia excelsa H.B.K. Associação de aflatoxinas e fungos aflatoxigênicos em castanha-do-Brasil (Bertholletia excelsa H.B.K.

    Directory of Open Access Journals (Sweden)

    Ariane Mendonça Pacheco

    2010-06-01

    Full Text Available Brazil nut has a high nutritional content and is a very important trade commodity to some Latin American countries. In order to evaluate its safety, 120 samples from different stages of the productive chain were analyzed in terms of: moisture content (mc, aflatoxigenic fungi and aflatoxin (LOQ = 1.95 μg.kg-1 total aflatoxin using TLC. Among all samples, 4 (6.7% from the receiving area, and 5 (16.7%, from retail presented aflatoxins above the LOQ, but the amount of aflatoxins was below the LOQ after the samples were dried in the plant. The positive samples were above the limit of total aflatoxin permitted by the European Union (4.0 μg.kg-1 and Brazil (30 μg.kg-1. The mc mean was 22.43% in the receiving area, which is higher than that in the other stages samples. All the A. flavus strains were aflatoxigenic, and there was statistic association between the presence of aflatoxin and flavus strains. The aflatoxigenic fungi strains associated to the aflatoxins levels in the samples show that an effective control is necessary for the food safety in the Brazil nut production chain.A castanha-do-Brasil tem um alto teor nutricional e é um importante produto comercial para alguns países da América Latina. Com objetivo de avaliar a segurança, 120 amostras de diferentes etapas da cadeia produtiva, foram analisadas quanto a: teor de umidade, fungos aflatoxigênicos e aflatoxinas (LOQ = 1,95 μg.kg-1 aflatoxinas totais por CCD. Das amostras, 4 (6,7% da área de recepção, e 5 (16,7% do varejo apresentaram aflatoxinas acima do LOQ. Nas amostras após secagem na usina não foram detectadas aflatoxinas(< LOQ. As amostras positivas estavam acima dos limites aceitos pela União Européia (4,0 μg.kg-1 e Brasil (30 μg.kg-1. A média do teor de umidade foi de 22,43% na etapa de recepção, maior do que as outras etapas estudadas. Todas as cepas de A. flavus eram aflatoxigênicas e houve associação estatística entre a presença de aflatoxinas e a presença da cepa de flavus. As cepas aflatoxigênicas associadas às aflatoxinas das amostras demonstram que é neccessario um controle efetivo da segurança alimentar na cadeia produtiva da castanha-do-Brasil

  9. Mezclas binarias y ternarias del aceite y grasa hidrogenada de la castaña de Brasil (Bertholletia excelsa

    Directory of Open Access Journals (Sweden)

    Polakiewicz, Bronislaw

    2000-12-01

    Full Text Available Crude Brazilian nut oil was hydrogenated at 175ºC, 3 atm., 60 minutes (GH1, and 150ºC, 1 atm., 30 minutes (GH2, and with this products and the crude oil were prepared ternary blends. Fatty acids demonstrated the increase of stearic acid from 11.6% of crude oil to 56.7% in GH1 and 16.3% in GH2. In the blends, were determined the yield point, solid fat content, melting point and viscosity, for this blends were applied a special model of multiple regression. Results showed that the estimated properties were not dependent on the three components interactions. In general, the significant interactions, blends crude oil/GH1 and GH1/GH2, showed an antagonic, effect to consistency and solid fat content, typical of eutectic interactions of fats. The interactions were not significative for melting point and viscosity at 60ºC, attesting a perfect mixing model.El aceite de castaña de Brasil crudo se sometió a dos hidrogenaciones, a 175ºC, 3 atm, 60 minutos (GH1, y a 150ºC, 1 atm., 30 minutos (GH2, y con los productos de éstas, y el aceite original, se prepararon mezclas binarias y ternarias. Se incrementó la concentración del ácido esteárico de 11.6% del aceite original a 56.7% en GH1 y a 16.3% en GH2. Se realizaron los análisis de consistencia, contenido de grasa sólida, punto de fusión y viscosidad, y sobre estos parámetros se aplicó un modelo de regresión múltiple. Los resultados indicaron que las propiedades estimadas no dependían de las interacciones de los tres componentes. Las interacciones fueron significativas para las mezclas de aceite bruto/GH1 y GH1/GH2, demostrando efecto antagónico para la consistencia y contenido de grasa sólida. Las interacciones no fueron significativas para el punto de fusión y viscosidad a 60ºC, demostrando un modelo perfecto de mezcla.

  10. Hidrogenación e interesterificación del aceite de castaña de Brasil (Bertholletia excelsa

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    Polakiewicz, Bronislaw

    2001-08-01

    Full Text Available Brazil nut oil (ACB was hydrogenated in a 1L Parr reactor, with Ni as catalyst, at the following process conditions: 175ºC, 3 atm, 60 min (GH1, 150ºC, 1 atm, 30 min (GH2 and 125ºC, 1 atm, 30 min (GH3. Different proportions of blends with ACB and GH1 and GH2 were prepared. These mixtures were interesterified at laboratory scale (0.75% of sodium metoxide, 60 min, 60-65ºC. Linoleic selectivity (Sl was 3.87 (GH1, 17.46 (GH2 and 17.46 (GH3. Linolenic selectivity (Sln was 2.3 for every reaction. It was observed different results for starting and interesterificated blends for the physical properties, for these parameters and for the interesterified fats, were applied a multiple regression. Results showed that consistency and solid fat content (SFC were dependent on the hydrogenated fats. Significant interactions were, in general, for the interesterified blends of ACB/GH1 and GH1/GH2, only for the consistency and not for the other properties.El aceite de castaña de Brasil (ACB fue hidrogenado, en un reactor Parr de 1 L, catalizador a base de Ni, y bajo las siguientes condiciones de proceso: 175ºC, 3 atm, 60 min (GH1, 150ºC, 1 atm, 30 min (GH2 y 125ºC, 1 atm, 30 min (GH3. Con las grasas resultantes se prepararon mezclas en diferentes proporciones de ACB con GH1 y GH2. Estas fueron interesterificadas a escala de laboratorio con 0.75% de metóxido de sodio, 60 min, 60-65ºC. En la hidrogenación la selectividad linoleica (Sl fue 3.87 (GH1, 17.46 (GH2 y 8.45 (GH3 y la selectividad linolénica (Sln fue 2.3 para las tres reacciones. A los parámetros de las propiedades físicas de los productos interesterificados, se aplicó un modelo de regresión múltiple. Los resultados mostraron que la consistencia y el contenido en grasa sólida dependían de la grasa hidrogenada, e indicaron que las interacciones fueron en general, significativas para las mezclas interesterificadas de ACB/GH1 y GH1/GH2 en cuanto a la consistencia, pero no en las otras propiedades físicas.

  11. Optimized extraction of polyphenolic antioxidant compounds from Brazil nut (Bertholletia excelsa) cake and evaluation of the polyphenol profile by HPLC.

    Science.gov (United States)

    Gomes, Suellen; Torres, Alexandre G

    2016-06-01

    The solid residue (cake) of pressed Brazil nut oil has high energy value and contains high levels of nutrients and bioactive compounds, such as polyphenols. However, little is known about these components in this by-product. Extraction is the first step in investigating the phenolic compounds in Brazil nut cake because extraction conditions might impact the yields of phenolic compounds and antioxidant capacity. The aim of this study was to select the best phenolic compound extraction conditions for Brazil nut cake by using factorial experimental design and to characterize the phenolic compounds in the extract. The optimal extraction of antioxidant phenolic compounds from Brazil nut cake was achieved under the following conditions: ethanol-water (40:60; v/v); 2.5 min homogenization; and 1 h extraction at 60 °C. The phenolic compound profile of the Brazil nut cake extract using the optimized extraction was determined by high-performance liquid chromatography with photodiode array detection. Six phenolic acids (gallic acid, protocatechuic acid, 2,4-dihydroxybenzoic acid, p-hydroxybenzoic acid, p-coumaric acid and sinapic acid) and one flavonoid ((+)-catechin) were identified, and the contents of the phenolic compounds varied from 70.0 to 421 mg kg(-1) . Knowledge of the potential bioactivity of Brazil nut cake identified in the present study might promote its use in the food industry. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  12. Atributos do Solo Relacionados com a Produção da Castanheira-do-Brasil (Bertholletia excelsa

    Directory of Open Access Journals (Sweden)

    Mirian Gomes Costa

    2017-05-01

    Full Text Available RESUMO Neste estudo foram analisados os atributos do solo relacionados com variações na produção de frutos da castanheira bem como o estado nutricional da espécie. As árvores foram selecionadas em parcela permanente (Caracaraí, RR e divididas em quatro classes de produção: alta, média, baixa e muito baixa. Em novembro de 2009, amostras de solo e folhas foram coletadas para se avaliar a fertilidade do solo, o número de esporos de fungos micorrízicos arbusculares e o estado nutricional das árvores. Árvores com maior produção de frutos ocorreram associadas a maior teor de Al trocável na camada de 0-20 cm do solo, bem como a menor valor de pH e saturação por bases. A variável discriminante resultante deste estudo não foi considerada como responsável por interferir no potencial produtivo da castanheira-do-brasil, mas serviu como indicativo de que árvores mais produtivas contribuem na remoção de bases trocáveis, favorecendo a acidificação do solo.

  13. Phytochemical study from root barks of Zanthoxylum rigidum Humb. and Bonpl. ex Willd (Rutaceae); Estudo fitoquimico das cascas das raizes de Zanthoxylum rigidum Humb. and Bonpl. ex Willd (Rutaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Moccelini, Sally Katiuce; Silva, Virginia Claudia da; Ndiaye, Eliane Augusto; Sousa Junior, Paulo Teixeira de [Universidade Federal de Mato Grosso (UFMT), Cuiaba, MT(brazil). Dept. de Quimica; Vieira, Paulo Cezar [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Quimica]. E-mail: teixeira@ufmt.br

    2009-07-01

    Chemical investigation from root barks of Z. rigidum, resulted in the isolation of lupeol, a mixture of steroids campesterol, sitosterol, stigmasterol, sacarose, hesperidin, N-methylatanine and 6-acetonyldihydrochelerythrine. Their structures were established by spectral data analysis. No previous work has been reported on Z. rigidum species. (author)

  14. Quinoline alkaloids and friedelane-type triterpenes isolated from leaves and wood of Esenbeckia alata Kunt (Rutaceae)

    International Nuclear Information System (INIS)

    Cuca-Suarez, Luis Enrique; Barrera, Ericsson David Coy; Alvarez Caballero, Juan Manuel

    2011-01-01

    This work describes the phytochemical exploration of the ethanol extract from leaves and wood of Esenbeckia alata, leading to the isolation and identification of quinoline alkaloids 4-methoxy-3-(3'-methyl-but-2'-enyl)-N-methyl-quinolin-2(1 H)-one, N-methylflindersine, dictamine, kokusaginine, G-fagarine, flindersiamine, as well as the fridelane-type triterpenes, frideline, fridelanol and its acetate derivative. Identification of these compounds was based on full analyses of spectroscopic data ( 1 H, 13 C, 1 D, 2 D, IR, MS) and comparison with data reported in literature. Compound 4-methoxy-3-(3'-methyl-but-2'-enyl)-N-methyl-quinolin-2(1 H)-one is reported for the first time for the genus Esenbeckia. (author)

  15. Quinoline alkaloids and friedelane-type triterpenes isolated from leaves and wood of Esenbeckia alata Kunt (Rutaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Cuca-Suarez, Luis Enrique; Barrera, Ericsson David Coy [Universidad Nacional de Colombia, Bogota (Colombia). Dept. de Quimica; Alvarez Caballero, Juan Manuel [Universidad del Magdalena, Santa Marta DTCH (Colombia). Facultad de Ciencias Basicas

    2011-07-01

    This work describes the phytochemical exploration of the ethanol extract from leaves and wood of Esenbeckia alata, leading to the isolation and identification of quinoline alkaloids 4-methoxy-3-(3'-methyl-but-2'-enyl)-N-methyl-quinolin-2(1 H)-one, N-methylflindersine, dictamine, kokusaginine, G-fagarine, flindersiamine, as well as the fridelane-type triterpenes, frideline, fridelanol and its acetate derivative. Identification of these compounds was based on full analyses of spectroscopic data ({sup 1}H, {sup 13}C, {sup 1}D, {sup 2}D, IR, MS) and comparison with data reported in literature. Compound 4-methoxy-3-(3'-methyl-but-2'-enyl)-N-methyl-quinolin-2(1 H)-one is reported for the first time for the genus Esenbeckia. (author)

  16. Pharmacognostic study and development of quality control parameters for fruit, bark and leaf of Zanthoxylum armatum (Rutaceae

    Directory of Open Access Journals (Sweden)

    Fiaz Alam

    2015-01-01

    Conclusion: The data generated from this study would be of help in the authentication of various parts of Z. armatum, an important constituent of various herbal drug formulations. The qualitative and quantitative microscopic features would prove useful for laying down pharmacopoeial standards. Morphology as well as various pharmacognostic aspects of different parts of the plant were studied and have been described here along with phytochemical, physicochemical studies, which will help in authentication and quality control.

  17. Effects of the essential oil obtained from Pilocarpus spicatus Saint-Hilaire (Rutaceae on the development of Rhodnius prolixus nymphae

    Directory of Open Access Journals (Sweden)

    Cícero B. Mello

    Full Text Available Bioassays against fifth-instar nymphae of Rhodnius prolixus were conducted with essential oil of Pilocarpus spicatus extracted by hydrodistillation. The main results may be summarized as follows: (i high levels of toxicity and paralysis together with discrete moulting inhibition were caused by topical application of either 0.5 µL or 1.0 µL per insect of the crude essential oil; (ii partial fagoinhibition, high moulting inhibition, prolonged intermoulting period and high number of paralyzed insects, but no toxicity were observed after oral treatment using either 5 µL or 10 µL of Pilocarpus spilcatus essential oil per mL of ingested blood meal. The importance of these results in relation to the relevant biological events in R. prolixus is herein discussed.

  18. Biting Deterrence, Repellency, and Larvicidal Activity of Ruta chalepensis (Sapindales: Rutaceae) Essential Oil and Its Major Individual Constituents Against Mosquitoes

    Science.gov (United States)

    2013-01-01

    anthraquinones, tannins , ßavonoids, cyanogenic glycosides, cardiac glycosides, triterpenes, saponins, sterols, furanocou- marins, and essential oils...and stored in sealed amber vial at 4C. Gas Chromatography–Mass Spectrometry Analysis Conditions. The gas chromatographyÐmass spectrom- etry(GCÐMS...Mass spectra were recorded at 70 with mass range of m/z 35Ð450. GCAnalyses.TheGC analysis was carried out using an Agilent 6890N GC system (SEM Ltd

  19. Estudo farmacognóstico de folhas e raízes da Spiranthera odoratissima A. St.-Hil. (Rutaceae)

    OpenAIRE

    Matos,L.G.; Fiuza,T.S.; Tresvenzol,L.M.F.; Rezende,M.H.; Bara,M.T.F.; Silveira,E.N.; Costa,E.A.; Paula,J.R

    2014-01-01

    A Spiranthera odoratissima A. St.-Hil. (manacá) é um arbusto cujas raízes são utilizadas popularmente contra dores musculares, de estômago e de cabeça, disfunções hepáticas, reumatismo, e como estimulante do apetite. As folhas são empregadas como depurativo do sangue, nas afecções renais e hepáticas. O objetivo deste trabalho foi realizar o estudo morfo-anatômico de S. odoratissima, bem como a triagem fitoquímica, a determinação dos teores de água, cinzas totais e insolúveis em ácido. O mater...

  20. Biological activity of Ruta chalepensis (Rutaceae and Sechium pittieri (Cucurbitaceae extracts on Hypsipyla grandella (Lepidoptera: Pyralidae larvae

    Directory of Open Access Journals (Sweden)

    Fernando Mancebo

    2001-06-01

    Full Text Available Biological activity of a plant extract (common rue, Ruta chalepensis and a semi purifíed fraction (from "tacaco cimarrón", Sechium pittieri on mahogany shootborer larvas (Hypsipyla grandella was studied. A randomized complete block design, with four replications, was used. H. grandella third instar larvas were exposed for 24 h to Cedrela odorata leaf discs dipped in several treatment dissolutions of each extract (0.1, 0.32, 1.0, 3.20, and 10%; afterwards, each larva was transferred to a flask containing an artificial diet and was allowed to complete its development. Variables measured included food consumption (foliar area eaten in 24 h, mortality, and developmental effects (developmental time for each larval instar and the pupa, and pupal weight. The common rue extract showed a clear antifeedant activity at a concentration as low as 0.32%, whereas the "tacaco cimarrón" fraction caused toxicity, especially at the two highest concentrations (3.20 and 10%.Se estudió la actividad biológica de un extracto de follaje de ruda (Ruta chalepensis y de una fracción semipurificada de "tacaco cimarrón" (Sechium pittieri sobre las larvas del gusano barrenador de las meliáceas (Hypsipyla grandella. Se utilizó un diseño de bloques completos al azar, con cuatro repeticiones. Durante 24 h se expusieron larvas de tercer estadio de H. grandella a discos de follaje de Cedrela odorata impregnados con cada tratamiento. Estos consistieron en disoluciones de cada extracto (0.1, 0.32, 1.0, 3.20 y 10%; posteriormente cada larva se transfirió a un frasco que contenía dieta artificial, donde se le permitió completar su desarrollo. Las variables de respuesta fueron el consumo de alimento (área foliar comida en 24 h, la mortalidad y efectos sobre el desarrollo (tiempo de desarrollo de cada estadio larval y de la pupa, y el peso de la pupa. El extracto de ruda causó fagodisuasión a una concentración de apenas 0.32%, mientras que la fracción de "tacaco cimarrón" provocó toxicidad especialmente a las dos mayores concentraciones (3.20 y 10%.

  1. Zanthoxylum gilletii (Rutaceae) - a mainly Guineo-Congolian forest tree with a newly discovered disjunction to western Ethiopia

    DEFF Research Database (Denmark)

    Friis, Ib; Vollesen, Kaj

    2010-01-01

    Regnskovstræet Zanthoxylum gilletii, der hovedsageligt kendes fra lavlansregnskov i Congo-bækkenet og Vestafrika, er fundet i riverin skov i det vestlige Etiopien. Den disjunkte udbredelse bekræfter den forbindelse, der tidligere er konstateret mellen det vestlige Etiopien og regnskovs- og savann...

  2. Estudo farmacognóstico de folhas e raízes da Spiranthera odoratissima A. St.-Hil. (Rutaceae

    Directory of Open Access Journals (Sweden)

    L.G. Matos

    2014-09-01

    Full Text Available A Spiranthera odoratissima A. St.-Hil. (manacá é um arbusto cujas raízes são utilizadas popularmente contra dores musculares, de estômago e de cabeça, disfunções hepáticas, reumatismo, e como estimulante do apetite. As folhas são empregadas como depurativo do sangue, nas afecções renais e hepáticas. O objetivo deste trabalho foi realizar o estudo morfo-anatômico de S. odoratissima, bem como a triagem fitoquímica, a determinação dos teores de água, cinzas totais e insolúveis em ácido. O material vegetal foi coletado em Leopoldo de Bulhões/ Goiás e os estudos morfo-anatômicos e a triagem fitoquímica foram realizados utilizando as técnicas convencionais. Verificou-se que a folha é hipoestomática, com estômatos anomocíticos e a epiderme apresenta papilas na face abaxial. O mesofilo é dorsiventral e a nervura principal possui drusas de oxalato de cálcio e cavidades secretoras. O pecíolo apresenta sistema vascular em forma circular. Na raiz, em crescimento primário, observou-se cilindro vascular com organização diarca. Na prospecção fitoquímica detectaram-se fenóis, taninos, cumarinas, flavonoides, triterpernos/esteróis, antraquinonas e antocianinas nas folhas e alcaloides, cumarinas, amido e saponinas nas raízes. Esses resultados são parâmetros importantes para o controle de qualidade dessa matéria-prima vegetal.

  3. Gastric Ulcers in Middle-Aged Rats: The Healing Effect of Essential Oil from Citrus aurantium L. (Rutaceae

    Directory of Open Access Journals (Sweden)

    C. M. Polo

    2012-01-01

    Full Text Available The elderly population has experienced increased life expectancy as well as the increased incidence of gastric ulcers. The peels of fruits from Citrus aurantium L., popularly known in Brazil as orange bitter, are commonly used asatea form for the treatment of gastrointestinal tract disorders, such as ulcer and gastritis. We evaluated the healing effects of essential oil from the peels of Citrus aurantium fruits (OEC on gastric ulcers in middle-aged rats. We examined the effects of a 14-day chronic OEC treatment on gastric mucosa in middle-aged male Wistar rats that were given acetic-acid-induced gastric lesions by morphometric and immunohistological analyses. Oral OEC treatment significantly reduced the lesion area (76% within the gastric mucosa and significantly increased (P<.05 the height of regenerated mucosa (59% when compared to the negative control group. Immunohistochemical analysis of the molecular markers such as COX-2, HSP-70, VEGF, and PCNA in the gastric mucosa confirmed that OEC treatment induced healing effects by increasing the number of new blood vessels and by augmenting gastric mucus in the mucosa glands. These results suggest that the oil from Citrus aurantium effectively heals gastric ulcers in middle-aged animals; however, safe use of OEC demands special care and precautions.

  4. Chemical composition and antimicrobial activity of the essential oil of the leaves of Feronia elephantum (Rutaceae) from north west Karnataka.

    Science.gov (United States)

    Joshi, Rajesh K; Badakar, Vijayalaxmi M; Kholkute, Sanjiva D; Khatib, Nayeem

    2011-01-01

    The essential oil of the leaves of Feronia elephantum Corr. was analyzed by gas chromatography and gas chromatography/mass spectrometry. The main constituents were beta-pinene (28.4%), Z-anethole (22.1%), methyl chavicol (12.0%) and E-anethole (8.1%), among thirty-three identified compounds, which represented 92.6% of the total oil. The antimicrobial activity was tested against five Gram-positive and eight Gram-negative bacteria, and four fungi. The oil was active against Micrococcus luteus (Gram-positive bacterium), Proteus mirabilis (Gram-negative bacterium), Penicillium chrysogenum and Aspergillus niger (fungi) with MIC values of 0.31 +/- 0.06, 0.52 +/- 0.10, 0.20 +/- 0.50 and 0.26 +/- 0.52 mg/mL, respectively.

  5. Behavioral responses of male Diaphorina citri (Hemiptera: Liviidae) to mating communication signals from vibration traps in citrus (Sapindales: Rutaceae) trees

    Science.gov (United States)

    The Asian citrus psyllid, Diaphorina citri Kuwayama, vectors the bacterium causing citrus greening disease, which has devastated citrus production worldwide wherever it has been introduced. To help monitor and target D citri populations in commercial groves, thereby facilitating more effective manag...

  6. Post-fire phenological behavior and breeding biology of the Spiranthera odoratissima A. St.-Hil. (Rutaceae

    Directory of Open Access Journals (Sweden)

    Mirley Luciene dos Santos

    2008-03-01

    Full Text Available The phenological behavior of species in response to cerrado stricken by fire is little known. A phenological and reproductive biological study of Spiranthera odoratissima was carried out in an anthropized area of cerrado sensu stricto affected by fire in Goiânia County, Goiás, Brazil. Phenolocial observations indicated that the individuals bloomed in synchrony three months after the fire. The phenological rhythms were associated with the seasonal pluviometric effects, a characteristic pattern of shrublet species of the cerrado. Theflowers are white with a sweet odor and are grouped in panicle inflorescences. The blossoming occurs during dusk and begins at around 16h. It offers pollen and nectar to its visitors. The species produces 32.8μl (± 3.4 of nectar with an average concentration of 16.4% (± 0.43 in equivalents of sucrose. The pollination system (phalenophily was proposed based on an analysis of the fl ower’s characteristics. Observed visitors were bees (Apis mellifera Linnaeus, flies, wasps, ants and beetles, but due to the inflorescence behavior, they were considered to be merely resource thieves. It was observed that Trigona spinipes Fabr. can act as a secondary pollinator during the pilling of pollen. The results of manual pollination and the incompatibility index (ISI indicate that the species is xenogamous and self-compatible.

  7. Low genetic diversity and intrapopulation spatial genetic structure of the Atlantic Forest tree, Esenbeckia leiocarpa Engl. (Rutaceae

    Directory of Open Access Journals (Sweden)

    G. Forti

    2014-12-01

    Full Text Available Studies on population genetics are the key to designing effective in situ management plans for tree species, in particular, those subjected to pressure from anthropogenic processes, such as forest fragmentation and logging. To investigate genetic diversity, inbreeding and intrapopulation spatial genetic structure (SGS in a fragmented population of the insect-pollinated tropical tree, Esenbeckia leiocarpa, we developed specific microsatellite markers for this species and mapped and sampled 100 individuals in a forest plot. Two issues were addressed in particular: (i the level of genetic diversity, inbreeding and effective population size, (ii whether intrapopulation spatial genetic structure exists. Among the 14 loci developed, we only used the three that presented polymorphism to estimate the genetic parameters. Genetic diversity was low, whereby the average number of alleles per locus (A was 3.3 and observed (H0 and expected heterozygosities (He were 0.336 and 0.298, respectively. The average fixation index was significantly higher than zero (F = 0.112, suggesting inbreeding. Significant SGS was found up to 7 m and between 31 to 38 m, indicating that trees growing within these distances may be related. Estimates of the effective population size indicated that the 100 sampled trees correspond to 14 individuals that are neither related nor inbred. Our results suggest that the microsatellite markers developed in this study are suitable for studies on geneticdiversity and structure, mating systems, gene flow and SGS in this species.

  8. Stereo and scanning electron microscopy of in-shell Brazil nut (Bertholletia excelsa H.B.K.): part two-surface sound nut fungi spoilage susceptibility.

    Science.gov (United States)

    Scussel, Vildes M; Manfio, Daniel; Savi, Geovana D; Moecke, Elisa H S

    2014-11-01

    This work reports the in-shell Brazil nut spoilage susceptible morpho-histological characteristics and fungi infection (shell, edible part, and brown skin) through stereo and scanning electron microscopies (SEM). The following characteristics related to shell (a) morphology-that allow fungi and insects' entrance to inner nut, and (b) histology-that allow humidity absorption, improving environment conditions for living organisms development, were identified. (a.1) locule in testae-the nut navel, which is a cavity formed during nut detaching from pods (located at 1.0 to 2.0/4th of the shell B&C nut faces linkage). It allows the nut brown skin (between shell and edible part) first contact to the external environment, through the (a.2) nut channel-the locule prolongation path, which has the water/nutrients cambium function for their transport and distribution to the inner seed (while still on the tree/pod). Both, locule followed by the channel, are the main natural entrance of living organisms (fungi and insects), including moisture to the inner seed structures. In addition, the (a.3) nut shell surface-which has a crinkled and uneven surface morphology-allows water absorption, thus adding to the deterioration processes too. The main shell histological characteristic, which also allows water absorption (thus improving environment conditions for fungi proliferation), is the (b.1) cell wall porosity-the multilayered wall and porous rich cells that compose the shell faces double tissue layers and the (b.2) soft tissue-the mix of tissues 2 faces corner/linkage. This work also shows in details the SEM nut spoilage susceptible features highly fungi infected with hyphae and reproductive structures distribution. © 2014 Institute of Food Technologists®

  9. Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae in transgenic bean plants (Phaseolus vulgaris L., Fabaceae

    Directory of Open Access Journals (Sweden)

    Aragão F.J.L.

    1999-01-01

    Full Text Available Bean (Phaseolus vulgaris, an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methionine-rich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R2 to R5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23% over the values found in untransformed plants.

  10. In Vitro Evaluation of Essential Oils Derived from Piper nigrum (Piperaceae and Citrus limonum (Rutaceae against the Tick Rhipicephalus (Boophilus microplus (Acari: Ixodidae

    Directory of Open Access Journals (Sweden)

    Rafaelle Vinturelle

    2017-01-01

    Full Text Available The present research aimed to study the chemical composition and acaricidal activity of Citrus limonum and Piper nigrum essential oils against the cattle tick Rhipicephalus microplus. GC-MS analysis of C. limonum essential oil showed limonene (50.3%, β-pinene (14.4%, and γ-terpinene (11.7% as the major components; P. nigrum oil was mainly composed of β-caryophyllene (26.2%, σ-ocymene (5.8%, and α-pinene (5.5%. Acaricide activity was evaluated at concentrations of 2.5, 5.0, and 10.0% (v/v of each plant oil, as well as 1 : 1 combination of both oils (5% : 5%, 2.5% : 2.5%, and 1.25% : 1.25% each, by immersing engorged R. microplus females for one minute. The LC90 of oils from C. limonum, P. nigrum, and the combination were 4.9%, 14.8%, and 5.1%, respectively. C. limonum essential oil caused 100% mortality of engorged females at the highest concentration (10%. P. nigrum essential oil inhibited egg-laying by up to 96% in a concentration-dependent manner, suggesting it reduces tick fecundity. When combined, the oils presented toxicity as to C. limonum oil alone, but with stronger inhibition of oviposition (5% : 5%, indicating a possible additive effect against R. microplus. The present data provide support for further investigation of novel natural products to control bovine tick infestations.

  11. Low-cost and eco-friendly green synthesis of silver nanoparticles using Feronia elephantum (Rutaceae) against Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti (Diptera: Culicidae).

    Science.gov (United States)

    Veerakumar, Kaliyan; Govindarajan, Marimuthu; Rajeswary, Mohan; Muthukumaran, Udaiyan

    2014-05-01

    Mosquitoes transmit serious human diseases, causing millions of deaths every year. The use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of synthesized natural products for vector control have been a priority in this area. In the present study, the larvicidal activity of silver nanoparticles (AgNPs) synthesized using Feronia elephantum plant leaf extract against late third-instar larvae of Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus was determined. The range of concentrations of synthesized AgNPs (5, 10, 15, 20, and 25 μg mL(-1)) and aqueous leaf extract (25, 50, 75, 100, and 125 μg mL(-1)) were tested against the larvae of A. stephensi, A. aegypti, and C. quinquefasciatus. Larvae were exposed to varying concentrations of aqueous crude extract and synthesized AgNPs for 24 h. Considerable mortality was evident after the treatment of F. elephantum for all three important vector mosquitoes. The synthesized AgNPs from F. elephantum were highly toxic than crude leaf aqueous extract to three important vector mosquito species. The results were recorded from UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy analysis (EDX). Synthesized AgNPs against the vector mosquitoes A. stephensi, A. aegypti, and C. quinquefasciatus had the following LC50 and LC90 values: A. stephensi had LC50 and LC90 values of 11.56 and 20.56 μg mL(-1); A. aegypti had LC50 and LC90 values of 13.13 and 23.12 μg mL(-1); and C. quinquefasciatus had LC50 and LC90 values of 14.19 and 24.30 μg mL(-1). No mortality was observed in the control. These results suggest that the green synthesis of silver nanoparticles using F. elephantum has the potential to be used as an ideal eco-friendly approach for the control of A. stephensi, A. aegypti, and C. quinquefasciatus. This is the first report on the mosquito larvicidal activity of the plant extracts and synthesized nanoparticles.

  12. Storage behavior and changes in concentrations of abscisic acid and gibberellins during dormancy break and germination in seeds of Phellodendron amurense var. wilsonii (Rutaceae).

    Science.gov (United States)

    Chen, Shun-Ying; Chien, Ching-Te; Baskin, Jerry M; Baskin, Carol C

    2010-02-01

    The medicinal Asian plant genus Phellodendron is known to contain several very important compounds that have biological action. The main purpose of this study was to determine whether seeds of Phellodendron amurense var. wilsonii can be stored and to characterize their dormancy. Seeds of this taxon stored at -20 and -80 degrees C and in liquid nitrogen retained their high germinability, indicating that they have orthodox storage behavior. Intact seeds from freshly collected fruits were dormant and required 12 weeks of cold stratification at 4 degrees C for complete germination. Scarifying the seed coat was partially effective in breaking seed dormancy. Exogenous gibberellins (GA(3), GA(4) and GA(4+7)) promoted germination of scarified seeds, GA(4) and GA(4+7) being more effective than GA(3). Fluridone, an abscisic acid (ABA) biosynthesis inhibitor, was efficient in breaking dormancy, but it was less effective than GA(4) or GA(4+7) alone. Paclobutrazol, a GA biosynthesis inhibitor, inhibited seed germination, and the inhibitory effect was reversed completely by GA(4) and by GA(4+7). ABA content of seeds subjected to cold stratification or to incubation at 35/10 degrees C, which enhanced seed germination, was reduced about four- to sixfold compared to that of fresh seeds. Higher concentrations of GA(3), GA(4) and GA(7) were detected in nondormant seeds and in seeds with an emerged radicle than in fresh seeds. Present results seem to indicate that dormancy in P. amurense var. wilsonii seeds is imposed partially by the seed coat and partially by high ABA content. ABA content decreased and GA(3), GA(4) and GA(7) content increased during germination.

  13. Concentrations of p-synephrine in fruits and leaves of Citrus species (Rutaceae) and the acute toxicity testing of Citrus aurantium extract and p-synephrine.

    Science.gov (United States)

    Arbo, M D; Larentis, E R; Linck, V M; Aboy, A L; Pimentel, A L; Henriques, A T; Dallegrave, E; Garcia, S C; Leal, M B; Limberger, R P

    2008-08-01

    Dietary supplements containing bitter orange unripe fruit extract/p-synephrine are consumed worldwide for lose weight. This study were conducted to determine the concentration of p-synephrine in unripe fruits and leaves from Citrus aurantium Lin, C. sinensis Osbeck, C. deliciosa Ten, C. limon Burm and C. limonia Osbeck, collected in Southern Brazil, and to evaluate the acute toxicity of C. aurantium extract and p-synephrine. A high performance liquid chromatographic method with diode array detector (HPLC-DAD) was optimized and validated for determination of p-synephrine. The results indicate that all of analyzed samples present p-synephrine in amounts that range from 0.012% to 0.099% in the unripe fruits and 0.029 to 0.438% in the leaves. Acute oral administration of C. aurantium extracts (2.5% p-synephrine, 300-5,000 mg/kg) in mice produced reduction of locomotor activity, p-synephrine (150-2,000 mg/kg) produced piloerection, gasping, salivation, exophtalmia and reduction in locomotor activity, which was confirmed in spontaneous locomotor activity test. All the effects were reversible and persisted for 3-4h. The toxic effects observed seem to be related with adrenergic stimulation and should alert for possible side effects of p-synephrine and C. aurantium.

  14. Prevention of Dengue fever through plant based mosquito repellent Clausena dentata (Willd.) M. Roem (Family: Rutaceae) essential oil against Aedes aegypti l. (Diptera: Culicidae) mosquito.

    Science.gov (United States)

    Rajkumar, S; Jebanesan, A

    2010-03-01

    Plant based repellent against mosquito borne diseases are used recently because synthetic repellents cause side effects like breathing problem, eye irritation, head ache, cough, etc. The use of natural products for dengue control would protect the environment, reduce dependence on expensive synthetic repellents and also generate local employment. Essential oil was isolated by steam distillation which was used against the bites of Aedes aegypti and duration of protection period was assessed. Skin-irritant potential test was also conducted on 25 healthy volunteers by using four-point scale. The increase in the concentrations of essential oil increased the mean protection time against the bites of Aedes aegypti. The lowest mean protection time was 180.0 min for 2.5% and highest time of 255.0 min for 10%. The mean score of zero for skin-irritant potential test for all the concentrations indicated that the essential oil did not cause irritation to human skin. Results indicated that the use of plant based repellent for the control of dengue fever would replace the currently used synthetic repellents which causes many side effects.

  15. Comportamento fenológico no evento pós-queima e biologia reprodutiva de Spiranthera odoratissima A. St.-Hil. (Rutaceae

    Directory of Open Access Journals (Sweden)

    Cristiane Soares Pereira da Silva

    2011-09-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2008v21n1p29 O comportamento fenológico das espécies de cerrado influenciadas pela ação de queimadas é pouco conhecido. O estudo da fenologia e biologia reprodutiva de Spiranthera odoratissima A. St.-Hil. foi realizado em uma área antropizada de cerrado sensu stricto submetida ao fogo no município  de Goiânia, Goiás. Observações fenológicas evidenciaram que os indivíduos floresceram sincronicamente três meses após a queima. Os  ritmos fenológicos estiveram associados aos efeitos da sazonalidade pluviométrica, padrão característico das espécies subarbustivas de cerrado. As flores são brancas, de odor adocicado e estão reunidas em inflorescências paniculadas. A antese é crepuscular e inicia-se por volta das 16h. Os recursos oferecidos aos visitantes são pólen e néctar. A espécie produz 32,8μl (± 3,4 de néctar com concentração média de 16,4% (± 0,43 em equivalentes de sacarose. O sistema de polinização (falenofi lia foi proposto com base na análise das características florais. Os visitantes observados foram abelhas (Apis mellifera Linnaeus, moscas, vespas, formigas e besouros, porém, devido ao comportamento na inflorescência,  foram considerados apenas pilhadores de recursos. Foi constatado que Trigona spinipes Fabr. pode atuar como polinizador secundário durante a pilhagem de pólen. Os resultados das polinizações manuais e o índice de incompatibilidade (ISI indicam que a espécie é xenógama e auto-compatível.

  16. Functional characterization of acetylated Brazil nut (Bertholletia excelsa HBK kernel globulin Caracterizaçao funcional das globulinas de amêndoa de castanha-do-Pará após a acetilação

    Directory of Open Access Journals (Sweden)

    Cíntia Maria Pinto Ramos

    2004-03-01

    Full Text Available Defatted Brazil nut kernel flour, a rich source of high quality proteins, is presently being utilized in the formulation of animal feeds. One of the possible ways to improve its utilization for human consumption is through improvement in its functional properties. In the present study, changes in some of the functional properties of Brazil nut kernel globulin were evaluated after acetylation at 58.6, 66.2 and 75.3% levels. The solubility of acetylated globulin was improved above pH 6.0 but was reduced in the pH range of 3.0-4.0. Water and oil absorption capacity, as well as the viscosity increased with increase in the level of acetylation. Level of modification also influenced the emulsifying capacity: decreased at pH 3.0, but increased at pH 7.0 and 9.0. Highest emulsion activity (approximately 62.2% was observed at pH 3.0 followed by pH 9.0 and pH 7.0 and least (about 11.8% at pH 5.0. Emulsion stability also followed similar behavior as that of emulsion activity.Farinha desengordurada de amêndoa de castanha-do-Pará, fonte rica de proteína de alta qualidade, vem sendo, atualmente, aproveitada apenas na formulação de ração animal. Uma das possíveis maneiras de melhorar seu aproveitamento para o consumo humano é através do melhoramento de suas propriedades funcionais. No presente trabalho, mudanças em algumas propriedades funcionais da globulina de castanha-do-Pará, após acetilação, aos níveis de 58,6, 66,2 e 75,3% foram estudadas. A solubilidade da globulina acetilada aumentou acima de pH 6,0, porém diminuiu na faixa de pH 3,0 a 4,0. As capacidades de absorção de água e de óleo como também a viscosidade, melhoraram com o aumento de grau de acetilação. O grau de modificação também influenciou a capacidade de emulsificação: reduziu em pH 3,0, e aumentou nos pHs 7,0 e 9,0. A máxima atividade de emulsão (aproximadamente 62,2% foi observada em pH 3,0 seguida de pH 9,0 e a mínima foi observada (11,8% em pH 5,0. A estabilidade de emulsão também apresentou o comportamento similar à atividade de emulsão.

  17. Variações anuais na produção de frutos e sementes de Castanheira-do-Brasil (Bertholletia excelsa Bonpl., Lecythidaceae em florestas nativas de Roraima

    Directory of Open Access Journals (Sweden)

    Helio Tonini

    2014-02-01

    Full Text Available Este trabalho foi desenvolvido com o objetivo de estudar as variações anuais na produção de frutos e sementes entre populações e indivíduos em castanhais nativos de Roraima. Os dados foram obtidos em cinco parcelas permanentes de 300 m x 300 m instaladas nos Municípios de Caracaraí e São João da Baliza. Nas parcelas permanentes, todas as castanheiras com diâmetro à altura do peito (DAP superior ou igual a 10 cm foram identificadas, medidas e numeradas através de placas de alumínio presas aos fustes por pregos. Durante o período de queda dos frutos (março a julho, as árvores foram visitadas em duas ocasiões no meio e no final desse período, e os frutos contados, abertos e pesados com balança de gancho digital com precisão de 50 g. A produção de frutos e sementes varia entre populações e indivíduos e a maioria não produz frutos todos os anos. No ano de maior produção, um castanhal chega a produzir 52 vezes mais do que em anos de baixa. Nos locais estudados, a produção total concentra-se em um pequeno número de indivíduos com um ano de pico de produção (mast-year.

  18. NCBI nr-aa BLAST: CBRC-DDIS-06-0095 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DDIS-06-0095 gb|AAX19886.1| telomerase reverse transcriptase catalytic subunit [Doryanthes excel...sa] gb|AAX19887.1| telomerase reverse transcriptase catalytic subunit [Doryanthes excelsa] AAX19886.1 1e-44 25% ...

  19. Author Details - AJOL

    African Journals Online (AJOL)

    Antibacterial activity of aloe emodin and aloin A isolated from Aloe excelsa. Abstract PDF · Vol 5, ... Traditional use, antibacterial activity and antifungal activity of crude extract of Aloe excelsa ... The chemical composition and biological activities of essential oil from the fresh leaves of Schinus terebinthifolius from Zimbabwe

  20. A nuclear phylogenetic analysis: SNPs, indels and SSRs deliver new insights into the relationships in the 'true citrus fruit trees' group (Citrinae, Rutaceae) and the origin of cultivated species.

    Science.gov (United States)

    Garcia-Lor, Andres; Curk, Franck; Snoussi-Trifa, Hager; Morillon, Raphael; Ancillo, Gema; Luro, François; Navarro, Luis; Ollitrault, Patrick

    2013-01-01

    Despite differences in morphology, the genera representing 'true citrus fruit trees' are sexually compatible, and their phylogenetic relationships remain unclear. Most of the important commercial 'species' of Citrus are believed to be of interspecific origin. By studying polymorphisms of 27 nuclear genes, the average molecular differentiation between species was estimated and some phylogenetic relationships between 'true citrus fruit trees' were clarified. Sanger sequencing of PCR-amplified fragments from 18 genes involved in metabolite biosynthesis pathways and nine putative genes for salt tolerance was performed for 45 genotypes of Citrus and relatives of Citrus to mine single nucleotide polymorphisms (SNPs) and indel polymorphisms. Fifty nuclear simple sequence repeats (SSRs) were also analysed. A total of 16 238 kb of DNA was sequenced for each genotype, and 1097 single nucleotide polymorphisms (SNPs) and 50 indels were identified. These polymorphisms were more valuable than SSRs for inter-taxon differentiation. Nuclear phylogenetic analysis revealed that Citrus reticulata and Fortunella form a cluster that is differentiated from the clade that includes three other basic taxa of cultivated citrus (C. maxima, C. medica and C. micrantha). These results confirm the taxonomic subdivision between the subgenera Metacitrus and Archicitrus. A few genes displayed positive selection patterns within or between species, but most of them displayed neutral patterns. The phylogenetic inheritance patterns of the analysed genes were inferred for commercial Citrus spp. Numerous molecular polymorphisms (SNPs and indels), which are potentially useful for the analysis of interspecific genetic structures, have been identified. The nuclear phylogenetic network for Citrus and its sexually compatible relatives was consistent with the geographical origins of these genera. The positive selection observed for a few genes will help further works to analyse the molecular basis of the variability of the associated traits. This study presents new insights into the origin of C. sinensis.

  1. A nuclear phylogenetic analysis: SNPs, indels and SSRs deliver new insights into the relationships in the ‘true citrus fruit trees’ group (Citrinae, Rutaceae) and the origin of cultivated species

    Science.gov (United States)

    Garcia-Lor, Andres; Curk, Franck; Snoussi-Trifa, Hager; Morillon, Raphael; Ancillo, Gema; Luro, François; Navarro, Luis; Ollitrault, Patrick

    2013-01-01

    Background and Aims Despite differences in morphology, the genera representing ‘true citrus fruit trees’ are sexually compatible, and their phylogenetic relationships remain unclear. Most of the important commercial ‘species’ of Citrus are believed to be of interspecific origin. By studying polymorphisms of 27 nuclear genes, the average molecular differentiation between species was estimated and some phylogenetic relationships between ‘true citrus fruit trees’ were clarified. Methods Sanger sequencing of PCR-amplified fragments from 18 genes involved in metabolite biosynthesis pathways and nine putative genes for salt tolerance was performed for 45 genotypes of Citrus and relatives of Citrus to mine single nucleotide polymorphisms (SNPs) and indel polymorphisms. Fifty nuclear simple sequence repeats (SSRs) were also analysed. Key Results A total of 16 238 kb of DNA was sequenced for each genotype, and 1097 single nucleotide polymorphisms (SNPs) and 50 indels were identified. These polymorphisms were more valuable than SSRs for inter-taxon differentiation. Nuclear phylogenetic analysis revealed that Citrus reticulata and Fortunella form a cluster that is differentiated from the clade that includes three other basic taxa of cultivated citrus (C. maxima, C. medica and C. micrantha). These results confirm the taxonomic subdivision between the subgenera Metacitrus and Archicitrus. A few genes displayed positive selection patterns within or between species, but most of them displayed neutral patterns. The phylogenetic inheritance patterns of the analysed genes were inferred for commercial Citrus spp. Conclusions Numerous molecular polymorphisms (SNPs and indels), which are potentially useful for the analysis of interspecific genetic structures, have been identified. The nuclear phylogenetic network for Citrus and its sexually compatible relatives was consistent with the geographical origins of these genera. The positive selection observed for a few genes will help further works to analyse the molecular basis of the variability of the associated traits. This study presents new insights into the origin of C. sinensis. PMID:23104641

  2. In vitro Antifungal, Antioxidant and Cytotoxic Activities of a Partially ...

    African Journals Online (AJOL)

    Purpose: To determine the in vitro antifungal and antioxidant activities of the aqueous extract and protein fraction of Atlantia monophylla Linn (Rutaceae) leaf. Methods: Ammonium sulphate (0 – 80 %) precipitation method was used to extract protein from the leaves of A. monophylla Linn (Rutaceae). In vitro antifungal ...

  3. Essential oil composition and nutrient analysis of selected medicinal plants in Sultanate of Oman

    Directory of Open Access Journals (Sweden)

    Javid Hussain

    2013-12-01

    Full Text Available Objective: To evaluate the nutrients and essential oils of five medicinal plants, Juniperus excelsa (J. excelsa, Dodonaea viscosa, Euryops pinifolius, Teucrium polium (T. polium, and Helianthemum lippii that were collected from Jabal Al Akhdar, Oman. Methods: Proximate parameters (moisture, dry matter, ash, crude fats, proteins, fibers, nitrogen, carbohydrates, and energy values and nutrient analysis (K, Na, Ca, Fe, P, Mg etc. were evaluated in the five medicinal plants using standard techniques. On the basis of these analysis, T. polium and J. excels were selected for essential oil analysis using a rapid solvent-free microwave extraction method and GC-MS. Results: The results showed that leaves of J. excelsa had highest proportion of crude fats, fibers and energy value while ash was highest in T. polium. J. excelsa was also rich in essential minerals such as calcium, magnesium, potassium and iron while the trace elements and heavy metals composition was marginal. A rapid solvent-free microwave extraction method to extract oil from medicinal plants species showed that only T. polium and J. excelsa yielded oil. The chemical composition of essential oils showed higher proportion of delta-3-carene, limonene, β-eudesmol, ledeneoxide (II, α-trans-bergamatene, linalyl acetate and germacrene. Conclusions: J. excelsa and T. polium are a good source of proximate, minerals and essential oils, which can be considered for healthy life besides their medicinal values.

  4. Drug: D10235 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 571] ... Same as: E00338 ... Rutaceae (rue family) Citrus unshiu, Citrus reticulata immature fruit and fruit peel; Standards for non-pharmacopoeial crude drugs ... PubChem: 163312266 ...

  5. Wongkattiya et al., Afr J Tradit Complement Altern Med., (2018) 15 (2 ...

    African Journals Online (AJOL)

    2018-02-23

    Feb 23, 2018 ... The plant genus Zanthoxylum, which is in the family of Rutaceae, .... the essential oil at which the bacteria did not demonstrate visible growth. ... temperature, the absorbance was measured at a wavelength of 517 nm.

  6. Nitrogen–use efficiency in different vegetation type at Cikaniki Research Station, Halimun-Salak Mountain National Park, West Java

    Directory of Open Access Journals (Sweden)

    SUHARNO

    2007-10-01

    Full Text Available A research about nitrogen–use efficiency (NUE and trees identification was conducted at different vegetation type at Cikaniki, Halimun-Salak National Park, West Java. Plot quadrate methods (20 x 50 m was used to analyze trees vegetation and Kjeldahl methods was used to analyze leaf nitrogen. The width and length of the leaf was also measured to obtain the leaf surface area. The result showed that there are 61 individual trees which consisted of 24 species was identified. The species which have 5 highest important value are Altingia excelsa (64,657, Castanopsis javanica (39,698, Platea latifolia (27,684, Garcinia rostrata (21,151, and Schima walichii (16,049. Futhermore Eugenia lineata (13,967, Melanochyla caesa (12,241, Quercus lineata (10,766, platea excelsa (10,766 have lower important value. Other trees have important value less than 10. Morphological and nitrogen content analyze were done on 4 species : Quercus lineata, G. rostrata, A. excelsa, and E. lineata. Among them, Quercus lineata has highest specific leaf area (SLA (0,01153, followed by G. rostrata (0,00821, A. excelsa (0,00579, and E. lineata (0,00984 g/cm2. The highest number of stomata was found on A. excelsa (85,10/mm2, followed by E. lineata (74,40/mm2, Q. lineata (53,70/mm2, and G. rostrata (18,4 /mm2. The emergent species (A. excelsa and Q. lineata have higher nitrogen content than the underlayer species (G. rostrata and E. lineata. A. excelsa have highest nitrogen use efficiency (28,19% compare to E. lineata (23,81% , Q. lineata (19,09%, and G. rostrata (14,87%. Although not significant, emergen species have higher NUE than underlayer species.

  7. Triagem fitoquímica e avaliação das atividades trombolítica e citotóxica de Cecropia hololeuca Miq. (Urticaceae, Lippia alba (Mill. N.E.Br. ex P. Wilson (Verbenaceae e Zanthoxylum rhoifolium Lam (Rutaceae

    Directory of Open Access Journals (Sweden)

    João Victor Dutra GOMES1 ; Rafael Destefani FAITANIN

    2016-03-01

    Full Text Available Cecropia hololeuca, Lippia alba, e Zanthoxylum rhoifolium são espécies vegetais de ocorrência natural no Brasil, características de regiões de Mata Atlântica e, dentro de suas finalidades medicinais, estão incluídas ação anti-hipertensiva, digestiva e no tratamento de malária. Neste estudo, foram avaliados o perfil fitoquímico e as atividades citotóxica e trombolítica do extrato etanólico das folhas destas espécies vegetais. O perfil químico dos extratos foi obtido por meio de ensaios fitoquímicos clássicos, a fim de identificar as classes químicas presentes. A toxicidade preliminar foi avaliada frente Artemia salina e a atividade trombolítica foi determinada in vitro a partir da lise de coágulo de sangue humano. Os testes fitoquímicos indicaram a presença de alcaloides e flavonoides nos três extratos investigados. Saponinas, triterpenos e naftoquinonas foram detectados apenas em C. hololeuca. Essa não demonstrou toxicidade frente Artemia salina (DL50 >1000 ppm, enquanto Z. rhoifolium apresentou DL50 igual a 719,44 ppm e L. alba <250 ppm, o que indica atenção quanto à segurança no uso desta planta medicinal. A atividade trombolítica de L. alba e C. hololeuca foi de 6,43 ±2,08 e 9,64 ±1,83 %, respectivamente, mostrando baixa atividade. Por sua vez, Z. rhoifolium alcançou lise de 24,71 ±10,52 %, indicando promissora atividade (p <0,001. A atividade trombolítica de Z. rhoifolium justifica novos estudos, a fim de investigar os componentes responsáveis pela atividade. Perante a literatura, este é o primeiro relato da avaliação da atividade trombolítica destas espécies vegetais. Os resultados encontrados neste trabalho contribuem para o conhecimento químico-biológico das respectivas espécies.

  8. Absorption of some mineral salts by root system of different woody species and accumulation over a whole vegetative cycle (1963)

    International Nuclear Information System (INIS)

    Gagnaire, J.; Gerard, J.M.

    1963-01-01

    The concentration power of plant tissues and the translocation speed of mineral salts are considerably varying with the absorbed salt, the botanical species, the considered tissue and the part of the vegetative cycle. In Grenoble, with Picea excelsa, the 'true dormancy' is short (half-november, end of december). It is accompanied by a pre-dormancy period (October, half-november) and a post dormancy period (January, february, march). In vegetative period, Picea excelsa leaves are less concentrating mineral salt than Acer campestris leaves (coefficient 2 for calcium - 3 for phosphates) and Populus nigra leaves (coefficient 3 for calcium, coefficient 5 for phosphates). (author) [fr

  9. First Record of Diaphorina citri (Hemiptera: Psyllidae) in Ecuador Infesting Urban Citrus and Orange Jasmine Trees

    Science.gov (United States)

    Cornejo, J.F.; Chica, E.J.

    2014-01-01

    Abstract Adults and nymphs of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), were collected in the Guayaquil, Samborondón, and Durán cantons in coastal Ecuador. Psyllids were found in high numbers in citrus ( Citrus spp., Sapindales: Rutaceae) and orange jasmine ( Murraya exotica [L.] Jack, Sapindales: Rutaceae) trees within the Guayaquil-Samborondon-Duran conurbation; however, none was found during scoutings in the main citrus producing areas in coastal Ecuador. To the best of our knowledge, this is the first report of D. citri in Ecuador and the Pacific coastal plain of South America. PMID:25527601

  10. PRIMER REPORTE DE LA PRESENCIA DE Diaphorina citri (HEMIPTERA: LIVIIDAE EN MANABÍ

    Directory of Open Access Journals (Sweden)

    José Bernardo Navarrete Cedeño

    2016-12-01

    Full Text Available Diaphorina citri (Hemiptera: Liviidae, conocido como el psílido asiático de los cítricos, es considerada una plaga clave de la citricultura mundial, debido a que es vector de la bacteria “Candidatus Liberibacter”, agente causal de la enfermedad “Huanglongbing”, que tiene efectos letales sobre vegetales dentro de la familia Rutaceae. En este documento se reporta la presencia de D. citri infestando Murraya spp., un arbusto de la familia Rutaceae, en la zona urbana del cantón Portoviejo. Este es el primer reporte de la plaga en la provincia de Manabí-Ecuador

  11. Analysis of volatile compounds of Malaysian Tualang ( Koompassia ...

    African Journals Online (AJOL)

    Analysis of volatile compounds of Malaysian Tualang ( Koompassia excelsa ) honey using gas chromatography mass spectrometry. ... Other classes of chemical compounds detected included acids, aldehydes, alcohols, ketones, terpenes, furans and a miscellaneous group. Methanol yielded the highest number of extracted ...

  12. Download this PDF file

    African Journals Online (AJOL)

    sea region of turkey, and the assumption that the traditional uses of s. Excelsa might be .... doses, significantly attenuated the rise in serum level of ast, alt and total bilirubin, provoked by ccl4. Treatment ..... 45-55, crc press, florida. 3. Anderson ...

  13. Long-term changes in structure and composition following hurricanes in a primary lower montane rain forest in Puerto Rico

    Science.gov (United States)

    P.L. Weaver

    2013-01-01

    Ridges within the lower montane rain forests (sensu Beard) of the Caribbean Basin are dominated by Dacryodes excelsa, a tree species known as tabonuco in Puerto Rico and gommier in the Lesser Antilles. Periodially, hurricanes traverse the islands causing changes in structure, species composition, and dynamics of forests. The chronology of post-hurricane vegetation...

  14. Flowering Trees

    Indian Academy of Sciences (India)

    IAS Admin

    Flowering Trees. Ailanthus excelsa Roxb. (INDIAN TREE OF. HEAVEN) of Simaroubaceae is a lofty tree with large pinnately compound alternate leaves, which are ... inflorescences, unisexual and greenish-yellow. Fruits are winged, wings many-nerved. Wood is used in making match sticks. 1. Male flower; 2. Female flower.

  15. Penicillium excelsum sp. nov from the Brazil Nut Tree Ecosystem in the Amazon Basin'

    DEFF Research Database (Denmark)

    Taniwaki, Marta Hiromi; Pitt, John I; Iamanaka, Beatriz T.

    2015-01-01

    A new Penicillium species, P. excelsum, is described here using morphological characters, extrolite and partial sequence data from the ITS, β-tubulin and calmodulin genes. It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as ...

  16. Aspergillus bertholletius sp. nov. from Brazil Nuts

    DEFF Research Database (Denmark)

    Taniwaki, Marta H.; Pitt, John I.; Iamanaka, Beatriz T.

    2012-01-01

    During a study on the mycobiota of brazil nuts (Bertholletia excelsa) in Brazil, a new Aspergillus species, A. bertholletius, was found, and is described here. A polyphasic approach was applied using morphological characters, extrolite data as well as partial beta-tubulin, calmodulin and ITS sequ...

  17. Bulletin of Materials Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Bulletin of Materials Science. V Vinmathi. Articles written in Bulletin of Materials Science. Volume 38 Issue 3 June 2015 pp 625-628. A green and facile approach for the synthesis of silver nanoparticles using aqueous extract of Ailanthus excelsa leaves, evaluation of its antibacterial and anticancer efficacy.

  18. Nursery Pest Management of Phytolyma lata Walker (Scott) Attack ...

    African Journals Online (AJOL)

    The establishment of plantations of Milicia excelsa has been constrained by the gall-forming psyllid Phytolyma lata Walker (Scott) that causes extensive damage to young plants. We present findings of an experiment aimed at preventing Phytolyma attack on Milicia seedlings in the nursery using chemical control and ...

  19. The effects of different pot length and growing media on seedling ...

    African Journals Online (AJOL)

    The aim of this study was to determine appropriate pot length and growing medium for Crimean Juniper seedlings (Juniperus excelsa Bieb.), which will be used for afforestation of extreme areas. For this purpose, polyethylene pots of 11 cm width and 20, 25 and 30 cm lengths were used. As growing medium, 13 different ...

  20. Biodiversity and human activities in the Udzungwa Mountain forests ...

    African Journals Online (AJOL)

    It was established that local communities around the forest highly depend on the natural forests for forest products. Most human uses were for traditional medicine, fuelwood and building materials. Quality hardwoods Khaya anthotheca, Afzelia quanzensis, Milicia excelsa and Ocotea usambarensis were noted. To reduce ...

  1. Antibacterial activity of aloe emodin and aloin A isolated from Aloe ...

    African Journals Online (AJOL)

    GRACE

    2006-06-02

    Jun 2, 2006 ... Aloe emodin and aloin A are known to occur in both commercial viable species of aloes (Aloe vera and A. ferox), but has not previously been isolated from A. excelsa. Although extensive research had been done with most Aloe species including chemical work. (Speranza et al., 1986; Speranza et al., 1990; ...

  2. Browse Title Index

    African Journals Online (AJOL)

    Items 151 - 200 of 1250 ... J Zhou, F Qu. Vol 10, No 2 (2013), Analysis of volatile compounds of Malaysian Tualang (Koompassia excelsa) honey using gas chromatography mass spectrometry, Abstract PDF. SMS Nurul, SH Gan, AS Halim, NSM Shah, SH Gan, HA Sukari. Vol 10, No 4 (2013), Analysis on 113 cases of adverse ...

  3. Wood anatomy of the neotropical Sapotaceae : XVII. Gambeya

    Science.gov (United States)

    Bohumil Francis Kukachka

    1980-01-01

    Gambeya is an African genus to which Aubréville added Gambeya excelsa (Huber) Aubr., based on the Amazonian Chrysophyllum excelsum Huber. Whether Gambeya is the appropriate taxon for the American species remains to be resolved. Wood specimens indicate that several species occur in the Americas ranging from Southern Mexico to the Peruvian Amazon. The woods of the...

  4. First record of Anastrepha serpentina (Wiedemann) (Diptera: Tephritidae) in citrus in Brazil.

    Science.gov (United States)

    Lemos, W P; da Silva, R A; Araújo, S C A; Oliveira, E L A; da Silva, W R

    2011-01-01

    Anastrepha serpentina (Wiedemann) is recorded for the first time in citrus (Rutaceae) in Brazil. Specimens were obtained from sweet orange (Citrus sinensis) sampled in the municipalities of Belém and Capitão Poço, and from mandarin orange (Citrus reticulata) from Tomé-Açu, state of Pará, Brazil.

  5. First record of Anastrepha serpentina (Wiedemann) (Diptera: Tephritidae) in citrus in Brazil

    OpenAIRE

    Lemos, WP; Silva, RA da; Araújo, SCA; Oliveira, ELA; Silva, WR da

    2011-01-01

    Anastrepha serpentina (Wiedemann) is recorded for the first time in citrus (Rutaceae) in Brazil. Specimens were obtained from sweet orange (Citrus sinensis) sampled in the municipalities of Belém and Capitão Poço, and from mandarin orange (Citrus reticulata) from Tomé-Açu, state of Pará, Brazil.

  6. Effect of chemical compounds on the ‘Cadidatus Liberibacter asiaticus’ infected pomelo (Citrus maxima)

    Science.gov (United States)

    Huanglongbing (HLB), also known as citrus greening, is one of the most destructive diseases affecting Rutaceae plants in many parts of the world. HLB is associated with three species of ‘Candidatus Liberibacter’ with ‘Ca. L. asiaticus’ (Las), being the most widely distributed in Thailand and Asia. T...

  7. First report of Phyllosticta citricarpa and description of two new species, P. paracapitalensis and P. paracitricarpa, from citrus in Europe

    NARCIS (Netherlands)

    Guarnaccia, V.; Groenewald, J.Z.; Li, H.; Glienke, C.; Carstens, E.; Hattingh, V.; Fourie, P.H.; Crous, P.W.

    2017-01-01

    Abstract The genus Phyllosticta occurs worldwide, and contains numerous plant pathogenic, endophytic and saprobic species. Phyllosticta citricarpa is the causal agent of Citrus Black Spot disease (CBS), affecting fruits and leaves of several citrus hosts (Rutaceae), and can also be isolated from

  8. antibacterial activities of the volatile oil and aqueous extract of ...

    African Journals Online (AJOL)

    The volatile oil of Murraya keonigii was active against Staphylococcus epidemidis, S. aureus, and Streptococcus specie while the aqueous extract was not active. Key Words: Murraya koenigii, Rutaceae, antibacterial activity. Nig. J. Nat. Prod. And Med. Vol.2 1998: 44-45 ...

  9. Chemical Constituents and Biological Activities of Zanthoxylum ...

    African Journals Online (AJOL)

    Zanthoxylum limonella belongs to the family of aromatic deciduous trees and shrubs, Rutaceae. In traditional medicine practice, various parts of Z. limonella are used for the treatment of dental caries, febrifugal, sudorific, rheumatism, diuretic, stomach ache and diarrhea. Secondary metabolites have been isolated the stems, ...

  10. In vivo anti-malarial potentials of some plants extracts on ICR-mice ...

    African Journals Online (AJOL)

    Five medicinal plants, Acacia nilotica (Fabaceae), Citrus aurantifolia (Rutaceae), Mangifera indica (Anacardiaceae) Carica papaya (Caricaceae), and Psidium guajava (Myrtaceae) used for the treatment of malaria/ fever by the Hausa people of Kano-Nigeria were selected based on their traditional claims. These were ...

  11. 10 - 18_Aworinde

    African Journals Online (AJOL)

    DR. AMINU

    From the data, Euphorbiaceae,. Solanaceae, Rutaceae, Malvaceae, Caesalpiniaceae, Poaceae and Apocynaceae (in order of decreasing number of species) were the most frequent Families. Taxa such as Musa species,. Vernonia amygdalina, Citrus species, Psidium guajava and Terminalia catappa were found to be the.

  12. Fulltext PDF

    Indian Academy of Sciences (India)

    Admin

    Clausena anisata (Willd.) Hook.f. ex Benth. (Syn. C.dentata (Willd.) Roemer) – Black Currant. Grape Lime of the family Rutaceae is a small aromatic evergreen tree. Leaves are odd-pinnate with elliptic-ovate and oblique leaflets. Inflorescence is a raceme and flowers are greenish-white, and. 4-merous. Fruit is an ovoid small ...

  13. Chemical composition, cytotoxicity and antioxidant activities of the ...

    African Journals Online (AJOL)

    The species of the genus Citrus (Rutaceae) have been widely used in traditional medicine. In this study, the essential oil was extracted from the leaves of Citrus aurantium and its cytotoxicity effect on six tumor cell lines and a normal cell line was studied. Furthermore, antioxidant potential of the oil was tested by 2, ...

  14. Chemical Composition of Zanthoxylum avicennae Essential Oil and ...

    African Journals Online (AJOL)

    Purpose: To determine the larvicidal activity of the essential oil derived from Zanthoxylum avicennae (Lam.) DC. (Rutaceae) leaves and stems against the larvae of Aedes albopictus Skuse. Methods: Essential oil of Z. avicennae leaves and stems were obtained by hydrodistillation and analyzed by gas chromatography ...

  15. Simultaneous determination of aegeline and six coumarins from different parts of the plant Aegle marmelos using UHPLC-PDA-MS and chiral separation of aegeline using HPLC-ToF-MS

    Science.gov (United States)

    A fast UHPLC-PDA method was developed for the simultaneous analysis of one alkaloid, aegeline, and six coumarins namely: umbelliferone; scopoletin; marmesinin; 8-hydroxypsoralen angelicin and marmelosin from leaf, fruit, root and bark of Aegle marmelos (L.) Corrêa (Rutaceae). The method was validate...

  16. Use of micro-CT to elucidate details of the anatomy and feeding of the Asian citrus psyllid Diaphorina citri Kuwayama, 1908 (Insecta: Hemiptera, Liviidae)

    Science.gov (United States)

    Huanglongbing (HLB), also known as citrus greening disease, is caused by plant-infecting bacteria. The most prominent pathogen within the Americas: United States of America, Mexico, and Brazil, is Candidatus Liberibacter asiaticus, which affects plants of the Family: Rutaceae, in particularly citrus...

  17. Research in the Botany Department. University of Malaya, Kuala Lumpur

    NARCIS (Netherlands)

    NN,

    1969-01-01

    Dr. B.C. Stone, the present Head of the Botany Unit, is continuing his investigations on Pandanaceae, which form the major research work; and on Rutaceae and Araliaceae, two other families which are his favorites. The genus Freycinetia is the nearest to completion; it is expected to have about

  18. Fulltext PDF

    Indian Academy of Sciences (India)

    Feronia limonia (L.) S~villg/e (B. Koet Bel) (English: Elephant- or Wood-apple; Hindi: Kaithbel, Kaitha,. Kavitha) of Rutaceae is a medium-sized deciduous tree with thorny branches and compound leaves. Flowers are small and white. Fruits are large, globose, of the size of tennis balls with a hard and woody shell enclosing ...

  19. Aspergillus bertholletius sp. nov. from Brazil Nuts

    Science.gov (United States)

    Taniwaki, Marta H.; Pitt, John I.; Iamanaka, Beatriz T.; Sartori, Daniele; Copetti, Marina V.; Balajee, Arun; Fungaro, Maria Helena P.; Frisvad, Jens C.

    2012-01-01

    During a study on the mycobiota of brazil nuts (Bertholletia excelsa) in Brazil, a new Aspergillus species, A. bertholletius, was found, and is described here. A polyphasic approach was applied using morphological characters, extrolite data as well as partial β-tubulin, calmodulin and ITS sequences to characterize this taxon. A. bertholletius is represented by nineteen isolates from samples of brazil nuts at various stages of production and soil close to Bertholletia excelsa trees. The following extrolites were produced by this species: aflavinin, cyclopiazonic acid, kojic acid, tenuazonic acid and ustilaginoidin C. Phylogenetic analysis using partial β-tubulin and camodulin gene sequences showed that A. bertholletius represents a new phylogenetic clade in Aspergillus section Flavi. The type strain of A. bertholletius is CCT 7615 ( = ITAL 270/06 = IBT 29228). PMID:22952594

  20. Aspergillus bertholletius sp. nov. from Brazil nuts.

    Directory of Open Access Journals (Sweden)

    Marta H Taniwaki

    Full Text Available During a study on the mycobiota of brazil nuts (Bertholletia excelsa in Brazil, a new Aspergillus species, A. bertholletius, was found, and is described here. A polyphasic approach was applied using morphological characters, extrolite data as well as partial β-tubulin, calmodulin and ITS sequences to characterize this taxon. A. bertholletius is represented by nineteen isolates from samples of brazil nuts at various stages of production and soil close to Bertholletia excelsa trees. The following extrolites were produced by this species: aflavinin, cyclopiazonic acid, kojic acid, tenuazonic acid and ustilaginoidin C. Phylogenetic analysis using partial β-tubulin and camodulin gene sequences showed that A. bertholletius represents a new phylogenetic clade in Aspergillus section Flavi. The type strain of A. bertholletius is CCT 7615 ( = ITAL 270/06 = IBT 29228.

  1. Physical, Chemical, and Biological Properties of Soil under Decaying Wood in a Tropical Wet Forest in Puerto Rico

    Science.gov (United States)

    Marcela Zalamea; Grizelle Gonzalez; D. Jean Lodge

    2016-01-01

    Decaying wood is related to nutrient cycling through its role as either a sink or source of nutrients. However, at micro scales, what is the effect of decaying logs on the physical, chemical,and biotic characteristics of the soil underneath? We took samples from a 0 to 5 cm depth under and a 50 cm distance away from decaying logs (Dacryodes excelsa and Swietenia...

  2. A taxonomic review of the genus Antepipona de Saussure, 1855 (Hymenoptera: Vespidae: Eumeninae) from India.

    Science.gov (United States)

    Kumar, Girish P; Carpenter, James M; Sureshan, Pavittu M

    2016-08-16

    A review of 20 Indian species of the genus Antepipona de Saussure with a key to species and subspecies is provided. A new synonymy is proposed for Antepipona biguttata (Fabricius, 1787) =A. excelsa keralensis Lambert, 2004, syn. nov. The parasitic association of Strepsiptera is reported for the first time in the genus Antepipona. Antepipona rufescens (Smith, 1857) is newly recorded from India and A. ovalis (de Saussure, 1853) from Bangladesh. The reported distributions of ten species within India are enlarged.

  3. Origin and domestication of native Amazonian crops

    OpenAIRE

    Clément, R. Charles; De Cristo-Araujo, Michelly; Coppens D'Eeckenbrugge, Géo; Alves Pereira, Allessandro; Picanço-Rodrigues, Doriane

    2010-01-01

    Molecular analyses are providing new elements to decipher the origin, domestication and dispersal of native Amazonian crops in an expanding archaeological context. Solid molecular data are available for manioc (Manihot esculenta), cacao (Theobroma cacao), pineapple (Ananas comosus), peach palm (Bactris gasipaes) and guaraná (Paullinia cupana), while hot peppers (Capsicum spp.), inga (Inga edulis), Brazil nut (Bertholletia excelsa) and cupuassu (Theobroma grandiflorum) are being studied. Emerg...

  4. Animals and trees: food for thought

    Energy Technology Data Exchange (ETDEWEB)

    Openshaw, K.

    1979-01-01

    In many areas of Africa, combining tree-growing with animal rearing is advantageous, as the trees provide shade, animal fodder and timber for fuel and building, while grazing animals reduce the fire hazard from ground vegetation and improve soil fertility through droppings. Acacia albida, Prosopis cineraria, P. chilensis, leucaena leucocephala and Ailanthus excelsa are discussed as promising fodder trees, and an appendix is included with notes on 21 other trees for fodder or the production of medicines.

  5. Distribution of Sandflies (Diptera:Psychodidae on Tree-trunks in a Non-flooded Area of the Ducke Forest Reserve, Manaus, AM, Brazil

    Directory of Open Access Journals (Sweden)

    MRS Cabanillas

    1999-05-01

    Full Text Available Sandflies were collected in the base of tree-trunks in the seasons of high and least rainfall in the Ducke Forest Reserve, near Manaus in the State of Amazonas. Lutzomyia umbratilis was the most abundant sandfly species. Caryocar villosum, Chrysophyllum amazonicum, Dinizia excelsa, Eschweilera atropetiolata and Parkia multijuga were the tree species on which most sandflies were collected and relative abundance were related to trunk characteristics. Seasonal patterns of sandfly distribution in the forest were observed.

  6. Potential of aqueous ozone to control aflatoxigenic fungi in Brazil nuts

    OpenAIRE

    Silva, Otniel Freitas; Morales-Valle, H.; Venâncio, Armando

    2011-01-01

    The Brazil nut (Bertholethia excelsa) is an important non timber forest product (NTFP) from the Amazonian forest. Despite their nutritious value, Brazil nuts are susceptible to contamination with Aspergillus section Flavi fungi and consequently with aflatoxins. Since aqueous ozone reduces microorganisms population and has oxidant effect on aflatoxins, the effect of ozone on. Both natural and artificially contaminated Brasil nuts were studied in the present work. The former were inoculated wit...

  7. Morfologia, comportamento, parasitismo e mecanismos de defesa dos imaturos de Heraclides anchisiades capys (Hübner(Lepidoptera, Papilionidae Morphology, behaviour, parasitism and mechanisms of defense of the immatures of Heraclides anchisiades capys (Hübner (Lepidoptera, Papilionidae

    Directory of Open Access Journals (Sweden)

    Luis Anderson Ribeiro Leite

    2010-06-01

    Full Text Available Heraclides anchisiades capys é uma espécie comum de Papilionidae, cujas larvas se alimentam de várias espécies de Citrus spp. (Rutaceae. Neste estudo são descritas a morfologia e dados sobre a história natural dos imaturos através de ilustrações, incluindo fotografias em microscopia eletrônica de varredura.Heraclides anchisiades capys is a common species among the Papilionidae, whose larvae feed on various species of Citrus spp. (Rutaceae. In this study the morphology and data on natural history of the immature stages of this species are described with illustrations, including photos in scanning electronic microscope.

  8. 1100-IJBCS-Article-Abiodoum Olounlade

    African Journals Online (AJOL)

    DR GATSING

    (Bignoniaceae) et de Zanthoxylum zanthoxyloïdes (Rutaceae), leur effet inhibiteur a été évalué in vitro sur la migration larvaire de ... Les extraits de Newbouldia laevis et de Zanthoxylum zanthoxyloïdes inhibent in vitro la migration larvaire de. Haemonchus contortus. ... médicaments, à la malfaçon et la contrefaçon de.

  9. Coumarins and alkaloids in shoot culture of Ruta graveolens L.

    Directory of Open Access Journals (Sweden)

    Halina Ekiert

    2014-01-01

    Full Text Available A shoot culture of Ruta graveolens L. (Rutaceae was maintained in the stationary liquid phase. From the cultured shoots seven compounds were isolated and identified as psoralen, bergapten, xanthotoxin, isopimpinellin (linear furanocoumarins, rutamarin (linear dihydrofuranocoumarin, kokusaginine and skimmianine (furanoquinoline alkaloids by spectral methods. The compounds are known as secondary metabolites of the intact plant, as well as its cell and tissue cultures.

  10. 377 Konan et al., Afr J Tradit Complement Altern Med. (2011) 8(4 ...

    African Journals Online (AJOL)

    AJTCAM

    aurantifolia (Rutaceae) sur le muscle lisse, Taenia coli de cobaye. Rev. Med. Pharm. Afr. 16: 103-112. 35. Souza, A., Aka, K.J., Abo, K.J.C., Datté, Y.J., Traoré, F. and M'Batchi, B. (2007). Myostimulating effect of the aqueous extract of Khaya senegalensis (Desr) A. Juss (Meliaceae) in isolated Taenia caeci contractile activity.

  11. ORANGE: RANGE OF BENEFITS

    OpenAIRE

    Parle Milind; Chaturvedi Dev

    2012-01-01

    No wonder that oranges are one of the most popular fruits in the world. Orange (citrus sinensis) is well known for its nutritional and medicinal properties throughout the world. From times immemorial, whole Orange plant including ripe and unripe fruits, juice, orange peels, leaves and flowers are used as a traditional medicine. Citrus sinensis belongs to the family Rutaceae. The fruit is a fleshy, indehiscent, berry that ranges widely in size from 4 cm to 12 cm. The major medicinal proper...

  12. Evaluation of furocoumarins as photosynthetic inhibitor by chlorophyll a fluorescence assay

    OpenAIRE

    Sampaio, Olívia Moreira; Silva, Maria Fátima das Graças Fernandes da; Veiga, Thiago Andre Moura [UNIFESP; King-Díaz, Beatriz; Lotina-Hennsen, Blas

    2012-01-01

    The evaluations of Chorophyll a fluorescence emitted by superior plants carry structural information and photosynthetic apparatus function. Quantitative analysis apparatus of fluorescence kinetic were measured by energy flows (ABS), (TR), (ET) and (DI), known as phenomenological phenomena of OJIP test. Four furocoumarins were isolated from Ruta graveolens (Rutaceae), and chorophyll a (Chl a) fluorescence assays were performed with these compounds to evaluate the photosynthesis inhibition pote...

  13. Antioxidant activity and antiaging gel formulation grapefruit peel (Citrus maxima Merr.) ethanolic extract

    OpenAIRE

    Nazliniwaty; Karsono; Zebua, Nilsya Febrika; Febrika, Nilsya

    2017-01-01

    This study aims to conduct the antioxidant activity test of grapefruit peel ethanolic extracts and gel formulation. Grapefruit (Citrus maxima Merr.) is a plant of the Rutaceae family, which has been known to contain ph enolic compounds (flavonoids and tannins). Grapefruit skin was very thick (>30% of the total weight of the fr uit) and always considered as waste that has not been utiliz ed properly....

  14. Phytochemical Screening, Antifungal and Antibacterial Effect of Zanthoxylum zanthoxyloides and Zanthoxylum macrophylum Used in Traditional Medicine in Yamboro (Central African Republic)

    OpenAIRE

    E. Kosh-Komba; L. Aba Toumnou; I. Zinga; I. Touckia

    2017-01-01

    The knowledge of traditional medicine has always guided the search for new cures. Zanthoxylum zanthoxyloides and Zanthoxylum macrophylum, (Rutaceae) are used as medicinal plants in Central African Republic for bacteria and fungi treatment. The aim of this study is to investigate in vitro antibacterial and antifungal activities of water-alcohol extract of leave, bark and root of Z. zanthoxyloides and Z. macrophylum and the phytochemistry group of some secondary metabolic. The results of an...

  15. Environ: E00063 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00063 Phellodendron bark (JP17) Crude drug Berberine [CPD:C00757], Palmatine [CPD...:C05315], Magnoflorine [CPD:C09581], Phellodendrine [CPD:C17046], Jateorrhizine [CPD:C09553], Obakunone [CPD...75], Menisperine, 7-Dehydrostigmasterol Phellodendron amurense [TAX:68554], Phellodendron chinense [TAX:3545...08] Same as: D06689 Rutaceae (rue family) Phellodendron bark Major component: Berberine [CPD:C00757] ...

  16. Composition of the Essential oil of Endemic Haplophyllum megalanthum Bornm. from Turkey

    Directory of Open Access Journals (Sweden)

    Nehir Ünver-Somer

    2012-01-01

    Full Text Available The composition of the essential oil produced from the flowering aerial parts of Haplophyllum megalanthum Bornm. (Rutaceae, endemic to Turkey, was analyzed by GC and GC-MS. Among the fifty-eight compounds constituting about 91.7 % of the essential oil, the main components were characterized as palmito- g -lactone (45.8 %, octadecatrienoic acid (10.7 %, linoleic acid (6.5 %, octadecatetraenoic acid (6.3 % and nonacosane (4.8 %.

  17. Morfologia, comportamento, parasitismo e mecanismos de defesa dos imaturos de Heraclides anchisiades capys (Hübner)(Lepidoptera, Papilionidae)

    OpenAIRE

    Leite,Luis Anderson Ribeiro; Casagrande,Mirna Martins; Mielke,Olaf Hermann Hendrik

    2010-01-01

    Heraclides anchisiades capys é uma espécie comum de Papilionidae, cujas larvas se alimentam de várias espécies de Citrus spp. (Rutaceae). Neste estudo são descritas a morfologia e dados sobre a história natural dos imaturos através de ilustrações, incluindo fotografias em microscopia eletrônica de varredura.

  18. Antibacterial activity of methanol extract of Ruta chalapensis (L), Quercus infectoria (Oliver) and Canthium parviflorum (Lam)

    Science.gov (United States)

    Priya, P. Sathiya; Sasikumar, J.M.; Gowsigan, G.

    2009-01-01

    The present study aimed at evaluating the antibacterial activity of methanol extract of Ruta chalapensis, L., (Rutaceae), Quercus infectoria Oliver., (Fagaceae) and Canthium parviflorum Lam., (Rubiaceae) against Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, Klebsiella oxytocoa, Klebsiella pneumoniae and Proteus mirabilis. The experiment was carried out using disc diffusion method. The results revealed that the methanol extract of aerial parts of Ruta chalepensis (L) presented the highest zone of inhibition against tested pathogens. Other plants showed significant zone of inhibition. PMID:22557348

  19. The effects of host, geographic origin, and gender on the thermal requirements of Diaphorina citri (Hemiptera: Psyllidae).

    Science.gov (United States)

    Nava, Dori E; Gomez-Torres, Mariuxi L; Rodrigues, Marjorie D; Bento, José M S; Haddad, Marinéia L; Parra, José R P

    2010-04-01

    Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is the vector of the bacteria that causes citrus greening and is considered one of the world's most important citrus diseases. We examined how host, geographic region, and gender affect the thermal requirements of D. citri. The insects were reared in climatic chambers at constant temperatures of 18, 20, 22, 25, 28, 30, and 32 +/- 1 degrees C, 70 +/- 10% RH, and a 14 h photophase. Host plants for D. citri included orange (Citrus sinensis [Rutaceae]) varieties Pêra and Natal, the rootstock, Rungpur lime (C. limonia [Rutaceae]) and the natural host, Orange jessamine (Murraya paniculata [Rutaceae]). To study the influence of geographic origin on thermal requirements, we studied D. citri populations from Piracicaba, SP (warmer region) and Itapetininga, SP (cooler region). The duration and survival of the development stages and the duration of the total development (egg-adult) did not differ significantly on the different hosts, but it did vary with temperature. Nymphs of D. citri created on the different hosts have the same thermal requirements. The thermal requirements for this species collected from the two climate regions were identical; males and females also had the same thermal requirements.

  20. Development and Evaluation of Herbal Formulations for Hair Growth

    Directory of Open Access Journals (Sweden)

    Lipi Purwal

    2008-01-01

    Full Text Available Hair formulation of Emblica officinalis (Euphorbiaceae, Bacopa, monnieri (Scrophulariaceae, Trigonella foenumgraecum (Leguminosae, Murraya koenigii (Rutaceae in various concentrations in the form of herbal oil were studied for their hair growth activity. Each drug was tested for their hair growth activity in a concentration range for 1-10% separately. Based on these results mixture of crude drugs Murraya koeniigi, leaf (Rutaceae, Bacopa monnieri, leaf (Scrophulariaceae, Trigonella foenumgraecum (Leguminosae, Murraya koenigii (Rutaceae were prepared in varying concentration in the form of herbal hair oil by three different oils preparation techniques and were tested for hair growth activity. The result revealed that the hair growth activity of each drug was found proportional to the concentration range tested. Similarly higher concentrations of drug in the formulation were found to have higher hair growth activities. But looking towards the formulation viscosity the maximum concentration of combined drug was found to be 30% at their maximum level. The formulation containing 7.5% of each drug used for the study and showed excellent hair growth activity with standard (2% minoxidil ethanolic solution by an enlargement of follicular size and prolongation of the anagen phase. It holds the promise of potent herbal alternative for minoxidil. Excellent results of hair growth were seen in formulation prepared by cloth pouch decoction method of oils preparation technique.

  1. New Limonoids from Hortia oreadica and Unexpected Coumarin from H. superba Using Chromatography over Cleaning Sephadex with Sodium Hypochlorite

    Directory of Open Access Journals (Sweden)

    Vanessa G.P. Severino

    2014-08-01

    Full Text Available Previous investigations of H. oreadica reported the presence of a wide spectrum of complex limonoids and dihydrocinnamic acids. Our interest in the Rutaceae motivated a reinvestigation of H. oreadica, H. brasiliana and H. superba searching for other secondary metabolites present in substantial amounts for taxonomic analysis. In a continuation of the investigation of the H. oreadica, three new limonoids have now been isolated 9α-hydroxyhortiolide A, 11β-hydroxyhortiolide C and 1(S*-acetoxy-7(R*-hydroxy-7-deoxoinchangin. All the isolated compounds from the Hortia species reinforce its position in the Rutaceae. With regard to limonoids the genus produces highly specialized compounds, whose structural variations do not occur in any other member of the Rutaceae, thus, it is evident from limonoid data that Hortia takes an isolated position within the family. In addition, H. superba afforded the unexpected coumarin 5-chloro-8-methoxy-psoralen, which may not be a genuine natural product. Solid-state cross-polarisation/magic-angle-spinning 13C nuclear magnetic resonance, X-Ray fluorescence and Field-emission gun scanning electron microscopy experiments show that the Sephadex LH-20 was modified after treatment with NaOCl, suggesting that when xanthotoxin (8-methoxy-psoralen was extracted from cleaning of the gel column, chlorination of the aromatic system occurred.

  2. Chloroplastid pigment contents and chlorophyll a fluorescence in Amazonian tropical three species Concentração de pigmentos cloroplastídicos e fluorescência da clorofila a em espécies arbóreas tropicais da Amazônia

    Directory of Open Access Journals (Sweden)

    Ronaldo Ribeiro de Morais

    2007-10-01

    Full Text Available Plants react to changes in light and hydrological conditions in terms of quantity and composition of chloroplastidic pigments, which affects the photosynthetic properties and consequently the accumulation of plant biomass. Thus, the chloroplastidic pigment concentration and chlorophyll a fluorescence of three Amazonian species (Bertholletia excelsa, Carapa guianensis e Dipteryx odorata were investigated in sun and shade leaves form the tree crown collected during two distinct periods of precipitation (dry and rainy seasons. Pigment contents were determined by spectrophotometry and fluorescence variables were determined using a portable fluorometer. The results demonstrated that the species showed high concentrations of Chl a, Chl b e Chl total during the wet season in relation to the dry season, especially in shade leaves. A higher concentration of carotenoids was found in B. excelsa, when compared with leaves of C. guianensis and D. odorata. In leaves of B. excelsa and D. odorata no significant difference was found in relation to the photochemistry of photosystem II (Fv/Fm between the wet and dry seasons. In conclusion, the three species react differently to variations in the light and precipitation conditions regarding light capture, aspects that might be considered in the management of forest plantations.As plantas respondem a mudanças nas condições de luz e na disponibilidade hídrica em termos da quantidade e composição dos pigmentos cloroplastídicos, o que afeta as propriedades fotossintéticas e, conseqüentemente, o acúmulo de biomassa das espécies. Assim, a concentração de pigmentos cloroplastídicos e a fluorescência da clorofila a de três espécies arbóreas (Bertholletia excelsa, Carapa guianensis e Dipteryx odorata da Amazônia foi investigada em folhas sombreadas e não-sombreadas da copa das árvores e em dois períodos distintos de precipitação (chuvoso e seco. As concentrações de pigmentos foram determinadas por

  3. ANALYSIS OF DWARF MISTLETOE ARCEUTHOBIUM OXYCEDRI (DC. M. BIEB. AND ITS PRINCIPAL HOST EASTERN PRICKLY JUNIPER JUNIPERUS DELTOIDES R. P. ADAMS DISTRIBUTION IN CRIMEA USING GIS TECHOLOGIES

    Directory of Open Access Journals (Sweden)

    O. V. Kukushkin

    2017-03-01

    Full Text Available The current study highlights the distribution pattern of juniper dwarf mistletoe (Arceuthobium oxycedri, a semi-parasite of the Eastern prickly juniper (Juniperus deltoides, in Crimea. A. oxycedri has considerably narrower range in Crimea as compared to its principal host and its ubiquitous distribution is rather sporadic. Nature observations characterize A. oxycedri as a thermophilic and mezo-хerophytic species confined to the low-mountain terrains with mild sub-Mediterranean climate. Significant sites of permanent infection have been discovered at the Crimean coast and in the warmest southwestern part of the Crimean Mountains to the south from the Belbek River valley. Greek juniper (J. excelsa is a codominant species growing side by side with J. deltoids in the majority of localities examined that have the high infection rate. Generally, J. excelsa is an insusceptible species in relation to the parasite; nevertheless, it is affected by A. oxycedri at several sites. Birds feeding habit to consume J. excelsa and J. deltoides fleshy berry-like cones helps to maintain the high infection rate and to disseminate mistletoe seeds at the distance of approximately 4 km. Modeling ecological niche and creating maps of potential range of the parasite and its principal host using MaxEnt 3.3.3k software have demonstrated that A. oxycedri distribution in Crimea at present may be wider than it has been currently observed. It is noteworthy that while modeling such bioclimatic indicators as the minimum winter temperatures and the elevation above sea level were irrelevant for establishing the distribution range of the parasite. Presumably the limited distribution of A. oxycedri can be attributed to the history of forming J. deltoides range in the late Pleistocene – Holocene, alongside with a low speed of the parasite dissemination from Quaternary refugia in the southernmost part of the Crimean Peninsula.

  4. Effects of insect and decapod exclusion and leaf litter species identity on breakdown rates in a tropical headwater stream

    Directory of Open Access Journals (Sweden)

    José Rincón

    2014-04-01

    Full Text Available La gran variedad de especies vegetales junto a los ríos tropicales afecta la diversidad de detritos orgánicos que llegan a los arroyos, creando variaciones temporales en cantidad y calidad de hojarasca. Examinamos la influencia de las especies vegetales y de la exclusión de macroinvertebrados sobre la descomposición de hojarasca en la parte alta de un arroyo en las montañas Luquillo de Puerto Rico. Incubamos hojarasca de Dacryodes excelsa (Burseraceae, Guarea guidonia (Meliaceae, Cecropia scheberiana (Moraceae, Manilkara bidentata (Sapotaceae y Prestoea acuminata (Palmae en bolsas descomposición que colocamos en una poza de Quebrada Prieta. Usamos bolsas de malla fina para excluir los macroinvertebrados y de malla gruesa para permitir la entrada a crustáceos decápodos (camarones y cangrejos jóvenes e insectos acuáticos (principalmente efemerópteros, quironómidos y tricópteros. D. excelsa y G. guidonia (en ambos tipos de malla tuvieron tasas de descomposición más altas que C. scheberiana, M. bidentata y P. acuminata. La descomposición fue más rápida para todas las especies de hoja con la malla gruesa, resaltando el papel de los descomponedores en este hábitat. Tras 42 días de esta incubación, las densidades totales de invertebrados, efemerópteros y tricópteros fueron mayores en las bolsas con hojas de D. excelsa y G. guidonia, e inferiores en P. acuminata, C. scheberiana y M. bidentata. A mayor densidad de insectos hubo mayor tasa de descomposición. Nuestros resultados indican la importancia de la especie de las hojas y de la presencia de macroinvertebrados en el proceso de descomposición de hojarasca en partes altas de arroyos tropicales.

  5. Penicillium excelsum sp. nov from the Brazil Nut Tree Ecosystem in the Amazon Basin’

    Science.gov (United States)

    Taniwaki, Marta Hiromi; Pitt, John I.; Iamanaka, Beatriz T.; Massi, Fernanda P.; Fungaro, Maria Helena P.; Frisvad, Jens C.

    2015-01-01

    A new Penicillium species, P. excelsum, is described here using morphological characters, extrolite and partial sequence data from the ITS, β-tubulin and calmodulin genes. It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest. The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences. The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype. PMID:26717519

  6. Determination of high molecular mass compounds from Amazonian plant's leaves; Determinacao de compostos de massa molecular alta em folhas de plantas da Amazonia

    Energy Technology Data Exchange (ETDEWEB)

    Siqueira, Denilson Soares de; Pereira, Alberto dos Santos; Aquino Neto, Francisco Radler de [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Quimica]. E-mail: ladetec@iq.gov.br; Cabral, Jose Augusto; Ferreira, Carlos Alberto Cid [Instituto Nacional de Pesquisas da Amazonia (INPA), Manaus, AM (Brazil); Simoneit, Bernd R.T. [Oregon State Univ., Corvallis, OR (United States). College of Oceanic and Atmospheric Sciences. Petroleum and Environmental Geochemistry Group; Elias, Vladimir O. [Analytical Solution, Rio de Janeiro, RJ (Brazil)

    2003-10-01

    The fractions of dichloromethane extracts of leaves from andiroba (Carapa guianensis - Meliaceae), caapi (Banisteriopsis caapi - Malpighiaceae), cocoa (Theobroma cacao - Sterculiaceae), Brazil nut (Bertholletia excelsa - Lecytidaceae), cupuacu (Theobroma grandiflorum - Sterculiaceae), marupa (Simaruba amara - Simaroubaceae) and rubber tree (Hevea brasiliensis - Euphorbiaceae), were analyzed by HT-HRGC and HT-HRGC-MS. Esters of homologous series of fatty acids and long chain alcohols, phytol, amyrines and tocopherols were characterized. The characterization of the compounds was based mainly in mass spectra data and in addition by usual spectrometric data ({sup 1}H and {sup 13}C NMR, IR). (author)

  7. Determination of high molecular mass compounds from Amazonian plant's leaves

    International Nuclear Information System (INIS)

    Siqueira, Denilson Soares de; Pereira, Alberto dos Santos; Aquino Neto, Francisco Radler de; Simoneit, Bernd R.T.

    2003-01-01

    The fractions of dichloromethane extracts of leaves from andiroba (Carapa guianensis - Meliaceae), caapi (Banisteriopsis caapi - Malpighiaceae), cocoa (Theobroma cacao - Sterculiaceae), Brazil nut (Bertholletia excelsa - Lecytidaceae), cupuacu (Theobroma grandiflorum - Sterculiaceae), marupa (Simaruba amara - Simaroubaceae) and rubber tree (Hevea brasiliensis - Euphorbiaceae), were analyzed by HT-HRGC and HT-HRGC-MS. Esters of homologous series of fatty acids and long chain alcohols, phytol, amyrines and tocopherols were characterized. The characterization of the compounds was based mainly in mass spectra data and in addition by usual spectrometric data ( 1 H and 13 C NMR, IR). (author)

  8. Comentarios médicos

    Directory of Open Access Journals (Sweden)

    Jorge Bejarano

    1941-08-01

    Full Text Available El ilustre varón, como todos los de su excelsa estirpe, que en hora trágica para la patria y para la medicina, acaba de perder la vida. Sólo elogios merece el proyecto de ley que sobre seguro social colectivo acaba de presentar a las cámaras el ministerio del trabajo. El brote epidémico del tifo exantemático, que con razón ha tenido tan alarmada a la ciudad nos sugiere dos enseñanzas.

  9. Planktonic foraminiferal biostratigraphy of the Upper Barremian and Aptian of Crimea

    Science.gov (United States)

    Brovina, E. A.

    2017-09-01

    Reexamination of the Barremian-Aptian planktonic foraminifers from three sections (Verkhoirechie, mountain Krasnaya, and Marino) allowed the biostratigraphic scheme for Southwest and Central Crimea to be refined and updated. The following standard zones are recognized in the studied sections: Blowiella blowi (upper Barremian), Hedbergella excelsa (upper Barremian-lower Aptian), Leupoldina cabri (lower Aptian), H. luterbacheri, Globigerinelloides ferreolensis, Gl. barri, Gl. algerianus, Hedbergella trocoidea, Paraticinella rohri (upper Aptian). Beds with Hedbergella ruka are recognized in the B. blowi Zone. Foraminifers from the Partizanskoe section, representing the lower Aptian L. cabri and H. luterbacheri zones, are studied. The recognized strata are correlated with ammonite and nannoplankton zones and paleomagnetic data.

  10. Penicillium excelsum sp. nov from the Brazil Nut Tree Ecosystem in the Amazon Basin'.

    Science.gov (United States)

    Taniwaki, Marta Hiromi; Pitt, John I; Iamanaka, Beatriz T; Massi, Fernanda P; Fungaro, Maria Helena P; Frisvad, Jens C

    2015-01-01

    A new Penicillium species, P. excelsum, is described here using morphological characters, extrolite and partial sequence data from the ITS, β-tubulin and calmodulin genes. It was isolated repeatedly using samples of nut shells and flowers from the brazil nut tree, Bertolletia excelsa, as well as bees and ants from the tree ecosystem in the Amazon rainforest. The species produces andrastin A, curvulic acid, penicillic acid and xanthoepocin, and has unique partial β-tubulin and calmodulin gene sequences. The holotype of P. excelsum is CCT 7772, while ITAL 7572 and IBT 31516 are cultures derived from the holotype.

  11. Chemical Compositions and Cytotoxic Activities of Leaf Essential Oils of Four Lauraceae Tree Species from Monteverde, Costa Rica

    OpenAIRE

    Maria C. Palazzo; Brittany R. Agius; Brenda S. Wright; William A. Haber; Debra M. Moriarity; William N. Setzer

    2009-01-01

    The leaf essential oils of four members of the Lauraceae Licaria excelsa, Licaria triandra, Perseaschiedeana, and Rhodostemonodaphne kunthiana, from Monteverde, Costa Rica, were obtained byhydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). The leaf oil of L. excelsawas dominated by the monoterpenes a-pinene (42.9%), b-pinene (22.0%) and myrcene (17.2%), while L.triandra was also rich in pinenes (40.9% and 28.5%, respectively). Persea schiedeana had considerableamo...

  12. ADDITIONAL NOTE ON CULLENIA CEYLANICA K. Schum. (Bombac)

    OpenAIRE

    A. J. G. H. KOSTERMANS

    2015-01-01

    Cullenia excelsa Wight in Pearson & Brown, Commerc. Timb. India 1:141, t. 51 1932; Foxworthy in Philipp. J. Sci. Bot. 4: 500. 1909; Trotter, Common Comm. Timb. India 65. 1901. Foxworthy in Philip. J. Sci. Bot. 4: 500. 1909.Fruit collected in January 1958 from the tree cultivated in the BogorBotanical Garden (no. XVI H. 13) enables me to correct and emendatethe description published in "Communication of the Forest ResearchInstitute, Bogor" no. 51 (1956) and in Reinwardtia 4 : 69.1956.The r...

  13. PABLO CARDOSO: UN GIRO ÉTICO Y POLÍTICO DE SU ESTÉTICA

    OpenAIRE

    Suárez Moreno, Cecilia

    2016-01-01

    PABLO CARDOSO: AN ETHICAL AND POLITICAL SPIN OF HIS AESTHETICResumen: Pablo Cardoso (Cuenca, 1965), artista contemporáneo cuya excelsa obra estuvo hasta ahora marcada por el escepticismo y una estética contemplativa, adopta un giro ético y político que, sin adherir a tesis alguna de ningún partido ni movimiento políticos, revela las contradicciones de la lógica del capital, particularmente del modelo extractivista, que contamina, agrede y destruye muchas formas de vida. Este giro ético y polí...

  14. As espécies de Coccoloba P. Browne (Polygonaceae) da Amazônia brasileira

    OpenAIRE

    Melo,Efigênia de

    2004-01-01

    O gênero Coccoloba está representado na Amazônia brasileira por 23 espécies: Coccoloba acuminata Kunth, C. arborescens (Vell.) R. A. Howard, C. ascendens Duss ex Lindau, C. brasiliensis Nees & Mart., C. charitostachya Standl., C. conduplicata Maguire, C. coronata Jacq., C. declinata (Vell.) Mart., C. densifrons Mart. ex Meisn., C. excelsa Benth., C. gentryi R. A. Howard, C. latifolia Lam., C. lehmannii Lindau, C. lucidula Benth., C. marginata Benth., C. mollis Casar., C. ovata Benth., C. ...

  15. The Species of Cocoloba P. Browne (Polygonaceae) from brasilian Amazonia

    OpenAIRE

    Melo, Efigênia de

    2004-01-01

    O gênero Coccoloba está representado na Amazônia brasileira por 23 espécies: Coccoloba acuminata Kunth, C. arborescens (Vell.) R. A. Howard, C. ascendens Duss ex Lindau, C. brasiliensis Nees & Mart., C. charitostachya Standl., C. conduplicata Maguire, C. coronata Jacq., C. declinata (Vell.) Mart., C. densifrons Mart. ex Meisn., C. excelsa Benth., C. gentryi R. A. Howard, C. latifolia Lam., C. lehmannii Lindau, C. lucidula Benth., C. marginata Benth., C. mollis Casar., C. ovata Benth., C. para...

  16. Determinação de compostos de massa molecular alta em folhas de plantas da Amazônia

    Directory of Open Access Journals (Sweden)

    Siqueira Denilson Soares de

    2003-01-01

    Full Text Available The fractIons of dichloromethane extracts of leaves from andiroba (Carapa guianensis - Meliaceae, caapi (Banisteriopsis caapi - Malpighiaceae, cocoa (Theobroma cacao - Sterculiaceae, Brazil nut (Bertholletia excelsa - Lecytidaceae, cupuaçu (Theobroma grandiflorum - Sterculiaceae, marupá (Simaruba amara - Simaroubaceae and rubber tree (Hevea brasiliensis - Euphorbiaceae, were analyzed by HT-HRGC and HT-HRGC-MS. Esters of homologous series of fatty acids and long chain alcohols, phytol, amyrines and tocopherols were characterized. The characterization of the compounds was based mainly in mass spectra data and in addition by usual spectrometric data (¹H and 13C NMR, IR.

  17. Obtención de pilocarpina a partir de Pilocarpus racemosus Vahl.

    Directory of Open Access Journals (Sweden)

    Armando Payo Hill

    1995-08-01

    Full Text Available Se inicia el trabajo fitoquímico en Pilocarpus racemosus Vahl. (Rutaceae con la finalidad de conocer el contenido de pilocarpina en esta especie. Se extrajeron 1,4 kg de tallos y 1 kg de hojas por los métodos convencionales, y se cromatografiaron los crudos alcaloidales en columnas, de las cuales se obtuvo 2,3036 y 0,6871 g de pilocarpina pura respectivamente. La identificación del alcaloide se realizó por espectroscopia infrarroja contra patrón y cromatografía de capa fina.

  18. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) in Brazil.

    Science.gov (United States)

    Marsaro Júnior, A L; Peronti, A L B G; Costa, V A; Morais, E G F; Pereira, P R V S

    2016-02-01

    Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam.) de Wit (Fabaceae), Morus nigra L. (Moraceae), Citrus reticulata Blanco (Rutaceae), Tectona grandis L. f. (Verbenaceae), Anacardium occidentale (Anacardiaceae), Annona squamosa L. and Xylopia aromatica (Lam.) Mart. (Annonaceae), in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  19. Toxicity of a furanocoumarin to armyworms: a case of biosynthetic escape from insect herbivores.

    Science.gov (United States)

    Berenbaum, M

    1978-08-11

    When the linear furanocoumarin xanthotoxin, found in many plants of the families Rutaceae and Umbelliferae, was administered to larvae of Spodoptera eridania, a generalist insect herbivore, it displayed toxic properties lacking in its biosynthetic precursor umbelliferone. Reduced toxicity observed in the absence of ultraviolet light is consistent with the known mechanism of photoinactivation of DNA by furanocoumarins through ultraviolet-catalyzed cross-linkage of strands. Thus, the ability of a plant to convert umbelliferone to linear furanocoumarins appears to confer broader protection against insect herbivores.

  20. CONSTITUYENTES QUIMICOS DE Zanthoxylum monophyllum

    Directory of Open Access Journals (Sweden)

    Luis E. Cuca S

    2010-10-01

    Full Text Available De los extractos etanólicos de hojas, corteza y madera de Zanthoxylum monophyllum (Rutaceae se aislaron e identificaron cinco lignanos: sesamina, eudesmina, asarinina, hinokinina y cubebina (mezcla de epímeros; dos alcaloides: y-fagarina y skimmianina; dos terpenos: sltosterol y lupeol; un ácido graso y un éster: ácido oleico y linoleato de etilo; dos acetofenonas: xanthoxylina y 2,4-dihidroxi-6-metoxiacetofenona; tres coumarinas: osthenol, arnottinina y columbianetina. Sus estructuras fueron determinadas por análisis espectroscópico, comparación con muestras auténticas y datos reportados en la literatura.

  1. Methylenedioxy- and methoxyflavones from Melicope coodeana syn. Euodia simplex

    DEFF Research Database (Denmark)

    Simonsen, Henrik Toft; Larsen, Michael D; Nielsen, Merete W

    2002-01-01

    Three new natural products, 3,8-dimethoxy-5,7-dihydroxy-3',4'-methylenedioxyflavone, 3,6,8-trimethoxy-5,7-dihydroxy-3',4'-methylenedioxyflavone and 3,6,8,3',4'-pentamethoxy-5,7-dihydroxyflavone were isolated from Melicope coodeana syn. Euodia simplex (Rutaceae) along with 3,6,3'-trimethoxy-5......,7,4'-trihydroxyflavone and 3,3'-dimethoxy-5,7,4'-trihydroxyflavone. The structural assignments are based on (1)H and (13)C NMR data, including discussion of the chemical shifts of C-2 in 3,5-dihydroxy- and 3-methoxy-5-hydroxyflavones. The presence of highly methoxylated and methylenedioxyflavones is characteristic...

  2. Avaliação de furanocumarinas como inibidores da fotossíntese através de ensaios de fluorescência da clorofila a

    Directory of Open Access Journals (Sweden)

    Olívia Moreira Sampaio

    2012-01-01

    Full Text Available The evaluations of Chorophyll a fluorescence emitted by superior plants carry structural information and photosynthetic apparatus function. Quantitative analysis apparatus of fluorescence kinetic were measured by energy flows (ABS, (TR, (ET and (DI, known as phenomenological phenomena of OJIP test. Four furocoumarins were isolated from Ruta graveolens (Rutaceae, and chorophyll a (Chl a fluorescence assays were performed with these compounds to evaluate the photosynthesis inhibition potential. This test was realized in spinach`s leaf discs and in Lolium perenne leaves. The results indicated the herbicide potential mainly for bergapten and chalepin.

  3. Extraction, Modelling and Purification of Flavonoids from Citrus Medica Peel

    OpenAIRE

    M. Parvathi Nandan; Vangalapati Meena

    2015-01-01

    Soxhlet extraction technique is widely employed for the extraction and separation of chemical constituents in the medicinal plants. Citrus medica L commonly called as Citron belongs to family Rutaceae, is a slow-growing shrub. It is mainly cultivated for the production of edible fruits which are sour in taste like lime and lemon and the main content of a citron fruit is the thick rind, which is very adherent to the segments. From the phytochemical analysis the peel extract is rich source of p...

  4. Drug: D06689 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D06689 Crude ... Drug Phellodendron bark (JP17); Powdered phellodendron bark (JP17); Phello...dendron bark (TN) Berberine [CPD:C00757], Palmatine [CPD:C05315], Magnoflorine [CPD:C09581], Phello...esterol [CPD:C01789], Guanidine [CPD:C17349], Candicine [CPD:C10575], Menisperine, 7-Dehydrostigmasterol ... Phello...dendron amurense [TAX:68554], Phellodendron chinense [TAX:354508] ... Same as: E00063 Therapeutic catego...ry: 5100 ... Rutaceae (rue family) Phellodendron bark Major component: Berberine [CPD:C00757] ... PubChem: 47208340 ...

  5. Isolation and Structural Elucidation of an Unknown compound from Murraya alternans (Kurz)Swingle

    International Nuclear Information System (INIS)

    Mya Aye; Hla Myoe Min; Sein Htun

    2002-02-01

    A new taxon of a species, Murraya alternans (Kurz) Swingle (Myanmar name, Naganaing) the series of Murraya belonging to the family Rutaceae had been recognized by Peter G. Waterman in 1986. However, this species has not been undertaken in botanical, medical, and chemical aspects. In this paper, scientific study on this taxon was chemically carried out for the first time. One of the unknown compounds was isolated from this species by column and high performance liquid chromatographic methods. It's partial structure could also be elucidated by spectral analysis such as IR, MS, H NMR(400MHz), C NMR (100MHz) spectrometry respectively. (author)

  6. Evaluation of fruit extracts of six Turkish Juniperus species for their antioxidant, anticholinesterase and antimicrobial activities.

    Science.gov (United States)

    Oztürk, Mehmet; Tümen, İbrahim; Uğur, Aysel; Aydoğmuş-Öztürk, Fatma; Topçu, Gülaçtı

    2011-03-30

    Juniperus L. (Cupressaceae) species are mostly spread out in the Northern Hemisphere of the world, and some of them are used as folkloric medicines. The fruits of some species are eaten. Since oxidative stress is one of the reasons for neurodegeneration and is associated with the Alzheimer's disease (AD), the extracts prepared from the fruits of six Juniperus species were screened for their antioxidant activity. Therefore, the extracts were also evaluated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), which are chief enzymes in the pathogenesis of AD. In addition, antimicrobial activity was also evaluated. In the β-carotene-linoleic acid assay, acetone extracts of J. oxycedrus subsp. oxycedrus, J. sabina and J. excelsa, and methanol extracts of J. phoenicea and J. sabina, effectively inhibited oxidation of linoleic acid. The hexane extracts of J. oxycedrus subsp. oxycedrus, J. foetidissima and J. phoenicea showed remarkable inhibitory effect against AChE and BChE. Because of their high antioxidant activity, J. excelsa, J. oxycedrus subsp. oxycedrus, J. sabina and J. phoenicia might be used in the food industry as preservative agents or extension of the shelf-life of raw and processed foods. Since the hexane extracts of J. oxycedrus subsp. oxycedrus and J. foetidissima demonstrated significant anticholinesterase activity they should be considered as a potential source for anticholinesterase agents. Copyright © 2010 Society of Chemical Industry.

  7. Identité et écologie des espèces forestières commerciales d'Afrique centrale : le cas de Milicia spp. (synthèse bibliographique

    Directory of Open Access Journals (Sweden)

    Daïnou, K.

    2012-01-01

    Full Text Available Identity and ecology of Central African timber tree species: the case of Milicia spp., a review. Iroko designates the two African tree species Milicia excelsa (Welw. C.C.Berg and Milicia regia (A.Chev. C.C.Berg known as highly valuable in the timber trade. Despite its economical importance and a decrease in population densities due to exploitation, there is a significant lack of ecological and sylvicultural knowledge required for a sustainable management of iroko. In West Africa, M. excelsa has received more attention from scientists than M. regia, but several topics clearly need further investigations or outcomes cannot be transferred in the context of Central African rainforests. A first thing to verify is whether speciation really occurred in the genus Milicia: morphological differences between the two species are very low, and a revision in this genus may lead to some rearrangements of conservatory and management policies related to this taxonomic group. Furthermore, (i patterns related to the reproductive phenology and its controlling factors, (ii gene flow among and between populations and individuals regarding life history traits, influence of human activities and paleoecological perturbations, and (iii dynamics of seedling populations are among aspects that require emphasis in order to propose efficient tools for forest managers. Unless scientists provide these crucial data, the abundance of iroko in tropical rainforests will only depend on expensive plantations activities.

  8. Phytochemical analysis and cytotoxicity towards multidrug-resistant leukemia cells of essential oils derived from Lebanese medicinal plants.

    Science.gov (United States)

    Saab, Antoine M; Guerrini, Alessandra; Sacchetti, Gianni; Maietti, Silvia; Zeino, Maʼen; Arend, Joachim; Gambari, Roberto; Bernardi, Francesco; Efferth, Thomas

    2012-12-01

    Juniperus excelsa fruit essential oil as well as J. oxycedrus, Cedrus libani, and Pinus pinea wood essential oils have been obtained with yields between 2.2 ± 0.3 % to 3.4 ± 0.5 % and analyzed by gas chromatography. Sesquiterpenes mainly characterized C. libani and J. oxycedrus essential oils, while in P. pinea and J. excelsa, monoterpenes were the most abundant compounds. In J. oxycedrus, cis-calamenene (7.8 %), cuparene (3.8 %), and cis-thujopsenal (2.0 %) have been detected for the first time. The cytotoxic activity of these essential oils against drug-sensitive CCRF-CEM and multidrug-resistant P-glycoprotein-expressing CEM/ADR5000 leukemia cells has been investigated (IC₅₀ values: 29.46 to 61.54 µg/mL). Remarkably, multidrug-resistant CEM/ADR5000 cells did not reveal cross-resistance, indicating that these essential oils might be useful to treat otherwise drug-resistant and refractory tumors. Georg Thieme Verlag KG Stuttgart · New York.

  9. Origen botánico y geográfico de las mieles de El Fuerte, Departamento de Santa Bárbara, Jujuy, Argentina Botanical and geographical origin of honeys from El Fuerte, Department of Santa Bárbara, Jujuy, Argentina

    Directory of Open Access Journals (Sweden)

    Ana C. Sánchez

    2011-06-01

    Full Text Available Este es el primer aporte al conocimiento del origen botánico y geográfico de las mieles de la Provincia de Jujuy. Se analizaron veinticuatro muestras procedentes de la Localidad El Fuerte Departamento Santa Bárbara, reconociéndose un total de cincuenta tipos polínicos pertenecientes a treinta y dos familias botánicas. Se registraron 33% de mieles Uniflorales y 67% de mieles Multiflorales. Se destacan los siguientes tipos polínicos: Gleditsia amorphoides, Myrtaceae y Scutia-Condalia acompañados por Allophylus edulis, Parapiptadenia excelsa, Schinus sp. y Zanthoxylum coco. El espectro polínico obtenido caracteriza geográficamente estas mieles, reflejando la vegetación nativa del área de estudio: ecotono entre la Selva Montana de las Yungas y el Chaco Serrano.This is the first contribution to the knowledge of botanical and geographical origin of honey from the Province of Jujuy. Twenty-four samples were analyzed from the locality of El Fuerte, Santa Bárbara Department. Fifty pollen types were identified belonging to thirty-two botanical families, were registering a 33% honey unifloral and 67% multifloral honey. Include the following pollen types: Gleditsia amorphoides, Myrtaceae and Scutia-Condalia, accompanied by Allophylus edulis, Parapiptadenia excelsa, Schinus sp y Zanthoxylum coco. The pollen spectrum obtained characterized these honeys and reflects the native vegetation: ecotone between the Selva Montana of the Yungas and Chaco Serrano.

  10. Crescimento, fotossíntese e composição mineral em genótipos de Coffea com potencial para utilização como porta-enxerto Growth, photosynthesis and mineral composition of Coffea germplasm with potential as rootstock

    Directory of Open Access Journals (Sweden)

    Eduardo Lauriano Alfonsi

    2005-01-01

    Full Text Available Foi estudado o desenvolvimento da parte aérea e das raízes, as trocas gasosas fotossintéticas e a composição mineral nos genótipos de Coffea, Apoatã IAC 2258 (C. canephora; Bangelan IAC col. 5 (C. congensis X C. canephora; Catuaí IAC 144 (C. arabica; Excelsa (C. liberica, var. dewevrei e Piatã (IAC 387 (C. arabica X C. liberica, var. dewevrei, visando conhecer seus potenciais para utilização como porta-enxerto em C. arabica. Utilizaram-se plantas jovens cultivadas em terra, contida em saco plástico e em tubos de PVC sob telado, e no campo em plantas de quatro anos de idade. Em 'Bangelan' observaram-se maior comprimento da raiz pivotante e mais quantidade de raízes secundárias do que em 'Catuaí' e 'Piatã'. Os teores foliares de potássio do 'Piatã', 'Apoatã' e 'Bangelan' foram maiores aos observados para 'Catuaí' e 'Excelsa'. No campo, com baixa disponibilidade de água e nutrientes, os teores foliares de fósforo em 'Apoatã' e 'Piatã' foram maiores que em 'Catuaí' e 'Excelsa'. Em 'Catuaí,' notou-se maior eficiência na absorção de todos os micronutrientes (B, Cu, Mn e Zn, exceto ferro. A taxa fotossintética, condutância estomática e transpiração decresceram à tarde em todos os genótipos. Sob défice hídrico no solo, houve quedas significativas nas taxas fotossintéticas, condutância estomática e transpiração, sendo mais pronunciadas em 'Apoatã' e 'Excelsa', e menos em 'Catuaí' e 'Piatã', em relação aos valores observados sem restrição hídrica. A taxa fotossintética do 'Apoatã' foi menos influenciada pelo teor de água no solo em relação aos demais genótipos, que mostraram quedas acentuadas no período da tarde. Verificaram-se em 'Bangelan' e 'Apoatã' características favoráveis para a utilização como porta-enxerto em C. arabica.The development of shoot and roots, photosynthetic gas exchange and mineral composition of Coffea plants were studied in 'Apoatã' IAC 2258 (C. canephora, 'Bangelan

  11. Economically important species dominate aboveground carbon storage in forests of southwestern Amazonia

    Directory of Open Access Journals (Sweden)

    N. Galia Selaya

    2017-06-01

    Full Text Available Tree species in tropical forests provide economically important goods and ecosystem services. In submontane forests of southwestern Amazonia, we investigated the degree to which tree species important for subsistence and trade contribute to aboveground carbon storage (AGC. We used 41 1-hectare plots to determine the species abundance, basal area, and AGC of stems > 10 cm diameter at breast height (dbh. Economically important taxa were classified using ethnobotanical studies and according to their stem density. These taxa (n = 263 accounted for 45% of total stems, 53% of total basal area, and 56% of total AGC, significantly more than taxa with minor or unknown uses (Welch test at p 40 cm and few stems in regeneration classes of dbh < 10 to 20 cm (e.g., Bertholletia excelsa, Cariniana spp., Cedrelinga spp., Ceiba spp., Dipteryx spp., whereas dominant Tetragastris spp., and Pseudolmedia spp. had most stems in low diameter classes and a median diameter of < 30 cm. Bertholletia excelsa, with 1.5 stems per hectare, showed the highest basal area of any species and accounted for 9% of AGC (11 Mg/ha, twice that of the second-ranking species. Our study shows that economic importance and carbon stocks in trees are closely linked in southwestern Amazonia. Unplanned harvests can disrupt synergistic dual roles altering carbon stocks temporally or permanently. Precautionary measures based on species ecology, demography, and regeneration traits should be at the forefront of REDD+ to reconcile maximum harvesting limits, biodiversity conservation, and sustainable forest management.

  12. Absorption of some mineral salts by root system of different woody species and accumulation over a whole vegetative cycle (1963); Absorption de quelques sels par l'appareil radiculaire de differentes especes ligneuses et accumulation au cours d'un cycle vegetatif complet (1963)

    Energy Technology Data Exchange (ETDEWEB)

    Gagnaire, J; Gerard, J M [Commisariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1963-07-01

    The concentration power of plant tissues and the translocation speed of mineral salts are considerably varying with the absorbed salt, the botanical species, the considered tissue and the part of the vegetative cycle. In Grenoble, with Picea excelsa, the 'true dormancy' is short (half-november, end of december). It is accompanied by a pre-dormancy period (October, half-november) and a post dormancy period (January, february, march). In vegetative period, Picea excelsa leaves are less concentrating mineral salt than Acer campestris leaves (coefficient 2 for calcium - 3 for phosphates) and Populus nigra leaves (coefficient 3 for calcium, coefficient 5 for phosphates). (author) [French] Le pouvoir de concentration de tissus vegetaux, et particulierement la vitesse de transport des sels mineraux varient beaucoup selon le sel mineral absorbe, l'espece vegetale, le tissu considere ainsi que la periode du cycle vegetatif. A Grenoble, pour les epiceas 'la dormance veritable' est courte (mi-novembre, fin decembre). Elle est encadree par des periodes de pre-dormance (octobre, mi-novembre) et de post-dormance (janvier, fevrier, mars). Pendant la periode vegetative, le pouvoir de concentration de sels mineraux des aiguilles d'epiceas est plus faible que celui des feuilles d'erables (coefficient 2 pour le calcium - coefficient 3 pour les phosphates) ou que celui des feuilles de peupliers (coefficient 3 pour le calcium - coefficient 5 pour les phosphates). (auteur)

  13. Mitigation of mercury contamination through the acceleration of vegetation succession

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    WIWIK EKYASTUTI

    2016-06-01

    Full Text Available Abstract. Ekyastuti W, Faridah E, Sumardi, Setiadi Y. 2016. Mitigation of mercury contamination through the acceleration of vegetation succession. Biodiversitas 17: 84-89. The success of the restoration of the tailings ex-gold mining through the succession is highly dependent on the ability of plants to grow and adapt to the troubled land. Restoration through natural succession takes a very long time. Therefore, human intervention is required to accelerate the succession. The purpose of this research was to improve the effectiveness of mitigation of mercury contamination through the acceleration of vegetation succession. This research has been carried out in a greenhouse using an experiment with a completely randomized design. There are 8 treatment consists of four indigenous species (Dillenia excelsa, Melastoma affine, Cinnamomum porrectum and Casuarina junghuhniana grown alone (one species and collective (more than one species in the tailing media with a mercury content of 20 ppm. The results showed that the planting collectively have a mutually supportive interaction, so that increased the plant growth. In addition, collective planting two or four different species of plants, and the D. excelsa itself could decrease the concentration of mercury in the tailing. The acceleration of vegetation succession through the right choice of plants species and planting collectively, capable to increasing the potential of mitigation of mercury contamination in the tailings.

  14. ETHNO-VETERINARY MEDICINAL USAGE OF FLORA OF GREATER CHOLISTAN DESERT (PAKISTAN

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    FRAZ M. KHAN

    2009-05-01

    Full Text Available A study on the ethno-veterinary usage of wild medicinal plants of Greater Cholistan desert of Pakistan was conducted from January, 2007 to December, 2008. Information regarding 35 plant species was collected. According to the results, Blepharis sindica was used as galactagogue. Butea monosperma, Calotropis procera and Phyllanthus nirurii were used as emollient, demulcent and antiphlogistic. Amaranthus trilocular, Capparis decidua, Clerodendron phlomoides, Phyllanthus nirurii and Ricinus communis were used as carminative and stomachic. Capparis decidua and Calotropis procera were used as appetizer. Prosopis glandulosa had anodyne properties, Achyranthes aspera had antilithic, while Pedalium murex, Tribulus terrestris and Barleria prionites had diuretic value. Achyranthes aspera, Argemone mexicana, Balanites aegyptiaca, Butea monosperma, Cassia senna, Citrullus colocynthis and Vitex negundo were used as vermifuge. Alhagi camelorum and Balanites aegyptiaca had aperient properties. Barleria prionites and Mollugo nudicaulis had their role in the ripening of an abscess. Ricinus communis and Salvadora oleoides aided in the removal of placenta and lochia. Anamitra cocculus and Argemone mexicana were used as febrifuge. Aerva javanica, Ailanthus excelsa, Amaranthus trilocular, Capparis decidua were used in diarrhoea and dysentery. Argemone mexicana and Ailanthus excelsa were used in ague.

  15. A new species of Neosilba (Diptera, Lonchaeidae from Brazil Uma nova espécie de Neosilba (Diptera, Lonchaeidae do Brasil

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    Pedro C. Strikis

    2009-09-01

    Full Text Available A new species of Neosilba McAlpine, 1962, N. pradoi sp. nov., is described and illustrated. This new species was found in the south of Brazil (Rio Grande do Sul and Santa Catarina, in the southeast (State of São Paulo and center west (State of Mato Grosso do Sul. It has been reared from fruits of guava (Psidium guajava, Myrtaceae, "araçá" (Psidium cattleyanum, Myrtaceae, "guabiroba" (Campomanesia xanthocarpa, Myrtaceae, Surinam cherry (Malpighia emarginata, Malpighiaceae, cherry (Prunus avium, Rosaceae, orange (Citrus sinensis, Rutaceae, "ingá" (Inga laurina, Fabaceae, "esporão-de-galo" (Celtis iguanae, Ulmaceae and passion fruit (Passiflora edulis, Passifloraceae.Uma nova espécie de Neosilba McAlpine, 1962, N. pradoi sp. nov., é descrita e ilustrada. Esta nova espécie foi encontrada no sul do Brasil (Rio Grande do Sul e Santa Catarina, no sudeste (Estado de São Paulo e na região centro-oeste (Estado do Mato Grosso do Sul. Foi obtida de frutos de goiaba (Psidium guajava, Myrtaceae, araçá (Psidium cattleyanum, Myrtaceae, guabiroba (Campomanesia xanthocarpa, Myrtaceae, acerola (Malpighia emarginata, Malpighiaceae, cereja (Prunus avium, Rosaceae, laranja (Citrus sinensis, Rutaceae, ingá (Inga laurina, Fabaceae, esporão-de-galo (Celtis iguanae, Ulmaceae e maracujá (Passiflora edulis, Passifloraceae.

  16. Effects of temperature and food on the development of Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae)

    International Nuclear Information System (INIS)

    Almeida, Fabio Souto; Goncalves, Lenicio

    2007-01-01

    Dysdercus maurus Distant, 1901 (Hemiptera, Pyrrhocoridae) is an important pest on Gossypium spp. (cotton tree), Citrus sinensis Osbeck (Rutaceae) and Citrus reticulata Blanco (Rutaceae) crops. This insect also feeds on seeds of Chorisia speciosa St. Hil. (Bombacaceae). This work aimed to evaluate the effects of temperature and food on the development of D. maurus. Eight treatments were carried out, in six of them bugs were fed with seeds of C. speciosa and kept at 15, 18, 20, 25 and 30 ± 1 deg C, 80 ± 3% RH and 12h photo phase or in laboratory conditions (23.5 ± 2.6 deg C, 73.3 ± 9.9 % RH), and in the other two treatments bugs were fed with seeds of cotton variety IAC-22 and kept at 25 or 30 deg C. In all treatments five immature stages were observed. The increase of temperature caused reduction in the developmental time. The temperature of 15 deg C disabled nymphal eclosion and was also lethal to those nymphs ecloded at other temperatures. The lower mortality of nymphs occurred in the temperature of 25 deg C with cotton as food (24.07%). The lower threshold temperature (Tb) occurred for the first instar (11.54 deg C) and the higher for the second instar (15.33 deg C). The females of D. maurus required more degree-days (329.93 degree-days) than males (300.49 degree-days) until adult emergence. (author)

  17. Effects of temperature and food on the development of Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae); Efeitos da temperatura e do alimento no desenvolvimento de Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae)

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Fabio Souto [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Dept. de Ciencias Ambientais], e-mail: fbio_almeida@yahoo.com.br; Goncalves, Lenicio [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Dept. de Biologia Animal. Area de Biologia], e-mail: lencygon@globo.com

    2007-10-15

    Dysdercus maurus Distant, 1901 (Hemiptera, Pyrrhocoridae) is an important pest on Gossypium spp. (cotton tree), Citrus sinensis Osbeck (Rutaceae) and Citrus reticulata Blanco (Rutaceae) crops. This insect also feeds on seeds of Chorisia speciosa St. Hil. (Bombacaceae). This work aimed to evaluate the effects of temperature and food on the development of D. maurus. Eight treatments were carried out, in six of them bugs were fed with seeds of C. speciosa and kept at 15, 18, 20, 25 and 30 {+-} 1 deg C, 80 {+-} 3% RH and 12h photo phase or in laboratory conditions (23.5 {+-} 2.6 deg C, 73.3 {+-} 9.9 % RH), and in the other two treatments bugs were fed with seeds of cotton variety IAC-22 and kept at 25 or 30 deg C. In all treatments five immature stages were observed. The increase of temperature caused reduction in the developmental time. The temperature of 15 deg C disabled nymphal eclosion and was also lethal to those nymphs ecloded at other temperatures. The lower mortality of nymphs occurred in the temperature of 25 deg C with cotton as food (24.07%). The lower threshold temperature (Tb) occurred for the first instar (11.54 deg C) and the higher for the second instar (15.33 deg C). The females of D. maurus required more degree-days (329.93 degree-days) than males (300.49 degree-days) until adult emergence. (author)

  18. Usos en medicina folclórica, actividad biológica y fitoquímica de metabolitos secundarios de algunas especies del género Zanthoxylum

    Directory of Open Access Journals (Sweden)

    Víctor Enríque Macias Villamizar

    2013-10-01

    Full Text Available ResumenSegún el Herbario Nacional de Colombia de la familia Rutaceae en Colombia se encuentran 150 géneros y 900 especies (1, de las cuales muchas han sido utilizada no sólo en la medicina folklórica (2, 3, 4, 5 en tratamientos digestivos, tónico estomático, diuréticos, sedativos entre otros; sino también determinada su actividad biológica como antiplasmódico y citotóxico (5. En la familia Rutaceae se encuentra el género Zanthoxylum (6, del cual también se utiliza en medicina folklórica que incluyen tratamientos contra la tos, enteritis, diarrea, resfriado, reumatismo y ulceraciones (7, 8, 9, 10, 11; también se ha ensayado su actividad biológica donde se encontró ser efectiva, entre otras, a nivel: antimicrobial (7, 8, 9; citotóxica (12,13; Antiagregación plaquetaria (14, 15, y antitumoral (16. La amplia gama de usos etnobotánicos y farmacológicos convierte a los extractos (o compuestos aislados del género Zanthoxylum en materia prima a utilizar en el desarrollo de ensayos para evaluar la actividad biológica, la cual está asociada a la riqueza de metabolitos secundarios tales como alcaloides, lignanos, terpenos, flavonoides, cumarinas, entre otros; convirtiendo al género Zanthoxylum en objeto de estudió fitoquímico promisorio.En este artículo se realiza un aporte al estudió del género Zanthoxylum, en aspectos relacionados con su fitoquímica, sus usos en medicina folclórica y su actividad biológica; y en consecuencia un aporte pertinente al conocimiento en la Facultad de Ciencias de la Salud, debido a la actual tendencia de recurrir a medicina natural para el desarrollo de medicamentos de relativo bajo costo y con mayor probabilidad de adquisición del mismo. (Duazary 2007; 2: 140 - 159AbstractAccording to the Herbarium National of Colombia the Rutaceae family in Colombia are 150 genus and 900 species (1, of which many have been used not only in the folkloric medicine (2, 3, 4, 5 in digestive treatments, stomatic

  19. Impact of grazing abandonment on floristic diversity in the priority habitat type *9562 Grecian Juniper Woods

    Science.gov (United States)

    Vrahnakis, Michael; Kazoglou, Yannis; Fotiadis, George; Kakouros, Petros; Nasiakou, Stamatia; Soutsas, Konstantinos

    2017-04-01

    The habitat type *9562 Grecian juniper woods (Juniperetum excelsae) includes Greek juniper (Juniperus excelsa M. Bieb.) forests and they are found mainly in the western sector of the Prespa National Park, NW Greece. Greek juniper forests are considered extremely rare for EU-28, recommending a priority habitat type in accordance with Directive 92/43/EEC. In addition, their ecological importance is great given its high plant taxa richness; they harbor most of the 900 plant taxa found in the western sector of the Park, many of them being important for EU or global scale. The accelerated invasion of deciduous hardwoods is the most significant risk for the habitat, since its rich flora is well-adapted to open light conditions produced by the open spaced Greek junipers. Also, the dense vegetated conditions deprive the regeneration of the photophilous Greek juniper. The invasion results from the lack of its natural controller, i.e. the grazing livestock. It is estimated that the total area of juniper forests for the Devas area decreased to 89% of the area of 1945 in favor of invasive hardwoods. The paper presents the analysis of the floristic diversity of the priority habitat type *9562 Grecian Juniper Woods (Juniperetum excelsae) (GJWs). Four (4) types of juniper forest ranges (GJWs) were distinguished in terms of canopy cover: (a) pure GJWs, (b) mixed open GJWs, (c) open GJWs, and (d) mixed dense GJWs. A total of 171 plant taxa were recorded, and distributed within 43 botanical families; the largest one being Leguminosae (26 taxa). The statistically estimated plant taxa richness for pure GJWs was 116.4, for mixed open 152.6, for open 57.9, and for mixed dense 90.2 taxa. The analysis of α-diversity indices did not reveal any specific trend of diversity for the four GJWs. The behavior of the variability of diversity among the four range types of GJWs was depending on the emphasis the used indices place on properties such as taxa richness or abundance. This fact was

  20. Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2

    Energy Technology Data Exchange (ETDEWEB)

    Guo, Feng; Jin, Tengchuan; Howard, Andrew; Zhang, Yu-Zhu, E-mail: zhangy@iit.edu [Department of Biology, Illinois Institute of Technology, Chicago, IL 60616 (United States)

    2007-11-01

    The crystallization of the brazil nut allergen Ber e 2 is reported. Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study, Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were obtained using the hanging-drop vapour-diffusion method. A molecular-replacement structural solution has been obtained. Refinement of the structure is currently under way.

  1. Análise da estrutura de comunidades arbóreas de uma floresta amazônica de Terra Firme aplicada ao manejo florestal Analysis of the structure of tree communities of a amazonian forest applied to management

    Directory of Open Access Journals (Sweden)

    João Carlos Zenaide Oliveira Alves

    2008-12-01

    Full Text Available Este trabalho teve o objetivo de identificar e descrever a estrutura das comunidades arbóreas de uma floresta de terra firme sob regime de manejo na Amazônia Oriental, localizada no município de Almeirim, Pará, Brasil. O levantamento florístico foi realizado em 1.400,30 ha de uma Unidade de Produção Anual (UPA, do Plano de Manejo Florestal da empresa ORSA Florestal. Todos os indivíduos com DAP > 30 cm foram inventariados, registrando-se 77.834 árvores distribuídas em 57 famílias, 229 gêneros e 556 espécies. Oito comunidades foram identificadas por meio de uma analise de agrupamento, apresentando alta diversidade e equibilidade florística (H" médio = 4,25 e J" médio = 0,75. As comunidades apresentaram 138 espécies comuns, 119 espécies de ocorrência exclusiva e 377 espécies raras, representadas por apenas um indivíduo. As espécies que mais se destacaram foram: Dinizia excelsa, Vouacapoua americana, Goupia glabra, Mouriri brachyanthera, Parinari excelsa, Manilkara bidentada, Tachigalia mymecophyla e Licania micrantha. Algumas espécies de valor comercial apresentaram variações importantes na densidade, sugerindo risco de extinção em comunidades onde as espécies apresentam densidade muito baixa. Sugerimos que os planos de manejo considerem as várias comunidades ecológicas encontradas nas UPAs evitando assim variações significativas, causadas pela exploração e seus impactos, na composição florística e estrutura das comunidades existentes.To demonstrate the importance of the ecological concept for forest management, the object of this work was to identify and describe the structure of tree communities of a tropical forest under management in the Eastern Amazonia, located in Almeirim municipality, in the state of Pará, Brazil. The floristic survey was undertaken in a terra-firme tropical forest of 1,400.30 hectares. All individuals with DBH > 30 cm were inventoried, registering 77,834 trees distributed in 57

  2. Los Pereira de Castro Montenegro de Arcos en Tui

    Directory of Open Access Journals (Sweden)

    Iglesias Almeida, Ernesto

    2000-12-01

    Full Text Available Not avalaible

    Esta antigua familia de origen portugués constituyó una de las más ilustres de la nobleza tudense de finales del siglo XVI. Magníficamente estudiada por Valdés Costas', quisiéramos en este trabajo evocar sus más excelsas glorias gracias a los interesantes datos que nos suministra la escritura de institución del vínculo y mayorazgo realizada por el Licenciado Paulo Pereira de Castro de Sousa y su esposa doña María de Montenegro en 1608 en favor de su única heredera, su hija doña María Pereira de Castro y Montenegro. Documento varias veces citado por los investigadores pero que había permanecido inédito hasta el presente.

  3. Floristic and phytosociology in dense "terra firme" rainforest in the Belo Monte Hydroelectric Plant influence area, Pará, Brazil.

    Science.gov (United States)

    Lemos, D A N; Ferreira, B G A; Siqueira, J D P; Oliveira, M M; Ferreira, A M

    2015-08-01

    The objective of the present study was to characterise the floristic and phytosociological composition on a stretch of dense "Terra Firme" rainforest located in the Belo Monte hydroelectric plant area of influence, located in the state of Pará, Brazil. All trees with DAP >10 cm situated in 75 permanent plots of 1 ha were inventoried. 27,126 individuals trees (361 ind.ha-1), distributed in 59 botanical families, comprising 481 species were observed. The families with the largest number of species were Fabaceae (94), Araceae (65) and Arecaceae (43), comprising 43.7% of total species. The species Alexa grandiflora (4.41), Cenostigma tocantinum (2.50) and Bertholletia excelsa (2.28) showed the highest importance values (IV). The ten species with greater IV are concentrated (22%). The forest community has high species richness and can be classified as diverse age trees, heterogeneous and of medium conservation condition.

  4. Antisecretory activity of plants used to treat gastrointestinal disorders in Mexico.

    Science.gov (United States)

    Velázquez, Claudia; Calzada, Fernando; Torres, Javier; González, Felipe; Ceballos, Guillermo

    2006-01-03

    Aqueous and methanolic extracts from 26 medicinal plants used in Mexico to treat gastrointestinal disorders were screened to evaluate their antisecretory activity on cholera toxin-induced intestinal secretion in rat jejunal loops model. Extracts were tested at a dose of 300 mg/kg. From 56 samples tested, both extracts from Chiranthodendron pentadactylon, Hippocratea excelsa and Ocimum basilicum were the most potent with inhibition values ranging from 68.0 to 87.6%. On the other hand, the methanolic extract of Geranium mexicanum (aerial parts) and the aqueous extract of Bocconia frutescens showed the highest activity with inhibition values of 93.4 and 86.0%, respectively. The results obtained in this study give some scientific support to the use of the Mexican medicinal plants employed for the treatment of gastrointestinal disorders such as diarrhea.

  5. Screening of ectomycorrhizal fungi for degradation of polycyclic aromatic hydrocarbons

    Energy Technology Data Exchange (ETDEWEB)

    Braun-Luellemann, A.; Huettermann, A.; Majcherczyk, A. [Goettingen Univ. (Germany). Inst. fuer Forstbotanik

    2000-07-01

    Ectomycorrhizal fungi belonging to 16 species (27 strains) were tested for their ability to degrade polycyclic aromatic hydrocarbons (PAHs): Phenanthrene, chrysene, pyrene and benzo[a]pyrene. Cultivated on a complex liquid medium, most of the fungi tested were able to metabolise these compounds. Approximately 50% of the benzo[a]pyrene was removed by strains of Amanita excelsa, Leccinum versipelle, Suillus grevillei, S. luteus, and S. variegatus during a 4-week incubation period. The same amount of phenanthrene was also metabolised by A. muscaria, Paxillus involutus, and S. grevillei. The degradation of the other two PAHs was, for the most part, less effective. Only S. grevillei was able to remove 50% of the pyrene, whereas Boletus edulis and A. muscaria removed 35% of the chrysene. (orig.)

  6. Floristic and phytosociology in dense “terra firme” rainforest in the Belo Monte Hydroelectric Plant influence area, Pará, Brazil

    Directory of Open Access Journals (Sweden)

    DAN. Lemos

    Full Text Available Abstract The objective of the present study was to characterise the floristic and phytosociological composition on a stretch of dense “Terra Firme” rainforest located in the Belo Monte hydroelectric plant area of influence, located in the state of Pará, Brazil. All trees with DAP >10 cm situated in 75 permanent plots of 1 ha were inventoried. 27,126 individuals trees (361 ind.ha-1, distributed in 59 botanical families, comprising 481 species were observed. The families with the largest number of species were Fabaceae (94, Araceae (65 and Arecaceae (43, comprising 43.7% of total species. The species Alexa grandiflora (4.41, Cenostigma tocantinum (2.50 and Bertholletia excelsa (2.28 showed the highest importance values (IV. The ten species with greater IV are concentrated (22%. The forest community has high species richness and can be classified as diverse age trees, heterogeneous and of medium conservation condition.

  7. Avaliação da suscetibilidade à Xylella fastidiosa em diferentes espécies de cafeeiro Susceptibity valuation to Xylella fastidiosa in different coffee species

    Directory of Open Access Journals (Sweden)

    Rachel Benetti Queiroz-Voltan

    2005-01-01

    Full Text Available A bactéria Xylella fastidiosa Wells et al. foi detectada pela primeira vez em cafeeiro no Brasil, em 1995, entretanto acredita-se que a cultura foi infectada por essa bactéria há muitos anos, embora os sintomas fossem atribuídos a um estresse nutricional. Até o momento têm sido realizados estudos principalmente com espécies de C. arabica e C. canephora, porém, em outras espécies do gênero, somente foi detectada sua presença. Neste trabalho, objetivou-se avaliar a proporção de elementos de vaso do xilema obstruídos, total e parcialmente, pela X. fastidiosa, naturalmente infectadas, em diferentes espécies de cafeeiro do Banco de Germoplasma do IAC, visando identificar material resistente a essa bactéria para ser utilizado no programa de melhoramento genético. Os acessos estudados foram: C. canephora (progenitora da 'Guarini', C. liberica var. liberica, os quatro acessos de C. liberica var. dewevrei (Ugandae, Dibowskii, Abeokutae, Excelsa e o híbrido interespecífico Piatã (C. arabica X C. liberica var. dewevrei. Todos eles mostraram-se menos suscetíveis à X. fastidiosa. A porcentagem de obstrução dos elementos de vasos na folha não foi maior que 0,6% na maioria dos acessos, com exceção de Excelsa e do híbrido Piatã com até 2% de obstrução, sendo bem menos suscetíveis a essa bactéria do que as cultivares de C. arabica. Trata-se, portanto, de materiais genéticos importantes para serem utilizados no programa de melhoramento do cafeeiro visando à resistência ao agente dessa doença.Xylella fastidiosa Wells et al. bacteria was firstly detected in coffee plants in Brazil in 1995. However it is believed to be attacking this crop this time. Disease symptoms have been attributed mostly to nutritional unbalances. Up to date studies have comprised only the species C. arabica and C. canephora. However X. fastidiosa was also detected in other Coffea species, but without disease symptoms. Aiming to identify in the IAC

  8. Resistência natural de seis espécies de madeiras da região amazônica a fungos apodrecedores, em ensaios de laboratório

    Directory of Open Access Journals (Sweden)

    Marcus Vinicius Da Silva Alves

    2006-01-01

    Full Text Available This study evaluated the natural resistance of six Amazonian wood species: Aspidosperma desmanthum (Araracanga, Parinari excelsa (Parinari, Mouriri callocarpa (Miraúba, Marmaroxylon racemosum (Angelim-rajado, Peltogyne paniculata (Roxinho e Astronium sp. (Muiracatiara against Pycnoporus sanguineous, a white rot fungus, and Gloeophyllum trabeum, a brown rot fungus. Testing was performed based on the American Society for Testing and Materials - Standard Method for Accelerated Laboratory Test of Natural Decay Resistance of Woods - ASTM D2017/81(86. Results showed that all tested wood species were classified as very resistant to both decay fungi, except the wood of Aspidosperma desmanthum, which demonstrated to be very resistant to Pycnoporus sanguineous and resistant to Gloeophyllum trabeum. The wood of Peltogyne paniculata showed the best performance against Pycnoporus sanguineous, whereas the wood of Astronium sp. presented the best results when submitted to Gloeophyllum trabeum attack.

  9. Behavior of the natural and cultivated flora in the presence of air pollutant report. Etude du comportement de la flore sauvage ou cultivee a l'egard des pollutants

    Energy Technology Data Exchange (ETDEWEB)

    Bossavy, J.

    1971-01-01

    A complex study of the effects of air pollution by fluorine compounds on the vegetation in the Vallee de l Arc, Vallee de la Romanche, and Vallee de la Durance regions in France is presented. Iris, Veratrum, album, Picea excelsa, Pinus sylvestris, Abies alba, Polygonatum vulgare, Silene inflata. Gentiana lutea, Hypericum species, and Vaccinium myrtillus were the most sensitive species. Coniferous needles which tend to accumulate fluorine were most susceptible to fluorine which caused necrosis, premature loss of needles, and reduced growth. Vast expanses of dead coniferous forests were detected. The fluorine concentrations in the air, determined by means of exposed, calcium hydroxide-impregnated filter paper, ranged from 2.77 to 2.82 g/sq cm/day.

  10. Effect of electron beam irradiation on mechanical properties of gelatin/Brazil nut shell fiber composites

    International Nuclear Information System (INIS)

    Inamura, Patricia Y.; Shimazaki, Kleber; Moura, Esperidiana Augusta Barretos de; Mastro, Nelida L. del; Colombo, Maria Aparecida; Rosa, Ricardo de

    2010-01-01

    The use of natural fiber as polymeric matrix reinforcement has attracted interest, as fibers are renewable, of low cost, biodegradable and possesses non-toxic properties. In the present paper, Brazil nuts (Bertholletia excelsa) shell fiber (10% w/w) were mixed with gelatin (25% w/w), glycerin as plasticizer and acrylamide as copolymer to investigate the resultant mechanical properties effects upon ionizing radiation. The samples were irradiated at 40 kGy using a Dynamitron electron beam accelerator, at room temperature in the presence of air. The results showed that samples of gelatin with 10% of Brazil nuts shell fiber and irradiated at 40 kGy presented promising results for mechanical performance. (author)

  11. Chemical Compositions and Cytotoxic Activities of Leaf Essential Oils of Four Lauraceae Tree Species from Monteverde, Costa Rica

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    Maria C. Palazzo

    2009-01-01

    Full Text Available The leaf essential oils of four members of the Lauraceae Licaria excelsa, Licaria triandra, Perseaschiedeana, and Rhodostemonodaphne kunthiana, from Monteverde, Costa Rica, were obtained byhydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS. The leaf oil of L. excelsawas dominated by the monoterpenes a-pinene (42.9%, b-pinene (22.0% and myrcene (17.2%, while L.triandra was also rich in pinenes (40.9% and 28.5%, respectively. Persea schiedeana had considerableamounts of the sesquiterpenes d-cadinene (18.5%, a-copaene (15.1%, and (E-caryophyllene (13.3%.Rhodostemonodaphne kunthiana leaf oil had germacrene D (64.4% and bicyclogermacrene (17.6% as themajor components. The leaf essential oils were screened for in-vitro cytotoxic activity against MDA-MB-231and Hs 578T human tumor cells. R. kunthiana leaf oil showed notable activity against MDA-MB-231.

  12. Antioxidant activity of wild edible plants in the Black Sea Region of Turkey

    Energy Technology Data Exchange (ETDEWEB)

    Ozen, T.

    2010-07-01

    The anti oxidative activity of the 80% ethanol extract obtained from eleven commonly consumed wild edible plants was determined according to the phospho molybdenum method, reducing power, metal chelating, superoxide anion and free radical scavenging activity and compared to standard compounds such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and trolox. Total phenolic compounds, flavonoids, and anthocyanins in the extracts were also measured. Trachystemon orientalis, Vaccinium mrytillus, Rumex acetosella Polygonum amphibium, Beta vulgaris, and Similax Excelsa had the highest antioxidant capacities. Overall results showed that these plants can serve as good sources of bioactive polyphenols in the human diet and can be regarded as good candidates for nutritional supplement formulations due to their high concentrations of total phenolic compounds, flavonoids and anthocyanins as well as their strong antioxidant activity. (Author) 42 refs.

  13. Germination des semences, développement et croissance de quelques essences locales en zone forestière

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    Mapongmetsem, RM.

    1999-01-01

    Full Text Available Seed Germination, Growth and Development of some Local tree Species of the Forest Zone. Studies were conducted on eight indigenous multipurpose trees species : Alstonia boonei. De Wild, Ceiba pentandra (L. Gearth., Cordia platythyrsa Bark., Milicia excelsa (Welw ce Berg., Pycnanthus angolensis (Welw Warb., Ricinodendron heudelotii (Baill. Pierre ex Pax., Terminalia superba. Engl. et Diels et Triplochiton scleroxylon K. Schum. The objective of the study was to evaluate germination capacity, development and growth rate of the seedlings of the above mentionned species. The results showed that manual of seeds was clearly superior to the use of the other methods. For the initial growth, Ceiba pentandra and Ricinodendron heudelotii had the fastest growth. AH the species developped tap root system.

  14. Evaluation of selected Indian traditional folk medicinal plants against Mycobacterium tuberculosis with antioxidant and cytotoxicity study.

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    Tawde K. V

    2012-10-01

    Full Text Available Objective: To evaluate different solvent extracts of selected Indian traditional medicinal plant against Mycobacterium tuberculosis, its antioxidant potential and cytotoxicity. Methods: Acacia catechu (L. Willd (Root extract and Ailanthus excelsa Roxb., leaf extracts of Aegle marmelos Corr., Andrographis paniculata Nees. and Datura metel L. were sequentially extracted in water, ethanol, chloroform and hexane and evaluated for their anti-tuberculosis (TB activity against Mycobacterium tuberculosis using agar diffusion assay. The zone of inhibition ( at 20 and 40 mg/ ml was measured and MIC were calculated. The results were compared with Rifampicin as a standard anti TB drug. The extracts were also evaluated for DPPH and OH radical scavenging activities to understand their antioxidant potential. MTT based cytotoxicity assay was used for evaluating cytotoxicity of the selected samples against Chang liver cells. Results: The selected botanicals were sequentially extracted in water, ethanol, chloroform and hexane and tested for growth inhibition of M. tuberculosi. The hexane extract of A. catechu root and ethanol extract of A. paniculata leaf showed promising activity against M. tuberculosis while remaining extracts showed moderate anti TB activity. The samples were found to possess considerable DPPH and OH radical scavenging activities with no demonstrable cytotoxicity against Chang liver cells. Conclusions: Five traditional medicinal plants were selected for the present study. The selection of medicinal plants was based on their traditional usage for the treatment of tuberculosis, asthma and chronic respiratory diseases. Herein we report for the first time, the anti TB activity of root extracts of Acacia catechu and Ailanthus excelsa while leaf extract of Andrographis paniculata, Aegle marmelos and Datura metel. The study holds importance in the midst of multi drug resistance (MDR crisis in the TB management, since it unravels the scientific basis

  15. Alguns aspectos da anatomía-ecológica de Persea americana Mili (abacateiro e interpretações fisio-ecológicas

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    Moacyr Eurípedes Medri

    1985-11-01

    Full Text Available In the present work are presented anatomical differences in leaves situaded in different strata on the same tree ofPersea Americana. Analysis of linear regression and tests of coeficience of correlation demonstrate that in respect tb the relationships of the anatomical parameters studied, there is much interdependence. The histological point of view compares such parameters as number and size of stomata, volume of mesophyll, area of functional xylem, phloem, supportive tissue and epidermic tissue, and on the other han different microclimatic positions, domonstrates that Persea Americana is an extremely plastic species with much adaptability; as plastic as Hevea brasiliensis Muelli. Arg and much more plastic than Bertholletia excelsa Humb. & Benpl.No presente trabalho são apresentadas as diferenças anatômicas em folhas situadas em diferentes estratos de uma mesma árvore de Persea americana MUI, mostrando através de análise de regressão linear, coeficiente de correlação conclusivos a, respeito das relações diretas e significativas no tocante aos parâmetros anatômicos estudados, evidenciando interdependências dos mesmos. Do ponto de vista histológico, onde se tomou como parâmetro o número e tamanho de estômatos e volume do mesófilo, área xilemática útil, floema, tecido mecânico e epidérmico, e de outro lado, condições microclimáticas diferentes, P. americana evidencia ser uma espécie bastante plástica, havendo uma relação direta se correlacionada ao seu amplo comportamento adaptativo, sendo tão plástica quanto Hevea brasiliensis Muell. Arg. (Medri, 1977 e muito mais que Bertholletia excelsa Humb. & Benpl. (Medri, 1978.

  16. Contribuição ao conhecimento morfológico das espécies de leguminosae comercializadas no estado do Pará, como "angelim" Contribution to the morphologic knowledge of the species of leguminosae in the state of Pará, traded as "angelim"

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    Gracialda Costa Ferreira

    2004-01-01

    Full Text Available Sete espécies de Leguminosae comercializadas como "angelim", no estado do Pará, foram analisadas quanto aos aspectos morfológicos dos órgãos vegetativos e reprodutivos. Este estudo visou determinar diferenças básicas entre as espécies comercializadas com esta denominação vernacular, a fim de auxiliar no processo de identificação taxonômica das mesmas. Foi elaborada uma chave dicotômica para separar as espécies estudadas (Andira surinamensis, Dinizia excelsa, Hymenolobium excelsum, H. modestum, H. pulcherrimum, H. petraeum e Vatairea paraensis. As principais características utilizadas no campo, para separação das espécies estudadas, foram folha, folíolo, casca e tronco, porém, espécies de Andira, Hymenolobium e Vatairea, por apresentarem-se desprovidas de folhas no período fértil, necessitam de dados dos órgãos reprodutivos.The morphology of vegetative and reproductive structures of seven species of Leguminosae traded as "angelim" in the State of Pará were analyzed. This study sought to determine the basic differences between the species traded under the same vernacular name, in order to facilitate their taxonomic identification. A dichotomous key was developed to distinguish the species studied (Andira surinamensis, Dinizia excelsa, Hymenolobium excelsum, H. modestum, H. pulcherrimum, H. petraeum and Vatairea paraensis. The main characteristics used for separation in the field were of leaf, leaflet, bark and trunk. However, flower or fruit characters are needed to identify those species of Andira, Hymenolobium and Vatairea that lose their leaves while flowering.

  17. Antioxidant activity of wild edible plants in the Black Sea Region of Turkey

    Directory of Open Access Journals (Sweden)

    Özen, Tevfik

    2010-03-01

    Full Text Available The antioxidative activity of the 80% ethanol extract obtained from eleven commonly consumed wild edible plants was determined according to the phosphomolybdenum method, reducing power, metal chelating, superoxide anion and free radical scavenging activity and compared to standard compounds such as butylated hydroxyanisole (BHA, butylated hydroxytoluene (BHT and trolox. Total phenolic compounds, flavonoids, and anthocyanins in the extracts were also measured. Trachystemon orientalis, Vaccinium mrytillus, Rumex acetosella Polygonum amphibium, Beta vulgaris, and Similax Excelsa had the highest antioxidant capacities. Overall results showed that these plants can serve as good sources of bioactive polyphenols in the human diet and can be regarded as good candidates for nutritional supplement formulations due to their high concentrations of total phenolic compounds, flavonoids and anthocyanins as well as their strong antioxidant activity.La actividad antioxidante de extractos etanólicos al 80%, obtenidos de once plantas salvajes comúnmente consumidas, fue determinada por el método del fosfomolibdeno, poder reductor, actividad quelatante de metales, actividad captadora de aniones superóxidos y actividad captadora de radicales libres, y comparada con compuestos patrones tales como el butil hidroxianisol (BHA, butil hidroxitolueno (BHT y Trolox. El contenido de fenoles totales, flavonoides y antocianinas en el extracto fue también determinado. Trachystemon orientalis, Vaccinium mrytillus, Rumex acetosella Polygonum amphibium, Beta vulgaris, y Similax Excelsa tienen las capacidades antioxidantes más altas. En general, los resultados muestran que estas plantas pueden servir como una buena fuente de polifenoles bioactivos en la dieta humana, y pueden ser considerados como buenos candidatos para su uso como suplemento nutricional en formulaciones debido a su alta concentración en fenoles, flavonoides y antocinainas y a su fuerte actividad

  18. Morfologia da copa para avaliar o espaço vital de quatro espécies nativas da Amazônia Crown morphology to evaluate the growing space of four Amazon native species

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    Helio Tonini

    2005-07-01

    Full Text Available O objetivo deste trabalho foi o de definir diretrizes para o desbaste de quatro espécies nativas: a castanha-do-pará ou castanheira-do-brasil (Bertholletia excelsa, a andiroba (Carapa guianensis, o ipê-roxo (Tabebuia avellanedae e o jatobá (Hymenaea courbaril, plantadas em plantios homogêneos no Estado de Roraima. Foi feita a análise das relações entre os principais índices morfométricos da copa e o crescimento em diâmetro e altura; para isso foram medidos o diâmetro à altura do peito, altura total, altura de inserção e diâmetro da copa de 87 árvores. Na análise dos parâmetros de copa, a castanheira-do-brasil demonstrou superioridade em diâmetro e área, o que indica que essa espécie necessita de um maior espaço vital e maiores espaçamentos iniciais; no entanto, sua copa foi menos eficiente em manter um mesmo incremento médio anual em diâmetro.This work aimed at defining guidelines for thinning of four native species in pure stands, in Roraima State: Brazil nut tree (Bertholletia excelsa, Andiroba (Carapa guianensis, Ipê-roxo (Tabebuia avellanedae, and Jatobá (Hymenea courbaril. Analyses were made on the relations between main crown morphometric index, and diameter and height growth of these species. Eighty-seven trees were measured, and data taken are: diameter at breast height, total height, crown insertion height and crown diameter. Crown parameters analysis showed diameter and crown area superiority of Brazil nut, which indicates its need of a proper initial spacing and bigger growing space. However, Brazil nut crown was less efficient to maintain the same mean annual diameter increment.

  19. Screening of traditional antidiabetic medicinal plants of Mauritius for possible alpha-amylase inhibitory effects in vitro.

    Science.gov (United States)

    Kotowaroo, M I; Mahomoodally, M F; Gurib-Fakim, A; Subratty, A H

    2006-03-01

    In this study, seven exotic/indigenous medicinal plants of Mauritius, namely Coix lacryma-jobi (Poaceae), Aegle marmelos (Rutaceae), Artocarpus heterophyllus (Moraceae), Vangueria madagascariensis (Rubiaceae), Azadirachta indica (Meliaceae), Eriobotrya japonica (Rosaceae) and Syzigium cumini (Myrtaceae) were studied for possible effects on starch breakdown by alpha-amylase in vitro. The results showed that only Artocarpus heterophyllus significantly (p Artocarpus heterophyllus on alpha-amylase activity using rat plasma in vitro. It was found that the aqueous leaf extract significantly (p Artocarpus heterophyllus behaved as a competitive inhibitor. Results from the present study tend to indicate that Artocarpus heterophyllus could act as a 'starch blocker' thereby reducing post-prandial glucose peaks. Copyright 2006 John Wiley & Sons, Ltd.

  20. Pharmacognostical evaluation of Citrus jambhiri Lush. fruit

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    Swapnil Y Chaudhari

    2014-01-01

    Full Text Available Background: Citrus jambhiri Lush., commonly known as Jambīra Nimbū in Sanskrit is medium to large indigenous tree with spreading habit, less spiny than lemon and belonging to the family Rutaceae. In Ayurveda, it is used in many pharmaceutical procedures of purification (Śodhana, calcination (Māraṇa etc., Though it is an important plant, till date, no pharmacognostical reports have been available on its fruit. Materials and Methods: Study of fruit and its powder, histochemical tests and preliminary physicochemical investigations were done. Results and Conclusion: Results showed prismatic crystals of calcium oxalate, aerenchyma cells, oil globules, pitted vessels, scalariform vessels, juicy sac, etc., Preliminary physicochemical analysis revealed loss on drying (1.1%, ash value (1.4%, alcohol soluble extract (28.6%, and water soluble extract (53.3%. These observations can be of use in future studies.

  1. Antigenotoxic effects of Citrus aurentium L. fruit peel oil on mutagenicity of two alkylating agents and two metals in the Drosophila wing spot test.

    Science.gov (United States)

    Demir, Eşref; Kocaoğlu, Serap; Cetin, Huseyin; Kaya, Bülent

    2009-07-01

    Antigenotoxic effects of Citrus aurentium L. (Rutaceae) fruit peel oil (CPO) in combination with mutagenic metals and alkylating agents were studied using the wing spot test of D. melanogaster. The four reference mutagens, potassium dichromate (K2Cr2O7), cobalt chloride (CoCl2), ethylmethanesulfonate (EMS), and N-ethyl-N-nitrosourea (ENU) were clearly genotoxic. CPO alone at doses from 0.1 to 0.5% in Tween 80 was not mutagenic and did not enhance the mutagenic effect of the reference mutagens. However, antigenotoxic effects of CPO were clearly demonstrated in chronic cotreatments with mutagens and oil, by a significant decrease in wing spots induced by all four mutagens. The D. melanogaster wing spot test was found to be a suitable assay for detecting antigenotoxic effects in vivo. Copyright 2009 Wiley-Liss, Inc.

  2. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae in Brazil

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    A. L. Marsaro Júnior

    Full Text Available Abstract Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam. de Wit (Fabaceae, Morus nigra L. (Moraceae, Citrus reticulata Blanco (Rutaceae, Tectona grandis L. f. (Verbenaceae, Anacardium occidentale (Anacardiaceae, Annona squamosa L. and Xylopia aromatica (Lam. Mart. (Annonaceae, in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  3. Autecology of the common mormon butterfly, Papilio polytes (Lepidoptera : Rhopalocera : Papilionidae).

    Science.gov (United States)

    Atluri, J B; Ramana, S P Venkata; Reddi, C Subba

    2002-04-01

    The adults of the common mormon butterfly Papilio polytes Linn. feed on a variety of floral species. The larval food plants in the study area included Citrus limon and Murraya koenigii both of the family Rutaceae. The eggs are laid singly, and the hatching time is three days. The larvae pass through five instars. The larval growth is directly correlated with the quantity of food consumed. The AD (approximate digestibility) values decreased from first instar to the last, whereas the ECD (efficiency of conversion of digested food) and ECI (efficiency of conversion of ingested food) values increased, thus bearing an inverse relationship with AD. The development time from egg to adult is 28-30, giving 11-12 generations in a year, but with better breeding during August-February. Thus P. polytes is multivoltine.

  4. Pharmacognostical evaluation of Citrus jambhiri Lush. fruit.

    Science.gov (United States)

    Chaudhari, Swapnil Y; Harisha, C R; Galib, Ruknuddin; Prajapati, P K

    2014-01-01

    Citrus jambhiri Lush., commonly known as Jambīra Nimbū in Sanskrit is medium to large indigenous tree with spreading habit, less spiny than lemon and belonging to the family Rutaceae. In Ayurveda, it is used in many pharmaceutical procedures of purification (Śodhana), calcination (Māraṇa) etc., Though it is an important plant, till date, no pharmacognostical reports have been available on its fruit. Study of fruit and its powder, histochemical tests and preliminary physicochemical investigations were done. Results showed prismatic crystals of calcium oxalate, aerenchyma cells, oil globules, pitted vessels, scalariform vessels, juicy sac, etc., Preliminary physicochemical analysis revealed loss on drying (1.1%), ash value (1.4%), alcohol soluble extract (28.6%), and water soluble extract (53.3%). These observations can be of use in future studies.

  5. Evaluation of Antidepressant-like Effect of Citrus Maxima Leaves in Animal Models of Depression.

    Science.gov (United States)

    Potdar, Vikram H; Kibile, Swati J

    2011-09-01

    This study planned to assess antidepressant like activity of aqueous extract from leaves of Citrus maxima Merr. (Rutaceae). Boiling was used for aqueous extraction. Acute toxicity study was performed in mice. Antidepressant activity was studied using locomotor activity test, modified forced swimming test (FST) and tail suspension test (TST). Three doses 100, 200 and 300 mg/kg of aqueous extract of leaves were selected for testing. Fluoxetine (20 mg/kg, i.p.) and imipramine (30 mg/kg, i.p.) were used as the standard drugs. Aqueous extract of Citrus maxima leaves significantly reduced immobility time in both TST and FST. In locomotor activity testing it showed psychostimulant effect. Extract increased the climbing behavior in FST, which is similar to effect observed with imipramine. The results of this study suggest that antidepressant like effect of Citrus maxima seems to be mediated by an increase in norepinephrine level in synapses.

  6. Effect of light on contents of coumarin compounds in shoots of Ruta graveolens L. cultivated in vitro

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    Halina Ekiert

    2014-01-01

    Full Text Available Shoots of Ruta graveolens L. (Rutaceae were cultivated in stationary liquid culture under different light conditions: constant artificial light (900 lx, darkness, constant artificial light (900 ix following irradiation with UV-C light. The contents of five furanocoumarins: psoralen, bergapten, xanthotoxin, isopimpinellin and imperatorin, as well as biogenetic precursor of these metabolites, umbelliferone, were determined by HPLC method in shoots cultivated in vitro and in overground parts of plants growing in open air. It was shown that light conditions, tested in these experiments, significantly influenced contents of the metabolites in shoots cultivated in in vitro culture. Total content of the coumarin compounds in shoots cultivated under constant artificial light (900 lx was equal or higher than in plants growing under natural conditions. Therefore, it is suggested that stationary liquid shoot culture of R. graveolens. can be an alternative source for obtaining biologically active furanocoumarins.

  7. In vitro pollen germination of five citrus species

    International Nuclear Information System (INIS)

    Khan, S.A.; Perveen, A.

    2014-01-01

    The aim of present study is In vitro germination of the pollen grains of five Citrus species belonging to the family Rutaceae viz., Citrus aurantium L. var., aurantium Hook.f., C. limon (L.) Brum. f., C. paradisii Macfad, C. reticulata Blanco and C. sinensis (L.) Osbeck. using hanging drop technique. The germination was checked up to 48 weeks, for the pollen stored at different temperatures like 4 degree C, -20 degree C, -30 degree C and -60 degree C. The study indicates that low temperature and low relative humidity is better than high temperature and humidity with respect to pollen germination capacity and viability. Freeze dryer (-60 degree C) seems to be the best method to maintain pollen viability of stored pollen grains for a long period of time. Among five species Citrus aurantium, C. limon and C. sinensis showed high percentage of germination as compared to C. reticulata and C. paradisii. (author)

  8. An Overview on Citrus aurantium L.: Its Functions as Food Ingredient and Therapeutic Agent

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    Ipek Suntar

    2018-01-01

    Full Text Available Citrus aurantium L. (Rutaceae, commonly known as bitter orange, possesses multiple therapeutic potentials. These biological credentials include anticancer, antianxiety, antiobesity, antibacterial, antioxidant, pesticidal, and antidiabetic activities. The essential oil of C. aurantium was reported to display marked pharmacological effects and great variation in chemical composition depending on growing locations but mostly contained limonene, linalool, and β-myrcene. Phytochemically, C. aurantium is rich in p-synephrine, an alkaloid, and many health-giving secondary metabolites such as flavonoids. Animal studies have demonstrated a low affinity of p-synephrine for adrenergic receptors and an even lower affinity in human models. The present review focuses on the different biological activities of the C. aurantium in animal and human models in the form of extract and its pure secondary metabolites. Finally, it is concluded that both the extract and isolated compounds have no unwanted effects in human at therapeutic doses and, therefore, can confidently be used in various dietary formulations.

  9. Citrus bergamia essential oil: from basic research to clinical application

    Directory of Open Access Journals (Sweden)

    Michele eNavarra

    2015-03-01

    Full Text Available Citrus bergamia Risso et Poiteau, also known as Bergamot, is a plant belonging to the Rutaceae family, defined as a hybrid of bitter orange and lemon. It is an endemic plant of the Calabria region (Italy. Bergamot fruit is primarily used for the extraction of its essential oil (bergamot essential oil: BEO, employed in perfume, cosmetics, food and confections.The aim of this review was to collect recent data from the literature on Citrus bergamia essential oil and, through a critical analysis, focus on safety and the beneficial effects on human health. Clinical studies on the therapeutic applications of BEO exclusively focus on the field of aromatherapy, suggesting that its use can be useful for reducing anxiety and stress.

  10. First record of Crypticerya zeteki (Cockerell, 1914 (Monophlebidae in Brazil and Maconellicoccus hirsutus (Green, 1908 (Pseudococcidae in the state of Maranhão

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    A. S. J. C. Ramos

    2017-06-01

    Full Text Available Abstract Crypticerya zeteki (Cockerell, 1914 (Hemiptera: Coccomorpha: Monophlebidae is recorded for the first time from Brazil and Maconellicoccus hirsutus (Green, 1908 (Hemiptera: Coccomorpha: Pseudococcidae is recorded for the first time from the state of Maranhão, Brazil. Both species were collected from branches, leaves and fruits of various fruit trees in the municipalities of São José de Ribamar, São Luís and Paço do Lumiar, Maranhão, Brazil. Crypticerya zeteki was collected on Citrus spp. (Rutaceae, Cocos nucifera (L. (Arecaceae, Cycas revoluta L. (Cycadaceae, Malpighia punicifolia L. (Malpighiaceae, Mangifera indica L. (Anacardiaceae, Musa paradisiaca L. (Musaceae and Theobroma grandiflorum Schum (Malvaceae, all first records for this species. Maconellicoccus hirsutus was collected on Spondias tuberosa Arruda (Anacardiaceae and M. punicifolia L. (Malpighiaceae, both new records for this species.

  11. Larvicidal activity and structure activity relationship of cinnamoyl amides from Zanthoxylum armatum and their synthetic analogues against diamondback moth, Plutella xylostella.

    Science.gov (United States)

    Kumar, Vishal; Reddy, S G Eswara; Bhardwaj, Anuja; Dolma, Shudh Kirti; Kumar, Neeraj

    2016-01-01

    Cinnamoyl amides isolated from Zanthoxylum armatum (Rutaceae) and their synthetic analogues were tested for their insecticidal activity against the second instar larvae of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) to determine the promising structures with insecticidal activity. Most of the test compounds showed promising activity against larvae of P. xylostella. However, the activities of different compounds varied depending on the presence of different substituents at various positions of both the aromatic rings A and B. Among the tested compounds, 8, N-(3-bromo-4-methoxyphenethyl)cinnamamide showed best larvicidal activity with an LC50 = 62.13 mg/L followed by 6, N-(3׳-bromophenethyl)cinnamamide (LC50=128.49 mg/L) and 2 N-(4׳-methoxyphenylethyl)cinnamamide (LC50 = 225.65 mg/L).

  12. Uso tradicional de plantas medicinales en la vereda San Isidro, municipio de San José de Pare-Boyacá, Colombia: un estudio preliminar usando técnicas cuantitativas

    Directory of Open Access Journals (Sweden)

    Jarvis Yamith Toscano González

    2005-07-01

    se deben tener al ser administradas. Los datos fueron analizados mediante el Índice de Valor de Uso (IVUs y el Nivel de Uso Significativo de TRAMIL. Se registraron 35 especies de plantas de uso medicinal, distribuidas en 20 familias, representadas en su mayoría por Asteraceae, Lamiaceae, Solanaceae y Rutaceae. Se reportaron 11 especies con un mayor valor de importancia en medicina tradicional. La documentación de los usos de las plantas medicinales en el área de influencia, revela que el conocimiento tradicional continúa profundamente arraigado entre la comunidad y se mantiene el saber popular a manos de curanderos y madres cabeza de familia.

  13. Antimicrobial activity of grapefruit seed and pulp ethanolic extract.

    Science.gov (United States)

    Cvetnić, Zdenka; Vladimir-Knezević, Sanda

    2004-09-01

    Antibacterial and antifungal activity of ethanolic extract of grapefruit (Citrus paradisi Macf., Rutaceae) seed and pulp was examined against 20 bacterial and 10 yeast strains. The level of antimicrobial effects was established using an in vitro agar assay and standard broth dilution susceptibility test. The contents of 3.92% of total polyphenols and 0.11% of flavonoids were determined spectrometrically in crude ethanolic extract. The presence of flavanones naringin and hesperidin in the extract was confirmed by TLC analysis. Ethanolic extract exibited the strongest antimicrobial effect against Salmonella enteritidis (MIC 2.06%, m/V). Other tested bacteria and yeasts were sensitive to extract concentrations ranging from 4.13% to 16.50% (m/V).

  14. Molecular docking for thrombolytic activity of some isolated compounds from Clausena lansium.

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    Arkajyoti Paul

    2017-03-01

    Full Text Available Clausena lansium (Family- Rutaceae is commonly known as wampee, is found in fallow lands throughout Bangladesh. Our aim of the study to performed molecular docking studies to identify potential binding affinities of the phytocompounds from Clausena lansium, namely Clausemarin B, Clausenaline C, Clausenaline E, Murrayanine, vanillic acid and Xanthotoxol for searching of lead molecule for thrombolytic activity. A wide range of docking score found during molecular docking by Schrodinger. Clausemarin B , Clausenaline C , Clausenaline E, Murrayanine , vanillic acid and Xanthotoxol showed the docking score -6.926, -4.041, -4.889 , -4.356, -3.007 and -5.816 respectively. Among all the compounds Clausemarin B showed the best docking score. So, Clausemarin B is the best compounds for thrombolytic activity, as it possessed the best value in Molecular docking. Further in vivo investigation need to identify the thrombolytic activity of isolated compounds from Clausena lansium.

  15. Combinatorial synthesis by nature: volatile organic sulfur-containing constituents of Ruta chalepensis L.

    Science.gov (United States)

    Escher, Sina; Niclass, Yvan; van de Waal, Matthijs; Starkenmann, Christian

    2006-09-01

    Ongoing interest in discovering new natural fragrance and flavor ingredients prompted us to examine a solvent extract of sulfurous-sweaty smelling Ruta chalepensis L. (Rutaceae) plant material more closely. Twenty-one sulfur-containing constituents of similar structures were identified by GC/MS techniques. Amongst them, 14 have never been described to occur in nature. The compounds 1-18 belong to a family of natural flavor and fragrance molecules having a 1,3-positioned O,S moiety in common. The identities of the natural constituents were confirmed by comparison with synthetic reference samples, and the organoleptic properties of the latter were studied. The relative and absolute configurations of the four stereoisomers of 4-methyl-3-sulfanylhexan-1-ol (5) were established by stereoselective synthesis. The natural isomers consisted of a 65 : 35 mixture of (3R,4S)-5 and (3S,4S)-5.

  16. Umbelliferone: Sources, chemistry and bioactivities review

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    Ofentse Mazimba

    2017-12-01

    Full Text Available Umbelliferone is a 7-hydroxycoumarin that is a pharmacologically active agent. It is widely distributed within the Rutaceae and Apiaceae (Umbelliferae families and is efficiently extracted using methanol. Umbelliferone is a fluorescing compound used as a sunscreen agent. It is synthesized using the Pechmann condensation reaction of resorcinol and formyl acetic acid. Biosynthetically it is synthesized using the phenylpropanoid pathway. Umbelliferone is a synthon for other coumarins and heterocycles with improved biological activities. In the Literature modest antibacterial and antifungal activities are reported with MIC values of 500–1000 μg/mL, but exhibited good E. coli anti-biofilm formation. Umbelliferone shows good inhibitions of DPPH, hydroxyl, superoxide anion and ABTS radicals. Other reported activities are anti-inflammatory, anti-hyperglycaemic, molluscicidal and anti-tumor activities. Keywords: Umbelliferone, Anti-bacterial, Anti-tumor, Cytotoxicity, Synthesis, Biosynthesis

  17. ALCALOIDES ACRIDÔNICOS INIBEM CATEPSINA L E V

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    Emerson F. Marques

    2016-01-01

    Full Text Available Cathepsins represent a class of enzymes that has the primary function of randomly degrading proteins in the lysosomes, although are also involved in different pathologies. The aim of this paper was to evaluate the capacity of acridone alkaloids isolated from Swinglea glutinosa (Rutaceae to inhibit cathepsin L in vitro . The IC50 values found were in the 0.8-57 µM range and the most promising compounds were alkaloids 1 and 2, with IC50 of 0.9 and 0.8 µM, respectively. Enzyme kinetics revealed that they are reversible competitive inhibitors with respect to the substrate Z-FR-MCA. This small series of acridone alkaloids showed low selectivity for both cathepsins, but represent promising lead candidates for the further development of competitive cathepsin L and V inhibitors.

  18. Notes on the genus Paramignya: Phytochemistry and biological activity

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    Ninh The Son

    2018-06-01

    Full Text Available Genus Paramignya belongs to Rutaceae family, with interesting secondary metabolites, comprising main classes of compounds coumarin and coumarin glycosides, acridone alkaloids, tirucallane and tirucallane glycosides, phenols, and flavonoids, as well as several compounds limonoid, lignin glycoside and sterol. Paramignya species has been employing as folk medicines against hepatitis, diabetes, cancer, nose infections. Many bioactive reported such as cytotoxic assay, antioxidant, antiinflammatory, antiumor cancer, α-glucosidase inhibitory activities indicated either Paramignya extracts, fractions, or isolated compounds to become valuable resources for natural new drug developments. However, no evidences are reported for general view about this genus. In current paper, we exhibit overview almost of isolated components and biological evaluations from this genus. These findings are important to improve the values of these medicinal plants for the health benefit, drug discovery and guideline for future researches.

  19. Inhibitory effect of rhetsinine isolated from Evodia rutaecarpa on aldose reductase activity.

    Science.gov (United States)

    Kato, A; Yasuko, H; Goto, H; Hollinshead, J; Nash, R J; Adachi, I

    2009-03-01

    Aldose reductase inhibitors have considerable potential for the treatment of diabetic complications, without increased risk of hypoglycemia. Search for components inhibiting aldose reductase led to the discovery of active compounds contained in Evodia rutaecarpa Bentham (Rutaceae), which is the one of the component of Kampo-herbal medicine. The hot water extract from the E. rutaecarpa was subjected to distribution or gel filtration chromatography to give an active compound, N2-(2-methylaminobenzoyl)tetrahydro-1H-pyrido[3,4-b]indol-1-one (rhetsinine). It inhibited aldose reductase with IC(50) values of 24.1 microM. Furthermore, rhetsinine inhibited sorbitol accumulation by 79.3% at 100 microM. These results suggested that the E. rutaecarpa derived component, rhetsinine, would be potentially useful in the treatment of diabetic complications.

  20. First report of Maconellicoccus hirsutus (Green, 1908) (Hemiptera: Coccoidea: Pseudococcidae) and the associated parasitoid Anagyrus kamali Moursi, 1948 (Hymenoptera: Encyrtidae), in Brazil.

    Science.gov (United States)

    Marsaro Júnior, A L; Peronti, A L B G; Penteado-Dias, A M; Morais, E G F; Pereira, P R V S

    2013-05-01

    The pink hibiscus mealybug (PHM), Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) and the associated hymenopterous parasitoid, Anagyrus kamali Moursi, 1948 (Hymenoptera: Encyrtidae), are reported for the first time in Brazil. Specimens of the PHM were collected on nine hosts plants, Annona muricata L. (Anonnaceae), Glycine max (L.) Merr. (Fabaceae), Centrolobium paraensis Tul. (Fabaceae), Inga edulis Mart. (Fabaceae), Hibiscus rosa-sinensis L. (Malvaceae), Psidium guajava L. (Myrtaceae), Averrhoa carambola L. (Oxalidaceae), Citrus sinensis (L.) Osbeck (Rutaceae) and Solanum lycopersicum L. (Solanaceae), in four municipalities in the north-northeast of the state of Roraima. The plants C. paraensis, I. edulis and C. sinensis are recorded for the first time as a hosts for PHM. Characteristic injuries observed on the host plants infested by PHM and suggestions for its management are presented.

  1. New Umami Amides: Structure-Taste Relationship Studies of Cinnamic Acid Derived Amides and the Natural Occurrence of an Intense Umami Amide in Zanthoxylum piperitum.

    Science.gov (United States)

    Frerot, Eric; Neirynck, Nathalie; Cayeux, Isabelle; Yuan, Yoyo Hui-Juan; Yuan, Yong-Ming

    2015-08-19

    A series of aromatic amides were synthesized from various acids and amines selected from naturally occurring structural frameworks. These synthetic amides were evaluated for umami taste in comparison with monosodium glutamate. The effect of the substitution pattern of both the acid and the amine parts on umami taste was investigated. The only intensely umami-tasting amides were those made from 3,4-dimethoxycinnamic acid. The amine part was more tolerant to structural changes. Amides bearing an alkyl- or alkoxy-substituted phenylethylamine residue displayed a clean umami taste as 20 ppm solutions in water. Ultraperformance liquid chromatography coupled with a high quadrupole-Orbitrap mass spectrometer (UPLC/MS) was subsequently used to show the natural occurrence of these amides. (E)-3-(3,4-Dimethoxyphenyl)-N-(4-methoxyphenethyl)acrylamide was shown to occur in the roots and stems of Zanthoxylum piperitum, a plant of the family Rutaceae growing in Korea, Japan, and China.

  2. Efeitos da temperatura e do alimento no desenvolvimento de Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae Effects of temperature and food on the development of Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae

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    Fábio Souto Almeida

    2007-12-01

    Full Text Available Dysdercus maurus Distant, 1901 (Hemiptera, Pyrrhocoridae é uma importante praga de Gossypium spp. (algodoeiro, Citrus Sinensis Osbeck (Rutaceae (laranjeira e Citrus reticulata (Rutaceae (tangerineira, além de sementes de Chorisia speciosa St. Hil. (paineira. Este trabalho objetivou avaliar os efeitos da temperatura e do alimento no desenvolvimento de D. maurus. Foram realizados oito tratamentos, seis em que os percevejos foram alimentados com sementes de paineira e mantidos a 15, 18, 20, 25 e 30 ± 1ºC, UR 80 ± 3% e fotofase de 12 h ou em condições ambientais de laboratório (23,5 ± 2,6ºC, UR 73,3 ± 9,9 %, e dois em que foram alimentados com sementes de algodão variedade IAC-22 e mantidos a 25 e 30ºC. Em todos os tratamentos foram observados cinco estágios imaturos. O aumento da temperatura proporcionou diminuição do tempo de desenvolvimento. A temperatura de 15ºC foi letal para ovos e ninfas de D. maurus. A menor mortalidade de ninfas ocorreu quando os percevejos foram alimentados com sementes de algodão a 25ºC (24,07%. A menor temperatura base (Tb foi obtida para o 1º ínstar (11,54ºC e a maior para o 2º ínstar (15,33ºC. As fêmeas de D. maurus necessitam de maior quantidade de graus-dias (329,93 graus-dias que os machos (300,49 graus-dias para atingir o estádio adulto.Dysdercus maurus Distant, 1901 (Hemiptera, Pyrrhocoridae is an important pest on Gossypium spp. (cotton tree, Citrus sinensis Osbeck (Rutaceae and Citrus reticulata Blanco (Rutaceae crops. This insect also feeds on seeds of Chorisia speciosa St. Hil. (Bombacaceae. This work aimed to evaluate the effects of temperature and food on the development of D. maurus. Eight treatments were carried out, in six of them bugs were fed with seeds of C. speciosa and kept at 15, 18, 20, 25 and 30 ± 1ºC, 80 ± 3% RH and 12h photophase or in laboratory conditions (23.5 ± 2.6ºC, 73.3 ± 9.9 % RH, and in the other two treatments bugs were fed with seeds of cotton variety

  3. Ethnobotanical study of some Ghanaian anti-malarial plants.

    Science.gov (United States)

    Asase, Alex; Oteng-Yeboah, Alfred A; Odamtten, George T; Simmonds, Monique S J

    2005-06-03

    An ethnobotanical study was conducted in the Wechiau Community Hippopotamus Sanctuary area in Ghana, through interviews and quadrate studies, to investigate the range and abundance of species used in the treatment of malaria. Forty-one species belonging to 17 families were encountered during the study. Of the 17 families studied Leguminosae and Anacardiaceae predominated in terms of number of species used to treat malaria. Eight plant species namely, Afraegle paniculata (Rutaceae), Haematostaphis barteri (Anacardiaceae), Indigo era pulchra (Leguminosae), Monanthotaxis sp. (Annonaceae), Ozoroa insignis (Anacardiaceae), Strychnos innocua (Loganiaceae), Strychnos spinosa (Loganiaceae) and Xeroderris stuhlmannii (Leguminosae) have not previously been documented for the treatment of malaria in Ghana. The results are discussed and recommendations made for future research to support the conservation and sustainable harvesting of the species reported to have medicinal properties.

  4. Management of Fruit Species in Urban Home Gardens of Argentina Atlantic Forest as an Influence for Landscape Domestication.

    Science.gov (United States)

    Furlan, Violeta; Pochettino, María L; Hilgert, Norma I

    2017-01-01

    Home gardens are considered germplasm repositories and places for experimentation, thus they are key sites for the domestication of plants. Domestication is considered a constant process that occurs along a continuum from wild to managed to domesticated populations. Management may lead to the modification of populations and in other cases to their distribution, changing population structure in a landscape. Our objective is focused on the management received in home gardens by perennial species of fruits. For this, the management practices applied to native and exotic perennial fruits species by a group of 20 women in the periurban zone of Iguazú, Argentina, were analyzed. In-depth interviews were conducted, as well as guided tours for the recognition and collection of specimens of species and ethnovarieties. Sixty-six fruit species managed in the home gardens were recorded. The predominant families are Rutaceae, Myrtaceae, and Rosaceae. The fruit species with the highest number of associated management practices are pitanga ( Eugenia uniflora ) and pindó ( Syagrus rommanzoffiana ). The 10 species with the highest management intensity are (in decreasing order of intensity) banana ( Musa x paradisiaca ), palta ( Persea americana ), pitanga ( E. uniflora ), mango ( Mangifera indica ), cocú ( Allophylus edulis ), mamón ( Carica papaya ), guayaba ( Psidium guajava ), limón mandarina ( Citrus x taitensis ), güembé ( Philodendron bipinnatifidum ), and mandarina ( Citrus reticulata ). Among the families with the greatest modifications in their distribution, abundance and presence of ethnovarieties in domestic gardens, are the native Myrtaceae and the exotic Rutaceae. The main management practices involved are cultivation, tolerance, transplant and enhancement in decreasing order. It can be concluded that in Iguazú, fruit species management shows both in plant germplasm as in environment a continuum that through tolerance, transplant and cultivation latu sensu has

  5. Management of Fruit Species in Urban Home Gardens of Argentina Atlantic Forest as an Influence for Landscape Domestication

    Directory of Open Access Journals (Sweden)

    Violeta Furlan

    2017-09-01

    Full Text Available Home gardens are considered germplasm repositories and places for experimentation, thus they are key sites for the domestication of plants. Domestication is considered a constant process that occurs along a continuum from wild to managed to domesticated populations. Management may lead to the modification of populations and in other cases to their distribution, changing population structure in a landscape. Our objective is focused on the management received in home gardens by perennial species of fruits. For this, the management practices applied to native and exotic perennial fruits species by a group of 20 women in the periurban zone of Iguazú, Argentina, were analyzed. In-depth interviews were conducted, as well as guided tours for the recognition and collection of specimens of species and ethnovarieties. Sixty-six fruit species managed in the home gardens were recorded. The predominant families are Rutaceae, Myrtaceae, and Rosaceae. The fruit species with the highest number of associated management practices are pitanga (Eugenia uniflora and pindó (Syagrus rommanzoffiana. The 10 species with the highest management intensity are (in decreasing order of intensity banana (Musa x paradisiaca, palta (Persea americana, pitanga (E. uniflora, mango (Mangifera indica, cocú (Allophylus edulis, mamón (Carica papaya, guayaba (Psidium guajava, limón mandarina (Citrus x taitensis, güembé (Philodendron bipinnatifidum, and mandarina (Citrus reticulata. Among the families with the greatest modifications in their distribution, abundance and presence of ethnovarieties in domestic gardens, are the native Myrtaceae and the exotic Rutaceae. The main management practices involved are cultivation, tolerance, transplant and enhancement in decreasing order. It can be concluded that in Iguazú, fruit species management shows both in plant germplasm as in environment a continuum that through tolerance, transplant and cultivation latu sensu has derived in a

  6. Native and introduced host plants of Anastrepha fraterculus and Ceratitis capitata (Diptera: Tephritidae) in northwestern Argentina.

    Science.gov (United States)

    Ovruski, Sergio; Schliserman, Pablo; Aluja, Martín

    2003-08-01

    Wild or commercially grown, native and exotic fruit were collected in 30 localities in the Tucumán province (NW Argentina) from January 1990 to December 1995 to determine their status as hosts of Anastrepha fraterculus (Wiedemann) and/or Ceratitis capitata (Wiedemann), the only two fruit fly species of economic and quarantine importance in Argentina. A total of 84,094 fruit (3,466.1 kg) representing 33 species (7 native and 26 exotic) in 15 plant families were sampled. We determined the following 17 host plant associations: Annona cherimola Miller (Annonaceae), Citrus paradisi Macfadyn (Rutaceae), Diospyros kaki L. (Ebenaceae), Eugenia uniflora L., Psidium guajava L., Myrcianthes pungens (Berg) Legrand (Myrtaceae), Ficus carica L. (Moraceae), Juglans australis Grisebach (Juglandaceae), Mangifera indica L. (Anacardiaceae), Eriobotrya japonica (Thunb.) Lindl., Prunus armeniaca L., P. domestica L., and P. persica (L.) Batsch (Rosaceae) were infested by both A. fraterculus and C. capitata. Citrus aurantium L., Citrus reticulata Blanco, Citrus sinensis (L.) Osbeck (Rutaceae), and Passiflora caerulea L. (Passifloraceae) were only infested by Ceratitis capitata. Out of a total of 99,627 adults that emerged from pupae, 69,180 (approximately 69.5%) were Anastrepha fraterculus, 30,138 (approximately 30.2%) were C. capitata, and 309 (approximately 0.3%) were an unidentified Anastrepha species. Anastrepha fraterculus predominated in native plant species while C. capitata did so in introduced species. Infestation rates (number of larvae/kg of fruit) varied sharply from year to year and between host plant species (overall there was a significant negative correlation between fruit size and infestation level). We provide information on fruiting phenology of all the reported hosts and discuss our findings in light of their practical (e.g., management of A. fraterculus and C. capitata in citrus groves) implications.

  7. Furoquinoline Alkaloids and Methoxyflavones from the Stem Bark of Melicope madagascariensis (Baker T.G. Hartley

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    Vincent E. Rasamison

    2016-09-01

    Full Text Available Abstract Melicope madagascariensis (Rutaceae is an endemic plant species of Madagascar that was first classified as a member of the genus Euodia J. R. & G. Forst (Rutaceae under the scientific name Euodia madagascariensis Baker. Based on morphological characteristics, Thomas Gordon Hartley taxonomically revised E. madagascariensis Baker to be M. madagascariensis (Baker T.G. Hartley. Chemotaxonomical studies have long been used to help the identification and confirmation of taxonomical classification of plant species and botanicals. Aiming to find more evidences to support the taxonomical revision performed on E. madagascariensis, we carried out phytochemical investigation of two samples of the plant. Fractionation of the ethanol extracts prepared from two stem bark samples of M. madagascariensis (Baker T.G. Hartley led to the isolation of seven known furoquinoline alkaloids 1–7 and two known methoxyflavones 8 and 9. The presence of furoquinoline alkaloids and methoxyflavones in the title species is in agreement with its taxonomic transfer from Euodia to Melicope. Antiprotozoal evaluation of the isolated compounds showed that 6-methoxy-7-hydroxydictamnine (heliparvifoline, 3 showed weak antimalarial activity (IC50 = 35 µM against the chloroquine-resistant strain Dd2 of Plasmodium falciparum. Skimmianine (4 displayed moderate cytotoxicity with IC50 value of 1.5 µM against HT-29 colon cancer cell line whereas 3,5-dihydroxy-3′,4′,7-trimethoxyflavone (9 was weakly active in the same assay (IC50 = 13.9 µM. Graphical Abstract

  8. Grapefruit as a host for the West Indian fruit fly (Diptera: Tephritidae).

    Science.gov (United States)

    Mangan, Robert L; Thomas, Donald B; Moreno, Aleena Tarshis; Robacker, David

    2011-02-01

    The most common hosts for the West Indian fruit fly, Anastrepha obliqua (Macquart) (Diptera: Tephritidae) are fruit in the family Anacardiaceae (mango [Mangifera L.] and mombin [Spondias L.] species). However, similar to many of the tropical fruit flies of major economic importance, this species attacks several other families of crop fruit, including Annonaceae (cherimoya, Annona cherimola Mill.), Myrtaceae (guava, Psidium L.), Oxalidaceae (carambola, Averrhoa carambola L.), Passifloraceae (granadilla, Passiflora quadrangularis Mill.), and Sapotaceae [mamey sapote, Pouteria sapota (Jacq.) H. E. Moore & Steam]. In the family Rutaceae the economically important genus Citrus has been reported and until recently considered a host for this fruit fly. In this study, we reviewed the taxonomy of A. obliqua, tested specific chemicals that may inhibit oviposition, compared egg-to-adult survival of A. obliqua on preferred hosts and on grapefruit (Citrus X paradisi Macfad.), and measured fruit tissue-specific developmental rates of A. obliqua and the known citrus breeding Mexican fruit fly, Anastrepha ludens (Loew) (Diptera: Tephritidae), from egg to pupae. Our literature review shows much confusion concerning the taxonomy of this and related Anastrepha species, including synonymies and confusion with other species. The deterrent effect of the highest concentration of flavonoids for oviposition, although significant, was not absolute. Experiments carried out under laboratory conditions showed 15-40 times greater survival of A. ludens (whose preferred hosts include Rutaceae) on grapefruit compared with A. obliqua for both tree attached and harvested fruit. Experiments of survival of developing stages over time showed that the two species oviposit into different tissues in the fruit, and mortality is much higher for the West Indian fruit fly in the flavedo and albedo of the fruit compared with the Mexican fruit fly.

  9. Selection of Reference Genes for Expression Studies in Diaphorina citri (Hemiptera: Liviidae).

    Science.gov (United States)

    Bassan, Meire Menezes; Angelotti-Mendonc A, Je Ssika; Alves, Gustavo Rodrigues; Yamamoto, Pedro Takao; Moura O Filho, Francisco de Assis Alves

    2017-12-05

    The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is considered the main vector of the bacteria associated with huanglongbing, a very serious disease that has threatened the world citrus industry. The absence of efficient control management protocols, including a lack of resistant cultivars, has led to the development of different approaches to study this pathosystem. The production of resistant genotypes relies on D. citri gene expression analyses by RT-qPCR to assess control of the vector population. High-quality, reliable RT-qPCR analyses depend upon proper reference gene selection and validation. However, adequate D. citri reference genes have not yet been identified. In the present study, we evaluated the genes EF 1-α, ACT, GAPDH, RPL7, RPL17, and TUB as candidate reference genes for this insect. Gene expression stability was evaluated using the mathematical algorithms deltaCt, NormFinder, BestKeeper, and geNorm, at five insect developmental stages, grown on two different plant hosts [Citrus sinensis (L.) Osbeck (Sapindales: Rutaceae) and Murraya paniculata (L.) Jack (Sapindales: Rutaceae)]. The final gene ranking was calculated using RefFinder software, and the V-ATPase-A gene was selected for validation. According to our results, two reference genes are recommended when different plant hosts and developmental stages are considered. Considering gene expression studies in D. citri grown on M. paniculata, regardless of the insect developmental stage, GAPDH and RPL7 have the best fit as reference genes in RT-qPCR analyses, whereas GAPDH and EF 1-α are recommended as reference genes in insect studies using C. sinensis. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  10. Efectos inducidos por Ruta graveolens L., Cnidoscolus chayamansa McVaugh y Citrus aurantium L. sobre los niveles de glucosa, colesterol y triacilglicéridos en un modelo de rata diabética

    Directory of Open Access Journals (Sweden)

    Lauro Figueroa-Valverde

    Full Text Available Varias plantas con propiedades hipoglucemicas se han utilizado en medicina popular y sistemas curativos tradicionales en todo el mundo. El propósito de este trabajo fue evaluar los efectos inducidos por Ruta graveolens L., Rutaceae, Cnidoscolus chayamansa McVaugh, Euphorbiaceae, y Citrus aurantium L., Rutaceae, en un modelo de rata diabética, a la que se le cuantificaron los niveles de glucosa cada 24 horas por un mes después de la administración gástrica del extracto de las plantas. Además, el colesterol y los triglicéridos fueron evaluados usando técnicas enzimáticas. Los resultados mostraron que la administración de Cnidoscolus chayamansa a dosis de 0.5 a 1.5 g/kg induce un aumento hipoglucemico ( 200 mg/dL. Otros resultados, mostraron que Citrus aurantium ejerce cambios en la concentración de triacilglicéridos (158-172 mg/dL y colesterol (120-128 mg/dL. Finalmente, la administración de Ruta graveolens a dosis de 0.5 g/kg induce un efecto hipoglucemico (< 200 mg/dL. Además, Ruta graveolens a dosis de 0.5 a 1.5 g/kg induce variaciones en los niveles de triacilglicéridos (110-120 mg/dL y colesterol (116-124 mg/dL. En conclusión la administración de Cnidoscolus chayamansa ejerce efectos hipoglucemicos en una manera dosis dependiente en comparación con Ruta graveolens y Citrus aurantium. Además, las plantas evaluadas inducen cambios en los niveles de lípidos dependiente de la dosis.

  11. Radioecological studies of tritium movement in a tropical rain forest

    Energy Technology Data Exchange (ETDEWEB)

    Martin, J R; Jordan, C F; Koranda, J J; Kline, J R [Bio-Medical Division, Lawrence Radiation Laboratory, University of California, Livermore, CA (United States)

    1970-05-01

    Several experiments on the movement of tritium in a tropical ecosystem have been conducted in the montane rainforest of Eastern Puerto Rico by the Bio-Medical Division of the Lawrence Radiation Laboratory, Livermore, in cooperation with the Puerto Rico Nuclear Center. Tritiated whaler was used as a tracer for water movement in: a) mature evergreen trees of the climax rainforest; b) soil and substory vegetation and c) rapidly growling successional species. A feasibility study on the Atlantic Pacific Interoceanic Canal is currently being conducted. If thermonuclear explosives were used in constructing the canal, tritium would be deposited as tritiated water and distributed among the several biological compartments of the tropical ecosystem in that area. The main hydrogen compartments are water in the soil and in leaves, limbs and wood of forest trees. Organic tissue hydrogen comprises another compartment. In the tree experiment, tritiated water was injected directly into several species of mature, broad leaved evergreen tropical trees. Transpiration and residence time for tritium was determined from analyses of leaves sampled during a several month period. Transpiration ranged from 4 ml/day/gm dry leaf for an understory Dacryodes excelsa to 10.0 and 13.8 ml/day/gm dry leaf for a mature Sloanea berteriana and D. excelsa, respectively. Mean residence time for the S. berteriana was 3.9 {+-} 0.2 days and the understory and mature D. excelsa values were 9.5 {+-} 0.4 and 11.0 {+-} 0. 6 days, respectively. In another experiment, tritiated water was sprinkled over a 3.68 m{sup 2} plot and its movement down into the soil and up into the vegetation growing on the plot was traced. The pattern of water movement in the soil was clearly demonstrated. The mean residence time for tritium in the soil and in trees was found to be 42 {+-} 2 days and 67 {+-} 9 days, respectively. The residence time for tritium in the trees in this experiment was considerably longer than for the single

  12. Radioecological studies of tritium movement in a tropical rain forest

    International Nuclear Information System (INIS)

    Martin, J.R.; Jordan, C.F.; Koranda, J.J.; Kline, J.R.

    1970-01-01

    Several experiments on the movement of tritium in a tropical ecosystem have been conducted in the montane rainforest of Eastern Puerto Rico by the Bio-Medical Division of the Lawrence Radiation Laboratory, Livermore, in cooperation with the Puerto Rico Nuclear Center. Tritiated whaler was used as a tracer for water movement in: a) mature evergreen trees of the climax rainforest; b) soil and substory vegetation and c) rapidly growling successional species. A feasibility study on the Atlantic Pacific Interoceanic Canal is currently being conducted. If thermonuclear explosives were used in constructing the canal, tritium would be deposited as tritiated water and distributed among the several biological compartments of the tropical ecosystem in that area. The main hydrogen compartments are water in the soil and in leaves, limbs and wood of forest trees. Organic tissue hydrogen comprises another compartment. In the tree experiment, tritiated water was injected directly into several species of mature, broad leaved evergreen tropical trees. Transpiration and residence time for tritium was determined from analyses of leaves sampled during a several month period. Transpiration ranged from 4 ml/day/gm dry leaf for an understory Dacryodes excelsa to 10.0 and 13.8 ml/day/gm dry leaf for a mature Sloanea berteriana and D. excelsa, respectively. Mean residence time for the S. berteriana was 3.9 ± 0.2 days and the understory and mature D. excelsa values were 9.5 ± 0.4 and 11.0 ± 0. 6 days, respectively. In another experiment, tritiated water was sprinkled over a 3.68 m 2 plot and its movement down into the soil and up into the vegetation growing on the plot was traced. The pattern of water movement in the soil was clearly demonstrated. The mean residence time for tritium in the soil and in trees was found to be 42 ± 2 days and 67 ± 9 days, respectively. The residence time for tritium in the trees in this experiment was considerably longer than for the single injected input

  13. Studies in American orchids VII Studies in American orchids VII

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    Garay Leslie A.

    1968-09-01

    Full Text Available Vanilla rubra (Lam. Urb. in Fedde Rep. Beih, 5: 157, 1920 / Elleanthus Kalbreyeri Garay, nom. nov. / Elleanthus lancifolius Presl, Rel. Haenk. 97, 1827 / Pleurothallis cardiothallis Rchb. f. in Bot. Zeit. 15: 158, 1857 / Pleurothallis excelsa Garay, sp. nov. / Platystele Johnstonii (Ames Garay, comb. nov. / Platystele propinqua (Ames, Garay, comb. nov. / Platystele xiphochila (Rchb. f. Garay, comb. nov. / Lepanthopsis serrulata (Cogn. Hespenheide & Garay, comb. nov. / Masdevallia bulbophyllopsis Krzl. in Fedde Rep. 17: 412, 1921. / Masdevallia heteroptera Rchb. f. in Gard. Chron. n. s. 3: 590, 1875. / Masdevallia velutina  Rchb. f. in Gard. Chron. n. s. 4: 420, 1875 / Epidendrum bracteolatum Presl, ReI. Haenk. 100, 1827 / Epidendrum infaustum Rchb. f. in Hamb. Gartenz. 19: 13, 1863 / Epidendrum jejunum Rchb. f. in Otia Bot. Hamb, 12, 1878 / Epidendrum vernixium Rchb. f. & Warsc. in Bonpl. 2: 110, 1854 / Maxillaria ochracea (Rchb. f. Garay, comb. nov / Odontoglossum narthecioides (Krzl. Garay, comb. nov. / Sigmatostalix auriculata, Garay, sp. nov. / Sigmatostalix  Brownii Garay,· sp. nov / Rusbyella pusilla (C. Schweinf. Garay, comb. nov / Sphyrastylis ecuadorensis Garay, sp. nov / Pterostemma  calceolaris Garay, sp. nov.Vanilla rubra (Lam. Urb. in Fedde Rep. Beih, 5: 157, 1920 / Elleanthus Kalbreyeri Garay, nom. nov. / Elleanthus lancifolius Presl, Rel. Haenk. 97, 1827 / Pleurothallis cardiothallis Rchb. f. in Bot. Zeit. 15: 158, 1857 / Pleurothallis excelsa Garay, sp. nov. / Platystele Johnstonii (Ames Garay, comb. nov. / Platystele propinqua (Ames, Garay, comb. nov. / Platystele xiphochila (Rchb. f. Garay, comb. nov. / Lepanthopsis serrulata (Cogn. Hespenheide & Garay, comb. nov. / Masdevallia bulbophyllopsis Krzl. in Fedde Rep. 17: 412, 1921. / Masdevallia heteroptera Rchb. f. in Gard. Chron. n. s. 3: 590, 1875. / Masdevallia velutina  Rchb. f. in Gard. Chron. n. s. 4: 420, 1875 / Epidendrum bracteolatum Presl, ReI. Haenk. 100, 1827

  14. [Contribution of tropical upland forests to carbon storage in Colombia].

    Science.gov (United States)

    Yepes, Adriana; Herrera, Johana; Phillips, Juan; Galindo, Gustavo; Granados, Edwin; Duque, Alvaro; Barbosa, Adriana; Olarte, Claudia; Cardona, María

    2015-03-01

    The tropical montane forests in the Colombian Andean region are located above 1500 m, and have been heavily deforested. Despite the general presumption that productivity and hence carbon stocks in these ecosystems are low, studies in this regard are scarce. This study aimed to (i) to estimate Above Ground Biomass (AGB) in forests located in the South of the Colombian Andean region, (ii) to identify the carbon storage potential of tropical montane forests dominated by the black oak Colombobalanus excelsa and to identify the relationship between AGB and altitude, and (iii) to analyze the role of tropical mountain forests in conservation mechanisms such as Payment for Environmental Services (PES) and Reducing Emissions from Deforestation and Degradation (REDD+). Twenty six 0.25 ha plots were randomly distributed in the forests and all trees with D > or =10 cm were measured. The results provided important elements for understanding the role of tropical montane forests as carbon sinks. The information produced can be used in subnational initiatives, which seek to mitigate or reduce the effects of deforestation through management or conservation of these ecosystems, like REDD+ or PES. The AGB and carbon stocks results obtained were similar to those reported for lowland tropical forests. These could be explained by the dominance and abundance of C. excelsa, which accounted for over 81% of AGB/carbon. The error associated with the estimates of AGB/carbon was 10.58%. We found a negative and significant relationship between AGB and altitude, but the higher AGB values were in middle altitudes (approximatly = 700-1800 m), where the environmental conditions could be favorable to their growth. The carbon storage potential of these forests was higher. However, if the historical rate of the deforestation in the study area continues, the gross emissions of CO2e to the atmosphere could turn these forests in to an important emissions source. Nowadays, it is clear that tropical

  15. Umidade relativa de equilibrio e oxidação de lipídeos em farinhas de castanha do Pará, de macadâmia e de soja Equilibrium relative humidity and lipid oxidation in Brazil-nut, macadamia nut and soybean seed flours

    Directory of Open Access Journals (Sweden)

    L.G. do Prado-Filho

    1994-08-01

    Full Text Available Foi estudada a oxidação de lipídeos adicionados a farinhas de castanha do Pará (Bertholleta excelsa, de macadâmia (Macadâmia integrifolia e de soja (Glycine max equilibradas nas atividades de água (Aa 0,51; 0,57; 0,67; 0,75; 0,79 e 0,81, a 35°C. O substrato utilizado para quantificar tanto a oxidação autocatalítica como a oxidação enzímica foi o óleo de soja na proporção de 20% (p:p. Dois mecanismos de oxidação de lipídeos concorrem pelo substrato nas condições estudadas. A baixos valores de Aa - de 0,51 a 0,75 - o mecanismo mais eficiente é a autoxidação devida à maior exposição do substrato ao oxigênio e à menor mobilidade dos reagentes - enzima e lipídeo - nas reações de natureza enzímica. Em valores de Aa maiores - 0,79 e 0,81 - predomina a oxidação enzímica, e atua a proteção do substrato pela água, frente à ação do oxigênio. O índice de peróxido medido no transcurso de 6 dias apresenta máximos e mínimos devidos a reações secundárias atuando sobre produtos das reações primárias.Lipid oxidation was studied on the flour of Brazil-nut (Bertolleta excelsa, macadâmia nut (Macadamia integrifoliá and soybean seed (Glycine max, in enviroments with controlled water activity (Aw values of 0.51; 0.57; 0.67; 0.75; 0.79 and 0.81 at 35°C. Every 24 hours during 6 days the peroxide value was determined for each Aw in flour with enzyme inactivation (110°C, 2 hours as well as in flour without inactivation. At low Aw values (up to 0.75 the oxidation by oxigen is the most effective mechanism of deterioration of the lipids. At higher water activity values (0.79 and 0.81 the protective effect of the humidity upon the lipids and the greater mobility of the reagents make the activity of the lipoxigenase the most important mechanism of lipid deterioration.

  16. Estudo anatômico das espécies de Leguminosae comercializadas no estado do Pará como "angelim" An anatomic study of Leguminosae species in the State of Pará commercialized as "angelim"

    Directory of Open Access Journals (Sweden)

    Gracialda Costa Ferreira

    2004-09-01

    Full Text Available A estrutura da madeira de sete espécies de Leguminosae comercializadas como "angelim", no estado do Pará, foi analisada quanto aos aspectos anatômicos através de cortes histológicos da madeira, nos sentidos transversal, tangencial e radial. Através deste estudo procurou-se determinar diferenças básicas entre as espécies comercializadas com a mesma denominação vernacular, a fim de auxiliar no processo de identificação anatômica das mesmas. Uma chave dicotômica foi elaborada para separar as espécies estudadas (Andira surinamensis, Dinizia excelsa, Hymenolobium excelsum, H. modestum, H. pulcherrimum, H. petraeum e Vatairea paraensis. Características de parênquima, raios e poros são muito úteis na separação das espécies estudadas em nível de gênero, porém em nível de espécie a separação é mais difícil. Para o agrupamento de espécies, é necessário que as características anatômicas das espécies sejam conhecidas para associadas a características morfológicas.The wood structure of seven commercialized species of Leguminosae as angelim, in the state of Pará, was analyzed by their anatomical aspects and through histological sections of the wood, in transversal, tangential and radial planes. This study was undertaken to determine basic differences among various species commercialized with the same vernacular denomination, in order to facilitate the process of their anatomical identification. A dichotomous key was elaborated to separate the species studied (Andira surinamensis, Dinizia excelsa, Hymenolobium excelsum, H. modestum, H. pulcherrimum, H. petraeum and Vatairea paraensis. Characteristics of parenchyma, rays and vessels are very useful in the separation of the species studied in the genus level, however in the species level the separation is more difficult. For the species grouping, it is necessary that the anatomical characteristics of the species are known for associates to the morphologic characteristics.

  17. Vegetative rhythm of some woody species; Rythmes de vegetation de quelques especes ligneuses

    Energy Technology Data Exchange (ETDEWEB)

    Gagnaire, J [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

    1965-07-01

    In laboratory conditions from march 1963 to april 1964, variations of calcium absorption by roots and translocation speed towards aerial parts of young norway spruce (Picea excelsa) in hydroponic growing, present fixed stages in a vegetative cycle: 1. from early april until end may a rapid rising of minerals from roots to young parts of the tree, associated with a redistribution of calcium formerly fixed in tissues; 2. in June-July, an equilibrium in mineral exchanges between the different parts of the tree; 3. from early august to end of October, a pre-dormancy phase marked by a slowing of translocation; 4. in november-december, an absolute dormancy period; 5. from January to march, a post-dormancy phase in which root absorption and translocation to aerial parts recover. In outdoors conditions and for a determined climate these stages start and end on dates linked to species and variety: - in Grenoble, absolute dormancy starts in September for thuyas, early October for maple-trees, middle October for norway spruce and novembers for poplars. (authors) [French] De mars 1963 a avril 1964, les variations de l'absorption radiculaire des sels de calcium et de leur vitesse de transport vers les parties aeriennes de jeunes epiceas (Picea excelsa) en culture hydroponique, au laboratoire, montrent cinq phases distinctes dans un cycle vegetatif: 1. du debut avril a la fin mai, une phase de montee rapide des sels mineraux depuis les racines jusqu'aux parties jeunes de l'arbre, associee a une redistribution des sels de calcium deja fixes dans les tissus; 2. en juin, juillet une phase d'equilibre dans les echanges mineraux entre les differentes parties de l'arbre; 3. du debut aout a la fin octobre une phase de pre-dormance caracterisee par un ralentissement progressif de la vitesse de transport; 4. en novembre et decembre, une phase de dormance absolue; 5. de janvier a mars une phase de post-dormance au cours de laquelle l'absorption radiculaire et le transport vers les parties

  18. Sobrevivência de espécies arbóreas plantadas em clareiras causadas pela colheita de madeira em uma floresta de terra firme no município de Paragominas na Amazônia brasileira Survival of seedlings planted in gaps after harvesting in a terra firme rain forest in Paragominas region in the Brazilian Amazonia

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    Jaqueline Macêdo Gomes

    2010-03-01

    Full Text Available Analisou-se a sobrevivência de mudas plantadas em 400 clareiras causadas por exploração florestal de impacto reduzido, em floresta de terra firme na Amazônia Oriental. Foram plantadas 3.818 mudas de 17 espécies, das quais apenas Schizolobium amazonicum não ocorre na área de estudo. A distância entre as mudas plantadas foi de aproximadamente 5m. As avaliações ocorreram em 2005 e 2006. Com base na sobrevivência das mudas aos 11 meses após o plantio, as espécies indicadas para o enriquecimento de clareiras são: Schizolobium amazonicum, Cedrela odorata, Jacaranda copaia, Manilkara huberi, Astronium gracile, Pouteria bilocularis, Tabebuia impetiginosa,Pseudopiptadenia suaveolens, Cordia goeldiana, Parkia gigantocarpa, Simarouba amara, Sterculia pilosa, Laetia procera, Dinizia excelsa e Schefflera morototoni. Estudos sobre a taxa de crescimento, em períodos mais longos, são necessários para confirmar a utilização dessas espécies em plantios de enriquecimento de clareiras oriundas de exploração florestal, como alternativa para aumentar a produtividade e o valor econômico das florestas naturais manejadas na Amazônia brasileira.Survival of seedlings planted in 400 gaps created by reduced impact logging in a terra firme forest in the Eastern Amazonia was evaluated. 3,818 seedlings from 17 species occurring in the study area, except for Schizolobium amazonicum (paricá, which is rare in natural forests of Paragominas region, were planted in the gaps. Spacing of planted seedlings was 5m. According to survival of seedlings during 11 months after planting, the species Schizolobium amazonicum, Cedrela odorata, Jacaranda copaia, Manilkara huberi, Astronium gracile, Pouteria bilocularis, Tabebuia impetiginosa,Pseudopiptadenia suaveolens, Cordia goeldiana, Parkia gigantocarpa, Simarouba amara, Sterculia pilosa, Laetia procera, Dinizia excelsa and Schefflera morototoni can be suggested for enriching in gaps created by reduced impact logging

  19. Contribución de los bosques tropicales de montaña en el almacenamiento de carbono en Colombia

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    Adriana Yepes

    2015-03-01

    Full Text Available Los bosques tropicales de montaña (BTM en la región Andina de Colombia se encuentran por encima de 1 500m, y han sido fuertemente deforestados. A pesar de la presunción general de que la productividad y las reservas de carbono en estos ecosistemas son bajas, los estudios al respecto son escasos. Este estudio tuvo por objetivo i estimar la biomasa aérea (BA de los bosques localizados en el sur de la región Andina colombiana, ii identificar el potencial de almacenamiento de carbono en bosques dominados por el roble negro Colombobalanus excelsa e identificar la relación entre la BA y la altitud, y iii analizar su papel en mecanismos de conservación, como el Pago por Servicios Ambientales (PSA y la Reducción de Emisiones por deforestación y Degradación (REDD+. Veintiséis parcelas de 0.25ha fueron distribuidas al azar, y se midieron todos los árboles con D≥10cm. Los resultados obtenidos de BA y carbono almacenado fueron similares a los reportados para bosques tropicales de tierras bajas. Esto se puede explicar por la abundancia y dominancia de C. excelsa, que contribuye con más del 81% de la BA/carbono; el error asociado a las estimaciones fue de 10.58%. Se encontró una relación negativa y significativa entre la BA y la altitud, pero los valores más altos de BA estuvieron en las altitudes medias (≈1 700-1 800m, donde las condiciones ambientales podrían ser favorables para su crecimiento. El potencial de almacenamiento de carbono de estos bosques fue alto. Los resultados permiten tener elementos importantes para entender el papel de los BTM como sumideros de carbono. Sin embargo, si la tasa histórica de deforestación en el área de estudio continúa, las emisiones brutas de CO2e a la atmósfera podrían convertirlos en una fuente de emisiones importante. Actualmente, los BTM son vulnerables a la deforestación, especialmente los bosques de roble negro debido a su valor comercial. Teniendo en cuenta el alto potencial de

  20. Fitoseídeos (Acari: Phytoseiidae associados a cafezais e fragmentos florestais vizinhos Phytoseiids (Acari: Phytoseiidae associated to coffee plantations and adjacent forest fragments

    Directory of Open Access Journals (Sweden)

    Ester Azevedo Silva

    2010-10-01

    Full Text Available Existem poucas informações sobre a fauna de ácaros predadores (Phytoseiidae em ambientes naturais brasileiros adjacentes a agroecossistemas cafeeiros (Coffea spp. ou sobre a influência que essa vegetação exerce como reservatório de ácaros predadores. Neste estudo, objetivou-se avaliar a diversidade destes organismos em cafeeiros e fragmentos florestais adjacentes. Coletaram-se amostras das espécies Calyptranthes clusiifolia (Miq. O. Berg (Myrtaceae, Esenbeckia febrifuga (A. St.-Hil. A. Juss. ex Mart. (Rutaceae, Metrodorea stipularis Mart. (Rutaceae e Allophylus semidentatus (Miq. Radlk. (Sapindaceae, em oito fragmentos florestais, de 5 a 51 ha, e cafezais adjacentes, nos meses de junho (final período chuvoso e outubro (final período seco nos anos 2004 e 2005, na região Sul do Estado de Minas Gerais. Ácaros foram extraídos das folhas, utilizando o método de lavagem e, em seguida, montados em lâminas de microscopia em meio de Hoyer, para identificação específica. No total foram identificados 2.348 fitoseídeos, sendo 2.090 nos fragmentos florestais e 258 espécimes nos cafezais adjacentes, pertencentes a 38 espécies. Servindo-se de análise faunística, a espécie Iphiseiodes zuluagai Denmark & Muma, 1972 apresentou os melhores índices no agroecossistema cafeeiro, sendo muito frequente e constante nas épocas estudadas. Nos fragmentos florestais Amblyseius herbicolus Chant, 1959, Iphiseiodes affs. neonobilis Denmark & Muma, 1978, Leonseius regularis DeLeon, 1965 e Euseius alatus DeLeon, 1966 foram dominantes, muito abundantes, muito frequentes e constantes nas épocas estudadas. Podemos concluir que a vegetação nativa abriga ácaros predadores, inimigos naturais de ácaros-praga, que ocorrem na cultura cafeeira, possibilitando o desenvolvimento de programas de manejo ecológico com áreas de vegetação natural e agroecossistemas cafeeiros adjacentes.There is little information about the fauna of predatory mites

  1. High resolution taxonomic study of the late Eocene (~34 Ma) Florissant palynoflora, Colorado, USA

    Science.gov (United States)

    Bouchal, J. M.

    2012-04-01

    .g. Fagaceae, Malvaceae, Onagraceae, Rosaceae, Rutaceae, Platanaceae), to group taxa into representative vegetation units, and to estimate the palaeoclimate. Preliminary results show that the Fagaceae are represented by at least 5 species (including two different Quercus types), the Malvaceae, Rosaceae and Rutaceae are represented by 3 species, and the Onagraceae and the Platanaceae by 2 species. The method used has also allowed the new discovery of the following: rarly occurring pollen grains of Asteraceae, and relatively small grains of Tetracentron (Trochodendraceae).

  2. Moscas frugívoras associadas a mirtáceas e laranjeira "Céu" na região do Vale do Rio Caí, Rio Grande do Sul, Brasil Frugivorous flies in myrtaceans and orange trees 'Céu' in the region of Vale do Rio Caí, Rio Grande do Sul State, Brazil

    Directory of Open Access Journals (Sweden)

    Tacimara Gattelli

    2008-02-01

    Full Text Available Este estudo teve o objetivo de reconhecer as espécies de moscas frugívoras em mirtáceas: Eugenia uniflora L., Campomanesia xanthocarpa Berg., Psidium cattleianum Sabine, Psidium guajava L. e Acca sellowiana (Berg. Burret., bem como em Citrus sinensis (L. Osbeck (Rutaceae, na região do Vale do Caí, RS. Os frutos foram coletados no período de maturação de cada espécie frutífera, entre outubro de 2004 e julho de 2005, levados ao laboratório e acondicionados em potes com areia mantidos a 25 ± 1°C; 80 ± 10% UR e fotofase de 12 horas. Os pupários obtidos foram individualizados e suas características foram registradas. De Tephritidae foi registrada apenas Anastrepha fraterculus (Wiedemann e de Lonchaeidae Neosilba n. sp. 3, Neosilba zadolicha McAlpine & Steyskal e Neosilba certa (Walker. As duas últimas são novos registros para o Rio Grande do Sul. Apenas em P. cattleianum foram registradas todas as espécies de moscas encontradas neste trabalho. Os resultados evidenciam que A. fraterculus é a espécie de mosca-das-frutas de maior ocorrência para a região do Vale do Caí, RS, nas frutíferas estudadas.This study aimed at recognizing frugivorous flies species in Myrtaceae: Eugenia uniflora L., Campomanesia xanthocarpa Berg. Psidium cattleianum Sabine, Psidium guajava L. and Acca sellowiana (Berg. Burret., as well as in Citrus sinensis (L. Osbeck (Rutaceae, at Vale do Cai region, Rio Grande do Sul. Fruits were collected at the maturation stage of each fruit species between October 2004 and July 2005, and at the laboratory they were placed in pots with sand and were kept at 12h photoperiod, 80 ± 10% RH and 25 ± 1°C. Pupae were individualized and their characteristics were recorded. In Tephritidae only Anastrepha fraterculus (Wied. was recorded and in Lonchaeidae, Neosilba n. sp. 3, Neosilba zadolicha McAlpine & Steyskal and Neosilba certa (Walker. These last two species were recorded for the first time in Rio Grande do Sul. Only on

  3. Insecticide effects of Ruta graveolens, Copaifera langsdorffii and Chenopodium ambrosioides against pests and natural enemies in commercial tomato plantation = Ação inseticida de Ruta graveolens, Copaifera langsdorffii e Chenopodium ambrosioides sobre pragas de tomate

    Directory of Open Access Journals (Sweden)

    Flávia Silva Barbosa

    2011-01-01

    Full Text Available The aim of this study was to evaluate the insecticide effect of watery leaf extracts of Ruta graveolens (Rutaceae, alcoholic leaf extracts of Copaifera langsdorffii (Caesalpinaceae and Chenopodium ambrosioides (Chenopodiaceae in the concentration of 5% under field conditions. The experiment design was randomized blocks with six replications. The parcels treated with plant extracts showed reduction in the population of pests when compared with the controlparcels. The extract elaborated with C. langsdorffii presented greater insecticidal effect under Bemisia tabaci (Hemiptera: Aleyrodidae and sum of pests. It was verified that after 24 hours ofspraying, the parcels treated with the extract of C. ambrosioides presented minor numbers of adults of Tuta absoluta (Lepidoptera: Gelechiidae, followed by the parcels treated with extract of R.graveolens. There were smaller numbers of parasitoid eggs of epidopterans Trichogramma sp. (Hymenoptera: Trichogrammatidae and sum of natural enemies (predators + parasitoids in the parcels that had received spraying with extracts from the plants of C. langsdorffii and C. ambrosioides, followed by R. graveolens, compared to the control. There were a smaller number of parasitoids from the family Eulophidae (Hymenoptera attacking caterpillars of T. absoluta in plants treated with R. graveolens, followed by C. langsdorffii and C. ambrosioides than in the control.O objetivo deste trabalho foi avaliar o efeito inseticida dos extratos aquosos de folhas de Ruta graveolens (Rutaceae e alcoólicos de folhas de Copaifera langsdorffii (Caesalpinaceae e de folhas de Chenopodium ambrosioides (Chenopodiaceae a 5%. O experimento foi em blocos casualizados com seis repetições. As parcelas tratadas tiveram redução na população de pragas, quando comparadas às parcelas sem nenhum tratamento. O extrato elaborado com C. langsdorffii apresentou maior efeito inseticida em Bemisia tabaci (Hemiptera: Aleyrodidae e soma das pragas

  4. Métodos de extração e concentrações no efeito inseticida de Ruta graveolens L., Artemisia verlotorum Lamotte e Petiveria alliacea L. a Diabrotica speciosa Germar Extraction methods and concentrations for the insecticidal effect of Ruta graveolens L., Artemisia verlotorum Lamotte, and Petiveria alliacea L. against Diabrotica speciosa Germar

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    F.S. Barbosa

    2009-01-01

    Full Text Available O objetivo deste trabalho foi determinar o melhor método de extração e concentração para Ruta graveolens L. (Rutaceae, Artemisia verlotorum Lamotte (Asteraceae e Petiveria alliacea L. (Phytolaccaceae quanto ao efeito inseticida a Diabrotica speciosa Germar (Coleoptera: Chrysomelidae. Os extratos que utilizaram óleo de soja comercial, pelo menos como um dos solventes, apresentaram maior mortalidade de D. speciosa em suas testemunhas (solvente = óleo, nas três plantas estudadas, em relação às suas concentrações. O extrato aquoso de R. graveolens, a 5% de concentração, apresentou maior mortalidade corrigida de D. speciosa (32,5% que os extratos de A. verlotorum em água (10% de concentração (20,3% e P. alliacea em álcool etílico (2% de concentração (12,5%. O método de extração com água é simples, sendo passível de utilização por pequenos agricultores. A R. graveolens é planta facilmente cultivada, sendo, portanto, boa alternativa de controle dessa praga.The aim of this work was to determine the best extraction method and concentration for Ruta graveolens L. (Rutaceae, Artemisia verlotorum Lamotte (Asteraceae and Petiveria alliacea L. (Phytolaccaceae concerning their insecticidal effect against Diabrotica speciosa Germar (Coleoptera: Chrysomelidae. Extracts using commercial soybean oil as at least one of the solvents resulted in higher D. speciosa mortality in controls (solvent = oil of the three studied plants, relative to their treatment concentrations. R. graveolens extract in water, at 5% concentration, showed higher D. speciosa corrected mortality (32.5% than A. verlotiorum extracts in water (10% concentration (20.3% and P. alliacea in ethanol (2% concentration (12.5%. The extraction method with water is simple and can be used by small farmers. R. graveolens is an easily cultivated plant and constitutes, therefore, a good alternative to control this plague.

  5. Perspectivas do ecoturismo: uma análise sobre características e percepções locais na Floresta Nacional Saracá-Taquera, Pará - Brasil

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    Fagno Tavares Oliveira

    2016-01-01

    Full Text Available Herein we present data on social profile, economic activities and sources of income of local communities in Saracá-Taquera National Forest, in Pará State, Brazil, analyzing perceptions and expectations related to ecotourism and nature conservation issues. We conduct social surveys, using forms to 134 households. Net income of the local population was estimated by the balance between revenue and expenditure. We used the residuals of the regression to estimate variation in income between families. The local population has a low educational level, most of the families live in the region for over 15 years, and the main economic activities are cassava flour production (Manihot esculenta Crantz and Brazilian nut extraction (Bertholletia excelsa H. & B. Lecythidaceae. The revenues generated from the monthly sale of Brazilian nuts (about US$ 115 and manioc flour (U$ 64 covered monthly expenses with basic needs and work equipment. However, the net monthly income is very low (US$ 6 over a period up to five months. The acquisition and maintenance of work equipment are the biggest expenses (U$ 36, influencing the decrease in total monthly income. The benefit transferred by Bolsa Familia, a social policy of the Brazilian Government, contributes significantly (R² = 0.63 to increase the income of local people. The inclusion of ecotourism in the locality can support the entry of revenue, complementing the local income, reducing the pressure on Brazilian nuts trees and promoting nature conservation.

  6. Occurrence of leguminous trees

    Energy Technology Data Exchange (ETDEWEB)

    Kirkbride, J.H.; Arkcoll, D.B.A.; Turnbull, J.W.; Magalhaes, L.M.S.; Fernandes, N.P.

    1984-01-01

    Five papers from the symposium are presented. Kirkbride, J.H. Jr.; Legumes of the cerrado. pp 23-46 (Refs. 55) A review is given. Some 548 legume species in 59 genera are listed that have been reported from cerrado vegetation. Felker, P.; Legume trees in semi-arid and arid areas. pp 47-59 (Refs. 41) A review is given of worldwide research activities. Arkcoll, D.B.; A comparison of some fast growing species suitable for woodlots in the wet tropics. pp 61-68 (Refs. 9) Studies are described near Manaus on intensive silviculture (for fuelwood production) of Eucalyptus deglupta, Cedrelinga catanaeformis (catenaeformis), Jacaranda copaia, and Inga edulis. Turnbull, J.W.; Six phyllodinous Acacia species for planting in the humid tropical lowlands. pp 69-73 (Refs. 14) Distribution, ecology, growth, and utilization are described for A. auriculiformis, A. mangium, A. aulacocarpa, A. crassicarpa, A. cincinnata, and A. polystachya. Magalhaes, L.M.S., Fernandes, N.P.; Experimental stands of leguminous trees in the Manaus region. pp 75-79 (Refs. 8) Performance up to age 20 yr of Cedrelinga catenaeformis, Dalbergia nigra, Dinizia excelsa, Dipteryx odorata, Dipteryx sp., Diplotropis sp., Eperua bijuga, Pithecellobium racemosum, Vouacapoua pallidior, and Hymenaea sp. is described.

  7. MIDDLE TRIASSIC AUTOCLASTIC DEPOSITS FROM SOUTHWESTERN BOSNIA AND HERZEGOVINA

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    Duje Smirčić

    2016-06-01

    Full Text Available Middle Triassic volcanic and volcaniclastic rocks have been recognized at several localities near Bosansko Grahovo, in southwestern part of Bosnia and Herzegovina in the External Dinarides. Peculiar types of autoclastic rocks were investigated. These are peperites and hyaloclastites. Regarding specific structures, mineral composition and micropetrographic characteristics it was possible to further differentiate hyaloclastites into in situ hyaloclastites and slightly resedimented hyaloclastites that represent genetic succession. All rock types occurred in a deep sea troughs that formed as a consequence of Middle Triassic extensional tectonic and rift related wrench faulting. In situ hyaloclastites and slightly resedimented hyaloclastites were formed due to quenching at the contact of lava effusions with sea water. Genesis of peperites is related to lava emplacement in unconsolidated water saturated lime mudstones that were deposited in deep sea basin. All investigated rock types represent first findings of autoclastic deposits in the External Dinarides. Biostratigraphic constraints achieved by means of conodont species Neogondolella excentrica, Paragondolella excelsa, Paragondolella trammeri and Gladigondolella tethydis indicate Late Anisian to Early Ladinian interval of the autoclastic deposits from Bosansko Grahovo.

  8. An apple plus a Brazil nut a day keeps the doctors away: antioxidant capacity of foods and their health benefits.

    Science.gov (United States)

    Ferrari, Carlos Kusano Bucalen; Percário, Sandro; Silva, José Carlos Costa Baptista; da Silva Torres, Elizabeth Aparecida Ferraz

    2016-01-01

    Antioxidant-rich foods scavenge free radicals and other reactive species, decreasing the risk of different non-communicable chronic diseases. The objective of this study was to review the content of total antioxidant capacity of commonly foods comparing with experimental data and to explore the health benefits due to foods with moderate to high TAC. The TAC was analytically measured using the "Total Antioxidant Capacity" (NX2332) test from Randox® (UK) by spectrometry at 600 nm. Brazil nut (Bertholletia excelsa), "guaraná" (Paullinia cupana Kunth) powder, ready to drink boiled coffee (Coffea arabica L.), and milk chocolate (made from seeds of Theobroma cacao) had the highest TAC values, followed by collard greens (Brassica oleracea L.), beets (Beta vulgaris L.), apples (Malus domestica Borkh.), bananas (Musa paradisiaca), common beans (Phaseolus vulgaris), oranges (Citrus sinensis (L.) Osbeck), onions (Allium cepa L.), and lettuce (Lactuca sativa L.). Other foods also showed antioxidant capacity. The binomial antioxidant capacity of foods and health was extensively discussed according to science literature. Based on the high TAC content of Brazil nuts, guaraná, coffee, chocolate, collard greens, apples, beets, beans, oranges, onions and other foods, their regular dietary intake is strongly recommended to reduce the risk of chronic non-communicable diseases.

  9. A Missão do Tradutor - Aspectos da Concepção Benjaminiana de Linguagem e Tradução

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    Mauri Furlan

    1996-01-01

    Full Text Available MUITO ALÉM DO PRECONCEITO DE TRAIÇÃO que tradicionalmente possa evocar, a tradução, no pensamento benjaminiano, ressurge excelsa,com uma função redentora. Na hipótese algo platônica e místico-religiosa da existência de uma "língua pura" (reine Sprache, imaterial, supra-sensível, da qual todas as línguas são reflexo, encontra-se apossibilidade real da tradução. Walter Benjamin, em seu famoso textosobre tradução, A Tarefa do Tradutor, define tradução como "forma",esclarecendo-a também frente a outras definições negativas: traduçãonão é recepção, não é comunicação, não é imitação.

  10. Effects of sulfur dioxide on conifers

    Energy Technology Data Exchange (ETDEWEB)

    Govi, G.; Tagliani, F.; Cimino, A.

    1974-01-01

    Trials on the resistance of several conifer and oak species to the effects of sulfur dioxide at different concentrations and moisture levels were conducted. 72 combinations were experimented. The damages began to appear under the following conditions: Abies alba: 0.3 ppm, 25/sup 0/C, 70% ur after 24 hours; Picea excelsa: 0.3 ppm, 15/sup 0/C, 70-95% ur after 24 hours; Cedrus deodara: 0.3 ppm, 15/sup 0/C, 95% ur after 48 hours; Pinus pinea: 0.3 ppm, 15/sup 0/C, 70-95% after 72 hours; Pinus strobus 0.3 ppm, 25/sup 0/C, 70-95%, after 48 hours; Pinus pinaster: similar to the former; Pinus nigra: 2 ppm, 25/sup 0/C, 70-95%, ur after 5 days; Cupressus arizonica and C. semperivirens: 2 ppm, 25%/sup 0/C, 90% ur after 72 hours; Quercus robur: 5 ppm, 25/sup 0/C, 90% ur, after 10 days. 6 references, 3 figures, 1 table.

  11. Origin and Domestication of Native Amazonian Crops

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    Doriane Picanço-Rodrigues

    2010-01-01

    Full Text Available Molecular analyses are providing new elements to decipher the origin, domestication and dispersal of native Amazonian crops in an expanding archaeological context. Solid molecular data are available for manioc (Manihot esculenta, cacao (Theobroma cacao, pineapple (Ananas comosus, peach palm (Bactris gasipaes and guaraná (Paullinia cupana, while hot peppers (Capsicum spp., inga (Inga edulis, Brazil nut (Bertholletia excelsa and cupuassu (Theobroma grandiflorum are being studied. Emergent patterns include the relationships among domestication, antiquity (terminal Pleistocene to early Holocene, origin in the periphery, ample pre-Columbian dispersal and clear phylogeographic population structure for manioc, pineapple, peach palm and, perhaps, Capsicum peppers. Cacao represents the special case of an Amazonian species possibly brought into domestication in Mesoamerica, but close scrutiny of molecular data suggests that it may also have some incipiently domesticated populations in Amazonia. Another pattern includes the relationships among species with incipiently domesticated populations or very recently domesticated populations, rapid pre- or post-conquest dispersal and lack of phylogeographic population structure, e.g., Brazil nut, cupuassu and guaraná. These patterns contrast the peripheral origin of most species with domesticated populations with the subsequent concentration of their genetic resources in the center of the basin, along the major white water rivers where high pre-conquest population densities developed. Additional molecular genetic analyses on these and other species will allow better examination of these processes and will enable us to relate them to other historical ecological patterns in Amazonia.

  12. Antileukemic Effect of Tualang Honey on Acute and Chronic Leukemia Cell Lines

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    Nik Muhd Khuzaimi Nik Man

    2015-01-01

    Full Text Available Complementary medicine using natural product as antitumor is on the rise. Much research has been performed on Tualang Honey and it was shown to have therapeutic potential in wound healing, and antimicrobial activity and be antiproliferative against several cancer models such as human osteosarcoma (HOS, human breast (MCF-7 and MDA-MB-231, and cervical (HeLa cancer cell lines. To date, there was limited study on antileukemic properties of Tualang (Koompassia excelsa Honey. The aim of this study was to evaluate the antileukemic effect of Tualang Honey on acute and chronic leukemia cell lines. Leukemia cell lines (K562 and MV4-11 and human mononuclear cell isolated from peripheral blood were grown in RPM1 1640 culture medium. The cells were incubated with increasing concentrations of Tualang Honey. After incubation, the evaluation of viability and apoptosis was performed. The morphological changes of leukemia cells were the presence of cytoplasmic blebs followed by apoptotic bodies and round shape of cells. IC50 against K562 and MV4-11 was determined. Tualang Honey gave 53.9% and 50.6% apoptosis activity on K562 and MV4-11, respectively, while on human mononuclear cell it was 37.4%. Tualang Honey has the apoptosis-inducing ability for acute and chronic myeloid leukemia (K562 and MV4-11 cell lines.

  13. Natural variation of selenium in Brazil nuts and soils from the Amazon region.

    Science.gov (United States)

    Silva Junior, E C; Wadt, L H O; Silva, K E; Lima, R M B; Batista, K D; Guedes, M C; Carvalho, G S; Carvalho, T S; Reis, A R; Lopes, G; Guilherme, L R G

    2017-12-01

    Brazil nut tree (Bertholletia excelsa) is native of the Amazon rainforest. Brazil nuts are consumed worldwide and are known as the richest food source of selenium (Se). Yet, the reasoning for such Se contents is not well stablished. We evaluated the variation in Se concentration of Brazil nuts from Brazilian Amazon basin, as well as soil properties, including total Se concentration, of the soils sampled directly underneath the trees crown, aiming to investigate which soil properties influence Se accumulation in the nuts. The median Se concentration in Brazil nuts varied from 2.07 mg kg - 1 (in Mato Grosso state) to 68.15 mg kg - 1 (in Amazonas state). Therefore, depending on its origin, a single Brazil nut could provide from 11% (in the Mato Grosso state) up to 288% (in the Amazonas state) of the daily Se requirement for an adult man (70 μg). The total Se concentration in the soil also varied considerably, ranging from Brazil nuts generally increased in soils with higher total Se content, but decreased under acidic conditions in the soil. This indicates that, besides total soil Se concentration, soil acidity plays a major role in Se uptake by Brazil nut trees, possibly due to the importance of this soil property to Se retention in the soil. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Effects of electron-beam irradiation on HDPE/Brazil nut shell fiber composite

    International Nuclear Information System (INIS)

    Ferreira, Maiara S.; Sartori, Mariana N.; Oliveira, Rene R.; Moura, Esperidiana A.B.

    2013-01-01

    In recent years, research on the replacement of synthetic fibers by natural fibers as reinforcement in thermoplastic composites has increased dramatically due to the advantages of natural fibers, such as low density, low cost, environmental appeal and recyclability. In the present work, the influence of electron-beam irradiation on mechanical properties of HDPE and HDPE/Brazil Nut Shell (Bertholletia excelsa) fiber compositive was investigated. The HDPE composite reinforced with 5% or 10%, by weight of Brazil nut shell fiber powder with particle sizes equal or smaller than 250 μm were obtained by extrusion, using a twin screw extruder. The materials were irradiated at 200 kGy using a 1.5 MeV electron beam accelerator, at room temperature in presence of air. The irradiated and non-irradiated specimens tests samples were submitted to mechanical and thermo-mechanical tests, scanning electron microscopy (SEM), X-Ray diffraction (XRD) and sol-gel analysis and the correlation between their properties was discussed. The results showed significant changes in HDPE mechanical and thermo-mechanical properties due to Brazil nut shell fibers addition and electron-beam irradiation. The surface of the cryo fractured composite samples irradiated showed important visual changes which suggest a better fiber-matrix interfacial adhesion, due to irradiation treatment. These results showed that it is possible to get interesting property gains by using waste from renewable sources instead of the traditional ones and electron-beam radiation treatment. (author)

  15. Efeitos dos estresses hidrico e salino e da ação de giberelina em sementes de Senna spectabilis

    Directory of Open Access Journals (Sweden)

    Helma Jeller

    2001-01-01

    Full Text Available Senna spectabilis (DC. Irwin et Barn. variedade excelsa (Schrad. Irwin et Barn. is a leguminosae woody species of low frequency in cerrado and caatinga of the Brazilian northeast. It is used in landscaping designs due to it’s natural beauty, streets arborization and in reflorestation of degraded areas. The aim of this work was to extend the knowledge about the biology of this species and to study the effects of water and salt stress and gibberellins on seed germination. Four replications of 50 seeds were used for each treatment and germination was carried out in 9cm Petri lived with filter paper moistened with test-solution. Senna espectabilis seeds did not present a wide tolerance to water stress and the germination was supressed at -0,7MPa. The reduction of NaCl osmotic potential decreased germination and synchrony of the process. The maximal tolerance limit was at -1,6MPa, when germination was totally inhibited. The addition of gibberellic acid reduced partially the effects of water and salt stress.

  16. Davras Dagı (Isparta ve Çevresinin Orman ve Çalı Vejetasyonu

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    Coşkun SAĞLAM

    2009-04-01

    Full Text Available Bu vejetasyon çalısması, 2002-2005 yılları arasında Batı Toroslar'da yer alan Davras Dagı ve çevresinde gerçeklestirildi. Vejetasyon Braun-Blanquet (1964 metoduna göre analiz edilerek orman ve çalı vejetasyonuna ait yedi birlik tespit edildi. Bunlardan bes tanesi yenidir. Orman vejetasyonu: Sınıf : Quercetea pubescentis (Oberd, 1948 Doing Kraft, 1955 Ordo : Querco – Cedretalia libani Barbéro, Loisel ve Quézel, 1974 1. Minuartio globulosi – Juniperetum excelsae ass. nova 2. Sileno squamigeri – Quercetum cerridis ass. nova 3. Astragalo oxytropifolii – Pinetum caramanicae ass. nova Alyans : Lonicero nummulariaefoliae – Cedrion libani Quézel, Barbéro ve Akman 1978 4. Veronico syriaci – Cedretum libani ass. nova 5. Diantho cibrarii – Quercetum vulcanicae Kurt et al. 1996 Sınıf : Quercetea ilicis Br.-Bl., 1947 Ordo : Quercetalia ilicis Br.-Bl., 1947, Rivaz Martinez, 1974 Alyans : Quercion cocciferae Quézel, Barbéro, Akman, 1978 6. Hyperico polyphylli – Pinetum brutiae ass. nova Çalı Vejetasyonu: Sınıf : Quercetea pubescentis (Oberd, 1948 Doing Kraft, 1955 Ordo : Querco – Cedretalia libani Barbéro, Loisel ve Quézel, 1974 7. Astragalo atropurpureus– Quercetum cocciferae Kargıoglu, 19

  17. Notas biográficas y pedagógicas sobre el padre Feijoo

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    Valentín Martínez-Otero Pérez

    2017-07-01

    Full Text Available El benedictino español Benito Feijoo (1676-1764 fue uno de los intelectuales más destacados del siglo XVIII. Sus aportaciones al mundo del pensamiento y la cultura abarcan un buen número de ensayos sobre los saberes eruditos y populares de la época. En lo que se refiere al gran alcance pedagógico de Feijoo, se advierte a un tiempo en su condición de profesor universitario, en su compromiso crítico y empírico con la eliminación de errores y en su actitud crítica y empirista, que le lleva a poner en entredicho obsoletos métodos de enseñanza. La obra del benedictino, aunque es ensayística y carece de sistematización, tiene una finalidad educativa. Este artículo, tras repasar algunos datos biográficos, se centra en algunas de sus contribuciones pedagógicas más destacadas, como el cultivo del entendimiento y la defensa de las mujeres. En definitiva, Feijoo es un iniciador, un educador de gran relevancia histórica que, en el marco de su elevada misión pedagógica, logró agitar las conciencias. Por ello, su excelsa figura continúa brillando y se sigue estudiando en este siglo XXI.

  18. Vegetative rhythm of some woody species

    International Nuclear Information System (INIS)

    Gagnaire, J.

    1965-01-01

    In laboratory conditions from march 1963 to april 1964, variations of calcium absorption by roots and translocation speed towards aerial parts of young norway spruce (Picea excelsa) in hydroponic growing, present fixed stages in a vegetative cycle: 1. from early april until end may a rapid rising of minerals from roots to young parts of the tree, associated with a redistribution of calcium formerly fixed in tissues; 2. in June-July, an equilibrium in mineral exchanges between the different parts of the tree; 3. from early august to end of October, a pre-dormancy phase marked by a slowing of translocation; 4. in november-december, an absolute dormancy period; 5. from January to march, a post-dormancy phase in which root absorption and translocation to aerial parts recover. In outdoors conditions and for a determined climate these stages start and end on dates linked to species and variety: - in Grenoble, absolute dormancy starts in September for thuyas, early October for maple-trees, middle October for norway spruce and novembers for poplars. (authors) [fr

  19. A POLICY MIX TO PREVENT A NON-COMMONS TRAGEDY FOR COLLECTIVE FOREST RESERVES IN AGRARIAN SETTLEMENTS IN NORTHWEST MATO GROSSO

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    R.B. Davenport

    Full Text Available ABSTRACT Agrarian reform settlements have recently been identified as a major contributor to ongoing deforestation in the Brazilian Amazon. Collective forest reserves were established in some settlements to thwart forest loss, but settlers usually do not recognize forest commons, opting instead for continued private accumulation through forest clearing, or a "non-commons" tragedy. Pathways toward ensuring the viability of common-pool management in settlements remain unclear. Our case study focused on two similarly sized settlements in neighboring municipalities in Northwest Mato Grosso, Brazil, each with formally designated collective forest areas. In one, deforestation shifted into the collective reserve and intensified; in the other, deforestation stabilized and the collective reserve was protected. In the latter, settlers understood their collective forest reserve as a commonly held asset, with two commercially viable settlement cooperatives involved in Brazil nut (Bertholletia excelsa extractivism and added value processing. We analyze differences between the two settlements using Elinor Ostrom's framework for analysis of socio-ecological systems, and identify the locally applied policy mix ensuring the viability of a forest commons. The study concludes that "top-down" efforts to institutionalize collective governance over remaining forests will likely fail in most settlements, without a focus on alternative livelihood opportunities synergetic with forest tenure.

  20. FOREST SPECIES EVALUATION IN THE FOREST REGION IN RORAIMA STATE

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    Helio Tonini

    2006-03-01

    Full Text Available This study was carried out to evaluate 19 native and exotic species and to identify potential species for plantation inhomogeneous stands and in agroforestry systems in forest ecosystem in Roraima State. The species were planted from 1997 to 2000.When the objective was sawn wood production the spacing was 4 x 3 m and when the objective was energy and pulp production thespacing was 3 x 2 m. The samples consisted of 9 lines of 9 plants, totaling 81 plants, coming down to 49 useful trees. In each useful treewere measured the DBH (diameter at 1.30 m and total height. The merchantable volume per hectare was obtained with themerchantable form factor determination, through the scaling of two medium trees using the Smalian method. Each species, was alsoevaluated in relation to survival, bole quality, phytossanitary aspects and wind damages.The data analysis indicated as promisingspecies for use in energy, Eucalyptus urograndis; Schizolobium amazonicum as low or medium wood density species for use inveneer production and Bertholettia excelsa as medium to high density for use in sawn timber.

  1. ADDITIONAL NOTE ON CULLENIA CEYLANICA K. Schum. (Bombac

    Directory of Open Access Journals (Sweden)

    A. J. G. H. KOSTERMANS

    2015-11-01

    Full Text Available Cullenia excelsa Wight in Pearson & Brown, Commerc. Timb. India 1:141, t. 51 1932; Foxworthy in Philipp. J. Sci. Bot. 4: 500. 1909; Trotter, Common Comm. Timb. India 65. 1901. Foxworthy in Philip. J. Sci. Bot. 4: 500. 1909.Fruit collected in January 1958 from the tree cultivated in the BogorBotanical Garden (no. XVI H. 13 enables me to correct and emendatethe description published in "Communication of the Forest ResearchInstitute, Bogor" no. 51 (1956 and in Reinwardtia 4 : 69.1956.The ripe fruit are dirty green outside, they open on the branches andthe seed falls out immediately.Each fruit contains from one to 5 seeds, the common number, however,is one, the others do not develop; the fruit becomes pseudo-unilocular.The chestnut brown seeds are completely enveloped by a lightbrown,somewhat glassy, slightly juicy and hardly sweet, furrowed aril of 2—4mm thickness; the apical part of the aril is more or less fimbriate. —Figs. 38, 39.

  2. Effects of electron-beam irradiation on HDPE/Brazil nut shell fiber composite

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Maiara S.; Sartori, Mariana N.; Oliveira, Rene R.; Moura, Esperidiana A.B., E-mail: maiara.sferreira@gmail.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    In recent years, research on the replacement of synthetic fibers by natural fibers as reinforcement in thermoplastic composites has increased dramatically due to the advantages of natural fibers, such as low density, low cost, environmental appeal and recyclability. In the present work, the influence of electron-beam irradiation on mechanical properties of HDPE and HDPE/Brazil Nut Shell (Bertholletia excelsa) fiber compositive was investigated. The HDPE composite reinforced with 5% or 10%, by weight of Brazil nut shell fiber powder with particle sizes equal or smaller than 250 μm were obtained by extrusion, using a twin screw extruder. The materials were irradiated at 200 kGy using a 1.5 MeV electron beam accelerator, at room temperature in presence of air. The irradiated and non-irradiated specimens tests samples were submitted to mechanical and thermo-mechanical tests, scanning electron microscopy (SEM), X-Ray diffraction (XRD) and sol-gel analysis and the correlation between their properties was discussed. The results showed significant changes in HDPE mechanical and thermo-mechanical properties due to Brazil nut shell fibers addition and electron-beam irradiation. The surface of the cryo fractured composite samples irradiated showed important visual changes which suggest a better fiber-matrix interfacial adhesion, due to irradiation treatment. These results showed that it is possible to get interesting property gains by using waste from renewable sources instead of the traditional ones and electron-beam radiation treatment. (author)

  3. New, rare or remarkable microfungi in the Italian Alps (Carnic Alps)--part I--ascomycotina.

    Science.gov (United States)

    Feige, G B; Ale-Agha, N; Jensen, M; Christiaans, B; Kricke, R

    2004-01-01

    During our observations in the SE part of the Carnic Alps in the year 2003 we were able to collect and identify 35 ascomycetes on trees and dead wood. Among these one can find numerous ascomycetes of different orders e.g. Pyrenomycetes, Loculoascomycetes and Discomycetes. Some species like Botryosphaeria ribis GROSENLUCHER & DUGGAR on Ribes alpinum L., Dothiora pyrenophora (FR.) FR. on Sorbus aucuparia L., Gemmamyces piceae (BORTH.) CASAGO. on Picea excelsa (LAM.) LINK, Glomerella montana (SACC.) v. ARX & E. MULLER on Sesleria caerulea (L.) ARD, Hymenoscyphus immutabilis (Fuck.) Dennis on Alnus incana (L.) Moench, Hysterographium fraxini (PERS. Ex. FR.) de Not. on Fraxinus ornus L., Lachnellula willkommii (Hartig) DENNIS [= Trichascyphella willkommii (Hartig) NANNF.] on Larix decidua MILL.,Leptosphaeria lycopodina (Mont.) SACC. on Lycopodium annotinum L., Mollisia adenostylidis REHM. on Adenostyles glabra (MILL.) DC., Pezicula cinnamomea (DC.)SACC. [ana: Cryptosporiopsis quercina PETRAK] on Quercus robur L., Pyrenopeziza petiolaris (A. & S. Ex FR.) NANNF. on Acer pseudoplatanus L., Tapesia rosae (PERS.) FUCKEL on Rosa canina L., are new for this area. All specimen are deposited in the Herbarium ESS Mycotheca Parva, Collection G.B. Feige/N. Ale-Agha.

  4. Floristic and structural analysis of premontane humid forests in Amalfi (Antioquia, Colombia)

    International Nuclear Information System (INIS)

    Ariza Cortes, William; Toro Murillo, Juan Lazaro; Lores Medina, Angelica

    2009-01-01

    The floristic composition and the structure of a humid hill forest were determined. The forest is located in the northernmost area of the Colombian Central Mountain Range. The methodology proposed by ISA-JAUM was employed in this study, from a 0.1 ha sample. Moreover, plant material was collected from clear spots, stubbles and grasslands. A total of 421 vascular plants species were found. Two hundred and thirty eight of these come from the 0.1 ha sample and only 150 had DBH> 2.5 cm, which highlights the benefits of the sample method employed, since it was possible to record a large number of epiphytic and herbaceous elements in the forest. In general, the composition matches what was previously reported in similar areas. The families Melastomataceae (32), Lauraceae (31), Rubiaceae (29) and Araceae (15) were the ones holding the largest number of species. New chorological records for the state of Antioquia were reported in this study; for instance, Colombobalanus excelsa (Fagaceae). High density of individuals (388) was found at structural level. The patterns of height and diametrical classes followed the typical distribution of disetaneous tropical forests, in which the largest amount of individuals occur in the lowest classes and few species with individuals having the greatest diameters and heights account for the major ecological weight in the forest. Despite the great diversity documented, these forests are currently subject to an intensive process of fragmentation and loss of coverage.

  5. THE GENUS CULLENIA Wight * (Bombacaceae

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    A. J. G. H. KOSTERMANS

    1956-12-01

    Full Text Available The monotypic genus Cullenia was established by Wight (IconesPI. Ind. or. 5 (1 : pi. 1761—62 & text, 1851, who differentiated it fromDurio Adans. mainly by the lack of a corolla and the position and shapeof the anthers. The only species, originally described as Durio ceylanicusby Gardner, was cited by Wight as Cullenia excelsa Wight. K. Schumanncorrected the specific epithet rather casually and atributed it (wronglyto Wight. Bentham (in Benth. & Hook., Gen. pi. 1: 212. 1867; Baillon(Hist. pi. 4: 159. 1872, Masters (in Hook, f., Fl. Br. Ind. 1: 350. 1874and Beccari (Malesia 3: 219. 1889 accepted the genus.Bakhuizen van den Brink (in Bull. Jard. bot. Buitenzorg III, 6: 228.1924 incorporated the genus in Durio.In my opinion Cullenia represents a "good" genus by its lack ofcorolla. Alston, although accepting Bakhuizen's reduction, informed mepersonally, that he, too, is inclined to consider Cullenia different fromDurio.The pollen were described as being naked and pedicellate by Gardner;this wrong statement was corrected by Wight; the anthers are pedicellateand one-celled.In this paper a new Cullenia species is described, which strengthensthe position of the genus; both species are restricted to the rain forestregion of Ceylon and the Southern Indian Peninsula.

  6. Spatial pattern of Amazonian timber species using cartesian and spatial coordinates method

    Directory of Open Access Journals (Sweden)

    Tiago Monteiro Condé

    2016-06-01

    Full Text Available Geographic information system (GIS applied to forest analysis permit the recognition and analysis of spatial patterns of species in two and three dimensional. The aim of this study to demonstrate the efficiency of cartesian and spatial coordinates method (MCCE, method of correcting UTM coordinates of trees location in accordance with the location of field or Cartesian (X ,Y, combined with natural neighbor index (ANND in recognition and analysis of spatial distribution patterns of four commercial timber species in forest management in Caracaraí, Roraima State, Brazil. Simulations were performed on 9 ha, divided into 100 plots of 100 m2 each. Collected data were DBH > 10 cm, commercial and total heights, cartesian coordinates (X,Y and spatial coordinates (UTM. Random spatial patterns were observed in Eschweilera bracteosa and Manilkara huberi. The dispersed and rare spatial patterns were observed in Dinizia excelsa and Cedrelinga cateniformis. MCCE proved to be an efficient method in the recognition and analysis of spatial patterns of native species from Amazon rain forest, as forest planning becomes easier by 2D and 3D simulations.

  7. Effect of Oxygen-Reducing Atmospheres on the Safety of Packaged Shelled Brazil Nuts during Storage

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    Vildes Maria Scussel

    2011-01-01

    Full Text Available This work reports the application of oxygen-(O2- reducing atmosphere methods on stored shelled Brazil nut (Bertholletia excelsa H.B.K. packs aiming to evaluate the degree of aflatoxin degradation, nuts lipid oxidative stability, fungi control, and hygienic conditions improvement. The methods applied were (a ozone: O3, (b carbon dioxide: CO2, and (c O2 absorber pads with and without vacuum. From all modified atmospheres evaluated, the best performance was obtained with O3, either with or without vacuum. It was the only nut treatment that was able to degrade aflatoxins. None of the spiked (AFLs: 15 μg·kg−1 nut samples O3- treated had aflatoxins detected up to the LC-MS/MS method LOQ (0.36 μg·kg−1 for total AFLs, thus producing safer nuts. Also it kept the fatty acid oxidation indicator—malondialdehyde stable and improved the sensory attributes for consumer acceptance. In addition, the destruction of fungi and yeast was observed since the O3 application (from 1.8×104 cfu/g to NG = no growth. All other treatments stabilized and/or inhibited microorganisms' growth only. By adding CO2 gas also played an important role in the nut quality. Regarding cost, gaseous O3 showed to be of low cost for application in the nut packs.

  8. Analysis of IgE binding proteins of mesquite (Prosopis juliflora) pollen and cross-reactivity with predominant tree pollens.

    Science.gov (United States)

    Dhyani, Anamika; Arora, Naveen; Gaur, Shailendra N; Jain, Vikram K; Sridhara, Susheela; Singh, Bhanu P

    2006-01-01

    Pollen from the mesquite tree, Prosopis juliflora, is an important source of respiratory allergy in tropical countries. Our aim was to partially characterize the IgE binding proteins of P. juliflora pollen extract and study cross-reactivity with prevalent tree pollen allergens. Intradermal tests with P. juliflora and five other tree pollen extracts were performed on respiratory allergy patients from Bikaner (arid) and Delhi (semi arid). Prosopis extract elicited positive skin reactions in 71/220 of the patients. Sera were collected from 38 of these 71 patients and all demonstrated elevated specific IgE to P. juliflora. Immunoblotting with pooled patients' sera demonstrated 16 IgE binding components, with components of 24, 26, 29, 31, 35, 52, 58, 66 and 95 kDa recognized by more than 80% of individual patients' sera. P. juliflora extract is allergenically potent requiring 73 ng of self-protein for 50% inhibition of IgE binding in ELISA inhibition. Cross-inhibition assays showed close relationship among P. juliflora, Ailanthus excelsa, Cassia siamea and Salvadora persica. IgE binding components of 14, 41, 52 and 66 kDa were shared allergens whereas 26 and 29 kDa were specific to P. juliflora. The findings suggest that purification of cross-reactive allergens will be helpful for diagnosis and immunotherapy of tree pollen allergic patients.

  9. A clinically relevant major cross-reactive allergen from mesquite tree pollen.

    Science.gov (United States)

    Dhyani, A; Singh, B P; Arora, N; Jain, V K; Sridhara, S

    2008-10-01

    Prosopis juliflora (mesquite) is one of the major sources of pollinosis in tropical and semi-arid countries of the world. The present study was undertaken to purify and characterize a major cross-reactive allergen from this tree species. Mesquite pollen extract was purified using reverse-phase chromatography. Allergen characterization was done by electrophoresis, enzyme-linked immunosorbent assay (ELISA) and Western blotting. Clinical relevance of the purified protein was analyzed by in vivo (skin tests) and in vitro experiments such as ELISA, histamine release, peripheral blood mononuclear cells (PBMC) proliferation and cytokine assays. Cross-reactivity of purified protein with allergenic tree species and lima bean (food) was assessed by inhibition assays. A 66-kDa protein was purified from mesquite pollen extract using octadecyl silica resin. Purified protein recognized 90% of mesquite-sensitized patients in skin test and ELISA. It induced significant histamine release in allergic patients' blood and interleukin-4 secretion in the PBMC culture supernatants. Inhibition assays suggested close allergenic relationship of this protein with Ailanthus excelsa, Cassia siamea, Salvadora persica pollen and Phaseolus lunatus (lima bean - an edible legume). A 66-kDa major cross-reactive allergen was isolated from mesquite pollen using single-step purification procedure. The protein seems relevant for clinical applications in allergic disorders.

  10. Non-destructive methods for peat layer assessment in oligotrophic peat bogs: a case study from Poiana Ştampei, Romania

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    Iuliana F. Gheorghe

    2011-01-01

    Full Text Available Practices currently employed in the investigation and characterisation of peat deposits are destructive and may irremediable perturb peat bog development even in cases when exploitation is not carried out. We investigated the correlation between vegetation characteristics in the active area of Poiana Ştampei peat bog, Romania, and the underlying peat layer depth, aiming at establishing a non-destructive method of peat layer depth estimation. The presence of the Sphagneto-Eriophoretum vaginati association, dominated by Sphagnum fimbriatum, Eriophorum vaginatum, Andromeda polifolia, Vaccinium oxycoccos, V. myrtillus, V. vitis-idaea, Polytrichum commune, Picea excelsa, Pinus sylvestris and Betula verrucosa was found to predict the existence of the peat layer but not its depth. Out of the seven identified vegetation types, one type was associated with a very thin or no peat layer, one type was characterised by the presence of a thick (over 100 cm peat layer and five types indicated the presence of variable average depths of the peat layer. pH values correlated with peat layer depth only within the vegetation type associated with thick peat layers.

  11. GROWTH OF AMAZON NATIVE SPECIES SUBMITTED TO THE PLANTATION IN THE RORAIMA STATE

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    Helio Tonini

    2009-10-01

    Full Text Available An important forest research challenge in the Amazonian is finding forms of reforesting degraded areas with the use of a larger number of native species and identify tropical species commercially attractive adapted to clear-cut areas. This work had as objectives to evaluate the initial growth in diameter and height of six native Amazonian species in a preliminary species trial. The data were obtained from measures of 72 trees 9 years after planting. The selected species for this study were cupiúba (Goupia glabra, cumaru (Dipterix odorata, andiroba (Carapa guianensis, brazil nut (Bertholletia excelsa, pará-pará (Jacaranda copaia and tatajuba (Bagassa guianensis. The cumulative growth curves for diameter and height was obtained by the Chapman – Richards function. In spite of the low age of the stands, it was obtained good fit to the function for the studied species. Pará-pará (Jacaranda copaia, presented best diameter and height growth in all the ages. The diameter mean annual increment analysis showed that, except for the cupiúba (Goupia glabra,  can be expected  increments larger than  2 cm, by appropiate spacings and thinning.

  12. Crescimento de espécies nativas da Amazônia submetidas ao plantio no estado de Roraima

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    Helio Tonini

    2008-01-01

    Full Text Available An important forest research challenge in the Amazonian is finding forms of reforesting degraded areas with the use of a larger number of native species and identify tropical species commercially attractive adapted to clear-cut areas. This work had as objectives to evaluate the initial growth in diameter and height of six native Amazonian species in a preliminary species trial. The data were obtained from measures of 72 trees 9 years after planting. The selected species for this study were cupiúba (Goupia glabra, cumaru (Dipterix odorata, andiroba (Carapa guianensis, brazil nut (Bertholletia excelsa, pará-pará (Jacaranda copaia and tatajuba (Bagassa guianensis. The cumulative growth curves for diameter and height was obtained by the Chapman – Richards function. In spite of the low age of the stands, it was obtained good fit to the function for the studied species. Pará-pará (Jacaranda copaia, presented best diameter and height growth in all the ages. The diameter mean annual increment analysis showed that, except for the cupiúba (Goupia glabra, can be expected increments larger than 2 cm, by appropiate spacings and thinning.

  13. AMAZON RAINFOREST COSMETICS: CHEMICAL APPROACH FOR QUALITY CONTROL

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    Mariko Funasaki

    2016-02-01

    Full Text Available The market for natural cosmetics featuring ingredients derived from Amazon natural resources is growing worldwide. However, there is neither enough scientific basis nor quality control of these ingredients. This paper is an account of the chemical constituents and their biological activities of fourteen Amazonian species used in cosmetic industry, including açaí (Euterpe oleracea, andiroba (Carapa guianensis, bacuri (Platonia insignis, Brazil nut (Bertholletia excelsa, buriti (Mauritia vinifera or M. flexuosa, cumaru (Dipteryx odorata, cupuaçu (Theobroma grandiflorum, guarana (Paullinia cupana, mulateiro (Calycophyllum spruceanum, murumuru (Astrocaryum murumuru, patawa (Oenocarpus bataua or Jessenia bataua, pracaxi (Pentaclethra macroloba, rosewood (Aniba rosaeodora, and ucuuba (Virola sebifera. Based on the reviewed articles, we selected chemical markers for the quality control purpose and evaluated analytical methods. Even though chromatographic and spectroscopic methods are major analytical techniques in the studies of these species, molecular approaches will also be important as used in food and medicine traceability. Only a little phytochemical study is available about most of the Amazonian species and some species such as açaí and andiroba have many reports on chemical constituents, but studies on biological activities of isolated compounds and sampling with geographical variation are limited.

  14. Fatty acids profile of pulp and nuts of Brazilian fruits

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    Paulo Afonso da Costa

    2011-12-01

    Full Text Available Fruits and nuts from the North and Northeast regions of Brazil were collected to determine the fatty acid profile of their oils. The species studied were Brazil (Bertholletia excelsa H.B.K., Mucajá (Couma rigida M., Inajá (Maximiliana maripa D., Jenipapo (Genipa Americana L., and Buriti (Mauritia flexuosa L. nuts. Fatty acid methyl esters were analyzed by gas chromatography with flame ionization detection (GC-FID. Brazil nut major fatty acid was 18:3n-3 (α-linolenic acid, and Buriti nut had approximately 23 times more 18:3n-3 than the pulp. Mucajá nut presented high content of 12:0 (lauric acid and 16:0 (palmitic acid, and Mucajá pulp showed significant levels of 18:2n-6 (linoleic acid. Considering the PUFA (polyunsaturated fatty acid sum values, almost all fruits and nuts analyzed presented very high levels of these compounds. Regarding n-6/n-3 ratio, only Brazil Nut, Buriti Nut, Inajá pulp, and Jenipapo pulp corresponded to the desired profile. These Brazilian fruits and nuts could be of potential interest due to their high nutritive value and lipid content.

  15. Arborização viária urbana: espécies de pequeno porte com potencial de uso.

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    Luzia Ferreira Da Silva

    2005-06-01

    Full Text Available A escolha de espécies apropriadas é um dos pontos mais importantes para os profissionais que trabalham com arborização viária urbana. Com esse objetivo desenvolveu-se um estudo no Arboreto do Instituto Agronômico de Campinas, onde foram observadas a morfologia e a fenologia de 22 espécies no período de outubro de 2000 a outubro de 2002, num total de 66 indivíduos que apresentavam tronco sem espinhos e copas arredondadas ou ovaladas. As fenofases analisadas foram floração, frutificação, brotação e abscisão foliar. A maioria apresentou floração longa (mais de quatro meses com flores vistosas no período primavera-verão. Cordia sebestena, Kielmeyera excelsa, Saraca indica, Lonchocarpus filipes, Metternichia princeps estão entre as que apresentaram grande potencial, por serem as que mais se aproximaram ao padrão desejado para a arborização viária urbana.

  16. A quest for staunch effects of flavonoids: Utopian protection against hepatic ailments

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    Anju Dhiman

    2016-11-01

    Full Text Available The role of flavonoids as the major red, blue and purple pigments in plants has gained these secondary products a great deal of attention over the years. Flavonoids are polyphenols and occur as aglycones, glycosides and methylated derivatives. Flavonoids are the main components of a healthy diet containing fruits and vegetables and are concentrated especially in tea, apples and onions. Till date, more than 6000 flavonoids have been discovered, out of which 500 are found in free state. They are abundant in polygonaceae, rutaceae, leguminosae, umbelliferae and compositae. Flavonoids are powerful antioxidants. In addition to their role in nutrition, flavonoids possess many types of pharmacological activities, including anti-inflammatory, antioxidative, hepatoprotective, vasorelaxant, antiviral and anticarcinogenic effects. The present review is focused on flavonoids derived from natural products that have shown a wise way to get a true and potentially rich source of drug candidates against liver ailments. The present review initially highlights the current status of flavonoids and their pharmaceutical significance, role of flavonoids in hepatoprotection, therapeutic options available in herbal medicines and in later section, summarizes flavonoids as lead molecules, which have shown significant hepatoprotective activities.

  17. Methodology to evaluate the insecticide potential of forest tree species

    International Nuclear Information System (INIS)

    Morales Soto, Leon; Garcia P, Carlos Mario

    2000-01-01

    The flora diversity of Colombia has an enormous potential in the rational use of its forest resources. Trees with biocidal effects to control pests and diseases need to be investigated. The objective of this research was to develop a methodology with low costs, easy application and quick results. The methodology employed was as follows: selection of tree species based on bibliography, ancestral reports and personal observations. The process was as follows: field collection of plants, preparation of plants extracts and test with Artemia salina Leach to detect biological activity of the extracts using LC50. Bioassays with those extract more promising (LC50 less than 1000 ppm) Determination of active compounds. The methodology was employed with 5 forest tree species: guarea guidonia (L) Sleumer and trichia hirta L. (Meliaceae), Machaerium Moritzianum Benth. (Fabaceae), Swinglea glutinosa Merrill (rutaceae) and Mammea americana L. (Clusiaceae). Using Artemia salina Leach as indicator of biocidal potential, two species were selected as the most promising, those were: Swinglea glutinosa Merril and Machaerium moritzianum Benth. In addition bioassays were made to evaluate fagoinhibition on Atta cephalotes (L.) (Hym: Formicidae) and control of Alconeura. This methodology is recommended for this kind of research

  18. Methodology for the insecticide potential evaluation of forest species

    International Nuclear Information System (INIS)

    Morales Soto, Leon; Garcia P, Carlos Mario

    2000-01-01

    The flora diversity of Colombia has an enormous potential in the rational use of its forest resources. Trees with biocides effects to pest control and diseases need to be investigated. The objective of this research was to develop a methodology with low costs, easy application and quick results. The methodology employed was as follows: selection of tree species based on bibliography, ancestral reports and personal observations. The process was as follows: field collection of plants, preparation of plants extracts, and test with Artemia Salina, leach to detect biological activity of the extracts using LC50. Bioassays with those extract more promising (LC50 less than 1000 ppm). The methodology was employed with 5 forest tree species: Guarea guidonia (L.) Sleumer and Trichia hirta L. (Meliaceae), machaerium moritzianum Benth. (Fabaceae), Swinglea glutinosa Merrill. (Rutaceae) and Mammea americana L. (Clusiaceae). Using Artemia salina Leach as indicator of biocidal potential, two species were selected as the most promising, those were: Swinglea glutinosa Merril and Machaerium moritzianum Benth. In addition bioassays were made to evaluate fagoinhibition on Atta cephalotes (L.) and control of Alconeura. This methodology is recommended for this kind of research

  19. Growth ring analysis of Euxylophora paraensis through x-ray microdensitometry

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    Eva Santos de Andrade

    Full Text Available ABSTRACT: Euxylophora paraensis Huber - Rutaceae, popularly known as yellow heart, is a species with a low-density population that has suffered intense exploitation in recent decades. This has led to its inclusion in the IBAMA list of endangered species. This study aimed to evaluate the existence of growth rings and their annuity in five trees of Euxylophora paraensis through the x-ray microdensitometry technique, as well as to compare them using the classical method. Application feasibility of this technique can be deepened through future, broader dendroecological studies, which may better elucidate the understanding of the species population dynamics and promote more sustainable uses. Wood disks were collected from the base of five 29-year-old trees from a planting site in the Curua-Una Experimental Station, located in Prainha, Pará State, Brazil. Samples were polished and sanded for the x-ray microdensitometric analysis. Values of minimum, average, and maximum density (0.581, 0.84, and 1.077gcm-3, respectively were obtained. There was a significant difference in density between the studied trees. Growth rings boundaries, through densitometric pith-bark profile are demarcated by fibrous zones, with density variation between the early wood and late wood. This study concludes that the growth rings of the studied species are distinct and annual, validating the technique used.

  20. The Complete Chloroplast Genome Sequence of Tree of Heaven (Ailanthus altissima (Mill. (Sapindales: Simaroubaceae, an Important Pantropical Tree

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    Josphat K. Saina

    2018-03-01

    Full Text Available Ailanthus altissima (Mill. Swingle (Simaroubaceae is a deciduous tree widely distributed throughout temperate regions in China, hence suitable for genetic diversity and evolutionary studies. Previous studies in A. altissima have mainly focused on its biological activities, genetic diversity and genetic structure. However, until now there is no published report regarding genome of this plant species or Simaroubaceae family. Therefore, in this paper, we first characterized A. altissima complete chloroplast genome sequence. The tree of heaven chloroplast genome was found to be a circular molecule 160,815 base pairs (bp in size and possess a quadripartite structure. The A. altissima chloroplast genome contains 113 unique genes of which 79 and 30 are protein coding and transfer RNA (tRNA genes respectively and also 4 ribosomal RNA genes (rRNA with overall GC content of 37.6%. Microsatellite marker detection identified A/T mononucleotides as majority SSRs in all the seven analyzed genomes. Repeat analyses of seven Sapindales revealed a total of 49 repeats in A. altissima, Rhus chinensis, Dodonaea viscosa, Leitneria floridana, while Azadirachta indica, Boswellia sacra, and Citrus aurantiifolia had a total of 48 repeats. The phylogenetic analysis using protein coding genes revealed that A. altissima is a sister to Leitneria floridana and also suggested that Simaroubaceae is a sister to Rutaceae family. The genome information reported here could be further applied for evolution and invasion, population genetics, and molecular studies in this plant species and family.

  1. RESPOSTA FUNCIONAL DA JOANINHA CRYPTOLAEMUS PREDANDO COCHONILHA BRANCA EM DIFERENTES TEMPERATURAS E SUBSTRATOS VEGETAIS

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    SERGIO ANTONIO DE BORTOLI

    2014-01-01

    Full Text Available This work was carried out to evaluate the functional response of Cryptolaemus montrouzieri Mulsant, 1850 (Coleoptera: Coccinellidae fed with Planococcus citri Risso, 1813 (Hemiptera: Pseudococcidae reared on a pumpkin hybrid (Cucurbita maxima x Cucurbita moscata (Cucurbitaceae, seedlings of Rangpur lime (Citrus limonia Rutaceae and potato (Solanum tuberosum (Solanaceae at two temperatures. The predation rate of C. montrouzieri was measured using Petri dishes of 15 cm diameter with 1, 2, 4, 8, 16 and 24 adults of P. Citri. One third instar larva, one fourfh instar and one newly emerged adult (without differentiation of sex of C. montrouzieri were added to each plate. The study was conducted in climatic chambers at temperatures of 25 and 30 º C and photophase of 12 hours. The predation rate was evaluated after 24 hours of prey exposition to the predator, by counting the number of preys trapped in the different treatments and control. The statistical design was completely randomized with four treatments x 6 subplots with 7 repetitions, the two temperatures. The values obtained were subjected to analysis of variance, to relate the number of scales preyed by larvae and adults of C. montrouzieri set up in different substrates. The amount of prey consumed by larvae and adults of the predator increased with increasing the prey density until it reaches a plateau, characterizing functional response type II. In general, the number of scales preyed by larvae and adults of C. montrouzieri was higher on potato and under temperature of 30 °C.

  2. Advances in Studies on Natural Preservativesfor Fruits and Vegetables

    Science.gov (United States)

    Gao, Haisheng; Shi, Pengbao; Zhao, Yuhua

    The author introduced g eneral research and application situations of natural preservatives for fruits and vegetables all over the world these years, and summarized application of vegetation of Murraya in Rutaceae, Cinnamomum in Lauraceae, Artemisia in Compositae and other families and genera on fruits and vegetables preservation and fresh-keeping. Decoction or extraction of Chinese traditional medicine, such as Alpinia Officinarum, Amarphalus Konjac K., stemona etc, could be used in fresh-keeping for orange, apple, strawberry, edible fungi and so on. Garlic could be used in fresh-keeping for orange. Phytic acid and fresh-keeping agents compounded with Phytic acid could extend storage periods of easily rotting fruits and vegetables, such as strawberry, banana, cantaloup, edible fungi and so on, and better keep original fresh condition. Extraction of Snow Fresh, Semper Fresh, Arthropod shell extraction, and halite also had better effect on preservation and fresh-keeping for fruits and vegetables. Main problems exsited in the application of natural preservatives for fruits and vegetables were showed in this article and the applying prospect were discussed too.

  3. Phytochemical Evaluation, Antimicrobial Activity, and Determination of Bioactive Components from Leaves of Aegle marmelos

    Science.gov (United States)

    Mujeeb, Farina; Bajpai, Preeti; Pathak, Neelam

    2014-01-01

    The therapeutic value of Aegle marmelos Correa (Rutaceae), commonly known as ‘‘Bael,” has been recognized as a component of traditional medication for the treatment of various human ailments. The plant, though, being highly explored, still lacks sufficient evidences for the best variety possessing the highest degree of medicinal values. The present study is focused on phytochemical screening of aqueous and methanolic leaf extracts of 18 varieties/accessions of A. marmelos. The crude extracts of A. marmelos revealed the presence of several biologically active phytochemicals with the highest quantity of alkaloids, flavonoids, and phenols in Pant Aparna variety. The antibacterial efficacy was investigated against pathogenic bacterial strains and the highest inhibitory activity of aqueous extract was obtained against S. epidermidis, whereas methanolic extract was found to be most potent against S. aureus at 40 mg/mL concentration. However, in aqueous : ethanol, the best results were observed against E. aerogenes followed by K. pneumonia and S. epidermidis. The MIC of aqueous and methanol extract of Aegle marmelos ranged from 10 mg/mL to 40 mg/mL whereas in aqueous : ethanol it ranged between 40 mg/mL and 160 mg/mL. The GC-MS analysis revealed the presence of many bioactive compounds such as flavonoids, alcohols, aldehydes, aromatic compounds, fatty acid methyl esters, terpenoids, phenolics, and steroids that can be postulated for antibacterial activity. PMID:24900969

  4. In vitro antibacterial effects of Zanthoxylum tingoassuiba root bark extracts and two of its alkaloids against multiresistant Staphylococcus aureus

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    Rafael S. Costa

    Full Text Available ABSTRACT The emergence of multiresistant strains of bacteria reinforces the need to search for new compounds able to combat resistant organisms. Medicinal plants are a great resource of bioactive substances, providing the possibility of obtaining molecules with potential antimicrobial activity. The aim of the present study is the evaluation of the antibacterial activity of extracts and alkaloids isolated from the root bark of Zanthoxylum tingoassuiba A. St.-Hil., Rutaceae, against four resistant clinical isolates and Staphylococcus aureus ATCC 25923. The dichloromethane and methanol extracts were fractionated by chromatography on silica gel, leading to the isolation of dihydrocheleryhtrine and N-methylcanadine, identified by Nuclear Magnetic Resonance spectroscopy. The antibacterial activity of the extracts and isolated compounds was evaluated by the disc diffusion method and the minimum inhibitory concentration was determined. The dichloromethane extract was the most active against all the tested strains and the two pure alkaloids were more active than the extracts. The anti-MRSA activity of the two benzophenanthridine alkaloids is demonstrated for the first time in this study. These compounds appear as potential leads for the development of new anti-MRSA compounds and could be responsible for the antibacterial activity, justifying the ethnobotanical use of Z. tingoassuiba and other species for the treatment of various infectious diseases.

  5. Modification of radiation induced intestinal lesions by Aegle marmelos fruit extract, an Indian medicinal plant

    International Nuclear Information System (INIS)

    Agrawal, Annapurna; Jahan, Swafiya; Goyal, R.K.

    2012-01-01

    Recently, some plant extracts have been screened out against radiation and found to be quite promising. Aegle marmelos (Bael), belonging to family Rutaceae, fruits are very good source of proteins which form 5.12% of the edible portion. It is claimed to be useful in treating pain, fever, inflammation, respiratory disorders, cardiac disorders, dysentery and diarrhea. The ripe fruit is used for the treatment of digestive and stomachic complications. Aegle marmelos is a useful medicine for herbalist and it holds a reputed position in Ayurvedic system of medicine. Protection of intestinal constituents by Aegle marmelos extract (AME) was studied after exposure to 6 Gy gamma radiations in mice. For this purpose, Swiss albino mice were divided in various groups. Group I was administered with double distilled water (DDW), volume equal to AME (100 mg/kg body wt./animal), by oral gavage to serve as normal. Group II was administered orally AME extract once daily at a dose of 100 mg/kg b.wt./animal for 5 consecutive days. Group III was exposed to 6 Gy gamma radiations to serve as irradiated control. Group IV was treated with AME, orally for 5 consecutive days (as in Group-II), and were exposed to gamma radiation half an hour after the last administration of AME on day 5. Animals from all these group autopsied on 12 hrs, days 1, 3, 7, 15 and 30 post-treatment intervals

  6. Secondary metabolites of ponderosa lemon (Citrus pyriformis) and their antioxidant, anti-inflammatory, and cytotoxic activities.

    Science.gov (United States)

    Hamdan, Dalia; El-Readi, Mahmoud Zaki; Tahrani, Ahmad; Herrmann, Florian; Kaufmann, Dorothea; Farrag, Nawal; El-Shazly, Assem; Wink, Michael

    2011-01-01

    Column chromatography of the dichloromethane fraction from an aqueous methanolic extract of fruit peel of Citrus pyriformis Hassk. (Rutaceae) resulted in the isolation of seven compounds including one coumarin (citropten), two limonoids (limonin and deacetylnomilin), and four sterols (stigmasterol, ergosterol, sitosteryl-3-beta-D-glucoside, and sitosteryl-6'-O-acyl-3-beta-D-glucoside). From the ethyl acetate fraction naringin, hesperidin, and neohesperidin were isolated. The dichloromethane extract of the defatted seeds contained three additional compounds, nomilin, ichangin, and cholesterol. The isolated compounds were identified by MS (EI, CI, and ESI), 1H, 13C, and 2D-NMR spectral data. The limonoids were determined qualitatively by LC-ESI/MS resulting in the identification of 11 limonoid aglycones. The total methanolic extract of the peel and the petroleum ether, dichloromethane, and ethyl acetate fractions were screened for their antioxidant and anti-inflammatory activities. The ethyl acetate fraction exhibited a significant scavenging activity for DPPH free radicals (IC50 = 132.3 microg/mL). The petroleum ether fraction inhibited 5-lipoxygenase with IC50 = 30.6 microg/mL indicating potential anti-inflammatory properties. Limonin has a potent cytotoxic effect against COS7 cells [IC50 = (35.0 +/- 6.1) microM] compared with acteoside as a positive control [IC50 = (144.5 +/- 10.96) microM].

  7. Seasonal change in main alkaloids of jaborandi (Pilocarpus microphyllus Stapf ex Wardleworth, an economically important species from the Brazilian flora.

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    David Fernandes Lima

    Full Text Available Pilocarpus microphyllus Stapf ex Wardleworth (jaborandi, Rutaceae is one of the most important Brazilian medicinal species owing to its content of pilocarpine (PIL, an alkaloid used for treating glaucoma and xerostomia. This species contains another alkaloid, epiisopiloturine (EPI, which has demonstrated effectiveness against schistosomiasis. The aim of this work was to assess seasonal changes of PIL and EPI in three populations of cultivated P. microphyllus from northeastern Brazil over one year, including the dry and rainy seasons. Alkaloid profiles were correlated to phenotypic and genetic patterns in the morphological and molecular characterizations. PIL was the primary alkaloid and its levels differed among populations in all months except September. The S01 population (green line showed an especially high PIL content compared to populations S02 and S03 (traditional line, which had similar alkaloid contents. PIL content gradually decreased in the three populations in the rainy season.EPI content was significantly different between the green line (S01 and the traditional line (S02 and S03.S01 had a significantly lower EPI content in all months, demonstrating that it was not the best source for EPI extraction. Inter simple sequence repeat (ISSR markers and morphological analyses clearly separated S01 from S02 and S03, in agreement with the alkaloid results. This study shows the first correlation between the chemical, morphological, and molecular markers of P. microphyllus and highlights the potential benefits of a multidisciplinary research approach aimed at supporting both industry and conservation of natural resources.

  8. Antioxidant capacity of hesperidin from citrus peel using electron spin resonance and cytotoxic activity against human carcinoma cell lines.

    Science.gov (United States)

    Al-Ashaal, Hanan A; El-Sheltawy, Shakinaz T

    2011-03-01

    Hesperidin is a flavonoid that has various pharmacological activities including anti-inflammatory, antimicrobial and antiviral activities. The aim of the study is the isolation of hesperidin from the peel of Citrus sinensis L. (Rutaceae), and the evaluation of its antioxidant capacity and cytotoxicity against different human carcinoma cell lines. In the present work, hesperidin is identified and confirmed using chromatographic and spectral analysis. To correlate between hesperidin concentration and antioxidant capacity of peel extracts, extraction was carried out using 1% HCl-MeOH, MeOH, alkaline solution, the concentration of hesperidin determined qualitatively and quantitatively using high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC) analysis, in vitro antioxidant capacity of hesperidin and the extracts against free radical diphenylpicrylhydrazyl (DPPH•) performed using an electron spin resonance spectrophotometer (ESR). Cytotoxic assay against larynx, cervix, breast and liver carcinoma cell lines was performed. Hesperidin was found to be moderately active as an antioxidant agent; its capacity reached 36%. In addition, the results revealed that hesperidin exhibited pronounced anticancer activity against the selected cell lines. IC₅₀ were 1.67, 3.33, 4.17, 4.58 µg/mL, respectively. Orange peels are considered to be a cheap source for hesperidin which may be used in the pharmaceutical industry as a natural chemopreventive agent. Hesperidin and orange peel extract could possess antioxidant properties with a wide range of therapeutic applications.

  9. The protective role of Aegle marmelos on aspirin-induced gastro-duodenal ulceration in albino rat model: a possible involvement of antioxidants.

    Science.gov (United States)

    Das, Shyamal K; Roy, Chandan

    2012-01-01

    Gastro duodenal ulcer is a common disorder of the gastrointestinal tract. Several Indian medicinal plants have been traditionally and extensively used to prevent different diseases. In the present research studies, Bael fruit (Aegle marmelos (AM), family: Rutaceae) which are also called as Bilva in ancient Sanskrit was used as a herbal drug and its antioxidative role in aspirin- induced gastroduodenal ulceration in albino rat was evaluated using essential biochemical parameters. Mucosal thickness (MT), ulcer index (UI), different biochemical parameters, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), and lipid peroxidation (LPO) were measured in all the groups, to study the possible involvement of antioxidants with gastroduodenal protection. A significant decrease in MT, SOD and CAT activities and GSH level and a significant increase in UI, AST, ALT, and ALP activities and LPO level were observed in aspirin treated stomach and duodenum of albino rats. Pretreatment with AM fruit pulp extract for 14 consecutive days showed the reverse effects of aspirin suggesting gastro-duodenal protective and anti- ulcerogenic properties of AM through its antioxidant mechanism.

  10. Diversity of Medicinal Plants in the Flora of Saudi Arabia 3: An inventory of 15 Plant Families and their Conservation Management

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    Mohammed Yusuf

    2014-09-01

    Full Text Available An inventory of medicinal species diversity in the flora of the Kingdom of Saudi Arabia has been made for 15 angiosperm families, viz., Boraginaceae, Convolvulaceae, Cucurbitaceae, Fabaceae, Molluginaceae, Papavaraceae, Portulacaceae, Ranunculaceae, Rhamnaceae, Rutaceae, Tamaricaceae, Tiliaceae, Urticaceae, Verbenaceae and Vitaceae, and 61 species of medicinal plants are recognized. These families are represented in the flora by a total of 393 species of which 15.52% are medicinal. Among the families, the Fabaceae is found to be represented by 23 medicinal species which is highest and 37.70% of the total species. Of these 61 medicinal species, 72.13% exhibits herbaceous life form while remaining 13.11% and 14.75% exhibit shrubs and trees respectively. An enumeration of these medicinal species is presented, each with current nomenclature, Arabic name, English name, medicinal uses, pharmacological properties and status of occurrence in the flora. The communication is aimed at emphasizing the planning and implementation of national conservation strategies for sustainable management of the medicinal plants of the Kingdom of Saudi Arabia. DOI: http://dx.doi.org/10.3126/ije.v3i3.11091 International Journal of Environment Vol.3(3 2014: 312-320

  11. Genetic relationships among wild and cultivated accessions of curry leaf plant (Murraya koenigii (L.) Spreng.), as revealed by DNA fingerprinting methods.

    Science.gov (United States)

    Verma, Sushma; Rana, T S

    2013-02-01

    Murraya koenigii (L.) Spreng. (Rutaceae), is an aromatic plant and much valued for its flavor, nutritive and medicinal properties. In this study, three DNA fingerprinting methods viz., random amplification of polymorphic DNA (RAPD), directed amplification of minisatellite DNA (DAMD), and inter-simple sequence repeat (ISSR), were used to unravel the genetic variability and relationships across 92 wild and cultivated M. koenigii accessions. A total of 310, 102, and 184, DNA fragments were amplified using 20 RAPD, 5 DAMD, and 13 ISSR primers, revealing 95.80, 96.07, and 96.73% polymorphism, respectively, across all accessions. The average polymorphic information content value obtained with RAPD, DAMD, and ISSR markers was 0.244, 0.250, and 0.281, respectively. The UPGMA tree, based on Jaccard's similarity coefficient generated from the cumulative (RAPD, DAMD, and ISSR) band data showed two distinct clusters, clearly separating wild and cultivated accessions in the dendrogram. Percentage polymorphism, gene diversity (H), and Shannon information index (I) estimates were higher in cultivated accessions compared to wild accessions. The overall high level of polymorphism and varied range of genetic distances revealed a wide genetic base in M. koenigii accessions. The study suggests that RAPD, DAMD, and ISSR markers are highly useful to unravel the genetic variability in wild and cultivated accessions of M. koenigii.

  12. Seasonal change in main alkaloids of jaborandi (Pilocarpus microphyllus Stapf ex Wardleworth), an economically important species from the Brazilian flora

    Science.gov (United States)

    Véras, Leiz Maria Costa; Azevedo, Iábita Fabiana Sousa; Biase, Adriele Giaretta; Costa, Joana; Oliveira, Maria Beatriz P. P.; Mafra, Isabel

    2017-01-01

    Pilocarpus microphyllus Stapf ex Wardleworth (jaborandi, Rutaceae) is one of the most important Brazilian medicinal species owing to its content of pilocarpine (PIL), an alkaloid used for treating glaucoma and xerostomia. This species contains another alkaloid, epiisopiloturine (EPI), which has demonstrated effectiveness against schistosomiasis. The aim of this work was to assess seasonal changes of PIL and EPI in three populations of cultivated P. microphyllus from northeastern Brazil over one year, including the dry and rainy seasons. Alkaloid profiles were correlated to phenotypic and genetic patterns in the morphological and molecular characterizations. PIL was the primary alkaloid and its levels differed among populations in all months except September. The S01 population (green line) showed an especially high PIL content compared to populations S02 and S03 (traditional line), which had similar alkaloid contents. PIL content gradually decreased in the three populations in the rainy season.EPI content was significantly different between the green line (S01) and the traditional line (S02 and S03).S01 had a significantly lower EPI content in all months, demonstrating that it was not the best source for EPI extraction. Inter simple sequence repeat (ISSR) markers and morphological analyses clearly separated S01 from S02 and S03, in agreement with the alkaloid results. This study shows the first correlation between the chemical, morphological, and molecular markers of P. microphyllus and highlights the potential benefits of a multidisciplinary research approach aimed at supporting both industry and conservation of natural resources. PMID:28151972

  13. Influence of Solvent Polarity and DNA-Binding on Spectral Properties of Quaternary Benzo[c]phenanthridine Alkaloids.

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    Michal Rájecký

    Full Text Available Quaternary benzo[c]phenanthridine alkaloids are secondary metabolites of the plant families Papaveraceae, Rutaceae, and Ranunculaceae with anti-inflammatory, antifungal, antimicrobial and anticancer activities. Their spectral changes induced by the environment could be used to understand their interaction with biomolecules as well as for analytical purposes. Spectral shifts, quantum yield and changes in lifetime are presented for the free form of alkaloids in solvents of different polarity and for alkaloids bound to DNA. Quantum yields range from 0.098 to 0.345 for the alkanolamine form and are below 0.033 for the iminium form. Rise of fluorescence lifetimes (from 2-5 ns to 3-10 ns and fluorescence intensity are observed after binding of the iminium form to the DNA for most studied alkaloids. The alkanolamine form does not bind to DNA. Acid-base equilibrium constant of macarpine is determined to be 8.2-8.3. Macarpine is found to have the highest increase of fluorescence upon DNA binding, even under unfavourable pH conditions. This is probably a result of its unique methoxy substitution at C12 a characteristic not shared with other studied alkaloids. Association constant for macarpine-DNA interaction is 700000 M(-1.

  14. PLANTAS ÚTILES EN LOS SISTEMAS AGROFORESTALES TRADICIONALES DEL LITORAL ECUATORIANO

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    Luz García Cruzatty

    2008-12-01

    Full Text Available El estudio se realizó en la Cuenca Media del Río Guayas, Ecuador, en el área correspondiente a los cantones Mocache y Palenque. El inventario de las especies se obtuvo mediante recorridos participativos. Las especies se clasificaron de acuerdo al Sistema Filogenético de Cronquist. Los datos sobre el uso de las especies se obtuvieron mediante charlas y entrevistas no formales. Se determinó que los agricultores de la Cuenca Media del Río Guayas conocen y utilizan 262 especies vegetales que pertenecen a 67 familias y 164 géneros. Las familias vegetales más comunes fueron: Anacardiaceae, Papilonaceae, Solanaceae, Rutaceae, Musaceae y Lamiaceae, con más de diez especies cada una. Se documentaron 21 tipos de usos, de los cuales los más importantes son: alimenticio, medicinal y construcción, lo que demuestra la preocupación de las familias por satisfacer sus necesidades básicas. La mayoría de las especies utilizadas son árboles, seguido por hierbas y arbustos. Entre las especies de mayor importancia por los múltiples usos y productos que se obtienen están: Persea americana Mill. (aguacate, Artocarpus altilis Fosberg (fruta de pan, Musa sp. (plátano, Manguifera sp. (mango, Phythelephas aequatoriales Spruce (cade, Inga edulis Matius (guaba de bejuco, entre otros.

  15. PATENT ANALYSIS: A LOOK AT THE INNOVATIVE NATURE OF PLANT-BASED COSMETICS

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    Francine C. S. César

    Full Text Available The use of plants have become a important trend in cosmetic market, with a crescent number of patent applications. Despite various herbal-based ingredients have been described in scientific literature, most of them are restricted to patent databases. In this work we analyze the innovative use of plants and its derivatives in cosmetic technologies, based on patent analysis. The investigation was conducted using Derwent World Patents Index (Thomson Reuters Scientific, USA from 1995 to 2015. The search was carried out to evaluate the number of patent deposits, the patent depositors profiles, the countries with most patent applications, the International Patent Classification (IPC and the main plant families using in cosmetics. Patent analysis revealed an increase number of applications involving cosmetic containing plants components, with a higher number of deposits related to anti-ageing and whitening skin treatment. Fabaceae, Asteraceae, Rosaceae, Lamiaceae, Poaceae, Rutaceae, Lilliacae and Apiaceae were the key plant families used in cosmetic formulations for skin care treatment. Comparison between scientific and technological data pointed out divergences between patents deposits and aid-based scientific reports. The use of patent analysis in combination with scientific data opens up wider aspects of knowledge and enables a better rationalization of innovative works.

  16. Evaluation of central nervous system effects of Citrus limon essential oil in mice

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    Lidianne Mayra Lopes Campêlo

    2011-05-01

    Full Text Available The central nervous system (CNS depressant and anticonvulsant activities of Citrus limon (L. Osbeck, Rutaceae, essential oil (EO were investigated in animal models. The EO (50, 100 and 150 mg/kg injected by oral route (p.o. in mice caused a significant decrease in the motor activity of animals when compared with the control group, up to thirty days after the administration and the dose of 150 mg/kg significantly reduced the remaining time of the animals on the Rota-rod apparatus. Additionally, C. limon essential oil was also capable to promote an increase of latency for development of convulsions induced by pentylenetetrazole (PTZ. The administration of FLU (10 mg/kg, i.p., GABA A-benzodiazepine (GABA-BZD receptor antagonist, antagonized the effect of C. limon essential oil at higher dose. This C. limon essential oil was also capable to promote an increase of latency for development of convulsions induced by picrotoxin (PIC at higher dose. In the same way, the anticonvulsant effect of the EO was affected by pretreatment with flumazenil, a selective antagonist of benzodiazepine site of GABA A receptor. These results suggest a possible CNS depressant and anticonvulsant activities in mice that needs further investigation.

  17. Acetylcholinesterase inhibition by somes promising Brazilian medicinal plants.

    Science.gov (United States)

    Feitosa, C M; Freitas, R M; Luz, N N N; Bezerra, M Z B; Trevisan, M T S

    2011-08-01

    A microplate assay and a thin-layer chromatography (TLC) "in situ" assay based on the Ellman assay was used to screen for acetylcholinesterase inhibitors from ethyl acetate and methanol extracts of Brazilian medicinal plants of families that, according to the literature, have traditional uses that might be connected with acetylcholinesterase inhibition. Eighteen species belonging to Convolvulaceae, Crassulaceae, Euphorbiaceae, Leguminosae, Malvaceae, Moraceae, Nyctaginaceae and Rutaceae families were tested. The most active plants were Ipomoea asarifolia (IC50 = 0.12 mg/mL), Jatropha curcas (IC50 = 0.25 mg/mL), Jatropha gossypiifolia (IC50 = 0.05 mg/mL), Kalanchoe brasiliensis (IC50 = 0.16 mg/mL) and Senna alata (IC50 = 0.08 mg/mL). The most promising extracts were the Jatropha gossypiifolia and Senna alata species assuming there were compounds with a similar activity to galanthamine, which should contain about 1% of an active compound, or if present at lower levels even more active compounds than galanthamine (IC50 = 0.37 x 10-3 mg/mL) should be present.

  18. Mechanism involved in the anti-inflammatory effect of Spiranthera odoratissima (Manacá

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    Daniela B. M. Barbosa

    2012-02-01

    Full Text Available Acetic acid-induced writhing, hot-plate, carrageenan-induced pleurisy, formalin-induced pain, croton oil-induced ear edema, vascular permeability tests and phospholipase A2 activity assay were used to study the analgesic and/or anti-inflammatory activity of the hydromethanolic fraction of ethanolic extract from Spiranthera odoratissima A. St.-Hil., Rutaceae, leaves (HMF and its subfraction (sub-Fr10-28. HMF and sub-Fr10-28 reduced the leukocyte migration on the carrageenan-induced pleurisy test; sub-Fr10-28 reduced the pain reaction time in the second phase of formalin-induced pain, as well as the ear edema and vascular permeability. Both HMF and sub-Fr10-28 inhibited the phospholipase A2 activity. These results suggest that the analgesic effect of this plant could be, in part, due to an anti-inflammatory action produced by the inhibition of phospholipase A2 activity.

  19. The Methanolic Extract from Murraya koenigii L. Inhibits Glutamate-Induced Pain and Involves ATP-Sensitive K+ Channel as Antinociceptive Mechanism

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    Nushrat Sharmin Ani

    2016-01-01

    Full Text Available Murraya koenigii L. is a perennial shrub, belonging to the family Rutaceae. Traditionally, the leaves of this plant are extensively used in treatment of a wide range of diseases and disorders including pain and inflammation. Although researchers have revealed the antinociceptive effects of this plant’s leaves during past few years, the mechanisms underlying these effects are still unknown. Therefore, the present study evaluated some antinociceptive mechanisms of the methanolic extract of M. koenigii (MEMK leaves along with its antinociceptive potential using several animal models. The antinociceptive effects of MEMK were evaluated using formalin-induced licking and acetic acid-induced writhing tests at the doses of 50, 100, and 200 mg/kg. In addition, we also justified the possible participations of glutamatergic system and ATP-sensitive potassium channels in the observed activities. Our results demonstrated that MEMK significantly (p<0.01 inhibited the pain thresholds induced by formalin and acetic acid in a dose-dependent manner. MEMK also significantly (p<0.01 suppressed glutamate-induced pain. Moreover, pretreatment with glibenclamide (an ATP-sensitive potassium channel blocker at 10 mg/kg significantly (p<0.05 reversed the MEMK-mediated antinociception. These revealed that MEMK might have the potential to interact with glutamatergic system and the ATP-sensitive potassium channels to exhibit its antinociceptive activities. Therefore, our results strongly support the antinociceptive effects of M. koenigii leaves and provide scientific basis of their analgesic uses in the traditional medicine.

  20. Biological Activity of Carbazole Alkaloids and Essential Oil of Murraya koenigii Against Antibiotic Resistant Microbes and Cancer Cell Lines

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    Thilahgavani Nagappan

    2011-11-01

    Full Text Available A total of three carbazole alkaloids and essential oil from the leaves of Murraya koenigii (Rutaceae were obtained and examined for their effects on the growth of five antibiotic resistant pathogenic bacteria and three tumor cell lines (MCF-7, P 388 and Hela. The structures of these carbazoles were elucidated based on spectroscopy data and compared with literature data, hence, were identified as mahanine (1, mahanimbicine (2 and mahanimbine (3. The chemical constituents of the essential oil were identified using Gas Chromatography-Mass Spectroscopy (GCMS. These compounds exhibited potent inhibition against antibiotic resistant bacteria such as Staphylococcus aureus (210P JTU, Psedomonas aeruginosa (ATCC 25619, Klebsiella pneumonia (SR1-TU, Escherchia coli (NI23 JTU and Streptococcus pneumoniae (SR16677-PRSP with significant minimum inhibition concentration (MIC values (25.0–175.0 mg/mL and minimum bacteriacidal concentrations (MBC (100.0–500.0 mg/mL. The isolated compounds showed significant antitumor activity against MCF-7, Hela and P388 cell lines. Mahanimbine (3 and essential oil in particular showed potent antibacteria and cytotoxic effect with dose dependent trends (≤5.0 μg/mL. The findings from this investigation are the first report of carbazole alkaloids’ potential against antibiotic resistant clinical bacteria, MCF-7 and P388 cell lines.

  1. A Nuclear Ribosomal DNA Phylogeny of Acer Inferred with Maximum Likelihood, Splits Graphs, and Motif Analysis of 606 Sequences

    Science.gov (United States)

    Grimm, Guido W.; Renner, Susanne S.; Stamatakis, Alexandros; Hemleben, Vera

    2007-01-01

    The multi-copy internal transcribed spacer (ITS) region of nuclear ribosomal DNA is widely used to infer phylogenetic relationships among closely related taxa. Here we use maximum likelihood (ML) and splits graph analyses to extract phylogenetic information from ~ 600 mostly cloned ITS sequences, representing 81 species and subspecies of Acer, and both species of its sister Dipteronia. Additional analyses compared sequence motifs in Acer and several hundred Anacardiaceae, Burseraceae, Meliaceae, Rutaceae, and Sapindaceae ITS sequences in GenBank. We also assessed the effects of using smaller data sets of consensus sequences with ambiguity coding (accounting for within-species variation) instead of the full (partly redundant) original sequences. Neighbor-nets and bipartition networks were used to visualize conflict among character state patterns. Species clusters observed in the trees and networks largely agree with morphology-based classifications; of de Jong’s (1994) 16 sections, nine are supported in neighbor-net and bipartition networks, and ten by sequence motifs and the ML tree; of his 19 series, 14 are supported in networks, motifs, and the ML tree. Most nodes had higher bootstrap support with matrices of 105 or 40 consensus sequences than with the original matrix. Within-taxon ITS divergence did not differ between diploid and polyploid Acer, and there was little evidence of differentiated parental ITS haplotypes, suggesting that concerted evolution in Acer acts rapidly. PMID:19455198

  2. Phytochemical evaluation, antimicrobial activity, and determination of bioactive components from leaves of Aegle marmelos.

    Science.gov (United States)

    Mujeeb, Farina; Bajpai, Preeti; Pathak, Neelam

    2014-01-01

    The therapeutic value of Aegle marmelos Correa (Rutaceae), commonly known as ''Bael," has been recognized as a component of traditional medication for the treatment of various human ailments. The plant, though, being highly explored, still lacks sufficient evidences for the best variety possessing the highest degree of medicinal values. The present study is focused on phytochemical screening of aqueous and methanolic leaf extracts of 18 varieties/accessions of A. marmelos. The crude extracts of A. marmelos revealed the presence of several biologically active phytochemicals with the highest quantity of alkaloids, flavonoids, and phenols in Pant Aparna variety. The antibacterial efficacy was investigated against pathogenic bacterial strains and the highest inhibitory activity of aqueous extract was obtained against S. epidermidis, whereas methanolic extract was found to be most potent against S. aureus at 40 mg/mL concentration. However, in aqueous : ethanol, the best results were observed against E. aerogenes followed by K. pneumonia and S. epidermidis. The MIC of aqueous and methanol extract of Aegle marmelos ranged from 10 mg/mL to 40 mg/mL whereas in aqueous : ethanol it ranged between 40 mg/mL and 160 mg/mL. The GC-MS analysis revealed the presence of many bioactive compounds such as flavonoids, alcohols, aldehydes, aromatic compounds, fatty acid methyl esters, terpenoids, phenolics, and steroids that can be postulated for antibacterial activity.

  3. Antibacterial and antifungal activity of Flindersine isolated from the traditional medicinal plant, Toddalia asiatica (L.) Lam.

    Science.gov (United States)

    Duraipandiyan, V; Ignacimuthu, S

    2009-06-25

    The leaves and root of Toddalia asiatica (L.) Lam. (Rutaceae) are widely used as a folk medicine in India. Hexane, chloroform, ethyl acetate, methanol and water extracts of Toddalia asiatica leaves and isolated compound Flindersine were tested against bacteria and fungi. Antibacterial and antifungal activities were tested against bacteria and fungi using disc-diffusion method and minimum inhibitory concentrations (MICs). The compound was confirmed using X-ray crystallography technique. Antibacterial and antifungal activities were observed in ethyl acetate extract. One active principle Flindersine (2,6-dihydro-2,2-dimethyl-5H-pyrano [3,2-c] quinoline-5-one-9cl) was isolated from the ethyl acetate extract. The MIC values of the compound against bacteria Bacillus subtilis (31.25 microg/ml), Staphylococcus aureus (62.5 microg/ml), Staphylococcus epidermidis (62.5 microg/ml), Enterococcus faecalis (31.25 microg/ml), Pseudomonas aeruginosa (250 microg/ml), Acinetobacter baumannii (125 microg/ml) and fungi Trichophyton rubrum 57 (62.5 microg/ml), Trichophyton mentagrophytes (62.5 microg/ml), Trichophyton simii (62.5 microg/ml), Epidermophyton floccosum (62.5 microg/ml), Magnaporthe grisea (250 microg/ml) and Candida albicans (250 microg/ml) were determined. Ethyl acetate extract showed promising antibacterial and antifungal activity and isolated compound Flindersine showed moderate activity against bacteria and fungi.

  4. In vitro anti-Helicobacter pylori action of 30 Chinese herbal medicines used to treat ulcer diseases.

    Science.gov (United States)

    Li, Yang; Xu, Chen; Zhang, Qiang; Liu, Jun Yan; Tan, Ren Xiang

    2005-04-26

    Infection by Helicobacter pylori has been ascertained to be an important etiologic impetus leading usually to chronic active gastritis and gastric ulcer with growing incidences worldwide. Utilizing as the test pathogen a standard and five clinic strains of Helicobacter pylori, the antibacterial action was assessed in vitro with ethanol extracts of 30 Chinese herbal medicines which have been frequently prescribed since ancient times for treating gastritis-like disorders. Among the 30 tested materials, the ethanol extracts of Abrus cantoniensis (Fabaceae), Saussurea lappa (Asteraceae) and Eugenia caryophyllata (Myrtaceae) were strongly inhibitory to all test strains (MICs: approximately 40 microg/ml), and Hippophae rhamnoides (Elaeagnaceae), Fritillaria thunbergii (Liliaceae), Magnolia officinalis and Schisandra chinensis (Magnoliaceae), Corydalis yanhusuo (Papaveraceae), Citrus reticulata (Rutaceae), Bupleurum chinense and Ligusticum chuanxiong (Apiaceae) substantially active with MICs close to 60.0 microg/ml. As to antibacterial actions of the aqueous extracts of the same drugs, those derived from Cassia obtusifolia (Fabaceae), Fritillaria thunbergii and Eugenia caryophyllata were remarkably inhibitory against all the six Helicobacter pylori strains (MICs: approximately 60 microg/ml). The work compared almost quantitatively the magnitude of the anti-Helicobacter pylori actions of the 30 most prescribed gastritis-treating Chinese herbal drugs, and located as well some source plants where potent anti-Helicobacter pylori phytochemicals could be characterized.

  5. A Nuclear Ribosomal DNA Phylogeny of Acer Inferred with Maximum Likelihood, Splits Graphs, and Motif Analysis of 606 Sequences

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    Guido W. Grimm

    2006-01-01

    Full Text Available The multi-copy internal transcribed spacer (ITS region of nuclear ribosomal DNA is widely used to infer phylogenetic relationships among closely related taxa. Here we use maximum likelihood (ML and splits graph analyses to extract phylogenetic information from ~ 600 mostly cloned ITS sequences, representing 81 species and subspecies of Acer, and both species of its sister Dipteronia. Additional analyses compared sequence motifs in Acer and several hundred Anacardiaceae, Burseraceae, Meliaceae, Rutaceae, and Sapindaceae ITS sequences in GenBank. We also assessed the effects of using smaller data sets of consensus sequences with ambiguity coding (accounting for within-species variation instead of the full (partly redundant original sequences. Neighbor-nets and bipartition networks were used to visualize conflict among character state patterns. Species clusters observed in the trees and networks largely agree with morphology-based classifications; of de Jong’s (1994 16 sections, nine are supported in neighbor-net and bipartition networks, and ten by sequence motifs and the ML tree; of his 19 series, 14 are supported in networks, motifs, and the ML tree. Most nodes had higher bootstrap support with matrices of 105 or 40 consensus sequences than with the original matrix. Within-taxon ITS divergence did not differ between diploid and polyploid Acer, and there was little evidence of differentiated parental ITS haplotypes, suggesting that concerted evolution in Acer acts rapidly.

  6. Ethnoveterinary medicines used to treat endoparasites and stomach problems in pigs and pets in British Columbia, Canada.

    Science.gov (United States)

    Lans, Cheryl; Turner, Nancy; Khan, Tonya; Brauer, Gerhard

    2007-09-30

    This paper documents the medicinal plants used to treat endoparasites and stomach problems in dogs, cats and pigs in British Columbia, Canada. Ethnoveterinary data was collected over a 6-month period in 2003. The majority of the information on pets came from 2 naturopaths, 10 herbalists, 5 dog trainers, breeders and pet shop owners, 9 holistic veterinarians and 6 of 27 organic farmers. Two pig farmers joined the study in the final stages. The following plants were used as anthelmintics: Artemisia cina O. Berg and C.F. Schmidt, Artemisia vulgaris L., Artemisia annua, Calendula officinalis L., Echinacea purpurea (L.) Moench (all Asteraceae), Mentha piperita L. and Salvia officinalis L. (Lamiaceae) (Allium sativum L. (Alliaceae), Cucurbita pepo L. (Cucurbitaceae), Eugenia caryophyllata Thunb (Myrtaceae), Gentiana lutea L. (Gentianaceae), Hydrastis canadensis L. (Ranunculaceae), Juglans nigra L. (Juglandaceae), Olea europaea L. (Oleaceae) and Ruta graveolens L. (Rutaceae)). Stomach problems were treated with: Achillea millefolium L. (Asteraceae), Aloe vera (L.) Burm. f. (Asphodelaceae), Elytrigia repens (L.) Desv. ex Nevski (Poaceae), Frangula purshiana (DC.) Cooper (Rhamnaceae), Juniperus communis L. (Cupressaceae), Melissa officinalis L. and M. piperita L. (Lamiaceae), Petroselinum crispum L. (Apiaceae), Plantago major L. and Plantago ovata Forssk. (Plantaginaceae) Rumex crispus L. and Rumex obtusifolius L. (Polygonaceae), Ulmus fulva Michx. (Ulmaceae) and Zingiber officinalis Roscoe (Zingiberaceae). There is insufficient information available to assess the anthelmintic efficacies of C. officinalis, Salvia officinalis, Eugenia caryophyllata and O. europaea; the other plants have mid- to high-level validity for their ethnoveterinary uses.

  7. Ethnobotanical survey of medicinal plants marketed in the municipality of Uruará, Pará, Brazil

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    Reinaldo Lucas Cajaiba

    2016-02-01

    Full Text Available The present study aimed to perform an ethnobotanical survey of medicinal plants marketed by the population of the municipality of Uruará, Pará, and its main districts. The respondents mentioned 63 species distributed in 36 botanical families. The most representative families were Lamiaceae, Asteraceae, Fabaceae and Rutaceae. The species with the highest Use Value were capim cidreira (Cymbopogon citratus, UV = 0.90, mastruz (Chenopodium ambrosioides, UV = 0.83 and hortelã (Mentha sp., UV = 0.79, while capim cidreira (Cymbopogon citratus and picão (Bidens pilosa had a higher indicator value, and were indicated as a tranquilizer/painkiller and for treating kidney disease, respectively. Among the main disorders treated with medicinal plants, diseases of the digestive system, infections/inflammations, colds and respiratory system diseases were the most cited. There was no significant difference between the number of species mentioned and the number of individuals per family or the distance of households to the urban zone. There was also no difference between the number of species mentioned and education level. Most medicinal plants marketed in the municipality are herbs, leaves are the most used parts and the most common form of preparation is tea.

  8. Ethnobotanical survey of medicinal plants marketed in the municipality of Uruará, Pará, Brazil

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    Reinaldo Lucas Cajaiba

    2016-02-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2016v29n1p115 The present study aimed to perform an ethnobotanical survey of medicinal plants marketed by the population of the municipality of Uruará, Pará, and its main districts. The respondents mentioned 63 species distributed in 36 botanical families. The most representative families were Lamiaceae, Asteraceae, Fabaceae and Rutaceae. The species with the highest Use Value were capim cidreira (Cymbopogon citratus, UV = 0.90, mastruz (Chenopodium ambrosioides, UV = 0.83 and hortelã (Mentha sp., UV = 0.79, while capim cidreira (Cymbopogon citratus and picão (Bidens pilosa had a higher indicator value, and were indicated as a tranquilizer/painkiller and for treating kidney disease, respectively. Among the main disorders treated with medicinal plants, diseases of the digestive system, infections/inflammations, colds and respiratory system diseases were the most cited. There was no significant difference between the number of species mentioned and the number of individuals per family or the distance of households to the urban zone. There was also no difference between the number of species mentioned and education level. Most medicinal plants marketed in the municipality are herbs, leaves are the most used parts and the most common form of preparation is tea.

  9. In vitro and in vivo antifungal activity, liver profile test, and mutagenic activity of five plants used in traditional Mexican medicine

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    Jorge Cornejo-Garrido

    Full Text Available Berberis hartwegii Benth., Berberidaceae, Hamelia patens Jacq., Rubiaceae, Dendropanax arboreus (L. Decne & Planch., Araliaceae, Erythrina herbacea L., Fabaceae, and Zanthoxylum caribaeum Lam., Rutaceae, acetone extracts were selected on the basis of their use in traditional Mexican medicine to treat scabies or skin diseases. Anti-dermatophyte activity in vitro was evaluated using the agar dilution assay, and the therapeutic efficacy of B. hartwegii and Z. caribaeum were tested against experimental tinea pedis. The infected animals were treated intragastrically daily for seven days with 2.5 and 5 mg/kg of acetone extracts. The acetone extract of H. patens exhibited 100% growth inhibition against T. mentagrophytes and E. floccosum at 100.0 and 50.0 µg/ml, respectively, and B. hartwegii inhibited growth of M. canis and T. mentagrophytes at 100.0 µg/ml. Effective treatments with 2.5 mg/kg of Z. caribaeum and B. hartwegii extract were comparable with 1 mg/kg of clotrimazole in mice. Liver profile tests and histological analyses did not exhibit any signs of toxicity and the Ames test indicated that both extracts were safe when evaluated in strains TA98, TA100 and TA102. Our results suggest the potential for the future development of new antifungal drugs from B. hartwegii or Z. caribaeum.

  10. Analysis of E. rutaecarpa Alkaloids Constituents In Vitro and In Vivo by UPLC-Q-TOF-MS Combined with Diagnostic Fragment

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    Shenshen Yang

    2016-01-01

    Full Text Available Evodia rutaecarpa (Juss. Benth. (Rutaceae dried ripe fruit is used for dispelling colds, soothing liver, and analgesia. Pharmacological research has proved that alkaloids are the main active ingredients of E. rutaecarpa. This study aimed to rapidly classify and identify the alkaloids constituents of E. rutaecarpa by using UPLC-Q-TOF-MS coupled with diagnostic fragments. Furthermore, the effects of the material base of E. rutaecarpa bioactive ingredients in vivo were examined such that the transitional components in the blood of rats intragastrically given E. rutaecarpa were analyzed and identified. In this study, the type of alcohol extraction of E. rutaecarpa and the corresponding blood sample were used for the analysis by UPLC-Q-TOF-MS in positive ion mode. After reviewing much of the literature and collected information on the fragments, we obtained some diagnostic fragments of the alkaloids. Combining the diagnostic fragments with the technology of UPLC-Q-TOF-MS, we identified the compounds of E. rutaecarpa and blood samples and compared the ion fragment information with that of the alkaloids in E. rutaecarpa. A total of 17 alkaloids components and 6 blood components were identified. The proposed method was rapid, accurate, and sensitive. Therefore, this technique can reliably and practically analyze the chemical constituents in traditional Chinese medicine (TCM.

  11. The antiplasmodium effects of a traditional South American remedy: Zanthoxylum chiloperone var. angustifolium against chloroquine resistant and chloroquine sensitive strains of Plasmodium falciparum

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    Gerardo Cebrian-Torrejon

    2011-06-01

    Full Text Available Zanthoxylum chiloperone var. angustifolium Engl., Rutaceae, is used in traditional medicine to treat fungal and protozoal infections in the central area of South America. Considering the increasing resistance of Plasmodium falciparum in malarial ridden areas, we explored the anti-plasmodial effects of three compounds isolated from Z. chiloperone. The pyranocoumarin transavicennol and the canthinone alkaloids, canthin-6-one and 5-methoxycanthin-6-one, were found to have IC50 on chloroquine/mefloquine resistant and sensitive strains of P. falciparum of 0.5-2.7, 2.0-5.3 and 5.1-10.4 ƒÊg/mL, respectively. Moreover, the formation of heme adducts by these compounds is described by a novel alternative method based on MS-CID methods. The alkylamide sanshool was also identified, for first time in this plant, in the dichloromethanic and ethanolic extracts and the extracts were found to be notably non-toxic and displayed good anti-plasmodial effects.

  12. Análise florística e estrutural de um fragmento de floresta ombrófila mista montana, situado em Criúva, RS – Brasil

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    Rubens Neto Marques Rondon

    2002-01-01

    Full Text Available The aim of this paper was to know and analyze the floristic composition and describe the structure of the arboreous component of a fragment of Montane Mixed Ombrophylous Forest in Cri úva – State of Rio Grande do Sul, Brazil. Were inventoried 673 individuous with DBH ³ 5 cm eight 10 x 100 m plots, distributed into 37 species, 32 genera and 22 families. The Shannon diversity index was 2,768. The families which presented the largest number of individuous were: Araucariaceae, Myrsinaceae, Euphorbiaceae, Anacardiaceae and Rutaceae. The forest canopy is dominated by Araucaria angustifolia and the understory, by species of the families Lauraceae and Myrtaceae. The total density found was 841,25 individuous/ha, the mean DBH was 24,01 cm and the highest VI values, in descending order, were: Araucaria angustifolia, Sebastiania commersoniana , Lithraea brasiliensis, Zanthoxylum rhoifolium and Myrcia sp.. The fragment presents a considerable floristic diversity of species, in spite of the suffered disturbances in the past. For the vegetation to reach the floristic and structural characteristics close to the one of the original vegetation it is necessary to avoid the presence of bovine and equine in the area and explorations of the forest.

  13. Evaluation of Anti-Inflammatory Activity of Citrus latifolia Tanaka Essential Oil and Limonene in Experimental Mouse Models

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    Raquel Kummer

    2013-01-01

    Full Text Available The genus Citrus (Rutaceae includes several species of plants that produce some of the most cultivated fruits in the world, providing an appreciable content of essential oil. In folk medicine, they are used as a cholagogue, antipyretic, anti-inflammatory, sedative, and antitoxic effects. Lemon essential oil has been used since ancient times for its antiseptic, carminative, diuretic, and eupeptic effects. In this study, we investigated the anti-inflammatory activity of Citrus latifolia Tanaka essential oil (CLEO and its main constituent LIM. In the cell viability assay, CLEO and LIM (3, 10, 30, and 90 μg/mL had low cytotoxicity. In zymosan-induced peritonitis, LIM (500 mg/kg decreased the infiltration of peritoneal exudate leukocytes and decreased the number of polymorphonuclear leukocytes. In vitro chemotaxis revealed that CLEO and LIM (1, 3, and 10 µg/mL promoted a significant reduction of neutrophil migration toward fMLP and LTB4. LIM (500 mg/kg also reduced TNF-α levels but did not alter IL-10 levels in the peritoneal exudate. In conclusion, this study showed that LIM isolated from CLEO had potential anti-inflammatory effects, likely by inhibiting proinflammatory mediators present in inflammatory exudate and leukocyte chemotaxis.

  14. Phytossociology of wood community in Seasonal Dry Montane Forest in Paraiba, Brazil

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    Maria do Carmo Learth Cunha

    2013-06-01

    Full Text Available The Pico do Jabre Seasonally Dry Montane Forest in Paraiba state, Brazil, the highest regional elevation, 1197 m, distant 360 km the sea was assessed aiming to survey its phytosociology and woody structure. In 36 systematic sampling plots, 10x50m, individuals, Dbh > 4.8cm, had their diameters and height measured. Botanical samples were collected during five years and vouchers were deposited at the Paraiba Federal University Herbaria (JPB. It was found 2050 trees distributed in 64 species of 51 genera of 31 families, which accounted for 1138 ind.ha-1 and 22.45 m2.ha -1. Diversity and equability were assessed as H' = 3.17 nats.ind-1 and J' = 0.76 similar to some others regional seasonally dry montane forest communities. Malpighiaceae, Myrtaceae, Erythroxylaceae, Vochysiaceae, Celastraceae, Rutaceae, Sapindaceae e Fabaceae-Faboideae stood out and summed 66.72% of the total VI. Byrsonima nitidifolia, Eugenia ligustrina, Calisthene microphylla, Maytenus distichophylla and Erythroxylum mucronatum species accounted for 120.79 (40.3% of the total VI. B. nitidifolia ecological dominance is firstly reported in the Brazilian northeast region.

  15. Citrus tristeza virus (CTV influence on the behavior of Tahiti lime (Citrus latifoliaTanaka grafted on six rootstocks in the plain piedmont of Colombia (1997-2008

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    Hans Nicolás Chaparro Zambrano

    2013-01-01

    Full Text Available Tahiti lime, Citrus latifolia Tanaka (Rutaceae, is susceptible to Citrus Tristeza Virus (CTV, and if affected, decreases the profitability of the crop. In the well-drained high terrace of the Meta piedmont, the influence of the virus attack was evaluated on the performance of Tahiti lime grafted on six rootstocks (Carrizo; Sunki × English; Sunki × Jacobson; Pomeroy; Rubidoux, y Kryder 15-3 on a completely randomized experiment design taking each tree as an experimental unit. A comparison of means and inferential statistic were used for: plant height, height and canopy diameter; canopy volume, yield and severity of CTV. All rootstocks showed homogenous height. Carrizo was the exception with less size and canopy volume. The highest accumulated fruit yield (Kg/tree wasobtained by Sunki x English, followed by Rubidoux and the lowest yield was obtained by Carrizo. The highest yield efficiency of average canopy volume was obtained with Sunki x Jacobson. The Tahiti lime yield and morphological development fell in the seventh year (2007 in all rootstocks, due to an infection caused by a mix of isolates of CTV type Madeira and type B128, isolates which cause stem channeling : type B31 which causes moderate stem channeling and B7 and VT isolate,

  16. Sex allocation and functional bias of quaternary and quinary flowers on same inflorescence in the hermaphrodite Ruta graveolens

    Science.gov (United States)

    Tang, Jing-Yu; Ren, Ming-Xun

    2011-09-01

    Intra-inflorescence variation in floral traits is important to understand the pollination function of an inflorescence and the real reproductive outputs of a plant. Ruta graveolens (Rutaceae) produce both quaternary (four petals and eight stamens) and quinary (five petals and ten stamens) flowers on the same cymes, while their pollination roles and the effects on the reproductive success remained unexplored. We experimentally examined the biomass of female versus male organs and pollen viability and stigma receptivity to explore the sex allocation patterns between the flowers. The breeding systems and reproductive outputs through either female function (seed set) or male function (pollen dispersal) were also studied for quinary and quaternary flowers to determine whether there was functional bias. The results showed that R. graveolens was protandrous, with a mixed mating system. Its stamens could slowly move one by one and only dehisce when positioning at the flower center, which could greatly enhance pollen dispersal. The first-opened quinary flower allocated significantly higher resources (dry biomass) in female organs while quaternary flowers allocated more resource in male organs. The quaternary flowers experienced higher pollen limitation in seed production but were more successful in pollen dispersal and the quinary flowers reproduced both through female and male functions. Our data suggested that quinary and quaternary flower on same inflorescence in R. graveolens functioned mainly as the sex role that most resources were allocated, which probably reflect an adaptation for floral phenology and pollination process in this plant.

  17. Evaluation of Effects of Zingiber officinale on Salivation in Rats

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    Goli Chamani

    2011-06-01

    Full Text Available There are some herbal plants in Iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. The aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. The extracts of 7 herbs; Zingiber officinale Roscoe (Zingiberaceae, Citrus sinensis (L. Osbeck (Rutaceae, Artemisia absinthium L. (Asteraceae, Cichorium intybus L. (Asteraceae, Pimpinella anisum L.(Apiaceae, Portulaca oleracea L.(Portulacaceae, Tribulus terrestris L. (Zygophyllaceae were prepared. Nine groups of animals (including negative and positive control groups were used and seven rats were tested in each group. After the injection of extracts, saliva volume was measured gravimetrically in four continuous seven-minute intervals. The results showed that after injection of ginger extracts salivation was significantly higher as compared to the negative control group and other herbal extracts in all of the four intervals (P<0.01. The peak action of the ginger was during the first 7-minute interval and following this, salivation decreased to some extent. The present study suggests that the extract of Zingiber offiicianle can increase the rate of salivation significantly in animal model. Further investigations on different constituents of ginger seem to be essential to identify the responsible constituent for stimulation of saliva secretion.

  18. Acetylcholinesterase inhibition by somes promising Brazilian medicinal plants

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    CM. Feitosa

    Full Text Available A microplate assay and a thin-layer chromatography (TLC "in situ" assay based on the Ellman assay was used to screen for acetylcholinesterase inhibitors from ethyl acetate and methanol extracts of Brazilian medicinal plants of families that, according to the literature, have traditional uses that might be connected with acetylcholinesterase inhibition. Eighteen species belonging to Convolvulaceae, Crassulaceae, Euphorbiaceae, Leguminosae, Malvaceae, Moraceae, Nyctaginaceae and Rutaceae families were tested. The most active plants were Ipomoea asarifolia (IC50 = 0.12 mg/mL, Jatropha curcas (IC50 = 0.25 mg/mL, Jatropha gossypiifolia (IC50 = 0.05 mg/mL, Kalanchoe brasiliensis (IC50 = 0.16 mg/mL and Senna alata (IC50 = 0.08 mg/mL. The most promising extracts were the Jatropha gossypiifolia and Senna alata species assuming there were compounds with a similar activity to galanthamine, which should contain about 1% of an active compound, or if present at lower levels even more active compounds than galanthamine (IC50 = 0.37 x 10-3 mg/mL should be present.

  19. Antinociceptive esters of N-methylanthranilic acid: Mechanism of action in heat-mediated pain.

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    Pinheiro, Mariana Martins Gomes; Radulović, Niko S; Miltojević, Ana B; Boylan, Fabio; Dias Fernandes, Patrícia

    2014-03-15

    Recently, we identified a new natural antinociceptive alkaloid ternanthranin, isopropyl N-methylanthranilate (ISOAN), from the plant species Choisya ternata Kunth (Rutaceae). In this work we concentrated on the elucidation of its mechanism of action in comparison with two other esters of this acid (methyl (MAN) and propyl (PAN)). Mice orally pre-treated with ISOAN, MAN or PAN (at 0.3, 1 and 3mg/kg) were less sensitive to chemical or thermal stimuli in different nociception models (formalin-, capsaicin- and glutamate-induced licking response, tail flick and hot plate). All compounds (1 and 3mg/kg) showed significant activity in the peripheral nociception models, as well as a dose-dependent spinal antinociceptive effect in the tail flick model. We observed that glibenclamide was able to reverse the antinociceptive effect of ISOAN in the hot plate model suggesting the involvement of K(+)ATP channels. The antinociceptive effect of MAN and PAN may be related to adrenergic, nitrergic and serotoninergic pathways. In addition, the antinociception of PAN was reverted by naloxone implying that the opioid pathway participates in its activity. The cholinergic and cannabinoid systems were found not be involved in the onset of the antinociceptive effects of any of the esters. In conclusion, isopropyl, methyl and propyl N-methylanthranilates produced significant peripheral and central antinociception at doses lower than that of morphine, the classical opioid analgesic drug, without causing toxicity. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. Late Holocene paleoenvironments of the floodplain of the Solimões River, Central Amazonia, based on the palynological record of Lake Cabaliana

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    Natália de Paula Sá

    Full Text Available ABSTRACT The core PD-67 of 160 cm depth was collected from the delta of Lake Cabaliana situated on the Solimões River. Seventeen samples were removed for palynological and sedimentological analysis and three for radiocarbon analysis. Two dry periods, both in the Late Holocene, were observed (2800-2550 cal yr BP, 1450-550 cal yr BP separated by a wetter phase (2550-1450 cal yr BP. In 2800-2550 cal yr BP, varzea forests of Alchornea, Symmeria, Cecropia, Alternanthera and Asteraceae were predominant. Beginning in 2,550-1450 cal yr BP, the varzea was characterized by pioneer elements, such as Cassia, Laetia, Mabea, Symmeria and Cecropia, and by the expansion of Poaceae, Cyperaceae, Sagittaria, Montrichardia and Asteraceae. In 1450-550 cal yr BP the succession of varzea continued with Pseudobombax, Laetia, Luehea/Lueheopsis and Ryanaea increasing simultaneously with the terra firme vegetation of Rutaceae, Sapotaceae, Styrax, Scleronema, Anthurium, Araceae, pteridophytes and Pariana. The successional dynamics at Lake Cabaliana indicated that the local varzea had become established recently, and is composed of a mosaic of different successional stages of vegetation influenced mainly by flood pulse and variation in rainfall. It is therefore possible to propose that the recent climate history of Central Amazonia reflects changes in rainfall patterns in the basin.

  1. Taxonomy and phylogeny of the genus citrus based on the nuclear ribosomal dna its region sequence

    International Nuclear Information System (INIS)

    Sun, Y.L.

    2015-01-01

    The genus Citrus (Aurantioideae, Rutaceae) is the sole source of the citrus fruits of commerce showing high economic values. In this study, the taxonomy and phylogeny of Citrus species is evaluated using sequence analysis of the ITS region of nrDNA. This study is based on 26 plants materials belonging to 22 Citrus species having wild, domesticated, and cultivated species. Through DNA alignment of the ITS sequence, ITS1 and ITS2 regions showed relatively high variations of sequence length and nucleotide among these Citrus species. According to previous six-tribe discrimination theory by Swingle and Reece, the grouping in our ITS phylogenetic tree reconstructed by ITS sequences was not related to tribe discrimination but species discrimination. However, the molecular analysis could provide more information on citrus taxonomy. Combined with ITS sequences of other subgenera in then true citrus fruit tree group, the ITS phylogenetic tree indicated subgenera Citrus was monophyletic and nearer to Fortunella, Poncirus, and Clymenia compared to Microcitrus and Eremocitrus. Abundant sequence variations of the ITS region shown in this study would help species identification and tribe differentiation of the genus Citrus. (author)

  2. The antibacterial and antifungal activity of essential oils extracted from Guatemalan medicinal plants.

    Science.gov (United States)

    Miller, Andrew B; Cates, Rex G; Lawrence, Michael; Soria, J Alfonso Fuentes; Espinoza, Luis V; Martinez, Jose Vicente; Arbizú, Dany A

    2015-04-01

    Essential oils are prevalent in many medicinal plants used for oral hygiene and treatment of diseases. Medicinal plant species were extracted to determine the essential oil content. Those producing sufficient oil were screened for activity against Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. Plant samples were collected, frozen, and essential oils were extracted by steam distillation. Minimum inhibitory concentrations (MIC) were determined using a tube dilution assay for those species yielding sufficient oil. Fifty-nine of the 141 plant species produced sufficient oil for collection and 12 species not previously reported to produce essential oils were identified. Essential oil extracts from 32 species exhibited activity against one or more microbes. Oils from eight species were highly inhibitory to S. mutans, four species were highly inhibitory to C. albicans, and 19 species yielded MIC values less than the reference drugs. RESULTS suggest that 11 species were highly inhibitory to the microbes tested and merit further investigation. Oils from Cinnamomum zeylanicum Blume (Lauraceae), Citrus aurantiifolia (Christm.) Swingle (Rutaceae), Lippia graveolens Kunth (Verbenaceae), and Origanum vulgare L. (Lamiaceae) yielded highly significant or moderate activity against all microbes and have potential as antimicrobial agents. Teas prepared by decoction or infusion are known methods for extracting essential oils. Oils from 11 species were highly active against the microbes tested and merit investigation as to their potential for addressing health-related issues and in oral hygiene.

  3. Análise econômica de sistemas agroflorestais na Amazônia ocidental, Machadinho d'Oeste- RO Economic analysis of agroforestry systems in eastern Amazonia, Machadinho d'Oeste- RO, Brazil

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    Michelliny de Matos Bentes-Gama

    2005-06-01

    Full Text Available O objetivo deste estudo foi realizar a análise financeira e a simulação de risco de investimento em sistemas agroflorestais (SAFs implantados em 1987, no Campo Experimental da Embrapa Rondônia, localizado no município de Machadinho d'Oeste, RO. A análise financeira foi realizada mediante os métodos de avaliação de projetos florestais, e para a análise de risco utilizou-se a técnica de simulação de Monte Carlo, mediante o programa @RISK. Entre os arranjos testados, o SAF T1 Castanha-do-brasil-banana-pimenta-do-reino-cupuaçu apresentou o melhor desempenho financeiro em relação aos SAFs T2 Freijó-banana-pimenta-do-reino-cupuaçu e T3 Pupunha-banana-pimenta-do-reino-cupuaçu . Os custos com tratos culturais e colheita representaram mais de 70% da composição dos custos totais, e a participação da mão-de-obra foi superior a 50% nas fases de preparo da área e de manutenção (tratos culturais dos SAFs. A simulação da análise de risco indicou que as variáveis que afetaram o Valor Presente Líquido no Horizonte Infinito (VPL*, de acordo com a ordem de importância (R, foram: taxa de desconto, preço do fruto de cupuaçu (Theobroma grandiflorum, custo de colheita, preço da madeira de castanha-do-brasil (Bertholletia excelsa e o custo de tratos culturais. Apesar do alto custo de implantação e manutenção, o SAF T1 apresentou uma probabilidade de 15% de os valores do Valor Presente Líquido (VPL se concentrarem em torno de R$35.000 ha-1.ano-1.The objective of this study was to carry out the financial analysis and the risk of investment simulation in agroforestry systems (AFSs established in 1987 in the Experimental Field of Embrapa Rondônia, located in the County of Machadinho d'Oeste, RO. Financial analysis was made through the evaluation of results from the forestry enterprise evaluation methods, and the risk analysis was carried out through the Monte Carlo simulation technique by @RISK software. Among the arrangements

  4. Prospeção de inibidores de serinoproteinases em folhas de leguminosas arbóreas da floresta Amazônica Prospecting serine proteinase inhibitors in leaves from leguminous trees of the Amazon forest

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    Larissa Ramos Chevreuil

    2011-03-01

    Full Text Available Os inibidores de proteinases são proteínas extensivamente investigadas nos tecidos de estocagem, mas pouco prospectadas em outros tecidos vegetais. O objetivo deste estudo foi detectar a presença de inibidores de serinoproteinases em extratos foliares de quinze espécies de leguminosas arbóreas da Amazônia. As espécies estudadas foram: Caesalpinia echinata, C. ferrea, Cedrelinga cateniformis, Copaifera multijuga, Dinizia excelsa, Enterolobium contortisiliquum, E. maximum, E. schomburgkii, Leucaena leucocephala, Ormosia paraensis, Parkia multijuga, P. pendula, P. platycephala, Swartzia corrugata e S. polyphylla. Folhas foram coletadas, secas a 30ºC durante 48 h, trituradas e submetidas à extração com NaCl (0,15 M, 10% p/v resultando no extrato total. Ensaios foram executados para determinar a concentração de proteínas e detectar a atividade inibitória contra a tripsina e quimotripsina bovina. Os teores de proteínas bruta e solúvel nos extratos foliares variaram de 7,9 a 31,2% e 1,3 a 14,8%, respectivamente. A atividade inibitória sobre a tripsina e quimotripsina foi observada em todos os extratos foliares. Contudo, nos extratos de E. maximum, L. leucocephala, P. pendula, S. corrugata e S. polyphylla a inibição foi maior sobre a tripsina, enquanto o extrato de P. multijuga foi mais efetivo contra a quimotripsina. Nós concluímos que nos extratos foliares de leguminosas arbóreas têm inibidores de serinoproteinases e exibem potencial aplicações biotecnológicas.The proteinase inhibitors are proteins extensively investigated in tissue storage, but few prospected in other plant tissues. The aim of this study was to detect the presence of serine proteinase inhibitors in leaf extracts from fifteen species of leguminous trees of the Amazon forest. The species studied were Caesalpinia echinata, C. ferrea, Cedrelinga cateniformis, Copaifera multijuga, Dinizia excelsa, Enterolobium contortisiliquum, E. maximum, E. schomburgkii

  5. Áreas prioritárias na Amazônia para conservação dos recursos genéticos de espécies florestais nativas: fase preliminar Priority areas for genetic resources conservation of native Forest Species: preliminary phase

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    Angela Maria Conte Leite

    1993-07-01

    Full Text Available O CENARGEN/EMBRAPA está realizando a prospecção das áreas prioritárias para a conservação in situ dos recursos genéticos de espécies com importância sócio-econômica para cada bioma existente no Brasil, buscando a conservação da variabilidade genética das mesmas. Para a prospecção da floresta tropical úmida amazônica, inicialmente foram feitos levantamentos de herbários nacionais bem como de literatura, para tentar definir os padrões de distribuição geográfica de algumas espécies prioritárias da região. A ocorrência das mesmas foi correlacionada com solos, clima, vegetação e com a existência de unidades de conservação na Amazônia, no intuito de se propor as áreas mais adequadas para a implantação de reservas genéticas (conservação in situ e de bancos de germoplasma (conservação ex situ à campo. As espécies relatadas no presente trabalho são Aniba rosaeodora Ducke (pau-rosa, Bertholletia excelsa H.B.K. (castanha-do-Pará, Carapa quianensis Aubl. (andiroba, Cedrela fissilis Veil, e C. odorata L. (cedros, Copaifera langsdorffii Desf. e C. multijuga Hayne (copaibas, Cordia goeldiana Huber (freijó, Jacaranda copaia (Aubl. D. Dom (pará-pará, Simaruba amara Aubl. e Virola sebifera Aubl. e V. surinamensis Warb. (ucuúbas e Voucapoua americana Aubl. e V. pallidor Ducke (acapús. Trabalhos posteriores com as mesmas incluirão a corroboração à campo dos dados obtidos nos herbários e na literatura, bem como estudos a nível de população dentro das áreas selecionadas.CENARGEN/EMBRAPA is investigating priority areas for in situ conservation of genetic resources of socially and economically important species. Herbaria and the literature were consulted to determine the distribution patterns of some tropical rain forest species. Known species distribution were correlated with soils, climate, vegetation types and the existence of conservation units, in order to propose the establishment of genetic reserves for

  6. Morphometric of four species in agroforestry systems in the municipality of Porto Velho, Rondônia. = Morfometria de quatro espécies florestais em sistemas agroflorestais no munícipio de Porto Velho, Rondônia

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    Tiago Monteiro Condé

    2013-04-01

    Full Text Available This study aims to describe the morphometry of Andiroba (Carapa guianensis Aubl, Brasil nut tree (Bertholletia excelsa HBK, Copaíba (Copaifera langsdorffii Desf. Mogno (Swietenia macrophylla King. and simulate the vital space for it to grow without competition in agroforestry plantations. Data were collected in 20 agroforestry (SAF’s considered productive age (16.5 years of Project RECA (Economic Reforestation Consortium and Compacted, the municipality of Porto Velho, Rondônia. The deployment of the SAF’s occurred through the removal of vegetation through the process of clearing and burningof native forests. Morphometric data were collected randomly from individuals with DBH > 10 cm of four tree species in onehectare of each farm, for a total sample area of 20 ha (20 properties. The sample was composed by 25 individuals of Andiroba,40 of Brasil nut tree, 23 of Copaíba and 46 of Mogno. Andiroba was very similar to Mogno in relation to Cup Percentage (PC,Coverage Index (AI and Cup Form (FC, both showed potential for higher density plantations with timber purposes. The Brasilnut tree and Copaíba had the highest values of the FC (1.66, 1.79 and Crown Area (92.60, 57.51, respectively, showing frondscrowns, silvicultural interesting features for the extraction of seeds. Was simulated living space for the four species developwithout competition between cups. It was concluded that the four species have potential for planting for extraction of timberand non-timber forest in areas subject to degradation and deforestation in the Amazon.ResumoO presente estudo visa descrever a morfometria das espécies Andiroba (Carapa guianensis Aubl, Castanheira-do-Brasil (Bertholletia excelsa H.B.K., Copaíba (Copaifera langsdorffii Desf. e Mogno (Swietenia macrophylla King. e simular o espaço vital para que as mesmas cresçam sem concorrência em plantios agroflorestais. Os dados foram coletadosem vinte sistemas agroflorestais (SAF’s em idade considerada

  7. Soy and Brazil nut beverage: processing, composition, sensory, and color evaluation Bebida de soja e castanha do Brasil: processamento, composição, avaliação sensorial e de cor

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    Ilana Felberg

    2009-09-01

    Full Text Available Despite its high nutritional value, soymilk consumption in Western countries is limited mainly due to undesirable flavors developed during the traditional elaboration process. Brazil nut (Bertholletia excelsa has pleasant flavor and recognized nutritional value. Thus, the aim of this work was to elaborate a soy and Brazil nut beverage exploring the use of two national products of high nutritional quality. The process for manufacturing a soy and Brazil nut beverage consisted of elaboration, formulation, and homogenization of soymilk and Brazil nut milk. The addition of five levels (10, 20, 30, 40, and 50% of Brazil nut milk to soy beverages was investigated. Although no significant differences in consumer average preference (p > 0.05 were observed among the beverages, analyzing both the consumer preference frequency distribution of the products and the Internal Preference Mapping (IPM, it was possible to conclude that the beverage with 30% of Brazil nut milk reached the most adequate performance demonstrating the sensory benefits Brazil nuts brought to the product. Regarding proximate composition, it did not present a better performance in terms of nutritional value, except for the oil content. The soy and Brazil nut beverage presented visual stability and no phase separation despite the non-stability shown by Brazil nut beverage itself. When Brazil nut milk was added to soy beverage, the final product became whiter than soy beverage, which is appealing to consumers who normally search for a clearer soymilk. The soy and Brazil nut beverage processing can be considered an alternative to increase the use of Brazil nuts in the Brazilian diet.Apesar do alto valor nutricional do extrato de soja, seu consumo nos países do ocidente ainda é limitado principalmente devido às características sensoriais desagradáveis resultantes do seu tradicional processo de obtenção. A castanha do Brasil (Bertholletia excelsa apresenta sabor agradável e

  8. Study of the specific concentrations of {sup 40}K, {sup 224}Ra, {sup 226}Ra and {sup 228}Ra in some seasonings marketed in Rio de Janeiro City

    Energy Technology Data Exchange (ETDEWEB)

    Garcêz, Ricardo W.D.; Lopes, José M.; Silva, Leandro B.; Silva, Ademir X. da [Coordenacao de Pos-Graduacao e Pesquisa de Engenharia (PEN/COPPE/UFRJ), Rio de Janeiro, RJ (Brazil). Programa de Engenharia Nuclear; Lima, Marco A.F., E-mail: rgarcez@nuclear.ufrj.br [Universidade Federal Fluminense (UFF), Niterói, RJ (Brazil). Instituto de Biologia

    2017-07-01

    The seasoning are vegetables substances used in foods to enhance their flavor, aroma and color. This work presents an investigation of the activity concentration of Naturally Occurring Radioactive Materials (NORMs) in 28 samples of seasoning utilized by brazilian population. The seasoning samples were measured using gamma spectroscopy technique with a high-purity germanium (HPGe) detector, a non-destructive nuclear method and with the LabSOCS software for the calculation of the efficiency curve. The analysis shows that the activity concentration of {sup 40}K was measured in all samples and ranges from 21.0 Bq/kg to 1288 Bq/kg. The highest concentration activity of {sup 40}K was measured to 'cheiro verde', a local seasoning made of chives (Allium Schoenoprasum) and parsley (Petroselinum Crispum), while annatto, made with the fruit of Bixa Orelhana, had the lowest activity concentration. Brazil nut (Bertholletia Excelsa) presented the highest concentrations for {sup 226}Ra and {sup 228}Ra with 24 Bq/kg and 25.7 Bq/kg, respectively and black pepper (Piper Nigrum) presented the highest concentration for {sup 224}Ra with 33.9 Bq/kg. The highest effective dose for members of the public due to ingestion was 23.5 μSv/y due to Brazil nut and the lowest effective dose was found for annatto: 0.13 μSv/y. The syrian seasoning sample present specific concentration of 6.1±1.1 Bq/kg for {sup 137}Cs and 0.08 μSv/y of effective dose. The values found in this work do not represent a risk to human health. (author)

  9. ¿Qué es el humanismo? Problemática de la formación humanística

    Directory of Open Access Journals (Sweden)

    Eudoro Rodríguez Albarracín

    2008-01-01

    Full Text Available Humanismo es un término polisémico sujeto a diversas formas de interpretación y realización. En sentido genérico, se dice humanista a cualquier doctrina que arme la excelsa dignidad humana, el carácter racional y de n del hombre, que enfatiza su autonomía, su libertad y su capacidad de transformación de la historia y la sociedad. En sentido histórico, el humanismo se plasmó originariamente en la cultura grecorromana, convirtiéndose sus realizaciones culturales y sus ideales educativos, como insuperables y normativos, para el pensamien - to y cultura de los individuos. Movimiento histórico que se constituyó además en una tradición de la cultura occidental, asumida por la Patrísti - ca y redescubierta por la Escolástica, especialmente en las obras de San Agustín y Santo Tomás de Aquino. Desde el siglo XV, se conguró como un movimiento global cultural de rescate y sobre dimensionamiento de la cultura clásica, especialmente en Italia y luego en la mayoría de los países europeos. En sentido paradigmático, el humanismo se expresa en diversas losofías y visiones del hombre contrapuestas (humanismo cris - tiano, marxista, existencialista, personalista, etc. que enfatiza el carácter antropocéntrico de la cultura moderna. Dicha tradición y patrimonio, se ve hoy cuestionada por las losofías antihumanistas que le niegan su capacidad interpretativa y esclarecedora de la vida humana y de la sociedad. Subsisten sin embargo, desde la antigüedad las dos formas básicas de humanismo: el humanismo teocéntrico y el humanismo secularizado explícitamente ateo.

  10. Study of the specific concentrations of 40K, 224Ra, 226Ra and 228Ra in some seasonings marketed in Rio de Janeiro City

    International Nuclear Information System (INIS)

    Garcêz, Ricardo W.D.; Lopes, José M.; Silva, Leandro B.; Silva, Ademir X. da; Lima, Marco A.F.

    2017-01-01

    The seasoning are vegetables substances used in foods to enhance their flavor, aroma and color. This work presents an investigation of the activity concentration of Naturally Occurring Radioactive Materials (NORMs) in 28 samples of seasoning utilized by brazilian population. The seasoning samples were measured using gamma spectroscopy technique with a high-purity germanium (HPGe) detector, a non-destructive nuclear method and with the LabSOCS software for the calculation of the efficiency curve. The analysis shows that the activity concentration of 40 K was measured in all samples and ranges from 21.0 Bq/kg to 1288 Bq/kg. The highest concentration activity of 40 K was measured to 'cheiro verde', a local seasoning made of chives (Allium Schoenoprasum) and parsley (Petroselinum Crispum), while annatto, made with the fruit of Bixa Orelhana, had the lowest activity concentration. Brazil nut (Bertholletia Excelsa) presented the highest concentrations for 226 Ra and 228 Ra with 24 Bq/kg and 25.7 Bq/kg, respectively and black pepper (Piper Nigrum) presented the highest concentration for 224 Ra with 33.9 Bq/kg. The highest effective dose for members of the public due to ingestion was 23.5 μSv/y due to Brazil nut and the lowest effective dose was found for annatto: 0.13 μSv/y. The syrian seasoning sample present specific concentration of 6.1±1.1 Bq/kg for 137 Cs and 0.08 μSv/y of effective dose. The values found in this work do not represent a risk to human health. (author)

  11. Brazil nuts intake improves lipid profile, oxidative stress and microvascular function in obese adolescents: a randomized controlled trial

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    Koury Josely C

    2011-05-01

    Full Text Available Abstract Background Obesity is a chronic disease associated to an inflammatory process resulting in oxidative stress that leads to morpho-functional microvascular damage that could be improved by some dietary interventions. In this study, the intake of Brazil nuts (Bertholletia excelsa, composed of bioactive substances like selenium, α- e γ- tocopherol, folate and polyunsaturated fatty acids, have been investigated on antioxidant capacity, lipid and metabolic profiles and nutritive skin microcirculation in obese adolescents. Methods Obese female adolescents (n = 17, 15.4 ± 2.0 years and BMI of 35.6 ± 3.3 kg/m2, were randomized 1:1 in two groups with the diet supplemented either with Brazil nuts [BNG, n = 08, 15-25 g/day (equivalent to 3 to 5 units/day] or placebo [PG (lactose, n = 09, one capsule/day] and followed for 16 weeks. Anthropometry, metabolic-lipid profiles, oxidative stress and morphological (capillary diameters and functional [functional capillary density, red blood cell velocity (RBCV at baseline and peak (RBCVmax and time (TRBCVmax to reach it during post-occlusive reactive hyperemia, after 1 min arterial occlusion] microvascular variables were assessed by nailfold videocapillaroscopy at baseline (T0 and after intervention (T1. Results T0 characteristics were similar between groups. At T1, BNG (intra-group variation had increased selenium levels (p = 0.02, RBCV (p = 0.03 and RBCVmax (p = 0.03 and reduced total (TC (p = 0.02 and LDL-cholesterol (p = 0.02. Compared to PG, Brazil nuts intake reduced TC (p = 0.003, triglycerides (p = 0.05 and LDL-ox (p = 0.02 and increased RBCV (p = 0.03. Conclusion Brazil nuts intake improved the lipid profile and microvascular function in obese adolescents, possibly due to its high level of unsaturated fatty acids and bioactive substances. Trial Registration Clinical Trials.gov NCT00937599

  12. A Governança do Arranjo Produtivo Local da Castanha-do-Brasil na Região da Calha Norte, Pará

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    Márcia Nágem Krag

    Full Text Available Resumo: O objetivo do trabalho foi analisar as formas de governança do Arranjo Produtivo Local (APL da castanha-do-brasil (Berthollethia excelsa Bonpl. na região da Calha Norte, do estado do Pará, caracterizando os fatores específicos que condicionam a governança. Os dados foram obtidos por meio de questionário estruturado no padrão RedeSist, aplicados aos agentes da cadeia produtiva local, com ênfase nas empresas de beneficiamento de castanhas. A análise dos resultados foi desenvolvida com base nos índices de percepção dos entrevistados sobre a estrutura, a governança e as vantagens associadas ao ambiente local, fundamentada nas teorias das aglomerações produtivas, competitividade e governança. Os resultados mostram que pela grande concorrência existente entre as empresas, as transações comerciais realizadas localmente promovem internalização de conhecimento e aprendizagem de forma individual, não se desdobrando em geração de informação para o APL como um todo, em que os processos de cooperação e aprendizagem têm decorrido basicamente da experiência acumulada na produção e consequentes inovações incrementais em produtos e processos, caracterizando, assim, uma capacidade inovativa interna das empresas. Conclui-se que há baixa capacidade de gestão e governança nas ações cooperativas de forma sistêmica e, na ausência de estruturas de governança mais especializadas, predomina a governança de mercado no APL estudado.

  13. Determine the optimum spectral reflectance of juniper and pistachio in arid and semi-arid region

    Science.gov (United States)

    Fadaei, Hadi; Suzuki, Rikie

    2012-11-01

    Arid and semi-arid areas of northeast Iran cover about 3.4 million ha are populated by two main tree species, the broadleaf Pistacia vera. L (pistachio) and the conifer Juniperus excelsa ssp. polycarpos (Persian juniper). Natural stands of pistachio in Iran are not only environmentally important but genetically essential as seed sources for pistachio production in orchards. In this study, we estimated the optimum spectral reflectance of juniper forests and natural pistachio stands using remote sensing to help in the sustainable management and production of pistachio in Iran. In this research spectral reflectance are able to specify of multispectral from Advanced Land Observing Satellite (ALOS) that provided by JAXA. These data included PRISM is a panchromatic radiometer with a 2.5 m spatial resolution at nadir, has one band with a wavelength of 0.52-0.77 μm and AVNIR-2 is a visible and near infrared radiometer for observing land and coastal zones with a 10 m spatial resolution at nadir, has four multispectral bands: blue (0.42-0.50 μm), green (0.52-0.60 μm), red (0.61-0.69 μm), and near infrared (0.76-0.89 μm). Total ratio vegetation index (TRVI) of optimum spectral reflectance of juniper and pistachio have been evaluated. The result of TRVI for Pistachio and juniper were (R2= 0.71 and 0.55). I hope this research can provide decision of managers to helping sustainable management for arid and semi-arid regions in Iran.

  14. NEEM: UNUSUALLY VERSATILE PLANT GENUS AZADIRACHTA WITH MANY USEFUL AND SO FAR INSUFFICIENTLY EXPLOITED PROPERTIES FOR AGRICULTURE, MEDICINE, AND INDUSTRY.

    Science.gov (United States)

    Hummel, H E; Langner, S S; Leithold, G; Schmutterer, H

    2014-01-01

    Neem plants (Rutales: Meliaceae) are well known for their multitude of human benefits in various fields. Specifically well investigated are the Indian neem tree Azadirachta indica A. Juss., the Thai neem A. siamensis Val., the originally Malaysian/Philippinean neem A. excelsa (Jack) and, as a close relative, the Persian lilac, Melia azedarach. The major and most active natural products are azadirachtin, salannin, nimbin and marrangin from Azadirachta species, and azadirachtin analogues like meliantriol from Melia species. Neem fruits, leaves, bark, and roots have specific virtues. They have been traditionally exploited for a considerable part of human history and are documented in Sanskrit texts. Due to human activity in trade and travel both at land and sea, the plant species has been distributed around the globe and is cultivated in many tropical, and subtropical regions. A multitude of natural products of neem have been isolated, chemically characterized or identified, and investigated for their properties in the management of insects, Acarina, Crustacea, nematodes, bacteria, fungi, viruses and soil fertility (for reviews see Kraus, 2002; Schmutterer, 2002A; Rembold, 2002; Koul, 2004; Schmutterer and Huber, 2005; Kleeberg and Strang, 2009; Hummel et al., 2008, 2011, 2012). Neem products are virtually nontoxic, compatible with beneficial insects, pollinators and bees. They are environmentally benign, sustainable, renewable, and of a price affordable for developed countries. In conclusion, neem is a prime example of a natural resource with many beneficial applications in agriculture, human and veterinary medicine. So far, its use is practically free of resistance problems which are frustratingly prevalent in many areas of synthetic insecticide and drug development. Investigating more neem applications will increase future human welfare and health while being of general ecological benefit to the planet.

  15. Seasonal patterns of leaf gas exchange and water relations in dry rain forest trees of contrasting leaf phenology.

    Science.gov (United States)

    Choat, Brendan; Ball, Marilyn C; Luly, Jon G; Donnelly, Christine F; Holtum, Joseph A M

    2006-05-01

    Diurnal and seasonal patterns of leaf gas exchange and water relations were examined in tree species of contrasting leaf phenology growing in a seasonally dry tropical rain forest in north-eastern Australia. Two drought-deciduous species, Brachychiton australis (Schott and Endl.) A. Terracc. and Cochlospermum gillivraei Benth., and two evergreen species, Alphitonia excelsa (Fenzal) Benth. and Austromyrtus bidwillii (Benth.) Burret. were studied. The deciduous species had higher specific leaf areas and maximum photosynthetic rates per leaf dry mass in the wet season than the evergreens. During the transition from wet season to dry season, total canopy area was reduced by 70-90% in the deciduous species and stomatal conductance (g(s)) and assimilation rate (A) were markedly lower in the remaining leaves. Deciduous species maintained daytime leaf water potentials (Psi(L)) at close to or above wet season values by a combination of stomatal regulation and reduction in leaf area. Thus, the timing of leaf drop in deciduous species was not associated with large negative values of daytime Psi(L) (greater than -1.6 MPa) or predawn Psi(L) (greater than -1.0 MPa). The deciduous species appeared sensitive to small perturbations in soil and leaf water status that signalled the onset of drought. The evergreen species were less sensitive to the onset of drought and g(s) values were not significantly lower during the transitional period. In the dry season, the evergreen species maintained their canopies despite increasing water-stress; however, unlike Eucalyptus species from northern Australian savannas, A and g(s) were significantly lower than wet season values.

  16. Short Communication. Comparing flammability traits among fire-stricken (low elevation and non fire-stricken (high elevation conifer forest species of Europe: A test of the Mutch hypothesis

    Directory of Open Access Journals (Sweden)

    A. P. Dimitrakopoulos

    2013-04-01

    Full Text Available Aim of study. The flammability of the main coniferous forest species of Europe, divided into two groups according to their fire regime and altitudinal distribution, was tested in an effort to detect species-specific differences that may have an influence on community-wide fire regimes.Area of study. Conifer species comprising low- and high-elevation forests in Europe.Materials and Methods. The following conifer species were tested: low elevation; Pinus halepensis (Aleppo pine, Pinus brutia (Turkish pine, Pinus pinaster (maritime pine, Pinus pinea (stone pine and Cupressus sempervirens (cypress, high elevation (i.e., above 600 m a.s.l.; Pinus sylvestris (Scots pine, Abies alba (white fir, Picea excelsa (Norway spruce, Abies borissii regis (Macedonian fir and Pinus nigra (black pine. Flammability assessment (time-to-ignition and ignition temperature was conducted by an innovative ignition apparatus, heat content was measured with an IKA Adiabatic Bomb Calorimeter and ash content by heating 5 g of plant material in a muffle furnace at 650ºC for 1 h. Differences among species was statistically analysed by Duncan’s multiple comparison test.Main results. The results did not distinguish separate groups among traits between fire- and non-fire-stricken communities at the individual species level.Research highlights. Differences in fire regimes among low and high elevation conifer forests could be attributed either to differences in flammability of the plant communities as a whole (i.e., fuelbed or canopy properties vs. individual fuel properties or to other factors (climatic or anthropogenic.Key words: flammability; ignitability; heat content; ash content; conifer species; Mutch hypothesis.

  17. Leishmanicidal activity of the crude extract, fractions and major piperidine alkaloids from the flowers of Senna spectabilis.

    Science.gov (United States)

    de Albuquerque Melo, Gabriela Muniz; Silva, Marcela Campelo Rodrigues; Guimarães, Thaís Pereira; Pinheiro, Kátia Mantovani; da Matta, Carolina Barbosa Brito; de Queiroz, Aline Cavalcanti; Pivatto, Marcos; Bolzani, Vanderlan da Silva; Alexandre-Moreira, Magna Suzana; Viegas, Claudio

    2014-02-15

    Senna spectabilis (sin. Cassia excelsa, C. spectabilis) is an endemic tree of South America and Africa, very common in Brazil, where it is known as "canafistula-de-besouro" and "cassia-do-nordeste". In folk medicine, this plant is indicated for the treatment of constipation, insomnia, anxiety, epilepsy, malaria, dysentery and headache. Phytopharmacological studies have also confirmed anticonvulsive, sedative, anti-malarial, antimicrobial and cytotoxic properties of many parts of S. spectabilis. In this communication, we present a comparative study of the leishmanicidal activity of the crude ethanolic extract, its fractions and also the two major alkaloidal metabolites (-)-cassine/(-)-spectaline, trying to establish a relationship between the presence of piperidine alkaloidal constituents and leishmanicidal activity. The growth inhibitory effect of promastigote forms of Leishmania major was determined for the crude extract, fractions of the flowers of S. spectabilis and a mixture of (-)-cassine/(-)-spectaline in comparison to pentamidine used as standard drug. The cytotoxic effects were assessed on macrophage strain J774 by lactate dehydrogenase assay. Fractions dichloromethane (FL-DCM) and n-butanol (FL-Bu) and a mixture of (-)-cassine/(-)-spectaline (∼7:3) exhibited significant activity against the parasite Leishmania major (IC50 values of 0.6±0.1 μg/ml, 1.6±0.9 μg/ml and 24.9±1.4 μg/ml, respectively), without toxic effects on murine macrophages. Due to the promising results elicited, further studies in vivo need to be performed to confirm the therapeutic potential of Senna spectabilis. Copyright © 2013 Elsevier GmbH. All rights reserved.

  18. Climatically sensitive tree-ring chronologies from Crimea, Ukraine

    Science.gov (United States)

    Solomina, O.; Davi, N.; D Arrigo, R.

    2003-04-01

    Several tree species in Crimea can reach ages of 1000 years or more (Crimea..., 1999), including Taxus baccata L., Arbutus andrachne L., Quercus pubescens Willd, Quercus petraea (Mattuschka) Liebl., Quercus robur L., Juniperus excelsa M.B., and Pistacia mutica Fisch.et Mey. In September 2002, we collected samples from several long-lived tree sites described in the literature (Vulf, 1948, Ivanenko, 1951, Ena, 1983, Podgorniy, 1990), located in the mountains of Central Crimea (Sokolinoye, Chufut-Kale, Chelter) and on the coast of the Black Sea (Ai-Todor, Kharaks, Ai-Petri). The trees sampled generally had 300-350 rings. At Ai-Todor, most oaks, junipers, and pistachio showed decay. However, enough samples of oak, juniper and pine were collected to build three chronologies with good replication over the last 350 years. Long meteorological records (for Sevastopol since 1821, Ai-Petri and Yalta since the 1880's) as well as detailed historical data on extreme climatic events since 1687 (summarized by Borisov 1956) are available for this area and can be used to calibrate and verify the tree growth/climate models. Resulting dendroclimatic reconstructions will be the first from this region. The tree-ring time-series may also be used for archaeological dating of historical wood from several medieval fortresses, towns and palaces. In turn, the archaeological wood could be used to extend the tree-ring time series. Stalactites and stalagmites (Dubliansky, 1977) found in numerous caves, as well as 4000-years old laminated lake sediments (Shostakovich, 1934) are also potentially important sources of paleoclimatic information in the area.

  19. Comparative phylogeography in rainforest trees from Lower Guinea, Africa.

    Directory of Open Access Journals (Sweden)

    Myriam Heuertz

    Full Text Available Comparative phylogeography is an effective approach to assess the evolutionary history of biological communities. We used comparative phylogeography in fourteen tree taxa from Lower Guinea (Atlantic Equatorial Africa to test for congruence with two simple evolutionary scenarios based on physio-climatic features 1 the W-E environmental gradient and 2 the N-S seasonal inversion, which determine climatic and seasonality differences in the region. We sequenced the trnC-ycf6 plastid DNA region using a dual sampling strategy: fourteen taxa with small sample sizes (dataset 1, mean n = 16/taxon, to assess whether a strong general pattern of allele endemism and genetic differentiation emerged; and four taxonomically well-studied species with larger sample sizes (dataset 2, mean n = 109/species to detect the presence of particular shared phylogeographic patterns. When grouping the samples into two alternative sets of two populations, W and E, vs. N and S, neither dataset exhibited a strong pattern of allelic endemism, suggesting that none of the considered regions consistently harboured older populations. Differentiation in dataset 1 was similarly strong between W and E as between N and S, with 3-5 significant F ST tests out of 14 tests in each scenario. Coalescent simulations indicated that, given the power of the data, this result probably reflects idiosyncratic histories of the taxa, or a weak common differentiation pattern (possibly with population substructure undetectable across taxa in dataset 1. Dataset 2 identified a common genetic break separating the northern and southern populations of Greenwayodendron suaveolens subsp. suaveolens var. suaveolens, Milicia excelsa, Symphonia globulifera and Trichoscypha acuminata in Lower Guinea, in agreement with differentiation across the N-S seasonal inversion. Our work suggests that currently recognized tree taxa or suspected species complexes can contain strongly differentiated genetic lineages

  20. Ecosystem consequences of tree monodominance for nitrogen cycling in lowland tropical forest.

    Science.gov (United States)

    Brookshire, E N Jack; Thomas, Steven A

    2013-01-01

    Understanding how plant functional traits shape nutrient limitation and cycling on land is a major challenge in ecology. This is especially true for lowland forest ecosystems of the tropics which can be taxonomically and functionally diverse and rich in bioavailable nitrogen (N). In many tropical regions, however, diverse forests occur side-by-side with monodominant forest (one species >60% of canopy); the long-term biogeochemical consequences of tree monodominance are unclear. Particularly uncertain is whether the monodominant plant-soil system modifies nutrient balance at the ecosystem level. Here, we use chemical and stable isotope techniques to examine N cycling in old-growth Mora excelsa and diverse watershed rainforests on the island of Trinidad. Across 26 small watershed forests and 4 years, we show that Mora monodominance reduces bioavailable nitrate in the plant-soil system to exceedingly low levels which, in turn, results in small hydrologic and gaseous N losses at the watershed-level relative to adjacent N-rich diverse forests. Bioavailable N in soils and streams remained low and remarkably stable through time in Mora forests; N levels in diverse forests, on the other hand, showed high sensitivity to seasonal and inter-annual rainfall variation. Total mineral N losses from diverse forests exceeded inputs from atmospheric deposition, consistent with N saturation, while losses from Mora forests did not, suggesting N limitation. Our measures suggest that this difference cannot be explained by environmental factors but instead by low internal production and efficient retention of bioavailable N in the Mora plant-soil system. These results demonstrate ecosystem-level consequences of a tree species on the N cycle opposite to cases where trees enhance ecosystem N supply via N2 fixation and suggest that, over time, Mora monodominance may generate progressive N draw-down in the plant-soil system.

  1. Ecosystem consequences of tree monodominance for nitrogen cycling in lowland tropical forest.

    Directory of Open Access Journals (Sweden)

    E N Jack Brookshire

    Full Text Available Understanding how plant functional traits shape nutrient limitation and cycling on land is a major challenge in ecology. This is especially true for lowland forest ecosystems of the tropics which can be taxonomically and functionally diverse and rich in bioavailable nitrogen (N. In many tropical regions, however, diverse forests occur side-by-side with monodominant forest (one species >60% of canopy; the long-term biogeochemical consequences of tree monodominance are unclear. Particularly uncertain is whether the monodominant plant-soil system modifies nutrient balance at the ecosystem level. Here, we use chemical and stable isotope techniques to examine N cycling in old-growth Mora excelsa and diverse watershed rainforests on the island of Trinidad. Across 26 small watershed forests and 4 years, we show that Mora monodominance reduces bioavailable nitrate in the plant-soil system to exceedingly low levels which, in turn, results in small hydrologic and gaseous N losses at the watershed-level relative to adjacent N-rich diverse forests. Bioavailable N in soils and streams remained low and remarkably stable through time in Mora forests; N levels in diverse forests, on the other hand, showed high sensitivity to seasonal and inter-annual rainfall variation. Total mineral N losses from diverse forests exceeded inputs from atmospheric deposition, consistent with N saturation, while losses from Mora forests did not, suggesting N limitation. Our measures suggest that this difference cannot be explained by environmental factors but instead by low internal production and efficient retention of bioavailable N in the Mora plant-soil system. These results demonstrate ecosystem-level consequences of a tree species on the N cycle opposite to cases where trees enhance ecosystem N supply via N2 fixation and suggest that, over time, Mora monodominance may generate progressive N draw-down in the plant-soil system.

  2. Socio-ecological costs of Amazon nut and timber production at community household forests in the Bolivian Amazon.

    Science.gov (United States)

    Soriano, Marlene; Mohren, Frits; Ascarrunz, Nataly; Dressler, Wolfram; Peña-Claros, Marielos

    2017-01-01

    The Bolivian Amazon holds a complex configuration of people and forested landscapes in which communities hold secure tenure rights over a rich ecosystem offering a range of livelihood income opportunities. A large share of this income is derived from Amazon nut (Bertholletia excelsa). Many communities also have long-standing experience with community timber management plans. However, livelihood needs and desires for better living conditions may continue to place these resources under considerable stress as income needs and opportunities intensify and diversify. We aim to identify the socioeconomic and biophysical factors determining the income from forests, husbandry, off-farm and two keystone forest products (i.e., Amazon nut and timber) in the Bolivian Amazon region. We used structural equation modelling tools to account for the complex inter-relationships between socioeconomic and biophysical factors in predicting each source of income. The potential exists to increase incomes from existing livelihood activities in ways that reduce dependency upon forest resources. For example, changes in off-farm income sources can act to increase or decrease forest incomes. Market accessibility, social, financial, and natural and physical assets determined the amount of income community households could derive from Amazon nut and timber. Factors related to community households' local ecological knowledge, such as the number of non-timber forest products harvested and the number of management practices applied to enhance Amazon nut production, defined the amount of income these households could derive from Amazon nut and timber, respectively. The (inter) relationships found among socioeconomic and biophysical factors over income shed light on ways to improve forest-dependent livelihoods in the Bolivian Amazon. We believe that our analysis could be applicable to other contexts throughout the tropics as well.

  3. NTFP harvesters as citizen scientists: Validating traditional and crowdsourced knowledge on seed production of Brazil nut trees in the Peruvian Amazon.

    Science.gov (United States)

    Thomas, Evert; Valdivia, Jheyson; Alcázar Caicedo, Carolina; Quaedvlieg, Julia; Wadt, Lucia Helena O; Corvera, Ronald

    2017-01-01

    Understanding the factors that underlie the production of non-timber forest products (NTFPs), as well as regularly monitoring production levels, are key to allow sustainability assessments of NTFP extractive economies. Brazil nut (Bertholletia excelsa, Lecythidaceae) seed harvesting from natural forests is one of the cornerstone NTFP economies in Amazonia. In the Peruvian Amazon it is organized in a concession system. Drawing on seed production estimates of >135,000 individual Brazil nut trees from >400 concessions and ethno-ecological interviews with >80 concession holders, here we aimed to (i) assess the accuracy of seed production estimates by Brazil nut seed harvesters, and (ii) validate their traditional ecological knowledge (TEK) about the variables that influence Brazil nut production. We compared productivity estimates with actual field measurements carried out in the study area and found a positive correlation between them. Furthermore, we compared the relationships between seed production and a number of phenotypic, phytosanitary and environmental variables described in literature with those obtained for the seed production estimates and found high consistency between them, justifying the use of the dataset for validating TEK and innovative hypothesis testing. As expected, nearly all TEK on Brazil nut productivity was corroborated by our data. This is reassuring as Brazil nut concession holders, and NTFP harvesters at large, rely on their knowledge to guide the management of the trees upon which their extractive economies are based. Our findings suggest that productivity estimates of Brazil nut trees and possibly other NTFP-producing species could replace or complement actual measurements, which are very expensive and labour intensive, at least in areas where harvesters have a tradition of collecting NTFPs from the same trees over multiple years or decades. Productivity estimates might even be sourced from harvesters through registers on an annual basis

  4. NTFP harvesters as citizen scientists: Validating traditional and crowdsourced knowledge on seed production of Brazil nut trees in the Peruvian Amazon.

    Directory of Open Access Journals (Sweden)

    Evert Thomas

    Full Text Available Understanding the factors that underlie the production of non-timber forest products (NTFPs, as well as regularly monitoring production levels, are key to allow sustainability assessments of NTFP extractive economies. Brazil nut (Bertholletia excelsa, Lecythidaceae seed harvesting from natural forests is one of the cornerstone NTFP economies in Amazonia. In the Peruvian Amazon it is organized in a concession system. Drawing on seed production estimates of >135,000 individual Brazil nut trees from >400 concessions and ethno-ecological interviews with >80 concession holders, here we aimed to (i assess the accuracy of seed production estimates by Brazil nut seed harvesters, and (ii validate their traditional ecological knowledge (TEK about the variables that influence Brazil nut production. We compared productivity estimates with actual field measurements carried out in the study area and found a positive correlation between them. Furthermore, we compared the relationships between seed production and a number of phenotypic, phytosanitary and environmental variables described in literature with those obtained for the seed production estimates and found high consistency between them, justifying the use of the dataset for validating TEK and innovative hypothesis testing. As expected, nearly all TEK on Brazil nut productivity was corroborated by our data. This is reassuring as Brazil nut concession holders, and NTFP harvesters at large, rely on their knowledge to guide the management of the trees upon which their extractive economies are based. Our findings suggest that productivity estimates of Brazil nut trees and possibly other NTFP-producing species could replace or complement actual measurements, which are very expensive and labour intensive, at least in areas where harvesters have a tradition of collecting NTFPs from the same trees over multiple years or decades. Productivity estimates might even be sourced from harvesters through registers on an

  5. Indicator species of essential forest tree species in the Burdur district.

    Science.gov (United States)

    Negiz, Mehmet Güvenç; Eser, Yunus; Kuzugüdenll, Emre; Izkan, Kürşad

    2015-01-01

    The forests of Burdur district for long have been subjected to over grazing and individual selection. As a result of this, majority of the forest areas in the district were degraded. In the district, afforestation efforts included majority of forestry implementations. It is well known that selecting suitable species plays an important role for achieving afforestation efforts. In this context, knowing the indicator species among the target species would be used in afforestation efforts, studies on the interrelationships between environmental factors and target species distribution is vital for selecting suitable species for a given area. In this study, Anatolian Black pine (Pinus nigra), Red pine (Pinus brutia), Crimean juniper (Juniperus excelsa) and Taurus cedar (Cedrus libani), essential tree species, were considered as target species. The data taken from 100 sample plots in Burdur district was used. Interspecific correlation analysis was performed to determine the positive and negative indicator species among each of the target species. As a result of ICA, 2 positive (Berberis crataegina, Juniperus oxycedrus), 2 negative (Phillyrea latifolia, Quercus coccifera) for Crimean Juniper, I positive (Juniperus oxycedrus), 3 negative (Onopordium acanthium, Fraxinus ornus, Phillyrea latifolia) for Anatolian black pine, 3 positive (Paliurus spina-christi, Quercus coccifer, Crataegus orientalis), 2 negative (Berberis crataegina, Astragalus nanus) for Red pine and 3 positive (Berberis crataegina, Rhamnus oleoides, Astragalus prusianus) 2 negative (Paliurus spina-christi, Quercus cerris) for Taurus cedarwere defined as indicator plant species. In this way, practical information was obtained for selecting the most suitable species, among the target species, for afforestation efforts in Burdur district.

  6. Species choice, provenance and species trials among native Brazilian species

    Energy Technology Data Exchange (ETDEWEB)

    Drumond, M A

    1982-01-01

    Six papers from the conference are presented. Drumond, M.A., Potential of species native to the semi-arid tropics, 766-781, (Refs. 18), reports on Anadenanthera macrocarpa, Mimosa species, Schinopsis brasiliensis, Spondias tuberosa, Ziziphus joazeiro, Cnidoscolus phyllacanthus, Bursera leptophleos (leptophloeos), Tabebuia impetiginosa, Astronium urundeuva, and Mimosa caesalpinia. Monteiro, R.F.R., Speltz, R.M., Gurgel, J.T. do A.; Silvicultural performance of 24 provenances of Araucaria angustifolia in Parana, 814-824, (Refs. 8). Pires, C.L. da S., Kalil Filho, A.N., Rosa, P.R.F. da, Parente, P.R., Zanatto, A.C.S.; Provenance trials of Cordia alliodora in the State of Sao Paulo, 988-995, (Refs. 9). Nogueira, J.C.B., Siqueira, A.C.M.F., Garrido, M.A.O., Gurgel Garrido, L.M. do A., Rosa, P.R.F., Moraes, J.L. de, Zandarin, M.A., Gurgel Filho, O.A., Trials of some native species in various regions of the State of Sao Paulo, 1051-1063, (Refs. 9) describes Centrolobium tomentosum, Peltophorum dubium, Tabebuia vellosoi, Cariniana legalis, and Balfourodendron riedelianum. Batista, M.P., Borges, J.F., Franco, M.A.B.; Early growth of a native species in comparison with exotics in northeastern Para, Brazil, 1105-1110, (Refs. 3). Jacaranda copaia is compared with Gmelina arborea, Pinus caribaea various hondurensis, Eucalyptus deglupta, and E. urophylla. Lima, P.C.F., Souza, S.M. de, Drumond, M.A.; Trials of native forest species at Petrolina, Pernambuco, 1139-1148, (Refs. 8), deals with Anadenanthera macrocarpa, Piptadenia obliqua, Pithecellobium foliolosum, Astronium urundeuva, Schinopsis brasiliensis, Cassia excelsa, Caesalpinia pyramidalis, Parkia platycephala, Pseudobombax simplicifolium, Tabebuia impetiginosa, Caesalpinia ferrea, and Aspidosperma pyrifolium. 18 references.

  7. Short communication.Wood identification based on their common name and their transversal surface anatomy.Application to the batch from the expedition of Ruiz and Pavon

    Energy Technology Data Exchange (ETDEWEB)

    Dimitrakopoulos, A. P.; Mitsopoulos, I. D.; Kaliva, A.

    2013-05-01

    Aim of study: The flammability of the main coniferous forest species of Europe, divided into two groups according to their fire regime and altitudinal distribution, was tested in an effort to detect species-specific differences that may have an influence on community-wide fire regimes. Area of study: Conifer species comprising low- and high-elevation forests in Europe. Materials and methods: The following conifer species were tested: low elevation; Pinus halepensis (Aleppo pine), Pinus brutia (Turkish pine), Pinus pinaster (maritime pine), Pinus pinea (stone pine) and Cupressus sempervirens (cypress), high elevation (i.e., above 600 m a.s.l.); Pinus sylvestris (Scots pine), Abies alba (white fir), Picea excelsa (Norway spruce), Abies borissii regis (Macedonian fir) and Pinus nigra (black pine). Flammability assessment (time-to-ignition and ignition temperature) was conducted by an innovative ignition apparatus, heat content was measured with an IKA Adiabatic Bomb Calorimeter and ash content by heating 5 g of plant material in a muffle furnace at 650 degree centigrade for 1 h. Differences among species was statistically analysed by Duncan's multiple comparison test. Main results: The results did not distinguish separate groups among the tested flammability traits between fire- and non-fire-stricken communities at the individual species level. Research highlights: Differences in fire regimes among low and high elevation conifer forests could be attributed either to differences in flammability of the plant communities as a whole (i.e., fuelbed or canopy properties vs. individual fuel properties) or to other factors (climatic or anthropogenic). (Author) 22 refs.

  8. The profile of Brazilian agriculture as source of raw material to obtain organic cosmetics

    Directory of Open Access Journals (Sweden)

    Neila de Paula Pereira

    2017-05-01

    Full Text Available With one of the most notable floras in the world for sustainable research, the Brazilian Amazon region currently counts on financial incentives from the Brazilian Government for private national and foreign businesses. The ongoing implantation of a Biocosmetics Research and Development Network (REDEBIO aims to stimulate research involving natural resources from the Brazilian states that make up the zone defined as “Amazônia Legal”. The objective of this region, still under development in Brazil, is principally to aggregate value to products manufactured in small local industries through the use of sustainable technology currently being established. Certain certified raw materials already included in the country’s sustainability program, have also begun to be cultivated according to the requirements of organic cultivation (Neves, 2009. The majority are species of Amazonian vegetation: Euterpe oleracea (Açai, Orbignya martiana (Babaçu, Theobroma grandi-florum (Cupuaçu, Carapas guianensis (Andiroba, Pentaclethra macroloba (Pracaxi, Copaifera landesdorffi (Copaiba, Platonia insignis (Bacuri, Theobroma cacao (Cacao, Virola surinamensis (Ucuuba and Bertholletia excelsa (Brazil nut. These generate phytopreparations, such as oils, extracts, and dyes that are widely used in the manufacture of Brazilian organic cosmetics with scientifically proven topical and capillary benefits. In the final balance, Brazilian organic cosmetics should continue to gain force over the next few years, especially with the regulation of the organic cosmetics market that is being drafted by the Brazilian Ministry of Agriculture. Moreover, lines of ecologically aware products that provide quality of life for both for rural and metropolitan communities show a tendency to occupy greater space in the market.

  9. Determinação de parâmetros físico-químicos e da origem botânica de méis indicados monoflorais do sudeste do Brasil Physico-chemical parameters and botanical origin of indicated monofloral honeys from the southeast of Brazil

    Directory of Open Access Journals (Sweden)

    Monika O. Barth

    2005-06-01

    Full Text Available O presente trabalho analisou a composição físico-química e origem botânica de 31 amostras de mel indicadas como sendo de origem monofloral, comercializadas e produzidas na região Sudeste do Brasil, a fim de verificar parâmetros de qualidade. As análises físico-químicas compreenderam o teste de Fiehe, a reação de lugol, a determinação do teor de umidade, do pH, de açúcares redutores, de cinzas e do índice de diastase. Todas as amostras apresentaram-se dentro do limite previsto pela legislação brasileira para o teor de umidade. Dez amostras apresentaram teor de açúcares redutores inferior ao previsto na legislação, três tinham número de diastase (unidades Schade/Gothe inferior a 8, uma apresentou pH abaixo do padrão e outra apresentou teor de cinzas superior ao previsto. A análise polínica mostrou que cerca de 57% das amostras poderiam ser classificadas como monoflorais, correspondendo nove amostras a mel de eucalipto (Eucalyptus, Myrtaceae, duas a mel de aroeira (Schinus, Anacardiaceae, duas a mel de assa-peixe (Vernonia, Asteraceae, duas a mel de laranjeira (Citrus, Rutaceae, uma a mel de cambará (Gochnatia, uma a mel de capixingui (Croton, Euphorbiaceae. As demais amostras eram bi- ou heteroflorais. A influência de uma determinada espécie botânica nas variáveis físico-químicas analisadas não foi significativa (p>0,05 segundo a análise de regressão.The physico-chemical properties and the botanical origin of 31 commercial honey samples from Southeast Brazil, indicated as monofloral honeys, were analyzed in order to verify quality parameters. The physico-chemical analyses comprised the test of Fiehe, the lugol reaction, the determination of humidity, pH, reduced sugars, ashes and diastase. In accordance with Brazilian legislation all samples showed adequate water content. The level of reducing sugars from 10 samples was below the Brazilian quality parameters for honey, three presented a Schade/Gothe number of

  10. Chromosome studies on Brazilian cerrado plants

    Directory of Open Access Journals (Sweden)

    Eliana Regina Forni-Martins

    2000-12-01

    Full Text Available Cerrado is the Brazilian name for the neotropical savanna, which occurs mainly in Brazilian Central Plateau, composed of herbaceous-subshrubby and shrubby-arboreal floras, both of which are heliophilous, highly diverse and regionally differentiated. Considering species distribution and chromosome numbers, some authors have proposed that the herbaceous-subshrubby flora of the neotropical savanna is quite old, while the shrubby-arboreal flora is derived from forests, a hypothesis that implies higher chromosome numbers in the savanna than in the forest. If, however, chromosome numbers are similar in the cerrado and in forests, both could be similarly old, indicating that bi-directional flow of flora occurred in the past. This paper presents data on chromosome numbers and microsporogenesis for 20 species in 13 families collected in the States of São Paulo, Goiás and Minas Gerais, providing previously unpublished data for Myrcia (Myrtaceae, Luxemburgia (Ochnaceae and Hortia (Rutaceae. Meiosis proved to be normal, indicating regularity in the sexual reproductive process. Chromosome numbers varied from 2n = 18 (Allamanda angustifolia: Apocynaceae to 2n = ca. 104 (Ouratea spectabilis: Ochnaceae, being low (20 Cerrado é a palavra que, no Brasil, designa a savana neotropical, com área nuclear no Planalto Central, constituída de uma flora herbáceo-subarbustiva e outra arbustivo-arbórea, ambas heliófilas, altamente diversificadas e regionalmente diferenciadas. Considerando a distribuição de espécies e de números cromossômicos, alguns autores propuseram que a flora herbáceo-subarbustiva da savanna neotropical seria bastante antiga, enquanto a flora arbustivo-arbórea seria derivada das florestas Atlântica e Amazônica, uma hipótese que implica na ocorrência de números cromossômicos mais altos no cerrado que nas florestas. Porém, se os números cromossômicos forem similares no cerrado e nas florestas, ambos os tipos de formação poderiam

  11. The status of conservation of urban forests in eastern Amazonia

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    DD Amaral

    Full Text Available This study aims to identify the remnant tree flora in six forest fragments in the metropolitan area of Belém and to analyze these fragments in terms of biological conservation, species richness and diversity in the local urban landscape. The fragments and their respective sampling areas were as follows: Amafrutas reserve (15 ha, Trambioca Is. reserve (2 ha, Bosque Rodrigues Alves city park (15 ha, Combu Is. reserve (10 ha, Gunma Park reserve (10 ha and Mocambo reserve (5 ha. Inventories were built from lineal plots of 250 m² and included trees with DBH equal to or greater than 10 cm at a height of 1.3 m above ground. Sixty-nine families and 759 species, of which eight were officially listed as endangered (Brazilian National Flora: Ministry of Environment, Normative Instruction of September, 2008; Pará State Flora: Decree Nº. 802 of February 2008 were recorded. These endangered species are: Aspidosperma desmanthum Benth. ex Müll. Arg. (Apocynaceae, Cedrela odorata L. (Meliaceae, Eschweilera piresii S.A Mori (Lecythidaceae, Euxylophora paraensis Huber (Rutaceae, Hymenolobium excelsum Ducke (Leguminosae, Manilkara huberi (Ducke Chevalier (Sapotaceae, Tabebuia impetiginosa (Mart. ex DC. Standl. (Bignoniaceae, Mezilaurus itauba (Meisn. Taub. ex Mez (Lauraceae and Qualea coerulea Aubl. (Vochysiaceae. Emergency actions such as implementing management plans for already existing Conservation Units, the creation of new such units in areas of primary forest fragments (as in the case of the Amafrutas reserve, as well as the intensification of actions of surveillance and monitoring, should be undertaken by Federal, State, and Municipal environmental agencies so as to ensure the conservation of these last primary forest remnants in the metropolitan area of Belém.

  12. A draft of the genome and four transcriptomes of a medicinal and pesticidal angiosperm Azadirachta indica

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    Krishnan Neeraja M

    2012-09-01

    Full Text Available Abstract Background The Azadirachta indica (neem tree is a source of a wide number of natural products, including the potent biopesticide azadirachtin. In spite of its widespread applications in agriculture and medicine, the molecular aspects of the biosynthesis of neem terpenoids remain largely unexplored. The current report describes the draft genome and four transcriptomes of A. indica and attempts to contextualise the sequence information in terms of its molecular phylogeny, transcript expression and terpenoid biosynthesis pathways. A. indica is the first member of the family Meliaceae to be sequenced using next generation sequencing approach. Results The genome and transcriptomes of A. indica were sequenced using multiple sequencing platforms and libraries. The A. indica genome is AT-rich, bears few repetitive DNA elements and comprises about 20,000 genes. The molecular phylogenetic analyses grouped A. indica together with Citrus sinensis from the Rutaceae family validating its conventional taxonomic classification. Comparative transcript expression analysis showed either exclusive or enhanced expression of known genes involved in neem terpenoid biosynthesis pathways compared to other sequenced angiosperms. Genome and transcriptome analyses in A. indica led to the identification of repeat elements, nucleotide composition and expression profiles of genes in various organs. Conclusions This study on A. indica genome and transcriptomes will provide a model for characterization of metabolic pathways involved in synthesis of bioactive compounds, comparative evolutionary studies among various Meliaceae family members and help annotate their genomes. A better understanding of molecular pathways involved in the azadirachtin synthesis in A. indica will pave ways for bulk production of environment friendly biopesticides.

  13. Evaluation of Antibacterial Activities of Citrus limon, Citrus reticulata, and Citrus grandis Against Pathogenic Bacteria

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    Sholeh Saeb

    2016-11-01

    Full Text Available Background: Microorganisms resistant to most antibiotics are rapidly spreading, and there is an urgent and continuous need for novel antimicrobial compounds. The genus Citrus belongs to the family Rutaceae has many biologically active secondary metabolites. Objectives: The purpose of this study was to evaluate antimicrobial activity of essential oil and extract of Lemon (Citrus limon, Mandarin (Citrus reticulata and Pummelo (Citrus grandis against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella typhi. Materials and Methods: The fresh Citrus leaves were shade-dried and powdered. Antimicrobial metabolites were extracted from them by 80% methanol for extract and using a Clevenger-type apparatus for essential oil. Eight different concentrations of the each leaf extract and essential oil were prepared. The antimicrobial susceptibility assay of Citrus leaves metabolites were subjected against four bacterial strains by agar disc diffusion and E-test method. Results: In this study, minimum inhibitory concentrations (MIC of different Citrus leaf extracts were determined against all four food-borne pathogens. The C. grandis leaf essential oil had potent antimicrobial activity against all four pathogens, and the C. limon leaf essential oil was effective on Gram-positive bacteria. S. typhi was resistant against two leaves essential oils. Conclusions: The results showed that there was no antimicrobial activity effect in all extracts on tested bacteria. In this study, the antibacterial effect of essential oil of Citrus leaves on four strains of pathogenic microorganisms was confirmed. The C. grandis leaf essential oil had the most powerful antimicrobial properties, suggesting its potential application as natural preservative in foods or an effective medicine against different pathogenic microbes. Key words: Antibacterial activity, E-test, Citr

  14. Medicinal plants used for hypertension treatment by folk healers in Songkhla province, Thailand.

    Science.gov (United States)

    Neamsuvan, Oratai; Komonhiran, Panadda; Boonming, Kamonvadee

    2018-03-25

    Hypertension is the most dominant risk factor for the development of cardiovascular, kidney, and eye diseases. In Thailand, illness and hospitalisation in the modern public health system due to high blood pressure is increasing. However, some Thai people have turned their attention to the use of herbal medicines for healthcare. Therefore, this study aimed (1) to study the folk knowledge of hypertension treatment and (2) to study plant utilisation in the treatment of high blood pressure by Songkhla folk healers. Field surveys and semi-structured interviews about theories of disease, principles of healing, and herbal usage (plant species, parts used, preparation, and application methods) were gathered. The data were analysed by descriptive statistics. The literatures regarding medicinal plants used in any traditional medicine, antihypertension activity, and toxicity was reviewed. Most healers believed that hypertension was caused by the disorder of fire and wind elements in the body. The medicinal plants containing hot and mild tastes, which had the potential for treating problems in the wind element, were applied. A total of 62 species were used for hypertension treatment. Most plants were in the Asteraceae, Piperaceae, Rutaceae, or Zingiberaceae family (4 species each). Herbal medicines were preferred to be prepared by boiling (78%) and consumed by drinking 1 teacup before 3 meals each day (26%). Piper retrofractum and Cleome viscosa had the greatest Frequency of Citation (FC = 6, n = 14). Thirty-seven species have been reported for use in traditional medicine. Twenty-four and 46 species have already been investigated for antihypertension activity and toxicity, respectively. Identifying medicinal plants that have been tested by experienced folk doctors would provide an opportunity for people to choose and consume local herbs that are easy to access in their local area. However, the remaining plants that have not been studied for antihypertension activity and

  15. Medicinal plants used by the people of Nsukka Local Government Area, south-eastern Nigeria for the treatment of malaria: An ethnobotanical survey.

    Science.gov (United States)

    Odoh, Uchenna E; Uzor, Philip F; Eze, Chidimma L; Akunne, Theophine C; Onyegbulam, Chukwuma M; Osadebe, Patience O

    2018-05-23

    Malaria is a serious public health problem especially in sub-Saharan African countries such as Nigeria. The causative parasite is increasingly developing resistance to the existing drugs. There is urgent need for alternative and affordable therapy from medicinal plants which have been used by the indigenous people for many years. This study was conducted to document the medicinal plant species traditionally used by the people of Nsukka Local Government Area in south-eastern Nigeria for the treatment of malaria. A total of 213 respondents, represented by women (59.2%) and men (40.8%), were interviewed using a semi-structured questionnaire. The results were analysed and discussed in the context of previously published information on anti-malarial and phytochemical studies of the identified plants. The survey revealed that 50 plant species belonging to 30 botanical families were used in this region for the treatment of malaria. The most cited families were Apocynaceae (13.3%), Annonaceae (10.0%), Asteraceae (10.0%), Lamiaceae (10.0%), Poaceae (10.0%), Rubiaceae (10.0%) and Rutaceae (10.0%). The most cited plant species were Azadirachta indica (11.3%), Mangifera indica (9.1%), Carica papaya (8.5%), Cymbopogon citratus (8.5%) and Psidium guajava (8.5%). The present findings showed that the people of Nsukka use a large variety of plants for the treatment of malaria. The identified plants are currently undergoing screening for anti-malarial, toxicity and chemical studies in our laboratory. Copyright © 2018 Elsevier B.V. All rights reserved.

  16. Taxonomic research priorities for the conservation of the South African flora

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    Lize von Staden

    2013-04-01

    Full Text Available Taxonomic revisions, monographs and floras are the most important, and often the only source of data for assessing the extinction risk of plants, with recent revisions contributing to more accurate assessments. The recently completed Red List of South African plants involved an overview of the taxonomic literature pertaining to the South African flora, providing an opportunity to identify critical gaps in taxonomic coverage. In this study we identified taxonomic research priorities for effective conservation of South African plants. Priorities were identified at genus level, according to time since last revision, level of endemism, collecting effort, proportion of taxa included in revisions, and specimen identification confidence. Although the results indicate that 62% of the flora has been recently revised, revisionary taxonomic output has declined drastically, particularly in the past 10 years. This decline is a result of a decrease in revisionary productivity per taxonomist and not a result of a decline in the number of working taxonomists. The family Aizoaceae is the top priority for taxonomic research with 55% of taxa in need of revision, followed by Hyacinthaceae with 34% of taxa not yet revised. Ericaceae, Euphorbiaceae, Rutaceae, Malvaceae, Asteraceae and Acanthaceae are also priorities with over 30% of taxa last revised before 1970. We recommend the reinstatement of the Flora of Southern Africa project in an online format in order to centralise South Africa's existing taxonomic information and reinvigorate revisionary taxonomic study. This project will allow South Africa to fulfil its commitments to the Convention on Biodiversity by achieving Target 1 of the Global Strategy for Plant Conservation.

  17. Authentication of ruta graveolens and its adulterant using internal transcribed spacer (its) sequences of nuclear ribosomal DNA

    International Nuclear Information System (INIS)

    Qurainy, F.A.; Khan, S.; Ali, M.A.; Hemaid, M.A.; Ashraf, M.

    2011-01-01

    Ruta graveolens L. (Rutaceae) is commonly known as 'Sudab' which is well known for hippocratic medicine and is commonly used in indigenous health-care system in India. Euphorbia dracunculoides Lam. (Euphorbiaceae) in raw drug trading has almost similar morphology to R. graveolens in dried state, is being sold locally or used clinically as an adulterant of R. graveolens (genuine) at a relatively low price under the same name 'Sudab' which has ultimately reduced the efficacy and quality of this herb. The internal transcribed spacer (ITS) sequence of nuclear ribosomal DNA gene of genuine and adulterant were sequenced and analyzed to assess species admixture in raw drug trading of genuine herbal drug. The BLAST search results of ITS sequence of genuine sample of 'Sudab' i.e., R. graveolens showed 99% similarity to the sequence of R. graveolens, however, E. dracunculoides showed 100% similarity to the species of Euphorbia and did not show any similarity with R. graveolens. The sequence alignment of both species was entirely different to each other. Phylogenetic analysis based on ITS sequence of adulterant sample i.e., E. dracunculoides together with sequences of Euphorbia species available in the GenBank has also clearly showed its nesting within the Euphorbia tree. The generated ITS sequences of both samples in the present study may be referred hereafter as species-specific DNA barcode signature, which can be used in authenticating and validating the exact species identities to discriminate the genuine sample of 'Sudab' from its adulterants if any available to guarantee the quality and purity of this drug in the herbal drug market. (author)

  18. Mapping and Assessment of Ethno-Medicinal Trees in Built Up Areas - University of Port Harcourt, Nigeria

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    Olatunde Sunday Eludoyin

    2015-06-01

    Full Text Available Background and Purpose: Several urban tree species are important in ethno-medicine, especially in the developing tropical regions. Their assessment in urban landscapes is becoming an important issue. The study assessed and mapped the ethno-medicinal trees in the built up area land use type of the University of Port Harcourt, Nigeria, with a view to examining their spatial variation in terms of composition and diversity between the residential and non-residential areas of the University Park. Materials and Methods: The study employed the use of geographic information system (ArcGIS 9.3 for the mapping. Built up area land use was subdivided into residential and non-residential where the ethno-medicinal trees were recorded, identified and enumerated. Global positioning system was used to determine the coordinates of each tree. The species composition and diversity were calculated and a comparison was made between the residential and non- residential land use types. The pattern of spread of the ethno-medicinal trees was determined by the nearest neighbour analysis. Results: A total of 37 ethno-medicinal trees species were found in the study area, while the species composition was 499 in the residential area and 438 in the non-residential area. Azadirachta indica was the highest (233 in composition. Ethno-medicinal tree species in the study area consist of 19 families of which Anacardiaceae, Rutaceae, Moraceae and Combretaceae were the highest. Species diversity was higher in the non-residential land use (2.698 than in the residential land use (2.222. Conclusion: The nearest neighbour analysis reveals that the z-score value was higher in the non-residential area (-23.06 than in the residential area (-0.30, but the pattern of distribution in both areas were clustered. The study recommended periodic monitoring and the assessment of ethno-medicinal trees in the study area for conservation purposes.

  19. Phylogenetic Relationships of Citrus and Its Relatives Based on matK Gene Sequences

    Science.gov (United States)

    Penjor, Tshering; Uehara, Miki; Ide, Manami; Matsumoto, Natsumi; Matsumoto, Ryoji

    2013-01-01

    The genus Citrus includes mandarin, orange, lemon, grapefruit and lime, which have high economic and nutritional value. The family Rutaceae can be divided into 7 subfamilies, including Aurantioideae. The genus Citrus belongs to the subfamily Aurantioideae. In this study, we sequenced the chloroplast matK genes of 135 accessions from 22 genera of Aurantioideae and analyzed them phylogenetically. Our study includes many accessions that have not been examined in other studies. The subfamily Aurantioideae has been classified into 2 tribes, Clauseneae and Citreae, and our current molecular analysis clearly discriminate Citreae from Clauseneae by using only 1 chloroplast DNA sequence. Our study confirms previous observations on the molecular phylogeny of Aurantioideae in many aspects. However, we have provided novel information on these genetic relationships. For example, inconsistent with the previous observation, and consistent with our preliminary study using the chloroplast rbcL genes, our analysis showed that Feroniella oblata is not nested in Citrus species and is closely related with Feronia limonia. Furthermore, we have shown that Murraya paniculata is similar to Merrillia caloxylon and is dissimilar to Murraya koenigii. We found that “true citrus fruit trees” could be divided into 2 subclusters. One subcluster included Citrus, Fortunella, and Poncirus, while the other cluster included Microcitrus and Eremocitrus. Compared to previous studies, our current study is the most extensive phylogenetic study of Citrus species since it includes 93 accessions. The results indicate that Citrus species can be classified into 3 clusters: a citron cluster, a pummelo cluster, and a mandarin cluster. Although most mandarin accessions belonged to the mandarin cluster, we found some exceptions. We also obtained the information on the genetic background of various species of acid citrus grown in Japan. Because the genus Citrus contains many important accessions, we have

  20. Phylogenetic relationships of citrus and its relatives based on matK gene sequences.

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    Tshering Penjor

    Full Text Available The genus Citrus includes mandarin, orange, lemon, grapefruit and lime, which have high economic and nutritional value. The family Rutaceae can be divided into 7 subfamilies, including Aurantioideae. The genus Citrus belongs to the subfamily Aurantioideae. In this study, we sequenced the chloroplast matK genes of 135 accessions from 22 genera of Aurantioideae and analyzed them phylogenetically. Our study includes many accessions that have not been examined in other studies. The subfamily Aurantioideae has been classified into 2 tribes, Clauseneae and Citreae, and our current molecular analysis clearly discriminate Citreae from Clauseneae by using only 1 chloroplast DNA sequence. Our study confirms previous observations on the molecular phylogeny of Aurantioideae in many aspects. However, we have provided novel information on these genetic relationships. For example, inconsistent with the previous observation, and consistent with our preliminary study using the chloroplast rbcL genes, our analysis showed that Feroniella oblata is not nested in Citrus species and is closely related with Feronia limonia. Furthermore, we have shown that Murraya paniculata is similar to Merrillia caloxylon and is dissimilar to Murraya koenigii. We found that "true citrus fruit trees" could be divided into 2 subclusters. One subcluster included Citrus, Fortunella, and Poncirus, while the other cluster included Microcitrus and Eremocitrus. Compared to previous studies, our current study is the most extensive phylogenetic study of Citrus species since it includes 93 accessions. The results indicate that Citrus species can be classified into 3 clusters: a citron cluster, a pummelo cluster, and a mandarin cluster. Although most mandarin accessions belonged to the mandarin cluster, we found some exceptions. We also obtained the information on the genetic background of various species of acid citrus grown in Japan. Because the genus Citrus contains many important accessions

  1. Synergistic interaction and mode of action of Citrus hystrix essential oil against bacteria causing periodontal diseases.

    Science.gov (United States)

    Wongsariya, Karn; Phanthong, Phanida; Bunyapraphatsara, Nuntavan; Srisukh, Vimol; Chomnawang, Mullika Traidej

    2014-03-01

    Citrus hystrix de Candolle (Rutaceae), an edible plant regularly used as a food ingredient, possesses antibacterial activity, but there is no current data on the activity against bacteria causing periodontal diseases. C. hystrix essential oil from leaves and peel were investigated for antibiofilm formation and mode of action against bacteria causing periodontal diseases. In vitro antibacterial and antibiofilm formation activities were determined by broth microdilution and time kill assay. Mode of action of essential oil was observed by SEM and the active component was identified by bioautography and GC/MS. C. hystrix leaves oil exhibited antibacterial activity at the MICs of 1.06 mg/mL for P. gingivalis and S. mutans and 2.12 mg/mL for S. sanguinis. Leaf oil at 4.25 mg/mL showed antibiofilm formation activity with 99% inhibition. The lethal effects on P. gingivalis were observed within 2 and 4 h after treated with 4 × MIC and 2 × MIC, respectively. S. sanguinis and S. mutans were completely killed within 4 and 8 h after exposed to 4 × MIC and 2 × MIC of oil. MICs of tested strains showed 4 times reduction suggesting synergistic interaction of oil and chlorhexidine. Bacterial outer membrane was disrupted after treatment with leaves oil. Additionally, citronellal was identified as the major active compound of C. hystrix oil. C. hystrix leaf oil could be used as a natural active compound or in combination with chlorhexidine in mouthwash preparations to prevent the growth of bacteria associated with periodontal diseases and biofilm formation.

  2. Neutralization of the edema-forming, defibrinating and coagulant effects of Bothrops asper venom by extracts of plants used by healers in Colombia

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    V. Núñez

    2004-07-01

    Full Text Available We determined the neutralizing activity of 12 ethanolic extracts of plants against the edema-forming, defibrinating and coagulant effects of Bothrops asper venom in Swiss Webster mice. The material used consisted of the leaves and branches of Bixa orellana (Bixaceae, Ficus nymphaeifolia (Moraceae, Struthanthus orbicularis (Loranthaceae and Gonzalagunia panamensis (Rubiaceae; the stem barks of Brownea rosademonte (Caesalpiniaceae and Tabebuia rosea (Bignoniaceae; the whole plant of Pleopeltis percussa (Polypodiaceae and Trichomanes elegans (Hymenophyllaceae; rhizomes of Renealmia alpinia (Zingiberaceae, Heliconia curtispatha (Heliconiaceae and Dracontium croatii (Araceae, and the ripe fruit of Citrus limon (Rutaceae. After preincubation of varying amounts of each extract with either 1.0 µg venom for the edema-forming effect or 2.0 µg venom for the defibrinating effect, the mixture was injected subcutaneously (sc into the right foot pad or intravenously into the tail, respectively, to groups of four mice (18-20 g. All extracts (6.2-200 µg/mouse partially neutralized the edema-forming activity of venom in a dose-dependent manner (58-76% inhibition, with B. orellana, S. orbicularis, G. panamensis, B. rosademonte, and D. croatii showing the highest effect. Ten extracts (3.9-2000 µg/mouse also showed 100% neutralizing ability against the defibrinating effect of venom, and nine prolonged the coagulation time induced by the venom. When the extracts were administered either before or after venom injection, the neutralization of the edema-forming effect was lower than 40% for all extracts, and none of them neutralized the defibrinating effect of venom. When they were administered in situ (sc at the same site 5 min after venom injection, the neutralization of edema increased for six extracts, reaching levels up to 64% for C. limon.

  3. Insecticidal and Feeding Deterrent Effects of Fraxinellone from Dictamnus dasycarpus against Four Major Pests

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    Huixia Liu

    2013-03-01

    Full Text Available Fraxinellone, a well-known and significant naturally occurring compound isolated from Meliaceae and Rutaceae spp. has been widely used as a drug for the treatment of tumors. On the other hand, fraxinellone exhibited a variety of insecticidal activities including feeding-deterrent activity, inhibition of growth, and larvicidal activity. The present study focused on the antifeedant and larvicidal activities of fraxinellone against the larvae of Lepidoptera, including Mythimna separata, Agrotis ypsilon, Plutella xylostella, and one kind of sanitary pest, Culux pipiens pallens. Meanwhile, the ovicidal activities and the effects of fraxinellone on the larval development of M. separata were also observed. The LC50 values of fraxinellone against 3rd instar larvae of M. separata, 2nd instar larvae of P. xylostella and 4th instar larvae of C. pipiens pallens were 15.95/6.43/3.60 × 10−2 mg mL−1, and its AFC50 values against 5th instar larvae of M. separata, 2nd instar larvae of P. xylostella and 2nd instar larvae of A. ypsilon were 10.73/7.93/12.58 mg mL−1, respectively. Compared with the control group, fraxinellone obviously inhibited the pupation rate and the growth of M. separata. Once M. separata was treated with fraxinellone at concentrations of 5.0, 10.0, and 20.0 mg mL−1, respectively, the stages from the larvae to adulthood and the egg hatching duration were prolonged to 1/2/3, and 4/3/4 days, respectively. Additionally, fraxinellone strongly inhibited the development rate and the egg hatch proportion of M. separata.

  4. The Influence of Learning on Host Plant Preference in a Significant Phytopathogen Vector, Diaphorina citri.

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    Dara G Stockton

    Rutaceae in the area being monitored.

  5. Phylogenetic relationships of citrus and its relatives based on matK gene sequences.

    Science.gov (United States)

    Penjor, Tshering; Yamamoto, Masashi; Uehara, Miki; Ide, Manami; Matsumoto, Natsumi; Matsumoto, Ryoji; Nagano, Yukio

    2013-01-01

    The genus Citrus includes mandarin, orange, lemon, grapefruit and lime, which have high economic and nutritional value. The family Rutaceae can be divided into 7 subfamilies, including Aurantioideae. The genus Citrus belongs to the subfamily Aurantioideae. In this study, we sequenced the chloroplast matK genes of 135 accessions from 22 genera of Aurantioideae and analyzed them phylogenetically. Our study includes many accessions that have not been examined in other studies. The subfamily Aurantioideae has been classified into 2 tribes, Clauseneae and Citreae, and our current molecular analysis clearly discriminate Citreae from Clauseneae by using only 1 chloroplast DNA sequence. Our study confirms previous observations on the molecular phylogeny of Aurantioideae in many aspects. However, we have provided novel information on these genetic relationships. For example, inconsistent with the previous observation, and consistent with our preliminary study using the chloroplast rbcL genes, our analysis showed that Feroniella oblata is not nested in Citrus species and is closely related with Feronia limonia. Furthermore, we have shown that Murraya paniculata is similar to Merrillia caloxylon and is dissimilar to Murraya koenigii. We found that "true citrus fruit trees" could be divided into 2 subclusters. One subcluster included Citrus, Fortunella, and Poncirus, while the other cluster included Microcitrus and Eremocitrus. Compared to previous studies, our current study is the most extensive phylogenetic study of Citrus species since it includes 93 accessions. The results indicate that Citrus species can be classified into 3 clusters: a citron cluster, a pummelo cluster, and a mandarin cluster. Although most mandarin accessions belonged to the mandarin cluster, we found some exceptions. We also obtained the information on the genetic background of various species of acid citrus grown in Japan. Because the genus Citrus contains many important accessions, we have

  6. Ethnobotanical survey of medicinal plants used to manage High Blood Pressure and Type 2 Diabetes Mellitus in Bitterfontein, Western Cape Province, South Africa.

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    Davids, Denver; Gibson, Diana; Johnson, Quinton

    2016-12-24

    The aim of this study was to identify and document medicinal plants used to manage High Blood Pressure and Type 2 Diabetes Mellitus in Bitterfontein, Western Cape Province, South Africa. One hundred and twelve (112) respondents were interviewed between August 2014 and September 2015 through semi-structured surveys to gather data on the percentage of people who had been diagnosed with High Blood Pressure and/or Type 2 Diabetes Mellitus and to determine the frequency of medicinal plant and allopathic medicine use. Twelve (12) key respondents were subsequently selected, using a non-probability snowball sampling method. They were interviewed in-depth concerning their plant practices and assisted with plant collection. Twenty-four plant (24) species belonging to 15 families were identified for the management of High Blood Pressure and Type 2 Diabetes Mellitus. The most frequently reported families were Asteraceae (20.8%), Lamiaceae (16.67%), Crassulaceae (8.33%) and Aizoaceae (8.33%). The remaining (45.54%) were evenly split over eleven families- Fabaceae, Amaryllidaceae, Anacardiaceae, Capparaceae, Geraniaceae, Apiaceae, Convolvulaceae, Apocynaceae, Rutaceae, Asphodelaceae and Thymelaeaceae. The most commonly used plant species overall was Lessertia frutescens (96.55%). The most frequently used plant parts included leaves (57.63%) roots/bulbs (15.25%) and stems (11.86%), mostly prepared as infusions or decoctions for oral administration. Medicinal plants are widely used by High Blood Pressure and Type 2 Diabetes Mellitus sufferers. They employ diverse plant species to manage both conditions. In addition, some sufferers often use prescribed allopathic medication, as well as medicinal plants, but at different intervals. Despite high usage the plants identified are not currently threatened (Red Data list status: least concern). Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  7. Uso tradicional de plantas medicinales en la vereda San Isidro, municipio de San José de Pare-Boyacá: un estudio preliminar usando técnicas cuantitativas

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    Jarvis Yamith Toscano González

    2006-07-01

    Full Text Available Se evaluó cuantitativamente el uso de plantas medicinales con el propósito de documentar la importancia relativa de dichas especies y estimar el estado actual del conocimiento tradicional de las comunidades campesinas de la vereda San Isidro, municipio de San José de Pare, Boyacá. Esta zona en particular, se considera un sector agrícola de alta confluencia y denota una apropiación del conocimiento de sus pobladores en el uso y manejo de las especies vegetales de la región. Se aplicaron aleatoriamente 180 encuestas a personas entre los 20 y 80 años, en donde se investigó respecto al uso y manejo de las plantas. Se aplicaron entrevistas programadas a aquellas personas que manejan en gran parte la demanda de plantas medicinales en la región. Los datos obtenidos a partir del consenso de informantes, fueron analizados mediante el índice de valor de uso (IVU y el nivel de uso significativo de TRAMIL. Se registraron 84 especies con 78 géneros, agrupadas en 42 familias botánicas entre medicinales, aromáticas y mágico-religiosas; las familias mejor representadas fueron Lamiaceae, Asteraceae, Apiaceae, Solanaceae y Rutaceae. Se reportaron 10 especies con un mayor nivel de uso significativo y 25 especies con un mayor IVU. La documentación de los usos de las plantas medicinales en el área de influencia, revela que el conocimiento tradicional continúa profundamente arraigado entre la comunidad y se mantiene el saber popular a manos de curanderos y madres cabeza de familia.

  8. Efficacy and safety of Citrus sudachi peel in obese adults: A randomized, double-blind, pilot study

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    Masashi Akaike

    2014-07-01

    Full Text Available Objective: This study was undertaken to explore the efficacy and safety of Citrus sudachi peel for metabolic risk factors in obese male and female adults. Background: Citrus sudachi Hort. ex Shirai (Rutaceae, called “sudachi”, is a small, round, green citrus fruit that is mainly cultivated in Tokushima Prefecture in Japan. Our group reported that Citrus sudachi peel powder improved glucose tolerance and dyslipidemia in Zucher-fatty rats and reduced hyperglycemia and hypertriglyceridemia in GK diabetic rats. Materials and Methods: We conducted a randomized, double-blind, placebo-controlled trial in 40 participants with abdominal obesity and metabolic risk factors including hypertension, impaired glucose tolerance and elevated triglyceride levels. Participants were randomized to receive either tablets that contained 1.3 g dried Citrus sudachi peel powder or placebo tablets for 12 weeks. The sudachi peel group included 14 males and 5 females with a mean age of 54.5 years, and the placebo group included 18 males and 2 females with a mean age of 51.9 years. Results: Physical status including body weight, waist circumference and blood pressure and laboratory markers including metabolic parameters were not different at any observation point between the two groups. However, among participants with serum triglyceride levels of more than 120 mg/dl, body weight, waist circumference and serum triglyceride levels were significantly decreased at several observation points after the start of treatment in the sudachi peel group but not in the placebo group. No serious adverse events were observed in the sudachi peel group. Conclusions: Citrus sudachi peel has the potential effect to safely improve abdominal obesity and lower serum levels of TG in obese individuals with hypertriglyceridemia. A large-scale randomized, double-blind clinical study targeting subjects with both abdominal obesity and high TG levels is needed to confirm the metabolic effects of

  9. A draft of the genome and four transcriptomes of a medicinal and pesticidal angiosperm Azadirachta indica

    Science.gov (United States)

    2012-01-01

    Background The Azadirachta indica (neem) tree is a source of a wide number of natural products, including the potent biopesticide azadirachtin. In spite of its widespread applications in agriculture and medicine, the molecular aspects of the biosynthesis of neem terpenoids remain largely unexplored. The current report describes the draft genome and four transcriptomes of A. indica and attempts to contextualise the sequence information in terms of its molecular phylogeny, transcript expression and terpenoid biosynthesis pathways. A. indica is the first member of the family Meliaceae to be sequenced using next generation sequencing approach. Results The genome and transcriptomes of A. indica were sequenced using multiple sequencing platforms and libraries. The A. indica genome is AT-rich, bears few repetitive DNA elements and comprises about 20,000 genes. The molecular phylogenetic analyses grouped A. indica together with Citrus sinensis from the Rutaceae family validating its conventional taxonomic classification. Comparative transcript expression analysis showed either exclusive or enhanced expression of known genes involved in neem terpenoid biosynthesis pathways compared to other sequenced angiosperms. Genome and transcriptome analyses in A. indica led to the identification of repeat elements, nucleotide composition and expression profiles of genes in various organs. Conclusions This study on A. indica genome and transcriptomes will provide a model for characterization of metabolic pathways involved in synthesis of bioactive compounds, comparative evolutionary studies among various Meliaceae family members and help annotate their genomes. A better understanding of molecular pathways involved in the azadirachtin synthesis in A. indica will pave ways for bulk production of environment friendly biopesticides. PMID:22958331

  10. Inhibitory effects of furanocoumarin derivatives in Kampo extract medicines on P-glycoprotein at the blood-brain barrier.

    Science.gov (United States)

    Iwanaga, Kazunori; Yoneda, Shinji; Hamahata, Yukimi; Miyazaki, Makoto; Shibano, Makio; Taniguchi, Masahiko; Baba, Kimiye; Kakemi, Masawo

    2011-01-01

    Furanocoumarin derivatives, known as components of grapefruit juice, showing inhibitory effects against P-glycoprotein (P-gp) in the intestine are also contained in the plants of rutaceae and umbelliferae families, which are used as components of Kampo extract medicines. In this study, we investigated the inhibitory effects of byakangelicol and rivulobirin A, known as furanocoumarins showing P-gp inhibitory effect using Caco-2 monolayer, against P-gp at the blood-brain barrier (BBB) under both in vitro and in vivo conditions. First we studied the membrane permeability of furanocoumarins to clarify whether they can be absorbed from the intestine. Both furanocoumarins showed high permeability through the Caco-2 monolayer, suggesting that they can easily reach the systemic circulation after oral administration. Then, we evaluated the effect of these furanocoumarins on the uptake of calcein acetoxymethyl ester (calcein-AM), a P-gp substrate, into bovine brain microvascular endothelial cells (BBMEC). Both furanocoumarins significantly increased the uptake amount of calcein-AM into BBMEC by the inhibition of P-gp at the BBB in vitro. Next we also investigated the P-gp inhibitory effect of these furanocoumarins at the rat BBB in vivo using verapamil as a P-gp substrate. Both furanocoumarins increased the B/P ratio of verapamil compared to the control, even under in vivo conditions; however, the extent of the inhibitory effect was much lower than in vitro condition. In conclusion, byakangelicol and rivulobirin A may inhibit P-gp expressed at the BBB even under in vivo conditions. Further studies using Kampo extract medicines under in vivo condition are necessary for safe drug therapy.

  11. Antimicrobial activity of Ulopterol isolated from Toddalia asiatica (L.) Lam.: a traditional medicinal plant.

    Science.gov (United States)

    Karunai Raj, M; Balachandran, C; Duraipandiyan, V; Agastian, P; Ignacimuthu, S

    2012-03-06

    The leaves of Toddalia asiatica (L.) Lam. (Rutaceae) are widely used in folk medicine in India to treat various ailments like cough, malaria, indigestion, influenza lung diseases and rheumatism, fever, stomach ailments, cholera and diarrhea. In our earlier communication we have reported the antimicrobial study on the various extracts of the leaves and the isolation and identification of Flindersine, a quinolone alkaloid as the major active principle. In the present study, we report the antibacterial and antifungal activities of Ulopterol, a coumarin isolated as another major active antimicrobial principle. The leaves were successively extracted with hexane, chloroform, ethyl acetate, methanol and water. The extracts were studied for their antimicrobial activity against selected bacteria and fungi by using disc-diffusion method. The ethyl acetate extract which was found to possess highest antimicrobial activity was subjected to activity guided fractionation by column chromatography over silica gel. This resulted in the isolation of the coumarin, Ulopetrol, an active principle besides Flindersine which was reported by us earlier. The structure of the compound was elucidated using physical and spectroscopic data. Flindersine and Ulopterol were quantified by HPLC. Ulopterol showed activity against the bacteria viz. Staphylococcus epidermidis, Enterobacter aerogenes, Shigella flexneri, Klebsiella pneumoniae (ESBL-3967), Escherichia coli (ESBL-3984) and fungi viz. Aspergillus flavus, Candida krusei and Botrytis cinerea. Quantification by HPLC showed the content of Flindersine and Ulopterol to be 0.361% and 0.266% respectively on dry weight basis of the leaves. Ethyl acetate extract (successive extraction) contained Ulopterol, a coumarin, besides Flindersine, a quinolone alkaloid, as a major active principle in the antimicrobial studies. This is the first report of the antimicrobial activity of Ulopterol and also its first report from the plant. Copyright © 2012

  12. Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis

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    Xichun Wang

    2018-01-01

    Full Text Available Berberine hydrochloride is an isoquinoline type alkaloid extracted from Berberidaceae, Rutaceae, and other plants. Previous reports have shown that berberine hydrochloride has anti-inflammatory properties. However, the underlying molecular mechanisms remain unclear. In this study, a lipopolysaccharide- (LPS- induced murine model of mastitis was established to explore the anti-inflammatory action of berberine hydrochloride. Sixty mice that had been lactating for 5–7 days were randomly divided into six groups, including control, LPS, three berberine hydrochloride treatment groups (5, 10, and 20 mg/kg, and a dexamethasone (DEX (5 mg/kg group. Berberine hydrochloride was administered intraperitoneally 1 h before and 12 h after LPS-induced mastitis, and all mice were sacrificed 24 h after LPS induction. The pathological and histopathological changes of the mammary glands were observed. The concentrations and mRNA expressions of TNF-α, IL-1β, and IL-6 were measured by ELISA and qRT-PCR. The activation of TLR4 and NF-κB signaling pathways was analyzed by Western blot. Results indicated that berberine hydrochloride significantly attenuated neutrophil infiltration and dose-dependently decreased the secretion and mRNA expressions of TNF-α, IL-1β, and IL-6 within a certain range. Furthermore, berberine hydrochloride suppressed LPS-induced TLR4 and NF-κB p65 activation and the phosphorylation of I-κB. Berberine hydrochloride can provide mice robust protection from LPS-induced mastitis, potentially via the TLR4 and NF-κB pathway.

  13. Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis

    Science.gov (United States)

    Feng, Shibin; Ding, Nana; He, Yanting; Li, Cheng; Li, Manman; Ding, Xuedong; Ding, Hongyan; Li, Jinchun

    2018-01-01

    Berberine hydrochloride is an isoquinoline type alkaloid extracted from Berberidaceae, Rutaceae, and other plants. Previous reports have shown that berberine hydrochloride has anti-inflammatory properties. However, the underlying molecular mechanisms remain unclear. In this study, a lipopolysaccharide- (LPS-) induced murine model of mastitis was established to explore the anti-inflammatory action of berberine hydrochloride. Sixty mice that had been lactating for 5–7 days were randomly divided into six groups, including control, LPS, three berberine hydrochloride treatment groups (5, 10, and 20 mg/kg), and a dexamethasone (DEX) (5 mg/kg) group. Berberine hydrochloride was administered intraperitoneally 1 h before and 12 h after LPS-induced mastitis, and all mice were sacrificed 24 h after LPS induction. The pathological and histopathological changes of the mammary glands were observed. The concentrations and mRNA expressions of TNF-α, IL-1β, and IL-6 were measured by ELISA and qRT-PCR. The activation of TLR4 and NF-κB signaling pathways was analyzed by Western blot. Results indicated that berberine hydrochloride significantly attenuated neutrophil infiltration and dose-dependently decreased the secretion and mRNA expressions of TNF-α, IL-1β, and IL-6 within a certain range. Furthermore, berberine hydrochloride suppressed LPS-induced TLR4 and NF-κB p65 activation and the phosphorylation of I-κB. Berberine hydrochloride can provide mice robust protection from LPS-induced mastitis, potentially via the TLR4 and NF-κB pathway.

  14. Anti-inflammatory activity of Choisya ternata Kunth essential oil, ternanthranin, and its two synthetic analogs (methyl and propyl N-methylanthranilates.

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    Mariana Martins Gomes Pinheiro

    Full Text Available Choisya ternata Kunth (Rutaceae is native to North America where it is popularly known as "Mexican orange". In this study, the anti-inflammatory effects of the essential oil (EO obtained from the leaves of C. ternata, one of its minor components (ternanthranin-ISOAN and its two synthetic analogues (methyl and propyl N-methylanthranilate--MAN and PAN were evaluated. Mice pretreated with the EO (EO obtained from C. ternata leaves (3-100 mg/kg, p.o., ISOAN, MAN or PAN (1-30 mg/kg, p.o. and the reference drugs, morphine (1 mg/kg, p.o. and acetylsalicylic acid (ASA, 100 mg/kg, p.o., were evaluated in inflammation models such as formalin and subcutaneous air pouch models, with measurement of cell migration, exudate volume, protein extravasation, nitric oxide and pro-inflammatory cytokines. The EO from C. ternata significantly inhibited the time that the animals spent licking the formalin-injected paw in the second phase of the model at their higher doses (30 and 100 mg/kg, respectively. An inhibition of the inflammatory reaction induced after subcutaneous carrageenan injection into air pouch was also observed. In this model, the EO significantly reduced cell migration, exudate volume, protein extravased, and the increase in levels of inflammatory mediators (nitric oxide, TNF-α and IL-1β. ISOAN, MAN and PAN behaved in the same fashion at much smaller doses. Also, these molecules were able to show significant effects in the reduction of paw edema (at all tested doses when the phlogistic agent was carrageenan, bradykinin, 5-HT, PGE2, C48/80 or 12-O-tetradecanoylphorbol-acetate (TPA. None of the tested doses had any effect in reducing histamine-induced edema. Our results indicate that the EO from C. ternata and anthranilate derivatives demonstrates an anti-inflammatory effect.

  15. Cape plants: corrections and additions to the flora. 1

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    P. Goldblatt

    2005-08-01

    Full Text Available Comprising an area of ± 90 000 km:, less than 5% of the land surface of the southern African subcontinent, the Cape Floristic Region (CFR is one of the world’s richest areas for plant species diversity. A recent synoptic flora for the Region has established a new base line for an accurate assessment of the flora. Here we document corrections and additions to the flora at family, genus and species ranks. As treated in Cape plants, which was completed in 1999. the flora comprised 173 families (five endemic, 988 genera (160 endemic: 16.2%, and 9 004 species (6 192 endemic: 68.8%. Just four years later, a revised count resulting from changes in the circumscriptions of families and genera, and the discovery of new species or range extensions of species, yields an estimate of 172 families (four endemic, 992 genera (162 endemic: 16.3% and 9 086 species (6 226: 68.5% endemic. Of these, 948 genera and 8 971 species are seed plants. The number of species packed into so small an area is remarkable for the temperate zone and compares favourably with species richness for areas of compa­rable size in the wet tropics. The degree of endemism is also remarkable for a continental area. An unusual family compo­sition includes, in descending order of size, based on species number. Asteraceae. Fabaceae. Iridaceae. Ericaceae. Aizoaceae, Scrophulariaceae. Proteaceae. Restionaceae, Rutaceae. and Orchidaceae. Disproportionate radiation has resulted in 59.1% of the species falling in the 10 largest families and 74.6% in the largest 20 families. Thirteen genera have more than 100 species and the 20 largest genera contribute some 31.5% of the total species number.

  16. Hepatoprotective effect of Aegle marmelos augmented with piperine co-administration in paracetamol model

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    Deepti Rathee

    Full Text Available ABSTRACT The current study explored hepatoprotective effect of Aegle marmelos (L. Corrêa, Rutaceae, leaves extract. Potentiation of A. marmelos hepatoprotective effect with piperine co-administration was also explored. Wistar rats were randomly divided into seven groups: (i normal control, (ii paracetamol group, (iii silymarin group, (iv extract-25 group (25 mg/kg body, (v extract-50 group: (50 mg/kg, (vi extract-100 group (100 mg/kg and (vii extract-25 + piperine group. Hepatotoxicity was induced by administering paracetamol orally in a dose of 400 mg/kg for seven days. The drugs were administered 30 min prior to paracetamol administration and continued for seven days. Animals were ‘sacrificed’ at the end of treatment and serum was collected for evaluating alkaline phosphatase, bilirubin, lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase IL-10 and TNF-α levels. Liver homogenates were used for determination of oxidative stress (malondialdehyde, reduced glutathione, superoxide dismutase, catalase, glutathione reductase, GSH-S-transferase, glutathione peroxidase and glucose-6-phosphate dehydrogenase. Serum biochemical markers were significantly higher in paracetamol group as compared to normal control group. Significant increase in oxidative stress parameters and inflammatory mediators was also observed. Treatment with A. marmelos curtailed the toxic effects of paracetamol in a dose dependent fashion. 100 mg/kg dose of A. marmelos was found to be most hepatoprotective. The results of extract-100 group were comparable to silymarin group. Low dose of A. marmelos i.e., 25 mg/kg was combined with piperine to evaluate potentiation of hepatoprotective effects of A. marmelos. Piperine co-administration potentiated the hepatoprotective effects, because the combination group results were comparable to high dose A. marmelos group. A. marmelos exerts hepatoprotective activity through its antioxidant and anti

  17. Insecticidal Constituents of Essential Oil Derived from Zanthoxylum armatum against Two Stored-Product Insects.

    Science.gov (United States)

    Wang, Cheng-Fang; Zhang, Wen-Juan; You, Chun-Xue; Guo, Shan-Shan; Geng, Zhu-Feng; Fan, Li; Du, Shu-Shan; Deng, Zhi-Wei; Wang, Yong-Yan

    2015-01-01

    In the course of our search for natural bioactive chemicals and investigations on their insecticidal activities from some medicinal plants growing in China, the essential oil derived from the twigs of Zanthoxylum armatum (Rutaceae) was found to possess strong insecticidal activities against two stored-product insects, Lasioderma serricorne and Tribolium castaneum. A total of 32 constituents of the essential oil were identified by GC and GC-MS analysis, and it revealed (E)-anethole (20.5%), 1,8-cineole (14.0%), 2-tridecanone (12.5%), limonene (9.0%) and piperitone (8.0%) as major components, followed by β-phellandrene (6.3%), β-pinene (5.1%) and 4-terpineol (4.4%). From the essential oil, five compounds were isolated and identified as (E)-anethole, 1,8-cineole, 2-tridecanone, limonene and piperitone. The results of insecticidal bioassays showed that the essential oil of Z. armatum exhibited strong fumigant toxicity towards L. serricorne and T. castaneum with LC50 values of 13.83 and 4.28 mg/L air, respectively, and also possessed contact toxicity against two insect species with LD50 values of 18.74 and 32.16 μg/adult, respectively. Among the active compounds, piperitone performed the strongest fumigant toxicity against L. serricorne (LC50 = 1.21 mg/L air) and contact toxicity against T. castaneum (LD50 = 3.16 μg/adult). 1,8-Cineole, limonene and piperitone showed similar fumigant toxicity against T. castaneum with LC50 values of 5.47, 6.21 and 7.12 mg/L air, respectively. Meanwhile, L. serricorne was the most sensitive to 2-tridecanone (LD50 = 5.74 μg/adult) in the progress of contact toxicity assay.

  18. Análise florística e estrutural de um fragmento de Floresta Ombrófila Mista Montana, situado em Criúva, RS - Brasil.

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    Rubens Marques Rondon Neto

    2010-08-01

    Full Text Available O presente trabalho teve como objetivo conhecer e analisar a composição florística e descrever a estrutura do componente arbóreo de um fragmento de Floresta Ombrófila Mista Montana, localizado no município de Criúva, RS. Em oito parcelas de 10 x 100 m foram inventariados 673 indivíduos com DAP  5 cm, distribuídos em 37 espécies, pertencentes a 32 gêneros e 22 famílias. O valor do índice de diversidade de Shannon foi de 2,768. As famílias que apresentaram maior número de indivíduos amostrados são: Araucariaceae, Myrsinaceae, Euphorbiaceae, Anacardiaceae e Rutaceae. O dossel dessa floresta é dominado pela Araucaria angustifolia e o sub-bosque por espécies das famílias Lauraceae e Myrtaceae. A densidade total encontrada foi de 841,25 indivíduos/ha, com DAP médio de 24,01 cm e altura média de 9,34 m. As cinco espécies que tiveram os maiores valores de importância, em ordem decrescente, foram: Araucaria angustifolia, Sebastiania commersoniana, Lithraea brasiliensis, Zanthoxylum rhoifolium e Myrcia sp.. O fragmento apresenta uma considerável diversidade florística de espécies, apesar das perturbações sofridas no passado. Para que a vegetação atinja as características florísticas e estruturais próximas às da vegetação original é preciso evitar a presença de bovinos e eqüinos na área e explorações da floresta.

  19. ANÁLISE FLORÍSTICA E ESTRUTURAL DE UM FRAGMENTO DE FLORESTA OMBRÓFILA MISTA MONTANA, SITUADO EM CRIÚVA, RS - BRASIL

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    Rubens Marques Rondon Neto

    2001-10-01

    Full Text Available O presente trabalho teve como objetivo conhecer e analisar a composição florística e descrever a estrutura do componente arbóreo de um fragmento de Floresta Ombrófila Mista Montana, localizado no município de Criúva, RS. Em oito parcelas de 10 x 100 m foram inventariados 673 indivíduos com DAP  5 cm, distribuídos em 37 espécies, pertencentes a 32 gêneros e 22 famílias. O valor do índice de diversidade de Shannon foi de 2,768. As famílias que apresentaram maior número de indivíduos amostrados são: Araucariaceae, Myrsinaceae, Euphorbiaceae, Anacardiaceae e Rutaceae. O dossel dessa floresta é dominado pela Araucaria angustifolia e o sub-bosque por espécies das famílias Lauraceae e Myrtaceae. A densidade total encontrada foi de 841,25 indivíduos/ha, com DAP médio de 24,01 cm e altura média de 9,34 m. As cinco espécies que tiveram os maiores valores de importância, em ordem decrescente, foram: Araucaria angustifolia, Sebastiania commersoniana, Lithraea brasiliensis, Zanthoxylum rhoifolium e Myrcia sp.. O fragmento apresenta uma considerável diversidade florística de espécies, apesar das perturbações sofridas no passado. Para que a vegetação atinja as características florísticas e estruturais próximas às da vegetação original é preciso evitar a presença de bovinos e eqüinos na área e explorações da floresta.

  20. COMPOSIÇÃO FLORÍSTICA E ESTRUTURA DA COMUNIDADE ARBÓREA DE UM FRAGMENTO FLORESTAL NO MUNICÍPIO DE SANTA MARIA-BRASIL

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    Leandro W. Charão

    1999-06-01

    Full Text Available O presente estudo objetiva descrever a composição florística e a estrutura de uma comunidade arbórea em um fragmento florestal e discutir algumas características dendrométricas das espécies componentes do mesmo. Esta comunidade enquadra-se na região da Floresta Estacional Decidual, estando localizada no Município de Santa Maria, na região da Depressão Central do Estado do RS. Foram locadas, de maneira sistemática, 18 parcelas de 200 m² no interior do fragmento. Na área amostrada foram mensuradas 56 espécies vegetais com CAP ³ 15 cm, distribuídas em 46 gêneros e 28 famílias botânicas. As famílias Rutaceae, Rubiaceae, Flacourtiaceae e Myrsinaceae apresentaram as maiores riquezas em relação ao número de espécies e indivíduos. Helietta apiculata, Casearia sylvestris, Faramea marginata, Myrsine umbellata, Chomelia obtusa e Cabralea canjerana foram as espécies mais importantes da comunidade vegetal estudada. As alturas variaram de 3,5 m, para árvores do estrato inferior até 22 m, para árvores emergentes. A maior parte das árvores vegetam em baixa competição, pois apresentam relação h/d igual ou inferior a 1. Pela distribuição das freqüências das circunferências, verifica-se que 42,25% dos indivíduos tem CAP entre 15 e 25 centímetros e que somente 4,25% tem CAP superior a 65 cm.

  1. Plantas útiles en la medicina tradicional de Malpasito-Huimanguillo, Tabasco, México

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    Edelia Claudina Villarreal-Ibarra

    2015-07-01

    Full Text Available En México las plantas medicinales son el recurso terapéutico por excelencia en la medicina tradicional, es rescatable en gran medida por constituir un importante elemento al combinar el conocimiento popular con el científi co para el tratamiento de diversas enfermedades en la atención primaria de la salud. El presente estudio documenta el conocimiento tradicional de las plantas medicinales en la comunidad de Malpasito, dentro de la Reserva Ecológica de Agua Selva en Huimanguillo, Tabasco, México. Se incluye un inventario de plantas medicinales de la región, información sobre el uso terapéutico, la forma de preparación y parte vegetal utilizada. La información se recabó mediante entrevistas estructuradas TRAMIL. Se registraron 128 especies de plantas medicinales empleadas en la comunidad distribuidas en 63 familias botánicas cuyas especies dominantes pertenence a: Asteraceae (10, Leguminosae y Lamiaceae (siete, Verbeneaceae (cinco, Rutaceae y Solanaceae (cuatro. Se reportan 13 especies medicinales con nivel de uso signifi cativo superior al 20%. Los usos medicinales se agruparon y clasifi caron en 17 categorías, en orden de prioridad: desórdenes del sistema digestivo, genitourinario y endócrino, seguidos de las infecciones y desórdenes del sistema respiratorio. Los resultados sugieren la necesidad de futuros estudios de valoración científica para las especies locales empleadas para el tratamiento de alguna enfermedad.

  2. Snakebites and ethnobotany in the northwest region of Colombia. Part III: neutralization of the haemorrhagic effect of Bothrops atrox venom.

    Science.gov (United States)

    Otero, R; Núñez, V; Barona, J; Fonnegra, R; Jiménez, S L; Osorio, R G; Saldarriaga, M; Díaz, A

    2000-11-01

    Thirty-one of 75 extracts of plants used by traditional healers for snakebites, had moderate or high neutralizing ability against the haemorrhagic effect of Bothrops atrox venom from Antioquia and Chocó, north-western Colombia. After preincubation of several doses of every extract (7.8-4000 microg/mouse) with six minimum haemorrhagic doses (10 microg) of venom, 12 of them demonstrated 100% neutralizing capacity when the mixture was i.d. injected into mice (18-20 g). These were the stem barks of Brownea rosademonte (Caesalpiniaceae) and Tabebuia rosea (Bignoniaceae); the whole plants of Pleopeltis percussa (Polypodiaceae), Trichomanes elegans (Hymenophyllaceae) and Senna dariensis (Caesalpiniaceae); rhizomes of Heliconia curtispatha (Heliconiaceae); leaves and branches of Bixa orellana (Bixaceae), Philodendron tripartitum (Araceae), Struthanthus orbicularis (Loranthaceae) and Gonzalagunia panamensis (Rubiaceae); the ripe fruits of Citrus limon (Rutaceae); leaves, branches and stem of Ficus nymphaeifolia (Moraceae). Extracts of another 19 species showed moderate neutralization (21-72%) at doses up to 4 mg/mouse, e.g. the whole plants of Aristolochia grandiflora (Aristolochiaceae), Columnea kalbreyeriana (Gesneriaceae), Sida acuta (Malvaceae), Selaginella articulata (Selaginellaceae) and Pseudoelephantopus spicatus (Asteraceae); rhizomes of Renealmia alpinia (Zingiberaceae); the stem of Strychnos xinguensis (Loganiaceae); leaves, branches and stems of Hyptis capitata (Lamiaceae), Ipomoea cairica (Convolvulaceae), Neurolaena lobata (Asteraceae), Ocimum micranthum (Lamiaceae), Piper pulchrum (Piperaceae), Siparuna thecaphora (Monimiaceae), Castilla elastica (Moraceae) and Allamanda cathartica (Apocynaceae); the macerated ripe fruits of Capsicum frutescens (Solanaceae); the unripe fruits of Crescentia cujete (Bignoniaceae); leaves and branches of Piper arboreum (Piperaceae) and Passiflora quadrangularis (Passifloraceae). When the extracts were independently administered

  3. Effect and mechanism of evodiamine against ethanol-induced gastric ulcer in mice by suppressing Rho/NF-кB pathway.

    Science.gov (United States)

    Zhao, Zhongyan; Gong, Shilin; Wang, Shumin; Ma, Chunhua

    2015-09-01

    Evodiamine (EVD), a major alkaloid compound extracted from the dry unripened fruit Evodia fructus (Evodia rutaecarpa Benth., Rutaceae), has various pharmacological effects. The purpose of the present study was to investigate the possible anti-ulcerogenic potential of EVD and explore the underlying mechanism against ethanol-induced gastric ulcer in mice. Administration of EVD at the doses of 20, 40mg/kg body weight prior to the ethanol ingestion could effectively protect the stomach from ulceration. The gastric lesion was significantly ameliorated in the EVD group compared with that in the model group. Pre-treatment with EVD prevented the oxidative damage and decreased the levels of prostaglandin E2 (PGE2) content, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). In addition, EVD pretreatment markedly increased the serum levels of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT), decreased malonaldehyde (MDA) content in serum and activity of myeloperoxidase (MPO) in stomach tissues compared with those in the model group. In the mechanistic study, significant elevation of Rho, Rho-kinase 1 (ROCK1), ROCK2, cytosolic and nucleic NF-κBp65 expressions were observed in the gastric mucosa group, whereas EVD effectively suppressed the protein expressions of Rho, Rho-kinase 1 (ROCK1), ROCK2, cytosolic and nucleic NF-κBp65 in mice. Moreover, EVD showed protective activity on ethanol-induced GES-1 cells, while the therapeutic effects were not due to its cytotoxity. Taken together, these results strongly indicated that EVD exerted a gastro-protective effect against gastric ulceration. The underlying mechanism might be associated with the improvement of antioxidant and anti-inflammatory status through Rho/NF-κB pathway. Copyright © 2015 Elsevier B.V. All rights reserved.

  4. Neonicotinoid-Induced Mortality of Diaphorina Citri (Hemiptera: Liviidae) is Affected by Route of Exposure.

    Science.gov (United States)

    Langdon, Kevin W; Rogers, Michael E

    2017-10-01

    The use of neonicotinoids in citrus (Rutaceae) has increased substantially to help manage the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of the devastating citrus disease, huanglongbing (HLB). In citrus pest management programs, neonicotinoids are most often applied to the soil as a drench and move through xylem channels from the roots into the foliage. We developed a novel assay to quantify the dose required to kill D. citri following ingestion and compare it with the dose required to kill by contact. The LC50 of the laboratory strain for ingestion of imidacloprid, thiamethoxam, and clothianidin were each approximately 10-fold greater than the respective LC50 by contact exposure. Four field populations were tested to validate comparative exposure of the laboratory strain to imidacloprid and determine the relative susceptibility of field populations to imidacloprid by exposure through ingestion and contact. The contact assay exhibited low (10) RR50 values were observed for the Lake Placid and Lake Alfred populations using the contact and the ingestion method. This research demonstrates that the ingestion assay method described herein is more sensitive in detection of low-level resistance and should be the standard methodology used in monitoring for resistance to systemic insecticides for this global pest. We found D. citri populations with a lower than expected susceptibility to neonicotinoids in the field, which warrants the implementation of resistance management practices to preserve the utility of soil-applied neonicotinoids in citrus. © The Author 2017. Published by Oxford University Press on behalf of Entomological Society of America.

  5. Contrasting amino acid profiles among permissive and non-permissive hosts of Candidatus Liberibacter asiaticus, putative causal agent of Huanglongbing.

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    Mamoudou Sétamou

    Full Text Available Huanglongbing is a devastating disease of citrus. In this study, a comprehensive profile of phloem sap amino acids (AA in four permissive host plants of Candidatus Liberibacter asiaticus (CLas and three non-permissive Rutaceae plants was conducted to gain a better understanding of host factors that may promote or suppress the bacterium. The AA profiles of Diaphorina citri nymphs and adults were similarly analyzed. A total of 38 unique AAs were detected in phloem sap of the various plants and D. citri samples, with phloem sap of young shoots containing more AAs and at higher concentrations than their mature counterparts. All AAs detected in phloem sap of non-permissive plants were also present in CLas -permissive hosts plus additional AAs in the latter class of plants. However, the relative composition of 18 commonly shared AAs varied between CLas -permissive hosts and non-permissive plants. Multivariate analysis with a partial least square discriminant methodology revealed a total of 12 AAs as major factors affecting CLas host status, of which seven were positively related to CLas tolerance/resistance and five positively associated with CLas susceptibility. Most of the AAs positively associated with CLas susceptibility were predominantly of the glutamate family, notably stressed-induced AAs such as arginine, GABA and proline. In contrast, AAs positively correlated with CLas tolerance/resistance were mainly of the serine family. Further analysis revealed that whereas the relative proportions of AAs positively associated with CLas susceptibility did not vary with host developmental stages, those associated with CLas tolerance/resistance increased with flush shoot maturity. Significantly, the proline-to-glycine ratio was determined to be an important discriminating factor for CLas permissivity with higher values characteristic of CLas -permissive hosts. This ratio could be exploited as a biomarker in HLB-resistance breeding programs.

  6. The Influence of Learning on Host Plant Preference in a Significant Phytopathogen Vector, Diaphorina citri.

    Science.gov (United States)

    Stockton, Dara G; Martini, Xavier; Patt, Joseph M; Stelinski, Lukasz L

    2016-01-01

    Although specialist herbivorous insects are guided by innate responses to host plant cues, host plant preference may be influenced by experience and is not dictated by instinct alone. The effect of learning on host plant preference was examined in the Asian citrus psyllid, Diaphorina citri; vector of the causal agent of citrus greening disease or huanglongbing. We investigated: a) whether development on specific host plant species influenced host plant preference in mature D. citri; and b) the extent of associative learning in D. citri in the form of simple and compound conditioning. Learning was measured by cue selection in a 2-choice behavioral assay and compared to naïve controls. Our results showed that learned responses in D. citri are complex and diverse. The developmental host plant species influenced adult host plant preference, with female psyllids preferring the species on which they were reared. However, such preferences were subject to change with the introduction of an alternative host plant within 24-48 hrs, indicating a large degree of experience-dependent response plasticity. Additionally, learning occurred for multiple sensory modalities where novel olfactory and visual environmental cues were associated with the host plant. However, males and females displayed differing discriminatory abilities. In compound conditioning tasks, males exhibited recognition of a compound stimulus alone while females were capable of learning the individual components. These findings suggest D. citri are dynamic animals that demonstrate host plant preference based on developmental and adult experience and can learn to recognize olfactory and visual host plant stimuli in ways that may be sex specific. These experience-based associations are likely used by adults to locate and select suitable host plants for feeding and reproduction and may suggest the need for more tailored lures and traps, which reflect region-specific cultivars or predominate Rutaceae in the area

  7. An ethnobotany of Western Cape Rasta bush medicine.

    Science.gov (United States)

    Aston Philander, Lisa

    2011-11-18

    This descriptive ethnobiological research is the first documentation of the materials utilized in the pharmacopeia of a novel group of herbalists, Rasta bush doctors, found in the botanically diverse Western Cape of South Africa. This article suggests that medicinal plants used by bush doctors unite the disparate ethnomedicines found in South Africa. Ethnospecies name and parts used were recorded during detailed inventories of 39 bush doctors. Collection of voucher specimens for botanical identification occurred in 15 locations. Herbal remedies were classified into use categories and were compared to historical ethnobotanical literature to ascertain previous cultural affiliations. There were 205 ethnospecies found in the Rastafari ethnobiology, 181 were used medicinally. Ethnospecies belong to 71 plant families and 71% belonged to six plant families: Rutaceae (13), Asteraceae (13), Apiaceae (9), Lamiaceae (8), Fabaceae (8), and Euphorbiaceae (7). The majority of remedies (49%) were foliage. Medicinal plants treated over 30 ailments including: gastrointestinal symptoms (11%), urogential complaints (11%), skin ailments (9%), and cardiovascular diseases (8%). Bush doctors appropriated remedies traditionally important to Zulu, KhoiSan, European and Xhosa healing traditions. Novel plants and plant utilization were noted for 22 plant species. Use of previously undocumented plant materials as medicinals denote distinct local knowledge including novel Rastafarian utilization of herbs for spiritual and ritual purposes. The range of the largely herbaceous pharmacopeia is narrow compared to the region's highly biodiverse materials and historical records of medicinal use. Bush doctors' experimentation with known herbal remedies illustrates a striking level of cross-cultural adaptation. This syncretic pharmacopeia reflects the cultural diversity of Southern Africa, drawing upon recent invasive species, European influence and traditional herbs used by the KhoiSan, Zulu and Xhosa

  8. Larvicidal activity of lignans and alkaloid identified in Zanthoxylum piperitum bark toward insecticide-susceptible and wild Culex pipiens pallens and Aedes aegypti.

    Science.gov (United States)

    Kim, Soon-Il; Ahn, Young-Joon

    2017-05-04

    The yellow fever mosquito, Aedes aegypti, and the common house mosquito, Culex pipiens pallens, transmit dengue fever and West Nile virus diseases, respectively. This study was conducted to determine the toxicity of the three lignans (-)-asarinin, sesamin and (+)-xanthoxylol-γ,γ-dimethylallylether (XDA), and the alkaloid pellitorine from Zanthoxylum piperitum (Rutaceae) bark to third-instar larvae from insecticide-susceptible C. pipiens pallens and Ae. aegypti as well as wild C. pipiens pallens resistant to deltamethrin, cyfluthrin, fenthion, and temephos. The toxicities of all isolates were compared with those of mosquito larvicide temephos. LC 50 values for each species and their treatments were significantly different from one another when their 95% confidence intervals did not overlap. XDA was isolated from Z. piperitum as a new larvicidal principle. XDA (LC 50 , 0.27 and 0.24 mg/l) was 4, 53, and 144 times and 4, 100, and 117 times more toxic than pellitorine, sesamin, and asarinin toward larvae from susceptible C. pipiens pallens and Ae. aegypti, respectively. Overall, all the isolates were less toxic than temephos (LC 50 , 0.006 and 0.009 mg/l). These constituents did not differ in toxicity to larvae from the two Culex strains. The present finding indicates that the lignans and alkaloid and the insecticides do not share a common mode of larvicidal action or elicit cross-resistance. Naturally occurring Z. piperitum bark-derived compounds, particularly XDA, merit further study as potential mosquito larval control agents or as lead compounds for the control of insecticide-resistant mosquito populations.

  9. Diosas y madres, el arquetipo femenino en Diego Rivera

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    Dina Comisarenco Mirkin

    2010-11-01

    Full Text Available En el presente trabajo se estudia la representación del arquetipo femenino en la obra mural de Diego Rivera que el artista entretejió en sus amplios y complejos programas iconográficos con una extraordinaria imaginación poética. Se postula aquí que por una parte,  dicha representación arquetípica partió de su erudita utilización de símbolos alegóricos  y de alusiones a divinidades femeninas de distintas épocas y lugares, como medio para asegurar la universalidad del contenido histórico de su pintura, y que por otra parte, y de forma esencial,  el origen de sus poderosas imágenes femeninas, se remonta a algunas de las experiencias vitales del artista, en particular a aquellas relacionadas con el amor filial, con la maternidad/paternidad, y con las heterodoxas relaciones amorosas que Rivera mantuvo a lo largo de su vida con sobresalientes mujeres de su época a quienes admiró y reconoció a través de sus impactantes retratos y homenajes pictóricos. Se concluye así que el arte mural fue para Rivera no sólo un instrumento de acción política, tal y como es normalmente reconocido por la crítica especializada, sino también, una oportunidad para elaborar temas de carácter subjetivo, en los que paradójicamente alcanza un mayor grado de generalidad y trascendencia que el que caracteriza a su pintura histórica.  Particularmente la expresión simbólica del arquetipo femenino plasmada por Rivera en algunos de sus principales aspectos como la Buena Madre, la Madre Terrible y, la Gran Madre,  tal y como los fue viviendo, construyendo y modificando a través de algunas de sus experiencias vitales más significativas y profundas de su vida personal,  constituye uno de los logros artísticos más eminentes del artista y uno de sus principales aportes iconográficos al arte universal. Sus excelsas imágenes de diosas y madres nos permiten re-conectarnos con el inconciente colectivo, y particularmente con el poder tradicionalmente

  10. Distribution of throughfall and stemflow in multi-strata agroforestry, perennial monoculture, fallow and primary forest in central Amazonia, Brazil

    Science.gov (United States)

    Schroth, Götz; Ferreira da Silva, Luciana; Wolf, Marc-Andree; Geraldes Teixeira, Wenceslau; Zech, Wolfgang

    1999-07-01

    The partitioning of rain water into throughfall, stemflow and interception loss when passing through plant canopies depends on properties of the respective plant species, such as leaf area and branch angles. In heterogeneous vegetation, such as tropical forest or polycultural systems, the presence of different plant species may consequently result in a mosaic of situations with respect to quantity and quality of water inputs into the soil. As these processes influence not only the water availability for the plants, but also water infiltration and nutrient leaching, the understanding of plant effects on the repartitioning of rain water may help in the optimization of land use systems and management practices. We measured throughfall and stemflow in a perennial polyculture (multi-strata agroforestry), monocultures of peach palm (Bactris gasipaes) for fruit and for palmito, a monoculture of cupuaçu (Theobroma grandiflorum), spontaneous fallow and primary forest during one year in central Amazonia, Brazil. The effect on rain water partitioning was measured separately for four useful tree species in the polyculture and for two tree species in the primary forest. Throughfall at two stem distances, and stemflow, differed significantly between tree species, resulting in pronounced spatial patterns of water input into the soil in the polyculture system. For two tree species, peach palm for fruit (Bactris gasipaes) and Brazil nut trees (Bertholletia excelsa), the water input into the soil near the stem was significantly higher than the open-area rainfall. This could lead to increased nutrient leaching when fertilizer is applied close to the stem of these trees. In the primary forest, such spatial patterns could also be detected, with significantly higher water input near a palm (Oenocarpus bacaba) than near a dicotyledonous tree species (Eschweilera sp.). Interception losses were 6·4% in the polyculture, 13·9 and 12·3% in the peach palm monocultures for fruit and for

  11. Evaluation Of Batu Bumbun Sanctuary Ecosystem And Management Strategy Affected By Climate Change In Mahakam Watershed Kutai Kartanegara Indonesia

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    Lariman

    2015-08-01

    Full Text Available Batu Bumbun Sanctuary Middle Mahakam Lake is very important for the fishermen community and Mahakam Irrawaddy Dolphin life concerned to its function as the source of fish and as the feeding ground of Irrawaddy Dolphin Orcaella brevirostris. The changes in the forest function and the climate such as rainfall and water surfaces are predicted to have caused suppression in the ecosystem of Batu Bumbun Sanctuary. The aim of this study is to evaluate the current ecosystem changes of Batu Bumbun Sanctuary and suggest a suitable management strategy as a way to conserve its function. The research was conducted during the dry season April June and rainy season November December 2014 by using survey methods. The measured parameters were including water quality DO pH temperature TSS TDS alkalinity and clarity vegetation composition rainfall water surface elevation and sediment. The data of fish community were analysed by using Shanon-Wiener index. The result showed that 1 The current condition of Batu Bumbun biophysical ecosystem has been experiencing a heavy degradation showed by a high fluctuation of the water surface in two extreme seasons such as the flood in rainy seasons and silt up in the dry season. 2 The vegetation composition in the riverbanks was composed of five species including Bungur Lagerstroemia speciosa Rambai Punai Chaetocarpus Castano carpus Kendikara Dillenia excelsa Kademba Mytragina speciosa and Rengas Gluta renghas. The dominant tree species was Putat Barringtonia asiatica and Perupuk Lophopetalum javanicum. 3 Batu Bumbun Sanctuary has been experiencing a heavy siltation caused by silt material that piles up the weeds during the rainy season. Since 1985 Batu Bumbun was predicted to have rates of silting around 8 cmyear. From those result it can be concluded that Batu Bumbun has been experiencing a heavy degradation showed by a high fluctuation of water surface vegetation composition and heavy siltation. Then the most suitable

  12. A bioclimatic characterization of high elevation habitats in the Alborz mountains of Iran.

    Science.gov (United States)

    Noroozi, Jalil; Körner, Christian

    2018-01-01

    The Alborz mountains in N-Iran at 36° N rise from the Caspian Sea to 5671 m a.s.l., with warm-temperate, winter-deciduous forests in the lower montane belt in northern slopes, and vast treeless terrain at higher elevation. A lack of rainfall (ca. 550 mm at high elevations) cannot explain the absence of trees. Hence, it is an open question, which parts of these mountains belong to the alpine belt. Here we use bioclimatic data to estimate the position of the potential climatic treeline, and thus, define bioclimatologically, what is alpine and what is not. We employed the same miniature data loggers and protocol that had been applied in a Europe-wide assessment of alpine climates and a global survey of treeline temperatures. The data suggest a potential treeline position at ca. 3300 m a.s.l., that is ca. 900 m above the upper edge of the current oak forest, or 450 m above its highest outposts. The alpine terrain above the climatic treeline position shows a temperature regime comparable to sites in the European Alps. At the upper limit of angiosperm life, at 4850 m a.s.l., the growing season lasted 63 days with a seasonal mean root zone temperature of 4.5 °C. We conclude that (1) the absence of trees below 2850 m a.s.l. is clearly due to millennia of land use. The absence of trees between 2850 and 3300 m a.s.l. is either due to the absence of suitable tree taxa, or the only potential regional taxon for those elevations, Juniperus excelsa , had been eradicated by land use as well. (2) These continental mountains provide thermal life conditions in the alpine belt similar to other temperate mountains. (3) Topography and snow melt regimes play a significant role for the structure of the alpine vegetation mosaics.

  13. Phylogenetic origin of limes and lemons revealed by cytoplasmic and nuclear markers.

    Science.gov (United States)

    Curk, Franck; Ollitrault, Frédérique; Garcia-Lor, Andres; Luro, François; Navarro, Luis; Ollitrault, Patrick

    2016-04-01

    The origin of limes and lemons has been a source of conflicting taxonomic opinions. Biochemical studies, numerical taxonomy and recent molecular studies suggested that cultivated Citrus species result from interspecific hybridization between four basic taxa (C. reticulata,C. maxima,C. medica and C. micrantha). However, the origin of most lemons and limes remains controversial or unknown. The aim of this study was to perform extended analyses of the diversity, genetic structure and origin of limes and lemons. The study was based on 133 Citrus accessions. It combined maternal phylogeny studies based on mitochondrial and chloroplastic markers, and nuclear structure analysis based on the evaluation of ploidy level and the use of 123 markers, including 73 basic taxa diagnostic single nucleotide polymorphism (SNP) and indel markers. The lime and lemon horticultural group appears to be highly polymorphic, with diploid, triploid and tetraploid varieties, and to result from many independent reticulation events which defined the sub-groups. Maternal phylogeny involves four cytoplasmic types out of the six encountered in the Citrus genus. All lime and lemon accessions were highly heterozygous, with interspecific admixture of two, three and even the four ancestral taxa genomes. Molecular polymorphism between varieties of the same sub-group was very low. Citrus medica contributed to all limes and lemons and was the direct male parent for the main sub-groups in combination with C. micrantha or close papeda species (for C. aurata, C. excelsa, C. macrophylla and C. aurantifolia--'Mexican' lime types of Tanaka's taxa), C. reticulata(for C. limonia, C. karna and C. jambhiri varieties of Tanaka's taxa, including popular citrus rootstocks such as 'Rangpur' lime, 'Volkamer' and 'Rough' lemons), C. aurantium (for C. limetta and C. limon--yellow lemon types--varieties of Tanaka's taxa) or the C. maxima × C. reticulate hybrid (for C. limettioides--'Palestine sweet' lime types--and C

  14. Extração e fracionamento simultâneo do óleo da castanha-do-Brasil com etanol Extraction and simultaneous separation of the Brazil nuts oil with ethanol

    Directory of Open Access Journals (Sweden)

    Suely Pereira Freitas

    2007-08-01

    Full Text Available O objetivo deste trabalho foi utilizar o etanol comercial para extração e fracionamento simultâneos das frações lipídicas presentes na castanha-do-Brasil (Bertholletia excelsea H.B.K.. O óleo foi obtido a partir da castanha desidratada e moída. O processo foi conduzido na proporção 4:1 solvente/substrato (v.p-1 em banho termostatizado a 65 °C, sob agitação de 30 rpm. A mistura foi filtrada, resfriada a 10 °C e, a seguir, centrifugada para separação das fases: uma fase com consistência de gel (micela rica, contendo 75% de óleo e 25% de etanol, e a outra líquida, contendo 2,4% de óleo e 97,6% de etanol (micela pobre. Pelas características apresentadas, a micela rica tem potencial para ser utilizada no preparo de cremes vegetais como substituto parcial de gorduras hidrogenadas, cujos efeitos biológicos na saúde dos consumidores vêm provocando muitas polêmicas. Além de ser uma alternativa na obtenção de gorduras para a formulação de alimentos mais seguros, a tecnologia proposta poderá ser estendida a diferentes oleaginosas de interesse comercial, eliminando o uso de n-hexano no processamento de óleos e gorduras vegetais.In this work, the extraction and simultaneous separation of lipids from Brazil nuts (Bertholletia excelsa H.B.K. with ethanol were investigated. Brazil nuts were dried and triturated prior to oil extraction. The process was carried out at a rate of 4:1 solvent to substrate (v.w-1. The raw material and ethanol were placed in an erlenmeyer flask and maintained in a temperature-controlled bath at 65 °C and 30 rpm. After 1 hour, the mixture was filtered under a vacuum and the resultant miscella was maintained at 10 °C and centrifuged for phase separation. A rich miscella containing 75% oil and 25% ethanol was obtained presenting a gel consistency while a poor miscella, containing 2.4% oil and 97.6% ethanol, was liquid. The rich miscella presented an important potential to partially replace hydrogenate

  15. Determination of gamma radiation shielding characteristics of some tropical woods

    International Nuclear Information System (INIS)

    Aigbosuria, E. F.

    2011-01-01

    This study compares the shielding characteristics of twenty-two tropical woods by using gamma scintillation detection method. Woods sourced are Anogeisus Leiocarpus(Ayin), Nesogordonia Papverifera(Oro), Entandrophragma Microphyllum(Anunje), Brachystagia Eurycoma(Ako), Cassia Alata(Asunrun), Afzelia Africana(Apa-Igbo), Khaya Grandifoliala(Gedu), Piptadenistrum Africana(Agbonyin), Nanclea Diderrehii(Opepe), Khaya Ivorensis(Oganwo), Chlorophora Excelsa(Iroko), Masonia altissima(Odogi), Entandrophragma Angolense(Ijebo), Altium Sativum(Ayo), Albizia Zygia(Ayunre), Terminalia Superba(Afara), Cordia Millenii(Omo), Melania(Melania), Pycnanthus Angolensis(Akomu), Triplochitons Scleroxylon(Arere), Pine(Pine), Ceiba Pentradra(Araba). The intensities of the emergent radiation were measured, when each of these woods were placed between a scintillation detector and a standard radioactive source. Analysis of result obtained shows an appreciable evidence of radiation attenuation due to the changes in the chemical composition of the woods and the dependence of the attenuation coefficient on energy and densities of these woods. The descending order of attenuation coefficient determined are; Ayin, Oro, Anuje, Ako, Asunrun, Apa-igbo Gedu, Agbonyin, Opepe Oganwo, Iroko Odogi , Ayo, Ayunre, Afara, Omo, Melania, Akomu, Arere, Pine, Araba. For a constant energy of 0.101MeV, the attenuation coefficient are 0.190cm -1 , 0.165cm -1 , 0.163cm -1 , 0.156cm -1 , 0.149cm -1 , 0.143cm -1 , 0.133cm -1 , 0.132cm -1 , 0.127cm -1 , 0.124cm -1 , 0.085cm -1 , 0.123cm -1 , 0.122cm -1 , 0.113cm -1 , 0.101cm -1 , 0.088cm -1 , 0.087cm -1 , 0.086cm -1 , 0.082cm -1 respectively. The wood in descending order of dependence of attenuation coefficient on density are: Ayin,Oro, Anunje,Ako,Asunrun,Apa-Igbo, Gedu, Agbonyin, Opepe, Oganwo, Iroko, Odogi, Ijebo, Ayo, Ayunre, Afara, Omo, Melania, Akomu, Arere, Pine and Araba. The half value layer shows the thickness at various energy regions.

  16. Determination of gamma radiation shielding characteristics of some tropical woods

    Energy Technology Data Exchange (ETDEWEB)

    Aigbosuria, E F [Department of Computer Electronics/Physics, Lead City University, Ibadan (Nigeria)

    2011-10-24

    This study compares the shielding characteristics of twenty-two tropical woods by using gamma scintillation detection method. Woods sourced are Anogeisus Leiocarpus(Ayin), Nesogordonia Papverifera(Oro), Entandrophragma Microphyllum(Anunje), Brachystagia Eurycoma(Ako), Cassia Alata(Asunrun), Afzelia Africana(Apa-Igbo), Khaya Grandifoliala(Gedu), Piptadenistrum Africana(Agbonyin), Nanclea Diderrehii(Opepe), Khaya Ivorensis(Oganwo), Chlorophora Excelsa(Iroko), Masonia altissima(Odogi), Entandrophragma Angolense(Ijebo), Altium Sativum(Ayo), Albizia Zygia(Ayunre), Terminalia Superba(Afara), Cordia Millenii(Omo), Melania(Melania), Pycnanthus Angolensis(Akomu), Triplochitons Scleroxylon(Arere), Pine(Pine), Ceiba Pentradra(Araba). The intensities of the emergent radiation were measured, when each of these woods were placed between a scintillation detector and a standard radioactive source. Analysis of result obtained shows an appreciable evidence of radiation attenuation due to the changes in the chemical composition of the woods and the dependence of the attenuation coefficient on energy and densities of these woods. The descending order of attenuation coefficient determined are; Ayin, Oro, Anuje, Ako, Asunrun, Apa-igbo Gedu, Agbonyin, Opepe Oganwo, Iroko Odogi , Ayo, Ayunre, Afara, Omo, Melania, Akomu, Arere, Pine, Araba. For a constant energy of 0.101MeV, the attenuation coefficient are 0.190cm{sup -1}, 0.165cm{sup -1}, 0.163cm{sup -1}, 0.156cm{sup -1}, 0.149cm{sup -1}, 0.143cm{sup -1}, 0.133cm{sup -1}, 0.132cm{sup -1}, 0.127cm{sup -1}, 0.124cm{sup -1}, 0.085cm{sup -1}, 0.123cm{sup -1}, 0.122cm{sup -1}, 0.113cm{sup -1}, 0.101cm{sup -1}, 0.088cm{sup -1}, 0.087cm{sup -1}, 0.086cm{sup -1}, 0.082cm{sup -1} respectively. The wood in descending order of dependence of attenuation coefficient on density are: Ayin,Oro, Anunje,Ako,Asunrun,Apa-Igbo, Gedu, Agbonyin, Opepe, Oganwo, Iroko, Odogi, Ijebo, Ayo, Ayunre, Afara, Omo, Melania, Akomu, Arere, Pine and Araba. The half value

  17. Razón de un Homenaje Profesor Dr. Gonzalo Esguerra Gómez

    Directory of Open Access Journals (Sweden)

    Mario Camacho Pinto

    1993-04-01

    Full Text Available

    La voluntad unánime y espontánea de la Academia Nacional de Medicina, su Junta Directiva y en especial la Comisión Permanente de Biblioteca y Publicaciones convirtió en grata realidad la idea de consagrar con una edición especial de “MEDICINA” la gallarda existencia de su benemérito ex presidente e ilustre académico quien enalteciera la Institución nacional creada y destinada por la Ley para velar por la salud y la educación médica del pueblo colombiano, así como para fomentar el desarrollo de las ciencias médicas y su práctica ética, lo que en síntesis constituyó el paradigma vital en la obra realizada por Gonzalo Esguerra Gómez.

    Llegó con su bonhomía y estructura profesional sólida a la más altas posiciones de calificado médico, al sitial cimero de hombre de ciencia, de cultor y pionero de la radiología en Colombia, cuyo estelar prestigio rebasó las fronteras patrias.

    En efecto, es admirable y ejemplarizante comprobar que su vocación heredada y su consagración adquirida le permitieron dedicarse precozmente al estudio y cultivo de las ciencias médicas, podría decirse que biológicamente dotado para esta misión, porque aparte de su ambiente privilegiadamente favorable, por su estirpe y tradición médica de alcurnia, tuvo cualidades sobresalientes de inteligencia investigativa, porte señorial y sensibilidad social que le dieron calidad de varón ilustre, exponente genuino de hidalgo santafereño que pudo aunar y manifestar sus excelsas virtudes, noble personalidad de ciudadano ejemplar por su reciedumbre moral e intachable rectitud, aptitud para la cultura universal y el ejercicio cívico que al mismo tiempo traducía en espontáneo fervor por la capacitación científico-docente en alto grado hasta llegar a constituirse en modelo de profesional médico, digno de imitación...

  18. Turning sunlight into stone: the oxalate-carbonate pathway in a tropical tree ecosystem

    Directory of Open Access Journals (Sweden)

    G. Cailleau

    2011-07-01

    Full Text Available An African oxalogenic tree, the iroko tree (Milicia excelsa, has the property to enhance carbonate precipitation in tropical oxisols, where such accumulations are not expected due to the acidic conditions in these types of soils. This uncommon process is linked to the oxalate-carbonate pathway, which increases soil pH through oxalate oxidation. In order to investigate the oxalate-carbonate pathway in the iroko system, fluxes of matter have been identified, described, and evaluated from field to microscopic scales. In the first centimeters of the soil profile, decaying of the organic matter allows the release of whewellite crystals, mainly due to the action of termites and saprophytic fungi. In addition, a concomitant flux of carbonate formed in wood tissues contributes to the carbonate flux and is identified as a direct consequence of wood feeding by termites. Nevertheless, calcite biomineralization of the tree is not a consequence of in situ oxalate consumption, but rather related to the oxalate oxidation inside the upper part of the soil. The consequence of this oxidation is the presence of carbonate ions in the soil solution pumped through the roots, leading to preferential mineralization of the roots and the trunk base. An ideal scenario for the iroko biomineralization and soil carbonate accumulation starts with oxalatization: as the iroko tree grows, the organic matter flux to the soil constitutes the litter, and an oxalate pool is formed on the forest ground. Then, wood rotting agents (mainly termites, saprophytic fungi, and bacteria release significant amounts of oxalate crystals from decaying plant tissues. In addition, some of these agents are themselves producers of oxalate (e.g. fungi. Both processes contribute to a soil pool of "available" oxalate crystals. Oxalate consumption by oxalotrophic bacteria can then start. Carbonate and calcium ions present in the soil solution represent the end products of the oxalate

  19. Disinfestation of copra, desiccated coconut and coffee beans by gamma radiation

    International Nuclear Information System (INIS)

    Manato, E.C.

    1987-08-01

    Nine insect pests were found associated with copra of which copra beetle, Necrobia rufipes, saw-toothed grain beetle, Oryzaephilus surinamensis, red flour beetle, Tribolium castaneum, cigarette beetle, Lasioderma serricorne and tropical warehouse moth, Ephestia cautella were found feeding on this food. While feeding on different coffee beans, coffee bean weevil, Araecerus fasciculatus De Geer showed preference on Arabica, Liberica and Excelsa but not in Robusta coffee beans. For mass rearing, the most suitable medium for copra beetle was desiccated coconut + yeast (2:1) and for coffee bean beetle, it was dried cassava chips + yeast (3:1). The life cycles completed in these food media were 43 to 60 and 42 to 56 days by copra beetle and coffee bean weevil respectively. Irradiation studies on these 2 species of insects showed that the eggs were most sensitive followed by larvae and pupae. A dose of 0.05 kGy prevented adult emergence from irradiated eggs and younger larvae, while doses of 0.10 to 0.25 kGy effected the survival of emerged adults. However, a dose of 0.50 kGy would be effective for the disinfestation of small packages (i.e. 0.25 to 0.50 kg in each) of copra or coffee beans initially infested with immature stages of beetles and weevils respectively. Packaging of irradiated commodities in polypropylene bags particularly those impregnated with permethrin prevented reinfestation by the insect pests. Toxic residues of permethrin in the prolypropylene film resulted in high mortality thereby preventing insect penetration of the packaging materials. Both copra beetles and coffee bean weevils were rather good invaders than penetrators as these species entered into the packages readily through existing openings in jute sack, woven polypropylene sack or flour bag. Organoleptic tests showed no change in aroma, flavour and general acceptability of irradiated coffee beans. In microbial studies it was observed that a dose of 0.6 kGy would eliminate Salmonella

  20. Biocide plants as a sustainable tool for the control of pests and pathogens in vegetable cropping systems

    Directory of Open Access Journals (Sweden)

    Trifone D'Addabbo

    2014-11-01

    Full Text Available Synthetic pesticides have played a major role in crop protection related to the intensification of agricultural systems. In the recent years, environmental side effects and health concerns raised by an indiscriminate use have led the EU to the ban of many synthetic pesticides. As a result of this drastic revision, currently there is a strong need for new and alternative pest control methods. An interesting source of biorational pesticides may be represented by the biocidal compounds naturally occurring in plants as products of the secondary metabolism. Groups of plant secondary metabolites most promising for the development of pesticidal formulations are glucosinolates, saponins, and more generally terpenoid phytoconstituents, such as essential oil and their constituents. Glucosinolates are thioglucosidic secondary metabolites occurring mainly in the Brassicaceae and, at a less extent, in Capparidaceae families. The incorporation of glucosinolate- containing plant material into the soil results in degradation products highly toxic to soilborne pest, pathogens and weeds. This practice, known as biofumigation, may be considered as an ecological alternative to soil toxic fumigants. Plant-derived saponins are triterpene glycosides present in top and root tissues of plant species of the families Leguminosae, Alliaceae, Asteraceae, Polygalaceae and Agavaceae. Saponins and saponin-rich plant materials have been also reported for a biocidal activity on phytoparasites and soilborne plant pathogens. Essential oils are volatile, natural, heterogeneous mixtures of single substances, mainly terpenes and phenolics, formed as secondary metabolites by aromatic plants belonging to several botanical families. Among terpenes, limonoid triterpenes have been demonstrated to possess interesting insecticidal, nematicidal and antifungal properties. Occurrence of these compounds is mainly limited to Meliaceae and Rutaceae. Alkaloids, phenolics, cyanogenic glucosides

  1. Therapeutic effects of Qian-Yu decoction and its three extracts on carrageenan-induced chronic prostatitis/chronic pelvic pain syndrome in rats.

    Science.gov (United States)

    Zhang, Keda; Zeng, Xiaobin; Chen, Yonggang; Zhao, Rong; Wang, Hui; Wu, Jinhu

    2017-01-25

    Qian-Yu decoction (QYD) is a traditional Chinese medicinal recipe composed of Radix astragali (Astragalus membranaceus (Fisch.) Bunge var. mongholicus (Bunge) P.K. Hsiao, Fabaceae ), Herba epimedii (Epimedium brevicornum Maxim., Berberidaceae), Herba leonuri (Leonurus japonicus Houtt., Lamiaceae), Cortex phellodendri (Phellodendron chinense Schneid., Rutaceae) and Radix achyranthis bidentatae (Achyranthes bidentata Bl., Amaranthaceae). This study aimed to evaluate the therapeutic activity of QYD against carrageenan-induced chronic prostatic/chronic pelvic pain syndrome (CP/CPPS) in rats and further elucidate its effective components. Three types of components, total polysaccharides, total flavonoids and total saponins were separately extracted from QYD. Carrageenan-induced CP/CPPS rats were intragastrically administered with lyophilized product of QYD, individual extracts and all the combined forms of extracts for three weeks. Prostatic index (PI) was determined and histopathological analysis was performed. The levels of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), cyclooxygenase-2 (COX-2) and prostaglandin E2 (PEG2) in rat prostate tissues were measured using ELISA. The production of inducible nitric oxide synthase (iNOS) was evaluated by an enzymatic activity assay, and the release of nitric oxide (NO) was determined by a nitrate/nitrite assay. Treatment with QYD significantly ameliorated the histological changes of CP/CPPS rats and reduced the PI by 44.3%, with a marked downregulation of TNF-α (42.8% reduction), IL-1β (45.3%), COX-2 (36.6%), PGE2 (44.2%), iNOS (54.1%) and NO (46.0%). Each of three extracts attenuated the symptom of CP/CPPS, but much more weakly than QYD. The combined administration of three extracts showed efficacy comparable to that of QYD while better than that of any combination of two extracts. A principal component analysis of the six inflammatory mediators as variables indicated that the effects of TS on CP

  2. Cytotoxicity and anti-Leishmania amazonensis activity of Citrus sinensis leaf extracts.

    Science.gov (United States)

    Garcia, Andreza R; Amaral, Ana Claudia F; Azevedo, Mariana M B; Corte-Real, Suzana; Lopes, Rosana C; Alviano, Celuta S; Pinheiro, Anderson S; Vermelho, Alane B; Rodrigues, Igor A

    2017-12-01

    Leishmania amazonensis is the main agent of diffuse cutaneous leishmaniasis, a disease characterized by lesional polymorphism and the commitment of skin surface. Previous reports demonstrated that the Citrus genus possess antimicrobial activity. This study evaluated the anti-L. amazonensis activity of Citrus sinensis (L.) Osbeck (Rutaceae) extracts. Citrus sinensis dried leaves were subjected to maceration with hexane (CH), ethyl acetate (CEA), dichloromethane/ethanol (CD/Et - 1:1) or ethanol/water (CEt/W - 7:3). Leishmania amazonensis promastigotes were treated with C. sinensis extracts (1-525 μg/mL) for 120 h at 27 °C. Ultrastructure alterations of treated parasites were evaluated by transmission electron microscopy. Cytotoxicity of the extracts was assessed on RAW 264.7 and J774.G8 macrophages after 48-h treatment at 37 °C using the tetrazolium assay. In addition, Leishmania-infected macrophages were treated with CH and CD/Et (10-80 μg/mL). CH, CD/Et and CEA displayed antileishmanial activity with 50% inhibitory activity (IC 50 ) of 25.91 ± 4.87, 54.23 ± 3.78 and 62.74 ± 5.04 μg/mL, respectively. Parasites treated with CD/Et (131.2 μg/mL) presented severe alterations including mitochondrial swelling, lipid body formation and intense cytoplasmic vacuolization. CH and CD/Et demonstrated cytotoxic effects similar to that of amphotericin B in the anti-amastigote assays (SI of 2.16, 1.98 and 1.35, respectively). Triterpene amyrins were the main substances in CH and CD/Et extracts. In addition, 80 μg/mL of CD/Et reduced the number of intracellular amastigotes and the percentage of infected macrophages in 63% and 36%, respectively. The results presented here highlight C. sinensis as a promising source of antileishmanial agents.

  3. Medicinal plants used by traditional healers for the treatment of malaria in the Chipinge district in Zimbabwe.

    Science.gov (United States)

    Ngarivhume, Talkmore; Van't Klooster, Charlotte I E A; de Jong, Joop T V M; Van der Westhuizen, Jan H

    2015-01-15

    Because about 50% of the Zimbabwean population is at risk of contracting malaria each year, the majority of people, especially in rural areas, use traditional plant-based medicines to combat malaria. This explorative ethnobotanical survey was undertaken to document how malaria is conceptualized and diagnosed by traditional healers, and to record the medicinal plants used in the prevention and treatment of malaria, their mode of preparation and administration. The research was conducted in three villages in Headman Muzite׳s area and in Chiriga village. These villages are located in the Chipinge district in the Manicaland Province in Zimbabwe.Traditional healers were selected with the assistance of the headman of the Muzite area and a representative of the Zimbabwe National Traditional Healers Association. Semi-structured interviews were conducted with 14 traditional healers from four villages in the Chipinge district in Zimbabwe. In total, 28 plants from 16 plant families are used by the healers who manage malaria with medicinal plants. The most cited plant is Cassia abbreviata Oliv. (Leguminosae) followed by Aristolochia albida Duch (Aristolociaceae) and Toddalia asiatica (L.) Lam. (Rutaceae). Roots (55.3%) are the most common part used. Most of the plant parts used to treat malaria are stored as dried powders in closed bottles. The powders are soaked in hot or cold water and the water extract is taken as the active medicine. The healers consider their medicinal knowledge as a spiritual family heritage. Only 25% of the healers refer the malaria patients that do not respond to their treatment to hospital - they believe evil spirits cause their remedies to failure and they would rather try a different plant or perform a cleansing ceremony. Local knowledge of medicinal plants in the treatment of malaria still exists in all four villages surveyed and traditional healers appear to play an important role in primary health care services in this remote rural area in

  4. Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR.

    Science.gov (United States)

    Oueslati, Amel; Ollitrault, Frederique; Baraket, Ghada; Salhi-Hannachi, Amel; Navarro, Luis; Ollitrault, Patrick

    2016-08-18

    Chloroplast DNA is a primary source of molecular variations for phylogenetic analysis of photosynthetic eukaryotes. However, the sequencing and analysis of multiple chloroplastic regions is difficult to apply to large collections or large samples of natural populations. The objective of our work was to demonstrate that a molecular taxonomic key based on easy, scalable and low-cost genotyping method should be developed from a set of Single Nucleotide Polymorphisms (SNPs) diagnostic of well-established clades. It was applied to the Aurantioideae subfamily, the largest group of the Rutaceae family that includes the cultivated citrus species. The publicly available nucleotide sequences of eight plastid genomic regions were compared for 79 accessions of the Aurantioideae subfamily to search for SNPs revealing taxonomic differentiation at the inter-tribe, inter-subtribe, inter-genus and interspecific levels. Diagnostic SNPs (DSNPs) were found for 46 of the 54 clade levels analysed. Forty DSNPs were selected to develop KASPar markers and their taxonomic value was tested by genotyping 108 accessions of the Aurantioideae subfamily. Twenty-seven markers diagnostic of 24 clades were validated and they displayed a very high rate of transferability in the Aurantioideae subfamily (only 1.2 % of missing data on average). The UPGMA from the validated markers produced a cladistic organisation that was highly coherent with the previous phylogenetic analysis based on the sequence data of the eight plasmid regions. In particular, the monophyletic origin of the "true citrus" genera plus Oxanthera was validated. However, some clarification remains necessary regarding the organisation of the other wild species of the Citreae tribe. We validated the concept that with well-established clades, DSNPs can be selected and efficiently transformed into competitive allele-specific PCR markers (KASPar method) allowing cost-effective highly efficient cladistic analysis in large collections at

  5. Citri Reticulatae Pericarpium (Chenpi): Botany, ethnopharmacology, phytochemistry, and pharmacology of a frequently used traditional Chinese medicine.

    Science.gov (United States)

    Yu, Xin; Sun, Shuang; Guo, Yuyan; Liu, Yan; Yang, Dayu; Li, Guoyu; Lü, Shaowa

    2018-06-28

    Citri Reticulatae Pericarpium (Rutaceae, CRP), commonly called as Chenpi () in Chinese, is most frequently used as a qi-regulating drug in thousands of Chinese medicine prescriptions. CRP is found mainly in major citrus-producing areas such as the Guangdong, Guangxi, Sichuan, Fujian, and Zhejiang Provinces of China. Since thousands of years in China, CRP has been used widely in clinical practice to treat nausea, vomiting, indigestion, anepithymia, diarrhea, cough, expectoration, and so on. Currently, CRP is listed in the Pharmacopoeia of the People's Republic of China. The present paper reviews the botany, ethnopharmacology, phytochemistry, pharmacology, quality control, and toxicology of CRP. Information on CRP was gathered from various sources including the books on traditional Chinese herbal medicine; scientific databases including Elsevier, PubMed, and ScienceDirect; Baidu Scholar; CNKI; and others and from different professional websites. Approximately 140 chemical compounds have been isolated and identified from CRP. Among them, volatile oils and flavonoids are generally considered as the main bioactive and characteristic ingredients. CRP possesses wide pharmacological effects such as having a beneficial effect on the cardiovascular, digestive, and respiratory systems, antitumor, antioxidant, and anti-inflammatory properties; and a protective effect on the liver and nerve. Moreover, hesperidin is chosen as an indicator in the quantitative determination of CRP, and the quantity of aflatoxin in CRP must not exceed the standard limit mentioned in the pharmacopoeia. In brief, CRP has a warming nature, and hence, it can be used in harmony with a lot of medicines. CRP not only exhibits its effects individually but also aids other medicines exhibit a better effect. CRP can be consumed with tea, food, alcohol, and medicine. Irrespective of the form it is being consumed, CRP not only shows a synergistic effect but also has strengths on its own. Modern pharmacological

  6. Traditional alcoholic beverages and their value in the local culture of the Alta Valle del Reno, a mountain borderland between Tuscany and Emilia-Romagna (Italy).

    Science.gov (United States)

    Egea, Teresa; Signorini, Maria Adele; Ongaro, Luca; Rivera, Diego; Obón de Castro, Concepción; Bruschi, Piero

    2016-06-22

    Traditional alcoholic beverages (TABs) have only received marginal attention from researchers and ethnobotanists so far, especially in Italy. This work is focused on plant-based TABs in the Alta Valle del Reno, a mountainous area on the border between Tuscany and Emilia-Romagna regions. The aims of our study were to document local knowledge about TABs and to analyze and discuss the distribution of related knowledge within the investigated communities. Field data were collected through semi-structured interviews. The relative importance of each plant species used to prepare TABs was assessed by calculating a general Use Value Index (UV general), a current UV (UV current) and a past UV (UV past). We also assessed personal experience of use by calculating effective and potential UV (UV effective, UV potential). A multivariate analysis was performed to compare ingredients in recipes recorded in the Alta Valle del Reno with those reported for neighboring areas. Forty-six plant species, belonging to 20 families, were recorded. Rosaceae was the most significant family (98 citations, 19 species), followed by Rutaceae (15, 3) and Lamiaceae (12, 4). The most important species was Prunus cerasus L. (UV general = 0.44), followed by Juglans regia L. (0.38), Rubus idaeus L. (0.27) and Prunus spinosa L. (0.22). Species with the highest UV current were Juglans regia (0.254), Prunus cerasus (0.238) and Citrus limon L. (0.159). The highest UV effective values were obtained by Prunus cerasus (0.413), Juglans regia (0.254), Rubus idaeus (0.222) and Citrus limon (0.206). We also discuss the results of the multivariate analysis. TABs proved to occupy an important place in the traditional culture and social life of the studied communities. Moreover, data highlight the local specificity and richness of this kind of tradition in the Alta Valle del Reno, compared to other Italian areas. Some plant ingredients used for TABs have potential nutraceutical and even therapeutic properties

  7. Use and valuation of native and introduced medicinal plant species in Campo Hermoso and Zetaquira, Boyacá, Colombia

    Science.gov (United States)

    2013-01-01

    Background Medicinal plant species contribute significantly to folk medicine in Colombia. However, few local studies have investigated whether species used are introduced or native and whether there is a difference in importance of native and introduced medicinal plant species. The aim of the present study was to describe the use of medicinal plants within two municipalities, Campo Hermoso and Zetaquira, both in the department of Boyacá, Colombia and to assess the importance of native and introduced plants to healers, amateur healers and local people. As local healers including amateur healers have no history of introduced species our working hypotheses (H1-2) were that H1: native and introduced medicinal plant species are of equal importance and H2: healers and amateur healers do not differentiate in their preferences between native and introduced medicinal plant species. Methods Ten villages were included in the study. A combination of quantitative and qualitative methods was used including questionnaires, semi-structured interviews, in- depth interviews, and open talks. Voucher specimens were collected in home gardens and during field walks. For data analysis, we calculated use value indices and Jaccard index and tested for the above hypothesis using Spearman rank-correlation coefficients and Wilcoxon-Mann–Whitney tests. Results Eighty medicinal plant species were described by locals as the most frequently used. Of these, 78 species were taxonomically identified, distributed within 41 families and 74 genera, which included 35 native species and 43 introduced. The highest valued families were: Asteraceae, Lamiaceae, Apiaceae, Rutaceae and Verbenaceae. The species ranked highest according to their Use Values, in both municipalities, were Mentha suaveolens Ehrh., Ambrosia cumanensis Kunth, and Verbena littoralis Kunth. Introduced species were more important than native ones in Zetaquira, while there was no difference in importance in Campo Hermoso. While healers

  8. CARACTERIZAÇÃO FLORÍSTICA, FITOSSOCIOLÓGICA E REGENERAÇÃO NATURAL DO SUB-BOSQUE DA RESERVA GENÉTICA FLORESTAL TAMANDUÁ, DF

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    Salomão João Marcelo de Rezende

    2004-04-01

    Full Text Available O presente trabalho, desenvolvido na Reserva Genética Florestal Tamanduá, DF, teve como objetivo efetuar o levantamento fitossociológico, composição florística e distribuição espacial dos indivíduos das espécies arbóreas ocorrentes no sub-bosque da Reserva, focalizando Cariniana estrellensis (Raddi O. Kuntze, Apuleia leiocarpa (Vogel J. F.Macbr., Copaifera langsdorffii Desf., Anadenanthera macrocarpa (Benth. Brenan, Virola sebifera Aubl., Hymenaea courbaril var. stilbocarpa (Hayne Lee & Lang., Aspidosperma discolor A. St. Hil., Astronium fraxinifolium Schott e Schefflera morototoni B. Maguire, Stey. & Frodin, consideradas prioritárias para conservação in situ. Foram utilizados cinco transectos com 10 m de largura cada, alocados eqüidistantes e de comprimento variável, demarcados perpendicularmente ao curso da drenagem principal. Foram encontrados 21.482 regenerantes ha-1 em 69 espécies vegetais, 53 gêneros e 39 famílias botânicas. Em número de espécies, as famílias que mais se destacaram entre os regenerantes foram, em ordem decrescente, Leguminosae, Rubiaceae, Sapindaceae e Meliaceae. Entre os jovens, as famílias com maiores valor de importância (VI foram pela ordem Meliaceae (32,78 %, Rubiceae (13,92 %, Burseraceae (13,76 %, Rutaceae (8,54 % e Hippocrateaceae (6,36, totalizando 75,36 % de valor de importância e 78,56 % de valor de cobertura. Todas as espécies objeto deste trabalho ocorreram entre os regenerantes, destacando-se Cariniana estrellensis, porém, apenas Copaifera lagsdorffii e Virola sebifera ocorreram entre os indivíduos jovens. O quociente de mistura de 1:3 indicou tratar-se de uma mata rica em espécies, comparativamente a outros tipos florestais. A distribuição espacial dos regenerantes das espécies em questão mostrou padrões diferenciados.

  9. Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers

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    Albaayit SFA

    2016-06-01

    the levels of tumor necrotic factor-alpha and interleukin-6, while the level of interleukin-10 was increased. This study showed that the gastroprotective effect of MECE is achieved through inhibition of gastric juice secretion and ulcer lesion development, stimulation of mucus secretion, elevation of gastric pH, reduction of reactive oxygen species production, inhibition of apoptosis in the gastric mucosa, and modulation of inflammatory cytokines.Keywords: Rutaceae, antiulcer, antioxidant enzymes, histopathology, immunohistochemical proteins, cytokines

  10. Citrus lemon essential oil: chemical composition, antioxidant and antimicrobial activities with its preservative effect against Listeria monocytogenes inoculated in minced beef meat.

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    Ben Hsouna, Anis; Ben Halima, Nihed; Smaoui, Slim; Hamdi, Naceur

    2017-08-03

    Lemon (Citrus limon) is a flowing plant belonging to the Rutaceae family. Citrus plants constitute one of the main valuable sources of essential oil used in foods and medicinal purposes. In this study, we assessed chemical composition, antioxidant and antimicrobial activities of C. limon essential oil (ClEO) with its preservative effect against Listeria monocytogenes inoculated in minced beef meat. Gas chromatography/mass spectrometry (GC-MS) was used to identify the major components of the obtained ClEO. The antioxidant activities of this ClEO were determined according to the β-carotene bleaching assay, as well as by 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. For antimicrobial activity, agar well diffusion method was used and the minimum inhibitory concentrations (MICs) as well as the minimum fungicidal concentrations (MFCs) were determined. The in situ effect of the ClEO was evaluated through physicochemical parameters (pH and thiobarbituric acid reactive substances (TBARS), as well as against L. monocytogenes in minced beef meat model. Twenty one components were identified in the ClEO and the two dominant compounds were limonene (39.74%) and β-Pinene (25.44%). This ClEO displayed an excellent DPPH scavenging ability with an extract concentration providing 50% inhibition (IC 50 ) of 15.056 μg/ml and a strong β-carotene bleaching inhibition after 120 min of incubation with an IC 50 of 40.147 μg/ml. The MICs varied from 0.039 to 1.25 mg/ml for Gram positive bacteria and from 0.25 to 2.5 mg/ml for Gram-negative bacteria. The meat preserving potential of ClEO was investigated against L. monocytogenes. ClEO successfully inhibited development of L. monocytogenes in minced beef meat. The application of ClEO at a 0.06 and 0.312 mg/g, may open new promising opportunities for the prevention of contamination from and growth of pathogenic bacteria, particularly L. monocytogenes, during minced beef meat storage at 4 °C. Additionally, during

  11. Corte foliar e tempo de transplantio para o uso de plântulas do sub-bosque na restauração florestal

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    Ricardo Augusto Gorne Viani

    2012-04-01

    Full Text Available O uso de plântulas da regeneração natural tem sido recomendado como estratégia para produção de mudas visando à restauração florestal, contudo muitos aspectos técnicos desse método ainda carecem de investigação científica. O objetivo deste trabalho foi avaliar o efeito da redução da área foliar e do transplantio imediato na sobrevivência e crescimento de mudas de espécies arbóreas produzidas a partir de plântulas obtidas da regeneração natural. Plântulas de Esenbeckia leiocarpa (Rutaceae, Eugenia ligustrina (Myrtaceae e Maytenus salicifolia (Celastraceae, obtidas em remanescente de vegetação secundária de Floresta Estacional Semidecidual em Bofete, SP, foram extraídas do solo e submetidas aos tratamentos: I redução de 50% da área de cada folha e transplantio imediato; II nenhuma redução de área das folhas e transplantio imediato; III redução de 50% da área de cada folha, manutenção das plântulas em água e transplantio 24 h após a coleta; e IV nenhuma redução de área das folhas, manutenção das plântulas em água e transplantio 24 h após a coleta. As mudas foram avaliadas com relação à sobrevivência e ao crescimento em altura, ao longo de oito meses. Os resultados evidenciaram que nem o corte das folhas ou a manutenção das plântulas dentro de recipientes com água por 24 h antes do transplantio afetaram os parâmetros avaliados. Assim, para as espécies estudadas a redução da área foliar e o transplantio imediato são desnecessários para a produção de mudas em viveiro a partir de plântulas obtidas da regeneração natural.

  12. The local knowledge of medicinal plants trader and diversity of medicinal plants in the Kabanjahe traditional market, North Sumatra, Indonesia.

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    Silalahi, Marina; Nisyawati; Walujo, Eko Baroto; Supriatna, Jatna; Mangunwardoyo, Wibowo

    2015-12-04

    Market is the main place for transactions of medicinal plants and traditional ingredients by local community in the Karo regency, North Sumatra, Indonesia. This is the first study to document the local knowledge of traders on and the diversity of the medicinal plants. The investigation was carried out in the Kabanjahe traditional market, in the Karo regency. The research goal was to reveal the local knowledge, diversity and utilization of medicinal plants, which have been traded in the Kabanjahe traditional market, as a basis for conservation efforts. The study was conducted through ethnobotanical approach using market surveys. All traders of medicinal plants were surveyed applying in-depth interviews and participative observations. Data were analyzed qualitatively using descriptive statistics. The diversity of medicinal plants was expressed in term of the Shannon-Wiener diversity index (H'), whereas the similarity among traders was indicated by Jaccard index (Ji). Traders of medicinal plants stored the simplicia of medicinal plants in chest of drawers, plastic baskets, plastic bags, and in the air by suspending them from the the stall ceilings. We recorded 344 species, 217 genera and 90 families of medicinal plants. Those that were sold mostly belong to Zingeberaceae (20 species), Poaceae (19 species), and Asclepiadaceae (17 species), and the species received high consumers demand, mostly belong to Zingiberaceae, Rutaceae, and Asclepidiaceae. Asclepidiaceae was used to treat diseases like cancer and heart problems. The Shannon-Wiener diversity index of medicinal plants at the Kabanjahe traditional market was high (H'= 5.637). The high Jaccard similarity index (Ji>0.56) suggested that the traders were trading similar species of medicinal plants. Kabanjahe traditional market is the center for the sale of of medicinal plants as traditional ingredients. Several species are well known for their pharmacological properties but others, [such as: Dischidia imbricata (Blume

  13. Metodología para la evaluación del potencial insecticida de especies forestales.

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    Morales Soto León

    2000-06-01

    Full Text Available La diversidad florística de Colombia plantea enormes retos de investigación, con miras a una utilización racional e integral de sus recursos forestales. Las plantas con efectos biocidas utilizables en el control de plagas o enfermedades revisten una singular importancia. El objetivo de este trabajo es plantear una metodología de fácil aplicación, bajo costo y rápidos resultados, que permita acopiar la información necesaria sobre el mayor número de especies con potencial en este sentido. La metodología plantea la siguiente secuencia: selección de las especies vegetales de interés, apoyada en los reportes bibliográficos, conocimiento ancestral y observaciones personales; recolección del material en el campo; preparación del extracto total a partir del material seco; pruebas iniciales con Artemia salina Lech. para detectar actividad biológica, a través de la determinación de la LC50 (las especies con LC50 menores de 1000 ppm se consideran promisorias y ameritan procesos posteriores de fraccionamiento químico, bioensayos con las sustancias más promisorias sobre algún organismo de interés particular y determinación final de los compuestos activos en la planta. La metodología descrita fue empleada en la evaluación del potencial de acción biocida de 5 especies arbóreas o arbustivas, Guarea guidonia (L. Sleumer y Trichia hirta L. (Meliaceae, Machaerium moritzianum Benth. (Fabaceae, Swinglea glutinosa Merrill. (Rutaceae y Mammea americana L. (Clusiaceae. Se utilizó para los bioensayos iniciales el microcrustáceo Artemia salina Leach como indicador del potencial biocida con el fin de seleccionar las dos especies más promisorias a partir de las LC50 obtenidas. Con las dos se realizaron los bioensayos para evaluar la acción fagoinhibidora en la hormiga arriera Atta cephalotes (L., y el posible control de Alconeura sp. (Homoptera: Cicadellidae, insecto chupador que afecta la ceiba verde Pseudobombax septenatum (Jacq. Dugand

  14. Estudo da concordância das citações de uso e importância das espécies e famílias utilizadas como medicinais pela comunidade do bairro Ponta Grossa, Porto Alegre, RS, Brasil

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    Giovana Secretti Vendruscolo

    2006-06-01

    Full Text Available Ferramentas para quantificar dados etnobotânicos estão sendo usadas como complementares aos levantamentos sobre a utilização de plantas por populações. Neste trabalho são utilizadas técnicas para avaliar a concordância das citações de uso e a importância das espécies e famílias para as 51 pessoas entrevistadas no bairro Ponta Grossa, Porto Alegre, Rio Grande do Sul. Para tal, foram utilizados os cálculos de Valor de Uso (UV e a porcentagem corrigida de Concordância quanto aos Usos Principais (CUPc para as 142 espécies mencionadas no levantamento. As espécies Aloe arborescens Mill., Citrus × aurantium L., Achyrocline satureioides (Lam. DC., Foeniculum vulgare Mill, Eugenia uniflora L., Cunila microcephala Benth., Citrus limon (L. Osveck, Plectranthus barbatus Andrews, Cymbopogon citratus (DC. Stapf, Psidium guajava L., Artemisia absinthium L., Ocimum basilicum L., Plantago tomentosa Lam., Rosmarinus officinalis L., Persea americana Mill., Aloysia citrodora Palau, Sambucus australis Cham. & Schltdl., Cuphea carthagenensis (Jacq. J.F. Macbr., Petroselinum crispum (Mill. Nyman ex A.W. Hill, Ocimum selloi Benth. e Tanacetum vulgare L., nesta ordem de Valor de Uso, foram consideradas como as mais importantes para a população estudada. As famílias mais importantes foram Asphodelaceae, Caprifoliaceae, Rutaceae e Lythraceae. Para o cálculo da porcentagem a corrigida de Concordância quanto aos Usos Principais (CUPc foram consideradas como espécies principais as que apresentaram valores acima de 24%: Eugenia uniflora, Achyrocline satureioides, Psidium guajava, Cunila microcephala, Plectranthus barbatus, Citrus × aurantium, Citrus limon, Cymbopogon citratus, Punica granatum L., Sechium edule (Jacq. Sw., Sphagneticola trilobata (L. Pruski, Aloysia citrodora, Foeniculum vulgare, Plectranthus neochilus Schltr., Artemisia absinthium, Lippia alba (Mill. N.E. Br., Mikania laevigata Sch. Bip ex Baker, Aloe arborescens e Petroselinum

  15. General Characteristics of Flora and Vegetation Formations of Eastern Anatolia Region and Its Environs (Türkiye

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    Münir ÖZTÜRK

    2015-07-01

    Full Text Available ABSTRACT: Eastern Anatolia and its environs are included in the Irano-Turanian phytogeographical region of Türkiye. The region abounds in highest mountain ranges (average 1900 m of the country some of which are of quarternary age and volcanic character. Many rivers of historical and international importance like Euphrates, Dicle, Murat, Karasu and Aras. Tigris, Aras and Çoruh flow through the region. It experiencens a typical continental climate. Soils are generally of alluvial, colluvial, chestnut-brown, regosal and basaltic types. The area embodies over 8 million ha of meadows and grasslands which is 41% of Türkiye's total pasturelands as such cattle raising is very high in this area. Forest vegetation is represented mainly by Pinus sylvestris, Quercus libani, Q. longipes, Q. brantii, Q. macranthera, Juniperus excelsa and Betula pendula. Most of these are highly degraded. Plant cover is rich in Irano - Turanian elements but we find Mediterranean and Euro-Siberian elements too to some extent, richest families being Asteraceae, Fabaceae and Poaceae, Lamiaceae, Caryophyllaceae and Apiaceae richest genera being Astragalus, Verbascum, Centaurea, Ranunculus, Alyssum, Vicia, Silene, Dianthus, Veronica and Trifolium. The number of endemics is very high, being around 950. And also the region has gene centers of genera Astragalus, Gypsophila, Cousinia, Acanthophyllum etc. The some typical associations one comes across in this area are; Triseto-Pinetum sylvestris, Trifolio-Pinetum sylvestris, Ballato-Rhamnetum pallasii, Caragano-Minuartietum lineatea, Hippophaetum rhamnoidis, Typho-Juncetum inflecii, Hordeetum violaceumii, Deschampsio-Ranunculetum brachylobus, Hordeeto-Ranunculetum comosae and Polygon-Primuletum auriculatae. Dominantly vegetation of the region is steppe. The are a lot of natural plant taxa using local people. Key Words: Eastern Anatolia, Flora, Vegetation, Biodiversity, Geobotany, Endemism, Türkiye. Doğu Anadolu Bölgesi ve

  16. Uso de Plantas com Fins Medicinais no Município de Barra – BA

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    Antonia Mirian Nogueira de Moura Guerra

    2016-04-01

    Full Text Available Este trabalho foi realizado no Assentamento de Reforma Agrária Sítio Novo, Barra–BA, objetivando conhecer as espécies medicinais e suas formas de uso adotadas pela população local. Entre Abril e Maio de 2015 foram realizadas entrevistas com aplicação de questionários estruturados. Todos os entrevistados fazem uso de plantas medicinais e cultivam alguma espécie em suas residências, usam as plantas por tradição familiar e por se tratar de um remédio natural  consomem principalmente quando estão doentes. Foram registradas 258 citações, nas quais foram relatadas a utilização de várias partes vegetais das 41 espécies, em 11 formas de preparo, com diferentes finalidades terapêuticas. As folhas e as cascas foram as partes mais utilizadas e os chás a principal forma de uso. As famílias Lamiaceae, Anacardiaceae, Myrtaceae e Rutaceae foram aquelas com maior representatividade e adoção medicinal. As espécies mais citadas foram a Cymbopogon citratus (Capim Santo – 15,5%, Lantana camara L. (Camará – 10,5%, Mentha sp.  (Hortelã Miúdo – 9,7%, Ocimum basilicum L. (Manjericão – 5,4% e Ruta graveolens L. (Arruda – 4,6% que corresponderam por 45,7% das citações. Entre as indicações terapêuticas citadas, destacam-se: doenças associadas ao sistema respiratório, indicações anti-inflamatórias, hipertensão, calmante e doenças associadas ao sistema digestivo. Nota-se que os moradores são detentores de um vasto conhecimento empírico relacionados à etnobotânica e as condições climáticas relacionadas ao manejo da flora da região e que o uso de plantas medicinais para fins terapêuticos é marcante, evidenciando que o uso popular de plantas medicinais está inserido significativamente nas esferas social e cultural dessa comunidade, o que assegurará a perpetuação dos costumes entre as próximas gerações.Use plants with medicinal purposes in Barra - BA MunicipalityAbstract: This work was carried out in the

  17. Diosas y madres, el arquetipo femenino en Diego Rivera Diosas y madres, el arquetipo femenino en Diego Rivera

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    Dina Comisarenco Mirkin

    2010-11-01

    principales aportes iconográficos al arte universal. Sus excelsas imágenes de diosas y madres nos permiten re-conectarnos con el inconciente colectivo, y particularmente con el poder tradicionalmente asociado con el arquetipo femenino, lamentablemente desatendido y denigrado en gran parte del arte contemporáneo.  In this paper I examine the representation of the feminine archetype in Diego Rivera´s murals, considering not just his learned use of traditional allegorical symbols, but also, his personal experiences, in particular those in regard to filial love, maternity/paternity, and his very complex amorous relationships with some outstanding women of his time, which in a more or less conscious way, the artist intertwined with extraordinary lyrical imagination in his ample and complex iconographic programs. I conclude that for Rivera, muralism was not only an instrument of political action, such as it is normally recognized by specialized literature, but also, an opportunity to elaborate personal themes, in which paradoxically he reaches a greater degree of universality and transcendence that the one which characterizes his history paintings.

     

  18. Efeito da aplicação de ácido giberélico no crescimento da palmeira-ráfia Effect of gibbeerllic acid application on lady palm growth

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    Armando Reis Tavares

    2007-12-01

    Full Text Available A palmeira-ráfia possui grande importância econômica devido ao seu interesse comercial. É uma planta ornamental muito apreciada por sua beleza e utilização em ambiente interno, crescendo bem em locais com sombra e apresentando resistência à exposição solar. O valor econômico dessa planta está relacionado à altura e número de hastes. O objetivo do trabalho foi promover o crescimento da planta, através da aplicação do regulador de crescimento ácido giberélico (GA3. Mudas de palmeira-ráfia com aproximadamente um ano de idade foram aspergidas a cada 21 dias (quatro aplicações com solução de ácido giberélico nas concentrações de 0; 75; 150; 225; e 300 mg.L-1. Os resultados indicaram que a aplicação do ácido giberélico foi eficiente na promoção do crescimento da espécie, sendo esse incremento significativo nas variáveis: comprimento dos pecíolos, lâminas foliares e altura da planta. Não se observou diferença no acúmulo de matéria seca ou fresca do pecíolo entre os tratamentos, e plantas do tratamento-controle foram superiores às dos tratamentos 225 e 300 mg L-1, nas variáveis diâmetro do colo e massa fresca e seca das raízes. Devido ao incremento na altura das mudas e à alteração da disposição das folhas da palmeira, a utilização do ácido giberélico pode ser recomendada para a produção de mudas de alta qualidade.Lady Palm is of great economic importance due its commercial interest. It is an ornamental plant growing in shadowy places, resistant to sun exposure and very appreciated for its beauty and ornamental value. Its commercial value is associated to plant height and shoot number. The aim of this work was to stimulate lady palm growth by applying the plant growth regulator gibberellic acid. Plantlets of R. excelsa approximately one year old were sprayed every 21 days (4 applications with gibberellic acid solution at concentrations of 0; 75; 150; 225 and 300 mg L-1. The results showed that

  19. Variabilidade genética para caracteres morfométricos de matrizes de castanha-do-brasil da Amazônia Mato-grossense Genetic variability for morphometric characteristics in brazilian nut parent trees from northern Mato Grosso, Amazon rain forest

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    Flora Ferreira Camargo

    2010-12-01

    Full Text Available O presente estudo objetivou estudar a variabilidade genética de matrizes de Bertholletia excelsa através da estimação de parâmetros e ganhos genéticos para os caracteres peso/ouriço (g, peso de sementes/ouriço (g e número de sementes/ouriço no pré-melhoramento da espécie. Foram utilizadas 90 matrizes de polinização aberta, sendo 30 matrizes de cada tipo, denominadas localmente de rajada, mirim e rosa, no município de Cotriguaçu, noroeste de Mato Grosso, região amazônica. O experimento foi estabelecido sob delineamento inteiramente ao acaso, com 90 tratamentos (matrizes e seis ouriços por matriz, com suas respectivas sementes. As variáveis foram analisadas usando-se a metodologia de modelo linear misto do software SELEGEN-REML/BLUP. Os coeficientes de herdabilidades individuais no sentido amplo dos efeitos genotípicos totais (0,21, 0,14 e 0,34 para os caracteres peso/ouriço (g, peso de sementes/ouriço (g e número de sementes/ouriço, respectivamente, são considerados moderados para os dois primeiros caracteres e alto para o caráter número de sementes/ouriço, sugerindo expressivo controle genético. A seleção das 10 melhores matrizes revelou predominância da procedência do tipo rosa, proporcionando ganhos genéticos expressivos de pelo menos 24,16% para peso/ouriço (g, 27,44% para peso de sementes/ouriço e 16,92% para o caráter número de sementes por ouriço. Os valores expressivos das matrizes do tipo rosa estimulam a utilização desses germoplasmas em programas de melhoramento genético da espécie, na seqüência das avaliações, bem como apontando para a possibilidade de obtenção de híbridos intraespecíficos para caracteres desejáveis.The goal of the study was to detect genetic variability in Brazilian nuts parent trees through parameters estimation and genetic gains for the following indexes: fruit weight (g seed weight per fruit (g and number of seeds per fruit at species pre-improvement. Ninety (90

  20. Medicinal Plants of the markets of Izúcar de Matamoros and Acatlán de Osorio, Puebla

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    David Martínez Moreno

    2016-03-01

    applied surveys showed that in the mentioned markets 31 plants are sold to cure 31 diseases, 25 at the Izúcar de Matamoros market, and 27 at the Acatlán de Osorio market. The most used part of the plants to cure the ailments are the leaves, stems and flowers, and the application is oral and local. The use of medicinal plants is to cure diseases of the respiratory, digestive, circulatory, excretory, nervous systems, as well as for the cultural syndrome. The way of selling them is as a bunch or as a compound. It is concluded that both markets are traditional and the most important sold families of plants are Asteraceae, Lamiaceae, Fabaceae and Rutaceae; the market that presented a major recorded medicinal plants was the Acatlán de Osorio with 144 species. The majority of medicinal plants in both markets are to cure diseases of the digestive, excretory, nervous, respiratory and circulatory systems as well as cultural syndrome; most of the species are wild and the most used parts of the plants are the stem, leaves, flowers and fruits.

  1. Concentración natural de compuestos antimaláricos en artrópodos tropicales (in vitro

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    Misael Chinchilla-Carmona

    2008-06-01

    Full Text Available Extractos alcohólicos, hexánicos y diclorometánicos de 751 muestras de artrópodos fueron estudiados por la presencia de actividad antimalárica. En este trabajo se empleó un modelo murino usando el Plasmodium berghei, modelo que es biológicamente similar a la malaria humana. El estudio fue realizado determinando el efecto del extracto sobre el parásito por la inclusión o no del colorante azul de cresil brillante. Estimando como positivos aquellos extractos cuya actividad antimalárica se mostró en concentraciones no mayores de 50 mg, se encontró que los órdenes más promisorios fueron Lepidoptera (24.1%, Polydesmida (81.3%, Blattodea (25% y Opiliones, entre otros. Las formas inmaduras de Lepidoptera fueron las más positivas, por lo que se analizaron las plantas hospederos de donde se alimentaban dichos organismos. Las familias de estas plantas eran Malvaceae, Acanthaceae, Rutaceae, Myrtaceae, Solanaceae, Fabaceae, Urticaceae, Anacardiaceae, Rosaceae, Asteraceae, Rubiaceae, Lauraceae y Caprifoliaceae. Especies de casi todas estas familias han sido reportadas con actividad antimalárica. En el caso de los órdenes Polydesmida, Opiliones y Blattodea, cuyas formas adultas presentaron alguna actividad contra P. berghei, encontramos que todos esos grupos se alimentan también de plantas. En el caso de Opiliones sus especies son predadores de lepidópteros, coleópteros, hemípteros fitófagos y otros artrópodos, además de que producen sustancias de defensas tales como alcoholes, cetonas y quinonas, entre otros, todo lo cual podría explicar la actividad encontrada. Algunas especies del Orden Polydesmida, también secretan ciertas sustancias químicas, las cuales podrían tener un efecto antiparasitario. Así, a través de este trabajo en artrópodos hemos llegado a identificar fuentes vegetales potenciales para componentes antimaláricos.Natural concentration of antimalaric components in Tropical arthropods (in vitro. Alcohol, hexane and

  2. Ethnopharmacological survey on medicinal plants used in snakebite treatments in Western and Sabaragamuwa provinces in Sri Lanka.

    Science.gov (United States)

    Dharmadasa, R M; Akalanka, G C; Muthukumarana, P R M; Wijesekara, R G S

    2016-02-17

    Sri Lanka has a great diversity of snake species. In this relation, over 40,000 cases of snakebite accidents are reported annually from different agro-ecological regions of the country. Since more than 95% of victims rely on traditional treatments, there is an urgent necessity to improve the system. Traditional knowledge on snakebite treatments has been passed on from generation to generation within families. Unfortunately, there has been a limited update of information on pertinent issues related to this subject. In the present study we conducted a comprehensive survey on the types of medicinal plant materials, including the specific plant parts that are available for this purpose. In addition, various treatment types, frequency index, heavily used and rare materials, family wise distribution, challenges faced by traditional practitioners and future prospects were also explored. The present survey covered two provinces with a high population of traditional practitioners for snakebites treatment in Sri Lanka.Information was gathered from a total of seventy-four (74) traditional practitioners from the Sabaragamuwa and Western provinces. A questionnaire was prepared and pre-tested by 10-15 respondents prior to the survey. Actual data were gathered through face-to-face interviews. Collected data were tabulated and analyzed. A total of 341 different plant species belonging to 99 families were documented. The highest number of plants was reported from the family Fabaceae (32 species). This was followed by Malvaceae (16 species), Asteraceae (15 species), Rutaceae (13 species Apocyanaceae (14 species), Lamiaceae (11 species), Poaceae, Euphorbaceae and Phyllanthaceae (10 species per each) respectively. Different parts of the plant such as leaves (53.67%), barks (26.10%), entire plant (14.08%), roots (10.26%), bulbs (8.80%), seeds (7.62%), fruits (6.45%), buds (5.87%), flowers (3.23%) stems (2.93%) and latex (2.05%) were used for the preparation of nine different types of

  3. Norepinephrine alkaloids as antiplasmodial agents: Synthesis of syncarpamide and insight into the structure-activity relationships of its analogues as antiplasmodial agents.

    Science.gov (United States)

    Aratikatla, Eswar K; Valkute, Tushar R; Puri, Sunil K; Srivastava, Kumkum; Bhattacharya, Asish K

    2017-09-29

    Syncarpamide 1, a norepinephrine alkaloid isolated from the leaves of Zanthoxylum syncarpum (Rutaceae) exhibited promising antiplasmodial activities against Plasmodium falciparum with reported IC 50 values of 2.04 μM (D6 clone), 3.06 μM (W2 clone) and observed by us 3.90 μM (3D7 clone) and 2.56 μM (K1 clone). In continuation of our work on naturally occurring antimalarial compounds, synthesis of syncarpamide 1 and its enantiomer, (R)-2 using Sharpless asymmetric dihydroxylation as a key step has been accomplished. In order to study structure-activity-relationship (SAR) in detail, a library of 55 compounds (3-57), which are analogues/homologues of syncarpamide 1 were synthesized by varying the substituents on the aromatic ring, by changing the stereocentre at the C-7 and/or by varying the acid groups in the ester and/or amide side chain based on the natural product lead molecule and further assayed in vitro against 3D7 and K1 strains of P. falciparum to evaluate their antiplasmodial activities. In order to study the effect of position of functional groups on antiplasmodial activity profile, a regioisomer (S)-58 of syncarpamide 1 was synthesized however, it turned out to be inactive against both the strains. Two compounds, (S)-41 and its enantiomer, (R)-42 having 3,4,5-trimethoxy cinnamoyl groups as side chains showed better antiplasmodial activity with IC 50 values of 3.16, 2.28 μM (3D7) and 1.78, 2.07 μM (K1), respectively than the natural product, syncarpamide 1. Three compounds (S)-13, (S)-17, (S)-21 exhibited antiplasmodial activities with IC 50 values of 6.39, 6.82, 6.41 μM against 3D7 strain, 4.27, 7.26, 2.71 μM against K1 strain and with CC 50 values of 147.72, 153.0, >200 μM respectively. The in vitro antiplasmodial activity data of synthesized library suggests that the electron density and possibility of resonance in both the ester and amide side chains increases the antiplasmodial activity as compared to the parent natural product 1

  4. A systematic revision of Baconia Lewis (Coleoptera, Histeridae, Exosternini

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    Michael Caterino

    2013-10-01

    Full Text Available Here we present a complete revision of the species of Baconia. Up until now there have been 27 species assigned to the genus (Mazur, 2011, in two subgenera (Binhister Cooman and Baconia s. str., with species in the Neotropical, Nearctic, Palaearctic, and Oriental regions. We recognize all these species as valid and correctly assigned to the genus, and redescribe all of them. We synonymize Binhister, previously used for a polyphyletic assemblage of species with varied relationships in the genus. We move four species into Baconia from other genera, and describe 85 species as new, bringing the total for the genus to 116 species. We divide these into 12 informal species groups, leaving 13 species unplaced to group. We present keys and diagnoses for all species, as well as habitus photos and illustrations of male genitalia for nearly all. The genus now contains the following species and species groups: Baconia loricata group [B. loricata Lewis, 1885, B. patula Lewis, 1885, B. gounellei (Marseul, 1887a, B. jubaris (Lewis, 1901, B. festiva (Lewis, 1891, B. foliosoma sp. n., B. sapphirina sp. n., B. furtiva sp. n., B. pernix sp. n., B. applanatis sp. n., B. disciformis sp. n., B. nebulosa sp. n., B. brunnea sp. n.], B. godmani group [B. godmani (Lewis, 1888, B. venusta (J. E. LeConte, 1845, B. riehli (Marseul, 1862, comb. n., B. scintillans sp. n., B. isthmia sp. n., B. rossi sp. n., B. navarretei sp. n., B. maculata sp. n., B. deliberata sp. n., B. excelsa sp. n., B. violacea (Marseul, 1853, B. varicolor (Marseul, 1887b, B. dives (Marseul, 1862, B. eximia (Lewis, 1888, B. splendida sp. n., B. jacinta sp. n., B. prasina sp. n., B. opulenta sp. n., B. illustris (Lewis, 1900, B. choaspites (Lewis, 1901, B. lewisi Mazur, 1984], B. salobrus group [B. salobrus (Marseul, 1887b, B. turgifrons sp. n., B. crassa sp. n., B. anthracina sp. n., B. emarginata sp. n., B. obsoleta sp. n.], B. ruficauda group [B. ruficauda sp. n., B. repens sp. n.], B. angusta group [B

  5. A systematic revision of Baconia Lewis (Coleoptera, Histeridae, Exosternini).

    Science.gov (United States)

    Caterino, Michael S; Tishechkin, Alexey K

    2013-01-01

    Here we present a complete revision of the species of Baconia. Up until now there have been 27 species assigned to the genus (Mazur, 2011), in two subgenera (Binhister Cooman and Baconia s. str.), with species in the Neotropical, Nearctic, Palaearctic, and Oriental regions. We recognize all these species as valid and correctly assigned to the genus, and redescribe all of them. We synonymize Binhister, previously used for a polyphyletic assemblage of species with varied relationships in the genus. We move four species into Baconia from other genera, and describe 85 species as new, bringing the total for the genus to 116 species. We divide these into 12 informal species groups, leaving 13 species unplaced to group. We present keys and diagnoses for all species, as well as habitus photos and illustrations of male genitalia for nearly all. The genus now contains the following species and species groups: Baconia loricata group [Baconia loricata Lewis, 1885, B. patula Lewis, 1885, Baconia gounellei (Marseul, 1887a), Baconia jubaris (Lewis, 1901), Baconia festiva (Lewis, 1891), Baconia foliosoma sp. n., Baconia sapphirina sp. n., Baconia furtiva sp. n., Baconia pernix sp. n., Baconia applanatis sp. n., Baconia disciformis sp. n., Baconia nebulosa sp. n., Baconia brunnea sp. n.], Baconia godmani group [Baconia godmani (Lewis, 1888), Baconia venusta (J. E. LeConte, 1845), Baconia riehli (Marseul, 1862), comb. n., Baconia scintillans sp. n., Baconia isthmia sp. n., Baconia rossi sp. n., Baconia navarretei sp. n., Baconia maculata sp. n., Baconia deliberata sp. n., Baconia excelsa sp. n., Baconia violacea (Marseul, 1853), Baconia varicolor (Marseul, 1887b), Baconia dives (Marseul, 1862), Baconia eximia (Lewis, 1888), Baconia splendida sp. n., Baconia jacinta sp. n., Baconia prasina sp. n., Baconia opulenta sp. n., Baconia illustris (Lewis, 1900), Baconia choaspites (Lewis, 1901), Baconia lewisi Mazur, 1984], Baconia salobrus group [Baconia salobrus (Marseul, 1887b), Baconia

  6. Historia de los Medicamentos

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    Alfredo Jácome Roca

    2003-10-01

    entonces el hombre más poderoso de la tierra– dice las siguientes palabras que fueron consignadas en sus “Memorias”, libro de Marguerite Yourcenar: “Es difícil seguir siendo emperador ante un médico, y también es difícil guardar la calidad de hombre. El ojo de Hermógenes sólo ve en mi un saco de humores, una triste amalgama de linfa y de sangre… pero ya no cuento, como Hermógenes finge contar, con las virtudes maravillosas de las plantas y el dosaje exacto de las sales minerales que ha ido a buscar a Oriente”. “Perdono a este buen servidor su esfuerzo por disimularme la muerte… tendré suerte de ser el mejor atendido de los enfermos… pero nada puede exceder de los límites prescritos… mis piernas hinchadas ya no me sostienen…”. La hidropesía llevó a la muerte a este emperador romano, en quien se conjugaron las más excelsas virtudes y los peores vicios, y en pos de una cura imposible, ofrecida por la medicina mágica, no dudó en sacrificar a Antinoor, el mancebo amado, para evitar la llegada de la parca. A pesar de su poder y de su dinero, su fallecimiento ocurrió a la edad de 62 años. Los árabes aportan mucho a la farmacia.

    La alquimia, aún con sus errores, es un paso adelante. Aparecen pioneros listados de hierbas medicinales y las primeras boticas. Se respeta y mejora en algo el conocimiento de la antigüedad clásica, se introducen los jarabes, el alcohol, y muchas drogas nuevas, incluso la misma palabra “droga” que designa a los medicamentos. De los territorios del Islam pasa el “Ars Medica” a la Escuela de Salerno. La medicina y los medicamentos se conservan en la Edad Media a través de los monjes copistas y cultivadores de sus jardines botánicos. En el “Regimen Sanitatis Salernitanum” se lee el siguiente verso sobre la “Salvia salvatrix”, cuyo original está por supuesto, en latín:

    “¿Por qué ha de morir el hombre
    que en su huerto tiene salvia?
    Para el venir de la muerte
    No hay

  7. ACADÉMICO CORRESPONDIENTE. GUSTAVO CAMARGO VEGA (1932- 2016

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    Jaime Eraso López

    2016-12-01

    Full Text Available

    Palabras del Dr. Jaime Eraso López ante el féretro del Dr. Gustavo Camargo Vega en la parroquia de la Sagrada Familia de Palermo el día 30 de agosto de 2016.

    Por espacio de muchos años desde su llegada a Pasto y su inmediata vinculación al Hospital San Pedro, en su calidad de especialista en Urología, formando parte de un equipo de inolvidables colegas, tuve la inmensa fortuna de ser amigo de un hombre noble, querido y admirado por todos quienes lo conocimos, Gustavo Camargo Vega. Un gran ser humano cuya grandeza en la vida estribó en lo que hizo y que al pasar a la eternidad, llamado por Dios, nos ha dejado atribulados, con ese dolor en el alma que invade a Beatriz, su esposa, y a toda su familia, a todos sus amigos, a sus numerosos pacientes y a sus compañeros académicos con los que compartió como Miembro de la Junta Directiva del Capítulo de Nariño de la Academia Nacional de Medicina de Colombia, mientras me desempeñaba como Presidente, aprovechando todo su talento para la buena marcha de la Institución.

    Ante mis inquietudes intelectuales, estando enfermo él, siempre tuvo la gentileza de llamarme por teléfono para expresarme con nobleza sus generosas palabras de aliento y, a raíz de la publicación de mi último libro sobre la vida del Padre Jaime Álvarez, me expresaba su identificación con las ideas de los prologuistas de la obra, demostrando así la grandeza de su alma. Cuando lo visitamos en su lecho con el Académico Dr. Leonardo Matta hace un par de meses, con gran lucidez recordábamos esa lejana época, desgraciadamente ya desaparecida, en la que servíamos al Hospital con un sentido verdaderamente humano hacia nuestros enfermos.

    Gustavo fue una persona de excelsas virtudes ciudadanas. Deja una cicatriz de luz sobre la faz de esta región donde ejerció su labor de amor y de saber, con su carisma, con su sonrisa, con su señorío. Señalo su saber, porque sus

  8. El síndrome afectivo hipertímico

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    Roberto Anselmo Ramos Valverde

    2014-08-01

    , excelsa autoestima; cumplen las normas educativas, laborales y sociales; las relaciones interpersonales pueden ser normales o afectarse de manera significativa, cuando aparecen eclosiones disfóricas e irritables. Es frecuente en la práctica clínica que la primera visita al médico ocurra largo tiempo después del debut y que se aqueje insomnio, no funcionan a nivel psicótico ni requieren hospitalización. Adquiere un puntaje de 7 a 20 en la escala de Young para la manía. (2 Descripción general: puede destacarse el maquillaje, el vestuario ser provocativo y exagerar el uso de joyas y adornos. Funciones de síntesis: vigilia normal, atención con tendencia a la hipervigilancia, memoria normal o ligeramente incrementada, orientación normal. Funciones de relación: pueden estar normales o encontrarse autoestima incrementada en la relación consigo mismo, sociabilidad, autoritarismo y hostilidad con los demás, interés por la recreación, por la diversión y disposición incrementada a la actividad en la relación con las cosas. Capacidades intelectuales conservadas. Funciones cognoscitivas: sensopercepciones sin alteraciones; pensamiento con ligera aceleración en su curso. Funciones afectivas: hipertimia discreta, labilidad afectiva, irritabilidad y disforia. Conducta: hiperbulia, hiperkinesia que no compromete la productividad y favorece la creatividad; necesidades con insomnio, puede aparecer hipererotismo, bulimia e hipersociabilidad, que supera ligeramente la normalidad cultural. Este síndrome conduce al diagnóstico del trastorno hipomaniaco, a la ciclotima, a los trastornos bipolares tipo I y II y a los trastornos del estado de ánimo orgánicos. (2 Síndrome afectivo hipertímico subsíndrome maniaco El funcionamiento psíquico de este síndrome es claramente psicótico, con necesidad de hospitalización en la mayoría de los casos. Los mecanismos reguladores de la personalidad fracasan, comportándose los pacientes de manera irresponsable, imprudente

  9. Éticas teleológicas y terrorismo islamista

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    Sanmartín Esplugues, José

    2012-06-01

    Taymiyya y Abul alla Maududi fija como meta suprema de la vida del hombre la complacencia de Allah y como medio principal para alcanzar esta meta la aplicación plena de la sharía o legislación divina. Se sustenta que esta aplicación, que configura la forma de vida del musulmán auténtico, sólo se realizó de forma adecuada entre el año 622 y el 660, en tiempos del Profeta y los primeros cuatro califas (los llamados «píos antepasados» o «salaf». De modo que la ética del terrorismo islamista prefigura como futuro una utopía regresiva, para la que fijan ahora como enemigos tanto a los infieles (el enemigo lejano como a los apóstatas (el enemigo cercano. La interiorización de los principios de esta ética y, en general, de las ideas vertebradoras de la filosofía que constituye la base del terrorismo islamista (el denominado «qutbismo», del nombre de su principal representante, Sayyid Qutb correlaciona con distorsiones cognitivas, emocionales y comportamentales que presentan los terroristas, en particular: percibirse y sentirse como soldados perte-necientes a una vanguardia de musulmanes auténticos, que cumplen con el deber religioso (el mandato divino de combatir a quienes contribuyen con sus ideas y prácticas a la degradación de los valores del Islam, tal como se fijan en los textos sagrados. Se trata, consideran, de una guerra justa, porque, aun cuando tenga la apariencia de ofensiva, los soldados de la vanguardia la llevan a cabo en defensa del auténtico Islam, amenazado en su esencia por apóstatas e infieles. A sus aparentes víctimas, concluyen, habría que verlas, pues, como lo que realmente son: los verdugos de una forma de vida tan excelsa como la auténticamente musulmana.

  10. The Role of Rootstock in Antioxidant Activity of Citrus Fruit: Comparison of Antioxidant Activity of The Fruits of Two Commercial Citrus Varieties With The Fruits of Four Different Rootstocks

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    N . Hemmati

    2016-02-01

    Full Text Available Introduction: all fruits that called citrus are from rutaceae family and aurantioideae subfamily. This subfamily have more than 33 different genus that only three of its genus (citrus, poncirus and fortunella have economic aspects and in citrus producing country are important. It's reported that orange skin has a phenolic compounds which play a role in natural defense mechanism. Also various compounds of phenolic and antioxidant have a major role in fruit tolerance to stressful condition suh as cold and drought. Metabolites found in citrus fruits have antioxidant properties and it's very useful in pharmaceutical, food and cosmetics industries. Oranges, like other citrus fruits, are an excellent source of vitamin C; Vitamin C is a powerful natural antioxidant. Consumption of foods rich in vitamin C helps the body develop resistance against infectious agents and scavenge harmful, pro-inflammatory free radicals from the blood. Various factors such as rootstock type can effect on quality and quantity of citrus fruits. Also, the usage of rootstock causes the change in plant characteristics such as flowering time, ripening time, fruit quality and antioxidant characters of the fruits. Other factors except the rootstock such as scion, geographical and climate factors are effective on producing secondary metabolites. Also active substances or secondary metabolites are producing by the conduction of genetic processes, but their production are being effected by other factors obviously. The aim of this study is to investigating the biochemical changes grafted tree fruit that affected by rootstock with study the correlation between grafted tree and rootstock changes. Materials and Methods: This study was done to compare the amount of total phenol, total flavonoids and antioxidant features of fruit flesh and skin with investigating the effect of cultivar and rootstock on these parameters based on completely randomized factorial design with three replications

  11. Antimalarial activity of selected Sudanese medicinal plants with emphasis to Maytenus senegalensis

    Energy Technology Data Exchange (ETDEWEB)

    Idris, Ahmed El Tahir Mohamed [Department of Biochemistry, Faculty of Medicine, University of Khartoum, Khartoum (Sudan)

    1998-03-01

    The aim of the present study is to identify and characterize the antimalrial agents from traitional Sudanese medicinal plants. 49 plants parts representing 26 species from 15 families were extracted and screened for their in vitro antimalrial activity using P. falciparum strain 3D7 which is chloroquine sensitive and Dd2 strain which is chloroquine resistant and pyrimethamine sensitive.The plant species investigated exhibited diverse botanical families. They includes Annonaceae, Aristolochiaceae, Asteraceae, Balantiaceae, Caesalpiniceae, Celasteraceae, Cucurbitaceae, Fabaceae, Graminae, Meliaceae, Myrtaceae, Polygonaceae, Rubiaceae, Rutaceae, and simaroubaceae. The evaluation of these plants for their antimalarial activity and their effect on lymphocyte proliferation was carried out. 57 extracts were tested on the chloroquine sensitive strain (3D7). Where 34 extracts (59%) exhibited significant activity against 3D7 with IC{sub 50} values {<=} 50 {mu} g/ml. While 21 extracts (57%) showed antimalrial activities with IC{sub 50} values {<=} 50 {mu} g/ml on Dd2. 13 extracts (22%) and ten extracts (18%) only showed an activity with IC{sub 50} values {<=} 5 {mu} g/ml on 3 D7 and Dd2, respectively. The activities of some plant extracts, which affected 3D7 strain, were measured using the radiolabelled ({sup 3}H) hypoxanthine method and microscopical count. 15 plant extracts (48%) from 32 showed IC{sub 50} values {<=} 50 {mu} g/ml against 3D7 strain using the radiolabelled hypoxanthine methods and only 5 extracts (16%) showed IC{sub 50} values {<=} 5 {mu} g/ml against 3D7. Most of the extracts screened had a low effect on lymphocyte proliferation (IC{sub 50} values >100 {mu} g/ml), where as Sonochous cornatus, Balanites aegyptiaca, Tamarindus indica, Acacia nilotica, Annona squamosa, Eucalyptus globulus and Cassia tora enhanced lymphocyte proliferation. liquid-liquid partition of methanolic preparation of Acacia nilotica seeds and husk showed that the ethylacetate phase

  12. Antimalarial activity of selected Sudanese medicinal plants with emphasis to Maytenus senegalensis

    International Nuclear Information System (INIS)

    Idris, Ahmed El Tahir Mohamed

    1998-03-01

    The aim of the present study is to identify and characterize the antimalrial agents from traitional Sudanese medicinal plants. 49 plants parts representing 26 species from 15 families were extracted and screened for their in vitro antimalrial activity using P. falciparum strain 3D7 which is chloroquine sensitive and Dd2 strain which is chloroquine resistant and pyrimethamine sensitive.The plant species investigated exhibited diverse botanical families. They includes Annonaceae, Aristolochiaceae, Asteraceae, Balantiaceae, Caesalpiniceae, Celasteraceae, Cucurbitaceae, Fabaceae, Graminae, Meliaceae, Myrtaceae, Polygonaceae, Rubiaceae, Rutaceae, and simaroubaceae. The evaluation of these plants for their antimalarial activity and their effect on lymphocyte proliferation was carried out. 57 extracts were tested on the chloroquine sensitive strain (3D7). Where 34 extracts (59%) exhibited significant activity against 3D7 with IC 50 values ≤ 50 μ g/ml. While 21 extracts (57%) showed antimalrial activities with IC 50 values ≤ 50 μ g/ml on Dd2. 13 extracts (22%) and ten extracts (18%) only showed an activity with IC 50 values ≤ 5 μ g/ml on 3 D7 and Dd2, respectively. The activities of some plant extracts, which affected 3D7 strain, were measured using the radiolabelled ( 3 H) hypoxanthine method and microscopical count. 15 plant extracts (48%) from 32 showed IC 50 values ≤ 50 μ g/ml against 3D7 strain using the radiolabelled hypoxanthine methods and only 5 extracts (16%) showed IC 50 values ≤ 5 μ g/ml against 3D7. Most of the extracts screened had a low effect on lymphocyte proliferation (IC 50 values >100 μ g/ml), where as Sonochous cornatus, Balanites aegyptiaca, Tamarindus indica, Acacia nilotica, Annona squamosa, Eucalyptus globulus and Cassia tora enhanced lymphocyte proliferation. liquid-liquid partition of methanolic preparation of Acacia nilotica seeds and husk showed that the ethylacetate phase possessed the highest activity against both 3D7 and Dd2

  13. Presentación del Libro: “Juan de Dios Carrasquilla, Hombre de Ciencia”

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    Adolfo De Francisco Zea

    2004-06-01

    conducen, me quedará al menos la satisfacción de haber trabajado tenazmente por hallar la verdad”.

    En el libro que presento esta noche, he intentado analizar un tanto la noble personalidad del doctor Carrasquilla; señalar sus constantes esfuerzos por alcanzar la verdad en las diversas empresas en que se comprometió a lo largo de su meritoria existencia; recordar sus aciertos y sus posibles faltas, mencionar su perseverancia en las investigaciones que emprendía, y destacar, además, esa dignidad personal suya que le fue tan característica, que le permitía recibir sin arrogancia las alabanzas y aceptar sin pesadumbre las críticas que fueran razonables.

    En la Introducción a la semblanza de su sobresaliente vida digo las siguientes palabras: “Cuando hace algunos años escribía la biografía de mi abuelo el doctor Luis Zea Uribe, me encontré con el Discurso que pronunciara en el sepelio del doctor Juan de Dios Carrasquilla el 15 de julio de 1908. Las palabras del doctor Zea Uribe en esa dolorosa ocasión señalaban dos de las características más sobresalientes del ilustre médico desaparecido: su honestidad y su bondad. A partir de esos días, mi interés se encaminó a descubrir las razones que tuvo el doctor Zea para describir con esas dos hermosas palabras la personalidad del doctor Carrasquilla; quizás porque para mí son vocablos que representan dos virtudes excelsas del hombre: la honestidad que es la esencia del hombre moral, y la bondad que es la sublimación de todas las demás fortalezas del alma.

    Intenté en consecuencia aproximarme a todo aquello que pudiera llevarme a descubrir los propósitos del doctor Zea Uribe al pronunciar sus elocuentes palabras; a penetrar con curiosidad, así fuera fugazmente, en la vida y la obra del hombre al que muchos de sus contemporáneos conocieron con el apelativo de “sabio”, para indagar con serenidad y sin pasiones en los aconteceres de su existencia y en sus ejecutorias más notables